LOCUS       DASTNK010000001        14038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_741944, whole genome shotgun sequence.
ACCESSION   DASTNK010000001 DASTNK010000000
VERSION     DASTNK010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14038
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741944"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VFR20_00005"
     CDS             <1..666
                     /locus_tag="VFR20_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEU0228928.1"
                     /translation="QLAQWAENRVTLSRLFDAAGAITDACPAGIYLDRRGLSIPNSDA
                     LRFMPKLDYWHDGQLLGTFPAMLAAVTSPSGALVSVHRTYITPEGHKAPVPTVKKLMA
                     TAGPMAGTSIKIGAPLARPDGRLGLGVAEGIETALAASILSGVAVWSCVSAHGLEAFE
                     PPGDVHYLYCFGDHDASGVGQKAASDLAHRAARSGLSARIWIPEAPGTDWNDELISRR
                     ASV"
     gene            663..2453
                     /locus_tag="VFR20_00010"
     CDS             663..2453
                     /locus_tag="VFR20_00010"
                     /inference="COORDINATES: protein motif:HMM:NF017826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HEU0228929.1"
                     /translation="MTEAQIVELKALDVDEPDQADPFPSMGDRPCWRCYDEAIERDGK
                     KVRPGVHYHGIKESKGDAPPTITDTWHCGPLHIESLTANQEDGDHGLLLRFRNVNRNW
                     KKWAMPKQMLAGDGTEVLSVLYADGLDIDRKSKARVLDYINSQHPGKRLRAASTTGWH
                     GGAFVLPDEVIGASDIWFQATERTAPYGTAGTFEGWKEQVATRATGNPMLTLGLSAAL
                     AGPLLEKLNIGGGGLHLFGDSSKGKTSILMAAVSVWGGPAFLRTWRTTSNGLEGAARM
                     HTETLLPLDEINQCEPKQLYEAAYELINGQGKTRANVKGEARQAARWRVFVLSNGEMS
                     IPARIAEGGMRVKAGQTLRILDVPATSQTHGAFDDLHGFASGAALSDAIRDGAARHYG
                     HAGRAFVEALVAQFSEGLELSDAMGIITARMAPEGGQETRAARVFALCGLAGEMATGA
                     GIVPWREGQAGEAALLGFNLWRNQRGTGGRNAEDAAILRMVADFIDKHGDSRFSGLDD
                     HNAPPVRDRAGYWKDGGGGRQYLLTSGGLSDATKGYDMGRVIRALDGAQAFAQKGANG
                     KTALAVRTPDGGVKKLYHLDAAKLQEVDHE"
     gene            2721..3368
                     /locus_tag="VFR20_00015"
     CDS             2721..3368
                     /locus_tag="VFR20_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0228930.1"
                     /translation="MMRPDLLAGLYRWADAAKPEPAPNPCTSEQVSRLTKVSIPTTTA
                     VLRKVEVPASPMGLRKAAHTPAPANMGKPDADALAPVATQVATVSVVSTAEVTKLVTT
                     EKAVRSTVRTATAPRPNNPKLRDITEPVSDADQRLVDMSVRRFAGKGIPMLKARKLAE
                     RLLRALREDDDRRSCAECESFRAGRCVQGRQPFGGGGIEVLHRCTGFRLDGGNHD"
     gene            3361..3696
                     /locus_tag="VFR20_00020"
     CDS             3361..3696
                     /locus_tag="VFR20_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU0228931.1"
                     /translation="MIDALIAGKLYGVPKQGTGKNGNTYATAKVKVATGSGDMLLCGV
                     IAFDEKAQAALLALTDGDSVALSGALTPKVYQARDGEHRPGLDMVAQVVTTAYHVRHK
                     RAAIEGGDA"
     gene            3736..4782
                     /locus_tag="VFR20_00025"
     CDS             3736..4782
                     /locus_tag="VFR20_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0228932.1"
                     /translation="MRYYELNLYQPGVIPGTTKPFRTWTTHPGGQFDPGALMLEMDLI
                     VLPYAVASGGSTITLHGVAMEDLNQSQQFGMHPDAQGIEQPGMTLELRGGMKAGLPLA
                     NPAQADALIVGQIFQSYGNWRGTDMRLDLVVYPPGYTMDNPGNFVLNWKRGQKLSDAL
                     GACLAVVYPAMPIRINISGRIVADQDMPHFCSTLEELAQTVKDTTKSHYLGKTYPGVD
                     IAIHGGVIDVFDGTVPAGVVQLNFTDLVGQPTWVDKATVQFDTVLRGDIQIGSVIKAP
                     QGMTNQPGMVVSSQASLRSGANNALTFVGDFKVTGVRHVGNSRSPNAGDWATFIQATP
                     IIPTPGTVTNGVAR"
     gene            4827..5189
                     /locus_tag="VFR20_00030"
     CDS             4827..5189
                     /locus_tag="VFR20_00030"
                     /inference="COORDINATES: protein motif:HMM:NF041373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HGGxSTG domain-containing protein"
                     /protein_id="HEU0228933.1"
                     /translation="MPTEVRLSRLRAETQRCREQLSAADVDPDSELGHKLMAKLQATG
                     GTLCLAQTREGHPCRMLGDGKGGRCKFHGGKSTGPRTVEGLIRSASNQANWHRNRLGM
                     PLVGVPGVDTGGGESRQK"
     gene            6459..7739
                     /locus_tag="VFR20_00035"
     CDS             6459..7739
                     /locus_tag="VFR20_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEU0228934.1"
                     /translation="MGLSDTACRTAKVKDSDITKDGKKKSSIKLPDGTGLYLMVNRAG
                     KKYWRYNYRFGGKSKTIAYGRYPSMTLAAARDAHAAAQRELSAGRDPMTVKKSVKQAA
                     LLAAENSFKSVAQKWLAQKAPTWSATHEEKTRQLLERDVYPVFGAEPIHSITAAQVLA
                     MARGIEARGNVEMPRRSISAVGQVLRYAIGLGLIQNDPSQGMAGQLNDRPPVQHYAHV
                     GTKELPGLLRALDALPVDPTVLAVRLDMLTFVRGGELRNAEWSEFDIEAKVWRVPPGR
                     MKGRKWAKENGEAHIVPLAPQAVAILKELGAFTGHYALLFPGRDDPTQGITGAAMAKV
                     WARLGLRGQQTTHGLRGLASTQANGARRPDGSRLFDDRLIEAQLSHKMRDRIKGSYDH
                     AEHIEARTRLMLWWADYLDTLRAGGNVIPLTRQA"
     gene            7783..8082
                     /locus_tag="VFR20_00040"
     CDS             7783..8082
                     /locus_tag="VFR20_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU0228935.1"
                     /translation="MPHLIQQTESFAAWHTGLRDLRAKVAIARRIERVAAGTLGDVKS
                     LGNDLSELRVDIGPGYRVYFTMRGGVLVILLAGGDKRTQAADIKQARKLAMEIDP"
     gene            8079..8423
                     /locus_tag="VFR20_00045"
     CDS             8079..8423
                     /locus_tag="VFR20_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="HEU0228936.1"
                     /translation="MKAKSKLIPFDMTAHLDSPEAIAEYLTQVLADCDADELLRALGH
                     IAKARGMAQVAEASGLGRESLYKALAPGAKPRYDTILKVTRALGVRLTAQIVENPAPV
                     KPKQATHAAVAA"
     gene            8525..9220
                     /locus_tag="VFR20_00050"
     CDS             8525..9220
                     /locus_tag="VFR20_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0228937.1"
                     /translation="MVKPRKLTLRQAVKETKMPVMRLGDLLVYQFGGVIVGAFMPNAT
                     DWSIHDFGQPETENYIRQLYADDGWIESITHFDDEALALHHNYTLGLWVGRKFSGSGG
                     DAQRFEVDCAQATELLGPNPSREMVLSAIRIVLMMHKKYLLSARTGARFEAGRKPNTE
                     GPISKAMGSVIKRNPTIKNADLWNAMKSSPPRGWTFYDNDQGKYIEGPRGGQNMGYDR
                     FRNIAAQKRKAQK"
     gene            9562..10308
                     /locus_tag="VFR20_00055"
     CDS             9562..10308
                     /locus_tag="VFR20_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEU0228938.1"
                     /translation="MATTKQKDRIPVKDATLTTSLNHTPNLVHLRKGVPITDSLAIAR
                     EFGRSHKNVLRTLDDLIGDGTISRLNFEPAEYVDEQGKPRRMIELDDRAALVAMPFIG
                     GRKSRLGQVHLVNAFLAQRDLLSKTWTPQIIAARREAATGYRTMSAVLQMRRTEDGKE
                     TPRHVYSNEARLLNFALSGSFEAMDRETLSEQDLRALTSLEVRNSAMIAMGRPYAERK
                     FDLMRYAEGLRIPRLTSSATASIGMGGTHV"
     gene            10301..10540
                     /locus_tag="VFR20_00060"
     CDS             10301..10540
                     /locus_tag="VFR20_00060"
                     /inference="COORDINATES: protein motif:HMM:NF025456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0228939.1"
                     /translation="MSKKSTSGEAQRQRILEALDPNPKTSYDLRRMGCYQCPTRIYEL
                     RRMGYDIRTQRVSIWDEDGYQHHGIALYALVKGKK"
     gene            10537..12480
                     /locus_tag="VFR20_00065"
     CDS             10537..12480
                     /locus_tag="VFR20_00065"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU0228940.1"
                     /translation="MTTKTIHKIEVDSSQFKAFYDLFKQYSAALDDMPEEWKRVNGEV
                     SDAGKSMEDFATASGVSKESLAMAAAQAKIISDAVAGVAKGTHAGTKAQKDFLVSLHG
                     SGKAMREMAKDAKKLGSEVFGIGRYLLKIGVLGSGVAGLGGILSGLGLADLARSAVAG
                     QRDARGIGVSVGQLQAFRTDFGRYADPGVLNRMADAQGDMAKWGYLSLATGTPLGQIP
                     GANPVALSLAMMRRAHDWWDKTPVSQRTANNLQITGLNQFMSFEEVRRLGAMTNGEFA
                     QARNRFASDARNLNLSGREVDAWNTFIVEVKNAGMALDTDLKRKLAELGPSMGGLIET
                     LSTDGRQLIDEVLTPANINNLKRDIDGFTNLLGSPEFHNDIREFIQFVGDAAHGLKVL
                     AEWLDPSLKSSGNQLSVPGTGAAINGLDPGSSTGHSIWHDLASPGDMVNDGLSQLGRK
                     AKALEGKSRDKAYDDASLWGKLNMDFGGRKSTPTPAQVPKKELYLYNLEKKYGLPHGI
                     LDSTWFVESSRGVNAGPSGSGALGDFQFLKATGRQYGLINDTDRTDFHKSAAAAASYY
                     ADLKRKFHGDMKKAIAAYFDGPANVAGNYAKQGGKMPPQTQHYVDKTLSTWRANRETI
                     IKVVVTNSTGSNVAVSTNAGALS"
     gene            12477..13034
                     /locus_tag="VFR20_00070"
     CDS             12477..13034
                     /locus_tag="VFR20_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0228941.1"
                     /translation="MSLHHIKTVLDRMERPRLAQQGAFDDNNPVGKTTLTGFGDLCSS
                     WRMNRRPLAVFHSQAMSPLGGDGGNTFGYAGTHERRFANLAIRCPPSFGDDAGGSTAH
                     GGPDMAALPTSAHTAHFPLTEKSARRAARQWFTGTPTATLATWCDDRCRAHCDSLPEN
                     PARTQAFHDAFAAEIGSIIAGGVPS"
     gene            13031..13234
                     /locus_tag="VFR20_00075"
     CDS             13031..13234
                     /locus_tag="VFR20_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0228942.1"
                     /translation="MNDHTTMRLEDHRADIMAALLVFELLAEEGSDSFRECQLIELGQ
                     RLLGAKLELLDNLLRDEQGERHE"
     gene            13227..13616
                     /locus_tag="VFR20_00080"
     CDS             13227..13616
                     /locus_tag="VFR20_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0228943.1"
                     /translation="MSNSMNSTAMEPFTAGRRGQLMLHSHDLANWLEIPNTAVVNEIV
                     WWRAFRSPAWAIKNCRNQTRKRVHDFATMTFREDRSYWISQWVASRICRKYRKDLLPG
                     LTAAFISAHDGDMRAQRAFHSGVEVQS"
     gene            13613..>14038
                     /locus_tag="VFR20_00085"
     CDS             13613..>14038
                     /locus_tag="VFR20_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0228944.1"
                     /translation="MIDYTKLHNALAEGGLIVDTITAGRMQRCKVHGDKGGKKSGSYR
                     LFDDELPLCLWWNWKTATFGAWVSDARPQTPADRTRQRELIEQARRERHQQQLAQWAE
                     NRVTLSRLFDAAGAITDACPAGIYLDRRGLSIPNSDALRF"
BASE COUNT         2938 a         4200 c         4387 g         2513 t
ORIGIN      
        1 caacttgccc agtgggccga gaaccgcgtc accctgagcc gtctattcga cgccgccggc
       61 gcgatcaccg acgcctgccc ggcaggcatc tatctggatc ggcgtgggct gtcgattccg
      121 aactcggatg ctctgcgatt catgccgaag ctggactact ggcacgacgg tcaattgctt
      181 ggaacattcc ctgcgatgct ggcggcggtg acttccccat cgggggcgct ggtgagtgtt
      241 caccgcacct acatcacacc ggaaggccac aaggcaccgg tgcccacggt caagaagctg
      301 atggccaccg ctggccccat ggcgggaacc tcaatcaaga tcggtgcgcc tcttgcccgg
      361 cccgatggca ggttgggttt gggtgtcgcg gaaggcatag aaaccgctct ggccgccagc
      421 atcctctctg gtgtagctgt ctggtcctgt gtcagcgccc acggtctgga ggccttcgag
      481 ccgcccggcg acgtgcatta cctgtactgc tttggcgatc acgacgcgtc cggcgtgggt
      541 cagaaagcgg cgtcggacct tgcccaccgc gctgcgcggt ctggcctctc ggcaagaatt
      601 tggataccag aggcaccggg cacggactgg aacgacgaac tgatttctag gagggcttcg
      661 gtatgactga ggcgcaaatc gtggaattga aagccctgga cgttgacgaa ccggaccagg
      721 ccgacccgtt cccgtccatg ggggatcggc cctgctggcg ctgctatgac gaggccatcg
      781 agcgcgacgg caagaaggtt cggcccggcg tccactatca cggcatcaag gagagcaagg
      841 gcgatgcgcc ccccaccatc accgacacct ggcactgtgg gccgttgcac attgaatccc
      901 tgaccgccaa tcaggaggat ggcgatcacg gtctgttgct gcgctttcgc aacgtgaacc
      961 gcaactggaa gaagtgggcc atgccaaagc aaatgctcgc cggtgacggc accgaggtgc
     1021 tttccgtgct ctacgccgat ggcctggaca tcgaccgcaa gagcaaggcc cgtgtgctgg
     1081 actacatcaa cagccagcat cccgggaagc ggctacgggc ggccagcacc accggctggc
     1141 acggcggcgc gttcgtcctg cctgatgagg tgatcggcgc atccgacatt tggtttcagg
     1201 cgaccgagcg cacggcaccc tacggcacgg cgggtacatt cgagggctgg aaagagcagg
     1261 tggcgaccag ggccacggga aaccccatgc tgacccttgg gttatccgcc gcgctggccg
     1321 ggccgctgct ggaaaagctc aatatcggcg gcggtggcct gcacctgttc ggggattcct
     1381 ccaagggcaa gacctcgatc ctcatggccg ccgtgtcggt ctggggcggc ccggccttcc
     1441 tgcgcacctg gcgcacgacc agcaacggcc tggaaggtgc ggcgcgcatg cacacggaaa
     1501 ccctgcttcc cctggacgaa atcaatcagt gcgaaccgaa gcagttgtac gaggcggcct
     1561 atgagctcat caacggccag ggcaagacgc gcgccaacgt caagggtgag gccagacagg
     1621 ccgcccggtg gcgggtgttc gttctctcca acggggaaat gtccataccg gcccgcatcg
     1681 ccgagggcgg catgcgcgtc aaggcgggtc agacgctgcg gatactggat gttcccgcca
     1741 cctcacagac gcacggcgcg ttcgatgacc tgcacggctt cgcatcgggc gcggcgctct
     1801 cggatgcgat ccgcgacggc gcggccaggc actacggtca tgctggccgg gcgttcgtcg
     1861 aggccctggt ggcgcagttt tccgaggggc tggaactgtc cgacgcgatg ggcatcatca
     1921 ccgcgagaat ggcgccggaa ggcggccagg aaacccgagc ggcccgtgta ttcgccctat
     1981 gtggtctggc cggggagatg gcgaccgggg ccggtatcgt gccgtggcgg gaaggccagg
     2041 cgggcgaggc ggccttgctc ggattcaacc tgtggcgcaa ccagcgcggc accggcggca
     2101 ggaacgccga ggacgcggcc attcttcgga tggtggccga cttcatcgac aagcatggtg
     2161 attcccggtt ctctgggctg gacgatcaca atgcgccccc ggtacgggat cgggcagggt
     2221 attggaagga tggcggtggc ggcaggcaat atctactcac ttctggcggc ctgagcgacg
     2281 cgaccaaggg gtatgacatg ggccgcgtca tccgggcatt ggatggcgca caggctttcg
     2341 cacaaaaggg cgcgaacggc aaaaccgccc tggcggtccg cacgcctgat ggcggcgtga
     2401 agaagctcta ccacctggac gccgcgaagc tgcaagaggt ggatcatgaa taaatccatg
     2461 ccctaccacc aaaaaacagg ggttacaggg gttacgaggg ttacagccag tgttcatgcg
     2521 gcctacagcg taaccccagg acagggttac ggggttacag gggttacggc ggcgtggggt
     2581 tcgagcttcg ttgtaacccc tgtaacccca tcaaatcgtg ccggggttac gcggagagcc
     2641 gcatatttat tgggcgcaac ccctgtaacc cgtgtaaccc catcaaaaac gccaaggcac
     2701 acgaaaaaca gggggcggcc atgatgcgac cggaccttct ggcggggctg tacaggtggg
     2761 cggacgcggc caagccggaa ccggccccca atccctgtac gtctgaacag gtgtcgaggt
     2821 taacaaaagt tagcattccg accacgacgg cggtgttgag aaaagttgag gttccggcca
     2881 gcccgatggg cctgcgcaaa gctgcgcata ccccggcccc ggcgaacatg ggaaaacctg
     2941 atgccgatgc cctggccccg gtagccactc aggtagccac ggtatcggtg gtttccactg
     3001 ctgaggttac caagctagtt accacggaaa aagcggtacg cagcacggta cgcacggcaa
     3061 ccgcccccag gccgaacaac ccgaagctac gcgacatcac cgagccggta tcggacgccg
     3121 atcagcgcct ggtcgatatg tcggtgcgcc ggttcgcggg caagggcata ccgatgctga
     3181 aagcccgcaa gctggccgag cggctcttgc gggcactgcg ggaggatgac gaccggcgta
     3241 gctgcgcgga gtgcgaaagc ttccgggccg ggcgctgcgt tcagggcagg caaccattcg
     3301 ggggcggcgg catcgaggtc ttgcaccgct gcaccggatt cagacttgat gggggaaatc
     3361 atgattgacg cattgatcgc tggaaagctt tacggcgtcc cgaagcaggg caccggcaag
     3421 aatggcaaca cctacgccac ggcgaaagtc aaagtagcca ctggcagcgg cgacatgctt
     3481 ctgtgtggcg tcatcgcctt tgacgaaaag gcccaggcgg cgttactggc tctcaccgac
     3541 ggggatagcg tggcgctgtc gggggcgctg acgccgaagg tgtatcaagc ccgtgacggc
     3601 gaacaccggc ccggcctgga tatggtggcc caggttgtca ccaccgcgta ccacgtccgc
     3661 cacaagcgcg ccgccatcga gggcggcgac gcctgaacga cgaaccgaat caacctcacc
     3721 cacaaggaag cgaccatgcg ctactacgaa ctgaacctgt accaacctgg cgtcatcccc
     3781 ggcaccacca agccgttccg cacctggacc acccaccccg gcggacaatt cgaccccggt
     3841 gcgctgatgc tcgaaatgga cctgattgta ttgccctatg ccgtcgcgtc gggcggcagc
     3901 acgatcaccc tgcacggcgt cgccatggaa gacctgaatc agtcgcaaca gttcggcatg
     3961 cacccagacg cccagggcat tgagcaaccc ggcatgacgc tggaattgag gggtggcatg
     4021 aaagccgggc ttccgcttgc caacccggca caggcggatg cgcttatcgt cgggcagatt
     4081 ttccaatcct atggcaactg gcgcggcact gacatgcggc tcgacctcgt ggtgtacccc
     4141 cccggctaca cgatggacaa ccccggaaac ttcgtgttga actggaagcg cggccagaag
     4201 ctctccgatg cgctcggggc gtgtcttgcc gtcgtctacc cggccatgcc gatccgcatc
     4261 aacatcagtg gccggatcgt ggccgaccag gacatgccgc acttctgttc gacgctcgag
     4321 gagctggccc agacggtgaa ggataccacc aagagccatt acctgggcaa gacctatccc
     4381 ggcgtggaca tcgccattca tggcggcgtg atcgacgtgt tcgatgggac cgtgccggcg
     4441 ggtgtcgttc agttgaactt cacggacctg gtgggacaac ccacctgggt ggacaaggcc
     4501 accgtgcaat tcgacacggt gctgcgcgga gacatccaga tcgggagcgt catcaaggcc
     4561 ccccagggca tgaccaatca gcccggcatg gtggtttcct ctcaagcgtc cctacgctcg
     4621 ggggcgaaca atgccctgac cttcgtgggg gatttcaagg tgactggggt acggcacgtg
     4681 ggcaattccc gctctccgaa tgccggtgac tgggcgacgt tcattcaggc cacaccgatc
     4741 atcccgacac caggcacagt caccaacggg gtggcgcgat gacgagtaaa agaccatccc
     4801 ttcgtgaggc gaaagagttt ttgagcgtgc cgaccgaggt tcggctttcg cggttgcggg
     4861 ccgagacgca gcgttgccgg gaacaactca gcgcggcgga cgtggacccc gattcggaac
     4921 tggggcacaa gctcatggcg aaactacagg cgacgggcgg caccctgtgc ctggcccaga
     4981 cgcgggaagg ccacccttgc cgcatgctgg gcgacggcaa gggcgggcgt tgcaagttcc
     5041 atggtggcaa atccaccggg ccgcgcaccg ttgaagggtt gatccggtca gcgtcgaacc
     5101 aggcgaactg gcaccgcaac cgcctgggca tgccgctggt tggcgtgccc ggggtggata
     5161 cggggggggg ggaatcacgc cagaagtagc atggccccag ccaaaaaaaa ccatggggtt
     5221 tgttgctgtt tttggaacag tacgtgcgcg cgcgcgcgag gctggcttcc cgagacccca
     5281 gaatcggtgt tctcgacgct acggcgaact gttgaagatg ctggctaggg catgatcgga
     5341 ttcaggccgt gggttgcagg ctaggttgca ggttgccacc caaaaaaaag agcctgcaac
     5401 ctggaacgtg ctgtagatac acttcggcgt gatgtgcgca cttatccgcg tcgttattgc
     5461 ggcgtccatt tcgtggcccg gcccgtgctc attggcctgg cgggtgaaat gccgcgacgg
     5521 tatttgcgaa gttatccgcg cacttatttg tacacatttc cgcgccatcg gcttttgccg
     5581 ttcggcgaga tggcccggtt ccagccacat caagcggcgc aagcagatat gggaggcgct
     5641 gcatccgggc gttgagggtg atgcactgat ctgtgcactg gatttcggtc tttttttaac
     5701 aactgcctgc gcatggcggt tttcagcgaa aacaaccatt tgtggtggca ttaatgtttg
     5761 ttggtcattc ttaatgtaag cacaaaatgt ttaggttcga gcgcccccgg cctcgacata
     5821 tccagtagcc ttgctggaac gcatgaaaag gcattgtgag tgatgcggtg tcaaccagag
     5881 tgatccccgt tgcttcggcg gcaatatgca tgatccggca attcgcggca atccgagtgc
     5941 cgggaaaaca cttcacgggg gtggcgggtt ttcagcgcgg ggtgcctcca caaaattgcg
     6001 gaggcgtcca tccggtgggt cttgcagtcg ggagcgagac cgtaccgacc gcaaattagg
     6061 tccgttgccg acttcctggg gcgggcgggt taaagttccc gacgggaacg ctaggcaggt
     6121 tatcgccgat cttcgccaat tcgccgcatt cgatatgtcc acatgctgcg ctgtggataa
     6181 ctgcccagca acggtgccaa gatggtattt ttgatggtat tttttttgat cgccagtcag
     6241 aaacacgtat tgatgcggct ttccaggcac agtttggagg atgtagcacc caccaaactt
     6301 aagtctacag cagtctacaa cagtacacga aacccgcatg cttattggcc ttgcgggttt
     6361 tttgttgtct acagccgtct atgtcagtct atggacatgg atggtatcgt gatggtattt
     6421 tggatggtat caacctgccg ccgaaaataa ataccatcat gggcttatca gacaccgctt
     6481 gtagaaccgc caaggtcaaa gacagcgaca ttaccaaaga cgggaaaaag aaaagctcta
     6541 tcaagctgcc ggacggtaca ggcttgtatc tgatggtcaa tcgtgcaggt aagaagtatt
     6601 ggcgctacaa ctatcggttt ggtggcaaga gcaaaactat tgcctatggc agatacccaa
     6661 gcatgacact ggcggcggcg cgtgatgctc atgcagcagc gcaaagagag ctaagcgccg
     6721 ggcgtgaccc aatgacggtc aaaaagagcg tcaagcaagc ggcgctgctg gcggcggaaa
     6781 actccttcaa gtccgtcgcg caaaagtggc tcgcccagaa ggcccccacc tggtcggcca
     6841 cccacgagga aaaaacccgc cagcttctgg aacgcgatgt ttaccccgtg ttcggggccg
     6901 agcccataca ttccatcacg gcggcgcaag tgctcgccat ggccagaggg atcgaggcgc
     6961 gcggcaacgt ggaaatgcca aggcgctcaa tctcggctgt tggccaggtg ttgcgctacg
     7021 ccatcgggtt ggggctgatc cagaatgatc catcccaggg gatggccggc cagctaaacg
     7081 accgcccccc ggtacagcac tatgcccatg tgggcaccaa agagctaccg ggcctgctgc
     7141 gggcactgga tgcattgcca gtggacccca ctgtgctggc ggtcaggctg gacatgctga
     7201 cgttcgtccg tggtggcgaa ctgagaaacg ccgaatggtc agagttcgac atcgaggcaa
     7261 aggtatggcg tgttccaccc ggcaggatga aggggcgcaa gtgggccaag gagaacggcg
     7321 aggcgcatat cgtcccgctc gccccccagg cggtggcgat tctgaaagag ctgggggcgt
     7381 tcaccggaca ctacgcgctg ctgtttcctg ggcgcgacga ccctacccag ggcatcacgg
     7441 gggcggccat ggccaaggtc tgggcacggc tcggcctgcg tggccagcaa accacccacg
     7501 gcctgcgcgg tctggcatcc acccaggcca acggcgcgcg ccgcccggac ggatcacggc
     7561 ttttcgatga ccgtctgatc gaggcccagc tatcccacaa gatgagggac cgcatcaagg
     7621 gttcatacga ccatgccgag catatcgagg cgcgcacccg gctgatgctc tggtgggccg
     7681 actacctcga cacgctcagg gcgggcggca acgtcatacc gctgacacgg caggcatagt
     7741 cccttgtctt gcatgtcggt atccgtatgg atacaataat tcatgcccca cctgatccag
     7801 caaacagaat cgttcgcggc atggcatacc ggcctgcgag acctgcgcgc caaggtggcg
     7861 attgcccgac gcattgagcg cgtggccgcc ggaaccttgg gtgatgtgaa atcgttgggc
     7921 aatgatttat ctgagctgcg cgtggatata ggccctggct accgggtgta tttcacaatg
     7981 cgcggtggcg tgctggtgat cctgctggcc gggggcgaca aacgcaccca ggccgccgac
     8041 atcaagcagg cgagaaaact agccatggag atagacccat gaaagcgaaa tccaaactga
     8101 tcccattcga catgaccgcg catctggaca gcccagaggc catagcggaa tacctgaccc
     8161 aggtactggc cgattgtgac gccgacgaat tgctgcgggc gctggggcat atcgccaagg
     8221 cgcggggtat ggcccaggtg gcggaagctt cgggcctggg gcgtgaaagt ctgtacaagg
     8281 cgttggcccc tggtgcgaag ccgcgctatg acacgatcct gaaagtaacc cgcgccctgg
     8341 gtgttcgcct gaccgcgcaa atcgttgaaa atcccgcccc cgtcaaaccg aagcaggcca
     8401 ctcacgcggc ggtggcggcc tgatccttta cgccagcacc tagcccgacg gggcgaaaag
     8461 ccggaaacct tcaccggcct ggtgctggct ccctcattga aggttgcaca gtgaaggtgt
     8521 gcgcatggtg aagcccagga aattgaccct acggcaggcg gtcaaagaaa caaaaatgcc
     8581 cgtcatgcgg ttgggcgatc ttcttgtcta tcagttcgga ggggtgattg tcggtgcgtt
     8641 tatgccaaat gccactgatt ggtcaattca tgactttggg caacccgaga cagagaacta
     8701 catccggcag ctatatgccg atgatggctg gattgaatcc atcacgcact tcgacgacga
     8761 ggcattggca ctgcaccaca attacacgtt ggggttgtgg gttggcagaa aattctctgg
     8821 cagtggcggc gatgctcaac gttttgaggt tgactgcgcc caggccaccg agttgctagg
     8881 gccaaaccct tcccgtgaaa tggtgctgtc cgcgatcaga atcgtactga tgatgcacaa
     8941 gaagtacctg ctatcggctc gaaccggggc gaggttcgag gcaggccgca agccgaacac
     9001 agagggacca atcagcaaag ccatgggctc ggtgataaag agaaatccaa ccataaaaaa
     9061 tgccgacctg tggaacgcga tgaagtccag ccccccgagg ggttggacct tctacgacaa
     9121 cgaccaaggg aaatacattg aaggcccgcg agggggccag aacatgggct atgaccggtt
     9181 ccgcaacatc gccgcacaga aacggaaagc gcaaaagtag cgaaccacgt aaggaatttc
     9241 acgggttagc gaactcgtga aactggatgt tcagaattgc tccacacatg gcgcttgata
     9301 tggcgtcact tttaatggag tggttctatg acacatcatg tcgcacccgc cgatgatcga
     9361 ctcatcactg accgcgaagt cgcccacctt ctgggctgtt cccgttcctg gccctggaaa
     9421 ctgagccgcg acgggaagtt tcccaaaccc atccagctgt ctccccgctg taccagatgg
     9481 tacttgtccg ccgtgcgcac ctatatggct gaccctcaag gttggatggc tgaacacacc
     9541 tcaaaagtgg aggtgtgaac catggcgacg accaaacaaa aggaccgcat ccctgtgaaa
     9601 gatgcgaccc tgacgaccag cttgaatcat accccaaacc tcgttcactt gcgcaaaggc
     9661 gttcccatta ccgacagcct ggccattgcc cgcgagttcg gacgtagcca taaaaacgtg
     9721 ttgcgcacat tggatgacct gatcggcgac ggcacaatca gccggctcaa ttttgagccg
     9781 gcggaatacg ttgatgagca gggaaaaccg cgccgcatga tcgaactgga cgaccgcgcc
     9841 gcgctggttg caatgccgtt catcggtggc cgcaaatccc gcctggggca agtccatttg
     9901 gtgaatgcct tcctggcaca gcgcgatttg ctgtccaaga cgtggacgcc acagatcatc
     9961 gcggcccgtc gtgaagcggc gaccggctac cgcaccatgt cggcggtcct gcaaatgcgc
    10021 cgcacggagg acggaaagga aacaccccgc catgtctaca gcaacgaagc gcgtctactg
    10081 aacttcgcac tgtccgggag cttcgaggcc atggatcggg agactttgag cgagcaggac
    10141 cttcgcgcgt tgaccagcct ggaagtacgc aactccgcga tgatcgccat gggcaggccc
    10201 tacgccgaac gcaagttcga cttgatgcgc tacgccgagg gcttgcggat tccgcgcctg
    10261 acctcttcgg ctacggcgtc catcggaatg gggggcactc atgtctaaga aatccacgtc
    10321 cggcgaagcc cagcgccaac gcatccttga ggcgctcgat cccaacccca agaccagtta
    10381 cgacctgcgc cgcatgggtt gctaccagtg cccgacccgc atctatgagc tgcgccgcat
    10441 gggctacgac atccgcaccc agcgcgtgag tatttgggac gaggacgggt atcagcacca
    10501 cggtatcgcc ttatacgcct tggtcaaggg gaaaaaatga ctaccaaaac cattcataaa
    10561 atcgaagtcg attcgtctca gttcaaggcg ttctacgact tgttcaagca gtattcggcg
    10621 gccctggatg acatgcccga ggagtggaag agggtcaatg gtgaggtgag cgacgcgggt
    10681 aagtccatgg aggatttcgc cacggcaagc ggggtatcca aggaatcgct tgccatggcc
    10741 gccgcgcaag caaagatcat ttccgatgcc gtggcgggag tcgccaaggg cacccacgcc
    10801 ggcaccaagg cccagaagga ttttctggta tcgcttcacg gcagcggaaa ggccatgcgg
    10861 gagatggcca aggatgccaa gaagctgggt tccgaggtat tcgggatcgg caggtatttg
    10921 ttgaagatcg gcgtcctggg ttctggtgtg gcgggtctgg gcggcattct ctctggcctg
    10981 ggtctggctg atttagcccg ttccgccgtc gccggccagc gcgatgcgcg cggaatcggc
    11041 gtatcggtgg gtcagttgca ggctttccgt accgacttcg ggcgctatgc cgatcccggc
    11101 gtgcttaacc gcatggccga tgcgcagggt gacatggcca agtgggggta tctgtcgctt
    11161 gccacgggta ccccgctggg tcagattccc ggtgcgaacc cggtggccct gtcgctcgcc
    11221 atgatgcgcc gcgcacacga ctggtgggac aagacgcccg tgagccaacg cacggccaat
    11281 aacctccaga tcaccggcct gaatcaattc atgtcgttcg aggaggttcg ccgcctggga
    11341 gcgatgacga acggggagtt cgcccaggcg cgcaaccgct tcgccagcga tgcgcgcaac
    11401 ctgaacctat ccgggcgcga ggtggacgcc tggaacacgt tcattgtcga ggtgaagaac
    11461 gcgggcatgg cgctcgatac cgacctcaag cgcaagctcg ccgagctggg gccgagcatg
    11521 ggcgggctta tcgagacatt gagcaccgat ggccgccagc ttatcgacga ggtgctgacg
    11581 ccggcgaaca tcaacaatct gaagagggac atcgacggct tcaccaatct gctgggttca
    11641 cccgagttcc acaatgacat acgcgagttc atccagttcg tgggggatgc cgcgcacggc
    11701 ttgaaggtgc tggccgagtg gctcgacccg tcgctgaaat cgagcggcaa tcagttgtca
    11761 gtccctggca ccggcgcggc gatcaatggg ctggaccccg gctcatcaac cgggcattcc
    11821 atctggcacg acctggccag ccccggcgac atggtcaatg atggcttgtc gcagttgggg
    11881 aggaaagcca aagcccttga ggggaaatcc cgcgacaagg cgtatgacga cgcctctctc
    11941 tggggcaagc tgaacatgga cttcggcggc aggaaatcca cccctacccc agcccaggta
    12001 ccgaaaaagg agctgtacct ctacaacctg gaaaagaagt atggcttgcc ccacggcatt
    12061 cttgattcga cgtggtttgt cgagtcatca cgtggggtga acgccgggcc gtcgggttcc
    12121 ggcgcgctgg gtgatttcca gttcctcaag gccacgggta ggcaatatgg cttgatcaac
    12181 gacaccgaca gaacggactt ccacaagtcg gcggcggcgg cggcgagcta ctacgccgac
    12241 ctcaagcgca agttccatgg cgacatgaaa aaggccatcg ccgcgtactt cgacgggccg
    12301 gccaacgtgg cgggcaacta tgcgaaacag ggcgggaaga tgccccccca gacacagcac
    12361 tacgtcgata agactctcag cacctggcgc gcgaaccgcg aaacgatcat caaggtggtt
    12421 gtgaccaaca gcaccgggtc caatgtcgcg gtatcgacca acgcgggggc gctgtcatga
    12481 gccttcacca catcaagacc gttctcgacc gcatggagcg cccccggctt gcacaacagg
    12541 gtgcattcga cgacaataac cccgtcggca aaacaacgct gaccgggttt ggcgacctgt
    12601 gtagctcatg gcgaatgaac cgccgccccc tggcggtttt tcattcgcaa gccatgtctc
    12661 ctttgggcgg cgatggcggg aataccttcg ggtatgccgg tactcatgag cgccggttcg
    12721 ccaatctcgc cattcgctgc ccaccatcgt ttggcgacga tgcgggcggt tcaaccgctc
    12781 atggaggccc agacatggcc gcactaccca catccgcgca caccgcgcac tttcccctga
    12841 ctgaaaaatc cgcccgccgc gccgcacggc aatggttcac tggcacgcct accgccaccc
    12901 tggcgacttg gtgtgatgac cgctgccgcg cccactgcga cagcctgccc gaaaacccgg
    12961 cccgcaccca ggcattccac gacgcctttg ccgccgagat cggttccatc atcgcggggg
    13021 gggttccatc atgaatgacc acacaaccat gcgccttgag gatcaccgcg ccgacatcat
    13081 ggccgcgctg ctggtgttcg agttgctcgc cgaggaaggc tccgacagct tccgggaatg
    13141 ccaactgatc gaactcggcc agcggctttt gggagccaag ttggagctgc tcgacaacct
    13201 gctgcgcgac gaacaagggg aacgccatga gtaactcaat gaacagcacg gccatggagc
    13261 cgttcacggc gggcaggcgc gggcaattga tgctgcacag ccacgacctg gcgaactggc
    13321 ttgaaatccc gaataccgcc gtggtcaacg aaatcgtctg gtggcgcgct ttccgctccc
    13381 cggcatgggc catcaagaat tgccggaacc agacacgcaa gagggttcac gacttcgcca
    13441 cgatgacatt ccgggaggat cgttcgtact ggatcagcca atgggtggcg tcccggattt
    13501 gccgcaagta ccgcaaggac ttgttgccgg gcctgactgc ggcgtttatt tccgcacacg
    13561 atggggatat gcgcgctcag agggcctttc acagcggcgt ggaggtgcag tcatgatcga
    13621 ctacaccaaa cttcacaacg cgcttgccga gggcggcctg atcgtggaca ccatcacggc
    13681 gggccgcatg caacgctgca aggtgcatgg cgacaaggga ggcaagaaat ccggctcgta
    13741 ccgcctgttc gacgatgaat tgcccctgtg cctgtggtgg aactggaaga cggcgacttt
    13801 cggggcttgg gtatccgatg cgcgccccca gacgccggcg gatcgcaccc gccaacggga
    13861 gctgatcgag caggcccgac gggagcgtca tcaacagcaa cttgcccagt gggccgagaa
    13921 ccgcgtcacc ctgagccgtc tattcgacgc cgccggcgcg atcaccgacg cctgcccggc
    13981 aggcatctat ctggatcggc gtgggctgtc gattccgaac tcggatgctc tgcgattc
//
LOCUS       DASTNK010000002        34741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_187470, whole genome shotgun sequence.
ACCESSION   DASTNK010000002 DASTNK010000000
VERSION     DASTNK010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 34741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34741
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187470"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /gene="mutS"
                     /locus_tag="VFR20_00090"
     CDS             <1..1359
                     /gene="mutS"
                     /locus_tag="VFR20_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU0228945.1"
                     /translation="GYDEELDTLRQLAGDNGAFLMEMETRERERTGIANLRVEYNRVH
                     GFFIEVTRGQADKVPDDYRRRQTLKNVERYITPELKSWEDKVLSARERGLAREKLLFD
                     QMLAQAAGHAAALAASAAALARIDALAALAHHARRHAWVAPELCDDNCLDIQAGRHPV
                     VEHSIETFTPNDCVMHEERRMLLITGPNMGGKSTYMRQVALIALLARMGSFVPATRAR
                     IGRLDRIFTRIGAADDLAGGRSTFMMEMTEAAAILSAATAQSLVLMDEIGRGTSTYDG
                     LALAWSIAHRLLTHNRALTLFATHYFEITRLPAGTQGAVNVHVAATESASGIVFLHEV
                     RPGPASRSYGIQVAQRAGIPPAVIRLASRELARLEARGAPTPQLDLFSASTPTADEAD
                     DGAENDMTTRIEAADFHEIAGDASDERALALQADLADINPDSLSPREALDILYRLRAD
                     YL"
     gene            1390..2574
                     /locus_tag="VFR20_00095"
     CDS             1390..2574
                     /locus_tag="VFR20_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0228946.1"
                     /translation="MNPRTPLPILGGLSPQAFMRDHWQRKPLLIRQAIPGFRPPLTIP
                     EIRQLARRDEVQSRLIWRDEAGWHMKHGPFSRLPAPHRPGWTLLAQSVDQHDDETARL
                     LHQFRFIADARLDDAMISIATDGGGVGPHFDSYDVFLLQAHGRRRWRIGAQKDLSLRP
                     GLPLKILQDFRPDQEYVLEPGDMLYLPPHLAHDGVALGECMTISIGFRAPTLAVLARG
                     LLDAAGDQVTARAGGDTGLYADPPLPGPALARVFADAGEPATPTPARLPDSLIQASLN
                     AVAQMRFDEKLAARFLGQWLTEPSPAAVFEPEPKPLHLSHGLPATGRLCLDRCTRLMY
                     RSRELYINGEVADLPASKALRTLADRRELACASTAARSLTAPERSMLEGWLEEGWLHC
                     HD"
     gene            2757..4550
                     /locus_tag="VFR20_00100"
     CDS             2757..4550
                     /locus_tag="VFR20_00100"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEU0228947.1"
                     /translation="MLAPLSLPESKQTLLGELTQGLEPDALNWLSGYFAGIAAAARPE
                     RGVAPVVAAVPSAPAMRPLTILYGSQTGNAQRTAKALAEHLEKLGLPTRLVRADRYAT
                     KELKDEQLLYVVISTQGDGDPPDDSLAFVEFLNSRRAPRLPGLKYAVLGLGDSSYPVF
                     CGTALALDARLAELGAQRLFDAGTADLDVETVATPWRDKALEQAQTVLGQSEAPRASI
                     TPLRPKATRISRDNPFQAELLASQPITAPGSIKDVLHLEISLSGSQLSYQPGDALGVW
                     PTQAETLVDNVVSTLGLDANEAVEVGGVSRPLREWLSHHRELTRLTRPFLIAHAKLSG
                     SDELGRLLDDDARDALRHTLETRQLIDILRQYPAPWTAQGVVQALRPLAPRMYSIASS
                     QASVEDEVHLTLANVAYDHEGEARWGVASNYLSQLDEGARLPIFIEENTRFRLPTDPA
                     RDIIMIGPGTGVAPFRAFVQDRAARAAPGRNWLFFGNPYFHTDFLYQTEWQGALADGS
                     LHRLDLAFSRDQEEKIYVQDRLLEHGADIYQWIQGGAHVYVCGDATRMAVDVQQTLLR
                     IASREGGLDESGARQWLDDLAAQGRYARDVY"
     gene            4621..6339
                     /gene="cysI"
                     /locus_tag="VFR20_00105"
     CDS             4621..6339
                     /gene="cysI"
                     /locus_tag="VFR20_00105"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEU0228948.1"
                     /translation="MSDTPLSHLEHVKQDSRYLRGTIEQGLNDAVTGAISDDDNKLLK
                     FHGSYQQDDRDIRDDRRRRKLEPAYAFMVRARLPGGILTPSQWLAFDHIATTWATRGL
                     RITTRQTFQLHGILKRHLKPVMQAIHDAMATTIAACGDVNRQVVSSVNPLLSDQHQIV
                     QDWTEKLSAHFLPRTRAYSEIWLDGKRVDTEPSDPFEVEPIYGAAYLPRKFKIGIAIP
                     PTNDVDVFSHDLGLIAIIENGVLRGFNVAVGGGMGATHGDATTYPRLGSLIGFVSPDK
                     VISLAEGVVTVQRDFGNRSERQHARLKYTVDRMGLDAFTAELEQRLGYALEPMRPYRF
                     DSNGDRFGWIKGENDTWHLGLYVESGRLWDTDSLKLLTGMREIARVHKGLFRLTCNQN
                     LIIAGVADADKAGIDALVREYGLDDYTRSPEVHRQAIACVALPTCGLAMAESERYAPV
                     LLGKVHGLLDKHSLGDAPILLRISGCPNGCSRPYLGEIALVGRAIGRYDLRLGANFTG
                     ERLNVPYRENITEPEILDALDALFASYADQRHDAEKFGDFLVRSGVIPAPARNIIPII
                     HKPLRT"
     gene            6336..7814
                     /gene="cysG"
                     /locus_tag="VFR20_00110"
     CDS             6336..7814
                     /gene="cysG"
                     /locus_tag="VFR20_00110"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HEU0228949.1"
                     /translation="MNLFPLFLSLTGKRVLVVGGGPIAERKTRLLLDAGARVTVGTLT
                     ACEELTSLSAQGHVNLVIGAFEEHWLDPVWFVVVATGDHALNQEIARRCEQRRLFVNV
                     VDDAEHSSAQVPAIVDRSPVTIAISSGGHAPMLARRVRERLETLLDGSLGPLATLAGK
                     YRRAIQRHFPDMRKRRGFYDWLAEGPVHQALAANLPDAAEHALEHGLMHHSDTSEGMV
                     ALVGAGPGDAGLLTLNALRALNRADVILYDRLVSPDVLALARRDATRIEVGKRPGEDH
                     DATQRGIHELLLRHARLGHYVVRLKGGDSLVFGRGGEELEYLRQHRVRYQVVPGVTAA
                     LACAAYSGVPLTHRDHAQSVRLVTAHGRDGHDEPDWRDLARPGQTLAFYMGVSRLPTL
                     AANLMAHGRSADTPFALVENGTRADQRVLTGTLGRLAELAARHQFKAPALLLLGEVAA
                     LADTLHWYGTRVETDAPGHAGIHDSRTDAIIGESLGETLLPA"
     gene            7860..8975
                     /locus_tag="VFR20_00115"
     CDS             7860..8975
                     /locus_tag="VFR20_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HEU0228950.1"
                     /translation="MATTDFDDDDVFSDKPERFPRFGWEALGSMSPAYFGMVMATGVV
                     SIAANLSGHDTIARALFILNIVFYVVLLVLSGLRTVFALRRVLFDLTHHRRAPSFFTS
                     VAGTSIVGIQCIEFAHRPDIAIGLWILAIIMWLGLTYSIFTALIIKTNKPTLDHGING
                     SWLLAVVATQSIAVLGSILTANHALPDRLEMNFFALSIWLWGGMLYIWMISLIFYRDV
                     FFRFSPHDLAPPYWINMGAMAISTLAGSLLILNAGDAPYLKSLLPFLKGFTIFYWATG
                     VWWIPMLIVLELWCYVFKKFPVRYDPLYWGAVFPLGMYAAGTYTMDKAMHFGFLEPLP
                     TAFFYIALVAWAATFVGLARQLWDRAMHTWRPGAARG"
     gene            complement(9095..10234)
                     /gene="bamC"
                     /locus_tag="VFR20_00120"
     CDS             complement(9095..10234)
                     /gene="bamC"
                     /locus_tag="VFR20_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEU0228951.1"
                     /translation="MRLKKHATAVVVGLSLLALSGCSLNKFMSDQESVNYKSTVAGEP
                     LTIPPDLTQADSNPHYRAPEGMATYSKYAQSQAARNAQNADNAVLPNTPGMKVMRDGD
                     IHWLVVDQPAQSIYPKVVEFWGDQGFTIHNQDPKAGLIQTDWAENRAKIPEGWIRSAL
                     GGLLDQVFDSGERDRFRTRIERVNGHTEIYISHQHMVEKVSPDFSTSKWVPGKEDQGL
                     NAAMLARLMVYLGTNVNEAHTLIAQAVKDPTQTKVQAQTAGQSADLTVSEPFDRAWRR
                     VGIALDSAGFSVDDRDRSAGDYYISYLDTDTGVKIEQQTFIGRLFGTKNTAAPTKYRL
                     HVAQQSDGTSSLVTVLDANGKRDDSATAKRIITVLSQHMGTPSGS"
     gene            complement(10246..11148)
                     /gene="dapA"
                     /locus_tag="VFR20_00125"
     CDS             complement(10246..11148)
                     /gene="dapA"
                     /locus_tag="VFR20_00125"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU0228952.1"
                     /translation="MASTDSPIPRFQGSMVALVTPMHPDGSLDFNAYRKLIDWHIEQG
                     TDVLAVVGTSGESPTVSVDEQAELIRIAVEHSAGRLPVIAGVGGNSTSEAIHLSRHAK
                     AVGADAGLSVVPYYNKPTQEGMYQHFKTVAEAVDLPTVLYNVPGRTSADMSNETLARL
                     ARVPGIVGVKDATGNIARVADLLRRVPKEFMLISGDDPTAAALILLGGCGNISVTANV
                     APKLMHELCAAALAGDAVRVRELNGRLALLNQALFVESNPIPVKWAVAELGLTELGYR
                     LPLTPLHEQYRAVVRDAMKDAGLL"
     gene            complement(11290..11940)
                     /locus_tag="VFR20_00130"
     CDS             complement(11290..11940)
                     /locus_tag="VFR20_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU0228953.1"
                     /translation="MDSLIQTITIYALPVLFGITLHEAAHGYVARLFGDPTAYQAGRV
                     SINPMRHIDPMGTIVVPLVLLFGSKLLGMGGILFGWAKPVPVDWSRLRQPKRDMLWVA
                     LAGPASNLLMAVLWALSLRMLASTGAGQADFWVQMAIAGIQVNLILMALNLIPLPPLD
                     GGRIVFSLLPSRMAWRYSKIEPYGLVILIVLMLAGGLWFVLTPFLAFGQAVVRWFL"
     gene            complement(12126..12755)
                     /locus_tag="VFR20_00135"
     CDS             complement(12126..12755)
                     /locus_tag="VFR20_00135"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEU0228954.1"
                     /translation="MAQLFVVHSQNPQARLLRQAGQLLAGGGLVAVPTDSSYAVLARL
                     DDKAAADALRRLRGLDERHHLTLLCRDLAEIGHFARVDNAQYRLLKKATPGPWTFILE
                     ATKEVPRRVSHPSRKTIGIRVPDHRIALELIEHAGSPLMSTTLIPEDEDEPLNDAEDI
                     YQRYGNQLAAVIDGGACPLMPTTVLDLTGPVPQVIRRGQGNLAGLGIEA"
     gene            complement(12839..13294)
                     /locus_tag="VFR20_00140"
     CDS             complement(12839..13294)
                     /locus_tag="VFR20_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEU0228955.1"
                     /translation="MALIVILHMLSAVIWVGGMFFAYLALRPAADAVLEPQQKLRLWA
                     VVCARFFAWVWLAVVVLLVTGVWAVFGKFGGMANAGGLIHVMMGLGGIMILIFLHVYF
                     APFRRLKQAVAANDWELAAGKANQIRMLIAINLVLGLIVVCVAAVSGYH"
     gene            complement(13590..13823)
                     /locus_tag="VFR20_00145"
     CDS             complement(13590..13823)
                     /locus_tag="VFR20_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0228956.1"
                     /translation="MKQLYKTLLVSALLAAPFATVQAHETQAQHEAWIKAQKIDVQPT
                     LTIKETTIPASSVNESNSSSSSSMDKMNSSHKK"
     gene            complement(14085..14160)
                     /locus_tag="VFR20_00150"
     tRNA            complement(14085..14160)
                     /locus_tag="VFR20_00150"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14125..14127),aa:His,seq:gtg)
     gene            14504..15637
                     /gene="nadA"
                     /locus_tag="VFR20_00155"
     CDS             14504..15637
                     /gene="nadA"
                     /locus_tag="VFR20_00155"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEU0228957.1"
                     /translation="MSNIKAVEFERPSPVPGARVTAEAWARVPAPVSSDQKSALKNRI
                     KELLRAQRAVLVAHYYVDADIQDLAEETGGCVSDSLEMARFGRDNPAQTLVVAGVRFM
                     GETAKILSPHKRILMPDLDATCSLDLGCDPVEFGAFCDANPDRTVVVYANTSAAVKAR
                     ADWMVTSSIGLDIVAHLHERGDKILWAPDRHLGHYIQKQTGADMLLWQGSCLVHNEFK
                     GLELEALRREYPGAKVLVHPESPDAVIAQADATGSTSQLIAAAQSMDADVFIVATDQG
                     LLHKMRQAAPGKLFIAAPTAGNSATCKSCAHCPWMAMNSLQGLAHVLETGVNEITLDP
                     EIGRRALGCIDRMLAFAAQRKANVRPGPDLAQEQELFAGVGPA"
     gene            15634..16515
                     /gene="nadC"
                     /locus_tag="VFR20_00160"
     CDS             15634..16515
                     /gene="nadC"
                     /locus_tag="VFR20_00160"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU0228958.1"
                     /translation="MNATNTSPQGFMPFDNSLRAAFEAAVESALSEDVGSGDVTGLLV
                     PATATATAELKVREEAVLCGAPWFEAVMRRCAPQAQVEWRYAEGDLMAANSLVCRVRA
                     PARALLTAERTALNFLQLLSATATATRHYAAIVEGTNAQILDTRKTIPGLRLAQKYAV
                     RVGGGANQRLALYDGVLIKENHIAAAGSIKAVMEAAASLPRTVPVQIEVESLDQLRQA
                     LEAGATSVLLDNFTLDAMREAVVLTAGRALLEASGGINHDTVRAIAETGVDRISIGSL
                     TKDIRAVDYSLRVTQMG"
     gene            complement(16520..18169)
                     /gene="nadB"
                     /locus_tag="VFR20_00165"
     CDS             complement(16520..18169)
                     /gene="nadB"
                     /locus_tag="VFR20_00165"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEU0228959.1"
                     /translation="MNRFDVAIVGSGLAGLTVALHLARDKKVVVIAKRTLLDGASDWA
                     QGGIAAVLDSHDSHDQHINDTLTAGAGLCDEVVTRQIVENGKAAIRWLIDQGVPFTPD
                     DTAELGFHLTREGGHSQRRIIHAADATGHHVQVTLEQKVRAHPNITLLENHFAVELIT
                     RGKRGRPAGVPGADDNAGDLARCLGLYVLDEANDRVETILAAHTVLATGGAGKVYLHT
                     SNPDTSTGDGIAMAWRAGCRVSNMEFMQFHPTCLYHPSAKSFLISEAVRGEGGILRIP
                     GGAEVDAGLRFMSRHDERLELAPRDIVARAIDFEIKKRGLNYVELDITHRPPEFIEEH
                     FPTIRARCQALGIDITRQPIPVVPAMHFTCGGIVTDLQGRTDIPNLYAVGETACTGLH
                     GANRLASNSLLECLVVGRNVARFIESQEAGPLPPDRGVPDWDDSRVVDEDEAVVISHA
                     WDEVRRLMWNYVGIVRTGKRLARARHRVDLLLEEIAEYYRAYRVSGNMLECRNLIQVA
                     ALVVESAQSRKESRGLHYCQDYPRTLPRALPTVLTPGLRVG"
     gene            18541..19983
                     /locus_tag="VFR20_00170"
     CDS             18541..19983
                     /locus_tag="VFR20_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEU0228960.1"
                     /translation="MLMVLTHWPHIVFALSVVAGAAAAIHAAMTKNDVRSAIGWVGVI
                     LMSPLFGPFIYLIAGINRVRRDQISQQRDRAMKDYTRIDTPAVHDVTPYSSPQFASLR
                     ILGDRVSRFPLHDGNHIQILAGGDEAYPAMIAAIDAARETIGLQTYIFDDDPEGRKFV
                     EALARAQSRGVEIRVLIDSIGSKYSRPPVIHRLHRHGIRTALFMTNPLGMKMPYANLR
                     SHRKILVVDGRIGFTGGMNIRAGFVSAVARDKTAMDTHFRLEGPVVLQLVSVFAHDWE
                     FTTHETLPMPVWCHDAWTPPEPRVPARCVRSGPDRAIVSTHNMLLGAFAVAQRHIRIQ
                     SPYFLPDQILLGAINTAARRGIRVDVVIPGANNLRLVNYAMTAQLDQIIRNGCHVWRA
                     AGNFNHSKLITIDGAWSYVGSSNLDPRSLRLNFELDVEIYSRHMAADIERLIDAEIQG
                     AQAVTLENLAKTPFRKRLRNKIIWLASPYL"
     gene            complement(20104..20295)
                     /locus_tag="VFR20_00175"
     CDS             complement(20104..20295)
                     /locus_tag="VFR20_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0228961.1"
                     /translation="MTKQSRLALALSSMVLSLGLVAAPAAFAAGTTTTAKHHKTHKVH
                     KASKHHKAHKKAAPKAKAK"
     gene            20792..22000
                     /locus_tag="VFR20_00180"
     CDS             20792..22000
                     /locus_tag="VFR20_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0228962.1"
                     /translation="MKAVTPDGSDKATTWRDETRVLHGDQGMDPGSAVVAPIHYSATF
                     RAETPAEFAEMANTPRPRAYYTRYGNPVHKRVEKLIADLEGTETAMVLGSGMGAISTT
                     VLALVGRGDHVIAQRRHYMSTSRLFEEVLPRFGVETTLVDQTDVSAFAKAMRPETRLV
                     MLETPVNPTLSLTDLGAVAALARPRGVLTIADNTFASPLNQQPHALGIDIVVHSATKY
                     LGGHHDLTAGAVCCTNELAERIWDMHTTLGSVLSPMDAWLLLRGLRTLPLRVERINAN
                     ALGLAQWLERQPQVERVFYPGLASHPQHDLARNQMSGFGAVVAFSIRGGYAATSRFVS
                     ALKLATHAVSLGGVETLIVHTAAMWAGTMTEAQMASAGIEPNFVRMSVGVEHIDDLKA
                     DLGNALMQVD"
     gene            complement(22077..23255)
                     /locus_tag="VFR20_00185"
     CDS             complement(22077..23255)
                     /locus_tag="VFR20_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0228963.1"
                     /translation="MGKVLAITLTIQSLVSAAALTVPVLAPALAPVFGVPGELVGVYV
                     ALIYVGAMFSSLAAGGYVARFGAIRISQGSLLMCALGLACSLTAVPVLAAAGALLIGC
                     GYGQVTPASSHLLVKTTPTHRMSLVFSIKQTGVPLGGVLAGLFLPRLDAWTGWRGAVL
                     VAIIACIVSAVGVQFSRKDLDADRTAGSPLGLGNLREPVRVIIRHSALRLLSLGSLTF
                     SAVQLCFTAYLVTFLHDSLGYTLIAAGVMMSVSQVAGVVGRVLWGWCADRFVGAIAML
                     TVLGVAMAVFCWVLAGLVVGTPPALLVVVLIALGSAALGWNGVYLAEVARQAPPGQTS
                     IATGGTLAATFLGVVVGPPIFAGIASLANSYRPAFMLIAIPALIMGLVFAWRRKNFAA
                     "
     gene            23631..24824
                     /locus_tag="VFR20_00190"
     CDS             23631..24824
                     /locus_tag="VFR20_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0228964.1"
                     /translation="MSNTPFSFKKIAVPAFGPSLLFGTGKGAILPVIALTARDMNASV
                     ALASLIVGLIGIGSLVSNIPAAMIIARHGERVSMIGAAAVSIVGLILCLLPSTVWLLA
                     LGVLLIGMAQSVFFLARQTYLIEAVPVHLRARAMSTLGGTMRIGVFVGPFLGAALMHF
                     MGIAGAYWAAIVMMLGTGILASLIPDMGSQKRESKGAASGAPGMARLARSHIKVFATV
                     GVGILLVSGLRASRQIIIPLWADSLGLGPASTSIIFGLVAAIDMLAFYPAGKAMDQRG
                     RRSVAVPSTIIMGLSLILIPATNGLITFVIVSMLMGLGNGIGSGLVMTLGADASPSAG
                     RTQFLGIWRLISDIGASGAPVLLSIITAAASLAVASIAAGVMGFMAAGIFLHWLPSTG
                     RSRTP"
     gene            24850..25827
                     /locus_tag="VFR20_00195"
     CDS             24850..25827
                     /locus_tag="VFR20_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU0228965.1"
                     /translation="MAHVTVITTGGTIASTRASADGPVTANVSGNDLLASLHGTPIGI
                     DVTVEEFCNIGSNALDLPTVHALLARIRAALAKPDCTGVVVTHGTDTMEESAFLSDLL
                     VDSSKPVVFTGAQRHASEPDTDGPRNIRDALIVAAAPNARELGTLVLFEGDVHAARDV
                     TKTHASRVDTFRSPGLGKLGEVDQGVFHLYRRPAQRRTLADHGLDEHVELVKLGLGVK
                     PTYLEWCADNGVHGVVLEAFGRGNAPKGFAAAIARLVSARTPVIVASRCPEGQTALIY
                     GADGGAVTLAEAGAVFAGDLSGVKARLMLCVLLGAGATLPEIRAAFEPA"
     gene            26029..27282
                     /locus_tag="VFR20_00200"
     CDS             26029..27282
                     /locus_tag="VFR20_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0228966.1"
                     /translation="MSDMEHGQRAGITRRGVLKVGAAGLLTGMAGASPFIRSALAAEP
                     IKLGVLLPKSGTYAVQGDQGHNGSVIAVEDVKGEVLGRPVQLVWLDENGPQTTQQNMH
                     RLIQQEKVVSVSGGVSSGDILAIMPVAEREKILLMATGPNATEITGSKCNRYTFRVDL
                     PNYETVRSVYPTLEKHGKNWYFLYASYAWGIDGYNQMSLLLKQGGGKILGADQTPLGT
                     TDFSSYVLKIMAAKPDVIFLGLGGGDMTNFLKQFHQMGLTGKIPVSSLICNDTDMWAA
                     GPEAATGLYPKIWNYTGAQNTEASHAFAKKYQAKFGTPPESEAWQDWFGTTAILTAIK
                     ETGSTDTQKLIDFLESHKFEGYKKEAMYFRSWDHQLIQPTLVAEVKPKITTKYDYFNI
                     VAVAPGEGKPLESLYGSKSESECKM"
     gene            27394..28272
                     /locus_tag="VFR20_00205"
     CDS             27394..28272
                     /locus_tag="VFR20_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0228967.1"
                     /translation="MINATLFASQLMNGLVLGFLFVLIAVGMSIIFGMLGLVNFAHGA
                     FFALGAYFALSLRPYVGFGGVLVLAPILVGIIGLAIERTLLRPLYDKEPLLSLLLTFG
                     LALFIEELIRTLWGSMGHPFDTPAFLAGFFHYGPVFVTRYRLAVLVITVVILAAIWLF
                     LEKTPYGRIVRAGSRDPEMVSMLGINLKRVFMFIFALGCLLAGAAGVLAAPLWSVMPS
                     MAEQAIMPAFVVVVIGGLGSLRGAIVAGLMVGIAVAMTIQFWPVASTSIMYVLMIIVL
                     LLRPRGLFGEHWERFE"
     gene            28269..30773
                     /locus_tag="VFR20_00210"
     CDS             28269..30773
                     /locus_tag="VFR20_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEU0228968.1"
                     /translation="MNRPRSALTHPLVVLVAVFIIMPWLMTLAGSTISLATQVVIYTL
                     YALAYNLLLGYAGMVSFGSSVFFGMASYASALFAIHVYQNVALALIVGTAFAALLGLV
                     LGMLILRRRGLYFALLTLAFTQLFYEICFRWTSLTGGENGLQGVLRPGLENAWHYYVF
                     CAIIVAICAWLLYRIAHSPFGRVLQAIRDNEKRVQYLGYNPWGYKLSALVLHAAFIGL
                     GGSLLSLLIHGVYADNMNWQHAGDPVMMTLLGGIHHFLGPLWGAIIYINLSDQLSSFT
                     EHWWLFFGALIIIVVLLSPEGLSGVYARLRGHDRWGLTRTPTPAMPPSLPDPFAARGA
                     TYEGAILEVRHLSKRFGRVVTADDISLQVRAGTIHSLIGPNGAGKTTLFNMLTGLIPN
                     DSGQILFKGGDISKMAVHERTRLGMSRSFQIVSLPANLTVFETVRIAVQAGSPLRASL
                     WRNAYDLTDLVDRTWALLHAVGLEDKAGETTINLPHGEQRLLDIAVTMATGSELLLLD
                     EPLAGLADADRERIGTLIRQLAGKYTILLIEHDIDRVISLSDRITVLHQGHLIADGDP
                     HSVVNNPQVVEAYLGEAINDDGKSAPSTLAADGVTRDIGKTILQLRDVASGYGGSHVL
                     DGLSLEVREGEAVALLGRNGVGKTTTLHTIMGQIAVNDGHIRFNDTDITGWPSNRVNR
                     QGLAIVPQGRRIFPNLSVVDNLLIARRPGGWEPNQVYDLFPKLGTLRDAIGGNLSGGE
                     LQMLAIARALMAPTQLILLDEPFEGLAPAIVNEVLDAVIQLRQRTSILLVEQRVDLAL
                     RMVDRAYIMVNGRIAYEGAAAELKENKALQVKLLGV"
     gene            30951..31733
                     /locus_tag="VFR20_00215"
     CDS             30951..31733
                     /locus_tag="VFR20_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0228969.1"
                     /translation="MFDETSMKDRKVLITAGASGLGLEMAKAFTAAGATVLVCDVDQN
                     ALEAASAALPGLHTAVADVSDEASVAALFQQVDTQLGGLDVLVNNAGIAGPTGYVETL
                     SKIDWDRTLAVNITGQFLCARLAVPLLKQSKAGVMINLSSAAGHLGFAGRSAYSASKW
                     AVVGFTKTLAIELGKFGVRVNAILPGAVEGPRIRAVIAAKAQTLGVPVDEVAHSYESQ
                     ASLGHMVSARDIANMALFAASDAAASVNGQALVVDGHTQALV"
     gene            31735..32685
                     /locus_tag="VFR20_00220"
     CDS             31735..32685
                     /locus_tag="VFR20_00220"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEU0228970.1"
                     /translation="MPNDTMNIAIIGSGLIGQAWAIVFARAGCQVRLWDGDSAALNRA
                     VELIARQARELQKQRLLDDADALTTRVQACDDLGAALKGAAYAQENLPETLSIKKDIF
                     KRLDTLAAPDTVLASSTSSIPASAFTEDLPGRGRCLVAHPVNPPYLVPVVELCGAPWT
                     DAGAVETARAIMQTVGQKPVVVRRELEGFILNRLQGALLREAFRLVQNGYVSVEDLDA
                     TVKDGLGLRWSFMGPFETIDLNAPNGLADYCGRYGGMYESIAKEQTDTDAWPAGLINE
                     IEQQRRALLPGSGLLDRRQWRDQRLMALLRHKKEMETPDT"
     gene            32762..33694
                     /locus_tag="VFR20_00225"
     CDS             32762..33694
                     /locus_tag="VFR20_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEU0228971.1"
                     /translation="MAKKHKAIITCAVTGAIHTPSMSKYLPVTADEIADAGIAAAKAG
                     AAILHLHARDPKDGHPSQDPELFRPFLKKIKANTDAVINITTGGSPHMTVEERMRPAM
                     TFKPEVASLNMGSMNFGLFPMLDRFKEFKHDWERQHLENSRDLIFKNTYKDIETILRK
                     GTENGTRFEFECYDISHLYNLKHFLDHGLVQSPPFIQSVFGILGGIGPDPEDLMHMRR
                     TANHLFGDNYEWSILGAGRNQIPLATMGAAMGSNVRVGLEDSLWIGPGKMAASNAEQV
                     SRIRVILEALNIEVATPDEARETLHLKGGDKVDF"
     gene            33743..34498
                     /locus_tag="VFR20_00230"
     CDS             33743..34498
                     /locus_tag="VFR20_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0228972.1"
                     /translation="MFDLTGQVALVTGSSRGIGYAAAQALGRQGATVALNGRDPDTLE
                     NAANDLRSQGITVHTAPFDIADVAQATHAVDALQKATGKIDIFFANAGIQHREPLLSF
                     PQVEFERIVFANLTAQWALGRYIAAGMSERGYGRILFTGSITALLGRKEITAYTAAKA
                     AIHGLVRQWATELSVSGVTVNAIAPGYIRTELTRNLWADEEFNHWLEGRVPQKKWGNP
                     EDIATAVVFLAARESAFVTGQVLVVDGGMSSVM"
     gene            complement(34581..34657)
                     /locus_tag="VFR20_00235"
     tRNA            complement(34581..34657)
                     /locus_tag="VFR20_00235"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34621..34623),aa:Arg,seq:tct)
BASE COUNT         6719 a        11569 c        10347 g         6106 t
ORIGIN      
        1 ggctacgacg aggaactcga caccctgcgc cagctggcgg gcgacaacgg cgccttcctg
       61 atggagatgg aaacccgcga gcgcgaacgc acgggcatcg ccaatctgcg ggtggagtac
      121 aaccgggtgc acggcttctt catcgaggtc acgcgcggcc aggccgacaa ggtcccggac
      181 gactaccgcc gccgccagac gctgaagaac gtcgagcgct acatcacgcc cgaactgaaa
      241 tcctgggaag acaaggtcct gtcggcccgc gaacgcggcc tggcgcgcga aaagctgctg
      301 ttcgaccaga tgctggcaca ggcggccggg catgcggcgg ccctggcggc cagcgcggcg
      361 gccctggccc gcatcgacgc gctggcggcg ctggcgcacc atgcccgccg ccatgcctgg
      421 gtcgcccccg aactgtgcga cgacaactgc ctggacatcc aggcgggccg ccacccggtg
      481 gtggaacaca gcatcgagac cttcacgccc aacgactgcg tcatgcacga agaacgccgc
      541 atgctgctga tcaccggacc caacatgggc ggcaagtcca cctacatgcg ccaggtggcg
      601 ctgatcgcgc tgctggcccg catgggcagc ttcgtgccgg ccacgcgggc gcgcatcggc
      661 cggctggatc gcatcttcac gcggatcggg gcggctgacg atctggcggg cgggcgctcc
      721 accttcatga tggagatgac cgaggcagcg gcgatcctgt cggcggccac cgcgcagagc
      781 ctggtgttga tggacgagat cgggcgtggc acatccacgt atgacggcct ggccctggcc
      841 tggtccatcg cccaccggct gctgacgcac aaccgcgccc tgaccctctt cgccacccat
      901 tacttcgaaa tcacgcgcct gcccgccggc acgcaaggcg ccgtcaatgt ccatgtggcg
      961 gccaccgagt cagcctcggg catcgtcttt ctgcacgagg tccgtcccgg ccccgccagc
     1021 cgcagctacg gtatccaggt ggcgcaaaga gccggtatac cgcccgccgt catccgcctg
     1081 gccagccggg agctggcgcg attggaggcc cgtggcgccc ccacaccgca actggatctg
     1141 ttctcggcgt cgacgcctac ggcggatgag gccgacgacg gcgccgaaaa tgatatgacg
     1201 acacgcatcg aagcggccga tttccacgag atcgccggcg acgcatccga cgaacgcgcc
     1261 ctggctctcc aggccgacct cgccgacatc aacccggaca gcctgagccc gcgcgaggcg
     1321 ctggacatcc tctaccgcct gcgcgccgac tacctatgag cccgcgccat ctatgaaccc
     1381 gtaccaccca tgaacccgcg cacccctctt ccgattcttg gcggcctcag cccgcaggcc
     1441 ttcatgcgcg accactggca acgcaaaccc ctgctcatcc gtcaggcgat tccgggcttc
     1501 aggccgccgc tgaccattcc agagatccgg caactggctc gccgcgacga ggtgcaatcg
     1561 cgcctcatct ggcgggatga ggcgggttgg cacatgaagc acggcccgtt ctcccgcctg
     1621 cccgccccgc accgtcctgg ctggacactg ctggcccaga gcgtcgacca gcatgacgac
     1681 gaaaccgcgc gcctgctgca ccaattccgt ttcatcgccg acgcccgcct ggacgacgcc
     1741 atgatcagca tcgccacgga cggcgggggc gtgggcccgc atttcgacag ctacgacgtt
     1801 ttccttttgc aggctcacgg acgacgccgc tggcgcattg gcgcccagaa ggacctgtca
     1861 ctgcgaccag ggctgccgct gaaaatcctc caggacttcc gtccggacca ggagtatgtc
     1921 ctggaaccgg gcgatatgct gtacctgccc ccgcacctgg cgcacgatgg cgtggccctg
     1981 ggcgagtgca tgacgatttc catcggtttc cgtgcgccga cgctggcggt gcttgcgcgc
     2041 gggctgctgg acgccgccgg tgaccaggtc accgcgcgcg cgggtggtga caccggcctg
     2101 tacgccgatc ctccgttgcc cggccccgcc ctggcgcggg tcttcgcgga tgccggcgag
     2161 ccggccaccc ccacgcccgc ccgcctgcct gacagcttga tacaagcctc gctgaacgcc
     2221 gtggcgcaga tgcgtttcga cgaaaaactc gccgcgcgct tcctgggcca atggctgacc
     2281 gaaccgtcgc ccgcggcggt gttcgaacct gaaccgaagc ctctccatct gtcgcacggc
     2341 ctgccggcca cggggcgcct ctgcctggat cgctgcacac gcctgatgta ccggagccgg
     2401 gaactgtaca tcaacggcga agtcgccgac ctccccgcat ccaaggcgtt gaggacgctg
     2461 gcggaccggc gagaacttgc ttgcgccagc acggccgcac ggtcgttgac cgcgcctgaa
     2521 cgatccatgc tcgaaggatg gctggaggaa ggttggctgc attgccacga ctgactgtcc
     2581 tcaaggactc ttcgtcgaag ttgagcggaa agccggcgcg gtggctcgtt cacaggagcc
     2641 ttaataaggt caacgctcat aaacatatat tcaaataaca aaataagaat cttgtcgttc
     2701 gcgagagtgg cgcggtttcc tagaatgacc gctgatccca cttttccgag cgaaccatgc
     2761 tggccccgct atccctccct gaatccaagc agaccctgct cggcgaattg actcaaggcc
     2821 tcgagcccga tgcgctcaac tggctatcgg gctacttcgc gggcatcgcc gccgcggcgc
     2881 gccctgaacg cggcgtcgcg cccgtggtgg ccgcggtgcc gtccgccccc gcgatgcgcc
     2941 cactgacgat cctgtacggc agccagaccg gcaacgcgca gcgcaccgcc aaggcgctcg
     3001 ccgagcatct ggaaaagctg ggcctgccga cccgcctggt gcgcgccgac cgttacgcga
     3061 cgaaggagct gaaggacgag caactgttgt acgtcgtcat cagcacacaa ggcgatggcg
     3121 atccgccaga tgattcattg gcttttgtcg aattcctgaa cagccggcgc gcgcccaggc
     3181 ttcccggact caagtacgcc gtgctcggcc tgggcgactc cagctatccg gtcttttgcg
     3241 gcacagcctt ggccctcgac gcgcggcttg ccgagctggg tgcgcaacgt ctcttcgacg
     3301 cgggcacggc cgacctggac gtggaaaccg tcgccacgcc ctggcgcgac aaggcgctgg
     3361 agcaggcgca aaccgttctg ggacagtccg aggcaccccg cgccagcatc acgccgctgc
     3421 ggcccaaggc cactcgcata tcccgtgaca acccattcca ggccgaactg ctggccagtc
     3481 aacccatcac ggcgcctggc agcatcaagg atgtgttgca tctggagatt tccctcagcg
     3541 gcagccagtt gagctaccag cccggcgacg ccttgggcgt gtggccgaca caggcagaga
     3601 cgctggtcga caacgtcgtc tcgaccctgg gcctggacgc gaacgaggcc gtggaagtcg
     3661 gcggggtctc acgcccactc cgggaatggc tgagccatca tagggaactc acgcgtctaa
     3721 cccgcccgtt cctgatcgcc cacgcgaaac tgtccggtag cgacgaactg ggccggctac
     3781 tggatgacga cgcgcgcgac gccttgcggc acaccctcga aacccggcaa ttgatcgaca
     3841 ttctcaggca gtatcccgcg ccctggaccg cgcaaggcgt ggtccaggcg ctgcgccccc
     3901 tggccccccg catgtattcc atagcatcca gccaggccag cgtcgaagat gaagtacacc
     3961 tcactctcgc caacgtcgcc tacgaccacg agggcgaagc gcgctggggg gtggcctcga
     4021 actacctgag ccaactggac gaaggcgcac gactgcctat cttcatcgag gaaaacacac
     4081 gtttccgcct gccgaccgat ccggcgcgcg acatcatcat gatcggcccc ggaacaggcg
     4141 tggcgccctt ccgcgcattt gtccaggacc gcgcggcgcg cgccgccccg ggacgcaact
     4201 ggctgttctt tggcaacccg tacttccaca ccgacttcct ctatcagacc gaatggcagg
     4261 gtgcgctggc cgatggcagc ctgcaccgcc tggatctggc gttctcgcgc gaccaggaag
     4321 aaaagatcta cgtccaggac aggctgctgg aacacggcgc cgacatctac caatggatac
     4381 agggtggcgc ccatgtgtat gtgtgcggcg acgccacccg catggccgtc gacgtacagc
     4441 agaccctgct gaggatcgcg tcccgggaag gcggcctgga cgaatccggt gcgcggcaat
     4501 ggctggacga tctggccgcc cagggacgct acgcccgcga cgtgtactga cctctgaccg
     4561 gcaacactga cacgcagaac cccgcgcgcg gccatttccc cgcgcgcatt caggatcacc
     4621 atgagcgaca ctcccctttc ccatctggaa catgtcaagc aggacagccg ctacctgcgc
     4681 ggcacgatcg agcagggcct gaacgacgcc gtcaccggcg ccatcagcga cgacgacaac
     4741 aaactcctga aattccatgg cagctaccag caggacgatc gtgacatccg cgacgaccgg
     4801 cgccgcagaa agctggagcc cgcctatgcg ttcatggtgc gggcgcgcct gccgggtggc
     4861 atcctcaccc cgtcgcagtg gctggcgttc gaccatatcg ccacgacctg ggcgacgcgc
     4921 gggctgcgca tcaccacacg ccagacattt caactgcacg gcatcctgaa acgccacctc
     4981 aagcccgtga tgcaggcgat ccacgacgcc atggccacca ccatcgccgc gtgcggcgac
     5041 gtcaaccgcc aggtggtcag ctcggtgaac cccctgctct cggaccagca ccagatcgtc
     5101 caggactgga ccgaaaagct gtccgcgcac ttcctgcccc gcacacgggc ctacagcgag
     5161 atctggctgg acggcaagcg cgtggacacc gagccgagcg acccgttcga ggtggaaccc
     5221 atctatgggg ccgcctacct gccccgcaag ttcaagatcg gtatcgccat tccacccacc
     5281 aacgatgtgg atgtgttctc ccacgatctg ggcctgatcg ccatcatcga gaacggtgtg
     5341 ctgcgggggt tcaacgtcgc cgtgggcggc ggcatggggg ccacccatgg tgatgccaca
     5401 acctaccccc ggctgggcag cctcattggt ttcgtatcgc ccgacaaagt catctcgctg
     5461 gccgagggcg tggtcaccgt gcaacgagat ttcggcaacc ggtccgagcg ccagcatgcg
     5521 cggttgaagt acaccgtcga tcgcatgggt ctggacgcct tcaccgccga actggaacaa
     5581 cggctgggct acgcgcttga acccatgcgc ccttaccgct tcgacagcaa cggtgaccgc
     5641 ttcggctgga tcaagggtga gaatgacacc tggcacctgg gcctgtacgt ggagtcgggc
     5701 aggctctggg acacggattc actgaaactg ctgaccggca tgcgcgagat cgcgcgcgtg
     5761 cacaagggct tgttccgcct gacgtgcaac cagaatctga tcatcgccgg tgtcgccgac
     5821 gcggacaagg ccggcatcga tgcgctcgtg cgcgaatatg gactcgacga ctacacacga
     5881 agccccgagg tccaccgtca ggccatcgcc tgtgtggcgc tgccgacttg cgggttggcc
     5941 atggcggaaa gcgagcgcta tgcgccggtg ctgctcggca aggtgcatgg cctgctggac
     6001 aagcacagcc tgggcgacgc gccgattcta ctgcgcatct ccggctgccc gaacggctgt
     6061 tcccgcccgt atttggggga gatcgcgctg gtcgggcgcg ccataggccg ctacgacctg
     6121 agactcggtg cgaacttcac tggcgagcgc ctgaacgtcc cgtaccgcga gaacatcacc
     6181 gaacccgaaa tcctggacgc cctcgacgcc ttgttcgcct cgtatgccga ccagcgtcat
     6241 gacgctgaga agttcggcga cttcctggtt cgctccggcg tgattccggc acctgcccgg
     6301 aacatcatcc ccatcattca caagcccctc cgcacatgaa cctgtttccg ctattcctca
     6361 gcctcaccgg caagcgggtc ctggtcgtgg ggggtggccc gatcgccgaa cgcaaaacgc
     6421 gcctgctgct cgacgcgggc gcccgggtga cggtcggtac gctgacggct tgcgaggaac
     6481 tgacgagcct gtcggcgcaa ggccatgtca atctcgtgat cggggcgttc gaggaacact
     6541 ggctggatcc ggtgtggttc gtggtggtgg cgaccggaga ccatgcgctg aaccaagaga
     6601 tagcacggcg gtgtgaacag cgacggctgt tcgtcaatgt cgtcgacgat gccgaacatt
     6661 cctctgctca ggttccggcg attgtcgacc ggtctcccgt gaccatcgcc atttcctcgg
     6721 gcggccatgc ccccatgctc gcgcgtcgtg tgcgcgaacg gctggagaca ttgctcgatg
     6781 gctccctggg accactggcc acgttggccg gtaagtatcg ccgcgcgata caacggcact
     6841 ttcccgacat gcgcaagcgg cgcggattct atgactggct ggccgagggg ccggttcatc
     6901 aagcgctggc ggccaacctc cccgacgcag cggaacacgc cctggagcat ggcctgatgc
     6961 atcactccga caccagcgaa ggcatggtgg cgctggtcgg cgcgggtccg ggagatgcgg
     7021 gcctgttgac gctcaacgcc ctgcgcgcac tgaaccgggc cgacgtgatt ctgtatgacc
     7081 gcctcgtcag ccccgacgtc ttggcgctcg cccgccgcga cgcgacgcgc atcgaagtgg
     7141 gaaagcgtcc cggcgaagac cacgacgcca cccagcgcgg catacacgag ttgttgctgc
     7201 gacatgcaag actaggccac tacgtcgtac gactcaaagg gggcgactcc ctggtgttcg
     7261 gacgcggtgg cgaggaactg gaatacctac gccagcatcg agtgcgatat caggtcgtgc
     7321 ccggtgtcac tgcggcgctg gcgtgcgcgg cctattccgg agtccccctc acgcaccgcg
     7381 atcatgcgca atcggttcgc ctcgtgaccg cacatggccg cgatggacat gacgaacccg
     7441 attggcgcga cctggcgcga cctggccaga ctttggcgtt ctatatgggc gtcagccgcc
     7501 tacccacact ggcggccaat ttgatggccc acggccgaag cgccgacacc ccgttcgcat
     7561 tggtggaaaa tggcacccgc gcggatcagc gtgtgctgac aggcaccctc ggtcgcctgg
     7621 ccgagttagc cgcacgacac caattcaagg cgccggcgtt gctattgctg ggcgaagtcg
     7681 ccgcactggc cgacaccctg cactggtacg gcacccgcgt tgaaaccgac gcgcccgggc
     7741 atgcgggcat ccacgattcc cgcacggatg cgatcatcgg agagtccctg ggcgaaaccc
     7801 tactgccagc gtagaataaa tgacgtcgat actcgccgcg caagcggacg ccggggcaga
     7861 tggcaaccac cgactttgac gatgatgacg ttttctcgga caaaccagag cgcttccccc
     7921 gcttcgggtg ggaggccctg ggcagcatgt cgccggccta cttcggcatg gtcatggcga
     7981 ccggcgtggt gtccatcgcc gccaatctgt ccggccacga caccatcgcg cgcgcactgt
     8041 tcatcctgaa catcgttttc tatgtcgtgt tgctggtcct gtcgggcctg cgcacggtgt
     8101 tcgcattgcg ccgcgttctg ttcgacctca cccaccatcg ccgcgcgccc agcttcttca
     8161 cttccgtggc gggcaccagc atcgtgggga tccagtgcat agaattcgcg caccggcccg
     8221 acatcgccat cggcctttgg atattggcca tcatcatgtg gctgggcctg acgtattcga
     8281 tcttcaccgc gctcatcatc aagaccaaca agcccacact ggatcacggc atcaacggca
     8341 gttggctgct ggcggtagtg gccacgcaat ccatcgccgt gctgggttcc atcctgaccg
     8401 ccaaccacgc gctgcctgac cggttggaaa tgaatttctt cgccctgtcc atctggcttt
     8461 ggggcggcat gctgtacatc tggatgatct cactgatctt ctatcgcgat gtcttcttcc
     8521 gcttctcgcc ccacgatctg gcccctccct actggatcaa catgggggcc atggccatct
     8581 ccacgctggc cggatcactc ctcatactca acgcgggcga tgcgccctac ctcaaatcgc
     8641 tgcttccatt cttgaagggg ttcaccattt tctactgggc caccggtgtc tggtggatcc
     8701 ccatgctcat cgtgctggag ctttggtgct acgtgttcaa gaaattcccg gtgcgctacg
     8761 atccactgta ttggggcgcg gtgtttccgc ttggcatgta cgccgcaggc acctacacca
     8821 tggacaaggc catgcatttc ggttttctcg aaccgctgcc caccgcgttc ttctatatcg
     8881 cgctggttgc gtgggccgcg acattcgtgg gtctggcccg gcagttgtgg gaccgcgcca
     8941 tgcatacctg gcggccgggg gccgcgcgtg gctgaagcgc ctgttttcca aacaatccct
     9001 cggcaccgca aggcgaaaca gaccacggaa cgcgaaaaag ccgggtcgga ggaatccccg
     9061 gcccggcctt tttcgatacg aaccctgtag cgggtcaaga acccgacggt gtccccatat
     9121 gctgcgacag cacggtgatg atgcgcttgg ccgtggcgct atcatcgcgc ttgccgttgg
     9181 cgtcgagcac cgtgaccagg gaggatgtcc cgtcactttg ttgggccaca tgcaaccgat
     9241 atttcgtcgg cgccgcggtg ttcttcgtgc cgaacagtcg accaatgaag gtctgctgtt
     9301 cgatcttcac accggtgtcg gtatccaggt agctgatgta gtaatcaccc gctgagcgat
     9361 cgcgatcatc gaccgagaaa ccggccgaat ccagtgcgat gccgacacgc cgccaggccc
     9421 gatcgaacgg ttccgacact gtcaggtcgg cgctctggcc agcggtctgg gcctgaacct
     9481 tggtctgggt ggggtctttc accgcctggg cgatgagcgt atgggcttcg ttgacattgg
     9541 tacccaggta caccatgaga cgcgccagca tggcggcgtt caacccctgg tcctccttgc
     9601 cgggcaccca cttggacgtg gaaaagtcgg gcgagacttt ttcaaccatg tgctggtgac
     9661 tgatgtagat ttcagtgtgc ccgttcaccc gttcgatgcg ggtacggaag cgatcgcgtt
     9721 cgccgctgtc gaacacctgg tcgagcaacc cgcccagagc agagcgtatc cagccttcgg
     9781 gaatcttggc gcggttctcg gcccagtccg tctggatgag acccgccttg ggatcctggt
     9841 tgtggatggt gaagccttgg tcgccccaga actcgaccac cttgggatag atgctctggg
     9901 ccggctggtc gaccaccagc cagtggatgt cgccgtcgcg catcactttc atgcccggtg
     9961 tgttcggcag aacggcgttg tcggcgtttt gcgcattgcg ggcggcctgg ctttgcgcgt
    10021 acttgctgta cgtggccata ccttcgggcg cgcggtagtg cggattgcta tcggcctggg
    10081 tgaggtcggg cggaatggtg aggggctcgc ccgccaccgt gctcttgtag ttcacggatt
    10141 cctgatcgct catgaacttg ttcagcgaac accccgacag ggcaagcagg ctcaatccca
    10201 ccaccacggc ggtggcgtgt ttcttcaggc gcataatcgt cattttcaga gtagtcccgc
    10261 gtctttcatg gcatcgcgca cgaccgcgcg atactgttcg tgcaaagggg tcaagggcag
    10321 cctgtacccc agttcagtca gcccgagttc agcaacagcc cacttcaccg gaatagggtt
    10381 ggactcgaca aacagggctt gattcaacaa cgccagccgg ccattgagct ctcgcacacg
    10441 gacggcgtcg cccgccagcg ccgccgcgca caattcgtgc atgagcttcg gcgccacgtt
    10501 ggctgtgacg gaaatgttgc cgcatccgcc caggagaatg agcgcggccg cggtgggatc
    10561 gtcgccgctg ataagcatga attccttggg gacacggcgc agcagatcgg cgacccgcgc
    10621 gatattgccg gtggcatcct tgacaccgac gatacccggc acccgcgcca gccgggcgag
    10681 ggtctcgttc gacatatcgg cactcgtgcg gcccgggacg ttgtacagca cggtgggcag
    10741 atccacggct tcggcgaccg tcttgaaatg ctgatacatg ccttcctggg tcggtttgtt
    10801 gtaatacggc accacggaca gacccgcatc ggcccccacc gccttggcat ggcgcgacag
    10861 gtgaatggct tcggaggtgg agttcccacc caccccggca atgaccggca gccgcccggc
    10921 ggagtgttca accgcgatcc ggatgagttc ggcttgttca tcgacgctga cggtgggaga
    10981 ctcgccggac gtgccgacca ccgccagcac gtcggtaccc tgctcgatgt gccagtcgat
    11041 cagttttcgg taagcgttga aatccaggct gccgtccggg tgcatggggg tgaccagggc
    11101 gaccatactg ccctgaaaac ggggaatcgg ggaatccgtg ctggccatga tggaaatgat
    11161 ctcctgaaac acgcggtctc gttgcgcccg gctggctctt gccaggcatg cccgccaagc
    11221 ggccgcccga tgcgtcgggt gacccggttt ggcggtcaac gtattagttt aacggattgg
    11281 cgcgcgaatt tataaaaacc acctgaccac cgcctggcca aaagccagga agggggtcag
    11341 cacgaaccac aagcccccgg caagcatcag cacgatcagg atcaccaggc catagggctc
    11401 gatcttcgaa tagcgccagg ccatgcgcga cggcaatagg ctgaacacga tgcggccacc
    11461 atcaagcggc ggcaggggaa tcagattgag cgccatcagg atcaggttga cctgaatgcc
    11521 ggcgatcgcc atctgtaccc agaaatcggc ctgaccggcc cctgtggacg cgagcatacg
    11581 cagcgacagc gcccacagca ccgccatcaa cagattggag gccggaccgg caagcgccac
    11641 ccacagcatg tcgcgcttgg gctggcgcag ccggctccag tcgacgggca ccggcttggc
    11701 ccaaccgaag aggatgcccc ccatgcccag cagcttgctg ccgaacagca agaccagggg
    11761 gaccacgatc gtgcccatgg ggtcgatgtg ccgcatgggg ttgatgctga cccgcccagc
    11821 ctgatacgcg gtcgggtcac cgaataagcg cgccacataa ccgtgcgcgg cctcgtgcaa
    11881 tgtaatgccg aacagtaccg gcaaggcgta tatggtgatg gtttgaatca gggaatccat
    11941 gggtttccag aagaaggacc gggcaccggt cggcaacctt aacacactgc gccaggcacg
    12001 gccgcaacgt catgccaagg tattgcgccc cgcaggcgcg agccccttcc attttccctc
    12061 tattttcctg tcagcgtggc gctccctctt cctgtggggc ggaccctctg cggtgcgaac
    12121 aggtcttatg cctcgatgcc gagtccggcc agattgccct gcccgcgccg gatgacctgc
    12181 ggcacaggcc cggtcagatc gaggaccgtg gtgggcatga gcgggcaggc gccaccgtca
    12241 atgacagccg ccaattgatt accgtagcgc tggtagatgt cctcggcgtc gttcaggggc
    12301 tcatcctcgt cttcgggaat cagcgtggtg gacatcagtg gcgaccccgc atgctcgatg
    12361 agctcgagcg cgatgcggtg atcgggcacc cgtatgccga tggtcttgcg cgacggatgc
    12421 gacacacggc gcggcacttc tttcgtggcc tcgaggatga acgtccaggg gccgggcgtg
    12481 gcctttttca ggagccggta ttgggcatta tcgacccgtg cgaaatgacc gatctcagcc
    12541 agatcgcgac acaacagcgt caggtgatgg cgttcgtcca acccccgcaa ccggcgcaat
    12601 gcgtcggccg ccgccttatc gtcgagacgc gccaacaccg catagctgga atcggtgggc
    12661 accgccacca aaccaccgcc cgcgagcaac tgccccgcct gcctgagcag acgggcctgc
    12721 ggattctgag aatgaacgac gaaaagctgt gccattgaat aatcctagaa tgcgatgcct
    12781 tccaggcacc gggcgcgctc gcacccaatg cgaaaccagc ggcgaaccca cgaccgggtc
    12841 aatgatagcc gctgacggcc gccacacaca ccacaatcag cccgagcacc agattgatcg
    12901 cgatcagcat acggatctga ttcgctttac cggcggcaag ttcccagtcg ttggccgcga
    12961 ccgcctgttt caggcgacga aacggtgcga aatacacatg caggaagatc aggatcatga
    13021 tgccgccgag ccccatcatc acgtggataa gcccacctgc gttggccatg cccccgaatt
    13081 tgccgaacac cgcccacacc cccgtgacca gcaacaccac gaccgccagc cagacccatg
    13141 cgaaaaaccg cgcgcaaacc accgcccaca gccgaagctt ctgttgcggt tcgagcaccg
    13201 cgtccgccgc cggacgcaat gccagatagg cgaagaacat gccccccacc catatgacgg
    13261 ccgaaagcat gtgcagaatc acgatgagtg ccatacgtca acctccttga ctcgcgctga
    13321 acaacgcgaa acatccgaac cgcccaccgc cgtgacccgg acgccgggca gcgccaatgt
    13381 ctatagccta acaccagcct tcacgcccca gtgccctgat ttggggggtg attgaaaaat
    13441 aaatcgaccg gaatcttctg gaggccgata tccatgcctc cgaaaaaaca agcggcggat
    13501 ccccatgcca agcatggagg gtccgccgac aggccgcccg tcttgacgcc tagcgcctcg
    13561 gcaggccagc acacttagag tgcccgcatc tatttcttgt gggacgaatt cattttgtcc
    13621 atgctcgacg acgacgagga gttcgactcg ttgacggaac tggcgggaat cgtggtttcc
    13681 ttgatggtca gggtcggctg gacgtcgatc ttctgggcct tgatccaggc ttcatgctga
    13741 gcctgggttt cgtgggcctg aacggtggcg aacggcgcag ccagcaatgc ggaaaccaga
    13801 agggtcttat agagctgttt catatcatga gcaccttttc aagaagattg ccgaactatt
    13861 atcgtagagg aacggcaact ctttgcaatg atacaaacgc ttcaatttgt cacaatatca
    13921 gtgcgacaac ttcagccgtt tcatcagggt tttcacggat ttcgggggta catagccctc
    13981 ctgcacgttg tttcaatcag aaacatctgg ccgcgcggaa gccccggggt tcgcacaatg
    14041 gcgacacact cggaaagtgg ccagaaccag ggggcgggaa gagttggggt gaccgatggg
    14101 gctcgaaccc acgacaaccg gaatcacaat ccgggactct accaactgag ctacggtcac
    14161 cactgacacc ggctcgcgca acgacagaag aggttggctc accagcaaga tccgggcaga
    14221 acgccagaat caaagaaacg ggattctagc atgaagtcca gacgaaggca tcacacaaac
    14281 cccctccagc atcgaatgaa tctggaaggg cgccgcgacg cccgcatcgg atgatgcctg
    14341 gccgcatagc gccacgcctg gcctgcattt gacgtatgat tgattcactc attcatcaca
    14401 tcaatcgttc atgcgcgccg gctgccaatg gaacaacaca ccggccgcac ggtggtcttc
    14461 cattatttcc tccattcgac cgaatccgtt cccccacccc gtcatgtcca acatcaaagc
    14521 cgttgaattc gaacgcccct cccccgttcc cggtgcccgc gtcaccgcgg aggcatgggc
    14581 tcgcgtgcca gcacctgtgt cgtccgacca gaaatccgcc ctgaaaaacc gcatcaagga
    14641 actgctgcgg gcacagcggg ccgtgctggt ggctcattac tacgtcgatg ccgacatcca
    14701 ggacctggcc gaggaaaccg gcggttgcgt gtccgactcg ctggagatgg cgcgcttcgg
    14761 gcgagacaat cccgcacaaa cgctggtggt ggcgggtgtg cgcttcatgg gcgagaccgc
    14821 caagattctc agtccgcaca agcgcatcct gatgcctgat ctcgacgcga cctgttcact
    14881 ggacctgggc tgcgacccag tggaattcgg cgccttctgc gatgccaacc ccgaccgcac
    14941 cgtggtggtc tacgccaata ccagcgcggc cgtgaaggca cgcgccgact ggatggtgac
    15001 atcatccatc ggactggata ttgtcgccca cctgcatgaa cggggggaca aaatactttg
    15061 ggcacccgat cgccacctgg ggcactacat ccagaaacag accggtgccg acatgttgtt
    15121 gtggcaaggg tcgtgtctgg ttcacaatga attcaagggc cttgaactcg aagccctgcg
    15181 ccgcgaatat ccgggcgcca aggtgctggt acaccccgaa tcgcccgacg ccgtcatcgc
    15241 ccaggccgat gccacaggct cgacatcaca actcatcgct gcggcccaat ccatggacgc
    15301 cgacgtcttc atcgtcgcga cggaccaggg cctgttgcac aagatgcggc aagcagcgcc
    15361 cggaaaactg ttcatcgcag ccccaaccgc cggcaacagc gccacttgca aaagctgcgc
    15421 ccactgccca tggatggcca tgaattccct gcaaggcctg gcccacgtgc tggaaaccgg
    15481 cgtcaacgaa atcaccctcg acccggagat tgggcgccgc gcgctgggct gcatcgaccg
    15541 catgctggcg ttcgccgcgc aacgcaaggc aaacgtgcgc ccgggccccg acctggcgca
    15601 agaacaagaa ctcttcgctg gcgtgggtcc cgcatgaacg caaccaacac atcgccccag
    15661 ggcttcatgc cgttcgataa ctccctgcgc gcggcattcg aagccgccgt cgaatccgcc
    15721 ttgtccgaag atgtgggaag cggcgacgtc acagggctgc tcgttcctgc caccgccacc
    15781 gccaccgccg agctcaaggt gcgcgaggaa gccgtcctgt gcggcgcccc gtggttcgag
    15841 gccgtcatgc gccgctgcgc gccacaggcc caagtcgaat ggcgctacgc cgagggcgac
    15901 ctcatggccg ccaacagtct ggtgtgccgg gtgcgcgcac ccgcacgcgc gctgctcacc
    15961 gccgaacgca cggcactgaa ttttctgcaa ttgctgtcgg cgaccgccac cgcgacacgt
    16021 cactatgccg caattgtcga aggcaccaac gcgcaaatcc tcgatacacg caaaacgatc
    16081 ccgggcctgc gtctcgcgca aaagtacgcg gtacgcgtcg ggggcggcgc gaaccagcgg
    16141 ctggcgcttt atgacggcgt gctcatcaaa gagaaccata tcgcggcggc tggcagcatc
    16201 aaagccgtga tggaggcggc ggcgagcctg ccccgaacag tgccggttca aatcgaggtc
    16261 gagagcctcg accagcttcg ccaggcactg gaagcgggcg cgacatccgt cctgctcgat
    16321 aatttcaccc tggacgccat gcgggaagcc gtcgtcctca cggcgggccg agcgctcctg
    16381 gaggcatcgg gcggcatcaa ccacgacacc gtgcgcgcca tcgcggaaac cggcgtggac
    16441 cgtatctcga tcggcagcct gaccaaggac attcgcgcgg tggactattc cctgcgggtc
    16501 acgcaaatgg ggtaggcaat caaccgaccc gcaagcccgg ggtcaacacc gtgggcagtg
    16561 ccctgggcag ggtgcgcggg tagtcctggc aataatgcag gccacggctt tccttgcgtg
    16621 actgcgcgct ttccaccacc agggcggcca cctggatcag gttgcggcat tccagcatgt
    16681 tgccgctcac acggtaggca cggtagtact cggcgatctc ttcgagcaac aagtcgacgc
    16741 gatgccgggc acgcgcgaga cgcttgccgg tgcgtacgat gccgacgtag ttccacatca
    16801 ggcgccgcac ctcgtcccag gcgtgcgaga tcaccacggc ctcgtcttca tccacgacac
    16861 gactgtcatc ccagtcgggc acaccacgat cgggcggcaa cggcccggct tcctgggatt
    16921 caatgaaacg cgccacgttg cggcccacca ccaggcactc cagcaacgaa ttgctggcga
    16981 ggcggttggc gccgtgcagg cccgtgcaag cggtttcacc cacggcgtac agattgggga
    17041 tgtcggtgcg cccctgcagg tcagtgacga taccgccaca ggtgaaatgc atggccggca
    17101 ccacgggaat gggctgccgg gtgatgtcta tgcccagggc ctggcagcgc gcgcgtatcg
    17161 tcgggaaatg ctcttcgatg aattcgggcg ggcggtgcgt gatgtccagc tcaacgtaat
    17221 tcaagccgcg cttcttgatt tcgaagtcga tggcacgggc gacgatgtcg cgcggcgcca
    17281 gctccagacg ctcgtcgtgg cggctcatga atcgcaggcc cgcatcgacc tcggccccac
    17341 caggaatgcg cagaatgccg ccctcgcctc gcaccgcctc ggagatcagg aacgatttgg
    17401 cggacgggtg gtacagacag gtgggatgga attgcatgaa ctccatgttg ctgacccgac
    17461 accccgcgcg ccaggccatg gcaatgccat cacccgtgga ggtatcgggg ttgctggtgt
    17521 gcagatagac ctttcccgct ccgccggtgg ccagcacggt atgcgcggcg agaatggtct
    17581 cgacccggtc gttggcctcg tccagcacat agagccccag gcaccgggcc aggtcgcccg
    17641 cgttatcatc cgcccccggc acaccggccg gacgcccgcg cttgccgcgg gtaatgagct
    17701 caacggcgaa atgattttcc agcaaggtga tgttgggatg ggcccgcacc ttttgttcca
    17761 gcgtgacctg cacatgatgg ccggtggcgt ccgccgcatg gatgatgcgg cgctggctgt
    17821 gccccccctc gcgcgtcagg tggaacccca gctccgctgt gtcatcgggc gtgaacggca
    17881 ccccctggtc gatcagccaa cggatcgccg ccttgccgtt ctcgacaatc tggcgggtca
    17941 cgacctcgtc gcacaacccg gcgccggcgg tcagggtatc gttgatgtgc tgatcatggc
    18001 tgtcgtggga atcaagcaca gcggcgatcc caccttgcgc ccagtcactg gccccatcga
    18061 gcagggttcg cttggcgatc acgacgactt tcttgtcccg cgccaggtgc agtgcaacgg
    18121 tcaggcccgc gaggccactg ccgacaatgg ccacgtcaaa tcggttcatc tgagtattcg
    18181 tttcatcagg taaaggcgat ccccggcggc ctatggcgac acagccgggc gtccgcggga
    18241 agttgtcccg ccccggatat tctacgcgac tcgtcggcca actcatgccg cattcatcgg
    18301 caaccgggta tcattgcccg ttatgcaccc gcatccggtg tgtccggcgc ggtcctcggg
    18361 atcctctgaa caagtcgagc gggaagcagg cgctgtggat tggggttgga tgcaaggcgc
    18421 gaaccgcagc gatagccggt gctatcgcga ggattcgcaa cgcggcaaca gcctcaaccc
    18481 acagatgcat gcggcgcggc gacttgttca gaggatcccc agctcaaacc gacaaccccc
    18541 atgctcatgg tactgaccca ctggccccac attgttttcg ccttgagcgt ggtggcgggc
    18601 gccgcggcgg ccatacacgc cgccatgacc aaaaacgacg tgcggtcggc cattggctgg
    18661 gtcggcgtca tcctgatgtc gccgctgttc gggccgttca tttatctgat cgcgggcatc
    18721 aaccgcgtgc gccgggacca gatatcccag cagcgcgacc gcgcgatgaa ggactacacc
    18781 cggatcgaca ccccggcggt gcatgatgtc acgccttatt cgagcccgca attcgcgtcc
    18841 ctgcgcatac tgggcgaccg cgtcagccgt tttccgctgc acgacggcaa ccatattcag
    18901 attctcgccg gcggcgatga ggcctacccc gccatgatcg ccgccatcga cgcggcacgc
    18961 gagaccatcg gcctgcaaac gtacatcttc gacgacgacc cggagggccg gaagttcgtc
    19021 gaggcgctgg cccgcgcgca aagtcgcggt gtcgagatcc gcgtcctgat cgattcgatc
    19081 ggctccaagt actcccgccc gcccgtcata caccgcctgc atcgccatgg catacggacg
    19141 gcgctgttca tgaccaaccc gctgggtatg aaaatgccct acgccaacct gcgcagccac
    19201 cgcaagatcc tggtcgtcga cggccgcatc ggtttcacgg gtggaatgaa tatccgggcg
    19261 ggcttcgtgt ccgccgtggc gcgagacaaa acggccatgg atacgcattt tcgactggaa
    19321 ggccccgtgg tgctgcaact ggtctcggtg ttcgcgcacg actgggaatt caccacgcac
    19381 gagacgctac ctatgccggt ctggtgtcac gatgcctgga ccccgcccga gccgcgcgtg
    19441 cccgcccgct gtgtgcgctc gggtcccgac cgcgccatcg tcagcaccca caacatgctg
    19501 ctgggcgcgt tcgcggtcgc ccagaggcac atacgcatcc agtcgcccta tttcctgccg
    19561 gaccagatcc tgcttggcgc catcaacacc gcggcacgcc gcggcatacg ggtcgacgtc
    19621 gtcattcccg gggccaacaa cctgcgcctg gtcaactacg ccatgaccgc ccagctcgac
    19681 caaatcatcc gcaacggctg ccatgtctgg cgcgccgccg gcaacttcaa ccattccaaa
    19741 ctcatcacca tagacggcgc ctggtcctat gtcggctcgt ccaatcttga tccccgcagc
    19801 ctgcggctga acttcgaact cgacgtcgag atctacagcc gccacatggc ggccgacatc
    19861 gaacgcctca tcgacgccga gatccagggc gcgcaggcgg tcacgctcga gaacctggcg
    19921 aagacaccgt tccggaaacg cctgcgcaac aaaatcatct ggctcgccag cccttatcta
    19981 tagcgccaag gcacacgacc atcgcgccac gacgggcgtg gcacctcaaa agaaaggggc
    20041 tccgaaaccg gagccccttt ctacactgct tttgaaggaa ccactttagc tttcagctag
    20101 gtgttacttc gccttggcct tcggcgcggc cttcttgtgc gccttgtgat gcttggaagc
    20161 tttgtgcacc ttgtgggttt tgtgatgctt ggcggtcgtg gtcgtaccag cggcgaaagc
    20221 ggccggcgcg gcgacgaggc ccaggctcaa aaccatcgaa gacaaagcaa gagcaagacg
    20281 cgattgtttg gtcatttaag tgactcctga aacagtttta tgaggctggt tggacagccc
    20341 ggatgatgag ccgccccacg cgggttgacc catgcggagt atgagcgcgc cgggcttgct
    20401 aagttctggg ttgaagcctg acaagatcag ttaattgtaa taaattgatt cttccaagaa
    20461 acccatattc ccgcaaaaac agggagattt cagcagtttt ttgggctgta aataacatat
    20521 tgaaacaata ttgaatgaga gctgaccgaa cttccagccc gctgtgacaa atgccctatc
    20581 gatgacaccg gatcacacca acgttcggcc gggtgggttt ccacacccga acgcgcgcgg
    20641 ggatgctgaa accgatctga aaatcgatcg ggtcgggcac catcgccatg gcggtatcat
    20701 catggagcga ttcggcggcg cttcccgccg cgatgtgttc aatcacgaga ctggcgcgca
    20761 caaccattcc gccacaacag gagctgacaa aatgaaggcc gtaacgcctg acggatccga
    20821 caaagccacg acctggagag acgagacacg cgtcctgcac ggcgatcagg gcatggaccc
    20881 cggcagcgcg gtcgtggccc ccattcacta cagcgccacg ttccgggcgg aaacccctgc
    20941 ggaattcgcc gaaatggcca acacgccccg cccccgcgcc tactacacac gctatggtaa
    21001 tccggtgcac aaacgagtgg aaaaactcat cgccgacctg gagggaaccg aaacagccat
    21061 ggtgctgggt tcgggcatgg gcgccatcag caccaccgtc ctggcgctgg tcggccgggg
    21121 cgatcatgtg atcgcccaac ggcgccatta catgagcacc tcgcggcttt tcgaagaggt
    21181 gctgcccagg tttggcgtcg agaccaccct cgtcgaccag accgatgtgt cggcgttcgc
    21241 caaggccatg cgccccgaaa cccgcctcgt catgctggaa acaccggtca accccacgct
    21301 gtcactcacc gacctgggtg ccgtggccgc gctcgcccgg ccgcgcggcg tgctcaccat
    21361 cgccgacaac acattcgctt caccgctgaa ccagcagccc catgccctgg gcatcgacat
    21421 cgtggtgcac agcgccacaa aatatctggg cgggcatcac gacctcacgg ccggcgcggt
    21481 gtgctgcacg aatgaactgg ccgagcgcat ctgggacatg cacaccacgc tcggctcggt
    21541 tctttcaccc atggatgcgt ggctgctgtt gcgtggtttg cgcacgctgc cgctacgcgt
    21601 cgagcgcatc aacgccaacg ccctgggcct ggcgcaatgg ctggaacgcc aaccccaggt
    21661 cgaacgcgtg ttttaccccg ggctggccag ccacccccag catgatctgg cgcgcaacca
    21721 gatgagtggc ttcggcgcgg tcgttgcgtt ctcgattcga ggcgggtacg ccgccaccag
    21781 tcggttcgtg tccgcactca aactggccac ccacgcggtc agcctgggcg gcgttgaaac
    21841 cctcattgtg cacacagccg ccatgtgggc cggcaccatg accgaggcgc agatggcctc
    21901 cgcgggcata gagcccaatt tcgtgcgcat gtcggtcggc gtcgaacaca tcgacgactt
    21961 gaaagcggat ctggggaatg ccctgatgca agtcgattga ggaaccgcgc tgtgacccgg
    22021 gatttttacc cttccgcctc tggccatgaa tacctgcccg aggttgggcc gcgtggtcag
    22081 gcggcgaaat tttttcgacg ccacgcgaac accaacccca tgatcagcgc tggaatggcg
    22141 atcagcatga aagccggccg atagctgttg gccagcgatg cgatgcctgc aaagatcggg
    22201 ggcccgacca ccacccccag aaatgtggcc gccagggttc cgcccgtggc gatgctggtc
    22261 tgcccaggag gcgcctgacg cgccacctcg gccagataca caccgttcca gcccagtgcc
    22321 gccgaaccca gtgcgatgag caccaccacc aacaacgcgg gaggcgtgcc aaccaccaaa
    22381 ccagccagaa cccagcaaaa cacagccatg gccacaccca ggaccgtcag catggcgatc
    22441 gcgccgacga aacgatcggc acaccatccc cacagcacac gccccacgac ccccgccact
    22501 tgggacaccg acatcatgac gccggcggct atgagcgtgt agcccaggct gtcatgcaga
    22561 aaagtgacca gataagcggt gaagcacaat tgcacggcgg aaaaggtcag cgaacccaac
    22621 gacagcaacc gaagagcgga atgcctgatg atcacgcgca ccggttcgcg caggttaccc
    22681 agccccagag gcgaccccgc cgtccggtcg gcatcgagat ccttgcgtga aaactgcacc
    22741 cccaccgcgc tcacaatgca tgcgatgatc gccacgagta cggcgccgcg ccagccagtc
    22801 caggcgtcga gcctgggcag gaacagcccg gccagtacgc cgccaagcgg aacccccgtc
    22861 tgtttgatcg agaacaccaa tgacatgcgg tgcgtcggcg tggtcttgac cagtagatgc
    22921 gaactggcgg gcgtgacctg cccatatccg cacccaataa gcagcgcccc cgccgccgcc
    22981 agcaccggca ccgccgtcag gctgcaagcc aaccccagcg cgcacatcag cagactcccc
    23041 tggctgatgc gtatggcgcc gaaccgcgcc acgtagccac ccgccgccag gctgctgaac
    23101 atggcgccca cgtaaatcag cgcgacatac acgcccacca gctcccccgg cacgccaaac
    23161 actggtgcca gtgccggagc cagaacgggc acggtcagcg ccgccgccga caccagggac
    23221 tggatcgtca gcgtaatggc aagcaccttg cccatggtag gcatcatcgt agtcgtactc
    23281 gccaaaatgc gaggcctgcg gagcaggcct tgtgaaaaaa aaatccaggt ttcccaccga
    23341 acagtggaag tcgttcgacc gcaccattcg cggggccagc ataaccgaca gaggaaacga
    23401 cgtgcaaacg ctgagcaacc acaagttcca tgacgtggtg gtggctgcgg ccagcaatca
    23461 gatggcgcat cattccggta tcccggtcgc gtcaggcaga accttgtatg gcaaagcgtc
    23521 gcgttgcggc cgaccggggc aagtgcaaca atgcggattc cttccgcagc gcctccacgc
    23581 ggggccttcc ggtgtaattg cctcacgcgt cggcgcgtgc gaaagtcccg atgagcaaca
    23641 ctccattcag cttcaagaaa atcgctgtcc cggcatttgg cccctccctg ctgttcggaa
    23701 cggggaaagg cgccattctt cccgtcatcg cactcaccgc ccgcgacatg aacgcatcgg
    23761 tggccctggc cagcctgatc gtgggcctga tcggcatcgg ctcgctcgtc agcaatattc
    23821 ccgcggccat gatcatcgcc cgtcatggcg aacgggtatc catgatcggc gcggccgctg
    23881 tcagtatcgt cggcctgatc ctgtgtctgc tgccctcgac ggtctggctg ttggcgctgg
    23941 gcgtgctgct catcggcatg gcgcagtcgg tgtttttcct ggcgcggcaa acctacctga
    24001 tcgaggccgt gcccgttcac ctgcgcgcgc gcgccatgtc cacgcttggc ggaaccatgc
    24061 gcatcggggt gttcgtgggg ccgttcctcg gcgccgcgct aatgcacttc atgggaatcg
    24121 ccggagccta ctgggccgcc atcgtcatga tgctgggtac gggaatactg gcatctctca
    24181 ttcccgacat gggcagccag aagcgggaat cgaagggtgc cgcaagcggc gcccccggca
    24241 tggcgcgtct tgcccgaagc cacatcaagg ttttcgccac cgtgggagtc ggcatactgc
    24301 tggtgagtgg cctgcgggcc tcgcgtcaga tcatcatccc gctgtgggcc gatagccttg
    24361 ggttgggccc ggcatccaca tccatcatct tcggcctggt cgccgccata gacatgctgg
    24421 cgttctaccc ggctggcaag gccatggacc agcgcggccg gcgctcggtc gccgtgccat
    24481 ccaccatcat catgggcctg tcactgatcc tgatcccggc cacgaatggc ctgatcacct
    24541 ttgtcatcgt ctccatgctg atgggcctgg gcaacggcat cgggtccggc ctcgtcatga
    24601 cgctgggggc cgacgcctcc cccagcgcgg gccgcaccca gttcctggga atctggcgcc
    24661 tcatctccga catcggggcc agcggcgcac cggtcctgtt gtcgatcatc accgctgcgg
    24721 cgtcactcgc ggtggcttcg atcgccgcgg gcgtcatggg gttcatggcc gccggcatat
    24781 tcctgcactg gctgcccagc acggggcggt cacgcacacc ctgacaccga cccaaccaag
    24841 gaatcgcaca tggcccatgt cacagtgatc accacgggag gcaccatcgc ctcgacccgc
    24901 gcatcggcgg acggtcccgt cacggccaac gtgtcgggca acgacttgct ggcatcgttg
    24961 cacggcacac ccatcggcat cgacgtcact gtcgaggagt tctgcaacat cggcagcaac
    25021 gcgctggatc tgccaacggt tcatgcgctg ttggcccgta ttcgcgcggc gcttgccaaa
    25081 ccagactgca ccggcgtggt cgtcacccac ggcacagaca ccatggagga atcggcgttc
    25141 ctctccgact tgctggtcga cagttcaaaa cccgtggtgt tcaccggcgc ccagcgccac
    25201 gccagcgaac ccgataccga tggcccgcgc aacattcgcg atgcgctgat agtcgccgcg
    25261 gcaccgaacg cccgcgagct tggcacgctg gttcttttcg agggcgacgt gcatgccgcg
    25321 cgcgacgtca ccaagaccca cgcatcgcgt gtcgatacct tccgttctcc gggcctagga
    25381 aaactgggcg aggtcgatca gggcgtattt cacttgtatc gtcgcccggc acagcggcgc
    25441 acactggccg atcacgggct cgacgaacac gtcgagctcg tcaaactggg gctgggagtc
    25501 aaacccactt acctggaatg gtgcgcggac aatggcgtgc acggggtcgt gctggaagcc
    25561 ttcggccgcg gcaacgctcc gaaaggcttc gccgcggcga tcgcacgact tgtctcggca
    25621 aggacgccgg tgatcgtcgc ctcgcgctgc ccggaaggac agaccgccct catctatggc
    25681 gcggacggcg gcgctgtcac gttggctgaa gccggcgcgg tgttcgccgg ggatctgtcc
    25741 ggcgtcaagg cgcgcctgat gctgtgcgtc ttgctggggg ctggtgccac actgcccgag
    25801 attcgcgccg ccttcgaacc agcctgatcc acgacaaacc ggccctgacg gcctgaccag
    25861 ccgcgcatcg ggctactttg gttcttcggc gcaaaaagtg gtcagacctt ttgacaccat
    25921 tgcgggtgga ggttattctt cctctcggag gtagggacaa aaagggccgg cacgcaagat
    25981 gccctcgcca gttgtatctg tcgtcattcc aactctcagg aggagattat gtcggacatg
    26041 gaacatggcc agcgcgcggg gatcacccgg cgcggcgtac tcaaagtcgg ggcagcgggg
    26101 ttactgaccg gtatggcggg cgccagcccg ttcattcgtt cggccctggc cgcagagccc
    26161 ataaaactcg gtgtgctgct ccccaaatcc ggtacctacg ccgtacaggg cgatcaaggc
    26221 cacaacggat ccgtgatcgc agttgaagac gtcaagggcg aagtgctcgg tcgccctgtg
    26281 caactggtat ggctggacga aaacggcccc cagaccactc agcagaacat gcaccggctg
    26341 atccagcagg agaaagtcgt ctccgtttcg gggggcgtat caagtggtga catcctggcc
    26401 atcatgccag tggccgagcg cgagaaaatc ctgctcatgg ccacggggcc caacgccacg
    26461 gaaatcaccg ggtccaaatg caatcgctat acattccggg tcgatctacc caactacgaa
    26521 accgtgcgca gcgtctatcc cacgctggaa aaacacggca agaactggta tttcctgtac
    26581 gcttcttacg cctggggcat agacggctat aaccagatga gcctgctgct caagcagggc
    26641 ggcggcaaaa tcctgggcgc cgaccaaact cccttgggca ccactgattt cagttcatac
    26701 gtcctcaaga tcatggccgc caagcccgat gtcattttcc ttggcctcgg tggcggcgac
    26761 atgaccaact tcctcaagca gttccatcaa atgggcctga ccggcaagat cccggtgtca
    26821 tccctcattt gcaacgatac cgatatgtgg gcggcaggcc ccgaggcagc caccggcctg
    26881 tacccgaaaa tctggaacta taccggggca cagaacaccg aggccagtca cgcatttgcc
    26941 aagaagtacc aggccaagtt cggcacacca cccgaatccg aagcctggca ggactggttc
    27001 ggcaccacgg ccatacttac cgccatcaag gaaacgggct ccaccgacac gcaaaagctc
    27061 atcgatttcc tggaaagtca caaattcgag ggctacaaga aagaagccat gtacttccgc
    27121 tcgtgggatc atcagttgat ccagcccaca ctggtggcag aggtcaaacc caagatcacc
    27181 accaagtacg actacttcaa catcgtcgcc gtcgcaccag gcgagggcaa gccgctcgaa
    27241 agcctgtacg gttcgaaaag cgaatccgaa tgcaaaatgt gaccgtcgtt attctgtgac
    27301 cgccgtcact ccccgaagca ggtacacgcg cgccatccgg acaatccatc caaccggatg
    27361 gcgcgccgca taaaaagata aaggagatgc ttagtgataa acgccacact tttcgcttcg
    27421 cagctcatga acggcctggt cctgggcttc ctgttcgtcc tcattgcggt cggcatgtcc
    27481 atcatcttcg gcatgctggg gctggtgaat ttcgcgcacg gagccttttt cgccctgggg
    27541 gcgtacttcg ctctgtcgct tcggccgtac gtcggtttcg gtggtgtgtt ggttctcgcc
    27601 cccatactcg tgggcatcat cggactcgcc attgaacgca cgctgttgcg tccactctac
    27661 gacaaagagc ctctgctcag cttgttgctc acgttcggcc tggcgctgtt catcgaagaa
    27721 ctgatccgca cgctctgggg ctccatgggg catccgttcg ataccccggc ctttctcgcc
    27781 ggcttcttcc actacggccc ggtgtttgtc acacgctatc gactggccgt gctggtgatc
    27841 accgtcgtga tcctcgccgc catctggctc tttctcgaaa aaacccccta cggccgcatc
    27901 gtccgggctg gttcacgcga cccggaaatg gtcagcatgc tgggcatcaa cctgaagcgg
    27961 gtattcatgt tcattttcgc tctggggtgc cttctggcgg gggcggctgg cgtgctggcg
    28021 gcgccgctct ggagcgtcat gccgtcaatg gccgaacagg ccatcatgcc ggccttcgtc
    28081 gtcgtggtca tcggtgggct cggtagcctg cgcggcgcca tcgtggccgg cctcatggtc
    28141 ggcatcgccg tggccatgac catccagttc tggcccgtgg cctccacctc catcatgtat
    28201 gtgctcatga tcatcgtgct gctgttgcgg ccgcgcgggc tgttcggcga acactgggag
    28261 cgcttcgaat gaatcggcca cgaagcgccc tcacccatcc gctcgtggtg ctcgtggcgg
    28321 tattcatcat catgccctgg ttgatgaccc tggcgggctc cactatcagc ctggccaccc
    28381 aggtcgtcat ctacacactc tatgccctgg cctacaacct gctgctaggc tatgccggca
    28441 tggtgtcgtt cggctcatcg gtgttcttcg gcatggccag ctacgccagc gcgctgttcg
    28501 ccatccatgt ctaccagaac gtcgccctgg cactgatcgt tggcacggcg ttcgcagccc
    28561 tgctggggct ggttctaggc atgctgattt tgcgccgacg cggcctgtat ttcgccctgc
    28621 tgacgctggc gttcacgcaa ctcttctacg aaatctgctt ccgctggacc agcctgacag
    28681 gcggagagaa cgggctacag ggggtgctgc gcccgggttt ggaaaatgcc tggcactact
    28741 atgttttctg cgccatcata gtggccatct gcgcctggct gctctatcgc atcgcgcatt
    28801 cacctttcgg gcgcgtgctg caggccatcc gcgacaatga aaagcgggtc cagtacctgg
    28861 gctacaaccc ctggggatac aagctcagcg cgctggtgct gcacgcagcg ttcatcgggc
    28921 tcggcggcag cctgctgtcg ctgctcatcc acggggtgta cgccgacaac atgaactggc
    28981 agcatgccgg cgacccggtc atgatgacgc tgctgggcgg catccaccat ttcctggggc
    29041 cgctgtgggg cgccatcatc tacatcaacc tcagcgacca gctcagttcg ttcaccgagc
    29101 actggtggct gttcttcggc gccctcataa ttattgtggt gctgttgtca ccggaaggcc
    29161 taagtggtgt atatgcccgc ttgcgtggcc acgaccgctg ggggctcacg cgcacaccaa
    29221 cgcctgccat gcccccctct ctgcccgatc cattcgcggc ccggggggcc acttacgaag
    29281 gggccatcct cgaagtacgc catctcagca aacgcttcgg acgcgtcgtc accgccgacg
    29341 atatcagtct gcaggtgcgg gccggcacca tccacagcct gatcggcccg aacggcgccg
    29401 gcaagaccac gctgttcaac atgctcaccg gcctcatccc caatgacagc gggcaaatcc
    29461 tgttcaaagg gggggacatc agcaagatgg cggtgcacga acgcacccgt ctgggcatgt
    29521 cgcgctcgtt ccagatcgtg agcctgcccg ccaatctcac ggtttttgaa accgtgcgca
    29581 tcgctgtgca ggccggctcg ccactgcgcg catcgctgtg gcgcaacgcc tacgacctga
    29641 ccgacctggt cgatcggacc tgggcgttgc tgcacgccgt gggattggaa gacaaggcgg
    29701 gtgaaaccac catcaacctg ccgcacggcg aacagcgcct gctggacatc gccgtgacca
    29761 tggcgacggg ctcggaactt ctgttgctcg acgaacctct cgccgggctg gcggacgccg
    29821 accgcgaacg catcggcacg ctcatccgcc agcttgccgg caagtacacc atcctgctca
    29881 tcgagcacga catcgaccgc gtcatctcgc tgtccgaccg catcaccgtg ctgcaccagg
    29941 ggcacctcat cgccgacggc gacccccact cggtcgtcaa taacccgcaa gtcgtcgaag
    30001 cctatctggg ggaagccatc aatgacgacg gaaaatcggc gccatccact ctcgccgctg
    30061 atggcgtcac acgcgacatc ggcaagacta tcctgcaatt gcgcgacgtg gcctccggct
    30121 acggcggcag ccatgtgctc gatggcctat cgctggaagt gcgcgaaggc gaggcagtcg
    30181 ccctgcttgg ccgcaatggg gtcggcaaga ccaccactct gcacaccatc atgggccaga
    30241 tcgcggtcaa cgatgggcac atccggttca acgacacaga catcaccggc tggccatcca
    30301 atcgcgtcaa ccggcagggg ctggccattg tcccgcaagg gcggcgcata tttcccaacc
    30361 tgagcgtcgt cgacaatctg ctcatcgccc ggcgaccggg cggctgggag cccaaccagg
    30421 tgtacgactt gttcccgaag ctgggcacat tgcgggacgc catcggcggc aatctcagtg
    30481 gtggagaatt acagatgctc gcgatcgctc gcgcgctcat ggcccccacg caattgattc
    30541 tgctggacga gcccttcgaa ggcctggcgc ccgccatcgt caacgaggta ctcgacgccg
    30601 tcatccagct gcgccagcgc acgagtatcc ttcttgtgga acagcgtgtc gacctcgccc
    30661 tgcgtatggt cgatcgtgcc tacatcatgg tcaacggccg aatcgcctat gaaggcgcgg
    30721 cggccgagct caaagagaac aaggcgctgc aagtcaagct tctgggtgtg tgatgcgaca
    30781 aacactattg acgggcgtct cgatcgtgcc gccaacaatg aagaaggcat gtctttcata
    30841 ttcacagcac gctatgaaac gatgggcacc gcgacacctg gcgccagaga gccggcgcgc
    30901 gcaaacgcgg gtggctccca tatgccaacc gacttcaacc gaggaagaca atgtttgacg
    30961 aaacaagcat gaaagacaga aaagtgctga tcacagccgg cgccagcgga ctagggctgg
    31021 aaatggccaa ggcattcact gcggcgggcg ccacggtgct ggtctgcgac gtggaccaaa
    31081 acgccttgga ggcggcgagc gcggccctac ccgggctgca caccgccgtc gctgatgtat
    31141 cggacgaagc cagtgtcgcg gcactgttcc agcaagtcga cacccagctc ggtggcctgg
    31201 acgtcctggt caacaacgcc ggcatcgccg gcccgaccgg ctatgtggag acccttagca
    31261 agatcgactg ggaccgcact ctggcagtca acatcaccgg ccagttcctg tgcgcacggc
    31321 tagccgtgcc gctgctcaag cagtcaaagg ccggcgtcat gatcaacctg tcatcggccg
    31381 ccggacacct ggggtttgcc ggccgttcgg cctattcggc ctcgaaatgg gccgtggtgg
    31441 ggttcaccaa gacattggcc atagagctcg ggaagttcgg cgtgcgcgtc aacgccatcc
    31501 tgcccggtgc cgtcgaaggc cctcgcatac gcgccgtgat cgcggccaaa gcacagacac
    31561 tgggcgtccc ggtcgacgaa gtcgcgcatt catatgaaag ccaggcctca ctcggacaca
    31621 tggtctcggc gcgcgacatc gccaatatgg ccctgttcgc cgccagcgac gccgccgcca
    31681 gcgtcaacgg ccaggcactg gtggtcgacg gccacacaca agcgctggtt tgacatgccg
    31741 aacgacacca tgaacatcgc catcattggc tccgggctca tcggccaggc ctgggccatc
    31801 gttttcgctc gcgccgggtg ccaggtccgg ctttgggacg gggattcagc cgcgctgaat
    31861 cgtgccgtcg agctcatcgc gcgccaggcg cgcgaactgc agaagcaacg actgctcgat
    31921 gacgcagacg ccctgacgac ccgggtacag gcctgcgacg acctgggcgc ggcgctgaag
    31981 ggcgccgcct atgcgcagga aaacctgccc gaaacactca gcatcaagaa agacatcttc
    32041 aaacgactcg atacccttgc cgcacccgac acggtgctgg ccagctccac ctccagcatc
    32101 ccagcctcgg ccttcacaga agatcttccc gggcgcggcc gctgtctggt tgcccacccg
    32161 gtgaatccgc cctatctcgt tcccgtggtc gaactctgcg gcgctccctg gacggacgcg
    32221 ggtgcggtgg aaaccgcgcg cgccatcatg caaaccgtgg ggcagaaacc ggtggtggtt
    32281 cgcagagaac tggaagggtt cattctcaac cgtctacagg gtgccttgtt gcgcgaggcg
    32341 tttcgcctgg tgcagaacgg ttacgtgagc gttgaggacc tggacgccac cgtcaaggat
    32401 ggcctgggtt tgcgctggtc gttcatgggg ccgttcgaaa ccatagatct gaatgcgccc
    32461 aacggcctgg ctgattactg cgggcgctac ggcggcatgt atgagtccat cgccaaagaa
    32521 cagacggata ccgatgcctg gcccgccggc ctgataaacg aaatcgagca acaacgacgc
    32581 gcgcttctgc ccggctccgg gctgctggac aggcgccagt ggcgagacca gcggctaatg
    32641 gcgctgctgc ggcacaagaa ggaaatggaa acgcccgaca cctgagcccc ttccatgccg
    32701 gccatggttt gccaacagcc agcccgacac aaatccactc tctattcaac aaggaagaat
    32761 catggcaaag aaacacaaag ccatcatcac gtgcgccgtc accggcgcca tccacacgcc
    32821 cagcatgtcg aagtacctgc cggtcacggc cgacgagatc gccgacgccg gcatcgccgc
    32881 agcgaaggcg ggagcggcca tcctgcatct gcacgcgcgc gaccccaagg acggccaccc
    32941 atcccaggat ccggagctgt tccgcccatt cctgaagaag atcaaggcca acaccgatgc
    33001 ggtgatcaac atcaccaccg gcggcagccc acacatgacc gttgaggagc gtatgcgccc
    33061 ggccatgacc ttcaagcccg aagtcgcctc gttgaacatg ggctccatga acttcggcct
    33121 gttccccatg ctcgaccgct tcaaggaatt caagcacgac tgggaacgcc agcacctgga
    33181 aaacagtcgc gacctgatct tcaagaacac ctacaaggac atcgagacca tcctgcgcaa
    33241 gggcaccgaa aacggcacgc gcttcgagtt cgagtgctac gacatcagtc acctgtacaa
    33301 cttgaagcac ttcctcgatc acggcctggt gcaaagcccg ccattcatcc agtctgtgtt
    33361 cggcatcctc ggcggcatcg gccccgaccc ggaagacctg atgcacatgc ggcgcaccgc
    33421 caaccatctg ttcggcgaca actacgaatg gtccatcctg ggtgccggcc gcaaccagat
    33481 ccccctggcc accatgggcg ccgccatggg gtccaacgtg cgcgtggggc tggaagattc
    33541 gctgtggata ggcccgggga aaatggccgc ttccaacgcc gaacaggtca gccgcattcg
    33601 cgtcatcctc gaggccctca acatcgaggt cgccacacca gacgaagccc gtgaaacatt
    33661 gcacctgaag ggtggcgaca aagtcgattt ctaggcctcg ccgacacaca agccgtcgac
    33721 acatggaaaa cgaaggagca gcatgtttga tcttaccggc caggtcgcct tggtcaccgg
    33781 ctcatcgcgc ggcattggct atgcagccgc ccaggccctg ggccggcaag gagccactgt
    33841 cgccctcaat ggccgcgacc cggacacctt ggaaaacgcc gccaatgacc tgcgcagcca
    33901 gggtatcacc gtgcatacgg cgcccttcga catcgccgac gtggcccagg ccacgcatgc
    33961 cgtcgacgcc ctgcaaaagg ccacgggaaa aatagacatc ttcttcgcca acgccggcat
    34021 ccagcaccgg gagcccttgc tgtcgtttcc ccaagtggaa ttcgagcgca tcgtcttcgc
    34081 caatctgacc gcccaatggg cactgggccg atacatcgcc gcgggcatga gcgaacgcgg
    34141 ctacggacgc atcttgttca cgggttcgat caccgccttg ctgggtcgaa aggaaatcac
    34201 cgcctatacg gccgccaaag cggccatcca tggcctggta cggcagtggg ccacggaact
    34261 gtctgtgtcg ggcgtgaccg tcaatgccat cgcaccgggc tatatccgga ccgaactcac
    34321 ccgcaatctt tgggccgacg aggagttcaa ccattggctg gaaggtcggg ttccccagaa
    34381 aaaatggggc aaccccgaag acatcgccac cgccgtcgta ttcctcgcgg cgcgagaatc
    34441 ggctttcgtt accgggcaag tactcgtcgt ggatggaggc atgtcgtcgg tgatgtaagg
    34501 atttcacacc accagataaa tcaggaaagg cacgcttctt ccaaagccgt tttcatccgg
    34561 cccgctattt cgattgatcg tggcgcggca ggagggtctc gaacccccga cctacggctt
    34621 agaaggccgt tgctctatcc aactgagcta ctgccgcgct gcttgagcac ggcattttac
    34681 cttgccacgc cgtttacatc ccgttgcgtc ggtatcgacc cataggagga aaattccaac
    34741 g
//
LOCUS       DASTNK010000003         7206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_373335, whole genome shotgun sequence.
ACCESSION   DASTNK010000003 DASTNK010000000
VERSION     DASTNK010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7206
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373335"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFR20_00240"
     CDS             <1..353
                     /locus_tag="VFR20_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HEU0228973.1"
                     /translation="TASTHRTMTPTILRQYQTIADISSKMLDLARTDKWDQVVELGHA
                     YHSAVETLRGLDDLGHEDRSARRSLLTRILDDDANIRRLASPELRRLEALLGNMKRQQ
                     DVLRTYCAWSTPNQ"
     gene            350..1732
                     /locus_tag="VFR20_00245"
     CDS             350..1732
                     /locus_tag="VFR20_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0228974.1"
                     /translation="MSIGGPSALGTLLVQRLDAVLGTTLSQQTNLISGARPDAVSQPA
                     DADKPEPGRDQLVRHPRESVDRSQGRIQGDSRSAIEKPTSTSQRQDPTGRLATDVEST
                     TSAPTRLGFAARTILALLAEFPDQAPPVATDTALFDPAEARNGPHASAAGSGVATGST
                     TGPKAASTPGSGSTTALGTRGAAPGAHPASETGSAPHPSPEGRVTGTGKSDEAISTPT
                     GMAPALARALNRAVEQSGLFYESHLADLAFGRRDANAVSAEPQARLPPSANDASTGSS
                     GAAKAVASSAAPPQPGATPSSAAQIPGMPSLAGHPPSAAPAMSSPMVVQATAFGLPTE
                     AHLLVRQQLETLANQAFAWQGPAWPGAPMHWTVERHQHSTPHDDTDTPAYWATHVRIT
                     LPRLGLVDASLTLLAGSRIAVRLGTHDGSPTLEGHVADLRSRLLAAGLAPGQIIIDSH
                     ASRSGQRSRP"
     gene            1729..2040
                     /locus_tag="VFR20_00250"
     CDS             1729..2040
                     /locus_tag="VFR20_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEU0228975.1"
                     /translation="MTPDSSHPDNPAHTGPGNARPRAVAIRYDTDEPAPRIVAKGYGT
                     LAETILRTAREHGLYIHESPELVGLLMQVDLDRHIPPELYRAVAELLAWLYALEKAEQ
                     A"
     gene            complement(2045..2371)
                     /gene="fliE"
                     /locus_tag="VFR20_00255"
     CDS             complement(2045..2371)
                     /gene="fliE"
                     /locus_tag="VFR20_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEU0228976.1"
                     /translation="MEQLSGIESMLAQMRAAARAAASGPTRPDAVGGAGGGFAAELER
                     SLQHVSGMQNQANSQARAFELGVPGVSINNVMIDLQKANIAFQATVQVRNRLVAAYQE
                     IASMPI"
     gene            2793..4487
                     /gene="fliF"
                     /locus_tag="VFR20_00260"
     CDS             2793..4487
                     /gene="fliF"
                     /locus_tag="VFR20_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEU0228977.1"
                     /translation="MTQQSSLLTRFPALAKVGNWPKPMQLGALAAVIALVIVLALWSQ
                     GPDYRVLFSNVDDQDGGAIVTALGQMNVPYQFSDNGRTILVPADKVYRTRMQLASQGL
                     PRSGEAGFELLDKTRFGASQFTEQITYQRALEGELARSIEAINAIKKARVHLAIPHQS
                     LFVRDRQTPTASVMVTLYPGRALSPSQVSAVTWLVSSSVPNLPADHVSIVDENGHLLT
                     APTGEAGDDATRRTLAADIEQRTVQRVLSILTPLVGTGNVRAQATADVDFTQREQTSE
                     TYGPNQEPGQAAVRSKQLSASTQNGVNPPEGVPGALTNQAPANATANIDAPRVNNRGR
                     RDTTRDRRTATPARQPNTPQSAQAAQEAASQGPTSARHDETINYEVDHTIDHVKNPVG
                     TLRRLSVAVVVNYHDGKGGKPEPLGQSEMAKINELVRQAVGFSTARGDTISVVNSPFS
                     ANTQDQLPPWKDPYYLDLAMQAGRYLLVLIVILLIWRRVLKPLARNAAESWQKPLPQE
                     AGGGAAARDDDEAARKRESEINRYEDNLNVARTMADKDPRAVAMVLRSWMEKHGSH"
     gene            4474..5478
                     /gene="fliG"
                     /locus_tag="VFR20_00265"
     CDS             4474..5478
                     /gene="fliG"
                     /locus_tag="VFR20_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEU0228978.1"
                     /translation="MAATDLDNLDRCAILMMSLGEDAAASVFKYLSPREVQKIGAAMA
                     SLKQVTRRDVDAVLEGFRQEADQFMAVTLGSGDYIRSVLTKALGTDRAAGLIEDILEA
                     GDGDNGIDALNWLDPNSVAELICDEHPQIIATILVHLERDRASGVLALLPERLRNDVM
                     LRIATFGGVQPAALHELTDVLNNMLSGQGAKRSKMGGVRAAAEILNFMNSADEEAVVA
                     NLREQDADLTQRIVDEMFVFDNLADIEDKSIQMILKEVDTTSLTIALKGASEDLREKF
                     FRNMSARAAEILRDDLDAQGPVRMSRVEQEQKAILQIARKLAEAGQITLDNPGNDEYV
                     "
     gene            5471..6232
                     /gene="fliH"
                     /locus_tag="VFR20_00270"
     CDS             5471..6232
                     /gene="fliH"
                     /locus_tag="VFR20_00270"
                     /inference="COORDINATES: protein motif:HMM:NF018358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HEU0228979.1"
                     /translation="MSDETALDVAAARARWRRWKMSGFDTAANAIVDGQAPAHRAEEL
                     AARVQQARLAAYEQARVEGHGLGHAEGLRSGLDEGRAAGREEGYQAGYEAGHADGRAQ
                     GAEQAAAEATRLAGLAGACAQSLAALENEVGAALASLSLRIAEHIVRGALDIEPERIV
                     DLVRQVLGNRDTDQDVSLTLHVHPSDLELVRHYMKNDHPGDKWRLCAVESMTPGGCLI
                     HSSLGAVDATLETRWHNVSAALGVLPPPGQSPRGT"
     gene            6264..>7206
                     /locus_tag="VFR20_00275"
     CDS             6264..>7206
                     /locus_tag="VFR20_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HEU0228980.1"
                     /translation="MRLAADARRIPLVPPFTASGRVTRATGLVIEATGLRMAVGAACR
                     IEIAPGSERWADAEVVGFHGQVLYLMPQSDISGLPPGARVLPAEPDKPVPGNATTQDR
                     RAMSAQTRRLPVGETLLGRVVDGSGNPLDGLGPLRHAHMAPLTARAINPLARAPIDTV
                     LDVGVRAVNGLLTVGRGQRMGLFAGSGVGKSVLLGMMARYTAADIIVVGLIGERGREV
                     KEFIEHNLGPEGLRRSVVVAAPADVSPLLRLQGAVYATRLAEYFRDMGRHVLLIMDSL
                     TRFAMAQREIALAIGEPPATKGYPPSVFAKLPALVERA"
BASE COUNT         1395 a         2511 c         2212 g         1088 t
ORIGIN      
        1 ccaccgcctc cacacaccgg accatgaccc ccacgatcct gcgccagtac cagaccatcg
       61 ccgacatcag cagcaagatg ctggatctgg cccgcaccga caaatgggac caggtcgttg
      121 agctgggcca tgcctaccat agcgcagtcg aaacgttgcg cggcctcgat gaccttggcc
      181 acgaggaccg ctcggcgcgc agaagcctgc tgacaagaat cctcgacgat gacgccaaca
      241 tacgcaggct ggcaagcccc gaactccgtc gtctggaggc attgctgggc aacatgaaac
      301 gccagcagga cgtcttgcgc acatactgcg cctggtccac accgaatcaa tgagcatcgg
      361 ggggccttcg gcactcggca cgctgctggt ccagcgactc gacgcggtgc tcggcaccac
      421 gctttcacag cagaccaacc tgatctcggg cgcgcgcccc gacgcggtca gccaaccggc
      481 cgatgccgac aagcccgagc ctggccgaga ccagttggtg cggcatccgc gcgagtcggt
      541 ggaccggagc cagggccgta ttcagggtga tagccgaagc gccattgaaa agcccacctc
      601 cacgtcgcag cggcaggatc ccaccggacg cttggccacc gacgtcgaga gcaccacatc
      661 ggcccctacc cgcctgggct tcgccgcccg aaccattctt gcgttgctgg cggagtttcc
      721 cgatcaggcc ccacccgtgg ccaccgacac cgcgctgttc gatcccgccg aggctagaaa
      781 tggtccgcac gcgagcgctg cggggtcagg cgtcgccacc ggttcgacaa ctggcccgaa
      841 ggcggccagc acgccaggca gcggatcaac caccgccctc ggcacacggg gtgcggcacc
      901 gggggcgcac cccgcctccg aaaccggttc agcgccccat ccctcgccag aaggacgagt
      961 gaccggcacc ggaaaatcgg atgaggccat ttcaacgccg accgggatgg ctccagcact
     1021 ggcccgcgcg ctcaaccgcg ccgtggaaca aagtggtctg ttctacgagt cccatctggc
     1081 cgacctcgcc tttggcaggc gtgacgccaa tgcggtgtcc gccgagcccc aggccaggct
     1141 gccgccaagc gccaatgacg catcgaccgg atcgtcaggc gccgcaaagg ccgtcgcatc
     1201 aagcgcggca ccgccccaac ccggcgccac gccgtcctcc gcggcacaga tcccgggcat
     1261 gccgtccctt gcgggccatc cgccttcggc cgcgccagcg atgtcgagtc ccatggtggt
     1321 acaggcgaca gctttcggcc tgcccaccga ggcacatctg ctggtgcgcc aacaactcga
     1381 aaccctggcc aatcaggcgt tcgcctggca aggaccggcc tggcctggcg cccccatgca
     1441 ctggacggtc gaacggcacc agcattcaac gccccacgac gacaccgaca cccctgccta
     1501 ttgggccacg catgtccgca tcacgttgcc tagactgggg ctggtcgatg ccagcctgac
     1561 gctgttggcg ggctcacgaa tcgccgtgcg cctgggcacg cacgacggtt cgccgacact
     1621 ggaaggccat gtggcggacc tgcgttcccg tctgctggcc gctggtcttg cgcccggcca
     1681 gatcatcatc gacagccatg cgtcccgctc cgggcaacgc tcccgaccat gacgcctgat
     1741 tcctcccacc ccgacaaccc cgcgcacacc ggccccggaa acgcacgccc ccgcgccgtg
     1801 gctatccgat acgatacgga cgaaccggcg cctcgcattg tggcgaaagg gtacggaaca
     1861 ttggctgaaa ccatcctgcg cacggctcgg gaacatgggc tgtacattca cgaatcgccg
     1921 gaactggtgg gtctgctgat gcaagtcgac ctggaccgcc atattccgcc cgagctgtat
     1981 cgggcagtag ccgaactgct ggcctggctg tacgccctgg agaaggcgga gcaggcctga
     2041 gggatcagat gggcatgctg gcgatttcct gataggcggc caccaaacgg ttacgcacct
     2101 ggaccgtcgc ctgaaaagcg atattcgcct tctgtaagtc gatcatcacg ttgttgatcg
     2161 acacgccggg cacgccaagc tcgaacgcac gggcctggct attggcttga ttctgcatgc
     2221 ccgacacatg ctgtagcgac cgttcgagtt cagcggcgaa accgccaccg gcgccgccca
     2281 ccgcgtcggg acgcgtcggg ccggacgccg cggcacgtgc cgcggcgcgc atttgcgcca
     2341 gcatgctttc aattcccgag agttgttcca tggttcaggt ttccggcgac cggacccgcc
     2401 ctgagccacg atggtggact cagcccggga caatggccgc attcatcaat gtctcacact
     2461 gtagcgcgcg acatggaccg ccagcgtgcg caactacgcc cgctttcccc ctcatttcca
     2521 ctctttccgg aagccgggga cgacacgccg gttccttgac gcggggtcgg agcgggacag
     2581 cgcgaggcca caggcggcca tcgcggcagt cacccggcat aaggcgacaa gtacaaccga
     2641 aaagcgctgc ttttcccggg atgacaaaaa aacgtttaca gcaataattc aggctcatcc
     2701 agttttgcga cctgttgcag catgtctatt ccgttcaagc accgcgccgg tggcgagcca
     2761 attcacggtc gacgcgcccg ccgaggccgc cgatgactca acaatcctcg ttgttgactc
     2821 gctttccggc cctggccaaa gtgggcaact ggccaaaacc catgcaactg ggggcgctgg
     2881 cggcggtcat cgcccttgtc atcgtgctgg cgttgtggag ccagggaccg gactaccgcg
     2941 tcctgttctc gaatgtcgac gaccaggacg gtggagcgat cgtcacggct ctgggccaga
     3001 tgaacgtgcc ctaccaattc tctgacaacg gcagaaccat tctcgtaccc gccgacaaag
     3061 tctatcgcac gcgcatgcag ctcgcctcgc aaggcctgcc ccgcagcgga gaagccggat
     3121 tcgaactcct ggacaaaacc cgtttcgggg ccagccagtt cacagaacag atcacatacc
     3181 agcgcgcact cgaaggcgaa ctggcacgct ccattgaagc catcaatgcc atcaagaagg
     3241 cccgcgtcca cttggccata ccgcaccagt ccctgttcgt gcgcgatcga cagactccga
     3301 cggcatcggt catggttacc ctttacccag gacgcgcgct gagcccatca caggtttcgg
     3361 cggtcacctg gctggtctcg tccagtgtcc ccaatctacc ggccgatcac gtatccatcg
     3421 tggatgagaa cggacatctt ctcaccgccc ccaccggcga ggcgggggac gacgcgacac
     3481 gacgcactct tgccgccgac atcgagcagc gcaccgtgca gcgtgtgctc tccatcctca
     3541 ccccgttggt cggcaccggc aacgtgcgcg cgcaggcaac ggccgatgtg gacttcacgc
     3601 agcgcgaaca gacctcggaa acctacggtc cgaaccagga acccggccaa gcggcggtgc
     3661 gcagcaaaca gctcagcgcg tcgacacaaa acggcgtgaa tccgccagaa ggcgttccag
     3721 gcgccctcac caaccaggcc ccggccaacg cgacggccaa tatcgacgca ccgcgcgtca
     3781 acaatcgcgg ccgtcgcgac accacgcgtg accggcggac cgcgacgccc gctcgccagc
     3841 ccaacacgcc gcaatccgct caggccgcgc aagaagcggc ctcccagggg cccaccagcg
     3901 cccgccatga cgaaaccatc aactacgagg tcgaccacac catcgaccac gtcaaaaatc
     3961 cggtgggaac cctgcgtcga ctatccgtgg cagtcgtcgt gaactaccac gacggcaaag
     4021 gcggcaaacc agaaccgctg ggccagtccg aaatggccaa gatcaacgaa ctggtccggc
     4081 aggccgtggg gttctcgacg gcacgcgggg acacgatcag cgtggtcaac agcccgttca
     4141 gcgcaaacac gcaggaccag ctcccgccct ggaaggaccc ttactacctg gatctggcca
     4201 tgcaggccgg gcgctacctg ctggtattga tcgtgatact gctgatctgg cgccgtgtgc
     4261 tcaagcccct ggcgcgcaac gccgccgaat cctggcagaa acccttgcca caggaggcgg
     4321 gtggcggcgc cgccgcccgc gacgacgacg aagccgcccg caagcgcgaa tcggagatca
     4381 accgctatga ggacaatctg aacgtggccc gaaccatggc tgacaaagac ccccgcgccg
     4441 tggccatggt gttgcgctcc tggatggaaa aacatggcag ccactgatct cgacaacctc
     4501 gaccgttgcg ccatcctcat gatgtcgttg ggtgaagatg ccgcggccag cgttttcaag
     4561 tacttgtcgc cgcgcgaagt gcagaagatc ggggcggcca tggcctcgct caagcaagtc
     4621 acccgccgtg acgtcgacgc cgtgctcgag ggtttccgcc aggaagccga tcagttcatg
     4681 gcggtgaccc tgggttcggg agactacatc cgctcggtgc tgaccaaggc cctgggcacc
     4741 gaccgggcgg cggggctgat cgaagacatt ctcgaggctg gcgatggcga caacggcatc
     4801 gacgcattga attggctcga tcccaacagc gtggccgaac tgatctgcga cgaacacccg
     4861 cagatcatcg ccaccattct ggtgcacctg gagcgcgacc gcgcgtccgg cgtgctcgcc
     4921 ctgctgccgg agcgcctgcg caacgacgtc atgctgcgca tcgccacgtt cggcggcgtc
     4981 cagccggccg ccctgcacga actgaccgac gtgctcaaca acatgttgtc cggacaaggc
     5041 gccaaacgca gcaaaatggg cggtgtgcga gccgccgccg agatcctgaa cttcatgaac
     5101 tcggccgacg aagaagccgt ggtggcgaat ctgcgcgaac aagacgccga cctgacacaa
     5161 cgcatcgtcg acgaaatgtt cgtgtttgac aacctggccg acatcgagga caagtccata
     5221 caaatgatcc tgaaggaagt cgacaccacg tcgttgacca tcgccctcaa gggcgcctcc
     5281 gaggatctgc gggagaaatt cttccgcaac atgtcggccc gggccgccga gatcctgcga
     5341 gacgacctgg atgcccaagg ccctgtacgc atgtcacgtg tcgagcagga gcagaaagcc
     5401 attctgcaga tcgcccgcaa gttggccgaa gcgggacaaa tcacgctgga caacccgggt
     5461 aacgacgaat atgtctgacg aaaccgccct tgacgtcgct gccgcacgcg cccgctggcg
     5521 gcgctggaaa atgtcgggct tcgacaccgc tgcgaacgcc atcgtggacg gacaagcacc
     5581 ggcgcaccgc gccgaggaac tcgccgcacg cgttcagcaa gcacgcctgg ctgcatacga
     5641 acaggcccgc gtggagggtc atggactggg ccatgccgaa ggattgcgga gcggcctgga
     5701 cgaaggccgc gcggcgggcc gtgaagaagg ctatcaggcc ggctatgaag caggacatgc
     5761 cgacggtcgc gcccaaggcg cggagcaagc cgcagccgaa gccacgcgcc tggcggggct
     5821 ggcgggagct tgcgcgcagt cgctcgcagc gctggagaat gaagtcggcg cggcgctcgc
     5881 gtcgttgagt ctgcgcatcg ccgagcacat cgtgcgcggt gcgctggaca tcgaaccgga
     5941 gcgtatcgtc gatctggtcc gccaggtgct gggcaaccga gacaccgacc aggacgtgtc
     6001 cctgactcta cacgtgcatc ccagcgatct cgaactcgtg cgccactata tgaagaacga
     6061 ccaccccggg gacaaatggc gcttgtgcgc ggtggagtcg atgacgcccg gcggctgcct
     6121 catccactcg tccctgggcg cggtggacgc cacgctggaa acccgctggc acaacgtttc
     6181 cgccgcgctc ggcgtcctcc cgcctcccgg acagtcccct cgtggaacct gaccacaccg
     6241 acacgcccgg cgcgcgctgg cggctgcggc tggcggccga cgccagacgc attccgctcg
     6301 tgccgccatt caccgccagc ggcagagtga cacgcgccac tggcttggtg atcgaggcca
     6361 cagggctgcg aatggccgtc ggcgccgcat gccgaataga aatcgcaccc ggaagcgagc
     6421 gctgggcgga cgcggaggtg gtgggcttcc atggccaggt cttgtatttg atgccccaga
     6481 gcgacatctc ggggctgcca cccggcgcgc gcgtgctgcc cgccgaaccc gacaaacccg
     6541 ttccgggaaa cgccacgaca caagaccggc gggccatgtc ggcacaaacc cggcgccttc
     6601 cggttgggga aaccctgctt ggccgtgtcg tggacggctc gggcaatccg ctcgatggac
     6661 ttggccccct gcgccacgcc catatggcgc ccctgacggc acgggcaatc aacccgcttg
     6721 cgcgagcgcc catcgacacg gtgctcgacg tgggcgtgcg cgccgtcaac gggctgctaa
     6781 ccgtcggccg aggccaacgc atggggctgt tcgccggttc cggggtcggc aaaagcgttc
     6841 tgctgggcat gatggcgcgc tacaccgcgg ccgacatcat cgtcgtgggg ctcatcggcg
     6901 agcgcggccg tgaagtcaag gagttcatcg agcacaacct ggggcccgag gggctgcggc
     6961 gctcggtcgt ggtggccgcg ccagccgacg tctcgcccct gctgcgcctg caaggcgccg
     7021 tctatgccac ccgtctcgcc gagtacttcc gcgacatggg gaggcacgtg ctgttgatca
     7081 tggattcact cacccgtttt gccatggcgc agcgcgagat cgctctggcg atcggcgagc
     7141 caccggcgac caaaggctac ccgccatcgg tgttcgccaa gctgccggca ctggtggagc
     7201 gcgccg
//
LOCUS       DASTNK010000004        16945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_744226, whole genome shotgun sequence.
ACCESSION   DASTNK010000004 DASTNK010000000
VERSION     DASTNK010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 16945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16945
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744226"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VFR20_00280"
     CDS             <1..312
                     /locus_tag="VFR20_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0228981.1"
                     /translation="FDTWDVLRTYNDDWRLIIVGDATMSPYEILQVGGSVEHYNTEPG
                     AAWLQRLLEAWPRSAWLNPEPQGSWHYRQSIALVKDMMHDHMYSVTVDGLEQAMRQLS
                     K"
     gene            392..3256
                     /locus_tag="VFR20_00285"
     CDS             392..3256
                     /locus_tag="VFR20_00285"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU0228982.1"
                     /translation="MPIFFHRLARRAGELLALALVTAPAWALTLPAGVTRGPSVQGVT
                     QYTLTNGLRVVLANDDSQSTTTVNMTYLAGADRENYGQAGMAHVLEHMMFRGTPRLRD
                     PMAAFSRRGLAANGSTTNDRTSFYATFAADPKTLDWFLDWQADEMVHALLSPEDLTTE
                     RKVVLSEMERDNNSPVQVLLQTMRSTAYQWNHYGHTAMGTRSDVESVNVGELRTFYHK
                     YYQPDDAVLVITGRFDPAQTLRVIATAFGKIPRPTRPLLPDHTVEPVQDGARQVTLRR
                     HGGSPYVGALYHIPQAASPQYIPVSLGVAILTDTPSGRLYHALVKTRLSTSIDGFAAA
                     MRQPGYAFFGAQLAPTIDPDKALAAMEKVLDSIGSRPFTQEELGRIKREWLTGWDQTY
                     ADPGALAAALASASAHGDWRLFFLDRDRVKNATLAQVQDATQAYLVGSNRTTGQYLPT
                     IDPVRAPASTPVDLAALFKHFQGGKAPVAVPAFDASPANIDARTQRHSLHLPNGTVKL
                     ALLSKPTRGDTVQALMLLRFGNVEDLRGTRSVSRAVANLLDNGTTDMTRQQIQDRFNQ
                     LQANVDFSGSGGAVVVNLSCKGEYLPQVTALVLKILRQADFPESELANYQRQAAASIE
                     DAMSSPNARAAQALARHDNPWPKDDIRYTPSFDETLHAVQSLTRQDLLDFHNKFYGTG
                     NIEFSAVGQFDTKALEQALTAGLKGWHKAPAHQRVPNPYRAVPPQTFTLNTPGKTNAV
                     YLSTLPVKVKDSDADYPALFLANYLLGGSETSRLWNRLRVQDGLSYTVQSSLDVSSHE
                     PSGQWSIYAITAPKNTSHVQSDVAAVLAEVLSKGFTPEEVKQGIASILNYRKLQLTRD
                     GAVASTWIDYMRLGRDYTWWSDLDKKLAGLTASKVNAALRAVLRPSQFSTAIAEDVAA
                     AKQPQAVPAGKKPETKSPGESQGPGPQKRSAPLSGEQGVN"
     gene            complement(3249..4367)
                     /gene="dnaJ"
                     /locus_tag="VFR20_00290"
     CDS             complement(3249..4367)
                     /gene="dnaJ"
                     /locus_tag="VFR20_00290"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU0228983.1"
                     /translation="MAQRDLYEILGVAKNASDDELKKAYRKLAMKYHPDRNPGNKEAE
                     EKFKEAKQAYEILADKEKRGAYDRFGHAGVDPSNAGMGGMGGAGFADAFGDIFGEIFG
                     GGRRSGGPQVYRGADLKYTLEISLEQAAAGFDTEIRVPSWETCDVCHGSGAKPGTQPR
                     TCHSCGGSGAVRMQQGFFSVQQTCPTCHGTGKEITDPCQACEGVGRTRKNKTLQVKIP
                     AGIDDGMRIRSSGNGEPGVNGGPPGDLYVEIHLKPHGIFQRDGEDLHCELTIPFTKAA
                     LGGPLEVPTLTGKGEISIPEGTQTGKTFRLRGKGIRGVRSSYPGDLYCHVSVETPVRL
                     SDEQKKLLRDFEISLSQGGDRHSPKSESWTDKVKSFFS"
     gene            complement(4513..6447)
                     /gene="dnaK"
                     /locus_tag="VFR20_00295"
     CDS             complement(4513..6447)
                     /gene="dnaK"
                     /locus_tag="VFR20_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU0228984.1"
                     /translation="MSKIIGIDLGTTNSCVAVLEGGNVKIIENSEGARTTPSIVAYMQ
                     DGEVLVGTPAKRQAVTNPKNTLYAVKRLIGRKFEEKAVQKDLHLMPYRIFKADNGDAW
                     VEAQGKKLAPPQISADVLRKMKKTAEDYLGEEVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAFGMDKADTRDRKIAVYDLGGGTFDISIIEIADVDGEKQFE
                     VLSTNGDTFLGGEDFDQRIIDYIIAEFKKESGVDLSKDVLALQRLKESAEKAKIELSS
                     TQQTEINLPYITADASGPKHLNLKITRAKLEALVEDLIERTIDPCRTAITDAGVNVSE
                     IDDVILVGGMTRMPKVQEKVKEFFGKDPRKDVNPDEAVAAGAAIQGSVLAGDRKDVLL
                     LDVTPLSLGIETLGGVMTKMIQKNTTIPTKFSQVFSTADDNQPAVTIKVYQGEREIAA
                     GNKALGEFNLEGIPPASRGTPQIEVTFDIDANGILHVSAKDKGTGKENKITIKANSGL
                     SEDEIQRMVKDAEANAEDDHRIAELAQARNQGDALVHSTRKSLEEYGDKLDAADKERI
                     EAAIKDLEEALKDGDKATLDAKVEALSTASQKLGEKMYADMQAQAATGQQGGAEAGAG
                     AAPADENVVDADFKEVKREH"
     gene            complement(6580..7176)
                     /gene="grpE"
                     /locus_tag="VFR20_00300"
     CDS             complement(6580..7176)
                     /gene="grpE"
                     /locus_tag="VFR20_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU0228985.1"
                     /translation="MSSPNEPLDPARRDQPVDTSEKLEVNEKQGGEAGAAGLESAPAE
                     DLASLLAEAQTQVAQYHDQLLRAKAEVENIRRRSQEEVAKARKFGTESFAESLVPVKD
                     SLEAALSQAEQTADSWKEGVEATLKQLNVAFERNLLREVAPAPGDKFDPHAHQAISSI
                     PSDLPEGAVVDMLQKGYTIADRVLRPALVTVSSGQPAG"
     gene            complement(7549..7706)
                     /locus_tag="VFR20_00305"
                     /pseudo
     CDS             complement(7549..7706)
                     /locus_tag="VFR20_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015061.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
     gene            complement(7703..8713)
                     /gene="hrcA"
                     /locus_tag="VFR20_00310"
     CDS             complement(7703..8713)
                     /gene="hrcA"
                     /locus_tag="VFR20_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU0228986.1"
                     /translation="MDDRANALLKALIERYIADGQPVGSRTLSKMFDLSPATIRNVMA
                     DLEELGLIHSPHTSAGRVPTPRGYRMFVDRLLAVQQFEALPASQVRQMLPAAEPTRAI
                     NAAAALLSNLSQFAGVVLTPRRTQVFRHIEFIRLSDKRVLLIIVTPDGDVQNRILFAQ
                     RDYQDQELIEAGNFFNQHFSGMSFEQVRGTMASELVSLQQDISRLMQAAVEAGTAPVD
                     SDETVVISGESKLLDIFEIASDMDRLRGMFALFEKKTDLLQLLDVSSRAQGVQIYIGG
                     DSQLVPLEDVSVITAPYGVDGQVVGTLGVIGPSRMAYERVIPIVDITARLLSNALSNN
                     PS"
     gene            8871..9770
                     /locus_tag="VFR20_00315"
     CDS             8871..9770
                     /locus_tag="VFR20_00315"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU0228987.1"
                     /translation="MHFKTVAFVGRHQDHGLDGPLRTLAAHLRRAGCDVLVETDTASN
                     TRITEYPVCGYDEIGARADLAVIMGGDGTMLGAGRRLAGTRVPLIGINHGRLGFITDI
                     PLQNAVEALTSVINGNFEAEDRVLLEGRVVRGDQTLYSGVALNDVVINRAGRGGMIEL
                     RVELDGTFMYSQRADGLIMATPTGSTAYSLSANGPILHPSVKAIVLVPVAPQTLSNRP
                     IVIPESGTLSLTITALGRVDSGASVHFDMQTWSDCQPGDRIDVRCAPHTVRFIHPSGY
                     SFFSTLRRKLHWNHMPRPTDEED"
     gene            9787..11433
                     /gene="recN"
                     /locus_tag="VFR20_00320"
     CDS             9787..11433
                     /gene="recN"
                     /locus_tag="VFR20_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU0228988.1"
                     /translation="MLRTLHIRDFVIVDQADIPFEPGFTVFSGETGAGKSILIDALSL
                     ALGARTDPGVVREGRPRADVSAVFDAPDTLHGWLRERELDGEDDTLILRRVVDTQARS
                     RAFVNGLPVTLAQLRELAEHLVDIHGQHAHQSLLKSSSQRNLLDDQGGHQALASEVRQ
                     AWHVWQQADKALLAATQNAGRMQQERERLAWQTAELDRLDLHEGEWEKVSNDHSRLAH
                     AQALQDGARQALAALDGDDTSAQQFLGAAEHHLKNLVRHDAHLQGILDTLASARIAAT
                     DAASDLGGYLDRLELDPDSLARAEQRMSAIFETARKFRVEPDELPALRQSLHDQSQAA
                     QSAHDIPALTERVAAARNTYDAAAVRLSDARKKAGKTLAKQVTKAMQTLAMRGGRFDI
                     SLEPCEPGAHGSEQVEFLVAAHEGVTPRPLAKVASGGELARLSLALSVIASQAARVPT
                     LIFDEVDAGVGGAVAEVVGRLLRELGERHQVLCVTHLPQVAARGAHHYEVRKATSDGS
                     TLSMIQALNQQGRVDELARMLGGLTITDTTRRHAREMLTE"
     gene            complement(11526..11960)
                     /gene="fur"
                     /locus_tag="VFR20_00325"
     CDS             complement(11526..11960)
                     /gene="fur"
                     /locus_tag="VFR20_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEU0228989.1"
                     /translation="MNEQNELKTMGLKATFPRLKILDVFRKADHRHQSAEDVYRALIN
                     EEVEIGLATVYRVLTQFEQAGILVRSQFDGGKAVFELNDGDHHDHLICTNCGKVVEFT
                     DTDIEKRQHKVAKENGFTLESHTMMLYGICPDCAKLPHHGGR"
     gene            12137..12781
                     /locus_tag="VFR20_00330"
     CDS             12137..12781
                     /locus_tag="VFR20_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEU0228990.1"
                     /translation="MRVALVAGATALTLSACGTTNWGFPYKMDVQQGNWITSEQVAQL
                     RKGMTPDQVRFILGSPTLHDVLHADRWDYPYLNEPGYGKREDRNFTVWFKNGLLDHWK
                     GDAQPDRQPFQKTDTGAKAIEKSGSGAGAPGSGTAGASSENAKTGATGTESGQAAPVN
                     SSSYHAPTNTLKPGIAPANNTTDNHVSRQSDLLLKAPAPITDPSAGGATSQPLR"
     gene            12792..13583
                     /gene="dapB"
                     /locus_tag="VFR20_00335"
     CDS             12792..13583
                     /gene="dapB"
                     /locus_tag="VFR20_00335"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU0228991.1"
                     /translation="MRIAIAGANGRMGRMLIEAVLASPDLTLTVALAPQGATCLGADA
                     GAFLGRDSGVRITDDLSALAQADCLIDFTRPAGTLNHLLACQEHGTQCVIGTTGFDEQ
                     GKQAIREASKKIAIVFSANMSVGVNVTLKLLNMAAHLLNGGYDVEIFEAHHRNKVDAP
                     SGTALAMGEAIAGAWDKKLEDIADWARHGETGARETGRIGFSSLRGGDIVGDHTVFFC
                     GTGERIEISHRSSSRATYAQGSLRAARYLAGKESGLFDMQDVLQT"
     gene            complement(13707..14738)
                     /gene="murB"
                     /locus_tag="VFR20_00340"
     CDS             complement(13707..14738)
                     /gene="murB"
                     /locus_tag="VFR20_00340"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU0228992.1"
                     /translation="MTVNGSAAQRFNQDLTAFNTFGLPSRASALWRYSSMSELAGLSN
                     VAKAHDRVFVLGGGSNVVLAPHLDSLVVKVESRGVRVHDESPDDWVIEAQAGEVWHDF
                     VAHCIANGWHGLENLALIPGTVGAAPVQNIGAYGVELKDRFHSLQAWDMRGGCLVDMT
                     PTDCRFSYRDSVFKQAPPGRWLIVSVRFRLPKQWRPVLDYPDLKRYFGESPLQVAPRQ
                     VFDAVSDIRRRKLPDPSRLGNVGSFFKNPVVSADRFHALKTRWPDLVAYPQENDTYKL
                     AAGWLVERAGWKGRRLGSVGVHERQALVLVNYGGADAEAVADLANRVRGDVSARFGVE
                     LEQEPVAVH"
     gene            15056..15131
                     /locus_tag="VFR20_00345"
     tRNA            15056..15131
                     /locus_tag="VFR20_00345"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15089..15091,aa:Gly,seq:gcc)
     gene            15299..15550
                     /locus_tag="VFR20_00350"
     CDS             15299..15550
                     /locus_tag="VFR20_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEU0228993.1"
                     /translation="MQTFTANEAKTRFGELIDRVQREPIQVTRRNRVVGVMVSAEDYH
                     AMREFYADRLRRTLRQSAEEAVAKGLTDEKLQQILADED"
     gene            15540..15968
                     /locus_tag="VFR20_00355"
     CDS             15540..15968
                     /locus_tag="VFR20_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HEU0228994.1"
                     /translation="MRIERAVIDTNVLISAALSPRSAPAQIVDCLLRHATLVFSRETF
                     EELETRLWRPKFDRYLDIDRRRLLLHDFAAVAQWVDLPVSPRPTYSRDPDDDVFLHTA
                     LLGKAEWLVSGDRDLLELAAGDWGFEILSPAQALKKWHQK"
     gene            16245..16913
                     /locus_tag="VFR20_00360"
     CDS             16245..16913
                     /locus_tag="VFR20_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEU0228995.1"
                     /translation="MTPLPVPRSRISHSLALLVAFHVLVIISSNYLVQLPIDLFGFHS
                     TWGAFSFPFIFLATDLTVRLIGKTEARIVIAKAMVPALIASYVVSVLFHAGRFNGFGA
                     LGDFNGFVFRIALASFTAYVLGQLLDVQVFDRMRRSHMQWWLAPAASSALGQALDTAA
                     FFSVAFWHSDNPFMTEHWPEIALVDYVIKLTVSLLLFLPLYGIALNAIIRFMKRHEIR
                     LAGA"
BASE COUNT         3191 a         5644 c         4998 g         3112 t
ORIGIN      
        1 ttcgacacct gggacgtcct gcgcacctat aacgatgact ggcgcctgat catcgtgggc
       61 gacgccacca tgagccccta cgagatcctg caggtgggcg gctcggtgga gcactacaac
      121 accgagcccg gcgccgcctg gctgcagcgc ctgcttgaag cctggccccg ttcggcctgg
      181 ctgaaccccg aaccccaagg gtcctggcat tatcgccagt ccatcgccct ggtcaaggac
      241 atgatgcatg accacatgta ttctgtgacc gtggatggcc tggaacaagc catgcgccag
      301 ttgtcaaaat gaacccacgg gcaagatcgg cgtcaaacca cccagccgcg ccttccagcg
      361 cacttacccg caacgtccac ccgaggcttt catgccgata tttttccatc gcctcgcccg
      421 ccgcgcgggc gaactgctcg cgcttgccct ggtcaccgcc cccgcctggg cgctcacgct
      481 cccggcgggc gtcacgcgcg ggccatccgt gcagggcgtc acgcaataca cgctgaccaa
      541 tgggctgcgc gtggtgctgg cgaacgatga ctcccagtcg acgaccaccg tgaacatgac
      601 ctatctggcc ggcgccgacc gcgagaacta tggccaggcc ggcatggcgc acgtgctgga
      661 gcacatgatg tttcgcggca cacccaggct gcgcgacccc atggccgcct tctcgcggcg
      721 cggcctggcc gccaatggct cgacgaccaa tgatcgaacg agcttctatg caaccttcgc
      781 cgccgacccc aagacgctgg attggttcct ggactggcag gcggacgaaa tggttcatgc
      841 cctgctgtcg ccggaggacc tcacgaccga gcgcaaggtg gtgctcagcg aaatggagcg
      901 tgacaacaac agtccggtcc aggtgctgct gcaaaccatg cgttcaactg cgtaccagtg
      961 gaaccattac ggccacaccg cgatgggtac gcgctcggac gttgagagcg tcaatgtggg
     1021 cgaactgcgg acgttttatc acaagtatta ccaacccgac gatgccgtcc tggtcatcac
     1081 gggccggttc gatccggccc agacgctgcg cgtcatcgcg acggccttcg gcaagatacc
     1141 caggccaact cggccgctgc tccccgacca cacggtggaa ccggtgcagg atggcgcgcg
     1201 ccaggtcact ctgcgccgtc atggcggcag cccttatgtg ggggcgctgt accacatacc
     1261 ccaggccgcg agcccgcaat acatacccgt gagcctgggc gtcgcaatcc tgacggacac
     1321 cccatcggga cggctgtacc atgccttggt gaaaacacgg ctgagcacgt cgatcgacgg
     1381 tttcgcggcg gcgatgcgcc agccgggcta cgccttcttc ggtgcccagc tcgctcccac
     1441 catcgacccc gacaaggcgc tggccgcgat ggaaaaagtg ctggactcga tcggctccag
     1501 gccgttcacc caggaagaac tcggtcgcat caagcgtgag tggctcacag gctgggacca
     1561 gacctacgcg gaccccggcg ccttggccgc ggccctggcc agtgcaagcg cccatggtga
     1621 ctggcgcctg ttcttcctgg accgggaccg cgtcaagaac gccacgctcg cccaggtgca
     1681 ggatgccaca caagcctacc tggtgggtag caatcgcacc acgggccaat accttcccac
     1741 catcgacccc gtgcgggcac ccgcctcgac cccggtcgat ctggcagcgc tgttcaagca
     1801 cttccaggga ggcaaagccc ccgttgccgt gcccgcgttc gacgccagcc ccgccaacat
     1861 cgacgcccga acccaacgcc attcgctaca cctccccaac ggcaccgtga aactggcgct
     1921 gctgtccaag cccacccggg gcgacacggt gcaagcgctc atgctgctgc ggttcggcaa
     1981 tgttgaagac ctgcgcggca cgcgctccgt gtcccgcgcc gtcgccaacc tgctggacaa
     2041 cggcacgacc gacatgacac gccagcagat tcaggatcgc ttcaaccaat tgcaggccaa
     2101 tgtggatttc agcggatccg gtggcgcggt cgtggtgaac ctgtcatgca aaggcgaata
     2161 cctgccgcag gtgaccgccc tggtgctgaa gattctgcgt caggcggact tccctgagag
     2221 cgagttggca aactaccaac gccaggccgc cgcatcgatc gaagacgcca tgtccagccc
     2281 gaacgcgcgc gcggcccagg ccctggcgcg ccacgacaac ccctggccca aggacgacat
     2341 ccgctacact cccagcttcg acgaaaccct gcacgccgtg caaagcctga cacgccagga
     2401 cctgctggat ttccacaata aattctatgg caccgggaac atcgaattct ccgctgtggg
     2461 gcagttcgac acgaaagcac tggaacaagc gctgaccgcc gggctgaaag gctggcacaa
     2521 agctcccgcc caccagcgcg tgccgaatcc gtatcgcgcc gtgccaccac agacgttcac
     2581 cctcaacacc cccgggaaga ccaacgcggt gtacctgtcc acactgcccg tcaaggtgaa
     2641 ggacagcgat gcggattatc ctgcgctgtt ccttgcgaac tacctgttgg gcggctccga
     2701 gacatctagg ctttggaatc gcctgcgcgt gcaggatggg ctgtcctata ccgtgcagag
     2761 cagcctggac gtgtcgtcgc atgagccgtc gggccaatgg tccatctatg cgatcaccgc
     2821 gccaaagaac acgtcacatg tgcaaagcga cgtcgcggcc gtgttggccg aggtgctgag
     2881 caagggcttc accccagagg aagtcaaaca aggcatcgcc tcgatcctga actaccgcaa
     2941 gctccagttg acgcgggatg gcgcggtcgc cagcacgtgg atcgattaca tgcgactggg
     3001 gcgcgactac acctggtggt ccgatctgga caagaaactg gcagggctca cggcatcgaa
     3061 ggtcaacgcg gcgttgcgcg ccgtgctcag gccgtcgcag ttcagcacag ccatcgccga
     3121 ggatgtcgcc gccgcgaagc agccgcaagc cgtgcctgct ggcaagaagc cggaaacgaa
     3181 aagccctggc gaaagccagg gcccgggccc gcaaaagcgc agcgcccccc tcagtgggga
     3241 gcaaggcgtc aactgaagaa gctcttcacc ttgtcggtcc acgattcgct cttgggcgaa
     3301 tgccggtcac caccttgcga tagcgagatt tcgaaatcgc gcagcagttt tttctgctca
     3361 tcggagaggc gcaccggtgt ctcgaccgag acgtggcagt acaggtcgcc tggatagctg
     3421 gaccgcacgc cccgtatgcc tttgccgcgc aagcggaagg tcttcccggt ctgggtgcct
     3481 tcggggatgg agatctcccc tttaccggtg agcgtgggga cttccagcgg accacccagg
     3541 gccgccttgg tgaacggtat ggtcaattcg cagtgcagat cctcgccatc gcgttggaag
     3601 atgccatgtg gcttgaggtg gatttcaaca tagagatcgc ccggcgggcc gccattgacg
     3661 ccgggttcgc cgttgcccga agagcggatg cgcataccgt catcgatgcc ggcgggaatc
     3721 ttgacctgca gcgtcttgtt cttgcgcgtg cgccccacgc cttcgcaagc ctgacaggga
     3781 tcagtgatct ccttgcccgt gccgtggcag gtcgggcagg tttgctggac actgaaaaac
     3841 ccctgctgca tgcgcaccgc gccgctcccg ccacaggaat ggcaggtgcg cggctgggtg
     3901 ccgggcttgg cgcccgaacc atggcagaca tcgcaggttt cccaactggg cacacggatt
     3961 tcggtgtcga acccggcggc ggcctgctcc aggctgatct cgagcgtgta cttcaggtcg
     4021 gcgccgcggt agacctgcgg gccgccgctg cggcgcccgc ctccgaatat ctcgccgaag
     4081 atgtcgccga aggcgtcggc gaaacccgca ccgcccatgc cgcccatgcc ggcgttgctg
     4141 gggtccaccc ccgcgtggcc gaaccggtcg tacgccccgc gcttctcttt gtcggcgagg
     4201 atctcgtagg cctgcttggc ctccttgaac ttttcctcgg cctctttatt gcccggattg
     4261 cgatccgggt ggtacttcat ggccagcttc cggtaagcct ttttcagttc gtcgtcggac
     4321 gcgttcttcg ccacgcccag aatttcatac aaatcgcgtt gtgccatagt gtcgccggcg
     4381 ttgctaacgc ccgttcctca cttcagaata agaacgagaa cgcccggttt tcgacgagac
     4441 cgccgaaaac cgggcttggc cgggccgcgc ggccaagggc caccgcagcc ttccgctcat
     4501 gcggtccggc cgtcaatgct cacgcttgac ttccttgaag tcggcgtcaa ccacgttctc
     4561 gtcggcgggc gccgcgcccg cccccgcttc ggcgccgccc tgttgacccg ttgccgcctg
     4621 ggcctgcatg tcggcgtaca ttttctcgcc cagcttctgc gaggcggtcg acagcgcttc
     4681 gaccttggcg tcgagcgtcg ccttgtcacc atctttgagc gcctcttcga ggtctttgat
     4741 ggcggcctcg atgcgttcct tgtcggcggc gtcgagcttg tcaccgtatt cctcgagcga
     4801 tttgcgggtg gaatgcacca gcgcgtcgcc ctggttgcgc gcctgggcca gctcggcgat
     4861 gcggtgatca tcttcggcat tggcctcggc gtctttcacc atacgctgga tctcgtcctc
     4921 gctcaggccg gagttcgcct tgatggtgat cttgttctcc ttgcccgtgc ccttgtcctt
     4981 ggccgagacg tgcaggatcc cgttggcgtc gatgtcgaag gtgacctcga tctgcggggt
     5041 gccgcgcgac gcgggcggaa tgccttcaag gttgaattcg cccagcgcct tgttcccggc
     5101 cgcgatttcg cgctcaccct ggtacacctt gatggtgacc gcgggctggt tgtcgtcggc
     5161 cgtggagaac acctgcgaga acttcgtggg aatggtggtg ttcttctgga tcatcttggt
     5221 catgacaccg cccagggttt cgatccccag cgacagcggc gtcacgtcga gcaagagcac
     5281 gtccttgcga tcaccggcca gcaccgaacc ctggatggcc gcgccggcgg ccacggcctc
     5341 gtcagggttg acatccttgc gcggatcctt gccgaagaat tctttcactt tttcctgcac
     5401 cttgggcatg cgggtcatgc cgccgaccag gatcacgtcg tcgatctcgg acacattgac
     5461 gccggcatcg gtaatggcgg tgcggcaagg atcgatggtg cgttcgatca ggtcctcgac
     5521 cagggcttcg agcttggcgc gcgtgatctt caggttcagg tgcttgggac cggaggcatc
     5581 ggccgtgatg tacggcagat tgatctcggt ctgttgcgtc gacgagagtt cgatcttggc
     5641 cttttccgcc gattccttca ggcgttgcag ggcgaggacg tccttcgaca ggtcgacgcc
     5701 gctttccttc ttgaattcgg cgatgatgta atcgatgatg cgctggtcga agtcttcgcc
     5761 acccaggaac gtgtcgccat tggtggacaa cacctcgaac tgcttctcgc catcgacatc
     5821 ggcgatctcg atgatcgaga tgtcgaacgt gccgccgccc aggtcataca ccgcgatctt
     5881 gcggtcacgg gtgtcggcct tgtccatgcc gaaggccaac gccgccgccg tgggttcgtt
     5941 gatgatgcgc ttgacctcga gccccgcgat gcggcccgcg tctttcgtgg cctggcgctg
     6001 gctgtcattg aagtatgccg gcacggtgat cacggcctcg gtcacttctt cgccgaggta
     6061 gtcttccgct gtcttcttca tcttgcgcag cacatcggcc gaaatctgcg ggggcgccag
     6121 cttcttgccc tgggcctcga cccatgcgtc gccgttgtcg gccttgaaga tgcgatacgg
     6181 catcaggtgc agatctttct gaaccgcctt ttcctcgaac ttgcggccga tcagccgctt
     6241 gacggcatac agcgtgttct tgggattggt caccgcctgg cgcttggccg gcgtgccaac
     6301 caacacttcg ccgtcttgca tgtaggcgac gatggacggc gtggtgcgcg cgccttccga
     6361 attttcgatg atcttgacat tgcccccttc aagcacggca acacaactgt tggtcgtgcc
     6421 gaggtcaata ccgatgattt tgctcatgat ctgcaatcct gaaaataatt gctgaaaaac
     6481 agtggaaatg aatctggtta tgtcctagct gggggccgaa accgtgaatt tcaagactcg
     6541 gacgtgccgc ccccgccagc cgcccgttgg cacggcggct caaccggcag gctgacccga
     6601 cgagaccgtg accagggcgg gcctcagcac gcggtcggca atggtatagc ctttctgcag
     6661 catgtccacg accgcccctt cgggcagatc ggacggaatc gacgagatgg cctgatgcgc
     6721 gtgcgggtcg aacttgtcac ccggcgcggg ggccacctcg cgcagcaggt tgcgttcgaa
     6781 cgccacattc aattgcttca gcgtggcttc caccccttcc ttccaggagt cggcggtttg
     6841 ctcggcctgg gagagggccg cctcgaggct gtccttgacc ggcaccaggc tctcggcgaa
     6901 cgattcggtc ccgaatttgc gcgctttggc gacttcttcc tgcgaacgtc ggcgaatgtt
     6961 ctcgacctcg gccttggcgc gcagcagttg gtcatggtac tgcgcgacct gggtctgggc
     7021 ttcggcgagc agcgaggcaa gatcctcggc gggcgcggat tccagccccg cggcgccggc
     7081 ctcgcccccc tgcttttcgt tgacttcgag tttttccgac gtatctaccg gttgatcccg
     7141 tcgcgcggga tcaaggggtt cgttcggtga cgacataaat ctctccacat ctcaaaaaac
     7201 gttcaattca gtcaaaatgg ggacgattac cctcgtttca aggccttgaa cgcgaaaaac
     7261 aatgactggc cgtttggaca acacgcattg gcccgccgcg cctcgaccga cccagcacag
     7321 tatttgacgc tatcagagac atgaccgcgt cttttcggcc tgggcgcagc accaaagaag
     7381 gtcgatgcat cccatctggc tccatgagtg tgacaaattt gttcacacac ccaaggcggc
     7441 cagccattca ccggcccggc cagcgccgga aaagtccgcc ccccccggaa atgtggctca
     7501 gccgatcggg acggaaaccc agccggacag gtggcacgtc aggaggccgg ccaggaattc
     7561 cgccaaagac cggcgaatcg cgcctggcgg ggttcatcgg gtgtgccaag gttgatcagc
     7621 agcacgccca cggcacctat ttcccgcgcg ggcgggtcgg gatcgaagcg gtgcggatcg
     7681 ggtgcgcccg gcagaaagcg cgtcatgacg ggttgttgct gagcgcgttc gacagcagcc
     7741 gggctgtgat gtccacaatg gggatgaccc gttcatacgc catgcgcgac ggcccgatca
     7801 cccccagtgt gcccaccacc tggccgtcca caccgtaggg tgccgtgatc accgacacgt
     7861 cttccagcgg caccagttgc gaatcgccac cgatgtatat ctgaacgcct tgggcgcggc
     7921 tggacacgtc cagcaattgc agcagatcgg tctttttctc gaacagggcg aacatgccgc
     7981 gcaggcggtc catgtcggag gcgatctcga agatgtccag cagcttgctt tcaccggaaa
     8041 tgaccacggt ttcgtctgaa tcgaccggcg cggtgcccgc ctcgaccgcg gcctgcatca
     8101 gacgcgaaat atcctgctgc agcgaaacca gctcgctcgc catggtgccg cgcacctgct
     8161 cgaacgacat gcccgagaaa tgctgattga agaaattgcc cgcctcgatc aactcctggt
     8221 cttgatagtc gcgctgggcg aacaggatgc ggttctgcac atcgccatcg ggtgtgacga
     8281 tgatcagcaa cacccgcttg tccgacaggc ggatgaattc tatatggcga aagacctggg
     8341 tgcgccgcgg cgtgagcacc acgccggcga actgggacag attggacagc agcgccgcgg
     8401 ccgcgttgat ggcccgggtc ggctcggccg cgggcagcat ttgtcgcacc tggctggcgg
     8461 gcaaggcctc gaactgctgc acggcgagga ggcgatcgac gaacatgcga tagccgcgcg
     8521 gcgtgggtac ccgaccggcc gacgtatggg ggctgtggat caagccgagc tcttccagat
     8581 cggccatgac attgcgtatg gtggcgggag agaggtcgaa catcttcgac agcgtgcgcg
     8641 accccacggg ctggccatca gctatgtagc gctcgatcag cgccttgagg agggcattcg
     8701 ctctatcatc catacgtcta ttttatgcaa ttcccaccca cgcgcccgac attccgccgc
     8761 tgggggcgga aaccgggccc gcggccgctg gtttccacga ctgccgcaag ccgtggcggc
     8821 cacttataat caatgccaca tccacatttt atccgcgagg cccgcgcccc atgcacttca
     8881 aaacggtcgc gttcgtcggc aggcaccagg accacggcct cgatggcccg ttacgcacac
     8941 tcgcggcgca tctgcgtcgc gcaggttgcg acgtcctcgt cgaaaccgac accgccagca
     9001 atacccgcat cacggaatac ccggtatgcg ggtacgacga aatcggcgcg cgcgccgatc
     9061 tggccgtcat catgggtggc gacggcacca tgctgggcgc cggcaggcgt ctggcaggca
     9121 cacgcgttcc cctcatcggc atcaaccatg ggcgtctcgg cttcatcacc gacatccccc
     9181 tgcagaacgc cgtcgaagcc ctcaccagtg tcataaacgg caatttcgaa gccgaagacc
     9241 gcgtcctgct ggaaggccgc gtcgtgcgcg gcgaccaaac cctgtattcg ggcgtggccc
     9301 tcaacgacgt cgtcatcaac cgcgcgggcc gcggcggcat gatcgaattg cgggtcgagc
     9361 tcgatggcac gttcatgtac agccagcgcg ccgacggcct catcatggcc acgcccaccg
     9421 gttccacggc gtattcgctg tcggccaacg gccccatcct gcaccccagc gtcaaggcca
     9481 tcgtcctggt gcccgtggcc ccgcaaaccc tgtcgaaccg tcccatcgtc atccctgaaa
     9541 gcggcacgct cagcctgacc atcaccgcgc tcgggcgggt agactccggc gccagcgtgc
     9601 acttcgacat gcagacctgg tccgactgcc agcccggcga ccgcatcgac gtgcgttgcg
     9661 cgccacacac cgtgcgcttc atccacccct ccggctacag ctttttctcg acactgcgtc
     9721 gcaagctgca ctggaaccac atgccccgtc ccaccgacga ggaagactag agcgaacaag
     9781 cccccgatgc tgcgcaccct gcatatccgc gacttcgtca tcgtcgacca ggccgacatc
     9841 ccattcgagc ccggcttcac ggtcttttcg ggtgaaaccg gcgccggcaa gtccatcttg
     9901 atcgacgccc tgtcattggc gctgggcgcg cgaaccgacc cgggtgtcgt ccgcgaaggc
     9961 cgcccgcgcg ccgatgtcag cgcggtcttc gacgcccccg acacactcca tggctggttg
    10021 cgcgagcgcg aactcgatgg cgaggacgac acgctcattc ttcgacgcgt ggtcgacacc
    10081 caggcgcgca gccgcgcctt cgtcaacggc ctgcccgtca cgctggccca gttgcgcgaa
    10141 ctggctgaac acctggtcga catccacggc caacacgccc accagagcct gctcaaatcg
    10201 tccagccagc gcaacctgct cgacgaccag ggcgggcacc aagccctggc cagcgaagtc
    10261 cgccaggcgt ggcacgtctg gcagcaagcc gacaaggccc tgctcgccgc cacacaaaac
    10321 gcggggcgga tgcagcaaga gcgtgagcgg ctggcgtggc aaacggccga actcgatcgc
    10381 ctcgacctgc acgagggcga atgggaaaag gtgtccaacg accacagccg cctggcgcac
    10441 gcacaggcac tgcaagatgg cgcccgccag gccctggccg cgctcgatgg cgacgacacc
    10501 tcggcccagc agttcctcgg cgcggccgaa catcatctca agaatctggt gcgccacgac
    10561 gcccacctgc aaggcattct ggacaccttg gcgtcggccc gcatcgccgc caccgacgcc
    10621 gcgtccgacc tcggcggcta tctcgaccgc ctcgaactcg accccgacag tctggcgcgg
    10681 gccgagcagc gcatgtcggc catcttcgag accgcgcgca agttccgggt cgagcccgat
    10741 gagctgccgg ccttgcgcca atcactgcat gaccagtcgc aggccgccca aagcgcccac
    10801 gatattcccg cgctcaccga gcgtgtcgcc gccgcccgca atacctatga cgcagccgcc
    10861 gttcggctgt ccgatgcccg caaaaaagcc ggcaagacgc tggcgaaaca ggtcaccaag
    10921 gccatgcaga ccctcgccat gcggggcggg cgtttcgaca tctccctgga accctgtgaa
    10981 cctggcgcac acggcagcga acaggtggaa ttcctggtcg ccgcacacga gggcgttaca
    11041 ccgcgtccct tggcaaaggt cgcgtcgggc ggcgaattgg cgcgcctgtc tctggcgctg
    11101 tcagtcatcg ccagccaggc cgcccgggtg cccaccctca ttttcgacga ggtcgacgcc
    11161 ggggtcggtg gcgccgtggc cgaggtcgtg ggacgcctgt taagagaatt gggcgaacgc
    11221 caccaggtgc tgtgcgtgac gcacctgccg caagtggctg cgcgtggcgc gcaccattac
    11281 gaagtgcgca aggccacgtc cgacggcagc acgttatcga tgatccaggc gctgaaccag
    11341 caaggccgcg tcgacgaact cgcccgcatg ctgggcggcc tgaccatcac cgacaccacg
    11401 cgccgacatg cccgcgaaat gctgactgaa tgaatccctg cagccgcaag ccgcagatgc
    11461 atgcggcacg gtggcttgtt caacggatcc gcagaaggcc agttaccccg cccccatttg
    11521 gacgatcaac gcccgccatg atgcggcagc ttggcgcagt cggggcagat gccgtagagc
    11581 atcatggtgt ggctctccag tgtgaagccg ttttccttgg ccaccttatg ctggcgtttc
    11641 tcgatgtcgg tgtcggtgaa ttccaccacc ttgccacagt tggtgcaaat aaggtggtcg
    11701 tggtgatcgc cgtcgttcag ttcgaacaca gccttgccgc catcgaactg gctgcgcacc
    11761 aggatgcccg cctgctcgaa ctgcgtcagc acgcgataca ccgtggccag accgatctcg
    11821 acctcttcgt tgatcagggc gcgatagacg tcctcggcgc tctggtggcg gtggtcggcc
    11881 ttgcggaaaa catcgagaat cttcaggcgc ggaaaggtcg ccttcaaccc catggttttc
    11941 aactcgttct gttcgttcat agctaaaatg tctctggctg gaagtaagtg gcatcttcac
    12001 gcctggcccg aaccggatgg cgccgccgca ggaaaggctg atgcgtttat tcttaaacct
    12061 ttatgataac ggttttatcc gctatgaata tcaggggcac tgtgtgctga caaaagcaaa
    12121 atccctacgc tcaaccttgc gtgtggcact ggtggcgggc gccacggcgc tcacgctgtc
    12181 ggcctgtggc acgaccaatt ggggcttccc ctataaaatg gatgtccagc aaggcaactg
    12241 gatcacctcg gaacaggtcg cgcaactgcg caagggcatg acacccgacc aggtccgttt
    12301 catcctgggc tcccccaccc tgcacgatgt gctgcatgcc gaccgctggg actaccccta
    12361 cctgaacgaa cccggatacg gaaagcggga agaccgcaat ttcacggtct ggttcaagaa
    12421 cggcctgctc gaccactgga agggtgatgc ccaacccgac cggcaaccgt tccagaagac
    12481 cgataccggt gccaaggcaa tcgaaaaatc gggctcgggg gccggcgcgc ccgggtctgg
    12541 tacagctggc gcgtcatccg aaaacgcgaa aaccggcgcc acaggcacag aatccggcca
    12601 ggccgctccg gtgaacagca gcagctacca cgccccgacc aacacactca aaccaggcat
    12661 tgcgccggcc aacaacacca cagacaatca cgtgagccgg cagtccgacc tcctgctgaa
    12721 ggcccccgcc cccatcaccg acccctccgc ggggggggcc acaagccagc ccttgcgctg
    12781 acgggggcac catgcgtatt gccatcgccg gagccaacgg ccgcatgggc cgaatgctca
    12841 tcgaagccgt actcgccagc cccgacctga ctctcacggt ggcgctcgcc ccgcagggcg
    12901 ccacctgcct gggcgccgac gccggcgcgt tcctgggccg cgactcgggc gtgcgcatca
    12961 ccgacgatct gagcgccctg gcccaggccg actgcctcat cgatttcaca cggccagcgg
    13021 gtacgctgaa tcacctgctg gcctgccagg aacacggcac ccaatgcgtc atcggcacca
    13081 cgggtttcga tgaacagggc aagcaagcca ttcgcgaagc cagcaagaaa atcgccatcg
    13141 tcttttccgc caacatgagc gttggcgtca acgtgacgct caaactactg aacatggccg
    13201 cacacctgct gaatggcggc tacgatgtgg aaatctttga ggcgcatcac cgcaacaagg
    13261 ttgacgcgcc gtcaggcacc gcgctggcca tgggcgaggc catcgcgggt gcctgggata
    13321 aaaaactgga agatatcgcc gactgggccc gtcacggcga aaccggtgcg cgcgaaacag
    13381 gaaggatcgg gttttcatcg ttgcgtggcg gcgatatcgt cggtgatcac accgtcttct
    13441 tctgcggcac cggcgagcgc atcgaaatca gccaccgctc cagcagccgc gccacctatg
    13501 cccagggcag cctgcgcgcc gcgcgttatc ttgccggcaa agagtccggc ctgttcgaca
    13561 tgcaggacgt gttgcagacc tgatggccag ccgacctgac gacttgccgg cctgatgact
    13621 gccaagccaa cgtgccagcc tcgatgaacc gtcacaacag tgtgcgtgca atgatgtcgt
    13681 ggccggggtc gggccacatg ccagcctcaa tggaccgcca cgggctcctg ttccagctcc
    13741 acgccaaatc gggctgacac atcgcccctg acccggttgg cgagatcagc caccgcttcg
    13801 gcatccgcgc cgccgtaatt gaccagcacc agggcctggc gctcgtgcac acccaccgac
    13861 cccaggcgcc ggcccttcca gcccgcccgt tcgaccagcc aacccgccgc cagcttgtag
    13921 gtgtcgttct cctgtgggta tgccaccaga tccggccaac gcgttttcag ggcatggaaa
    13981 cgatcggccg acaccacggg gttcttgaaa aaactgccga cattgcctaa tcgggaggga
    14041 tcgggcagct tgcgccgccg aatgtcgctg accgcgtcga acacttggcg cggcgcgact
    14101 tgcaatggcg attcgccaaa atagcgtttc aggtcgggat aatcgagcac gggccgccat
    14161 tgcttgggca ggcggaagcg caccgacacg atcagccagc gacccggtgg tgcttgcttg
    14221 aatacactgt cgcgatacga gaaccggcag tcggtgggtg tcatgtcgac gaggcaaccg
    14281 ccgcgcatgt cccatgcctg caggctgtgg aaacgatcct tcagctcaac cccgtacgcg
    14341 ccaatgttct gcaccggcgc cgcgccaacc gttccgggga tcagcgccag attttcaagg
    14401 ccatgccagc cgttcgcgat gcaatgggcc acgaaatcgt gccagacctc gcccgcctgc
    14461 gcctcgatca cccaatcatc gggggattcg tcgtggacac gcacgccccg ggattccacc
    14521 ttcaccacca gactgtccag atgaggcgcc agcacaacgt tgctgccgcc gcccagcaca
    14581 aacacgcggt cgtgcgcctt cgccacgttg gacaggccgg ccaattccga catggacgag
    14641 taccgccaca gcgccgacgc ccgcgaaggc aggccaaacg tattgaacgc cgtcaaatcc
    14701 tggttgaaac gctgcgccgc actaccgttt actgtcattg tccgcacaca aaaaaacacc
    14761 gctcgatcgt cagaatgagg aaacaagacc tcaaaacccg cacaccgcct gcttgtttga
    14821 cttggatttg acaggctagg ccggggtcat gctatgattc cgctccctgc ttgaatgtta
    14881 cttgattttc agtgggcgcg gccaagttgg ttcacccagc cggttgccga agctgaaaac
    14941 ttcatataga atgcagggct tgcttgattc agcctaaact tgcgatacgc agacagtttc
    15001 gcggaaagat tgcgggtata gggttacagc agggttacag caggattgca gcaacgcggg
    15061 aatagctcag ttggtagagc gcaaccttgc caaggttgag gtcgcgagtt cgagactcgt
    15121 ttcccgctcc agattcaaaa aaggcccaaa cttcggtttg ggcctttttt tcgaatggcg
    15181 attctctgac caccttcaca atacaccgga caacctcttc aaacgaccgg atcacaccga
    15241 ttgaacgaat cgaacgattt gagtacactg acctcataga cttgcacgag gcgttgaaat
    15301 gcaaaccttt accgccaatg aggcaaaaac tcgattcggt gaactgatag accgggtgca
    15361 gcgcgagccc atccaagtca cgcggcgcaa ccgtgtcgtc ggcgtcatgg tttccgctga
    15421 agactaccac gcgatgcgtg agttctatgc ggatcggcta cggcgcacct tgaggcaaag
    15481 tgccgaagaa gctgtggcca agggattgac ggacgagaag ctgcagcaga tcctggccga
    15541 tgaggattga acgcgccgtc atcgacacca acgtcttgat aagcgccgcg ctatcgcccc
    15601 ggtccgcgcc agctcagatc gtggattgcc ttctgcggca cgctactctg gtcttttcaa
    15661 gagaaacctt cgaggaactc gaaaccaggc tctggcgtcc gaaattcgac cgctatctgg
    15721 acattgatcg ccgacgcctg ctattgcatg acttcgccgc agtggcccaa tgggtggatc
    15781 tgcctgtgtc gcccaggcca acgtacagcc gcgatcccga tgacgacgtg ttcctccaca
    15841 ccgccttgct tggcaaggct gaatggttgg taagtggcga cagggatttg ctggagctcg
    15901 ctgctggcga ctgggggttc gaaatcctat cgccagctca ggctctgaaa aaatggcatc
    15961 aaaaatgagc aaagcccaat ccacatcgaa gtctaacctg acggaattcc tgcatttctg
    16021 cgatcaagtt gtttttttgg cccaggaagc agggttggtc gtcatgctta gccacgcctt
    16081 atagtcacaa accagcacgc cccgcatcat cgggaaatcg aagccagtat cgaacccccc
    16141 tgtcgccacc tcccgccgcg acatgcaatc gagggttcag cgtacaatcc cctctcgcgt
    16201 ttcctcaatg ggttccctca ccccatccat caaaaaggct catcatgacc ccgctgcccg
    16261 ttccgcgatc gcggatttcc cactccctcg ccctgctggt ggcgttccac gtcctggtca
    16321 tcatctccag caactatctc gtgcagttgc ccatcgatct gttcggcttt cacagcacct
    16381 ggggcgcctt cagctttccc ttcattttcc tagccaccga cctcactgtg cgcctgatcg
    16441 gcaagaccga ggcgcgtatc gtgatcgcca aggccatggt gccggcgctg atcgcgtcct
    16501 acgtcgtctc ggtgctgttc cacgccggcc ggttcaacgg ctttggcgcc ctgggcgact
    16561 tcaacggctt cgtgttccgc atcgccctcg ccagcttcac cgcctacgtg ctgggtcagt
    16621 tgctggatgt gcaggtgttc gaccgcatgc gccgcagcca catgcagtgg tggctggcgc
    16681 cggcggcatc ttccgcgctc ggacaggcgc tggacacggc cgccttcttc agcgtcgcgt
    16741 tctggcacag cgacaacccc ttcatgaccg agcattggcc cgagatcgcg ctcgtcgact
    16801 acgtcatcaa actgactgtc agcctgctgt tgttcctgcc cttgtacggc atcgccctga
    16861 acgccatcat ccggttcatg aaacggcacg agatcaggct ggccggggca tgatcatggc
    16921 cgggaaacac cagcgccgcg ccctg
//
LOCUS       DASTNK010000005         3466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_188273, whole genome shotgun sequence.
ACCESSION   DASTNK010000005 DASTNK010000000
VERSION     DASTNK010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188273"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..263)
                     /gene="rpsP"
                     /locus_tag="VFR20_00365"
     CDS             complement(9..263)
                     /gene="rpsP"
                     /locus_tag="VFR20_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003268924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU0228996.1"
                     /translation="MVVIRLARGGSKKRPFYNLVAADSRNRRDGRFIERVGFYNPVGN
                     EGQENLRISLDRVQYWSDNGAQLSPAVTRLVKEYSAKVAA"
     gene            complement(384..638)
                     /locus_tag="VFR20_00370"
     CDS             complement(384..638)
                     /locus_tag="VFR20_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEU0228997.1"
                     /translation="MMDGNTGDLPAPTLEIDTSGLKCPLPILRAKKALARLESGAVLK
                     VITTDTHAVTDFQAFARQTGNTLLAQVDKGASMEHYLARR"
     gene            complement(759..3383)
                     /gene="alaS"
                     /locus_tag="VFR20_00375"
     CDS             complement(759..3383)
                     /gene="alaS"
                     /locus_tag="VFR20_00375"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU0228998.1"
                     /translation="MKTSEIRQKFLSFFESRGHQIVPSSSLVPANDPTLLFTNSGMVQ
                     FKNVFTGKETRPYSRATSSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     REAITNAWELLTKVYGLPTEKLWVTVYQEDDEAYGIWADEIGVPPERIIRIGDNKGAR
                     YASDNFWQMADTGPCGPCSEIFYDHGPEIWGGPPGSPEEDGDRYIEIWNLVFMQFERD
                     AEGNMPRLPKPCVDTGMGLERIAAVLQHVHSNYDIDLFQNLIRAAARETGTSDLANSS
                     LKVIADHIRACGFLIVDGIIPGNEGRGYVLRRIVRRALRHGHKLGQTKPFFHRLVADL
                     AQEMGAAYPELARLQNRVEQVLRQEEERFSETLENGLKILESALVGVRKGDVLDGDTL
                     FTLYDTYGFPVDLTADICREREVGVDHEGFDHAMAHQRDQARAAGKFKATDELDYHGA
                     ATRFDGYEHLRGTGNITGLYVDGAAVETVSPGQQAIVVLDATPFYAESGGQVGDAGEL
                     LAGGVRFTVSDTQKIQNNVFGHHGVLESGSLKVGDSLDAQVDGARRQRTMRNHSATHL
                     MHKALRQVLGDHVQQRGSLVDPDKTRFDFAHDAPISSDDIARVERIVNEQILANQAVA
                     TRVMPYDQAVEGGAMALFGEKYGDTVRVLDIGFSRELCGGTHVARTGDIGLFKIISEG
                     GVAAGVRRVEAVTGENALAWVQETSGTLAHAADQLKVQPAELGERIAQLQAQLKTAER
                     DLEQARTKLAVQAGNDLADRAAIVLQGDSKLLVGTLTGTDPKILRSMVDQLKNRLKSA
                     IVLLTAEAEGKISLVAGVTPDLSSRVKAGDLVGMVAGKIGGKGGGRPDMAMGGGVDAA
                     ALPGAVAGVEAWVRERLA"
BASE COUNT          632 a         1121 c         1046 g          667 t
ORIGIN      
        1 gcccgcgatc aggcggcgac tttcgccgag tattccttga ccaggcgcgt gacggccggc
       61 gacagttgcg caccgttgtc cgaccagtac tgcacgcggt caagcgaaat acgcagattt
      121 tcctgccctt cattgccaac cgggttgtaa aaacccacgc gttcaatgaa acggccatcg
      181 cgccggttgc gcgaatcagc agccacaagg ttgtaaaagg gacgtttctt cgagccacca
      241 cgggcaagac gaatcaccac cataggtaat cctttgataa tgttttgaaa aacaaaagat
      301 tctagcatct tgcggccatg caaggcaaac gcaagacatc caatccgcca caaatcccgc
      361 ccaatcggaa tcaacgtgtt tcgtcaacgc ctggccaggt aatgctccat gctcgcgccc
      421 ttatcgacct gcgccagcaa cgtgttgccg gtctggcggg cgaaggcctg gaaatcggtc
      481 acggcgtggg tatcggtggt gatcactttg agcaccgcgc cgctttccaa ccgagccagc
      541 gctttcttcg cccgcagtat gggcaatggg catttcaagc ccgaggtgtc gatttccagg
      601 gtgggggccg gaagatcccc cgtgttgcca tccatcatga tgcctagtct agtgtgctgt
      661 ttgattaatt cgactactca acttcggcga ctggactagc caaacagcac actagtccac
      721 cgcggcagcc accccaaaag ggatggcccg gaatccattc aagccaggcg ttcacgcacc
      781 caggcttcca cgcccgccac ggcacccggc agcgcggcgg catccacgcc accgcccatc
      841 gccatgtcgg ggcgcccgcc ccccttgcca ccgatcttgc cagccaccat gccgaccaga
      901 tcgcccgcct tcacacgcga cgacaggtcg ggcgtcacgc cagcgaccag gctgatcttg
      961 ccttcggcct cggccgtcag cagaacgatg gccgacttga gccggttctt gagctgatcg
     1021 accatgctgc gcagtatctt ggggtcggtg cccgtgagag tgccgaccag cagtttggaa
     1081 tccccttgca gcacaatcgc ggcccgatcg gccagatcat tgccggcctg caccgccagc
     1141 ttggtgcggg cttgctcgag atcgcgttcg gctgttttca actgagcctg cagttgcgcg
     1201 atgcgttcgc caagctcggc gggctgaacc ttgagttgat cggccgcatg ggccagcgtg
     1261 ccgctggttt cctgcaccca ggccagcgcg ttctcgcccg tgactgcttc gacacggcgc
     1321 acgcccgccg ccacgccacc ctcggaaatg atcttgaaca ggccgatgtc gcccgtgcgc
     1381 gccacgtggg tgccgccgca cagctcgcgt gaaaaaccaa tatccagcac ccggacggtg
     1441 tcgccgtatt tttcgccgaa cagcgccatg gccccgcctt cgaccgcctg gtcgtagggc
     1501 atgacgcgtg tggcgacggc ctggttggcg aggatctgtt cattgacgat gcgctcgacc
     1561 cgggcgatgt cgtcggacga aatgggcgcg tcgtgcgcga agtcaaagcg cgtcttgtcg
     1621 ggatcgacca gggaaccgcg ctgctgcacg tgatcgccca gcacctggcg caatgccttg
     1681 tgcatcaggt gcgtggccga gtggttgcgc atggtgcgtt ggcggcgtgc gccatcgact
     1741 tgcgcgtcga gcgaatcgcc cactttcagc gaaccgcttt ccaggacgcc atggtggccg
     1801 aagacattgt tctggatctt ctgcgtgtcg gagaccgtga agcggacgcc accagccagc
     1861 aattccccgg catcgcccac ctgcccaccc gattccgcat agaacggcgt ggcgtccagc
     1921 accacgatgg cttgctggcc gggcgacacg gtttccaccg ccgcgccatc cacgtacagc
     1981 ccggtgatgt ttcccgttcc ccgcagatgc tcgtaaccgt cgaagcgggt ggccgcgccg
     2041 tggtaatcga gctcatcggt cgccttgaac ttgccggcgg cacgggcttg gtcacgctgg
     2101 tgcgccatgg cgtggtcgaa gccttcgtga tcgaccccga cttcgcgttc gcggcagatg
     2161 tcggccgtga ggtcgaccgg aaacccgtac gtgtcgtaga gcgtgaacaa ggtatcgcca
     2221 tcgaggacgt cacccttcct cactccgacc agcgccgact ccaggatctt cagcccattc
     2281 tccagggttt cgctgaaccg ctcttcttcc tggcgcagca cctgctccac ccggttttgc
     2341 agccgggcga gctcggggta cgccgcaccc atttcctgag ccaggtcggc gaccaggcga
     2401 tgaaagaacg gtttggtctg acccagcttg tgcccatgac gcaacgcgcg gcgcacaatg
     2461 cgccgcaaca catagccacg cccctcatta cccggaatga tgccgtcgac gataaggaag
     2521 ccgcatgccc ggatatggtc ggcgatgacc ttgagcgagc tgttggccag atccgacgtg
     2581 ccggtctcgc gcgccgcggc gcggatcagg ttctggaaaa gatcgatgtc gtaattggaa
     2641 tgcacgtgct gcagcacggc cgcgatgcgt tccaggccca tgcccgtgtc gacacagggc
     2701 ttgggcaggc gcggcatgtt gccttcggcg tcgcgctcga attgcatgaa caccaggttc
     2761 cagatctcga tgtagcgatc gccgtcttcc tcgggcgacc ccggggggcc gccccagatt
     2821 tccgggccgt ggtcgtagaa gatctcggaa cacggaccgc agggaccagt gtcggccatc
     2881 tgccagaaat tgtcggacgc gtagcgcgcc cccttgttgt cgccgatgcg gatgatgcgc
     2941 tcgggcggca cgccgatttc gtcggcccag atgccatagg cctcgtcgtc ttcctggtag
     3001 accgtcaccc agagcttctc agtcggcagc ccgtatacct tggtcagcag ctcccaggcg
     3061 ttcgtgatgg cctcgcgctt gaagtaatcg ccgaagctga aattgcccag catctcgaag
     3121 aacgtgtggt gccgcgccgt gtagcccacg ttttccaggt cgttgtgctt gcccccggcg
     3181 cgcacgcagc gctgcgagga ggtggcgcgg ctgtagggcc gggtttcctt gccggtgaac
     3241 acattcttga actgcaccat gcccgagttg gtgaacagca aggtcgggtc gttggccggc
     3301 accagggaag acgacggcac gatctggtgc cccctggact cgaaaaagga gaggaatttc
     3361 tgacggatct cggaggtctt cattgcggaa tggcgcgatt tgaacaggta acgtttgatt
     3421 atagagggag ttggccgggc aacccctatt ccacgcacag gtagcc
//
LOCUS       DASTNK010000006        14382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1117045, whole genome shotgun sequence.
ACCESSION   DASTNK010000006 DASTNK010000000
VERSION     DASTNK010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14382
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117045"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VFR20_00380"
     CDS             complement(<1..659)
                     /locus_tag="VFR20_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEU0228999.1"
                     /translation="MNALDLLWPWLVRHRRRLAGAVLLAAVTVWAGVGLFAVAGWFIT
                     GAFLAGASLAFDLFAPSALVRGLSFLRIGSRYAERVTGHAATLDLLADLRTAVFARVM
                     ALSPAQLARYRDGDLVARLIGDVDTLDTLFLLIVAPALTAIVAGLMFGLLVGVYAPVL
                     AWSVTATLLACALLIPWALARVSRRPGGQVQIEASRARALIHDAVQGHVDIAAFGAQS
                     RV"
     gene            complement(646..2502)
                     /gene="cydD"
                     /locus_tag="VFR20_00385"
     CDS             complement(646..2502)
                     /gene="cydD"
                     /locus_tag="VFR20_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HEU0229000.1"
                     /translation="MPNVQEGASRSPSGADRGQSRWLSSLWRCSRVGFAWAILAPLFA
                     GGLLVAQVWLLAQALGGVVAESLPVGQSTPYILGVVGLILVRAVLSWSGERAAGRGSE
                     TIKRELREALFQRLLALGPRWTRERVSGELGSTIVDQVEHLDGFFTRYLPSSIAAAFL
                     PVAFGVVLLPLDWVAALILLASAPLIPVFMALVGWGAEAASRRHQRELTRLSGLFGDR
                     LRGAFTLKLFGRADAEIQAVRVASVDLSRRNMAVLRIAFLSSAVLEFFAALGVAGVAL
                     YVGLTYLGYVHLRGDALSLPLGLFALFMAPEVYNPLRQFAANYHDRAAARAAVGQIAA
                     MFDGLPAVDPTPAGASTLSYGASESQVSDSMASGRPPYPVAVPGGVPSCADDGARNAG
                     SVGNSIVVHVRDLTIHAAGRERPVLRDASLDIRRGDTVALMGPSGSGKTTLLEALAGL
                     RPVSNGSIRVLGRPVTSGVDPGLGNGVALLDQRPFFSSDSIADNLRMACPDADDARLT
                     RALAQACAADFVATAPDGMQTCLGRDGHGLSGGQLHRLALARVFLLDPDLVLLDEPAA
                     HLDAGTRMRVVDAILAFCAGRTLLIATHDLRIAARMGRVLHIERDGGLLERA"
     gene            complement(2552..2695)
                     /gene="cydX"
                     /locus_tag="VFR20_00390"
     CDS             complement(2552..2695)
                     /gene="cydX"
                     /locus_tag="VFR20_00390"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HEU0229001.1"
                     /translation="MWYFSWILGLGLACAFSVLNAMWFELREGESHNFRNPGMYNDGS
                     DSL"
     gene            complement(2783..3934)
                     /gene="cydB"
                     /locus_tag="VFR20_00395"
     CDS             complement(2783..3934)
                     /gene="cydB"
                     /locus_tag="VFR20_00395"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEU0229002.1"
                     /translation="MEQLIPLDYTTLRVIWWALLGFLLAAFAVMDGFDLGVATVLPLV
                     SRTDVERRVVFNVLGPVWEGNQVWLIVGGGVVFAAWPLLYAMAFSGFYLAMMLLLIAL
                     ILRPICFKYRSKMPDARWRSTWDALLCLCGLVAALVFGVAVGNVIVGVPFVFALDTMR
                     PIYQGGFFGLFMPFPVLCGVISVCMLAMHGAVLLAWKTEGLVAVRARRCVRVFGVITV
                     VLFILAGVWVARGVDGFSIVGAVHTGLASNPLDKAVAVTSGGWMRNYARWPLIWIAPA
                     MGVAGALLAVGLAGLRSCAPAFLASSISVVGVVWTFGLSLFPFLLPSSVAPDVSLTVW
                     DASSSHMSLWIMLLATCLLLPLVMLYTAWVYRVMRGKVTDKTVGGGHAY"
     gene            complement(3946..5544)
                     /locus_tag="VFR20_00400"
     CDS             complement(3946..5544)
                     /locus_tag="VFR20_00400"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEU0229003.1"
                     /translation="MFNLTVVDLSRFQFAATVMYHFLFVPLTLGLSFLIAIMESVYVM
                     SGRDIWRRMTVFWGGLFGINFALGVATGVVMEFQFGMNWAYYSHYVGDVFGAPLAIEG
                     LMAFFLEATFVGLFFFGWDRLSKVGHLTVTWLVALGTNLSALWILIANGWMQNPVGAV
                     FNPDTMRMEMTDFATVIFNPVAQAKFVHTVSAGYVMGAVFVMGVSAWYLLRGRHSELA
                     KRSMVVAASFGLAAALSVVVLGDESGYLTTEHQKMKIAAIEAMWQTEPAPASFNLFAI
                     PDQKHHTNHFAVEIPYVMGIIGTRSLTTPLLGINDLVKRAEVRVKNGILAYKALQKVR
                     ADRDDKVARAKFDAHWRDLGYALLLKRFTPDIAKATPQDIVKAAESTVPQVAPLYWTF
                     RIMVGLGFYFIFFFAFAFWLASTGRLAQHPRFLRIALWTLPLPWLAIESGWFVAEFGR
                     QPWVIEGVLPTFYAASGLTVADLATSLTIFAVLYTALAIVGGKVMLRAIRSGPTEPAG
                     SPRDDAPPAAIPGSTTAATAPRAI"
     gene            complement(5672..6211)
                     /locus_tag="VFR20_00405"
     CDS             complement(5672..6211)
                     /locus_tag="VFR20_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HEU0229004.1"
                     /translation="MKLGPQAERFILHFGEMGGRWGVNRTVGQIYALVFLSPRALHAD
                     EIAETLGFSRSNVSMGLRELQSWRLVHMVHQIGDRRDYFQAPDDVWEIFRVLAEEKRR
                     REIDPTVTMLRDVLLEEPTTEDEIHGQKRINEMLELVELSTAWFDEVHRLPPETIKSL
                     MRLGTKVQKVLGLAGKLRK"
     gene            6346..7335
                     /locus_tag="VFR20_00410"
     CDS             6346..7335
                     /locus_tag="VFR20_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEU0229005.1"
                     /translation="MENPARQVPASAIDSAHRTFRTEIIALQALDARLDGAFEQAVAL
                     ILECRGRIVATGIGKTGHIARKLAATLASTGTPAFFMHGTEAVHGDLGMLTGEDILLA
                     ISYSGTGQELLTLVPPTKRLGARLIAITGNPDSELARAADVHLNAHVDQEACPLNLAP
                     TASTTAALVLGDAIAVACLEARGFDADDFARSHPGGALGRRLLTHVRDVMRIGDELPL
                     VDIATPVTQALAVMSEKRMGMAIVVDDAGHPVGLFTDGDLRRLIARDGDIRPLTVSDG
                     MTHSPRTISPNALAAEAAARMDTGRLNQMLVVDDTGLLLGALHMHDLLAAKVI"
     gene            7338..7937
                     /locus_tag="VFR20_00415"
     CDS             7338..7937
                     /locus_tag="VFR20_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEU0229006.1"
                     /translation="MGTPHTPHPAEAMLLAKVAPALLERLRAVRLMAFDVDGILTDGS
                     LWYGESGEMLKRFNALDGHGLRLLGEGGIVVALVTGRQGPIISRRAADLGISIVHQSV
                     RDKSGTLDTLAREHGFTINQVGYMGDDIIDLPAMQRAGFAASVPNAPAYVAQAAHWVA
                     SHSGGAGAVRECCDLILAAQGRLGACLAPNAFILRGMSQ"
     gene            7942..8586
                     /gene="lptC"
                     /locus_tag="VFR20_00420"
     CDS             7942..8586
                     /gene="lptC"
                     /locus_tag="VFR20_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEU0229007.1"
                     /translation="MKERAPAIIAVVLLLSLVLGTWWAAEYAQKSIPIDPPARITHDP
                     DSWAENFVMIRTDLKGVAINRLEGNYLQHYPDDDSYKITAPTAIGQRPGDPITVGTSR
                     TATMDKNGTRIVMIGDAHVHRRSDKDHAPLDVTSQQLTLLPNQDVVFTDHPAVVINGN
                     SKMHGKGMRYNNKTQVLQVFSASDVKISGADVAKRRAANSDQPAKPTNNPAKTP"
     gene            8583..9350
                     /gene="lptA"
                     /locus_tag="VFR20_00425"
     CDS             8583..9350
                     /gene="lptA"
                     /locus_tag="VFR20_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEU0229008.1"
                     /translation="MKSSSFSRFAPWLASGFALILQAGMPVWAASPANAASMTAPSAA
                     TTPSTAKSQGQDKNTAGKPAAEPDTVILSDTLHYDDKDKTSVFTGNVLMTRGQMTLRA
                     DKLVAHQDAQGNQSGTATMNTPGKLVYVREDNPLKFEVVEARGLRAQYDGKTSEIEMI
                     GQAVVTRFICGTAFDKISGERVRYNQKTDVYEAFSGPQSPTRDRRVRSVAQPQAKIDA
                     AIAECRKKSGPATPAHKTDAHGNPHHETPHQGKTAKN"
     gene            9389..10183
                     /gene="lptB"
                     /locus_tag="VFR20_00430"
     CDS             9389..10183
                     /gene="lptB"
                     /locus_tag="VFR20_00430"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEU0229009.1"
                     /translation="MPDNATPKPGTLRPPSSVHGSLRATGLRKTYGKRTVVHDVSLSV
                     DSGEVVGLLGPNGAGKTTSFYMIVGIVPTDAGRIEIDDTPITSMPMHKRARMGLSYLP
                     QDASVFRRLTVEENIRAVLELQIDDDGRPYSSAKIGQNLDTLIDELQIDHIRRNTAIS
                     LSGGERRRVEIARALATHPRFILLDEPFAGVDPIAVIEIQRIVHFLKGRNIGVLITDH
                     NVRETLGICDRAYIISEGKVLTDGHPREIIDNDAVRKVYLGKNFKM"
     gene            10427..10753
                     /gene="raiA"
                     /locus_tag="VFR20_00435"
     CDS             10427..10753
                     /gene="raiA"
                     /locus_tag="VFR20_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014906047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU0229010.1"
                     /translation="MNLSITGRHLEVTPAIHEYVLNKLARVARHFDNVIDTQVMLSVE
                     RLKHSAEVTMRLRGKDIHCEATDDNLYSAIDLLADKIDRQVIKYKTKVQNHAHEPVKR
                     QEATVE"
     gene            10921..11385
                     /locus_tag="VFR20_00440"
     CDS             10921..11385
                     /locus_tag="VFR20_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU0229011.1"
                     /translation="MNLMSRILPPRNVVLDLEATSKKRAFEQAGLLFENHHGIARTAV
                     FHSLIARERLGSTALGHLIAVPHGRLKNLKEPCAAFFRLAHPVRFDASDGRMASLLFF
                     LLVPESTTQMHLDMLAEIARLTSDAALRQALATETDSAAVHRLLTTSLTETD"
     gene            11395..12324
                     /gene="hprK"
                     /locus_tag="VFR20_00445"
     CDS             11395..12324
                     /gene="hprK"
                     /locus_tag="VFR20_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEU0229012.1"
                     /translation="MLTIQELVGDNADKIPFTWVAGTGAAHRLIPDTGMAAADLVGHL
                     NLIHPSRIQVFGKEELSYYLRFESKRRHHHLEDLVTGGVPAILIAENLQAPEDLQEFC
                     NLQQVPLLSTTIEAAQLIDLLRIYLGKRLAPKTTVHGVFMDVLGVGVLIMGESGLGKS
                     ELALELISRGHGLVADDAVELSRSAPHVIEGQCPSLLQNLLEVRGLGLLDIRTIFGET
                     SVRRKMKLKLIVQLVRSNADNFERLPERDETQDILGISIRRVMLQVAAGRNLAVLVEA
                     AVRNTILTLRGIDTMGEFIERQALAIMQNSRPD"
     gene            12326..13198
                     /gene="rapZ"
                     /locus_tag="VFR20_00450"
     CDS             12326..13198
                     /gene="rapZ"
                     /locus_tag="VFR20_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU0229013.1"
                     /translation="MLNIVLITGISGSGKSVALRFLEDAGYTCVDNLPVRFLHEFIAD
                     TRENELERVAISIDVRTPGELAGLPDVITSLRARGTVFKVIFLDASDHTLLQRYSESR
                     RRHPLTDKLQRGGTPPSLQECINTERELLAPLRNQEHVIDTSDLTPGQLRTWMRDLIQ
                     TDRAPVVLTFESFAYKRGVPGDADLVFDVRCLPNPHYDRTLRPLTGRDEPVAQWLRQF
                     GSVETMIDDIAGFIRRWLPLYMQDTRNYLTIAIGCTGGQHRSVYVVEQLARKFADHSP
                     LLIRHRNQLFNRNP"
     gene            13195..14298
                     /locus_tag="VFR20_00455"
     CDS             13195..14298
                     /locus_tag="VFR20_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEU0229014.1"
                     /translation="MTRRRSYSIALTFLSLLILAGCSTPGKYARGGYYQNDGPGANPP
                     ANVASLPDAVPRVETYLSSTSKPYEVFGKEYVPVKPDQPYKKRGTATWYGRQFNGQKT
                     ASGERYNMYAMTAAHPTLPIPSYARVTRVSTGKSIVVRINDRGPFHSNRIIDLSYAAA
                     AKLGLIRHGSDQVLVQAITNDDIRRGDQDLASNAPQSAPPTISSTTRSTTPDVLAALG
                     EPRGTISVAHPATGADIKRADRARAGSTSSSGAEQLAYADVPSDTGDSGNASWSANTV
                     RDTTSAPGNASRSNGGVYLQFGAFSGEQNAQHLASKLNRQMAKMDSPPATVDPGAHLY
                     RVQVGPYPNRTAAAHAARRIHEATGSTSTVTER"
BASE COUNT         3093 a         4933 c         4170 g         2186 t
ORIGIN      
        1 acgcgtgact gggcaccgaa ggccgcgatg tcgacatgcc cctgcaccgc gtcatggatg
       61 agggcacgcg cgcgcgacgc ttcaatctga acctgcccgc cggggcgacg cgacacgcgt
      121 gccagagccc aggggatcag cagggcgcac gccagcaagg tggcggtgac cgaccacgcc
      181 aacactggcg cgtacacacc gaccagcagg ccgaacataa ggccggcgac gatggcggtc
      241 agcgcaggcg ccacgatgag caagaacaag gtgtccaggg tgtccacgtc gccgatcagg
      301 cgggcgacca gatcgccgtc gcggtaacgg gcgagctgcg ccgggctcaa ggccatgaca
      361 cgggcgaaca ccgcggtgcg taaatcggca aggagatcca gggtggcggc atggccggtg
      421 acacgctcgg cataacggga accgatgcgc aggaaagaaa ggcctctcac caacgcggag
      481 ggagcgaaca ggtcgaaagc caaggacgca cccgccagaa aggcgccggt gataaaccag
      541 cccgccaccg cgaacagacc cacaccggcc cagaccgtga cagcggcgag caacacggcg
      601 ccggccagac ggcgtctatg ccggaccagc cagggccaca acagatcaag cgcgttcaag
      661 caggcctcca tcgcgttcga tgtgcaacac acgccccatg cgtgcggcga tgcgcaggtc
      721 gtgcgtggcg atcagcaagg tacgacccgc gcaaaacgcc aggatcgcat ccacgacccg
      781 catcctggtc cccgcatcca ggtgcgcggc cggctcatcc agcaacacca ggtcagggtc
      841 cagcagaaag acccgggcca gcgccaatcg atggagctgg cctccagaca ggccatgacc
      901 atccctcccc aggcacgttt gcattccgtc gggggcggta gccacgaagt cggcggcaca
      961 cgcttgcgcc agagcccggg tcaaccgggc gtcgtcggcg tccgggcagg ccatgcgcag
     1021 attgtcggca atggaatcgg aggaaaagaa tggacgctgg tcaagcaggg cgacgccgtt
     1081 ccccaggcct ggatcgaccc cgctcgtcac tggccggccc agcacgcgga tgcttccgtt
     1141 cgacaccgga cgcaagcccg ccagcgcttc cagcagcgtt gtcttccccg atccgctcgg
     1201 tcccatgagg gcgacggtat cgccacgccg gatatccagt gatgcgtcgc ggagcaccgg
     1261 acgctctcta cccgccgcgt gaatcgtgag atcgcgcacg tggacgacaa tggagtttcc
     1321 cacactcccc gcattccgcg cgccatcatc ggcgcatgac ggaaccccgc ccggaaccgc
     1381 tacgggatac ggtggacgac ccgaggccat cgagtcggaa acctggctct ccgatgcgcc
     1441 ataggaaagc gtcgacgcgc cggcaggcgt cggatccacg gcgggcaggc cgtcaaacat
     1501 ggcggcgatc tgccccaccg cggcgcgcgc ggcggcgcga tcatgatagt tggcggcgaa
     1561 ctggcgcaag gggttgtaga cctccggcgc catgaacagc gcgaacaacc ccaggggcag
     1621 gctcaaggca tcccctcgta gatgaacata gcccagatag gtcaacccca catagagcgc
     1681 gacccccgcc acgcccagcg cggcgaagaa ctccagcacg gcggaggata gaaacgcaat
     1741 gcgcagcacc gccatgttgc gccgactgag atccacgctg gcgacgcgca cagcctgaat
     1801 ctcggcgtcg gcgcgaccga aaagcttgag cgtgaacgcc ccgcgcaacc gatcgccaaa
     1861 caaccctgac agtcgggtca gctcacgttg atgccgccgg ctggcggcct cggcccccca
     1921 acccaccagg gccatgaaca cgggaatgag gggcgcgctc gcaagcagaa tgagcgcagc
     1981 cacccagtcg agcggcagca acaccacacc gaacgcgacg ggcaagaagg ccgcggcaat
     2041 ggacgatggc agatagcgag tgaagaagcc atccaggtgc tcgacctgat ccacgatggt
     2101 gctgcccagc tcacccgaaa cacgctcacg cgtccagcgt ggccccagcg ccagaagacg
     2161 ttgaaataat gcctcgcgca gttcgcgctt gatggtttcg ctgccgcgac ccgccgcgcg
     2221 ctctcccgac cacgacagca cggctcgaac cagaatcagg cctacgacac ctaatatgta
     2281 cggggtggat tggccgaccg gcaggctctc ggcgaccacc ccgcccagtg cctgggcaag
     2341 cagccagacc tgcgccacca gcagcccccc tgcgaagagc ggagccagga tggcccaggc
     2401 gaacccgacg cgtgaacacc gccacaggga ggacaaccag cgggactgtc cgcgatcggc
     2461 cccgctgggc gaccggctcg cgccttcttg cacattcggc atggcgtcgc ctcaagccct
     2521 cgaccgtcgc atcactcgcg gcaccaccgc atcacaacga gtccgagcca tcgttgtaca
     2581 tgcccggatt acggaaatta tgcgattcgc cctcgcgcag ctcgaaccac atggcgttca
     2641 ggacggagaa cgcgcaggcc agacccagcc ctaatatcca ggaaaaatac cacatgtgtc
     2701 acctcgacaa ataaccggac ggttgcccga agaacctttc attgcacacg tgccgtgccc
     2761 cacaccccac atgtgcggca catcaatagg catgcccgcc acccacagtc ttgtccgtca
     2821 ccttgccccg catcacccga tacacccagg cggtgtagag catcacgagt ggcagcaaca
     2881 ggcaggtggc cagcaacatg atccagagcg acatatggct ggacgacgcg tcccacacgg
     2941 tgagactgac atccggcgcc actgaagaag gcaagagaaa cgggaacagg ctcaggccga
     3001 atgtccagac gacaccgacc acgctgatcg atgaggccag gaacgccggc gcgcacgagc
     3061 gcaagcccgc cagccccacc gcgagcagcg cgcccgccac acccattgcc ggcgcgatcc
     3121 agatgagggg ccagcgggca tagttgcgca tccagccgcc cgacgtcacc gccaccgctt
     3181 tatccagggg gttggaggcc agaccggtgt gcaccgcgcc gacgatcgag aacccgtcca
     3241 cgccacgcgc cacccacaca ccggccagga tgaacaacac gacggtgatc accccgaaca
     3301 cccggacaca gcggcgggcg cgaaccgcca ccagcccctc ggtcttccag gccagcaaca
     3361 ccgcgccatg catagccagc atgcagacgc tgatcacgcc gcacaagacg ggaaacggca
     3421 tgaacaaccc gaagaaaccg ccttggtaga tgggccgcat ggtgtcgagc gcgaacacga
     3481 acggcacacc cacgatcacg ttacccaccg ccacgccgaa caccaaggcc gccaccagcc
     3541 cgcacagaca cagcagcgcg tcccaggtgg accgccagcg cgcatcgggc atcttgctgc
     3601 gatacttaaa gcagatgggg cgcaggatca gcgcgatcaa caacagcatc atggccagat
     3661 agaaacccga gaacgccatg gcgtacagca gcggccatgc cgcgaacacc acgccgccgc
     3721 ccacgatcag ccacacctgg ttgccttccc ataccggacc gagcacattg aaaaccacgc
     3781 ggcgctcgac gtcggtgcgc gacaccagcg gcagcaccgt ggccacgccc agatcgaaac
     3841 catccatgac cgcgaaggcc gccagcagga aacccagcag cgcccaccag atgacgcgca
     3901 gcgtcgtgta atcgagggga atgagttgtt ccatgaaggg tctcctcaaa tggcgcgcgg
     3961 cgcggtggcg gcggtggttg atcccggaat ggcggcgggg ggcgcgtcat ccctcggcga
     4021 acccgcgggt tccgtaggcc cggaacggat ggcgcgcagc atgactttcc cgccgacgat
     4081 ggccagggcg gtgtacagca cggcgaagat ggtcaggctg gtggccaggt cggcgaccgt
     4141 caaaccggaa gccgcataga aggtggggag caccccttct atcacccagg gttggcgccc
     4201 gaactcggcc acgaaccagc cagactcgat cgccagccac ggcaggggca gtgtccacaa
     4261 agcgatccgc aggaaacgcg gatgctgggc caggcgcccc gtggaggcca gccagaacgc
     4321 gaaggcgaag aaaaagatga aatagaaacc aagccccacc atgatccgga acgtccagta
     4381 caaaggcgcc acctgcggca cggtgctttc cgccgccttg acgatatcct gcggcgtggc
     4441 tttggcgatg tcaggcgtga agcgcttgag caacagcgcg tagccaagat cccgccagtg
     4501 cgcatcgaat ttcgcgcgcg ccaccttgtc gtcccgatcc gcccgcactt tctgcaatgc
     4561 cttgtaagcg aggatcccat tcttgacgcg cacctccgcg cgcttgacca ggtcattgat
     4621 gcccagcagc ggggtggtca gggaacgggt gccgatgatg cccatgacat aggggatctc
     4681 gacagcgaaa tggttggtgt gatgcttctg gtcggggatc gcgaacagat tgaaactggc
     4741 cggcgccggc tcggtctgcc acatcgcctc gatagcggcg atcttcattt tctgatgctc
     4801 ggtggtcagg taaccgcttt catcgcccag cacgaccact gacagcgcgg ctgccagccc
     4861 gaagctggcc gccaccacca tggaacgctt ggccagttca ctgtgacggc cacgcaacaa
     4921 gtaccaggcg ctgacaccca tcacgaacac ggcgcccatc acatagccgg cgctgaccgt
     4981 gtggacgaac ttggcctggg caaccggatt gaaaatgaca gtggcgaagt ccgtcatctc
     5041 catgcgcatg gtgtccggat tgaacacagc ccccaccggg ttctgcatcc agccattggc
     5101 gatgagaatc cagagcgcgg aaaggttggt tcccagcgcc accaaccagg tcacggtcag
     5161 gtgccccacc ttcgagaggc ggtcccaacc gaagaagaac aatccgacga acgtggcttc
     5221 gaggaagaac gccatcagac cctcgatggc caacggcgca ccgaaaacat cgcccacata
     5281 atggctgtag taagcccagt tcatgccgaa ctggaactcc atgaccacgc cggtcgccac
     5341 gcccagcgcg aaattgatgc cgaacagccc cccccagaac accgtcatgc gacgccagat
     5401 gtcgcgaccg ctcatcacat agacgctctc catgatggcg atgagaaacg acagccccag
     5461 cgtcaagggc acgaaaagaa agtggtacat caccgtcgcg gcgaactgga accgcgacaa
     5521 gtccacgact gtcagattga acatgaagcg ctcctgcgca tgatttatta ccgaactatt
     5581 tattaccgta ttatttatta ctgcactatt tattgtgatg acgacgcctt gcgcaaaacc
     5641 gtgtggcggg tgacaacacc ggccgcgccg gctatttccg caacttgcca gccagcccca
     5701 gcactttctg caccttggtg cccagacgca tgaggctttt gatcgtctcc ggcggcaggc
     5761 ggtggacctc gtcgaaccag gccgtgctga gctcgacgag ctcgagcatt tcgttgatgc
     5821 gcttctgccc gtggatttcg tcttccgtgg tgggctcttc gagcaggacg tcgcgcagca
     5881 tggtcacggt gggatcgatc tcgcgccgac gcttttcctc ggccaggaca cgaaaaatct
     5941 cccatacatc gtccggcgcc tggaagtagt cgcgccggtc accgatctga tgaaccatgt
     6001 gcaccagccg ccaggactgc agctcgcgca accccatgct cacattggag cgggagaaac
     6061 ccagggtctc ggcgatttca tcggcgtgca gcgcgcgggg cgacaggaaa accagggcgt
     6121 atatctgccc caccgtgcgg ttcacgcccc agcgcccccc catttccccg aaatgcagga
     6181 tgaaccgttc cgcctgtggg ccgagcttca tgaagttttc caattttctg ttattgcggt
     6241 aatttcagta attactgaaa tcaaagtaac gcaagaatac tccaagcaca gcatgcatgg
     6301 caagcgaacc catgcccttg cccgtactca cgttaaaatc ggacaatgga aaaccccgcc
     6361 cgccaagtcc cggcctccgc gatcgactcc gcgcatcgca ccttccgcac cgaaatcatc
     6421 gccctgcaag cgctcgacgc gcgtctcgac ggcgcgttcg aacaagcggt cgcattgata
     6481 ctcgaatgcc ggggccggat cgtcgccact ggcataggca agaccggcca catcgcgcgc
     6541 aagctggcgg ccacactggc ctccaccggc acgccggcgt tcttcatgca cggcacagaa
     6601 gccgtgcatg gcgatttggg catgctgact ggcgaagaca tcctgttggc catttcttat
     6661 tccggcaccg gtcaggaact cttgacgctg gtccccccca ccaaacggct gggggccagg
     6721 ctcatcgcca tcaccgggaa tcccgactcc gaactggccc gcgccgcgga tgtgcacctg
     6781 aacgcccacg tcgaccagga agcctgcccg ctgaatctcg cccccaccgc gagcaccacc
     6841 gccgcactgg tgctgggcga cgccatcgcc gtcgcctgcc tcgaggcgcg cggtttcgat
     6901 gccgacgatt tcgcccgatc ccacccgggc ggcgccctgg ggcgtcgact cctgacacat
     6961 gtgcgggacg tcatgcgcat cggcgacgaa cttcccttgg tcgacatcgc cacccccgtc
     7021 acgcaggcgc tggccgtgat gtccgaaaag cgcatgggca tggccatcgt cgtggacgac
     7081 gcgggccacc ccgtcggtct cttcaccgat ggcgatctgc gacgactcat cgcccgtgac
     7141 ggcgacatcc gtccactgac cgtgagcgac ggcatgaccc actcgccccg gaccatctcc
     7201 cccaacgcat tggccgccga ggccgcggcg cgcatggaca ccggccgcct gaatcaaatg
     7261 ctcgtggtgg acgataccgg gctattattg ggggctttgc atatgcacga tctgcttgcc
     7321 gcgaaggtga tctgaacatg ggaacacccc acaccccaca cccggccgaa gccatgcttc
     7381 tggcgaaagt cgccccggct ctccttgaac gcttgcgcgc cgttcggctg atggcgttcg
     7441 acgtggacgg catactcacc gacggtagcc tgtggtatgg tgaaagtggc gaaatgctca
     7501 agcgtttcaa cgccctcgac ggccacggcc tgcgtctcct gggcgaaggc ggcatcgtgg
     7561 tggcgttggt caccgggcgc cagggcccca tcatctcgcg ccgcgccgcg gatctgggca
     7621 tctcgatcgt gcaccaaagc gtccgcgaca agtccggcac gctcgatacg ttggcccgcg
     7681 agcacggttt caccatcaac caggtcggct atatgggcga tgacatcatc gatctgcccg
     7741 ccatgcagcg cgctggcttt gcggccagcg tgccgaacgc ccctgcctac gtggcgcaag
     7801 ccgcccactg ggtcgcgtcg cacagcggtg gcgcgggcgc cgtccgcgag tgctgcgacc
     7861 tcatcctcgc ggcccagggg cggctcggcg cctgtctcgc ccccaatgca ttcatcctca
     7921 gagggatgag ccagtaaccc catgaaggaa cgcgcacccg ccatcatcgc cgtcgtacta
     7981 ttgttgtcac tggttctggg gacctggtgg gcggccgagt acgcgcaaaa gtccattccc
     8041 atcgacccgc cggcccgcat cacccacgac cccgactcct gggcagaaaa tttcgtgatg
     8101 attcgcaccg acctgaaagg agtggccatc aaccgcctcg agggaaatta cctacaacat
     8161 tatccggacg acgactcata caagatcacc gcgcccaccg ccataggaca gcgccccggc
     8221 gaccccatca cggtgggcac atcgcgcacc gccaccatgg acaagaacgg aacgcgcatc
     8281 gtcatgatcg gtgacgcgca tgtgcatcgg cgcagcgaca aggatcatgc ccccctcgat
     8341 gtcaccagcc agcaactgac gctgctgccc aaccaggacg tggtgttcac cgaccaccca
     8401 gcggtggtca tcaacggaaa ctcgaaaatg cacggaaagg gcatgcgata caacaacaag
     8461 acccaggtac tgcaggtgtt ttccgcaagc gacgtcaaga tatcgggtgc cgatgtcgcc
     8521 aagaggcggg cggccaattc cgaccagccg gcaaaaccca cgaacaatcc agccaagaca
     8581 ccatgaaatc atcgtctttt tcccggttcg cgccctggct tgcgtcaggc ttcgccttga
     8641 tcctgcaggc cggaatgccc gtgtgggcgg ccagcccggc gaacgcggca agcatgacgg
     8701 cgccgtccgc ggcgaccacc ccttcgacag ccaaatccca aggccaggac aagaacacgg
     8761 ccggaaagcc cgcagcggaa cccgacaccg tcatcctgtc cgacaccctg cattacgacg
     8821 acaaagacaa gaccagcgtc ttcacaggca acgtgttgat gacccgtggc cagatgacgc
     8881 tgcgcgccga caaactcgtc gcgcaccaag acgcccaggg caaccaatcc ggcaccgcca
     8941 ccatgaacac acccggcaag ctggtttacg tgcgtgaaga caaccccctg aaattcgaag
     9001 tcgtcgaagc ccgcgggttg cgcgcccagt acgacggcaa gaccagcgaa atcgagatga
     9061 tcggccaggc ggtggtgacc cgtttcatct gcggcacggc gttcgacaag atcagcgggg
     9121 aacgcgtgcg atacaaccag aagaccgatg tctacgaagc gttcagcggg ccgcaatccc
     9181 ccacccggga ccggcgtgtc cgttccgtgg cccagccaca ggccaagatc gacgccgcca
     9241 tcgccgaatg ccggaagaaa tccggtccgg ccaccccggc ccacaagacg gacgcccacg
     9301 gaaacccgca ccacgaaaca cctcatcagg gcaagacagc caaaaactga ctccgcctcc
     9361 ccgcgcccgc ccccacccct tgcgattcat gccagacaac gccacaccga agcccggaac
     9421 cctcagaccc ccatccagcg tgcatggcag cctgcgcgcc acgggattgc gtaaaaccta
     9481 cggcaagaga acagtcgtgc acgacgtttc gctctctgtg gacagcggcg aggtggtggg
     9541 gttgctgggt cccaatggcg cgggcaagac gaccagcttc tatatgatcg tcggcatcgt
     9601 cccgaccgac gccggccgca tcgagatcga cgacacgccc atcaccagca tgcccatgca
     9661 caaacgggcc aggatgggtc tgtcgtacct gccgcaggat gcctcggtgt tccgacgcct
     9721 cacggtcgaa gagaacatcc gcgccgtgct ggaactgcag atcgacgacg acggccgccc
     9781 ctattcctcc gccaagatcg gccagaacct cgatacgctg atcgatgagc tccagatcga
     9841 ccatatccgc cgcaacaccg ccatctcgct atcgggggga gaacggcgac gcgtggagat
     9901 cgcgcgggcc ctggccacac acccccgctt catcctcctg gatgaaccct tcgcgggcgt
     9961 ggacccgatc gcggtcattg aaatccagcg catcgtgcat ttcctcaaag ggcgcaacat
    10021 cggggtgctg atcaccgacc acaacgtccg ggaaacgctg ggtatctgcg accgggcgta
    10081 catcatcagc gaaggcaagg tcttgaccga cgggcatccc cgggaaatca tcgacaacga
    10141 cgcggttcgc aaggtttatc tgggcaagaa tttcaaaatg tgacgagcct gctgcccgac
    10201 tgggtcgcgc atccaaatcc ggatgcgtgt cagaccaggc attggcgcgg cacactgtag
    10261 aacgtcaccc cccacgccga cgaccaaaag taaccgcccc gcagggcact tgatttactc
    10321 tccataatct ataaactgaa ctcgatacca actcatcaag gaggtttccc tttccctgaa
    10381 acgcgcgtcg cgcacccata tttgcttttg ataggagagc aacaatatga acctgagcat
    10441 tactggtcga cacctcgaag tcactcccgc catccacgaa tacgtgctga acaagctggc
    10501 ccgagtcgct cgacactttg ataacgtcat tgacacccag gtcatgctct cggtagagcg
    10561 cttgaagcac tcggcggaag tcaccatgcg cctgcgtggc aaagacatcc actgcgaagc
    10621 caccgacgac aatctgtact ctgccattga cttgctggcc gacaagatcg accgccaggt
    10681 cataaaatac aagaccaagg ttcagaacca cgcacacgaa cccgtcaaac gacaggaagc
    10741 cactgtcgaa taacccacaa gccggcacgc gccgcgacac catcgacccc caggggtatc
    10801 agacaggtgg cacggtgacc aaaccgcttg tttcggcctc aatcccgagc aggattgggg
    10861 ccttggtgtt tctgggcgca gccagccagc gaaaaggttt tctccctata atggccctct
    10921 atgaacctga tgtcccgaat cctgccaccg cggaacgtgg tgctcgacct ggaagccacc
    10981 agcaagaagc gcgcgttcga acaggcgggc ctgctcttcg agaaccacca cggcatcgcc
    11041 cggacggccg tcttccatag cctgatcgcg cgtgaacgtc tgggttccac agccctgggg
    11101 cacctcatcg cggttccgca cggtcggctg aagaacctga aagagccctg cgcggcgttc
    11161 ttccgcctgg cccacccggt gcgcttcgac gccagcgacg gacgcatggc cagcctgctg
    11221 tttttcctgc tcgtcccgga aagcaccacc cagatgcacc tggacatgct ggccgaaatc
    11281 gcccgcctga cctccgacgc cgccctgcgc caggcactgg ccaccgaaac agattccgca
    11341 gccgtccatc gcctgctcac cacctcgctg accgaaacgg actaggcccc agccatgcta
    11401 accattcaag aactcgtcgg cgacaatgcc gacaaaatcc cgttcacatg ggtggccggc
    11461 accggcgcgg cgcatcggct gatccccgac acaggcatgg ccgccgccga cctggtgggc
    11521 cacctgaatc tcatccaccc gtcgcgcatt caggtctttg gcaaggagga gctgtcttac
    11581 tacctccgtt tcgaatccaa gcgccgccac catcacctgg aagatctggt cactggtggt
    11641 gtcccggcca tcctgatcgc cgagaatctc caagcccccg aagatcttca agagttctgc
    11701 aacctgcaac aggttccgct gctttccacg accatcgaag cagcccaatt gatcgacctt
    11761 ctgcgcattt acctgggcaa gcgcctggca ccgaaaacca ccgtgcatgg ggtgttcatg
    11821 gacgtcctgg gcgtgggcgt gttgatcatg ggggaatcgg ggctcggcaa gagcgaactc
    11881 gccctggaac tgatttcgcg cggccacggc ctggtggccg acgacgcggt ggaactgtcc
    11941 cgcagcgcgc cccacgtcat cgagggacag tgtccctccc tgctgcagaa cctgctggaa
    12001 gtgcgggggc tggggctgct ggatatccgg accatcttcg gggaaacctc ggtgcggcgc
    12061 aagatgaagc tgaagctgat cgtgcagctt gtgcgctcga acgccgacaa cttcgagcgc
    12121 ctgcccgaac gcgacgagac ccaggacatc ctgggtattt ccatccggcg tgtcatgctg
    12181 caggtggcgg cgggtagaaa cctggccgtg ctggtcgagg ccgccgtgcg caacaccatc
    12241 ctgaccctgc gcgggatcga caccatgggc gagttcatcg aacgccaggc attggccatc
    12301 atgcagaaca gccgcccgga ctgacatgct caatatcgta ctcatcaccg gcatctccgg
    12361 ctccggcaaa tcggtcgcgt tgcgctttct ggaagacgca ggctatacct gtgtggacaa
    12421 cctgccggtg cggttcctgc acgagttcat cgccgacacg cgcgagaacg agctcgagcg
    12481 cgtggcgatc tcgatcgacg tgcgcacacc cggcgaactg gcggggctgc ccgacgtgat
    12541 caccagcctg cgagcccggg gcacggtgtt caaggtgatc ttcctggacg ccagcgacca
    12601 caccctgctg cagcgctatt ccgaatcgcg ccgacgccac cccctgaccg acaaactgca
    12661 acggggcggc accccgcctt cgctgcagga atgcatcaac accgaacgcg aactgctggc
    12721 gccgctgcgc aatcaggaac acgtgataga cacgtccgat ctcaccccgg ggcagttgcg
    12781 cacctggatg cgcgacctga tccagaccga ccgggcacca gtggtgctga cgttcgaatc
    12841 attcgcctac aagcgtggtg tgccaggcga cgcggacctg gtcttcgacg tgcgctgcct
    12901 gcccaacccc cactatgacc gcaccctgcg gccgcttacc ggccgcgacg agcccgtcgc
    12961 ccaatggctg aggcaattcg gcagcgtcga aaccatgatc gacgacatcg ccggcttcat
    13021 tcgccgctgg ctgccgctgt acatgcagga cactcggaac tatctgacca tcgccatagg
    13081 ctgcactggc ggacagcacc ggtcagtcta tgtcgtggag caattggcca ggaagttcgc
    13141 cgatcactct ccactgttga tacgacaccg caaccaactg ttcaacagga acccatgacg
    13201 cgacgacgct cctactccat cgccctgacc ttcctcagcc tgctgatcct cgccgggtgc
    13261 tcgacccccg gcaaatatgc gcgcggcggc tactaccaga acgatggccc gggagccaac
    13321 ccacccgcca atgtcgcctc gctgcccgat gccgtccctc gggtcgaaac ctatctcagc
    13381 agcacgagca agccctacga ggtcttcggc aaggaatacg tgccggtcaa acccgaccaa
    13441 ccctacaaga aacgcggcac cgccacgtgg tacggacgcc aattcaatgg ccagaaaacc
    13501 gcgagcggcg aacgctacaa catgtacgcc atgacggcgg cccatccgac gctgcccatt
    13561 cccagttacg cccgggtgac acgggtttcc accgggaaat ccatcgtcgt ccgcatcaat
    13621 gaccgggggc ccttccacag taatcgcatc atcgacctgt catacgcggc ggcggccaag
    13681 ctggggctca tccggcatgg cagcgaccag gtgcttgtcc aagccatcac gaacgacgat
    13741 atccgcaggg gtgatcaaga cctggccagc aatgcgccac agtccgcgcc gccaaccatc
    13801 agcagcacca cgcggtcgac gacacccgat gtgctggcgg cgctcggcga gccccgaggg
    13861 acgatctcgg tcgcgcatcc ggccaccggc gcggatatca aacgggcgga tcgggcgcga
    13921 gccggaagca cttcgtcgtc gggtgccgag caattggcct acgccgatgt gccaagcgac
    13981 actggggatt caggcaatgc gtcgtggtcc gcgaacaccg tccgcgacac gacttccgcc
    14041 cctgggaatg catcacgctc gaatgggggg gtgtacctgc agttcggggc gttcagcggt
    14101 gaacaaaatg cccagcacct ggcaagcaag ctgaaccggc agatggcaaa gatggacagt
    14161 ccgcccgcca cggtcgatcc gggcgcgcat ctctatcggg tgcaggtcgg cccatatccc
    14221 aaccgcacag cggcagccca tgccgcgcgg cgcatacatg aggccactgg gtcgacctct
    14281 actgtgactg agcgctgacg aaccgtatat tgtttgaacc gttttggcca aggcgattcc
    14341 atttgacttg ccgcagggta tttaacacgc cccagggtgg cg
//
LOCUS       DASTNK010000007        12522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_191395, whole genome shotgun sequence.
ACCESSION   DASTNK010000007 DASTNK010000000
VERSION     DASTNK010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12522
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191395"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1871)
                     /locus_tag="VFR20_00460"
     CDS             complement(6..1871)
                     /locus_tag="VFR20_00460"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEU0229015.1"
                     /translation="MVKTDVAGGAGIMKSDTGGVTPRAKGYPWRKIADAGLDGAGVVL
                     LATEVLVLFAGIVARTVFVHPLIWSTEFARLVFVWLTMVGAVVAVRDDQHMRMTAFTH
                     RLPSSWQGWLGAAADLMQLLAVLAMVEPSLAFLQIEQLSSLSQLGISSAWEAAGLVVG
                     FVLMAAYLVGGLLRRSRGDLWRGGISVVVVIGAAFLAGLALSDNTLFEVVFVFVVLLF
                     SAVLIGVPIAFAFGFCSIFYLLVTGSAPLGVMASQVEQGTSSIILLAVPLFVLLGGLL
                     TVTRLAPAMMRFLSDMFGQVKGGLFYVLIGGILLVSGISGAKTADLAAVAPIVVPEMK
                     RHKIDDGHILAVTASCGAMAETIPPSIVLIIIGAVTSTSITALFEAGLMPAIILAVIL
                     AVLARFWAGGHMATGVRPSAGQVLRSFLYGLPALVLPFVIRFCVIDGIATATEIATIG
                     IVYALLLGWVFYGGFRLSALYASLSKAVGLSGAIMFIIAVATAMSWTLTQSGFAEDIT
                     HLMLALPGGPIGFMLGSIFLMIVLGSFLEGIPILVLFGPILFPIARTLGISDVRYGIV
                     VVLAMGIGLFAPPVGLGYYATGIITEIDPNKGLVKIWGYLAALVAGLLAIALIPA"
     gene            complement(1985..3001)
                     /locus_tag="VFR20_00465"
     CDS             complement(1985..3001)
                     /locus_tag="VFR20_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEU0229016.1"
                     /translation="MTQPHISRRALLQGIAATAAFGMLPRQSDAAQFRWKMATGQEPT
                     HPVNVRAAEAIARIKKETNGGLDIKLYPAAQLGTDKQLIAQTRLGGVQIMNMASSVLA
                     GTIPAAGIVNIGYAFLEPKDVWSAIDGALGHHIANEIDQHGMHQIGLSWENGFRNVTS
                     GTRPVKTPADLRNFKIRVPDAPILSSMFAAFHAAPTSMDFSEVYTSLENHVVDGQENP
                     LPIIYTAKLYDVQKYLSMTEHAWDGYLIIGNKPAFARLPKKWQDIVTEAFSDAGRKER
                     ADIAQLSDSLKGKLAAKGMNIVNVDKSAFRGALVKTDYYPHWKDKFGGKAWALLEKTS
                     GALE"
     gene            complement(3250..4248)
                     /locus_tag="VFR20_00470"
     CDS             complement(3250..4248)
                     /locus_tag="VFR20_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU0229017.1"
                     /translation="MSQTTANKPRLTTSAMIASIAGEGQRTQAAPFGHALVELAERRA
                     DIVGMTADLSKYTDLHVFQKAFPERFFQMGMAEQLLMAAAGGMAKEGFVPFATTYAVF
                     ATRRAYDFIHQIIAEEHLNVKICCALPGLTTGYGPSHQATEDISILRGVPGLTIIDPC
                     DALDIEQAVPAIAAHDGPVYMRLLRGQVPLVLDEYDYRFELGKGSLIRDGRDVLVISC
                     GLMTMRALEAAKALASDSVDVAVLHVPTIKPLDEETILREVKRHGRLVVVAENHSVIG
                     GLGEAVASLLLRSGVAPAFRQIALPDAFLDAGALPTLHDRYGISSEAMARRIKEWL"
     gene            complement(4245..5120)
                     /locus_tag="VFR20_00475"
     CDS             complement(4245..5120)
                     /locus_tag="VFR20_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU0229018.1"
                     /translation="MNTPAVTEVRAGAELRRLADSAYRIRRYALQMGEVQGQGYIGQA
                     LGIADVLAVAYCHALNLRPEDPDWEGRDRFLLSHGHYAIAYYAALIEAGVIPLDELAT
                     YGSDDSRLPMSGMASYTPGMEISGGSLGQGLGIGVGMALGLRQKKNPARVVVSMSDGE
                     LDEGATWEAAMSASQYRLANMIALVDINNQQADGPSRDVLGFEPLVDKWAAFGWFVQR
                     VDGNDLAAVLDAFDRARHHDGDQPRVILFDTLIGKGVPFLEQRERNHFIRVDAPEWRM
                     AIDILDAAQRDGAEK"
     gene            complement(5152..5904)
                     /locus_tag="VFR20_00480"
     CDS             complement(5152..5904)
                     /locus_tag="VFR20_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0229019.1"
                     /translation="MLLKDKIVAISGAASIRGIGLATARLFAAHGATVAILDLDASSA
                     AQAAASLGAGHHVGLACDVADKQACEQAVAAVVERYGRIDVLINNAGITQPLKTMDID
                     PDNWRRVLDVNLGGVLYLSQAVIPHMRARKSGSIACMSSVSAQRGGGIFGGPHYSAAK
                     AGVLGLAKAMARELGPDNIRVNCVTPGLIQTDITGGKLTDAMRVDILKGIPLNRLGEA
                     QDVASAYLFLASDLSSYITGAVIDVNGGMLIH"
     gene            6029..6922
                     /locus_tag="VFR20_00485"
     CDS             6029..6922
                     /locus_tag="VFR20_00485"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0229020.1"
                     /translation="MFQNVPLSMLRAFEASGRTGSFRNAAEELCLSPSAVSHAIRGLE
                     EHLGIRLFLRSTRKIQLTPEGEALFTHIRRGFEDLRHGMELVSARGPTVLRLHSAPSF
                     AAQWLVPRLARFFTEHPRIDLRLAANTDYARFEADEFDLDIVYGQPKSTGTVVVPLGE
                     ELLTPLCSPETATRIKSPKDLIGLRLIQSESKQIRWPTWLEANKLPINTTQGARFDRS
                     FLAIAAAADGLGVALESTRLAERELKNGRLVRPLAGQEHEIRYNGHYLAYSSAAHQRH
                     TIGVFREWLLAELADGSLASQ"
     gene            7008..7226
                     /locus_tag="VFR20_00490"
     CDS             7008..7226
                     /locus_tag="VFR20_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU0229021.1"
                     /translation="MTANLVVRNIEPEVVQALKQAAARHGRSAEAEHREILRAQLTRP
                     ARRSFKDVLGGMPNVGTDEDFNFRNAQP"
     gene            7226..7636
                     /locus_tag="VFR20_00495"
     CDS             7226..7636
                     /locus_tag="VFR20_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU0229022.1"
                     /translation="MYLIDTNILSEARKGVRANAGVRDFFEKQDSQHLFLSVLTVGEI
                     RRGLEHIRCRGDLAQALRLETWLDDLVVEFDDHILTFDMACAQVWGKLMSPNAQHAID
                     KQIAATALIFDLTVVTRNLADFQSTGARLINPFR"
     gene            7844..8680
                     /locus_tag="VFR20_00500"
     CDS             7844..8680
                     /locus_tag="VFR20_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0229023.1"
                     /translation="MTDQPSSTTDPVLRLDGVALGYGDVTVLNGIDMAIAPGRVVAVM
                     GGSGSGKTTLLRAATGQIHPLAGTIQAFGHDVASADTNGLQKLRQRMGVLFQQGALFT
                     DLNVFENVAFPLREHRHLPEDDVTRQVLDKLDAVGLRAAAHLNISEISGGMARRVALA
                     RAIVLEPELILYDEPFAGLDPISLGITAQLIRGMTDRLHCASVLITHDVAESFAIADH
                     VYMVGQGKLIASGTPDELSASTDPYVRQFLDAKPDGPIAFNYPETSRFQQWLSSRLKG
                     RP"
     gene            8677..9462
                     /gene="mlaE"
                     /locus_tag="VFR20_00505"
     CDS             8677..9462
                     /gene="mlaE"
                     /locus_tag="VFR20_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEU0229024.1"
                     /translation="MTLVAPISSIGRFVRLRITGLGAFARFFWTLLARSALVVRRPGL
                     VSQQVHFIGNYSLLIISVSGLFVGFVLGLQGYYTLNRYGSEQALGLLVALSLVRELGP
                     VITALLFAGRAGTSLTAEIGLMKAGEQLAAMEVMAVDPFRRVLVPRFWGGVIAMPILA
                     AVFSMVGILGGWFVGVVLIGIDPGTFWSQMQSGVDVFKDIGNGVFKSIVFGVAVTLIA
                     LYQGWTAKATPEGVARATTRTVVSGALVVLGLDFLLTALMFSN"
     gene            9541..10101
                     /gene="mlaD"
                     /locus_tag="VFR20_00510"
     CDS             9541..10101
                     /gene="mlaD"
                     /locus_tag="VFR20_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEU0229025.1"
                     /translation="MTREKTDFWVGLFVLLGIIALVFLALRAGNLSSFSFAPTYQVTA
                     RFDNVGSLKVRAPVKSSGVVVGRVENIAFDNKLYQAQVTLELQKQYQFDDETSASILT
                     SGLLGEQYVGLTPGGGDKMLAQGSRIKYTQSAIVLEDLISKFLYSSANKQGTASGSSG
                     GGSGGGSGGASSGGSLSAPGAAPAKH"
     gene            10244..11212
                     /locus_tag="VFR20_00515"
     CDS             10244..11212
                     /locus_tag="VFR20_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEU0229026.1"
                     /translation="MNRPSPALPFPPAAQAGRRLARLAPMLAITLVAAGCATVKHPNP
                     NDPWESYNRGMYAFNTKVDDVVFKPIAKGYEAVTPKPVRTCVHNMFNNVEDLWSALNS
                     FIQGRGVDFFNTLGRFLVNTTLGLGGCFDVNSQLGGQRVVNDFGTTLGVWGLGSGPYV
                     VLPILGPSTVRDTAAWATTAAATFSENSQFFSPLAPIMAIRNIPVRNGVLALQVIDAR
                     ANLLDAEKLVNQIALDKYSFIRDAFLQRRHALLESKLHGKSANALPNYGDQALPNYSE
                     PALPKYDDPDKPGYTGVVPVKAQPAAAPAKSGGQAATGAPATSAKH"
     gene            11280..11909
                     /locus_tag="VFR20_00520"
     CDS             11280..11909
                     /locus_tag="VFR20_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0229027.1"
                     /translation="MSSPLALARRVMLAGLLGLGLVAVAQADEPVNANGAPNAFVQAV
                     GDNALQAIKQAHVKQGDVAKINQIVDQYVLPYVDMKKTTRLSAGRYWRQATPKQQAEL
                     VNAFKGTLIRTYSGAFTKVDPTSKLEVLPFRGDPKADDLAVRSTLTQTNSPSINIDYR
                     LEKTAQGWKIYDLNVEGIWLIQNYRNQFAEQITSNGIDGLIQALNNQNK"
     gene            12004..>12522
                     /locus_tag="VFR20_00525"
     CDS             12004..>12522
                     /locus_tag="VFR20_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0229028.1"
                     /translation="MPALSLENISKIYPHRSGGWRKWLSTPASGAGFQALDDVSLSID
                     YGEFFGLLGPNGAGKTTLISILAGLSRATKGRASVCGHDVLTDFKQSRRALGVVPQEI
                     VYDPFFTVRETLRIQSGYFGLRRNDDWIDEVLSNLGLADKADANMRALSGGMKRRVLV
                     AQALVHRPPVIVL"
BASE COUNT         2397 a         4107 c         3748 g         2270 t
ORIGIN      
        1 cgtgctcaag cgggaatcag cgcaatggct agcaggccgg cgactagcgc ggccaggtag
       61 ccccatattt tcaccagccc tttgttcggg tcgatctcgg tgatgatgcc ggtggcgtaa
      121 tagcccaggc ccaccggggg cgcgaacagg cctattccca tcgccaggac gaccacgatg
      181 ccatagcgca cgtcgctgat tcccagggtg cgggcgatcg gaaacagaat cgggccgaac
      241 agcaccagga tggggatgcc ttcgaggaaa ctgcccagca cgatcatcag gaagatggac
      301 ccgagcatga agccgatggg gccgccgggc agcgccagca tcaggtgggt gatgtcctcg
      361 gcgaagcccg actgggtcag tgtccagctc atggcggtgg cgacggcgat gatgaacatg
      421 atggcgcctg aaagccccac ggctttggac aacgacgcat agagggccga gagtcgaaag
      481 ccgccataga acacccaacc gagcagcaag gcgtagacaa tgccgatggt ggcgatttcc
      541 gtcgcggtgg cgattccgtc gatgacgcag aaccggatca cgaagggcag gaccagcgcc
      601 ggcagtccgt aaaggaagct gcgcaacact tgcccggcgg aggggcgcac gccggttgcc
      661 atgtggccgc cggcccagaa gcgcgcgagc acggccagga tgacagccag gataatggct
      721 ggcatcagtc ccgcttcgaa caaggcggtg atggatgtgc tggtgaccgc cccgatgatg
      781 atgagcacga tcgagggggg gatggtttct gccatcgcgc cacagcttgc cgtgaccgcc
      841 aggatatgtc cgtcatcgat cttgtggcgt ttcatctctg gaacgacgat gggggccacc
      901 gccgccaggt ccgccgtctt ggcgcccgat atgcccgaca ccagcagaat gccgcctatc
      961 aacacataga acaggccgcc tttgacctgt ccaaacatgt ccgatagaaa gcgcatcatg
     1021 gccggggcaa ggcgcgtgac ggtcaacaga ccgcccaaca acacgaagag gggtacggca
     1081 agcaggatga tggaactcgt gccttgttcc acctggcttg ccatcacacc gagcggcgcg
     1141 ctaccggtga ccagcagata gaaaatgctg cagaagccaa aagcgaaggc gatgggtacg
     1201 ccgatcagca cggcggagaa caacagcacc acgaagacaa agacgacttc gaacaacgtg
     1261 ttgtcagaga gggccaggcc ggccagaaag gcggcgccaa tcaccaccac gacgctgatt
     1321 cccccgcgcc agaggtcgcc acggctgcgc cgaagcaggc cgcccaccag ataagccgcc
     1381 atcaaaacaa aacccacaac caggccggcg gcttcccagg cgctggaaat acccagttgc
     1441 gacaatgatg acagttgttc gatctgaagg aacgccaggc tcggttccac catcgccagc
     1501 acggccagca actgcatcag gtcggcggcg gcgcccagcc acccttgcca cgaagagggc
     1561 aagcggtggg tgaatgctgt catccgcatg tgctggtcgt cgcgcaccgc gaccacggcc
     1621 cccaccatgg tcagccagac gaaaacgagc cgggcgaact ccgtcgacca gatcaacgga
     1681 tggacaaaca cagtccgcgc gacgatgccg gcaaacagca cgagcacttc cgtcgccagc
     1741 aataccacgc cggccccatc caggcccgca tcggcgattt tgcgccatgg atagcccttg
     1801 gcgcggggcg tcacgccgcc cgtatcggat ttcatgattc ccgcgcctcc cgccacatcg
     1861 gttttcacca tttctgcctc ccttcgccac aacgacggtg tgcacaccac cggactccga
     1921 cggggcacac caccggactc cgatgaggca cacccctcct caaggcatga cgatcattcg
     1981 agttttattc cagcgccccg gaggtttttt cgagcagcgc ccaggctttc ccgccgaact
     2041 tgtccttcca gtgggggtag taatccgtct tgaccagggc gccccggaac gcgctcttgt
     2101 cgacgttcac aatgttcatg cccttggccg caagcttgcc tttcaacgaa tcgctcagtt
     2161 gcgcgatatc ggctcgttcc ttgcgtccgg catcggaaaa cgcttcggtg acgatgtcct
     2221 gccatttctt cggcagcctc gcgaaggccg gcttgttgcc gatgatgagg taaccgtccc
     2281 aggcatgctc cgtcatgctc aagtacttct ggacgtcgta cagcttggcg gtgtagatga
     2341 tcggcagcgg gttctcctgc ccgtccacca catgattctc cagggaggtg tagacctcgc
     2401 tgaaatccat cgaggtcggc gcggcatgga atgccgcgaa catcgacgac agaatcggcg
     2461 catcgggtac gcgaatcttg aaattgcgga gatccgcggg ggttttgacc ggcctcgttc
     2521 cagaggtgac gttgcggaac ccgttttccc acgacaggcc aatctgatgc atgccgtgct
     2581 ggtcgatttc attggcgata tggtggccaa gcgcgccatc gatcgccgac cacacatctt
     2641 tcggctcgag aaatgcgtac ccaatgttga cgatgcccgc ggcggggatc gtgcccgcca
     2701 ggacggagct ggccatgttc atgatctgaa cgccgcccaa ccgagtctgg gcgatgagct
     2761 gcttgtcggt acccagttgc gcggcaggat agagcttgat gtccagccca ccgtttgtct
     2821 cctttttgat ccgggcgatc gcctcggcgg cgcggacatt cacgggatgc gtcggttcct
     2881 ggcccgtggc cattttccag cggaactgcg ccgcgtcgct ttggcgggga agcatgccga
     2941 acgccgcggt ggcagcgata ccttgcaaca acgcccggcg actgatatgg ggctgcgtca
     3001 tgactttgtc tcctttctat ttaattatga aacgtggtgt cagatcccac gcccggcgct
     3061 attcccgtcg atgcgcgaac ctgcccagaa ttgagttttt cctgcgggcc cgggcccgcc
     3121 ctgtcttgcc gggcgtcaca accactccca gatccgccct gccttgccta acgtcacaac
     3181 cactccttga tccgccctgc cttgccaggc gtcacaacca ttccttgatc cgccctgcct
     3241 tgccaggcgt cacaaccatt ccttgatacg ccgggccatc gcttccgacg aaatgccgta
     3301 gcggtcatgc agggtgggca acgcgcccgc atccaggaag gcatcgggca gagcgatctg
     3361 tcggaacgcg ggtgccacgc ccgaacgcaa gagcagactg gccacggcct ctcccaggcc
     3421 accgatgacg gaatggttct cggccacgac aaccagtcgc ccgtgccgct tcacttcccg
     3481 caggatggtc tcttcgtcaa gaggcttgat cgtcggcacg tgcagcaccg cgacgtccac
     3541 actgtcgctg gccagcgcct tggccgcttc gagcgcgcgc atcgtcatca ggccgcacga
     3601 aatgaccaaa acatcccggc cgtcccggat caggctgcct ttgcccagtt caaaccggta
     3661 gtcgtattcg tccaggacca ggggcacctg gccgcgcagc aatcgcatgt acaccggccc
     3721 gtcgtgcgcc gcgatggcgg gaaccgcctg ttcgatgtcc agcgcgtcgc aagggtcgat
     3781 gatggtcagg cccggcacac cgcgcaggat cgagatgtcc tcggtggcct gatggctggg
     3841 gccatatccc gtggtcaacc ccggaagggc gcagcatatc ttgacgttga ggtgctcctc
     3901 ggcaatgatc tggtggatga aatcataggc gcgccgggtt gcgaacaccg cgtacgtggt
     3961 ggcaaacggg acgaacccct cttttgccat gccccccgcc gccgccatca gcaattgttc
     4021 ggccatgccc atttgaaaga accgctcggg gaaggccttc tggaagacat gcaagtccgt
     4081 gtatttggac agatccgccg tcatgccgac aatgtccgcg cgccgttcgg ccaattccac
     4141 cagcgcgtgt ccgaagggtg ccgcctgggt tcgttgtcct tcacccgcaa tcgaggcgat
     4201 catcgccgat gtcgtgagac gcggcttgtt ggctgttgtc tgactcattt ttccgctcca
     4261 tcgcgttgcg ccgcatccag aatatcgatc gccatccgcc actccggggc gtccactctg
     4321 atgaagtgat ttctttcacg ctgctccagg aacggaaccc cttttcctat caaggtatcg
     4381 aacaggatca cacgaggctg gtccccatcg tgatggcgtg cgcggtcgaa agcgtcgagc
     4441 acggcggcca gatcattccc atcgacacgc tggacgaacc acccgaaggc ggcccattta
     4501 tcgaccaacg gctcgaagcc gagcacatca cgggatggac catcggcctg ctggttgttg
     4561 atgtcgacca gggcgatcat gttcgcaagc cggtattggc tggcggacat ggcggcctcc
     4621 cacgtcgcgc cctcgtctaa ttcaccgtcg gacatcgaga caaccacgcg ggccggattc
     4681 tttttttgcc tcaggcccaa ggccatgcca acgccaatgc ccagcccctg ccccagggac
     4741 ccgccggaaa tctccattcc gggggtataa ctcgccattc cggacatggg caggcggctg
     4801 tcatcgcttc cgtaggtggc cagttcatcc agcggaatga cccccgcctc gatcagcgcg
     4861 gcatagtagg cgatggcata gtggccatgg gaaagcagga accggtcccg cccctcccag
     4921 tccggatcct ccggacgcag gttgagcgca tggcaatacg ccaccgcgag cacatcggcg
     4981 atgcccagtg cctggcctat atagccctgc ccttggacct cgcccatttg cagcgcgtag
     5041 cggcgaatgc ggtaggcgct gtcggcgagc cggcgcaatt cagccccggc acgaacttct
     5101 gtcactgcgg gtgtgttcat ttcattctcg tccatgagtc ggtgaatcca gtcagtggat
     5161 caacatgccg ccgttcacat cgatgaccgc tcccgtgatg taggaagaca gatccgatgc
     5221 caggaacagg taggcactgg ccacgtcctg cgcctccccc aggcggttca gtggtattcc
     5281 tttcaggatg tcgacccgca tggcgtcagt cagcttgccg ccggtgatgt ccgtctggat
     5341 cagccccggc gtgacgcagt tgacccggat gttgtccggc cccaattcac gggccatggc
     5401 cttggccagg cccagcacgc ccgccttggc cgctgaataa tgcgggccgc cgaaaatccc
     5461 ccctccccgc tgggcggaaa cagacgacat gcacgcgatg gagcccgatt tccgggcccg
     5521 catatgcgga atgaccgcct ggctcaaata cagaacgcca cccagattca cgtcaagaac
     5581 acgccgccag ttgtccggat cgatgtccat cgttttcaac ggctgcgtga tgcccgcgtt
     5641 gttgatcagc acgtcgatgc gcccgtacct ctccacgacg gccgcgacgg cctgctcgca
     5701 cgcttgcttg tcagcaacgt cacaagccag cccgacatga tggccggccc cgagagatgc
     5761 tgctgcctgc gctgcggatg atgcatccag gtcaagaatc gccacggtgg cgccatgggc
     5821 ggcgaacagc cgggccgtcg cgagccctat tcctcgaatg ctggccgccc ccgatatcgc
     5881 aacgatcttg tccttcaaaa gcatttcatg tctcctgaga atggttaggc ttgatagttg
     5941 aaatagtagg tggcgaaaac cctctggaca aacgatatgt ctgcaataaa taatgaattc
     6001 agttcaccaa tcatcctcca gggccatcat gttccagaac gtacctctgt cgatgttgcg
     6061 ggcgtttgaa gcgtccggac gcacgggctc attccgcaac gcggccgagg agctgtgctt
     6121 gtcgcccagc gcggtcagcc acgccattcg cggtcttgag gaacacctgg gtatccggct
     6181 gttcctgcgg agtacccgga agattcagct cactcccgaa ggcgaggcgc tgttcacgca
     6241 tatccggcgg gggttcgagg atctgcgcca cggcatggag ctggtgtcgg cgcgtgggcc
     6301 caccgtgctg cgcctgcact ccgcccccag tttcgcggcg cagtggctgg tcccccggtt
     6361 ggcgcgattt ttcaccgagc acccccgcat cgatctgaga ttggcggcca ataccgatta
     6421 cgcgcgcttc gaagcggacg agttcgacct ggacatcgtt tatggccagc ccaaatcaac
     6481 cggcaccgtt gtcgtccctt tgggcgaaga attgttgaca ccgctgtgct cccccgaaac
     6541 ggccacccgc atcaaatcgc ccaaagacct gattggattg cgattaattc agagcgaatc
     6601 caagcagatc cgttggccaa cctggctcga agcgaacaag cttcccatca acacgacgca
     6661 aggggccaga ttcgatcgca gctttctggc catcgccgca gcggcggatg gcctgggtgt
     6721 ggccttggag tcaacgcggc tggccgagcg ggaactgaag aacgggcgtc tcgtccggcc
     6781 cctggccggg caggaacacg aaatccggta caacgggcat tacctggcct attccagtgc
     6841 ggcgcatcaa cgccacacaa tcggcgtgtt cagagaatgg ttgctcgccg aactggcgga
     6901 cggatcattg gcgtcgcaat gaggcaacgt tcccacacgc gacgctgctt gcaatgattg
     6961 cactctctgg ctaaaatgca atcattgcaa gcacacagga gatcatcatg accgccaacc
     7021 tggtcgttcg caacatcgag cctgaagtcg tgcaggccct caagcaagcc gcagcccgcc
     7081 acgggcgtag cgccgaggcc gagcatcggg aaatcctgcg cgcgcaactg acccgccccg
     7141 cgcgccggtc tttcaaggac gtgctgggcg gcatgccgaa cgtcggcacc gacgaagact
     7201 tcaatttccg caacgcgcag ccttgatgta tctcatcgac accaatatcc tcagtgaggc
     7261 ccgcaagggc gtcagggcca acgccggggt gcgcgacttc tttgagaagc aagacagcca
     7321 gcacctgttt ctttccgtac tgaccgtcgg ggaaatccgc cggggactcg agcatattcg
     7381 gtgtcgtggc gatctcgccc aggccctccg cctggaaacg tggctggacg atctcgtcgt
     7441 cgaattcgac gatcacattc tcacgttcga catggcgtgc gcccaagtgt ggggcaaatt
     7501 gatgtcgccc aacgcccagc acgcgatcga caagcagatt gcggccaccg ccctgatctt
     7561 tgatctgacg gtggtcacgc gcaacctggc ggatttccaa tcgaccgggg ccaggctcat
     7621 caacccgttt cgatgatgtc gcgcttgacc aacccgattc gctcccccaa gccattccac
     7681 ggtcacgctt tcatcccaca ggcgtttgcg cgcgcgggct atacaatgct agcctcgtgc
     7741 tttttgaacg tgccgcctgg cggcatggcc atgagccatg tttacttgca ccccgataca
     7801 tcccgaagcg ccagatcggc accggcagga tactgtgtag cgcatgactg accaaccctc
     7861 ttcaacgact gatcccgtgc tgcgcctgga cggcgtggcg ctgggctatg gcgatgtcac
     7921 cgtgctcaac ggcatcgaca tggccatcgc gcccggccgc gtcgtggcgg tcatgggggg
     7981 gtcgggttcg ggcaagacca cgttgttgcg ggccgccaca gggcagatcc accccctggc
     8041 cggcactatc caggccttcg gccacgacgt cgccagcgcc gacaccaacg gcttgcagaa
     8101 gctgcgccag cgcatggggg tgttgttcca gcaaggcgcc ctgttcaccg acttgaacgt
     8161 cttcgagaac gtggcgtttc cgctgcgcga acaccggcac ttgccggagg acgacgtcac
     8221 ccgccaggtg ctggacaagc tcgatgcggt ggggttgcgc gccgccgctc acctgaatat
     8281 ttccgagatc tcgggcggca tggcgcgccg ggtggcgctg gcgcgcgcca tcgtgctgga
     8341 gcccgagctc attctgtacg acgagccctt cgcggggctg gacccgatct cgctgggcat
     8401 cacggcacaa ctcatccgcg gcatgaccga tcgcctgcac tgcgcgtccg tactcatcac
     8461 ccacgacgtg gccgaatcct tcgccatcgc cgaccatgtc tacatggtcg ggcaaggcaa
     8521 actgatcgcc agcggcacgc ccgacgaact gtcggcgtcc acagacccgt acgtgcggca
     8581 gttcctggac gccaagcccg acggcccgat cgccttcaac tatcccgaga cgtcccggtt
     8641 ccagcaatgg ctgtcgtccc gactcaaagg ccgcccatga cgctcgtcgc ccccatctcg
     8701 tcgatcgggc gtttcgtgcg cctgcgcatc accggcctgg gcgcgttcgc gcgctttttc
     8761 tggacgctgc tggcgcgcag cgcgctggtc gtgcgccgac ctggcctggt cagccagcag
     8821 gtgcatttca ttggcaacta ttcgctgctg atcatctcgg tgtcgggcct gttcgtgggg
     8881 ttcgtgctgg ggctgcaggg ctattacacg ctgaaccgct acggctcgga gcaggcgctg
     8941 ggcctgttgg tcgcgttgtc gctggtgcgc gagctggggc cggtgatcac cgcgctgctg
     9001 ttcgcggggc gcgccggcac ctcgctcacg gccgagatcg gcctgatgaa ggcgggcgag
     9061 caactggcgg ccatggaggt catggcggtc gacccattcc ggcgcgtgct ggtgccgcgt
     9121 ttctggggcg gcgtgatcgc catgcctatt cttgcggccg tgttctccat ggtggggatc
     9181 ctgggtggct ggttcgtggg ggtggtgctc atcggcatcg accccggcac gttctggtcg
     9241 cagatgcaaa gcggtgtcga cgtgttcaaa gacatcggca acggggtgtt caaaagcatt
     9301 gttttcgggg tggcggtgac cttgatcgcg ctgtaccagg gctggaccgc caaggccacg
     9361 cccgaggggg tggcgcgcgc cacgacccgc acggtggtca gcggcgccct ggtggtgctg
     9421 gggctggact tcctgctcac cgcgctgatg ttcagcaatt gaggccgggc tagttcacgc
     9481 atgacgcatg cacgccaccc acgcgacgcc tgtaacggat aaatcgacgg acaaacaatc
     9541 atgacgcgag aaaaaacaga tttctgggtg gggctgttcg tactgcttgg catcattgcc
     9601 ctggtgttcc tggccctgcg cgccggcaac ctgagctcgt tttccttcgc ccccacctac
     9661 caggtgacgg cgcgcttcga caacgtgggc agcctcaagg tgcgcgcgcc ggtcaagagc
     9721 agtggcgtgg tggtgggccg cgtagaaaac atcgccttcg acaacaagct gtaccaggcc
     9781 caggtcacgc tcgaactcca aaagcagtac cagttcgacg acgaaacctc ggcgtccatc
     9841 ctgacctcgg gcctgctggg agaacaatac gtggggttga cgcccggcgg cggcgacaag
     9901 atgctggccc agggcagccg catcaagtac acgcaaagcg ccatcgtgct ggaagacctg
     9961 atcagcaagt tcctgtacag ctcggccaac aaacagggta cggccagcgg ctcgtcgggt
    10021 ggcgggtcgg gtggcgggtc gggtggcgcg tcgtccggcg ggtcgctgtc ggcgccgggc
    10081 gcggcacccg cgaagcactg aggcatcgaa gccccgaaac gccgcctcgc cggggacgtc
    10141 acaacgctga aacagtgaat tcaacatact ggaacaatgg cttgatcctg tcgatatata
    10201 ctttcaaaac acggcaagcc gccgcaacca tcctataaat acaatgaacc ggccttcccc
    10261 cgctcttcca tttccgcccg ccgcccaagc gggccggcgt ctcgcgcgcc tggcccccat
    10321 gctggcgatc acgctggtcg ccgcggggtg cgcaaccgtc aagcacccca accccaacga
    10381 cccgtgggaa agctataacc gcggcatgta cgcgttcaac accaaggtcg acgacgtggt
    10441 cttcaagccc atcgccaagg gctacgaggc tgtcacgccc aagccggtgc gcacctgcgt
    10501 ccacaacatg ttcaacaacg tggaagacct ctggtcggcg ctcaacagct tcatccaggg
    10561 ccgtggggtg gatttcttca acaccctggg ccggttcctg gtcaacacca ccctggggct
    10621 gggtggttgc ttcgacgtaa acagccagtt gggcggccag cgcgtcgtca acgatttcgg
    10681 caccacgctg ggtgtctggg gcctgggcag cgggccatat gtcgtgctgc ccatcctggg
    10741 ccccagcacc gtgcgcgaca ctgccgcctg ggccaccacg gcggccgcca cgttctccga
    10801 gaactcccag ttcttctcgc cgctggcgcc catcatggcc atccgcaaca tccccgtgcg
    10861 caacggcgtg ctggcgttgc aggtcatcga tgcacgcgcc aatctgctcg acgccgaaaa
    10921 actcgtcaac cagatcgcgc tcgacaaata cagtttcatc cgcgatgcct tcctgcaacg
    10981 gcgccacgcc ctgctggaaa gcaagctgca tggcaagagc gccaacgcgc tgcccaacta
    11041 cggcgaccag gcgctgccga actattccga gcccgcgctg cccaagtacg acgatcccga
    11101 caaaccgggg tacacgggtg tcgtgccggt gaaggcccag ccagcggccg cgcccgccaa
    11161 gtccggcggg caagccgcca ccggtgcgcc ggccacatcc gcgaaacatt aaggagttct
    11221 ctatgtctct gtcttctctg gtgtgccgtt ggggtgattc gaacactcaa cttcggcgca
    11281 tgagttcgcc actcgccctt gcgcgccgcg tcatgctggc gggcctgctg gggctgggcc
    11341 tggtggccgt ggcgcaggct gacgaacccg tgaatgccaa tggcgcgccc aacgccttcg
    11401 tgcaggccgt gggcgacaac gcgctgcagg ccatcaagca ggcccatgtc aaacagggcg
    11461 acgtcgccaa gatcaaccag atcgtcgacc aatacgtgct gccttacgtc gacatgaaga
    11521 aaaccacgcg cctgtcggcc ggccgctact ggcgccaggc cacacccaag cagcaggccg
    11581 aactggtcaa tgccttcaag ggcacgctca tacgcaccta cagcggcgcc ttcaccaagg
    11641 tggaccccac ctcgaagttg gaagtcctgc cgttccgcgg cgaccccaaa gccgacgacc
    11701 tggccgtgcg ctcgacgctc acgcagacca actctccttc catcaatatc gactaccgcc
    11761 ttgaaaaaac cgcgcaaggc tggaaaatct acgacctgaa cgtggaaggc atctggctca
    11821 tccagaacta ccgcaaccag ttcgccgagc aaatcaccag caacggcata gacggtctca
    11881 tccaggcgct gaacaaccag aacaagtaag aacgtccgcc ggcagcctgg tcatcggcgg
    11941 cgccccgctc aataccttat ataatggtcg gtccggcctt tttcggccgg acccgctttc
    12001 actatgcccg ccctttccct ggaaaacatc tcgaagatct acccgcatcg ctccggcggc
    12061 tggcgaaaat ggctgtcgac gccggcttcc ggcgccggct tccaggcact ggatgatgtc
    12121 agcctgagca tcgattacgg tgagttcttc gggttgctgg gccccaacgg cgcgggcaag
    12181 accaccctca tttccatcct cgcgggcctg tcgcgcgcca cgaaggggcg tgccagcgtc
    12241 tgtggccacg atgtgctgac cgatttcaag cagtcgcgcc gggcgctggg cgtggtgcca
    12301 caggaaatcg tctacgaccc gttcttcacc gtgcgcgaaa cgctgcgcat ccagtccggc
    12361 tacttcggcc tgcgccgcaa tgacgactgg atcgacgaag tcctcagcaa cctgggcctg
    12421 gccgacaagg ccgacgccaa catgcgcgcg ctgtcggggg gcatgaagcg gcgcgtgctg
    12481 gtggcgcagg ccctggtgca ccggccaccg gtcatcgtgc tc
//
LOCUS       DASTNK010000008         7851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_933156, whole genome shotgun sequence.
ACCESSION   DASTNK010000008 DASTNK010000000
VERSION     DASTNK010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7851
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933156"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="VFR20_00530"
     CDS             complement(<1..163)
                     /locus_tag="VFR20_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0229029.1"
                     /translation="MSSNNDVVVVTGGASGIGLALAQGLLDTGRKVLALDLLEDRVAQ
                     ARESLRTQRA"
     gene            complement(326..724)
                     /locus_tag="VFR20_00535"
     CDS             complement(326..724)
                     /locus_tag="VFR20_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEU0229030.1"
                     /translation="MTTIEEGHTGRIFYVRIHPNEDLVQSLEKTFIEAGLSRAIVRSS
                     VGSLAHCSLECGADKQVDLPGPAVEILNMNGEISNDASGNPVSCITGMVMGMDGTFRA
                     GRFASGRNPACMTVEAVIEEWVVDHCLTAS"
     gene            complement(726..1190)
                     /locus_tag="VFR20_00540"
     CDS             complement(726..1190)
                     /locus_tag="VFR20_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229031.1"
                     /translation="MAGRNQKTYRIALRQGATLYACVAEAMASLNLKAAALNIEHVTF
                     SRISYFLITSSPDVEQVAHYTTALHLEEHAMLVGAGATVGANDRGDLAIHCHGLVVTD
                     SGDMVGGHFLTDQCVVAEDGVAYLSALGYMDLVIGVDPEIKTPVFQPKYCEA"
     gene            complement(1405..3036)
                     /locus_tag="VFR20_00545"
     CDS             complement(1405..3036)
                     /locus_tag="VFR20_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEU0229032.1"
                     /translation="MTRLSQQDPVGGLAPVSRRVMNLATFLSQAAARLPDCAALVIDG
                     QVTTWSELEQRVQALSRIFLEGGLQKGDRILIHSSNCRELLEVMLATFRIGAVWVPTN
                     FRITPDDVAYLAESAGVSAFICHTDFPAHAKLADGVANLKFVASVGPETGGVAGLDAQ
                     ISRRLEEGDAMMPHAQRVDYDDPCWFFFTSGTTGKPKAAVLTHGQMTFVLTNHLCDLM
                     PGTTDQDASLVVAPLSHGAGIHYLTQVVRGVVTVFSALPKFSAEAAWAAIEQYRVTNF
                     FTVPTILKFMVEHDSVDRYDHSSLRYIIYAGAPMYRADQKLALRKLGPVLVQYFGLGE
                     VTGNITVLPPHEHSPEDDGMPKLGSCGYARTGMELSIQDADGTPLEAGRQGEICVSGP
                     AVFAGYHNNPEANRKAFRDGWFRTGDLGYLDDQGYLYITGRESDMYISGGSNIYPREI
                     EEKILTYDAIKEVCVLGVPDDKWGEVGVMVGVLKDGAQWSEQAFQAWLDDNVARYKHP
                     RQVFIWPELPKSGYGKITKKIIYQELLQRGSLLKS"
     gene            complement(3033..4205)
                     /locus_tag="VFR20_00550"
     CDS             complement(3033..4205)
                     /locus_tag="VFR20_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEU0229033.1"
                     /translation="MAKKAVIVGWSHIPFGKLVEPDTETLMAQVSNSALEHAGITGKD
                     VDGIYVGVMNSGFQKQDFQGALVALANPDLAYVPATRLENACATGSAALYTAMDFIES
                     GRGRVALVVGAEKMTTRPNAEIGDILLNASYRKEEAEIKGGFAGVFGRIASEYFSRYG
                     DKSHELAMIAAKNHHNGVSNPYAQIRKDLGVEFCDQVSEKNPYVAEPLRRTDCSLVSD
                     GAAAIVLADEEYAAQLQRAVGFRARRHVNDIMPLSRRDPIAFEGASRAWRQALDAAGL
                     TLNDLSLVETHDCFTIAELIEYEAMGLAKRGEGYKVIREGITQKDGRLPVNPSGGLKS
                     KGHPVGATGVSMHVMACMQLTGTAGDMQIPNAQLAGVFNMGGAAVANYVSILERVK"
     gene            complement(4313..5134)
                     /locus_tag="VFR20_00555"
     CDS             complement(4313..5134)
                     /locus_tag="VFR20_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU0229034.1"
                     /translation="MEHSAAPASSARVSGTQAIHRAIKVLRCIARSRNEGVGLSGICR
                     QTGLIKSTVHRLTNALISEGLVQQDEASRRYFLGAECHALGLVASDRFGLHKFAARPV
                     ARLARETGDAAFFSVRQDAYSLCLMREEGDFPLKSRVLMAGDQHPLGVGSGSQAMLAA
                     LSDAEVEYCLEQNKADLARLYPRYTRELLLDLVRQTREKGYALNPGLVLAGSWGLGVV
                     VRGADGQVLGALSIATVESRMNPEREAQLYQWLRREADEIEKQVGADGPLRALVA"
     gene            5397..6413
                     /locus_tag="VFR20_00560"
     CDS             5397..6413
                     /locus_tag="VFR20_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEU0229035.1"
                     /translation="MRFSRRSFMLSAAGVAGAAAVGAPMSAFAAKTFRYKYANNLTPS
                     HPMNKRAKEMARAIEEETHGEFKLQIFPNSQLGSDTETLNQLRSGAVQFFTLSGLILQ
                     TLVPDAALSGIGFAFPDYASVWKAMDGKLGAFIRGKIKSHGLEVMDKIWDNGFREVTT
                     STHPIVTPADFHDLKIRVPVSPLWTSMFKALGAAPTSINFNEVYSALQTKVVDAQENP
                     LAIIQTAKLYEVQKYCSLTNHMWDGFWFLGNQDAWNKLPKDIQTVVAKHINEAGMLER
                     TDVAALNDELQKKLSAEGMKFNTPDPKPIRDALRKGGFYGEWKKKFGDDAWSVLEGSV
                     GTLT"
     gene            6424..>7851
                     /locus_tag="VFR20_00565"
     CDS             6424..>7851
                     /locus_tag="VFR20_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEU0229036.1"
                     /translation="MASQASAQLQTADGSRAPRPWISRLDAFLGHLIEIPVAILVLAE
                     IVILFAGIFARFVLNSPLIWSDELASLLFLWLAMLGAAVAFRRGEHMRMTAFVNKASP
                     TTQAFLNVFAIMVSLAFVLLVLIPSFEATIVQSVIHTAAMGISRSWRTAALPTGLSFM
                     AIFAILRLIEVSNWRVVLKALGLIVLIGAVLYALSPLFEDLGNYNLLIFFVGLVALGV
                     FTGVPIAFSFGIATYAYLLLSAQVPLTVLVGRMDEGMSHLILLAVPLFVFLGLLIEMT
                     SMAHKMVSFLASLLGHVRGGLSYVLVGAMYLVSGISGAKAADMAAIAPVLFPEMKKRG
                     ADEGELIALLSAAGAQTETVPPSLVLITIGSVTSVSITALFKGGLLPSAVLGLMLCMV
                     VWWRNRHQKLDHVERQPKLEILKSFIIAFPALTLPFVIRAAVVEGVATATEVSTIGIV
                     YSCFIGIFVYRCFNVKRLYPMLIDTA"
BASE COUNT         1382 a         2535 c         2384 g         1550 t
ORIGIN      
        1 ccgcgcgttg tgtgcgcagc gattcgcgcg cctgcgcgac gcggtcttcg agcagatcga
       61 gcgccagtac cttgcggccg gtgtcgagca acccctgggc cagcgccagg ccaatgcccg
      121 acgccccgcc ggtgaccacg accacgtcat tgttcgaact catcttgtct cctgtacggg
      181 tttacctgct ctctgaaaat cggcctgtgg ccttttcgac ttcttttcgc aatttgtctg
      241 tggcttcact catcgcccag ccagcggttc tttgcaggcg acggagcgtg acgcggcgcc
      301 acacggcgag tcacgctccg tcgcgtcacg acgcagtcaa acagtgatcc accacccatt
      361 cctcgatgac ggcctcgacg gtcatgcacg cggggttgcg ccccgacgcg aagcggcccg
      421 ctcggaacgt gccgtccata cccatcacca tgccggtgat gcaggacacg ggattgcccg
      481 acgcatcgtt gctgatttca ccgttcatgt tcaggatctc cacggcgggc ccgggcaggt
      541 cgacctgctt gtcggcgccg cattccagac tgcaatgcgc caggctgccc acgctgctgc
      601 gcacgatggc gcgcgacagg ccggcttcga tgaaagtctt ttccagactc tgcaccagat
      661 cttcgttggg gtggatacgg acgtagaaaa tccggccggt gtggccttct tcaatggtgg
      721 tcatgtcatg cctcacagta tttcggttgg aataccggcg tcttgatttc gggatcgacg
      781 ccaatcacca ggtccatgta gccgagcgcg ctcaggtacg ccacgccgtc ttcggcgacg
      841 acgcactggt cggtcaggaa atgccccccg accatatcgc ccgagtcggt gacgaccagg
      901 ccatgacagt ggatggccag atcgccgcgg tcgttggccc cgaccgtggc gcccgccccc
      961 accagcatgg cgtgctcttc gagatgcaag gcggtggtgt agtgggccac ctgctccaca
     1021 tcgggactgg aggtgatcag gaaataactg atacggctga acgtgacatg ttcgatgttc
     1081 aaggcggctg ccttgaggtt cagcgatgcc atggcctcgg ccacgcaggc atatagcgtg
     1141 gcgccctggc gcagggcgat tcggtacgtc ttttggttcc ggccagccat cacgtctcgc
     1201 cttgtgggcg cgacgggacc cggatgcttc aatagctgga ctgtcgatgc gggtgtcatc
     1261 atgatgtcct tgggttgata tggggatcca tccgcgctgg atggggtgtc gcgcgggtgg
     1321 atccgggtgt tcccttcggc tgacgcgtgg gcgagccaaa cggaacgcca gcatgctgtc
     1381 cgttggatac cgcgggacgg cgcactagct cttcagtaaa gaaccgcgtt gcaatagctc
     1441 ctggtagatg attttcttgg tgatcttgcc gtagccggac ttgggcagct ctggccagat
     1501 gaagacttgg cgcggatgct tgtagcgcgc cacgttgtca tcgagccagg cctggaacgc
     1561 ctgctcgctc cattgcgcgc catccttgag cactccgacc atgacgccga cctcgcccca
     1621 tttgtcatcg ggaacgccca gcacgcagac ttccttgatg gcatcgtagg tgaggatttt
     1681 ttcctcgatt tcgcgtggat agatgttgga gccgcccgaa atgtacatgt cggactcgcg
     1741 ccccgtgatg tacagatagc cttggtcgtc gagatagccc aggtcgccgg tgcggaacca
     1801 gccatcgcgg aaggccttgc ggttggcctc ggggttgttg tggtagccgg cgaacaccgc
     1861 cgggccgctc acgcagatct cgccctgccg gccggcctcg agcggtgtgc cgtcggcgtc
     1921 ctggatggag agctccatgc ccgtgcgggc gtagccacaa gagcccagct tgggcatgcc
     1981 atcgtcttca ggggagtgct cgtgtggcgg cagcacggtg atgttgccgg tgacctcgcc
     2041 caggccgaag tattgcacca gcaccgggcc cagcttgcgc agggcgagct tctggtcggc
     2101 ccggtacatg ggcgcgccgg cgtagatgat gtagcgcagc gacgagtgat cgtaccggtc
     2161 cacgctgtcg tgctcgacca tgaacttgag gatggtgggc accgtgaaaa aattggtgac
     2221 ccggtactgc tcgatggcgg cccaggcggc ttcggccgag aacttcggca gcgccgaaaa
     2281 cacggtgacc acgccgcgca cgacctgggt caggtaatga atgcccgcgc cgtgtgacag
     2341 gggcgcgacc accaggctgg cgtcctgatc ggtggtgcca ggcatcaggt cgcacagatg
     2401 gttggtcagg acgaacgtca tctgcccgtg cgtgagcacg gcggccttgg gtttgcccgt
     2461 ggtgccggac gtgaagaaga accagcatgg gtcgtcgtag tccacgcgct gagcgtgagg
     2521 catcatcgca tcgccctctt cgaggcgccg gctgatttgc gcatccaacc ccgccacgcc
     2581 cccggtttca ggacccacgc tggccacgaa cttgaggttc gccacgccgt cggccagctt
     2641 cgcgtgcgcc ggaaaatcgg tatggcaaat gaaagccgac acccccgcgc tttccgccag
     2701 gtaggccaca tcgtcagggg ttatgcggaa attcgtgggc acccacaccg cgccgatgcg
     2761 gaacgtggcc agcatgactt ccagcagttc gcggcaattc gacgaatgaa tgaggatgcg
     2821 atcgcccttc tgtaacccgc cttcgaggaa aatgcgggac agggcctgaa cccgttgctc
     2881 gagctcggac caggttgtga cctgtccgtc gatgaccagc gccgcgcagt ccggcaggcg
     2941 cgcggcggcc tggctcagga acgtggccag gttcatgacg cgccgggaca cgggcgccag
     3001 gccacccacc gggtcttgtt gcgacaaccg ggtcatttga cgcgctccag gatgctgacg
     3061 taattggcca cggccgcgcc acccatgttg aacacgccgg ccaattgcgc attggggatc
     3121 tgcatgtcgc cggcggtgcc ggtgagctgc atgcaggcca tcacatgcat ggataccccg
     3181 gtggcgccca cgggatgccc cttggacttc agcccgcccg acgggttcac cggcaggcgg
     3241 ccatccttct gggtgatgcc ttcgcgaatg accttgtagc cttcgccgcg cttggccagc
     3301 cccatggctt cgtattcgat cagttcggcg atggtgaagc aatcgtgggt ttcgaccagg
     3361 ctcaggtcgt tcagcgtgag gcccgcggcg tcgagcgcct gcctccaggc gcgcgaggcc
     3421 ccttcgaagg cgatggggtc gcggcggctg agcggcatga tgtcgttgac atggcgacgg
     3481 gcgcgaaacc caacggcgcg ctgcagttgc gcggcgtatt cctcgtcggc caggacgatg
     3541 gcggcggcgc cgtcggacac cagggagcaa tcggtgcggc gcaggggttc ggccacgtag
     3601 ggattctttt ccgacacctg gtcacagaac tccacgccca gatccttgcg gatctgcgca
     3661 taagggttgc tgaccccgtt gtgatggttc ttcgcggcga tcatcgccag ttcgtggctc
     3721 ttgtcgccgt agcgcgagaa atactcgctg gcgatgcggc cgaacacgcc ggcgaaacca
     3781 cccttgatct cggcctcttc cttgcggtag ctggcgttga gcaggatgtc gccgatttcg
     3841 gcgttggggc gcgtcgtcat tttctcggcg cccaccacca gcgcgacgcg gccgcggccc
     3901 gattcaatga agtccatggc cgtgtataac gcggccgacc ccgtggcaca cgcgttttca
     3961 aggcgcgtgg ccggcacata ggccaggtcg ggattggcca gcgccaccag ggcgccctgg
     4021 aaatcctgct tctggaatcc actgttcatc acgcccacgt agatgccatc gacatccttg
     4081 ccggtgatgc ccgcatgttc gagcgcgctg ttcgacacct gcgccatgag ggtttcggtg
     4141 tcgggttcga ccagtttgcc aaacgggatg tgcgaccatc ccacgatgac tgctttcttt
     4201 gccatgacta gtgtccttgc attgcattgc gattgagtga agattgccgc atcgggcggc
     4261 cctccgtgga agagttcatg tcgggaacgc gcaggccatc ggggatgcgc cctcacgcca
     4321 cgagtgcgcg cagcgggccg tcggccccaa cctgcttttc gatttcgtcg gcttcgcgtc
     4381 gcagccattg atacagctgc gcttcgcgtt cgggattcat gcggctttcg accgtggcga
     4441 tgctgagcgc acccaggacc tggccatcgg ccccgcgcac caccaccccc aggccccacg
     4501 aacccgccag caccagcccg gggttgagcg cgtaaccttt ttcccgcgtc tggcgcacca
     4561 ggtcgagcag gagttcacgt gtgtagcgcg gatacaggcg tgcgaggtcg gccttgttct
     4621 gctcgaggca atattccacc tcggcatcgc tgagcgcggc cagcatggcc tgactgccgc
     4681 tgcccacccc cagcgggtgt tggtctcccg ccatgagcac acgcgacttg agcgggaagt
     4741 cgccttcttc gcgcatgagg cacagcgaat aggcgtcctg ccgcacggaa aagaacgcgg
     4801 catcaccggt ttcacgagcc aggcgggcca cagggcgcgc agcgaacttg tgcagcccga
     4861 aacggtcgga cgccaccagg cccagcgcat ggcactccgc ccccaggaaa taacgccggg
     4921 aggcttcatc ttgctgcacc agcccttcgc tgatgagggc gttcgtgagc ctgtgcaccg
     4981 tggatttgat caggccagtc tggcggcaga tgccggacag cccaacccct tcattgcgac
     5041 tgcgggcgat gcagcgcaac accttgatgg cgcgatggat cgcctgcgtt cccgacacac
     5101 gggccgaaga cgccggtgcc gctgaatgtt ccatatcatg gacctattaa tttatttagt
     5161 ctgtctcgct agactcaatt ctacgtaaca ccctagtatt gtcaatgaat tttggaagaa
     5221 tttatttttt taatgttcca caagatagaa tttgaggata caataatttc gctattctta
     5281 atggtggacg gtccggctgt cacgcaacaa gtggttcgtg ctaccttctg atacgcgcgc
     5341 ttcccgcgct ccctctgcaa cataaataga tcattcatga tcaaggagac aaacagatga
     5401 ggttttcccg tcgctcattc atgctttcgg ccgctggcgt cgcaggcgcc gccgccgtgg
     5461 gcgcgcccat gtccgcattc gccgccaaaa cgttccgcta caagtacgcg aacaatctca
     5521 cgccgtcgca ccccatgaac aaacgggcca aggaaatggc ccgcgccatc gaagaagaaa
     5581 cccacggcga gttcaagctg cagatcttcc ccaacagcca gttgggctcc gacactgaaa
     5641 cgctcaatca gttgcgctcc ggcgccgtgc agttcttcac actgtccggg ctcattctgc
     5701 aaacactggt ccccgacgcg gccctcagcg gcatcggctt cgcgttcccc gactacgcca
     5761 gcgtctggaa agccatggat ggcaagctgg gcgcgttcat ccgcggcaag atcaagtcgc
     5821 atggcctgga ggtcatggac aagatctggg acaacggctt ccgcgaagtc accaccagca
     5881 cgcatcccat cgtcaccccc gccgacttcc acgacttgaa aatccgcgtg ccggtcagtc
     5941 cgctctggac atccatgttc aaggcgctgg gcgccgcgcc caccagcatc aacttcaacg
     6001 aagtctattc ggccctgcag accaaggtcg tcgacgccca ggaaaacccg ctggccatca
     6061 tccagacggc caagctctac gaagtccaga agtactgctc gctcaccaac cacatgtggg
     6121 acggcttctg gttcctgggc aaccaagacg cctggaacaa actgccgaaa gacattcaaa
     6181 ccgtggtggc caagcacatc aacgaagccg gcatgcttga acgcaccgac gtcgcggccc
     6241 tcaacgacga actccagaag aagctctcgg ccgaaggcat gaaattcaac acgcccgacc
     6301 ccaaacccat ccgcgacgcg ctgcgcaagg gggggttcta tggtgaatgg aagaagaaat
     6361 tcggcgacga cgcctggtcg gtcctggaag gctcggtcgg cacgctgacc tgataggcgc
     6421 accatggcat cgcaagcatc cgcgcagctt caaactgccg atggctcgcg tgcgccgcgg
     6481 ccatggatat cccggttgga tgcctttctc gggcatctga tcgagatacc cgtcgccatt
     6541 ctggtgctcg cggaaatcgt cattttgttc gccggcatat tcgcccgctt cgtcctcaat
     6601 tcgccgctga tctggtccga cgaactggcg tccctgctgt ttctctggct ggcgatgctg
     6661 ggtgccgccg tggcgttccg acgcggcgag cacatgcgca tgacggcatt cgtcaacaaa
     6721 gcgtcgccca ccacgcaggc gttcctaaac gtcttcgcca tcatggtgtc gctggcgttc
     6781 gtgctgctgg tgctgatccc cagcttcgaa gccaccatcg tccagtcggt catccatacc
     6841 gccgccatgg gaatcagccg ctcgtggcgc accgcggcgc tgcccaccgg cctgtcgttc
     6901 atggcgatct tcgccatcct gcgcctgatc gaagtctcga actggcgggt ggttctcaag
     6961 gcgctgggcc tgatcgtcct catcggcgcc gtgctctatg ccctttcccc gctcttcgaa
     7021 gacctgggca actacaacct gctcatcttc ttcgtgggcc tggtggcact gggggtcttc
     7081 accggcgtcc ccatcgcgtt ctcgttcggc atcgccacct acgcgtacct gctgctcagc
     7141 gcccaggtgc cgctcaccgt cctggttggt cgcatggacg aaggcatgtc gcacctgatc
     7201 ctgctggccg tgccgctctt cgtcttcctg ggcctgctca tcgaaatgac cagcatggcg
     7261 cacaagatgg tgtcgttcct ggccagcctg ctgggccacg tgcgcggcgg tctgtcctac
     7321 gtattggtcg gcgccatgta tctcgtatcg ggcatttctg gcgccaaggc ggccgacatg
     7381 gcggccatcg cgccggtgct gttccccgaa atgaaaaaac gcggcgccga cgagggcgag
     7441 ctcatcgcac tgctctcggc cgcgggcgcg caaacggaaa ccgtgccgcc cagcctggtg
     7501 ctcatcacca tcggctcggt caccagcgtg tccatcaccg cgttgttcaa aggcggcctg
     7561 ctgcccagcg cggtgctggg cctcatgctc tgcatggtgg tctggtggcg caaccgccac
     7621 cagaagctcg accacgtcga acgccaaccg aagctggaga tcctcaagag cttcatcatc
     7681 gccttccccg cgctgacgct gcctttcgtc atccgcgcgg cggtcgtcga gggggtggcc
     7741 acggccaccg aggtctccac catcggcatc gtgtattcgt gcttcatcgg catcttcgtg
     7801 taccggtgct tcaacgtgaa gcgcctgtac cccatgctca ttgacacggc c
//
LOCUS       DASTNK010000009        15068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1489570, whole genome shotgun sequence.
ACCESSION   DASTNK010000009 DASTNK010000000
VERSION     DASTNK010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15068
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489570"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VFR20_00570"
     CDS             complement(<1..776)
                     /locus_tag="VFR20_00570"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEU0229037.1"
                     /translation="MTDVGREAHVAPAQTQLSVSNYFDERIFALEQERIFQRSARYIG
                     HEKLVPETGDWRTLAHEDAGRVLVRGATGVELISNVCRHRQAIILGGEAGNVTSPHKA
                     CGNLGDTGGNIVCPVHRWTYNSQGKLLGAPQFPETPCLDLPRFTLKQCHGLLFEGPRN
                     PARDLAPLFDRPEFDFSDYVLDHVEIHRCHYNWKTFIEVYLEDYHVGPFHPGLGQFVT
                     CDDLAWEFAEHYSLQRVGIHNALAQPGTHTYKTWHDRLLN"
     gene            1064..1366
                     /locus_tag="VFR20_00575"
     CDS             1064..1366
                     /locus_tag="VFR20_00575"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU0229038.1"
                     /translation="MVEKTTGAPAKQADSGGTHGPQGADDLPQDFETALARLEGLVAQ
                     MEDGSLPLEQSLAAYQKGVELARICQRRLDAAEQQVRVLQEALLVPLNDATRADGD"
     gene            1369..2283
                     /locus_tag="VFR20_00580"
     CDS             1369..2283
                     /locus_tag="VFR20_00580"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEU0229039.1"
                     /translation="MPVRSVAFEAWLAARAQHVDAMLDQSLPEASTVPQTLHQAMRYA
                     TLGGGKRVRAALVYAAGEAALPAGTAVSAQEIALDHAAAAVEMVHAYSLVHDDLPCMD
                     DDALRRGRPTVHVRFNEATALLVGDALQPLAFDLLSQMPVAPALIVQATQILARAAGS
                     QGMAGGQAIDCESTGLTLTSEELQVMHAMKTGAMLEASVLLGGVVAGASSTVRRGLED
                     YAAAIGLAFQIVDDILDVTSDTATLGKTAGKDAVVDKPTYVSVLGLERSRELLESLRD
                     QAHDALRPLGSASRRLAEAADFIVGRDH"
     gene            2311..4197
                     /gene="dxs"
                     /locus_tag="VFR20_00585"
     CDS             2311..4197
                     /gene="dxs"
                     /locus_tag="VFR20_00585"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEU0229040.1"
                     /translation="MTTFTLDQIQSPADLRALDRRELPAVAEALREFVLQSVSRTGGH
                     LSSNLGTVELAIALHYVFNTPDDRLVWDVGHQSYPHKILTGRREAMARLRQRGGISGF
                     PKRSESEYDAFGTAHSSTSISAVLGMAVASRNLGMARQHIAVIGDGAMTAGMAFEALN
                     NAGVTPGVNVLVVLNDNDMSISPPVGALNRYLARLLSGGFYAAAKNVGRAVLQHVPPM
                     LELARRVEGHAKGLVVKPATLFEELGFNYVGPIDGHDLDALIPTLDNLRTLGGLQFLH
                     VITRKGQGYKLAEADPVLYHGPGKFDPEVGIQKPATPPKQTFTKVFGRWLCDMAQADD
                     KLVGITPAMREGSGLVEFEQRFPDRYFDVGIAEQHSVTFAAGLACEGLKPVVAIYSTF
                     LQRGYDQLIHDVALQNLDVTFALDRAGIVGADGATHAGNYDVAFLRCIPNMVVAAPSD
                     ENEARLLLSTCYRHPGPASVRYPRGAGCGAEVAGGLDAVEIGKGVVRLEGRRIAILGF
                     GTLVPAALDAGRRLGATVADMRFVKPLDEALIRSLVDTHEAFVTVEEASVMGGAGSAV
                     LEFLSAHGLHTPVLQLGLPDQFIDHGDQVALLRELGLDGDGIERSIRARFPDAARPPL
                     VAGI"
     gene            4273..5091
                     /gene="folE2"
                     /locus_tag="VFR20_00590"
     CDS             4273..5091
                     /gene="folE2"
                     /locus_tag="VFR20_00590"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HEU0229041.1"
                     /translation="MNSPIESSLALPDVAMPDVQSGFDTRRLAIQRVGVRGLRHPMLV
                     AQAGAGALPTVAQWELTVALPPEEKGTHMSRFVALLETYRPRPMTPELFCRMAQDMLP
                     LLNAERGEISAAFPYFLSKKAPVSGAESLLDYDIVWTARADGGAVEFELSMQVPLTSL
                     CPCSKAISDYGAHNQRSHVTVTVAFNDPGRVDMDALIRLTEQQASCELWGLLKRPDEK
                     FVTEQAYDNPKFVEDLVRDVAVHVRTVPGVLRFRVQAENFESIHNHSAYAVIEG"
     gene            5309..5686
                     /gene="rpsF"
                     /locus_tag="VFR20_00595"
     CDS             5309..5686
                     /gene="rpsF"
                     /locus_tag="VFR20_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU0229042.1"
                     /translation="MRHYEVVFIVHPDQSEQVPAMVERYQALVTGNGGQVHRLEDWGR
                     RQLAYPIQKLVKAHYVCMNIECGQETLDELEHSFRYNDAVLRHLVSKVKHAETAASIM
                     MKSVEREEARKATSESAGAAQAK"
     gene            5710..6003
                     /gene="priB"
                     /locus_tag="VFR20_00600"
     CDS             5710..6003
                     /gene="priB"
                     /locus_tag="VFR20_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEU0229043.1"
                     /translation="MNKLELDASVVEISPLRYTPAGVPALEMKLGHASEVAEAGGTRR
                     IELEVAAVALGDVALMLGSVAAGTLLSVEGFLAPARKGATRLVLHIQQANRRI"
     gene            6124..6396
                     /gene="rpsR"
                     /locus_tag="VFR20_00605"
     CDS             6124..6396
                     /gene="rpsR"
                     /locus_tag="VFR20_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU0229044.1"
                     /translation="MAFFKKNKEKRKFTQQNPLFKRRKFCRFTAAGVEQIDYKDLDTL
                     RDFIQENGKIIPARLTGTKAHYQRQLDTAIKRARFLALLPYTDNHK"
     gene            6412..6867
                     /gene="rplI"
                     /locus_tag="VFR20_00610"
     CDS             6412..6867
                     /gene="rplI"
                     /locus_tag="VFR20_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU0229045.1"
                     /translation="MQVILLEKVVKLGNLGEVVRVRDGYARNFLIPQKIARRATAAAL
                     KEFEERRAEHEKHQAEKLAAAQAVGEKLAGHTLTISQKAGVDGRLFGSVTNMDVAAAL
                     DAQGFGSFQKTQVRLPDGALKAVGEYPVQVALHADVVADITVVVQGEMA"
     gene            7010..8377
                     /locus_tag="VFR20_00615"
     CDS             7010..8377
                     /locus_tag="VFR20_00615"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU0229046.1"
                     /translation="MGGDPQLDSLRVPPHSVEAEQSVLGGLLLDNAAWDRITDVLTED
                     DFYRFDHRLIWQHVTRLIGLARPADVVTVHESLSSAGKAEDTGGLAYLNALAHNTPSA
                     ANIRRYAEIVRERAMLRKLITVADDIASTAFNPQGKEARQLLDEAESRVFQIAQEGSR
                     GASGFQEINPLLKSVVERIDELYHREGDNDVTGVPTGFADLDRMTSGLQPGDLVIVAG
                     RPSMGKTSFAMNIGEHVAINEGLPIAVFSMEMGAVQLAMRMVGSVGMLDQHRMRTGKL
                     NNDDWPRLTSAVQRVQEAQIYIDETPALNAMEVRARSRRLARQCGQLGLIIIDYMQLM
                     SASSSGENRATEISEISRSLKSLAKELNCPLMALSQLNRSLEQRPNKRPVMSDLRESG
                     AIEQDADLILFIYRDEVYNPDSPDKGMAEIIVGKQRNGPIGTVRLTFQGASTKFLNYA
                     GGSSY"
     gene            complement(8485..9069)
                     /locus_tag="VFR20_00620"
     CDS             complement(8485..9069)
                     /locus_tag="VFR20_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEU0229047.1"
                     /translation="MGKTRLEAFSDGVIAIIITIMVLELKVPHAPTFGALLDVAPVFL
                     SYVLSFVYVGVYWNNHHHMLHLADKIGGGVLWSNLHLLFWLSLLPFTTGWMGENHFAQ
                     ATLFLYGVNLLCCAIAYGILQRSIICLQGPDSTLARAIGRDIKGRISPVLYVLGVITS
                     FISPWLSGAFYVGTACLWLVPDRRVERELARPTH"
     gene            complement(9232..10308)
                     /locus_tag="VFR20_00625"
     CDS             complement(9232..10308)
                     /locus_tag="VFR20_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEU0229048.1"
                     /translation="MTFRFSPDPFLVKLLVVLVLASLLPAHGSGAIAFGWITDLAIAL
                     LFFLHGARLSREAVIAGMSRWRLHVAIFSATFLLFPILGVLIGPLVTPWVTRDLYLGI
                     LFLCCLPSTVQSSIAFTSIARGNVSAAVCSASASSLLGVFLTPLLVGVVMAGQATTHS
                     TVFSLDAIGKIMEQLLLPFVAGQLLRPWIGRWVTRHSQFLKLVDQGSILLVVYTAFSE
                     AVVGGLWANTPLSALLALLGILLALLFCVLALTHMLGRVLKFPLEDRITLLFCGSKKS
                     LSSGIPIANILFAGHAVGAIVLPLMIFHQIQLVVCAMIAGAYARRPVTQPKRGHPAHG
                     ESTPGGEPLAKRKRGRLRKGLEQR"
     gene            complement(10406..11419)
                     /gene="pdxA"
                     /locus_tag="VFR20_00630"
     CDS             complement(10406..11419)
                     /gene="pdxA"
                     /locus_tag="VFR20_00630"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEU0229049.1"
                     /translation="MTPPSMPARPVAITLGDAAGIGPEIIVKLYHAGLPFPGVVYGDA
                     GVLSTTIQRLGLQDRLTVRPVAGPGEGSADKGVIPVVNRWLPLPADLAPGKLDARAGR
                     AAYEYLCQAIDDASAGRLRAIVTAPLNKEAMGAGGIHYAGHTEILAERTHTQHYAMML
                     ANRELRVVLATIHAPLSAVPTLITTETELTVIRLAHEACRQAGIVAPRVAVAGLNPHA
                     GENGRFGQEDIQIIAPAIAQARTEGIEASGPWPGDTVFMRARNGEFDVVVAQYHDQGL
                     IPVKYLGVDHGVNVTIGLPFVRTSVDHGTAFDIAGQGVANPDSLREAYNLALDMTSAP
                     ARA"
     gene            complement(11462..13108)
                     /locus_tag="VFR20_00635"
     CDS             complement(11462..13108)
                     /locus_tag="VFR20_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU0229050.1"
                     /translation="MPLPSLPTRMGGSIQSAAVAEAMPEASAPLLEPIATPTPGNTEP
                     TGKTAEAPTVRPGKPTVTPRLAVVASPRRRKTRLASSRRKLFVLDTNVLLHDSSSLFK
                     FEEHDIFLPMIVLEELDHQKKGMSEVARNARQVSRYLDSLVAGSTSLEKGVELDSAGN
                     VEALGTLHFQTTAIHASLPIELPVGKADNAILTVVHALQGQYAKRDVILVSKDINMRL
                     KARALGLHAEDYYNDHVLDDSDLLYEGAMALPENFWTRHGKDVESWQQGGTTFYRVKG
                     PLCAEFIVNEFVYFEGDLPLYAQVREVNGKSAVLATLRDYSHGKNNVWGITARNREQN
                     FALNLLMNPECDFVSLLGQAGTGKTLLALAAGLTQTLETKRYNEIIITRATVPVGDDI
                     GFLPGTEEEKMLPWMGALEDNLEVLHLGANSADTASAQQPGNDWTKNSTMELIRSRIK
                     VKSLNFMRGRTFLNKYLIIDEAQNLTPKQMKTLVTRAGPGTKIICLGNIAQIDTPYLT
                     EGSSGLTYVVDRFKGWPHAGHVTLQRGERSRLSDYANEAL"
     gene            complement(13158..13625)
                     /locus_tag="VFR20_00640"
     CDS             complement(13158..13625)
                     /locus_tag="VFR20_00640"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEU0229051.1"
                     /translation="MSLAAIGKSLPAFSAQSTQGDISTASLKGNRAVLYFYPKDNTPG
                     CTTEAQNFRDRYQDYVSARCQIIGVSRDSLKSHLNFTEKQNLPFPLIADTEEALCQMF
                     GVIKLKNMYGRQVRGIERSTFLIDAHGILVQEWRGLRVPGHVDEVLQAVHGIA"
     gene            complement(13622..14074)
                     /locus_tag="VFR20_00645"
     CDS             complement(13622..14074)
                     /locus_tag="VFR20_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HEU0229052.1"
                     /translation="MLLQSESNPTLNTVTAYGQGYVEINAVHYEHAVYFAPDGAVTPL
                     EATCAADFTEALLKKIAGLNESGSDPLAFLDADAAPAKPDNAPEVILVGTGLTQRFLA
                     PDAVRTLTRLGIGVEAMTTQAAARTYNILMAEGRHVVVALLPHEETQQ"
     gene            14250..>15068
                     /locus_tag="VFR20_00650"
     CDS             14250..>15068
                     /locus_tag="VFR20_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0229053.1"
                     /translation="MTRFPRIERLPPYVFNITGELKMAARRRGEDIIDMSMGNPDGPT
                     PPHIVEKLVQVANRPDTHGYSVSKGIPRLRRAISGWYERRYGVSFNPDSEAIVTIGSK
                     EGLAHLMLATLDRGDTVLVPNPSYPIHIYGAVIAGANIRSVPMTPGLDFFVEIERAVR
                     ESIPKPKMMILGFPSNPTAQCVDLAFFERVVALAREHEILVVHDLAYADITFDGYVAP
                     SIMQVEGARDVAVEFFTMSKSYNMAGWRVGFMVGNAELVNALARIKSYHDYGTFT"
BASE COUNT         2733 a         4469 c         4973 g         2893 t
ORIGIN      
        1 taattgagca gccggtcgtg ccaggtcttg taggtgtggg tcccgggttg cgccagcgcg
       61 ttgtggatcc ccacgcgctg caggctgtag tgctcggcga attcccaggc caggtcatcg
      121 caggtgacga actgcccgag accggggtgg aacggcccca catgatagtc ctcgagatag
      181 acctcgatga aggttttcca gttgtagtgg catctgtgga tttccacatg gtccagcaca
      241 tagtccgaga aatcgaattc ggggcgatca aacaaggggg ccaagtcgcg cgctgggttg
      301 cgcgggcctt cgaacagcag cccatggcac tgcttgagcg tgaatcgtgg cagatcgagg
      361 cagggcgttt cggggaattg cggggcgccc agcagttttc cttgcgagtt gtaagtccag
      421 cggtggaccg ggcagacgat gttgcccccg gtgtcgccca ggttgccgca ggccttgtgc
      481 ggactggtga cattgccggc ttcgccaccc aggatgatgg cttggcggtg tcggcagacg
      541 ttcgagatca gttccacgcc cgtggctccg cgcaccagca cccgccccgc gtcttcatgg
      601 gcgagcgtgc gccagtcacc ggtttcgggt accagttttt cgtgcccgat atagcgggcg
      661 gaccgctgaa atatgcgttc ttgttctaga gcgaaaatac gctcgtcgaa atagttgctg
      721 acggacagtt gagtttgtgc cggcgcgacg tgcgcttccc gaccgacgtc ggtcatgggt
      781 ccccccgctt atggcagaac cggggcggct aggtgggcgg tcgtggcgcc ggcccggctc
      841 cggtgcgaag aaaaaaacgg cgtggccgat ggaaaatttg gagtcataaa ttgtacccga
      901 aattttttct tataaaacaa tgcgtattga caacgacggg ctatttcgcg cgtatccctt
      961 acaattgagg catgaatccg cgccgcgcga ccggacccgg gcgtgcgggt ggcttgcggc
     1021 ccgatcgggc cgcagcggcg ctttcccaaa actggacaca cttatggtgg aaaaaacgac
     1081 gggtgcgcct gcgaaacagg cagactctgg tggtacacac ggcccgcaag gtgccgatga
     1141 tctgccgcag gatttcgaaa ccgcgctggc gcggctcgag gggctggtgg cgcaaatgga
     1201 agacggctcg ctccccctcg agcagtcgct ggctgcctat cagaagggcg tcgagctggc
     1261 cagaatctgc cagaggcgcc tggatgccgc tgaacagcag gtacgggtat tgcaggaggc
     1321 gctgctggtt ccgctgaatg acgcgacccg cgccgacggg gattgatcat gccggtgcgg
     1381 tcggtcgcat tcgaagcgtg gctcgccgcg cgtgcgcagc atgtcgatgc catgctcgac
     1441 cagtctctgc ccgaagcctc gacggttccc caaactctgc accaggccat gcgctatgcg
     1501 acgcttggcg gtggaaagcg ggtgcgcgcg gcgttggtgt atgccgccgg ggaagcggct
     1561 cttcccgccg gcacagcggt ttcggcccag gagatcgcgc tcgaccatgc cgcggcggct
     1621 gtcgagatgg ttcacgccta ctctctggtg catgatgatc tgccctgcat ggacgacgat
     1681 gcgctgcgtc gaggccggcc cactgtccat gtgcgcttca atgaggccac ggcgctgctc
     1741 gtgggcgatg ccttgcagcc gctggccttc gatctgctat cgcaaatgcc cgtggcaccg
     1801 gcgctcatcg tgcaggccac gcagatactg gcacgagcgg ccggtagtca gggcatggcg
     1861 ggcgggcaag ccatcgactg cgagagcacc ggcctgacct tgaccagtga agagcttcag
     1921 gtcatgcacg ccatgaagac cggcgccatg ctcgaagcca gcgtgctgct gggtggcgtg
     1981 gtcgccggtg ccagttccac agtgcgccga gggctcgagg actacgcggc ggccattggc
     2041 ctggcgttcc agatcgtgga cgatatcctg gatgtcacgt cggacacggc tacgctgggc
     2101 aagacggcgg gtaaggatgc cgtggtggac aaacccacgt atgtatcggt gcttggtctg
     2161 gaacgttcac gcgaattgct ggaatcgctg cgcgaccagg cgcacgacgc gttgcgcccc
     2221 ctggggtccg ccagccgacg cttggccgag gcggccgatt tcatcgtcgg ccgcgatcat
     2281 tgatgtcatg tattgcaaga tgccactcgt atgactacct tcacgctcga tcagattcaa
     2341 tctcccgccg acttgcgcgc actcgaccga cgagaactgc cggcggtcgc ggaagcgttg
     2401 cgcgagtttg tcctgcagtc cgtgtcgcgc acgggagggc acttgtcgtc caacctgggt
     2461 acggtcgagc ttgcgatcgc gctgcattat gtgttcaata cgcccgatga ccgcctggtt
     2521 tgggatgtcg ggcatcagtc ctatccgcac aagatcctga ctggccggcg cgaggccatg
     2581 gcgcggctgc gccagcgcgg cggcatttcg ggttttccga aacgatccga atccgaatac
     2641 gacgcgttcg gcacggccca ttcctccacc tcgatttcgg ctgtgctggg catggccgtc
     2701 gcgtcgcgca atctggggat ggcacgacag cacatcgcgg tcatcggcga tggcgccatg
     2761 accgctggca tggccttcga ggccctgaac aacgcgggtg tgacgccagg cgtgaacgtg
     2821 ctggtggtgc tcaacgacaa cgacatgtct atctcccccc cggtcggtgc gctcaatcgc
     2881 tatctggccc ggctgctttc gggcggcttc tatgccgccg ccaagaatgt cggcagggcg
     2941 gtgttgcagc atgttccgcc catgctggaa ttggccaggc gggtcgaagg ccatgccaag
     3001 gggctcgtgg tcaagcccgc caccctgttc gaggagttgg gcttcaatta tgtggggccc
     3061 atcgatgggc acgacctcga tgcgctgatc ccgacgctcg ataatctgcg caccctgggc
     3121 ggactgcagt tcctgcacgt catcacgcgg aagggccagg gctacaagct ggccgaggcc
     3181 gatccggtgc tctaccacgg gcctggaaag ttcgaccccg aggtggggat ccagaagccc
     3241 gccacaccgc ccaagcagac cttcaccaag gtcttcggcc gttggctgtg cgacatggcc
     3301 caggccgacg acaagctggt gggcattacg ccagccatgc gcgaaggcag tggcctggtc
     3361 gaattcgaac agcggttccc cgacaggtat ttcgatgtgg gaatcgccga acagcattca
     3421 gtcaccttcg cggccgggtt ggcctgtgag ggtctcaagc cggtggtggc catctactcg
     3481 acattcttgc agcgtggata cgaccagctc atccatgacg tggcgctgca gaatctggac
     3541 gtcaccttcg cactggatcg ggctggcatc gtcggggccg acggagccac ccatgcgggc
     3601 aactacgatg tggcattcct gcgctgcatc cccaatatgg tggtcgctgc gccctcggac
     3661 gaaaacgaag ccaggttgct gctcagcacc tgctaccgtc atcccggccc cgcgtctgtg
     3721 cgctacccgc gcggcgccgg gtgcggcgcc gaggtggccg gtggactcga cgcggttgag
     3781 atcggtaaag gagtggtgcg gctcgaaggc cggcgcatcg ccatcctcgg gtttggcacg
     3841 ctggttccgg cggccctcga tgcggggcgc cgactcgggg ccaccgtggc cgatatgcgc
     3901 ttcgtcaagc cgttggacga agcgctcatc cgttctttgg tggacaccca cgaggccttc
     3961 gtcacggtcg aagaagcctc ggtaatgggc ggtgcaggca gcgcggtgct tgaattcctg
     4021 agtgcgcacg ggttgcacac accggtgttg caactggggt tgccagacca attcatcgat
     4081 catggggatc aggttgcgct gctacgggaa ctcgggctgg atggcgacgg catcgagcgt
     4141 tccatccggg cgcgtttccc cgacgcggcc cggccgccat tggttgccgg aatctgaacg
     4201 tgcaataatt atcatttact ttttcgtcaa gatgctccct tccacaaggg ggcgccttca
     4261 aggtttgctg gcatgaattc cccgattgaa tcctccctgg ctttgccgga tgtggccatg
     4321 ccagacgtgc agagtggttt cgacacgcgg cgtctggcta ttcaacgcgt gggcgtgcgt
     4381 ggcctgcgcc acccgatgct ggtggcgcag gccggcgcgg gcgcgttgcc cacggtggcg
     4441 cagtgggagc tgaccgttgc gctcccaccc gaggaaaagg gcacgcacat gtcgcgcttt
     4501 gtggcattgc tggaaaccta tcgcccccgt cccatgacgc cggaattgtt ttgccgcatg
     4561 gcacaggaca tgctgccgct gctcaacgcc gaacgtggcg agatttccgc ggcgtttccg
     4621 tatttcctgt cgaagaaggc gccggtttcc ggagctgaaa gcctgcttga ctacgacatc
     4681 gtctggactg cccgcgccga tggcggcgcg gtcgagttcg agctttccat gcaggttcca
     4741 ttgaccagcc tgtgtccgtg ctccaaggcg atttcggact acggcgcgca taaccagcgc
     4801 tcgcatgtca ccgtcaccgt ggctttcaac gatccggggc gggtggatat ggacgcgttg
     4861 atacgcctga ccgaacagca ggcgtcgtgc gagctttggg ggctgttgaa gcgcccggac
     4921 gaaaagttcg tcaccgagca ggcttatgac aaccccaagt tcgtcgaaga cctggtccgc
     4981 gatgtggcgg tgcacgtgcg caccgtgccc ggcgtcctgc ggttccgtgt ccaggccgag
     5041 aatttcgaat ccattcacaa ccactccgcc tacgcggtca tcgagggctg aggccgcgtc
     5101 ggccacgctg ttcatatgcc atgcgttttg ccaagcaggc ggaattcata gtagaatacg
     5161 cggtttcttg ctcgattcga acgtttttcc cgaccctggc gctttcgtgt caggaagggt
     5221 tggcatcgga cgggctgacc ctttcgccat ttttctttcg gctttgtggc cggtgatggg
     5281 cggaagacat cctcaaggag tgtggttcat gcgtcactac gaagtagtgt tcattgtgca
     5341 tcccgaccaa agcgagcagg tgcccgccat ggtcgagcgc tatcaggcgc tggtcaccgg
     5401 caacggcggc caggtgcatc gtctcgaaga ctggggccgc cggcaattgg cgtatcccat
     5461 ccagaaactc gtcaaagccc attatgtctg catgaatatc gagtgcggcc aggaaacgct
     5521 ggacgaactc gagcattctt tccgttacaa cgatgccgtg ttgcgccatc tcgtgtcgaa
     5581 ggtcaagcac gcggaaacgg cagcgtccat catgatgaaa tcggttgaac gcgaggaagc
     5641 ccgcaaggcg acttcggaat cggccggcgc ggcacaggca aagtaaacgt acggctgcca
     5701 ggccgcggcg tgaataaact cgaactggac gcttcggtgg ttgagatatc gccgctgcgc
     5761 tacaccccgg cgggggtgcc agccctggaa atgaaactgg ggcatgcatc cgaagtggcc
     5821 gaggcggggg gaacccgccg tatcgagctt gaggtcgccg cggtggcgct gggtgacgtg
     5881 gcgcttatgc tgggtagtgt ggccgctggc acgcttctgt cggtagaggg gtttctcgcc
     5941 cccgcccgca aaggcgcgac cagattggtt ttacatattc agcaggcgaa tcggcgcatc
     6001 tagccggcgg caccaggcgg gcctgggccg gaaacgcggc tccgaccgtg ggtttccgat
     6061 acggttgttt acgatcaatg cggattgtgg ggctcggccc cgaataaatt tacgaggcac
     6121 atcatggctt tcttcaagaa aaacaaggaa aaacgcaagt tcacccagca gaacccgctg
     6181 ttcaaacggc gcaagttctg ccgtttcacg gctgctggcg tcgagcagat cgactacaaa
     6241 gatctggata cgctgcgcga tttcatccag gaaaacggca agatcattcc cgcccggctt
     6301 accggcacca aggcgcatta ccagcgtcag ctcgatacgg ccatcaagcg cgcccgtttc
     6361 ctggcgctgc tgccgtacac cgacaaccac aaataatccg ggatcctcgt catgcaagtc
     6421 attttgctcg aaaaagtcgt caagctgggt aacctcggtg aagtcgtgcg ggttcgcgac
     6481 ggctacgccc gcaacttcct gattccccag aaaatcgcgc gccgcgccac ggccgccgct
     6541 ctgaaagagt ttgaagagcg ccgtgccgag cacgagaagc atcaggccga aaaactggcc
     6601 gcggcccagg ccgtgggcga gaagctcgcc ggtcatacgt tgacgatctc ccagaaggcc
     6661 ggcgtcgatg gccgtctgtt cggttccgtc accaacatgg acgtggcggc cgcgcttgac
     6721 gcgcagggct tcggcagctt ccagaaaacc caggttcgtc tgcccgacgg tgcattgaag
     6781 gccgtgggcg agtatcctgt gcaggttgcg ctgcacgccg acgtggtggc cgacatcacg
     6841 gtcgtggtgc agggcgaaat ggcctgaagt cgttcgcggg ctggcgctcg cgccgttgcg
     6901 gcgcggtgcg ccccccgcgt gccgtcctcc gcaaaaagcc ggcttcaccg gctttttgca
     6961 tttcaacggt ccactttgcg aggtgctggg attggaaagc gtagcgagat tgggcggcga
     7021 tccgcaactg gacagcttga gggtgccccc gcattcagtc gaggccgaac aatcggtgct
     7081 gggtgggctg ctgctcgaca atgccgcctg ggatcggatc accgatgtcc tgaccgagga
     7141 cgatttctat cggttcgacc accgcctgat ctggcagcac gtcacacggt tgatcggact
     7201 ggcccggccc gccgacgtgg tcaccgttca tgagtcactc tccagcgcgg gcaaggctga
     7261 agacactggc ggtctggcgt acctgaatgc gctggcgcac aacacgccct cggccgccaa
     7321 cattcgccgc tatgctgaaa tcgttcgcga gcgggcgatg ctgcgcaagc tcatcacggt
     7381 ggccgatgac atcgcgtcca ccgcgttcaa tccgcagggc aaagaggcgc gccaattgct
     7441 ggatgaggcc gagtcgcgcg tgttccagat cgcccaggaa ggctcccgcg gggccagcgg
     7501 ctttcaggaa atcaacccgt tgctcaagtc ggtggtcgag cgcatcgatg agctctacca
     7561 tcgcgagggt gacaacgacg tcacgggcgt gcccaccggc tttgccgacc tggaccgcat
     7621 gacgtccggc ctgcagcccg gagacctggt catcgtggcc gggcggccgt ccatgggcaa
     7681 gacctcgttc gccatgaaca tcggggagca cgtcgccatc aacgagggtc tccccatcgc
     7741 ggtgttttcc atggaaatgg gggctgtgca gctcgcgatg cgcatggtgg gctcggtggg
     7801 gatgcttgac cagcaccgga tgcgcactgg caagctgaac aacgatgact ggccgcgcct
     7861 gaccagcgcc gtgcaacggg tgcaggaagc gcaaatctat atcgacgaga cgccggcgct
     7921 caacgccatg gaagtgcgcg cccgatcgcg ccgtctggcg cgccagtgcg ggcagcttgg
     7981 cctgatcatc attgactata tgcaactgat gtcggcctcg tcatcgggcg agaaccgcgc
     8041 cacggaaatc tctgaaatca gccgttcgct gaagagcttg gccaaagaat tgaattgccc
     8101 gttgatggcc ttgtcgcagc tcaaccgcag ccttgaacaa cgccccaaca agcgccccgt
     8161 catgagcgac ctgcgcgagt cgggcgccat cgagcaggac gccgacttga tcctgttcat
     8221 ctaccgcgac gaagtctaca acccagactc ccccgacaaa ggcatggccg agatcatcgt
     8281 cggcaagcag cgtaatggcc ccatcggcac ggtgcgcctg acgttccagg gggccagtac
     8341 gaagttcctg aattatgcgg gcgggagttc gtactgagct gattcagttt ccgggtgtgc
     8401 ccgcggaatt gagcgtgtcg aatcggctca aacagtaccg cggctcatgt tccacgccat
     8461 gggcatttca aagccgaaga ggcattaatg cgtgggtcgc gccaactcgc gttccacacg
     8521 ccggtcgggg acaagccaaa ggcacgcggt gccgacatag aacgccccgg acagccacgg
     8581 cgagatgaac gacgtgatga cgcccagcac atacagcaca ggcgatattc tgcctttgat
     8641 atcgcgtccg atggcgcgcg ccagtgtcga atccggccct tgcaggcaaa tgatgctgcg
     8701 ttgcaatatt ccataggcga tggcgcagca taataggttg acgccgtaca gaaacagggt
     8761 ggcctgggcg aaatggttct cgcccatcca accggtcgtg aatggcagca gcgacagcca
     8821 gaacagcagg tgcagattgc tccacagcac gccaccgccg atcttgtcgg ccaagtgcag
     8881 catgtggtgg tggttgttcc agtagacccc cacgtagaca aagctcagaa cgtagctcag
     8941 gaagacgggg gcgacgtcga gcagcgcccc gaaggttggg gcgtggggca ctttcaattc
     9001 gagcaccatg atcgtgatga tgatggctat cacgccgtcg ctgaatgctt cgaggcgggt
     9061 ttttcccatg gtgggctttg cttgtgtatg aggagtttgg ctccacaatt gtacaggtgg
     9121 catccctctg taaacacggc atggcaggtt cgtgcatgca gaccagggcg cgggagccag
     9181 ccagacacgc agcgcgccgg gcgatccggc acgatgcgcg aggggcgtcg gtcagcgctg
     9241 ttccagcccc ttgcgcaaac gaccccgttt cctcttggca agtggttcgc cgccgggagt
     9301 tgattcccca tgtgcagggt gcccccgctt gggctgcgtg accggtcgtc gcgcataggc
     9361 acccgcgatc atggcgcaaa ccaccaattg aatctgatgg aagatcatca ggggcagcac
     9421 gatcgcgccg acggcgtggc cagcgaatag tatgttggcg atggggatgc cgctgctcag
     9481 gcttttcttc gatccgcaga acaacagcgt gatgcggtcc tccagcggga atttcaatac
     9541 ccggcccaac atgtgggtca gggccagcac gcagaacagc agagcgagca ggatacccag
     9601 cagcgccagc agggccgaca gaggggtgtt cgcccacagg ccgccgacca cggcctctga
     9661 aaaagccgtg tagaccacca gcaggatgga tccttggtcc acgagtttca ggaactggct
     9721 gtgccgggtt acccagcggc ctatccatgg acgcagaagc tggccggcga cgaacggcag
     9781 cagcagctgt tccatgattt ttccgatggc gtcgagcgag aacacggtgg aatgggtcgt
     9841 ggcctggccc gccatgacca ctccaaccag gagcggtgtc aggaagacac ccaacaggct
     9901 agaggccgag gcgctgcaca ccgccgccga cacattgccg cgcgcgatgg acgtgaatgc
     9961 gatggacgac tggacggtgg atggcaggca gcacaggaac agaatgccca ggtacagatc
    10021 ccgggtgacc cacggcgtga cgagcggccc gatcagcaca cccagaatcg gaaacagcag
    10081 gaacgtagcc gagaatatcg ccacgtgcag gcgccagcgc gacatccccg caatcacggc
    10141 ttcgcgtgaa aggcgggcgc cgtgcaggaa gaacagtagg gcgatggcga ggtcggtgat
    10201 ccagccgaat gcgatggcgc ccgagccgtg tgccggcagc aggctggcca ggaccaggac
    10261 aacaagaagt ttgaccagga aggggtcggg ggaaaagcga aaagtcatct gagatgaggt
    10321 aagcgctgga aaacgaaacc aaacgcatac ccgcatgggc cgccaggtgg ccacgggggt
    10381 gatgcgacgg gaatccgggg ggaagtcagg cgcgggctgg cgctgaagtc atgtcgagcg
    10441 ctagattata ggcttcccga agcgaatcgg ggttggccac accttgtccg gcgatgtcga
    10501 aggccgtgcc gtggtcaacg ctggtgcgca cgaacggcag accgatggtg acgttgacgc
    10561 cgtggtccac gcccaaatac ttgacgggta tcaggccctg atcgtgatac tgagcgacca
    10621 ccacgtcgaa ttccccgttc cgggcccgca tgaaaaccgt gtcgccgggc caggggccac
    10681 tggcctcgat gccctcggtt cgggcctgtg cgatggcggg cgcgatgatc tgaatgtctt
    10741 cctggccgaa acgcccgttc tcgccggcgt gggggttgag tcccgcgacc gccacgcgcg
    10801 gggctacgat gccggcttgg cggcatgcct cgtgggcgag gcggatcacg gtcagctcgg
    10861 tttcagtggt gatcagcgtc ggcaccgccg agagtggtgc gtggatggtc gccagcacaa
    10921 cgcgcagttc gcggtttgcg agcatcatgg cgtagtgctg cgtgtgcgtg cgttcggcga
    10981 ggatctcggt gtggccggcg taatggatgc ctccggctcc catggcttct ttgttcaggg
    11041 gggcggtgac gatcgcgcgc agccgccccg cgctggcgtc gtcgatggcc tggcacagat
    11101 attcgtaggc ggcccgtccg gcgcgtgcgt ccagcttgcc aggcgccagg tcggctggta
    11161 gaggaagcca tcggttcacg accggtatca cgcccttgtc cgctgaaccc tctccaggcc
    11221 cggcgacggg acggaccgtg agtcgatcct gcagcccaag acgttggatg gtggtcgaca
    11281 gtacgccggc gtcaccatag accacgcccg ggaacggcag cccggcgtga tagagcttga
    11341 caatgatttc ggggccgatg cccgccgcat cgcccaaggt gatggcgacg gggcgggcgg
    11401 gcatggaggg aggggtcatg agcattgagc ggtggcgccg gcgtgagtgg gcgccaccgt
    11461 gttacaaggc ctcgttggca tagtcggaca ggcgcgagcg ttcgccgcgc tgaagggtga
    11521 cgtgcccggc gtgcggccac cccttgaatc ggtccaccac ataggtgagc cccgaactgc
    11581 cttcggtcag gtagggggtg tcgatctggg cgatgtttcc caggcagatg atcttggtgc
    11641 cggggccggc gcgcgtcacc agggttttca tttgcttggg tgtcaggttc tgcgcttcgt
    11701 cgatgatgag gtatttgttc aggaacgtgc gcccgcgcat gaagttgagc gacttgacct
    11761 tgatgcggga gcgaatgagc tccatggtcg agttcttggt ccagtcgttg cccggttgct
    11821 gcgcggaggc ggtgtccgcg ctgttggcgc cgaggtgcag gacctcgaga ttgtcttcca
    11881 gggcgcccat ccagggcagc attttttctt cttccgtgcc gggcaggaac ccgatgtcgt
    11941 cccccaccgg caccgtggcc cgggtgatga tgatttcgtt gtagcgtttg gtctccagcg
    12001 tttgcgtcag gccggctgcc agcgccagca gtgtcttgcc ggtccccgcc tggccaagca
    12061 gcgagacgaa atcgcattca ggattcatga gcaggttcag ggcgaagttc tgttcccgat
    12121 tgcgcgccgt gatgccccag acgttgttct tgccgtggct gtagtcgcgc agcgtggcca
    12181 gtacggcgct cttgccgttg acctcgcgca cttgcgcgta caggggcagg tcaccctcga
    12241 aatagacgaa ttcgttgacg atgaattctg cgcacagcgg gcccttgacg cggtagaacg
    12301 tggtgccccc ttgttgccag gattcgacat ccttgccgtg gcgggtccag aagttctccg
    12361 gcagcgccat ggcgccctcg tacagcaggt cggagtcgtc gaggacatgg tcgttgtaat
    12421 agtcctcggc gtgcaggccc agtgcgcgcg ccttgagccg catgttgatg tccttggaca
    12481 ccaggatgac gtcgcgcttc gcgtattggc cctgcagggc gtgcacgacg gtcaggatgg
    12541 cgttgtccgc cttccccacg ggcagttcga tgggcagcga tgcgtggatg gcggtggtct
    12601 ggaagtgcag ggtgcccagc gcctcgacgt tcccggccga atcgagctcc accccttttt
    12661 cgaggctggt ggaacccgcg accaggctgt cgaggtagcg gctgacctgg cgcgcgttgc
    12721 gggccacttc cgacatgcct tttttctgat ggtcgagctc ttcgagcacg atcatgggca
    12781 ggaagatgtc gtgttcctcg aacttgaaca gggaactgga gtcgtgcagc agcacattcg
    12841 tgtcgagcac gaacagcttg cgccgggaag acgccaggcg cgtcttgcgg cgtcgcgggg
    12901 aggccacgac ggccaggcgc ggcgtgacgg ttggttttcc tgggcggacg gtgggggcct
    12961 cggccgtttt gcctgtgggc tcggtgtttc cgggcgttgg tgtcgcgatc ggttccagga
    13021 ggggggcgct tgcttcaggc atggcctccg ccacagcggc ggactggatg gatccgccca
    13081 ttcgggtggg gagcgaaggt aggggcatac gggaaggctc ctggcagagg gatggtctgg
    13141 gtcggggatg gtgtcggtca ggcgatgccg tgcaccgcct gaagcacctc gtcgacatgg
    13201 cccggaacac gcaagccacg ccattcctgg acgagaatgc catgggcatc gatgagaaac
    13261 gtgctgcgct cgatgccgcg gacttgccgc ccgtacatgt tcttgagctt gatgacgccg
    13321 aacatctggc agagggcttc ttcggtgtcg gcgatcagcg ggaagggcag attctgcttc
    13381 tcggtgaaat tcaggtggga tttcagggag tcgcgcgaga cgccgatgat ctggcagcgg
    13441 gccgagacgt aatcctgata acgatcgcgg aaattctggg cttcggtggt gcagccgggg
    13501 gtgttgtcct tggggtagaa atacagcacg gcccggttgc ctttgagcga cgcggtgctg
    13561 atgtcgccct gggtgctctg ggcggaaaag gcgggtaggg attttcctat ggctgccaga
    13621 ctcattgttg cgtttcctcg tgcgggagca gcgccacgac gacgtgacgc ccctcggcca
    13681 tcaagatgtt gtaggtgcgt gccgccgcct gggtggtcat ggcctcgacg ccgatgccca
    13741 ggcgcgtcag ggttcggacg gcgtcggggg caaggaaccg ttgcgtcagt ccggtgccga
    13801 ccagaatgac ttccggcgcg ttgtcgggct tggctggcgc cgcgtcggca tcgaggaacg
    13861 ccaggggatc gctcccgctt tcgttcagac cggcgatctt cttgagcaat gcctcggtga
    13921 agtcggccgc acaggtggct tcaaggggag tgaccgcgcc atctggagcg aaatagacag
    13981 catgttcgta gtgcacggca ttgatttcga cgtagccctg gccgtaggcc gtgacggtgt
    14041 tgagcgtggg atttgattcg gattgcagca gcacatgagc ccccaatagt cctctgcatg
    14101 atagcgcaat ccgttttgta aaatgatggc gaaagaaatt tttcccatcg tataaactac
    14161 tgaattccgc gtctttccgg cattccggcg ccgccgcgcg gcggcggcgc acgtttcctc
    14221 cgttccgtcc atatctggga tgtatgtcca tgacccgctt tcctcgcata gagcgcttgc
    14281 ctccctatgt cttcaacatc actggtgagc tcaagatggc ggcgcgccgc cggggcgagg
    14341 acatcatcga catgtcgatg ggcaaccccg acggccccac ccccccccac atcgtcgaaa
    14401 aactggtcca ggtggccaac cgccccgata cccatggcta ttcggtgtcc aagggcattc
    14461 cgcgcttgcg ccgggcgatt tcgggctggt acgagcgacg ctacggtgtt tcgttcaatc
    14521 cggactccga ggccattgtc accattggtt ccaaagaagg cttggcgcac ttgatgctgg
    14581 ctacgctgga tcgcggcgac acggtgctgg tgcccaatcc aagctatccc atccacattt
    14641 acggcgcggt catcgccggc gccaacattc gctcggtgcc catgaccccg gggctcgatt
    14701 tcttcgttga aatcgagcgt gccgtgcgcg aaagcattcc caagcccaag atgatgattc
    14761 tgggtttccc cagcaatccc acggcgcagt gcgtcgacct ggccttcttc gagcgcgtgg
    14821 tggcgttggc ccgcgagcac gaaatcctgg tcgtgcacga cctggcctac gccgacatca
    14881 ccttcgacgg ctatgtggcg ccgtccatca tgcaggtcga gggcgctcgc gacgtggcgg
    14941 tggagttctt caccatgagc aagagctaca acatggccgg atggcgtgtc gggttcatgg
    15001 tgggcaatgc ggaactggtc aacgcgctgg cgcgcatcaa gagctaccac gattacggga
    15061 cgttcacg
//
LOCUS       DASTNK010000010         9892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_748989, whole genome shotgun sequence.
ACCESSION   DASTNK010000010 DASTNK010000000
VERSION     DASTNK010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9892
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748989"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1745)
                     /gene="nuoL"
                     /locus_tag="VFR20_00655"
     CDS             complement(<1..1745)
                     /gene="nuoL"
                     /locus_tag="VFR20_00655"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEU0229054.1"
                     /translation="MSSTTLYLVIALAPLVGAILAGFFGTGFLGSQLGRKATHRITIL
                     CVFISFLGSLAVLSQVLNGHTFDGTIYTWTLIGTSKLNIGFLIDPLSALMMAVVTFVS
                     LMVHIYTIGYMADDPGYNRFFAYISLFTFSMLMLVMSNNMLQLFFGWEAVGLVSYLLI
                     GFWYTKPTAIFANLKAFLLNRVGDFGFILGIGLLFGYAGSMHYADVFGQADKLAGVAF
                     PGTDWHMITVACLCLFVGAMGKSAQVPLHAWLPDSMEGPTPISALIHAATMVTAGIFM
                     VARFSPVFEHSDTALSVMLVIGAIGALFMGILGIIQNDIKRVIAYSTLSQLGYMTVAL
                     GASAYSVAIFHLMTHAFFKALLFLGAGSVILGMHHDQDIRNMGGLGKYMPITAITFLL
                     ATLALVGTPFFAGFYSKEHIVEAVGAANIWGAHFAYYCTLGGVFVTSLYSFRVYFLVF
                     HGKERFHDPQYGEHAAHAHDDHDNHDAHDTHGHGGHPKESPWVVTLPLVLLAIPSVVA
                     GALFIDPLLFGGYFKHAIEVLPIHPAMADITEHWHGWLEYALEGFTSTAFLLLVAGLV
                     VAWYCYLINPRVPAA"
     gene            complement(1762..2070)
                     /gene="nuoK"
                     /locus_tag="VFR20_00660"
     CDS             complement(1762..2070)
                     /gene="nuoK"
                     /locus_tag="VFR20_00660"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEU0229055.1"
                     /translation="MTITLAHYLVLGAILFAIGIFGFFLNRRNLIILLMSIELILLAA
                     NMNFVAFSSWSGDTAGQVFVFFILTVAAAEAAIGLAILVLLFRNLNTINVEELDHLKG
                     "
     gene            complement(2067..2723)
                     /locus_tag="VFR20_00665"
     CDS             complement(2067..2723)
                     /locus_tag="VFR20_00665"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEU0229056.1"
                     /translation="MIFSTVLFYLFSLVLVIASIRVITASNPVTAVLHLILAFFTASM
                     LWMLLDAEFLALLLVLVYVGAVMVLFLFVVMMLDFRHGTPREGLKTYLPLGLIVGIIM
                     VLEMAFVVVKSWTGAGAPKSLPANYDNTHALGVAMYTHYVFAVEVGAVVLLVGMVAAI
                     ALTLRHRRDVKYQRPGDQVRVHKRDRLRIVSIESQTEPRADSTPAASMEAGLAKGESK
                     "
     gene            complement(2742..3230)
                     /gene="nuoI"
                     /locus_tag="VFR20_00670"
     CDS             complement(2742..3230)
                     /gene="nuoI"
                     /locus_tag="VFR20_00670"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEU0229057.1"
                     /translation="MEAIKDFFSSLMLTEMFEGLRLTGKYFFKRKITLRYPNEKTPKS
                     PRFRGLHALRRYPNGEERCIACKLCEAVCPALAITIESAEREDGSRRTTRYDIDLYKC
                     IFCGFCEESCPVDSIVETHISEYHGEERGDLYFTKDMLLAVGDRYEAEIAERRAADAP
                     YR"
     gene            complement(3250..4323)
                     /gene="nuoH"
                     /locus_tag="VFR20_00675"
     CDS             complement(3250..4323)
                     /gene="nuoH"
                     /locus_tag="VFR20_00675"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009456680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEU0229058.1"
                     /translation="MNWLTAFEGAGSGLLGPTLWLVVWTLIKIVIIAVPIILCVAYLT
                     YWERKMIGAMHVRLGPTRVGYKGLLQPFADVFKLLLKEIIQPAKSNKVLYLLAPVVTL
                     MPALAAWAVIPFGPEAVLSNVNAGLLYVMAITSLGVYGVIVAGWASNSKYAFLGALRA
                     SAQMISYELPVGFVMITVLLVSGTLNMNGIVMGQGRGWFASHGLTFLSWNWLPLLPLF
                     VIYVISAVAETNRLPFDVVEGESEIVAGHMVEYSGMSFALFFLAEYANMILLSALASV
                     MFLGGWMSPLDFAPFTWIPGWIWLGIKTFIVVSMFIWFRATFPRYRYDQIMRLGWKIF
                     IPLTGVWLLVVAIWMQTPWNIWH"
     gene            complement(4320..6650)
                     /gene="nuoG"
                     /locus_tag="VFR20_00680"
     CDS             complement(4320..6650)
                     /gene="nuoG"
                     /locus_tag="VFR20_00680"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEU0229059.1"
                     /translation="MVELTIDGVKVEAPEGSMVIQAAHSAGVYVPHFCYHKKLTVAAN
                     CRMCLVEVEKAPKAVPACATPVTNGMVVYTHSQKAVDAQKSVMEFLLINHPLDCPVCD
                     QGGECQLQDLAVGYGASASRYQEPKRVVVSKEMGPLVSAEEMQRCIHCTRCVRFGREI
                     GGIMEVGMINRGEHAEITSFVGRAVESELSGNMIDVCPVGALTSKPFRFNARTWELAR
                     RRSVSPHDSLGANLVVQVKDHVVRRVVPFDNEDVNECWISDRDRFAYEGLYVEDRLTH
                     PMIKEGGQWREASWSDALQAVVKGIGRVREQHGDDQLGAIASPIATTEELALLARLTR
                     ALGSQNVDFRLRQTDAGFDQALAGVPWLGLPVAELGQLDRVLVVGSFLRKDHPLMAQR
                     LRQAVKHGTQVSFIGPVADDPLFTSVHGRMTVAPSVLANAVAEVLVAAAQASGQSVPE
                     ALADITPSAEAQRIAASLASGQKTAVLMGNVAIASPQASLIAANASALATLAGARFGF
                     LTAGGNTVGGYLAGATPREGGLSAEQMLAQPLKAYLVVHAEPALDADNGARAVATLRS
                     AEFAVALTSYRSAAEDWAHVMLPISPFTETSGTFVNAEGRVQSFKGTVEAYGDTRPAW
                     KVLRVLGNLFQLPGFEDETSESVRDGVLVEGVQGRLSNEIRVAVGASRPHDGLERVAD
                     WPIYRTDPIVRRSAPLQESDASRLPEARMAADTLRKLGVASGARVRVRSGQGEVVLTA
                     LNDDTVAPGCVRVSAGFAETAALGGAFEHITVEQAA"
     gene            complement(6666..8030)
                     /gene="nuoF"
                     /locus_tag="VFR20_00685"
     CDS             complement(6666..8030)
                     /gene="nuoF"
                     /locus_tag="VFR20_00685"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEU0229060.1"
                     /translation="MTAPLLSQFSQGLDGAPGGDLSNSMMFHGRHINPLIFDGLDGDN
                     WRLEDYVKRGGYEALRKILSTGMKPEDVIAEVKASSLRGRGGAGFPTGLKWSFMPRTL
                     PGQKYLVCNSDEGEPGTFKDRDILRFNPHSVIEGMAIGAYAMGISVGYNYIHGEIFEV
                     YERFEEALEEARAAGLLGDNILGSGFNFQLHGFHGYGAYICGEETAMLESLEGKKGQP
                     RFKPPFPAGFGMYGKPTTINNTETFAAVPWIFRNSAREFLELGLPNAGGTKIFSVTGD
                     VRLPGNYEVPLGTPFSELLELAGGMREGRKLKAVIPGGSSAPVLPADIMMGLTMDYDA
                     IAKAGSMLGSGAVIVMDDTRCMVKSLLRLSYFYFEESCGQCTPCREGTGWLYRMVNRI
                     EHGEGRPEDLEVLDSVAHNIMGRTICAFGDAAAMPVRSFIKHFRDEFAHHIEHKCCVV
                     PKYL"
     gene            complement(8027..8518)
                     /gene="nuoE"
                     /locus_tag="VFR20_00690"
     CDS             complement(8027..8518)
                     /gene="nuoE"
                     /locus_tag="VFR20_00690"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEU0229061.1"
                     /translation="MLLSEQAYRKIDRELTKFPAEKKRSAILASLTIAQDEKGWVSPE
                     VIEDVANYLGQPPIAVQEVASFYNMLDTKPVGRYKLSVCTNLPCALRGSEEALAHVRK
                     KLGVDLGETTADGLFTVIEAECMGACGDAPLVLVNNKQMCVRMSEDKLDAMIDLIQQE
                     GKA"
     gene            complement(8555..9811)
                     /locus_tag="VFR20_00695"
     CDS             complement(8555..9811)
                     /locus_tag="VFR20_00695"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEU0229062.1"
                     /translation="MAEIKNYTLNFGPQHPAAHGVLRLVLELDGEVVQRADPHIGLLH
                     RATEKLAEHKTFLQALPYMDRLDYVSMMCNKHAYVMAIEKLMGVQVPLRAQYIRVMFD
                     EITRILNHLMFLGSHALDVGAMGVFLYAFREREDLMDCYEAVSGARMHAAYYRPGGVY
                     RDLPDSMPQYGETSKYRGAKELRVMNDARSGTLLDFIEDFTNRFPKCVDEYETLLTEN
                     RIWKQRLVGIGVVSPDRAKALGFTGPMLRGSGVEWDLRKMQPYEVYDRVQFDIPVGTN
                     GDCYDRYLVRVTEMRESNRIIAQCVNWLRNNPGPVIVDNHKVAPPSREHMKTGMEDLI
                     HHFKLFSEGMHVPEGEVYASIEHPKGEFGIYMVSDGANKPYRLKIRAPGFVHLQSLDE
                     MARGHMIADVVTLIGTQDIVFGEIDR"
     gene            complement(9815..>9892)
                     /locus_tag="VFR20_00700"
     CDS             complement(9815..>9892)
                     /locus_tag="VFR20_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEU0229063.1"
                     /translation="YQPVTIEPREVTPRVVREENYGIGR"
BASE COUNT         1899 a         3162 c         3122 g         1709 t
ORIGIN      
        1 atcgccgccg gcacacgcgg attgatcagg tagcagtacc aggccaccac cagacccgcg
       61 accagcagca ggaacgccgt ggacgtgaac ccctccagcg cgtattcgag ccagccatgc
      121 cagtgttcgg tgatgtcggc catcgccgga tggatgggca gcacttcgat ggcgtgcttg
      181 aagtagccac cgaaaagcag cgggtcgatg aagagggccc ccgcgaccac cgacggaatg
      241 gccagcagca ccaagggcag cgtgaccacc catggcgact ccttcgggtg cccgccgtgg
      301 ccatgcgtat cgtgcgcgtc atggttgtcg tggtcgtcat gtgcatgcgc cgcgtgctcg
      361 ccgtactggg gatcgtggaa tcgttccttg ccgtggaaca ccaggaaata cacccggaac
      421 gaatacagcg aggtcacgaa cacgccaccc agggtgcagt aatacgcgaa gtgcgcgccc
      481 cagatgttgg cggcgccaac cgcctcgacg atgtgttcct tggaatagaa tccggcgaag
      541 aacggcgtgc ccaccagcgc cagcgtggcc agcaggaacg tgatggcggt gatgggcatg
      601 tacttgccca gcccgcccat gttgcggatg tcctggtcgt gatgcatgcc caggatgacc
      661 gagcccgcgc ccaggaacag cagcgccttg aagaacgcgt gcgtcatcag gtggaagatc
      721 gcgaccgaat acgccgaggc cccgagcgcg accgtcatgt agcccagctg cgagagcgtg
      781 gaatacgcga tcacgcgctt gatatcgttc tggatgatgc ccaggatgcc catgaacagc
      841 gcgccgatgg ccccgatgac cagcatgacc gacagcgccg tgtcggaatg ctcgaacacg
      901 ggcgagaacc gcgccaccat gaagatgccg gccgtcacca tcgtggcggc gtggatcagg
      961 gccgaaatgg gggtcgggcc ttccatggaa tcaggcagcc aggcatgcag cggcacctgt
     1021 gccgatttac ccatggcgcc cacgaacagg cacaggcagg cgaccgtgat catgtgccag
     1081 tcggtgcccg ggaacgccac gccggccagc ttgtcggcct ggccgaacac atcggcgtag
     1141 tgcatgctgc cggcgtagcc gaacagcagg ccgatgccca ggatgaagcc gaagtcaccc
     1201 acgcggttga gcaggaacgc cttcaagttg gcgaagatgg ccgtgggctt cgtgtaccag
     1261 aaaccgatca gtaggtacga caccaggccc accgcctccc agccgaagaa caactggagc
     1321 atgttgttgg acatgaccag catcagcatc gagaacgtga acagcgagat gtacgcgaag
     1381 aagcggttgt agccaggatc gtcggccatg tagccgatgg tgtagatgtg caccatgagc
     1441 gacacgaacg tgaccaccgc catcatcagc gccgacagcg ggtcgatgag gaaaccgatg
     1501 ttgagcttgg aggtgccgat cagtgtccag gtgtagatgg tgccgtcaaa cgtgtggccg
     1561 ttcaacacct gggacaggac cgcgagcgac cccaggaagg aaatgaacac gcacaggatg
     1621 gtgatgcggt gcgtagcttt gcggcccagt tggctgccca ggaacccggt gccgaaaaag
     1681 cccgcgagaa tggccccgac caggggggcc agcgcgatga cgagataaag cgtagtggaa
     1741 ctcatgtctg tgccgccccc atcaaccctt gagatgatca agttcttcaa cattgatcgt
     1801 gttcagattg cggaacagca ataccaggat cgccagcccg atggccgcct ccgccgccgc
     1861 gacggtgaga atgaagaaaa cgaacacctg ccccgccgtg tcgcccgacc agctcgagaa
     1921 cgccacgaaa ttcatgttgg ccgccagcag gatcagctcg atggacatga gcagaatgat
     1981 caggttgcgg cgattcagga agaagccgaa gatgccgatg gcgaacagga tggcgcccag
     2041 caccaggtag tgcgctagtg tgatggtcat ttgctttccc ctttggccag gcccgcttcc
     2101 atagatgccg ccggcgttga atcggcccga ggctcggtct gcgattcgat gctgactatg
     2161 cgcagacggt cgcgcttgtg cacccggacc tgatcgcccg gacgctggta tttgacgtca
     2221 cgccgatggc gcagcgtcag cgcgatggcc gccaccatgc ccaccagaag caccaccgcg
     2281 ccgacttcca cggcgaacac gtagtgcgtg tacatggcca cacccagcgc atgggtgttg
     2341 tcgtaattgg ccggcagtga tttcggcgcg ccggcgcccg tccaggactt gaccaccacg
     2401 aacgccatct ccagcaccat gatgatgccg acgatcagcc ccagcggcag ataggtcttg
     2461 agcccttcgc gcggggtgcc atggcggaag tccagcatca tgacgacgaa caggaacagc
     2521 accatgacgg cgcccacgta gaccagcacc agcagcagcg ccaggaattc cgcatcgagc
     2581 agcatccaaa gcatggacgc ggtgaagaac gccaggatca ggtgcagcac ggccgtgacc
     2641 gggttgcttg ccgtgatgac gcggatcgac gcgatcacca gcaccagcga gaacaggtag
     2701 aacaggacag ttgagaaaat catgggtatc gggtcctggc ttcagcggta aggggcgtcg
     2761 gcggcgcggc gttcggcgat ctcggcttca tagcggtcgc ccacggccag cagcatgtcc
     2821 ttggtgaagt acaggtctcc ccgctcttcc ccgtggtatt cggaaatatg cgtctcgacg
     2881 atggagtcca ccgggcagct ttcctcgcag aagccgcaga agatgcactt gtataggtcg
     2941 atgtcgtaac gcgtggtgcg gcgcgaaccg tcttcccgct cggccgactc gatggtgatg
     3001 gccagcgccg ggcagaccgc ctcgcacaat ttacaggcga tgcagcgctc ttcgccattg
     3061 gggtagcggc gcaacgcgtg caggccgcgg aagcgcggtg acttgggcgt cttctcgttc
     3121 ggatagcgca gcgtgatctt gcgcttgaag aaatacttgc ccgtgaggcg caggccctcg
     3181 aacatctcgg tcagcatcag gctgctgaaa aaatctttga ttgcttccat gattggtctc
     3241 cgggcgcgat cagtgccaga tattccaggg cgtttgcatc cagatcgcca ccaccagcag
     3301 ccacacgccg gtgaggggga tgaaaatctt ccagcccaga cgcatgatct ggtcgtaacg
     3361 ataacggggg aacgtggcgc ggaaccagat gaacatcgac accacgatga acgtcttgat
     3421 gcccagccag atccagcctg gaatccaggt gaacggcgcg aaatcgagcg gcgacatcca
     3481 accgcccagg aacatgacag acgccagcgc cgacaacagg atcatgttgg cgtattcggc
     3541 caggaagaac agggcgaaac tcatgcccga gtattccacc atgtgcccgg cgacgatttc
     3601 cgattcgccc tcgaccacgt cgaacggcag gcggttggtc tcggccacgg ccgaaatcac
     3661 gtagatgacg aacaacggca gcagcggcag ccagttccac gacaggaagg tcaagccgtg
     3721 cgacgcgaac cagccacggc cctgccccat cacgatgccg ttcatgttga gcgtgcccga
     3781 caccagcagc accgtgatca tgacgaagcc caccggcagt tcgtacgaga tcatttgcgc
     3841 cgacgcgcgc agcgcgccca ggaaggcgta cttggaattc gacgcccagc ccgcgacgat
     3901 caccccgtac acgcccagcg aggtgatggc catcacgtac agcagcccgg cgttgacgtt
     3961 ggacagcacg gcttcggggc cgaacggaat caccgcccac gccgccagcg cgggcatcag
     4021 cgtgacgacc ggcgccagca ggtacagcac cttgttggat ttcgccggct ggatgatttc
     4081 cttgagcagc agcttgaaca cgtcggcgaa cggctgcagc aggcccttgt agcccacccg
     4141 cgtcggcccc aggcgcacgt gcatggcacc gatcatcttg cgttcccagt acgtgaggta
     4201 ggccacgcac agaatgatgg gaacggcgat gatgacgatc ttgatgaggg tccagaccac
     4261 cagccagagc gtgggcccca gcaggcccga gccggcccct tcgaacgctg tcaaccaatt
     4321 catgccgctt gctccacggt gatgtgttca aacgcgccac ccagcgccgc ggtttcggcg
     4381 aagccggccg aaacgcgcac gcaacccggc gccacggtgt cgtcgttcag tgccgtgagg
     4441 acgacctcgc cctgcccgga acgcacccgg acccttgcgc cggaggccac gcccagcttg
     4501 cgcagggtgt cggccgccat gcgggcctcg ggcaggcggg acgcgtcgga ttcctgcagc
     4561 ggcgccgagc gccgcacgat gggatcggtg cggtaaatgg gccaatccgc cacgcgctcg
     4621 agcccgtcgt gcggccggct cgcgcccacc gcgacgcgaa tctcgttcga gagccggccc
     4681 tgcacgcctt cgaccagaac gccgtcgcgc acggattcgg aggtttcatc ctcgaagccc
     4741 ggcaactgga acagattgcc cagcacgcgc agcaccttcc acgcggggcg cgtgtcgcca
     4801 taggcttcca ccgtgccctt gaagctttgc acccgccctt ccgcattgac gaacgtgccg
     4861 gaggtttccg tgaacggcga gatcggcagc atgacgtgcg cccagtcctc ggccgccgac
     4921 cggtaggacg tcagcgccac ggcgaactca gccgatcgca gcgtcgcgac ggcacgggca
     4981 ccattgtcag cgtccagggc gggctcggca tgcaccacca gataggcctt gagcggttgc
     5041 gccagcatct gttcggcgct caggccacct tcgcgcggcg tcgcgccggc caggtagccg
     5101 cccaccgtgt taccaccggc ggtgaggaac ccgaaccgcg cacccgccag cgtggccagc
     5161 gccgaagcgt tggcggcgat gagcgaggcc tgcggcgacg caatggccac gttgcccatc
     5221 agcaccgctg tcttctggcc ggaggccagg ctggccgcga tgcgctgggc ctcggcggag
     5281 ggggtgatat ccgcgagggc ttccggcacg gactggcccg acgcctgcgc ggccgcgacc
     5341 agcacctcgg ccactgcatt ggccaggacg ctgggcgcca cggtcatgcg accatgtacc
     5401 gacgtgaaca gcggatcgtc ggcgaccggg ccgatgaacg acacctgggt accgtgcttg
     5461 acagcttggc gcaagcgttg cgccatgaga ggatggtctt tgcgcaggaa cgagcccacc
     5521 accagcacgc gatcgagctg gccgagctcg gccaccggca gacccagcca gggcacaccg
     5581 gccagcgcct ggtcaaaccc ggcgtcggtc tggcgcaggc ggaaatccac gttctgcgag
     5641 cccagggcgc gcgtcaggcg cgccagcagg gcgagttcct cggtcgtggc gatggggctg
     5701 gcgatggcgc caagctggtc gtcgccgtgc tgttcacgca cgcggccgat ccccttgacc
     5761 accgcctgca gcgcgtcgct ccatgacgcc tcgcgccact gcccgccttc cttgatcatg
     5821 ggatgggtca ggcggtcttc gacatacagc ccttcatagg cgaagcgatc gcggtcgctg
     5881 atccagcatt cgttgacgtc ctcgttgtcg aacggcacca cgcggcgcac gacatggtcc
     5941 ttgacctgca cgacgaggtt cgcgcccagg ctgtcatgcg ggctgaccga cctgcgccgc
     6001 gccagttccc aggtgcgggc gttgaaacgg aacggcttgg acgtgagcgc gcccaccgga
     6061 cagacgtcga tcatgttgcc ggagagctcg gattcgaccg cgcgacccac gaatgaggtg
     6121 atctcggcgt gttcaccgcg gttgatcatg cccacttcca tgatgccgcc gatctcgcgc
     6181 ccgaaccgca cgcaacgcgt gcaatggatg cagcgctgca tttcctcggc cgacaccagc
     6241 ggccccattt ccttggacac caccacgcgc ttgggttctt gatagcgcga ggccgaggcg
     6301 ccatagccca cggccagatc ctgcaactgg cattcgccac cctggtcgca cacggggcaa
     6361 tcgagcggat ggttgatcag caggaattcc atgacgctct tctgcgcgtc cacggccttc
     6421 tgtgaatggg tgtagaccac catgccgttg gtgacgggcg tggcgcaggc cggcacggcc
     6481 ttgggcgcct tttccacttc gaccaggcac atgcggcagt tcgccgccac ggtgagcttc
     6541 ttgtgataac agaaatgcgg cacgtacacc ccggcgctgt gcgcggcctg tatgaccatg
     6601 ctgccttcgg gggcttcgac cttgacgccg tcgatggtga gttcaaccat gagctgtatc
     6661 ctgacctaca aatatttggg caccacgcag cacttgtgct cgatgtggtg tgcgaactcg
     6721 tcgcggaaat gcttgatgaa gctgcgcacc ggcatggccg ccgcgtcgcc gaaggcgcag
     6781 atggtgcgtc ccatgatgtt gtgggccacg gaatcgagca cttccaggtc ttcaggccgg
     6841 ccttcaccgt gctcgatgcg gttgaccatg cggtagagcc agcccgtgcc ttcgcggcaa
     6901 ggcgtgcatt gcccgcagct ttcctcgaaa tagaagtacg acaggcgcag cagcgacttc
     6961 accatgcagc gcgtgtcgtc catgacgatg accgcgcccg aacccagcat ggagcccgcc
     7021 ttggcgatgg cgtcatagtc catggtgagg cccatcatga tgtcggcggg cagcaccggc
     7081 gcgctggacc cgccggggat caccgccttg agcttgcgcc cctcgcgcat gccgccggcc
     7141 agctccagca attcggagaa cggcgtgccc aatgggactt cgtagttgcc gggaaggcgg
     7201 acgtcccccg tgaccgagaa gatcttggtg ccgcccgcgt tgggcaggcc aagctcaagg
     7261 aattccctgg cgctgttgcg gaaaatccag ggcaccgccg cgaaggtttc ggtgttgttg
     7321 atggtggtgg gcttgccgta catgccgaag ccggcgggaa acggcggctt gaagcgcggc
     7381 tggcctttct tgccctccag cgattccaac atggcggttt cctcgccgca gatgtaggcg
     7441 ccgtagccat ggaacccgtg cagttggaaa ttgaagcccg acccaagaat gttgtcgccc
     7501 agcaaacccg ccgcgcgggc ttcctcgagc gcttcctcga agcgttcgta cacctcgaag
     7561 atttcgccgt ggatgtagtt gtagcccacg ctgatgccca tggcgtaggc accgatggcc
     7621 atgccctcga tcacgctgtg ggggttgaaa cgcaggatgt cgcgatcctt gaatgtgccc
     7681 ggctcgcctt cgtcggagtt gcacaccagg tacttctgcc ccggcagcgt gcgcggcatg
     7741 aagctccatt tcagcccggt ggggaaaccc gcgccgccac gcccgcgcag gctggacgcc
     7801 ttgacctcgg cgatgacgtc ttcgggcttc atgcctgtgg acaggatctt gcgcagggcc
     7861 tcgtagccgc cacgcttgac gtaatcttcc agacgccagt tgtcgccatc gagcccatcg
     7921 aaaatcaacg gattgatatg gcgcccatgg aacatcatgc tgttggacag atcccccccc
     7981 ggggcgccgt ccaggccttg agaaaactgg ctgagcagtg gcgcggtcat gccttgccct
     8041 cctgctggat caggtcgatc atggcgtcga gcttgtcttc cgacatgcgc acgcacatct
     8101 gcttgttgtt gaccagcacc agcggcgcat cgccgcaggc gcccatgcac tcggcctcga
     8161 tcaccgtgaa caggccatcg gccgtggttt cacccaggtc gacgccgagc ttcttccgca
     8221 catgggccag cgcctcttcg gatccacgca gggcacaggg cagattggtg cacaccgaca
     8281 gcttgtagcg gcccaccggc ttggtgtcga gcatgttgta gaacgaggcg acttcctgca
     8341 ccgcgatggg cggctggccc aggtaattgg ccacgtcctc gatgacctcg ggcgagaccc
     8401 agcctttttc atcctgggcg atggtcagcg acgccaggat ggcggaccgc ttcttttcgg
     8461 ccggaaactt ggtgagctcc cggtcgatct ttcgataggc ttgttcagaa agcagcataa
     8521 ttcgaacctg gtagtatctt cgatgcccgg ccggtcaacg gtcgatctca ccgaacacga
     8581 tgtcttgcgt gccgatcagg gtgaccacgt cggcgatcat gtggccgcgc gccatttcgt
     8641 ccagcgactg caagtgcacg aagcccggcg cgcggatctt cagacggtac ggtttgttgg
     8701 cgccgtcgga caccatataa atgccaaact cgcccttggg atgctcgatg ctggcgtaga
     8761 cctcgccctc gggcacgtgc ataccctcgg agaacagctt gaagtggtgg atgaggtctt
     8821 ccatgccggt cttcatgtgt tcgcgcgagg gcggcgccac cttgtggttg tcgacgatga
     8881 ccggacccgg gttgttgcgc agccagttca cgcactgggc gatgatgcga ttgctttcac
     8941 gcatctcggt gacgcgcacc agatagcggt cgtagcagtc accattggtg cccaccggaa
     9001 tgtcgaactg cacacggtcg tagacttcgt agggctgcat cttgcgcaga tcccattcca
     9061 cgcccgagcc gcgcagcatg gggccggtga agcccagggc cttggcgcgg tcgggcgaga
     9121 ccacgcctat gcccaccagg cgctgcttcc agatacggtt ttccgtcagc agggtttcgt
     9181 attcgtccac acacttgggg aagcggttgg tgaagtcttc gatgaaatcc agcagcgtgc
     9241 ccgaacgcgc atcgttcatg acgcgcaatt ccttggcgcc gcgatacttg ctggtttcgc
     9301 catactgcgg catggaatcc ggcaggtcgc ggtacacgcc gcccggccgg taataggccg
     9361 cgtgcatgcg cgcgcccgac accgcctcgt agcagtccat caggtcttca cgttcacgga
     9421 acgcgtacag gaacacgccc atggcgccca cgtcgagcgc gtgcgagccc aggaacatca
     9481 ggtggttcaa tatgcgcgtg atctcatcga acatgacgcg gatgtactgc gcgcgcagcg
     9541 gcacctggac gcccatcagc ttctcgatgg ccatcacata ggcgtgcttg ttgcacatca
     9601 tggaaacata atccaggcgg tccatgtagg gcagcgcctg gaggaatgtc ttgtgctcgg
     9661 ccagcttttc ggtagcccgg tgcagcaggc cgatgtgcgg atcggcgcgt tgcacgactt
     9721 cgccgtcgag ctccagcacc aggcgcagca caccgtgcgc cgccgggtgc tgcggcccga
     9781 agttcagggt gtaattcttg atttcggcca tgctctagcg ccctattccg tagttctctt
     9841 cacgcaccac gcgaggcgtg acttcacgcg gctcgatggt gacgggctgg ta
//
LOCUS       DASTNK010000011        19238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_933942, whole genome shotgun sequence.
ACCESSION   DASTNK010000011 DASTNK010000000
VERSION     DASTNK010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 19238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19238
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933942"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1059
                     /gene="ugpA"
                     /locus_tag="VFR20_00705"
     CDS             178..1059
                     /gene="ugpA"
                     /locus_tag="VFR20_00705"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HEU0229064.1"
                     /translation="MEKRVVFSHKTLPYLLLIPQLAVTVVFFFLPAGQAMWQSFRLED
                     AFGLSSQFVGWQNFIDLFSNPDYINSFKVTTEFSLLVAISGLAIALLLAVMANRVVKG
                     AGLYKTLLIWPYAVAPAVAGVLWLFLFSPAVGILAVYLAEMGFHWNPRLNGHDAMTLV
                     VLTSVWKQISYNFLFFLAGLQSIPRSLIEAAAIDGASPFRRFWTIVFPLLSPTTFFLL
                     IINVIYSFFDTFAIIDVMTQGGPGNSTSILVYQVYNAGFKGMDIGSSAAQSVILMIIV
                     VALTVIQFRYIDRKVNY"
     gene            1081..1923
                     /gene="ugpE"
                     /locus_tag="VFR20_00710"
     CDS             1081..1923
                     /gene="ugpE"
                     /locus_tag="VFR20_00710"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HEU0229065.1"
                     /translation="MIERRPYLDIFSHVILLLGVAIVAFPLYVTFVASTQTAQEVARA
                     PMSLLPGSHFLQNYASVLFGQGSGSVPVARMMWVSTVMALVIAIGKIVISMLSAFAVV
                     YFRFRFRMFFFWMIFVTLMLPVEVRIAPTYKVVADLGMLNTYAGLTLPLIASATATFL
                     FRQFFLTIPDELIEAARIDGAGPMRFFRDILFPLSKTSIAALFVIQFIYGWNQYLWPL
                     IITTHEDMYPIVIGIQRMIAGGDSVTSWNLVMATALLAMLPPAAVVILMQKWFVKGLV
                     DAEK"
     gene            1974..3059
                     /locus_tag="VFR20_00715"
     CDS             1974..3059
                     /locus_tag="VFR20_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HEU0229066.1"
                     /translation="MATLNFRNVTKTYAGGVAVIHGISMDVTDGEFIVIVGPSGCGKS
                     TLMRMVAGLETVTGGDIVIGDKVVNTLEPAERDIAMVFQNYALYPHMSVFQNMAYGLK
                     IRKLDKEDIKRRVDSAAEILEIGHLLERRPGQLSGGQRQRVAMGRAIVREPKVFLFDE
                     PLSNLDAKLRVQMRLEIQKLHRRLKTTSLYVTHDQVEAMTLAQRMVVMNKGVAEQIGT
                     PIEVFENPASTFVASFIGSPPMNLIPVEISPERTLRGVDGTPFDLEPAQVPADLAGRK
                     AILGIRPEHMRLRKPGIPVDIEMVEVLGSEQLIHGRIGDTQLVVRCPVSLTRDQPVRV
                     GDAFPVGSDGVHPLHWFDAGTGKRIVE"
     gene            complement(3194..4315)
                     /locus_tag="VFR20_00720"
     CDS             complement(3194..4315)
                     /locus_tag="VFR20_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU0229067.1"
                     /translation="MKIRFTPIVAALGITVGMLVSSGANAAGTIKIAVAGPFTGPLTQ
                     YGGMVKQGVDTEVEKVNAAGGVLGKKLEVVVMDDGCEPKQAPVVANRVVNDDIHYVVG
                     HVCSGATIAATDIYNNNGVLMISPSATSPLVTDGKHYDMIFRTIGRDDQQGPVAGKFI
                     ADKIKPKRVAVLHDKQSYGQGIATAVKNELKKDGVDVVLFEGINAGENDYSAVITKLK
                     SLNVDFVYYGGYHPEMGLLLRQGSEQGLKARFMGPEGVGNSQIAGIAGPAVEGMLLTL
                     PADFTKNPENAAIVKAFKDKGRDPGGAFQLTSYAAMESIVAGIKGAGVDDPVKVAAYL
                     HSHPVNTPIGKVAWNKQGDLTQFKFDVFVWHKDGSKTVY"
     gene            complement(4403..5359)
                     /locus_tag="VFR20_00725"
     CDS             complement(4403..5359)
                     /locus_tag="VFR20_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0229068.1"
                     /translation="MTLTELKYIVAVARERHFGRAAEACFVSQPTLSVAIRKLEDELG
                     VVLFERGGSEVGITPIGLRVVTQAQRVLEEGANLREIARQGHDPLAGPLRVGVIYTIG
                     PYLLPRLVPTQIAATPQMPLLLQENYTTRLLELLRQGEIDCAIVALPLPEYGLMVRPL
                     YDEPFLVAVPRHHAWAKRKEIHSSELKDETMLLLGTGHCFRDQVLEVCPELSRYSASS
                     EGIQRTFEGSSLETIRHMVAAGIGITVLPASSLSMPGMQSDLLAYIPFKTPVPDRRVV
                     LVWRKSFPRPTAIEALITSVHACGLPGVNYVSDAQAHELVEA"
     gene            complement(5393..7465)
                     /gene="recG"
                     /locus_tag="VFR20_00730"
     CDS             complement(5393..7465)
                     /gene="recG"
                     /locus_tag="VFR20_00730"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU0229069.1"
                     /translation="MSSVARPRGRAAQASGASPTEARLRQLGLRSAEDFVVHLPLRYE
                     DETRVTPVSSLRPGMTAQVEGHVVDSDVQYRPRRQLHATLADETGTLNLRWLNFYGSQ
                     QKQLEPGRLLRVRGEVRAGFSGLEMVHPKVGRAEGALPESLTPVYPTTQGLTQLTLRR
                     LIDDALKRADLSDTLPEAIRLQYGLVPFNEAIEFLHHPPPDISYSELVEHEHPAWRRI
                     KFDELLAQQLALAAARARRRAQHARPLLADKGLVDRFRGSLPFSLTPAQIRVVAEIGA
                     DLGRDFPMLRLLQGDVGSGKTVVAALAAAQAMDAGAQVAIMAPTEILAEQHFRKLVGW
                     LEPLGVRVAWLTGSQKTGERRASAGRIANGDAQLVVGTQALIQDKVEFADLGLVILDE
                     QHRFGVDQRLSLSRKGEAGSPLDEWVPHQLHMSATPIPRSLAMTFLADLDVSVIDQLP
                     PGRQPVLTKLVVDTRRDEVLDHVADEVRQGRQAYWVCPLVEESEALQLQTAVDTHLAL
                     TAALPDLRVGLVHGRLPASDKNQVMHAFQAGDIDVLVATTVIEVGVDVPNASLMVIEH
                     AERFGLAQLHQLRGRVGRGSARSACVLLYQAPLSLIARQRLRAMYETTDGFEIARRDL
                     EQRGPGEFLGVRQSGATLFRFANFETDGQLVELAREAAAELNNHFPDWAGVHLRRWAR
                     GREDFLRS"
     gene            complement(7485..7874)
                     /locus_tag="VFR20_00735"
     CDS             complement(7485..7874)
                     /locus_tag="VFR20_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018574853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEU0229070.1"
                     /translation="MAKQIIHTDKAPAAVGPYSQAVATNAGRTVYLSGQIGLEPGTGE
                     LISENFEGQVRQSFANMQAVIEEAGGTLADIVKLTLFLTDLSKFASANAIMADVIPQP
                     FPARSTLGVASLPKGAQFEVEAVVVLS"
     gene            complement(7992..8768)
                     /locus_tag="VFR20_00740"
     CDS             complement(7992..8768)
                     /locus_tag="VFR20_00740"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEU0229071.1"
                     /translation="MAAFNSEQVLSVHHWNDTLFSFTTTRDAALRFHNGHFVMLGLEV
                     DGKPLTRAYSIASANYEDNLEFLSIKVQNGPLTSRLQHLKAGDSVLVSRKPVGSLLVD
                     DLKPGKNLYLFATGTGLAPFLSIIKDPDVYERFEKVVLIHGVRFANELAYADFIQGEL
                     PRNEYFGESVRNKLVYYPTVTREPFRNQGRITHLVETGRLFKDIGLPPLNPETDRAML
                     CGSPHMLADIGGMLDARGFVVSSGVGHAGDYVIERAFVEK"
     gene            complement(8980..10605)
                     /locus_tag="VFR20_00745"
     CDS             complement(8980..10605)
                     /locus_tag="VFR20_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEU0229072.1"
                     /translation="MKPVWVADDDQSIRWVLEKALARVSIPCRCFASGDEVLEALESE
                     TPSVLVTDIRMPGGDGLALLGRVKALHPTLAVIVMTAYTDLDSTVAAFQRGAFEYLVK
                     PFDLNMAVSLIQRALRENESLGDSIPAEVSGADGVADRLLESGGKAEALDANGRRQAG
                     SEPTGFGAVRSLAVEAPESGGSRGGGGARVMMQSSSPAMQDVFRAIGRLAASPASVLI
                     TGESGTGKELIARALHEHGVRAKGPFIAVNAAAIPRDLLEAELFGHERGAFTGATQLR
                     RGRFEAAHGGTLFLDEIGDMSLELQTRLLRVLAQGSFYRVGGSQAIDVDVRIVAATHQ
                     PLERLVAEHAFREDLFHRLNVIRLRLPPLRERRDDIPALAGLFLKNSAEELRVPVRRL
                     SPAARQVLCDFDYPGNIRQLENFCHWVTVMSAGQLVDAKDLPPELSHPVPAESRVAAA
                     VSVGSQPPPPAEMTWLELLAAEVTARLSRGEPALMSSLTRDFERVILEAALRHSQGRR
                     AEAAQRLGIGRNTLTRKHKELGMDDGSEETGSA"
     gene            complement(10602..11648)
                     /gene="glnL"
                     /locus_tag="VFR20_00750"
     CDS             complement(10602..11648)
                     /gene="glnL"
                     /locus_tag="VFR20_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEU0229073.1"
                     /translation="MDPAALDLLSTGVVLLDEQAAIRHVNVSTEELIGRSRRQLLGSA
                     GDAWFEADAGWKSHFARAVRGELGVVSQDVPVLRSGRMLNLSIVPLHAQPWAAVLELR
                     PVDQHAALERQQLLARELDVQRESLRNLAHEIKNPLGGLRGAAQLVEAEFDVPGLRDY
                     TRVIIAEADRLSTLVDRLLVSQGQPRLTEKFNVHEICERVYTLVAAEFGADVRLLRDY
                     DASVPDLRGDASRLLQSLLNIVRNAAQALVENPSAIQRCVIVRTRVGRQPAMPAHGGA
                     LGLIISVVDNGPGVPVELRDRIFHPLVTGRAQGTGLGLSLAQEYVQQMGGLLEFDSDP
                     GHTEFRLILSLERA"
     gene            complement(11737..13149)
                     /gene="glnA"
                     /locus_tag="VFR20_00755"
     CDS             complement(11737..13149)
                     /gene="glnA"
                     /locus_tag="VFR20_00755"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU0229074.1"
                     /translation="MSTPDDIIKMIAEREVAFVDFRFSDTLGREHHVSVPAHAVDEDK
                     LETGQAFDGSSIPGWKGIEASDMALIPDLNTARMDPFREENTLILTCDVVEPSDLKGY
                     DRDPRSIARRAEAYLKSSGIGDAAYFGPEPEFFVFDGVTWDDNMSGCFVKVRSDEAPW
                     SRGIDSDSGNHGHRPGVKGGYFPVPPVDSFQDMRSEMCLLLEQQGVPVEVHHHEVAAP
                     GQLEIGTKFSTLVQRADWNQILKYTVHNVAHVYGKTATFMPKPIVGDNGSGMHVHQSV
                     WKDGQNLFAGNGYAGLSEFALYYIGGIIKHARALNALTNPGTNSYKRLVPHFEAPVKL
                     AYSARNRSASIRIPYVGSPKARRVEARFPDPLANPYLAFSALLMAGLDGVQNKIHPGD
                     PADKNLYDLPPEEDAKIPTVCSSLEQAVESLNQDREFLTRGGVFSNDMLDAYIDLKMA
                     EITRLRMTTHPVEFDLYYSL"
     gene            complement(13360..14583)
                     /gene="hemW"
                     /locus_tag="VFR20_00760"
     CDS             complement(13360..14583)
                     /gene="hemW"
                     /locus_tag="VFR20_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEU0229075.1"
                     /translation="MPRAVIPAGVEPVRIRPGGREPGSIRLTSLPPLSLYVHVPWCVR
                     KCPYCDFNSHEAGGELPEEDYLAALQADLEQCVPLVWGRQVVSIFIGGGTPSLLSERA
                     VDRMLAMFRACLNVLPDAEVTLEANPGTAEAGRFEGYARSGVRRISLGIQSFDDKALA
                     ALGRIHDGAQARRAIDMAQRAVERVNLDLMFALPDQSLDGCALDLRTAMSYGTGHLSF
                     YHLTLEPNTVFAKYPPAVPDDDLAAEMQELIAGETAASGWERYEVSAYARSGQRCLHN
                     VNYWEFGDYLGIGPGAHAKLSFHDRIVRRATLKNPVSWMAKAPLRDDSHLAENRTVGA
                     RDLPFEFMLNALRLKQGVMASTFAQRTGLSLLAVAPALERARAKGLLESDPQWLTATP
                     MGWRFLNDLLAEFLP"
     gene            complement(14587..15201)
                     /gene="rdgB"
                     /locus_tag="VFR20_00765"
     CDS             complement(14587..15201)
                     /gene="rdgB"
                     /locus_tag="VFR20_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEU0229076.1"
                     /translation="MSSHPNVVLASNNPGKLREFSAILDQAGLVMVPQGQLGIPEAAE
                     PFATFIENALAKARHASEHAGMPALADDSGLCARALGGAPGVYSARYASMAGGEKSDS
                     ANNRRLIGALAGQADLSAVYVAVLVYVRAADDPLPIIAQGLWHGEITLEPRGDHGFGY
                     DPHFYLPDLGRTAAELDPDEKNRVSHRARALRVLLESLREQGGA"
     gene            15378..16568
                     /locus_tag="VFR20_00770"
     CDS             15378..16568
                     /locus_tag="VFR20_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0229077.1"
                     /translation="MTHLSPKRRSMQATGLPVLALVIAGFWLLTHRRDGVADTPAKAT
                     PAVVATTVRARQQNIPDYLSGIGTVTARQTVTVRTRIDGQLEKIDFREGQDVKAGQLL
                     AQLDPRTQQAQLEQAKAQLAKDQALLANAELDLRRYQKLITLNATSRQILDAQRSQVA
                     QLRADIQADQAHISDAATQLSYTRITAPISGRVGARLVDQGNIVHTTDANGLVVINQI
                     DPITVVFTVPGDSFLAINHAMAAGEPLAVIVYPRDGTRSLGDGKLVLMNNQIDTSTGT
                     IMLKGLLPNPGHDLWPGQYVDVRLVLGERRGAITVPAAAVQRGPDGLYVYTVDKHDIA
                     HMQPITVAHRQQGLAIIDKGLQADQRVVIDGQYRLKPGTRVTEAPHRATESTGSENPR
                     SGSN"
     gene            16565..>19238
                     /locus_tag="VFR20_00775"
     CDS             16565..>19238
                     /locus_tag="VFR20_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0229078.1"
                     /translation="MSISANFIKRPIGTSLLALALLLAGMVAWRLLPVAPLPQVDFPT
                     ILVSASLPGGNPETMASTVAQPLERQFSEIPGLDQMTSISALGTTQITLQFNLNRNID
                     AAALDIQAAINAAAGQLPHDLPSPPSFRKINPADWSILILSVQSRAMPLIEVDNYADN
                     LLAQQISRIQGVGQVNIFGEQKPAIRIQVDPRKLASMGLTLEDIRGVIAETTVSQPKG
                     TLDGDHQSFTVYTNDQLTEAAKWNDTILAYRHGAPIRVRDIGRAIPGPEDVKQAAWAF
                     AGKAAPKTDAPINGRSLIIGIHKEPGANVLETVRRIKAALPRIEASLPASVHVSTLID
                     RTQNIKASVFDVEMTLLLSIALVTLVIFMFLRSVPATLIPAVTVPMAILGAAVVMYLL
                     GYSLDNLSLMALTISVGFVVDDAIVMLENIYRHIEEGMSPLEAAYKGAGEIGFTIVSI
                     SVSLVAAFIPLLLMGGIVGRLFREFAVTVALTILVSVIVSLTLTPMLCSRFIRHYDRA
                     SHGRVYLVFERGFDHMLAGYRRGLHWVLRHPFLTLCSFLATLLATVALFITIPKGFFP
                     QQDTGYIHGFAEGPQDTSFGAMSRRMEALADVVRQDPDVATFGMSAGRTTFNTGTFFI
                     GLRPKDQGRTVSANQIIDRLRPKLEKVEGARLFLQAGQDINVGGRLSRTQYQYTLTDA
                     NLDELNTWAPRLLARFRQLPELTDVATDQQNQAATAVVTIDRARASTFGITPAMIDST
                     IYDAIGQRQVAQFFTQLNSYEVVLEATPQLQKDSDLFRTLYVRSPISGDLVPLSSFVT
                     VNTSKTGYLSINHQGQFPAVTLSFNLAPGVALGQAVDAIRHARADMNIPQTLSGSFQG
                     TAKAFGESLATQPYLIAAALISVYIVLGLLYESYI"
BASE COUNT         3766 a         6245 c         5712 g         3515 t
ORIGIN      
        1 gccccgtacg cggggcgcac cactttgggg gatatgcaag aatgcagggt tttcgcatac
       61 tcttggccgc cgatgggcgc ggtatgacgc ggaaccgggt cagcccctgg accgatgcgg
      121 ggcacggacg cggcgcctgt caggcgcggg cgagcgcaca actaaacaaa gcggaatatg
      181 gaaaaacgcg tcgtcttctc tcataaaaca ctgccctacc ttttgctgat cccgcaactg
      241 gccgtcaccg ttgtcttctt cttcctgccg gccggccagg ccatgtggca gtcgttccgt
      301 ctcgaagacg cgttcggcct gtcctcccaa ttcgtaggtt ggcagaactt catcgacctg
      361 ttctcgaatc cggactacat caattccttc aaggtgacca ccgagttttc gttgctggtc
      421 gccatttccg gcctggccat tgccctgctg ctggccgtca tggccaaccg cgttgtcaag
      481 ggcgccgggc tgtacaagac cctgttgatc tggccttacg ccgtggcgcc cgccgtggcc
      541 ggcgtgctgt ggctgttcct gttttcaccg gctgtcggta tcctggccgt gtatctggcc
      601 gaaatgggct ttcactggaa tccgcggctc aacggccacg atgccatgac gctggtggtg
      661 ctcacgtcgg tgtggaagca gatttcctat aacttcctgt tcttcctggc cgggcttcag
      721 tccattccgc gatcactgat cgaagcggcc gccatcgacg gagcgtcgcc gttccggcgc
      781 ttctggacca tcgtgttccc cctgttgtcg cccacgacct tcttcctgct gatcatcaac
      841 gtcatctatt cgttcttcga caccttcgcc atcatcgacg tcatgaccca gggtggcccg
      901 ggcaactcca cgtccatcct ggtctaccag gtgtacaacg cggggttcaa gggtatggac
      961 atcgggtcat ccgccgccca gtcggtgatc ctgatgatca tcgtcgtggc gctgaccgtg
     1021 atccagttcc gctacatcga ccgaaaggtc aattactgac acggccctca aggcaccacc
     1081 atgatcgaac gccgccccta tctggacatc ttctcccacg tcatcctgct tcttggcgtg
     1141 gccatcgtgg cctttccact gtatgtcacg ttcgtggcct ccacgcagac ggcacaggaa
     1201 gtggcccgcg ccccgatgtc gctgctgccc ggcagccact tcctgcagaa ctacgcatcc
     1261 gtgctgttcg ggcagggcag cggctcggtg cccgtggccc gcatgatgtg ggtcagcacc
     1321 gtcatggccc tggtgatcgc catcggcaag atcgtcattt ccatgctgtc ggccttcgcc
     1381 gtggtgtact tccgcttccg cttccggatg ttcttcttct ggatgatttt cgtcacgctc
     1441 atgctgccgg tggaagtccg catcgctccc acctacaagg tggtggccga cctgggcatg
     1501 ctgaacacct acgcgggcct gacactgccg cttatcgcgt cggcaacagc cactttcctg
     1561 ttccggcagt ttttcctgac catccccgac gagctcatcg aagcggcgcg catcgacggt
     1621 gccggcccca tgagattttt ccgcgacatt cttttcccac tttccaagac cagcatcgcg
     1681 gcgttgttcg tgatccagtt catttacggc tggaaccagt atctgtggcc tttgatcatc
     1741 accacgcacg aggacatgta ccccatcgtg atcggcatcc agcgcatgat cgcggggggt
     1801 gacagtgtga catcatggaa tctggtcatg gccacggcgc tgctggccat gctgccgccc
     1861 gcggcggtcg tcatcctgat gcagaaatgg ttcgtcaagg gcctggtcga cgccgaaaaa
     1921 tagccagccc cacattcttc cgaacatcac tgaaacaccg gaatccagac aacatggcca
     1981 cactgaattt tcgcaacgtc accaaaacct atgccggcgg cgttgccgtg atccacggca
     2041 tcagcatgga tgtcaccgat ggcgaattca tcgtcatcgt gggaccgtcc ggttgtggaa
     2101 aatccacgct gatgcgcatg gtcgcgggcc tcgagacggt caccggcggc gacatcgtca
     2161 ttggcgacaa ggtcgtcaac acgctggagc cggccgagcg cgacatcgca atggtgttcc
     2221 agaactatgc cctgtacccg cacatgtcgg tgttccagaa catggcctac ggattgaaga
     2281 tccgcaagct cgacaaggaa gacatcaagc gccgcgtgga cagcgcggcc gagatcctcg
     2341 aaatcggcca tctgctcgag cgccgacccg gccaattgtc cggcgggcag cgccagcgtg
     2401 tggccatggg gcgcgccatc gtgcgcgaac ccaaggtatt cctgttcgac gaaccgctgt
     2461 caaacctcga cgccaaattg cgcgtgcaga tgcgcctgga aatccagaag ctgcatcgcc
     2521 ggctcaagac caccagcctc tacgtcacgc acgaccaggt cgaggcgatg acgctcgccc
     2581 agcgcatggt ggtcatgaac aaaggggtgg ctgaacagat cgggacaccc atcgaggtgt
     2641 ttgaaaaccc cgcgtctacg ttcgtcgcca gcttcatcgg gtcgccaccc atgaatctga
     2701 ttcccgtcga gatcagcccg gaaagaaccc tccgcggcgt ggacggtacg ccgttcgatc
     2761 tcgaacccgc ccaggtgccg gcggatctgg ccggccgcaa ggccatcctg ggcattcggc
     2821 ccgagcacat gcggctgcgc aagcccggca tcccggtgga catcgaaatg gtcgaagtgc
     2881 tgggatccga gcaactgata cacggccgca tcggcgatac acaactggtg gtgcgctgtc
     2941 ccgtcagcct gacgcgcgat caaccggtgc gcgtgggtga tgcattcccg gttggttcag
     3001 acggggtgca tccgctgcac tggttcgacg ccgggacggg caaacgtata gtcgaatgag
     3061 catctgacct acaaagcagc gtcccctcaa taatcggtaa aaaagccgcc aaccctgaaa
     3121 cggactggcg gcttctttac gtcggcccgc gcaaaccgcc ctatccgggg ctggtgcgcg
     3181 agcccgccga ttattaataa acggtcttgg aaccgtcctt gtgccagaca aaaacgtcga
     3241 acttgaactg ggtcagatcg ccctgtttgt tccaggcgac tttgccaatg ggggtgttga
     3301 cgggatggga gtgcaggtag gcggcgacct tgacggggtc gtcgacaccg gcacccttga
     3361 tgccggccac gatggattcc atggcagcat acgaggtgag ctggaacgcc ccaccgggat
     3421 cacgcccctt gtccttgaac gctttgacga tggccgcgtt ctctgggttc ttggtgaagt
     3481 cggccggcag cgtgagcagc atgccttcca ccgcgggacc ggcgatgcct gcgatttgtg
     3541 aattgcccac accctcgggc cccatgaagc gggctttcaa gccttgctcc gagccctggc
     3601 gcagcagcag gcccatttcg ggatggtagc cgccgtagta gacgaaatcg acattgagcg
     3661 acttgagctt ggtgatgacg gcggaataat cgttttcgcc ggcattgatg ccttcgaaca
     3721 gaacgacgtc gacgccatct ttcttcagct cgttcttcac agccgtggcg ataccctggc
     3781 catacgactg cttgtcatgc aggacggcca cgcgcttggg cttgatcttg tcggcgatga
     3841 acttgccggc caccgggcct tgctgatcgt cgcgaccgat ggtgcggaag atcatgtcgt
     3901 agtgcttgcc atccgtgacc agcggcgacg tggccgacgg cgagatcatc agcaccccgt
     3961 tgttgttgta gatgtcggtg gcggcgatgg tggcgcccga gcacacatgg cccacaacgt
     4021 aatggatgtc gtcattgacg acgcggttgg ccaccaccgg cgcctgcttg ggctcgcagc
     4081 catcgtccat gacgactacc tcgagcttct tcccgagaac gccgccggcg gcgttcactt
     4141 tttcgacttc agtgtcgacg ccctgcttga ccatgccgcc gtattgcgtg agaggccccg
     4201 tgaacggacc cgcgacagcg atcttgatgg tgccggccgc attggctccg gaagaaacca
     4261 gcatgccaac agtgatgccc aacgcagcaa cgataggcgt gaaacgaatt ttcataaagc
     4321 ctcccttttt agttccgggc aaggatacac taaaaaggca ccgcgaaaaa ccggcgccga
     4381 caccggtttt gggcccaggc gatcaggcct cgaccaattc gtgcgcttgc gcgtcgctga
     4441 cgtaattcac cccgggcaag ccacaggcgt gcacggaagt gatcagcgcc tcgatagccg
     4501 tgggacgagg gaaactcttg cgccacacca gcaccacacg ccggtcgggc accggcgtct
     4561 tgaacgggat gtaggccagc agatcgctct gcatgcccgg catcgacagt gaactggcgg
     4621 gcagcaccgt gatgccaatg cccgccgcga ccatatgccg gatggtctcg agcgaggaac
     4681 cctcgaaggt gcgttgtatc ccttcactag aggccgagta ccgcgaaagc tcggggcaga
     4741 cctcgagcac ctgatcgcgg aaacaatgcc cggttcccag cagcaacatg gtttcatcct
     4801 tgagctcgga tgaatggatt tccttgcgtt tcgcccaggc gtgatggcgc ggcacggcca
     4861 caagaaacgg ctcgtcatat agcggccgca ccatcaggcc gtactccggc aggggaagcg
     4921 ccacgatggc gcagtcgatc tcgccctgac gcagcaattc gagcaatcgt gtggtgtagt
     4981 tctcttgcag cagcagcggc atctgaggtg tggctgcgat ctgagtgggc acgagacggg
     5041 gcagcaggta aggcccgatg gtgtagatca cacccacgcg caacggccca gccaacgggt
     5101 catggccttg gcgggcgatc tcgcgcagat tggcgccctc ttccagaaca cgttgcgcct
     5161 gggtgacgac ccgcaagccg attggtgtga tgcccacctc ggaaccaccg cgctcgaaca
     5221 gcaccacacc cagttcatcc tcgagtttgc ggatcgcgac ggacaaggtg ggctgactca
     5281 cgaaacaagc ctcggcggcg cgcccgaaat gacgctcccg ggccaccgcc acaatgtatt
     5341 tgagttccgt cagagtcata tcgctttcca gagtgaaata aggctgtgca catcaactgc
     5401 gcaggaaatc ctcccggcca cgggcccagc gccgcaaatg cacgccggcc cagtccggga
     5461 aatgattgtt caattccgcc gccgcctcgc gcgccagctc gaccagttgt ccgtcggttt
     5521 cgaaattcgc aaagcgaaac agtgtggcgc cagactgccg cacccccagg aactctccgg
     5581 ggccgcgctg ctccagatcg cggcgggcga tctcgaaacc atccgtcgtt tcgtacatgg
     5641 cgcgcagacg ctgccgcgcg atcagggaca gtggcgcctg gtacagcagc acgcaggccg
     5701 atcgggccga tccccggcca acccggccgc gcagttggtg caactgcgcc agaccgaacc
     5761 gttcagcgtg ctcgatcacc atcagcgagg cgttgggcac gtccacgccg acttcgatca
     5821 cggtcgtggc caccagaacg tcgatgtcac cagcctggaa cgcgtgcatg acctggttct
     5881 tgtccgatgc cggcaaccgc ccgtgaacca atcccacacg taaatctggc aaggcggccg
     5941 tcaacgccag atgagtgtcc acggcggtct gcaactgcag ggcctcgctt tcctcgacca
     6001 gcgggcagac ccagtaggct tgcctgccct ggcgcacctc atcggcgacg tgatcgagca
     6061 cctcgtcgcg gcgtgtatcg accaccagct tggtcagcac gggttggcgc cctggcggca
     6121 actggtctat gaccgagacg tcgaggtcgg cgaggaacgt catggccaga ctacggggaa
     6181 tcggcgtggc gctcatgtgc aactggtgcg gaacccactc gtccaatggg gaaccggctt
     6241 cacccttgcg gctcaggctc aatcgctgat cgacaccgaa ccggtgctgt tcgtcgagaa
     6301 tgaccaggcc cagatccgcg aactcgacct tgtcttgaat gagcgcctga gtgcccacca
     6361 ccagttgtgc gtcaccattg gcgatgcgtc cagcgctggc gcggcgctcc ccggtcttct
     6421 gacttccggt gagccaggcg acccgaacgc ccagcggctc cagccacccg acgagtttgc
     6481 gaaaatgctg ttccgccaga atttcagtcg gggccatgat cgccacctgc gcgccagcat
     6541 ccatggcttg cgccgcggcc aaggcggcca cgacagtctt gccgctgccc acatccccct
     6601 gcaacaggcg cagcatgggg aaatcgcggc cgagatcggc accgatctcg gccaccacgc
     6661 gaatctgtgc cggcgtcaat gaaaatggca ggctgccccg aaaacgatcg accagccctt
     6721 tgtcagccaa cagaggccgg gcatgctgcg cgcgccgccg tgccctggcc gccgccagcg
     6781 ccagctgctg cgccaacaat tcgtcgaact tgatacgacg ccaggctgga tgttcatgct
     6841 cgaccaattc ggaatacgaa atgtccggcg gcggatgatg aaggaattcg atggcctcgt
     6901 tgaacggcac caacccatat tgcaaccgaa tggcctcggg cagggtgtcg gacaaatcgg
     6961 cgcgcttgag cgcatcatcg atgagacgcc tgagcgtcag ctgcgtcaga ccctgcgtgg
     7021 tgggatacac gggggtgagg gattcgggca gcgctccttc ggcacggcca accttggggt
     7081 gcaccatttc cagccccgaa aaacccgccc gcacctcgcc gcgcacgcgc agcaagcggc
     7141 cgggttccag ttgcttctgc tggctgccgt agaaattgag ccagcgcaga ttcaaggtgc
     7201 cggtttcgtc ggccagggtg gcatgaagct ggcgtcgcgg ccggtactgc acgtcagagt
     7261 ccacgacatg cccctccacc tgcgcggtca tgcccggccg cagggacgac acgggcgtga
     7321 cccgggtttc gtcctcgtag cgcagcggaa gatgcacgac gaaatcctcg gcgctgcgca
     7381 gccccaattg ccggagccgg gcttctgtgg gcgacgcccc cgaggcctgc gctgcgcggc
     7441 cacggggccg tgccacggaa gacatgactg gagaaccgaa tcggttagga aagaacgacg
     7501 acggcttcca cttcgaactg cgcgcccttg ggcagactcg ccacaccaag ggtcgagcgc
     7561 gcggggaacg gctggggaat cacgtcggcc atgatggcgt tggcgctcgc gaacttgctg
     7621 agatcggtca agaataaagt gagtttgacg atgtcagcca gtgtcccgcc cgcctcttcg
     7681 atgacggcct gcatgttcgc gaacgactgg cgcacctggc cctcgaagtt ttcggaaatc
     7741 agttcgccgg tgcccggttc cagcccgatt tgcccggaca ggtagacggt tcgccccgca
     7801 ttggttgcga ccgcctggga atacggtccg accgccgccg gcgccttgtc ggtgtggatg
     7861 atttgttttg ccatggtgat ttgtctctga agatgtgaaa acgtcaacaa ctatcagagt
     7921 ttaatcatct atagctaaca ttaaaaggct ttcaggcgcg cagatcggat ttatttctcg
     7981 gagaaggcgt tttatttctc gacgaaggcg cgttcaatga catagtcgcc cgcatgcccc
     8041 accccggaag acaccacgaa accacgcgca tcgagcatgc cgccgatatc ggcaagcatg
     8101 tgagggctgc cgcagagcat ggcccggtcg gtctcgggat tcaggggcgg caggccgatg
     8161 tccttgaaca accgacccgt ttccaccaga tgcgtgatcc gtccttggtt ccggaacggc
     8221 tcccgtgtca cggtcgggta gtacacgagc ttgttccgca ccgattcgcc aaagtactca
     8281 ttccggggca attcgccttg gatgaaatcg gcgtaggcga gctcattggc gaaacgcacg
     8341 ccgtgtatca gcacgacctt ctcgaagcgt tcgtagacat ccgggtcctt gatgatgctt
     8401 aagaacggtg ccaggccggt gccggtggcg aacagataca ggttcttgcc gggtttgagg
     8461 tcatcgacca gcagtgatcc gaccggcttg cggctgacca acaccgagtc acccgccttc
     8521 aggtgctgca ggcgcgacgt caacggcccg ttctgcacct tgatgctgag gaattccagg
     8581 ttgtcttcat aattggcgct ggcgatgctg taggcgcgcg tgagcggctt gccatcgacc
     8641 tccaggccca gcatgacgaa gtggccgtta tggaacctga gtgcggcatc gcgcgtggtg
     8701 gtgaaggaaa acaacgtgtc gttccagtga tggacactga gaacctgttc ggaattgaat
     8761 gctgccatat cgcacaatga aagtcgtggc ggcccagacg gccggcgcga gaatgccgac
     8821 cgtcaaggat cctctgattc agaggattct tgacgccaac catgggaatg gctcatttta
     8881 acccaccgtg acaagacgac tccgcggcca ccaccagccg ttcgcggtgc ttgtcgaagt
     8941 cgcctggcat gtgccgggca tatgccggaa cgctcgatgc tacgccgacc ccgtctcttc
     9001 ggaaccgtca tccatcccca gttccttgtg cttgcgcgtc agcgtgttgc gtccgattcc
     9061 caatcgctgt gccgcctcgg cgcgccggcc ttggctatga cgcaaggcgg cttccagaat
     9121 gacgcgttca aaatcccgcg tcagcgatga catgagtgct ggctctccac gggacaggcg
     9181 ggccgtcacc tccgcagcca gaagttccag ccaggtcatc tcggctggcg gcggcggttg
     9241 cgatccaacg ctgaccgcgg ctgccacccg gctctccgcg ggaaccgggt gcgacagttc
     9301 cggtggcaaa tccttggcgt cgacgagctg gcccgccgac atgaccgtga cccaatgaca
     9361 gaaattctcc agttgacgga tgtttcccgg ataatcgaaa tcacacagaa cctgtcgggc
     9421 cgctgggctc aggcgccgca caggtacgcg cagttcctcg gcgctgttct tcagaaacag
     9481 acctgccagg gccggaatat cgtcccggcg ctcgcgcagc ggcggcaggc gcagccgaat
     9541 gacattcaac cgatgaaaca aatcctcgcg gaacgcatgc tccgcgacca atcgctccag
     9601 gggctgatgc gtggccgcca cgatgcgcac gtccacatcg atggcctggg atcctccgac
     9661 ccgatagaaa ctgccctgtg ccaacacgcg tagcaggcgc gtctgtagtt ccagcgacat
     9721 atcgccgatt tcatccagga acagtgtccc tccgtgggcc gcctcgaacc gaccccgccg
     9781 caactgtgtc gcgccggtga aagccccccg ctcgtggccg aacagctcgg cctcgagcag
     9841 atcgcgcggg atcgctgcgg cgttgacggc gatgaacggc cctttggcgc gcaccccgtg
     9901 ttcatgcagc gcgcgggcga tcagttcctt gccggttccc gattcaccgg tgatcaacac
     9961 actggctggc gacgcggcca agcggccgat ggcgcgaaag acatcttgca tcgccggcga
    10021 cgacgactgc atcatcactc tcgcgcctcc cccgccccgc gaaccgccgc tctccggcgc
    10081 ctcgacagcc aaagagcgga cggcgccgaa cccggtgggt tcgctcccgg cttggcgacg
    10141 cccgttggca tccagggctt cagctttgcc accgctctcc agcagccgat ccgccacgcc
    10201 atccgccccg gacacctcgg ccggaatgga atctcccaag gattcgtttt cccgcagcgc
    10261 gcgctggatc agggacaccg ccatgttgag atcgaacggt ttgaccagat actcgaacgc
    10321 gccgcgctgg aacgcggcga ccgtgctgtc cagatcggtg taggcggtca tgacgatgac
    10381 agccagggtc ggatgaagcg ccttgacacg ccccagcaga gcgagcccgt cgcctcccgg
    10441 catccggata tcggtgacca ggaccgaggg cgtctcggat tcgagtgcct cgagcacctc
    10501 gtcccccgag gcaaaacaac ggcacgggat cgacacccgc gccagcgctt tctccagcac
    10561 ccagcgaatg ctttgatcat cgtcggcgac ccagacgggt ttcatgcgcg ctccagagac
    10621 aagataagac ggaattcggt gtgtccggga tcggaatcga attccagcag accgcccatc
    10681 tgttgcacat attcctgggc caggctcagc ccaagacccg tgccctgggc tcggccggtg
    10741 accaagggat gaaatatccg gtcacgcagt tcgaccggca cacccgggcc gttgtccaca
    10801 acggaaatga tcagaccgag ggccccccca tgggcgggca tggccggctg acggcccacg
    10861 cgtgtgcgca caatgacgca gcgctggata gccgaaggat tctcgaccag agcctgcgcg
    10921 gcgttgcgca cgatattcag caacgactgc aatagccgcg aggcatctcc acgaaggtcg
    10981 ggcaccgacg catcgtaatc gcgcagcaac cgcacatcgg cgccgaattc cgcggcgacc
    11041 agcgtataga cgcgctcaca gatttcatga acgttgaatt tttcagtcaa ccggggctgg
    11101 ccttgcgaga caagcagacg atcgaccaac gtactgaggc gatccgcctc ggcgatgatg
    11161 acgcgcgtgt aatcgcgcag gcccggcacg tcgaattcgg cctccacgag ttgcgcggcg
    11221 ccacgcaggc cgccaagtgg attcttgatt tcgtgggcga gattgcgcaa cgattcgcgc
    11281 tgcacatcca gctcccgggc caaaagctgc tggcgctcca gcgcggcgtg ctgatcgacc
    11341 ggacgcaatt ccagcacagc cgcccagggt tgggcgtgca gtggcacaat ggaaagattg
    11401 agcatacgcc cggaacgcaa gaccggcacg tcctggctca cgacacccag ttcgccccgc
    11461 accgcgcgcg cgaaatgtga tttccagccc gcatcggcct cgaaccacgc atcgcccgcc
    11521 gaaccgagca actgccggcg cgaccgtccg atcagttcct cggtggaaac attgacgtga
    11581 cggatcgccg cctgctcatc gagcaggacg acaccggtcg agagcaggtc gagagcagct
    11641 ggatccatgt ctgaacctga acgggcgcgg gaaccctccc cgcgccccgg caccgccggc
    11701 atcacgagcc ccctgggggc ccgcgccggg ctggccttag agggaataat acagatcgaa
    11761 ctcgaccgga tgggtcgtca tgcgcaggcg ggtgatttcc gccattttca gatcgatgta
    11821 cgcatcgagc atgtcattgc tgaacacgcc gccacgggtg aggaactcac gatcctgatt
    11881 cagcgactca accgcctgct ccagagagga gcacacagtc gggatcttcg catcttcttc
    11941 cggcggcaga tcgtacaggt tcttgtcggc gggatcgccg gggtggatct tgttctgcac
    12001 gccatccagg ccggccatca gcaatgccga gaacgccagg tacggattgg ccagcggatc
    12061 ggggaagcgg gcctcgacac gacgcgcctt ggggctgccc acatacggaa tgcggatcga
    12121 cgccgagcga ttgcgcgccg aatacgccag cttgaccggg gcctcgaagt gcggcaccag
    12181 gcgtttgtac gaattggtgc ccgggttggt cagcgcgttg agcgcgcgcg catgcttgat
    12241 gatgccaccg atgtagtaca acgcgaactc ggacaggccc gcgtaaccgt tgccggcgaa
    12301 cagattctgg ccatccttcc agaccgactg atgcacgtgc atgcccgaac cgttgtcgcc
    12361 gacgatgggc ttgggcatga atgtggccgt cttgccgtac acatgggcga cattgtgcac
    12421 cgtgtacttc aggatctggt tccagtcggc gcgctgcacc agggtgctga acttcgtgcc
    12481 gatttcgagc tggcccggtg cggcgacctc gtggtgatgg acttcgaccg gaacgccctg
    12541 ttgttcgagc aacaggcaca tttccgaacg catgtcctgg aacgagtcga cgggcggaac
    12601 ggggaaatag ccgcctttga cgcccgggcg atggccgtga ttgccggagt cggagtcgat
    12661 gccacgcgac cagggcgctt cgtcggagcg cactttcacg aagcaacctg acatgttgtc
    12721 atcccaggtc acgccgtcga aaacgaagaa ttcgggttcc ggaccgaagt acgcggcgtc
    12781 gccaatgccg ctggacttca ggtaggcctc cgcgcggcgc gcgatcgagc gcggatcgcg
    12841 atcatagccc ttgagatcag acggttcgac cacatcgcag gtcaggatca acgtgttttc
    12901 ttcacggaac gggtccatgc gggccgtgtt cagatcgggg atcagcgcca tgtcggacgc
    12961 ttcgataccc ttccagccgg ggatggacga tccgtcgaac gcctggcccg tctcgagttt
    13021 gtcttcgtcc acagcgtggg ccggaacgct gacatgatgc tcacgcccca gcgtatcgct
    13081 gaagcggaag tcgacgaacg cgacttcgcg ctctgcaatc atcttgatga tatcgtcggg
    13141 agtcgacata aatgctcctg aaaaaggaaa taggtaccgg gagatagact cgatttatac
    13201 aagcaataat cgtgccaatt tccgaaaatc ccattacatc gcggccccgg gccagcgtcg
    13261 atgcaaacgg gtgcggcaag gtatgtctca ccagataatg caccaaaata gtgcaattaa
    13321 tgaggcataa tagcaccatt ttggtgcgaa ccaacctcgt caaggcagaa attccgccag
    13381 caagtcattc agaaaacgcc atcccatcgg agtggccgtc agccattgcg gatcggattc
    13441 caatagcccc ttggcacgtg cgcgttcgag cgcgggggct accgccaaca gggacaaccc
    13501 agtgcgttga gcgaatgtcg aggccatgac accttgtttc agccgcagcg cattgagcat
    13561 gaactcgaac ggcagatccc gcgcgcccac ggtgcggttc tcggccaagt ggctatcgtc
    13621 ccgcagaggg gccttggcca tccaggacac ggggttcttc aacgtggcgc gccgaacgat
    13681 gcgatcatga aacgacagct tggcatgcgc ccccgggcct atgcccaggt aatcaccaaa
    13741 ctcccaataa ttcacattat gcaggcagcg ctgcccggat cgcgcgtagg cggacacctc
    13801 atagcgctcc cagcccgacg cggcggtctc ccccgcgatc agctcttgca tttccgccgc
    13861 cagatcatca tcaggcacgg cgggaggata cttggcgaaa accgtattcg gctcgagtgt
    13921 cagatggtag aacgacaggt gacccgtgcc atacgacatg gcggtgcgca gatcgagggc
    13981 gcagccgtcc agcgactgat cgggcagcgc gaacatcagg tctagattga cgcgctcgac
    14041 ggcgcgctgc gccatgtcta tcgcccgtcg agcctgcgcg ccatcatgga tgcgacccaa
    14101 tgcggccagc gccttgtcgt cgaagctctg gatgcccagc gaaatacgtc tgaccccgct
    14161 acgggcatag ccttcgaacc gccccgcttc tgccgtgccg ggattggcct cgagcgtgac
    14221 ctcggcatcc ggtaacacat tcaaacaggc gcggaacatc gccagcatgc gatccaccgc
    14281 ccgttcggaa agcaagctgg gcgttccgcc gccgatgaag atggagacga cctgccgccc
    14341 ccacaccagt ggcacgcatt gctccaggtc agcctgcaga gcggccaggt agtcttcttc
    14401 cggaagctcc ccgcctgcct catgggaatt gaagtcacag tatggacact tgcgcacaca
    14461 ccagggcaca tgcacgtaca gagacaacgg cggcaggcta gtcagccgga tgctgcccgg
    14521 ctcccgtccg cccggacgaa tgcgcaccgg ctccacgccc gcgggaatca cagctcgcgg
    14581 catgtctcag gccccacctt gttcacgcaa ggattccagc aatacgcgca gtgcccgggc
    14641 gcggtggctc acgcggtttt tttcatcagg gtcgagctcg gcggcggtgc gccccagatc
    14701 gggcagataa aaatggggat catagccaaa accatgatct ccccggggtt ccagggtgat
    14761 ttcaccgtgc cagagccctt gcgcaatgat gggcagcggg tcgtcggccg ctcgcacgta
    14821 caccaggacg gccacataga cagccgagag atcggcctgg cctgccaggg caccgatcag
    14881 gcgtcgattg ttggcggagt cggatttttc gccgccggcc atcgaggcgt agcgtgccga
    14941 atacacaccc ggcgccccgc ccagggcgcg cgcgcacaac cccgaatcat cggccagcgc
    15001 cggcatgccg gcatgttcac tggcatgacg cgccttggcc agggcattct cgatgaaggt
    15061 cgcgaacggt tcggcggcct cgggaatgcc aagctggcct tggggcacca tgacaaggcc
    15121 ggcctggtcc agaatggccg agaactcccg cagtttgccg ggattgttgg acgcgagaac
    15181 gacgttggga tgggaagaca tgaaaattga acagggttca ccgattgaag gacttcgctt
    15241 cacgggccag atgacgcggg agcgacgaca acccataggg aagatgccca gtttaacgcc
    15301 ctatactatt ttttttactt acggtatggt ttgaggcgcc cggaattgcg ccggctttca
    15361 tgcgttcgcg aagcatcatg acacacctct ctcccaaacg gcgatccatg caggcgaccg
    15421 gcctacccgt gctggcgctg gtcatcgccg gcttctggct cctcacgcac cggcgggacg
    15481 gcgtggccga cacgcccgcc aaagcaaccc ccgcagtggt ggcgaccaca gtccgtgcgc
    15541 gacaacagaa cattcccgat tatctatccg gcatcggtac ggtcaccgcc cgccagaccg
    15601 tcaccgtacg cacacgcatc gatgggcaac tcgagaaaat cgattttcgc gaaggccagg
    15661 atgtcaaagc cgggcaactc cttgcccagt tggatccgcg cacgcagcaa gcgcaactgg
    15721 aacaggccaa ggcgcaactg gccaaagacc aagccctgct ggccaacgcc gagctcgacc
    15781 tgcgccgcta ccagaaactg atcacgctca atgccacgtc gcgccagatc ctggatgcgc
    15841 agcgctccca agtcgcacag ttgcgcgcgg acatccaggc cgaccaggct catatcagcg
    15901 acgcggcaac ccaactcagc tacacccgca tcacggcacc catcagcggc cgtgtcggcg
    15961 cgcgcctggt cgatcaaggc aatatcgtgc acaccaccga tgcgaacggt ctcgtggtga
    16021 tcaaccagat cgatcccatc accgtcgtgt tcacggttcc aggcgactca ttcctggcca
    16081 tcaaccacgc catggccgcc ggcgaaccgc tggccgtcat cgtgtacccg cgcgacggca
    16141 cacgttcgct gggcgacggc aagctcgtgt tgatgaacaa ccagatcgac accagcaccg
    16201 gcacaatcat gctcaagggg ttgctcccca atccagggca tgacttgtgg cccgggcaat
    16261 atgttgacgt ccggctcgtc ctcggcgaac ggcgtggcgc catcaccgtc ccggcggcgg
    16321 ccgtgcagcg cggacccgac ggactgtatg tctacacggt ggacaagcac gatatcgccc
    16381 acatgcagcc catcaccgtc gcgcataggc agcaggggct cgccatcatc gacaaagggc
    16441 tgcaagccga tcagcgcgtg gtgatcgacg gtcaataccg gctcaagccc ggcacgcgcg
    16501 ttaccgaagc cccccatcgc gcgacggaaa gcacgggttc cgagaacccc cggagcggct
    16561 cgaattgagc atttcagcca atttcatcaa gcggcccatt ggcacgtcgt tgctggccct
    16621 ggccttgctg ctggccggga tggtggcatg gcgactgttg ccggtggcgc cgctgcccca
    16681 ggtggatttc cccaccatac tggtctcggc cagcctccct ggcggcaacc cggaaaccat
    16741 ggcatccaca gtggcgcagc cgctggaacg gcaattctca gagatcccgg gtctggatca
    16801 gatgacatcg atcagtgcgc tgggtacgac gcagatcacg ctgcaattca atctgaaccg
    16861 caatatcgac gcggccgcgc tggacatcca ggccgccatc aacgccgccg ccggacaact
    16921 gccgcacgat cttcccagcc cgccaagctt ccgcaagatc aatccagccg actggtccat
    16981 cctgatcctc tcggtgcagt ccagagccat gccactgatc gaggtcgaca attatgcgga
    17041 caatctgctg gcacagcaga tttcgcgcat tcaaggcgtg ggccaggtca acatcttcgg
    17101 tgagcagaag cctgccatcc gcatccaggt ggacccgcgg aagctcgcgt ccatggggct
    17161 cacgctcgag gatatccggg gggtgatcgc cgaaaccacc gtcagtcagc ccaaaggcac
    17221 actggacgga gaccatcaga gcttcaccgt ctacaccaac gaccaactaa cagaagcggc
    17281 aaagtggaac gacaccatcc tcgcctatcg gcatggcgcc cccatacgcg tgcgggacat
    17341 cggccgcgcc attcccggcc ccgaggacgt gaagcaagcc gcctgggctt tcgccggcaa
    17401 ggccgcgccc aagactgatg cgcccatcaa cggacgctcg ctcatcatcg gcatccacaa
    17461 ggagcccggc gccaacgtgc tcgaaaccgt ccggcgcatc aaggcggcgc tgccccgcat
    17521 cgaggcctcg ctgcccgcat ccgtgcatgt cagcacgctg atcgaccgga cgcagaacat
    17581 caaggcctcg gtgttcgacg ttgaaatgac gctgctgctg tccatcgccc tggtcacgct
    17641 ggtcatcttc atgttcctgc gcagcgtccc ggccaccctg attccagcgg tcactgtgcc
    17701 catggcgatc ctgggggccg cggtcgtgat gtacctgctg ggctacagcc tggacaatct
    17761 ttcgctgatg gccctgacca tttccgtggg ttttgtggtg gacgacgcca tcgtcatgct
    17821 ggagaacatc taccgacata tcgaagaagg catgtcgccg cttgaagccg cctacaaggg
    17881 cgcgggtgaa atcggcttca ccatcgtgtc gatttccgta tcgctggtgg ccgccttcat
    17941 tccgctgctg ctcatgggtg gaatcgttgg tcggctgttt cgtgaattcg cggtgaccgt
    18001 cgccctgacc atcctggtgt cggtcatcgt gtcgctcacg ctcacgccca tgctgtgttc
    18061 acgcttcatc cggcattatg accgcgcttc ccacggcagg gtgtatctgg ttttcgagcg
    18121 cggcttcgac catatgctcg caggctatcg gcgcggcctg cactgggtgc tgcgccaccc
    18181 gtttctcaca ctgtgctcgt tcctggccac gctcctggcc accgtcgcgc tgttcatcac
    18241 gattcccaaa ggattctttc cccagcagga caccggctat atccatggct tcgccgaagg
    18301 cccgcaagac acgtcctttg gcgcgatgtc ccggcgcatg gaggcactcg ccgatgtggt
    18361 tcggcaggac cccgacgtgg cgacattcgg aatgagcgcc ggccgcacca cattcaacac
    18421 cggcaccttc ttcatcggct tgaggccaaa ggaccagggc cgcacagtca gcgcgaacca
    18481 gatcatcgac cggctgcggc ccaagctcga gaaggtcgaa ggcgcacggt tgttccttca
    18541 agccggccag gatatcaacg tgggcgggcg actctcgcgc acgcaatatc aatacacgtt
    18601 gaccgacgcc aacctagacg agctcaacac ctgggcccca cggctgcttg cgcgttttcg
    18661 acaattgccc gagctcaccg acgtggcgac ggaccagcag aaccaagccg ccacagctgt
    18721 ggtgacgatc gatcgcgcgc gcgcctcgac cttcggcatc acgccggcca tgatcgactc
    18781 caccatctat gacgccatcg gccaacgcca ggtggcgcag ttcttcaccc agctcaacag
    18841 ctacgaggtg gtgctggagg ccacgccgca actgcagaaa gactccgatc tgttccgcac
    18901 gctgtatgtc cgatcgccga tctccggaga cctggttccg ctctcgagtt tcgtgacggt
    18961 gaacacatcg aagaccggct atctgtcgat caaccaccag ggacagttcc cggcggtcac
    19021 cctgtcgttc aatctggcgc cgggcgtagc actgggtcag gccgtcgacg ccatccggca
    19081 cgcccgggcc gacatgaaca tcccgcagac actcagcggg tcgttccagg gcaccgccaa
    19141 ggcgttcggc gaatcgctgg ccacgcagcc ctacctgatc gccgcagccc tgatctcggt
    19201 ctacatcgtg ctggggctgc tgtacgagag ctacatcc
//
LOCUS       DASTNK010000012        57269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_563660, whole genome shotgun sequence.
ACCESSION   DASTNK010000012 DASTNK010000000
VERSION     DASTNK010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 57269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57269
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563660"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VFR20_00780"
     CDS             <1..1128
                     /locus_tag="VFR20_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEU0229079.1"
                     /translation="VFSKVIRFFPPLVTGSVITTIGLTLMPVAAQWAMGGNPHAPDFG
                     SMGNIGLAALTLLIVLLLSKLGSAAISRLSILLAMIIGTVIALFTGQADFSQVSHGPT
                     LAVPIPFHFGFPTFHAAAIVSMFIVIIVTMVETSADILAVGDIIDAKIDSHRLGNGLR
                     ADMLSSMIAPIFGAFTQSAFAQNVGLVAVTGIKSRFAVSFGGLILIGLGLLPVMGRVV
                     ASVPTAVLGGAGVVLFGTVAASGIRALARVEYRNNMNLVIVATSISAGVIPIVAPTFY
                     NHFPAWFATIFHSGISSAAVVAILLNLAFNHISLGNSDQQSVFVAGTTRILRDTDLAC
                     LNDGDRMVDGKLYDKDGNEVPVVCNAHAADHAPRQAAVAAG"
     gene            1181..2077
                     /locus_tag="VFR20_00785"
     CDS             1181..2077
                     /locus_tag="VFR20_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0229080.1"
                     /translation="MDFRHLRQFVVLAETLSFHRAAKKLHISQPPLSVSIRKLETELG
                     VTLFTRGKDGVTLTESGKAALADARRALFHAAQFQQAARAASTGDGGTLRVGFVGSAI
                     HFVLPKILSRFRQSHPGVQLALQEATSLRIMQMLEDESLDVGVVRVPVSSHAGTLMRS
                     LQTEHYDLALPKGHTLAGPAPVRLRDLADEGFVLYAEPNAAGLRMAAIHACQLCGFTP
                     RVVQEAVQVQTVLSLVEAGLGVALVPSINRRFASSQVVYKTLSDYPASASIGISLAWN
                     PDAETAAVRNLRDIAERLFPGA"
     gene            complement(2144..3376)
                     /locus_tag="VFR20_00790"
     CDS             complement(2144..3376)
                     /locus_tag="VFR20_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEU0229081.1"
                     /translation="MNQPQDVEHSSSPTPLPLDGIKVLDLSRVLAGPWCTQTLADLGA
                     DVWKIEAPGHGDDTRIWTPPDVDGESTYFLCTNRGKRSAAVNLRHPQGQALVRSLAGK
                     ADVLVENYRLGALKKFGLDYETLSQTNPGLVYCSISGYGRTGPRAAEPGYDFVIQAES
                     GLMSITGDEEGEPMKLGVAITDLVTGMNAGQAVLAALLVRQRTGRGQLIDLALLDGAL
                     NVLANVGMGYLAAGHIPERFGNAHPTVVPYQIVSASDGRFALAVGNDTQFMSLCAALG
                     RGELAVDERYRTNRARVLNRATLLPELEKTLATDTRANWLEKIRAAGVPAGSVRTVPQ
                     ALDAPEVKARGLVADTPDSRHGSLPIMRSPLALRGTPPREPVAPPRLGEHTDQILREV
                     LGATVDELAAWRDAGAIT"
     gene            complement(3373..4398)
                     /locus_tag="VFR20_00795"
     CDS             complement(3373..4398)
                     /locus_tag="VFR20_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEU0229082.1"
                     /translation="MENPFVEPVERLFAEVAVPVAVRDVEEGGSPDTIWRAVEASGFL
                     DALVPESAGGAGLGLSDAFPLLMLAGRHAVPVPFVQTLLARAWLAAAGVPAPDGPIAL
                     AGVGTTRKAGAVEARNIGFARVAAWVLADLDGGTMLLPVSGATIEPTGGRGSLDATLR
                     WPAQPDGAREVPALPAGSLLRLRDIEAAAWAALIAGAADRALSLTLDYAGQRTQFGKA
                     IGRFQAVQNQISVMAEQAWAARMAAQMACHGPGWQPRPLLAALGKSRCSEAAPLIADI
                     AHAVHGAIGITDEYDLQLYTRRLRAWRLIAGSESYWATVIGEACLRQPGQSALSFIVD
                     QLSAEQA"
     gene            complement(4491..5642)
                     /locus_tag="VFR20_00800"
     CDS             complement(4491..5642)
                     /locus_tag="VFR20_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0229083.1"
                     /translation="MIDTLELAGIPPQDEALRPAIRAFLEEAVGSLAPEVRARSWMGF
                     DAAFSRALADKGWLGLTLPVEYGGAGKGPFARFVLAEELLGAGAPVSAHWIADRQSGP
                     LILKYGTEAQKRFYLPRICRAEAFFCIGMSEPGSGSDLASIRTRATRTDDGWVLNGSK
                     IWTTNAQRSHYMIALVRTSGEPADRHKGLSQVIVDLSLPGVTVRPIRDVAGDAHFSEV
                     FFDNVALAPEALIGDEGQGWAQVNAELAFERSGPERVYSSMVLLEGWLTHCRALGAEG
                     LRHAAPLGRILASLAVLRAMSIAVTAKLARGENPALEATLVKDLGTEAEQMIPRVVGD
                     ILAASPDVPMPASLGRTLAYLEQMAPSFSLRGGTREILRGILARGLGLR"
     gene            6019..6813
                     /locus_tag="VFR20_00805"
     CDS             6019..6813
                     /locus_tag="VFR20_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEU0229084.1"
                     /translation="MTSLLNFDQDDQGVVTLTMNDDATRNALTGSAMVDEFLAAFDRI
                     QADHHVGAVIITGNGKAFSSGGNIDEMGRQTRPEYTPAALRHEYRQGIQRLPLAMQAL
                     EVPTIAAVNGPAIGAGCDLACMCDIRIASEHALFAESFVKLGLIAGDGGSWFLPRLIG
                     MSRAAEMTFTGDAVDARQALEWGLVSRVVTADALMDEARGLAHRITANPADALRMSKR
                     LLRESQIGRLDTVLEMAAAFQSLAHKSDDHARAVRDFLERRAARKK"
     gene            6918..7790
                     /locus_tag="VFR20_00810"
     CDS             6918..7790
                     /locus_tag="VFR20_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0229085.1"
                     /translation="MTTQQPAAAAGQFLIGGDLSINRLGFGAMRITGHGIWGDPADPA
                     QARATLARAPELGINFIDTADSYGPTISEDLIREVLHPYPGMAIATKGGLVRHGPDIW
                     MPLGRPEYLRLCVLMSLRRLGVERIDLWQLHRIDPKVPETEQFEVIAEMHKEGLIRHV
                     GLSEVNVTQIEAAQKHFPVATVQNRYNLVDRQSEDVLDYCERHGIGFIPWYPLAAGAL
                     TRPDSAATRVADRLKITHGQLALAWLLKRSPVILPIPGTSQVAHLEQNTAAATITLSD
                     EDFDAVDRQGREAG"
     gene            7852..8082
                     /locus_tag="VFR20_00815"
     CDS             7852..8082
                     /locus_tag="VFR20_00815"
                     /inference="COORDINATES: protein motif:HMM:NF021321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HEU0229086.1"
                     /translation="MPSGTPSPPKKPAQDPRPLAPRRPGNDECCGNGCIPCIFDIYED
                     QLERYREQLAAWEKRQRRPPRATTCRAHAPDS"
     gene            8125..9033
                     /locus_tag="VFR20_00820"
     CDS             8125..9033
                     /locus_tag="VFR20_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0229087.1"
                     /translation="MIDFKQIEAFVWVAELGGFRAAAEKLNTTQPAISQRIAAMEHSM
                     HVRLFERGARGIKLTEKGQELLSHAQRILDMRSEMLRVAQEENTIRGMLRLGVAETLV
                     QTWLPRLIEELHERYPALIVEIHVDTSHFLRSQLASYQIDLALLVGTSQDPKEVHMHL
                     CDYKLAWIASPALKLHEHTATISELGAYPVITYPSLSLPYHAVKDALTQAGIKTPRIY
                     CCASLSTMVHLTRYGIGPSVIAPAAITDELASGKIRLLDTTVPLPDLRFYACWRDSPD
                     SHTARTVARLARRIALKAKREDRRHA"
     gene            9278..10336
                     /locus_tag="VFR20_00825"
     CDS             9278..10336
                     /locus_tag="VFR20_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEU0229088.1"
                     /translation="MNRFILSTLAAGALFVAGSASALDKVPNLPETHLQVVGGLSNLT
                     AYRNFEVPFWTKTVPELSDGRVTATIKGFNEMGLKGPEIMRLISQGVIPIGTATLAYF
                     ASDNAINEAIDLAGLSPSAKIAREVTNAFTPAYDAYYKKQGVKVLGISTYPAQVLFCN
                     AKISSLADLKGKKVRTSSRTTAEFVQALGGTSVTMAFGDVVPALQNGVVDCGITGSMS
                     GYSAKWYEVTKYLYALPINWNQQIHAANLAYWDKLDPKVQAFLQKGIDHMVDQIWDAA
                     AKETQEGYDCNTGSDKCPLPVKGKMKLVEPTAADRELLKKIMTDVVVPKWAARCSAQC
                     VKDFNDTVGKLRGITAKK"
     gene            10416..11036
                     /locus_tag="VFR20_00830"
     CDS             10416..11036
                     /locus_tag="VFR20_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEU0229089.1"
                     /translation="MNNQDRQAGIGRLLTLARQLSRVAMWAAGSLTLLSALYISVDVI
                     CRKFFNISLGGSDELSGYAFAISISWALSFATLERANVRIDALYQTLPARLCALLDWI
                     SLVCLAVFIVWLTRYAVDVAATSWVRHSTANTVLATPLWIPQYLWAAGLIWLCVVLAL
                     MLLRSSIALVTGDLATIRAICGIRTTAEEASEEAAAGVRMVRGDAA"
     gene            11033..12316
                     /locus_tag="VFR20_00835"
     CDS             11033..12316
                     /locus_tag="VFR20_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEU0229090.1"
                     /translation="MITTALVLLLVLLGLSIPVGAALGALGLILDPLYSMLPLSRAIG
                     EISWSTSNEFLLVAIPLFIMLGEILLRSGLAEKMYNSMSLWLSWLPGGLMHANIGASA
                     LFAATSGSSVATAATVGTVAIPQIAKKGYNESLFLGSLAAGGTLGILIPPSINLVIYG
                     VLTNTSVPKLYLAGIIPGIGMAALFMLAIGAACVLRPKWGGQKISATWGQRMASLKHL
                     VPPLFIFLLVVGSIYAGLATPTEAAALGVVGALILAAWERKLSFTMLRDVLEGTMKAT
                     AMIMLIVLGAAFLNFIMSGTGLTTAVTNTVAHLGLSKSWMLLIMVAFYLVLGCFMETL
                     SMMITTIPIVAPIMIALGFDPVWLGVVIIILVEAALITPPVGLNLFVIQSLRTRGSMN
                     DVLAGSLPFVIMLFVMIGLLALFPNLALWLPHAFG"
     gene            12367..13050
                     /locus_tag="VFR20_00840"
     CDS             12367..13050
                     /locus_tag="VFR20_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 domain-containing protein"
                     /protein_id="HEU0229091.1"
                     /translation="MKLSFELSSATRPETVEVDFQQCIVAGWAGRDLAATEHHIEELA
                     LLGVTRPSSVPLFYRIASNQLVQDPIIDVVGEETSGEAEVFLFSHQGETLVSLSSDHT
                     DRKLEAHSVALSKQICAKPLARHAWRHAEVADHWDDLILRSWIKEDGNPVLYQEGNLG
                     TLLPPLELAQRFFGSIRVPEGSGMTCGTVSTIGAIRPASGLKMELFDPKLGRSITHAY
                     TLCVLPEIA"
     gene            13143..14498
                     /locus_tag="VFR20_00845"
     CDS             13143..14498
                     /locus_tag="VFR20_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU0229092.1"
                     /translation="MLPTLLSLQNDLAEGRTTSVALTEAALARIDDTQGEGTRAFTKV
                     YREQALAAAQASDLLRAAGLVRSPVDGLPISIKDLFDVAGDTTPAGSKVLEGAEPAHE
                     NAVIVQRLLSAGAVIVGRTNMSEFAFSGLGLNPHYGTPSSPWDRATGRIPGGSSSGAG
                     VSVADGMAAAAIGTDTGGSVRIPSAFCGLTGFKPTAARVPTQGALPLSWTLDSIGPLA
                     ASVACCAILDALLSGTVYRAPVAPPLDHLRLASISAISLAGADEHVTQTYRSTLDALE
                     SQGAVIEEISLPEFGELPHINHLGGFAGAESWAWHRQLLAEHAAEYDPRVSARIARGQ
                     AMNAADYIDLQRLRPQWITAVETRLCGYDALLMPTVPVTAPAIADVAASDDAYFAANG
                     LILRNSTYINFLNGCALSLPCHAAGTAPVGLMVAGCANTDAHILGVGQALEGVLARIR
                     G"
     gene            complement(14530..15204)
                     /locus_tag="VFR20_00850"
     CDS             complement(14530..15204)
                     /locus_tag="VFR20_00850"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0229093.1"
                     /translation="MDDWSGISAVDLGKTASAADVIYGALRDAIIRGQLPEGETLRQD
                     NIAQMFNVSRIPVREALKKLESQGLATSVRYKGVVVASMSVDEISEIFEFRALVEPRV
                     IELAVPKLSAESLEEAQRCCDAFSAETRPERWGDINRQFHAALYADANRPYYLSIVNA
                     TSDRVERYIRAQLDLTHGKQRARVEHQAIMDACLRRDAKAAAHLTRDHIVNAGKSLVA
                     FLKEKA"
     gene            15492..16400
                     /locus_tag="VFR20_00855"
     CDS             15492..16400
                     /locus_tag="VFR20_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0229094.1"
                     /translation="MWTGVLPAVTTKFTQDGALDHAEMERCFGLLMEAGCDGLIVAGS
                     LGEGPMLTHQERLDVLKTAQNVAGKRPVLMTVAAASTQESCDLAGKAAKAGASGLMVV
                     PSAIYHTNEVETEATLRAVAAAGDLPVMIYSNRIAYRVDVTPEIMERLADDKHFVAVK
                     ESSDDIRRTIEIFNRVGDRYNVLTGVDNLAFEALSVGAVGWVAGLVVAFPKETVAIYR
                     LMKAGRRDEAMAIYRWFRPLLDLDVSTYLVQNIKLAEALAIDSNDRVRLPRMPLQGER
                     RAQVQAIVEKALATRPQLPDYLKLAA"
     gene            16473..17510
                     /locus_tag="VFR20_00860"
     CDS             16473..17510
                     /locus_tag="VFR20_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEU0229095.1"
                     /translation="MQSSKVIHVVSCHAEGEVGDVIVGGVAPPPGATLWEQSRWLHHD
                     KSLRDFVLNEPRGGVFRHVNLLVPPKNPRAVMGYLIMEPVHTPPMSGSNSLCVATVLL
                     DTGIVPMTEPETRFTMEAPGGLVDVVAQCRHGKAVSVKVVNHPSFADRLDAVIEVAGL
                     GSLRVDTAYGGDSFVLADAAALGFALTPDEAADLAATGMRITRAANEQLGFIHPTNPD
                     WRHISFCQLTLPVTIVDGIATGRNTVVIEPGKLDRSPTGTGCSARMAILHARGQLKEG
                     DAFTGLSILDSRFDCRIEGLTSLSGKAAIIPSIAGHAWITGTHQHLLDPSDPWPRGYR
                     LSDTWPNPDQR"
     gene            17772..18920
                     /gene="argE"
                     /locus_tag="VFR20_00865"
     CDS             17772..18920
                     /gene="argE"
                     /locus_tag="VFR20_00865"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEU0229096.1"
                     /translation="MKQQSLDMITRLVGFDTVSRNSNLALIDYVRDYLAGHGVQSHLV
                     HSPKGDKANLFATIGPNVEGGVVLSGHTDVVPVDGQPWDTDPFTVTEKDGRLYGRGTS
                     DMKSYLAIALAMVPDMLSRDLKRPVHLALSYDEEIGCVGAPSMIDRMAADLPRPGAVI
                     VGEPTSMRPIVAHKGVAALHTTVIGHEAHSSQVQRGVSAVMIAARLITFIDDMMAENK
                     AAADPQCPFVPPYTTLHVGVVHGGTALNIISRECSFTWDIRAMPGDDWHKYLERFNAY
                     AESLLPAMRAVSPDVSITTQAFADSPPLQDQGGAAQELVFKLTGCTCGDVVPYGAEGG
                     QFQERGFPVVLCGPGSIDQAHKPNEYIDISQISACEAFFGQLLDRLAH"
     gene            19186..20790
                     /locus_tag="VFR20_00870"
     CDS             19186..20790
                     /locus_tag="VFR20_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0229097.1"
                     /translation="MQISRFIKHTALASALALGLGLGHAATAKTFRWSYQGDVNSLDP
                     MSLNETFTLGFQNSFYEPLVTYDKDLKLVPALAVKWENPSPTKWIFHLRKGVKFHDGE
                     PFTADDVVFSWKRSLTPGSDMKGYGSLATDVKKLDDYTVEVTTPTPNPILPRDWVFLY
                     MMSKSWAEKHHTTDATNVKGGGDNYANMHEDGTGPFEVTSRQPDVKTTLKRFPGYWNK
                     NIPTNVTDIVFQPIKQESTRVAALISGDMDLVRPIPVQDWPRLEKVPNIELLRKPEAR
                     AIFIGFDQHRKELLYSNVKGKNPFKDKRVREAIDLTVDTNLINKKIMRGAAHPLGTLI
                     ATSINGYDPSFGKPYKVDIPKAKKLMAEAGYPNGFEVTLDCPNDRYVNDEKICQAVAS
                     MLARIGIKIDLLAQTKSKYFGKVLLQAGNKTSMYLLGWTPSSIDAENSLQNLVACRNA
                     KTSAGQFNLGGYCNPKIDDLIAKIGVETDQAKRDAMIKQAFMLLRNDYGYLPLHQQPL
                     SWGVRKGVKVEQRADDVLDMRYVVMP"
     gene            20884..21855
                     /locus_tag="VFR20_00875"
     CDS             20884..21855
                     /locus_tag="VFR20_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0229098.1"
                     /translation="MLNFIIRRIIQSIFVMLAVAFIAFSMFRYVGDPINNMVGQDTTM
                     AQRAQLKKALGLDDPFIVQYARFIRNAAHGDFGVSYRQRVPVRALIASRMPATLELSF
                     ASAIIALFLGIPMGIYTALKRRGVLSQSFMTISLIGISLPTFLTGILMILVFGVLLRW
                     LPTFGRGEVVHIGWWSSGFFTKSGWESIIMPAFTLALFQMTLIMRLVRAEMLEVLQTD
                     FIKFARARGLPERLINFRHALKNTLVPVITITGLQLGSIIAFSIITETVFQWPGMGLL
                     FIQSIEFVDIPVMAAYLLMIGFFFVIINLVVDLLYFAVDPRLRVQKG"
     gene            21891..22808
                     /locus_tag="VFR20_00880"
     CDS             21891..22808
                     /locus_tag="VFR20_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0229099.1"
                     /translation="MTTTTLSRIRNSDLYYSFTHSPAAMISALIALLIFLGAVFAPLV
                     APHNPFDLASVNLMNANMPPAWSPDGSHAFLLGTDDQGRGILSTILYGSRLSLEVGFA
                     SVFFSLIIGVTLGLISGYVGGRIDGFIMRIADVQLSFPAILIALLVDGVARGLLPRDM
                     QDKMAIYVLIFAIGISGWVQYARTVRGATMVERNKEYVQAARLIGMRPFTVLRRHILP
                     NVLGPVLVIATIHLAVAIITEATLSFLGVGISPTTPSLGTLIRIGSNYLFSGEWWITI
                     FPGAALVLLALSVNLLGDWLRDALNPKLR"
     gene            22825..24564
                     /locus_tag="VFR20_00885"
     CDS             22825..24564
                     /locus_tag="VFR20_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0229100.1"
                     /translation="MALLDIENLKIEFPSRRGDLVAIDGVSLALEKGEILGVVGESGA
                     GKSTIGNAVIGLLQAPGRMTGGTISLQGKRLDELKPHELRLVRGRRIGMIFQDPLTSL
                     DPLQTIERQLVETMQVHLHLSAAEARRRAIDILDSVGIEDAAVRLQQYPHQFSGGMRQ
                     RVVIALALCCEPEVIIADEPTTALDVSIQAQILELLRKLCKEKQVGMIIITHDMGVIA
                     EITDRVAVLYRGKLVEEGTTAKILGSPDHPYTKSLISAVPRSDVKLRRFPMVTYIEDV
                     LAPRKRELDISTHWLGQERDFGKHEDTALLDVQDLSMRFILRNAFLKKNRLTFDAVKH
                     VNFSIREGEVFGLVGESGSGKSTIARLIAGLYKANNGSIIFDGTNVSSLKDERQLNAF
                     RRQIQMVFQDPFSSLNPRMRILDIVAEPIRFHRLTTSEAQTREIVSDLLDYVGLGDQA
                     LRRFPHEFSGGQRQRICIARALATRPRFLICDEPTSALDVSIQAQILNLLKDLQEKLH
                     LTILFISHDLPVIRQMCDRVGVLRHGELLEVAETEALFSNPRHEYSQHLLSLMPTMHF
                     MSREGLELEASAA"
     gene            24739..24987
                     /locus_tag="VFR20_00890"
     CDS             24739..24987
                     /locus_tag="VFR20_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU0229101.1"
                     /translation="MHIIGMIIVGFIIGLIARAIMPGDQKMGLIMTTILGIVGSLVAG
                     YIGQALHWYAPGQGAGWIGSIIGAIILLFIYGMVKKNQ"
     gene            25213..26637
                     /locus_tag="VFR20_00895"
     CDS             25213..26637
                     /locus_tag="VFR20_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEU0229102.1"
                     /translation="MKPDGAHFTDYWLAEWLRLREAHWGPLEDAREVRQARAQGRTFA
                     QRIVLRARFLCEREKLDTVPARWWQGARLILPALGLLAILAGSGAALGALGNGIVPVN
                     IALALTAMLGLHCLTYLLWLASFALHGHAEGGAQLGRLWLWLTRKLARGPDAALVPRA
                     LTGLLNRNGSLRWLLGSVSHGLWTVAFTSLLLTMLAVLSARRYDFGWQTTLLTPDTFV
                     HLAALLGWAPAHLGFATPPADIVRASNGLHTLPDTARALWSNWLMGCVVVYGLLPRLL
                     GLVLCLAMTRYRIRRLDLDVTLPGYADLRQRLVPSSEVTGIDKPDTDIATGPGFATHP
                     SSPIAGRPAIAGIELPHDQPWPPVSLDARIVDLGVVDSRPQRQATLERLQHSPPSRLL
                     VVCDARQTPDRGAVALIAELAADAGATMVALTLPANDTPTPQHRAGQWRDRLVDAGLA
                     SDLIVEDTHQALSWLTTPPGASNE"
     gene            26630..28051
                     /locus_tag="VFR20_00900"
     CDS             26630..28051
                     /locus_tag="VFR20_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HEU0229103.1"
                     /translation="MSDTLPLTLAVVGHTNTGKTSLLRTLTRNPDFGRVDDQPGTTRH
                     VEGTRLLIDDQPVVELFDTPGLEDSMALLDYLDQIAERAQRLDGPDRIRRFLDSPESK
                     RRFEQEARVLNKLLACDAGLYVVDVRDPVLSKHKDELTLLASCGRPLLPVLNFIHSPG
                     EHSQVWRDTLARLGLHASVEFDTIAPALDGEDQLYGKLALLMDAHAALLSELRDDVSR
                     QRLIRRADAARLIAELLVDAAAWHVGCAPDDESVRQATDTLRRKIRRREADCVKALLK
                     RYNFRTADFPQHALPLQGERWGMDLFHPQALKDAGLHVGKGIAAGAMAGVTVDVFTAG
                     LSMGAAALVGAALGGLWQGTDRLGKRIMGRLKGFRELSVDDAVLRLLQVRQLALVRAL
                     ERRGHAAQSPIVLAADGAPPTMDSSPEETRTVAVARPAPPPLPDELTEARSQPQWSTL
                     DKHYAHSPRRDQIVRALAERLAP"
     gene            complement(28125..28946)
                     /locus_tag="VFR20_00905"
     CDS             complement(28125..28946)
                     /locus_tag="VFR20_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0229104.1"
                     /translation="MRNTFLGKALVRCLLPLLGSLMIATSGTASAADNYMQTIKDRGE
                     IRVGILVDWPPYDGVDADNKPAGYDADVAKLLADYLGLKLKLVVMTGPNRVPFLLTDK
                     ADVLVASLAITPERQKQVQFSQPYSAASMVLLGPDSVHISSPQDFLKYKIGVPRASTT
                     DLGVSKVAPKGTNLRRFDDDASALQAMLVGQIDAVGTSSVIAANVQKRYPGKYKVLYV
                     VNEQQMAIAMQKNRPELLKTMNGFIAANIKNGKLSAAFQKWLGTPLPASVVDAGK"
     gene            complement(29313..30179)
                     /gene="kynA"
                     /locus_tag="VFR20_00910"
     CDS             complement(29313..30179)
                     /gene="kynA"
                     /locus_tag="VFR20_00910"
                     /EC_number="1.13.11.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HEU0229105.1"
                     /translation="MTKESTPGTGPQGVKDEGWYDARLDFSHSMSYGDYLALDAVLTA
                     QHPRSGNHNEMLFIIQHQTSELWMKLALHELHAALEAVRGDDLPPAFKMLARVSRIME
                     QLVQAWSVLATMTPSEYSAMRPSLGSSSGFQSHQYREIEFTLGNKNVAMLKTHAHRPE
                     IAAHLRRSLEAPSFYDEVVRLLARRGFAIDPARLTRDWTQPTRHDATVEAAWLAVYRD
                     PSHHWDLYEMAEELVDLEDAFRQWRFRHVTTVERIIGFKQGTGGTNGVPYLRKMLDVV
                     LFPELWHVRTVL"
     gene            complement(30228..31481)
                     /gene="kynU"
                     /locus_tag="VFR20_00915"
     CDS             complement(30228..31481)
                     /gene="kynU"
                     /locus_tag="VFR20_00915"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEU0229106.1"
                     /translation="MVVNRNDCLDLDRRDALAPLRGQFTLPADVIYLDGNSLGVLPCQ
                     ADERARRIIAEEWGAGLIRSWNDAQWFTLPRRLGDALAPLVGANAGEVVVTDTTSTNL
                     FKVLAAALAMQKHTDPARKVIVSERSNFPTDLYIAEGLMRLLDDGYALRLIDAPSALP
                     DALRDDVAVVLLTHVNYRTGAMMDMAARTADIHRCGALAVWDLCHSVGAVPVDLNGAQ
                     ADFAVGCTYKYLNGGPGSPAFVWVNPRHQNVFEQPLSGWWGHRAPFAMDPGYQPDDGI
                     GRYLCGTQPILSLSLVQCGLDIFSQTDMAAIRAKSLALTDLFIELVESRCAGFPLTLV
                     TPREHAARGSHVSFEHPQGYAVMQALIARGVIGDYREPRIMRFGFTPLYIRYVDVWDA
                     VEALREILASGAWDRPEFQARGAVT"
     gene            complement(31618..32253)
                     /gene="kynB"
                     /locus_tag="VFR20_00920"
     CDS             complement(31618..32253)
                     /gene="kynB"
                     /locus_tag="VFR20_00920"
                     /EC_number="3.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylformamidase"
                     /protein_id="HEU0229107.1"
                     /translation="MNDNRRIWDISPAIGLGTPTWPGDTPVAEERVWHIGPGCPVNVG
                     RVTLSPHTGAHADAPLHYDAEGAPIGAVPLDPYLGPCRVIHCIGAAPVILPSHLHGRL
                     DGTPPRVLLRTYQRAPVERWDSAFCAIAPETIDVLAAHGVRLIGLDTPSLDPEASKTL
                     DAHHRVRDLRMAILEGLVLDAVEEGDYELIALPLKFASLDASPVRAVLRAL"
     gene            complement(32266..33489)
                     /locus_tag="VFR20_00925"
     CDS             complement(32266..33489)
                     /locus_tag="VFR20_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0229108.1"
                     /translation="MSDTGSDNPLWGSGWRDVRRLWSLDSGAAHLNHGSYGAVPIHVR
                     EVQDAFRAEVDSNPERWFRRRMPDEVVKARTAIAGFLGASADAIALVANASAGASTVL
                     ANLGLAPGDEIIITNHTYGAVRFAAQRRCQDAGGALVTVDVRAGAADDEILGTFDKAI
                     TGKTRLIVVDQITSDTAMLFPIAAILALAHSRGVPVLVDGAHAPGMLDVDLDALDPDF
                     WVGNLHKWCCAPHGTAVLYVAEPFRAGFRTLAVGTKDGEAFPRNFDYTGTADFTAWLA
                     APAALALLGGLGWDKVRRHNAALCAAGQHAVAAELGIPERELWGSPRLSMRALPLPPE
                     LVSSADGARALQARLAHEFALETVIKVWGGAGLIRLSAHVYNALPEYEKLAHVLGTLA
                     HSPKVSSINPVRSMF"
     gene            complement(33553..34353)
                     /locus_tag="VFR20_00930"
     CDS             complement(33553..34353)
                     /locus_tag="VFR20_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU0229109.1"
                     /translation="MTTYDTLVKDIGPLGKRKLRRLSMVIALVIAAGVALLVYGLYAK
                     GELAPSKWALFGVWPTWRFLLGGLVNTLKVAVVDAVIAISIGALVALGQVSTHKCLRW
                     PSVLYAEGFRSLPTLLLVLFAYFGLPPLGFEISAFWALVLGSAAYNSAAFSNIFKAGI
                     KSLGRGQFQAAASLGMNYWVRMRFVIWPQAFRRMLPSTIAQFVVLLKDSSLGFFIGYE
                     ELLRQSEVIGAYTNNFLQSYLVVGVVYILLCYGLRRLALHVGDIQSGE"
     gene            complement(34350..34997)
                     /locus_tag="VFR20_00935"
     CDS             complement(34350..34997)
                     /locus_tag="VFR20_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU0229110.1"
                     /translation="MTNVIIGNLDAILHGILITLELSAVCIVGAGILGLIVGVMRVSP
                     VGALQKLATFYIEVLRSTPIPVIFVIIFFALPDMGISLSPFVSASVGLSMYMASYVAE
                     AVRSGVNSVAVGHIEAARAIGLTQLEVLRFVVLPQAVRSVIPTLGNLSVDLIKDTSIA
                     YTISVMEITGTATTLISKFAQPIPLFLAAAVCYLVLTVPTSVFFRRLEKTHTIIR"
     gene            complement(34990..35904)
                     /locus_tag="VFR20_00940"
     CDS             complement(34990..35904)
                     /locus_tag="VFR20_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0229111.1"
                     /translation="MKHILWRGPSRAVRLSAVLVALSCSGLIGMGVAYGASTGAAPTF
                     PKDSTMAKIQARGKLVVGVKYDVPTFGQKNPITGAVEGFEPELAKILGRDMFGADGHV
                     EFVEAPGPSRETIVQQGRADLVSSTYTITPEREKAISFAGPIYVSTPAIMVRKDYKGL
                     GDSIPGYQALNGHSVCAVTNSIESNLLSKKAPNAKIIHLTSNAQCVKALEQGRVDTYA
                     TDDVIIFGFLKKEPHKFRFVANPNGEKQPYGIGVKKSSKDLCEWVNKELLVAFQDGSW
                     KKAFADTIGKVVSTIPEPPTAADMHYCD"
     gene            complement(35955..36746)
                     /locus_tag="VFR20_00945"
     CDS             complement(35955..36746)
                     /locus_tag="VFR20_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU0229112.1"
                     /translation="MDTPASADDRALVVSVRHATKRFGAVAALTDINLDVYRGEVVAI
                     LGPSGSGKSTLCRCINRLEHIDEGTIHIEGTEIPREGKDLALLRADLGMVFQSFNLFP
                     HMTVLENVILGPVRVRKQTPAQATELALRLLAKTGIDGLADKYPANLSGGQQQRAAIA
                     RALAMGPRLMLFDEPTSALDPELVQGILDIMKALAADGMTMILVTHEVHFAREVADRI
                     VFMVDGRIVEEGTPAQFFTQPKTERAARFLSQIMSYPVASFERHG"
     gene            complement(36761..37270)
                     /locus_tag="VFR20_00950"
     CDS             complement(36761..37270)
                     /locus_tag="VFR20_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEU0229113.1"
                     /translation="MRHDGGKWKEWTGWAFVEGHWLRRKHFTKPFLDEVARRIAEGER
                     AHPGELMLAIEAVSPGHERDTRLRALEVFGRLRVWDTPGNSGVLLYMALDRHSIELVA
                     DRGVPVPDDQWAQVCANLRTDLQFGRYTDGVLAAIDAIEAKLAEHCPRLPGVQGGGIN
                     ALDDRPVLL"
     gene            complement(37425..38294)
                     /locus_tag="VFR20_00955"
     CDS             complement(37425..38294)
                     /locus_tag="VFR20_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HEU0229114.1"
                     /translation="MALCLALLASFAQVARADEAKVPVLTQRVTDLTHTLSADTVTRL
                     TATLAGLEKKKGAQIAVLMVPSTDGDTIEHYARRVFDQWKLGRKNVDDGILFVIAKKD
                     HTMRIEVGYGLEGAVPDILAGRIIREQVAPAFRKGDFDQGVSAGVDSLVKLVNGETLP
                     PVKQTARQDTGSAPLPMLLPLVFMAFVLPPAGAAFAAALFVFFMFGSVFLACLGAMVG
                     FVLALAGRAARAGGRRNSLRASRNGAMLGGIVGGWGGGMGGGFGGGGGLGGGGGFGGG
                     GGGSGGGGASGGW"
     gene            complement(38320..38940)
                     /locus_tag="VFR20_00960"
     CDS             complement(38320..38940)
                     /locus_tag="VFR20_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU0229115.1"
                     /translation="MKRFLLFLLLPCLTLMLSGCGYNDIQRLDEQVKASWSQTLNQYQ
                     RRAELVPKLVSAVNAYMVNERTVLEQVTDARSRVGSIQISAKDLDNPALMKKFEAAQG
                     QLSSALSRLLAVSENYPQLKSDGLFRDLMTQLEGTENRIATERGRYVKAVQAYNLAIR
                     QFPTLVTAKLFGYHTMENYGVDQQDKLMRDPEVKFDIPGAAGAGAK"
     gene            39381..40898
                     /locus_tag="VFR20_00965"
     CDS             39381..40898
                     /locus_tag="VFR20_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU0229116.1"
                     /translation="MNTHVSFASLGLAEPLLRAVADTGYTHPTPIQAQAIPQVMKGGD
                     LLAAAQTGTGKTAGFTLPVLHRLTQSTPAALRPGRPRCLILTPTRELAAQVEESVRTY
                     GKHTSVRSLVMFGGVNINPQISALRKPVDILVATPGRLLDHVQQKTLDLSGVEILVLD
                     EADRMLDMGFIRDIRKVLALLPKTRQNLLFSATFSDDIRTLSRTVLNNPEEISVARCN
                     TTSDLVSQRVVLTEPGHKRDLLSFIIRESGWHQVLVFTRTKHGANRLAEKLVKDGLVA
                     AAIHGNKSQAARTRALSGFKEGTVAVLVATDIAARGLDIDHLPNVVNFELPNVPEDYV
                     HRIGRTGRAGVEGNALSLVDHTEMKYLSAIEKLIKTSIERTAIEGWTPAMVKAEPPRS
                     DARPAQAPGQRRSPRKPETGGAPTRSGQARRNTGRGDAPARHAEGNARRAEGNPRRAE
                     GSARRTEGSPRHAEGSARRAEGAVRHAPRGADARPRTGAPRRDASGQSRPVLLSK"
     gene            40929..41558
                     /locus_tag="VFR20_00970"
     CDS             40929..41558
                     /locus_tag="VFR20_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEU0229117.1"
                     /translation="MTLSTWLAFFAASWAISFSPGPGAISAMASGLKHGFRRGYWTTI
                     GLILGIICQVIVVGVGLGALIAASESVFSVVKWLGVAYLIYLGWKQFRTDASPVAVES
                     NRASHFSIRELVTHGWLVNITNPKGTVFLLAVVPQFLDLSHPLAAQYLAIGATLCFTD
                     LFAMGFYTALSSRLLKVLRNPRHIRWMNRTFGALFILAGTVLASFSHHR"
     gene            41606..43222
                     /locus_tag="VFR20_00975"
     CDS             41606..43222
                     /locus_tag="VFR20_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU0229118.1"
                     /translation="MSLTHEVERRRTFAIISHPDAGKTTLTEKLLLFAGAIQIAGSVK
                     ARKASRHASSDWMEIEKQRGISVASSVMQMEYRDCVINLLDTPGHQDFSEDTYRVLTA
                     VDAALMVIDAANGVEPQTIRLLQVCRARNTPIITFVNKLDREVREPLDLLSEIESHLG
                     MDAVPFSWPVGMGRTFGGVFDIGHDRMRVFRAGHDRRDDHNEFIEGLDNPEAAQRFGD
                     AFSRARDDIGLLQAAAPVFDQQAFLDGRQTPVFFGSAINNFGVQEVLDALVDLAPRPG
                     PRQALEREVRPEEPKFSGVVFKVQANMDPAHRDRVAFVRVSSGRFERGMRLMVARNGK
                     EIRPNNVVSFLSQRRELLDEAYAGDIIGIPNHGILQLGDALTEGESLHFTGLPFFAPE
                     LFRSVEVKDPLRTKQLRTGLTQLGEEGAIQVFRPEITGGALLLGAVGQLQFEVVAHRL
                     LTEYKVDARLAPSRYTMARWITSKDPKAMRKFTQANAANIAYDVVDAVAFLASSPAQL
                     RVAQELYPDIEFHAMREHGGHVFSQGTAAG"
     gene            43321..43971
                     /locus_tag="VFR20_00980"
     CDS             43321..43971
                     /locus_tag="VFR20_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229119.1"
                     /translation="MSWRFIIAVLLIAAGASAWGGLRLGNWLIAHGPEKAILHNEPAM
                     PKVPVLDANGHAYSAQPPQPLADGQLGYQQPIPPVDWHVQQADAATAQPNLNIAMATT
                     TITMDQAKLIAAGGGSTQGANLSGIADVSGLVGGKPAKAPLQPVDVPPPPPPPDQMPT
                     PNTNRNWQARFHKALEICSKQGFFDRPSCAWAARNQYCEPNHAWGKVRDCPAKKEF"
     gene            complement(44144..44623)
                     /gene="bfr"
                     /locus_tag="VFR20_00985"
     CDS             complement(44144..44623)
                     /gene="bfr"
                     /locus_tag="VFR20_00985"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEU0229120.1"
                     /translation="MKGDKTVIQHLNKQLKNELSAINQYFLHARMLNHWGFDRMGKHE
                     HEESIGEMKHADRLIQRILMLDGLPNLQDLHKLMIGENVPEILACDLKLESAAQDTVK
                     QGMAYCESVHDYVSRDLFQDILNDTEEHIDWLETQIELVGKVGEQNYLQTQIGEGAE"
     gene            complement(45148..45420)
                     /locus_tag="VFR20_00990"
     CDS             complement(45148..45420)
                     /locus_tag="VFR20_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU0229121.1"
                     /translation="MFICVCNAISENQVRSAVTAGARSLSDLQSQLGVGACCGCCSET
                     ATEYLPGGRYAGQVSHETSVAPVVAANDASADTEIVPMVAIAARRA"
     gene            complement(45479..46903)
                     /gene="rpoN"
                     /locus_tag="VFR20_00995"
     CDS             complement(45479..46903)
                     /gene="rpoN"
                     /locus_tag="VFR20_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEU0229122.1"
                     /translation="MQPGPFLELKQRQQLALTPQLQQSIRLLQLSAQDLEAEIAQAVQ
                     ENPLLERQEEYDIEAPQGEEPRDAETEPLYWSTPSVTARRGDGDGDGIVETSADETLS
                     DHLLGQLRLTRATPRDCLLVGILIGELDENGYLPVSLAEVATAFPDVAADADEWRAAV
                     RLLQSFDPAGVGAGALSECLLLQLDARRADASPGVLACARDIARDHLGLLAAGSVNRL
                     CDVLSCSHDTFQAAHALLLRLDPRPGRAWATSVADFVVPDVLVRKVKGGWRVFQNPAL
                     APRLRVNPLYERLLGDGPDPRALQAQVQQAHNLIKGVNQRFSTVLRVTQVIVSRQEAF
                     FERGMGAMRPLLLRDVAQELDMHESTVSRATRQKYAQTPWGVVELKRFFGAALATDDG
                     GTTSGEAVRSLIIKLVSEESGKKPLSDSQIAARLSDQGVVVARRTVAKYREAAGIEAA
                     SVRKARAALCGGDPDTRRVARDCV"
     gene            46919..47461
                     /locus_tag="VFR20_01000"
     CDS             46919..47461
                     /locus_tag="VFR20_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229123.1"
                     /translation="MKQNSPNEPTRQDGALLRNTLLKLTAAQKILCVVVVVVVALVWY
                     DLLNQLVAFGHNIDYSGLHALGNDMVQMLKLYNPYFWWGVVALCTLIIAYFLHLFVQA
                     TRRRVRMRLVSADVIHTLARELSQPAKDVLLWVWQDRHDPITVGDLQRTSDELHARRA
                     DKIRLAQDHASALLGEQNGA"
     gene            47845..49536
                     /gene="leuA"
                     /locus_tag="VFR20_01005"
     CDS             47845..49536
                     /gene="leuA"
                     /locus_tag="VFR20_01005"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU0229124.1"
                     /translation="MIPVPSDKYRPFVPFTRDFSERTWPSKRITHPPIWMSTDLRDGN
                     QSLIEPMNVKRKLRFFEQLVKIGFKEIEVGFPSASQTDFDFVRKLVDDKRIPEDVTVI
                     VLTQAREDLIARTVESVAGAKQAIVHMYNACAPGFRKIVFNMTQDEVKKIATDGTRLV
                     KHYTSQHPETSWRYEYSPEVFSTTEPEFALDVCNAVGKVWQPTPESKIIFNLPATVEA
                     TTPNMYADQIEWMHTHLDNRDCVVLSLHPHNDRGTAVAAAELAIMAGADRVEGCLFGN
                     GERTGNVDLVTLALNLYTQGIHPGLDFSDIDEVRRCVEYCNQLPVHPRHPYVGDLVFT
                     AFSGSHQDAIKKGFARQDPNALWEVPYLPIDPADLGRSYDAVIRVNSQSGKGGVSYLL
                     ESEHGLVLPRRLQIEFSRAIQRVTDETGKEVTAEAVYDIFSTEYLKQEHPLKLVRHRI
                     SGDPKAAAGQQFQIEAEIEENGQVRNLTGKGDGAIAAFVNALKLPIRIMDYSEHAIGT
                     GTDTRAASYVELRIGESPSGFGVGIHGDIVTASFLAILSAVNRHMISQTQEAMAA"
     gene            complement(49706..50053)
                     /locus_tag="VFR20_01010"
     CDS             complement(49706..50053)
                     /locus_tag="VFR20_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HEU0229125.1"
                     /translation="MLVVFYSKAAAEVLMFAKHALPILKAAGKPYTDTLPERGVITRD
                     QLDAAIAGIEHAIAADKEPELSDESDQIASEIHPISQAVSFRRRAFPLLAMLRLSREH
                     NVDVTWEPGPSSW"
     gene            50155..54069
                     /locus_tag="VFR20_01015"
     CDS             50155..54069
                     /locus_tag="VFR20_01015"
                     /inference="COORDINATES: protein motif:HMM:NF021508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HEU0229126.1"
                     /translation="MTRPVRLSIITVCLPVALMVGTPGHGQPTCGPPSLGSPCALGGP
                     ALLPPNEPSLNLGAGNPIHLATGNKHQRETDLPAIAGVPGLEIVRHYNSRDHRQSAFG
                     SGWSLSYDARLYHIGATWQIAQPDGSRILFQGGPDGPMPNRHGRLESHQSGWRWTWPN
                     GRELRFDTHGRLIAIRLAARHALTLARHEGPESLRGAIASVTHNGGAKLTLHYLVRGG
                     HAYVDRVGSPVGVFQYQYEPVPASSLDDGTAPTWRLVGLTRPDGMRRRYLYEPEHQAG
                     DPLLLTGVAIVSADGRRMARTNSWAYDKTGRAIRSTQGGPDATKGHVSITYVRPPSPD
                     RTGLTRVTDADGQRSQFITAVKGGRHVLLDAEGAGCSGCPAPGTHAQYDELGRLTEVN
                     GTRIERDVSGAITSVAPSGMGWPALALHYQANGRRASWTSAVTGTEHTRFDAAHRPVR
                     REFANGDVWEYDYDAHGRPVRLRESSVEATGAPSTASTPGVADAEGTAHPSGMVSPQT
                     TTLSWRGRLLERVTHPHENEWRGYDAGQRMVRRVVERPHGSVPQRHIDTFHYDNHGRL
                     ARHDLPEGGALLYHYAPRGRLLGIDWLDRDARAHTVIATTAGRPGYRYGNGLTLTTRR
                     THGQDTGLAVADAKGVLLAESRRYDNLGRLAQETHTAPAAGFDETWRYAYDARGRMAG
                     ARRFTRAGAVSARGNPNPTDVITGKSGDNANRPPPSRSMASDPSIWYAWRDDGSLAAR
                     RDGGRTQRPTIARDPSGLPRAVGDIVLEYGPNRRLRRAARGASTLATYRHNAFGQRIT
                     AESTLARTDYFYLDNKVVAEAARPVPRTQPSGRATAGTTAPDPPTITRRYIYAGDVLV
                     GLIDHPLDQSATVGTLYAVHTDLLGAPRAVTDASQAIRWLAAYTPTGEARRVAGDLTL
                     DVRLPGQMFDAATGWHDNLLRTYAPALGQYLEPDPLGPVPGNQAFGYAAQRPRRYTDP
                     LGLLLFAFDGTRNSPVTQSNVWKMARQYLDGPVFYQAGPGTPANLDWDAVTARSAPDI
                     LAAQWDALLQTLARPATRNDTIPIDIIGFSRGAALARHFANQIAGNTRDGYFSYTDAE
                     RGLITACVDLRFMGLFDTVAQFGLLGSQNANYDLTIASAWEWVAQAVALQERRRFFPL
                     VSITGQGNTIEAPFIGAHADIGGGVSTDATGQATSRGDLSDVTLNWMLWQARAATARF
                     APPTSEDGEITDPILHDERLPSSRSLDDGDRRVDGPDGKQVVDRQDDLARLGRAPRAA
                     TEALITRTDNWRYLDIAEVGTVDMDGYARWLHDELGWQALPA"
     gene            54278..55963
                     /gene="argS"
                     /locus_tag="VFR20_01020"
     CDS             54278..55963
                     /gene="argS"
                     /locus_tag="VFR20_01020"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU0229127.1"
                     /translation="MLPRQRQQLISLLQTAVDSVVPGAASDVVLEQPKVAAHGDIACN
                     IAMQLARRVGRKPRDIAEAITQALRDQPTLAGLIASIDIAGPGFINFHLTAAARQAVL
                     DDIAEQGPDYGFLPGNDQKVLVEFVSANPTGPLHVGHARQAALGDALCDLFSSQGYAV
                     TREFYYNDAGNQINNLAISVQARARGIAPDAADFPADGYKGDYILDIANDFLAGATVQ
                     GADREAVTASRDIDDLDAIRRFAVAYLRREQDLDLQAFGLRFDNYFLESSLYSSGRVE
                     KTVQALVGAGHTYENDGALWLRTTELGTGDDKDRVMRKSDGSYTYFVPDVAYHVNKWE
                     RGFHRAVNVQGSDHHGTVARVRAGLQGLDQGIPKDYPAYVLHKMVKVVRDGAEVKISK
                     RAGSYVTLRDLIDWVGRDAVRYFLIQRRADSEFVFDIDLALSKSEDNPVYYVQYAHAR
                     ICSMLAQSGVSHDAIAAAGTDVLTAPSEFALLRRLAGFPDMLAQATQELAPHHVAFWL
                     RDCAADFHAWYGAERVLVDDSQLRLARLRLAQATSRVLAGGLRLLGVSAPEKM"
     gene            55973..56725
                     /locus_tag="VFR20_01025"
     CDS             55973..56725
                     /locus_tag="VFR20_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEU0229128.1"
                     /translation="MARKTRKSGKSRGMSGTMMGLVAGLMLGVGAAVAVAFFVLEVPM
                     PFVDKASRAPATTLLPGVRNAPDPNIGLYGPDGPAGTVPAGQGVTVVTPLPGTGSAAN
                     GQASSNALANQGTAAGQNAPVEQNTPHKAKSPRSPDALGSLIASLGKTPVPGTQAEPK
                     TAPHRARTEPAQGTQTVYYLQAGAFHSSKDADAMKARILMLGLSAQVEDASVNGATLH
                     RVRVGPFKGIDAMNRARAKLGDDKIASSVVRH"
     gene            56806..>57269
                     /locus_tag="VFR20_01030"
     CDS             56806..>57269
                     /locus_tag="VFR20_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEU0229129.1"
                     /translation="MSPKSLFLRLFAVIAIGAGTLFAPASQAVTATQPLYATLDPAQT
                     PDTPPGKIEVLEFFAYTCPHCNAIEPLLRKWEKTLPSDVVVHPVPVAFNASMTDLQKL
                     YYSLVAIGRTDLHDKVFAAIHEENKPIYDEKAILDWIATQGVDRAKFKAVFDS"
BASE COUNT        10480 a        19745 c        17309 g         9735 t
ORIGIN      
        1 gtgttctcga aggtgatccg gttcttcccg cccctggtga ccggctccgt gatcaccacc
       61 atcggcctga ccctgatgcc ggtcgccgcg caatgggcca tgggcggcaa cccccacgca
      121 cccgacttcg gcagcatggg caacatcggc ctggccgcgc tgaccctgct gatcgtgctg
      181 ctgctcagca agctggggag cgccgcgatc tcgcgcttgt cgatcctgct cgccatgatc
      241 atcggcaccg tcatcgccct cttcacgggg caggcggatt tctcccaggt cagccacggc
      301 ccgaccctgg ccgtgccgat cccgttccac ttcggtttcc ccacgtttca cgccgcggcc
      361 atcgtttcca tgttcatcgt catcatcgtg acaatggtgg aaacgtcggc cgacatcctg
      421 gccgtgggcg acatcatcga cgccaagatc gactcgcatc gcctgggcaa cggcctgcgc
      481 gccgatatgc tttccagcat gatcgccccg atattcggcg cgttcacgca aagcgcgttc
      541 gcgcagaacg tgggcctggt ggccgtcacc ggcatcaaga gccggttcgc ggtgtcgttc
      601 ggcgggctga tcctgatcgg cctgggtctg ttgccggtga tgggccgcgt ggtggcgtca
      661 gtgcccaccg ccgtgctggg cggtgccggc gtggtgctgt tcggcaccgt ggccgccagc
      721 ggcatccgcg ccctggcccg ggtggaatac cgcaacaaca tgaacctggt catcgtcgcc
      781 acatccatca gcgctggcgt gatccccatc gtcgccccca ccttctacaa ccacttcccc
      841 gcctggttcg cgacgatctt ccactccggc atcagctcgg cggcagtggt ggccatactc
      901 ttgaacctgg cgttcaacca catcagcctc ggcaactccg accagcagtc agtcttcgtg
      961 gccggcacga cgcgcatcct gcgcgacacc gacctggcct gcctgaacga cggcgaccgc
     1021 atggtcgacg gcaagctgta tgacaaggac ggcaacgagg tgccggtggt gtgcaacgcc
     1081 cacgcggcgg atcacgcccc gcgccaggcg gctgtggcgg cgggctgata tatgatcgcg
     1141 atatcgatac cgatatcgcg atttctcttt cctccgcctc atggacttcc gacacctgcg
     1201 gcaattcgtg gtgctcgcgg aaacgctgag cttccaccgc gccgcgaaga agctgcatat
     1261 ctcccagccg cccctttccg tttccatacg caaactggag accgaactgg gcgtgacgct
     1321 cttcacccgg ggcaaagacg gcgtgacgct gacggaaagc ggcaaggcgg cgctcgccga
     1381 cgcgcgccgc gccctgttcc acgccgccca gttccagcag gccgcgcgcg ccgcatccac
     1441 cggcgacggc ggcacactgc gtgtgggctt cgtcggatcg gccatacatt tcgtgcttcc
     1501 gaagatcctc tcgcgattcc gccagagcca ccccggcgtg caactggcgc tgcaggaggc
     1561 cacatccttg cgcatcatgc agatgctgga agatgaaagc ctggatgtcg gcgtggtgcg
     1621 cgtcccggta tcgtcgcatg cgggcacact gatgcggtcg ctgcaaacag aacactatga
     1681 tctggccctg cccaaggggc acacgctggc ggggccggct ccagtgcgcc tccgggatct
     1741 ggccgacgag ggcttcgtcc tgtatgcgga gccgaacgcc gcgggcctgc gcatggcggc
     1801 gatccacgcc tgccagttgt gcggtttcac ccccagggtg gtccaggaag cggtgcaggt
     1861 gcagaccgtc ctgagcttgg tcgaagcagg cctgggggtc gccctggtac cgtccatcaa
     1921 tcgccgattc gccagttcgc aggtggtgta caagactctg tcggattacc cggcgagcgc
     1981 gtcgatcggg atttcactgg catggaatcc ggatgcggag acagcggcgg tgcggaatct
     2041 ccgggacatc gcggagcggt tgtttcctgg cgcgtgatgt ggttgcctga aagccggacc
     2101 aggggcggtt tcgggaggcg ggccaccacg acaacgcagc cccctacgta atcgcccccg
     2161 catccctcca ggcggcgagc tcatccacgg ttgctcccag gacctcacgc aggatctggt
     2221 ccgtatgctc ccccagccgc ggcggcgcaa caggctcacg tggcggcgtc ccccgcaacg
     2281 ccaggggcga gcgcatgatc ggcagactgc catgacgtga atcgggcgta tcagcgacaa
     2341 gaccgcgcgc cttgacctcg ggcgcatcca gcgcctgcgg cacagtgcgc accgaaccag
     2401 ccggcacccc ggcggcgcgg atcttttcca accagttggc gcgcgtatcc gtggccagcg
     2461 tcttctccaa ctccggcagc aacgtggcgc gattcagcac ccgcgcgcgg ttggtgcggt
     2521 agcgctcgtc gacagcgagc tcaccgcggc cgagcgccgc gcacaaagac atgaactggg
     2581 tgtcgttgcc cacagccaac gcgaatcggc cgtctgacgc ggacacgatc tggtagggca
     2641 cgacggtggg gtgcgcgttg ccgaaacgct cggggatgtg gccggcggcc aggtaaccca
     2701 tgcccacgtt cgccagaaca ttgagcgcgc cgtcgagcag tgccaggtct atcagttgcc
     2761 cccggcccgt gcgctgccgc acgagcagcg cggccagcac ggcctgtccg gcattcatgc
     2821 cggtgaccag gtcggtgatg gccacgccca gcttcatggg ctcgccctct tcgtcgcccg
     2881 ttatggacat gagcccgctt tcagcctgga tgacgaaatc gtagcccggc tcggccgcgc
     2941 gcggcccggt gcgcccgtag cccgagatcg agcagtacac caggccggga ttcgtctgcg
     3001 acagggtctc gtagtccagc ccgaatttct tcagcgcgcc gagccggtag ttctcgacca
     3061 gcacgtcggc tttcccggcc agggaccgca ccagcgcctg cccctgcgga tgcctgagat
     3121 tgacggccgc ggaccgcttg ccgcgattgg tgcacagaaa ataagtggac tcgccgtcga
     3181 catccggcgg cgtccagatg cgcgtgtcgt caccgtgccc cggtgcctcg attttccaga
     3241 cgtccgcccc caggtcggcc agggtctggg tgcaccaggg cccggccagc acccggctca
     3301 ggtccagaac cttgatgcca tccagcggca ggggggtggg ggaactgctg tgttccacat
     3361 cttgcggctg attcatgcct gttccgcgga aagttggtca acaatgaacg ataacgcaga
     3421 ctggccgggt tggcgcaggc aggcttcgcc gatcacggtg gcccaatacg attccgagcc
     3481 ggcaatcagg cgccaggccc gcagccggcg cgtgtagagc tgcaggtcgt attcatcggt
     3541 gatgccgatg gcgccatgca ccgcatgggc gatgtcggcg atcagcgggg cggcctcgct
     3601 gcaacggctc ttgccgagcg cggccagcaa gggacgcggc tgccagcccg ggccgtggca
     3661 cgccatttgc gccgccatgc gcgccgccca ggcctgttcc gccatgacgc tgatctggtt
     3721 ctgcaccgcc tggaatcgac cgatggcttt gccgaactgg gtgcgttggc cggcatagtc
     3781 cagcgtcagg gacaacgcgc ggtcggcggc ccccgcgatc agggcggccc aggcggcggc
     3841 ctcgatgtca cgcaggcgca gcaggctgcc ggccggcagg gccggcacct cgcgggcgcc
     3901 atccggctgc gcgggccatc gcagggtcgc atccaggctg ccccggccac cggtcggttc
     3961 gatggtggcg cccgacaccg gcagcagcat ggtgccgccg tccagatcag ccagcaccca
     4021 ggcggccacc cgggcaaatc cgatgttccg ggcttccacc gcacccgcct tgcgggtggt
     4081 gccgacacct gccagggcga tggggccatc cggcgccggc acgcccgccg cagccagcca
     4141 ggcccgggcg agcagggttt gcacgaacgg caccggcacc gcgtggcgcc ccgccagcat
     4201 cagcaagggg aatgcgtccg acaggcccag gcccgcaccg cccgccgatt cggggaccag
     4261 ggcatccagg aatccggacg cctcgacggc ccgccatatc gtgtcggggc tgccgccctc
     4321 ttcgacatcg cgcaccgcca ccggcacggc gacttcggca aacaggcgtt caaccggttc
     4381 gacaaacggg ttttccatac atgtccttca gaatctgcca gattcaaatg tgcgccttgc
     4441 ttggcaagcc catgcatccg gataagtgta cgaaccaatc aaacagcaca ctagcgcagc
     4501 cccaggccgc gcgcgagaat accgcgcaag atctcgcggg tgccgccgcg cagcgagaac
     4561 gacggcgcca tctgttccag atacgccagg gtgcggccca gcgacgcggg catcggcaca
     4621 tcgggtgatg ccgccagaat gtcaccgacg acgcggggga tcatctgctc ggcctcggtg
     4681 cccagatcct tgacgagcgt ggcctccagc gcgggattct cgccccgggc cagcttcgcg
     4741 gtcacggcga tggacatcgc gcgcagcacg gccagcgacg ccaggatgcg tcccagtggc
     4801 gccgcgtggc gcaagccttc cgcgccaagc gcgcggcaat gcgtcagcca gccttcgagc
     4861 agcaccatgc tggaatacac ccgttcgggc ccgctgcgtt cgaaagccag ttcggcattg
     4921 acctgggccc agccctggcc ttcgtcgccg atgagcgcct ccggcgccag ggcgacgttg
     4981 tcgaaaaaga cttccgagaa atgcgcatcg cccgcgacat cgcggatggg ccgcaccgtc
     5041 acgccgggca ggctgaggtc gacaatgact tgcgacaggc ccttgtgccg gtcggcgggt
     5101 tcgccgctgg tgcgcaccag cgcgatcatg tagtgcgaac gctgcgcgtt agtggtccag
     5161 atcttgctgc cgttgagcac ccagccgtcg tcggtacgtg tggcgcgcgt gcggatgctg
     5221 gccagatcgg aaccagaccc gggttcgctc atgccgatgc agaagaacgc ctcggcccgg
     5281 cagatgcggg gcaggtagaa acgcttttgc gcttccgtgc cgtacttcag gatgagcgga
     5341 ccgctttgac ggtcggcgat ccagtgggcc gacaccggcg cgccggcgcc cagcagttcc
     5401 tcggccaaca cgaagcgcgc gaacggcccc ttgccggcgc cgccgtattc cacgggcagc
     5461 gtcaggccaa gccagccctt gtctgccagc gcgcggctga acgccgcgtc gaaccccatc
     5521 cacgaccggg cccggacctc gggtgcgagg ctccccaccg cttcctcaag gaaggcccgg
     5581 atggcgggcc gcagtgcctc gtcttgcggc ggaatgcccg ccagttcaag cgtatcgatc
     5641 atggttgcag tggccgcgcg ccgcgatccg gggggcaccc gcgccacgct gcaagcctgt
     5701 tcgctgttcg ttttccagcg gatactatcg gtgccgcaag cgcattctgt caaatattat
     5761 ttggcgcggt ttcgatactt gaacgctatc agtaaaggtc gcatccattg tgggctcggg
     5821 ccattaacag ggagccggcc aagcatggcg aactccgggc gccatgaagc cgaagcgaaa
     5881 ccgatagccg aaaccgatag ccgatagctg atagccgata gctgcagtgt atcgatgaag
     5941 ggaaaaataa tatttgaacc gcgcgtctcg acccattacc cttgactgtc agaacttcca
     6001 ccgcaaccag gccacaccat gacttcactg ctgaacttcg accaggacga ccaaggcgtc
     6061 gtcaccttga ccatgaacga cgacgccacc cgcaacgccc tgacgggcag cgccatggtg
     6121 gacgaattcc tggccgcctt cgaccgcatc caggccgacc accacgtcgg cgccgtcatc
     6181 atcacgggca acggcaaggc attctcgtcg ggtggcaata tcgacgaaat gggccggcag
     6241 acgcgaccgg aatacacccc cgcggcgctg cggcacgaat accgccaggg tatacagcgg
     6301 ctgccgctgg ccatgcaggc gctggaagtc cccaccatcg ccgccgtcaa cggccccgcc
     6361 atcggcgccg ggtgcgacct ggcctgcatg tgcgacatcc gcatcgcctc cgaacacgcc
     6421 ctgttcgcgg aaagtttcgt caagctgggc ctgatagcgg gcgatggcgg gtcctggttc
     6481 ctgccgcgcc tgatcggcat gtcgcgcgcg gccgaaatga cgttcacggg cgacgccgtc
     6541 gacgcccggc aggccctgga atggggcctg gtgtcgcgcg tggtcacggc cgacgcgctg
     6601 atggacgaag cgcgcggcct ggcgcaccgc atcaccgcca acccggccga cgccctgcgc
     6661 atgagcaagc gcctgctgcg cgaaagccaa atcggccgcc tggataccgt cctggaaatg
     6721 gcagccgcgt tccagtctct ggcgcacaag tcggacgacc atgcccgggc cgtgcgggac
     6781 ttcctggaac ggcgggcggc gcggaagaaa tagcggttat tgtccgggcc cgggtccttc
     6841 ccgcagaagc ggcgacatgg ggggcgccgc gtggactata gtgggaagtc aatccattcg
     6901 tattggagac tcctggcatg acaacgcaac aaccggcggc cgcggccggg caattcctca
     6961 ttggcggcga cctgtccatc aaccggctcg gcttcggcgc catgcgcatc accggccacg
     7021 gcatctgggg cgacccggcc gacccggccc aggcacgcgc cacgctggcg cgagcgcccg
     7081 agctgggcat caacttcatc gacaccgccg acagctacgg ccccaccatc agcgaagacc
     7141 tgatccgcga agtcctgcac ccctaccccg gcatggccat cgccaccaaa ggcgggctgg
     7201 tgcggcacgg ccccgacatc tggatgccgc tgggacgccc agaatacctg cgcctgtgcg
     7261 tactcatgag cctgcgccgc ctcggcgtcg aacgcatcga cctctggcag ctgcaccgca
     7321 tcgaccccaa ggtgccggag accgagcagt tcgaggtcat cgcggaaatg cacaaggaag
     7381 ggctgatacg acacgtgggg ctcagtgaag tgaacgtgac gcaaatcgaa gcggcgcaga
     7441 agcatttccc cgttgccacc gtgcagaacc gctacaacct cgtcgaccgc cagagcgaag
     7501 acgtgctgga ctactgcgag cggcacggca tcggcttcat cccatggtat ccactggccg
     7561 cgggcgcact gacccgtccc gactccgccg ccacgcgagt cgccgatcgg ctgaagatca
     7621 cacatgggca actggccctg gcctggctgt tgaagcgctc gccggtgatc ctgcccattc
     7681 ccggcaccag ccaggtcgcg cacctggaac agaacacggc ggcggccacc atcaccctgt
     7741 ccgacgaaga cttcgatgct gtcgatcgcc agggccggga agccggctag gcgccatcca
     7801 ctcccgaagc cgccctgctc cgaggcgggg cggcgtgaca ccgacacgcc aatgccatcc
     7861 gggacgccct caccccctaa aaaaccggcg caggatcccc gccctctggc gccgcgccgc
     7921 cccggcaacg acgagtgctg cggcaacggc tgcattccct gcatattcga tatctacgaa
     7981 gaccagctgg aacgctatcg ggaacagctg gcggcatggg agaagcggca acggcgcccg
     8041 ccccgcgcca caacttgccg cgcccacgcc ccagactcat aatccccaca ctctcaggag
     8101 cccacaccat accggaaaac gcgcatgatc gacttcaagc aaatcgaggc cttcgtttgg
     8161 gtggccgaac tgggcggctt ccgcgcggcc gccgaaaagc tcaacaccac gcagccagcc
     8221 atatcgcagc gcatagcggc catggaacat tcgatgcacg tacgattgtt cgagcgtggc
     8281 gcacgcggca tcaagctcac ggagaaaggc caggaattgc tctcgcatgc ccagcgcatc
     8341 ctggacatgc gatccgaaat gctgcgcgtg gcccaggaag aaaacactat tcgtggcatg
     8401 ctgcgcttgg gtgtcgccga aacactcgtg caaacctggc taccgcggct gatcgaagag
     8461 ctgcacgaac gctacccagc gctcatcgtc gagatccatg tcgacaccag ccacttcctt
     8521 cgatcacagc ttgcgtccta ccagatcgac ctggccctgc tggtcggcac gtcgcaagat
     8581 ccgaaagaag tccatatgca cctgtgtgac tacaaactgg cctggatagc cagccccgcc
     8641 ctgaagctcc acgaacacac cgccaccatc agcgagctcg gcgcttatcc cgttattacc
     8701 tatccctccc tcagcctccc ctaccacgcc gtgaaagacg ccctgaccca ggccggcatc
     8761 aagacgccca gaatctactg ttgtgcctct ttaagcacca tggtgcacct tacccgatac
     8821 ggtatcggac cgagcgtgat cgctcccgcc gcgatcaccg atgaactggc cagcggcaag
     8881 atccgcctgc tggacaccac cgttcccctg ccagacctgc gcttttatgc atgctggcgc
     8941 gattcacccg acagccacac ggcgcgcacc gttgccaggc tggcacgccg gatcgctctg
     9001 aaggcaaaac gcgaagaccg gcgccacgca taggccccca ccgcctgggc gaataaattt
     9061 ttcttatggc cccgtatccg aactaacaat tggacaagtc tggccaggta cggtgcaatc
     9121 gccaactgac aagaaccccc atcgcgattg ccccggactc ggatacccga ttcttcggtc
     9181 tccccgggac agcgaacccg tctttgccaa cctgcggaca tgcatgaccg cccccagagc
     9241 cggcaggccg ggccacatca cccgaaggag aagccccatg aaccgtttca tcttgtccac
     9301 ccttgccgcg ggcgccctgt tcgtcgcagg cagcgcctcg gccctggaca aagtccccaa
     9361 tctgccggaa acccacctgc aggtggtggg aggtctcagc aacctgaccg cctaccgcaa
     9421 tttcgaagtg cctttctgga ccaagaccgt gcccgagctg tccgacgggc gcgtcacagc
     9481 caccatcaag ggcttcaatg aaatgggcct gaaagggcct gaaatcatgc gcctgatcag
     9541 ccagggcgtg attcccatcg gcacggcgac gctggcctac tttgcaagtg acaacgccat
     9601 caacgaggcc atagaccttg ccgggctgtc cccgtcggcc aagatcgcgc gggaagtgac
     9661 gaacgccttc acacccgcct acgacgcata ctacaaaaaa cagggcgtca aagtcctggg
     9721 catctcgacc tatccagccc aggtcctgtt ttgcaatgca aaaatctcca gcctggccga
     9781 cctgaagggc aaaaaagtcc gcaccagcag ccgcaccacc gccgagttcg tccaggcgtt
     9841 gggcggcacc agcgtcacca tggcgttcgg ggatgtagtc cccgcgcttc agaacggcgt
     9901 ggtcgactgc ggcatcaccg ggtccatgtc gggctattcc gccaagtggt atgaagtcac
     9961 caaatacctg tacgcgctgc ccatcaactg gaaccagcag atccacgccg cgaacctggc
    10021 ctactgggac aagctcgatc ccaaggtgca ggcgttcctg cagaaaggca tcgatcacat
    10081 ggtggaccag atctgggatg ctgcggcgaa ggaaacccag gaaggctacg actgcaacac
    10141 cggttccgac aaatgcccgc tgccggtaaa gggcaagatg aagctggtcg aacccaccgc
    10201 tgcggaccgc gagttgctga agaagatcat gaccgatgtc gtcgttccca agtgggcggc
    10261 ccgttgctcc gcgcaatgcg tcaaggactt caacgacacc gtcggcaaac tgcgcggcat
    10321 caccgccaag aagtaatcgg agcctcccga ctccggtgcc cggcgggccc gcgcttctcg
    10381 aggcggcccg cctttccccc tatcgacgcc atcctatgaa caaccaagac cgccaagcgg
    10441 gcatcggcag actgctgacg ctcgcgcgac agctttcacg cgtggccatg tgggccgccg
    10501 gcagcctgac gctgctcagc gcgctgtaca tttccgtcga cgtcatctgc cggaaattct
    10561 tcaatatttc ccttggaggc tcggacgagc tgtccggcta cgcattcgcc atcagcattt
    10621 cctgggcatt gtcgttcgca acgctcgagc gggccaacgt ccggatagac gccctctacc
    10681 agacgctgcc agcgcgcttg tgcgcgctgc tggactggat ctcattggtg tgcctggctg
    10741 tcttcatcgt ctggctgaca cgctacgccg tcgatgtggc agccacctcc tgggtccggc
    10801 actcgaccgc caacaccgta ttggccaccc cattgtggat cccacaatac ctctgggctg
    10861 ccgggctgat ctggctatgc gtcgtcctgg ccctgatgct gctgcgttcc tcgattgccc
    10921 tggtcaccgg cgacctcgcc accatccggg ccatctgcgg catacgcacg actgcggaag
    10981 aggcttcgga agaagcggcc gccggcgtgc gcatggtgcg aggagacgcc gcatgatcac
    11041 caccgcgctc gtcctcctgc tggtcctgct cggactgagc attcccgtcg gcgcagcact
    11101 cggcgcgctg gggctcattc tcgatccgtt gtattcgatg ctgcctcttt cccgggccat
    11161 tggcgaaatt tcctggagca ccagcaacga gttcctgctg gtggccatcc cgcttttcat
    11221 catgctgggg gaaatcctgc tgcgctccgg actggccgag aaaatgtaca actcgatgag
    11281 cctgtggctg tcctggctgc cgggcggcct gatgcacgcc aacatcggcg ccagcgccct
    11341 gttcgccgcc acgtcgggct ccagcgtcgc cacggcggcc acggtgggta ccgtggccat
    11401 cccacagatc gccaagaagg gctacaacga atcgcttttc ctgggcagct tggccgcggg
    11461 aggcacgctc ggcatcctga ttccgccgtc cataaacctg gtcatctatg gcgtgctgac
    11521 caacacctcc gtgccgaagc tgtatctggc cggcatcatc cccggcatcg gcatggccgc
    11581 gctgttcatg ctggcgatcg gcgccgcatg cgtgctccga ccgaaatggg gcggccagaa
    11641 aatctcggcc acgtggggac aacgcatggc gagcctgaaa cacctggttc ccccgctgtt
    11701 catcttcctg ctcgtcgtag gctcgatcta cgccgggctg gccacgccca cggaagccgc
    11761 ggcgctgggg gtcgtgggcg cgctgatcct tgccgcctgg gaaagaaagc tctctttcac
    11821 catgctgcgg gatgtgctgg aaggcactat gaaagccacg gccatgatca tgctgatcgt
    11881 cctcggggcg gccttcctga acttcatcat gtcgggcacg gggttgacca cggcggtgac
    11941 caacacagtg gcgcatcttg gcctgtcgaa aagctggatg ctgctcatca tggtggcgtt
    12001 ctacctggtg ctgggctgct tcatggaaac cctgtccatg atgatcacga cgattcccat
    12061 cgtcgcaccg atcatgatcg cgctgggctt cgacccggta tggctgggcg tcgtcatcat
    12121 cattctcgtg gaggccgccc tgatcacccc gccagtgggc ctgaacctgt tcgtcatcca
    12181 gagcctgcgc acacgcggca gcatgaacga cgtgctggcc ggcagtctgc ctttcgtcat
    12241 catgttattc gtcatgatag gcctgctcgc cctgttcccg aatctcgcac tgtggctacc
    12301 gcacgccttc ggttgacatg gcgcatttca cttcttcctt actcatttac atcctgatgc
    12361 cgcatcatga agctatcgtt tgaactctcc tctgcaaccc gccccgaaac cgtggaagtg
    12421 gatttccagc aatgcattgt cgccggctgg gctggccggg atcttgccgc cacggaacat
    12481 cacattgaag aactcgctct gctgggcgtc acacgcccca gttccgtgcc attgttctac
    12541 cggatcgcat ccaatcaatt ggtacaggac ccgatcatcg acgtcgtggg tgaagaaacg
    12601 tcgggagagg ccgaggtatt ccttttctca catcagggag aaacgctggt cagcctttcg
    12661 tcggatcaca ctgatcgcaa gctggaggct cacagcgttg cactctccaa acagatctgc
    12721 gccaagccgc tggcccgcca tgcctggcgc catgccgagg tcgcagatca ctgggacgac
    12781 ttgattctcc ggtcgtggat caaggaagac ggcaatcccg tgctctatca ggaaggcaac
    12841 ctcgggacgc tccttccgcc actggaactc gcacagcgtt tttttggctc gatccgtgtt
    12901 cccgaaggct ccggcatgac ctgcggcacc gtctcgacaa ttggcgcaat acggcccgca
    12961 tcaggtttga aaatggaact gttcgaccct aagctcggtc gcagcatcac gcacgcctac
    13021 acactctgcg tcctacccga aatagcctga tcctattggc acagagcggg gaatatactc
    13081 gtccaccatg tgtggccgga tctccccgca ctcttccatc ccgtcgtcca aaggaattct
    13141 ccatgctccc cacccttctc tccctgcaga atgatctcgc cgaaggccgc accacctcgg
    13201 tcgcactcac cgaagccgcg ctggcacgca tcgatgacac gcagggcgaa ggcacgcgcg
    13261 cttttaccaa ggtctatcgc gaacaggctc tggcggcggc gcaggcgtcg gatctgctgc
    13321 gggcggcggg cctggtgcgc tcgccggtgg acgggctgcc gatttccatc aaggacttgt
    13381 tcgacgtggc gggcgacacc acgccggccg gctccaaggt gcttgaaggc gccgaaccgg
    13441 cgcacgagaa cgccgtcatc gtccagcgcc tgctgagcgc gggggccgtc atcgtcggtc
    13501 gcaccaacat gagcgaattc gcgttctcgg ggctgggcct gaatccacat tacggcaccc
    13561 ccagcagccc ctgggaccgg gccacaggcc gcattcccgg cgggtcgtcc tcgggggccg
    13621 gcgtttccgt ggccgacggc atggcggcgg ccgccattgg caccgacacg ggggggtcgg
    13681 tgcgcattcc ctcggccttc tgcgggctga cggggttcaa gcccaccgcc gcgcgcgtgc
    13741 ccacgcaggg cgcgctgccg ctgtcatgga cgctggactc catcggcccg ctggcggcct
    13801 cggtggcatg ctgcgcgatc ctggacgccc tgctttccgg caccgtctac cgcgcaccgg
    13861 tcgcgccgcc actggaccat ctacgcctgg ccagcatctc ggccatctcc ctcgcggggg
    13921 cggacgagca tgtgacacaa acctatcggt cgacactcga cgcgctggag agccagggtg
    13981 ccgtgatcga ggaaatcagc ctgcccgaat tcggcgaact gccgcacatc aatcacctgg
    14041 gcggcttcgc gggggcggag tcctgggcct ggcatcgcca gttgctggcc gagcacgccg
    14101 ccgagtacga cccacgcgtg tccgcccgca ttgcgcgcgg ccaggccatg aacgccgccg
    14161 attacatcga cttgcaacgc ctgcggccgc aatggatcac cgccgtggag acgcgcctgt
    14221 gcggctacga cgcgttgctc atgcccacgg tgccggtgac cgcgccggcc atcgccgacg
    14281 tggcagcctc cgacgacgcc tatttcgccg ccaacgggct catcctgcgc aactcgacgt
    14341 atatcaattt cctgaatggc tgcgcgttgt cgctaccctg ccacgccgct ggcaccgcac
    14401 cagtcggcct gatggtggcg gggtgcgcga acacggacgc ccacatcctg ggcgttggcc
    14461 aggcgctgga aggggtgctg gcgcgaatcc gcggttgatc acaaaatcgg gcgatgctct
    14521 agaaaccgct caggccttct ctttcaggaa tgccaccagc gatttgcccg cattgacgat
    14581 gtgatcgcgc gtgaggtgcg ccgccgcctt cgcgtcacgc cgcaggcagg catccatgat
    14641 ggcctggtgc tcgacgcggg cacgctgctt gccgtgggtc agatcaagct gcgcgcgaat
    14701 gtagcgctcg acccgatcgc tggtggcgtt gacgatggac aggtaataag gacggttcgc
    14761 gtcggcatac aaggcagcgt ggaattgtcg attgatgtcg ccccagcgct cgggccgggt
    14821 ttcggcggag aacgcatcac aacacctttg ggcctcttca agggattcgg ccgacaattt
    14881 cggcacggcc agttcgatga cgcgcggttc gaccagcgcc cggaattcga aaatttccga
    14941 aatctcgtcg acggacatgg atgccacgac cacgcccttg tagcgcacgg acgtggccag
    15001 cccctgggat tccagctttt tcagggcctc gcgcacggga atgcggctga cattgaacat
    15061 ctgggcgatg ttgtcctggc gcagcgtttc cccctcgggc aattgaccac gaatgatggc
    15121 atcgcgcaac gcgccataga tcacatccgc ggccgacgcc gtcttgccga gatcgaccgc
    15181 gcttattcct gaccagtcgt ccacatcaca cccctagtgt cctgtttgac tcattcggct
    15241 gcttatctcc ggcgcctgga ctcgccatca tgcggccacg ttgtcctggt cgcccgaaca
    15301 gacggcgcct cgccccgtgt gccgccggat cacgcctatg cgtcgaatca cgccaatgtt
    15361 atctgattcc ctttcaatcg agataaaaga tattgaatat tggatccaat tatgccatga
    15421 tagcggaaat ccctcggcgc atccatctcg gcctccgccg gcaacgcgcg ccctcaccca
    15481 ggagaaaccc catgtggact ggtgtccttc ccgcagtaac gactaaattc acccaagacg
    15541 gcgcgctcga ccacgctgaa atggaacgct gcttcggcct gctcatggaa gcgggctgcg
    15601 acggcctgat cgtggcgggt tccctgggcg aaggccccat gctcacccac caggaacgcc
    15661 tcgacgtcct gaaaaccgcc caaaacgtcg ccggcaaacg ccctgttctc atgaccgtcg
    15721 ccgcagcctc cacccaagaa tcctgcgacc tggccggcaa ggccgccaag gccggcgcct
    15781 cggggctcat ggtggtgccc agcgccatct accacacgaa tgaagtggaa accgaagcca
    15841 cgctgcgcgc cgtcgccgcc gcgggcgatc tgccggtcat gatctattcc aaccgcattg
    15901 cgtaccgtgt cgacgtgaca ccggaaatca tggaacgcct ggcggacgac aaacacttcg
    15961 tcgccgtcaa ggaatcatcc gacgacatcc gccgcaccat tgaaatattc aaccgcgtgg
    16021 gcgatcgcta caacgtgctc accggcgtcg acaatctggc gttcgaagcg ctgtcggtgg
    16081 gcgccgtggg ctgggtggcc ggcctggtcg tggccttccc gaaggaaacc gtcgccatct
    16141 accgactcat gaaggccggc cgccgcgacg aagccatggc catctaccgc tggttccgcc
    16201 cgctgctcga cctggacgtc agcacctacc tggtgcagaa catcaagctg gccgaggcgc
    16261 tggccatcga ttcgaatgat cgggtgcgcc tgccgcgcat gccgctgcag ggcgaacgcc
    16321 gcgcccaggt gcaggccatc gtcgagaagg ccctggccac gcgcccgcag ttgcccgact
    16381 acctgaaact cgccgcctga cctccgcatc ggccgcgcac ccgggttggg gcacggccgg
    16441 cccccaaccc ggtatcacgc aggaagccag ccatgcaatc cagcaaggtc atccacgtcg
    16501 tcagctgcca cgctgagggg gaagtcggcg acgtcatcgt gggtggcgtg gcgccgccgc
    16561 ccggcgccac gctctgggaa caatcgcgct ggctccacca cgacaagtcc cttcgcgact
    16621 ttgtcctgaa cgagccacgc ggcggggtgt tccgtcacgt caacctgctg gtgcccccaa
    16681 agaatccgcg cgccgtcatg gggtacctga tcatggaacc ggtgcacacc ccacccatgt
    16741 ccggctccaa ctcgctgtgt gtggccaccg tgctgctcga caccggcatc gtacccatga
    16801 ccgaacccga aacccgcttc acgatggaag cgccgggcgg gctggtcgat gtggtggcgc
    16861 aatgccgtca cggcaaagcg gtatccgtga aagtcgtcaa tcacccgtcc tttgccgacc
    16921 ggctggacgc ggtcatcgaa gtggcggggc tggggtcgct gcgagtcgac accgcctacg
    16981 gcggcgacag cttcgtcctg gccgacgcgg ccgcgctggg ctttgcactc acgcccgacg
    17041 aagccgccga cctggccgcc accggtatgc gcatcacgcg cgccgccaac gagcaattgg
    17101 ggttcatcca ccccaccaac cccgattggc gccatatttc attctgccag ctcaccctgc
    17161 cggtcacgat cgtcgacggg atcgccacgg gccgcaacac ggtcgtcatc gagcccggca
    17221 aactcgatcg ctcccccaca ggcactggct gttccgcgcg catggccatc ctgcatgcgc
    17281 gcggccaact gaaagaaggc gacgccttca ccggcctgtc cattctcgat tcgcgcttcg
    17341 actgccgcat cgaaggcctg acctccctga gcgggaaggc cgccatcatc cccagcattg
    17401 cggggcacgc ctggatcacc ggcacgcacc agcacctgct ggatccgtcc gacccgtggc
    17461 cccggggcta ccggctgtcc gatacctggc cgaaccccga ccagcgctga gcaaagcccc
    17521 cgggtacagg aacccccgca cactcaatca ctgcgcgccg tccgggcgcg cccacaaacc
    17581 tcgcgttaac cctaggtttc aaggaaatat gggatatacc ccgacggaaa atcattgacg
    17641 cttcaagtgg cgcttggcta agatcgtcgg ctagtgttct gttcggctgg ccggcatgcc
    17701 caatccacgc gcctgatccg gccaggcgga acgccggggt gaacagccct gaacaacgga
    17761 gcgtctttat cttgaagcag cagtctctcg acatgatcac ccggctcgtc gggttcgaca
    17821 ctgtctcacg caattcgaat ctcgcactca tcgactacgt gcgcgattac ctggccggcc
    17881 acggtgtgca aagccacctg gtccacagcc caaaaggcga caaagccaat ctgttcgcca
    17941 ccatcggccc caacgtcgaa ggcggcgtgg tgctctccgg gcacaccgac gtggttcccg
    18001 tcgacggcca gccctgggac accgaccctt tcacggtcac cgagaaagac ggccggctct
    18061 atggccgcgg caccagcgac atgaagtcgt atctggccat cgccctggcc atggtgcccg
    18121 acatgctttc gcgcgacctc aagcggcccg tgcatctggc cctcagctac gacgaggaaa
    18181 tcggctgcgt cggcgccccc tccatgatcg accgcatggc cgccgatctg ccccggccgg
    18241 gcgcggtcat cgtcggcgag cccaccagca tgcgccccat cgtggcccac aagggggtcg
    18301 ccgcgctgca caccaccgtc atcggccacg aagcccattc cagccaggtg caacgcggcg
    18361 tcagcgcggt catgatcgcg gcgcgcctca tcaccttcat cgacgacatg atggccgaga
    18421 acaaggccgc cgccgatccg caatgcccgt tcgtcccgcc ctacaccacg ttgcacgtcg
    18481 gcgtcgtgca cggcggcacc gcgctgaaca tcatttcgcg cgagtgctcg ttcacatggg
    18541 acatccgcgc catgccgggc gacgattggc acaagtacct ggaacgcttc aacgcgtatg
    18601 cggaaagcct gctgccggcc atgcgcgccg tatcgcccga cgtgtccatc accacccagg
    18661 ccttcgccga ctcccctccg ctgcaggatc agggcggcgc cgcccaggaa ctcgtgttca
    18721 aactgaccgg ctgcacttgc ggcgacgtcg tgccctacgg cgccgaaggc ggtcagttcc
    18781 aggaacgggg gttcccggtg gtgttgtgcg ggccgggctc catagaccag gcccacaagc
    18841 ccaacgaata catcgacatt tcgcagatca gcgcctgcga ggctttcttc ggccagctgc
    18901 tcgaccggct cgctcactag gtgggtgggc gcggcggttg ccgggcccca tcgcgcgaat
    18961 tgctcgcgcg gtggggcgaa ttccgacgcc gggcgcccga ttcgccaagt ggcgtgccaa
    19021 tctccgggaa aaacacaata cgacgcgaca tccacgcagg atatatttga aacccgcggc
    19081 cccgcccggc ttcaatatgc ggtaccaccg gcccgcgtaa cgggtcggcc aggcccaagg
    19141 cggcatcggg gccatccaaa aaaactcaac ggcaggagac tcagtatgca gatatcccga
    19201 ttcatcaaac acacggcgct ggccagtgcg ctcgcgctcg gcctggggct gggccacgcc
    19261 gccacggcca agacgttccg ctggtcctac cagggcgacg tcaactcgct cgaccccatg
    19321 tcgctgaacg agaccttcac cctcggtttc cagaactcgt tctacgaacc gctggtcaca
    19381 tacgacaaag acctcaagct ggtgccggcc ctggctgtca aatgggaaaa cccctcgccc
    19441 accaaatgga ttttccacct gcgcaaaggc gtgaaattcc acgatggcga gcctttcacg
    19501 gccgacgacg tggtgttctc ctggaagcga agcctcacgc ccggctccga catgaaaggc
    19561 tacggcagcc tggccaccga cgtgaagaag ctcgacgact acaccgtcga agtcaccacg
    19621 cccacgccca atcccattct gccgcgcgac tgggtgttcc tgtacatgat gagcaagtca
    19681 tgggctgaaa agcaccacac caccgacgcc accaacgtca agggcggcgg cgacaactac
    19741 gccaacatgc acgaagacgg cacaggcccg ttcgaggtca ccagccgcca gcccgacgtg
    19801 aagaccacgc tcaagcgctt ccccggctac tggaacaaga acatcccgac caacgtcacc
    19861 gacatcgtgt tccagcccat caagcaggaa tccacccgcg tcgcggcgct gatatccggc
    19921 gacatggacc tggtgcgccc catccccgtg caggattggc cgcgccttga aaaagtaccc
    19981 aacatcgaac tgctgcgcaa acccgaggca cgcgcgatct tcatcggttt cgaccagcac
    20041 cgcaaagaat tgctgtactc caacgtgaag ggcaagaacc cgttcaagga caagcgcgtg
    20101 cgcgaggcca tcgacctcac cgtcgacacc aacctcatca acaagaagat catgcgcggc
    20161 gccgcgcatc cgttgggcac gctcatcgcc acgtccatca acggctacga tccttccttc
    20221 ggcaagccct acaaggtcga catccccaag gccaagaaac tgatggccga ggcggggtac
    20281 ccgaacggct tcgaagtcac gctcgactgc ccgaacgacc gctacgtcaa cgacgagaaa
    20341 atctgccagg ccgtggccag catgctggcg cgcatcggca tcaagatcga cctcctggcg
    20401 cagaccaagt ccaagtactt cggcaaggtg ctgctgcaag ccggcaacaa gaccagcatg
    20461 tatctgctgg gctggacgcc aagctcgatc gacgccgaga actccctgca aaacctggtc
    20521 gcctgccgca acgccaagac gtccgccggc cagttcaacc tgggtgggta ctgcaacccc
    20581 aagatcgacg acctgatcgc caagatcggc gtcgagaccg accaggccaa gcgcgatgcc
    20641 atgatcaagc aggccttcat gttgctgcgt aacgattacg gctacctgcc cctgcaccag
    20701 caaccattgt cgtggggcgt gcgcaagggt gtcaaagtcg agcagcgcgc cgacgacgtt
    20761 ctcgacatgc gctacgtcgt catgccgtaa ggcatttcct gccgaccagg cgtagcgccc
    20821 ggcccggggg cacattcccg ggccgggcca caccacggcg cgtcgcgccg gaagatttcc
    20881 accatgctga acttcatcat tcgacgcatc atccagtcga tcttcgtcat gctggccgtc
    20941 gcgttcatcg cgttctcgat gttccgctac gtcggcgacc ccatcaacaa catggtgggc
    21001 caggacacca ccatggccca gcgcgcgcaa ctcaagaagg cgctggggct ggacgacccg
    21061 ttcatcgtcc agtacgcccg cttcatccgc aacgccgcgc atggcgactt cggtgtgtcg
    21121 taccggcagc gcgtgccggt gcgcgcgctc atcgccagcc gcatgcccgc cacgctcgag
    21181 ctgtcattcg cgtcagccat catcgcgttg ttcctgggca ttcccatggg catctatacg
    21241 gccctgaagc ggcgcggcgt gctgtcgcag tcgttcatga cgatctcgct catcggcatt
    21301 tcactgccta cattcctgac cgggatattg atgatcctgg tcttcggcgt cctgctgcgc
    21361 tggctgccca cgttcgggcg cggcgaagtc gtccacatag gctggtggag ctcgggcttc
    21421 ttcaccaaga gcggctggga atcgatcatc atgccggcct ttaccctggc gttgttccag
    21481 atgacactca tcatgcgtct ggtgcgcgcc gaaatgctgg aagtgctgca gaccgatttc
    21541 atcaagttcg cgcgcgcccg cggcctaccc gagcgcctga tcaacttccg ccatgccttg
    21601 aagaacacgc tggtgccggt catcaccatc accggcctgc aactgggttc catcatcgcg
    21661 ttctccatca tcaccgagac cgtgttccag tggcccggca tggggttgtt gttcatccag
    21721 tccatcgagt tcgtcgacat ccccgtgatg gcggcgtacc tgctcatgat cggcttcttc
    21781 ttcgtgatca tcaatctggt ggtcgatctc ctgtacttcg ccgtcgatcc ccgcctgcgc
    21841 gtgcagaagg gttaatggcc cgtccagact aatcgatccc aagacacgac atgactacca
    21901 ccacgctttc ccgcatccgc aacagcgacc tgtactacag cttcacgcat tcacccgcgg
    21961 cgatgatctc ggcgttgatc gcgctgctca tcttcctggg cgccgtgttc gcccccctgg
    22021 tcgcgccgca caaccccttc gacctggcgt cggtgaacct gatgaacgcc aacatgccgc
    22081 cggcctggtc gcctgacggc agccacgcgt tcctgctggg caccgacgac cagggccggg
    22141 gcatcctgtc caccatcctg tacgggtcgc gcctgtcgct ggaggtgggc ttcgcctccg
    22201 tgttcttctc gctgatcatc ggcgtgacgc tcggcctcat cagcggctac gtcggcgggc
    22261 gcatcgacgg cttcatcatg cgcatcgccg acgtgcaatt gtcctttccg gccatcctga
    22321 tcgcgctgct ggtcgacggc gtggcgcgcg gcctgctgcc ccgcgacatg caggacaaaa
    22381 tggccatcta cgtgctgatc tttgccatcg gcatctcagg ctgggtgcag tacgcgcgca
    22441 cggtgcgcgg cgcaaccatg gtcgaacgca acaaggaata cgtgcaggcg gcacgcttga
    22501 tcggcatgcg cccgttcacg gttctgcggc gccacatcct gcccaatgtg ctcggccccg
    22561 ttctggtcat cgccaccatc cacctggccg tggccatcat caccgaagcc acgctgtcgt
    22621 tcctgggggt gggcatatcg cccaccacgc cgtccctggg cacgctcata cgcatcggct
    22681 ccaactacct gttctcgggg gaatggtgga tcaccatctt ccccggcgcc gccctggttc
    22741 tgctcgccct gtcggtgaac ctgctgggcg actggctgcg cgacgcgctc aaccccaagc
    22801 tccgctgact agggcctgtt caccatggca ttgctcgaca tagaaaacct gaagatcgaa
    22861 ttccccagcc gacgcggcga tctggtcgcc atcgacgggg tgtcgctcgc actcgaaaaa
    22921 ggcgagatcc tgggcgtggt gggggaatcc ggcgccggca agtcgaccat cggcaacgcg
    22981 gtcatcgggc tgctgcaggc gcccggccgc atgacgggtg gcacgatcag cctgcaaggc
    23041 aagcggctcg acgaactcaa gccgcacgaa ctgcgcctgg tgcgcggccg gcgcatcggc
    23101 atgatcttcc aggatccgct cacttcgctc gacccgctgc agaccatcga gcgccagctc
    23161 gtcgaaacca tgcaggtgca cctgcatctt tcagcggccg aggcgcgccg gcgcgccatc
    23221 gacatcctgg acagcgtcgg catcgaagac gccgccgtgc gactgcagca atacccccac
    23281 cagttctcgg gcggcatgcg ccagcgcgtg gtcatcgcgc tggccttgtg ctgcgagccg
    23341 gaagtcatca tcgccgacga acccaccacc gcactcgacg tctccatcca ggcccagatt
    23401 ctggaactcc tgcgcaagct gtgcaaggaa aagcaggttg gcatgatcat catcacccac
    23461 gatatggggg tgatcgccga aatcaccgac cgcgtggccg tgctgtaccg gggcaaactg
    23521 gtcgaggaag gcaccaccgc caagatcctc ggcagccccg accaccctta caccaaaagc
    23581 cttatttccg ccgtgccgcg atccgacgtg aaactacgcc ggttccccat ggtgacctac
    23641 atcgaggacg ttcttgcccc acgaaagagg gaattggata tctccacgca ttggcttggg
    23701 caggaacgcg attttggcaa acacgaagac acggcgctgc tcgacgttca agacctgagc
    23761 atgcgcttca tcctgcgcaa tgcgttcctg aagaagaacc gcctcacttt cgacgcggtc
    23821 aagcacgtca atttttcgat cagggaaggc gaggtcttcg gactggtcgg cgaatccggc
    23881 tcaggcaagt cgaccatcgc gcgcctgatc gcggggctct acaaggccaa caacggctcg
    23941 atcatcttcg acggcacgaa cgtctcgtcc ctcaaggacg aacggcaact gaacgccttc
    24001 cgccgccaga tccagatggt cttccaggat ccgttctcgt cgctcaaccc gcgcatgcgc
    24061 atcctcgaca tcgtggccga gcccatccgt ttccacaggc tcacgaccag cgaggcgcag
    24121 acccgcgaga tcgtcagtga tctgctcgac tatgtcggcc tgggcgacca ggccctgcgg
    24181 cgcttcccgc atgagttctc aggcggccag cgtcagcgca tctgtatcgc gcgcgccctg
    24241 gccacgcgcc cccgcttcct catctgcgac gaacccacct cggcgctaga cgtgtccatc
    24301 caggcacaga tcctcaacct gctcaaggac ctgcaagaga agctgcatct gaccatcctg
    24361 ttcatcagtc atgacctgcc cgtgatacgg cagatgtgcg accgggtcgg cgtcctgcgc
    24421 cacggcgaat tgctcgaagt ggccgagacc gaagcgctgt tctcgaatcc caggcatgaa
    24481 tacagccagc acctgctgtc gctcatgccc acaatgcatt tcatgtcgcg cgaggggctg
    24541 gagctcgagg cgtcggccgc ctgaccccca gggccccgcc gaagatcttt tcactttttt
    24601 acacgatatt gttgttctat tgtcgcgccg tgtctggacg gccatgggcg ctccccctat
    24661 gattgagccg ttgtcaacgc gtgcccggtc cggtgcgcaa catcttccac ttgtatatac
    24721 atacacaaag gaaaaaccat gcacatcatc ggcatgatca tcgttggttt catcatcggc
    24781 ctgatcgctc gcgccatcat gccgggtgac cagaagatgg gcctcatcat gaccacgatc
    24841 ctcggtatcg tgggctcgct ggtcgccggt tacatcgggc aggcgctgca ctggtatgcg
    24901 ccggggcaag gggctggctg gatcggctcc atcatcggcg ccatcatcct gctgttcatc
    24961 tacggcatgg tcaagaagaa ccagtaaacc gccacgccgg cgacaatcca ccttgcaaga
    25021 cgcccttcgc gggcgtcttg tctttggtgc ggggccttcc gcggcgcacc gtcaatcgat
    25081 cgtgccgccg cctcaaaacg acccgataca ataaggccga acagaccgct tcccgccacc
    25141 aatcttcccg gaggccacca tcgagccatc ccgccattcc aatcgccccc gggccgccgg
    25201 ggccacccat tcgtgaagcc cgacggcgcg cacttcaccg attactggct ggctgaatgg
    25261 ctgcgcctgc gcgaagcgca ctgggggccg ctggaagacg cgcgcgaagt gcggcaggcc
    25321 cgcgcccagg ggcggacatt cgcccagcgc atcgtgctgc gcgcgcgctt cctatgcgaa
    25381 cgcgagaaac tcgacaccgt gccggcgcgc tggtggcaag gcgcccgtct gattctgccc
    25441 gcgctgggcc tgctggcgat cctggccgga tcgggcgcgg cgctgggcgc cctgggaaac
    25501 ggcatcgttc ccgtcaacat cgccctggcg ctcaccgcca tgctggggct gcactgcctg
    25561 acctaccttc tctggctcgc cagcttcgcg ctgcacggcc atgccgaagg cggcgcgcag
    25621 ctgggtcgtc tttggttgtg gttgacccgc aaactcgcgc gcggccccga tgccgccttg
    25681 gtgcctcgcg cgctgacggg gctactcaac cgcaacggct ctctgcgctg gttgctgggc
    25741 agcgtcagcc acggcctatg gactgtggcc ttcaccagtc tgttgctcac gatgctggcc
    25801 gtgttgtcgg cgcggcgcta cgacttcggc tggcagacca ccctgctcac gcccgatacg
    25861 ttcgtgcacc tggccgccct tctgggctgg gcgcccgcgc atctgggctt cgccaccccg
    25921 cccgcggaca tcgtgcgcgc gagcaatggg ctgcacacgc tgcccgatac ggcgcgcgcg
    25981 ctgtggtcga actggctcat gggctgtgtg gtggtctacg ggctcctgcc gcgtctgctt
    26041 ggcctggtgc tgtgcctggc catgacacgg taccgcatcc gccgcctgga cctcgacgtc
    26101 accctgccgg gttatgccga cctgcgccag cgcctagtcc catcgagcga ggtcaccggc
    26161 atcgacaagc cggacaccga catcgccacc gggcctggct tcgccacgca tccctcatcc
    26221 cccatcgccg gccgcccagc catcgccggc atcgaactgc cacacgacca gccctggccc
    26281 cccgtctccc tcgacgcgcg catcgtcgat ctaggggtgg tggattcccg cccccagcgc
    26341 caggccacgc tggagcggct gcaacactcc ccaccctccc ggctgctcgt cgtctgcgat
    26401 gcccgccaga cacccgaccg gggcgctgtg gcgctcatcg ccgagctcgc cgccgatgcc
    26461 ggcgccacga tggtggcatt gacacttccc gcgaatgaca cgccaacccc gcagcaccgt
    26521 gccggccagt ggcgcgaccg gctcgtcgat gccggcctgg cgtccgacct tatcgtcgaa
    26581 gacacccatc aagccctgtc ctggctgacc acaccgcccg gggcctcgaa tgagtgacac
    26641 cctcccgctc accctggccg tggtgggaca caccaacacg ggcaagacct cgctgttgcg
    26701 caccctcacg cgcaatcccg atttcggcag ggtcgacgac caacccggca ccacccgtca
    26761 tgtcgaaggc acacgtctgc tgatcgacga tcagcccgtg gtcgagctct tcgacacgcc
    26821 tggcctggaa gacagcatgg cgctgctgga ttatctggac cagatcgccg agcgcgcgca
    26881 gcggctggat ggtccagacc gcatccggcg ctttctggat tcgcccgaga gcaagcggcg
    26941 cttcgagcaa gaggcgcgtg tgctgaacaa actgctggcc tgcgacgcgg gtttgtacgt
    27001 cgtggacgtg cgcgacccgg tcctcagcaa gcacaaggat gaactgacac tgctcgccag
    27061 ttgcggccga ccgctgctgc ctgtgttgaa tttcatccac agccccggcg aacacagcca
    27121 ggtctggcgc gacaccctgg cccgcctggg cctgcacgcc agcgtcgagt tcgacaccat
    27181 cgcgcccgca ctggatggcg aagaccagtt gtatggcaag ctggcattgt tgatggacgc
    27241 ccacgccgcg ttgctgtccg aattgcgcga cgatgtgtcg cggcagcggc tgatacgccg
    27301 cgcggatgcc gcgcgcctga tcgccgagct gctggtcgat gcggcggcct ggcacgtcgg
    27361 ttgcgcgccc gacgacgaat ccgtgcgcca ggccaccgac accttgcgcc ggaagatccg
    27421 gcgacgcgag gcggattgcg tcaaggcgct gctcaagcgt tataacttcc ggaccgccga
    27481 cttcccacag cacgcgttgc cgctgcaggg agaacgctgg ggcatggacc tgttccaccc
    27541 gcaagccctg aaggacgccg gcctgcacgt cggcaagggt atcgcggctg gcgccatggc
    27601 cggcgtgacg gtggacgtct tcaccgctgg cctgagcatg ggcgcggcag cgctcgtcgg
    27661 cgccgcgctg ggcggcctgt ggcagggcac ggaccgcctg ggaaaacgca tcatgggccg
    27721 gctgaaaggc tttcgtgagc tgagtgtgga cgacgccgtg ctgcggttgt tgcaagtgcg
    27781 ccagttggcg ctggttcggg ccctggagcg ccgcggccac gccgcgcagt cccccatcgt
    27841 gttggcagcg gacggtgcgc ccccaaccat ggattcgtca ccggaggaaa cccgcaccgt
    27901 ggcggtggcg cggcccgccc cgcccccgtt gcccgatgag ctgaccgagg cacgcagcca
    27961 accccagtgg tcgacgctgg acaagcacta tgcgcacagc ccccggcgcg accagatcgt
    28021 gcgggcgctg gcggaaagac tggcaccctg acgggcacaa cacaatgatg ccgcccgatg
    28081 ccgtgtttga ccgctatcgg gggcatgaaa catcaagcac cgcactactt gccggcgtcg
    28141 accaccgatg cgggcagcgg cgtgcccagc catttctgga acgcggcgct gagcttgccg
    28201 ttcttgatgt tcgccgcgat gaatccgttc atggtcttca gaagctcggg acggttcttc
    28261 tgcatggcga tggccatctg ctgctcgttg acgacataca gcaccttgta cttgccggga
    28321 tagcgcttct gcacgttggc ggcgatcacg gacgaggttc cgaccgcgtc gatctgcccg
    28381 accagcatgg cctgcagggc cgaggcgtca tcgtcgaaac gacgcaggtt ggtgcccttg
    28441 ggcgccacct tggatacgcc cagatcggtg gtgctggcgc gcggcacgcc gatcttgtat
    28501 ttcaggaaat cctgcgggct gctgatgtgc acgctgtccg gcccgagcaa taccatgctg
    28561 gcggccgaat agggctgcga gaactgcacc tgtttctggc gttccggtgt gatggccagc
    28621 gaagccacca ggacatctgc cttgtcggtc aacaggaacg gaacgcggtt ggggcccgtc
    28681 atgaccacga gcttcagctt caatcccaga taatcggcaa gcagtttggc cacgtcggcg
    28741 tcgtagccgg cgggcttgtt gtcggcgtcg acgccgtcgt agggcggcca gtccaccagt
    28801 atgccgacgc ggatctcgcc gcgatccttg atggtctgca tgtaattgtc ggcggccgac
    28861 gcggtgccac tggtggcgat catcaacgaa ccgagcaagg gcaacaggca acgcaccaac
    28921 gctttgccca ggaaggtgtt acgcatgcga tatctccgtc atggttggga catgattcga
    28981 gggaatcagg caaaccaccg ccaggagcat cccctcgatt cgttattttt aattaaatat
    29041 aaaatttatt ttcatattta attctatggc gtggttcatt attccatacc attcgccggc
    29101 aatctgccgg ttatcggtag gtgtttaccc tgaaaatctg taatccccgt gggttctcag
    29161 catagttgat gcctaaattg ccttgtattt atttttctaa taaaaattat ttttaataat
    29221 aaatgcaatc ggctttggcg gcaagtacga tggaatgccg tcggtcctga gacacggccg
    29281 atcagccatc caccggcttg gcaacacccc tcctacaaca cggtgcgcac gtgccacagc
    29341 tccggaaaca gcaccacatc cagcatcttg cgcagatagg gcacaccgtt ggtgccgccg
    29401 gtgccctgct tgaagccgat gatgcgttcc accgtggtca cgtggcggaa ccgccactgc
    29461 cggaaggcgt cttccagatc gaccagttcc tcggccatct catagagatc ccaatgatgc
    29521 gacggatcgc gatacacagc caaccaggcc gcctcgacag tggcgtcgtg acgcgtgggc
    29581 tgcgtccagt cgcgcgtcaa gcgcgccgga tcgatcgcaa aaccgcgccg cgccaacagg
    29641 cgcaccacct catcgtagaa cgagggcgcc tccagtgacc ggcgcagatg cgccgcgatc
    29701 tcgggccgat gcgcatgcgt cttgagcatg gccacattct tgttgcccag cgtgaactcg
    29761 atctcgcgat actggtgcga ctggaatccg gaggacgacc ccagcgacgg acgcatcgcc
    29821 gaatactccg acggcgtcat ggtggccagc acgctccacg cctgcaccag ctgctccatg
    29881 atgcgtgaca cacgcgccag catcttgaag gcgggcggca ggtcgtcacc acgcaccgcc
    29941 tccagcgcgg cgtgcagctc gtgcagcgcc agcttcatcc atagctcact ggtctgatgc
    30001 tggatgatga acaacatctc gttgtggttg ccagaacgcg ggtgctgcgc ggtcagcacc
    30061 gcatccagcg ccaggtaatc gccgtagctc atcgaatgcg agaaatcgag ccgggcgtca
    30121 taccagccct cgtccttgac gccctgtggc ccggtgccgg gagtggattc tttcgtcatg
    30181 atttcatgtg tggtgtgagt tggttcaggt ggtcaaattc tggtgcatca ggtcaccgcg
    30241 cctctcgcct ggaattcggg gcgatcccag gcgcccgagg cgagaatttc gcgcagtgcc
    30301 tcgacggcat cccagacatc gacatatcgg atgtagagcg gggtgaagcc gaaacgcatg
    30361 atgcgcggtt cgcggtagtc gccgatcaca ccgcgtgcga tgagcgcctg catcaccgcg
    30421 taaccctgcg gatgctcgaa cgacacgtgg ctgccgcgcg cggcgtgctc gcgtggcgtg
    30481 accagcgtca aggggaagcc cgcgcaacgg ctctcgacca gctcgatgaa caggtcggtc
    30541 agcgccagcg acttggcgcg gatcgccgcc atgtccgtct gtgaaaaaat atcgagcccg
    30601 cactgcacca gtgacagcga caagatcggc tgcgtgccgc acaggtagcg gccgatgccg
    30661 tcgtcgggct ggtagccagg atccatggcg aacggcgcgc ggtggcccca ccagccggac
    30721 aatggctgct cgaagacgtt ctggtgccgg gggttcaccc acacgaacgc cggcgagccc
    30781 ggcccgccat tgagatactt gtaggtgcat ccgaccgcga aatcggcctg cgcgccattc
    30841 agatcgaccg gcaccgcgcc gacggaatgg cacagatccc acaccgccag cgcgccgcac
    30901 cgatgaatgt cggccgtgcg cgcggccatg tccatcatcg cgccagtgcg atagttcacg
    30961 tgcgtgagaa gcaccaccgc cacatcatcg cgcaacgcgt ccggcaacgc cgagggcgca
    31021 tcgatcaggc gcaacgcata gccatcgtcg agcaaccgca tcaacccttc ggcgatatac
    31081 agatcggtcg ggaagttgct gcgctcggac acgatcacct tgcgcgccgg atctgtgtgc
    31141 ttctgcattg ccagggcggc cgccagcacc ttgaacaggt tcgtcgaggt ggtgtccgtc
    31201 accaccacct cgccggcatt ggcgccgacc aggggcgcca gcgcatcgcc cagcctgcgc
    31261 ggcaacgtga accattgcgc gtcattccaa ctgcggatca ggcccgcgcc ccattcttcg
    31321 gcgatgatcc gccgggcacg ctcgtcggcc tggcacggca gcacacccag tgaattgcca
    31381 tccaggtaga tcacgtcggc gggcagggtg aattgcccgc gcagcggcgc cagcgcgtcg
    31441 cgccgatcga gatcaagaca gtcattgcga ttgacaacca tgagaaccgt gcttccttcg
    31501 aatatcaatg aaaaggctcc gacaccacgc cggcatgcgc ctcatcccac gtgccgccta
    31561 agcaccctcc gaacaagcca ctgcgcctgc ttgccgctcg acttattcaa aagttcgtta
    31621 taacgcccgc aacaccgccc ggacgggact ggcgtcgaga ctcgcgaact tcagcggcaa
    31681 tgcaatcaat tcataatcac cctcttcgac ggcgtcgagc accagcccct cgagaatggc
    31741 catgcgcaga tcacgcacac gatgatgcgc atccagcgtc ttcgaggcct cgggatcgag
    31801 cgagggggtg tcgagcccga tgagccgcac accgtgcgcg gccagaacat cgatggtctc
    31861 gggcgcgatg gcgcagaacg cgctgtccca gcgctcgacc ggcgcacgct gataggtgcg
    31921 caacagcacc cggggcggcg tgccgtcgag gcggccgtgc agatgactgg gcaggatcac
    31981 gggcgccgcg ccaatgcaat ggatcacgcg gcatggcccc aggtacggat ccagcggcac
    32041 tgcgccaatt ggcgcaccct cggcgtcgta atgcagcggc gcgtccgcgt gcgcgccagt
    32101 gtgcggcgac aacgtgacac gcccgacgtt gaccggacac ccgggtccga tgtgccagac
    32161 gcgctcctcg gccactggcg tgtcgccagg ccacgtgggc gtgcccaggc ctatggccgg
    32221 gctgatgtcc cagattcgac ggttgtcatt catgacgggt ctgtctcaaa acatggaacg
    32281 caccgggttg atgctcgaaa ccttagggga atgcgccagg gtacccagaa catgcgccag
    32341 cttctcatat tcgggcaatg cgttgtacac atgcgcgctg agccggatca acccggcacc
    32401 gccccacacc ttgatcacgg tttccagcgc gaattcgtgc gccagccggg cctgaagcgc
    32461 cctggccccg tcagcgcttg acaccagctc cgggggcaac ggcagcgcgc gcatactgag
    32521 ccgaggcgat ccccataact cacgttccgg aatccccagt tcggcggcca ccgcgtgttg
    32581 ccctgcggcg cacaacgcgg cgttgtgccg acgaaccttg tcccagccca gcccacccag
    32641 caaggccaac gcggccggcg cggccagcca ggcggtgaaa tccgccgtgc cggtgtagtc
    32701 gaagttacgc ggaaacgcct ccccgtcttt ggtgcccacg gccagcgtgc gaaacccggc
    32761 gcggaacggt tccgcgacat acagcaccgc ggtcccgtgc ggggcgcaac accacttgtg
    32821 cagattgccc acccagaaat ccggatcgag cgcgtcgagg tccacgtcga gcatgccggg
    32881 cgcgtgcgcg ccgtccacca gcaccggaac acccctggaa tgcgccagcg ccagaatggc
    32941 ggcaatgggg aacagcatcg cggtgtcgga tgtgatctgg tcaaccacga tgagccgggt
    33001 tttgccggtg atggccttgt cgaaggtgcc gaggatttca tcatcggccg cgccggcgcg
    33061 cacatccacg gtgacaagcg ccccgccggc gtcctgacac cggcgctggg cggcgaaccg
    33121 caccgcgccg taggtgtggt tcgtgatgat gatctcatca ccgggcgcca accccaggtt
    33181 cgccagcacc gtggacgccc cggcgctggc attggcgacc aacgcgatgg cgtcggccga
    33241 tgcgcctaga aaccccgcga tggccgtccg cgccttgacc acttcgtcgg gcatccgacg
    33301 cctgaaccag cgctccgggt tgctgtcgac ctccgcgcgg aacgcgtcct ggacctcccg
    33361 gacgtggatc ggcacggcac cgtaggaacc atgattgaga tgggccgccc cagagtcgag
    33421 agaccatagc cgcctgacat cacgccaacc tgagccccac agggggttgt ctgatccggt
    33481 atcgctcatt gtctgtccat tgcccgccat cattgccaca cacgccaccc gggtgcggga
    33541 cataaacccg cgctactccc cgctctggat gtcgcccaca tgcagggcga ggcgccgcag
    33601 cccgtaacac aacaaaatgt agaccacgcc gacgaccaga taagactgca ggaaattgtt
    33661 cgtgtacgcg ccaatgacct cggactggcg cagcagctct tcatagccga tgaaaaagcc
    33721 cagcgaactg tccttcaaca gcacgacaaa ctgcgcgatg gtggagggca gcatgcgccg
    33781 gaacgcctgc ggccagatca cgaaccgcat tctcacccaa tagttcatgc ccaacgacgc
    33841 ggcggcctgg aactggccgc gccccagcga cttgatgcca gccttgaaga tattgctgaa
    33901 tgccgccgag ttgtaggcgg cactgcccag gaccagcgcc cagaacgcgc tgatctcaaa
    33961 tccgagcggg ggcagcccga aataggcgaa cagcaccagg agcagcgtcg gcagcgaccg
    34021 gaagccctcg gcgtacagca ccgatggcca gcgcagacac ttgtgcgtcg acacctggcc
    34081 caacgcgaca agcgccccga ttgaaatggc gatcacggcg tccaccaccg cgaccttcaa
    34141 ggtgttgacc aggccgccaa gaagaaaacg ccaagtcggc cacacgccga agagcgccca
    34201 cttcgaaggt gccagctccc ccttcgcata caagccgtag accagcaagg cgacgcccgc
    34261 ggcgatcacc agcgcgatca ccatcgacag gcgccgcagc ttccgtttgc ccagggggcc
    34321 gatgtctttg acgagcgtgt cgtaggttgt catcggatga tggtgtgggt tttctcgagc
    34381 ctgcggaaga aaacgctggt ggggacggtc agcaccagat agcagacagc ggccgccagg
    34441 aacaggggga tgggttgggc gaacttggaa atgagcgtcg tggcggtgcc ggtgatctcc
    34501 atgacggaaa tcgtgtacgc gatcgaggtg tccttgatga gatcgacaga caggttgccg
    34561 agtgtcggga tcaccgaccg cacggcctgg ggcagcacga cgaaccgcag cacctcgagt
    34621 tgcgtgaggc cgatcgccct agccgcctcg atatgcccca ccgcgaccga gttcacccct
    34681 gatcgaacgg cctccgccac ataggacgcc atgtacatcg acaggcccac cgacgccgac
    34741 acgaacggcg acagcgaaat acccatgtcg ggcagcgcga agaagatgat cacgaatatg
    34801 acggggatgg gcgtgcttct caggacctcg atatagaacg tggccagttt ctgcagggcg
    34861 ccaacggggc tgacgcgcat gacgccgacg atcaacccca ggatgccagc cccgacaata
    34921 cacacggcag agagttccag cgtgatgagg atgccatgca gaatggcatc cagattaccg
    34981 atgatgacgt tagtcacaat agtgcatgtc cgcggcggtc gggggctcgg ggatggtgct
    35041 gaccaccttg ccgatggtgt cggcgaacgc cttcttccac gaaccgtcct ggaacgccac
    35101 caacagctct ttgttcaccc actcgcaaag atccttcgac gatttcttga cgccgatgcc
    35161 gtacggctgc ttttcgccgt tcggattcgc cacgaagcgg aatttgtggg gctctttctt
    35221 gaggaagccg aagatgatga cgtcatccgt cgcataggtg tcgacgcggc cttgctccaa
    35281 tgccttgacg cactgggcgt tggaggtcag gtgaatgatc ttcgcgttgg gcgccttctt
    35341 ggaaagaaga ttcgactcaa tggaattcgt cacggcacag acactatgcc cgttcaatgc
    35401 ctggtagccc ggtatggagt cacccaggcc cttgtagtct ttgcggacca tgatggccgg
    35461 ggtggacaca tagatggggc ccgcgaatga aatcgccttc tcgcgctcgg gcgtgatggt
    35521 gtaggtggag ctgactaggt cagcccggcc ctgctgcacg atggtttcac gcgatggccc
    35581 gggcgcctcg acgaattcca catggccgtc ggccccgaac atgtcccggc ccagtatctt
    35641 ggcgagctcg ggctcgaagc cctcgaccgc gccggtaatg ggattcttct ggccgaaagt
    35701 gggcacgtcg tacttcacgc ccacgaccag cttgcccctg gcctggatct tggccatggt
    35761 ggagtctttc gggaaggtgg gcgcggcccc ggtggaagca ccgtacgcca cacccatgcc
    35821 gatcaggccg ctgcacgata gggcaaccag cacggcggac aggcgcacgg cccgggacgg
    35881 gccgcgccat aagatgtgtt tcattttcga gtctccttgt gattggtttt ttaactgcgt
    35941 gccacccatg aatgctatcc gtggcgctcg aatgaggcaa ccggatagga catgatctga
    36001 gacaggaacc gcgccgcgcg ttcggtcttg ggttgcgtga agaattgcgc aggcgttcct
    36061 tcctcgacga tccggccatc gaccatgaat acgatccgat cggcgacttc ccgtgcgaaa
    36121 tggacctcat gcgtgaccag gatcatggtc atgccgtcgg cggcaagcgc tttcatgatg
    36181 tccaggatgc cttggacaag ttctggatcg agcgcggagg tgggctcgtc gaacagcatc
    36241 aggcgcggcc ccatcgccag ggcgcgggcg atcgcggcgc gctgctgctg cccacccgag
    36301 aggttggccg gatacttgtc cgccagcccg tctatcccgg ttttcgccag caggcgcagc
    36361 gccagctcgg tcgcctgggc gggtgtctgc ttgcgcaccc gcaccggccc caggatgacg
    36421 ttctccagaa ccgtcatgtg cgggaagagg ttgaaggact ggaacaccat gccgagatcc
    36481 gctcgaagca gggctagatc cttcccttcc cgcggaatct cggtgccttc gatgtggatg
    36541 gtgccctcat cgatatgctc gaggcgattg atgcagcggc agagcgtgga cttgccggac
    36601 ccggacgggc ccagtatggc gacgacctcg ccacggtaaa catccagatt gatatcggtg
    36661 agcgccgcca cggcaccaaa ccgcttggtc gcatggcgca cgctcacgac aagggcgcgg
    36721 tcatcggccg atgccggcgt gtccaccgaa gcggcctcct tcacaatagc accggcctgt
    36781 cgtccagcgc attgatgccg ccgccctgaa cgccaggcag gcgggggcag tgctccgcca
    36841 gcttcgcctc gatggcatcg atcgcggcca gcaccccgtc ggtgtagcgc ccgaactgaa
    36901 ggtcggtgcg caggttggcg cacacctggg cccattggtc atcgggaacc ggaacgccgc
    36961 gatcggccac cagctcgatg ctgtgccggt ccagcgccat gtaaagcagg acaccggaat
    37021 tgccgggtgt atcccacacg cgcaggcgac cgaacacctc cagggcgcgc aaccgcgtgt
    37081 cgcgctcatg ccccggcgac accgcctcga tggccagcat caactccccg ggatgcgccc
    37141 gctcgccctc ggcgatgcgc cgggccactt catcaagaaa gggcttcgtg aaatgtttgc
    37201 gccgcagcca atggccttcc acgaaggccc aaccggtcca ttccttccac ttgccgccat
    37261 catgccgcac gccagttctc ctgtatgccg gctagcgtag cgtttgacgc tatctggaac
    37321 ataaaaacgt caaacactgc gctagacccc ggcgttgttt cccgctttgg gtctcatgcc
    37381 cccaccgcac gggcggatga ctgccttcgg caccgccatg cccttcacca gccgcccgaa
    37441 gccccgcccc cgccgctgcc tccgccgccg ccaccgaaac cgcctccacc acccaggccc
    37501 ccaccgccgc caaaaccgcc ccccattcca cccccccagc cgccgacgat gccgcccagc
    37561 atggcgccat tgcgcgaggc ccgcaatgaa tttcgacgtc caccggcacg cgccgcccgc
    37621 cccgccaggg ccagcacaaa gccgaccatc gcgcccaggc aggccaggaa cacactgcca
    37681 aacatgaaaa agacaaacaa ggccgccgcg aacgccgcac ccgccggggg caggacgaac
    37741 gccatgaaca ccagcggcaa caacatgggc aagggcgcgg agccggtatc ctgccgcgcg
    37801 gtctgtttca ccggcggcaa ggtttcgcca ttgaccagtt tcaccaggct gtcgacgccc
    37861 gcgctcaccc cttggtcgaa atctcccttg cggaatgccg gagccacctg ttcacggatg
    37921 atgcgcccgg ccaggatgtc gggcaccgcc ccctccaggc cgtatcccac ctcgatgcgc
    37981 atcgtgtggt ccttcttggc gatgacaaac aagatgccgt catcgacatt cttgcggccc
    38041 agcttccatt ggtcgaaaac ccgtctggcg taatgttcga tggtgtcgcc gtcggtggac
    38101 ggcaccatca acaccgcgat ctgagcccct tttttctttt caagcccggc cagcgtggcc
    38161 gtcaggcggg tgaccgtatc ggcgctcagc gtgtgggtga ggtcggtgac acgttgcgtc
    38221 aggaccggca ctttcgcttc gtccgcccgc gcaacctggg cgaacgacgc caacagcgcc
    38281 aggcacagtg ccatgcaggc caaccatccg cggcgcatgt tatttggccc cggcgcccgc
    38341 cgcgcccgga atgtcgaact tgacctcggg atcgcgcatg agcttgtcct gctgatcgac
    38401 gccatagttc tccatcgtgt ggtaaccaaa cagcttcgcc gtgaccaatg ttggaaactg
    38461 acggattgcc aggttgtacg cctgcaccgc tttcacgtag cgcccgcgtt cggtggcgat
    38521 gcggttctcg gtgccttcca gttgcgtcat gaggtcgcgg aacaggccgt cgctcttgag
    38581 ctgggggtag ttttcagaca cggccaacag gcgcgacagc gccgaagaca attgcccctg
    38641 cgccgcctcg aatttcttca tcagggcggg gttgtccagg tctttggcac tgatctggat
    38701 actgcccaca cgcgaccgcg cgtcggtgac ttgttccagg acggtgcgct cgttgaccat
    38761 ataggcattg accgcgctga ccagcttggg cacaagctcg gcacggcgct gatactggtt
    38821 cagcgtttgc gaccaggacg ccttgacctg ttcgtccaac cgttgaatgt cgttgtagcc
    38881 gcagccagag agcatcaagg tcaggcaggg tagaagcagg aacagcagga aacgtttcat
    38941 gggtttcatg ggccaccgga ttttctgaaa attggaaccg gcgcgatgcg ccgtgcctgg
    39001 cgacgatttc gacttgtttt tcattcaacg aacttacttg tgagacacaa cacgggcttc
    39061 gcgaaacaat tttcgcacag aacacacacc gctccgcaac agaattccct ggttcagcac
    39121 actgtccggc tgcctcggcc acccgacttc ggacccatgc accaaccgcg cggcacacac
    39181 ctccgcggtt cccgcgcaac agcccaccgt ccgctcagcc aactctcccc acaactgggt
    39241 tacaatcgcc tgctgtgcat tctgccagtc aatgcaccga cctgttggcg ctccgcctgc
    39301 ttgtttcccc tctatttcct gttcgtctgc ctagattggt ctctctcgac tcgagcgata
    39361 ggctgtcgct ggagttttca ttgaataccc acgtttcttt tgcctccctc gggttggcgg
    39421 aacccctttt gcgtgctgtc gccgacaccg gctacaccca ccccaccccc atccaggccc
    39481 aggccatccc gcaagtcatg aagggcggcg atctcctggc cgccgcccag accggcaccg
    39541 gcaagaccgc tggcttcacc ctgcccgtcc tgcatcgcct gacgcaatcc acgccggccg
    39601 ccctgcgccc gggccggccc cgttgcctga tcctgacccc gacgcgcgaa ctcgccgccc
    39661 aggtcgagga atccgtgcgc acctacggca aacacacctc ggtgcgttcg ctggtcatgt
    39721 tcggtggggt gaacatcaac ccccagatca gtgccttgcg caagccggtc gacatcctgg
    39781 tcgccacccc ggggcgcctg ctcgaccacg tccagcaaaa aacgctggac ctgtccggcg
    39841 tggaaatcct ggtgctggac gaagccgatc gcatgctcga catgggcttc atccgcgaca
    39901 tacgcaaagt attggcgttg ctgcccaaga cccggcagaa cctgctgttc tcggccacgt
    39961 tctccgacga tatccgcacc ctgtcgcgca cggtgctgaa caaccccgag gaaatctcgg
    40021 tggcgcgctg caacaccacc tccgacctgg tctcgcagcg cgtcgtgctg accgagcccg
    40081 ggcacaagcg ggatttgctc agcttcatca tccgcgaaag tggctggcac caagtgttgg
    40141 tgttcacccg caccaagcac ggcgccaacc gcctggccga gaagctcgtg aaggatgggc
    40201 tggtcgccgc cgcgatccat ggcaacaaga gccaggcggc gcgcacacgc gcgctgagcg
    40261 gtttcaagga aggcacggtg gccgtcctgg tcgccaccga catcgccgcg cggggtctgg
    40321 acatcgacca cctgccgaac gtcgtcaact tcgaattgcc caacgtaccc gaggattacg
    40381 tgcatcgcat cggccgcacg ggtcgcgcgg gcgtcgaagg caacgcgctg tcgctggttg
    40441 accacaccga aatgaaatac ctgtcggcca tcgagaagct catcaagacc tccatcgaac
    40501 gcaccgccat agaaggctgg acgcccgcca tggtgaaggc cgaaccgcct cgatccgacg
    40561 cacggcccgc gcaggcgccg ggccagcggc gcagcccgcg caaaccggaa accggcggcg
    40621 cgccgacccg atcgggccag gcgcggcgca acaccggccg cggcgacgcg ccggcgcgtc
    40681 acgccgaggg caatgcccgc cgtgctgaag gaaacccccg ccgtgcagaa ggaagcgccc
    40741 gccgcaccga gggaagcccc cgtcatgccg aaggcagtgc gcgtcgcgcg gaaggcgccg
    40801 tccgccatgc cccacgcggc gccgatgccc ggccacgcac gggcgcgccc cggcgtgacg
    40861 cctccggcca atcgcgcccg gttctgctga gcaagtaacc ctcccttcca ccacgaggcc
    40921 ggcccatcat gaccctctcc acctggttgg cgttcttcgc ggcatcctgg gccatttcgt
    40981 tctcgccggg cccgggcgcc atctccgcca tggcctccgg cctgaaacac ggctttcggc
    41041 gcggctactg gaccaccatc ggcctgatcc tgggcatcat ctgccaggtc atcgtcgtgg
    41101 gtgtgggcct gggcgccctg atcgcggcgt ccgagagcgt gttttccgtg gtgaaatggc
    41161 tgggcgtcgc ctacctgatc tacctgggct ggaaacagtt ccgcaccgac gcttctcctg
    41221 tcgcggtgga atccaaccgg gcgtcgcact tcagcatccg cgagctggtc acccacggct
    41281 ggctggtgaa catcaccaac ccgaagggca cggtgttcct gttggcggtg gtgccacaat
    41341 tcctggactt gtcgcatcct ctcgcggccc aatacctggc gatcggcgcg accttgtgct
    41401 tcaccgacct gttcgccatg gggttctaca cggcactgtc gtcacgcctg ctcaaggtgc
    41461 tgcgcaaccc ccgacacatt cgctggatga accggacctt tggtgcgctc ttcatcctgg
    41521 ccggcacggt gctggcctct ttcagccatc atcgttgacc ggtccggccg cgccggcgcg
    41581 ccggcccccg ttacaaatcc gccccatgag ccttacgcac gaagtcgaac ggcgacgcac
    41641 attcgccatc atttcccacc cggacgccgg caagaccacc ctgaccgaaa agctgctgct
    41701 gttcgcgggc gcgatccaga tcgcgggcag cgtcaaagcc cggaaagcca gccgccacgc
    41761 ctcctccgac tggatggaaa tcgaaaagca gcgcggcatc tcggtggcct cgtcggtcat
    41821 gcagatggag taccgcgact gcgtgatcaa cctgctcgac accccggggc accaggattt
    41881 ctccgaagac acgtaccggg tgctcacggc ggtcgacgcg gcgctgatgg tgatcgatgc
    41941 cgccaacggt gtcgagccac agaccatccg tctgctgcaa gtctgccgcg cccgcaacac
    42001 ccccatcatc accttcgtga acaagctcga ccgcgaggtg cgcgagccgc tggacctgct
    42061 ctcggaaatc gaatcgcatc tcggcatgga cgcggtgcca ttctcgtggc cggtcggcat
    42121 gggccgcacc ttcggcggcg tgttcgacat cggtcatgac cgcatgcgcg tgttccgcgc
    42181 cgggcacgac cgccgggacg accacaacga gttcatcgag gggttggaca accccgaggc
    42241 cgcgcaacgt ttcggcgacg ccttctcacg ggcgcgcgac gacatcggcc tgctgcaggc
    42301 cgccgccccc gtcttcgacc agcaggcatt tctcgacggt cgccagacgc cggtgttctt
    42361 cggctcggcc atcaacaatt tcggggtgca agaggtgctg gacgcgttgg tcgatctggc
    42421 ccccaggccg ggcccgcgcc aggcgctgga acgtgaggtg cgccccgaag aacccaaatt
    42481 cagcggcgtg gtgttcaagg tgcaggccaa catggatccc gcgcaccgcg accgcgtcgc
    42541 gttcgtgcgc gtcagctcgg gcaggttcga acgcggcatg cggctgatgg tggcccgcaa
    42601 tggcaaggaa atccggccga acaacgtcgt gtcgttcctg tcgcagcgcc gcgaactgct
    42661 ggatgaggcc tacgccggcg acatcatcgg cattcccaac cacggcatcc tgcaattggg
    42721 cgatgcccta acggaaggcg aaagcctgca tttcacgggc ctgccgttct tcgcgccaga
    42781 actcttcagg agtgtggagg tcaaagatcc tctacgcacc aaacaactgc gcaccggcct
    42841 gacacagctc ggcgaggaag gcgccatcca ggtcttccgc ccggaaatca cgggaggcgc
    42901 gctgttgctg ggtgcggtcg gccagttgca gttcgaggtg gtcgcccatc gcctgctgac
    42961 cgaatacaag gtggatgcgc gcctggcgcc atcgcgttac acaatggcac gatggattac
    43021 atcgaaagat cccaaagcca tgcgtaaatt cacccaggcc aacgcggcca acatcgcgta
    43081 cgacgtggtc gacgccgtgg ccttcctggc cagctcgccc gcgcagttgc gcgtggccca
    43141 ggaactctac cccgacattg agttccacgc gatgcgcgaa catggcggcc acgttttcag
    43201 ccagggcacc gcggcgggct gagacggcgt ggcgtccgac tcgttctata ctatgaatcc
    43261 gtttttgcca acgatcgccg cgcccgccct tcgtggcggc gccccgaacc gaggacctgt
    43321 atgtcttggc gttttattat cgcagtgctg ttgattgccg cgggcgcatc cgcctggggc
    43381 ggcctgcggc tgggcaactg gctcatcgcc cacggcccgg agaaggcaat cctccataac
    43441 gaaccggcca tgcccaaggt tccggtcctg gacgccaatg ggcacgcgta ttcggcgcag
    43501 ccgccgcagc ccctggctga cgggcaactg ggttatcaac agcccatccc ccctgtcgac
    43561 tggcacgtcc aacaagccga cgcggccacc gcgcagccga acctgaacat cgccatggcc
    43621 accaccacca tcaccatgga ccaggccaag ctcatcgccg cgggtggtgg cagcacccag
    43681 ggcgccaacc tgtcgggcat cgccgatgtc agcggcctgg tgggcggcaa gccggccaag
    43741 gcgccgctgc aacccgtgga cgtgcccccg cccccgccgc cgcccgatca aatgcccacc
    43801 cccaatacca accggaactg gcaggcccgg ttccacaagg cgctggaaat ctgcagcaag
    43861 cagggcttct tcgaccgccc cagctgcgcc tgggcggccc gcaaccaata ttgcgagccc
    43921 aaccacgcct ggggcaaggt gcgcgactgc cccgccaaga aagagttctg atcggaacgc
    43981 ggcaccgcca acccaggaca tcaacggcgg agcaaagccc cccgggccgg ccccccccgg
    44041 caggggagcc caagcagaca cccctttgaa acacacgcgc cccggtcaac ggggcgtcgt
    44101 gattcaggca gcggcgaacc gccagacatg cggcatcgac cgactactcg gcgccctcgc
    44161 caatctgcgt ctgcaggtaa ttctgctcac ccaccttgcc caccagttcg atctgcgtct
    44221 cgagccagtc gatgtgctct tcggtgtcgt tcagaatgtc ctggaacaga tcgcgcgaca
    44281 cataatcgtg aaccgactcg caatacgcca tgccttgctt gaccgtgtcc tgcgcggcgg
    44341 actcgagctt caggtcgcag gccaggattt cgggtacgtt ctcgccaatc atcagcttgt
    44401 gcagatcctg cagattcggc aggccatcga gcatgaggat gcgctggatc agccggtcgg
    44461 cgtgcttcat ctcgccaatc gactcttcat gttcgtgctt gcccatgcgg tcgaaccccc
    44521 aatggttcag catgcgcgca tgcaggaaat actggttgat ggccgagagt tcgttcttga
    44581 gctgcttgtt caggtgctgg atgacggttt tgtcgccttt catggctgcc cccacagtgg
    44641 ttcatggaag tgccgcgtca tgccgtggcc gcgcggcgtc gtgtggcgat agcctaccat
    44701 gccccccggc gccattcacc accttgcggg acctgcgccc caccggcacg cggatcgtaa
    44761 gccgaaacgg ggtgagaatg tgaatcagac accgtacaac cctcagtcat cgaagaaaga
    44821 tgccaacacg gggcttcgac aacgcgccac ccgacccgac ggacggtatc cggccgcgca
    44881 attccacgcg aaccgcgcga tccggcgaag ggaaaacggt gtcgtgacgc gcacagcgat
    44941 attgttatta tttgtatcta tcgatcctac gtccaaacac ttgcccttgc gccttgaggt
    45001 gaagattcgt cgcgccacaa atcagccgat atcgagtccg gcaagggttc acagaggggg
    45061 aaggctggaa cgcgcctgac gacgcgtcag atgcgacggg ccgcgagcgc acacccgcgg
    45121 tactagggtg ggggccgcga tgacgcgtca ggcgcgacgg gccgctatgg cgaccatggg
    45181 gacaatttcc gtgtcggcgg aggcgtcgtt ggccgccacg accggcgcga cggatgtttc
    45241 gtgcgacacc tggccggcat agcgcccgcc gggcaggtat tcggtggcgg tttccgagca
    45301 acagccacag catgccccga cccccagttg cgactggaga tcggacagag aacgggcgcc
    45361 cgccgtgacg gcggaccgga cctggttctc ggaaatggcg ttacagacac aaatgaacat
    45421 gagaataatt atcatctaat ttttctctga aagcaatccg tgtgacgcaa ccgcaacatc
    45481 acacacaatc gcgggccact cgcctcgtgt cggggtcacc accgcacagc gccgccctgg
    45541 ccttgcgcac actggccgct tcgatgccgg cggcttcgcg gtatttcgcc acggtgcggc
    45601 gcgcgaccac cacgccctgg tctgacaagc gggccgcgat ctggctgtcg gacaagggtt
    45661 tcttgcccga ttcctcgctc accagcttga tgatcagtga gcgcacggcc tcgcccgaag
    45721 tggtaccgcc gtcatcggtg gccagcgccg cgccgaaaaa ccgcttgagc tcaacgaccc
    45781 cccatggcgt ttgcgcgtat ttctgtcgtg tggcgcgcga cacggtggat tcatgcatat
    45841 ccagttcctg tgccacgtcg cgcaacagca gcgggcgcat ggcgcccatg cctcgttcga
    45901 aaaaggcttc ctggcgactg acgatgacct gcgtcacccg gagcaccgtg ctgaagcgct
    45961 gattcacgcc cttgatgaga ttgtgggcct gctgcacctg ggcctgcagc gcacgcgggt
    46021 cggggccatc gccgagcaag cgctcgtaca gaggattgac gcgcaagcgc ggcgcgagcg
    46081 ccgggttctg gaagacccgc caccccccct tcaccttgcg cacaagcacg tccggcacga
    46141 cgaaatccgc caccgacgtc gcccacgccc ggcctggccg ggggtcgagc cgcagcagca
    46201 aggcatgcgc cgcctggaac gtgtcgtgcg agcacgacag cacatcgcac aggcgattga
    46261 cgctgccggc ggcgagcaga ccgagatgat cgcgggcgat gtcacgcgca catgccagga
    46321 cgccgggcga tgcgtcggcg cggcgcgcat cgagctgcag cagcaggcat tcggacaggg
    46381 cgcccgcccc cacacccgcc ggatcgaacg actgcaacag gcgcaccgcg gcgcgccatt
    46441 cgtccgcgtc ggcggccacg tcggggaatg cggtggccac ctcggccagc gacactggca
    46501 aataaccgtt ttcgtcgagc tcgccgatca agatgccgac caggaggcag tcacgcggcg
    46561 tggcgcgcgt caggcgcaac tggccaagaa ggtggtcgga aagcgtttcg tcggcactgg
    46621 tttcgacgat gccgtcgcca tcgccgtcac cgcgccgggc ggtgaccgac ggcgtggacc
    46681 agtacagcgg ctcggtttcg gcgtcccggg gttcttcccc ctggggcgct tcgatgtcat
    46741 actcttcctg acgttcgagc aacgggtttt cctggaccgc ctgggcaatc tcggcctcca
    46801 ggtcctgcgc cgacaactgc aacagccgga tcgactgttg cagctggggc gtcagcgcca
    46861 actgctggcg ctgtttcaat tccaggaatg gtccaggctg cataggtaaa atagtggcat
    46921 gaaacaaaac tcacccaacg aacccacccg ccaggacggc gccctgctgc gcaacacctt
    46981 gttgaaactg accgcggcac agaaaatcct gtgcgtggtc gtcgtcgtcg tggtggctct
    47041 ggtctggtac gacctgctga accagctcgt ggcgttcgga cacaacatcg actattccgg
    47101 gctgcatgcg ctgggcaacg acatggtgca gatgctcaag ctgtacaacc cgtatttctg
    47161 gtggggggtg gtcgcgctat gtaccctcat tattgcctat ttcctgcatc tgttcgtgca
    47221 ggccacccgg cggcgcgtgc gcatgcggct ggtcagcgcc gacgtcatcc acacgcttgc
    47281 gcgtgaactg tcgcaaccgg ccaaagacgt cctcctgtgg gtatggcaag accgccacga
    47341 ccccatcacc gtaggtgatc tgcaacgcac gtccgacgag ctccacgcgc gccgcgccga
    47401 caagatccgg ctggcccagg atcacgcctc ggccctgctc ggcgaacaaa acggcgcctg
    47461 agcggcgcag cggcccgctg tactgaaccg ttcccgccga acgccgccat tgccgggccg
    47521 caatcacgaa ccgcttgcct tctcggcgaa aatgtgttcg actattgggc atgagttccc
    47581 cacacgacgc ttcccgcaac acttgcaacc gcctcgggca ccccgcccgc cgcgttgcgt
    47641 cgtgcctcgt gcttcccctc gccctatccc tgctgctacc gatcggttgc cgagcctagc
    47701 gtcgcgtggc agttcggcag cccgtgcaag ccacccccga tatccccgtt cgtagttcgc
    47761 cccctcccac ttgcgtctga accggcctca agcgccgacc cggcgaacgc gatccgcaaa
    47821 gctccagccc aaaaggtacc ctcgatgatt cccgtcccgt ccgacaaata ccggccgttc
    47881 gtgccgttca cccgcgactt ctccgaacgc acctggccca gcaagcgcat cacccatccc
    47941 cccatctgga tgagcaccga cctgcgcgat ggcaaccagt ccctcatcga acccatgaac
    48001 gtgaagcgca agctgcgctt cttcgagcaa ctggtcaaga tcggtttcaa ggaaatcgag
    48061 gtcgggttcc cgtcggcgtc gcagaccgac ttcgatttcg tgcgcaagct ggtcgatgac
    48121 aagcgcatcc ccgaagacgt caccgtcatc gtgctgaccc aggcgcgcga agacctgatc
    48181 gcgcgcaccg tcgaatcggt ggcgggcgcg aaacaagcca tcgtgcacat gtacaacgcg
    48241 tgcgcgccgg ggttccgcaa gatcgtgttc aacatgaccc aggacgaggt caagaagatc
    48301 gccaccgatg gcaccaggct ggtcaagcac tacacctcgc agcacccgga aacgtcgtgg
    48361 cgctacgaat actcccccga agtgttcagc acgaccgaac ccgaattcgc gctggacgtg
    48421 tgcaacgcag tgggcaaagt ctggcagccc acgccggaat cgaagatcat cttcaatctg
    48481 ccggccaccg tcgaggccac gacgccgaac atgtacgccg accagatcga atggatgcac
    48541 acgcacctgg acaatcgcga ctgcgtggtg ctgagcctgc atccgcacaa tgaccgtggc
    48601 acggccgtcg ccgcggccga actggccatc atggcgggcg ccgaccgcgt cgaaggctgc
    48661 ctgttcggaa acggcgaacg cacgggcaac gtcgacctgg tcacgctggc cctgaacctg
    48721 tacacccagg gtatccatcc cggcctggat ttctccgata tcgacgaagt gcgccgctgt
    48781 gtcgaatact gcaatcagtt gccggtgcac ccccgccacc cgtacgtggg tgatctggtg
    48841 ttcacggcgt tctccgggtc gcatcaggac gccatcaaaa aaggcttcgc gcgccaggat
    48901 ccgaacgcgc tgtgggaagt cccctatctg cccatcgatc ccgccgatct gggccgcagc
    48961 tacgacgcgg tcatccgcgt caacagtcag tcgggcaagg gcggggtgtc gtacctgctg
    49021 gaatccgaac acggtctggt gctgccgcga cgcctgcaga tcgaattcag ccgcgccatt
    49081 caacgcgtca ccgacgaaac cggcaaggaa gtcacggccg aggccgtcta cgacatcttc
    49141 agcacggaat acctgaagca ggaacatccc ctcaagctgg tgcgccaccg catcagcggc
    49201 gaccccaagg ccgcggctgg ccagcagttc cagatcgagg cggagatcga ggaaaacggc
    49261 caggtgcgaa acctgacggg caagggcgac ggtgcgatcg cggcgttcgt caatgcgctg
    49321 aagctgccca tccgcatcat ggactacagc gaacacgcca tcggcaccgg caccgacaca
    49381 cgcgccgcgt cgtatgtgga gctgcgcatc ggggagtccc cgtcgggctt cggcgtgggc
    49441 atccacggcg atatcgtcac cgcatcgttc ctggccatac tcagcgccgt gaaccggcac
    49501 atgatcagcc agacccagga ggccatggcc gcctgaaaaa acaagcgccc ccggggggcg
    49561 ctccgaaaaa aagccggctg tgctcaaaag gcacagccgg ctttttcatg gatcggcgcg
    49621 ggtcagaagc tgacattggg ccgcgtgggc acgcggcctt ttctgccggc cgggatgaca
    49681 ccgaccggca ccgggacgat gccgctcacc aggacgaagg gccgggctcc caggtgacat
    49741 cgacgttatg ttcgcgcgac aggcgcagca tcgcgagcaa cgggaaggcg cgccggcgga
    49801 agctcacagc ctgcgagatg gggtggatct cggaggcgat ctggtcgctt tcgtcggaga
    49861 gttccggctc tttgtcggcg gcgatggcat gttcgatgcc ggcgatggcc gcatcgagct
    49921 ggtcacgggt gatgacaccg cgttcgggca gcgtatcggt ataaggctta cccgccgcct
    49981 tgagtatcgg cagcgcgtgc ttcgcgaaca tcaggacttc cgcggctgct ttggaataaa
    50041 agacaactag catattgtcg gtttccctca atggacatat caatacacta ctgtaaccca
    50101 gggaatcaag caagcgtcag actgtgagcc atcccttaca tcaagagcct cgtcatgacg
    50161 cgacccgtgc ggctgtccat catcaccgtg tgcctgccgg tcgcgctgat ggtcggcacg
    50221 ccgggccacg ggcagccaac gtgcgggcca ccctcgctgg gttcgccttg cgcgctgggc
    50281 ggcccggccc tgctgccccc caacgagccc tcgttgaatc tgggcgccgg caaccccatc
    50341 catttggcga ctggcaacaa acaccagcgc gaaaccgatc tgcccgccat cgccggcgtg
    50401 cccggcctcg aaatcgtccg ccactacaat tcccgcgatc atcgccagtc cgcattcggg
    50461 tctggttggt cgctgtccta cgacgcccgt ctgtatcaca tcggggccac ctggcagatc
    50521 gcccagcccg acggcagccg catcctgttc cagggtggtc cggacggccc catgccgaat
    50581 cgacacgggc gcctggaatc gcaccaatcc gggtggcgct ggacctggcc gaacgggcgc
    50641 gagttgcggt tcgacacgca cggcaggctg atcgccattc gcctggcggc gcgccacgcg
    50701 ctcacgctgg cgcgccacga gggcccggaa tcattgcgcg gcgccatcgc atccgtgacg
    50761 cacaacggtg gggccaaatt gacgctgcat tatctcgtgc gtggcggcca tgcctacgtg
    50821 gatcgggtcg gcagccccgt gggggttttc caataccagt acgaacctgt ccctgcctcc
    50881 agcctggatg acggcacggc accgacatgg cggctggtcg gcctgacgcg tccggacggc
    50941 atgcggcgac gctacctgta tgagcccgaa caccaggcgg gcgatccgtt gctgctcacc
    51001 ggcgtggcca tcgtatctgc cgacggacgg cggatggccc gaaccaattc gtgggcctac
    51061 gacaaaacag gacgcgccat ccggtcgaca caaggcgggc cggatgcaac gaaaggccat
    51121 gtgtcgatca cctatgtgcg accgccatcg ccggatcgca cgggcctgac ccgcgtgacc
    51181 gatgccgacg gccagcgcag ccagttcatc accgccgtca aaggcggacg gcatgtgctg
    51241 ctggacgccg agggcgcggg ctgctcgggc tgtcccgcgc cgggcaccca tgcccagtat
    51301 gacgaactgg gccgcctgac cgaggtcaac ggcacgcgca tcgaacgcga cgtgtccggt
    51361 gcaatcacct cggtggcccc gtccggcatg ggttggcccg ccctggcgct gcactaccag
    51421 gccaatgggc gccgggcctc atggacgtcg gccgtgaccg gcaccgaaca tacgcgattc
    51481 gatgccgcgc atcgccccgt gcggcgggag ttcgccaatg gcgatgtctg ggagtatgac
    51541 tacgatgcgc acggccgccc tgtgcggctg cgggaatcga gcgtggaagc gaccggtgcg
    51601 ccaagtacgg caagtacgcc aggcgtggcg gatgcggagg ggacagcgca tccgtcgggc
    51661 atggtctcgc cacaaacgac gacgctgtcc tggcggggcc gcctgctcga acgcgtcacc
    51721 catccgcatg aaaacgaatg gcgcgggtac gatgccggcc aacgcatggt ccggcgtgtc
    51781 gtggagcgcc cgcatggcag cgtgccacaa cgccacatcg acaccttcca ctacgacaac
    51841 cacggcaggc tggcccgcca tgacctgcca gaagggggcg ccttgctcta tcactatgcg
    51901 ccgcgcggcc ggttgctggg catcgactgg ctggatcgcg acgcccgcgc gcacaccgtg
    51961 atcgccacca cggcgggccg gccgggctat cgctacggca atggcctcac gctcacgacc
    52021 cgccgaaccc acggccagga cactggcctg gccgtggccg atgcgaaggg tgttctgctc
    52081 gcggaaagcc ggcgctacga caatctgggc cggctggcac aggaaaccca caccgcgccc
    52141 gcggcgggat tcgacgaaac ctggcgctat gcgtacgacg cacgcggccg catggcgggg
    52201 gcgcggcgtt tcacacgggc cggagccgtg tccgcgcgtg gcaacccgaa tccaaccgac
    52261 gtcatcacag gcaaatccgg cgacaacgcg aaccgaccgc caccttcccg ctccatggcg
    52321 tcagaccctt ccatctggta cgcgtggcgc gacgacggct ccctcgccgc gcggcgtgac
    52381 gggggacgca cacaacgccc caccatcgcg cgggacccat ccggcctacc ccgcgccgtg
    52441 ggcgatatcg tcctggaata cggtccaaac cggcgtcttc gacgcgccgc gcgcggtgcc
    52501 agcaccctgg ccacgtatcg ccacaatgcc ttcgggcagc gcatcacggc cgagtccacc
    52561 ctggccagaa ccgattactt ctatctggac aacaaagtcg tggccgaggc ggccaggccc
    52621 gtcccgcgga cacagccgtc cggccgggca acggccggga cgaccgcacc cgatccaccg
    52681 accatcacgc gacgctatat ctatgcggga gacgtcctgg tcggcctcat cgaccaccca
    52741 ctcgatcagt ccgccacggt cgggacactc tatgccgtcc acaccgattt gctgggcgcc
    52801 ccacgggcgg tgaccgatgc cagccaggcc atccgctggc tggccgccta cacacccacc
    52861 ggcgaggcca gacgcgtcgc gggcgacctc acgctggacg tgcgcctgcc ggggcagatg
    52921 ttcgatgccg ccaccggttg gcacgacaat cttctgcgca cgtacgcccc cgccctgggg
    52981 cagtatctgg agcccgaccc gctcgggccg gttcccggca accaggcgtt cggctacgcc
    53041 gcgcagcgtc cgcgccgcta tacggatcca ctgggcctgc tgctgttcgc cttcgacggc
    53101 acccgcaaca gcccggtgac gcagtccaat gtctggaaaa tggcccgcca atacctcgat
    53161 ggcccggtgt tctatcaggc cggccccggc acgcccgcca atctggattg ggatgccgtg
    53221 acggcccgca gcgcaccgga tattctcgcc gcgcaatggg atgccctgct gcagacactg
    53281 gcacggcccg caacgcggaa cgacaccatc cccatcgaca tcattggttt ctcgcggggc
    53341 gccgccttgg cgcggcactt cgccaaccag atcgccggca acacgcgtga cgggtacttt
    53401 tcctacacgg atgccgagcg cggcctcatc acggcctgcg tcgatctgcg cttcatgggc
    53461 ctgttcgaca cggtggcgca gttcgggctg ctgggttcgc agaacgccaa ctacgacctg
    53521 accatcgcct cggcgtggga gtgggtggcg caggccgtcg cgctccagga gcggcgcagg
    53581 ttcttcccac tggtttcgat caccggccag ggcaacacca tcgaggcgcc ctttatcggc
    53641 gcccacgccg acatcggcgg tggggtgtcc accgacgcca ccggacaggc cacctcgcgc
    53701 ggcgacctgt ccgacgtgac cctgaactgg atgttgtggc aggcgcgcgc ggcgacggcg
    53761 cgctttgcgc cgcccacgtc ggaggacggg gaaatcaccg accccattct gcacgatgag
    53821 cggctgccgt cgtctcgttc cctcgacgat ggagaccggc gcgtcgacgg ccccgacggc
    53881 aagcaagtag tggatcgcca ggacgacctg gcccgtcttg ggcgcgcgcc acgcgccgcg
    53941 accgaggccc tgatcacgcg gaccgacaac tggcggtacc tggacatcgc cgaggtgggc
    54001 acggtggaca tggacggcta cgcgcgctgg ctgcacgatg aactcggctg gcaggcgttg
    54061 cccgcctgat cgggcgccgt gggccgacgt ccagcgcatg gcacagcatc ggggccggcc
    54121 ggcccgtgct ccacagcgag catggtggac ggagccgcaa ggacgctggc tgtggggcgc
    54181 ctcggggcaa atcacgctat gattgtctcc ttcggggccg gctccgcgcc ctgccgtggc
    54241 ccattccccc ggcttttcca ccacgcctac gtactttatg ctccccaggc aacgacaaca
    54301 actcatttcc ctgctccaga ccgctgtcga cagcgtcgtg cccggcgccg cctccgacgt
    54361 cgtgctggaa caaccgaagg tcgccgccca tggcgacatc gcctgcaaca tcgccatgca
    54421 actggcgcga cgcgtcggcc gcaaaccccg cgacatcgcc gaggccatca cgcaggcgct
    54481 gcgcgaccag cccacgctgg ccggcctgat cgcctccatc gacatcgcgg gcccgggttt
    54541 catcaatttc catctcacgg ccgcggcgcg ccaggccgtg ctggatgaca tcgccgagca
    54601 gggccccgat tacggcttcc tgccgggaaa cgaccagaaa gtactggtgg agttcgtctc
    54661 ggccaatccc acgggccccc tgcatgtggg gcatgcccgc caggcggccc tgggtgatgc
    54721 cctctgcgat ctgttctcgt cgcaggggta tgcggtcacg cgcgagttct actacaacga
    54781 cgccggcaac cagatcaaca atctggccat cagcgtgcaa gcgcgggccc gcggcatcgc
    54841 acccgatgcc gccgacttcc cggcggacgg ctacaagggc gattacatcc tcgacatcgc
    54901 caacgatttc ctggcgggcg ccacggtgca gggcgccgat cgcgaggcgg tgacggcctc
    54961 gcgcgacatc gacgacctgg acgccatccg ccgtttcgcc gtggcgtatc tgcgccgcga
    55021 gcaggatctg gatctccagg cctttggcct gcgcttcgac aattacttcc tggaaagctc
    55081 gctctacagc agcgggcgag tggaaaagac cgtgcaggcg ctggtcggcg ccggccacac
    55141 gtacgagaac gacggcgcgc tgtggttgcg caccaccgaa ctgggcaccg gcgacgacaa
    55201 agaccgcgtc atgcgcaagt ccgacggctc ctatacgtat ttcgtgccgg acgtggccta
    55261 ccacgtcaac aaatgggaac gcggctttca ccgcgccgtc aacgtgcagg gcagcgacca
    55321 ccacggaacg gtggcacgcg tccgggccgg cctgcaaggc ctggaccagg gcattcccaa
    55381 ggattacccg gcctacgtgc tgcacaagat ggtcaaggtg gtccgcgatg gcgcggaggt
    55441 caagatttca aaacgcgcgg gcagctacgt cacgctgcgc gacctgatcg actgggtggg
    55501 gcgggacgcc gtgcgctatt tcctcatcca gcgccgcgcc gattccgaat tcgtgttcga
    55561 catcgatctg gccttgtcca agagtgaaga caatccggtt tattacgtcc agtacgcgca
    55621 tgcgcgcatc tgttccatgc tggcccagtc cggcgtgtcg cacgatgcca tcgccgcggc
    55681 tggcaccgat gtcttgacgg cgccgtctga attcgcgctg ctgcgccgtc ttgcgggctt
    55741 tcccgacatg ctggcgcaag ccacccagga actggcgcct catcatgtcg ccttctggct
    55801 gcgcgattgc gccgccgact tccacgcctg gtatggggcc gagcgtgtcc tggtcgacga
    55861 ttcgcaactg cgcctggcgc ggttgcgcct ggcgcaggcc accagccggg tgctcgccgg
    55921 cgggctgcgg ctgctggggg tttccgcccc cgaaaagatg taaggcgcca ccatggctcg
    55981 caaaactcgc aaatccggca aatcgcgcgg catgagcggc acgatgatgg ggctggtcgc
    56041 cggtctcatg ctgggtgtgg gcgcggcggt ggccgtggcc tttttcgtgc tggaagttcc
    56101 catgccgttc gtcgacaagg ccagccgcgc cccggccacc accctgctgc cgggcgtgcg
    56161 caacgcgcca gaccccaaca tcgggctcta cgggccggac gggccggccg gcaccgtgcc
    56221 cgcggggcag ggtgtgacgg tcgtcacgcc actgcctggc accggctcgg cggccaatgg
    56281 ccaggcaagc tcgaacgcgc tggccaatca ggggacggca gccggccaga acgcgccggt
    56341 tgaacagaat accccccata aggccaaatc gccccgttct ccagacgccc tcgggtcgct
    56401 gatcgccagc ctgggcaaga cacccgtgcc gggcacccag gccgagccta aaacagctcc
    56461 ccatcgtgcc cgaaccgagc cggcgcaagg cacccagacc gtgtactacc tgcaggcggg
    56521 ggcgttccat tcaagcaagg acgcggacgc catgaaggcc cgcatcctca tgctgggtct
    56581 gtcggcgcag gttgaggacg cctcggtcaa tggcgccacc ctccaccgcg tgcgggtcgg
    56641 cccgttcaag ggtatcgacg ccatgaatcg cgcccgggcg aaactgggcg acgacaaaat
    56701 cgcctcatcg gtcgtgcgcc actgacggcg gcatccgccg tctccgggaa cttgccggtc
    56761 cttcaacagt ctgtcttgaa cgttgctctg atcaggaaat cagtcatgtc acccaaaagc
    56821 ctctttctcc gtttgttcgc agtcatcgcc attggagcgg gaaccctgtt cgcccccgcc
    56881 agccaggccg tcaccgccac ccagcccttg tacgccacgc tggatccggc ccagacgcct
    56941 gacacgccgc ccggcaagat cgaggtgctg gaattcttcg cctacacctg cccgcactgc
    57001 aacgccatcg agccgttgct cagaaagtgg gaaaaaaccc tgccgtccga tgtcgtggtg
    57061 cacccggtgc ctgtggcgtt caacgccagc atgaccgacc tgcagaaact gtactactcg
    57121 ctggtggcga tcggccgcac cgatctgcac gacaaggtgt tcgccgccat tcacgaagaa
    57181 aacaagccca tctacgacga aaaggccatt ctggactgga tcgccacgca gggtgtggac
    57241 cgcgccaagt tcaaggcggt gttcgattc
//
LOCUS       DASTNK010000013         6246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_8557, whole genome shotgun sequence.
ACCESSION   DASTNK010000013 DASTNK010000000
VERSION     DASTNK010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8557"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1960
                     /locus_tag="VFR20_01035"
     CDS             <1..1960
                     /locus_tag="VFR20_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEU0229130.1"
                     /translation="ANLASGGVTGGALVIVGEDYGEGSSIMQERTHAFAMKSQIWLLD
                     PKPNLESLVKAVEDGFLLSEASHTPVILQMRIRGCHVHGRFTAKDNQRPAMTLSQALD
                     NPVRDVDRIVLPPASFLHEQEKIRERWPAAVDFITRHQLNEFVDGDLDDIGLILQGGL
                     YNGVIRSLQLLGLADHFGNTRIPLYVMNVTYPVVDAEIDRFCHGKKAVLLLEEGQPNY
                     LEQALNTILRQRGSDTVLAGKDVLPMAGEYTTAVMRDGLRTFLDKHCPTALRNQEADA
                     GYVAAHSNATTAGASVDHVPTEGLPAGGAHDRQNTDMMVAQVARRLDDSAPPKADPLD
                     LKDVVPARPAGFCTGCPERPIFSALTLAQESLGERHIAGDIGCHLFSILPPFNLGATT
                     MGYGLGAASAAAFGVRSAKRPIAIMGDGGFWHNGLSSGVGNAVFNKYDGVILIIDNYY
                     TSATGGQDILSSRALNRRRDTGHPIEKAVRGVGVDWVRRVDRTYDVTRMRAVIEEALT
                     TQAPGPKVIIAQSECMLNRQRREKPAFRKAVGSGRRAVKERLGVDADVCNGDHACIRL
                     SGCPSLTVRDSGDPLKEDPVAYIDNSCAGCGNCGEVAVSAILCPSFYKARVIHNPGRW
                     DRFVQAVRHRVIHYLQQRRQARLDQIAI"
     gene            1972..3576
                     /locus_tag="VFR20_01040"
     CDS             1972..3576
                     /locus_tag="VFR20_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HEU0229131.1"
                     /translation="MTTFFHPGTPIKIAILAMGGQGGGVLADWIVDLAEHAGWWAQTT
                     SVPGVAQRTGATIYYVELLPEAQADAAGRAPVLAMMPTPGDVDLVLAAELMEGGRAIQ
                     RGLVTPARTTLIASSHRSYAVSEKSAPGNGIADPNRVLDAGRAAAKRFYCLDLQAIAD
                     QAGSVISASLFGAVAGSGALPFGREEFEATIMRAGVGVEASLRAFHMGHDSAAGAPAQ
                     PPAVPSAGGQPSASHPDIPDTAASPRMQRLLDTIRHDFPEPAHTLLLAGVRRAADFQD
                     IAYAREYLDRLRDILALDEQFGGTEMAWALTTAAARQIAVAMTYNDVIRVADLKTRGE
                     RTERVRQEVRAENGQIILTTEYLHPRLEEVCGTLPAALGRRIGQSRVVSRALGRIFHN
                     GRFVESGSLHGFLTLYCLAGMRRFRRSTLRHHDEMAGMAQWLAQVEDIVRDDYGLAVE
                     MVNCRRFVKGYSDTLARGTGKYTRLSGAAKRLLGQPDAATRLRTLREAALADAQGTEL
                     DRLLALDELGMMGSSTPRPQVRPRAA"
     gene            3689..4666
                     /locus_tag="VFR20_01045"
     CDS             3689..4666
                     /locus_tag="VFR20_01045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HEU0229132.1"
                     /translation="MATLKLVIEDAHAETDAIRSFTLRTADGAPLPPFTAGAHLKVHI
                     PGLDEPRNYSLVCLTPESALFTAPERYHLGVRLEDPSQGGSRYMHRVAIGDTLTVEPP
                     KNDFPVHDAQGDDPPPLLIAGGIGITPVASMAAQLSLQGRPFALHYYGRSRGQMAFVE
                     ALSAQYGPALHAHADDAPSTMLTLDALLDGASPATHLYICGPKGMIDAIIERAHARGW
                     PQSHIHVELFTTPVPQSGDRGFDVELRQSGLTFHVPPDKTILEVLAEAGVDPMYDCTR
                     GECGVCQVTVLEGLPDHRDYYLSDAEKKAGNVMQICVSRSFSDKLVLDL"
     gene            4714..6039
                     /locus_tag="VFR20_01050"
     CDS             4714..6039
                     /locus_tag="VFR20_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEU0229133.1"
                     /translation="MNHYTDNPAAVRALVRPTEVHKDLFIDDEIFDLEMEHLFANTWV
                     YVGHGSQVPNPGDFHSTTVGNQPVIMVRHTDKTVRVLYNRCPHKGVMVTGDTCGNTGK
                     FFRCPYHAWTFKTDGSLLSIPLKKGYDPEVLTQCEASQGMAAIENVRDHRGFVFCRLN
                     PEGQSFEDFFGESLSTLDNMVDRSPEGRLEVAGAPLRYMHDCNWKMLVDNQTDTCHPM
                     VAHESSAGTAVRVWEGAPPGTPKPMAVQQFAPFISPYEFFEGMGIRVWENGHGHTGVH
                     NSIHAAYSEIPDYLEAMVRAYGEARAHEILGDTRHNTIYFPNIMVKGPIQTLRIFKPL
                     AADKTLVESWAFRLVGAPDVLLERTLMYNRLINAPTSVVGHDDHEVYERSQHGLHARG
                     REWVNVARLYEPTEADRKNEVVNGTSEWQMRNQYQAWARYITTTMREAA"
     gene            6036..>6246
                     /locus_tag="VFR20_01055"
     CDS             6036..>6246
                     /locus_tag="VFR20_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEU0229134.1"
                     /translation="MNSSVQALPVGTDQDLMGFVHAEARLLDERRFEEWLDLFTEDGR
                     YWMPLTPGQTDPILQGSLMYEDKLLL"
BASE COUNT         1171 a         2147 c         1916 g         1012 t
ORIGIN      
        1 ggccaatctg gcttctggcg gcgtcacggg cggggcgctg gtcatcgtgg gcgaagacta
       61 tggcgaaggg tcctccatca tgcaggaacg cacgcacgcg ttcgccatga aatcccagat
      121 ctggctgctc gaccccaagc caaacctgga aagcctggtc aaggcggtcg aagacgggtt
      181 cctgctctcg gaggccagcc acacgcccgt catcctgcag atgcgcatac gcggctgcca
      241 cgtgcatggc cgtttcaccg cgaaagacaa tcagcgcccc gccatgacgc tgtcccaagc
      301 attggacaac ccggttcgcg acgtggaccg catcgtgttg ccacccgcct cgttcctgca
      361 cgagcaggaa aaaatccgtg aacgctggcc ggccgccgtc gatttcatca ccaggcacca
      421 gctcaatgaa ttcgtcgacg gcgacctgga cgacatcggc ctgatcctgc agggcggcct
      481 gtacaacggc gtcatccggt cgctgcaact gctggggctg gccgaccatt tcggcaacac
      541 gcgcattccc ctgtacgtga tgaacgtcac ctacccggtg gtcgacgccg aaattgaccg
      601 cttctgccac ggcaagaagg cggtgctgct gctcgaggaa ggccagccca attatctgga
      661 gcaagccctc aacaccatcc tgcgccagcg tggcagcgac accgtgctgg ccggcaagga
      721 tgtcctgccc atggcggggg aatacaccac ggcggtgatg cgtgacggat tgcggacgtt
      781 cctggacaag cactgcccca ccgcgttgag aaaccaggag gcggacgctg gatacgttgc
      841 cgcccactcc aacgcaacca ccgccggcgc atcagtcgat catgtgccga cggaaggtct
      901 gccagccggc ggcgcgcacg accggcaaaa cacggacatg atggtcgcgc aggtcgcccg
      961 ccgactcgac gattcagcgc ccccaaaggc cgaccccctc gacctgaaag acgtcgtccc
     1021 cgcgcgtccc gccgggttct gcacgggctg ccctgaacgg cccattttct cggccctcac
     1081 gctggcgcag gaatccctgg gcgagcgcca tatcgcgggc gacatcggtt gccacctgtt
     1141 ttcgatactg ccgccgttca atctgggcgc caccaccatg ggctacggcc tgggcgcggc
     1201 gagcgcggcg gcgttcggcg tcaggtcggc caagcgcccc atcgccatca tgggcgacgg
     1261 aggcttctgg cacaacggcc tgtcgtccgg cgtgggcaac gccgtcttca acaaatacga
     1321 cggcgtcatc ctcatcatcg acaactatta cacctcggcg acgggcggcc aggacatcct
     1381 gtcgtcccgc gcgctgaacc ggcgccgcga caccggccac cccattgaga aggcggtgcg
     1441 gggcgtgggg gtggactggg tgcgccgtgt cgaccgcacc tacgacgtca cgcgcatgcg
     1501 cgccgtcatc gaagaagccc tcaccactca ggcgccgggc cccaaggtca tcatcgcgca
     1561 atcggaatgc atgttgaatc gccagcgccg tgaaaagccc gcattccgca aggcggttgg
     1621 ctcgggccgg cgcgccgtga aagaacgcct gggtgtcgac gccgatgtct gcaacggcga
     1681 tcatgcctgc atccgcctgt cgggctgccc gtcgctcacg gtgcgcgaca gtggcgaccc
     1741 gctcaaggaa gatccggtgg cctacatcga caatagttgc gccggttgcg gcaactgcgg
     1801 ggaagtggcg gtctccgcca tactgtgccc gtcgttctac aaggcccggg tcatccacaa
     1861 tccgggtcgc tgggatcggt tcgtgcaggc tgtgcgccat cgcgtcattc attacctgca
     1921 gcaacgccgc caggcgcggc ttgatcagat cgccatatag aaggcccgac catgaccact
     1981 ttctttcatc cgggcacacc catcaagatc gccattctcg ccatgggtgg ccagggcggc
     2041 ggcgtgctgg ccgactggat cgtcgatctg gccgaacacg ccggctggtg ggcccagacc
     2101 acctctgtgc ccggtgtcgc gcaacgcacc ggtgccacca tctactatgt cgaactcttg
     2161 cccgaggcgc aagccgatgc cgcggggcgc gccccggtgc tcgcgatgat gcccacgccg
     2221 ggcgacgtcg atctggtgct ggcggccgaa ctcatggaag gcggccgcgc catccagcgc
     2281 ggcctggtga cgccagcgcg caccaccttg atcgcttcat cacatcgcag ctacgccgtc
     2341 agcgagaaat ccgcgccggg caacggcatc gccgacccga accgggtatt ggatgccggt
     2401 cgcgccgccg cgaagcgctt ctactgcctc gacctgcaag ccatcgccga ccaggcgggc
     2461 agcgtaatca gcgcgagcct tttcggtgcc gtcgcgggta gcggcgcatt gcctttcggc
     2521 cgcgaggaat tcgaggccac catcatgcgc gcgggggtgg gcgtggaagc cagcctgcgc
     2581 gctttccaca tgggccacga ctcggcggcg ggcgcccccg cccaaccgcc ggcggttcct
     2641 tccgccgggg gccagccatc cgcaagccat cccgatatcc ctgacaccgc ggccagcccc
     2701 cgcatgcagc gcctgctgga caccatccgg cacgacttcc cggagccggc ccacacgctg
     2761 ttgctggcgg gagtgcgccg cgctgccgat ttccaggata tcgcctacgc ccgggaatat
     2821 ctggaccgcc tgcgcgatat cctcgcgctg gatgagcaat tcggcggcac ggaaatggcc
     2881 tgggcgctga cgaccgccgc cgcgcgccag atcgccgtgg ccatgaccta caacgacgtc
     2941 atccgggtgg ccgacctgaa gacccggggc gaacgcaccg agcgcgtgcg ccaggaagtc
     3001 cgggcggaga acggccaaat catactgacg acggagtacc tgcatccacg cctcgaggaa
     3061 gtctgcggca ccctgccggc ggctttgggg cggcgcattg ggcagtcgcg cgtggtttcc
     3121 cgcgcgcttg gccggatctt ccacaatggc cgctttgttg aaagtgggtc gttgcatggt
     3181 tttctcacct tgtattgcct ggcggggatg cggcgcttcc ggcgcagcac gctcaggcat
     3241 cacgatgaaa tggccggcat ggcgcagtgg ctggcccagg tcgaggacat cgtgcgcgac
     3301 gactatggtc tcgccgtcga aatggtgaac tgccggcgtt tcgtcaaagg ctacagtgac
     3361 accctggcgc gtggcaccgg caagtacacg cggctgagcg gcgccgccaa gcggctactg
     3421 ggccagcccg acgccgccac gcgcctgcgc accctgcgcg aagcggcgct ggccgatgcc
     3481 cagggcaccg agctcgatcg gctgctggcg ttggacgagc tgggcatgat gggctcgagc
     3541 acgccacgtc cccaagtgcg gccgcgagcg gcctaagtcg agaactggaa atcctcgaca
     3601 tggcaggaac cccgtcctat ttacgaaaca aacccacgat gcacgacaca tccacgcttg
     3661 ccccggcatt gcacacagcg agacacacat ggcgactttg aaattggtca tcgaagacgc
     3721 gcacgcggag acggacgcga tccgatcgtt cacgttgaga accgccgacg gcgcgccgct
     3781 cccgccgttc acggccggcg cgcacctgaa ggtgcatatc cctgggctgg acgagccacg
     3841 caactactcc ctggtttgcc tgactccgga atccgccctg ttcaccgccc cagagcgcta
     3901 ccacctgggt gttcgactgg aagaccccag ccaaggcggc tcgcgttaca tgcaccgggt
     3961 agccattggc gacaccttga ccgtcgaacc tccaaagaac gactttccag tccacgacgc
     4021 ccagggcgac gaccccccac ccctgctcat cgcgggcggc ataggcatca cacccgtggc
     4081 gtccatggcc gcgcaacttt cactgcaagg ccggccattc gcattgcatt actacggccg
     4141 cagccgcggc caaatggcct tcgtcgaggc gctgtcggcg caatacgggc ccgcgctgca
     4201 tgcgcatgcc gacgacgccc cctcgacaat gctcacgctg gacgccctgc tggacggcgc
     4261 ctcaccggcc acgcacctgt acatctgcgg ccccaagggg atgatcgacg ccatcatcga
     4321 acgcgcccac gcgcggggat ggccgcagtc gcacatccat gtcgagctct tcacgacacc
     4381 cgtgccgcaa tcgggtgacc ggggattcga tgtggaactg cggcagtccg gcctgacctt
     4441 ccacgttccg cccgacaaga cgatactcga ggtattggcc gaagccggcg tcgacccgat
     4501 gtacgactgt acccgcggcg aatgcggggt atgccaggtg acggtcctgg aaggcctgcc
     4561 ggaccatcgt gactattacc tttccgacgc cgagaagaag gcgggcaacg tcatgcagat
     4621 atgtgtctcc cgcagctttt ccgacaaact ggtgcttgat ctttgaccca gggcctgttt
     4681 aaaaaaggag ccctccctgg aggaacccat gccatgaacc actataccga caatcccgcc
     4741 gccgtccggg cgctggtgcg ccccaccgag gtgcacaagg acctgttcat cgacgacgag
     4801 atcttcgatc tcgagatgga gcacctgttc gccaacacct gggtgtacgt gggccacggc
     4861 agccaggttc ccaaccccgg cgacttccac agcaccaccg tgggcaacca gcctgtgatc
     4921 atggtccggc acacggacaa gaccgtccgg gtgctctaca accgctgccc gcacaaaggc
     4981 gtgatggtca caggtgacac ctgcggcaac accggcaagt tcttccgctg cccgtatcac
     5041 gcctggacct tcaagaccga cggttccctg ctgtccattc cgctgaagaa aggctacgac
     5101 cctgaagtcc tgacgcagtg cgaggccagc caaggcatgg cggccatcga gaacgtgcgc
     5161 gaccatcgtg gcttcgtctt ctgccgcctg aaccccgaag gccagtcgtt cgaggatttc
     5221 ttcggcgaat cgctctccac cctggacaac atggtcgacc gctcgcccga gggccggctg
     5281 gaagtcgcgg gcgcgccgct gcgctacatg cacgactgca actggaaaat gctggtcgac
     5341 aaccagacgg acacctgcca ccccatggtg gcgcatgaat cctcggcagg cacggccgtg
     5401 cgcgtctggg aaggcgcccc ccctggcacg cccaaaccga tggcggtgca acagttcgct
     5461 ccgttcatct cgccctatga attcttcgaa ggtatgggga tacgcgtatg ggagaacggg
     5521 cacggccaca ccggcgtgca caattccatc cacgccgcgt attcagaaat accggactac
     5581 ctggaagcca tggtgcgggc ctatggcgaa gcgcgcgccc acgaaatcct gggcgacacc
     5641 cgccataaca ccatctactt tcccaacatc atggtcaagg gccccatcca gactctgcgc
     5701 atcttcaagc cgctggccgc cgacaagacg ctggtcgaat catgggcgtt ccgcctcgtg
     5761 ggcgctcccg acgtgctgct ggagcgcaca ctgatgtaca accggctcat caatgcgcca
     5821 acctcggtcg tggggcatga tgatcacgag gtttacgagc gatcacaaca tggcctgcac
     5881 gcgcgcgggc gcgagtgggt caatgtggcc aggctgtacg aacccaccga agcggaccgc
     5941 aagaacgaag tggtcaatgg caccagcgaa tggcagatgc gcaatcagta ccaggcctgg
     6001 gcccgctaca taaccaccac catgagggag gccgcatgaa ttcgtccgtt caagctttgc
     6061 cggtcggcac cgatcaggac ctgatgggtt tcgtgcacgc cgaagcgcgt ctgctggacg
     6121 aacgccgctt cgaagagtgg ctggacctgt tcaccgagga cggccggtac tggatgccgc
     6181 tgaccccggg gcagaccgac ccgatcctgc agggctcgct gatgtacgaa gacaagctcc
     6241 tgctgc
//
LOCUS       DASTNK010000014         4664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_380294, whole genome shotgun sequence.
ACCESSION   DASTNK010000014 DASTNK010000000
VERSION     DASTNK010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380294"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1660
                     /locus_tag="VFR20_01060"
     CDS             68..1660
                     /locus_tag="VFR20_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEU0229135.1"
                     /translation="MQRRRFLAHLARSSGIVLAAKASLALAGGMEGMSGMSGMRSMHG
                     MGGMHGMMKSAEGAAQVGSALAPSDAIPMGHPFAAPVALRNETPEPGKFKARLVAAPT
                     NLSLIPGINTQARTYNGQMPGPLIELRAGDEVEVTLENHLDQPTTVHWHGLPVPSDQD
                     GNPAEAVAPGGSRVYRFKLPDDVEGLYWYHPHPHRVSAEQVYRGLAGPILIRPRHDPL
                     AAIPERVLAVTDLRLTADGRIPVDDMMDWMNGREGQFALVNGMNRPILSFHSNGQERW
                     HLLNATNARYLRLTLPGHRFTLLGTDGGRIEQAQPDMREVLVAPAQRVELLVTAVGND
                     SAQLMAAPYDRGKMGGESATPAMALMHVDFSAVRQGVQPVVPKSLRKFPNLPPAVARK
                     RIVMSESMSMGAMSGGHGSSMKMDDPTMMEFLLNGKSFDMHRVDLRSKAGAWEDWEIV
                     NDSDMDHPFHVHGTQFAVLERVLDGRRHSEPFRAWYDTVNLKSRETVRIRIMQPQKGI
                     RMYHCHILEHEDLGMMGRLRVA"
     gene            1753..2706
                     /locus_tag="VFR20_01065"
     CDS             1753..2706
                     /locus_tag="VFR20_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEU0229136.1"
                     /translation="MAQIQIAALYKFASLPDYASLRAPLLACCGQNGVKGTLLLAEEG
                     INGTIAGPEQGVAAVLAFLREDPRLRSLEHKISWAPDMPFYRLKVKLKREIVTMGVAN
                     LSARAMAGTYVEPEQWNRLVDNPDVVVVDVRNDYEVSIGSFEGAVNPHTTSFAEFPQW
                     VREQSAKGGVLEDRPKVAMFCTGGIRCEKSTAYLRSQGFDEVYHLRGGILKYLETVPA
                     DASRWRGECFVFDERVSVTHGLAPGQYGLCRACRQPLGPAEMASPLYEEGVSCPHCHG
                     THTEDRAQRFRERQHQVELARARNQRHVGVRPGEPKDRSGQ"
     gene            complement(2695..3270)
                     /locus_tag="VFR20_01070"
     CDS             complement(2695..3270)
                     /locus_tag="VFR20_01070"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU0229137.1"
                     /translation="MKELTRCAWAGSDPQMRAYHDDEWGVPEYDSRALWEKLMLDGFQ
                     AGLSWSTILRKRQAFREAFQGFDPAVVARFGDTDVARLLANPGIVRSRSKIAATIGNA
                     RAYLAMRDAGEDFSGFVWGLAGGAPIVNTSPERPTQTPLSQAMSVALKKRGFKFVGPV
                     IVYAWMQATGIVDDHSPDCFRHRHCAPASLA"
     gene            complement(3308..>4664)
                     /locus_tag="VFR20_01075"
     CDS             complement(3308..>4664)
                     /locus_tag="VFR20_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU0229138.1"
                     /translation="SEVKASMSLGWPIILTNLAQLAILTTDVILIGRLGPEPLAAGSL
                     SISLYQIVMIFCMGLVSATIPILSTTLGRRRHAVGDVRRTVRQGLWMAALVSVPAWLM
                     LWHADSLLIALGQDPTLARQSLPLMHALQWALLPELGYIVLRSFLASIERPAWTLLVA
                     FGAIAFNAVAGWCLILGHGGFVPLGLRGAGIASAISSALMFTGLALIVVRHPRFRRYH
                     LFGHLAQPDWKRFIELWRLGLPIAVTFTLESLVFYTAVMLMGLIGDTALAAHAIAIQI
                     ASTTFMVPLGFGQVATIRVGLAAGRGDPLAAGRAGWTAYALGVGFMGIMALIMFLAPH
                     ALIGLFLDTSAPANQPVVDLAVRFLAVAALFQIADGAQTVSAGMLRGLRDTRIPMILA
                     AIGYWLLGVPTGAFLAFHTDLRGVGVWIGLATGLTVVAVLLTLRWVRYHGAMRLRVTS
                     A"
BASE COUNT          900 a         1436 c         1529 g          799 t
ORIGIN      
        1 ggcaaaaccg ggttcaccgt gtgatgcgga ggggcccgtt cgccacgcaa acaatggatg
       61 acagaccatg caacgtagac gcttcctcgc ccatctcgcc cgctcgtcgg ggatcgtcct
      121 cgcggcgaag gcctcgctgg cgcttgcggg cggcatggag ggcatgtccg ggatgagcgg
      181 catgcgatct atgcacggca tgggcggcat gcatggcatg atgaaatccg cggaaggggc
      241 ggcgcaggtg ggctcggcgc ttgccccatc cgacgccatc cccatggggc atcccttcgc
      301 ggcgcccgtc gcgctgcgca atgaaacccc ggagcctggg aagttcaagg ccagactggt
      361 cgccgcgcca accaatctat ccctcatccc cggtatcaac acacaggctc ggacctacaa
      421 cgggcagatg ccgggaccgt tgatcgaact gcgtgcgggc gatgaggttg aagtcacctt
      481 ggaaaatcac ctcgatcagc cgacgaccgt tcattggcat ggcctgcccg tcccgtccga
      541 tcaagatggc aatcctgcgg aggctgttgc gccgggcggc agccgggtgt atcggttcaa
      601 gcttcccgac gacgtcgagg gcctgtattg gtaccatcct catccgcatc gagtgtccgc
      661 cgaacaggtt taccgagggc tcgcgggccc catcctgatc cgccccaggc atgacccgct
      721 ggccgccatc cccgagcgcg tcctagccgt caccgatcta cgcctgacgg cggacggtcg
      781 gattccggtt gacgacatga tggactggat gaatggccgc gagggtcagt tcgcgttggt
      841 caacggcatg aatcgcccga tactgtcgtt tcactccaat ggacaggaac gctggcacct
      901 gctcaacgcc accaatgccc gttatctgcg gttgacgctg ccgggacacc gtttcacgct
      961 gttgggtacc gacggcgggc gtatcgagca ggctcaaccc gatatgcggg aggtcctggt
     1021 cgcaccggcg cagcgtgtgg aactgttggt gacggcggtc ggcaacgata gcgcccaact
     1081 catggccgcg ccctatgatc gcgggaaaat ggggggcgag agcgcgacgc ccgccatggc
     1141 gctgatgcat gtggatttca gtgcggtgcg acaaggtgtg caaccagtcg ttccgaagtc
     1201 attgcgcaaa tttccgaatc tgccccccgc cgttgcgcgt aagcggatcg tcatgagcga
     1261 atcgatgtcc atgggcgcca tgtcgggtgg acacggttcg tccatgaaga tggatgatcc
     1321 gacaatgatg gaattcctgc tgaacggcaa atcgttcgac atgcatcgcg tggacctgcg
     1381 cagcaaggcg ggtgcgtggg aagactggga gatcgtcaac gattccgaca tggatcatcc
     1441 tttccacgtg catggcacgc agttcgccgt gctggagcgt gtcctcgatg gccgcaggca
     1501 cagcgaaccg ttccgcgcgt ggtacgacac tgtgaatctg aaatcccgcg aaaccgtgcg
     1561 gatccgcatc atgcagccgc agaaagggat tcgcatgtac cactgccaca tcctggagca
     1621 cgaagatctc ggaatgatgg ggcgcctccg ggtggcgtga cgaaccgttg ccttaaaatc
     1681 ccgagcgtgg tgtcgctcat ctttggtgtt tgccgccgct gatccgttgt tttcctctcg
     1741 ctccattcac ccatggccca gatccagatc gccgcgctgt acaagttcgc ctcactaccc
     1801 gattacgcgt cactgcgcgc gcctctactg gcgtgctgcg ggcagaatgg cgtcaagggc
     1861 acgctgctgt tggccgagga gggaatcaat ggcacgattg ccggcccgga gcagggggta
     1921 gcggcggttc tggcgttcct gcgtgaggat ccccgcctgc gatccctgga gcacaagata
     1981 tcctgggcac cggacatgcc gttctatcgc ctgaaggtca agctcaagcg ggagatcgtc
     2041 accatgggtg ttgcgaacct gtcggcgcgc gcgatggcgg gaacctatgt cgagcctgag
     2101 cagtggaata ggctcgtcga caacccagat gtggtggtgg tcgacgtgcg caacgactat
     2161 gaggtgtcca tcggctcgtt cgagggcgcg gtgaacccgc acaccaccag cttcgcggag
     2221 tttccccagt gggtgcgtga acagtcggcc aaaggaggtg tgctcgaaga caggccaaag
     2281 gtggcgatgt tctgcacagg tggaatccgc tgcgagaaat ccacggccta cctgcgctct
     2341 caggggttcg acgaggtcta ccacttgcgc ggcggcatct tgaagtatct ggaaaccgtg
     2401 ccggccgacg caagccgttg gcggggggag tgctttgtct tcgatgagcg ggtgtcggtg
     2461 acgcatgggc tggcgccggg ccaatatggg ttgtgccgcg cctgtcggca gccactgggg
     2521 cctgcggaaa tggcgtcgcc actctatgaa gagggcgtca gctgcccgca ttgccacggc
     2581 acccatacgg aagatagagc gcagcggttt cgcgaacgcc aacatcaagt ggagctggcc
     2641 agggcaagga accagcgcca tgtaggcgtg cgaccgggtg aaccgaagga tcgctcaggc
     2701 caatgaggcc ggggcgcaat gcctgtgccg aaagcagtcg gggctgtggt cgtcgacgat
     2761 tcctgtggct tgcatccacg cgtacacgat gaccggaccg acgaacttga agccgcgctt
     2821 cttcagcgcg accgacatgg cttgtgacaa gggcgtttga gtcggccgtt caggactggt
     2881 gttgacgatt ggggcgcctc ccgccaaccc ccagacaaac cccgaaaagt cttcaccggc
     2941 atcccgcatg gccaggtagg cacgcgcatt gccgatggtg gctgctattt tcgagcgcga
     3001 gcgcacgatg ccaggattgg cgagcaggcg cgccacgtcg gtgtcgccga accgtgcgac
     3061 gacggccggg tcgaacccct ggaacgcctc gcggaatgcc tggcgtttgc gcaggatggt
     3121 ggaccatgac agccccgcct ggaaaccgtc cagcatgagt ttttcccaaa gagcacggct
     3181 atcgtattcc ggaacacccc attcgtcgtc gtggtacgcc cgcatctgtg ggtcggagcc
     3241 ggcccaggcg catcgtgtca attccttcat ggttgtcgtc tcctggttta tttcggaaat
     3301 acgcgattca ggcgcttgtg acccgcaagc gcatcgctcc gtgatatcgc acccatcgca
     3361 gggtcagcag caccgccacg acggtcaggc ccgtggccaa ccctatccac acgcccacgc
     3421 cgcgcaaatc ggtgtggaac gccaggaagg cacccgtggg cacacccaga agccagtagc
     3481 cgatggcggc cagaatcatg ggaatgcgcg tgtcgcgcag accgcgcagc attcccgcgc
     3541 tgacggtttg cgccccgtcg gcgatctgga acaaagcggc cacggcgagg aaacgcaccg
     3601 ccagatcgac cacgggctgg ttggcaggcg cgctcgtgtc caggaacagg ccgatgaggg
     3661 cgtgtggggc gaggaacatg atcagcgcca tgatacccat gaaccccacg cccaaagcat
     3721 aagcggtcca ccccgcgcgg cccgctgcga gcgggtctcc acgccccgcc gccaggccga
     3781 cgcgtatggt ggcgacctgg ccgaaaccaa gcggcaccat gaaggtcgtc gacgcgatct
     3841 gtatggcgat ggcgtgcgcc gccaacgcgg tgtcgccgat caggcccatc agcatgacgg
     3901 cggtgtagaa caccagtgat tcgagggtga acgtcacggc gatgggaagg cccaggcgcc
     3961 atagctcgat gaatcgcttc cagtcgggtt gggcaaggtg cccgaacaga tggtagcggc
     4021 ggaagcgtgg atgcctgacg acgatgagcg ccaggccggt gaacatcagc gcgctggaga
     4081 tggcgctcgc gatccccgcg ccgcgcagtc cgagcggcac gaatccgcca tggcccagga
     4141 tcaggcacca accggccacc gcgttgaacg ctatggcgcc gaacgcgacc agcagcgtcc
     4201 aggcgggccg ctctatggaa gccaggaacg agcgcaggac gatataaccc agctcaggga
     4261 gcagagccca ttgcagcgcg tgcatgagtg gcagggactg ccgcgccaga gtggggtcct
     4321 ggcccagggc gatgagcagg gagtcggcgt gccatagcat gagccaggcc ggcacactga
     4381 ccagggctgc catccagagc ccctggcgca cggtgcggcg cacgtcgcca accgcgtgtc
     4441 ggcgacgtcc caatgtggtc gacaatatgg gaatggtggc cgagaccagg cccatgcaga
     4501 aaatcatcac gatctggtac agactgatcg acagcgaccc cgccgccagg ggctccggcc
     4561 ccaaccgccc gatcagtatg acgtcggtcg tgagtatggc cagttgcgcc aggttcgtca
     4621 ggatgatggg ccagcccagg gacatcgacg ccttgacttc cgat
//
LOCUS       DASTNK010000015        19957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_9442, whole genome shotgun sequence.
ACCESSION   DASTNK010000015 DASTNK010000000
VERSION     DASTNK010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 19957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19957
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9442"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFR20_01080"
     CDS             <1..207
                     /locus_tag="VFR20_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU0229139.1"
                     /translation="WLARERKLAIRQDDLLAILSAGAYAFAMASQYNTRARAAEVMVD
                     GDDYRVVRPRENLESLFAGETLWA"
     gene            424..2238
                     /locus_tag="VFR20_01085"
     CDS             424..2238
                     /locus_tag="VFR20_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU0229140.1"
                     /translation="MNLPPKPSDSWNDCAVCSTSESVLHSQRGGIPIFAITAWLWTGL
                     VLFYVAILLPRQYFTPMFWLASVLGLATCVTWLAQGLRTGFQAKTPLWYALALTIALV
                     SGLLHQSLPAEDIPVLHNNWPCMVTLLAASILASLAIAEHRRRLFVEASHGSPAATRG
                     PRDVHGWLEASLDQVMVSHGFCLAYLDLNPLRVINDVYGYTTGDDIFRQVVQRIEPML
                     SDHHHLGRVGGEAIVIVFQHTPLDEARLIAQRILDDVRHTPCHIGPRRFRPQGFMGLV
                     EITTPLRAQDALSVAVRACRMAGQSHPGTVGAYQHDAIEIDEHFQVLALLKNMGNGLL
                     PERLFLDMQPILSLQDPAGSLNFEVLLRMRNTHGGDIPTTRLINIAEENGMMPFIDKW
                     VFHTTLEWLQRHHRRLNHTQFVCINLSGMSLNDEAFIDDFFITLDNYQHLATLLCVEI
                     TEGVALSNKESTRHVIRRIQAQGVRVALDDFGAGYTSFSYLAELPANIIKIDGQFIRS
                     MKDNAASVAIIQAIIALAHNLGMKSIAEWVEDVDTMETLRDLGVDYVQGNAITPPMRP
                     GELLSIDDITSHIVDKDVRSTVLAGAKSGARHGTRALH"
     gene            complement(2272..3261)
                     /locus_tag="VFR20_01090"
     CDS             complement(2272..3261)
                     /locus_tag="VFR20_01090"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU0229141.1"
                     /translation="MSFMLADSLETPGQAVNSLAGMVLSTQLELLNSFLPMACWLVAL
                     LGRDQVEIVSSIGEQVALADRLISCLGEWPGSGSDTGLVRRRVKSAELATLVATGYAG
                     RMPTHAFRAPLRGVRGQLSGMVLGCSCEEADVLRATGPAREIELCLRAISRTLNLYTA
                     LTATEKLVYEARQRAQIDPLTKVLNRAGWNRQLRNLAATGVEVAIGLLDLDYLKFTND
                     TRGHQAGDALLQSTGRAIKSVLRANDSVARLGGDEFAVLLFDVTPDVMHDLRERLQRA
                     LADTDVAASIGMALRSESGSLANAMQLADIRMYQEKRGKQRIRMASVDACDFE"
     gene            complement(3757..5118)
                     /gene="ccsB"
                     /locus_tag="VFR20_01095"
     CDS             complement(3757..5118)
                     /gene="ccsB"
                     /locus_tag="VFR20_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEU0229142.1"
                     /translation="MSDTLTNPRPTPMWDKAMSASGDSRGGRGKPDWTDILFFLVLAS
                     GAAYAMRNYGADMNYYSKLILGGAVVVFSWLAWMWRPIRYLIIACGLTALFAVWLYSD
                     GTGLSHGNLSRAESVFFLKYFFSSQTAILWMCSLFVLATVCYWIGLFSNTAAWLGTVF
                     AWGAVYFGLTGLLVRWRESYLMGPGIGHIPVSNLYEVFVLFSLITSLFYLYYERRYAT
                     RALGGFVLMVVSSAVVFLLWYSFTRDAYQIEPLVPALKSWWMKLHVPANFIGYGTFSL
                     AAMTGFAYLVKEHGQTVSRAKLAPLFVLGAILCAEPFVFSTRAVSPVWMLYFGVGAII
                     VAAILALRRPIAAKLPALEVLDDIMYRSIAVGFAFFTVATILGALWADNAWGTYWQWD
                     PKETWALIVWLNYAAWLHMRLMKGLRGSMAAYWALGGLLVTSFAFLGVNMFLSGLHSY
                     GSL"
     gene            complement(5131..7188)
                     /locus_tag="VFR20_01100"
     CDS             complement(5131..7188)
                     /locus_tag="VFR20_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEU0229143.1"
                     /translation="MNMDTVKTLSLSRRGADFVELLGSMRFAVSLLVFVCVASLIGTV
                     LTQNMDASAYIDKFGPFWFSVFDKFSIWHIYNSWWFLVIMTFLVVSTTICLIRNTPKM
                     LRDARSFREYIRASSLRAFHHRVECESGAGAAENLSHIQEWLRQQGYRYKLRQDGDST
                     MVAAKKGSANRLGYIFTHAAIVIVLLGGLLDSQMPIRVQIWLGLRKPIEQNMLISQVP
                     ASGRLSAGNLSFRANMLIPEGGESSNGLVSIDNGVLVQPLPFIVKLKRFYVSYYSTGM
                     PSNFKSDVEIIDPTDGKKFQQTIEVNEPLRYKGLTIYQSSFDDGGSKLKLVGYPLAGS
                     GDTPFDVNGTVGRSTEITMGSGSNAPRMDVDLSGLRTINVEDMSNGKPPQPKPFLKEV
                     AAVTGSAAGAKNKNLHNVGPSVQYRITGSDGQSHEFNNYMLPMTLDGIPVFLAGVRES
                     SNENYRYLRIPADADHSVAEFMRLRAALNDPVMRAEAAARFAAKNATAKIQEPLLDKA
                     AQGALETFSKAGFNGIVERAPKQDREKIMSFAVPMIQLTLTELRDLERAKAGLPPVNY
                     TGPSGQQAEKWIQTALLALANLPQYPAPVFMSLKSFDHIEASVFQVARSPGTAIVMLG
                     CLLLVVGVFSMFFIRERRIWVWIKPQGSGSELRAAMTSQRRNIDFQRDFEQFKDAVER
                     LST"
     gene            complement(7275..7973)
                     /locus_tag="VFR20_01105"
     CDS             complement(7275..7973)
                     /locus_tag="VFR20_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU0229144.1"
                     /translation="MKQVFSKVLLASGLLLGVSMVSASFATEVAVPKPDAAKGEQLFT
                     KGDPARGIVACVTCHGAGGNSTIASNPNLAAQPHEYLAEQLESFRSKDGKAPLRRGKG
                     GADTVMSAFAKALTKSDIQNVAYYLAQQKLDWAKAGVATDKTTVALGQRIWRGGIPDR
                     RVPACASCHSANGAGLPGKFPRLAGQHPGYLVEQLTLFRNGDRANSDMMHEIANRLTD
                     ADIAAVADYAAGLR"
     gene            8075..8698
                     /gene="yihA"
                     /locus_tag="VFR20_01110"
     CDS             8075..8698
                     /gene="yihA"
                     /locus_tag="VFR20_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEU0229145.1"
                     /translation="MSILHRAAFSISAARLDQLPAPTTAEVCFAGRSNAGKSSAINVL
                     TNQRRLAFSSKTPGRTRLINLFGIPDPLEPGASLGFLVDLPGYGYAATGKETRGQWAD
                     MLGGYLRERTSLAGIVLLVDIRRGVTDLDRRLADWIAPTGKPVLVLLTKADKLPYGQR
                     RIAEFAARKQLADIGALHVVPFSATQRIGLEDATLQIENWISPKVVP"
     gene            8695..9711
                     /gene="hemB"
                     /locus_tag="VFR20_01115"
     CDS             8695..9711
                     /gene="hemB"
                     /locus_tag="VFR20_01115"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEU0229146.1"
                     /translation="MTSFLPSSDFPSLRLRRNRRDDFTRRLVRENTLTPADFIYPVFV
                     REGKGVHEPVPSMPDVVRYSPDTVLEAAEDCVRLGIPVLALFPVIEPSLKTPDGIEAV
                     NPEGLIPRTVAVLKSRFPELGVLTDVALDPYTSHGQDGVIDHDGYVLNEPTVEILTQQ
                     ALAQARAGVDIVAPSDMMDGRIGMIRRALEENRHIHTRIMAYSAKYASAFYGPFRDAV
                     ASASNLGKSNKAAYQMDPANRNEALREVAADIREGADMVMVKPGMPYLDILRDVKNAF
                     GMPTFSYQVSGEYAMLKAAAANGWLDHDKVMMESLIAFKRAGGDGILTYFAIAAATLL
                     KEQA"
     gene            complement(10504..12675)
                     /gene="dsbD"
                     /locus_tag="VFR20_01120"
     CDS             complement(10504..12675)
                     /gene="dsbD"
                     /locus_tag="VFR20_01120"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEU0229147.1"
                     /translation="MMSFLVRARPGRLPLRFGAKGAIARSLAAGHAWRIRVVGVMALL
                     LLCLFAATYANAEQKFLEPEQAFSFSATMASPTEIDIRYKIAPGYYMYRGRFELALSP
                     DAQRLGTPAYPKGIVKYDPNFDKEVEIFHDQVLIKVPLKPGAIAPQTLDVTGQGCAEA
                     GLCYPPMTTALTLHPTATGYTVSGPGAQAGVAGGAGVVNGTSTGNVSGFDAALHMGDT
                     GIAKFLAGAGWGEIILLCVVFGVLLSFTPCVLPMVPILLAIVAGGANARATVSRWRGL
                     SLAAAYVLGMSLVYTALGIAAGLIGASLSAWLQTPWVLTLFAVVLALLALAMFDVFTF
                     QAPGAFQSALSEKVSRIPGGRAGAAFLMGMISALIVGPCVAAPLAGVLLFISQTGNVV
                     LGGVALFSLAWGQGVLLLLVGGSSGALLPKAGGWMDGVKHLFGVLLFATAWWMVNSVV
                     PDWLGALGWAVLGAWCAVMLGAFEPMGSEASQGAVLRKAAGLLLALWSALIVVGLAVG
                     GHDVWQPLRVLRSTVGVSSVTVAPSSTTLSPVAAGSPVGKATPPAPFASSASTASPAA
                     SVSSATPAASATPAASGALASALTGGFSLAKPVATTGRFTRVASSAELDALLARTTRP
                     VMLDFYANWCVSCIEMEKFTFSDPQVAARMSKFLLVQADVTKNNADDRALLKRFQLFG
                     PPGIMFFDAHGKLIADARVVGFQDATKFSAELDRVLAAAGK"
     gene            complement(12672..13058)
                     /gene="cutA"
                     /locus_tag="VFR20_01125"
     CDS             complement(12672..13058)
                     /gene="cutA"
                     /locus_tag="VFR20_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEU0229148.1"
                     /translation="MGATTDAASGLEAGRDVVVVLSNAPDPLLAKRIAHVLVEESLVA
                     CATLGSPSLSMYMWNGVLEGADEIPMMFKTTRARVPDVLARLRQLHPDEIPEALVLPV
                     AGGLNSYLDWVVERTSAPPLHEENNQ"
     gene            complement(13275..13661)
                     /gene="rplQ"
                     /locus_tag="VFR20_01130"
     CDS             complement(13275..13661)
                     /gene="rplQ"
                     /locus_tag="VFR20_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU0229149.1"
                     /translation="MRHRSGLRKLNRTSSHRLAMFRNMAVSLITHEAIKTTLPKAKEL
                     RRVVEPLITLGKEPTLANKRLAFARLRDRDAVLKLFAEIGPRYKERNGGYTRVLKMGF
                     RQGDAAPMAFMELVDRPEVAEADDAE"
     gene            complement(13914..14900)
                     /gene="rpoA"
                     /locus_tag="VFR20_01135"
     CDS             complement(13914..14900)
                     /gene="rpoA"
                     /locus_tag="VFR20_01135"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU0229150.1"
                     /translation="MSSQGFLKPRSIDVVPVGRNHAKVVMEPFERGYGHTLGNALRRI
                     LLSSMTGYAPTEVQMTGVVHEYSTVAGVREDVVDILLNLKGVVFKLHNREEVTLVLRK
                     QGQGSVLASDIELPHDVEIINPDHVIATLTEAGKLEMQIKVEQGRGYVPGNVRALADD
                     RTHTIGRIVLDASYSPVRRVSYAVESARVEQRTDLDKLVLDIETNGVVSPEEAVRQSA
                     RILMDQISVFAALEGAGDSYEAPAHGAPQIDPVLLRPVDDLELTVRSANCLKAENIYY
                     IGDLIQRTENELLKTPNLGRKSLNEIKEVLAARGLTLGMKLDNWPPLGLERP"
     gene            complement(15055..15678)
                     /gene="rpsD"
                     /locus_tag="VFR20_01140"
     CDS             complement(15055..15678)
                     /gene="rpsD"
                     /locus_tag="VFR20_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU0229151.1"
                     /translation="MARYIGPKCKLSRREGTDLFLKSAQRSLDSKCKLESKPGQHGRT
                     SGARLSDYGVQLREKQKLKRMYGVLEKQFRKYFAEAERRRGNTGETLIQLLESRLDNV
                     VYRMGFGSTRAEARQLVNHRAIELNGHTADIASMLVKSGDVITVREKAKAQTRIRASL
                     DLAAGNGLPQWVEVDASKLSGTFKQAPDRADVARDVNESLVVELYSR"
     gene            complement(15690..16091)
                     /gene="rpsK"
                     /locus_tag="VFR20_01145"
     CDS             complement(15690..16091)
                     /gene="rpsK"
                     /locus_tag="VFR20_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU0229152.1"
                     /translation="MAKASNSGATRVRKKVKKSVSDGIAHVHASFNNTIITITDRQGN
                     ALSWATSGGAGFKGSRKSTPFAAQVAAESAGRVALEYGIKTLEVRIKGPGPGRESSVR
                     ALNALGIKISSISDITPVPHNGCRPPKRRRI"
     gene            complement(16107..16472)
                     /gene="rpsM"
                     /locus_tag="VFR20_01150"
     CDS             complement(16107..16472)
                     /gene="rpsM"
                     /locus_tag="VFR20_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU0229153.1"
                     /translation="MARIAGINIPPHQHAEIGLTAIFGIGRTRARKICEASGVDVSKK
                     VKDLTDAELERIREHVGVFTVEGDLRREVQLSIKRLIDLGTYRGMRHKRGLPVRGQRT
                     RTNARTRKGPRRAAASLKK"
     gene            complement(16508..16621)
                     /gene="rpmJ"
                     /locus_tag="VFR20_01155"
     CDS             complement(16508..16621)
                     /gene="rpmJ"
                     /locus_tag="VFR20_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU0229154.1"
                     /translation="MKVMASVKRICRNCKIIKRHGVVRVICTDPRHKQRQG"
     gene            complement(16654..16872)
                     /gene="infA"
                     /locus_tag="VFR20_01160"
     CDS             complement(16654..16872)
                     /gene="infA"
                     /locus_tag="VFR20_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU0229155.1"
                     /translation="MAKDDVIQMQGEVVENLPNATFRVKLENGHVVLGHISGKMRMHY
                     IRILPGDKVTVELTPYDLSRARIVFRSK"
     gene            complement(16882..18207)
                     /gene="secY"
                     /locus_tag="VFR20_01165"
     CDS             complement(16882..18207)
                     /gene="secY"
                     /locus_tag="VFR20_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU0229156.1"
                     /translation="MAKAQAQGKSGSRYGDLKRRVVFLVLALVVYRLGTHVPVPGINP
                     DALSDLFRQNQNGILGLFNMFSGGALERFSVFALGIMPYISASIIMQLMAVVVPSLEA
                     IKKEGQSGQRKITQYTRYGTVFLALIQSAGIAVALQSQPGLVIDPGMMFQFTTIVTLV
                     TGTMYLMWIGEQITERGLGNGISILIFAGIVAGLPSALGGMLDLVRTDSMSVLSALFV
                     VVLVVAVTYFVVYVERGQRRITVNYAKRQVGNKVYGGQASHLPLKLNMSGVIPPIFAS
                     SIILLPATVTSWFSNSPHMRWLGDLAAAITPGQPLYITLYSALIIFFCFFYTALVFNS
                     RETADNLKKSGAFVPGIRPGEQTSRYIDKILMRLTLAGAIYITLVCLLPELLQARWNV
                     PFYFGGTSLLIIVVVTMDFMAQAQAYLMSQQYDSLLKKANFKGAGLPMR"
     gene            complement(18219..18659)
                     /gene="rplO"
                     /locus_tag="VFR20_01170"
     CDS             complement(18219..18659)
                     /gene="rplO"
                     /locus_tag="VFR20_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU0229157.1"
                     /translation="MSETQLNTLRPAEGSKHAKRRVGRGIGSGLGKTAGRGHKGQKSR
                     TGGFHKVGFEGGQMPLQRRLPKRGFTPLGQKLYAEVRLSDLERLPVDEIDVQVLKQAG
                     IIGQAVRYAKVIKSGELSRKVALKGVSATAGARAAIEAAGGSLA"
     gene            complement(18670..18855)
                     /gene="rpmD"
                     /locus_tag="VFR20_01175"
     CDS             complement(18670..18855)
                     /gene="rpmD"
                     /locus_tag="VFR20_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU0229158.1"
                     /translation="MSQKQIKVTLVRSVIGTMKSHRATVRGLGLTRVNSSRVLVDTPE
                     VRGMIRKVDYLVTVSEA"
     gene            complement(18859..19380)
                     /gene="rpsE"
                     /locus_tag="VFR20_01180"
     CDS             complement(18859..19380)
                     /gene="rpsE"
                     /locus_tag="VFR20_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015551161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU0229159.1"
                     /translation="MAKAQGKHAVEQERDDGMREKMIAVNRVSKVVKGGRTMSFAALT
                     VVGDGDGRIGMGKGKAREVPVAVQKAMEQARHGLIKVALKNGTLHHTVVGKHGASTVL
                     ISPAAEGTGVIAGGPMRAIFDVMGVRNVVAKSLGSSNPYNMVRATLNGLRACSTPAEV
                     AAKRGKTIEEILG"
     gene            complement(19394..19759)
                     /gene="rplR"
                     /locus_tag="VFR20_01185"
     CDS             complement(19394..19759)
                     /gene="rplR"
                     /locus_tag="VFR20_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU0229160.1"
                     /translation="MDKKTSRLRRATATRRKINELRVHRLSVFRSNLHIYANIISPEG
                     DRVLVSASTLEPEVRKELAGQKANGGNAAAAGLVGKRVAEKAKAAGIEQVAFDRSGFR
                     YHGRVKALADAAREAGLKF"
     gene            complement(19781..>19957)
                     /gene="rplF"
                     /locus_tag="VFR20_01190"
     CDS             complement(19781..>19957)
                     /gene="rplF"
                     /locus_tag="VFR20_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU0229161.1"
                     /translation="GIKIECPTQTEIIVRGSDKQAVGQVAAEIRAYRRPEPYKGKGVR
                     YADERIVLKETKKK"
BASE COUNT         3853 a         6372 c         6068 g         3664 t
ORIGIN      
        1 tggctcgcgc gcgaacgcaa actggccatc cggcaggatg atctgctggc catcctgtcc
       61 gccggagcct acgctttcgc catggcaagc caatacaaca cacgcgcccg cgccgccgaa
      121 gtcatggtcg atggcgacga ctaccgcgtc gtgcgccccc gggaaaacct ggaaagcctg
      181 ttcgctggcg aaaccctctg ggcctgacga cggggcggca tccgggctcg gccacgccat
      241 ttttcattcc atcgaattga gggcaaaaac ccgggtaaac gtctcgattg acctgccgat
      301 tattcgacta gattgggcca gcggtgctgg gcacagagca tgtgtcggca cgtgccactt
      361 ccaagccatt cgaaaaccat caaggatcct taagaattgg ccaggaatgc cgttttccga
      421 ctaatgaatc ttccccccaa gccctccgat tcctggaatg attgcgcggt ctgctccacg
      481 agcgagtccg tcctccactc ccagcggggc ggcattccga tattcgcgat cacggcctgg
      541 ctatggaccg gactggtcct gttctacgtt gcgatcctcc tccctcgtca gtacttcact
      601 ccaatgttct ggctcgccag cgtactcggg ctagccacct gcgtaacctg gctcgcgcag
      661 gggctgcgca ccggattcca agcgaagacc ccactttggt atgcgttggc actaacgatt
      721 gcactggtgt ccggcctgct ccaccagtcg ttgcccgcgg aagacatccc agttctccac
      781 aacaactggc cgtgcatggt cacgctgctt gccgccagca tcctcgcatc gctggccatc
      841 gccgaacacc ggcgacgcct gttcgtggaa gccagccacg gcagtccggc cgcgacgcgg
      901 gggccacgcg atgtccatgg atggctcgag gcgtcccttg atcaagtgat ggtcagccac
      961 ggcttctgcc tggcgtacct tgacttgaac ccgctgcgcg tcatcaacga cgtatacggc
     1021 tatacgaccg gagacgatat tttccgccaa gtggttcagc gcatcgagcc catgctttca
     1081 gaccaccatc atctcggtcg cgttggcggc gaggcgatcg tcatcgtgtt ccagcacacg
     1141 ccgctggacg aggcgcggct catcgcgcag cgcatactcg acgacgtccg tcacacaccc
     1201 tgccacatcg gccctcggcg gttccggccc caggggttca tgggtctggt cgagatcacc
     1261 actccgctgc gcgcccagga tgccctgtcg gtcgccgtgc gagcctgccg catggcgggg
     1321 cagtcccatc caggcaccgt cggcgcctac cagcacgacg ccatcgagat cgacgagcac
     1381 ttccaggtgc tggcgctgct caagaatatg ggcaatggct tgctgccgga acggctgttt
     1441 ctcgacatgc aacccattct ctcgctgcag gacccggccg gctcactgaa ttttgaagtg
     1501 ctcctgcgca tgcgcaacac gcatggcggc gacattccca ccaccaggtt gatcaacata
     1561 gctgaagaaa acgggatgat gccattcatc gacaaatggg tgttccacac cacactcgaa
     1621 tggctgcagc ggcatcaccg ccgactgaat cacacccagt tcgtctgcat caacctgagc
     1681 ggcatgtcgt tgaacgacga agccttcatc gacgacttct tcattacact cgacaactac
     1741 caacacctgg cgacactact gtgtgtggaa atcaccgagg gggtggcgct gagcaacaaa
     1801 gaaagcacgc gccacgtcat ccggcgaata caggcccagg gggtgcgtgt cgcactggat
     1861 gatttcggtg ccggctacac gtcattttct tacctggctg aactcccggc gaacatcatc
     1921 aaaatcgacg ggcagttcat ccggagcatg aaagacaatg ccgccagcgt cgccatcatc
     1981 caggccatta tcgccctggc ccacaatcta ggcatgaaga gcatcgccga atgggtggaa
     2041 gacgtcgaca ccatggagac gctgcgcgat ctgggtgtgg actatgtgca aggcaacgcg
     2101 atcaccccac ccatgcggcc cggcgagttg ctgtcgatcg acgacatcac cagccatatc
     2161 gtcgacaagg atgtccgctc gacagtactc gcgggcgcga aatcgggcgc gcgccatgga
     2221 acccgtgcgt tgcactgacg gaatacagca ccggagcgcg tcgtcgtctt tctattcgaa
     2281 gtcgcaggcg tcgacgctcg ccatcctgat gcgctgctta ccacgctttt cctgatacat
     2341 gcgaatgtcc gccaactgca tggcattggc cagcgagccg gattctgagc gcaacgccat
     2401 gcctatggac gcggcgacat cggtatcggc cagcgccctc tgcagccgtt cacgtagatc
     2461 atgcatgacg tccggcgtga cgtcgaacag caacaccgca aattcgtcac ccccaaggcg
     2521 cgccacacta tcgttcgcgc gcaggaccga cttgatggcc cgacccgtgg actgcagcaa
     2581 ggcatcaccc gcctggtggc cgcgcgtgtc attagtgaat ttgaggtagt ccaggtcgag
     2641 cagaccgatg gcgacttcga cgccggtggc ggccaggttg cgcaattgcc tgttccaacc
     2701 ggcgcgattg agcaccttcg taaggggatc gatttgtgca cgctgtcgtg cctcgtagac
     2761 cagtttctcg gtggctgtga gcgccgtgta cagattcagc gtgcgcgata tggcgcgcag
     2821 acagagttcg atttcgcgcg ccgggccggt tgcgcgaagc acatcggcct cctcgcagga
     2881 acatccgagc accatgccgg acaactgccc ccgcacgccg cgcaacggtg cccggaacgc
     2941 atgggtaggc atccgccccg cataacccgt ggccacgagc gtggcgagct cggctgactt
     3001 gacgcgacgc cggacgagcc cagtgtcgga cccactcccg ggccactcac ccagacaact
     3061 gatgagccgg tcggcaaggg ccacttgttc gccaatcgag gatacgatct cgacctggtc
     3121 gcggcccagc agggcgacca accagcaagc catgggcagg aaggagttca gcagctcgag
     3181 ctgagtggac aacaccatcc cggcaaggga attgaccgct tgcccaggtg tttcaaggct
     3241 gtcagcgagc atgaacgaca tctggcgacc cctggcggtg ttggtcgaca cgcttggcga
     3301 aaaggcccgc acaaaacacg aaccccctca acccctgatg gccggggcat gattggtttc
     3361 aataggcacc tgtgacctgt aaccaatatg tcctggggtc gtaattatat attgcgatta
     3421 gattaaaagt aagaccagat tcgccggcca cagcgcatga tgctgatcgc aaagtgcagg
     3481 gtcctgcgat ttctcacatt tactttccag caacacaccg caaaacagcc tgaacaggca
     3541 gcatatggcg ggccagccac catatgcttc aaaagactac gaaagacaaa ggtgtggcag
     3601 cgtctcacca ggcacgaatg cgtccatatg gctcacgggc cgccaatccg gccaccacgc
     3661 tgatgacgtc gagaggaaaa gaaccaggcc tgtccaccat gatggcctgt gcggcgttcc
     3721 gattggaacc cgcacaggaa ggcgaacggt tgaaccctac aggctaccgt acgaatgcag
     3781 gcctgacaga aacatgttga cacccaggaa ggcaaagctg gtgaccagca gcccgcccag
     3841 cgcccagtat gccgccatgc tgccgcgcag ccccttcatg agccgcatgt gcagccacgc
     3901 cgcgtagttc agccagacga tgagcgccca ggtttccttg ggatcccatt gccagtacgt
     3961 gccccaggca ttgtcggccc acagcgcgcc caggatggtg gcgaccgtga agaacgcaaa
     4021 ccccaccgcg atggagcggt acatgatgtc gtccagcacc tcgagcgcgg gcagcttcgc
     4081 ggcgatgggc cggcgcaacg ccagaatcgc cgccacgatg atggcgccca cgccgaaata
     4141 cagcatccag acaggcgaga ccgcgcgggt gctgaagacg aaaggttcgg cgcacaggat
     4201 ggcgcccagt acgaacagcg gcgccagctt ggcgcgcgac acggtctgcc cgtgctcttt
     4261 gaccagatag gcgaacccgg tcatggcggc cagcgagaac gtgccatagc caatgaaatt
     4321 ggccggcaca tgcagcttca tccaccagct tttgagcgcg ggcaccagcg gttcgatctg
     4381 gtaggcatca cgcgtgaacg aataccacag caggaacacg accgcggaac tgaccaccat
     4441 cagcacgaac ccgcccagcg cccgcgtcgc gtagcggcgt tcgtaataaa gatagaacag
     4501 tgacgtgatc agcgagaaca gcacgaagac ttcatacaga ttgctgactg ggatgtggcc
     4561 gatgccgggc cccatcaggt aactttcgcg ccagcgcacc agcaggccgg tcaggccgaa
     4621 gtacaccgca ccccaggcga acaccgtgcc gagccacgcc gccgtgttgc tgaacagacc
     4681 gatccagtag cacacggtgg ccagcacgaa caacgagcac atccagagaa tggccgtctg
     4741 tgacgagaag aagtacttga ggaagaagac gctttccgcc cgcgacaggt tgccgtggct
     4801 gagccccgtg ccatcgctgt acagccagac cgcgaacaac gccgtcagcc cgcaggcgat
     4861 gatgaggtag cggatgggcc gccacatcca ggcaagccaa ctgaacacca ccaccgcgcc
     4921 acccagaatg agcttgctgt agtagttcat gtcggcgccg taattacgca tcgcgtacgc
     4981 cgcgcccgat gccagcacca ggaaaaagag gatgtcggtc cagtcgggct tgccgcggcc
     5041 gccacgcgaa tctccgctgg cggacatcgc cttgtcccac atgggcgtcg gcctcggatt
     5101 ggtcagagta tcggacatga atggacctcg ttacgtggat aggcgttcga cggcgtcttt
     5161 gaattgttcg aaatcgcgct ggaaatcgat gttccggcgc tgcgacgtca tggccgcccg
     5221 taattcgcta cccgagccct ggggcttgat ccagacccag atccggcgtt cgcgaatgaa
     5281 aaacatagag aacacgccca ccaccagcag caggcatccc agcatcacga tggcggtgcc
     5341 cggactgcgc gccacctgga agacgctggc ctcgatatgg tcgaaactct tcaacgacat
     5401 gaacaccggc gcgggatact gcggcaggtt ggccagtgcg agcaacgccg tctggatcca
     5461 tttttcagcc tgctggcccg acgggcccgt gtagttgacc ggcggcagac cggccttggc
     5521 acgctccaga tcgcgcaatt cggtcagggt gagttgaatc atgggcacgg cgaaactcat
     5581 gatcttttca cgatcctgct tcggcgcacg ttcgacgatg ccattaaacc ccgctttgga
     5641 gaacgtttca agcgcgccct gcgctgcctt gtcgagcagc ggctcctgta tcttggcggt
     5701 cgcgttcttg gccgcgaacc gggcggcggc ctcggcgcgc atgaccgggt cgttcaacgc
     5761 ggcgcgcagc cgcatgaact cggccacgga atgatccgca tcggccggaa tgcgcaggta
     5821 gcggtaattt tcgttggacg actcgcgcac gccggccagg aacaccggaa tgccgtcgag
     5881 cgtcatgggc aacatgtaat tgttgaattc gtgcgactga ccatcgctgc ccgttatgcg
     5941 gtattgcacg ctgggaccga cgttgtgcag attcttgttc ttcgcgcccg ccgcgctgcc
     6001 ggtgacggcg gccacttcct tcaggaacgg cttgggttgc ggtggcttgc cgttggacat
     6061 gtcctcgacg ttgatggtgc gcagccccga cagatcaaca tccatgcgcg gggcgttgga
     6121 accgcttccc atggtgatct cggtggaccg gcccacggtg ccgttgacat cgaacggggt
     6181 atcgccggac ccggccagcg ggtagcccac cagcttgagc ttgctgccac cgtcatcgaa
     6241 actggactgg tagatcgtca gccccttgta gcgcaacggc tcattgactt cgatcgtctg
     6301 ctggaatttc ttgccgtcgg tcggatcgat gatctcgacg tcgctcttga aattactggg
     6361 catgcccgtc gagtaatagc tgacatagaa ccgcttgagc ttgacgatga acggcaaggg
     6421 ctgcaccagc acaccgttgt cgatgctgac caggccgttg ctgctttcgc cgccctcggg
     6481 gatgagcatg ttggcgcgga agctgagatt ccccgcggaa agccggccgg aagcgggaac
     6541 ctgtgagatc agcatgttct gttcgatggg cttcctgagc cccagccata tctggacccg
     6601 gatcggcatc tggctgtcga gcaggccgcc cagcagcacg atgacgatag cggcgtgcgt
     6661 gaagatgtag cccagacgat tggcgctgcc cttcttcgcg gcgaccatcg tgctgtcgcc
     6721 atcctggcgc agcttgtatc ggtagccctg ctgacgcagc cattcctgta tgtgcgacag
     6781 gttttccgcc gcgcccgcgc cggactcgca ctcgacgcga tgatggaacg cgcgcaggct
     6841 gctggcccgg atgtattcgc ggaacgaacg cgcgtcacgc agcatcttgg gtgtgttgcg
     6901 gatcaggcag atggtggtgg acacgacgag gaacgtcatg atcaccagga accaccagga
     6961 attgtagata tgccagatcg agaacttgtc gaacaccgaa aaccagaacg gcccgaactt
     7021 gtcgatgtaa gcgctggcgt ccatgttctg tgtcagcacg gtgccgatca ggctggccac
     7081 gcacacgaac accagcagac tgacggcgaa ccgcatcgag cccagcagtt cgacgaagtc
     7141 ggcgccacgg cgtgaaagag acaaagtctt cactgtatcc atattcataa aaaaaggggt
     7201 gccgtcagca acggcccccc ccgatgttct gctgcgccgc gcgggagaag ccccgcgccc
     7261 tgtggtaact ggctttaccg caaacccgcc gcgtaatcgg ccacggccgc gatgtccgcg
     7321 tcggtgaggc ggttggcgat ttcgtgcatc atgtcgctgt tggcgcggtc accgttgcgg
     7381 aacagggtga gctgttcaac cagataaccg gggtgctgtc ctgcaaggcg ggggaatttg
     7441 cccggcaggc cggcaccgtt ggccgaatgg caactggcgc aggccggcac acgacgatcg
     7501 ggaatgccgc cgcgccagat gcgctggccc agcgccacag tggttttgtc ggtggccacg
     7561 ccggccttgg cccagtcgag tttctgctga gccaggtaat acgcgacgtt ctggatgtcg
     7621 gacttggtaa gcgccttggc aaaggcgctc atgaccgtgt cggcgccacc cttgccgcga
     7681 cgcaacggcg ctttgccgtc tttggaacgg aaggactcca gttgttcggc caggtattcg
     7741 tggggttgcg cagccaggtt ggggttggac gcaatggtgc tgttgccgcc cgcgccatgg
     7801 caggtcacac aggccacgat gccgcgcgcc gggtcaccct tggtgaagag ctgttctccc
     7861 ttggctgcat cgggtttcgg gacggccacc tcggtggcga aactggcgga aaccattgaa
     7921 acacccagca gcaaaccgct ggccagcaac actttggaaa acacttgctt catgaagacc
     7981 tcgacgaccg cgattcgatc aggaagatga acccaactgt ttcaaacgca caattataca
     8041 atagcgtacc tatccaaccg ccccaagcca ccctgtgtct attctgcatc gcgccgcttt
     8101 ctctatttcc gccgcccgcc tcgaccagct tcccgccccc acaacggctg aagtgtgctt
     8161 cgcgggccgc tccaacgcgg gtaaatcgtc ggccatcaat gtcctgacca accagcgtcg
     8221 gctggcgttt tccagcaaga cccccgggcg cacacgcctg atcaatctgt tcggcatccc
     8281 cgacccgctg gagcctggcg catcgctggg tttcctcgtc gatctgccgg gttacgggta
     8341 tgcggcgacg ggcaaggaaa cgcgcggcca gtgggccgac atgctgggcg gctacctgcg
     8401 cgaacgcacg tcgcttgccg gcatcgtctt gctggtcgac atccgccgcg gcgtcaccga
     8461 cctcgatcgt cgcctggccg actggatcgc gcccaccggc aagcctgtgc tggttctgct
     8521 gaccaaggcc gacaagctgc catacgggca gcgccgcatc gccgaattcg ccgcgcgcaa
     8581 acagcttgcc gacatcggcg ccctgcatgt cgtgccgttc tccgccaccc agcgcatcgg
     8641 cctggaagac gcaacgctgc aaatcgaaaa ctggatttcc ccaaaggtcg taccatgaca
     8701 tcttttctgc cttcctccga ttttccttcc ctgcgcctgc ggcgcaaccg ccgcgacgac
     8761 ttcacccgcc gcctcgtgcg cgaaaacacg ctcacgcccg ccgacttcat ctaccccgtc
     8821 ttcgtgcgcg aaggcaaagg ggtgcatgaa cctgtgcctt ccatgcccga tgtcgtgcgc
     8881 tattcgcctg acaccgtcct cgaggccgcc gaagactgcg ttcgcctggg catcccggtg
     8941 ctcgcgctgt tcccggtcat cgaacccagc ctgaaaaccc ccgatggcat cgaggccgtg
     9001 aaccctgaag ggttgattcc ccgcaccgtg gccgtgctga aatcgcgctt ccccgaacta
     9061 ggcgtcctga ccgacgtggc gctcgacccc tacaccagcc acggccagga tggtgtcatc
     9121 gaccacgacg gctacgtgct gaacgaaccc acggtcgaga tcctcaccca gcaggcgctg
     9181 gcgcaggcgc gcgccggcgt ggacatcgtg gcgcccagcg acatgatgga tggacgtatc
     9241 ggcatgatac gtcgggccct ggaagagaac cgccatatcc acacgcgcat catggcgtac
     9301 tcggcgaagt acgccagcgc gttctacggg ccgttccgcg acgcggtggc gtcggcgtcc
     9361 aatctgggca agtcgaacaa ggcggcctat cagatggacc cggccaatcg caacgaagcc
     9421 ttgcgcgaag tcgcggctga tatccgcgaa ggcgccgaca tggtgatggt caagccgggc
     9481 atgccctatc tggatatcct gcgcgacgtg aagaacgcct tcggcatgcc cacgttctcg
     9541 taccaggtca gcggggagta cgcgatgctc aaggctgcgg cggccaacgg ctggcttgat
     9601 cacgacaagg tgatgatgga gtcgttgatc gccttcaagc gggctggcgg cgacggtatt
     9661 cttacttact tcgcgattgc ggctgccacg ttgctgaaag aacaggcctg agtccgcgag
     9721 gtgggtgctt gcggggctct gtaggtatct gaagacgctg tctctcggag tcaggggcgt
     9781 gcccggcacg gcgtgcttca tccgaatctg tctcgtccag gcgggcgtcg ggcgaaactc
     9841 gcaaaacggt cagtcctgcg gtctaaccgt tttgctcaaa cagtcgcccg acgactgccc
     9901 ccgcctggcc aagccagatc cggcgcacgc cttacaccgg gcccacccct ggccccgata
     9961 gccagctccc gttgatggcg tgcaggggag tcggcaagcc tgtttcgggc aaagagcatg
    10021 acgtcgtgga tatcttgaat taccgcacag ctctcaaatc gtcgtgtggg ccttgatgcg
    10081 tccaatcgtc cataacctcg gcggtatcct ccatgcatgc cgctgtcgta gccccaggat
    10141 gggggaagtg gtgcaggccg gcgtttcggc cagcgccgga tctggcttgg ccaggcgggg
    10201 gcagtcgtcg ggcggtgtct gaacggagcc tcgcgatagc gaggcgtagt gagttcagcc
    10261 cgacgcccgc ctggacgaga cagatccggg cagttggggc gcgcagcgcc acgaccgctg
    10321 gacgcgccgg cctgcaccac ctcccccacc ctggggcggg ttaattaata gaatgatcac
    10381 cccgcccagg accgttcaaa ccaccaaaac ggtcaaaaca acccaaacaa ctcgaatgac
    10441 cgaaacggtt caacccgtag cgttatcggt ggtcaacacg gattcatatc gcccgaaaat
    10501 gtcctatttc cccgcagccg ctagtacgcg atcgagttcg gccgagaatt tggttgcgtc
    10561 ttgaaagccc accacgcgcg cgtcggcgat caatttgcca tgcgcgtcga agaacatgat
    10621 tccgggtggg ccgaacagtt ggaagcgttt gagcagcgcg cgatcgtcgg cgttgttctt
    10681 ggtgacgtcg gcctgcacca gcaggaactt cgacattcgg gccgccactt gcggatcgct
    10741 gaaggtgaac ttctccatct ctatgcacga cacgcaccag ttcgcgtaga aatccagcat
    10801 gactggacgt gtggtgcgcg ccagcagagc gtccagttcg gccgacgagg ccacccgggt
    10861 gaatcgtcca gtcgtcgcga ccggcttcgc caacgaaaac ccgcccgtca gcgcactcgc
    10921 caacgcgccc gaggcggctg gtgtggccga agcggctggt gtggctgacg atactgatgc
    10981 ggctggcgat gccgtcgatg cggacgaggc aaaaggagcg ggaggcgttg ccttcccgac
    11041 tggagatccc gccgcgacag gcgacagtgt cgtggaggat ggcgccaccg tgacggacga
    11101 taccccgacg gtggatcgca atacccgcaa aggctgccag acgtcatgtc cgccaaccgc
    11161 cagccccacg acaatcaacg ccgaccacaa cgccagcagc aaccctgcgg ccttgcgcag
    11221 cacggctcct tggctggcct ccgatcccat gggctcgaag gcgcccagca tcacggcgca
    11281 ccatgccccc agcaccgccc agcccagcgc gcctaaccaa tcgggcacga ccgagttgac
    11341 catccaccag gcggtggcga acagcaagac cccgaacaaa tgcttgacgc catccatcca
    11401 gccccccgcc ttggggagca acgcgcccga cgacccaccg accagcagca gcaacacccc
    11461 ctgcccccag gccagcgaga acagcgccac accccccagg accacgttgc cggtctggga
    11521 aatgaacagc aacacgcccg ccaatggcgc ggccacgcac ggcccgacga tcagcgccga
    11581 gatcatgccc atgaggaacg ccgctcccgc gcgcccgccg gggatgcggg agaccttttc
    11641 actgagtgcc gactggaagg caccgggcgc ctggaacgtg aacacatcga acatggccag
    11701 cgccagcagt gccagcacca ccgcgaacag cgtcagcacc cagggtgtct gtagccacgc
    11761 cgacaggctg gcgccgatca gcccggccgc gatgccgagt gcggtgtaca cgagcgacat
    11821 gcccagcaca taggccgcgg ccaacgacaa accgcgccag cgcgataccg tcgcgcgcgc
    11881 gttcgcgcca ccggccacga tcgccagcag gatgggcacc atgggcaaca cgcacggcgt
    11941 gaacgacagc agcactccga acacgacaca cagcaggatg atctcgcccc agcccgctcc
    12001 agccaggaac ttggcaatgc cggtgtcgcc catgtgcagg gccgcgtcaa aacccgagac
    12061 attgccggtc gacgtgccat tgaccacgcc ggcccccccc gccacaccgg cctgcgcgcc
    12121 ggggccgctg accgtgtacc cagtggctgt gggatgcaat gtcagggccg tcgtcatggg
    12181 cggatagcac aggccggctt cggcgcaccc ctgtcccgtg acatccagcg tttgcggcgc
    12241 gatcgccccc ggcttcaggg gtaccttgat cagcacctgg tcatggaaga tttcgacttc
    12301 tttgtcgaaa tttggatcgt acttgacgat acccttcgga taggccggcg tgcccagccg
    12361 ctgcgcgtcg ggagaaagcg caagctcgaa acgccctcgg tacatgtaat agcccggcgc
    12421 gatcttgtag cggatgtcga tctcggtggg cgatgccatc gtggccgaga aggaaaacgc
    12481 ctgttcgggt tcgagaaact tctgttcggc attcgcgtat gtggccgcga acaggcacag
    12541 cagcagcaac gccatcacgc ccacgacacg gatccgccac gcatggcccg ccgccagcga
    12601 tcgggcaatg gcgccctttg cgccgaaacg cagcgggagc cgtcccgggc gggctcgaac
    12661 cagaaacgac atcattgatt gttctcttca tgcaggggcg gtgccgaggt gcgttccacc
    12721 acccaatcca ggtaactgtt cagcccgccc gccaccggca acaccagcgc ctcgggaatc
    12781 tcgtccggat gcagctgccg caaacgcgcc agcacatctg gcacgcgtgc gcgagtcgtc
    12841 ttgaacatca tggggatttc gtccgcgcct tcgagcacgc cattccacat gtacatagac
    12901 agagagggcg aacccagcgt ggcacacgcc accagggatt cttcaaccag cacatgcgca
    12961 atgcgcttgg ccaacagcgg atcgggcgcg ttgctcaaca ccacgaccac gtcccgccct
    13021 gcctcaaggc ccgatgccgc gtcagttgtc gcgcccagcc gggttggagt gtcgtcacgc
    13081 cgcgcaccag agggcggctg gtcggtacct gccatgaagc ttccaaaaaa agacggattg
    13141 ccgcgaacca ttgtagcgaa tcaagcgcgt agaagatcgt ctcgatccag aagtgacaag
    13201 ccccgcggcg ctacgcgctc gcggggcttg tggcgccacg gccaccgtcg acggcggcca
    13261 cgactattcc ggcgttactc ggcgtcgtcg gcttcggcga cttccgggcg atcgaccagt
    13321 tccatgaaag ccatgggggc ggcgtcgccc tgacggaagc ccattttcag gacacgggtg
    13381 tagccgccgt tgcgctcttt gtagcgcggg ccgatttcgg cgaacagctt gagcaccgcg
    13441 tcgcggtcgc gcaggcgggc gaacgccagg cgcttgttgg ccagcgtggg ttctttgccc
    13501 agggtgatca agggctcgac gacacggcgc aattccttgg ccttcgggag cgtggtcttg
    13561 atggcttcgt gggtgatgag cgagacggcc atgttgcgga acatggcaag acggtgactg
    13621 ctggtgcgat tgagtttacg caagccgcta cggtgacgca tgatgagttc ctttgaactt
    13681 tgaatctaag gggcggaatg aattccgcga tgaaccggct cttctatcag tcagactgcg
    13741 ggccgggtgg gtggcggttc atggcatcgt cgccattgcc cgcctgatgt accgtttgac
    13801 ctggccgggc atccaagtgc tggatacccg gataaatcta acggggcact gattttaacc
    13861 tgccgcgcgc cgcgccgttg cttcgtccga agcgccggcg cggcatcgcg cgatcagggg
    13921 cgttccaggc ccagcggggg ccagttgtcg agcttcatgc ccaaggtaag gccgcgcgcg
    13981 gccaggactt ccttgatctc gttgagcgac ttgcgaccca ggttcggggt cttgagcaat
    14041 tcgttctcgg tgcgctggat caggtcgccg atgtagtaga tgttttcggc cttcaggcag
    14101 ttggccgacc gcacggtgag ttccagatcg tccaccgggc gcagcaggac cgggtcgatc
    14161 tgtggcgcgc catgcgcggg cgcttcgtac gaatcacccg cgccttccag cgcggcgaag
    14221 acggagatct gatccatcag aatgcgggcc gactggcgca cagcttcttc gggcgacacg
    14281 acaccgttgg tctcgatgtc gagcaccagt ttgtcgaggt cggtgcgctg ttcgacacgg
    14341 gcgctttcca cggcgtagct gacgcggcgc acggggctgt aggacgcgtc gagcacgata
    14401 cggccgatgg tgtgggtacg atcatcagcc agggcgcgca cgttgccggg cacatagcca
    14461 cggccctgct cgaccttgat ctgcatttcc agcttgccgg cttcggtcag cgtggcgatg
    14521 acgtgatcgg gattgatgat ttccacatcg tgcggcagct cgatgtcgct cgccagcaca
    14581 ctgccctggc cttgcttgcg cagcaccagc gtgacctctt cgcggttgtg cagcttgaac
    14641 accacaccct tgaggttcag caggatgtcg acgacatctt cacgaacgcc ggcgaccgtc
    14701 gagtattcgt gcaccacgcc agtcatctgg acttcggtgg gggcgtagcc cgtcatggac
    14761 gacagcagga tgcggcgcag cgcgttgccc agcgtatggc cgtagccgcg ttcgaagggc
    14821 tccatcacga ccttggcgtg attcctgcca acgggcacga catcgatgga gcgcggcttc
    14881 agaaaacctt gtgacgacat atgtgttcct tttcaatacc ctcggctcgt tacaccgata
    14941 aggctgacga acaaaccggc ttggccggcg aatggcatcg cggcaaggcc cacttcccgg
    15001 caatgccgga aagtgggcct gtcgcgacgt aagaagcgga tatccgcgcc ttgtttagcg
    15061 ggaatacagt tcgacgacga gggactcgtt cacgtcgcgc gcgacgtcgg cgcgatccgg
    15121 cgcctgcttg aacgtgcccg acagcttgct ggcgtcgact tcgacccact gcggcagacc
    15181 gttgccggcg gccaggtcca gcgaggcgcg gatgcgcgtc tgggccttgg ctttctcacg
    15241 caccgtgatc acgtcgccgg acttgaccag catggacgcg atgtcggcgg tgtggccgtt
    15301 gagttcgatg gcgcggtggt tcaccagctg gcgcgcctcg gcgcgcgtgg agccgaagcc
    15361 catgcggtac accacgttgt ccaggcgcga ctcgagcaac tggatcaggg tttcgccggt
    15421 gttgccgcga cgacgctcgg cttcggcgaa gtacttgcgg aactgctttt ccagaacgcc
    15481 gtacatgcgc ttgagcttct gcttttcgcg cagctgcacg ccgtagtccg acaggcgggc
    15541 acccgaggtg cggccatgct ggcccggctt cgattcgagc ttgcacttcg aatcgagcga
    15601 acgctgcgcg ctcttcagga aaagatcggt gccctcgcga cgcgaaagct tgcatttggg
    15661 tccaatataa cgtgccaagg tgcttcccct taaatgcggc ggcgcttggg aggacggcag
    15721 ccgttgtgcg gaaccggcgt gatgtccgag atgctggaga tcttgatgcc cagcgcgttc
    15781 agtgcccgca cggaggactc gcggccgggg ccgggtccct tgatacggac ttcgagcgtc
    15841 ttgatgccgt attccagcgc gacgcggccg gccgattcgg cagccacctg ggcggcgaac
    15901 ggcgtggact tgcgcgaacc cttgaagccg gcaccacccg acgtcgccca cgacagggcg
    15961 ttgccctgac ggtcggtgat cgtgatgatc gtgttgttga aggacgcgtg aacgtgcgca
    16021 atgccgtccg agacgctctt cttgactttc ttgcgaacgc gcgtagcgcc gctattggaa
    16081 gctttcgcca tcttgtgttc ctcggtttat ttcttcaggg acgcggcggc acggcgcgga
    16141 cccttgcggg tgcgggcgtt cgtgcgggtg cgctgaccgc gaacaggcag gccacgcttg
    16201 tgacgcatgc cacgataggt tcccaggtcg atcagacgct tgatcgagag ctgcacttcg
    16261 cgacgcaggt caccttcgac ggtgaagaca cccacgtgct cgcggatgcg ttcgagttcg
    16321 gcgtcggtca ggtctttcac cttcttcgag acatcgacac ccgaagcttc gcaaatcttg
    16381 cgagcacgcg tgcggccgat cccgaaaata gcggtgagac cgatttctgc atgttgatgc
    16441 ggcggaatgt tgatgccggc gatacgggcc atgactgttc cttgttaaat ccgttgcgtt
    16501 cgggccatca gccctgacgc tgcttgtgac gcgggtcggt acagatgaca cgcaccacgc
    16561 cgtgacgttt gatgatcttg caattgcggc agatccgctt taccgatgcc attaccttca
    16621 tggttgactc ctagtttcct gtaacccgcc gccctacttg gagcggaaaa caatcctggc
    16681 acgcgagagg tcatagggcg tgagctccac ggtgaccttg tctcccggca ggatccggat
    16741 gtaatgcatg cgcatcttgc cggagatatg gcccagcacc acatggccgt tttccagctt
    16801 gacgcggaac gttgcattgg ggaggttttc caccacctcg ccctgcattt gtatgacgtc
    16861 gtcttttgcc atctttcctt cttaccgcat tggcagcccg gcgcccttga aattggcttt
    16921 cttgagcagc gagtcgtatt gttgggacat caggtaggcc tgggcctggg ccatgaagtc
    16981 catggtaacc accacgatga tcaacagcga ggtgccgcca aagtagaacg gaacgttcca
    17041 gcgagcctgc agcaattcgg gcagcagaca caccagcgtg atatagatcg caccggccag
    17101 cgtcaggcgc atcaggatct tgtcgatgta gcgcgaggtt tgttcgccag gacggatacc
    17161 gggcacgaac gcgccgcttt tcttcaggtt gtccgcggtt tcccggctgt tgaacaccag
    17221 agccgtgtag aaaaagcaga agaaaatgat gagggccgaa tacaacgtga tgtacagggg
    17281 ctgccccggg gtgatggcgg ccgccagatc gcccagccag cgcatgtggg ggctgttgga
    17341 gaaccagctg gtcaccgtcg cgggaagcag aatgatcgac gacgcgaaga tgggcgggat
    17401 caccccggac atgttcagct tgaggggcaa atgtgatgcc tggccgccgt agaccttgtt
    17461 cccgacctgg cgcttggcgt agttgaccgt gatgcggcgc tgcccacgtt cgacatagac
    17521 cacgaagtac gtcacggcga ccaccagcac aaccacgaac agcgccgaca gcaccgacat
    17581 ggagtccgtg cggaccaggt cgagcatgcc gcccagcgcg ctgggcaggc cggccacgat
    17641 acccgcgaag atcaggatcg atatgccgtt acccagaccg cgttcggtga tctgctcgcc
    17701 gatccacatc aggtacatcg tgccggtcac cagcgtgacg atcgtggtga actggaacat
    17761 catgcccggg tcgatgacca ggccaggctg cgattgcagc gccaccgcga taccggctga
    17821 ctggatcagc gccaggaaca ccgtgccgta gcgcgtgtac tgcgtgatct tgcgctggcc
    17881 ggactggcct tccttcttga tggcctcgag cgacggcacc accaccgcca tcaactgcat
    17941 gatgatggac gcggaaatgt acggcatgat ccccagcgcg aaaacggaga accgttccag
    18001 cgcgccaccg gagaacatgt tgaacaggcc caggatgccg ttctgattct ggcggaacag
    18061 gtcggacagg gcatcagggt tgatgcccgg cacgggaaca tgcgttccga ggcgatagac
    18121 gaccagagcc agcaccagga ataccacccg gcgcttcagg tcgccataac gcgaacccga
    18181 cttaccctga gcctgagctt tagccactcg ttaccccttc aagccagcga gccgccggcg
    18241 gcttcgatgg ccgcacgggc acccgcggtg gccgacacac ccttcagggc caccttgcgg
    18301 gacagttcgc ccgacttgat aaccttggca tagcgcacgg cctggccgat gatcccggcc
    18361 tgcttgagca cctggacgtc gatttcgtcg acaggcaggc gttcgagatc ggacaagcga
    18421 acttccgcat acagcttctg gcccagaggc gtgaagccgc gcttcggcag gcggcgttgc
    18481 aggggcatct gaccgccctc gaaaccaacc ttgtggaagc cacccgtgcg ggatttttga
    18541 cctttatggc cgcgaccggc ggtcttgccc aggcccgagc cgatgccgcg cccaacccgg
    18601 cgcttggcat gcttgctgcc ttcggcgggc cgcagcgtat tcaattgcgt ttctgacatc
    18661 tcgattcctt caggcttcgg agacggtgac gagataatcc accttgcgga tcatcccgcg
    18721 cacctctggc gtatcgacca ggacacgact gctgttgacg cgcgtgaggc caagcccgcg
    18781 caccgtggcg cgatgggact tcatggtgcc gatcacggag cgcacgagag tgactttgat
    18841 ttgtttctgc gacatgattt accccagaat ctcttcgatg gtcttgccac gcttggcggc
    18901 gacttccgcc ggcgtcgagc aggcgcgcag gccattgagg gttgcgcgga ccatgttgta
    18961 cggattgctg gaacccaggc tcttggccac gacgttacgc acgcccatga catcgaagat
    19021 cgcgcgcatc ggaccgccgg cgatgacgcc ggtaccttcg gcggcgggcg agatcagcac
    19081 ggtggacgcg ccatgcttgc caacgacggt gtggtgcagc gtaccgttct tcagggccac
    19141 cttgatcagg ccgtgacggg cctgttccat ggctttctgg acggcgaccg gcacttcacg
    19201 cgccttgccc ttgcccatgc cgatgcggcc gtcgccgtcg ccgaccacgg tcaacgcggc
    19261 aaagctcatg gtgcgaccac ccttgacgac cttgctgacg cggttgaccg cgatcatttt
    19321 ttcacgcatg ccgtcgtcgc gttcctgctc gacggcgtgc ttgccttgtg ctttagccat
    19381 ttgaattcct tgcttagaac ttcaggccgg cttcacgcgc ggcgtcggcc agcgccttga
    19441 cgcggccatg ataacggaag cccgaacggt cgaaggcgac ctgctcgatg ccggcggctt
    19501 ttgccttctc ggccacacgc ttgcccacca gaccggccgc ggcggcattg ccaccattgg
    19561 ccttctgacc ggcgagttcc ttgcgcactt cgggctcgag cgtggaagcg ctgacgagca
    19621 cgcggtcgcc ttccggcgaa atgatgtttg cgtaaatgtg cagattcgag cggaaaacgc
    19681 tgaggcgatg cacacgcagc tcgttgattt tccggcgggt cgctgtcgca cgacgcaaac
    19741 gggaggtttt cttgtccatg agtgttcctt gcttgcccgc ttacttcttc ttcgtttctt
    19801 tgagaacgat gcgctcatcg gcgtatcgca cacccttgcc cttgtagggc tcgggccgac
    19861 gataggcgcg gatctcggcc gcaacctggc caaccgcctg cttgtcggag cccctgacga
    19921 tgatttccgt ctgggtggga cattcgatct tgatgcc
//
LOCUS       DASTNK010000016         2626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1492376, whole genome shotgun sequence.
ACCESSION   DASTNK010000016 DASTNK010000000
VERSION     DASTNK010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492376"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1818)
                     /gene="poxB"
                     /locus_tag="VFR20_01195"
     CDS             complement(91..1818)
                     /gene="poxB"
                     /locus_tag="VFR20_01195"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007748458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HEU0229162.1"
                     /translation="MAAYTVASWMAELLANVGVKRVWGVTGDSLNGLNDSLHRAGKIE
                     WLGTRHEETAAFAAGAEAQLTGELAVCAGSCGPGNLHLINGLYDCHRSAAPVLAIAAH
                     IPSTEIGSGYFQETHPEILFRECSHYCELISNPGQAASVMAIAIRKAVIERGVSVVVL
                     PGDVALQPMPRSAPVTLTIPRPPVVAPRAGELARLADVLNEAGKVTLMCGSGCAGAHD
                     EVVRLAGMLKAPVVHALRGKEHLEWENPYTVGMTGLIGFSSGYHAMMSADVVVLLGTQ
                     FPYRAFYPRNATIAQVDIDGGSIGRHSHVDLGVVGDVGATLSALFPLIKQKTDARFLD
                     RALAHYAKTRAEFNALADPGKREKDLHPQYVAQRVSDLADDDAVFLCDVGTPTVWAAR
                     YLRMNGQRRLLGSFNHGSMANALSQALGAQKASPERQVVALCGDGGFSMLMGDFLTIA
                     AMRLPVKLVVFNNGVLGFVAMEMKAGGYLTDGTDLHNPDFAALANAVGVRGFRVERSQ
                     DLNNALTEAFAHDGPALVDVAIAKQELSMPPRTELQQAKGFSLYMLRAVLNGRADEVV
                     ELAQTQMFR"
     gene            1981..2289
                     /locus_tag="VFR20_01200"
     CDS             1981..2289
                     /locus_tag="VFR20_01200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEU0229163.1"
                     /translation="MIHVIAVITANPGQRDAVLTLFRQNVPNVHAEKGCISYEAVVDV
                     PSFGAPQAPLGSDTFAVIEQWENAEALKAHGASAHMAEYARKSAGLIARRVINVLQAC
                     "
     gene            2292..>2626
                     /locus_tag="VFR20_01205"
     CDS             2292..>2626
                     /locus_tag="VFR20_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0229164.1"
                     /translation="MAHYDVDLFVIGAGSGGVRAARIAAGHGAKVAIAEADRVGGTCV
                     IRGCVPKKLMVLAGRFADDFIDARGFGWEVGTPRHDWTALIAAKDKEIARLEGLYIQG
                     LTSSGVELIR"
BASE COUNT          465 a          902 c          811 g          448 t
ORIGIN      
        1 cccagactgt tcatggtcgc cacacgatga atcccccgcg cgcgtgaatc tccctgcgtg
       61 tggccccatg gacgccagtg ggaccacgcg ctagcggaac atctgtgtct gtgccagttc
      121 cacaacctcg tcggcgcggc cgttgagcac cgcgcgcagc atgtacagac tgaacccctt
      181 ggcctgttga agctcggtgc gcggcggcat tgacagctct tgcttggcga tcgcgacgtc
      241 caccagcgcg gggccgtcgt gcgcaaaggc ctcggtcagc gcattgttca ggtcctggct
      301 gcgctccacg cggaaccccc tcacgcccac ggcattggcc agcgcggcga agtccggatt
      361 gtgcaaatcg gtgccatcgg taaggtagcc gccggccttc atttccatgg ccacaaaccc
      421 caacacgccg ttgttgaaca ccaccagctt gaccggcaac cgcatcgcgg cgattgtcag
      481 aaaatcgccc atcagcatgc tgaaaccgcc atccccgcac aatgccacga cctggcgttc
      541 ggggctggct ttctgcgccc ccagggcctg ggacaacgca ttggccatgg agccatggtt
      601 gaatgaaccc agaagccggc gctgcccatt catgcgcaga tagcgcgccg cccagacggt
      661 gggcgtaccc acgtcgcaca ggaacacggc atcatcgtcg gcgagatcgc tgacgcgctg
      721 ggccacatac tgcggatgca ggtctttttc ccgcttgccg ggatcggcca gcgcattgaa
      781 ttccgcccgc gtcttggcat agtgcgccag cgcccggtcg aggaagcgcg catcggtctt
      841 ctgtttgatg agggggaaca atgccgacag cgtcgccccc acgtccccca ccactcccag
      901 atcgacatgg ctgtggcgcc cgatacttcc cccatcgatg tcgacctggg caatggtggc
      961 gtttctggga tagaacgccc gatatggaaa ctgcgtgccc agaagcacca ccacgtcggc
     1021 ggacatcatc gcgtgatagc cagaggagaa cccaatgagg ccggtcatgc cgacggtata
     1081 ggggttttcc cactccagat gctccttgcc tcgcagggca tgtaccaccg gtgccttcag
     1141 catgccggcc aggcgcacga cctcgtcgtg tgcgcccgcg cagccgctgc cgcacatcag
     1201 tgtgaccttg ccagcttcgt tcaggacatc cgccaaccgg gccagttcgc cagcccgggg
     1261 cgccacgacc gggggacgcg gtatcgtcaa cgtgacgggc gcgctcctgg gcatgggctg
     1321 cagcgccacg tcgcccggca gcaccaccac cgacaccccg cgctcgatca cggctttgcg
     1381 gatggctatg gccatgaccg acgcggcctg ccccggattg gagatcaact cgcaatagtg
     1441 gctgcattcg cggaacagga tctcgggatg ggtctcctgg aaatagccgc tgccgatctc
     1501 ggtggacggg atgtgcgcgg caatggcgag caccggcgcc gcactgcggt ggcaatcgta
     1561 caggccattg atcaggtgca ggttgcctgg cccgcatgac cccgcgcaga cggccagctc
     1621 accggtcagc tgggcctcgg ccccggcggc gaacgcggcg gtttcctcgt ggcgcgtacc
     1681 cagccattcg atctttccgg cgcggtgcag gctgtcgttc aggccattga gcgaatcgcc
     1741 cgtcaccccc cagacccgtt tcacccccac gttggccaat agctcggcca tccatgatgc
     1801 gacagtgtat gcagccattg cggcgtctcc tggaatgaat tcagcgttgc gaaactcctg
     1861 cgcgggcaac ccgggacggg cgccccgttt cgattttaag tcgcccggcc cgccgggcac
     1921 caccggcgga aatgcgtcta tgatgaatgg cgtaccactt tttccggaga ctcatccgcc
     1981 atgatccacg tcatagccgt catcacggcc aatcccggcc agcgcgacgc cgtactcacc
     2041 ctgttcagac agaacgtccc caacgtccac gcggaaaaag gctgtatcag ctatgaagcc
     2101 gtggtcgatg tgcccagctt cggcgcaccg caggcgcccc tgggctccga cacattcgcc
     2161 gtcatcgaac agtgggagaa cgccgaggcg ctgaaagcgc atggcgcgtc ggcgcacatg
     2221 gccgaatacg cccgcaagag cgccggcctc attgcccggc gtgtgatcaa cgtgctgcag
     2281 gcctgctgat catggctcac tacgatgtcg acctctttgt catcggcgcc ggctcggggg
     2341 gtgtgcgcgc cgcgcgcatc gccgctggtc acggcgccaa agtggcgatc gccgaagccg
     2401 accgggtggg cggcacctgc gtcattcgcg gctgcgtgcc gaagaaactc atggtgctgg
     2461 caggccgctt cgccgacgac ttcatcgacg cccgcgggtt cggctgggag gtcggcacgc
     2521 cccggcacga ctggaccgcg ctgatcgcgg ccaaggacaa ggaaatcgcc cgcctcgagg
     2581 gcctgtacat ccaggggctg accagcagcg gcgtcgagct gatccg
//
LOCUS       DASTNK010000017        47426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_752670, whole genome shotgun sequence.
ACCESSION   DASTNK010000017 DASTNK010000000
VERSION     DASTNK010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 47426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47426
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752670"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..77)
                     /locus_tag="VFR20_01210"
     tRNA            complement(2..77)
                     /locus_tag="VFR20_01210"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42..44),aa:Asn,seq:gtt)
     gene            complement(241..2076)
                     /locus_tag="VFR20_01215"
     CDS             complement(241..2076)
                     /locus_tag="VFR20_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEU0229165.1"
                     /translation="MEEILLVERSFERTARQLTQRLLADPRAGSVVDLWLFEGPVRRR
                     RMEAELAQAGIRARVFSAYKPLLHYFLEHVDRGTLKTARIVYPRHTACPENRFLLETH
                     PLVDLFPMARIDLVPATTTADADLPAADNGSTGTVTPLAYDVTLGYASGRSETLHIPT
                     PNVVGNDFLGQPFYTPSAWMVAINGNVTRSEPLAADYSLAYQAAMAAVINHDWGRAEP
                     YFDRLTISVRLPCEEQRLDCGDETISLAEALHEELYFSILEYFQQYSGRPPGNRGLQP
                     GQIVPDITIDNNAESIRVTVTTTPTPTQHRAPKQLPAVDTRDIPGKTVALPAAVSPLP
                     FNQIEKALQAYQGRHITFASSQGRPVNVVHVPGHLPAVLISAAQHANETSGVVGALRA
                     TGRLAARPDAHFVLMPVENPDGYALHQALCATHPTHMHHAARYTALGDDVEYREHAPW
                     YERLARNHAFETSGAQLHLNLHGYPAHEWTRPCTGYLPRGFEIWSLPKGFFLILRYKA
                     AWRDTAFALLRHVAQSLAGNPRLRAFNEEQLRRYERHSGHMPFLLEAGVPYTAVEAPN
                     QTPGVTLITEFPDETLHGEAFVFAHSVQAQTVEAACDWWWANVRQ"
     gene            complement(2200..3786)
                     /locus_tag="VFR20_01220"
     CDS             complement(2200..3786)
                     /locus_tag="VFR20_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEU0229166.1"
                     /translation="MTSETSSKAKAVAPKHVSYPPLEGKARLFGTIALSAAVFMNVLD
                     SSIANVSIPTIAGDLGVSATQGTWVITSFAVANAITVPLTGWLTQRLGQVRLFVLSTL
                     LFVLASWLCGFSWSLGSLVMFRVIQGAVSGPMIPLSQSLMLASYPRSKAGMALALWSM
                     TVLVGPVAGPLLGGWISDNYNWPWIFYINVPIGLVAGWASWQLYKGRESVKSKLPIDK
                     LGLSLLVVWVAALQILLDKGKDLDWFSSGLITTLAVIAVISFVFFVIWEITEKHPVVD
                     LTLFKELNFTSGTITLSVAYGVFFGTNVLLPLWMQNTLGYTATDAGIASAPVGILAII
                     LSPIVGKLLSKYDSRYISTISFLIFALISYMRSRFNTDVDIYTIMVPTFIQGAAMAAF
                     FIPLTTLTLSGLDHHRLPAAAGLSNFVRLLCAAFGTSITTTIWENRATLHHAQLTEMA
                     KPGEPAFDAITGMLAHHGLSHTQSAGLINGIINEQAFTMSAADFFYASAILFVVLIAL
                     IWIARPESPGSGSASAAGAH"
     gene            complement(3935..5119)
                     /locus_tag="VFR20_01225"
     CDS             complement(3935..5119)
                     /locus_tag="VFR20_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEU0229167.1"
                     /translation="MTTEHKSAPKRKRLLLTAALIFIVIGVCYAIWWVVVASHYETTD
                     DAYVHGNLVQVTSQIAGTVVGIDADDTEHVDKGQPLIKLDPTDTEVALKQAEAALAQA
                     VRHTRTLFVQNDALKSDIAVRQADIERAKVDLAKAKSDLARRRMLAKSGGVSGEEILH
                     AETAEKAAESSLAQAHAALAVARAKLETNQALTSGTTVAKHPDVRQAADRVRNAWLAN
                     ARTVLPAPVGGMVAKRSAQVGQRVAPGTPLMTIVPLKQVWVEANFKEGQLARMKPGQS
                     VTMTSDVYGSDVVYHGTLVGIAAGTGSAFALLPAQNASGNWIKVVQRVPVRITIDPKD
                     LVKHPLRVGLSMAVEVDLASPAKKPDDAQADTKLETNAFQANAIGANALINKIIEANL
                     GS"
     gene            complement(5138..6598)
                     /locus_tag="VFR20_01230"
     CDS             complement(5138..6598)
                     /locus_tag="VFR20_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEU0229168.1"
                     /translation="MKPLIPILLALTLTGCAAMDPGNPAVKEIQGTQLGLSGASIKWP
                     ARQWWHRYQDPQLDTLVSEALNKNPSLAAAQARLRIANEAVHGARAVQLPNLNATYNQ
                     TRERLSENYIYPPPLGGSMQTDVNLQLNLGFDLDLWGKQRSLYAAALSRDEASRADTE
                     VARNALISAVTQSYFNLQDALEQQEVIGKIVKQLENVASITRQRLKAGLDTRVEVNQA
                     DSAVSAAKVQLSQASTNASLLRHQLAALVGAGPDRGMTIQRAKLAHAPTGIPKIIPMN
                     LLGRRPDIIAAKLRVEAASSNISAAKAGFYPDINLSAFFGFQALGLGNLLHDSSKTYG
                     VGPAISLPIFNGGALNAQLGTKRAERDLAIANYNQTVLTAVRQVADATTAIRDLRQQL
                     IDQHASYESIKSAYEIAVKRYQSGLGNFLQVLLAQNDVLKQAIQDTRLRARAYNLDAQ
                     LATALGGGYGPAETPKTMTTQNGGATAATTTTVESH"
     gene            complement(6735..7343)
                     /locus_tag="VFR20_01235"
     CDS             complement(6735..7343)
                     /locus_tag="VFR20_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0229169.1"
                     /translation="MPKNTPNSSRARPARPSASAPDASPASAPTSGSDQNVAAKTPAA
                     FYRADVASLRSKNNLGRLLKQSYTSLNRMIDGYVAPMGLTAMQWKPLVIICHQNTNTP
                     AELARRATVDTGAMTRTLDRLEAKGFLRRQRCDTDRRVVNLELTEAGRDVVNDILPAV
                     AKAFNNHLAGFTDAEIETLLNLLGRLIDNGSTESEPDFPDQS"
     gene            complement(7553..8758)
                     /locus_tag="VFR20_01240"
     CDS             complement(7553..8758)
                     /locus_tag="VFR20_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU0229170.1"
                     /translation="MTTPRKTPAVHDTVRPALPPYETVALVLQGGGALGAYQAGVYQG
                     LAEAGIEPDCLSGISIGALNTALIAGNPPEKRVARLTEFWETICQPNLGLAMNPFWEQ
                     SLFNMNDTVRRNMSALHAAGAVLGGQRGFFRPRMPPPAMTTPGDPGTASYYDTHALHD
                     TLERLCDFDLINAGTHRVSVGAVNVRTGNFVYFDNARTRLEPRHFMASGALPPAFPAI
                     EIDGEYYWDGGLVSNTPLSYVLESRPQKDTLAFQVDLWSARGRAPANMSEVSDRVKEI
                     QYSSRTRLITEDMHRLQHLRHMLAYLLDKIPPDQRAQDRYAHLAEEMSCLKRYNVVHL
                     IYRDKPYEQYYKDYQFGLATMRDHWASGLADIRATLAKPEYLRMPDNVSGFITHDVHR
                     HDIEAESEG"
     gene            complement(8755..9660)
                     /locus_tag="VFR20_01245"
     CDS             complement(8755..9660)
                     /locus_tag="VFR20_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0229171.1"
                     /translation="MIGISIRQLEVFVAVASAGSVRAAATRLSVTQPAVSMALSELER
                     QIGAPLFDRIRGRLHLSTRGKELLPAAQEVVERMHEMLREPDEHTHALSGELRVGASN
                     TIGNYLVGELLGAFVRRHPGVSLSVSVENTRTTVAGVLEHRIDVGCVEGPVNHPEIDT
                     LPWRNDDLQVCAAPDHPLVRRRRLRPSDFAEAAWILREPGSAMRTQSETLMASLPPGR
                     ILLELGQVEAIKQAVIAGLGIACLPGAATVDAVATGRLAVLDTPFLALQRRLSLILHK
                     ARYRGALIEAFVRSLEPNLTTWSDS"
     gene            9699..11261
                     /locus_tag="VFR20_01250"
     CDS             9699..11261
                     /locus_tag="VFR20_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0229172.1"
                     /translation="MRSVQNVLFIMADQLRADHLACYGHPRIRTPHIDALARRGVRFE
                     RAFVNSGVCGPSRMSYYTGRYPSTHGATWNRVPLSVGELTLGDYLREHQLTLALAGKT
                     HVMPDNAGLARLHLDGGSELGRLLRAGGFEEVDRYDGHSKPGAESGYPAYLRRHGYAS
                     DDPWSDFVISARDASGQVVSGWHMRNAGLPALVDERHSETAYMTDQALAFMKKKGDEP
                     WVLHLSYVKPHWPYLAPAPYHALYGVDDCLPVVRNEAEKQDAHPVVAAYRQQEESQSF
                     TLDECIRAVRPTYQGLITQLDDHLGRLFDYMEGAGLMRNTLVILCSDHGDFLGDHWLG
                     EKELFYDVVQRVPLIVVDPSAAADATRGRAESRLVESVDIVPTVLESLGIPLPWHRLE
                     GRSLSPLLHDQSVAWRDCVFSELDYSYRLARVLTGKTPFNARGWSARTDRWLYVYWLD
                     EPEQLYDLRDDPDQFHDLGRDPDHEEARRAMKARLFDWFAGLKHRATVTAESVEKGTN
                     AYKKAGVFYGQW"
     gene            complement(11361..11735)
                     /locus_tag="VFR20_01255"
     CDS             complement(11361..11735)
                     /locus_tag="VFR20_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEU0229173.1"
                     /translation="MYFQLVKHLHISAATLSIIFFVVRAWWSVTGSSRLQLRIVKVLP
                     HVIDTVLLVCGIILTVMLGGLQTWIVAKIIALVFYIGVGTVAIKRGKTASTRAVAALI
                     AIAIFFYIVGVALAHNPMSWLT"
     gene            complement(11816..13780)
                     /locus_tag="VFR20_01260"
     CDS             complement(11816..13780)
                     /locus_tag="VFR20_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEU0229174.1"
                     /translation="MDWNHQLIDSAIWLLKAYIITAILFCVAVWAVARFTVWGRQFWD
                     ISGAYFTSRRNWRPVAVVAVILLLTLGAVRLDVLFSNWYNSMYSSLQKLDEAGFWFAM
                     LVFGILATTHVIRSLIEFYVQQAFTIHWRVWLNERLLSRWLEKRAYFRTQYLDTPVEN
                     PDQRIQQDVAGFVQTSLSLSMGLVNALVSTFAFTLILWNLSGPLNLFGVSIPRAMVFL
                     VFVYVIIATYFAIRIGRPLILLNFLNERFNADYRYALVRMREYAESIAFYAGERVEGA
                     LLRSRFGQVISNAWAIVYRSLKFLGFNFAISQTAVVFPFILQAQRFFTKQITLGDLIQ
                     TAQAFGQLQSNLSFFRNAYDSFAAYRATLSRLTGFSSAIIESYSLPEPDVREEGALIA
                     LRDLTITTPAGRVLLERLTLEARANTPLLIRGPSGAGKTTLLRAVAGLWPYCSGDIIR
                     PEHSALFLSQKPYLPLGSLRDALYYPNQVLHDGMATPASEGNLEQDLARAGFTRKTLD
                     RQSHALPEVSFRTADALSSPTATNALVPINDPPPVDTQGKAAEILTRIHLGHLTDRLD
                     EVADWGRILSLGEQQRLAFGRLLLAAPLAAFLDEATSAMDEGLEDAMYRLVRELLPNT
                     ILVSVGHRSTLVPHHVRQLILQGEGRWVLQ"
     gene            14006..14380
                     /locus_tag="VFR20_01265"
     CDS             14006..14380
                     /locus_tag="VFR20_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEU0229175.1"
                     /translation="MPYVTITSSRGLSTEQKKNLLQHSSDAVVQSIGAPLPSVRVMLH
                     ELPDGHYLNGGSFDTRALMFEVDFIIGRTDDQKAALVAALSHAGSNVTGVPENEVRVR
                     LVDFPKTNMGMAGGVTADRLGR"
     gene            14512..15129
                     /locus_tag="VFR20_01270"
     CDS             14512..15129
                     /locus_tag="VFR20_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEU0229176.1"
                     /translation="MTQDAQPLRWVAPASDPADANVSKPGSLSEARAVVDFWRDAGPK
                     AWFSKDPLFDQAFRSRFSELHFAAARRERDHWLSDPYACLSLILLLDQFPRNAFRGTG
                     HMYATDSLARCYARAAVNFKHVKHLEDVLRPFVFLPFMHSESLEDQRYCVDLYRDYAP
                     QNLTHAQEHCNIIQRFGRFPHRNVQLGRATTREEQRFLDNGGFSG"
     gene            complement(15225..16520)
                     /locus_tag="VFR20_01275"
     CDS             complement(15225..16520)
                     /locus_tag="VFR20_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEU0229177.1"
                     /translation="MTSTTESPSNQALTPLDGTHRVFGWTQHASLWFSLGVGLLVMQV
                     GAYLVPAAGTREALFAIIAGSLIGSGLLAWVARAGCESGLSSAGLIHATYGHSFSRLP
                     VLLNIVQLLGWTTFELVIMREGTVNILVKALGPGFGGVAAYVAATVFWGLVLTALLAG
                     SMITLVRAIISRAALPLVVLSLLWLSWQFAGKLHADSLATIWNRRGDGTMGMFSALDL
                     VIAMPVSWLPLVADYARHGKSGRGALGGTWLGYTLANAWCYALGMLVVSVAQPGTDLV
                     SALLLAQGGLVALGLILVDELDNAYGDVYSGSVCAHSIEPRWSIRRWGLTLALASTLL
                     ALVLPMHSLEPFLLLLSSVFVPLYGVILGRLGLTQPSLTTHGPKIDKVAAALWLLGIA
                     VYHGLAQWAPAAGSALPTLALTFTLAALTRPTGRLAAAG"
     regulatory      complement(16578..16674)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(16749..17534)
                     /locus_tag="VFR20_01280"
     CDS             complement(16749..17534)
                     /locus_tag="VFR20_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEU0229178.1"
                     /translation="MSFTLLPSEPGQAQHARFDEFLMRRAAQGSPVACIWRTGQGLVV
                     PRTYHRLPRFAEACRRFARLGWPVTVRQSGGGLVPQGPGILNISLAYMAAGQPLSQSD
                     AAYQSLCALISGALNDLGIATRPQAVTGSFCDGRYNLAWLDDSGTARKIAGTAQLWRR
                     VAPEGDTPTIRDAPGRTDAVQVVLAHALLLAAVDTDAVTRIANGFERASGHDKHYDPD
                     TISSLHAILGIAADASNVFMTDLTNGLAQHIAAASPPASTQTP"
     gene            complement(17547..18023)
                     /locus_tag="VFR20_01285"
     CDS             complement(17547..18023)
                     /locus_tag="VFR20_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEU0229179.1"
                     /translation="MLQKAVLPIVIFVIILIALTFGESLSHDLFAWISHITGLVIYNF
                     ADLYRAVAGYIDGHTGRVVAALVLTVPVTWWIMKNKGSELDKPGSRRKMAIVLAIFLG
                     WLGGHRFYLGQVGMGILYLVILYVFTPLVVVLSLIDAVRYLFMSDSDFTASGSAVM"
     gene            complement(18121..19272)
                     /locus_tag="VFR20_01290"
     CDS             complement(18121..19272)
                     /locus_tag="VFR20_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0229180.1"
                     /translation="MTPRTNPPPQPYISWQEGNETRQALWCVPGDPPHPDHVRVVDDT
                     LTADQAFRLASQGTAMLWRGDYRNARQLLVALAHRVDHRPRKRKQPDSPHDAFNQHRL
                     AQSQRATQLNQLLIPMEGDGAVALRHAPDVRAACRDALGDVGEPFVLPLRAVQGMVGA
                     HEWRRKGVAVPVLDEPIHVHYGVFSPIRGEYLDLIAQTPLPATDLAFDIGTGSGVLAA
                     ILARRGVARVVATDSDPRALACARENVETQGLTGRVTVQEADLFPSGACGLIVCNPPW
                     LPARPTTSLESAIYDPGSRMLRGFLDGLADHLLPEGEGWLILSDLAERLGLRAPDFLA
                     TAIGRAGLRVVGRLDTRPRHPKVLDTSDPLHAARRDEVTTLWRLTVAHP"
     gene            complement(19288..20181)
                     /locus_tag="VFR20_01295"
     CDS             complement(19288..20181)
                     /locus_tag="VFR20_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0229181.1"
                     /translation="MSRSLNFQQIQAFRAVMQMGTTTRAAAMLNTTQPSISRRIAELQ
                     AAAELKLFDLHHGRLRPTSEGKLLYKTIQKHFDGLEKIESVVAIMRKSGSGVLRVGCT
                     PTLAIGLLPQVINKFLSQYPRTYITIQTLSTLQLADYLHQDLVDLVLTTGTLDQNDFR
                     PSTIRTSSAVCVLPMNHRLRKRSIIDIGDMAGERILTLSDTDELTIKARSLLSEHGIA
                     DDFVIETPSSITVCALVAAGNGIGIVNPYVANTFSGQLLVRDITPRIPVHVQMAMPAQ
                     TAPSALTRHFTDVLKSHIATM"
     gene            20402..21679
                     /locus_tag="VFR20_01300"
     CDS             20402..21679
                     /locus_tag="VFR20_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229182.1"
                     /translation="MNPRNIVDKPCPCRRLGENAGAHHFFGYYNKTVWDRTGRYVLAN
                     RTAFMDADLTPDLSAGIGFFDPLNGDAFLEVDSTKAWNWQMGCQLQWLDGRPQRQIIY
                     NIRTDAQTGPYPGFGSRIHNVDTNEVQDLPLPIYVVSPDSRHALCVDYSRLYVTHETI
                     GYPPRPSVLPNAPDDDGIRRLDLATGRHELILSYGDLFRFHHRPSMDKALHWVSHIEI
                     NPDSSRVVLLHRWTERVEDETCFLHRLITMDLDGSNLRLLECSDHPLPQLADDFDPHA
                     VGTFDYEKSPYQISHPAWRDNDHIVVWSPHKGEIHYHLYHDRDDGDVCVLSPGILTEN
                     GHMTFSPTDKRWLLSDTYPDAVTNERILFLLNVDDNVRYDIGRFYADPNLRKENRCDL
                     HPRWSRDGRSVCVDSVHESERQMYLLDVSTIVG"
     gene            21789..22727
                     /locus_tag="VFR20_01305"
     CDS             21789..22727
                     /locus_tag="VFR20_01305"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU0229183.1"
                     /translation="MINSTSRQCGRRRVVLSSLFLAVACAIGLGSANAADAPLTGTLK
                     TIKDNGVVVIGFRDASIPFSYYDSNQQPIGYSMDFSNLIIDKLKQDLKMPNLKVRKIP
                     ITSQNRIALLQNGTFDFECTTTTNNEAREKQVDFSDSFFEIGTRLLTLKNSGIKDFAD
                     LKGQTVVVGAGTTSEKILRRMNVEKKMGMHIISAKDHTESSLMLKTGRAKAMMMDDAL
                     LAGERAKMKTPSDYVITGTPQSHEVYGCMTRKNDEGLKKLIDGVVSKAETSGEAAKIY
                     KKWFMSPIPPKGLNLDFPMSDDMKALFAHPSDKPVS"
     gene            22779..23516
                     /locus_tag="VFR20_01310"
     CDS             22779..23516
                     /locus_tag="VFR20_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU0229184.1"
                     /translation="MDYKWNWAVLLQPVATGEPTTYLGWLASGFMNTVYLTLLAWLLA
                     LAVGSAFGVMRTLPGRIPRVLGTAYVSVFRNVPLIVQFFIWYFVVPELLPKALGDWIK
                     NLDPNTQFFVVSVFSLGIYTGSRLCEQVRAGIQSLPKGQRNAGLAMGLTLAQTYRHVL
                     LPVTFRVIIPPLTSEFLIISKNSAVASTIGLLELSGQARQLVDYTAQPYESFICVTLG
                     YFLLNFLILRLMDWIRRRTSVPGFLGS"
     gene            23518..24198
                     /locus_tag="VFR20_01315"
     CDS             23518..24198
                     /locus_tag="VFR20_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143216.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0229185.1"
                     /translation="MFQMDWKTLFESRDLLLTGMWVTVRITFIAILCGLAWGTILAVF
                     GTVRNKALNLFSLWYVSLFRSIPLVMLLLWFFLIVPQFLKALFDLPPTVDIRLVSAMV
                     AYSLLEAAFFCEIMRAGIVSLPKGQPAAGYALGMTPYQCMRYVVLPQAFAAMLPIVLT
                     QCIVIFQDTSLVYVIALGDFFSRAESIGARDGTIIPMLIFAGVVYWVICSSLSGVVRL
                     VRARRPGL"
     gene            complement(24208..25704)
                     /gene="glpK"
                     /locus_tag="VFR20_01320"
     CDS             complement(24208..25704)
                     /gene="glpK"
                     /locus_tag="VFR20_01320"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU0229186.1"
                     /translation="MAYLLAFDQGTSSSRSIVFDTDTGRIVALAQQELTQHYPRPGWV
                     EHDPMDIWSTQLRTARDALAKAGLQARDILAAGITNQRETTIVWHRETGQPLCNAIVW
                     QDHRAEPACADLRQRGLEDTILQKTGLRVDAYFSATKLKWILDNISGGRELAARGKLA
                     FGTVDTWLIWQLTKGRVHATDVSNASRTMLFNVHDNQWDAELLRTLDIPHSLLPDVRP
                     SSTEYGTVDTELFGHAIPICGVAGDQQSALFGQACFQEGMAKNTYGTGCFTLMHTGAK
                     FQVSKNGLLTTSAAQTTSQPEYAIEGSVFVGGAVVQWLRDGLRAIPSSGDVEKLARSV
                     PDSGGVMVVPAFTGLGAPYWQPDARGSITGITRGTNVGHIARAALDSIAYQSAALLQA
                     MSRDAVQAGAAPLAELRVDGGACVNDLLMQFQADLLGIPVVRPTIIETTALGAAYLAG
                     LACGAFTSKEALSRLWRVERTFTPRLDRREAEALMQRWEHAVRQTSLP"
     gene            complement(25733..26503)
                     /locus_tag="VFR20_01325"
     CDS             complement(25733..26503)
                     /locus_tag="VFR20_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEU0229187.1"
                     /translation="MTPNPRQQRLLRHVQEQHSSTVEELAIRLGVTAQTIRRDVQRLA
                     EAGLLARFHGGVSVPSSTTENIAHHQRENLNTSGKRRIASAVAQAVPDECSLFINIGT
                     TTEAIARALMHHTGLRVITNDLNVAATLSRNRNCEVIVAGGVVRSRDGGIIGEATIDF
                     IRQFKVDIALIGISGIEPDGSLRDFDYREVKVSQAIIEHAREIWLAADATKFNRQAMV
                     KVADLSQVDRLFTDATPPDPFPDLLAQVHVRCDVAGSP"
     gene            26718..28319
                     /locus_tag="VFR20_01330"
     CDS             26718..28319
                     /locus_tag="VFR20_01330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEU0229188.1"
                     /translation="MMPASEPLLTDRSRLLSALSEPMRYDLAVIGGGASGLGVALDAV
                     LRGLSVVLLESHDFAKGTSSRATKLVHGGVRYLAQGNVSLVREALRERTVLLHNAPHL
                     ARPLPFVMPCYSYWEMPFYGSGLKLYDLLAGKAGLGNTEMLGVAGTERCLPGVRTRGL
                     KGGVKYWDGQFDDARLALTLARTAAARGALLVNYCAVTGLVHEQGRIAGLRAKDAETG
                     AEFQISARCVVNAAGVWVDEIRQQDGQAMHRAAKPMVAPSQGVHLVVDRDFLRGNHAL
                     LVPKTRDGRVLFAVPWLGKVVLGTTDTPRHDLSREPDAFANEVDFILGEAGNYLSRRP
                     TRDDVRSVWVGLRPLVRPPDDEGDNTKKISREHTVLVSPSGLVTVTGGKWTTYRAMAE
                     DVLAKCVEAGLFSSLPPSVTATCVLAGAVAGATYRATLTETPGAEAYGEDAQWLRGLD
                     GHDVTLADGLTESMVRFAARYEYARSVEDMLARRYRLLFLDARLAAHVAPRVAELLRE
                     ELGVDPRLDDFLRLADHYLRLPAGS"
     gene            complement(28395..28913)
                     /locus_tag="VFR20_01335"
     CDS             complement(28395..28913)
                     /locus_tag="VFR20_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEU0229189.1"
                     /translation="MPQYVRRFLFPGLVAVLTLLAGCASTPRHESGAGYWRGQPVVVD
                     QSKRTDVVMMALAQLDTRYRYGGDSPDTGFDCSGLVDYVFKAAADAGLPHNTARIAEI
                     SRPISRRALSPGDLVFFNTLNRRYSHMGIYLGHGRFINAPSSGGVVRIDSLNSRYYAR
                     RFDGARTLFAAD"
     gene            29446..29772
                     /gene="flhD"
                     /locus_tag="VFR20_01340"
     CDS             29446..29772
                     /gene="flhD"
                     /locus_tag="VFR20_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HEU0229190.1"
                     /translation="MLAVDDSLLSDIQEVNLSYLLLAQRLLRENYASAMFRLGFTEDV
                     AEVVLQLSPAQLVKLAGSGSLICGFRLNDYDLLSSLANDVLGGVLQQAHATILLAQRS
                     ALPQVA"
     gene            29798..30358
                     /gene="flhC"
                     /locus_tag="VFR20_01345"
     CDS             29798..30358
                     /gene="flhC"
                     /locus_tag="VFR20_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HEU0229191.1"
                     /translation="MSTKSVSRDAEEILLATDMIKLGARLQVLQAETSLSYDRLARLY
                     REIKGCSPPKGMLPFSVDWFMTWLPNIHSSVFYNIYRFMDAHTPAKKSGALIEGYRLY
                     LEQADAGRLASSAKEPVLSFTRAWMLVRFFDSDLVQLSRCTRCTGQFVAHAHDPQANF
                     VCAICRPPPRAGKTRGRAVLRQPAKV"
     gene            30531..31406
                     /gene="motA"
                     /locus_tag="VFR20_01350"
     CDS             30531..31406
                     /gene="motA"
                     /locus_tag="VFR20_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HEU0229192.1"
                     /translation="MLILLGFLVVLVSVFGSYTFLGGHLGALYQPFEFVLIAGAALGA
                     YLASNSGKSIGLLLRAVPNAFRRSPYDKPLYMELMALLYVLLNKARRDGLMSVESDIE
                     DPSASPVFAEYPLVLRNARLMEFITDYLRLIISGNMSAFEIETLMDQEIETLRKELHI
                     PVHGLRAVADALPAFGIVAAVLGVIKALAAVDQPPAILADLISKAMVGTFLGILLAYG
                     FVAPMASSIERRNAGVEKVLECIKLTLLANMNGYPPQLAVEFGRKALYSTVRPSFVEL
                     EEHVRQTRSLAGKKA"
     gene            31419..32333
                     /gene="motB"
                     /locus_tag="VFR20_01355"
     CDS             31419..32333
                     /gene="motB"
                     /locus_tag="VFR20_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEU0229193.1"
                     /translation="MSSRGPERIVIRRGHAHLDESHGGSWKIAYADFVTAMMSFFLVM
                     WLISVVPHKDLHAIAEYFRMPLMTAVTGGPKIDSSSSVIPAGSPSVIPNKNPLPRQQH
                     DDGTTESDKRDTQRLEGLKSELDQLIKSDPILKQFRPQLLLDMTPDGLRIQIVDQQNR
                     PMFDTGSAKMQPYMSAILRELAPVFNKLPNKISIAGHTDAAQYSTGEREYSNWELSAD
                     RANAARRQLIAGGMDEGKVKRVLGLGSTVSLIKDDPSAAVNRRISLVVLNKRAERRID
                     AQNTTAAAVLKLPELEHLAQPALAAPSD"
     gene            32361..34436
                     /gene="cheA"
                     /locus_tag="VFR20_01360"
     CDS             32361..34436
                     /gene="cheA"
                     /locus_tag="VFR20_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEU0229194.1"
                     /translation="MSAGVDLSQFFDTFFDEADELLADMERLLLGLDVDQPDSDDLNG
                     IFRAAHSIKGGAGTFGCFDALTQTTHLLENILDQVRRGDMALRKEMIDLFLETKDVLA
                     EQVAAYRSSGNPDAQAYERICAQLRELALANQTDGVDDGVPAMQQAGPLAPAPASTSV
                     VAVAVSAVAGGKSVKRVDLTGVASADAQSLITEMALLGEVVRHEDRGGVVSIWLETPE
                     SADDITAVCCFVVNADQVRVTSEGGGFAAEPVVGAQHPPAPPVAGAPAPGSPLAPEPT
                     SAGHEGREERRSGVDRRKTTDSAARKESGTIRVGVEKVDQIINLVGELVITQAMLVQA
                     GSELDPVVHDRLLNGIEQLERNARDLQEAVMSVRMMPMDYVFSRFPRVVRENASRLGK
                     RIRLVTQGEATELDKSLIERIIDPLTHLVRNSLDHGIEAPDVRLARGKNPEGCITLSA
                     QHNGGQIIIEVSDDGAGLDRRRILAKAASQGMAVSDTASDEEVWQLIFAPGFSTAEKI
                     TDISGRGVGMDVVRRNIQDMGGHIQLSSREGRGTIMRIALPLTLAILDGMSVKVGNET
                     FILPLSHVTESMQPTTEQIHHISEGEQLMYVRGEYLPIVALHEVFSVEGAQTNPTGAI
                     AVILQAEDARFALLVDQLVGQHQVVVKNLESNYRKIPGVSAATILGDGSVALIVDVFA
                     LMRMTRETA"
     gene            34463..34963
                     /locus_tag="VFR20_01365"
     CDS             34463..34963
                     /locus_tag="VFR20_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEU0229195.1"
                     /translation="MLNRFESDAGQVDTVGKEFLVFTLASQEYGIDILKVQEIRGYDS
                     QTVTRIANVPSFVKGVTNLRGVIVPIVDMRIKFDLERVEYDHQTVVVILNLGSRVVGV
                     VVDGVSDVLMLQSSQISTPPQFGTAFSTEYLTGIGTLGERMLILVDIERLMTSTEMAL
                     VEQAVA"
     gene            35034..36770
                     /locus_tag="VFR20_01370"
     CDS             35034..36770
                     /locus_tag="VFR20_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU0229196.1"
                     /translation="MRKPVLFSNFKVRTSLILMLLFFLCMLLSGAALGLWSLYSNNQA
                     LQRIADNEAIDSTLSQSIDQYKDIQGALGRALASYVVNHDKRHSGAAGQAEVAATTYT
                     GALDPESQSQVKNARQAMSTSQSIFDTYRRLTKGRKDGVYRDLEQSYDALMAGGVTPL
                     FGFLAKGDISGYHDFLSTTSSSLEDDFYQTLGVVHAHQRQLIADSEAAEQMHYQRVVI
                     LVAIAVTLSVLIALLAYAVLGRVVLRPLRAAQSHFERIAAGDLTQRIEVRSRNEIGVL
                     FDALRRMQESLTGTVSSVRQGVEEITLGSQEIFAGNTDLSSRTEQQAASLQQTAASME
                     ELASTVKQNTDNAMQADQLAKKASEVAQRGGAAVGDVVGSMAAISASSGKISEIVGVI
                     DGIAFQTNILALNAAVEAARAGEQGKGFAVVAGEVRSLAQRSAQAAKEVKTLIEESRS
                     RVQAGSAQAIQAGDIMREVVGAVEGVTTIMSEISSASREQSDGIGQVNQAVAQMDEVV
                     QQNAALVEQAAAASGSLQDQAARLNDAMAVFKINTSEIIDVPAAALAQPDKPGGRMAA
                     AYAPATLAGQIS"
     gene            36767..37621
                     /locus_tag="VFR20_01375"
     CDS             36767..37621
                     /locus_tag="VFR20_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEU0229197.1"
                     /translation="MSAMPRAESFSLLAGLPEAGDDDITLSTRLLKARAGLVLGAHKR
                     DMIGRVLGLRARDLGVGTVTDYLTSLQRGGESTEWEHFVNAFTINHTAFFREQHHFDS
                     LTKFARACKTPISVWSCAASTGEEAYSIAMTLREAQPATDLRQAVWATDIDTQAIARA
                     RAGVYSGDRAEPIPEVLLKRYFQRGQGARTGQVRVKPLLSSLVRFDTFNLCAPAWPSG
                     RMFDAIFCRNVMIYFDKETQVRILDHMATMLRPGGLLFAGHSENFTYLTDKFRLLGQT
                     VYERDKLK"
     gene            37618..38712
                     /locus_tag="VFR20_01380"
     CDS             37618..38712
                     /locus_tag="VFR20_01380"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEU0229198.1"
                     /translation="MISADRQAPGQTAVSGKIRVLCVDDSALVRGLMTEILNGQPDIE
                     VVAVAPDPLVARELIKRHNPDVLTLDVEMPRMDGLDFLERLMRLRPMPVVMVSSLTER
                     NSDVTLRALELGAVDFVTKPKLGLRDGLLEYSDLIADKIRAAAQSRPRQRQPVQAVAV
                     RRLARPFSTTEKLILIGASTGGTEAIRQVLEPLPANSPAILITQHMPAGFTRSFVRRL
                     DSLCAVQVHEAEAGQRVLPGHVYLAPGGVAHMRLSRSGANYVVDLDDTEPVNRHRPSV
                     DVLFHSAAQVAGGNAVGAILTGMGRDGAQGLLAMRKAGAMTFAQDEASCVVFGMPREA
                     LLIGAAAEAVPLAEMGERLLASAGAYGHRV"
     gene            38766..39155
                     /gene="cheY"
                     /locus_tag="VFR20_01385"
     CDS             38766..39155
                     /gene="cheY"
                     /locus_tag="VFR20_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HEU0229199.1"
                     /translation="MAQKSIKILVVDDFPTMRRIVKNLLKDLGYENVDEAEDGAMALE
                     KLHTGNFDFVVSDWNMPNMDGLSMLKAIRADEQLARLPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKHQI"
     gene            39183..39827
                     /gene="cheZ"
                     /locus_tag="VFR20_01390"
     CDS             39183..39827
                     /gene="cheZ"
                     /locus_tag="VFR20_01390"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HEU0229200.1"
                     /translation="MHTAESCQPEVDAGQPVDLIYRIAQLTRMLRESMRELGLDQAIK
                     DAAQAIPDARDRLRYVASMTEQAANRVLNAAEKIQPIQDGLRRDAQALDTRWQAWFDH
                     PVELEQARTLVDDTRGLLRSIPDRAQASQDSLMEIILAQDFQDLTGQVIMKMLNVVGA
                     IETELVQVLLDNVPQDRRNDTASLLNGPQVSPGGRPDVVTNQDQVDDLLSSLGF"
     gene            39943..41103
                     /gene="flhB"
                     /locus_tag="VFR20_01395"
     CDS             39943..41103
                     /gene="flhB"
                     /locus_tag="VFR20_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HEU0229201.1"
                     /translation="MAEESDLEKTEPATPRRLEKAREEGQVVRSRELNTFLLLLAGVG
                     GLWAGAGDIYRSLYTAVHATLWIDPRIARDTGVMATRAIDAGLGVAWALLPIFALLVV
                     VALVAPMLLGGFLLSSKALAPKFERLNPVKGFVRLFSSQTLIELIKTLVKAVLVGTVG
                     AMVIWHYRDRMIGLMYAGSTEALAEGLNLVALCSILIVGSLALIVLIDAPWQLWSHFR
                     KLRMSREDVRQENKESEGDPHVKGRIRQQQRAMARRRMMAQVPNADVVVTNPTHYAVA
                     LAYSEGRGAAPRVVAKGSGPIAARIREIAAGNHVPVLSAPPLARALFHHVDLGREIPT
                     ELYAAVAQVLAWVYQLRRWNAGQGVQPVVPDGLIVPEGMDPGSAPDATAVVA"
     gene            41100..43217
                     /gene="flhA"
                     /locus_tag="VFR20_01400"
     CDS             41100..43217
                     /gene="flhA"
                     /locus_tag="VFR20_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEU0229202.1"
                     /translation="MNGLIALLKTNGAQNARLMAGPILILMVLAMMILPLPPFILDLL
                     FTFNISLAVMILLVAMFTKKPLDFAAFPSVLLFATLLRLALNVASTRVVLLNGHKGPA
                     AAGHVIEAFGHFLVGGNFAVGIVVFVILVIINFVVITKGAGRIAEVGARFTLDAMPGK
                     QMAIDADLNAGLIGEDEARRRRAEVGQEAEFYGSMDGASKFVRGDAVAGLLIMVINIL
                     GGLIVGVAQHGLSFSDAGRVYTLLTIGDGLVAQIPALVISTAAGVVVSRVATDQDVGQ
                     QMVGQLFTNPTVLFITSGILGAMGLIPNMPHVAFLSLAAALGGGGWVMRRQALLEHAR
                     QAQEPMRKQAAADAAAAEASWDDVSLVDPLGLEVGYRLIPLVDHAQNGELLHRIRSLR
                     KKFAQDVGFLAPVVHIRDNLDLKPNDYRILLSGVEVGRGTAMPGQWLAIDPGGASMTL
                     PATPTKDPAFGLPAFWIDVALREQAQIAGYTVVDAGTVVATHLNHLMHRHGADLLGRQ
                     EAQQWLDHVGREAPKLVEDLVPKTVSLTALQKLLRGLLDEEVAIRDARTLIEALAEHA
                     PRLAALNPTGAGPDAGELLAQVRVALGRAITQQWFAGEGDLCVIGLDARLENVLVQAI
                     GTSGALEPGLADTLLQGAAQAVQTQERDGHPTVLVVVPALRVALSRFLRHHLPQLGVL
                     SNVEIPDERILRVTAMVGRSAAT"
     gene            43214..45508
                     /gene="flhF"
                     /locus_tag="VFR20_01405"
     CDS             43214..45508
                     /gene="flhF"
                     /locus_tag="VFR20_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HEU0229203.1"
                     /translation="MNLSRFVGATSREALRQVRLALGPDALIVSNRRVNGGVEILASD
                     PTSATSEEPGIEPRPRPEAPTQGVMGAIESMRGALEARLDEAMWGNRLRQAPQAARLF
                     QSLLGLGFSTALLRAMLTRLPAGLGQGAAMRWVRDELARHLPVLAREGDLWKPGLVLA
                     LVGPTGVGKTTTVAKLAARCVRRTGPAGLVLVTTDTYRIGAHEQLKQYGDMLHVPVHV
                     VRNVDELRRLLGATHPSQTVLIDNVGVSQRDRYVAEQAALLAGGGRRVDRLVVLNASS
                     HGDTLDEVARTYTSDGGGALAGCLVTKIDEAPRIAAALDTAIRYRLAVHYVSDGQRVP
                     ENLRHRSPAELVDLALTRAGTGRDLFAPTEADLAALMEIASDDGPRIVGSREQRRMRL
                     LPGLLTGGDGRQPTLDALRRASDRLDDAPACAEAFGLWRAFIDEGATVDAAASSRHLS
                     RCAAGASVAPGTALAALHGRTRLDLPDGVPGELHATALFDGNMRSLTVPALQLALAGS
                     WQPVWGDPALTPPVAEAALLERLAWLDCNLQSNPLVHLLHGMSVTRARTLAEGGYAWL
                     AACAAKTMVMSDSCAAPIAALARELVFRPLLDPVRGASVSPDPLSSKSESTLWVAHAS
                     VHLSRRSATEPALGAVMVRAAEPGGRVGRIWCGLTNTEPSDVGLDQLGHWLVSLDQAK
                     QAFRHMGACWRLLAQSGSPSKPALHQALTAAQMGLAVWQAGKDEALSSVLRTLTPGRF
                     APHAALSRLFTFKEVLEREEGASA"
     gene            complement(45545..45985)
                     /locus_tag="VFR20_01410"
     CDS             complement(45545..45985)
                     /locus_tag="VFR20_01410"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HEU0229204.1"
                     /translation="MPPHTIDLRGCLEQECALVRHFIDVLHDEAQALEHPDDNTALQA
                     STHAKNQCAESLAQVSNQRDALLAQQGLPIGKQGLDASSRNDPALAPLCHELQALAAE
                     ARRLNLENGAAIEIYLTHTQRAIQTLRNLAGTSNLYNAKGRAIA"
     gene            complement(46011..46292)
                     /gene="flgM"
                     /locus_tag="VFR20_01415"
     CDS             complement(46011..46292)
                     /gene="flgM"
                     /locus_tag="VFR20_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HEU0229205.1"
                     /translation="MKIQTASSQTPLTGVQAANRPASTGAPAAGANNRSAVELSPAAR
                     QLSNLQNSDNDVDVERVNALRAAIASGQLTLDSGRVADKIIASARDLLK"
     gene            complement(46446..47144)
                     /gene="flgA"
                     /locus_tag="VFR20_01420"
     CDS             complement(46446..47144)
                     /gene="flgA"
                     /locus_tag="VFR20_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEU0229206.1"
                     /translation="MALLRTLPLLILFGLSGTASATMASTFQNPDAVARSVRHFLMQQ
                     ARAWPGTAQITVDPPDTSRLPSCDNPQVFLRGGQRLESRMSVGVRCLAPEPWTTFAQA
                     SVVIMGTYYVAGLPIPADATVVPGDLTPRQGDLLRLPAGTVTDPQDIVGRIAAYRIPV
                     GRPIRARALRSPESIQRGQFVRTVAQGVGFIATGSGKALENGPPGAQIQVRTSSGRII
                     SATVVDAHTVRVAM"
BASE COUNT         8514 a        13969 c        15591 g         9352 t
ORIGIN      
        1 ttggctcccc gagctgggct cgaaccagcg acctgcggat taacagtccg tcgctctacc
       61 gactgagcta tcggggaaca tccaaagaag cgaaactata gcatagaaaa aatgacgcgc
      121 gcaaacgcgc ttccgtggca aacccgggtg gtcaaggcct ctttatatgt tcgcttccgg
      181 ccggcgcccg cttgccaacg gtgcaggagg gaatggcctg caggaatgcc ttggtgggtg
      241 tcattgccgg acattggccc accaccagtc gcaggccgct tccacggtct gggcctgcac
      301 gctgtgcgcg aacacaaacg cttcgccgtg tagggtttcg tccgggaatt cggtgatcag
      361 cgtcacgccg ggtgtctggt tcggcgcctc gacagccgtg taaggcacgc cggcttctag
      421 caggaacggc atgtggccag aatggcgttc gtaacgccgc aactgttcct cgttgaatgc
      481 gcgcaggcgc gggtttccag ccagagactg cgcgacatgc cgcagcagcg cgaaggcggt
      541 gtcgcgccag gccgccttgt atcgcaggat gaggaagaag cctttgggca ggctccatat
      601 ttcaaagccg cgcggcaggt agccggtgca ggggcgtgtc cattcgtgcg ccgggtagcc
      661 gtgcaggttc aggtgcagct gcgcgccgct ggtttcgaag gcgtggttgc gtgcgaggcg
      721 ttcataccag ggggcgtgct cgcggtattc cacatcgtcg cccagagccg tgtagcgcgc
      781 ggcgtggtgc atgtgggtgg ggtgggtggc gcataaggcc tggtgcaagg cgtagccgtc
      841 cgggttctcg accggcatca gcacaaagtg cgcatcgggg cgggccgcga gtcgcccggt
      901 ggcgcgcagt gccccgacca cgcccgaggt ttcgttcgcg tgctgggcgg cgctgatcaa
      961 gacggcgggc agatggccgg gcacatgcac cacgttgacc gggcgtcctt ggctggatgc
     1021 gaaggtgatg tgccgtccct gataggcttg cagggctttc tcgatctggt tgaacggcaa
     1081 tggcgatacc gctgccggga gcgcgacggt tttcccgggg atgtcccggg tgtcgacagc
     1141 cggcaactgc tttggagccc gatgctgtgt tggcgtgggt gttgtcgtga cggtgacgcg
     1201 gatactctcc gcattattgt cgatcgtgat gtctggaacg atttgcccgg gttgcagccc
     1261 gcgattgccg ggtggacggc ccgagtattg ttggaagtat tcgagaatcg agaaatagag
     1321 ttcttcgtgc agcgcttcgg cgaggctgat ggtttcgtcg ccacagtcca gtctttgttc
     1381 ttcgcagggt agcctgacgg atatggtcag gcggtcgaag tagggttccg cacggcccca
     1441 gtcatggttg atgacagcgg ccatggcggc ctggtaggcc aatgagtagt cggcggccag
     1501 gggttcgctg cgtgtcacgt tgccattgat ggcgaccatc cacgccgagg gggtgtagaa
     1561 cggttgcccg agaaaatcgt tcccgacaac attcggggtg ggtatatgaa gcgtttcgct
     1621 tcgacccgag gcatacccca gcgtgacgtc gtaagcgagc ggcgtcacgg tgccggtgga
     1681 tccgttatcg gcggcgggca aatccgcatc ggccgtcgtg gtcgctggca caaggtcgat
     1741 tcgcgccatt gggaacaagt ccaccagggg atgggtttcc aacaggaatc ggttttccgg
     1801 gcaggccgtg tggcgtgggt agacgatgcg cgcggtcttc agtgtgcccc gatccacgtg
     1861 ttccaggaag tagtgcagca agggtttgta ggcgctgaag acgcgcgcgc ggatcccggc
     1921 ctgcgccagt tcggcttcca ttcggcgtcg tcggacgggg ccttcgaaaa gccacaagtc
     1981 cacgacgctg ccggcgcgtg gatcggcaag cagtcgttgt gtcagttggc gcgcggtgcg
     2041 ctcgaaggag cgttccacca atagaatttc ttccatccgg attcctgtct ctgtcgaggt
     2101 tggatcgctc cggcggctcg agggtggtta cttctcctcg tcagcatccc tcttgtcggc
     2161 gtattcgaat tcggatgcgc gaacaaatca aacggcgaac tagtgggcgc cggccgccga
     2221 tgccgaaccg ctgcctggac tctccggccg cgcgatccag atcagcgcga tcaataccac
     2281 gaacaatata gccgatgcgt agaagaaatc cgccgccgac atcgtgaacg cctgctcgtt
     2341 gatgatgccg ttgatcagtc ccgccgattg tgtgtgcgac aggccgtgat gcgccagcat
     2401 cccggtgatc gcatcgaacg ctggttcacc cggcttggcc atttcggtca gttgcgcgtg
     2461 atgcagcgtg gcgcgatttt cccagatcgt ggtggtgatc gatgtgccga atgcggcgca
     2521 cagcagacgc acgaagttgc tcagcccggc cgcggctgga aggcgatggt gatccagccc
     2581 cgacagcgtc agcgtcgtca gggggatgaa gaaggcagcc atcgcggcac cctggatgaa
     2641 ggtgggcacc atgatggtgt agatatcgac gtcggtgttg aatcgggacc gcatgtagct
     2701 gatgagcgcg aagatcagga acgagatggt cgagatatag cgcgagtcgt acttgctgag
     2761 cagtttcccg acgatgggcg agaggatgat ggccagaatg ccgaccggcg ccgaggcgat
     2821 gccggcgtcg gtggcggtgt aacccagtgt gttctgcatc cacagcggca gcaggacgtt
     2881 ggtgccgaag aacacgccat aggccacgga cagcgtgatg gtgccagagg tgaagttcag
     2941 ttccttgaac aatgtcaggt ccaccacggg gtgtttttcc gtgatctccc agatgacgaa
     3001 gaacacgaag gagatgacgg cgatgacggc cagtgtggtg atgaggccgc tggagaacca
     3061 gtccaggtcc ttgcctttgt ccaggagaat ctgcagcgca gcgacccaga ccaccagcag
     3121 ggacagcccg agcttgtcta tgggcagttt gcttttcacc gactcgcggc ctttatacag
     3181 ctgccatgac gcccagcccg cgaccagccc gatgggcacg ttgatgtaga aaatccaggg
     3241 ccagttgtag ttgtcggaga tccagccacc caataacggc cccgccaccg gcccgaccag
     3301 gacggtcatg gaccatagcg ccagtgccat gccagccttg cttctcgggt aggacgcgag
     3361 catcaacgat tgcgacagcg ggatcatcgg acctgacacc gcgccctgta tgacgcggaa
     3421 catgaccagg gaccccagcg accacgaaaa cccgcacaac cacgaggcca gcacgaacaa
     3481 cagtgtcgac agcacgaaca gacgcacttg ccccaggcgc tgggtgagcc atcccgtgag
     3541 cggaacggtg atggcgtttg cgaccgcgaa cgacgtgatg acccaggtgc cctgggtggc
     3601 gctgacacca agatcgccgg cgatggtcgg tatggacacg ttggcgatcg aggaatcgag
     3661 cacattcatg aacaccgcgg cggacagggc gatggtgccg aagaggcgcg ccttgccttc
     3721 cagcggaggg tatgaaacgt gcttgggtgc gacagccttg gctttgctgc ttgtttctga
     3781 agtcatgtac gggaaataac gaggtacggg ttttgcagtt gatcagcgga tgccatggcg
     3841 tgaggtccag gccgcgccgt gccctccagc agagggaaga gggcacggtg ggcgggtgac
     3901 gacgccacca cggcgtcgtc acccgctttc agagtcatga gcccaggttt gcttcgatga
     3961 tcttgttgat gagggcattg gcgccgatcg cgttggcctg gaaggcattg gtttccagtt
     4021 tcgtgtcggc ctgggcgtca tccggctttt tcgcagggct ggccagatcg acttcaaccg
     4081 ccatcgacag gccgacacgc agtgggtgtt tgacgaggtc tttggggtcg atggtgatcc
     4141 gcaccggcac gcgttgtaca accttgatcc agttgcctga ggcgttctgc gccggcagca
     4201 gggcgaacgc gctgcccgtg cccgccgcga tacccaccaa cgtgccgtgg tagaccacgt
     4261 cactgccata tacgtcggac gtcatggtga cggactggcc tggcttcatg cgtgcgagtt
     4321 ggccttcctt gaagttggcc tcgacccaca cttgcttgag cggcacgatg gtcatgagcg
     4381 gtgtgccggg ggcgacgcgc tggccgacct gggccgagcg cttggccacc atgccaccga
     4441 ctggcgcggg caggacggtg cgcgcgttgg ccagccaggc gttgcgaacg cggtcggcgg
     4501 cttgccgcac atcggggtgt ttcgccacag tggtccccga ggtcagggcc tgattggtct
     4561 cgagtttcgc gcgggcgacc gcgagcgcgg cgtgggcctg tgccaagctg gattcggcgg
     4621 ctttctcggc ggtttcggca tgcaggattt cttcaccgct gacgccgccc gacttcgcca
     4681 gcatgcgccg acgcgccaga tcgctcttgg ccttggcgag gtcgaccttg gcgcgttcga
     4741 tatcagcctg gcgcacggcg atgtctgatt tcagcgcgtc gttctgcacg aacagcgtgc
     4801 gtgtatggcg tacggcctgg gccagggccg cttcggcttg cttgagcgcc acttccgtgt
     4861 cggtcggatc gagcttgatc aggggttgcc ccttgtcgac gtgttccgtg tcgtccgcat
     4921 cgatgcccac caccgtgcca gcgatctgtg aagtgacctg caccaggttg ccgtggacgt
     4981 aagcgtcgtc ggtggtctcg tagtgtgagg caaccaccac ccaccagatg gcatagcaga
     5041 cgccgatgac gatgaaaatc agagcggcgg tcaatagtag gcgtttgcgt ttcggtgccg
     5101 atttgtgttc ggtagtcatg taagtctcgt tgggtgatca gtgtgattca acagttgtcg
     5161 tggtcgcggc agttgccccg ccgttttgcg tggtcatggt tttgggagtc tcggcggggc
     5221 cgtagccgcc gcccaacgcg gtggcgagtt gcgcatccag gttataggcg cgcgcgcgca
     5281 accgggtgtc ctggatggcc tgtttgagga cgtcgttctg cgcgagcagc acctgcagga
     5341 aattccccag accggactgg tagcgcttga cggcaatctc gtaggccgac ttgatcgact
     5401 cgtaactggc gtgctggtcg atcagttgtt gccgcaggtc gcggatggca gtggtggcat
     5461 cggccacctg ccgcacagcc gtcagcacag tctggttgta gttggcgatg gccaggtcgc
     5521 gttcagcccg tttggtgcca agttgggcgt tgagggcacc gccgttgaaa atgggcaggc
     5581 tgatggccgg acccacaccg tacgtcttgc tggaatcatg cagcaggttg cccagaccta
     5641 gtgcctgaaa gccgaagaac gctgacaggt tgatgtcagg gtagaaccca gccttggcgg
     5701 cggaaatgtt actggacgcg gcttcgacac gcagcttggc ggcgatgatg tccggccggc
     5761 gccccagcag attcatgggg atgatcttgg gtatgcccgt gggggcgtgc gccagtttgg
     5821 cgcgctggat cgtcatgccg cgatcggggc ccgcgccgac cagcgcggcg agctgatggc
     5881 gcagcaatga ggcattggtg ctggcctggc tgagctgaac cttggccgcc gacaccgccg
     5941 aatccgcctg gttgacttcg acgcgggtgt ccagtccggc cttgagccgc tggcgcgtga
     6001 tgctggccac gttttccagt tgtttgacga tcttgccgat gacttcctgt tgttccaatg
     6061 cgtcctgcag gttgaaatag ctctgcgtga ccgcgctgat cagcgcgttg cgcgccacct
     6121 cggtgtcggc gcgcgaggct tcatcacgtg acagggctgc cgcgtacaac gagcgttgct
     6181 tgccccagag atcgaggtcg aaccccagat tcagttgcag attcacgtcg gtttgcatcg
     6241 agccaccgag ggggggcgga tagatgtagt tttcggagag ccgctcgcgg gtttggttat
     6301 aggtggcgtt gagattcggc aactgcacgg cgcgtgcgcc atggaccgcc tcgttggcga
     6361 tccgcaacct ggcctgcgcc gcggcgagtg aggggttttt gttcagcgct tcggagacca
     6421 gcgtgtcgag ttgcgggtcc tggtagcgat gccaccactg gcgcgcgggc catttgatgg
     6481 atgcgcccga caggcccaac tgtgtcccct ggatctcttt gacggccggg ttgcctggat
     6541 ccatggctgc gcagccagtg agcgtgaggg ctagaagaat tgggatgagt ggtttcattc
     6601 tgacaggaaa ctgaatttat ttgataagta cgtatctgac taggcaatta ttgtatatca
     6661 gccgtgcggc gttatgagtc tctggcacga tatgttcacc gattggtgtg tcggctggtt
     6721 gacgatcctg atgttcagga ctggtcggga aaatcgggtt cggattcagt ggagccgttg
     6781 tcgatcaatc gacccagcag gttgagcagc gtttcgattt ccgcgtcggt gaaaccggcg
     6841 aggtggttgt tgaaggcttt ggccacggcg ggaagtatgt cgttgacgac gtcgcgaccg
     6901 gcctcggtca gctcaagatt gacgacccgc cggtcggtgt cgcaacgttg acgcctcagg
     6961 aagcccttgg cctcgaggcg gtcgagcgtg cgcgtcatgg caccggtgtc gaccgtggcc
     7021 ctgcgagcga gttcggccgg ggtgttggtg ttctggtggc agatgatgac cagcggcttc
     7081 cactgcatgg ccgtcaggcc catgggcgca acgtatccgt cgatcatgcg gttcagcgac
     7141 gtgtacgatt gcttgagcag ccggcccagg ttgtttttgc tgcgcagcga cgcgacatcg
     7201 gcccggtaga aggcggctgg tgtcttggcc gcgacgttct ggtcggatcc cgacgtgggg
     7261 gcgctggcag gggaggcgtc tggcgcgctg gctgatggcc gggccggacg tgcccgagag
     7321 gaattgggcg tgttctttgg catacccgaa ttctacgaca caataactgc ctagacaact
     7381 attgttcgtg cattatttaa ctcggcaatg acctcgcagg cgcggcttgc ggaaactgtg
     7441 ttgctgtttc acaacgactc ccgggcagga tctgttcagt tgctttgaat actcaatgtc
     7501 cgctccgcgc tcgccgcgcg tccggattgc ggggaaatcc gggttcaggg aatcagccct
     7561 ccgattccgc ctcgatgtcg tgccgatgca cgtcgtgggt gatgaatccg gatacgttgt
     7621 cgggcatccg caaatactcg ggcttggcca gcgtggcccg gatatcggcc agaccgctgg
     7681 cccagtgatc gcgcatggtg gccagcccga actggtagtc cttgtagtac tgctcgtacg
     7741 gtttgtcccg gtatatcaaa tgaacgacgt tgtaccgctt gaggcaggac atctcttcgg
     7801 ccaagtgggc gtagcggtcc tgtgcgcgtt ggtcgggagg gatcttgtcc agcaaatacg
     7861 ccagcatgtg ccgcaggtgt tgcaaccggt gcatgtcttc ggtgatcagc cgcgtgcggc
     7921 tggagtactg gatttccttg acccggtcgg agacttcgct catgttggcc ggcgcccggc
     7981 cgcgcgcgct ccataggtcc acctggaatg ccagggtgtc tttctgagga cgggattcca
     8041 gcacgtagga cagcggcgtg ttcgagacca ggccgccatc ccagtagtac tcgccatcga
     8101 tttcgatggc ggggaaggcc ggtggcaggg cgcccgatgc catgaagtgg cggggctcca
     8161 ggcgcgttct ggcattgtcg aaatacacga agttgcccgt acggacattc acagcaccga
     8221 cggacacgcg atgggtgccg gcgttgatca gatcgaaatc gcacaggcgt tccagcgtat
     8281 cgtgcagcgc gtgggtgtcg tagtagctgg cggtgcccgg gtcgcctggg gtcgtcatgg
     8341 ccggtggcgg catgcgtggc cggaaaaacc cccgctgacc gcccagcacg gcgcctgcag
     8401 cgtgcagcgc gctcatgttg cggcgcacgg tgtcattcat gttgaagagg ctttgttccc
     8461 agaacggatt catggccagg ccaaggttcg gctgacagat ggtttcccag aattccgtca
     8521 ggcgggccac ccgtttttca ggtgggttgc cggcgatgag ggcagtgttc agcgcgccaa
     8581 tcgaaatgcc ggaaagacaa tcgggttcga tgccggcttc ggctagcccc tggtagaccc
     8641 ccgcctggta ggcgcccagc gcaccaccgc cttgcagcac gagcgccacg gtttcgtaag
     8701 ggggcagggc gggccggacg gtgtcgtgga cggcgggcgt ctttcggggg gtcgtcatga
     8761 gtcgctccag gtcgtgaggt tgggttccag gctgcgcacg aaggcctcga tgagcgcgcc
     8821 gcggtagcgc gctttgtgca gaatcaggga caagcggcgc tgcagggcca ggaaaggtgt
     8881 gtccagaact gccaggcggc ccgtggcgac ggcgtccacc gtcgccgcgc cgggcaggca
     8941 ggcgatgccc agtccggcga tgacagcttg tttgatggct tcgacctgcc cgagttccaa
     9001 cagaatgcgt cccgggggca ggctggccat gagggtttcg gattgtgtgc gcatggccga
     9061 acccggctct cgcaggatcc aggcggcctc cgcgaaatcc gagggacgca ggcgccgccg
     9121 gcgaaccagc gggtgatcgg gcgccgcaca cacttgcaag tcgtcgttgc gccacggcag
     9181 cgtgtcgatc tcgggatggt tcacggggcc ttccacgcaa cccacatcga tacggtgttc
     9241 cagaacgccc gcgacggtgg tgcgcgtgtt ttcgacactg acgctgagcg acacgccggg
     9301 gtggcgccgc acgaacgcgc ccagcaactc gcccaccaga tagttgccaa tggtgttgct
     9361 ggcgcccacg cgcagttcgc ccgatagggc gtgcgtgtgt tcgtcgggct cgcgcagcat
     9421 ctcgtgcatg cgctccacga cctcctgggc ggcgggcaag agttctttgc cgcgtgtgct
     9481 caggtgcagc ctgccgcgga tgcgatcgaa cagcggcgcg ccgatttgac gttcgagttc
     9541 cgacagagcc atgctgaccg cgggctgcgt gactgacagg cgagtggcgg cggcgcgtac
     9601 gctgcctgcg ctggccacag ccacgaaaac ctcgagttgg cgtatgctga tcccgatcat
     9661 cagtggattt tatatgtaat tggtatcggg ataacctcat gcgatctgtt cagaacgtgc
     9721 tgttcatcat ggccgaccag ttgcgcgcag accatctggc ctgttatggg cacccacgca
     9781 tccgcacgcc gcacatcgat gcgctggccc ggcgcggcgt gcgtttcgag cgggctttcg
     9841 tcaattccgg tgtatgcggt ccgtcgcgca tgagctatta caccgggcgc tatccctcca
     9901 cgcatggcgc aacctggaat cgcgtaccgt tgtcggtggg cgagctcaca ctgggcgatt
     9961 acctgcggga acaccagttg actctcgccc tggcggggaa gacccacgtc atgccggaca
    10021 acgccggcct cgcgcgcctg catctggatg ggggctccga gcttgggcgc ctgttgcgcg
    10081 ccggcggctt cgaggaagtc gatcgctacg acgggcacag caagcctggg gcggaaagcg
    10141 gctatcccgc ctatttgcgt cggcacggct acgcgtccga cgacccctgg tcggacttcg
    10201 tgatctccgc gcgggatgcc tcgggccagg tcgtcagtgg ctggcacatg cgcaacgcgg
    10261 ggttgccggc gctggttgac gagcgccatt ccgaaacggc ctacatgacg gatcaggcgc
    10321 tggcgttcat gaagaagaag ggcgacgaac catgggtgct gcacctgagc tatgtgaagc
    10381 cgcattggcc ctacctggcg cctgcgccgt accacgcact ttatggtgtc gatgattgcc
    10441 tgccggtggt gcgcaacgag gccgagaagc aggatgcaca cccggttgtc gcggcgtatc
    10501 gacagcaaga ggaaagccaa agcttcacgc tggacgaatg catccgggcg gtgcgcccga
    10561 cctatcaggg gttgatcacc cagctcgacg atcacctggg gcggttgttc gactatatgg
    10621 agggggccgg cctcatgcgc aacacgctgg tcatcctgtg ctccgaccac ggggatttcc
    10681 tgggagatca ttggttgggt gagaaggagc tgttctacga cgtggtgcag cgcgtgccgc
    10741 tcatcgtggt cgatccgtcc gccgccgccg acgccacgcg ggggcgggcg gagtcccgct
    10801 tggtcgagtc ggtggacatc gtgcccaccg tgctcgaatc cctgggtatt ccgttgccgt
    10861 ggcatcggct ggaaggccgc agcctgtcgc cgctgttgca tgaccaatcc gtggcgtggc
    10921 gcgattgtgt gttctccgaa ctggattaca gctatcgcct ggcgcgggtg ctcacgggca
    10981 agacaccctt caacgcgcgg gggtggtccg cgcgcaccga ccgctggctg tacgtctatt
    11041 ggctggatga gccggagcag ctctacgacc tgcgcgacga tcccgatcag ttccatgatc
    11101 tggggcgtga cccggatcac gaagaggccc gccgcgccat gaaggcgcgc ctcttcgact
    11161 ggttcgcggg cttgaagcac cgggccacgg tcacggctga atcggtcgag aagggcacca
    11221 acgcttacaa gaaggccggt gtgttctacg ggcagtggta gcgcggccat cgccagggct
    11281 tgcgccttgc ctgacatcga tcccggctgg tttattgtcc gaagaacttc cagttcagga
    11341 cgctggtgtt tgtcgggcgc tcaggtgagc cacgacatcg gattgtgcgc cagcgccacc
    11401 cccacgatgt aaaagaaaat ggcgatggcg atcagggccg ccacggcgcg ggtcgaggcg
    11461 gtcttgccgc gcttgatggc aacggtgccc accccgatgt aaaacaccaa cgcgatgatc
    11521 ttggccacta tccaggtttg caacccgccc agcatcactg tgaggatgat gccgcacacc
    11581 agcaggacgg tatcgatgac gtgcggcagc actttcacga tgcgtagctg cagccggctc
    11641 gaacctgtga ccgaccacca cgcccgcacc acgaaaaaga tgatgctcag ggtggcggcg
    11701 ctgatgtgca ggtgcttgac gagttggaag tacatgacga tttgtggtta tcccgttgac
    11761 gttgggtgcc caaggccggg gcgggcggat tccattaccg tggaatccat cgatcctact
    11821 gcaggaccca ccgaccctct ccctgaagaa tgagctggcg cacatggtgc ggcaccaggg
    11881 tgctgcgatg gccaacactg accaggatgg tgttgggcag tagttcgcgc acgagccggt
    11941 acatggcgtc ttccaacccc tcatccatgg ccgacgtggc ctcgtccaga aacgccgcca
    12001 gcggcgcggc gaggagcagg cgaccgaacg ccaaccgctg ctgttccccc agcgacagaa
    12061 tgcggcccca gtcggccact tcgtccagtc ggtcggtcaa gtgcccgaga tggattcgag
    12121 tgaggatctc ggcggccttg ccttgggtat cgacgggggg cgggtcattg attggaacca
    12181 gcgcgttcgt tgccgtcggg gatgacaggg cgtcggccgt gcgaaatgaa acctccggca
    12241 gcgcgtggct ctggcggtcc agcgtcttgc gcgtgaaccc ggcgcgggcc agatcctgct
    12301 cgagattgcc ctccgaagcg ggggttgcca tgccgtcatg caagacttgg ttggggtagt
    12361 acagcgcgtc gcgcagactg cccaatggca gatagggctt ctgcgaaagg aaaagcgcac
    12421 tgtgctctgg ccggatgata tcgcctgagc agtagggcca aaggccggcc accgcgcgca
    12481 gcagggtggt cttgccagca cctgatggtc cgcggatcag cagtggcgtg ttggcgcgcg
    12541 cctcgagggt gagccgctcg agcagcacgc gacccgccgg ggtcgtgatg gtcaggtcgc
    12601 gcagggcgat cagggcgcct tcctcacgca catcgggttc gggcagcgaa tacgattcga
    12661 tgatggccga cgagaagccg gtcagacggc tcagggtggc gcgatacgcc gcgaaactgt
    12721 catacgcgtt gcggaagaat gacaggttgc tctgcaattg cccgaaggcc tgggcggtct
    12781 ggatcaggtc tcccagtgtg atctgcttgg tgaagaagcg ctgggcctgc aggatgaacg
    12841 ggaacaccac ggcggtttgc gagatggcga agttgaatcc caggaatttc aacgaccggt
    12901 acacgatggc ccatgcgttg ctgatgacct gtccgaaccg gcttctcaac agcgcgcctt
    12961 caacgcgctc gcccgcatag aaggcgatgc tttccgcgta ttcgcgcatg cgcaccaaag
    13021 cgtaacgata gtcggcgttg aagcgctcgt tcaggaagtt cagcagaatc agcgggcggc
    13081 caatgcgtat ggcgaaataa gtggcaatga tgacgtacac gaacaccagg aacaccatgg
    13141 ctcgcggaat gctcactccg aacagattca gcggcccgga cagattccac aggatgaggg
    13201 tgaaggcaaa ggtggatacc agtgcgttga ccagacccat ggacaggctg agggacgttt
    13261 gcacaaagcc ggccacgtcc tgttggatac gctggtcggg gttctcgact ggggtgtcca
    13321 ggtactgcgt gcggaagtag gcgcgcttct cgagccaccg gctgagcagt ctttcgttga
    13381 gccagacgcg ccagtggatg gtgaacgcct gctgcacata gaattcgatc agcgaacgta
    13441 tgacgtgcgt ggtggccagt atgccgaaca ccagcatggc gaaccagaag cccgcttcgt
    13501 cgagcttctg cagcgagctg tacatgctgt tgtaccagtt ggagaacagc acatcgaggc
    13561 gcaccgcgcc cagggtcagc agcagtatga cggcgaccac cgcaacgggc cgccagttgc
    13621 gtcgcgacgt gaaatacgcc ccggaaatat cccagaactg gcgcccccag acggtgaagc
    13681 gggccaccgc ccagaccgcc acgcagaaca ggatcgcggt gatgatgtag gctttcagca
    13741 gccagatggc gctgtcgatc aactggtggt tccagtccat ggagtctccg gtgtgagcgc
    13801 ggcacgcgcc gtcgagtggc attctggatg aaaagaccgg tttgttcacc cagggttccc
    13861 tgggaacgcc atgaatccga gggtttgttc agggcgtccc ttgcgcattg tacgggggtg
    13921 cgggcgggtt gtcggcgttt gcctgtcccg ccgccccggg gccataatga cagctccgtc
    13981 tgaaactcat ctggaactcg aaaacatgcc ttacgtcacc atcacgtcca gccgcgggct
    14041 gtccacggag cagaaaaaaa acctgttgca gcattccagc gacgcggtcg tgcagagcat
    14101 cggcgcgccg ttacccagtg ttcgcgtcat gctgcacgag ctgcctgacg gacactatct
    14161 caacgggggc agtttcgaca cgcgcgcgtt gatgttcgag gtcgatttca tcatcggccg
    14221 caccgacgac cagaaggcgg cgctcgtcgc ggcgctcagc cacgcggggt ccaacgtcac
    14281 cggcgtcccg gaaaatgaag tgcgggtccg gttggtcgat tttcccaaga ccaacatggg
    14341 catggcgggt ggcgtgaccg ccgatcgcct ggggcgttaa cgccgggccc atgcgggggc
    14401 gggtttgcct gacttttagg ggccagtccg gtggtggtat gctgaaggca cgaacaccat
    14461 ctggataagc cgccctcgcc ccccggggca ccgacggcat gtgaatgtgc catgactcaa
    14521 gacgcccaac ctttgcggtg ggtggcaccg gcaagtgatc cggccgatgc caatgtctcg
    14581 aagccgggta gcctgagcga agcgcgcgcg gtagtggatt tctggcgcga cgccggccca
    14641 aaagcctggt tctcgaaaga ccctttgttc gatcaagcgt tccgcagccg gttctccgag
    14701 ctgcatttcg cggcggcgcg tcgcgaacgc gaccactggc tcagcgaccc gtacgcctgc
    14761 ttgagcctca tcctgctgct cgatcagttc ccgcgcaacg cgttccgcgg cacggggcac
    14821 atgtacgcca ccgattcact ggcgcgttgt tatgcgcgcg ccgccgtgaa tttcaagcat
    14881 gtgaagcatc tggaagacgt gctcaggccg ttcgtgtttc tgccattcat gcattccgaa
    14941 agcctcgagg atcagcgtta ttgtgtggat ctgtaccgcg attacgcgcc gcagaacctg
    15001 acgcatgcgc aggaacattg caacatcatc cagcgttttg ggcgctttcc ccatcgcaat
    15061 gtgcaattgg gtcgcgccac aacacgggaa gagcagcgat tcctggataa tggcgggttc
    15121 tctggctgat ttcccttcgc tggcggcgaa cgccgggacg gggactctgt gtggtcgatt
    15181 tggcggcttc atgtgtccgg gatatcgtgg agcctcgttt tgtttcagcc cgcggcggcc
    15241 aagcgcccgg tcggacgggt gagggcggcg agcgtgaatg tgagcgccag agtcggcaac
    15301 gcgctgcccg cggcgggcgc ccattgcgcc agcccgtggt agacggcgat ccccagtagc
    15361 cagagggccg cggccacctt gtcgatcttg ggtccgtgcg tggtcaggct gggttgtgtc
    15421 aggcccaacc gccccagaat gacaccataa agcggaacga agacggagct tagcaaaagc
    15481 aggaaaggct ccaggctgtg catggggagc accagggcca gcagagtgct ggcgagggcc
    15541 agggtcagcc cccagcgccg gatgctccaa cgcggttcga tgctgtgcgc gcacacagat
    15601 cccgaataca cgtcgccata ggcgttgtcc agttcatcca ccaggatcaa ccccaacgcc
    15661 accagaccgc cctgggccag cagcagggcg ctgaccagat ccgtgccggg ttgggcgacg
    15721 ctgaccacca gcatgcccag cgcatagcac caggcgttgg ccagtgtata acccagccaa
    15781 gtgccgccca gcgcgccgcg cccgcttttc ccgtgccgcg catagtccgc cacgagcggc
    15841 agccacgaca caggcatggc gatgaccagg tccagcgccg aaaacatgcc catggtgcca
    15901 tcgccccggc ggttccagat cgtggccagg ctgtccgcgt ggagtttccc ggcgaactgc
    15961 cagctcaacc acaacaatga gaggaccacg agcggaagcg cggcgcggct gatgatggcg
    16021 cggaccagcg tgatcatcga acccgcgagc agtgcggtca ggaccaggcc ccagaacacc
    16081 gtggccgcga cgtacgcggc cacgccacca aagcccggcc ccagcgcctt gacgaggata
    16141 ttgaccgtgc cttcgcgcat gatgaccagc tcgaatgtcg tccagccaag caactgcacg
    16201 atgttcagca gcacgggcaa gcgcgagaac gaatggccgt aggttgcgtg gatgaggccc
    16261 gcgctggaca ggccgctttc acagcccgcg cgggcgaccc aggccagcag accggagccg
    16321 atgagggacc cggcgatgat ggcgaacaag gcttccctgg tacccgcggc aggcaccagg
    16381 taggccccca cctgcatgac cagcaagccc acccccaggc tgaaccacaa ggaggcgtgc
    16441 tgcgtccagc cgaacacccg gtgcgtgccg tccagcgggg taagggcctg gtttgagggg
    16501 gactcggtcg tgcttgtcat gatgtcttgc ccggtggcgg cagggacgac aggcacagaa
    16561 caggcgcgta gcgctgattt gaacgattgc cgctttcctg cgcgaggatg atctcagtca
    16621 ggttcaaagg gactttctca gccgcgtggt gatgcggcac ccctagcgat gtggtgatgc
    16681 gtggcgttga ttgtagagca gcccgcgcgg agcttcaatg tgtttgcgtc gggcgtgggg
    16741 cgtctgcgtc aaggcgtttg cgtcgaagcg gggggagaag ccgccgcgat gtgctgcgcc
    16801 aacccattgg tgaggtcagt catgaagaca ttgctcgcat cggcggcgat acccaggatg
    16861 gcgtgcagcg acgagatcgt gtcgggatcg tagtgcttgt catggcccga tgcgcgctcg
    16921 aagccattgg cgatgcgcgt gacggcgtct gtgtccacag cggccagcag cagcgcgtgc
    16981 gccagcacga cctgtaccgc gtccgtccgg cccggagcat cccggatcgt cggggtgtcg
    17041 ccttccgggg cgacgcggcg ccaaagctgg gcggttcccg cgatcttgcg cgcggtgccc
    17101 gaatcgtcca gccaggcgag gttgtagcgc ccgtcacaga acgagccggt gacggcctgc
    17161 ggccgggtgg cgatgcccaa gtcattcagt gcgcccgaga tcagcgcgca caggctttga
    17221 taagcggcat cggactgcga gagcggctgt ccggccgcca tgtaggccag gctgatattg
    17281 aggatgcccg gcccttgggg aaccagccct ccacccgact ggcgcacggt cacaggccac
    17341 cccagccgcg cgaagcgtcg gcatgcctcg gcaaatcggg gaaggcggtg gtaagtgcgc
    17401 ggcacgacca acccctgccc ggttcgccag atgcaggcca ccgggctgcc ctgggccgcc
    17461 cgccgcatca ggaattcgtc gaaacgggcg tgttgggcct ggcccggttc ggagggtagc
    17521 agggtgaacg acatgccgcc ctacacctac atcacggccg atccggatgc cgtgaagtcg
    17581 ctgtccgaca tgaacaggta gcgcaccgcg tcgatcaggc tgagcaccac gaccagcgga
    17641 gtgaagacgt acagaatcac caggtacagg atgcccatgc ccacctggcc gagatagaag
    17701 cggtggcctc ccagccagcc caggaaaatc gccaggacga tggccatttt ccgccggctg
    17761 cccggtttgt cgagctcgct gcctttgttc ttcatgatcc accaggtcac cgggaccgtc
    17821 agcaccagcg ccgccaccac gcgtcctgtg tggccgtcga tatagcccgc gacggcccgg
    17881 tagaggtcgg cgaagttgta gatgaccaga ccggtgatgt gggaaatcca ggcgaacaga
    17941 tcgtggctca gggattcgcc gaaggtcagg gcgatgagga tgatgacgaa gatgacgatc
    18001 ggcaggacgg ctttctgcag catggggcgg gcctcgggtc cgtggacatg tggccagttt
    18061 acccgcgttg ttgggtgctg gggccgccct ccgggtgggt agtggccggc gaatggatga
    18121 tcaagggtgc gccacggtca atcgccacaa ggtggtgact tcatcacgtc gcgcggcatg
    18181 cagtggatca ctggtgtcaa ggaccttggg gtgccgtggc cgggtgtcga gtcgtcccac
    18241 gacgcgcagg cccgcgcgcc cgatggctgt cgccagaaag tctggtgcgc gcaggcccag
    18301 ccgctcagcc agatcggaca gaatgagcca tccttcgcct tctggcagca aatggtccgc
    18361 gaggccatcc aggaagcccc gcagcatccg gctgccggga tcatagatgg cggactccag
    18421 cgaggtggtt gggcgggccg gcagccaggg tgggttgcag acaatcagcc cgcaggcacc
    18481 agaagggaac agatccgcct cttgtaccgt cacgcggccg gtcaacccct gtgtctcgac
    18541 gttctcgcgt gcgcaggcca gggcgcgcgg atcggaatcg gtcgcgacga cccgcgccac
    18601 gccacgccgc gcgaggatag cggccaggac gccgctaccg gtgccgatgt cgaacgccag
    18661 gtcggttgcc ggcagcggcg tctgggcgat cagatccaga tactcccccc ggatgggcga
    18721 gaacacgcca tagtgcacgt gaatgggctc atccagaaca ggaacggcca cccctttgcg
    18781 ccgccattcg tgggcgccca ccatgccttg cacggcgcgc aatggcagca cgaagggctc
    18841 gcccacatcg cccagcgcgt cgcggcacgc cgcccgcacg tctggcgcat gccggagagc
    18901 cacggcccca tcgccctcca tgggaatcag aagctggttg agctgtgtgg cccgctgtga
    18961 ttgggcgagg cggtgctgat tgaaggcgtc gtgtggcgaa tccggctgct ttcgcttccg
    19021 tggccggtgg tcgacgcggt gggcaagcgc gacaagcaac tgtcgggcgt tgcggtaatc
    19081 gccccgccaa agcatggcgg ttccctggct ggcaagccgg aacgcttgat cggccgtcag
    19141 cgtatcgtcg acgacgcgga cgtgatcggg gtgtggcggg tcgccgggca cgcaccacag
    19201 ggcttgccgg gtttcattgc cttcctgcca ggaaatgtat ggctggggag ggggattggt
    19261 tcggggagtc atggtttgag agccctgcta catggttgca atgtgggatt tcaggacatc
    19321 cgtgaaatgt cttgtcagag cggagggggc tgtttgtgcg ggcatcgcca tctggacatg
    19381 aacgggaatg cgaggcgtga tgtcccgtac cagcagttgg cctgagaacg tgttggccac
    19441 atagggattg acgatcccga tgccgttgcc ggccgccacc aatgcgcaca cggtgatgga
    19501 tgagggtgtc tcgatgacga aatcgtccgc tatgccgtgt tcggaaagaa gggatcttgc
    19561 cttgatggtc agttcgtcgg tatcgctcaa ggtgagtatt cgttcccccg ccatatctcc
    19621 gatgtcaatg atggacctct ttcgcagccg gtggttcatg ggcagaacac acacggcgct
    19681 cgaggtcctg atggtggatg gccggaagtc attctgatcc agggtacccg tggtgagcac
    19741 cagatcgacc agatcctgat gcagatagtc agcgagttgc agtgtgctca gggtctggat
    19801 agtgatgtac gtgcgggggt attgcgacag gaacttgttg atgacttggg gtagcaggcc
    19861 tatcgccagg gtgggtgtgc agcccacgcg aaggaccccg gagccggatt tgcgcatgat
    19921 cgccaccacg gattcgattt tctccaggcc gtcgaagtgc ttctgtatgg tcttgtatag
    19981 caacttgcct tcgctcgtgg ggcgcagtct gccatggtgc agatcgaaga gcttgagttc
    20041 cgcggccgcc tgcaactcgg caatgcggcg actgatggat ggctgtgtgg tgttgagcat
    20101 cgcggcggcc cgggtcgtcg tgcccatttg catcacggcc ctgaacgcct ggatctgttg
    20161 gaagttgagt gatctggaca tgactgatgt gtgatgcttg tgatgaactg gcttgccccg
    20221 acggtagtgc ccggtgtgct gttgacgggt ggtggctatc tcgaagacaa gccggtggca
    20281 ccaagcttgg tggtgttgta atgtatatca aaattgaata gagcactcaa aaaatagaat
    20341 ttgatcgtat tgctgttgcg gcctactgtg tgcgtatccc atacagcagg ctgccacctt
    20401 catgaatccg cggaacattg tcgacaaacc ctgcccttgc cgccgactcg gtgagaacgc
    20461 cggcgctcat cacttttttg gctattacaa caagacggtc tgggaccgca ccggccgtta
    20521 cgtgctggcg aaccggaccg cgttcatgga tgccgatctg acgccggatc tgtctgccgg
    20581 aatcggtttt ttcgatccat tgaatggaga tgccttcttg gaggtcgaca gcacaaaagc
    20641 ctggaactgg cagatgggct gccagttgca gtggctggat ggccggccgc aacgccagat
    20701 catctacaac attcgcacag atgcccagac cgggccttat ccggggttcg ggtccagaat
    20761 ccacaacgtg gatacgaatg aggttcaaga tctgccgttg ccgatctatg tcgtctcgcc
    20821 cgacagccgc catgccttgt gtgtggatta cagccgcttg tacgttaccc atgaaaccat
    20881 cggctatccg cctcggccga gcgttctgcc caatgccccc gatgacgacg gtatccggcg
    20941 tctggatctt gccacagggc gccatgagct gatattgagc tacggtgatc tgtttcgttt
    21001 tcatcatcgg ccatcgatgg acaaggcgct ccattgggtg agtcacattg aaatcaatcc
    21061 cgattcaagc cgagtggttc tgctgcatcg gtggactgag cgcgtcgaag atgaaacctg
    21121 cttcctgcat cgcctgatca ccatggacct ggatgggtcg aacctccgcc tgctggagtg
    21181 ctccgatcat cctttgcccc agttggcgga tgatttcgat ccccacgccg tgggcacctt
    21241 tgattacgag aagtcgccgt atcagatatc ccaccccgcc tggcgggaca atgatcatat
    21301 cgtcgtgtgg agtcctcaca agggggagat ccactatcac ctctatcatg accgcgatga
    21361 cggggacgtt tgtgtcctaa gccccgggat cctgacggaa aatggccata tgacgttctc
    21421 gcccaccgac aaacggtggc tgctgagcga cacgtatccg gacgcggtga ccaacgagcg
    21481 cattctgttc ctgttgaatg tcgacgataa cgtccggtac gacattggcc gcttctacgc
    21541 cgacccgaac ctcaggaagg agaaccgctg cgatctgcac ccgcgctgga gccgggacgg
    21601 gcggtcggta tgcgtggatt cggtgcatga gtcggagcgc cagatgtatt tgctcgacgt
    21661 atcgacaatc gttggttgag cggagcggga accggatgcg ttatgctgcg catccgtgtt
    21721 tccaatcaca acactgattc actgatcagg tactacaagg agacacgaca atgaaggaag
    21781 caatagccat gataaattcc acctcacgcc agtgcgggcg ccgccgcgtg gttctttcat
    21841 cgcttttcct ggcggtcgcc tgcgccattg gccttgggtc cgccaatgcc gccgacgcgc
    21901 ctctcacagg gactttgaag accatcaagg ataacggcgt cgtggtcatc ggttttcgtg
    21961 acgcgtcgat tccgttttcc tactatgatt cgaatcagca gcctattggc tactcgatgg
    22021 atttctccaa tctcatcatc gacaagctca agcaagactt gaagatgccg aacctgaagg
    22081 ttcgcaagat tcccatcact tcccaaaacc ggatcgcatt gttgcagaac gggacgttcg
    22141 actttgaatg cacgaccacc acgaacaacg aggcccggga gaaacaagtg gatttctcgg
    22201 acagcttttt cgagattggc acgcgcctgc tgactctcaa gaattcgggc atcaaggatt
    22261 tcgccgatct gaaaggccag acagtggtcg tgggtgcagg caccacatcc gagaaaatcc
    22321 tgcgccgcat gaatgtcgag aaaaagatgg gcatgcacat catcagcgcg aaggatcaca
    22381 cggaatcgtc cttgatgctg aaaacggggc gcgccaaggc catgatgatg gacgacgcct
    22441 tgctcgcggg tgagcgcgca aaaatgaaga ccccctcaga ctatgtcatt acgggcaccc
    22501 cgcaatcgca tgaggtctat ggctgcatga cgcgtaagaa tgatgaaggc ttgaagaagc
    22561 tcatcgacgg cgtcgtgtcg aaggcggaga cgtccggcga ggcggctaag atttataaga
    22621 aatggttcat gtctcccatt cctccaaagg ggctgaacct cgatttcccc atgtcggatg
    22681 acatgaaggc tttgttcgcg catcccagcg acaagcctgt gtcgtgatga aatcggggtt
    22741 cgggctgttc gagggacgcg ccgcaagctg agggtacgat ggattacaaa tggaactggg
    22801 cggtcctgct acagcccgtg gccacagggg agcccaccac ctatttgggg tggctggcat
    22861 cgggtttcat gaacacggtg tacctgacct tgctggcgtg gcttctggca ttggccgttg
    22921 gttcggcttt tggtgtcatg cggacgttgc caggcagaat tccacgggtg ttgggcacgg
    22981 cctacgtgtc ggtgttccgg aatgttcccc tgatcgtgca gttcttcatc tggtatttcg
    23041 tcgttcccga gttgttgccc aaagcgctgg gggactggat caagaacctg gatcccaaca
    23101 cccagttttt cgtggtctct gtgttttccc tggggattta caccggctcg cgactttgcg
    23161 aacaggtgcg ggccggtatc cagtcgctgc ccaaggggca gcgcaatgcg ggcctggcca
    23221 tgggcctgac cctggcgcaa acgtaccgcc acgtgctgct acccgtcact tttcgagtca
    23281 tcattccgcc cttgacgtcg gagttcctca tcatttccaa gaactcggcc gttgcctcca
    23341 ccatcggcct gctcgagctt tcggggcagg cgcgtcaatt ggtggactac accgcccagc
    23401 cctatgagtc cttcatctgc gtgacgctgg ggtatttcct gttgaatttc ctgatacttc
    23461 gcctgatgga ctggatccgc cgacggacga gtgtcccggg cttcctgggg agctgacatg
    23521 ttccagatgg actggaagac cttgtttgaa tcccgggatc tgctgctgac aggcatgtgg
    23581 gtgaccgtgc gcatcacatt catcgccatt ctgtgcggcc tggcgtgggg gacgattctg
    23641 gctgtgtttg gcaccgtaag gaacaaggcg ctcaacctgt tttcattgtg gtacgtgtcg
    23701 ttgttcaggt ccatcccgct ggtcatgctg ctgctatggt tcttcctgat cgtgccgcag
    23761 ttcctgaagg cgttattcga tctgccaccc accgtcgaca tccgcctggt gtccgcgatg
    23821 gtcgcctatt cgctgctcga ggcggcgttc ttttgcgaaa tcatgcgcgc tggcattgtc
    23881 agcctgccca aggggcagcc ggcggctggc tacgcgctgg gcatgacgcc ttatcaatgc
    23941 atgcgttatg tggtgttgcc ccaggcgttc gctgccatgc tgcccatcgt gctcacgcaa
    24001 tgtatcgtca tctttcagga tacgtcgctg gtgtacgtca ttgctctggg cgatttcttc
    24061 agccgtgcgg aaagcatcgg ggcgcgtgac ggcacgatca ttcccatgct gatcttcgct
    24121 ggcgtggtgt actgggtgat ctgttcctcg ctcagcggcg tggtgcgcct ggtccgggcc
    24181 cgccggcccg gactttgatg gctgccgcta cggcaggctg gtttgccgca ccgcgtgttc
    24241 ccagcgctgc atcagggctt ctgcttccct tctgtcgagt cggggggtga acgtgcgttc
    24301 gacgcgccat agtctcgaca gtgcttcctt gctggtgaac gctccgcagg ccagccccgc
    24361 cagatacgcg gcgcccagtg ccgtggtttc gatgatggtc gggcgcacca ccggaatccc
    24421 cagaagatcg gcttgaaact gcatcagcaa gtcgttcacg caagccccgc cgtccacgcg
    24481 caattcggcc aatggcgcgg caccggcctg aacggcgtcg cggctcatgg cctgcagcag
    24541 cgcggcgctt tgataggcga tgctgtcgag cgcggcacgg gcgatgtgcc cgacgttggt
    24601 gccgcgtgtg atgccggtga tcgagccgcg cgcatccggc tgccagtacg gggcgcccag
    24661 gccggtgaac gccggcacca ccatgacgcc gcccgagtcc ggcacgctgc gggccagctt
    24721 ttccacgtcg ccgctgctgg ggatggcgcg caggccgtcg cgcagccact gcacgaccgc
    24781 gccgcccacg aatacgctgc cttctatggc gtattcgggc tggctggtgg tctgggccgc
    24841 gcttgtggtg agcaagccgt ttttcgatac ctggaacttg gcgccggtgt gcatcagcgt
    24901 gaaacagccg gttccatacg tgttcttggc catgccttcc tggaaacatg cctggccaaa
    24961 gagcgcgctt tgctggtcgc cggccacacc acatataggg atggcgtggc cgaacagctc
    25021 ggtgtcgacc gtgccgtatt cggtgctgga ggggcgcacg tcgggcagga gagagtgggg
    25081 gatgtccagc gtcctcagca gttcggcgtc ccactggttg tcgtgcacat tgaagagcat
    25141 ggtgcgggag gcgttgctga catcggtggc atgcacccgg cccttggtga gttgccagat
    25201 cagccatgtg tcgacggtgc cgaacgccag tttcccccgt gccgcgagtt cgcgtccgcc
    25261 agagatgttg tccaggatcc actttagttt ggtggctgag aagtaggcat ccacgcgcag
    25321 tccggtcttt tgcaggatgg tgtcttccaa cccccgctgg cgcaagtccg cgcaggcggg
    25381 ctcggcgcgg tgatcctgcc agacgatggc gttgcaaagc ggctgcccgg tttccctgtg
    25441 ccagacgatg gtggtttcgc gttggttggt gatgcccgcg gccagtatgt cacgggcttg
    25501 caggccggcc ttggccagcg cgtcgcgcgc ggtccgcaac tgggtggacc agatatccat
    25561 cgggtcgtgc tccacccagc cgggtcgtgg gtagtgctgc gtgagctcct gttgggcgag
    25621 agcaacgatc cggccggtgt cggtatcgaa aacgatgctg cgcgaactcg aagtgccctg
    25681 gtcgaaggca agcagatagg ccatggtcgt tatgggttcc tgtgggaatc ggtcatgggg
    25741 atcccgccac atcgcagcgc acgtgcactt gggcaagaag gtcggggaaa gggtctggcg
    25801 gcgtggcgtc ggtgaacagc cgatcgacct gggacagatc ggccaccttg accatcgcct
    25861 ggcggttgaa cttcgtggcg tcggcagcga gccagatttc ccgggcgtgc tcgatgatgg
    25921 cttgcgaaac cttgacctcc ctgtagtcaa agtcgcgtag cgatccgtcc ggctcgatgc
    25981 cggaaatgcc gatgagcgcg atgtccacct tgaattgccg gatgaaatct atggtggctt
    26041 cgccgatgat tccgccatcg cgggagcgca ccacgccgcc cgcaacgatg acttcgcagt
    26101 tgcggttgcg gctgagggtg gccgcgacat tgagatcgtt ggtgatgacg cgcagccccg
    26161 tgtggtgcat gagcgcccga gcgatggcct cggtggtggt gccgatgttg atgaaaagcg
    26221 agcactcgtc cggaacggcc tgcgccacgg cgctggcgat gcgacgcttg ccggacgtgt
    26281 tcaggttttc acgctggtga tgcgcgatgt tctcggttgt cgagctggga acgctgacac
    26341 ccccatggaa gcgtgcgagc aagcctgcct cggccagccg ttgcacgtcg cggcggatgg
    26401 tttgagctgt cacgccgagc cgtatcgcca gttcttcgac tgtggaggaa tgctgttcct
    26461 ggacgtgcct gagtaaccgc tgttgtctgg ggttcggtgt catgcggtca tccgtggcgg
    26521 caatgcttgg actatcatgg ctcaaactgt acaccgaaac gaaaagaaat agtgattcca
    26581 acggctggtc gtcttgggtg tctttggtgg tgttcttttc taaaacgaac gataataatc
    26641 aaaaatgaac gaaaccgggc agactcattc ccacccactc tgtggacgcg tttgccattt
    26701 cctccgcact gtttgccatg atgcccgcct ccgaacctct ccttactgat cgatcccggc
    26761 tcttgtcggc attgtccgaa cctatgcgat acgacctggc cgtcattggc ggcggggcca
    26821 gcgggctggg tgtggcgctg gatgcggtgt tgcgagggct gtcggtggtc ttgctcgaat
    26881 cacacgattt cgccaagggc acttcctctc gcgcgaccaa gctggtgcat ggtggtgtcc
    26941 gctacctggc gcagggcaac gtgtccctgg tcagggaagc gctgcgcgag cgcaccgttc
    27001 tgttgcataa cgcgccgcac ctggcccggc cgttgccgtt cgtcatgcct tgttatagct
    27061 actgggaaat gccgttctac ggttcggggc tcaagctata cgacctgctt gcagggaaag
    27121 cgggcctggg caatacggaa atgctgggcg tggctggcac cgaacgttgc ctgcccggcg
    27181 tgcgcacccg ggggttgaag ggcggggtca aatactggga cggtcagttc gacgacgccc
    27241 gtctggcact gacgctggcc cgcaccgcgg cggcgcgtgg ggctttgctg gtcaattact
    27301 gcgccgtgac cggcttggtg catgaacaag ggcgtatcgc cggcctgcgc gccaaagacg
    27361 ctgaaaccgg cgccgaattt caaatatcgg cccgctgcgt ggtgaacgcc gccggcgtat
    27421 gggtggatga aatccgccaa caagacgggc aggccatgca tcgtgccgcc aaacccatgg
    27481 ttgcgcccag ccagggcgtg cacctggtgg tcgatcgcga ttttctgcgt ggcaatcacg
    27541 cgctgctggt ccccaagacg cgcgacggcc gcgtgctgtt cgccgtgccg tggctgggca
    27601 aagtcgtgct gggcaccacc gacacccccc gtcacgacct gtcgcgtgaa ccggatgcgt
    27661 tcgccaacga agtcgatttc atccttggcg aggctggtaa ctacctcagc cgacgcccca
    27721 cgcgcgacga cgtccgcagc gtctgggtcg gcctgcggcc cctggtgcgc ccgcccgatg
    27781 acgagggtga caacaccaag aaaatcagcc gggaacacac ggtgctggtc agcccaagcg
    27841 gcctggtgac ggtcaccggc ggcaaatgga ccacttatcg cgccatggcc gaggatgtcc
    27901 tggccaagtg tgtggaggcg gggttgtttt cctcgctgcc acccagtgtc accgccacgt
    27961 gtgttctggc gggggcggtg gcgggtgcca cgtatcgcgc caccctcacg gaaacgccag
    28021 gcgccgaggc ctatggcgag gacgcgcaat ggctgcgcgg cctggatggc catgatgtga
    28081 cgcttgccga cggactcacc gagtccatgg tgcgcttcgc ggcgcgttat gaatacgcgc
    28141 gctcggtcga agatatgctg gcgcgtcgtt accgcctgct gtttcttgat gcgcgcctgg
    28201 cggcgcatgt ggcgccgcgc gtggccgagt tgctgcgcga ggaactgggc gtggatcccc
    28261 gtctggacga tttcctgcgt cttgccgacc actatctgcg cctgcccgct ggttcgtgac
    28321 acgttattgg ccaattcgcc ggtcccgcct tcgggcgcgg ttcactgcaa agtgtcactt
    28381 ggcgccgaca gatgtcaatc ggcggcgaac aaggtgcgcg caccgtcgaa gcgccgggca
    28441 tagtagcggc tgttgagcga gtcgatgcgg accacgccgc cgctcgatgg ggcgttgatg
    28501 aaacggccgt ggcccagata gatccccatg tgcgaatagc ggcggttcag ggtgttgaag
    28561 aacaccagat cgcccggact cagggccctt ctgctgatgg ggcggctgat ttcggcgatg
    28621 cgtgccgtat tgtgcggcag accggcgtcc gccgccgcct tgaatacata atccaccagg
    28681 ccactgcagt cgaagccggt gtccggtgaa tcgcccccgt atcgatagcg tgtatccagt
    28741 tgagcaaggg ccatcatgac cacatcggtg cgcttgcttt gatcaaccac caccggctgg
    28801 ccacgccaat accccgcgcc ggactcgtgt ctgggagtgc tggcgcagcc cgcgagcagc
    28861 gtcagcacgg ccaccaagcc aggaaacagg aatcgtcgga catactgtgg catgggcgcg
    28921 atcattttcg cattattact gtaatttcat gtggatacaa gactttattc acgctgttta
    28981 tcgcatgatg gcctatggcg tgcaggaatc gcgagagggg ataccgtgca gaaccaggcg
    29041 tcgaccgacg attgggaaaa tatcggtgag gagtgggtgt cagacttgat tttgtgacga
    29101 tgctgtttca aaaaatgtgg tatttcagtc gcaatcttca attaatatgt aacatagata
    29161 cgtgtacgta attatgtaac ataactaacg gttgctgaat atcttttaat ttcttttaaa
    29221 aaacaaaaac aatccattaa tttctcaata aaaataacaa tattaagata tctgtcaata
    29281 ttgatattta tgcgaaatac acagattaat ggcaatatga tacaaaaaac aggttttgtt
    29341 agttttggac catgatgcgc ccgcctatgc ggaatgtggc ccgcatcata ctcgcgaacc
    29401 ctcgattttt tccctggaac accttctttg tcacaggagc ccaacatgct ggctgtcgat
    29461 gattcattgc tgagcgacat tcaagaagtc aatttgtcgt accttttgct tgcccagcgc
    29521 ctattgcgcg aaaactacgc gtcggccatg ttccgcctcg ggttcactga agacgtggcc
    29581 gaagtggtgc ttcaactgtc ccccgcccag ttggtcaagc tggccgggtc gggttcgctg
    29641 atctgtggct ttcgtctgaa cgattacgat ttgttgtcgt cgctggcgaa tgacgtgctg
    29701 gggggggtgt tgcagcaggc gcatgccacc attcttctgg cgcagcgctc cgcgcttccc
    29761 caggtggctt gaatcacgtt tttgcggaag tattttcatg tccacgaaaa gcgtttcccg
    29821 cgatgccgag gaaatcctgc tggccaccga tatgatcaaa ctcggggcca ggcttcaggt
    29881 gctgcaggcc gaaaccagcc tcagctacga tcggctcgcc cgcctgtatc gggagatcaa
    29941 gggttgctcc ccacccaagg gtatgctgcc attttcggtg gactggttca tgacatggtt
    30001 gccgaacatc cattccagtg tgttctacaa catttaccgg ttcatggacg cccatacacc
    30061 cgccaagaag tccggggcgc tcatagaagg ctaccgcctg tatctcgagc aggccgacgc
    30121 tggacggctt gcgtcgtcgg ctaaagagcc tgtgctgagt ttcacccgtg cctggatgtt
    30181 ggtgcgtttc ttcgacagtg atctggtgca gttatcccgc tgtacccgtt gcacaggcca
    30241 gttcgttgct catgcccacg atccccaggc caattttgtt tgcgccatct gccggccgcc
    30301 tccgcgtgcc ggcaaaaccc gcggtcgggc tgttttgcgc cagccggcca aagtgtagtc
    30361 agggtgaagc ggtacaccgt cgttgtccga gcggcagccg agccccactc ctcctcgctc
    30421 cggtatcttt gtaggcgccg atctggcctc gaaaatccct gttgtcaccg gttttcgcca
    30481 agaccgaagc agaggatcat tgcaaccgtt tggtcgataa aggctcgctt gtgttaatcc
    30541 tgcttggctt cctggttgtc ttggtctctg ttttcggcag ctacacgttt ctgggtggcc
    30601 atctcggggc gctgtaccag ccgttcgagt tcgtgctcat cgccggggcg gcgctcggcg
    30661 cctacctggc gtcgaacagc ggcaagtcga ttgggttgct gttgcgcgcc gttcccaacg
    30721 cgtttcggcg cagcccctat gacaagccac tctacatgga gctgatggcg ctgctgtatg
    30781 tgctgctgaa caaggcccga cgcgacggcc tgatgtcggt tgagtccgac attgaagatc
    30841 catccgccag tccggtcttc gccgagtatc cgttggtact gcgcaatgcg cgtctgatgg
    30901 aattcatcac cgactatctg cgcctcatca tcagtggaaa catgagcgcc ttcgaaatcg
    30961 aaacgctcat ggaccaagag atcgaaacgt tgcgcaagga actacacatt ccagtgcatg
    31021 gcctgcgcgc cgtggccgat gcgttgccgg cgttcgggat cgtggcggct gtgctgggtg
    31081 tgatcaaggc cttggcggcg gtcgatcaac ccccggcgat tctcgccgat ctgatttcca
    31141 aggccatggt gggtacgttc ctaggcatcc ttctggctta cggcttcgtg gcacccatgg
    31201 cctcatccat cgagcgccgc aatgctggcg tcgagaaagt gcttgaatgc atcaagctca
    31261 cattgctggc caacatgaat ggctatccgc cgcagctggc tgtggagttt ggccgcaagg
    31321 ccttgtattc cactgttcgc ccttcgttcg tcgagctcga ggaacacgtt cgtcagaccc
    31381 ggtcgctggc tgggaagaaa gcatagcggg ctgcggatat gagttcacgc ggaccggagc
    31441 gcatcgtcat tcgccgcggg cacgcgcatc ttgacgaaag ccacggcggc agctggaaga
    31501 tcgcttacgc cgacttcgtg acggccatga tgtcgttctt cctggtgatg tggctgatct
    31561 ctgtcgtgcc gcacaaggat ctccacgcca tcgccgaata cttccgcatg cctttgatga
    31621 ccgccgtcac gggtgggccc aagatcgact cttcaagctc ggtgattccg gcgggctcac
    31681 ccagtgtcat accgaacaag aatccgctgc ctcgtcagca acacgatgac ggaacgaccg
    31741 aaagcgacaa gcgcgacacg cagcggctgg agggcctaaa aagcgagctc gatcaactga
    31801 taaagtctga tccaatcctc aagcagttcc gcccccagct attgctcgac atgacaccgg
    31861 acgggctgcg catccagatc gtggaccagc agaaccgtcc catgttcgat accggcagcg
    31921 cgaagatgca gccctacatg agtgccatac tccgggaatt ggcgccggtc ttcaacaaac
    31981 tgcccaacaa gatcagcata gccggccaca ccgatgcggc ccagtattcg acgggtgagc
    32041 gggaatacag taattgggag ctctccgcgg accgcgccaa cgccgcccgg cggcaactga
    32101 tcgccggtgg catggatgag ggcaaggtca aacgcgtgct ggggctgggt tcgacggtca
    32161 gcctgattaa agatgatccc agcgcggccg ttaataggcg catcagtctg gtggtgttga
    32221 acaagcgcgc ggagcgccgc atcgatgcgc agaacaccac tgcggccgcc gtgctcaagc
    32281 tccccgagct cgaacacctc gctcagcccg cgctggcggc gccgtcagac tgaggctttc
    32341 agcaaccgac aggggcaagc atgagcgctg gagtggatct cagccagttt ttcgacacat
    32401 tcttcgacga ggctgacgag ctgctcgccg acatggagcg tctccttctt gggttggacg
    32461 ttgatcagcc cgattcggac gatctgaatg gcatattccg ggccgctcac tccataaagg
    32521 gcggtgccgg cacgttcggt tgctttgacg cgttgacaca gaccacgcat ttgctggaaa
    32581 acattctcga tcaggtgcgc cggggagaca tggcgctgcg caaggaaatg atcgatcttt
    32641 ttttggaaac caaagacgtg ctcgcggaac aggttgccgc ctacaggagc tccgggaatc
    32701 ctgacgcaca ggcgtatgag cgtatctgcg cgcagttgcg cgagttggcg ctggcgaatc
    32761 aaacagatgg tgtggacgat ggcgttccag ccatgcagca ggccgggccc ctggcccccg
    32821 cgccggcctc gaccagtgtc gtggcggttg ctgtttcggc cgttgcaggt ggaaagtccg
    32881 tgaaacgggt cgatctgacg ggggtcgcgt cggccgatgc gcaatcgctc atcaccgaga
    32941 tggcgctgct tggcgaggtg gtgcgccacg aagaccgtgg cggtgttgtc tccatctggc
    33001 tcgaaacccc ggaaagcgct gacgacatca ccgcggtctg ctgcttcgtc gtcaacgccg
    33061 atcaggtacg ggtgacgagc gaggggggtg ggttcgcggc ggagcccgtc gtcggtgcgc
    33121 agcacccacc ggcaccgcct gtggcgggtg cacccgcacc cggatcgcct ctggcgccgg
    33181 aacccacgtc cgcgggccac gaaggccgtg aggaaaggcg ttccggtgtc gatcgccgaa
    33241 agaccacgga ttcggccgcc cgcaaagagt cgggcaccat ccgggtgggt gtggagaaag
    33301 tcgatcagat catcaatctg gtgggtgaat tggtgatcac ccaggcgatg ctggtgcagg
    33361 caggctcgga actcgatcct gtcgtccacg atcgattgct caatggcatc gagcagctcg
    33421 agcgcaacgc acgggatctc caggaagcgg tcatgtccgt gcgcatgatg cccatggatt
    33481 acgtgttcag ccggtttcca agagtcgtgc gggaaaacgc gtcgaggttg ggtaagcgaa
    33541 tccggctggt gacccagggc gaggcgaccg aactcgacaa gagtctcata gagcgcatca
    33601 tcgatccatt gactcatctc gtgcgcaaca gcctggatca cggtatcgaa gcgcccgacg
    33661 tgcgcctggc gcgaggtaag aacccggaag gctgcatcac actgtcagcc cagcacaacg
    33721 gggggcagat catcatcgag gtgtccgacg acggcgccgg gctcgatcgc cgacgcatcc
    33781 tggccaaggc ggcgtcgcag gggatggcgg tcagcgacac cgcgagcgac gaagaagtgt
    33841 ggcaactgat tttcgcgccg ggtttctcga ccgccgaaaa aatcaccgat atttccgggc
    33901 gtggcgttgg catggatgtc gtgcgccgca atattcagga tatgggtggc catatccagc
    33961 tgtcgtcgcg ggaagggcgg ggcaccatca tgcgcatcgc tctgccgctg accctggcca
    34021 ttctcgatgg catgtccgtg aaggttggca atgaaacatt cattcttccg ctcagccacg
    34081 tcacggaatc catgcagccc accaccgagc agattcacca catctcggag ggcgagcaac
    34141 tcatgtatgt gcggggtgaa tacctgccca tcgtcgcgct gcacgaggtg ttctccgtcg
    34201 agggcgccca gaccaacccc actggtgcga tcgcggtgat tctgcaggcc gaggacgcgc
    34261 gcttcgcctt gctggtcgat caactcgtcg gccagcatca ggtggtcgtg aagaatctcg
    34321 aatccaatta ccgaaaaatc cccggggttt ccgccgcgac cattctagga gacggcagcg
    34381 tggccctgat cgtcgatgtg ttcgccctga tgcgcatgac gcgcgaaaca gcgtgaacac
    34441 gcgcccagaa ctggagaaaa tcatgttgaa cagattcgag tccgatgccg ggcaggtcga
    34501 caccgttgga aaggaattcc tggtgttcac gctggcgtcc caggaatacg gaatagacat
    34561 actcaaggta caggagatcc gtggctacga cagccagacc gtgacgcgca tcgccaacgt
    34621 gccgtcgttc gtcaaagggg tcaccaacct gcgcggcgtc atcgtcccga tcgtggacat
    34681 gcgcatcaag ttcgatctcg aacgtgtcga gtacgaccac cagacggtcg tcgtcatcct
    34741 gaatctgggt tcccgtgtcg tgggcgtggt ggtcgacggt gtgtccgacg tactgatgct
    34801 gcagtcgtcc cagatcagca cgccgccgca gttcggtaca gcgttctcca cagaatatct
    34861 cactggcatc ggcacactgg gcgagcgcat gctgatcctg gtggacatag aacggctgat
    34921 gaccagcacc gaaatggcgc tggtcgagca ggcggttgcc tgacagccat gattcgtgcc
    34981 cgccgtggta acgcaccacg cgtccattct ttcgcatcat ttccgtttcc gacatgagaa
    35041 aacccgttct attttccaat ttcaaggtgc gcaccagtct gattctgatg ctgctgtttt
    35101 tcctgtgcat gctgttgtcg ggggccgcgc ttgggttgtg gtctttgtat tccaacaacc
    35161 aggcactcca gcgcattgct gacaacgagg ccatagacag cacgcttagc cagtccatcg
    35221 accagtacaa ggatatccag ggcgcattgg ggcgcgcgct cgcgagttat gtggtcaatc
    35281 atgacaagcg acattctggc gcggccgggc aggcggaggt ggccgcaacc acctacacgg
    35341 gtgcgctgga ccccgaatcg caaagccagg tgaagaatgc gcgccaggcc atgagtacct
    35401 cccagagtat cttcgatact tatcgcaggc tgaccaaagg acgcaaggat ggcgtctatc
    35461 gcgatctgga acaatcatat gacgcgttga tggctggcgg tgtcacgccc ctgttcggat
    35521 tcctcgccaa gggtgacatc agcggctatc acgacttcct gtccaccacc agcagcagtc
    35581 tggaagacga tttctaccag accctgggcg tcgtgcacgc gcatcaacgg cagctcatcg
    35641 ccgactccga ggcggcggaa cagatgcatt atcagcgcgt ggtgatcctc gtcgccattg
    35701 ccgtcacgct gtcggtgctg atcgcgttgc tggcctatgc ggtgctggga cgtgtggtgt
    35761 tgcggcctct gcgtgccgcg cagtcgcact ttgagcgcat cgccgcgggc gatctgactc
    35821 aacgcataga ggtgcggtcg cgcaatgaaa tcggggtgtt gttcgatgcg ctgcgccgca
    35881 tgcaggaaag cctgaccggc accgtgtcgt cggtgcgaca aggggtggag gaaatcacgc
    35941 tgggctcgca ggagattttc gcgggcaaca ccgacctgag ctcccgcacc gagcagcagg
    36001 cggcgtcctt gcagcagacc gcggccagca tggaagagct agccagcacc gtcaagcaga
    36061 acacggacaa cgccatgcag gccgaccaac tggccaagaa ggcctccgaa gtcgcgcagc
    36121 gtggcggcgc cgcagtcggc gacgtggtcg gctcgatggc cgccatttcc gcgagttccg
    36181 gaaagatctc cgagatcgtt ggtgtcatcg atggtatcgc gttccagacc aatatcctgg
    36241 cgctgaatgc cgccgtcgag gccgcgcggg cgggcgagca gggcaagggc ttcgccgtgg
    36301 tggccgggga agtccgttcg ctggcgcagc gcagcgccca ggcggccaag gaggtcaaga
    36361 cgctgataga ggaatcccgg tcccgcgtgc aggcggggtc cgcgcaggcc atacaagccg
    36421 gagacatcat gcgggaagtg gtgggggccg tcgagggtgt gaccaccatc atgagcgaga
    36481 tctcgtcggc gtcgcgcgaa cagtccgacg gcatcggcca ggtcaatcag gctgtcgcgc
    36541 agatggatga ggtcgtgcag cagaacgcgg cgctggttga gcaggccgcg gcagcttccg
    36601 gctctctgca ggatcaggct gcccgcttga atgacgccat ggcagtgttc aagatcaaca
    36661 caagcgaaat catcgatgtt cccgcagccg cgctggccca acctgacaaa cccggcgggc
    36721 ggatggcagc ggcctatgcg cctgccacct tggctggcca gatatcatga gcgccatgcc
    36781 ccgtgccgaa tcgttctcct tgctggcggg tctgcccgag gccggcgacg acgatatcac
    36841 gctgtcaacc cggctgctca aggcgcgcgc ggggttggtt ctcggggcgc acaagcgtga
    36901 catgataggc cgggtgctgg gcctgcgcgc ccgtgacctc ggcgttggga ccgtcaccga
    36961 ttacctgacc tcgttgcagc ggggcggtga aagcaccgaa tgggaacact tcgtcaatgc
    37021 cttcaccatc aaccacacgg ccttcttccg tgagcagcat catttcgatt cgttgacgaa
    37081 attcgcccgc gcgtgcaaga cgccgatctc ggtgtggtcg tgcgccgcct ccaccgggga
    37141 agaagcctat tccatcgcca tgacgctgcg cgaggcgcag cccgctaccg acctgcgcca
    37201 ggccgtctgg gccactgata tcgatacgca ggccatcgcc cgtgcgcgag cgggggtgta
    37261 ttccggcgat cgggccgagc ccattcccga ggtgttgctc aagcgctatt tccagcgggg
    37321 ccagggggcg cgcaccggac aggtgcgggt caaaccgctg ctgtcgagcc tggtgcggtt
    37381 cgatacgttc aatctgtgcg ctcccgcgtg gccatcgggg cgcatgttcg acgccatatt
    37441 ctgccgcaat gtcatgatct atttcgacaa ggaaacgcag gtacgcattc tcgaccatat
    37501 ggcaaccatg ctgaggcctg gcgggctgct gttcgccggg cattccgaga acttcaccta
    37561 tctgaccgat aaattccgcc tgcttggcca gacagtgtac gagcgggaca agctcaaatg
    37621 atcagtgctg atcgccaagc tccagggcag acggctgtct ccggcaagat ccgggtcctg
    37681 tgcgtggacg attccgcgct ggtgcgcggg ctcatgacgg aaatcctcaa cggccagccc
    37741 gatatcgaag tggtggcggt ggcacccgat cctctggtgg cgcgcgaact gatcaagcgc
    37801 cacaaccccg atgtcctcac cctggatgtc gagatgccac gcatggacgg tctggatttc
    37861 ctcgaacgcc tgatgcgctt gcgaccgatg ccggtggtca tggtgtcgtc gctcacggaa
    37921 cgcaattccg atgtgacgct gcgggcgctg gaactgggtg ctgtcgattt cgtcaccaaa
    37981 ccgaaacttg gattgcggga tgggttgctg gaatactccg atctgatcgc cgacaagatc
    38041 cgcgcggcgg cccaatcgcg gccgcggcaa cgtcagccgg ttcaagcggt cgcggtccgg
    38101 cgcctcgccc gacccttttc caccaccgaa aagctgattc tcatcggcgc ctccacgggt
    38161 ggcaccgagg ccatacgcca ggtgctcgag ccgttgcccg ccaacagtcc cgcgatattg
    38221 atcacacaac acatgccggc cggcttcaca cggtcattcg tgcgacgcct ggacagcctg
    38281 tgcgccgtgc aggttcatga ggccgaggct ggtcagcgtg tgctgccggg ccatgtctat
    38341 ctcgcccctg gcggggtggc gcacatgagg ctctcgcggt cgggcgccaa ttacgtcgtc
    38401 gatcttgacg acaccgagcc ggtcaatcgg catcgtcctt cggtcgatgt gctcttccat
    38461 tccgcggcgc aggtggcggg gggcaatgcc gtgggagcca ttctgactgg catggggcgt
    38521 gacggcgccc aggggttgct cgcgatgcga aaggccggtg ccatgacttt cgcccaggat
    38581 gaagccagtt gtgtcgtgtt cggcatgccg cgcgaggcac tgttgatcgg tgccgccgcc
    38641 gaggcggtgc cgctggcgga gatgggcgag cgcctgttgg ccagcgccgg cgcctatggg
    38701 catcgcgtgt gaaccttggt tggccttgtt tctatagatg gagatgattt gatggagatg
    38761 atcacgtggc acaaaagtcc ataaaaattc tggtggtcga cgattttccc actatgcgca
    38821 ggatcgtcaa gaacctgctc aaggatctcg gatacgagaa tgtcgacgag gccgaggatg
    38881 gcgccatggc gctcgagaag ctgcacaccg gcaatttcga cttcgtggtg tccgactgga
    38941 acatgcccaa catggatggg ttgagcatgc tgaaggcgat tcgcgccgat gagcagttgg
    39001 cgaggcttcc cgtcctgatg gtgacggccg aggccaagaa agagaacatc atcgccgccg
    39061 cccaggccgg cgcgagtggc tatgtggtca agcccttcac cgccgcgacc ctcgaggaga
    39121 agctcaacaa gattttcgaa aaacaccaga tatagcgctc tggaacgtaa aaggaaacca
    39181 tcatgcatac ggcagagtcg tgccaaccgg aggttgacgc cgggcagccc gtcgatttga
    39241 tctatcgtat cgcccagctc acgcgcatgc tgcgcgaaag catgcgcgaa ctcgggcttg
    39301 accaagcgat caaggatgcc gcgcaggcga tcccggatgc ccgggatcgc ctgcgttacg
    39361 tcgccagcat gaccgaacag gccgccaacc gtgtgctgaa cgcggccgag aagattcaac
    39421 ccatccagga tgggttgcgt cgtgatgcgc aagcactcga cacgcgctgg caggcctggt
    39481 tcgaccaccc cgttgaactg gaacaggcac gcaccctggt ggatgacacc cggggactgc
    39541 tgcggagcat ccccgatcgg gcccaggcgt cgcaggactc cctgatggaa atcatcttgg
    39601 cacaggattt ccaggatctg acgggccagg tcatcatgaa gatgctcaac gtcgtcgggg
    39661 ccatagaaac cgaacttgtc caggtgctgc tggacaatgt gcctcaggac aggcgcaacg
    39721 acacggcctc tctgctcaac ggcccccagg tctctcccgg cggccggccg gacgttgtca
    39781 ccaatcagga tcaggtcgac gacctgcttt ccagcctggg attctgacgc gcggcgcgac
    39841 tggaagtgaa ttgagcgggt ttcccaggct tgctccgcgt atcgttcctg gcccgttcct
    39901 ctagaatttt cggattgacg aaactctgga tgcgacggcc ggatggctga ggaaagcgat
    39961 ctcgaaaaaa ccgaacccgc cactccccgg cgcctggaaa aggcgcgcga ggaggggcag
    40021 gtcgtccgtt cgcgtgaact caacacgttc ctgttgctgc tggcgggggt cggtggcctg
    40081 tgggcgggcg cgggtgacat ctatcgcagt ctgtacaccg cagtgcatgc gacgctgtgg
    40141 atcgatcccc gcatcgcgcg cgacaccggt gtcatggcga cccgggccat cgatgccggt
    40201 cttggcgtgg catgggcgtt gctgccgata ttcgcgctac tggtagtcgt ggccctggtg
    40261 gcgcccatgc tcctcggggg gttcctgtta tccagcaagg cactcgctcc taaatttgaa
    40321 cgcctgaacc cggtaaaggg attcgtccga ctgttctcgt cgcagacgct gatcgagctg
    40381 atcaagacgc tcgtcaaggc ggtgctggtg ggaacggtcg gcgccatggt catctggcat
    40441 taccgtgacc gcatgatcgg gctgatgtac gccggctcca ccgaggcgct ggccgagggg
    40501 ctgaatctgg tcgcgttgtg cagcatcctg atagtcggtt ccctggcgtt gatcgtgctg
    40561 atcgatgcac cctggcaact ctggagccat ttccgcaagc tgcgcatgtc gcgcgaggac
    40621 gtccgtcagg agaacaagga gagcgaaggg gaccctcacg tcaaggggcg catccggcag
    40681 cagcagcggg cgatggcgcg ccgccgcatg atggcgcagg tgcccaatgc cgacgtggtc
    40741 gtgaccaacc ccacgcatta cgcggtggcg ctggcctata gcgaagggcg gggcgccgcg
    40801 cctcgcgtgg tggccaaggg ttcgggcccg atcgccgccc gcatccggga aatcgccgca
    40861 ggcaaccatg tgcccgttct gtcggccccg cctcttgccc gggccctgtt ccatcatgtc
    40921 gatctgggtc gggagattcc caccgagctg tacgcggcgg tggcgcaggt gcttgcctgg
    40981 gtgtatcagt tgcgacgctg gaacgccgga caaggcgtgc agcccgtcgt ccccgatggg
    41041 ctgattgtgc ccgagggcat ggatcccgga tccgcgccag atgccacggc ggtcgtggca
    41101 tgaacggcct gatcgccctg ctcaagacca atggcgcgca gaacgcgcgc ctgatggccg
    41161 gccccatcct gatcctgatg gtgctggcga tgatgatcct gccgctgcca ccgttcatcc
    41221 tggatttgct gttcacgttc aacatttcgc tcgcggtgat gatattgctg gtggcgatgt
    41281 tcaccaagaa gccgctggat ttcgccgctt ttccctccgt gctgttgttc gcgacactgc
    41341 tgcgcctggc cctgaacgtg gcctccaccc gggtcgtgct gctcaacggc cacaaggggc
    41401 ctgccgccgc cggccacgtg atcgaggcgt tcgggcattt cctggtgggt ggcaatttcg
    41461 cggtgggcat cgtggtgttc gtgatcctgg tcatcatcaa tttcgtggtg atcaccaagg
    41521 gcgccggccg tattgcggag gtgggcgcgc gcttcacgct ggatgccatg ccgggtaaac
    41581 aaatggcgat cgatgccgat ctgaacgccg gcctcatcgg tgaggacgag gcgcgccggc
    41641 gacgcgccga agtcgggcag gaggccgaat tctacgggtc catggatggc gccagcaagt
    41701 ttgtgcgggg cgatgcggtg gccgggctgt tgatcatggt catcaacatc ctgggcggcc
    41761 tcatcgtggg cgtggcgcag catgggctga gtttttccga cgccggacgg gtctacaccc
    41821 tgctgaccat cggcgacggc ctggtggcgc agattccggc gttggtgata tccaccgccg
    41881 ccggtgtcgt ggtctcccgc gtcgcgaccg accaggacgt cggccagcag atggtggggc
    41941 agttgttcac gaaccccacg gtgctgttca tcacgtcggg gatactgggc gccatggggc
    42001 tgattcccaa tatgccgcac gtggcgttcc tgtcactggc cgcggcgctg ggcggtggcg
    42061 gctgggtcat gcgccgccaa gctcttctgg agcatgccag acaggcccag gagcccatgc
    42121 gcaagcaggc ggccgccgac gcagcggcgg cggaggccag ttgggacgat gtgtcgttgg
    42181 tcgatccgct ggggctggag gtcggatacc gcctgatccc cttggttgat cacgcacaga
    42241 atggcgagct tctgcatcgc atccgcagtc tgcgcaagaa gttcgcacag gatgtcggtt
    42301 tcctcgcccc cgtcgtgcac attcgtgaca acctcgatct caaacccaac gattaccgca
    42361 ttctgctttc gggtgtcgaa gtcggtcgcg gcacggccat gcccgggcag tggctggcca
    42421 tcgatccggg cggggccagc atgaccctac ccgcgacgcc caccaaagat cccgcttttg
    42481 gcctgcccgc gttctggatc gatgtcgcgc tgcgtgagca ggcgcagatc gcgggctata
    42541 ccgtggtcga cgcgggcacc gtggtcgcca cgcacttgaa ccatctgatg caccgccacg
    42601 gggccgatct gctcggccgc caggaggcac agcaatggct ggatcacgtc ggccgcgagg
    42661 ccccgaaact ggtcgaggat ctggttccaa agaccgtgtc gctgacggcg ttgcagaaac
    42721 tgttgcgagg gttgctcgat gaagaagtgg ccattcggga tgcgcgcacc ctcatcgagg
    42781 cgctggccga gcatgcgccc cggctcgccg ccctcaaccc gaccggtgcg gggccggatg
    42841 cgggcgaatt gctggcgcag gttcgtgtgg cgctcggccg cgccatcacg cagcaatggt
    42901 tcgcgggtga gggcgacctg tgtgtgatcg gtctcgatgc ccggctcgag aacgtgctgg
    42961 tgcaggccat cggcaccagc ggcgcactgg agcccggcct ggcggatacc ctgctgcagg
    43021 gcgccgcgca ggccgtccag acgcaggagc gtgacgggca ccccacggtt ctggtcgtgg
    43081 ttcccgctct gcgcgtggcg ttgtcgcggt ttctgcgcca ccatctgcca cagttgggag
    43141 tcttgtccaa cgtcgagatt ccggatgaac gcattctgcg cgtgaccgcc atggtgggcc
    43201 ggagcgccgc gacgtgaatc tgagccggtt cgtcggtgcc accagccggg aggcgctgag
    43261 gcaggtgcgc ctggcgttgg gtcccgacgc gctcatcgtc tccaaccggc gcgtcaatgg
    43321 cggggtcgag atcctggcgt ccgaccccac atcggcgacg tccgaagagc ccgggattga
    43381 acccaggccg cgccccgagg cgccgacgca aggtgtcatg ggagcgatcg agtcgatgcg
    43441 tggcgcgctc gaggctcgtc tcgatgaggc catgtggggt aacaggctgc gtcaggcgcc
    43501 gcaagccgcg cgcttgtttc aaagcctgct tgggctgggt ttcagcacgg cgctgctgcg
    43561 agccatgctg acgcggttgc ctgcaggtct tggccagggt gccgccatgc gttgggtgcg
    43621 tgatgaactg gcccgtcatc tacccgttct tgcccgtgaa ggcgatctat ggaaacccgg
    43681 gctggtgctg gcattggtcg ggccgacggg tgtcggcaag accacgacgg tcgccaaact
    43741 ggccgcgcgt tgcgtgcggc ggaccggccc ggccgggttg gtcctggtca ccaccgacac
    43801 ctatcgtatc ggggcgcatg aacaattgaa gcaatacggt gacatgctgc atgtgccggt
    43861 tcatgtcgtg cgcaacgtgg acgagttgcg tcgcctgctg ggggccacgc acccgtcgca
    43921 gacggtgctg atagacaacg tgggcgtgag ccagcgtgac cgctatgtcg ccgaacaggc
    43981 ggcgctgctg gcgggcggag gcaggcgggt ggatcggttg gtcgtcctga acgcctccag
    44041 ccatggagac acgctggacg aggtggcgcg gacctacacg tccgatggcg ggggggcgct
    44101 ggcgggatgc ctcgtcacca agatcgatga agcgccgcgc atcgctgccg ccctggacac
    44161 cgccatacgt tatcggctgg ccgtccatta tgtttcggat ggccagcgcg ttccagagaa
    44221 cctgcggcat cggtcgcccg cggaactggt ggacctggcc ttgacacgtg ccggaaccgg
    44281 gcgcgatctg tttgcgccaa ccgaagcgga cctcgccgcg ctcatggaga tcgccagcga
    44341 tgacgggcct cgtatcgtgg gctctaggga acaacgccgc atgcgcctgc tgcctggatt
    44401 gctgaccggt ggcgatggcc ggcagcctac cctcgacgca ttgcggcggg caagtgatcg
    44461 cctggatgat gcgcccgcct gcgcggaggc gttcgggttg tggcgcgcct tcattgacga
    44521 gggtgccacc gtcgatgcgg cggcttcgtc ccgtcatcta tcgcgctgcg cggcaggcgc
    44581 ctcggtggcg cccggaacgg cgctggcggc attgcatggg cgcacgcgtc tcgacctgcc
    44641 cgatggcgtc ccgggcgagc ttcatgcgac ggctttgttc gatgggaaca tgaggagcct
    44701 gactgttccc gcactgcagt tggcgctggc gggaagctgg caacctgtct ggggcgatcc
    44761 cgccttgact ccaccagtcg ccgaggcggc gttgctggag cggctggcat ggttggactg
    44821 taacctgcaa agcaatccac tggttcatct gctgcatgga atgagtgtca cccgggcgcg
    44881 cacgcttgcc gagggcgggt atgcctggct ggccgcctgt gcggccaaaa ccatggtgat
    44941 gtcggattcg tgcgcggcgc ccatcgcggc gcttgcgcgc gaactagttt ttcgtccatt
    45001 gttggatcca gtccgcggcg cctctgtttc ccctgatcca ctgtcgagca agtcagaatc
    45061 aactctctgg gtggctcatg cctccgttca tctttcgcgt cgttccgcca cggagccggc
    45121 gctcggggcg gtgatggtgc gcgcggcgga accgggtgga cgagtgggac gcatctggtg
    45181 tggcctgacc aataccgagc cctcggacgt gggcctggac cagttggggc attggctggt
    45241 gtcgctggat caggcaaaac aggcattccg ccatatgggc gcgtgttggc gcctgctggc
    45301 gcaatccggt tcgccgtcga aacctgccct gcatcaggcg ctgacggcgg cgcagatggg
    45361 gctcgcggtc tggcaggccg gcaaggacga ggccttgagt tcggtgttgc gcactctgac
    45421 ccctgggcga tttgcccccc acgccgcctt atcgcggttg ttcacgttca aggaagtcct
    45481 ggaacgggaa gagggcgcat ccgcgtaatg aagggatgtt ggcgaacgac acgcgcaccg
    45541 ggcgtcaggc gatggcgcgg cctttggcgt tgtacaggtt gctggtgccg gcgaggttgc
    45601 gtagcgtttg gatggcgcgt tgcgtgtgcg ttaggtagat ttcgatggcg gcgccgttct
    45661 ccaggttcaa ccggcgcgct tcagcggcca gtgcctgcaa ttcgtggcac agcggcgcaa
    45721 gagccggatc gtttcggctg ctcgcgtcca gaccctgctt tccaattggc aaaccctgtt
    45781 gggccagcag ggcatcgcgt tggttcgaca cctgggcgag gctttcggcg cattgattct
    45841 tcgcgtgggt ggaggcctgc agcgccgtgt tgtcatccgg atgctccagc gcctgggcct
    45901 cgtcgtgcag aacatcgatg aagtggcgta ccagcgcgca ttcctgttcg aggcagccgc
    45961 gcagatcgat ggtgtgtggg ggcatcgtgg cgtatccgga agtggaacga ttatttgagc
    46021 agatcgcgcg cgctggcgat gattttgtcg gcgacgcgcc ccgaatcgag ggtcagttgc
    46081 ccggacgcga tggcggcgcg caaggcgttg acacgttcga cgtcgacatc gttgtcgctg
    46141 ttttgaagat tcgacagttg ccgtgccgcc ggactgagtt cgacggcgct ccggttgttg
    46201 gcgcccgccg ccggtgcccc ggtggaagcc ggccggttgg ccgcctgaac ccccgtgagc
    46261 ggggtctggg aggatgcggt ctggattttc aagatgggct ccggagggat cggcgattgg
    46321 agagtcgagg ttcttcgatc agtataaagt gtaacggcgg cgcggaagga aactttaccc
    46381 cccaggaaga gcccggacca cggcctgctc gacagatgcg cacggagggc ggttgcgacg
    46441 cttgatcaca tggccacccg cacggtatgg gcatcgacca ccgtcgcgct gatgatgcgc
    46501 cccgacgacg tgcggacttg tatctgcgcg ccgggtgggc cgttttcgag tgctttgccc
    46561 gatccggtgg cgatgaagcc cacgccttgc gcgacagtgc ggacgaactg gccgcgctgg
    46621 atcgactccg gactgcgcag cgcgcgtgcc cggatggggc ggccgacggg gatgcgatac
    46681 gcggcgatgc gccccacgat atcttgtggg tcggtgaccg tgcccgccgg caagcgcaac
    46741 aaatcgcctt gtcggggggt caggtcaccg ggaacgaccg tggcatccgc ggggatgggt
    46801 agcccggcca catagtatgt gcccatgatg accacgctgg cctgcgcaaa agtcgtccag
    46861 ggttcgggag cgaggcagcg gacacccacg gacatgcggg attccaggcg ttgcccgcca
    46921 cgcaggaaca cctgcgggtt atcacatgaa ggtagccggc tggtgtcggg gggatcgacc
    46981 gtgatctggg cagttccggg ccaggcgcgt gcctgttgca tcaggaaatg cctgacgctg
    47041 cgtgccaccg cgtctgggtt ctggaacgtg gacgccatgg tggcgctggc cgtgccggaa
    47101 agaccgaaca agatgagcag tgggagagtg cggagcagtg ccatgcttga attctatagc
    47161 tggcccgtcg cacgcataaa cggaactgac cggcaattgg cggcttgttt catattttat
    47221 ggcgcgcgtg cccaccgtac catgtcattc gaaaatggcg tcgagcgcgg cgggaccgga
    47281 tcctatggcg gttggcggca gacgtaaacc cagtccacag agagcgagtc atggccgacc
    47341 ccattggcag cgatttgagt ttctatcagg cggcgatcgc cttgcggcag cggcggcagg
    47401 aagtgctggc gtcgaacatc gcgaac
//
LOCUS       DASTNK010000018         4903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_383086, whole genome shotgun sequence.
ACCESSION   DASTNK010000018 DASTNK010000000
VERSION     DASTNK010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383086"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1049
                     /locus_tag="VFR20_01425"
     CDS             96..1049
                     /locus_tag="VFR20_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0229207.1"
                     /translation="MAAHNLDINALRTFETGVALGTFAKASDRIGRSPAAVSAQLRKL
                     EQQAGTELFRKAGRGLALTEAGETLLSYARQILAANDAALNALHSTQARGKVCLGLQE
                     DLGEWVLTDVLGHFARAHPGMRIETRVCRNAELMDRVAAGELDLAVVWGNGGAAPWRE
                     TLARLPMVWIGGLNGIGRLNHAAAPGRAVGAARNTATNRADESDEARPLALVAFDGAC
                     LFKTAALEALNQAGIAWDVTFTSSSLNGLLAAVAAGLGVTIRTPIGLPPRVAVLQPSA
                     SGLPGLPKVDLTMIATRSEQSPAVAFLADALRTAIHGRLPH"
     gene            complement(1167..1535)
                     /locus_tag="VFR20_01430"
     CDS             complement(1167..1535)
                     /locus_tag="VFR20_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEU0229208.1"
                     /translation="MKAIAWLMLVAAVLPFAATIIAKAGGAGFDNNNPRPWLARQEGW
                     RARANAAQSNLFEALPFFYGAVLFALFNHADVNSVAALMLAWVVLRFIYVGVYIGGRG
                     AVRSVIWAVCLILNISILFT"
     gene            1722..2726
                     /locus_tag="VFR20_01435"
     CDS             1722..2726
                     /locus_tag="VFR20_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU0229209.1"
                     /translation="MSNIKIVAKLAGCSIATVSRCFNAPDLVRPATRARVMEVARQQK
                     FRPSIIGQQLRRNRTGLVGVMLPSLANPIFADCIGGIEEVLDQHNKRVLLTTTQYDST
                     KEEQLIEILLRQKVDGLLLTVANTHDCRALAMLAEEKVPYILMYNHAPGYATVSVDDR
                     IAARDAVRHLVRAGHRRIAMLAGYLTASDRSALRHQGYQDALREAGLKPLPLVEADFD
                     AHTLDTQLQHWFRTMANRPTAIFCSTDLLALGVIKSLRERHLRIPDDVSVMGFDGLEY
                     GQLIEPTLATIRQPNEDIGRQAARRLLAQIDGEPMQPEPLFLPHTLLTGRSVNQGGKP
                     "
     gene            2750..3739
                     /locus_tag="VFR20_01440"
     CDS             2750..3739
                     /locus_tag="VFR20_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010092742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0229210.1"
                     /translation="MGLLALTAGLYTGAAQAENLAICYNCPPQWADWAGEVADIKKDT
                     GITVPLDNKNSGQALAQLITEKASPVADVVYLGISSAIAAMKYNVLGTYKPKYWDQIP
                     ADLKDPNGHWFAIHSGTIGLFVNKAALEGKPVPQSWADLLKPEYRGLVGYLDPSSAFV
                     GYAGAIAINQAMGGTLNNFKPAIEYFKKLKKNHPIVPKQTSYARVLSGEIPILIDFDF
                     NAYRAKYKDHADVVFVIPKEGTVSFPYVMAMVKGAPHPENAHKVLDYVLSDKGQEHWA
                     SAFLRPVRNVKIPADIASKFLPASDYARAKTVDYNKVAAEQEAFQRMYLDQVQ"
     gene            3772..4596
                     /locus_tag="VFR20_01445"
     CDS             3772..4596
                     /locus_tag="VFR20_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0229211.1"
                     /translation="MRDLTLPAHWRTLLLLPALIIFTAFWLIPIAVLVRISAGGNFLH
                     TYAAILSDSRYLTSLWQTVALSLMVTVVTIILSVIAGLFLTRNEFPGKRLLVSLLTLP
                     LAFPGVVVGFMVIMLAGRQGIIGDISHALIGQKLVFAYSMSGLFLGYLYFSIPKIVLS
                     VMAAAQSMDPALTEAAHSLGAGPWAVMRDITLPALRPTLIASGAICFAISIGAFGTAF
                     TLATDINVLPMTIYTEFTLNANLVTAAALSVILGVVTWAILMLARNATGTTMVAGG"
     gene            4593..>4903
                     /locus_tag="VFR20_01450"
     CDS             4593..>4903
                     /locus_tag="VFR20_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0229212.1"
                     /translation="MMTLKQRLLFIAQLAITLLICAFLFVPVVVSAITGFSVSYFRGL
                     SAGLTLQWVTQVLTDYHGAIVNSLLVAAATLTIVLLVGVPAGYVLARWESRMARLIEE
                     L"
BASE COUNT          944 a         1722 c         1386 g          851 t
ORIGIN      
        1 cctgaaaaaa cgattgatgc aggaatctta agtcgctata ttgttctgat aaatcaaatt
       61 gtttaatact aacaattaaa tttaatggaa ttaatatggc cgcgcacaac ctcgacatca
      121 acgccttgcg caccttcgaa accggcgtgg cgctgggaac gttcgccaaa gcctccgacc
      181 gcattggccg ttcgccggcg gcggtcagcg cccagctaag gaagctcgag cagcaagccg
      241 gcacggaact cttccgcaag gcggggcgcg ggctggcgct caccgaagcg ggtgaaaccc
      301 tgctcagtta cgctcgccag atcctggcgg ccaacgatgc cgcgctcaac gcgctgcata
      361 gcacccaggc gcgcggcaag gtctgcctgg ggttgcagga agacctgggg gaatgggtgc
      421 tcaccgacgt tctgggccat ttcgcgcgcg cccatccggg catgcgcatc gaaacccggg
      481 tatgccgcaa tgccgaattg atggaccgtg tcgccgccgg cgaactggat ctggccgtgg
      541 tctggggcaa tggcggcgcg gccccgtggc gcgaaacact ggcgcgcctg cccatggtgt
      601 ggataggcgg cttgaacgga atcggccgcc tgaaccacgc cgcggcgcct gggcgcgcag
      661 tgggcgcggc gcgcaacacc gcaacgaatc gcgccgatga atccgacgaa gcccggccgc
      721 tggcactggt ggcgttcgac ggcgcctgcc tgttcaagac cgccgccctg gaagccctga
      781 accaggccgg catcgcctgg gacgtgacct tcaccagttc cagcctgaac ggcctgctgg
      841 ccgccgtggc tgcgggcctg ggggtgacca tacgcacacc catcggcctg cccccgcgcg
      901 tggctgtgct gcagccgtcg gccagcggct tgcccggctt gcccaaggtg gatctcacca
      961 tgatcgccac ccggtcagag cagtcgcccg ccgtggcctt cctggcggat gccctgcgca
     1021 cagccatcca cggacgtctt ccccactgac ggcgccaagc gcggccgtgt cctcggaaac
     1081 cactgctccg gcctcacgcc aaccgccacg gcgcccctac ctaagtcaag cgccttgcgt
     1141 aaagcgtgcc aagcgcccac ttgtcgctac gtgaacagaa tcgagatatt cagaatcagg
     1201 cacaccgccc agatcactga gcgcaccgcc ccccgcccgc cgatatacac cccaacataa
     1261 atgaagcgca gcaccaccca ggccagcatc aaggcggcca cgctgttgac atcggcatgg
     1321 ttgaacaacg cgaacagcac cgcgccgtag aaaaagggca gggcctcgaa cagattcgac
     1381 tgggcggcgt tcgcccgggc gcgccaacct tcctggcgcg cgagccacgg ccggggatta
     1441 ttgttgtcga accccgcgcc gccggccttg gcgataatgg tcgccgcgaa aggcagcacc
     1501 gccgccacca gcatcaacca cgcaattgct ttcataacta ccacctccgg ggtttgtcgc
     1561 catacccatc acgatgcgac aagcattcat gacagtatgg acgcatccgc catagctcca
     1621 ttctaagtag tatccctagg cactgtgaag tccacattgt tataaaattc acttcaaatg
     1681 aaaacgtttt cattttagtc ggcgacattc tggaccaaac catgtccaac atcaaaatcg
     1741 tggcgaaact ggcgggctgt tccatcgcga ccgtctcgcg ctgcttcaac gcgcccgacc
     1801 tggtccgccc cgccacgcgc gcccgggtca tggaagtcgc ccgccagcag aaattccggc
     1861 ccagcatcat cgggcagcaa ttgcgccgca accgcaccgg cctggtgggc gtcatgctgc
     1921 cgtccctggc aaaccccatt ttcgcggact gcatcggcgg catcgaagaa gtgctcgacc
     1981 agcacaacaa gcgtgtgctg ctcaccacca cccagtacga ttccaccaag gaagaacagc
     2041 tcatcgagat ccttctgcgc cagaaggtcg acggtctgct gctcactgtc gccaacacac
     2101 acgattgccg cgcgctggcc atgctggccg aggaaaaagt cccgtacatc ctcatgtaca
     2161 accacgcgcc cggctacgcc acggtgtccg tcgacgaccg catcgcggca cgcgacgcgg
     2221 tgcgccacct ggtgcgggcc gggcaccgcc gcatcgccat gctggccggc tatctcaccg
     2281 cgtccgaccg ctccgccctg cgccatcagg gctaccaaga cgccctgcgc gaagccggcc
     2341 tcaagcctct tcccctggtc gaagcagact tcgacgccca caccctcgac acacaactgc
     2401 aacactggtt ccgtaccatg gcgaaccgcc caacggccat tttctgcagc accgacctgc
     2461 tggccctggg tgtcatcaaa tccctgcgcg agcgccacct gcgcattccc gacgacgtct
     2521 ccgtcatggg gttcgacggt ctggaatacg gccagctcat cgaacccacg ctggccacca
     2581 tccgccagcc caatgaagac atcggccgtc aggcagcccg acggctgctc gcgcaaatcg
     2641 acggagaacc catgcaaccc gaaccgctgt tccttcccca tacactttta accggacgct
     2701 cggtcaacca aggaggcaaa ccatgatgaa gattccattc cgtgcgctga tggggctgct
     2761 ggccctgacc gccgggctgt acaccggcgc ggcgcaggcc gagaacctgg ccatctgcta
     2821 caactgtccg ccgcaatggg cggattgggc cggcgaagtc gccgacatca agaaggacac
     2881 cggcatcacg gtgccgctgg acaacaagaa ctcgggccag gcgctggcgc aactcatcac
     2941 cgagaaagcc agcccggtgg ccgacgtggt gtacctgggc atcagttccg ccatcgccgc
     3001 catgaagtac aacgtgctgg gcacctacaa gcccaagtac tgggatcaaa tccccgccga
     3061 cctgaaagac cccaacggcc attggttcgc catccattcc ggcaccatcg gcctgttcgt
     3121 caacaaggcc gcgctggaag gcaagccggt gccgcaatcg tgggccgacc tgctcaagcc
     3181 cgaataccgc ggactggtcg gctacctgga ccccagcagc gcgttcgtgg gctacgccgg
     3241 cgccatcgcc atcaaccagg ccatgggcgg cacgctgaac aacttcaagc cggccatcga
     3301 atacttcaag aagctgaaga agaaccatcc catcgtgccc aagcaaacgt cgtacgcgcg
     3361 cgtgctgtcg ggtgaaatcc ccatcctcat cgacttcgac ttcaacgcct accgggccaa
     3421 atacaaagac cacgccgacg tcgtcttcgt cattcccaag gaaggcacgg tgtcgttccc
     3481 gtacgtgatg gccatggtca agggcgcgcc ccaccctgag aacgcgcaca aggtactcga
     3541 ctacgtgctg tctgacaaag gccaggaaca ctgggccagc gccttcctgc ggcccgtgcg
     3601 caacgtgaag atccccgccg acatcgcctc gaagttcctg ccggcgtccg actacgcccg
     3661 cgccaagacg gtggactaca acaaggtcgc ggccgaacaa gaagcgttcc agcgcatgta
     3721 cctggaccag gtgcagtgac cgcctcaccc acgacggact cttgacgaat catgcgtgac
     3781 ctgaccctgc cggcgcattg gcgcaccttg ctgctgttgc cggcgctgat catcttcacc
     3841 gcgttctggc tcatccccat cgcggtgctg gtgcgcatca gcgccggcgg caacttcctg
     3901 cacacgtacg ccgccattct cagcgactcg cgctacctca ccagcctgtg gcagacggtc
     3961 gcgctgtcgc tgatggtcac cgtggtcacc atcatcctgt ccgtcatcgc gggtttgttt
     4021 ttgacccgca atgaattccc cggcaagcgt ctgctggtgt cgctgctgac gctgcccctg
     4081 gccttccccg gcgtggtggt cggcttcatg gtcatcatgc tggccgggcg ccagggcatc
     4141 atcggcgaca tcagccatgc actgatcggc cagaagctgg tgttcgccta ttccatgtcc
     4201 ggcctgttcc tgggctatct gtatttttcc atccccaaga tcgtgctctc ggtgatggcg
     4261 gccgcgcagt ccatggatcc cgcgctgacc gaagccgccc attccctggg cgccgggccc
     4321 tgggccgtga tgcgcgacat cacgctgccg gccctgcggc ccacgctcat cgcctcgggc
     4381 gccatctgct tcgccatttc catcggcgcg ttcggcaccg ccttcaccct ggccaccgac
     4441 atcaatgtgc tgcccatgac catctacacc gagttcaccc tgaacgccaa cctggtcacg
     4501 gccgcagcgc tcagcgtgat actgggtgtg gtcacctggg ccatcctcat gctggcgcgc
     4561 aacgccaccg gcaccaccat ggtcgcgggg ggatgatgac actcaagcag cgtttgctct
     4621 tcatcgcgca actggcgatc acattgctga tctgcgcctt tctgttcgtg cccgtggtgg
     4681 tctcggccat caccgggttc tcggtcagct acttccgcgg cttgtcggcg gggctgacat
     4741 tgcagtgggt cacacaggtc ctcaccgatt accacggcgc catcgtcaat tcactgctcg
     4801 tggccgccgc cacactgacc atcgtgctgc tggtgggcgt gccggccggc tacgtgctgg
     4861 cgcgctggga aagccgcatg gcgcgcctga tcgaggaact cat
//
LOCUS       DASTNK010000019        19594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_754047, whole genome shotgun sequence.
ACCESSION   DASTNK010000019 DASTNK010000000
VERSION     DASTNK010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 19594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19594
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754047"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VFR20_01455"
     CDS             complement(<1..470)
                     /locus_tag="VFR20_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HEU0229213.1"
                     /translation="MAERSFKKEVEQLRIGAGQEFRGEGILAVTKALLQCGVGYVGGY
                     QGSPISHLMDVLADAQDILEELGVHFEASASEATAAATLAASVMYPIRGAVTWKSTVG
                     TNVASDALANLASGGVTGGALVIVGEDYGEGSSIMQERTHAFAMKSQIWLLDPKP"
     gene            811..1248
                     /locus_tag="VFR20_01460"
     CDS             811..1248
                     /locus_tag="VFR20_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0229214.1"
                     /translation="MAGNMDQDRFVYRYLASMLALASHRISAEFHVEVRKAGLTVTEW
                     RILASLFEGEGETVGELARLAITKQPTLSKVLPRLEAQGLITLGTTRADRRQTLVKIS
                     PKGARLIGGLCESAMAHQRRVLARLDPDHADRLVGMLRDIIAD"
     gene            1412..2299
                     /gene="rpoH"
                     /locus_tag="VFR20_01465"
     CDS             1412..2299
                     /gene="rpoH"
                     /locus_tag="VFR20_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEU0229215.1"
                     /translation="MTQRSQALALPQPNALALAISNPGALGTIEAYIGAVNRIPMLSA
                     EREAELGERVRDHMDLDAARELIMSHLRLVVSVARHYLGYGLAHADLIQEGNIGLMKA
                     VKRFDPARGVRLVSFAVHWIKAEIHEYIVRNWRLVKVATTKAQRKLFFNLRRMRPDGQ
                     TLDSTQVDDIARELNVRPEDVSEMEVRLSGRELALDAQPDEDDTFAPIAYLSDEGRQE
                     PSQVLERRAHDTLQGSGLEQALEALDPRSRRIVQARWLRDEGGATLHELAAEFGVSAE
                     RIRQIEAAALKKMRGALAG"
     gene            2714..5386
                     /locus_tag="VFR20_01470"
     CDS             2714..5386
                     /locus_tag="VFR20_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU0229216.1"
                     /translation="MLIGQLVFGLAGLLALVSFMPPLAGRLRVPYSVLLAVVGFILGI
                     IIHVHGWAPPVMGDFLDSLQHFEVSSETFLFVFLPVLLFETALAMNVRRLMDDLGPIL
                     MMAIVAVLVSTVAVGYVLSAASSYGLVVCLMLGAIVATTDPVAVVGVFHEVGAPKRLN
                     TLVEGEALFNDAASISLYSVLLAMLLGGGAVSAGSVLSTFLINFLGGGVAGFLMGRLA
                     CWLVMWLRGWPTAEITLTVAFAYLTFFLSEHYLGVSGVVATVIAGLVVGSTGRTRMSP
                     STFEQLRGAWSEFGFWANSLIFVFAAMLIPRMMADVTWHEVALVILLFFVALIARAVM
                     VFGVLPLLGRTRFGTRVSLSYQVVIAWGGLRGAVSLALALAVTEQVLVPYQARQFVAV
                     ATTSFVLMTLFINGMTLRPLIKRLRLDQLSPIERTIRNQVQVMALSDLQDKTAELASR
                     EHIGPEVSARIHVVFDASLASVADAQVRQLSHDERVTVGLMMMAAREEEMFFDVLKAR
                     IVDWRMAESLLARAERMSDAVRNGGLAGFESAIAADLRYSLGFRAALRTHYVFGFQGW
                     LARELANRFTNLMSKRSVAQRLIQFAQTEIRPLLGSEATETIIGAHQRRLGQLENALQ
                     ALSLQYPLFASWLQESYLGRMARALERLRYRDMLSQFLISNEVYADLVDQIEQRWRYV
                     DERPALDIALSASDLIKRVPLFEGLSADSLKAIEKLLKPRLMLPDQRIDIQLGRTRAM
                     YFVASGAVSLPLPDGTTAELGSGEFFGEIALLSGKDFATEATSLGYSRLLRLLARDFD
                     ALLARDAVLREKIEKVAKQRLRALEVWQEFQSGKRQYEPLPVSPEREAENGGPMGEVD
                     AHANAGRVASAPGGEAAQSGEHVEPGDRGPTAAT"
     gene            complement(5370..5978)
                     /locus_tag="VFR20_01475"
     CDS             complement(5370..5978)
                     /locus_tag="VFR20_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEU0229217.1"
                     /translation="MNSFAFSARRRAVTAALAAGMLSVTLAGCGNDKPAFTGSDVTGT
                     HLGRDMSMTDATGHVRTLADYKGKVVVVYFGYTHCPDVCPTSMAELAQTMALLKGDAK
                     KTQVIMITVDPARDTPAVVDHYAKAFDPSFVGLGGTPEQLAKTAKSFKTYYAKESSKI
                     PGQYAMNHSSMFYIIDQKGETRVILNSTVPPDVVAHDIRLLL"
     gene            complement(5980..6873)
                     /gene="cyoE"
                     /locus_tag="VFR20_01480"
     CDS             complement(5980..6873)
                     /gene="cyoE"
                     /locus_tag="VFR20_01480"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEU0229218.1"
                     /translation="MTSLSAPSPGLLRQYLVLTKPRVTQLAVFCAVIGMFLASPGVPD
                     LRAVVAGTVGIWLLAAAAFSINCLIEREVDARMLRTARRATARGTISNAQVLSFSGVL
                     GGVGMLVLYNLVNPLTMWLTFATFVGYAVIYTAILKPLTPQNIVIGGLSGAMPPALGW
                     AAISNSVPAEAWLLVLIIFIWTPPHFWSLALYRNNDYIKSGLPMLPVTHGQQFTRLHI
                     LLYSLILFAASLMPFAIRMSGFAYLLAAVVLSGLFIHYAWRLYREYSDDLARKLFRYS
                     ILYLSLLFGALLVDHWLRMMF"
     gene            complement(6870..7883)
                     /locus_tag="VFR20_01485"
     CDS             complement(6870..7883)
                     /locus_tag="VFR20_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEU0229219.1"
                     /translation="MDRIKARYRKLVFTTWFLTLDLIMFGAFVRLTDSGLGCPDWPGC
                     YGHVTPIGAASHIEGALKAMPYGAVSFSKAWIEMVHRYAGSILGLLIIAIVVMAWRYR
                     RQLGNTPYLATVALVAVCVQGAFGAWTVTHRLMPVIVTTHLLLGMSLLALMTWLAARE
                     KSHLPVQPAMGSALPWMGVGLALLFTQIALGGWVSTNYAALACMDFPTCHGQWLPTMD
                     FRDGYSLIRALGMLPSGAMISQAALTAIHWTHRNFAFIVFIYLGVMGFRLRRDAGLRG
                     PATLMLALLLAQLCTGLTTIFFQWPLAVAVLHNGGAAGLVLATVTLLVRLSRAKNGAK
                     IPS"
     gene            complement(7892..8482)
                     /locus_tag="VFR20_01490"
     CDS             complement(7892..8482)
                     /locus_tag="VFR20_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229220.1"
                     /translation="MFPLYAILAVCIAPVVLAWLAYYFPSLGLRPSGTTNYGTFVQPQ
                     RPIPPASALALTTLDGKPFDLSSLKGKWILLTADQGACPKACVRKLFILRNAHASQGK
                     NVERLTRVWFVTDDAPVPADVLKAYGGTHIVRAKPAQLAAFLAPRAGTTREAALRDPM
                     WVIDPLGHLMLRFPANADPISVRDDLKKLLHNSQIG"
     gene            complement(8578..9369)
                     /locus_tag="VFR20_01495"
     CDS             complement(8578..9369)
                     /locus_tag="VFR20_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEU0229221.1"
                     /translation="MARPHSPIRTLAALFLLGVMIAVFIPLGNWQLRRAELRKSTMHA
                     IEAGEHGAPLSIAADSPRTTLKEWHPATARGVWLDKFTVLLNRNDKGRPGYWVATPLL
                     IDAASHTAVLVLRGWLPFVGAGKTPDVAPAAPGPQDVAGTLVSRVPRLFELWRFSGAS
                     PDKLPTTFPAPGGTLPQLQNLNLHDYSRATGLKFVPAVLEQTSPGGDSLARDWPRPSI
                     DYARNNGYALQWFSFAAIAAGAWLVVAWRALRRWRARHSSPQPSA"
     gene            9403..9609
                     /locus_tag="VFR20_01500"
     CDS             9403..9609
                     /locus_tag="VFR20_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEU0229222.1"
                     /translation="MHLIVIVAFLGIVASLGSALISLMRDKGTSNRTVNALTVRVGLS
                     IALFLFVLFAHHMGWIQATGLTQH"
     gene            complement(9665..10540)
                     /locus_tag="VFR20_01505"
     CDS             complement(9665..10540)
                     /locus_tag="VFR20_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEU0229223.1"
                     /translation="MSASHSAQGTTAPYYYVPAHSSHPVQASISMLMVLIGAALWING
                     FEPGPWLFLLGVICMLTTLFFWFKDAIGESEGGMNSGKVDHSYRWSMSWFIFSEVMFF
                     GAFFGSLWYTREVSLPWLTNLDHQTLLWPHFADVWPNFGPAGTIEPFKAVGPFWLPTI
                     NTALLLTSGLTITIAHHALRANHRAKTIFWMIATVILGFAFVACQAHEYYDSYTELNL
                     KFDSGAFGSLFYMLTGFHGFHVILGATMLTIITLRLIRGHFTPNHHFGFEGASWYWHF
                     VDVVWLGLYLFVYWL"
     gene            complement(10602..10826)
                     /locus_tag="VFR20_01510"
     CDS             complement(10602..10826)
                     /locus_tag="VFR20_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HEU0229224.1"
                     /translation="MDDDNPRPQEDRASLLQTLKAVSWGFLGVRRRQDQEKDAKGISP
                     GYLIIIGLLLTLVFVLVLVTIARLFVANLT"
     gene            complement(10845..10949)
                     /locus_tag="VFR20_01515"
     CDS             complement(10845..10949)
                     /locus_tag="VFR20_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase small assembly protein"
                     /protein_id="HEU0229225.1"
                     /translation="MTPEQRRRSLRTGIILLIFVIAIMAWTFWSGFKA"
     gene            complement(10953..12554)
                     /gene="ctaD"
                     /locus_tag="VFR20_01520"
     CDS             complement(10953..12554)
                     /gene="ctaD"
                     /locus_tag="VFR20_01520"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEU0229226.1"
                     /translation="MSSVSIDHVPPGTGHDDHTHAVPYGWRRWLFATNHKDIGTMYLI
                     FSFCMLLEGGVLALLIRTELFEPGIQFFQPELFNQFTSMHGLIMIFGAIMPAFVGFAN
                     WMIPMQIGASDMAFARMNNFSFWLLPVGATLMTVSFFVPGGAQAAGWTMYAPLSLQMG
                     PGMDFTIFAIHILGASSIMGAINIIVTILNLRAPGMTLMKMPMFCWCWLVTAYLLLAV
                     MPVLAASVTMLLTDRHFGTTFFNAAGGGDPVLYQHVFWFFGHPEVYIMILPAFGMISE
                     VIPVFARKKLFGYTSMVYAVCAIAILSFLVWAHHMFATGMPVTAQLFFMYATMLISIP
                     TGVKIFNWVATMWRGSLSFETPMLFAIGFIFVFMIGGFTGLVLALAPVDIQLTDTFYV
                     VGHFHYVLVASALFAMFSATYYWLPKWSGRMYDEKLGKWHFWMSMISFNVGFFPMHFL
                     GLAGMPRRVADYAPQFTDFNQIATIGAFWFGLSQIIFIYVVAKAVSGRGERASANPWG
                     AEEGLEWTVPSPAPHHTFVTPPIVN"
     gene            complement(12650..13810)
                     /gene="coxB"
                     /locus_tag="VFR20_01525"
     CDS             complement(12650..13810)
                     /gene="coxB"
                     /locus_tag="VFR20_01525"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEU0229227.1"
                     /translation="MKKLRGILGTGAWLWMAAASAKIENMPGGPQVNQLDLHKGVTTI
                     SQRIWDLNWMIMIICVIIFVGVVGVMFYSIIMHRKSRGHVASKFHEHTALEVAWTVIP
                     FLIIISMAIPATKTVVAMKDTSSPDLSIKVTGYQWKWGYDYLDGAAAGVHFLSNLKTP
                     MSQIDGKAPKDNFYLLEVDKPLVVPVNKKVRVIVTADDVIHSWSVPDFGVKQDAIPGF
                     LRDTWFRATKIGTYRGACSELCGKNHAYMPIVVKVVSDADYVKWAKKEKGAMLASSDD
                     PNKKYSKEELVARGKQVFTSNCVSCHQANGKGIPGTFPALDGDKKFVLAPMKGQILTV
                     LNGHPGTAMPAWRSQLDDVQVAAVITYTRNAWDNAGKGPDPVVQPSDVKALR"
     gene            14120..14950
                     /locus_tag="VFR20_01530"
     CDS             14120..14950
                     /locus_tag="VFR20_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4743 domain-containing protein"
                     /protein_id="HEU0229228.1"
                     /translation="MTSPGAGSLPAAIEVRRVALLRSLQQLPSNGSRPLTVSARVAGW
                     VTARATASLRGLPGVRIEDEAVHITASTSPRLSLNAVLARVAQVLDEAGCIRAWRDEM
                     LDVVGEGRRLAAIERGAMRPLGLLTRAVHLNAWTPQGELWIARRALTKSTDPGKWDTL
                     VGGLVGAGEALDTALVREAAEEAGLTPEAMAGHSPLRIILRMHKRLPEGYQVEDLFIS
                     ECVLDDGVALANQDGEVSEFRRAGVEEVLSLMRARAFTAEAELVLLEGIRGRLGVATN
                     "
     gene            complement(15032..15313)
                     /locus_tag="VFR20_01535"
     CDS             complement(15032..15313)
                     /locus_tag="VFR20_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3717 domain-containing protein"
                     /protein_id="HEU0229229.1"
                     /translation="MKPSYTLAELESAINYWRARSPSTGEESALCAEAAALAEPYALL
                     IFNARHDVSWNNLARDAQEALRGWVASQPVESGKASAQGNPPPDSPHAR"
     gene            15397..16095
                     /locus_tag="VFR20_01540"
     CDS             15397..16095
                     /locus_tag="VFR20_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HEU0229230.1"
                     /translation="MPAESPNPFVLPGLGQTGDQAQNPLLAGMEMMRQAWQGLASAGG
                     LSQSAMAAPMTLEELDKRIADLRAVENWLRMNLSMLTSAIQGLEVQRATISTLRSFVE
                     SAAGAGQAAAGQSPLEAALGIHKPDAPAQAGKKAKGAAADGASSGASAMPSSAQAWWN
                     MLQGQFDTLAAATAATLRGADDAKTAAQPGAGAGAESAVKSTPSKTAKGRKPSSGTRG
                     RAATPRKTPGGAGE"
     gene            16150..17520
                     /gene="glmU"
                     /locus_tag="VFR20_01545"
     CDS             16150..17520
                     /gene="glmU"
                     /locus_tag="VFR20_01545"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU0229231.1"
                     /translation="MLNIVILAAGLGKRMQSDLPKVLHPVAGKSMLAHVLDNARELNP
                     DQIVIVVGHGADRVRQAFTDEKDVAFALQVPQQGTGHAVGLAAPLLRDDGASGTTLVL
                     YGDVPLVQADTMKRLIAARAQGMAVLTQRLDDPSGYGRIVRDQAGHIRRIVEHKDASP
                     AEQVINEVNTGILAVPTAWLKAALPRIGNDNAQQEYYLTDLVGLAVGDGVAVNSAEPG
                     ADWETLGVNSRIQQAQVERVWQGEQARRLLEQGVTLADPARLDVRGSLACGRDVYIDV
                     GCVFEGDVSLADGVRIGPFCVLKNAEVGAGTQIDAYTHVQEAVIADDARIGPYARLRP
                     GADIGAQAHVGNFVEIKKSTLGRGSKANHLTYIGDATIGQRVNVGAGTITCNYDGVNK
                     FRTIIEDDAFIGSDTQLVAPVTVGRGATLGAGTTLTRDAPADQLTLSRPTQKSIPGWK
                     RPVRKG"
     gene            17549..18637
                     /locus_tag="VFR20_01550"
     CDS             17549..18637
                     /locus_tag="VFR20_01550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0229232.1"
                     /translation="MRILELNFERGWRGGERQTLYCMRQFRRAGHDVSLMARRGGKIA
                     AVARAEGFNVIEQAGPAGQAAYLMASGNHFDIIHAQTANTITWAALTRWRHRRPVVFS
                     RRTDFHVRHDARTRFKWRHVDWFVAISESAAAEPRRLGLAPSIIRSAIEPVSVDDARV
                     ARFVEEFGLHGKRLIGTSAALVDDKDPLTMIRAVHRLAGQRDDFVFVHLGGGGDAEPA
                     ARALVDELGLQKVYLFAGFQSDVESLYSIMDVFAMSSVEEALGSSVLDAFLQRVPVVS
                     TDAGGLKESLADGRGVLCRARDHEAMAEGMRRMLDDAGFRDAAVERAYDYVRREHDVV
                     EMGRRYLEGFERLGSKTERRRGPSGIKR"
     gene            18642..>19594
                     /locus_tag="VFR20_01555"
     CDS             18642..>19594
                     /locus_tag="VFR20_01555"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019248572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEU0229233.1"
                     /translation="MKITVFGSGYVGLVTGACLADGGHHVLCMDVDQAKVDGLRRGVV
                     PIYEPGLEGLIQRNMADGRLGFTSDPAEAVAFGVLQFIAVGTPPDEDGSADLQHVLAV
                     ARTIGMHMADHKVVIDKSTVPVGTADKVRATVAEALAARGLSIDFDVCSNPEFLKEGA
                     GIEDFTRGARIVVGTDSERVKALMTECYAPYNRNHDKFMFMDVRAAELTKYAANAMLA
                     TKISFMNEMANLAERLGADIEQVRRGIGSDPRIGYHFIYPGCGYGGSCFTKDVQALTQ
                     SAESTGYQAELLRVVEAVNRRQKQTLLQKLDAAFNDEVQGKT"
BASE COUNT         3600 a         5906 c         6566 g         3522 t
ORIGIN      
        1 ggcttggggt cgagcagcca gatctgggat ttcatggcga acgcgtgcgt gcgttcctgc
       61 atgatggagg acccttcgcc atagtcttcg cccacgatga ccagcgcccc gcccgtgacg
      121 ccgccagaag ccagattggc cagggcgtcg gaggccacgt tggtgccgac cgtggatttc
      181 caggtgacgg cgccgcggat ggggtacatg accgacgccg ccaatgtggc ggccgcggtg
      241 gcttcggaag cgctggcctc gaagtgcacg cccaattctt cgagaatgtc ctgcgcatcg
      301 gccaggacgt ccatgaggtg cgagatcggc gaaccctggt aaccccccac gtagccgacg
      361 ccgcactgca acagcgcttt ggtgacggcc aatatgcctt cgccgcggaa ttcctggccc
      421 gcgccgatgc gcaactgttc gacttccttc ttgaatgagc gttccgccat gatgtctccc
      481 cggatcaatc gccggacgat gggtgccgga aggcgcgttg ccgtggtcgg gacgaccagc
      541 cgaggccttt ctttttccag gtactttaag attcaataat caagctgtca ataaaaatat
      601 gaaaattcat ataatttcca aagttaatag gaataacagt aggttggaag ttggtgtttt
      661 ccattagacc gcgctgtggc gaggccaggg cagccgcaaa ttcctgttgt tggcgggcgg
      721 tttggtgctc tactatcggg tgaccggagc actcgcttcg ggcgacggat ggcgcatagc
      781 cgccgcatac ctggaacagg aacccaccgg atggccggca acatggatca ggatcgcttc
      841 gtttatcgtt atctggcctc gatgctggcg ctggccagcc atcgcatctc ggcggaattc
      901 cacgtcgagg tccggaaggc cgggctgacc gtgaccgaat ggcgcattct cgccagcttg
      961 ttcgagggcg agggtgaaac cgtgggtgag ctggcccgcc tggccatcac caagcaaccc
     1021 acgctcagca aggtgctgcc gcgtctcgag gcccaaggcc tcatcacgct gggcacgacc
     1081 cgcgccgacc gccgccagac cctggtgaag atctccccca agggcgcgag gctgatcggc
     1141 ggcctgtgcg agagcgccat ggcgcatcag cgccgtgtgc tggcccggct cgacccagac
     1201 catgccgacc ggttggtcgg catgctgcgt gacatcatcg ccgattagag cctgttcgcg
     1261 ggcggagacc tttcgaccga tcatgcttga aagtcccgat gaacggggaa ctttgccgcc
     1321 tcagtcggct ctaattagca ctccctttgt cagagtgcta atattgactc aaccagttgt
     1381 ttgttatcca gaaaaaggaa tgctttatcc gatgacgcaa cgctcgcaag cccttgccct
     1441 gccacagccc aatgcgctgg cactggcaat ctccaaccca ggcgccctgg gtaccattga
     1501 ggcctacatc ggcgccgtca accgcatccc catgctgtcg gccgagcgcg aggccgaact
     1561 cggtgaacgt gtgcgcgacc acatggatct ggacgcggcg cgtgaactga tcatgtccca
     1621 tttgcgcctg gtggtgtcgg tcgcgcgcca ttacctgggg tatggtctcg cgcatgccga
     1681 tctcatccag gaaggcaata tcggcctcat gaaggcggtc aagcgcttcg atccggcgcg
     1741 gggtgtgcgc ctggtgtcgt tcgccgtgca ctggatcaag gcggaaatcc acgaatacat
     1801 cgtgcgcaac tggcgcctgg tcaaggtggc caccaccaag gcccagcgca agctgttctt
     1861 caatctgcgc cgtatgcgcc cggatggtca aacgcttgat tccacccaag tcgacgatat
     1921 cgcgcgcgaa ctcaacgtgc ggccggaaga tgtcagcgag atggaggtcc gcttgtcggg
     1981 ccgcgaactg gccctggatg cacagcccga cgaagacgac accttcgccc ccatcgcgta
     2041 cctgtccgac gagggccggc aagaaccctc ccaggtgctc gagcgccgcg cgcacgacac
     2101 cctgcagggg tccggcctgg aacaggcgct cgaggcgctc gacccgcgct cgcgccgcat
     2161 cgttcaggcg cgctggctgc gcgatgaagg cggtgccacg cttcacgagc tggctgcgga
     2221 attcggtgtg tcggccgagc gcatccggca aatcgaggcc gccgcgctca agaaaatgcg
     2281 tggtgcgctg gccggatgaa cccgtgcgcg gcgtgattcg aagattcgat acatttagat
     2341 gcaaatacgt tgagggaatc gccgaactaa tcgagatgat cgcggactaa tctttcgtag
     2401 acactgcagg ccggccccgt tgtcggcaca ggcgctctat catccgcttc ggcttctcct
     2461 cttcccctcc cttttgaagc ggatgtttat gggacccttg caacaaggtg tcccattttt
     2521 tttgcccaaa aaatcagtat ccttgtaact gcgcctcgcg ggcgccggat cgcggccgtt
     2581 ttgcacccgc cgggtgccgc cccgatgggg tgtgccgggc gcaaccggcg ttcaatgtcg
     2641 attgggaagg cgatcatcta acaggggatt ccatgatgac ggttcgtggc gggtcgccgt
     2701 ccagaggtgg cgtatgctga ttgggcagtt ggtgttcgga ctggccggat tgctggcatt
     2761 ggtgtctttc atgccaccgc tcgccgggcg cctgcgtgtg ccgtattccg tgctgctcgc
     2821 cgttgtgggt ttcatcctgg gaatcatcat ccacgtgcat ggctgggcgc ccccggtcat
     2881 gggcgacttc cttgactccc tgcagcactt cgaagtgtcg tccgagacct tcctgttcgt
     2941 gttcctgcct gtcctgctgt tcgagaccgc gctggcgatg aacgtgcgcc ggttgatgga
     3001 tgatctgggc cccatcctga tgatggcgat cgtggccgtg ctggtgtcca cggtggcggt
     3061 gggctatgtg ctcagcgcgg catccagcta cggcctggtg gtctgtctca tgctgggcgc
     3121 catcgtcgcc accaccgacc cggttgccgt ggtgggcgtg tttcacgagg tcggcgcgcc
     3181 caaacgcctc aacacgctgg tcgaagggga agcgttgttc aacgacgcgg cgtcgatctc
     3241 cctgtattcc gtgttgctgg cgatgctgct gggcggcggc gcggtatcgg cgggctcggt
     3301 gctgtccaca ttcctgatca atttcctggg gggcggggtg gcgggcttcc tcatgggccg
     3361 tctggcctgc tggctggtga tgtggctacg cggctggccc acggccgaga tcaccctcac
     3421 ggtggccttc gcttatctca ccttcttcct gtccgagcat tatctgggtg tgtcgggcgt
     3481 tgtcgccacc gtgatcgccg gcctcgtggt ggggtcgacc ggccgcacac gcatgtctcc
     3541 ctccaccttc gaacaactgc gtggggcgtg gtccgaattc ggtttctggg ccaattccct
     3601 gatcttcgtt ttcgccgcca tgctgatccc gcgcatgatg gccgacgtca cctggcacga
     3661 ggtcgcgctg gtcattctgt tgttcttcgt ggcgctcatc gcacgcgcgg tcatggtctt
     3721 cggcgtgctg ccgctgctgg gccgcacccg tttcggcacc cgtgtcagcc tttcgtacca
     3781 ggtcgtgatc gcctggggcg ggctgcgggg ggcggtgtcg ctggctctgg cgctggccgt
     3841 cacggagcag gtgctcgtgc cctaccaggc gcgccagttc gtcgccgtgg ccaccaccag
     3901 cttcgtgctc atgacgctgt tcatcaatgg catgacgctg cggcccctga taaagcggtt
     3961 gcgcctggat cagctatcgc ccatcgagcg cacgatacgc aaccaggtgc aggtcatggc
     4021 gttgagcgat ctgcaggaca agaccgccga acttgccagc cgggagcaca tcggccccga
     4081 ggtcagtgcg cgcatccacg tcgtcttcga tgcaagcctg gcaagcgtgg ccgacgccca
     4141 ggtgcgtcag ctgtcccacg acgagcgcgt caccgtgggg ctgatgatga tggcggcgcg
     4201 ggaagaggaa atgttcttcg atgtgctcaa ggcccgcatc gtggactggc gcatggccga
     4261 atcgctgctg gcccgcgccg aacgcatgag cgatgcggtg cgcaatggcg ggctcgccgg
     4321 cttcgagtcc gccatcgcgg ccgacctgcg ctattctctc gggtttcgcg ccgcgctgcg
     4381 cacacactat gtgttcggct tccagggttg gctggcgcgg gaactggcca accgcttcac
     4441 gaatctgatg agcaagcgca gtgtggcgca gcggttgatc cagttcgccc aaacggagat
     4501 ccgcccgctg ctgggttcgg aagccaccga gaccatcatc ggcgcacatc agcgccgcct
     4561 tggtcaattg gaaaacgcct tgcaggcgct cagcctgcaa taccccttgt tcgcctcatg
     4621 gctgcaggaa tcctatctgg gccgcatggc gcgcgcgctc gagcggttgc gctatcgcga
     4681 catgctgtcc cagttcctga tttccaacga ggtgtatgcc gatctggtcg atcagatcga
     4741 gcaacgctgg cgctacgtgg atgagcgccc ggccctggac atcgcactga gcgccagcga
     4801 cctcatcaag cgcgtgccct tgttcgaagg cttgtcggcc gactcgctca aggccattga
     4861 aaagttgctc aagccgcgcc tgatgctgcc ggaccagcgc atagacatcc agctgggccg
     4921 cacacgcgcc atgtacttcg tggcctcggg cgccgtgtcg ctgcccttgc ccgacggaac
     4981 cacggccgag cttggttcag gcgaattctt cggcgagatc gcgctgttgt ccggcaagga
     5041 tttcgccacc gaggcgacgt cgttgggcta cagccgtctg ctgcgcctgc tcgcacgcga
     5101 cttcgacgct ttgctggcgc gtgacgcggt gttgcgggag aagatcgaga aagtcgccaa
     5161 gcagcggctc agggcgctgg aggtctggca ggaattccag tcgggcaagc gtcagtacga
     5221 gccgttgcct gtgtcgccgg agcgggaggc tgagaatggc ggtccgatgg gcgaggtcga
     5281 cgcccacgca aacgcgggcc gggttgccag cgcgccgggc ggcgaggcgg cgcagtctgg
     5341 cgaacacgtc gagccaggcg accgaggccc tacagcagca acctgatgtc gtgggccacg
     5401 acatcaggcg gcacggtgga attcagaatg acgcgcgtct cacccttctg atcgatgatg
     5461 tagaacatcg acgaatggtt catggcgtat tggccgggga ttttcgacga ttccttggcg
     5521 tagtacgtct tgaacgattt cgctgtcttc gccagttgtt cgggggtgcc gcccaggccg
     5581 acgaatgaag ggtcgaaggc tttggcgtaa tgatcgacga cggccggcgt gtcgcgcgcc
     5641 ggatcgaccg tgatcatgat gacctgcgtt ttcttcgcgt cgcctttcaa gagcgccatg
     5701 gtctgtgcga gctcggccat ggacgtcgga cagacgtcgg ggcagtgcgt atagccgaag
     5761 tagacgacca cgaccttgcc tttgtaatcc gcgagcgtgc gtacatggcc ggtggcatcg
     5821 gtcatggaca tgtcgcgccc caggtgcgtg ccggttacgt ccgatccggt gaacgcgggc
     5881 ttgtcgttgc cgcagcccgc caatgtcacg gatagcatgc ccgccgccag ggcggcggtc
     5941 acggcgcgcc gccgggcgga aaaggcgaaa gagttcatgt cagaacatca tccgcagcca
     6001 gtggtcgacg agcagcgcgc cgaacagcag agacagatac agaatggagt agcggaaaag
     6061 cttgcgggcg aggtcatcgc tgtattcgcg gtacaggcgc cacgcgtagt ggatgaacag
     6121 gccactgagg acgaccgccg ccagaagata ggcgaaacca ctcatgcgga tggcgaacgg
     6181 catgagcgac gccgcgaaca gaatcagact gtagagcagg atgtgcagcc gcgtgaactg
     6241 ttggccatgt gtgacgggca gcatgggcag gcccgatttg atgtagtcgt tgttgcgata
     6301 cagggccagc gaccagaagt gcgggggtgt ccagatgaag atgatgagca ccagcaacca
     6361 ggcttcggcg ggcacggaat tggaaatggc cgcccagccc agcgccggtg gcatggcgcc
     6421 ggaaagcccg ccgatgacga tgttctgcgg tgtgagtggc ttgagaatag ccgtgtagat
     6481 gacggcgtag ccaacgaagg tggcgaaggt gagccacatc gtcaggggat tgaccaagtt
     6541 gtacagcacc agcatgccga cgccgcccag cacacccgag aacgacaaca cttgcgcgtt
     6601 ggagatcgtg ccacgcgccg tggcgcgccg agccgtgcgc agcatgcgcg cgtcgacctc
     6661 gcgttcgatg aggcaattga tggagaacgc ggctgcggcc agcagccaga tgccgaccgt
     6721 tccggcgacc accgcgcgca ggtcgggcac acccgggctg gccaggaaca tgccaatgac
     6781 cgcgcagaaa accgcgagtt gagtcacgcg tggtttggtc aggacgaggt actggcgcag
     6841 caggccgggc gatggggccg aaagagaggt catgatggta ttttagcgcc gtttttggcg
     6901 cggctcagac gcaccagcaa ggtaaccgtg gccagcacca ggcccgccgc gccgccgttg
     6961 tgcaacaccg ccaccgccag cggccactga aagaatatgg tcgtcaggcc agtgcagagt
     7021 tgcgcgagca gcaaggccag catcagcgtg gcgggcccgc gcagccctgc gtcgcggcgc
     7081 aggcgaaaac ccatcacacc caggtaaatg aacacgatga aggcgaaatt gcggtgcgtc
     7141 cagtggatgg cggtgagcgc ggcctgggag atcatggcgc cggaaggcag catgcccagg
     7201 gcgcggatga gtgagtaccc gtcgcggaaa tccatggtgg gcagccattg gccgtggcag
     7261 gtggggaaat ccatgcaggc cagggcggca taattcgtgc tcacccaccc acccagggcg
     7321 atctgcgtga acagcagcgc cagccccact cccatccagg gtagggcgga ccccatcgcc
     7381 ggctggacgg gaagatggct tttctcgcgg gcggcgagcc aggtcatgag cgccagcagg
     7441 gacatgccca gcagaagatg cgtggtgacg atgacgggca tgagccggtg cgtgaccgtc
     7501 caggcgccga acgcgccctg cacgcagacc gccaccagcg ccacagtggc caggtatggg
     7561 gtgttgccca gttgccggcg gtagcgccac gccatgacca cgatggcgat gatgagcaac
     7621 cccagtatcg accccgcgta gcggtgcacc atttcaatcc aggccttgga gaagctcacc
     7681 gcgccgtatg gcatcgcctt cagggcgcct tcgatgtggc tggccgcccc gatgggggtg
     7741 acatgcccgt agcagcctgg ccagtcgggg catcccagcc ccgaatcggt caggcgtacg
     7801 aacgcgccga acatgatgag atccagtgtg aggaaccagg tcgtgaagac cagtttgcga
     7861 tagcgagcct tgatgcgatc caacatgtcg ttcagccgat ctgggagttg tgcaacagtt
     7921 tcttgaggtc atcgcgcacc gagatcggat cggcgttggc gggaaaccgc agcatgagat
     7981 gaccgagcgg atcgatgacc cacatggggt cgcgtagcgc cgcttcccgg gtcgttccgg
     8041 cacgcggcgc caggaacgcg gccagttgcg ccggtttggc gcgcacgatg tgcgtgccgc
     8101 cataggcctt cagcacatcg gcgggaacgg gcgcatcgtc ggtgacgaac cagacgcggg
     8161 tgagccgctc tacgttcttg ccctggctgg cgtgcgcatt gcgcaggatg aacagcttgc
     8221 gcacgcaggc cttggggcag gcgccctgat cggccgtcag caatatccat ttgcctttca
     8281 ggctggacag gtcgaacggc ttgccgtcca gcgtcgtcag cgccagcgcg ctcgcaggcg
     8341 gaatagggcg ttgcggctgg acgaatgttc cgtagttggt ggtgccgctg ggccgcaggc
     8401 ccagcgacgg gaagtaatac gccagccagg ccagcacgac cggtgcgatg cacacggcga
     8461 gtatggcgta gagcgggaac atggagcgct gtttgcgtgc cggcgtgccg ccgggggtgg
     8521 atgcttgctg agtcacggtg agtccttggg aggtgtcctt gtcggagggt ggcgagatca
     8581 cgccgagggc tggggggaag agtgtctggc acgccagcgg cgcagcgccc gccaggcgac
     8641 caccagccag gcgccggccg cgatggcggc gaagctgaac cattgcaggg catacccatt
     8701 gttacgcgcg tagtcgatgg acggcctggg ccagtcgcgc gccaggctgt cgccgcccgg
     8761 tgaggtctgc tccagcacgg ccggcacgaa tttgagcccg gtcgcgcgcg agtagtcgtg
     8821 aaggttcaga ttctgcaact gcggcaacgt cccaccgggg gcggggaatg tcgtgggcag
     8881 cttatccggc gaagcgccgg agaagcgcca gagctcgaac aggcgcggca cgcgggaaac
     8941 cagcgtgccc gccacgtcct gaggccccgg ggcggctggc gccacgtccg gtgtcttgcc
     9001 ggcccccacg aaaggcaacc agccgcgcag aaccagcaca gcggtgtggc tggcggcgtc
     9061 gatcaacagt ggcgtggcga cccaatatcc cggacgcccc ttgtcgttgc gatttagcag
     9121 tacagtgaac ttgtcgagcc acacgccgcg ggcggtggcc ggatgccact ctttcagggt
     9181 ggtcctgggc gaatccgcgg caatcgacag tggtgcgccg tgctcgccgg cttcgatggc
     9241 atgcatggtg ctcttgcgca gctcggcacg ccggagctgc caattcccca gtggaatgaa
     9301 aaccgctatc atgacgccca acaaaaacag ggcggcaagg gttcggatag gtgagtgcgg
     9361 acgtgccatg tttgaaattt tccagcttta ccggaggccg aaatgcatct catcgtcatt
     9421 gtggcgttcc tggggatcgt ggctagcctg ggctcggccc tgatcagtct gatgcgcgac
     9481 aagggtacct ccaaccgtac cgtcaacgcc ttgaccgtgc gtgtgggcct gtcgatcgcg
     9541 ctgttcctgt ttgtgttgtt cgcccatcac atgggctgga tccaggccac cgggttgacc
     9601 cagcactaag gaaaaaggcc ggcaaagccg gccttttttt cgcggcgttt cgcgctggtg
     9661 gggattacag ccagtagacg aacagataca gcccgagcca caccacgtcg acgaagtgcc
     9721 agtaccacga cgccccttcg aatccgaaat gatgattcgg ggtgaagtgg ccccggatca
     9781 ggcgcagggt gatgatggtc agcatggtgg cgcccaggat cacatggaac ccgtggaagc
     9841 cggtcagcat gtagaacagc gagccgaaag cgcccgaatc gaacttcagg ttcagttcgg
     9901 tgtaggagtc gtagtattcg tgggcctggc aggcgacgaa cgcgaacccc aggatgacgg
     9961 tcgcgatcat ccagaagatg gtcttggcgc ggtggttggc ccgcagcgcg tggtgcgcga
    10021 tggtgatcgt caggccggag gtcagcagca gggccgtgtt gatcgtggga agccagaacg
    10081 ggccgacggc cttgaacggt tcgatggtgc cggcggggcc gaagttgggc cagacatcgg
    10141 cgaaatgcgg ccacaacagg gtctggtgat cgaggttggt gagccacggc agcgagactt
    10201 cgcgcgtgta ccacagcgaa ccgaagaacg ccccgaagaa catcacctcg gagaaaatga
    10261 accacgacat gctccagcgg taggagtgat cgaccttgcc gctgttcatg ccgccttcgg
    10321 actcgccgat ggcgtccttg aaccagaaga acagcgtcgt cagcatacag atgacgccga
    10381 gcaggaacag ccacgggccg ggctcgaagc cgttgatcca gagtgccgcg ccgatcagca
    10441 ccatgagcat cgagatactg gcctggaccg gatggctcga atgggccggc acgtagtaat
    10501 aaggcgccgt ggttccttga gccgagtgac ttgcactcat gttgttctcc tgatacgaat
    10561 tttataaacc tacaaaacca ctttataaat ctaaaagacg actacgtcag attcgccacg
    10621 aacagcctgg ctatcgtgac cagcaccaac acgaagacca gcgtgagaag caggccgatg
    10681 atgatcaggt agcccgggct gatgcccttg gcgtcctttt cctggtcctg cctcctgcgt
    10741 acccccagga acccccagga caccgccttg agcgtttgca ggagactggc tctgtcttcc
    10801 tgcgggcgcg gattgtcgtc gtccatgggg ttcccggctg gcggctacgc cttgaagccg
    10861 ctccagaacg tccaggccat gatggcgatg acgaaaatca gcaggatgat tccggtgcgg
    10921 aggctacggc ggcgttgctc tggggtcata cgttaattga caatcggtgg ggtgacgaaa
    10981 gtgtggtgcg gggcgggcga tggcaccgtc cactccaggc cttcctcggc cccccagggg
    11041 ttggcggagg cgcgttcgcc acgacccgac acggccttcg ccaccacgta gatgaagatg
    11101 atctgcgaca ggccgaacca gaacgcaccg atggtcgcga tctggttgaa atcggtgaac
    11161 tgcggcgcgt aatcggccac acgacgcggc atgccggcca gacccaggaa gtgcatgggg
    11221 aagaacccca cgttgaacga gatcatgctc atccagaagt gccacttgcc gagcttttcg
    11281 tcgtacatgc ggcccgacca cttgggcagc cagtagtacg tcgcggagaa catcgcgaac
    11341 agcgcggagg ccaccagcac ataatggaag tggcccacga cgtagaaggt gtcggtgagc
    11401 tggatgtcga ccggggccag cgccagaacc aacccggtga agccgccgat catgaacacg
    11461 aagatgaagc cgatggcaaa cagcatgggg gtttcgaacg acagggagcc gcgccacatg
    11521 gtggccaccc agttgaagat cttcaccccc gtggggatgg agatgagcat ggtggcatac
    11581 atgaagaaca actgcgcggt caccggcatg ccggtggcga acatgtggtg cgcccagacc
    11641 aggaaggaca ggatggcgat ggcgcacacc gcatacacca tggaggtgta gccgaacagt
    11701 ttcttgcggg cgaacaccgg aatgacctca gagatcatgc cgaaggccgg caggatcatg
    11761 atgtacacct cggggtggcc gaagaaccag aacacgtgct ggtaaagcac cgggtcaccg
    11821 ccgccggccg cgttgaagaa cgtggtgccg aagtgacggt cggtgagcag catggtgacc
    11881 gaggcggcta gcaccggcat cacggccagc agcagatagg cggtcaccag ccagcaccag
    11941 cagaacatgg gcattttcat cagtgtcatg cccggtgccc gcaggttcag gatggtgacg
    12001 atgatgttga tggcgcccat gatggacgag gcgcccagga tgtggatggc gaagatggtg
    12061 aagtccatgc cggggcccat ttgcagggac agcggggcgt acatggtcca gcccgccgcc
    12121 tgcgcgccgc cgggcacgaa gaacgacacc gtcatcagcg tggcgccgac gggcagcagc
    12181 cagaagctga aattgttcat gcgcgcgaac gccatgtcgg acgccccgat ctgcatgggg
    12241 atcatccagt tggcgaagcc cacgaacgcc ggcatgatgg ccccgaagat catgatcagg
    12301 ccatgcatcg aggtgaactg gttgaagagc tcgggctgga agaactggat gccgggctcg
    12361 aagagttcgg ttcggatcag cagcgccagc acgccgcctt cgagcagcat gcagaacgag
    12421 aagatgagat acatcgtccc gatgtctttg tggttggtgg cgaacagcca gcgccgccag
    12481 ccgtatggca cggcatgggt gtggtcgtcg tgcccggtac cgggcggcac gtggtcgatg
    12541 gaaacgctgc tcatggtggc tccttcctac tcacccgagg gggctcgttg gccgccctgg
    12601 tatgaaatct gtgaatcttc gctggacatg ttcggttttg gtcgttgtct tagcgcaagg
    12661 ccttgacgtc ggaaggctgc acgacggggt cggggccttt gccggcgttg tcccaggcat
    12721 tgcgggtgta ggtgatgacc gcggcgacct ggacgtcgtc cagttggctg cgccaggccg
    12781 gcatggcggt gccggggtgg ccgttcagta cggtgaggat ctggcctttc atgggcgcca
    12841 gcacgaattt cttgtcacca tcgagcgccg ggaacgtacc cgggatgcct ttgccgttgg
    12901 cctggtgaca ggaaacgcag ttgctggtga agacctgctt gccgcgggcg accagttctt
    12961 ctttggagta tttcttgttg ggatcgtcgg acgacgcaag catggcgccc ttttccttct
    13021 tggcccactt gacgtaatcg gcgtcggaca cgaccttcac cacgatgggc atgtaggcgt
    13081 ggttcttgcc gcagagctcg gaacaggcgc cgcgataggt gccgatcttg gtggcacgga
    13141 accaggtgtc gcgcaggaag cccgggatgg cgtcctgttt gacgccgaag tcagggaccg
    13201 accacgagtg gatgacgtca tcggccgtga cgatgacgcg gactttcttg ttgaccggca
    13261 ccacgagcgg tttgtcgact tcaagcaggt agaagttgtc tttgggggct ttgccgtcga
    13321 tctgcgacat gggtgtcttc agattggaca ggaagtgcac gccggcggcg gcgccgtcca
    13381 ggtagtcgta gccccatttc cactgatagc cggtgacctt gatggagagg tcagggctgg
    13441 aagtgtcttt catggcgacc acggttttcg tcgcggggat ggccatgctg atgatgatga
    13501 ggaacggtat gaccgtccag gccacttcca gggccgtgtg ttcgtggaac ttggatgcca
    13561 catggccgcg cgacttgcgg tgcatgatga tggagtagaa catgaccccg accacgccaa
    13621 cgaaaatgat gacgcagatg atcatgatca tccagttcaa gtcccagatg cgctgggaaa
    13681 tggtggtgac tcctttatgc aggtcgagct ggttgacctg tggtccaccg ggcatgtttt
    13741 caatcttggc gctcgcggca gccatccata gccacgcgcc agtccccagt atccctctca
    13801 acttcttcat gctgacctct aaatacggtg cgtcatcttc gcgcggcaac cgtcgatcgg
    13861 ctggccggaa aacgcccgcc gcattcgaag agtacacagt gatatggatg gcaggctcgt
    13921 aactgctatg cctcgaaccg cctgctcgaa ttacttggag actgccagat tatagcgaat
    13981 gaaaatattc ttgcttaacc tttccttaca gccgggccgg ctcgccaatt cccctgttgt
    14041 atggtcgcaa taccccgatg gtagtacggt tgcgcggtac actgcgccaa gaccccaacg
    14101 cttgcactac aatttccgca tgacatcccc cggcgccggt tcattgcccg cggctataga
    14161 agtacgccgc gtggcgcttt tgcgttcgct ccagcagttg ccctccaatg gctctcgtcc
    14221 actgaccgta tcggccaggg tggccggctg ggtcacggcc agggcgaccg cttcgctgcg
    14281 cggtttgccc ggtgtgcgca tcgaggacga ggccgtgcac atcaccgcgt cgacgtcgcc
    14341 aaggttatcc ctcaacgccg tgttggcccg cgtggcccag gtgctggacg aggcgggctg
    14401 catccgtgcg tggcgcgacg aaatgctcga tgtggttggc gaggggcgcc gcctggcggc
    14461 gatcgagcgc ggtgcgatgc ggcccctggg gttgttgacc cgggccgtgc atctcaatgc
    14521 gtggacaccg caaggcgaat tatggattgc gcggcgcgcc ctgaccaaga gcaccgatcc
    14581 cggtaaatgg gatacgctgg tcggcggcct ggtgggcgcg ggcgaggcgc ttgataccgc
    14641 cttggtgcgc gaagcggccg aagaggccgg cctcacgcca gaggccatgg ccggtcattc
    14701 gccgttgcgc atcatcctgc gcatgcacaa gcgcctgccg gaaggctatc aggtggaaga
    14761 cttgttcatc agcgaatgcg tgctggacga tggcgtggcg ctggccaacc aggatgggga
    14821 agtcagcgaa ttccgccgcg ccggggtcga agaggtcttg tccctgatgc gggcacgcgc
    14881 tttcacggcg gaagccgaac tggtgctgct cgaagggata cgcggccggc ttggcgtggc
    14941 gacaaactga aatcgtggcg tgcgtcgcgc caccgggtca caagtaagcc cgcgtggccc
    15001 tcacgatgac ggtttggcca atccgttgtg ttcaacgggc gtgcgggctg tctggtggtg
    15061 gattcccttg cgcggatgcc ttgcccgact cgacgggctg gctggcgacc caaccgcgta
    15121 gcgcctcttg ggcgtcgcgt gccagattgt tccagctgac gtcatggcgc gcgttgaata
    15181 tcagcagggc gtagggctcg gccagcgccg cggcttcggc gcacaaagca ctttcctcac
    15241 cggtcgaagg cgaccgggca cgccaatagt tgatggcgga ttcgagctcg gcaagcgtgt
    15301 acgaaggctt catggcgggc gcggattaga cggtgcgggg gtgttcagtt taagctagtg
    15361 gcaccgcaca cgatagtgcc atgccgagga gtcatcatgc ccgccgaaag ccccaatccc
    15421 ttcgtacttc ccggcctggg ccaaaccggc gaccaggcgc agaacccgct gctggccggt
    15481 atggaaatga tgcgccaggc gtggcagggg ctggccagtg cgggcgggct gagccagtcg
    15541 gcgatggccg cgcccatgac gctcgaagag ctcgacaagc gcatcgccga tctgcgcgcg
    15601 gtggagaact ggctgcgcat gaatctgtcc atgctgacca gcgccatcca ggggctggaa
    15661 gtgcagcggg ccaccatctc caccttgcgc tccttcgtcg aatcggcggc cggggccggg
    15721 caggccgccg ccggccagtc gccgcttgaa gccgcgctgg gcatccacaa gcccgacgcg
    15781 ccggcgcagg ctggcaagaa ggccaaaggc gcggccgctg acggcgcgtc ctctggcgcg
    15841 tcggccatgc cgtcgtccgc gcaggcctgg tggaacatgc tgcaaggcca gttcgacacg
    15901 ctggcggccg ccaccgcggc cacactgcgg ggggccgatg acgccaagac agccgcgcag
    15961 cccggcgcgg gggcgggtgc ggaatctgcc gtaaaatcga ccccttccaa gacggccaaa
    16021 ggccgcaaac cgtcatcggg cacgcggggc cgggcggcca cgcccagaaa gacacccggt
    16081 ggggcgggcg aataagtggt cgacggcggg agcattcctg gccggtgcat tcaataagag
    16141 aacgaaccca tgttgaatat cgtcatcctg gccgcgggcc tgggcaagcg catgcagtcc
    16201 gatctgccca aggtcctgca tccggtggcc ggcaagtcca tgctggcgca cgtgctcgac
    16261 aacgcccgcg aactgaatcc cgaccagatt gtcatcgtgg tggggcatgg cgccgatcgc
    16321 gtccgccagg cattcacaga tgaaaaagac gtggcgttcg ccctgcaggt gccgcaacag
    16381 ggcacgggcc atgcggtggg cctggccgcg ccgctattgc gtgacgatgg ggcgtcgggc
    16441 acgacgctgg tgctgtatgg cgacgtgccc ctggtccagg ccgacaccat gaaacgcctg
    16501 atcgccgcgc gcgcccaggg catggccgtg ctcacgcaga ggctcgatga tccgagcggc
    16561 tacggccgca tcgtgcgcga ccaggcgggg cacatacggc gcatcgtcga acacaaagac
    16621 gcttcacctg ctgaacaggt catcaatgag gtcaacaccg gcatcctggc cgtgcccacc
    16681 gcgtggctca aggccgcgtt gccgcgcatc ggcaacgaca acgcgcaaca ggagtactac
    16741 ctgacggacc tcgtcgggtt ggcggtgggc gatggggtcg cggtcaattc ggccgagccg
    16801 ggcgctgatt gggaaaccct gggggtcaac agccgtatcc agcaggccca ggtcgaacgc
    16861 gtctggcagg gcgagcaagc ccggcgcctg ctcgagcaag gggtcacgct ggccgatccc
    16921 gcgcgcctgg acgtgcgcgg gtcgctggcc tgcgggcgcg acgtctatat agatgtgggc
    16981 tgcgtgttcg aaggtgacgt ttcgcttgcc gacggcgtgc gtatcggccc cttctgcgtg
    17041 ttgaagaacg cggaggtggg cgcgggtacg cagatcgacg cctatacgca tgtgcaagag
    17101 gccgtcatcg ccgacgacgc ccgcatcggc ccctatgccc ggctgcgccc cggtgccgac
    17161 atcggcgcgc aggcgcacgt gggcaacttc gtcgagatca agaaaagcac gctggggcgc
    17221 ggctccaagg ccaatcacct gacctacatc ggcgacgcca ccattggcca gcgcgtcaat
    17281 gtgggggccg gcaccatcac ctgcaattac gatggcgtca acaagttccg caccatcatc
    17341 gaagacgatg cgttcatcgg gtccgacacg caactggttg caccggtcac ggtggggcgt
    17401 ggcgccacgc ttggagccgg caccacgctt acccgtgacg cgccggccga tcaactgacc
    17461 ttgtcgcgtc cgacccagaa atcaataccg ggctggaagc ggccggtccg caagggttga
    17521 ggccgcgcgc ctcggcccgc ccgctttcat gcgaatcctg gaactgaact tcgagcgcgg
    17581 gtggcgcggg ggcgagcggc agaccctgta ctgcatgcgg cagttccgcc gggccggcca
    17641 cgatgtctcg ttgatggcgc ggcgcggtgg caagatcgcc gcggtcgcgc gggccgaagg
    17701 gttcaacgtg atcgaacagg ccggccccgc gggccaggcc gcgtacctga tggcgtcggg
    17761 caaccatttc gacatcattc atgcgcaaac cgccaacacc atcacctggg cagccctcac
    17821 gcgctggcgc caccgccggc ccgtggtgtt ttcgcgccgc accgacttcc atgtgcggca
    17881 tgatgcgcgc acccgtttca agtggcggca cgtggactgg ttcgtggcca tcagcgaatc
    17941 ggcggcggcg gagccccggc ggctggggct ggcgccgtcc atcattcgca gcgccatcga
    18001 acccgtgtcg gtcgacgacg cccgcgtggc gcgcttcgtg gaagaattcg gcctgcatgg
    18061 caaacgcctg atcggcacat ccgccgccct ggtcgacgac aaagacccat tgaccatgat
    18121 ccgggcggtg catcgcctgg cgggacagcg cgacgacttc gtcttcgtgc acctgggggg
    18181 cgggggcgac gccgaaccgg ccgctcgcgc cctggtcgat gaactggggt tgcaaaaagt
    18241 ctatctgttc gcgggctttc aatccgatgt cgaaagcctc tacagcatca tggacgtgtt
    18301 cgccatgagc tcagtcgaag aggcgctggg cagcagtgtg ctcgatgcct tcctacagcg
    18361 cgtgccggtc gtctccacgg acgcgggcgg gctcaaggaa agcctggccg acgggcgcgg
    18421 tgtgttgtgt cgggcacggg atcacgaggc gatggccgag ggcatgcggc gcatgctgga
    18481 cgacgcgggg ttccgcgacg cggccgtgga acgcgcctat gattatgtgc ggcgcgaaca
    18541 cgacgtggtc gagatgggtc gccgttatct ggaaggcttc gagcgcctgg gttcgaaaac
    18601 ggaacgtcgg cgcgggccat cgggaatcaa aaggtaagtc attgaagatc acggtattcg
    18661 gtagcggtta tgtggggttg gtcactggcg cgtgtctggc cgatggcggg catcacgtcc
    18721 tgtgcatgga cgtcgaccag gcgaaggtcg atggcctgcg ccgtggtgtc gtgcccatct
    18781 acgaaccggg tctggaaggc ctgattcaac gcaacatggc cgatggccgt ctgggtttca
    18841 catccgaccc ggccgaggcc gtggcgttcg gcgtgctgca gttcatcgcc gtgggcacgc
    18901 cgcccgatga ggacggttcc gccgacttgc agcacgtgct ggcggtggcg cgcaccatcg
    18961 gcatgcacat ggccgaccac aaggtggtga tcgacaaatc cacggtgccc gtgggcacgg
    19021 ccgacaaggt gcgggccacg gtggccgagg cgctggcggc acgcggcctg agcattgact
    19081 tcgacgtctg ctcgaacccg gaattcctga aggaaggcgc ggggatcgaa gacttcacgc
    19141 gtggcgcgcg catcgtggtg ggcaccgatt cggaacgggt caaggcgctg atgaccgaat
    19201 gctatgcgcc ctacaatcgc aaccacgaca aattcatgtt catggacgtg cgggccgccg
    19261 agctcaccaa gtacgccgcc aacgcgatgc tggccacgaa gatcagcttc atgaacgaga
    19321 tggcgaacct ggcggagcgc ctgggcgccg acattgaaca ggtgcgtcgc ggcatcggct
    19381 cggacccgcg cattggctac cacttcatct acccgggctg tggctacggc ggttcgtgct
    19441 tcaccaagga tgtgcaggcg ctcacgcaga gcgcggaaag caccggctat caggccgagt
    19501 tgctgcgcgt ggtcgaggcg gtgaaccggc gccagaaaca gactctactg cagaaactcg
    19561 atgcggcctt caacgacgag gtgcagggca agac
//
LOCUS       DASTNK010000020         6340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_197311, whole genome shotgun sequence.
ACCESSION   DASTNK010000020 DASTNK010000000
VERSION     DASTNK010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197311"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            375..740
                     /locus_tag="VFR20_01560"
     CDS             375..740
                     /locus_tag="VFR20_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229234.1"
                     /translation="MNDEVPPEAAKGPSLLTLTHVIYALFALGIVSVFFLGAGAVAAL
                     IVAYLKRADAAGTVYAPHFDWVIRTFWWGLLWMVISFVLTLIFIGWITGAIAIIWLIY
                     RIAKGWLALLSGYAPSSNY"
     gene            complement(834..1106)
                     /locus_tag="VFR20_01565"
     CDS             complement(834..1106)
                     /locus_tag="VFR20_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEU0229235.1"
                     /translation="MATAQKPARKPNPAFMKPLTPSATLAAVIGPEAVPRTEVTKKIW
                     DYIKLHNLQDPANRRNINADSKLRPLFGKDQVSMFELTKLVSAHLK"
     gene            complement(1261..1683)
                     /locus_tag="VFR20_01570"
     CDS             complement(1261..1683)
                     /locus_tag="VFR20_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEU0229236.1"
                     /translation="MSYDPQNVFAKILRGELPSIKIHEDDHTLTIMDVMPQADGHVLV
                     LTKEPVAEIFGLSEAGAAACMRTARKAAIAVKAALGAEGILVAQFNGRAAGQTVPHVH
                     VHVVPRQNGEPLRPHAAKPEDPAKLQALAEKIVAAWPA"
     gene            1905..3101
                     /locus_tag="VFR20_01575"
     CDS             1905..3101
                     /locus_tag="VFR20_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEU0229237.1"
                     /translation="MYAKNIIESIQSFHEELTAIRQDIHAHPELGFQETRTSDLVASA
                     LTSLGYQVHRNVGRTGLVGVLHGKKNTSGRGIGLRADMDALPILETGGHGYASTHKGV
                     MHACGHDGHTTVLLGAARYLAETRNFNGTVNLIFQPAEEGLGGAQAMLDDGLFERFPC
                     DSIFGLHNWPGLPPGVIGVCPGPMMAAADLFDIHIEGRGGHGAHAYQTNDPIIIAAQL
                     ITALQTIVSRNVPAPDSAVVTVAAVKAGNIEAKNVIPRDALLCGTVRTFSPVVQEQVI
                     ARMEQIIAGIASAFNAKIEFKYNKLFPATVNTPEHAALVADVATELFGTDNVVPNLTP
                     SMGSEDFSVMLKQRPGAYFRLGQGGAEKGCVLHSSIFNFNDAVIPTGSAMFCRLVERS
                     LPLDAA"
     gene            3104..4174
                     /gene="pyrC"
                     /locus_tag="VFR20_01580"
     CDS             3104..4174
                     /gene="pyrC"
                     /locus_tag="VFR20_01580"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU0229238.1"
                     /translation="MSANVTSSLTITRPDDWHLHLRDGPVLDAVIGDVARQFARAIVM
                     PNLKPPVVSTALALDYRQRILAAMARAGVDGKAFTPLMTLYLTDNTSADEIARAHDSG
                     LVHAVKLYPAGATTNSDAGVTDLLGRCSGALAALQEVGMPLLVHGEVTDKDIDVFDRE
                     AVFIERVMIPLRRRFPELKVVFEHITTRDGADYVREAQGPIAATITAHHLLYNRNALF
                     QGGLRPHWYCLPILKRETHRRALVEAATSDSGRFFLGTDSAPHLKGAKEQDCGCAGCY
                     TSLHAMELYASAFDSVGRLDRLEAFASLNGPAFYGLPVNTGSLTLVRSALDIPRELAV
                     GESAVVPLAAGESLPWSVAAQD"
     gene            complement(4233..6065)
                     /locus_tag="VFR20_01585"
     CDS             complement(4233..6065)
                     /locus_tag="VFR20_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0229239.1"
                     /translation="MPATTLITLTDVQLAYGHHPLLDHADLAIGPGERIGLIGRNGAG
                     KSSLLKMLDGRVVPDDGEIMRLGGLKTATVEQEPDLDDTSTIHDALCGGSSHHEDWQR
                     PARARMLADQLGLDANALIASLSGGLRKRVALARALASEPDLLLLDEPTNHLDFTGIS
                     WLENLLATARCGAVIVTHDRRFLDAVATRIVELDRGRLFSFPGDYSAWHARKIEWLVA
                     EQAQNQKFDKTLAQEEVWIRKGVEARRTRNEGRVRRLEQLRRDRIERRERQGNVKLSI
                     DEGKRSGRLVAELDDVTYAYDERPIVSHLSATIMRGDRIGIIGANGAGKTTLIKLILG
                     ELAPQSGRARLGTNLAVGYFDQMRSGLDENATLADTINPGSEWVEIGNQRRHVMSYLE
                     DFLFPAARARSPVSSLSGGERARLVLARLFARPTNLLVMDEPTNDLDIETLELLEELL
                     QDYTGTVLLVSHDRAFLDNVVTQTLAAEGDGHWGEYVGGYDDWLAQRASSVIEPAPSA
                     QRSTKRGLSTKPETPPSVSGRQIRVGKITAWEQRELDGLPDVIADLESQQAALTARLT
                     DANLYRDAPDEVGKINAQLRELETKLDALFARWESLEEKQASGS"
BASE COUNT         1115 a         1913 c         2017 g         1295 t
ORIGIN      
        1 cccatcccga acaggaccgt gaaacgcctt tgcgccgatg atagtggacg tacgtctgtg
       61 aaagtaggtc atcgtcaggc tccttatctg taaagcaacg ccccgcccgg ctcaccccgg
      121 cggggcgtcg ccgtttgggg ccccccacca cctcgccccg ctccatcccg gcgcttgcct
      181 gccctccaag tcaaccaggc ttgttcggtt cacgccactc gcggcggccc gtttgtggac
      241 ctgaccgccc ctctccctca agccacccag gcttgccacc gtgcttcaca gcgcgggttt
      301 accttacaat acgagcttgc cattcccacc ctcggatggg tgggctgatc tggttctgtc
      361 tcaagggaga tcgtatgaac gacgaagtgc cgcctgaagc agccaagggc ccctctctgc
      421 taaccctgac ccacgtcatc tacgcacttt tcgccttggg catagtaagc gttttcttct
      481 tgggggccgg tgctgtcgca gcgctcattg tggcctacct gaagcgggct gatgcggcgg
      541 gcaccgtgta cgccccgcat tttgattggg tcatccgcac gttctggtgg gggctgctct
      601 ggatggtgat cagctttgtg ctgacgttga ttttcattgg ctggataacg ggtgcgatcg
      661 cgatcatctg gttgatatac cgtatcgcga aagggtggct tgccttgctg tcgggttacg
      721 ccccgtcttc caactactga atttcaagcc tccagccata atgaagaacg ccgccctgct
      781 gggcggcgtt tcatatttgg gcgcgcgttc gagacgcggg cgactggcgc ggcctacttg
      841 aggtgcgcac taacgagctt ggtgagctcg aacatggaga cctggtcttt gccgaacaac
      901 gggcgaagct tggaatcggc gttgatgttg cgacgattgg cgggatcctg gaggttgtgc
      961 agtttgatgt aatcccagat tttcttggtg acttcggtgc gggggacggc ttcggggccg
     1021 atgacggcgg ccagtgtcgc actgggtgtc aggggcttca taaatgccgg attgggtttg
     1081 cgggcaggtt tctgggctgt tgccatagag aaaagctcct agctatgaat gaaagatacg
     1141 acgctccgag cataccttga gatgtcgtga aacacaaagc gttttacgca ttcgcgtttg
     1201 tggaacgccc gggctgttgc caagacgtca ggggaaaccg cccgattgtt accactgctt
     1261 tcaggcgggc caggccgcca cgatcttttc tgccaacgcc tggagtttgg cggggtcttc
     1321 cggtttggcg gcgtgcgggc gcaacggttc gccattctgg cgaggcacca catgcacatg
     1381 cacatgggga acggtttgcc cggcggcccg cccattgaat tgcgcaacca atattccttc
     1441 cgcgcccaat gccgccttca ccgcgatagc ggccttgcgg gccgtgcgca tgcaggcggc
     1501 ggcgccggct tcagacagtc cgaagatttc ggcaacgggt tctttggtca gcaccagcac
     1561 atgcccatcg gcctgaggca tgacgtccat gatggtgagc gtgtggtcgt cttcatggat
     1621 tttgatgctt ggcaactcgc cacgcaggat cttggcaaaa acgttctgag ggtcgtagct
     1681 catgatggtt cggtaaggtg aagggtgaat gagttgaact aaggttccac gtttgtttca
     1741 gaggtggttt ccggtaatgc taccgcaact ggataacagt gtacgaggcg caggacgtaa
     1801 aggcgcggcg atggtgtgga aacggacgcg gccgttccgt tacgctatga tatgcacagc
     1861 ctgcggcggg cgccgttggc catcatttcc aagggtgccg gggcatgtac gcaaagaaca
     1921 tcatcgagtc gatccagtca tttcatgaag agctcaccgc cattcggcag gacatccacg
     1981 cgcatcccga actggggttc caggaaaccc gcacatccga tttggtcgcc agcgcgctga
     2041 cgtcgctggg ctatcaggtc caccgcaatg tggggcgcac cggcctggtt ggggtgctgc
     2101 acggcaaaaa aaacaccagc ggacgcggta tagggttgcg ggccgacatg gacgcgttac
     2161 ccatccttga gaccggaggg cacggctacg catcgacgca caaaggggtc atgcatgcct
     2221 gcggccacga cggacacaca acggtgctac taggggcggc ccgctatctg gcggaaacgc
     2281 gcaatttcaa cggcacggtc aacctcatct tccagccggc ggaagaaggg ctcggtggcg
     2341 cccaggccat gctggacgat ggcttgttcg aacgtttccc ttgcgattcc atctttggtc
     2401 tgcacaactg gcccggcctg ccgcctggcg tgataggagt gtgccccggt ccgatgatgg
     2461 cggcggccga tctgttcgat attcatatag aagggcgtgg tgggcatggc gcccatgcgt
     2521 accagacgaa cgacccgatc atcatcgcgg cgcaattgat cactgctctg cagaccatcg
     2581 tgtcacgcaa tgtaccggcg cccgattccg cggtcgtcac cgtggcggcc gtcaaggcgg
     2641 ggaatatcga agccaagaat gtcatcccca gggatgcgtt gctgtgcggt accgtgcgca
     2701 cgttcagccc cgtcgtccag gaacaggtga tcgcgcgcat ggagcagatc atcgctggca
     2761 tcgcgtcggc cttcaacgcc aagatcgagt tcaagtacaa caagctgttc cccgccacgg
     2821 tgaatacgcc ggaacacgcc gcgttggtcg ccgacgtggc caccgaactt ttcggcacgg
     2881 acaacgtggt gcccaatctc accccgtcca tgggatcgga ggatttttcc gtcatgttga
     2941 agcagcgtcc gggcgcgtat ttccgcctgg ggcagggggg agcggaaaag ggctgcgtcc
     3001 tgcacagttc catcttcaat ttcaacgatg cggtcatccc gaccggcagc gccatgttct
     3061 gccgcctggt ggagcgcagc cttcctttgg atgcggcctg accatgtccg cgaacgtaac
     3121 ctcctcgctg accatcacgc gccctgacga ctggcacctg catttgcgtg acggaccggt
     3181 gctggacgcc gtgatcggtg acgtggcacg ccagttcgcc cgcgccatcg tcatgcccaa
     3241 cctgaagccg ccggtggtgt cgaccgcgct ggcgctggac tatcgtcagc gcattcttgc
     3301 cgccatggcg cgcgccggcg tcgacggcaa ggccttcacc ccgctcatga cgctatacct
     3361 gaccgacaac acctcggcgg acgaaatcgc gcgcgcgcac gattcggggc tggtgcatgc
     3421 cgtcaagcta tacccggccg gcgcaaccac caattcggat gccggtgtca ccgatctgct
     3481 ggggcgttgc tcgggtgcgc tcgcggccct gcaggaagtc ggcatgccgc tgttggtgca
     3541 tggcgaggtc acggacaagg acatcgatgt cttcgatcgg gaagccgtgt tcatcgagcg
     3601 cgtcatgatt ccgctgcgtc gccgctttcc cgaactgaaa gtcgtgttcg agcacatcac
     3661 cacgcgcgac ggcgccgatt acgtgcggga ggcgcaaggt ccgatcgcgg ccacgatcac
     3721 cgcgcatcat ctgctgtaca accggaatgc gttgtttcag ggagggctgc ggccgcactg
     3781 gtattgcttg ccgatcctca agcgcgagac gcatcgacgc gcgctggtcg aagcggccac
     3841 cagcgattcg ggccgcttct tcctggggac cgacagcgct ccgcacctga aaggggcgaa
     3901 agagcaggat tgcggttgcg ccggatgcta cacatctctt catgccatgg aactctatgc
     3961 cagcgcgttc gattcggtcg gccggctcga ccggctcgag gctttcgcca gcctcaatgg
     4021 gccggctttc tatggcctgc cggtgaatac ggggtcgctc acactggtgc gctcggccct
     4081 cgacatccca cgtgagctgg ctgtcggtga aagtgccgtc gtgccgttgg ccgcgggcga
     4141 gtctctgcct tggtccgtgg cagcgcaaga ttgacggcgc ctggccagga gacctccctc
     4201 cgcgcttttt ttgccacggt ggcgcgaggg tgtcaggagc cgctggcctg tttctcttcc
     4261 aggctttccc agcgggcgaa caaggcgtcc agctttgtct cgagttcacg caattgagcg
     4321 ttgatcttgc cgacttcatc cggcgcgtcc cggtacaggt tggcgtcggt aagcctggcg
     4381 gtgagggcgg cttgctgtga ttcgaggtcg gcgatgacat cgggcaggcc gtcgagttcg
     4441 cgttgctccc aggcggtgat cttcccgact ctgatctgtc gtccgctgac ggacggtggc
     4501 gtttcgggct tcgtcgacag gccccgcttt gtggagcgtt gggccgacgg cgcgggctct
     4561 atgacagaag aggcccgctg agccagccaa tcgtcgtacc cacccacgta ttccccccaa
     4621 tgaccgtcgc cctcggccgc cagggtctgg gtaacgacat tgtccagaaa cgcgcggtca
     4681 tggctgacca gcagaacagt gccggtgtag tcctgcaaca gctcttccag caactcaagc
     4741 gtttcgatgt cgaggtcgtt ggtgggttcg tccatgacca gcaggttcgt ggggcgggcg
     4801 aacaggcggg ccagcaccag tcgcgcgcgt tcgccgccgg acaggctgct gaccggtgac
     4861 cgggcgcggg ccgctggaaa caggaagtcc tcgaggtagc tcatcacgtg cctgcgctgg
     4921 ttgccgattt ccacccattc gctgccgggg ttgatcgtgt cggccagtgt ggcgttctcg
     4981 tccaggccgg atcgcatctg atcgaaatag ccaaccgcca gattcgtgcc gaggcgggcc
     5041 cgccctgatt ggggggccag ttcacccagg atgagtttaa tcagggtggt cttgcccgcg
     5101 ccgttggccc cgatgatgcc gatgcggtca cctcgcatga tggtggcgct gagatggctc
     5161 acgataggcc gctcgtcgta ggcatacgtg acgtcgtcga gttcggcgac gagacgtccc
     5221 gatcgtttgc cttcatcgat ggacagtttg acgttgccct gccgttctcg acgctcgatg
     5281 cggtcacgcc gcaattgttc gagtcgccgc acgcggcctt cattgcgtgt gcggcgagcc
     5341 tcgaccccct tgcgtatcca gacctcttcc tgcgcaaggg tcttgtcgaa cttctgattc
     5401 tgtgcttgtt cggccacgag ccattcgatc ttccgggcgt gccaggctga atagtcaccg
     5461 gggaaactga acagccgccc gcgatcgagc tcgacgatgc gtgtggcgac cgcatcgaga
     5521 aaacgacggt cgtgcgtgac gatgaccgcg ccgcaccggg cggttgccag cagattctcc
     5581 aaccaggaga ttccagtgaa gtcgagatgg ttggtgggtt cgtccaacag caacagatca
     5641 ggctcgctgg ccagggcccg cgccagcgcg acacgtttgc gaagacctcc ggagagagag
     5701 gcgatcagcg cattcgcgtc cagccccagc tgatccgcca gcatccttgc ccgcgcgggc
     5761 cgttgccaat cttcgtgatg gctggaacct ccacacagcg catcatggat ggtcgacgtg
     5821 tcgtcgaggt cgggttcttg ctcgaccgtc gcggttttca gcccacccag tcgcatgatt
     5881 tctccgtcgt cggggacgac acgaccgtca agcatcttga gcagggatga cttgccggcg
     5941 ccgttgcggc caatgaggcc gatacgctcg ccggggccta ttgcgagatc ggcgtggtcc
     6001 agcagtggat gatggccata ggccagctgc acgtcggtga gggtgataag agttgtcgcg
     6061 ggcatggaga aacgaaaatc ggaagcggaa attgatacgg gagctattgt cgcaggtaca
     6121 cgcccccgta tgccagccgc tccgcgattc gatacaatgc tagcttgagg cgggccgggc
     6181 aatcgcggtg catgcgtgta tcgaggaagg tccggactcc acagagcgcg atagcggcta
     6241 acggccgtcc ggcgttgtgg cgcattgcgt cgccaccaag ccgaggaaca gggccacaga
     6301 gacgagtctg cggcgcgggc gtgcgacagc acgcacaggc
//
LOCUS       DASTNK010000021         5182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_938630, whole genome shotgun sequence.
ACCESSION   DASTNK010000021 DASTNK010000000
VERSION     DASTNK010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938630"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..521)
                     /locus_tag="VFR20_01590"
     CDS             complement(12..521)
                     /locus_tag="VFR20_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEU0229240.1"
                     /translation="MAFSIYTASVPVFRQYLGSLDAILHKAEKHAVDGKFDPDVLLQA
                     RLYPDMFPFVRQVQLAASFARDITARLGGTDIPKYDNTEHSFADLHALIVRTLAFIDS
                     VPQTTIEGHEDRKTATRVGTPQEKTYTAQSYLLTYGMPQFFFHITTAYAILRHNGVVV
                     GKRDFLGKF"
     gene            826..1296
                     /locus_tag="VFR20_01595"
     CDS             826..1296
                     /locus_tag="VFR20_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0229241.1"
                     /translation="MYFRNTDSKKSPTPLALALAGMLLAGAGFTSSAWAELPPLQHQG
                     AVTYVSGGIGIDESTSFKQAMSHYPLAMTFAEQEGKQGDYLADVKVVIKNARLNTVLD
                     TTAQGPYMLVKLQPGHYRVSATFKNQTKTRNITIGRKGSEHLVFEWKPVASANT"
     gene            1419..2360
                     /locus_tag="VFR20_01600"
     CDS             1419..2360
                     /locus_tag="VFR20_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0229242.1"
                     /translation="MTDTDTIIKTVIGKLRIKQLQLLVALSEQKSLRNAAKAMFMTQS
                     AASKALLEIEGMLGMTLFERTKTGLAPNPMGHCAIRYAKLITSELNNFCREVQDIQSG
                     AGGSLVIGTIMGAVPGVLVSSLNWLRESHPNLSVEIIEGTSRHLLSLLDDGRIDLMIG
                     RSSVSAEPYEYSYYPVSGEPISVVVGRGHPDTAEHMSFADLRGHRWVVYPKKMPLRAL
                     LEKEMDLAGLSLPANFIETDSTFITVALLRESVGMAALLPTDVANLFTEAGLIRILPI
                     ELKIPADTFGIVTRKGGATTAVAQRLIRFACDQTRTH"
     gene            complement(2373..3032)
                     /locus_tag="VFR20_01605"
     CDS             complement(2373..3032)
                     /locus_tag="VFR20_01605"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HEU0229243.1"
                     /translation="MKQETLRRHFHEALESAGLVAILRGIEPDEVVDVGGVLYEAGFR
                     IIEVPLNSPQPLRSIERLAASLPADTLVGAGTVLAARQVGEVLSAGGRLVVMPHSDNG
                     IIRAAIDAGMAVLPGVATPTEAFAALGAGAIALKAFPAELLTPTVLKAWRAVLPADVP
                     LVPVGGITPDNMQAYLDVGAAGFGLGSALYRAGDTAAAVRPRAQAFMARWRQATARST
                     G"
     gene            complement(3029..4069)
                     /locus_tag="VFR20_01610"
     CDS             complement(3029..4069)
                     /locus_tag="VFR20_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEU0229244.1"
                     /translation="MVEQRFIGLDWGTSSLRAYLFAADGRVLARREAPWGIMTLPPLA
                     EDGAPARSGGDETNRERRFSHAFWQLLGDWLGHPGTSRRVVACGMIGSSQGWREASYM
                     PVPVTVRDLAGSLTTIEVGQGVTLAIAPGIIEHGALPNVLRGEETQIAGVPALLAEAG
                     TDMSGRWLLGLPGTHSKWVAVRDGEIGHFDTFMTGEVYALLVQHSILGRSMGSAVLPE
                     DSRDARDTFLLGLGNACSESGKLGVLATMFSSRVLGLVSRLTGAQQREYLSGLLIGHE
                     IAGLLARVQVEWRQAHIALIGRAALCARYAEALRYFGREALILSEDAGVHGLWSLISS
                     DCGCDALQGCEK"
     gene            complement(4097..5029)
                     /locus_tag="VFR20_01615"
     CDS             complement(4097..5029)
                     /locus_tag="VFR20_01615"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0229245.1"
                     /translation="MSKRFEKARYKGVYPVVPTIFKDDGALDLEGQLRCVDFMIDAGA
                     DGLSLLANFSEQFLLADEERRVLTEAMLKHVAGRVPVIVTTSHYSTDICIQRNRHASD
                     FGAAMVMVLPPYHGATLRCAEAGVHDYFRRLSDASDIPIMVQDAPISGTLLSAAALAR
                     MAREIEHVSYFKIESAGAASKIRALIELAGDAVEGPWDGEEAITLLPDLDAGATGAMT
                     GGGYPDGIKAIFNAYMHGDREKAVSLYQQWLLLINYENRQTGIQTSKILMKEGGIIGS
                     DHVRSPLPAATTEAKAGLIETARRLDPLVLRWAA"
BASE COUNT         1042 a         1726 c         1518 g          896 t
ORIGIN      
        1 gattcagccc tctagaactt ccccagaaaa tcccgcttgc cgaccactac gccgttgtgg
       61 cgcaggatgg cgtaagccgt cgtgatgtga aagaagaact gtggcatccc gtaggtcaac
      121 aggtaagact gagcggtata ggtcttctcc tggggcgtac ccacccgcgt ggcggtcttg
      181 cgatcctcgt gaccttcgat ggtggtttgc ggcacgctgt cgatgaaggc aagcgtccgg
      241 acgatcaacg catgcagatc ggcgaagctg tgctcggtgt tgtcgtactt gggaatatcg
      301 gttccgccaa gccgggcggt gatgtcgcgg gcgaagctcg cggcaagctg cacctggcgg
      361 acgaacggga acatgtcggg atacaggcgc gcctgcaaca agacgtcggg atcgaacttg
      421 ccatcgaccg cgtgtttctc cgccttgtgc agaatggcat ccaggctgcc caggtactgc
      481 ctgaagactg gcaccgatgc cgtgtaaatt gaaaaagcca tgatgatcct cttgatgaaa
      541 gaacgaaaaa cagcctgttc attatagaaa ctccagactc gccgatatga cgtacagtct
      601 gttcaatacg gaactatcaa gcgtggaatc tgtcatattc tcgcatctcg aacggagctt
      661 cccgccactt tctggccatc gccatgcgtc gtctcaacgc accgcatacc cccgcacacc
      721 cggcgatcca caagaaccgg atgggcaccc tttcaggtgg cccgcctcgc gaaccattcg
      781 gcgcgaaaca gattccccat acaatggaaa gacaaggagt gcatcatgta tttcagaaac
      841 actgattcca aaaaatcccc caccccgctg gcgctcgcgc tcgcgggcat gctgctggcg
      901 ggtgccggct tcacgtcgtc ggcgtgggcc gaactgccgc ccctgcaaca ccagggcgcg
      961 gtcacgtacg tgagcggcgg catcggcatc gacgaatcca cgtcgttcaa gcaggccatg
     1021 tcacactatc cattggcgat gacgttcgcc gaacaggagg gtaaacaggg cgactacctg
     1081 gccgatgtga aggtggtcat caaaaacgcg cggctcaaca cggttctgga caccacggcg
     1141 caggggccgt acatgctggt caagctgcag ccgggccact atcgggtctc ggccacattc
     1201 aagaaccaga ccaagacccg caacatcacg atagggagaa aaggctccga gcatctggtg
     1261 tttgagtgga aaccggttgc gagcgcgaat acttgaccgc gcaatccgtc atgccccggc
     1321 gcttgcccac cgcaggcgcc ggtcaacgcg cgaaacagtg gctcgcgcag ccaggcattc
     1381 acagatcgcc aggcatgcga cgacatgcat catgagtcat gactgacacc gacacgataa
     1441 taaaaaccgt catcggcaag ctgcgaatca agcaattgca gttgctggtc gccctgagcg
     1501 agcaaaaatc gctgcgcaac gccgcgaaag ccatgttcat gacgcagtcg gccgccagca
     1561 aagcccttct ggaaatcgag ggcatgctgg gcatgacgct tttcgagcgc acgaaaaccg
     1621 gcctggctcc caaccccatg ggccattgcg ccattcgtta cgcgaaactc atcacgtcgg
     1681 agctcaacaa tttctgccgc gaggttcagg acatccaatc gggggccggt ggcagcctgg
     1741 tcatcggcac catcatgggg gcggtgccgg gggttctggt gtcatccttg aactggctgc
     1801 gcgaatccca ccccaacctt tccgtggaaa tcatcgaagg caccagccgg cacttgctga
     1861 gcctgctgga cgatggccgc atcgacctga tgatcggccg gtccagcgtc agcgcggaac
     1921 cctacgaata cagctactac cccgtcagtg gcgaacccat atcggtcgtc gtcggccgag
     1981 ggcaccccga caccgcggaa cacatgtcat tcgcggatct caggggccac cgctgggtgg
     2041 tctatccaaa gaaaatgccc ttgcgggccc tgctggaaaa ggagatggat ctggcggggt
     2101 tgtccctgcc cgcgaatttc atcgagacgg actccacctt catcaccgtc gccctcctgc
     2161 gggaaagcgt cggcatggcc gcgctgttgc ccaccgacgt ggcgaacctg ttcacggagg
     2221 ccggactgat ccggatcctg ccgatcgaac tgaagattcc ggccgacacc ttcggcatcg
     2281 tcacacgcaa gggtggcgcc accacagcgg tcgcgcagag gctcattcgt ttcgcctgcg
     2341 accagacacg cacacactga ggcgtgggca ggtcaccccg ttgaccgcgc ggtcgcctga
     2401 cgccagcgcg ccatgaatgc ctgcgcgcgc ggtcgaacgg cggcggccgt gtcgccggcg
     2461 cggtagagcg cgctgcccag gccaaacccc gccgccccga cgtccaggta agcctgcatg
     2521 ttgtcagggg tgatgcctcc gacgggcacg agcgggacat ccgccggcaa caccgcgcgc
     2581 caggccttga gcaccgtcgg cgtcaacagc tcagccggga acgccttcag cgcgatggcg
     2641 cccgccccca gcgcggcaaa agcctccgtg ggcgtggcca caccgggcaa caccgccatc
     2701 ccggcatcga tggccgcccg gatgatgccg ttgtccgagt gcggcatgac gaccaggcgt
     2761 ccacctgcgg aaagcacttc cccaacctgg cgcgcagcca ggacagtgcc cgcccccacg
     2821 agcgtgtcgg cgggcaggct cgccgccaac cgttcgatgc tgcgaagagg ctgcggcgaa
     2881 ttcaacggca cttcgatgat gcgaaagccc gcctcataca acaccccgcc gacatccacc
     2941 acttcgtcag gctctatgcc ccgcagaatc gcgacgagcc cggccgattc cagcgcctca
     3001 tgaaaatgcc gccgcaacgt ttcctgtttc atttttcgca cccttgaagc gcatcgcagc
     3061 cacagtcgct cgaaatcaac gaccacaacc cgtgaacgcc ggcgtcctcc gacaggatca
     3121 gcgcctcccg tccgaaatac cggagcgcct cggcatagcg cgcgcaaagc gcggcccgcc
     3181 cgatcaaggc gatatgcgcc tgccgccact ccacctgcac cctggccagc aaaccggcga
     3241 tctcgtggcc gatcagcagc cccgacaggt actcgcgctg ctgcgcgccg gtcagccgac
     3301 tcaccaggcc aagaacgcga ctactgaaca tggttgccag caccccaagc tttccggact
     3361 cggagcaagc gttgcccagc cccagcagaa aggtgtcccg ggcgtcccgg ctatcttcgg
     3421 gcagcacggc gctgcccatc gaccggccca ggatgctgtg ttggaccagc agggcataga
     3481 cttcgcccgt catgaacgtg tcgaaatgac cgatttcacc gtcccgcacc gccacccact
     3541 tgctgtgggt tcccggcagc cccagcaacc agcgaccgct catgtccgtg cccgcctccg
     3601 ccagcagggc cggcacaccg gcgatttgag tctcttcgcc acgcaagacg ttgggcagcg
     3661 ccccgtgttc gataatgccg ggcgcgatgg caagtgtcac cccctggccg acctcgatgg
     3721 tggtcaggga tcccgccaga tcgcgcaccg tgacgggcac cggcatgtag gaggcttccc
     3781 gccacccctg ggaactgccg atcatgccgc aggctacgac gcgacggctg gtgccgggat
     3841 gcccaagcca gtctcccagg agctgccaga aggcgtggct aaaccgccgc tcgcggttcg
     3901 tttcatcgcc accagaccgg gcaggcgcgc catcttccgc caatgggggc agagtcatga
     3961 tcccccatgg cgcctcgcgc cgcgccagca cacgcccatc cgccgcgaac aggtatgccc
     4021 gcagcgaact cgttccccaa tccagtccga tgaaacgttg ctcgaccatg tttgcctggt
     4081 atcgtgcccg cgaacgtcac gcggcccagc gcagaaccag ggggtccagc ctgcgagcgg
     4141 tttctatcaa tccggccttg gcctctgtcg tcgcggcggg cagcggcgag cgcacatgat
     4201 ccgagccaat gatcccgccc tctttcatca ggatcttgct ggtctggatg ccggtctggc
     4261 ggttctcgta gttgatcagg agcagccatt gctggtacag ggacacggct ttttcgcgat
     4321 ctccgtgcat gtacgcattg aagatggcct tgatgccatc cggataccct cccccggtca
     4381 tggcgcccgt ggcgcccgcg tcgagatcag gcagcagggt gatggcctct tccccatccc
     4441 atgggccttc gacggcgtcc cccgccagct cgatcagcgc tcgtatcttg gaggccgccc
     4501 cggcgctttc gatcttgaag taagagacgt gctcgatttc ccttgccatg cgggccaacg
     4561 ccgcggcgga caacagcgtg ccgctgatcg gggcgtcctg gaccatgatg ggaatgtcgc
     4621 tggcgtcgga aaggcggcgg aaatagtcgt gaacgcccgc ctcggcacag cggagggtcg
     4681 cgccgtgata aggcggcagg accatcacca tcgcggcgcc aaaatcgctg gcgtgccgat
     4741 tacgctggat gcagatatcc gtactgtagt gcgaggtggt gacgatgacc ggcacgcgac
     4801 cggcgacatg cttcagcatg gcctcggtca acacccggcg ctcttcgtcc gccaacaaga
     4861 actgctctga aaaattggcc agcaggctca ggccatcggc gccggcgtca atcatgaaat
     4921 ccacgcaccg gagctggccc tccaggtcga gcgcgccgtc atccttgaag atagtcggca
     4981 caacggggta cacgcccttg tagcgggctt tttcaaaacg tttggacatg atgttcacta
     5041 tacattctcg acttttaaaa caatcagcag atcacactgg gttgccaggg cacgaactcg
     5101 ttttgtccat agccatagat ctcgctcttt gagcgctcgc cggaggcgca gcggcgtatc
     5161 atctcgtaga attcccgccc ca
//
LOCUS       DASTNK010000022         6000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1124832, whole genome shotgun sequence.
ACCESSION   DASTNK010000022 DASTNK010000000
VERSION     DASTNK010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124832"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /gene="boxB"
                     /locus_tag="VFR20_01620"
     CDS             complement(<1..1151)
                     /gene="boxB"
                     /locus_tag="VFR20_01620"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEU0229246.1"
                     /translation="MSGINYSEKIPNNVNLSEDRALQRALEHWQPNYLQWWNEMGPEG
                     AQGHDVYLRTATSVQPDGWAHFDYTRMPDYRWGIFLTPQDGNRKIHFGEHLGRDVWQE
                     APGEHRANLRRIIVTQGDTEPASVEQQRHLGLTAPSLYDLRNLFQVNVEEGRHLWAMV
                     YLLHRYFGRDGREEADALLQRNSGDQDSPRILGAFNEKTPDWLAFYMFTYFTDRDGKF
                     QLCALAESGFDPLARTTRFMLTEEAHHMFVGESGVSRVLQRTCDVMNEIKTDDPADVR
                     AAGAIDLPTIQRYLNFHYSVTIDLFGADQSSNAAIFYGAGLKGRYEEGKRTDDHQLKN
                     DTYRVLDVANGGLREIEVPMLNALNEVLRDDFIRDSVAGIGRWNKVMEKR"
     gene            complement(1181..2839)
                     /gene="boxC"
                     /locus_tag="VFR20_01625"
     CDS             complement(1181..2839)
                     /gene="boxC"
                     /locus_tag="VFR20_01625"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HEU0229247.1"
                     /translation="MSQPMPLVDFRTHPGLYRHWVLSFDGVVATLAMNVDEDGGIRPG
                     YKLKLNSYDLGVDIELYDALQRIRFEHPEVRTVVVTSLKDRVFCSGANIFMLGLSSHA
                     WKVNFCKFTNETRNGIEDSSRHSGLKFVAALNGACAGGGYELALACDEILLIDDRSSA
                     VALPEVPLLGVLPGTGGLTRVTDKRKVRHDRADIFCTLVEGVRGQRAKDWRLVDELIK
                     PAQFTEGVNARALELADRSDRPANEQGVALTPLERSAGVDGLSYHCVDVCIDRAERLA
                     TWTVRAPDADTPDDIDGILAQGASWWPLRMARELDDAILHMRTNELDIGTWVFKTEGD
                     AARVLACDATLKRFGHHWFVRETVGMLRRTLARLDVTSRSLYALIEPESCFVGTLLEL
                     ALACDRSYMLDDPDADAPARIAVGESNFGDYPMVNGQSRLAHRFYDEAPALDAVRATL
                     GRFLSAREAEEIGLVTLSPDSLDWDDEIRLALEERRALSPDALTGLEANLRFGGQETM
                     ETRVFGRLTAWQNWIFNRPNAVGEKGALKLYGKGEQPSFDWNRV"
     gene            complement(3331..4260)
                     /locus_tag="VFR20_01630"
     CDS             complement(3331..4260)
                     /locus_tag="VFR20_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0229248.1"
                     /translation="MKNKAFELPPHSEPDNVDPGKDAYLVALGERVRHLRAMRGMTRK
                     ALARGADVSERHLANLEGGMGNVSILVLSQIAQAFHCPLAELVGDVTTSTPEWLLIRE
                     LLEHRSEQDLQRAREALSDLFGGQPAAAHKSAMRQIALIGLRGAGKSTLGRMLAEDLG
                     YAFVELSAEIERIAGCGILEIHSLYGPSAYRRYERRALEESLQLYPDMVLATPGGLVS
                     EPATMSLMLSHCYTVWVKASPEDHMGRVMAQGDFRPMAGNNEAMADLKRILASREPFY
                     SKADYICLTSHVGLEACFQGLRDHVRQLAGLSV"
     gene            4424..5992
                     /locus_tag="VFR20_01635"
     CDS             4424..5992
                     /locus_tag="VFR20_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEU0229249.1"
                     /translation="MTACPSRLNYAQYLATLNAGRAGKVAYDDGHRRLTYGQLAERNA
                     RFAGLLRELGLRREERAMLLMHDTVDWPVAFLGALHVGVVPVAANTLLTPDDYAYMIT
                     HSRSQAVFVSGALLGTLHDALSRCDHEVRHIIVSDPQPQQPTLPNLAYHVLEDGLATT
                     PTVPAVDTSADEIAFWLYSSGSTGQPKGVVHTHGNLWHTVELYGKPVLGIQEDDIVFS
                     AAKLFFAYGLGNALTFPLSVGASVVLLPGRPTPEAVFQRLTDFKPTLFCGVPTLYASM
                     LASTALPARDQVALRICASAGEALPKDLGERFTAHFGCHILDGIGSTEMLHIFLSNQD
                     TDVHYGTTGKPVPGYELELRDEHGTPVPAGVTGDLYIKGPSAALMYWHNNAKTRGTFL
                     GDWLKSGDKYVRDEAGYYTYAGRSDDMIKVSGQYVSPVEVEGVLVEHESVLEAAVVGL
                     PNADGLVKTTAFVVLRPGQEPSDQIRTEIQAYVKTHLAPFKYPRQIRFIDELPKTATG
                     KIQRFRLRQMEHAT"
BASE COUNT         1144 a         1954 c         1788 g         1114 t
ORIGIN      
        1 cgcttttcca tgactttgtt ccagcgcccg atgcccgcca cggaatcgcg gatgaaatca
       61 tcccgcaaca cctcgttcaa ggcattgagc atgggcacct cgatctcgcg cagtccgccg
      121 ttggcgacat ccagcacgcg gtaggtgtca tttttaagct gatggtcgtc ggtgcgcttg
      181 ccttcttcgt agcgaccttt cagcccggcg ccatagaaga tggcggcgtt cgatgactgg
      241 tcggcgccga acagatcaat ggtcacgctg taatggaaat tcaggtagcg ctggattgtg
      301 ggaaggtcga tggcgcccgc agcgcgcacg tcggccggat cgtcggtctt gatttcattc
      361 atgacatcgc aggtgcgctg cagcacgcgc gacaccccgg actcacccac gaacatgtga
      421 tgcgcctctt cggtcagcat gaagcgcgtg gtgcgcgcca acggatcgaa tcccgactca
      481 gccaaggcgc acaactggaa cttgccatcg cgatcggtga aatacgtgaa catgtagaac
      541 gccagccaat ccggcgtctt ctcattgaaa gcccccagaa tgcgcgggct gtcctggtcg
      601 ccggaattgc gctgcaacag cgcatcagcc tcttcgcggc cgtcacggcc gaagtagcga
      661 tgcagcaaat acaccatggc ccacaagtgg cgcccttctt ccacgttgac ctggaacagg
      721 ttgcgcaagt cgtagagcga cggcgcggtc aggccaagat ggcgctgctg ttcgaccgag
      781 gccggctcgg tgtcgccctg ggtgacgatg atgcggcgca ggttggcgcg atgctcgccc
      841 ggcgcttcct gccacacatc gcggcccaga tgttcaccaa aatgaatctt gcgattgccg
      901 tcctgcggcg tcaggaaaat gccccagcga tagtcgggca tgcgggtgta gtcgaaatgc
      961 gcccagccat cgggctgcac gctggtggcc gtgcgcaggt acacatcgtg gccctgcgcg
     1021 ccttccggcc ccatctcgtt ccaccattgc aggtaattcg gctgccagtg ctcgagcgcg
     1081 cgctgtaggg cgcggtcttc cgacaggttc acgttgttgg ggatcttctc tgaatagttg
     1141 atgccggaca tgtcgggttc tccgtgatga atgtgcagga tcagacgcgg ttccagtcga
     1201 aactgggctg ctcgcctttt ccgtaaagct tcagcgctcc tttctcgccc accgcgttgg
     1261 ggcggttgaa tatccagttc tgccaagcgg tcaaccgccc gaacacgcgg gtctccatgg
     1321 tttcctgtcc gccgaaacgc aggttcgcct cgagcccggt caacgcatcc ggcgacaagg
     1381 cgcggcgttc ttcaagcgcc aggcggattt cgtcgtccca atccaggctg tcgggcgaca
     1441 gcgtcaccag gccgatctct tcggcctccc gggcggacag gaagcgcccc agcgtggcgc
     1501 gcaccgcgtc gagcgctggc gcctcgtcgt agaaacggtg cgccagccgg ctttgtccgt
     1561 tcaccatggg gtagtcacca aaattcgatt cgcccaccgc gatccgggcg ggtgcgtcgg
     1621 catcggggtc gtccagcatg tacgagcggt cgcaggccaa cgccagctcc agcaaggtac
     1681 caacgaaaca ggactccggt tcaatcaggg cgtacaggga tcgcgacgtg acgtccagcc
     1741 gcgccagggt acggcgcagc atgcccacgg tctcgcgaac gaaccagtga tgtccgaagc
     1801 gcttgagcgt ggcatcgcac gccagcacgc gcgcggcatc accctcggtc ttgaacaccc
     1861 aggtgccgat gtctagctca ttggtgcgca tgtgcaggat ggcgtcatcc agttcccgcg
     1921 ccatgcgcag cggccaccat gacgcgccct gcgcgagaat gccgtcgatg tcgtccggcg
     1981 tgtccgcatc aggggcgcgc acggtccagg tcgccaggcg ttccgcacga tcgatgcaga
     2041 cgtccacgca atggtaggac agaccgtcca cacccgccga acgctccaat ggcgtcaacg
     2101 ccacaccttg ttcattggcc ggacggtcac tgcggtcggc cagttccagc gcccgggcat
     2161 tcaccccttc tgtgaattgc gcgggcttga tgagttcatc aactagccgc caatccttgg
     2221 cgcgctgccc tcgcacaccc tcgaccaggg tgcagaaaat atcggcgcga tcatggcgca
     2281 ccttgcgctt gtcggtgacg cgggtcagcc cgccggtgcc tggcaggacc cccagcagcg
     2341 gcacctccgg caacgccacg gccgatgaac ggtcgtcaat aagcaggatt tcgtcgcagg
     2401 ccagcgccaa ttcatagccg ccgccggcac aggcgccgtt gagggcggcc acgaatttca
     2461 acccgctgtg acggctggag tcttcgatgc cgttgcgggt ctcgttggtg aacttgcaga
     2521 agttgacctt ccacgcatgc gacgacaggc ccagcatgaa aatattggcg cctgaacaga
     2581 acacccgatc cttcaggctg gtgacgacca cggtgcgtac ctcggggtgc tcgaaccgga
     2641 tgcgctgcaa ggcgtcgtag agttcgatgt ccacgcccag gtcataggag ttgagcttga
     2701 gcttgtaacc cggccggatg ccgccgtctt catcgacgtt catggccagc gtggccacca
     2761 cgccgtcaaa cgacaagacc cagtgccggt acaggccggg atgggtgcgg aaatcaacca
     2821 gcggcatggg ttggctcatg acatctcctg ttctgaatga tgaaaaataa ttcatcgtca
     2881 atatttaaat gcataataat gcacttaaat atcatcgtca acaggaaatg tattttgatg
     2941 catttttttg ttggcgcttc tgattcttta ggcgctgtcc accggggctt ggggcagggc
     3001 cggcacggcg tgcttgatcc ggatccaccg cgtccaggcg ggcgtcgggc tgaactcgca
     3061 ccggcaccag ccctatggcc tggtgccggt gctcagacac cgcccgacga cttcccccgc
     3121 ccggacgcgg gggatccggc gcacgcctta cgccggccct gccccaagcc ccggtggaca
     3181 gctcgcggtg atgacttgcg ggggaggtga atggcgccgg ctttcatggg cggtgccggc
     3241 tttcgcgcgg cgggggtgtg tggattgcgg tcccgtgatt gcggtcccgt tttcgcgcgc
     3301 gcaatcatgt ccttgttcga ccggaaagcc ctaaaccgag agtcccgcca gttgtcgaac
     3361 gtgatccctc agcccttgga aacatgcctc cagcccgaca tggctggtca ggcaaatgta
     3421 atcggccttg gagtagaacg gctcgcggct cgccagaatg cgtttgagat cggccatggc
     3481 ttcgttgttg ccggccatgg ggcggaaatc cccctgcgcc atgacacgcc ccatgtggtc
     3541 ttccggagaa gccttgaccc acacggtgta gcaatgcgag agcatcaggc tcatggtggc
     3601 gggctccgac accagcccgc caggcgtggc gagcaccatg tccgggtaaa gctgcaagga
     3661 ctcttccagg gcccggcgct cgtagcgccg gtaggcgctg gggccataca ggctatggat
     3721 ctcgagaatg ccgcagcccg cgatgcgctc gatctcggcg ctgagctcga caaacgcata
     3781 acccagatcc tccgccagca tgcggcccag tgtggacttg cccgcgccgc gcagtccgat
     3841 gagcgcgatc tggcgcatgg cgcttttgtg cgcggccgcc ggctgtccgc cgaacagatc
     3901 ggacaaggct tcgcgcgcgc gttgcaagtc ctgctcggag cgatgctcca gcaattcgcg
     3961 gatcagcagc cattcgggcg tggacgtggt gacatcaccc accagttcgg ccagcgggca
     4021 atgaaaagcc tgggcgatct gcgacagaac caggatggag acattgccca tgccgccctc
     4081 cagattcgcc agatgacgct ccgagacatc cgcgccgcgc gccagcgcct tgcgggtcat
     4141 tccgcgcatc gcccgcaggt gccgcacccg ctcacccagg gcgacgagat aggcatcctt
     4201 gcctggatcg acgttgtccg gctcgctgtg cggcgggagt tcgaatgctt tatttttcat
     4261 ccagaataac cctagccgtc atggatacat gcattatatt gcttgacagc attttcaaaa
     4321 agccctaatt tttaaccaag atctgcaaaa ttcttcagga acgccctcga cctgataaac
     4381 agcccgatcg gggccaccag tataaagaac aaccgagaca gccatgaccg cttgccccag
     4441 ccgactgaat tacgcgcaat atctcgccac gctcaacgcc ggtcgcgctg gcaaagtcgc
     4501 ttacgatgac gggcaccgtc gcctgacgta tggccaactg gccgaacgca acgcccgctt
     4561 cgccggcttg ttgcgcgaat tgggcctgcg ccgtgaagag cgcgcgatgc tgctgatgca
     4621 tgacacggtg gactggcctg tcgccttcct gggcgcgctg cacgtgggtg tggtgcccgt
     4681 cgccgccaac actttgctga caccggacga ctacgcgtac atgatcaccc acagtcgcag
     4741 ccaggcggtg ttcgtgagtg gcgcgctgct aggcacactg catgacgcac tgtcacgctg
     4801 cgaccatgaa gtccgacaca tcatcgtctc cgacccccag ccacaacaac cgaccctgcc
     4861 caacttggcg taccacgtgc tggaggatgg tctggcaacg accccaacgg tgcccgcagt
     4921 cgacaccagc gccgacgaga tcgcattctg gctgtactca tccggctcca ccggtcaacc
     4981 caaaggcgtg gtccacaccc atggcaacct ttggcacacg gtcgaactct acggcaagcc
     5041 ggtgctgggc atccaggaag acgacatcgt gttctccgcc gcgaaactgt tcttcgctta
     5101 cggtttgggt aacgcgctga cattcccgct atcggtgggt gcatccgtcg tgctgttgcc
     5161 cgggcgcccg acccctgaag ccgtgttcca gcgcctgacc gacttcaagc ccaccctatt
     5221 ctgcggcgtg cccacactgt acgccagcat gctggcctcg accgcgctgc ccgcgcgcga
     5281 ccaagtcgcc ctgcgaatct gcgcctcggc gggcgaagcg ttgcccaagg atctgggtga
     5341 acgtttcacc gcgcatttcg gctgccacat actggacggc atcggttcca ccgagatgct
     5401 gcatatattc ctgtcgaacc aggacacgga cgtacattac ggcaccaccg gcaagcccgt
     5461 tccgggctac gaactggaac tgcgcgacga gcacggaacg ccggttcccg caggcgtcac
     5521 cggcgacctg tacatcaagg gtcccagcgc cgccctcatg tattggcaca acaacgccaa
     5581 gacccgtggc accttcctcg gcgactggct caaaagcggc gacaagtacg tgcgcgacga
     5641 ggccggctac tacacctatg cggggcgcag cgacgacatg attaaagtca gcggccaata
     5701 cgtctcgccc gtcgaggtgg aaggcgtgct ggtcgaacac gaatccgtgc tcgaagccgc
     5761 cgtcgtgggc ttgcccaacg ccgacggcct ggtgaagacc accgccttcg tggtgctgcg
     5821 ccccggtcag gaacccagcg accagatccg cacggagatc caggcctacg tcaagacaca
     5881 cctcgcgcct ttcaagtatc ctcgccagat ccgcttcatc gacgaactcc cgaaaacggc
     5941 caccggcaag atacaacgtt ttcgcctgcg acaaatggaa cacgcgacat gaccgcatca
//
LOCUS       DASTNK010000023        16973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_939497, whole genome shotgun sequence.
ACCESSION   DASTNK010000023 DASTNK010000000
VERSION     DASTNK010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 16973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16973
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939497"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VFR20_01640"
     CDS             complement(<1..456)
                     /locus_tag="VFR20_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HEU0229250.1"
                     /translation="MAHYEFNMPVTEAQARQLHVNDTVTLQKTLFGIRDATLIHMYDR
                     GRITRLDLSGHAVIHTAPNVKKVSKSAEFPCGYAPVCIGTTTSDRMERFTRPLMEKNG
                     VRFVIGKGGMREDSLAAFQQFGGAYLVISGGVAALETTWIEQIEDVDLDD"
     gene            complement(459..1334)
                     /locus_tag="VFR20_01645"
     CDS             complement(459..1334)
                     /locus_tag="VFR20_01645"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEU0229251.1"
                     /translation="MEVDLHAVEEAAKELYIRALKVLPPDIKEGFRRLVLEEQSSRAQ
                     QMLATMTTNIKVAESTNNLLCQDTGVPVYYVTIGRNVKVDGARLIQAIRRGCERATRE
                     HPLRSSIVHPLTRRNNQNSCGIEVPVISVDFTDESDVLKILMVPKGSGAENNSYLKMA
                     IPAEGVNAIKTFVIECVLAAGGKTCPPTIVGVGIGGTSELCMALSKRACIRALGSSCS
                     DPDGAVLEKQLSEAVNKLGIGPQGLGGDSTSFAVHIELAATHITMNPVAVNMQCHSAR
                     RASATFTPENVSYGF"
     gene            complement(1420..1779)
                     /gene="sdhC"
                     /locus_tag="VFR20_01650"
     CDS             complement(1420..1779)
                     /gene="sdhC"
                     /locus_tag="VFR20_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEU0229252.1"
                     /translation="MKRNDNRARQHPSYWAFAVHRISGILLALFIPLHFWVLSLALKG
                     EAALNGFLVFTDTPVFRFGEWVLVILLSLHLMGGVRLLLIAFRPASPPALLKGWIAGA
                     VGVAAVAGLAFILSLTT"
     gene            complement(1776..2126)
                     /locus_tag="VFR20_01655"
     CDS             complement(1776..2126)
                     /locus_tag="VFR20_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HEU0229253.1"
                     /translation="MKTSVAAVRARRWYWQHVSAMVLAVCVAVHIVIIVYAMHGGLTG
                     AEILARTHDNWTFGIFYAVFVIACAVHVPAGVANVLEEWVGLRVATSDVISKLFGLLI
                     LVLGLQAVYAVILS"
     gene            complement(2123..2842)
                     /locus_tag="VFR20_01660"
     CDS             complement(2123..2842)
                     /locus_tag="VFR20_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEU0229254.1"
                     /translation="MPAGLLVVKVWRGREQGRLEQYEVPRRASQTVLDVVTYIQRHLD
                     HTLSYRFSCRVGMCGSCAMNVNGVARWTCRTHVSKVVKGDTLELTPLSNLPVIKDLTT
                     DMSTFFDKWARAKGQFHGGKTRHDGFAIVDPNSKERIAAADGTECIGCGVCYSSCDVV
                     TWKPDFLGPAALNRAWTLVNDVRDVEQKERLEAVSGDTGCHSCHTQGSCTERCPKHIA
                     PSFSIAGLKVLSAKAAIRGKL"
     gene            3270..4613
                     /gene="gdhA"
                     /locus_tag="VFR20_01665"
     CDS             3270..4613
                     /gene="gdhA"
                     /locus_tag="VFR20_01665"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEU0229255.1"
                     /translation="MKYTSFEDFIRHAESRNPGQPEFLQAVTEVTESLWPFIQRTPRY
                     MEAGLLDRLVEPERVIMFRVSWVDDHGDVRVNRGYRVQHSSAIGPYKGGLRFHPSVNL
                     SILKFLAFEQTFKNALTTLPMGGGKGGSDFDPKGKSPGEVMRFCQAFVTELFHHVGAH
                     TDVPAGDMGVGGREIGFMTGMMKKLTNRADCVFTGKGLTFGGSLIRPEATGYGTVYFA
                     EEMLKRRGSSFDGLRVSVSGSGNVAQYAVQKAMELGARVITVSDSSGTVVDQEGFTEE
                     KLAELMEVKNHLYGRVSDYAKRVNVGFHPGIRPWHVPTDVALPCATQNELDGGDARTL
                     VDNGVACVAEGANMPSTIEAIKVFEGANVLYAPGKASNAGGVATSGLEMSQNAMRLSW
                     LREEVDNRLQTIMHSIHAACVEHGRREDGSISYVNGANVAGFVKVADAMLAQGVI"
     gene            4616..5458
                     /locus_tag="VFR20_01670"
     CDS             4616..5458
                     /locus_tag="VFR20_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0229256.1"
                     /translation="MRTQPFGPTQRQVSILGQGTWYIDDAHRTSAVAALRLGLDSGMN
                     HIDTAEMYGSGAAERVVGEAIQGRRDDVFLVSKVLPSNAARRGVREACERSLKALGTD
                     RLDCYLLHWRGSVPLGETFAAFEDLVAAGKILSFGVSNFDVADLEEALAVIGPGKLAC
                     NQVLYHLQERAIEHTVVPWCHRHDVAVVGYSPFGHDDFPDPDTPAGQTLESIAQAHNA
                     TAHQVALAFLTREPGLFTIPKSSRPDHTRINTGGDLTLSDSDVARLDAAFPRGPLPAY
                     LPMI"
     gene            complement(6140..7303)
                     /locus_tag="VFR20_01675"
                     /pseudo
     CDS             complement(6140..7303)
                     /locus_tag="VFR20_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
     gene            7559..8764
                     /locus_tag="VFR20_01680"
     CDS             7559..8764
                     /locus_tag="VFR20_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0229257.1"
                     /translation="MRRYRILPRLGQVAVATMMLALAGCKEQAPPPPPPFEVGVVTVH
                     PQSVAVTTELPGRTSPYLVAQVRARVTGIVLNRDFREGSRVKVHQSLFKIDAAPYKAA
                     LDAAKATLQKAEANLTSTTLQANRYKLLVASHAVSSQDYANAVAAQKQAAADVAGGKA
                     SVEIARINLDYTDVTSPIAGIIGASDVTPGAYVQSSAATLMATVQQIDPIYVDLTQST
                     TDVMRMRGELADGRMQSAGPNEAKVQLVLDNGSVYPLAGRLEFTDITVDPSTGSVKLR
                     ALFPNPQHLLLPGMFVRARLREGINDRAILVPEAGVTHDAKGAPTALVVGPDDKVELR
                     NLVANRIHGADWVVDSGLNPGDRVIVAGIQKVRPGMTVRAVEQPTRSMAASTARGPAS
                     MRLASVASR"
     gene            8780..11947
                     /locus_tag="VFR20_01685"
     CDS             8780..11947
                     /locus_tag="VFR20_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0229258.1"
                     /translation="MVNFFIDRPIFAWVIAIVLMLAGALSITTLPVEQYPSIAPPSVQ
                     IRATYAGASAKTVEDTVTQVIEQQMNGLDHLLYMSSTSDDSGQARITLTFANGTDPDT
                     AQVQVQNKLQLATPLLPQAVQQAGISVTKSNSSFLMVMAFVSTDHSMTKYDLANYLVS
                     HIQDPISRVNGVGTVTVFGTQYAMRIWLDPNKLTRVGLTPVDVVNALKGQNVQIAAGQ
                     LGGTPAVSGQALTATIKEATLLRTPAQFGNVLLKVNPDGSQVRIKDVARVELGAENYT
                     TNTHYDGVPAAGLGVQLATGANALQTANAVRAKVEELSHYFPHGLEVKYPYDTTPFVK
                     ISIEDVVITLLEGIVLVFLVMYLFLQNFRATLIPTIAVPVVLLGTFGTMAAVGFSINT
                     LSMFGLVLAIGLLVDDAIVVVENVERVMAEEGLSPRDATRKAMGQITGALVGVALVLS
                     AVFVPMAFSGGAVGAIYRQFSLTIVAAMVLSVLVALILTPALCATILAPIPKGHHLEK
                     TGFFGWFNRAFDNSRQKYGTGVERVIHRPRRWLLLYGLLIIAMGVLFLRLPTSFLPNE
                     DQGMMMIQVQTPPGATMQLTEKALEDVRRYLVDDESAVVESTFEISGFNFAGRGQNSG
                     LIFVRLKDWSQRRAANDKVQALVRRIAEHFATYKYAEVIPINPPPIHELGTASGFDFE
                     LEDRGGLGHDALMAARNELLVKAASDPVLARVRANGLNDQPEFTVNVDREKAGALSVP
                     VADIDQTFSIAWASAYVNNFLDTDNRIKRVYVQAAAPYRMNPQDVNLWYVRNTEGKMV
                     PFSSFATGSWAFGSPKLERYNGVSSMEIQGATAPGQSTGQAMAEMEKIARTLPAGIGY
                     EWTGISYQEQLSGSQAPILYAISILVVFLSLAALYESWSIPFSVIMVVPLGIIGALLA
                     ATGRGLSNDVYFQVGLLTTVGLSAKNAILIVEFAKALQEQGMSAAAAAMEAARLRLRP
                     ILMTSMAFILGVLPLAISNGAGSASQHAIGTAVIGGMLTATFLAIFMIPMFFVVIRDR
                     FSKNRRARGQGNETAALPPSPAGEP"
     gene            complement(12027..13367)
                     /locus_tag="VFR20_01690"
     CDS             complement(12027..13367)
                     /locus_tag="VFR20_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0229259.1"
                     /translation="MSLYAKLQRRAADGNPVRIALIGAGKFGAMYLAQIPRTPGVHLV
                     GIADLAPDRARANLARVGWGPERLAAASARQALSTGSTWVTDDWQALIKLPEVDVVVE
                     CTGNPIAAVEHCLAAFEQGRHVVNVTVEADAFCGPLLARKAAEAGVVYSLAFGDQPAL
                     ICDLVDWARTCGFPVVAAGRGHKWLPHFAESTPDTVWPNYGLTPEQAARGGLNPKMFN
                     SFLDGSKPSIESTAVANATGLDVPSGGLRYPPASVADIPYVTRPVSEGGVLEHKGMVE
                     VVSSLEADGRAIPYDIRMGVWVTVEAETDYIKHCFEEYNVHTDPSGRYFTLYKRWHLI
                     GLEVGMSVASVALRGEPTGVATCWNADVVATAKRDLVVGDMLDGEGGYTVWGKLQPAK
                     KSLAMGGLPLGLAHGVKMIRPVAKGQCVTWADVATDTSTPAYRIRRELEAMAKA"
     gene            complement(13486..15645)
                     /locus_tag="VFR20_01695"
     CDS             complement(13486..15645)
                     /locus_tag="VFR20_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004629 - phospholipase C activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase C, phosphocholine-specific"
                     /protein_id="HEU0229260.1"
                     /translation="MPDPGRRAFLKSAAVAAGSLAAFEALPASICKALAIPAHAGTGT
                     LDDVAHIVVLMQENRSFDHYLGHLSGVRGVNDRFPARQPDGRTVWQQPQGGFSSGTIA
                     PFHLDTRTTNAQCIGDLDHGWRRTHLAIDGGRQDGWVRYKTAMTMGYHLRRDIPFHYA
                     LADAFTVCDHYFCSLPGPTHPNRMFLMTGTVDPSGKGGGPLVNNHDPVSFPSEPGFTW
                     STYPERLEEAGISWRIYQEGTNGHDAYHGNFGLNVLANFKRYIEAPPGSALHERAMTS
                     RTLTQFAQDVKRGELPQVSWLLPPAAFSEHPRWTPAYGATYIDRVLTALTANPAVWSR
                     TVLFIPYDENDGFFDHIVPPQPPVGGQGKSTVDVRDELHTYVDRSRRPVRPADGLPYG
                     LGSRVPMLVVSPWSRGGVVCSDVCDHTSVIRFIERRFGVAEPNISAWRRTVCGDLVSS
                     LDFSRRDIARPSLPDTSDYRARADSQCRIATYPEAPPPGELPKEAQEPGTRPTRPLGY
                     DLHADVLIETRAGASSRLKLVLRNSGARGAHVWARANDSRQPPRSYTVAAGKSLSDDL
                     PVLPSPNPIVRYDWTLYGANGFVRFFQGTFARSGRQVDPPLVVRVGRAQMGLSPDPLS
                     DVSSLDDLSLSLENTGPGSLILTLADMAYGQPEQKIVLGAGARRTVSWPVATSHHWYD
                     VGVTVAGFAGYLRRCAGHLETGHDSRTDPAALAPVWS"
     gene            complement(15668..>16973)
                     /gene="fusA"
                     /locus_tag="VFR20_01700"
     CDS             complement(15668..>16973)
                     /gene="fusA"
                     /locus_tag="VFR20_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU0229261.1"
                     /translation="FKNRGVQALLDAVIDYLPSPVDIPPVAGHDEQDKPVLRHARDDE
                     PFSALAFKIMTDPFVGHLTFFRVYSGVMRSGDAVYNSVKGKKERVGRLLQMHANHRSE
                     LTEVEAGDIAAAVGLKEVTTGETLCDPAHVITLERMVFPEPVISQAVEPKTKTDQEKM
                     SLALNRLAQEDPSFRVSSDEESGQTIISGMGELHLEVLVERMRREFGVEATVGKPQVA
                     FRETIRKTCESSEGKFIKQSGGRGQYGHVELKLEPLPPGTGFQFVDAIKGGVVPREFI
                     PAVEKGIVDTLPSGVLAGYPVVDVKATLFFGSYHDVDSNENAFRMAASIAFKDGCRKA
                     GPVLLEPMMAVEVETPEENMGDVMGDLSSRRGQIQGVDDIPGGAGKAIRAEVPLAEMF
                     GYSTTLRSLSQGRATYTMEFRHYSEAPQNVAEAVIASRKVGS"
BASE COUNT         3329 a         5502 c         5139 g         3003 t
ORIGIN      
        1 gtcatccaga tcgacatcct cgatttgttc tatccaagtg gtctccagcg cagcgacgcc
       61 cccggatatg acgagatacg ctcccccaaa ctgctgaaac gcggccagag agtcttcacg
      121 cataccccct tttccgatga cgaaccgcac cccatttttt tccatcaatg ggcgcgtgaa
      181 ccgctccata cggtcggagg tggtcgtccc gatgcagaca ggagcatatc cacacgggaa
      241 ctcggcgctc ttggatacct tcttgacatt cggcgcagta tgaatcactg cgtgaccgga
      301 gaggtcgagg cgcgtgatgc gaccacggtc gtacatatga atgagcgtcg cgtcgcggat
      361 gccaaacagg gttttctgca aggtcacggt atcgttgacg tgcaactgcc ttgcctgggc
      421 ttccgttacc ggcatattga attcataatg agccatgatc aaaatccgta agagacgttt
      481 tctggggtga atgtggccga tgcgcggcgc gcggaatgac actgcatgtt caccgcgacc
      541 gggttcatcg tgatgtgcgt ggcggccaac tcaatatgaa cggcgaatga ggtcgagtcg
      601 ccaccaaggc cctgcgggcc aatccccagc ttattgaccg cttccgacag ctgtttttca
      661 agcacggcac cgtcgggatc ggaacaggac gaacccaacg cgcggatgca ggcacgcttg
      721 gaaagcgcca tgcatagctc agatgttccg ccaatgccca cacccactat tgttggcgga
      781 cacgtcttgc cacctgcggc gaggacacat tcgatgacaa aagtcttgat ggcgttcacc
      841 ccctcggccg ggatcgccat tttcaggtaa gaattgtttt ccgccccgct gcctttcggt
      901 accatcagga tcttcagcac atcggactca tcggtgaagt caacactgat gaccggcacc
      961 tcgatgccgc aggaattctg attgttccga cgagtcagcg gatgcacgat agatgaacgc
     1021 agcggatgct cgcgcgtcgc ccgctcgcac cctcgacgaa tggcctgtat caacctcgcg
     1081 ccatcgactt tcacgttgcg accaatggtc acgtagtaca cgggcacacc tgtgtcctga
     1141 cacaacagat tattggtgga ttccgcaacc ttgatgttgg tggtcatggt cgccaacatc
     1201 tgctgagcgc gcgatgattg ctcttccagc accagccgcc tgaaaccctc cttgatatcg
     1261 ggcggtaata ccttcaatgc ccggatatag agctctttcg cagcctcttc gaccgcatgc
     1321 agatcaactt ccacagggat acctccaaat ttcttccgtt tcgacggcct aggttatgaa
     1381 atccaacagt taccatagct ctcgccgcag ccgacaaaat caggtcgtca aagataaaat
     1441 gaacgccaag ccggccaccg ccgccactcc gaccgcgccc gcgatccaac ccttgagcag
     1501 tgcaggcggc gaagccggtc ggaaagcgat cagcagcaac cgcacgccac ccatcaaatg
     1561 cagggacagc aggatgacaa gcacccattc gccgaaccgg aaaacaggcg tatcggtaaa
     1621 caccaaaaac ccgttgagtg ccgcctcacc cttgagtgcg aggctcagca cccaaaagtg
     1681 cagcggtatg aacagggcca acaagatgcc tgagattcga tgcacggcaa acgcccagta
     1741 agacgggtgc tggcgcgcac gattgtcgtt gcgtttcatg acagaatcac cgcgtaaacg
     1801 gcctgtaggc ccagaacaag aatgagcagc ccgaacaatt tggaaatgac atctgaggtc
     1861 gccacacgca ggcccaccca ttcttccagc acattggcga caccggctgg cacatggacc
     1921 gcacaggcga ttacaaatac tgcgtaaaaa atgccgaacg tccagttgtc gtgcgtgcgc
     1981 gccaggattt ccgcccccgt cagtccacca tgcatcgcat acacgattat cacaatgtgg
     2041 actgccacac acacggcaag taccatcgcg ctaacgtgtt gccaatacca acgacgcgca
     2101 cggacggcgg caacactcgt tttcatagct tgcctcggat cgccgctttg gcgctgagaa
     2161 ccttcaaacc cgcgatactg aagcttgggg cgatgtgctt ggggcaccgt tccgtacacg
     2221 aaccttgcgt atgacaggaa tggcagccag tgtcacccga gactgcctct agacgctcct
     2281 tctgctcgac gtcacgcacg tcattgacta gcgtccaggc gcggttcaat gctgccgggc
     2341 cgaggaaatc gggtttccat gtcaccacgt cgcaagacga ataacagacg ccacacccaa
     2401 tgcactccgt accatcagcc gccgcgatac gttctttcga gttcggatcg acgatggcaa
     2461 aaccgtcatg tcgtgttttc cctccgtgga actgtccttt ggcacgcgcc catttgtcga
     2521 aaaatgtgct catgtcagtg gtgagatctt tgatcacagg caaattggac agcggggtaa
     2581 gctcaagcgt gtcccccttg accaccttcg aaacatgtgt gcggcacgtc cacctggcaa
     2641 cgccgttgac gttcattgcg cacgacccgc acatgccgac ccgacaagag aaacgatacg
     2701 aaagcgtatg gtccagatgg cgctggatat aggtgacgac gtcgagtaca gtctgactcg
     2761 cgcggcgcgg tacctcgtac tgttccagac gaccctgctc ccgtccgcgc cagaccttta
     2821 ctaccagtag acccgcaggc atttcggtac tccataaagg gcaactcgac atcttgtcgt
     2881 acctttattg tcggccgagg aaataaaaga aaaaagctag tatttattcg cagaaaagaa
     2941 agtttttctt gtcgattctc aatcatctaa cacgtgatag cactccaatg tcccgatcgc
     3001 gtctggccca gatgccaatc acctacggaa ccgagaatgg gtaatattcg tgtttatgcg
     3061 cgtttatgct tgtgtgtcgc agaatctttt gagccagcag catgcgcagc attttgggct
     3121 tgacaatata gcgatacccc acctcccccg actgactggg ggatagcccc tcaccgacga
     3181 caccctcctc ccttcccgtc atgccaatac ggccggcgca tacaatgccg tatcgcacca
     3241 gacagactaa aaaagggaga agccaaatcg tgaagtacac gtcgttcgaa gatttcatca
     3301 gacatgccga atcgaggaac ccagggcagc ccgagtttct ccaggccgtg accgaagtga
     3361 cggaaagcct gtggccattc atccagcgca ccccgcgcta catggaagcg ggcctgctcg
     3421 accgtctggt cgaaccggaa cgcgtgatca tgttccgcgt gtcctgggtg gatgaccacg
     3481 gcgacgtgcg ggtcaatcgt ggctaccgcg tccagcacag ttccgccatt ggcccttaca
     3541 aaggcgggct gcgcttccat ccatcggtga acctatccat cctcaagttc ctggcattcg
     3601 aacaaacgtt caagaacgcg ctcaccacgc tgcccatggg cggcggcaaa ggcggctccg
     3661 atttcgatcc gaaaggcaag agccccggcg aggtcatgcg tttctgccag gcgttcgtga
     3721 ccgaactgtt ccaccacgtg ggcgcgcaca ctgatgtgcc ggcgggcgac atgggtgtcg
     3781 gtgggcgtga gatcggcttc atgaccggca tgatgaagaa gctgaccaac cgcgccgact
     3841 gcgtcttcac cggaaagggg ctgacgtttg gcgggtcgct catccggcca gaagccacgg
     3901 gctacggcac ggtctatttc gctgaagaga tgctcaagcg gcgcggcagc agcttcgatg
     3961 ggcttcgcgt gagcgtgtct ggctcgggca acgtggccca atacgcggtc cagaaagcca
     4021 tggaactcgg cgcccgcgtg atcacggtat ccgactcaag cggcaccgtc gtcgaccagg
     4081 aaggtttcac ggaagagaag ctcgccgagc tcatggaagt gaaaaatcac ctctacggcc
     4141 gcgtcagcga ctacgcgaag cgtgtgaacg tcggcttcca tcctggcatc cgtccgtggc
     4201 atgtgccgac ggacgtcgcc ctgccgtgcg cgacgcagaa cgagctcgac ggcggcgacg
     4261 cgcgaacgct cgtcgacaac ggcgtggcat gcgtggccga gggcgcgaac atgccctcga
     4321 ccatcgaagc catcaaggtg ttcgagggcg ccaacgtctt gtacgcaccg ggcaaggcca
     4381 gcaatgcggg cggcgtcgcc acatccgggc tggagatgag ccagaacgcc atgcgcctgt
     4441 cgtggctgcg tgaggaggtg gataacaggc tgcagaccat catgcacagt atccacgcgg
     4501 catgcgtgga gcatggccgt cgcgaagacg gttccatcag ctacgtcaat ggcgccaatg
     4561 ttgccggttt tgtgaaggtg gccgacgcca tgctggccca aggagtcatc taattgtgag
     4621 aacacagcca ttcggcccca ctcagcgcca ggtttccatt ctcggacaag gaacctggta
     4681 tatcgacgac gcccatcgca ccagcgcggt cgcggcgctg cggctggggt tggatagcgg
     4741 catgaatcac atcgacacgg cggaaatgta tggatccggc gccgccgaac gcgttgtggg
     4801 cgaagccatc cagggccgac gcgacgatgt gttcctggta tcgaaggtat taccaagcaa
     4861 cgcggcgcgg cgcggtgtgc gcgaggcatg cgaacgctct ctcaaagccc tgggcacgga
     4921 ccgtctggat tgttacctgc tgcactggcg aggttccgtt cccctgggcg agacattcgc
     4981 cgcgttcgaa gatctcgtcg ccgcgggcaa gatcctgtcg ttcggggtca gcaatttcga
     5041 cgtggccgat ctagaggaag cgctggccgt gatcggcccg ggaaagctgg cctgcaacca
     5101 ggtgctgtac cacttgcagg aacgtgcgat cgaacacacc gtggtgccat ggtgccatcg
     5161 gcacgatgtg gcggttgtgg gctacagccc tttcgggcac gatgacttcc ccgaccccga
     5221 tacgcctgcc ggccagacct tggaatccat cgcgcaggcg cacaacgcca cggcacacca
     5281 ggttgccctg gcgtttctga cccgcgaacc ggggctgttc accatcccca agtcatcgcg
     5341 tcccgaccac acccggatca acaccggtgg cgacctgact ctttccgata gcgacgtcgc
     5401 ccgactcgac gccgccttcc cgcgtggccc gctgcccgcc tacctgccca tgatctgaat
     5461 cacccccggc acgacgcgcc tcggcgcgcg ttctctccat gccggcgggg aacaggctca
     5521 agagggtcca gcccttctcg ggcacccagt cgctatcggg cgtgaaaggc cgcgcatagc
     5581 cctttggggt cagagcgaac aacggcgtca ggcgatcatc gtggctggcg cgatagtcat
     5641 cgtagccgaa ctcggccgtc aacgtggtcg cgtggatggc gccgccctgc acgagagtat
     5701 tgagtagcgc ccagtaggtc gcgtcctccg agaagagagt gacgggtcgc cgtgaggccg
     5761 ccgccttgtg cttgctgctg gcgtactggc gccggccatc cggcaaggca tacgccgtgc
     5821 cgcgtccgta ctggtacacg aaccacgacg tcaccagcgc gttgaagtcc gtacccgaat
     5881 cgatcgccaa caagcgcccc aggccggaca agtcgagatt gtgctgggcg tcctcggaga
     5941 gcgggctgcc atggtacacc ggcagaccct gcatgcgcgc cttggagatc ccatcccagt
     6001 cgctgctgca cagacgcaca ggcacctgtt ggtcctgcaa ggccttggcc acggcttgcg
     6061 ccactggacc cgcgcccaga atcaggaaac cgaccgggtc gggctcggtg acgcccaacg
     6121 ccaccgagac cgggcgggcc gtggcgcttt gcagaatcac cgttccgatg atcacgatga
     6181 aggtcaacgg cagcaattga tgggcgccag gcacttgcag attttccagg cgaatggcga
     6241 acagcgccga tacagcggcc gccacgatgc cgcgcggtgc gatccaggcc agcaacacgc
     6301 gctcgcgcag cgtcaggctg gaaccccagg cggaatacaa aaccttcagt ggccgcgcga
     6361 tcagcagcat ggcgaccagc acgccaggcc ccgtccagcc aagcgcaagc agttcgccga
     6421 aatccatgcg cgccgccaga atgatgaaca gggctgaaag cagcatcaag ctgagggttt
     6481 ccttgaaagc gatgatctcc tccacctgga cgtctttcct gttccccagc cagataccca
     6541 tgatggtcac ggcgagcagg cccgactcat tcaggatggc attggacaca ccgaacaccg
     6601 caaacaccac agccagcatc gtgacattga tcaggtaatc gggtagccag ttgcgccgca
     6661 gcacctcgcc caccacgaat cccaccaaca gccccgcggc gacgccactc gtgaccgtct
     6721 cgccaaacac cgatatcgtg tgcaggaacc cctggctgcc ttgcaaggcg gcgatgaatt
     6781 cgtaggacag caccgccagc aacgccccga tggggtcgat gatgatgcct tcccagcgca
     6841 gggtactggc cacccgcgcc accgggcgca cggtgcgcag caatgggccg atcacggtcg
     6901 ggcccgtcac caccatgatg gcgccgaaca gcaacgccaa ctcccagcgc agacccacga
     6961 tgtagtgtgt ggcaacggtg gtgaccgccc aggtgatcag gatgccggtg ccgaccaggc
     7021 gctgtaccac gcgctccagg ccgcgtatct cgtggatgcg cagtgtcagg ctgccttcga
     7081 agagaatgac ggccaccgac atggatatga agggaaacaa caaatgccca aacaaagcat
     7141 cgggatcgag ccagcccgcg ccaggcccca cggccaaccc cgccagaagc agaaacaaaa
     7201 tggccggcaa ccgcaggcgc caggcgatcc attggcagaa catggcgagg acgccgattc
     7261 ccgccagcgt caggacgaga tcttcattca cgggtttccc catcctcccc aagcatgttg
     7321 tttgattcgt ccattccgat agtaagaatg cagaggtcag ttcctggaac ctggatttgc
     7381 caggcaaggc gcgaaccacc ccttgtttta taacgcggcg aaacagaaat atggttacgg
     7441 cggtatcaag acactgattt tgaaaacaag tggtaactat aatagcctgc cccgaacagg
     7501 cggttgacgc ggattcgggt tcgccgaagg tccattcatc ccgaacagag gttgccgcat
     7561 gcgccgctac cggattctcc cgcgcctggg ccaggtagcc gtggccacga tgatgctggc
     7621 gctggctggc tgcaaagaac aggccccgcc gccaccgcct ccgttcgaag tgggcgtcgt
     7681 cactgtgcat ccacaaagcg tggccgtcac gaccgaactc ccgggtcgta cgtcgcccta
     7741 ccttgtcgcg caagtgcgcg cacgggtcac cggcatcgtg ctgaaccgcg acttccggga
     7801 aggcagccgc gtcaaggtgc accagagtct tttcaagatc gacgccgcgc cttacaaggc
     7861 agcgctggac gccgccaagg ccacgctgca aaaggccgag gctaatctga catcgacgac
     7921 gctgcaggcg aaccgctata agctcctggt cgcgtcccat gcggtcagca gccaggacta
     7981 cgccaacgcg gtcgcggccc agaaacaagc cgccgccgac gtcgcaggcg gcaaggcctc
     8041 cgtcgagatc gcccgcatca acctggacta caccgacgtc acctccccga tagcgggcat
     8101 catcggcgcg tccgacgtca cccccggcgc ctacgtgcag tccagcgccg cgacgctcat
     8161 ggccaccgtg cagcaaatcg accccatcta cgtggacctg acacaatcca ccaccgacgt
     8221 gatgcgcatg cgcggcgaac tggccgatgg gcgcatgcaa tcggcggggc ccaacgaggc
     8281 caaggttcaa ctggtgttgg acaatggctc ggtctacccg ttggcgggca gactggaatt
     8341 caccgacatc acggtcgacc ccagcaccgg cagcgtcaag ctgcgcgcgc tgttccccaa
     8401 cccgcaacac ctccttctgc ccggcatgtt cgtgcgcgcc cgcctgcgcg aaggcatcaa
     8461 tgaccgcgcg atcctggtgc ccgaggccgg tgtcacccac gacgccaaag gggcgcccac
     8521 cgcgctggtc gtaggccccg atgacaaagt ggaattgagg aacctggtcg cgaaccgcat
     8581 ccatggcgcc gattgggtgg tggactcggg cctgaatccg ggcgaccggg tcatcgtggc
     8641 cgggatccag aaggttcggc cgggcatgac ggtgcgggcg gtcgaacaac ccactcggtc
     8701 catggcggct tcgaccgcgc gcggcccggc ttcgatgcga ctggcgtcgg tcgccagccg
     8761 ctgacaccgg gcgaagcaca tggtcaattt cttcatcgac cgccccatct tcgcatgggt
     8821 gatcgccatc gtcctgatgc tggcgggtgc gctgtccatc acgacgctgc cggtggaaca
     8881 gtatccgtcc atcgcaccac cctcggtgca gatccgcgcc acctacgcgg gggcatccgc
     8941 caagaccgtc gaagacaccg tcacccaggt catcgaacaa cagatgaatg gcctggacca
     9001 tctgctctac atgtcgtcca ccagcgacga ttccggccag gcccgcatca cactcacgtt
     9061 cgccaacggc accgatcccg acaccgcgca ggttcaggtg cagaacaaac tgcagttggc
     9121 cacaccgctg ttgccgcagg cggtgcagca ggccggcatc agcgtgacga aatcgaacag
     9181 cagcttcctc atggtgatgg cgttcgtctc gaccgaccac agcatgacca agtacgacct
     9241 ggccaactat ctggtgtcgc atatccagga ccctatcagc cgagtcaatg gtgtcggcac
     9301 cgtgacggtg ttcggcaccc agtacgccat gcgcatctgg ctcgacccga acaagctcac
     9361 gcgcgtgggg ctgacacccg tggacgtcgt caatgcattg aaaggccaga atgtccagat
     9421 cgcggcgggc cagctcggtg gcaccccggc cgtctctggc caggcgctga cggcgaccat
     9481 caaagaggca acgctgctgc gcacgccggc acaattcggc aatgtgctgc tgaaggtcaa
     9541 ccccgacggc tcccaggtcc gcatcaagga cgtcgcccgc gtcgaactcg gcgccgagaa
     9601 ctacaccacc aacacccact atgacggcgt gccggccgcc ggcctgggcg tgcaactggc
     9661 caccggtgcc aacgcgctgc agaccgccaa cgccgtgcgc gcgaaggttg aggagctttc
     9721 gcattacttc ccgcacggcc tcgaggtgaa atacccgtac gacaccacgc cattcgtcaa
     9781 gatttccatc gaagacgtgg tcatcacgct gctcgagggc atcgtgctgg tgttcctggt
     9841 gatgtacctt ttccttcaga acttccgcgc aaccctcatt ccgaccatcg ccgtgcccgt
     9901 ggtgctgctg ggcacgttcg gcaccatggc cgcggtgggg ttctccatca acaccttgtc
     9961 gatgttcggc ctggtgctgg ccatcggcct gctggtcgat gacgccattg tcgtggtcga
    10021 aaacgtggag cgggtcatgg ccgaggaagg cctgtcgccg cgagacgcca cgcgaaaggc
    10081 gatgggacag atcaccggcg cgctggtcgg ggtggcgctg gtgctgtcgg ccgtgttcgt
    10141 gcccatggcc ttctcgggcg gcgcggtcgg cgccatctac cgacagtttt cgctcaccat
    10201 cgtcgccgcc atggtcctgt cggtgctggt cgcactgatc ctcactcccg cgctgtgcgc
    10261 gacgatcctc gctcccattc ccaagggaca ccacctggaa aagaccggat tctttggctg
    10321 gttcaaccgc gcgttcgaca acagccgcca gaagtacggc acgggggtcg agcgcgtcat
    10381 ccaccggcca cgccgctggc tgctcttgta cgggctgctc atcatcgcca tgggtgtgct
    10441 gttcctgcgc ctgcccacgt cgttcctgcc caacgaagac cagggcatga tgatgatcca
    10501 ggttcagacc ccgcccggcg ccaccatgca gctcacggaa aaggctctgg aggatgtgcg
    10561 ccggtacctg gtggacgatg aaagcgccgt ggtcgaatcc acgttcgaga tatcgggatt
    10621 caatttcgcc ggccgcgggc agaattcagg gttgatcttc gttcgcctga aagactggtc
    10681 gcagcgccgc gcggccaacg acaaggttca ggccctggtg cgccgcatcg cggagcactt
    10741 cgccacatac aagtacgccg aggtcattcc catcaatccg ccgcccatcc acgaactggg
    10801 cacggcttct ggtttcgact tcgagctgga ggaccggggc gggctgggcc acgacgcgtt
    10861 gatggcggcg cgcaacgaac tcttggtcaa ggcggcatcg gatccggttc tggcgcgggt
    10921 gcgcgccaat gggctgaatg atcagcctga attcaccgtg aacgtggatc gcgagaaagc
    10981 cggcgcgctg agcgtgccgg tcgcggatat cgatcagacg ttctccatcg cgtgggcctc
    11041 cgcctatgtg aacaatttcc tggacaccga caaccgtatc aaacgggtct acgtgcaggc
    11101 ggccgcgccc taccggatga atccgcagga cgtcaatctc tggtatgtgc gcaatacaga
    11161 ggggaagatg gtgccattct cgtcattcgc gacagggtcg tgggcgttcg gttcccccaa
    11221 gctggaacgt tacaacggcg tgtcttcaat ggagatacaa ggtgcgacgg cgccaggcca
    11281 aagcacgggc caggccatgg ccgagatgga aaagatcgcc cgcacgttgc cggcgggcat
    11341 cggctacgaa tggacgggca tttcatatca ggaacagctt tctggttcgc aggcgccgat
    11401 tctttacgcg atttccattc tggtggtgtt cctgagcctg gcggcgctgt acgagagctg
    11461 gtcgattccg ttctcggtga tcatggtggt gccgctgggc atcatcgggg cgctgctggc
    11521 ggccacgggc cgggggctgt ccaatgatgt gtatttccag gtagggctgc tgaccacggt
    11581 ggggttgtcg gcgaagaacg cgattctgat cgtcgagttc gcgaaggcgc tgcaggaaca
    11641 agggatgtcg gccgcagcgg ctgccatgga ggcggcgcgg ttgcggttgc ggcccatctt
    11701 gatgacgtcg atggcgttca ttctgggggt tctgccattg gcaatcagca atggcgcggg
    11761 gtcggcaagc cagcacgcga tcggaacggc ggtgatcggc gggatgttga cggcgacgtt
    11821 cctggcgatc ttcatgattc cgatgttctt cgtggtgatc cgcgacaggt tctcgaagaa
    11881 ccggcgggcg cgcggccagg gcaacgaaac tgctgcgcta ccaccgtccc cggccggcga
    11941 gccgtaaggt tcgttctcaa ggcggggagc gacggtgact ccgtgattat tggtgtgttc
    12001 acggtttacg tatttcgacg gagagctcag gccttggcca tcgcctccag ttcccgccgg
    12061 atccgatacg cgggcgtgga cgtatccgtg gccacatcgg cccatgtcac gcattgcccc
    12121 ttggccaccg gacggatcat tttcacgcca tgggccagcc ccagcggcag gccacccatg
    12181 gcaagggact tcttcgccgg ctgcagcttg ccccagaccg tataaccccc ttccccatcc
    12241 agcatgtccc ccaccaccag atcccgtttg gccgtggcca ccacgtcggc gttccagcat
    12301 gtggccacgc cggtgggctc gccgcgcagg gccaccgagg ccaccgacat gccgacctcc
    12361 agcccgatga ggtgccagcg cttgtacagc gtgaaatatc ggccggaggg atcggtgtgc
    12421 acgttgtatt cctcgaaaca atgcttgatg taatccgtct cggcctcgac cgtgacccag
    12481 acccccatgc ggatatcgta gggaatggcg cggccgtcgg cctcgagcga tgacacgacc
    12541 tcgaccatgc ccttgtgctc cagcacccca ccctcgctca ccggccgtgt cacatacgga
    12601 atgtcggcca cgctggcggg cggataccgc aggccgccag agggtacatc cagccccgtg
    12661 gcgttggcca cagccgtgct ttcgatggac ggcttggagc catccaggaa actgttgaac
    12721 atcttggggt tgagcccgcc gcgcgccgcc tgctcgggcg tcagaccgta attcggccac
    12781 acggtgtcgg gcgtggattc ggcgaaatgc ggcaaccact tgtggccacg gcctgcggcc
    12841 accaccggaa acccgcaggt gcgcgcccag tcgaccaggt cgcagatcag cgcgggctgg
    12901 tcaccgaacg ccagtgaata caccaccccc gcctcggccg ccttgcgcgc cagcagtggg
    12961 ccgcagaacg cgtcggcctc gacggtgacg ttgaccacgt gcctgccctg ctcgaaggcg
    13021 gccaggcaat gctccacggc ggcgatggga ttgccggtgc attccaccac cacgtcgacc
    13081 tcgggcagct tgatcaacgc ttgccaatca tcggtcaccc aggttgaacc agtgctcaag
    13141 gcctgccgcg ccgacgcggc cgccagccgc tccggccccc accccacacg ggccagattg
    13201 gcgcgcgcgc ggtccggtgc gaggtcggca atgcccacga gatgcacacc cggtgtgcgc
    13261 gggatctgtg ccagatacat ggcgccgaac ttgccggcgc ctatcagggc gatgcgcacc
    13321 ggattgccgt cggcggcgcg ccgctggagt ttcgcgtaca gactcatgac tacccatcct
    13381 tcgatatcaa ggtgaaaacc atccgagcca ggccggacgc caagccccgg ccgatccgag
    13441 ccaggcccag gcctttggga acgtgccggc cgcccccgcc gcgcatcaag accagaccgg
    13501 cgccagcgcc gccgggtcgg tacggctgtc gtggccggtt tccagatggc cggcgcaccg
    13561 gcgcaggtac ccggcgaagc cggccacggt gacacccacg tcgtaccaat gatgactggt
    13621 ggcgacgggc catgacacgg ttcggcgggc ccccgccccc aggacgattt tctgttcggg
    13681 ctggccatag gccatgtcag ccagcgtgag aatgagggat ccggggccag tgttttccaa
    13741 cgacagcgac aaatcatcga gcgacgatac gtcggacaag ggatcggggg acaggcccat
    13801 ctgcgcccgc cccacccgca cgaccagcgg ggggtcgacc tggcgacccg atcgggcgaa
    13861 cgttccctgg aagaagcgca caaaaccgtt cgcgccgtat agcgtccagt cgtagcgcac
    13921 gatggggttc ggggacggca agacgggcag atcatccgac aacgactttc ccgccgccac
    13981 tgtgtagctt cttggcggct gccgtgaatc gttcgcgcgc gcccacacat gcgcgccgcg
    14041 cgcgccgctg ttgcgcaaaa ccagctttag ccgcgaactg gcgccggccc tggtttcgat
    14101 cagtacgtcc gcatgcaggt cgtaccccag cggccgggta ggccgggtgc cgggctcttg
    14161 agcttccttc ggcaactcgc cgggcggcgg cgcctcgggg taggtcgcta tccggcattg
    14221 tgaatcggcc cgcgcccgat aatcgctggt gtccggcaac gatggcctgg caatgtcgcg
    14281 ccgcgagaaa tccagcgacg acaccagatc gccgcacacg gtgcggcgcc aggcgctgat
    14341 gttcggctcg gccacgccga aacgccgttc gatgaaccgg atgaccgagg tatggtcaca
    14401 gacatcggaa cagaccacgc cgccgcgcga ccacggagac accaccagca tgggcacgcg
    14461 cgaccccagg ccgtatggca gcccgtccgc cgggcgcacg ggacgtcgtg aacggtcgac
    14521 gtaggtatgc agctcgtcgc gcacatcgac cgtgctcttg ccttgcccgc ccacgggcgg
    14581 ttgcggcggc acgatgtgat cgaagaagcc gtcgttctcg tcgtagggaa tgaacaacac
    14641 cgtccggctc cacactgcgg ggttggccgt cagcgcggtc agcacacgat cgatataggt
    14701 ggcgccatac gccggcgtcc agcgcgggtg ttcagagaac gcggcgggcg gcagcagcca
    14761 cgacacctgc ggcagttcgc cgcgcttcac atcctgggcg aactgcgtca acgtgcgcga
    14821 cgtcatggcc cgctcgtgca acgccgagcc cggcggcgcc tcgatgtagc gcttgaaatt
    14881 ggccaggacg ttcaggccga aattgccgtg gtaggcgtcg tggccgttgg tgccttcctg
    14941 atagatgcgc cacgagatac cggcctcttc caggcgctcg gggtaggtgg accaggtgaa
    15001 cccgggttcc gaggggaagc tgaccggatc gtgattgttg acgagtggcc cacccccctt
    15061 gccggatggg tccacggtgc cggtcatcag gaacatccga ttcgggtggg tgggcccggg
    15121 caacgagcag aaatagtggt cgcagaccgt gaacgcatcg gccagcgcgt aatggaacgg
    15181 tatgtcgcga cgcaagtggt accccatggt catggcggtc ttgtagcgca cccagccgtc
    15241 ctggcgcccc ccgtcgatgg ccagatgggt gcggcgccag ccgtggtcca ggtcgccgat
    15301 gcattgcgcg ttggtggtgc gggtgtccag atggaacggc gcgatggttc cgctggaaaa
    15361 accgccctgc ggttgttgcc agaccgtgcg accatcgggc tgccgcgcgg ggaatcggtc
    15421 gttcacgccg cgcacaccgc tcaggtgccc aagatagtgg tcgaacgacc tgttctcctg
    15481 catcagcacc acgatgtgcg ccacatcatc gagcgtgccc gtgcccgcgt gggcggggat
    15541 ggccagcgcc ttgcagatcg aggcgggcaa cgcctcgaac gccgccagcg agcccgccgc
    15601 gacggcggcc gatttcagga atgcgcgcct gcccggatct ggcatgggac cctcccgcca
    15661 accgtgttta cgaccccact ttcctggacg cgatcacggc ctcggccacg ttctgcggcg
    15721 cctcgctgta atggcggaac tccatcgtgt aggtggcgcg gccctgcgac agggaacgca
    15781 aggtggtcga atagccgaac atctccgcca gcggcacctc ggcgcggatg gccttgccgg
    15841 ccccgccggg aatatcgtcc acgccctgga tctggccgcg ccgcgacgac aggtcgccca
    15901 tgacgtcccc catgttttcc tcaggtgtct cgacctcgac cgccatcatg ggttcaagca
    15961 acaccggccc cgccttgcgg cagccgtcct tgaaggcaat ggaagcggcc atgcggaacg
    16021 cgttttcgtt ggaatccacg tcgtggtacg agccgaagaa caaggtggcc ttgacatcga
    16081 ccacggggta gcccgccagc accccggacg gcaaggtgtc gacgatgcct ttctcgaccg
    16141 ccgggatgaa ttcgcgcggc acgacgccac ccttgatcgc atcgacgaac tggaacccgg
    16201 ttccgggcgg cagaggttcg agcttgagtt cgacatggcc gtactgacca cgtccgcctg
    16261 attgtttgat gaacttgccc tcgctggatt cgcaggtctt gcgtatggtt tcccggaacg
    16321 ccacttgtgg cttgcccacg gtggcctcca ccccgaactc gcggcgcatg cgttccacca
    16381 acacctccag gtgcagttcg cccatgccgg aaatgatggt ctggccggat tcctcgtcgg
    16441 agctgacccg gaacgagggg tcctcctgcg ccagacggtt gagcgccagg ctcatttttt
    16501 cctgatccgt cttggttttg ggctccaccg cctgggaaat gaccggctcg gggaacacca
    16561 tgcgctccag cgtgatgaca tgcgctggat cgcacagggt ttcgcccgtg gtgacttctt
    16621 tcaaccccac cgccgccgcg atgtcaccgg cctcgacctc ggtgagctcg ctgcgatggt
    16681 tggcgtgcat ctgcaacaga cgcccgacac gctccttctt gcccttgacc gagttgtaga
    16741 ccgcatcgcc cgaacgcatc acccccgaat acacccggaa gaacgtcaga tggccgacga
    16801 acggatcggt catgatcttg aacgccaggg ctgagaacgg ctcgtcgtcg cgggcgtggc
    16861 gcaagacggg cttgtcttgc tcgtcgtgcc ccgccaccgg cgggatgtcc actggcgatg
    16921 gcagatagtc gatgaccgcg tccagcaggg cctgcacccc ccggttcttg aag
//
LOCUS       DASTNK010000024        16494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1310709, whole genome shotgun sequence.
ACCESSION   DASTNK010000024 DASTNK010000000
VERSION     DASTNK010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 16494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16494
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310709"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFR20_01705"
     CDS             complement(<1..200)
                     /locus_tag="VFR20_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit N-terminal domain-containing protein"
                     /protein_id="HEU0229262.1"
                     /translation="MTEIEQTLSSSSHTMVTADHHRRLLLGAASALGAVGVVATAVPF
                     VESMLPSERTKVAAGPVDIDIGG"
     gene            complement(459..1040)
                     /locus_tag="VFR20_01710"
     CDS             complement(459..1040)
                     /locus_tag="VFR20_01710"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEU0229263.1"
                     /translation="MSIRLSIITTRTGDAGVTGLADGSRLDKDAPRIVALGDVDELNS
                     VIGLWRTESLPDGLDDMLGDIQNDLFDLGAELCWPGHAAVSPERVAWLDEAIRRHNEG
                     LAPLTEFILPGGTRGAALAHLARTTCRRAERAVVALHHSEPVGEPLRQYLNRLSDLCF
                     VLARVLNKAAGHADALWRKPGGQPAEAPADTDG"
     gene            complement(1129..1566)
                     /locus_tag="VFR20_01715"
     CDS             complement(1129..1566)
                     /locus_tag="VFR20_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0229264.1"
                     /translation="MREMAEFEKLTDIFHATEASLHRSFFGPQPAANGLIAHTLDDPA
                     VPVAYIMWFHNYSSFLDKRGLYLEDIYVQPAHRGRGLGGLALKHLARLAIALDCGRFE
                     WTVLDWNQNAIDFYVRHGATILHDWRVARVTGDALRALAEMPD"
     gene            1836..2135
                     /locus_tag="VFR20_01720"
     CDS             1836..2135
                     /locus_tag="VFR20_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HEU0229265.1"
                     /translation="MTTTHRWQQALTAALAVTSVYSTAALAQQATASGQVRRVDAAAG
                     KIAIKHGAIADLGLPAMTLVYHAAPSLLAGIKPGDQVTFTAKRENNQYVIVSIKK"
     gene            complement(2217..3548)
                     /gene="hslU"
                     /locus_tag="VFR20_01725"
     CDS             complement(2217..3548)
                     /gene="hslU"
                     /locus_tag="VFR20_01725"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017685724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEU0229266.1"
                     /translation="MSASTMTPGEIVSELDKNIIGQDRAKRSVAIALRNRWRRQQVPE
                     PLRNEIVPKNILMIGPTGVGKTEIARRLAKLANAPFIKIEATKFTEVGYVGRDVDTIV
                     RDLVDISIKQTREVETRRVRTQAEDAAEDRILDVLVSPPRNAQGEPQREENAARQTFR
                     KRLREGKLDSMEIEIEVAQPAAQMEIMAPPGMEEMTEQLKGMFAGIGRDKKKHRKITV
                     KEAFRLLTEEEASRRINEEDLRTTAIANAEQNGIVFLDEIDKIASRQEHSGGDVSRQG
                     VQRDLLPLVEGTTVNTKYGMVHTDHILFIASGAFHLSRPSDLIPELQGRFPIRVELDS
                     LSADDFVSILSKTDSSLTKQYCALLATEEVTLDFQEDGIQRIAEIAFDVNERTENIGA
                     RRLYTVMERLLEDLSYNAASHGAKTITIDAAYVNDKLEEAAANQDLARYVL"
     gene            complement(3575..4108)
                     /gene="hslV"
                     /locus_tag="VFR20_01730"
     CDS             complement(3575..4108)
                     /gene="hslV"
                     /locus_tag="VFR20_01730"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEU0229267.1"
                     /translation="MEQFHATTIICVRRGDQVALGGDGQVTLGNIVIKGTARKIRRLY
                     HDKVLAGFAGATADAFTLQERFEAKLEKHQGHLMRAAVELTRDWRTDRVLRRLEAMLI
                     VADREHTLVLTGTGDVLEPEHGLAAIGSGGAYAQSAARALLDNTDLPPADIVKKSLEI
                     AGDLCIYTNQNHIVETL"
     gene            complement(4180..4623)
                     /gene="dksA"
                     /locus_tag="VFR20_01735"
     CDS             complement(4180..4623)
                     /gene="dksA"
                     /locus_tag="VFR20_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEU0229268.1"
                     /translation="MAGKSAATKVHSQLLTEKEILAMPESDYMNEAQLAFFRDRLKQL
                     EQSILNNAGATTENLRETQFVPDPADRATIEEEHALELRTRDRERKLLKKVQQSITRI
                     DSGEYGWCEETGEPIGIARLLARPTATLSLEAQERREMRQKLYGD"
     gene            complement(4709..5803)
                     /locus_tag="VFR20_01740"
     CDS             complement(4709..5803)
                     /locus_tag="VFR20_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010925725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU0229269.1"
                     /translation="MNAVSKNKMVPVTVLTGFLGAGKTTLLKRILSEYHGQRIAVIEN
                     EFGPESIDNDLLVQDSDEQIIELSNGCVCCTIRGDLMRTLRDMSERREAGTLIFERVI
                     IETTGMANPGPVCQTFFMDDEIADYYRLDAVITIVDAKHGMETLDKQEESQKQVGFAD
                     KLLVSKKDLVNDADYQALRARLVRINPRASITPVNFGDTDLHALLDISGFNLNTILDI
                     DPEFLADEHPDARHDHDHHDHDHDHDGDDDHDHHDGHGHHDHHHAHHDDSIGAFLFRS
                     DRPFDPERLEEFLGGIVQVYGPDLLRYKGILFLKGINRRMLFQGVHMMMGAEPGKAWL
                     SSEKPNTRMVFIGRKLPKEIFTRGLEQCLA"
     gene            complement(6054..6551)
                     /locus_tag="VFR20_01745"
     CDS             complement(6054..6551)
                     /locus_tag="VFR20_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEU0229270.1"
                     /translation="MPGHHTASSQKLADKLGTAEALCEQRGKRLTPIRRKVLEILLRR
                     RRSLKAYELLEEIRAEQPGAVPPTVYRALDFLVEEGLVHRLDAVNAWAACLDAAGEPH
                     DLLIVCTHCGAVAELSDPILSRELAERVAGTGFVLAGHETELRALCRQCLERGAAAHD
                     HPHPH"
     gene            complement(6661..7623)
                     /gene="xerC"
                     /locus_tag="VFR20_01750"
     CDS             complement(6661..7623)
                     /gene="xerC"
                     /locus_tag="VFR20_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEU0229271.1"
                     /translation="MPSVHALPAPMEQWLAHLLTQRRYSVHTVDGYRHDLLHLVACHP
                     DTALERYTESHIRQALARLHAQGGKPRSLARALAAWRGFFQWWAPGAGMASNPASGVR
                     APKIPRSLPKALSVEQVQVLLDRPGQPHSDSIAECRDQAMFEILYSSGLRLAELVGLD
                     WRYVREAGYESHSWIQLDEGEATVRGKGGKTRSVLLGSKAVEATRQWLARRDQLPVAG
                     DPDSGAALFLGMRGRRIAARVVQKQLDQLAVRAGLPVHVHPHGLRHSFASHMLQSAQD
                     LRAVQEMLGHANISTTQIYTRLDFQHLASVYDKAHPRANRRKRD"
     gene            7888..8685
                     /locus_tag="VFR20_01755"
     CDS             7888..8685
                     /locus_tag="VFR20_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEU0229272.1"
                     /translation="MSRFAPSDLPLLSRVDVILTDVDDTLTHKGRLSARTLDAMARLA
                     QAGVRVVPVTGGCAGWCDHIVRAWPVAAVIGESGAFRFSLRQGGALERYFVRPLDELR
                     ADQRRLLEVARRALEAVPAARMAADQDYRLVDVAVDHAQDIAALPPESVQTIIDVFQN
                     AGARARASSIHVNAWFGDHDKASMASHVLAEDFGISSKEERAGRVLFIGDAPNDETLF
                     GASTLSFGVANIAPHLDALVHKPRWVCQTAYGDGFAEMAGHVLAARA"
     gene            complement(8819..9487)
                     /locus_tag="VFR20_01760"
     CDS             complement(8819..9487)
                     /locus_tag="VFR20_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEU0229273.1"
                     /translation="MSDVSFTAQDIADFLLANPEFFQDHADLFANLRVPHPHETRAIS
                     LGERQILTLRTRAKDLEWKLAGLMHNATSNEKISTALNTWCRHMLAEPDATVLPAHMV
                     RGLSDLFDLPTIALRLWNLPGLVDSEFTQDVTESARQYAQALTRPYCGPLDGQEAAAW
                     LGSVPASLAIVALRPDASHDTLGLLVLGSDDPDRFTPDMSTDFLQIINGLASASLLRL
                     KALP"
     gene            complement(9484..10392)
                     /gene="dapF"
                     /locus_tag="VFR20_01765"
     CDS             complement(9484..10392)
                     /gene="dapF"
                     /locus_tag="VFR20_01765"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEU0229274.1"
                     /translation="MGDNLDMNWNFTKMQGAGNDFVVLDGVRQNIDMTPERARALADR
                     HFGIGADQILLVESPSHPDADFRYRIFNADGGEVEHCGNGARCFVRFVHDQGLSGRNP
                     LRAEIHTGLITLNQDTLGHVDVEMGHTSFNPDDVAFDTSGLASRAEGQDTLWALPLDG
                     HGSVDVSLVAISNPHAVQVVDDVQAAPVAIVGPLIESHPRFANRVNAGFMQVLDRHTI
                     ALRVYERGAGETLACGTGACAAVAAGIRRGLLDSPVRVHTRGGDLSVAWNGERVVMSG
                     PAVSVFSGQVDVDALLATQPKDTPQP"
     gene            complement(10395..11288)
                     /locus_tag="VFR20_01770"
     CDS             complement(10395..11288)
                     /locus_tag="VFR20_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU0229275.1"
                     /translation="MTFSKLPVLRGVFSYFGHCATPARLRWGRLLGWLAPRLLRSRVR
                     VVRINLKLCFPHLPASDIDALIHRHFRLLGQSVIDRGLCWFGPRQAILDTVRISGLEH
                     LENLLRDETPIILLAPHFIGLDAAATRLTLFLKESATLYTPQSDPDVDRLVREGRGRF
                     NQVHLISRQDGVRGLIRHLRRGIPVYYLPDMDFGRDGAIFPPFFGVPAATLTTTAQIA
                     GAWKAAVVPIVSRLDLGTGRYHVDVLEPLDAFPGDDGLEVATARLNRVIESWVRSDPA
                     QYYWVHRRFKTRPIEGEPKFY"
     gene            complement(11285..12142)
                     /locus_tag="VFR20_01775"
     CDS             complement(11285..12142)
                     /locus_tag="VFR20_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU0229276.1"
                     /translation="MLLFLFRLLACVPLPLLHGVGRIGGRIVYALPGKYRRRLRDNAR
                     QAGYSDAVFARRAAAQTGAMIMELPKVWLRTEQCLDKIIAGDDDMAIVADAVAQGRGV
                     LCLTPHLGGFEIAARYMAAVHGPMTVMFRPPRKNMLRPIMEQGRNSATLNAVPANMKG
                     VREFVRALRRGGVVGLLPDQAPSSGEGVWVPFFGRPAYTMTLPGKLAAQTHVPVIAAA
                     GERLPHGRGWRIHVVRLPEPLPADPDHQAALVNQTMEALIRRMPDQYLWGYNRYKQPK
                     GAPPPPPST"
     gene            12387..13550
                     /gene="metK"
                     /locus_tag="VFR20_01780"
     CDS             12387..13550
                     /gene="metK"
                     /locus_tag="VFR20_01780"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010925730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU0229277.1"
                     /translation="MANNDFLFTSESVSEGHPDKVADQISDAILDAIFTQDPNSRVAA
                     ETLCNTGLVVLAGEITTTANVDYIQVARDTIKRIGYDNTDYGIDYKGCAVLVAYDKQS
                     PDIAQGVDRTAEEILNQGAGDQGLMFGYACDETPDLMPAPIWYAHRLVQRQSELRKDG
                     RLPWLRPDAKSQVTFRYVNGKPAEVDTVVLSTQHAEEISQKDIHEAVIEEIIKPCFPN
                     GLVTPKTKYLVNPTGKFVIGGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDPSKVD
                     RSAAYAARYVAKNIVAAGLARQCQVQVSYAIGVAEPINITVYTEGTGVIPDDQIARLV
                     REHFDLRPKGIINMLDLLRPIYAKTAAYGHFGRSEPEFSWEATDKVQVLKKAA"
     gene            13633..13890
                     /locus_tag="VFR20_01785"
     CDS             13633..13890
                     /locus_tag="VFR20_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEU0229278.1"
                     /translation="MAERGESVDGAPGAGSVGGAGDRDGSRSLERTDSPCVALCSTLY
                     DDVCRGCGRTVMEVANWVFMSDDEKRRVWERIRAEGYPRRR"
     regulatory      14286..14385
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            14456..15874
                     /gene="ahcY"
                     /locus_tag="VFR20_01790"
     CDS             14456..15874
                     /gene="ahcY"
                     /locus_tag="VFR20_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEU0229279.1"
                     /translation="MNTVTDTVFSDYKIADIGLADFGRREIAIAETEMPGLMATREEY
                     ASAQPLKGARIAGSLHMTIQTAVLIETLVALGAEVRWASCNIFSTQDHAAAAIAAGGN
                     PVFAVKGETLVDYWRYTHKIFEWPEGSSANMILDDGGDATLLLHLGTKAESNPAVLDK
                     PNGEEERVLFAAIRERLAVDSTWYSRRLAQIRGVTEETTTGVHRLYQMSQKGELAVPA
                     INVNDSVTKSKFDNLYGCRESLVDGIKRATDVMVAGKVAVVCGFGDVGKGCAQALSAL
                     RAQVWVTEIDPICALQASMEGYKVVTLDDVADKADIFVTCTGNYHVITRGHMDRMKDQ
                     AIVCNIGHFDNEIDVASIENLQWEEVKPQVDHVIFPDGKRIILLAKGRLVNLGCATGH
                     PSFVMSASFTNQTIAQIELFTRGEAYKKGQVYVLPKHLDEKVARLHLNKLGVSLTRLR
                     PDQADYIGVPADGPYKPGHYRY"
     gene            15952..16287
                     /locus_tag="VFR20_01795"
     CDS             15952..16287
                     /locus_tag="VFR20_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEU0229280.1"
                     /translation="MEIILVWILNAVALLIVAYILPGINVASFGSALIAALVLGLLNT
                     LVKPVLILLTFPITIVTLGLFLLVINALVFWFAGSILKGFHVGGFWWAVLGAIVYSFI
                     SGLLSRLIH"
     gene            16320..>16494
                     /locus_tag="VFR20_01800"
     CDS             16320..>16494
                     /locus_tag="VFR20_01800"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEU0229281.1"
                     /translation="MTSSDTSLPDSHSGPARRPVSLEFFPPRDIAAQERLTRAAKQLQ
                     AVQPAYVSVTFGAG"
BASE COUNT         2820 a         4842 c         5572 g         3260 t
ORIGIN      
        1 ccgccgatgt cgatatccac cgggccggcg gcgactttgg tgcgctcgct gggcagcatg
       61 gactcgacga atggcacggc agtcgcgacg acgccgactg cgcccagggc cgaggcggcg
      121 ccaaggagga ggcgccggtg atggtcggcg gtgaccattg tgtgggaaga cgacgaaagg
      181 gtttgttcta tttctgtcat ttgagcgcct ttgatctgcg gtgtcccaag gcggcgacgc
      241 gccccggaac ggtggcgatg ccgcacggtc caaaacgggc gcgtgcgcgg ggaaacccct
      301 ttccccgata cttctctctc tgtagagact tcctttggtg tctgaaagtt ccgatgccgg
      361 ttcaaagtga tgccggcaat ctgatggccg tgccgcttat tgtggccgca ttgcaagcca
      421 ttgcggcagc gtttcactgc ggcgaaccaa acggcacatc agccgtcggt atccgcgggc
      481 gcttcggcgg gttggcctcc aggcttgcgc cagagcgcgt cggcgtgtcc cgcagccttg
      541 ttcagcaccc tggccagcac gaagcacaga tccgacagcc gattcagata ctggcgcagc
      601 ggctcgccga cgggttcact gtggtgcagc gccaccacgg cgcgttcggc gcggcggcac
      661 gtggtgcgtg ccagatgggc cagcgccgcg ccgcgcgtgc cgcctggcag aatgaattcc
      721 gtcagcggcg caagcccttc attgtggcgc cgtatggcct cgtccagcca ggcgacgcgt
      781 tcgggcgaca ctgccgcgtg gccaggccag cagagctcgg cgcctagatc gaacagatcg
      841 ttctggatgt cgcccagcat gtcgtccagg ccatcaggaa gtgattctgt gcgccacaac
      901 ccgatgacgc tgttgagttc gtccacgtcg cccagggcca cgatgcgcgg ggcatccttg
      961 tccaggcggc tgccatcggc caggccggtg acgccggcgt caccggtgcg ggtggtgatg
     1021 atggaaagcc gtatcgacat ggtctggttc cgtgaagacg cgcgcgcggc gcctgttgat
     1081 cgagggtggg agtcgtgtgg tttggcgtga ggcccgttgc aaccggtatc agtcgggcat
     1141 ctcggccagc gcccgcaggg cgtcgcccgt gacacgggcg acacgccagt cgtgcaaaat
     1201 cgtggcgcca tgacgcacgt agaaatcgat ggcgttctgg ttccagtcca gcaccgtcca
     1261 ttcgaagcgg ccgcaatcca gtgcgatggc cagccgggcc aggtgcttga gagccagtcc
     1321 acccagtccc cggccgcgat gcgcgggctg aacgtagatg tcctccagat acagtccgcg
     1381 tttgtccagg aagctggagt agttgtggaa ccacatgatg taggccacag gtacggcggg
     1441 gtcgtccagc gtgtgggcga tcaggccatt ggccgcgggt tgcgggccga agaagctgcg
     1501 atgcagactg gcttccgtgg cgtggaaaat atccgtcagt ttctcgaatt cggccatctc
     1561 gcgcatcagg tccaacacct gggggatgtc tgcttcacag atgggccgca actggtaatg
     1621 gctgtttacg tcggacaact tctttcctgg tgatttgggg gtatgcttga gggaaccata
     1681 tcgatgcggt gagcatcaaa ccaccgtatc gaagccgtat tcggttggcg cggtggcttg
     1741 tccagagaac ttctcattat aggctggcac ccgcaccgat acggcgcgcg gcagccctct
     1801 cggggtggcc gttgttgtag acaggagtac gcttcatgac cactacccat cgatggcagc
     1861 aggctttgac ggcggcattg gccgtcacat cggtgtattc gacagccgcc ttggcgcagc
     1921 aggcaaccgc ctcgggccag gtgcgccgcg tggatgcggc cgcgggaaaa atcgccatca
     1981 agcatggcgc catcgccgat cttggcctgc cggccatgac cctggtgtac cacgccgctc
     2041 cgtcattgct cgccggcatc aagccgggcg accaggtcac cttcacagcc aagcgtgaaa
     2101 acaatcagta tgtgatcgtc agcatcaaga aatagggcgt tgccagaacc cgtggcgacc
     2161 ggcgtcgaag ccgttcgcca cgagaggtct gcacggagag cgtgtttggc gacgccctac
     2221 agcacgtacc gcgcgagatc ctggttggcg gcggcttcct cgagtttatc gttgacgtag
     2281 gcggcgtcta tggtgatggt cttcgcgcca tgcgacgccg cgttgtacga caggtcttcc
     2341 agcagccgct ccatcaccgt gtagagtcgg cgcgcaccga tgttctcggt gcgctcgttg
     2401 acgtcgaacg cgatttccgc aatacgctgt atgccgtctt cctggaagtc cagggtgact
     2461 tcctccgttg ccagcaacgc gcagtactgt ttggtcagcg acgaatcggt tttgctgagg
     2521 atgctcacga agtcatccgc cgacagggag tcgagttcga cccggatggg gaagcggccc
     2581 tgcagttcgg gaatgagatc cgacggccgc gacaaatgga acgcccccga ggcgatgaac
     2641 aggatgtggt cggtgtgcac catgccgtac ttggtgttga ccgtggtgcc ctcgaccagc
     2701 ggcagcagat cgcgctggac accttgccgc gacacatcgc cgcccgaatg ctcctggcgc
     2761 gaggcgatct tgtcgatttc atccaggaaa acgatgccgt tctgctcggc gttggcgatg
     2821 gccgtcgtgc gcaggtcttc ttcgttgatg cggcgtgacg cctcttcttc cgtcagcagc
     2881 cggaaggctt ccttgacggt gatcttgcgg tgctttttct tgtcgcgccc gatgccggcg
     2941 aacatgcctt tgagctgttc ggtcatttcc tccatgccgg ggggcgccat gatctccatc
     3001 tgcgccgcgg gctgggcgac ttcgatctcg atttccatac tgtcgagctt gccctcgcgc
     3061 aggcgcttgc ggaacgtctg gcgcgccgcg ttctcttcgc gctggggttc gccctgggcg
     3121 ttgcgcggcg gtgataccag cacgtccaga atgcggtctt cggcggcgtc ctcggcctgg
     3181 gtgcgtacac gccgggtctc gacttcacgc gtttgtttga tggaaatgtc gaccaggtcg
     3241 cgcacgatgg tgtccacatc gcgccccaca tagcccactt cggtgaactt ggtggcctcg
     3301 atcttgatga acggcgcgtt cgccaacttg gccagacgcc gggcgatttc ggtcttgccc
     3361 acgccggtgg gcccgatcat gaggatgttc ttgggcacga tctcgttgcg caagggttcg
     3421 ggcacctgtt ggcgtcgcca ccggttgcgc agggcgatgg ccaccgaacg cttggcgcga
     3481 tcctgcccga tgatgttctt gtcgagttcg gagacgattt ccccgggtgt catggtagag
     3541 gctgacatga tgattgggcg gtccaggcgt attgctacag ggtttcgacg atgtggttct
     3601 ggttcgtgta gatgcagaga tcgccggcga tctccagtga cttcttcacg atgtcggctg
     3661 gtggcaggtc ggtgttgtcc agcagtgcgc gcgccgccga ttgcgcatag gcgccgccag
     3721 agccaatggc cgccaggccg tgctcgggct cgagcacgtc accggtgccg gtcaggacca
     3781 gggtgtgttc gcgatcggcc acaataagca tggcttcaag gcgccgcagg acgcggtcgg
     3841 tgcgccagtc acgtgtgagc tccaccgcgg cgcgcatcag gtggccctga tgtttctcca
     3901 gcttggcctc gaagcgttcc tgcagggtga aggcgtcggc cgtggcgccg gcgaaaccgg
     3961 caaggacttt atcgtggtac aggcggcgga ttttccgggc cgtacccttg atgacgatat
     4021 tgcccagggt gacctgaccg tcgcccccca gggcgacctg gtcgccgcgg cggacgcaga
     4081 tgatggtggt ggcgtgaaat tgttccatgg atttcttcct ttgaaccatt cagatggggg
     4141 cggattctga aagttcaagg catggagcgc gccgccggat cagtccccgt agagtttttg
     4201 ccgcatttca cgccgttcct gggcttccag ggacaaggtg gcggtggggc gggccagcag
     4261 gcgcgcaatg cctatgggtt ctccggtttc ttcgcaccag ccgtattccc ctgagtcgat
     4321 gcgggtgatg gactgctgca ctttcttgag cagcttgcgc tcgcggtcgc gcgtgcgcag
     4381 ttccagggcg tgttcctctt cgatcgtggc ccgatcggcg gggtcgggca cgaactgggt
     4441 ctcgcgcagg ttctcggtgg tggcccccgc gttgttcagg atgctttgct ccaactgctt
     4501 gagacggtcc cggaagaacg caagctgggc ttcgttcata tagtcggatt cgggcattgc
     4561 cagaatttct ttttctgtca gcaactggct gtgaacttta gtggctgcgg atttgcctgc
     4621 catgatgtac ccctttattg tgtatcgtgc tgcttgtccc tgcgtgtgtc ctgaatgtgg
     4681 gtggccgaag atggccgtcg ccttgtagtc aggccaggca ttgctccaga cccctcgtga
     4741 aaatttcctt cgggagttta cgaccgatga aaaccatccg ggtattgggt ttttcacttg
     4801 atagccacgc cttgcccggt tccgcgccca tcatcatgtg caccccctgg aacagcatgc
     4861 ggcggttgat acccttgagg aacaggatac ccttgtagcg cagcagatcg gggccgtaga
     4921 cctggacgat gccgcccagg aattcttcca ggcgctcggg gtcgaacggc cgatcagagc
     4981 ggaacaggaa agcgccgatg ctgtcgtcgt ggtgggcgtg atgatggtca tggtggccgt
     5041 ggccatcgtg gtgatcgtga tcgtcgtcgc cgtcatggtc atggtcatgg tcgtggtgat
     5101 catgatcgtg gcgggcgtcg gggtgctcat cggccaggaa ttccgggtcg atgtcgagaa
     5161 tggtgttgag gttgaaaccg ctgatgtcca gcagcgcatg gaggtcagtg tcgccgaagt
     5221 tgaccggcgt gatcgaggcg cgcgggttga tgcgcaccag ccgagcccgc agcgcctggt
     5281 agtcggcgtc gttgaccagg tctttcttgg aaaccagaag cttgtcggcg aacccgacct
     5341 gcttttgtga ttcttcctgc ttgtccaggg tctccatgcc atgcttggcg tcgacgatgg
     5401 tgatgacggc gtcgagccga tagtagtcgg cgatttcgtc gtccatgaag aacgtctggc
     5461 aaacggggcc agggttggcc atgccggtgg tttcgatgat gacgcgctcg aagatgaggg
     5521 tgcctgcttc gcgccgttcg ctcatgtcgc gcaacgtgcg catcaggtcg ccccggatgg
     5581 tgcagcagac gcagccgttg ctgagctcga tgatctgctc gtcgctgtcc tggaccagca
     5641 ggtcgttgtc gatgctttcg ggtccgaact cgttttcgat gacggcgatg cgctggccgt
     5701 ggtattcgga aagaatgcgc ttgagcagcg tggtcttgcc cgcgcccaga aagccggtca
     5761 gcaccgtgac cggcaccatt ttattcttgg atactgcgtt cattcagagc ctcgcctggc
     5821 gcggcgacga aacagatgtc gcatcgtttc caggtgccgc gggcacgcga agcccgcatt
     5881 cctgggttac gtatttgaca tggcgacagt catttcattc gacaccgcgc ataaaattca
     5941 acgtgcgatt acatcacagc ccgccattta gggcaaacac gcgtcaaacg ggctgttcgg
     6001 cgcgcgcgac actgcgattc gtcaatgaat atagactaat tacccctgaa tggctaatgc
     6061 ggatgggggt ggtcgtgcgc cgccgcgccg cgttccagac attgccggca cagcgcgcgc
     6121 aactctgttt cgtggcccgc cagaacgaat ccggtgccgg ccacgcgttc ggccagctca
     6181 cggctgagga ttgggtcgct gagctcggcc acggcgccgc aatgcgtgca cacgataagc
     6241 aggtcatggg gctcgcccgc cgcgtccagg caggcggccc aggcgttgac cgcatccaga
     6301 cgatggacca gcccctcctc gaccaagaag tccagggcgc ggtatacggt gggaggcacc
     6361 gccccgggct gctccgcgcg aatttcttcg agcaattcgt aggccttcag actgcggcga
     6421 cgccgcaaca gaatctccag caccttgcgc cggatgggcg tgaggcgttt gccgcgttgc
     6481 tcgcaaaggg cttccgccgt gccgagcttg tcggcgagct tttgggacga ggcggtgtga
     6541 tgtccaggca tggcggatag ggcaagggaa ggggaaaaca ggacactagg catggccgac
     6601 aagagggatt attgtgcctg ccagccccca ataatgttaa tgttataaca taacattaac
     6661 tcagtcgcgc ttgcggcggt tggcgcgcgg gtgcgccttg tcgtagacgc tggccagatg
     6721 ctggaagtcg agccgcgtat agatttgcgt ggtcgagata ttggcgtgcc ccagcatttc
     6781 ctggacggcg cgaaggtctt gcgccgattg cagcatgtgg ctggcgaagc tgtggcgcag
     6841 cccatgcggg tggacgtgca ccggcaggcc ggcgcgcacg gccagttgat ccagttgttt
     6901 ctgcacgacg cgcgcggcga tgcggcgacc gcgcatgccc aggaacaggg cggcgccact
     6961 gtccgggtct cccgccaccg gcaattggtc gcgccgggcc agccattgcc gtgtggcctc
     7021 gacggccttg gaccccagca gcacactgcg tgtcttgccg cctttcccac gcacggtggc
     7081 ctcgccttcg tcgagctgga tccagctatg ggattcgtag ccggcctccc gcacgtagcg
     7141 ccagtccagg ccgaccagtt ccgccaggcg caacccgctg gaatacagga tctcgaacat
     7201 agcctggtcg cggcattcag cgatggaatc cgaatgaggt tggccggggc gatcgagcag
     7261 cacctggact tgctccaccg acaaggcctt gggcaggctg cggggtatct tgggtgcccg
     7321 taccccggac gccggattgc tggccatccc ggcgccgggc gcccaccact ggaagaagcc
     7381 gcgccaggct gcgagcgcgc gcgccaggct gcgcggcttg ccgccttgcg cgtgcaagcg
     7441 ggccaaggcc tggcggatat ggctttccgt gtagcgttcc agcgccgtgt caggatgaca
     7501 ggcgacgagg tggagcaaat cgtggcggta gccgtccact gtgtggacgg aatagcgtcg
     7561 ctgggtcagc agatgggcca gccattgctc catgggagcg ggcagtgcgt gcacagaggg
     7621 catgtcgtcg gctgtatgtc ggttctgtgt gcagtgtagt tcagtcggtg tgccatgacg
     7681 ttctcgcgtc tgtgcctgtg tggcgaatcc tcgttgcttg aagtcgcggt tcaaagagag
     7741 gtggcggcga aaaaattcag acgggctgat atggggcggc cttggtccga aatcatcctc
     7801 ttctttcccg gcgggcaact gatcggtcgg cacccgcgca ttaaaatcag ccatttcacc
     7861 ctgcccaccc cgtttcttgg agcccgcatg tcccggttcg ccccttcgga tcttccacta
     7921 ctgtctcggg tcgacgtcat cctgacggat gtcgacgaca ccttgacgca caagggccgg
     7981 ttgtccgcgc gtacgctgga tgcgatggcg cgtctggcgc aggcgggcgt tcgggtggtg
     8041 cccgtgacgg gtggctgcgc gggctggtgc gatcacatcg tgcgcgcctg gccggtcgct
     8101 gcggtcatcg gggaaagtgg ggcgttccgg ttcagcctgc ggcagggtgg cgcgttggaa
     8161 cgttattttg tgcggccgct ggatgaactg cgcgccgatc agcggcgcct gctcgaggtc
     8221 gcccggcgcg cgctggaagc tgtgccggcg gcgcgcatgg cggctgacca ggactatcgc
     8281 ctagtggacg tggcggtgga ccacgcacag gatatcgcgg cgctgccacc tgaaagcgtg
     8341 cagaccatca tcgatgtttt ccagaatgcg ggcgccaggg cgcgcgccag ttccatccat
     8401 gtgaacgcgt ggttcggtga tcacgacaaa gccagcatgg cgagccatgt cctggcggaa
     8461 gatttcggca tatcttcgaa agaagaacgg gccgggcgcg tcctgttcat tggcgatgcg
     8521 ccgaatgatg aaaccctgtt cggcgcgagc acgctgagtt tcggcgtggc caatatcgcg
     8581 ccgcatctcg acgccctggt ccataagccc cggtgggtgt gccagacggc ctatggcgac
     8641 ggattcgcgg aaatggccgg ccacgttctg gccgcccgtg cctgaaagcc catgcgtccg
     8701 gtttgagtgc gcgaaccaat caaaaggcac agtcagatgg gctacgtcgt gcatccaacc
     8761 ccgattcaca gcgcctgctt tccgctcgac ttattcaccg aatttccaat tcaggacatt
     8821 aagggagggc cttcaggcgc agcaggctgg cgctggccag cccgttgatg atttgcagga
     8881 aatcggtgga catgtcgggc gtgaatcggt ccgggtcatc cgagcccagc accagcaggc
     8941 ccagcgtgtc gtgcgaggcg tcggggcgca gggccacgat ggccagcgag gcggggacgg
     9001 aacccagcca agccgcggcc tcctgcccgt ccagcggacc gcaatacggc cgggtcagtg
     9061 cctgggcgta ttgcctggcc gattcggtga cgtcctgggt gaactcgctg tcgaccaacc
     9121 ccggcaggtt ccacaggcgc agtgcgatgg tcggcagatc gaacaggtcg gacaatccac
     9181 gcaccatgtg cgcgggcagc actgtggcgt cgggttcggc cagcatgtgg cggcaccagg
     9241 tattgagcgc agtgctgatc ttctcgttgc tggtggcgtt gtgcatgagc ccggccagtt
     9301 tccattccag atccttggcg cgggtgcgca gagtcagaat ctggcgttca ccgagagata
     9361 tggcgcgtgt ctcatgggga tggggcacgc gcagattggc gaacagatcg gcgtggtcct
     9421 ggaagaattc ggggttggcc agcaggaagt cggcgatatc ctgggcggtg aaactgacgt
     9481 cgctcatggc tggggcgtgt ccttgggttg cgttgcgaga agggcgtcca cgtcgacctg
     9541 gcccgaaaag accgagaccg ccgggccgga catgacgacg cgctcgccat tccatgcgac
     9601 gctcaggtcg ccgccccggg tatgcacccg caccgggctg tcaagcaggc cgcggcgtat
     9661 gcccgcggcc acggcggcgc aggcgccggt gccacaggcc agggtttcgc ccgcgccgcg
     9721 ttcgtagacg cgcaatgcaa tggtgtgccg gtcgagcact tgcatgaatc cggcgttgac
     9781 gcgattggcg aaacgagggt gcgattcgat cagtggcccc acgatcgcga ccggcgcggc
     9841 ctggacgtcg tcaacgactt gcacggcgtg cggattcgaa atggccacca aggaaacatc
     9901 gacactgccg tggccatcga gaggaagtgc ccacagtgtg tcctggccct cggcccgcga
     9961 cgccaggccg cttgtgtcga aggcaacgtc gtcggggttg aaactggtgt ggcccatctc
    10021 gacatcgacg tgacccagcg tgtcttgatt gagcgtgatc aggccggtgt ggatctcggc
    10081 gcgcaacggg ttgcggcccg agaggccctg gtcatggacg aagcgcacga aacaacgggc
    10141 gccgttgccg cagtgttcga cttcaccgcc atcggcgttg aagatacggt agcggaaatc
    10201 ggcgtcggga tgcgaggggg actcgaccag caatatctga tcggcgccga tgccgaagtg
    10261 gcggtccgcc agcgcgcgtg cgcgttccgg cgtcatgtcg atattctggc gcacgccatc
    10321 gagcacgaca aagtcgttgc cggcgccctg catcttggtg aaattccagt tcatgtcgag
    10381 attatcgccc aatctcaata gaatttcggc tcaccttcga tggggcgtgt cttgaatcgc
    10441 cgatggaccc agtaatactg ggctggatcg gaccgcaccc aggattcgat cacgcggttc
    10501 agcctggcgg tcgccacttc caggccatcg tcacctggga aggcgtcgag aggctccagg
    10561 acgtctacat ggtaacggcc ggtgcccagg tccaggcggc tgacgatggg caccacggcc
    10621 gccttccacg cgccggcgat ctgtgccgtg gtcgtgagcg tggccgccgg cacgccgaag
    10681 aatggcggga agatcgcgcc atcacggccg aagtccatgt ctggcaggta atagacggga
    10741 atgccgcgcc gcaggtggcg gatgagtccg cgtacgccgt cctggcggct gatcaggtgt
    10801 acctggttga aacgcccgcg cccctcgcgc acgaggcggt cgacgtcggg gtcgctttgc
    10861 ggcgtataaa gcgtggcgga ttccttcagg aacaaggtca gtcgcgtggc ggcggcatcc
    10921 aggcctatga agtggggcgc cagcaggatg atgggcgttt cgtcccgcaa caggttttcc
    10981 aggtgctcca gtccggagat ccgcaccgtg tccaggatgg cctggcgcgg cccgaaccag
    11041 cacaggcccc ggtctatgac tgattgcccc agcagtcgga agtggcgatg tatcagagca
    11101 tcgatgtcgg atgcgggaag atggggaaag cagagtttca ggttgatgcg caccacccgg
    11161 acgcgcgagc gcagcaggcg cggcgcgagc cagcccagca ggcgccccca gcgcaggcgc
    11221 gctggcgtgg cgcagtgtcc gaaatacgag aacacgccac gtaacacagg gagtttgctg
    11281 aacgtcatgt cgaaggcgga ggcggcggcg cgcctttggg ttgcttgtag cggttgtaac
    11341 cccagagata ttggtcgggc atgcgccgta tcagcgcttc catggtctgg ttcacgagcg
    11401 cggcttggtg atcagggtcg gcgggcagcg gctcgggcag gcgtacgacg tgtatgcgcc
    11461 acccacgtcc gtgcggcaag cgttcgccgg cggcggcgat gacgggcacg tgcgtctggg
    11521 cggcgagttt tcccggcagg gtcatggtgt aggcgggtcg accaaagaat ggcacccaga
    11581 ccccctcgcc actgctcggg gcctggtctg gcaacaggcc gaccacgccg ccgcgccgca
    11641 gggcgcgcac gaattcgcga acccctttca tgttggccgg caccgcgttc agtgtggcgc
    11701 tgttgcgccc ctgttccatg atgggcctga gcatgttttt gcgcggcggg cggaacatga
    11761 cggtcatggg gccgtggacg gcggccatgt aacgggcggc gatttcgaag ccgcccaggt
    11821 ggggtgtcag gcacaggacg ccgcgtccct gcgcgacggc atccgccacg atcgccatgt
    11881 cgtcatcgcc ggcgatgatc ttgtccaggc actgttccgt gcgcagccac actttgggca
    11941 attccatgat catggcgccg gtctgggcgg cggcccgccg tgcgaacacg gcatcggaat
    12001 agccggcctg ccgggcgttg tcacgcaggc ggcggcggta tttgccgggc aaggcgtaga
    12061 cgatccgccc gccgatgcgc cccacgccat gcaggagcgg cagcggcacg caggccaaca
    12121 ggcggaacag aaacagcagc atgggcgggc atattcctcg tggaaataac gtaatcaagt
    12181 gcgccgggct attttcgctt aaaataccag gtatcgctga gttaaccgac aacttgcgga
    12241 gcgatgacgg gatggcccgc cggccctact gtcaaaaact gctaaagcgt cgcgccttga
    12301 ttcgaattgc cgtattggtc ttgggtgcaa cgcgttttgt tcaaccttaa ggctgggtga
    12361 ccagcggcaa tcaaggacga cttctcgtgg ccaacaacga ttttctcttc acttccgaat
    12421 ccgtctcgga gggccatccc gacaaggtgg ccgatcaaat ctctgacgcc attctcgacg
    12481 ccattttcac gcaagacccg aattcccgcg tcgcggccga aacgctgtgc aacaccgggc
    12541 tggtggtgct ggcgggcgaa atcaccacga cggcgaacgt ggattatatc caggtcgcgc
    12601 gcgacaccat caagcgcatc gggtacgaca acaccgacta cggtatcgac tacaagggtt
    12661 gcgcggtgct ggtcgcgtac gacaagcaat cgcccgacat cgcccagggg gtcgaccgca
    12721 ccgccgaaga aatcctgaac cagggcgcgg gcgaccaggg cctgatgttc ggctatgcgt
    12781 gcgacgaaac ccccgacctg atgccggcgc ccatctggta cgcgcaccgc ctggtgcagc
    12841 gccaaagcga actgcgcaag gacggccgcc tgccgtggtt gcgccccgat gccaagtccc
    12901 aggtcacgtt ccgttatgtc aacggcaagc cggccgaggt cgatacggtg gtgttgtcca
    12961 cgcagcatgc ggaagaaatt tcgcaaaaag acatccacga ggcggtgatc gaagagatca
    13021 tcaagccgtg cttccccaat gggctggtta cgcccaagac gaagtatctg gtgaatccca
    13081 ccgggaagtt cgtcatcggc ggcccgcagg gcgactgcgg gctgacgggg cgcaagatca
    13141 ttgtcgatac gtacggcggc gcgtgcccgc acggtggcgg ggcgttctcg ggcaaggatc
    13201 cgtcgaaggt ggatcgttcg gcggcatatg cggcacgcta tgtggcgaag aacatcgtcg
    13261 cggcgggtct ggcgcgtcag tgccaggtgc aggtcagtta tgccatcggg gtggccgaac
    13321 ccatcaacat cacggtgtac acggagggca cgggggtgat ccccgatgat cagatcgcgc
    13381 gtctggtgcg cgagcatttc gatctgcgtc cgaaggggat catcaatatg ctggatctgt
    13441 tgcgccccat ctatgcgaag acggcggcat atgggcactt cggtcggtcg gagcctgagt
    13501 tcagctggga ggcgaccgac aaggttcagg tgctgaagaa ggcggcctga tatcggtgtg
    13561 tgtcggcggg ggcatgcggg cggtgtcgtt tgcatgcccc cgctgtcgtt ttgagcgtgg
    13621 gggatgtggg tcatggcgga gcgcggggag tcggtcgatg gggcgccggg cgcagggagt
    13681 gtcgggggtg ccggcgaccg cgatggcagc cgcagtctgg agcggaccga ctctccgtgt
    13741 gtggcgctgt gttcgacgct ttacgatgat gtgtgccggg ggtgtgggcg cacggtcatg
    13801 gaggtggcga actgggtgtt catgagcgac gatgaaaaac ggcgcgtctg ggagcgcatc
    13861 cgggcggagg ggtatcctcg gaggcggtag ggtggcttca tcttccttga tgccgcaggt
    13921 tgcgttcttt agccggtgcg ggcgggtggc ctgtgccggg cctccgtcgc tgcggtccgg
    13981 ctgtgccgga cttcccggcc agacggttca gcgcggggcg gtcgcgaaac tcgcggggcg
    14041 atcagcccac tgggctgatc gcaggcgtcc cgctcaaaca gtcgctccct tgcctccccg
    14101 cactgagccg tctggccggc ttgcgacaaa gtcggcccgg cacaggccac ccgccctcac
    14161 ctctttgtgg gggctggcga aggctttgtg cgggctggcg aagtggagtt cggcgggcgg
    14221 tcagcgggtg tgtgagaggc gttcttgctg gctcacaggt ctgatagtta cgctacaatt
    14281 gtggttttcc tgaggggcgc tgcgacgggc gtgcgagggc tgtttggccc tgctttgaac
    14341 cccgctaggc tcaggactgt ctcttttgaa cggcgctcat catttcccgc ggggcgggtg
    14401 gcgatgggca ggttcgtgtt catgcgtccg cattgcgggt cttggagaaa gacccatgaa
    14461 cacagttacc gataccgttt tttccgacta caagattgcc gacatcggcc tggctgattt
    14521 cgggcggcgc gagatcgcga tcgccgaaac cgaaatgccg gggctgatgg ccacgcgcga
    14581 ggaatatgcc tcggcgcagc cgctgaaggg tgcgcgcatc gccggcagtc tgcacatgac
    14641 catccagacc gcggtgttga tcgaaacgct ggtggcgctg ggcgccgagg tgcgctgggc
    14701 atcgtgcaat attttctcga cccaggacca cgcggcggcg gcgatcgcgg ccggtggcaa
    14761 tccggtgttc gcggtcaagg gcgagaccct ggttgattac tggcggtaca cgcacaagat
    14821 tttcgaatgg cccgaaggct catcggccaa catgatcctg gatgacggcg gcgacgcgac
    14881 gctgttgctg catctgggca cgaaggctga atcgaatccg gccgtgctcg acaaaccgaa
    14941 cggcgaggaa gagcgtgtgt tgttcgcggc gatccgcgag cgtctggcgg tggattcgac
    15001 atggtattcc cgccgtctgg cgcagattcg cggggtcacg gaagagacca ccaccggggt
    15061 gcatcgtctg taccagatgt cgcagaaggg tgaactggcg gtgccggcga tcaacgtgaa
    15121 cgattcagtc acgaagtcga agttcgacaa tctgtatggt tgccgtgagt cgctggtcga
    15181 cggcatcaag cgcgcgacgg atgtcatggt ggcgggcaag gtcgcggtgg tctgcgggtt
    15241 cggtgatgtg ggcaagggct gcgcgcaggc gttgtcggcg ttgcgggccc aggtgtgggt
    15301 gacggaaatc gatcccatct gcgcgttgca ggcgtccatg gaaggctaca aggtggtgac
    15361 gctcgacgac gtggccgaca aggccgacat tttcgtcacg tgcacgggca attatcatgt
    15421 gattacgcgc gggcacatgg atcgcatgaa agaccaggcc atcgtttgca acatcgggca
    15481 cttcgacaac gaaatcgatg tggcctcgat cgagaacctg cagtgggaag aagtgaagcc
    15541 gcaggttgat catgtcattt tccccgatgg caagcgtatc atcctgctgg ccaaggggcg
    15601 tctggtgaac ctgggttgcg cgaccgggca cccgtcgttt gtgatgtcgg cgtcgttcac
    15661 caaccagacc atcgcccaga tcgagctctt cacgcgcggc gaggcttaca agaaagggca
    15721 ggtatatgtg ctgcccaagc atctggacga aaaagtggcg cgcctgcact tgaacaagct
    15781 gggtgtgtcc ctgacgcgtc tgcgtcctga ccaggcggac tacatcggtg tgccggccga
    15841 tggcccgtac aaaccgggtc attaccgtta ttgatgcatc actgccgact catcggccgc
    15901 ggcccggcat gagcgccgcg gccgacgatt caccacaggg agggtcgact catggaaatc
    15961 attcttgtct ggatactgaa cgcggtcgcg ctgctgatcg tggcgtacat actgccgggg
    16021 atcaacgtgg cgtccttcgg gtcggccctg attgccgcgc tcgtgctggg cctgctcaac
    16081 acgctggtca agccggtgct catcctgctt acatttccca ttaccattgt caccctgggc
    16141 ctgttcctgc tggtcatcaa tgccctggtg ttctggttcg cgggctcgat cctgaaaggc
    16201 tttcatgtgg gcggcttctg gtgggccgtt ctgggcgcca ttgtctatag ctttatttcc
    16261 gggctgttgt cgcggctcat tcactagccg gtgcgtcccc actcattttt tgatgatcaa
    16321 tgacttcctc agacacgtcc ctgcctgatt cacacagcgg cccggcgcgg cgccccgtaa
    16381 gcctggaatt cttcccgccg cgcgacatcg cggcgcagga aaggcttacg cgcgccgcca
    16441 agcagttgca ggccgtgcag cctgcttatg tcagcgtcac gttcggggcg ggcg
//
LOCUS       DASTNK010000025        14243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1125724, whole genome shotgun sequence.
ACCESSION   DASTNK010000025 DASTNK010000000
VERSION     DASTNK010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14243
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125724"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1321
                     /gene="hemA"
                     /locus_tag="VFR20_01805"
     CDS             47..1321
                     /gene="hemA"
                     /locus_tag="VFR20_01805"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEU0229282.1"
                     /translation="MSVDVLTFGLNHVSAPVAVRERVSLPLDLLKPALDGLRSAFGSS
                     VKEAAILSTCNRTEIYCAAEPSVSEQLPAWMADFNRMEAGSLQPHLYRYERNEAVRHA
                     FRVASGLDSMVLGEPQILGQLKDAVRAAEEAGSLGTLLHQLFQRTFSVAKEVRSQTAI
                     GAHSVSMAAAAVRLAERVFGDLAQASVLFIGAGEMIELCATHFAALNPRRMSVANRTL
                     ERADVLAGRFMAKTMKLSELPDKMADFDVIVSCTASSLPILGLGMVERATRSRRHRPM
                     VMIDLAVPRDIEPEVGRLADVYLYSVDDLGRMVQSGTDARRAAVVQAEAIIETRVQNF
                     MHWLQSREIVPAIIALQRSADQVREAELERARRMLARGEDVDQVLEQLAHALTQKYMH
                     GPLAALNRSEENERAQLLDLLPRLLPDANRRR"
     gene            1416..2495
                     /gene="prfA"
                     /locus_tag="VFR20_01810"
     CDS             1416..2495
                     /gene="prfA"
                     /locus_tag="VFR20_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU0229283.1"
                     /translation="MKSSVRNRLEQLSHRLIEIDALLAEPDIAGDMDRFRKLSRERAE
                     LEPVVEAFRAYTSAEADVQVATEMLADPDMKAMAEEELKLGRARIEQLNADLRVLLLP
                     RDPDDGRSVFLEIRAGTGGDESALFSGDLLRMYTRYAEQRGWRVEFMSESPSELGGYK
                     EVIARIDGDGVYGRLKFESGAHRVQRVPETETQGRVHTSACTVAILPEADEQADIVIN
                     PGDLRIDTFRASGAGGQHINKTDSAVRITHLPTGLVVECQDDRSQHRNKDKAMQVLAA
                     RLKDRQQQAQHSKEAAQRKSLVGSGDRSERIRTYNYPQGRVTDHRINLTLYKLQQIME
                     GNLDELTGALIAEHQAEQLAAMGQE"
     gene            2526..3392
                     /gene="prmC"
                     /locus_tag="VFR20_01815"
     CDS             2526..3392
                     /gene="prmC"
                     /locus_tag="VFR20_01815"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU0229284.1"
                     /translation="MCIRDLLRAAELPSLEARMLLQHVLGVPRVWLITHDTDAVPAGH
                     TERFRMLCAQRREGQPMAYLVGLREFMGHEFRVVPGVLIPRPDTELLVETAIEELAGH
                     VAPRVLDLGTGSGAIAVSVALACPHARVVATDSSETALDLARNNAERLQAGVTFHQGD
                     WYGALPGAGHGAGGVFDLILSNPPYIACGDPHLAEGDLRFEPRRALTDEADGLGALRT
                     IIAGAPHRLRQGGALWVEHGWDQAGAVRDLLRASGFRGVASRRDLAGIERISGGVVGG
                     PGGAPADSSATP"
     gene            3475..3801
                     /gene="grxD"
                     /locus_tag="VFR20_01820"
     CDS             3475..3801
                     /gene="grxD"
                     /locus_tag="VFR20_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEU0229285.1"
                     /translation="MSDVQEFIRETVTSNPVVLFMKGTAQMPMCGFSGRAIQILQASG
                     VSKLVTVNVLDDDEVRQGIKDYSNWPTIPQLYVSGQFIGGSDILSEMYDNGELATVLK
                     EAGAVA"
     gene            3812..4396
                     /locus_tag="VFR20_01825"
     CDS             3812..4396
                     /locus_tag="VFR20_01825"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009464105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEU0229286.1"
                     /translation="MATERRLVVGITGATGAIYAVRMLKALREATDVRTHLVVSPAGV
                     LNIKYELGLGRGDVHALADHVHGFRDVGASLASGGFDTMGMVVVPCSMRTLAAVAHGL
                     SDNLITRAADVTLKERRRLVMMVRETPLNLAHLRNMTAVTEMGGIVFPPLPAFYHHPA
                     TLDEMVDHTVARVLDLFGIHVPGPRWEGTQTFTD"
     gene            complement(4524..5603)
                     /gene="ttcA"
                     /locus_tag="VFR20_01830"
     CDS             complement(4524..5603)
                     /gene="ttcA"
                     /locus_tag="VFR20_01830"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEU0229287.1"
                     /translation="MPVDALLPNGEGTPSSSRRADPRKRKIDDNKLAKRLVRETGRAI
                     ADFNMIEDGDKVMVCLSGGKDSYSLLDLLLTLRARAPINFDIVAVNLDQKQPGFPEHV
                     LPDYLTALGVPFHIETQDTYSVVTRVIPEGKTMCSLCSRLRRGILYRVASELGATKIA
                     LGHHRDDILGTFFLNLFYGGRMKAMPPKLVSDDGRHVVIRPLAYVPEKDLVAYARLKQ
                     FPIIPCNLCGSQENLKRKEVSRMIAEWDRLYPGRSWNVFGSLSRVVTTHLMDKQLCDF
                     AGLKPTGVADAGGDTGFDQEEFPDLPFVAGSRDSDGETRIPDTESGIEPSSAGSRPRR
                     VVYMTDLRQHENDASRVAAEPPAPR"
     gene            complement(5628..5993)
                     /gene="folB"
                     /locus_tag="VFR20_01835"
     CDS             complement(5628..5993)
                     /gene="folB"
                     /locus_tag="VFR20_01835"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEU0229288.1"
                     /translation="MSIRRIFFSQLALDARIGILEHELRATQPLHIDAEFDVDVRHSV
                     LDQDIQTVLDYRLLRGAIVDECTRAHVNLLETLIERVADRLMREFPDVLRLKIRISKP
                     LAFSDCAAVGIEIEQARAA"
     gene            6169..7062
                     /locus_tag="VFR20_01840"
     CDS             6169..7062
                     /locus_tag="VFR20_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0229289.1"
                     /translation="MSVQDQVFDAVIVGAGPAGVSCAVWLARLGFAPALIEASDHIGG
                     LCRANPYVDDWNACLPGMTGDQIADNLAQSLRLAGLEPRLSSFVRGVRAQPRGVSVML
                     QSGDASLSGRFLVLATGVRARPLPGAGDAVPGVLIGPGRHIMAQDFRGLRVAVLGGGD
                     NAFENALYATGHGAAAVRLFARTVRAQRQLVRKVPVAQVVLGDYVVDAGARTVNGEAF
                     DLILVLYGWDPCAEFAQTLDLRRSEHGFIATDQVTTLTSHHLVYAIGEVAQRLHPCVV
                     TAMADGIAAAKAIQARIEADR"
     gene            complement(7178..8968)
                     /locus_tag="VFR20_01845"
     CDS             complement(7178..8968)
                     /locus_tag="VFR20_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU0229290.1"
                     /translation="MQMDKNSILYSLTPFDPAGHLFKVRISVPRPRPRGQTLRLPAWI
                     PGSYLIRDFSRQIQSIQARSGSGHITITKTDSHTWRCAPCQGPLHVEYTVYAWDLSVR
                     GAHLDESHAFFNGASVFLSADGFERQPCLLELCPPPHTRRWRVYTSLPEATGHRDAAR
                     RHGFGLYAAPGYDALIDHPVEIGTPQVARFRAHGAEHELVFTGAAPRLDLDRIATDAR
                     RICATQIELFEPETRQAPFLDSTNRYVFLTTVTADGYGGLEHRASTALITSRHGLPAQ
                     GRPDTPEGYAVFLGLLSHEYFHTWNVKRIKPAAFAPYDLSRPNHTRLLWVFEGFTSYY
                     DDLMLKRSGVIDEAGYLRLIGKTVTAVRRNSGRHKQTVAESSFDAWTRYYKQDENSPN
                     ALVSYYTKGSLVALGLDLTIRRATQGRRSLDDVMRLLWRRYGRRFFTGPRPGLGVPED
                     AIAGIIHDATGVDVRGFLDRHVEGCDDVPLETLLATQGFALEGKANEGRPSLGIRTRS
                     VNDETRIATADDDGAAHAAGLSAGDLLTAVDGLRITNQASLDRVLDRHLPGDSVTVHV
                     FRGDELRHFTVTLAAPAPTEYIVTRATQRD"
     gene            8986..9762
                     /locus_tag="VFR20_01850"
     CDS             8986..9762
                     /locus_tag="VFR20_01850"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU0229291.1"
                     /translation="MNAPLIKVETRGRVGLLTIDRPKVLNALSNDVITELYSALRAFD
                     ADDGIGAIVLTGSDKAFAAGADIGAMKDWTFQDVYGQDYLGHDWEALKRIRKPIIAAV
                     SGYALGGGCELAMACDFIIAGENAKFGQPEIKLGVIPGYGGTQRLPRAVGKAKAMDLV
                     LTARFMDVTEAERAGLVSRVVPVERVLDEALEAAAVIASMSLPSVMMAKECINRAYEA
                     PLNEGLLFERRNFHALFATHDQKEGMGAFVDKRKPDFKHR"
     gene            10337..10810
                     /locus_tag="VFR20_01855"
     CDS             10337..10810
                     /locus_tag="VFR20_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEU0229292.1"
                     /translation="MPVDDVSAHGADGGDVAGVGPEGLEFNDADLAALNAGAGRGIVR
                     GLVAQAIMGGLAALVSWGVAGSFAGLSALAGAAAYFVPNALFASRLLLGSAGGRRPGP
                     LAFFVGEALKLGLAVLLLGLAVRLGHAWLVWPAFLLGLLCVLKGYVLLLAVRGLR"
     gene            10883..11764
                     /gene="atpB"
                     /locus_tag="VFR20_01860"
     CDS             10883..11764
                     /gene="atpB"
                     /locus_tag="VFR20_01860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU0229293.1"
                     /translation="MAAPIGVSPQAEYIQHHLIHLNNTGAKQIGVVNFGVVNYDSLFW
                     SILMGIIVVYFLWRATRSVTTGVPGRFQVSVEMLIDWVENEAKGIIANDVSRRFMAPL
                     ALTVFLWIALMNALDLVPVDLLGLIFKDTGLGANHHDLLYYHRILPTADLNVPMGMSL
                     GVLLMLFYYGIKIKGVGHFIKEMFIAPFHGHGVMALCLAPFNFLLNIIEYAAKAVSLG
                     MRLFGNMFAGELIFLLIALLGGAWTGFNGMSISLGIGHVIAGSMWSIFHVLIVLLQAY
                     IFMMLTLAYVGLAHETH"
     gene            11874..12116
                     /gene="atpE"
                     /locus_tag="VFR20_01865"
     CDS             11874..12116
                     /gene="atpE"
                     /locus_tag="VFR20_01865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU0229294.1"
                     /translation="MTNVAFVALACGLIIGLGAVGACIGIGVMGSKYLEASARQPELM
                     NALQTKMFLLAGLIDAAFLIGVGIAMLFAFANPFVG"
     gene            12239..12709
                     /locus_tag="VFR20_01870"
     CDS             12239..12709
                     /locus_tag="VFR20_01870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU0229295.1"
                     /translation="MNINATLFFQMIVFFVLGWFTMKFVWPPMTKAMDERRQKIADGL
                     AAAEKGKADLAQAQARVSLIEASSKSETHARLVDAEKQGALLIDEARREAEAEKARII
                     AQAQQEARQEIQRVREGLRADVALLAVKGAEQILRREVDAKAHAELLDRLKAEL"
     gene            12722..13261
                     /locus_tag="VFR20_01875"
     CDS             12722..13261
                     /locus_tag="VFR20_01875"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU0229296.1"
                     /translation="MAELSTIARPYAEALFAAANEDPAGLASWSALVDELAQVAGLSD
                     VREALTDPRLTNAARIELFVGLIKAPLSQWARNFVELLVNNHRILVLPQIAEQFEVLK
                     NQHEGTALAQITSAFALDDKQVRELIAGLEKKFGLKLKPSVTTDPSLIGGVRVVVGDQ
                     VLDTSVRAQLVRMRDKLAA"
     gene            13297..>14243
                     /gene="atpA"
                     /locus_tag="VFR20_01880"
     CDS             13297..>14243
                     /gene="atpA"
                     /locus_tag="VFR20_01880"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008865155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU0229297.1"
                     /translation="MQLNPSEISELLKSRIEGLGASTDVRTQGTVVSVTDGITRIHGL
                     SDVMQGEMLEFPNNVYGLALNLERDSVGAVILGDYTGVSEGDPVKTTGRILEVPVGPE
                     LRGRVVDALGNPIDGKGPINAKATDVIEKVAPGVIARQSVTQPLQTGLKAIDSMVPIG
                     RGQRELIIGDRQTGKTAVAIDAIISQRGKGVTCVYVAIGQKASTISNVVRKLEEYGAL
                     EYTIVVAAAASDSAAMQYLSAYAGCTMGEYFRDRGEDALIIYDDLTKQAWAYRQVSLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEDYVEKFTKG"
BASE COUNT         2442 a         4236 c         4766 g         2799 t
ORIGIN      
        1 catgcgcgct ttgtggccgc ttcatagtgg tcaatccaca cataaaatgt ccgtcgacgt
       61 cctgactttc ggcctgaatc acgtctcggc accggtcgcg gtgcgcgagc gcgtgtcctt
      121 gccgcttgac ctgctcaagc cagcgcttga cggcctgcgt tcggcgtttg gctcgtcagt
      181 caaggaagcg gccattctgt cgacctgtaa tcgcacagaa atctattgcg cggccgaacc
      241 atcggtgtcc gagcaattgc cagcctggat ggcggacttc aatcgaatgg aagccggcag
      301 cctgcaaccg cacctctacc gctatgaacg caacgaggcc gtgcgccatg cgttccgggt
      361 ggccagcggg ctggattcca tggtgctggg cgagccgcag atcctgggcc agttgaaaga
      421 tgcggtacga gccgctgaag aggccgggtc gctgggcact ttgctgcatc agttgttcca
      481 gcgcacgttc tcggtggcca aggaggttcg ctcccagacc gccattggcg cgcattcagt
      541 gtccatggcg gcggccgcgg tgcggctggc tgaaagggtg ttcggtgacc tggcccaggc
      601 cagcgtgctg ttcatcggcg ccggcgaaat gatcgaactc tgcgccaccc acttcgcggc
      661 gctcaatccg cggcgcatgt cggtggccaa ccgcacgctc gagcgcgccg atgtcttggc
      721 gggccgtttc atggcaaaga ccatgaaatt atccgaactg ccagacaaaa tggcagattt
      781 cgacgtcatc gtatcctgta cggcaagctc cttgcccatt ctcggtctgg ggatggtgga
      841 acgggccacg cgctcgcggc gccaccggcc catggtcatg atcgatctcg ccgtgccgcg
      901 cgacatcgaa cccgaggtcg gccggctggc cgacgtttat ctgtattccg tggacgatct
      961 gggtcgcatg gtgcaaagcg gcaccgacgc gcggcgcgcc gccgttgtgc aggccgaagc
     1021 gatcatcgag acgcgggtgc agaacttcat gcactggctg caaagccgcg aaatcgtgcc
     1081 ggccatcatc gcgttgcaga ggtcggccga ccaggtgcgc gaggccgagc tcgaacgggc
     1141 gcggcgcatg ctggcgcgcg gggaagacgt agaccaggtg ctcgaacaat tggcgcacgc
     1201 cctgacccag aagtacatgc acggcccgct tgccgcgctc aatcgcagtg aagaaaacga
     1261 gcgcgcgcaa ttgctcgatc tgttgccccg cttgctcccc gacgccaatc gtcgtcgtta
     1321 gcggcgctcg ccgcttcgcc ggcttggtcc ggcagctccg tggttccgct gttttccgtt
     1381 gtcctccttt ttcgttttct tccgtttccc cacccatgaa aagttccgtg cgcaatcggc
     1441 tggagcagct gtcccaccgt ttgatagaaa tcgatgccct gttggccgaa cccgatatcg
     1501 cgggcgacat ggatcgattc cgcaagctgt cgcgcgagcg cgccgagctc gaaccggtgg
     1561 tcgaagcgtt ccgcgcctac acgagcgccg aggccgacgt ccaggtggcc accgagatgc
     1621 tggcggatcc cgacatgaag gccatggccg aggaggagct caagctgggc agggcgcgca
     1681 tcgagcagct caatgccgac ctgcgggtgc ttctgctgcc gcgcgatccc gatgatggcc
     1741 gcagcgtttt cctggaaatc cgcgcgggca cgggcggcga tgaaagcgcg ttgttctccg
     1801 gcgacttgct gcgcatgtac acgcgctacg ccgagcagcg cggctggcgg gtcgaattca
     1861 tgtccgagag cccgtccgaa ttgggcggct acaaggaagt gatcgcgcgc atcgatggcg
     1921 acggcgtgta cggtcggctg aagttcgaat cgggcgcgca tcgggtgcag cgtgtaccgg
     1981 aaaccgaaac gcagggccgg gtgcatacgt cggcctgcac ggtggccatc ctgcccgagg
     2041 ccgacgagca ggcggacatc gtgatcaacc cgggcgacct gcgcatcgac acattccgcg
     2101 ccagcggcgc gggcggccag cacatcaaca agacggattc cgccgtgcgc atcacgcacc
     2161 tgcccacagg tctggtggtg gaatgccagg acgaccgctc gcaacatcgc aacaaagaca
     2221 aggccatgca ggtgctggcc gcgcgcctga aggatcgcca gcagcaggcg cagcacagca
     2281 aagaggcggc ccagcgcaag agcctggtgg gttccggtga tcgatccgag cgcatccgca
     2341 cctacaacta cccgcaaggc cgcgtgacag accaccgcat caacctgacg ctgtacaagc
     2401 tgcagcagat catggaaggc aacctggatg agctcaccgg tgcgctgatc gccgaacacc
     2461 aggccgagca attggcggcg atggggcagg aataggcggg ggacgcggtg tcgacgatag
     2521 agccgctgtg catacgcgac ctgttgcggg ccgcggagct gccttcgctt gaggcccgca
     2581 tgctgcttca gcatgtgctg ggtgtgccac gtgtctggct gatcacccac gataccgacg
     2641 cggtgccggc ggggcacacg gaacgcttcc gcatgctctg tgcgcaaaga cgggaaggtc
     2701 agcccatggc ttatctagtg gggctcaggg agttcatggg ccatgaattc cgcgtggtcc
     2761 ccggcgtatt gattccccgc cccgataccg agttgctggt cgagaccgcg atcgaggaac
     2821 tcgcggggca tgtggcgccc agagtgctgg atctgggtac gggcagcggc gccatcgcgg
     2881 tgtcggtggc cctggcgtgt ccgcacgcgc gagtcgtggc gacggatagt agcgagacgg
     2941 ccttggatct ggcgcgaaat aacgccgagc gccttcaggc gggtgtgaca ttccaccagg
     3001 gcgactggta tggcgcgctg ccaggggctg ggcatggcgc gggtggggtc tttgacctga
     3061 tcctgtccaa tccgccttat attgcctgcg gcgacccgca tctggccgag ggggatttgc
     3121 gcttcgagcc caggcgggcg ttgaccgacg aggccgatgg cctcggtgcc ttgaggacga
     3181 tcattgccgg ggcgccgcac cgattgcggc agggtggcgc gctgtgggtg gaacatggct
     3241 gggaccaggc cggcgcggtg cgcgacctgc tgcgcgccag cggtttccgc ggcgtggcga
     3301 gtcggcggga cctggccggt atcgagcgga tttccggggg ggtggtggga ggccccggcg
     3361 gtgcgccagc ggattcttcc gccacgccgt aaaatcaccg tatcgcgggt ggcgcaggca
     3421 ccttatcatg cccgtcggat gcccgatgtt cccttttctg gatgagagat caccatgagc
     3481 gatgtgcaag agtttattcg tgaaaccgtt acctccaacc ccgtcgtcct gttcatgaag
     3541 ggcacggcgc agatgcccat gtgcggtttc tccgggcggg ccatccagat tctgcaggca
     3601 tcgggtgtca gcaaactggt gacggtcaat gtgctggacg acgacgaagt gcgtcagggc
     3661 atcaaagact actccaactg gcccaccatt ccccagttgt atgtgtcggg ccagttcatc
     3721 ggtggctccg acattctgtc cgaaatgtac gacaacggcg aactggccac cgtgctgaag
     3781 gaagccggcg ccgttgcctg agcagccgcg catggcgact gaacgccggc tggttgtcgg
     3841 catcaccggc gcgaccgggg ccatctatgc ggtgcgcatg ttgaaggcgc tgcgcgaggc
     3901 caccgatgtg cgtacgcacc tggtggtgtc gcccgcgggc gtgctcaata tcaagtatga
     3961 actgggcctg gggcggggcg atgtccacgc cctggcggat cacgtccatg gttttcgtga
     4021 cgtgggcgcg agcctcgcca gcggcgggtt cgacaccatg ggcatggtgg tggtgccgtg
     4081 ttccatgcgc actctggcgg ccgtcgcgca cggcctgtcc gacaatctca tcacccgcgc
     4141 ggccgacgtc acgctcaagg aacgccgccg cctggtgatg atggtgcgcg aaaccccgct
     4201 gaatctggcg catctgcgca acatgacggc ggtcactgaa atgggcggca ttgtgtttcc
     4261 gcctctgccg gctttttatc accatcccgc cacgctggac gaaatggtgg accacacagt
     4321 ggcgcgggtg ctggacctgt tcggcatcca cgtgcccggt ccgcgctggg aaggtaccca
     4381 gacgtttacc gattagaagc ggccatgccg gaaatcgccg ctgcggcgtt tggcgttgga
     4441 gccctgtcgt ttcgcggcgc aagggggcgt cgataggcct gcaattgtcc cgctcgcccc
     4501 ggaatccaac gatgtgcccg gtttcagcgt ggagcgggag gctccgccgc cacgcgtgac
     4561 gcgtcgttct cgtgttggcg caggtctgtc atgtagacga cccgtcgcgg tctggatccg
     4621 gcagaggagg gttcgatgcc ggattcagtg tcagggatgc gtgtctcgcc atcgctgtcg
     4681 cggctccccg cgacgaacgg gagatcgggg aattcttctt ggtcgaatcc ggtgtcaccc
     4741 ccggcatcgg ccacacccgt cggtttgagc cccgcgaaat cgcacaattg cttgtccatc
     4801 agatgcgtcg tcacgacacg cgacagggat ccgaacacgt tccatgaccg ccctggatac
     4861 agcctgtccc attccgcgat catgcggctg acttccttgc gcttgaggtt ctcttgcgag
     4921 ccgcacaggt tgcaggggat gatggggaac tgcttgagcc gggcgtaggc caccaagtct
     4981 ttttccggca cgtaggccag ggggcggatg accacgtggc ggccatcgtc ggacacaagt
     5041 tttggtggca tggccttcat gcggccaccg tagaacaggt tgaggaagaa cgtgcccagg
     5101 atgtcgtcgc ggtggtggcc cagggcgatc ttggtggcgc ccaattcgct cgccacccgg
     5161 tacaggatgc cgcgccgcag ccgcgaacac agcgagcaca tggtcttgcc ctcgggaatg
     5221 acccgggtga ccaccgaata agtgtcctgc gtctcgatat ggaatggcac accgagcgcg
     5281 gtgaggtagt cgggcagaac gtgctcgggg aatccaggct gcttctggtc caggttgacc
     5341 gccacgatgt cgaaattgat cggcgcccgc gcgcgcagcg tcaacaacag atccagaagg
     5401 ctatacgaat ccttgccgcc cgacaggcac accatcacct tgtcgccgtc ttctatcatg
     5461 ttgaagtcgg cgatggcgcg gcccgtttcg cgcaccaggc gcttggccag tttgttgtcg
     5521 tcgatcttcc gcttcctggg atcggcgcga cgagaagaag agggcgtgcc ttccccgttg
     5581 ggaagcagtg catcgactgg catggtgtga atgtccggta aaagcgttca ggccgcgcgg
     5641 gcctgttcta tttctatgcc caccgcggcg cagtctgaga aggccagggg tttgctgatg
     5701 cggatcttca ggcgcagaac atcggggaat tcgcgcatca gacggtcggc gacccgctct
     5761 atgagggtct cgagcaggtt cacgtgggcg cgtgtgcatt cgtcgacgat ggcgccgcgc
     5821 agcagccggt agtccagcac ggtctggatg tcctggtcca gcacactgtg ccgcacgtcc
     5881 acgtcgaact ccgcgtcgat gtgcaagggc tgcgtggcac gcaactcgtg ctcgagtatt
     5941 ccaatgcggg catccagcgc gagctgggag aaaaatatgc gtcgaattga catggcgttg
     6001 gcggtgatgg atttgccgac cattgtagtg cgatcgcgat gtggggcgtt ttggccggca
     6061 gtgtgaccga cgcgccgttt ggtggattcg cgcattccaa aattcaggtg catgggccac
     6121 ccggtacaat ttcgggattc agacaggcaa tatggagcgc gtggcggtat gtcggtgcaa
     6181 gaccaggtgt tcgatgcggt gatcgtcggg gcggggccgg caggggtgtc gtgcgccgtg
     6241 tggctggctc gcctgggctt tgctcccgcg ctgatcgagg cgtccgatca tattggcgga
     6301 ctatgtcgcg ctaatcccta tgtggatgac tggaacgcct gtctgccggg catgacgggc
     6361 gaccagatcg ccgacaatct ggcccagagc ctgcggctgg cgggtctcga gcctcgattg
     6421 tcgtcttttg ttcgaggggt acgggcgcag ccacggggtg tttccgtgat gctgcagtcc
     6481 ggtgatgcca gtctgtcagg ccgtttcctg gtgttggcca cgggggttcg cgcccgtccg
     6541 ctgccggggg cgggcgatgc cgtaccgggt gtgctgatcg ggccgggccg ccacatcatg
     6601 gcgcaggatt ttcgcggtct tcgcgtggcg gtgctgggcg gcggcgacaa tgccttcgag
     6661 aatgcgctgt acgccaccgg gcacggtgcc gcggcggtgc gactcttcgc gcggacggtt
     6721 cgcgcgcagc gacagctggt gcgcaaggtg ccggtggcgc aagtggtgct tggcgattat
     6781 gtcgtggatg ccggcgcccg gacggtgaat ggcgaagcgt tcgatctgat tctcgtgctc
     6841 tatggctggg atccttgcgc cgaattcgcg cagacgctgg acttgcggcg cagcgagcat
     6901 ggtttcatcg ccacggatca ggtcaccacg ctcaccagcc accatctggt gtacgccatt
     6961 ggcgaagtgg cgcagcgtct gcacccgtgc gtggtgacgg cgatggccga cggcatcgcg
     7021 gccgccaagg ccattcaggc caggatcgag gccgatcgct gatatatttg cccttctgct
     7081 accttgctga gttggtgggc gcacagcata ccggcgggcg ttgtttcaat cgcaggttcc
     7141 cggcgcggaa tgatgccggc agagccggcc ctcgtggcta atcccgctgg gtcgcgcgcg
     7201 tgacgatgta ttcggtcggc gcgggcgcgg ccagtgtcac cgtgaagtga cgcaattcat
     7261 caccacggaa aacatgcacg gtgacagagt cgccgggcag atggcgatcg agcacccgat
     7321 ccaggctggc ctggttcgtg atgcgcaggc cgtccacggc ggtcaggaga tcgcccgccg
     7381 acaggcccgc cgcgtgcgcc gcgccgtcgt cgtccgccgt ggcgatgcgg gtttcgtcat
     7441 tgacgctgcg ggtgcggatg ccaagggatg gtcgaccctc gttcgccttg ccctccagcg
     7501 caaagccttg cgtggcgagc agcgtttcca gtggcacgtc gtcgcacccc tcaacatgcc
     7561 ggtcaaggaa gccgcgcacg tcgacgcccg ttgcatcgtg gatgatcccg gcgatggcgt
     7621 cttcgggcac accgaggccc ggtctggggc cagtgaaaaa gcgacgccca tagcggcgcc
     7681 agagcaggcg catgacatcg tccaggctgc gtcgcccctg tgtggcccgc cgtatggtca
     7741 agtccaggcc cagtgccacg agcgatccct tggtgtagta actgaccagg gcgttcggcg
     7801 aattctcatc ctgcttgtaa tagcgggtcc aggcgtcgaa cgaactctcg gccaccgtct
     7861 gtttgtgccg ccccgagttg cggcgcacgg cggtgaccgt cttgccgatg aggcgcaggt
     7921 atccggcctc atcgatcacg cccgagcgtt tcagcatcag gtcgtcgtag taagaggtga
     7981 aaccttcgaa cacccacagc agacgtgtgt gattggggcg cgacaggtcg tagggggcga
     8041 acgcggcggg cttgatgcgc ttgacgttcc aggtgtggaa gtattcgtgg ctcagcaggc
     8101 ccaggaacac ggcgtagcct tccggggtgt cggggcggcc ctgcgcgggc aagccgtggc
     8161 gcgatgtgat cagcgcagtc gaggcgcgat gctccaggcc gccatacccg tccgcggtga
     8221 ctgtggtcag gaagacgtag cggtttgtcg agtcgaggaa cggcgcttgc cgggtttcgg
     8281 gttcgaacag ttcaatttgc gttgcgcaaa tcctgcgggc gtccgtggcg atgcggtcga
     8341 gatccaggcg cggcgccgcg ccggtgaaca ccagttcgtg ctcggcgccg tgggcgcgga
     8401 agcgcgcgac ctgtggggtg ccgatttcca cggggtggtc aatcagggcg tcatagccgg
     8461 gcgcggcgta caggccgaac ccatggcgcc gggcggcatc gcgatggccc gttgcttcgg
     8521 gcaggctggt gtagacccgc cagcggcgcg tgtgcggcgg cgggcacagt tcgagcaggc
     8581 aaggctggcg ttcgaagccg tcggcgctca ggaaaaccga agcgccgttg aagaacgcgt
     8641 gggactcgtc caggtgcgcg ccgcgcaccg agagatccca ggcgtagacc gtgtattcga
     8701 catgcaatgg gccctggcac ggggcgcatc gccatgtgtg gctgtcggtt ttcgtgatgg
     8761 taatatgccc acttccactc cgcgcctgta tgctttgtat ctggcgcgag aagtcccgaa
     8821 tcaagtagct gcccggtatc cacgccggca ggcgcagggt ctggccgcgt ggccgaggcc
     8881 ggggaacgct gatccgtacc ttgaaaaggt ggccggccgg gtcgaagggt gtcaggctgt
     8941 agagaatcga attcttgtcc atttgaatag tgtaaaggag acggcatgaa cgcaccgctc
     9001 atcaaggtcg agacccgtgg acgggtcgga ctgctgacca tagaccggcc caaagtgctg
     9061 aacgcgctca gcaacgacgt catcaccgaa ctctacagcg ccttgcgggc tttcgacgcc
     9121 gatgacggta tcggggccat cgtgctcacc ggcagcgaca aggcctttgc cgccggcgcg
     9181 gatatcggtg ccatgaaaga ttggaccttc caggatgtct atggccagga ttatctgggc
     9241 cacgactggg aagcgctcaa gcgcatccgc aaacccatca tcgcggcggt gtccggttac
     9301 gcgctgggcg gtggatgtga actggccatg gcctgtgatt tcatcatcgc cggagaaaac
     9361 gcgaaattcg gccaacccga gatcaagctg ggtgtgattc cgggctatgg cggtacgcaa
     9421 cgcctgccgc gggccgtagg caaggccaag gccatggatc tcgtgctgac cgcgcgtttc
     9481 atggatgtca cggaagccga gcgcgcgggc ctggtgtcac gcgtggtgcc ggtcgagcgc
     9541 gtgctcgacg aagccctcga ggcggccgcc gtgatcgcct ccatgtcgtt gccgtcggtc
     9601 atgatggcca aggaatgcat caatcgggcc tacgaggcgc ctctgaatga aggtctgctg
     9661 ttcgagcggc gcaacttcca tgcactgttc gccacgcatg atcagaagga aggcatgggc
     9721 gctttcgtcg acaagcgcaa gcccgatttc aagcatcgct gattcagtcg ccacacgcgg
     9781 ttccccaagc ctttctcgtc aaagagggac gatccgtgtc gcttcgctgg cagcttgttc
     9841 cgggtgatcc gatggctgca aagccagggt tttcgaacct ttgcagccgc agatcaagcg
     9901 aacgttgtga atcccccgca aatcctgccg aatgtgttgc aaccaggcct gacaggttgc
     9961 ttacgcctaa aaaaacacgc tatactttgc gagtttgacg gttagcaaat ttatatttgc
    10021 ccggcgccaa attgtgcttt cggccaatac cgctttgccc catgtggtcg tggggtatgt
    10081 gcggctggtg aacctctcag gggttttagg gaaggccctt caagggttag caagggaatc
    10141 tctcaggggt ttgcgggtta cggggtcggt ggtgcgtctt gtctggctgc atgccaagac
    10201 aggctgggtg gcgccgcccg agtcgtcaag aatcaggttt ggccggtttg cgtttggtgg
    10261 cacgcttagc gtggatgctt gaatgcgcct gcggtaaata acagtaatgt ctggaacaaa
    10321 ggtaagccgg gtgaccgtgc ctgtcgatga tgtgtccgcg catggcgctg acggcggcga
    10381 cgtggccggt gtggggccag aggggcttga attcaatgac gcggatctcg ccgcgctaaa
    10441 tgcgggagcg ggacgaggca tagttcgtgg cttggtcgcg caggcgatca tgggcggctt
    10501 ggccgcgctg gtgtcgtggg gcgtggcggg ttcgttcgcg gggctctccg ccctggcggg
    10561 ggccgctgcg tacttcgttc ccaacgcgct gttcgcttcc cggttgttgc tgggttccgc
    10621 cggggggcgg cgccccggcc cactggcttt ttttgtgggt gaggcgctga agctggggct
    10681 ggcggtgctg ttgctggggc tggctgtccg tctggggcat gcctggctgg tctggccggc
    10741 attcctgctg ggtttgctgt gtgtgctgaa gggttatgtg ttgttgcttg cggtgcgcgg
    10801 gctgcggtag cggttgaaag gtagtgcaga gaaattcatg ggcttcacgc cctcggtaac
    10861 aaagctaaca ggatagattc ggatggctgc tcccatcggc gtttcgccgc aggctgaata
    10921 cattcagcat cacctgattc acttgaacaa cacgggtgcc aagcagatcg gtgtcgtcaa
    10981 tttcggcgtg gtcaattacg attcgctgtt ctggtcgatc ctcatgggca tcatcgtcgt
    11041 gtatttcctg tggcgcgcca cacgctcggt gaccaccggc gtgcccggcc ggttccaggt
    11101 ttcggtagaa atgctcatcg actgggtcga gaacgaagcc aagggcatca tcgccaacga
    11161 cgtgtcgcgg cgtttcatgg ctcccctggc gctgacggtg ttcctgtgga tcgcgctgat
    11221 gaacgcgctg gatctggtgc ccgtcgactt gctgggcctc atcttcaagg acaccggcct
    11281 gggtgcgaac catcacgacc tcctgtatta ccaccgcatc cttcccacgg ccgacctgaa
    11341 cgtgcccatg ggcatgtcgt tgggcgtgct gctcatgctg ttctactacg gtatcaagat
    11401 caaaggtgtc ggccacttca tcaaggaaat gttcatcgcg cccttccatg ggcacggtgt
    11461 gatggcgttg tgcctggcgc cgttcaactt cctgctgaac atcatcgaat acgcggccaa
    11521 ggcggtttcg ctgggcatgc ggctgttcgg taacatgttt gccggcgagc tcatcttcct
    11581 gctgatcgcc ctgttgggcg gtgcctggac gggcttcaat ggcatgagca tcagcctggg
    11641 catcggtcac gtcatcgcgg gttcgatgtg gtccatcttc cacgtcctga tcgtgctgct
    11701 gcaggcctat atcttcatga tgctgaccct ggcgtacgtg gggctggctc acgaaaccca
    11761 ttgatagttt ttccggttcg ggcgggtcgg cccggccggt cgcaaggttt tcttgcaaaa
    11821 tccgctgtct tttttgactt tttgattaca aggtttttaa ccagggagtt gttatgacca
    11881 acgttgcttt cgttgctcta gcttgcggtc ttatcattgg tctgggtgcc gtcggcgctt
    11941 gtatcggtat tggtgtgatg ggtagcaagt atctggaagc ctcggcacgt cagccggaat
    12001 tgatgaacgc gctgcaaacc aagatgttcc tgctggctgg tctgatcgac gccgccttcc
    12061 tgattggtgt cggtatcgcc atgttgttcg ccttcgccaa tccgttcgtc gggtaagcga
    12121 ctgggcccgc gcttggcgcg ggttgagcag ccatcggggc gcgtgcgcat tccggtggct
    12181 tgaatcggac gtgggtgcgt atcgcgccag gcgtccgcgg caataaaggg aaacgactgt
    12241 gaacattaac gcgactctct tcttccagat gatcgtgttt ttcgttctgg gctggtttac
    12301 gatgaagttc gtatggccac ccatgacgaa ggcgatggat gagcgccgtc agaaaatcgc
    12361 cgatggtttg gcggcggcag agaagggcaa ggccgatctg gctcaggcgc aggcgcgcgt
    12421 cagcctgatc gaggcgtcgt ccaagtctga aacgcatgcc cgtctcgtcg acgccgaaaa
    12481 gcagggtgca ttgttgatcg acgaggcccg ccgtgaggcc gaagctgaaa aggcgcgcat
    12541 catcgcgcag gctcagcagg aagcgcggca ggaaatccag cgtgtccgtg aggggctgcg
    12601 cgccgatgtg gcgttgttgg ccgtcaaggg cgccgagcag atcctcaggc gcgaagtcga
    12661 cgcgaaggcg catgccgagc tgctcgaccg gctcaaggca gaactctgat caaggcaggc
    12721 catggctgaa ctatcgacta tcgctagacc ctacgccgag gcactgttcg ccgcggccaa
    12781 cgaagatccg gccgggctgg cgtcctggtc ggcgctcgtc gacgagctcg cccaggtggc
    12841 cggcctgtcc gacgtgcgcg aggcgctcac cgatccgcgc ctgaccaacg ccgctcgcat
    12901 cgagctgttc gtcgggctga tcaaggcgcc gttgtcccag tgggcccgga atttcgtgga
    12961 gctgctggtc aacaaccacc gtattctggt tttgccccag atcgccgagc agttcgaggt
    13021 gctgaagaac cagcatgaag gcaccgcgct cgctcagatc accagcgcgt tcgcgctcga
    13081 cgacaaacag gtgcgagagc tcatcgccgg gctcgagaaa aaattcggcc tgaaactcaa
    13141 gccatccgtc accaccgatc ccagcctgat cggcggcgtg cgggtggttg tcggcgacca
    13201 ggtgcttgat acttccgtgc gggcgcagct ggtccgcatg cgcgataagc tggccgccta
    13261 gagcgtgccc cacaacagga ttccaggagt ctgaacatgc aacttaaccc gtcagagatc
    13321 agcgagctgc tcaagagccg catcgaaggc ctgggtgcct ccaccgacgt gcgcacgcag
    13381 ggcacggttg tttccgtgac cgacggcatc acccgcatcc acggcctgtc cgacgtgatg
    13441 cagggcgaaa tgctcgagtt cccgaacaac gtttacggcc tggcgctcaa cctcgagcgt
    13501 gactcggttg gcgcggtgat tctgggtgac tacaccggtg tgtccgaagg cgacccggtc
    13561 aaaaccaccg gccgcattct cgaagtgccg gttggccccg agctgcgtgg ccgtgtcgtg
    13621 gatgccctgg gcaatcccat cgacggcaag ggccccatca acgccaaggc caccgacgtc
    13681 atcgaaaagg ttgcgcccgg cgttatcgcc cgccagtccg tgacgcagcc gctgcagacc
    13741 gggctgaaag ccatcgactc catggtgcca atcggccgtg gccagcgcga actcatcatt
    13801 ggcgaccgcc agaccggcaa gaccgccgtg gccatcgatg ccatcatcag ccagcgcggc
    13861 aagggcgtca cgtgcgtata cgtcgccatt gggcagaagg cgtccaccat cagcaatgtg
    13921 gtgcgcaagc tggaagaata cggcgcgctg gaatacacca tcgtcgtggc cgccgcggcg
    13981 tccgactcgg ccgccatgca gtatctgtcg gcctacgccg gctgcaccat gggcgaatat
    14041 ttccgcgacc gtggcgagga cgccctcatc atttacgacg atctgaccaa gcaggcctgg
    14101 gcctatcgtc aggtgtcact gctgctgcgc cgtccgcccg gccgcgaagc ctaccctggc
    14161 gacgtgttct atctgcactc ccgcctgctc gagcgtgccg cgcgcgtcaa cgaagattac
    14221 gtcgagaagt tcaccaaggg cga
//
LOCUS       DASTNK010000026        36836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1311294, whole genome shotgun sequence.
ACCESSION   DASTNK010000026 DASTNK010000000
VERSION     DASTNK010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 36836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36836
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311294"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /gene="purD"
                     /locus_tag="VFR20_01885"
     CDS             complement(<1..821)
                     /gene="purD"
                     /locus_tag="VFR20_01885"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU0229298.1"
                     /translation="MKLLVIGGGGREHALAWRLAQSPRVQKVYVAPGNGGTDNAEHLE
                     NVALATPEDLLRFAKDHDVAYTVVGPEAPLAQGVVDTFRKAGLKIFGPTKAAARLESS
                     KDFAKAFMARHRIPTAAYQTFTDAANAKAYIQAQGAPIVVKADGLAAGKGVVVAETVD
                     EACAAVDHMLGMGDGARVVVEACLQGEEASFIVMCDGKSILPLATSQDHKRLLDHDQG
                     PNTGGMGAYSPAPVVSPALHNRIMREVINPAVHGMAREGIPYTGFLYAGLMIGAGP"
     gene            complement(878..1606)
                     /locus_tag="VFR20_01890"
     CDS             complement(878..1606)
                     /locus_tag="VFR20_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0229299.1"
                     /translation="MAGHSKWANIQHRKGRQDAKRGKLWTKIIREITVAARAGGADPD
                     ANPRLRLAWDKATGANMPKDTIQRAITRGAGGADGASYEEVRYEGYGVGGAAVMIDCM
                     TDNRTRTVAEVRHVLSKNGGNMGQEGSVSFMFKHCGQFVFAPGTSEDSVMEAGLEAGA
                     EDIQTDDEGVIEVVCAPADYPAVKASFDAAGLVPETQGLTMKALSETELTGDDAEKMQ
                     KILDALEELDDVQDVYTTAVFDEA"
     gene            1714..3210
                     /locus_tag="VFR20_01895"
     CDS             1714..3210
                     /locus_tag="VFR20_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HEU0229300.1"
                     /translation="MANPILIAKASGTELALLPQLANRHGCVTGATGTGKTVTLQVLA
                     EAFSRLGTAVFLADVKGDLTGISQAGEASPKLLKRLEQHGLPVPAWGGCPAALWDVFG
                     EQGHPIRATVSDMGPLLLARMLELNDTQEGVLNLVFKVADDEGMLLLDMKDLRAMLQN
                     VADRAGELRARYGNVSVVSVGAIQRGLLALESQGGDRFFGEPMLDLADMIRTHPDGRG
                     VVNILAADKLMQAPRLYAVFLLWMLADLYESLPEVGDPEKPRLVFFFDEAHLLFNNAP
                     KALLEKIEQVVRLIRSKGVGVYFVTQNPLDIPDTVLGQLGNRVQHALRAFTPRDQKAV
                     KTAAQTMRPNPGLDLEAAITELGVGEALVSLLDEKGRPGVTQRAWMLAPASRIGPATE
                     AERAMLRADSPLGAKYDKAIDRESAYETLSARVQGDPEPGAQGAPESTAESGDGGVMG
                     AVGEFLFGSTGPRGGHRDGLVQSTLKSVVRREASQLLRGVLGSLMRRR"
     gene            3324..4265
                     /locus_tag="VFR20_01900"
     CDS             3324..4265
                     /locus_tag="VFR20_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HEU0229301.1"
                     /translation="MTVKAIVATQSADNTRRWCQAFRNAWPEARFDAWSESSPVPSGA
                     RYAIVWQPPSALFRNETALEAVFNLGAGVDSLLRLPELPRSLSIVRLEGAGMAEQMVE
                     YVLYGLLRAAREFSNYAVSQADVRWQTRPAIRREDWPVGVMGLGTIGTEVAQAVARLG
                     YPVAGWSRTARPVPGIEIFGGQENLAAFLARTRVLVNVLPLTPDTEDILNRDNLSRLL
                     PDGYLINVARGRHLVEEDLLALLDGGRLMGAMLDVFRTEPLPADHPFWRHPGIVVTPH
                     VAAITLQDDTVAQIVGKIRDMERGVAVSGVVNRGSGY"
     gene            4289..5359
                     /locus_tag="VFR20_01905"
     CDS             4289..5359
                     /locus_tag="VFR20_01905"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEU0229302.1"
                     /translation="MSTLHKIAVMPGDGIGKEVVPEGLRVLEAAASRFDIGFDWDHLD
                     WGCDYYAAHQRMMPDDWKERIGSHEAIFFGAIGWPDTVPDHVALWGSLFRFRREFDQY
                     INLRPVRLMPGVTSPLAGRAPGDIDFFIVRENTEGEYSNSGGRLFEGTPREVVIQESV
                     FTRAGAERVLKFAFELARKRGRHLTAATKSNGISISMPWWDERVADMASQYPDVRWDK
                     YHIDILCAHFVQHPDWFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERRFPSL
                     FEPVHGSAPDIYGKGIANPIGQIWSGALMLDFLGYPDAAQAVVTAIEAVLIEGPRTPD
                     MKGNASTSDVGKAIAERVAHES"
     gene            5381..6370
                     /locus_tag="VFR20_01910"
     CDS             5381..6370
                     /locus_tag="VFR20_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEU0229303.1"
                     /translation="MLSRYVKAIRVDEHGGPEVLKLVSVELSAPQDDEVTIDHKAIGL
                     NFVDIYWRTGLYARPVPHGMGFEGAGVVSAVGPKVRHLKVGDRVAYGQSPIGAYAEAH
                     NVPERLVVKLPKSVGFDEAAALMLKGLTVQYLFRQTYRLQGYETILFHAAAGGVGLIA
                     CQWAKALGVKLIGTTSSPEKAALAKAAGAWEVIDYTRENVVERVLELTGGKKVPVVYD
                     SVGKDTWMTSLDCLQPRGLMVSFGNASGPVDGVNLGILASKGSLYVTRPTLGTHVLAR
                     EQLVEAADELFGLVTKKKIVVNIGQRFPLARAADAQIALAGRKTTGATVLTVD"
     gene            complement(6425..7447)
                     /locus_tag="VFR20_01915"
     CDS             complement(6425..7447)
                     /locus_tag="VFR20_01915"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEU0229304.1"
                     /translation="MKRITLTQYLVEQQRQKQAVSAEVRLLIETVARACKAIGHAVGK
                     GALGGVLGSLDTENVQGEVQKKLDVLSNEILLEANEWGGHLAAMASEEMETIHRIPNR
                     YPKGEYLLLFDPLDGSSNIDVNVSIGTIFSVLHAPHHASGRDVDESDFLQPGSRQVAA
                     GYAIYGPQSMLILTVGTGVVAFTLDREMGSWVLTEANLTIPEDTAEFAINMSNMRHWH
                     APVRRYIDECLAGKEGSRGKDFNMRWIASMVADVHRLLTRGGIFIYPRDQRASTRNGK
                     LRLMYEANPMSFIVEQAGGCAIDGADRILDIQPHALHQRVGVALGSKNEVERLRAYHA
                     EATQNA"
     gene            complement(7490..10177)
                     /gene="pepN"
                     /locus_tag="VFR20_01920"
     CDS             complement(7490..10177)
                     /gene="pepN"
                     /locus_tag="VFR20_01920"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEU0229305.1"
                     /translation="MPDQTGKTISRHNYRPYSHTLDHVALHFTLDPAETRVRATLRFH
                     GPVDAPLVLDGRDLTLESIKLDGQPLGPAQYQLDDEHLTLQPASDTFELEIISLCRPA
                     DNASLMGLYVSGASLFTQCEAQGFRRITWFPDRPDVMATYRVTLEADKARFPLLLSNG
                     NLIQARELPEGRHEAIWEDPHPKPCYLFALVAGDFSVRERTVKTRGQRDALLQIYSDR
                     GSEDKTGWALDSLQHAMEWDEHRFGLELDLDRFMVVAARDFNMGAMENKGLNIFNSAY
                     VLADPDTATDASYRSIESVIGHEYFHNWTGDRVTCRDWFQLSLKEGLTVFREQEFSAD
                     MMAQGLDDIQALSARAVKRIDDVSMLRLAQFPEDAGPMAHPVRPESYQEIANFYTATV
                     YEKGAEVIRMQHTLLGETGFRAGMDEYFRRHDGNAVTCDDFVDAMESVYVRQNPGKNL
                     DVFRRWYSQAGTPRVTVALRHDKGSQTCEVTLSQHCAPVGVEKLANPPLDKQPLHIPF
                     ALGLLGANGQPLPIGHDGRVTDTVLLELTETSRTWTLTGIPEQPIPSLLRNFSAPVIV
                     DYDRPDADLALLARHDTDPFARWEACQELATRQLLALVRAGQSGSTLSVDQAFIDTWR
                     TLLQDESLTPAYRTRALSLPPEKIILEKTAPMDPLAVTQARDHLRQVLGASLAPLWLD
                     AYSRHTDHDDNYRIDTVSAGRRALKNLALVYLMAGGHADGARQARDQYFGARNMTDRM
                     GGLSALVNFGDVEDAGPALEHFYDQWQHDPLVIDKWFALQATAPSTRVETVRALMLHP
                     AFTLRNPNRARSLLFQFCMNNLRAIHAPEGYPFWAEQVIAIDRFNPEVAARLARAFDN
                     WTRFAQPHRDSMEQALRHIQAQPALSRNVSEIITKSLSL"
     gene            10390..11019
                     /locus_tag="VFR20_01925"
     CDS             10390..11019
                     /locus_tag="VFR20_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEU0229306.1"
                     /translation="MSTGYQGRSGALRKLARASIILVAACFLAGCATTLSARVTRFEK
                     WPANAVGATYRLVPGAGQSNNLEYQAFADTMRAAIGPTGLVEARSGALARFDVSFSYV
                     ATKGVEWMTRYADFPPYAPWGPWGYYGGGPFGPPMVNVPVAVYRNELIVIIKDRSQND
                     AEVYRSSAVTNTASKDLPQVMPYLARAVFDHFPGNNGQVMVVRYTLGGR"
     gene            complement(11179..11541)
                     /locus_tag="VFR20_01930"
     CDS             complement(11179..11541)
                     /locus_tag="VFR20_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HEU0229307.1"
                     /translation="MSRDTYSALQAKIEKEITKLQKQASALRIKRRVPVIASIVRTMR
                     EYDITPEEIAQALNKKAVKVASPKANGAASAAKRSVPIKYRHPETGETWTGRGKAPRW
                     IIKAESDGKQRDAFLIEQ"
     gene            11962..12786
                     /locus_tag="VFR20_01935"
     CDS             11962..12786
                     /locus_tag="VFR20_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEU0229308.1"
                     /translation="MNSNSIPDDFRVAAVQTVSSTSVDGNLKMAADLIGQAAETGARL
                     VVLPEYFCLMGHRDGDKFDIREKPGAGPIQEFLRSQARHHGIWLVGGTLPLESPDAGR
                     IYNSSLVFSPDGAQVARYDKIHLFSFHRGDESYDESVAIRPGDNPPQVFSASCGRVGL
                     SICYDLRFPEFFRALGDLGLIVMPAAFTYTTGKAHWEILLRARAIENQCYVLASAQGG
                     KHENGRRTWGHSVLIDPWGEIVDVLPEGPGVVSGMIDAGRLSDVRQSLPALKHRTM"
     gene            12908..14365
                     /gene="tldD"
                     /locus_tag="VFR20_01940"
     CDS             12908..14365
                     /gene="tldD"
                     /locus_tag="VFR20_01940"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEU0229309.1"
                     /translation="MKVSDPAISALATAKSVLLDPWGLSEADIARALGEIFTHKVDYA
                     DLYFQYTRAEGWSLEEGIVKTGNFSISQGVGVRAVSGDKTAFAYSDALSADALLSSAR
                     AVRSIARQGAGRRKVARPGNGRPHDLYRDLDPVDSLSAPDKVALLGRVEAMARAADPR
                     IIQVMAGLGAEYDVVLVAGSDGRLAADVRPLVRMSLTVIAEQGGRREMGHGGGGGRTG
                     LACFTDEALRGYVRQAVHEALTNLEARPAPAGEMTVVLGSGWPGILLHEAVGHGLEGD
                     FNRKGSSVFSGRIGEQVASKGVTVVDDGTLAERRGSLNIDDEGNPTQCNVLIEDGVLK
                     GYMQDSLNARLMKTAVTGNGRRESFAHLPMPRMTNTYMLAGSEAPEDIIASVDNGLYA
                     ANFGGGQVDITSGKFVFSASEAYLIKNGRVTHPVKGATLIGNGPDAMTRVRMIGNDLR
                     LDSGIGTCGKEGQSVPVGVGMPTLRIDGLTVGGTS"
     gene            14685..15224
                     /locus_tag="VFR20_01945"
     CDS             14685..15224
                     /locus_tag="VFR20_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0229310.1"
                     /translation="MTNSSQNTQQYDLRILRALRRLTRSIALHSRQLAACSHITAPQL
                     ICLRAVIDHGPLTATAISREVHVSASTVVGILDRLEDKGLVRRERGREDRRIVFVSAT
                     EQGVALARETPSPLQKKLADALNALPELEQATITLSLERVVELIESGAPELRDVPGDA
                     SPLLDVPTDGALPESGLVV"
     gene            15239..15781
                     /gene="ectA"
                     /locus_tag="VFR20_01950"
     CDS             15239..15781
                     /gene="ectA"
                     /locus_tag="VFR20_01950"
                     /EC_number="2.3.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="HEU0229311.1"
                     /translation="MDVVCEQETLDDTTQVAEAPLILRAPGKHDGRAIHDLVAHCAPL
                     DLNSVYTYLLLAEHFSPTCVLAHAQDRLRGFVSAYVPPGRPDVLFVWQVAVHRQVRGR
                     GVGPRMLRHLLERPGLEDVRYLETTVGPSNRASRSMFARVAGSLGASVRESRLFSRSL
                     FGPHGHDDEPLLRIGPFDAH"
     gene            15795..17102
                     /gene="ectB"
                     /locus_tag="VFR20_01955"
     CDS             15795..17102
                     /gene="ectB"
                     /locus_tag="VFR20_01955"
                     /EC_number="2.6.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEU0229312.1"
                     /translation="MDLKIFERMESEVRGYIRSFPVVFTQARGSLLVDEDKNEYADFF
                     SGAGTLNYGHNNPLLKRRLLEYLETDGVVHGLDMATSAKKYFLETFERTLLAPRGWSY
                     KLQFTGPTGTNAVEAALKLARQVKGRPNIISFTHGFHGVSGGSLSVTANSKFRQAAGF
                     SLANTTFMPYDGYLGPDVDTMTYLERMLDDPSSGLDHPAAVIVETVQGEGGVNVASWR
                     WLKDLERLCRRHDMLLIVDDIQVGCGRTGNFFSFEAAGIQPDIITLSKSLSGFGLPMS
                     LVLFRPELDIWKPGAHSGTFRGNNLAFVTAAQALESYWSDTTLANDTARKERLVRDWL
                     ENLAHSYRGAGLSVRGRGLIQGLVTPRSGDVANRVAHEAFERGVVIETSGAHDEVLKL
                     LPALTMDDDLLRRGLEVIESAVGSVMRQSPTNVRVLEAGGKNR"
     gene            17099..17497
                     /locus_tag="VFR20_01960"
     CDS             17099..17497
                     /locus_tag="VFR20_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine synthase"
                     /protein_id="HEU0229313.1"
                     /translation="MIVKNVNDVIGTRDEVKTETWLSRRVLLKQDGMGFSFHETVIFP
                     GTETHIHYCNHLEAVWCIEGDGEIETVADRRKYTLGPGVVYALDEHDEHWLRGGTRPL
                     RVICVFNPPLTGQEVHDSNGVYPAAQPASA"
     gene            17512..18432
                     /gene="thpD"
                     /locus_tag="VFR20_01965"
     CDS             17512..18432
                     /gene="thpD"
                     /locus_tag="VFR20_01965"
                     /EC_number="1.14.11.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine hydroxylase"
                     /protein_id="HEU0229314.1"
                     /translation="MNTPVSDVYPSRSDRDAAIIARQDPVVYGMGSYAEALAPERLAA
                     YERDGFLLVPDLFPHEEVQALSAEIERMKADPAMAALDETIAEPRSQVIRSIFRVHEL
                     SDIMAGLVRDPRVADVARQILGSEVYVHQSRVNLKPGFEGREFYWHSDFETWHVEDGM
                     PRMRALSCSILLTDNTACNGPLMLMPGSHRQFVSCRGRTPDEHYRMSLRKQEYGVPDQ
                     ISLRLLAEQGGVEAMTAGAGSVVFFDCNTMHGSSGNISPLSRANVFVVYNSVDNALRE
                     PRHGLKPRPEYIAARQSCDPVRSHEAASAA"
     gene            complement(18530..19312)
                     /gene="ehuA"
                     /locus_tag="VFR20_01970"
     CDS             complement(18530..19312)
                     /gene="ehuA"
                     /locus_tag="VFR20_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0033308 - hydroxyectoine transport
                     [Evidence IEA];
                     GO_process: GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine/hydroxyectoine ABC transporter
                     ATP-binding protein EhuA"
                     /protein_id="HEU0229315.1"
                     /translation="MNDDAPIIKFDNVVKRFGSVTILDSLNFNVRKGEKVTIIGPSGS
                     GKSTVLRILMTLETINEGFIYVDGKPLWHEEKNNQLVPASEAHLRTMRKEMGMVFQQF
                     NLFPHMTVRRNVTEAPMHVLKLSKEKAARRAEKYLDLVGLSDQANKFPSQLSGGQQQR
                     VAIARALAMHPNIMLFDEPTSALDPELVGEVLNVIQRLAGEHDLTMLLVTHEMQFAKQ
                     ISDRVCFFDKGRIVEEGPPQEIMVSPREARTQEFLKGYIQRA"
     gene            complement(19309..20001)
                     /gene="ehuD"
                     /locus_tag="VFR20_01975"
     CDS             complement(19309..20001)
                     /gene="ehuD"
                     /locus_tag="VFR20_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0033308 - hydroxyectoine transport
                     [Evidence IEA];
                     GO_process: GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="HEU0229316.1"
                     /translation="MLFGIHWDTTNSWTLASSIFPILLRGLVVTIEATIVGFLVAAIL
                     GLILAALKSARSRFISWPARLITEFLRDTPLLIQLFFLYYVLPDYGIVLPAFLTGALA
                     LGVQYSAYMSEVYRAGLESVAHGQTEAARALDFTALRTFTIIVLPQAIPRIIPALGNY
                     LVSIMKDVPVLSVVSVLEMLNVAKIIGDRTFDYMVPLSMVGGLYMVLTLVASAGVRFL
                     DTHLPKQGLPLR"
     gene            complement(20003..20674)
                     /gene="ehuC"
                     /locus_tag="VFR20_01980"
     CDS             complement(20003..20674)
                     /gene="ehuC"
                     /locus_tag="VFR20_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0033308 - hydroxyectoine transport
                     [Evidence IEA];
                     GO_process: GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuC"
                     /protein_id="HEU0229317.1"
                     /translation="MNWTMYSPPLLQGAWVTIQLTFYSTVLGGIFSFAFGVGKLSRNW
                     AAKGVSIFFIEVFRGTSLLVQLFWLYFALPIFGQAIGLDLRLPPVIAGTLALSLNIGA
                     YGAEVVRGAIQAVHPNQHEAARALSFTPRQTLWRISIPQAIPEMMPTFGNLAVQNLKD
                     TALVSLISLGDLAFRAEQIRTFTQDSTTVYTLLLFMYFGMALVLTAIMQLLERSVGRW
                     RRGRS"
     gene            complement(20881..21744)
                     /gene="ehuB"
                     /locus_tag="VFR20_01985"
     CDS             complement(20881..21744)
                     /gene="ehuB"
                     /locus_tag="VFR20_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033294 - ectoine binding [Evidence IEA];
                     GO_function: GO:0033295 - hydroxyectoine binding [Evidence
                     IEA];
                     GO_process: GO:0033308 - hydroxyectoine transport
                     [Evidence IEA];
                     GO_process: GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine/hydroxyectoine ABC transporter
                     substrate-binding protein EhuB"
                     /protein_id="HEU0229318.1"
                     /translation="MKKTYRTALLALSAASLALGIANQTNAETLKQIKDRGYIRIAVA
                     NEIPYGYIDPSGNAKGAGPDVATQVMKQLGVKEIQWVATNFGSLIPGLLANRFDMVAA
                     EMAVLPPRCKKVIFSEPDSSYGEGLLVAKGNPDNLHSYEAFAKNGKKIAIMAGADQLE
                     MLQALKVPAGQMVTISSNSDAISTVATGRAAAYAATSLTASQLAAKSQKVEVAKNFKD
                     PVVDGKPVRSWGAFVFSKKSTGLRDAVNKQLAKFKKTPAWSKIMTQYGFTEADNKASF
                     EKTTSKLCSGG"
     gene            22694..23788
                     /gene="aroG"
                     /locus_tag="VFR20_01990"
     CDS             22694..23788
                     /gene="aroG"
                     /locus_tag="VFR20_01990"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEU0229319.1"
                     /translation="MSHNTDDLRIREIKELSPPAHVMREFVCTPEISATVYGARRGLH
                     QILHGEDDRLAVVIGPCSIHDPRGALEYAGRLAEQRRRFAGELEIIMRVYFEKPRTTV
                     GWKGLINDPGLDGSFNINQGLRTARELLIDINGMGLPAGTEFLDMITPQYIADLVSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGNIKIAVDAINAASQPHHFLSVTKGGHS
                     AIVSTLGNNDCHIILRGGKQPNYDAASVEAACQFLVKSGLRTQLMIDASHANSNKDYR
                     NQPSVIDAVAAQVEGGDSRISGLMVESHLVGGRQDLVPGRAPVYGQSITDGCVDWDTS
                     VRILERLAKAVEARRRVAKVGAVEDSPAAA"
     gene            23914..25338
                     /locus_tag="VFR20_01995"
     CDS             23914..25338
                     /locus_tag="VFR20_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEU0229320.1"
                     /translation="MNAPMAGQEVRRPIPDGFFDSFEQKFGSRFSRARAVREHHGRDE
                     SPYPPMLPDAVVYASTTDEVAWLARHCNQHGVPLIAYGVGSSLEGHLLAVQGGVSVDL
                     SGMNQIVSIDAEDLTATVQAGVTRKQLNEALRDTGLFFPIDPGADASLGGMSATRASG
                     TNAVRYGTMRENVMNLKVVTADGRVIETANRARKSSAGYDLTHIFVGSEGTLGLITEV
                     TVRLYPQPEAISAAICNFPTLDSAVQSVIEIIQMGIPVARVEFMDTAAVRATNAYSKL
                     TLRESPLLLFEFHGSAAAVKEQAEAVQGITHDNGGMDFEWAEHPEDRSRLWTARHNAY
                     FAGLGLRPGCRSSTTDVCVPISRLAQCVTETAAELDRASFPYTIVGHVGDGNFHVLML
                     LDPDSSAEWDESERINNSLVRRAIAMDGTSTGEHGVGLHKMEFMLAEHGQDALDLMRS
                     LKQAFDPNNILNPGKIIPPRAGAR"
     gene            25392..26828
                     /locus_tag="VFR20_02000"
     CDS             25392..26828
                     /locus_tag="VFR20_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEU0229321.1"
                     /translation="MARLGQAIPSHCILSRVEDTRPYECDGLSLYRALPPVVVLPENE
                     AQIIEVLNACRQFQVPIVARGAGTGLSGGAMPHARGILLGTAKLNRILRIDTSSATAV
                     VQPGVRNLAISEAAAPHGLYYAPDPSSQIACSIGGNIAENSGGVHCLKYGLTVHNVLR
                     VRMVTIEGEVVELGSEAPDSPGLDLLAAFIGSEGMLGVVTEATVKLIPKPACAQVIMA
                     SFSSVEAAGQAVTDIIAAGIIPAGLEMMDQRAVHMVEPFVKAGYDLDAAAILLCESDG
                     TTDEVRDEVARMEAVFRRAGATRLQVSRSEQERLLFWAGRKNAFPAAGRMSPDYYCMD
                     GTIPRRHLARVLGAIEQMEDEYGLRCANVFHAGDGNLHPLIMFDSNKPDEVDRAEKFG
                     AAILELCVQVGGTVTGEHGVGIEKINQMCVQFGREELDAFLALKSAFDTEGLLNPEKV
                     IPTLARCAEYGKMHVHGGDIRFPELPRF"
     gene            26838..27926
                     /gene="glcE"
                     /locus_tag="VFR20_02005"
     CDS             26838..27926
                     /gene="glcE"
                     /locus_tag="VFR20_02005"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEU0229322.1"
                     /translation="MEFVLTELIERVMTARAAQRPVHIRGGGTKDFYGEPGSLQGATN
                     HSVLDMSAYHGVVNYQPPELVLTVRAGTRLAEVERLLDEHGQMLAFEPPRFGPDSTVG
                     GCVAAGLSGPRRAAAGGLRDFILGAKLLDSSGSVLEFGGEVMKNVAGYDMSRLLAGSM
                     GIFGALVEVSIKVVPKPLVEKTARLDVDEAGALALFNQWRGKPLPVSATAWVPGEAPA
                     LWVRLSGSAPAVDAGARVVGGTEADSDEAGPFWDSLRDHTHPFLRSCPLWRLAVPPQV
                     DPLGLGPSLIEWNGGQRWLAQSLEAGALRERVARLGGHATLFRPGADNNAPVFHPPAP
                     GLMAISRRLKHALDPVGIFNPKRLFSEF"
     gene            27936..29174
                     /gene="glcF"
                     /locus_tag="VFR20_02010"
     CDS             27936..29174
                     /gene="glcF"
                     /locus_tag="VFR20_02010"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEU0229323.1"
                     /translation="MQTHLAPWAQDTNFGQDADAILRRCVHCGFCLATCPTYQILGDE
                     RDSPRGRIYLIKQVLEGAELTGETQEHLDRCLTCRNCETTCPSGVEYGALVDIGRNLV
                     DRKVERPFRQRVSRALLRQTLTSRWFGTAFRLGRLLRPLLPEVLKGKIPAARPAGKLP
                     SKTSYARTVLMPVGCVQPAMMPSIDAATLRVLDALGIGVRMEPGSGCCGAINFHLDAQ
                     DQARGQMRANIDAWLPLLENGTVEAIVINASGCGAMIKEYAHHLKHDPAYAGKAQRLV
                     EKVVDVAEIVAPHAVELAPRLHGLPARVAFHPPCTLQHWQGLRGVSERLLTDLGFNLV
                     SFSESHLCCGSAGTYSVTQPELAKSLRARKLGHIQEVRPDSIVSSNIGCIGHLQGGTS
                     VPVRHWIEIVDEALAASKAN"
     gene            29222..30337
                     /gene="mnmA"
                     /locus_tag="VFR20_02015"
     CDS             29222..30337
                     /gene="mnmA"
                     /locus_tag="VFR20_02015"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU0229324.1"
                     /translation="MSTVSVSKGRVVVGMSGGVDSSVSAWLLKQQGYDVVGLFMKNWE
                     DDDDSEYCSSRQDWLDAASVADVVGVEIEAVNFAAEYKDRVFAEFLREYSAGRTPNPD
                     VLCNAEIKFKAFLDHAMSLGADHIATGHYARVRAISTGHSTEYQLLKALDASKDQSYF
                     LHRLNQSQLAATLFPLGDMPKTEVRRIAREIGLPNAAKKDSTGICFIGERPFREFLSR
                     YLPTRPGPIKTPEGAVIGMHQGLSFYTLGQRKGLGIGGVKGRQRDDGTADAWYVARKE
                     LDTNTLYVVQGHDHPWLLSDTLGAQDASWVAGCAPAPGGYTAKTRYRQADAGCSLNAD
                     ASNGFSLRFSQAQWAVTAGQSAVLYDGEVCLGGGIIY"
     gene            complement(30379..30993)
                     /locus_tag="VFR20_02020"
     CDS             complement(30379..30993)
                     /locus_tag="VFR20_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0229325.1"
                     /translation="MKLIGSLSSPYVRKVRVVMAEKKLDYEFVLEDVWADNTRISQHN
                     PLGKIPCLIMDDNGSLFDSRVIVEYLDTLSPVGRLIPPQGRERAAVKCWEAIADGLLD
                     ATAAIHIERTKRAPEHLSEAWIARQRKKITAALEFMDKGLDEHPFCMGVNFSLADVAV
                     GCALGYLDLRLPDLDWRSQHSNLQRLNEKLSARPSFISNEPPKP"
     gene            31370..32746
                     /gene="purB"
                     /locus_tag="VFR20_02025"
     CDS             31370..32746
                     /gene="purB"
                     /locus_tag="VFR20_02025"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU0229326.1"
                     /translation="MQIADSLSSLNALSSLDGRYASKVGALRESLSEAGFMAHRVEVE
                     IAWLEGLAELGLPELSAFSSRAKAFLADLVRSFSEADAARIKEIERTTNHDVKAVEYW
                     LKERVAGDAELSAAAEFIHFACTSEDINNTSHALMLTRARENVLLPRLGEICALLKAF
                     ARQFADQPMLARTHGQPASPTTLGKEFANVAARLERAIAAVSVVQPLAKMNGATGNYN
                     AHLSAYPDVDWPGFSARVLTGLGLTQNPYSIQIEPHDWMAALFDAVARANTIILDLDR
                     DIWGYISLGYFKQQLKEGEVGSSTMPHKVNPIDFENSEGNLGLANAVLRHLSDKLPVS
                     RWQRDLTDSTVLRNLGVAFGYSVVAWDACLRGLNKLELNAAAIDADIDGCWEVLAEPV
                     QTVMRRYGLPQPYERLKALTRGKGINREGLAEFIAGLDLPEAPKARLLALTPRTYVGL
                     AAKLAREV"
     gene            32812..32887
                     /locus_tag="VFR20_02030"
     tRNA            32812..32887
                     /locus_tag="VFR20_02030"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32845..32847,aa:Lys,seq:ctt)
     gene            complement(32990..34444)
                     /locus_tag="VFR20_02035"
     CDS             complement(32990..34444)
                     /locus_tag="VFR20_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEU0229327.1"
                     /translation="MFDIFSGLNFWIGTSLVLALGFVLTFEFINGFHDTANAVAMAIG
                     TKAITPHLAVMLSGIFNFLGVLTGGVGVAYAIVHLLPVELLVNIDTGHGLIMVFSMLA
                     AAITWNLGTWYLGIPASSSHTLIGSILGVGVANAWLTHATLAEGVNWGKAVEILLSLV
                     ISPLIGFALAALLQWAARRWKPDSKMHQTHDQRREAGKKHPPFWNRLVLVLSALGLSY
                     VHGSNDGQKGIGLIMLVLIGIVPAQFVLNLNATSYDIDSVRTAATHLTQIMAHRTPAP
                     LHQDPQAGAKTDTPACDPRNTAHTTSLLLHDLNGVNSYRDLTGKQRVVVRRYLLCLDN
                     IAKKVEDREDLSSPERADLARLRSDLTTTTEYAPLWVIAAVALALGLGTMVGWRRIVE
                     TVSEKIGKQTVTYAQAMSAQLTAMFSIGLANVFSLPVSTTHVLSSGVAGTMMANGSGI
                     QGRTVRNIVLAWVLTLPAAMILAAGLFLLGMRFV"
     gene            34677..36596
                     /locus_tag="VFR20_02040"
     CDS             34677..36596
                     /locus_tag="VFR20_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU0229328.1"
                     /translation="MTTTNDISMDPPVLPGVRQEGSTEGLPLAPDLSLSLDAAHMPRR
                     STLIDKSMTRIIVMSLILGVASGLIAKALTVLIGIVTNLCFYGRWSTDFSSPADAVPH
                     LGWWVVVIPVIGGLIAGFMARWGSRAIRGHGIPEAMEQILLHESRIPPRITFLKPISS
                     AIVIGTGGPFGAEGPIIATGGALGSLIGQIMKVTGSERKVLLAAGAAAGMAAIFSAPV
                     AAVVLAVELLLFELRARSLIPVALACVAATAVRYVLQGTAPVFLMPHFDSPGAMALVV
                     YVVIGALVGVFSVWTTHIVYAIEDGFEKLPVHWMWWPALGGLVVGVIGYFAPLTLGVG
                     YSNIEDIVSGRLTAGALVFLCVMKFISWSISLGSGTSGGTLAPLFTIGGALGGLIGIG
                     FVTIAPHLGVNPNVAALVGMAAIFAGASRALLTTVVFALETTGQTAGLLPLLGACTAA
                     YLVSALMMRDTIMTEKIARRGVKVPSEYLADYLDRISVGAVCERSVVTLKATQTLEDV
                     RRWMADGKPESQHQGYPVVGESGKLLGVLTRRTFFTEDVPAATPLGELIVRPPVAVSE
                     RNTLREAADHMAEQNVGRLLVLDGQNNKRVVGMLTRSDLISAHSRRLKEGHRRRRYLS
                     LKTYQHIRRHRARRD"
     gene            complement(36606..>36836)
                     /locus_tag="VFR20_02045"
     CDS             complement(36606..>36836)
                     /locus_tag="VFR20_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0229329.1"
                     /translation="GSAPAARSGTASALINVARIAGAALGVAVLGTLYATRHDPSSGL
                     RLAMMCGGVFQIACAILAWRITRSRNATDGRG"
BASE COUNT         6590 a        10918 c        11889 g         7439 t
ORIGIN      
        1 ggacccgcgc cgatcatcag cccggcgtac aggaagcctg tgtacgggat gccttcgcgt
       61 gccatgccat gcacggcggg attgatgacc tcgcgcatga tgcgattatg cagcgccgga
      121 gaaaccaccg gcgcggggga ataggccccc attccccctg tgttcggacc ctgatcgtgg
      181 tcgaggagcc gtttgtggtc ctggctggtg gccagcggca gaatgctttt gccatcacac
      241 atgacgatga aactggcttc ctcgccttgc agacaagctt cgaccacgac acgcgcgccg
      301 tcccccatgc ccagcatgtg atccactgcg gcacaggcct cgtcgaccgt ctccgccacc
      361 accacgccct tgccagccgc caggccatcg gccttgacga cgatgggagc gccctgggcc
      421 tggatgtatg ctttcgcgtt cgcggcgtcg gtgaacgtct ggtaggcggc cgtgggaata
      481 cggtggcgcg ccatgaacgc cttggcgaag tctttcgaac tttcgagccg cgcggccgct
      541 ttcgtggggc cgaagatctt caggccggct ttgcggaatg tgtcgaccac accctgggcc
      601 aacggggcct cgggacccac gaccgtatat gcgacgtcat ggtccttggc gaaacgcagc
      661 aagtcttcag gcgtggcgag ggcgacgttt tccaggtgtt cggcattatc cgtgccgcca
      721 ttcccggggg cgacatacac cttttgcaca cgtggggact gagccagtcg ccaggccagg
      781 gcgtgttcgc gtccgccgcc gccgattaca agaagtttca tggtgagaag gggaatcggt
      841 ttgttgtcat ggcgcaggtg cggcatggtc ggcaaaatca ggcttcgtca aagaccgccg
      901 tggtgtagac gtcctggaca tcatccagct cctcgagcgc gtcgaggatt ttctgcattt
      961 tttcggcatc gtcgccggtg agctcggttt cgctcagggc tttcatggtg aggccctgtg
     1021 tttcaggcac aagccccgcc gcgtcgaatg acgccttgac ggccgggtaa tcggccggcg
     1081 cgcacacgac ttcgatcacg ccttcgtcgt cggtctggat gtcttcggct ccggcctcga
     1141 gcccggcctc catgacgctg tcttcagagg tgcctggcgc gaagacgaac tggccgcagt
     1201 gcttgaacat gaagctcacc gagccttcct gccccatgtt gccgccgttc ttggacagca
     1261 cgtgccggac ttcggccacg gtacgggtgc ggttatcggt catgcagtcg atcatgacgg
     1321 ccgcgccgcc cacgccgtac ccttcatagc gcacttcttc atagctggcg ccgtcggcac
     1381 cgcccgcgcc gcgcgtgatg gcgcgctgga tggtgtcctt gggcatgttg gccccggtgg
     1441 ccttgtccca ggccaggcgc aacctggggt tggcatccgg gtcggcgccg ccggcccggg
     1501 ccgcgacggt gatttcgcgg atgatttttg tccagagctt gccccgcttg gcgtcctgac
     1561 gccctttgcg gtgctggata ttcgcccatt tgctgtgacc ggccatacga ttgcgtcaac
     1621 ctcattgaag aagacgcata ttttaacctg tgcaggggat cgtccgtcga attacacttg
     1681 gcctacatta tccgccacga ggagaagcac acgatggcca accccatcct gatcgccaag
     1741 gcgtccggca ccgaactcgc gctgttgccg caactggcca atcggcacgg ctgcgtcacg
     1801 ggcgccacgg gcacgggcaa gacggtcacc ctgcaggttc tggccgaggc gttctcccgt
     1861 ctcggcacgg cggttttcct ggctgacgtc aagggcgatc tcaccggaat ctcccaggct
     1921 ggggaagcct cgcccaaact gctgaaacgc cttgaacaac atggcttgcc cgtcccggca
     1981 tggggtggct gtccggcggc gctttgggac gttttcggag agcaagggca tccgatccgg
     2041 gccacggtgt ccgatatggg gccgctgttg ctcgcgcgca tgctcgagct caacgacacg
     2101 caggaagggg tgctcaacct cgtcttcaag gtcgccgacg acgaaggcat gctgctgctc
     2161 gacatgaagg atctgcgggc catgttgcag aacgtggccg accgcgccgg cgaactgcgg
     2221 gcgcgttacg ggaatgtgtc tgtcgtttcg gtgggcgcca tccagcgcgg gttgctcgcg
     2281 ctggaatcac aaggcggcga ccgattcttc ggcgaaccca tgctcgacct ggcggacatg
     2341 atccgcacgc acccggatgg ccgtggcgtg gtgaacattc tggcggccga caagctcatg
     2401 caggcaccac gcctgtacgc cgtgttcctg ctttggatgc tggccgatct gtacgaaagc
     2461 ctgccggaag tgggtgaccc tgaaaaaccc cgcctcgtgt tcttcttcga cgaagcgcac
     2521 ctgctgttca acaacgcgcc caaggcgttg ctcgagaaaa tcgaacaggt cgtgcggttg
     2581 atccggtcca agggcgtggg ggtgtatttc gtcacccaga atcccctgga cattcccgat
     2641 accgtgctcg ggcagttggg caaccgcgtc cagcacgcct tgcgggcttt cacgccgcgt
     2701 gaccagaagg cggttaaaac agcggcgcaa accatgcggc ccaatcctgg ccttgacctg
     2761 gaggcggcca tcaccgaact gggcgtcggt gaggcgttgg tgtcgctgct cgacgagaaa
     2821 gggcgtccgg gcgtgacgca gcgcgcctgg atgctggcgc cggcgagccg catcgggccc
     2881 gccaccgagg ccgagcgggc gatgctgcgc gccgactcgc cactgggggc caagtacgat
     2941 aaagccatcg accgcgagtc cgcctacgag acgttgtcag cgcgggttca aggagatccc
     3001 gagcccggag cgcaaggcgc gccggaatcc actgcggaat cgggcgacgg tggtgtgatg
     3061 ggcgcggtgg gtgaatttct gttcggatcg accgggccgc gtggcggcca ccgcgacgga
     3121 ctggtacagt ccactctcaa gagcgtggtg cggcgcgagg catcacaatt gttgcgcggc
     3181 gtactgggtt cgctgatgcg gcgccgttaa ggatccgccc gaacaagtcg actggaaagc
     3241 aggcgccgca gtctgttctg aagatcatca aggcgcatga atcctggctt ttagacattc
     3301 ctttcaaaaa tcgaagacag accgtgacag taaaagcaat cgtggccact cagtcggccg
     3361 acaacactcg ccgctggtgc caagcgtttc ggaacgcctg gcccgaggca cgattcgatg
     3421 cctggtcaga atcctccccc gtgccgtcgg gtgcccggta tgccattgtg tggcagccgc
     3481 catcggcgct gttccggaac gaaaccgctc tcgaggcggt gttcaatctg ggggcgggcg
     3541 ttgatagctt gttgcggttg cccgaactgc caaggtcgct gtccatcgtg cgcctcgaag
     3601 gggccggtat ggcggagcag atggtggagt acgtgcttta cgggctgttg cgggcggcac
     3661 gcgagttctc caactacgcg gtctcccagg cggatgtgcg ctggcagacc cgtcccgcca
     3721 tccgccgaga ggactggccg gtcggcgtca tgggtctggg cacgataggc accgaggtgg
     3781 cgcaagccgt ggcccgtctg ggctacccgg tggctggatg gtcgcgcacc gcgcggcccg
     3841 tgccgggcat tgaaattttt ggtggccagg agaatctggc ggctttcctg gctcgcacac
     3901 gtgtgctggt caatgtgctg ccgctcacgc cggacaccga ggacatcctg aaccgcgaca
     3961 atctgtcgcg ccttttgccg gatgggtatc tgatcaacgt ggcgcgcggc cggcatctgg
     4021 tcgaggaaga tttgttggcg ctgctcgatg ggggccgatt gatgggcgcg atgctagacg
     4081 tcttccgcac cgagccactg cctgcggatc acccgttctg gcgccacccc ggtattgtcg
     4141 tcacacccca tgttgcggcc atcacgctgc aggacgacac cgtcgcgcag atcgttggaa
     4201 agatccgcga catggagcgc ggcgtcgcgg tatcgggcgt cgtgaatcgc gggtcgggtt
     4261 attgattttc attcactgga gaaattttgt gtcgacgttg cataagatcg ccgtgatgcc
     4321 gggagacggc attggcaaag aggtggttcc cgaagggctg cgagtccttg aagccgcggc
     4381 gtcgcgcttc gacataggct tcgactggga tcacctggat tggggttgtg actactacgc
     4441 cgcgcatcag cgcatgatgc ccgacgactg gaaagagcgc attgggagcc acgaggccat
     4501 tttcttcggt gccataggct ggcccgacac cgtgcccgac catgtcgccc tgtgggggtc
     4561 gctgtttcgt ttccggcgcg agtttgacca gtacatcaac ctgcgcccgg tgcgtctgat
     4621 gccaggggtg acgtcaccgc tcgcgggccg cgcgcccgga gacatcgatt tcttcatcgt
     4681 gcgtgaaaac accgagggcg agtattccaa tagcggagga cggctgttcg aaggcacccc
     4741 gcgcgaggtg gtcatccagg aaagcgtctt cacccgggcg ggtgccgagc gggttctgaa
     4801 attcgctttc gaactggcgc gcaagcgcgg gcgccacctg acggccgcca ccaagtcaaa
     4861 cgggatttcg atctccatgc cctggtggga cgagcgtgtc gccgacatgg ccagccagta
     4921 tcccgacgtg cgctgggaca aataccacat tgacattctg tgcgcgcatt tcgtgcagca
     4981 ccccgactgg ttcgatgtgg tggtggcgtc caatctgttc ggcgacatcc tgtccgatct
     5041 ggggcccgcc tgcaccggca ccatcggcat cgccccctct gcgaacctca accccgagcg
     5101 gcggttcccc tcgttgttcg aacccgttca cggctcggcg cctgacatct acggcaaagg
     5161 catcgcgaac cccatcggtc agatctggag tggggcgttg atgctggact ttctcgggta
     5221 tcccgacgcc gcgcaagccg tggtgacggc catcgaggcg gtgctgatcg aagggccgcg
     5281 cacgcccgat atgaaaggca atgcctcgac gtccgacgtc ggcaaggcta tcgccgaacg
     5341 agtggcgcac gaatcctgac actttttctc caaggagact atcttgagtc gctatgtcaa
     5401 ggcgatccgt gtcgacgagc acggtggacc ggaagtattg aaactcgtct cggtcgaact
     5461 atccgcgcca caggatgacg aggtcacgat cgaccacaag gccatcgggc tgaacttcgt
     5521 cgacatctac tggcgcaccg gcctgtacgc gcgcccggtg ccccatggca tggggttcga
     5581 gggtgctggg gtggtgtccg ccgtggggcc caaggtccgc cacctcaagg tgggtgatcg
     5641 tgtcgcctac gggcaaagtc cgatcggtgc gtatgccgaa gcgcacaacg tgcccgagcg
     5701 gttggtggtc aaattgccga agtcggtcgg attcgacgag gccgccgcgc tcatgctcaa
     5761 aggcttgacg gtgcagtatc tgttccgcca gacctatcgg ctgcagggct acgaaaccat
     5821 cctgttccac gcggccgcgg gcggagtggg gctgatcgcc tgccaatggg ccaaggcctt
     5881 gggtgtcaaa ctcatcggca ccacatccag ccctgaaaaa gccgcgctgg ccaaggcggc
     5941 cggagcctgg gaagtcatcg actatacccg tgagaacgtc gtggagcggg tgctggaact
     6001 cactggcggc aagaaggtgc ccgtggtcta cgatagcgtg ggcaaggaca cctggatgac
     6061 gtcgctcgac tgcctgcagc cacgcggcct gatggtcagt ttcggcaatg cgtcaggccc
     6121 tgtcgacggc gtgaacctgg gcattctggc cagcaagggg tcgttgtacg tgacgcggcc
     6181 cactctgggc acacacgttc tcgcacgcga gcagttggtc gaggcggccg acgaactctt
     6241 cggcctggtg acgaagaaga aaatcgtcgt caatatcggc cagaggttcc cgttggcgcg
     6301 cgcggcggat gcacagatcg cgctggcggg acgcaaaacc acgggcgcga cggttcttac
     6361 tgtggattga cgctggttcg gcgggcgatg aaacgcgtgc ccgcccaggc gttccgcccc
     6421 tcgatcaggc gttctgcgtg gcctccgcgt ggtaggcccg cagacgctcc acttcgtttt
     6481 tcgaacccag cgcgacaccc acgcgctggt gcagcgcatg gggttgaatg tccagaatgc
     6541 gatcggcgcc gtcgatggcg cacccacccg cctgttccac aatgaaactc atggggttgg
     6601 cttcgtacat cagccgcagc ttgccgttgc gcgtggacgc ccgttgatcc ctcggataaa
     6661 tgaagattcc gccgcgagtc agcaggcgat ggacgtcggc caccatggac gcgatccagc
     6721 gcatgttgaa atccttgccg cgtgaccctt ccttcccggc caggcattcg tcgatgtagc
     6781 gtcgtaccgg cgcgtgccaa tggcgcatgt tcgacatgtt gatggcgaac tcggccgtgt
     6841 cttcgggaat ggtgaggttg gcctcggtga gcacccacga gcccatttcg cggtccagcg
     6901 tgaaggccac gactccggtg ccgactgtca gtatcagcat gctctggggg ccatagatgg
     6961 cgtagccggc cgccacttgg cggctgcccg gctgcaggaa atcggactcg tccacgtcgc
     7021 gcccggacgc gtggtgcggg gcgtgcagca ccgagaagat ggtgccgatc gacacattca
     7081 cgtcgatgtt ggacgaaccg tcgagggggt cgaaaagcag caggtattcg cctttgggat
     7141 agcggttcgg gatgcgatgg atggtttcca tttcctccga ggccatggcc gcgaggtggc
     7201 cgccccattc gttggcttcg agcaggattt cgttggacag gacgtccagc ttcttctgga
     7261 cttcgccttg gacattctcg gtgtccaggc ttcccagcac gccgcccagc gcccctttgc
     7321 ccacggcatg gccgatggct ttgcatgctc gggcgaccgt ttcgatcagg agccgaacct
     7381 cggctgatac cgcctgtttc tgtcgttgct gttcgaccag gtattgggtg agtgtgatgc
     7441 gcttcaagtg ggatccccct tccttccgtg atgagccgat gtgtcgagac tacaggctga
     7501 gtgatttcgt gatgatttcg ctgacgttgc gcgacagggc aggctgggcc tggatatggc
     7561 gcagagcctg ctccatgctg tcgcggtgtg gctgtgcgaa gcgggtccag ttgtcgaagg
     7621 cgcgcgccag tcgggcggcg acctctgggt tgaaccgatc gatggcgatg acttgttctg
     7681 cccagaatgg gtagccttcc ggcgcatgga tggcgcgcag attgttcatg cagaactgaa
     7741 agagcaggga gcgtgcccga ttcggattgc gcagcgtgaa cgcgggatga agcatcaagg
     7801 cgcgtaccgt ttccacgcgg gtggaggggg ccgtggcctg cagggcgaac catttgtcga
     7861 tgaccagcgg gtcgtgctgc cattggtcat agaagtgctc cagggccggg ccggcgtcct
     7921 cgacgtcacc gaagttgacc agggcggaca gaccacccat gcgatccgtc atgttgcggg
     7981 cgccaaaata ttggtcccgt gcctgcctcg cgccgtcggc atggccaccg gccatcaggt
     8041 agacgagcgc caggttcttc aaggcgcgtc gccccgccga gaccgtgtcg atgcggtaat
     8101 tgtcatcgtg gtcggtgtgg cgcgaatagg catccagcca caaaggggcc agtgacgcgc
     8161 ccagcacctg gcgcagatga tcgcgcgcct gcgtgacggc cagcggatcc atgggcgccg
     8221 ttttttccag gatgattttc tcgggaggca gggagagcgc ccgtgtgcga taggccggcg
     8281 tcagggattc gtcctggagc aatgtgcgcc aagtgtcgat gaaagcttgg tcaacactga
     8341 gggtcgagcc tgactgcccg gcgcgcacca gcgccagcaa ttgtcgggtg gccagctcct
     8401 ggcacgcttc ccagcgcgcg aaggggtcgg tatcgtgccg ggccagcagg gccaggtcgg
     8461 cgtccggcct gtcgtaatca acgatcacgg gcgccgagaa attgcgcagc agcgagggga
     8521 tgggttgttc gggaataccg gtgagcgtcc aggttcggct ggtttcagta agttccagca
     8581 gaacggtatc ggtcacccgt ccgtcgtggc cgatcggcag aggttggcca ttggcgccca
     8641 gcagccccag ggcgaatggg atatgcagag gttgcttgtc caatggtggg ttggcgagct
     8701 tttcgacccc gaccggggcg caatgctggc tgagcgtgac ctcgcacgtt tgcgagccct
     8761 tgtcatggcg caatgcgacc gtgactcgtg gtgtgcccgc ctgcgaatac cagcgccgga
     8821 agacatccag attcttgccc gggttctgcc gcacatagac cgattccatg gcgtcgacga
     8881 agtcgtcgca ggtgacggcg ttgccgtcat gtcggcggaa gtattcgtcc atgccggccc
     8941 ggaaccccgt ttcgcccagc aaggtgtgct gcatgcggat gacttccgcc cctttttcgt
     9001 agacggtggc cgtgtagaag ttggcgatct cctggtagct ttcggggcgc accgggtgtg
     9061 ccatcggccc ggcgtcctcg gggaactggg ccagccgcag catgctgacg tcgtcaatgc
     9121 gtttgacggc gcgcgcgctc agggcctgga tatcgtccag gccctgagcc atcatgtcgg
     9181 ccgagaattc ctgctcgcga aacacggtca acccttcctt cagcgacaac tggaaccagt
     9241 cgcggcacgt gacgcggtcg ccggtccagt tgtggaagta ttcgtgtccg atcaccgact
     9301 cgatgctgcg atagctggcg tcggtggcgg tgtccgggtc ggccagcaca taggccgaat
     9361 tgaagatgtt gagacccttg ttttccatgg cccccatgtt gaagtcgcgc gcggcgacca
     9421 ccatgaaacg gtccaggtcg agttcgaggc cgaagcggtg ttcgtcccat tccattgcat
     9481 gttgcagcga gtccagcgcc cagccggtct tgtcttccga gccgcgatcg ctgtagatct
     9541 gaagcagggc gtcgcgctgg ccgcgggtct tgacagtgcg ttcacgcacg ctgaagtcgc
     9601 ccgcgaccag cgcgaacagg taacagggct ttggatgggg atcttcccat atggcttcgt
     9661 gtcggccttc gggcagttcg cgggcctgga tcaggttgcc gttggagagc agcaaaggga
     9721 agcgcgcctt gtcggcttcc agcgtcacgc ggtaggtggc catgacgtcg ggcctgtcgg
     9781 gaaaccaggt gatgcgccgg aaaccctggg cctcgcattg cgtgaacaga ctcgcgcccg
     9841 acacatacag ccccatcagg gatgcgttgt cggcaggccg gcacaggctg atgatctcga
     9901 gctcgaatgt atcggaggcg ggttgcaggg tcaggtgctc atcatcgagc tgatactggg
     9961 cggggccaag cggttggcca tcgagtttga tggattccag cgtcaggtca cgcccgtcga
    10021 gaaccaatgg cgcatccacc gggccgtgga acctgagtgt ggcgcggacc cgggtttcgg
    10081 cgggatcgag ggtgaagtgc agcgccacat ggtccagcgt gtgtgaatac ggccggtagt
    10141 tgtggcgcga aatggttttt cctgtttggt ctggcatgtt ggcgactcgt tcagattgaa
    10201 ttcatattgt agagtagtga cttgtgcagg gggggctctg cgaggtgtgc cccggtctgt
    10261 tggcaatagg tgggaccggt tctcttgaaa gggagttcct tccacgttga actgctcacg
    10321 gttcgtgcca tactgtttgg agcgaacggt tcatgggttt tgtgtcgggt gcaaatcatg
    10381 tcgtttttca tgtctaccgg gtatcaaggg cggtcgggcg cgttgcgcaa gctggcgcgc
    10441 gcgagcatta tcctggtcgc cgcgtgcttt ctggccggtt gcgccaccac gctatcggca
    10501 cgcgtcacgc ggttcgagaa atggccggcg aatgcggttg gcgccacgta ccgtctggta
    10561 ccgggggcgg ggcagtccaa taatcttgaa tatcaggctt ttgccgacac gatgcgggcg
    10621 gccattgggc cgactggctt ggtcgaggcg cgaagcggcg cgttggcgcg gttcgacgtg
    10681 tcgttttcgt atgtggcgac caaaggtgtt gaatggatga cgcgctatgc cgatttcccg
    10741 ccttatgccc cgtgggggcc ttgggggtat tacggcggag gcccgttcgg cccgccgatg
    10801 gtcaatgtgc ccgtcgccgt atatcggaac gaactgatcg tcattatcaa ggatcgtagc
    10861 cagaacgatg ccgaagtcta ccgttccagc gctgtcacaa atacggcgag caaggatctg
    10921 ccgcaggtga tgccctatct ggctcgcgcc gttttcgacc actttccggg caacaacgga
    10981 caggtgatgg tcgtgcgtta cacattagga gggcgttgac gaatgcctcc gtcgcgttgt
    11041 gaatcgcagc gatagcaacg actatcgctg cgattggcac cttgcgttca acctcgatcc
    11101 acagcagccg ttctttgttc gactcggtcg aagggcaccc ggacgcccac gctggccgcg
    11161 atggattgtg gttctggatt attgttcaat cagaaacgcg tcgcgttgct tgccgtctga
    11221 ttcggccttg atgatccagc gcggcgcttt tccgcgcccc gtccaggttt cgcctgtttc
    11281 ggggtggcga tatttgatcg gcaccgaacg cttggcggcg gatgccgccc cattggcctt
    11341 tggtgatgcc actttgacgg ctttcttgtt cagcgcctgg gcgatttcct ctggtgtgat
    11401 gtcgtattca cgcatggtgc ggacgataga ggcgatgact ggaacacgtc ttttgatgcg
    11461 caaggcgctg gcttgtttct ggagttttgt gatttctttt tcaattttcg cctgtagagc
    11521 ggaatacgta tcacgggaca taatcggttc ctgttgtaag tggtatgtgg gacttctatt
    11581 atggggaaaa gcctctcatt ttatgaatga gagggtgagc tgatttaaga tctgtgtaag
    11641 caaggtgtgc ggatatgcct agtggcagcc taggggagtt tttcgaaaat caatcgtggc
    11701 cattcaataa tccacctcca atccacggcg ccggcctgtc gttcgagttg ttcagagggc
    11761 ccattgccgg tttgcagatg cgtccccatt ttgattttcg tgaaaatccc tgtaccgtga
    11821 attatatcaa cgagaaaata atttttgcct gacagataat aatcaatgtc gcgaatccgg
    11881 cacaaattca tcctgtcccg gtatgtcctt gaaggttatc attttcttgt ttttttaaac
    11941 gtttgaatgc ggtcaaccaa catgaattca aattccattc ccgacgattt tcgtgtggcg
    12001 gcggtacaga ctgtttcatc cacctcggtg gatggcaatt tgaaaatggc ggctgatctc
    12061 atcggccaag cggccgaaac cggcgccagg cttgtcgtgc tgccagaata tttctgcctg
    12121 atgggtcatc gcgatggcga caaattcgat attcgcgaaa aaccgggcgc cggacccatc
    12181 caggaattcc tgcgttctca ggcccggcat catggtatat ggctggtggg ggggacgttg
    12241 ccgctggaaa gtcccgatgc cggccgcatc tataattcaa gcttggtgtt ctctcccgat
    12301 ggcgcgcagg tggcccgcta tgacaagatc cacctgtttt cctttcatcg tggcgatgaa
    12361 tcctacgacg aatccgtggc cattcggccc ggcgataatc cgccgcaggt gttttccgcg
    12421 tcctgcggta gggtgggttt gtcgatttgt tacgacctgc ggttccccga gtttttccgt
    12481 gcattgggcg acctgggcct gatcgtcatg cccgcggcgt tcacctatac gacaggaaag
    12541 gcacactggg agattctgct gcgtgcccgg gccatcgaga accagtgcta tgtgctggcg
    12601 agcgcgcaag gcggcaagca tgagaatggg cggcgcacat gggggcattc ggttctgatc
    12661 gatccctggg gcgaaatcgt cgacgttctg ccagaaggcc ctggtgtggt gtcgggcatg
    12721 atcgacgccg gacgcctgtc cgatgttcgc caatcgcttc ccgcgctgaa acatcgtacg
    12781 atgtagccta ggggttctcc gaacaagtcg agcggaaggc gcgcatccat gcatcgttgc
    12841 ggtggcttgt tcagaggggc caccgtgtct gtgcttccat ccaatttttt attgaagata
    12901 tcgtttcatg aaagtctccg atcccgcgat cagcgccctg gctacggcga aatcggttct
    12961 gctcgaccct tgggggttga gcgaagcgga tatcgcccga gccctgggtg aaatcttcac
    13021 gcataaagtc gattacgccg acctgtattt tcaatatacc cgcgccgagg gctggagcct
    13081 ggaagaaggc atcgtcaaga ccgggaattt ctccatttcc caaggtgttg gcgtccgagc
    13141 ggtcagcggc gacaaaacgg cgttcgccta ctccgatgcg ctgtccgccg atgcgctgct
    13201 gtcctcggcg cgcgccgttc gcagcattgc gcgccagggg gcggggcggc gcaaagtggc
    13261 gcgccctggc aacggccgtc ctcatgatct ttaccgcgat ctcgaccctg tcgattccct
    13321 gtccgctccc gacaaagtgg cgttgctggg gcgggtcgag gccatggcgc gggccgctga
    13381 tccccgcatc atccaggtga tggcggggct gggtgctgaa tacgatgtgg tgctggtcgc
    13441 cggtagcgac ggacgtctgg cggccgatgt caggccgttg gtcagaatgt cactgaccgt
    13501 gatcgcggaa caaggcgggc gccgcgaaat ggggcatggc gggggcggtg ggcgcaccgg
    13561 gctggcctgc ttcaccgacg aggccttgcg cggctacgtt cgccaggcgg tgcacgaggc
    13621 cctcaccaat ctcgaggccc gccccgcgcc cgccggcgaa atgaccgtgg tgctgggctc
    13681 ggggtggccg ggcatcttgc tgcacgaggc cgtgggtcat ggcctggagg gcgacttcaa
    13741 ccgcaagggg tccagcgttt tctcgggccg cattggtgaa caggtggcct ccaagggggt
    13801 cacggtggtc gatgacggca cactggccga gcgccgcggc tcgctcaaca tcgatgacga
    13861 gggcaatccc acgcagtgca acgtgctgat cgaagacggt gttctcaagg gttatatgca
    13921 ggattccctg aatgcccgcc tgatgaaaac cgccgtgacc ggcaatggcc ggcgcgagtc
    13981 gttcgcgcac ctgcccatgc cgcgcatgac caacacctat atgctggctg gatccgaggc
    14041 cccggaggac atcatcgcgt cggtggacaa tgggttgtat gcggccaact tcggtggtgg
    14101 tcaagtcgac atcaccagcg gcaagtttgt gttctccgcc tcagaggcct atctgatcaa
    14161 aaacggccgg gtcacacatc cggtcaaggg ggcgacgctt attggaaacg ggcctgacgc
    14221 catgacgcgc gtgcgcatga taggtaacga cttgcgtctg gactccggca ttggcacctg
    14281 cggcaaggag ggccagagtg tcccggttgg ggtgggcatg cccacactgc gcatcgacgg
    14341 tctgacggtg ggcggcacgt cctgatattt tcgaggcggc cccgcttccg tcctccttat
    14401 gcggtagtgc ccacgcccgg gtcgtcgttt cccaggcgtt ttggtctctc cattcaagcc
    14461 gccgcgcatt cgccggctgc tcgccgtttc ctggccccac tgtcactgtt cgtgtctcca
    14521 tatttctatg gcgcatagag ggtttcgtgt gctcttcctg gtcgaaggcg caggaaacgg
    14581 attgccgtga atgcctgttc ttttttcgtc tgatttgatt gacatgaatg attattaatc
    14641 atatgatttc aactcaaatt aattcatgtg aattattttc tatcgtgacc aattcatcac
    14701 aaaacacaca acagtacgac ttgcgcatcc tgcgcgcgct gcggcgcctc acccgctcca
    14761 tcgcgctgca ttcgcgccag cttgcggcat gcagccacat cacggcgccg caactcatct
    14821 gcttgcgcgc ggtcatcgat catggcccgc tcaccgccac ggcgatcagc cgcgaggtgc
    14881 atgtcagcgc cagcaccgtg gtgggtattc tcgaccgcct cgaagacaag gggctggtgc
    14941 gtcgcgagcg tggccgtgaa gaccgccgca tcgtgttcgt gtcggccact gaacaaggcg
    15001 ttgccttggc gcgtgaaaca ccttcgccgc tgcagaaaaa gctggccgat gcgctcaacg
    15061 cgctgcccga actcgagcaa gccaccatta cgctgtcact ggagcgtgtg gtggaactga
    15121 tcgagtcggg cgctcccgaa ttgcgcgatg tgcccggcga tgcctcgcca ctgcttgatg
    15181 tgcccactga tggcgcgctg ccagaatcgg ggctggtggt ttgacggatc ccgacgttat
    15241 ggatgttgtt tgcgaacagg aaactctcga tgacacaacg caggtcgccg aagcacccct
    15301 gatactgcgt gcgccgggca agcatgatgg gcgggccatc cacgacctgg ttgcccactg
    15361 tgcgcccctc gatctgaact cggtctacac ctatcttctg ctggccgagc atttttcacc
    15421 cacttgtgtg ctggctcatg cacaagatcg cctgcgcggt tttgtatcgg cgtatgtgcc
    15481 gccaggccgg ccggacgtgc tgttcgtctg gcaggtcgcc gtgcatcggc aagtccgggg
    15541 caggggtgtg gggccgcgca tgctcaggca tctgctcgaa cgccccggcc tggaagacgt
    15601 ccgctatttg gaaacgactg tggggccctc caatcgggcg tcccgcagca tgttcgcccg
    15661 cgttgccggc agccttgggg cttcggtgcg tgaatcccgg ctgttttccc gcagcctgtt
    15721 cggcccgcat gggcacgacg acgaacccct gttgcgcatc ggcccgttcg atgcgcactg
    15781 aaagaggaaa agcgatggat ctgaaaatat tcgaacggat ggagtccgag gtcaggggct
    15841 atatccgatc cttcccggta gtgttcaccc aggctcgcgg ttcgctgctg gtcgacgaag
    15901 acaagaatga atatgcggac ttcttcagcg gagccggcac tctcaattac ggccacaaca
    15961 atccgttgct gaagcggcgg ttgctggagt atctggaaac ggacggtgtc gtgcacgggc
    16021 tggacatggc cacgagtgcc aagaagtatt tcctggagac tttcgagcgc acgcttctcg
    16081 ctccacgcgg ctggtcgtac aagcttcagt tcacgggtcc aacgggcaca aatgccgtcg
    16141 aagcggccct gaagctggcc cgccaggtca aggggcggcc caatatcatt tcattcaccc
    16201 atggcttcca tggcgtgagc ggcggctcac tgtcggtgac ggccaactcc aagtttcggc
    16261 aggccgcggg gttctccctg gccaatacga cgttcatgcc ctatgacggt tatctcggcc
    16321 ccgatgtgga taccatgacg tatctcgaac gcatgctgga cgatcccagc agcggtcttg
    16381 atcatcccgc ggcggtcatt gtcgagaccg tccagggcga gggcggggtg aacgtggcga
    16441 gctggcgctg gctcaaagat ctggagcgtc tgtgccggcg gcacgacatg ctgctcatcg
    16501 tggacgatat ccaggtcgga tgcggccgca cgggcaattt cttcagcttc gaagcggccg
    16561 gcatacagcc cgacatcatt actttgtcca agtcgttgtc tggcttcggc ctgcctatgt
    16621 cgctggtgct gttccgccct gagcttgaca tctggaaacc cggcgcgcat agcggcacgt
    16681 tccggggcaa taacctggcc tttgtcactg cggcgcaggc gctcgagagc tactggtccg
    16741 acaccaccct ggccaatgac acggcccgca aggagcgcct ggtgcgggac tggctggaaa
    16801 acctggctca cagttaccgg ggcgccgggc tgagcgtccg gggcaggggg ctcatccagg
    16861 ggctggttac cccccggtcg ggcgatgtgg ccaatcgggt ggctcacgaa gccttcgagc
    16921 gtggcgtggt aatcgagaca tcgggcgccc acgatgaggt gctgaagctg ttgcccgcgc
    16981 tcaccatgga cgatgatttg ctgcgccggg ggttggaggt gattgagagt gccgtgggca
    17041 gcgtgatgag gcagtccccg accaacgtgc gcgtacttga agcaggagga aaaaaccgat
    17101 gattgtgaag aacgtgaatg acgtgatcgg gacccgggac gaggtcaaga cggagacctg
    17161 gctcagccgc cgtgtattgc tgaagcagga cggcatgggg ttttcgtttc acgaaacagt
    17221 cattttcccc ggtaccgaaa cccacattca ttattgcaat cacctggaag ccgtatggtg
    17281 cattgagggc gatggcgaaa tcgaaaccgt tgcggacaga cgcaaataca cactggggcc
    17341 gggcgtggtg tatgcgctgg acgaacacga cgaacattgg ctgcgcgggg gaacccggcc
    17401 gctgcgtgtc atctgcgtgt tcaatccgcc gctcactggc caggaagtac atgacagcaa
    17461 cggggtctac cctgcggcac aaccggcttc cgcctgacag gaggtcagat catgaataca
    17521 cctgtaagcg acgtttaccc atcacgctcg gatcgggacg cggccattat tgcgcgacag
    17581 gatccggtcg tctatggcat gggctcgtac gcggaagcgc tggcgccgga acggcttgcc
    17641 gcctacgagc gcgacggctt tctgctggtc ccggacctgt ttccgcatga ggaggttcaa
    17701 gccctgtcgg ccgagatcga gcgcatgaag gctgatccgg ccatggccgc tttggatgaa
    17761 accattgccg aaccgcgcag ccaggtgatt cggtcgattt tccgggttca tgagctgagc
    17821 gacatcatgg ccggactggt gcgcgacccc cgtgtggccg acgtggcgcg ccagatacta
    17881 ggttccgagg tctacgtgca ccagtcacgg gtgaatctga aaccggggtt cgagggcagg
    17941 gaattctact ggcactccga tttcgaaacc tggcacgtcg aggacgggat gccgcgcatg
    18001 cgcgcgctga gctgctcgat cctgctgacg gacaacaccg cgtgcaacgg gccgctcatg
    18061 ctcatgccag ggtcgcaccg gcagttcgtc tcgtgtcgcg gacgcacgcc cgatgaacac
    18121 tacaggatgt cactcagaaa gcaggagtat ggtgttcctg accagatcag cctgaggttg
    18181 ctggcggaac agggcggggt cgaggccatg acggcgggag ccggttccgt cgtgtttttt
    18241 gactgcaaca ccatgcatgg gtcgtcgggc aatatttctc cgctatcgcg cgccaatgtg
    18301 ttcgtggtgt acaacagcgt cgacaacgcc ctgcgcgagc cccgccacgg gctcaaaccc
    18361 cggcccgaat atatcgcggc acgccagtcg tgtgatccgg tgcgctcaca cgaggcagcg
    18421 tctgcggcgt agcatccagc ctcctgtgcg aggccatttc catagagcga tggcgcttgg
    18481 ccaatcctga agcagccgct tccgccgggg cgtggcggct ttccgcgggt caggcccgtt
    18541 gtatgtagcc cttcagaaat tcctgggtcc gggcttcccg gggtgacacc atgatctcct
    18601 gcggcgggcc ttcctcaacg atccggcctt tatcgaagaa acagacccgg tcggagatct
    18661 gcttggcgaa ttgcatctcg tgcgtgacca gtagcatggt caggtcgtgt tcgccggcga
    18721 gacgctggat gacgttgagc acttcaccca ccagttccgg gtcgagcgcc gaggtgggct
    18781 catcaaacag catgatgttg gggtgcatgg caagcgcgcg cgcgatggct acgcgttgct
    18841 gctggccacc cgaaagctgg ctcgggaact tgttggcctg atccgacagg ccgaccaagt
    18901 cgaggtattt ctccgcccgg cgggcggctt tttccttgga cagcttcaac acgtgcatcg
    18961 gggcttcggt gacgttgcgc cgcacggtca tgtggggaaa caaattgaac tgctggaaca
    19021 ccatgcccat ttccttgcgc atcgtccgca gatgcgcctc actggccggc accagttggt
    19081 tgttcttttc ctcgtgccac agaggcttgc cgtcgacata gatgaagcct tcgttgatgg
    19141 tttccagcgt catgaggatg cgcagcaccg ttgatttgcc cgacccggag gggccgatga
    19201 tggtgacctt ctcgcccttg cgcacgttga aattcaggga gtcgaggatg gtgacgctgc
    19261 cgaaacgctt gacaacgttg tcgaacttga tgatgggggc gtcgtcgttc atcgcagggg
    19321 cagtccttgc ttgggtagat gggtatccag gaagcgcaca ccggcggacg ccacgagggt
    19381 cagcaccatg tacaggccac ccaccatgga cagaggcacc atgtagtcga aggtccggtc
    19441 gccgatgatc ttggccacat tgagcatttc gagcaccgac acgaccgata gcaccggtac
    19501 atccttcatg atggagacca gatagttgcc cagggccgga atgatgcggg gaatggcctg
    19561 cggcaggaca atgatggtga atgtgcgcag ggcggtgaag tccagggccc gtgcggcctc
    19621 agtctggcca tgagccaccg attcgaggcc ggcacggtag acctccgaca tataggcgct
    19681 gtattgcaca cccagggcca gcgcgcccgt caggaaggca ggcagcacta ttccgtagtc
    19741 cggcagcacg taatacagga agaaaagctg gataagcagc ggcgtgtcgc gcaggaattc
    19801 cgtgatcagc cgggcaggcc aggaaataaa gcgtgagcgt gcgcttttga gcgcggccag
    19861 gatcaggccc agaatcgcgg ccaccagaaa gcctacgatg gtcgcttcga tcgtgacgac
    19921 cagtccacgc agcagaatgg ggaagatgga gctggcaagt gtccaactat tggtggtgtc
    19981 ccagtgaatg ccgaacagca tgtcaggatc tccccctgcg ccagcgcccg actgaccgct
    20041 cgagcagttg catgatggcg gtcagaacca gcgccatgcc gaaatacatg aacagcagca
    20101 aggtgtagac cgtggtgctg tcctgcgtga acgtgcgtat ctgttcagca cggaacgcca
    20161 ggtcgcccag gctgatgagt gataccagag ccgtgtcctt caggttctgc accgccaggt
    20221 tgccgaaagt cggcatcatc tccggaatcg cctgaggaat ggaaatgcgc caaagcgtct
    20281 ggcgcggagt aaagctcagc gcgcgtgccg cctcgtgctg gttgggatgc accgcctgta
    20341 tggcgccccg cacaacctct gcgccgtagg ccccgatgtt caggctcagg gccagggtgc
    20401 ccgcaatgac tggcggcagg cgcagatcca ggccgatcgc ctgcccgaag atcggcagcg
    20461 cgaaatacag ccagaacagt tggaccagca gcgacgtccc gcgaaagacc tcgatgaaga
    20521 atatcgagac acccttggca gcccagttcc tggagagttt ccccacgcca aaggcgaagg
    20581 agaagatgcc acccagcacc gtcgagtaga acgtcagttg tatggtcacc caggcgccct
    20641 gaagcaaggg agggctgtac atagtccaat tcatccgccg ctactcctgg gtcagacgtc
    20701 gcttgaaacc gctcgcgcgg acgaggttgg cgcccccggg gcgttgagag gaaatggcgt
    20761 gccaccagcc gtcatgacgc atgacagccc cgtttccggg gaaacggggt ggcggggcac
    20821 agctccaccc acctgccgcc ctccgggaag ggccaggctg ggcgaccggt ggcggtgtgg
    20881 tcagccgcca gagcacaact tactggtggt cttctcgaaa gaggctttgt tgtcggcctc
    20941 ggtgaacccg tactgcgtca tgatcttgct ccacgcaggc gttttcttga acttggccag
    21001 ttgcttgtta acggcgtcac gcagccccgt cgacttcttg gaaaagacga acgcgcccca
    21061 actgcgcacg ggcttgccgt cgacgaccgg gtctttgaag ttcttcgcaa cctctacttt
    21121 ctgactcttg gcggccaatt ggctggccgt caggctggtg gcggcgtagg ctgctgcccg
    21181 gcccgtggca acggtggaga tagcgtcgga attgctggag atcgtgacca tctggccagc
    21241 cggcactttc aaggcctgca gcatttcaag ctggtcggcg cccgccatga tggcgatttt
    21301 cttgccgttc ttggcgaatg cttcgtagga gtggaggttg tccggattgc ctttggcgac
    21361 tagaagccct tcgccatagg agctgtccgg ctccgagaaa atgaccttct tgcagcgggg
    21421 gggcagtacc gccatttcgg cggcgaccat gtcgaagcgg ttggcgagca ggcccggaat
    21481 gagtgagccg aagttggtgg cgacccactg gatttctttg acgcccaact gtttcatgac
    21541 ctgtgttgcg acatcggggc cggccccttt ggcattgcca ctggggtcta tgtagccgta
    21601 ggggatctca ttggcgacag cgatgcggat atagccgcgg tctttgatct gtttcagggt
    21661 ttccgcattc gtctggttgg cgatgcccag ggccagactg gcggccgaaa gggccaataa
    21721 tgcggttcgg tacgtttttt tcatgagcgt atcctttgtc tctgattatc gggaccggga
    21781 acggtcggaa tgcctgattt ttgttatgcg ttcccactgt ggtggggtgg cagtcattaa
    21841 aatcatacac tagggtttat tttcaattca aattcagaac catcaggaat tcggataatt
    21901 cgacagaata ttttggtggt gtcgagtgcc tgcggatcaa gcctatttat cgagcccggg
    21961 catcggctgt atccgtggcc tatgggggga gccttcacag atggagccgg cgttcgcgca
    22021 ttgttccaac aacctggaat caattgatct ggaacgtcac tggaaatttt tgggtggcgg
    22081 ccatcgcaaa cacagaaaat ttggtgcggc ctcaccgcca atacagaaaa atcgtgggcg
    22141 atttattttg ctggacactt ttgtttgatg gggccgatgt gcgtcgtttc cctcctgggt
    22201 tggtcaggta ttggcccagg gtgccgtgga taatataggc atgattctcg ccgcgctcag
    22261 cgtggcggta ttgtattttc aaactggaat atttttaatg ggaaacccca atagatgggt
    22321 tgggaaaacc actatattcg gctgggggat gaggaattca atacaatata ttgagcgttg
    22381 tttgggagat catgcgcttg taggggggaa gagacacggc tgaccgggga atccccggcg
    22441 cccggaattc atttggctga aattttcgct ttacgtcacg ctttgccggt gctaccatct
    22501 cctattatgc gtgccgttgc ttcgtatttt ttctattttt actttacttt tccgaaaccg
    22561 ctggtggagg aagggaagcg tcgcacgcag taagcaatca accaagttgc cgaattcccc
    22621 gaaaagccgc cagcgaagct aggcggcttt tttcatgccg cggcgatgct gacattcact
    22681 ctggagccgt tccgtgtcac acaataccga cgatttacgt atccgtgaaa tcaaagaact
    22741 cagccctccg gcccatgtca tgcgtgaatt cgtgtgcacc cccgaaatat cggccaccgt
    22801 ctatggggcg cgacgcggtc tgcaccagat acttcacggt gaagacgatc gcttggccgt
    22861 ggtgatcggg ccttgttcga ttcatgaccc gaggggggcg ctggagtatg ccggtcgcct
    22921 ggccgagcag cgtcggcgct tcgccggcga gctggaaatc atcatgcggg tgtatttcga
    22981 gaagcctcgc accaccgtgg gctggaaggg cctgatcaac gatccggggc tggatggcag
    23041 cttcaacatc aaccaggggc tgcgcaccgc tcgcgaactg ctcatagaca tcaacggcat
    23101 gggactgccc gccggcaccg agttcctgga catgatcacg ccgcagtaca tcgccgatct
    23161 ggtgtcgtgg ggggccatcg gtgcgcgcac cacggaaagc caggttcatc gggaactggc
    23221 gtcagggctg tcgtgtccgg tgggtttcaa gaatggcacg gacggcaaca tcaagatcgc
    23281 ggtcgacgcc atcaacgccg cctcccagcc ccaccatttc ctgtcggtga ccaagggcgg
    23341 gcattcggcc atcgtctcga ctctgggcaa caacgattgc cacatcatcc tgcgcggcgg
    23401 caagcaaccc aactacgacg ccgccagcgt cgaggccgcc tgccagtttc tcgtcaagtc
    23461 gggcctgcgc acccagctca tgattgacgc cagtcacgcc aatagcaata aagactaccg
    23521 caaccagccg tccgtcatcg acgcggtggc ggctcaggtc gagggcggcg acagtcggat
    23581 ctcgggcttg atggtggaaa gccatcttgt tggcgggcgc caggatctgg tgcccgggcg
    23641 tgcgccggtg tacggccaga gcatcaccga tggctgcgtg gattgggaca cgtcggtccg
    23701 tatcctggaa cgcttggcca aggctgtcga ggcgaggcgg cgcgtggcga aagtgggggc
    23761 cgttgaggat tcgcccgccg cggcgtgaaa agggcggagg cgcttggcgg gaatggggca
    23821 ggatgcctac aatacgagta gtatcgggtg tctggaataa cgaaaaagat gcccgtgcca
    23881 aacagcgcgc caggcacctc aaggagattc cccatgaacg ccccgatggc cggccaggaa
    23941 gtacgccggc cgattcccga tggctttttc gattcgttcg aacagaagtt cggctcgcgg
    24001 ttttcccgcg cccgggcggt ccgcgagcat catggccgcg acgaatcccc gtatccgccc
    24061 atgctgccgg acgccgtcgt ctacgcgtcg accaccgacg aggtcgcctg gctggcgcgc
    24121 cattgcaacc agcacggcgt gccgctcatc gcctacggcg ttggctcgtc cctggaaggc
    24181 cacttgctgg cggtgcaggg cggagtcagc gtcgatttgt cgggcatgaa tcagatcgta
    24241 tccatcgatg ccgaagacct gaccgccacc gtgcaggcgg gcgtcacgcg caagcaactg
    24301 aacgaagcgc tgcgcgacac cggcttgttc ttccccatcg atcccggtgc tgacgccagt
    24361 ctgggcggca tgtcggcgac gcgcgcctcg ggcacgaatg ccgtgcgcta cggcaccatg
    24421 cgcgagaacg tcatgaatct caaggtcgtc accgccgatg ggcgtgtcat cgagacggcc
    24481 aaccgcgcgc gcaagtcctc ggcgggctac gacctcacgc acattttcgt tggcagcgaa
    24541 ggcacgctgg gcctcatcac cgaggtcacg gtgcgcctgt atccgcaacc cgaggccatc
    24601 tcggcggcca tctgcaattt ccccacactg gactcggcgg tgcaaagcgt catcgagatc
    24661 atccagatgg gtattccggt ggcgcgggtc gaattcatgg acacggccgc ggtcagggcc
    24721 accaacgcct acagcaaact cacattgcgc gagtcgcctc tactgttgtt cgaattccac
    24781 ggcagcgcgg cggctgtcaa agagcaggcc gaggctgtgc agggcatcac ccacgacaac
    24841 ggcggcatgg atttcgaatg ggccgaacac cccgaagacc gcagccgcct ctggactgcg
    24901 cggcacaacg cctacttcgc cggactgggg ttgcgtcccg gttgccggtc cagcaccacc
    24961 gatgtctgtg tgcccatttc ccgtctggcg caatgcgtca cggaaaccgc cgctgaactc
    25021 gaccgggcca gcttccccta taccatcgtg gggcacgtgg gcgacggaaa tttccatgtc
    25081 ctgatgctgc tcgaccctga cagttcggcc gagtgggacg aatccgaacg tataaacaac
    25141 agcctggttc gccgcgccat cgccatggac ggcaccagta cgggagaaca tggtgtgggc
    25201 ctgcacaaaa tggaattcat gctggccgag catggccagg atgcgctcga cctgatgcga
    25261 agcttgaagc aggctttcga tcccaacaat atcctgaacc cgggcaagat cattccgccc
    25321 cgggcgggcg ctcgatgagc gccggcgcag cggccgtggc ggacgacaac acaccggatt
    25381 tcgccggcct catggcccgg ctgggccagg ccatcccgtc ccattgcatt ctgtcgcggg
    25441 tcgaagacac ccggccttac gagtgcgatg gcctgtcctt gtatcgggcc ttgccgccgg
    25501 tggtcgttct tcccgagaac gaggcgcaaa tcattgaagt gctcaatgcc tgccgccagt
    25561 tccaggtccc catcgtggcg cgcggggcgg gcacgggcct gtcggggggc gccatgcccc
    25621 atgcgcgcgg cattttgttg ggcacggcca agctcaaccg catacttcgc atcgacacat
    25681 cgtcggcaac ggccgtggtg cagcccgggg tgcgaaatct ggccatctcc gaggccgccg
    25741 cgccccatgg cttgtattac gcgcccgatc cgtccagcca gatcgcctgt tccatcggcg
    25801 gcaatatcgc cgagaactcc ggtggcgtgc attgcctgaa gtacgggctc accgtacaca
    25861 acgtcctgcg cgtgcgcatg gtcaccatcg agggtgaggt ggttgagctc ggctccgagg
    25921 cccccgacag cccgggtctc gatctcctgg ccgcgttcat cgggtccgag ggcatgctgg
    25981 gggtggtgac cgaggccacc gtcaaactca ttcccaaacc ggcttgcgcg caggtcatca
    26041 tggccagctt ctccagcgtc gaggccgccg gccaggcggt gaccgacatc atcgccgcgg
    26101 gcatcattcc agccggcctc gaaatgatgg atcagcgcgc cgtccacatg gtcgagccgt
    26161 tcgtgaaggc cgggtacgac ctcgatgcgg cggccatcct gttgtgcgag tccgacggca
    26221 cgaccgacga agtccgggac gaagtcgcgc gcatggaagc cgtgttccgt cgggcggggg
    26281 ccacgcgcct gcaggtctcg cgcagcgagc aggagcgcct gctgttctgg gcgggccgca
    26341 agaatgcctt tcccgcggcg ggccgcatgt cgcccgacta ttactgcatg gacggcacca
    26401 ttccccgccg ccatcttgcg cgtgtgctgg gcgccatcga gcagatggaa gacgagtacg
    26461 ggctgcgctg tgccaacgtg ttccatgccg gtgacggcaa cctgcacccg ctcatcatgt
    26521 tcgattccaa caagcccgac gaagtcgatc gggccgagaa attcggcgcg gccattctcg
    26581 agctctgcgt gcaggtcggt ggcaccgtca ccggcgaaca tggcgtgggt atcgagaaga
    26641 tcaaccagat gtgcgtgcag ttcggccgtg aagagctcga cgcgttcttg gcgttgaaat
    26701 cggcgttcga tacggaaggg ttgctgaacc cggaaaaagt cattcccaca ctggcgcgct
    26761 gcgctgagta cggcaagatg catgtccatg gcggagacat ccgctttcct gaattgccgc
    26821 gtttctgagg gcgcaagatg gagtttgtgt taaccgagct gatcgaacgt gtcatgaccg
    26881 cgcgcgccgc acagaggccg gtccatatcc ggggtggcgg caccaaggat ttctatggcg
    26941 aaccgggcag tctccagggc gcgaccaacc actccgtgct ggatatgtcc gcctatcacg
    27001 gtgtcgtcaa ctatcagccc cccgaactcg ttctcaccgt gcgcgcgggc acgcggctgg
    27061 ccgaggtcga acgcctgctc gacgagcacg gccagatgct ggcgttcgag cctccccgat
    27121 tcggtccgga ctccaccgtg ggaggctgtg tggcggccgg gctgtcgggc ccgcgccgcg
    27181 ccgcggctgg tggtctgcgg gacttcatcc tgggggccaa gctgctcgac tcgtcgggct
    27241 cggtgctgga gttcggcggg gaggtcatga agaacgtggc gggctacgac atgtctcgtc
    27301 tgctcgcggg gtcgatgggc atcttcggcg cgttggtcga agtctcgatc aaggtcgttc
    27361 ccaagccctt ggtggagaaa acagcccggc tcgatgtcga tgaggcggga gcgctggcct
    27421 tgttcaacca gtggcgcggc aaacccctgc cggtgtcagc caccgcctgg gttcccggcg
    27481 aggcgcccgc cctctgggtg cgcctttccg gcagcgcgcc ggcggtcgat gcgggcgcgc
    27541 gtgtggtggg cggcacagag gcggactccg acgaggctgg tcccttctgg gacagcctgc
    27601 gggaccacac ccatccgttt ctgcggtcgt gcccgctttg gcgcctcgcg gtgcctcctc
    27661 aggtcgaccc gcttggactg ggaccttcgt tgattgaatg gaatggcggg cagcgctggt
    27721 tggctcaatc cctcgaggcg ggcgcactgc gtgagcgcgt ggcccggttg ggcgggcacg
    27781 ccacgctgtt tcggccgggc gccgataaca acgcgccggt gttccatccg cccgcgccag
    27841 gtttgatggc gatttcccgg cgcctcaagc acgcgctgga cccggtcggg atcttcaatc
    27901 ccaagcgcct gttttccgaa ttctgagata tcgccgtgca aacacatctt gccccctggg
    27961 cacaagacac caatttcggc caggacgccg acgccattct gcgtcgttgt gtgcactgcg
    28021 gtttttgcct ggccacctgc cccacgtacc aaattcttgg cgacgagcgc gacagcccac
    28081 gcggccgcat ctacctgatc aagcaagtgc tcgagggcgc cgaactcacg ggcgagacgc
    28141 aggagcacct cgaccgctgt ctcacgtgcc gtaattgtga aaccacgtgc ccatcggggg
    28201 tggagtatgg cgcgctggtc gatattggcc gcaatctggt ggatcgcaag gtcgaacgcc
    28261 cattccggca gcgcgtgtcg cgcgccttgc tgcgccagac gctgacgtcg cgatggttcg
    28321 gcacggcgtt ccggcttggg cggctgttgc ggccgctgct gccggaagtc ttgaaaggca
    28381 agattcccgc cgcccgccca gcgggtaaat tgccatcgaa gacctcatat gcgcgcacgg
    28441 tgctgatgcc agtggggtgc gttcagccgg ccatgatgcc gtccatcgac gcggccacct
    28501 tgcgcgtgct ggacgccctg ggcatcgggg tgcgcatgga gcccggcagt gggtgctgcg
    28561 gagccatcaa tttccacctc gacgcacagg atcaggcccg tggccagatg cgggccaaca
    28621 tcgacgcctg gctgccgctg ctcgagaacg gcaccgtcga ggccatcgtg atcaacgcat
    28681 ctggttgtgg cgcgatgatc aaggaatacg cgcatcatct caagcatgat ccggcctatg
    28741 ccgggaaggc acagcgcttg gtcgagaaag tggtcgatgt ggccgagatc gtcgcgcctc
    28801 acgctgtcga actggcacct cggttgcatg ggttgcccgc tcgcgtcgcg ttccatccgc
    28861 cctgcacctt gcagcattgg caaggcttgc gtggcgtttc cgaacggctg ctgaccgacc
    28921 tgggcttcaa tcttgtgtcg ttttcagaat cccacttgtg ctgtggctcg gcgggcacgt
    28981 attccgtgac ccaacccgag ctcgccaagt cgttgcgcgc acgcaaactc ggccatatcc
    29041 aggaggtgcg gccggactcc atcgtgtcgt ccaacatcgg ttgcatcgga catctgcaag
    29101 ggggcaccag tgtgcccgtg cggcattgga tcgagatcgt cgacgaggcc ttggccgcgt
    29161 cgaaagccaa ttagggtcta cgtagcatta gggtctgccc ggcgttactt taaaatacgg
    29221 catgagcact gtttccgtat caaaagggcg tgtcgtggtc ggcatgtctg gtggcgtcga
    29281 ttcctccgtc agcgcgtggc tgctcaagca gcagggctac gacgtggtcg gcctgttcat
    29341 gaagaactgg gaggacgacg acgattccga atattgttcc tcgcgacagg attggctgga
    29401 cgcggccagc gtggccgatg tcgtgggcgt ggagatcgag gcggtcaatt tcgccgccga
    29461 atacaaagac cgtgtcttcg ccgaattcct gcgtgagtat tccgcggggc gcacgcccaa
    29521 tcccgacgtc ctgtgcaacg ccgaaatcaa attcaaggcg ttcctcgatc acgccatgtc
    29581 gctgggggca gaccatatcg ccaccggcca ctatgcccgg gtgcgagcca tatcgaccgg
    29641 tcacagcacg gaatatcaac tgctgaaggc gctggatgcc tccaaggatc aaagttattt
    29701 tctgcacagg ttgaaccagt cacaactggc ggccacgttg ttcccgcttg gcgacatgcc
    29761 caagacagag gtgcggcgca tcgcgcgtga aatcggcctg ccgaacgccg cgaaaaaaga
    29821 ttccacgggc atctgtttca ttggcgagcg gccgttccgc gagtttctga gccgctacct
    29881 gccgacgagg cccggtccca tcaagacacc tgagggtgcg gtgatcggca tgcatcaagg
    29941 cttgtcgttc tatacgctgg gtcagcgcaa ggggctgggc ataggcggcg tcaaggggcg
    30001 ccaacgggac gatggcacgg ccgatgcgtg gtatgtggcg cgcaaggagc tggatacgaa
    30061 cacgttgtat gtggtgcagg ggcatgacca tccctggctg ctgagtgaca ccctgggcgc
    30121 gcaggatgcg agctgggtgg cgggctgtgc gccggcgcct ggcggctaca ccgccaaaac
    30181 ccgataccgc caggccgatg ccggctgctc actgaatgcc gatgcatcga acggtttctc
    30241 gctgcgtttt tcgcaggctc agtgggccgt gacggcgggg caatccgctg tgctgtacga
    30301 cggcgaggtg tgtctggggg gcggcatcat ttattgacac cggcgggtgg ggtgatgccg
    30361 ccgcgccagc aggaagaatc agggtttggg cggctcattg ctgatgaatg aggggcgggc
    30421 cgacagcttt tcgttgagac gctgcaggtt gctgtgttgc gaccgccagt cgagatcggg
    30481 caggcgcagg tcaaggtagc ccaatgcgca gccgacggcc acgtcggcca ggctgaagtt
    30541 gacgcccatg cagaacgggt gctcgtccag ccctttatcc atgaattcga gcgccgccgt
    30601 gattttcttg cgttggcgcg cgatccatgc ttcgctgaga tgctcggggg cgcgcttcgt
    30661 gcgttctatg tgaatggcgg ccgtggcgtc gagcaggccg tcggcgatgg cctcccagca
    30721 tttcaccgcg gcgcgttcgc gcccctgcgg aggaatgaga cgccccacgg gcgagagcgt
    30781 gtcgaggtat tccacgatga cgcgtgaatc gaacaagctg ccgttgtcgt ccatgatgag
    30841 gcacggaatc ttgcccaggg ggttgtgctg ggaaatccgc gtgttgtcgg cccacacatc
    30901 ctcgagaacg aattcgtaat caagtttttt ttcagccatg acgacgcgca ctttgcgtac
    30961 gtaggggctg gagagtgaac caatcagttt catggtgaaa tcgggagttt ctaacagaaa
    31021 aaagtataac aggcggtatc cggcggtccg gtatgccacc cggtgcggaa ccgcttgcct
    31081 gagacgttcg tgaaacgccg gcgagcctta actaacgagg ctagcatcat tttcccctta
    31141 ccggggatga ggcgcgcttt tttaccattg ttgtcagttt gatggattca gattgtcgta
    31201 tctctgagac agttctgacg tggatccgga acgtccgctc ggccggtttc cgattgtcgg
    31261 cttgcccgtt ccggaaggag ccccgcgtgg tcgtcgcctg tgttgccatc tgggggttgc
    31321 ccgggccgcg aatggtagaa ttcgagctgt tttcctcatg tgaatagcta tgcaaattgc
    31381 cgactcgctc agttccctga atgccttgtc ttccctcgat ggtcgctacg catcgaaggt
    31441 gggggcgctg cgcgaatccc tgtccgaggc cgggttcatg gcgcatcgcg tcgaagtcga
    31501 gatcgcctgg ctggaggggt tggcggaact ggggcttccc gagctttcgg ccttctcgtc
    31561 gcgggcgaag gcgttcctgg ccgatctcgt gcggtcgttc tccgaggccg atgccgcgcg
    31621 catcaaggaa atcgagcgaa ccaccaatca tgacgtcaag gcggtcgagt actggctgaa
    31681 agagcgcgtc gcgggcgatg cggaactgtc ggcggcggcg gagttcatcc acttcgcctg
    31741 cacgtccgaa gatatcaaca acacatcgca cgcgctgatg ctgacgcgcg cccgcgaaaa
    31801 cgtcctgttg ccccgccttg gcgagatctg cgcgctgctc aaggccttcg ctcgacaatt
    31861 cgccgaccag cccatgctgg cccgcaccca cggccagccg gccagtccga ccacgctggg
    31921 caaggaattc gccaacgtgg cggcccgcct cgaacgggcc atcgccgccg tctcggtcgt
    31981 gcagcccctg gcgaaaatga acggcgccac cggcaactac aacgcgcatc tgtccgccta
    32041 ccctgatgtc gactggccag gtttcagcgc cagggtgctg accggcctgg ggctgacgca
    32101 aaacccatat tccatacaga tcgagcccca cgactggatg gccgccctgt tcgatgccgt
    32161 ggcgcgcgcc aacaccatca tcctcgatct tgaccgcgac atctggggct acatttcgct
    32221 gggctacttc aagcagcagc tcaaggaagg cgaggtcggt tcatcgacca tgccgcacaa
    32281 ggtcaacccc atcgatttcg agaactcaga aggcaacctg gggctggcca acgccgtgct
    32341 gcgccacctg tccgacaagc tgccggtgtc gcgttggcag cgtgacctca ccgattccac
    32401 cgtgctgcgc aacctgggcg tcgcgttcgg ctattcagtg gtggcctggg acgcctgtct
    32461 gcgtggcctg aacaagctcg agctcaacgc ggcggccatc gacgccgaca tcgatgggtg
    32521 ctgggaggtg ctggccgaac ccgtgcagac cgtcatgcga cgctacgggc tgccccagcc
    32581 ctatgagcgg ctcaaggcgc tcacgcgcgg caagggcatc aatcgcgaag ggttggcgga
    32641 gttcatcgcc ggccttgatc tgccggaagc gcccaaggcg cggctcttgg cattgactcc
    32701 gcgcacctat gtcggtttgg cggctaagtt ggcgcgcgag gtttagagca aagcacaccc
    32761 cgattggcgc agattcgaat ttgcccctat aatctcgtct ttcccgattt cgggctgtta
    32821 gctcagttgg tagagcagcg gactcttaat ccgtaggtcg agcgttcgag ccgctcacag
    32881 cccaccagaa tgccaaaaca aaaggcccac cgtttacgcg gtgggccttt tgttttggct
    32941 caccaagttg cggtggtgcc atgggtgctc gttcacgaat ccgggaatct taaacgaacc
    33001 gcatccccag cagaaacaac cccgccgcga ggatcatggc cgctggcagc gtcagcaccc
    33061 acgccagcac gatgttgcgc acggtgcgcc cctgtattcc gctgccattg gccatcatgg
    33121 tgccggccac ccccgatgac agcacatgcg tggtggaaac cggcaggctg aagacgttgg
    33181 ccagcccgat cgagaacatg gcggtcagtt gcgccgacat ggcttgcgcg taggtgacag
    33241 tctgcttgcc gatcttttcg ctgacggtct ccacgattcg gcgccagccc accatggtgc
    33301 ccaggcccag cgccagcgcc acggcggcga tgacccacaa gggcgcgtat tccgtggtgg
    33361 tcgtcaggtc ggagcgcagg cgggcgaggt cggcgcgttc gggcgaggac aggtcctccc
    33421 tgtcttccac cttcttggcg atgttgtcca ggcacaatag gtagcggcgc accacgacgc
    33481 gttgcttacc ggtcagatca cggtagctgt tgacgccatt cagatcatgc agcagcagcg
    33541 acgtcgtgtg cgcggtgttg cgtgggtcgc aggcgggggt gtcggttttt gcccccgcct
    33601 gcggatcttg atgaagcggg gcgggtgtcc ggtgcgccat gatctgcgtc agatgggtgg
    33661 cggcggtgcg caccgagtcg atgtcgtagc tggtggcgtt cagattaagc acgaattgcg
    33721 ccggcacgat gccgatgagc accagcatga tcaggccgat ccctttctgg ccgtcgttgg
    33781 aaccgtgcac gtagcttagc cccagcgccg acagcaccag caccagacgg ttccagaacg
    33841 gggggtgttt tttgcctgct tcacgccgtt gatcgtgtgt ctggtgcatt ttcgagtccg
    33901 gcttccagcg ccgcgccgcc cattgcagca aggcggcaag ggcaaagccg ataagggggg
    33961 aaatcaccag ggacaggagg atttcgaccg ccttgcccca gttcacccct tcggcaagcg
    34021 ttgcgtgggt gagccaggcg ttggcgacgc cgacgcccag aatggagccg atcaaggtat
    34081 gcgaactgga ggcgggtatg cccaaatacc aggtacccag attccatgtg atggcggcgg
    34141 ccaacatgga gaacaccatg atcaggccat gcccggtgtc tatattgacc agcagttcca
    34201 ccggcagcaa gtgcacgatg gcataggcca cgcccacacc accggtcagc acaccgagga
    34261 aattgaaaat gcccgatagc atgacagcca ggtgcggtgt gatggccttg gttccgatcg
    34321 ccatggccac ggcgttggcg gtgtcgtgga agccgttgat gaattcgaag gtgagtacga
    34381 accccagcgc cagcacgagg ctggtgccta tccagaagtt caagccggag aatatgtcga
    34441 acatgggcgt ggtggtggaa aagtcgcgca attattgcag attttcccgt tggtccggcg
    34501 cctgccggcg gatattgccc ggtggcggac ggttatggcg cctgcttatg ccaaatgact
    34561 gtcggaaatg tggtttttat gctttctgcc tgatttgcaa caggtttatc aggttgttta
    34621 aagcagaaag cgggaaaata ctcccctttt tcctttttta gtttcacggg gcttgtgtga
    34681 ccacgacgaa tgacatcagc atggacccgc cggtattgcc gggggttcgc caagagggta
    34741 gcacagaagg gctgccgctc gcgccggatc ttagcctcag tctcgatgcg gcccacatgc
    34801 cgcggcgctc gacgctcata gacaagagca tgacgcgcat cattgtcatg tcgctgatcc
    34861 tcggggtggc ttccggattg atcgccaagg cgttgacggt gctgatcggc atcgtcacca
    34921 acctgtgctt ctacggccgc tggtcaaccg atttttccag tccggcggat gcggtgccgc
    34981 atctgggatg gtgggttgtc gtgatcccgg tgatcggcgg gttgatcgcg ggattcatgg
    35041 cgcgttgggg gtcgcgggcg atccgtgggc acggcattcc ggaagccatg gagcagatcc
    35101 tgctgcatga atcccggatt ccaccgcgca tcacctttct caaacccatt tcgtcagcca
    35161 tcgtcatcgg taccggtggg ccgttcggtg ccgagggccc catcatcgcc acggggggcg
    35221 ccctggggtc cctgatcggg cagatcatga aggtcaccgg cagcgaacgc aaggtgttgc
    35281 tggcggcggg cgcggcggcg ggtatggccg ccatcttctc cgcgccggtc gcggcggtgg
    35341 tgctggcggt ggaactgctg ttgttcgaac tgcgtgcccg ttcgctgatt ccggtcgcgc
    35401 tggcctgcgt ggccgccacg gcggtgcgct acgtgctgca ggggactgcg cccgttttcc
    35461 tcatgccgca tttcgactcc ccgggagcca tggcgctggt ggtctatgtc gtgatcggcg
    35521 cgttggtggg ggtgttcagc gtgtggacca cgcacatcgt ctatgccata gaagacggct
    35581 tcgaaaaact gccggtgcac tggatgtggt ggccggccct gggtggcctg gtggtgggcg
    35641 tcatcggcta tttcgctccg ctgaccctgg gggtgggcta ctccaacatc gaagacattg
    35701 taagcggccg tctgacggct ggcgcactgg tgttcctgtg cgtcatgaag ttcatttcct
    35761 ggtccatctc cctgggcagc ggcacatccg gcgggacgct ggctccgttg ttcaccatcg
    35821 gcggcgcgct gggcgggctg attggcatag ggttcgtgac gatcgcacca catcttgggg
    35881 tgaatcccaa tgttgccgcg ctggtcggta tggccgctat tttcgctggt gcgtcgaggg
    35941 cgttgttgac caccgtggtg ttcgcgctcg aaaccaccgg acagacagcg ggcctgttgc
    36001 ccttgttggg cgcctgcacg gcggcctatc tggtgtcggc attgatgatg cgtgacacca
    36061 tcatgaccga aaaaatcgcg cgacgcggcg tcaaggtgcc gtcggaatac ctggcggact
    36121 atctcgaccg catctcggtg ggggccgtgt gcgagcgtag cgtggtcact ctgaaagcaa
    36181 cccagacgct cgaagacgtg cgccgctgga tggcggatgg caagcctgaa tcgcagcacc
    36241 agggttaccc ggtcgtcggc gaaagcggga agctcttggg tgtgctgacg cgccgcacgt
    36301 tcttcaccga ggatgtcccc gcggccaccc cgttgggtga gttgattgtc cgaccgcccg
    36361 tagccgtgag cgaacgcaat accctacgcg aggcggcgga ccacatggca gaacagaacg
    36421 tcggccgcct gctcgtgctc gatggccaga acaacaagcg cgtagtgggc atgctcacac
    36481 gcagcgacct gatctcggcg cattcgcgtc gcctgaagga aggccaccgc cgccggcgct
    36541 acctgagtct gaaaacgtac cagcatatcc ggcgacaccg cgcgcggcgc gattgagccc
    36601 ggtcctcagc cgcgcccgtc ggtggcgttc ctggaacgcg tgatgcgcca ggcaaggatg
    36661 gcgcacgcta tctggaacac gcctccgcac atcatggcaa gccgcaggcc ggatgatgga
    36721 tcgtgtcgcg tcgcgtaaag cgtgccgagc accgccacac ccagggcggc gccggcgatc
    36781 cgcgccacgt tgatcagggc ggacgcggtg ccggaacgcg cggccggtgc ggaacc
//
LOCUS       DASTNK010000027        36748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_385065, whole genome shotgun sequence.
ACCESSION   DASTNK010000027 DASTNK010000000
VERSION     DASTNK010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 36748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36748
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385065"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..736
                     /locus_tag="VFR20_02050"
     CDS             104..736
                     /locus_tag="VFR20_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEU0229330.1"
                     /translation="MAKKKFSKEWIQQHVNDPYVKLAQQKGYRARAAFKLLEILDAEG
                     LLRRGNIVVDLGATPGSWSQVARERLLGKAGVIDGRVIALDLLPMEPIAGVEFILGDF
                     REQAVLDELETRLNGGKVDLVISDMAPNLSGVASADSARIQHVCELALDFAGRHLVPD
                     GTLIVKAFHGSGFSQIVESFKQSFVRVVERKPKASRSKSAETFLVGSRLK"
     gene            871..2775
                     /gene="ftsH"
                     /locus_tag="VFR20_02055"
     CDS             871..2775
                     /gene="ftsH"
                     /locus_tag="VFR20_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU0229331.1"
                     /translation="MNNSFSKVAIWMVIALVLFTVFKQFDGHSAVTQSVTYTQFMNDA
                     QAGRINKVDIQGDTIYVTPDAGRPYSLTSPGDLWMVPQLVKYGVQVSGKAREEPSFLT
                     SLFISWFPMLLLIGVWIFFMRQMQGGGKGGAFSFGKSRARMLDENTNQITFADVAGCD
                     EAKEDVQELVDFLREPAKFQRLGGRIPRGVLMVGSPGTGKTLLAKAIAGEAKVPFFSI
                     SGSDFVEMFVGVGAARVRDMFENAKKQAPCIIFIDEIDAVGRQRGAGLGGGNDEREQT
                     LNQLLVEMDGFETGQGVLVIAATNRPDVLDPALLRPGRFDRQVVVSLPDIRGREQILK
                     VHMRKVPLATSVDALVLARGTPGFSGADLANLVNEAALFAARRNGRTVEMHDFEKAKD
                     KIIMGAERRSMIMPEEERKNTAYHESGHALVARMLPKTDPVHKVTIIPRGRALGVTMQ
                     LPESDRYSMDKERLLNTIAVLFGGRIAEEVFMHQMTTGASNDFERATMIARDIVTRYG
                     MTDSLGPVVYAENEGEVFLGRSVTKTTHVSEATMQKVDSEIRAIIDEQYGVARKLIEG
                     NRDKMEAMAKALLEWETIDAEQIEDIMAGKPPRPPKTMAPSSGPQDSSLPPPPSGLPA
                     ADGSAATTPV"
     gene            2792..3631
                     /gene="folP"
                     /locus_tag="VFR20_02060"
     CDS             2792..3631
                     /gene="folP"
                     /locus_tag="VFR20_02060"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU0229332.1"
                     /translation="MSPSFRCGRFELRLERPLVMGIVNVTPDSFSDGAEFLDPDVAVA
                     HARQLVAEGADILDIGGESTRPGAQPVDANAELARVLPVVEALRDVGVPLSVDTSKAD
                     VMRRVLVAGADMINDVYALRQPGALEAVAGSDCGVCIMHMQGEPRSMQVAPQYDDLLA
                     DVQAFLQTRVEVVFAAGIDPSRVVTDPGFGFGKTKKQDFSLLQGLAQTRVQALPMLLG
                     LSRKSMIGHVTGRPVRERLAGSIAGMLAGVARGAQIVRVHDVAATVDALKVWRAVEFG
                     VTE"
     gene            3628..4977
                     /gene="glmM"
                     /locus_tag="VFR20_02065"
     CDS             3628..4977
                     /gene="glmM"
                     /locus_tag="VFR20_02065"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU0229333.1"
                     /translation="MSDGRKYFGTDGVRGEVGGATINAEFALRLGYAAGGVLARQMPG
                     RGRPAVLIGKDTRISGYMLESALEAGLSAAGVDVLLAGPVPTPAVAYLTRALRLVAGI
                     VISASHNPYQDNGIKFFSAQGMKLPDELESEIEQAVDRPVGCVGSEALGRARRIDDAA
                     GRYIEFCKSTFPTEFDLSGLKIVVDAANGAAYHIAPHVFRELGAEVHEVGCKPDGFNI
                     NEGVGALYPDQLIERVRAEGAQVGIALDGDADRVQMVDGAGRLYNGDELLYAIVRDRM
                     RHSRVEGVVGTLMTNFGFEKSMQALGVGFDRAKVGDRYVLERMLALGWQYGGESSGHL
                     LCLDRHTTGDGIIAALQVLASMRRSGETLDRLVADLKMYPQKMVNVPLEPGVRWQDHA
                     GLMAAKLKVEHDLNGRGRVLIRASGTEPKLRLMVEAEDAILAEQGVRELLAVNLARA"
     gene            5027..5809
                     /locus_tag="VFR20_02070"
     CDS             5027..5809
                     /locus_tag="VFR20_02070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HEU0229334.1"
                     /translation="MLELARIPPNSPSTGEPFSGSRTPGLVMGLARTNDELESIQRLR
                     YKVFTEEMGAVFPDAQDSIDADRFDPWCEHIMVKEGQSGAVVGTYRFMSPVNARKAGG
                     FYSESEFDLSGLGERRLNMAEVGRSCTHADHRNGAVIMLLWSGVSRLMIRNGYRYLLG
                     CASVSLRDDGATAAEVWRRAGELGRIVDEPKVVPLHRYPIERLDNALPARVPPLIKGY
                     LKLGATVCGEPAWDPDFNTADFPILLDMPAMDNRYRRHFGFI"
     gene            complement(5836..7326)
                     /gene="ppx"
                     /locus_tag="VFR20_02075"
     CDS             complement(5836..7326)
                     /gene="ppx"
                     /locus_tag="VFR20_02075"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEU0229335.1"
                     /translation="MDHLLAAVDLGSNSFRLSIGKVVQHDNIAQIYAIDNMKESVRLA
                     AGLDESKRLDEPTIERALAVLRRFRERLDGFHPNRVRAVATNTFRVARNAPEILPRAE
                     AALGFPIEVIAGQEEARLIFSGVANELRPSDQRRLVIDIGGGSTEIIIGKGFEPLHMA
                     SLYMGCVSYTQRFFAGGVITEERLLDAELTARQKLEGIARQYRKTGWQEAYGSSGTAK
                     GLIAVLTDGGMSKSGITLEGLMLLRERLIRDGRVRLENLPGLREDRAPVLAGGLAIMI
                     AAFKELSIDRMAAGDGALRVGVLYDLLGRDSAHDKRDETVRQFIRRYHVDTRQALRVR
                     TLALEFFRQTELPDCPEKQELVRTLGWAADMHEIGLSIAHADYHKHGAYILEHADMPG
                     FSNDDQVLLATLVLGHQGKLNKLRAREPTRAGWMALLSLRMAVLLSRRREGPSAVPVR
                     LELDHQTIRAHVRTSWLATHALTEYALQRELREWNRTDFLFEVVPA"
     gene            complement(7484..8176)
                     /locus_tag="VFR20_02080"
     CDS             complement(7484..8176)
                     /locus_tag="VFR20_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEU0229336.1"
                     /translation="MKTIGILGGMSAASTQTYYRELCDMTRKRLGGLHSPELLIRSLD
                     FDTIEALQKSGQWDRAGAILNTEAKALENGGAHVLVLATNTMHKLADQMMAGVSIPFL
                     HIAQATAGRIVNAGLSKPGLMATAFTMEQAFYTDGLRQAGLSPMVPGDADRAIVHGII
                     YEELCKDIRRPESREAFGRIAQRLIDKGADCLILGCTEIGMLLNQGNVSAPIFDTTLI
                     HCEAALDFALEA"
     gene            8281..9093
                     /locus_tag="VFR20_02085"
     CDS             8281..9093
                     /locus_tag="VFR20_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEU0229337.1"
                     /translation="MVDASVNATAGFGIKTCFIVFLLVGYGWNYRWRVVTLRLSCACH
                     PRMAKPQFGDPLLPAWLVRPRWLPTLVFDDMTDNLDRYNKLSIGVHWLTVILLIAVYA
                     LMELRGVFPKDSAPRDLMKTWHYMLGLTVFVIFFVRLASRLVFRSPPITPEPSALMNR
                     LAKYMHVALYIFLFAMPLLGWFTLSAKAEAIPFFGLHLPALMGPDKTLGRDIKDVHET
                     LGTIGYYLIGLHVAAALFHHYFMRDNTMLRMWPGFGRRFGGGARSLETDRRG"
     gene            9165..9962
                     /locus_tag="VFR20_02090"
     CDS             9165..9962
                     /locus_tag="VFR20_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010096707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HEU0229338.1"
                     /translation="MDTPLSPVDLDSLRNARELLENPGLAARMTNLIGAPVELGLKWL
                     PEGWRSKISDITQAALRKATDAAIVTLDDRPGEGSSNHWHKLGAAVSGGVGGFFGLAG
                     LAVELPISTTLMMRSIADIARSEGESLGHPDARRACLEVFALGGKTKTDDATDSGYYA
                     IRVALAQSVAQATEYLGTHVVVEKGTGPALVRLITVVADRFGVQVTEKAAAQAIPALG
                     AAGGAVINTLFIGHFQSMARGHFTVRRLERKYGAEAVRAAYMALGEV"
     gene            10182..12134
                     /locus_tag="VFR20_02095"
     CDS             10182..12134
                     /locus_tag="VFR20_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HEU0229339.1"
                     /translation="MTDRNEGTERHRSEFVESLVDYSRKQQAQKWEGGFNDYLRDIVA
                     KNPARAARSSHQYIWDMMCWRGLEAPKQNGASTHYKLFEDDLFGIDQALERVANYFKA
                     ASEGSEVGRRLLLLLGPPSGGKSSLVTLLKRGLEAYSHTDDGALYAIQESPIQESPLL
                     LIPQGKRAEFRDTYGVQISGEVSPFTRTLLDEQYGGDFTRVPVHRIFIDEAGRDAVGT
                     YAPHDPTTADIADLVGSVDLSKVAEYGDEGDPRAWSWSGAVYAGNRGLLEMIEILKVK
                     REFLYLLLTLTQEQVVKVSRFPLIYIDETIVAHTNMAEFRRFLQEKENEALLDRMVIV
                     QVPYTLCYTDEARIYRKLTSATPSFRLVHLDPHALQVAAVFAILTRLKPSERQDLDLS
                     KKLRIHAHESVEGISDAEAERIVREQPDEGMDGVSPRFIVNALAGAISQSESKSLTSM
                     EVMLALKDAIESDARMDSKVKKQWIDFLVTTRKEFYNRWVKEDVHKALFVSFQDEAQE
                     LLDKYLDEVEAVLENRKVDDPITGEERDADERFLRSVETKIGISDSGKTSFRQEIVRK
                     ALNAYKRGVKFTLAEHARLREAIEQYLFEERRDVLRLVTSTTRADTEAMEKISAVEER
                     LVNDYGYDAHSAHEALNYVTTLLAQE"
     gene            12157..13353
                     /locus_tag="VFR20_02100"
     CDS             12157..13353
                     /locus_tag="VFR20_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HEU0229340.1"
                     /translation="MTKPAYRQLSNLAHAHRWYGLFSRGARDWLRHNEKVRDAVREQL
                     PEMIAGADVLGRPSNHTVLIPVHFLEHYRFRLSTSNEQSGVGQGGGKPKDVYQQGQGE
                     GSDAGQAGAGGSGNGELRFLLELRIEDIVDWIWEELHLPDLKPRTSDALRDEDLVREG
                     WSRHGPNSRLDRRRTMKEAIKRRHAQPAGAAPFSDEDLRFRQLARRQRPATDAVVALL
                     LDVSGSMDAERRKLAKSFFFWAVQGLRRQYGSALELIFIAHTNEAWEFTEEQFFQATA
                     TGGTTASSAFQLALDIFDKRYPTRRYNHYLFYASDGENFADDRALADGLLCKLGDIVN
                     FMGFVETPQNPHETGLSETGRLFRSLESRNYPVGTYTVHERGDVWNAVRRFFQRQASV
                     GVGEEA"
     gene            13355..14746
                     /locus_tag="VFR20_02105"
     CDS             13355..14746
                     /locus_tag="VFR20_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEU0229341.1"
                     /translation="MPTTHAQDLEQLAHYMPKIEALARRDGLDYDLVDFELVPNTFMM
                     EVAVYGLPIRMPHWSFGVRYIHQHVQHRMGGSHIFEVVFPGNPNRAYLVTDNGLAQNT
                     LVTAHVLGHADFSKNNALFARMQKQVGYHIVTETAERARSIQDAIEEYGQRRVEMVLD
                     AALALEQHIDVDQPIHRPDYPTHILPKPGVKPTGFHARFAGLPGEKAPPPQQRRRAPI
                     PPSPESDLLWFIARYAPDMEGWERDIFLAVREESFYFYPVFACQIMNEGWASYWHARL
                     LREADFLPASLYVDAIKSHSDVIRPYASGKNIALSLNPYNVGFIMWEHIIENEGLDRA
                     LRIRAEEDDFSFIRNHLDETLAEKLQLLSYRTHQDSATGLNIEIQNTDIDALREQLIA
                     PKFNFAAPHVQAVELSSDGSLMLRQDHVADGRGLDIERAQKVLEYIKLVWRRPVALET
                     VDGNGNEKTLSIQ"
     gene            14794..15753
                     /locus_tag="VFR20_02110"
     CDS             14794..15753
                     /locus_tag="VFR20_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="reductase"
                     /protein_id="HEU0229342.1"
                     /translation="MNATGGRRGLGFAPAHDQPIAYMQRTREWYLALGYGNPYVWAHY
                     AEVPFHVLGKSLSSATVTLITTAAPFQGGKGPQGPGAPYNAATKFYEVYSLPTERLPD
                     LRISHVAIDRKHTSMEDSGTWFPLAALRTLAQEGRVGRLAPRFHGAPTNRSQRHTLDV
                     DCKEILRRCREDHVDAAILVPNCPVCHQTLSLIARHLEAHGIATVIMGCAKDVVEYCG
                     VPRFLFSDFPLGNAAGKPHDPRSQRRTLELALQLLDSAPAPRTTVQNPQRWSDDPDWK
                     LDYCNVEKIDQGELTTLQEESERARRLARDVREASLADGRSTE"
     gene            complement(15882..16109)
                     /locus_tag="VFR20_02115"
     CDS             complement(15882..16109)
                     /locus_tag="VFR20_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229343.1"
                     /translation="MRQILIIVGLVLLVAGIWVVAGHGSYQNTETVIKIGSAALKTTH
                     DEAIPKWLGIAGIVVGAILALGGFAGWFKRK"
     gene            16767..17363
                     /locus_tag="VFR20_02120"
     CDS             16767..17363
                     /locus_tag="VFR20_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229344.1"
                     /translation="MTNDNRLALVGVIKATCQYVAENDLAKARDTIVSRYPFAPKANV
                     GRSYGPEFMTKVFLRDGFIDRYKGTNLIFPPVLRLLSHYLPDDFPYHRNWKVDATHPA
                     YWELLPTIDHVVPVARGGQDAEPNCVSCSMLTNSIKSAWTLDELGWQLQPAGDADRWD
                     GMMGWFIKQVEQHPELLRVGEVGPYLKRWYGPAKASRG"
     gene            complement(17658..17733)
                     /locus_tag="VFR20_02125"
     tRNA            complement(17658..17733)
                     /locus_tag="VFR20_02125"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17698..17700),aa:Ala,seq:cgc)
     gene            17940..20081
                     /locus_tag="VFR20_02130"
     CDS             17940..20081
                     /locus_tag="VFR20_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU0229345.1"
                     /translation="MRKHESADSAQKAHPIRPAQRAARAGWLARRTMAFRLVLATLAV
                     TVLVMGVMTAIMAWRAQQEAVATVNREMGSALTGVDQSLQLVYASATAQVGHLIPSFE
                     HAMGGVPVLGATTSETGSAGRVPNLVVNGILINNDISALLGVKRSTGADAEVMVRSGG
                     HWVRVSTMQEDAQGKQMLGTSVAPTDLVARTLDAGTDYSGLIKRDGKWYVMGIKSLHG
                     KDGKVYGGLSIWASVNDDVSRLLQWVDTVRVAKYGTLAILHHTDAGWQYVAGAGAQAG
                     DALSAHFSAGEVARLNTLFQAKTGFADVPIGPDGQSTFVAWQGVKNWNWLMVGFGAKS
                     AFMAASYRSLALQIGMILVGTILIAALIGWLSAATLHPVREMIAHMARFGRGDLTADL
                     PEVPARSRNEVHALFGSLRQMQASLGETVSSVRRGVEEIDVGSREIAAGNTDLSSRTE
                     QQAASLQETAASMEQLAATVRQNADNAHQANGLASSASSVAVRGGEIVSRVVDTMAGI
                     SQGSKKIAEIVSVIESIAFQTNILALNAAVEAARAGEQGKGFAVVASEVRSLASRSAQ
                     AAKEVKMLIDESSSKVDEGARYVEQAGSTMTEIVASVQRVTDIMGEIAAASDEQSSGI
                     GQVNQAVSQMDEATQQNAALVEQAAAAAASLQEQVQTLTQAVAVFRLRGGAQAQPVGQ
                     AGDSGRMPGGREEVEPTTHGGRLLLPATGAA"
     gene            complement(20121..21170)
                     /gene="selD"
                     /locus_tag="VFR20_02135"
     CDS             complement(20121..21170)
                     /gene="selD"
                     /locus_tag="VFR20_02135"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEU0229346.1"
                     /translation="MTTAHPPRLTELSHGGGCGCKISPGVLAELLKNLPRTQAFPGLM
                     VGPETSDDAAVYRLNDEQAIIATTDFFMPIVDDPYDFGRIAAANALSDIYAMGGTPIL
                     ALALVGMPINVLPHETIARILEGGASLCAEAGIPIAGGHSIDSVEPIYGLAALGVAHP
                     DHIRRNQDAQAGDALILSKPLGVGILSAALKKNLLDEDGYAQMIASTTQLNRSGPALA
                     RLPGVHAMTDVTGFGLLGHTLELCRGAGLRAHLRHDDLPWLSPVRAFAEAGVFTGASG
                     RNWASYGEQVDLDARLPAVARTLLTDPQTSGGLLVSCAAGVADQVLAQLRADGFGQAT
                     VIGAMVEGGIGVSVA"
     gene            complement(21170..22231)
                     /gene="mnmH"
                     /locus_tag="VFR20_02140"
     CDS             complement(21170..22231)
                     /gene="mnmH"
                     /locus_tag="VFR20_02140"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEU0229347.1"
                     /translation="MIPYLANLEQLAEFDEIVDVRTPAEFEDDHIPGALNAPVLDNAQ
                     RAEIGTLYKTAPFDATRRGAAMVAHNIARHLDTLFADRPRQWRPLVYCWRGGKRSGSM
                     TAWFNLIGWRARQLEGGYKTYRRHVLQRLDDLPGQFHYVVLTGSTGSGKTRLLHALAQ
                     AGAQTLDLEGLSRHRGSLLGAWPGQDQPSQKTFESALAQVLSTFDPGRPVFVEAESRR
                     IGQITLPTAMLDSYRRGACVDVQTPLAERIGFLLEDYAHLFSDKATFKQQLGRLAVLH
                     GNDTIARWHGLIDADAAPELFRDLITRHYDPAYARSTSRSFGDMSGALAFEFHPCAED
                     TRGQADALLARVSGLTEHI"
     gene            22419..23765
                     /gene="argG"
                     /locus_tag="VFR20_02145"
     CDS             22419..23765
                     /gene="argG"
                     /locus_tag="VFR20_02145"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU0229348.1"
                     /translation="MTTILEHLPVGQKVGIAFSGGLDTSAALLWMRNKGAVPCAYTAN
                     LGQPDESDYDSIPKRAMQYGAEKARLIDCRAQLVAEGIAALQCGAFHVSTAGVTYFNT
                     TPIGRTVTGTMLVAAMKEDNVNIWGDGSTYKGNDIERFYRYGLLTNPGLQIYKPWLDQ
                     QFIDELGGRAEMSEYMRQAGFDYKMSAEKAYSTDSNMLGATHEAKDLEHLNSGIRIVQ
                     PIMGVAFWRDDVPVAREEVTIRFEQGLPVALNGVEYRDSVELMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGLALLFIAYERLVTGIHNEDTIEQYRDNGRKLGRLLYQG
                     RWFDSQAIMLRETAQRWVARAITGEVTLELRRGNDYSLLDTRSPNLTYKPERLTMEKG
                     ESTFSPRDRIGQLTMRNLDITDTRDKLFVYVNAGLLAPAGKAALPHLKDDSEEQGK"
     gene            complement(23966..24919)
                     /gene="argF"
                     /locus_tag="VFR20_02150"
     CDS             complement(23966..24919)
                     /gene="argF"
                     /locus_tag="VFR20_02150"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU0229349.1"
                     /translation="MSNAALQTGPLRHFLQFSDFTKAEILYVLERARLIKNKFKHYEP
                     HHTLHDRTLAMVFEKASTRTRVSFEAGMYQLGGSVIHLTTGDSQLGRAEPIEDTARVI
                     SRMVDVVMIRTFEQTRLERFAAHSRVPVINGLTNEFHPCQILADIFTFIEHAGSIEGK
                     IVAWVGDANNMAYTWLQAAEMLGFRLHVSAPEGYRLDPQRVGEPAADALQEFDDPMQA
                     CRGAHLVTTDVWTSMGYEAENDARRKAFADWCVDADMMAVAAPDAVFMHCLPAHRGEE
                     VTGEVIDGPQSVVWDEAENRLHVQKALMEFLLLGQLDTPSA"
     gene            25106..25447
                     /locus_tag="VFR20_02155"
     CDS             25106..25447
                     /locus_tag="VFR20_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HEU0229350.1"
                     /translation="MTHIVQQTIIHGTTLDGQRFRPSDWAERLAGVMSQFRPAGCPGG
                     RLSYSPYVVPTLLDGVRCVVVDGRLRDLEPLAWKFVMDFASDNHLRTSEREFDAASDD
                     WGGTRPSPLAD"
     gene            complement(25742..26005)
                     /gene="rpsT"
                     /locus_tag="VFR20_02160"
     CDS             complement(25742..26005)
                     /gene="rpsT"
                     /locus_tag="VFR20_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU0229351.1"
                     /translation="MANSAQARKRARQSVERNKHNASLRSMLRTSIKRVRQAIEAGDK
                     TVAADVFKKATGTIDRIADKKIIHKNKAARHKSRLAAAIKALG"
     gene            complement(26293..27855)
                     /gene="murJ"
                     /locus_tag="VFR20_02165"
     CDS             complement(26293..27855)
                     /gene="murJ"
                     /locus_tag="VFR20_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEU0229352.1"
                     /translation="MSLLRSTATVSGFTLVSRITGLLRDILIASTFGAGPLTDAFWVA
                     FRIPNLLRRLFAEGAFSQAFVPILGQARLENSHDGVRILLDRVALLLTAILMLVTVIG
                     VIAAPWVVTLMASGLRGATRHAEFHAAVWMTRVMFPYIVFMSLIAFASGVLNTWSRFA
                     VPAFTPVLLNLSMIGACLFLTRHFDVPIYALAAGVMIGGAAQLVVQWIAISRLGLAPR
                     YSPRVRQAWRDPMVKRIMHQMMPAILGVSVAQISLLINTNIATWLTPGSVTWLSFADR
                     LMEFPTALLGVALGTVLLPVLSAAHARQDHTGYSELLDWGLRLMLLLGLPAALGLALL
                     SKGLVATLFNYGAFNAHDVLETRLAVMAYSVGIVGLLSIKILAPGFYARQDIRTPVKI
                     AVVVLILTQLFNVIFVPRFAHAGLALSVGLGASVNALCLLVMLRRRGIYRPGPGWRRF
                     RLRIIPALAALSALLIVADQRIDWLQLGATHHGGQRGLWLLAVLAASVIVYFTVLFVC
                     GFRPRDFTRRGR"
     gene            complement(27874..28632)
                     /locus_tag="VFR20_02170"
     CDS             complement(27874..28632)
                     /locus_tag="VFR20_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEU0229353.1"
                     /translation="MEIRNKVFIVTGGASGLGAGTVRMLVAEGAKVVLADIQDEAGQK
                     LAAELGQAYVHCDVTQEADGQAVVAAALAAGPLFGLVNCAGVAPASRTVGKNGPHSLD
                     LFRKVVGINLIGTFNMCRLAADAMSRNEPESTGERGVLINTASVAAFDGQIGQAAYAS
                     SKAGVAGMTLPLARDLSKLGIRCMTIAPGIFGTPMIFGMPQEVQDSLAASIPFPPRLG
                     TPEDYASLVNQIIRNSMLNGETIRLDGAIRMAPK"
     gene            complement(28698..29360)
                     /gene="adk"
                     /locus_tag="VFR20_02175"
     CDS             complement(28698..29360)
                     /gene="adk"
                     /locus_tag="VFR20_02175"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU0229354.1"
                     /translation="MRLILLGPPGAGKGTQATFITQHFGIPQISTGDMLRAAVKAQTP
                     LGLEAKKIMDTGGLVSDDIIIGLVRDRLRQPDCEPGYLFDGFPRTIPQADALKDARIA
                     LDFVLEIDVPEDNIIERMSGRRTHPASGRSYHVKFNPPKTPGIDDVTGEPLVQRDDDK
                     EETVRHRLAVYRKQTRPLVDYYSGWAATGAADAPAYRKLSGLGSVDEIKERIFQALSV
                     AA"
     gene            complement(29466..30230)
                     /gene="kdsB"
                     /locus_tag="VFR20_02180"
     CDS             complement(29466..30230)
                     /gene="kdsB"
                     /locus_tag="VFR20_02180"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEU0229355.1"
                     /translation="MSFIAIIPARAASTRLPGKPLLDIAGKPMVVRTAEQASRSLASR
                     VVVATDSSDIQAAVRHHGFEALLTRTQHPTGTDRLAEAAEALALTDDTIVVNVQGDEP
                     LIDPAQINAVANLLGDNPEAAIATCAAPITDAQALFNPNAVKVVRDHRGRALYFSRAP
                     IPWARDALADGERHLAAGLPALHHIGLYAYRAGFLKRFPSLPVGSLETFESLEQLRAL
                     ENGYVIMVGLTDSPPAAGVDTEADLTRVRQVFANAP"
     gene            complement(30227..30442)
                     /locus_tag="VFR20_02185"
     CDS             complement(30227..30442)
                     /locus_tag="VFR20_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEU0229356.1"
                     /translation="MESRLLEILVCPLCKGPLHFNREQQELICNTDKLAFPIRDGIPV
                     MLEQEARDLHSAPSGPATGAATDDQRA"
     gene            complement(30478..31569)
                     /gene="lpxK"
                     /locus_tag="VFR20_02190"
     CDS             complement(30478..31569)
                     /gene="lpxK"
                     /locus_tag="VFR20_02190"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEU0229357.1"
                     /translation="MKPSARLATLAHRVWRGKGLASTVLLPFSWITRLVVAHKRQRFA
                     RPDARQRGRSPVVVVGNIYVGGTGKTPVVIALAQGLRERGWTPGVISRGYGVASSQAP
                     RVGTGHLDPARFGDEPALIALRTGVPVGVHPRRILALRALEAAHPEIDVVIADDGLQH
                     IELGRDIELAVQDARGIGNGRLLPAGPLREPADRLGQIDYLVVNLEPGQSDPASTGGV
                     PTVGMRLFPSHVEHLATRRSVDWSTWLIEHGRTPANAVAGIGRPERFFGMLEACGLAL
                     AHTVALPDHAAFQTSPFDALGPGPILITAKDAIKCGAFTNDDRLWVVHATPRFTDSHW
                     LDDIARRLRDIVENKDSRSETPGASAHYT"
     gene            complement(31566..31976)
                     /locus_tag="VFR20_02195"
     CDS             complement(31566..31976)
                     /locus_tag="VFR20_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEU0229358.1"
                     /translation="MNFRRSRTDDEPEINLVPLIDVLLVILIFLAATTSFTRFSQIKV
                     HLPQASADAPQPQAMNLAISQTGVYALDGRYVQGTSVDDLAQALKAEAAQRRQGTLII
                     DADAQSTHEAVVRAMLAARQAGIERVDFATQSTP"
     gene            complement(31973..32578)
                     /locus_tag="VFR20_02200"
     CDS             complement(31973..32578)
                     /locus_tag="VFR20_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEU0229359.1"
                     /translation="MLEILRVAGWPIWLLVATSVLGLAVIIERFLSLRSALVLPARLP
                     AQVADLVKKGTDTPETLDRLASSSPLGQVLAEVLIHRQESGALRVEAVEDAGKDVAHR
                     LNRYLPALATIAVVAPLMGLFGTVVGMIEIFAAYTPAGGDPSQLARGISIALYNTGFG
                     ILIAIPALIFHRYFRSRVDYFLHQMEREASALNRYISRTPQ"
     gene            32816..33886
                     /locus_tag="VFR20_02205"
     CDS             32816..33886
                     /locus_tag="VFR20_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEU0229360.1"
                     /translation="MFLKNAWYVAALGSEVGRSLLAVRLLGEDIVLYRKLDGKVAALE
                     DACPHRKLPLSMGRLKEDTVECGYHGLVFDCSGQCVHAPGLARAPRAAVARAYPVEER
                     YGMAWVWMGDPARANPDSIVEVDHWGDPAWGVNRGDAMTVQCHYLYITDNLLDPSHVS
                     WVHQTSFGNADIIGEPLETDVREEGVRVSRWMRDVEVAPFYAKFVRFKGHCDRKQHYE
                     VRFPSQAVIRAIFTPAGTATDSDPFHPDVFLMDSYNFLSPVDESTTRYFWFQMRNFSP
                     DDEGVSRQFSDDVRHAFEEDRAVLEAVHRGMARSPSRMNLPLDSGPLRFRRKLGEMID
                     AELAEAGLEADVDMSGPRLYTT"
     gene            34322..35674
                     /gene="xseA"
                     /locus_tag="VFR20_02210"
     CDS             34322..35674
                     /gene="xseA"
                     /locus_tag="VFR20_02210"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU0229361.1"
                     /translation="MIDNSEAVLTVSELNRRVGRLLEGEFPKVWVKGEISNFTRAASG
                     HWYFTVKDEGAAVRAVMFRGRAASVGFVPKAGERFEFRGSVTLYEPRGDYQLQVDMLR
                     RAGLGNLHEAFVLLKNRLADEGLFDGERKRPIPAMPSAIGVVTSLAAAALHDVLSVLA
                     RRVPHVPVILYPAPVQGADAAARLVQAVDAAVRRDEVDVLLLVRGGGSLEDLWSFNDE
                     GLARALAACPIPIISGVGHETDFTIADFVADLRAPTPTAAAELSCQSRDDCLALVRRA
                     LDALTRRQRRHLDTAALRLDRAASALVSPAQRVARQRESLESLGARLARAAALAMERC
                     GVRLDHARARMRRSGPDTRRARQDLARCLERLGAAGGNLVPRRLDALSGSVQTLQALD
                     PRRVLGRGYALVRNRDGELVKNALDLNIDERLNLEFGQGRAEVSVVSAQGSTSADKCP
                     "
     gene            35803..36381
                     /gene="sodB"
                     /locus_tag="VFR20_02215"
     CDS             35803..36381
                     /gene="sodB"
                     /locus_tag="VFR20_02215"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HEU0229362.1"
                     /translation="MAFTLPPLPYDMDALAPHISKETLEFHYGKHHQSYVTNLNNMVP
                     GTEFESASLEQIIKKASGGLFNNAAQVWNHTFYWNSLSPKGGGEPKGALLAAINAKWS
                     SVAAFKEAFSKSAAGNFGSGWTWLVKKPDGSLDIVNTSNAATPITGADVPLLTCDVWE
                     HAYYIDYRNARPKYLEHFWNLVNWDFAAKNLG"
     gene            36600..>36748
                     /locus_tag="VFR20_02220"
     CDS             36600..>36748
                     /locus_tag="VFR20_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741777.1"
                     /note="catalyzes the thiolytic cleavage of
                     beta-ketoadipyl-CoA to succinate and acetyl-CoA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEU0229363.1"
                     /translation="MTTQAYICDAIRTPFGRYGGSLAQVRADDLAAAPIKALMARNPG
                     VDWSRL"
BASE COUNT         6741 a        10902 c        12087 g         7018 t
ORIGIN      
        1 tcaccgatca ggacgactgg ccgcaagggg tgcgcggcgg cacgtagggt gctgcgctct
       61 ttggcggtga tatcgagttt tggcatattg agattgtacg ggaatggcca agaaaaaatt
      121 ctcaaaagag tggatacagc aacacgtcaa tgacccgtat gtcaaattgg cgcaacaaaa
      181 agggtaccgg gcccgggcgg cgttcaagct gctcgagatt ctcgatgccg aagggttgtt
      241 gcgccgcgga aacattgtgg tcgatctggg cgcgacgccg gggagctggt cccaggttgc
      301 gcgcgagcgg ctgctgggca aggctggtgt catcgacggg cgcgtcatcg cgctggatct
      361 gcttcccatg gagcctatcg ccggcgtgga gttcatcctg ggtgacttcc gcgagcaggc
      421 cgtgcttgac gaactggaaa cccgcttgaa tggcggcaag gtggaccttg tcatttccga
      481 catggccccc aacttgtccg gtgtggcctc ggccgattcc gcccgcatcc agcacgtgtg
      541 cgaactggcg cttgattttg ccggtcgcca tctggttccg gatggaacgt tgatcgtcaa
      601 ggctttccat ggcagcggct tttcccagat cgtggagtcg ttcaagcagt ctttcgtgcg
      661 ggtggtcgag cgcaagccaa aggcgtcacg cagtaaatcc gcagaaacct ttttggtcgg
      721 aagccgtctc aaatagtatg gaaaagagtc acaaaaggct ttcaaacaag attcgtcctc
      781 tcgggttaga atgagtcatt gacaagtctg gtttgatgcc gtatttgcgg ggcggcggcc
      841 ggacgtgatg tgctatgtag gaggttggct ttgaacaact cgttttccaa ggtggcgatc
      901 tggatggtga tcgcgcttgt gctgttcacc gtattcaagc agttcgacgg ccatagcgcg
      961 gtgacccaat cggtcaccta tacgcagttc atgaacgacg cgcaggccgg ccgcatcaac
     1021 aaggtcgata tccagggtga caccatttac gtcacccccg atgccggccg gccatacagc
     1081 ctcacgtcgc ctggcgacct ctggatggtg ccccagctgg tcaagtatgg tgtgcaggtt
     1141 tccggtaagg cacgggaaga gccttctttc ctgaccagcc tgttcatctc ctggttcccg
     1201 atgctgctgc tcatcggcgt gtggatattc ttcatgcgac agatgcaggg gggtggcaag
     1261 ggcggtgcgt tcagtttcgg caaatcgcgc gcccgcatgc tcgatgaaaa caccaaccag
     1321 atcaccttcg cagacgtcgc cggctgcgac gaagccaagg aagacgtcca ggagttggtc
     1381 gactttctgc gtgagcccgc caagttccag cgcctggggg ggcgaatccc ccgcggggtg
     1441 ctcatggttg gctcgcccgg caccggtaaa accctgctcg ccaaggccat cgccggcgaa
     1501 gccaaggtgc cgttcttcag catttccggt tccgacttcg ttgaaatgtt cgtcggtgtg
     1561 ggtgcggcgc gcgtgcgcga catgttcgaa aacgccaaga agcaggcgcc gtgcatcatc
     1621 ttcatcgatg aaatcgacgc ggttggacgc cagcgtggcg ccggactggg cggcggcaac
     1681 gatgagcgcg agcaaaccct gaaccagctg ctggttgaaa tggatggctt cgagaccggt
     1741 cagggcgtgc tcgtcatcgc cgccaccaac cgccccgacg tgcttgatcc ggcgttgctg
     1801 cgtccgggtc gtttcgaccg ccaggtggtg gtctctctgc ccgacatccg tggccgcgaa
     1861 caaatcctca aagtacacat gcgcaaggtg ccgctggcca ccagcgtgga cgcgcttgtc
     1921 ctggcgcgcg gcacacccgg cttctcgggt gccgacttgg ccaacctggt caacgaggcc
     1981 gccctgttcg ccgctcggcg caacggccgc acggtcgaaa tgcacgactt cgaaaaggcc
     2041 aaggacaaga tcatcatggg cgccgagcgc cgctccatga tcatgcccga ggaagagcgc
     2101 aagaacaccg cttaccacga atccggccat gccctggtcg cgcgcatgtt gcccaagacc
     2161 gacccggtcc acaaggtcac catcatcccg cgcggccgcg cgctgggcgt gaccatgcaa
     2221 ctgccggaaa gcgaccgcta cagcatggac aaggaacgtc tgctcaacac gatcgccgtg
     2281 ctgttcggtg ggcgtatcgc tgaagaagtc ttcatgcacc agatgaccac aggcgcgtct
     2341 aacgacttcg aacgtgccac catgatcgcc cgcgacatcg tcacgcgcta cggcatgacc
     2401 gattcgctgg ggccggtcgt ctacgccgaa aacgaaggcg aggtcttcct tggccgcagc
     2461 gtaaccaaga ccacccacgt ctccgaggcc accatgcaga aggtcgactc ggaaatccgg
     2521 gccatcatcg acgaacagta cggcgtggcg cgcaagctca tcgaaggcaa ccgcgacaag
     2581 atggaggcca tggccaaggc gctgctcgaa tgggaaacca tcgatgcgga gcaaatcgaa
     2641 gacatcatgg ccgggaagcc gcctcggccc ccgaagacca tggcgccttc gtccggccca
     2701 caagactcct ccctgccgcc gccacccagt gggctgccgg cggcggatgg cagcgcggcc
     2761 acgacgcccg tctaggtctc ctttcgcgac aatgagtcca tcttttcgat gtgggcgctt
     2821 cgagcttcgg ctcgagcgcc ccttggtaat ggggatcgtc aacgttacac cggattcgtt
     2881 ctccgatggc gccgaattcc tggatccgga tgtggccgtg gcgcatgcgc ggcaactggt
     2941 tgccgaaggt gccgatatcc tcgacatcgg gggcgaatcc acgcgtcctg gcgcacaacc
     3001 tgtagatgca aatgcagaac tcgcccgagt tctgccggtt gtcgaggcgc tgcgcgatgt
     3061 gggcgtgccc ctgtcggtcg atacctccaa ggcggatgtc atgcggcgcg tgctcgttgc
     3121 gggtgccgac atgatcaacg acgtctatgc gctgcgccag cctggcgctc tggaagccgt
     3181 ggctggctct gattgcggcg tgtgtatcat gcacatgcaa ggggagccgc gcagcatgca
     3241 ggtggcaccg cagtacgatg acctgctggc tgacgtgcag gcgtttcttc agacgcgcgt
     3301 cgaagtggtg tttgccgctg ggatcgatcc ctcacgggtc gtgaccgacc ccggattcgg
     3361 attcggaaaa accaaaaagc aggatttttc attgttgcaa ggcctggcgc aaacacgtgt
     3421 acaggcgctg cccatgttgc tcggtctatc gcgcaaatcc atgataggcc atgtcaccgg
     3481 gcggcctgtg cgtgagcggt tggctggcag tatcgccgga atgctggcag gtgtggcgcg
     3541 cggtgcgcag atcgtgcgtg tgcacgacgt cgccgccacg gtggatgcgc tcaaggtatg
     3601 gcgcgcggtg gagtttggag tgactgaatg agtgatggcc ggaagtactt tggcaccgat
     3661 ggggtgcgtg gtgaagtggg gggcgcgacg atcaacgccg aattcgcgct gcgtctgggg
     3721 tatgcggccg ggggggtgct ggcccggcag atgccggggc gtgggcgacc ggccgtgctc
     3781 attggcaagg acacccgcat ttccggctat atgctcgaat cggcgctcga ggctggcctg
     3841 tccgccgccg gtgtcgacgt tctgctggcc ggccccgtgc ccacgcccgc tgtggcgtac
     3901 ctgactcgcg ccctgcgcct ggtggcgggt attgtcatca gcgcctcgca caatccgtac
     3961 caggacaacg gcatcaagtt tttctcggcg caaggcatga agctgcccga cgaactcgaa
     4021 tccgagatcg agcaggccgt cgatcggccc gtggggtgcg tcgggtccga ggcgcttggg
     4081 cgcgcgcgcc gtatcgatga tgccgcgggg cgctacatcg agttctgcaa gagcacattc
     4141 cccaccgaat tcgacctgtc gggcctgaag atcgtggtgg acgccgccaa cggcgccgcc
     4201 taccacatcg ctccccatgt gttccgggag ctcggcgccg aagtccacga ggtcggctgc
     4261 aagcccgacg gcttcaacat caacgaaggg gtgggcgcgt tgtatcccga ccaactcatc
     4321 gagcgggttc gggccgaggg cgcccaggtc ggcatcgcgc tggatggcga tgccgatcgt
     4381 gtgcagatgg tcgatggcgc gggccgtttg tacaacggcg acgaattgct ctatgccatc
     4441 gttcgcgacc gcatgcggca ttcgcgggtg gagggcgtgg tcggcacgct gatgacgaac
     4501 ttcggattcg agaaaagcat gcaggcgctg ggcgtcggct tcgaccgcgc caaagtgggc
     4561 gatcgctacg tgctggagcg tatgctggcg ctgggctggc agtatggcgg cgaaagctcc
     4621 gggcatttgc tttgccttga tcgccatacc accggtgatg gcatcatcgc ggctttgcag
     4681 gttctcgcca gcatgcgccg tagtggcgaa acgctggatc gcctggtcgc cgatctgaag
     4741 atgtatccac agaaaatggt caacgttccg ctggaacccg gtgtgcgctg gcaggaccat
     4801 gccggactca tggcggccaa gctcaaggtg gaacacgatt tgaatggtcg cggccgcgtc
     4861 ctcatccgcg catcgggcac cgagcccaag ctgcggctca tggtcgaggc cgaagacgcc
     4921 atccttgccg aacaaggagt gcgcgaactg ctggccgtga atctggcccg cgcctgacca
     4981 aaccgcacca atgccccgac atcgtcagcg tatctgaact tctcctatgt tggaattagc
     5041 gcgcattccc ccaaattcac ccagcaccgg cgagccgttt tcaggctcgc gcacaccggg
     5101 cctcgtcatg ggcctggcgc gcaccaatga cgagctcgaa tccatccagc gtctgcgcta
     5161 caaggtgttc accgaggaga tgggggcggt gtttcccgac gcgcaggaca gcatcgacgc
     5221 cgatcggttc gatccgtggt gtgaacacat catggtgaag gaaggccagt caggcgcggt
     5281 ggttggcacg taccgcttca tgagcccagt caatgccagg aaggcgggcg gtttctattc
     5341 agagtccgaa ttcgacctgt cagggttggg tgagcgacgc ctgaacatgg ccgaagtggg
     5401 gcgctcttgc acgcatgccg accatcgcaa tggcgcggtg atcatgctgc tgtggtcggg
     5461 tgtgtcgcgt ctcatgatac gcaacggcta ccgttatttg cttggctgcg ccagcgtcag
     5521 tttgcgcgac gatggtgcca cggccgccga agtctggcgc agggcgggcg aactgggccg
     5581 catcgtggac gagcccaaag tggttccgtt gcatcgttac ccgatcgagc gcctggacaa
     5641 cgccttgccc gcgcgggtgc cgccactgat caaggggtat ctcaagctgg gcgccacggt
     5701 atgtggcgag ccggcttggg atcccgattt caacacggcg gattttccga tactgctgga
     5761 catgcccgcc atggacaatc gctaccggcg ccattttggt tttatctgac ggtttcgatg
     5821 atgtgtaaag tgtgatcagg ccggcaccac ttcgaacagg aaatccgtcc ggttccattc
     5881 gcgcagttcg cgttgcagcg catattccgt gagcgcatgg gtggccagcc acgatgtgcg
     5941 cacatgcgcg cggatggtct ggtggtcgag ttccagccgg acgggaacgg cgctggggcc
     6001 ttcgcgccga cgcgacaaca gcaccgccat gcgcaggctg agcagcgcca tccatcccgc
     6061 ccgcgtcggt tcacgtgccc gcagcttgtt gagcttgcct tgatgcccca gcaccagcgt
     6121 ggcgagcagg acctggtcgt cgttggaaaa gcccggcatg tcggcgtgtt ccaggatgta
     6181 tgcgccgtgc ttgtggtagt cggcatgggc gatcgacagc ccgatttcgt gcatatcggc
     6241 cgcccacccc agggtgcgca ccagttcctg tttttcgggg cagtccggaa gctcggtttg
     6301 ccggaaaaac tcgagcgcca gcgtcctgac gcgtagtgcc tggcgggtgt cgacgtggta
     6361 gcgccgaatg aactgtcgca cggtttcgtc gcgcttgtcg tgcgcggagt cgcggcccaa
     6421 taaatcatac agaacgccca cccgcaaggc cccgtcaccc gcagccatgc gatcgatgct
     6481 caactccttg aaggcggcga tcatgatggc cagaccgccc gccaataccg gagcgcggtc
     6541 ttccctcagg cctggcaggt tttcgaggcg gacgcggccg tcgcggatca atcgttcgcg
     6601 caacagcatc aacccctcta gagtgatgcc ggacttcgac atgccaccgt cggtcagcac
     6661 cgcgatcaag ccttttgccg ttcccgacga accgtaggct tcctgccagc cagtcttgcg
     6721 gtactgcctg gcgatccctt caagcttctg ccgcgcggtc agttccgcat ccagcaggcg
     6781 ctcctcggtg atgacgccgc ccgcgaagaa gcgttgtgtg tagctgacgc accccatgta
     6841 caacgacgcc atgtgcagcg gttcgaaacc cttgccgatg atgatctcgg tggacccgcc
     6901 gccaatgtcg atgacgagcc gccgctgatc ggacgggcgc agttcgttcg ccaccccgga
     6961 aaatatcagg cgcgcctctt cctgtcctgc gatgacttct atgggaaagc ccagtgcggc
     7021 ctcggcgcgc ggcagaattt ctggcgcgtt gcgcgcgacg cgaaatgtat tggtggccac
     7081 cgctcggact cggttggggt ggaaaccgtc gagccgctcg cggaaccgtc gcagcacagc
     7141 caaggccctt tctattgtcg gctcgtcaag gcgtttcgac tcatccagtc ccgcagccaa
     7201 gcgcacagat tccttcatat tgtcgatagc gtagatctgc gcgatgttgt cgtgctgcac
     7261 gactttcccg atggagagcc tgaaactgtt ggagccaagg tctacggcgg caagcaaatg
     7321 atccatgggg ggtgggagtg gtgggagaga gtgtggtcga attataaaga gtgtgtcatg
     7381 cttttcgcgt tcagggcaca cgttggagct ttggctgcgg aacgagactg tattggtcga
     7441 gaaggccgga tgatgtgtta ttcacggcct ttgtcgagga cggctatgcc tccagggcga
     7501 aatccagcgc cgcttcgcaa tgaatgaggg ttgtgtcgaa tatcggggcg gacacgttgc
     7561 cctgattgag tagcatgccg atttcggtac agcccagaat caggcagtcg gctcctttgt
     7621 cgatcaggcg ttgtgcgatt cttccgaaag cctcccggct ttcgggcctt ctgatgtcct
     7681 tgcacagttc ttcgtaaata atgccgtgca cgatcgcccg gtcggcatcg cccggcacca
     7741 tagggctcag tccggcctgc ctgagcccat cggtgtagaa cgcctgctcc atggtgaagg
     7801 ctgttgccat cagccccggt ttgctcagcc ccgcattgac gatgcgcccc gcagtggcct
     7861 gggcgatatg caggaatggg atgctcacgc ccgccatcat ttggtcggcc agcttgtgca
     7921 tggtgttggt ggccaggacc aggacgtgcg caccgccatt ctcgagggct tttgcttcgg
     7981 tgttgagaat cgcccctgcg cggtcccatt ggccgctttt ctgaagcgcc tcgatggtgt
     8041 cgaagtctag cgagcggatt agcagttcgg gagagtgcag cccgcccagc cgcttgcgtg
     8101 tcatgtcgca aagttcccga taatacgtct gggtcgacgc cgccgacatg ccgccaagta
     8161 tgccgattgt cttcatgttg ttgcctacgc tccctggccg cgcatcgggc cggataaaaa
     8221 attcatcggt tcctggatcg tgtctatgcc ggtcttggcg cagaccgcgt ccttgcgacg
     8281 ttggttgatg ccagcgtgaa cgcaacggcc gggttcggga taaaaacctg tttcattgtc
     8341 ttccttctcg tgggttatgg ctggaattat aggtggcgtg ttgttacgct aaggctgtcc
     8401 tgtgcttgcc accccagaat ggccaagcca caatttggtg accctcttct tccggcttgg
     8461 ttggtgagac ctcgctggtt gccgaccctg gtgttcgatg acatgactga caacctcgat
     8521 cgttacaata aactgtcaat tggtgtgcac tggctgacag tcatactgtt gatcgccgtt
     8581 tacgcgctga tggaattgcg gggtgttttt cccaaagaca gcgcgccgcg cgacctgatg
     8641 aaaacttggc attacatgct tggcctgaca gtgttcgtca tattcttcgt gcggctggcg
     8701 tcgcgcctgg tgtttcgctc tccgcccatc acgcccgagc cttcggcgct gatgaaccgg
     8761 ttggcgaaat acatgcatgt cgcgctgtac attttcttgt tcgccatgcc gctgctgggt
     8821 tggttcacgc tcagcgcgaa ggccgaggcc ataccctttt tcgggttgca cctgccggcg
     8881 ctgatggggc cggacaaaac cctggggcgc gacatcaagg atgttcacga aaccctcggc
     8941 accatcggct attacctgat cggcctgcat gtggcggccg cgctgtttca tcattacttc
     9001 atgcgcgaca atacgatgct gcggatgtgg ccgggatttg gacggcggtt tggtggcggc
     9061 gccagaagcc ttgagaccga ccggcgtggg taggctccgg ccagtgtgtt ggcggggcgg
     9121 ggccatatcg tgcggcggca tgtgaacata tcggcggaca atccatggac acgcctttga
     9181 gcccggttga cctcgattcc ttgcgcaacg ctcgcgaact gctggagaat cccgggcttg
     9241 ccgcgcgcat gacgaacctg atcggtgcgc cggttgaact ggggttgaag tggcttcccg
     9301 agggctggcg gtcgaaaatc agcgacatca cccaagccgc gctgcgcaag gccaccgatg
     9361 cggccattgt cactttggac gacaggcctg gagaaggcag ctccaaccac tggcataagc
     9421 tgggggccgc ggtgagtggc ggggtgggcg ggtttttcgg tctggctggc ttggcggtgg
     9481 agcttcccat atcgaccacg ctgatgatgc gatccatcgc tgacatcgcg cgcagcgaag
     9541 gcgaatccct ggggcacccg gatgcccgcc gcgcctgtct ggaggtcttc gcactgggcg
     9601 gaaagacgaa aacggacgac gccacggata gtggctatta cgctatccgc gtagcgttgg
     9661 cccagtcggt cgcccaggct acggaatatc ttggtacgca tgtggttgtg gaaaaaggca
     9721 ctggcccggc cctggttcgc ctgatcaccg tggtcgctga ccgattcggc gtccaggtca
     9781 ccgagaaggc ggcggcccag gccatccccg cgttgggggc cgcagggggc gcggtgatca
     9841 acacattgtt catcggccat ttccagagca tggcacgggg ccatttcacg gtgcgccggt
     9901 tggagcgcaa atatggggca gaggctgtgc gcgcggcgta tatggcgctg ggtgaagtct
     9961 gacggaaggg gctgcctgac ctgatatcaa gtcagcgtcc acaacaaaca atacatatcc
    10021 cttcctcttg gcgcgaagaa gtcgtatgct gaacttcaac cgcccatgcg ggtccaaccc
    10081 attagagatg gatttttcaa ggttgagcca ccactgatac cgacccattc gcacaacgcc
    10141 agtgtcagcg ggtagcccaa catcatgctt ggagaagagc cgtgactgat cgcaatgaag
    10201 gtacagaacg gcatcgttct gagtttgtcg agtcgctggt ggactattcg cgcaagcagc
    10261 aggcgcagaa gtgggaaggc ggcttcaacg attacctgcg tgacattgtc gccaagaatc
    10321 ccgcgcgggc agccagatcc agtcatcagt acatctggga catgatgtgc tggcgcggtc
    10381 tcgaggcccc gaagcaaaac ggcgccagca cccactacaa attgtttgag gatgatttgt
    10441 tcggcatcga tcaggcgctt gagcgggtcg ccaattactt caaggcggcc agcgagggtt
    10501 ccgaggtcgg gcggcggctg ttgcttttgc tgggaccccc gtcgggcggc aagtccagcc
    10561 tggtgacgtt gctcaagcgc ggcctcgagg catacagcca caccgatgac ggggccctgt
    10621 acgcgatcca ggagagcccc atccaggaat cgccgctgct cttgatccca cagggcaaac
    10681 gggccgagtt ccgggacacc tacggggtcc agatcagcgg cgaagtgtct cctttcacgc
    10741 gcacgctcct tgatgagcag tatggcggtg atttcacccg cgtgccggtg catcgcatct
    10801 tcatcgatga ggcgggtcgc gacgcggtgg gcacttatgc gccccacgat cccaccaccg
    10861 ccgacatcgc tgacctggtg gggtcggttg atttatcgaa ggtggctgaa tacggggatg
    10921 agggcgatcc gcgcgcctgg tcgtggtcgg gcgccgtgta tgccggcaac cgcggcctcc
    10981 tggagatgat cgaaatcctg aaggtcaagc gcgagttcct gtacctgctc ttgacgctga
    11041 cccaggagca ggtcgtcaaa gtatcgcggt tccccctcat ctacatcgat gagaccattg
    11101 tcgcgcacac caacatggcg gagttccgcc ggttccttca ggaaaaggaa aacgaggcat
    11161 tgcttgaccg catggtcatc gtgcaggttc cctataccct gtgctacacc gatgaggcgc
    11221 gcatctatcg caagctgacc tccgccaccc cttcgttccg gctggtgcat ctggatccgc
    11281 acgcgctgca ggtggcggcc gtgttcgcca ttctcacgcg gctgaaaccc tccgagcggc
    11341 aggatctcga tctcagcaag aagttgcgca tccacgcgca tgaatcggtc gagggcattt
    11401 ccgacgccga ggccgaacgc atcgtccggg aacagcccga cgagggcatg gatggtgttt
    11461 caccacgctt catcgtcaat gcgctggccg gcgccatcag ccagtccgag agcaagagcc
    11521 tgacctccat ggaagtcatg ctggcgctca aggatgcgat tgaatccgat gcacgcatgg
    11581 attccaaagt gaagaagcaa tggatcgatt tcctggtcac cacgcgcaag gagttctata
    11641 accgctgggt caaggaagac gtgcacaagg cgctgttcgt gtccttccag gacgaggcgc
    11701 aggagcttct ggacaagtac ctggatgagg tcgaggcggt gctggagaac cgcaaggtgg
    11761 acgaccccat caccggtgaa gaacgcgacg ccgatgaacg gttcctgcgc agcgttgaaa
    11821 ccaagatcgg catatccgat tccggcaaga cctcgttccg ccaggagatc gttcgcaagg
    11881 ccctgaatgc ctacaagcgc ggtgtcaagt tcacgctcgc ggagcatgcg cgcctgcgcg
    11941 aagcgatcga acaatacctg ttcgaagagc ggcgcgacgt gctgcgcctg gtcacctcga
    12001 ccacccgcgc cgacaccgag gccatggaaa aaatctcggc ggtcgaggag cgcttggtca
    12061 acgactacgg ctacgatgcg cacagcgcgc acgaagcgct caactacgtg accacgctgc
    12121 tggcgcagga atagtccaga cacatgagtc gccatcatga ccaagcctgc ataccggcag
    12181 ctttccaatc tggcgcacgc gcaccgttgg tacggcctgt tttcgcgcgg cgcccgtgac
    12241 tggctgaggc ataacgaaaa agtgcgcgac gccgtgcgcg agcagctgcc cgagatgatc
    12301 gcgggggcgg acgtgctggg ccgcccctcc aaccacaccg tgctgatccc ggtgcatttc
    12361 cttgagcact atcgcttccg cctgagcacc tccaatgaac aatcgggtgt ggggcagggc
    12421 ggtggcaagc cgaaagacgt gtatcaacaa gggcaaggag agggcagcga tgccgggcag
    12481 gctggcgcgg gtggcagcgg caatggcgag ttgcgtttcc tgctggagct caggatcgag
    12541 gacatcgttg actggatatg ggaggagctg cacctgcccg atctcaagcc gcgcaccagc
    12601 gatgcgctcc gggacgagga cttggtgcgc gaggggtgga gccggcacgg ccccaattcc
    12661 cgtctggatc gacgccggac catgaaagag gcgatcaagc gtcggcatgc ccagccggcc
    12721 ggcgcggcgc cgttctccga cgaagacttg cgtttccggc aactggcgcg gcggcaacgc
    12781 ccggcgaccg acgcggttgt ggcattgctg ttggatgtgt ccggatccat ggatgcggaa
    12841 cggcgcaaac tggccaaatc atttttcttc tgggcggtgc aggggctgcg ccggcaatat
    12901 ggcagcgcgc tggagctcat cttcatcgcg cacaccaatg aagcctggga gttcaccgag
    12961 gagcaattct tccaggcgac cgccaccggc ggcaccaccg cctcaagcgc ctttcaattg
    13021 gcgctggata tcttcgacaa gcgctacccc actcggcgct acaaccatta cctgttctat
    13081 gcctccgacg gcgagaactt cgccgatgac cgggccctgg ccgatggcct gctgtgcaag
    13141 ctgggcgata tagtcaactt catggggttc gtggaaaccc cccagaatcc gcatgaaacc
    13201 gggctttccg aaacaggccg cctgtttcgc tcgctggaat cgcgtaatta cccggtgggc
    13261 acctataccg tgcacgagcg cggtgatgtc tggaatgccg tgcgtcgctt cttccagcgc
    13321 caggcaagtg tgggcgtggg cgaggaggcc tgagatgccc accacgcatg cgcaggatct
    13381 ggagcaactt gcccattaca tgccgaaaat cgaagcattg gcgcggcgcg acgggctgga
    13441 ttacgatctg gtcgattttg aactggtgcc caacaccttc atgatggaag tggccgtgta
    13501 cggcctgcct attcgcatgc cgcactggtc ttttggcgtg cgctacatcc atcagcacgt
    13561 gcaacaccgg atgggcggtt cgcacatctt cgaggtggtg tttcccggca accccaaccg
    13621 cgcctacctg gtcaccgaca acgggctggc gcaaaacacg ctggtcaccg cgcatgtgct
    13681 gggacacgcc gatttctcca agaacaatgc cttgttcgcg cgcatgcaga agcaagtggg
    13741 ttatcacatc gtgaccgaga cggcggagcg ggcgcgcagc attcaggacg caatcgaaga
    13801 gtacggtcaa cggcgtgtgg aaatggtgct ggacgcggcg ctcgcgctgg aacagcacat
    13861 cgatgtcgac cagccgattc accggcccga ttacccgacc cacatcctgc ccaagcccgg
    13921 cgtgaagccc actggtttcc acgcccgctt cgccgggctg cccggcgaaa aggccccgcc
    13981 accgcagcag cggcggcgcg cacccattcc acccagtccg gagtcggatt tgctgtggtt
    14041 catcgcgcgg tatgcgcccg acatggaggg ctgggaacgg gatattttcc tggcggtgcg
    14101 ggaggaatcg ttttacttct accccgtgtt cgcttgccag atcatgaacg aaggctgggc
    14161 cagctactgg catgcgcgcc tgctgcgtga agccgacttc ctgcccgcgt cgctatatgt
    14221 agacgccatc aagtcgcatt ccgacgtcat ccgcccctac gccagcggaa agaacatcgc
    14281 gctttccctc aacccctata acgtcggctt catcatgtgg gagcatatca tcgagaacga
    14341 ggggctggac cgcgctttgc gcatccgtgc cgaggaagac gacttcagct tcatccgcaa
    14401 tcacctggac gagaccctgg ccgagaaact gcaattgctg agctatcgaa cacaccagga
    14461 ctccgcgacc gggctcaaca tcgagattca aaacacggac atcgacgcct tgcgcgagca
    14521 actgatcgcg cccaagttca atttcgccgc gccgcatgtg caggcggtgg agctgagcag
    14581 tgacggcagc ctgatgctgc ggcaggatca tgtggccgat ggccggggcc tggatattga
    14641 gcgcgcgcag aaggtgctgg aatacatcaa gctcgtgtgg cgtcggccgg tggcgctgga
    14701 aacggtcgat ggcaacggca atgagaagac gctgtccatc cagtgacgga tatttggatt
    14761 attctgaagg ccgctcaatc gaaggaagca gccgtgaatg caaccggtgg ccggcgcggc
    14821 cttgggttcg ccccagccca tgaccagcct attgcgtata tgcagcggac ccgcgagtgg
    14881 tacctggccc tggggtatgg caacccgtac gtctgggctc actacgcgga agtaccgttc
    14941 catgtgctcg gcaaatcact gtccagcgcc accgtcacgc tcatcaccac agcggcgccg
    15001 ttccagggag gcaagggccc gcaaggcccg ggggcgcctt acaacgcggc gacgaaattc
    15061 tacgaggtgt attcactccc caccgaacgt ctccccgatc tgcgcatctc tcatgtcgcg
    15121 atcgatcgca agcacaccag catggaggat tccggaacgt ggtttccgct ggccgcgttg
    15181 cgcacattgg cgcaagaggg acgagtcggt cggttggcgc cgcggtttca cggcgccccg
    15241 acgaaccgca gccagaggca tacgctggat gtggattgca aagagatcct gcggcgctgc
    15301 cgggaggatc acgtcgacgc cgccatcctg gtgcccaact gtcccgtctg tcaccagacg
    15361 ctcagcctga tcgcccggca tttggaggcc catggcattg ccactgtcat catggggtgc
    15421 gccaaggatg tcgtcgagta ttgcggcgtt cccaggtttt tgttcagcga tttcccgctg
    15481 ggcaacgccg ctgggaagcc gcacgacccc aggtcccaga gacgcacgct ggagcttgcc
    15541 ctgcagttgc tggatagtgc gcccgcaccg cgcacgacgg tgcagaatcc gcagcgctgg
    15601 agtgacgatc cggactggaa attggattat tgcaacgtgg aaaaaatcga ccagggcgaa
    15661 ttgacaacct tgcaggagga atccgaacgg gcaagaaggc tcgcccgcga cgtgcgtgag
    15721 gcgagcctgg cggacgggcg ttcgacagaa tgatcaccgc ccgcggggcg gcggcccgtc
    15781 gcggccgcgc gctccgcctc ctgtgaggcg cagttccgat atgccgccgc ggatgtcgtg
    15841 agtgccgaag cggctgtatg cgttgaggag tttgggccgc gttatttgcg tttgaaccag
    15901 cccgcgaaac cgcccagggc caaaatagca cccaccacga tgccggcgat gcccagccat
    15961 ttcgggatgg cttcatcgtg cgtcgttttc agggcggccg aaccgatttt gatgacggtt
    16021 tcagtgtttt gatagctgcc atggcccgct accacccaaa tgcccgccac gagcagaacg
    16081 aggccgacaa tgatcaagat ctggcgcatg tctggttttc catcgtgttt gaatatccaa
    16141 aaatttacca cgcttgcgaa aaccgaatgg gtttgtcgtc tgaggccagg gcccatgacg
    16201 ccccacgcgg ggatcgccca aaacaaaacg cccgacagaa atctgtcagg cgtgcgttct
    16261 ggtggcggga ggccatgaag gttcgaacgg ttctttttga cacggttccg aatcagcgcg
    16321 tgtgggagga acggcatcca tgtgaaacta ataccggact gtgttgcggc tcgttctgtg
    16381 gatttgtgag tgcggccaca caagcgccct aacaactctt cggaggcggc tttgattggc
    16441 ggcgttcact cgcgcagagt acgattacga tagcggtttt gttacaaaaa ctgcccaata
    16501 cgctacaagg agaggggtaa tgagttggaa tgcatgtcag cttccaccaa ttgatttccg
    16561 atgggatcac cttcgtgcgt tcagcgacgt tgttcaagaa tgcaagttgc gggacacgcg
    16621 ggctctaatg gattcggatg cccaccaatc tcgagtcacg gttgatgtcc tccaaacggc
    16681 gttcatgcga gctgtttcaa tagatcctaa atactactga aagcggtgtg tgggcatgca
    16741 cgattcaatt tttttgtcgt ccattcatga cgaacgataa tcgcctggct ctggtgggtg
    16801 tgattaaagc gacgtgtcag tacgttgcgg aaaatgatct ggctaaagcg cgagacacta
    16861 ttgtctctcg gtacccgttt gcacccaaag cgaacgtggg caggagctat ggccccgagt
    16921 tcatgacgaa agtatttctc cgggacggtt ttattgacag gtataaagga acgaatctga
    16981 tctttccacc cgtgttgcgt ttgctgtcgc attatctccc ggacgatttt ccgtatcaca
    17041 ggaactggaa ggttgacgcc acgcatcctg catactggga actgttaccc accatcgacc
    17101 atgtcgtgcc tgtggcacga ggtggtcagg atgcagagcc gaactgtgtg tcctgctcaa
    17161 tgctgacgaa cagcatcaag tcggcctgga cgctggatga gctgggctgg cagttgcaac
    17221 cggcgggcga tgccgatcga tgggatggca tgatgggctg gtttatcaaa caagtggagc
    17281 agcacccgga gttgctgcgg gttggcgagg tggggccgta tttgaagcga tggtatggcc
    17341 cagccaaggc gagtagaggt tagctgtttt ctacaccact ccctgcgtag cttggtgact
    17401 ggagtatcga aacgtcacgg agatcgctgt gacaactggg cgctgtctgt gccgtgggga
    17461 gaatttccag atctgatctc cactggcaac acgttcgcgg tatttttttg ggaagctgag
    17521 acgaaaagag ggccgaactg ctggaaacgg tcgagttggc caggcgcccg tggtacgcac
    17581 agagccagga gagtggcgca gcacgcgggg agtccaccaa aaaaaacgcc tgactgagaa
    17641 cagtcaggcg tgcattctgg tggagacgag gaggatcgaa ctcccgacct tcgcattgcg
    17701 aacgcgacgc tctcccagct gagctacgtc cccgaagccg tggattttag cagagtcttt
    17761 cgaaccgtgc tttcacgaaa gtctcctgcg agtagcctca tggaaattgc ccgcctcccg
    17821 gccaagccct aaaccttctc cggttcctgc cgtttacacg tgacagaaca aaaccgcgtt
    17881 gcggggccgt ctgcgtaccg acgggcggcc tgagcggcag ataaaggatg aaggcaggca
    17941 tgcgcaaaca tgaatcggcg gattccgctc aaaaggcaca tccgatcaga cccgcccagc
    18001 gggcggccag ggcgggatgg ttggcgcgtc gcaccatggc ctttcgtctg gtgctggcaa
    18061 cgctggcggt cacggtgctg gtgatggggg tcatgaccgc catcatggcc tggcgggctc
    18121 agcaagaggc cgtggccacg gtgaaccgcg agatgggttc cgcgctcact ggcgtggacc
    18181 agtcgctgca gctggtgtat gcctcggcga cggcgcaggt gggccatctg atcccctctt
    18241 tcgagcatgc gatggggggt gtgccggtgc tgggtgccac aacgtctgaa accggctcgg
    18301 ccgggcgggt gccgaacctg gtcgtcaacg gcattctcat caacaacgac atcagcgcct
    18361 tgctgggggt caagcgcagc accggggcgg atgccgaggt catggtgcgt tcgggcggcc
    18421 attgggtgcg cgtgtccacc atgcaggaag atgcacaggg caagcagatg ctgggcacgt
    18481 cggtggcgcc gacggatctg gtcgcgcgta cgctggacgc cggcaccgac tattccggcc
    18541 tgatcaaacg cgatggcaag tggtatgtca tgggcatcaa gtcgttgcac ggcaaagacg
    18601 gcaaggtcta tggcggcctg agcatctggg ccagcgtgaa cgatgacgtc agccgcttgc
    18661 tccagtgggt ggacaccgtg cgcgtggcga aatacggcac gctggccatt ctgcatcaca
    18721 ccgacgctgg ctggcagtat gtggcgggcg cgggtgcgca ggcgggcgat gcgttgtcgg
    18781 cgcatttctc ggcgggcgag gtggcgcggc tgaacacctt gttccaggcc aagaccggct
    18841 tcgccgatgt gcccatcggg cccgatggcc agtcgacgtt cgtggcgtgg cagggggtga
    18901 agaactggaa ctggctgatg gtgggcttcg gtgccaagtc ggctttcatg gcggcgagct
    18961 atcggtcgtt ggccctgcag atcggcatga ttctggtggg taccattctc atcgccgcgt
    19021 tgataggctg gctgtcggcg gccacgttgc atccggtgcg tgagatgatc gcgcacatgg
    19081 cgcgtttcgg gcgtggcgat ctcaccgccg acctgcccga ggtgccggcg cgctcccgca
    19141 atgaggtcca tgccttgttc ggcagtttgc ggcagatgca ggccagtctg ggtgaaacgg
    19201 tgtcgtcggt gcggcgcggg gtcgaggaaa tcgatgtggg ttcgcgtgaa atcgcggccg
    19261 gcaatacaga cctgagttcc cgcaccgagc agcaggccgc ctctctgcaa gagacggcgg
    19321 ccagcatgga acagttggcg gcgacggtgc gccagaacgc cgacaacgcg catcaggcga
    19381 acgggctcgc cagcagcgcg tccagtgtgg cggtgcgggg tggggaaatc gtcagccggg
    19441 tcgtcgacac catggcgggt atctcccagg ggtcgaagaa gatcgccgag atcgtcagtg
    19501 tgatcgaaag cattgccttc cagaccaata ttctggcttt gaacgcggcc gtcgaggccg
    19561 cccgcgcggg cgagcagggc aaagggttcg cggtggtcgc gagcgaggtg cgcagcttgg
    19621 cgtcgcgcag cgcccaggcc gccaaggaag tgaagatgct catcgatgaa tcgtcgagca
    19681 aggtggatga gggtgcccgc tatgtggagc aggctggcag caccatgacg gaaatcgtcg
    19741 cgtcggtgca gcgtgtcacc gacatcatgg gtgagatcgc ggcggcgtcc gatgaacagt
    19801 ccagcggcat tggccaggtg aatcaggcgg tgtcgcagat ggacgaggcc acgcaacaga
    19861 acgcggcgct ggtcgaacag gccgcagccg ccgcggcgtc cctgcaggag caggtgcaga
    19921 cgctgacgca ggccgtggcg gtgttccggc tgcgtggcgg ggcgcaggct caacccgtgg
    19981 gtcaggcggg cgactcgggc aggatgcccg gggggcggga agaggtcgaa cccacgacgc
    20041 atggtggccg cctgctgctg ccggccaccg gcgccgcttg agccgttcac caagccgccc
    20101 atggaggcgg catgcgggat tcacgctacc gacacgccga ttccaccttc gaccatggcc
    20161 ccgatcacgg tggcctggcc gaacccgtcc gcccgaagtt gcgccagcac ttggtccgcg
    20221 acgcctgccg cgcaactgac gagcaatcca ccggacgtct gcggatcggt caggagggtg
    20281 cgcgccacag cgggcaacct ggcatccaga tcgacctgtt ccccgtacga tgcccagttg
    20341 cgtcccgacg cgccggtgaa cacgcccgct tccgcgaagg cgcgcaccgg gctcagccag
    20401 ggtaggtcgt catggcgcag atgggcccgc aggccggcgc cccggcataa ttccagtgtg
    20461 tggcccagca ggccgaaccc cgtcacgtcg gtcatggcgt ggacgccagg caggcgcgcc
    20521 agcgccgggc cggaacggtt gagctgcgtg gttgacgcga tcatctgcgc atagccatct
    20581 tcgtcgagca gattcttctt gagcgccgcc gacagaatac ccacacccag tggcttgctc
    20641 agaatgagcg cgtcgccagc ctgcgcatcc tgattgcgcc ggatgtggtc cgggtgggcc
    20701 acgcccagcg ccgccaggcc gtagatgggt tcgacggaat cgatggaatg cccgcccgcg
    20761 atggggatgc cggcctcggc gcacagcgag gcgccacctt ccaggatgcg cgcgatcgtt
    20821 tcatgcggca gcacgttgat gggcatgccc accagggcca gcgccaggat gggcgtgccg
    20881 cccatggcgt agatgtccga cagcgcgttg gcggcggcga tgcgcccgaa gtcgtacgga
    20941 tcgtcgacga tgggcatgaa gaaatcggtg gtggcgatga tggcctgctc gtcgttcagg
    21001 cggtagaccg ccgcgtcgtc ggatgtttcg ggtcccacca tcaggcccgg aaacgcctgg
    21061 gtccggggca ggttcttcag caattcggcc agcacaccgg gggaaatctt gcagccgcag
    21121 ccgccgccgt gggacagttc ggtcaggcgg ggagggtggg cggtggtcat cagatgtgct
    21181 ccgtcaggcc cgacacgcgg gccagcaatg cgtcagcctg gccgcgggtg tcctcggcgc
    21241 aggggtggaa ttcaaatgcc agggcgccgg acatgtcgcc gaagctgcgt gaggtgctgc
    21301 gcgcgtaggc cgggtcatag tgacgggtga tcagatcgcg gaatagttcg ggtgcggcgt
    21361 cggcgtcgat caacccgtgc cagcgggcga tggtgtcgtt gccgtgcaga acggccaacc
    21421 ggccgagttg ctgcttgaac gtggctttgt cgctgaacag gtgcgcgtag tcctcgagca
    21481 ggaagccaat gcgttcggcc agcggggttt ggacatccac gcaggcgccg cggcggtagc
    21541 tgtccagcat cgccgtgggc aacgtgatct ggccgatgcg acggctctcc gcctccacga
    21601 aaaccgggcg gccggggtcg aaggtggaca agacctgggc cagtgccgac tcgaacgttt
    21661 tttgtgatgg ctgatcctgc cccggccagg cccccagcag cgacccgcga tgccgtgaca
    21721 ggccttccag gtcgagcgtc tgggcgccag cctgggccag ggcgtgcagc aggcgggtct
    21781 tgcccgagcc cgtgctgccg gtcagcacca cgtagtggaa ctgacccggg aggtcatcca
    21841 ggcgttgcag cacgtggcgg cggtaggtct tgtagccgcc ttccagctgg cgcgcgcgcc
    21901 agcctatcag gttgaaccac gccgtcatcg agccggagcg tttgccgccc cgccaacagt
    21961 agacgagggg gcgccattgc cgtggccggt cggcgaaaag cgtgtccaga tgccgggcga
    22021 tgttgtgggc gaccatggcg gcaccacggc gcgtcgcgtc gaaaggcgcg gtcttgtaca
    22081 gggtgccgat ctccgcgcgt tgcgcgttgt cgagcaccgg ggcgttgagg gcaccgggaa
    22141 tgtgatcgtc ttcgaattcg gccggcgtgc gcacatcgac gatctcgtcg aactcggcaa
    22201 gctgttccag attggctagg taggggatca cggtggtgct cgttggtggt tgtgtggaag
    22261 tgtacgacag cccctggtgt cgggccaatt tgcgggcgtt tgggtcgcgc cggtacaatg
    22321 cagggcgcat tgccgcgttc ttggcggcgc tctcgcggcg gcctgccgcg ccccgtccag
    22381 gcgggcgaca aaattcatca cccagggcct tcctcgcaat gacaaccatt ctcgaacacc
    22441 ttcccgtcgg ccagaaggtc ggcatcgcat tttccggagg cctggacacc agcgccgcgt
    22501 tgctgtggat gcgcaacaag ggggccgtgc cctgcgccta tacggccaac ctgggccagc
    22561 ccgacgaatc cgattacgac agcattccga agcgcgcgat gcaatacggg gccgagaagg
    22621 cgcggttgat cgactgccgt gcacagctcg tcgccgaggg gatcgccgcg ctgcagtgtg
    22681 gcgcgttcca tgtgtccaca gcgggggtca cgtatttcaa caccacgccc atcgggcgca
    22741 ccgtgaccgg caccatgctg gtggcggcca tgaaggaaga caacgtcaac atctggggcg
    22801 atggcagcac atacaagggc aacgatattg aacgtttcta ccgttatggc ctgctgacca
    22861 accccggtct gcaaatctac aaaccctggc tcgatcagca gttcatcgac gagctgggcg
    22921 ggcgcgccga aatgtcggaa tacatgcgcc aagccgggtt cgactacaag atgtcggccg
    22981 aaaaggccta ttccaccgac tcgaacatgc tgggcgccac gcacgaggcc aaggatctgg
    23041 agcacttgaa ttccggcatt cgcatcgtgc agcccatcat gggtgtcgcg ttctggcgcg
    23101 atgacgtgcc ggtcgcgcgc gaggaggtca ccatccggtt cgagcaaggc ctgcccgtgg
    23161 ccctcaatgg tgtggagtac cgcgacagcg tggagctcat gctggaagcc aaccgcatcg
    23221 gcgggcgcca tggcctgggc atgagcgacc agatcgagaa ccgcatcatc gaggccaaga
    23281 gccgtggcat ctacgaggct ccggggctgg ccttgttgtt catcgcatac gagcgcctgg
    23341 tcaccggcat ccacaacgaa gacaccatcg agcaataccg cgacaacggc cgcaagctgg
    23401 gccgtcttct gtaccagggg cgctggttcg attcccaggc catcatgttg cgcgagacgg
    23461 ctcagcgctg ggtggcgcgc gccatcaccg gcgaagtgac gctggaactg cgtcgcggca
    23521 atgattattc cctgctcgac acgcgctccc ccaacctgac gtacaagccg gaacgcctga
    23581 ccatggaaaa aggtgaatcc acgttctcgc cgcgcgatcg catcgggcaa ctgacgatgc
    23641 gcaacctgga catcaccgac acacgcgaca agctgttcgt ctatgtgaat gccgggttgc
    23701 tggcaccggc ggggaaggcg gcgctaccgc atctgaagga cgacagcgaa gagcagggca
    23761 agtaagggcg gggccgtgcg gcatgccgcc catttcgtgg ctccacgcct ctcattggtt
    23821 ggttgacacc agtttctgac ccgcaccccg gaagttgggc atcgatccaa ccttcggggt
    23881 gtttttcata ggcggacaga tttctttgta gccgtgtttt ccacaccggg gcggcgtcat
    23941 ggtgaatagc cgctttgtgc tgggttcagg cggacggggt gtccaactgc cccagcagca
    24001 ggaattccat cagggccttc tggacatgca ggcggttttc cgcttcgtcc cagaccacgc
    24061 tttgggggcc gtctatgacc tcgcccgtga cttcctcgcc gcgatgggcg ggcaggcagt
    24121 gcatgaatac cgcgtcgggc gcggcgacgg ccatcatgtc ggcgtcgacg caccagtcgg
    24181 cgaaagcctt gcgccgggcg tcgttttcgg cctcgtaccc catgctggtc cagacatcgg
    24241 tggtgaccag atgggcgccc cggcaggctt gcatgggatc gtcgaactcc tggagcgcgt
    24301 cggcggcggg ttcgcccacg cgttgtggat cgagccggta gccttcgggg gccgaaacgt
    24361 gcaggcggaa gcccagcatt tcggcggcct gcagccacgt gtaagccatg ttgttggcat
    24421 cgcccaccca ggcgacgatc ttgccttcga tggagccagc gtgctcgatg aacgtgaaga
    24481 tgtcggccag aatctggcag ggatggaatt cgttggtcag gccgttgatg acgggcacgc
    24541 gcgagtgtgc cgcgaagcgt tccagccggg tctgttcgaa cgtgcggatc atgaccacgt
    24601 cgaccatgcg tgaaatgaca cgcgccgtgt cttcgatggg ctcggcgcgg cccagttggg
    24661 aatcacccgt ggtcaggtgt atgaccgacc cgcccagttg gtacatgccg gcctcgaacg
    24721 acacgcgggt acgggtgctg gctttctcga acaccatggc cagcgtgcgg tcgtgcaggg
    24781 tgtggtgcgg ttcgtaatgc ttgaatttgt tcttgatgag gcgcgcgcgc tcgagcacat
    24841 aaaggatctc ggccttggtg aaatcactga attgcagaaa gtgtctgagg gggccggttt
    24901 gcaaggcggc gttggacatg gtgcgctctg ggtaaaacga taatcttacc ttgcggcccg
    24961 acggcgtgca aagctctgcg cgctccgcgg cgtgtttcgg gattatgttt cgaggttttc
    25021 agcgacggtg gcggatcgga tacaatgcgg cgcgggcaag gggtttgagc cgccgcttcc
    25081 ggcggactcc atccgtggaa gttcaatgac acatatcgtg cagcaaacca tcattcatgg
    25141 cacgacgctg gacggccagc gtttccgtcc cagtgactgg gccgagcggt tggccggcgt
    25201 catgtcgcag ttccgacccg ctggatgccc tggcgggcgt ctttcctatt cgccgtacgt
    25261 cgtgcccacc ttgctggacg gcgtccgttg cgtcgtggtc gatgggcggc tgcgtgacct
    25321 ggagcctctg gcctggaagt tcgtcatgga tttcgccagc gataaccatc tgcggacgtc
    25381 cgaacgggag ttcgatgccg cgtcggatga ttgggggggc acgcgcccca gccccctggc
    25441 ggactgagac gatttcgggg tggcgactcg gttatccaaa attggctgcg tgtccagggt
    25501 ccgaaaccca aaacccaaaa ccggtgtcgt gccaccccag ggctttcagc tcgatcgatg
    25561 tggccgtccg ggccaatggg gtgggccaag tgcgatcagc caagcacgct gagcaaaagc
    25621 gcggagaaca atgcacaaag tgaacgcaca aagtaaaaca ggacgccagc gcgtcctgtt
    25681 gcggcattcc agcagaaatc gctggaaagc gtggccggcc ctataaccgg ccagtgggtg
    25741 tttagcccag cgccttgatg gcggcggcaa ggcggctctt atggcgagcg gccttgtttt
    25801 tgtggatgat ctttttgtcg gcgatgcggt cgatggtgcc ggtggccttc ttgaagacat
    25861 cggcggcgac ggtcttgtca ccggcttcga tggcctggcg cacgcgcttg atggaagtgc
    25921 gcagcatgga acgcaggctg gcgttgtgtt tgttgcgctc caccgattgg cgggcgcgct
    25981 tgcgagcttg ggcagagttg gccatagtat gaaaagatcc gctgatgtgt cgtactgttg
    26041 tgtggtgcga tgtgtcgtgc agctgtatca atgccgtttg ccacgccgtg agtccgccct
    26101 gtgtcgtaac cccgtgtgtc gtaacctaat gagtcgttgc acttggcccc tgcttatctg
    26161 tgcaagaacg tttgcaaaag cccccaaggc ccgttcaatt tcggctgaaa cggcctgccc
    26221 actctttttt caaacccccg gctttcaggc cgggctaaga ataggcaaat tgacaaagac
    26281 agctattcta tattaacggc cgcgccgtgt aaagtcacgg gggcgaaacc cacacacaaa
    26341 cagcacggtg aaatatacga tcacgctggc ggctagcacc gcgagcagcc atagcccccg
    26401 ctgcccgcca tggtgggtgg ctcccagttg aagccagtcg atgcgctggt cggccacaat
    26461 cagcagggcg ctgagcgcgg ccagggcggg aatgatgcgc aggcggaagc gccgccagcc
    26521 cgggccgggg cgatagatgc cgcgtcggcg cagcatcacc agcaggcaca gggcgttgac
    26581 gctggctccc agcccgacgg acaaggccag accggcgtgg gcgaaccgtg gcacgaatat
    26641 gacattgaac aactgcgtga gaataagcac gaccacggcg atcttgaccg gggtgcggat
    26701 atcctgcctg gcgtagaagc ccggagccag tatcttgatg gacagtagcc cgacgatgcc
    26761 cacggaatag gccatgaccg ccaggcgggt ctccaggacg tcgtgggcgt tgaaggcccc
    26821 gtagttgaac agcgtggcca ctagcccttt cgatagcagg gcaagaccca gcgcggccgg
    26881 caagcccagc agcagcatca gccgcaggcc ccagtccagc agttcgctgt agccggtgtg
    26941 atcctggcgc gcatgggcgg ccgacaggac cggtaggagc acggtgccca gcgccacgcc
    27001 cagcagcgcg gtggggaatt ccatgagccg gtcggcgaac gacaaccagg tgacgctgcc
    27061 gggcgtcagc caggtggcga tgttcgtgtt gatcaacagc gaaatctggg ccacggacac
    27121 acccaggatg gccggcatca tctgatgcat gatgcgtttg accatggggt cgcgccaggc
    27181 ttgacgcacg cgcggcgaat agcgcggcgc caagcccagg cgcgagatgg cgatccactg
    27241 cacgaccagt tgcgcggcac cgccgatcat gacgcccgcc gcgagggcgt agatgggaac
    27301 gtcgaagtgc cgcgtcagga agaggcacgc gccgatcatc gacagattca gcagcaccgg
    27361 cgtgaaggcc ggcacggcga accggctcca ggtgttcagc acgcccgacg cgaaggcgat
    27421 gagcgacatg aacacgatgt aggggaacat gacccgggtc atccacacgg cggcgtggaa
    27481 ttcggcatgg cgggtcgcgc cgcgcaggcc gctggccatc agtgtgacga cccagggggc
    27541 cgcgatgacg ccgatcacgg tgaccagcat caggatggcc gtgagcagca gggccacgcg
    27601 atccagcaag atgcggaccc cgtcgtgcga attctccagg cgtgcctggc ccagtatggg
    27661 aacgaacgct tgggagaacg caccctcggc gaacaggcgc cgcaagaggt tcggaatgcg
    27721 gaaggcgacc cagaacgcgt cggtgagcgg acccgctccg aacgtgctgg cgatcaggat
    27781 gtcgcgcagc aggccggtga tgcgcgatac aagagtgaaa ccgctgaccg tggccgtgga
    27841 gcggagcagg ctcatcgatg cggacggaag gggctacttc ggcgccatgc gtatcgcacc
    27901 atccagccgt atggtttcac cgttgagcat gctgttgcgg atgatctggt tgaccaggct
    27961 ggcgtagtct tcgggggtgc ccaggcgggg tgggaaggga atgctggcgg cgagcgagtc
    28021 ctggacttcc tgcggcatgc cgaagatcat gggcgtgccg aagatgcccg gagcgatggt
    28081 catgcagcgg atgcccagtt tcgagagatc gcgcgccagc ggaagggtca tgccggccac
    28141 gccggccttg gacgacgcat aggctgcctg gccgatctgg ccatcgaacg ccgccaccga
    28201 ggcggtgtta atcagtacac cgcgctcgcc tgtggattcg ggttcgttgc ggctcatggc
    28261 gtcggctgcc aggcgacaca tgttgaaagt gccgatgagg ttgatgccca cgaccttgcg
    28321 gaagagatcc agcgaatgtg gcccgttctt gcccacggtg cgcgaggccg gcgcgacgcc
    28381 ggcgcaattg actaggccga acagcgggcc ggcggccagg gctgcggcga cgaccgcctg
    28441 cccgtcggct tcctgggtca cgtcgcaatg cacgtaggcc tggcccagtt cggcggccag
    28501 tttctgtccc gcctcgtcct ggatgtcggc caggacgacc ttggcgcctt cggcgaccag
    28561 catgcgaacc gtgccggcac ccaggccgga cgccccaccg gtgacgataa agactttgtt
    28621 gcggatttcc atggattgtt ccgagggttg ctgtctcgcg ggcgccgacg gcgcgcgaga
    28681 cggatggttc gggatggtca ggcggcgacg gacagcgcct ggaagatacg ttccttgatc
    28741 tcgtccaccg agccgagacc cgacagtttg cggtaggcgg gagcgtcggc ggcgccagtg
    28801 gcggcccagc cggagtaata gtccaccagc gggcgtgtct gcttgcgata cacggccagg
    28861 cggtgcctga cggtttcttc cttgtcgtcg tcgcgctgca ccaggggttc gcctgtcacg
    28921 tcgtcaatgc cgggggtctt ggggggattg aatttgacgt ggtaagaccg cccgctggcg
    28981 gggtgcgtgc gccgcccgct catgcgttcg atgatgttgt cttcggggac gtcgatctcg
    29041 agcacgaaat ccagagcgat gcgcgcgtct ttcagggcgt cggcctgtgg gatggtgcgg
    29101 gggaacccgt cgaacaggta gcccggctca cagtcgggtt gccgcaggcg gtcacgcacc
    29161 agaccgatga tgatgtcgtc ggaaaccagg ccacccgtat ccatgatctt cttggcttcc
    29221 agtcccaggg gggtttgcgc cttgaccgcc gcgcgcagca tgtcaccggt cgagatctgt
    29281 ggaatgccga agtgctgggt gatgaacgtg gcttgggtgc ctttgcccgc gccgggcgga
    29341 ccgagcagaa tgagtcgcat gaactattcc tcctggatgt cgtttttttg ctttcccgat
    29401 tatgccgcaa cgcagcatcc cgtgcatagg cgggagatgc ctggatgcat gacggcttgc
    29461 acgtgtcagg gcgcgttggc gaacacttgc cgcacgcgcg tcagatccgc ctcggtatcc
    29521 acgccggccg ccggtgggga gtcggtgagg ccgaccatta tgacatagcc gttttccagg
    29581 gcgcggagct gctccagtga ttcgaacgtt tcgagcgagc cgacgggcaa tgacgggaag
    29641 cgtttcagga agccggcgcg ataggcgtac aggccgatgt gatgcagcgc cggcaatccg
    29701 gcggccaggt gtcgttcgcc gtccgccaac gcgtcgcggg cccacgggat gggggcgcgg
    29761 gagaagtaca gggcgcggcc ccggtggtcg cgcacgacct tcaccgcgtt cgggttgaac
    29821 aacgcttgcg catcggtgat gggcgccgcg caggtggcga tggcggcttc gggattgtcg
    29881 ccgagcaggt tcgccacggc gttgatctgg gccgggtcga tcaggggttc gtcgccttgc
    29941 acattgacga cgatggtgtc gtcggtaagc gccagggctt cggcggcctc ggcgagccgg
    30001 tcggtccccg tggggtgctg ggtgcgggtg agcagggctt cgaatccgtg gtgccgcacc
    30061 gccgcctgaa tatcggagga gtccgttgcg accaccacgc gactggccag ggagcggctg
    30121 gcctgttcgg cggtgcgcac caccatgggt ttgccggcga tgtccagcag aggctttccc
    30181 ggcaggcggg tggaggccgc ccgcgcggga atgatggcga tgaagctcat gctcgctggt
    30241 cgtccgtggc ggcgccggtg gccggcccgg acggcgcgga gtgaagatcg cgggcttcct
    30301 gttcgagcat gacggggatg ccgtctcgaa tggggaaggc cagtttgtct gtgttgcaga
    30361 taagctcctg ttgctcgcgg ttgaagtgca acgggccctt gcacagcggg cagaccagga
    30421 tttcgagcag gcgggattcc atgcgggact ccggaaaatg agatgactga acagctatca
    30481 agtgtaatgc gccgacgctc ccggggtctc tgaacgggag tccttgtttt cgacgatgtc
    30541 gcgcagtcgg cgcgcgatgt catccagcca gtgcgaatcg gtgaagcgcg gcgtggcgtg
    30601 caccacccac agacggtcat cgttggtgaa agcgccgcat ttgatcgcat ccttggcggt
    30661 gatcaggatg gggccggggc ccagcgcgtc gaagggcgag gtctggaatg cggcgtgatc
    30721 gggcagcgcg acggtatgcg ccagcgcgag tccgcacgcc tcgagcatgc cgaagaagcg
    30781 ctcgggtcgg ccgataccag ccacggcatt cgccggggtt cggccatgtt cgatgagcca
    30841 ggtgctccaa tcgacgctgc gtcgcgtggc cagatgctcc acgtggctcg gaaacaggcg
    30901 catgcccaca gtcggtacgc ccccggtcga ggcggggtct gattggcccg gttcgaggtt
    30961 gacgaccaag tagtcgatct ggcccagtct gtcagcgggc tcgcgcaggg ggcccgcggg
    31021 caacaggcgg ccgttgccga taccgcgggc atcctgcacc gccagttcga tatcccgacc
    31081 cagctcgatg tgctgcagtc cgtcatccgc aatgacgacg tcgatctccg ggtgcgcggc
    31141 ttcgagtgcg cgcagggcca ggatgcggcg cggatgcacg cccaccggca caccggttcg
    31201 cagagcgatc agcgcgggtt cgtcgccgaa ccgcgcggga tccaggtggc cggtgccaac
    31261 gcgtggcgcc tggctcgagg ccacgccgta acctcggctg atcacgccag gggtccagcc
    31321 gcgttcccgc agcccttgcg ccagcgcgat gacgacaggc gtcttgccgg tgccacccac
    31381 gtagatgttg ccgaccacca cgactggaga acggccgcgc tgccgagcat ccggccgggc
    31441 gaatcgttgg cgcttgtgcg ccacgacaag ccgtgtgatc catgaaaacg gcagcaggac
    31501 tgtgctggcc agtcctttgc cgcgccacac gcggtgggct agcgtggcca gtcgcgcgga
    31561 cggcttcaag gcgtgctttg cgtggcgaag tccacgcgtt ctatgcctgc ttggcgcgcc
    31621 gccagcatgg cccggaccac cgcttcgtgg gtggactggg cgtcggcgtc gatgatgagc
    31681 gtgccttgcc ggcgctgggc agcctcggcc ttcagtgcct gggccagatc gtcaacgctg
    31741 gttccttgaa cgtatcgtcc gtcgagcgca tatacgcccg tctgactgat ggcgaggttc
    31801 atggcctgtg gctgcggcgc gtcggcgctg gcctgcggca gatgaacctt gatctggctg
    31861 aaacgtgtga acgacgtggt ggcggccaaa aaaataagaa tcaccaacag cacgtcgatc
    31921 agcgggacca gattgatctc gggctcgtca tcggtgcggg agcggcggaa gttcattgcg
    31981 gtgtgcggct gatgtaccgg ttgagcgcgc tggcttcgcg ttccatctga tgcaggaaat
    32041 aatccacccg cgagcggaaa tagcgatgga agatgagcgc ggggatggcg atgagtatgc
    32101 cgaacccggt gttgtacagg gcgatggaga tgccgcgggc gagttgtgac ggatcaccgc
    32161 ccgcgggcgt gtacgcggcg aagatctcga tcatgccgac gaccgtgcca aacagtccca
    32221 tcaatggtgc caccaccgcg atggtggcga gcgccggaag gtatcggttg agccgatggg
    32281 cgacgtcttt gccggcgtct tccacagcct cgacccgcaa cgcgccgctc tcttggcgat
    32341 ggatgagcac ctcggccagc acctggccca gaggggaact ggacgccagt cgatcgagcg
    32401 tttcaggggt atctgtgccc ttcttgacga ggtcggcgac ctgtgctggc aggcgcgcgg
    32461 gcagcaccag cgcgctgcgc agggacagaa aacgctcgat gatgacggcg aggcccagca
    32521 ccgacgtggc caccaacagc catatcggcc aacccgccac acgtaggatt tctagcaact
    32581 gtgtctcccg agagctgaaa gcgccaagac caacgaacaa tcatccaggc aaatcatcga
    32641 ttcaacccgc attttaaagg ctggccggcc tcctgacgac aacacccccg ggggaagggt
    32701 cgccctcgtt ccgtccgcgg tcggttccgc cctatgccaa aataggcgga cagtccgttt
    32761 tccagccctg ccggccctga ttcagcgtcg gcaggctttt ccgcagagag ttcctatgtt
    32821 tctcaagaat gcctggtatg tcgccgccct cgggtccgag gtcggccgtt cgctgctggc
    32881 cgtccggttg ctgggcgagg acatcgtcct gtaccgcaag ctcgatggga aggtggcggc
    32941 attggaagac gcctgtccgc atcgcaagct gccactgtcg atggggcgcc tgaaagaaga
    33001 cacagtcgaa tgcgggtatc acggcctggt gttcgattgc agtggccagt gtgtgcacgc
    33061 gccaggtctg gcgcgggcgc cgcgcgcggc ggtggcccgc gcctatcccg tcgaggaacg
    33121 ctacggcatg gcctgggtct ggatgggcga tcccgcgcgg gccaatccgg acagcattgt
    33181 cgaggtcgac cattggggcg acccggcctg gggtgtgaac cggggcgatg ccatgaccgt
    33241 gcaatgccac tatctttata tcaccgacaa cctgctcgat ccgtcacacg tgtcgtgggt
    33301 gcaccagact tcgttcggca acgcggacat cattggcgag cctctggaaa ccgacgtgcg
    33361 tgaagagggc gtgcgggtgt cgcgctggat gcgcgacgtc gaggtcgcgc cgttctatgc
    33421 gaaattcgtc cggttcaagg ggcattgcga ccgcaagcag cactacgagg tccgtttccc
    33481 gtcgcaggca gtcattcggg cgatcttcac gcccgccggc acggcgaccg actcggaccc
    33541 gttccacccc gacgtattcc tcatggattc gtacaacttt ctttcgcccg tggatgagtc
    33601 cacgacgcgc tatttctggt ttcagatgcg taatttcagt cccgatgacg aaggcgtttc
    33661 gcgccagttc tctgatgacg tgcggcatgc cttcgaagaa gaccgcgccg tgctcgaggc
    33721 cgtccatcgc ggcatggcga gaagcccttc ccgcatgaat ctgcccctgg attccgggcc
    33781 gctccggttc cgtcgcaagc tgggcgagat gatcgatgcg gaactggccg aggccggcct
    33841 ggaggcggat gtcgacatgt cgggcccccg tctgtacacg acctgatcgc gaatcgggtg
    33901 tcgagcctat tgcaaccttc aagtgatggc ggtcgaatgc ccgatgtgaa acaggctgtt
    33961 cggtatgatc gattgcgtga caatggcttg cgctgtctca atttcgagca tataaagcgc
    34021 tggaaaatga gacatggcgt cggatttcga acaagaatgg cgtgatgcct tgcgctcccg
    34081 agggtatcca caaattctgt ggataattct gtgggtaaac gtgccggcag gcttgattcc
    34141 atagggctgg aggttcaatg gtcaatctga acccatcatg tcatgatctt aaatatcttt
    34201 attaatcata ttgttagcga tttaaattga agtgcgagca catcttttaa agcgattggc
    34261 ttgatatttc agtaatttga caggcggatt tttcctgtgg aaataccaag gggggaatgt
    34321 gatcatcgat aattccgaag ccgtgttgac ggtgtcagag ctgaatcgtc gcgtcgggcg
    34381 gttgctcgaa ggtgaattcc ccaaagtttg ggtcaaaggc gagatatcca atttcacgcg
    34441 agcggcctcg gggcactggt atttcaccgt gaaggacgag ggtgccgccg tgcgcgcggt
    34501 catgttccgt ggccgggccg ccagcgtcgg attcgttccc aaggcgggtg agcggttcga
    34561 gttccgcggc tccgtgaccc tgtatgaacc gcgtggtgat tatcaacttc aggtcgacat
    34621 gctgcgtcgc gcgggtctgg gaaacctcca cgaggcgttc gtgctgttga aaaaccggtt
    34681 ggccgacgaa ggattattcg atggcgagcg gaagcgcccc atccccgcga tgccgtcggc
    34741 catcggagtg gtcacgtcac tggccgcggc ggcgctgcac gatgtgctgt cggtgctggc
    34801 gcgccgtgtg ccgcatgttc ccgtgatctt gtatccggcc cccgtgcagg gggcggatgc
    34861 ggctgcgagg ctcgttcagg cagtcgacgc cgccgtgcgg cgggacgagg tcgatgtgct
    34921 gcttctggtg cgcggcgggg gcagtctgga ggatctctgg agtttcaacg atgaggggtt
    34981 ggctcgcgcg ctggcggcct gccccattcc catcatcagc ggtgtggggc atgaaaccga
    35041 cttcaccatc gctgattttg tcgccgatct ccgcgcaccg acgccgaccg cggcggcgga
    35101 actctcatgt cagtcgcgcg acgattgcct ggcattggtt cgacgtgccc tcgatgcctt
    35161 gacgcggcgg caacggcgcc atcttgacac cgcggcattg cggctggatc gggcggcgtc
    35221 cgcgctggtg tcgcccgcgc agcgcgtggc gcgccagcgc gagagcctgg agtccctggg
    35281 tgctcgcctg gcccgggcgg ccgctctggc gatggaacgc tgcggcgttc gtctcgatca
    35341 tgcacgcgcg cgcatgcggc gatccgggcc cgatacccgc cgtgcgcgac aagacctcgc
    35401 ccgttgcctg gagaggctgg gcgcggcggg gggtaatctg gtgccacgcc gtctcgatgc
    35461 cttgtcgggg agtgtccaga cgctgcaggc gctggatccc cggcgggtgc tcgggcgtgg
    35521 ctatgcgctg gtccggaacc gcgacgggga gctcgtcaaa aatgcgctag acttgaacat
    35581 cgacgaacga ttgaaccttg agttcggaca aggccgcgcc gaggtctcgg tggtgtccgc
    35641 gcaaggttcg acctccgctg acaagtgccc ctgatcacgc cggaaacccg gttctccggg
    35701 ctgcgaacag ggcacgcgca ccctttggtt ttctgctttt cgcgctgttt ggtggcttat
    35761 acaataggcg tgctccgttg gtttttttag aaggaatatg acatggcatt tacgcttccc
    35821 cccctgcctt acgacatgga tgctctggcg ccgcacatct cgaaagagac gctggaattc
    35881 cactacggca agcatcatca gtcctatgtg accaatctca acaacatggt gccgggcacc
    35941 gagttcgaat cggcttcgct cgagcaaatc atcaagaagg cctctggtgg cctgttcaac
    36001 aacgcggcgc aggtatggaa ccacacgttc tactggaata gtctttcccc caaaggcggc
    36061 ggtgagccca agggtgcgtt gctggccgcg atcaacgcga aatggtccag cgtcgccgct
    36121 ttcaaggaag ccttcagcaa gtcggcggct ggcaatttcg gttccggctg gacctggctg
    36181 gtcaagaaac ccgatggcag ccttgatatc gtcaatacca gcaacgcggc cacgcccatc
    36241 acgggtgccg acgtccccct gctgacctgt gatgtctggg agcacgccta ctatatcgac
    36301 taccgcaacg ctcgtccgaa gtacctcgag catttctgga atctcgtcaa ctgggatttc
    36361 gccgccaaga atctgggctg atcccgtctc gcaccaggtc gggcaagaac gccccgggcg
    36421 gttcgagagc cgcccggggc gtttccctgg ccgaggtgcg ggccatcgtg ttggcccgtg
    36481 ccattgcttt ggttcgtgtc ccgttccggg cgcgaatcca ttcctcggtt tcgtgcatcc
    36541 gcttgcacgg cggcgcctgg gccagccttg cggcgcgtga acactggata cctctcatca
    36601 tgacgaccca agcttatatt tgcgatgcca tccgcactcc gtttggccgg tacggtggtt
    36661 cacttgccca ggtgcgcgcc gatgatctcg ccgcggcccc catcaaggcc ctgatggcgc
    36721 gcaaccctgg tgtcgactgg tcgcgcct
//
LOCUS       DASTNK010000028         7557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_386256, whole genome shotgun sequence.
ACCESSION   DASTNK010000028 DASTNK010000000
VERSION     DASTNK010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7557
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386256"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..587
                     /locus_tag="VFR20_02225"
     CDS             39..587
                     /locus_tag="VFR20_02225"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEU0229364.1"
                     /translation="MKTVGDKLEPFKVTGVKPGFNQHEENGVSAFEDITESSFPGKWK
                     VIYFYPKDFTSVCPTEIVGFNKLEQEFADREAVLMGGSVDNEYVKLAWRREHPDLSKL
                     GHYQFGDTTGALVDQLGIRDKTEGVALRATFIVDPDNVIQHVSVNNFNVGRNPDEVLR
                     ILDGLLTDELCPCNRKIGGDTL"
     gene            complement(688..1176)
                     /gene="ispF"
                     /locus_tag="VFR20_02230"
     CDS             complement(688..1176)
                     /gene="ispF"
                     /locus_tag="VFR20_02230"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEU0229365.1"
                     /translation="MNIPFRIGQGFDVHALVAGRPLILGGVTIPHTHGLQGHSDADAL
                     LHALTDALLGAAGLGDIGRHFPDTDPAYRGADSRILLRHALGKVSAAGWVVGNVDATI
                     HAQAPKMAPHIPAMVANIAADLGLDAGQVNIKAKTSEGLGFLGRKEGIAADAVVLLVR
                     GQ"
     gene            complement(1187..2047)
                     /locus_tag="VFR20_02235"
     CDS             complement(1187..2047)
                     /locus_tag="VFR20_02235"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU0229366.1"
                     /translation="MTRKIIAIVPAAGVGERARAADSASAVDLAQDSAPPGGGGKASP
                     RPIGTVLPKQYGLLRGEPMLRWAVRALLVDDRVTEVRVVVAPGDGRAADALAGLPRTV
                     LRSCGGSTRAATVQAALEELTLDDDMWVLVHDAARPGLPAEDLRRLIDTCLAHGGGGL
                     LARRVADTVKQQAASTPEGNLIPDSHSARAARNPGKVLSSSPRADCDRVARTVRREGL
                     WLAQTPQMFPAQALLCAVRDARGAVDVTDEASAMEHAGQAPLLIPGSQRNFKVTWPED
                     FALMEKWLEP"
     gene            2076..5531
                     /gene="mfd"
                     /locus_tag="VFR20_02240"
     CDS             2076..5531
                     /gene="mfd"
                     /locus_tag="VFR20_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU0229367.1"
                     /translation="MSSETRASVSLPGTPSLLAALKPGQRYAVPAPPGSGDAWLLADL
                     ARTAGKPLVVFTADPLSAQRLADEIVLLAPELRVLQFPDWETLPYDSFSPHEDLISER
                     LRTLHALMREAVDVLTVPVTTALYRLAPPEFLAAYTFSFKQKDKLDEEQLRHQLALAN
                     YTHVTQVTAAGEFSIRGGLIDLFPMGSALPYRIDLFDDEIESIRSFDVDTQRSLYPVK
                     EVQMLPGREFPMDEAARNRFRAGFREVFEGDPSRATPYRDIGHGIAFAGIEYYLPLFF
                     EHTATLFDYLPSNTLAVTRGDIDAAMRQFGQDTHSRHQFLKSDRERPVLPPADLFLDD
                     QTFHVQLKTLARLALTLDGVHPDFLPPPDVAVARRADDPMAKLRALVSQDTGRTVLCA
                     DSAGRRETLLQMLSEFDLVPGSQPDSLRDGLQAPPGLSLTIAPLDAGFRIPALGLSIL
                     TENDLYPSQGGARRRTRRAQESSDVEAMVRDLSELREGDPVVHAQHGIGRYRGLIDMD
                     LGEGKMEFLHLEYATGSALYVPVAQLHVIARYSGADPEHAPLHQLGSGQWDKARRKAA
                     KQVRDAAAELLALYALRASREGYAFKLPPGDYQAFAEGFGFEETPDQAAAITAVIQDM
                     TSGKPMDRLVCGDVGFGKTEVALRAAFLAVLNGKQVALLCPTTLLAEQHAQTFADRFA
                     DWPVKVAELSRFRSSKEVDAAVQGLANGSVDIVIGTHKILSSSVKYKNLGLVIIDEEH
                     RFGVRQKETLKALRAEVDVLTLTATPIPRTLGMSLEGIRDFSVIATAPQRRLAIKTFV
                     RREDGSTIREALLRELKRGGQAYFLHNEVETIHNRRARLEELVPEARVAVAHGQMPER
                     ELEDVMKGFYQQRYNVLLCTTIIETGIDVPTANTIIIQRADRLGLAQLHQLRGRVGRS
                     HHQAYAYLLTPGEDAITANAKKRLEAIQSMEELGSGFYLAMHDLEIRGTGEILGDSQS
                     GNIHEVGFSMYTDMLDTAVRALKAGEEPDLDTPFAASCEVNLHASSLLPSDYCPDVHA
                     RLQLYKRLSHASNEDALIGIQEELIDRYGKLPEAAQNLLATHRLRIRAEPMGIVKIDA
                     SESQAMIQFSAKPKVDPMRVIQLVQTQKHVKLAGQDRLKIALAQGAQLTARTDAIRQV
                     LRALG"
     gene            5582..6433
                     /gene="serB"
                     /locus_tag="VFR20_02245"
     CDS             5582..6433
                     /gene="serB"
                     /locus_tag="VFR20_02245"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEU0229368.1"
                     /translation="MTHLILQAPNLNHAIIENLAAELEAAGVQHLGPTAVRLLDVDGS
                     RRTEIGGFCDEHGMDHAFLDKVDRLADCRILASDMDSTLINIECIDEIADMAGKKAEV
                     SAITEAAMRGEIADFSESLRRRVGLLRGVPASTLDRVYQERLRLNPGAEQLVAAMNRH
                     GVKVMLVSGGFTFFTSRLAQRLGLYSSHANELEIVDGKLTGNVLGPVVDGQAKAAFLR
                     ELAGELGALPGQIIAMGDGANDLPMLSLAGYSVAYRAKPVVRKQARFALNVSGLDAVL
                     NWFRRNH"
     gene            complement(7222..>7557)
                     /locus_tag="VFR20_02250"
     CDS             complement(7222..>7557)
                     /locus_tag="VFR20_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229369.1"
                     /translation="AIRALPKSRRTCRNATFRPGAISGNQNIMKSFRLTLRLVFIAVA
                     CASVAACADNPSRQTDLDYQSQPSLTMQGVSSSTGSSGQRLTIQSGQGEELNLPWFIR
                     DAQEWINNN"
BASE COUNT         1460 a         2506 c         2278 g         1313 t
ORIGIN      
        1 cggtgcgatt ccccccttcc caagagacag gagcattcat gaagactgtt ggcgacaagc
       61 tggaaccctt caaggtgacc ggcgtgaaac cgggcttcaa ccagcacgag gaaaacggcg
      121 tatcggcgtt cgaagacatc accgaaagct cgtttcccgg aaaatggaaa gtcatctact
      181 tctacccgaa ggacttcacc tcggtctgcc ccacggaaat cgtcggtttc aataaactgg
      241 aacaggaatt cgccgaccgt gaggccgtat tgatgggggg gtcggtcgac aatgaatacg
      301 tgaaactggc ctggcgccgc gaacatcccg acctgagcaa actgggccac taccagttcg
      361 gcgacaccac gggcgcgctg gtcgaccaac tgggcatacg cgacaaaacc gaaggcgtcg
      421 ccctgcgcgc gaccttcatc gtcgatccgg acaacgtcat ccagcatgtg tccgtcaaca
      481 acttcaacgt cggccgcaac cctgacgaag tcctgcgcat cctggacggc ctgctgaccg
      541 acgaactctg cccctgcaac cgcaagatcg gcggcgatac gctgtagggc tcaggaaatc
      601 cgccaaacgc tgcccacggc tcatacagga cgggcttcgg ccagaaccgc caccctgatt
      661 tcatctggca aggcattggg cgtgacgtta ctggccacgc accagcaaca cgacggcgtc
      721 ggcggcgatg ccttccttgc gcccaaggaa ccccaggccc tcgctggtct tggccttgat
      781 gttcacctgc ccggcatcca ggcccagatc cgccgcgatg ttcgcgacca tggcgggtat
      841 atgcggcgcc atcttgggcg cctgggcgtg gatagtggcg tcgacgttgc cgaccaccca
      901 acccgccgca ctcaccttgc ccagcgcgtg gcgcaatagg atgcggctgt cggcgccccg
      961 gtatgcggga tcggtgtccg gaaaatgacg accaatgtca cccaggcccg ccgcgcccaa
     1021 caaggcatcg gtcagcgcgt gcaacagggc atcggcatcg gaatgcccct gtagcccgtg
     1081 ggtatggggg atagtgacgc cgccaaggat cagcgggcgc ccggcgacca gcgcatggac
     1141 gtcgaagccc tggcctatgc gaaacgggat attcattggg taatccttac ggttccagcc
     1201 atttttccat caacgcgaag tcttcgggcc atgtgacctt gaaattgcgc tgggagccgg
     1261 gtatcaatag tggcgcttgc ccggcatgct ccatcgccga tgcctcgtcg gtgacgtcca
     1321 ccgcaccccg tgcatcacgc acggcgcaca agagcgcctg cgccgggaac atttgcggcg
     1381 tttgagccag ccataggccc tcgcgccgca cggtgcgcgc caccctgtcg cagtcagcgc
     1441 ggggcgacga tgacaatacc ttgccggggt tccgcgccgc acgcgccgaa tgactatctg
     1501 gtatcagatt cccttccggt gtggacgccg cttgctgctt caccgtgtca gcgacccgcc
     1561 gcgccaacaa cccacccccg ccatgcgcca gacaggtatc gatcagccgg cgcaaatcct
     1621 cggcgggcag gcccgggcgc gcggcgtcgt gcaccagcac ccacatatca tcatcgagcg
     1681 taagttcttc cagggcggcc tgaaccgtgg cggcgcgcgt ggaacctccg caggaacgca
     1741 gcactgtgcg cggcaggcct gccagcgcat ccgctgcgcg gccatcaccg ggcgcgacga
     1801 ccacccgcac ttccgtgaca cggtcatcca ccaacagcgc acgcacggcc caacgcagca
     1861 tgggctcgcc gcgcagcaag ccgtactgct tgggcaacac cgtgccaatc ggcctgggcg
     1921 acgccttgcc gccccctccg ggcggggcac tatcctgagc aagatcgacg gcactcgccg
     1981 agtcagcggc tcgcgcacgc tcgcccaccc ccgcggcggg aacaatggca atgatttttc
     2041 gggtcatggg aaagaatttt ataatggtcg ccgaaatgtc ctcagaaaca cgcgcctcag
     2101 tgtccctccc cggtaccccc agccttctcg ccgcgctcaa acccggccag cgctacgccg
     2161 tgcccgcacc acccggatcg ggcgacgcct ggctgcttgc cgacctggcg cgcaccgccg
     2221 gcaagccact ggtggtgttc accgccgacc cgctcagcgc gcagcgcctg gccgacgaga
     2281 tcgtgctgct ggcgcccgaa ctgcgggtgt tgcagttccc cgattgggag actctgccgt
     2341 acgacagctt ctcgccgcat gaagacctga tttccgaaag actgcgcaca ctgcacgcgc
     2401 tcatgcgcga agccgtcgac gtgctgaccg tgccagtcac caccgcgctt taccgcctgg
     2461 cgccgcccga gttccttgcc gcctacacct tttccttcaa gcagaaggac aagctggacg
     2521 aagaacaact gcggcatcaa ttggccctgg cgaactacac gcatgtcacg caagtgacgg
     2581 cagccgggga attcagcatt cgcggcggac tgatcgactt gtttccgatg ggctcggcac
     2641 tgccctaccg catcgatctg ttcgacgacg agatcgaatc gatacgcagc ttcgacgtag
     2701 atacacaacg cagcctgtac ccggtcaagg aggtccagat gctgccgggc cgtgaattcc
     2761 ccatggacga ggctgcacgc aaccgcttcc gcgccgggtt ccgtgaagtc ttcgaaggag
     2821 acccgtcgcg cgccacgccc taccgggaca ttggccacgg catcgccttc gcgggcatcg
     2881 aatactacct gcccctgttt tttgaacaca cggccacgct gtttgactac ctgccgtcca
     2941 acacactggc ggtcacgcgc ggcgacatcg acgcggccat gcggcagttc ggccaggaca
     3001 cgcacagccg ccaccagttc ctgaagagcg atcgggaacg gcccgtcctg ccccccgctg
     3061 acttgttcct ggacgaccag acctttcatg tccagctcaa gacactggcc cggctggcgc
     3121 tgaccctcga cggcgtccac cccgatttcc tgccaccacc agacgtggcc gtcgcgcgcc
     3181 gcgccgacga ccccatggcg aagctgcgcg cgctggtctc acaggacacg gggcgcacgg
     3241 tgctatgcgc cgactcggcc ggccggcgcg aaaccctgct gcagatgctg agtgaattcg
     3301 acctggtgcc aggcagccag ccggattccc tgcgcgacgg cctgcaggcg ccgccgggac
     3361 tgtccctcac gatcgccccg ctggatgcgg gcttccgcat ccccgcgctc ggcctctcga
     3421 tactgacgga aaacgacctg taccccagcc agggcggcgc gcgccggcgc acccggcgcg
     3481 cgcaggaaag cagcgacgtc gaagccatgg tgcgcgatct gtccgagctg cgcgagggcg
     3541 atccagtggt gcacgcccag cacggcattg gccgctatcg cggcctgatc gacatggatc
     3601 tgggcgaagg caagatggaa ttcctgcacc tggaatacgc caccggcagc gcgctgtacg
     3661 tgcccgtggc ccagttgcac gtgatcgcgc gctacagcgg cgccgacccg gagcacgcgc
     3721 ctttgcacca attgggttcg ggccaatggg acaaggcccg gcgcaaggcg gcgaaacagg
     3781 tgcgggacgc cgccgccgaa ctgttggctc tgtacgcgtt gcgcgcctcg cgcgagggct
     3841 atgccttcaa gctgccgccg ggcgactacc aagccttcgc cgaaggcttc ggtttcgagg
     3901 aaacccccga ccaggcggcc gccatcacgg cggtcatcca ggacatgaca tccggcaaac
     3961 ccatggaccg gctggtgtgc ggcgatgtgg gcttcggcaa gaccgaggtc gcactgcgcg
     4021 ccgccttcct ggccgtgctc aacggcaagc aggtggcgct gctgtgcccc accaccctgc
     4081 tggctgaaca acacgcccag accttcgccg accgcttcgc cgattggccc gtgaaagtcg
     4141 ccgaactatc gcgattccgc tcctcgaaag aagtggacgc cgccgtacaa gggcttgcca
     4201 acggcagcgt cgacatcgtc atcggcacac acaagatcct gtcgagcagt gtgaagtaca
     4261 agaacctggg gctggtcatc atcgacgagg aacaccgctt tggcgtgcgc cagaaggaaa
     4321 ccctgaaagc gctgcgcgcc gaggtcgacg tcctcaccct caccgccacc cccatccccc
     4381 gcaccctggg catgtcgctg gagggcatcc gcgacttctc ggtgatcgcc accgcgccgc
     4441 agcgccgtct ggccatcaag accttcgtgc ggcgcgagga cggcagcacc atccgcgaag
     4501 ccctgctgcg cgaactgaaa cgcggcggcc aggcctactt cctgcacaac gaggtcgaga
     4561 ccatccacaa ccgacgcgcg cgcctggagg aactggtgcc cgaagcgcgc gtcgccgtgg
     4621 cccacggcca gatgcccgag cgcgagctcg aagacgtgat gaaaggcttc taccagcagc
     4681 gctacaacgt gctgctgtgc acgaccatca tcgagaccgg catcgacgtg cccacggcca
     4741 acaccatcat catccagcgc gccgaccgcc tggggctggc gcagctgcac cagttgcgcg
     4801 gccgcgtcgg gcgctcgcac caccaggcct acgcctacct gctgaccccc ggcgaggacg
     4861 ccatcaccgc caacgccaag aaacgcctgg aggccatcca gtccatggag gaactgggct
     4921 ccggcttcta cctggccatg cacgacctgg agatacgcgg caccggcgag atcctcggcg
     4981 attcgcagtc cggcaacatc cacgaggtgg ggttctcgat gtacaccgac atgctggaca
     5041 ccgccgtgcg cgccctgaaa gcgggcgaag aacccgacct ggacacgcca ttcgccgcgt
     5101 cgtgcgaagt gaacctgcac gcgtcatcgc tgctgccatc ggactactgc cccgacgtgc
     5161 acgcccggct gcagttgtac aagcggctgt cgcacgcctc gaacgaggac gccttgatcg
     5221 gtatccagga ggaactgatc gaccgctacg gcaaactgcc cgaggccgcg cagaacctgc
     5281 tcgccacgca caggctgcgg atccgtgccg aacccatggg catcgtcaag atcgacgcca
     5341 gcgaatcgca ggccatgatc cagttctcgg ccaagcccaa ggtggatccg atgcgtgtca
     5401 ttcaactggt gcaaactcag aagcacgtga aactggctgg ccaggaccgg cttaaaatcg
     5461 ccctggccca aggcgcgcag ttgacggctc gcaccgatgc gattcggcag gtgctgcggg
     5521 cgctggggta ggcttgcttt tcaacgccac tccaatcggc tctcaccacc acactccacc
     5581 catgacccat ttaatactcc aggcgcctaa cctgaatcac gccatcatcg aaaacctcgc
     5641 ggccgagttg gaagccgccg gcgtgcagca cctgggcccg accgccgtgc gcctgctgga
     5701 cgtggacggc tcccggcgca cggaaatcgg tggattctgc gacgagcacg gcatggacca
     5761 cgccttcctg gacaaggtgg accggctggc cgactgcagg atcctggcgt cggacatgga
     5821 ctcgaccctg atcaacatcg agtgcataga cgagatcgcc gacatggccg gcaagaaggc
     5881 cgaagtgtca gccatcaccg aagctgcgat gcgcggagag atcgcggatt tttctgaaag
     5941 cctgcggcgt cgtgtggggc tgttgcgtgg tgtgccggct tcgacgctgg atcgtgtgta
     6001 tcaggagcgg ttgcggctga accctggcgc tgaacagctt gtggcggcga tgaatcggca
     6061 tggtgtgaaa gtcatgctgg tgtcaggggg gttcacgttc ttcactagtc gattggcgca
     6121 acggctgggg ctgtacagta gtcatgccaa tgagctggaa attgtggatg ggaagctgac
     6181 cggcaatgtc ctggggcctg ttgtcgatgg gcaggcgaag gcagcgttcc tgcgggaact
     6241 ggcaggcgaa ctaggtgcct tgcctgggca gatcatcgcc atgggtgacg gggccaatga
     6301 tctgccgatg ttgtcgctag ctggttattc agttgcctac cgagccaaac cggtagtgcg
     6361 caagcaggcg aggttcgcgc tgaatgtcag cgggctggac gctgtgctga actggtttcg
     6421 tcggaatcat tgaagggcgt tctgatttga cgtcgtcctt gcagcacagt ggacagtgag
     6481 gtggtataca accgccggga tgtcaccaaa cggccaagag cggccagttg ccaccaaaat
     6541 tgggttgccc gacaacgaac gtcggagggg ccggccttgt cgggcattgg agccgctgcg
     6601 gacatcgccg atcagcagcg ggtgacttcg ttgcaaaatc atgcggttag gctacagatc
     6661 acggattgat ctgcaacatg tataaccgga cgtgcgcgtc cgtctattca gtagttagac
     6721 gttgcccaca atgcccacta tggacaccac aaaacccaaa gctgagcttt atctcatcgt
     6781 ccttctttgc gtgtcggcaa tcgttgttgg ccccttatca ctctgttcag tgctgcggcc
     6841 ggggtccgag tcgctcgcat cttggttcca gcgcagtggt gcaatcactt cgatttttgc
     6901 tgtgttcgcg cagtaccgtc tcagcgggtt cttggagtcc attagaggcg gcacatttgc
     6961 ggagtcttgg tcgctctacc acctgttcaa aacgcatcat cacgttctgt cttgggtcat
     7021 tgccgtcctc acaatttggg gcgcactcgt ctggggctac ggtgacctac ttctcaaata
     7081 cgtctaacat ttcgttcaag gcggacggct tcgacgccgc ttaactccag cgttgaagac
     7141 aggccttcct gaaagcagcc gtccgtgacc ggcagcaatg gaacgaaagc catcgttcgt
     7201 gactagtgaa ccaggtcggg atcagttgtt attgatccac tcctgtgcat cccggataaa
     7261 ccagggaaga ttcaactctt ccccctggcc ggactgtatg gtcaaacgct gacccgagga
     7321 accggtgctg ctgctcacac cctgcatcgt aagagatggc tggctctgat agtccaggtc
     7381 ggtttgcctg gacggattat ccgcgcaggc ggcgaccgac gcacacgcga ccgcgataaa
     7441 gactagccgc aatgttaagc gaaagctctt cataatgttc tggttcccgc tgatggcacc
     7501 agggcgaaac gtggcgttcc tgcaagtccg gcggctcttc gggagcgctc tgatggc
//
LOCUS       DASTNK010000029        22223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_15056, whole genome shotgun sequence.
ACCESSION   DASTNK010000029 DASTNK010000000
VERSION     DASTNK010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 22223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22223
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15056"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VFR20_02255"
     CDS             complement(<1..446)
                     /locus_tag="VFR20_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0229370.1"
                     /translation="MAFANPVTLSGEHARLELLSSKHHADLVEAVKDGELWKLWYTSV
                     PSPENVGAEIERRLGLFENGSMLPFAVIELATGQAVGMTTYMHIDAANRRVEIGSTWY
                     RRRVQRSGINTECKLMLLTHAFEELDCIAVEWRTHFLNHQSRAGIER"
     gene            complement(596..1648)
                     /locus_tag="VFR20_02260"
     CDS             complement(596..1648)
                     /locus_tag="VFR20_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEU0229371.1"
                     /translation="MGPHPSHDHATGQARGHGHDHDYRHGHDHGHDHGSHGGHAGHVH
                     SGHSHHHHPVPTDHNQAFVIAVSLNAVFVVVEFIYGLIADSSALVADAGHNLSDVLGL
                     LLAWGAAYLARKAPRGRYTYGLRSTSILAALANAMFLLVACGAIAWEVGQRFFQPPPV
                     AGFTVAVVAAVGIVVNGVSAWLFMRGSKADLNIRGAYLHLLADAVVSLGVVITGGVMM
                     LTHWYWLDPVVSLVIVVIIVIGTWGLLRESVQLALNAVPASIDVEAVEAFLRESPRVT
                     QVCDLHIWGMSTTENALTAHLVMPEGYPGDEFLDSLMRGLKERFSIQHSTLQVGLGTT
                     RYACSLEAPAPAGDAH"
     gene            complement(1648..1956)
                     /locus_tag="VFR20_02265"
     CDS             complement(1648..1956)
                     /locus_tag="VFR20_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU0229372.1"
                     /translation="MLAVQSDLGVAELADLFHLLGDATRLRIVIACLDAPASVGDIAE
                     RLVLSNSLVSHHLRLLRAARVVRFERRGRQVFYVAADRHISGMISGMLEHIAEPSEAN
                     "
     gene            complement(2062..3081)
                     /locus_tag="VFR20_02270"
     CDS             complement(2062..3081)
                     /locus_tag="VFR20_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229373.1"
                     /translation="MRDLAPIQVYAVPGTSVDAPRALPAPGTESPESEPAPEAPVLDL
                     YSFPNLGRQQTLPHRHTGLHWPAERGVASPGDTRRWFSTPDAGWTLGTDNWRYANTAG
                     LGLTLGSDTSQAPAWSAPVRLAGVGVHWDPDADASSNAWQYAMSVGAIDETPAAATQG
                     GLSYGPAASNSVLRYNVSPGISVNSQMQWTPGLRNVGLGGMYSLHDWGAWELGVSRAA
                     SAGQDGWGYQLGYSVDVLQDLKLSWTNQQRGAGYTDLSLYQAPLAGPSESNQWQATVP
                     LGRWGDLSGSYSQLDAAAGAVRQTLGVSQRFWYDPHVQVRLAANHDLVSGAYDLGVKF
                     SLPLN"
     gene            complement(3353..5140)
                     /locus_tag="VFR20_02275"
     CDS             complement(3353..5140)
                     /locus_tag="VFR20_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClcB-like voltage-gated chloride channel
                     protein"
                     /protein_id="HEU0229374.1"
                     /translation="MTRSPLPSLRKRLEGRLWLSDLPAMLGWAALVGVLGAFSTIGFH
                     EGIHLLQRAFTPSPGAIVAVTRTLPWYDRLLVPMAGGVIAGACLWAAARIQAGANSDY
                     MEAVAIGDGKLSIRQGLLRSLASLNTVASGGSIGREGAMVHLASLIASAIGRFACFGT
                     ARLRLLVACGAAAGVSSAYGAPIAGAMFVAEIILGTMAMQSLGPLLVAAAAANITMRL
                     TGVYHPNYEMSHVPLITAFEIVPFLALGVLTGIAAPLFLKFLALIKALFKKTALPLPA
                     RLGLGGLLLGCLLLFMPDVAGNGYSVVHSLLNTRWTWYAVLIVLMCKVLATALTVGSG
                     AVGGVFTPSIFVGAAFGTLFGQIMMWLWPATVSHPYLYTVVGMGAFLGAATSAPLMAI
                     LMIFEMTLSYQIVLPLMLACVVAYFISRAVAQVAMYNVTLLRERDVVLRRQLRNTALA
                     DLIRPADTVVVTTALVSEALRMFLDYPVKYLYVVDENNVYQGVIAQQDLTRLLLSDTD
                     AQHRCAGDVLRLDFVKTLHPDMALDEAQTYFVNFQGERLPVVSREDPHRLLGVVYKSS
                     LLERYCALKKSLDASGEVMLDVEAPRHSG"
     gene            5305..5904
                     /locus_tag="VFR20_02280"
     CDS             5305..5904
                     /locus_tag="VFR20_02280"
                     /inference="COORDINATES: protein motif:HMM:NF020348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HEU0229375.1"
                     /translation="MAEILGPQKLGPLTPLVGYWEGNVGVDLSYHNKDDETTQTGYFE
                     KAWFKPIPIVENGQQTMEGLNYEMTAWRHGEEAMDPFHDEVGYLLWDKKNGQVMRCFA
                     VPRGLAILAGCTAKAHDKTLKFVAEPGSASYGLSQNKYLLERAKATAFESTFTFNEDG
                     TFRYTSSLTLKLAATGSEMRHTDHNTLHRVARIHPSDEH"
     gene            6111..6812
                     /locus_tag="VFR20_02285"
     CDS             6111..6812
                     /locus_tag="VFR20_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0229376.1"
                     /translation="MDELLAKILVVDDDPALRQLLADYLNRHSYDTLLAPDASDLAAR
                     IQRYSPDLIVLDRMLPDGDGAQACRQLRQQGEDIPVILLTARDETVDRVIGLEAGADD
                     YLGKPFDPRELLARVEAVLRRKKGASALNKDKPVSFGPFVFDPATRQLYKDRVLIKLT
                     GGDINLLEALVSNPGKALSRERLLALARDDESGERNDRAIDIAVLRLRRSIEDDPRQP
                     RWIQTVWGVGYRFSP"
     gene            6816..8222
                     /locus_tag="VFR20_02290"
     CDS             6816..8222
                     /locus_tag="VFR20_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0229377.1"
                     /translation="MPRLPLPRSLRLRMVLLTLGTILLVQAATVATISYYRHQYSQNA
                     AVKFTATTIRILRASLAKVPPDERADFVRVSSQNEWHLWTQILPADTYIEGQYPKHHH
                     HKHAGGPVDTNHHLHRFVRELNAQLNDGTRVALSRGADPQLYISLKPDRENDVEDNVL
                     RREWLVIPVHRITAPVPTPVIIIWLAGMALFLLLAALFSWHITRPLTRLAKAADQFAA
                     GNPQRVVPSGPTETRALGERFNAMLDTLAESSAVRRTLLAGLPHDLKGPLSRMWLRIE
                     MANEAPFQDGMRKDIQEMQRMIDQFIGFVRGTDPGTYRFKPLNLNVWLDEQIGAWEST
                     GDTVRQIRHDNPPMTISADRSALTRLFDNLITNALNHGKPPVEVDLRAEFGLAVVTVR
                     DHGPGIPEARRAEALRPFARLDDARTRTGSVGLGLALADAITRAHGGTLELSDANGGG
                     LEITLRFPLAKPSGEPST"
     gene            complement(8311..9360)
                     /locus_tag="VFR20_02295"
     CDS             complement(8311..9360)
                     /locus_tag="VFR20_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEU0229378.1"
                     /translation="MISYLAALLGFSAQDPSFWMPLTLMGLLFAIIVAGTVLGGFDVG
                     VGCLVFFAPPGLRGRMLSLLGPWRDANEFWLFLGLGLFVTAFPGAWGAIMGRLYLPLC
                     LLALGVLLRSVSFELRLRSPRPMQAGWIRGFAVGSLLVAFAHGLLLGELVVNYQTRSG
                     YVWFSAFIGVCAFAAYCLLGACWLIMREAGELRVRAVVWGRRAVRWTAAGAVGVSVVL
                     VFANAGIFLKWTGPGMWRVMAGIWGAVFLAFVALEMRLQRMVNRSYRATSLPFVLALL
                     IFLIVLGGLWYSFFPFLVLDDMTIWDAAASVSALRLIVSAAVVALPVAVIFNVWVYWR
                     MFGLSRPPAPPPFKP"
     gene            complement(9357..10700)
                     /locus_tag="VFR20_02300"
     CDS             complement(9357..10700)
                     /locus_tag="VFR20_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEU0229379.1"
                     /translation="MMSNTALWLSAVQFFLSYGFMALFIALELGLAWALFYFKVRALW
                     SGNQAWTDAYRFWVRIFALAFFLGLAGSMPVLIQFGSLWPTLMVKIGEVAGPLLAAAI
                     LTTFIFKSCFLGAMLFGQRYVSDRVHALIVFMVAAGCSLALFWVLALQTWMQTPTGAS
                     LIEGRYYTGNWVHIIFNPSLPWYGGQVAVLAVLTAAFMMLGVTAGQTFRRPLVESERL
                     VFKNALIVAAVCVVLQGWVGAGTQRALAAYQPARMAATDAYWHSGAPPDLVLFAWPDE
                     ASASNHAAWVWHGAASPWLARDAKGRLQGLDRFAGMAPPVALTYWLFRLMLLIGLAMV
                     LVSWVTWWRVRKRQYDPTGLPLGWRRLLAAMTFSGWVACAVGLGYAAFGMYPYAVNDT
                     VTLREVSGDAGIWMLLAGTVAYLAFYGVLLWGFLGLLRHVARYGVVPVARRRGRA"
     gene            11022..12782
                     /locus_tag="VFR20_02305"
     CDS             11022..12782
                     /locus_tag="VFR20_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEU0229380.1"
                     /translation="MTSAPNPPASNFLRTIIENDLGAGRYADKRWAGRPGPAAVQEQG
                     LPDTAAIRTRFPPEPNGYLHIGHAKSICLNFSLAREFNGACHLRFDDTNPEKEEAEYV
                     ESIMDTVHWLGFDWNRDGESNCYFASDYFDYLYGFAEALVTAGHAYVDEQNPDQMRAT
                     RGTLTEPGTDSPWRDRPADESLRLLREMRDGRHPDGSMALRARIDMASPNMNLRDPVL
                     YRIRHAAHHRTGTQWTIYPMYAWAHPVEDALEGITHSICTLEFEDQRPFYDWILGKLA
                     DLGQLRRPLPRQYEFARLNLSYVVTSKRKLLQLVREGHVRGWDDPRMPTLVGLRRRGY
                     TPSAVRLFCERLGVSKSDSRIDYGLLEQALRDDLDPTAERRVAVLDPLKLVLTNFPEG
                     TNEPCQAPRNPHAPEQGTREFPLTRELWIERDDFREEPPKKYFRLYPGNTVRLKYGYI
                     VRCTGFDKDDNGVVTTVYAEYLPDTKSGSQSADAPPVKVKGAITWISAAHAVSAEVRL
                     YDRLFADPHPDAGDKNFLDALNPDSFHSVRGWIEPGTDARPGTILQFERLGYFVADQV
                     DSSADTPVFNRAVTLRDSWS"
     gene            12787..13569
                     /gene="minC"
                     /locus_tag="VFR20_02310"
     CDS             12787..13569
                     /gene="minC"
                     /locus_tag="VFR20_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEU0229381.1"
                     /translation="MAKTSSAPDRSLALDFKSATLYAVRVVLHSHDLAELLPALDQRM
                     RDAGAFFENEPVVIDATKIDEPLDWQALAEALRGHRLFPIGVMAQDDNLASAQALGLT
                     AVDLTSSAARVASKPAPEKETPAIAPPQNSGKAAAQKPAASAASAVAPAAMVINHPLR
                     SGQRIYARQTDLVVIGAVNQGAEVIADGNIHIYGPLRGKAMAGARGDTSARIFTTQLD
                     PELLAIAGVYRVIETRLEQPAHSHAMLVQLEGDALRIVPLDT"
     gene            13734..14549
                     /gene="minD"
                     /locus_tag="VFR20_02315"
     CDS             13734..14549
                     /gene="minD"
                     /locus_tag="VFR20_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEU0229382.1"
                     /translation="MARIVVVTSGKGGVGKTTTSASFSTGLAMRGHKTAVIDFDVGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIRDKQLENLFILPASQTRDKDALTQEGV
                     EKTLNGLKDMGFDYIVCDSPAGIETGALMASYFADDALVVTNPEVSSVRDSDRILGIL
                     SAKSKRALDGAEPIKEFLLLTRYSPKRVADGEMLSLKDIEDILRIKLIGVVPESESVL
                     QASNQGIPAIHLRESEVSLAYQDVVARYLGEEKALRFVDYEKPGLFKRLFGGK"
     gene            14551..14832
                     /gene="minE"
                     /locus_tag="VFR20_02320"
     CDS             14551..14832
                     /gene="minE"
                     /locus_tag="VFR20_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEU0229383.1"
                     /translation="MSFLSFLLGEKKTSASVAKDRLQLILINERGHGAATPDFLPRLQ
                     RELVEVISRYVKINPDDIKVNFDRQNSLEVLEVKIEMPPPEAARAADPA"
     gene            15836..16228
                     /locus_tag="VFR20_02325"
     CDS             15836..16228
                     /locus_tag="VFR20_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4168 domain-containing protein"
                     /protein_id="HEU0229384.1"
                     /translation="MKPWITTACLTALLALAGAAAPAMAQQTQAPAAQPPSPAAQKGP
                     AVNYSDAQLRKFVGASKKVAVVVQEYNPKLQSSPDEASREKIAQEANKKMVDAVQKEG
                     MSVNEFNSMSRAIQQDPTLMKRAQDIAK"
     gene            complement(16422..16970)
                     /locus_tag="VFR20_02330"
     CDS             complement(16422..16970)
                     /locus_tag="VFR20_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEU0229385.1"
                     /translation="MAGLRARDGVLLVVDLQTGLLPAIDGRAGLARRCEALARAAHLL
                     DVPVLATEHCPDKIGPTLPAVAGLVDRVVPKTHFDATREVGFISALPMGRPKVLVTGT
                     EAHVCVWQTALGLKEAGLDPVLVADCVGSRRPSDRATALRRAAFHGIEVVTSEMVMFE
                     WLESAGHPRFREVLALIKSTDD"
     gene            complement(17111..18784)
                     /gene="ettA"
                     /locus_tag="VFR20_02335"
     CDS             complement(17111..18784)
                     /gene="ettA"
                     /locus_tag="VFR20_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEU0229386.1"
                     /translation="MAQYVYTMNRVGKIVPPKRQILRDISLSFFPGAKIGVLGLNGAG
                     KSTLLKIMAGVDRDIEGEAVPMAGLKIGYLSQEPELDPGHTVRQSVEEGLGDVFSARQ
                     RLEEVYAAYAEPDADFDKLAAEQAELEAVIAAAASSGADDIEHQMEIAADALRLPAWD
                     AVVGNLSGGEKRRVALCRLLLSKPDMLLLDEPTNHLDAESVEWLEQFLRKFPGTVVGV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKENRLEQEEASESARQRTIKKEL
                     EWVRQNPKGRHAKAKARLARFEELSSHEYQKRNETQEIFIPVAERLGTDVIELNNVSK
                     AYGDRLLLDNVSFRIPAGAIVGVIGANGAGKSTLFRMIVGTEKPDAGEIRIGQTVRMG
                     YVDQSRHSLPDDKTVFEAVSDGADLLTVGRFEMASRAYLGRFNFKGADQNKRVGNLSG
                     GERGRLHLAKTLLSGGNVLLLDEPSNDLDVETLRALEDALLEFAGCVLVTSHDRWFLD
                     RIATHILAFEGDSRVVFFDGNYQEYEQDKKQRLGEEGAKPRRLRYKSLK"
     gene            18969..19445
                     /locus_tag="VFR20_02340"
     CDS             18969..19445
                     /locus_tag="VFR20_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229387.1"
                     /translation="MTASPSSAAPLTFTPHELELLARTADALSAYMGKPVLAEVVDAE
                     EAGFEWVIFAVPLESEATCDATVQIGGPNARLLGQKGGLTLHSDDVFECEFLWAIQLT
                     DVRGIRFIKIDGHGNEVAWTETLNEILPFVLNDTPVPEDDDNDEDDDDSPEAPTRQ"
     gene            complement(19489..20349)
                     /locus_tag="VFR20_02345"
     CDS             complement(19489..20349)
                     /locus_tag="VFR20_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU0229388.1"
                     /translation="MWRLLRFAVGAALAVQATAGWARDEAQLARGKYLMNGVVACANC
                     HIARGDKGQPLFDQGMSGGMLFEDSAFKAYAANITPDRETGIGAWTDAQIAKAVREGI
                     RPDGSVIGPPMPISFYRNLSDTDLAAIIAYLRAQPAVRHVVPKSVYHMPLPRRYGPPV
                     AHVSAPPASDVLKYGRYLANIGHCMDCHTPRDSQGRLVTASWGAGGQVIKWPGGKVVT
                     PNLTPDKSGLGTWTDAQIEHAIRDGVDNHGRHLAPLMAFDWYRNIDGADMHALIAYLR
                     ALKPLPFGGK"
     gene            complement(20565..21491)
                     /locus_tag="VFR20_02350"
     CDS             complement(20565..21491)
                     /locus_tag="VFR20_02350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEU0229389.1"
                     /translation="MNVWQYQFTVLTPSYNRAHTLGRVYASLCDQTFQDFEWLVVDDG
                     STDDTRQRVEAWQAEARFPIRYVWQANQHKKTAFNHGVREARGELIVALDSDDSLDIN
                     ALDAMNRAWRDIDPDARARYVAVTGLCARPDGHIVGDMFPRDVMDASSLDMTFRHHVG
                     GEKFGCLRTAVLRDFPFPEDIPGFVPESLVWRAIARAGYLTRFVNQVFRVYHDSADSL
                     SRQGRDGARHALGLYLLARDTVVECLPWFASRPTEFFKAALRCTRFGLHVARNGQAIP
                     ARYRVTGWKSQALVGLMWPAGVLLYVLDLLRA"
     gene            complement(21702..21830)
                     /locus_tag="VFR20_02355"
     CDS             complement(21702..21830)
                     /locus_tag="VFR20_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014111951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229390.1"
                     /translation="MAEIKRTRRNTLERRCLGKSFKRLFVKAPKGIFKMLEKVKRA"
BASE COUNT         4616 a         7411 c         6468 g         3728 t
ORIGIN      
        1 cgctctatgc cggcacggct ttgatggttg aggaaatggg tgcgccactc caccgcgata
       61 cagtccagtt cctcgaacgc atgcgtgagc agcatgagct tgcattcggt gttgatcccg
      121 ctgcgctgaa cgcgccgcct ataccatgtg gatccgattt cgacacgccg gttggcggcg
      181 tcgatatgca tgtaggtcgt catgccgacc gcctggccgg tggccagttc gatgaccgcg
      241 aacggcaaca tggagccatt ttcaaacaac cccagacgac gttcgatttc ggcccccaca
      301 ttctcgggcg atggcaccga tgtgtaccac agcttccaca gctcgccatc cttgacggcc
      361 tcgacgagat ccgcatgatg ctttgatgac agaagttcaa gccgggcgtg ttcgccggac
      421 agcgtgacgg ggttcgcgaa tgccatgggt acctgcctgg tgagttcggg gaaaattcac
      481 atgataactg cgtattccgt tgatcgtgga cggcgattcc ggcatcgtga ccaacgcgcc
      541 gcgatcgtga ccgatgatcg gtgacaacga ggtcaacaga ccgcgcgcag ccggatcagt
      601 gcgcgtctcc cgccggcgcg ggggcttcca gggaacaggc atagcgggtc gtccccagcc
      661 ccacctgcaa cgtgctgtgc tgtatggaaa accgctcttt caacccccgc atgagactgt
      721 ccaggaactc atcacccgga tagccctccg gcatgaccag atgcgcggtc aacgcgtttt
      781 cggtggtgct catcccccag atatgcagat cgcaaacctg cgtcacacgc gggctttcgc
      841 gcaggaacgc ctcgaccgcc tcgacgtcga tcgacgcggg gacggcattc agcgccaact
      901 gcacggattc gcgcagcagc ccccaggtgc cgatgacgat gatcaccacg atgacaaggc
      961 tgaccaccgg gtccagccag taccaatggg tcagcatcat gacgccgccc gtgatcacga
     1021 cacccaggga aacgaccgcg tccgccaaca aatgcagata cgcgccccga atatttaagt
     1081 ccgccttgct gccgcgcatg aagagccagg ccgatacccc attgaccacg atacccaccg
     1141 cggcgaccac ggccaccgtg aaccccgcca ccggcggcgg ttgaaagaag cgctgtccca
     1201 cttcccaggc gatggccccg cacgccacca gcaggaacat ggcattggcc agcgcggcca
     1261 ggatggaggt gctgcgcaag ccgtatgtgt agcgaccgcg aggcgccttg cgtgccaggt
     1321 aggccgcgcc ccaggccagc agcaggccca gcacgtcgga cagattatgg cccgcgtccg
     1381 ccaccagagc ggaggagtcg gcgatcaggc catagatgaa ttcgaccacc acgaacaccg
     1441 cattgagcga caccgcgatg acgaaggcct gattgtgatc cgtgggcacc gggtggtgat
     1501 gatgggaatg cccactgtgc acgtgaccgg catggccgcc gtgggagcca tggtcatgtc
     1561 catggtcgtg accgtgccgg taatcatgat cgtgaccgtg accacgggcc tggccggtcg
     1621 cgtgatcgtg cgaaggatga ggtcccatca gttggcctcc gagggttcgg cgatatgctc
     1681 cagcatgccg ctgatcatgc cggaaatgtg acggtcggcg gccacgtaga acacctgcct
     1741 gccacgtcgt tcgaaccgga ccacccgcgc cgcgcgcagc aggcgcaaat ggtggctgac
     1801 cagcgaattg ctcaggacca gccgctcagc gatgtcgccg accgacgctg gcgcgtccag
     1861 gcacgcaatg acgatgcgca accgcgtggc atcgcccagc agatggaaga gatcggccag
     1921 ttcggcgacc ccgaggtcgg attggacagc gagcatggaa acatatgatt gaatacgtgt
     1981 tcatatgtta ggtatgagtg cggcacaatg caagacattt atctctgatc tccaaggact
     2041 cttaaggatt ccaaaggatc cttaattcaa aggcagcgag aatttcaccc ccaggtcgta
     2101 agccccgctg acaaggtcgt gattggccgc gagacgcacc tgcacatggg ggtcgtacca
     2161 gaatcgctgt gacacgccca gggtctggcg cacggcaccg gcagcagcat cgagttgcga
     2221 gtacgaaccg ctgaggtcac cccatcggcc cagcggcacg gtggcctgcc actggttgct
     2281 ctcgctcggg ccggccagcg gcgcctggta gagcgacaag tccgtgtagc ccgcgcctcg
     2341 ttgctggttc gtccacgaca gtttcagatc ctggagcacg tcgacgctat agccaagctg
     2401 gtagccccag ccgtcttgcc cggcactcgc cgcccgcgac acccccagct cccaggctcc
     2461 ccaatcgtgc aacgaataca tgccacccaa gccgacattg cgcaagcctg gcgtccactg
     2521 catctgggaa ttcacggaga tgccgggcga aacgttgtag cgcagcacag aattggacgc
     2581 ggcgggcccg tagctcagtc cgccttgggt ggccgccgcg ggggtttcat cgatggcgcc
     2641 gacggacatg gcgtattgcc aggcattgct ggacgcgtcg gcgtcgggat cccaatggac
     2701 accgacgccg gccagacgaa ccggcgcgct ccaggcgggg gcctgtgacg tgtcgctgcc
     2761 cagcgtgagg ccaagtcccg ccgtattggc ataacgccag ttgtcggtgc caagcgtcca
     2821 tcccgcatca ggagtggaaa accatcgccg tgtgtcaccc ggcgacgcca cacctcgctc
     2881 ggccggccaa tgcaagccgg tgtgccgatg cggaagcgtc tgttgccgcc ccaggttcgg
     2941 aaaagaataa agatccagca ccggcgcctc gggtgccggc tccgattcgg gactctccgt
     3001 tccgggcgcg ggcagggctc ttggagcgtc gaccgacgtg cccggcaccg catagacctg
     3061 tatcggggcc agatcccgca aggtcggtcc tgtggaatcc gcccaggcac accccgccac
     3121 acctaccaaa ccacagagcg ccagcccccc gccaacccat ctccacacta ctttcatacc
     3181 tcgcttccca acaaatcgaa ccgcgcattc atcacggatc gaccaatcat ttccgaccac
     3241 ccacacaact tttcccggta catccgttcc aggggatccg ctgaaaaagc cacagtgcct
     3301 gccttccgct cgacttgttc agcggatcac aagcggacca gtccgcacct cgtcatccgg
     3361 aatgacgtgg cgcctcgacg tcgagcatga cctcaccgct ggcatcgagc gatttcttca
     3421 gggcgcagta ccgctccagc aacgacgact tataaaccac gcccagcagc cggtgggggt
     3481 cctcgcggga caccacaggc aggcgctcac cctggaaatt gacgaaatag gtttgcgcct
     3541 catccagcgc catgtcggga tgcagcgtct tgacgaaatc cagtctgagc acgtcgcccg
     3601 cgcaccggtg ctgcgcatcc gtgtcgctca acagcagacg ggtgagatcc tgctgggcga
     3661 tcacgccctg gtacacattg ttttcatcga ccacatacaa gtacttgacc gggtagtcca
     3721 ggaacatccg cagcgcctcg ctcaccagcg ccgtcgtaac cacgaccgtg tccgccggac
     3781 ggatcaagtc cgccaacgct gtattgcgta attgccgacg caacacgacg tcgcgttcgc
     3841 gcagcagcgt cacgttgtac atggccacct gggcgacggc acgggaaatg aagtacgcca
     3901 ccacgcaggc cagcatcagg ggcagcacga tctgatagct gagcgtcatt tcgaagatca
     3961 tcaggatggc catcagcggc gcgctggtcg ccgcgcccag gaaggccccc atgccgacga
     4021 cggtgtacag atacgggtgc gacacagtgg ccggccacag ccacatcatg atctgcccga
     4081 acaaggtgcc gaaggccgcg cccacgaaaa tggatggggt gaacaccccg cccactgccc
     4141 ctgaccccac ggtgagcgcc gtggccagaa ccttgcacat gagaacaatc agaacggcgt
     4201 accacgtcca gcgcgtgttg agcagcgagt gcacgacgct gtagccattg cccgccacat
     4261 caggcatgaa cagcagcagg caacccagca gcagcccgcc caggcccaac cgggccggta
     4321 gcggcagcgc ggtttttttg aacagcgcct tgatcagggc caggaatttc aggaacaacg
     4381 gcgcagcgat tccggtcaac acgcccagcg ccagaaaagg cacgatctcg aaggcagtga
     4441 tcaagggaac atgcgacatc tcgtagttgg ggtgataaac ccccgtcagg cgcatggtga
     4501 tgttagccgc ggcagccgcc accagcagtg gccccaggct ttgcatggcc atggtgccca
     4561 gaatgatctc agccacgaac atggcgcccg cgatcggagc gccataggcc gaggaaacac
     4621 ccgccgcggc cccgcaggcg accagcagac gcagcctggc cgtgccgaaa cacgcgaacc
     4681 ggccgatagc cgacgcgatc agcgacgcca gatgcaccat ggccccttcc cgcccgatgg
     4741 atccacccga cgccaccgtg ttgagcgacg ccagcgaccg gagcaaccct tggcggatgg
     4801 acaacttgcc atcaccgatg gcgaccgctt ccatgtagtc ggaattggcc cccgcctgta
     4861 tgcgggccgc cgcccacaga cacgcgccgg caatgacccc gccggccatg ggcacgagca
     4921 gtcggtcata ccaaggcaat gtgcgcgtaa ccgcgacgat ggcgcccggg ctgggcgtga
     4981 aagcacgctg cagaagatga atgccttcgt gaaaaccgat ggtcgaaaac gcccccagca
     5041 cccccaccaa tgcggcccat cccagcatgg cgggcaggtc ggacagccac agacggcctt
     5101 ccaggcgttt tcgcagcgac ggtaaggggg aacgggtcat gcggcgggca acttgttcag
     5161 aggattccaa ggcataacga tatacgatta tccgatgcca tgattgtttt tgactaacga
     5221 ttcgatgaac agaatcaatt gacgattcgg tcgaacaatg actattgtgg gattccctca
     5281 aaactttatt agtgaggttt caccatggct gaaatacttg ggccccaaaa actgggacca
     5341 ttgacacctc tggttggcta ctgggaaggc aacgtcggcg tcgatctctc ctatcacaac
     5401 aaagacgatg aaacgaccca gaccggctac ttcgagaaag cctggttcaa acccattccc
     5461 atcgttgaga acggtcagca gaccatggaa ggcctgaatt acgaaatgac cgcctggcgt
     5521 catggcgaag aagccatgga ccccttccat gacgaagtgg gttacctgct ttgggacaag
     5581 aaaaacgggc aggtcatgcg ctgcttcgcc gtgccgcgcg ggttggctat cctggctggg
     5641 tgcaccgcca aggcgcacga caaaaccctg aagttcgtgg ccgagcccgg gtcggcgtcg
     5701 tacggcttga gccagaacaa atatctgctc gaaagagcga aggccaccgc gtttgaaagc
     5761 acattcacgt tcaatgagga tggcacattc cgctacacct ccagcttgac cctcaagctc
     5821 gccgccacgg gcagtgaaat gcgccatacc gatcacaaca ccctgcatcg ggtcgcgcgc
     5881 atccacccca gcgacgaaca ctgatccaat ccgcaccacg ggccgcagca ccacacaccg
     5941 tgcgtgcccc ggcccccccg atgcgccaac ccacgaacgt ctgtccgggg gcggggttca
     6001 atcatgtcaa cacatgtggc tgccaataag acagcacaca gaggtaagta ggtatttcag
     6061 ttcatttccc ctgcttgcca gccagcgtcc gagccggcac acttcttggc atggatgaac
     6121 tgctcgcaaa aatactggtg gttgacgatg acccggcctt gcgccagtta ttggcagact
     6181 acctgaaccg ccacagctac gacacgttgc tcgcccctga tgccagcgac ttggccgcgc
     6241 gtatccagcg atacagcccc gacctcatcg tgctcgaccg catgctgccc gatggcgacg
     6301 gtgcacaggc ctgccgccaa ttgcgccaac agggcgaaga cattcccgtc atcctgctca
     6361 ccgcccgcga cgaaaccgtc gatcgtgtca ttggcctgga agccggtgcc gacgactacc
     6421 tgggcaagcc gttcgatccg cgtgaactgc tggcgcgcgt cgaggcggtg ctgcgccgca
     6481 agaaaggcgc gtcggccctg aacaaagaca aacccgtgtc gttcggcccg ttcgtgttcg
     6541 atccagccac ccgccaactg tacaaggaca gggtgctcat caagctcacg ggtggcgaca
     6601 tcaacctgct cgaagcgctc gtcagcaacc ccggcaaggc cttgtcccgc gaacggctgc
     6661 tggccctggc gcgcgacgac gaatccggcg agcgcaacga ccgcgccatc gacatcgccg
     6721 tgttgcgcct gcgccgatcc atcgaagacg acccacggca accccgctgg atccagacgg
     6781 tctggggcgt cggctaccgc ttttccccct gatccatgcc gcgccttcct ctgccgcgct
     6841 cgctgcgcct gcgcatggtg ctgctgacac tgggcaccat cctgctcgtg caggccgcca
     6901 ccgtcgccac catttcctac taccgccatc aatactcgca gaatgccgcc gtcaaattca
     6961 cggccaccac catccgcatt ctgcgcgcgt cgctggccaa ggtacccccc gacgagcgcg
     7021 ccgatttcgt gcgcgtgtcg tcgcagaacg aatggcatct ctggacgcag atactgcctg
     7081 ccgacacgta catcgagggc cagtatccca agcatcacca tcacaagcac gccggcgggc
     7141 ctgtcgatac caaccatcac ctgcaccgtt tcgtgcgcga gctcaacgcc cagctcaatg
     7201 acggaacccg tgtcgcgctg tcgcgcggcg ccgatccgca actctatatt tcattgaaac
     7261 ccgaccgcga aaacgacgtc gaagacaatg tcctgcgacg cgaatggctg gtcattcccg
     7321 tacaccgcat caccgccccc gtgcccacgc cggtcatcat catttggctg gccggcatgg
     7381 cgttgttcct gctgctcgcg gccctgtttt catggcacat cacccgcccc ctgacgcggc
     7441 tcgccaaggc cgccgaccag ttcgccgcgg gcaaccctca gcgcgtggtg ccgtctggtc
     7501 ccaccgagac ccgcgccctg ggcgaacggt tcaacgccat gctcgataca ctggcggaat
     7561 ccagcgcggt gcgccgcacg ctgctggccg gtttgccaca cgatctgaaa gggccgttgt
     7621 cacgcatgtg gctgcgcatc gaaatggcca acgaggcgcc atttcaggac ggcatgcgca
     7681 aggacatcca ggaaatgcag cgcatgatcg atcagttcat cggtttcgtg cgtggcaccg
     7741 acccaggcac ttaccgcttc aaaccgctga acctcaatgt ctggctggac gaacagatcg
     7801 gcgcctggga aagcaccggt gacactgtgc gccagatccg gcacgacaac ccgcccatga
     7861 ccatcagcgc cgatcgctcc gccctgacgc ggctgttcga caacctgatc accaatgccc
     7921 tgaaccatgg caaaccgccc gtcgaggtgg atctgcgggc ggaattcggt ctcgcggtgg
     7981 tcaccgtccg cgatcacggc cctggcatcc ccgaggcgcg ccgcgccgag gccttgcgcc
     8041 ccttcgcgcg gctggacgat gcccgcaccc gcacaggctc ggtgggcctg gggctggctt
     8101 tggcggacgc catcacgcgg gcgcacggcg gcaccctcga actgagtgat gccaatggtg
     8161 gcggactgga aatcacgctc aggtttccac tggcgaagcc ttccggcgaa ccgtcaacct
     8221 gatgcccgct cagagggcat tccattccaa tatcgcgaag cgtagatgaa gaaccaaagc
     8281 ggggtggttg aatcagccaa ccccgtgccc ctacggtttg aacggcggcg gcgccggcgg
     8341 gcgcgatagg ccgaacatgc gccagtacac ccatacattg aagatcaccg ccaccggcag
     8401 cgccaccacg gccgccgaca cgatcaggcg caacgcggac accgaggccg cggcatccca
     8461 gatggtcatg tcatcgagca ccagaaatgg aaaaaagctg taccacagtc cgccaagcac
     8521 aatcaggaaa atcagcagcg ccagcacgaa cggcagcgag gtggcgcggt aactgcggtt
     8581 gaccatgcgc tgcaggcgca tctcgagcgc cacgaaagcc aggaacacgg ccccccagat
     8641 gccggccatg acgcgccaca tgccgggtcc cgtccatttc aggaaaatac ccgcgttggc
     8701 gaacaccagc acgaccgaca cccccaccgc gccggccgcc gtccaacgca cagcccgcct
     8761 tccccacacc accgcccgca cccgcagctc accggcctcg cgcatgatga gccaacacgc
     8821 ccccaacaga cagtacgcgg caaaggcgca cacgccaatg aacgcggaaa accatacata
     8881 ccccgaccgg gtctggtagt tcaccaccag ttcgccgagc agcaggccat gcgcgaacgc
     8941 caccagcaac gacccgacgg cgaagccccg gatccagccc gcctgcatgg gccgtggcga
     9001 gcgcaatcgg agctcgaacg acaccgagcg caacagcacc cccaaggcca acaggcacaa
     9061 aggcaggtac aggcggccca tgatggcccc ccatgctccc gggaaggccg tcacgaacag
     9121 gcccagcccg agaaacagcc agaattcgtt cgcgtcacgc cagggaccca gtagcgaaag
     9181 catgcgccca cgcaggccgg gtggcgcgaa aaacaccaga cacccgaccc cgacatcgaa
     9241 ccctcccagg acggttcccg ccacgatgat ggcgaaaagc agccccatga gcgtcagcgg
     9301 catccagaac gacggatcct gggcactgaa cccaagcagc gcggcaagat aggaaatcat
     9361 gcccgccccc ttcgacgcgc caccggcacc acgccatagc gcgccacatg ccggagcaac
     9421 cccaggaacc cccacagcag caccccgtag aacgcgagat acgccactgt gccggccaac
     9481 agcatccaga tccccgcatc gccggaaacc tcacgcaggg taacggtatc gttgacagca
     9541 tagggataca tgccgaaggc tgcatacccc agccccacgg cgcacgccac ccagcctgaa
     9601 aacgtcatgg cggcaagcaa ccggcgccat ccaagaggca gcccggtcgg gtcatattgc
     9661 cgcttgcgca cgcgccacca agtgacccag ctgaccagca ccatcgcaag cccgatcagc
     9721 agcatcagcc ggaacaacca gtacgtcagc gccactggcg gcgccatgcc ggcgaatcga
     9781 tccagcccct gcagcctgcc cttggcatcg cgcgccagcc aggggctcgc cgcgccatgc
     9841 cacacccagg cggcatggtt gctcgccgac gcttcatcgg gccacgcgaa cagaaccaga
     9901 tcgggtggcg ccccgctgtg ccaataggca tcggtggcgg ccatccgagc cggctggtac
     9961 gcggccaggg cgcgttgcgt cccggcgccg acccaaccct gcaacaccac gcagaccgcc
    10021 gccacgatca atgcgttctt gaacaccagg cgttcgcttt cgaccagcgg gcgcctgaac
    10081 gtctgccccg ccgtaacgcc cagcatcatg aacgccgccg tgagcaccgc cagcaccgcc
    10141 acctggccgc cataccaggg aagcgaggga ttgaatatga tgtgcaccca gttgccggtg
    10201 taatagcggc cttcgatcag gctggcgccg gtgggggttt gcatccaggt ctgcagcgcc
    10261 aggacccaga acagggccag cgaacaaccc gccgcgacca tgaacacgat gagcgcatgc
    10321 acccgatcgg acacatagcg ctgaccgaag agcatggccc ccaggaaaca cgatttgaaa
    10381 atgaaggtgg tcaggatggc ggcggccagc aacgggccgg ccacctcgcc gatcttgacc
    10441 atcagggtcg gccacaggct gccgaactgg atcagcaccg gcatgcttcc agcgaggccc
    10501 aggaagaacg ccagtgcgaa aatgcggacc cagaaccgat aggcatcggt ccaggcctga
    10561 ttgcccgacc acaaggcacg caccttgaaa tagaacagcg cccaggcgag gcccagttcc
    10621 agagcgatga acagcgccat gaaaccgtag ctgaggaaga actggacggc ggacagccac
    10681 agggccgtgt tgctcatcat gtgcgccggt acggctcccc ttttaatgaa agacaggccc
    10741 tagtgtagtg tttgacgcta actggaacaa ataaaaccga tggattcctg gtctggacaa
    10801 ggcgcgaccc gcagagatgg tctttgctat cgcgcggatt tgcaacgtgg cagacagccg
    10861 caagccgcag atacatgcgg cgcggtgact tgttccgtgg atccctggat aaggcgaatt
    10921 gcgtcctcgt ggccgacgcc ctgcaagcgg gtcccacggc aagcggcccg gaacgtggca
    10981 aaatgctcac ttcgccagac actccacccg tcttcacctt catgacctcc gctccaaatc
    11041 cgcccgcctc caatttcctt cgcaccatca tcgaaaacga tctgggtgcg ggccgctacg
    11101 cagacaaacg ctgggcgggc cggcccgggc cggccgcagt ccaagagcaa ggcctgccgg
    11161 acaccgccgc catacgcacc cggtttccgc cggaacccaa tggctatctg catatcgggc
    11221 acgcgaaaag catctgcctg aacttcagcc tggcgcgcga attcaacggc gcctgtcacc
    11281 tgcgtttcga cgacaccaat ccggaaaaag aagaggccga gtacgtcgag tccatcatgg
    11341 acaccgtgca ctggctgggg ttcgactgga atcgggatgg tgaatccaac tgctatttcg
    11401 ccagtgatta cttcgattac ctgtacggat tcgccgaggc gctggtcacc gccggtcacg
    11461 cctacgtcga cgaacagaac cccgaccaga tgcgcgccac gcgcggcacg ctcaccgaac
    11521 ccggcaccga ctccccatgg cgcgaccgcc cggcggacga atcgctgcgc ctgctgcgcg
    11581 aaatgcgcga cggccgccac ccggacggca gcatggcgct gcgggcccgc atcgacatgg
    11641 cctcgcccaa catgaacctg cgcgatccgg tgctctaccg gatccgccac gcggcgcatc
    11701 atcgcacggg cacccaatgg accatctacc ccatgtacgc ctgggcccat ccggtcgaag
    11761 acgccctgga aggcatcacc cacagcatct gcacgctcga attcgaagac cagcgcccgt
    11821 tctacgactg gatcctcgga aaactcgccg atctgggcca actgcgccgc ccgctgccgc
    11881 gccaatacga attcgcccgg ctgaacctca gctacgtggt gaccagcaaa cgcaaactgc
    11941 tgcaactggt gcgcgaaggc catgtgcggg gctgggacga cccgcgcatg ccgacactgg
    12001 tggggctgcg ccgccgcggc tacaccccct cggccgtccg cctgttctgc gagcggctgg
    12061 gcgtctcgaa gtccgactct cgcatcgact acggcctgct cgagcaggcg ctgcgtgatg
    12121 atctcgatcc cactgcggaa cgccgggtcg ccgtgctgga tccgctcaag ctggtgctga
    12181 cgaacttccc ggaaggcacc aacgagcctt gccaggctcc tcgcaaccca cacgcccccg
    12241 aacagggcac gcgcgaattc ccactcacgc gcgaactatg gatcgagcgc gacgatttcc
    12301 gcgaagaacc gcccaagaag tacttccgtc tgtatccggg caacaccgtg cgcctcaaat
    12361 atggctatat cgtgcgttgc accggattcg acaaagacga caacggcgtc gtgaccacgg
    12421 tctacgccga atacctgccc gacaccaaga gcggcagcca gagcgccgac gctccgccgg
    12481 taaaggtcaa gggggcgatc acctggatca gcgcagcgca cgccgtctcc gccgaagtcc
    12541 gtctgtacga ccggctgttc gccgatccgc accccgacgc cggcgacaag aacttcctgg
    12601 acgcgctcaa tccggactcc ttccattccg tgcgcggctg gatcgagccg ggcaccgatg
    12661 cccggccggg cacgatcctg caattcgaac gcctgggcta tttcgttgcc gaccaggtcg
    12721 attcgtccgc cgacacaccg gtcttcaacc gcgcggtcac tctgcgcgat tcctggagct
    12781 gaccccgtgg ccaagacctc ctccgcgcca gatcgatcgc tggcgcttga tttcaagagc
    12841 gccacgctgt atgccgtgcg cgtcgtgctg cacagccatg atctcgccga actgctgccc
    12901 gcgctggacc agcgcatgcg cgacgccggg gcgttcttcg agaacgaacc cgtggtcatc
    12961 gatgccacca agatcgacga gcccctggac tggcaggcgc tggccgaggc cttgcgcggc
    13021 caccgcctgt tccccatcgg ggtgatggcg caagacgaca atctggcctc ggcgcaggcc
    13081 ctgggcctga ccgccgtgga tctgaccagt tcggcggcgc gcgtggccag caaacccgcg
    13141 ccggaaaagg aaacacccgc catcgcgcca ccacagaaca gcggaaaggc cgcggcgcag
    13201 aaaccggccg cgtcggcggc ctccgccgtg gcgcccgcgg ccatggtgat caaccatcct
    13261 ttgcgctccg gccaacgcat ctatgcccgc cagaccgatc tggtggtgat cggcgccgtc
    13321 aaccaagggg ccgaggtcat cgccgacggc aacatccata tttatgggcc gctgcgcggc
    13381 aaggccatgg ccggcgcccg gggcgacacc agcgcacgca ttttcacgac gcagctcgac
    13441 cccgaactgc tggccatcgc gggggtgtat cgggtgatcg aaacccgcct cgaacagccg
    13501 gcccactcgc acgccatgct ggtccaactc gaaggcgacg cgcttagaat cgttccgctg
    13561 gacacctgat ccaggctttc gcgcccgcca cctggcaagc aggcggcggg cgtatggcgg
    13621 tgattcaaca ttttgttgct aaccgattat caaataacga caatgcggga tcgtcaaagc
    13681 tgtttttgct ttacgattac gtaaatttcg aatatcagag ggattcaatc gacatggctc
    13741 gaatcgtagt ggtgacctcc gggaaaggcg gggtggggaa aaccaccacc agcgccagct
    13801 tttcaactgg attggccatg cgcgggcaca aaaccgccgt catagatttc gacgttggcc
    13861 tgcgcaacct tgacttgatc atgggctgcg aacggcgggt cgtctacgac ttcgtcaatg
    13921 tcatccaggg cgacgccacc ctgaaccagg ccctcatccg cgacaagcaa ctcgagaatc
    13981 tgttcattct gccggcctcg caaacgcgcg ataaagacgc gctgactcag gaaggcgtcg
    14041 aaaagaccct caacgggctc aaggacatgg gcttcgacta cattgtgtgc gattcccccg
    14101 ccggcattga aacaggcgcc ctcatggcct cgtatttcgc cgacgacgcc ttggtggtca
    14161 cgaatcccga agtctcgtcc gtgcgcgact ctgaccgtat cctgggcatt ctgtctgcca
    14221 aatccaagcg ggcgcttgat ggcgcagagc ccatcaagga gttcctgctg ctgacgcgct
    14281 acagccccaa gcgggtggcc gatggtgaaa tgctgtcact caaggacatc gaagacatcc
    14341 tgcgcatcaa actgattggc gtcgtgcctg aatcagaatc cgtgctacag gcgtccaacc
    14401 agggcatccc cgccatccat ctgcgtgaaa gcgaagtttc cctggcctac caggacgtgg
    14461 tggcgcgtta cctgggcgag gaaaaagctc tgcgctttgt cgattacgag aaaccggggc
    14521 tgttcaagcg ccttttcgga ggtaagtgac atgtccttcc tgtctttcct gctcggcgaa
    14581 aagaaaacgt cggccagcgt ggccaaggat cgcctgcaac tcatcctgat caatgaacgc
    14641 ggccatggcg ccgccacgcc tgatttcctg cccaggctgc aacgcgagct ggttgaggtc
    14701 atctcgcgct acgtcaagat caatcccgac gacatcaagg tcaacttcga taggcagaat
    14761 tccctggaag tgctggaagt caagatcgaa atgccgccac cagaggctgc gcgcgcggcc
    14821 gatccggcct gacaggaagt caaccgacac tgccttggcg cgtttgcgat ttactttggt
    14881 ttgcctaccc gcatgaagca aagcagttga agcattcatc gggggtaagc agctccgcgt
    14941 cgatggcccc catcggcgga ctatggcgct ggccgtattc ttgccatcgc gtacgcacct
    15001 ggcacgccca caagcgaccc gcttagtagc gcagggcgat cgccgaaggt cttttaccac
    15061 gctccagcgc ctccaggact caatcccgaa gatcttgcga atagatatgc atgaccatct
    15121 ccatcagttg tgatagagaa gagtccatat tatgtatatc tccaaatcaa aaatgctcta
    15181 ggccgcagcc gcgctgatcc agtgccaggc tcgacggaaa cagcgggcgc ggggaaaacg
    15241 cccggaggct ggttttagcc cggatcgtgg acggtcttgt atgcagttgt taaatctcgg
    15301 atctcgtacg tccgacgctc aacagaagct gttccctcta tatccgcctg atctggtcgc
    15361 tattcggaca atgatagtga aacccgcgtt tacatgtagg ggtttacccg taacgccggg
    15421 attggtacgg ttgacaacaa gccggttgta acagatcagc tgcctggcta ggccgcagtc
    15481 aagcgactgc ggcattcata ttccataagg ggaacatcat gaatacgatt cacagaacat
    15541 tgactcttgc cgccatggcc gcttcactga gtttgctggc cgcgtgctcc aaaggcggcg
    15601 acgattccgc cccgccgccg gcaggcggga cctcctcttc ctcaccagcc caggcgtctc
    15661 ccgccgcccc ctcttcgtca cccgcccctg ctccggcgcc tgcgatgccg tctccagccc
    15721 ctgcgtcccc cgctccgcag aaatgaggct aatcacactg cgggcaatct gacagaaaca
    15781 ccattcctgt ccggaatccc ccggccctgg cggtcgaggg cgaaaaggag tttgcatgaa
    15841 accatggatc actaccgcat gtttgaccgc gctgctcgca ttggctggcg cggctgcccc
    15901 cgcgatggcg cagcagacac aggctcccgc tgcgcaaccg ccatcgccgg ccgcgcagaa
    15961 ggggcccgcc gtcaattatt cagacgcaca gctcaggaaa ttcgtcggcg catccaagaa
    16021 ggtcgctgtc gtggtgcagg aatacaaccc caagctgcaa tcgtcgccag acgaagcctc
    16081 ccgcgaaaaa atcgcccagg aagctaacaa gaaaatggtc gatgccgttc agaaagaagg
    16141 gatgagcgtc aatgaattca acagcatgag ccgggcgatt cagcaagatc caacgctgat
    16201 gaagcgggcg caggacatag ccaaatagca acgagagtca gcgtggcgaa gagagttgcc
    16261 atcagcccga cacagcgtcg ggcgcgtcgg cccaacattg gcggcgcgcc ccggccgcgc
    16321 tggaactgcc ttcctcaggt cgacctggca tcgttatccc gccttcaaaa cacccgccgg
    16381 ccagagattt tttcgaaaag cccgccaatg acatatcggg gctaatcgtc cgttgacttg
    16441 atcaacgcca acacctcgcg aaaacgcgga tggccggccg actccagcca ttcgaacatg
    16501 accatttcgg acgtcacgac ttcgatgccg tgaaacgcgg cccgccgcag ggccgtggcg
    16561 cgatcggacg ggcgccgcga ccccacgcag tcggcgacca ggacgggatc caggcccgct
    16621 tctttaaggc ccaacgctgt ctgccacacg cacacatgag cttcggtgcc ggtcaccagc
    16681 actttgggcc ttcccatcgg cagagcggag atgaagccga cttcgcgcgt tgcgtcgaaa
    16741 tgggtcttgg gcacgacacg gtccaccaat ccggccaccg ccggcagcgt cgggccgatc
    16801 ttgtcggggc aatgctcggt ggcgaggacg ggaacatcga gcagatgcgc cgcccgcgcc
    16861 agcgcctcgc atcgacgcgc caatcccgcc cggccatcga tggcgggcag cagcccggtc
    16921 tgcaaatcga ccaccagcaa cacaccgtca cgcgcgcgta atccggccat gctgtgctcc
    16981 acaatgtgaa tgtcctggct catcaggcaa ggcacgagct gcggaaactg acgttgccat
    17041 cgcggggatt cgcaacgtca gatgaggaac gcgggcgccg aagagttgaa tcaacaagcc
    17101 aggcatccgc ctacttcaat gatttataac gcaggcgccg cggcttggcg ccctcctcgc
    17161 ccaggcgttg cttcttgtcc tgctcgtatt cctggtagtt gccatcgaaa aacaccacgc
    17221 gggagtcccc ctcgaacgcg aggatatgcg tggcgatgcg atccaggaac cagcggtcgt
    17281 ggctggtgac cagcacgcaa ccggcgaatt ccagcaaggc atcttccagc gcgcgcaggg
    17341 tttccacgtc caggtcgttc gacggttcgt cgagcagcaa gacgttgcct ccggacagca
    17401 gcgtcttggc caagtgcagc cgcccccgct ccccgcctga caagttgccc acacgcttgt
    17461 tctgatcggc gcctttgaag ttgaagcggc ccaggtaggc gcgcgaagcc atttcgaaac
    17521 ggccgaccgt cagcagatcc gcgccatcgg atacggcttc gaacactgtc ttgtcgtcgg
    17581 gcagcgaatg gcgggactgg tcgacatacc ccatgcgcac ggtctggccg atccggattt
    17641 cgccggcatc gggcttttcc gtgccgacga tcatgcggaa cagcgtcgac ttgcccgcgc
    17701 cgttggcgcc gatgaccccg acaatcgctc ctgccggaat gcggaagctg acgttgtcga
    17761 gcagcaggcg gtcaccatac gccttggaga cgttgttcaa ttcgataacg tcggtgccca
    17821 gccgctcggc gaccgggatg aagatttcct gggtttcgtt gcgcttctgg tactcatgcg
    17881 aagaaagttc ttcgaaacgc gcgagacgag ccttggcctt ggcgtggcga cccttgggat
    17941 tctggcgcac ccattccagt tccttcttga tggtgcgctg gcgggcggat tccgatgcct
    18001 cttcctgttc gaggcggttt tccttctgct cgagccagga actgtagttg cctttccagg
    18061 gaattccgtg cccgcggtcg agttcgagta tccattccgc cgcgttatcg aggaagtagc
    18121 ggtcgtgcgt aaccccgacc acggtaccgg ggaatttgcg caggaattgc tcgagccact
    18181 ccacgctttc cgcatccagg tggttggtgg gttcgtccag cagcagcatg tcgggcttgg
    18241 acaacagcaa gcggcacagc gcgacgcggc gcttctcgcc cccggacaga ttacccacca
    18301 ccgcgtccca ggccggcagg cgcaaagcat cggccgcgat ctccatttga tgctcaatgt
    18361 cgtccgcgcc gcttgacgcc gccgccgcga tcacggcctc gagctcggcc tgttctgccg
    18421 cgagcttgtc aaaatcggca tcgggttcgg cataggccgc gtagacctct tccagccgct
    18481 ggcgggccga gaacacgtcg cccagcccct cctccacgga ttgacgcacc gtgtggccag
    18541 ggtcgagttc gggctcctgc gacaggtagc cgatcttcag tccggccatg ggcacggctt
    18601 cgccttcgat gtcgcggtcg acaccagcca tgatcttcaa caaggtggac ttgcccgcgc
    18661 cgttcagccc aagcaccccg atttttgcgc cagggaaaaa agacaacgag atgtcgcgca
    18721 ggatctgccg cttgggcggt acgatcttgc cgacacggtt catggtgtag acgtattggg
    18781 ccatggttgc agactcggaa ttccggaatg gtcgtaaacc gatgattgta ggcgatgccg
    18841 taaaatggcc cagggatcct ctgaacaaat cgagcggaaa gcaggcgctg tggatcggga
    18901 tcggatgccc tcggaaaacc tccccagtgc cgccccgatt catcgtccaa cctagaccac
    18961 cgcccccaat gaccgcatca ccgtcctccg ccgcgcccct gaccttcacg ccgcacgagc
    19021 tcgaactgct ggcgcgcacc gccgacgccc tgagtgccta catgggcaaa cccgtactgg
    19081 cggaagtggt cgacgccgaa gaggctggtt tcgaatgggt gatattcgcc gttcccctgg
    19141 aaagcgaggc aacctgtgac gccaccgtgc agatcggcgg ccccaacgcg cgcttgctgg
    19201 gccagaaagg cgggctgacg ctgcacagcg atgacgtgtt cgaatgcgaa ttcctctggg
    19261 ccattcagtt gaccgacgtc aggggcatcc gcttcatcaa aatcgacgga cacggcaacg
    19321 aagtcgcctg gactgaaacc ctgaacgaga tattgccgtt cgtgctcaac gacaccccgg
    19381 tgcccgagga cgatgacaac gacgaggatg acgacgactc gcccgaggcg ccgacacggc
    19441 agtagcgctg gcttaggcag tagcgctggc ttaggcagta acgccggttt attttccgcc
    19501 gaacggtagc ggtttcaacg cgcgcaggta ggcgatcaac gcgtgcatgt cggcgccgtc
    19561 gatgttccgg taccagtcaa aagccatgag cggcgccagg tgcctgccat ggttgtcgac
    19621 gccatcgcgg atggcgtgtt cgatctgggc gtcggtccag gttcccaggc cggatttgtc
    19681 tggcgtgagg tttggcgtca ccaccttgcc tcccggccat ttgatcacct ggccacccgc
    19741 gccccaactg gcggtgacca gcctgccctg gctgtcgcgg ggtgtatggc agtccatgca
    19801 atggccgatg ttggccagat accggccata tttcaatacg tctgatgcgg gtggtgcgct
    19861 gacgtgcgcc acgggcggcc catagcggcg cggcaacggc atgtggtaga cggatttcgg
    19921 cacgacgtgc cgcaccgctg gctgggccct caggtaggca atgatcgcgg cgaggtcggt
    19981 gtcggacagg tttcgataaa aagaaatggg catgggcggc ccgatcacgc ttccgtcggg
    20041 gcggatgccc tcgcggacgg ccttcgcgat ctgggcgtcg gtccatgcgc cgattccggt
    20101 ttcacggtcg ggcgtgatgt tggccgcgta ggccttgaac gcgctgtcct cgaacagcat
    20161 tccgccggac atgccctgat cgaacaaggg ctgccccttg tcgccccggg caatgtggca
    20221 attggcacag gccaccacgc cgttcatcag gtatttgccg cgcgccagct gggcttcgtc
    20281 ccgcgcccag cccgctgtgg cttgaacagc cagcgcagcg ccgacggcga accgcaagag
    20341 tctccacatg gcgcccctcc tcaatcatga ctcatttgat gtgtcttctg ctcagcgacc
    20401 gttgtttcga ttccagttct tcttgtcgct acagcgtgcc agcatattac gctcggcatc
    20461 agcaactcac cataggccag ttggcccgcg catggggcct tgggtctatc tgcccgtccc
    20521 cttctgctcg tcagtgcggg gcgatcattt agtgtaaatc ggccctacgc ccgcagcaga
    20581 tccagcacat acagcagcac gccagccggc cacatcagcc ccaccagcgc ctgcgatttc
    20641 catcccgtga cgcggtatcg ggcagggatg gcctggccgt tccgggccac gtgcaagccg
    20701 aaacgcgtgc agcgcagcgc cgccttgaag aactctgtcg ggcggctggc gaaccagggc
    20761 aggcactcca ccacggtatc gcgcgccagc agatacaggc cgagcgcgtg gcgggcgcca
    20821 tcacgccctt ggcgcgacag ggagtcggcg ctgtcgtgat agacgcggaa tacctggttc
    20881 acgaaccgcg tcaggtagcc cgcgcgggcg atggctcgcc acaccaggct ttcgggcacg
    20941 aagcccggaa tgtcttccgg gaacggaaaa tcgcgcagca ccgcggtgcg caggcacccg
    21001 aatttctcgc cccccacatg atggcggaac gtcatgtcca atgacgacgc gtccatgacg
    21061 tcgcgcggaa acatgtcgcc cacgatatgg ccgtcgggtc tggcgcaaag gcccgtgacc
    21121 gccacgtagc gcgcacgcgc atcggggtca atgtcgcgcc aggcgcggtt catggcatcg
    21181 agcgcattga tgtccaggct gtcgtcactg tcgagcgcga cgatcagttc gccacgcgct
    21241 tcgcgcacgc catgattgaa agccgtcttc ttgtgctgat tggcctgcca cacgtagcgg
    21301 atggggaacc gggcctccgc ctgccaggct tccacgcgct gacgcgtgtc gtcggtcgac
    21361 ccgtcgtcca ccacgagcca ttcaaaatcc tggaacgtct ggtcgcacag tgacgcgtag
    21421 acgcgcccaa gcgtatgcgc ccgattgtag gagggggtga gaacggtgaa ctgatactgc
    21481 cacacattca tggaagcctg cgtctccggg aaggctgata ttggtttgcg cacgatcata
    21541 gggcaaaaaa aagccgccga accagatacg atcggcggat tttccgttgg tggcaaactg
    21601 aaacaaccgt taaccacttg gtcgatacaa gatcgacaca tacacaccaa gcgttccaga
    21661 gctacccgcc cccggaactc ctggcgcgtt tcaccctttc ttcaggcgcg ttttaccttt
    21721 tcaagcatct tgaaaatgcc tttcggcgct ttcacgaaaa gccgcttgaa cgatttaccc
    21781 aggcaacggc gctcgagcgt gttgcgacgt gtgcgtttga tttccgccat aatgttctcc
    21841 gaacgtgaac cgtcggccgg attcgcaccg ccgacgtgat tctttgacct cccggcaccc
    21901 atgccagtgg cgacggcgca cgcgagacct gaaagaacca gacaaaagct aaggacaccg
    21961 ccgattcgct caaacacaag caaaaagcgt tgcctaaggc aaaaaatatc agttaaccca
    22021 cgattctacc gtaaaaatgg acgaaaggct ggccacaacc cttgcagggc ggccagccga
    22081 gaacacagaa tgagctcaca gatacgcatc tttggcgcgc gccagaacaa cctgaagaac
    22141 ctggatgtct ccatcaacac cggcgaacta ctcgtcctga cgggcgtgtc ggggtcgggc
    22201 aagagttcgc tggccttcga cac
//
LOCUS       DASTNK010000030        13388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1499782, whole genome shotgun sequence.
ACCESSION   DASTNK010000030 DASTNK010000000
VERSION     DASTNK010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13388
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499782"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..140
                     /locus_tag="VFR20_02360"
     tRNA            65..140
                     /locus_tag="VFR20_02360"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:98..100,aa:Asn,seq:gtt)
     gene            complement(325..2385)
                     /locus_tag="VFR20_02365"
     CDS             complement(325..2385)
                     /locus_tag="VFR20_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU0229391.1"
                     /translation="MANEFPPHDEVGALRHQLTRERRVRERAESIAKRKLRQLRRQRE
                     AVDLVEATVSTADRASSADEALEFSVNTICQQLRFSVGHRFVLSAAARDDAPRFEPSN
                     VWYCAEPTRFASLQAATNAEKEACMSHGLPGLVLAKGKPIWVSDLARETRFPFPRVQS
                     ALAAGLRTAFAFPVQECGKVTAILEFFTEGRSGASPALLHALDRIGTQLGLTMERQRF
                     RDRLIYEAFHDPLTTLPNRAKLLERLQLILEHSKRRPQYRFALLFIDLDRFKWVNDSL
                     GHAYGDALISEASRRLAACVRREDLVARNISALEAGFMGHIVARLGGDEFTVILEDIR
                     GASDAMRVAQRIQEAFSAPFVLANQNLYISASIGIALNTADHETAQDVLRDADIAMYR
                     AKARGRGCHELFDQAMGAHAKKALKLETELHEAVERREFQLLYQPFLSVDDSTIRGFE
                     ALLRWRHPERGLLLPDQFLPTIEETGLIRTIGRWVREEACRQARRWQNAFPSDPPLAM
                     SINVSASEFNEEGFVDHLSCVLNATGLPPPSLILELTETVAMANLEQAQRVFAQIKEL
                     GVRLSLDDFGTGHSSLSSLRRLPIDVLKVDRSFVNGLDCNEGNQHVLEMIVMLARAFN
                     MRIVAEGPETVEEFTYLKRLGCHYVQGFYFHRPLSEAAATAELRKQRQRSDEAPDADI
                     HTLS"
     gene            complement(2637..2900)
                     /locus_tag="VFR20_02370"
     CDS             complement(2637..2900)
                     /locus_tag="VFR20_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229392.1"
                     /translation="MLGRTFSRYGVAVLLATLVAGCSLFGAKTSKPDGRAKSWGYDSV
                     CKRNPGQCMYKGHYEPGEREYAEQEAKRLNQASLDRLRHSSGY"
     gene            3117..3917
                     /locus_tag="VFR20_02375"
     CDS             3117..3917
                     /locus_tag="VFR20_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0229393.1"
                     /translation="MDLGIAGKTALVCGASKGLGLGCAQALVREGVNVVIVARGAEPL
                     RVATRSLQAIAATNEFATPSVHHVAADITTDEGRATVFAEHKDFDIVVTNAGGPPPGD
                     FHDWDRRTWTAALEANMLTPIELIKATVDGMARRGYGRIVNITSSSVKAPIDILGLSN
                     GARSGLTGFIAGLARSDIAAQGVTINNLLPGAFDTDRLRHTMEAAAQKSGQKTATVLE
                     ARKRTIPARRFGTPAEFGAICAFLCSMQAGYLTGQNILLDGGAYPGTY"
     gene            4058..4993
                     /locus_tag="VFR20_02380"
     CDS             4058..4993
                     /locus_tag="VFR20_02380"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU0229394.1"
                     /translation="MKSLISTLLTVLAVGIWVPAWAAQPLLTPAELHAIDHDPGVRII
                     DIRPGKAYAAGHIPGAISAPYHQWRGPANDPGKLPPESVLTTLVRHLGLNGDTHAVVV
                     SSGKDSTDFGGSARVYWTLKYLGLTQLSILNGGLKAWADAGLPEDRVIPTVAPSQYVA
                     HLDNSLIATTQDVAKQVGNAQTRLIDARPHKFYLGEVKAPTALVPGTIKDAINLENGK
                     WFEPGTAIFVSPDKARRIAALELSQPKQETISFCNTGHWAATDWFALSEVVGIQNVRL
                     YPASLAEWTHDAKLPMQNVPGRGEQILMKLKGLLG"
     gene            5002..6150
                     /locus_tag="VFR20_02385"
     CDS             5002..6150
                     /locus_tag="VFR20_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HEU0229395.1"
                     /translation="MAALPTTTSGLGAVPVAHDERVRWRGRVLLALAIAACIVLLAWF
                     VSLRQALLFLVGLGMGATLAGTRFGFTTGWRNFIERRDPSGILAQLLLIALAAEICIP
                     MLAAHPADLRAALGPPSISLLIGAFVFGACMQIADGCGSGTLYKAGLGIPLNTVILPV
                     FAIGSFAGSATLERWLALGSIAPYGLVTHLGGAEALGVTLAGLVILAAIVLWRSDPSA
                     CRLTRKLIIGAVLLAILAALNLAIAGQPWGIVYGFGLWGAKLATAAGLFDPTTNAFWR
                     EAVNTTTLHQSVFLDVTSITDIGILAGALWIAVGKPRSESRPLTGKQWLIGIVAGFLL
                     GYSSRLAFGCNIGAMVSGISTGSVHGWIWVAMAYLGSLLGVRLRRRYF"
     gene            6164..6328
                     /locus_tag="VFR20_02390"
     CDS             6164..6328
                     /locus_tag="VFR20_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229396.1"
                     /translation="MRKPLLITLFWAALIAFLAWDWAHSAPIDLRHEPPVIALGSGQA
                     PTGGHCASTF"
     gene            complement(6371..7012)
                     /locus_tag="VFR20_02395"
     CDS             complement(6371..7012)
                     /locus_tag="VFR20_02395"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU0229397.1"
                     /translation="MFPQRLTDGYQSFLHGRLAHERARYQELAEKGQHPEVMIIGCGD
                     SRVAPETIFDAGPGEMFVVRNIAALVPPCEADVEGSYHGTSAAIEFGVNALRVKHLVV
                     MGHASCGGVAAFANKAEPLTKRDFIGKWMSQMGPVAERVGPPMGDHAGWIRKMEWGIV
                     ELSLQNLMTFPAVRERVQEGSLTLHGAYFGVATGVLFVRDPVSGEFRPYAEKA"
     gene            7217..8275
                     /locus_tag="VFR20_02400"
     CDS             7217..8275
                     /locus_tag="VFR20_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU0229398.1"
                     /translation="MNSNNIQFQAFLLLLIIVTVAFGWLLRPYYGALFWGAALAILFM
                     PLHRRVLRGFGGRSNLAALTSVVLCVLIVIIPLIFITGALVQEGAVLYHQISAGKIDL
                     GQSFEQAQHALPAWMRDELARFGLGDMESLKSTLSSGAVKASKFLATQAFNVGQNTFE
                     FVISLGIMLYLLFFLLRDGPRLGQHMKLLIPLNDEHKHHLFSKFTTVVRATVKGNVVV
                     AATQGLLGGIIFWVLGINGALLWGVLMGFLSLLPAVGAALIWFPISVFFLLTGAYWKG
                     TILILFCVLVIGLVDNVMRPLLVGKDTKMPDYVILISTLGGLTLFGLNGFVIGPLIAA
                     LFMACWDLFPSAIRMSGD"
     gene            8334..8798
                     /locus_tag="VFR20_02405"
     CDS             8334..8798
                     /locus_tag="VFR20_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU0229399.1"
                     /translation="MNASAGTSSAHPPAPHDVAPDLFRNMVEQAGDAIIFIDRDGAIQ
                     AWNRGAQNAFGYSADEARHLSLDVIIPENLRQAHWVGFQKAVDSGHTQHGDRVLTTKA
                     IHKDGHTVYVDMHFCLVKDDSGRVAGALAIGRDCTERYLAEKARRKAAQQQG"
     gene            complement(8916..9461)
                     /locus_tag="VFR20_02410"
     CDS             complement(8916..9461)
                     /locus_tag="VFR20_02410"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEU0229400.1"
                     /translation="MKIFTLEHHPVSYFADFVLYPFAIMAGLAVLLLYAPDIHWPLLL
                     AGVVGFVVWSLAEYILHRFVLHGVQPFKRWHEEHHKRPFALIGTSTFASLVLFVILVY
                     LPVAFVFGRWPALAVTLGVISGYLFYVTVHHASHHWKARPGSWLQARKLAHARHHMSG
                     MHGWYGVTTSFWDHVFFTGRR"
     gene            9699..9774
                     /locus_tag="VFR20_02415"
     tRNA            9699..9774
                     /locus_tag="VFR20_02415"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9732..9734,aa:Thr,seq:cgt)
     gene            complement(10013..10231)
                     /locus_tag="VFR20_02420"
     CDS             complement(10013..10231)
                     /locus_tag="VFR20_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEU0229401.1"
                     /translation="MKTAQRMLIGTVLATALLGLSGCSGMSRQGRNTAIGAGVGAVGG
                     AVLTGGSTLGTLGGAAVGGIVGHEATKK"
     gene            complement(10405..11550)
                     /locus_tag="VFR20_02425"
     CDS             complement(10405..11550)
                     /locus_tag="VFR20_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEU0229402.1"
                     /translation="MAIDAATISTNDKSLIEGIREWVMLESPSHDIERVGKMMVLAGE
                     QARRMGLRTDLIDLGAESAKMLHVTNRQPGDVSPGLLIIAHLDTVHPVGTLRVNACRQ
                     EGDRLYGPGVYDMKAGAYLALCALGEAARNGGTALPVDFLFSPDEEIGSPASRSHIER
                     FAGRAHCVLVTEPARPNGGRCVTARKGTGVVKVKIIGRPAHAGMQHENGRSAIKEMAH
                     QVLALEAMTDYARGITVSVGTIRGGTTTNVVPSECEAIVDFRVPDAEAAEFLLARVRA
                     MKAVDPDVTLSIDAALNRPPMARDENTAALFATASDCANRAGFSLEEAPMTGGGSDAN
                     FTAALGIPTIDGLGADGDGAHTLKEYVLVSTLAQRLAFWKELLRGLR"
     gene            11664..>13388
                     /locus_tag="VFR20_02430"
     CDS             11664..>13388
                     /locus_tag="VFR20_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEU0229403.1"
                     /translation="MHSSDDKPETAPTRDECLPLFGRVLFLSANAATLRAQLGGADVE
                     LPRALPLRDDISTDEITPMAALAHYDDALGRHPYTGLRVGDETPIALDAIVTAGFTVT
                     VAGKRYGKGSSREHSPMAEKLAGIRLVVAESFERLYRQNADNIGLLTSTDFSLIERIQ
                     RGERIALRELLAGRDALASDILACGGLLGFGRRHLSNITAPISQQTHRHAGSRTLFEK
                     IVARHLLSTATTRNNTNPGQGVFVRADWRFIHEYYTGMCAHMLRTAADPTPALHDAAS
                     IVAFEDHTSYVEASAMHRTRGLVQPIRAMCRAHREFVAAHGLRSHRTLTEDEAAQDKG
                     DNAAGISHAMMTEHYALPGQLVVGTDSHTPHSGALGCVAFGVGSTDMANAFITGAVRM
                     TLPESLLIRLEGHLPAGITAKDIVLDLLARPEIRAGLGVGKVFEFVGPALDGLSVDER
                     ATLTNMTAELGGLTGIVAPDSHTVRFLRERRGVEFVLESWMRSDTGAHYAATLRVDCT
                     RLTPMVARPGDPGNGIALKDLPAPVRVDIAYGGSCTAGKRADFDHYYEVLAWGLARGL
                     RVPDSVTLY"
BASE COUNT         2618 a         4336 c         3903 g         2531 t
ORIGIN      
        1 ttgccacgga agcgcgtttg cgcgcgtcat tttttctatg ctatagtttc gcttctttgg
       61 atgttccccg atagctcagt cggtagagcg acggactgtt aatccgcagg tcgctggttc
      121 gagcccagct cggggagcca acaggatcaa gcacttacgc caacctttcc ggttggcgtt
      181 ttgctttgtg gctccattta ttggcccagg gggccttcgg atcacaccgg caacgaggga
      241 aaatggtatc aagttgacgg gcaggcccca cagaccggcc ttgcgctatt gcggcttgtt
      301 ttcacctacg ttgcgggttg agagtcagga gagagtgtgg atgtcggcgt cgggcgcctc
      361 gtcgctccgc tgtcgctgct tgcgcagttc cgccgtggcg gcggcttcac tcagggggcg
      421 gtgaaaataa aagccttgaa cgtaatggca accaagacgc ttcaagtagg tgaattcctc
      481 gacggtttcc ggcccttcgg ccacaatccg catgttgaaa gcgcgcgcaa gcatgacgat
      541 catttcaaga acatgctgat ttccttcatt acagtcaagt ccgttcacaa aagaccgatc
      601 gaccttgagc acgtcgatag gcagacgcct cagggaactc agcgaagagt ggccagtgcc
      661 gaaatcatcc agactcaagc gcacgcccag ctctttgatc tgggcgaaca cccgttgggc
      721 ctgttcaaga ttcgccatcg cgacggtttc agtaagctcc aggatcaggc ttggcggcgg
      781 caggccagtg gcattcaaca cgcagctcaa gtggtcgaca aacccctctt cgttgaactc
      841 gctggccgag acattgatgc tcatcgcgag tgggggatcg gacgggaagg cattttgcca
      901 acggcgcgcc tgtctgcagg cctcttcccg cacccagcgg ccaatggtac gaataaggcc
      961 cgtctcttcg attgtgggca aaaactggtc gggcagcagg aggcctcttt cggggtgccg
     1021 ccatcgcaac agcgcctcga aaccgcggat ggtgctatcg tcgacggaga ggaacggttg
     1081 atacagcagt tggaattccc ggcgctccac cgcctcatga agctcggtct ccagcttcag
     1141 cgcctttttc gcgtgcgctc ccatcgcctg gtcgaatagt tcatgacaac ctcgcccgcg
     1201 cgccttggcg cggtacatgg cgatgtcggc gtcacggaga acatcctggg cggtttcgtg
     1261 gtctgcggta ttgagcgcaa tgccgatact tgccgaaata tagagattct ggttggccaa
     1321 cacgaacggg gctgagaaag cctcttgaat ccgctgagcc acccgcatgg catcgctcgc
     1381 accacggatg tcctcgagaa tgacagtgaa ttcatcgcca ccaaggcgtg cgacgatatg
     1441 ccccatgaag cccgcctcaa gggcggaaat attgcgtgcg accaaatcct cgcgtcggac
     1501 gcacgccgcc agccgccgac tggcctccga gatcagagca tctccgtatg catgccccag
     1561 gctgtcgttg acccacttga aacgatccag atcgatgaac agcaatgcga accggtactg
     1621 cggtcgacgc ttgctgtgct ccagaatgag ttgcaggcgc tcgagcagtt ttgcgcgatt
     1681 gggtaacgtg gtcagaggat catggaatgc ttcgtagatc aaccgatccc tgaaccgctg
     1741 gcgctccatg gtgaggccga gttgagtgcc gatccgatcc agcgcgtgca gcaacgccgg
     1801 actggcgcca ctgcgcccct cagtgaagaa ctccagaatc gcagtcactt tgccgcattc
     1861 ctgtacgggg aaagcgaacg cggtgcgcag ccctgccgcg agggctgact ggacacgcgg
     1921 aaacggaaac cgtgtttctc gcgcaagatc ggaaacccag atcggtttgc cctttgccag
     1981 aacgagcccg ggcaggccgt gactcatgca ggcctctttt tccgcgttgg tggcggcctg
     2041 aagcgacgcg aaccgtgtcg gctcggcgca ataccaaaca ttcgaaggtt caaatctggg
     2101 cgcgtcatcc cgcgccgccg cgcttaagac aaaccgatga cctacggaaa accgcaactg
     2161 ttggcagatg gtgttgacgg agaactcgag cgcctcgtcg gctgagctcg cccgatcagc
     2221 ggttgaaacc gtggcctcga ccagatcgac agcctcccgc tgacggcgca attgcctcag
     2281 tttgcgcttt gcaatggatt ccgcgcgctc acgcaccctg cgctcgcgcg tcaattgatg
     2341 acgcaaagcg ccaacctcat catgaggggg aaattcgttc gccatctcgg tctaggccac
     2401 ccatgtgttc agtctggctg tccatcctgc tggtgccacc tctgatccct gtgcgacgag
     2461 tacaggcaac tctagtcgga atgaaacatt tgtttaagtg attttaataa aaattacttt
     2521 tttacctcat catcgatgca tttttcatat atctgaaaac gatagaataa tagttttttt
     2581 tccataccca gggggaacct ggtgtccgga tcacgccggg catgggtggg gctcggtcaa
     2641 tagccggaac tgtggcgcag cctgtcgagc gaggcctgat tcaggcgttt cgcttcctgc
     2701 tcggcatatt cgcgctctcc tggctcatag tgccctttgt acatgcattg gccggggttc
     2761 cgcttgcata cactgtcgta accccaggac ttggcgcggc catcaggttt tgacgtcttg
     2821 gccccaaaca aggaacaccc ggcaaccaac gtggcaagaa gcactgccac accatatcga
     2881 ctgaacgtac gaccgagcat gttgtctccc ctggatacga tgatcattta tttgtattgc
     2941 ggattatggg cggcggagtg gtacggcaat gtgaacaatc gaaagtggtt gtgtgagcgc
     3001 gcttcgggct tcgccacgcc gaacgcgatt acgattcagg cattgaccaa cagtcctgcg
     3061 cccgccggct gcgtggcccg cagccctctt caatcattta tcaaaggaaa atcgacatgg
     3121 acttgggaat agcaggcaaa accgccctgg tgtgcggcgc gagcaaaggc ctgggcctgg
     3181 gctgtgcgca ggcattggtg cgcgaaggcg tgaatgtcgt catcgtggcg cgcggcgccg
     3241 aaccgctgcg ggtggcaacc cggtcactgc aagcgatcgc ggccaccaat gaattcgcga
     3301 cgcccagtgt ccaccacgtc gccgccgaca tcacgacgga tgaaggccgg gccaccgtgt
     3361 tcgcggaaca caaagacttc gacatcgtcg tcaccaacgc gggcggcccc ccaccgggcg
     3421 atttccatga ctgggatcgc aggacatgga cggcggcgct ggaggcgaac atgctcacgc
     3481 cgatcgaact gatcaaggca accgtggacg gcatggctcg gcgcgggtat ggacgcatcg
     3541 tgaacatcac gtcgagctcg gtcaaggccc ccatcgatat cctcggcctg tccaatggcg
     3601 cgcgcagcgg gttgacaggt ttcatcgccg gactggcacg cagcgacatc gccgcgcaag
     3661 gcgtgacaat caacaacctg ctgccaggcg ccttcgacac agaccggttg cgccacacca
     3721 tggaagccgc ggcgcaaaaa tcgggacaaa aaacagccac tgtattggaa gcacgcaaac
     3781 gcaccatccc ggctcgccgc tttggcactc cggcggagtt cggcgcgatc tgtgcttttc
     3841 tgtgcagtat gcaagccggg tatctgacgg ggcagaacat cctgttggat ggcggagcct
     3901 acccgggaac gtattgaact ccgtgacctg atgaaacgcg cggaactcag atagcccaat
     3961 atcattgaaa aatatataaa aatgaagaac taatattttt ccgtataaaa agattgagga
     4021 acaataggca cttccctgct ttttgccgga gcgccacatg aagtctctga tctcaacgtt
     4081 gctcactgtg ctcgccgtgg gcatctgggt gcccgcatgg gcggcacagc ccctgctcac
     4141 cccggcggaa ctccacgcca tcgatcacga ccccggcgtg cgcatcatcg acatccgccc
     4201 cggcaaagcc tatgccgcgg gccacattcc aggcgcgatc tcggctccct accaccaatg
     4261 gcgcggcccg gcgaacgatc cgggtaaact cccgccggaa agcgtgttga cgacgctggt
     4321 acgccatctg gggctgaacg gggataccca cgccgtggtg gtgtcgtcgg gcaaggactc
     4381 gaccgatttt ggcggctcgg cccgcgtgta ctggacgctc aagtatctgg ggttgacgca
     4441 actctccatc ctcaatggcg gcctgaaagc ctgggccgat gccggcctgc cggaagaccg
     4501 cgtcattccc accgtcgccc cttcccaata cgtcgcgcac ctggacaaca gtctgatcgc
     4561 caccacccag gatgtggcga aacaagtggg caatgcgcag acacggttga tcgacgcccg
     4621 cccacacaag ttctatctgg gcgaagtgaa agcccccacc gcgctggttc ccggcaccat
     4681 caaggacgcc atcaacctcg agaacggcaa atggttcgaa ccaggcacgg ccattttcgt
     4741 ctcgcccgac aaggcgcggc gcatcgcggc gctcgaattg tcccagccga agcaagagac
     4801 catttcattc tgcaacacgg ggcattgggc tgcgaccgac tggttcgcgc tgtccgaagt
     4861 cgtgggcatc cagaacgtgc gcctctatcc cgcctcgctg gccgaatgga cgcatgacgc
     4921 caagctgccc atgcagaacg tgcccggccg cggcgaacag atactcatga aactcaaggg
     4981 cctcctcggc tgagcgacac catggcggca ctccccacga caacctccgg tctcggcgcg
     5041 gtaccggtcg cacacgatga gcgcgtccga tggcgcggcc gggtcttgct ggcactcgcc
     5101 atcgcggcat gcatcgtcct gctcgcttgg ttcgtctcgt tgcgccaggc cttgctgttc
     5161 ctggtgggcc tggggatggg cgcgacactt gcgggcacgc gcttcggctt cacaaccggt
     5221 tggcgtaatt tcatcgagcg acgcgacccc agcggcatcc tcgcccagtt gctattgatc
     5281 gccttggcgg ccgagatctg catacccatg ctcgcggcgc atcccgccga tttgcgcgcg
     5341 gcgctggggc cacccagcat cagcctgttg atcggcgcct tcgtgttcgg cgcctgcatg
     5401 cagatcgccg acggctgtgg atccggcact ttgtacaagg ctggcctggg cattccgctg
     5461 aacaccgtga tacttccggt cttcgccatc ggcagcttcg cgggttccgc caccctggaa
     5521 cgctggttgg cccttggctc gatcgcgcct tacggcctcg tcacccacct gggcggcgcc
     5581 gaagcgctgg gcgtcaccct ggccggcctg gtcatactgg ccgccatcgt gctgtggcga
     5641 tccgacccct cggcctgccg cctgacccgc aaactgatta tcggcgccgt gctgctggcc
     5701 attcttgcgg cgctcaacct cgccatcgcc gggcaacctt ggggcatcgt ctacggcttc
     5761 ggactgtggg gcgcgaaact cgccaccgcc gccggcctgt tcgatccgac caccaatgcc
     5821 ttctggcgcg aggcggtgaa caccaccaca ttgcatcagt ccgtgttcct ggatgtcacg
     5881 tccatcaccg atatcggcat actggccggc gcgctttgga tcgccgtggg caaaccccgc
     5941 tccgaatccc gcccgctgac cggcaaacaa tggctgatcg gcatcgtcgc cggcttcctg
     6001 ctgggctaca gttcccgcct ggcattcggc tgcaacatcg gcgcgatggt cagcgggatc
     6061 tcgaccggta gtgtgcacgg ctggatctgg gtggccatgg cctacctggg ctcactgctc
     6121 ggcgtacgcc tgcgccgccg ctatttctga accgcatgat gccatgagaa agccgctact
     6181 catcacgctg ttctgggccg ccctgatcgc cttcctcgcc tgggactggg cgcatagcgc
     6241 ccccatcgat ctgcgccacg aaccgcccgt gatcgcgctg ggatcaggcc aggcccccac
     6301 cggcggccat tgcgcctcga cgttttaaac gagcagactc gaggcgcaaa ccaccggcaa
     6361 cgaccgtgcc tcaggccttc tcggcgtaag gccgaaattc cccgctgacc ggatcccgca
     6421 cgaacaacac ccccgtcgcc acgccgaaat acgccccatg cagcgtcagg ctgccctctt
     6481 gcacacgctc gcgcacggcg gggaatgtca tgagattctg gagactcagc tccacgatcc
     6541 cccattccat cttccggatc caaccggcgt gatcccccat cggcggcccc acccgttcag
     6601 ccaccggccc catctgcgac atccacttgc cgatgaaatc acgcttggtc aacggttccg
     6661 ccttgttggc gaacgccgcc acccccccgc agctggcatg ccccatcacc accaggtgct
     6721 tcaccctcag cgcattgacc ccgaactcga tcgccgcact ggtgccatgg taggaaccct
     6781 cgacatcggc ctcgcacggc ggcaccagcg ccgcgatgtt gcggaccacg aacatctcgc
     6841 ccgggcccgc gtcgaaaatg gtttccggcg ccacacggga atccccgcaa ccaatgatca
     6901 tgacttccgg atgttggcct ttctcggcca actcctggta gcgcgcgcgc tcatgcgcca
     6961 gacgcccgtg caggaaagat tggtagccat cggtcagtcg ttgtggaaac atgggttctc
     7021 tcttaaaaaa ggaattaaga cacccgattt taaacccacc ggcacacctc accctgaaca
     7081 atcaacagcg atagccttcg cgccccccgc ccgtgaaaca cctgtggcac aatgcccgag
     7141 aaatcaggcc accgcccgat gccgcgtccg cgcggcaaca cggcacggcc accgcgcatg
     7201 gcttccggga gcccagatga acagcaacaa cattcagttc caagcctttc tgctactgct
     7261 catcatcgtc accgtcgcgt tcggctggct gctgcgcccg tactatggcg ccctgttctg
     7321 gggcgcggcg ctggccatcc tcttcatgcc attgcaccgc cgcgtcctga ggggcttcgg
     7381 tggacgctcg aatctcgcgg cactgacgtc ggtggtgctg tgcgtgctga tcgtgatcat
     7441 ccctcttata ttcatcactg gcgcgctggt acaggaaggc gccgttctct accaccagat
     7501 ttcggccggc aagatcgacc tgggccaatc ctttgaacag gcgcagcacg cgctaccagc
     7561 gtggatgcgg gatgaactcg cccgcttcgg gctgggagac atggaaagcc tgaaaagcac
     7621 cctatccagc ggcgctgtga aagccagcaa gttcctcgcc acccaggcgt tcaacgtcgg
     7681 acaaaacacc ttcgaattcg tgatcagcct gggcatcatg ctgtatctgc tgttcttcct
     7741 gctgcgcgac ggcccgcgcc tgggtcagca catgaaactg ctcattccgc ttaacgacga
     7801 gcacaagcat cacctgttca gcaagttcac cactgtcgta cgggccacgg tcaaaggcaa
     7861 cgtcgtcgtg gccgccaccc aagggctgtt gggtggcatc atcttctggg tgctgggcat
     7921 caacggtgcc ctgctgtggg gggtgctgat ggggttcctg tcgttgctgc cggcagtggg
     7981 tgcggcgctg atctggttcc ccatctcggt gttcttcctg ctgacggggg cctactggaa
     8041 aggcaccatc ctcatcctgt tctgcgtcct ggtcatcggc ctggtcgaca atgtgatgcg
     8101 tccgttgctg gtgggcaagg acaccaagat gcccgactat gttatcctga tctcgaccct
     8161 cggcgggttg acgcttttcg ggctgaacgg gttcgtgata gggccgttga tcgcggcgct
     8221 tttcatggcc tgctgggacc tcttcccctc ggccatcaga atgtccggcg actaagttgc
     8281 gcggcaggct attgcgccat gccgccagcc cccaaccgtt aacggactcc cccatgaatg
     8341 cttccgccgg tacctcaagc gcccatccgc ccgcgcccca cgacgtggca cccgacctgt
     8401 tccgcaacat ggtcgaacag gcgggcgacg ccatcatttt cattgatcgt gacggcgcca
     8461 tccaggcctg gaaccgcggc gcacagaacg cattcggcta cagcgccgac gaagcccggc
     8521 acctgagcct ggatgtcatc atcccggaaa acctgcgcca ggcgcactgg gtcggctttc
     8581 aaaaagccgt tgacagcggc cacacccaac acggcgaccg cgtactgacc accaaggcca
     8641 tccacaaaga cggccacacg gtatacgtcg acatgcactt ctgcctggtg aaggacgatt
     8701 cggggcgcgt tgccggcgct ctggccatag gccgcgactg cactgaacgc tacttggctg
     8761 aaaaggcgcg gcgcaaggcg gcgcagcagc agggttgaag aactcttctt tacccggggc
     8821 gccagacatc gccggttcgc cgatcggtgc cctggccggc ttgaaagaat gccattcgcc
     8881 gggaacaggc gctggcctat gcgcggaata gcttgctaac gccggccagt gaagaacacg
     8941 tggtcccaga acgaggtggt caccccgtac caaccatgca tgccgctcat gtggtggcgg
     9001 gcatgcgcca gcttgcgcgc ctgcaaccac gaccctggac gcgccttcca gtgatgtgag
     9061 gcgtgatgca cggtgacata gaaaagatag ccggaaatca cccccagagt gaccgcgagc
     9121 gcgggccaac ggccgaacac aaaagccaca ggcaagtaca ccaatatgac gaacagcacc
     9181 aggctcgcga acgtggaggt gccgatcaac gcgaagggcc gtttgtggtg ttcttcgtgc
     9241 cagcgcttga atggctgcac accatgcaaa acgaaccggt gcaatatata ttcagccaga
     9301 ctccagacca cgaaacccac cacgccggcc agcagtaagg gccagtggat gtcgggcgca
     9361 tacaggagca acaccgccag ccccgccatg atggcaaaag gatagagcac aaaatccgcg
     9421 aaatacgaca ccggatggtg ctcaagcgtg aaaatcttca ttgcaacccc gaaaccgaag
     9481 gtggcgccgg ctcggttccg atcaagtgac cggaccccgc gacgcgacac cttgtgccca
     9541 aaccatctct gcttcgcaat tgtgtatcta caatcatacc tgcgtttcgg cattacacaa
     9601 tagacaacga ataagtcagc cggttccaat tttgcgcggc atggcattgg aaaattcttg
     9661 ggtggtatgt gtataatgtt gagctttcca gaaatcaggc cggtgtagct cagttggtag
     9721 agcagctcat tcgtaatgag aaggtcgagg gttcgactcc tttcaccggc accaatgatt
     9781 tgaagcaatg aaaagccacc tgcaacaggt ggcttttttt atggttctca ttttttatgg
     9841 ctgtcagtaa tagccatggt gggaatgcag catagccatg cgtgggaatg ccgtacatgg
     9901 gcagaacatg ggcagaacat gggcgcagcg gcacacggcg acttgggcca catcgcgcat
     9961 gtctgcgaca agcccgcaca cgccctggcc gccttacctc accccggccg tcttatttct
    10021 tggtggcctc gtgtcccacg ataccgccca cggcggcgcc acccaatgta cccagggtgc
    10081 tgccgcctgt gagcacggct ccaccgaccg cgccgacacc agcgccgatc gccgtgttgc
    10141 gcccttgccg agacattcca ctgcaaccgc tcaagccaag cagcgccgtg gcaagcaccg
    10201 tgccaatcaa cattcgttga gctgtcttca taaaggacct cccgcaaaat aatcgctcca
    10261 ccgtgcggag cctgtctgac tacagtaaca ccaagtcaac aatccctgac aataccaact
    10321 gtaacggtcc tgcgcgaaag acaacttgtt cagtcgagtg tacattgaca cgcgtggacc
    10381 agacgtccgc cgtggtctgc gtgatcaccg cagtccgcgc agcagttcct tccagaaagc
    10441 cagccgctgc gccaatgtgg aaaccaacac gtattccttc aacgtgtgcg cgccgtcacc
    10501 atcggcgccc aacccatcga tggtcggaat gcccagggcg gcggtgaaat tggcatcgct
    10561 gcctccaccc gtcatggggg cctcttccag agaaaagccg gcgcggttcg cacaatcgct
    10621 cgcggtggcg aaaagcgccg ccgtgttctc gtcgcgcgcc atgggcgggc gattcagcgc
    10681 ggcatcgatg gacaatgtga catccgggtc gaccgccttc atggctcgga cgcgggccag
    10741 gaggaactcg gccgcttcgg catccggcac ccggaaatcg acgatggctt cgcattcgga
    10801 cggcacgacg ttggtggtgg tgccaccgcg tatggtgccc acgctgacgg tgatgccgcg
    10861 cgcgtagtcg gtcatggcct ccagcgccag gacctggtgc gccatttcct tgatggcgct
    10921 gcgcccattt tcatgctgca tgccggcatg cgccggccgg ccaatgattt tcaccttcac
    10981 cacacccgtt cccttgcgag cggtgacaca gcgcccgccg ttcgggcgcg cgggttcggt
    11041 caccaggaca caatgcgccc gaccggcaaa acgttcgatg tgcgaccgcg aggcggggct
    11101 gccgatttcc tcatccggcg aaaagagaaa atccaccggc aatgccgtcc cgccattccg
    11161 ggcggcctca cccagcgcgc acaatgcgag atacgcgccc gccttcatgt cgtacactcc
    11221 tgggccgtac agacggtcgc cttcctgacg gcaggcattg acccgcagcg tgcccaccgg
    11281 atgcacagtg tcgaggtggg cgatgatcag caggccgggg ctgacgtcgc ccggctggcg
    11341 attggtcaca tgcagcatct tcgccgattc cgcccccaag tcgatcaggt cggtgcgcaa
    11401 ccccatgcgc cgggcctgtt cgcccgccag caccatcatc ttgcccactc gctcgatgtc
    11461 atgggacggg gattccagca tcacccattc ccggatgcct tcgatgagtg acttgtcatt
    11521 cgtggaaatc gtggcggcat caatagccat ggtcttctcc tgtatcagcc tgaagtgtga
    11581 ggatacactg tgctttctgt actttcgccc accggggcgt ccggaaagac ttggaccacg
    11641 gctcgcggcg accaggaaac atcatgcaca gcagtgacga caagcccgaa acagcaccaa
    11701 cgcgcgacga atgcctcccc ctattcggcc gcgttctatt cctgtcggcc aacgcggcca
    11761 cgctacgtgc gcaactcggc ggagcggatg tcgaactgcc tcgcgcgctg ccgttgcgcg
    11821 acgacatttc cacggacgag atcacgccga tggcggcgct ggcccattat gacgacgcgc
    11881 tgggccgtca cccgtatacc ggcctgcggg tcggcgacga gacgcccatc gcactggatg
    11941 ccatcgtcac cgccggcttc accgtgacag tggccggaaa gcgctacggc aaaggttctt
    12001 cgcgcgagca cagccccatg gccgaaaagc tggcgggcat ccggttggtg gtcgccgaaa
    12061 gcttcgaacg gctctaccgg cagaacgccg acaacatcgg gttgctcacg tccacggatt
    12121 tcagcctgat cgaacgcatc cagcgcggcg agcgcatcgc cctgcgcgaa ttgctggccg
    12181 ggcgtgacgc gctggcgtcc gacattttgg cctgcggcgg actgctgggg ttcgggcgcc
    12241 ggcatctgag taacatcaca gcccccatct cgcagcaaac acatcggcac gccgggtcgc
    12301 gcacgctgtt cgagaaaatc gtcgcgcggc acctgctgag caccgcgacc acgcggaaca
    12361 acactaatcc cgggcaaggg gtgttcgtgc gcgccgactg gcgtttcata cacgagtact
    12421 acaccggcat gtgcgcccac atgctgcgga cggcggccga tccgacgccc gccttgcatg
    12481 acgccgcatc catcgtcgcg ttcgaggatc acacgtcgta tgtggaggcc agtgccatgc
    12541 atcgcacgcg cggcctggtg caacccatac gggccatgtg ccgcgcccat cgggaattcg
    12601 tcgcggcgca cggcctgcgt tcgcaccgca cgctcaccga agacgaggcc gcgcaagaca
    12661 aaggcgacaa cgcggccggc atctcgcacg ccatgatgac ggaacactac gccctgcccg
    12721 gccaacttgt cgttggcaca gactcgcaca cgccgcacag cggcgccctg ggttgcgtcg
    12781 cctttggtgt gggctccacc gacatggcca acgcgttcat caccggcgcg gtgcgcatga
    12841 ccttgcccga atcgttgttg atccggctcg aaggccacct gccggcgggc atcaccgcca
    12901 aggacattgt gctggatctt ctggcgcggc cggagatacg cgccggcctg ggcgtaggca
    12961 aggttttcga attcgtgggg cctgccctcg acggtttgtc ggtggacgaa cgcgcgacgc
    13021 tcaccaacat gacggcggaa ctcggcggtc tcaccggcat cgtggcgccg gactcccaca
    13081 ccgtgcgctt cctgcgggaa cggcgcggcg tggaattcgt cttggaatcg tggatgcgca
    13141 gcgataccgg tgcccattac gcggcgacgc tgcgggtgga ctgcacgcgg ttgacgccga
    13201 tggtggcccg tcctggcgac ccgggcaacg gcatcgcgct caaggatctg ccagcgcccg
    13261 tgcgcgtgga catcgcctac ggcggatcat gcacggccgg gaaacgcgcc gacttcgacc
    13321 actactatga agtgctggca tgggggcttg cgcgcggcct gcgtgtaccg gattcggtca
    13381 ccctgtac
//
LOCUS       DASTNK010000031        13017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1501349, whole genome shotgun sequence.
ACCESSION   DASTNK010000031 DASTNK010000000
VERSION     DASTNK010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13017
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501349"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..157
                     /locus_tag="VFR20_02435"
     CDS             <1..157
                     /locus_tag="VFR20_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU0229404.1"
                     /translation="LPKDKEMVLPGDNVSMTVKLIAPIAMEEGLRFAIREGGRTVGAG
                     VVASIIA"
     gene            223..534
                     /gene="rpsJ"
                     /locus_tag="VFR20_02440"
     CDS             223..534
                     /gene="rpsJ"
                     /locus_tag="VFR20_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU0229405.1"
                     /translation="MKSQKIRIRLKAFDYKLIDQSAAEIVDTAKRTGAVVRGPVPLPT
                     RIRRYDVLRSPHVNKTSRDQFEMRTHQRLMDIVDPTDKTVDALMRLDLPAGVDVEIAL
                     Q"
     gene            854..1543
                     /gene="rplC"
                     /locus_tag="VFR20_02445"
     CDS             854..1543
                     /gene="rplC"
                     /locus_tag="VFR20_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU0229406.1"
                     /translation="MSNPTPTPAAFRLGLVGRKVGMTRIFTEEGESIPVTVLDVSNNR
                     VAQVKSPEVDGYAAVQLVYGTRRASRVTKAQAGHYAKAGVEAGSILKEFRLDPARAAE
                     FSAGAVVAVESVFEAGQKVDVTGTTIGKGFAGTIKRHHFKSQRASHGNSRSHRVPGSI
                     GQAQDPGRIFPGKKMSGHLGDDTRTVQNLDVVRVDAERGLLLVKGAVPGHKNADIIVR
                     PAIKTPAKKGA"
     gene            1547..2167
                     /gene="rplD"
                     /locus_tag="VFR20_02450"
     CDS             1547..2167
                     /gene="rplD"
                     /locus_tag="VFR20_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU0229407.1"
                     /translation="MELKLLNDQGQSSSTVAAPDTVFGRDFNEALVHQIVVAYQANAR
                     SGNRAQKGRDTVKHSTKKPWRQKGTGRARSGMTSSPIWRGGGRAFPNSPEENFSQKVN
                     KKMYRAGIRAILSQLAREDRIAVVDSFQLESPKTKVAATKLKSMGLESVLIITDAVDE
                     NVYLATRNLAHVAVVEPRYADPLSLIHYKKVLITKPAIAQLEEMLG"
     gene            2164..2460
                     /gene="rplW"
                     /locus_tag="VFR20_02455"
     CDS             2164..2460
                     /gene="rplW"
                     /locus_tag="VFR20_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU0229408.1"
                     /translation="MNTERLMQVILAPVVTEKATFVAEKNQQVAFRVVPDATKSEIKA
                     AVELLFKVEVEDVQVLNTKGKEKRFGRFMGRRNNVRKAYVSLKDGQELDFTEVN"
     gene            2461..3288
                     /gene="rplB"
                     /locus_tag="VFR20_02460"
     CDS             2461..3288
                     /gene="rplB"
                     /locus_tag="VFR20_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU0229409.1"
                     /translation="MALVKVKPTSAGRRGMLKVVTPGLHKGAPFAGLLEKKTRGSGRN
                     NNGHVTVRHRGGGHKQHYRVVDFRRNKDGIVAKVERLEYDPNRTAHIALVCYADGERR
                     YIIAPKGLAVGASVVSGAEAPIRAGNALPIRNIPVGTTVHCIEMLPGKGAQLARSAGA
                     SAVLMAREGVYAQVRLRSGEVRRVHIDCRATIGEVGNEENSLRQIGKAGAMRWRGIRP
                     TVRGVAMNPIDHPHGGGEGRTGEGRVPVSPWGTPTKGFKTRRNKRTDTMIVQRRKRK"
     gene            3301..3576
                     /gene="rpsS"
                     /locus_tag="VFR20_02465"
     CDS             3301..3576
                     /gene="rpsS"
                     /locus_tag="VFR20_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU0229410.1"
                     /translation="MSRSIKKGPFVDAHLIKKVDAAVEGKDKKPIKTWSRRSTILPEF
                     IGLTIAVHNGRQHVPVYINENMVGHKLGEFALTRTFKGHAADKKAKR"
     gene            3580..3909
                     /gene="rplV"
                     /locus_tag="VFR20_02470"
     CDS             3580..3909
                     /gene="rplV"
                     /locus_tag="VFR20_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018026679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU0229411.1"
                     /translation="METTAIIRGVHISAQKTRLVADLIRGKSVAQALNILTFTPKKAA
                     GILKRALESAIANAEHNDGADIDELKVTTVYVDKGQSMKRFSARAKGRGNRIEKQTCH
                     IVVKVGV"
     gene            3919..4722
                     /gene="rpsC"
                     /locus_tag="VFR20_02475"
     CDS             3919..4722
                     /gene="rpsC"
                     /locus_tag="VFR20_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU0229412.1"
                     /translation="MGQKIHPIGFRLAVNRNWNSRWYAGDKAFGGMLGEDIRVREYLK
                     KKLKSASVGRVVIERPAKNARITIYSARPGVVIGKRGEDIESLKTDLQRLMGVPVHVN
                     IEEIRKPETDAQLIADSIAQQLEKRIMFRRAMKRAMQNAMRLGAQGVKIMSSGRLNGI
                     EIARTEWYREGRVPLHTLRAIIDYGTAEAQTTYGIIGIKVWVYKGDMLPNGEMPPETA
                     APPRDEERRPRRAPRGERPDGRRPAGGRGRGPRKPAAAASTDAPAPEGE"
     gene            4725..5141
                     /gene="rplP"
                     /locus_tag="VFR20_02480"
     CDS             4725..5141
                     /gene="rplP"
                     /locus_tag="VFR20_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU0229413.1"
                     /translation="MLQPSRRKYRKEQKGRNTGLATRGALVSFGEYGLKATGRGRLTA
                     RQIEAARRAINRHIKRGGRIWIRIFPDKPISQKPAEVRMGNGKGNPEYWVAEIQPGKV
                     LYEMEGVSEDIAREAFRLAAAKLPIATTFVSRHIGA"
     gene            5151..5342
                     /gene="rpmC"
                     /locus_tag="VFR20_02485"
     CDS             5151..5342
                     /gene="rpmC"
                     /locus_tag="VFR20_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU0229414.1"
                     /translation="MKASELRSKNATELKEELDSLLRAHFNLRMQRATQQLSNTSQLG
                     KVRRDIARVRTLMTETAGK"
     gene            5344..5625
                     /gene="rpsQ"
                     /locus_tag="VFR20_02490"
     CDS             5344..5625
                     /gene="rpsQ"
                     /locus_tag="VFR20_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU0229415.1"
                     /translation="MTETQVTAPAKRQRTLIGKIVSNKMDKTVVVRVERRVKHPVYGK
                     IVMRSNKYKAHDETNQYNEGDTVEIAEGRPISRDKSWTVVRLIEAARVI"
     gene            complement(5765..6043)
                     /locus_tag="VFR20_02495"
     CDS             complement(5765..6043)
                     /locus_tag="VFR20_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEU0229416.1"
                     /translation="MPKLKPGTILPTPEEDIRIDRGIAADPDAMEFGEQEAHHARRMG
                     RPPLAAPKIAVTVRYDQDVLDAFRATGDGWQTRMNAALREWLRDHKAA"
     gene            complement(6030..6296)
                     /locus_tag="VFR20_02500"
     CDS             complement(6030..6296)
                     /locus_tag="VFR20_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEU0229417.1"
                     /translation="MQISFDPAKDDGNLRKHGVSLALAVRLEWETALCWDDGRRDYGE
                     PRQVALGYIGQRLYSVVFVDRDEVRRIISLRKANSREVRRYAET"
     gene            6520..6915
                     /locus_tag="VFR20_02505"
     CDS             6520..6915
                     /locus_tag="VFR20_02505"
                     /inference="COORDINATES: protein motif:HMM:NF017355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU0229418.1"
                     /translation="MSDLLEYKGYQGSVGFSAEDHCFFGKIELINDLISFDGSGVDEI
                     TAAFHQAVDDYLAFCQKTRAQPNQPFKGSFNVRVTPALHWRTAVEVKKRGITLDQFIG
                     QSIERMLDGDIEVHDCINPVRTKAPAHAS"
     gene            complement(6993..8462)
                     /gene="phnY"
                     /locus_tag="VFR20_02510"
     CDS             complement(6993..8462)
                     /gene="phnY"
                     /locus_tag="VFR20_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006407230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde dehydrogenase"
                     /protein_id="HEU0229419.1"
                     /translation="MGPTNTNGVSQDSVIDEVRVEAMRIAGERVHRDETIDVCYPYTG
                     EVIATVPKATLDDVRRAFRIAKQYQSKLTRADRYNILMRARDLIAARRDEIARLITLE
                     SGLCLMDTLHEVGRASDVLLFSANQALIDDGQVFSCDLTPHGKQRKVYTMRQPLLGAI
                     SAITPFNHPLNQVIHKVAPSIATNNRMVLKPTEKTPLAAILLADILYEAGLPPEMLSV
                     ITGDPKDIADEMLTNPDVDLVTFTGGVAIGKYIAAKAVYKRQVLELGGNAPFIIMEDA
                     DLAKAAAMTAAGSYKNSGQRCTAVKRIFVQESVAKEFGELLMKETKALKFGDPLDPTT
                     EIGTVIDEEAAMRFESLVINAVEHGAELLYGNKRVGAQYCPTLVDKVRPEMDLIKYET
                     FGPVSPLTRFKTIDEVIGLVNASPYGLSAGVYTNRMDYITRFISELQVGTVNICEVPG
                     YRLELTPFGGIKDSGLGYKEGVIETMKGYTNVKTYSLPW"
     gene            complement(8595..9167)
                     /locus_tag="VFR20_02515"
     CDS             complement(8595..9167)
                     /locus_tag="VFR20_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226784.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HEU0229420.1"
                     /translation="MTTTPPPGRFPIPNVSDLPDDIRERILAVQEKSGFIPNVFLVLA
                     HRPDEFRAFFTYHDALMDKPGNLSKAEREMIVVATSGANQCQYCVVAHGAILRIRAKN
                     PLIADQVAVNYRKADITPRQKAMLDFAMKVSASAYASSDDDMAVLHGHGFDDEDIWDI
                     AAIAAFFGLSNRLANVMNMRPNDEFYMMGR"
     gene            complement(9282..10793)
                     /locus_tag="VFR20_02520"
     CDS             complement(9282..10793)
                     /locus_tag="VFR20_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEU0229421.1"
                     /translation="MKFLIARMNHETNTFSPVPTPLSAFGIDGPTYGADAYAENHGGR
                     TAMSAYIDLAEAAGADIVTPVSATANPSGPVAADAYNTLCQRIIDAAQDCDALMLDLH
                     GAMVAENSQDGEGDLLERLKTRHPDKPIAVALDLHGNVTQKMVDNADVIISFKTYPHV
                     DMYETGEHAGRIFLDLLAGKTTAAIAWERPPLMSHTLKSADLSPAMHAAIDAARAAEK
                     EEVLAVSILAGFSLADIPAPCVSVVVVGRDAESAQRVARRIAGQIWADREGFVYTSPP
                     LAESINIAAELAELSGIQSEGCTNPKGPVLLLDHSDNCMSGGTCDTMDVLQEALAQGL
                     GNIGVGPLCDPEAVALMRHAGEGATITVKLGNKRALEKLGIVKKPVELTGVIKKLTNG
                     EYIITGPTYTGLKCSMGDTALFACGDLEIVVTERTQEPWDLGVFTSVGLDPTQKDYLL
                     LKSRMYCRPVFFPIARGFVECDSAGVTSSNYGLFPFKNVQRPVYPLDAETKWP"
     gene            complement(10856..12118)
                     /locus_tag="VFR20_02525"
     CDS             complement(10856..12118)
                     /locus_tag="VFR20_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEU0229422.1"
                     /translation="MNAPDKLETSPRIDGQRLWRSLMALADIGATPKGGVCRLALTEL
                     DKQGRDLFIGWAKDAGCEVRIDAIGNIFARRAGRDPSLPPVLTGSHIDTQPTGGKFDG
                     NYGVLSGLEVLRTLNDQGIQTDAPIEVVVWTNEEGSRFVPVMMGSGVFAEAFTLDHAL
                     GQQDTEGVSVAQALEAIGYAGATPCRPFPIKAYFEAHIEQGPILEARDDVIGSVTGAL
                     GQRWYDVTLTGMEAHAGPTPMALRKDALLAAAGLIQAVNRIALDHAPHGRGTVGWIDN
                     YPNSRNVIPGTVRLSVDLRAASDDVLDSMNTAITQACHDASRQGVEVHLEQVVYFPPQ
                     PFAPALIDAVRTAAQQLGFSHTDIVSGAGHDAVYVARVAPTAMIFIPCKDGISHNEIE
                     DAKPEHITAGANVLLQAMLAQAGIVPRG"
     gene            complement(12253..12954)
                     /locus_tag="VFR20_02530"
     CDS             complement(12253..12954)
                     /locus_tag="VFR20_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0229423.1"
                     /translation="MMLQVRDIHSYYDKSHVIQGVSLEVRDGELVTLLGRNGAGKSTT
                     LKSIAGLVTPANGQVLFEGRDITRLAPHRIAGQGLCLVPEHRGIFRLLTVEENLNLGR
                     KKHSPWSLQDIYRIFPRLRERRRNGGAQLSGGEQQMLAIGRALMNAPRLLMLDEPVEG
                     LAPVIVEEIVDQLHQIKSQGIAILLVEQNLQVCTQLAERHYIIEGGQIVYTGDNAAFI
                     RDTHVHDQYLGVGAD"
BASE COUNT         2472 a         3907 c         4179 g         2459 t
ORIGIN      
        1 cctgccgaaa gacaaggaga tggtgctgcc gggtgacaac gtgtcgatga cggtgaagct
       61 gattgcgccg atcgccatgg aggaagggct gcgcttcgcc atccgcgagg gtggccgcac
      121 ggtgggcgcc ggcgtcgtcg cctccatcat cgcgtaaaac gcggcatccc gcctgggcgg
      181 gcggcatgtc gcccccgcct tgttcgttct ttaggaaaca ccatgaaaag tcagaaaatc
      241 cgtatccgcc tgaaagcgtt cgactacaaa ctgatcgacc agtcggcggc cgagatcgtc
      301 gatacggcca agcgcactgg cgcggtggtg cgcggtccgg ttccgctgcc cacccgcatc
      361 cgtcgctacg atgtgctgcg ttcgccccac gtgaacaaaa cgtcgcgtga tcaattcgaa
      421 atgcgcacgc accagcggct catggatatc gtcgacccca ccgacaagac cgtcgacgcg
      481 ctgatgcgcc tcgacctgcc ggctggtgtg gatgttgaaa tcgcgctgca gtaacggaaa
      541 gaaggcggga tcctgatgtc ccgaaagaaa caaagcgcca tgcgtatgcg tggcgctttg
      601 tttttggaca tcacccacac cgcattgcgc cgggaggccc cggggtacgg cgtgggcaag
      661 ccgaaaaatg tggcaccgct tgcctgacgc ctggaaataa tgctaaacta cttagcttga
      721 ccgtaaaatc ggtttaatgg ctatgcgccg ctcgttgggt ggcgttgcca tgtgaagtaa
      781 gcaagaaggg cttggccctt tgttttttgc gcaaattagc cccgaccaat cgcagtcggg
      841 aatggagaaa acgatgtcga acccgacacc tacgcccgcc gcgttccggc ttgggcttgt
      901 agggcgcaag gttggcatga cccgcatttt taccgaggaa ggtgaatcca tcccggttac
      961 cgtgctggac gtgtcgaaca accgtgtcgc ccaggtgaag tcgcccgaag tcgacggcta
     1021 cgcagccgtg caactagtct acggcacccg tcgcgcttcg cgcgtgacca aggcccaggc
     1081 cgggcactat gccaaagccg gcgttgaagc cggcagcatc ctcaaggaat tccgtctcga
     1141 ccccgcccgc gccgccgaat tctcggcggg tgccgtcgtg gccgttgaaa gcgtgttcga
     1201 agcaggccag aaggtcgacg tgaccggtac caccattggt aagggtttcg ccggcacgat
     1261 caagcgccac cacttcaaat cgcagcgcgc ctcgcacggt aactcgcgtt ctcaccgcgt
     1321 gccgggctcc attggccagg cgcaggatcc ggggcgtatt ttcccgggca agaaaatgtc
     1381 cggccatctg ggcgacgaca cccgcacggt gcagaacctc gatgtggtgc gtgtcgacgc
     1441 cgagcgcggc ctgctgttgg tcaagggcgc tgtcccgggc cacaagaacg ccgacatcat
     1501 cgtccgcccg gccatcaaga cgccggccaa gaaaggagcg taagacatgg aactcaagct
     1561 cctgaacgat cagggacaat cgtcctccac cgttgccgcg cccgatacgg ttttcggccg
     1621 cgacttcaac gaagcgctgg tgcaccagat cgtcgtcgcc taccaggcca atgcccgtag
     1681 cggcaaccgt gcccagaagg gccgcgacac ggtcaagcac tcgaccaaga aaccttggcg
     1741 ccagaaaggc acgggccgcg cccgttccgg catgacgtcc tcgccgatct ggcgtggggg
     1801 tggtcgcgcg ttcccgaact cgcctgaaga aaacttcagc cagaaggtca acaagaaaat
     1861 gtatcgtgcg ggtatccgcg cgatcctgtc gcagctggct cgcgaagacc gcatcgccgt
     1921 ggtcgattcc ttccagctcg aatctcccaa gaccaaagtg gccgccacca agctcaagag
     1981 catggggctg gagtcggtgc tcatcatcac cgacgctgtc gacgaaaacg tctacctggc
     2041 cacacgcaat ctggcgcatg tggctgtcgt cgaaccgcgt tatgccgatc cgctgtcgct
     2101 gatccactac aagaaagtgc tgatcaccaa gcccgcgatc gctcagctcg aggagatgtt
     2161 gggatgaata ccgaacgtct gatgcaagtc attctcgcgc cggtcgtgac tgaaaaagcc
     2221 acgttcgtcg ctgaaaaaaa ccagcaagtc gctttccgcg tcgtacctga cgccaccaag
     2281 tccgaaatca aggcagccgt cgaactcctg ttcaaggtcg aagtcgagga cgtccaggtg
     2341 ctcaacacca agggcaaaga aaagcggttt ggtcgtttca tgggtcgtcg caacaacgtg
     2401 cgcaaagcat acgtgtcgct caaggacggc caggaactcg actttacgga ggtgaactaa
     2461 atggcactcg taaaagtcaa gccgacctcg gccggtcgtc gcggcatgct caaggtggtc
     2521 acacccggcc tgcataaagg cgcgccgttc gctggcctgc tcgaaaagaa aacgcgcggc
     2581 tcgggccgca acaacaatgg ccacgtcacc gtgcgccatc gtggtggcgg tcacaagcag
     2641 cactaccgtg tcgtcgactt ccgtcgcaac aaggacggca tcgtcgccaa ggttgaacgt
     2701 ctggaatatg accccaaccg caccgcgcac atcgccctgg tctgctacgc cgacggcgaa
     2761 cgccgctaca tcatcgcgcc gaagggcctg gcggtgggcg cgtccgtggt gtcgggcgcc
     2821 gaggcgccca tccgcgccgg caacgcgctg cccatccgca acattccggt cggcaccacg
     2881 gtgcactgca tcgagatgct gcctggcaag ggcgcccagt tggcgcgttc ggccggcgcc
     2941 tccgccgtgc tgatggcccg cgaaggtgtg tacgcccagg tgcggctgcg ctccggtgaa
     3001 gttcgccgcg tgcacatcga ttgccgcgcc accattggtg aagtcggcaa cgaagaaaac
     3061 agcctgcgcc agatcggcaa ggccggcgcc atgcgctggc gtggcatacg cccgacggtt
     3121 cgtggcgtgg ccatgaaccc gatcgaccac ccgcacggtg gtggtgaggg ccgcactggc
     3181 gaaggccgcg tgccggtcag tccatgggga accccgacca agggtttcaa aacccgccgc
     3241 aacaaacgga cagataccat gattgtccaa cggcgcaagc gcaagtaagg ggcgaacaac
     3301 atgtcacgtt cgatcaaaaa aggcccattc gtcgatgcgc atctgatcaa aaaggtcgat
     3361 gcggccgtcg agggcaaaga caagaagccg atcaagacct ggtcgcgccg ttccaccatc
     3421 ctgcccgagt tcatcgggtt gaccattgcc gtgcacaacg gccgccagca cgttcccgtg
     3481 tacatcaacg agaacatggt cggtcacaag ctcggcgagt tcgcgctgac ccgcacgttc
     3541 aagggtcacg ccgcggacaa aaaggccaag aggtaagcga tggaaactac tgctattatt
     3601 cgtggcgtcc acatttcggc ccagaagaca cgcctggtcg cggatctcat ccgcggcaag
     3661 tcggttgccc aggcgctgaa catcctgacg ttcaccccca agaaagccgc gggtatcctc
     3721 aagagagcgc ttgagtccgc gatcgccaac gccgagcaca acgacggtgc cgacatcgac
     3781 gagctcaagg tcaccacggt ctacgtggac aagggccagt cgatgaagcg gttttcggcg
     3841 cgcgccaagg gccgcggcaa ccgcatcgaa aagcagactt gccacattgt ggtcaaggtc
     3901 ggcgtttaag gagtcgcaat gggacagaaa atccacccga ttgggttccg cctcgcggtc
     3961 aaccgcaatt ggaattcgcg ctggtacgcc ggtgacaagg cgttcggcgg catgctgggc
     4021 gaagacatcc gcgtgcgcga atacctgaag aagaagctca agagcgcttc cgtgggccgc
     4081 gtcgtcatcg aacgcccggc caagaacgcg cgcatcacca tttactcggc tcgtccgggc
     4141 gtggtgatcg gcaagcgtgg cgaagatatc gagagcctca agaccgacct gcagcgcctc
     4201 atgggcgtgc ccgtgcacgt caacatcgag gaaatccgca agccggaaac cgacgcacaa
     4261 ctgatcgccg attccatcgc gcagcagctc gaaaagcgca tcatgttccg ccgcgccatg
     4321 aagcgcgcca tgcagaacgc catgcgtctg ggcgcccagg gcgtcaagat catgagttcc
     4381 ggccgcctga acggcatcga aatcgcccgc actgaatggt atcgcgaagg ccgcgtgcct
     4441 ttgcataccc tgcgcgccat catcgactac ggcacggccg aggcgcagac cacgtacggc
     4501 atcatcggta tcaaggtctg ggtctataag ggcgacatgc tgcccaacgg cgaaatgcct
     4561 ccggaaacgg ccgccccccc gcgtgacgaa gaacgtcgtc cgcgccgtgc gccccgtggc
     4621 gagcgtcccg acggccgccg ccccgccggt ggccggggtc gtggcccccg caagccggcc
     4681 gctgccgcgt ccaccgacgc gccggcgcct gaaggagaat aaacatgctg caaccgtcac
     4741 gcagaaaata tcgtaaagag cagaaaggcc gcaacaccgg tctggcgacc cgcggtgcgt
     4801 tggtgtcgtt cggcgaatac ggtctgaagg ccaccggtcg cgggcgcctc acggcgcgcc
     4861 agatcgaagc cgctcgtcgt gccataaacc ggcacatcaa gcgcggtggc cgtatctgga
     4921 tccgtatctt ccccgacaag cccatttcgc agaagcccgc cgaagtccgt atgggtaacg
     4981 gtaagggtaa tccggaatac tgggtcgctg aaattcaacc cggcaaggtc ctgtacgaaa
     5041 tggaaggcgt cagcgaagac atcgcgcgcg aagcgtttcg cctggccgcc gccaagctgc
     5101 cgattgccac gacgttcgtc agtcgtcata tcggcgccta aggagatccc atgaaagcca
     5161 gcgaactccg ctccaagaac gccaccgagc tcaaggaaga gctcgacagc ctgttgaggg
     5221 cgcacttcaa cctgcgtatg cagcgtgcga cccaacagct ttccaatacc agccagctgg
     5281 gcaaggtccg ccgcgacatc gcgcgcgtcc gcaccctgat gactgagacc gcgggaaagt
     5341 gaaatgactg aaacccaagt taccgcgccg gccaagcgtc agcgcacgct gatcggcaag
     5401 atcgtcagca acaagatgga caagacggtc gtggtgcgcg tcgaacgccg cgtcaagcac
     5461 cccgtctatg gcaagatcgt catgcgctcg aacaagtaca aggcgcacga cgagaccaat
     5521 cagtacaacg agggcgatac cgttgaaatc gccgaaggcc gccccatcag tcgcgacaag
     5581 tcgtggacgg tggtgcgcct gatcgaggcc gcgcgcgtca tctgatcgcc ggcggcttgc
     5641 cgtttgcgtg gctattggct gcgtgtggcg gtgcaagacc caggcaactc gattcgcagc
     5701 attccacccc ggatgccgga cgtcagtcca gcctccgggg tgttttattt ggcgcgtcgg
     5761 gccgctacgc agccttgtgg tcacgcagcc actcgcgcag cgccgcgttc atccgggttt
     5821 gccagccgtc gcctgtggca cgaaatgcat caagcacgtc ctgatcgtac ctgaccgtca
     5881 cggctatctt aggcgcggcc agtggtgggc gccccatgcg tcgggcgtga tgagcctctt
     5941 gttcgccaaa ctccatggcg tccgggtcgg cagcgatgcc ccggtcgatt cgaatatcct
     6001 cttcgggcgt gggaaggata gtcccgggtt taagtttcgg catatcgtct cacctctcgt
     6061 gagttggcct ttctcagact gatgatgcgt cgtacctcgt cacggtcgac gaaaaccacg
     6121 ctatacaggc gctgaccgat gtagcccagc gccacttggc gtggttcgcc atagtcgcgc
     6181 cggccatcat cccagcatag agcggtttcc cattccaacc gcacggccaa ggcgagcgag
     6241 acgccatgtt tgcgcaggtt gccatcgtct ttggccggat cgaacgagat ctgcatgtac
     6301 ttattgtagt aacgataagt gacggaggca aggccaagcc ttgcggtgat aacgcaaata
     6361 gacgctggcc gcgactgtaa gcctcattga gcatgatcgc gacttcacac cgctacccga
     6421 gatgttgcgc atcaatgcca gccggcccca tccaggttct gtcgtcaagg tgtacaaaat
     6481 caaggaaatc acccagaaat tcattgactg aggaatgcta tgagcgattt gctggaatac
     6541 aaaggctatc aaggttccgt ggggttcagt gcagaagatc attgtttctt cgggaaaatc
     6601 gaactcatca atgacttgat ctcattcgat ggaagcggcg tcgatgaaat cactgcggcg
     6661 tttcatcagg cggtggatga ttatctggcg ttctgtcaga aaacgagagc acaaccgaat
     6721 caaccattca agggaagctt caatgtcagg gtgacgccgg cgcttcactg gcgtaccgcc
     6781 gtcgaggtca agaaacgcgg aattaccttg gatcagttca tcgggcagag catcgagcgc
     6841 atgcttgatg gcgacatcga ggtgcatgat tgcatcaacc cagtgcgtac gaaggctccc
     6901 gcccacgcgt cttgatgcag gctattgagg gctgcgtcaa gacgcgtggg cctctggctt
     6961 gatgtgcgtc ggaaatggct ttacacgggg aattaccagg gaagggaata ggttttcacg
     7021 ttggtgtagc ccttcatggt ctcgatgacg ccttccttgt agcccaggcc cgaatccttg
     7081 atgccgccga acggggtgag ctccaggcga taaccgggga cctcgcagat gttgaccgtg
     7141 cccacctgca gttcgctgat gaagcgggtg atgtagtcca tgcggttggt gtagaccccg
     7201 gcggagaggc cgtaggggga ggcgttgacc aatccgatga cttcgtcgat ggtcttgaac
     7261 cgtgtgagcg gagacaccgg gccgaaggtt tcgtatttga tcaggtccat ctcgggccgc
     7321 actttgtcga ccagcgtggg gcaatactgg gcgcccacac gcttgttgcc atacaggagt
     7381 tcggcgccgt gctcgaccgc gttgatgacc aggctttcga atcgcatggc ggcttcttcg
     7441 tcgatcaccg tgccgatttc cgtggtgggg tccagcggat cgccgaactt gagcgccttg
     7501 gtttccttca tcagcagttc gccgaattcc ttggcgacgc tttcctgcac gaagatgcgc
     7561 ttgaccgcgg tgcaacgctg gccggagttc ttgtaggacc cggcggccgt catggcggcc
     7621 gccttggcaa ggtcggcgtc ctccatgatg atgaacgggg cgttgccacc cagttcgagc
     7681 acttggcgct tgtagaccgc cttcgcggcg atgtacttgc cgatggccac gccacccgtg
     7741 aaggtaacca ggtcgacgtc ggggttggtg agcatttcgt cggcgatatc tttcgggtcg
     7801 ccggtgatga ccgagagcat ttcgggcggc agcccggctt cgtagaggat gtcggccagc
     7861 aggatagccg cgagcggcgt tttttcggtg ggcttgagaa ccatgcggtt gttcgtcgcg
     7921 atggacgggg caaccttgtg gatgacctgg ttcagcgggt ggttgaaggg tgtgatggcc
     7981 gagatggcgc ccagcaacgg ctgccgcatg gtgtagacct tgcgctgttt gccgtgcggc
     8041 gtgaggtcgc aggagaacac ctggccgtcg tcgatgagcg cctggttggc cgagaacagc
     8101 agcacgtcgc ttgcgcggcc gacttcgtgc agcgtgtcca tcaggcacag gccggactcc
     8161 agggtgatga gccgcgcgat ttcgtcacgt cgcgccgcga tcaagtcgcg cgcacgcatc
     8221 aggatgttgt agcgatcggc ccgcgtgagc ttgctttgat attgcttggc gatgcggaag
     8281 gcccggcgca cgtcgtccag cgtggctttc gggacggtgg cgatgacttc gccggtgtag
     8341 ggataacaga cgtcgatggt ctcgtcgcgg tgaacccgtt caccggcgat gcgcatggcc
     8401 tcgacccgca cttcgtcgat cacgctgtcc tggctgacgc cgttcgtatt ggtgggaccc
     8461 acttcaagcc tctcttgatt gcaaatagtc aattggttgc atgccagagg ctgttggccc
     8521 gaagacacca cgctggggaa cccgcacatc ttggagcagc ttgcgcgggc gcggtaaagg
     8581 atgttgaacg ggcgtcaccg ccccatcatg tagaactcat cgttcggccg catgttcatc
     8641 acgttcgcca atctattcga cagtccgaag aacgccgcga tggcggcgat gtcccagatg
     8701 tcttcatcgt caaacccgtg gccatgcagc acagccatgt catcatcact cgaagcatag
     8761 gcgctggccg acaccttcat ggcgaagtcc agcatggcct tttgccttgg agtgatgtcg
     8821 gccttgcggt agttgacagc tacttgatcg gcgatgagcg ggttcttggc gcgtatgcga
     8881 agaatggcgc cgtgcgcgac cacgcagtat tggcattggt tggcgccact ggtggcgacg
     8941 acgatcattt cgcgctcggc cttgctcagg ttgccgggct tgtccatcag ggcgtcgtga
     9001 taggtgaaaa aggctcggaa ctcgtcgggg cggtgcgcca gaaccaggaa cacgttgggg
     9061 atgaagccgg atttctcctg cacggccagg atgcgctcac ggatatcgtc gggcaggtcg
     9121 gacacgttgg ggatggggaa gcggccgggg ggtggtgtgg tggtcatggg tagcgcttta
     9181 tgagatcgtg gaatgccgta gggccgttgt cgcaaggctg tttgggtggt tcgtgcaggg
     9241 ttggcctcgt caaaatggcg tgggtgaatc tgttgtggtg gtcacggcca cttcgtttcg
     9301 gcatctagtg ggtacacggg ccgttgcaca ttcttgaacg gaaacaggcc atagttggag
     9361 cttgtcaccc ccgcgctgtc gcattccaca aaccccctcg cgatggggaa gaacacgggc
     9421 cggcaataca tgcgcgattt cagcagcaag tagtctttct gcgtgggatc cagacccacg
     9481 ctcgtgaaca cgcccaggtc ccacggttcc tgcgtgcgtt cggtgacgac gatttccaga
     9541 tcgccacagg cgaacagagc ggtgtcaccc atgctgcact tgagaccagt gtaggtgggc
     9601 ccggtgatga tgtattcgcc attggtcagc ttcttgatca cgccggtgag ttccacgggt
     9661 ttcttcacga tgcccagctt ctccagcgcg cgcttgttgc ccagtttcac cgtgatggtg
     9721 gcaccttcgc cggcgtggcg catgagcgcc acggcttcgg ggtcgcacag cgggccaacc
     9781 ccgatgttgc ccaggccttg tgccagggct tcttgcagca catccatggt gtcgcaggtg
     9841 ccgccggaca tgcagttgtc gctgtgatcc agcagcagta cagggccttt ggggttggtg
     9901 cagccttcgg actggatgcc ggacagttcg gcgagttccg cggcgatgtt gatggattcg
     9961 gccagcggcg gactggtgta gacgaaaccc tcacggtcgg cccagatctg gcccgcgatg
    10021 cggcgcgcta cgcgctgcgc ggattcggcg tcgcggccca cgaccaccac gctgacacag
    10081 ggggcgggga tgtcggccag tgaaaacccc gccaggatgg acacggccag gacttcctcc
    10141 ttttccgccg cgcgcgcggc gtctatggcg gcgtgcatgg cgggcgacag gtcggcgctc
    10201 ttgagtgtat gggacatcag gggcggtcgt tcccacgcga tggccgcagt ggtcttgccg
    10261 gcaagcaggt ccaggaaaat ccgacccgcg tgttcgccgg tttcgtacat gtcgacgtgc
    10321 gggtaggtct tgaagctgat gatgacgtcg gcgttgtcga ccattttctg cgtgacgttg
    10381 ccatgcaggt ccagcgccac ggcaatgggc ttgtcggggt ggcgggtttt caggcgctcg
    10441 agcaagtcgc cttcgccgtc ctgggagttc tcggcgacca tggcgccgtg caggtccagc
    10501 atcagggcgt cgcagtcttg tgccgcgtcg atgatgcgct ggcacaaggt gttgtaggca
    10561 tcggcggcca cagggccgct gggattggcc gtggccgaca cgggtgtgac gatgtcggcg
    10621 ccggccgcct cggccagatc tatgtaggcg ctcatcgccg tgcgcccgcc atgattctcg
    10681 gcgtaggcgt cggcgccata ggtggggcca tcgatgccga aggccgacag cggcgtggga
    10741 accggcgaga acgtgttggt ttcgtggttc atgcgggcga tgaggaattt catggctacc
    10801 tgtgtcggga tggacgcccg ccgtgccaga cgcgacgggc gtgggtgggg cggggtcagc
    10861 cgcggggcac gatgccggcc tgcgcgagca tggcctgcag cagcacgttg gcgccggcgg
    10921 tgatgtgttc cggcttggca tcttcgatct cgttgtggct gatgccgtct ttgcacggga
    10981 tgaagatcat ggcggtgggg gcgacgcgcg ccacgtagac cgcgtcatgc ccggcaccgc
    11041 tgacgatgtc ggtatgcgaa aaacctagtt gctgcgcggc ggttcgcacc gcgtcgataa
    11101 gcgcgggcgc gaaaggttga ggcgggaaat acaccacctg ttcaagatgc acctccacgc
    11161 cttgtcgcga ggcgtcgtgg catgcttggg tgatggccgt gttcatggag tccagcacgt
    11221 cgtcggatgc cgcgcgcagg tccacgctga gccggaccgt gccggggatc acgttgcgcg
    11281 agttggggta gttgtcgatc cagcccaccg taccccgccc gtggggggcg tgatccagcg
    11341 cgatacggtt gaccgcctgg atcaggccgg cggcggcaag cagggcgtct ttgcgcagcg
    11401 ccatgggggt ggggccggcg tgggcctcca tgccggtgag ggtgacgtcg taccagcgct
    11461 gtcccagcgc accggtgacg ctgcctatga catcatcgcg ggcctcgagt atggggcctt
    11521 gctctatgtg cgcctcgaaa tacgccttga tggggaaagg gcggcaaggt gtggcgcccg
    11581 cgtagccgat ggcttcgagc gcttgcgcca cgctgacgcc ttcggtgtct tgctggccca
    11641 gggcgtgatc cagcgtgaag gcctcggcga agacgcccga ccccatcatg acgggaacaa
    11701 atcgcgaacc ttcctcgttg gtccagacca cgacttcgat gggcgcatcg gtctgtatgc
    11761 cctggtcgtt gagggtgcgc agcacttcca gccccgacag caccccgtag ttgccatcga
    11821 atttcccgcc cgtgggctgg gtatcgatat ggctgccggt gagcaccggc ggcaggctgg
    11881 ggtcgcgccc ggcgcggcgc gcgaagatgt tgccgatggc gtcgatgcgg acctcgcagc
    11941 cagcgtcctt ggcccagcct atgaaaagat cgcggccctg cttgtccagt tcggtgaggg
    12001 ccaggcggca caccccgccc ttgggtgtgg ccccgatgtc ggccaaggcc atgagcgacc
    12061 gccagaggcg ctggccgtct atgcgtgggg aggtttcaag tttgtctgga gcgttcatcg
    12121 tcgcctcggg tcgaaaattt cgggacgggc ggcccgttgt gcgagccacc agggtagggc
    12181 cggctcaagt gtcgtatcgt ggatgcgtcg atttcattat gcactcgaat tgatacgcca
    12241 gagggcacgg ggctagtcgg cgcccacgcc cagatactgg tcgtggacgt gggtgtctcg
    12301 tatgaacgcg gcgttgtcgc cggtgtagac gatctggccg ccttcaataa tgtaatgacg
    12361 ttcggccagt tgcgtgcaca cctgcaggtt ctgctcgacc agcaggatgg ctatgccttg
    12421 cgacttgatc tgatggagct gatcgacgat ctcttccacg atgaccggtg ccaggccttc
    12481 gacgggttcg tccagcatca gcagtcgtgg cgcgttcatg agcgcccggc cgatggcgag
    12541 catctgctgc tcgccgcccg ataactgagc gccgccgttg cgccggcgct cgcgcaggcg
    12601 cgggaagatg cggtagatgt cctgcaatga ccagggcgag tgcttcttgc gacccaggtt
    12661 gagattttcc tcgacggtga gcaagcggaa aatgccgcgg tgctccggca ccagacacag
    12721 gccttgcccc gcgatgcggt ggggggccag gcgcgtgatg tcgcgccctt cgaacagcac
    12781 ctgcccgttg gctggcgtga ccagcccggc gatgctcttg agcgttgtgg acttgcccgc
    12841 gccgttgcgg cccagcaggg tgacgagctc gccgtcccgc acttccaggc tcacgccctg
    12901 gatgacgtgg ctcttgtcgt agtaactgtg gatgtcgcgc acctgcagca tcagcggccc
    12961 ccggtgatca tgttgcccag ataggcgcgc ttgacacgct cgtcggcctt gatggcc
//
LOCUS       DASTNK010000032         7396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_206483, whole genome shotgun sequence.
ACCESSION   DASTNK010000032 DASTNK010000000
VERSION     DASTNK010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7396
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206483"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFR20_02535"
     CDS             complement(<1..188)
                     /locus_tag="VFR20_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229424.1"
                     /translation="MSSPDVILAEYIRRQTGADAVEVLEFARLAGGAIQDNHALTVML
                     TGGTHPGRLSVVVRSDAPS"
     gene            complement(194..1390)
                     /locus_tag="VFR20_02540"
     CDS             complement(194..1390)
                     /locus_tag="VFR20_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0229425.1"
                     /translation="MDFTLSDTVEDYRTRIRRFVDTELIPLEKDSASFDTHENIQEAL
                     LRSLRGKAREQGLWSLQMPRERGGQGLNMVGMAACYEEMNRSIFGPVVFNSAAPDDGN
                     MMVLEKVATPAQKKRWLQPIVDGDVRSSFAMTEPAPGSGSDPSMMRTCAVRQGDHWLV
                     HGTKHFITGAGVASHFILIARTSDDARKGLSAFLFHRDDPGWEIVRRIPIMGPEEHGG
                     HCELRFDGLRIPDENRLMEVGDGLKLTQIRLGPARLTHCMRWLGLAKRSLEVASDYVS
                     RRESFGKALAEREGVQWLLGEAAMGIQVGRLLTMHAAWKLDQGDFARKEVSMAKVAVS
                     ETLHKAVDTAIQLCGARGYSKDTPLEWIYRYARQARLVDGASEVHKMVLSRNLMAEGG
                     DFWHWG"
     gene            complement(1404..2171)
                     /locus_tag="VFR20_02545"
     CDS             complement(1404..2171)
                     /locus_tag="VFR20_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0229426.1"
                     /translation="MDDRFSLAGKTALVTGAAGGLGSRFAQTLGLAGARVVLAGRRLR
                     PLEELAESLGKLGVRATAVAMDVTDAASVHDALAQARAHVGLIDVAVCAAGIATNANA
                     LSLTPDQWDQVIDINLKGCWLVSSEIARRLIDAQCPGAIVHVSSILGHRVAGNVLPYT
                     VSKAGLEQMTRALALEWARHGIRVNALAPGYVETDLNREFFASDAGHAMLRRIPQRRL
                     GQAADLDGPLLLLASDASAYMTGSTIVVDGGHLQSTL"
     gene            complement(2269..3033)
                     /locus_tag="VFR20_02550"
     CDS             complement(2269..3033)
                     /locus_tag="VFR20_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU0229427.1"
                     /translation="MTDYMIEIDNLSKRFGDQIVLRDISLKVAKSSVVAIIGPSGSGK
                     STLLRCINLLTRPDGGQIRVGDQHIQFDGTDKCLPGERALAGFRANTGMVFQHFNLFP
                     HMTAVQNVMEGMVTVRKQSKPVAREAAMKLLARVGLAERADTYPDKLSGGQKQRVAIA
                     RALALQPQVMLFDEATSALDPELVGEVLAVMKQLADDGMTMVLVTHEIAFAREVADEV
                     FFMRDGVVVESGPPAQVINAPRQAATQAFLARFNAG"
     gene            complement(3072..3740)
                     /locus_tag="VFR20_02555"
     CDS             complement(3072..3740)
                     /locus_tag="VFR20_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU0229428.1"
                     /translation="MYHWNFAALWPYRDLLWHGLLYTLMFTVVCVVLGAAVGLASGLG
                     RLSRSVLISGPMRAYVEVFRCTPVLVQLIWFYYALPVLSGIQISPAMASILALSLYGG
                     AFYSEIFRAGVMSIDVGQREAGMAMGMTRHQIMRRVILPQALKRMIPPLMSQSIMQLK
                     NTSLLSVLAIPDLLYQGQTAAHDLYRPLECYSVVAICYFIVLFPATIFSKRLETRLAQ
                     QERG"
     gene            3971..5242
                     /locus_tag="VFR20_02560"
     CDS             3971..5242
                     /locus_tag="VFR20_02560"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEU0229429.1"
                     /translation="MHAVIVGGGIIGTTTAWQLRQRGADVTVIEQEPDVARATSRGNA
                     GVIAPGYVTPWAAPGMPFKILKYLFRSASPVIYRPAMNAAQWRWVARWLRECDLGKYR
                     VNKARMQRVAYYSKACLDEFRSAHPFDYGRSQGYLQLFRTAFDEDMARPAMQMLAEAG
                     INHRLLTADECLAVEPGLKWAGVRPASGLYLPEDEAGDCAVFAGRLRGLCERAGVRFM
                     FDTRVRGLLRSGTDVTGVAVSRGDGADKDNVQADAVIVAAGVHSRRLLAPLGITVPLY
                     PVKGYSTTLTVTDPGRAPRAALMDESLKTAITRMGPRIRVAGTAELGDDRLAIRQKAT
                     RTLIKVLNDWFPGAADTSRAEFWVGLRPMTPDGPPLLGKTSSRNLYINLGHGSTGWAM
                     SMGSACVVADLVVGKPPDIDLSGLTLERYRH"
     gene            complement(5391..6230)
                     /locus_tag="VFR20_02565"
     CDS             complement(5391..6230)
                     /locus_tag="VFR20_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0229430.1"
                     /translation="MPSRRQFVTGLGAAALGTALPLTRALAESTETAMARIRRTKTLR
                     SGVIAGAAPYFAKVVGTGKWEGFGPDFSAELAKSLGVEVKFVETTWGNAVMDLQANKI
                     DCMFGMGPTPQRRKMIGFTDPLFHNTFTLVARKGFDPKTWEEADKPDVKISVDIGSNQ
                     DAFATKTFHKAQIRRFDTSGDATLALQTGRVDAQLLVALLGVTVLAKAPGLGHIVVPT
                     PISGAPTCIGIQKETDTAFMTAVNQWLTKMHSSGETRRVILANMEKLVGIKAKDFPKE
                     VTL"
     gene            6479..7318
                     /locus_tag="VFR20_02570"
     CDS             6479..7318
                     /locus_tag="VFR20_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0229431.1"
                     /translation="MNTRQRFSALSVLAAACLTVPFSTAIAAGAPITAGSITNSPPMI
                     SYESDGTTLQGVIVDLAAAMGKEMGRPIVFKQIPFKGLLPAMQAKRIDITFTLMNDTP
                     AREKIIDFVDFFNLGTMLLIRKGNPDHVESLETMCGKTVSTVQGSTQIALVNEMSAKC
                     AAAGKPAIKNMEYAKPSDARLQVQTGRVAAFLGNTPVMVYLAKTAEGGKLFDVVRGHE
                     YQPVPLGIGVGKNDTALRDALQKSLNALIANGTYKKILEKYGVEGGAIKSATINGGKN
                     LKL"
BASE COUNT         1432 a         2332 c         2286 g         1346 t
ORIGIN      
        1 gagggcgcgt cgctgcgcac gacgacgctc agccgacccg ggtgtgtgcc accggtaagc
       61 atcaccgtca gcgcgtggtt gtcctggatg gcgccacccg ccaggcgtgc gaactccaga
      121 acttcgacag catcggcacc cgtctggcgc cggatatact cagccaggat gacgtcgggg
      181 gatgacatgc gccttacccc caatgccaga aatcaccgcc ctcggccatc aggttccggg
      241 acagcaccat tttatggacc tcggacgcgc cgtcgaccag gcgtgcctga cgggcgtaac
      301 ggtagatcca ttccagcggt gtgtctttgg agtagccccg cgcgccgcac aattggatgg
      361 cggtatccac ggccttgtgc aaggtttcgg acacagccac cttggccatg gagacctcct
      421 tgcgcgcgaa gtcaccctga tccagtttcc aggccgcgtg catcgtcaac aagcggccca
      481 cctggatgcc catggccgct tcgcccaaca accactgcac gccttcacgc tcggccagag
      541 ccttgccgaa cgattcacgc cgcgacacat agtcactcgc gacttccaga gaacgcttgg
      601 ccaggcccaa ccagcgcatg caatgggtca gtcgggccgg ccccagccgg atctgggtca
      661 gcttgagccc atcgccgacc tccatcagtc gattttcatc gggaatgcgc aagccatcga
      721 agcgcagttc acagtgccca ccgtgttctt ccgggcccat gatggggatg cggcgcacga
      781 tttcccagcc agggtcatcc ctgtggaaga gaaaagcgga caaccctttg cgggcatcat
      841 ccgatgtgcg ggcgatgagg atgaaatgcg acgccactcc ggcgccagtg atgaagtgct
      901 tggtgccatg gaccagccag tggtcgcctt ggcgcacggc gcatgtgcgc atcatggatg
      961 gatcggagcc tgagccgggc gccggctcgg tcatggcgaa tgacgatcgc acgtcgccgt
     1021 ccacgatggg ctgcaaccag cgcttcttct gcgctggtgt ggccactttt tccagcacca
     1081 tcatgttgcc gtcgtccggc gcggcggagt tgaacacgac cggcccgaaa atggagcgat
     1141 tcatttcctc gtagcaggcg gccatgccca ccatgttcag cccctgtcca cctcgttcac
     1201 gtggcatctg cagggaccac aacccctgtt cacgggcctt gcctcgcagg gaccgcaaca
     1261 acgcctcctg gatgttttcg tgggtgtcga atgaggcgga atctttttcc agaggaatga
     1321 gttctgtgtc cacgaaccgg cggatacggg tccgataatc ctcaacagtg tcggaaagcg
     1381 tgaaatccat gagaagcgag cctttaaagc gtggattgca gatggccacc gtcgaccacg
     1441 atggtgctgc ccgtcatgta ggccgaggcg tcagatgcca gcaagagcag tgggccgtcc
     1501 aggtcagcgg cttgacccag gcgccgctgc gggatgcggc gcagcatcgc gtggccggca
     1561 tcggaggcga agaattcgcg gttcaggtcg gtttcgacat agcccggtgc gagcgcattg
     1621 acccgtatgc catggcgagc ccattccagc gccagggcgc gcgtcatctg ctccaacccg
     1681 gccttggaca cggtataggg caagacattg cccgccacgc gatgacccag gatcgacgac
     1741 acatgaacga tggcgccagg acattgcgcg tcgatcaggc gccgcgcgat ttcactcgac
     1801 accagccagc aacccttcaa gttgatgtcg atgacctggt cccattgatc gggtgtcagg
     1861 gacagcgcgt tggcgttggt ggcgatgccc gcggcgcaaa ccgcgacgtc gatcagaccg
     1921 acgtgcgccc gagcttgggc cagcgcatca tgcacactgg ccgcgtcggt gacgtccatc
     1981 gcgacagcag tggcccgcac cccaagcttg cccagtgatt cggccagttc ttccagcggt
     2041 cgcaggcgcc gtccggcaag caccacccgc gcaccagcca gccccagggt ttgcgcgaaa
     2101 cgcgaaccca ggccaccggc cgcgccggtg accaaagcgg tcttgccggc caggctgaat
     2161 ctgtcgtcca tgcgccgtgt ctcctttttg gcgaaagtat agaacgatcg ttcgattttc
     2221 tcaaaaggaa atgaccagga ccacatccag ccaatttttc acctcggatc accccgcgtt
     2281 gaaccgcgcc agaaacgcct gtgtcgccgc ttgccgcggc gcgttgatga cttgcgccgg
     2341 cggcccgctt tccaccacca cgccgtcacg catgaagaag acctcgtcgg cgacttcccg
     2401 ggcgaacgcg atttcgtgtg tcaccagcac catggtcatg ccgtcgtcgg ccagttgttt
     2461 catgacggcc agcacctcgc ccaccagttc ggggtcgagc gccgaggtgg cctcgtcgaa
     2521 cagcatgacc tggggttgca gcgccagcgc gcgtgcgatg gccacgcgtt gcttctggcc
     2581 gccggagagc ttgtcggggt aggtatccgc gcgttcagcc aggccgaccc gggccagcag
     2641 tttcatcgcc gcttcgcgcg ccacgggctt ggactgtttg cgcacggtga ccatgccttc
     2701 catgacgttc tgcacggcgg tcatatgcgg aaacagattg aagtgctgga acaccatgcc
     2761 ggtgttggcc cggaaccccg ccagcgcgcg ctcgccaggc agacatttgt cggtgccgtc
     2821 gaactggata tgctgatcgc ccacgcggat ctgccctccg tcgggacgcg tcagcaagtt
     2881 aatgcagcgc aacaacgtgg atttgcctga accggacggc ccgatgatgg cgacgacgct
     2941 gcttttggcg actttcaggg aaatatcgcg gagcacgatc tggtcgccga agcgtttgct
     3001 gagattgtcg atctcgatca tgtagtcggt catggctcaa gcccgttcaa ctttgtgtgg
     3061 ttcatgccgc atcagcccct ttcctgctgg gccagacgcg tctcaagccg cttggagaag
     3121 atggtggcgg gaaagagcac gatgaaatag cagatggcca ccacggaata gcattccagt
     3181 gggcgataca aatcgtgggc ggcggtctgc ccttggtaga gcaggtcggg gatggccagc
     3241 accgacagca gcgacgtgtt cttcaactgc atgatggatt gactcatcag gggcggaatc
     3301 atgcgcttga gcgcctgcgg cagaatcacc cgccgcatga tctggtgccg cgtcatgccc
     3361 atggccatgc cggcttcgcg ctggccaaca tctatcgaca tcaccccggc gcggaagatt
     3421 tcagaataaa aggcgccacc gtacagcgac aacgccagaa tggacgccat ggcgggggat
     3481 atctgaatgc ccgacagcac gggcagggcg taataaaacc agatgagctg aaccagcacc
     3541 ggggtgcagc ggaacacttc cacatacgcg cgcatcgggc cggaaatgag cacactgcgc
     3601 gaaagccggc ccagaccgct ggccaacccc accgccgcgc ccagcaccac acagactacg
     3661 gtgaacatca gggtatacaa caacccgtgc cacagcaggt cgcgatacgg ccacagtgcc
     3721 gcgaaattcc aatggtacac agtgtctcct gacagtgaat tcgactcccg actcggtcca
     3781 cgaggcgatg aaaccggtat cggggaaacc ggcattttaa tgtgcaaccg gcgcctgccc
     3841 gcaccacggc gcccggtagc ggcgtttcga gagggtggct gggtcgttgg ggccagggcg
     3901 gagtcccgct gctgaccacg atgcaggaat ctataatatc gacaaaaaat tcaacgcata
     3961 cgggaatttc atgcacgcgg tcattgtagg tgggggaatc atcggcacca cgactgcctg
     4021 gcagcttcgc cagcgcggtg ccgacgtcac ggtcatcgaa caggaacccg acgtggcgcg
     4081 cgccacgagc cggggcaacg cgggcgtcat cgcgccgggg tatgtgaccc cttgggcggc
     4141 gcccggcatg ccgttcaaga tactgaaata tctgttccgt tccgcgtctc cagtgattta
     4201 ccgtcccgcg atgaatgcgg cccaatggcg atgggtggcg cgctggctgc gggaatgcga
     4261 tctggggaag taccgcgtga acaaggcacg catgcaacgc gtggcctatt acagcaaagc
     4321 gtgcctggac gaattccgct cggcgcatcc cttcgactac ggtcgcagcc agggctacct
     4381 gcagctcttc cgcacggctt tcgacgaaga catggcgcgg cccgccatgc agatgctggc
     4441 tgaagccggc atcaatcatc ggctgctcac cgccgacgaa tgcctggccg tcgaacccgg
     4501 tctgaaatgg gcgggcgtcc gcccggcgtc gggcctgtat ctgccggagg acgaagcggg
     4561 ggattgtgcc gtgttcgcag ggcggttgcg ggggctgtgc gaacgggccg gcgtacggtt
     4621 catgtttgat acgagggtgc gcggcctgct ccgttctggc actgacgtga caggcgtcgc
     4681 cgtcagtcgg ggtgatgggg cggataagga taatgttcag gccgatgccg tcatcgtagc
     4741 ggcgggggtt cacagtcggc gtttgctggc accgcttggc atcaccgtac ccttgtatcc
     4801 cgtcaaaggt tactccacga ccctgaccgt cacagacccc ggccgggctc cacgggccgc
     4861 actgatggat gaatcactga agaccgccat cacgcgcatg gggccgcgca tccgtgtcgc
     4921 cggaacggcc gagctcggtg acgaccgact ggccatacgc cagaaagcca cccgcacatt
     4981 gatcaaggtc ctgaatgact ggtttcccgg cgccgccgac accagccgcg ccgagttctg
     5041 ggtggggttg cgtcccatga cgcctgatgg gccgccgttg ttgggcaaga cctcgtcgcg
     5101 caatctgtat atcaatctcg ggcacgggtc cacgggctgg gccatgtcca tggggtcggc
     5161 gtgcgtggtg gccgacctgg ttgttggcaa accgcccgat atcgacctgt ctggcctgac
     5221 gctggaacgg tatcgccact gacacgttgc ggcgccgaat cgcttgctcc gaggccagtt
     5281 gtcgccaccg acactccagc gccatgggca ttgctgccat aaaagacaag ggcggccacg
     5341 cggccgcccg attgacgcaa aaccccggag ccttacagcc cacccggacc ttacagcgtg
     5401 acttccttgg ggaagtcctt ggccttgata ccgaccagct tttccatgtt ggccagaatg
     5461 acccggcggg tttcgccgct ggaatgcatt ttggtcaacc attggttcac cgccgtcatg
     5521 aacgcggtat cggtttcctt ctggatgccg atacaggtgg gcgcgcccga tatgggggtt
     5581 ggcacaacga tatggcccag tccgggcgcc ttggccagga ccgtgacgcc cagcagagcc
     5641 accagcaatt gcgcgtcgac gcggccggtt tgcagcgcaa gcgtggcatc acccgaggtg
     5701 tcgaagcggc ggatctgggc cttgtggaac gtcttggtgg cgaaggcgtc ctggttggag
     5761 ccgatgtcca ccgagatttt cacgtcaggc ttgtcggctt cttcccaggt cttgggatcg
     5821 aaccccttgc gtgccaccag cgtgaaggtg ttgtggaaca ggggatcggt gaacccgatc
     5881 atcttgcggc gctgcggcgt ggggcccatg ccgaacatgc agtcgatctt gttggcctga
     5941 aggtccatca ctgcattgcc ccaggtggtt tcgacgaact tcacctccac tccgagggat
     6001 ttggccagtt ccgcggaaaa gtccggcccg aacccttccc atttgcctgt gccgacaacc
     6061 ttggcgaaat agggcgcggc accggcgatg acgccggatc gaagtgtttt ggtgcggcgg
     6121 atgcgcgcca tggcggtttc agtgctttcc gccagagccc gggtcagggg cagtgcggtt
     6181 cccaatgcgg ccgcgcccag acccgtcacg aattgcctgc gtgatggcat gtctatctcc
     6241 tgtattgagt cattttgaat gttggcatgg gtgttgattg ccgttgcgcc gcctgccagt
     6301 gccggcggcg gacgctggcc aaacaccgca ccctcgcggg aaacgattat acgtgggcaa
     6361 acggtgggga atgtgtgccc gacaccgcta cgcgcgctgg atcgcctgtg ctacagtact
     6421 ttccgataac aagcccgaca acatcatgtc aaatgggccg ggcaccagga gaccatcaat
     6481 gaatacacgt caacgatttt cagctctttc cgtcctggca gccgcgtgct tgacagtgcc
     6541 attcagcacc gcgatagccg cgggggcacc catcaccgcc ggttccatca cgaacagccc
     6601 gcccatgatt tcctacgagt ccgacggcac cacgctgcaa ggtgtcatcg tcgacctggc
     6661 cgccgccatg ggcaaggaaa tgggccgccc catcgtcttc aagcagatcc cgttcaaagg
     6721 cctgctgccg gccatgcagg ccaagcgcat cgacatcacg ttcacgctca tgaacgatac
     6781 gcccgcgcgc gagaagatca tcgacttcgt cgatttcttc aacctgggca ccatgctgct
     6841 catccgaaag ggcaatcccg accacgtcga aagcctggaa accatgtgcg gcaagacggt
     6901 ttccaccgtg cagggctcca cgcagatcgc gctggtcaac gaaatgagcg ccaagtgcgc
     6961 ggcggccggc aagccggcca tcaaaaacat ggaatacgcc aaaccgtccg atgcccgcct
     7021 gcaggtgcaa accggccgtg tggcggcgtt cctgggcaac accccggtca tggtctatct
     7081 ggccaagaca gccgagggcg gcaaactgtt cgacgtggtg cgcgggcacg aataccagcc
     7141 agtgccgctg ggcataggcg tgggcaagaa cgacaccgcg ctgcgtgatg ccctgcagaa
     7201 gtcactcaac gcgctgatcg ccaacggcac atacaaaaag atcctggaga aatacggggt
     7261 cgagggcggg gcgatcaagt cggcgaccat caacggtggc aagaacctga agctctgatt
     7321 cacgtcctgc tgaagcactg atccatgcca tctggaactc tcgggcccac gcgggtcgtg
     7381 cccctgcgcc ggccgg
//
LOCUS       DASTNK010000033        22428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_208809, whole genome shotgun sequence.
ACCESSION   DASTNK010000033 DASTNK010000000
VERSION     DASTNK010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 22428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22428
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208809"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VFR20_02575"
     CDS             <1..393
                     /locus_tag="VFR20_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017685655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0229432.1"
                     /translation="LSAALAGLAGATKSVVFVSATLSDATWGMSGLVILMTLIGGLGT
                     FTGPVLGAIIIVMLENKVGEFGRFMAHATGLQWFQSLGESVTIVIGLIFVICVMAFRR
                     GIVGEILHAWHSRKPRNGHAPEAGAQPS"
     gene            complement(765..3029)
                     /locus_tag="VFR20_02580"
     CDS             complement(765..3029)
                     /locus_tag="VFR20_02580"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU0229433.1"
                     /translation="MAFSGLRGASSGLLAVLRKGSRLRKRRPGKAGSLPAPTDTASSI
                     ASLDTLTQVISGYLDPKDIKRVKEAYRFSDQAHLGQFRASGEPYISHPIAVTEICAGW
                     KLDAESLMAALLHDVMEDQGVAKQELAERFGTDVADIVDGLSKLDRLEFATKADQQAE
                     SFRKMLLAMARDVRVILIKLADRLHNMRTLGAVDPEKRHRVARETLDIYTPIAHRLGL
                     NALFRELQDLCFQALHPNRYRVLHKALLAARGNRREVLGKITEAVKVALPAAGIEAEV
                     TGREKSLFSIYNKMVEQKKSFSDVLDIYGFRVVVHTLPECYLALGTLHQLYRPVPGKF
                     KDYIAIPKLNGYQSLHTTLVGPYGTPVEFQFRTRDMDHVAEEGVASHWLYKDDAATLN
                     SLQKRTHQWLQSLLDIQSQTGDSGEFLEHVKVDLFPDAVYVFTPQGKILSLPRGATPV
                     DFAYAIHTDIGNQAVACKINSEFAPLRTELKSGDAVEIVTSPASRPSAQWLNYVRTGR
                     ARSEIRHYLRTVKYEESIAFGQRLLNQAFNQLHLPCPDDDDPEWEKLARSSGASSRDE
                     ILADIGLGKRLAAVVARRFAPENAMLATTAAAVDEITSTAGTPILIKGNEGQAVQLAP
                     CCGPLPGDAIIGGIRLGYGLVVHMEECAVAQRQRAREPERWVPVMWDTTTARHLSTRL
                     DVTVINERGVLGRVAAEITEADSNILQLTMQDDPSTAVLHLTVQVDSRKHLAQVIRAI
                     RHVSQVQKIVRIKG"
     gene            complement(3047..3250)
                     /gene="rpoZ"
                     /locus_tag="VFR20_02585"
     CDS             complement(3047..3250)
                     /gene="rpoZ"
                     /locus_tag="VFR20_02585"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU0229434.1"
                     /translation="MARITVEDCLEYIPNRFNLALVATYRARELAQGHEARLDTKDKP
                     TVTALREIAAGLTGMEMLRKVPT"
     gene            complement(3311..3943)
                     /gene="gmk"
                     /locus_tag="VFR20_02590"
     CDS             complement(3311..3943)
                     /gene="gmk"
                     /locus_tag="VFR20_02590"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU0229435.1"
                     /translation="MSHIGNTFMVVAPSGAGKSSLVNALLAQDPDVALSISCTTRPPR
                     PGEEAGKHYRFVDKDTFFAMRDQGNLLEWAEVHGNFYGTPRDRIDEAMRQGRDILLEI
                     DWQGARQVRKHFPEAIGIFILPPSIEALEARLYNRGQDAPQVISRRLLAAGSEMSHVS
                     EFEYVIINQEFSVALGQLAQIVSATRLRYATQAGRHAELFTQLGISATAS"
     gene            complement(4098..4415)
                     /locus_tag="VFR20_02595"
     CDS             complement(4098..4415)
                     /locus_tag="VFR20_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229436.1"
                     /translation="MIHARRPVGTLEQGLELDVQAEDFRFVAYLAVCEPDVDRVSDLV
                     PPERFAQEGDVHVAAIGTPEDATTQIEEVAFNMNPGDSVVFLCEDTDTYDAALAELDR
                     LPG"
     gene            complement(4412..4897)
                     /locus_tag="VFR20_02600"
     CDS             complement(4412..4897)
                     /locus_tag="VFR20_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0229437.1"
                     /translation="MAQQVPLLDDGLVIELGGGTGAVTQAILDQGIDPSRLMVVEYSR
                     SFVQRLRTRFAHINVIHGNAADLSQFVPPGQRVSAIVSSLPLCSLPAPVTQQILGQWQ
                     TLLADGGVAVQFTYHLRTPRWREHVAHVHARSKIIWANVPPAKVTTFSFDATSIAHPA
                     P"
     gene            complement(5181..5975)
                     /gene="xth"
                     /locus_tag="VFR20_02605"
     CDS             complement(5181..5975)
                     /gene="xth"
                     /locus_tag="VFR20_02605"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU0229438.1"
                     /translation="MKLATWNVNSLNVRLPHVLDWLRENPVDVLCLQELKQPDEKFPF
                     EAFQELGYHGARAGQKTYNGVAIISRAEGVDEQRNLPNFDDPQQRVLATTLPSPAGDV
                     RVICVYCPNGQSVGSDKYQYKLEWFQALAEWLKDQLVRYPRLAVLGDYNVAPADEDVY
                     DPEKWVGEVLVSEPEREAFRRLLGLGLADAFRLFPQEPAAFSWWDYRRFAFRRNAGVR
                     IDHVLLTPDLRVRCTACVIDKAPRAKDQPSDHTPVIATLDLDLPTK"
     gene            6379..7350
                     /locus_tag="VFR20_02610"
     CDS             6379..7350
                     /locus_tag="VFR20_02610"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEU0229439.1"
                     /translation="MKICIYGAGAIGGYMGAKLAQAGAEVSFIARGPHLAAMREHGVR
                     LREGDEEQAVKVRCTSDPAELGPQDYLIITLKAHSVPAVVDAMQPLLGPETAVVTGVN
                     GIPYWYFHEYGGELAGTVLESVDPGGRQWKGLGPRRAIGCVLYPAAEIIEPGVIKHVY
                     GKKFPIGEPSGELTPRIQALQRVMTDAGFDAPVREDIRDEIWLKLWGNLCFNPISALT
                     HATLDIIASDPGTRALARTMMLEAQAIAEKLGAHFRVGVDRRIDGAGAVGAHKTSMLQ
                     DLEHGRLMEIDPLLTVVKEMGTLTKMPTPTIDTVLALIKLRQRMAMA"
     gene            7634..7840
                     /locus_tag="VFR20_02615"
     CDS             7634..7840
                     /locus_tag="VFR20_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229440.1"
                     /translation="MGLLSLSMLAVFLLLAGAVMTGWIADHLPESVIACSDEGRFASF
                     AGEDHAVTASECGAVSATPHPSAI"
     gene            7851..8573
                     /locus_tag="VFR20_02620"
     CDS             7851..8573
                     /locus_tag="VFR20_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0229441.1"
                     /translation="MSAAEPHTEIRSNSLALSLQPINTGASLRDQAYVMLKQAIADAD
                     IYQSRELVRLDERVLSEALGVSRTPIREAMTLLEQEGFLRTVPRRGIYIQRKTKREIV
                     DMIQMWAALESMAARLATERASDDEIAALRTMFDDFRDSTPAEHIQEYSDANIAFHQA
                     IVELSKSQIILDTIKNLFVHVRAIRKLTISQSDRASRSIADHLRIIEALEKRDTDLAE
                     KLVRQHSLDLAAHVEAHCDFLD"
     gene            complement(8777..9658)
                     /gene="sucD"
                     /locus_tag="VFR20_02625"
     CDS             complement(8777..9658)
                     /gene="sucD"
                     /locus_tag="VFR20_02625"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010327286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEU0229442.1"
                     /translation="MAVLINNTTKVICQGYTGQQGTFHSEQAIAYGTRMVGGVTPGKG
                     GSDHLGLPVFDTVAEAVSATGADASVIYVPPPFAADAILEALDADVPLIVCITEGIPV
                     LDMLRVKRALAHSRSRLIGPNCPGIITPGECKIGIMPGHIHTAGKIGVVSRSGTLTYE
                     AVAQTTAAGLGQTTCIGIGGDPVNGTNFIDCLELFLADDDTEGIIMIGEIGGSAEEDA
                     ADFLRLSGTTKPVVGFIAGTTAPPGRRMGHAGAIISGGTGTAGAKIDAMRAAGIHVAE
                     SPSALGSTMLQAMAERA"
     gene            complement(9660..10868)
                     /gene="sucC"
                     /locus_tag="VFR20_02630"
     CDS             complement(9660..10868)
                     /gene="sucC"
                     /locus_tag="VFR20_02630"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEU0229443.1"
                     /translation="MNIHEYQAKALLGRYGVAVPKGLVAFSPEEARSNAEQLGGAVWV
                     VKSQIHAGGRGAGRFSNDPDGKGGVRVVKSVDDVAVNAARMLSSTLVTRQTGPAGREV
                     KRLYIEEGCDIARELYLGMLIDRATSRITIMASTEGGMEIEEVAHHSPEKILKVAVDP
                     ATGIQPFHARRIAYGLGLEGRQVKSATRFILSMYQAFTELDAQVVEINPLVVTADGDV
                     IALDAKMTFDDNALFRHPDVEALRDEAEEDPAERKAAEHSLNYVKLDGNIGCMVNGAG
                     LAMATMDIIKLYGGEPANFLDVGGGATRERVTAAFKLILADPKVQGILVNIFGGIMRC
                     DVIADGVVAAAREVDLDVPLVVRLEGTNVELGKQILEESGLPILSADNLADAADKVTR
                     AVAEATLGVN"
     gene            11220..12452
                     /gene="frc"
                     /locus_tag="VFR20_02635"
     CDS             11220..12452
                     /gene="frc"
                     /locus_tag="VFR20_02635"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HEU0229444.1"
                     /translation="MNPKALEGVRVLDMTHVQSGPTATQLLAWLGADVIKVELPGRGD
                     ITRGQLRDVPDADSLYFTMLNSNKRSITVNMKTDSGKAILEDLVRQCDVVAENFGPGV
                     LDRAGLTWERFQELNPRVIYASIKGFGPGAYVDCKAYENVAQCMGGASSTTGFEEGPP
                     VVTGAQIGDSGTGVHFALAITAALLQREKTNRGQRVQVAMRDAVLNLCRVKLRDQQRV
                     MRGPMREYPNAEFHDYVPRAGNASGGGQPGEALHCAPGGPNDYAYVIIQPQGWEPLMK
                     LCGREELITHPDYATPEARLKRLPECFAIVEEWTRQRTKFQVMEALNAVNVPCGPILS
                     TKDLIEDQGLYDSGYLVKLDHPTRGEYVQLGCPVTLSDSPVDVQRSPLLGEHTDEILS
                     WLGKSAAEIEKLHAEGAV"
     gene            12532..13308
                     /locus_tag="VFR20_02640"
     CDS             12532..13308
                     /locus_tag="VFR20_02640"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU0229445.1"
                     /translation="MTSWIRYVSPQRGPGFGLLDEEGCIHEHEGDMFDSPTPTGGRLS
                     LPDVTVLSPCQPGKIVALWNNFHALAAKIGKAVPSHPLFLIKPSTSVCGAGATIERPA
                     SYPGKIAYEGELGIVVGKTCRNVEPGAAANHIFGYTCVNDVTAIELLNEDANFAQWTR
                     AKSFDTFGVLGPVIVPGFDWRAANVVTTLDGVERQNYPLSDLVFRPEALVSLLSRDMT
                     LEPGDVIACGTSLGVGSMKSGASVTVTIKGIGSLTNVMEG"
     gene            complement(13502..13813)
                     /locus_tag="VFR20_02645"
     CDS             complement(13502..13813)
                     /locus_tag="VFR20_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0229446.1"
                     /translation="MQTTQLPLPVQARHPVVPRSDDDARLKAYNAVFSELGLRFRWNA
                     ATLDWMAGLDCDTEEARIAMYLETSQPHLLTAYDIDFLSHLIYDMKNKHSDNFGVLAA
                     A"
     gene            complement(13907..14857)
                     /locus_tag="VFR20_02650"
     CDS             complement(13907..14857)
                     /locus_tag="VFR20_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0229447.1"
                     /translation="MKNATLRQLKVFETVARQLSFSRAAEELHLTQPAVSTQVKQLEG
                     HAGLPLFEQLGKKIYLTPAGGEMLHYARSIIGQFREAEEAMVRFKGISGGKLNVAVIS
                     AGDYFFPRLLANFTRRHAGVILNLAVHNREELLHQLSANLTDLAVMVRPPLDMPTVNE
                     PIAPHPYVIVAAPGHPLAKKKNISLNRLAQEPFIVREKGSDTWNSMQEGFAGRLPNLK
                     IFMEISSTETIKQAVIAGMGISFLSIHTVSLELQVGNLVVLDVEGFPIMLNWYLVHRK
                     NKRLPPVALAFKNFLLEDGARLIAKYTHLPWETQSIHDHL"
     gene            15143..16483
                     /gene="oxlT"
                     /locus_tag="VFR20_02655"
     CDS             15143..16483
                     /gene="oxlT"
                     /locus_tag="VFR20_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HEU0229448.1"
                     /translation="MTQQEDAGLESGPFYTKRWVQLVIGIVCMALVANLQYGWTLFVN
                     PISTRHGWTRADIQVAFAIFILVETWFVPVEGWLVDRFGPRPVVAAGAILAALAWIVD
                     STAQSLPALYLGGLLAGLGAGCVYGTCVGNALKLFPDKRGLAAGLTAAGFGAGAALTV
                     IPIAHMIHTSGYEQTFMFFGIAQGVGIFILSMFLIRRKAPKGVRVSKMNMASQVNTPP
                     QKMVRTPVFWVMYAIFVAVATGGIMATAQIAPIARDFGLDNMPITLFGLTLPLLTMTL
                     SIDSLCNGFTRPLSGWISDLIGRENMMFIIFTCEGLAMLGLMHFGHHPAGFLIFAPLI
                     FLCWGEIFSIFPAISGDTFGTQYATVNNGLLYTAKGTSSLLVPVAALLAAGGRWDRVF
                     IAAATMAIVAAFCAKFVLFPMRKRWIESQTHAPSKVGATLPVGAHTVLSEQAGE"
     gene            16628..18076
                     /locus_tag="VFR20_02660"
     CDS             16628..18076
                     /locus_tag="VFR20_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0229449.1"
                     /translation="MNHPSTLQNHDLLINGRRVRPASGDYDLDLDPSDETPIAMIASG
                     NQADVDAGVAAARAALDGWANMRAADRGRILYRFADLLEQHQEEIVALESRDAGKPIS
                     AVRRQDFPAVIDTIRYYAGWADKINGSVVPVRPDALTYTRREPVGVVGVIIPWNFPLM
                     IGMWKIAPALACGCTLVVKPAEITPLSIMRAAELALEAGVPPGVLNIVTGKGSVVGEG
                     LVQHPGVDKVTFTGSPGVGRGIMRGAAGNFKRVTLELGGKSANLIFDDANVDAAVRAA
                     ASGIFFNTGQVCSAGSRILAQRKVYDEVVDKLVKRAKSMRVGAPSAADTNMGPLVSAR
                     QMDTVLKYVDIGKKEGATLATGGERLGDKGYYVSPTVFADVKHEMRVSQEEIFGPVAS
                     VIPFDDEADAIRIANGTVYSLAAGVWSADIARVHHVAHALKAGTVWINTYGYTDIRLP
                     WGGAGDSGLGREHGDTAIENFTEPKTVWLALG"
     gene            18266..19447
                     /gene="bktB"
                     /locus_tag="VFR20_02665"
     CDS             18266..19447
                     /gene="bktB"
                     /locus_tag="VFR20_02665"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketothiolase BktB"
                     /protein_id="HEU0229450.1"
                     /translation="MREVVVVGACRTAVGDFGGGLKEVPPCDLGALVVREVLSRSGVA
                     GEHVGHVVFGHVINTEPRDMYLSRVAALQGGVGQSTPAFNVNRLCGSGLQAIVSAAQS
                     LALGDAKVAIGAGAESMSRAPYIAPAQRWGARMGDSAMLDMMAGALSDPFDKVHMGVT
                     AENVASKYAISRADQDALALASHHRAEAAIQAGRFKEQIVPVVRKTRKGDVVFDTDEH
                     VRFGATADNFASLKPVFQKENGTVTAGNASGLNDGAAAVLLMTSEEARARGARPMARL
                     VAYAHAGVDPKYMGIGPVPATRLALEKAGLTIDRMDVVEANEAFAAQACAVSRELGLD
                     PEKVNPNGSGISLGHPIGATGAIITVKALYELERIQGRYALVTMCIGGGQGIAAIFER
                     A"
     gene            19440..20192
                     /locus_tag="VFR20_02670"
     CDS             19440..20192
                     /locus_tag="VFR20_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEU0229451.1"
                     /translation="MPSDVPTLSEQGGIRYLHFGTEWIQGAMDVRRPSELVLSYTRQM
                     MAWLLFLQPDRDSRMGILGLGAGSLLRFVLKHTPGSAVTVEWNPDVVAICRAYFRLPE
                     SIRSTVAQCDAADWLKRPGNLGRHLALMVDLYDSQAQGPVRESLEFYSDCRRALADTG
                     VLTVNLFGDHPSYARNLANIREAFNDRVLVLPEVDEGNVVVLALKGPLLEVSVRRFIE
                     RAAQVEAETGLPARRWARALLARRAGAPTFQA"
     gene            20222..21178
                     /locus_tag="VFR20_02675"
     CDS             20222..21178
                     /locus_tag="VFR20_02675"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0229452.1"
                     /translation="MRCIRLVVTAVIVALAVATGAARARTVDVVSGDGFPGQAAVEQG
                     IKDALANSGLTRLKHFNLNFRTLADGDAATQGVRDVAARKPDVIVAVGLAAARAAKAA
                     TATIPIVYVIVGDPVAAGLVDTQDASATNLTGVVDATDAQTRVTLIKQLIPGARRVGM
                     VYDPRHEDSAALVKHLRGMLPGDDMTLVDAVATRQADVGAAARSLVDKVDAIYTDNDA
                     IVESTYGMLVGLCDMMKIPLIASNVSDVKRGAVAAVGIDYHGLGMHAGKIAARILRGA
                     RPGNIPTVAVATPAIYVNLSAAAKQGVKLPDTVLASARVVVH"
     gene            21385..>22428
                     /locus_tag="VFR20_02680"
     CDS             21385..>22428
                     /locus_tag="VFR20_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0229453.1"
                     /translation="MSLKILSGVRVMRIAVVSSVFVFLTACGKAPPQGHGGMKVPVSV
                     VKVEPTKVTIYDNLPGRVEAIEDAQILARVTGIVTKINFEQGGLVKKGQLLFTIDPAP
                     YIAARDQAAAQLKQAQANVRSARSLAERYAKLVKVNAVSKQDYDNAVAQAAQSEAAVA
                     GAKATLETARINLGYTKVDSPITGRIGKALVTVGALVSASSATQLAAVQQIDRVYIDL
                     TRSTTEVLRMRRALASGRLKQVDAGAAKAIALLEDGSRYKHEGKLLFSGISVDPTTGQ
                     VNLRAEFPNPDGLLLPGMYVRVQLEQGVSNDALMVPQQAVQRTADGLSTLMLVKDGKV
                     SPQAVELGSQVNGK"
BASE COUNT         4069 a         6509 c         7281 g         4569 t
ORIGIN      
        1 ctgtcggccg cgctggcggg gctggcgggc gccaccaagt cggtggtctt cgtctcggcc
       61 acgctgtccg atgccacctg gggcatgtcg ggcctggtca tcctcatgac cctcatcggc
      121 gggctgggca cgttcaccgg cccggttctg ggggccatca tcatcgtcat gctcgagaac
      181 aaggtgggtg agttcggccg tttcatggcg cacgccacgg gcttgcagtg gttccaatca
      241 ttgggcgagt ccgtcaccat cgtcatcggc ctgatattcg tcatctgcgt catggccttc
      301 cgacgtggca tcgtcggcga gatcctgcac gcttggcact cccgaaagcc acggaatggc
      361 cacgcgcccg aggcgggggc gcagccttcc tgaggtggta tctgaaggcg ctgtctatcg
      421 gggtcaggag cagtccggca cggcgtgctt catccggatc tgtctcgtcc aggcgggcgt
      481 cgggcggaac tcacaaaaag gccagcccta tggactggcc tttttgttca gacatcgccc
      541 gacgactgcc tccgccttcc cgagccagac ccgggcagtc ggggcgcgca gcgccacgac
      601 cgctggacgc gccggcctgc accacctccc caccctgggg cgggttaata gacagcaccc
      661 caatccgaaa ccatccccgt cataaccgtc caaacggcaa cgcgatgcgt gttcaatgtt
      721 gcatgtgctt cgaccgaaaa agcccaggca tctgagcgtg gcactcaacc cttgatacga
      781 acgattttct gcacctggga aacgtgccgt atggcgcgta tcacctgtgc caggtgcttg
      841 cggctgtcga cttgaaccgt cagatgcagc acggccgtgg aagggtcatc ctgcatggtg
      901 agctgcagga tgttggaatc ggcctcggtg atttccgccg cgacgcggcc cagcacgccg
      961 cgttcgttga tgactgtcac gtcaagacgg gtcgacagat gccgggctgt ggtggtgtcc
     1021 cacatgaccg gcacccagcg ttcgggctcg cgcgcccgct ggcgttgcgc cactgcgcat
     1081 tcttccatgt gcacaaccag cccgtaacca aggcggatgc cgccgatgat ggcgtcgccg
     1141 ggcagcggcc cgcagcacgg cgccaattgc acggcttgac cttcgttgcc tttgatgagg
     1201 atgggtgtgc ccgcggtgga ggtgatttcg tcgactgccg cggcggtggt tgcgagcatg
     1261 gcgttctcgg gtgcgaaacg ccgggccact accgccgcca agcgtttgcc caggccgatg
     1321 tcggccagga tttcgtcgcg tgaactggcg ccggagctgc gcgccagctt ttcccattct
     1381 ggatcgtcgt cgtcagggca gggcaggtgc aactgattga atgcctggtt gagcaggcgt
     1441 tgcccgaagg cgatggattc ttcgtacttg accgtgcgca ggtaatgtcg gatttccgac
     1501 cgggcgcggc cggtacgcac atagttgagc cattgcgcgc tggggcgtga tgccggagac
     1561 gtgacgattt caacggcgtc gccgcttttc agctcggtgc gcagtggcgc gaattcgctg
     1621 ttgatcttgc aggcgacggc ctggttgccg atatcggtat ggatagcgta ggcgaagtcg
     1681 accggcgtcg cgccacgcgg cagggacagg atcttgccct gcggagtgaa gacgtagacc
     1741 gcgtcgggga aaagatcgac tttgacgtgt tcgaggaatt cgcctgaatc acccgtctgg
     1801 ctctggatgt cgagcagcga ttgcagccac tggtgcgtgc gtttctgtag actgttcagt
     1861 gtggcggcgt cgtctttgta gagccagtgg gatgccacgc cttcttccgc gacgtgatcc
     1921 atgtcgcggg tgcggaactg gaattccacg ggcgtgccgt atggccccac cagcgtggtg
     1981 tgcagcgact gatagccatt gagcttcgga atggcgatgt agtccttgaa cttgcccgga
     2041 accggccgat agagttgatg cagcgtgccc aacgcgagat agcattctgg caaggtgtgc
     2101 acgacgacgc gaaaaccgta gatgtcgagc acgtcggaaa acgacttctt ctgctcgacc
     2161 atcttgttgt agatgctgaa aagcgatttt tcacggccgg tgacttcggc ctcgatgccc
     2221 gccgcgggca acgccacctt gaccgcttca gtgatcttgc ccagcacttc gcgtcggttg
     2281 ccgcgcgcgg cgagcaacgc tttatgcagt acccgatatc ggttggggtg aagggcctga
     2341 aaacaaagat cctgcagttc ccggaacagc gcattgagcc caagacgatg cgcgatgggg
     2401 gtatagatgt ccagggtctc gcgcgcgacg cggtggcgtt tttccgggtc gacggcgccc
     2461 agtgtgcgca tgttgtgcag gcggtcggcc agcttgatga gaatgacgcg cacgtcgcgc
     2521 gccatggcca ggagcatctt gcggaagctt tcggcctgct gatcggcttt ggtcgcgaac
     2581 tcgagccgat ccaatttgga tagcccgtcg acaatgtcgg cgacgtcggt gccgaagcgt
     2641 tcggcgagct cctgtttggc gaccccctga tcttccatga cgtcgtgcag cagcgccgcc
     2701 atgagggatt cggcgtccag cttccagcca gcgcagatct cggtgacggc gatggggtgt
     2761 gagatataag gctcgccgct ggcgcggaac tggcccagat gggcctgatc ggaaaaccgg
     2821 taggcctctt tgacgcgctt gatgtccttg ggatcgaggt agccgctgat gacctgtgtg
     2881 agggtatcga gggacgcgat cgacgatgcg gtgtcggtgg gcgcgggaag gctgcccgcc
     2941 ttgcccgggc ggcgttttct gagacgggaa ccctttctaa gtacggccag taggccggat
     3001 gaggcgccgc gaagacccga aaacgccata tgcgtgctct agaggatcag gtgggcacct
     3061 tgcgcagcat ttccatgccg gtgaggccgg cggcgatttc gcgcagggcg gtgaccgtgg
     3121 gtttgtcttt ggtgtcgaga cgggcttcat ggccctgtgc cagttcgcgc gcgcgatacg
     3181 tggccaccag cgccagattg aagcgattgg ggatgtattc caggcagtct tcgacggtga
     3241 tgcgagccat agtgagtgat cctattagtg caagaggttg tgaggtgggg ccggacttgg
     3301 attgggcgtt tcaagatgcc gtggcgctga ttccgagctg ggtgaagagc tcggcgtggc
     3361 gtccagcctg tgtcgcgtag cgcaatctgg tggctgacac gatttgtgcc agttgcccta
     3421 gcgctacgct aaattcttga ttaataataa catattcaaa ttcagacacg tgcgacatct
     3481 cgctgccggc ggccagcagg cggcgcgaaa tgacttgcgg cgcgtcctgg ccacgattgt
     3541 acagccgggc ctccagcgcc tcgatggacg ggggcaggat gaaaatgcca atggcctcgg
     3601 ggaaatgctt gcggacctgg cgcgcgcctt gccagtcgat ttcgagcaga atatcgcggc
     3661 cttgacgcat ggcctcgtcg atgcgatcgc gcggcgtgcc gtagaagttg ccatggacct
     3721 ccgcccattc cagcaggttg ccctggtcgc gcatggcgaa gaacgtatcc ttgtccacga
     3781 agcggtagtg cttgccggct tcttcgccgg ggcgaggggg gcgcgtggtg caggaaatgg
     3841 acagtgcgac gtcagggtcc tgcgccagca gggcgttgac caggctggat tttcccgcgc
     3901 cactgggcgc cacgaccata aatgtattgc cgatatgcga cattggaatt gggctgatgg
     3961 gttcttgcaa aattattgaa cgcggaaaac ggaatcaatc acatcgttga gcggcttcat
     4021 cgaaaccgcc attttaacga atgcccgacc agtccgcgca ctgttgatgc tgactgtcgc
     4081 cgccgatgac gatgtcttca gcccggtagg cggtccaatt ccgccagcgc ggcgtcgtag
     4141 gtgtcggtat cctcgcagag gaacaccacg ctgtcgccgg ggttcatgtt gaaggcgact
     4201 tcctcgattt gcgtcgtcgc gtcttcaggg gtgccgatgg cggccacgtg cacgtcgcct
     4261 tcctgggcga agcgctcggg cggcaccagg tcggagacac ggtccacatc cggctcgcaa
     4321 acagccagat aagccacgaa acggaagtcc tccgcctgaa cgtctagctc caggccttgc
     4381 tccagggtgc ccacggggcg gcgtgcgtga atcatggtgc ggggtgggcg atggaggtgg
     4441 cgtcgaaaga aaaagtggtg actttcgctg gcggaacgtt ggcccatatg atcttcgaac
     4501 gggcgtggac gtgcgccacg tgttcgcgcc agcgtggcgt acgaagatgg taggtgaact
     4561 gcacggcgac accgccatcg gccagcagcg tttgccactg ccccagtatc tgttgcgtga
     4621 ccggcgcggg cagggagcac agcggcaggc tcgacacgat ggcgcttacg cgttgcccgg
     4681 gcggtacgaa ctggctcaag tccgccgcat tgccatggat cacgttgata tgggcgaagc
     4741 gagtgcgcag gcgctgcacg aaggagcgcg aatattcaac caccatcaac cgggatgggt
     4801 ctatgccttg atccagtatg gcctgcgtga cggcccctgt gccgccaccc aattctatga
     4861 cgagcccgtc gtcgagcaac ggaacctgtt gtgccatgta ggacgccagg aaacgggagc
     4921 ttgggcaaat ggcgccaatg gtggctgggc gggtgatgaa ttcccggatg aacgtccggg
     4981 gggcgatatt cctgacatac tctgtcagtc gttgggcgcg ttcaaaccgc acaactcgat
     5041 gcattattga gccttgtctt tttcaagttg cgtaacagta ccagcctggg gacgggaacg
     5101 tcataacgaa atgtaaatag gtgtggtcga aatggcaacc ctggcgacat gactcacgcc
     5161 caaatgggtg gcagcgctgt ctacttggtg ggtagatcga ggtccagcgt ggcgatcacc
     5221 ggcgtgtgat cggacggctg atccttggcc ctgggagcct tgtcaatgac gcacgctgtg
     5281 cagcgtaccc gcaggtcggg cgtcaggaga acatgatcga tgcgcacgcc ggcgttgcgt
     5341 cggaaagcga agcgccggta gtcccaccaa ctgaacgccg ccggttcttg cggaaacaga
     5401 cgaaacgcat cggccagccc caggccaagc aggcgccgaa aagcttcgcg ctcgggctcg
     5461 gacaccagca cttcgcccac ccatttttca ggatcgtata cgtcctcgtc ggcaggggcc
     5521 acgttgtagt cgcccaggac cgcgagccga gggtagcgca ccagttggtc tttcaaccat
     5581 tcggccagcg cctggaacca ttccagtttg tattgatatt tgtcgctgcc cacggactgg
     5641 ccgttggggc agtacacaca gattacacgg acgtcaccgg ctgggctggg tagcgtggtg
     5701 gcaagcaccc gctgttgtgg gtcatcgaaa ttgggcaggt tgcgctgttc gtccacgcct
     5761 tcggcgcgtg aaatgatggc gacgccgtta taggtctttt gacccgcgcg cgcgccgtgg
     5821 tagcccagtt cctggaaggc ctcgaacggg aatttctcat cgggttgctt gagttcctgc
     5881 aagcacaaca catcaacagg gttttcgcgc agccaatcca gcacgtgggg caggcggaca
     5941 ttgagtgaat tgacgttcca ggtggcgagt ttcatggatt tgcgggtgtc cttgtgtggc
     6001 gtgcctttgg tggtgatcgt tcggacggat attgcgtctg gccacccacc tggctcccat
     6061 ttgcaccgag gtgtcgtttt gaccctggaa agcgcacatt gtacgaggac tgaatcagtt
     6121 cgtgcgcggg cttaccagcg ctttgatgtt ttcgtcactg ataccgctgg agcgttcgcc
     6181 ggtcttcagc ggctggcggg cagtacgaac gtggcgctat cgtccgattc ggattgccgc
     6241 ggtttgccga ctcgttaaac tatttgttct gaatccgcgt cggccgtgcg caaatgcgct
     6301 tgcgtcttgg tcaattgaaa tataatatgt aatatatcaa tatagcaata atccgaattc
     6361 caatctcggg ggcgacaaat gaagatctgc atctatgggg caggggcgat cggcggctat
     6421 atgggagcga agctggccca ggctggagcc gaggtcagtt tcatcgcgcg cgggccgcat
     6481 cttgccgcca tgcgggaaca tggggtgcgt ctgcgcgagg gtgacgaaga gcaggccgtc
     6541 aaggtgcgtt gcacgtccga tccggctgaa ctgggtccgc aggattactt gatcattact
     6601 ctcaaagcgc attcggtgcc ggcggtggtt gatgccatgc aaccactgct tgggcccgag
     6661 acagcggtgg tgaccggggt gaatggcatt ccttattggt attttcatga atacggtggc
     6721 gaactggccg gtacggttct ggaaagcgtg gaccccggcg gccggcaatg gaagggcctg
     6781 gggccgcggc gcgccattgg ttgtgtgctg tatccggcgg ccgagatcat cgagcccggt
     6841 gtgatcaagc atgtctatgg caagaaattc cccatcggcg agcccagtgg cgaactcacg
     6901 ccccggattc aggcgctgca gcgcgtcatg actgacgccg ggttcgacgc ccccgtccgt
     6961 gaagacatcc gcgacgagat ctggctcaag ctgtggggaa atttgtgctt caaccccatc
     7021 agcgccctga cacacgccac gctggacatc atcgcgagcg acccgggcac acgcgccttg
     7081 gcccgcacca tgatgctgga agcccaggcc atcgccgaga aactgggtgc acatttccgc
     7141 gtcggggtgg atcgccgcat cgacggcgcg ggggcggtag gggcgcacaa gacgtctatg
     7201 ctgcaggatc tggagcatgg gcggctcatg gaaatcgacc cgcttctgac cgtggtgaag
     7261 gaaatgggaa cgttgaccaa gatgcccacg cccaccatcg acaccgtgtt ggcgcttatc
     7321 aaactgcgcc agcgcatggc gatggcatga cagcgtttct gtgatggtgg ccggcgaagc
     7381 cggccacgtg gctgaggtac ggggttctcg ccatggcggg gacacctttc acgctgtaca
     7441 ctgtatatca agggtttgtg cagtgcatgg cgccggttgt ggtaattctg tcacgggtat
     7501 tccagagaaa agtgttggtt tctggtatat cacatacaga atccttgact tgacgctgca
     7561 ctgcaacata caatggctcc atcagttgat tacttgatca ctgtctttca atcaatcact
     7621 tatggagttt gtcatgggcc tgttgtccct ttcgatgttg gcggtttttc tgctgttggc
     7681 tggtgccgtc atgactggct ggattgccga tcatttgcct gaatccgtga ttgcctgttc
     7741 cgatgaaggg cgtttcgcct cgtttgccgg cgaggatcac gccgtgaccg ccagtgaatg
     7801 cggcgctgtg tccgcaaccc cgcacccgag tgctatttga agagagcctg atgtccgctg
     7861 ctgaacccca taccgagatc cgttccaatt cacttgcgct gtcgctgcag cccatcaata
     7921 cgggtgccag cttgcgtgac caggcctacg tcatgctgaa gcaggcgatc gccgacgccg
     7981 acatctatca gtcgcgtgaa ttggtacggc tcgatgaaag ggtgctcagc gaggcactgg
     8041 gggtcagccg cacgccgatc cgcgaggcta tgacgctgct ggagcaggaa gggtttctgc
     8101 gcaccgtgcc gcgccgcggt atttacatcc agcgcaagac caagcgggaa atcgtggaca
     8161 tgatccagat gtgggccgcg ctggaaagca tggccgctcg tctggccacc gagcgggcca
     8221 gcgacgacga aatcgcggcg ctgcgcacca tgttcgatga tttccgcgat tcgacgccgg
     8281 ccgaacatat ccaggaatat tcggatgcca atatcgcctt tcatcaggcg atcgtcgaac
     8341 tctccaagtc gcagatcatc ctcgacacga tcaaaaacct gtttgtgcac gtgcgtgcga
     8401 ttcgcaagct caccatttca caaagcgatc gggcgtcacg ctcgatcgcc gatcatcttc
     8461 gcatcatcga ggcgttggaa aagcgcgata ccgatctcgc tgaaaagctg gtgcgccagc
     8521 attcgctgga tctggccgcg cacgtcgagg cgcattgcga tttcctggat tgatccgctc
     8581 gccagggtgg cgagtgatta ccgccagcaa tgtgaaacgc cggtcacttg accggcgttt
     8641 tgttttcatg gtgccaatga cgttctgccg attttccgct ctacttgttc cggggatccc
     8701 tgatcatgcg gggcgacggg ccttgtctgc gacacgccct tcgccgattc cgcccggcgc
     8761 aggttcccgc agcggtttac gcgcgctccg ccatggcctg cagcatggtg cttcccagtg
     8821 ccgagggtga ctcggcgacg tgaatacccg ccgcccgcat ggcgtcgatc ttggcgccgg
     8881 ctgtgccggt gccgcccgag atgatggcgc ccgcgtggcc catgcgccgc cccggtggcg
     8941 cggtggtccc ggcgatgaag ccgaccaccg gcttggtggt gcccgacagc cgcaggaaat
     9001 ccgccgcgtc ttcttccgcc gagccgccga tttcaccgat catgataatg ccctctgtgt
     9061 cgtcgtcggc caggaacagt tccaggcagt cgatgaaatt ggtgccgttg actggatccc
     9121 cacctatgcc gatgcaggtc gtttgcccga gcccggccgc tgtggtctgc gccacggcct
     9181 cgtaggtcag ggtaccggaa cgtgacacga caccgatttt gcccgccgtg tggatgtggc
     9241 cgggcatgat gccgatcttg cattccccgg gagtgatgat gccggggcag ttcggtccga
     9301 tcaagcggct gcgcgagtgc gccagcgcgc gcttgacgcg cagcatgtcc agcaccggaa
     9361 tgccctcggt gatgcagacg atcagcggca cgtcggcatc cagcgcctcc aggatggcgt
     9421 cggccgcgaa gggaggcggc acgtagatga ccgaggcatc ggcacctgtc gccgagacag
     9481 cttcggcgac cgtgtcgaac acaggcagcc ccaggtggtc ggatccgccc ttgccggggg
     9541 tcacaccccc caccatcctg gtgccgtagg cgatggcttg ttcagaatgg aacgtgcctt
     9601 gctggccggt atagccctgg cagatcactt tcgtcgtgtt gttgatcaga acggccatgt
     9661 cagttcactc ccagggtggc ttcggcaacc gcgcgggtca ccttgtcggc ggcgtcggcg
     9721 agattgtcgg ccgacaggat gggcaggccg gattcttcca gtatctgctt gcccagttca
     9781 acgttggtgc cttccaggcg caccaccagt ggcacatcga ggtcaacctc gcgtgccgcc
     9841 gcgaccacgc cgtcggcgat gacgtcgcag cgcatgatgc ccccgaaaat gttgacgagg
     9901 atgccttgca ccttgggatc cgccagaatg agcttgaacg cggcggtgac ccgttcgcgt
     9961 gtcgcaccac cgcccacgtc caggaagttg gccggctcgc cgccgtagag cttgatgatg
    10021 tccatggtgg ccatggccag acctgcgccg ttgaccatgc agccgatgtt gccatccagt
    10081 ttcacataat tgaggctgtg ttccgccgcc ttgcgttcgg ccgggtcttc ctcggcctcg
    10141 tcgcgcaggg cctccacgtc ggggtggcga aagagggcgt tgtcgtcgaa ggtcatcttg
    10201 gcgtccaaag cgatcacgtc gccgtcggcg gtgacgacca gcgggttgat ttcgacgacc
    10261 tgcgcgtcca gctcggtgaa tgcctggtac atgctcaata tgaagcgtgt cgccgatttc
    10321 acctgtctgc cttccaggcc caggccgtag gcgatgcggc gggcatggaa tggctggatg
    10381 cctgtggcgg ggtccaccgc caccttgagg atcttttcgg gcgaatggtg cgcgacttcc
    10441 tcgatttcca tgcccccttc ggtggaggcc atgatggtga tgcgcgatgt ggctcggtcg
    10501 atcagcatgc ccagatacag ttcgcgcgcg atgtcgcagc cttcctctat atagaggcgc
    10561 ttgacttcgc gcccggcagg gccggtttgc ctggtcacca gtgtcgacga cagcattcgc
    10621 gcggcattga ccgcgacatc gtccaccgat ttgacgacgc gcacgccgcc cttgccgtcc
    10681 ggatcgttgc tgaaccgccc cgcgccgcgg ccccctgcgt ggatttgtga tttcaccacc
    10741 cacacagcgc cgccgagctg ttcggcgttg gaccgggctt cttcagggga gaaggccacc
    10801 aaacccttcg gtacggcaac gccgtagcgc ccgagcaagg ccttggcctg gtattcatga
    10861 atgttcattt gtccctcatc gtcttgattg tctgggaaaa cccttgcttt cacgaaaatg
    10921 ccgcctgagg cgtcaccgaa cgatcatgat atgtgatata ttgaatttca acaagacaga
    10981 tagtcaaaca tccggcacga gtcccagtga gtgggcggga atcgcaagaa ataaacaccg
    11041 atgtagggca aatagggctt gtgtaaattt gatatatcac atattttatg tcagaagaga
    11101 tttcaatctt tgaaggagag agacgatcat gctcgcagcc accgacattc cacttcgcgc
    11161 ccatttcgag cgccacgccc ccgaccacca ttgattcaga ctcactcagg agacgataga
    11221 tgaacccgaa agctctcgaa ggcgtgcgcg tcctggacat gacccacgtg caatccggcc
    11281 ccacggccac gcagctcctt gcctggcttg gcgccgatgt catcaaggtg gaactgcccg
    11341 gccgcggcga catcacgcgc ggccagttgc gcgacgtgcc cgatgccgac agcctgtatt
    11401 tcacgatgct caattcgaac aagcgcagca tcacggtcaa catgaagacg gatagcggca
    11461 aagccatcct ggaagacctc gtgcgtcagt gcgacgtggt ggccgagaat ttcggccccg
    11521 gggtgttgga tcgcgccggc ctgacctggg agcggtttca ggagctgaat ccgcgcgtca
    11581 tttatgcgtc catcaagggt ttcggaccgg gtgcctatgt ggattgcaag gcctatgaga
    11641 acgtggcgca atgcatgggc ggcgcgtcga gcaccaccgg ctttgaagag gggcctccgg
    11701 tcgtcaccgg ggcgcagatc ggtgattccg gcacgggagt gcatttcgcg ctcgccatca
    11761 ccgcggcgtt gttgcagcgc gaaaaaacga atcgcggcca gcgcgtgcag gtggccatgc
    11821 gcgatgccgt gctgaacctg tgccgcgtca agctgcgtga ccagcagcgg gtcatgcgcg
    11881 gtccgatgcg ggagtatccc aacgccgagt tccacgacta tgtgccacgg gccggcaatg
    11941 cgtcgggtgg cggccaacct ggcgaggcgt tgcattgcgc gccgggaggc ccgaatgatt
    12001 acgcctatgt gatcatccag ccccagggtt gggagccctt gatgaaactg tgcggccggg
    12061 aagagttgat cacgcacccc gactatgcca cgcccgaggc gcgcctcaag cgtttaccgg
    12121 agtgtttcgc catcgtcgag gagtggacca ggcagcggac caagttccag gtcatggaag
    12181 cgctcaatgc ggtcaatgtg ccgtgcggtc ccatcctgtc caccaaggat ctgatcgaag
    12241 accaggggct gtatgacagc ggttatctgg tcaagctgga tcacccgacg cgcggcgagt
    12301 atgtgcagct cggctgtccg gtgacactgt cggattcgcc ggtcgatgtc caacgctctc
    12361 ccctgctggg cgagcacacc gacgagatcc tgtcctggct cggcaagagc gcagcagaga
    12421 tcgagaaact ccacgccgaa ggtgctgtgt gaggaatcgt ggcgcggggc cgcaccccgc
    12481 gccacgattt ccgcgccagg ggcgcgcacc catttccctg acggagaccg catgacttcc
    12541 tggatacgct atgtctcgcc gcaacgcggc cccggttttg gcctgctgga cgaggaaggc
    12601 tgcattcacg agcacgaggg cgacatgttc gattcaccca cgcccacagg gggccgcctg
    12661 tcattgccgg atgtcaccgt gctcagtccg tgtcagccgg gcaagatcgt tgccctatgg
    12721 aacaacttcc acgcactggc ggcgaagatc gggaaggctg tgccgtccca tcccttgttc
    12781 ctcatcaagc catccacatc agtgtgcggc gccggtgcga ccatcgagcg tccggccagt
    12841 taccccggca agatcgccta tgaaggggag ttgggcatcg tggtcgggaa gacttgtcgc
    12901 aacgtcgagc cgggcgcggc ggccaaccat atctttggct atacatgtgt gaacgacgtg
    12961 acggctatcg aactactgaa tgaagacgcc aatttcgcgc agtggacccg ggcgaagagt
    13021 tttgacacgt ttggcgtcct cggcccggtc atcgtgcccg gattcgactg gcgggcggcc
    13081 aacgttgtga cgaccctcga tggcgtggaa cgccagaatt atcccttgtc ggatctggtg
    13141 ttccggcccg aggcattggt gagcttgttg tcacgggaca tgacgctcga gcctggcgat
    13201 gtgatcgcct gtggcacctc gctgggggtg gggtcgatga aatcgggcgc ttctgtgaca
    13261 gtgacgatca aaggtattgg ctcgctcacc aacgtgatgg agggatgaac gaagaggatg
    13321 gatcaagggg cgggatgggg gcattacgac cgtcgatgtg ttgacgatcg aattctcgcg
    13381 ttggctgttt gaactgaacg ggacttcgtc ggccgcaagg caggcggccc cgttctggaa
    13441 aagaacgggg ccgtggcccg ccgtgaagtc agcgctttga gctgactctt gtgaggaaac
    13501 atcaggccgc ggccagcaca ccaaagttgt cgctgtgctt gtttttcatg tcgtaaatga
    13561 ggtggctgag gaagtcgatg tcgtaggcgg tgagcaggtg gggctgggac gtttccaggt
    13621 acatggcgat acgagcttct tcagtatcgc agtccaggcc ggccatccag tcgagggttg
    13681 ccgcgttcca gcggaaacgc aggcccagct cgctgaaaac cgcgttgtac gccttcagtc
    13741 gcgcgtcgtc gtcggagcgt ggaaccactg ggtggcgcgc ttggacaggc aagggaagtt
    13801 gcgtggtttg catgatcgat acctcctgtt ggcaatcatc ggttcctgat gacaccaagc
    13861 atagagaaca tcgagaatat atgataatta aagatttatt gaaaaatcat aaatgatcat
    13921 gaatggattg ggtttcccat ggcaggtggg tgtacttggc gatcaggcgg gcgccgtctt
    13981 caagcaggaa attcttgaag gccagcgcga ccggtggcag ccgtttgttc tttctatgca
    14041 ccaagtacca gttgagcatg atcgggaagc cctccacgtc gagcacgacc aggttgccga
    14101 cctgcagttc cagcgacacg gtgtgaatcg acaggaagct gattcccatg cccgcgatga
    14161 cggcctgttt gatggtttcc gtgctgctga tttccatgaa aatcttgagg ttcggcagcc
    14221 ttccggcgaa accttcctgc atggagttcc aggtgtcgga acctttttcc cgcacgatga
    14281 aaggctcctg cgccaggcgg ttcaacgaga tgttcttttt cttcgcgaga gggtggccgg
    14341 gcgccgcgac gatcacatag gggtgggggg cgatgggttc gttgactgtg ggcatatcca
    14401 ggggcgggcg caccatgacg gccaggtcgg tgaggttcgc cgacaactgg tgcagcaact
    14461 cctcacggtt gtgaacggcc aggttcagga tcacgccggc gtgtcggcgc gtgaagttcg
    14521 cgagcaggcg gggaaagaaa tagtcgccgg cgctgatcac cgcgacattc aatttaccgc
    14581 cggaaatgcc tttgaaccgg accatggctt cttccgcctc gcggaactgg ccgatgatgg
    14641 agcgggcgta atgcagcatt tcaccgccag cgggcgtgag gtagattttc ttgcccagtt
    14701 gctcgaacaa cggcagcccg gcgtggccct ccagttgctt gacctgggtg gagacagcgg
    14761 gctgggtcag gtgcagttct tcggcggcac gcgagaaact cagttgccgg gcgacggttt
    14821 caaagacttt aagctgtctc aaagtggcgt ttttcatggt gtaaacccgc agtacataag
    14881 tagctgtgaa tgattatata aagaaacttt aatttttatt tatttaactt tgattttagg
    14941 attaatcccg gtgagcgccg tctcaggccg cgttttccag gctgggcttc cccggaagcc
    15001 aatctcagat tcattacacg tttcagacag cgcggtgtgc tgttgcggtg gcggccaaag
    15061 gtcgttgtgg gtgcggtggt cacttgctcg tgtcgaagcc cgggaggttc gcgcgatgaa
    15121 catagaacgg agacaaatca tgatgactca gcaggaagac gccggacttg aatccggccc
    15181 gttttatacg aaaagatggg tgcagcttgt cataggcatc gtctgtatgg cgctggtggc
    15241 caatctgcaa tatggctgga cgctgttcgt gaatccgatc tcgaccaggc acggctggac
    15301 ccgcgccgac atccaggttg cgttcgcgat cttcattctg gtcgaaacct ggttcgtgcc
    15361 ggtggaaggc tggctggttg accggttcgg accccgccct gtggtggcgg cgggggcgat
    15421 actggcggcg ctggcctgga tcgtggattc cacggcgcaa tcactgccgg cgttgtatct
    15481 gggcggcttg ctggcgggcc tgggcgcggg ttgtgtgtat ggcacctgcg tgggcaatgc
    15541 cctgaagctg tttccggaca aacgtggtct ggcggctggc ctgacggccg cgggcttcgg
    15601 cgccggcgcg gcgctcacgg tcattcccat cgcccacatg attcatacgt ccggctatga
    15661 gcagaccttc atgttcttcg gcattgcgca aggtgtgggt atattcatcc tgtccatgtt
    15721 cctgatccgt cgcaaggcgc ccaagggtgt gagggtgtcg aagatgaaca tggcgtcgca
    15781 ggtcaacacc ccccctcaga agatggttcg aacccccgtg ttctgggtga tgtacgccat
    15841 tttcgttgcc gtggccaccg gtgggatcat ggccaccgcc cagatcgcgc ccatcgccag
    15901 ggatttcggc ctggacaaca tgcccatcac cctctttggc ctgaccctgc cgttgcttac
    15961 catgaccctg tcgatagaca gcttgtgcaa tggtttcact cgtccgcttt cgggctggat
    16021 ttccgatttg atcggacgtg agaacatgat gttcatcatt ttcacgtgcg aagggttggc
    16081 gatgctgggc ctgatgcatt tcgggcatca cccggccggg ttcctgatct tcgcgccgct
    16141 catcttcttg tgctggggcg agatcttctc catcttcccg gcaatctcgg gcgatacatt
    16201 cggtactcag tacgccacgg tcaacaatgg gcttctttac accgcgaagg gtacgtcttc
    16261 cctgctggtg cctgtggccg ccttgctggc cgcaggcggt cgctgggacc gcgtgttcat
    16321 cgcggcggcc accatggcca tcgtggccgc cttctgcgcc aagttcgtcc tgttcccgat
    16381 gcgcaagcgc tggatcgaaa gccagacgca tgccccgagc aaggtgggtg cgacgttgcc
    16441 ggttggcgcg cataccgtgc tgtccgagca ggccggcgaa taatcacctc gacagccccc
    16501 gcactgtgga tggcgcgggg gctgtcggca agagacacgg ggggatggcg cgttatcgac
    16561 agcagtacct gattgacatt cttatcatga atcaccggat atccatccag accaggagca
    16621 aaacagcatg aatcatccct caaccctgca gaatcatgat ctgctcatca atggccgtcg
    16681 cgtccgaccc gcctcgggcg actatgatct ggacctcgat ccgtccgatg aaacccctat
    16741 cgccatgatc gcctcgggca accaggccga cgtcgatgct ggtgtggccg ccgcgcgtgc
    16801 ggcgctcgat ggctgggcca acatgcgtgc ggccgatcga ggccgtatcc tgtatcgttt
    16861 cgcggatctt ctagagcagc atcaggaaga aatcgtggcg ctggagagcc gcgacgccgg
    16921 caagccgatc agcgctgtgc gccgccagga ttttcccgcc gtcatcgaca ccatccgcta
    16981 ctacgccggc tgggccgaca agatcaacgg gtcggtcgtg cccgtccgcc ccgatgcgct
    17041 cacttacacc cgccgcgagc cggtgggggt ggtgggcgtc atcattccat ggaattttcc
    17101 gctgatgatc ggcatgtgga agatcgcccc ggcactcgcg tgtggctgca cgctggtcgt
    17161 caaaccggcc gagatcacgc cgctgtccat catgcgcgcc gccgaactgg cgctcgaggc
    17221 cggtgtaccg ccaggcgtgc tgaatatcgt cacgggcaag ggcagcgtgg tgggcgaggg
    17281 attggtacaa catcctggcg tcgacaaggt cacgttcacc ggctcgccag gcgtggggcg
    17341 cggcatcatg cgcggcgcgg cgggcaattt caagcgggtg acgcttgaac tcggtggcaa
    17401 gtcggccaac ctgatcttcg acgatgccaa cgtcgacgcc gcagtgcgcg ccgccgcctc
    17461 gggcatcttt ttcaacaccg gacaggtctg ttcggcgggt tcacgcatcc tggcccagcg
    17521 caaggtgtac gacgaggtgg tcgacaagct cgtcaagcgc gccaagtcca tgcgagtggg
    17581 cgccccctcc gcggccgata ccaacatggg gcctctggtt tcggcaaggc agatggacac
    17641 cgtgctcaag tatgtcgata ttggcaagaa ggagggcgcg acacttgcca cgggggggga
    17701 gcggcttggc gacaaagggt attatgtttc ccccacggtg ttcgccgacg tcaagcacga
    17761 gatgcgggtt tcccaggagg aaatcttcgg gccggttgcc agcgtcattc ccttcgacga
    17821 tgaggcggat gccatccgca tcgccaatgg cacggtctac agcctcgctg ccggggtctg
    17881 gagtgccgac atcgcccgcg tgcaccacgt ggcccatgcc ttgaaggcgg gcaccgtatg
    17941 gatcaacacc tacggttata ccgatattcg cttgccctgg ggcggcgccg gtgactccgg
    18001 ccttggccgg gagcacggcg acacagccat cgagaacttc accgagccca agacggtgtg
    18061 gctggcgctc ggctagcgtt ttgacgctga tctggcgtcg gcgcaatagc cgaaaaaccg
    18121 cacatgagag catcatgtgc ggttttttca ttcctgaagg aataggttaa attaaggttg
    18181 cgccgcagca atgcgggacg tccatttttc cccgccttcc gcgtcagccg ggtggttgtc
    18241 gggagccacc gtttcggaga atgccatgcg ggaagtcgtt gtcgtgggtg cgtgtcgtac
    18301 agccgtcggg gattttggcg ggggcctgaa ggaggtcccg ccctgtgatc tgggggcgtt
    18361 ggtggtgcgc gaggtgttgt cccgctctgg ggtggcgggc gaacacgtcg gtcatgtcgt
    18421 attcggccac gtcatcaaca ccgaaccgcg tgatatgtac ctgtcccgcg tggccgcgct
    18481 gcaagggggc gttggccagt ccacccctgc gttcaacgtg aatcggttgt gcggctcggg
    18541 tctgcaggcc attgtgtcgg cggcgcagtc cctggctctt ggtgatgcga aggtcgccat
    18601 cggcgcgggg gccgagagca tgagtcgggc gccttacatc gccccggcgc aacgctgggg
    18661 ggccaggatg ggtgacagcg ccatgctcga catgatggcc ggcgccttgt ctgatccgtt
    18721 cgacaaagtg catatgggcg tgacagccga aaacgtcgca tcaaaatatg cgatttcgcg
    18781 ggccgatcag gatgccctcg ctctcgcttc ccatcatcgc gccgaggccg ccatccaggc
    18841 ggggcgtttc aaggaacaga tcgtgccggt ggtgcgcaag acgcgcaagg gcgatgtcgt
    18901 gttcgatacc gatgagcatg tgcgcttcgg cgccacggcc gacaatttcg cgtcgctcaa
    18961 accggtgttc cagaaggaaa acggcaccgt gacggcgggc aatgcctcgg ggttgaacga
    19021 cggggcggcg gcggtgctgc tcatgacgtc ggaagaggcc cgtgcccggg gcgcccggcc
    19081 catggcccgt ctggttgcct acgcgcatgc cggtgtggac cccaagtata tgggcatcgg
    19141 acccgtccct gcgacccgcc tggcactgga gaaggcgggg ctgactatag atcggatgga
    19201 cgtggtcgaa gccaacgaag cattcgcggc ccaggcttgc gcggtcagca gggagctggg
    19261 tctggatccg gaaaaggtca accccaacgg cagcggcatc agcctggggc atccaatagg
    19321 cgccacgggt gccatcatca ccgtcaaggc gctgtacgag ctcgaacgca tccagggccg
    19381 gtacgccctt gtcaccatgt gcataggcgg ggggcagggg attgccgcga tctttgagcg
    19441 tgcctagcga cgttccaacg ctgtccgagc agggcgggat ccgctacctg catttcggca
    19501 cggagtggat acaaggcgcg atggatgtcc gccggccatc ggagctggtg ctgtcctaca
    19561 cgcgccagat gatggcctgg ctgctgtttc tgcagcccga tcgtgacagc cgtatgggta
    19621 ttctgggcct gggcgccggt tcgctgctgc gtttcgtgct caagcatacc cccgggtccg
    19681 ccgtgacggt ggaatggaat cccgacgttg tggcgatctg tcgcgcgtac ttccgcctgc
    19741 ctgaatcgat caggtcgacc gtggcccagt gcgacgcggc cgactggttg aagcggccgg
    19801 gaaacctggg tcggcatctg gcgctgatgg tcgacttgta cgacagtcag gcgcaggggc
    19861 cggtgcgcga gtcgctcgag ttttattccg attgccgccg cgcgctcgcc gacaccggtg
    19921 tgctgaccgt gaacctgttc ggtgatcacc caagctatgc ccgcaatctc gcgaatatcc
    19981 gcgaagcgtt caatgaccgc gtgctcgtgc tgcccgaagt ggacgagggc aatgtcgtcg
    20041 tcctggcctt gaaggggcca ttgcttgagg tgtcggtgcg ccgcttcatc gagcgggccg
    20101 cccaggttga ggccgagacg ggcctgccgg cgcgtcggtg ggccagggcg ctgctggccc
    20161 gtcgtgcggg tgcccccaca ttccaggcct gatggcccta tccgtatcag gagttgtttc
    20221 catgaggtgt attcgactcg tcgtgaccgc cgtcatagtc gctcttgcgg tggcgacggg
    20281 ggctgcgcgc gcgcgcacgg tggacgtggt ttccggtgat gggttcccgg gccaggcggc
    20341 ggtcgagcag ggcatcaagg atgcgctggc caattcaggc ctgacgcggc tcaagcactt
    20401 caatctcaac ttccggactc tggcggacgg ggacgcggcc acccaaggcg tgcgggacgt
    20461 ggcggcgcgc aagcctgatg tcatcgtggc tgttggtctc gcggcggctc gcgcggcgaa
    20521 ggcggcgacc gcgaccatcc ccatcgtcta cgtgatagtg ggcgatccgg tcgcggcggg
    20581 gctcgtcgac acgcaggacg catcggccac gaacctgacc ggggtggtcg acgcgacgga
    20641 tgcgcagacg cgtgtcacgc tgatcaagca gctcattccg ggcgcgcggc gtgtcggcat
    20701 ggtgtacgac ccgcgccatg aggattcggc cgcgctggtc aagcacctgc gcggcatgct
    20761 cccgggtgat gacatgacgc ttgtcgacgc ggtggcgacg cgccaggcgg acgtgggcgc
    20821 cgcggcgcgc agcttggtcg acaaggtgga tgccatctat accgacaacg atgcgatcgt
    20881 ggaatcgacg tatggcatgt tggtgggatt gtgcgacatg atgaagatcc cactgatcgc
    20941 cagcaacgtg tcggatgtga aacgaggcgc cgtcgcggcg gtgggcatcg attatcacgg
    21001 gttggggatg cacgcgggga agatcgccgc gcggattcta agaggagcca ggccagggaa
    21061 cattcccacg gtcgccgtgg cgacgcctgc gatctatgtc aacctgagcg cagccgcgaa
    21121 acagggtgtg aaactgccgg acacggtcct ggcgtccgcc cgcgtggtgg tgcattgaat
    21181 caaccctggc ggctcgaccc cgcaagccgg taacgatctg aagtggtttg ttggcctggt
    21241 ttccccggtg tcaaacggta actgtccgat ttcgttttat actcgcttat ttcgcccaaa
    21301 tatcacccat cacgacggtt ccgcgcgcaa atgggcagca atattggaga gctttgcgcg
    21361 cgtacgatca gtgaggagtc ctgaatgtcc ctgaaaatac tgtccggcgt gcgcgtcatg
    21421 cgtattgccg ttgtgtcttc ggtttttgtg tttctgaccg cctgcggcaa agcgccgccg
    21481 caaggccatg gtggcatgaa ggtgccggtc agcgtggtca aggtggagcc caccaaggtg
    21541 accatttacg ataatctgcc tggtcgcgtc gaggccatcg aggatgcgca gatcctggcg
    21601 cgcgtcacgg gcatcgtcac gaaaatcaat ttcgaacagg ggggcctggt caagaaaggg
    21661 caactgctgt tcacgatcga cccggcgccg tacatcgccg cgcgggatca ggccgccgcc
    21721 cagctcaagc aggcacaggc gaacgtgcgc agcgcccgct cgctggccga gcgctatgcg
    21781 aagctggtca aggtgaacgc ggtcagcaag caggactatg acaatgccgt ggcccaggcc
    21841 gctcagtccg aggccgcggt ggcgggggcc aaggctacgc ttgaaacggc ccggatcaac
    21901 ctgggctaca ccaaagtgga ttcccctatc accgggcgca tcggcaaggc gctggtcact
    21961 gtcggcgcgc tggtcagcgc gagttccgcc acgcagctgg cggcggttca acaaatagac
    22021 cgtgtctaca ttgatcttac tcgttccacc accgaggtcc tgcgaatgcg cagagctcta
    22081 gccagtgggc gactcaagca ggtggacgct ggcgctgcca aggccatagc cttgctcgaa
    22141 gatggttcgc gctacaagca cgaggggaaa ctgctgtttt ccggcatcag cgtcgacccg
    22201 accacgggcc aggtcaattt gcgtgccgaa ttccccaacc ccgatggctt gctgttgccg
    22261 ggcatgtatg tgcgtgtcca gcttgagcag ggggtcagca atgacgcgct catggtgccg
    22321 cagcaggctg tgcagcgcac ggccgatggt ctcagcacgc tgatgctcgt gaaagatgga
    22381 aaggtctccc cgcaggcggt cgaactgggt tcgcaggtga acggaaaa
//
LOCUS       DASTNK010000034        37801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1320666, whole genome shotgun sequence.
ACCESSION   DASTNK010000034 DASTNK010000000
VERSION     DASTNK010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 37801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37801
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320666"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1549)
                     /locus_tag="VFR20_02685"
     CDS             complement(23..1549)
                     /locus_tag="VFR20_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEU0229454.1"
                     /translation="MSKSEKFLILNPADDVMIALRDLSKGDRVNNLELLNPVPLGHKI
                     ALRAVASGQPVRRYGQIIGFATRDIAAGQHVHMHNMEFREFDKDYRFSQDYVPTRVAD
                     KPDTFMGIVRPDGRVATRNYIGILTSVNCSATVAHAIADQFRRDIHPEALAAYPNVDG
                     VVALTHGVGCGIDASGEGLKVLQRTIAGYVRHPNFAGLLIIGLGCETNQISVMMKAHA
                     LREGSNLHTFNIEDVGGTPKSIAKGVELVKGMLAEANRAERTPVPVSHLKIGLQCGGS
                     DSFSGITANPVLGAAVDILVRNGGTAILSETPEIYGAEHLLTCRAATPAVGKKLVDIF
                     GWWEDVCHRNHASMNNNPSAGNKKGGLTTVLEKSLGGVAKGGTTGLMEVYDYAEPVQA
                     PGLVFMNTPGYDPMSATGQVAGGANIICFTTGRGSAFGCVPSPSLKLATNTVLWDRQR
                     DDMDINCGTVVDEGADIQQLGREFYEMIRRCASGERSKSEIYGYGQNEFVPWQPSVIC
                     "
     gene            complement(1559..2359)
                     /locus_tag="VFR20_02690"
     CDS             complement(1559..2359)
                     /locus_tag="VFR20_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0229455.1"
                     /translation="MQIFKTLPTLALCIAATACAFTAQANELTSIQKKGEIRIGVDIS
                     APPYGMVDKDAKKTGFDVDSARLLAKDLGVKLVIVPVTGPTRVQYLRTNKVDAIMASF
                     SITAERKKVIDFSEPYGVVPVVVFGPKAAKITGPKDLAKMSIAVTRGTTSDMTITDSV
                     KGVPGVRIVRYEDDATSNTAASTGQQKVLVGAPSVLPEILKANPKADLAYKYTATQFP
                     MGVGIRQNEPAFKARINKWVSTNLKNGQLNAIYKKYFFGQSLPENMLK"
     gene            complement(2416..3144)
                     /locus_tag="VFR20_02695"
     CDS             complement(2416..3144)
                     /locus_tag="VFR20_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU0229456.1"
                     /translation="MSPRVRFQNVRKSFGDLEVLKGISLEVPKGNVVALIGQSGSGKS
                     TALRCINGLEEIDGGELYVCDRALHAKDCDIPALRLQVGIVFQSYNLFPHLTVEKNIT
                     LAPRKVKNVGRKEAQELAAHVLGQVGLSDKIDAYPDQLSGGQQQRVAIARSLAMQPEL
                     MLFDEVTSALDPQITGEVLKVIEGLAKGGMTMVLVTHEMGFASAVADQIIYMREGVVW
                     ESGDRNLLKNPATPELRQFISNGL"
     gene            complement(3152..3808)
                     /locus_tag="VFR20_02700"
     CDS             complement(3152..3808)
                     /locus_tag="VFR20_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU0229457.1"
                     /translation="MILGVTYSQWLFILIGAGWTLVLSILGFVGGTVVGLPVAIARTS
                     HGWFFRILSAGYVKLIQGIPLPVVMFICYFGISIAGYDLPALVAAGIAMTLYSSAFLG
                     EIWRGCIEAIPRTQWEASESLALRKFQVLAHVILPQALRVAIPPTVGFLVQIVKNTSY
                     AVVIGFFDLTYSSRVINNSTFKPFVVFTVAALIYFAMCYPLSILSYRLERKLKRHSRS
                     "
     gene            complement(3805..4554)
                     /locus_tag="VFR20_02705"
     CDS             complement(3805..4554)
                     /locus_tag="VFR20_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU0229458.1"
                     /translation="MNYSFDFSFIGAYWPSLLHGLWVSTELSIISLIGGFVLGVALAV
                     ARSQGSPLARRLAAGFVDLTRNTPLIVQTFWLFFGLSALGFLVPAFYAAVVALSINTS
                     GYTCEIVRAGIDAVHYGQKEAAACLGLRRGQVMRHVVLPQAIEKMYPSLISQFVLMML
                     ASSIMSQISVEELTAIGLEIQSRTFRGFEAYVVIAVIYLVMSWLLRLVMAGCYNLAFP
                     KQRHLARARSSVGGRVKTGRVGGATASEVKS"
     gene            4972..5931
                     /locus_tag="VFR20_02710"
     CDS             4972..5931
                     /locus_tag="VFR20_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEU0229459.1"
                     /translation="MMGASEPRPPGVGEVAVRIRASSLNYHDLGVVSGRMPPSEQRIP
                     MSDGAGEIIAVGAGVEEFAIGDHVVSTFFPDWLDGNPRHAGFGRTPGDGIDGYAREQA
                     TMPATAFTHAPHGYSHAEAATLTTAGLTAWRALFENGQLKSGEAVLVQGTGGVSIFAL
                     QLAKRAGAQVIATSSSDEKLDKLRALGADAVINYRDDPEWGTTVRRLTDGRGVDHVVE
                     VGGPATLAQSITASRVGGHIAVIGVLSGRTSQLPLSQALSRQVRLQAILVGNRRQQQD
                     FIRAIEATGLRPVIDRHFPLDEIVAAFQHQASGRHFGKIVLDI"
     gene            6258..6719
                     /locus_tag="VFR20_02715"
     CDS             6258..6719
                     /locus_tag="VFR20_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU0229460.1"
                     /translation="MKHFTLKVNGREQKVEAEPDSPLLYVLRDDLNLKGPRFGCGLGQ
                     CGACTVIMDGNATRSCVFPVSAAVGKEIVTLEGLGSSQHPHKIQQAFIDAQAAQCGYC
                     INGMIMTAKAFLDKNPHPSAEDVRQALAYNLCRCGTHVRIVDAVMLAAKEA"
     gene            6721..8949
                     /locus_tag="VFR20_02720"
     CDS             6721..8949
                     /locus_tag="VFR20_02720"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0229461.1"
                     /translation="MDNHLNFDLTRRQLLKTGGWLAVGFSFAGPVAQAVAAAHGTTKP
                     KDFKKVDAWLTIDRNGHVTAYTGKVDLGTGVETALAQIVADELDVPFQSVTIVQGDTQ
                     LTPDQGPTWGSLTIQAGGAQMRKAAACARRAMLNLAARKLGVAADTLKVENGLIHSGG
                     KQLAYSDLAKGDTLDVAFDDKVPVKQPDAYSIVGKSIARVDIPGKVTGEFTFMQDFKL
                     PGMLHARVVRPTGMAANLESVDDSAARKVPGFVTTVVKGNFVGVVARTEWGAIRASQA
                     IKCKWSDWAGLPAMAQVYQDVKKTAVKQTVTPKNVGDAPGVIRSAAKSLKASYEYPVQ
                     THGSIGPSCSVADIGKDHARIWSASQSPHWLQGQIAKMLGFKPEQVHIIYIEGAGCYG
                     RNGHEDASADAALLSHALGKPVRVQWMRADEHGWDPKAPPYVADMEGALDEAGHVRAW
                     NFVTWLPARIGGLANVPLLADSLEHKDASVQTADNMNAGSMENDAEPPYSFPNVLATV
                     HRLETTPFRPAWLRGPGRLQNTYANECFMDELAAAAGTDPIAFRLSHLKDERGAACIQ
                     ACVKQANWVSRASPQKDTTGDVLKGHGFTYVFYDNSRTYVSAICEVEVNRATGKVRVT
                     RFVIAHDCGLIVNPDGVKAQIEGNVIQTTSRTLLEEVKWDSSHVTSLDWASYPILTFP
                     DVPKIEIVLINRPDKPAWGAGEPTCAVIAPAIANAIFDATGKHVRTVPFTPARVKAAL
                     TT"
     gene            complement(9008..10261)
                     /locus_tag="VFR20_02725"
     CDS             complement(9008..10261)
                     /locus_tag="VFR20_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU0229462.1"
                     /translation="MASKKTYFLGAVVVVVVAAGALLSGAFDPGYATSDVDKAPPSQQ
                     LIAKGRYLVRAADCAACHTTKDGAPFAGGVPLDTPFGRIYGSNITPDKEHGIGKWTSA
                     DFYRALHDGLAPGHPLYPAMPYTSYRGITRQDSDAMFAYLGSLRAVPQPDKKNTFTFP
                     YSARALMRGWNFLFLGDSLPDASKGASTQWVRGRYLANALGHCTECHTARGALGQLDL
                     RATLAGGDLGRIHAPDITPAGLAARGWSAQDLKQFLAHGVAPQGSVYSDMYEVFHLST
                     RHLTPADNAALVAYLTGDSPPAPAPLAKAAAGQTVEAGRLHYLALCAGCHAADGQGKP
                     NVSVAMRANSTLRDADPHNLVAVMLDGIGAQTFTGNAAMQAMPGFAPRLDDQAMADLA
                     NYLRVTWGGHKGDVTPAMVAGMRGK"
     gene            complement(10251..10811)
                     /locus_tag="VFR20_02730"
     CDS             complement(10251..10811)
                     /locus_tag="VFR20_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU0229463.1"
                     /translation="MSIATKSLSMIINGKPVGPFQVPESLMMIDFLHEYAGLTGSRLG
                     CGQGICHACVAILDHEDGTSEEIRTCITGALYFQGRKLRTIEGHAQRNQEGEVTALSP
                     VQQKFLDFYSFQCGYCTPGFVNAATVLLEKLKRHPIEKSQVEAVVTDALNDHICRCTG
                     YVRYHNAVKDLILATPDLLKESAHGK"
     gene            complement(10808..13633)
                     /locus_tag="VFR20_02735"
     CDS             complement(10808..13633)
                     /locus_tag="VFR20_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0229464.1"
                     /translation="MAKPDLPRRRFVKTMVVAGITAYIAPFGSTAYAALFDDERLTPV
                     RWDAAAGKMEFRVDGVAKVTGSKIFARDIRARDLPHWPDRQSHAFMLRATRADALYEG
                     FDLSRLGADLKPDRVVTAEDLARDKVAFTDFYGEDMLLPKGKTPAYLGHAVAILIFHD
                     FARFRLAKSALQFQADVIKYGARTGPLQRDPWGTFRFVRVGGQTPYDDDVFSSLKDAP
                     IFPSQMRKHLPIWPDGKDHGSLGEEGMYYAGQIRGEFDNPRQDELVLRRTYHTQSVDT
                     SALEPDNANCWYDAANQVMHMVVPTQSPTEVASTASEMLAKTAFPIKSVVLHPCYTVG
                     YGSKDHCNMPFYGLACALYADSRPVRLANDRYEHFQSSLKRHAFDIDYQVIVDRKTGL
                     FRTFVADMVANGGGRCNFSPSVAMVGATAAQTIYYLPKSDLTSRAEATRAVDAGSARG
                     YGTLQSMAATEMLVDEIAGQLKLDPIEFRLRNVLKTGMKNTQGAVPAGQVRADEVLLK
                     AREHPLWRDREKRKKAYEASHPGKLYGVGFACVQKDFGTGAESSSARVEVSPDGRVLL
                     RHTGTEIGTGMSTSQAIACVKWLGSPATDLGYAITDWPQLPMKTSGDPYLSSQADQDR
                     AAADPMWTPAIASPASASNSAFYFTHTTREACRLIFRHGLWPAALAIWSQGLGGGQAA
                     PYTVREEDARWEDGLLTANGMQGLGLAQLAQKAHDMGLVVGAVVHGFNRWQWAEADFP
                     IGGKTERLPLDGLALLYGKGGGAAASGGKAGPGYQIVRRQTAYYPPVQRNNAGVTYYS
                     AMGTLAELTVDRGNGKVELLNHHSIIECGNMLSPELVSGQIQGGIAMGIGHALYEHLP
                     LYEDGPGNGTWNFNRYHLPLAKEVALWSQTAEVLPPLSDTDPPKGMGEVVMIPIVGAI
                     VNGLAHAMGHRFMSLPVTAEKIQEVLA"
     gene            complement(14098..14529)
                     /locus_tag="VFR20_02740"
     CDS             complement(14098..14529)
                     /locus_tag="VFR20_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229465.1"
                     /translation="MTKFPLSGALGAVLCVTLAACSTTGNPDKMNQLSQKTVQYQCSA
                     QGQRTVNLEVQYTFQGNDPVTAQVIYDNQAIALTRATNSKTDMVGDTFTGNGYTWTTG
                     KFTLESAGQVNGNMLIRQETSAGTTGAPTNTILARNCKAGG"
     gene            complement(15122..15198)
                     /locus_tag="VFR20_02745"
     tRNA            complement(15122..15198)
                     /locus_tag="VFR20_02745"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15162..15164),aa:Pro,seq:cgg)
     gene            complement(15209..16174)
                     /locus_tag="VFR20_02750"
     CDS             complement(15209..16174)
                     /locus_tag="VFR20_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU0229466.1"
                     /translation="MNLAELTHPIADDMKAVDAVIRARLNSDVVLIRTVGDYIVGAGG
                     KRMRPALLLLLAGALGYRGTHHHLLAAVVEFIHTATLLHDDVVDESDLRRGRDTANAV
                     FGNAASVLVGDYLYSRSFEMMVEADSMPAMSVLSGATTVIAEGEVLQLLNVHDPDVSL
                     DRYLQVVRYKTAKLFEAATQVGAIVAGAPAGLIEAAARYGRHVGTAFQLIDDVLDYSG
                     DVHALGKNVGDDLREGKPTLPLIRVMEVGTPAQRDLVRQAIVSGEGDFTAVARAIHDT
                     DALDYTRRAAEAEADIARDALAPYPVSVYKDSLLKFCSFAVERDR"
     gene            16427..17293
                     /locus_tag="VFR20_02755"
     CDS             16427..17293
                     /locus_tag="VFR20_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0229467.1"
                     /translation="MSTTDLTTLIDRAARVLAQLEAWLPPAPPPIDWSAHAFQWRKRG
                     TRGWLDAVQNVSLIHIADLQHVERQKTIIDRNTRQFLTGLPANNVLMTGARGTGKSSL
                     VKAMLAAYGDRGLRLIEVDKSDLADLADIVDLVSSRPERFIVFCDDLSFEEGEAGYKA
                     LKSVLDGSLSAAGENVLIYATSNRRHLMPEYMSENLSAKHQPDGEIHPGETVEEKISL
                     SERFGLWLSFYPFKQDDYLDIVNYWLGQHGCSADTIAASRTEALQWALERGSRSGRVA
                     RQFARDWAARHV"
     gene            17286..18359
                     /locus_tag="VFR20_02760"
     CDS             17286..18359
                     /locus_tag="VFR20_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU0229468.1"
                     /translation="MSKPLIEVAAGLLLRSDGQLLLAQRPGDKPWPGWWELPGGKIEP
                     GESTLDALARELREELGIEVTAATPWVTYTHEYPKNHVRLAFCRVTGWRGDPRGVEGQ
                     TLAWVDPHASIPIAPLLPATEPPLRWLRLPNRYLVSSIGNDAGLPAFLEKLEHALEGG
                     LKLVQFREPGWDAAPAHSGLRRVLAACRAYGARCLVNSCHPEVWWAEADGVHWRATDA
                     RRQAAIIAAGAASASASASASNTSDTSAPHQATAAEAGQTALRFFPATDPTRRLVAVS
                     AHDADDLAAARALGADFAVLGHVLDTPSHPGVPGMGWTRFAQLRENAGLPVFAIGGQS
                     ADTVADATRHGAHGIAGIRYLGS"
     gene            complement(18344..19867)
                     /locus_tag="VFR20_02765"
     CDS             complement(18344..19867)
                     /locus_tag="VFR20_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEU0229469.1"
                     /translation="MAVLLAGCAVGPDYHRPAVDMGQQYKEVKGWVQAKPAGVSLHDD
                     WWRLYRDPVLDGLMHTLQDANLSVAQSEAQYREARAALESTRAGLFPTVGLSSSAARA
                     GGGTGQSGSSNFSGGGTSSSYVLSGTVTWEADLWGKVRRSVEAGQAELQASAADVAAT
                     RLSMESTLAQDYFQLRALDAEKRMYQQTVAAYERSLKVTVNRYNAGVSAKADVASAQT
                     QLENARAQMQALEWQRAQYEHAIAVLIGKPPSRFSLAEAQSIGIVPIIPVGLPSQLLQ
                     RRPDVAAAERRTAEANAKIGVAQAAWFPDLTLSAQGGFRSGEWVNWLTAPMRFWSLGP
                     ELAMTLFDAGARSAQVEQAQAAYDAQVASYRLTALTALREAEDYMVQLRVLARQQVTQ
                     NRALAAARESLRLTTNQYDAGLIDYLSVAVVQASTLSTERAALSVTVDRLVASVQLIA
                     ALGGGWAPAPAMDTATIGAKAAPGSIGTASTDVKEGTTSAGAGNGASVTQGSAQEPR"
     gene            complement(19986..23087)
                     /locus_tag="VFR20_02770"
     CDS             complement(19986..23087)
                     /locus_tag="VFR20_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEU0229470.1"
                     /translation="MRWLSLFVMRPVATTLLALTLVLTGALAFRFLPVSPLPEVDFPI
                     ISVSANLPGASPETMASSVATPLEQSLGTIAGVTDMSSVSTEGSTRITLQFDLSRNIN
                     SAARDVQAAINAARSLLPSSLRTPPTYHKVNPAAAPIMVLALTSNTVSQGSLYDLAST
                     VLAQKLSQVSGVGEVSVGGSSLPAVRVNLNPQALVNAGVALDDVRTALANANTMRPDG
                     MVENDQYHWVLSSGSQLTKAAQYRPLIVAWRNGAPIRLGDVATVQDSVEDLYQTGFFN
                     DKKAILLIVRRQSDANIIETVDGIRAELPSLEALMPADVSLTVAQDRTPSIRASLHEA
                     ELTLVIAVLLVVLVVLMFLRNARAALIPSVAVPVSLIATFSLMQLFGFTLNTISLMAL
                     IVAAGFVVDDAIVVLENIIRHMERGVPPLRAALRGLREVGFTVVAMSLSLVAVFIPIL
                     LMGGIVGRLFREFAVTLSAAIMVSLVVSLTLTPMMCAHVLKHTPAADAETRPGRVARL
                     IQAIGAWLWNGYKRSLGWALAHSRFMMLLLAATIGLNFYLYTVVPKGFFPQQDTGQLL
                     GFFRADQGTSFTAMIPKLDDFEKTIRQDPAVQTVTGFAGGRGGSNSSFLLIQLKPFSE
                     RTASADQVVNRLRKKFLDVPGARLTLVPQQDIRVGRHESSTAAYNYTLMASDLALLRQ
                     WMPQVQKALAALPELADVDTDVQDKGRQIELVVDRNAASRLGVNMSLISSTLNDSFSQ
                     RQVSVIYGALNQYHVVMGVDPSQAQDARALEQVQVVTAAGNRIPLSAFTHFVVENAPL
                     SVQHQGLFASDSVSFSLAPGFTLQQATQAIDQAVARIGLPTREIQAGFLGEAGQLQQV
                     LSQQPWLILAALVTMYIVLGVLYESYVHPITILSTLPSAGIGALLALILLGQEFTLIA
                     LIGVFLLIGIVKKNAIMMVDFALLAEREQHLSSRDAIYEACLVRFRPIMMTTVSALFG
                     ALPLVLASGAGVELRRPLGLTIVGGLILSQILTLYTTPVVYLYLDRFRWWANRKRGIT
                     KHG"
     gene            complement(23084..26191)
                     /locus_tag="VFR20_02775"
     CDS             complement(23084..26191)
                     /locus_tag="VFR20_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEU0229471.1"
                     /translation="MNFSRLFILRPVATTLLMIALLISGVLAYRLLPVSALPEVDYPT
                     IQVVTLYPGASAEVMTALITSPLERQFGQMPGLSQMTSTSSGGSSQITLQFDLTLSMD
                     VAEQEVQAAINAASNLLPTDLPMPPTYNKVNPADTPVISLAITSPTLPLYEVRDLIDV
                     RMAQKLSQIPGVGLVSIAGGQRPAVRIEVNPQALAQHNLTLEDVRTAVTGANVNQPMG
                     NLDGPQRSITINANDQLKSVSDYENLILKYENGAALHLGNVATVRQNPEDIRQAAWIG
                     DTPAVLMNIQRQPGANVIDVVDRVKALLPSLKKALPAGVDVAVATDRTYTIRDSISHV
                     EREMLLAIFLVVLVTFVFLRTWSATLVPSVVVPLSLVGTFGIMYLAGFSINNLSLMAL
                     TVATGFVVDDAIVMIENIARHIEEGETAMRAALKGAAQIGFTLVSLTVSLIAVLIPLL
                     FMSDVIGRLFREFAITLSVAILMSLLISLTLTPMMCARLLKAESEQKYGRVQVALGRG
                     MDTLVGWYDHGLKWVLSHQRITLWIALGTFVVTGLLYGLVPKGFFPQQDTGLIQAISQ
                     APATVSFSSMAQRQQKAVDLILKDPDVAAVTSFIGIDGTNSTLNTGRMQIALKPPGER
                     SDNAQEIIRRLGQSLRKLPDIQVYMQAVQDLSIDDRVSRTQYQMTLSDPDGTELAQWV
                     PKVVDRLRALPQVADVADDLQDGGLQATLVIDRDAASRLGVTTAAIDNALYDAFGQRL
                     ISTIFTQSTQYRVVLEVAKPFRQSPSSLDHIYVATTSGTPVPLTSVAHVQQSNTLLSV
                     ERLGQFPATTISFNLAQGVALSDAVDAIRQAETGLSMPASIQTEFQGAAKAFQASLDS
                     TLWLLLAAVAAVYVVLGILYESYIHPITILSTLPSAAIGALLALLLTHTELDMIGVIG
                     IILLIGIVKKNAIMMIDFALEAQRQRGLSARAAIHEAALLRFRPILMTTLAALFSAIP
                     LMLATGAGSELRQPLGLVMVGGLLCSQLLTLFTTPVIYLFFDRLARRARAARHTSRVK
                     SGIRP"
     gene            complement(26198..27508)
                     /locus_tag="VFR20_02780"
     CDS             complement(26198..27508)
                     /locus_tag="VFR20_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HEU0229472.1"
                     /translation="MSQPSAKPKSPRQGLSWVALFILVIAFGAYWHFSHKPGATAGNG
                     SGHGGRGMAGAMATPVNVATVKQGLIETTIKAIGTVTALNAVTVQSRVSGELEGVYFK
                     EGQEVKKGALLAQIDPRPYQVALDQAIGTEKQNQALLENARRDLKRYTLLYKQNSLAG
                     QQLDTQKALVQQYLGTQTSDAAAVASARLQLTFTRITAPITGVVGLRQVDPGNLVSAS
                     GTQGLVVITQMRPISVIFSLSQAQLPPVLAEVRAGKKLEVDLYDRSGVNKIATGTLAS
                     IDNQIDVTTGTVKLRATFANENEALFPNEFVNAVLHVSADDHAIMIPTSALQQGSIGA
                     FVYLVKDGKVHVQPVKTGTVDGTDVAVSSGLSPGQQVVTVGVDRLREGAAVKVIEASD
                     ATAGKTAAGKAMPGSAASGGAVAPPEGGAAGTTKSGSSRGTGHR"
     gene            complement(27687..28439)
                     /gene="zapD"
                     /locus_tag="VFR20_02785"
     CDS             complement(27687..28439)
                     /gene="zapD"
                     /locus_tag="VFR20_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HEU0229473.1"
                     /translation="MILYEYPCNERVRALLRVEYLFDRLFYFVEGDDVRHHQVAVATL
                     FDLLDISDRTDLRGAVLQDLERQRVTLGGLRQHPGVDVDTLDTMLAEIQEVSGNLGSQ
                     GRIGQVLRDNEWLASLRGRVAVPGGSSPVDMPSYYAWQIKPRESRCNDLRQWIEPFLS
                     LHQGLALILRLLRDSSTPTPLEAEQGAYQEMLGGKTFQLLRVWVDPSLQVFPEMSANK
                     YVIWVRFSTADQELKPQPANRTIPFHLSRCNV"
     gene            complement(28474..29100)
                     /gene="coaE"
                     /locus_tag="VFR20_02790"
     CDS             complement(28474..29100)
                     /gene="coaE"
                     /locus_tag="VFR20_02790"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247556.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU0229474.1"
                     /translation="MGMYSIGLTGGIGSGKSRVADMLHDGGAAIIDTDAIAHSLTASG
                     GAAIEPLRREFGGRIITADGALDRSVMRELAFSDMSVRQRLEAILHPMITSVTQAEAA
                     RAQGCYLVYVVPLLVESGRWRDRVDRVCVVDCDEPTQIARVRVRSGLTPEAIARIMSV
                     QASREARLAAADDVVFNGEGTSLDDLAQRVRLLHAAWRLQAAHRQALS"
     gene            complement(29186..29884)
                     /locus_tag="VFR20_02795"
     CDS             complement(29186..29884)
                     /locus_tag="VFR20_02795"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006713188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEU0229475.1"
                     /translation="MWPDLFSPAWHGARSAGSVADWMGLCLVLAGAVWAGRRIQRSFG
                     GARHSASRARVPLRMLCGVGVLFYAAVAGVFYWRHGWTPVFFGAFVAAGMLLALALID
                     ARTSLLPDALTLPLLWLGLAIAWLGGPVSLDDAVAGAMVGYGLLWLLFQAFRAVRGRD
                     GMGYGDFKLLAALGAWLGPHPALLALLAACLGGLLFACVRQKTWRPAGAYPFGPFLAL
                     AGAGFMLAQCALSG"
     gene            30013..30690
                     /locus_tag="VFR20_02800"
     CDS             30013..30690
                     /locus_tag="VFR20_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229476.1"
                     /translation="MPHLIQRFLRPVLHASLAAAFLTLAGCAQQRAPGYYAVQHDNTY
                     SDAQQQGQGTAQSKAPSQLQLGFGSQNQKANDNPAGNTESAVQTREWEATRPLREPKT
                     FLGTVPCLTSPGACAATRVTLTLAPSGEWRARSTPLGGNGSNTGGAVAQQGCWHVIGT
                     APLKILLQLNNLNPKATLTFINDNVLRIDSFNGVTPNLEYRLTRQADIDGIDELHGKP
                     VLDCKTK"
     gene            complement(30696..30932)
                     /locus_tag="VFR20_02805"
     CDS             complement(30696..30932)
                     /locus_tag="VFR20_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEU0229477.1"
                     /translation="MAAGGFDTDHPFFSTLTPRERDVMGYVALGKPNKIIAAELGVSQ
                     RTVEAHRARVFQKLRVRNAVELTRCVMSQHLPAG"
     gene            complement(31051..31893)
                     /gene="panC"
                     /locus_tag="VFR20_02810"
     CDS             complement(31051..31893)
                     /gene="panC"
                     /locus_tag="VFR20_02810"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEU0229478.1"
                     /translation="MKVVHTIEELRDQLRGQQRVSFVPTMGNLHAAHLSLMKLARQHG
                     DPVVASIFVNRLQFGPSEDFDRYPRTLQADIEKLERERDVYVLFAPDEHEMYPEPQNY
                     KVLPPDDLGGILEGEFRPGFFTGVCTVVTKLFACVQPRVAVFGKKDYQQLMVVRSMCR
                     QLQLPVDILAHETVRDTDGLALSSRNRFLEAAERAEAPRLYRVLQDLRDQVRGGATDG
                     AALEARACDELAAHDWRVDYVSLRRQRDLNPPQAGEIEAGEPLVVLAAAKLGATRLID
                     NLEI"
     gene            complement(31933..32787)
                     /locus_tag="VFR20_02815"
     CDS             complement(31933..32787)
                     /locus_tag="VFR20_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEU0229479.1"
                     /translation="MADLNALVDPAVDSTPDIVDSVALARLYGEPLFAIPQDLYIPPD
                     ALEVFLEAFEGPLDLLLYLIRKQNFNVLDIPMAEVTRQYLSYVEQIRSQNLELAGEYL
                     LMAALLIDIKSRMLLPITKSDTGEEPDDPRAELVRRLLEYEQMKLAARKLDALPRAGR
                     DFQRSHALPDLQVERMLPEVSPDDLRQAWVDIMKRASLNASHRVTREQLSVRDHMSRI
                     LRRLGDVRFLEFSELFMEGVREGDPAAVVVVHFLAMLELARESLLEITQAEPYAPIYV
                     RLSYVPSA"
     gene            complement(32825..33016)
                     /locus_tag="VFR20_02820"
     CDS             complement(32825..33016)
                     /locus_tag="VFR20_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEU0229480.1"
                     /translation="MAKNYESEITQFLKKYKTENPGTDARQRAGRARLWDRQLDSEAL
                     DGFRSARVAQRPYVYYDME"
     gene            33081..34541
                     /locus_tag="VFR20_02825"
     CDS             33081..34541
                     /locus_tag="VFR20_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014486113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEU0229481.1"
                     /translation="MPANPLPAPPTSLSPRERSAIRMLPFIVGCALFMQMLDSTVVAT
                     ALPVMATALGSTPVQMNVVITSYLLAVAVFVPISGWAADRFGSKRVFIAAILLFTLSS
                     IGCAASQNLVELVVARVFQGMAGAMMVPVGRIILLRRVPKSELLKAMSFLSLPALLGP
                     IIGPPLGGFLVTYASWHWIFLINIPVGILGIGLVTYYVRDDYPAGAPRLDWVGFILSG
                     ISLATLVSGFESLGHQAGGALQSLVLIGTGLACGALYCWHARHTAHPIIDLSLLRVRT
                     FAVATLGGNLCRFAVGASPFLLAILLQVGFGLSPFSAGMITFTGAAGAILMKLVATPI
                     IQFFGFRRILTINALIAAVFISLCGLFRSDTPVWIMVVILVSGGFFRSLQFTAVNTLT
                     YADLGSQEMSRASSFAAMAQQLGISLGVGCAAAVLNLSMRWRGDTHLALPDIIAGFLF
                     IGAVTAASYLSFRRLEPGAGDHIHKNRKVARASPTT"
     gene            complement(34623..35432)
                     /locus_tag="VFR20_02830"
     CDS             complement(34623..35432)
                     /locus_tag="VFR20_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0229482.1"
                     /translation="MPPLRVPEAQAGQARRRALGDFLRSARARVTPEMAGLPAGVRRR
                     TPGLRREEVAQLCDISVTWYTWIEQGRGVTVSPGVWTRLAAVLRLARAERHYLFDLAE
                     CADPEHGGMNPEPLPGDLAGCVHSIAAPAYILDRCWNVLARNDALLALFDGWPDRSDR
                     PNLLRYIFLDPAARSLIVDWDQRARRVVAEFRADAGPHADDPGVREVLDELQAGSREF
                     AHWWTRQTVVEREGGLREFNNPARGLLAFRQITFRMAIRPDCKLVMLMPEA"
     gene            complement(35462..>37801)
                     /locus_tag="VFR20_02835"
     CDS             complement(35462..>37801)
                     /locus_tag="VFR20_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEU0229483.1"
                     /translation="HQARLCRRYGAAVVVMAFDEKGQADSLQRRIDICSKAYHLLVDE
                     VGFPPEDIIFDPNVFAIATGIDEHNHYAVDFIEATGWITKNLPHARVSGGVSNVSFSF
                     RGNEAMREAIHTVFLYHAIKNGMTMGIVNAGQLGVYEDIDKTLRDMVEDVVLDRVEPV
                     GRTDPADERTSTERLVELAETIKGGGARKEEDLAWRAGPVQERLTHALVHGITSFIVE
                     DTEEVRQAIAARGGRPIEVIEGPLMDGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAH
                     LIPFIEEEKRQIAEAGGDVRSKGKIVIATVKGDVHDIGKNIVTVVLQCNNFEVINMGV
                     MVPCAEILAKAKAENCDIVGLSGLITPSLEEMAYVASEMQRDDYFLSRKLPLLVGGAT
                     TSRVHTAVKIAPNYEGPVIYVPDASRSVGVATSLMSDQAGTYLAEVAEEYELVRKRHA
                     SRKTTPLISLEKARAARPVIDWSAYTPPRPKFLGRRTFKHYDLTEVEKFLDWTPFFQT
                     WSLFGQYPAILDDKVVGEQARKIFAEGRDMLRKVIDGRWLTANGVVGFYPANSVNDED
                     IEVYRDESRSEVLLTWRNLRQQTEKREGVDNKCLADYIAPKATGVADYIGLFAVTGGI
                     GIEKHDERFLADNDDYSSIMLKAVADRLAEGFAECLHARVRRDLWGYVPDENLGNDDI
                     IAEKYQGIRPAPGYPACPEHVVKKDMFRVLECDDIDMMLTDSYAMYPASSVSGFYLSH
                     PMSQYFNLGNIGDDQVRDYMRRSGRTEDDVRRTLASALR"
BASE COUNT         7154 a        12286 c        11574 g         6787 t
ORIGIN      
        1 acattctcga cttttaaaac aatcagcaga tcacactggg ttgccagggc acgaactcgt
       61 tttgtccata gccatagatc tcgctctttg agcgctcgcc ggaggcgcag cggcgtatca
      121 tctcgtagaa ttcccgcccc agttgctgaa tatcggcacc ttcgtccacg accgttccgc
      181 aattgatgtc catgtcgtcc cgctgacgat cccacaagac ggtgttggtc gccagtttga
      241 gcgacgggga cggcacgcag ccgaatgcgg aaccacggcc cgtcgtgaag cagatgatgt
      301 tggcgccgcc cgccacctgg ccggtggcgg acatggggtc atagcccggc gtgttcatga
      361 acaccaggcc gggggcctgg acaggttccg cgtagtcgta gacttccatc aagcccgtcg
      421 tgccgccttt ggccacgcca ccgagcgatt tctccagaac ggtggtcagc ccccccttct
      481 tgttgccggc ggacgggttg ttgttcatcg acgcgtggtt gcgatggcac acgtcttccc
      541 accatccgaa aatatcgacc agtttcttgc caacagcggg cgtcgccgcg cgacaggtga
      601 gcaggtgttc cgcgccgtag atctcggggg tttccgaaag aatcgccgtg ccaccgttgc
      661 gcaccaggat gtccacggcg gcgcccagca ccggattggc ggtgatcccg gagaaactgt
      721 cggaaccacc acactgcaag ccgattttca gatgactgac ggggacgggc gtgcgttccg
      781 ccctgttggc ctcggccaac atgcctttga ccagctccac gcccttggcg atgctcttgg
      841 gcgtgccgcc gacgtcctcg atattgaacg tatgcaggtt gctgccttcc cgcagcgcat
      901 gcgccttcat catgaccgaa atctggttgg tctcgcagcc gagaccgata atcagcaagc
      961 ccgcgaaatt cggatgccgg acgtatccgg cgatcgttcg ctgcagcacc ttcagccctt
     1021 ctccggacgc gtcgatccca cacccgacgc cgtgggtcag cgccacgacg ccatccacgt
     1081 tggggtaggc cgccagcgct tcaggatgaa tatcgcgcct gaactggtcc gcgatggcat
     1141 gcgcgaccgt ggccgagcag ttcaccgacg tcaggatgcc gatgtaattg cgcgtcgcca
     1201 cccgcccatc gggccggacg atacccatga aggtatcggg cttgtcggcc acgcgggtcg
     1261 gcacatagtc ttgtgagaac cgatagtcct tgtcgaactc gcggaactcc atgttatgca
     1321 tatgcacatg ctggccggcg gcgatatccc gggtggcgaa cccgatgatc tgcccgtagc
     1381 gtctaacggg ttgcccactg gccaccgccc tgagggcgat cttgtgcccc agcggaaccg
     1441 ggttgagcag ctccaggttg ttgacgcggt caccctttga caggtcccgc agggcgatca
     1501 tgacatcgtc cgcgggattg aggatcagga atttttcgct tttggacatg gctttcaatt
     1561 acttcagcat gttctcgggc aatgactgac cgaagaaata tttcttgtag atcgcattga
     1621 gctggccatt cttgagattg gtcgacaccc atttattgat gcgggccttg aatgccggct
     1681 cgttctggcg aatgcccacc cccatcggga actgcgtggc cgtgtatttg taggcaagat
     1741 cggcttttgg attcgccttg aggatctccg gcaacacgct gggagcgccc accagcactt
     1801 tctgctgccc cgtcgacgcg gcggtattgg acgtggcgtc gtcttcgtag cgaacgatcc
     1861 gcacccctgg aacacccttc acggaatcag tgatcgtcat gtcgctggtc gtgccgcgcg
     1921 tcaccgcgat cgacattttg gccagatcct tggggccggt gatcttcgcg gccttgggcc
     1981 cgaacaccac gacgggaacc acgccgtacg gttcggaaaa gtcgatgacc ttcttgcgtt
     2041 cggcggtgat cgagaacgac gccatgatcg cgtcgacttt gttggtgcgc aagtactgca
     2101 cccgggtcgg gccggtcacg gggacgatca ccagcttcac ccccagatcc ttggcaagca
     2161 ggcgcgctga atcgacatca aaccccgtct tcttggcatc tttgtcgacc atgccgtaag
     2221 ggggggcgct gatgtcgaca ccaattcgga tctcgccctt tttctgaatg ctggtcagct
     2281 cgttcgcctg cgcggtgaac gcgcaggccg tcgccgcgat gcaaagggcc agcgtgggaa
     2341 gggtcttgaa aatctgcatg tcttgctccg ttgttatcgt ttgaaaaaac ttcgtgatga
     2401 atgggatatg gccgctcaca ggccgttgct gatgaactgc cgcagttcgg gggtggccgg
     2461 attcttcaat aggtttcgat caccggactc ccagacaacc ccctcccgca tatagatgat
     2521 ctggtccgcc acggccgacg cgaaacccat ttcatgcgtc accagcacca tcgtcatgcc
     2581 gcccttggcc agtccttcga tcaccttcag cacttcaccc gtgatctgcg gatccagggc
     2641 ggatgtcacc tcgtcgaaca gcatcaattc gggttgcatg gccagcgatc gggcaatggc
     2701 gacacgctgc tgctgccccc ccgagagctg gtcgggatac gcatcgatct tgtcggacaa
     2761 ccccacctgg cccagcacat gcgcggccag ttcctgcgct tccttgcgcc cgacgttctt
     2821 gaccttgcgc ggcgccagcg tgatgttctt ttcgacggtc aagtggggaa ataggttgta
     2881 gctctggaac acgatcccga cctgcagcct gagggccggg atatcgcaat ccttcgcatg
     2941 caacgcccgg tcgcagacgt acagctcgcc gccatcgatt tcctccaggc cattgatgca
     3001 gcgcagcgcc gtgctcttgc cggagccact ctggccgatc agggcgacga cattgccttt
     3061 ggggacttcc agtgagatgc ctttcaggac ttccagatcc ccaaaggatt tcctgacgtt
     3121 ctggaaacgg actcgcggac tcataaaacc ctcacgacct ggaatgccgc ttgagcttgc
     3181 gctccagacg gtacgacagg attgacagcg gatagcacat ggcaaaatag atcagcgccg
     3241 ccacggtgaa cacgacgaac ggcttgaacg tcgagttgtt gatgacgcgc gacgaatagg
     3301 tgaggtcgaa gaacccgatg accaccgcgt acgacgtgtt cttcacgatc tggaccagaa
     3361 agcccacggt ggggggaatc gcgacacgga gcgcctgcgg caatatgaca tgcgccagga
     3421 cctggaattt cctcagggcc aggctttccg aggcttccca ttgggtgcgg ggaatggctt
     3481 cgatgcagcc ccgccagatt tcccccagga aggcgctgga atacaacgtc atggcgattc
     3541 cggcggccac cagggcgggc agatcgtacc cggcgatgct gataccgaaa tagcagatga
     3601 acatgaccac cggcagcggg atgccctgga tcagcttcac atagccggcg ctcagaattc
     3661 tgaaaaacca cccgtgggac gtccgcgcga tggccacagg caaccccacc accgtaccgc
     3721 cgacaaagcc gagaatcgac aacaccagcg tccagccggc accgatcaga atgaacagcc
     3781 attggctgta cgtcacaccc aggatcatga cttcacctcg ctggctgtcg cgccgccgac
     3841 acggcccgtt ttcacccgtc cgcccacgga cgatctggct cttgcgaggt gccgttgttt
     3901 cgggaatgcc aggttgtagc aacccgccat gacaagcctc agcagccagg acatcaccag
     3961 ataaatgacg gcgatgacca cataggcctc gaaaccacga aaagtccggc tctggatctc
     4021 cagcccgatg gccgtgagtt cctcgacgga aatctgcgac atgatcgacg aggccagcat
     4081 catcagcacg aactggctga tgagggacgg gtacatcttt tctatcgcct ggggcagcac
     4141 gacgtggcgc atgacctgcc cgcggcgcag acccaggcac gcggcggctt ctttctgccc
     4201 atagtgcacg gcgtcgatcc ccgcccgcac gatctcgcac gtataccccg aggtgttgat
     4261 ggacagcgcg accacggcgg catagaacgc gggcaccagg aagcccagcg ccgagagccc
     4321 gaagaacagc cagaaagtct gcacgatcaa tggcgtgttg cgtgtcagat ccacaaagcc
     4381 ggcggccagg cggcgcgcaa gaggcgagcc ttggctacgg gccacggcca gcgccacacc
     4441 gagcacgaat ccgccgatca aggaaatgat ggacagctcg gtcgacaccc aaaggccatg
     4501 gagcaggctg ggccaatacg cgccgatgaa gctgaagtcg aagctgtagt tcatgccgaa
     4561 tggttcttgt caccgtgttt tgtccacctc acgggaacca agtatccatg acgcgcattc
     4621 ggcgcgtcga acgaataata gtggtcggct gtacgctttt tggaatggcc gcccgcccca
     4681 cacaggagaa gacggggttt ccgacgtcaa acagtcgacg agactacggg taaaccatga
     4741 tctcccttgc gctcaagtcg gcccacggcg ataggtaccc gccacagtgc tcctgtaccg
     4801 gcccgatggt ttaactatac tcatacccca tccacccgct ctcggcggtg atagtcatgt
     4861 ctgccgcctt gacagcaccg gtgcgttggt tcaaaaccgg attcaccctt tggaggctac
     4921 atgagtatca agaccattta cgttcaggcc ggtggcggct tcgacaaggt aatgatgggc
     4981 gcaagcgagc ccaggccccc aggtgtcggc gaggttgccg tgcgcatccg cgccagttca
     5041 ctgaactatc acgacttggg tgtggtgagc ggaagaatgc cgcccagcga gcagcgcatc
     5101 cccatgtccg acggcgctgg cgagatcatc gccgtgggcg cgggcgtcga ggaattcgcc
     5161 atcggcgatc atgtggtcag caccttcttc cccgactggc tggatggcaa ccccaggcat
     5221 gccgggtttg gccgtacacc gggagacggt atcgacgggt acgcgcgcga acaggcaacg
     5281 atgcccgcca ccgcattcac gcatgcgcca cacggctata gccatgcgga agccgcaact
     5341 ctcaccaccg ccggcctcac ggcttggcgc gccttgttcg aaaacggcca actgaagtct
     5401 ggcgaggccg ttctggttca aggcacgggc ggggtctcca tctttgccct gcaactggcc
     5461 aagcgcgcgg gagcgcaggt gatcgcaacc tcgtccagcg acgaaaagct cgataagctg
     5521 agggcgctgg gtgccgacgc ggtgatcaac tatcgtgacg accctgaatg ggggacaacc
     5581 gtccggcgcc tgaccgatgg tcgcggcgtc gatcatgtcg tcgaggtcgg cggaccagcg
     5641 acgttggcac agtccatcac cgccagtcga gtgggcggcc atatcgccgt gattggtgtg
     5701 cttagtggtc gcaccagcca gctgccgttg tcgcaggccc tgtcccggca ggtgcgcctg
     5761 caagccatac tggtcggcaa ccgccgccaa cagcaggatt tcatccgtgc catcgaagcc
     5821 acgggcctgc gacccgtgat tgatcggcac tttcccttgg acgagatcgt tgccgccttc
     5881 cagcaccagg ccagcgggcg gcatttcggc aaaatcgtgc tcgatatcta gggagcctct
     5941 gaacaaatca ccgcgcttgc ttgccgctcg acttgttcag aggctcccta gtgccgtgtt
     6001 cgatgcctgg ctaatttgaa cacgcaactg cggggccgga acctgccatg catccgggat
     6061 gctggcggcg gtatttgagt gggcggagcg gtgacgctgg aatgtgctgc ggacccaagc
     6121 acgtccgtcc cgcttcatat agcctgcgaa aatatgttat gtgttcgcaa agcaaacgcg
     6181 tagaataatt cctcaaatgc ggcctatcgg attttttgga agcttctcgc tgacacacaa
     6241 ccggagccaa agacgccatg aagcacttca ctctcaaagt caacggtcgc gagcagaaag
     6301 tggaggcgga acccgactcg ccgctgcttt atgttttgcg cgacgatctc aacctcaagg
     6361 gtccgcgttt cggctgcggc ctggggcaat gtggtgcctg caccgtaatc atggatggca
     6421 acgcaacccg ctcatgcgtc tttccggtat cggcggcggt cggcaaagag atagtcacac
     6481 tcgaaggcct tggctccagc cagcatccac ataaaatcca gcaagcgttc atcgacgcgc
     6541 aggccgcgca gtgtggatac tgcatcaatg gcatgatcat gacggccaag gcattcctcg
     6601 acaagaatcc acacccgagc gccgaggacg tgcgccaggc tctagcctat aacctatgtc
     6661 gatgcggcac ccatgtgcgc atagtcgatg ccgtcatgct cgcggcaaaa gaggcatagc
     6721 atggacaacc acctcaattt cgaccttacc cgcaggcagc ttctcaagac gggcggctgg
     6781 ctggcggtgg gcttctcctt cgcgggcccc gttgcgcaag ctgtggcggc cgcccacggc
     6841 acgacaaaac cgaaagactt caagaaagtc gacgcttggc tcactattga tcgcaatggg
     6901 catgtcaccg cctacaccgg caaggtcgac ctgggtactg gcgtcgaaac cgctctggcc
     6961 cagattgttg ccgacgagct cgatgtgccg ttccagtcag tcaccattgt gcagggtgac
     7021 acgcaactca cccccgatca agggccgaca tggggcagcc tcaccattca ggccggtggc
     7081 gcacaaatgc gcaaagccgc cgcatgcgca cgtcgcgcca tgctgaacct cgccgcccgg
     7141 aaactcggtg tggcggcaga tacgctcaag gttgaaaatg gcttgatcca tagtggcggc
     7201 aaacagctcg cctactcaga ccttgccaaa ggcgacacac tggatgtggc cttcgatgac
     7261 aaagtgccag tgaaacaacc agatgcctac tcgattgtcg gcaagtcgat tgctcgcgtc
     7321 gacatccccg gcaaggtcac tggcgagttc actttcatgc aggacttcaa gttgcccggc
     7381 atgctgcatg cgcgcgtcgt gcgcccaacg gggatggccg ccaatcttga gtcagtggac
     7441 gacagtgccg cccgcaaagt gcccggcttc gtcacgaccg tggtcaaggg caacttcgtc
     7501 ggcgtagtgg cacggaccga atggggcgcc atccgcgcct ctcaagccat caagtgcaag
     7561 tggtcggact gggctggatt gcctgcgatg gcgcaggtct atcaagatgt gaaaaagacg
     7621 gctgtcaagc aaaccgtcac cccgaaaaac gtgggcgatg cgcccggcgt catccggtct
     7681 gccgcgaaat cgctcaaagc cagctacgaa tatcccgtgc agacgcacgg ctcaatcggc
     7741 ccatcgtgtt ccgtcgcgga catcggcaag gatcatgcgc gaatctggtc ggcctcgcaa
     7801 agcccgcatt ggctgcaggg ccagatcgcc aagatgcttg gtttcaaacc cgaacaagtg
     7861 cacatcatct atatcgaggg tgcaggctgt tacggccgta atggccacga ggacgcctcg
     7921 gctgacgcgg cgctactgtc gcacgcgtta ggcaagccag tgcgagtgca atggatgcgt
     7981 gccgacgaac acggatggga ccccaaggcg ccaccctacg tcgccgacat ggaaggcgca
     8041 ctggacgagg ctggccatgt gcgagcctgg aatttcgtca cgtggctgcc agcccgtatc
     8101 ggtggtttgg ccaatgtccc gttgctggcg gacagccttg agcacaagga tgcttcggtc
     8161 caaacagcag acaacatgaa cgccggctcg atggaaaacg atgccgaacc gccctatagc
     8221 ttcccgaatg tgctcgccac ggtccacagg ctggaaacca cccctttccg gccggcttgg
     8281 ctgcgtggtc ccggtcgcct tcagaatacc tacgccaatg agtgtttcat ggacgaactg
     8341 gccgccgcgg ccggcacgga tccgatcgcc ttccggctca gccacctgaa ggatgaacgc
     8401 ggtgccgcgt gcatccaggc ctgtgtcaaa caggcaaatt gggtcagtcg cgcatcgcca
     8461 caaaaggaca ccaccggcga cgtgctcaag gggcatggct ttacctacgt gttctatgac
     8521 aacagtcgca cgtatgtatc ggcaatatgc gaggtcgagg taaaccgtgc caccggcaag
     8581 gtgcgcgtca cgcgcttcgt gatcgcacac gactgcggcc tcatcgtcaa ccctgacggc
     8641 gtcaaggcac agatcgaggg caacgtcatc cagacgacat cacgcaccct gctcgaggaa
     8701 gtcaagtggg acagttcgca cgtgaccagc cttgattggg ccagttaccc catcctcact
     8761 tttcccgacg tgcccaagat cgagatcgtg ctcatcaacc ggcccgacaa gccagcctgg
     8821 ggtgcgggcg agccgacctg tgcggtgatc gcccccgcca tcgccaacgc gatcttcgac
     8881 gccactggca agcatgtgcg cacggtgccg ttcacccccg cgcgtgtcaa ggctgcgctc
     8941 acgacataaa caaccccgac ctgacttcgc ccggtttccg tgcgcgcttg cgcaaaaagc
     9001 cgggccgcta tttgccgcgc atgcctgcca ccatggcggg cgtcacgtcg cccttgtgcc
     9061 cgccccatgt cacacgcagg taattcgcca ggtcggccat ggcctggtcg tcgagccggg
     9121 gcgcaaaccc cggcatggcc tgcattgccg cgttgcccgt gaacgtctgg gcgccgatgc
     9181 cgtcgagcat gacggcgacc agattgtggg gatcggcatc gcgcagcgta ctgttggccc
     9241 gcatcgcgac cgacacgttg ggtttgccct ggccgtcggc ggcgtggcag ccggcgcaca
     9301 gggccagata atgcagacgc cccgcctcga cggtctggcc ggcggcggcc ttggccagcg
     9361 gcgccggagc cggcggcgag tcgccggtca ggtacgcgac cagcgccgca ttgtccgctg
     9421 gtgtcaggtg gcgcgtgctc aggtggaaca cctcgtacat gtcactgtag accgagcctt
     9481 gcggcgccac cccatgcgcc aggaactgct tcaggtcctg cgcgctccag ccgcgcgcgg
     9541 ccagcccagc gggcgtgatg tcgggggcat gaatccggcc caggtcgccg ccggccagcg
     9601 tggcgcgaag gtcgagctgg cccagggcgc cgcgtgccgt gtggcattcg gtgcagtgac
     9661 ccagcgcatt ggccaggtag cgccccctca cccactgcgt cgaggcgccc ttggacgcat
     9721 ccggcagaga atcccccagg aacaggaaat tccagccacg catcaaggca cgcgcggaat
     9781 acgggaaggt gaaagtgttc ttcttgtcgg gctgcggcac cgccctgagg ctgcccagat
     9841 aggcgaacat cgcgtcgctg tcctggcgcg tgatgccgcg gtacgacgtg tacggcatgg
     9901 ccgggtacag gggatgaccg ggggccagcc cgtcgtgcag ggcacgatag aaatcggccg
     9961 acgtccactt gcctatgcca tgttccttgt cgggcgtgat attcgaccca tagatgcggc
    10021 cgaaaggcgt atcgagcggc acgccaccgg caaagggcgc accatctttc gtcgtgtggc
    10081 aggccgcgca gtcggcggcc cgcaccaggt accgaccttt ggcgatcaat tgctgcgaag
    10141 gcggcgcctt gtcgacgtcc gacgtggcat agcctgggtc gaacgccccc gagagcaggg
    10201 cgcccgccgc aaccaccacc acgaccacgg cgcccaggaa gtatgtcttc ttacttgcca
    10261 tgagcggact ccttgagcag gtccggcgtc gccaggatca ggtctttcac ggcgttgtgg
    10321 taacgcacgt agccggtgca gcggcagata tggtcgttca gggcatcggt gaccacggcc
    10381 tcgacctggc ttttctcgat gggatgacgc ttgagttttt cgagcagcac cgtggccgca
    10441 ttgacgaacc ccggtgtgca gtagccgcac tggaagctgt agaaatccag gaacttctgc
    10501 tggacgggcg acagcgcggt gacctcgcct tcctggttgc gctgcgcatg tccctcgatg
    10561 gtgcgcagct tcctgccctg gaaatacagg gcaccggtga tgcaggtgcg gatctcttca
    10621 ctcgtgccgt cttcgtggtc caggatggcc acacaggcgt ggcagatgcc ctgcccgcac
    10681 cccaggcgcg aaccggtcag gcctgcgtat tcgtgcagga agtcgatcat catcaggctt
    10741 tcgggcacct ggaacggccc caccggcttg ccgttgatga tcattgacag ggacttggtc
    10801 gcgatgctca tgccagcacc tcctggattt tttccgccgt gacgggcaaa gacatgaatc
    10861 gatggcccat agcgtgcgcg agcccgttga cgatcgcgcc cacgatcggg atcatcacga
    10921 cctcccccat gcctttcggc gggtcggtat ccgacaacgg cggcagcact tcggccgtct
    10981 ggctccacaa ggccacttcc ttggccaggg gcaggtggta gcggttgaag ttccaggtgc
    11041 cgttgcccgg accgtcttca tacagcggca aatgctcata tagtgcatgg ccgatgccca
    11101 tggcgatgcc accctgtatc tggcccgaca ccagctcggg cgacagcatg ttgccgcact
    11161 cgatgatgga atgatggttg agcagttcga ctttgccgtt gccacgatcg acggtcagtt
    11221 cggccagggt gcccatggcg ctgtaatagg tgacccccgc attgttgcgc tgcacgggcg
    11281 ggtagtatgc tgtctggcgc cggacgatct ggtagcctgg ccccgctttg ccgcccgagg
    11341 ccgccgcgcc gccgcccttc ccatacaaca gtgccagccc gtcgagcggc agcctctcgg
    11401 tcttgccgcc aatgggaaag tcggcctcgg cccattgcca gcgattgaac ccatgcacca
    11461 ccgcgcccac gaccaacccc atgtcgtgag ccttctgagc caattgcgca aggcctagcc
    11521 cctgcatgcc gttggctgtc agcaggccat cttcccagcg ggcgtcctct tccctgacgg
    11581 tatagggcgc ggcttggccg ccgcccaggc cttggctcca gattgccagg gcggccggcc
    11641 acaggccatg ccggaaaatc aggcgacagg cctcgcgcgt ggtgtgcgtg aaatagaacg
    11701 ccgaattcga cgcgctggcc ggcgacgcga tggccggtgt ccacatcgga tcggcggcgg
    11761 cgcggtcctg gtcggcctgg ctggagaggt acggatcgcc actggtcttc atgggcagtt
    11821 gaggccagtc ggtgatggcg tagcccaggt cggttgctgg cgaccccagc cacttgacac
    11881 aggcgatagc ctgcgacgtg ctcatgccgg tgccgatctc ggtgccggtg tggcgcagca
    11941 gcacccggcc gtcaggcgag acctcgaccc tggccgatga cgactcggcg cctgtcccga
    12001 agtctttctg cacgcaggcg aaccctacgc cgtacagctt gcccggatgg ctggcctcgt
    12061 aggctttttt gcgcttttca cggtcgcgcc acagcgggtg ctcgcgcgcc ttcagcagga
    12121 cttcgtcggc gcggacctgg cccgccggca cggcaccctg cgtatttttc atgccggtct
    12181 tcaggacgtt cctgaggcgg aattcgatgg ggtcgagctt cagttgcccg gcgatttcat
    12241 cgaccagcat ttccgtggcc gccatgcttt gcagcgtgcc atagccgcgg gccgaaccgg
    12301 catcgacggc gcgcgtggcc tcggcgcggg aggtcaggtc gctttttggc agatagtaga
    12361 tggtctgggc ggccgtggcg cccaccatgg ccaccgatgg cgaaaaattg cagcgcccgc
    12421 cgccattggc caccatgtcg gccacgaatg tccggaacag gccggtcttg cggtccacga
    12481 tcacctggta atcgatgtcg aaggcatggc gcttcaacga cgactggaag tgttcgtagc
    12541 ggtcattggc caggcgcacc ggccgggagt ccgcatacag cgcgcacgcc agcccataga
    12601 acggcatgtt gcagtggtcc ttcgacccgt agcccacggt ataacagggg tgcagcacca
    12661 ccgacttgat tggaaacgcg gtcttggcca gcatttcgct ggcggtggat gcgacttcgg
    12721 tgggcgactg ggtcggcacc accatgtgca tgacctggtt ggccgcgtcg taccagcaat
    12781 tggcgttgtc gggttcgagc gccgacgtat cgaccgactg ggtgtgatac gtgcgccgca
    12841 gcaccagttc gtcctgtcgg gggttgtcga attcgccgcg aatctggccg gcgtagtaca
    12901 tgccttcctc gccaaggctg ccatggtctt tgccgtcggg ccagataggc aggtgcttgc
    12961 gcatctggct gggaaaaatg ggggcatcct tcaggctgga aaacacgtca tcgtcgtaag
    13021 gcgtctggcc gccgacgcgc acgaagcgga aagttcccca tgggtcgcgc tgcaacggac
    13081 cggtgcgcgc gccgtacttg atgacatcgg cctggaactg cagggcgctc tttgccaggc
    13141 ggaaacgcgc gaaatcgtgg aagatcagta ttgccaccgc atgccccagg taggccgggg
    13201 tcttgccttt gggcagcagc atgtcttcgc cgtagaaatc ggtgaacgcc accttgtcgc
    13261 gggccaggtc ttcggcggtg accacccggt ccggcttcag gtcggcaccc aggcgcgaca
    13321 ggtcgaaccc ttcgtagagg gcatcggcgc gcgtggcgcg caacatgaac gcgtggcttt
    13381 gccgatcagg ccagtgggga agatcgcggg ctcggatgtc gcgcgcgaag attttcgagc
    13441 cggttacctt ggcgaccccg tcgacccgga attccatctt gcccgccgca gcgtcccagc
    13501 gtaccggcgt gagcctttca tcgtcgaaca aggcggcata ggctgtcgag ccaaaaggcg
    13561 cgatataggc cgtgatgccg gcgaccacca ttgttttcac gaaccggcga cgcggcaggt
    13621 cgggctttgc catggacggg gtactcctct tctataatgc agccgttcag caaacgatat
    13681 accgtcaagc cacactacta caacggaaga atcaggcgca ataccctggc ggcggcaacg
    13741 accggaattt attgttggaa ttcatgtctg gaaacagccg cctgcgacca gcagccagtt
    13801 taggtccgca gagaaatgcg cgccggcata gcggcactca ctgcggaagc tggccgccac
    13861 cgcgccggaa gacagcgcat gagaaaggcc aatgaacctg cctcggccct tacttcccgc
    13921 cagaaccctg gctggggggc gtcaagttta ctggcaccct gatgatttca gacgctggca
    13981 agacgacact ttcaaagcca gcgatgcggc agaggctctc ccattctccg ctccaaacaa
    14041 gggcagccga gccgtcgcga cagcccgacg ccgaatgatg catgcacata cccggcctta
    14101 gcctccggcc ttgcagttcc tggccagaat ggtgttggtc ggcgcgccag tcgtgccggc
    14161 cgacgtctct tggcggatca gcatgttgcc attcacctgg ccggcactct ccagcgtgaa
    14221 cttgcccgtc gtccacgtgt acccattgcc cgtgaaggta tcgcccacca tgtccgtctt
    14281 gctgttggtg gcacgcgtca aggcaatagc ctggttgtca tagataacct gtgccgtgac
    14341 cggatcattg ccctggaagg tgtactgcac ctccaggttc accgtccgct ggccctgcgc
    14401 gctgcattga tattggaccg tcttttgcga cagctgattc atcttgtcgg ggttcccggt
    14461 cgtggaacat gccgccaatg tgacgcacag caccgcgccc agggcgccgc tcaaagggaa
    14521 tttcgtcatt gcacatcctt ttcaagactc caggcttggc agcctgctcc tgtttttttt
    14581 atagcacccg acaggacggc ttctgtaagc gcgagtgtca aagtggctca gactccaatc
    14641 gcccggcagg aaccgaaatg gctgtcacat ccgtgtttcc cctgggtatt ccggccgtaa
    14701 ctgtctgttt gatcgaacaa gagatgccaa gaacggtttc agaagaaaca aacagagcca
    14761 cgactgcagg aatacgtcac aacatgcccc gcctgcagca tcggggcgcc ggcgttatat
    14821 tgattggcga cccgcttgcc gcggctgccc cgacacgctg tccggcaaga ccaacgccaa
    14881 ccaagtcgac gcggcccgcc acgcgctgca ggcccaggtg cgccagctcg gccacgcgcc
    14941 cggatagcca ccacaacccg caggaccgga atcctgccta taggatatcg aaggtgcaaa
    15001 acatcgcggt acggctgttc agcgtcacac cggcgccacc gggtgcgcac atgttcatga
    15061 cccaagcggg cgatgaaaaa caaaaagccc tttcataaaa agggcttgat gaaaaatcta
    15121 ttggtcgggg tgacatgatt cgaacatgcg actcctgcgt cccgaacgca gtactctacc
    15181 aggctgagct acaccccgac ggctttcttc agcggtctcg ctcgaccgcg aaagagcaga
    15241 attttagcag ggaatccttg taaacggaaa ccgggtacgg ggccagcgcg tcgcgggcga
    15301 tgtcggcctc ggcctcggcg gcgcggcggg tgtagtcgag cgcgtcggtg tcgtggatgg
    15361 cgcgcgccac ggcggtgaag tcgccctcgc ccgacacgat ggcctggcgg accaggtcgc
    15421 gctgcgcggg ggtaccgacc tccatgacac ggatgagcgg cagcgtgggt ttgccttcac
    15481 gcaggtcatc ccccacattt ttgcccagcg catggacgtc gccactgtaa tccagcacgt
    15541 cgtcgatgag ctggaacgcc gtgcccacat ggcggccgta cctggcggcg gcctcgatca
    15601 ggccggcggg ggcgcccgcg acgatggcgc ccacttgcgt ggcggcctcg aacagcttgg
    15661 ccgtcttgta gcgcacaacc tgcagatagc gatccagcga aacgtcggga tcgtgcacgt
    15721 tcaacagttg cagcacttcg ccctcggcga tgacggtggt ggcgcccgac agcaccgaca
    15781 tggcgggcat ggagtcggcc tcgaccatca tttcgaacga gcgcgagtac aggtaatcgc
    15841 ccacgagcac gctggcggca ttgccgaaca cggcgttggc ggtatcgcgc ccgcgccgca
    15901 ggtcggactc gtcgacgaca tcgtcatgca ggagtgtggc ggtgtggatg aattccacga
    15961 ccgcggccag caggtggtga tgcgtgccgc gataacccag ggcgccggcc agcagcagca
    16021 gcagcgcggg ccgcatgcgc ttgccccccg cccccacgat gtagtctccg acggtgcgga
    16081 tcaacacgac gtcggaattg aggcgggcgc gtatcaccgc gtcgacagct ttcatgtcgt
    16141 cggcgatcgg gtgtgtgagt tcagcgagat tcaagacgta gtccgggctt tccaggtgcc
    16201 aaaaaatgaa tcgagcccta tgcgggcggc gcgcctgggc gggcgcgacg agcccgggcg
    16261 acaccggccg cgcggatcga gccttagatt atacgttctc gggactggct tacgcttgta
    16321 ccggaacacg gtgcacgccg acgcggcgtt ccataaaccg acgacatggc cagcgaaatt
    16381 tcgccataat gaaccctttg cccccacaaa aaccggagat ccgattgtga gcaccaccga
    16441 cctgaccaca ctgatcgacc gcgcggcgcg cgtgctagcg cagcttgaag cctggctgcc
    16501 ccccgcaccg ccccccatcg actggtcggc gcacgcattc caatggcgca agcgcggcac
    16561 gcgtggctgg ctcgatgccg tgcaaaacgt gtcactcatc catattgccg acctgcagca
    16621 tgtggagcgc cagaaaacca tcatcgaccg caacacccgg cagttcctca ccggcctgcc
    16681 agccaacaat gtgctcatga ccggtgcgcg gggcaccggc aagagctcgc tggtcaaggc
    16741 catgcttgcg gcctacggcg accgcgggct gcgtctcatc gaggtcgaca aatccgacct
    16801 ggccgacctg gccgatatcg tcgacctggt gtcctcacgc cccgaacgct tcatcgtgtt
    16861 ctgcgacgac ctgtccttcg aggaaggcga ggcaggctac aaggcgttga aatccgtgct
    16921 cgacggctcg cttagcgccg ccggcgagaa cgtgctgatc tatgccacgt cgaaccggcg
    16981 ccacctcatg ccggaataca tgagcgagaa cctgagcgcc aagcaccagc cggatggtga
    17041 gatccatccc ggcgaaaccg tggaggaaaa gatttcgctg tcggaacgct tcggcctgtg
    17101 gctgtcgttc tacccgttca agcaggacga ttacctggat atcgtcaatt actggctggg
    17161 gcagcacggc tgctccgccg acaccattgc cgcctcgcgc accgaggccc tgcaatgggc
    17221 gctcgagcgc gggtcgcgct ccggccgtgt ggcgcgccag ttcgcccggg actgggccgc
    17281 gcggcatgtc taaacccctg atcgaggtcg ccgccgggct gctgttgcgc tccgacggcc
    17341 agttgctgct ggcccaacgt cccggcgaca agccctggcc ggggtggtgg gagcttccgg
    17401 gcggcaagat cgaaccgggc gagtccaccc tggatgcgtt ggcgcgcgaa ctgcgtgaag
    17461 agctcggcat cgaggtgacc gcggccacgc cctgggtgac ctacacgcac gagtatccca
    17521 agaatcacgt gcggctggcg ttctgcaggg tgacggggtg gcgcggcgac ccgcgcgggg
    17581 tcgagggcca gacgctggcc tgggtcgacc cccacgcatc aataccgatc gcccccttgc
    17641 tgcctgcgac cgaaccccct ttgcgctggc tgcgcctgcc gaaccgctat ctggtctctt
    17701 ccataggcaa cgacgccggg ctgccagcgt ttctggaaaa gctggagcac gcgctggagg
    17761 gaggcctgaa actggtccag ttccgcgaac caggctggga cgcggccccg gcccattccg
    17821 gcctgagacg cgttctggcc gcctgccgcg cctatggggc acgatgcctg gtgaacagtt
    17881 gccatcccga agtctggtgg gccgaggccg acggggtgca ttggcgcgcc accgacgccc
    17941 gacggcaagc cgccatcatc gccgctggcg ccgcatccgc atccgcatcc gcatccgcat
    18001 ccaatacatc cgacacgtcg gccccgcatc aagcaaccgc tgcggaagcg gggcaaacgg
    18061 cgctacgctt ctttcccgca accgatccca cccgccgact ggtcgccgta tcggcgcacg
    18121 atgccgatga tctggccgcg gcccgcgccc tgggagcgga cttcgccgtg ttgggccatg
    18181 tcctggacac gccctcgcat ccaggcgttc caggcatggg ttggacgcgt tttgcccagt
    18241 tgcgcgagaa cgcgggcttg ccggtgttcg ccatcggcgg gcaatcggcc gacacagtgg
    18301 cggacgccac gcgccatggc gcgcacggca tcgccggaat tcgctatctg ggctcctgag
    18361 cggagccctg cgtcacggac gcgccattgc ccgcgcccgc cgaggtggtc ccctccttca
    18421 cgtctgtcga tgcggttccg atcgaacctg gcgccgcctt cgcaccgatc gtcgccgtat
    18481 ccatcgcagg cgctggtgcc cagcccccgc ccagcgccgc gatcaattgc acgctcgcca
    18541 ccagccgatc caccgtgacc gacaaggccg cgcgctcggt gctgagggtc gaggcctgga
    18601 ccaccgccac gctgaggtaa tcgatcagtc ccgcatcgta ctgattggtg gtcaggcgca
    18661 atgattcgcg tgccgccgcg agggcacggt tctgcgtgac ctgctgtcgc gccagcacac
    18721 gcaactgcac catgtagtct tcggcctcgc gcaaagcagt cagcgccgtc aacctgtacg
    18781 aggcgacttg cgcatcgtag gcggcctgcg cctgctccac ctgggctgac cgggcgcccg
    18841 cgtcgaacag ggtcatggcc agttcgggcc ccagcgacca gaagcgcatg ggggccgtca
    18901 gccaattgac ccattcgcca ctgcggaacc ccccttgcgc actcagggtg agatcaggga
    18961 accaggccgc ctgcgccaca ccgatcttcg cattcgcttc ggcggtgcga cgctcggccg
    19021 ccgccacgtc tggtcgccgc tgcaacaatt gcgacggcaa tcccacggga atgatcggga
    19081 cgataccgat agactgggct tcggccagtg aaaaccggga tggcggcttg ccgatcaaca
    19141 cggcgatggc gtgttcgtac tgcgcccgct gccattcgag cgcttgcatc tgggcacggg
    19201 cattctcgag ttgtgtctgg gcggatgcca catcggcctt ggccgacacg ccagcgttgt
    19261 atcggttcac cgtcaccttg agcgatcgct cgtaggcggc cacggtttgc tgatacatgc
    19321 gtttctcggc gtcgagcgcg cgcaactgga agtagtcttg cgccagcgtg gattccatgc
    19381 tcaggcgcgt cgccgccaca tcggccgcgc tggcctgcaa ttccgcctgg cccgcctcga
    19441 cggaacgccg gacttttccc cagaggtctg cttcccaggt gaccgtgccg ctcaacacgt
    19501 agctgctcga cgtaccgccg ccggaaaaat tggaagagcc cgactgaccc gtcccgccgc
    19561 ctgcgcgtgc cgccgaggac gacagaccca cggtgggaaa taggcccgcc cgggtggatt
    19621 caagcgccgc cctggcttcc cgatactgtg cctcggactg ggccacgctg aggttggcat
    19681 cctgcagcgt gtgcatcaga ccatccagca ccggatcccg atacagacgc caccaatcgt
    19741 cgtgcaacga cacaccagcc ggctttgcct gcacccagcc tttgacctct ttatattgct
    19801 ggcccatgtc tacagcaggc cgatgataat ccgggccgac cgcgcagccg gccaacagca
    19861 ccgccataca gaagaccgcc agaccacgtg cgccagacaa ccgcacactg acgccgtggc
    19921 ggcccgacga gcggcgcccc tcatacacgg atgcgcgacg cgcaggggac ttgtccagag
    19981 gatacttagc catgtttcgt tattcctcgc ttgcgattcg cccaccagcg gaagcgatcc
    20041 aggtacagat agaccaccgg ggtagtataa agagtgagta tctggctcag aatgaggccg
    20101 ccgacaatcg tcaaccccag cggccggcgc agttccacac cggcaccgga cgccagcacc
    20161 aggggcagcg ccccgaacag cgccgacacg gtcgtcatca tgatgggacg gaaccgcacc
    20221 aggcacgcct cgtagatggc gtcgcgcgac gacaaatgct gttcacgctc ggccagcaag
    20281 gcgaagtcca ccatcatgat ggcgttcttc ttcacgatgc caatcagcag aaagacgccg
    20341 atcaatgcga tcagcgtgaa ttcctggccc agcaatatca acgccagcaa ggcgccgata
    20401 cccgctgacg gcagagtgga aagaatggtg atgggatgga cgtagctctc gtagagcacg
    20461 cccagcacga tgtacatggt gaccagcgcc gccagaatca accagggctg ctgcgacagc
    20521 acttgctgca attggccggc ctcacccagg aagcccgcct ggatctcccg ggtgggcagg
    20581 ccgatgcgcg cgacggcctg gtctatggcc tgcgtggcct gttgcagcgt gaaaccgggc
    20641 gccagactga aagacaccga atccgaggcg aacaacccct ggtgctgcac actgagcggt
    20701 gcgttctcca ccacgaaatg cgtgaacgcg gacagcggaa tgcgattgcc ggcggcggtg
    20761 accacctgca cctgctccag ggccctggca tcctgcgcct ggctggggtc cacgcccatg
    20821 accacatgat attgattgag ggcaccgtag atcaccgata cttggcgctg gctgaacgaa
    20881 tcgttcaacg tcgacgagat gagcgacatg ttcacgccca atcgactggc cgcgttgcga
    20941 tccaccacca actcgatctg gcgccccttg tcctgaacgt cggtgtccac gtcggccaat
    21001 tccggcaacg cggccagggc tttctgcacc tggggcatcc actgccgcaa caaagccagg
    21061 tcgctggcca tcaaggtgta gttgtaggcc gccgtgctgg attcatgccg gcccacgcgg
    21121 atgtcctgct gtggcaccag tgtcaaccgc gccccgggga catccaggaa tttcttccgc
    21181 aagcggttga ccacctgatc agccgaggcg gtgcgctcgg aaaacggttt gagctgaatg
    21241 agcagaaagc tggaattgct gccgccgcgc ccgccggcga accctgtgac ggtctgcacg
    21301 gcgggatcct ggcgtatggt tttctcgaag tcgtcgagct tcggaatcat ggccgtgaac
    21361 gacgtgccct ggtccgcccg gaaaaacccc aggagttggc cagtatcctg ctgggggaaa
    21421 aaccctttgg gcaccacggt gtacaggtag aagttcagcc ctatcgtggc ggccagcaac
    21481 agcatcatga atcggctgtg cgccagggcc cagcccagcg atcgcttgta cccgttccag
    21541 agccacgcac cgatcgcctg gatcagacgc gccacccgac cgggacgggt ctcggcgtcg
    21601 gccgctggcg tatgcttgag cacatgcgcg cacatcatcg gcgtcagggt caacgacacc
    21661 accagcgaca ccatgatggc ggcggacagc gtcaccgcga actcgcggaa cagccgcccc
    21721 acgatacccc ccatcagcag gatgggaatg aacaccgcca ccagcgacag actcatggcc
    21781 acgacggtga accccacttc gcgcagcccg cgcagcgcgg cgcgcaacgg gggcacgcca
    21841 cgctccatgt ggcggatgat gttctcgagc accacaatgg cgtcatccac cacaaacccg
    21901 gccgcgacga tcagagccat gagagaaatc gtgttcaacg tgaacccgaa caactgcatg
    21961 agactgaaag tggcgatcag ggacactggc acggccacgc tgggaatgag cgcggcgcgc
    22021 gcgttgcgca ggaacatgag cacaaccagc accaccagca gcacggcaat gaccagcgtg
    22081 agctcggcct cgtgcaggga cgcccggatg ctgggggtac ggtcctgtgc cacggtcagc
    22141 gagacatccg ccggcatcag ggcttcgagt gacgggagtt cggcgcgtat gccatcgacg
    22201 gtctcgatga tattggcgtc ggactgccgc cgcacgatca gcagaatggc tttcttgtcg
    22261 ttgaaaaacc cggtctggta caggtcctcg accgagtcct gaaccgtggc cacatcgccc
    22321 agccgtatcg gcgcgccatt gcgccaggcc acgatcaatg gccgatattg tgccgctttg
    22381 gtcagttgcg accccgagct cagcacccaa tggtattgat cattctcgac catgccgtcg
    22441 gggcgcatgg tgttggcatt ggccagcgcc gtgcgcacgt catcgagcgc cacgccggcg
    22501 ttgacgagcg cctgcgggtt caggttgacg cgcacggcgg gcagggaact ccccccgacg
    22561 ctgacttcac ccacgcccga cacctgcgac aacttctgcg ccaacacggt ggacgctagg
    22621 tcatacaggc tgccctggct gaccgtgttg gaggtcagcg ccagcaccat gatgggtgcc
    22681 gcggccggat tcaccttgtg ataggttggc ggggtgcgca ggctggaagg cagcaggcta
    22741 cgcgccgcgt tgatggcggc ctgcacgtcg cgcgccgcgc tgttgatgtt gcggctcagg
    22801 tcgaattgca gcgtgatgcg ggtggagcct tcagtactca ccgacgacat gtcggtcacc
    22861 ccggcgatgg tgcccagtga ttgctccagt ggcgtggcca cgctggacgc catggtttcg
    22921 gggctggcgc caggcaggtt ggccgatacc gagatgatgg ggaaatccac ttccggcagg
    22981 ggcgataccg gcaggaaccg gaacgccagg gcgcccgtca ggaccagcgt cagcgccagc
    23041 agcgtcgtcg ccaccgggcg catcacgaac agcgacagcc acctcatggc cggattccac
    23101 tcttcacgcg cgaagtgtgg cgggccgcgc gcgcgcgccg ggccaggcgg tcgaagaaca
    23161 ggtagatcac cggcgtcgtg aacaaggtga gcaactggct gcacagcaac ccgcccacca
    23221 tcaccagccc cagcggctgg cgcagttcgg accccgcacc ggtcgccagc ataaggggaa
    23281 tggcgctgaa caacgccgcc agcgtggtca tgaggatggg tcggaaccgc agcagcgccg
    23341 cctcgtggat cgcggcgcgg gccgacagcc cgcgctgacg ctgcgcctcg agggcgaagt
    23401 cgatcatcat gatggcgttc ttcttgacga tgccgatcag aagaatgatg ccgatgacgc
    23461 cgatcatgtc caactcggtg tgcgtcagca gcagcgcgag caacgccccg atcgccgccg
    23521 atggcagcgt ggacaggatg gtgatcggat gaatgtagct ttcatacaga atgcccagta
    23581 ccacatacac cgccgccacg gccgccagca gcagccacaa tgtgctgtcg agcgaagcct
    23641 ggaacgcctt ggcggcaccc tggaattcgg tctggatgga cgccggcatc gacagccccg
    23701 tctcggcttg cctgatggcg tccacagcgt cggacaacgc caccccttgc gccagattga
    23761 acgaaatcgt ggtggccggg aactgaccca ggcgttcgac ggaaagcagc gtgttcgact
    23821 gctggacatg ggccacactg gtcagtggca caggcgtgcc ggaggtcgtg gcgacgtaga
    23881 tgtgatcgag cgaggacggt gactggcgaa aaggcttggc cacctccagc acgacgcggt
    23941 actgggtgga ttgggtgaag atggtggaga tgagccgctg cccgaaagcg tcatacaagg
    24001 cgttgtcgat cgccgccgtg gtcacgccga ggcgcgacgc cgcgtcacga tcgatgacca
    24061 gcgtcgcctg cagcccgcca tcctgcagat cgtcggcgac atcggccacc tgtggcaacg
    24121 cgcgcaatcg gtctaccacc ttgggcaccc actgggcaag ctcggtacca tccggatcgg
    24181 acagcgtcat ctgatactgc gtgcgactga cccggtcatc gatgctgaga tcctgcacgg
    24241 cctgcatata gacctggatg tcgggcagct tgcgcaggct ctggcccagc cggcggatga
    24301 tctcctgggc attgtcgctg cgctcgcccg ggggtttcag cgcgatctgc atgcgaccgg
    24361 tgttcaaggt ggaatttgtg ccgtctatgc caatgaatga cgtgacagcg gcgacgtcgg
    24421 ggtctttgag gatgagatcc accgccttct gctgtcgctg cgccatcgac gagaacgaca
    24481 cagtggccgg ggcctgactg atggcctgga tgaggcccgt gtcctgttgc ggaaagaacc
    24541 cctttggcac cagcccatac agcaaccctg tcacgacaaa cgtgcccagc gcgatccata
    24601 gcgtgatgcg ctggtgcgac agcacccatt tcagaccatg gtcgtaccag ccgaccagtg
    24661 tatccatccc ccgccccagc gccacctgca cacggccgta cttctgttcg gattcggcct
    24721 tgagcaagcg cgcgcacatc atgggcgtga gcgtgagcga gatcaacaac gacatcagga
    24781 tggcgaccga caacgtgatg gcgaattccc ggaacaatcg cccgatgaca tcactcatga
    24841 acagcaacgg gatcagcacc gcgatcaggg aaaccgtcag ggacaccaag gtgaatccga
    24901 tctgcgcggc ccccttcaat gcggcgcgca tcgcggtttc accctcttcg atgtgccggg
    24961 cgatgttctc gatcatgacg atggcgtcgt ccaccacgaa gcccgtcgcc acagtaaggg
    25021 ccatgagaga cagattgttg atgctgaagc cggccagata catgatgccg aacgtcccca
    25081 ccaatgaaag cggcaccacc acgctgggca ccaatgtggc cgaccacgtg cgcaggaaca
    25141 cgaatgtcac cagcaccacc aggaagatgg ccaacagcat ttcgcgctca acgtggctga
    25201 tggagtcacg tatggtgtag gtgcgatccg tcgcgacagc gacgtccacc ccggccggca
    25261 acgccttctt gagcgatggc agcaacgcct tgacacgatc gaccacgtct atgacattgg
    25321 cacccggttg gcgctgaatg ttcatgagca ccgcgggcgt gtcgccgatc caggccgcct
    25381 ggcggatgtc ttccggattc tgccgaaccg tggccacgtt tcccagatgc aacgcggccc
    25441 cgttttcgta tttcaggatc aggttttcgt aatcgctgac ggacttgagc tggtcattcg
    25501 cattgatggt gatcgaccgc tgcggaccat caagattgcc catgggctgg ttgacattcg
    25561 cccccgtgac cgcggtgcgc acgtcctcga gcgtgaggtt gtgctgtgcc agagcctgcg
    25621 gattgacctc gatgcgcacg gccggacgct gcccgcccgc gatgctgacc aggcccacgc
    25681 caggaatctg agacagcttc tgcgccatgc ggacatcgat caggtcgcgc acctcataca
    25741 gcggcaacgt gggcgacgtg atcgccaggc tgatgaccgg cgtatctgcc ggattcacct
    25801 tgttgtaggt gggcggcatg ggcaagtcgg tgggcagcag gttggaggcc gcattgatgg
    25861 ccgcctgcac ttcctgttcc gccacatcca tggacagcgt cagatcaaac tgcagcgtga
    25921 tctgcgacga tccacctgaa ctcgtggatg tcatctggga cagcccgggc atttgcccga
    25981 actggcgctc gagcggggag gtgatcaacg cggtcatcac ctctgcgctg gcccccgggt
    26041 acagcgtgac aacctggatc gtggggtaat cgacctctgg caatgccgac accggcaaca
    26101 ggcgatacgc caacacgccg gagatcaaca acgcgatcat cagcagcgtc gtggccaccg
    26161 gcctaaggat gaacaggcgg gagaagttca ctgcgcgcta cctgtgacct gttccccgcg
    26221 acgagccact tttcgtggtg cccgccgcgc ccccttctgg gggcgccact gcccctcctg
    26281 acgcagccga ccccggcatc gccttgcccg cggcggtctt cccggctgtg gcatcgctcg
    26341 cctcgatgac cttcaccgcc gccccttcac gcagccggtc cacccccacc gtgaccactt
    26401 gctggcctgg gctcagcccg ctggaaaccg cgacatcagt accatcaacc gtgcctgtct
    26461 tgacgggctg cacatgcacc ttgccatcct tgaccaggta gacgaatgcg ccgatcgacc
    26521 cttgctgcaa ggcgctggtg ggaatcatga tggcgtggtc atcggcgctg acgtgcagca
    26581 cggcgttcac gaattcgttg gggaacagcg cttcgttttc gttggcgaac gtggcgcgca
    26641 acttgaccgt gccggtggtg acgtcgatct ggttgtcgat ggaagccagc gtgccggtgg
    26701 cgatcttgtt cacaccgcta cggtcgtata aatccacttc cagcttcttg ccagcccgga
    26761 cctcggccag gaccggcggc aattgcgcct gcgacagcga gaaaatcacg gaaatgggcc
    26821 gcatttgcgt gatgaccacc agaccttgag tgcccgacgc cgaaaccagg ttgcccggat
    26881 cgacctggcg caggccgacg acgcctgtga tgggcgcggt gatgcgggtg aaggtgagct
    26941 gcaaccgggc gctggccacg gcagccgcgt cgcttgtctg tgtgccaaga tattgctgaa
    27001 ccagcgcctt ctgggtatcg agctgttgcc cggccaacga gttctgcttg tagagcagcg
    27061 tgtagcgttt tagatcgcgc cgcgcgttct cgagcagggc ttgattctgt ttctctgtgc
    27121 cgatggcctg gtccagcgcg acctgatagg gacgtgggtc gatctgcgca agcagagcac
    27181 ctttttttac ttcctgcccc tctttgaaat agacgccctc cagttcacca ctgacacggc
    27241 tctgtaccgt gacggcattg agggccgtga ctgtgccgat ggccttgatc gtggtctcta
    27301 taaggccttg cttgacggtg gccacgttga ccggcgtggc catggcgccc gccatgccgc
    27361 ggcccccgtg ccccgaacca ttacccgcag ttgcacctgg tttgtgcgaa aaatgccaat
    27421 acgcgccaaa ggcaatgacc aggatgaata acgccaccca ggacaagccc tggcgtggcg
    27481 acttaggctt ggcgctcggc tgcgacatgc gcggctcccg aatcgacgaa caatgtggat
    27541 acaaccagtg gaaggactac attgtcgccc aactgcgcaa cgtcgcgccc cgatacgcgc
    27601 atatttgaaa tcaaacgcaa caactacccg ttccggatcg attgaagggt gcggccgcga
    27661 cgccgcactg tattacaggc gccgcgtcag acgttgcagc gtgaaagatg aaagggaatg
    27721 gtgcgattcg ccggttgggg tttgagctct tggtcggccg tggagaaccg gacccatatg
    27781 acgtatttat tggcgctcat ttcgggaaac acctgcagcg acgggtcgac ccagacacgc
    27841 aggagctgga atgtcttgcc gcccagcatt tcctggtagg cgccctgctc ggcttccagc
    27901 ggagtgggtg tgctggaatc gcgcaacaga cgcaaaatga gcgccaaccc ctgatgcaat
    27961 gacagaaagg gctctatcca ttgccgcaag tcgttgcaac gcgactcgcg cggcttgatt
    28021 tgccaagcat aataagaggg catgtcgacg ggcgaggagc cgccaggcac cgccacacgc
    28081 ccccgcaggc tggccagcca ttcgttgtcg cgaagcacct ggccgatgcg gccctgcgac
    28141 cccaggttgc cggaaacctc ctggatttcg gcaagcatgg tatccagtgt gtcgacatcg
    28201 acgccgggat gctggcgtag cccgcccaat gtgacgcgtt ggcgttcgag gtcttgcagc
    28261 accgcgccgc gcaagtcggt gcggtcgctg atgtcgagca ggtcgaacaa ggtggcgacg
    28321 gccacctgat ggtgccgcac atcgtcgccc tcgacgaaat agaacagccg gtcgaacaga
    28381 tactcgaccc gcaacaaggc cctgacacgt tcattgcagg ggtattcgta gagaatcacg
    28441 cggcaaaaaa ccttttattg aatgagtgcg ccatcaagat agagcctgcc ggtgcgccgc
    28501 ctgaaggcgc catgccgcat gcaggagccg aacgcgctgc gcaagatcgt cgagcgacgt
    28561 gccttcccca ttgaacacga catcatcggc tgcggccaag cgcgcctcgc gcgaagcctg
    28621 cactgacata atacgcgcaa tagcctctgg agtcagccca ctgcggaccc gcacgcgggc
    28681 gatctgtgtc ggctcgtcac aatcgaccac gcagacccga tcgacccgat cgcgccaacg
    28741 ccccgactcg accagcaacg gcaccacgta aaccagatag cacccttgcg cccgcgccgc
    28801 ttcggcttgc gtgacggacg tgatcatggg gtggagaatc gcctcgagcc gttggcgcac
    28861 cgacatgtcg ctgaaagcga gttcgcgcat gacggatcga tcgagcgcgc cgtcggccgt
    28921 gatgatccgg ccgccgaatt cgcgacgcag gggttcaatg gccgccccac ccgacgccgt
    28981 cagggaatgc gcaatggcat cggtgtcgat gatggccgcc cccccgtcgt ggagcatgtc
    29041 agccacccgg ctcttgcccg aaccgattcc gcccgtcaga cctattgaat acatgcccat
    29101 tccgaatctt gtcgatgtgt ttcgccacgc gatgcggacc gccatgcccc ggccagcaca
    29161 caggcggtgc gaggccgccg tgacctcaac ccgataacgc gcattgtgcc agcataaagc
    29221 cggccccggc cagcgccagg aatggaccga agggatacgc acccgccggc cgccaggttt
    29281 tctggcgcac acacgcaaac aagaggccgc caaggcacgc ggccagcaac gcgagcaggg
    29341 cgggatgcgg ccccagccag gcacccagcg ccgccagcag cttgaagtcg ccgtacccca
    29401 tgccgtcacg cccccgaacc gcccgaaacg cctgaaacag cagccagagc agcccgtagc
    29461 cgaccatggc gcccgccacc gcgtcgtcca acgacaccgg cccgcccagc caggcgatgg
    29521 cgagccccag ccaaagcaat ggcagcgtca gcgcatcggg caatagcgat gtccgggcat
    29581 cgatgagggc cagcgccaac aacatgcccg ccgccacgaa cgcgccaaag aacaccggcg
    29641 tccaaccgtg gcgccagtaa aacacacccg ccacggccgc atagaacagc acacccaccc
    29701 cgcacaacat ccggagcggc acacgcgccc gggacgccga gtggcgcgcg cctccgaaag
    29761 acctttgaat acggcgtccc gcccacacgg cgcccgccaa caccagacac aggcccatcc
    29821 aatccgccac ggacccggcg gatcgcgcgc cgtgccatgc cgggctgaaa aggtcaggcc
    29881 acatgcatcc cccttgtcag acaccctcct cccgcggacc atcgccgatc aaacgtcgct
    29941 tttcggcttc ccgtcgacag cggacgcggc gataatacac acaaccgcca atgccataca
    30001 cggaatcccc acatgcccca cctcattcaa cgttttcttc gccccgtgct ccacgcgagc
    30061 ctggccgcgg ctttcctgac actcgccggc tgcgcccagc aacgcgcgcc cggctactac
    30121 gccgtgcaac acgacaacac atacagtgac gcccagcaac agggacaagg caccgcgcaa
    30181 tccaaggccc cctcgcaatt gcaactcgga ttcggcagcc agaatcagaa agcgaacgac
    30241 aacccggcgg ggaacaccga atcagccgtc cagacgcgcg aatgggaagc cacccgcccc
    30301 ttgcgcgaac ccaagacgtt cctgggcacg gtgccttgcc tgacctcgcc gggcgcgtgc
    30361 gcggcgaccc gcgtcaccct cacactggcg ccatcgggcg aatggcgcgc ccgcagcacg
    30421 ccattgggcg gcaacggctc gaacacaggc ggcgcggtcg cgcagcaggg ctgctggcac
    30481 gtcattggca ccgcccccct gaagatactg ctgcagttga acaacctgaa tcccaaggcc
    30541 acgctcacct tcatcaacga caacgtgctg cgcatcgact cgttcaacgg cgtcacgccc
    30601 aatctggaat atcggctgac tcgccaggcc gacatcgacg gcatcgacga actgcacggc
    30661 aaacccgtgt tggactgcaa gacgaaataa agtccctacc cggccggcag atgctgcgac
    30721 atgacgcaac gggtaagctc gacagcgttc ctgacacgca gcttctggaa caccctggcg
    30781 cgatgcgcct cgacggtgcg ctgagacacc cctagttcgg cggcgatgat cttgttgggc
    30841 ttgcccagcg ccacataacc catgacatcg cgctcgcggg gggtgagtgt cgaaaagaac
    30901 gggtggtcgg tgtcgaatcc ccctgcggcc atgatggtct cctgtctatt tgttagtaat
    30961 aagacggatt ttgtcatgac aagttacacg tgtcgcctgt catttctgac agatcggtgg
    31021 ctcaaaacaa cacgcgtaac cgatggggcc tcatatttcc agattgtcga tgagccgggt
    31081 ggcccccagt ttggcggcgg ccaacacgac cagaggctct ccggcctcga tttccccggc
    31141 ttgcggcggg ttcaaatcac gttgacggcg cagcgacaca taatccaccc gccagtcgtg
    31201 cgcggcaagc tcgtcgcacg cccgggcctc cagcgccgca ccgtccgtgg caccgccacg
    31261 cacctggtcg cgcagatcct gcagaacgcg atagagcctg ggcgcctcgg cgcgttcggc
    31321 ggcttccagg aagcgattgc gcgaagacag cgccagcccg tcggtgtcgc gcacggtctc
    31381 gtgggccaga atatccacgg gcaactgcaa ctggcgacac atgctgcgca cgaccatgag
    31441 ttgctgataa tctttcttgc cgaagaccgc gacacgcggt tgcacgcagg cgaagagctt
    31501 ggtgaccacg gtgcacacgc ccgtgaagaa cccgggccgg aattcgccct ccaggatgcc
    31561 tcccaggtcg tccggcggca gtaccttgta attctggggc tcggggtaca tctcgtgctc
    31621 gtcgggcgcg aagagcacat agacatcgcg ctcgcgttcc agtttttcga tatcggcctg
    31681 cagggtgcgg ggataacgat cgaaatcctc gctcggaccg aactgcagcc gattgacgaa
    31741 aatgctggcc accaccgggt caccgtgctg acgcgccaat ttcatcaggg aaagatgcgc
    31801 ggcatgcaga ttccccatgg tgggaacgaa cgacacacgc tgctggccgc gcaactggtc
    31861 gcgcagttcc tcgatggtat gaacgacttt caagagcttc tccgtgtggg ggcgggaccg
    31921 gcgcccggga agtcaggcac tgggaacata cgacaggcgc acgtagatgg gtgcgtaagg
    31981 ttcggcctgc gtgatctcga gcaacgattc ccgcgccaat tccagcatgg ccaggaaatg
    32041 caccacgacg acggcggcgg gatcgccctc gcgcaccccc tccatgaaca gctcggagaa
    32101 ctccaggaac cgcacgtcgc ccaaccgacg caggatgcgg ctcatgtgat cgcgcacgga
    32161 aagctgctcg cgggtgacac ggtggctggc gttcaggctg gcgcgcttca tgatgtcgac
    32221 ccaggcttgg cgcagatcat cggggctgac ctcgggcagc atgcgttcga cctgcaaatc
    32281 gggcagcgca tgggaacgct ggaaatcgcg ccctgcccgc ggcagggcat cgagcttgcg
    32341 ggccgccaat ttcatctgct cgtattcgag caggcggcgc acgagttccg cgcgcggatc
    32401 gtcgggctct tcgcctgtgt cggacttggt gatcggcagc agcatgcgcg acttgatgtc
    32461 gatcagcaac gccgccatca acagatattc gcccgccagt tcgagattct ggctgcggat
    32521 ctgctcaacg taggacagat actggcgcgt gacttccgcc atgggaatgt ccaggacatt
    32581 gaagttctgc ttgcggatca ggtagagcag cagatccagc gggccttcga acgcctcgag
    32641 gaagacttcg agcgcatcgg gcgggatgta gagatcttgc gggatggcga acaacggctc
    32701 gccgtacagg cgcgccaggg cgacgctgtc gacgatatcg ggcgtgctgt cgacagccgg
    32761 gtccaccagc gcgttcaggt cggccatatc ggccccgggg gaggacacgg gaggcttccg
    32821 ggcgtcattc catgtcgtaa tacacgtacg ggcgctgcgc cacgcgggcc gagcggaagc
    32881 catccagcgc ctcggaatcg agctgccggt cccacagacg cgcacgcccg gcgcgttgcc
    32941 gcgcatcggt tcccgggttc tctgtcttgt acttcttcag gaactgggtg atttccgact
    33001 cgtaattctt tgccatgatg cataatcgtc gttaatgtcc gaaatgcgag tttactgcac
    33061 tcgagccgtc tgtttcaccc atgcccgcca acccgctccc cgccccaccc acctcccttt
    33121 ccccgcgcga gcggtccgcc atacggatgc ttccgttcat tgtgggctgc gcgctgttca
    33181 tgcagatgct cgattccacg gtcgtggcca ccgcactgcc cgtcatggcg accgccctgg
    33241 gctcgacacc ggtccagatg aacgtggtga tcaccagtta tctgctggcg gtcgccgtgt
    33301 tcgttccgat cagcggctgg gccgccgacc gattcggttc gaagcgggtg ttcatcgcgg
    33361 ccatcctgct cttcacgctc agctccattg gttgcgcagc ctcccagaat ctggtcgaac
    33421 tcgtggtggc gcgggtcttc cagggcatgg ctggcgccat gatggtcccg gtagggcgca
    33481 tcattctgct tcgccgggta cccaagagcg aactcctcaa ggcgatgtca ttcctgtcgc
    33541 tgcccgccct gctgggccct atcatcgggc caccgctggg cggtttcctg gtgacttacg
    33601 cgtcgtggca ttggatcttc ctcatcaaca tcccggtcgg catcctgggc atcggcctgg
    33661 tcacgtatta cgtgcgcgat gattaccccg ccggagcccc gcggttggac tgggtcggtt
    33721 tcattctcag cggcatcagc ctggcgacgc tggtttcagg gtttgaatcg ctgggccatc
    33781 aagcgggcgg ggcgttgcag agcctggtgc tgatcggcac cggtctggcc tgtggcgcgt
    33841 tgtactgctg gcatgcccgg cacacggcac accccatcat cgacctgtcc ctgctgcgcg
    33901 tccgcacctt cgcggttgcc accctgggtg gcaacctgtg ccgcttcgcg gtgggcgcca
    33961 gtccgttcct gctcgccatc ctgctgcagg tggggttcgg tctgtcgccg ttttcagccg
    34021 gcatgatcac gttcacgggc gcggcggggg cgattctcat gaaactggtc gccacaccga
    34081 tcattcagtt ctttggcttc cgccggatcc tcacgatcaa tgccctgatc gccgcggtgt
    34141 tcatctccct atgcggtctg ttccggtccg atacgccggt gtggatcatg gtggtcatcc
    34201 tggtgagcgg cggattcttt cgctcgctgc agttcaccgc ggtcaacacc ctcacctacg
    34261 ccgacctggg ttcccaggaa atgagccgcg ccagcagttt cgccgcgatg gcgcagcaac
    34321 tgggcatcag tttgggcgtg gggtgcgcgg cggcggtgct caatctcagc atgcgctggc
    34381 gaggcgacac ccatctggcg ctgcccgaca tcatcgcggg gttcctgttc atcggggcgg
    34441 tcacagcggc atcgtacttg tcgttccggc gtctcgagcc cggcgccggc gaccacatac
    34501 acaaaaacag gaaagtggcc agggcgtcgc ccacaacctg acacgcgacg ccctcgcttg
    34561 gcaccgacgc gggcgggccg tgacggtgcc ggcacggcag acccacgaca acccgacacg
    34621 cttcacgcct cgggcatgag catcacaagc ttgcagtctg ggcgaatcgc catgcggaag
    34681 gtgatctgcc gaaaggccag cagcccccgg gcggggttgt tgaactcgcg cagcccgcct
    34741 tcgcgctcga ccacggtctg ccgtgtccac cagtgcgcga actcccgact gcccgcctgc
    34801 agttcatcga gcacctcgcg cacgccggga tcgtcggcat gcgggcccgc atccgcgcgg
    34861 aactcggcca ccacccgccg cgcccgctgg tcccaatcga cgatcaacga gcgtgcggcg
    34921 gggtcgagaa agatgtagcg caacagattg gggcgatcgc tgcggtccgg ccacccgtcg
    34981 aacagcgcca gcagtgcgtc attgcgcgcc agcacattcc aacaacgatc caggatgtac
    35041 gccggggcgg caatgctgtg gacgcagccc gccagatccc cgggcagcgg ctcggggttc
    35101 atgccaccgt gctcggggtc ggcgcattcc gccagatcaa acagatagtg ccgctcggcc
    35161 ctggccaggc gcagcactgc ggccaggcgt gtccagacac ccggcgacac agtcacacca
    35221 cgcccttgtt ctatccatgt gtaccaggtg acgctgatgt cgcagagttg cgccacctcc
    35281 tcacgccgca ggccgggtgt gcgtcgccgc acacccgcgg gcaacccggc catctcgggg
    35341 gtgacgcgcg cccgggcact gcgtagaaaa tcgcccaggg cgcggcggcg cgcctgcccc
    35401 gcctgcgctt ccggcacacg caagggtggc atcgggctct gctcgccttg ctccgtccgg
    35461 atcatcgcaa cgcgctggcc agagtgcggc ggacatcatc ctcggtgcgg ccgctgcggc
    35521 gcatgtagtc acgcacctga tcgtcgccaa tgttgcccag gttgaagtac tgcgacatgg
    35581 gatggctgag atagaacccc gacacactgg aggccgggta catggcatag ctgtcggtga
    35641 gcatcatgtc gatatcgtcg cactccagca ccctgaacat atccttcttc acgacatgct
    35701 ccgggcaggc cggataaccg ggtgccggcc ggatgccctg gtatttctcg gcgatgatgt
    35761 catcgttgcc gagattctca tctggcacat atccccagag atcgcgccgc acccgcgcgt
    35821 gcaggcattc ggcgaaccct tcggccaacc ggtcggccac ggccttgagc atgatgctgg
    35881 aataatcatc gttgtcagcc aggaagcgtt catcgtgctt ttctatgccg atgccgcccg
    35941 tgacggcgaa caggccgatg tagtcggcca cacccgttgc cttcggcgcg atgtaatcgg
    36001 ccaggcattt gttgtcgacc ccttcgcgct tttccgtttg ttggcgcagg ttgcgccagg
    36061 tgagcaagac ctcggagcgc gattcatcgc gatagacctc gatgtcttcg tcattgaccg
    36121 agttcgcggg gtaaaacccc accacgccgt tggccgtaag ccagcgccca tcgatgacct
    36181 tgcgcagcat gtcgcgcccc tcggcgaaga tcttgcgcgc ctgctcaccc accaccttgt
    36241 catcaaggat ggccgggtac tggccaaaga ggctccaggt ctggaagaac ggcgtccaat
    36301 caaggaattt ctccacctcg gtcaggtcgt aatgcttgaa cgtgcgccgg cccaggaatt
    36361 tggggcgcgg cggggtgtag gccgaccagt ctatgaccgg ccgggccgca cgtgccttct
    36421 cgaggctgat aagcggtgtg gtcttgcggc tggcatggcg tttccgcacc agttcgtact
    36481 cttcggcgac ctccgccaaa taggtgccgg cctggtcgga catgagactg gtcgccacgc
    36541 ccaccgagcg actggcgtcc ggcacgtaga tgaccggccc ttcgtagttg ggcgcgatct
    36601 ttaccgccgt gtgcacacgg ctggtggtcg ccccccccac cagcaacggc agcttgcggc
    36661 tgaggaaata gtcgtcccgc tgcatttcag acgcgacata agccatttcc tcgaggctgg
    36721 gcgtgatcag acccgacagg ccgacgatat cgcagttctc tgccttggcc ttggcgagga
    36781 tctccgcaca gggcaccatc acgcccatgt tgatgacttc gaagttattg cactgcagga
    36841 ccaccgtgac gatgttcttg ccgatatcgt gcacgtcacc cttgaccgtg gcgatcacga
    36901 tcttgccctt ggagcgcacg tcgccaccag cctcggcgat ctggcgtttt tcttcctcga
    36961 tgaacggaat gagatgcgcc accgcctgct tcatgacgcg cgccgacttg actacctggg
    37021 gcaggaacat cttgcccgcg ccgaacaggt cgcccaccac gttcatgcca tccatgagcg
    37081 ggccttcgat gacctcgatg ggccgcccgc cccgcgcggc gatagcctgc cggacttctt
    37141 ccgtatcttc gacgatgaat gacgtgatgc catgcaccag cgcgtgcgtc aggcgttcct
    37201 ggaccgggcc ggcgcgccag gccaggtctt cctctttgcg ggcgccacca cccttgatgg
    37261 tctcggccaa ctccaccagg cgctcggtgg acgtgcgctc atcggcggga tcggtccggc
    37321 ccaccggttc gacacggtcc agcaccacgt cttcgaccat gtcgcgcaag gtcttgtcga
    37381 tgtcctcata gaccccgagc tggccggcgt tgacgatgcc catggtcatg ccattcttga
    37441 tggcatggta gaggaacacc gtatggatgg cctcgcgcat ggcctcgttg ccacggaacg
    37501 agaagctgac gttcgacacg ccgcccgaca cccgcgcgtg cggcaggttc ttcgtgatcc
    37561 agcccgtggc ttcgatgaag tccacagcgt aatgattgtg ttcgtctata ccggtggcga
    37621 tggcgaagac gttgggatcg aagatgatgt cttcgggcgg gaacccgacc tcatcgacca
    37681 gcagatggta ggccttggaa caaatgtcta tgcgccgctg cagcgagtcg gcctggcctt
    37741 tttcgtcgaa cgccatgacc accaccgccg cgccatagcg gcgacacagg cgcgcctggt
    37801 g
//
LOCUS       DASTNK010000035        49018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1320797, whole genome shotgun sequence.
ACCESSION   DASTNK010000035 DASTNK010000000
VERSION     DASTNK010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 49018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49018
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320797"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2442)
                     /locus_tag="VFR20_02840"
     CDS             complement(<1..2442)
                     /locus_tag="VFR20_02840"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU0229484.1"
                     /translation="MTPTSSQNDASQPPKSFEPQEIESRWYAQWEQRGYFKGGQHVAP
                     AAGTESHPFVVQFPPPNVTGTLHMGHAFNQTVMDGLVRYHRMRGDDTVFIPGTDHAGI
                     ATQIVVERQLDAQQVSRHDLGREKFTEKVWEWKQKSGSAITEQFRRLGSSADWSREYF
                     TMDANLSAGVAETFVRLYEQGLIYRGKRLVNWDPVLGTAVSDLEVVSEEEDGHMWEIR
                     YPLVEPVGALTHLVVATTRPETMLGDVAVMVHPEDERYAHLIGRRVRLPLTDRDIPVI
                     ADAYVDREFGTGVVKVTPAHDFNDYAVGQRHGLEMISILTLDAHISDAAPAAYHGLDR
                     FEARKRVVDDLAALGLLRQVRPHKLMVPRGDRTGTVIEPMLTDQWFVAMSKPAPEGTL
                     HPGKSITQVALDVVADGRIRFYPENWTTTYNQWLNNIQDWCISRQLWWGHQIPAWYAP
                     DGRIFVARDADEARAKAAAAGVTEPLTRDEDVLDTWFSSALVPFTDLGWPRDTPDLER
                     YLPSSVLVTGFDIIFFWVARMVMMSTHLTGHVPFRAVYVHGLVCDMDGKKMSKSKGNT
                     IDPVDLIDGISLEALLDKRTTGLMNPKQAAAIRKKTGKDYPQGFPAYGTDALRFTMAA
                     YATLGRNINFDLKRCEGYRNFCNKLWNATRFVLMNTSGHDLTGGDRGTLSFVDHWIIS
                     ALQRLEEEVQRGFTEYRFDNIANALYHFIWDEYCDWYVELAKVQIQHGTPQEQQGTRR
                     TLIRVLETLLRLAHPIIPFITEELWQKVAVDAGKRGADEETSISVQPYPVVNPALIDA
                     EAEARVAELKAQVEAI"
     gene            2717..3142
                     /gene="ndk"
                     /locus_tag="VFR20_02845"
     CDS             2717..3142
                     /gene="ndk"
                     /locus_tag="VFR20_02845"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU0229485.1"
                     /translation="MAIERTLSIIKPDAVAKNVIGQIVARFEQAGLKVIAARLQQLSR
                     SDAERFYAVHKERPFYRDLVDFMVSGPVFVQVLEGDNAVQKNRDLMGATDPKKAAPGT
                     IRADFADSIDANAVHGSDAAETAAVEVAFFFPEYAILSR"
     gene            3194..4345
                     /gene="rlmN"
                     /locus_tag="VFR20_02850"
     CDS             3194..4345
                     /gene="rlmN"
                     /locus_tag="VFR20_02850"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU0229486.1"
                     /translation="MPTVVEPINLLGLDSAALTDLVGQWGSKPFRARQLERWIHQRSA
                     DSFDQMTDLARDLRQQLSQRCLIRAPRVLTEHLSADGTRKWLFDVGRNNAVEAVFIPE
                     DDRGTLCISSQAGCTVACPFCSTGYQGFNRNLTTAEIIGQLWHAKRAVEADPARARTS
                     HADDAPMRAISNVVMMGMGEPLLNYGQVLGALRLMLDDNAYGLSRRRVTVSTSGVVPM
                     MDRLAADCPVALAVSLHAPNDALRDRLVPLNRKYPLADLLAACNRYLEHAPRDFITFE
                     YIMLDGVNDTDDLARELVGISRQVRCKFNLIPFNPFPQSGLKRSPPARVRLFAQRLMD
                     AGVVTTVRKTRGDDIAAACGQLAGDVKDRTRIDERLPDRAVIARNQECG"
     gene            4342..4872
                     /locus_tag="VFR20_02855"
     CDS             4342..4872
                     /locus_tag="VFR20_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0229487.1"
                     /translation="MTPPSANRASLDDDPVLSRPDAGTDIYQGPQGLGPTLRALREAK
                     RISLAEVSGRLKFSVRQFEALESEKWDLLPTGVSLRGFVKNYGRFLEADIDALITMLE
                     NQVGGSRTRPTAVVDAPSLASGGDVPMHPDATTHSWGWLVIIVLILLVAGFYAIERGW
                     IPNSWLVFDWLKSLKK"
     gene            4869..6185
                     /gene="ispG"
                     /locus_tag="VFR20_02860"
                     /gene_synonym="gcpE"
     CDS             4869..6185
                     /gene="ispG"
                     /locus_tag="VFR20_02860"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEU0229488.1"
                     /translation="MNSIPDSITDADVPSPVGAAARRKTRPVVVRWGRHAVRIGGDAP
                     VLVQSMTNTDTADATATANQVRDLARAGSELVRITVNTPEAARQVAAIREKLDRMGVA
                     VPLVGDFHYNGHRLLSDFPDCAQALSKYRINPGNMGGGKKRGDNFAQMIEVACRHDKP
                     VRIGVNWGSLDQELLARMMDDNNRRAQPWDARAVMRRALVVSAVSNARRAEELGLSPN
                     GIILSCKVSHVQDLITVYQDLSAQCDYPLHLGLTEAGMGSKGIVASTAGLAVLLQQGI
                     GDTIRISLTPEPGGDRTREVVVAQEILQSLNLRAFTPMVVACPGCGRTSSTVFQELAA
                     SIQGYLREQMPVWKTQYPGVETMNVAVMGCVVNGPGESRHADIGISLPGTGEVPSAPV
                     YVDGQRVVTLKGDSIAEEFEAIVQRYVERRYGGGDRDGGPADETTH"
     gene            6271..7560
                     /gene="hisS"
                     /locus_tag="VFR20_02865"
     CDS             6271..7560
                     /gene="hisS"
                     /locus_tag="VFR20_02865"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU0229489.1"
                     /translation="MTQSFQKVRALTGMKDVLPDTSAQWESLESTVHEWLGAYGYRNM
                     RTPVLEQTRLFARGIGEVTDIVEKEMYSFTDSLNGEQLTMRPEFTAGMVRATIEHNIL
                     YDRPQRVYAMGPVFRHERPQRGRYRQFHQIDVEALGFAGPDIDAELVVMLARLWRKLG
                     LTDVRLELNSLGQADERAAHRAALVAYLERHQSVLDDEARRRMYTNPLRILDTKNPAM
                     QGLADNAPRLSDFLGAASRGHFDGVRERLDEAGIAYRLNPRLVRGLDYYNLTVFEWVT
                     DMLGTQGTVCGGGRYDGLIELLGGKPAPAVGFAIGMERLLELCEQVAAPLDRSECQVY
                     VVHQSDAAQRTAALAAERLRDAGLRVIVHAGSSGFKSQFKRADASGAAAAVIFGDEEL
                     SSGTASIKWLRSDTTPSQAQQESVPIERLVDELKQRV"
     gene            7564..8199
                     /locus_tag="VFR20_02870"
     CDS             7564..8199
                     /locus_tag="VFR20_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0229490.1"
                     /translation="MAYDLEEQEKIDALRAWWDKYGTLCSVVLFAAVLAVLGWRGWQW
                     YQGHEANQAMGYFEALETAAGQPGDDAVARIKAASKTLRSDYASTGYTPRGVLVAAEA
                     LQRRNDLAGAREQLEWLIHHGKDSALAALARLRLAGILLQQKQYDQALTQVQNAPTAF
                     AGLYADRRGDILAAEGKKDEARRAWQAAIKALGSDPSTQVIELKIDALTGA"
     gene            8202..9359
                     /gene="bamB"
                     /locus_tag="VFR20_02875"
     CDS             8202..9359
                     /gene="bamB"
                     /locus_tag="VFR20_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HEU0229491.1"
                     /translation="MRLPSTRRALRVVLLAIGAAALAGCSLFSSPDPRFEPAPLTQYT
                     AGVALRQIWSVSLGSGSGYGFVPVVVGDSVYAAAPSGKVSKIDLATGHVQWQASTKKT
                     LTAGVGSDGTITAVAEIDGSVVAFNDQGHEAWHAQASSEVNIPPTVGDGVVVVRGSDY
                     RIQAFDETTGKPLWNVQRPGPALALKTNMQMILVQGMLISGLPNGRLIAINAHTGSVQ
                     WEGPVSISRGATDLERISDVVGTPQIQGPALCGVTYQGHVSCFDVSQGGRPMWSKAFS
                     STTGMASDAQQVYLSNQSGVVYAFDLSDGHQAWKQSALRNRRLSEPAVVRQGVAVGDL
                     AGYVHILSRTDGHLLGRIQVGSDPIVSPLVATTHGLLVQSGSGNLVLVGVN"
     gene            9356..10759
                     /gene="der"
                     /locus_tag="VFR20_02880"
     CDS             9356..10759
                     /gene="der"
                     /locus_tag="VFR20_02880"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU0229492.1"
                     /translation="MTHTELKPVVALVGRPNVGKSTLFNRLTRSRAALVANQPGLTRD
                     RHYGEGRVGDRPYIVVDTGGFEPIAKDGILLEMARQTEQAIVESDVVIFLVDARAGIN
                     AHDHEIASMLRRSGQRIFLAVNKAEGMRSGIATAEFHELGLGEPHAISASHGDGVVDL
                     IEQALGSLPGAEDVSEVNAVVDGPEGEGPGDSTDDSVRHRIKLAIVGRPNVGKSTLIN
                     TLLGEERVIAFDLPGTTRDAIEIEFERAGVAYTLIDTAGLRRRGKVFEAVEKFSVIKT
                     LQAIEACNVVVLMVDARAEISDQDAHIAGFIVESGRALVVAINKWDGLDAEERERVRR
                     DFDRKLHFLSFAKHHTISALKAKGINDLIRSVGVAHAAAFLKMPTPKLTRTLQAAVEQ
                     QPPPRKGIFRPKMRYAHQGGQNPPLIIIHGNALDAISDSYKRYLESRFRQVFKLDGTP
                     LRIEFKSTRNPYVQESA"
     gene            10756..11844
                     /gene="hisC"
                     /locus_tag="VFR20_02885"
     CDS             10756..11844
                     /gene="hisC"
                     /locus_tag="VFR20_02885"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEU0229493.1"
                     /translation="MNQFWSDQVAGLTPYTPGEQPKGQPIVDLLKLNTNENPYGPSPR
                     VIEAIQAAVGDRLKLYPDYSAAALRAAIADLHGLAPEQVFLGNGSDEVLAHVFDALFR
                     KPGRALVMPDISYSFYRTYCRLYDIEPELIPLAEDFSIRVDDYLRPRATVPAGIIFAN
                     PNAPTGIALSVDDIERILLANPDCPVAVDEAYVDFGAESAIPLLARHPNLVVIHTLSK
                     SRSLAGLRVGFALAGAPIIEALTRVKDSFNSYPLDTLAQAGAVAAIQDTAYFDATREK
                     VIAARDDLARRLSGMGFEVLPSRTNFIFARHPAHAAQDISQALRDKHILVRHFRLPRI
                     EQFLRITVGTPGDCDRLCRVLAAFLGTP"
     gene            12007..12243
                     /gene="hfq"
                     /locus_tag="VFR20_02890"
     CDS             12007..12243
                     /gene="hfq"
                     /locus_tag="VFR20_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEU0229494.1"
                     /translation="MSNKGQTLQDPFLNALRKDHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTVTQMVYKHAISTVVPARPVNFQADDSAE"
     gene            12353..13453
                     /gene="hflX"
                     /locus_tag="VFR20_02895"
     CDS             12353..13453
                     /gene="hflX"
                     /locus_tag="VFR20_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU0229495.1"
                     /translation="MKVLIISVDLGSPDFQAHSEEFAMLARGSGAEVAHCLSVRRQRP
                     DATYFIGSGKLQEAILLADDAQVDAVLFDQPLTPAQQRNLEREFKRAVIDRVGLILDI
                     FALRAKSREGKLQVELARLQYLSSRLTRMWSHLERQRGGIGARGGPGESQLEMDRRII
                     IDKVRGLRTQLERVQRQRVTQRRARARGTTLSVSLVGYTNAGKSTLFNALTRAGAYAA
                     DQLFATLDTTTRRLWIDGAGQVVVSDTVGFIRDLPPTLIAAFRATLEETIHADLLLHV
                     VDAASPQRDEQIAEVDKVLAEIGAGETPCIRVYNKIDMAGYAPRVERDEHGTIVRVFV
                     SALERDGLDGLRGAIVESGRIAGNNASSIEHV"
     gene            13428..14774
                     /gene="hflK"
                     /locus_tag="VFR20_02900"
     CDS             13428..14774
                     /gene="hflK"
                     /locus_tag="VFR20_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEU0229496.1"
                     /translation="MRLLSNMFNLNDPGWGRGDGNNGGSEPPRRPGKGDGPPDLDEVW
                     RDFNNRLGSLFGRKPRRGGGFGGNGGGGTPVMLPKGSPKLAALVILVIIVLWLASGFL
                     IVQEGQVAVVTRFGKYTKTLPPGLQWRLPYPIEDSQMVNISRLRTFEIGFRGGATNKV
                     LPEALMLTNDENIVDVQFVVQYRLKPGGAPDYLFRTSHPDEAVRQAAETAMREVVGKK
                     PMDHILYSGRAEVAAAVQKRIQSLLDEYQTGIEVSTVAIQNVQPPEQVQAAFDDAVKA
                     GQDRERQVNEGTAYAKKVIPEADGEAARMIQQAEGYKAKVIGDAQGNTARFLSIQSEY
                     AKAPEITRERLYLSTMQDIFQHTSKVMIDANASNNLLYLPLDRLMKEAANEAATQSAA
                     ASAVATDASSTSSSGSSASPSKGSRKSASDTARHDSSLPSVPDSLRDTLSLDRFSH"
     gene            14789..15700
                     /gene="hflC"
                     /locus_tag="VFR20_02905"
     CDS             14789..15700
                     /gene="hflC"
                     /locus_tag="VFR20_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEU0229497.1"
                     /translation="MQRFFPALIGLIVLVGILSSCVFTVGERDDALVFSLGEVRKTIT
                     EPGLYFKLPPPFENVVLLDKRLQTIETNQPERIQTSEKKNLLIDSFVKWRISNPRLFY
                     VTFGSNTRAAQERLQAQIRDALNASVNVRTVKDVIANQRDAILKEVLGAVQRRAKPLG
                     VDVVDVRLRRIEFAPEISESVYRRMEAERKQEANRLRATGAADSEKIRAEADRQRQEI
                     LAKAYAKAQTIKGEGDADASSIYAKAYSKDPKFYSFYKSLLAYRDAFSKPTDLLVLSP
                     KSEFFRFWNSSTGGATGGGALKAGAAH"
     gene            15949..17112
                     /locus_tag="VFR20_02910"
     CDS             15949..17112
                     /locus_tag="VFR20_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEU0229498.1"
                     /translation="MSNWLLPENLADVLPAEARRIEELRRELLDLYRTYGYELVAPPL
                     AEYLDSLLSGAGHDLNLRTCKLVDQLSGRTLGVRADMTPQVSRIDAHLLNREGVARLC
                     YCGSVLHARPAGLLSERELLQIGAELFGHAGVEADIEIIQLALESVGRAGIGHARLDL
                     NHPGIVHAIVRADPALEGVEEDVFQLLCAKDRPGLVELGQRLPGLRADSLDALCALTS
                     LYGGTEVLDEAWRVLPPLPTLRAALGDLRTLVAALPHHEFSVDLADLGSGYGYHSGVV
                     FSVYAQGWHDALVKGGRYDGMGKAFGRARPATGFSLDLRKLSAGLPTAPLSRAIRAPW
                     GTAPDLLEAVRALRGAGEVVVQALPGQAYQMDEFSVDRELVVEDGVWRTRAIE"
     gene            17193..18488
                     /locus_tag="VFR20_02915"
     CDS             17193..18488
                     /locus_tag="VFR20_02915"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU0229499.1"
                     /translation="MSKNIVIIGTQWGDEGKGKIVDWLAESAQGVVRFQGGHNAGHTL
                     WINGKKTILRLIPSGIMHPNVTCYIGNGVVLSPEALLTEIAELEQAGLEVRSRLQISP
                     ACPLILPYHVALDQAREARKGPGKIGTTGRGIGPAYEDKVARRALRLQDLYDPAIFDE
                     KLAEVLDYHNFVLTNYLGGKALSATEVRDKVMALAPRIEPMVADVSNNLSIAQKAGHN
                     LLFEGAQGALLDVDHGTYPYVTSSNCVAGAAAAGAGVGPQSLHYVLGIAKAYTTRVGS
                     GPFPTELLDDTGARLATVGKEFGSVTGRPRRCGWFDGAAMKRSVAINGISGLCITKLD
                     VLDGVEEIKIGVGYRYKGQFLDVLPYGAHAAAEAEPVLEEMAGWPESTVGITEYDKLP
                     VNARRYLERISDVCEVPIDMVSTGPDRLETIVLHHPFNG"
     gene            18739..19281
                     /locus_tag="VFR20_02920"
     CDS             18739..19281
                     /locus_tag="VFR20_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEU0229500.1"
                     /translation="MSLPQSTDRDLWVSWDDYHSLIERLASNVHESGWQFDKILCLAR
                     GGMRVGDVMSRIFDVPLGILATSSYREAAGTLQGQLDIAQFITITRGTLDGRVLMVDD
                     MVDTGLTFNRVHQHLLSQFPAISEMRSAVLWWKGHAQVHPDYFVEKLPTNPWIHQPFE
                     DYDSLRPHQLEAWVRKGVRG"
     gene            19422..19634
                     /gene="rpsU"
                     /locus_tag="VFR20_02925"
     CDS             19422..19634
                     /gene="rpsU"
                     /locus_tag="VFR20_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU0229501.1"
                     /translation="MPIVRLKENEPFEAALRRFKRTIEKNGLLTELRSREFYEKPTAE
                     RKRKHAAAVKRHYKRIRSQQLPPRLY"
     gene            19631..21550
                     /gene="dnaG"
                     /locus_tag="VFR20_02930"
     CDS             19631..21550
                     /gene="dnaG"
                     /locus_tag="VFR20_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU0229502.1"
                     /translation="MIPDSFVQDLLARVDVTDVVGRYVPLRKAGVNLLGLCPFHNEKS
                     PSFTVSPTKQFYHCFGCGAHGTAISFLIEHTGASFPEAVRALAATVGMTVPEEARSPR
                     QQAASRQRKEEASRHQKALEQAQTHYLNELKASAAAIGYLKKRGLSGQTAARFGLGWS
                     GTDRRGLAKVFEHYEDPTLVESGLVIESEDGRRYDRFRERIMFPIRDARGRLIGFGGR
                     IIGKGEPKYLNSPETSLFSKGQELYGLWEARAAIRSEGHVLVVEGYMDVVSLAHQGVE
                     NAVATLGTATTAMHVQKLLRASDRIIFSFDGDQAGRRAAWRALQQCLSSLRDDISVRF
                     LFLPPEHDPDSYIQAFGQEAFRATVSEAAPLSRFLLDELASRHQMDEAEGRAACVHEA
                     KPLLAQLPQGALRLQIEREFARLVRLTPEELSSMLDAIPATPGDSPSSGGQAASSRPI
                     RSGQAVSQDEPEGFFADSSPVDDFPPDVFPMGAISTSRTRRPRRAPARMVTPMAKRLL
                     RLLVAHPGLVASLGDQQLEIVCRSPHLVLVRDLIALINGSGSRHVGALLQAAEPGSDL
                     EEVLLSLSAELLAQDDLPDPDAEWNDALARIELDAVKAEQSRLIETGLKDDAARRRYQ
                     ELTRRIALLSGASLR"
     gene            21811..24027
                     /gene="rpoD"
                     /locus_tag="VFR20_02935"
     CDS             21811..24027
                     /gene="rpoD"
                     /locus_tag="VFR20_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU0229503.1"
                     /translation="MTQATGKNSKATKNTDDDKKKVAAASRSVDKTTATAGESKAGKP
                     AATKAARKPEGADAKKAGKGAAKAASRPVPSGRRPGRPAKNANNDAANFSDDDVEPEV
                     IPELKPLPKRGGKRGKGDKDVPARSQMTPEEQEARRNRLKVLIKLGKERGYLTYGEIN
                     DHLPDDLVDAEAIDGIISTFSDMGISVYDQAPDAETLLMSDNAPVASSDDDVEDEAEA
                     ALTTVDSDFGRTTDPVRMYMREMGTVELLTREGEIEIAKRIEDGLKHMVMAIAACPTT
                     VNEILAHVQRVREGTAQIDEIVDGLIDEEGEEYAGAGVSSDSDENEDGPAGGMSSKQL
                     ENLRVNSLRKFELIGQWFEKMRKAFETEGYCSDAYVQSQQEILDELMGIRFTAKMVER
                     LADTLRAQVAEVRKFEREVLHTCVDRAGMPRAYFIKVFPGNETNLNWVLEEVAASHPY
                     SETLERHVPAVQEIQQKLIDLQASVVLPLKDLKDVNKRMATGEAKARKAKREMTEANL
                     RLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRS
                     IADQARTIRIPVHMIETINKMNRINRQILQETGAEPDPATLAQKMDMPEDKVRKILKI
                     AKEPISMETPIGDDDDSHLGDFIEDSGTLSPSEYALHGSMRDVVKEVLDSLTPREAKV
                     LRMRFGVEMSTDQTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRADKLKSFLEGQ"
     gene            24264..24977
                     /gene="pxpB"
                     /locus_tag="VFR20_02940"
     CDS             24264..24977
                     /gene="pxpB"
                     /locus_tag="VFR20_02940"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEU0229504.1"
                     /translation="MISPLLSDPVVEPEGDRCLMLRYGTQLDARVSARCLATAQLIEH
                     ANLPGVFDVVPSFTTVAVHYQPSAFPRDPYETLAAAVLALLEPAAAAPGFEPRTIRVP
                     VCYDDVFAMDLEYVSDTCHLTKQEVISLHTSDRVSVFALGFAPGMPYIGVHHGKLDLP
                     RRATPRTDVPGGSVAIANRQSVIYPYTSPGGWHVIGAMPMPLFDPGKPPYSFLLPGDI
                     VRFTPVSRSEFETLRQDHS"
     gene            24974..25978
                     /locus_tag="VFR20_02945"
     CDS             24974..25978
                     /locus_tag="VFR20_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEU0229505.1"
                     /translation="MSIEVIKAGALSLFQDNGRLGFQKLGVPVCGPMDESAHRLANLL
                     VGNGGDAAVLEITLMGPKLRFTAPAGVALTGADLGARVDGLPLAQNRPLIVRAGQVLD
                     FTGGKSGMRGYLAVHGGFNLEPQLRSCSTYLKGHLGGVDGRALQPGDVIGLRSALSVD
                     PDFLQEAERRLWEVQLYLSARLGLVRRDTLRVVKGPHWGLFSEKSVHDFVTAGFRISP
                     QSDRMGYRIAGPGLAVKKPAQILSEATAFGAVQVPHGGDPIVIMADRQTMGGYPKIAY
                     VASVDLPSLAQSRPGEVVRFNLVSLEQAQALAKARDAAFAELGARLACVSGILSGGSP
                     "
     gene            25975..26742
                     /locus_tag="VFR20_02950"
     CDS             25975..26742
                     /locus_tag="VFR20_02950"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEU0229506.1"
                     /translation="MTLSIDLNCDMGESFGAWKMGHDEAVLPFVTSANIACGFHAGDP
                     ATMRKTVCAALEHKVALGAHPGLPDLVGFGRRNMDLSPQNAYDIVVVQVGALAGVAAS
                     QGARLNHVKAHGALYNMAARNKDLARALAQAVHDVDRDLIFYALASSAMVEAAHEVGL
                     RVAQEVFADRTYQGDGSLTPRSRPEAMIVDSQQSIAQVLRMIREGKVRSVQGTDIAVR
                     ADTLCIHGDQPGAVMFAKSIRAALQAEGIEVRTVGQP"
     gene            26739..27428
                     /locus_tag="VFR20_02955"
     CDS             26739..27428
                     /locus_tag="VFR20_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU0229507.1"
                     /translation="MTAFDSGFTGPDCSIRTYDGEAHTHAHGYAQIMVPLAGRMELEI
                     AGRALFTDMSCGMVVPAGASHAYVARPGTRMLVIDTPEQKGLERVRRFAITAVCASLA
                     DIGDVQRQLSLVLELPTVLSRRGLDLARLDAALDAGLHEDWPVSRMAGLFFLSAQRFH
                     ARLLELTGRTPAAYLRDRRLDAAEKALARGANLEAVAQRVGYRTASALAFALSRDRHV
                     GARSLRRRGVV"
     gene            27584..28477
                     /locus_tag="VFR20_02960"
     CDS             27584..28477
                     /locus_tag="VFR20_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU0229508.1"
                     /translation="MKSSSTTGIILVLCAAVLWGTTGTTQSFASSALSPYWVGALRLG
                     IASLFFCVYAGGRLGGRRLARELLGIPWLWAVLAGACMAGYNLAFFAGVRATGVAVGT
                     ALALGSGPVWAGFLQMVVARRAPGSGWWLGTMLAVAGGILMVLGRGGDLRADATGITL
                     CLVSGLSYAIYALVSKHLVGRNDPGAVTLVVFGVGALIAMPVAWAWVGGPDIAGRGWA
                     VVVFLGVAATGIAYLLFNTGLRGIAAASAVSLALMEPLTAFLLAIVVVGERPAASAFF
                     GLVCVLAGLALVIRAEMRRQP"
     gene            complement(28468..30087)
                     /locus_tag="VFR20_02965"
     CDS             complement(28468..30087)
                     /locus_tag="VFR20_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEU0229509.1"
                     /translation="MLVAQNDYPATYKAFKWEVPEHYNIGVDACDKWADGSGRLALIY
                     EKPTGEQSRYTFDDMKRLSDRFARALLAHGVKKGDRVGIFLPQAVETAITHLAIYKIG
                     AIAVPLFTLFGTEALQFRLGNSGAAALITHAEGRRKLAEVRSSLPDLVTTFDIDADCE
                     EAGWLPFWPELEAQSGEPFTADSLAEDPAVIIYTSGTTGKPKGALHAHRVLLGHLPGV
                     EMSHNFFPKNAALMWTPADWAWIGGLLDVLLPSWHHGVPVLARRFAKFDAETAYDLMS
                     RHGVTHTFLPPTALKMLRTVANPGARWPLKLVSVASGGESLGLELIEWGRRDLGLTIN
                     EFYGQTECNMIVSSCSALFDPLIGTMGRAVPGHDLAIVDDHGHPVADGTEGDIGVRAP
                     DPVMFLNYWKNPEATREKFAGPYLITGDRGIRDANGFIQFVGRNDDVITSAGYRIGPG
                     PIEDCLLSHPAVKMAAVVGVPDTQRTEVVKAFIILNDGYSPSDDLVAEIQDHVRRKAA
                     AHEYPRQVSFVQSLPMTATGKIIRKQLRAQG"
     gene            complement(30345..31202)
                     /locus_tag="VFR20_02970"
     CDS             complement(30345..31202)
                     /locus_tag="VFR20_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU0229510.1"
                     /translation="MKLLRHGPVGQEKPACLDAQGKLRDLSGVIPDISASTLAPEQLD
                     ALRAVDVNSLPVITNPGRLGVPYAGVGKFICVGLNYSDHAAETGAAIPSEPILFNKWS
                     AATGPNDPIVMPKGSVKTDWEVELGVVIGRKARYVGVDEALDYVAGYCVVNDVSEREF
                     QLELGGTWDKGKGCDTFGPVGPWLVTADEIPDPQKLGMWLDVSGKRFQNGNTSTMIFG
                     VAHLVHYISQFTTLNPGDLISTGTPPGVGLGQKPPLYLKAGDVVHLGIDGLGEQRQTV
                     HAWDASLLD"
     gene            complement(31225..31971)
                     /locus_tag="VFR20_02975"
     CDS             complement(31225..31971)
                     /locus_tag="VFR20_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0229511.1"
                     /translation="MTNRLTGKTAVVTAAGQGIGRATAEAFAAEGARVIATDINPHTL
                     AHIQDCETRVLDVTRPDQIATLAHELGSVDVLFNCAGYVHDGNILGCEPDAWSFSLEL
                     NVTSMYHMIRAFLPGMLQQKRGSIINMSSAASSIKGVPNRFVYGTTKAAVIGMTKAIA
                     ADYVSQGVRCNAICPGTIESPSLRERIASQAAKYGKSEKQIHDDFVARQPIGRVGRPQ
                     EIAALAVYLASDESSFTTGTSQVIDGGWSN"
     gene            32396..33328
                     /locus_tag="VFR20_02980"
     CDS             32396..33328
                     /locus_tag="VFR20_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU0229512.1"
                     /translation="MKRVSATTKLVRAGDGSSVQSDSASRGMADAQNGDSDAKNGGPG
                     ASAGTTRYAAPALEKGLDILEALSVSGEGYTLNELCQTLGRNASELFRMVVTLQRRGY
                     IEAGPGDRYTLTLKMFQLAHRQPSLRSLTDVALPLLRELSERARQSCHLSIFQDGRVV
                     IIAQVDSPERWSFGLKVGVAMGLVDTSSGHVLLASQNEVERARMLGGHVRVEGELAMD
                     PGQLFPLLDAARTNGYASMPSVQIQGVTNIAFPIRGPGGRVVAAVNVPHIARIDGQHR
                     PDVDQIKVIMADVCGRLSARLGYDAGQAAPRGDL"
     gene            33339..34091
                     /locus_tag="VFR20_02985"
     CDS             33339..34091
                     /locus_tag="VFR20_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008347988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU0229513.1"
                     /translation="MDAFLLVFGAGLLAGAMNALAGGGSFVSLPALIAVGIPSVQASA
                     SGAVALFSGGAASAWAYRDGLGPIGPVSVRAIFLATVVGGAGGAILLLLTPTKVFDVL
                     VPWLMLLALLALAFGKRLGEWLRQRYRIGAAAVLAIQFVLGAYGGYFGGAVGLMMMAV
                     WGLLESRDLKSLSAPRTLMVVAARTIAILIFIGADAVRWPETLVMLVAAVIGGYVGAL
                     AGRRAPAGLIRGGTLLLTTGITVAFFLRAYGG"
     gene            34160..34806
                     /locus_tag="VFR20_02990"
                     /pseudo
     CDS             34160..34806
                     /locus_tag="VFR20_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012327929.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34901..35932
                     /locus_tag="VFR20_02995"
     CDS             34901..35932
                     /locus_tag="VFR20_02995"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HEU0229514.1"
                     /translation="MQKLDRRTLLRTGVVVGSTALLGARGLRAAWADEARTLTLYNGQ
                     HTHTTAALVDAFTKSTGIKVAIRKGSSSQLANQIIEEGSHSPADVFYSEESPPVVKLA
                     EKGLLAQLDPKTLKQVRPGYTSRKGDWIGVTARCRVIAYNTNMVKESELPRSVLDMAT
                     AKWKGKVAFVPTSGAFQEQIVAIEQLKGRAVALKWLEGLKKYGVVYNGNMAAMKAVER
                     GEIATALINNYYWFVVAQEVGAKNMHSALYYTGDKDPGALITVSAAGILKTSKKQALA
                     QKLMAYMVSPEGQTVIAASVAEYPLRPGIKSPYDVKPFDELDPPPITPADLGDAGDAL
                     ILRREAGLA"
     gene            35955..37502
                     /locus_tag="VFR20_03000"
     CDS             35955..37502
                     /locus_tag="VFR20_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU0229515.1"
                     /translation="MNFSGSQRRGGPSIWFAGSALVPAALISLPLVYVALRATQAGVV
                     GIHTELFRSRTLMLLINTLVITVSVTILSSLIGLAVAWCVERTDLPGRRVWRILAGVP
                     LAVPAFVSSYAWSSISPMFQGMAGAILVLSLASYPLVYLPVAAGLRGVDPGFEDVARS
                     LGDGPWRCFLRAVLPQLRPALGGGALLVLTHMLAEFGAFSMLRVQTFTTAIFESYQLQ
                     FDSSSAALQSIVLMALCLPAAYGEMRLRAGTRVARVGRGSARAASTVRLGRLRHGALA
                     GFVALVVLALGVPLATLAYWLAVGHSAGRGYAEILPAIGGTVALASTGAVVTSLFAVP
                     LVLLSLRHRGWLAQLADRLPYIIHGLPGVVVALAMVFMSIHLVPALYQGVAVLLLAYA
                     ILYLPLAQSSLRASAELVSPQLEIVARGLGRRPLAAFLTATLPNIAPGIGAALALMVL
                     EIARELTATLMLAPTGVTTLATQVWSHTYDAEYAAAAPFAALLVLISIVPVYFFTQRF
                     LQFGESK"
     gene            37499..38536
                     /locus_tag="VFR20_03005"
     CDS             37499..38536
                     /locus_tag="VFR20_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0229516.1"
                     /translation="MSRIHVDQVDKSYEGNTVLHGVSLDIDDGSVVALLGPSGCGKTT
                     LLRLLAGFDWVDSGTIRLDDRVVAAPTLHVPPERRGVGYVPQEGALFPHLDVQGNVRY
                     GLSGRADWRARVGEVLALTGLSGLERRYPHELSGGQQQRVALARALAPRPALILLDEP
                     FNALDLDLRRGMCEDVITLLRKTGTTTVLVTHDPAEAFAVSDRIAVMHEGRIMQCAEP
                     DVVYWRPASAPVARLTGSVVFLDGTVNDGHVETPVGPVPLYPSRAMPAGSACAMLRPE
                     QITLGPPGRGIRARVAKRTFHGDHVLLGVVVDGLLELNLRMTSWAAPASGSEIFLSVH
                     GSCVAFAPDDA"
     gene            complement(38538..39299)
                     /locus_tag="VFR20_03010"
     CDS             complement(38538..39299)
                     /locus_tag="VFR20_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0229517.1"
                     /translation="MNLGAVKAIYQFEMARTWRTLMQSIVAPVISTSLYFVVFGAAIG
                     GRINQIGGVSYGAFIVPGLIMLSLLTQSISNASFGIYFPKFTGTIYEILSAPVSYFEA
                     LLGYVGAAATKSVILGVIILATACLFVPVRIDHPAVMILFLLLTAITFSLFGFIIGIW
                     ADNFEKLQLVPLLIVTPLTFLGGSFYSIDMLPPFWQKVTLFNPVVYLISGFRWSFYGL
                     ADVSVPVSLAMTLVFLAICLGIVAWIFRTGYRLRA"
     gene            complement(39296..40219)
                     /locus_tag="VFR20_03015"
     CDS             complement(39296..40219)
                     /locus_tag="VFR20_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0229518.1"
                     /translation="MPAIITISNLDKTYASGHRALKNINLDIRQGEIFALLGPNGAGK
                     TTLISIICGLVNPTRGTVLADGHDIITDYRAARARIGLVPQELTTDAFETVWNTVSFS
                     RGLFGKPTNPAHIEKVLRDLSLWEKKDNKLLTLSGGMKRRVLIAKALSHEPSILFLDE
                     PTAGVDVSLRKGMWDMVRSLRATGVTVILTTHYIEEAEEMADRIGVIDHGEIVLVEEK
                     ATLMRKLGKKQLILRLQKPLSQIPDALTSWPLAIADGGDLVYTYDAQQEHTGIPALLA
                     AVGAQGLAFKDLETSQSSLEDIFVDLVKEHA"
     gene            40421..42796
                     /locus_tag="VFR20_03020"
     CDS             40421..42796
                     /locus_tag="VFR20_03020"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEU0229519.1"
                     /translation="MSFCTIPDKFRCRMGALGMARWFAACLLAVLLGIAIQPARAAVP
                     SAQSARSSAAPSYGALASLLENKDSRDALIKQLRTLAAKSEGKLSGQQAPPVPTGETA
                     GGQAGFLAGAGDMAATLQSFTVSFSHNVETMADMFSRRGWVAGAYVKGLETVPGLLAV
                     IASTIIALFVLRACAMWAYRRLDDWVSRLGADLATTGHRALAPRELYCKMGAIALGFL
                     VDLATVLLAAAVGYGVVLYGVVRHLSAQAPGAVFVSAFLAVEATCALARVVFATRYPD
                     LRLIRMTDGVAGFWNAWLARLIRITGYGLLVAVPVAQTLLLSAAGKVLGLLIMLGVYV
                     YAVRVIWTNRHLLRERMMARAAELSAPLMSTLLRVLARIWHVLGIAYFTELLIASQVN
                     PEDALPFMAQATIQTLAAVGVGVLLSLILTAMLSRRIHLSEDMRARLPMLENRINAYV
                     PATLRALRLFILFVVALVVLDAWHAFNLAAWMVSASGRAVLTTVVRVAVILLAAAFVW
                     TVVASIIESRLSGGTGRHRPSDREKTLLSLFRNASLIVIITFTVLVVLSQVGIAIGPL
                     IAGAGVVGLAIGFGAQKLVQDVITGVFIQLENGMNQNDVVELAGLFGTVEKITIRSVV
                     VRTLDGGYHLIPFSTVDKLTNHTRDFGYHYGEYNIGYREDADNVIAHLEHAFDELMQD
                     SELAPHVMERISIPGVTSLTERGFTVRVLIKTVPGMQWMIQRGFNRLVKKHFDAAGIE
                     MPYPHSVVQFEGGRDGGMVPLRVRMGQSRSEGPRAEGSGSCAPHPAPQGTA"
     gene            42894..44375
                     /locus_tag="VFR20_03025"
     CDS             42894..44375
                     /locus_tag="VFR20_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU0229520.1"
                     /translation="MPAIGGALLTSEQMRRVDQAAVASGVPGIELMEAAGAAVARRVA
                     GRWNRRPVLVLCGPGNNGGDGFVAARHLVDAGWPVRVGLLGSLARLAGDAAHHAEQWG
                     GTTVDADPSLFEGAGLVIDALFGSGLSRPLDGLAADLVRALGASAIPVCAVDVPSGLS
                     ADTGEVLGVAVAADETVTFCRKKPGHLLAPGRQLCGVLTVADIGIPEAALDTVSPRVF
                     QNGPGQWLDQYPWCRPDTHKYRRGHVLVLGGAVQTGAARLVALGAARAGAGLVTVAAP
                     DSVWPIYAAALTSVMAQPVHGPDGFRALLADKRKNVVVLGPGAGASPETRQCVLSALE
                     TGRGVVLDADALTVFSGEVEVLADAIAGPCVLTPHEGEFERLFSLRGDKLARARFAAR
                     QSGAVVVLKGWDTVVAAPDGRAVVNANAPPELATGGSGDVLAGLIGGLMAQGMAPFQA
                     ASAAVWLHGEAATQIGPGLIAEDLPAALPPVLGRLKALNIGLH"
     gene            44431..45279
                     /locus_tag="VFR20_03030"
     CDS             44431..45279
                     /locus_tag="VFR20_03030"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU0229521.1"
                     /translation="MSDVIALVFDFDDTLAPDSTSGYLARMGVDVTRFWTEEVGPMLS
                     QDDWDPVPAYLYQMIRLSNSGNYGRFTRESLMAWGRDLPLHDGVDTLFERLRATVRAQ
                     HPQIQLEFYLISSGIGDVVRSTPIAREFTDIWASEFKYDPDGGIEFPRRVVSFTDKTR
                     YLFHIQKGIIGPTSRNKPFEVNRKVPEDKLRVPFEQMIFVGDGYTDIPCFSLIRRSGG
                     VAFGVWDPRHKEKRSRAWGFIQEGRVSNLNQARYGDDAELYQWLEEAVDSLASRIAIN
                     SRMYRG"
     gene            45305..45802
                     /gene="tadA"
                     /locus_tag="VFR20_03035"
     CDS             45305..45802
                     /gene="tadA"
                     /locus_tag="VFR20_03035"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEU0229522.1"
                     /translation="MAASPAFSAQDTEHMLRALEQARLASLQGEVPVGAVVADENGAC
                     IAEGFNSTVGAHDPTGHAEIVALRRAAQRMGNYRLPGKTLYVTLEPCVMCMGAMLHAR
                     LARVVYGAADPKTGACGGVLSVHAVVELNHQTRVEGGLLAEPCGDILRRFFRARRGGG
                     IGAQP"
     gene            45910..46827
                     /locus_tag="VFR20_03040"
     CDS             45910..46827
                     /locus_tag="VFR20_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU0229523.1"
                     /translation="MTHSHDHSEPPRGIYLISPSGQVRDPADLARACERLSTLGFKTK
                     VDRAALAVHERFAGTDKQRLAGITRALRQPHPIVMATRGGYGLSRLLPLIDWPAVAES
                     GKTFIGQSDFTAFNLALLARTGVESLAGPTAIADFGGATVDDLTEALFTELLDHQLEI
                     LSFETPDADPVDARGVLWGGNLAMVAALVGTPYMPKVRGGILFLEDVAEHPFRVERAL
                     AHLWHAGILDRQKAIVLGHFTDYRLGPHENGFDMPSVLRWLRATVKVPVVTGLPYGHI
                     PTKATLPVGRKVGIATEKGTAHLVLHEHA"
     gene            46976..48571
                     /locus_tag="VFR20_03045"
     CDS             46976..48571
                     /locus_tag="VFR20_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEU0229524.1"
                     /translation="MISTSNLTIQFGPKPLFENVSVKFGGGNRYGLIGANGSGKSTLM
                     KIIGGDLEPSSGNVALDPGVRLGKLRQDQFAFEDLRVLDVVMMGHHEMWQAMIDRDAI
                     YANPDATDEDYMRAADLEARFAEYGGYTAEARAGELLLGLELTIEQHNQPMREIAPGW
                     KLRILLAQALFSNPDVLLLDEPTNNLDINTIRWLENVLNGYQSTMIIISHDRHFLNEV
                     CTHMADLDYGEVRVYPGNYDDYMLASTQARERVTAANAKAKERVADLQDFVRRFAANK
                     SKSRQATSRLKQIDRIKADTVEVKPSSRQNPYIRFEQEKALHRLAITTESISKAFDKP
                     VIRPFSTMIEAGQKIAIIGANGVGKTTLLRLLAADLEPDSGSLKYAENADLGYMAQDV
                     SGQFQSDSNLFDWMGDYRKPGDDDQAMRSVLGRLLFSSDDIPKKVRVLSGGEKNRMSF
                     GRLMLGRHNVMLLDEPTNHLDMESIESLQYALEKYAGTLVFVSHDRQFVSGIADRVIE
                     IMPDGDIVDYRGNYDDYLASRGL"
     gene            complement(48721..>49018)
                     /locus_tag="VFR20_03050"
     CDS             complement(48721..>49018)
                     /locus_tag="VFR20_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU0229525.1"
                     /translation="GPCRSFAPTFEAASGQHDDIVFAKVNTEEQQEIAAGMGIRSIPT
                     LMVFREKVLLFSQAGALSPTQLNELITQVKAVDMAKVHEEIAQQEQANGANAST"
BASE COUNT         8528 a        14881 c        16096 g         9513 t
ORIGIN      
        1 gatcgcttcg acctgggcct tgagttccgc cacgcgcgcc tcggcttcgg cgtcgatcag
       61 ggcgggattg accacagggt agggctggac actgatactg gtttcttcat cggcgccacg
      121 cttgccggcg tcgacggcca ccttctgcca gagctcttcg gtgatgaacg gaatgatggg
      181 gtgggcgagg cgcagcaatg tttccagcac ccggatcaat gtgcggcgcg taccttgttg
      241 ttcctggggc gttccgtgct ggatctggac cttcgccagt tccacgtacc agtcgcagta
      301 ttcatcccag atgaagtggt acagggcgtt ggcgatattg tcgaaccggt attcggtgaa
      361 cccgcgctgg acctcttctt ccaggcgctg cagggcgctg atgatccagt ggtccacgaa
      421 cgacagcgtg ccccgatcgc caccagtgag atcatggccc gacgtgttca tcagcacgaa
      481 tcgcgtcgcg ttccaaagct tgttgcagaa gttgcgatag ccttcgcagc gcttgagatc
      541 gaaattgatg ttccggccca gtgtggcgta ggcggccatg gtgaagcgca gcgcgtcggt
      601 gccgtacgcg gggaacccct gggggtagtc cttgccggtt tttttccgga tggcggcggc
      661 ctgcttgggg ttcatgagcc cggtggtgcg cttgtccagc agggcttcca ggctgatgcc
      721 atcgatcagg tcgaccgggt cgatggtgtt gcccttggac ttgctcattt tcttgccgtc
      781 catgtcgcag accaggccat gcacgtacac cgcccggaac ggcacgtggc cggtcaggtg
      841 ggtgctcatc atgaccatgc gcgccaccca gaagaagatg atgtcgaaac cggtgaccag
      901 gacgctggaa ggcaggtagc gttccaggtc gggcgtgtcg cgcggccagc ccaggtcggt
      961 gaaaggaacc agggccgacg agaaccaggt gtccaggacg tcttcgtcgc gtgtcagcgg
     1021 ttcggtcaca cccgcggccg cggccttggc gcgcgcttcg tcggcgtccc gcgcgacgaa
     1081 gatgcgaccg tcgggggcgt accaggcggg gatctgatgg ccccaccaca actggcgcga
     1141 aatgcaccaa tcctggatgt tgttgagcca ttggttgtag gtggtggtcc agttctcagg
     1201 gtagaacctg atgcggccgt cggccacgac gtccagggcg acctgggtga tgctcttgcc
     1261 ggggtgcagt gtgccttcgg gagccggttt gctcatggcc acgaaccatt ggtcggtgag
     1321 catgggttcg atgacggtgc cggtgcggtc gccgcggggc accatcagtt tgtgcggccg
     1381 aacctgccgc agcaacccca atgccgccaa gtcgtcgacg acccgcttgc gcgcctcgaa
     1441 gcggtcgagg ccgtgatagg cggccggcgc cgcatcgctg atatgggcgt cgagggtcag
     1501 gatgctgatc atttccagcc cgtggcgctg tccgaccgca tagtcgttga aatcatgggc
     1561 gggcgtgacc ttgaccacgc cggtgccgaa ttcacgatcg acgtaggcat cggcgatgac
     1621 ggggatgtca cgatcggtca gtggcagcct gacgcggcgt ccgatgaggt gggcgtagcg
     1681 ttcgtcttcg ggatgcacca tgacggccac gtcgcccagc atggtttccg gacgggtcgt
     1741 cgcgacgacc agatgggtga gcgcgcccac gggctcgacc aatggatacc ggatttccca
     1801 catatggccg tcttcttcct cgctgacgac ctccaggtcg gagaccgccg tgcccagaac
     1861 ggggtcccag ttcaccaggc gcttgccccg gtaaatgagg ccttgctcgt acaggcgcac
     1921 gaaggtttcg gccacgcctg ccgacaggtt ggcgtccatg gtgaaatact cgcgcgacca
     1981 gtcggccgag gatcccagac ggcggaactg ctcggtgatg gcgctgcccg acttctgttt
     2041 ccactcccag actttctcgg tgaacttttc gcggccgagg tcgtggcgtg acacttgctg
     2101 ggcgtccagc tggcgctcca cgacgatctg ggtggcgatg ccggcgtgat cggtgccggg
     2161 aatgaacacg gtgtcatcgc cccgcatgcg atggtagcgg accaggccgt ccatgaccgt
     2221 ctggttgaag gcgtggccca tatgcagcgt gccggtgacg ttcggagggg gaaactgcac
     2281 gacaaagggg tggctttccg tgcccgccgc cggcgccacg tgctggccgc ccttgaaata
     2341 tccacgttgc tcccattgcg cgtaccagcg cgattcgatt tcctggggtt cgaaactttt
     2401 gggtggttga gaggcgtcgt tctgggagga ggtcggggtc atggagcgta tgtcgggttg
     2461 cggcgggtgg cgtgccccgg aaaggaggaa aacaagggcg cgccatgagg gcgcgccggc
     2521 ctccgatggg acggcgtcgg gggaactatt ttaaagggta gcggcccgtc cttgacatgc
     2581 taaaatggca gattcacgaa aactttgtcg aagttgttgt ttttaatggg tttaccttgg
     2641 ttgttcacct gcgttgctta cctgggttgc caggccgagc ccgggtaccg gaacccgtca
     2701 tttcactgga taatacatgg ctattgaacg cactctttcc atcatcaaac ccgacgccgt
     2761 ggcgaagaac gtcatcgggc agatcgtcgc gcgcttcgaa caagcggggc tgaaggtcat
     2821 cgccgcccgg ctgcaacaat tgtcgcgctc ggatgcggag cgcttctacg ccgtgcacaa
     2881 agagcgtccc ttctaccgcg atctggtcga cttcatggtg tcgggtccgg tgttcgtgca
     2941 agtgctcgaa ggtgacaacg ctgtccagaa gaatcgtgac ctgatgggtg ccaccgaccc
     3001 caagaaggcg gcccccggca ccatccgcgc cgatttcgcc gacagcatcg acgccaatgc
     3061 cgtgcatggc tccgacgcgg ctgaaaccgc cgcggtcgaa gtcgccttct tcttccccga
     3121 atacgccatc ctcagtcgct gatacccggg cgcgccctgc actggcgcac cgcatcagtc
     3181 tcccgaaccc gatatgccaa ccgtcgtcga accgatcaac ctgctgggac tggattccgc
     3241 cgcgctcacc gacctggttg gtcagtgggg cagcaagcca ttccgcgcgc ggcagctcga
     3301 acgctggatt caccagcgca gtgctgattc gttcgaccag atgaccgacc tggcgcgcga
     3361 cctgcgccaa cagttgtcgc agcgctgcct gatccgtgcg ccccgggtgc tcaccgagca
     3421 cctgtcggcc gacggtacgc gcaaatggct gttcgacgtc gggcgcaaca acgcagtcga
     3481 ggccgtcttc attcctgaag acgatcgcgg cacgctttgc atttccagcc aggcgggatg
     3541 cacggttgcc tgtccgttct gctcgacggg ataccagggg ttcaaccgca atctcaccac
     3601 agccgagatc atcggccaac tgtggcatgc caagcgcgcc gtcgaggccg accccgcgcg
     3661 tgcgcgaacc agtcatgccg acgatgcgcc gatgcgggcg atcagcaacg tggtcatgat
     3721 gggcatgggc gaaccgctgc tcaattacgg ccaggttctc ggggcgctgc gcctcatgct
     3781 ggacgacaac gcctatgggc tgtcgcggcg ccgggtcaca gtgtccacgt cgggcgtggt
     3841 gcccatgatg gatcgtctgg cggccgattg cccggtggcg ctggccgtgt cattgcacgc
     3901 gcccaacgac gccttgcgcg atcgcctggt gccgcttaat cgcaagtatc cgctcgccga
     3961 tctgttggcg gcgtgcaacc gctacctgga acacgcgccg cgtgatttca tcactttcga
     4021 atacatcatg ctcgacggtg tcaacgacac cgacgatctg gcgcgcgaac ttgtcggcat
     4081 ttcccggcag gtgcgttgca agttcaatct gatcccgttc aatccgttcc cgcaatccgg
     4141 cctgaagcgc tcgccgccgg cacgcgtgcg gttgttcgcg cagcgcctga tggacgccgg
     4201 cgtggtcacc acggttcgca agacgcgtgg cgatgacatc gctgccgcct gcgggcagtt
     4261 ggcgggtgac gtcaaggata gaacgcgtat tgatgagcgc ctgcccgatc gggcggtgat
     4321 cgctcgcaac caggagtgcg gatgacccct ccctcagcca acagagccag cctggacgac
     4381 gatcctgttc tgtccagacc cgacgccggc acggatattt atcaaggccc ccaggggctg
     4441 gggcccacgc tccgggcgtt gcgtgaggcc aagcgtattt cgctcgccga ggtttccggc
     4501 cggctgaagt tttccgtgcg gcagttcgag gcgctggagt ccgaaaaatg ggatctcctg
     4561 cccacgggtg tgtccttgcg gggctttgtg aagaattatg gacgtttcct ggaagccgac
     4621 atcgatgcgc tcatcaccat gctcgagaac caggtcggcg gctcccgcac gcgtccgacg
     4681 gcggtggtcg atgcgccctc gttggcgagt ggcggcgacg tccccatgca tcccgatgcc
     4741 acaacgcatt cgtggggctg gctggtcatc attgtcctga tcctcctcgt cgccgggttc
     4801 tacgccatcg agcgcggctg gatcccgaat tcctggctgg tgttcgactg gctcaagtct
     4861 ttgaagaaat gaattccatc cccgattcca tcactgatgc cgatgtgcca tcaccggtgg
     4921 gggcggccgc acgacgcaag acccgccccg ttgtcgttcg ctggggtcgg catgccgtgc
     4981 ggatcggtgg cgacgcaccc gtcctggtgc agtccatgac caataccgat acggctgatg
     5041 ccaccgccac ggcgaaccag gtgcgtgatc tggcgcgcgc cggctccgaa ctcgtgcgca
     5101 tcacggtcaa tacccccgag gcggcgcgcc aggttgcggc catccgggaa aagctcgatc
     5161 gcatgggcgt ggcggtgccg ctggtgggcg acttccatta caacggccat cgcctgttgt
     5221 ccgattttcc cgattgtgcc caggcgttgt ccaagtatcg aatcaacccc ggcaacatgg
     5281 ggggtggcaa gaagcgcggc gacaatttcg cccaaatgat cgaggtggcc tgtcgccacg
     5341 acaaaccggt gcgcattggc gtgaactggg gcagtctcga tcaagagctg ctggctcgca
     5401 tgatggacga caacaaccgc cgcgcacagc cttgggatgc ccgggcggtc atgcgccgcg
     5461 cgctggtcgt gtcggcggta tccaatgcgc gccgcgcgga agagctcggg ctgtcgccca
     5521 atggcatcat cctgtcgtgc aaggtcagcc acgtccagga cctcatcacg gtctaccagg
     5581 atctttccgc acagtgcgac tatcccttgc atctcgggct caccgaagcc ggaatgggca
     5641 gcaaaggcat cgtggcgtcc actgcggggc ttgccgtgct gttgcagcaa ggcatcggcg
     5701 acacgatccg catatccctc acgcccgaac ccggcggtga ccgaactcgc gaagtggtcg
     5761 tcgcgcagga aatcctccag agcctgaacc tgcgcgcgtt cacgcccatg gtggtggcgt
     5821 gccccggttg cggccgcacg agcagcacgg tgttccagga gctcgccgca agcatccagg
     5881 gatacctgcg tgaacagatg ccggtctgga aaacgcaata tcctggtgtc gagaccatga
     5941 acgtggccgt gatgggttgc gtggtgaacg gcccgggcga gagtcgtcac gcggacatcg
     6001 gcatcagcct gccgggaacg ggcgaggtgc cctccgcgcc ggtctatgtc gatggccagc
     6061 gggtggtcac actcaaaggc gactcgatcg ccgaggagtt cgaggctatc gtccaacgct
     6121 atgtagagcg tcgatacggt ggtggcgatc gcgacggcgg tcccgccgac gagacaacac
     6181 actagtgatc cacgacacta gaccctcagc cgaatttccg cgccgggtcg cgcaggccgc
     6241 gccggtatca accagagtac tcgtagatct atgacgcagt cgtttcagaa agtgcgtgcc
     6301 ctgaccggca tgaaggatgt cctgccggac accagcgcgc agtgggaatc gcttgaatcc
     6361 acggtgcatg agtggcttgg cgcctatggc tatcgcaaca tgcgcacccc cgtgcttgaa
     6421 cagacccggt tgttcgcgcg cggtatcggc gaagtcacgg acatcgtcga aaaagaaatg
     6481 tattcgttca cggattcgct caacggcgaa caactgacga tgcgtcccga attcaccgcc
     6541 ggcatggtgc gggccaccat cgagcacaac atcctgtacg atcggccgca gcgtgtgtat
     6601 gcgatggggc cggtgttccg ccacgaacgg cctcaacgcg gacgctatcg ccagttccac
     6661 cagatcgacg tcgaggccct gggtttcgcc gggcctgaca tcgacgccga actcgtcgtg
     6721 atgctggcgc gcctttggcg gaaattgggc ctgaccgacg tccgcctgga actgaattcg
     6781 ctgggacagg ctgatgagcg ggccgcgcac cgcgccgccc tggttgccta tctagagcgg
     6841 catcagtccg ttctcgacga cgaggcgcgc cgccgcatgt acaccaatcc gctgcgtatc
     6901 ctcgacacca agaaccccgc catgcagggc ctggccgata acgcgccgcg cttgagcgat
     6961 ttcctgggcg ccgcgtcgcg cgggcatttc gatggggttc gcgagcgtct cgacgaggcg
     7021 ggtatcgcat accggctcaa cccgcgtctg gtgcgaggcc tggactatta caatctgacg
     7081 gttttcgaat gggtgaccga catgctcgga acccagggca cggtgtgtgg tggcggccga
     7141 tacgatggcc tgatcgaact gctgggcggc aagccggcgc cggccgtcgg tttcgccatc
     7201 ggcatggaac gcctgctcga attgtgcgaa caagtggcgg ccccgcttga tcggtcagaa
     7261 tgccaggtgt acgtggtgca tcaatccgat gcggcgcaac gaacggcggc gctggcggcc
     7321 gagcggttgc gcgatgccgg gttgcgtgtc atcgtgcatg ccggctcgtc gggcttcaaa
     7381 tcgcagttca agcgtgccga tgccagcggc gccgccgcag cggtgatttt cggtgacgaa
     7441 gagttgtcat cggggaccgc cagcataaaa tggctgcggt ccgacacaac accatcgcag
     7501 gcccagcagg aatcggttcc tattgagcgc ttggttgacg aattgaaaca aagggtttga
     7561 ggcatggctt acgacttgga agagcaggag aagatcgacg cactgcgcgc ttggtgggat
     7621 aaatacggca cgctgtgttc ggtggtactg ttcgccgccg tgctggcggt gctgggctgg
     7681 cgaggctggc agtggtacca ggggcatgaa gccaatcagg cgatgggcta ctttgaagcg
     7741 cttgaaaccg ccgccggcca gccgggtgac gatgccgtgg cgcggatcaa ggcggccagc
     7801 aagaccttgc gcagcgacta tgcctccacc ggctacacgc cgcgcggggt gctggtggcc
     7861 gccgaggcct tgcagcgtcg gaacgatctc gccggtgcgc gcgaacagct tgagtggctg
     7921 atccaccatg gcaaggattc cgccctggcc gcgctcgcca ggctgcggct ggccggcatc
     7981 ctgctgcagc agaaacagta cgatcaggct ttgacccagg tgcaaaacgc gccaacggcc
     8041 ttcgccgggt tgtacgccga tcgccgcggt gacattctgg cggccgaagg caagaaagac
     8101 gaggccagga gggcgtggca ggcggccatc aaggctcttg gctccgaccc gtccacccag
     8161 gtcatcgaac tgaaaatcga tgcgttgacc ggagcgtgac catgcgactt cccagtaccc
     8221 gccgcgccct gcgcgtagtt ctgctggcga tcggcgccgc cgcgctggcc ggctgttcgt
     8281 tgttctccag ccccgatccg cgtttcgaac ccgcgccact gacgcaatac accgccggcg
     8341 tggctttgcg ccagatctgg tcggtgtcgc tgggcagtgg gagcggctac ggttttgtgc
     8401 cagtcgtggt gggtgattcc gtctatgcgg cggcgcccag cggcaaagtg tccaagatcg
     8461 atctcgcaac cggccatgtg cagtggcagg ccagcaccaa gaagaccctg accgccggcg
     8521 tcggatccga cggcaccatc acggccgtgg ccgagatcga tggctcggtc gtcgccttca
     8581 acgatcaagg ccatgaagcc tggcatgccc aggcctccag cgaagtcaac atcccgccca
     8641 cggtgggtga tggcgtggtg gtcgtgcgtg gcagcgacta ccgtatccag gcgttcgacg
     8701 aaacgacggg caagccgttg tggaatgtgc agcgccccgg tccggcgctg gccttgaaga
     8761 ccaacatgca gatgattctg gttcaaggca tgctgatatc cggtttgccg aatggccgcc
     8821 tgatcgccat caatgcgcat accggcagcg tccaatggga agggccggtg tccatttccc
     8881 ggggcgcgac cgacctcgaa cggatcagcg acgtggtcgg cacgccgcag atacagggtc
     8941 cggcgctttg cggtgtcaca tatcaggggc atgtgtcgtg cttcgacgtg tcgcagggcg
     9001 ggcggcccat gtggagcaag gcgttttcca gcaccacggg catggccagc gacgcgcagc
     9061 aagtgtatct gtcgaaccag agcggcgtgg tgtacgcgtt cgacctgtcc gacggccatc
     9121 aggcatggaa gcagtctgcc ttgcgcaacc gtcgcctgtc ggagcccgcc gtggtccgcc
     9181 agggtgtggc cgtgggtgat ctcgctggct acgtgcacat tctgtcgcgc accgacgggc
     9241 atctgctggg gcgcatacaa gtgggcagcg atccgatcgt ctcgccgctc gtggccacca
     9301 cccatggcct ccttgtgcag tcggggtccg gcaatctcgt tttggttggt gtgaattgac
     9361 gcataccgaa ctcaagcccg tggtcgctct ggttgggcgg cccaatgtgg ggaaatccac
     9421 gctgttcaat cggctcacgc ggtcgcgggc ggcactcgtg gcgaaccagc ccggcctgac
     9481 tcgcgaccgt cactacggcg agggccgtgt gggcgatcgc ccctacatcg tggtggatac
     9541 gggcggtttc gaacctatcg ccaaggacgg catcctgctc gagatggcgc gccagaccga
     9601 acaagccatt gtcgaatccg atgtggtgat ctttctggtc gacgccaggg cgggcatcaa
     9661 cgcgcacgac catgaaatcg ccagcatgct ccggcgcagc ggccaacgca tattcctggc
     9721 ggtcaacaag gccgagggca tgcgctccgg catcgccacc gccgagttcc acgaactggg
     9781 gttgggcgag ccccacgcca tttccgcatc ccacggtgac ggtgtcgtcg atctcatcga
     9841 gcaggcgctg ggatcgttgc cgggcgccga agacgtttcg gaagtgaacg ccgtcgtcga
     9901 tggcccagag ggtgaagggc cgggcgactc cactgatgac tcggtgcgcc atcgcatcaa
     9961 gctggccatc gtgggccgac ccaacgtcgg caagtccacc ctcatcaaca ccctgctcgg
    10021 tgaagagcgc gtcatcgcct tcgacctgcc cggcaccacg cgcgacgcca tcgagattga
    10081 attcgaacgg gcgggggtgg cctacacctt gatcgatacg gccggcctgc gccgccgggg
    10141 aaaggtcttc gaggctgtcg agaaattctc ggtgatcaag accttgcagg ccatcgaggc
    10201 gtgcaacgtg gtggtgctta tggtggacgc gcgcgccgag atctccgatc aggacgccca
    10261 tatcgccggc ttcattgtcg aatccggccg cgcgctggtc gtggcgatca acaagtggga
    10321 tggcctcgat gccgaagaac gcgagcgggt gcgccgtgat ttcgaccgca agctgcactt
    10381 cctgtcgttc gccaagcatc acacgatctc ggcactgaaa gccaagggca tcaacgacct
    10441 gatccgctcg gtaggtgtgg cacacgcggc cgcgttcctg aaaatgccca caccgaagct
    10501 cacgcgcacc ctgcaggccg cggtcgagca gcagccgccg ccgcgcaagg ggatcttccg
    10561 gccgaagatg cgttacgcgc atcagggcgg gcagaacccc ccactcatca tcattcacgg
    10621 aaacgcgctg gatgcgattt cggattcgta caaacgttat ctcgaatccc ggttccgtca
    10681 ggtgttcaag cttgacggaa cgcccctgcg catcgagttc aaatcgaccc gcaatcccta
    10741 cgtgcaggag tccgcgtgaa tcagttctgg agcgaccagg tcgccggtct cacaccgtac
    10801 acccccggcg aacagcccaa gggtcaaccc atcgtcgatc tgctcaagct caacaccaac
    10861 gagaatccgt acgggccgtc gccacgcgtc atcgaggcca ttcaggcggc ggtcggagac
    10921 cggctcaaac tctaccccga ctattccgcc gccgcgctgc gcgcggccat cgccgatctg
    10981 catggccttg cgccggaaca agtgttcctg gggaacggtt cggatgaagt tctggcccac
    11041 gtgttcgacg cgttgttccg caaaccgggg cgggcattgg tcatgcccga catctcgtac
    11101 agcttctatc ggacgtattg ccggctgtac gacatcgagc cggaactcat cccgctggcc
    11161 gaggatttct cgatccgggt ggacgattat ctgcggccgc gcgcaacggt gccggccgga
    11221 atcatcttcg ccaatccgaa cgcgcccacc ggcatcgcgc tttcggtgga cgacatcgaa
    11281 cgcattctgc tggcgaatcc ggattgcccg gtggctgtcg atgaggccta cgtggatttc
    11341 ggcgcggaat cggccatccc cctgttggcc cgccacccca atctggtcgt tatccacacg
    11401 ctgtccaaat cgcgctctct cgcggggttg cgtgtcggat tcgccctggc cggggcgccc
    11461 atcatcgagg cgctgacccg ggtcaaggat agcttcaact cctacccgct cgacaccctg
    11521 gcccaggcgg gcgccgtggc cgcgatccag gacacggcct atttcgacgc gacgcgcgaa
    11581 aaggtcatcg ccgcgcgcga tgacctcgcg cgacggctgt ccggcatggg tttcgaggtg
    11641 ctgccgtccc gcacgaattt catcttcgcc cggcatccgg cccatgccgc ccaggacatc
    11701 agccaggcct tgcgcgacaa gcatatcctg gtgcgtcatt tccggcttcc acggatcgag
    11761 caatttctgc gcatcaccgt gggaactccc ggcgattgcg accgtctatg tagagttctg
    11821 gccgcgtttc tgggtacgcc ctgacgagcc caacgacctg gatgcgctgt ttggtcaatc
    11881 cgtgtttgta tgcgcgaatc aaccaagcgg cacaccaggg cgcgcgttgg cgcattgagg
    11941 gaaaaccctg taaagtacga cttcaccaag tcgtttatca aaaaacaaca cactggagcc
    12001 tagccaatga gcaataaagg gcaaacatta caagatccgt ttctgaacgc actgcgcaag
    12061 gatcacgtgc ccgtgtcaat atatctggtg aatgggatca agttgcaggg ccaggttgaa
    12121 tctttcgatc aatatgtcgt cttgttgcgc aataccgtta cccaaatggt ttacaagcac
    12181 gcgatttcca ccgtcgttcc tgcccgtccg gtcaatttcc aggcggatga ctccgccgag
    12241 tgagcaaccg gttcgttcca ccgatatcac ccgccgcgtg cgttgcgccg gcgggtgatt
    12301 cattcatggg cccgttcttc gacagcggtt cggtgggcag ggttgcatcc gcatgaaagt
    12361 gctgatcatc agcgtggatc tgggcagccc cgacttccag gcgcactccg aggagttcgc
    12421 catgctggca cgcggctccg gggctgaggt tgcgcattgc ctgagcgtgc gccgccagcg
    12481 tcccgacgcc acctacttca tcgggtccgg aaaactgcaa gaggctattc tgttggccga
    12541 tgacgctcag gtcgacgcgg tgttgttcga ccagccgctg accccggcgc aacagcgcaa
    12601 cctcgagcgt gagttcaagc gggcggtgat cgaccgggtt gggctgatcc tggatatctt
    12661 cgccctgcgg gccaagagcc gcgagggcaa gctgcaggtg gagctcgccc gtctgcaata
    12721 cctgtccagc cgtctcaccc gcatgtggag ccatctcgaa cgccagcgtg gcggtatcgg
    12781 ggcccgggga gggccgggtg agtcgcaact ggaaatggat cggcgcatca tcatcgacaa
    12841 ggtgcgtggg ctgcggacgc agctcgagcg tgtgcagcgt cagcgcgtca cgcagcgccg
    12901 ggcccgggcg cgcggcacga cgttgtcggt atccctggtg ggttatacca atgcgggaaa
    12961 atcaacgctg ttcaatgcgc tgacccgggc gggtgcctac gcggccgacc agttgttcgc
    13021 gacgctggac accaccacgc gacggctgtg gatcgatggc gctgggcagg tggtggtttc
    13081 cgataccgtg gggttcatcc gcgacctgcc gcccaccctg atcgccgcct tccgggccac
    13141 acttgaagaa accatccacg ccgatctgct gctgcatgtg gtggatgcgg cgagcccaca
    13201 gcgcgatgaa cagatcgctg aagtggacaa agtgctggcc gaaatcgggg cgggcgaaac
    13261 cccttgcata cgggtctata acaagatcga tatggcgggc tatgcgcctc gggtcgagcg
    13321 ggatgaacat ggtaccattg tgcgagtctt tgtaagcgcc ctggaacgcg atggcctgga
    13381 cggcttgcgt ggggcgattg tcgagtcagg tcgaatcgcg ggaaataatg cgtcttctat
    13441 cgaacatgtt taatttgaac gatcccggtt ggggacgtgg tgatggcaac aacggtggct
    13501 ccgagccgcc ccgtcgtcct ggcaagggtg atggcccacc cgatctcgac gaggtctggc
    13561 gtgacttcaa caaccgcctg ggttccctgt tcggacgcaa accacgccgc ggcggtggct
    13621 ttggcggtaa cgggggcggc ggcacgccgg tcatgctgcc caaggggtcg cccaagcttg
    13681 cggccctggt cattctcgtc atcatcgtgt tgtggctggc gagcggcttt ctcattgtgc
    13741 aggaagggca ggtggccgtg gtcacccgtt tcggcaagta caccaagacg ctgcctccgg
    13801 gtttgcagtg gcgtctgccg tatccaattg aagactcgca gatggtcaat atatccagac
    13861 tgcgcacatt cgagatcggc tttcgagggg gtgcgaccaa caaggttctg ccggaagcgc
    13921 tcatgctcac caatgacgag aacatcgtcg acgtgcagtt cgtcgtgcag tatcgcctga
    13981 agccaggcgg tgcgcccgac tacctgttcc gcaccagcca tcccgacgaa gccgtgcgcc
    14041 aggcggccga gaccgccatg cgtgaggtgg tcggcaagaa accgatggat cacatcctgt
    14101 attcggggcg tgccgaggtg gccgccgccg tgcagaagcg tatccagagc ctgctggacg
    14161 aataccagac cggtatcgag gtcagcaccg tggccatcca gaacgtccag ccaccggagc
    14221 aggtgcaggc ggcgttcgat gacgcggtca aggccggcca agaccgcgag cgccaggtca
    14281 acgaggggac ggcctacgcc aagaaggtca ttcccgaggc tgacggcgag gccgcgcgga
    14341 tgatccagca ggcggaaggt tacaaggcca aggtcatcgg cgacgcccaa ggcaacaccg
    14401 cgcgtttcct cagcatccag tccgaatacg ccaaggcccc cgagatcacg cgtgagcgcc
    14461 tgtatctgtc gaccatgcag gacattttcc agcacaccag caaggtgatg atcgacgcca
    14521 acgcgtcgaa caacctgctt tatctaccgc tggatcggct catgaaagaa gccgccaacg
    14581 aggccgcgac gcaatccgcc gccgcttccg ccgtggcgac ggatgcttcc tcgacgtcct
    14641 cgtcagggtc ctcggcctcg cccagcaagg gctcgcggaa atccgcctcc gacacggcgc
    14701 gccatgattc atcgctgccc agcgttcccg attccttgcg ggacacgctg tcgctggacc
    14761 gattctcgca ttgatgaaag gggctaccat gcaacgtttt ttccccgccc tgatcggact
    14821 gatcgttctt gtcggaattc tgtcgtcgtg cgtgttcacg gtcggtgagc gcgacgatgc
    14881 gttggtgttt tcgctcggtg aggtgcgcaa gaccatcacc gagccagggc tgtatttcaa
    14941 attaccgcct cctttcgaaa acgtggtgct gctcgacaag cgtctgcaga ccatcgagac
    15001 caatcagccc gaacgcatcc agacgtccga gaaaaagaac ctgctgatcg attccttcgt
    15061 gaagtggcgc atctccaatc cgcgcctgtt ctacgtgacg ttcggctcca acacccgtgc
    15121 cgcgcaggaa cgcctgcagg cgcagattcg tgacgccttg aacgcctcgg tcaacgtgcg
    15181 taccgtcaag gacgtgatcg ccaatcagcg cgacgccatc ttgaaagaag tgcttggggc
    15241 ggttcagagg cgcgccaagc cgctgggggt ggatgtggtc gatgtgcgcc tgcgccgcat
    15301 cgagttcgcg cctgagattt cggaatccgt ctatcgccgc atggaggcag aacgcaagca
    15361 ggaagccaac cgcctgcgtg ccaccggtgc cgccgacagc gagaaaatcc gtgccgaggc
    15421 tgaccgccaa cgccaggaaa tcctggccaa ggcttatgcc aaggcccaga ccatcaaggg
    15481 cgagggcgat gccgacgcgt cctccattta tgccaaggcg tattccaagg atccgaagtt
    15541 ctacagcttc tacaaaagtc tgctggccta tcgcgacgcg ttctccaagc ccaccgacct
    15601 gttggtgctg agcccgaaat cggagttctt ccggttctgg aactcatcca cgggcggcgc
    15661 gacagggggc ggagcgctca aggccggcgc ggcccattga ggtggttgtc gcatttatcc
    15721 acccgcaaaa tggcgtaacg gaataaatcg gctatactac tttgtaagtt tttagatggg
    15781 tcatgcgccc gtcacgaccc ggctcgtgcc gggttgtggc tttagcgggt ttcgcgacgc
    15841 acgacaccgc cttcgcaagc gacttggcgg gcttacgccc caagtcgctt ttttcttgtt
    15901 catcaaacct gtcgacctct attcattggt cttgtccgga tcgccaagat gtccaactgg
    15961 ctgttgcctg aaaacctggc tgatgtgctg cccgccgagg cgcgccgcat cgaggaattg
    16021 cgacgcgaat tgctcgactt gtaccgtacg tacggctacg agctggttgc gccgcccctg
    16081 gccgaatacc tggattccct gctgtctggc gctggccatg acctgaacct gcgcacctgc
    16141 aagctggtcg atcagctttc gggccgcacc cttggcgtcc gggccgacat gaccccccag
    16201 gtttcgcgca tcgacgcgca cctgctcaat cgggaggggg tggcacggct ttgctattgt
    16261 ggttcggtgc tgcacgcccg cccggccggc ttgctgtccg agcgcgagtt gctgcagatc
    16321 ggcgccgagc tgtttggcca tgccggggtc gaggccgata tcgaaatcat ccagcttgcg
    16381 ctggaaagcg tgggccgtgc cggcatcggg catgcccggc tcgacttgaa tcatccaggt
    16441 atcgtgcatg ccattgtgcg cgccgatccg gcgctcgagg gcgtggaaga agatgttttc
    16501 cagctgttgt gcgccaaaga tcgccccggg ctggtcgaac tcggccagcg gttgcccggg
    16561 cttcgcgccg atagcctcga tgcactgtgc gcactgacct ctctctacgg ggggaccgaa
    16621 gtcctcgacg aggcatggcg ggtgctgccg ccgttgccga ctttgcgcgc ggccctgggc
    16681 gatttgcgca cgttggtcgc ggcgctgccg catcacgagt tcagtgtcga tctggccgat
    16741 ctgggcagtg gctatgggta tcattcgggc gtggtgtttt ccgtgtacgc ccagggctgg
    16801 cacgacgcgc ttgtgaaagg cgggcgctac gatggcatgg gtaaggcgtt tggccgggca
    16861 cgcccggcca ccgggttcag tcttgatttg cgtaaattgt cggccggcct gcccacggcg
    16921 ccgttgtccc gcgcgatccg ggccccgtgg ggtacggcgc ccgatctgct ggaggcggtg
    16981 cgcgccttgc gtggcgccgg cgaggtcgtc gtgcaggctc tgcctggcca ggcgtaccag
    17041 atggatgagt tcagcgtcga tcgtgaattg gtcgtcgaag acggtgtgtg gagaacaagg
    17101 gcgatcgaat aaccgcgtgg catgcggtgc atcgcctgat ctttaatcct attggcccgc
    17161 tgtaccgggg ccgatgcttt tcgatatgcg acatgagcaa gaacattgta atcatcggca
    17221 cccagtgggg tgacgaaggc aaaggcaaga tcgtcgactg gctggccgaa tcggcacagg
    17281 gcgtcgttcg tttccagggt ggccacaacg ccggccacac cttgtggatc aacggtaaga
    17341 aaaccattct tcggttgatt ccatcgggca tcatgcaccc caatgtgacc tgctatatcg
    17401 ggaatggggt cgtcttgtcg cccgaggcgc tgttgaccga aatcgccgaa ctcgagcagg
    17461 cggggctcga agtgcgctcc cgcctgcaga tctcgcccgc ctgcccgctg attctgccgt
    17521 atcacgtggc gctcgatcag gcgcgcgagg cgcgcaaagg ccccggcaag atcgggacga
    17581 ccggccgggg catcgggccg gcctacgaag acaaggtcgc gcgccgggcg ctgcgcctgc
    17641 aggatctcta cgatcccgcg attttcgatg aaaaactggc cgaggtgctg gactatcaca
    17701 acttcgtgct gaccaactac ctgggtggga aggccctcag cgccaccgag gtgcgcgata
    17761 aggtcatggc gttggcgccc cgtatcgagc ccatggtggc cgatgtctcg aacaatctga
    17821 gcattgccca gaaagcgggc cacaatctcc tgttcgaagg cgcccagggg gcgttgctcg
    17881 atgtggatca cggcacgtat ccgtatgtca ccagcagcaa ctgcgttgcc ggtgccgcag
    17941 ccgccggcgc cggcgtgggg ccgcaatcgc tgcattacgt gctcggtatc gccaaggcgt
    18001 acaccacgcg tgttggctct gggccgttcc ccaccgagct cctggatgat accggtgccc
    18061 gcctggccac ggtcggcaag gagttcggtt cggtaaccgg tcgcccgcgc cgttgcggtt
    18121 ggttcgacgg ggctgccatg aagcgttccg tggccatcaa cggtatctcg gggctgtgca
    18181 tcaccaagct cgacgtgctg gacggcgtcg aagaaatcaa aatcggcgtg ggctatcgct
    18241 acaaaggcca gttcctcgac gttctgccct atggcgcgca cgccgccgcc gaggctgaac
    18301 ccgtcctgga ggaaatggcc gggtggcccg agtcaacggt gggtatcacc gaatacgaca
    18361 agctgcctgt gaacgcccgc cgctacctcg aacgcatctc cgacgtctgc gaggtgccca
    18421 tcgacatggt gtccacggga ccggatcgcc tcgaaaccat cgtgttgcac catcccttca
    18481 acggataggc agggtggggt gtgacacccc gggtgttgcg tccaatccat gggttttcgg
    18541 tgaaaacggc gaaatccggc cggatttcgc ctcttggtct ttgcgccgag gcttgcgggg
    18601 tttactatta cgaaccaatc cccctggctg taacattttt ccaacgcccc ctgatggggc
    18661 gacaagtgtt tgttccagcc tgggtggggc cgtattcgta ttctttaccg cgttcatatt
    18721 gtcgaaggag cctatcgcat gagcctgccg cagagcaccg accgggacct ctgggttagt
    18781 tgggacgact atcacagcct gatcgaacgc ctggcctcga acgtgcacga atccggttgg
    18841 cagttcgaca aaatactttg cctggcgcgc gggggcatgc gtgtcggtga tgtgatgtcg
    18901 cgcattttcg atgtcccgct cggaatcctg gccaccagca gttaccgcga agcggccggc
    18961 acattgcagg gccagctcga tatcgcccag ttcatcacca ttacccgggg caccctcgat
    19021 ggccgggtcc tgatggtcga cgatatggtc gacacgggtc tgacgttcaa tcgcgtgcat
    19081 caacatctgc tgtcccagtt tcccgccatc tccgagatgc gcagcgccgt attgtggtgg
    19141 aaagggcacg cccaggtgca tcctgattat ttcgtggaaa agctgcccac caatccctgg
    19201 atccatcagc ccttcgaaga ctacgacagc ctccggccgc atcaactcga ggcctgggtg
    19261 cggaaagggg tccgcggcta gcggtgggat tgtgtttgcc gggatgtgtc gcgcctgccg
    19321 cttgcggtag gtgtttcatg ctaaaatcta gagttactgt tttatttcgc ttactttgtt
    19381 ggcccatttg ggttggccta atctggcttt ttggatacta catgcctatt gtccgtctga
    19441 aagaaaacga gccctttgaa gccgcgcttc gccgcttcaa gcgcaccatc gagaaaaacg
    19501 gtctgctgac tgaactgcgc tcgcgcgaat tctatgaaaa gcccacggct gaacgcaagc
    19561 gcaagcatgc cgcggccgtc aagcgccact acaagcgtat tcgcagccag caattgcctc
    19621 cgcgcctgta ttgatccctg actcttttgt ccaggatcta cttgcccggg tcgatgtcac
    19681 cgacgtagtc gggcggtatg tgccgttacg caaggcaggg gtcaatttgc ttggcctttg
    19741 cccgttccat aacgaaaaaa gcccatcatt taccgttagc cccacaaagc agttctatca
    19801 ttgctttggg tgtggtgcgc acggcacagc catttccttc cttattgagc acaccggtgc
    19861 cagcttcccg gaggcggtga gggcgctcgc ggccaccgtc ggcatgacgg ttcccgaaga
    19921 ggcgcgcagc cccaggcagc aggcggcatc gcgccagcgc aaggaagagg cgtcacgcca
    19981 ccagaaagcg ttggagcagg cgcagacgca ttacctgaac gaactcaagg cctcggcggc
    20041 tgccatcggg tatctgaaga agcgggggct gtccggccag accgcggcgc gtttcggcct
    20101 gggttggtcc ggcaccgaca ggcgcggcct ggcaaaggta ttcgagcatt acgaagaccc
    20161 gaccctggtg gaatcggggc tggtcattga atccgaggat ggccgccgtt acgaccggtt
    20221 ccgggaacgg atcatgtttc ccatccggga cgcgcgtggc cggctgattg gcttcggcgg
    20281 ccggatcatc ggcaagggcg agcccaaata cctgaattca ccggaaacat ccctgttctc
    20341 caagggccaa gagctttacg gcttatggga ggcgcgcgcg gccattcgca gcgaggggca
    20401 cgtcctggtg gtggaaggtt acatggacgt ggtcagcctc gcgcatcagg gcgtcgagaa
    20461 cgcggtggcc acgctgggca ccgcgaccac cgccatgcat gtgcagaagc tgctgcgcgc
    20521 gagcgaccgc atcatcttca gtttcgatgg ggaccaggcc gggcgccgcg ccgcgtggcg
    20581 cgccctgcaa caatgcctgt catcgctgcg cgatgacatt tcggtgcggt tcctgttcct
    20641 gccgccggag catgatccag actcgtacat acaagcgttc ggccaagagg cgtttcgcgc
    20701 cacggtcagt gaagccgcgc ctttatcgcg attcctgctc gacgagctgg cttcgcgcca
    20761 ccagatggat gaggccgagg ggcgggccgc gtgtgtgcac gaggcgaagc cgctgctggc
    20821 gcaattgccg cagggagcat tgcggttaca aatagaacgc gagttcgcgc gcctggtgcg
    20881 gctcacgcca gaggaactgt cgtcgatgct cgatgcgata ccggccacgc ccggcgattc
    20941 gccgtcttcc ggcggccagg cagcctcatc caggccgata cggtcgggcc aggccgtgtc
    21001 gcaggacgag cctgaagggt tcttcgcgga ttcttctccg gtggacgact tcccgccgga
    21061 tgtcttcccc atgggcgcca tctccacctc ccgcacgcgt cggccaagac gcgcgccggc
    21121 ccggatggtg acgcccatgg cgaaacgcct gctgcgcctg ctggtggctc acccagggct
    21181 ggtggcctcg ctgggtgatc aacagcttga aattgtctgc cgcagccccc atcttgtact
    21241 tgtgcgggac ctgatagcgc tgatcaacgg cagcggctcg cgccatgttg gtgccttgct
    21301 gcaggccgcc gagcccggct ccgatctcga agaggtcttg ttgtccctgt cggcggagct
    21361 cctggcgcag gacgatttgc ccgacccgga tgccgaatgg aacgatgcgc ttgcccgcat
    21421 tgagctcgac gcggtcaagg ccgaacagtc ccggctgatc gagaccgggc tgaaggatga
    21481 cgccgcgcgc cggcgctatc aagagttgac gcgccgcatc gcactgctgt cgggtgcgtc
    21541 tttacgttga ctactgtaaa atgattagtt tacgcaagct attgaatggc ttgtgatttt
    21601 gacggaatgt gcagtgggcc ccagacccat cggctctttt tgccctgatg tgcagccaaa
    21661 gcggcagcca aagccaggga ttacgaaccg acgagcgatg cgtccagtgc gacacttgta
    21721 catgccacca gtctgtgatg aacgatttct gcccgacggc acctgccggc ccttgagccg
    21781 ccgggtgctg ttgagcctat ggaagcgact atgacccaag ccactggtaa gaactccaaa
    21841 gcaacaaaga acaccgacga cgacaagaag aaggtcgcgg cggcgtcccg ctccgtcgac
    21901 aagacgacgg ccacggcagg ggagtccaag gccgggaaac ccgcggccac gaaggcggcc
    21961 cgcaagcccg agggcgctga cgcgaagaag gccggcaagg gcgcggccaa ggccgcatcc
    22021 cgtcccgtgc ccagcggccg ccgccccggt cgcccggcca agaacgccaa caacgatgcg
    22081 gcgaacttca gcgacgacga tgtcgaaccc gaagtcatcc cagaactcaa accgctgccc
    22141 aagcgcggag gcaaacgcgg caagggcgac aaagacgtcc cggcccgcag ccaaatgacg
    22201 ccggaagagc aagaggcgcg ccgcaaccgc ctgaaagtcc tgatcaagct gggtaaggag
    22261 cggggctatc tcacctatgg tgaaatcaac gatcacctgc ccgacgacct ggtcgacgcc
    22321 gaggccatcg acggcatcat cagcacgttc agcgacatgg gtatctcggt ctacgaccag
    22381 gcgcccgacg ccgaaacgtt gctgatgagc gacaacgcgc cggtcgccag cagcgacgac
    22441 gacgtcgaag acgaggccga ggcagccctg accaccgtcg actccgactt cggccgcacc
    22501 accgatccgg tgcgtatgta catgcgcgag atgggcacgg tcgaactgct cacgcgcgaa
    22561 ggcgagatcg aaatcgccaa gcgcatcgaa gacgggctca agcacatggt catggccatc
    22621 gcggcctgcc cgacgacggt caatgaaatc ctggcgcatg tccagcgtgt gcgcgaagga
    22681 accgcccaga tcgacgagat cgtcgatggt ctcatcgacg aagagggcga ggaatacgcc
    22741 ggcgccgggg tgtcttccga cagtgacgaa aacgaagatg ggcccgccgg cggcatgagc
    22801 agcaagcaac tcgagaatct gcgtgtcaat tcactgcgca agttcgagct catcggccag
    22861 tggttcgaga aaatgcgcaa ggccttcgaa accgaaggat actgctccga cgcctacgtg
    22921 cagtcgcagc aggagattct cgacgaactc atgggcattc gcttcaccgc gaagatggtc
    22981 gagcggcttg ccgacacgct gcgggcgcag gtggccgaag tgcgcaagtt cgaacgtgaa
    23041 gtcctgcata cctgcgtcga ccgcgccggt atgccgcgag cctatttcat caaggttttc
    23101 ccgggtaacg aaaccaacct gaactgggtg ctcgaagagg tcgcggccag ccatccgtac
    23161 tccgaaacgc tcgagcgcca cgtaccggcg gtgcaggaaa tccagcagaa gctcatcgat
    23221 ctgcaagcca gtgtcgtgtt gccgctgaaa gacctgaaag acgtgaacaa gcgcatggcc
    23281 acgggcgaag ccaaggcgcg caaggccaag cgtgaaatga ccgaggccaa tctgcgtctg
    23341 gtcatttcca tcgccaagaa gtacaccaat cgcggattgc aattcctgga tcttatccag
    23401 gagggcaaca tcgggttgat gaaggcggtc gacaagttcg aatatcgccg cggctacaag
    23461 ttctcgacct acgccacgtg gtggatccgt caggccatca cgcgttccat cgccgatcag
    23521 gcgcgcacca tccgtattcc ggtgcacatg atcgagacca tcaacaagat gaatcgcatc
    23581 aaccgccaga ttctccagga aaccggtgcg gaacccgatc cggccacctt ggcgcagaag
    23641 atggacatgc cggaagacaa ggtgcgcaag atcctgaaga tcgccaagga gcccatctcc
    23701 atggaaaccc ccatcggcga cgacgacgat tcgcatctgg gggatttcat cgaggacagc
    23761 ggcacgcttt ccccgtccga gtacgccctg cacggctcca tgcgcgatgt ggtcaaagaa
    23821 gtgctggatt cactcacgcc acgcgaagcc aaggttctgc gcatgcgctt tggtgtggaa
    23881 atgagcaccg accaaacgtt ggaggaggtc ggcaagcagt ttgacgtgac gcgtgagcgc
    23941 attcgccaga tcgaagccaa ggccttgcgc aagctgcgtc accccagtcg cgccgacaaa
    24001 ctcaagagct tcctggaagg tcagtgaata ggcggcctat ggccgcgata tactgacaac
    24061 gccagatgcc gggcatagcc gctccggctg cgggcccagg tacacgccgc cgcgtgtgcc
    24121 tgggcctttc tcattacagg aatggccgaa tcggtacaat attccgagga tggttttttg
    24181 gtgagccatg gttggcatgg cgccgatatt cgggtgattc catccaacga acctccaact
    24241 tggggctcca gtcgagcata tccatgattt cgccgcttct atccgacccc gtcgtcgaac
    24301 ccgagggcga ccgctgcctc atgttgcgct atggaacgca gctcgacgcg cgggtttccg
    24361 cacgttgcct ggccaccgcg cagttgatcg aacatgccaa cctgccaggc gtgttcgacg
    24421 tcgtgccatc gttcaccact gtggccgtgc attaccagcc gtcggctttt ccacgggacc
    24481 cctacgagac attggcggcg gcggttctcg cgttgctcga acccgcggcg gcggcgcccg
    24541 ggttcgagcc ccgaacgatt cgagtgcccg tctgctatga cgatgtattc gcgatggacc
    24601 tggagtatgt gtccgatact tgccacctga cgaaacaaga ggtcataagc ctgcacacgt
    24661 ccgaccgcgt cagcgtgttc gcgctggggt tcgcgcccgg catgccatac atcggcgtgc
    24721 accatggaaa actcgatctg ccccgccgcg ccacgccccg caccgacgtg ccgggagggt
    24781 cggtcgccat cgcgaacagg caaagtgtga tctatcccta caccagcccc gggggatggc
    24841 atgtgatcgg tgccatgccc atgccgttgt tcgaccccgg caagccgcca tactcgttcc
    24901 tgttgcccgg ggacatcgtg cgattcacgc ccgtttcccg cagcgagttc gagacactgc
    24961 gccaggacca ctcatgagca tcgaagtcat caaggcgggg gctctgtcgt tgttccagga
    25021 caatggccgc ctcgggttcc agaagctggg ggtgccggtt tgcggcccga tggacgaaag
    25081 cgcgcaccgg ctggccaatc tcctggtggg caacggcggc gatgcggcgg tgttggaaat
    25141 caccttgatg gggccgaaac tgcgattcac ggcgcccgct ggggtcgcgt tgaccggggc
    25201 ggacctgggt gcccgcgtgg acggccttcc gcttgcgcag aatcggccgc tcatcgtgcg
    25261 tgcggggcag gtcctggatt tcacaggcgg gaaatccggc atgcgcggct atctggcggt
    25321 gcatggtgga ttcaacctcg aaccccaatt gcgcagttgc agcacgtact tgaagggaca
    25381 cctgggaggg gtggacggcc gcgcactcca acccggggac gtcatcggat tgaggtcggc
    25441 cctgtcggtc gatcccgatt tccttcagga ggccgagcgc cggctttggg aagtccagtt
    25501 gtacctgtcc gcccggctgg gactggtccg gcgtgacacg ctgcgcgtag tcaaagggcc
    25561 gcactggggg ctgttctccg agaagtcggt ccatgatttc gtgacggccg gcttccgtat
    25621 ctcgccgcaa tccgatcgca tgggatatcg catcgccgga cccggcctcg ccgtgaagaa
    25681 accggcccag atcttgtcgg aagccacggc tttcggcgcc gtgcaggtgc cccatggcgg
    25741 ggatcccatc gtgatcatgg ccgatcgcca gaccatgggc ggctatccca agatcgccta
    25801 tgtcgcctcg gtcgatctcc cgtcgctggc gcagagccgg ccaggcgagg tggtgcgatt
    25861 caacctggtc tcgctggagc aggcgcaggc gctcgccaag gcccgcgacg ccgctttcgc
    25921 tgaactgggc gcgcgtctgg cgtgcgtaag tggaattcta tccggaggtt caccatgaca
    25981 ctatccatcg acctcaattg cgatatgggc gagagctttg gcgcctggaa aatggggcac
    26041 gacgaggccg tgctgccctt cgtgacctcg gccaacatcg cctgcggctt tcatgcgggt
    26101 gatccggcga ccatgcgcaa gacggtgtgc gcggcgctgg agcacaaggt cgcgctgggg
    26161 gcgcatccgg gtctgccgga tctggtcggg ttcgggcggc ggaacatgga tctctcgccc
    26221 cagaacgcct atgacatcgt cgtcgtccag gtcggcgcgc tggctggcgt ggcggccagc
    26281 cagggtgcgc ggctcaatca cgtcaaggcg catggcgcgt tgtacaacat ggcggccagg
    26341 aacaaggatc ttgcccgcgc gctggcgcag gccgtgcatg acgtggatcg ggatctgatc
    26401 ttctacgcgc tggcttccag cgccatggtg gaggccgcac atgaagtcgg cctgcgggtc
    26461 gcccaagagg tcttcgccga tcgaacctac cagggcgatg gctcgctgac accgcgctcc
    26521 cggcccgagg cgatgatcgt cgattcccag cagtccattg cgcaggtgct gcgcatgatc
    26581 cgcgagggta aagtgaggtc cgtgcagggc accgatatcg ccgtgcgggc cgataccttg
    26641 tgtatccatg gcgaccaacc cggagcggtg atgttcgcca agtccattcg tgcagcgttg
    26701 caggcggaag gcattgaagt gcggacggtc ggccagccat gacggcgttc gattccggat
    26761 tcaccggtcc ggactgttcc atccgcacct acgatggcga ggcgcacacg catgcgcacg
    26821 gttatgcgca gatcatggtg ccgctggccg ggcgcatgga gctggaaatc gcgggccgcg
    26881 cgctgttcac ggacatgtcc tgcggcatgg tggtgccggc gggggcctcg catgcctacg
    26941 tggcacggcc cggcacccgc atgctggtta tcgacacgcc cgagcagaag gggttggaac
    27001 gggtgcggcg cttcgccatc acggcggtct gcgcctcctt ggccgacatc ggcgacgtgc
    27061 agaggcagtt gtcgctcgtc ctggaactgc ccacggtgtt gtcgcgccgg gggctcgatc
    27121 tcgcccgact ggacgccgcg ctggatgcgg ggttgcacga agactggcct gtttcccgta
    27181 tggccggact attcttcctg agcgcacagc ggttccacgc gcgcctgctc gaactcaccg
    27241 ggcgcacacc agccgcctac ctgcgcgacc gtcggctcga tgccgctgaa aaggcgctgg
    27301 ccaggggggc gaacctggag gccgtggcgc agcgggtggg gtaccgcacg gccagtgcgc
    27361 tggcttttgc gcttagccgg gaccgccatg ttggcgcccg ctcattgcgc cgccgtggcg
    27421 tcgtgtaggc caggcgtggt catcgcgcga cggtccgata tgccttcaac gcggggcgcc
    27481 gccgccctga agaagagaga ccgtcatgga tcgggacgta gtcccgccgc gagatcgaga
    27541 gtttctcgat agttggatct ggcggtcatg tcacgatggc ggtatgaaat cgtcgtcgac
    27601 aacaggaatc atactggtgc tgtgcgccgc cgtgctctgg ggcacgaccg gcacgacaca
    27661 gagtttcgcc tccagcgcgt tgtcacccta ttgggtgggc gcgttgcggc ttggcatcgc
    27721 ctcgctgttc ttttgtgtct atgcgggggg caggctgggc ggacgacggt tggcgcgcga
    27781 actcttgggg ataccgtggt tatgggctgt gctggcgggt gcctgcatgg cgggttacaa
    27841 cctggcgttc ttcgcgggtg tgcgggccac gggcgtcgcg gtgggcacgg cgctggcgct
    27901 gggcagcggg ccggtgtggg cggggttcct gcaaatggtg gtcgcccgca gggcgccagg
    27961 ctccggatgg tggctgggca cgatgctggc cgtcgcgggc ggcattctca tggtgttggg
    28021 gcgtggcggg gatttgcggg cggatgccac gggtatcacg ctgtgcctgg tgtcggggtt
    28081 gtcctatgcc atctatgccc tggtcagcaa gcacctggtg ggccggaacg atcccggcgc
    28141 ggtcacgctg gtggtcttcg gcgtgggggc cttgatcgcc atgccggtcg cgtgggcctg
    28201 ggtgggtggg cccgacatcg ccggacgtgg gtgggcggtg gttgttttcc tgggcgtggc
    28261 cgccacgggc atcgcctatc tgttgttcaa cacgggcttg cgcggcatcg cggcggccag
    28321 cgcggtgtcg ctggcgctca tggagccgct gacggctttc ctgctggcga tagtcgtcgt
    28381 gggtgaacgt ccggcggcgt cggccttttt cggtctcgtg tgcgtgctcg ccggcctggc
    28441 cctggtcata cgggccgaga tgcgccgtca gccctgagcg cgcaactgct tgcggatgat
    28501 cttgcccgtg gccgtcatgg gcagcgactg cacgaaactg acctgacgcg gatattcatg
    28561 ggccgctgcc ttgcggcgca cgtgatcctg gatctcggcc accagatcgt cactggggga
    28621 atagccgtcg ttcagaatga tgaacgcctt gacgacttcg gtgcgctgcg tgtccggcac
    28681 gccgaccacg gcggccattt tgacggccgg atggctcaat aggcagtctt cgatgggccc
    28741 tggaccgatg cggtatcccg cgctggtgat gacgtcatca ttgcggccca cgaactggat
    28801 gaagccgttc gcatcgcgta tgccgcgatc gccggtgatg agataaggcc cggcgaattt
    28861 ttcgcgtgtg gcttccgggt tcttccagta gttcaggaac atgaccggat cgggtgcccg
    28921 cacgccgatg tcgccttcgg tgccgtccgc cacgggatgg ccgtggtcgt cgacgatggc
    28981 gagatcatgg ccgggcaccg cacggcccat ggtgccgatg agtgggtcga acagcgccga
    29041 gcatgatgag acgatcatgt tgcattccgt ctgtccatag aactcgttga tcgtcaggcc
    29101 aagatcgcgg cgcccccatt cgatgagctc aagccccagt gattcgccgc cgctggcgac
    29161 cgataccagt ttcaatggcc agcgcgcgcc ggggttggcc accgtgcgca gcattttcag
    29221 tgcggtgggc ggcaggaacg tgtgggtgac gccgtggcgc gacatcaggt cgtacgcggt
    29281 ttcagcgtcg aatttcgcga agcggcgggc cagcaccggt acgccatggt gccaggaggg
    29341 cagcagcacg tcgagcaggc ctccgatcca ggcccagtcg gcgggcgtcc acatcagcgc
    29401 cgcgttcttg ggaaagaagt tgtgcgacat ctcgacgccg ggcaggtgcc ccagcagcac
    29461 gcgatgcgcg tgcagggctc ccttgggctt gcccgtggta ccggaggtgt agatgatgac
    29521 cgccgggtcc tcggccaggc tatcggccgt gaagggttcg ccggactggg cttcgagttc
    29581 gggccagaaa ggtagccagc cggcttcttc gcagtcggcg tcgatgtcga acgtggtgac
    29641 gaggtcgggc aatgaagagc gaacctcggc cagcttgcgc cgaccctcgg cgtgggtgat
    29701 cagcgccgcg gcgcccgagt tgcccaggcg gaactgcagg gcttcggtgc cgaacaaagt
    29761 gaacagcggc acggcgatgg caccgatctt gtagatggcc aggtgcgtga tggcggtttc
    29821 caccgcctgc ggcaggaaaa tgccgacgcg gtcacctttt ttgacgccat gcgccagcag
    29881 cgcgcgcgcg aaccgatcgg acaatcgctt catgtcgtcg aaggtatagc gcgactgttc
    29941 acccgtgggc ttttcgtaga tcagggccaa ccgcccggaa ccgtccgccc acttgtcgca
    30001 agcatccacg ccgatgttgt agtgttctgg aacctcccac ttgaaagcct tgtacgtggc
    30061 cggataatcg ttttgcgcaa ccagcatttt gtcgtcctga gtgatcttgt cattgccctg
    30121 ggccgcgctg tgcaacgctg atgtcgtctc cgacccgcga tacggatgat acggataggg
    30181 ggtgcgcccg gcgctacata tgctagcacc gaaccctgta tgcggtgcgg aaggaaggtc
    30241 gatgcggatg gccgcacccc gcatcgtttc ccgtgtgtgg catgcaacag ggctcgcctc
    30301 cattgcgaag gcgagccctg ttgcgctgat gtcggggcga ggcgtcagtc gagtagactg
    30361 gcgtcccagg cgtgcacggt ctggcgctgc tcgcccaggc catcgatgcc caggtgcacg
    30421 acgtcgcccg ccttgaggta caggggcggc ttctggccga ggcccacgcc gggaggcgtg
    30481 ccggtggaga tcaggtcgcc cggattgagc gtggtgaatt ggctgatgta gtggaccaga
    30541 tgggcgaccc cgaaaatcat ggtgctggtg ttcccgttct ggaagcgctt gccactgacg
    30601 tcgagccaca tgcccagttt ctgcgggtcg gggatttcat cggcggtcac cagccacgga
    30661 cccaccggac cgaaggtgtc gcaacctttg cctttgtccc aggtgccgcc gagctcgagc
    30721 tggaattcgc gctcggacac gtcgttgacg acacagtagc cggccacgta atccagtgcc
    30781 tcgtccacgc cgacatagcg ggccttgcgg ccgatgacca cgcccagttc gacttcccag
    30841 tcggttttga ccgacccctt gggcatgacg atggggtcgt tgggcccggt ggcggcggac
    30901 cacttgttga acaggatggg ttcagagggg atggctgctc cggtttcagc ggcgtgatca
    30961 ctgtagttca ggcccacgca gatgaatttg ccgacaccgg catagggcac gccgagacgg
    31021 ccgggatttg tgatgacggg caggctgttg acgtcgaccg cgcgcagggc gtcgagttgt
    31081 tcgggggcga gcgtgctagc tgaaatgtcg gggatgacgc ccgaaagatc gcgcagtttg
    31141 ccctgggcgt ccaggcaggc gggtttttct tggccgacag ggccgtggcg cagcagtttc
    31201 atgagactgt tttcctgttc cgggttagtt ggaccagcca ccgtcgatga cttgcgacgt
    31261 gccggtggtg aaggaggatt cgtcggacgc gaggtacacg gccagcgccg cgatttcttg
    31321 cgggcgaccg acacgaccaa tgggttggcg cgcgacgaaa tcatcgtgaa tctgcttttc
    31381 agacttgccg tatttggcgg cctgcgaggc gatgcgttca cgcagtgacg gggattctat
    31441 ggtgcccggg cagatggcgt tgcagcgcac accctgggag acgtagtcgg cggcgatggc
    31501 cttggtcatc ccgatgacgg cggccttggt ggtgccgtag acgaaccggt tgggcacgcc
    31561 cttgatactg gaggcggccg aggacatgtt gatgatggag ccgcgtttct gttggagcat
    31621 gccgggcagg aaggcgcgga tcatgtggta catggaggtg acgttcagtt cgagagagaa
    31681 cgaccaggca tcgggttcgc agccaaggat gttgccatcg tggacgtagc cggcacagtt
    31741 gaagagaacg tccacgctgc ccaattcgtg cgccagcgtg gcgatctggt cggggcgggt
    31801 gacgtcgagc acacgggttt cgcagtcttg tatgtgggcg agcgtgtgcg ggttgatatc
    31861 ggtggcgatg acgcgggcgc cttccgccgc gaacgcttcg gcggtcgccc ggccaatgcc
    31921 ttgtcccgcg gcggtaacca ccgcggtttt tcctgtcaga cggttcgtca tgtcgtgctc
    31981 cggaatcgaa tcgagtgggc cggcactgtg gcggatgcca caagcaagct ggttttattc
    32041 gtgaaattta attttctacg ctgtgttgtg catcctaaac cagtattcca ctctgatcaa
    32101 tcccgaattt tccttatatc atcggaaata taactttccg gtgggcgaga ttggaggctt
    32161 tctggatggt gatgatcgca ccaatttttg tcgaattcga gaaaatatat ttttatatat
    32221 aaatatatat ttcgtttgtg aaaaatatga gtaataatgc cagtgttcgg cttgatgggt
    32281 ccggatattc aaattcgaat gggtgtggtc aatcaaacca actcatcctt ccgtcggggg
    32341 gcgagtcgtg cgcaccacac aaaaagagca tctcccatac aaaaaggatc atctcatgaa
    32401 acgtgtttcc gcgacgacaa agctggttcg ggctggcgat ggatcctcgg tacagagcga
    32461 ttccgcatcg cgcggcatgg ccgacgcaca gaatggggac tccgacgcaa agaacggggg
    32521 ccctggcgca agcgccggca ccacacgcta cgccgcgccg gcgttggaaa agggcctgga
    32581 catcctcgag gcgctgagtg tcagcggcga ggggtacacg ctcaatgaac tgtgccagac
    32641 gcttgggcgc aacgcgagtg agctgttccg catggtggtc acgctgcagc ggcgcggcta
    32701 catcgaggct gggccgggcg atcgctacac attgacactc aaaatgttcc agctcgcgca
    32761 ccgccaaccg tcgttgcggt ctttgaccga tgtcgcgcta cccttgttgc gtgaattgtc
    32821 ggagcgcgcg cgccagtcct gccacctgtc gatcttccag gatggacggg tggtgatcat
    32881 cgcccaggtc gacagcccgg aacgctggtc gttcgggttg aaagtggggg tggccatggg
    32941 gttggtggac acctcgtccg ggcacgtgct gctggcgtcg cagaacgagg tcgagcgtgc
    33001 gcgcatgctg ggtggccatg tccgggtcga gggcgaattg gccatggacc ccgggcagct
    33061 cttccccctg ctcgatgcgg ctaggaccaa cggttatgcc tccatgccca gcgtgcagat
    33121 ccagggggtc accaacatcg cgttccccat ccgcggtccg ggcgggcggg tggtcgcggc
    33181 cgtgaacgtg ccgcatatcg cacgcataga cggccagcat cggcccgacg tggatcagat
    33241 caaggtcatc atggcggatg tgtgcgggcg gttgtccgct cggctcggat acgacgccgg
    33301 ccaggcggcg ccgcgcgggg atctatagaa ttcccggcat ggacgccttt ttactcgtct
    33361 tcggcgctgg cctgctggcc ggcgccatga atgccctggc cgggggtggc tcgttcgtga
    33421 gcctgccggc gctgatcgcc gtcggcatcc cgtcggtgca ggccagcgcg tcaggcgcgg
    33481 tcgccctgtt ttcggggggc gccgccagtg cgtgggcgta tcgtgacggg ttggggccga
    33541 tcggcccggt gtccgtgcgc gccattttcc tggcgacagt ggtcggtggg gcgggcggtg
    33601 ccatcctgtt gctgctgact cccacgaaag tctttgatgt gttggtgcca tggctcatgc
    33661 ttctggcctt gctggcgctg gctttcggca aacggctggg cgagtggctg cgccagcgct
    33721 atcggatcgg cgcggcggcg gtgttggcca tacagttcgt tctgggggct tacggaggat
    33781 atttcggcgg cgcggtcggg ctcatgatga tggcggtgtg gggtttgctg gaaagccggg
    33841 acttgaaaag cctgagcgcg ccccgcacgc tgatggtggt ggccgcgcgc accatcgcca
    33901 tactcatttt catcggtgcg gatgccgtgc gctggccgga gaccctggtc atgctcgtcg
    33961 ccgcagtgat cggggggtat gttggggctt tggcggggcg ccgcgcgccg gcaggcctga
    34021 ttcggggcgg aacactgctg ctcacgacag gcatcacggt ggcgtttttc ctccgggcct
    34081 acggaggcta gtctccggct cggggccgtt catgtgccgc acggggagtt ccctggtgct
    34141 tcgtgttccg ccgctgtcgt ccctgcgctt tgaccccgcc taccgaaaga tctcgcgacg
    34201 ctattacgaa cgcccggacg aattcgccga tgcgttcgct cgtgcatggt tcaagctgac
    34261 ccatcgcgac atggggccga agacgcgcta ccttggtccg gaaattccga ggaagacccg
    34321 atctggcaag acccgctgcc cgcagccatg ctgccggcgc ccagtgcgtc ggacaccgcc
    34381 gccttgaaag cgagggtcgc ggccccgggc ctgaccgttt ctgaattggt ggtcaccgcg
    34441 tgggcctcga catccacctt tcgtggcggc gacaagcgtg gtgtattcgt gacccggccc
    34501 gctacgttga gcaatgactt cttcgtcaat ctgctggaca taggcaccga gtggaggcaa
    34561 gccgccgatg acgcctatga aggcaaggac cgcaagacgg gcaccagccg atggatcgga
    34621 acgcgggccg atctgatatt cggctccaac tccgaactgc gcgccgaggc cgaggtctat
    34681 gccagttccg gcgccaagga gaaattcgtg aaggacttca ttgccgcgct gcttgtatcc
    34741 gcggtctatg gggggcgtct gccttgttgc agccgctcat tggcgagcat tttcagtcca
    34801 tcgtaaggac aatgattctt atttgcaata gaataatcgt tccaaaagtt ctttgtccat
    34861 ctatctattt ttggacaatt cattcccgga gtctggattc atgcaaaagc tcgatcgccg
    34921 aacactgttg cgcaccggtg tcgtagtggg ttccaccgcc ttgctgggcg cacgcggcct
    34981 gcgcgccgcc tgggccgacg aagcgcggac gctcacgttg tacaacgggc agcacacgca
    35041 caccaccgcg gcgctggtcg acgctttcac caaatccacg ggcatcaagg ttgccatacg
    35101 caagggcagc agctcgcaac tcgccaatca gatcatcgag gaaggttcgc attctcctgc
    35161 cgacgtgttt tattccgagg agagcccgcc cgtggtgaag ctggcggaga aagggctgct
    35221 ggcgcagttg gatcccaaga ccctcaagca agtccgcccg ggttacacgt cccgcaaagg
    35281 tgactggatc ggggtcacgg cgcgttgccg cgtcatcgcc tataacacca acatggtcaa
    35341 ggagagcgaa ctgcccaggt cggtgctcga catggcgacc gccaagtgga agggcaaggt
    35401 cgccttcgtg ccgaccagcg gcgccttcca ggagcagatc gtcgcgatcg agcaattgaa
    35461 gggccgtgcg gtggcgttga aatggttgga agggttgaag aaatacggcg tggtctacaa
    35521 cgggaacatg gcggcgatga aggcggtgga gcgcggcgag atcgccaccg cgctcatcaa
    35581 caactattac tggttcgtgg tggcccagga agtgggagcc aagaacatgc attcggcgct
    35641 gtactacacc ggcgacaagg accctggcgc gttgatcacc gtctccgccg ccggcatact
    35701 caaaacttcg aaaaaacaag cgctggcgca gaagctgatg gcctatatgg tcagcccgga
    35761 gggacagacg gtcatcgccg cgtcggtggc ggagtatccg ttgcgtccgg gcatcaagtc
    35821 gccgtacgac gtcaagccgt tcgatgagct cgacccgccg cccatcacgc ccgccgatct
    35881 cggcgatgcg ggtgatgcgc tgattttgcg gcgcgaggcg gggttggcct gattctgtag
    35941 cgatcgattc cactatgaat ttcagcggct cgcagcggcg gggaggaccc tcgatctggt
    36001 tcgcggggtc ggccctggtg cccgccgcgc tgatttccct gccgctggtc tatgtggcat
    36061 tgcgcgcgac ccaggccggc gtggtcggca tccacaccga actgttccgt tcgcgcacct
    36121 tgatgctgtt gatcaacacg ctggtcatca cggtcagcgt cacgatcctg tccagcctga
    36181 tcggcctggc cgtggcctgg tgcgtcgagc gcaccgatct gccggggcgg cgtgtgtggc
    36241 gcattctggc gggtgtgccg ctggcggtgc ccgcgttcgt gtccagctac gcctggtcgt
    36301 ccatcagtcc gatgttccag ggtatggcgg gggcgattct ggtgctgtcg ctggccagct
    36361 acccgctggt gtacttgccg gtggccgccg ggttgcgcgg cgtcgatccg ggtttcgaag
    36421 acgtggcgcg gtcattgggt gatggcccct ggcgctgctt cctgcgcgcg gtgctgccgc
    36481 aactgcgccc ggcgctgggc ggcggcgcgt tgctggtgct cacccacatg ctggcggaat
    36541 tcggcgcgtt ttccatgctg cgcgtgcaga ccttcacgac tgcgattttc gaatcctatc
    36601 aactgcagtt cgatagttcc tcggcagcgc tgcagtccat tgtcctcatg gccttgtgcc
    36661 tgccggcggc ctacggtgag atgcggctgc gcgccggcac gcgcgtggcg agggtggggc
    36721 ggggcagcgc gcgcgcggca agcactgtgc gtcttggccg gctgcgccat ggtgcgctcg
    36781 cggggttcgt cgcgctggtc gtgctcgcgc tcggcgtgcc tctggcaaca ctggcttact
    36841 ggttggcggt gggccattcg gcgggccggg ggtatgccga gatcctgccg gccatcggcg
    36901 gcaccgtggc gctggcttcc acgggcgcgg tggtgaccag tctgttcgcc gtgccgttgg
    36961 tgctgctgtc attgcgccat cgcggctggc tcgcgcagct tgcagatcgt ctgccgtaca
    37021 tcatccacgg gctgcccggc gtggttgtgg ccttggccat ggtgttcatg tccattcacc
    37081 tcgtgccggc cctgtatcag ggcgttgcgg tcctgctgtt ggcctacgcc attttgtatt
    37141 tgcctctagc ccagtcatcg ttgcgcgcgt cggcggaatt ggtgtcccca caactggaaa
    37201 ttgttgcgcg tggcctgggg cgccgtccgc tcgcggcatt ccttaccgcc acgttgccga
    37261 acatcgcgcc aggcatcggc gcggcgctgg cgttgatggt gctcgagatc gcgcgggaac
    37321 tgaccgcgac cctgatgctg gcgcccacgg gggtcacgac cttggcgacg caggtgtggt
    37381 cgcataccta cgacgccgaa tacgcggcgg cggcgccgtt cgccgccttg ctggtgctga
    37441 tctccattgt gccggtctac ttcttcacgc agcggttcct gcaattcgga gaatcgaaat
    37501 gagtcgtatc catgtcgatc aggtcgacaa gtcctatgag gggaacaccg tgctgcatgg
    37561 tgtttcactg gacatcgacg atgggtcggt ggtcgcgctg ctcggaccct ctggctgcgg
    37621 gaagacgacg ttgttgcgtt tgttggcggg gttcgattgg gtggattccg gcactatccg
    37681 gctcgatgac cgggtggtgg cggcgcccac cctgcacgtg ccgcctgagc gtcgtggcgt
    37741 ggggtatgtc ccgcaagagg gggcgctgtt cccgcacctg gatgtgcagg gcaacgtgcg
    37801 ttatgggttg tcgggccgtg cggactggcg cgcgcgggtg ggcgaagtcc tggcgttgac
    37861 cggtctttcc ggattggagc ggcgctatcc ccatgaactg tctggtgggc agcagcaaag
    37921 ggtggcgctg gcgcgggcgc tggcaccccg gccggcgctg attctgctgg atgaaccctt
    37981 caacgcgctc gacctggacc tgcgacgcgg catgtgcgaa gacgtcatca cgttgctgcg
    38041 caagacgggc acgaccacgg tgctggtcac ccatgacccc gccgaggcgt tcgcggtctc
    38101 ggatcgcatc gccgtgatgc atgagggccg gatcatgcag tgtgccgagc ccgacgtggt
    38161 ctattggcgc ccggccagcg caccggtggc gcgcttgacc gggtccgtgg tgtttctgga
    38221 tggcacggtc aacgatgggc atgtggaaac cccagtgggg ccggtgccgt tgtatccgtc
    38281 acgcgccatg cctgcgggtt cggcctgtgc catgctgcgg ccggaacaga tcacgctggg
    38341 gccaccaggg cgcggcatac gcgcccgcgt cgcgaagcgc actttccatg gcgaccatgt
    38401 gctgctgggc gtggtggtgg acgggctgct ggagttgaac ctgcgtatga cgtcctgggc
    38461 cgcgccggcg tcagggtcgg agattttcct gtctgtgcat ggatcatgcg tggcgttcgc
    38521 cccagacgac gcatgactca cgcccgcaat cgatacccgg tccgaaagat ccacgccacg
    38581 atgcccaggc atatcgccag aaacactagc gtcatcgcca ggctgaccgg cacgctgacg
    38641 tcggccaggc cgtaaaagct ccagcggaac ccgctgatga ggtacaccac tggattgaac
    38701 aacgtgacct tctgccagaa cggtggcagc atgtcgatgg aatagaagct gccgcccagg
    38761 aaggtcagcg gtgtgacgat cagcaacggt acgagctgca gtttctcgaa attatcggcc
    38821 cagatgccga tgatgaagcc gaacaggctg aacgtgatgg cggtgagcag caggaacaga
    38881 atcatcaccg ctggatgatc gatgcgtacc ggcacgaaca ggcaagccgt ggccaggatg
    38941 atgacgccca ggatgacgga tttggtggcg gccgcgccca cgtatcccag cagcgcctcg
    39001 aagtacgaca cgggtgccga cagaatctcg tagatggtgc cggtgaactt cgggaaatag
    39061 atgccgaacg acgcgttgga gatgctttgc gtgagcaatg acagcatgat caggcccggc
    39121 acgatgaagg ccccgtagct gacaccgccg atctggttga tgcgtccgcc tatggcggcg
    39181 ccgaacacga cgaaatacag tgatgtggag atcaccggcg cgacgatgct ctgcatcagc
    39241 gtgcgccagg tccgggccat ttcgaattga tagattgcct tgacagcacc caggttcatg
    39301 catgttcctt gaccagatcg acgaagatgt cctcgagcga gctttgtgac gtttccagat
    39361 ccttgaaggc gaggccctgt gcgccgacag ccgccagcag ggcgggaatg ccggtgtgtt
    39421 cctgctgggc atcataggtg tagaccaggt cgccgccgtc agcgatggcc aatggccaag
    39481 aggtcaacgc gtcgggaatc tgtgaaaggg gtttctgcag ccgcaggatg agttgcttct
    39541 tgcccagctt gcgcatcagc gtggctttct cttccaccag cacgatttcg ccgtgatcga
    39601 tgacgccgat gcggtcggcc atttcttccg cttcctcgat atagtgcgtg gtcaggatga
    39661 ccgtgacgcc ggtggcgcgc agcgagcgga ccatgtccca catgcctttg cgcagcgaaa
    39721 cgtccacccc cgcggtcggt tcgtcgagga acagaatgga cggttcgtgt gacagggcct
    39781 tggcgatgag cacacggcgc ttcatgccac cggatagcgt cagcagcttg ttgtccttct
    39841 tttcccacag cgacaggtcg cgcagcacct tctctatgtg ggccggattg gtgggcttgc
    39901 cgaaaagccc gcggctgaag ctgaccgtgt tccagaccgt ttcgaacgcg tcggtcgtga
    39961 gttcctgcgg caccaggccg atgcgggcgc gtgccgcgcg ataatccgtg atgatgtcgt
    40021 ggccgtcggc caacaccgtg ccacgggtgg ggttgaccag gccgcagatg atgctgatga
    40081 gcgtggtctt gccggcccca ttgggcccca gcagggcgaa gatttcgcct tgccggatat
    40141 ccaggttgat gttcttgagc gctcggtggc ccgaggcgta ggttttatcg agattggaga
    40201 tggtgatgat cgcgggcatg acggctttgt gtggtctggg ggcgagtcgg cccgatgaat
    40261 atcgcaaaag agtatcacgg ccgtggcgaa gcgtgatgtt atccttgtcc tacccgccac
    40321 cgtcaaggat ggtggcgcac tcgttcatgt tcagggttcg cccgggcttg gcgacgcgct
    40381 cgcagtctgc gcggacctcc agccgcaagg aggcgatccc gtgtcgtttt gcacaattcc
    40441 cgataaattc cgatgccgca tgggtgcact gggcatggcg cggtggttcg cggcatgctt
    40501 gctggccgtg ctgctgggca ttgccatcca gccagcccgc gccgccgtgc catccgctca
    40561 gtcggcccgg tcatccgcgg cgccatcata cggcgcacta gccagtctgc tcgaaaacaa
    40621 ggattcccgc gatgccttga tcaagcagtt gcgtacgctg gccgcgaaat cggaaggcaa
    40681 gctcagtggc cagcaagcgc ctcccgtgcc aacgggcgaa accgctggcg ggcaggctgg
    40741 gttcctggct ggggcgggcg acatggcggc gacgttgcag tcgttcacgg tttcctttag
    40801 ccataatgtg gaaaccatgg ccgacatgtt cagccgtcgt ggctgggtcg ccggcgccta
    40861 cgtcaagggg ctggaaacgg tgcccggttt actggccgtc attgcgtcca caatcattgc
    40921 gttgttcgtg ctgagggcgt gcgcgatgtg ggcctatcgc cgtctcgatg actgggtgtc
    40981 ccgtttgggt gcggatctgg cgaccactgg ccatcgtgca ttggcgccgc gtgagcttta
    41041 ttgcaagatg ggcgccatcg cgctgggctt cctggtcgat cttgccacgg tgctgctggc
    41101 cgcggcggtg ggatatgggg tcgtgttgta tggggtggta cgccatcttt ccgcccaggc
    41161 gcctggggcg gtgttcgtga gcgctttcct tgccgtcgag gccacctgtg cgctggctcg
    41221 ggtggtgttc gccacacgct acccggattt gcgcctgatc cgcatgaccg atggcgtggc
    41281 cggtttctgg aatgcctggc ttgcgcggct gatccgcatc acggggtatg ggctgctggt
    41341 cgcggtgccc gtggcacaaa cgcttctgct gtccgcggcc ggcaaggtgc tgggcctgct
    41401 catcatgctg ggcgtctacg tctatgcggt gcgcgtgatc tggaccaacc gccatctgtt
    41461 gcgagagcgc atgatggccc gtgcggccga gctttccgca cccttgatga gcaccttgct
    41521 gcgggtgctg gcacgcatat ggcatgtgct gggaatcgcc tatttcacgg aattgctgat
    41581 cgccagccag gtcaatcccg aagatgcgct gcccttcatg gcgcaggcca cgatccagac
    41641 attggccgcg gtgggtgtgg gtgtgctgtt gtcactgatc ctgacggcga tgctgtcacg
    41701 tcgcatccat ctttctgaag acatgcgtgc ccgtctgccc atgctcgaga accgcatcaa
    41761 cgcctatgtg cccgctacgc tccgggccct gcgcctgttc attctgtttg tggtggcgct
    41821 ggtcgtgctc gatgcctggc acgcgttcaa tctggctgcg tggatggtct ctgcgtcggg
    41881 gcgggcggtg ctgacgacgg ttgtgcgcgt ggctgtcatc ctgctggccg ccgccttcgt
    41941 gtggacggtg gtggcgagca tcatcgagag ccggctcagc ggtggaacgg gtcgtcacag
    42001 gccgagcgac agggagaaga cgctgctttc tttgtttcgt aatgcgtcgc tgatcgtgat
    42061 catcactttt accgtgctgg tggtgttgtc acaggtaggc atagccatcg gcccgcttat
    42121 tgccggcgct ggcgtcgtcg ggctggccat cggttttggc gcgcagaaat tggtgcagga
    42181 cgtcatcacc ggcgtcttca tccagcttga gaacggtatg aaccagaatg atgtggtgga
    42241 gctggctggg ctgttcggca ccgtagagaa gatcaccatc cgttccgtgg tggtgcgcac
    42301 gctggatggt ggctatcact tgattccgtt ttccacggtg gacaagctca ccaaccatac
    42361 acgggatttc ggctaccact acggcgagta caacatcggt taccgcgagg atgcggacaa
    42421 cgtcatcgcc catctggagc atgcgttcga cgagctcatg caggattccg aactggctcc
    42481 tcacgtgatg gagcgcatct ccatcccggg tgttacgtcg ctgaccgaac gcggtttcac
    42541 ggttcgagtt ctcatcaaga cggtgccggg tatgcagtgg atgattcagc gcggcttcaa
    42601 ccggctggtg aagaagcatt tcgatgccgc cggcatagaa atgccgtatc cgcactcggt
    42661 ggtgcagttc gaaggcggac gggatggcgg catggtgccg ctgcgcgtac gcatggggca
    42721 atcgaggtcc gaagggccgc gcgccgaagg ctcgggatcc tgtgctccgc accccgcgcc
    42781 acagggtacg gcgtgacgtt caccgatctc gcgccggggt atcatgtttg ttcggccccc
    42841 gttggtggcc gtccggagga ttgaaccctt cattctcgag caaggaggcc accatgcctg
    42901 caatcggagg cgcattgctg acgtcggagc agatgcggcg tgtggaccag gcagccgtgg
    42961 cgtcgggcgt gcccggcatc gagttgatgg aggccgcagg tgcggccgtg gctcggcggg
    43021 ttgccgggcg ttggaacagg cggccggtgt tggtgctttg cggaccgggc aacaatgggg
    43081 gagacggttt cgtggccgcg cgccatcttg tcgatgctgg ttggcctgtg cgcgtcggcc
    43141 tcctgggttc tctggcacga ctcgcgggtg atgccgccca ccatgcggaa cagtggggcg
    43201 ggacaacagt cgacgccgac ccctcgctgt tcgaaggggc gggcttggtg atcgacgcac
    43261 tgttcggttc aggattgtcg cgtccgctgg acgggctggc tgccgacctg gtgcgggcgc
    43321 tcggagccag cgccatccct gtttgcgcgg tcgatgttcc cagcggccta tcggccgata
    43381 cgggcgaagt gctcggtgtg gccgtcgcgg ccgacgaaac cgtgaccttc tgccgcaaga
    43441 agcccggtca tctgctcgcg cccgggcggc aactgtgcgg cgtgctcacc gtggccgata
    43501 tcggcattcc cgaggcggcg cttgacacgg tgtctccccg tgtattccag aatggcccgg
    43561 ggcaatggct ggatcagtac ccctggtgcc ggcccgacac tcacaaatac cggcgtgggc
    43621 acgtattggt gctgggtggg gcggtgcaga ccggcgcggc gcgtctggtg gccttgggcg
    43681 cggcgcgggc gggcgccggc ctggtgactg tggccgcgcc ggattcggtc tggccgattt
    43741 acgcggcggc gctcaccagt gtcatggcgc agccggttca tggccccgac ggtttccggg
    43801 cgctgcttgc cgataaacgc aagaatgtgg tggttctggg gcccggcgcg ggggcgtccc
    43861 ccgagacgcg gcaatgtgtc ttgtcggctc tggagacggg ccgcggcgtc gtgctcgatg
    43921 ccgacgcgct caccgtgttt tccggcgaag tcgaagtact ggccgatgcc attgccggac
    43981 cgtgcgtgct gactccccac gaaggcgagt tcgagcgtct gttttcgttg cggggcgaca
    44041 agcttgcgcg cgcccgcttc gccgctcgtc aaagtggcgc ggtggtggtg ctgaaagggt
    44101 gggacacggt ggtggccgcg cctgacggcc gcgccgtggt caatgccaat gcccctcccg
    44161 aactggcgac gggtgggtcg ggcgacgtgc tcgccggcct gatcggcggt ctgatggccc
    44221 agggcatggc gccattccag gccgcgtccg ccgcagtctg gctgcatggg gaggccgcca
    44281 cacagatcgg cccgggcctg attgcggaag acctgcccgc ggcgctgccg cccgttcttg
    44341 gccgattgaa ggcgctgaac atcgggttac actgatcact gcttgtcctt gcagtgttgc
    44401 cggccggcac ccaccggcca gaggttgtcc atgtccgacg tcatagccct ggtattcgat
    44461 ttcgacgata cgctcgcccc tgacagcaca tcggggtact tggcgcgaat gggcgttgac
    44521 gtcacacggt tctggaccga agaagtcggc ccgatgctga gccaggatga ttgggatccc
    44581 gtgcccgcct atctgtatca gatgatccgc ctgtcgaaca gcggcaacta tggccgtttc
    44641 acccgcgaga gcctgatggc gtggggccgc gacctgccgt tgcatgatgg agtggatacc
    44701 ttgttcgagc gcttgcgcgc cacggtgcgg gcccagcatc cacaaatcca gttggagttc
    44761 tatctgatct ccagtggtat cggcgacgtg gtccggagca cgccgatcgc gcgcgagttc
    44821 accgatatct gggcgtccga gttcaaatat gacccagacg ggggcatcga gtttcccaga
    44881 cgcgtggtca gcttcaccga caagacacgc tatttgttcc atatccagaa gggcatcatc
    44941 ggcccgacct cgcgcaacaa gcctttcgag gtcaatcgca aggtgccgga agacaaactg
    45001 cgcgtcccgt tcgagcaaat gattttcgtc ggcgatggct acaccgacat cccttgtttt
    45061 tcgctgatcc gccgctcggg cggtgtcgcg ttcggcgtct gggatccgcg gcacaaggag
    45121 aagcgcagcc gcgcctgggg gttcatccag gaggggcggg tgtcgaatct gaaccaggcc
    45181 cggtatggcg acgacgccga actgtatcaa tggctggagg aagcagtcga cagcctggcc
    45241 agccgcatcg cgatcaactc gcgcatgtat cgtggttgag tccggtcccg cgcagtctcc
    45301 ggttctggcg gcgagccccg cgttttccgc gcaggacacc gagcacatgc tgcgcgcgct
    45361 tgaacaggcg cgtctggcaa gcctgcaggg cgaagtgccc gtgggcgccg tggtggccga
    45421 tgaaaacggt gcgtgcatcg ccgagggctt caacagcacg gtcggcgcgc acgacccgac
    45481 cggccacgcc gaaatcgtcg cgttgcgccg ggcggcgcaa cgaatgggta attaccgcct
    45541 gcccgggaaa acgttatacg tgacactcga accctgcgtg atgtgcatgg gcgccatgct
    45601 gcacgcccgt ctggcgcggg tggtgtacgg cgcggccgac cccaagaccg gcgcctgcgg
    45661 cggggtgctc agtgtgcacg cggtcgtcga gctgaatcac cagacacgcg tcgaaggggg
    45721 gttgctggcc gaaccctgcg gagacattct caggcgtttc tttcgggcgc ggcggggcgg
    45781 cggtatcggc gcccagcctt gaatccttgc cttgcccagg gccgatgtca atatatccaa
    45841 tggtatcgta cgcagtggcg tgatcgccgc gaccggcggt cttcctgact acaaaagaat
    45901 ttttccctca tgactcattc ccacgaccat tccgagccgc cgcgcggcat ctacttgatt
    45961 tccccatctg gccaggtgcg cgaccccgcc gatctggcgc gggcgtgcga gcggctgtcc
    46021 accctgggat tcaagactaa ggtggatcgg gcggcgctcg ccgtgcacga acggttcgcc
    46081 ggcaccgaca agcagcgcct ggccggcatc acacgcgcgt tgcggcagcc gcatcccatc
    46141 gtgatggcca cgcgcggcgg atatgggttg agccggctgc tgcctctcat cgattggccc
    46201 gcggtcgccg aaagcggcaa gactttcatc ggccagagcg atttcaccgc cttcaatctc
    46261 gccttgctcg cacgcaccgg cgtggaaagc ctggctgggc cgacggccat cgccgacttc
    46321 ggcggggcca ctgtggatga cctgaccgag gcgctgttca ccgaattgct cgatcatcaa
    46381 ctggagatcc tgagcttcga aacccccgat gccgatccgg tcgatgcccg cggcgttcta
    46441 tggggtggca atctggccat ggttgcggcg ctggtgggca cgccgtacat gcccaaagtg
    46501 cgcgggggca ttcttttcct ggaggatgtg gccgagcacc cattccgggt cgagcgtgcc
    46561 ttggcccact tgtggcatgc cggtattctc gaccggcaga aagcgatcgt gctggggcat
    46621 ttcaccgact atcgtcttgg cccccatgaa aatgggttcg acatgccctc ggtgctgcgc
    46681 tggctcaggg ccacagtgaa agtcccggtg gtgacggggc tgccctacgg gcatattccc
    46741 accaaggcca ccttgccggt gggcaggaaa gtgggcatcg ccacggaaaa gggcacggcc
    46801 catctggtgt tgcacgaaca cgcctgattg ggggcggcca aggcgcgccg cccaccccgc
    46861 acggaagggg gctcatgaat gggccgagcg acgcatttgg gttcgaatac gtgtggccag
    46921 ggcatttgct aaaatgctag gttgtcttta acgctccacg gcacggccag tctcagtgat
    46981 ttccacttcc aacctcacga ttcaattcgg acccaaaccc ttattcgaga acgtcagcgt
    47041 caagttcggc ggcggcaacc gctacggact gatcggcgcc aatggctcgg ggaaatccac
    47101 cctgatgaaa atcatcggtg gcgatctcga accctccagc ggcaatgtgg cgctggatcc
    47161 tggcgtgcgc ctgggcaagc tccgccaaga ccagttcgcg tttgaggact tgcgtgttct
    47221 cgacgtggtg atgatggggc atcacgagat gtggcaggcc atgattgacc gcgacgccat
    47281 ctatgccaac ccggacgcca ccgacgagga ctacatgcgc gcggccgacc tggaggcgcg
    47341 tttcgccgaa tacggcggct atacggctga agcccgcgcg ggcgaactgc tgctggggct
    47401 cgagctcacc atagagcagc acaaccagcc catgcgcgag atcgcgccgg gttggaagct
    47461 gcgtattctg ctggcccagg cgttgttctc gaaccccgac gtcctgttgc tcgacgagcc
    47521 caccaacaac ctggacatca acaccatccg ctggctcgag aacgtgctca atggctatca
    47581 gagcacgatg atcatcatca gccacgaccg gcatttcctg aacgaggtct gcacgcacat
    47641 ggccgacctg gattatggcg aagtccgtgt gtacccaggg aactacgacg actacatgct
    47701 ggcctcgacc caggcgcgcg agcgcgtcac ggccgccaat gccaaggcca aggaacgcgt
    47761 ggccgatctg caggatttcg tgcgccgctt cgcggccaac aagtccaagt cgcgccaagc
    47821 cacgtcgcgc ctgaagcaga tcgaccgcat caaggccgat accgtcgagg tcaagccgtc
    47881 gtcgcgtcag aatccctata tccgcttcga gcaagagaaa gcgctgcacc gcctggcgat
    47941 caccacggaa tccatttcca aggcgttcga caagcccgtg atccggccat tctccaccat
    48001 gatagaggcc gggcagaaga tcgccatcat tggcgccaat ggcgttggca agacgacctt
    48061 gctgcgcctg ctggcagccg acctggaacc cgattcgggg tcgttgaaat acgccgagaa
    48121 cgccgacctg ggttacatgg cccaggacgt ttccggccag ttccagtcgg atagcaacct
    48181 gtttgactgg atgggcgatt accgcaagcc gggcgatgac gatcaggcca tgcgatctgt
    48241 gctgggccgt ttgctgtttt cttcggacga cattcccaag aaagtccgtg tgctgtcggg
    48301 cggcgagaag aaccgcatga gctttggccg cctgatgctg gggcgccaca atgtgatgct
    48361 gctggacgag cccaccaacc atctggacat ggagtccatc gagtcgctgc agtacgcgtt
    48421 ggaaaaatac gcgggaacgc tggtgttcgt gtcgcacgac cggcagttcg tctccggcat
    48481 cgctgatcgc gtgatcgaga tcatgcccga tggcgacatt gtcgattatc gcgggaacta
    48541 cgacgactac ttggcctcgc gcgggttgta acgcgccggg gattcaggtc attggacggg
    48601 gctggtgttt aaggtgctgt ccgccggggc cagggggtgg cgcaggcgga cagctcacgg
    48661 tgatggcatg cggggaggtt gatcggggcc gctgggcaat ttgccaacgg cgcctgttcc
    48721 ctaggtggac gcattggctc cgttcgcctg ctcttgctgg gcgatttctt cgtgcacctt
    48781 ggccatgtcc accgccttga cctgggtgat caattcgttg agttgcgttg gcgacagcgc
    48841 acccgcttgc gagaacaaca acactttctc gcggaagacc atgagggtgg ggatggagcg
    48901 gatgcccatg cccgcggcga tttcctgctg ttcttcggtg ttcaccttgg cgaagacgat
    48961 gtcatcgtgc tggccggacg ccgcctcgaa ggtgggggcg aagctgcggc acggcccg
//
LOCUS       DASTNK010000036         6449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_765123, whole genome shotgun sequence.
ACCESSION   DASTNK010000036 DASTNK010000000
VERSION     DASTNK010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765123"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1980)
                     /gene="cyoB"
                     /locus_tag="VFR20_03055"
     CDS             complement(<1..1980)
                     /gene="cyoB"
                     /locus_tag="VFR20_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HEU0229526.1"
                     /translation="MPDRSPILNFLLGRLTLDSFPFLSPDLEGRIVLVTLIVVAVVGL
                     CILGLVTKKRLWGYLWHEWFTSVDHKKLGIMYIILATVMLLRGFSDAIMMRAQQAVAF
                     GPHMGYLPPEHYDQIFSAHGSIMIFFVAMAYIVGLMNFVMPLQIGSRDVAFPFLNNLS
                     FWLLVSGVILFMTSLFVGDFSTAGWLGYPPVSELHQSPGPGVDYYIWGLQLSGIGTLL
                     TGINFVVTVIKMRAPGMTMMKMPIFSWTTLCTSGLIIVTFPILTATLFMLSLDRYLDM
                     HFFTNGFGGNVMLYINLIWVWGHPEVYILILPIFGVYSEIVATFSRKPLFGYSSMVYA
                     TMAIMILSYLVWLHHFFTMGSGADVNSFFSLTTMIISIPTGAKIFNWLFTMYRGRVQY
                     TVPMLWTMGFLFTFTIGGMTGVMLSVAPADFLLHNSLFLIAHFHNVIIGGVVFGIFAA
                     INYWFPKAFGFRLNEFWGKLSFWFWQIGFWVAFGPLYVLGLMGVTRRLSHFDDPSLQI
                     WFVVAAFGAFLILLGILAFIIQIYVSIVRRKEYAVGNDPWDGRTLEWSTSSPPPDYNF
                     AFIPQVHERDAWTDMKEQGVRRPLEGFNDIHMPSNTGTGVYIGILGTAFGFAMVWYIW
                     WLAVVSFLGIIVVSIAHTFNYHRDYHVPAEEVNQFE"
     gene            complement(2004..2963)
                     /gene="cyoA"
                     /locus_tag="VFR20_03060"
     CDS             complement(2004..2963)
                     /gene="cyoA"
                     /locus_tag="VFR20_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HEU0229527.1"
                     /translation="MLVKRFCRLLLLPVMLVLGGCNLVLLNPDGYVARQQSDIMISTT
                     IIIALIIVPVLIAIAIIAWRYRASNTKATYDSKWDHSPQLELLVWAVPLLIIIAVGAV
                     SWTGTHQLDPYKPLDKIADNKPVPTNAKPLEVEVVSLQWKWLFFYPQYGIATVNELAA
                     PVNVPIHFKLTADTMMDSFFVPNLAGQIYTMPGMQTVLHGVINKPGDYKGFSANYSGA
                     GFTDMRFTFHGLSQKAFNNWVDKVRAKGQTLDRTTYDKLKQPTRAEPIHYYAHFSPDL
                     YERILNRCVDPGQMCMNGMMTADAMPSRGMDTHTPAHDSAAMH"
     gene            3126..4463
                     /locus_tag="VFR20_03065"
     CDS             3126..4463
                     /locus_tag="VFR20_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0229528.1"
                     /translation="MSSTSHAQPVGPSGLAPSQSGSHDRPITPGDISVGVVVGRTAGF
                     FDVFVFGIACALVFPGVFFPFANSADGMFYSFAVFALGFVAQPLGSLFFLPLERRFGR
                     ATALTAALFLLGCSTVGVAFLPSYSSIGYASIVALCVLRVGQGFAMGGSWDGLPSLLA
                     MKAPKDSRGWYASIPQLGAPIGFIVAAALFAYLWSTLSYEEFFGWGWRYPFFAAFAVN
                     VVALFARLRLVVSPEYRQEFRQRELAPSKVGGLAGSGQGKYVLLGAFAPMASYALLNL
                     VTLFTLSWALLYTQQSPTTFLLIQIIGAIVAIPCMLISGRIADRLGRRVTLGLFATLI
                     AVYSGWTAVLLSGGDYESYVFIILGFALLGFSHALSSGAVAASFRSEFRYTGARFSAD
                     LSWLLGAGFAPLVALVLADYFGVGYVGLYLLSGAFCTLAALWVSRRLGGLPNN"
     gene            complement(4632..5273)
                     /locus_tag="VFR20_03070"
     CDS             complement(4632..5273)
                     /locus_tag="VFR20_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEU0229529.1"
                     /translation="MADFDVLETLALRGGAFVHLPLHLDRMQATAQHFGYPWIAPRIT
                     QVLALLRDQHPQGDWRVRLALRHDGKVMGEAHPLRPTPTPVRLQLARAPLANALAHGD
                     WVHFKTSRRDHYVALQPADASVFDTVLFNEDRELTETTRGNIAVLLDGRWITPPLRCG
                     LLPGVGRQVCIEQGRLAERVILLDDVPRAQGWAFVNSLRGWLDAERVEAALDK"
     gene            complement(5308..>6449)
                     /gene="pabB"
                     /locus_tag="VFR20_03075"
     CDS             complement(5308..>6449)
                     /gene="pabB"
                     /locus_tag="VFR20_03075"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEU0229530.1"
                     /translation="MNSRIDFPDPRDATAPRLGRCFGAPKAVLSAHRPEDVASLLNAV
                     EHAAQTGHWCVGWLRYEAALAFDAAFADALHPVPHGEPLVWFAVHDRALDWSTEGTAD
                     NEASQAAWQPGIDRAAFDLAITAIHRAIAAGDCYQVNLTSQLEGRLQGNASSLFTALR
                     RAQPMGYVAAIDNGTEQILSVSPELFFDWDGERILTRPMKGTAPRGATPAEDSAQVEA
                     MRASSKERAENVMIVDLLRNDLSRIALPNSVRVPRLFQAEALPTVWQMTSDVEAVTRP
                     GTRLADVFAALFPCGSITGAPKRQAMRWIRRLEPGPRGVYCGALGVVRPASTPGGMHA
                     TFNVPIRTVTVRGNVLRCGIGSGITASSSADAEWQEWRHKRLFLG"
BASE COUNT         1256 a         1848 c         2018 g         1327 t
ORIGIN      
        1 ctcgaactgg ttcacctctt cggccgggac gtgatagtca cggtgataat tgaaagtgtg
       61 ggcgatggac acgacgatga tgcccaggaa cgacacgacg gccagccacc atatgtacca
      121 gaccatggcg aagccgaagg ccgtgcccaa tatgccgatg tagacgcctg tgccggtgtt
      181 gctcggcata tggatgtcgt tgaacccttc cagtgggcga cgcacgcctt gctccttcat
      241 gtcggtccag gcgtcacgct cgtgcacctg cgggatgaac gcgaagttgt agtcgggcgg
      301 cggcgaggag gtcgaccatt ccagtgtgcg gccgtcccag gggtcgttgc ccaccgcata
      361 ttccttgcgg cggacgatac tgacgtagat ctggatgatg aacgccagaa tgcccaacaa
      421 gatcaggaag gcgccaaagg cggcgacgac aaaccagatc tgcaggctgg ggtcgtcgaa
      481 atggctcagg cgacgggtca cacccatcag gcccagcaca tacaacggac caaaagccac
      541 ccagaaaccg atctgccaga accagaacga cagcttgccc cagaactcat tcaggcggaa
      601 gccgaaggcc ttggggaacc agtagttgat ggcggcgaag atgccgaaca ccacacctcc
      661 gatgatgaca ttgtggaagt gcgcgatcag gaacaggctg ttgtgcagca ggaagtccgc
      721 tggtgccact gacagcatca cgccggtcat gccgccgatg gtgaacgtga atagaaagcc
      781 catcgtccag agcatcggta cggtgtattg aacccggccg cggtacatgg tgaatagcca
      841 gttgaagatc ttcgctccgg ttgggatcga gatgatcatg gtcgtcaacg agaaaaacga
      901 attgacgtcg gcgcccgagc ccatcgtgaa aaagtggtgc agccacacca ggtacgacag
      961 gatcatgatc gccatcgtgg cgtagaccat cgacgaataa ccgaacaacg gtttgcgcga
     1021 gaacgtcgcg acgatctcag agtacacgcc gaaaatgggc aggatcagga tatacacctc
     1081 ggggtgaccc catacccaga taaggttgat gtacagcatg acgttgccgc caaaaccgtt
     1141 ggtgaagaaa tgcatgtcca gatagcggtc cagcgacagc atgaacaacg tggcggtcag
     1201 gatcgggaag gtgacgatga tcaggccgct ggtgcacagc gtggtccagc tgaagatcgg
     1261 cattttcatc atcgtcatgc ccggcgcgcg catcttgatg acggtgacga cgaaattgat
     1321 gccggtcaac agcgttccga tgcctgatag ctgcaagccc cagatgtagt agtccacgcc
     1381 tggtccgggg ctttgatgaa gctcggacac cggggggtag cccagccagc cagcggtgga
     1441 gaaatccccc acgaacaacg aagtcatgaa cagtatgacc cccgaaacca gcagccagaa
     1501 gctcaggttg ttcagaaacg ggaaagcgac atcgcgcgac ccgatctgca gcggcatcac
     1561 gaaattcatc aggccgacga tatacgccat ggccacgaag aagatcatga tcgagccatg
     1621 cgccgagaag atctggtcgt agtgttcagg cggcagatag cccatgtgcg gaccgaaggc
     1681 gaccgcttgt tgcgcacgca tcatgatggc gtcggaaaag ccgcgcaaca gcatcacggt
     1741 ggcgaggatg atgtacatga tccccagctt cttgtggtcc acactggtga accattcgtg
     1801 ccacaggtat ccccatagcc gctttttggt gacgaggccg aggatgcaca ggccgacgac
     1861 cgccaccacg atgagggtga ctaggacgat gcggccttcg agatcggggc tcaggaatgg
     1921 gaaggagtcg agcgtcaggc gcccgagaag aaaattcagg atgggagaac gatcaggcat
     1981 agtaagcatt ccaggaaatc cgatcagtgc attgctgccg agtcatgcgc gggggtgtgg
     2041 gtgtccattc cgcggctggg catggcgtct gcggtcatca tgccgttcat gcacatctga
     2101 ccagggtcga cgcagcggtt gaggatgcgt tcgtacagat cgggactgaa atgggcgtag
     2161 tagtggatgg gttcggcgcg ggtgggttgc ttgagcttgt cgtaggtggt gcgatcgagg
     2221 gtttgaccct tggcgcgcac cttgtccacc cagttgttga aggctttctg gctcaggcca
     2281 tgaaaggtga agcgcatgtc ggtgaaaccg gctccgctgt agttggcgga aaaacccttg
     2341 tagtcgccgg gcttattgat cacgccgtgc agcacggttt gcatgccagg catggtgtag
     2401 atctgtcccg ccaggttcgg cacgaaaaag gaatccatca tcgtgtcagc cgtgagcttg
     2461 aagtgaatcg gcacgttgac cggtgcggcg agctcattca cggttgcgat accgtactgc
     2521 ggatagaaga acagccactt ccactgtagc gaaactactt ccacttccag tggtttggca
     2581 ttcgtgggca cgggcttgtt gtcagcgatc ttgtccagtg gtttgtaagg gtcgagctga
     2641 tgggtgccgg tccaactgac cgcgcccacc gcgatgatga tcagcaacgg caccgcccac
     2701 accagcagtt cgagctgtgg ggagtggtcc catttactgt cgtacgtggc ttttgtgttt
     2761 gatgcgcggt atcgccacgc gatgatggcg atggcgatga gcacggggac gatgatcagc
     2821 gcgatgatga tcgtcgtgct gatcatgatg tcgctttgtt gccgtgctac gtaaccgtcg
     2881 gggttgagca gaaccagatt gcagccccca agaacaagca ttacggggag cagcagcaac
     2941 cgacagaaac gtttcaccag catgagagaa cctaagagcg agaactaaag agaagagcga
     3001 gaactaaaga ggagaactta aagatgaatg gcaaatctta tttattttat aataatccgc
     3061 cgaaccaggg tcgagaaatt tctcgattgg accatttgta cggcggtttg ttcatcggaa
     3121 acactatgtc ctctactagt cacgctcagc ctgtggggcc atccggcctc gctccgtcac
     3181 agtccggctc ccacgaccgc cccatcactc ccggcgacat ttccgtcggc gtggtcgtcg
     3241 gacgcaccgc cggcttcttc gatgtgttcg tgttcggcat cgcctgcgcc ttggtgtttc
     3301 ccggtgtctt ttttccattc gccaattcgg cggatggcat gttctattcc tttgcggtgt
     3361 tcgcgctcgg ctttgtcgcg cagccgctgg gttcgctatt cttcctgccc ctagagcggc
     3421 gtttcggtcg cgccacggcc ttgaccgcgg cgttgttcct gttggggtgt tccacggtgg
     3481 gggtggcgtt cctgccgagc tattcatcca ttggctatgc gtccatcgtg gcgctgtgcg
     3541 ttctgcgcgt gggccaaggc ttcgcgatgg gtggcagttg ggacgggctg ccatcgctgc
     3601 tggccatgaa ggccccgaag gacagtcgtg gctggtatgc gtccattcct caacttggcg
     3661 cacccatcgg ttttatcgtt gccgccgcgc ttttcgccta tctgtggtcc acgctgtcgt
     3721 acgaagagtt cttcggctgg gggtggcgtt atccgttctt cgccgctttc gccgtgaacg
     3781 tggtggcgtt gtttgcgcgc ttgcgcctgg tcgttagccc ggaataccgg caagagttca
     3841 ggcagcgtga acttgcccca tcgaaagtcg gcgggttggc tggttccggt caaggcaagt
     3901 acgtgttgct gggggcgttt gcgccaatgg ccagttatgc gctgttgaac ctggtcacac
     3961 tgttcacact gtcgtgggcc ttgttgtaca cgcagcagtc ccccaccacg tttctgttga
     4021 ttcagatcat aggcgccatc gtggcgatcc cctgcatgct gatctccggc cgcatcgccg
     4081 accgactcgg tcgacgcgtg acgctgggcc tgttcgccac actcatcgcc gtatacagcg
     4141 ggtggaccgc ggtcctgctg tcgggggggg attacgaaag ctatgtcttc atcattctcg
     4201 ggttcgcctt gcttgggttc tcacatgcgc tgtcctccgg cgcggtggcg gccagcttcc
     4261 gaagcgaatt ccgctacacc ggcgcacgct tctccgcaga cctgagctgg ttgctgggcg
     4321 ccgggttcgc accattggtg gctctggttc tagctgatta tttcggggtc ggttacgtgg
     4381 gtctgtacct gctctcgggg gcgttctgca cgttggccgc cttgtgggtc agccgcagac
     4441 ttggtggtct gcccaacaac taagaattcc aggcagttac gaaagtccgg ccgaacctcg
     4501 atgattcggc cggatttttc attgattgcg gcgagaagcg ggcgccacgt cgcgtttcta
     4561 caggctgtcc ttcttcgcgt ttctacaggc tgtccttctt cgcgtttcga caggctgttc
     4621 ttcttcattt tctacttgtc gagggccgct tccacccgtt ccgcgtccag ccagccgcgc
     4681 agactgttca cgaatgccca accctgcgcg cgagggacgt catccagcag tatcacccgc
     4741 tcggctaggc gcccctgctc tatgcaaacc tgtcggccca caccaggcag caggccgcaa
     4801 cgcaacggcg gtgtgatcca gcggccatcg agcagtaccg cgatgttgcc gcgtgtcgtt
     4861 tcagtcaatt cacggtcttc attgaacaac accgtgtcga ataccgaggc gtcagcgggt
     4921 tgtagggcga catagtggtc gcgtcgcgaa gtcttgaagt gcacccagtc gccatgggcc
     4981 agggcgttgg ccaatggcgc ccgcgccagt tgcaggcgca cgggcgtggg tgtgggccgc
     5041 agcggatgcg cttcccccat cactttgcca tcgtgtcgca acgccaggcg cactcgccaa
     5101 tcgccttgtg gatgctgatc gcgcagcagc gccaacactt gagtgatgcg gggcgcgatc
     5161 catgggtaac cgaagtgttg ggcggtcgct tgcatgcggt ccaggtgcaa cggcaggtgt
     5221 acgaaggcgc cgccgcgtag cgccagggtt tcaagaacgt caaagtccgc cattcgatgt
     5281 gctcccgaat agggttgcca tgtggcgtca acccagaaac aggcgcttgt ggcgccactc
     5341 ttgccattcc gcgtcggcgc tgctactggc cgtgatgccg ctgccaatgc cacaacgcag
     5401 tacattcccg cgcaccgtca cggtgcggat tggaacgttg aacgtggcgt gcatgccacc
     5461 tggtgtcgag gctggacgca ccacgcccaa ggcgccacag tagacaccgc gcgggccggg
     5521 ctccaacctg cggatccagc gcatggcctg gcgcttgggc gccccggtga tcgaaccgca
     5581 tggaaacagc gcggcgaaca catccgccag ccgtgtgccg ggccgggtga cggcttccac
     5641 gtcggatgtc atctgccaca ctgtgggcag cgcttccgcc tgaaacaggc gtggcacccg
     5701 gacactgttc ggcagcgcga tgcgcgagag gtcgtttcgc agaagatcca cgatcatcac
     5761 gttctcggcc cgttccttgg atgatgcgcg catggcctcg acctgtgccg agtcttcggc
     5821 aggtgtcgcg ccgcgcgggg ccgtgccttt catggggcgg gtcaggatgc gttcgccgtc
     5881 ccagtcgaag aacagttcgg gcgataccga caggatctgc tcggtgccgt tgtcaatggc
     5941 ggccacataa cccatgggtt gcgcgcggcg caaggcggtg aatagtgagg acgcgttgcc
     6001 ctgcaagcgc ccctctaatt gagaggtgag gttgacctgg tagcagtcgc ccgccgcgat
     6061 cgcccggtgg atggcggtta ttgccaggtc gaaagcggcg cggtcgatgc cgggttgcca
     6121 ggctgcctgc gaagcttcat tgtcggcggt tccctctgtc gaccaatcca gcgctcgatc
     6181 gtgcacggcg aaccaaacca gcggctcccc gtgtggcaca gggtgcaacg cgtcggcgaa
     6241 ggccgcatcg aaagccagtg cggcttcgta acgcagccag cccacgcacc agtgtccggt
     6301 ttgcgcagcg tgctccactg cgttcagcag cgatgccacg tcttcaggac gatgcgcgga
     6361 cagcactgcc ttcggtgcgc cgaaacaccg acccagccgc ggtgcggtgg cgtcgcgcgg
     6421 atcaggaaag tcaatgcgag agttcatgt
//
LOCUS       DASTNK010000037        46728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1136393, whole genome shotgun sequence.
ACCESSION   DASTNK010000037 DASTNK010000000
VERSION     DASTNK010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 46728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46728
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136393"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFR20_03080"
     CDS             <1..600
                     /locus_tag="VFR20_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEU0229531.1"
                     /translation="LENDTGDVARRLGDEATFQIMAERLINTLNGLSFNRIVTADPHV
                     LHCLRNEYPAFGGHYPVIHHSALLDDLIRAQAIAPRALELDHTLTYHDPCYLARYNGE
                     TEAPRRVLEALGIKVREMTRNGLNSRCCGGGGGAPLTDIPGKRRIPDMRMDDARDIGA
                     QTVAVACPGCTAMLEGVVGPRPDVLDIAELVELSLGEAA"
     gene            707..1825
                     /locus_tag="VFR20_03085"
     CDS             707..1825
                     /locus_tag="VFR20_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEU0229532.1"
                     /translation="MNTRRRVDPRRPFIVTSLGRRRYAIGQGAISGNNITPDTPQTPH
                     APHDTPLRIHAPTDHHIMVVAHSERGVLPSAAQQAVAAAALLADARCAVLLVVFGPLA
                     ESAAACGADQVIVLDGENRFEPDLALRELDRLRQHYHPARIVMADDGVPTGDLGRRLA
                     AQHGLSIATHVVELTSDTATVYQRHRSAYARRALPDVVLLDPGCVDMRLPFQGRGEIV
                     DWAPEDGVASHYTDHGVSAVPASHLALEEADLIVSAGNGVRDLDGFHALADALGAAVG
                     ASRVAVDDGRFTRAQQVGATGTTVTSSVYIALGISGAVQHLQGIKDCRHVIAVNTDAS
                     APMIKRADLSIIDDAQAIMRALTTEVHAARNAADGATP"
     gene            1822..2607
                     /locus_tag="VFR20_03090"
     CDS             1822..2607
                     /locus_tag="VFR20_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011354185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEU0229533.1"
                     /translation="MTDDEHPLRIVVLVSEGRHPVSGVSRWSRNDARALSLAYSVASG
                     KPTVQVLHAGHDSPATRDYLALGVNHLDIVPVAEGVDVVDALEAHVHNADLILCGLCA
                     EGQEDSGMVPYLLAQRLGLPLLPSALDLAIDAGGITIGQFLPKGRRRRVTTTLPCVVT
                     VHPMAPLTLKYAYAGTRTGTITTLPHTPIHATGQRGMDWTVSPINARPIKLAAHERRS
                     GHARMLSATTTESRAGVVVDQGSAQEKATAVITYLRKHGLIDY"
     gene            2615..3898
                     /locus_tag="VFR20_03095"
     CDS             2615..3898
                     /locus_tag="VFR20_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEU0229534.1"
                     /translation="MNVIRMAVETDKLKISPDTAALIARRPPGRPLEAPFYTSRKIFE
                     IDMKLIFGQHWIYVATEAEIPEPGDYVTIDLLKNSIVLVRDDNGDVKAFHNVCRHRGS
                     RLCNEHTGSVGNLVCPYHQWTYDLEGKLLFAEHMGEDFDRDAHGLRPVHLRNLAGLLF
                     ICLADTPPDDFEAMRAAMEPYIRPHQLHMTKVAAQVDIVEECNWKLTIENNRECYHCA
                     ANHPELTASIFEYGFGYQASPSNQAQLTAYDKLVATRTADWEGTCSLPSEEIEHLDDR
                     TTGFRTQRLPLDQGGESETLNAKVACRKLLGDFQRADLGALSFWTQPNSWHHFMSDHI
                     VTFQVFPLSENRTLVRSTWLVHKDAVENTDYTLDNLTAVWNATNAQDRHLVEISQRGI
                     ESDAYEPGMYSPYAEVYVEKFCRWYIDRLTALTTA"
     gene            3923..5047
                     /locus_tag="VFR20_03100"
     CDS             3923..5047
                     /locus_tag="VFR20_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004548824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid-cluster NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0229535.1"
                     /translation="MDVSANRINQAGFWERIPPQWNGEEEEALICCTIIDETHDVKTF
                     TFRTPKGSLFHFAPGQFLTLELEIDGVQVNRCYTIASAPTRPHTVSITVKRTPGGVVS
                     NWLHDHLKVGDRVRVLPPGGTFTCADFPAKKYLFLSAGSGITPLMSMTRTHFDLADDR
                     DIVFLHSARTPDDIIFEQELRRLAYTQQNFRTYFVCEVKGTRRDWNGVNGYLSLALLQ
                     LSTPDFLEREVFICGPEPYMRSVKGMLGDAGFPMDHFHQESFSFETLQDTRAATEHEE
                     QAAPPVDATDIQMFTVKFQKSQREITCVSTQSVLDAARQAGVRLASSCTQGMCGTCKV
                     KLISGQVHMEHKGGIRQREIDQGYALLCCSRPLSDLVIDK"
     gene            complement(5132..5986)
                     /locus_tag="VFR20_03105"
     CDS             complement(5132..5986)
                     /locus_tag="VFR20_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU0229536.1"
                     /translation="MKLSSLFTALGLAATLGIASVAQAADKPEIKIGYVNGWADSVAT
                     SETAAAIIREKLHYPVKLVAVSAGIMWSGVARGDLDTSLSAWLPVTHAQYYKKFKKDV
                     VLEGIVEKDAKIGLVVPDYVKAKTVADLKKYKANYNGVITGIDAGAGVMDKAQKAIKA
                     YDLGFTLAASSGAGMAVALDRAIRAKKPIVVTGWQPHWMFGRYKLRFLSDPKKVFGES
                     ENVYAMINPKLEKKAPEVVAFLKKFQWKPGQIAKVMVDIKDGTKPSDAAKKWVAANEA
                     EVNGWVGK"
     gene            complement(6055..7068)
                     /locus_tag="VFR20_03110"
     CDS             complement(6055..7068)
                     /locus_tag="VFR20_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEU0229537.1"
                     /translation="MSRLAISDCTHFGFVLLPSFTLLSFSSAVEVLRMANHLSGTQTY
                     RWSMLHPDRDHVVASNGISVTARPLDPLDIPDIVFVCGGIDVKAACDPQTLSMLRMLA
                     ARGVLLGSLCTGAYALARAGLLNGYRCSTHWENLTALRQEFPSVGFEEEVFVVDRNRV
                     TCTGGAAPLDMMLHLISPKVGRDIRSSIASQFIVAHIRDARDRQRVPLSARVGTVRET
                     LMEVATLMEANIEEPLSLGELAQLSHSSQRQIQRMFRDHLGVTPTQYYLNLRLQRARE
                     LLTQTGLSVMNITVACGFQSACHFSKTYRKLFGVTPSSERGWRFPHTKSLYQNSSSSC
                     LAQ"
     gene            complement(7215..7808)
                     /locus_tag="VFR20_03115"
     CDS             complement(7215..7808)
                     /locus_tag="VFR20_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HEU0229538.1"
                     /translation="MQTELRQESPLVGAGDLLDGGSATLSEQIRLCELPFLELNNIRG
                     NASDPDFRRAISEVIGVDLPVRANTVSEGPGYVLWWLGPDEWLAQSHDPMPARLDHDL
                     RVRFGDLFATTVDVSSGYTTLRLSGSRVSDVLNKGCPLDLHPRVFKPGQCAQSHFFKT
                     AIALRKLVDGTWHLIVRRSFADYATRMLVDASAEYGP"
     gene            complement(7798..10926)
                     /locus_tag="VFR20_03120"
     CDS             complement(7798..10926)
                     /locus_tag="VFR20_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit alpha family protein"
                     /protein_id="HEU0229539.1"
                     /translation="MSASGENLAQRRDGRSMADSTRSSDERAPSRDGGAAQGRTRHQP
                     GGSRRLESTGRVDRSQTVEFSFNGKTYHGFQGDTLASALLAQGVHFVARSWKYHRPRG
                     ILTAGVEEPNAIVQLEEGAYTIPNPRATEVELYSGLVASSVNASPSIEGDRLAFLQKL
                     ARFIPAGFYYKTFMWPRKLWPRYEERIREAAGLGTAPHLPDRDRYEKSFCHCDVLVVG
                     AGPAGLAAAHLAMRSGARVMLVEDRPRAGGALLREEAEIDGAPASAWVAERVAELEAS
                     PDVTFLTRATAFGHHDHNLVTVCHRLTDHLPVHSRRGVRERLLKVRARAVVLATGAHE
                     RPMVFGNNDLPGVMLASAISTYINEYGVLPGERAVVFTNNDDAYRTALDLARHGAQVK
                     VVDARPASGGTLPTLARGKGVTVMNEAIVIEARGTRHVSGVRIASYKNGVPGDIIADH
                     ACDLLGMSGGWNPVLHLFAQSGGKARWDDDKACFVPGNPAQPTFSAGAANGDFLLEQA
                     LEGGLAMARAALGRAGIEAVPTPAWRTPALREDPSVPVWFAGHKDQVGRAAKQFVDYQ
                     NDVAVSDIHLAVREGYESIEHVKRYTALGFGTDQGKLGNINGMAIVADLLGQTIPETG
                     TTTFRPNYTPVTFGVVAGRELGDFLDPIRKTCLHEWHARHRARFEDVGNWKRPWFYPM
                     EGEDLHAAVARECLAVRRSVGIMDSSTLGKIDIQGPDTVEFLNRIYTNSWTKLGVGKC
                     RYGLMLDENGMVFDDGVNIRLGQDHFLLSTTSGGAARVMAWMERWHQTEWPHLKVHMT
                     SVTDHFATFAVVGPHSRKVVGKVCAGIDFSDEAFPFMSFRDGAVAGVFARVMRISFSG
                     ERSYEVNVPANHGQAVWDLLMEAGREFGITPYGTEAMHVLRAEKGYIIVGQDTDGSVS
                     PFDLGMGGLVAKTKDFIGKRSLTRAHTAGPDRKQFVGLLADDPALVLPEGAQIIARQS
                     NERPVPMMGHVTSSYRSPTLGRTIALALLRDGLRRQGEKVTVSLSEGVSASATVCSPV
                     FYDPEGTRQNAD"
     gene            complement(10923..11204)
                     /locus_tag="VFR20_03125"
     CDS             complement(10923..11204)
                     /locus_tag="VFR20_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEU0229540.1"
                     /translation="MLLIECPWCGKRAETEFHCGGEADIARPVDGENLSDREWGDYVF
                     MRTNTLGVHHEQWVHAQGCRRWFKVERNTVTYEFGNYEKFPMPNSGEQA"
     gene            complement(11249..12493)
                     /locus_tag="VFR20_03130"
     CDS             complement(11249..12493)
                     /locus_tag="VFR20_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HEU0229541.1"
                     /translation="MNRYSIFSLVRNGLSYNRNWQRAWRSPEPRREYDVVIVGGGGHG
                     LATAYYLAKEHGVRNVAVLEKGWLGGGNTARNTTIVRSNYLWDESAQLYETAMKLWEG
                     LSQDLNYNVMFSQRGVMNLAHTLQEVRDTQRRVNANRLNGVDAEFITPAQIKEIVPII
                     NLNSRYPVMGASFQRRGGVARHDAVAWGFARGADKYGVDIIQNCPVTGIRRENGAVTG
                     VDTGKGFIKAAKVAVVTAGHSSVLASMAGIRLPLESHPLQALVSEPIKPVIHTVVMSN
                     AVHAYISQSDKGDLVIGAGIDQYTGYGQRGSYQLIEGTLQAIVEMFPVFSRVRMNRQW
                     GGIVDVSPDACPIISKTDVKGLYFNCGWGTGGFKATPGSGWVFAHTVANDQPHPVNAP
                     FSLDRFYTGHLIDEHGAAAVAH"
     gene            complement(12501..13883)
                     /locus_tag="VFR20_03135"
                     /pseudo
     CDS             complement(12501..13883)
                     /locus_tag="VFR20_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006480589.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
     gene            complement(14192..15250)
                     /locus_tag="VFR20_03140"
     CDS             complement(14192..15250)
                     /locus_tag="VFR20_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEU0229542.1"
                     /translation="MPDTGSATTGTRPVWFGILLLPDFTLTAFSGLVDMLRLASDEGD
                     NSRPVRCCWSLIGEGRKPVRASCGIETTPWEELGDPSRFDYVVVVGGLLRKGSEPSRA
                     TLDFIRAVGETKTTLVGICTGTFALMRAGVLARHRVCVSWFHYWDFLEQFPHVEPARL
                     VADRLFVVDGHRITCSGGRASIDVAAEILKRHIDVSTVQKALRILLVDRSETVNAPQP
                     HPPGLEPSAHPLVKRAMLLMEQHLSKALTVDEMAGKLNVSGRQFERIFKADTGKTPQA
                     FARALRLRVAAWSLLNTDKPISVVSTECGFADASHMGREFRQIYAMSPSGFRRKKGEI
                     QLGDVNYHEIFPNRTEFY"
     gene            complement(15421..16248)
                     /locus_tag="VFR20_03145"
                     /pseudo
     CDS             complement(15421..16248)
                     /locus_tag="VFR20_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004200833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     permease"
     gene            complement(16266..17444)
                     /locus_tag="VFR20_03150"
     CDS             complement(16266..17444)
                     /locus_tag="VFR20_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007745800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEU0229543.1"
                     /translation="MSTDKIVVENLYKIFGANPALAQELLSKGRTKDDIFRETGQVIG
                     VDNVSFSVSEGEIFVLMGLSGSGKSTLIRLINRLIEPTAGNVFVDGQNVSTMNRAQLT
                     ALRRRNMSMVFQSFALMPHRTVLENASFGLEVAGVAKKERERRAMETLEQVGLAAFAH
                     KRPFELSGGMQQRVGLARAFTVDPSLILMDEAFSALDPLKRREMQDVLLDLQREQRRT
                     IIFVSHDLEEALRIGSRIAIMKGGVVVQVGTPREVINHPADDYVRAFFNGVDTSRYLT
                     AADILEPDGIPVISYPYVSDKTEQVDIDDQGAFLLNENKQVHGFVANRDELERCKVGA
                     SRPLSSPVIPSSMPLNELMDVLMQSHRPLPVVNELGRYCGALCHATVLKTIAANRGGA
                     "
     gene            17818..19017
                     /gene="fdhA"
                     /locus_tag="VFR20_03155"
     CDS             17818..19017
                     /gene="fdhA"
                     /locus_tag="VFR20_03155"
                     /EC_number="1.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018467 - formaldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde dehydrogenase,
                     glutathione-independent"
                     /protein_id="HEU0229544.1"
                     /translation="MADNHGVVYIEPGKVEVQSIPFPKMTDPQGRKIEHGVILKVVST
                     NICGSDQHMVRGRTTAPAGLVLGHEITGEVIELGRDVERIKVGDLVSVPFNVACGRCR
                     CCLEQNTGVCLSVNPARPGGAYGYVDMGGWIGGQAEYVMVPYADFNLLRFPDRQQAMS
                     KIRDLTCLSDILPTGYHGAIMAGVGPGTTVYVAGAGPVGLASAASARLLGAAVVIVGD
                     VNPARLEHARKVGFETVDLSQDTPLGDQIADILGTPEVDCAVDAVGFEARGHGHTGSR
                     SEAPATVLNSLMAVTRAAGKIGIPGLYVTDDPGAADAAAKHGSLSVRFGLGWAKSHSF
                     FTGQTPVLKYNRALMQAILWDRINIAEIVGVQVITLNDAPKGYAEFDSGAPRKFVIDP
                     HQMLKAA"
     gene            complement(19024..19725)
                     /locus_tag="VFR20_03160"
     CDS             complement(19024..19725)
                     /locus_tag="VFR20_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229545.1"
                     /translation="MLVLGLALAGCASSSAPTTRGTPKDEGMSFDQLGQTDFDRTVTV
                     AMKQNLATLSALMEKLYKRNPVEWHKSHFPNLAAALAQGRQAIATGQVPPDLAGMRDI
                     QVLSVALDPGYRGDRVRAFIEGLANMIIEAHGGRTRFYATDRLDAQHVYNAARNVETA
                     AWMLATRRDASGRPLLLSNEMSVDGASNLSFERGFGELIGRLDLVASLLDENLRRIGI
                     NYVQGLLLFNFLPVR"
     gene            complement(19789..21258)
                     /locus_tag="VFR20_03165"
     CDS             complement(19789..21258)
                     /locus_tag="VFR20_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEU0229546.1"
                     /translation="MNARIPDDTASLALNPDQLQTFVDEKWDTGIVPELTDYIAIPAK
                     SPAFDPEWVANEHLERVVRNAAKWVEDQQVSGLKLEVVRLAGRTPVIFFDAPSTRSDS
                     GDTVLFYGHLDKQPEFMGWRNDLGPWTPKYEDGKLYGRGGADDGYAVYASLTAIAALD
                     KQGVGRPRCVGIIETCEESGSYDLLPYVDALRPRLGNVALVVCLDSGAGNYDQLWMTT
                     SLRGLVAGTLEVQVLDEGVHSGDASGVVPSSFRILRHLLDRLEDSATGRLLPQSFHCE
                     VPAERLDQIRLAAKLLGDDVWKRFPWSCGGDGGFVLPMTTDAEQALLNRHWRPTLSVT
                     GAEGLPPVASAGNVLRPRSAFKLSLRLPPLVDAVAASQELKALLEADAPYNAKVVFKP
                     DEGVASGWNAPESSAWLTSALDAASRQYFDAPCGYIGQGGTIPLMRLLQKGFPTSQFM
                     VCGVLGPRSNAHGPNEFLHVPFGKKLTASVAQTIAALPA"
     gene            21466..22170
                     /locus_tag="VFR20_03170"
     CDS             21466..22170
                     /locus_tag="VFR20_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0229547.1"
                     /translation="MSDLSAFPITKKWPARHPDRIQLYSLPTPNGVKVSIMLEETGLP
                     YEPHLVSFQTNDQTSPEFLSLNPNNKIPAIIDPAGPGGKPLPLFESGAILIYLADKSG
                     RFLPTDAAARYQAIQWLMFQMGGIGPMFGQVGFFNKFAGKSFEDKRPRDRYVAESRRL
                     LGVLDRQVASQDWIMGRDYTIVDIATWPWVRNLIGFYEAGDLVGIGDFPQVTRVLDTF
                     LARPAVQRGLNIPARD"
     gene            22240..22770
                     /locus_tag="VFR20_03175"
     CDS             22240..22770
                     /locus_tag="VFR20_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0229548.1"
                     /translation="MTHTAQSHPLTFRRLLAPDAPAFQQLRLESLHDHPEAFSATWEE
                     ERLQPLEWFAKRLENSVVLGAWRGDTLVGIAGLFVPLSPRQRHKNALWGFYVQPQARG
                     AGLAQDLLSRIIGQAATRAEEILLGVAASNSPALRLYKKAGFQEYGLERRALKMGDHY
                     DDVILMSLALPSARPT"
     gene            complement(22812..23240)
                     /locus_tag="VFR20_03180"
     CDS             complement(22812..23240)
                     /locus_tag="VFR20_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HEU0229549.1"
                     /translation="MDDPHDLQRFVNAQEPVIEQVFQELRAGRKQSHWMWFVFPQIQG
                     LGGSPMARRFAISSLDEARAYLRHPVLGERLRRCTRLVNAIEGCSLGQVMAYPDDLKF
                     RSCMTLFAQAGEDVQVFADALDKYCGGEPDEKTLAILGLR"
     gene            23375..24343
                     /locus_tag="VFR20_03185"
     CDS             23375..24343
                     /locus_tag="VFR20_03185"
                     /EC_number="4.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012339928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threo-3-hydroxy-L-aspartate ammonia-lyase"
                     /protein_id="HEU0229550.1"
                     /translation="MNTTILPSYGDVVAAAGRLDGQANHTPVMTSRALDEETGVRFYF
                     KCENLQRMGAFKFRGAFNALSRLSPEQRKAGVIAFSSGNHAQAVALAARILGMPATIV
                     MPQDAPAAKMAATKGYGGKVVLYDRYKEDREQIARDLADKQGLTLIPPYDHPDVIAGQ
                     GTAAKELLEEAGELNALFTPLGGGGLLAGSALSARALAPNCSVYGVEPEAGNDGQQSF
                     RSGAIVNIDPPRTIADGAQTQHLGGYTFPIIRRDVADVLTATDTQLVDCMRFFASRMK
                     ILVEPTGCLGFAAARHMAPQLKGQRIGVILSGGNVDLERFSELLRA"
     gene            complement(24321..25277)
                     /locus_tag="VFR20_03190"
     CDS             complement(24321..25277)
                     /locus_tag="VFR20_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEU0229551.1"
                     /translation="MALSIGFVVYPGFNLLDLSGPLGAFDTAARTSEEPVYSLRVLSE
                     AGGLVASSSGVEVVTSPLGAYAPDTVIVVGGRGVSLALESPSLVSFVQAAASRRLASV
                     CTGAFLLARAGVLDGRRATTHWRHAARLQRDYPRVRVEADRIFVRDGAVWSSAGITAG
                     IDLALALIEDDMGAQVARAVARDLVVYYRRPGGQSQFSTLQALDPPSDRLRQALAYAR
                     EHLHEALPVERLAEVACLSPRQFTRTFVAETGVTPAKAIERLRAEAARSRIEESREPL
                     EQVARGVGFSDTERMRRTFLRLFGHPPQALRRAARVMPAAAR"
     gene            25447..26130
                     /locus_tag="VFR20_03195"
     CDS             25447..26130
                     /locus_tag="VFR20_03195"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU0229552.1"
                     /translation="MADSLNIVFPIYPGVTHLDFTGPHQVLARLPGTRITVASAGGQP
                     VAADGFNFNGLARLEDVDHCDVLCVPGGHGCIAVLQDPVYMAAIRRLATSARYVTSVC
                     TGSLILGAAGLLVGKRAACHWAWRDLLAPLGAIPDPARVVRDGNILTGGGVTAGIDFA
                     FTLAAEIAGEEAAQTLQLGLEYAPAPPFNSGSPDSAPRAVLERVQALNAPQQAARRAA
                     IDQAARALG"
     gene            26325..27098
                     /locus_tag="VFR20_03200"
     CDS             26325..27098
                     /locus_tag="VFR20_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0229553.1"
                     /translation="MTQAHRDTVHSQFDPQANAYLQSAVHAAGPDLERAREIVGQAVS
                     AASATLLDVGCGAGHLSFALAPAVLGVAAVDPSPGMLATVAQAAVDRGLANVTTCQAS
                     AEQLPFDDGTFDIVATRYSAHHWLDLPRALKEMRRVAKPTGRVLIIDLEGDENALVDT
                     HLQAIELLRDLSHIRDRSPSEWGRLLADAGFTGVGHESWPTRLMFEPWVTRMRTAPER
                     VAMIRAIQQECPQEVRAALSIEEDGSFTARTGLWWGSAA"
     gene            complement(27150..27530)
                     /locus_tag="VFR20_03205"
     CDS             complement(27150..27530)
                     /locus_tag="VFR20_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU0229554.1"
                     /translation="MDRIRLDKWLWAARFYKTRSLAAHEIGAGRVQVDGQAVKPAREI
                     APGDQVSVRKEGSTVHVWVRALSTVRGPAPRASLLYEETPESIAARERAAEMKRLAPE
                     PARDVAAGRPTKRDRRLIDLMRGR"
     gene            complement(27595..28260)
                     /locus_tag="VFR20_03210"
     CDS             complement(27595..28260)
                     /locus_tag="VFR20_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229555.1"
                     /translation="MTQATTSLKPRATAKAANIVREYGPFEGVDAVHGVTSDGQRVWA
                     ATGARLVAIDPGSGTIERTLDCACDAGTAFDGTHLYQIAGDRIDKIDPSNGQVIGSIP
                     APAHGAVSGLAWAEGSLWVGQYRDRKIHRVDPATGDILRSIETNRFVTGVAWVDGELW
                     HGTWEDEQGGIQHIDPRSGTVLESLEMPCGIGVSGLEPDGAGGFYCGGGTSGKVRVVR
                     RPG"
     gene            complement(28307..29530)
                     /locus_tag="VFR20_03215"
     CDS             complement(28307..29530)
                     /locus_tag="VFR20_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0229556.1"
                     /translation="MDALITAAARALMNGDPLGALKRIALRDDPPALALRGIAMAQLG
                     EHVRARELLSRARRGFGAHEVLSRARCAVAEAEVALAARNLGGSLRSLAAAIATLEEH
                     ADPVNAMHARLIAVRWQVLTGRLREAETALSGLDMRDQPPSLVAAAELTAAELALRAL
                     RVEAGKAALVRARNAARKSGVAALLAEVAQAGAALEQPAARRVGHEGEQALLLGQVAE
                     LLESGTLVVDACRRRVGTGTAWCFLTRRPILFALVRALARVWPGDIERDALIHEVFRM
                     RRPDESHRVRLRVEMSRLRSVLKDLARIDATSGGFAIRPMDGRAVAVLAPPFEDDEQA
                     ALMALLSDGAAWSTSALSLALGASQRTIQRVLVDLESVGRVRSVGRARAQRWLSPPLA
                     GFTTILLLPAALPVG"
     gene            complement(29665..30693)
                     /locus_tag="VFR20_03220"
     CDS             complement(29665..30693)
                     /locus_tag="VFR20_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229557.1"
                     /translation="MSRVSAAVLAASLFTIPVALLPLPAAAAVSVGISVNVSPPALPV
                     YEQPAIPGPGYIWTPGYWAWSNLGYYYWVPGVWVLPPIVGVLWTPGWWGWSDGFYRWH
                     PGYWGPRVGFYGGINYGFGYFGTGYVGGYWRGRDFYYNRAVNHINVTNIRNVYVNKTV
                     INNVHINRTSYNGGRGGIVMRPTAAQRSYGNQHHYAPTSFQARQREMSERDRGQRFDM
                     NRGRPEAPARQYMAHPESRPNPGQPRQAGRVQPQQAGRPEQRLQQERRIEQRPARPEQ
                     SPRVNQPRPPVSQERAVTQRRNEYRNTPPQRPPRQNESQQRRQNNAERPHQDRRGGGR
                     PERREGDH"
     gene            31051..31842
                     /locus_tag="VFR20_03225"
     CDS             31051..31842
                     /locus_tag="VFR20_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU0229558.1"
                     /translation="MRLARPILILLAMLIASPALAKMIAQPVSWTLDGTHFNSVLVYD
                     NASQARRPGLVMVPNWSGINDIAVEKAKRIAGKDYVILLTDMYGGGIHPKNTEEAQAA
                     VQPLYADRNLMRARIRKAFSELKAQARQAPIDVSRLAAIGFCFGGAAVLDLARSGSDV
                     KAVIAFHGDLRTDNPALAKNIKARVLAINGADDTSTMPAVPAFTEEMRQSPADWQFVE
                     IGHVVHCFTETEATAKTGNCRYDAKAAARSYRMMRNWLRDSFKEG"
     gene            complement(31991..32605)
                     /locus_tag="VFR20_03230"
     CDS             complement(31991..32605)
                     /locus_tag="VFR20_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0229559.1"
                     /translation="MIRIENLSKRYGDHLVFQGLTRTFPPGCVALCEEDSTGKSSLLG
                     VIAGVLVPDAGDVWIDGYSLVREPKQAKARLAYVPENCLAFPLQTGRGLLEQVATEKN
                     TTLGDAVLDFAYELGLEPHLDKRFEQMSTGTRRKVFLTAAALGDPVAVIADGPSDGLD
                     ARARAVLAEQFKVWSRDRVVLFASHDRELVRACGASAINVSALC"
     gene            complement(32647..33993)
                     /locus_tag="VFR20_03235"
     CDS             complement(32647..33993)
                     /locus_tag="VFR20_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HEU0229560.1"
                     /translation="MKLDVRVCGRTVASLYRERDEYVLRYLPGTDPADFVSLAMPVRE
                     EPWRWPRELHPFFRQNLPEGYLLSVIREEFGPLLDGTDLSLLAVVGGMGIGRVTVTPE
                     GGALDADREPLQIERLLKAENTTEHFAGLVRRYARASISGMVPKFIAPEDGPGKRDHP
                     LGKPTLRTSRHIIKGSDDGTPYLGFNEFHTMRVLERLNVVPVAGMRMSEDGRVLVVDR
                     FDVDEHGEPTHGVEDACGLLGLPPHEKYATTIERVLKATRVYVPAKNVRSQLEQFGWH
                     LLTDYVVRNADCHAKNIALYYTSLKDVAYTPVYDVVTTQAYPRYAANPPGLPVDGRQT
                     WRVGKTLERFFKARLGIAPKQYAEMVERLCESAVDVGLEVIEAARNEPRWHTVAKHMV
                     HAWNEGMASLHDSKGSVAFQGLGDAIARAGFSEPKRPESARTVIGRSELLGVPRKR"
     gene            complement(33990..34253)
                     /locus_tag="VFR20_03240"
     CDS             complement(33990..34253)
                     /locus_tag="VFR20_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0229561.1"
                     /translation="MSTLADVANMLRRARQEAGLSQETLAQRAGVSRTTVARMETLAK
                     GDMSVSALVRLLEAAGYDIKLIKQGHVRTIEDILAEQRLGHEP"
     gene            complement(34497..35651)
                     /gene="ampC"
                     /locus_tag="VFR20_03245"
     CDS             complement(34497..35651)
                     /gene="ampC"
                     /locus_tag="VFR20_03245"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class C beta-lactamase"
                     /protein_id="HEU0229562.1"
                     /translation="MTVLKSLALGLTFLALSLQSAHAKALPFQELARQAFASAIKTYK
                     IPGLVVGVTYHGKHYFYSTGLASRADNRPVTPDTLFELGSISKVFTATLAALAEQCGK
                     LSLNDKAAHYLCADACRIGDDMTLMDLATHHSGGLPLQVPDDVKSVGELVTWLKTWRP
                     PQPGARSYSNISIGLLGYITAQALGMPYARAAETMLFPELGLTHTWINVPASAMKNYA
                     YGYDRKTDKAVRVTPGVLDAEAYGVKSSARDMVKYLDAELGNGVLSPELKAAIHRTQE
                     GRFKTAYFTQDMIWEQYPRPVSLKTLLLGNGYDFILHPQPVEKITPALPPQRNVLLDK
                     TGSTNGFGGYVAVLPGERLGIVVLANRNYPNEARVKATYALIEALLADRQ"
     gene            35747..36118
                     /locus_tag="VFR20_03250"
     CDS             35747..36118
                     /locus_tag="VFR20_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HEU0229563.1"
                     /translation="MGATSILALHLVVIAFNVAGCILIPVGALLSWRWVRGFWWRAAH
                     LASLAVVAAQALLGRACFLTIWQADLANHRHAARPLIASWIDRLIYLPLPIWVFATAY
                     VAIFIYVVALWVWVRPRRRRA"
     gene            complement(36122..36652)
                     /locus_tag="VFR20_03255"
     CDS             complement(36122..36652)
                     /locus_tag="VFR20_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229564.1"
                     /translation="MPLINPNPRASRLAWIPAHILIGVLLAVASQGASAQSNDARECR
                     AAGGTLLVGQVVSAPKFKRGRRKMGVELSHTHLKLQGNDGTTYDVAIDNVFASGYRPH
                     SRKVPEPLNTIVVGDKVEACGIPFRGGIHWVHNNCGDRPTRRDPNGWLKVVRADGSTG
                     PNLEGSQKYCGLWPRR"
     gene            complement(36822..37496)
                     /locus_tag="VFR20_03260"
     CDS             complement(36822..37496)
                     /locus_tag="VFR20_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HEU0229565.1"
                     /translation="MQYASPMRRLSAMALWLAAAATTAGLAQAAPAPAAAASQATATA
                     TAANPKLQALWQQVIQSKYIIQGASDATKKATVYAFFDPNCIFCHLSWRLSRHYYAEG
                     LQVRWIPIAFLMTDSAPKAAWVLLSADPSKALAEGEGGWKGLRDGGGAFNESLVTANI
                     QKTLDRNKKLFKDLGLTGTPGFVYKDPKGVVHVASGMSNKHLFANMTGLAYVKVDDPD
                     LKDIPE"
     gene            37712..38416
                     /locus_tag="VFR20_03265"
     CDS             37712..38416
                     /locus_tag="VFR20_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU0229566.1"
                     /translation="MTRVRVAGFTISLDGYGAGPNQDINNPLGIGGTELHQWLVPTHT
                     FQRALFGKDGGKTGVDDDFAARGFQNVGAWILGRNMFGPIRGGWPDTNWKGWWGDNPP
                     YHVPVFILTHHARPPIEMEGDTTFHFITGGIREALDRAREAAGGMDVRIGGGSGTIRQ
                     YLREGLIDELHIAIAPVLLGGGEALFEGIDLRALGYECVEFVASGKATHVVLRRQGHT
                     EARPAPQHPKPASAQL"
     gene            complement(38466..38729)
                     /locus_tag="VFR20_03270"
     CDS             complement(38466..38729)
                     /locus_tag="VFR20_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEU0229567.1"
                     /translation="MADRLLCWTDAAWDDYVYWQGQDRKTLKRINKLIDDVRRSPFEG
                     IGKPEALKENLSGFWSRRMDDTNRLVYAVDTATITIISCRYHY"
     gene            complement(38722..38976)
                     /locus_tag="VFR20_03275"
     CDS             complement(38722..38976)
                     /locus_tag="VFR20_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEU0229568.1"
                     /translation="MKVVSFSEARNSLKTVLDAVVNDADTTIITRRDTADAVVMSLDY
                     YNSLMETVHLLRSPANVEHLSRSIKQYRAGKAKPQELING"
     gene            complement(39089..41566)
                     /gene="gyrB"
                     /locus_tag="VFR20_03280"
     CDS             complement(39089..41566)
                     /gene="gyrB"
                     /locus_tag="VFR20_03280"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU0229569.1"
                     /translation="MSDQNTQTQSTGYSADSIKMLKGLEAVRKRPGMYIGDTSDGTGL
                     HHMVFEVVDNAIDEALAGYCDDIVVMIHSDDSISVTDNGRGIPTGIHKDDEYHRSAAE
                     IVMTELHAGGKFDHNSYKVSGGLHGVGVSCVNALSEWLRLTIWREGKTHEIEFRQGER
                     VAPLAETGPTEKRGTRVQFLADPVIFEKIEYHYEILAKRLRELSFLNNGVKIRLIDER
                     QGKEESFAFLGGVKGFVEYINRAKTVLHSNVFAVSTESSVGGTTVGVDVAMQWNDSYA
                     ETVLCFTNNIPQRDGGSHLTGLRAAMTRIINKYIADNELAKKAKVDTSGDDMREGLTC
                     VLSVKVPEPKFSSQTKDKLVSSEVRPAVEDAVARTLETWLLENPNDAKALCGKIIDAA
                     RAREAARKAREMTRRKSVLEGAGLPGKLADCQEKDPALCELYIVEGDSAGGSAKQGRD
                     RKFQAILPLRGKVLNVEKARFDRLISSEQITTLITALGTSIGPDFNLEKLRYHRIIIM
                     TDADVDGAHIRTLLLTLFYRQMPSMVEQGHVYIAQPPLYKVKVGRDERYLKDDQEEAQ
                     YMLQLALKEAELTPTEGANPITGDALAELARQYILADSVINRLSRTLDLKALSAMAEG
                     VEIRLDTEENARLSAERLQEVLDDPALPGSIIVTSEPAATTATNELGEPIPAWRVVVK
                     RLHHGNTRVSVFDRPFVRGADYATLARAAKTFLGLIGPGALIVRGTGDKRKEKYVADF
                     REVMKWLHNEAERGVSKQRYKGLGEMNPDQLWETTMDPTARRLLRVQIEDAIAADEVF
                     TTLMGDNVEPRRAFIEAHALQAGNIDV"
     gene            complement(41659..42765)
                     /gene="dnaN"
                     /locus_tag="VFR20_03285"
     CDS             complement(41659..42765)
                     /gene="dnaN"
                     /locus_tag="VFR20_03285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU0229570.1"
                     /translation="MQLLQTTRDALLKPLTTVAGIVERRNTLPILANILLRKEGESVT
                     FIATDIEVQISTQAGIGTGNGAEATTVAARKLLDILRALPDSDVKFELSGAKLLVQSG
                     KSRFSLQTMSASDFPAVSQPQTWDVSFSLPQSKLKHLFNMVHFAMAQQDIRYYLNGLL
                     FVFEPGHVRAVATDGHRLAHTGTAVEGITARHDVIVPRKTVLEMQRLLGDSEDPVSVD
                     VAQGQIRFRFGDVELVSKLVEGKFPDFTRVIPTNYTRHFSINREVLQGSLQRAAILTT
                     DKLKGVRVQLAQNQLTISSSNTEQEEALEELEIDYGHEPLDVGFNVHYLLDVLGNVKT
                     ETVQWSVQPDVNASVLITLPGDDQFKYVVMPMRI"
     gene            complement(42768..44177)
                     /gene="dnaA"
                     /locus_tag="VFR20_03290"
     CDS             complement(42768..44177)
                     /gene="dnaA"
                     /locus_tag="VFR20_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU0229571.1"
                     /translation="MKDFWQTCLQRLEQELPPQQISAWIRPLVPVAFDEAQAVLRVSA
                     PNRFKLDWVRRNYAGQIASLAAEWYGRPVEVEFELASAPAGAARPVAARPADAPAAIA
                     TPPAGADPAPATTPVPTVKVVTQPGQDRSRLNLDLTFDNFVTGKANQLARAAALQVAE
                     NPGVSYNPLFLYGGVGLGKTHLIHAIGNALLRDSADTRVRYVHADQYVSDVVKAYQRK
                     AFDEFKRYYHSLDLLLIDDIQFFAGKNRTQEEFFYAFEAMVAQRKQIIITSDTYPKEL
                     ANIDSRLISRFDSGLTVAIEPPELEMRVAILLRKAETEGITMPEEVAFFIAKHLRSNV
                     RELEGALRKVSAYARFHGRQVLTVDVCKDALKDLLSVSNGQITVENIQKTVADFYKIK
                     VADMYSKRRPANIALPRQVAMYLAKELTQKSLPEIGDLFGGRDHTTVLHAVRKITGAR
                     STQSELNHALHVLEQTLKG"
     gene            44628..44762
                     /gene="rpmH"
                     /locus_tag="VFR20_03295"
     CDS             44628..44762
                     /gene="rpmH"
                     /locus_tag="VFR20_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU0229572.1"
                     /translation="MKRTYQPSVTRRKRTHGFRVRMKTRSGRAVLNARRAKGRKRLAV
                     "
     gene            44813..45175
                     /gene="rnpA"
                     /locus_tag="VFR20_03300"
     CDS             44813..45175
                     /gene="rnpA"
                     /locus_tag="VFR20_03300"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU0229573.1"
                     /translation="MSASFPATARLHRPSEYALALKGKRVARGALLVVTTPRQVSDEA
                     VPVRLGLIVAKRFAARAVTRNTIKRVIRETFRRKQHELPQRDLVFRLHSKVQDLSLTE
                     LRTLIRAETEFLLERARS"
     gene            45172..45417
                     /gene="yidD"
                     /locus_tag="VFR20_03305"
     CDS             45172..45417
                     /gene="yidD"
                     /locus_tag="VFR20_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU0229574.1"
                     /translation="MIKYLLIAPIRFYRYFISPLLGRNCRFTPTCSAYAIQAIQTHGA
                     IAGLWLAIKRIGRCHPWCQGGYDPVPDVEHKWGHKCH"
     gene            45542..>46728
                     /gene="yidC"
                     /locus_tag="VFR20_03310"
     CDS             45542..>46728
                     /gene="yidC"
                     /locus_tag="VFR20_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU0229575.1"
                     /translation="MDIRRTVLWMIFSFSLLLLWNNWQVHNGQPSLFGLGQTSTHQAA
                     KPAVKASSTPVADANVPASAPGEAPAAKGNAAVPGNKAAPVAPSQKVQVRTDVYDLTF
                     DTKGAQLIGAKLLKYKAVDNPQQPFTLLDDSKSLTYLVQSGVVGAPNGQSYPTHLTPF
                     KLVSTDTTMTGDTLNVVFEATSDNVKVVRTYTLRKGSYDIGVKNAIYNLGTEPISPSL
                     YLQIVRDDSDPASASHFTHAYTGTAVYSSEDKFQKATFSDIQKGKDQYIKKANNGWIA
                     MVQHYFATAWIPPEGVVRTNQVAHLSNGLYAIRSILPVGTIAPGQDKVTTAQLWTGPQ
                     DQKAMGAVAPGLDLVVDYGWLTIVAKPLFQLMSLLHSLLGNWGWTIVALTLLIKAVFY
                     PLSA"
BASE COUNT         8939 a        15608 c        13733 g         8448 t
ORIGIN      
        1 cttgaaaacg acaccggcga cgtggcgcgc cgcctgggcg acgaggccac cttccagatt
       61 atggccgaac gcctcatcaa cacactgaat gggctcagct tcaaccgtat tgtcaccgcc
      121 gacccgcacg tactgcactg cctgcgcaac gaatacccgg cattcggcgg gcattacccg
      181 gtcattcatc actcggcact gctggacgac ctcatccgcg cacaagccat tgcgccgcgc
      241 gcccttgaac tcgaccacac gctgacctac cacgacccct gctatctggc ccgctacaac
      301 ggcgaaaccg aggcgccgcg ccgcgtcctc gaggcattgg gcatcaaggt gcgggaaatg
      361 acgcgcaacg gcttgaacag ccgctgctgc ggcggcggcg gcggtgcccc gctgaccgac
      421 atccccggca agcgacgcat tcccgacatg cgcatggacg acgcgcgcga catcggcgca
      481 cagaccgtgg ccgtcgcctg ccccggctgc accgccatgc tcgaaggcgt tgtcggcccc
      541 cgacccgatg tcctggacat cgccgagctc gtcgaactgt cactggggga agcggcatga
      601 tcacgcttgc acggccgccc gaaggatgtg atccctccca cggggcggac ggcgtgcgac
      661 gccaggaggg tcgttccatc gccttgtccg cccggaaggc cgtgtcatga acacccgtcg
      721 ccgcgtcgat ccgcgccgcc cgttcatcgt cacttcgctt ggccgcaggc gttacgccat
      781 cggccagggt gcgatatcag gcaacaacat aacgccagac acgccacaga cacctcacgc
      841 cccccacgac acgccgctgc ggatccatgc gccaaccgac caccacatca tggtggtggc
      901 ccacagcgaa cggggcgtgc tgccgtcggc ggcccaacaa gccgttgccg ccgccgcgct
      961 gctggccgac gcgcgctgcg ccgtgctgtt ggtcgtgttc ggtcctcttg cggaaagcgc
     1021 ggcggcttgc ggcgccgacc aggtcattgt cctggatggc gaaaatcgct ttgaacccga
     1081 ccttgcgcta cgcgagctgg accggctgcg acaacattac cacccggccc gcatcgtgat
     1141 ggccgacgac ggagtaccga ccggtgatct gggccgacgc ctcgcggcgc aacacggtct
     1201 ttccatcgcg acacatgtcg tggaactcac atccgacacc gcgacggtct atcagcgaca
     1261 ccggtccgcc tatgcgcggc gcgccctgcc ggacgtcgtc ctgctcgatc ctggctgcgt
     1321 cgacatgcgc ctgccattcc aggggcgcgg cgaaatcgtg gactgggcgc ccgaagatgg
     1381 cgtggcgtct cattacacgg accatggtgt ctcggccgtc ccggcgtcgc atctggccct
     1441 ggaggaagcg gacctgatcg tctccgccgg caacggcgtg cgcgacctgg acggcttcca
     1501 tgccctggcg gacgcgctag gcgccgcagt gggcgcgagc cgcgtggctg tcgacgacgg
     1561 gcgtttcacg cgcgcgcagc aggtcggcgc cacgggcacg accgtgacgt ccagcgtcta
     1621 tatcgccctg ggcatttccg gcgccgtgca gcacctgcag ggcatcaagg attgccgcca
     1681 cgtcatcgcc gtcaacaccg atgccagcgc gcccatgatc aaacgggccg atctcagcat
     1741 catcgacgat gcgcaggcca tcatgcgcgc actgaccacc gaggtccacg ccgcgcgcaa
     1801 cgcggccgac ggagcgaccc catgaccgac gacgaacatc cactccgcat tgtcgtgttg
     1861 gtgtccgagg gccggcaccc ggtctcgggc gtgtcgcgct ggagccgcaa cgacgcccga
     1921 gccttgagcc tggcctattc ggtggcgtcg ggcaagccca ccgtgcaagt gctgcacgcc
     1981 ggtcatgact cccccgccac gcgggattat ctggcgcttg gcgtcaacca cctggacatc
     2041 gtgccggttg ctgaaggcgt ggatgtggtg gatgcgctgg aagcgcatgt gcacaatgcc
     2101 gacctgatcc tctgcggact gtgcgccgaa ggccaggaag acagcggcat ggtgccttac
     2161 ctgctggcgc aacgtcttgg gctgccactg ctccccagcg cgcttgacct ggctatcgac
     2221 gcgggcggca tcaccatcgg gcaattcctg cccaagggcc gccgtcgtcg tgtcacaaca
     2281 acgctgccat gcgtggtcac cgtccacccc atggcccccc tgacgctgaa atacgcctat
     2341 gccggcacgc gcaccggcac gatcaccacg ctcccgcaca cacccatcca cgcgaccggc
     2401 caacgcggca tggactggac cgtgtccccc atcaacgcgc gccccatcaa gctggcggcg
     2461 catgaaagac gttccggcca tgcccgcatg ctttcggcga ccacgaccga gagccgcgcg
     2521 ggggtggtcg tggatcaagg cagcgcgcag gaaaaagcga cagctgtcat cacctacttg
     2581 cgcaagcatg gattgattga ttactgaggg catgatgaat gtcatacgca tggcggtcga
     2641 aaccgacaaa ctgaagatct cgccggacac ggccgcgctg atcgcgcgac gcccgccagg
     2701 ccggccgctg gaagcaccgt tctacaccag ccgcaagatc ttcgagatcg acatgaagct
     2761 gattttcggc cagcactgga tctacgtggc caccgaggcc gagatccccg agcccgggga
     2821 ttacgtgacc atagacctgt tgaagaattc catcgttctg gtccgtgatg acaatggcga
     2881 cgtcaaggca ttccacaacg tgtgccgcca tcgcggatcc cgcctgtgca atgagcacac
     2941 cggatcggtg ggcaacctgg tctgccccta tcaccagtgg acgtacgacc tggaaggcaa
     3001 actgctcttc gccgaacaca tgggcgagga ttttgatcgc gacgcgcacg gactgcggcc
     3061 ggtacacctg cggaatctgg ccggcctgct gttcatctgc ctggccgaca cgccccccga
     3121 cgatttcgag gcgatgcgcg ccgcgatgga accttatatc cgcccgcacc aactccacat
     3181 gacaaaagtg gcggcccagg tcgatatcgt cgaggaatgc aactggaagc tcaccatcga
     3241 gaacaaccgg gagtgctacc actgcgcggc caatcaccct gagctcaccg cctcgatctt
     3301 cgaatacggt ttcggctacc aagcctcgcc gtccaaccag gcgcaactca cggcctacga
     3361 caagctggtg gcgacccgca ccgccgactg ggaaggcaca tgcagcttgc cgtccgaaga
     3421 aatcgagcac ctggacgacc gcaccaccgg tttccgcacc caacgcctgc cgctggacca
     3481 aggcggcgaa tcggaaaccc tgaacgccaa ggtggcctgc agaaaactgc tgggtgactt
     3541 tcaacgtgcg gacctgggcg ccctgtcatt ctggacgcaa cccaattcgt ggcaccactt
     3601 catgagcgac cacattgtca cgttccaggt gttccccctg tccgagaacc gcacactggt
     3661 gcgctccacg tggctggtgc acaaggacgc tgtcgaaaac acagactaca cactggacaa
     3721 cctgaccgcc gtatggaacg cgaccaatgc ccaggaccgt cacttggtgg aaatttcgca
     3781 gaggggaata gaaagcgacg cctacgaacc gggcatgtac tcaccctatg cggaggtcta
     3841 tgtcgagaag ttctgccgct ggtacataga ccggctgacg gctctgacca cagcgtaacc
     3901 aacatcaccg cgggagcaca acatggacgt cagcgcgaat cgcatcaacc aggccgggtt
     3961 ctgggagcgg attccgccac aatggaatgg tgaagaggaa gaggcactga tctgctgcac
     4021 catcatcgac gaaacgcacg acgtcaagac gttcacgttc cgcacgccaa aaggcagcct
     4081 gtttcatttt gctcccgggc agtttctgac cctggagctg gaaatagacg gcgtgcaggt
     4141 gaaccgttgc tacaccatcg cttcggcgcc gacccgcccg cacacggtaa gcatcaccgt
     4201 caagcgcacg cctggcggcg tggtgtccaa ctggctgcat gaccacctga aggtgggcga
     4261 ccgggtgcgt gtcctgcctc ccggcggcac attcacgtgc gccgattttc cggcgaagaa
     4321 gtacctgttc ctgtcggcgg ggtccggcat cacaccgctg atgtccatga cgcgcacgca
     4381 tttcgacctg gccgatgatc gagacatcgt gttcctgcac agtgcccgca cacccgacga
     4441 catcatcttc gaacaggaac tgcgccgcct ggcctacacc cagcagaact tccgcacgta
     4501 cttcgtctgc gaagtcaaag gcacgcgtcg tgactggaac ggtgtgaacg gctacctgtc
     4561 tctggcgctg ctgcagttga gcactccgga tttcctggaa cgtgaggtgt tcatttgcgg
     4621 ccccgagccc tacatgcggt cggtgaaggg catgctgggc gacgcggggt ttccgatgga
     4681 ccatttccat caggaaagct tttcgtttga aacgctgcaa gacacacggg cggccacgga
     4741 gcacgaggaa caggccgccc cacccgtcga cgcgacggac atccagatgt tcaccgtcaa
     4801 attccaaaag agccagcgcg aaatcacctg tgtgtccaca cagagcgtgt tggacgccgc
     4861 cagacaggct ggcgtgcgcc tggcctcgtc gtgcacacag ggcatgtgcg gcacctgcaa
     4921 ggtcaagctc atctccggcc aggtgcacat ggaacacaag ggtggcattc gccaacggga
     4981 aatcgaccag ggttatgccc tgctatgctg cagccggcca ctgagcgatc tggtcatcga
     5041 taaataaaaa cacaacggcc cggcatcgcg tatctgcaat ggcgggccgt cggcgcttcg
     5101 agggacatcg aagcgcgtgt gctgcggatt cttacttgcc tacccagcca ttgacctcgg
     5161 cttcattggc ggcaacccac ttcttggcgg catcagacgg cttggtgcca tccttgatgt
     5221 caaccatgac tttcgcgatc tgaccgggct tccactggaa tttcttcagg aaggccacga
     5281 cctcgggggc tttcttctcg agcttgggat tgatcatggc atagacgttc tcggactcgc
     5341 caaacacctt cttgggatcg gacaggaaac gcagtttgta gcggccgaac atccagtgcg
     5401 gctgccaacc cgtgaccacg atgggcttct tggcccggat ggcgcgatcg agcgccactg
     5461 ccatgccggc gcctgagctg gccgccaggg tgaagcccag gtcatacgct ttgatggcct
     5521 tttgcgcttt gtccatcaca ccggccccgg cgtcgatacc cgtgatgaca ccgttgtagt
     5581 tcgccttgta cttcttcaga tcggcaacgg tcttcgcctt gacgtagtcc ggcacgacca
     5641 ggccgatctt ggcgtctttt tccacaatgc cttcgagcac aacgtctttc ttgaatttct
     5701 tgtagtactg ggcatgcgtg accggcagcc atgcggacag gctggtgtcc aggtcaccgc
     5761 gcgcaacgcc cgaccacatg atgcccgccg agaccgccac cagcttgacc ggataatgca
     5821 gcttctcgcg gatgatggca gccgcggttt ccgacgtcgc cacgctgtcg gcccaaccat
     5881 tgacatagcc gatcttgatc tcgggcttgt cggccgcctg ggcgaccgac gcgataccca
     5941 gcgtggcggc cagacccagt gcggtgaata gagagctcaa tttcatatgt cttccttttg
     6001 ttgaaagcct gacggctttt tttgaaaaaa aatgcccacc catcttttcc atgactactg
     6061 cgcaaggcag ctggaagaag aattctgata aagggatttg gtgtgaggaa agcgccaacc
     6121 acgctcagaa ctgggggtca caccgaacag cttacgatag gtcttgctga agtggcaagc
     6181 cgactggaac ccacacgcca ccgtgatgtt catcactgac agccctgttt gtgtcagaag
     6241 ctcccgtgcg cgctgcaatc ggagattcaa ataatattga gtcggcgtca caccaagatg
     6301 gtcccgaaac atgcgctgta tctggcgttg cgacgaatgc gacaactggg ccagttcccc
     6361 cagggacaat ggctcttcga tgttcgcctc catcagggtg gccacttcca tcagcgtctc
     6421 gcgcaccgtg cccactcttg ctgaaagcgg tacacgttga cgatcccggg cgtcgcgtat
     6481 gtgcgcgacg atgaactggc tggctatgga tgaccgaata tcacgcccca ccttgggaga
     6541 tatcagatgc agcatcatgt ccaatggcgc cgcgcctccc gtacaggtga cacggtttct
     6601 atccaccacg aacacttcct cctcgaagcc caccgacggg aactcctgac gcaacgccgt
     6661 caggttctcc caatgcgtcg aacaacggta gccattgagc aggcccgccc gcgccagggc
     6721 atacgcaccg gtgcaaagac tgcccagcaa cacaccgcgg gccgccagca tgcgcagcat
     6781 ggacagtgtc tgtgggtcac aggcggcctt tacatctatg cccccgcaaa cgaaaacaat
     6841 atccggaata tccagcgggt caagcggacg ggccgtcacg gaaatgccat tgctggccac
     6901 cacgtgatcg cgatcgggat gcagcatcga ccatcgatac gtctgcgttc cagacagatg
     6961 attggccatt ctcagcacct ccacggcgct ggaaaaagac agcagcgtga aagagggcaa
     7021 taacacgaag ccaaaatgcg tgcagtcact gatggcgagc cgactcattg tattcacccg
     7081 gaaatagtgg aaatcaatac atctgaaacc ctgaaacccg ttgctttgct tcctgttgcc
     7141 ttccttccgc cgcaatgccc ctgtgcatgc gcatccccgg ccacaccact gaagccaggc
     7201 ccgcgcaggc ctggctaggg gccatattcg gccgacgcat cgaccaacat tcgtgtcgcg
     7261 tagtccgcga agctgcgccg cacgatcaga tgccacgtcc cgtcgaccag cttgcgcagc
     7321 gcgatcgccg tcttgaaaaa atgtgattgc gcgcattgcc ccggcttgaa cacacgagga
     7381 tgaagatcaa gcgggcaccc cttgttcaga acgtcggaca ccctggaacc cgacagccgc
     7441 aacgtggtgt agccgctgct gacgtcgacg gtggtcgcga acaggtcgcc gaagcgaacg
     7501 cgtaaatcgt ggtcgaggcg cgcgggcatg gggtcgtgcg attgcgccag ccattcgtcg
     7561 ggccccagcc accacaagac atagccaggt ccctcggaca cggtattggc cctcacaggc
     7621 aagtcgacgc cgatgacttc gctgatcgcg cgccgaaagt cggggtccga cgcattgccg
     7681 cggatattgt tcagctccag gaagggcagt tcgcacagcc gtatctgttc gctcagggtg
     7741 gcggagccgc cgtccaggag atccccggcg ccgaccagcg gggactcttg tcgtagttca
     7801 gtctgcattc tggcgcgttc cttctggatc gtagaaaacg gggctgcata cggtcgccga
     7861 cgcactgacc ccctcggaca gggacaccgt gaccttttct ccctggcgcc gcaggccgtc
     7921 gcgcaatagc gccaaggcga tggtgcggcc cagcgtcggg cttctgtagc tcgaggtgac
     7981 atgccccatc atcggtaccg gcctctcatt cgactggcgc gcgatgatct gcgccccctc
     8041 tggcaacacc aaggccggat catcggccag caggccgacg aattgtttgc gatccggacc
     8101 ggcggtatgc gcgcgcgtga gcgaacgctt gccgatgaag tccttggtct tggccaccaa
     8161 cccgcccatg cccagatcga acggcgacac ggaaccatcc gtatcctggc ccacgatgat
     8221 gtagcctttc tccgcgcgca acacatgcat ggcttcggtg ccgtacggtg ttatgccgaa
     8281 ctcgcgaccc gcctccatca gcaggtccca gacggcttgg ccatggttgg ccggcacgtt
     8341 cacctcgtat gaacgctcac ccgagaagct gatgcgcatc acacgcgcga agacaccggc
     8401 cacggcgcca tcccgaaaac tcatgaacgg aaaggcctcg tccgagaaat cgatgccggc
     8461 gcacaccttg ccaaccacct tgcgcgaatg cgggcccacc acggcgaacg tcgcgaaatg
     8521 gtctgtgacg gacgtcatgt gcactttcag atgcggccat tccgtctgat gccagcgctc
     8581 catccaggcc atcacgcgcg ccgcaccgcc cgacgtcgtg ctgagcagaa aatgatcctg
     8641 gccaaggcga atgttcacgc catcgtcgaa caccatgccg ttctcatcca gcatcaggcc
     8701 gtagcggcac ttgccgacac ccagcttggt ccaggagttg gtgtagatgc ggttcaggaa
     8761 ttcgacagtg tccggcccct ggatatcaat cttccccagc gtggaggaat ccatgatccc
     8821 cacgctgcgc cgcaccgcca gacattcccg tgccaccgcg gcatgcaagt cctcaccttc
     8881 catcggatag aaccacgggc gtttccaatt gccgacgtcc tcgaaccgcg cccggtgccg
     8941 cgcgtgccac tcgtgcagac aggtcttgcg tatcggatcc aggaaatcgc ccagctcccg
     9001 accggccacg acgccgaacg tcacgggtgt gtagttcgga cggaacgtgg ttgttccggt
     9061 ttcgggaatg gtctgtccga gcagatcggc cacgatggcc atgccgttga tattgcccag
     9121 cttgccttgg tcggtgccaa accccagggc ggtataacgc ttgacgtgct cgatggactc
     9181 atacccttca cgcaccgcca gatggatgtc ggacaccgcc acgtcattct gatagtcgac
     9241 gaactgcttg gccgcgcggc caacctggtc tttgtgaccg gcgaaccaga ccggtacgga
     9301 cggatcttcg cgcagagccg gcgtgcgcca ggcgggggtg ggcacggcct cgatccctgc
     9361 gcgacccagg gcggcacgcg ccatggccaa gccgccttcg agcgcctgct caagcaggaa
     9421 gtcgccattc gccgcgcccg cgctgaaggt cggctgcgcc ggattccctg gaacaaagca
     9481 ggccttgtcg tcatcccagc gcgccttccc gcccgactgg gcaaacagat gcagcacagg
     9541 attccaccca ccggacatgc caagcagatc gcaggcatgg tcggcaatga tgtctccggg
     9601 cacgccattt ttatacgacg ctatgcgcac accgctcaca tgacgcgtgc cgcgggcctc
     9661 gatcacgatc gcttcattca tgaccgtgac acctttgcca cgggccagtg tcggcaacgt
     9721 gccgccggac gcgggccggg catccaccac ctttacctgt gcgccatgac gcgccagatc
     9781 gagcgccgtg cggtaggcgt cgtcgttgtt ggtgaacacc accgcccgct ccccgggcag
     9841 cacgccatac tcattgatat aggtggaaat ggccgacgcc agcatgacgc ccggcagatc
     9901 gttgttgccg aacaccatgg gcctctcgtg cgcgccggtc gccagcacca cggcgcgggc
     9961 acggaccttc aacagacgct cccgaacgcc tcgccggctg tgcaccggca aatggtccgt
    10021 caaacggtgg catacggtca ccaggttgtg gtcgtgatgc ccgaaggccg tggcccgcgt
    10081 caggaacgtg acgtcgggac tggcctccag ctcggcaacc cgctccgcca cccaggccga
    10141 cgcgggcgcg ccgtcgattt cggcctcttc gcgcagcaga gcgccgcctg cccgggggcg
    10201 gtcctccacc agcatcactc gcgccccgga ccgcatggcc aagtgcgccg cggctaaccc
    10261 cgccgggccg gcccccacca ccagcacatc gcaatgacaa aaggattttt cgtaacgatc
    10321 gcgatcgggg agatgtggcg ccgtgcccaa cccggccgct tcgcggatgc gctcttcgta
    10381 tcgaggccac agtttgcgcg gccacatgaa cgtcttgtaa tagaacccgg cgggaatgaa
    10441 gcgcgccagt ttctgcagga aggccaggcg gtcgccctca atgctcggcg aggcgttcac
    10501 gctgctcgca accaggccgc tgtagagctc gacctcggtg gcgcgtgggt tgggaatcgt
    10561 gtaggcgcct tcctcgagct gaacaatggc gttgggttct tccacaccgg cggtcagaat
    10621 gcctcgcggc cggtggtatt tccagctgcg cgcgacgaag tgcacaccct gcgccagaag
    10681 cgccgaagcc agcgtgtccc cctgaaagcc gtgatacgtc ttgccgttga atgaaaattc
    10741 cacggtttgg gaacgatcca cccgtcctgt tgattcgagg cgacgggatc cgccgggttg
    10801 atggcgcgtg cggccttgcg cggcgccacc atcacgggaa ggcgcgcgct cgtcggacga
    10861 ccgggtcgaa tcggccattg agcggccatc ccgtcgttgc gcaagattct cgccggacgc
    10921 gctcatgcct gctccccgct gttgggcatc ggaaattttt catagttgcc gaactcgtag
    10981 gtgacggtgt tccgctccac tttgaaccag cgccggcacc cttgtgcatg cacccactgc
    11041 tcatgatgca cgccaagcgt gttggtgcgc atgaacacgt aatcacccca ttcccggtcg
    11101 ctcagattct cgccatccac ggggcgtgcg atatccgcct cgccaccaca gtggaactcg
    11161 gtttcggcgc gcttgccgca ccaggggcat tcaatcaaca gcatgcttgg actccgaaat
    11221 tttcagggcc tgccaggcct cacgtcaatc aatgggcgac cgcggcggca ccatgttcat
    11281 cgatcagatg gccggtatag aaccgatcca gagagaacgg cgcattgacc gggtgcggct
    11341 gatcgttggc caccgtgtgc gcaaacaccc aacccgatcc cggcgtggcc ttgaacccgc
    11401 ccgtgcccca gccacaattg aaataaaggc ctttcacgtc tgtcttggaa atgatggggc
    11461 aggcgtcagg cgagacgtcg actatcccgc cccactggcg gttcatcctc acacggctga
    11521 acacgggaaa catttcgaca atggcctgca acgttccctc tatcagttga tagcttccgc
    11581 gctgcccata ccccgtgtac tggtctatgc cggcaccgat caccagatcg cccttgtccg
    11641 actggctgat gtaggcatgc acggcgttgg acatgaccac agtgtggatg acgggtttga
    11701 tgggttcgga caccagcgcc tgcaacgggt ggctttccag tggcagacgg atgcccgcca
    11761 tggacgccag cacactggaa tggcccgccg tcaccaccgc gaccttggcc gccttgatga
    11821 atcccttgcc cgtgtccacg ccggtgaccg cgccgttctc gcgccgtatt cccgtgaccg
    11881 ggcagttctg gatgatgtcc accccgtact tgtcggcgcc gcgcgcgaac ccccacgcca
    11941 ccgcatcgtg acgcgccacg ccgccacgcc gctggaaaga cgcccccatc acgggatatc
    12001 gactgttcag attgatgatg gggacgattt ccttgatctg cgcgggcgtg atgaactccg
    12061 catccacccc gttgagacga ttggcgttca cgcggcgctg ggtgtcccgg acttcctgca
    12121 gcgtatgcgc cagattcatg acgccgcgct ggctgaacat cacgttgtag ttcaggtcct
    12181 gggacaagcc ctcccatagc ttcatcgcgg tttcgtacaa ctgcgccgac tcgtcccaca
    12241 ggtaattgga gcgcacgatg gtcgtgttgc gcgccgtgtt gccgccaccc agccagcctt
    12301 tctcaagcac cgccacattg cgcacgccgt gttctttggc caggtaatac gccgtggcca
    12361 gcccatgacc gcccccaccc acgatgacca cgtcatattc gcgccgtggc tcggggctgc
    12421 gccaggcgcg ctgccagttc cggttgtagg aaagcccgtt tcgcaccagg ctgaaaatag
    12481 aatatcggtt catgttcgac tcagcactca atcacgttca cggccaggcc gccacgcgag
    12541 gtttccttgt atttggtctt catgtcggcc cccgtctgca tcatggtctt gatcaccttg
    12601 tccagcgaca cgtaatgtgt gccgtcgccg cgcatcgcca ggcgcgcggc attgatagcc
    12661 ttgaccgcgc ccatggcgtt gcgctcgaca cagggaatct gaaccaggcc gccgaccgga
    12721 tcgcaggtca tgcccaggtt gtgttccatg ccgatttcgg cggcgttttc tatctgcttg
    12781 ttggtgcccc ccatgacggc cgccagcgcg gccgcagcca tcgagcaggc cacgcccacc
    12841 tcgccctggc agcccacctc ggctccggaa atggacgcgt tttccttgta cagaatgcca
    12901 atggccgcgg ccgtcaacaa aaattcaacc accccatcgt catcggcgcc tggcacgaat
    12961 ttcctgtaat agtgcagcac cgccggcaag agaccggccg cgccgttggt gggcgccgtc
    13021 acgacgcgcc caccaacggc gttctcttcg ttgaccgcca tcgcatacag gctcacccag
    13081 tccagcacgg tgagcgggtc ctgcaacgac gcctcaggct gctcgcacag cgcccggtac
    13141 tgcgccgccg cgcgccgctt catcttcaaa gggccgggca ggcacccctg cgccgcgcac
    13201 ccgcgcttca cgcagtcagt catcacgcgc cagatgttca acaccccatc gcgaacctcg
    13261 ttcggcgtgc gccaggcctt ctcgttttcc atcatcagtc cggcgatcgt caggccagcc
    13321 tgatcggcca tgcgcagcag ctcgtccgcg ttgcgaaacg ggtaggccag ggtcaccccg
    13381 tcattggccg cgttgacccg cacgccgtgg cagttcatga cgaacccgcc gccaatggag
    13441 taatactctt ttttcatgag gacggcaccg gcaccgtcgg tcgccacgaa acgcatgccg
    13501 ttcgagtgac ggggcagcac gccgcgattg aacgccacat cacgtttttc gacgaacccc
    13561 accggcaccc gacccagcag attgatggtc ccgccctctc gaatgcccgc cacgacaccc
    13621 ggcacaagat ccggatcaat ggtgtcgggc agatgcccgc acagccccag caacaccgcc
    13681 acgtcagtac cgtggccccg ccccgtcgcg cccagcgaac cgtaaagctc gatccgcaca
    13741 ttgaccacct ccggcaacag cccggcctcg tcgagacgcc gggcgaagcg ggcggccgcg
    13801 accatcggac cgaccgtatg cgaactcgac ggcccgattc cgatcttgaa cagatcgaac
    13861 acactcaacg acatgattgt ccccacgctg gcgaatccct tgcgggaatg tgcgtagtac
    13921 atcaaccaat cactgttcaa gctagaaccg ggctgccatt cccctggaac aatcacgcca
    13981 tgcaccccgt gaatcgggaa aaccctgaca caaacagcgt gaatttccag cgatttcgac
    14041 tgcaccgcga cgcgtcagcc cggacgcggc atcatcatgg aaagtgggag cgagcagggg
    14101 ggtaagtacg cctgtgggtg cgggtgttac ggccgcagag gtgcctttgc cacaggcacc
    14161 gctttcacaa gaagaacggc acagcgacga atcaatagaa ttccgtgcga ttcgggaaaa
    14221 tctcgtggta attcacatcg cccaactgaa tctccccttt tttgcgacgg aaaccgctgg
    14281 gagacattgc atagatctgg cggaactcgc gccccatgtg cgaggcatcg gcgaacccgc
    14341 attcggtcga tacgaccgat atgggcttgt cggtattgag caacgaccag gcggccaccc
    14401 gcagccgcag ggcgcgcgcg aacgcttgcg gcgtcttgcc cgtatcggcc ttgaatatgc
    14461 gctcgaactg ccgacccgac acattcaact tgccggccat ctcatccacc gtcaaggcct
    14521 tgctcaggtg ttgctccatg agcagcatgg cacgcttgac caacgggtgg gcggaaggct
    14581 cgagccccgg aggatggggc tgcggcgcat tcaccgtctc ggaccgatcc accagcagaa
    14641 tgcgtagcgc cttctgcacc gtggacacat cgatatgacg cttgagaatc tcggccgcca
    14701 catcgatcga cgcccgcccg cccgaacacg tgatgcggtg gccatccacc acgaacaacc
    14761 ggtcggccac caggcgcgcg ggctccacgt gtggaaactg ctccaggaaa tcccaatagt
    14821 ggaaccagct cacacacacc cggtgcctgg ccagcacccc cgcacgcatc agcgcgaacg
    14881 tacccgtgca gatccccacc agcgtcgtct tcgtttcacc gaccgcgcga atgaaatcca
    14941 gcgtggcgcg gctgggctca gaccccttgc gcagcaaccc cccgaccacc accacgtaat
    15001 cgaaccgcga tggatcaccc agctcttccc acggtgtcgt ctcgataccg cacgacgccc
    15061 tcaccggctt gcggccctcg ccgatcagcg accagcaaca ccgcaccggt cggctattgt
    15121 cgccctcgtc gctcgccagc cgcagcatgt cgaccaaccc ggaaaaagcc gtcaacgtga
    15181 aatccggcag caacaagatg ccgaaccaga ccggccgggt gccggttgtt gccgaaccag
    15241 tatcaggcac gacgcacctc gagaaatcgg gaatcccgca agaatgcact gattttatgc
    15301 gatcctgacc ccgacgcgac tcaagaacca aatcccagaa tccagaacct accccgccac
    15361 tcccttgaac cgattcatca accgcgcaaa cggcgtcacc cggttctgct gcggatcaat
    15421 cccgaaactc tcggtgatcc ggtccaggat gatcgcgacc agcaccaccg acagaccact
    15481 ctccatcccc aggccaatgt ccagccgctg aatactggcc agtacatcat tgcccagacc
    15541 accagccccc accatggacg caatgatcac catggacaac gccatcatga tcgtctggtt
    15601 gatacccgcc atgatcgaag gcatcgcatt gggaatctgc accttgaaca acaactgcat
    15661 cgacttgcaa ccaaacgcct gccccgcctc gacaatctca ttgttcacct ggcgaatccc
    15721 cagcgcggtc aaccgcaccg ccggcggcat cgcgaacacc accgtcgcca aaatccccgg
    15781 cacacgccca agcccgaaga acatcgcagc cggaatcagg tagacaaacg ccggcatcgt
    15841 ctgcatgaaa tccagaatgg gacgcacaat cgtcgcggcc gtcttgttac gcgccgccca
    15901 gatgcctatt ggaattccga acaccagact gatcaatgtc gatgaaaacg tcaggcctag
    15961 cgtcaccacc gtctgcggcc agaaatgcat gttgtcgatc agcagcagag acaacagcgt
    16021 gaacaacgcg aaccgcgcgc caacccgcca caaccccagc accacgcaaa tcgtgatcag
    16081 cagccacacc ggtgtcacct gcaacccgtt ttcaatcaaa cccgaaaacc agtggatcgc
    16141 aaaccccagg gaattgaaaa tatttccatg cttgtccagc atgtaattca caccggcatc
    16201 gacccaatgc cccaggggaa gcttatgact accccaccat gcaaccatat cggcgatctg
    16261 tccgctcatg cgccccccct gttcgcagca atagtcttca gaaccgtcgc gtgacacaag
    16321 gctccacagt atcttccaag ctcgttgacc accggcagcg ggcgatgcga ttgcatcagg
    16381 acatccatca gttcattcaa cggcatgctc gagggaatga ccggcgacga cagcggccgg
    16441 gacgccccca ccttgcagcg ctcgagctcg tcccggttgg cgacgaaccc atgtacctgc
    16501 ttgttctcgt tcaacagaaa cgccccctga tcatcgatgt ccacctgctc ggtcttgtcc
    16561 gacacataag gataggaaat caccgggatt ccgtcaggct cgagaatgtc ggccgctgtc
    16621 agataccggg atgtatccac gccattgaag aacgcacgca cgtagtcgtc ggcgggatga
    16681 ttgatgacct cgcgtggcgt gcccacctgc accaccacgc cgcctttcat gatggcgatg
    16741 cggctgccaa tgcgcagtgc ctcttccagg tcatgcgaca cgaagatgat ggtgcgccgc
    16801 tgctcacgct gcagatcgag cagcacatct tgcatctcgc ggcgcttgag agggtcgagc
    16861 gccgagaacg cctcgtccat caggatgagc gacggatcca cggtaaacgc gcgggccaga
    16921 cccacacgct gctgcatgcc gcccgaaagc tcgaatggac gtttgtgtgc gaatgccgcc
    16981 agacccactt gctccagggt ttccatggca cggcgttcac gctctttctt tgcgactccg
    17041 gccacttcca ggccaaagct ggcattctcg agcactgtgc gatggggcat cagcgcgaaa
    17101 gactggaaca ccatgctcat attgcgtcgg cgcagcgcgg tgagctgggc ccggttcatg
    17161 gtggatacgt tctggccgtc gacgaagaca ttgccggccg tgggctcgat cagccgattg
    17221 atcagacgga tgagcgtcga cttgccagac cccgacaagc ccatgagaac gaatatctcg
    17281 ccctctgaaa ccgagaagga aacattatcg acaccaatga cctgccccgt ctcgcggaat
    17341 atgtcatctt tggtgcgccc cttgcttaga agctcttggg ctaaagcggg gttggcgccg
    17401 aatattttat aaagattttc taccactatt ttatcagtgc tcatattggc ctcgaaaaca
    17461 gaagagttgc cagactgaca aaattcctgc ttttaattag agattgttgc caaaatacgg
    17521 cgattatttg tctaaaagcg acaacatatt gatttaaaag aaaaatcaca tcaaacgcgc
    17581 catgagagaa cggagtttac ccttcaatca ggtcggattt gataggtatt tttcctatgg
    17641 aatccaagtc gcaaaaacta gaacaaccgt cgtgaaagca caagcaaaaa acaggcgtaa
    17701 gcgggatatt tattgaattg ggaatagcta ttcatgagtt ccttttaaaa gcattcgatc
    17761 aaatcgaata tatcaacaag agcgcccgtt tcggacgttt caacaggaga tgcaaaaatg
    17821 gctgacaacc acggcgtggt atatatcgag ccgggaaaag tggaagtcca atccattccg
    17881 ttcccaaaga tgaccgaccc ccaggggcgg aaaatcgagc acggcgtcat tctcaaggtt
    17941 gtctccacca acatctgcgg ttcggaccag cacatggtcc gaggccgcac cacggcaccc
    18001 gccgggctgg ttctgggcca cgaaatcacc ggagaagtca tcgagctggg gcgcgatgtc
    18061 gagcggatca aggtcggaga cctggtgtcg gtgcccttca atgtggcatg cggacgctgc
    18121 cgctgctgcc ttgagcaaaa taccggcgta tgcctgagcg tcaacccggc gcgccctggc
    18181 ggcgcgtacg gttatgtcga catgggtggc tggattggcg gccaggccga atatgtcatg
    18241 gtgccttatg cggacttcaa cctgctgcga ttccccgacc ggcaacaagc aatgagcaag
    18301 atccgcgacc tgacgtgtct gtccgatatt ctccctacag gataccatgg cgccatcatg
    18361 gcgggcgtcg ggcccggcac gacggtgtac gtggcgggcg cggggccggt gggtctggcc
    18421 agtgccgcat cggcgcgact gttgggcgcg gcggtcgtca tcgtggggga cgtcaatccc
    18481 gcacgcctcg aacacgcccg caaggtgggg ttcgaaaccg tggatctgtc gcaggacacg
    18541 ccgctgggcg atcagatcgc cgatattctg ggaacacccg aagtggactg cgccgtcgac
    18601 gccgtgggat tcgaagcccg cggccacggg cacacagggt cgcgcagtga ggcccccgcc
    18661 accgtgttga actcgctgat ggccgtcacg cgcgccgctg gcaagatcgg catccctggc
    18721 ctgtacgtca cggacgaccc gggcgcggcc gatgccgccg cgaagcacgg cagcctgtcc
    18781 gtgcgcttcg gcctgggctg ggccaagtca cacagcttct tcacgggcca gactcccgtg
    18841 ctgaaataca accgcgcact catgcaggct attttgtggg accgcatcaa catcgccgag
    18901 atcgtcggcg tacaggtcat caccctgaat gacgccccca agggttacgc tgaattcgat
    18961 tcgggcgcgc ccaggaagtt cgtcatagac ccacaccaga tgttgaaagc ggcctgatcc
    19021 ccatcagcgc accggcagga agttgaacaa caacaggccc tgcacgtaat tgatgccgat
    19081 acgacgcagg ttttcatcga gcaggctggc caccaggtcc agcctgccta tcagctcgcc
    19141 gaacccccgc tcgaaactca gattactggc gccatcgacc gacatctcgt tcgacaacaa
    19201 caacggtcgc cccgacgcgt cgcgccgcgt ggccagcatc caggcggcgg tctcgacatt
    19261 gcgcgccgcg ttgtacacat gctgcgcatc cagccgatca gtcgcataga aacgtgtcct
    19321 gcccccgtgc gcctcgatga tcatgttggc caggccttcg atgaacgcac gcacgcgatc
    19381 gccccgatac cccggatcca gcgccacgga tagcacctgt atgtcacgca tccccgccaa
    19441 gtcaggcggc acctggcccg tggcgatggc ctggcggcct tgcgccagcg ccgccgccag
    19501 attcgggaaa tgcgacttgt gccattcgac cggattgcgc ttgtagagct tctccatgag
    19561 cgcggacagc gtcgccagat tctgcttcat ggccaccgtc acggtgcggt cgaaatcggt
    19621 ctgacccaat tgatcgaacg acatgccctc gtctttgggg gtaccccgcg tcgtgggcgc
    19681 cgagctgctg gcgcacccgg ccagcgcaag ccccaacacc agcagcgcgc ccaatcggcg
    19741 cgccgccctc gcgccgcgtc ccgcggcgtg agcccaatgg cccgccactt aggccggcag
    19801 cgccgcgatc gtttgtgcca ccgaggcagt cagtttcttg ccgaatggca catgcaggaa
    19861 ttcattgggc ccatgcgcat tggagcgcgg ccccaacacg ccgcagacca tgaactggga
    19921 cgtggggaag cctttctgca gcaagcgcat caggggtatg gtcccgccct ggccgatata
    19981 gccgcagggc gcatcgaagt attgccggga tgccgcatcg agcgcactcg tgagccaggc
    20041 cgaggattcc ggcgcgttcc agccgctggc cacgccttcg tcgggcttga acacgacctt
    20101 ggcgttgtag ggcgcgtcgg cttccagcag ggccttgagt tcctgtgagg ccgcaaccgc
    20161 atccaccagc ggcggcaggc gcagggacag tttgaacgcg ctgcgtgggc gcagcacatt
    20221 gcccgcgctg gccacgggcg gcagcccctc ggccccggtc accgacagcg tggggcgcca
    20281 gtggcggttg agcagcgcct gctcggcatc ggtggtcatg ggcaggacaa agccgccatc
    20341 accgccgcag ctccagggga aacgcttcca gacgtcgtca cccagcaatt tcgccgccag
    20401 ccggatctgg tccaggcgct cggccggcac ctcgcaatgg aagctttgcg gcagcaggcg
    20461 gcccgtggcg ctgtcctcga ggcgatccag caggtgacgc agaatgcgga agctggacgg
    20521 caccacgccg ctcgcgtcgc cagagtgcac gccttcgtcg agcacttgca cttccagtgt
    20581 gcccgccacc aggccgcgca acgatgtcgt catccacagc tgatcgtaat tgcctgcgcc
    20641 agaatccagg cacaccacca gcgcgacatt gcccagacgc ggacgcaacg cgtcgacata
    20701 gggcagaagg tcgtagctgc ctgattcctc acaggtttcg atgatgccca cgcagcgcgg
    20761 ccggcccacc ccctgcttgt cgagcgcggc gatggccgtc agcgaggcgt agacggcata
    20821 gccgtcgtcg gcgcccccgc ggccatacaa cttgccgtct tcgtacttgg gggtccaggg
    20881 cccgagatcg ttgcgccacc ccatgaattc tggctgcttg tccagatggc cgtagaacag
    20941 aaccgtatca ccactgtcgc tgcgcgtgga cggtgcgtcg aaaaaaatga ccggcgtgcg
    21001 acctgccagc cggaccacct cgagcttcag gcccgacacc tgctggtcct cgacccactt
    21061 ggccgcgttg cgcaccacgc gctccaggtg ctcattggcc acccattcgg ggtcgaacgc
    21121 cggactcttg gccgggatgg cgatgtagtc ggtcagttca ggaacgatgc ccgtatccca
    21181 tttttcgtcc acgaacgtct ggagctgatc gggattcaac gccaaagaag cggtgtcgtc
    21241 ggggatgcgt gcgttcatgg gggcctcgcc ggaatggatt gaccgatcga ttgtacgctg
    21301 gtcccggggc gggtcggcaa cggtttatga tgacgggcgt ccgtacggtg ccctgggaat
    21361 tctcgcggat cgcaggtcct gataccgcgg gcccagatgc ccgggccggt tttcccgagc
    21421 gcaacgcacg ccccccatca acgcagcaag aaggacttgc catacatgtc agatctttcc
    21481 gccttcccca tcacgaagaa gtggcccgcg cgacatcccg accgcatcca gttgtattcg
    21541 ctgcccacgc ccaacggcgt caaggtgtcg atcatgctgg aagaaaccgg cttgccttac
    21601 gaaccgcacc tggtcagctt tcagaccaac gaccagacct ccccggaatt cctgtcgctc
    21661 aaccccaaca acaagatccc ggccatcatc gacccggcgg ggccgggtgg caaacccctg
    21721 ccgctgttcg agtccggcgc catcctcatc tacctggccg acaaatcggg gcggttcctg
    21781 cccacggatg cggcggcccg ctaccaagcc atccaatggt tgatgttcca gatgggcggc
    21841 atcggcccca tgttcggcca ggtcggcttc ttcaacaaat tcgccggcaa aagcttcgag
    21901 gacaagcgcc cgcgcgaccg ctacgtagcc gaatcgcggc gcttgctggg cgtgctggat
    21961 cggcaggttg cgagccaaga ctggatcatg ggccgcgact acaccatcgt ggacatcgcc
    22021 acctggccct gggtgcgcaa cctgatcggt ttctacgaag cgggcgacct ggtcggcatc
    22081 ggcgacttcc cccaggtgac ccgggtgctg gacacgttcc tggcgcgccc ggcggtgcag
    22141 cgtggcctga acattcccgc gcgcgattga cgcggcccgc aggtcgcccg acggggccga
    22201 tcatggccac gaagcgacga tcgatatcat tggcccacga tgactcatac tgcccagtca
    22261 cacccgctca ccttccgtcg cctgctggct ccggacgctc ccgccttcca gcagcttcgc
    22321 ctggaaagcc tgcacgatca ccccgaagcg ttttcggcca catgggaaga agaacgcctc
    22381 cagcccctcg agtggtttgc gaaacgattg gaaaatagtg tcgttctggg cgcctggcgc
    22441 ggcgacacgc tggtcggcat tgccggactg ttcgttccac tttcgccccg gcagcgccac
    22501 aagaacgcgc tctggggctt ttatgtccaa ccccaggcgc gcggggccgg gttggcgcaa
    22561 gacctgctta gccggatcat cggccaggcc gccacccgtg ccgaagaaat cctgctcggt
    22621 gtcgccgcct caaacagccc tgcactgcga ctctacaaga aggccggctt ccaggaatac
    22681 gggctggaac gccgggcgct caagatgggc gaccactacg acgacgtcat cttgatgtcg
    22741 ctggcgctcc cctctgcgcg accaacgtga tcttgccggc ccctcggaca gggaattaat
    22801 ctcatccaca gtcagcgcaa ccccagaatc gccagcgtct tttcgtcggg ctcgccgccg
    22861 cagtatttgt ccagcgcgtc ggcgaacacc tggacatcct cgccagcctg cgcgaacagc
    22921 gtcatgcagg agcggaattt caagtcgtcg ggatacgcca tgacctgccc cagtgaacac
    22981 ccctcgatcg cattgaccag ccgggtgcac cggcgcagcc gctcgcccag caccgggtgg
    23041 cgcagatagg cgcgggcctc atccagtgaa gagatcgcaa aacgccgggc catcggacta
    23101 cctcccagcc cctgaatctg gggaaaaacg aaccacatcc aatggctttg cttgcggccg
    23161 gcccgcagtt cctggaatac ctgctcgatg accggttcct gtgcgttcac gaaacgctgc
    23221 agatcatgcg gatcgtccat ggccacctcc atgccggcaa acccggcgac gcgttggcgt
    23281 ttcccggtac ataatgtaga tgatttctct cgcgcgccag ggccttcccg gccccgggac
    23341 agcacccaac ccgccaccca accccgatcc cctgatgaac accaccattc tcccctctta
    23401 cggcgacgtc gtcgcggccg ccggccgtct ggacggccag gccaaccaca cccccgtgat
    23461 gacctcgcgg gcgctcgacg aagaaaccgg cgtccggttc tacttcaagt gcgaaaacct
    23521 gcagcgcatg ggcgccttca agttccgcgg cgcgttcaat gcgctgtcgc ggctatcgcc
    23581 cgaacaacga aaagccggcg tcatcgcctt ctcgtcaggc aatcacgccc aggcggtcgc
    23641 actggccgcc cggatccttg gcatgcccgc caccatcgtc atgccgcagg acgcacccgc
    23701 cgcgaaaatg gcggccacca agggctatgg cggcaaagtc gtgctgtatg accgctacaa
    23761 ggaagaccgc gagcagatcg cccgcgacct ggccgacaag cagggcctca cgttgattcc
    23821 accttacgat catccggacg tcatcgccgg ccagggcacc gcggccaagg agctgctgga
    23881 agaggcggga gagctcaacg ctctgttcac ccccttgggc ggcggcggcc tgctggccgg
    23941 gtccgcgctg tccgcccggg cgctggcacc gaattgcagc gtctacggcg tggagcccga
    24001 ggccggcaac gacggccaac aatcgttccg ctccggcgcc atcgtcaaca tcgacccgcc
    24061 ccgcactatc gccgacggcg cgcagaccca gcaccttggc ggctacacgt tccccatcat
    24121 ccgccgggac gtggccgacg ttctcaccgc caccgacacg caactcgtgg attgcatgcg
    24181 cttcttcgcc tcgcgcatga agatcctggt cgaacccacc ggttgcctgg gtttcgccgc
    24241 cgcgcggcac atggcgccgc agctcaaagg ccagcgcatc ggcgtcatac tcagcggcgg
    24301 caacgtcgac ctcgaacgct tcagcgagct gctgcgggca tgaccctggc cgcgcgccgc
    24361 agcgcctgag gcgggtggcc gaaaagccgc agaaacgtcc ggcgcatgcg ctcggtatct
    24421 gaaaaaccga cgccccgcgc aacttgctcg agcggctccc ggctttcttc gatacgcgag
    24481 cgggcggcct cggcgcgcag gcgctcgatg gccttggccg gcgtgacgcc tgtttcagcg
    24541 acgaacgtcc gggtgaactg ccgcgggctc aagcaagcca cctcggccag gcgttcgacc
    24601 ggcagggcct cgtgcaaatg ttcacgcgca tacgccaacg cctgtcgcag acggtcggaa
    24661 ggcgggtcca gcgcctgcag ggtggaaaac tgcgactgcc cgccaggtcg gcggtagtac
    24721 acgaccaggt cgcgcgccac agcgcgcgcg acctgtgccc ccatatcgtc ctcgatgagc
    24781 gccagcgcca ggtcgatgcc agccgtgatg ccggctgacg accacaccgc gccatcacgc
    24841 acgaaaatgc ggtcggcctc cacacggacg cgcgggtagt cccgctgcaa gcgggccgca
    24901 tggcgccagt gcgtggtggc acggcgcccg tccaggacgc cggcgcgcgc cagcagaaac
    24961 gcccccgtac agacgcttgc cagccgccgc gacgcggccg cctggacgaa cgacaccagg
    25021 gagggcgact ccagcgccag cgaaaccccg cgaccaccca ccacgatgac ggtatcgggc
    25081 gcataggcac ccagcgggct ggtgacgacc tccacgcccg agctgcttgc caccagcccg
    25141 ccggcctcgg acagcacccg caggctgtag acgggttcct ccgacgtccg cgcggccgtg
    25201 tcgaacgccc ccagcggccc cgaaagatcc aggagattga aacccggata caccacaaag
    25261 ccgatactca acgccatgtg cgcctcctgg cccagggtac gcgccccacg agcgatggtg
    25321 tctttaaaag agggttttat gtcatttacg ccattatcgt accgcatgat aatgaatcca
    25381 tccatacccg gcggcaaccc acccgcacta ttccaacctt ccgcgccccc cctaaagagg
    25441 taacccatgg ccgactcgct caatatcgtc ttccccattt accccggcgt cacccacctc
    25501 gatttcaccg gaccgcacca agtgctggcc aggcttccag ggacccggat caccgtcgcc
    25561 tcggcgggtg gccagcctgt cgccgctgac gggttcaact tcaacgggct ggcccggctg
    25621 gaagatgtgg atcattgcga tgtcctgtgc gtgccgggcg gccacggctg catcgccgtc
    25681 ttacaggacc cggtgtacat ggcggccata cggcggctcg ccacctcggc gcgctacgtc
    25741 acgtcggtct gcaccggttc gctgatcctg ggcgccgccg ggctgctcgt cggcaagcgc
    25801 gccgcctgcc actgggcctg gcgcgacctg ctcgccccgc tgggtgccat acccgatccg
    25861 gcccgcgtcg tgcgcgacgg gaacatcctg accggcggcg gcgtcaccgc cggcatcgat
    25921 ttcgcgttca cgctggccgc ggaaatcgcc ggcgaagagg ctgcgcagac gctgcaactg
    25981 ggcctggaat acgcgcccgc gccgccgttc aattcaggca gcccggactc agcgccccgc
    26041 gccgttctcg aacgggtcca ggccctcaac gccccgcaac aggccgcgcg gcgagctgcc
    26101 atcgaccagg ccgcccgcgc gctggggtga tgcctccgca cgggcagcgc gagagcgcgg
    26161 cgccccatgg cgacagggct cctgacgctt ctggaacact cacgtcgcga accagattca
    26221 aaatccaaaa aggtagtgtc tgtactggta tgaaaaacca tcttgtacca ggataaccac
    26281 cggtatattg tgggcattct tctttccgcc cccaaggatg caccatgaca caagcacacc
    26341 gcgacacggt ccactcgcaa ttcgaccccc aggcgaacgc ctacctgcaa agcgccgtgc
    26401 atgccgccgg ccccgacctg gagcgcgcgc gcgaaatcgt cggccaggcg gtttccgccg
    26461 caagcgccac cttgctggac gtcggctgtg gcgcgggcca cctcagcttc gccctggccc
    26521 cggccgtgct tggcgtcgcg gcggtcgacc cgtctcccgg catgctggcg accgtggcgc
    26581 aggccgcggt cgaccgcggg ctggccaacg tcaccacgtg ccaggccagc gccgagcaac
    26641 tgccattcga cgacggcaca ttcgacatcg tcgccacgcg ctacagcgcc caccactggc
    26701 tggacctgcc ccgcgccttg aaagaaatgc gccgggtggc caaacccacg ggccgcgtcc
    26761 tgatcattga cctggagggc gacgaaaacg cgctggtcga cacgcacctg caggccatcg
    26821 aactgctgcg tgacttgtct cacatccggg atcgttcgcc gtcggaatgg gggcggctgc
    26881 ttgcggacgc gggctttacc ggtgtcggac acgaatcctg gcccacccgg ctgatgttcg
    26941 aaccctgggt cacccgtatg cgcaccgcac cggaacgcgt cgcgatgatt cgcgccattc
    27001 agcaggaatg cccgcaagag gtgcgggcag cgctttccat cgaggaagat gggtcgttca
    27061 ccgcccgcac ggggctctgg tggggcagcg ccgcctgaaa gctgaaaacg cggcagcacg
    27121 cgacggcggc cgtttaccga agccgccgat catcgaccgc gcatcaggtc gatgaggcgc
    27181 cgatcgcgct tggtcggcct gcccgcggcg acatcgcggg cgggctcagg cgccaggcgc
    27241 ttcatttccg ccgcccgctc ccgagccgcg atgctctcag gcgtttcctc ataaagcaga
    27301 ctcgccctgg gtgccggccc gcgcacggtg ctgagggctc gcacccatac gtgaacggtc
    27361 gagccctcct tgcgcacgct gacctggtcc cccggcgcaa tctcacgcgc gggcttgacc
    27421 gcctggccgt cgacctgcac ccggcccgcg ccgatctcgt gcgcggccaa gctgcgcgtc
    27481 ttgtagaagc gcgcggccca tagccatttg tcgagtcgga tcctatccat gatgcatcgt
    27541 cgttgcggga ccgccccgcc acatctattt tatgtgatcg gcgccacgtc ccggtcaacc
    27601 cggccgccgc accacacgaa cttttccgct ggtcccgcca ccacaataga aaccgccagc
    27661 cccgtcgggc tcgagcccgc tcacgccgat tccgcacggc atctcgaggg actcgagcac
    27721 cgtcccgctg cgtgggtcga tgtgctggat cccgccctgt tcgtcttccc acgtcccgtg
    27781 ccacaactcc ccatcgaccc aggccacacc cgtgacaaag cgattcgttt caatggagcg
    27841 caggatgtcg cccgtggcgg ggtcgacccg atgaatcttg cggtcgcgat actggccgac
    27901 ccacaggctg ccctcggccc aggcgaggcc cgagacggca ccgtgcgccg gagccggaat
    27961 cgatccaatc acttggccgt ttgatggatc tatcttgtct atcctgtccc cagcgatctg
    28021 ataaagatgg gtgccgtcga atgccgtgcc ggcatcacag gcgcagtcca gcgtgcgctc
    28081 gatggtgccg ctgccgggat cgatggcgac cagcctggca cctgtggcgg cccagacgcg
    28141 ctggccgtcg gaggtcacgc catgcacggc gtccaccccc tcgaacgggc catactcgcg
    28201 cacgatattg gcggctttcg ccgttgcacg gggcttgagc gaagtggtgg cttgggtcat
    28261 ggctgtctcc tggttgcgcg ccgcaagccg gcgccttcag accactttat cccaccggca
    28321 gcgcggcggg gagtaacaag atcgtcgtga atccggccag cggcggcgac agccagcgct
    28381 gcgcccgcgc ccgcccaacg gatcgcaccc gcccgaccga ctcaaggtcg accaacactc
    28441 gctggatggt gcgctggctg gcccccagcg ccagtgacaa ggccgacgtc gaccaggcgg
    28501 caccgtcgga cagcagcgcc atcagggcgg cctgttcatc atcctcgaag gggggcgcca
    28561 gcaccgcgac ggcacgcccg tccatgggtc ggatggcaaa accaccgctc gtggcgtcga
    28621 tccgcgccaa gtccttcaac accgaacgca atcgactcat ttcgacacgc agccgtaccc
    28681 tgtgggattc atccggccgg cgcatgcgaa acacctcgtg tatcaaggcg tcccgctcga
    28741 tgtcgcccgg ccacacccgg gccagcgccc gaaccagcgc gaagagtatc ggacgacgcg
    28801 tcagaaagca ccaggccgtg ccggtgccaa cccgccggcg gcaagcatca accaccagcg
    28861 tgcccgactc cagcagctca gcgacctgcc ccaatagcaa agcctgttca ccttcgtgcc
    28921 cgacccggcg ggcggcgggc tgctccagcg ccgcgccggc ctgcgccacc tcggccagaa
    28981 gggccgccac gccggacttc cgcgccgcat tccgcgcgcg aaccaatgcg gctttcccgg
    29041 cctccacacg cagcgcccgc agggccagct ccgcagcggt cagttcagca gccgccacca
    29101 gcgacggtgg ctgatcacgc atgtcgagcc cgctcaaggc cgtctcggct tcgcgcagac
    29161 gccccgtcag cacttgccaa cgcacggcga tgagccgcgc atgcatcgcg ttgacagggt
    29221 cggcatgctc ttccagcgtg gcgatcgccg ccgccaacga gcgcagcgaa ccacccaggt
    29281 tccgcgccgc cagcgccacc tccgcctcgg ccacggcaca acgcgccctt gaaagcacct
    29341 cgtgagcacc gaatccgcgc cgcgcacgcg acaacaattc acgggcgcgg acatgctcgc
    29401 ccagctgcgc catggcaatg ccgcgcagcg ccagcgcggg cggatcgtca cgcagggcga
    29461 tacgcttcag ggctcccaac gggtcgccat tcatcagggc gcgggcggcg gcggtgatca
    29521 gggcatccat cggggaagaa tacaccgatg cccgcccccc gcactccgcc ggcgggatgg
    29581 gatagacccc agggattccc tgaacaaatc accgcgccgc atgcctccgc agcgcgactt
    29641 gctcagagga accttgcgcc gctcctagtg atcgccttcc cgccgttccg gacgcccgcc
    29701 accccgcctg tcctgatgcg gacgctcggc gttattctgc cgacgctgtt gggattcgtt
    29761 ctgccgcgga ggccgctgcg gaggcgtatt gcgatattca ttccggcgct gtgtcacggc
    29821 ccgctcctgt gaaacgggtg gccgaggctg gttcacacgc ggtgattgtt ccggccgggc
    29881 cggccgctgt tcgatgcgac gctcttgctg aagccgctgt tccggacggc ccgcttgttg
    29941 cggttggacg cggccggcct gccgcggttg cccgggattg ggccgcgact cgggatgcgc
    30001 catgtactgg cgcgccggcg cctccggccg cccccgattc atgtcgaaac gctgcccccg
    30061 gtcacgttcc gacatctcgc gctgccgtgc ctggaacgac gtgggcgcgt aatgatgctg
    30121 gttcccgtag gaacgctgcg cggccgtcgg cctcatcacg ataccgccgc gcccaccgtt
    30181 gtagctcgtc cggttgatgt ggacgttgtt gatcaccgtc ttgttcacat acacgttccg
    30241 gatgttggtg acgttgatgt ggttgactgc gcggttgtag tagaaatcgc gaccgcgcca
    30301 gtagccaccc acatagcccg tgccgaaata gccgaaccca taattgatcc caccatagaa
    30361 ccccacgcgc ggcccccagt atccaggatg ccagcggtag aatccatccg accaccccca
    30421 ccaacccggc gtccacagca ccccgacgat gggcggcagc acccacaccc ccggcaccca
    30481 atagtagtag cccaggttgg accaggccca gtaacccggc gtccagatgt agcccggtcc
    30541 cggtatggcc ggctgttcgt acacgggtag tgcgggaggg gaaacgttga ccgagatacc
    30601 cacgctgaca gcggcggcgg cgggcagcgg cagcagcgct accggaatag tgaatagact
    30661 tgcggcaagt acggcggcag agacgcggct catgacgaca cctcgaaata gcccggattg
    30721 gcgagcaaag tataggcttg gggttgaaac aaaagtacta cagaaaccgc caatcccatt
    30781 acgccacgtt acggggttcc ggcgatgatc gccatcacgg atattcggat atttcgatca
    30841 gattgagatc gggatcccgc acttgcgcct tgggttcgag ctcggcgcca cgcacgtgca
    30901 agctgaccac cttctggttg ccaaagcgga atgcaatccg acccgcgccc cgaatcgttc
    30961 atcattggtg agcattgttt cgacttttgg ggcattcaca tttcaccccg aaagccgtag
    31021 tctatcgaga cacatccagg gaggccctcc atgcgactcg cacgtcccat tctcatcctg
    31081 ctggccatgc tcatcgcatc gccggccctg gccaaaatga tcgcccaacc ggtcagttgg
    31141 acgctggacg gcacacactt caatagtgtg cttgtctatg acaacgcctc acaagccagg
    31201 cgccccggcc tcgtcatggt gcccaactgg tccggcatca acgacattgc cgtggaaaag
    31261 gccaaacgca tcgctggtaa agactacgtc atcctgctga ccgacatgta cggaggcggc
    31321 atacacccca agaacacgga ggaagcccag gccgccgtcc aacccctgta tgccgaccgg
    31381 aatctcatgc gcgcccgcat tcgcaaggct ttctccgagc tcaaggcaca agcccgccaa
    31441 gcgccaatcg atgtatcgcg cctcgctgcc atcggcttct gcttcggcgg cgccgccgtg
    31501 ctcgacctcg cccgcagcgg ctcagacgtg aaggccgtca tcgccttcca tggcgacctg
    31561 cgcaccgaca atcccgcgct cgcgaagaac atcaaggcac gcgtgctggc catcaacggc
    31621 gccgacgaca ccagcaccat gccggccgtg ccggcgttca ccgaggaaat gcgccaaagc
    31681 cccgccgact ggcagttcgt ggaaatcggc catgtcgtgc attgcttcac cgagaccgag
    31741 gcaacggcca agaccgggaa ttgccggtac gacgcgaagg ccgccgcgcg gtcgtaccgc
    31801 atgatgcgca attggctgag ggacagtttc aaggagggat agcaggacgt agacccaccc
    31861 gcgcatcgga gccatctggt ttcgaattcc ccgcttgcca cgatcagcac cacggcacgc
    31921 tggtgcgggt gccacctggg taccacacca accagtacaa cgtgtacacg cgccatcctt
    31981 cgcgtcaatg tcagcacagc gccgagacat tgattgcgct tgccccgcaa gcccgcacca
    32041 gttcacgatc atggctggcg aacaacacga cgcgatcccg ggaccacacc ttgaactgct
    32101 cggccaacac ggcgcgggcc cgcgcgtcca gcccgtcgct ggggccgtcc gcgatgacag
    32161 cgaccggatc acccaaagcg gcggccgtca ggaagacctt gcggcgcgtg cccgtcgaca
    32221 tctgttcgaa acgcttgtcc agatgcggct ccagccctag ttcgtatgcg aaatcaagca
    32281 cggcatcgcc gagcgtcgtg ttcttttccg tagcaacctg ctcgagcaag ccacgccccg
    32341 tctgcaaggg aaacgccaag caattctcag gcacataagc caggcgggcc ttggcctgct
    32401 ttggttcgcg gaccaacgaa tacccgtcga tccagacgtc tcccgcatcc ggcacaagaa
    32461 cgcccgcgat gacgcccagc aagctggatt tgccagtgct gtcctcttcg cacagcgcca
    32521 cgcatcccgg cgggaacgtg cgggtcagcc cctggaatac aagatggtcg ccataacgct
    32581 tgctgagatt ttcgatgcgt atcatgtccg gcagtgtact ttggagagag gtgattcaga
    32641 ggattcctac cgcttgcgcg gcacgcccaa taactctgac cgcccaatca ctgttcgcgc
    32701 gctttctggc cgcttcggct cagaaaaccc cgcgcgggca atcgcgtcac ccaacccctg
    32761 gaacgcgacc gagcctttcg agtcatgcag cgaagccatc ccctcgttcc acgcatgcac
    32821 catgtgcttg gccacggtat gccagcgcgg ctcattcctg gctgcctcga tcacttccag
    32881 gccgacatcc acggcggact cacataatcg ctcaaccatc tcggcatatt gcttcggcgc
    32941 gatgcccaat cttgccttga agaaccgctc cagcgtcttg ccaaccctcc aagtctgccg
    33001 cccatccaca ggtaaccccg gtgggttggc ggcatagcgc ggataggcct gtgtcgtcac
    33061 aacatcgtag accggcgtat aggccacatc tttcaggctg gtgtagtaca aggcgatatt
    33121 cttggcatgg caatccgcgt tgcgcaccac ataatccgtg agcaaatgcc agccgaattg
    33181 ctccaattgc gaccgcacgt ttttggccgg cacgtatacc cgggtcgctt tcaggacgcg
    33241 ctcgatcgtc gtcgcgtact tctcgtgcgg gggaagcccc agcaaaccgc aggcgtcttc
    33301 gacaccatgc gtcggctcgc catgttcatc cacatcgaaa cgatccacga ccagcacacg
    33361 cccatcttcc gacatgcgca tcccagccac gggtacgaca ttcaaccgtt ccagcacccg
    33421 catggtgtgg aactcgttga aacccaggta gggcgtgccg tcgtccgaac ctttgattat
    33481 gtggcggctg gttcgcaatg tcggcttgcc caaaggatga tcacgcttcc ccggcccatc
    33541 ttccggcgca atgaacttgg gcaccatacc ggagatggat gcgcgggcat accggcgcac
    33601 cagaccggcg aaatgctctg tggtgttttc cgccttcagc aaccgctcga tctgcagcgg
    33661 ctccctatcc gcgtcaagcg cgccgccttc aggcgtgacg gtgacacgcc ctatgcccat
    33721 gccacccacg accgccagca acgacagatc tgtgccatcg agcagtggcc caaactcctc
    33781 acggatgacg ctcagcaaat agccttctgg caggttctgg cgaaagaaag gatgcagttc
    33841 gcgcggccag cgccagggct cttcacgcac cggcatggcc aggctgacaa agtcagccgg
    33901 atcggtgcct gggagatacc tcaggacgta ctcatcgcgt tcccgataga gactcgcgac
    33961 cgtccgcccg cagacacgta catcgagttt catggctcgt gccccaatcg ctgctcggca
    34021 agaatgtcct cgatggtacg gacatggccc tgcttgatga gtttgatgtc gtagcctgcg
    34081 gcctccagta aacgcaccaa tgcggaaaca ctcatgtccc ccttggccag tgtttccatg
    34141 cgagcgacgg tcgtacgcga gacgcccgcg cgctgggcaa gcgtttcttg gctgaggcca
    34201 gcctcctggc gagccctgcg cagcatgttg gccacgtcgg ctaatgtgct cactgtatct
    34261 cccgggcatc aaaattggac taacatgaaa taaatgtagc cccagagata caaataaaca
    34321 agaaaaagct gagcgctcga tgatgatcca aagtgaggat cagcgtaata ggccattttg
    34381 tagcccaaga gcatcgaata ccaagatagc gttcaatttt gtagcccaga aggcacatgc
    34441 caggaaacag agaacgcccc atacatccgc tcgcctgccc gaccccatcc aatccctcac
    34501 tgccgatcag caagcagcgc ctcgatcaaa gcgtacgtcg ccttgacccg ggcctcgttc
    34561 gggtaattcc ggttcgccag cacgacaatc cccaatcgct cacccggcaa caccgccaca
    34621 taccccccga atccattcgt cgacccggtc ttgtccagca agacattcct ctgtggcggc
    34681 aacgccggag ttatcttctc aaccggctgg ggatgcagga tgaagtcgta cccattcccc
    34741 aacaacaatg ttttcaggct gaccggccgc gggtactgtt cccagatcat gtcctgcgtg
    34801 aagtaggccg tcttgaaccg cccctcttga gtacgatgaa tggcggcttt caattccggc
    34861 gataggaccc cgttgccaag ttccgcgtcc agatacttga ccatgtcgcg ggcgctggac
    34921 ttcacaccat aagcctcggc atccagcacg cctggggtca cgcgaaccgc cttgtcggtt
    34981 tttcgatcgt agccataggc ataatttttc atggcactcg ccgggacgtt gatccacgtg
    35041 tgcgtcaaac ccagttcggg aaacaacatc gtctccgccg cgcgcgcgta aggcatgcca
    35101 agggcctgcg cggtgatgta cccgagcaga ccgatgctga tgttcgaata gctgcgcgct
    35161 cccggctgcg gcgggcgcca ggttttcagc caagtcacca gttcaccgac gctcttcacg
    35221 tcatccggaa cctgcagggg aaggccaccg gaatgatggg tcgccaggtc catcaaggtc
    35281 atgtcgtcgc caatcctgca ggcatcggcg cacaggtagt gggcggcttt gtcgttcagg
    35341 gacagtttgc cgcactgctc cgccagcgcc gccaatgttg cggtgaagac tttgctgata
    35401 gaccccagtt cgaaaagcgt atcgggcgtg acgggccggt tgtccgcccg cgaggcaagc
    35461 ccggttgagt agaagtagtg cttgccgtga tacgtcacgc caacgacaag cccgggtatt
    35521 ttgtacgttt tgatcgcact ggcaaatgcc tgccgcgcca attcttgaaa tggcaacgcc
    35581 ttcgcatgag cactctgcaa ggaaagcgca agaaaggtca gccccagtgc gagtgattta
    35641 agaaccgtca tttcactttt ccgtgatggg cgtgggcacc tgaagccgga cacccaaagc
    35701 gcataatcat tcctaccgct caatccgcca tagtatgaga atcgccatgg gcgcgacatc
    35761 catccttgca ctacatcttg tggtcatcgc cttcaacgtc gcgggctgca tcctcatacc
    35821 cgtcggggcg ctgcttagct ggcgctgggt gcgcggattc tggtggcgtg ctgcgcacct
    35881 cgcgagcctc gccgtcgttg ctgcgcaggc gctgttaggg cgggcttgct tcctgacgat
    35941 ctggcaggcg gaccttgcga atcaccgcca cgccgcgcga ccgctcatcg cgagctggat
    36001 cgatcggttg atctatctgc cgttaccgat ttgggtgttt gcgacggcat acgtcgcgat
    36061 tttcatctat gtcgtggcgc tttgggtgtg ggtgcgcccg cgccgcaggc gcgcgtgaga
    36121 ttcaccgtcg tggccacagg ccacagtatt tctggctgcc ctccaggttc gggccggtgc
    36181 tgccatcagc ccggaccact ttgagccagc cgttcggatc ccgcctcgtg ggtcgatccc
    36241 cgcaattatt gtggacccag tggatcccgc ccctgaatgg aatgccgcag gcttcgactt
    36301 tgtcgcccac cacgatggtg ttcaacggtt ccggcacttt cctggagtgc ggccgatagc
    36361 ccgacgcgaa gacattgtct atggcgacat cgtaggtcgt gccatcgttg ccctgaagct
    36421 tcaggtgtgt atgcgacagc tcaacgccca tcttgcgcct gccgcgcttg aatttgggcg
    36481 cggacaccac ctggccaacg agcaaggtac cgccggctgc ccggcactcc cgcgcatcgt
    36541 tggactgtgc gctggcgccc tgggacgcga ccgccagcaa gacgccgata aggatatggg
    36601 ccggtatcca ggcgagacgg ctcgcgcgcg ggttggggtt gataagcggc atctattttc
    36661 tccattcgtt gagagcgggc cagtgccaat tcaaccatga atctggcgga ttgagtgcgc
    36721 agacaaggcg atcctggcgc tcgaaacgcc gacgtcgcat aacggaacag ccgcccccaa
    36781 ccctttttcc cctatctccc ggtgtcgttt ggcaagggct gctactccgg aatatccttc
    36841 agatccggat catccacttt cacatacgcc agtcccgtca tgttcgcgaa caggtgcttg
    36901 ttcgacattc ccgaggcgac gtggacgact cccttcggat ctttgtagac gaacccgggc
    36961 gttccggtga gccccagatc cttgaatagc ttcttgttgc gatccagcgt cttctggatg
    37021 ttggcggtga cgagactctc attgaaagcg cccccaccgt cccgcagccc cttccatccg
    37081 ccctctccct cggcaagcgc cttggaaggg tcggcgctga gcaggaccca ggcagccttg
    37141 ggagcgctgt ccgtcatgag gaacgcgatg ggaatccagc gcacctgcag gccttcggcg
    37201 tagtaatgcc gtgaaagcct ccagctcaga tggcagaaga tgcaattggg gtcgaagaac
    37261 gcgtagacgg tcgccttctt tgtggcgtca ctggcgccct ggatgatgta cttcgactgg
    37321 atgacctgct gccagagcgc ctggagcttc ggattagctg cggtggcggt ggcggtggct
    37381 tgtgacgccg cagcggccgg agccggtgca gcctgggcaa gacccgccgt ggtggccgcg
    37441 gcggcgagcc atagcgccat cgcggataac cggcgcatgg gtgaagcgta ctgcatgaaa
    37501 gcctcctgga aggctggaaa tcggggttcc tgaagccatc attctagcca cttcggactt
    37561 gaggctcacc cgggagcctc cgggcttgtg gtggtaacac cctttgatcg gcaatccgat
    37621 cgaccgatac gcgcgataga atttgtccct ctgacctgcc cggacgccgg cgtccgttga
    37681 ttacctgtta agcgtctaaa tggaggcaac aatgacacga gttcgcgtcg cgggcttcac
    37741 catctcactc gacggatacg gagcaggtcc gaatcaagac atcaacaatc cgctcggcat
    37801 tggcgggacg gaactgcacc agtggctagt cccaacacac acattccagc gtgccttgtt
    37861 cggcaaggac ggcggcaaga ccggggtaga cgatgacttc gccgcccgtg gcttccagaa
    37921 tgttggggcc tggattcttg ggaggaacat gttcgggccc attcgcgggg gctggccgga
    37981 cacaaactgg aaaggctggt ggggagataa tccaccctat cacgttcccg tcttcatctt
    38041 gacccatcac gctcgtcccc ccatcgagat ggaaggcgac acgacgttcc atttcataac
    38101 cggaggcatt cgcgaggcac tcgaccgtgc acgcgaagcc gctggcggaa tggacgtgcg
    38161 gatcggtggt gggtcgggca caatccggca gtatcttcgt gagggcctca ttgatgagct
    38221 gcacattgcc atcgcgccgg tcctgctcgg tgggggagaa gcgctgttcg aagggattga
    38281 cttgcgggct ctgggatacg aatgcgttga attcgtagcg tcggggaagg ccacgcatgt
    38341 cgtcctgcgg cgccaggggc atacggaggc ccgacccgct ccccaacatc cgaaaccagc
    38401 atctgcccaa ctatgacgta agtcgcccat tggcaacttc gttacgggaa aagagcgaac
    38461 cgggatcaat agtgataacg gcaagaaatg atcgtgatgg tggcagtatc caccgcgtac
    38521 acaaggcgat tggtatcatc catgcgccgt gaccagaagc ccgacaggtt ttccttcaag
    38581 gcttcgggtt tgccgatgcc ctcgaaaggc gagcgccgca catcgtcaat aagcttattg
    38641 atccgtttta acgtcttgcg atcctgacct tgccagtaaa cataatcgtc ccacgctgca
    38701 tcagtccaac acagcaagcg gtcagccatt gatcagttcc tgcggctttg ccttaccagc
    38761 gcgatattgc ttgatggaac ggctcagatg ctccacgttg gcaggagagc gtaacagatg
    38821 cactgtttcc atcaggctgt tgtagtaatc cagcgacata accacagcgt ctgcggtatc
    38881 ccggcgcgta atgatcgtag tatccgcatc gttgacaacg gcatccagta ccgttttcag
    38941 gctgttccgc gcctccgaaa aagagacaac tttcatggca acctcacttg ttcaatttat
    39001 tgtacaagtc taccaaaaaa accgaaaccg acgccacgaa acagacaagt ttgtcatgcg
    39061 gtcaagatcg tgtagggcaa cgacaacatc aaacatcaat attccccgcc tgcaacgcat
    39121 gcgcctcgat gaacgcccgg cgcggctcaa cgttatcccc catcagggtc gtgaacacct
    39181 catcggccgc gatcgcgtcc tcgatctgca ccctcagcaa ccgccgcgcc gtcgggtcca
    39241 tggtggtttc ccacagctgg tccgggttca tttcgcccag gcctttgtaa cgctgcttgc
    39301 tcactccgcg ttcggcttcg ttgtgcaacc atttcatcac ctcgcggaag tcggcgacgt
    39361 atttttcctt gcgcttgtct cccgtgccgc gcacgatcag cgcgccgggg ccgatgaggc
    39421 ccaggaaggt cttggccgcg cgggccaggg tggcgtagtc ggcgccgcgc acgaacgggc
    39481 ggtcgaacac gctgacgcgc gtgttgccgt ggtgcaggcg cttgaccacc acacgccagg
    39541 cgggaatggg ctcgcccagt tcgttggtgg ccgtggtcgc ggccggctcg ctggtcacga
    39601 tgatggaccc cggcaacgcg gggtcgtcca gcacctcttg caggcgctcg gccgacaagc
    39661 gggcgttctc ttcggtgtcg agacggatct ccaccccctc ggccatggcc gacagcgctt
    39721 tcagatcgag cgtgcgtgac aggcggttga tgacggaatc ggccaatatg tattgacggg
    39781 ccagttcggc cagggcgtcg ccggtgatgg ggttcgcgcc ttccgttggc gtcagttcgg
    39841 cttccttcag tgccagttgc agcatgtact gcgcttcttc ctggtcgtcc ttcaggtagc
    39901 gttcgtcgcg gccaaccttc accttgtaca gcggcggctg ggcgatgtac acatggccct
    39961 gctcgaccat cgacggcatc tgccggtaga acaacgtgag cagcagcgtg cggatgtgcg
    40021 cgccgtccac gtcggcgtcg gtcatgatga tgatgcggtg gtagcgcagc ttttccaggt
    40081 tgaagtcggg gccgatgcta gtacccagcg cggtgatcag ggtggtgatc tgttcgctgg
    40141 aaataagccg gtcgaagcgc gccttttcaa cgttcaacac cttgccgcgc agcggcagga
    40201 tggcctggaa cttgcggtcc cgaccctgct tggcggaacc gccggccgag tcgccctcca
    40261 cgatgtacag ctcgcacagc gcggggtctt tctcctggca gtcggccagc ttgccgggca
    40321 gcccggcgcc ttccagcacg ctcttgcggc gcgtcatttc gcgcgccttg cgcgcggcct
    40381 cgcgcgcgcg cgccgcgtcg atgatcttgc cgcacaacgc cttggcgtcg ttggggtttt
    40441 caagcaacca ggtttccagc gtgcgggcga cggcgtcttc caccgccggg cgcacttcac
    40501 tggacaccag cttgtctttg gtctggctgc tgaacttggg ttcgggcacc ttcaccgaca
    40561 acacgcaagt gagcccttcg cgcatgtcgt cgccggaggt gtcgaccttg gctttcttgg
    40621 ccagctcgtt gtcggcgatg tacttgttga tgatacgcgt catggcggcg cgcaggcccg
    40681 tcaggtgcga cccgccgtcg cgctgcggaa tgttgttggt gaagcacagc acggtctcgg
    40741 cgtagctgtc gttccattgc atggccacgt cgacacccac ggtggtgccg cccacggagg
    40801 actcggtgct gaccgcgaac acgttgctgt gcaggaccgt cttggcgcgg ttgatgtatt
    40861 cgacgaaccc cttgaccccg cccaggaagg cgaagctttc ctccttgccc tggcgctcgt
    40921 cgatgaggcg gatcttcacg ccattgttca ggaaggacag ctcgcgcagg cgcttggcca
    40981 ggatttcgta gtggtattcg atcttctcga agatgacggg gtcggccagg aactgcaccc
    41041 gggtgccacg cttttcagtg ggcccggttt cggccagcgg cgccacacgc tcgccctggc
    41101 ggaactcgat ttcgtgggtc ttgccctcgc gccagatggt caggcgcagc cattccgaca
    41161 acgcattcac gcacgacacg cccacgccgt gcagcccgcc cgacaccttg tacgaattgt
    41221 ggtcgaactt gccgccagcg tgcagctcgg tcatgacgat ttcggccgcg ctgcgatggt
    41281 attcgtcgtc tttgtgaatg ccggtgggaa tgccgcgccc gttgtcggtg acggaaatgc
    41341 tgtcatcgga atggatcatc accacgatgt catcgcaata gccggccagc gcctcgtcga
    41401 tggcattgtc gacgacctcg aacaccatgt ggtgaaggcc cgtgccatcg gacgtgtcgc
    41461 caatgtacat tcccggacgc ttacgcaccg cctcgagccc cttgagcatc ttgatcgagt
    41521 cggcgctgta tccggtggat tgggtctggg tgttctgatc tgacatgggt ggtgggtacc
    41581 ttatgtgatc ctggcattca tcggcgcgcg cccggaagcc gttccggccg cgccgcgagg
    41641 gttgttcgtg aaagtccgct agatgcgcat cggcatgacg acatacttga actggtcgtc
    41701 gccgggcagc gtgatgagca ccgacgcgtt gacgtcgggc tgcaccgacc attgcaccgt
    41761 ttcggtcttg acgttgccca gaacgtcgag caggtagtgc acgttgaagc ccacgtcgag
    41821 cggttcgtgg ccgtagtcga tttccagctc ttccagggcc tcttcctgct cggtgttgga
    41881 cgagctgatg gtgagctggt tctgcgccag ttgcacgcgc acgcccttga gcttgtcggt
    41941 ggtgagaatc gcggcgcgct gcagactgcc ctgcagcact tcgcggttaa tcgagaagtg
    42001 acgcgtgtaa ttggtgggga tgacgcgcgt gaagtcgggg aacttgccct cgaccagctt
    42061 ggacaccagc tcgacgtcgc cgaagcggaa gcggatctgc ccctgcgcga cgtcaacgga
    42121 aaccgggtct tcgctgtcgc ccagcaggcg ctgcatctcc aggacggtct tgcgcggcac
    42181 gatcacgtcg tggcgcgccg tgatgccttc gacagccgtg ccggtgtgcg ccaggcggtg
    42241 cccatcggtg gcgaccgcgc gcacatgacc gggttcgaaa acgaacagca aaccgttcag
    42301 gtagtagcgg atatcttgct gcgccatggc gaagtgcacc atgttgaaca agtgcttgag
    42361 cttggattgc ggcagcgaga acgacacatc ccaggtctgc ggttgcgaca cggcggggaa
    42421 atcggacgcg ctcatggttt gcagcgagaa gcggctcttg ccggactgca ccagcagctt
    42481 ggcgccggaa agctcgaact tcacgtcgga atcgggcagg gcgcgcagga tgtcgagcag
    42541 cttgcgcgcg gcgaccgtgg tggcctcggc accattaccc gtgccgatgc cggcctgggt
    42601 ggatatctgc acttcgatgt cggtcgcaat gaacgtcaca ctttcgcctt ctttgcgcaa
    42661 caggatgttg gccagaatgg gcagcgtgtt gcgccgttca acgatccccg ccacggtcgt
    42721 tagcggtttg agcagagcat cgcgagtcgt ctgtagaagt tgcatagtta tccttttagt
    42781 gtttgttcca atacgtgaag cgcgtggttg agttcagatt gcgtggagcg cgcgcccgtg
    42841 atcttgcgca cggcgtgcag tacggtggtg tggtcccggc cgccgaacag gtcgccgatt
    42901 tcgggcaggc tcttctgtgt gagctctttg gccaggtaca tggccacttg gcgcggcagg
    42961 gcgatattag ccggccgacg tttcgaatac atgtcggcga ccttgatctt gtagaagtca
    43021 gccaccgtct tctggatgtt ctcaaccgtg atctggccgt tcgacaccga cagcaagtct
    43081 ttcagggcgt ccttgcagac atcgaccgtg agcacctggc gcccgtggaa gcgcgcgtaa
    43141 gccgacacct tgcgcagcgc gccttccagc tcgcgcacgt tgctgcgcaa gtgcttggcg
    43201 atgaagaacg ccacttcctc gggcatggtg atgccttcgg tttccgcctt gcgcagcagg
    43261 atggccaccc gcatttccag ttccggcggc tcgatggcga ccgtcaggcc ggaatcgaac
    43321 cgcgaaatga ggcggctgtc tatgttggcc agctccttgg gatatgtgtc ggaggtgatg
    43381 atgatctgct tgcgctgggc caccatcgcc tcgaacgcat agaagaactc ttcctgggtg
    43441 cggttcttgc cggcgaagaa ctggatgtcg tcgatgagca gcagatcgag agaatggtag
    43501 tagcgcttga attcgtcgaa cgccttgcgc tggtacgcct tgaccacgtc ggacacgtac
    43561 tggtcggcgt gcacgtaccg cacgcgcgtg tcggcggaat cgcgcagcaa cgcattgccg
    43621 atggcatgaa tgaggtgcgt cttgcccagg cccacgccgc catacaggaa caacgggttg
    43681 tacgacacac cggggttctc ggccacctgc aacgcggcgg cgcgcgccaa ctggttggcc
    43741 ttgccggtga cgaaattgtc gaaggtgagg tccaggttca ggcgcgaccg atcctggccg
    43801 ggctgcgtca ccaccttgac tgtggggaca ggcgtggtgg cgggcgccgg gtcggcgccg
    43861 gcggggggtg tggcgatggc ggctggagcg tcggccggac gggccgccac cggccgggcg
    43921 gcgcccgcgg gcgcgctggc gagttcgaat tcgacctcga cgggccggcc ataccattcg
    43981 gcggccagcg atgcgatctg gcccgcatag ttgcggcgca cccagtcgag tttgaagcgg
    44041 ttgggggcgg acacacgcaa cacagcctgc gcttcatcga acgccacggg caccaggggg
    44101 cgaatccacg cgctgatctg ctgcgggggt agctcctgct ccaggcgttg caggcaggtc
    44161 tgccagaaat ctttcatgtc caccactttc acaaaccgcc attctacctc gtcggggggg
    44221 gagttatcca caagccaggc ggtttttgcc cggcatgcgg cgcccggaaa tttgactatt
    44281 caggggaaat ttgatgaaat ggtgggcttt tagtaagaat tcccacgccg atttggcctt
    44341 gggggttccg gggctgtgtg gtcatgttca cgcagtcgcc ggggcacgcg gggacgcgcg
    44401 cctttgcaaa gcggcctggt cgggcaagat tcgaaccggg cagggccgcc cacctccagg
    44461 ggggcggccg ggtttccaag tgttttccct agttcagtcg ttttcgccgc ctgcttgcaa
    44521 cagctggggg caaaccgcaa cgaccgggcg cgagcgtcgg ggcgaatccg ctccgcgggc
    44581 attgtccacg ggtcgccagg tcagctcttt tattttcgga tccttccatg aaacgcacct
    44641 accaaccttc cgtcacccgt cgcaagcgca cccacggttt ccgtgtgcgc atgaaaacgc
    44701 gcagtggccg cgccgtcctc aacgctcgcc gcgccaaggg gcgcaagcgt ctggccgtct
    44761 gagcacccgc tccggggatt cgtccccacc gcccgcaccg gcgcaatcaa ccatgagcgc
    44821 ctcgtttccc gccacggcgc gtctgcatcg tccctcggaa tacgccctgg ccctcaaagg
    44881 caaacgtgtt gccagagggg cgctgcttgt cgtgaccacg cccaggcagg tttctgacga
    44941 agcggtgcca gtccggcttg gcctgatcgt ggccaaacgg ttcgccgccc gggcggtgac
    45001 ccgcaacacc atcaaacggg tcatccgcga aaccttccgc aggaagcagc acgaactccc
    45061 ccagcgcgat ctggtctttc gcctgcacag caaggttcag gatctctcgc tcacggaact
    45121 tcggacgctg atccgggccg aaaccgaatt cctgctcgag cgagcccggt catgatcaag
    45181 tacctgctga tcgcccccat ccggttctac cggtatttca tcagcccgct gctgggcagg
    45241 aactgccgtt tcacccccac gtgctccgcc tatgccatcc aggccatcca gacacacggg
    45301 gcgatcgccg ggctgtggct ggcgatcaag cggatcgggc gctgccaccc atggtgccag
    45361 ggcgggtacg accccgtccc cgatgtggaa cacaaatggg gacacaagtg ccattaaggc
    45421 tttgtcggcc gatggcgctc cacgaggttt tgagcgaacg cttttgcttc ttcgcgggtc
    45481 tggccggcct acgttacact gactggcttt tttgctccgc accaacttga attcaggcgc
    45541 tatggatatc cgacgcaccg tcctctggat gatcttctct ttctcgctct tgctgttgtg
    45601 gaacaactgg caagtgcaca atggccagcc gtcgctcttc ggcctgggcc agacgagcac
    45661 ccatcaggcc gccaagcctg cggtgaaggc atcctccacc ccggtggctg acgccaatgt
    45721 gccggcctcg gccccaggcg aagccccggc ggccaagggc aacgccgccg tgcccggcaa
    45781 caaggccgcg ccggtcgcgc cgtcccagaa ggtgcaggtg cgcaccgatg tgtacgacct
    45841 cacgttcgac accaagggcg cccagctcat cggcgccaag ctgctgaaat acaaagccgt
    45901 cgacaacccc caacaaccgt tcacactgct cgacgactcc aaatccctga cctatctggt
    45961 gcaaagcggt gtggtcggcg cgccgaacgg ccagagctac cccacgcatc tgacgccttt
    46021 caagctcgtg tccaccgaca ccaccatgac aggcgacacg ctcaacgtcg tgttcgaggc
    46081 caccagcgac aacgtcaagg tggttcgcac ctacacgctg cgcaagggca gctacgacat
    46141 cggcgtcaag aacgccatct ataacctggg caccgagccc atctcgccgt cgctgtacct
    46201 gcagatcgtg cgcgacgaca gcgatccggc cagcgcctcg cacttcacgc acgcctacac
    46261 cggcacggcc gtgtattcca gcgaagacaa gttccagaag gccacgttct ccgacatcca
    46321 gaagggcaag gaccaataca tcaagaaggc gaacaacggc tggatcgcca tggtgcagca
    46381 ttacttcgcc acggcctgga tcccgcctga aggcgtggtc cgcacgaacc aggtggcgca
    46441 cctgtccaac gggctgtacg cgattcgcag catcctgccc gtgggcacca tcgccccggg
    46501 gcaggacaaa gtcaccaccg cccagctctg gaccggcccg caagaccaga aagccatggg
    46561 cgcggttgcg ccggggctcg atctggtcgt ggactacggc tggctcacca tcgtggccaa
    46621 gccgctgttc cagctcatgt cgctcctcca ttccctgttg ggcaactggg gctggaccat
    46681 cgtcgcgctc acgctgctga tcaaggccgt gttctacccg ttgtcggc
//
LOCUS       DASTNK010000038         3660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_581576, whole genome shotgun sequence.
ACCESSION   DASTNK010000038 DASTNK010000000
VERSION     DASTNK010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581576"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VFR20_03315"
     CDS             <1..816
                     /locus_tag="VFR20_03315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0229576.1"
                     /translation="ISPRISTYLWMESCRVRSRTGHRTVAVSGLIAQQLEQAYGPIAD
                     IQIIPPGVSAAPLPDASARAAQRHALGFSPDDRVCLLVARNPLRKGLPVILKAMGQLP
                     GHVKLLVVGGDDTTRAYVRQHVAAEVMERVVVAGETSNVDAYYAVADLYVHPTLNDSY
                     GMAPLEAMAFGLPVIVSATPWCGLSQYIRHEQEALLLSQPEDPDELAGSITRILEDDS
                     LRRRLAQGGKDFSLTRGWPEIARQYLDLYHQAMAERRTPETSAEGGTNQGGNR"
     gene            complement(937..1353)
                     /gene="cueR"
                     /locus_tag="VFR20_03320"
     CDS             complement(937..1353)
                     /gene="cueR"
                     /locus_tag="VFR20_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HEU0229577.1"
                     /translation="MNIGQASKASGVSAKMIRYYEQIDLIPRASRGDSGYRAYAQTDI
                     HRLRFIRRARDLGFSVAEIGDLLSLWNDRSRQSADVKRLAKAHIAELEDRIDSLRQMA
                     DTLNTLIACCAGDERPDCPILANLEQSDEDAPALQH"
     gene            complement(1369..>3660)
                     /locus_tag="VFR20_03325"
     CDS             complement(1369..>3660)
                     /locus_tag="VFR20_03325"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU0229578.1"
                     /translation="DWKHAMITSNKHDAATLELAVEGMTCASCVGRVERALRAVPGVS
                     DASVNLASERATIHGVASTETLIAAVDKVGYGARAIDTLDLAGRDEATARKDAERDAL
                     KRDLLIATALALPVFILEMGSHMIPAMHAWVQATIGMEQSWVFQFLLTTLVLVFPGRR
                     FYAKGFPALFRLAPDMNSLVAVGTAAAYLFSVVATFAPGWLPAGTVNVYYEAAAVIVA
                     LILLGRFLEARAKGRTSQAIQRLMGLQVKVAHVVRGSQVIDIPIGDVAEGDVVEVRPG
                     ERVPVDGDVTDGQSYVDESMITGEPIPIGKAVGDSVTGGTVNQKGALRLCATAVGGQT
                     VLAQIIRLVEQAQGSKLPIQAVVDKVTLWFVPTVMGAALLTLLAWLIFGPSPALTYAL
                     VNAVAVLIIACPCAMGLATPTSIMVGTGRGAELGVLFRKGDALQLLKDARVVALDKTG
                     TLTEGKPALTDLETAEGFDRASVLALVAAAESRSEHPIARAIVEAASRDGIALSAPSV
                     FESITGMGVHTVVDRVKVDVGADRYMRSLGLDVAVFAQTAERLGGEGKSPLYAAIDGR
                     LAAIVAVADPIKSGTPEAIAALHRLGLKVAMVTGDNARTAQAIAARLGIDEVVAQVLP
                     AGKVDTIRQLKVEHGTVAFVGDGINDAPALAEADVGLAIGTGTDVAMEAADVVLMSGD
                     LQGVPNAIALSRATIGNIRQNLFWAFAYNTALIPLAAGALYPAYGVLLSPIVAAGAMA
                     LSSVFVLGNALRLRRFRPVLASA"
BASE COUNT          668 a         1270 c         1133 g          589 t
ORIGIN      
        1 atcagcccgc gcatatcgac ctacctatgg atggaatcct gccgcgtccg atcccgcaca
       61 gggcatcgaa ccgtcgcggt ctcggggctg atcgcgcaac aactcgagca ggcttacggc
      121 ccgatcgccg atattcagat catccctccc ggcgtgtcgg ccgcgccttt gccagacgcc
      181 tcggcacgcg ccgcccagcg ccatgcgctg ggtttctcgc ccgacgatcg ggtgtgcctg
      241 ctggtggccc ggaatccgct gcgcaagggc cttccggtca tcctgaaggc aatgggccaa
      301 ctgccggggc acgtgaaatt gctcgtggtc ggcggcgacg acaccacccg cgcgtatgtg
      361 cgccagcacg tggccgccga ggtgatggaa cgtgtcgtcg tggcgggcga aacgtcgaac
      421 gtggacgcct actacgccgt cgcggacctg tacgttcacc ccactttgaa cgacagctat
      481 ggcatggcgc ccctcgaagc catggcgttc gggctgccgg tcatcgtcag cgccacaccc
      541 tggtgcggac tctctcagta catccggcac gaacaggaag cgttgttatt gtcccagccc
      601 gaagacccgg acgagcttgc cggcagcatc acccggatac ttgaggacga ttcactgcgc
      661 cggcgcctgg cccagggagg gaaggatttc tccctcacgc gcggctggcc cgaaatcgct
      721 cggcaatatc tcgatctata tcaccaagcc atggccgagc gtcggacacc cgagacctct
      781 gcggaaggtg gcacgaacca gggtggcaac cgatagcgcc acacccggcg cctattttcc
      841 cgccttgcga aagatggtcg acccagtccg cacgcggccc gcgaaaaata gccgaccaag
      901 cccagacacg gcgggttcgc cccaaaagcg gtccgttcag tgttgaagcg ccggggcgtc
      961 ttcgtcggac tgttccagat tggcgagaat cgggcaatcg ggccgctcat ccccggcgca
     1021 gcaggcgatg agcgtgttca gcgtgtcggc catctgcctc aagctgtcga tccgatcctc
     1081 caactcggcg atgtgcgcct ttgccaggcg tttcacgtcc gcgctctggc gcgaacgatc
     1141 attccacaaa ctgagcagat cgccgatctc ggccaccgag aagcccaggt cgcgggcgcg
     1201 gcgtatgaag cgcagccgat ggatatcggt ctgcgcgtag gcccgataac ccgaatcgcc
     1261 ccggcttgcg cggggaatca ggtcgatctg ctcgtaatag cggatcatct tggcggagac
     1321 gcccgatgcc ttggaagcct gtccgatatt catttcgaaa atccttgttc atgcggaagc
     1381 cagcactggc cggaatcgac gcagacgcag cgcgttgccc agcacgaaca cgctggacaa
     1441 cgccatcgcg cccgccgcca caatgggcga gagcaacaca ccataggccg gatacagcgc
     1501 gccggccgcc agaggaatca acgccgtgtt gtaggcgaac gcccagaaca ggttctggcg
     1561 gatgttgcca atggtggccc gggacagggc aatcgcattg ggcacgcctt gcaggtcgcc
     1621 agacatcaac accacgtcgg cggcttccat cgccacatcg gtgccggtgc cgatcgccag
     1681 ccccacgtcc gcctcggcca gcgcgggggc atcattgatg ccgtcgccca cgaaggccac
     1741 ggtgccgtgc tccaccttga gctgccggat cgtgtcgacc ttgccggccg gcagcacttg
     1801 ggccacgacc tcgtcgatac ccagtctggc cgcgatggcc tgggccgtgc gggcgttgtc
     1861 ccccgtgacc atggccactt tcaaacccaa ccggtgcagc gccgcgatcg cctcgggcgt
     1921 gccggatttg atgggatcgg ccactgcgac gatcgcggcc agacgtccgt caatggcggc
     1981 gtacagcggt gatttaccct cgccccccaa ccgctcggcg gtctgcgcga acaccgccac
     2041 atccagtccc agggaacgca tgtaacgatc cgccccgaca tccaccttga ccctgtcgac
     2101 cacggtgtgc acgcccatgc cggtgatgga ctcgaacacg ctgggcgcgg acagtgcgat
     2161 gccgtcccgc gatgcggcct cgacaatggc ccgggcgatc gggtgctccg acctcgactc
     2221 ggcggcggcc acgagggcca acaccgacgc gcgatcgaag ccctcggcgg tttcgagatc
     2281 ggtcagggcc ggctttccct cggtcaacgt gccggtcttg tccagggcca ccacgcgggc
     2341 gtccttcagg agctgcagag cgtcgccctt gcggaacaac acgcccaatt ccgcgccgcg
     2401 tccggtgcca accatgatgg acgtgggcgt cgccaacccc atcgcgcagg ggcaggcgat
     2461 gatgagcaca gccacggcat tcaccagcgc gtatgtcagc gccggcgatg gcccgaagat
     2521 cagccaggcc agcagggtga gcagcgccgc gcccatcacg gtgggcacga accacagcgt
     2581 gactttgtcc accacggcct ggatgggcag cttggacccc tgagcctgct cgaccaggcg
     2641 gatgatctgc gccagcacag tctgcccgcc gacggcggtg gcacacaacc tgagcgcgcc
     2701 cttctggttg accgtgccgc cggtcaccga atcacccacg gcctttccaa tcgggatcgg
     2761 ttcgccagtg atcatggact catcgacata actctgccca tccgtcacgt cgccatcgac
     2821 cggcacgcgc tcgcccggcc gcacttcgac cacatcgccc tccgccacgt ccccgatcgg
     2881 gatgtcgatg acctgggagc cacgcaccac atgggccacc ttcacctgca ggcccatcag
     2941 ccgctggatg gcctgggacg tacgcccctt ggcgcgcgct tccagaaaac ggccaagcaa
     3001 aatcagcgcc acgatcacgg cggccgcttc gtaatagacg ttgacggtgc ccgccggcaa
     3061 ccaccccggc gcgaaagtgg cgaccaccga gaagagatac gccgccgccg tgcccaccgc
     3121 caccagggaa ttcatgtctg gcgcgagacg aaacagcgcc ggaaagccct tggcgtagaa
     3181 gcgccgcccg gggaacacca gcaccagcgt ggtcagcagg aattggaaca cccagctttg
     3241 ctccatgccg atggtggctt gtacccatgc atgcatggcg ggaatcatgt gcgagcccat
     3301 ctcgaggatg aacaccggca gcgccagcgc ggtggcgatg agcagatcgc gtttgagcgc
     3361 gtcgcgctcg gcgtccttcc tggcggtggc ctcgtcacgg ccggccaggt cgagcgtgtc
     3421 gatggcacga gcgccataac ccactttgtc gactgcggcg atgagtgtct cggtggacgc
     3481 cacaccatga atggtggcgc gttcgctcgc gaggttcacc gaggcgtccg acacgccagg
     3541 cacggcccgc agggcacgtt ccacgcggcc cacgcacgag gcgcaagtca tgccctccac
     3601 cgcgagttca agcgtggcgg cgtcatgttt gttcgatgtg atcatggcgt gcttccagtc
//
LOCUS       DASTNK010000039         8055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1139347, whole genome shotgun sequence.
ACCESSION   DASTNK010000039 DASTNK010000000
VERSION     DASTNK010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8055
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139347"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..925
                     /gene="rsgA"
                     /locus_tag="VFR20_03330"
     CDS             2..925
                     /gene="rsgA"
                     /locus_tag="VFR20_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU0229579.1"
                     /translation="MPKATPDSRPTLLQGLITAAHGRHYMVELADGTLRTCFPRGKKA
                     GAAVGDKVLISPQGQREGTIETILERRNLLYRSDEFRTKQFAANVDQLLFVVAVEPAF
                     SDDLLGRALVAAASANIEPLIILNKVDLDHGASAARERLRYPAHLGFRVLDLSARDVD
                     AARRQLLPSLEGRTSLLLGQSGMGKSTLLNALIPEARAHTQEHSQALGAGRHTTTSTR
                     LYHLPDHLGDIIDSPGFQAFGLYHLGMDDIERGFPEIRAAREHCRFYNCTHRHEPGCG
                     VLEAVKRGDIPVARHALYERILAENEARNHY"
     gene            complement(1008..1754)
                     /locus_tag="VFR20_03335"
     CDS             complement(1008..1754)
                     /locus_tag="VFR20_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEU0229580.1"
                     /translation="MSSSSSVSRTPRSIVTPGFDPDAQPVISSKALAPLQAATLELDF
                     IRAAFNCPVEWVVEPVFAKSFSDGAAHEDVTHAAVFIALVQRLSGLHVIFTRRSPHLN
                     DHAGQISFPGGRIEPDDPDAVAAALRETREEIGIDSQFVEVIGTQPCFLTSTRFTMKP
                     VIGVVRPGFSITPNLTEVAEVFEVPLSVLMDTRLHRLHEARLPDGGHRFYFSISWRAY
                     FIWGATAALVRNLYHYLAAAQGVLGTAPQS"
     gene            complement(1994..2383)
                     /gene="rplS"
                     /locus_tag="VFR20_03340"
     CDS             complement(1994..2383)
                     /gene="rplS"
                     /locus_tag="VFR20_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU0229581.1"
                     /translation="MNLIEQLEQEEIARLTGGQAVPEFAPGDTVVVSVNVVEGSRKRA
                     QAYEGVVIAKRNRGLNSSFIVRKISSGESVERTFQLYSPQIASIEVKRRGDVRRAKLY
                     YLRSRAGKAARIKEKLVRGAGKKAAAQ"
     gene            2628..3062
                     /locus_tag="VFR20_03345"
     CDS             2628..3062
                     /locus_tag="VFR20_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229582.1"
                     /translation="MAIQKPLAAPSHIKPGMRAAQTTTRQISTAMAPRVGRGGALRAG
                     AGGKGHFMGVSRGVVVFMVASVEKMDRYGGKHNAPALHQANDRKLRPDTVHKNSYSIF
                     NCTKQQYQYRATTDGAAPAKNAPRRAHHEKTQPGAGHEEASR"
     gene            complement(3131..3769)
                     /locus_tag="VFR20_03350"
     CDS             complement(3131..3769)
                     /locus_tag="VFR20_03350"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEU0229583.1"
                     /translation="MTLRLGDTAPDFEQDSSIGKIRLYDYLGDKWGVLFSHPADFTPV
                     CTTELGYTAILSDEFAKRNVKVLAVSVDDADSHRGWIKDIDDTQRTTVNYPILADSDH
                     KVSNLYDMIHPNASTTATVRSVFIIDPARKVRLTITYPASTGRNFNEILRVIDSLQLT
                     DNYSVATPVNWEDGDDVIISPAIKDPAVLKQKFPKGYTELRPYLRVTPQPNR"
     gene            complement(3910..5727)
                     /locus_tag="VFR20_03355"
     CDS             complement(3910..5727)
                     /locus_tag="VFR20_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEU0229584.1"
                     /translation="MAYRVPTQDIRFVFKELAGLDDILALPGFEEMSDELIDAVMEEN
                     ARFVEQAVAPLNRNADTDPARWNNGAVVTTPGFRQAFHEYAQGGWQGLQHDPKWGGQG
                     LPKMVAAAAGENINAASLAFSLCPLLTDGVIEALATVGDKAQQDRFIPALIEGRWTGT
                     MNLTEPQAGSDLALVSTRAVHQGDGSYRLSGQKIFITYGEHDLAENIIHLVLARTPDA
                     PAGVKGISLFIVPKVLVGDDGSLGARNDVWCASLEHKLGIHGSPTAVLLYGSGKGEVG
                     EGAVGYLVGQENHGLEYMFIMMNAARYAVGIQGVAVSERALQKAQEYAFERVQGRALE
                     GSPGAVAIARHPDVQRMLMTMRALTEGARALAYVAAGELDRSRHHADPAARERSRQRY
                     EYLVPIVKGFSTETAVEVASLGIQVHGGMGFIEETGAAQYYRDARILPIYEGTTAIQA
                     NDFIGRKTLRDAGAVARGMAQDMLATAAELTAASPKGYEEGLGLIARQLEAAVQAYTH
                     ATDFMLANAANEVRAVFFGSVPYLMLAGVAHAGWHMARGALASARRLAAGEDDAFLRR
                     KFATGVFYAGHILPRAQALSVAVIEGQVVSRLTNQVYSA"
     gene            complement(5879..6808)
                     /locus_tag="VFR20_03360"
     CDS             complement(5879..6808)
                     /locus_tag="VFR20_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU0229585.1"
                     /translation="MTTLVIAEHDNAQLKGATLNTVAAAARLGGDVHVLVAGGGARAV
                     AEQAAKVAGVAKVLLADAPQLAEGLAENIAAQVLAVAQGHSHILFAATAAGKNVAPRV
                     AARLDVAQVSDIIAVDSPDTFQRPIYAGNAIATVQSGDAVKVLTVRTTGFDAVAAEGG
                     SATIEVVDAVADSGLSTFEGREVAKSDRPELAGAAIVVSGGRGLGSAENFKLLDPLAD
                     KLGAALGASRAAVDAGYAPNDWQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIV
                     AINKDAEAPIFGVADYGLVADLFQAVPELTAAL"
     gene            complement(6808..7557)
                     /locus_tag="VFR20_03365"
     CDS             complement(6808..7557)
                     /locus_tag="VFR20_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEU0229586.1"
                     /translation="MKVLVPVKRVVDYNVKVRVKSDQSGVDIANVKMSMNPFDEIAVE
                     EAMRLKEKGAAQEVVAVSCGAQASQETLRTALAIGADRAVLVQTDVELQPLAVAKLLA
                     AVVRKESPGLVILGKQAIDDDANQTGQMLAALLDWPQATFASKVEMGEGSVRVTREVD
                     GGLETLSLKLPAIVTTDLRLNEPRYVTLPNIMKAKKKPLETATPEELGVDVAPRLRTL
                     RVTEPPSRSAGVVVPDVATLVDKLKNEAKVI"
     gene            complement(7720..>8055)
                     /locus_tag="VFR20_03370"
     CDS             complement(7720..>8055)
                     /locus_tag="VFR20_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEU0229587.1"
                     /translation="VRLLELLDAARDEGVISGKYVAIARAMEVSLARLVGVKLPINID
                     GATAAVYCELGFPAALGRGLFILSRSVGVLAHAYEQSTLSERIKGPMPPSVPYSYVGP
                     AHRNLDGAS"
BASE COUNT         1539 a         2660 c         2437 g         1419 t
ORIGIN      
        1 catgcctaag gcaacgccgg actcccgtcc cacgttattg caaggcctga tcacggccgc
       61 ccacgggcgc cattacatgg tcgaactcgc cgacggaacc ctgcgcacct gcttcccccg
      121 gggcaagaag gcgggggccg cggtggggga caaagtgttg atctcaccgc agggacagcg
      181 ggaaggcacg atagaaacca tcctggagcg ccgcaacctg ctgtatcgct ccgacgaatt
      241 ccgcaccaag cagtttgctg cgaacgtcga ccagttgctg ttcgtcgtgg ccgtcgagcc
      301 ggctttctcg gacgacctcc tgggcagggc gctggtggcg gccgccagcg cgaacataga
      361 accactgatc atcctcaaca aagtcgatct tgaccacggc gcgtcggccg cgcgcgagcg
      421 cctgcgctat cccgcgcacc tgggttttcg cgttctggac ctgagcgcgc gcgatgtcga
      481 cgccgcacgc cggcaattgc tgcccagcct cgagggcagg accagcctgt tactggggca
      541 aagcggcatg ggcaagtcga cgctgctgaa cgccctcatt cccgaggcac gggcgcacac
      601 ccaggaacac tcccaggcac tgggagccgg tcggcacacc accaccagca cacgtctcta
      661 ccatttgccc gaccacctgg gggacatcat cgattccccc ggattccagg cctttggcct
      721 ataccacctg ggcatggacg acatcgagcg tggtttcccg gaaatccggg cagcccggga
      781 gcattgccgc ttctacaact gcacccacag gcacgaaccg ggctgcggcg tgctggaggc
      841 cgttaaacgc ggcgacatcc cggtggcgcg gcatgcgctg tacgagcgca tcctggccga
      901 gaatgaggcc cgaaaccact attgaggcaa agcgtggccg ctgtcggctc gagcgccgat
      961 tcgaagcgac tcggtgaatc acttcggttt cgcgggggga gggtcgatca ggattgtggc
     1021 gcggttccca gcacgccctg ggccgccgcc aggtaatggt acagattgcg caccagcgcc
     1081 gcggtggcgc cccagatgaa atacgcgcgc cacgagatgg agaaataaaa acggtgtccg
     1141 ccatccggca gcctggcctc gtgcagccta tgcaggcgcg tatccatgag caccgacagc
     1201 ggcacctcga agacttcggc cacctcggtc aggttgggag tgatcgaaaa cccgggccgc
     1261 accacgccta tgacgggctt catagtgaag cgggtggacg tcagaaagca aggctgagtg
     1321 cctatgactt cgacgaattg ggaatcgatg cctatttctt cccgggtttc gcgtaacgcc
     1381 gcagccacgg catccggatc gtcgggctcg atgcgccctc ccggaaaact gatctgcccc
     1441 gcatggtcgt tcaggtgcgg cgagcgccgg gtgaaaatga catgcaggcc ggacaaccgc
     1501 tgcaccagcg cgatgaacac cgcagcgtgc gtgacatctt catgggctgc gccgtcgctg
     1561 aatgacttgg cgaacacggg ttccaccacc cattccacgg ggcagttaaa tgcggcgcgg
     1621 atgaaatcca gctctagcgt cgcagcctgc aacggagcca gcgctttgga ggaaatgaca
     1681 ggttgggcgt cggggtcaaa gcccggcgtg acgatggagc gaggcgtgcg tgaaacagag
     1741 ctggatgacg acatgtggat ccaatctgcg cggcgacctg ggtgcgaccc cggacaccgc
     1801 actgttgccg atgccacatt ttactctgct tcgccttatg gaaaacccta ttgaccacta
     1861 ttggacaccg cacacccgcc caaaccggtt tcaacctatg acgaaagccg tgatgcattg
     1921 gccaaaaacg aatgggccgg gccccggttg caccgggccg gcccacgccg caagctggtg
     1981 acaggcgtcg cgattattgc gcggcggcct tcttgccggc tccgcgaacc agtttttcct
     2041 tgatgcgggc cgccttgccg gcgcggctgc gcaggtaata cagcttggcg cggcgcacgt
     2101 cgccacgacg cttgacttca atgctggcga tttgcgggga ataaagctgg aacgtgcgtt
     2161 caacggattc gccggaggaa atcttgcgaa cgatgaaaga cgaattcaag ccacggttgc
     2221 gcttggcgat aacgacgcct tcgtaggcct gcgcgcgctt gcggctgcct tcaaccacgt
     2281 tcacactgac caccacggta tcgcccgggg cgaattcagg cacggcctga ccaccggtga
     2341 ggcgcgcgat ttcttcttgc tcaagctgct cgatcagatt cactgaattg actccataag
     2401 tcatcatgaa cgggtaggcc cctattccct tggcctgtgt tggaaacgaa ggtcttgccg
     2461 accatcgcgc cccaggccag ccatggatga acgaatcccc atgggttttt cttgggaaac
     2521 ctatcgcccc gacagaggat gcgattgaaa accaagcatt atagcttgaa acacaccaca
     2581 ttgaaagcac gaaccaggtg gtgcgaaacg atacggtgcg ccgtgccttg gcgattcaga
     2641 agccgctggc ggccccaagc cacatcaaac ccggaatgag agctgcccaa acgacgacga
     2701 gacaaatatc gaccgccatg gctccccggg tgggccgcgg cggcgcgttg cgcgccggtg
     2761 cgggcggaaa aggccatttc atgggggtgt cgcgtggtgt ggtggttttc atggttgcct
     2821 cggtcgaaaa gatggatcgg tatggcggaa aacacaatgc ccccgccttg catcaagcca
     2881 acgacagaaa gctcaggccg gatactgttc ataaaaacag ttatagcatt tttaactgta
     2941 caaaacaaca gtatcagtat cgcgccacca cggatggcgc cgcgccggcc aagaatgcgc
     3001 cccgccgggc gcaccacgaa aaaacccagc caggggctgg gcatgaggag gcaagcaggt
     3061 gaaacgccag gccggtgtag tgtttgacgc gttagaggac caacaccgca ccagtgcccg
     3121 gcatcgtcgc tcaacgattc ggctggggcg tcacgcgcag gtacgggcgc agttcggtgt
     3181 agcccttggg gaacttctgc ttcagaacgg cggggtcctt gatcgcgggc gagatgatga
     3241 cgtcatcgcc atcttcccaa ttgaccggtg tcgccacact gtagttgtcg gtgagctgca
     3301 gcgaatcgat cacgcgcagg atttcgttga aattgcgacc agtgctggcg gggtacgtga
     3361 tggtcagccg caccttccgg gccgggtcga tgatgaacac cgagcgcacg gttgccgtgg
     3421 tgctggcgtt ggggtggatc atgtcgtaga gattcgacac cttgtgatcg gaatccgcca
     3481 gaatcgggta attgaccgtg gtccgctggg tgtcgtcgat atccttgatc cagccacggt
     3541 gcgaatcggc gtcatcgact gaaaccgcga gcactttgac gttgcgtttg gcgaattcgt
     3601 cggacaagat ggcggtgtag ccaagttcag tggtgcacac tggagtgaag tccgccggat
     3661 gggagaacag cacaccccat ttgtcaccca ggtagtcata aagacggatc tttccgatgg
     3721 aagaatcctg ctcgaaatca ggggcggtat cgcccagtcg caatgtcatg gcagtcactc
     3781 tcttgatcag ctggtcgtga agaacaagct ccgcgctgtg agggcggagc cttgtgaccc
     3841 ggaacgggtc aacgccagga cattgtgtca gcaacgaagt tgcgatggaa ataccgtttg
     3901 tgaatattct tatgcggaat aaacctggtt tgtcagtcgg ctaacaacct ggccttctat
     3961 gacagccacc gatagcgcct gggcacgagg aaggatgtgg ccagcgtaga aaacgcccgt
     4021 ggcgaatttg cggcgcagga aggcgtcatc ctcacccgcc gccaaccgac gcgcactggc
     4081 gagcgccccc ctggccatgt gccagcccgc atgcgcgacc cccgccagca tgagatacgg
     4141 aacgctgccg aaaaagacgg cgcgcacttc attggcggcg ttggccagca tgaaatccgt
     4201 ggcgtgcgta taggcctgaa ccgccgcctc taattgccgg gcaatgaggc cgaggccttc
     4261 ttcatagcct ttgggggacg ccgcggtcag ctcggccgct gtggcaagca tgtcctgggc
     4321 catgccccgt gccaccgccc ccgcgtcgcg cagcgtcttg cggccgatga aatcattggc
     4381 ctggatcgcc gtcgtgccct cgtatatggg cagaatgcgc gcgtcgcggt aatactgcgc
     4441 ggccccggtt tcctcgatga agcccatgcc gccgtgcacc tggatgccca gggacgcgac
     4501 ctcgacagcg gtttccgtgg aaaacccttt gacgatcgga accaggtact cataacgctg
     4561 acggctgcgt tcacgcgcgg ccggatcggc atgatgacgt gaccggtcga gttcgccagc
     4621 ggccacatac gccagcgcgc gcgcgccttc cgtcagggcc cgcatggtca tcagcatgcg
     4681 ctgcacgtcc ggatggcggg caatcgccac cgcgcccggc gacccttcca gcgcccgccc
     4741 ctgcacacgc tcgaacgcgt actcctgcgc tttctgcaac gcccgttcag acactgccac
     4801 gccttgaata ccgacggcat agcgcgccgc gttcatcatg atgaacatgt attccaggcc
     4861 gtgattttcc tgaccaacga ggtagccgac cgcgccctcg cccacttcgc ctttgcccga
     4921 cccgtacaag agcaccgccg tgggactgcc gtgaatgccc agtttgtgct ccagcgacgc
     4981 gcaccatacg tcattgcgcg cacccaggga gccgtcatcg ccgaccagga ctttgggcac
     5041 gatgaacaac gaaattccct tgactccggc aggggcgtcc ggcgtgcgag cgagcaccag
     5101 atggatgatg ttctcggcga gatcatgctc accgtaagtg atgaagattt tctggccgga
     5161 aagccggtag gaaccatccc cctgatgcac ggcgcgggtg gaaaccaggg caaggtccga
     5221 ccccgcttgc ggttccgtga ggttcatggt gcccgtccaa cgtccttcga tgagggcggg
     5281 gatgaagcga tcctgctgag ccttgtcgcc taccgtggca agcgcctcga tgacgccgtc
     5341 ggtcagcagg ggacagagcg aaaacgccag gctggccgcg ttgatgttct caccggcagc
     5401 ggcggccacc atcttcggca acccctggcc accccacttc gggtcgtgct gcaacccttg
     5461 ccaaccgccc tgggcgtact catggaacgc ctgacgaaag cccggggtcg tcacgaccgc
     5521 gccattgttc caacgcgcgg ggtcggtgtc cgcgttacgg ttcagaggtg ccacggcctg
     5581 ctcgacgaac cgggcgtttt cctccatgac cgcgtcgatg agctcgtccg acatctcttc
     5641 gaagcccggc agcgccagga tgtcatccag cccggcaagc tctttgaaaa cgaaacggat
     5701 atcctgtgtc ggtacgcgat aggccatgaa tcgtctcctg ggtcatgttg gcgcggcacc
     5761 attcattcgc gctggattcc gccccacggg gaagcgtcag cacatggggc aacgccgcca
     5821 cgcgcggcat tgccccgggc aggccggcga tccgccagcc ttgccccaac ctacagagct
     5881 acagggctgc ggtcagttcc ggcacggcct ggaacaaatc ggccaccagg ccgtagtcgg
     5941 ccaccccgaa gatgggcgcc tcggcgtcct tgttgatggc cacgatgacc ttggagtcct
     6001 tcatgcccgc caggtgctgg atggcccccg agatgcccac cgcgatgtac agctgcggcg
     6061 cgacgatctt gccggtctgc cccacctgcc agtcattggg cgcgtagccc gcgtccaccg
     6121 ccgcgcgcga ggctcccagc gccgccccca gcttgtcggc cagggggtcc agcagcttga
     6181 agttctcggc gctgccaagc ccccgcccgc ccgagaccac gatggccgca ccggccagct
     6241 ccggccggtc gctcttggcg acctcgcgcc cttcaaacgt cgagaggccg gaatcggcca
     6301 cggcatccac cacctcgatc gtggcgctgc cgccctcggc cgccaccgca tcgaaccccg
     6361 tcgtgcgcac cgtgagcacc ttgaccgcat cgccgctttg caccgtggcg atggcgttgc
     6421 ccgcgtagat gggccgctgg aacgtgtcgg gcgagtccac cgcgatgatg tccgagacct
     6481 gggccacgtc gagcctggcg gccacgcgcg gcgccacgtt cttgcccgcg gcggtcgccg
     6541 cgaacaggat gtggctgtgg ccctgcgcca ccgccagcac ctgcgccgcg atgttctcgg
     6601 ccaggccctc ggccaactgc ggcgcgtcgg ccagcagcac cttggccacg cccgccacct
     6661 tcgcggcctg ctcggccacg gcccgcgccc cgcccccagc gaccagcaca tgcacgtcgc
     6721 caccgagtcg cgccgccgcc gcgaccgtgt tcagcgtggc gcccttcaac tgggcgttat
     6781 cgtgttcggc aattaccagc gtcgtcatca gatcaccttc gcttcattct tgagtttgtc
     6841 caccagcgtg gccacatcgg gcaccaccac cccggccgag cggctcggcg gctcggtgac
     6901 cctcagcgtc ctcaggcgcg gcgcgacgtc cacgcccagt tcttcaggcg tggcggtctc
     6961 cagcggcttc ttcttggcct tcatgatgtt gggcagcgtc acgtagcgcg gctcgttcag
     7021 gcgcaggtcc gtcgtgacga tggccggcag cttcagcgac agggtctcca ggccgccatc
     7081 gacctcgcgc gtgacgcgca ccgagccctc gcccatctcg accttgctgg cgaacgtggc
     7141 ctgcggccag tccagcagcg cggcgagcat ctgcccggtc tggttggcgt cgtcatcgat
     7201 ggcctgcttg cccaggatca ccagcccggg cgactccttg cgcaccaccg cggccagcag
     7261 cttggccacc gccagcggct gcagctcgac gtcggtctgc accagcaccg cgcggtccgc
     7321 gccgatggcc agcgccgtgc gcagcgtctc ctggctcgcc tgcgcgccgc acgagaccgc
     7381 cacgacctcc tgcgccgcgc ccttctcctt gagccgcatg gcctcttcca ccgcgatctc
     7441 gtcgaacggg ttcatcgaca ttttcacgtt cgcgatgtcc accccgctct ggtccgattt
     7501 gacgcgaacc ttcacgttgt agtccaccac gcgcttgacg ggcaccagta ccttcatcca
     7561 ggggtcctcc agggattctc aaaaaaacga ggtttcgaca caccgtcgaa acctcgtacg
     7621 tcagtgaatg gttcgagcgt tctccaattc acaaggcaag tcgttaccac ggaggctcac
     7681 acaaacccga gccttctcgg caaactacgg gagccgctct caactcgcac catccaaatt
     7741 ccggtgcgcc ggcccgacat aggaatatgg aacactggga ggcatcggcc ccttgatgcg
     7801 ctcggacaag gtcgattgct cataagcatg agccaggaca ccgacggacc gcgacaagat
     7861 aaacagcccg cgaccgagag ccgccggaaa gcccagttca caataaacag cggccgtggc
     7921 cccatcgatg tttatcggca atttgacacc gaccaaccgc gccaatgaca cctccatcgc
     7981 acgggcgatc gcaacatatt tccccgagat cactccttcg tcacgggccg catcaagtaa
     8041 ctccaacagc cgaac
//
LOCUS       DASTNK010000040        10126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1509992, whole genome shotgun sequence.
ACCESSION   DASTNK010000040 DASTNK010000000
VERSION     DASTNK010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10126
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509992"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFR20_03375"
     CDS             complement(<1..362)
                     /locus_tag="VFR20_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU0229588.1"
                     /translation="MNTTKAAVAKPILKNLDRLIPDLEALYKDVHAHPELSMQEHRTA
                     ELAADRLRATGYDVTTGVGGTGVVGVLKNGDGPTVMMRADMDALPVEEATGVPYASKV
                     KGADADGQAVPVMHACGH"
     gene            complement(560..1414)
                     /locus_tag="VFR20_03380"
     CDS             complement(560..1414)
                     /locus_tag="VFR20_03380"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0229589.1"
                     /translation="MTIAITGSTGQLGRILIERLKASQSANSLVALARTPSKAANLGV
                     SVREADYTKPDTLVQALSGVDTLMMISASEIGQRVEQHRHIIDAAKKAGVRRIVYTSL
                     LRADTSPLEALADEHVQTERMLKESGILHTVLRNGWYTENYLGSIQGILAGNAVLGAA
                     AAGRISSAARADYAEAALVALSQPEHDGKIYELAGDQAYTLAELAAEIARQTGKPIVY
                     QNLNAASYAKKLQEFGLPTGFATAIADWDVGASKGGLFDDGHVLSRLIGHPTTPMSVA
                     VTQALKQV"
     gene            complement(1505..2911)
                     /locus_tag="VFR20_03385"
     CDS             complement(1505..2911)
                     /locus_tag="VFR20_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU0229590.1"
                     /translation="MKTTMCLKTILSSTLAALAVCLSLGAQAADTSDAKLIQQGKYLA
                     TAGDCIACHTARGGKPFAGGLAVSTPFGDIFSTNITPSKTAGIGNYTLEQFTRALREG
                     VRADGKHLYPAMPYTSYAQLSDADIKAMYTYFMHGVAPVDTPAPKTSLPFPFNIRLDM
                     LAWNFLFLDDKPYKPIPNKGYLWNRGAYLVQGLAHCTACHTPRNTFMAEDSSRSLGGA
                     YVGPWYAPNITSDSNSGIGGWSDKEIIEYLSTGHTAKAQASGPMMEAIDFSLRHLSKD
                     DLKAIAVYLKTVPAIHDAADTKPVYDWGSASDKLATVRGQPWPSNPDQMTGTQLYDAH
                     CATCHQAKAQGSFDDGLPALFHNTATARTQTNNLVLAILDGIHLPAQGNGVVMPGFRE
                     AMDDTQVTTLSNYLIKAYGNPAGKVTVEQVAALRSGAPTQASGVDLLLLARIGLVIAG
                     LIILLILYLIFRPRRRRA"
     gene            complement(2936..4573)
                     /locus_tag="VFR20_03390"
     CDS             complement(2936..4573)
                     /locus_tag="VFR20_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEU0229591.1"
                     /translation="MADSLNADFIVIGSGIIGSLTAMKLAQAGASVLILEAGPRYTRD
                     HIVANFRNSPRRSDWMSPYPFSPWAPNPIYKPQDNGYLVQAGPYPYPAEYIRMVGGTT
                     WHWAAHAWRNIPNDHRIRTLYGVGVDWPVSYDELEPFYQDAEEIMGVSGAPDTGSPRK
                     KPFPMEPVAEPYAMRRVRERLDPTYKVVTNTTARNSRPYDGRPACVGNNSCQPICPID
                     AQFNGGRAVKKAEDAGVKLVPNANVYKLESDDKGRVVAALYYDPNKGTHRVTGKTFIV
                     AANGIESPRLLLLSTSDKFPKGLANARDMVGRHLMDHPSTSLTFDADEDLWLGRGPQS
                     PSSINTMRDGEFRKEHAPYRLDFTNISRVDGTTKSLIADGVYGAEFERRLRHSAAREL
                     NVKTVLEVLPHRDNRITLSSQKDGMGIPKPEAHYFIDDYTHKGAIRALDDFRRIADLM
                     GGTNLRFTRDGEYANNQHICGTVRTGNDPATSVVDKWGRAYDHENLFMVGTGVIPTCG
                     TCNSTENGVALALHTVHHILGGQAGGSHAGDSARAKS"
     gene            complement(4583..5098)
                     /locus_tag="VFR20_03395"
     CDS             complement(4583..5098)
                     /locus_tag="VFR20_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar dehydrogenase complex small subunit"
                     /protein_id="HEU0229592.1"
                     /translation="MQEFSGPPDSLPPSPRRRRLLAGFVGAYTATLVPWAMAQPVKGD
                     QHGAFLALSAIIAGRQSLDADMADRIFRALSVEQSGFPASAAALLQLIDERKLDPMQL
                     QKTLDDEKSPLAPLPRKIATAWFLGVIGSGAQAHVIAYEDALNAQFVADVLKPPTYAY
                     GPYGSWTRKPL"
     gene            5470..6168
                     /locus_tag="VFR20_03400"
     CDS             5470..6168
                     /locus_tag="VFR20_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEU0229593.1"
                     /translation="MRGPSHCELPDGPLNYQDIIVIGKLVSAMKPVALPGRRIGNTLV
                     KSSATHYDSISQFLHWCTAVLVLVAFIYGPGGPEQRVYSSAHEFNRQLHETLGLCVFA
                     LTVIRLLWRAVATRPEAPPGARWMVVMATTVQWALYLLLLALPLTAIAGAWLEGHPLT
                     LLAGAEIRPLFGMSHGVGAAFASAHKWLGDAIMWLAGFHALAALFHHVVLKDDVLASM
                     LPRWLPDGWTKIGR"
     gene            complement(6344..7699)
                     /locus_tag="VFR20_03405"
     CDS             complement(6344..7699)
                     /locus_tag="VFR20_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006493301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0229594.1"
                     /translation="MRGSIRKRLTFLMLSGVALVWAYALYSSYHQAVDEVGEWDETRI
                     EQTARALLVLDARDLSAFARASLKPRDDDDDKISMRLLFEVTGSNGQILAASPGLSST
                     GAPLFLTGSANPGWTHKAKWHAYVIRDAGSGRTVRIFEQRTHRSELSTQVAQRIARPL
                     MFALPLLAVLIWFAIGSSLAPLRLLSKAIEERSPDGLEDIDVKGVPDEVQPLIVALNA
                     LLLRLRHSLDRERAFTSNAAHELKTPLSAIKVQAQVALTARDPDRQGRAMQRVVEAVD
                     RSTHLADQLLTLARMEETIPLPSAPMDLSDMTRACIADQLARADRKQMSLTMRADGAI
                     VVQTPPVLMRMLLDNLIDNAIKYGHVRGRVEISAWKDVDGTIYLQVRDDGPGVAAADL
                     PRLHDRFFRGANHGEQGSGLGLSIVARIVSQLRGKMEYTAGMEGRGFGVRIGLPSAAP
                     R"
     gene            complement(7696..8358)
                     /locus_tag="VFR20_03410"
     CDS             complement(7696..8358)
                     /locus_tag="VFR20_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0229595.1"
                     /translation="MRVLLVEDDNLIGSGVEDGLAEAGMSVDWARDGRQAQLALDTTP
                     YALVVLDLGLPRMSGTELLGWIRKRGDHTPVLVMTARDTVADRVSGLSAGADDYLGKP
                     FDLTELIARCRALVRRAQGRGTETIEYGDLLVDPERLMVMRGNERIALTSRECALLVD
                     LLANMGHPLSRARLQDSLYGWNEEIESNAIEVHVSNLRKKLGAKLIRTIRGVGYVMEN
                     MS"
     gene            8630..8905
                     /locus_tag="VFR20_03415"
     CDS             8630..8905
                     /locus_tag="VFR20_03415"
                     /inference="COORDINATES: protein motif:HMM:NF019008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HEU0229596.1"
                     /translation="MRDMKHAMRGALASQTMPDLSRHVARLESDAHRASRQLYRSDQS
                     TYDKGMRALRHELTEVDQAIQANNMKAAKRALRKINVTKRHYHHLLG"
     gene            8938..9522
                     /locus_tag="VFR20_03420"
     CDS             8938..9522
                     /locus_tag="VFR20_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEU0229597.1"
                     /translation="MKQVIRYPLSISLLHWLLAIALIGNLVIGWLLDDNEGLLDLHKS
                     IGIAILGLVLLRLVNRLRVRHQLPPSTNAAGTSAYRVEKLVHRLLYALMLAIPLLGWL
                     KTNAAGHAANCFGLFSLPTLMPRSHTLSPWLGQLHSLAAYSLAALVGLHVLGALAHWM
                     FKAENIFPRILPLPGRLARQKTSLGDRGRASQDA"
     gene            complement(9720..>10126)
                     /locus_tag="VFR20_03425"
     CDS             complement(9720..>10126)
                     /locus_tag="VFR20_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229598.1"
                     /translation="QDFRPETGSRVTMQPFDQRQVNFMNRSLLAAAMALAVTCSTPAL
                     AHVDIGIGIGIPGFGLGVPGVVVAPPAYYPPVEYVAPPPPPVVVVPPRPVYTPYYGWA
                     YPRYDHHDDHWRDRPRWRGHKWRHGDDDDQGD"
BASE COUNT         1904 a         2944 c         3129 g         2149 t
ORIGIN      
        1 tcatgaccgc aggcgtgcat caccggcacc gcctggccgt cggcgtcggc acccttgacc
       61 ttgctggcat agggcacacc ggtggcttcc tcgaccggca gcgcatccat atcggcgcgc
      121 atcatcactg tggggccgtc gccatttttc agcaccccca ccacgcccgt gccgccgaca
      181 ccggtcgtca cgtcataacc ggttgcgcgc aaccggtcgg ccgcgagctc agcggttcgg
      241 tgttcctgca tggacagctc gggatgcgca tgcacgtctt tgtatagggc ttcgaggtct
      301 ggaataagcc gatcaaggtt cttcaagatg ggcttggcaa ctgcggcttt cgtagtgttc
      361 atgactaccc tcgagcaatt cggatgaccg gcatcgagca acgtcggtat attttcaaag
      421 gagaaatgcc caactgacct gccacatctc gtctcttgct cacgccgtgc tcctttgatg
      481 gaaagtcatg gttgtcatcg cacggacact atccaggaga tctgacccgc gcgtgtgaag
      541 ggctgatcgt gtgagcacgc tatacctgtt tcagagcctg ggtaacagcc acggacatgg
      601 gtgtcgttgg gtgccctatg agccttgaga gcacgtgacc gtcatcgaac agaccgccct
      661 tggatgcgcc aacgtcccaa tcagcaatgg ccgtagcaaa ccctgtgggc aggccaaact
      721 cctgaagttt cttcgcatag gatgcggcat tcaggttttg ataaacgatg ggcttgcccg
      781 tttgcctggc gatctccgcc gccagctcgg cgagggtgta tgcctggtca cccgccaatt
      841 cataaatctt gccatcgtgt tccggctgcg acagcgcaac caacgcggct tcggcatagt
      901 cggcccgcgc ggcagaagaa atccggccgg cggcagctgc ccccaagacg gcattgccgg
      961 ccaggatgcc ctggatcgag cccaggtagt tttcggtgta ccaaccattg cgcaagacgg
     1021 tgtggagaat gccagactct ttcaacatgc gctccgtctg gacatgctcg tctgccaggg
     1081 cttcgagagg ggaggtgtcg gcccgtagca agctggtata gacgatgcgg cgtacccctg
     1141 ctttttttgc ggcgtcgata atgtggcgat gttgttcgac gcgttggccg atttcgcttg
     1201 cggaaatcat catcagcgtg tcgacgcccg acaatgcctg aacgagcgta tctggcttgg
     1261 tgtaatccgc ctcacgcacc gaaacgccca ggttggcggc cttggagggt gtgcgtgcaa
     1321 gcgcgaccaa actgttggcg gattgggagg ccttgagcct ttcgatcagg atgcggccga
     1381 gttggcctgt ggagcctgtg atggcgatag tcatgatgga ggatcctcgt tgagtcaatt
     1441 tagcaaccca cattgcatcg aacaacatgg gggccttgta accgtgggac aatacatcgc
     1501 ggggtcacgc ccgacgccga cgcggccgaa aaatcaaata aagaatcaac agtatgatga
     1561 ggcccgcgat caccaaaccg atacgcgcga gcaataacaa atccaccccg gaggcttggg
     1621 tgggggcgcc gcttctcagg gcggcgacct gttcaaccgt gactttgcca gccgggttgc
     1681 cataggcctt tatcagatag ttgctcaggg tcgtcacctg cgtgtcgtcc atggcctcgc
     1741 gaaagcccgg catgacgacg ccgttgccct gcgccggcag gtgaatgccg tcgagtatgg
     1801 ccaggaccag attgttggtc tgagtacgcg cggttgccgt gttgtggaac agcgctggca
     1861 ggccgtcgtc gaaactgccc tgggcctttg cctggtgaca agtggcgcag tgcgcgtcgt
     1921 aaagctgcgt gccggtcatt tgatctggat tcgacggcca gggctggccg cgcacggtgg
     1981 ccagcttgtc ggaggcgctg ccccaatcgt agacgggttt ggtatccgcg gcatcgtgaa
     2041 tggccggcac tgttttcaga taaaccgcga tagctttcaa gtcgtccttc gacaggtgcc
     2101 tcaggctgaa gtctatggcc tccatcatgg ggccagaggc ctgggctttg gctgtgtggc
     2161 ccgtgctcag gtattcgatg atctccttgt cgctccagcc gccaatgccg ctattggaat
     2221 cagaggtgat gttgggggca taccaggggc cgacgtaagc gccgcccaga gaacgcgagg
     2281 agtcctccgc catgaacgtg ttgcgaggcg tatgacacgc cgtgcaatgc gccagaccct
     2341 ggacaagata ggctccgcgg ttccagaggt agcctttgtt gggaatcggc ttatacggtt
     2401 tgtcgtcgag gaacagaaaa ttccaggcca acatatccag gcggatattg aacggaaagg
     2461 gcagcgatgt cttgggcgcc ggggtgtcga ccggcgcgac gccatgcatg aaataggtgt
     2521 acatcgcttt gatgtcggcg tcgctgagtt gcgcgtagct ggtatacggc atggctgggt
     2581 acaaatgctt gccgtcggca cgcacgcctt cgcgcagtgc gcgggtgaat tgttcgagtg
     2641 tgtagttgcc tataccggcc gttttagacg gcgtgatgtt ggtggaaaaa atgtcgccga
     2701 acggtgtgga aaccgccaag ccgccggcga aaggtttgcc tccccgggcg gtatggcagg
     2761 ctatgcagtc accggccgtg gccaggtact tcccttgctg gataagcttg gcgtcgcttg
     2821 tgtcggcagc ctgggcaccc aggctcaggc agacggccag cgccgctagg gtcgaggaca
     2881 gaatggtttt caaacacatc gtcgttttca tggggacccg cctttacagg gatggtcaag
     2941 actttgcccg ggcagaatcg ccggcgtgcg aaccgccggc ttgcccgccc agaatgtgat
     3001 gcacggtatg cagcgccagg gccacgccgt tctcggtgga gttgcaggtg ccgcaggtgg
     3061 gaatgacccc ggtgccaacc atgaacagat tttcgtggtc gtacgcgcgc ccccacttgt
     3121 cgacgacgga ggtggcggga tcattacccg tgcggacggt tccgcatata tgctggttgt
     3181 tcgcatactc gccgtcgcgc gtgaagcgca ggttggtgcc ccccatgaga tcggcgatgc
     3241 gacggaaatc gtccagagcg cgaatggcgc ccttgtgtgt gtagtcgtcg ataaagtagt
     3301 gagcttcagg cttgggaata cccatgccgt ctttttgcga gctgagggta atccgattgt
     3361 cgcggtgggg cagtacctcg agcaccgttt tcacattcag ttcccgcgcc gcgctgtgcc
     3421 gcaggcggcg ctcgaattcg gcgccataga ccccgtcggc gatcagcgac ttggtggtgc
     3481 cgtcaacccg ggatatgttc gtgaagtcca gtcggtaggg cgcgtgctct ttgcggaact
     3541 cgccatcgcg catggtgttg atcgagctgg ggctttgcgg gccacggccc agccagaggt
     3601 cttcgtcggc gtcgaaagtc agcgacgtgc tggggtggtc catgagatgc cggcccacca
     3661 tgtcccgcgc gttggccagg cctttgggaa atttgtcgct ggtggaaagc aggagcagcc
     3721 gcggactttc gatgccgttg gccgcgacga tgaaggtttt gcccgtgaca cggtgcgtgc
     3781 ctttgttggg gtcgtagtat agcgcggcca ccacgcgccc tttgtcgtcg ctttccagct
     3841 tgtagacgtt ggcgttgggc accagcttga ctccggcgtc ttccgctttt ttcacggcgc
     3901 gcccaccgtt gaattgcgca tcgatggggc agatgggctg gcaactgttg ttgccgacac
     3961 aggcggggcg cccgtcgtag ggccggctgt tgcgcgccgt ggtgttggtg acgaccttgt
     4021 aggttgggtc cagtcgttcg cgcacacgcc gcatggcgta gggctcggcc accggttcca
     4081 tggggaaggg cttcttacgg ggcgacccgg tgtcgggggc gccagatacg cccatgattt
     4141 cctcggcatc ctggtagaag ggttccagct cgtcgtagga aaccggccag tccaccccga
     4201 cgccgtacag cgtgcggatg cggtggtcgt tggggatatt gcgccaggcg tgcgccgccc
     4261 agtgccaggt ggtgccgccc accatgcgta tgtattccgc tgggtacggg taggggcccg
     4321 cttgtaccag gtagccattg tcttgcggct tatagatggg gttgggcgcc cagggggaga
     4381 acgggtaggg cgacatccag tcgctgcgtc gaggcgagtt gcggaaattc gccacgatgt
     4441 gatcgcgcgt gtagcggggg ccggcctcga gtatgagaac cgacgcgccc gcctgggcaa
     4501 gcttcatggc cgtgagggaa ccgatgatgc cggagccgat cacgatgaaa tccgcgttca
     4561 gagagtctgc catggggttt ccttacaagg gcttgcgcgt ccaactgcca taggggccat
     4621 aggcataggt gggaggcttg agcacatcgg cgacgaactg cgcgttgagc gcatcctcgt
     4681 atgcgatgac gtgtgcctgg gcaccggagc cgatcacacc aagaaaccac gcggtcgcga
     4741 ttttgcgcgg caagggggca agtggtgatt tttcatcgtc gagcgtcttt tgcaactgca
     4801 tcggatcgag cttgcgctcg tcgatcagtt ggagcaatgc ggcggcggag gcgggaaacc
     4861 ccgactgttc gacgctgagc gccctgaaga ttcgatcagc catgtcggcg tccagcgact
     4921 ggcgccctgc gatgatggcc gacaaggcca ggaaggcacc gtgctggtcg cccttgacgg
     4981 gttgggccat tgcccaggga accaatgtgg cggtgtatgc gccgacgaat ccggcgagca
     5041 ggcgcctgcg gcgaggtgat ggcggaagtg agtctggcgg accggagaat tcttgcacgg
     5101 ttcgatctcc ttcttggtgt gtctcaacca accaactgaa ctggccaacc caacccaacc
     5161 caacccgtta ttcttgacta cccgaggctt tcggccggga agcggtatag gcattctgat
     5221 tgtctctctt agttctgagt acgcacaaaa aagaaaatga aacaaaacat aacatcgcat
     5281 tcatgcgtgc ttgacggagc agactgaaat gcgagttttg tcgcttcccg gttttgttta
     5341 ttggtagatc accataaaac gcataatgta tattatgtaa aattaaagat tcaaaaaata
     5401 atttgtggat tcacctgtcg tcgttccaca aagaagccga gttcgggagg cccatgatgg
     5461 ctggttgtga tgcgcggccc gtctcattgt gaattgccgg acggcccatt aaactaccag
     5521 gacattatcg tcataggcaa actggtcagc gcaatgaaac ctgttgccct tccggggcga
     5581 cgcatcggaa acactttggt gaaaagctcc gccacccatt acgattcaat ctctcaattc
     5641 cttcattggt gcacggcggt gcttgtcctc gttgcgttca tatatgggcc gggagggccc
     5701 gagcagcgtg tctattcatc cgctcacgag ttcaaccgtc aattgcatga aaccctgggg
     5761 ttgtgcgtgt tcgcgctcac cgtcatccgt ttgctgtggc gggcggttgc cactcgcccc
     5821 gaggcgcctc cgggcgctcg ttggatggtt gtgatggcga cgactgttca atgggcactg
     5881 tatctgctgc tgctcgccct tccgctgacc gccatcgctg gcgcgtggct cgaagggcac
     5941 ccattgacgc ttctggccgg ggccgagatt cgaccattgt tcggtatgtc tcacggcgtg
     6001 ggcgcggcct tcgcgtcggc ccataagtgg cttggcgacg cgatcatgtg gcttgccgga
     6061 tttcacgcac tggcggcgct tttccatcat gtcgttctca aggatgacgt gctggcctcc
     6121 atgcttcctc gttggctgcc cgatggatgg acaaagattg gccggtgagt cctcctgtcg
     6181 gccaggacga ctgccttgtc atcgcaaaca gtcatcagtc tttaaaacga aacaaccgtc
     6241 gggaatcggc gtcataggtt gccggtagcc gccaaacgcg gtcgacaccg gatgtggtgc
     6301 gatacgcggc acgcgcgagg tgcctggtgt gcctgcggac gggctaacgg ggggcggccg
     6361 agggcaggcc gatgcgcacg ccgaagcccc ttccttccat tccggccgtg tattccattt
     6421 tcccgcgtaa ttgggacaca atgcgtgcaa cgatcgatag gcccagtccg cttccctgct
     6481 cgccatggtt ggcgccccga aaaaaacggt cgtgcaggcg aggcagatcc gccgcggcaa
     6541 cgccggggcc gtcgtcgcgc acctgcagat aaatagtgcc gtcgacatct ttccatgccg
     6601 aaatctcgac cctgccccgc acatgcccat acttgatcgc attgtcgatg agattatcga
     6661 gcagcatgcg catcagcacg ggtggtgtct gcacgacgat tgcgccgtca gcccgcatcg
     6721 tcaacgacat ctgcttgcga tccgcccggg caagctggtc ggcgatgcat gccctggtca
     6781 tatccgagag gtccattggc gcgctgggca gcggtatggt ttcctccatt cgggccaagg
     6841 taagtaactg gtcggctaga tgcgtgcttc gatcaacggc ttcgaccacg cgctgcatcg
     6901 cgcgcccctg gcgatcgggg tcgcgcgctg tcagggcaac ctgcgcctgg actttgatcg
     6961 ctgagagtgg tgtcttgagt tcatgggcgg cgttgctggt gaacgcgcgt tcacggtcaa
     7021 gcgagtggcg caagcgcagc agcagcgcat tgagcgcgac gatcagcggt tgaacttcat
     7081 cgggtacccc tttgacatcg atgtcttcga ggccatcggg cgatcgttcc tctatggcct
     7141 tggacagcaa tcgcaaaggc gccaggctgc tgccgatcgc gaaccagatc agcaccgcca
     7201 ggagtggcag tgcgaacatc agtggtctgg caatacgctg cgcgacctgg gtcgatagct
     7261 cggaacggtg cgtgcgctgc tcgaagatgc gcacggtgcg cccgcttccg gcgtcgcgaa
     7321 tgacataggc atgccacttc gctttgtgcg tccagcccgg attggccgat ccagtcaaga
     7381 aaagaggggc ccctgttgag gacaagcccg ggctggcggc cagtatttgt ccgttcgaac
     7441 cggtcacttc gaacagcagg cgcatcgata ttttgtcgtc atcgtcatca cgtggcttga
     7501 ggctcgctct ggcaaatgcc gacagatccc gcgcatcgag cacgagcaac gcgcgggcgg
     7561 tctgctcaat gcgggtttcg tcccactccc ccacttcgtc gaccgcctga tggtagctgg
     7621 aatacagcgc gtacgcccag accagtgcga ctccggataa catcaggaag gtcagacgtt
     7681 tcctgatcga gcccctcacg acatgttctc catcacgtag ccaacgccgc gaatcgtgcg
     7741 gatcagcttg gcgcccagtt tcttgcgcaa attcgagaca tgcacctcga tcgcgttgct
     7801 ctcgatctct tcattccagc catagaggct atcctgaaga cgcgcgcggg aaagcggatg
     7861 acccatgttc gccaggagat cgaccaataa tgcgcattca cgtgaggtga gtgcgatacg
     7921 ctcgtttccc cgcatgacca tcaggcgctc tgggtcgacc agcagatcac cgtattcgat
     7981 cgtttccgtg ccgcgcccct gtgcgcgtcg cacgagagcg cgacaacgcg cgatcaattc
     8041 cgtgaggtcg aatggcttgc cgagatagtc gtcggcgccg gcgctcagcc cgctcacacg
     8101 gtcggcaacc gtatcgcgcg cagtcatgac aagcaccggt gtgtgatccc cgcgtttccg
     8161 gatccatccg agcagttcgg ttcccgacat tcgtggcaac cccagatcaa gcacgaccag
     8221 ggcgtacggc gtggtgtcca gggcgagttg cgcctgcctg ccgtcgcggg cccaatcgac
     8281 ggacatgcca gcctcggcca gaccatcctc gaccccgctg cctatcaggt tgtcatcctc
     8341 gacaagtaat acccgcatgg tgccgcgctc ccgcccgttg ccgccaaagt ccccgctgat
     8401 cgtcaataac gataaccgct tagacatacg cgcatcaagc acgcccgttg acgagatcgg
     8461 ctcttaagtt tcgcttaatc ttgagagcct aaactcgcgg gcattgaaat ttcatatcgg
     8521 gtgtccgtcc atgcataaat ctccattccg cctccttttc cccgttctga tgtgtgcctc
     8581 ttttctggcc gccgcgccgc ttgcgagggc cggcgagatc aagactctga tgagggacat
     8641 gaagcatgcc atgcggggag cgctggccag tcaaaccatg cctgatctga gccgccatgt
     8701 ggcgcggttg gaaagcgatg cgcatcgggc aagccgtcaa ctctatcgaa gcgatcagtc
     8761 gacctacgac aaaggcatgc gcgcgttgcg acatgagctc accgaggtcg atcaagccat
     8821 tcaagcgaac aatatgaaag ccgccaaacg cgcgttgcgc aagatcaacg tcacaaagag
     8881 gcattaccac catctgctgg gatgacggat gtgttcaatc gaacagaggt gacccccatg
     8941 aaacaggtaa tccgctaccc tctttcgatc agcttactgc attggttgct ggccatcgcg
     9001 ctgatcggca acctggtcat cggttggctg ctcgacgaca acgaaggcct gctggatctg
     9061 cataagtcga tcggcatcgc cattctcggg ctggtactgc tccgcctggt gaacaggttg
     9121 cgtgtgcgac accaactgcc accctcaacc aacgcggctg gcacgtcagc ctatcgcgtt
     9181 gaaaagcttg ttcatcgctt gctctacgcg ctcatgctgg ccatcccgtt gctggggtgg
     9241 ctcaagacca atgcggctgg gcatgcggcc aactgtttcg ggctgttctc gctgccgacg
     9301 ttgatgccaa ggagccatac gttgtcgccc tggcttgggc aactgcattc actggccgcc
     9361 tacagtttgg ccgcgctggt tggcttgcac gtgctggggg cgctggctca ttggatgttc
     9421 aaagcggaaa atatctttcc tcgtattctc cccttacccg gacgtcttgc acggcagaag
     9481 acatcgctgg gtgatcgagg tcgagcgagc caggatgctt gagagggctg atggtcatgc
     9541 tctctgtcat cctgaccatg gccacgatgt ttcctgatgt ttctggtaca acaccaaccg
     9601 ctttgccgac cagcgctacg acgatatggg cttgtcgctt gcgaatccgg ccggcgcgtg
     9661 attccccgcc tggcaccgcg gcgaccatga ttactgatac cttgattgtc tgggaacaat
     9721 caatcgccct gatcgtcatc gtcgccatgg cgccacttgt ggccgcgcca gcggggacgg
     9781 tctcgccagt ggtcgtcatg atggtcgtaa cggggatacg cccagccata gtaaggggta
     9841 tagacgggcc tgggtgggac gaccaccacg ggaggaggcg gcggcgccac gtactccaca
     9901 ggagggtagt acgccggtgg ggcaaccacc acgccaggca cacccagacc gaaccccggg
     9961 atgccaatcc caataccaat gtcgacatgg gccagcgcgg gtgtcgagca tgtcaccgct
    10021 aatgccatgg cagcggccaa taggctccga ttcatgaaat tcacctgtcg ttgatcgaat
    10081 ggctgcattg tgacgcgact gcctgtttct ggcctgaaat cctggc
//
LOCUS       DASTNK010000041         8346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_769009, whole genome shotgun sequence.
ACCESSION   DASTNK010000041 DASTNK010000000
VERSION     DASTNK010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8346
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769009"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /locus_tag="VFR20_03430"
     CDS             complement(<1..1464)
                     /locus_tag="VFR20_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0229599.1"
                     /translation="MSNTPDENNHADASGGTATHCITRRRLIQATGAAGLAAIAAPLF
                     PFSTAYAAERTLKLLQWSHFVPTYDKWFDQFAKDWGEKNNVKVTVDHISIADLVTTTS
                     SEISANSGHDLIELGSEAAAFSPSVLDMADINQEVAKACGPEFYASTRVSYNPVAKEW
                     YSFCHGWTIDCGDYRQSLWTKAGMPYGPDTWNDILVTGADIMQKQGIPVGIGMSQELD
                     SNMVARAVLWAWDTYIQDEWDNVVLDQGVFKRRAVEAVKYMSDLFHKAMTPEIFAWNA
                     ASNNQALIAGKASYILNSISAYRSAQEKKPDIAKDVFFTGPLAGPNGTRWGNVHVLYN
                     YIIPKFAKAREDTAKQFIKHLVENYDQAMYHSKLYNSPSFFDTAVPKGNRGYPAVKDA
                     ARLLDLNNAWFADDPFRLQGEATGKLKPLLGATNWTTNLGHPGYSNPAAGEVFNTFVL
                     PNMMAKAARGSLSPEAAVEDAARQCRRIYESWRKRGLI"
     gene            complement(1699..3174)
                     /locus_tag="VFR20_03435"
     CDS             complement(1699..3174)
                     /locus_tag="VFR20_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU0229600.1"
                     /translation="MHTLNHTAPDDETALRDLFDATPTALWLEDYSLLHDLFDSWRKQ
                     GVTDLPSFLKQDSTRLAACAASMRIVRVNRRTLSMYAAASSEELVTRLGEILRDALAE
                     AYISELDQLWRGNTSFQSQTVNYALDGRRLDILLKGVVLPDNESTWDRVLVSIDDITE
                     LENARRQLVASEQYAQGIFEQAPVSLWVEDFSAIRHLLDSVREQGIMDFRTFTDVHPE
                     FVTRCMSEIRVLNVNRYTLYMFNGANKHDLLGRLPEVFREEMLPHFREQLIDLWDGQF
                     FQQREVVNHTLDGTQLNIHMQFSVLPGHEKDWSLVLLALTDITARKKAENYLEYLGTH
                     DVLTKLKNRSFYVDEIKRLERKGPFPVTVIIIDLNNLKDINDTQGHTVGDAVLRRAGE
                     VLQKASDKNIHAARIGGDEFALLMPDTDEIKGAVMLDSIRKLVDLNNQFYTGPTLSLS
                     MGIASCRQGSELSEALRRADLAMYDDKRAFYGSSDQNRRRQ"
     gene            3334..3681
                     /locus_tag="VFR20_03440"
     CDS             3334..3681
                     /locus_tag="VFR20_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229601.1"
                     /translation="MESRISQAAGRLILVATAVCGLAGCVAYVPPQTTTYSYYDSTGA
                     LVHVRTETAQQVSEPIYVAPAPAYVAPAYVAPAYVGPPVYLGFGFDFFSGGWGHRHHG
                     WGGRYRGWHGRRW"
     gene            3803..4468
                     /locus_tag="VFR20_03445"
     CDS             3803..4468
                     /locus_tag="VFR20_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEU0229602.1"
                     /translation="MSVAQLLWNFPSAVMQWAVTVRGVAHVGVRMAFDAALFVKALRK
                     SPSRVGAVLPSSSALANAITSELDSSGGPVLELGVGTGVFTRKMLEIGVPPEDLILVE
                     LDSRLADNLRYRFPASHVLCADASRLYRSAVALEVRSVVCGLPLRNMSVKCKLGILRS
                     VFEALDADGAMYLFSYGLRCPIEPRLLDRLGLRARRTGIVYRNAPPARVWKISRRARM
                     FKR"
     gene            4784..5740
                     /locus_tag="VFR20_03450"
     CDS             4784..5740
                     /locus_tag="VFR20_03450"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU0229603.1"
                     /translation="MGGPDVLGDDSPAEARFVDVHRRLLGGLDGGQLLIALYDDQDRL
                     HYANQLFRTTFLQGMEGELDFESIVRFNHARGTGVRIRDLSVDDYLVHVQQRRRSQPR
                     RAFPVELIDGRRFWLTETLLDDGWLLCEACDITAVKRAEMSLRISRDMALEASQTDFL
                     TKLPNRRYGYTFLEHMLTFARTEAVCISVALIDLDYFKLVNDRFGHEAGDLALCRFAD
                     MLRTNMRASEDLAARIGGEEFLVIWPNAGGEIAVESLMRLRARPLEVELPGGANHFAI
                     TFSAGVAQARPDDTVQSLLDRADRMLYAVKTQGRNDVKMDNE"
     gene            complement(5770..6162)
                     /locus_tag="VFR20_03455"
     CDS             complement(5770..6162)
                     /locus_tag="VFR20_03455"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU0229604.1"
                     /translation="MTTLPIDNPFLEWLGVELTAWSEGYAEMRLAMTPNLSNRSARAH
                     GGVLCTLLDSVAGYAGLYAPPGAPRLSGLTLSLTTNFLHSVDGNFLIAKGYVERRGRS
                     IYFSRAEVWQDDTQLLATAMGSFKYRHA"
     gene            6297..6497
                     /locus_tag="VFR20_03460"
     CDS             6297..6497
                     /locus_tag="VFR20_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HEU0229605.1"
                     /translation="MAIEDRLIDIELKLTAQEDLTQSLSQQVYRQQKLIDELRALCGM
                     LLRRLDDAGGSDPYAQEKPPHY"
     gene            complement(6603..7466)
                     /locus_tag="VFR20_03465"
     CDS             complement(6603..7466)
                     /locus_tag="VFR20_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0229606.1"
                     /translation="MSNAQNTVTFHDGRTAPQVGFGVWQIENDKTPAAVAAAFNAGYR
                     LVDTAAMYHNEQGVGVAVAQSDIGREQLFITTKLWNDRHGHDQTIAAFDESLAKLGLD
                     YVDLYLIHWPVPKQKLYVDAWEALIRLRDEGRAKSIGVCNFSTSHLQTLLDKTGVIPV
                     VNQIELHPNFQQTELRDFHAEHGIITQAWSPLGRGRLWDDPVLGALAHKHNRSVAQIM
                     IRWHLQLGNMVIPKSATPGRILENFQVFDFQLDDDDMRAIEALDTPDGRHGPDPEEFS
                     LGTEAHQAAGR"
     gene            7796..>8346
                     /locus_tag="VFR20_03470"
     CDS             7796..>8346
                     /locus_tag="VFR20_03470"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEU0229607.1"
                     /translation="MSVHTQATRVTIPQIMAYKSQRKIAALTSYTAPFARLLDECLDL
                     ILVGDSTAMVGYGLPNTLSITADQMAAHAAAVVRSTSHACILVDMPFGSYQESASSAF
                     ANAARMLAASGADGIKLEGGAALAPTTRFLVDRGVPVLAHVGLMPQYKNTMGGFKAQG
                     MTDESAERILQDALAHEEAGAFAV"
BASE COUNT         1454 a         2310 c         2749 g         1833 t
ORIGIN      
        1 gatcaggccg cgcttgcgcc agctctcgta gatgcggcgg cactgccgtg ccgcgtcttc
       61 gacggcggct tccggcgaga ggctgccacg tgccgccttg gccatcatgt tgggcaacac
      121 gaaggtgttg aagacttcgc cggcggccgg gttggaatag ccggggtgcc ccaggttcgt
      181 ggtccagttg gtggcgccca gcagcggttt cagcttgccc gtggcttcgc cttgaaggcg
      241 gaacggatcg tcggcgaacc aggcgttgtt cagatccagc aggcgggcgg cgtctttcac
      301 ggccggataa ccgcgattcc ctttggggac ggccgtgtcg aaaaagctgg gcgagttgta
      361 gagtttgctg tggtacatgg cctggtcgta gttttcgacc aggtgcttga tgaactgttt
      421 ggcggtgtct tcgcgggcct tggcgaattt cgggatgatg tagttgtata gcacgtggac
      481 gttgccccag cgtgtcccgt tggggccggc cagcgggccg gtgaagaaaa catctttggc
      541 gatgtcgggc tttttctcct gggccgagcg ataggctgag atggagttga ggatgtatga
      601 cgctttaccc gcgatcagcg cctgattgtt ggacgccgca ttccacgcga agatctcggg
      661 ggtcatggct ttgtggaaca ggtcggacat gtatttgacg gcttcgacgg cacgccgttt
      721 gaacacgccc tggtccagca cgacgttgtc ccattcgtcc tgaatgtacg tgtcccaggc
      781 ccacaggacg gcgcgcgcca ccatgttgga gtccagttcc tgcgacatgc cgatgccgac
      841 cggtatgcct tgtttttgca tgatgtccgc gccagtgacc agaatgtcgt tccaggtgtc
      901 tggcccatag ggcatgcccg ccttcgtcca gagcgattgg cggtaatcgc cgcagtctat
      961 ggtccagccg tggcaaaagc tgtaccactc tttggccacg gggttgtatg agacccgtgt
     1021 ggacgcatag aactccggcc cgcaggcttt ggcgacttcc tgattgatgt cggccatgtc
     1081 cagcacgctg ggcgagaacg ccgcggcctc ggacccgagt tcgatcaggt cgtgcccgga
     1141 attggcggat atctcggagg aggtggtggt gaccaggtcg gcgatgctga tgtggtcgac
     1201 agtgactttg acgttgttct tttcgcccca gtctttggcg aattgatcga accatttgtc
     1261 gtaggtgggt acgaaatggc tccattgcag cagtttgagc gtgcgctcag cggcgtaggc
     1321 ggtgctgaat ggaaacagcg gggcggcgat ggcggccagc ccggcggcgc cggtggcttg
     1381 tatcaggcgc cgtcgggtga tgcagtgcgt ggcggtgccg ccagatgcgt cggcgtgatt
     1441 gttttcgtca ggggtgtttg acatgaccgg cctccttggt ggggatgagg gggcctcatg
     1501 gcggtgcgcg aagccatccc tcggttagga atgcagcggc gtagcggtcg taggtatcag
     1561 gcgcgtcacg cgaagcaaaa aaatcgcatg cagaatgtgt aaaacatagt aagtcaaaga
     1621 aacagcgatg tctatattca ggcgatacgc cattcgggcg atatgtcgcc gggtggtgcg
     1681 cgatggggat gatgcgccct attgtcgacg gcggttctgg tcgctcgatc cgtagaacgc
     1741 gcgcttgtcg tcgtacatgg ccaagtcggc gcggcgcaag gcttcgctga gctcgctgcc
     1801 ttggcgacaa ctggcgatcc ccatggaaag gctcaaggtg gggccggtgt agaactggtt
     1861 gttcaaatcg accagcttgc ggatgctgtc gagcatgaca gcgcccttga tctcatcggt
     1921 atcgggcatg agcagggcga attcatcacc gcctattcgt gcggcatgga tgttcttgtc
     1981 cgacgccttt tgcaggacct cccctgcccg ccgcagaacg gcgtcgccca cggtgtggcc
     2041 ctgggtatcg ttgatgtctt tcaggttatt gagatcgatg atgatgactg tgacggggaa
     2101 cgggcctttg cgctcgaggc gtttgatctc gtcgacgtag aacgaccggt tcttgagctt
     2161 ggtcagcacg tcgtgtgtgc ctaggtactc caggtagttc tccgctttct tgcgcgccgt
     2221 gatatccgtc aaggccagca gcaccaggct ccagtctttt tcgtgcccgg gcaggacaga
     2281 gaactgcatg tggatgttca actgggtacc gtccagcgtg tggttgacga cttcacgttg
     2341 ctggaaaaac tgaccatccc atagatcaat cagttgttcg cggaagtgcg gcagcatttc
     2401 ttcgcggaat acttccggaa ggcgtcccag caagtcgtgc ttgttggcgc cattgaacat
     2461 gtagagggtg tagcggttga cgttcagcac ccgtatttcc gacatgcagc gcgtcacgaa
     2521 ttccggatgc acgtcggtga acgtgcggaa atccatgatg ccttgttccc gcacgctgtc
     2581 gagcagatgg cggatggcgc tgaagtcttc cacccacaac gaaacgggcg cttgttcgaa
     2641 gatgccttgg gcgtattgtt cgcttgccac caattgacgg cgggcgttct caagttccgt
     2701 gatgtcgtcg atcgacacca gcacccgatc ccaggttgat tcgttgtccg gcagcaccac
     2761 gcccttcagc aggatgtcca gccggcgtcc atccagggcg tagttcacgg tctggctctg
     2821 gaacgaggta ttgccgcgcc ataattgatc cagctcggag atgtatgcct cggccagggc
     2881 gtcgcgcagg atttcaccca ggcgcgtgac gagttcctcg gatgaggcgg cggcgtacat
     2941 ggacagggtg cgacggttga cacgcacgat ccgcatgctc gccgcacagg cggcaaggcg
     3001 ggtggagtcc tgcttcagga acgaaggcag gtcggtgaca ccctgtttcc gccaggagtc
     3061 gaacagatca tgcaacaaac tgtagtcttc aagccagagc gccgtcgggg tggcgtcaaa
     3121 gagatctcgc agagcggttt cgtcatctgg agcggtatgg ttcagggtgt gcatgagcga
     3181 atggccggtg agggggcaaa ttgttgtttc agcgtgttgt ggtggtgccc cagcatgatt
     3241 gtaccggcgg cataatggtg aagagtggat gctgtcgggc gttcgcgatg cgacgccgaa
     3301 gcactatacg acttttcttt ggggtggtga atcatggaat cccgaatttc ccaagcggcg
     3361 gggcgcctga tactcgtcgc gaccgctgtc tgtggcctgg ccggctgcgt ggcctatgtg
     3421 cccccgcaga cgacgaccta ttcgtattac gacagcacag gtgccctggt gcatgtgcgg
     3481 accgaaaccg cgcagcaggt gtcggaaccc atttacgtgg cgccggcccc cgcctatgtg
     3541 gcccccgcct acgtggcccc cgcctatgtc ggcccaccgg tctatctggg ctttggtttc
     3601 gatttcttca gtggcggatg ggggcatcgc catcatggct ggggcggccg ctatcgcgga
     3661 tggcatggcc ggcggtggta gggcgcgggc accgggcggt ttttttaggg gatggagcgg
     3721 ctcttcccgt tggcgctatg atttatcctg gctccaagga tcgacgcatt ggtcggtgcc
     3781 gaagcccagg aggggagaat ctatgtctgt cgcccagttg ctgtggaact ttccttcggc
     3841 cgtgatgcag tgggccgtca ctgtgcgtgg tgtcgcgcac gtgggtgttc gaatggcttt
     3901 cgacgccgcg ctgttcgtca aggcgctgcg caaaagcccg tcgagagtcg gcgcggtact
     3961 gccctccagt tcagccctag ccaacgcgat caccagcgaa ctggactctt ctggcggccc
     4021 ggtgctcgaa ctcggtgtcg gtacaggggt tttcacacgg aaaatgctgg aaatcggtgt
     4081 gccgcccgaa gacctgattc tggtcgagct ggattcgcgc cttgccgaca atctgcgcta
     4141 tcggttcccg gcctcgcacg tgttgtgcgc cgacgcgtcg cgcctgtatc gttccgcagt
     4201 ggcactggag gtccgatcgg tggtgtgcgg gctgcctttg cgaaacatgt ccgtcaaatg
     4261 caagttgggc atcctgcgca gcgtcttcga ggcactcgac gccgacgggg ccatgtatct
     4321 tttctcgtac ggcctgcgtt gcccgatcga gcctcgtctc ctggatcgtc tggggttgcg
     4381 tgcgcgccgc acgggcattg tgtatcgcaa cgcgccacct gcgcgggtgt ggaagatctc
     4441 tcggcgggca cgaatgttca agcgataaac gcctgcgaaa aggcgagtgg gcatggcggt
     4501 ttccggttcc cgcaagatga acactgatga gttttcgttg ggttttattt catattgtta
     4561 aattttgaca agtactaggg cgttgtacac gccttagtat ttattttcat tacactaaat
     4621 gtgtatcgga tctggtcatt ggggcgcgtt gcggtgtcaa ggccaggatt cgagggagtc
     4681 cgcgctccgt tctcttggtt tcgaatttcg gatcgccgtg gcggcgggcc tgccgcgatt
     4741 ttcccgctta cacaacaaaa attcgaacca gtgatgagga cccatgggcg gaccggacgt
     4801 tttgggcgac gatagccctg ccgaggcgcg gtttgtggat gtgcataggc gtctgctggg
     4861 cggcttggac ggtggacagc ttctgatcgc gctgtacgat gatcaggata ggttgcacta
     4921 cgccaatcag ttgttccgca cgacatttct ccagggcatg gagggcgagc tcgacttcga
     4981 gagtattgtt cgattcaatc atgcgcgggg caccggcgtc cggatccggg acctgtccgt
     5041 cgatgactat ctggtgcatg ttcagcaacg acggcgctcg cagccacggc gcgcgtttcc
     5101 cgtcgagctg atcgatggcc gtcggttttg gcttaccgaa accttgctgg atgacggctg
     5161 gctgttgtgc gaggcctgcg acatcacagc cgtcaagcgg gccgaaatgt cgctgcgcat
     5221 ctcccgggac atggcgctgg aggcttcaca aacggacttc ctcaccaagt tgccgaaccg
     5281 gcgttacggc tacacgttcc tggaacatat gctgactttc gcgcgcaccg aagctgtgtg
     5341 cataagtgtc gcgttgatcg atctggatta tttcaagctg gtgaatgacc gcttcggcca
     5401 tgaagccggc gatctcgcgt tgtgtcgctt cgccgatatg ttgcgaacca acatgcgggc
     5461 gtctgaggat ctggcggccc gtattggagg cgaggaattc ctggttatct ggcccaacgc
     5521 gggcggggag atcgccgttg agtcactgat gcgcctgcgc gcgcgcccac tcgaggttga
     5581 acttcctggc ggggctaatc acttcgccat cacattctct gccggggtgg cccaggcaag
     5641 acccgacgac accgtgcagt ctctgctgga ccgggcggac cggatgctct atgccgtcaa
     5701 gacgcaaggg cgcaacgacg tgaaaatgga taatgaatag gtcatttggc ggcacctgat
     5761 cagcgggcct caggcgtgac ggtatttgaa cgaccccatc gcggtggcga gcaactgcgt
     5821 atcgtcttgc cagacctcgg ctcttgagaa atagatactg cggccgcgac gctcgacgta
     5881 gcccttggcg atcaggaaat tcccgtccac gctatgcagg aagttcgtcg tcagtgacag
     5941 cgtgaggcca ctgaggcgtg gcgcgccggg gggcgcgtag agtcccgcgt accccgcgac
     6001 ggaatccaga agcgtgcaca gcacgccgcc gtgcgcgcgc gcggagcggt tgcttagatt
     6061 cggtgtcatg gccagccgca tttcggcata gccttcggac caggcggtca attcgacgcc
     6121 gagccattcc aggaatgggt tgtcaatggg aagcgtggtc atgtgcgata ggtggtgttt
     6181 ccaatgaacc ctatcgtaca ggaaagccgc gccgaggctt gtcagggcgg atcatggcgg
     6241 ggtgcttcga cgagccccgc gttacactgc gggcttgttc acctgggaag cacgccgtgg
     6301 ccatcgaaga tcgcctgata gacattgaac tgaagctcac agcccaggaa gacctcacgc
     6361 agtccctgag ccagcaggtg tatcgacagc agaagttgat cgacgaattg cgggccttgt
     6421 gcgggatgct gctgcgccgc ctggatgacg cgggcgggtc cgatccctac gcgcaagaaa
     6481 agccgccgca ttattaaaaa gacggttctc cggacgagcc acggttctcc ggatgaaccg
     6541 cggcgcacgc atccgtggat tgaggtcgtc cgttgcgtcg cgaatccccg cgatggcgac
     6601 ggttatcgcc ccgccgcctg atgcgcctcg gtgccgaggc tgaattcctc ggggtcgggg
     6661 ccatggcggc cgtcgggcgt gtccaacgcc tcgatggcgc gcatgtcatc gtcgtccagc
     6721 tggaagtcga acacctggaa attttccagg atgcgccccg gagtggccga tttcggaatc
     6781 accatgttgc ccaactgaag atgccagcgg atcatgatct gggccacgga gcggttgtgc
     6841 ttgtgggcga gggcgcccag gacggggtcg tcccacaggc gtcctcggcc cagcgggctc
     6901 caggcttgcg tgatgatgcc gtgttccgcg tggaaatcgc gtagctcggt ttgttggaag
     6961 ttcggatgca gttcgatctg gttgaccacg ggaatgaccc cggttttgtc cagcagcgtt
     7021 tgcaagtggc tggtgctgaa gttgcacacg cctatggact tggcccgccc ctcgtcacgc
     7081 aggcgtatca gcgcttccca ggcatcgacg tagagcttct gcttgggcac gggccagtgt
     7141 atgaggtaga ggtccacata gtccagaccg agcttggcca ggctttcgtc gaatgcggcg
     7201 atggtctgat cgtggccatg gcgatcattc caaagcttgg tggtgatgaa aagttgttcc
     7261 cggcctatgt cgctttgcgc cacggccacc cccacgcctt gttcgttgtg atacatcgcg
     7321 gcggtgtcca ccaggcggta gccggcattg aaggccgccg cgacagcggc gggcgttttg
     7381 tcgttctcga tctgccatac gccgaagcct acttgcggcg ccgtgcggcc atcgtgaaaa
     7441 gtgacagtgt tctgtgcgtt tgacatgaag tgcctccggg aaggggaaag ccggttccgt
     7501 tggccgcggg ccccgtgggt agaggttgcg atggatagtg cgccaggggc ggcgtggggt
     7561 tgttgccgat gtgctgtacg attgttcgcg catggatgcg cgccgccgcc tgatcggaat
     7621 gggaaccggt gcgttgttgt tttcggtcgg gccattatac ggacgccgag tgggcgacgc
     7681 atcttttgtg tcttgcattt cgtttcagtg tttcatgtgg cgggtccgcc tgcgatgagt
     7741 gttcggacca accaagcgag gcactggcat cacccagggt attcaggaga aagtcatgag
     7801 cgtacacaca caggcaactc gcgtcaccat cccccagatc atggcataca agagtcagag
     7861 gaagatcgcg gccctcacgt cgtacacggc gccgttcgcc aggttgctgg acgaatgcct
     7921 cgatctgata ctcgtgggtg actccaccgc gatggtgggg tatggcctgc ccaacacgct
     7981 gtccatcacg gccgatcaga tggcggcgca cgcggccgct gtggtgcgct ccaccagcca
     8041 tgcctgcatc ctcgtcgaca tgccgttcgg cagttatcag gaatcggcct cgagcgcgtt
     8101 cgccaatgcc gcgcgcatgc tcgcggccag tggcgccgac ggcatcaagc tcgagggcgg
     8161 cgccgcgttg gcgccgacca cgcgtttctt ggtggatcgt ggcgtgccgg tgctggcgca
     8221 cgttgggttg atgccgcagt acaagaacac catgggtggg ttcaaggccc agggcatgac
     8281 agacgaatcg gccgagcgca ttttgcagga cgcgcttgcc cacgaagagg ccggcgcttt
     8341 cgccgt
//
LOCUS       DASTNK010000042         3057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_214711, whole genome shotgun sequence.
ACCESSION   DASTNK010000042 DASTNK010000000
VERSION     DASTNK010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214711"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(449..1360)
                     /gene="gcvA"
                     /locus_tag="VFR20_03475"
     CDS             complement(449..1360)
                     /gene="gcvA"
                     /locus_tag="VFR20_03475"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HEU0229608.1"
                     /translation="MPDSLPPLRALRAFEATARHASVTLAAEELHVTPGAVSVQIREL
                     ESHLGVSLFMRLPRRLVLTSAGERYFPVIREAFRTVRAATADVIAQSQRQVITIACTP
                     SIAVQWLVPRLGALEQQMPGIDIRVSPSTRVMDFSRDAIDLAIRHSSGRHDGLVSEKL
                     LDDKIMPACSPALLDRLGPIGQPDDLRRFPLLHCEDRREWREWLDAAGATLVDASHGP
                     LFINSNGEIEAAKAGLGVVLVRLSLIEKELAEGVLVVPLPQGLVSDMAYYLVYPPMTL
                     DRPTARAVRDWLVGEAKAAASKVIGCD"
     gene            1542..2477
                     /locus_tag="VFR20_03480"
     CDS             1542..2477
                     /locus_tag="VFR20_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0229609.1"
                     /translation="MVQKPMGLTSWGMLLGLSVLWGGSFFFMEVAVRELPTFTFVVLR
                     VGLAAAVLLTLARLMGLALPRGRTVWAAFFCMGVLNNLVPFCLIAWGETRIAGGLASI
                     LNATTPLFAVVVAHMLTDDEKISVNKIIGVVVGFVGVAVMIGPTLFDGMGGHVWAQVA
                     VLGAALSYSFAGIFGKRFKKMGVPPMLTAAGQLSASTVMLLPVALLVDRPWTIAMPDL
                     HVWGAVLGIALLSTALAYVVYFRALAAMGATNVMLVTFLIPVTAILLSWLVLGEHLAA
                     RDFVGMAFIALGLAAIDGRLVNAWRREQCAEVPSR"
     gene            complement(2602..>3057)
                     /locus_tag="VFR20_03485"
     CDS             complement(2602..>3057)
                     /locus_tag="VFR20_03485"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0229610.1"
                     /translation="VHAEAARLEGTVLPLLERGCTVVVDHFGRPDPTLGVSDPGFQWL
                     LSMAGQPNLFVKLSAPYRNWDQKTEAGRNSARDAARLLLEAYTPARLMWGSDWPHTQF
                     ETSQSYGQSLEDIKALLPDESTRIAVLSETARRFYRFRDSAPEIKKQHS"
BASE COUNT          520 a          833 c          985 g          719 t
ORIGIN      
        1 gcctggtcct ggccgatgtg ggcgatgttg ccggtcagcg gcgcggcgct gccgatcttg
       61 acgacaacgt cggcggaagc ggtttggccg aacgccaggg tgataccggc gaggatcagc
      121 gcggcgcttg tgggtttgag ctgaaacttc atgtgcttga gtcccttgaa ttgggttgct
      181 gtgaatatgg cctgcgaaac ttgccgccga gggtgtttcg gcagtggcga gattctcgca
      241 tgtgttccaa atcgtggtaa gcactctttt cgatttttag tttttgtaaa ccattgaatt
      301 tattgatatt ctcaaaaagc gagggaaaat ccctagaaaa tatcggggga gattttcagc
      361 tcgaactcag tttttgttca gtctgcattc agtattgttt gggtgatccg gtcactgttt
      421 ctcttttttg tttttattta gggacgcatc aatcgcaccc gattaccttg ctggctgcgg
      481 ctttcgcttc acccaccaac cagtcccgga ccgcccgcgc tgttggacga tcaagcgtca
      541 tgggcgggta cacgaggtaa tacgccatgt cgctcaccaa gccttgcggc agcggcacaa
      601 ccagaacgcc ttcggccagt tccttttcta tcagtgacag cctgaccagc accacgccga
      661 ggccggcttt ggcggcctct atttcgccat tgctgttgat gaacaacggg ccgtgcgacg
      721 cgtccacaag ggttgcgccg gcggcatcca gccattcgcg ccattcgcgg cggtcttcgc
      781 agtgcagcag cgggaagcgg cggagatcgt ccggctgtcc gatggggccc agccggtcca
      841 gcaaggcggg gctgcacgcc ggcatgatct tgtcgtcgag cagcttttcg ctgaccaacc
      901 cgtcgtgacg ccccgaactg tggcggatgg ccagatcgat ggcatcgcgc gagaaatcca
      961 tgacccgggt cgacggagag acccggatgt cgatacccgg catctgttgc tccaacgccc
     1021 ccaggcgcgg caccagccat tgcacggcga tgctgggcgt gcaggcgatc gtgatgactt
     1081 gtcgctgcga ttgggcgatc acgtcggcgg tggcggcgcg caccgtccgg aacgcctcac
     1141 ggatcactgg aaaatagcgc tcgccagcgg acgtcagtac caggcggcgt ggaaggcgca
     1201 tgaacagcga tacgccgaga tgggattcca gttcacggat ctgtacgctg accgcgcccg
     1261 gggtgacatg gagttcctcg gcggccagcg tgacgctcgc gtggcgagct gtcgcctcga
     1321 aagccctgag tgctcgcaac gggggtaaag agtctggcat gatttagaaa aattcaacaa
     1381 ttcgtggaaa attattcggt cgtttttcaa ttgtcgtgat tttaaaatct ccgtatacca
     1441 gccgtttttt cgaggtatcg gtatggcgcc agccggaagg cgggctcctg tgccggctgg
     1501 gatgcgggtt tagtttaatc aaacttttga agagacacat catggttcag aagccgatgg
     1561 gactgacgtc ctggggcatg ttgctggggt tgtccgtctt atggggcggg tcgttcttct
     1621 tcatggaggt ggcggtcagg gaattgccca ctttcacgtt cgtggttttg cgtgtcggcc
     1681 tggctgcggc cgttctgctc acgttggcgc gtctcatggg cctggcgctg ccccgcgggc
     1741 gcacagtctg ggcggcgttc ttctgcatgg gggtgttgaa caatctggtg ccgttctgcc
     1801 tgatcgcgtg gggtgagacg cggatagccg gcgggctggc gtccattctg aacgcgacca
     1861 ccccgttgtt cgcggtcgtt gtggcgcaca tgctcaccga cgacgaaaag atttccgtca
     1921 acaagatcat cggtgtggtc gtggggtttg tgggtgtggc ggtcatgatc ggcccgacat
     1981 tgttcgacgg catgggtggg cacgtgtggg cgcaggttgc ggtgcttggc gcggcactgt
     2041 cgtattcgtt cgccgggatt ttcgggaaac gcttcaagaa gatgggtgtg ccacccatgc
     2101 tgacggccgc ggggcagctc tcggcctcga cggtgatgct gctacccgtg gcattgttgg
     2161 tcgaccgtcc ctggacgatc gccatgccgg atcttcatgt gtggggcgcg gtgctgggca
     2221 tcgcgctgct gtccacggcg ttggcctatg tcgtgtattt ccgggcgttg gccgccatgg
     2281 gcgcgaccaa tgtcatgctg gtgaccttcc tcattcccgt caccgccatt ctcttgagct
     2341 ggctcgttct gggggaacat cttgcggcac gggatttcgt gggcatggcc tttatcgccc
     2401 tggggcttgc ggcgatagac gggcggctgg tgaatgcatg gcgccgggaa caatgcgcgg
     2461 aagtgcccag ccgctgactg gttgaaattc atccatggca gagcgcgtgc acggattttc
     2521 tgccttgcgc gaaagtgtcc ccgggttctt cacggggtta tgcacaggat gtgtggataa
     2581 ccggtccgga gcctgttttc tttaactgtg ctgttttttg atctcggggg ctgaatcgcg
     2641 gaacctgtag aatctgcgcg ccgtttcaga caggacagcg attcgtgtgg actcgtcggg
     2701 aagcaatgcc ttgatgtctt cgagcgattg gccgtaggat tgcgaggttt caaattgcgt
     2761 atgcggccaa tcgcttcccc acatcaatcg cgctggcgta taggcctcga gcagcaatcg
     2821 tgccgcgtca cgggcgctgt tgcgaccagc ttctgttttc tggtcccagt ttcggtatgg
     2881 tgccgacaat ttcacgaaca gattcggctg gcccgccatg ctcagcaacc actgaaagcc
     2941 tggatcgctg acgccgaggg ttgggtcggg cctgccgaaa tgatcgacga cgaccgtgca
     3001 accccgctcc agcagcggca agacagtgcc ctcgaggcgg gccgcctccg catgtac
//
LOCUS       DASTNK010000043         4988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_215887, whole genome shotgun sequence.
ACCESSION   DASTNK010000043 DASTNK010000000
VERSION     DASTNK010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215887"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..830)
                     /locus_tag="VFR20_03490"
     CDS             complement(129..830)
                     /locus_tag="VFR20_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0229611.1"
                     /translation="MMLQVRDIHSYYGKSHVIQGVSLEVRDGELVTLLGRNGAGKSTT
                     LKSIAGLVTPANGQVLFEGRDITRLAPHRIAGQGLCLVPEHRGIFRLLTVEENLNLGR
                     KKHSPWSLQDIYRIFPRLRERRRNGGAQLSGGEQQMLAIGRALMNAPRLLMLDEPVEG
                     LAPVIVEEIVDQLHQIKSQGIAILLVEQNLQVCTQLAERHYIIESGQIVYTGDNAAFM
                     QDTHVHDQYLGVGTD"
     gene            complement(827..1747)
                     /locus_tag="VFR20_03495"
     CDS             complement(827..1747)
                     /locus_tag="VFR20_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0229612.1"
                     /translation="MNTSARPSEGRVPSHGEDVAKRQEGGDVHTPARPPEGRVPSHGE
                     DGAKRQEGGDIHPLLEARHIVKRYGRFLALAGVDLRVREHTIHSVIGPNGAGKTTLFH
                     SLTGTVPLTEGTILFDGHDVSHQPDHKRVQIGMARSFQVTSLFPTLSVSENLRLAAQG
                     PRQLRALRLWSPIRHEEHAALVEAILQKTGLSSVRNNLSSALSHGQQRRLEVGMALAA
                     RPRAIFLDEPTSGMGIDDIGSMKKLIHGLRDELTVILIEHNMGIVMDISDTITVMQQG
                     QVLVEGAPQAIKADERVKRAYLGNMITGGR"
     gene            complement(1744..2721)
                     /locus_tag="VFR20_03500"
     CDS             complement(1744..2721)
                     /locus_tag="VFR20_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0229613.1"
                     /translation="MMAALRRHQSLLLALVTVLVLPLILKSGSLATEVLIYALATLAC
                     NVLLGYTGLLSFGQGIFFGVGSYTLGILLTRTTLPMPVVLLAAIVVGALVAAAVGWLS
                     IRQKGVYFVMLTLALAQMFYFLAYSLPDWTGGDNGLLNIPAPPLTVFGHTLLPNHTSW
                     QYYGFVAVLFLIVFYLLQRVVDSIFGRTLLAIRDNPARASAIGYDITLFKLVAFMVSG
                     ATTALAGALYAMATGIAPLTSINYHMSETILIMTVIGGTGSLFASVLGAALYVIGSDW
                     LSNLWPRWLLVFGVLMMVVSLYLQGGLWGLCRLVFGRLTPTRAARKEGA"
     gene            complement(2718..3575)
                     /locus_tag="VFR20_03505"
     CDS             complement(2718..3575)
                     /locus_tag="VFR20_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0229614.1"
                     /translation="MNVYLLQVINGIGIGMIYFLLAVGLSIIFGLLRFVNFAHGAFYV
                     LGAYACYQALQWGANFWWTLLIGPCVVGGFAWLIERLVLRNVYNLPHEFHILFTVGLA
                     LAIQEIVILVWGPVGVNVSPPDLLKGVVMWGSFIYPKYRLFVIAITAVCAVLLWLLLE
                     RTRLGSIVRAGSESPEMVTLLSINVHRIFGLVFALGAATAGLAGTLAAPLRGVEPFMG
                     VEAIAIAFVVVVIGGMGSFPGALLGGLIVGIVQSLMSTLWPPGANLMIYVSMALILLL
                     RPNGLLGRA"
     gene            complement(3625..4833)
                     /locus_tag="VFR20_03510"
     CDS             complement(3625..4833)
                     /locus_tag="VFR20_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0229615.1"
                     /translation="MNRRELLKLGALASLSPALAGIRPAFAADTGPILFGCPNPMSGP
                     FAANGKYAAMGMQLIIEETGKALGRPLEYKILDSQGKPAVAIRQVQDLFQQSNAHFFA
                     GGILSSSALAMGKALDKLGGIFVTTAGADEITGKDCNHATFRWSVPTYGAVQETVGPL
                     IKMLPKAKRWYTITPKYVFGEGLLTATKNLLKKAGLEHVGNSYHGLDEREFSGYLTNA
                     MATKPDVLVLLNFGSQSSETLRQAVNFGMKSKMTILIAWASGLEQFESLGADICEDVY
                     FGVQYWHQIDSPLNHTFVKQTQAKFKINPNYSLAGTYICTKLMLDGINKAGSAEPAKV
                     IAAMEGAKYSGMTGPEEIRAADHQVIKNYYLLKGKAKSKMKDKDDYCDILHAGQSFLS
                     PAEAGCKMGT"
BASE COUNT          923 a         1496 c         1650 g          919 t
ORIGIN      
        1 ctcgggtcga aaatttcggg acgggcggcc cgttgtgcga gccaccaggg tagggccggc
       61 tcaagtgtcg tatcgtggat gtgtcgattt cattatgcac tcgaattgat acgccagagg
      121 gcacggggct agtcagtgcc cacgcccaga tactggtcgt gaacgtgggt gtcttgcatg
      181 aacgcggcat tgtcgccggt gtagacgatc tggccgcttt caataatgta atgacgttcg
      241 gccagttgcg tgcacacctg caggttctgc tcgaccagca ggatggcgat gccttgcgac
      301 ttgatctggt gaagctggtc gacgatctct tccacgatga ccggcgccag gccttcgacg
      361 ggttcgtcca gcatcagcag ccgtggcgcg ttcatgagcg cccggccaat ggccagcatc
      421 tgctgctcgc cccccgacaa ctgcgcgcca ccgttgcgtc ggcgttcgcg caggcgcggg
      481 aagatgcggt agatgtcctg caatgaccag ggcgagtgtt tcttgcggcc caggttgagg
      541 ttttcctcga cggtgagcag gcggaaaatg ccgcggtgct ccggcaccag acacaagcct
      601 tgccccgcga tgcggtgggg cgccaggcgc gtgatgtcgc gcccctcgaa cagcacctgc
      661 ccgttggctg gcgtgaccag tccggcgatg ctcttgagcg tcgtggactt gcccgcgccg
      721 ttgcggccca gcagcgtgac gagttctccg tcccgcactt ccaggctcac gccctggatg
      781 acatggctct tgccgtagta gctgtggatg tcgcgcacct gcagcatcag cggcctccgg
      841 tgatcatgtt gcccagatag gcgcgcttga cgcgctcgtc ggccttgatg gcctgcggag
      901 cgccttccac cagcacttgg ccctgctgca tgacagtgat ggtgtcggag atgtccatga
      961 cgatgcccat gttgtgctcg atcaggatga cagtgagctc gtcgcgcagc ccgtggatga
     1021 gttttttcat ggagccgata tcgtcgatac ccattcccga ggtgggctcg tccaggaaga
     1081 tggcccgggg gcgcgcggcc agcgccatgc ccacttcgag tcggcgctgc tggccgtgtg
     1141 acagcgcgct gctcaggttg ttgcgcaccg acgataggcc ggtcttttgc aggatggcct
     1201 cgaccagtgc ggcgtgttcc tcgtgccgaa taggcgacca cagacgcaac gcccgcaact
     1261 gcctgggccc ctgcgccgcg aggcgcaggt tttcgctgac ggacaaggtg ggaaagagcg
     1321 acgtcacctg gaacgagcgc gccatgccga tctgcacgcg cttgtggtcg ggttggtggc
     1381 tgacatcgtg tccgtcgaag aggatggtgc cctcggtcag cgggacggtg cctgtgagcg
     1441 agtgaaacag ggtggtcttg ccggccccgt tggggccgat gacggaatga atggtgtgct
     1501 cacggacgcg cagatccacc cccgccagcg ccaggaacct gccgtagcgc ttgacgatgt
     1561 ggcgggcttc cagcaggggg tgaatgtcac caccctcttg ccgcttcgcg ccatcctccc
     1621 cgtgggaggg aacacgccct tcgggcggcc gcgcgggcgt gtgaacgtca ccgccctcct
     1681 gtcgcttcgc gacatcctcc ccgtgggagg gaacacgtcc ctcggacggc cgtgcggacg
     1741 tgttcatgcc ccctcctttc gcgccgcgcg ggtgggggtg aggcggccga acacgaggcg
     1801 gcacaggccc cacagtccgc cctgcaggta caggctgacc accatcatca gtacgccgaa
     1861 caccagcagc cagcgcggcc acaggttgga cagccagtcg gatccgatga cgtacagggc
     1921 ggcgcccagg accgaggcga acaggctgcc cgtgccgcct atgacggtca tgatcagaat
     1981 ggtttcgctc atgtgatagt tgatgctggt gagcggagcg atgcctgtcg ccatggcata
     2041 gagcgcgccc gccagcgccg tggtcgcgcc cgataccatg aaggccacga gtttgaacag
     2101 ggtgatgtcg tagccgatgg ccgaggcgcg cgcggggttg tcgcggatgg ccagcagggt
     2161 acgcccgaag atcgaatcca ccacgcgctg caacagataa aacacaatca ggaacaggac
     2221 ggcgacgaat ccgtaatatt gccagcttgt gtgattgggc agcagcgtat ggccgaagac
     2281 ggtgagcggc ggagcgggga tgttcagcag gccgttgtcg ccgcccgtcc agtccggcaa
     2341 cgagtacgcc aggaaataga acatctgcgc gagcgccaga gtcagcatga cgaagtacac
     2401 gcctttctgc cggatgctca gccaccccac tgccgccgcc accagcgcgc ccacgacgat
     2461 ggccgcgagc agcaccaccg gcatgggcag cgtggtgcgg gtcagcagga tgcccagggt
     2521 gtagctgccg acgccgaaga agatgccttg cccgaacgac agcagccccg tgtagcccag
     2581 caagacattg caggccagcg tggccagggc gtagatgagc acttcggtgg ccagcgatcc
     2641 cgatttcagg atgaggggca gcaccagcac cgtgacaagg gccagcagca gggattgatg
     2701 tctgcgcaac gcggccatca tgccctcccc agcagcccat tcgggcgaag cagcagaatc
     2761 agcgccatgg acacgtagat catcaggttg gcgcccggtg gccacaaggt gctcatgagg
     2821 ctttgcacga tgccgactat caacccgccc agcagtgcac cggggaaact gcccatgccg
     2881 cctatgacca cgacgacgaa ggcgatggcg atggcttcca cgcccatgaa aggttccacg
     2941 ccgcgcagtg gggcggccag ggtgccggcc aggccggccg tggccgcccc cagggcgaac
     3001 accaggccga agatgcgatg cacgttgatg ctgagcagcg tgaccatttc aggcgactcg
     3061 ctgccggcgc gcacgatgct gcccagtcgg gtgcgttcca gcagcagcca cagcagcacg
     3121 gcgcaaacgg ccgtgatggc gatgacgaac agacggtact tggggtagat gaagctgccc
     3181 cacatcacca cgcctttgag taaatcgggc ggtgacacgt tcacgccaac cggcccccag
     3241 accaggatga cgatttcctg gatggccagc gccagcccca cggtgaagag tatgtggaac
     3301 tcgtgcggca ggttatatac attgcgcagc acgagtcgtt cgatcagcca ggcgaagccg
     3361 ccgaccacgc acgggccgat cagcaaggtc caccagaagt tggcgcccca ctgcaaggcc
     3421 tgatagcagg cgtaggcccc cagcacgtaa aaggcgccgt gcgcgaaatt cacgaagcgc
     3481 agcaacccga aaatgatgga caggccgacg gcgagcagga aataaatcat cccgatgccg
     3541 atgccgttga tcacttgcag cagatagacg ttcataggct ggcgtatgcg tcgggagcgg
     3601 gggctggcgc cccgcgggaa ggcgtcaggt acccatcttg cagccggctt cggccggaga
     3661 caggaaagac tggccggcgt gcagaatgtc gcagtagtcg tccttgtctt tcattttgct
     3721 cttggctttg cccttgagca ggtaatagtt cttgatgacc tggtggtcgg ccgcgcggat
     3781 ttcctcgggc ccggtcatgc cgctgtattt ggcgccttcc atggcggcga tcaccttggc
     3841 gggttcggcc gaccccgcct tgttgatgcc gtcgagcatg agcttggtgc aaatataggt
     3901 acccgccagg ctgtagttgg ggttgatctt gaacttggcc tgagtctgct tgacgaaggt
     3961 gtggttcagt gggctgtcga tctggtgcca gtactgcaca ccgaaataca cgtcttcgca
     4021 gatgtcggcg ccaagggatt caaactgttc cagcccggat gcccaggcga tcaggatggt
     4081 catcttggat ttcatgccga aattcaccgc ctggcgcagg gtttcggaac tttgcgaacc
     4141 aaagttcagc agcaccagca cgtccggctt ggtggccatg gcgttggtga ggtagccact
     4201 gaattcgcgc tcgtccagcc cgtggtagct gttgcccacg tgttccagcc ctgctttctt
     4261 cagcaggttc ttggtggcgg tgagcaggcc ctcgccaaac acgtatttgg gagtgatggt
     4321 gtaccagcgc ttggccttgg gcagcatctt gatcagcggg ccgacggttt cctgcacggc
     4381 gccataggtg ggtaccgacc agcggaacgt ggcgtggttg cagtccttgc cggtgatttc
     4441 gtcggcgccg gccgtggtga cgaaaatgcc gcccagcttg tccagagcct tgcccatggc
     4501 cagcgccgac gacgacagga tcccgcctgc gaagaaatgg gcgttcgact gctggaacag
     4561 gtcttgcacc tggcggatgg cgacagcggg tttgccctgc gaatcgagaa tcttgtattc
     4621 caggggccgg cccaacgcct tgccggtttc ttcgatgatc agttgcatgc ccatggcggc
     4681 gtatttgccg ttggcggcga acgggccaga catgggattg gggcagccga acaggatggg
     4741 gccggtgtcg gcggcgaagg cgggccggat accggccagg gcgggtgaca gggaagcaag
     4801 ggcgccgagc ttgagcagtt ctcgtctgtt catggtgttg tctccggaag tgacgaatga
     4861 gcgggtcgtc tgcggccgga aatcctcccc cggtcatgcg gccttgaagc gattctgacg
     4921 gcaaccttat gttgtgtaca tatacacctg atgatagtgt ggccgaccgt atagacgaaa
     4981 aatgcact
//
LOCUS       DASTNK010000044         4622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_216705, whole genome shotgun sequence.
ACCESSION   DASTNK010000044 DASTNK010000000
VERSION     DASTNK010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216705"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..1410
                     /locus_tag="VFR20_03515"
     CDS             367..1410
                     /locus_tag="VFR20_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0229616.1"
                     /translation="MASLSIKNLCTYYDGRKTGRVKAVDGISLEVEDGEFLVLLGASG
                     SGKSTLMRTIAGLEHPSSGEIHIGGQLANDLPPKARNISMVFQSYALYPHKNVAKNIS
                     FPLETSRMPAADIKKKVAWAAELFGIGHLLARKPRELSGGERQRVALARAMVRSPSLF
                     LMDEPLSNLDAKLRHAARREFKRLHGETGVTTIYVTHDQVEAMGLGHRVAVMNFGKIQ
                     QIGTPHEIYHEPGNTFVAQFMGSPPMNLLPDGDRLIGFHPEHCFLTAPGELASDTYLL
                     TVHVQQIEELGADALVYGTVGNDIRTDVIIKLPERESHRVEAGRDCDFHIPTSLLKSF
                     DAKTGQRNDRQGA"
     gene            1412..2350
                     /locus_tag="VFR20_03520"
     CDS             1412..2350
                     /locus_tag="VFR20_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0229617.1"
                     /translation="MAGNTHGARTGLDQQGRDTLLAMGMVLPAILYIVLLLGVPIVLA
                     LYYSLSDVTTGGSTVHFVGLSNFAALLQDPDFIGAVENTLIITIATLIGTLILAVIQA
                     ELLARNFHGKWIVRFLILLPWTAPASLSVIGWLWMLDSTFSPYDWLLRQMGLLGHPGD
                     LFGAFSNMYWLGRAGLATFSIILVNIWRMLPLATVIILAGLNGMPRDIYEQAQIDGAG
                     YFRRLFQVVFPILYPVFGVSILFTFVFTFTDIVTVYILTRGGPANSTQVLTTLAFFTG
                     VQGGSLGQGAAIALFLLPVLAAVSIVILAFIRRREI"
     gene            2417..3262
                     /locus_tag="VFR20_03525"
     CDS             2417..3262
                     /locus_tag="VFR20_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU0229618.1"
                     /translation="MTRTTKRLGLRGGLYVGICLFTLFSALPFYVMLITSFKSQADIF
                     SPANDPFWFTDAPTLANIQLLFLHTQFVRWLLNTAFVGLSVVVITLILSIPAAYALAR
                     IAGGWGETLGIGIFLSYLVPPTLLFIPFSKVIAFLGLQNSLWSLVLAYPTFTIPFCTW
                     LLMGFFKGIPTDIEEQAMIDGQGRLGAFIHVVLPLAIPGILTVVVFAFTLSTSEFIYA
                     LAFVTNSTQKTISIAVPTDLIRGDVYHWGPLLAGALIASAPVAVLYTFFIDQLVAGLT
                     AVAKD"
     gene            complement(3275..3676)
                     /locus_tag="VFR20_03530"
     CDS             complement(3275..3676)
                     /locus_tag="VFR20_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU0229619.1"
                     /translation="MSSDDLGKLVLRLVLGILLLLHGVSKLTHGISGIEGMVVAHGLP
                     AFLGSAVYIGEVVAPILMIIGMYTRLAGVLVVINMLVALTLVHGGHFFGLGSSGGWRL
                     ELQGFYLFCGLAVALLGAGGYSVKGRSGKWN"
     gene            3879..>4622
                     /locus_tag="VFR20_03535"
     CDS             3879..>4622
                     /locus_tag="VFR20_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEU0229620.1"
                     /translation="MVSSPDNISMAVFCDFENVALGVREANHEKFDIRPVLERLLLKG
                     SIAVKKAYCDWERYKGFKATMHEANFELIEIPHVRQSGKNSADIRLVVDALDLCYTKA
                     HVNTFVIISGDSDFSPLVSKLRENAKQVIGVGVKHSTSDLLVANCDEFIFYDDLIKSR
                     GATRGRQAGKGAAPHDTPVATGADDTHRAEKPQSLKDAAIAMAVETFDALMAERGDSG
                     KIWASTLKQAMKRRKPDFSESNHGFRTFGN"
BASE COUNT          893 a         1523 c         1340 g          866 t
ORIGIN      
        1 ggcgaagtct tcaacacctt cgtgttgccc aacatgatgg ccaaggcggc acgtggcagc
       61 ctctcgccgg aagccgccgt cgaagacgcg gcacggcagt gccgccgcat ctacgagagc
      121 tggcgcaagc gcggcctgat cggaggcaag gcctgattct gtagttcgcg cgggcgggca
      181 cttgtcatcc gcccgcgcta tcgcggggat ttgcgccttg ccgtgcgggc gaatccatcg
      241 atgtcatcat ggccccgaac tgccgggaca gcacactaaa ggcgggcgcc atgtcccaac
      301 ccggctctct ggccaggggg gcgcaacgtg caggcttgcc tcgacaagcc taattggagg
      361 actgccttgg caagcctgag catcaagaat ctttgcacgt actacgacgg gcgtaagacc
      421 ggccgcgtga aagccgtcga cggcatcagt ctcgaggtcg aggacggcga gttcctggtc
      481 ctgctgggcg cctcgggttc gggcaaatcg accctcatgc ggacaatcgc cgggttggaa
      541 caccccagca gcggcgagat ccacataggc gggcaactcg ccaatgatct accgcccaag
      601 gcgcgcaaca tctccatggt gttccagagc tacgccctgt acccgcacaa gaacgtggcg
      661 aagaacattt cgttcccgct cgaaacctcc agaatgcccg cggccgacat caagaagaaa
      721 gtcgcctggg ccgccgaact gttcggcatt ggccacctgc tcgcccgcaa gccacgcgaa
      781 ctatccggcg gcgagcgcca gcgggtggcc ctggcaaggg ccatggtgcg ttcgcccagc
      841 ctgttcctga tggacgagcc actgtccaac ctggacgcca agctgcgcca cgccgcaagg
      901 cgcgaattca aacggttgca tggcgaaacc ggcgtcacca ccatttacgt cacgcatgac
      961 caggtggagg ccatgggcct gggccaccgc gtggctgtca tgaattttgg aaagatccag
     1021 cagatcggca ccccgcacga gatctatcac gagcccggca acaccttcgt ggcccagttc
     1081 atgggttccc cccccatgaa cctgttgccc gacggcgacc ggctcatagg cttccacccc
     1141 gaacactgct tcctgacggc gccgggcgaa ttggcgtcgg acacctatct tctcacagtg
     1201 catgttcaac aaatagagga actgggtgcc gacgcgctgg tctatggcac tgtcggcaac
     1261 gacatccgca ccgacgtcat catcaagctg ccggaacggg aatcgcaccg cgtcgaggcg
     1321 ggccgggatt gcgacttcca tatccccaca tccctgctca aaagcttcga tgcgaaaacc
     1381 ggtcagcgca acgaccgcca aggcgcctga catggcgggc aacacacacg gcgcccgcac
     1441 cgggctggat caacaaggcc gcgacacgct gctggcgatg ggcatggtgc tgcccgctat
     1501 cctgtatatc gtgctgctgc tgggcgtgcc catcgtattg gcgctctatt acagcctgag
     1561 cgacgtcacc acgggcggat cgaccgtgca cttcgtgggg ttgagcaatt tcgcggcact
     1621 tcttcaggat cccgacttca tcggcgcggt ggagaacacg ctgatcatca ccatcgcgac
     1681 gctgatcggc accctcatcc tggccgtcat acaggccgaa ctgctggcgc gcaacttcca
     1741 tggcaaatgg atcgtgcgtt ttctcattct gctgccctgg accgcgccgg cctcgctcag
     1801 cgtgattggc tggctgtgga tgctggattc cacgttcagc ccctacgact ggctgctgcg
     1861 ccagatgggg ctgctgggcc atcccggcga cctgttcggc gcattctcca acatgtactg
     1921 gctgggccgg gcgggcctgg ccacgttttc catcattctg gtcaacatct ggcgcatgct
     1981 gccgctggcc accgtcatta ttctggccgg tctcaacggc atgccacgcg acatctacga
     2041 acaggcgcag atcgacggcg ccggctactt ccgccgtctg ttccaggtgg tgttccccat
     2101 cctgtatccc gtcttcggcg tgagcatcct gttcacattc gtcttcacgt tcaccgacat
     2161 cgtgacggtc tacatcctga cccgcggtgg gccggcgaac agcacccagg tgctgacgac
     2221 gctggccttt ttcaccgggg tgcagggggg ttcgttgggg cagggcgcgg ccatcgcctt
     2281 gttcctgcta cctgtcctgg ccgccgtgtc catcgtcatc ctggcgttca tacgccgccg
     2341 ggagatctag cgtgcgcccg gacacccctt ttcgtgacgt cgcaccagaa tccgcgcgag
     2401 cggaaaggat cgccacatga cgcggacaac gaaacgactg ggattgcgcg gcggcttgta
     2461 cgtgggcatc tgcctgttca cactgttttc ggccttgccg ttttacgtga tgctgatcac
     2521 gtcgttcaag agccaggccg acatcttctc gcccgccaat gacccattct ggttcaccga
     2581 cgcgcccacg ctcgccaaca tccagttgct gttcctgcac acgcagttcg tgcgctggct
     2641 gctcaacacg gcgttcgtgg gcctgtccgt cgtcgtcatc accttgatcc tgtccattcc
     2701 cgccgcgtat gcgctcgcgc gcatcgccgg cggttggggc gaaaccctgg gcatcggcat
     2761 tttcctcagc tatctcgtgc cgcccacgct gttgttcatc ccgttttcaa aggtcatcgc
     2821 cttcctgggg ttgcaaaact cgctgtggtc actagtactg gcctatccca ccttcaccat
     2881 tccgttctgc acctggctgc tgatgggttt cttcaagggc atccccaccg acatcgagga
     2941 acaggccatg atagacggcc aggggcgtct gggcgccttc atccatgtgg tgctgcccct
     3001 ggccattccg gggatactca ccgtcgtcgt cttcgccttc acactgtcga ccagcgaatt
     3061 catctatgcc ctggccttcg tgaccaactc gacgcagaag accatcagca tcgcggtgcc
     3121 caccgacctg atacgcggcg acgtctacca ctgggggccg ttgctggccg gcgcgctgat
     3181 cgccagcgcg cccgtggccg tgctgtatac gttcttcatc gaccagttgg tggcggggtt
     3241 gacggccgtg gcgaaggatt gagcgccccc cgcatcaatt ccacttcccg cttcgcccct
     3301 tcacgctgta gccgcccgcc cccaacaagg ccacagccaa gccgcagaac aggtagaacc
     3361 cttgcagctc caggcgccag ccacccgaag agccaagccc gaagaagtgc ccaccatgca
     3421 ccagggtgag agccaccagc atgttgatga ccacgagcac gccggccagc cgggtgtaca
     3481 tgccgatgat catcaagatg ggggccacga cttcaccgat gtagaccgcc gaccccagga
     3541 atgccggcag gccgtgggcc acgaccatgc cctctatgcc cgagatgccg tgcgtgagct
     3601 tggacacacc atgcaacaac agaagaatgc ccagcaccag gcgcaaaacc agctttccca
     3661 gatcgtcgga agacatgcct aaaccctttc catgaaaaaa acaggggtat tctacaagag
     3721 cgcccagtat cctggattag agatttgttg aaaaacaaat cactctggat cggcgccagg
     3781 caaggcgcaa accacagcgt agcctgcacc agccggttgt atccggcacc gcgccgacgc
     3841 ggtactatcg acccaccttt tcctccagga cgacacacat ggtttccagc cccgacaaca
     3901 tcagcatggc cgtcttctgc gatttcgaga atgtcgcgct gggcgtgcgc gaggccaacc
     3961 acgagaaatt cgatatccgc ccggtgttgg aacgcctgct gctgaagggc agcatcgcgg
     4021 tgaagaaggc gtattgcgac tgggagcggt acaaaggttt caaggccacc atgcacgagg
     4081 cgaatttcga attgattgaa attccccacg tgcgccagtc gggcaagaat tccgcagaca
     4141 tccgcctggt ggtcgacgcg ctggacctgt gctacaccaa ggcgcatgtc aacaccttcg
     4201 tcatcatcag tggcgattcc gacttttccc cgctggtgtc caagctgcgc gagaacgcga
     4261 aacaagtcat tggcgtcggg gtcaagcatt ccacctcgga cttgctggtg gccaactgcg
     4321 acgagttcat cttctacgac gatctgatca agagtcgtgg cgccacgcgg ggccgccagg
     4381 cgggcaaggg cgcggcgccg cacgacacgc ccgtggcaac gggggccgac gacacgcatc
     4441 gcgccgagaa accccagtcc ctgaaggacg cggccattgc gatggcggtg gaaacgttcg
     4501 acgcactgat ggccgagcgc ggcgacagcg gaaagatctg ggcgtcaacg ttgaagcagg
     4561 ccatgaagcg gcgcaagcct gatttcagcg agtccaacca tggcttccgc accttcggaa
     4621 at
//
LOCUS       DASTNK010000045        11186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1514535, whole genome shotgun sequence.
ACCESSION   DASTNK010000045 DASTNK010000000
VERSION     DASTNK010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11186
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514535"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /gene="rnpB"
                     /locus_tag="VFR20_03540"
     ncRNA           <1..379
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFR20_03540"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            711..1139
                     /gene="mraZ"
                     /locus_tag="VFR20_03545"
     CDS             711..1139
                     /gene="mraZ"
                     /locus_tag="VFR20_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEU0229621.1"
                     /translation="MFQGSSALTLDAKGRISIPTRHRDALVAQVEGQLTLTRHPDGCL
                     LLYPRSVWEIKREQIVAFPASARPLQRLLLGSAQDVELDGSGRILISPELRTAVGMTR
                     EVMLLGMGSHFELWDATEWSRREAEDLAKGMPQALDQFSF"
     gene            1148..2221
                     /gene="rsmH"
                     /locus_tag="VFR20_03550"
     CDS             1148..2221
                     /gene="rsmH"
                     /locus_tag="VFR20_03550"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU0229622.1"
                     /translation="MEFVHRSVLLEPTVNALLDPAFGLRSTRKRSDRSAADSPPDRSH
                     GIFVDGTFGRGGHSRLLLSRLSDDARLVVFDKDPVAIESARALERADSRVHVVHGGFA
                     SMVEALTGLGIDRIDGVMLDLGISSPQVDDAQRGFSFMREGPLDMRMDTSSGLTAAQW
                     LAEADVEEIQEVIENYGEERFAFQIAKAVVDRRQSRPLRTTLDLAELVASVVRTREKG
                     QHPATRTFQALRIHLNQELEELALALKAILKLLACGGRLAVISFHSLEDRMVKQCIAA
                     AARPAAAQARLPIPEKDLPPPILISLGKVMPDEQEVRDNARARSAVLRVAQRTDAPLP
                     ADAGESFVARPALRRRAAQRRRR"
     gene            2221..2526
                     /gene="ftsL"
                     /locus_tag="VFR20_03555"
     CDS             2221..2526
                     /gene="ftsL"
                     /locus_tag="VFR20_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEU0229623.1"
                     /translation="MGRLCILFAVLLMTSAMSLVTARFKSRELFVVLDRLSTQAHALD
                     NDWRRLQLARAELARNARIDSIARTDLGMVSAPPDKTIYIDGGSPVVTAAEQAGGQP"
     gene            2523..4268
                     /locus_tag="VFR20_03560"
     CDS             2523..4268
                     /locus_tag="VFR20_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU0229624.1"
                     /translation="MKRVRFHDSPVLNVQIPLWRSRLVLILIMLGFLALSAKALYLQG
                     ISTQFLQQQGERRFERTLVLPATRGKIFDRSGNVVLATSVPAKAIWAIPDDAKDATPE
                     QIAQLGKLLGMSAADIQSRIASDDDKNFVYLKRQVPVSIGEQITQLKVPGIHELAETR
                     RVYPEGDIAAHVLGFTNIEGKGLEGVELAYNSELEGQSGSRRVIRDRLGRIVEDVRAV
                     VPPVNGQDLNLSIDAGIQFDTYTALKQAMEDHKAKSAAAVVIDVQTGEILAMVSLPSY
                     DPNDVEARKGPALRNRAVVDTFEPGSIMKPFTAALALDLGRITTKTIFHTGNGVYRYQ
                     GATIHDVEAGGNGDLNVAGVLRLSSNIGMTMISELLTSKEMWTKFAQLGIGRAPRLHF
                     PGVAPGRLRPWKRWRPIERATMAYGYGLSVSLLQIAHAYTAFARNGDMVSLTLLKRHG
                     APTTVQEYSPRVAGLIRAMLEAAAGPDGAKRAQVQGYRVAGKTGTARQIVDGRYSKSL
                     YRGSFVGFAPVSHPRIVVGVTVDQPTVNGYYGAQVAAPAFSRIVTSCLRRMGVQPDAP
                     MDSLVALGPDKKVSR"
     gene            4265..7054
                     /gene="murF"
                     /locus_tag="VFR20_03565"
     CDS             4265..7054
                     /gene="murF"
                     /locus_tag="VFR20_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase
                     MurE/UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine
                     ligase MurF"
                     /protein_id="HEU0229625.1"
                     /translation="MNARPILAWLDERVERARHLCLDSRRIEPGDVFIACPGQTSDGR
                     LYLRQAADRGAAAIVMQDGWDGARPGIPVLEVEGLARMLGEIADEWYGQPSAAMTVIA
                     ITGTNGKTSTAQWLAAALSAQGIPCGAVGTLGVTRPDGSMSDGALTTPDVLSVHRTLA
                     LLRNAGATAVAIEASSIGLDQGRLDHVRVVVAGFTNLTRDHLDYHGTIEKYREAKFSL
                     FGRSGLQLAVVNVDDAAGRDLLTRYPDLPVLRYSVDAHRESDIRAVDIHTGAYGLIFN
                     LAFPEGTARIVTRQLGLHNVSNLLLVAGVLHHMGWDLARIAAALAALEPVPGRLEIIA
                     PRVSVDARRGAVLVVADYAHTPDALERALTALRPAAAQRGGELVCVFGCGGDRDSGKR
                     PMMGRIAATLADRVVVTNDNPRTEDPRVIAEQIVAGMASAPGIELDRARAILSTVWAV
                     ADQDIVLLAGKGHETYQEFNGGRVPFDDREWAAFALAWRGATHSVSTDSRRIEPGQWF
                     VALQGDNFDGHDYLAQVASAGACAAVVARRDPSVGLPQFMLGDTRAALIHIGAAWRAH
                     HDLPVVGVTGSNGKTTTKEMIAAAVRAWLGGDAVLATQGNMNNDIGVPLMLMRLSGAH
                     RVAVFELGMNHPGEISVLADMARPTVALVNNAQREHQEFMHTVEAVARENGGVINALP
                     DAGVAVYPGDDVFAPLWRELAASRAVVRFGLGTECEVRAEEIHAGVLGTSCKVCTPWG
                     DGILSLPVPGLHNLRNALAAIACAGAVGAPLDAMLRGLAAFTAVRGRMMPRPLGDGLQ
                     LIDDTYNANPDSVRAAIDVLAGLRGRKILVLGDMAEVGQDGPALHAEAGAYANERGID
                     TLLTFGPAAAHASRAFGSGAHAFDSIDALVTHLGSLGRANILVKGSRSTRMERVVQAL
                     EKKMMEKGGGGRHAA"
     gene            7044..8216
                     /gene="mraY"
                     /locus_tag="VFR20_03570"
     CDS             7044..8216
                     /gene="mraY"
                     /locus_tag="VFR20_03570"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU0229626.1"
                     /translation="MLLELARWLSDAHIRAFGVFEYITFRALLACGTSLFIGLLAGPR
                     VIRKLTELKIGQAVRAYGPESHLQKTGTPTMGGVLILISIGVSTLLWADWTNRFVWVV
                     LLVTFGFGWIGWADDYKKVVNRDPEGMRARTKFFWQALIGIVAAVYLAFAVSVPANAD
                     LWPLFRDWVLSGLSMPLPRRADLIVPFFKSVSYPLGVFGFVALTWLVIVGASNAVNLT
                     DGLDGLAIMPTVMVGSALGIFAYVVGRVDYSKYLLFPYIPGASELMVLCAAIAGSGLA
                     FLWFNAYPAQVFMGDVGALALGGALGTIAVIVRQEIVFFIMGGVFVAETLSVMLQVTY
                     FKYTKKRYGQGRRILRMAPLHHHFEVGGWKETQVVVRFWIISMMLVLVGLATLKLR"
     gene            8222..9742
                     /gene="murD"
                     /locus_tag="VFR20_03575"
     CDS             8222..9742
                     /gene="murD"
                     /locus_tag="VFR20_03575"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU0229627.1"
                     /translation="MSFPSVGAGDLTLILGLGETGLAAARWCLTHGARLRVLDTRLKP
                     AGVDALKAEPGAGGIDFRLGPDAVSADALEGAVRVVLSPGLSPLQEPVKSFLARAVER
                     NVEVVGEIELFARALTDMAAHGYAPKVLAVTGTNGKTTVTALTRHLVEAGGLSARAAG
                     NISPAALAALADALASGNLPDVWVLELSSFQLETTTSLVPDAATVLNLTQDHLDWHGS
                     MQAYGAAKARLAAMAKVAIINRDDPLVAAMVPDTRGRDVRTFGVGEPSCCGDMGIENS
                     HGVAWLAASEVNDFDLPVRPSRRKKNEAEPARDEGRMVRLMPLDALRIRGMHNAMNAL
                     AALALARSLGIGWAAMLPALREYRGEPHRTEFVRTIEGVDFINDSKGTNVGATVAALE
                     GLGRKVVLIAGGLGKGQDFSPLSDSVRRHARAVVLIGQDGPIIGRALAQSGVELAHAE
                     SLAEAVKCALGMAREGDAVLLSPACASMDMFRNYRHRGTSFVEEVQALALDRGEMA"
     gene            9739..10932
                     /gene="ftsW"
                     /locus_tag="VFR20_03580"
     CDS             9739..10932
                     /gene="ftsW"
                     /locus_tag="VFR20_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEU0229628.1"
                     /translation="MSLFADLTSSVNAVRPGRTTMRGFDLPLVICASTLVLFGLLMVY
                     SASIGLADGPRYSSYGRFYFVGRHAAFITVGLVCAAITASVPMSVWERLSVPIFLCGM
                     MLLLVVLIPGIGREVNGSRRWLGLGPINFQPSELMKLAVLLFAANYTVRKQQYMKDFV
                     RGFLPMACALAGVGIVLLLEPDLGAFMVCVAIAVGILFIGGINGKLFSTLLSIMVGSF
                     LLLIWLSPWRRERLFVYLDPWNPANTYGSAYQLSHSLIALGRGQWFGVGLGASVEKLH
                     YLPEAHTDFIVSVIGEELGFVGLMGVVCFFAFLVWRGFEIGRQAIAMERIFNGLVAQG
                     VALWFGVQAFINIGVCVGLLPTKGLTLPLVSYGGSSIVMNLVALALLLRVDYENRNLM
                     RGRRT"
     gene            10929..>11186
                     /locus_tag="VFR20_03585"
     CDS             10929..>11186
                     /locus_tag="VFR20_03585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0229629.1"
                     /translation="MNGQTLLIMAGGTGGHIMPGLAVAQEMRRRGWRVLWLGHPERME
                     GRLVPEYGFEMEPLRFSGVRGKGWMALARLPLTLFSACWQAF"
BASE COUNT         1802 a         3278 c         3824 g         2282 t
ORIGIN      
        1 atcgaggaag gtccggactc cacagagcgc gatagcggct aacggccgtc cggcgttgtg
       61 gcgcattgcg tcgccaccaa gccgaggaac agggccacag agacgagtct gcggcgcggg
      121 cgtgcgacag cacgcacagg cacggcgact tccgtgtcgc atgtcttctc acgaagacat
      181 ggcgttgcag ggtgaaacgc ggcaacctct atcgggagca acaccaaata ggcatgcgtg
      241 ggcgcttgcg cccggagagc ggctcgctcg agcatgcggg taggtggcta gagcacggtc
      301 gcaaggccgc gcccagagga atgattgccc gccgggctcg cccggcgtac agaatccggc
      361 ctacaggccc gcctcatttt tccaggctga cagaacgcca tgatcgctct ccgcgaccat
      421 ggcgcgtggt cgagcttatc gagagcgttg acggcgtcgt ttcgtcgccg gacaaccgct
      481 gatacgcccg tttgcaattc ttttgatcct gatgtttctc ttgcagatgg ccggccaatt
      541 tcttgattta cctcgctatt ttattttcac actctccgga aacaggcaac ttcccggttc
      601 taagtgcttg ttgtgattgg aaaaaacaca cttttgcgct tgaccttgcc agaccattcc
      661 cgtagagtgg ggaaaagtag gattttgtgc aataaagtgg ggtaatcgca gtgttccagg
      721 gcagtagtgc actcacgttg gatgccaagg ggcggatctc gattccgacc cggcaccgtg
      781 acgcgctcgt cgcccaggtg gaaggccagc tcacgctgac ccgtcacccc gacggatgcc
      841 tgcttctcta cccccggtca gtctgggaaa taaagcgcga gcagatcgtc gcgttcccgg
      901 cgtcggctcg gccgttgcag cgtctgctgc ttggcagcgc ccaggacgtg gagttggacg
      961 gctccggccg catcctgatc tcgcccgagt tgcgcaccgc cgtcggcatg acgcgtgaag
     1021 tcatgcttct gggaatggga agtcatttcg agctgtggga cgcgacggag tggtcgcgcc
     1081 gcgaagccga agacttggcg aaaggcatgc cccaagcgct ggatcaattt tcattctgac
     1141 gggaactatg gagtttgtgc accgatccgt actgctggag cctacggtca acgccctgct
     1201 tgatccggct tttggcttgc ggtcgacgcg taaacgctcc gatcggtccg cggccgactc
     1261 tccccctgat cgttcacacg ggatcttcgt cgatggcaca ttcggtcgag gtggacacag
     1321 ccggttgttg ttgtcgaggc tgtcggacga tgcgcgtctc gtggtgttcg acaaggatcc
     1381 cgtggccatc gagtcggccc gggcgttgga gcgggccgat tccagagtcc atgtggtgca
     1441 tgggggattc gcctccatgg tcgaggcgct gaccggactg ggtatcgatc ggatcgatgg
     1501 cgtcatgctc gatctcggta tctcatcgcc gcaggtcgac gacgcgcagc ggggattttc
     1561 gttcatgcgg gaaggcccgc tggacatgcg gatggacacc agcagcggtc tgacggccgc
     1621 gcagtggctg gccgaggcag atgtagagga aatacaggag gtcatagaaa attatggcga
     1681 agaacggttt gctttccaga ttgcaaaggc ggttgttgac cgccgccaat cccgcccgct
     1741 gcgcacaacg ctcgacctcg ccgagctcgt cgccagtgtc gtccgcacgc gcgaaaaggg
     1801 tcaacacccg gccacccgca cctttcaggc tttacggatt cacctcaatc aggaactcga
     1861 ggaactcgcg ctcgccctca aggcaattct gaaactgctg gcttgcgggg ggcgcctggc
     1921 cgtgatcagt tttcattctc tcgaagaccg gatggtgaaa cagtgcatcg ccgctgcggc
     1981 gcgccccgcg gcggcacagg cgcgcctgcc cattcccgag aaagacctgc ctccccccat
     2041 cttgatttcc cttggcaagg tgatgcccga cgagcaagag gtccgtgaca acgcgcgtgc
     2101 gcggtcggcc gtgttgcggg tcgcgcagcg caccgacgcg ccactgccgg ccgatgcggg
     2161 ggaatctttc gtcgcgcgtc ccgctctgcg ccgcagggcg gcgcagcgga ggcgccgata
     2221 atggggcgct tgtgcatcct gttcgcggtt ttgctgatga cgtcggccat gtcgctcgtg
     2281 acggcgcggt tcaagtcgcg tgagttgttc gtggtgctgg atcgtctttc gacgcaggcg
     2341 catgcgctcg acaatgactg gcgccggctc cagcttgccc gcgccgagct ggcgcgaaac
     2401 gcgcggatcg acagcatcgc gcgcaccgat ctgggcatgg tgtccgcgcc gcccgacaag
     2461 accatctata ttgatggcgg gtcgccagtg gtgaccgccg ccgagcaagc cggagggcag
     2521 ccatgaagcg cgtccggttc cacgacagcc ccgtgctgaa cgtccagatt ccgttgtggc
     2581 gatcccgtct ggtgctgatt ctgatcatgc tggggttcct ggccttgagc gccaaggcgc
     2641 tgtacctgca aggcatctcc acccagttcc tgcaacagca gggtgaacgt cggttcgagc
     2701 gcacgctggt gttgcccgcg acgcgcggca agatcttcga ccgcagcggc aatgtggtgc
     2761 ttgccaccag cgtgccggcc aaagctatat gggccatccc tgatgacgcc aaagacgcga
     2821 ctcccgaaca gatcgcgcag ctggggaagc tgctgggcat gtccgccgcc gatattcaga
     2881 gccgcatcgc gtcggatgac gacaagaatt tcgtgtacct gaaacgtcag gtgcccgtca
     2941 gcatcggcga gcagatcacg caactgaaag tccccggcat ccacgagctg gccgagacgc
     3001 gccgggtgta tcccgaaggc gacatcgccg cccatgtgct cgggttcacg aatatcgagg
     3061 gcaaggggct ggagggggtt gaactggctt acaattccga actcgaggga cagtccgggt
     3121 cgcgtcgggt cattcgggat cgtctagggc gcatcgtcga ggacgtgcgg gcggttgtgc
     3181 cgcctgtgaa cggccaggac ttgaatctct cgatcgatgc cggaattcag ttcgatacct
     3241 acactgcgct caagcaggcc atggaagacc ataaagccaa gtccgcggcg gccgtcgtga
     3301 tcgacgtcca gaccggtgag atcctggcca tggtcagtct gccgtcgtac gatccgaatg
     3361 atgttgaagc ccgtaaaggc ccggccttgc gcaaccgggc cgtggtcgac acgttcgagc
     3421 caggctccat catgaagcca ttcacagccg ccctcgccct tgacctgggg cggatcacga
     3481 caaaaaccat cttccatacc ggcaacggcg tgtatcgtta tcagggcgcg accatacacg
     3541 acgtcgaagc cggcggcaac ggcgacctga atgtggctgg cgttttgcgt ctatccagca
     3601 atatcggcat gaccatgatt tccgagctgc tgacgtcgaa ggaaatgtgg accaagttcg
     3661 cgcagttggg tataggcagg gcgccgcgat tgcacttccc gggcgtcgcg cccggccgcc
     3721 ttcgtccctg gaagcggtgg cggcccatag agcgggcgac catggcctat ggctatggtc
     3781 tttcggtgtc tctgttgcag atcgcgcatg cctatacggc atttgcccgc aatggcgata
     3841 tggtgtcatt gactttgctc aagcggcatg gcgcgcccac caccgtgcag gaatactcgc
     3901 cacgggtggc tggcctgatc cgtgccatgc tcgaggcggc tgcgggcccg gatggcgcca
     3961 agcgggcgca agtgcaaggc taccgcgtcg cgggcaagac cggcaccgcg cgacagatcg
     4021 tcgacggcag gtatagtaag tcgttgtacc gtggttcctt tgtcggattc gcccctgttt
     4081 cgcaccctcg catcgtcgtc ggtgtcaccg tggatcaacc tacggtcaac ggatattacg
     4141 gggcccaggt ggccgcacca gccttctcgc gcatcgtgac gagttgcctg cgtcgcatgg
     4201 gtgtccagcc ggatgcgccc atggattcct tggtggctct tgggcccgac aagaaggtgt
     4261 cgcgatgaac gcgcgcccga ttctcgcctg gctggacgaa cgcgtcgagc gcgcgcggca
     4321 cttgtgcctg gattcgcgtc gtatcgagcc tggcgatgtg ttcatcgcct gcccgggcca
     4381 gacttcggat ggtcggctct atttgcggca agcggccgat cgtggcgctg cggccatcgt
     4441 catgcaggac gggtgggacg gcgcgcggcc gggaattccc gtgctggaag tcgaggggct
     4501 ggctcggatg ctgggtgaga tcgccgatga atggtacggc caaccctcgg ccgccatgac
     4561 ggtgattgcc atcacgggca cgaatggcaa gacgtcgacc gcgcaatggc tggccgcggc
     4621 gctgagcgcg caaggaatcc cctgtggcgc cgtgggaacc ctgggtgtga cgcggcccga
     4681 cgggtcgatg tccgacgggg cgctgaccac ccctgatgtc ctgtctgtgc atcgcacact
     4741 cgcgctgctg cgtaacgcgg gcgcgacagc ggtggcgatc gaggcctcgt ccatcgggct
     4801 cgaccagggg cgtctggacc acgtgcgcgt ggtggtggcg ggtttcacca atctgacgcg
     4861 ggaccatctg gactatcacg gcacgatcga gaaataccgc gaagccaagt tctcgctgtt
     4921 tggccgatcc gggttgcaac ttgccgtcgt caatgtggac gacgccgccg ggcgcgactt
     4981 gttgacgcga tacccggatc tgccggtgct gcggtactcg gtcgatgcgc accgggagag
     5041 cgatatccgc gccgtggaca tccacaccgg cgcgtacggg ttgattttca atctggcttt
     5101 tccggaagga accgcgcgga tcgtgacccg gcagctaggg ctgcacaatg tgtcgaatct
     5161 cctgctggtg gcgggcgttc ttcaccacat ggggtgggat ctcgcccgca tcgccgccgc
     5221 gttggccgcg cttgaaccgg tgcctggccg tctcgaaatc attgctcccc gtgtgtccgt
     5281 cgatgcgcgg cgcggcgctg tgctggtcgt ggccgactat gcccataccc ccgatgcgct
     5341 cgagcgagcc ctgaccgctc tgcgtccggc cgctgcgcag cgtggcggcg aattggtttg
     5401 cgtgtttggt tgcggcgggg atcgcgacag cggcaagcgc cccatgatgg ggcggatcgc
     5461 cgcgacgctg gccgataggg tggtcgtgac caatgacaac ccccgcaccg aagatccgcg
     5521 agtcattgcc gaacagatcg ttgccggcat ggcgtccgcg cccgggatcg aactcgatcg
     5581 cgcccgggcc attctttcaa cggtctgggc ggtggcggat caggacatcg tgctgctcgc
     5641 cgggaaaggc cacgagacct accaggaatt caatggcggg cgtgtaccct ttgatgatcg
     5701 agaatgggcc gctttcgcgc tggcctggcg gggcgcgacg cattccgtct cgacggattc
     5761 ccgcaggatc gagcccgggc agtggttcgt ggcgctccag ggcgacaact tcgacgggca
     5821 tgattacctt gctcaagtgg cgtctgcggg cgcctgcgcc gcggttgtcg cgcgccgcga
     5881 cccgtcggtc ggcctgccgc aattcatgct gggtgacaca cgcgcggcgt tgattcatat
     5941 cggtgcggcc tggcgcgccc atcacgactt gccggtggtc ggcgtcacgg gaagcaacgg
     6001 caaaaccacc accaaagaaa tgatcgcagc ggccgtgcgc gcctggctgg gcggtgatgc
     6061 cgttctcgcc acgcaaggca acatgaacaa cgacatcgga gtgccgctca tgctcatgcg
     6121 cttgagtggt gcgcatcgcg tggcggtgtt cgaactgggt atgaatcacc cgggcgagat
     6181 cagcgtgctg gcggatatgg cgcggcccac ggtggccctg gtcaacaacg cccaacggga
     6241 gcatcaggaa ttcatgcata cggtggaagc cgtcgcgcgt gagaacggtg gtgtgataaa
     6301 tgcgctgccg gacgctggcg ttgcggtgta tccgggtgac gatgtgtttg cgccgttgtg
     6361 gcgcgagttg gcggcgtcgc gcgccgtggt gcggttcggg cttggcaccg agtgcgaggt
     6421 tcgggccgag gagattcacg cgggtgtgct gggcacgtcg tgcaaagtgt gcactccctg
     6481 gggcgacggc atattgtcgt tgcctgttcc cggcctgcat aacctgcgca acgcgctggc
     6541 cgcgattgcg tgcgcgggcg cggtgggcgc tccgttggat gcgatgctgc gtggactggc
     6601 ggcattcacg gccgtccggg ggcggatgat gccccggccc ctgggtgatg ggctgcaatt
     6661 gatcgacgac acgtacaacg cgaatccgga ttcggtccga gcggcgatcg atgttctggc
     6721 tggtttgcgg ggcaggaaaa tcctggtgtt gggcgatatg gccgaagtcg gtcaggacgg
     6781 cccggcgctg cacgccgaag cgggcgccta cgcgaatgag cggggtatcg atacgctgct
     6841 caccttcggg ccggccgccg cgcatgcgtc ccgagccttc gggtcggggg cccacgcgtt
     6901 cgattcgatc gatgccctgg tgacgcacct tggcagtctt ggacgggcga acattctggt
     6961 gaagggatcc cgcagcacac gcatggagcg tgtggtccag gcgttggaga aaaaaatgat
     7021 ggaaaagggc gggggaggcc gacatgctgc ttgaactggc ccggtggctg tcggatgccc
     7081 acattcgcgc tttcggcgta ttcgaataca tcacgttcag ggctttgttg gcgtgcggca
     7141 cctcgttgtt catcgggctg ctcgcggggc cgcgcgtgat acgtaaactg acggagctga
     7201 agattggtca ggccgtgcgc gcctatggtc cggagtccca tctgcagaaa accggcacgc
     7261 cgaccatggg aggcgttctc atcctgattt ccatcggggt cagcacgttg ttgtgggcgg
     7321 attggaccaa ccggttcgtg tgggtggtgt tgctggtcac cttcgggttt ggctggatcg
     7381 gctgggccga tgactacaag aaggtggtca atcgcgaccc ggagggcatg cgcgcgcgaa
     7441 cgaaattctt ctggcaggcg ctcatcggta tcgtggcggc ggtgtatctg gcgtttgccg
     7501 tgtccgtgcc ggccaatgcc gatctttggc cgctgtttcg cgactgggtc ctgagcgggc
     7561 tgtccatgcc cttgcccagg cgtgccgacc tgatcgtgcc attcttcaag agtgtgagtt
     7621 acccgctggg tgtgttcggt ttcgtcgcgt tgacctggct ggtgatcgtg ggggccagca
     7681 acgcggtgaa tctgaccgac ggccttgatg ggttggccat catgccaacc gtcatggtgg
     7741 ggagcgcgct gggcattttc gcctatgtcg tggggcgtgt ggattattcc aagtatctgc
     7801 tgttccccta tattccgggc gcgtccgagc tcatggtgct gtgtgcggcc atcgcgggtt
     7861 cggggcttgc gttcctttgg ttcaacgcct atccggccca ggtgttcatg ggcgatgtcg
     7921 gcgcgttggc gcttggcgga gcgttgggaa ccatcgccgt gatcgtgcgt caggaaatcg
     7981 tgttcttcat catgggcggg gtgttcgtgg ccgagacgct gtccgtgatg ctgcaggtaa
     8041 cgtacttcaa gtacacgaaa aaacggtatg ggcagggccg gcgcattttg cgcatggcac
     8101 ctctgcacca tcacttcgag gtcggtggtt ggaaggagac ccaggtggtg gtgcgtttct
     8161 ggatcatcag catgatgctg gtacttgtgg gtcttgcgac tcttaaattg cgatgaacgc
     8221 gatgtctttt ccttcggtgg gcgctggcga cctgaccctg atcctggggc tgggggaaac
     8281 cggtttggcg gcggcacgct ggtgtctgac ccatggggcg cggttgcgcg tcctggatac
     8341 gcgcctcaag ccggccggtg tggacgctct gaaagcggag ccaggcgctg gcggcatcga
     8401 tttccgcctt ggccccgacg ccgtgtcggc cgacgccctg gagggggcgg tcagggtggt
     8461 gctcagtccc gggctctctc ccttgcagga gccggtgaaa tcgtttctgg cccgggcggt
     8521 tgaacgaaat gtcgaggtcg tgggggaaat cgaactcttc gcccgcgcgc tgactgacat
     8581 ggcggctcac ggttacgcac ccaaggtgct ggccgtgacg ggcaccaatg gcaagaccac
     8641 cgtcacggcg ttgacgcggc atctggtcga ggctggcggc ttgtccgccc gcgccgccgg
     8701 caatatcagc cccgcggcgc tggcggccct ggccgatgcc ttggcatcgg gaaacctgcc
     8761 ggatgtgtgg gttctcgagc tatcgagctt ccagctcgaa accacgacaa gcctggtgcc
     8821 cgatgccgcg acagtcttga atctgaccca ggaccatctg gattggcatg gctcgatgca
     8881 agcgtacggc gcggcgaagg cgcggttggc ggccatggcg aaagtggcca tcatcaatcg
     8941 cgacgatcca ttggtcgcgg cgatggtgcc tgacacccgg gggcgggatg tgcgtacgtt
     9001 tggcgtgggc gagccgtcgt gttgtggcga catgggcatc gagaacagcc atggcgtggc
     9061 ctggctggcc gcctcggagg tcaacgactt tgatctcccg gttcggccgt cgcggcgcaa
     9121 gaagaatgag gcggagccag cgcgcgatga ggggcgtatg gtgcggttga tgcctcttga
     9181 cgcattgcgc atccggggca tgcataacgc catgaatgcg ctggccgcgc tcgcgttggc
     9241 ccgttcgctg ggtatcggat gggcggcgat gctgccggct ttgcgcgagt atcgcggcga
     9301 gccgcaccgc acggaattcg tgcgaaccat cgagggtgtg gatttcatca acgacagcaa
     9361 gggtaccaat gttggtgcga ccgtcgccgc gctggagggc ctcgggcgga aagtcgtgct
     9421 gatcgccggt ggcctgggca agggccagga cttttcgccc ctgagcgatt cggtgcggcg
     9481 ccatgcgcgc gcggtcgtgc tcatcgggca ggacgggccg atcatcggtc gggcgttggc
     9541 gcagtcggga gtcgaactgg cccatgccga gtcgctggcc gaggccgtga agtgtgccct
     9601 cggcatggcg cgggaagggg acgcggtgtt gctgtcgcct gcgtgcgcca gcatggacat
     9661 gttccgcaat tatcgccacc gtggcacttc gttcgtcgaa gaggtgcaag ccctggctct
     9721 cgacagaggg gagatggcat gagcctgttc gcggacctga catccagcgt caatgccgtt
     9781 cgtcccgggc gcacgacaat gcgcggcttt gaccttccct tggtcatttg cgccagcacg
     9841 ctggtgttgt tcggtttgct catggtgtat tcggcgtcca ttggcctcgc ggatggcccg
     9901 cgctattcga gctatggacg gttctatttc gtgggtcggc atgccgcctt catcaccgtg
     9961 ggcctggttt gcgcggcgat caccgctagc gttcccatga gcgtctggga gcgattgtcc
    10021 gtgccgatct tcctgtgcgg catgatgctg ttgctggttg tgctgatccc cggcatcggc
    10081 cgtgaagtca atggctcccg gcgctggctc ggcctggggc cgatcaactt ccaaccgtcc
    10141 gagctgatga agttggcggt gctgcttttc gcggccaact acacggtgcg aaagcagcaa
    10201 tacatgaagg acttcgtccg ggggttcctg cccatggctt gcgcgctggc aggtgtgggg
    10261 atcgtgctgt tgctcgagcc cgatctgggg gcgttcatgg tgtgcgtcgc catcgccgtg
    10321 ggtatcctgt tcatcggcgg catcaacggc aagcttttct ccacgttgct gagcatcatg
    10381 gtgggcagtt tcctgctgct catctggttg tcgccgtggc ggcgcgagcg tctgtttgtc
    10441 tacctggacc cctggaaccc cgccaacacc tatggcagcg cgtaccagtt gtcgcattcg
    10501 ctgatcgccc ttggccgcgg gcaatggttc ggcgtcgggc tgggcgccag cgtcgagaaa
    10561 ttgcattacc tgcccgaggc acacaccgat ttcatcgtgt cggtcatcgg tgaggaactg
    10621 ggtttcgtcg gtctgatggg ggtcgtgtgt ttcttcgcct tcctggtctg gcgggggttc
    10681 gaaatcggtc gccaggcgat cgccatggag cgcattttca atggtctggt ggctcagggc
    10741 gtggcgttgt ggttcggtgt ccaggctttc atcaacatcg gcgtgtgtgt ggggctgctt
    10801 cccaccaaag ggttgacgct tccgttggtc agctacgggg gttcgagcat cgtcatgaat
    10861 ctggtggccc tcgcgctgtt gctccgggtc gactacgaaa accgcaatct catgcggggg
    10921 cgtcgcacat gaatggccag acgctgttga tcatggcggg tggaacgggc ggacacatca
    10981 tgccggggct ggctgtggcc caggaaatgc gtcggcgcgg ctggcgcgtg ctttggctcg
    11041 gccatcccga gcgcatggaa ggtcggttgg tgcccgaata cggtttcgag atggagcctc
    11101 tgcgtttttc cggtgtgcgc ggcaaaggct ggatggcgtt ggcgcgcttg ccgttgacgc
    11161 tcttcagcgc gtgctggcag gcattc
//
LOCUS       DASTNK010000046         6572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_218128, whole genome shotgun sequence.
ACCESSION   DASTNK010000046 DASTNK010000000
VERSION     DASTNK010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6572
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218128"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="VFR20_03590"
     CDS             <1..1012
                     /locus_tag="VFR20_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0229630.1"
                     /translation="FPVDGAGAWEATTAPADHDTVQNWTINVSGLVQRPKTIKVADLE
                     QMDHVKRISVMQCAGNGRSYYWAKAKTPGSGWKHGGMANLTWEGVPLLPLLKSLELSP
                     ANSVRFLTANGKDVPPVKGGADMIKSYHLAAPQLEQAILAVRMNGKPIPTVHGGPVRL
                     IIPGYYGNMNVKWLTELLLMDEPTPSAIMQKTYRMPIMSVEPGKFTTQDLTRENSTPT
                     YGFAIMSVLFAPLQGQTVKAGDVELKGVAWNDGLASLDDVRVSLDQGKTWMATEIDDS
                     DGPFAWHHWRTQVKLEAGKHEVWVRATDGWGRTQPLNGLTTWNPGGWDWHGIDRVSFE
                     VA"
     gene            1009..1716
                     /locus_tag="VFR20_03595"
     CDS             1009..1716
                     /locus_tag="VFR20_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU0229631.1"
                     /translation="MMAAPIFRRVLLAAALAAASATAAGTEIRLPNGPGANLVYSKCQ
                     ACHDLQYVKDAKGLMAAQWSAVVASMQDYGLKISDQDKATVLKYLTTYLGPNPPPAEA
                     AVPSSAKLDGATLWQQNCASCHGSKGTGQPGYFPPLAGNPDLFKDRMFPVLVVLNGLS
                     GPITVGDQKYNGGMPALGHLSDAEVAVIVNFVRGAWGNEPEAAKLEPITSELVAQERK
                     KTLTPTQVHALRGRFAQ"
     gene            1811..2422
                     /locus_tag="VFR20_03600"
     CDS             1811..2422
                     /locus_tag="VFR20_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEU0229632.1"
                     /translation="MAFHTWLLYFAAAFALSLTPGPNGLLALTNGALYGLRKASWATL
                     GGALGFALLIALSAAGISALLQTSAHVLMALKWLGGAYLIWLGIQVWRSPPPASTLRA
                     TAYVPRGLALFRQGFFSAVSNPKAILFFGAFLTQFIDPQRGLLPQSVIMAVTFVLIEI
                     AMEITIAALAGNIRNGLQRGGKRFNQICGGLFVAIGAYLPWSR"
     gene            complement(2484..3575)
                     /locus_tag="VFR20_03605"
     CDS             complement(2484..3575)
                     /locus_tag="VFR20_03605"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEU0229633.1"
                     /translation="MLNPLFREVDAKLLDAGQLQVGGIHRILVLRPNHRLGNLILITP
                     LLTELEQAFPGAEIDVLTAGGAARDVLAGFPTVRRILALPQYIARHPLSALKTIWSLH
                     GARYDLAVSPATDSYSSRVLVYLAKARNLIGIPPSDSSGRTNWNRILPTAPEHFAKKP
                     VFMLRHALVSTPGADMPPYPPLNVRLTESEQTEGRQALDAVLAERSVAPGRMVIGVFA
                     DASGAKRFDTEWWRLFLNTLAEDHPDYTFMELIPVDGQSRLENRFPSFFSRSIRRFAS
                     LAANLDCVVCADGGIMHLASASGVPTVGLFSVTDPLKYAPYGHNDQAYDTRGKSPEQI
                     ARGVGEIVETIAGARSGYADPSRPGIGAE"
     gene            complement(3807..4013)
                     /locus_tag="VFR20_03610"
     CDS             complement(3807..4013)
                     /locus_tag="VFR20_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229634.1"
                     /translation="MSKSGSIPRKLLKMFIEDRNFALSIIVWVFAYWLVVPSMVASPA
                     GQAVILFAGLGVILADSVLRQARR"
     gene            complement(4010..4783)
                     /locus_tag="VFR20_03615"
     CDS             complement(4010..4783)
                     /locus_tag="VFR20_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229635.1"
                     /translation="MSYLQVDLMHGWPLMAAAFMASLVEFVEALTIVLAVGVARGWRD
                     ALSGSLCAVLVLAGIVALVGPALLSIPMDSVRLLIGLLALLFGMRWLRKAILRTAGVI
                     ALHDEATAYARQSEMLARTNSAPRGGVDLIAFATAFQAVLLEGLEVVFIVIAVGASES
                     RFLPPTVGAGAALVVVVALGAILHRPLLRVPENTLKFAVGVLLCAFGAFWLGEGMGLR
                     WPGEDWSIAGLAAGFLVLALLCVRWARRVSKTLAEGGRP"
     gene            complement(4851..5669)
                     /locus_tag="VFR20_03620"
     CDS             complement(4851..5669)
                     /locus_tag="VFR20_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU0229636.1"
                     /translation="MAAAEIPASADPMVRARDIHKRYGRDEVLKGINVEVAQGEVLCL
                     IGPSGSGKSTFLRCINHLEKIDAGRLWVDGELIGYRERGRYLYEMRERDVSRQRAAIG
                     MVFQRFNLFPHMTALENIIEAPVHVRGEPRARAVEHARELLRHVGLEAKADAYPAMLS
                     GGQQQRVAIARALAMRPKLMLFDEPTSALDPELVDEVLAVMRGLAGEGMTMIVVTHEI
                     AFARDVGDQVAFMDGGVIVESGPPGQVLVNPRHERTRGFLSRVLSQREARSQNG"
     gene            complement(5656..6483)
                     /locus_tag="VFR20_03625"
     CDS             complement(5656..6483)
                     /locus_tag="VFR20_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU0229637.1"
                     /translation="MPSGTLGPTRVVPLRRPGLWLGSTAVVVLLVMLAVSIYRNPNID
                     HAAIVKYQFAPAILTGLRTTIILAVLAAVIGLVLGVVLAVMRLSASWVLRLGSGFYTW
                     LVRGTPLLVQILLWGNLALLFQHIGPFDTNSLMTPFVASVVALGLNEGAYMAEIVRAG
                     ILAVDRGQYEASLSLGMPRTLAMRRIILPQALRVIIPPTGNQFISLLKATSLVSVIAG
                     GDLLTQAENISSANLRTIELMLVATFWYLVLTTITSIGQYFIERRMARSGTSMHGGR"
BASE COUNT         1334 a         2044 c         2011 g         1183 t
ORIGIN      
        1 ttttcccgtc gatggggccg gcgcctggga ggccacgacg gcgccggcag accacgacac
       61 agtccaaaac tggaccatca acgtctcggg cctcgtgcag cggccgaaaa ccatcaaggt
      121 tgccgatctg gaacaaatgg atcacgtgaa gcggatttcg gtcatgcaat gcgccggtaa
      181 tggccgttcc tactactggg ccaaggcgaa aacgccgggt tccgggtgga agcacggcgg
      241 catggcgaat ctcacctggg aaggtgtacc acttctgccc ttgctcaaga gcctcgagct
      301 cagccccgcc aacagcgtca ggttcctgac cgccaatggc aaggacgtcc ctccagtcaa
      361 gggtggcgcc gacatgatca aaagctacca tctggcggca ccgcaactgg agcaggccat
      421 tctcgcggtg cgcatgaatg gcaagccgat tcccaccgtc catggcgggc cggtgcgcct
      481 gatcattccg ggctattacg ggaacatgaa tgtgaaatgg ctcaccgagc tgctgctgat
      541 ggacgagccc acccccagcg cgatcatgca gaaaacgtac cgcatgccaa tcatgtcagt
      601 ggaaccgggc aaattcacca cacaagacct gacccgtgaa aacagcacgc ccacctatgg
      661 tttcgccatc atgagcgtgc tcttcgcgcc gcttcaaggg cagacagtca aggcgggcga
      721 tgtcgaactg aaaggcgtcg cctggaacga cgggctggcc tcgctggacg atgtccgtgt
      781 gtcgctggac caggggaaaa cctggatggc cacggaaatc gatgattccg acggcccgtt
      841 cgcctggcac cattggcgca cacaagtgaa actcgaagcg ggtaagcacg aggtctgggt
      901 gcgcgccacc gacggatggg ggcgcaccca gccgctgaat ggtctcacca cctggaatcc
      961 ggggggctgg gattggcacg gcattgatcg ggtcagcttc gaggtggcat gatggcggcg
     1021 ccgattttca ggcgcgtcct gttggcggct gccctggccg ccgccagcgc cactgccgcg
     1081 ggcaccgaga tccgcctgcc caacgggcca ggcgccaatc ttgtctattc caaatgccag
     1141 gcttgtcacg acttgcagta tgtcaaggac gccaagggtc tgatggccgc acagtggagc
     1201 gcggtcgtgg cgagcatgca ggattatggg ctgaaaatca gcgaccagga caaggccacc
     1261 gtgctcaagt atctgaccac ctatctgggg ccgaatcccc ctccggccga agcggcggtg
     1321 ccctcttccg cgaagctcga tggcgcgacg ctctggcaac agaactgcgc gtcatgccat
     1381 ggctccaagg gcacggggca gcctgggtac tttccgccat tggccggcaa ccccgatctt
     1441 ttcaaggata ggatgttccc ggtgcttgtc gtattgaacg gcctgagcgg ccccataacc
     1501 gtcggggatc agaagtacaa cggtggcatg ccggcgttgg gtcatctttc cgacgccgag
     1561 gttgcggtga ttgtcaattt cgtccgcggc gcttggggga atgaaccgga ggcagcgaag
     1621 cttgaaccga tcacatcgga actggtggcc caggaacgga agaaaaccct gacgcctaca
     1681 caggtacacg ccttgcgcgg gcggtttgcg cagtaaggtg gttggatgtc ggcagcgtca
     1741 gatgcgggtc gactacggta ttatcctgcc gtaggctttc gcgcatctcg tccttcctga
     1801 gtcgatcacc atggcattcc atacttggct gctgtacttc gctgcggctt tcgctttatc
     1861 tctgacgcct ggcccgaatg gcctgctggc gttgaccaat ggcgcgctgt acgggttgcg
     1921 caaagcatcg tgggcgacgt tgggcggcgc cctgggtttc gccctgctga ttgcgctttc
     1981 cgcagcgggg atcagtgcgt tgctgcagac atcggcgcat gtgctcatgg ccttgaaatg
     2041 gctgggtggc gcttacctga tatggctcgg catccaggtc tggcggtcac cgcctcccgc
     2101 ctccacgcta cgcgcgacgg cgtatgtccc acgcggactg gcattgttcc ggcaggggtt
     2161 tttctccgcg gtttccaatc cgaaggccat tctgtttttc ggtgcgttcc tcacgcagtt
     2221 cattgatccg cagcgggggc tcctaccgca atccgtcatc atggccgtga cattcgtctt
     2281 gattgaaatc gccatggaaa taacgatcgc cgctcttgcc gggaacatcc gcaatgggtt
     2341 gcaacgtggt gggaaacgct tcaatcagat ttgtggcggc ctgttcgtgg cgattggcgc
     2401 ctatctgcca tggagccgtt gagtctgcgg caccagtatt ctccccggat accggaagcc
     2461 aagagaaaga gcctctagga gcgctactcc gcaccgatac cgggccgaga agggtccgca
     2521 tacccagatc tggcgcccgc tatcgtttcc acgatctccc ccacaccccg cgcgatttgc
     2581 tcggggctct tcccgcgggt gtcataggcc tggtcgttgt gaccatacgg cgcatacttc
     2641 agcgggtcgg tcacggagaa caacccgacc gtcggcacac cactcgcgct ggcaaggtgc
     2701 atgatgccgc catcggcgca gaccacgcaa tccaggttgg ccgccagcga ggcgaaccgt
     2761 cgaatgcttc gggaaaaaaa ggacgggaat cggttctcca gcctggactg cccatccacg
     2821 ggaatgagtt ccatgaacgt gtagtcagga tgatcttcgg ccagggtgtt caggaaaagc
     2881 cgccaccatt ccgtgtcgaa gcgcttcgcc ccggacgcgt ccgcgaagac gccgatcacc
     2941 atacgccctg gcgcgacaga ccgttctgcc agcacggcat ccaacgcttg ccggccctcg
     3001 gtttgttcgc tctcggtcag gcgcacgttc agcggcgggt acgggggcat gtcggcgcca
     3061 ggtgttgaaa ccagggcatg ccggagcatg aagaccggct tcttcgcgaa gtgctctggc
     3121 gcggtcggca agatacgatt ccagttggtt cgccccgaac tgtcgcttgg tggaatgccg
     3181 atcagattgc gtgctttcgc caaatagaca agcacccggc tcgaatagga gtcggtggcc
     3241 gggctcaccg ccagatcgta ccgggcgccg tgcaatgacc agatggtctt cagggcgctt
     3301 agcggatgac gggctatgta ttgcggtaaa gcaaggatgc ggcgcacggt ggggaaaccg
     3361 gcaagcacgt cacgcgcggc tccaccagcc gtgagcacgt cgatttccgc gcctggaaac
     3421 gcctgttcca gttcggtcag caatggggtg atgaggatga ggtttccgag gcgatggttg
     3481 ggcctcaaga cgagaatgcg gtgaatgcca ccgacttgaa gctgccccgc atccagaagc
     3541 ttggcatcca cttcgcgaaa gagtggatta agcaactggc gccatatccg gcggcgaatt
     3601 tttctgatgt tccctttggc aaaaaaactg ttcggggcat tttgcataga ctggggacct
     3661 tggtccggga aattcggcat cgccatgtta gcgcgaaacc cttgggacag gattagaacg
     3721 aatgtggtgc aattggtttc cggatacgcg gataccagtg cctccgaatg cccaaggaaa
     3781 cgcccctggc tcgctgctgg gtccgttcat cgccgtgcct gacgcagcac actgtcggcc
     3841 agaatcacac ccagcccggc aaacaggatg accgcctgcc ccgccggcga agccaccatg
     3901 gacggaacaa ccagccaata tgcgaatacc cagacgatga tggacagggc gaaattccga
     3961 tcctcgatga acatcttcag gagtttgcgg ggtatcgagc cagacttgct catggccgcc
     4021 ctccttccgc caatgtcttc gataccctgc gcgcccatcg cacacacaat aaggccagga
     4081 ccaggaatcc ggccgccaac ccggcgatcg accagtcttc acccggccag cgcagcccca
     4141 tgccctcgcc tagccagaag gcgccgaacg cacaaagcaa tacgcccacg gcgaacttca
     4201 gcgtgttctc gggaacgcgc agcaatggtc gatgcaggat ggcccccaac gccacgacaa
     4261 ccaccagagc cgcgcccgcc ccgactgtcg gcgggagaaa gcgcgattcg ctcgcaccca
     4321 ctgcgataac gatgaacaca acctcgaggc cctccaggag caccgcctgg aaggctgtgg
     4381 cgaacgcgat gaggtcgact cccccacgtg gcgctgaatt ggtgcgcgcc agcatttcag
     4441 actgtcgcgc ataagcggtt gcctcatcgt gcagggcgat gaccccggcg gtccgcaata
     4501 tggccttgcg cagccagcgc atgccgaaga gcaaagccag cagcccgatg agcaatcgta
     4561 cggaatccat gggtatgcta agcaacgccg gaccaaccag cgccacgatt cccgccaaca
     4621 ccaggacggc gcagagactg ccggacaacg catcgcgcca accccgcgcg acgccgacag
     4681 ccagaacaat ggtcaaggct tcgacgaact caaccaggga tgccatgaac gccgcagcca
     4741 tcaatggcca cccatgcatc aggtcgactt ggagataaga catcgagggg acacccactt
     4801 tccgaaaaag gttgacgggg ggttatatac cgaaacgact taggattgca ttagccattc
     4861 tgagatcgcg cctcgcgctg gctcagtacg cgcgacaaga acccccgggt gcgttcatgc
     4921 cggggattga ccagcacctg ccctggcggg cccgattcga caatcacccc gccatccatg
     4981 aatgcgacct ggtcgccgac gtcccgggca aaagcgatct cgtgcgtgac gacgatcatg
     5041 gtcataccct cccccgccag gccgcgcatg acggccagca cttcgtcgac cagttcgggg
     5101 tcgagcgccg aggtgggctc atcgaacagc atgagtttgg gccgcatggc gagcgccctg
     5161 gcgatcgcca cgcgctgttg ctgcccaccc gacagcatgg cggggtatgc gtcggccttg
     5221 gcctcgagcc ccacatggcg caacagttcc cgggcgtgct cgactgctcg ggcgcgtggt
     5281 tcgccgcgca cgtgcaccgg cgcctcgatg atgttctcca gcgcggtcat gtgcggaaac
     5341 aggttgaagc gctggaacac catgccgatg gcggcgcgtt gccggctgac atcgcgttca
     5401 cgcatttcat acagatagcg gccacgctcg cggtagccaa tgagctcacc gtcgacccac
     5461 agccgcccgg cgtcgatctt ttccaggtga ttgatgcagc gcaggaacgt gcttttgcca
     5521 gagccagaag ggccgatcag gcacaggact tcgccctgcg ccacctcgac gttgatgccc
     5581 ttcaggactt cgtcgcggcc gtaacgcttg tggatatccc gggcgcgcac catggggtcg
     5641 gccgatgcgg gaatttcagc ggccgccatg catgctcgtc ccggaacgcg ccatgcgccg
     5701 ttcaatgaaa tactgaccga tgctggtgat ggtggtcagc accaggtacc agaaggttgc
     5761 gaccagcatc agctcgatgg tccgtaaatt ggcggaggaa atattttcgg cctgggtgag
     5821 gagatcgccg ccagcgatga ccgacaccag cgaggtggct ttgagcaggg aaatgaattg
     5881 gttgcctgtc ggcggaatga tgacccgcaa cgcctgcggc aggatgatgc gccgcatggc
     5941 cagggtgcgc ggcatgccca ggctgagcga ggcctcatac tggccgcgat cgacggccag
     6001 tatgccggcg cgcacgattt ccgccatgta cgccccttca ttcaagccca atgccaccac
     6061 cgacgccacg aacggcgtca tgaggctgtt cgtatcgaaa gggccgatat gctggaacaa
     6121 cagggccaga tttccccaga gcaggatctg taccagcagt ggtgtaccgc gcaccagcca
     6181 ggtgtagaag ccactgccca gccgcagcac ccagcttgcc gacaggcgca tgacggccaa
     6241 caccacgccc agcaccaagc caatgacggc ggctagtact gcgaggatga tggtggtgcg
     6301 caggccggtg agaatggcgg gcgcgaactg gtatttgaca atggccgcgt ggtctatgtt
     6361 tggattgcga tagatcgaca cggccaacat taccagcagc acgaccaccg ccgtgctgcc
     6421 cagccacaac cccggccggc gcaggggcac gacccgcgtg ggcccgagag ttccagatgg
     6481 catggatcag tgcttcagca ggacgtgaat cagagcttca ggttcttgcc accgttgatg
     6541 gtcgccgact tgatcgcccc gccctcgacc cc
//
LOCUS       DASTNK010000047        18901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_774215, whole genome shotgun sequence.
ACCESSION   DASTNK010000047 DASTNK010000000
VERSION     DASTNK010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 18901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18901
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774215"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFR20_03630"
     CDS             complement(<1..227)
                     /locus_tag="VFR20_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEU0229638.1"
                     /translation="MPSNTVAQFATELKMPANVLLEQLRSAGVELSSVDDAVTDADKA
                     KLLDSLRRSHGGDEGKKITLTRRQTSEIRQA"
     gene            complement(301..1791)
                     /gene="nusA"
                     /locus_tag="VFR20_03635"
     CDS             complement(301..1791)
                     /gene="nusA"
                     /locus_tag="VFR20_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEU0229639.1"
                     /translation="MSREILLLVDALAREKNVAREVVFGALENALASAMKKRFKEDAD
                     VRVSIDRVTGEHSGFRRWLVVPDDAGLQEPDQQELLSDAQEISPGIQVGEYIEESLEP
                     EEFGRIGAQAAKQAILQKIRDAEREQVLTDFLERGENIISGSVKRMDKGDVIVETGKI
                     EARLPRSEMIPKENIRVGDRIRAWVLRIDRAARGQQVILSRTSPEFIRQLFENEVPEI
                     EQGLLEIKAAARDPGVRAKIAVVAYDKRIDPIGTCVGMRGSRVTAVRNELGGEQVDIV
                     LWAEDPAEFVIGALAPAHVESILVDEDKHAMDVVVDAENLPKAIGARGQNVRLASELT
                     GWQINIMTPEESQNRQEEERSALRQTFIDRLDVDEEVADILIDEGFTGLEEIAYVPMQ
                     ELLEIEAFDEDTINELRTRARNALLTEAIAQEERVQKTTPDLLELEGMTPELAAKLAG
                     KEVFTRDDLAELSADELSEAAGLGEQESSDLIMRARAHWFENDDEA"
     gene            complement(1788..2276)
                     /gene="rimP"
                     /locus_tag="VFR20_03640"
     CDS             complement(1788..2276)
                     /gene="rimP"
                     /locus_tag="VFR20_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU0229640.1"
                     /translation="MTDIVALARQTLAGTGLELVDVERAPLGLLRVTIDRPEGVRIED
                     CEQVSRQLSRVFEVENIDYKRLEVGSPGVDRPLKTVADFQRFAGQRVEIKLRHAVDGR
                     RAFAGLLRAPGPAGDSADADPTFILELEAGSEGPGELGFAFHEVERARLDPVLDFKGK
                     KR"
     gene            complement(2739..4274)
                     /locus_tag="VFR20_03645"
     CDS             complement(2739..4274)
                     /locus_tag="VFR20_03645"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU0229641.1"
                     /translation="MVGASVEGATPESTEGKPGSPRGRGRKLRTPFRRRRGDAVASGA
                     QDASEAVPDAPPPVTSRKPRARKAVVRDNVGEKDAEQALAYLANEARLEQRLGKYLNS
                     DAVTPKLHKVLAEAGVGSRREMEELIVAGRVSVNGEPAHIGQRVAASDVVKVNGRVVA
                     RVNTHKPPRVILYHKPAGEIVSHDDPAGRASVFARLPKVRIGKWLSVGRLDLNTEGLL
                     ILTTSGDLANRLMHPRYGAEREYAVRILGELTDEQQASLTSGVDLDDGPAHFGSLTFL
                     GGEGSNRWYRVTLKEGRNREVRRMFETAGVTVSRLIRTRFGEVVLPRDLRRGRWDELE
                     PTMVTALMVQLGLIHDDDDGVGAKAGRSRQPASHDSALPPGFGTMERNGMNGARIGRR
                     GKLSGGRSGQVSSEHTYPSDPYGTGVMFSGGLANGHPDGGKAKKGGGRPKRGAAPKGP
                     QESRPARKTGRPKNGQETGSQPSKGGRGKSTPKRSADWQPRGASAHESHLGVIGRGRH
                     SRP"
     gene            complement(4344..5210)
                     /gene="scpB"
                     /locus_tag="VFR20_03650"
     CDS             complement(4344..5210)
                     /gene="scpB"
                     /locus_tag="VFR20_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEU0229642.1"
                     /translation="MTETEMIRVLETALLCADQPMPLSELRVLFPPPQEISNDDLRRL
                     LALLQLDWTDKGLELRNLATGWRFQSRPEMQRYLERLNPDKTPRYSRAVLETLAIIAW
                     RQPVTRGDIEDIRGVTVSSQIVKTLEDRGWIEVLGHRDAPGKPALLGTTRRFLDDLGL
                     KALDELPALESREEAAALAGLDLGDLPIEVVGMTGVEASDESPESGRANDEDDTLNAS
                     VGTGSGTSGESATVAWTDDGEADAPMSPPSDSVDVPDPEPDTSPVLSSVADDAGASTR
                     DGEWLADPGRAE"
     gene            5281..5357
                     /locus_tag="VFR20_03655"
     tRNA            5281..5357
                     /locus_tag="VFR20_03655"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5315..5317,aa:Met,seq:cat)
     gene            6414..7589
                     /locus_tag="VFR20_03660"
     CDS             6414..7589
                     /locus_tag="VFR20_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0229643.1"
                     /translation="MTDSFQLARHLADITAGHWNALAGDHPFLRHEYLLALEEAGCAT
                     SHTGWAPHYLLLRRDGHLAGAMPLYLKSHSRGEYVFDFAWAQAFAEHGLDYYPKLLGA
                     IPFTPVRGPRILAHNPEDRALLARQAIALARDNGLSSLHILFPSEDDRVALAAAGFMF
                     RENIQFHWFNHGHRTFDEFLASLSQPKRKKIRQDTKKVTEAGIRFRWLRGSQIDDRAL
                     RFFVRCYNHTYLEHGNPPYLNDAFFQRLRRDMPETLVLIVAELNGEPIATALNLRGGT
                     ALYGRYWGTVRFVPGLHFETCYTQAIRFCIEEGIPVFEGGAQGEHKLSRGLIPVETCS
                     AHWIRDQRYAAAIERYLQRETPAMREYAEMLRRHTPYRQTGPSGADPTPPESPESPD"
     gene            complement(7625..8179)
                     /gene="phaR"
                     /locus_tag="VFR20_03665"
     CDS             complement(7625..8179)
                     /gene="phaR"
                     /locus_tag="VFR20_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEU0229644.1"
                     /translation="MTQTQSSVSIRLIKKYPNRRLYDTQTSTYITLSDVKQLVLDNAA
                     FQVVDAKSGDDLTRSILLQIILEEESGGVPMFSATALSQIIRFYGHAMQGVMGTFLEK
                     NIQAFMDIQERVAEQSKGVYGGQFGPEAWAQFMNVQTPILQNMMNNYIDQSKNLFVQM
                     QDQMQDQTRSMFSTFPFKPAEPKK"
     gene            complement(8312..9049)
                     /gene="phbB"
                     /locus_tag="VFR20_03670"
     CDS             complement(8312..9049)
                     /gene="phbB"
                     /locus_tag="VFR20_03670"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEU0229645.1"
                     /translation="MSGKLAYVTGGMGGIGTSICQRLAKEGYNVVAGCGPSRNFKQWL
                     EEQAALGYKFHASVGNVSDWESTVQAFEHIKKDFGTVDVLVNNAGITRDGMFRKMTRE
                     DWRAVIDTNLNSLFNVTKQVIDDMVGNQWGRIINISSVNGQKGQFGQTNYSTAKAGIH
                     GFTMALAQEVASKGVTVNTISPGYIGTDMVRAIRPDVLEKIVGTIPVRRLGTPEEIGS
                     MVAWLASDDAGFATGADFSLNGGLHMG"
     gene            complement(9161..10795)
                     /gene="phaC"
                     /locus_tag="VFR20_03675"
     CDS             complement(9161..10795)
                     /gene="phaC"
                     /locus_tag="VFR20_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEU0229646.1"
                     /translation="MGAPSFSEWPVPTSIPPVKLAEIQAEFSREWSRITSEAQRGVLG
                     APLDARFSAQPWQANPAHLLTAHLYLLSARAMVQMVEAARVPEAVRRRLRFSAMQWVD
                     AASPANFLATNPEALEALIASHGQTLQRGIGKLFDDLKRGRVSQTDETHFRVGENVAT
                     TPGAVVFENKLFQLIQYAPLTEKTHRKPLVMVPPCINKFYILDLQQRNSFVRYAVEQG
                     FTVMLVSWRNPLPTDTDGADTATWADYLQDGVLRALDVAASITGQEQVNALGFCVGGT
                     LLASALALARARGRNPVGALTLLTTLLDFHDTGVLDVFVDESHAQMRECQLGQGGLMS
                     ARELATTFSFLRPNELVWNYFVNNYLKGEEPPAFDLLFWNADGTNLPGPFFCWYFRNT
                     YLENKLKIPGGVRVADQALDLSALDMPAYVFGSREDHIVPWRSAYASTDILRGPLRFV
                     LGASGHIAGVINPPAKKRRHYWTHARTYTHADIPADPDAWLAHADQHEGSWWPDWSQW
                     LASHGGELVRARSRLGNARHKPIEQAPGRYVAVRAV"
     gene            complement(10913..11725)
                     /gene="pgeF"
                     /locus_tag="VFR20_03680"
     CDS             complement(10913..11725)
                     /gene="pgeF"
                     /locus_tag="VFR20_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEU0229647.1"
                     /translation="MEHMNPADDTGSGGADLLAGLDLVSGLSWPGVRYFSTTRAGGMS
                     HGAFASLNLGAHTRDDPASVRENRRALRRVLPDEPLWLEQVHGSDVLDADAWLAERGS
                     APTADAAVTAQPGRVLAIMTADCLPVVLSDLDGTVLGVAHAGWRGLAGSVLEHTVARM
                     RSARPRAGSLRAWVGPAISQRHFEVGHDVYTAFVDQDPGVRMYFVRSPVSSKWLADLP
                     SIARHRLWRAGVEDVELSHYCTYGQADLFYSYRRASDTGRFATLAWLRPDGA"
     gene            complement(11713..12675)
                     /locus_tag="VFR20_03685"
     CDS             complement(11713..12675)
                     /locus_tag="VFR20_03685"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU0229648.1"
                     /translation="MPDTDTSADSVPEAVPLQFQVSLRALPERLDKVLAGLLPDHSRS
                     RLQTWIGAGHVLVNGLPGKIRQMINPGDRILVWEQVAPESLAFSPEPVDFEVVDQSSD
                     WIVVNKPAGLVTHPGAGNWHGTLLNGLLYRFPELLSVARAGIVHRLDKDTSGLLVVAR
                     NEKTQTHLVRQLQQRTVHREYVALVHGVVAAAGRVERDIGRDPRVPVRMTVDRPIAPK
                     PAVTHFAPRRSGEVEDGEVLTEIECRLETGRTHQIRVHLASLGHPLVADTLYGGRQVA
                     GATRQLLHARALSFDDFSTGRRVDIRIPLASDFQQILERVAWNT"
     gene            12786..13571
                     /locus_tag="VFR20_03690"
     CDS             12786..13571
                     /locus_tag="VFR20_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEU0229649.1"
                     /translation="MLVLAAAAVIAGCSSFKGEHDPTAGWSAPKLYKDARAQMSDANW
                     KDARTRLEAIESRYPFGIYAQQSLIDLAYVDWKDQEPEQALAAIDRFQQQYPNHPGTD
                     YMLYLKGLVTFTPPSAAFTRITQQDPSERDPKGLRESYAAFNELIKRYPNSPYTKDAK
                     QRVVWLVSTIAQHEVDVAQYYYQRGAYVAAANRAQKVITDFQGVPIAEKALYIMYLSY
                     GKLGLTKLQSDAKRVLDKNFPNSKYYKQGLNPPVDYWNPINWF"
     gene            complement(13654..15681)
                     /locus_tag="VFR20_03695"
     CDS             complement(13654..15681)
                     /locus_tag="VFR20_03695"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEU0229650.1"
                     /translation="MLLQELSDIFAADGPIASAIPNYRPRQAQLELAQAIEETMRERA
                     TLVAEAGTGTGKTWAYLVPAFLGGGKVLISTGTRTLQDQLFRRDVPRLRDALALPVAA
                     ALLKGRGNYVCHYHLGRLAGDERALKSRAEVTQLRRIQEFAVHTKTGDKAELASVPEE
                     ADIWSRVTSTRENCLGQDCPHVRDCFVLKARREAQEADLIVVNHALFMADLALREEGI
                     TDLLPEVETVVFDEAHQLPDVATRFLGTSVSSHQLLDLARTMQAAGLAHAREATNWGE
                     AGRSLEQATSELRLAAAPVERLPGRRATFQAIPESHQFDQALFALLERLDHCAALLGA
                     VAQAHPDLDTALKTCLDIRARLFRWSQPDRDGHDALAASMQAADSEEAAPPMGPAVRW
                     VEAGTHFLRLHSAPLSVANVFSRYRRRNQAWILTSATLSVQGRFDYFVGQLGLEDAET
                     RRWESPFDYATQGVLFVPKGLPVPGSPLFNERFVQVLVPVIQACGGGVLVLCTTLRAV
                     DHIAELLRDAFDEAGIERVVLRQGDGSRRILLDRFRSESNVVLVGSASFWEGVDVPGN
                     ALTLVAIDKLPFAPPDDPVLEARLNLCRSNGGNPFVEFQLPEAALSLKQGAGRLIRSE
                     TDWGVLMVGDGRLVDKPYGRQLWRGLPPFARTRDADQVVAFLRERSAAHPA"
     gene            15812..18184
                     /locus_tag="VFR20_03700"
     CDS             15812..18184
                     /locus_tag="VFR20_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HEU0229651.1"
                     /translation="MTETSTPTRTDAAAPSFDTDPARIVALLANELNVRPQQVAATVE
                     LLDGGATVPFIARYRKEATGGLDDTVLRNLEVRLIYVRELEERRQAILESVHQQGKLG
                     ADLERDIKAADTKQRLEDLYAPYKPKRRTRAQIAREAGLEPLADAILAERGCDPSKLA
                     VAYLNPEASIPDAKTALDGARDILAERFAENADLLADLREYLWNAGQLYSKVVDGKEN
                     DGNNFRDWFDFSEALRSLPSHRVLALLRGRQQGVLDLRIGLEPALDALTPHPCVERIA
                     RVLDIDAGFAADDPPRAVWLGDACRWTWRVKLLSAFETEFVTRLRESAEHEAIRVFAA
                     NLKDLLLAAPAGPKSVMGLDPGIRTGVKVAIIDATGKLLDTATVYPFEPRRDREGAMN
                     TLAALARRHDVELVAIGNGTASRETEKLVADMHARFPEPALTRVVVSEAGASVYSASE
                     LAAHEFPDLDVTLRGAVSIARRLQDPLAELVKIEPKAIGVGQYQHDVNQRELARALDA
                     VVEDCVNAVGVDVNTASAPLLARVSGLNTLLARNIVAWRDEHGAFANRKTLLDVSRFG
                     AKAFEQAAGFLRILNGDNPLDASSVHPEAYPVAERILKKINLDASRAMKQPGSLKGLS
                     PSEFTDDRFGLPTVKDIFTELEKPGRDPRPEFKTATFMEGVDTLQDLRVGMLLEGVVT
                     NVANFGAFVDIGVHQDGLVHISALAEQYVKDPRDVVRVGQAVKVKVQEVDVARKRIAL
                     TMRLNDDAPPARRATERGTGGTTAARRRSDRPAEPTNTGAMAAAFAKLRR"
     gene            complement(18265..>18901)
                     /locus_tag="VFR20_03705"
     CDS             complement(18265..>18901)
                     /locus_tag="VFR20_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229652.1"
                     /translation="SGSVPEAVAGLAVVILAILGLAGVSPTFLVAIATIVFGVELLLN
                     GASTMSDLGRVLGQEGETEMLDGSAVNGLSMVFLAGVAGIVLGILALLEVSSAHLVAI
                     AAIAFGGALLVSSNADMRIRMLETASHMHSRAGTELAEGVASDGAGMQTMSGLTAVVL
                     GILALAGFASVTLVLVALLTMSGYAVMSSGFISGSLLRAFGMGTQHAHHHM"
BASE COUNT         3785 a         6260 c         5480 g         3376 t
ORIGIN      
        1 tcggcctgtc ggatctcgga ggtctgccgg cgcgtcaagg taattttctt gccctcgtct
       61 ccaccgtggg aacggcgcag cgaatcgagc agtttcgcct tgtccgcgtc agtgacggcg
      121 tcgtcgaccg aactgagctc aacaccggcc gaacgcagtt gctccagcaa cacgttggcg
      181 ggcattttca gttcggtggc gaactgggcg acggtattac tcggcattcg actctctctt
      241 gcttgctaca actatgtcaa tgatgaatac agcccgatcc gcgcatccac aacggtgcga
      301 tcaggcctcg tcatcgttct cgaaccagtg tgcccgggcg cgcatgatca gatcgctgga
      361 ttcctgctcg ccgaggcctg cggcttcgga cagctcatcg gcggacagtt cggccagatc
      421 gtcccgggtg aagacctctt tcccggccag cttggcagcg agttccgggg tcatgccttc
      481 gagttccagt agatcgggcg tcgtcttctg cacccgctct tcctgggcga tcgcctcggt
      541 caacaatgcg ttgcgggccc gcgtgcgtag ttcgttgatc gtgtcttcgt cgaaggcctc
      601 gatctcgagc agttcctgca taggcacgta cgcgatttcc tccaggccgg tgaaaccttc
      661 atcgatgagg atatcggcga cctcttcatc cacatcgagg cgatcgatga aggtctgacg
      721 caatgccgac cgttcttctt cctggcgatt ctggctttcc tcgggcgtca tgatgttgat
      781 ctgccaaccg gtgagctcgg acgccaggcg tacattctga ccccgggcac caatggcctt
      841 gggcagattc tcggcgtcga ccacgacgtc catggcgtgt ttgtcctcat cgacgagaat
      901 ggactcgacg tgcgcgggcg ccaaggcgcc aatgacgaat tcagccggat cctccgccca
      961 cagcacgatg tcgacctgtt cgccgccaag ttcattgcgc accgccgtca cacgcgaacc
     1021 gcgcattccc acgcaggtgc cgatgggatc gatacgtttg tcataggcga caaccgcgat
     1081 cttggcgcgc acgccgggat cccgcgccgc ggccttgatt tcaagcaggc cttgttcgat
     1141 ttccggcact tcgttttcaa agagctggcg aatgaactcg ggcgaggtcc gcgacaggat
     1201 gacctgctgg ccgcgcgcgg cgcggtcgat acgcagcacc caggcgcgga tgcggtcgcc
     1261 cacccggatg ttttccttgg ggatcatttc agagcgcggc aggcgcgctt cgatcttgcc
     1321 ggtttcgaca atgacgtcgc ccttgtccat gcgcttgacc gaaccggaaa tgatgttctc
     1381 gcctcgttcc aggaaatcgg tcagcacttg ttcgcgctcg gcgtcgcgga tcttctgcag
     1441 gatggcctgt ttcgccgcct gcgcgccgat acggccgaat tcctcgggct cgagcgattc
     1501 ttcaatgtac tcgcccacct ggattcccgg cgagatctcc tgggcgtcgg acagcaattc
     1561 ctgctggtct ggttcctgca ggcccgcgtc atcgggcacc accagccaac ggcggaatcc
     1621 cgaatgctcg ccagtgaccc gatcaatgga cacgcgaacg tccgcgtctt ccttgaagcg
     1681 ctttttcatc gccgacgcca aggcattctc cagggcgccg aacacgactt cgcgtgccac
     1741 atttttttca cgcgccaagg cgtcaaccaa tagaagaatt tcgcgactca tcgcttttta
     1801 cctttgaaat caagaacggg gtcgagtcgg gcacgctcga cttcgtgaaa cgcaaagcct
     1861 agttcgccgg gcccttcgga tccggcctcg agctcgagga tgaacgtggg atcggcgtcc
     1921 gcggagtctc cggcgggccc gggcgcgcgc aacagtccgg cgaaggcgcg acgcccatcg
     1981 accgcgtgcc gcaacttgat ctcgacgcgt tgaccagcga aacgctggaa atcggccacg
     2041 gtcttcagcg ggcggtcaac gccgggcgag ccaacctcga gccgcttgta atcgatgttc
     2101 tcgacctcaa acacacgcga aagctgacgc gacacctgtt cgcagtcttc gatccgaacc
     2161 ccttcagggc gatcgatcgt cacacgcagc aacccgagcg gcgcgcgctc gacgtcgacc
     2221 aattcaaggc cggtgccagc aagcgtctgt cgagccaaag cgactatatc cgtcatacca
     2281 gaaattcaaa aaaaaaatgg gctgcacgcc cagcccatta ttatcacgcc tgccgtaccg
     2341 caaaaccgga caccgtggaa cccttgcacc cgcatggcgc acgcccatac ggccagcagg
     2401 cctcgcacca gcacgatcgc aagccccata acgccacccg cggccagaca acatcgcaaa
     2461 tagccggcta acattgaccg ctgattgata ttgcccgctg tcgctgattg atattgccaa
     2521 ctgtatttgc tgggctttat ttgccggcct tatttgccaa taaagcccgg aataaaacca
     2581 gtaaagccgt tcattttagc agaaaattga aaaaattcct gctaagtcac agccttacac
     2641 aaaacatcgg cattcacggc ccggcgaggc gacgccgccc tcccccgaac ccaccggttc
     2701 atgtgcgaca cgcccgcgtc agatcacacc caggggcctc agggccgcga atgacgccct
     2761 cgcccaatga cccccaaatg ggattcatgc gccgacgcgc cgcgcggctg ccaatcggcg
     2821 gagcgcttcg gcgtcgattt gccccgcccc ccttttgatg gctgggatcc ggtttcctgc
     2881 ccgttcttcg gccgccccgt ctttcgagcc ggacgcgatt cctgcgggcc ttttggcgcc
     2941 gcgccccgct tcgggcgccc gccgcccttt ttcgccttgc caccgtccgg atgaccgttg
     3001 gccaatcctc cgctgaacat caccccggtc ccgtaagggt cggacgggta agtgtgttca
     3061 gagctgactt gaccactccg gcctccggag agcttcccgc ggcggccgat acgagcgccg
     3121 ttcatgccat tgcgctccat cgtgccgaac cccgggggca gggcactgtc gtgtgacgcg
     3181 ggctggcgcg accgccccgc tttggcaccg acgccgtcat catcgtcgtg aatcagaccc
     3241 agctggacca tcaacgccgt caccatcgtc ggctcgagct cgtcccagcg accgcgacgc
     3301 agatcacgcg gcaacaccac ttcgccaaag cgcgtacgga tgagacggct gaccgtcacc
     3361 cccgctgtct cgaacatgcg gcggacctcg cgattacgcc cctctttgag cgtcacacgg
     3421 taccagcgat tgctcccctc gccaccgagg aacgtcagtg agccaaaatg cgccgggcca
     3481 tcatccaggt cgacacccga ggtcaggctg gcctgctgct cgtcagtcag ctcccccaat
     3541 atccgcacag cgtattcccg ctccgcgcca taacgcggat gcatcagccg gttggccaga
     3601 tcccccgaag tggtgagtat cagcaacccc tcggtgttca gatccagacg ccccaccgac
     3661 agccattttc cgatccggac cttcggcagg cgcgcaaaca cgctggcccg cccggccgga
     3721 tcatcatggc tgactatctc accggccggc ttgtgataga gaatgacgcg aggcggctta
     3781 tgcgtattga cgcgtgccac cacacgccca ttcaccttca ccacgtcgct ggcggccaca
     3841 cgctgaccaa tatgggctgg ctcgccattg acggacaccc ggccggccac gatgagttct
     3901 tccatttcgc ggcgcgagcc cacgccggct tcggccaaaa ccttgtgtag cttcggcgtc
     3961 accgcgtcgc tgttcagata tttacccagg cgctgctcaa gccgtgcttc gttggccagg
     4021 tacgccaacg cctgttccgc atctttttca ccaacattgt cgcgcacgac cgccttgcga
     4081 gcccggggtt tgcgagacgt cacgggcgga ggagcatccg gtaccgcctc ggacgcatcc
     4141 tgcgccccgc ttgccacggc atcgccccgc cgccgacgaa atggcgtcct gagtttgcgt
     4201 ccgcgcccgc gcggcgagcc tggcttcccc tcggtggact cgggtgtggc gccttcgacc
     4261 gatgcgccga ccattgtgtc attgtgctgt tctgattcac tcattttctt caaaatactc
     4321 cgtatcgtta ttctttattc caattattcc gctctacccg ggtccgccag ccactcacca
     4381 tcccgcgtcg acgcccccgc atcatcggcg acgctgctca gtactggtga cgtgtccggc
     4441 tccggatcgg gcacatcgac actatccgac ggcggtgaca taggggcgtc cgcctcccca
     4501 tcatcggtcc atgcgacggt tgcgctctct ccagacgttc ctgaacccgt cccgaccgac
     4561 gcattcagcg tatcgtcctc gtcgttcgcc ctgcctgact ccggggattc atcggaagcc
     4621 tcgacccccg tcattcccac gacctcgata ggcaaatccc ccagatccaa cccagccaac
     4681 gcagccgcct cttcccgtga ttccagcgcc ggcagttcgt cgagcgcctt gaggccgaga
     4741 tcgtccagaa accgccgcgt cgtacccagc aatgccggtt tcccgggggc atcgcggtgt
     4801 ccaaggacct caatccaccc acggtcctcc aaagtcttga cgatctgcga cgacacggtc
     4861 acgccccgta tgtcttcgat gtcgccgcgt gtgacgggct gacgccaggc aatgatggcc
     4921 aacgtttcca gaacggctcg cgaatagcgg ggggtcttgt ccggattgag gcgctccagg
     4981 taccgctgca tctccggacg actctggaac cgccaaccgg tggccagatt gcgcaactcc
     5041 agccctttat ccgtccagtc gagctgcaac aacgcaagca gccgcctgag atcgtcattg
     5101 gaaatttcct gcggcggagg gaacaagacc cgcaattcgg acaagggcat aggctggtcg
     5161 gcgcacaaca gcgcggtttc cagcactcgg atcatctcgg tttctgtcat tcgtgaaggg
     5221 ggccatgccc gccagtttgc ataaacttca aatcaagtct aaaatagctt tctttccaga
     5281 cgcggggtgg agcagtctgg cagctcgtcg ggctcataac ccgaaggtcg taggttcaaa
     5341 tcctgccccc gcaaccagtt cccagaaccg gccgcttgca accatgcagc ggccggtttc
     5401 gttttaacaa gcggttttaa ccagcggtgg ttttggccag cggtttcgcc acgagtgcgc
     5461 gcccgtggcg caaaaaccat gacgccatgc ccgcgccggc cacgcacgtg cgcaaacgcg
     5521 ccattcatgg ggcgggcgcg agtcatgctt gcatttccag aaaaatacga tccatgcttt
     5581 ccattgttcc gacattcggt agacattcgg cacacccgcc tctgccagga agggtgccac
     5641 ggtcgaacga gcggactgaa aaaacgccaa tcaaggaagc taatcaagga cgccgataaa
     5701 aacgccaata aaggacgcca atcgtgatgc ccagcctgac gccagccggg acgccagttg
     5761 gcgccatctg ggtgccgaca aagggacacc agtaagagga acgccaacaa gaggaccaac
     5821 aagaggacca acaagaggcg ccaacaaagg gagcaccaac aaagccagca gagacaccgg
     5881 cggagaggcc cgcacatcac tgacgaggcg ttcccggaac atcaaagagc attattcggg
     5941 cagcttgacg accgcatcca ggtcgtcgag caaactcgag gaacaaattc aagaaaccaa
     6001 ccttggggaa ccgcacaggg gaaccgcatg gccggggaat tgcatgggca aacgccatga
     6061 cacacatccg gccacgtccc cactccaagc tcgggggtgc gctgaaaatt tattcggcgt
     6121 taaattattt gcctgcccat cgcggcggat accggcatca ccacgccacc gagggaacac
     6181 cccattcgga ttgggcttcc gggttggcgg gccaccaaaa catgccatgc cacgatcttg
     6241 ggctgccaga cggctgcgat tattggcgcg gctcgcattg caatcactga tcaggttacc
     6301 attacaccat ccacgggcaa tgaatgcaac agcggcaacc ctgcgcgctg tcaccgcgca
     6361 acaccgcggc actgccagtc ttgcacgtca agccaacgcc cggactcacg aaattgactg
     6421 attcctttca gctcgctcgc caccttgccg acatcaccgc tggccactgg aatgcgctgg
     6481 caggcgatca cccattcctg cgccacgaat atctgctggc gctggaagag gcaggctgtg
     6541 ccacatcgca cacggggtgg gcgccgcatt atctgctgct caggcgcgac ggccacttgg
     6601 cgggcgccat gccgctttat ctcaagtcac attcccgtgg cgaatatgtg tttgatttcg
     6661 cctgggcaca ggctttcgcc gaacacggac tagactatta ccccaagctg ctcggcgcca
     6721 tacccttcac ccctgtccgg gggccgcgca ttctcgctca caatccggag gaccgtgccc
     6781 tactggcgcg ccaggccatc gccctggcgc gcgacaacgg actgtcgtca ctgcatattc
     6841 tgtttccgtc agaagacgac cgtgtcgctt tggccgccgc tggcttcatg ttcagggaaa
     6901 acatccagtt tcactggttt aatcatgggc acagaacatt tgacgaattc ctggccagcc
     6961 tgagccaacc caagagaaaa aaaatccggc aggatacgaa aaaagtgact gaagccggca
     7021 tacgattccg ctggctgcgt gggagccaga tcgacgaccg cgctctgcgc ttttttgtcc
     7081 gatgctacaa ccacacctat cttgagcacg gcaatccccc gtatctcaat gacgcgttct
     7141 tccagcgtct gcgccgcgac atgcccgaga ccctggtcct gatcgtcgcg gaactgaacg
     7201 gcgaaccaat tgccaccgca ctgaacctgc gtggcggtac ggccctgtat ggacgctact
     7261 ggggaaccgt ccggttcgta cccgggctcc acttcgaaac ctgctacacg caggcgatca
     7321 ggttttgcat cgaagagggg attccggttt tcgaaggtgg cgcgcaagga gagcacaagt
     7381 tatcccgcgg cctgattccc gtggaaacct gttcggccca ttggatacgg gatcaacgct
     7441 acgccgccgc gatagagcgc tatctgcaac gcgagacgcc agccatgcgg gaatacgccg
     7501 agatgttgcg tcgacacacc ccatatcgac agaccgggcc aagcggcgcc gaccccacgc
     7561 cacccgaatc acccgaatca cccgattgaa ctttgaccac ccgccggcgc gtgggcgcac
     7621 cagcctattt tttgggttca gccggcttga acggaaaggt ggagaacatc gagcgggtct
     7681 gatcctgcat ctggtcctgc atttgcacga atagattctt gctctggtcg atatagttgt
     7741 tcatcatgtt ctgcaggata ggcgtttgca cgttcatgaa ttgcgcccat gcctcgggcc
     7801 cgaactgccc gccgtacacc cccttcgact gctcggcaac acgttcctgg atgtccatga
     7861 atgcctggat gttcttctcc aggaacgttc ccatcacacc ctgcatggcg tggccgtaga
     7921 accggatgat ctgggacagc gccgtggctg agaacatagg cactccgccg ctttcctctt
     7981 caaggatgat ctgcagcagg atgctacggg tgaggtcatc gccggatttg gcgtcaacca
     8041 cctggaaagc cgcattgtcg agcaccagtt gcttgacatc tgacaacgtg atgtaggtgc
     8101 tggtctgcgt gtcgtacagg cgacgattag ggtacttttt gatcaggcgg atggaaacgg
     8161 acgattgggt ttgtgtcatg tcagtaattc ggtatgccta tccaggatcg aaattgaaaa
     8221 gcatcgggcg cccattattc atgggacgcc gcgacgcttc accgaaggtg ccatcgtagg
     8281 gacgatgata ccggaaccac gccgtgggga atcagcccat gtgcagacca ccgttcagcg
     8341 aaaaatcggc gcctgtggca aacccggcgt catcggatgc cagccaggcg accatcgaac
     8401 cgatttcctc gggcgtgccc agacggcgca ccggaatcgt gcccacgatt ttttcaagca
     8461 cgtcgggacg aatggcccgg accatgtctg taccgatata tcccggcgat atcgtgttga
     8521 cggtgacacc cttggacgcc acttcctggg ccagcgccat ggtgaaacca tgtatgcccg
     8581 ccttggcggt ggaatagttg gtctggccga actggccttt ctggccattg accgaactga
     8641 tgttgatgat acgcccccac tggttgccga ccatatcgtc gatcacttgc ttggtgacgt
     8701 tgaacaaact gttgaggttg gtgtcgatca ccgcgcgcca gtcttcacgg gtcattttac
     8761 ggaacatgcc gtcgcgcgtg atgcccgcgt tattgaccag gacgtccacc gtaccgaaat
     8821 ccttcttgat atgctcgaac gcctgcacgg tggattccca gtcggagaca ttcccgaccg
     8881 aagcatggaa cttgtagccc aacgccgcct gctcttcaag ccattgcttg aaattgcggc
     8941 tcggcccaca tcccgccacc acgttataac cttctttggc caggcgctgg cagatcgagg
     9001 tgccgatccc acccatgccc cccgtcacat aagccagttt tccactcatc accgcctcct
     9061 agacaaaatt acacccggtc gcataccacg atgggacgga ccggcatgac acgcgaaacc
     9121 gcttcccatt tcagcgccgg gccggaccgc tgtgccggcg tcatacagct ctgacggcga
     9181 catagcggcc gggagcctgt tcaatgggtt tgtggcgcgc gttgcccagc ctggaccgcg
     9241 cgcgcaccaa ttcaccgcca tgggaagcca accattggct ccagtcgggc caccagctgc
     9301 cctcgtgctg gtcggcatgc gccagccagg cgtcgggatc ggccggaatg tcggcgtggg
     9361 tgtaggttcg tgcgtgcgtc cagtagtggc gtcttttctt ggcgggggga ttgatgacac
     9421 cggcgatgtg gccggaggcg cccaggacga aacgcagggg accgcgcaga atgtcggtgc
     9481 tggcgtaggc gctgcgccag ggaacgatat gatcttcgcg cgaaccgaaa acataggccg
     9541 gcatgtccag ggcgctgaga tcgagcgcct gatcggccac ccgaaccccg cctggaatct
     9601 tgagtttgtt ttcgagatag gtattgcgga aataccagca gaagaacggc cccggcagat
     9661 tggtgccgtc ggcattccag aaaagcaggt cgaaggctgg cggttcttcg cctttgagat
     9721 aattgttgac gaagtaattc cagacgagtt cgttgggccg aaggaatgaa aacgtcgtgg
     9781 cgagttcgcg cgcggacatc aaccccccct gcccgagctg acattcgcgc atctgggcat
     9841 gggattcgtc gacaaacacg tccagcaccc cggtatcgtg aaaatcaagc agtgtggtca
     9901 gcaacgtgag agcgccgacg ggattgcgcc ctcgcgccct ggcaagcgca agcgccgagg
     9961 ccagcagtgt gccgccgaca cagaacccca gcgcattgac ttgctcctgg cccgtgatcg
    10021 atgcggcgac atcgagggcg cgcaacacgc cgtcctgcag ataatcggcc catgtggcgg
    10081 tgtcagcccc atcggtatcg gtcggcaggg ggttgcgcca ggatacgagc atgaccgtga
    10141 agccttgctc gaccgcatag cgcacgaatg agttccgctg ctgcagatcg aggatgtaga
    10201 acttgttgat gcaaggcggc accatcacca gcggtttccg atgcgttttt tcggtgagtg
    10261 gcgcgtactg gataagctgg aacagcttgt tctcgaagac caccgccccc ggcgtggtgg
    10321 ccacgttttc gcccacacgg aaatgggttt catcggtttg cgaaacccgc cctctcttca
    10381 gatcatcgaa tagtttcccg atgcctcgct gaagcgtctg gccatgactg gcgatgagag
    10441 cctcgagcgc ttcgggattg gtggcaagga aattggcggg tgacgcggcg tcgacccatt
    10501 gcatggccga gaaccgcaac cgacggcgca cggcctctgg cacacgggcg gcctcgacca
    10561 tttgaaccat ggcgcgcgcg gacagcagat agagatgcgc ggtgagcaag tgggcggggt
    10621 tggcctgcca cggctgcgcc gagaaccgcg catcgagcgg cgcgcccagc acaccgcgct
    10681 gcgcttcgga cgtgatgcgc gaccattccc gtgagaactc ggcctggatt tccgccagct
    10741 tgaccggcgg aatgctggtc gggacaggcc attctgaaaa agacggggcg cccacgttgt
    10801 acacctttgt gttgaactct tttgagtcaa tggtgggggg caattgaaat ggtgtcaatc
    10861 agggtaagtc agggtaagcc ccggcatcac caccacaggc caacccacac agtcaggccc
    10921 catcgggccg cagccacgcc aatgtggcga agcgcccagt gtcgctggcc cgacgatagg
    10981 aatagaacag atcggcctgg ccgtatgtgc aatagtggct cagttcgacg tcctcgacac
    11041 cggcgcgcca cagccgatgg cgcgcgatcg aaggaagatc ggccaaccat ttcgacgaga
    11101 ccgggctgcg gacgaaatac atgcgaaccc ccggatcctg gtcgacgaag gccgtataaa
    11161 cgtcatgtcc cacctcaaaa tggcgctggc tgatggcggg gccgacccag gcgcgaagcg
    11221 accccgcgcg aggtcgcgcg gaccgcatgc gcgccacggt gtgctcgagc acggaccccg
    11281 cgagcccccg ccatcccgca tgcgccacac cgagcacggt gccgtccagg tcggacaaga
    11341 ctaccggcag gcaatcagcc gtcatgatgg cgagcacacg accgggctgg gccgtcacgg
    11401 cggcatccgc cgtaggtgcc gagccgcgct cggcaagcca cgcatccgca tccagcacgt
    11461 cgctgccgtg cacctgctcc aaccacaacg gctcatccgg caacacgcgg cgcaatgcgc
    11521 gccgattctc ccgtacgctg gcgggatcgt cgcgtgtgtg cgctcccagg ttcagcgacg
    11581 cgaacgcgcc atgactcatg ccacccgcgc gggtggtgga aaaatagcgc acacccggcc
    11641 aggagagccc tgacacgaga tcaaggccgg ccagaagatc cgccccgccg gagcctgtgt
    11701 catccgcggg attcatgtgt tccatgccac tctctccaga atctgttgaa aatccgacgc
    11761 cagcggaatc cggatatcca cgcggcgacc agtggaaaaa tcgtcgaaac tgagcgcgcg
    11821 ggcatgcaaa agctgtcggg tggcgcccgc cacctggcgc cctccataaa gcgtgtcggc
    11881 caccagcgga tggccgaggc tggccagatg cacgcgaatc tgatgtgtgc ggccggtttc
    11941 gagccgacac tcgatctcgg tgagcacctc gccgtcctcg acctccccgc tgcggcgcgg
    12001 cgcgaagtgc gtgaccgcgg gcttgggcgc gatggggcgg tccaccgtca tgcgcaccgg
    12061 cacccgggga tcgcggccga tgtcgcgctc cactctaccc gcggcggcca ccacgccatg
    12121 caccagggcg acatactcgc gatgcacggt acgctgctgc aactggcgca ccagatgggt
    12181 ttgtgttttc tcgttgcgcg ccaccaccag cagccccgac gtatccttgt ccagccgatg
    12241 cacaatcccc gcgcgcgcca ccgacagaag ttccgggaac cgatacagca agccgttgag
    12301 caatgtgccg tgccagtttc ccgcccccgg atgcgtgacc agcccggcgg gcttgttcac
    12361 gacgatccag tcgctgcttt gatcgacgac ttcgaaatcg accggctcgg gcgagaacgc
    12421 cagcgattcg ggcgccactt gctcccagac caggatgcgg tcaccgggat tgatcatctg
    12481 ccggatcttg cccggcaggc cattgaccag cacatgccca gcgcctatcc aggtttgcaa
    12541 ccgactgcgc gagtgatccg gcagaagccc cgccaaaacc ttgtccagcc gctcgggcag
    12601 ggcccgcaac gacacctgga actggagcgg gacagcctcg gggacgctgt cagcggaagt
    12661 atctgtgtca ggcatggcga caaatcatat aatctgccct gcatgctaac accaaggacg
    12721 ctttcgtgac ccgcaccgtt accgacacat cccgcttcgc cggcttcagg cggttcgcgc
    12781 aatttatgct cgttctagcc gctgcagcag tcattgccgg ctgcagctcc ttcaagggcg
    12841 aacacgaccc caccgccggt tggagcgccc ccaagctgta caaagacgca cgggcgcaaa
    12901 tgagcgacgc caactggaag gacgcccgca cccgtctgga agccatcgaa tcgcgctacc
    12961 cattcggcat atacgctcag caatcactca tcgatctggc ctacgtggac tggaaggatc
    13021 aagaacccga acaggccctt gcggcaatcg accggtttca acagcaatac ccgaaccacc
    13081 cgggcactga ttacatgctc tacctgaagg ggctggtcac cttcacgcca cccagcgccg
    13141 ccttcacccg catcacccag caggatccca gtgaacggga ccccaaaggc ttgcgcgagt
    13201 cgtatgcggc attcaacgaa ctgatcaagc gctaccccaa cagcccctac acgaaagacg
    13261 cgaaacagcg ggtcgtgtgg ctggtcagca cgatcgccca gcacgaggtc gacgtcgccc
    13321 aatactatta tcagcgtggc gcgtacgtgg ccgcagccaa tcgcgcacag aaagtcatca
    13381 ccgacttcca gggcgtgccc atcgccgaga aagccctcta catcatgtac ttgtcgtacg
    13441 gcaagctggg gctgaccaag ctgcaaagcg atgccaaacg ggtgttggac aagaacttcc
    13501 cgaacagcaa atactataaa caggggctca acccgccggt cgactactgg aaccccatca
    13561 actggttctg atgtgctgtc tggtcggttc ctccacctga ccttgcgcct gggctcgccg
    13621 cctgcgggcg gtggcaatgc cgctgaatga gtctcaagca gggtgcgcgg cactccgctc
    13681 ccgcaggaac gccacgacct gatcagcgtc acgcgtgcgg gcgaatggcg gcagccctcg
    13741 ccatagctgt cggccatacg gcttgtcgac cagcctgccg tcccccacca tcaacacacc
    13801 ccaatcggtt tccgaacgaa tcagtcggcc cgccccctgc ttgagcgaca aggcggcttc
    13861 gggcaactgg aattccacga acgggtttcc gccgttcgac cgacacagat tcagccgggc
    13921 ctcgagcacg ggatcatcag gcggggcgaa gggcagcttg tcgatcgcaa ccagtgtcag
    13981 ggcattaccg ggcacgtcca ccccttccca gaaactggcg ctgccgacca gcaccacatt
    14041 gctttcggag cgaaaccggt caagcaggat acggcgcgaa ccatcgcctt gacgcaggac
    14101 gacgcgctcg atgccagcct cgtcgaatgc gtcacgaagc aattcggcga tatgatccac
    14161 ggcccgaagc gtcgtgcaca acaccagcac accgcctcca caggcttgaa tcaccggcac
    14221 caacacctgc acgaaccgct cgttgaacag cgggctcccg ggcacgggca gccctttggg
    14281 gacgaacagc acaccctggg tggcgtaatc gaacggtgac tcccaacggc gcgtctccgc
    14341 gtcctcgagt ccgagctggc cgacaaaata atcgaaacgg ccttgcaccg acagcgtggc
    14401 cgacgtcagg atccaggcct ggttgcggcg tcgataccgg gaaaaaacat ttgccactga
    14461 cagcggcgcg ctgtgcaggc gcagaaaatg cgtgcccgct tccacccagc gcacagccgg
    14521 gcccatcggg ggtgcggcct cttcggagtc cgccgcctgc atcgaggcgg ccaaggcatc
    14581 gtggccgtcg cgatctggtt gcgaccaacg aaacaaccgg gcacgaatgt ccaggcacgt
    14641 tttgagcgcg gtgtccaggt cgggatgtgc ctgcgccact gcgccaagca gcgccgcgca
    14701 gtgatcgagc ctttcaagca atgcgaacag cgcctggtcg aactgatgcg attcgggtat
    14761 ggcctggaaa gtggcgcgcc ggccagggag cctttccacg ggggccgccg ccagtcgcag
    14821 ctcgctggtc gcctgctcca gactccgccc cgcctccccc caattcgtcg cttcccgggc
    14881 atgcgccagg cccgccgcct gcatcgtgcg cgccagatcc agcagctggt gcgaagacac
    14941 actggtaccc agaaaccggg tcgccacgtc gggaagctgg tgcgcctcat cgaagacaac
    15001 cgtctcgacc tcgggtagaa gatcggtgat cccttcttcg cgcaatgcca gatccgccat
    15061 gaacagcgca tgattgacca cgatcagatc cgcctcctgc gcctcccggc gagccttcag
    15121 cacaaagcag tcgcgcacgt gcgggcagtc ctggcccaga cagttctcgc gagtcgaggt
    15181 gacgcggctc cagatgtcgg cctcttcggg cacggaggcg agttcagcct tgtcgccggt
    15241 tttcgtatga acggcgaact cttggatacg gcgcaactga gtgacttcgg cgcgtgattt
    15301 gagggctcgc tcatcaccgg ccagacgccc cagatggtag tggcagacat aattgccccg
    15361 gcccttgagc aacgccgcgg ccaccggcag cgccagcgcg tcgcgcagcc gtggcacatc
    15421 gcgccgaaac aactgatcct gcaaagtgcg ggtaccggtg gaaatcaaca ccttgccgcc
    15481 acccaggaag gccggcacga gatatgccca ggtcttgccg gtgcccgtac cggcctcggc
    15541 caccagcgtg gcgcgctcgc gcatcgtctc ttctatcgcc tgggccagct ccagttgcgc
    15601 ctgccgcggg cgatagttgg ggatggcgga ggcgatgggc ccgtcggcgg cgaaaatatc
    15661 ggacagttct tgcagaagca tgaaggcgcg gccggatacg gcctgtcata aaacctcaag
    15721 gaatcacacg tcatgataac cgtgtcggcc gacgtcaact gtccgcttgt ggcaaaatgc
    15781 cccgcttgac cgtgcctcat tctccgattt catgaccgaa acatccaccc cgacgcggac
    15841 tgatgccgcc gcgccatcgt ttgacaccga tcccgcgcgt attgtcgcac tgctggccaa
    15901 cgagctgaac gtgcggccac aacaggtcgc cgcgacggtc gaactgctcg acggcggtgc
    15961 cacggtgccg ttcatcgcgc gctatcgcaa ggaagccact ggcggactgg acgacaccgt
    16021 gctgcgcaac ctggaggttc gcctcatcta cgtgcgcgaa ctcgaggaac gccgccaggc
    16081 gatcctcgaa tcagtccacc agcagggaaa gctcggggcc gatctggaac gggacatcaa
    16141 ggcggccgac accaagcaac gcctcgagga cctttacgcg ccttacaagc caaaacgccg
    16201 cacacgcgcc caaatcgcgc gcgaagccgg gctggagccg ctggccgacg ccattctcgc
    16261 cgaacgcggc tgcgatccgt ccaagctcgc tgtggcctac ctgaacccag aagcatcgat
    16321 tcccgacgcg aaaacggcgc tggacggcgc gcgcgacatt ctcgcggagc gcttcgccga
    16381 gaacgccgat ctgctcgccg acctccggga atacctatgg aacgcgggac aactctattc
    16441 caaggttgtc gacggcaagg aaaacgacgg caacaacttc cgcgactggt ttgatttcag
    16501 cgaagcactg cgctcccttc cttcgcaccg tgttctggcc ttgttgcgcg gacgccagca
    16561 aggcgttctc gacttgcgca tcggcctgga accagcgctc gatgccctca cgccccaccc
    16621 gtgcgttgaa cgcatcgcca gggtgctcga catagacgcc ggcttcgcgg cggatgatcc
    16681 accacgcgcc gtgtggctgg gcgacgcgtg ccgctggacg tggcgcgtca aattgctgtc
    16741 ggcattcgaa accgagttcg tgacacgcct gcgtgaaagc gccgagcacg aagccatccg
    16801 cgtgttcgcc gccaatctca aggacctcct gctggcggcg ccggcggggc ccaagtcggt
    16861 catgggcctg gatcccggca tacgcaccgg cgtcaaggtc gccatcatcg acgccaccgg
    16921 gaaactgctc gacaccgcaa ccgtctaccc attcgagccg cggcgcgacc gcgaaggagc
    16981 gatgaacacg ctcgcggctc tggcccgccg ccacgatgtc gagctggtgg ccatcggcaa
    17041 cggcaccgcc tcacgggaaa ccgagaagct cgtcgccgac atgcacgcgc ggttccccga
    17101 gcccgccctg acacgcgtcg tcgtatcgga agcgggggcg tcggtgtact ccgcttcgga
    17161 actcgccgcg cacgagttcc ccgacctgga cgtcaccctg cgcggtgccg tctccatcgc
    17221 gcgacgcctt caggaccctc tcgcggaatt ggtgaagatc gaacccaagg ccattggagt
    17281 ggggcagtat caacatgacg tcaaccagcg cgaactcgcg cgcgcgctcg atgcggtggt
    17341 ggaggactgc gtcaacgccg tgggtgtgga cgtcaatacg gcatcggcac cgttgctggc
    17401 cagagtctcg ggactcaaca ctttgttggc gcggaacatc gtggcctggc gcgacgagca
    17461 cggggcattc gccaatcgaa agacattgct cgacgtctca cgcttcggcg cgaaggcctt
    17521 cgagcaagcg gcggggttcc tgcgcatcct caacggcgac aacccgctgg atgcgtcgtc
    17581 ggtgcacccc gaggcctatc cggtcgcaga gcgcatcctc aagaagatca atctggacgc
    17641 aagccgcgcc atgaagcagc ctggttcgct caagggcttg tcaccctctg aattcaccga
    17701 cgatcgcttt ggcctgccca ccgtcaagga catcttcacc gaactggaaa agccggggcg
    17761 tgatccccgg ccggaattca agaccgccac cttcatggaa ggtgtcgaca cactccagga
    17821 tctgcgcgtc ggcatgctcc tggaaggggt ggtcaccaac gtggccaatt tcggtgcttt
    17881 cgtcgacatc ggcgttcatc aggacggcct ggtgcatatt tcggcactgg ccgagcagta
    17941 cgtgaaggat ccacgcgacg tcgtgcgtgt cggccaggcg gtgaaagtca aggttcagga
    18001 agtggacgtg gcgcgcaaac gcatcgcgct caccatgcgg cttaacgatg acgccccccc
    18061 cgcgcgccgc gccacagagc gtggcacggg cggcacaacg gcggcgcggc ggcgatcgga
    18121 ccgcccggcc gagccgacga acacgggagc catggcggcc gcgttcgcca agctgcgtcg
    18181 atgacaaagc gggggccgga tcgaattccc gatccggccc ccgacaaccg ctcaatattg
    18241 aggcaacaag acgaccgcag tgtgtcacat atgatgatgc gcgtgctgcg tacccatccc
    18301 gaacgcccgc aatagactgc cgctgatgaa gccactggac atcacggcgt agccgctcat
    18361 ggtcaacaag gcgaccagca cgagcgtgac cgaagcaaag ccagccaggg cgagaatccc
    18421 caggacaacg gcggtcaggc cggacatcgt ctgcatgcca gcgccatcgg acgcaacgcc
    18481 ctcggccagt tccgtaccgg cgcgactgtg catgtgagaa gctgtctcga gcatccggat
    18541 ccgcatgtcg gcattactgc tgacaagcag cgcaccgcca aaagcgatcg cggcgatggc
    18601 gaccagatga gccgacgaca cttcaagcag cgccagaatc cccagcacta tgccggccac
    18661 gccagcaaga aacaccatcg acaacccgtt gacggccgat ccgtccaaca tctcggtctc
    18721 gccttcctga cccaggaccc ggcccagatc agacattgtg gacgccccat tcagcaacaa
    18781 ttcgaccccg aacacgatgg tggcgatcgc caccagaaac gttgggctga cacccgccaa
    18841 acccaggatc gccaggatga ccaccgccaa gcccgcgacc gcctcgggaa ccgacccgct
    18901 c
//
LOCUS       DASTNK010000048         6241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_33427, whole genome shotgun sequence.
ACCESSION   DASTNK010000048 DASTNK010000000
VERSION     DASTNK010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33427"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1210)
                     /gene="ruvB"
                     /locus_tag="VFR20_03710"
     CDS             complement(146..1210)
                     /gene="ruvB"
                     /locus_tag="VFR20_03710"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU0229653.1"
                     /translation="MAIHSDSLSGAAAPERIVAPQAASPNEESIERALRPRAFGDYVG
                     QDRVREQLEIFISAARNRQESLDHVLLFGPPGLGKTTLAHIVAHEMGVQLRQTSGPVL
                     ERAGDLAALLTNLEKNDVLFIDEIHRLSPVVEEILYPALEDYQIDILIGEGPSARSVK
                     IDLQPFTLVGATTRAGMLTNPLRDRFGIVARLEFYNTTDLARIVTRSAHLLNADTTAE
                     GAHEIARRARGTPRIANRLLRRVRDYAEVKANGRIDARIANAALSMLEVDPQGLDLMD
                     RKLLDAIVHKFDGGPVGVDSLAAAIGEERDTIEDVIEPYLIQNGYLQRTARGRIATQS
                     TWRHLGLTPPAAPATPDLFE"
     gene            complement(1435..2007)
                     /gene="ruvA"
                     /locus_tag="VFR20_03715"
     CDS             complement(1435..2007)
                     /gene="ruvA"
                     /locus_tag="VFR20_03715"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU0229654.1"
                     /translation="MIGRIRGTLLEKSPPMVCVDVAGVGYEIDVPMSTLYDLPEAGAQ
                     VTLFTHLLVREDAHTLFGFSTAGQRSAFRALIKVTGIGARTALALLSGMTVDELASAV
                     TQQETGRLTRVPGIGKKTAERLLLELRGKFGADMGRHTDAPSSGRDDILNALLSLGYS
                     SSESQAALKNLPQDVDVAEGIRLSLKSLAK"
     gene            complement(2077..2625)
                     /gene="ruvC"
                     /locus_tag="VFR20_03720"
     CDS             complement(2077..2625)
                     /gene="ruvC"
                     /locus_tag="VFR20_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEU0229655.1"
                     /translation="MRILGIDPGLRRTGFGVIDCQGAHLQYVASGTIAVPADQSLAQR
                     LKVILDNVREIVADTHPDVSAIEKVFVNTNPASTLLLGQARGAALCGLADSDLPVHEY
                     TALQIKKAVVGNGHADKTQVQEMVKRLLTLNGTPAADSADALACAICHAHIGPLAQRL
                     QTAAGLPARRGGGRLRGGRLIG"
     gene            complement(2661..4253)
                     /gene="purH"
                     /locus_tag="VFR20_03725"
     CDS             complement(2661..4253)
                     /gene="purH"
                     /locus_tag="VFR20_03725"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU0229656.1"
                     /translation="MTIKTALLSVSDKTGIVDFAKALATRGVRLLSTGGTARLLAEAG
                     LDVTEVAAHTGSPEILDGRVKTLHPKIHGALLARRDSQAHMDTVREQGIELIDLLVVN
                     LYPFRETIARPGCTLADAIENIDIGGPAMLRSAAKNHGSAEGGVTVVIDPGDYERVLA
                     DMDGPGKGPGYGLRLELASKVYAHTAAYDGAIAAYLTSLAEAEPRPGQTPQRNAWPRT
                     LTLQVHQRQALRYGENPHQHAAFYQDLPVRTGLLAGFEQIQGKELSYNNIADADAAWE
                     CARSFDTCACVIVKHANPCGVALAESPEQAYRQALKTDPTSAFGGIIAFNRLVDADTA
                     EAVTSQFLEVLLAPGYTPEALALLTAKKNVRVLSVPPGQSHNEFDVKRVGGGWLVQTP
                     DDHHVPESAFRIVTKKAPTAQQMHDLRLAWRVAKYVKSNAIVFAGNGMTLGVGAGQMS
                     RVDSARIASIKAANAGLSLAGSAAASDAFFPFRDGLDVIADAGATCVIQPGGSVRDDE
                     VIAAADERGVAMVLTGARHFRH"
     gene            complement(4280..4519)
                     /locus_tag="VFR20_03730"
     CDS             complement(4280..4519)
                     /locus_tag="VFR20_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0229657.1"
                     /translation="MSHNNPLEQPVRDSLERYFADLGDAPPHDILGMVFHCVERPVLQ
                     VALEKSGGNQSKAAEMLGITRSTLRKKLIAHDLQP"
     gene            complement(4594..5613)
                     /gene="dusB"
                     /locus_tag="VFR20_03735"
     CDS             complement(4594..5613)
                     /gene="dusB"
                     /locus_tag="VFR20_03735"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU0229658.1"
                     /translation="MRIGPWSLPNPVFVAPMAGVTDRPYRQLCKKLGAGYAVSEMAAS
                     NPRLWDSVKTSRRLNHDGEIDPVAVQIAGSDPAMMAEAAAFNVSRGARIIDINMGCPV
                     KKVCNVAAGSALLRDEDLVARILESVVEACRPLGVPVTLKTRTGWDRESRNAIRIARL
                     AEDSGIAALFLHGRTRCDLYQGVAEYDTIKAVKRAVRIPVVANGDIDSPEKARYVLDY
                     TGADAIMVGRAAQGRPWIFREIVQFLETGAYADPPTYGELRDHLLEHLDDHYRFYGEF
                     IGVRSARKHIGWYLADVPGGDTLVRGINLIDSTCEQTRALERWFSNQPAGQPLPARAD
                     QALAA"
     gene            complement(5719..>6241)
                     /locus_tag="VFR20_03740"
     CDS             complement(5719..>6241)
                     /locus_tag="VFR20_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU0229659.1"
                     /translation="LYGIVWCCDPDHARRLAALTPADFETALHIAFGHRLGHFTLKSD
                     RHVFPLSLHAGPSLLEPHIVAIGNAAQTLHPVAGQGLNLGLRDAAQLAQALTPWLAAS
                     MADPSPILATFARKRRADRYLTAGITDLLPRAFASHNPLVRHACGLGLLALDLCPPLR
                     APLARHLMQGLRT"
BASE COUNT         1015 a         1850 c         2194 g         1182 t
ORIGIN      
        1 ggctcgggtg agcggcgtcg gcgcgcgcaa gctcgatgcc tatggtgcgc agttgctgcg
       61 ttgtgtcggc gcgggcggtg cgtgaaagcg gctgcggtcg atcagcggga tcgctccgcg
      121 attttctcgg gcctcccggg gctgttcatt cgaatagatc gggtgtggca ggtgccgctg
      181 gcggcgtgag cccgaggtgg cgccaggtgg actgggtggc gatgcggccg cgcgcggtgc
      241 gctgcaggta gccgttctgg atgaggtacg gttcgatgac atcttcgatg gtgtcgcgtt
      301 cttcgccgat ggcggcggcc aggctgtcga cccccaccgg gccgccgtcg aatttgtgga
      361 cgatggcatc gagcagcttg cggtccatga gatccagccc ctgcgggtcg acttccagca
      421 tggacaatgc ggcgttggcg atgcgggcgt cgatgcggcc attggccttg acctcggcgt
      481 agtcccgcac gcgtcgcagc aggcggttgg cgatacgcgg tgtgccgcgg gcgcgccgcg
      541 cgatttcatg ggcgccttcg gcggtggtgt cggcgttgag caaatgggcg ctgcgcgtga
      601 cgatgcgcgc gaggtctgtt gtgttgtaga actcgagtcg ggcgacgatg ccgaagcggt
      661 cacgcaaagg gttggtcaac atgccggccc gcgtggtggc gccgaccagg gtgaagggct
      721 gcaggtcgat cttgacgctg cgcgccgacg ggccttcacc gatgaggata tcgatctggt
      781 agtcttccag cgcggggtag aggatttctt cgacgaccgg tgacaaccgg tgtatttcgt
      841 caatgaagag aacgtcgttt ttttcgagat tggtcagcag cgcggcgagg tcgccggcgc
      901 gttccagcac ggggcccgac gtttggcgca attgcacgcc catttcgtgg gcgacgatgt
      961 gggccagggt ggtcttgccc agccccggag ggccgaagag caggacgtga tccaggcttt
     1021 cctggcggtt gcgggccgcc gagatgaaga tttccaattg ttcgcgcaca cggtcttgcc
     1081 cgacgtagtc gccaaaagcc cgggggcgca gcgcccgttc gatggactct tcgttgggcg
     1141 aggcggcttg gggggcgacg atccgttccg gggcggcggc gcctgaaagc gaatcggaat
     1201 gtatggccat ggcgggaatg atgtctggtg gaattgccgg gattttaccg ttcagtggtg
     1261 ggacaccgat gcgttcagtg cttgagtgga ctgctcaagg ccggcgcctg actccgcccg
     1321 gctcgttcca gtattttcac tgtatatgat aacagtgttt gtgtctcgcg cacccaacgg
     1381 taccgtggta atgtcggcgc cgcgtcttgc aacggttcgc acatgcccgc ccgttcactt
     1441 ggccagcgat ttcaggctca accgtatgcc ttccgccacg tccacgtcct gcggcaggtt
     1501 tttcagcgcc gcctgggatt cgctgctgga ataacccagc gacagcaagg cgttcagaat
     1561 gtcgtcgcgc cccgacgatg gcgcgtcggt gtgccgtccc atgtcggcgc cgaacttgcc
     1621 gcgtagttcc agcagcaaac gctcggccgt tttcttgcct atgccgggca cacgtgtgag
     1681 gcgtcctgtt tcctgttggg tgacggcgct cgccagttca tccacagtca tgcccgacaa
     1741 gagcgcgagg gccgttctgg cgccaatgcc tgtgaccttg atcagcgcgc ggaatgccga
     1801 gcgctggccg gccgtggaga agccgaacag cgtgtgcgca tcctcccgca cgagcagatg
     1861 cgtgaacagt gtcacctgcg cgcccgcttc gggcaagtca tacagcgtgc tcatgggcac
     1921 gtcgatttcg tagccaacgc ccgccacatc cacgcagacc atggggggcg acttttccag
     1981 cagagtgccg cgaattcgtc cgatcatcgg gaaaacatcc tgttcagaaa tggattaggg
     2041 tcggatcata ttggaacaga cagcagtcac gaacggtcaa ccaatgagac ggccgccgcg
     2101 caggcgaccg ccgccacgcc tggccggcag gccggcggcc gtttgcaggc gctgggccag
     2161 cggcccgata tgggcatgac aaatggcgca ggccagggcg tcggccgaat ccgcggcggg
     2221 ggtgccgttc agagtcagca agcgcttgac catctcctgc acctgcgtct tgtctgcatg
     2281 accattgccc acgaccgcct ttttgatctg cagggccgtg tattcgtgca cgggcagatc
     2341 gctgtcggcc agcccgcaca gcgccgcgcc acgggcctgg cccagcagca gcgtcgatgc
     2401 gggattggtg ttgacgaaca ccttctctat ggctgacacg tccgggtgcg tgtcggccac
     2461 gatttcccgc acgttgtcga gaatgacctt caggcgttgc gccagcgact gatccgccgg
     2521 cacggcgatg gtgccgctgg cgacatattg caagtgcgca ccctggcagt ctatgacgcc
     2581 gaacccggtc cgtcgcaggc cggggtctat gccgaggata cgcattggct gggacttgtc
     2641 cgttctgaaa gtcgcgtgga tcagtggcgg aagtgccggg cgccggtcag caccatggcc
     2701 acgccgcgct cgtcggcggc ggcgatgact tcgtcgtcgc gcacactgcc accgggctgg
     2761 atgacgcatg tggcgccagc gtcggcgatg acgtccaggc cgtcgcggaa tgggaagaag
     2821 gcgtcggacg ccgccgccga tccggccaga ctgaggccgg cgttggccgc cttgatcgaa
     2881 gcgatgcgcg ccgaatcgac ccggctcatc tggccagcgc ccacgccgag cgtcatgccg
     2941 ttgccggcga acacgatggc gttggatttg acgtatttcg ccacgcgcca tgccaagcgc
     3001 aggtcgtgca tctgttgcgc cgtgggtgcc tttttcgtga cgatgcggaa cgcgctttcg
     3061 gggacgtggt ggtcgtcggg cgtctgcacc agccagccgc caccgacgcg cttgacgtcg
     3121 aactcgttgt gcgactggcc cgggggcacg ctcaggacgc gaacgttctt cttggcggta
     3181 agcaacgcca gggcctcggg cgtgtagccg ggtgccagca ggacttccag gaattggctg
     3241 gtcacggcct cggcggtgtc cgcgtccacc aggcggttga aggcgatgat tccgccgaat
     3301 gccgaggtgg gatcggtttt cagggcttgg cgataggcct gttccggtga ttcggccagg
     3361 gccacgccgc acggattggc gtgtttgacg atgacgcagg cgcaggtgtc gaagctgcgc
     3421 gcgcactccc aggcggcatc ggcatcggcg atgttgttgt atgacagttc tttgccctgg
     3481 atctgttcga agccggcgag caggcccgtg cgcacgggca gatcctgata gaacgccgcg
     3541 tgctggtgcg ggttctcgcc atagcgcaac gcctggcgct gatgcacctg cagagtgagt
     3601 gtgcgtggcc atgcgttgcg ttgcggtgtc tgcccgggcc gcggctcggc ctcggccagg
     3661 ctggtgaggt aggcggcgat cgcaccgtcg taggctgctg tatgcgcgta gaccttgctt
     3721 gccaattcca gccgcaggcc atagccgggg cccttgcctg ggccgtccat gtcggccagc
     3781 acgcgttcat aatcgccggg gtcgatcacc accgtgacgc cgccctcggc cgaaccatgg
     3841 ttcttcgccg cggagcgcag catggccggc ccgccgatat cgatgttctc gatggcatcg
     3901 gccagggtgc atccgggccg cgcgatggtc tcacggaacg ggtagagatt gaccaccagc
     3961 agatcgataa gctctatgcc ttgctcgcgc acggtatcca tgtgcgcctg gctgtcgcgg
     4021 cgggccagca gcgcgccatg gatcttgggg tgcagggtct tgacccgtcc gtcgagtatt
     4081 tccggcgagc cggtatgggc ggcgacctcg gtgacgtcga ggccagcctc ggccagcagg
     4141 cgcgcggtgc ctcccgtgga aagcaggcgc acgccacgcg tcgccagagc cttggcgaag
     4201 tcgacgatgc cggtcttgtc ggaaacggag agtagggcgg ttttgatggt catgggaaac
     4261 gagggtcaga gagggaattt cagggttgca gatcgtgcgc gatcagtttc ttccgcagcg
     4321 tgctgcgggt gatgccgagc atttcggccg ccttggactg gttgccgccg gatttttcca
     4381 gggcgacctg gagaaccggg cgctcgacgc aatgaaacac catgcccagg atgtcgtgcg
     4441 gcggggcgtc gcccagatcg gcgaaatagc gttccagact gtcccggacg ggttgttcaa
     4501 ggggattgtt atggctcatg gcggataagg aaataaatcg aattgagttg ttggtattga
     4561 tatcgttggt gttttgcggc gcaatggggc gaatcaggca gccagcgcct gatcggctct
     4621 ggctggaagc ggctgacccg caggctggtt gctgaaccag cgctccagcg cgcgtgtctg
     4681 ttcacacgtg ctgtcgatca ggttgatgcc gcgcaccaga gtatcacccc ctggcacatc
     4741 ggccagatac cagccaatgt gcttgcgcgc cgatcgcact ccgatgaatt ccccgtagaa
     4801 ccgatagtgg tcgtccaggt gttccagcag atggtcgcgc agttcgccgt aggtgggtgg
     4861 atcggcgtat gcacccgttt ccagaaactg aacgatctcc cggaatatcc agggtcgccc
     4921 ctgggcggcc cggccgacca tgatcgcatc ggcaccagtg taatccagca cgtaacgggc
     4981 cttttcggga gagtcgatat cgccgttggc gacgacggga atacgcacgg cgcgcttgac
     5041 cgccttgatc gtgtcatatt ccgccacgcc ctgatagaga tcgcagcgtg tgcggccatg
     5101 caggaacagg gcggcgatac cgctgtcctc ggccaggcgg gcgatgcgta tcgcgttgcg
     5161 cgattcgcgg tcccagccgg tgcgggtttt cagggtgacc ggcacgccga gcggccggca
     5221 ggcttcgacc acgctttcca gtatgcgggc gaccaggtct tcgtcgcgca gcagggccga
     5281 tccggcggcc acgttgcata ctttcttgac cgggcagccc atgttgatgt cgatgatgcg
     5341 cgcgccgcgg ctgacgttga acgcggcggc ctcggccatc atggccgggt cggagccggc
     5401 tatctgtacg gcgacgggat cgatttcgcc gtcatggttc aggcggcggg atgtcttgac
     5461 gctatcccac agccgtggat tgctggcggc catttccgac acggcatagc ccgcccccag
     5521 cttcttgcag agctgccgat aggggcgatc cgtgacaccc gccatggggg cgacaaagac
     5581 ggggttgggg agggaccagg gaccaatgcg catgccgggg attttaaccc tgtgcgtcgc
     5641 gagcccggga acaaggggct tttgaacaag tcaccgcacc gcctgcatcg gtggaagtgc
     5701 gggcggccgg ttacatggtc aggtgcgcag accttgcatc aggtggcgcg ccagcggggc
     5761 gcgcaagggc ggacacagat cgagcgcgag caggcccagg ccgcaggcgt gccgcaccaa
     5821 cgggttgtgc gaggcgaagg cgcgtggaag caggtcggtg atgcccgccg tcaggtaccg
     5881 atcggcgcgg cgcttgcgag cgaaagtggc cagaatcggg gacgggtccg ccattgaggc
     5941 ggcgagccac ggcgtcagtg cctgggcaag ttgggcggcg tcgcgcagac ccagattcag
     6001 gccttgtccc gccaccgggt gcagggtttg cgccgcgttg ccgatggcca caatatgggg
     6061 ctcgagcagg gacggaccgg catgcaggct caaggggaat acgtggcggt cgcttttgag
     6121 cgtgaagtgg cctagtcgat gcccgaaagc aatgtgcagc gctgtttcga aatcggcggg
     6181 tgtcaaggca gcaagccggc gagcgtgatc ggggtcgcag caccagacga tgccgtacaa
     6241 g
//
LOCUS       DASTNK010000049         3621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1516847, whole genome shotgun sequence.
ACCESSION   DASTNK010000049 DASTNK010000000
VERSION     DASTNK010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516847"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..1214
                     /locus_tag="VFR20_03745"
     CDS             237..1214
                     /locus_tag="VFR20_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEU0229660.1"
                     /translation="MKAIEISEPGGPDVLKLVDRPEPTPGPGEVLIRVSAAGINRPDV
                     FQRQGRYAPPPGASDLPGLEVAGRIVSGDVGDTGLAVGDRVCALVAGGGYAEYCVAPA
                     AQCLPVPQGLTDVEAAGLPETYFTVWSNVFDRGRLSPGESLLVHGGASGIGTTAIQLA
                     SALGHTVYATAGSDDRARAVEKLGAALCINYRTHDYVEEIRRATAGKWVDVVLDMVAG
                     DYINRNIQCLADDGRIVIIALLGGAKATIDCGQILRRRLTITGSTLRPRPVAFKGAIA
                     QSLRRHAWPLLEAGKIRPVIHSEFPLAQARDAHALMDAGDQIGKIILTV"
     gene            1456..2244
                     /gene="tpiA"
                     /locus_tag="VFR20_03750"
     CDS             1456..2244
                     /gene="tpiA"
                     /locus_tag="VFR20_03750"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU0229661.1"
                     /translation="MRTDGRPDDARNPGVNAGKRVRLVMGNWKMNGGLAANATLLSEL
                     VAGAISVPACELAVCAPFPYLAQVRATLDGGPVSWGAQDVSAHEQGAYTGEVSATMLN
                     DFSCRWALVGHSERRAMHGESDQLVADKAAAALAAGITPVVCVGESLGARESGDTNTV
                     ISRQLAPVLALGAQAVARMVLAYEPIWAIGTGRTASPEQAQSVHAHIRGQLSDLGAAQ
                     VRIMYGGSVKAANAASLFAMPDVDGALVGGASLVAEEFLRIAAA"
     gene            2262..2915
                     /gene="secG"
                     /locus_tag="VFR20_03755"
     CDS             2262..2915
                     /gene="secG"
                     /locus_tag="VFR20_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU0229662.1"
                     /translation="MQWLSPTLLAIQVISALVIVVLVLLQQGKGSEMGAAFGSGSAGS
                     LFGASGAANFLSRSTKWAAVIFFVTTGGLAYIAHHPHNRGFDGGVMQGFKSAPAKAPE
                     APAVPAAASSVPSVPTAPAKSGAAGQSSTAPAGSASSVPSAGAAAAPSAGATVDASTA
                     AKSSASDSVPSIGAPAASGKAGAQPSDEAKSASTTSDASHKSGADSAGAKQLDSNKK"
     gene            3044..3128
                     /locus_tag="VFR20_03760"
     tRNA            3044..3128
                     /locus_tag="VFR20_03760"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3078..3080,aa:Leu,seq:gag)
     gene            complement(3367..>3621)
                     /locus_tag="VFR20_03765"
     CDS             complement(3367..>3621)
                     /locus_tag="VFR20_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEU0229663.1"
                     /translation="EEYLVPLGMSVNALSHLLHVPVTRMNDIVRERRGVTPDTALRLA
                     ALFGGDAMSWLNLQQAYDLKKAEAKAARRISEEIRPLEHA"
BASE COUNT          617 a         1136 c         1191 g          677 t
ORIGIN      
        1 gggccaggct attcgtctgt gcggcgcgga ccctgttatt ctttttgttt tcgcggccag
       61 acgacgcccg aacccggaag cccgaaagtg taccccatac tacgctttca ggtgaacggg
      121 cggcgcgccg gcgggtcacc cgacccggcc acgatgggat catcaggacg cgaatctcgc
      181 ggcgcagggc tcgtcatccg gcctctgtgc ctgcctattt ttcacaggag tcctgcatga
      241 aagcgataga aatttccgaa cccggtggcc ccgatgtcct gaagctggtc gatcgtcccg
      301 agcccacccc tgggccaggc gaagtgctca tccgggtgtc ggcggcgggt atcaatcgcc
      361 ccgatgtgtt ccagcgccag ggtcgctacg cgccgccgcc cggagcgtcc gatttgcccg
      421 ggctcgaggt ggccgggcgc atcgtgtccg gcgacgtggg cgataccggg ttggccgtgg
      481 gtgaccgggt ctgcgcgctg gtggccggcg gcggctacgc cgagtactgc gtggcgccgg
      541 cggcgcaatg cctgcccgtg ccccaggggc tcaccgatgt cgaggcggcg gggttgccgg
      601 aaacctattt cacggtctgg agcaatgtgt ttgaccgtgg ccgtctgtcg ccaggtgaat
      661 cgctattggt gcacgggggg gccagcggca tcggcaccac agccatccag ctcgcatcgg
      721 ctctggggca cacggtgtac gccaccgcgg gcagcgacga tcgcgcccgc gcggtggaaa
      781 aactcggcgc ggcgctatgc atcaattacc gcacccacga ctacgtagag gaaatccgcc
      841 gcgccactgc cggcaagtgg gtggacgtgg tgctggacat ggtcgcgggc gactacatca
      901 accgcaacat ccaatgtctg gccgatgacg gccgcatcgt catcatcgcg ctgctgggcg
      961 gggccaaggc caccatcgat tgcggccaga tcctgcggcg ccggctgacc atcacgggtt
     1021 ccacgctgcg cccgcgccct gtggcgttca agggggcaat cgcccagtcg ctgcgccgcc
     1081 atgcgtggcc tctgctggaa gccggcaaga tccgtccggt catccactcc gagttcccgc
     1141 tggcgcaggc gcgcgacgcg catgcgctga tggatgcggg cgatcagatc gggaaaatca
     1201 tcctgacggt ttgaccaggg aagagggcag tgtgtggccg cctcgccgtc ccagggcgtt
     1261 tcgtgacgct tgcagtctgt tgacgcttgc ggccatgcgc gtgcacctcg aacacgctac
     1321 aatgttgggt ttattccaga tcctgataag gctggctgcc cgggggcggc cagcttggcc
     1381 acaaacggcc gcaaatggat tggtggatta gtgccataac ctcaaaccac agacaccgtg
     1441 acagcaagca ccaacatgcg tactgatggg cgtcccgacg acgcgcgcaa ccctggcgtt
     1501 aacgccggca aacgcgtgcg tctggtcatg ggcaactgga aaatgaatgg tggtttggct
     1561 gccaatgcca cgttgttgag tgaactggtt gcgggcgcga tcagcgtgcc ggcctgcgaa
     1621 ctggcggtgt gcgcgccttt tccctatctg gcacaggttc gggcgacgct cgatggtggc
     1681 ccggtttcgt ggggtgcgca agacgtcagt gcgcacgaac aaggcgccta taccggcgag
     1741 gtatccgcca ccatgctgaa cgacttttcg tgccgctggg cgttggttgg ccattccgag
     1801 cgtcgcgcca tgcatggcga atcagaccaa ctcgtggccg acaaggctgc ggcggcgttg
     1861 gctgctggca tcacgccggt ggtctgtgtg ggggaatccc tgggtgcccg cgaatcgggc
     1921 gacaccaaca cggtcatttc ccgccaactg gctccggtgc tcgcgctggg tgcccaggcg
     1981 gtggcgcgca tggtgctggc ctacgagccc atctgggcca taggcaccgg gcgcacggcc
     2041 tcgccggaac aggcgcagtc cgtgcatgcc catatacgcg gtcaattgtc ggatctgggc
     2101 gcggcccagg ttcggatcat gtacggcggc agtgtcaagg cggcgaacgc cgccagccta
     2161 ttcgccatgc ccgatgtcga tggtgccctg gtcgggggcg cctcgttggt ggcggaagaa
     2221 ttcttacgta ttgcggccgc ataggcccgg agtctcagat catgcaatgg ttgtcaccca
     2281 ctcttctcgc gatccaggtc atttccgccc tggtcattgt cgtgctggtt ctgctgcagc
     2341 agggcaaggg ctcggaaatg ggcgcggcat tcggcagcgg ctcggcgggt agtttgttcg
     2401 gcgcctctgg cgcggccaac ttcctgtcgc gatccaccaa atgggccgcc gtcatcttct
     2461 tcgtcaccac cggcggcctg gcgtacatcg cgcatcatcc gcacaatcgc ggttttgacg
     2521 gcggcgtgat gcaaggtttc aaatcggcgc cggccaaggc gcctgaagcc ccggcggtgc
     2581 cggccgccgc gtcgtccgta cccagtgtgc cgaccgcgcc ggccaagagt ggcgccgcgg
     2641 ggcaatcgtc tacggcaccg gcgggttcgg cttccagcgt tccgtccgcg ggggccgccg
     2701 cggctccatc cgccggggcc actgtcgatg cgtccacggc cgcgaaatcg tccgcgtcgg
     2761 attccgtgcc atccataggc gcccccgccg ccagtggcaa ggccggtgcg cagccgagcg
     2821 atgaggcgaa atccgcgtcg accacctccg acgcttccca caagtctggc gccgactccg
     2881 ccggtgcaaa acagctggat tcgaacaaga aatgacaagg ccgcaggcca ggatttcctg
     2941 gcctgcggtt gtgctaaaat ctcgctcttt gctgcggcag ggccttgttt ccgggcgcca
     3001 agggcgcctt ctaataaaaa agcaagaagc caacgcagca catgccgacg tggtggaatt
     3061 ggtagacacg ctatcttgag ggggtagtgg cgaaagctgt acgagttcga gtctcgtcgt
     3121 cggcaccaac tgattaaacg atatcatccg tagtagtaca aaaacccgca tggttactgg
     3181 ccttgcgggt ttttaatttg acacggtacc catatgccgg gttcgtccgc cgggaaatcc
     3241 ttggtgttcc gagacaccag gagcaatgaa cccacctcgg ccgatgccca cacgatggca
     3301 cccggcaagc gtatgcaccg ttgcccgcgg atttatgacg tgcctcaccc ggcgggtgac
     3361 gaaaaatcaa gcgtgttcca gcggccggat ttcctcggag atccgtctgg cggctttcgc
     3421 ctcggccttt ttcaggtcgt aggcttgctg caggttgagc catgacatcg catccccgcc
     3481 aaacagagcg gccaggcgca gggcggtgtc gggtgtgacg ccgcgacgct cgcgaacgat
     3541 atcgttcatg cgggtgacgg gcacgtgcag caaatgggat aaggcgttta cgctcattcc
     3601 caagggaacg agatactctt c
//
LOCUS       DASTNK010000050        29790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_407064, whole genome shotgun sequence.
ACCESSION   DASTNK010000050 DASTNK010000000
VERSION     DASTNK010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 29790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29790
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407064"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFR20_03770"
     CDS             <1..352
                     /locus_tag="VFR20_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229664.1"
                     /translation="LAATVVIAVAVYFVHRYRLIHGKTVNGERILELSGSWGRLALDA
                     NKKQGSLSLRDLSAQFIFADIAGVASVERDGTQAVALSLRHASQPEWHIPLSNGKEAR
                     RWVRILTLAAEQKL"
     gene            486..968
                     /locus_tag="VFR20_03775"
     CDS             486..968
                     /locus_tag="VFR20_03775"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU0229665.1"
                     /translation="MESHLGIDEVARRTGLTAHTLRYYERIGLIAPIARAEGGQRRYT
                     APDLAWIEFLLRLRTTNMPIGTMQVFARLRGAGDSTIPARRQMLEAHLTDVLQAIETM
                     RQSAQALQAKIDHYRSLEQSLAPGSPTDQGHTDEHTGPLRTRARQTTGNRRGSGRAGR
                     "
     gene            889..1284
                     /locus_tag="VFR20_03780"
     CDS             889..1284
                     /locus_tag="VFR20_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0229666.1"
                     /translation="MNTQDRYERGLAKLQEIDGGAGGQVVESLAGIAPDFARYLIEFP
                     FGDIYSRPGLDLKSREIAVVAALTALGNAAPQLRVHIHAALNVGVTREEVIETIMQMA
                     VYAGFPAALNGLAAAREVFAQEDAAAEAL"
     gene            complement(1348..1701)
                     /locus_tag="VFR20_03785"
     CDS             complement(1348..1701)
                     /locus_tag="VFR20_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEU0229667.1"
                     /translation="MSFEGSCHCGAVRFTVDAPVPTQALSCNCSYCRIKGALLAFFPA
                     DQFTLDQGEDTLSSYRFNTHKIEHRFCSKCGSHPFAFGVNPSDGAPMRAINLRCVKAV
                     DLDGLTVQHYDGASS"
     gene            complement(1845..3230)
                     /locus_tag="VFR20_03790"
     CDS             complement(1845..3230)
                     /locus_tag="VFR20_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEU0229668.1"
                     /translation="MTRTQAIASAHQFFDSGRFREILARRVAIPTESQNPERGPVLAD
                     YLEKELRVALEAMGFECRILTHPKGKAPFLFAQRIEDSALPTVLGYGHGDVIRGLDKE
                     WKEGLAPWTLTDADGRWYGRGIADNKGQHSVNLQALQIVLNARGKLGFNAKYIIEMGE
                     ETGSPGLRELCEDNRELFAADLLLASDGPRLSPERPTIFLGSRGSVNFDLSIDARAGG
                     HHSGNWGGLISNPGIQLAHAIASIVSSTGQIRIPEWVPAELPASVRQALADCEVDGGA
                     DGPEIEPWWGEPGLSPAERVFGWCTFEVLAFKTGNPDTPVNAIPPRAWARCQLRFVVG
                     IDPDNIVPALRRHLDRQGLSMVQVVPVREAAFQATRIDPDDPWVKWAEASIANSSGKK
                     PAILPNLGGSLPNDIFTDVLGLRTVWVPHSYPGCSQHAPNEHLPPEILREGLGIMTGL
                     YWDLGEGGTPR"
     gene            complement(3227..4255)
                     /locus_tag="VFR20_03795"
     CDS             complement(3227..4255)
                     /locus_tag="VFR20_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEU0229669.1"
                     /translation="MNAPLTTAMPLGGAGDAADIVLQAHGLTRSFRVGGGMFQPRRTL
                     YAVNGVDLVVDRGDVLAIVGESGCGKSTLARMLLGLLPPSSGRVTLGGQDIGQLDRKK
                     VARRLQPVFQDPYSSLNPRRNIASIVAFPLAIHNIGSPAQRRKQALEMLDLVGLPERY
                     ADRTPSQLSGGQRQRVAIARALVMRPEIVICDEPTSALDVSVQAQILNLLLDLRRELG
                     LTYVFISHNLAVVEHIATRVAVMYMGRVVEQSPTAELFREPRHPYTQALLASVLTPEP
                     SLGIPDIGLGSSFPDPLAPPPGCAFAPRCPRRMPECDARPPSLVASAGRLVACHLYPS
                     QQASGETP"
     gene            complement(4252..5262)
                     /locus_tag="VFR20_03800"
     CDS             complement(4252..5262)
                     /locus_tag="VFR20_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0229670.1"
                     /translation="MNPGTGDVVLDVRDLEVDLETAQGTVRAVRKASFQVRRGETLCL
                     VGESGCGKSMTSLALMGLLPRIARRRAQRITLLGEDLIQASPSRVNALRGDKMAMIFQ
                     EPMTALNPAYTIGNQLMEVHMRHRRGAAAQARDRAVELLGKVGMSSAAQRLGQYPHQL
                     SGGLRQRVMIAMALMCNPELLIADEPSTALDVTIQAQILRLLADLQRELGIAMILITH
                     DLGVVARIAQRVAVMYAGEIVEEADATDLFNMPRHPYTQGLLDCIPVPGRNEPGSRLG
                     TIAGTVPSLLGSFEGCGFRERCAYAQDSCRANVPLRTASGHGWRCVLDALPVKAAKEV
                     PA"
     gene            complement(5284..6147)
                     /locus_tag="VFR20_03805"
     CDS             complement(5284..6147)
                     /locus_tag="VFR20_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0229671.1"
                     /translation="MSTPTAPGFWRRALRNKAFLTGGGLLLLICLIAIFAPWISPHDP
                     YAQDLLHRTIPPAWYAKGSWVHPLGTDPLGRDYLSRLFYGARISLLIGISVALIAGLI
                     GTTMGMTAGYFGGRADMAVSFLVTTRLSMPVILVALATVALVGSSLVVVIAVLGLLKW
                     DRYAVVMRSATQQVCALDYVAAARAAGAGALRILFTEVLPNVVPQLIVVATLEAASAI
                     LLEAALSFLGLGVQPPLPSWGLMISEAKSYMFFSFWLIALPGSALALLIFAINIAGDG
                     LHDLLSPEGRA"
     gene            complement(6144..7067)
                     /locus_tag="VFR20_03810"
     CDS             complement(6144..7067)
                     /locus_tag="VFR20_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0229672.1"
                     /translation="MFAYILKRLGLALLVGLAVSVIAFLLLRLSGDPAIAIAGEGARQ
                     ADIELIRHTYGFDRPIAVQYFDWLGHILRGKFGDSIYFHTPAGPLIFDKLQTTLKLGV
                     AALAVALFISIPLGVLAAIYRNSWVDRLCLAIAVVGQALPNFFFALLLIMVLAVSLHW
                     LPVSGTGTWKNFVMPAISLGYYVAPAFMRLIRAGMVEVLDADYIRTARAKGLAPHSII
                     FKHALRNALVPVVALSAVQLGYLLGGSVVVETIFALDGLGYLAYQSITYKDFPVMQLI
                     VLLLSVIYVVLTLAADIANAWLDPRMRTGGP"
     gene            complement(7117..8658)
                     /locus_tag="VFR20_03815"
     CDS             complement(7117..8658)
                     /locus_tag="VFR20_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0229673.1"
                     /translation="MKILKTASRAWRLALCILMGGLVALSSPAWANKQDDTLRFAYDQ
                     APESVDPYFNNVRIGVIIGANVWDTLIYRDPDTNEYKGLLAKSWKQVDDHTIDFELRQ
                     GVKFHNGEDFDADSVVYTLNFIADPANKVTTQQNVNWIKKAEKLGKYKVRLITKKVFP
                     AALEYLAGPVVIHPAVYYKKVGPKGMNEKPVGTGPYMVSQYTPGKSITLVRNPHYFAD
                     SPKNHAKIGKIEIRFIPDRQTQMAEALSGGADFIMYVPKDQADQAAQMPNLQVVSGET
                     MRISFLQMNIRDGAPSPQLKDIRVRKAIIHAIDRATIAKNIVGAGSRVINTMCFPSQV
                     GCTDDGAPRYTYDPALSKKLLAEAGYPNGFSLDILAYRERSQVEAIIGYLQAVGIRAK
                     LNFLQYAAMRDLNRSNKSSLINQTWGSFSVNDVSASTPVFYGGGPDDVSQDKQVQEWL
                     EKGNNSVALQVRKDAYKKALDRIAEQAYGVPLWSLPVYYVANKDLKFTAYPDELVRFW
                     EMSWK"
     gene            complement(8862..9395)
                     /locus_tag="VFR20_03820"
     CDS             complement(8862..9395)
                     /locus_tag="VFR20_03820"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HEU0229674.1"
                     /translation="MSLTPLTSASTVIQIHAWAAMFALVLGIGVLWRRKGTASHRWSG
                     RVWLVAMLVTVLTSFLIHQLRTWGPWSPIHILSLVTLVLMIRAYVAIRRRNVALHLEL
                     MKATYYNALLLATFFTFLPGRIMHDVVFGASPESSAYATVPLWVWIVGGVIVVGGARH
                     VAAKLGRQAAVGSKSRP"
     gene            complement(9469..10071)
                     /locus_tag="VFR20_03825"
     CDS             complement(9469..10071)
                     /locus_tag="VFR20_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEU0229675.1"
                     /translation="MRHLGYYFWLNSDWAYLGADRLEEVVAKHSVTIHYKPVDLLDVY
                     ARTGGIPLSQRSPQRQAYREQELRRWMARLDIHVNITPKHMCPDATLASCFTIAANAM
                     GLPVGRLYKEILAAQWCHEQNVSDGDVLVGVAQRLGLPGRDILARAGKSAVIELYRRY
                     TDEAVAAGVFGSPSYVYEGELFWGQDRLEFLDAALGGVAR"
     gene            complement(10068..11420)
                     /locus_tag="VFR20_03830"
     CDS             complement(10068..11420)
                     /locus_tag="VFR20_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0229676.1"
                     /translation="MSKSQHADSSAHKRKSRSYVTAGIASMMGTTIEWYDFFLYGTAA
                     ALIFNKIFFPAFDPLMGTLAAFATYSVGFFARPLGGIVFGHFGDRVGRKSMLLITLVM
                     MGVPTILIGLIPSYDSIGYWAAILLVILRFLQGVAVGGEWGGAVLMAVEHAPEGKKGF
                     FGSLPQAGVAPGLLLSSFAMVAVAALPEEAMLSWGWRLPFLASVVLLLIGWFIRVRVA
                     ESPDFEAMQKAGKKAEVPLRIVLKEHPRSVLTVIGARLGEVTWFYTVVTFALAYATGT
                     LGIPKITVLDAIMWGAALSFITMPLCGMLGDRIGHKRVFMLGAVGVVVLGSTFFGLLE
                     TGAPLMITLAMALAIGCVYAALYGPEGSLFSSQFPPEVRYSGISLAVQVSGAVGGGLA
                     PLVATWLLALGGGSPRYVVWYLTGLGAVAFLCAWRMRSENLSREGGHAIRIATDGRFE
                     "
     gene            complement(11579..12346)
                     /locus_tag="VFR20_03835"
     CDS             complement(11579..12346)
                     /locus_tag="VFR20_03835"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEU0229677.1"
                     /translation="MNAPLREKAYFDARATQEMRENLHPVARSVQENLAYACRILAAT
                     GQEAGLAGQISARSQTPGNYWTLRFGLGFDEAAPEDFIEVGPDLETVSGQGMANPATR
                     FHLWVYKARPDVNAIIHAHSPWVSAMVAARQPLVIAQMDMTPFHEDCGFLADWPGVPI
                     ADQEGVLISEALGNKRSIILAHHGYLTAGTTVEEAAYLSVYMERAARMQTRARVYGEL
                     TPVPDHLAREAHDYLLKPSIVKGTFDYWCRQVDKAGT"
     gene            complement(12455..13390)
                     /locus_tag="VFR20_03840"
     CDS             complement(12455..13390)
                     /locus_tag="VFR20_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0229678.1"
                     /translation="MVSVSGETSYPHFQLIDQEQGMDGLDRQVRYFLKIAEMSSLSRA
                     AEELDLSQSGLSRHLAALEAHLGKPLFLRTGRGVTITEAGQKLVHAAGPAFARIDAIL
                     NELRDKEGVTQGSLRIAMIHTVSYYFVSDLLARFVSKYEQVNLSVMARSSPEVVDLVE
                     KGRADIGFVYDSVVASDALESTPLFVDEMCLIARRQDLPDDEPIDLLAANIPLVGFPE
                     HYALRRMLKSAGLDGRVVVVAETVDAMLKLSSTGIGACVLPGQIPSPLLLDYGLVKFR
                     LQSPGLQRRVVAIVRHDTPTGSLARQLLAMTRVSA"
     gene            complement(13464..14108)
                     /locus_tag="VFR20_03845"
     CDS             complement(13464..14108)
                     /locus_tag="VFR20_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0229679.1"
                     /translation="MPGLKCDHLVVIAPDLAEGVAYVRTHLGVDLPYGGRHPEMGTHN
                     HVLRIGEDVFLEVIAVDEDAGRPARARWFGLDDGAAVRRAWDQGLRLRSWVARGTDLN
                     AVLADAPELFDDVVRVSRGDRSWRFCLPRDGSLPARGAAPCVIDWGERGTPVPGMSDS
                     GVALREFILEHPDPDAVRALYRRMGLANPPEVRLGATLRYRACLHTPAGMVQLA"
     gene            complement(14200..15327)
                     /locus_tag="VFR20_03850"
     CDS             complement(14200..15327)
                     /locus_tag="VFR20_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0229680.1"
                     /translation="MQAQPLDRQVEAENAIEVRDLKIHYGAVAAVGPVSFSVPRGKQL
                     TLLGPSGCGKSTTLRAVAGLEKPVAGVIRIGDRNMYSSADNLNVPAEGRGLSMVFQSY
                     AIWPHMTVYDNVAYGLRVRRKSRAEIDAGVKRALSLVQMETYATRPASQLSGGQQQRV
                     ALARACAFSPDVLLFDEPLSNLDAKLRSDMRIELRELQHSLGVTSIYVTHDLEEALAM
                     SDCIVVMNKGRIEQSGTPSEIYNYPRTAFVADFVGSANLITGTVRKDLSANDDVVIET
                     TGGELVHGTSHGRKVDPTATLSVRTVHLQLHAKRPEGNRNVWPVTIARSIFLGDLSEI
                     HVDWGGRRLIIRRSALDRMDEGQQVFLSVDPSRCVLLEHDA"
     gene            complement(15340..17025)
                     /locus_tag="VFR20_03855"
     CDS             complement(15340..17025)
                     /locus_tag="VFR20_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU0229681.1"
                     /translation="MAASVFKSRRSAVSGIAVVVVIGIFVLYPIFFLLQAALKQGDGT
                     VDGSTGYGLGNFARLPSYGSIVWNTLEVSVVATVIAIIVGFLIAYILTRTNVPFRRGL
                     EQLMAVPYYVTPLLGALAWGLLGEPHSGYINMLWRALGGEGAIINVTSAMGIAWVMAL
                     FEGSVAFVMVSAVMQSIDPSLEEASQVIGASRFNTMLRVTLPLVAPGVLGALVYVFAE
                     MLGSFSAALVLGTPSRFYVLTTAIYLMVTQYPPRIPLAAAMGVVLFAVMFLMTYLYRR
                     TIAGRSYVTVSGKAFRPRPIDVGGLRWVYFLLVGAYIFLAVVLPLLTLVYVSLQQLAT
                     ALPSWSNFTLEHYRVALSQNAVRYAIRNSLELGVLTATIGVALTGMISWLLHRTNLPG
                     RAALEYISMFPQAVPRLVFAFGIMWAWLVLPIPVYGTIWLLLIAYVTVFLPLGVRTIS
                     SVLLQLDKTLDECGQVCGANWSQRLRTINIPLLKPGIMAAWMLLFIGSVRELGASILL
                     VGPNSQVLTPAIVDAWNFSSSELTAAMALIQTVVVGVVIAIFTVLMRRRELKG"
     gene            complement(17056..18186)
                     /locus_tag="VFR20_03860"
     CDS             complement(17056..18186)
                     /locus_tag="VFR20_03860"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0229682.1"
                     /translation="MNKEDKAKDGMGSVMIPSRRNMLRAGLSLATLPILSVIAPRGAH
                     AAAKATTAQGFETGADVAQAEKEGALVFYTHDSGAAAAAICEAFHKDFPKITARYVSL
                     QNGSLFSKILAERKASRYEADVIQFSDLGTAVDFQHRGGYEEYHSPQQQYYALDHLSH
                     PAGYYFWIAMGFAGIGYNTDLVKEAEAPAGWKDLLDPKWQGKISVKQANSGMQFTEWY
                     ELRKLYGDGYWKAFAKQKPKGFNSRVQVFDRLGRGDDAVCGMAEWAGYQLAKKKNAPV
                     HFVAPKDGLPSSPMANGVVSKAPHPQAARLFVDWLMSLKGQTFYQDNPYLYYGSIRKD
                     APPMPGGLKLNDFKLLVPTDMKAFLASRKEFNSEWNQMLGLL"
     gene            complement(18248..19981)
                     /gene="araD"
                     /locus_tag="VFR20_03865"
     CDS             complement(18248..19981)
                     /gene="araD"
                     /locus_tag="VFR20_03865"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HEU0229683.1"
                     /translation="MAKNSGRKLRSARWFAPDDLRSFGHRSRMMQLGYSEADFGEKPV
                     IGVLSTWSELNSCHSHFPERVKDVKRGVFQAGGFAVEMPALSVDESFTKPTSMLYRNL
                     LAIETEEIIRSHPLDGVVLMGGCDKTTPGLVMGAISAGVPMIYLPAGPMLRGNYAGTI
                     LGSGSDAWKYWDERRAGNITDEQWLGVQSGIARSAGTCMTMGTASTMTAIAEALGLTL
                     PGASSIPAVDSGHQRMSADCGRRIVEMVREDLTPGKLLTEASVRNAAIVAMALGCSTN
                     AVVHLIAVARRAGLNLTLDDLDALGRETPLIANVRPSGKGYLMEDFHYAGGLRALMKR
                     LEDRLDTSVPTVSGKSLGQTLEGAEVYNHDVIRPLSNPVYQEGSLAVLRGNLCPDGAV
                     IKPAACDPRFRNHEGPALVFDSYPEMKKAIEDESLDVTPDHVLVLRNAGPLGGPGFPE
                     WGMLPIPKALIKQGVRDMLRISDARMSGTSYGACVLHVAPESYIGGPLALLKTGDIVR
                     LDLAGRRLDMLVDDEEIARRRAAWTPPEPRYERGWGWMYSNHITQADKGCDFDFLQTD
                     FGRTPGEPDIF"
     gene            complement(20164..21219)
                     /locus_tag="VFR20_03870"
     CDS             complement(20164..21219)
                     /locus_tag="VFR20_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEU0229684.1"
                     /translation="MATVNHIALSRNVVALLEAAGVDPDKAEVVSDVLLDADLMGHNT
                     HGVALLDSYLDALATGRMRGSGDIKVVSDRGSCVTWDGQRLPGGWLVKKAMALGYERL
                     KQHGVVTFAIGNSHHIGALAVYLRDAAEKGYIAQIKCSTASAARMAPFGGIRPVLTPN
                     PLAEGYPTDGDPVMIDISSSITTTSMTQRLAREGRRYPGQWALTAEGEPTDDPGAVTE
                     GGGTLLPLGGSLKGHKGYGLALGVDLMTQGLAGFGRVDHPDHMALAVFVQLIDPEAFS
                     GLDAFLRQSNHTTRLCRATPAMPGVDTVRVPGDAAARKRRTALEKGLDIPDALLERLE
                     RRARETGAVWQSVDMPV"
     gene            complement(21286..22233)
                     /locus_tag="VFR20_03875"
     CDS             complement(21286..22233)
                     /locus_tag="VFR20_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEU0229685.1"
                     /translation="MTAKPDVMVLYEQRPKAMAQLEEAYTLRRWDQAADKTAFLAEHG
                     AKCRAVATNGHARIDRTMLDALPNLGLVACSSAGFETFDLGLMAERGIALTNTSVALR
                     DDVADTAIMLLLAARRGLVEADAYVRSGDWVRKGMFPLQRALKGSRLGIVGLGMIGTA
                     IAQRAESMGVEVSYWNRSAKDVGWRYRPDVRTLAAESDHLAVTVTGGEGTRGLISRDV
                     IDALGPEGLLINISRGSVVDQDAMVTALREGRLGHAALDVYAQEPTQEAGLVSLRNVT
                     LYPHHASGTVETRDAMAQLVVDNLAAFFAGKPLLTPVRL"
     gene            complement(22294..23814)
                     /locus_tag="VFR20_03880"
     CDS             complement(22294..23814)
                     /locus_tag="VFR20_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HEU0229686.1"
                     /translation="MLNNSFEPHGGHLIAGEWIKTEETFRSAPATGVPHDFSVGTVAL
                     VDQACKAAEEAFRSYGYSSRDERARFLDIIAEEIDGRGAAITKIGCEETGLPAGRLEG
                     ERGRTTGQLRLFAEHIRKGGYLDRRHDKALPDRQPAPRPDMRMMQRPIGPVAVFGASN
                     FPLAFSTAGGDTAAALAAGCPVIVKGHSAHPGTGEIVAEAIHAAVVRCGMPKGVFSLV
                     QGGRRDVGHALVQHPLIKAVGFTGSLGGGRALFDLCAARPEPIPFFGELGSVNPMFLL
                     PEAVAARGAAIAKGWAGSLTLGAGQFCTNPGIAVIREAQAGAFAEASRQALSEVGAQV
                     MLTNGIAEAYRKGRDRIAGGHGVRELLTSTCDLRNASPYLFMVSGADWLANAELAEEV
                     FGPLGLIVTARDDEQMMAIASSIEGQLTCTLHLDAGDSGLGRRLLAILERKAGRVLAN
                     GFPTGVEVCDAMVHGGPYPASTNFGATSVGTLSIRRFLRPVCYQNIPESLLPEDIA"
     gene            complement(23836..24573)
                     /locus_tag="VFR20_03885"
     CDS             complement(23836..24573)
                     /locus_tag="VFR20_03885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0229687.1"
                     /translation="MERTIIVTGASGGIGRATARRFLDEGWRVGLVGRRADALAETAS
                     GNENALVLPCDVTDPQQVDEAFDRVLARWGHLDVLFNNAGVGLKSVLPDDVGVDQWKT
                     VIDVNVTGVFYCARAAFARMRRQTPQGGRIINNGSISAHAPRPGSIAYTASKHAVTGM
                     TRSLSLDGRPFGIACGQIDIGNARTDMAASQAKGVPQADGSIRSEAMMDVEHVASSVF
                     HMAGLPLEANVQFMTVMATTMPCIGRG"
     gene            complement(24607..25506)
                     /locus_tag="VFR20_03890"
     CDS             complement(24607..25506)
                     /locus_tag="VFR20_03890"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0229688.1"
                     /translation="MAYDLDAALAGISGIVVTPYDGKGNVAPERLAPIIDRALGVGVH
                     MPVVNGNTGEFYALTTDEAGVMVREVVAMVGGRAPVLAGVGRGIRDACRLAEMSVEAG
                     ADALMVHQPPDPFVAPRGIVDYVRAIATAGGGLPMMLYLRNDAIGTEGIKALCDLPGV
                     KGVKWATPNPQNLAAAKAACDPSITWVGGLAEVWAPAFYAVGARGFTSGLINVWPERS
                     MAIHAALEAGDYPEANRLIAGMKVFEDIRAAELNGTNVTGVKAALAEQGFDCGSTRPP
                     SAWPLTEAQQRRMRAFLLANGLV"
     gene            25826..26866
                     /locus_tag="VFR20_03895"
     CDS             25826..26866
                     /locus_tag="VFR20_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0229689.1"
                     /translation="MNKSQNPRAKAVGGRPGGRATMAAIGRLAGVSQVTVSRALSDPS
                     KVSPATMQRIQDAIAITGFVPNAVAGALASRRSKLISALVPSLTNLVYASMMQSFSDL
                     MRENGYELLLSECGFQPEAEEKLIAAHLSRRPDAFYLTGIHHTARARQMLIGADIPVV
                     EVWDLTDAPIDICVGFHHREVGWAMADFALEAGYRNAGTVSAGDERALRRRDSFVDRF
                     SARGGTCVGNVSFNGSASVATGRIGLTRLIEEHGFSSGLIACSSDLMAQGILIEAQKR
                     NLSVPGQIAVLGFGDQDFAAFLEPALTTVRVDRPALGTAAAQAVLKRLRNDSPEHSLV
                     DIGFELIRRASA"
     gene            complement(26877..27203)
                     /locus_tag="VFR20_03900"
     CDS             complement(26877..27203)
                     /locus_tag="VFR20_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229690.1"
                     /translation="MKVRDIAHSRTGDKGDTSNISVIAFDAAHYELLRRHLTPDAVKA
                     HFSWIVKGVVTRYELPQLGALNFVMTHTLGGGVTRSLALDAHGKCLSSYMLEFELPEA
                     VAQGGI"
     gene            complement(27206..28537)
                     /locus_tag="VFR20_03905"
     CDS             complement(27206..28537)
                     /locus_tag="VFR20_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEU0229691.1"
                     /translation="MKTVRIGSGAGYSGDRIEPAVELAEKGRLDYLAFECLAERTIAL
                     AQLARKNDPDAGYDPLLEARMRAVLPPCRRNGVTLITNMGAANPRAAAQKTRDVAEEL
                     GLSGLKIAYVTGDDVLEVVKGSSLTILENGESIRTLDGRAISANAYLGVAPLLEALRA
                     GADVIITGRVADPVLFLAPLIHEFGWSLDDWDLLGKGTLVGHLLECAGQVTGGYFADP
                     GVKDVEGLARLGFPLAEVAEDGSAVITKVPGSGGQVTLATCKEQMIYEIHDPISYYTP
                     DVIADFSQVTMAADGRDRVRVAGACGRPRTDTLKVSVGYSDGYIGEGQISYAGPGCRP
                     RGELALEIVEERLRLSSVALSDLRGDLIGLNAIHGGPHSVEPSEVRVRVAARTDSLAE
                     AVRVGNEVETLYTNGPAGGGGVTKSAREVIAVLSVLLPREHIRPAVDYLEV"
     gene            28710..29531
                     /locus_tag="VFR20_03910"
     CDS             28710..29531
                     /locus_tag="VFR20_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0229692.1"
                     /translation="MSDITSRQELFRLDDKVVLVTGCGSIGEGWGNGKAMAVLCAQQG
                     ARVYGVDINLEAAQATHHEIDRRGGTSFVERRDVTAADEVKAMVEACVERFGRIDVLI
                     NNVGRSEPGDPVSMNEDTWAGQLQINLNTAFLTMKQVIPLMAAQGGGSIINISSVAGL
                     RYVGKPQVGYAAAKAALMQMTSTTAVIHAKENVRINCIVPGLMHTPLVARLAEKYAGG
                     DYDGFVQTRNNQVPMGRMGDAWDVAHAALFLASDQSRYITGIELVVDGGLTCATR"
     gene            29639..>29790
                     /locus_tag="VFR20_03915"
     CDS             29639..>29790
                     /locus_tag="VFR20_03915"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0229693.1"
                     /translation="MTQASARHILVDSEEKCKQLKTDIENGADFAAVAKEHSSCPSSR
                     DGGNLGT"
BASE COUNT         5926 a         9621 c         9029 g         5214 t
ORIGIN      
        1 attggccgcc acggtggtca tcgccgtagc cgtctatttt gtgcatcgct atcggctcat
       61 ccacggaaag acagtgaatg gcgagcgcat cctggaactg tctgggagct ggggccgact
      121 ggcactggac gccaacaaga aacagggttc tttaagtctg cgcgacctgt ccgcccagtt
      181 catctttgct gatatcgcgg gggtggcaag cgttgaacgt gacggtacgc aagcggtggc
      241 gctgagcttg cgccatgcct cgcagcccga atggcacatc cccctgtcca acggcaaaga
      301 ggcccgccgc tgggtacgga tcctcacact ggccgcagag cagaagctct gacgcgcgcc
      361 cacggcaaca cctggcaccg aagatccgcg tgttcgccga ttccctcctg gccgaattcg
      421 gccaggaggg aaaaacgaaa taaacccttg acttagagcg cactccaact tttaggatgt
      481 cgtccatgga atcacatctg ggtatcgacg aagtcgccag gcgaacgggc ctgacagccc
      541 acaccctccg gtattacgag cgcatcgggc tcatcgcccc catcgcccgc gccgagggtg
      601 gacaacgtcg ctatacggcg ccggacttgg cctggatcga attcctgcta cggttgcgga
      661 ccacgaacat gcctatcggc accatgcaag tgttcgcaag actacgtggc gcaggggact
      721 ccaccatacc ggcccgccgg cagatgcttg aagcgcactt gacggacgtg ctccaggcta
      781 ttgaaacgat gcgtcaatcg gcccaagcct tgcaggcgaa gatcgatcac tatcgttcac
      841 ttgaacagtc ccttgcgcca ggctcaccca ctgatcaagg acacactgat gaacacacag
      901 gaccgctacg aacgagggct cgccaaacta caggaaatcg acgggggagc gggcgggcag
      961 gtcgttgaaa gcctggccgg aatcgcgccg gatttcgccc gctacctgat tgaattccct
     1021 ttcggggaca tctactcgcg gccggggctc gacctgaaga gccgggagat cgcggtggtt
     1081 gccgcattga cggccctggg aaacgcggcg ccgcagctca gggtgcacat ccacgccgcc
     1141 ctcaacgtgg gcgtgacccg cgaggaagtc atcgaaacca tcatgcaaat ggcggtgtat
     1201 gccgggttcc cggcggcgct caatggcctg gccgcggccc gggaagtctt cgcccaggaa
     1261 gacgcggcgg ccgaggcact ctagggcctt gatgggcagg cccttttgca gtcatgcggg
     1321 gcgactttta gtgtaaacag gccctggtca gctgctggcg ccgtcgtagt gctgtaccgt
     1381 gaggccgtcg agatcgacgg ccttcacgca gcgcaggttg atggcccgca tcggcgcgcc
     1441 atcagagggg ttgacgccaa aggcaaacgg atgggagccg cacttgctgc agaaccggtg
     1501 ttcgatcttg tgcgtgttga agcggtagga agacagggtg tcctcgccct ggtccagcgt
     1561 gaactgatcg gccgggaaaa aagccagcaa cgcccccttg atccggcagt acgaacagtt
     1621 gcagctgagc gcctgcgtgg ggaccggtgc gtcgaccgtg aacctgaccg cgccgcaatg
     1681 acagctaccc tcgaacgaca tggcgatcct cctgggaaaa ccgggtttga ttacgaaatg
     1741 ctgacgaaac accttaaacc ggcatgtcca tcataactgt tacagttatg cgcgtttttc
     1801 cggcccgctg atccgtccgg cgcagccaga agcggtgaag accctcaacg cggcgtgccg
     1861 ccctctccca gatcccagta caggccggtc atgatgccca ggccttcgcg caggatctcg
     1921 ggcggcaggt gttcgttggg cgcgtgctgc gagcaacccg gatacgagtg cggaacccag
     1981 acggtacgca gcccgagcac gtcggtgaag atgtcattgg gcagagagcc tccgagattg
     2041 ggcaggatcg cgggcttctt gccgctggag ttggcgatgg acgcctcggc ccatttcacc
     2101 cagggatcgt ccgggtcgat gcgggtcgct tggaaagcgg cttcgcgcac tggcacgacc
     2161 tgcaccatgg acaaaccctg ccggtccagg tggcgccgca gcgccggcac gatgttgtcg
     2221 gggtcgatgc ccaccacgaa gcgaagctgg cagcgcgccc aggcgcgtgg cgggatggcg
     2281 ttgacggggg tgtcgggatt gccggtcttg aaggccagca cttcgaacgt gcaccagccg
     2341 aacacccgct cggcgggcga caaacccggc tcaccccacc agggttcgat ttccgggccg
     2401 tccgcgccgc cgtcgacctc gcaatcggcc agggcctggc gtaccgaggc gggcagctcc
     2461 gcgggcaccc attcgggaat gcggatctgc ccagtggacg agacgatgct ggcaatggca
     2521 tgcgccagtt gtatgccagg gttggaaatg aggcctcccc agttgcccga atggtggccg
     2581 ccggcacgcg catcgatgct gagatcgaaa ttcacgctcc cgcgcgaacc caggaagatg
     2641 gtgggacgct ccgggctcag gcgcgggccg tccgacgcca gcagcagatc ggccgcgaac
     2701 agttcacggt tgtcttcaca cagttcgcgc aggcccggag agccggtttc ctcgcccatc
     2761 tcgatgatgt acttggcgtt gaaacccagc ttgccgcgtg cgttcagcac gatctgcaag
     2821 gcctgcaggt tcaccgagtg ctggcctttg ttgtcagcga tgccgcggcc gtaccagcgg
     2881 ccatcagcat cggtaagcgt ccagggcgcc agcccttctt tccattcctt gtccaggcca
     2941 cggatgacat cgccatggcc atagccgagc actgtaggca gtgcggaatc ttcgatgcgc
     3001 tgggcaaaca ggaacggcgc cttccccttg gggtgggtca ggatgcggca ttcgaacccc
     3061 atggcctcga gggccacgcg caattccttt tccaggtagt cggccagcac cggtccgcgc
     3121 tcggggttct ggctttcggt gggtatcgca acgcgccgcg ccaggatctc gcggaaacgg
     3181 ccgctgtcaa agaactgatg ggccgaagca atggcctggg tgcgcgtcat ggtgtttctc
     3241 ctgaagcttg ctgcgaggga taaagatggc aggcgaccag ccggccggcg ctggccacca
     3301 gtgacggcgg gcgggcgtcg cattccggca tgcgccgggg gcagcgcggc gcgaaggcac
     3361 agccgggcgg tggcgccagc ggatcgggga aggacgagcc caggccgatg tcgggaatgc
     3421 ccaggctggg ctcgggcgtg agcaccgacg ccagcaatgc ctgagtatac ggatgacgcg
     3481 gctcgcggaa caactcggcc gtggggcttt gttccaccac acgccccatg tacatgaccg
     3541 ccacgcgcgt ggcgatgtgc tcgaccaccg ccaggttgtg gctgatgaac acataggtga
     3601 ggcccagttc gcgacgcagg tccagcagca ggttcagtat ctgcgcctgc accgagacat
     3661 cgagcgccga cgtgggttcg tcgcagatga cgatctcggg ccgcatcacc agggcgcggg
     3721 caatcgccac acgctggcgc tggccgcccg aaagctggct gggagtacgg tcggcatagc
     3781 gttcgggcag gccgacgagg tcaagcattt cgagcgcctg cttcctgcgc tgcgcgggcg
     3841 acccgatgtt gtggatggcc aacggaaacg cgacgatcga tgcgatgttg cgccgtgggt
     3901 tcaacgagga ataagggtcc tggaacacgg gctgcaggcg ccgcgccact tttttgcggt
     3961 ccagctggcc aatgtcctgg ccccccagcg tgacgcgccc cgaactgggc ggcaggagcc
     4021 ccagcagcat gcgcgccagg gtcgacttgc cgcagcccga ttcgcccacg atggccagca
     4081 cgtcaccacg gtccacgacc aggtcgacgc cgttgacggc gtacagggtg cgacgcggct
     4141 ggaacatgcc gccgcccact cggaacgacc gggtcagccc gtgggcctgg agcacgatgt
     4201 cggccgcatc ccccgcgccg ccgagcggca tcgccgtcgt cagaggcgcg ttcatgccgg
     4261 cacctctttg gcggctttga cgggcaaggc atcgaggaca cagcgccagc catggccgga
     4321 cgccgtgcgc agcggcacat tggcacggca tgaatcttgc gcgtaggcgc agcgctcgcg
     4381 gaaaccgcag ccctcgaaag agcccagcag ggagggaacc gtgccggcga tggtgcccag
     4441 ccggctgccg ggctcgttgc gccccggcac gggaatacag tccagcaagc cttgcgtata
     4501 gggatggcgc ggcatgttaa acaaatcggt ggcgtccgcc tcttcgacga tttcaccggc
     4561 atacatgaca gccacgcgct gtgcaatgcg cgccaccacg cccaggtcat gggtgatgag
     4621 gatcatggca atgcccagtt cgcgctgcag gtcggccagc aggcgcagga tctgggcctg
     4681 gatggtcaca tcgagggccg tgctgggctc gtccgcgatg agcagttcgg ggttgcacat
     4741 cagggccatg gcgatcatca cacgctggcg cagcccgccc gaaagttgat gcggatactg
     4801 cccgagccgc tgggccgccg acgacatgcc gaccttgccc agcaattcca cggcgcggtc
     4861 gcgcgcctgg gcggcggcgc cgcggcgatg ccgcatgtgg acttccatga gttgattgcc
     4921 gatcgtgtag gcgggattca acgcggtcat gggctcctgg aagatcatgg ccatcttgtc
     4981 gccacgcaag gcattgacgc gactcgggct ggcctgaatc aggtcttcac caagcaaggt
     5041 gatgcgctgc gcgcgacgcc gggcaatgcg aggcaacaaa cccatgagcg ccagcgacgt
     5101 catggacttg ccgcaaccag actcgccgac caggcacagc gtttcgccgc gccgcacctg
     5161 gaacgatgcc ttgcgcacag cgcgcaccgt gccctgggcc gtttccaggt cgacttccag
     5221 atcgcggacg tcgagcacga catcgccggt tccaggattc atatgatcga tattcatggt
     5281 gcgtcaggcc ctgccttcgg gtgaaagcag atcgtgcagc ccatcgccgg cgatgttgat
     5341 cgcaaagatg aggagcgcca acgcgctgcc cggcagggcg atgagccaga acgagaagaa
     5401 catgtaggat ttggcctcgg agatcatcaa cccccaggat ggcagcgggg gctgcacacc
     5461 caggcccagg aacgacagtg ccgcctcgag caggatggcg ctggcggctt ccagcgtggc
     5521 cacgacgata agctgcggca ccacgttggg cagcacctcg gtgaacagga tgcgcagggc
     5581 gccggccccg gcggcgcgcg ccgccgccac gtaatccagg gcgcagacct gctgcgtggc
     5641 gctgcgcatg accaccgcgt accggtccca tttgagcagg ccgagcacag cgatgaccac
     5701 caccagcgaa ctgcccacca gcgccacggt ggccagcgcc accaggatga ccggcatgga
     5761 caggcgcgtg gtgaccagga acgacacggc catatcggcg cggccgccga aatatcccgc
     5821 cgtcatgccc atggtggttc cgatcagccc agcgatcagt gccacgctga tgccgatcag
     5881 caacgagatg cgcgcgccgt agaacaaccg agacagataa tcgcggccca gcggatcggt
     5941 gcccagcgga tgcacccaac tgcccttggc ataccaagcg ggggggatcg tgcgatgcag
     6001 aaggtcctgc gcgtaggggt cgtgcggcga aatccatggc gcgaagatgg cgatcaggca
     6061 gataagcagc agcaggccgc ccccggtgag gaaggctttg ttgcgcaggg cccggcgcca
     6121 gaacccggga gcggtgggcg tgctcatggg ccccccgtgc gcatgcgcgg atccagccag
     6181 gcattggcga tatcggcggc cagggtcagc acgacgtaaa tcaccgacaa cagcagcacg
     6241 atgagctgca tgacggggaa atccttgtag gtgatgctct ggtaagccag gtagcccagg
     6301 ccgtcgaggg cgaatatggt ttccaccacc accgagccgc ccagcaggta gcccagttgc
     6361 accgcgctca gcgcgaccac cggcaccagc gcattgcgca gcgcatgctt gaagatgatg
     6421 ctgtgcggcg cgaggccctt ggcgcgcgcg gtgcggatgt agtcagcgtc cagcacttcg
     6481 accatgcccg cgcggatcag ccgcatgaac gccggcgcca cgtagtagcc cagcgatatg
     6541 gccggcatga cgaagttctt ccaggtaccg gtaccggaca ccggcaacca atgcagcgag
     6601 accgccaaca ccatgatgag caacagcgcg aaaaaaaagt tgggcaacgc ctggcccacc
     6661 accgcgatag ccaggcacag gcgatcgacc cagctattgc gatagatggc cgccagcaca
     6721 cccagcggaa tcgagatgaa caacgcgacc gccagcgccg ccacgccgag cttcagcgtg
     6781 gtctgcagct tgtcgaaaat gagcgggccg gcgggcgtgt gaaaatagat ggaatcgccg
     6841 aacttcccgc gcagaatgtg gcccagccag tcgaagtact gcacggcaat ggggcgatca
     6901 aagccgtagg tgtggcggat gagctcaatg tcggcctggc gcgcgccctc gcccgcgatg
     6961 gcgatggccg ggtcgcccga aaggcgcaac agcagaaagg cgatcaccga aacggccaac
     7021 ccgaccagca gcgcaaggcc cagccgcttc agtatgtagg caaacatcta ttcgtctcca
     7081 ggactgccac gccgccaagc ggcgtggcac gcgccatcac ttccagctca tttcccagaa
     7141 acggaccagt tcgtcggggt aggccgtgaa tttcaagtct ttgttcgcca cgtagtacac
     7201 gggcaacgac cacaacggca caccgtaggc ctgctcggcg atgcgatcga gcgccttctt
     7261 gtaggcgtcc ttgcgcactt gcagcgccac cgaattattg cctttctcga gccattcctg
     7321 tacctgcttg tcctgcgaga catcgtcggg gccgccaccg tagaacaccg gcgtggaagc
     7381 cgacacatcg ttgaccgaga acgagcccca ggtctggttg atgagtgacg acttgttcga
     7441 gcggttcagg tcgcgcatgg cggcgtattg caggaaattc aacttggccc tgatgcccac
     7501 ggcctgcaga tagccgatga tggcttccac ctggctgcgc tcgcggtaag ccaggatatc
     7561 caggctgaag ccgttgggat agcccgcctc ggccagcagt ttcttggaca gagcggggtc
     7621 gtaggtgtaa cgcggcgcgc cgtcatcggt gcaaccgacc tgggacggaa agcacatggt
     7681 gttgatcacg cgcgagcccg cgcccacgat gttcttggcg atggtggcgc ggtctatggc
     7741 gtggatgatg gccttgcgta cgcggatgtc cttgagctgc gggctgggcg cgccgtcccg
     7801 gatgttcatc tgcaggaacg agatgcgcat ggtttcgcca ctgaccacct gcaggttggg
     7861 catctgggcg gcttggtcgg cctgatcctt gggcacgtac atgatgaaat ctgccccccc
     7921 tgacaaggcc tcggccattt gtgtctggcg atcgggaatg aaacggatct cgatcttgcc
     7981 gatcttggcg tggttcttgg gcgaatcagc gaaataatgc ggattgcgca ccagggtgat
     8041 cgacttgccg ggtgtgtatt gcgacaccat gtaggggccc gtgccaacgg gcttttcgtt
     8101 catgcccttg gggccgactt tcttgtaata cacggcgggg tggatgacga cgggcccggc
     8161 caggtattcc aacgcggcgg ggaacacctt cttggtgatg agccgcacct tgtacttgcc
     8221 gagtttttca gccttcttga tccagttgac gttctgctgg gtcgtgacct tgttggccgg
     8281 atcggcgatg aagttcaatg tgtagaccac cgagtcggca tcgaagtctt caccattgtg
     8341 gaacttgacg ccctggcgca attcgaagtc tatggtgtgg tcatcgacct gcttccagct
     8401 tttggcgagc aaacccttgt attcattggt gtcgggatcg cggtagatga gcgtgtccca
     8461 gacgttggcg ccaatgatga cgccaattcg aacattgttg aaatacgggt cgacgctttc
     8521 cggggcctga tcgtaggcga aacgcagggt gtcgtcctgc ttgttggccc aggccgggct
     8581 gctgagggcg accaggccgc ccatcagaat acacagagcc agccgccaag cgcggcttgc
     8641 ggttttcaga attttcatgc cttcctcttc ttcgctgggg taacagggaa ccgtgtcttg
     8701 ccgcacggct ttttggtctg accacctgag cctcgaagtt tacagggatg ggacagcgcc
     8761 tggggcgcgc ctgcctcgtg gtttaccctg aaccatggcg caacggcccg gtggatgcgc
     8821 tcgtcgccgg ggccggggcc ggcaagtggg atcgcgtatt gtcagggtcg gcttttcgac
     8881 cccacggccg cctgcctacc cagtttcgcc gcgacatgcc tggcaccgcc caccacgatc
     8941 acgccaccca cgatccacac ccacaacggc accgtcgcat acgccgacga ttcaggcgat
     9001 gcgccgaaca cgacgtcatg catgatgcgg ccgggcagaa aggtgaagaa cgtggccagc
     9061 agcagcgcgt tgtaataggt ggccttcatc agttccaaat gaagggcgac attgcgccgc
     9121 cgaatggcca cgtacgcgcg aatcatcagc accagggtga cgagcgacag gatatgaatc
     9181 ggactccatg gcccccaggt ccgcagttga tggatgagga acgaagtaag caccgtgacc
     9241 agcatggcca ccagccagac acgccccgac caacgatggc tggccgtgcc cttgcgccgc
     9301 cacagcacgc caatccccag aaccaaggcg aacatggccg cccaggcgtg tatctggatg
     9361 acggtggaag cggaggtcag gggtgtcagg ctcatgaccg agcacgttaa gtgatccctg
     9421 ccaaagcgac aatcgagaaa tacccggcag ccacttcgtc gctccggttc aacgtgcgac
     9481 gccccccagc gcggcatcca gaaactccag cctatcctgc ccccagaaga gctccccctc
     9541 atagacatag ctcggcgaac caaacacccc tgcggccacc gcctcatcgg tataacgccg
     9601 gtacagttct atcaccgccg acttgccggc gcgcgccaaa atatcccggc ccggcaaccc
     9661 cagccgctgc gccaccccaa cgagcacgtc gccatccgag acattctgtt catgacacca
     9721 ctgtgctgcc agaatctctt tgtatagccg ccccacgggc aatcccatgg cgttcgcggc
     9781 gatcgtgaaa caggatgcaa gtgtggcgtc cgggcacata tgcttgggtg tgatgttgac
     9841 atgaatgtcg aggcgagcca tccaccgccg cagctcctgc tcccggtagg cctgccgttg
     9901 cggggaacgc tgtgacagcg ggatgccgcc agtgcgcgca tagacgtcga gcaggtccac
     9961 gggcttatag tggatggtga cgctatgctt ggcaacgacc tcctcgagac gatccgcccc
    10021 cagataagcc caatccgagt tcaaccagaa ataatagccg aggtgcctca ttcgaaccgc
    10081 ccgtcagtcg cgatcctgat tgcgtgtccg ccctcccttg acaggttttc cgaccgcatg
    10141 cgccacgcgc agaggaaggc gacagcacca agcccagtca agtaccagac gacataacgc
    10201 ggactacctc caccaagcgc gagcagccac gtcgccacga gcggagcgag gccgccgccc
    10261 accgcacccg acacctgaac agccaatgag atgccgctat agcgcacttc gggtggaaac
    10321 tgactggaga acaggcttcc ttccgggccg tacagcgctg catagacgca gcctatggcc
    10381 aacgccatgg ccaacgtgat catcaagggc gcgccagtct caagcaagcc aaagaatgtg
    10441 cttcccaaca ccacgacacc aacggcaccg agcatgaaca cgcgcttgtg cccgatgcga
    10501 tcacccagca tgccacacaa cggcatggta atgaatgaca aggctgcgcc ccacatgatg
    10561 gcgtccagca ccgtgatttt cggaatgccc agcgtgcccg tggcataggc cagcgcaaaa
    10621 gtgacaacgg tatagaacca ggtgacttca cccaggcggg cgccgatcac agtcaagaca
    10681 ctgcgcggat gctccttgag cacgatgcgc aacggcacct cggccttttt gcccgccttc
    10741 tgcatggcct cgaaatccgg cgactccgcc acgcgaacac ggatgaacca accgatcaga
    10801 agcagtacga cactggccag gaaaggcagt cgccaccccc agctcaacat ggcctcctct
    10861 ggcaaagccg ccaccgccac catggcgaac gaggacagaa gcaatccagg ggccacccca
    10921 gcctgcggca gactgccgaa gaagcctttc ttgccttcgg gcgcatgctc gacagccatc
    10981 agcaccgcac cgccccactc gccgcccact gcaacgccct gcaagaagcg caggatcacg
    11041 agcagtatgg ccgcccaata gccgatgctg tcgtacgaag ggatcaggcc aatcaggatg
    11101 gtgggcacgc ccatcatgac cagcgtgatg agcagcatgg acttgcggcc cacccggtca
    11161 ccaaagtggc caaacacaat gccacccagc ggccgcgcga agaacccaac ggagtaggtc
    11221 gcgaaggcgg ccaacgtgcc catgagcggg tcgaaggccg ggaagaagat tttgttgaag
    11281 atcagcgctg ccgcggtgcc gtatagaaaa aagtcatacc actcgatggt ggtgcccatc
    11341 atgctggcga tgcccgcagt gacgtagctt ctggacttgc gtttgtgcgc ggacgagtcc
    11401 gcgtgctgag atttggacat gattgcctcc ctgccggtga aagccggcat tgcgtgtatt
    11461 tagaattttg gttgcgggtg ggcctggccc gacaccgcga cggccatcca gccctaccca
    11521 ggccggacgc cacgcccccc tggacaccag gggcggtttc caaccaaaga atagttggtt
    11581 aggtgcccgc cttatccacc tggcggcacc aatagtcaaa ggtccccttg acgatagatg
    11641 gtttaagcag gtagtcgtgc gcttcccgag ctagatgatc cggaaccggg gtgagctcgc
    11701 catagacgcg cgcgcgcgtc tgcatgcgcg cggcacgttc catgtatacc gacaagtagg
    11761 cggcttcctc cactgtggtg ccagccgtga gataaccatg atgggcaagg atgatggagc
    11821 gcttgttacc caacgcctcg gaaatcagaa cgccttcctg gtccgcgata ggcacgccgg
    11881 gccaatccgc caggaacccg caatcttcat ggaagggcgt catatccatc tgggcgatga
    11941 ccagcggctg gcgtgcagcg accatggcgc ttacccaagg ggaatgggca tggataatgg
    12001 cattgacgtc ggggcgcgcc ttgtacaccc acaagtggaa acgcgtggcg gggttggcca
    12061 tgccctggcc agacacggtt tccaggtcgg gcccgacctc aatgaagtcc tctggcgcgg
    12121 cctcgtcgaa accgaggccg aaacgcagtg tccaatagtt cccgggcgtc tgcgagcgcg
    12181 cgctgatctg tcccgcgagc cccgcttcct ggcccgtcgc ggccaggata cggcaggcat
    12241 aagccaggtt ttcctgaacg ctacgggcaa ccggatgcag gttttcgcgc atctcttgtg
    12301 tggcgcgcgc gtcgaaatat gctttttccc tcaatggtgc attcatggct cgtctcttca
    12361 cttccgtaat ttgaaatggg cgcgggaagc cccggcgcga aaaccattct aggaacggga
    12421 gcacgaaaga gccatgaact tcaggtcata acagctatgc agagacgcgc gtcatggcaa
    12481 gcagttggcg cgccagcgag cccgtgggtg tgtcgtggcg cacgatggcc acgaccctgc
    12541 gctggagccc cggggactga agacggaatt tgaccaaccc atagtcgagc aacagcggcg
    12601 aaggtatctg cccgggcagc acgcaggcgc cgatgccagt ggaactgagc ttcagcatcg
    12661 catcgacggt ttctgccacg accaccacgc ggccatccag gcctgcgctt ttcagcatgc
    12721 ggcgcagggc atagtgctca gggaagccaa ccaggggaat gttcgcggcc aggagatcaa
    12781 tcggctcatc gtccggcaag tcctgccggc gcgctatcag gcacatctcg tccacgaaca
    12841 gaggcgtcga ctccagcgca tcggacgcca ccaccgagtc gtatacgaag ccgatgtccg
    12901 cccggccctt ttccaccaga tccacgacct cgggagaact gcgcgccatc acggagagat
    12961 tgacctgctc gtatttgctg acgaaacgcg ccagaagatc ggacacaaag tagtagctga
    13021 cggtgtggat catcgcgatg cgcaggcttc cctgcgtgac cccttccttg tcgcgcagtt
    13081 cattcaagat tgcgtcgata cgggcaaagg ccggccctgc ggcatggaca agtttctggc
    13141 cggcctcggt gatcgtcacc cctctgccgg tacgcaggaa caagggcttg cccaggtgcg
    13201 cctccagtgc ggccaagtgc cggctcaggc ccgactggct caggtccagt tcctcggccg
    13261 ctcgcgacag cgaggacatc tcggcaatct tgaggaaata ccgaacctgc cggtccaggc
    13321 cgtccatgcc ttgctcctgg tcgatcaatt ggaaatgcgg gtaactggtt tccccagaca
    13381 cggaaaccac acgtgtcgcc ctttgcaaac gggccgcttg cttcgatcgg gtactttact
    13441 ctacgaggaa agtcgatccc ctctcaggca agctgaacca tgcctgctgg cgtatgcagg
    13501 cacgcccggt atcgcagtgt cgcgcccagt cgaacttctg gcggattcgc caagcccatg
    13561 cgccgataca atgcacgcac cgcgtccgga tctggatgct ccaagatgaa ttcgcgcaac
    13621 gccacgcctg agtccgacat gccaggaacc ggggtgccgc gttcccccca gtcaatcacg
    13681 cacggggccg cccccctcgc gggcaacgac ccatcgcgcg gcaggcagaa acgccacgag
    13741 cgatcacccc gtgacacacg aacgacatcg tcgaatagtt caggcgcgtc agccaacacg
    13801 gcgttcagat ccgtgccgcg cgccacccag cttcgcaacc gcagaccctg atcccaggct
    13861 cgccgcacag cggcaccatc gtccaggccg aaccagcggg cgcgcgctgg tcggcccgcg
    13921 tcctcatcga cagcgatcac ttccaggaag acatcttccc cgatccgcag cacatgattg
    13981 tgcgtgccca tttccgggtg gcgccccccg tagggcagat cgacgcccag gtgggtgcgg
    14041 acgtacgcaa ccccctcggc tagatccggc gctatgacga ccaggtgatc gcatttaagt
    14101 ccgggcatgg tgttctctct ttctttctga aaacgcaaca cccgaatggt acgccgacgg
    14161 cggaagccgc gctgcagcgc acccgcaacc cgcatctctc tatgcgtcgt gctccagcag
    14221 gacgcaacgg cttgggtcta ccgacaggaa cacctgttgg ccttcatcca tccgatcaag
    14281 cgccgaacgc ctgatgatca aacgccggcc accccagtcg acgtggattt cgctcaggtc
    14341 gccgaggaag atcgaccggg cgatggtcac cggccataca ttacgattcc cctcaggccg
    14401 cttggcatgc agttgcagat gcaccgtgcg gaccgacagc gtcgccgtgg ggtccacctt
    14461 gcgcccatgg gacgttccat ggacaagttc gccgcccgtg gtttcgatca ccacgtcgtc
    14521 attggcggac aggtctttgc gcaccgtccc ggtgatgaga ttggcggaac cgacgaagtc
    14581 cgcgacaaag gccgtccgcg ggtagttgta tatctcggac ggggtgccgg attgttcgat
    14641 ccggcccttg ttcatcacca cgatgcaatc cgacatggcc agcgcctctt caagatcgtg
    14701 ggtcacatat atcgaggtca cgccaaggct gtgttgcaat tcccgcaatt caatccgcat
    14761 gtcgctgcgc aatttggcat cgagattcga aagcggctcg tcgaagagca ggacatccgg
    14821 ggaaaatgcg caggcgcgcg ccaaggccac ccgctgctgc tggccgcctg aaagctgcga
    14881 cgccggccgc gtcgcatatg tttccatctg gaccagggaa agcgcacgct tgacgccggc
    14941 atcgatctct gcgcggctct tgcgacgcac tctcaggcca taggccacgt tatcgtagac
    15001 cgtcatgtgc ggccagatgg cgtatgactg gaacaccatg gacagtccgc gaccttcggc
    15061 cggtacgttc agattgtccg ctgaggaata catgttgcga tcgccgatcc tgatcacccc
    15121 cgccaccggt ttttccagcc ccgcgacggc gcgcaaggtc gtggacttcc cgcaacccga
    15181 tgggccaagc aaggttaact gcttgcccct cggcacggaa aagctgacgg ggccgactgc
    15241 ggccacggcg ccataatgga ttttcaggtc ccgcacttcg atggcgttct cggcctcgac
    15301 ttgccgatct agtggttgtg cttgcattcg catcgaaaat caccctttca attcgcgccg
    15361 acgcatcagc accgtgaata ttgctatgac gacaccgacg accacggtct ggatcagcgc
    15421 catggcggcg gtcagttcgg aactgctgaa gttccatgcg tcgacgatcg ccggcgtcag
    15481 gacttgcgag ttcgggccga cgagcagtat cgacgccccg agttcgcgca cggacccgat
    15541 gaacagcagc atccaagcag ccatgatgcc gggcttcaac agtgggatat tgatcgtgcg
    15601 caaccgctgg ctccagtttg cgccgcagac ctggccgcac tcatcgagcg tcttgtcgag
    15661 ctgcagcagc acactggaaa tggtgcgcac ccccagaggc aggaacaccg tgacataggc
    15721 gatcaacagc agccagatcg tgccgtagac cgggatgggc agcaccagcc atgcccacat
    15781 gatgccgaag gcaaagacca ggcgcggcac cgcttgcggg aacatcgata tgtattcgag
    15841 cgcggcccgg cctggcagat tggtgcgatg caggagccag gaaatcattc ccgtcagggc
    15901 aacaccgatc gtcgccgtca gcacgccaag ctccagactg ttgcgtatgg catagcgcac
    15961 cgcattctgc gacagcgcga cccgataatg ctcaagcgtg aaattcgacc agctcggcag
    16021 agccgtcgca agctgctgca gcgatacata aaccagcgtc agcagcggca ggacgaccgc
    16081 caggaagata taagcgccaa caagcaggaa atagacccaa cgcaagccgc ccacatcgat
    16141 cgggcgcggc cggaaagcct tgccgctgac ggtgacgtag ctgcggcccg cgatcgtccg
    16201 acggtacaga taggtcatca gaaacatgac cgcaaacagc acgacgccca tggccgccgc
    16261 cagcggaatg cggggcgggt attgggtcac catcaggtag attgccgtcg tcaggacgta
    16321 gaaccggctt ggcgttccaa gaaccagcgc cgcggagaac gatccgagca tctcggcgaa
    16381 gacatagacc agggcgccga gcacccccgg cgcgaccagt ggaagcgtga cccgcagcat
    16441 cgtgttgaag cggctggcgc cgatgacttg cgaggcctct tccagggaag ggtcgatcga
    16501 ctgcatgacc gcgctgacca tgacgaaggc gacggagcct tcgaacaacg ccatgaccca
    16561 ggcgatgccc atggcactcg tgacattgat gatcgccccc tcaccgccca gcgcccgcca
    16621 caacatgttg atatagccgc tgtgcggttc gcccagcaga ccccaggcga gcgcgccgag
    16681 cagcggtgtc acgtaatagg ggaccgccat gagctgttca agcccgcggc gaaacggcac
    16741 gttcgtgcgc gtcaggatat aggcaatgag gaagccgacg atgatcgcga tgacggtcgc
    16801 aaccaccgag acttccagcg tattccagac aatactgccg tagctcggca gccgcgcgaa
    16861 gttccccagg ccgtagccgg tactgccgtc gaccgtgccg tccccctgct tcaaggcggc
    16921 ctgaagcagg aagaagatcg ggtacagaac gaaaatcccg atcaccacga cgacggcgat
    16981 tccggagact gcacttcttc gtgacttgaa aacactggcc gccatgcggt tcactcctgg
    17041 tcaggggtaa ggggatcaga gtagccccag catctggttc cattcggaat tgaattcctt
    17101 gcggcttgcc aggaacgcct tcatgtcggt cgggaccagc agcttgaaat cgttgagctt
    17161 gagcccccca ggcatgggcg gcgcatcttt gcggatcgaa ccgtagtaca ggtaagggtt
    17221 gtcctggtag aaagtctggc cttttagcga catcagccag tcgacgaaca accgcgcggc
    17281 ctgcgggtgc ggcgccttgc tgacgacacc gttggccatg ggcgaggacg gcagaccgtc
    17341 tttcggcgcg acgaaatgga cgggcgcgtt cttcttcttg gcaagctggt agccagccca
    17401 ttcggccatg ccacagaccg catcgtcgcc ccgtcccaga cggtcgaaga cttgcacccg
    17461 ggaattgaag cctttcggct tctgcttcgc gaacgccttc cagtagccat cgccgtacag
    17521 cttgcgcaat tcgtaccatt ctgtgaactg catgccagaa ttggcctgct tgacgctgat
    17581 cttgccctgc catttcgggt cgagcaagtc tttccagcca gccggcgctt cggcttcctt
    17641 gaccaggtcg gtgttatatc cgatgcccgc gaaacccatg gcaatccaga aatagtatcc
    17701 cgcgggatga ctcaggtggt cgagcgcata gtactgttgc tgcggtgaat gatattcctc
    17761 gtagccgccc ctgtgctgga agtcgacggc ggtgcccagg tcagagaact ggatgacatc
    17821 cgcttcgtaa cgggacgcct tgcgctcagc aagaatcttg ctgaacagcg acccgttctg
    17881 caggctgaca tagcgcgccg ttatcttcgg gaagtctttg tggaacgctt cgcaaatcgc
    17941 cgcagcggcg gcaccgctgt catgcgtata gaaaaccagg gcgccttcct tttcggcctg
    18001 cgcgacatcg gcgccggttt caaagccctg ggcggtcgtt gccttggctg ccgcatgagc
    18061 gccacgaggc gcgatcacgc tcaggatcgg gagtgtcgcc agtgacagcc ccgcccgtag
    18121 catgttgcga cggctcggaa tcatcacact gcccattcca tcttttgcct tgtcttcttt
    18181 gttcatgcct tcctccttgc tggtttccgt acttgcggtg aattttgcag ccatgccctg
    18241 ctcagtttca gaaaatatcg ggttccccgg gcgtgcgccc gaagtcggtc tgcaggaaat
    18301 caaagtcaca gcccttgtcg gcctgggtga tatggttgga gtacatccag ccccagccac
    18361 gctcgtagcg tggttctggc ggggtccatg ccgcgcggcg ccgggcgatc tcttcgtcgt
    18421 cgaccagcat gtccaggcgt cttcccgcca ggtcgagacg cacgatgtcg ccggttttga
    18481 gtaacgcaag cgggcccccg atatagctct caggtgccac atgcagcaca caggcgccat
    18541 aggacgtacc cgacatgcgc gcatcggaaa tcctcagcat gtctcggacg ccctgcttga
    18601 tcagcgcttt cggaatcggc agcatgcccc attccggaaa acccgggccg cccagcgggc
    18661 cagcgttgcg caggacgagc acatggtcgg gcgtgacatc gaggctctcg tcctcgatcg
    18721 ccttcttcat ctcgggatag ctgtcgaaga ccagcgcggg gccttcatga ttgcggaatc
    18781 tgggatcgca ggcggccggc ttgatcaccg ccccatccgg gcagagattg ccgcgcagaa
    18841 ccgccagcga cccttcttga tagacggggt tcgaaagcgg ccgaatcacg tcgtggttgt
    18901 agacttccgc accttccagg gtttgcccaa ggctcttgcc ggaaaccgtc ggcaccgagg
    18961 tgtcgagcct gtcttcgagc cgcttcatca gcgcccgcag ccctccggca tagtgaaaat
    19021 cttccatcag gtagcctttg ccagaggggc ggacattggc gatcagcggc gtctcgcggc
    19081 cgagcgcgtc gagatcgtcg agcgtgaggt tcaagcccgc ccgccgcgcc acggcgatga
    19141 gatgcacgac cgcgttggtc gaacagccga gcgccatggc gacgatcgcc gcgttcctga
    19201 ccgaggcttc ggtcaggagc ttgcctggcg tcagatcttc ccggaccatc tccacaatgc
    19261 gccggccgca gtcggcggac atgcgctgat ggcccgagtc aaccgcggga atggacgaag
    19321 cgccgggcaa cgtcagcccc agggcttcgg cgatcgccgt catcgtcgac gcggtgccca
    19381 tcgtcatgca ggtgcccgcc gagcgcgcga tgccgctctg tacccccagc cactgctcgt
    19441 ccgtgatgtt gccggcacgg cgctcgtccc agtatttcca ggcgtccgag cccgagccga
    19501 ggatcgtacc ggcataattg cccctgagca tcgggccggc gggcaggtag atcatcggca
    19561 cgccggccga aatcgcgccc atcaccagac cgggcgtcgt cttgtcgcag ccccccatca
    19621 gcaccacgcc gtccagcgga tgcgagcgga tgatttcctc tgtttcgatc gccagcagat
    19681 tgcggtaaag catggaggtc ggcttggtga agctttcgtc caccgagagc gccggcatct
    19741 ccacagcgaa ccccccagcc tggaacaccc cacgcttgac gtccttcacg cgttcaggga
    19801 agtgcgaatg gcagctgttg agctccgacc aggtgctcag caccccgatc accggctttt
    19861 caccgaaatc cgcttccgaa tagccgagtt gcatcattcg ggagcgatgc ccaaagctgc
    19921 gcaaatcgtc cggcgcaaac cagcgcgcgc ttctcaattt tcttccggaa ttctttgcca
    19981 tttcatcagc cctggagcat ccttatttct ttaagtatgc gcatacataa aataaataac
    20041 aattaggtaa ttacccgcat taatgcgata tatatttgtt tttattgagt taattcagga
    20101 atgtttgaat aaaaaattac gtatgcgcaa tcatcaaaac aagatgattc tcttgttgca
    20161 gtatcagacc ggcatgtcca cgctttgcca gacggcccct gtctcgcggg cgcggcgctc
    20221 gagccgctcc aacagggcat cgggaatgtc cagacccttt tcaagcgccg tacgccgctt
    20281 gcgtgcggcg gcatcgccag gcacccgcac ggtgtcaacg cccggcatcg ccggcgtggc
    20341 ccggcacaat cgcgtggtgt ggtttgactg gcggaggaat gcgtcgagcc cggagaaggc
    20401 ttctgggtcg atgagctgga caaaaacggc aagggccatg tgatccgggt gatccactct
    20461 tccgaatccg gcgaggccct gcgtcatgag gtcgacgcca agcgccaacc cgtatccctt
    20521 gtgtcctttc agactgccgc ccagcggcag cagcgtgccg ccgccttccg tgacggcgcc
    20581 gggatcgtcg gtgggctcgc cctcagccgt caacgcccac tgcccgggat agcgccggcc
    20641 ttcgcgggcc agcctctgcg tcattgacgt tgtggtgatg ctggaactga tgtcgatcat
    20701 caccggatca ccgtcggtcg gatacccttc cgccaagggg ttcggtgtga ggacggggcg
    20761 gatgccgccg aaaggcgcca tccgggctgc ggaagccgtg gagcacttga tctgcgcgat
    20821 gtagcctttt tccgccgcat cacggagata cacggccagc gcaccgatgt gatgtgaatt
    20881 gccgatcgcg aaggtgacca cgccgtgctg cttcaggcgc tcatagccaa gtgccatggc
    20941 tttcttgacc agccagcctc cggggagtct ctggccgtcc cacgtgacac agctgccgcg
    21001 atcgctcacc accttgatgt cgccgctacc gcgcatcctc cccgtcgcca gcgcatccaa
    21061 ataggaatcg agaagcgcga cgccgtgcgt attgtgcccc atgagatcgg catcgagcag
    21121 gacatcgctg acgacctccg ccttgtccgg atcgacgccg gccgcttcga gtagagcgac
    21181 gacgttccta gagagagcga tgtgattgac tgttgccatg gggttgctcc gcgggcgcct
    21241 tgccagtcgg gcgcagtaaa tcaagccggc ctcgcccgag cgagactaca gccggacagg
    21301 ggtcaaaagc ggcttgccgg cgaagaacgc ggccaggtta tcgaccacaa gctgggccat
    21361 tgcatctcgt gtctcgaccg tgcccgaggc gtggtgcgga tagagcgtga cattgcgcaa
    21421 ggagacgaga cccgcctctt gcgtgggctc ctgcgcgtag acatccaacg ccgcatgacc
    21481 aaggcgcccc tcacgcagcg cggtgaccat cgcgtcctga tccaccactg agccccgcga
    21541 gatgttgatc aacagcccct cgggaccgag cgcatcgatg acgtcgcggg aaatcaggcc
    21601 gcgcgtgccc tcgccccctg taaccgtgac ggcgaggtgg tcggactccg cggcaagcgt
    21661 gcggacgtcg ggccgatatc gccagccgac atccttggca ctccgattcc agtacgacac
    21721 ctcgacgccc atcgactctg cccgctgcgc gatcgcggtc ccgatcatgc caagcccgac
    21781 gatgcccaga cgactgcctt tgagcgcccg ctgcaacggg aacattcctt tccgcaccca
    21841 gtcgccggaa cggacatagg catcggcctc gaccaacccc cttcgcgcgg cgagtagaag
    21901 catgattgcc gtgtcggcga cgtcgtcgcg cagtgcaacc gaggtgttcg tcaaggcgat
    21961 tccgcgctcg gccatgaggc cgagatcgaa ggtctcgaag ccggcggagg agcacgccac
    22021 caagccaaga tttggcaacg cgtcgagcat cgtcctgtca atcctggcat gcccattggt
    22081 cgccaccgcc cggcacttcg caccgtgttc cgcaaggaaa gcggtcttgt cggccgcctg
    22141 gtcccagcga cgcagggtat aggcctcttc caattgagcc attgctttcg ggcgttgctc
    22201 gtacagcacc atcacatcag gtttggcggt catctcatgc cttcgatacg tcaatcggtt
    22261 tcttgcgggt ctgatcgttc attgcgcatt ctttcaagcg atatcctcgg gcaacaggct
    22321 ttccgggatg ttctggtagc agaccggccg caggaaacgg cggatcgata gtgtgccgac
    22381 cgaagtcgcc ccgaaattgg tggaggccgg ataaggacca ccatgaacca tggcgtcgca
    22441 cacctcgacg cctgtcggga aaccgttggc cagcacgcgg ccggccttgc gctcgagaat
    22501 cgcgagcaat cggcggccga gcccgctatc gcccgcgtca agatgcagcg tacaggtcaa
    22561 ctgcccttcg atgctggacg cgatcgccat catctgttca tcgtccctcg ccgtgactat
    22621 cagcccaagc ggcccgaaca cttcctcggc aagctcggca ttggccagcc agtcagcccc
    22681 tgaaaccatg aagaggtagg gtgacgcatt gcgcagatcg caagtggaag tcagcaattc
    22741 gcgcacaccg tggccgcccg cgatgcgatc gcgccctttc cgataggcct ctgcgatacc
    22801 gttcgtcagc atgacttgcg cgccgacctc cgagagggcc tgccgcgagg cctcggcaaa
    22861 agcgccggcc tgcgcttccc tgatcacggc aattccggga ttggtgcaga actgcccggc
    22921 cccgagcgtc agcgagccgg cccagccctt ggcgattgcc gcccctcgcg cggcgactgc
    22981 ctcgggcaac aggaacattg gattgaccga ccccaattcg ccaaagaatg ggatcggttc
    23041 aggacgcgca gcgcaaagat caaagagtgc gcgaccaccg ccgagtgaac cggtaaagcc
    23101 gaccgccttg atcagcggat gctgcaccaa tgcgtgtccg acatcacgtc tgccgccctg
    23161 cacgagactg aagacgcctt tcggcatgcc acatcggaca acggcggcat gaattgcctc
    23221 cgcaacgatc tcgcctgtcc cgggatgggc ggaatggcct ttgacaatga cgggacaacc
    23281 tgccgcgagc gcagccgccg tgtcgccgcc ggcggtcgag aaggcgagcg gaaagttcga
    23341 tgccccgaaa accgcgaccg ggccaatcgg ccgctgcatc atgcgcatgt cgggccgcgg
    23401 cgcaggttgc cgatcaggca gcgccttatc atgacgacga tcaagatagc cacccttgcg
    23461 gatatgctcg gcgaaaaggc gaagctgacc ggtggtgcgg ccacgctcgc cctcgagcct
    23521 gccggcgggc aggccggtct cttcgcagcc gatcttggtg atggccgccc cacgcccgtc
    23581 gatctcttcg gcgatgatgt cgaggaagcg ggcgcgctcg tcccgacttg aatagccata
    23641 ggaccggaac gcctcttccg ccgccttgca ggcctggtcc accagagcca ccgttccgac
    23701 ggaaaagtca tgtggcacgc cagtggccgg cgcggaacgg aacgtctctt ccgtcttgat
    23761 ccattcaccg gcgatgagat gcccgccgtg gggctcaaaa gaattgttta gcatttgatt
    23821 cctttcttgc agccgtcaac cgcggccaat acagggcatg gtcgtcgcca tgaccgtcat
    23881 gaattgaaca ttcgcctcga ggggcaaccc cgccatgtga aagaccgacg aggccacatg
    23941 ctcgacatcc atcatcgcct ccgaccggat cgagccatcc gcctggggca cgcctttcgc
    24001 ctgcgaggcg gccatgtcgg tgcgggcatt gccgatatcg atctggccac acgcaatccc
    24061 gaaggggcgt ccatcgagcg acagcgaacg ggtcatcccc gtcaccgcgt gtttcgaagc
    24121 agtgtaggcg atcgaacccg gccgcggcgc atgcgcggag atcgaaccgt tgttgatgat
    24181 gcgaccgccc tgcggtgtct ggcgccgcat acgcgcgaac gccgcacggg cacaatagaa
    24241 gacgccggtg acgttgacgt cgatgacggt cttccattga tcgacaccga catcgtccgg
    24301 caggaccgac ttgagcccga ccccggcatt gttgaacagg acatcgagat gcccccatcg
    24361 cgcaagcacc ctgtcgaagg cctcgtcgac ctgctgcgga tcggtcacgt cgcagggaag
    24421 caccagtgca ttttcgtttc cagacgcagt ctcggccagc gcgtccgcgc ggcggccaac
    24481 caggccgacc cgccatcctt catcaagaaa ccgccgcgcc gtcgcccgcc cgattccccc
    24541 cgatgcccct gtcacgataa tggttcgttc caacacgatt ctcctttggc aaatttcaac
    24601 gcgcgttcag acaagcccat tggccaacag gaacgccctc atccgcctct gctgggcctc
    24661 ggtcaacggc cacgctgacg gcggtcgcgt ggacccgcaa tcaaacccct gttcggccaa
    24721 tgccgccttg acacctgtga cattagttcc gttcaactct gcggcacgaa tgtcttcgaa
    24781 gactttcatt ccggcaatca acctgtttgc ctcgggataa tcgccagcct ccaatgccgc
    24841 gtggatcgcc atcgagcgtt ccggccagac attgatcagg cccgaggtga aaccacgcgc
    24901 gccgaccgca tagaaagccg gtgcccagac ttcggcgagg cccccgaccc acgtgatcga
    24961 agggtcgcag gcggctttcg ccgccgcgag gttttgcgga ttcggcgtcg cccatttgac
    25021 acccttcaca ccaggcaagt cgcacagggc cttgatgcct tcggtgccga tcgcgtcatt
    25081 gcgcaggtag agcatcatcg gcaacccccc acccgccgtg gcaatggcgc gcacgtaatc
    25141 gacgatgccg cgcggcgcga cgaacgggtc cggcggctga tgaaccatga gcgcatctgc
    25201 cccggcttcg acggacatct ccgccagcct acaggcatcg cggatgccgc gcccgacacc
    25261 ggccaggacg ggcgcgcgcc cgccgaccat cgcgaccacc tcgcggacca ttacacccgc
    25321 ctcatccgtc gtcaacgcat agaattcgcc ggtgttgccg ttgacgaccg gcatgtgcac
    25381 acccaccccc agcgccctgt caatgatcgg tgcaagcctt tctggagcca cgttcccttt
    25441 tccatcatag ggcgtaacca caatgcccga aataccggca agcgccgcgt cgagatcgta
    25501 tgccattgac tctttctcca ccaaggttcc tatattttta gtatgcgtat tcatttagta
    25561 cttattcgag ctaaatgacg aaaaatatta gcataaaagc tattgataaa aaatattttt
    25621 atctatatta tttttttaat ggatgcgcaa tcatatggct acgaaaaatt gaaaagcacg
    25681 tttcaagtac aatgcggagc tatgctggcc gtcaggctcc gtaggggtta ctcaacaggc
    25741 ccagcgaagg ctgttcaggt aaaaacgaaa gacaacgcgc ctatcaccac agccaccaga
    25801 ttgtcacaaa acgatacaaa cggcgatgaa taagtctcag aatccgcgag caaaagccgt
    25861 gggcggccgc cccggcggac gggccaccat ggcggcgatc ggccgattgg cgggcgtcag
    25921 ccaagtcacg gtttcacgcg ccttatccga cccgtcgaaa gtgtcgccgg caacgatgca
    25981 gcggatccag gacgccatcg ccatcaccgg cttcgtgccg aacgccgtcg ccggcgccct
    26041 ggcttcccgt cgatcgaaac tcatctcggc cctcgtgcct tcgctgacaa acctcgtcta
    26101 tgcatcgatg atgcaatcct tcagcgactt gatgcgcgaa aacggctatg agctcctgct
    26161 ctccgaatgc ggctttcaac cagaagccga ggaaaaactc atcgccgccc acttgtcgcg
    26221 cagaccggac gcgttctatc tcaccggcat ccaccacacc gccagggccc ggcaaatgct
    26281 gatcggggcc gatattcctg tcgttgaagt ctgggacctg accgacgcac caatagacat
    26341 ctgcgtcggt ttccatcacc gggaggttgg ctgggccatg gccgacttcg ccctggaagc
    26401 cggctaccgg aacgccggca ccgtcagcgc cggcgatgaa cgcgctctcc ggcgccgcga
    26461 ctctttcgtc gaccgatttt ccgcacgagg cggcacgtgc gtgggaaatg tcagcttcaa
    26521 cggttccgca agcgtggcca ccggccgcat tgggctgacc cgcctcatcg aagagcatgg
    26581 gttttcctct gggctgatcg cctgcagttc cgatctcatg gcacaaggca tcctgatcga
    26641 ggcgcaaaag cgcaacctga gcgttccggg ccaaatcgcc gtcctcggct tcggggacca
    26701 ggactttgcc gcgtttctcg aaccggccct gaccacggtg cgggtggatc gtcccgctct
    26761 cggcaccgct gcggcccagg cggtcctcaa gcgactgcgg aacgatagcc ccgaacactc
    26821 gctcgtggac atcggctttg aactgatacg tcgggcctcc gcctaggccg cgatgctcat
    26881 atgccgccct gggccacagc ctcgggaagc tcgaattcca gcatgtaaga actcaggcac
    26941 ttcccatgcg cgtccagcgc gagcgagcgc gtcacccctc cgcccagggt gtgggtcata
    27001 acgaagttca aggcgccaag ttgtggcagc tcataccgcg tgaccacacc cttcacgatc
    27061 cagctgaaat gtgccttgac ggcgtcgggc gtcagatgac ggcgcaacaa ctcgtagtgc
    27121 gcggcatcga aggctatgac agaaatattg gaggtgtcgc ccttgtcgcc ggtacgggag
    27181 tgcgcaatgt cgcggacttt catgatcaga cctccaggta gtcgacggcg ggccgtatgt
    27241 gttcacgggg cagcagcacg gacagcacgg caatgacttc ccgggcggac ttggtgacac
    27301 caccgccccc cgccggcccg ttggtgtaga gcgtttcgac ttcgttgccg acacgcaccg
    27361 cctcggccag ggaatcggtg cgggcggcca cccgcacacg cacttcgctc ggctcgacac
    27421 tatgcgggcc accgtggatg gcgttgaggc cgatcagatc accacgcaga tcggacaacg
    27481 cgacgctact cagccgcaac cgctcttcca cgatttccag ggccagctcc ccacgcggcc
    27541 gacaaccggg ccctgcgtag gaaatctgac cttccccgat gtacccgtcg gaataaccaa
    27601 ccgacacttt cagcgtatcg gtgcgcggtc gcccgcaggc gcccgccaca cgcacccgat
    27661 cgcgtccatc ggccgccatg gtgacttgag aaaaatcagc gatcacatcc ggcgtgtaat
    27721 aactgatcgg atcgtggatt tcatagatca tctgttcttt gcaggtggcc aacgtgacct
    27781 gcccgcccga gccgggcact ttggtgatga ccgccgagcc gtcttccgcc acctcggcca
    27841 aaggaaaacc caggcgggcc aagccttcga catccttgac acccggatcg gcgaaatagc
    27901 ctccggtcac ctgcccggcg cattccagca gatgaccgac gagcgtgcct ttgcccagca
    27961 agtcccaatc atcgagagac cagccaaact cgtgaatgag cggggcaagg aacagcacgg
    28021 gatcggcgac gcggccggtg atgatgacat cggcgccggc ccgcaacgcc tcgagcaatg
    28081 gcgccacccc gagataggcg ttggcggaga tcgccctgcc atccagcgtg cggatggact
    28141 cgccattctc gagaatggtc agcgaggacc ccttgaccac ctccaggacg tcgtcgccag
    28201 tcacgtacgc gatcttcagc cctgacaggc ccagctcttc cgccacgtcg cgggtcttct
    28261 gcgcggcggc ccgggggttg gccgcgccca tgttggtgat cagggtcacg ccattgcggc
    28321 ggcagggcgg caggacggcg cgcatgcgcg cttccagcaa aggatcgtac ccggcatccg
    28381 ggtcattctt ccgggccagc tgtgccagcg cgatggtgcg ctcggccagg cactcgaacg
    28441 ccagatagtc gagccgccct ttctcggcga gctcgactgc gggttcgatg cggtcaccgg
    28501 aatatccggc gccggatccg attctgacgg ttttcatgat gatgctccca gcctgcggta
    28561 tgcgtcgcga ccacggcacg ccagccgccc gggtcgccac gatggccgca tgcgtttccg
    28621 ctgtattcta tccaggcttg acttacaaac acttgatggc gggcgagcac ccggcgtcga
    28681 aacaccatac gaacacgaac acaggaacca tgagcgacat cacctcccga caggaactct
    28741 tcaggctcga cgacaaggtc gtgctggtca ccggctgcgg ctccataggc gaaggctggg
    28801 gcaacggcaa ggccatggcc gtgctgtgcg cccagcaggg cgccagggtg tacggcgtgg
    28861 acatcaacct cgaggccgcg caggccaccc atcatgaaat cgacaggcgc ggcgggacat
    28921 cgttcgtcga acgacgcgac gtcacagcgg ccgacgaggt caaggccatg gtcgaggcct
    28981 gcgtcgaacg cttcggacgc atcgacgtgt tgatcaacaa cgtggggcgg tccgagccgg
    29041 gggaccccgt gtccatgaac gaagacacct gggccggaca attgcagatc aacctgaaca
    29101 cagccttcct gaccatgaag caggtcatcc ccctgatggc cgcccagggc ggcgggtcca
    29161 tcatcaacat ctcgtcggtc gccggcctgc gctacgtcgg caagccgcag gtgggctacg
    29221 ccgccgccaa ggcggcactg atgcagatga cgtccaccac cgcggtgatc cacgcaaagg
    29281 aaaacgtacg catcaattgc atcgttcccg gcctgatgca cacaccgctc gttgcacgcc
    29341 tggcggaaaa atacgccggc ggcgactacg acgggttcgt gcagacgcgc aacaaccagg
    29401 tgcccatggg ccgcatgggc gatgcctggg acgtggccca tgcggcgctc ttcctcgcat
    29461 cggaccagag ccgctacatc acgggcatcg aactggtggt cgacggcggc ctgacctgcg
    29521 ccacccggta gcggtggccg cgccggcgcc gcgcctggca cgcgtttcgc gcgacacaga
    29581 tccggaaaat ccgttaccct ggcaagtttc cactcctcac tgcaagaagg aattccccat
    29641 gacgcaagcc tccgcccgcc acatcctggt cgacagcgaa gagaagtgca aacagttgaa
    29701 gaccgacatc gagaacggcg ccgatttcgc cgccgtcgcc aaggaacatt caagctgtcc
    29761 gtccagccgc gacggcggca acctgggcac
//
LOCUS       DASTNK010000051        21445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_776433, whole genome shotgun sequence.
ACCESSION   DASTNK010000051 DASTNK010000000
VERSION     DASTNK010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 21445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21445
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776433"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /gene="folD"
                     /locus_tag="VFR20_03920"
     CDS             <1..761
                     /gene="folD"
                     /locus_tag="VFR20_03920"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF010788.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEU0229694.1"
                     /translation="RIGKTPILATILVGGDPASATYVKMKGNACRRVGMDSLKVELPE
                     SITTEQLLAEIARLNANPDVHGILLQHPVPAQIDERACFDAIALEKDVDGVTCLGFGR
                     MSMGERAYGSATPAGIMSLLSAYDIPLSGRHAVVVGRSPILGKPMAMMLLAADATITV
                     CHSRTRDLPALVRQADIVVGAVGKPEFIKSDWIKDGAVVVDAGYHPSRVGDIELGGVM
                     SRAFAYTPVPGGVGPMTIATLMRHTVEAGERLIR"
     gene            complement(908..1861)
                     /locus_tag="VFR20_03925"
     CDS             complement(908..1861)
                     /locus_tag="VFR20_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0229695.1"
                     /translation="MALTRVAVVAFDQIRPFHLSVPCAVFAETPDTQAQLFDLRVCAA
                     EPGELRTQAGFSIATQHTLRTLSWADIIVVPSWRDPRETPPEPLLAALRRAHERGALV
                     VGLCLGAFVLAAAGLLDGHRATTHWRWASRFAERYPAIHVDPNVLYVDEGDILTSAGT
                     AAGIDCCLHVVRRLCGAEAAGHIARTLVVPPHRQGSQAQYIEQPLLQTTKETPLTTTL
                     DWAASHLEQTHSLDSLAARAAMSRRSFTRHFRQHTGTTVGQWLLTQRLALAQRLLEAT
                     TQPVERIAEQAGFGTPLSMRQHFAAALNTTPSMYRREFRGC"
     gene            2017..2661
                     /locus_tag="VFR20_03930"
     CDS             2017..2661
                     /locus_tag="VFR20_03930"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HEU0229696.1"
                     /translation="MTQSPAPHRALIVIDVQNEYETGGLRIQYPPMRDSLRNIGRAMD
                     AAHAAGIPVIVVQQSSPTDSPLFATGSHGWELCEVVASRPHDHYLRKQLPSAFAGTDL
                     GEWLKGHAIDTLAVVGYMTHNCNDTTIKHAFDTGMQVEFLMDASGSVPYANRAGFATA
                     EEIHRVFAVVEQSRFAAVMTTDEWVGHVTEGTKPERDNIHASHQRALARVAVAA"
     gene            complement(2756..3472)
                     /locus_tag="VFR20_03935"
     CDS             complement(2756..3472)
                     /locus_tag="VFR20_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011355972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0229697.1"
                     /translation="MDKPRVLIVEDESDIRRFARMALEQEGIDVFEASTARQARMESV
                     SRKPDLVIVDLGLPDADGKTFIQELRGWSSAPVVVLSAREQETEKVAALDAGADDYLV
                     KPFGVPELLARVRAQLRRAALTPIEGSSSTVVRFGQVCANLTTHEVTRGGQPVHLTRI
                     EFRLLGALIRGHGKVLTSRQLLQEVWGVHHTERAHYVRIYMMNLRQKLEEEPAMPQYL
                     LTELQVGYRLAGVEVVTIKE"
     gene            complement(3451..6147)
                     /locus_tag="VFR20_03940"
     CDS             complement(3451..6147)
                     /locus_tag="VFR20_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HEU0229698.1"
                     /translation="MKDLRPNPDELLKAVTEEAARTTRGRLKIFFGACAGVGKTYAML
                     QAAAQRQAEGVSVVIGIVETHGRPETGDLLDGLPALPLKRLDHREHVLQEFDLDGALS
                     AGYQLVLVDELAHSNVPGSRHAKRWRDVEELLDAGIDIYTTLNVQHLDSLNDVVGGIT
                     GIRVRETVPDRIFDAAAEVILIDLPPDDLLVRLGAGKVYLDAAAQRARQNFFRKGNLF
                     ALRELALRRVADRVNSDVRSYRISNAIQTVWPTHERLMVCIRTGRSQETLLREGARLA
                     QRLQADWIVVHVDELRRGRHPEERENLRALAESAKALGAEFTNLAGEEVAETLLDYAH
                     TRNVTKMVIGRAVTRGWRPWRAGVAERIGRANPEIGLILIGHGGPTPPAARPRQPAPS
                     PAPSGYLPRLALATLSCGLATLVASWLLRVFDLSNVVMLFLLTVVLVALRLGRAAGAW
                     SALLCVALFDFFFVEPRWSFTVADTQYLFTFTLMLTVALVISQLAARLRAEAKMATEG
                     ERRAAALARVARDLSAAIKAEQIVAICTDTIAPLLSVRVALILPDLSSKLIAVQNTGF
                     VDLTVAQWVYDHTQPAGLGTETLSGASARYIPLKAPMRVRGVLALYPADGAIPADPDD
                     RRLLDAFCSSIAMALERIHFVEIAQDTLVKMGGERLRNALLAAISHDLKTPLTAIRGL
                     AETLEQPERLPREQHVELASAIRVQAEELQRLVLNLLDLARMQSEGVRLNRDWHALDE
                     IIGSALARSTRVLAGRRVKTDLPADLPLVDVDALLMERVFVNLLDNAAKYAGTGATIL
                     LKARASDDAIHVFLEDDGPGFQVRDTEMLFEPFERGQKESSNAGVGLGLALCRRIIGA
                     HGGAIRAEQLQPHGACFEVRLPRGNPPEIEPEEAEPWTNPAY"
     gene            complement(6280..8364)
                     /gene="kdpB"
                     /locus_tag="VFR20_03945"
     CDS             complement(6280..8364)
                     /gene="kdpB"
                     /locus_tag="VFR20_03945"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEU0229699.1"
                     /translation="MSQTPTSSIEQAATFPKTAKPLRWRSILIEAFRKLAPRTQWRNP
                     VMFVVYLGSIATTLLWLQALDGSGDAPAGFILAVACWLWFTVLFANAAEALAEGRGKA
                     QAEALRATRKRVYAKVLNEPHYGGASYRAPSDELVVGTVVLVTAGDIIPADGEVIEGV
                     AAVDESAITGESAPVIRESGGDFSSVTGGTKVLSDWIVVRVTANPGEAFLDRMIAMVE
                     GAKRGKTPNEIALTIFLVALTIIFLLVCVTLLPFSSFSVLLARAGSPVSLTVLIALLV
                     CLIPTTIGALLSAIGIAGMSRMMRANVLATSGRAIEAAGDVDVLLLDKTGTITLGNRE
                     AVAFKPAPGVDERALAESALLSSLADETPEGRSIVALAKGRFGLHAQQPETSLAIPFT
                     ARTRMSGLDIGARKIRKGAASAIRADIEARGGVFPAEVEELADDISRRGGTPLAVYDG
                     CKVMGVVELKDIVKSGIKERFADLRKMGIKTVMITGDNKLTAASIAAEAGVDDYLAEA
                     TPEDKLRLIREYQADGHLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLD
                     SNPTKLMRVVEVGKQMIMTRGALTTFSVANDLAKYFAIIPAAFVATYPALGALNVMGL
                     HSPASAILSAVIFNALIIVVLIPLALKGVRYRAEPAHTLLWRNLMIYGVGGVIAPFIG
                     IKLIDMLLTPFF"
     gene            complement(8376..9050)
                     /gene="kdpC"
                     /locus_tag="VFR20_03950"
     CDS             complement(8376..9050)
                     /gene="kdpC"
                     /locus_tag="VFR20_03950"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HEU0229700.1"
                     /translation="MQPDNRDIHHASTLRGLSRPAAVSAFLFMLVTGIGYPMLTTGTA
                     RLLFPDQSQGSLIRRGGAVVGSTLIGQDFTQPWYFHPRPSATLGTDPSDPRKTVDQPY
                     NAAASGASNLGPTSGKLTDQVKSRIDAYRLENGLTANALVPVDAVTASASGLDPDISI
                     ANARLQAKRIAGARGVPTQKIMALIAQTTTPRQWAVLGEPRINVLRINLALDASFPRA
                     APGQTR"
     gene            complement(9061..10764)
                     /gene="kdpA"
                     /locus_tag="VFR20_03955"
     CDS             complement(9061..10764)
                     /gene="kdpA"
                     /locus_tag="VFR20_03955"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEU0229701.1"
                     /translation="MSNNLVQFAIVLVVMTGLAVLVGKWLARAYTSPHHTLAERWTYR
                     LLGIDPSETMPWKRYGMALLLSNAAMMFLGYLILRLQGVLPFDSLDRAAQSPDLAFNT
                     AVSFTTNTNWQAYAGESSLSNFSQMAVITFLMTISAATGIAAAGGFIRGLSRHDSADI
                     GNYWVDFTRITYRILLPLSFVLALVYVWQGIPQTLAADAWAITLEGAKQHLILGPVAS
                     LESIKHIGTNGGGFFSMNAAHPFENPTPLANTLHILGMLLIPSALTYTFGSMIARRRQ
                     GWAFFAAFLVMLIGFLAVIYSAEQHGNPLLTQLGADQQLTTTQPGGNLEGKEMRFGIA
                     QTSLFATVTTAATTGSVDAMHDSLTPLGGLVTIAQMMLNDVFGGVGVGLINLITDAIL
                     TVFLVGMMIGRTPEFLGKKIEARELKLVMIAVLAHPLSILGFTALAAVMPSTLDSLAN
                     PGPHGFSEVLYAYASGTANNGSAFAGLNADTPFFNTTIGLAMLIGRYITLFPMLAVAG
                     CLAAKKHIPESAGTLSTSTPLFTSLLIFVVLVVGGLTFLPALALGPIVEHLLMLNGQL
                     F"
     gene            complement(10900..11001)
                     /gene="kdpF"
                     /locus_tag="VFR20_03960"
     CDS             complement(10900..11001)
                     /gene="kdpF"
                     /locus_tag="VFR20_03960"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEU0229702.1"
                     /translation="MTTMGSTTLWLAGIVSALLFIYLFYALIKPERF"
     gene            complement(11295..12071)
                     /locus_tag="VFR20_03965"
     CDS             complement(11295..12071)
                     /locus_tag="VFR20_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU0229703.1"
                     /translation="MSSTQNNATAVRAFRIIEILSEAQEPLSLAQISDRIDLPKQSVH
                     RLLKQLESAWLVTRNSPGKHYECSSRVRQMAINMLMVSGPAAARRAILQQLVDTVDET
                     CNLTMAGGDGIVYLDRVEANWPLSTRLGAGTRVPLHCTSSGKLLLSFMPRQQRERLIE
                     KLPLRVSTQQTITSRDVLREELVRIRRNRLSFNNQEYMSGIVGMAVPVMLDSRRACAA
                     VAIQSSIERTSVEGLATHLPQLRDVAEEMAAVFRENHASI"
     gene            12228..13667
                     /locus_tag="VFR20_03970"
     CDS             12228..13667
                     /locus_tag="VFR20_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0229704.1"
                     /translation="MELQEIRLGTEATEADRVGQLIQKARSAQIMFSGMGQEALDDAV
                     AAVGWAIMEPGRNRLLSERAVAETGLGNVADKIRKNDRKTLGLLRDLQGVITTGVIAR
                     DEVTGITEIARPVGVVAAITPSTNPAATPANKIINALKCGNAVIVAPSPKGLGPCQML
                     IGFIHEQFKKCGLPADLVQCLPSPITKAMTAELMRQADLVVATGSQSNVRMAYGCGTP
                     AFGVGAGNVASIVDGSADVADAAAKIVASKTFDNATSCSSENSVVVVSSVYDAMLAAL
                     REQGAVLLTAAEKQRLQAVMWSSGKLSGAVTGKSAAEIADIAGLDLCDRIASDPLTVL
                     LVEEAGFGRDHPFSGEKLSPVLTVYRAPDFDGACAMVWRQYAYMGAGHSVGLHTRDEG
                     QAEVLGQALPVSRVIVNQAHCFATGGNFDNGLPFSLSMGCGTWGGNNFSDNMHYRHYM
                     NITRVARRIPERKPTMGQVLGGYFERYGA"
     gene            13664..15187
                     /locus_tag="VFR20_03975"
     CDS             13664..15187
                     /locus_tag="VFR20_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0229705.1"
                     /translation="MNEPLTFAGLLRGLASRQPDSSFLISSDSGRELTCGRLWQHCQG
                     LGRQFKTLGLGTGDKVSVYMPNGELTAVLLLGIMTNGLVANPINLMCQPAQLRHILEH
                     SDTRLIFAGREQAGALKAALREVKRDVRLVVCDPAGDEIPSFALDPAAGGTMTEPQPG
                     DPALLMYTSGTTGVPKGVLHSHDSLIYNGRCISEAHHLARGDRVLGSLPLYHINGLVV
                     TLVAPLVHEGSVVMTPRFSVASFWTDASRFRCTWINVVPTIISYLLNEGSDAATPDLS
                     RLRFCRSASSALAPEHHRAFEKRFGIGIIETMGLTETAAPAFSNPYEQDDRRIGSIGK
                     PSGTRAMVVGADGKPVASWEKGEIILTGHNVMLGYYKDPERTRDVFTDDGWLRTGDIG
                     HQDEQGYFYVTGRAKELIIKGGENIAPREIDEVVIKHPSVLDAAAVGVPHPDYGQDIA
                     VYLVLRDNAVFDSDALRRHCLSELGKYKTPSKYVVVDELPRGPSGKVQRLKLLELDA"
     gene            15360..16682
                     /locus_tag="VFR20_03980"
     CDS             15360..16682
                     /locus_tag="VFR20_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0229706.1"
                     /translation="MQRRVKTHHLILMVMCLLYFISYIDRVNISVAAPLIRDEMGLSN
                     LELGLVFSAFAYPYAAMQIFGGWLADRFGPRKVLITLSLIWGAATVLTGFVGSVIALL
                     VMRFALGIGEGGAFPTATRAFTYWMPVTQRGVAQGITHSFSRLGGAITPPLVVLIIAL
                     AGWRESFIVLGAISLVWTVLFIFVFRDNPAEHPRVTPAELAEINAGRVEQPKKEKGPT
                     PWGRLIKGMWKVTFVDFCYGWSLWVYLTWLPSYLKDARGFDLKAMAIFTALPLLAGVV
                     GDTLGGVVSDKLLRKTGNIRFARVSVLTFGLVGALLCMVPMVSMKDPLMAVLLLSLSF
                     FCLELTNAVLWSLPIDIAGDYAGTAAGMMNTGFGVAGMISPVVFGFLIQSTGSYHLPF
                     LVSAGLLGVGALTALLIDPGQTVKREPDVQGEGRRFEKMGGDTSLGVE"
     gene            complement(16819..16895)
                     /locus_tag="VFR20_03985"
     tRNA            complement(16819..16895)
                     /locus_tag="VFR20_03985"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16859..16861),aa:Met,seq:cat)
     gene            complement(17269..18183)
                     /locus_tag="VFR20_03990"
     CDS             complement(17269..18183)
                     /locus_tag="VFR20_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0229707.1"
                     /translation="MDLRQLRYFSCVAEELHFGRAARKLCISQPALSADIRRLEQELD
                     VQLLERSSKAVTLTNAGQVLLREAKRLLQQAEEARHLTLMSARGIMGRLRIGFVNSLI
                     HRGLPEALKRFEADHPTIDVTLSEMNTTEQIDALLRNQLDMGLSHSARFPASIRAERL
                     AVEPFLCCLPLRHPLAERASLSVKDLRDDAFILFPRRVSPYYHDQIIALCVEAGFSPR
                     IKHEVRLWQTVVGMVEKGMGIALVPRSIALACGRRVACARLDGVSAVSETLCLWNGDT
                     MPAAAQLFAGYIRQALPETGRDSATSSE"
     gene            18367..20151
                     /locus_tag="VFR20_03995"
     CDS             18367..20151
                     /locus_tag="VFR20_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0229708.1"
                     /translation="MSQAASAKPSAIPDSRGTNLFDRDPAFDALLRLYLPEALHAHLL
                     PILRRLGRQAGGELDRLAASADRNPPVLAPRTRGGADIDSIEKHPDYVALERVAYSKL
                     GLAAMSHRDGVLGWSEPLPAVAKYALTFLFVQAEFGLCCPVSMTDSLTRTLRKFGAPD
                     LVARFLPQLTSQDFDQLYQGAMFMTEQDAGSDVGGVATRAHQDGGQWRLTGDKWFCSN
                     TDAELAMVLARPDGAPKGIKGLGLFLLPRTLPDGSRNHYRIIRLKDKLGTRSMASGEI
                     RLDGAIAYPVGDLTRGFKQMADMINMSRLSNGVRSAGLMRRAYSEAHYVASHREAFGK
                     RLMDMPLMRRQLIKMLLPGEQARSMFLHTAQCLAQSDAGDARAGKLLRIMTPLIKFRA
                     CRDARKVTGDALEVRGGCGYIEEWVDARLVRDSHLGSVWEGTSNIVALDVMRAATRED
                     ALTPLRVYLEELLDAPGIPAESRVLLQALLSRAAGLADAVARDAREHLARQAASALYH
                     VTSAIIMAWEAARQAPDWRRLALAHLVARFRLSPVDPLDDGDADALDPLMRALLADEP
                     ISAALARDCLTTSSAHAETRADEPSLSL"
     gene            20168..21316
                     /locus_tag="VFR20_04000"
     CDS             20168..21316
                     /locus_tag="VFR20_04000"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU0229709.1"
                     /translation="MNSHGALQGIKVIDLTRVLGGPYCTQLLADHGADVIKIEPPKGD
                     ETRAWGPPFDDDGTAAYFRGVNRNKRGIVLDLTTESDRAVLLRLLEGADVLVENFKSG
                     TLERWGLGYAEVLAERFPRLIHCTISGFGTDGPLGGLPGYDAVIQAMAGLMSVNGEDG
                     GGPLRVGLPVVDMVTGLNAANGIMLALQERHVSGRGQSVEVALYDAGISLLHPHMPNY
                     LASGKTPSRTGNAHPNITPYDTYRTGTVPLFLAVGNDSQFRALCRYLEAPELADDPRF
                     DSNAKRCGHRPELKRELEARFAGHDGVALASDLIRHGVPCGPLHDVAQVADHPHTRHR
                     GMIVAIDDYRGVASPIKLSRSPATYRRKPPRFGADTQAVLAELDGDRS"
BASE COUNT         3940 a         6435 c         6985 g         4085 t
ORIGIN      
        1 accggatcgg caagaccccc attctggcca caatcctggt gggtggtgat ccagcctcgg
       61 ccacgtacgt caagatgaag ggtaacgcct gccgccgtgt gggcatggat tccctcaagg
      121 tcgaactccc ggaaagcatc accacggaac agcttctggc ggaaatcgcc cgcctcaatg
      181 ccaaccccga tgtgcatggc atcctgttgc agcaccccgt gcccgcgcag atcgatgaac
      241 gtgcctgctt cgacgccatc gccctggaaa aagacgtgga cggggtcacc tgcctgggtt
      301 tcgggcgcat gagcatgggc gaacgcgcct atgggtcggc cacaccggcc ggcatcatgt
      361 cgctgctgtc ggcctatgac atcccgctgt cgggccggca cgcggtggtg gtgggccgca
      421 gccccatcct gggcaagccc atggccatga tgctgctggc cgccgacgcc accatcacgg
      481 tctgccattc ccgcacccgg gatctgcccg cattggtgcg gcaggccgat atcgtagtcg
      541 gcgcggtggg caaacccgag ttcatcaaga gtgactggat caaggacggc gccgtggtgg
      601 tcgacgcggg gtatcaccca tcgcgggtgg gtgatattga actgggcggc gtgatgtcgc
      661 gcgcgttcgc ctacacgcct gtgcccggcg gggtggggcc catgaccatc gccacactga
      721 tgcggcatac cgtcgaggcg ggcgaacgat tgatacgctg atggcgttgg cgtggttgtg
      781 ccgggtggtg ccccgattgg ctaaagattc ccttatccat ggatgtcttc cggggcgaca
      841 agctggtttg gtcggagagc tcccttcggg atggtataga gatcggggcg tgcgctgtgg
      901 aagacgctta gcacccgcgg aattctctcc gatacatcga tggcgtggtg ttgagcgccg
      961 ccgcgaagtg ctgtcgcatc gataacggcg tgccgaaccc cgcctgttcc gcaatgcgct
     1021 caacaggctg ggtggtggct tccagcaggc gttgcgccag cgccagccgc tgcgtcaata
     1081 gccattgacc caccgtggtg cccgtgtgct ggcgaaagtg gcgggtgaag ctgcgccggc
     1141 tcatggccgc gcgcgccgcc agggaatcca gactatgggt ttgctccagg tggctggcgg
     1201 cccagtccag tgtcgtggtg agcggtgttt ccttggttgt ttgcagcaag ggttgctcga
     1261 tgtattgagc ctggctgccc tgccgatggg gggggaccac cagggtgcgg gcgatatggc
     1321 cggccgcttc cgcgccgcac agccgccgca ccacgtgcag gcaacaatcg atgccggccg
     1381 cggtgcccgc ggaagtgagg atgtcgcctt catcgacata cagcacgttg ggatcgacat
     1441 gtatcgccgg gtagcgctcg gcgaagcgtg aggcccaacg ccagtgtgtg gtggcgcgat
     1501 ggccatccaa caaaccggcg gcggccagca cgaaggcccc aagacacagg ccgacgacca
     1561 gggctccacg ctcgtgggcg cgccgcaagg cggccagcag tggctctgga ggcgtttcgc
     1621 gcgggtcacg ccaggacggc accacgatga tgtcggccca ggatagtgtc cgtagagtgt
     1681 gttgggtggc gatgctgaaa cccgcctggg tgcgcagttc accgggttcc gccgcgcata
     1741 cgcgcaagtc gaacaattgt gcctgcgtgt ccggggtctc ggcgaacacc gcacagggca
     1801 ccgacaaatg aaacggccgg atctggtcga acgccacgac cgcgacgcgt gtaagagcca
     1861 tgtccctctc cgtagtcttg aatggcccga ttataactaa aaatggcatt caggccactt
     1921 ctggaatatc caccggcaac ggataatgat ttcaccggga ctgcttgggg atcctctgaa
     1981 ccccatgtcc cttcaccagc cagaaggaat accgccatga ctcaatctcc cgccccgcac
     2041 cgtgccctga tcgtgatcga tgtgcagaat gaatacgaaa ccggaggatt gcggatccag
     2101 tatccaccaa tgcgcgattc gctgcgcaat atcggccgcg ccatggacgc tgcgcacgcg
     2161 gcaggcattc cggtgatcgt ggtgcagcag tcctcgccga ctgactctcc cttgttcgcc
     2221 accggttcgc atggctggga actttgtgaa gtcgtcgcca gccggccgca cgatcactac
     2281 ctgcgcaagc agcttcccag cgctttcgcg ggcacggatc ttggggagtg gctgaagggc
     2341 catgccatcg atacgctggc ggttgtcggc tacatgacgc acaactgcaa cgacaccacc
     2401 atcaagcatg ccttcgacac cggcatgcag gtggagttcc tgatggatgc ctcgggaagc
     2461 gtgccgtacg ccaatcgcgc gggcttcgcc acggcggagg agatccaccg cgtgttcgcg
     2521 gtggtggagc agtcgcgctt cgcggcggtg atgacgaccg atgagtgggt tggccatgtc
     2581 accgaaggga ccaagcccga gcgcgacaac atccacgctt cgcaccaacg ggcgctcgcg
     2641 cgggtcgccg tggcggcgtg atgactcgag gcgcggtgga ctgcggttga tttgtttgcg
     2701 ccgcgccttg atttcatggt cactatgcag agcccccgat ttcattccgc ccggatcact
     2761 ctttgatcgt gaccacctca accccggcaa gccggtatcc cacctgtaat tccgtcagca
     2821 ggtactgggg catcgcgggc tcttcctcga gtttctggcg cagattcatc atgtagatgc
     2881 gcacgtagtg agcccgctcg gtgtgatgga caccccagac ttcctgaaga agctggcgcg
     2941 aagtcagcac cttgccgtgg ccacgaatca aggccccgag cagccggaat tctatccggg
     3001 tcagatgcac gggctggccg ccgcgggtga cttcgtgggt ggtcaggttc gcgcacacct
     3061 gcccgaaccg cacgacggtg ctcgatgatc cctcgattgg cgtcagcgcg gcgcgtcgca
     3121 actgggctcg cacgcgggcc agtagttcag gcacgccaaa cggtttgacc aggtagtcgt
     3181 cggcgccggc atccagcgcc gccacttttt ctgtctcctg ttcccgggcg gaaagcacca
     3241 cgacaggagc ggacgaccac ccccgtaatt cctggatgaa cgtctttcca tcggcatccg
     3301 ggagacccag atccacgata accagatctg gtttacggct gacggattcc atgcgtgcct
     3361 gtctggcggt ggaggcttcg aacacatcga tgccttcctg ctcgagcgcc attctcgcga
     3421 aacgccgaat gtcggattcg tcttccacga tcagtacgcg gggtttgtcc atggctctgc
     3481 ctcttcgggt tcgatttcag ggggattgcc gcgcggcagg cgcacttcga aacaggcgcc
     3541 atggggctgg agctgttccg cccgtatggc accgccgtgg gcgcctatga tgcggcggca
     3601 taaggccagg ccaagaccca cccccgcgtt ggaggattcc ttctgaccac gctcaaaagg
     3661 ttcgaagagc atttcggtat cgcgcacttg gaagccgggg ccgtcatcct ccagaaacac
     3721 atggatcgcg tcgtcggacg ccctggcttt cagaaggatc gtggcgccgg ttcccgcgta
     3781 cttggccgca ttgtccagaa ggttgacgaa cacacgctcc atgagcaggg cgtcgacatc
     3841 gaccagggga aggtcggcgg gcagatcggt cttgacgcgt cggccagcca ggacgcgcgt
     3901 cgaccgagcc agggcgctgc cgatgatctc gtcgagcgca tgccagtccc ggttcaggcg
     3961 gacgccttca ctttgcatgc gagccagatc cagcagattg aggacaaggc gctgcaactc
     4021 ctccgcctgg acacggatgg cgctggcgag ctccacgtgc tgttcgcggg gcagccgttc
     4081 cggttgctcc aaggtttcag ccagtccgcg gatcgccgtc agtggcgttt tgagatcgtg
     4141 ggatatggcg gcgagcaggg cgttgcgcaa ccgctcaccc cccatcttca ccagtgtgtc
     4201 ctgggcgatc tcgacgaagt gtatgcgctc cagcgccatg gcgatggacg agcagaatgc
     4261 gtcgagcagg cgccggtcat cgggatccgc gggaatggcg ccatcggcgg ggtatagcgc
     4321 cagaacgccg cgcacccgca tgggggcctt cagtggaatg tatcgcgcgc tggcgccgct
     4381 gagggtttct gtgcccaggc cggccggttg agtgtggtcg tagacccatt gcgcgacagt
     4441 gaggtcgacg aagccggtgt tctggacagc gatcaatttg ctggacaaat ccggcaggat
     4501 gagtgccaca cgcaccgaca gcagcggcgc gatggtgtcg gtacagatgg cgacgatttg
     4561 ttcggccttg atcgcggccg acaagtcgcg ggccacgcgc gccaacgccg cggcgcgtcg
     4621 ttccccttcg gtggccatct tcgcttcggc ccgcagacgc gcggccaact ggctgatgac
     4681 gagcgcgaca gtgagcatca gcgtgaacgt gaacaggtat tgcgtatccg ccacggtgaa
     4741 cgaccaccgc ggttcgacaa agaaaaaatc gaacaatgcc acgcagagga gcgccgacca
     4801 ggcccccgcc gcccgcccca ggcgcaacgc gacgagcacg accgtcagca ggaacagcat
     4861 caccacattg gacaagtcga agacacgcag gagccagctg gcgaccagcg tggcgagacc
     4921 acaggacagc gtggccaagg ccaggcgcgg caggtaaccg gatggtgcgg gcgagggggc
     4981 gggctgcctt gggcgcgcgg ccggaggcgt gggacccccg tgtccgatca ggatcaggcc
     5041 gatttcagga ttggcgcgac cgattcgttc agcgaccccg gcccgccagg gccgccagcc
     5101 acgtgtgacg gcacgcccga tcaccatttt tgtgacattg cgcgtgtggg cgtagtcgag
     5161 cagggtttcg gcgacttcct cgcccgcaag gttggtgaat tcggccccga gggctttcgc
     5221 ggattccgcc agtgcacgca gattctcgcg ttcctcgggg tggcgtccac gacgcagttc
     5281 gtcgacatga acaacgatcc agtcggcttg caggcgttgg gccagtcggg caccttctct
     5341 cagaagggtt tcctgggagc gaccggtgcg tatgcagacc atgagccgct cgtgggtggg
     5401 ccatacggtc tggatcgcat tcgatatccg gtagctgcgc acgtcggagt tgactcgatc
     5461 cgccacccgg cgcagcgcca gctcccgcag tgcgaagaga ttgcccttgc ggaagaagtt
     5521 ctggcgcgcg cgctgtgccg ccgcgtcgag gtagaccttc ccggctccca gccggaccag
     5581 caagtcgtcg ggcggcaagt ctatgagaat gacttcggcg gccgcatcga agatccggtc
     5641 cggtacggtt tcccgtacgc gtatgccggt gatgccgcct acgacgtcgt tcagactgtc
     5701 aagatgctgg acgttcagcg tggtgtagat gtcgatgccc gcgtccagca gttcctcgac
     5761 atcccgccag cgtttggcgt ggcggctgcc aggcacattg gaatgcgcca gctcgtcgac
     5821 cagaaccagc tgatacccgg cggacagcgc gccatccagg tcgaattcct gaagaacgtg
     5881 ttcccggtgg tccagccgtt tgaggggcaa ggcgggcagc ccgtcgagca gatcccctgt
     5941 ttcggggcgt ccgtgggttt cgacgatgcc gatcacgacg gaaactcctt cggcctggcg
     6001 ctgtgcggcg gcctggagca tggcgtatgt cttgcccaca cccgcgcagg cgccaaagaa
     6061 tatcttgagc cggccgcgtg tggttctggc ggcctcttcg gtgacggcct tgagaagctc
     6121 gtcgggattg gggcgtaggt ctttcaatgc cgattcgtgg ctggatgaga gggcccgggc
     6181 acactatggg aggcgtcgca tggcgtgggg aagggctgga gtccgccgcc gcgggctccg
     6241 ttcagatgtt atcttaccgg agcctggcgc gatggcgttt cagaagaacg gtgtcagcag
     6301 catatcgatg agcttgatgc ctatgaatgg cgcgatcacg cctcccacgc catagatcat
     6361 caagttgcgc cacagcagtg tgtgggcggg ttccgcccga taccgcacac ctttcagcgc
     6421 cagagggatc agcaccacga tgatcagagc gttgaagatg accgcggaca gaatcgccga
     6481 cgctgggcta tgcagaccca tgacgttgag cgcgcccagt gccggatacg tggcgacgaa
     6541 ggcggcggga atgatggcga agtacttggc cagatcgttg gccacactga aagtggtcaa
     6601 cgcaccccgc gtcatgatca tctgcttgcc gacctcgacc acccgcatga gcttggttgg
     6661 attgctgtcc aggtccacca tgttgccggc ctccttggcc gcctgtgtgc cgctgttcat
     6721 cgccacggcg acgtcggcct gggcgagcgc tggcgcatcg ttggtgccat ctcccgtcat
     6781 ggccacaaga tggccgtcgg cctgatattc gcggatcagg cgcagcttgt cttccggcgt
     6841 ggcctccgcc agatagtcat ccacgccggc ctcggccgcg atgctggcag cggtcagctt
     6901 gttgtcgccg gtgatcatca ccgtcttgat acccatcttg cgcaaatcgg cgaatcgctc
     6961 ctttatgccg ctcttgacga tgtctttcag ctccacgact cccatgactt tgcaaccgtc
     7021 gtagaccgcc agtggtgtgc cgccacgacg ggaaatgtca tcggccagtt cctccacctc
     7081 ggccgggaat acaccgcccc gcgcttcgat gtcggcgcgg atcgcggaag cggcgccctt
     7141 gcggatcttt ctggcaccga tgtccaggcc gctcatacgg gtccgcgccg tgaacggtat
     7201 ggcaagactt gtttccggtt gctgggcatg aaggccgaag cggcccttgg ccagagcgac
     7261 gatggaacgg ccttccgggg tttcatccgc cagcgaggaa agcagggcac tctcggcgag
     7321 cgcccgttcg tcgacgcctg gcgcgggttt gaacgccacg gcttcccggt tccccagcgt
     7381 gatggtgccg gtcttgtcca gcaacagcac gtccacatct ccggccgcct cgatggcgcg
     7441 gccggatgtg gccagcacgt ttgcacgcat catgcggctc atgccggcta tgccaatggc
     7501 ggagagcagc gcgcctatgg tggtcgggat gaggcacacc agcagggcga tgagcaccgt
     7561 caggctcact ggggaaccgg ctcgggccaa caagacgctg aaactcgaga acggaagcag
     7621 ggtgacgcat accagcagga agatgatggt cagcgcgacc agaaagatgg tcaaggctat
     7681 ttcgtttggt gtcttgccac gcttggcgcc ctccaccatg gcgatcatcc gatcaagaaa
     7741 cgcttcgcca ggattggccg tcacgcgcac gacaatccag tccgacagga cttttgtgcc
     7801 gcctgtcaca gaggaaaagt caccgccgga ttccctgatg accggagcgg actccccggt
     7861 gatggctgac tcgtcgacag cggccacgcc ttctatgact tcgccgtcgg cggggatgat
     7921 gtcgccagcg gtaaccagca cgacggtgcc tacgaccaat tcatcgcttg gcgcccggta
     7981 agatgcgccg ccgtagtgcg gttcgttgag cactttcgcg tagacgcgct tgcgcgtcgc
     8041 gcgcagagct tcggcttgcg ccttgccgcg tccttcggcc agcgcttccg cggcgttggc
     8101 gaacaaaacg gtgaaccaca gccagcaggc cacggcgagg atgaatccgg ccggggcgtc
     8161 ccccgatccg tcaagggcct gaagccaaag caacgtggtg gcgatgctgc ccagatacac
     8221 cacgaacatg accggattcc tccactgcgt ccgtggggcg agtttcctga aagcctcgat
     8281 caggatggac ctccagcgca atggtttggc ggttttgggg aacgtggcgg cttgttcaat
     8341 tgaggaagtg ggtgtttgag acataggggc tccgattacc gcgtctgtcc gggcgcggct
     8401 ctggggaatg aggcgtctag cgccagattg atccgcagca cgttgatgcg cggctcgccc
     8461 aacacggccc attggcgtgg cgtggtggtt tgggcgatca gtgccatgat cttctgtgtt
     8521 ggaacgcccc gtgcgccggc gatccgtttg gcctgcaagc gcgcattggc aatggagatg
     8581 tcggggtcca accccgaggc ggacgctgtc accgcgtcaa ctggaacaag cgcgttggcc
     8641 gtgagaccgt tctccaaccg gtaagcgtcg atcctggatt tgacctggtc tgtcagcttc
     8701 ccgctggttg gcccgaggtt gctggcgccg ctcgcggcgg cgttgtaagg ctggtcgaca
     8761 gtctttcgcg gatcggaagg gtcggtgccg agggtcgcgc tgggacgggg gtgaaagtac
     8821 cagggctggg tgaaatcctg gccaatcaga gttgaaccga ccactgcgcc gcctcgtcga
     8881 atcaagctgc cttgagactg gtcgggaaag agtaggcggg cggtgccggt ggtcagcatc
     8941 ggatagccga taccggtcac cagcatgaag agaaaggcgg acaccgccgc gggccgcgag
     9001 aggccgcgca aggtggaagc gtgatggata tcgcgattgt cgggctgcat gatgcctacc
     9061 tcagaacaat tgaccgttca gcatcagcag atgctcgacg atggggccta gtgcaagcgc
     9121 aggcaggaat gtcaggccgc ccaccaccag caccacaaag atcaacaggc tggtgaatag
     9181 gggagtggac gtggacaaag tgcccgcgct ttcgggaatg tgtttcttgg ccgccaggca
     9241 tccggccacc gccaacatgg gaaatagcgt gatgtagcgg ccgatcagca tggccaggcc
     9301 gatggtggtg ttgaagaacg gtgtgtcggc gttcaggccg gcaaacgccg aaccgttgtt
     9361 ggcagtgccg gacgcatagg cgtaaagcac ttcgctgaag ccgtgtggac ccgggttggc
     9421 caggctgtcc agggtcgaag gcatgactgc cgccagcgcg gtgaatccca ggatgctgag
     9481 gggatgggcc agaaccgcga tcattaccaa tttcaactcg cgcgcctcga tcttcttgcc
     9541 caggaattcc ggtgtgcgtc cgatcatcat gccgacgaga aagacagtca gaatggcgtc
     9601 ggtgatgaga ttgatcagtc cgacgccgac gccgccgaac acgtcgttca gcatcatctg
     9661 cgcgatggtg acgagcccac ccaggggggt gagggagtca tgcatggcgt cgaccgaccc
     9721 ggtcgtggcc gccgtcgtca ccgtggcgaa caggctggtc tgggcgatac cgaaacgcat
     9781 ctccttgcct tcgagattgc cccccggctg ggtggtggtc agttgttgat ccgccccgag
     9841 ttgggtaagc agtgggttgc catgctgttc cgctgaatag atgacggcca gaaagccaat
     9901 cagcatgacc agaaacgcgg cgaagaacgc ccagccctgt cgcctgcgcg caatcatgct
     9961 gccgaacgtg taggtcaggg cggagggaat cagcaacatg cccagtatgt gcagcgtgtt
    10021 ggccaacggc gtggggttct cgaaggggtg ggcggcattc atgctgaaaa agcccccgcc
    10081 gttggtgccg atatgcttga tcgattccag gctggccacc ggccccagga tcaaatgctg
    10141 tttcgcccct tcgagggtga tggcccatgc gtcggcggca agcgtctggg gaatgccttg
    10201 ccatacgtag accagtgcca agacaaaact caacggtaga aggatccggt aggtgatccg
    10261 ggtgaaatcc acccagtaat tgccaatgtc cgcgctgtcg tgccggctta atccccggat
    10321 gaaaccccct gcggcggcga tgccggtggc cgcgctgatg gtcatgagaa atgtgatcac
    10381 cgccatctgc gaaaagttcg acagactgct ttcaccggcg taagcctgcc agttggtgtt
    10441 cgtggtgaag gaaaccgccg tgttgaatgc caagtccgga ctctgggcgg cccgatccag
    10501 ggagtcgaac ggcaacacgc cttgtagcct caggatcagg tagccgagga acatcatcgc
    10561 cgcgttgctc aacaacaagg ccatgccata gcgtttccac ggcatggtct cggagggatc
    10621 gatgcccagc aggcggtagg tccagcgttc ggccagtgtg tggtgcgggc tggtgtaggc
    10681 gcgggccagc catttgccga caagaacggc gagaccggtc atgaccacca gcacgattgc
    10741 gaattgaaca agattgttgg acatgtcgcc gccctcagat tttgtccagg ccagccagga
    10801 atccggcggc caggatgaag aaagccagcg ccaggaatac gaaagcgatg tcgcccatgg
    10861 aaatcatccg tgttgattgt tgcgttgtgc gcgcgtgcgt cagaatcgct cgggtttgat
    10921 cagcgcgtag aacagataga tgaaaagcag ggccgagacg attccggcca gccacaaggt
    10981 tgtcgagccc atggtcgtca tgcggcgcgc aggcggttgg cgagcgcgga ggctgccgtg
    11041 gcttgggatg tggccaccgt gtcttgtgcc ttggctggat gcggggctgt tgtaggactg
    11101 atagtcactg ccatctccat gttatttgga acattcctgg aggcagtgta gagacggcga
    11161 cgtaaaggtg ccgctcattt ccgaggcggc gacataaaaa tcgtgtaaag attcttgtgg
    11221 ctatcgttct atgccggcgc acatcggtgc gcagtgctct gggtgttctg tctccgcacc
    11281 tccgagacgg cacactagat cgatgcgtga ttttcccgga agacagccgc catttcctcg
    11341 gcaacgtcgc gcagttgcgg aagatgggtc gccaggcctt cgacacttgt gcgttctatc
    11401 gaagactgaa tggcgaccgc cgcgcatgcg cggcggctgt ccagcatgac gggcaccgcc
    11461 atccccacga tgcctgacat gtactcctga ttattgaagc tgagccggtt ccgccggatg
    11521 cgcacgagct cttcgcgcag cacatcacgg ctggtgatgg tctgctgcgt gctcacgcgc
    11581 aatggcagct tctcgatcag ccgttcgcgc tgctgtctgg gcatgaaact gagcagcagt
    11641 ttgccactgg acgtgcaatg caagggaacc cgagtgcccg cccccagcct cgtggacagc
    11701 ggccagttgg cttccaccct gtcaaggtac acaatgccgt ccccgccggc catggtcaga
    11761 ttgcaggttt catccaccgt gtccacgagt tgctgcagta tggccctgcg tgctgccgcc
    11821 gggcctgaca ccatcaacat gttgatggcc atctgacgca cgcgcgacga acactcgtaa
    11881 tgcttgcccg gactattgcg ggtcaccagc catgcgctct cgagctgctt gagcagcctg
    11941 tggactgatt gcttaggcag atcgatgcga tcggagatct gggccaggga caggggttcc
    12001 tgggcctcgg acaggatttc tatgatgcgg aaagcacgta cagccgtggc attgttctgg
    12061 gtcgatgaca tgcggattcc ctcgagatag agacgactgt agtctcgaaa tgtgtatttt
    12121 tccatccacg agaacgttcc cgtcgcggaa tcatgacata tcgggacgct ttatttctcc
    12181 gttcacgtag attggacatt agtccgtcca atcaggaggt gcaggagatg gaacttcagg
    12241 aaatccgtct gggtacggaa gccaccgagg ccgaccgggt cggtcaattg atacagaagg
    12301 cgcgcagcgc ccagatcatg ttctctggaa tggggcagga ggccctggat gacgcagtcg
    12361 ccgccgtcgg ctgggccatc atggagcccg ggcgcaaccg cttgctgtcc gaacgggcgg
    12421 tcgccgaaac cgggctgggc aacgtggccg ataagatccg caaaaacgat cgcaagaccc
    12481 tgggcctgct gcgcgacctg caaggggtca tcaccaccgg cgtcatcgcg cgcgacgagg
    12541 tgaccggcat taccgagatc gcccgtcccg ttggcgtggt ggcggcgatc accccgtcca
    12601 ccaatccggc agccacgccg gccaacaaga tcatcaacgc gctcaaatgt ggcaatgcgg
    12661 tcatcgtggc cccatccccc aaggggctgg ggccgtgtca gatgctgatt ggcttcatcc
    12721 atgagcaatt caaaaagtgc gggctgcccg cggatctggt gcagtgcctg ccgtccccga
    12781 tcaccaaggc catgactgcc gagctcatgc gccaggcgga tctcgtggtc gccaccgggt
    12841 cgcagtccaa cgtgcgcatg gcctatggct gtggcactcc ggcgttcggg gtgggggcgg
    12901 gaaatgtggc gtcgatcgtg gacggctccg ccgacgtggc tgatgccgcc gccaagatcg
    12961 tggcctccaa gacctttgac aatgccacca gctgctcgtc ggaaaatagc gtagtggtgg
    13021 tttcttcggt atacgacgcc atgctggccg ccctgcggga acagggggcg gtgctgctca
    13081 ccgccgccga aaaacagcgt ctgcaggccg tgatgtggtc gtccgggaaa ttgagcggtg
    13141 cggtgacggg caaatcggcg gcggaaatcg ccgacatcgc gggcctggac ctgtgcgacc
    13201 ggatcgcctc ggatccgttg actgtgctgc tggtcgaaga ggccggcttt ggtcgcgacc
    13261 atccgttttc cggagaaaag ctctcgccgg tcttgaccgt gtatcgtgcg ccggatttcg
    13321 acggggcgtg cgccatggta tggcgccagt atgcatacat gggggctggc cattcagtcg
    13381 gcctgcatac tcgggatgag ggccaggcag aggtgcttgg tcaggccctg ccggtgtccc
    13441 gggtcattgt caatcaggcg cattgcttcg ccacgggcgg gaatttcgac aatgggctgc
    13501 cgttctcgct gtccatgggg tgcggcacct ggggcgggaa caacttctcc gacaacatgc
    13561 attatcgcca ctatatgaac atcacacgcg tggcgcgtcg gattccggag cgcaagccca
    13621 ccatggggca ggtgctgggt ggatacttcg agaggtacgg ggcatgaacg agccactcac
    13681 tttcgcgggc ctattgcggg gcctggcatc ccgccagcct gatagctctt ttctcatttc
    13741 ctccgacagc ggccgcgagc tgacttgcgg acgcttgtgg cagcactgcc aaggcctggg
    13801 gcggcagttc aagacattgg gcctgggcac aggcgacaag gtttccgtct acatgccaaa
    13861 cggcgaactg acggcggtat tgttgctggg catcatgacc aacgggcttg tggccaatcc
    13921 gatcaacttg atgtgccagc ccgcgcagct caggcatatt cttgagcact cagatacgag
    13981 gctgatcttt gccggcagag agcaggcggg cgcgctgaag gcggccttgc gcgaagtgaa
    14041 gcgcgacgtc cgactggtgg tatgcgaccc ggccggcgac gaaataccgt cgtttgcgtt
    14101 ggaccccgca gccggcggca ccatgactga gccgcagccg ggcgacccag ccttgttgat
    14161 gtacacctcg ggtacgacag gcgtgcccaa gggcgtgctg cacagccacg acagtctgat
    14221 ctacaacggc cgatgcatca gcgaagccca tcatcttgcc cggggcgacc gggtgctggg
    14281 gtcgctgccc ctgtaccata tcaatggcct cgtggtgacg cttgtggcgc cgctggtgca
    14341 tgagggctcg gtggtaatga cgccgcggtt ctcggtagcg tctttctgga cggatgccag
    14401 ccgtttccgg tgcacctgga tcaatgtggt gcccaccatc atttcctatc tgctcaacga
    14461 aggcagtgac gccgccacgc cggacctgtc ccgcctgcgg ttctgccggt cggcctcatc
    14521 agctttggcg ccggagcacc atcgcgcgtt cgagaagcgg ttcggcatcg gcatcattga
    14581 gacgatgggc ctgacggaaa cggccgcgcc ggcattcagc aatccctatg aacaggacga
    14641 ccggcgcata ggcagcatcg gcaaaccttc cggcacgcgc gccatggttg tgggcgccga
    14701 tggcaaaccg gtggcgtcgt gggagaaggg tgagatcatc ctgacaggac ataacgtcat
    14761 gctgggatac tacaaggacc cggaacgcac gcgtgacgtg ttcaccgacg atggctggct
    14821 caggacgggt gacatcggac atcaggatga gcagggctac ttctacgtca ccggccgggc
    14881 caaggaactg atcatcaagg ggggcgagaa cattgcgccg cgcgagatcg acgaggtggt
    14941 aatcaagcat cccagtgttc ttgatgcggc cgcggtgggg gtgccgcacc cggactatgg
    15001 ccaggatatc gccgtctacc tggtgctgcg cgacaacgcg gttttcgaca gcgacgcgtt
    15061 gcgccgccat tgcctgagcg agctgggcaa gtacaagaca cccagcaagt atgtcgtggt
    15121 cgacgagctg cctcgcggac cgtcgggcaa agtgcagcgc ctcaagttgc tcgagctgga
    15181 cgcatgagcg attcaagatt tcattgatca cgagaatgcc cacgttggac gtgcgtggag
    15241 ggagggtttt taacaatcca taacgtgcag taaagccaca gagttcgctt acccgacaca
    15301 atctgaccag gaccgacgca gacggccatg tgccgctagt gacggccgag gagacaaaca
    15361 tgcaacgtcg agtgaaaacc catcatttga tattgatggt gatgtgcctg ctgtatttca
    15421 tctcgtatat agaccgcgtc aacatttcgg tggcggcgcc gttgatccgg gacgaaatgg
    15481 gcttgagcaa cctggagctg gggcttgtgt tctcggcctt cgcctacccc tacgccgcca
    15541 tgcagatctt cggcggctgg ttggccgacc gcttcggccc gcgcaaagtg ctcatcacct
    15601 tgtcgctgat ctggggcgcg gcgactgtgc tgaccggctt tgtggggagc gtcattgcgc
    15661 tgctggtcat gcggttcgcg ctgggcatag gcgaaggcgg ggcatttccg acggcgacgc
    15721 gcgccttcac ctactggatg cccgtgacgc agcgtggcgt tgcccagggc attacccatt
    15781 cgttctcccg cctgggcggc gccatcacac cacccttggt ggtcctgatc atcgccttgg
    15841 caggctggcg cgagtccttc atcgtgctgg gcgccatcag cctggtttgg accgttctgt
    15901 tcatcttcgt gttccgcgac aacccggccg agcatccgcg cgtcaccccg gctgaactgg
    15961 cggaaatcaa cgccggcagg gtcgagcagc cgaagaaaga gaagggtccg acgccatggg
    16021 ggcggctgat caagggcatg tggaaggtga cgttcgtcga cttctgctat ggctggtcgc
    16081 tgtgggtcta cctgacgtgg ttgccgtctt acttgaagga tgcgcgcggc ttcgacctga
    16141 aagccatggc gattttcacc gccttgccct tgctcgccgg cgttgtggga gatactctgg
    16201 gcggcgtcgt gtccgacaag ttgttgagga agaccggaaa tatccgcttc gcgcgcgtgt
    16261 ccgtcctgac cttcgggctg gtgggcgcgc tgttgtgcat ggtgcccatg gtttccatga
    16321 aagacccgct catggcggtg ttgctcctgt cgctgtcatt cttctgcctg gaactgacca
    16381 atgccgtgct gtggagtctg cccattgata tcgccggcga ttatgcgggc accgcggctg
    16441 gcatgatgaa taccggcttt ggtgtggcgg gaatgatctc gccggtggtg tttggcttcc
    16501 tgatccagag cacgggcagc taccacttgc ccttcctggt ttccgccggc ctgctgggag
    16561 tcggcgcgtt gacggcgctg ctgatcgatc caggccagac cgtgaaacgc gagccggatg
    16621 ttcagggcga gggcaggcgc ttcgagaaga tgggcggtga caccagcctg ggcgtggaat
    16681 agggaggagg gcggtgccat caggtggggc gtccgttccg ttgtttatgg ggtagggtcg
    16741 agtccagcgc gctccaaaaa caaaaacgcc aatcttgcga aagattggcg tttgcaattg
    16801 ataccacata gaaattcttg gtggcgcatc cctgattcga acaggggacc tgcggattat
    16861 gattccgtcg ctctaaccgg ctgagctaat gcgccaaagg acggcactat aacataaaat
    16921 ttccgatgca ccgcgcgaaa gccagaggcc caaaacgcta aaggccccgc cttttggcgg
    16981 ggccttactg ccacgatggc agtgaacact acaagggagt agtgttggcg atctgccact
    17041 gaagggcagt cgggccaact ctttcgagaa ggcaattcca cgatagatca tatcgcatcg
    17101 aatggcaaat cagggcaatt acctaatcgt gcaacaagcc gtaaacgcaa gagaccggtt
    17161 gccttgaggc ttgggtttgt ctgtcacaaa tcgaacgact ttgcagcgaa ccattcgtcc
    17221 atgtggtggg cggatgcccg cgtggtgaca cgcttgccga caatgccctc actctgatga
    17281 agtggcggaa tcccgcccgg tttccggcag cgcctgtctg atgtagccgg cgaacagctg
    17341 tgccgccgcg ggcatggtat cgccattcca caggcaaagt gtctcggata ccgcgctcac
    17401 gccgtccagc cgtgcgcaag ccacgcgccg accgcacgcc agcgcgatgg aacgtggcac
    17461 cagggcgatg cccatgcctt tttccaccat gccgaccacg gtctgccata ggcggacctc
    17521 gtgtttgata cgcgggctga atcccgcctc aacacacagc gcgatgatct ggtcgtggta
    17581 atagggggaa acgcggcggg gaaagaggat gaacgcatcg tcgcgcaggt ctttgacaga
    17641 aaggctggcg cgctcggcca aaggatggcg gagcggcaga cagcacagga acggttccac
    17701 ggccagcctt tcggcccgga tggaggcggg aaaccgcgcc gaatgcgaca accccatgtc
    17761 aagctggttg cgcagcaggg cgtcgatctg ctcggtggta ttcatttccg acagcgtgac
    17821 gtcgatggtc gggtgatccg cttcaaagcg cttgagcgct tcgggcagtc cgcggtggat
    17881 cagggaattc acgaagccga tgcgcaagcg gcccatgatt ccgcgcgccg acatcaaggt
    17941 caggtggcgg gcctcctcgg cctgttgcag caggcgcttg gcttcgcgca acaacacctg
    18001 cccggcgttg gtcagcgtga cagccttgct gctgcgttcc agcagttgca cgtccagttc
    18061 ctgttccaga cggcggatat cggcgctcag ggcgggttgg gagatacaca gcttccgcgc
    18121 ggcacgcccg aagtgcaatt cttcggcgac gcacgagaag tagcggagtt gtcgcaagtc
    18181 catcgatgta tcccccgtca ttccccgcag gagcgccgcg attgtttgaa taattaaata
    18241 ttatcgttaa ttccataaat tgtattggaa cttattgagt aaggttccta gaattttcag
    18301 gaaacgcctt agaaagtcga gaatcatcag gccggcatga ctaccggaca tccaggagaa
    18361 cccttcatga gccaagccgc atccgcgaaa cccagcgcca ttcccgatag ccgtggcacc
    18421 aatctgttcg atcgggatcc cgcctttgac gcgttgttgc ggctttactt gcccgaagcg
    18481 ctgcatgcgc atctgcttcc catattgcgg cggctcggac ggcaggcggg tggtgagctg
    18541 gaccgcctgg cggccagcgc cgacaggaac ccgccggtgc tggcaccacg cacacgcggc
    18601 ggcgccgaca tcgattccat cgaaaaacat cccgactatg tggcgttgga acgcgtcgca
    18661 tactcgaaac tcggactggc cgccatgagc caccgggatg gcgtgctggg ctggtccgag
    18721 ccgctgcccg ctgtggccaa gtacgcgctg acgttcctct tcgtgcaggc cgagttcggc
    18781 ctgtgctgcc cggtcagcat gacggattcc ctgacgcgca cattgcgcaa attcggtgcc
    18841 ccggatctgg tggcgcgttt cctgccgcaa ctgacgtcgc aggatttcga ccagttgtac
    18901 caaggcgcca tgttcatgac ggagcaggac gccgggtccg atgtcggggg cgtcgcgacc
    18961 cgtgcccacc aggatggcgg ccaatggcgc ctgaccggtg acaagtggtt ctgctcgaac
    19021 accgacgcgg aactcgccat ggtgctggcc cgcccggatg gggcgcccaa aggcatcaag
    19081 ggcctgggcc tttttctgtt gccgcgcacc ttgccggatg gcagccgcaa tcactatcgc
    19141 atcatccgcc tcaaggacaa actcggtacc cgttccatgg ccagcggcga gatacggctg
    19201 gacggcgcca tcgcgtatcc ggtgggcgat ctcacgcgtg gtttcaaaca aatggccgac
    19261 atgatcaaca tgtcgcgcct gtccaatggc gtgcgctcgg ccggcctgat gcgtcgcgcc
    19321 tacagcgagg cgcactacgt cgccagccac cgcgaggcgt tcggcaagcg cctgatggac
    19381 atgccgctga tgcggcggca actgatcaag atgctgcttc cgggcgagca ggcgcgttcg
    19441 atgttcctgc ataccgccca gtgtctggcc cagtctgatg cgggcgatgc gcgcgccgga
    19501 aagttgttgc gcatcatgac gccgctgatc aagttccgtg cctgtcgcga cgcccgcaag
    19561 gtgacgggtg atgcgctgga ggtgcggggc ggttgcggct atatcgaaga gtgggtggat
    19621 gcgcggctgg tgcgggattc gcatctgggc tctgtctggg aaggcaccag caacatcgtt
    19681 gcgctggatg tcatgcgcgc ggccacgcgc gaggacgcct tgacgccgtt gcgcgtgtac
    19741 ctggaggaat tgctcgacgc gcccggcatc ccggcggaaa gccgggtgtt gctccaggcg
    19801 ctgttgagtc gcgcggcggg tctggcggat gccgtggcgc gcgatgcccg ggaacatctg
    19861 gcgcgacaag cggccagcgc gctctatcac gtcacaagcg ccatcatcat ggcctgggaa
    19921 gccgcgcgcc aggcacccga ctggcgccgt ctggcgttgg cgcatctggt cgccagattc
    19981 cggctgtcgc ccgtggatcc gctggacgac ggcgatgccg atgcgctgga tccgctgatg
    20041 cgcgccttgc tggcggacga acccatttcg gccgctctgg cgcgagattg tctaacgacg
    20101 tcttccgctc acgctgagac gcgggcggac gaaccctctc tttccctgtg aacggatgga
    20161 acccttcatg aacagccatg gcgccttgca gggcatcaaa gtcatcgacc tcacccgcgt
    20221 gctcggaggg ccttactgca cgcaactcct cgcggaccac ggggccgatg tgatcaagat
    20281 cgagccgccc aagggcgacg agacacgggc ctgggggccc cccttcgacg acgacgggac
    20341 ggccgcgtac ttccgggggg tcaatcgcaa caagcgcggt atcgttctgg acctgacgac
    20401 cgagtccgat cgcgccgttt tgctgcgcct cctggaaggc gcggatgtgc tggtcgagaa
    20461 tttcaagagc ggcacgctgg agcgttgggg gttgggctat gccgaggtgc tggccgagcg
    20521 ctttccgcgc ctgatccatt gcacgatctc cggtttcggc accgacggtc ccctgggcgg
    20581 actgcctggc tacgatgcgg tcatccaggc catggcgggc ctgatgagcg tcaatggcga
    20641 ggatggtggc gggccactgc gtgtgggcct gccggtggtc gacatggtga ccgggctgaa
    20701 cgctgccaac ggcatcatgc tggcgctgca ggagcgccac gtcagcggtc gggggcagtc
    20761 ggtcgaggtg gccttgtacg acgcgggcat ttcgctgctg catccccaca tgcccaatta
    20821 tctggcatcg ggaaagacgc catcgcgcac cggcaatgcc catccgaaca tcacgccgta
    20881 cgacacgtac cgcactggca cggtgcccct gttcctggcg gtcggcaatg attcgcagtt
    20941 ccgggccttg tgccgttatc tcgaagcgcc agaactggct gacgatccgc gtttcgacag
    21001 caatgcgaag cgctgcgggc accggccgga acttaagcgc gagctcgagg cccggttcgc
    21061 ggggcacgat ggcgtggcgc tggcgtcgga cttgattcga cacggtgtgc cttgcggacc
    21121 gttgcacgac gtcgcccagg tggccgatca cccccataca cggcatcgcg gcatgatcgt
    21181 cgccatcgac gattaccgcg gtgtcgcgtc ccccatcaag ctgtcgcgct ccccggccac
    21241 gtatcggcgc aagccgccgc gcttcggtgc cgacacccag gccgtgctgg ccgagctcga
    21301 tggggatcgt tcttgatcct tcattcgtga tgcaggcagg taatagaaat gccggaccag
    21361 aaatgccgga ctgcgacgtg gtccggcgac aacccaagga gactcatgat gttgcgcaga
    21421 attttttcgt ggctgtcggc cgcgt
//
LOCUS       DASTNK010000052         2344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_778589, whole genome shotgun sequence.
ACCESSION   DASTNK010000052 DASTNK010000000
VERSION     DASTNK010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778589"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="VFR20_04005"
     CDS             complement(<1..1256)
                     /locus_tag="VFR20_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEU0229710.1"
                     /translation="MGKITGFLEFDRIAEAAEAPLHRVKHWNEFNKALTDEQASRQGA
                     RCMDCGIPFCSSGCPVNNIIPDWNDLVYKGDWKKALEVLHSTNNFPDFTGRVCPAPCE
                     AACTLNINNDPVGIKSIEHAIIDKGWDNGWVTPQPPARKTGKTVAIVGSGPAGLACAQ
                     QLARAGHHVSVFEKSNRVGGLLRYGIPDFKLDKTHIDRRVAQLEAEGVEFHPSTYVGN
                     ADQAEDGLTVRTPESLVAEFDAVVLTGGAENPRDLPVPGRDLKGIHFAMDFLRQQNMV
                     LKGERIPRQISAKGKHVVVIGGGDTGSDCVGTSNRQGAASVTQIELMPKPPEVEDKDL
                     TWPYWPLKLRTSSSHQEGCERDWAIVTDAFEGKGGKVEKLKAARVEWRKDPATGQARM
                     QKVEGGEFDLQADLVLLAMGFVSPVKP"
     gene            complement(1275..>2344)
                     /locus_tag="VFR20_04010"
     CDS             complement(1275..>2344)
                     /locus_tag="VFR20_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HEU0229711.1"
                     /translation="RIMAQLGIRTFDELVGRADLLDTRAGIAHWKAKGLDFSRVFHQV
                     DPAAPQFQTEEQDHGLDQALDHLLIERSRAAIERGEKVSFITPVRNRNRTIGAMLSGQ
                     VARHHGHEGLPDDTIHIQCNGTAGQSFGAFLAHGITLDLVGEANDYVGKGLSGGRIIV
                     RSPNDFRGFGPDHIIVGNTVLYGALAGEAYFNGVAGERFAVRNSGAAAVVEGTGDHGC
                     EYMTGGTVVVLGDTGRNFAAGMSGGVAYVWDPKKTFRKRCNTAMVDLEPVLSHAEQTQ
                     AGDVENWHSSRPHGERETDETILRRLIEDHFRHTGSFRSRDILGDWEHARTTFVKIIP
                     TDYRRAMAELWRNANLQQVAA"
BASE COUNT          357 a          778 c          762 g          447 t
ORIGIN      
        1 ggtttcacgg gagacacaaa gcccatcgcc agcaagacca ggtcagcctg cagatcgaac
       61 tcgccgcctt caaccttctg catcctggcc tggcccgtgg ccgggtcctt gcgccactca
      121 acgcgcgccg ccttcaactt ctcgaccttc ccgcccttcc cttcgaacgc gtccgtgaca
      181 atggcccagt cacgctcgca gccttcctgg tgcgacgacg acgtgcgcag cttcagcggc
      241 cagtacggcc atgtcaggtc cttgtcctcc acttccggcg gcttgggcat gagctcgatc
      301 tgggtgaccg acgcggcgcc ctggcgattg ctggtgccca cgcaatccga acccgtgtcg
      361 ccaccgccga tgaccaccac atgcttgccc ttggcgctga tctggcgcgg aatgcgctcg
      421 cccttgagga ccatgttctg ctggcgcagg aaatccatgg cgaaatgaat gcctttcagg
      481 tcgcgccccg gcactggcag gtcgcggggg ttctccgcgc cgcccgtcag caccacggca
      541 tcgaactcgg cgaccagcga ttccggcgtg cgcacggtca ggccatcctc ggcctggtcg
      601 gcgttaccca cgtaggtgga cgggtggaat tccacccctt cggcctccag ttgcgccaca
      661 cgccggtcga tatgggtctt gtccagcttg aaatcgggaa tgccatagcg cagcaggccg
      721 cccacgcggt tgcttttctc gaacaccgac acatggtggc cggcgcgcgc caattgctgg
      781 gcgcaggcca ggccggccgg gcccgagccc acgatggcca cggtcttgcc ggtcttgcgc
      841 gctggaggct ggggcgtgac ccagccgttg tcccaaccct tgtcgatgat ggcgtgctcg
      901 atggacttga tgcccaccgg gtcgttgttg atgttcaggg tacaggcggc ctcgcagggc
      961 gcggggcaca cccggccggt gaagtcgggg aagttgttgg tggagtgcag gacttccagg
     1021 gccttcttcc agtcaccctt gtagaccaga tcgttccagt cggggatgat gttgttcacc
     1081 ggacagccgc tcgagcagaa cgggatgccg cagtccatgc agcgcgcgcc ctggcggctg
     1141 gcctgttcgt cggtgagcgc cttgttgaat tcgttccagt gcttgacccg gtgcagcggc
     1201 gcctcggcgg cctcggcgat gcggtcgaat tccagaaaac cagtgatctt gcccatgtca
     1261 ggtatgttcc ttgcttacgc tgcgacttgt tgcaggttgg cgttgcgcca gagttcggcc
     1321 atcgcgcgcc ggtagtcggt cggaatgatc ttcacgaacg tcgtgcgcgc gtgctcccaa
     1381 tcgcccagaa tgtcgcgtga acggaagctg ccggtgtgac ggaagtggtc ttcgatcagg
     1441 cggcgcagaa tggtttcgtc ggtttcacgc tcgccatgcg gccgcgagct gtgccagttc
     1501 tcgacgtcac cggcctgtgt ctgttcggca tgcgacagca ctggttccag atccaccatg
     1561 gcggtgttgc agcgcttgcg gaaggtcttc ttggggtccc acacgtaggc cacgccgccg
     1621 gacatgccgg ccgcgaagtt gcgcccggtg tcgcccagca ccaccacggt gccgccggtc
     1681 atgtattcgc aaccgtggtc gccggtgccc tcgacgaccg ccgcggcgcc cgagttgcgc
     1741 accgcgaagc gttcgccagc cacgccgttg aaatacgctt caccggccag ggcgccgtac
     1801 agcaccgtgt tgcccacgat gatgtggtcc ggcccgaagc cgcggaagtc gttgggcgag
     1861 cgcacgatga tgcgtccacc ggacaagccc ttgccgacat agtcgttggc ttcgccgacc
     1921 aggtcgagcg tgatgccatg cgccaggaac gcgccgaagc tctgccccgc cgtgccgttg
     1981 cactggatgt ggatggtgtc gtcgggcagg ccctcgtggc catggtggcg cgccacctgg
     2041 cccgatagca tggcgccgat ggtgcggttg cggttgcgca ccggcgtgat gaacgacact
     2101 ttctcgccgc gttcgatcgc cgcgcggctg cgctcgatga gcaggtggtc gagcgcctgg
     2161 tccagaccgt gatcctgttc ctcggtctga aactggggcg cggcgggatc gacctgatgg
     2221 aaaacacgcg agaaatccag ccccttcgcc ttccagtgcg cgatgccggc gcgcgtgtcg
     2281 agcaggtcgg cacggccgac cagctcgtcg aacgtgcgga tgcccaactg cgccatgatc
     2341 cggc
//
LOCUS       DASTNK010000053        12753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_410117, whole genome shotgun sequence.
ACCESSION   DASTNK010000053 DASTNK010000000
VERSION     DASTNK010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12753
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410117"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..443
                     /locus_tag="VFR20_04015"
     CDS             3..443
                     /locus_tag="VFR20_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEU0229712.1"
                     /translation="MANLREHAARGAGALVTFTGYVRDYEEGAPTRSLYLEHYPGMCE
                     QEIQALCETATARWAVREPVVVHRVGELHHDEQIVFVAVASAHRGDAFKACEYIIDAL
                     KTRAPFWKRETLEDGKRFWVKQHDADAEKTASWNTPDPQQEQRS"
     gene            440..952
                     /gene="moaB"
                     /locus_tag="VFR20_04020"
     CDS             440..952
                     /gene="moaB"
                     /locus_tag="VFR20_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEU0229713.1"
                     /translation="MKASTTQPKTSLACAVLTVSDTRTAENDTSGDYLQDGLAAEGHQ
                     CVARAISAGNLYQIRKIISDWIANDSIQVILVNGGTGFSHQKATGAAITPLLDTTITG
                     FGELFRHLSYLDIGTSTLQSDAFAGAANDTLIFCMPGSTSACRTAWESIIREQLDSTH
                     RPCNFASHYA"
     gene            1042..2247
                     /gene="glp"
                     /locus_tag="VFR20_04025"
     CDS             1042..2247
                     /gene="glp"
                     /locus_tag="VFR20_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEU0229714.1"
                     /translation="MLEFDEAQQRLSEAGSYPTRTEAISLGLAEGRVLSRPITATVDL
                     PPADNSAMDGYAIRHADAKPGGPLAIQQRCYAGQMPEALRPGHAIRLFTGSLVPANAD
                     TVVMQEDCEEANGAVVVHQAPAIGAHIRRRGEDITAGKELFAAGTRLGAAEIALIASQ
                     GLATIEVWPALRVGILTTGDELVAPGTPRRQEQIYNSNGPMLATLAHGMGVQVKHVLH
                     ALDTPESLDQAFSTLLADCDLILSVGGVSVGEKDLVKPAIEHAGGTLDMWRVRMKPGK
                     PVALAHAQGKPIVCLPGNPVSAFAVFTLLVTPLIRTMQGRHTVMPPVRYGTLDAGRDF
                     DETREEFLRVRFEPSRQGPGTLIPYAHQGSGIISSLPWAHGLARIPANTRVAAGEIVA
                     YYDFSHWLV"
     gene            complement(2377..2994)
                     /gene="mobA"
                     /locus_tag="VFR20_04030"
     CDS             complement(2377..2994)
                     /gene="mobA"
                     /locus_tag="VFR20_04030"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HEU0229715.1"
                     /translation="MIPRHALGGLILAGGQARRLQEPGRTELDKGLLELHGMSLAERA
                     ARYLSSRVSRLWISANRHADIYQRFGDVVADDPDYGVGSGPLSGVASVLAVASTPWLV
                     VIPVDVPELPIDLVDRLADAALASRAGVAYAENAQGAHPLCMVLHRRVLAPLRAHLLD
                     GERKVRRWQQGQGATAVWFEEEGAAFFNINTREDLCVMRRRAPLE"
     gene            complement(2991..4124)
                     /gene="moaA"
                     /locus_tag="VFR20_04035"
     CDS             complement(2991..4124)
                     /gene="moaA"
                     /locus_tag="VFR20_04035"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEU0229716.1"
                     /translation="MSKVTFLDSDVPGAHRPARVSPRVRPAAGLAPEAAGTSPAPHDQ
                     RGRPMHDLRISVTDRCNFRCTYCMPREVFGGNYQFMPHAELLTFEEISRVAGIAVGLG
                     VRKIRLTGGEPLLRKNIEVLVGMLAGLRAPNGEPVDITLTTNGTLLAKKAAALKDAGL
                     KRVTVSLDALDETLFERMSDSQVSVKTVLRGIDEAARVGLTPVKINMVVKKGVNEDQI
                     VPMARHFRHSGHILRFIEFMDVGTSNGWNLKQVVTGAEILARIGQAYPLTPADSSYRG
                     EVASRWRYADDGGEIGLITSVSQPFCGDCTRLRLSPEGKLFLCLFASQGHDLRAMLRG
                     RATDEQISSRLSGVWAARDDHYSEQRGRETVLRQKIEMSYIGG"
     gene            complement(4148..5254)
                     /locus_tag="VFR20_04040"
     CDS             complement(4148..5254)
                     /locus_tag="VFR20_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU0229717.1"
                     /translation="MTCDFKMRLDSQWVLEHGNGEQLVLAETIRLLGAIEATGHIAGA
                     CRACGVSYRHAWGVLRHAEQEFGVPLLETGRRKGTKLTPFGQRLLWADRRAAARLMPT
                     FESMASELQEELENFCPGVHPRLHLHASHGFAVEGMMNVANRLEFPLLELRYRTATEA
                     LASLQRGECDLAGFEVPVGEFETPFLRQYAEWLDPARHCLIHLAVRSIGMFVQPDNPK
                     NIHTLVDLTKPNVRFVNRQFGSSTRYLVGLMLRKLGVETSQIRGFDSSEFTHMAIAAH
                     VVSGMADVGIGVETAAWRSGLSFIPLVKERYFFAVQRDRLSTALMENLFTLMYGETYQ
                     AYLADLVGYDATHAGRVQTLEEAFGSDFALARRA"
     gene            5532..6038
                     /locus_tag="VFR20_04045"
     CDS             5532..6038
                     /locus_tag="VFR20_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEU0229718.1"
                     /translation="MQNSDSEQVCHSSDSTAQQVARQALDHFSGQKGSLLPILHAIQH
                     ALGYVPDAVVPIVAQGLQLSRAEIHGVISFYAHFRSKPPGRVVLQICRAESCQAVGGE
                     HLAAHARQKLGCDFHQTSADGGVTLEPVYCLGLCAQSPAVMLDGEPHARMTPARLDQL
                     LAAKEAQA"
     gene            6035..7612
                     /locus_tag="VFR20_04050"
     CDS             6035..7612
                     /locus_tag="VFR20_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEU0229719.1"
                     /translation="MSAQPITLYIPRDSAAIAVGANDVAQAVEHEAAKRGIALRIVRN
                     GSRGLLWLEPMVEVDTPQGRIAYGPVQASDVNALFDADWLHGGQHPLSQGLTEDIPFL
                     KKQERLTFARVGITDPLDLDDYQRHGGLAGLKQALAMAPGAIVEQVTQSGLRGRGGAA
                     FPTGIKWKTVLGAQAAQKYIVCNADEGDSGTFSDRMLMEGDPYALIEGMAIAGLAVGA
                     TYGYIYVRSEYPYAIATLNDAIARARVAGWLGDSVGGSGKAFDLEVRQGAGAYICGEE
                     TSLLDSLEGKRGMVRAKPPLPAIKGLFGQPTVINNVISLASVPIILAKGAKHYADYGM
                     GRSKGTLPFQLAGNVKQGGLVEKAFGLTLRELLFDYGGGSASGRPLRAVQVGGPLGAY
                     LPESQWDVPLDYEAYIAISAMLGHGGVVAFDDTVDMAKMAQYAMEFCAVESCGKCTPC
                     RIGSVRGTEVIARIRQNEKPEEQAELLRDLCDTMLNGSLCALGGMTPYPVLSALEHFP
                     EDFGLPSPDKTATRKVA"
     gene            7627..10494
                     /gene="fdhF"
                     /locus_tag="VFR20_04055"
     CDS             7627..10494
                     /gene="fdhF"
                     /locus_tag="VFR20_04055"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEU0229720.1"
                     /translation="MLETIVKRERDFGTPASPSANLVELTIDGIAVMAPEGSSVMRAA
                     AEAGINIPKLCATDSQEAFGSCRLCLVQIKDQKGYPASCTTPVRAGMVVWTQTPKLQA
                     LRRNVMELYVSDHPLNCMTCPSNGDCEFQDMAGVVGLREMRYGTEGVNHFDSLKDQSN
                     PYFTFDPSTCIMCSRCVRACDETQGTFALTVDGMGFASRIVAGQDDGFLASDCVSCGA
                     CVQACPTSALTEKSVIDMGQAEHAILTTCAYCGVGCTFKAEMKGDQVIRMTPWKDGQA
                     NRGHACVKGRFAWGYATHKDRILKPMVRKNISDPWREVGWDEAISYAASEFKRIQKKY
                     GRDSVGAITSSRCTNEETWLVQKLVRAAFGTNNIDTCGRVCHSPTGYGLKTTIGESAG
                     TQAFDSVMYTDVVILLGANPSAAHPVFASRLKRRLRQGAKLIVIDPRATELVDSPHVK
                     AAFHLPVRPGTNTALLTAMAHVIVTEGLVDQRFVDARCEHKAFEEWRDFVAQPENSPE
                     ATEATTGVPAADVRGASRLYATGGNGSIYYGLGVTEHSQGSTAVMGIANLAMATGNIG
                     REGVGVNPLRGQNNVQGSCDMGSFPHELPGYRHLSDDVTRKSFEQDWGVTIQPEPGLR
                     IPNMFDAAVDGTFMGLYCQGEDIAQSDPNTQHVAHALQAMECVVIQDMFLNETAKYAH
                     VFLPGSSFLEKDGTFTNGERRIGRVRRVMAPKGGMADWEVTCALSNALGYPMHYRHPG
                     EIMDEIARLTPTFSGVSFAKIDELGSVQWPCNDAAPEGTPVMHVDEFVRGKGRFMITK
                     YVPSDERSTRKYPLLLTTGRILSQYNAGTQTRRTANVVWHKEDVLEIHPHDAQDRGIN
                     DGDWVAIESRAGETVLRAVVTERIQAGVLHTTFHFPESGANVVTTDSTDWATDCPEYK
                     VTAVQARRVTHPSDWQAHWSRFSDVQQQLLRERERDPAQQA"
     gene            10510..11442
                     /gene="fdhD"
                     /locus_tag="VFR20_04060"
     CDS             10510..11442
                     /gene="fdhD"
                     /locus_tag="VFR20_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEU0229721.1"
                     /translation="MKPASNALPRPSATPDPRNARSGKRGHAPNDDDARQPAAASGAS
                     QQRVMRTGHHGLSHFEIDQIAQEIPVALEYNGISHATLLATPCDLEDLAYGFSFTEGI
                     IRTAHDIYDVAVTEAAAGLVIQLTIAHARLNQLKLRRRALAGRTGCGLCGIESLDEVR
                     RDLPALEPRPARISRRAIVHAIDRLRDLQPLHQATGATHAAGWADAQGDVQHVREDVG
                     RHNALDKLIGHLLRHGIAPGDGIAAISSRASFEMVQKSAAAGIGVIAAVSAPTSFAIQ
                     IADELNVLLAGFARDGRFNLYAHPEFLSHPGEPR"
     gene            11439..11699
                     /locus_tag="VFR20_04065"
     CDS             11439..11699
                     /locus_tag="VFR20_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HEU0229722.1"
                     /translation="MNIDHLIRLANRIGDFFESMPDHAEGIEGIANHIQKFWEPRMRR
                     ALLDYLDTHPEGRTDNAALTPIALEAILLNRRRLTPGARVAG"
     gene            11842..>12753
                     /locus_tag="VFR20_04070"
     CDS             11842..>12753
                     /locus_tag="VFR20_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEU0229723.1"
                     /translation="MSTNPSDHGFFSKERITAKPGFNRWMVPPAALAVHLCIGEAYAF
                     SVFNKPMTQVLGITQSLPEDWKLTTLGWIFSLAITLLGLSAAFAGKWLEDVGPRKAMF
                     VAACCFGGGFLIGALGIAWHQIWLVYLGYGVIGGIGLGIGYVSPVSTLIKWFPDKRGM
                     ATGLAIMGFGGGAFIASPLSVALMSHFQSATSVGVAQTFVVLGILYFISMNIGALTIR
                     IPAPGWKPEGWTPPVTQHAMISQNHVHINTAMKTPQFWLIWWALCLNVTAGIGVIGQA
                     SVMIQESFKGAVTPAAAAGFVGLLSLAN"
BASE COUNT         2492 a         4315 c         3762 g         2184 t
ORIGIN      
        1 tgatcgccaa cctgcgcgaa cacgcggcgc gcggcgcggg cgcgctggtg accttcaccg
       61 gctacgtgcg cgactacgaa gaaggcgccc ccacccgcag cctgtacctc gaacactatc
      121 ccggcatgtg cgagcaggaa atccaggcgt tgtgtgaaac cgccacggcc cgttgggcgg
      181 tgcgcgagcc tgtcgtcgtg caccgcgtgg gcgaactgca ccacgacgaa cagatagtgt
      241 tcgtggccgt ggccagcgcg catcgcggcg acgccttcaa ggcctgcgaa tacatcatcg
      301 acgcgctcaa gacgcgtgca ccattctgga aacgtgaaac cctggaagac ggcaaacgct
      361 tctgggtcaa gcaacacgac gccgacgccg agaaaaccgc aagctggaac accccagatc
      421 cacaacaaga gcaacgatca tgaaagcatc caccacccag cccaagacct ctcttgcatg
      481 cgccgtcctc actgtctcgg atacgcgcac ggccgagaac gacacgtcgg gcgactacct
      541 gcaagatggc ctggctgccg aaggccatca gtgcgtcgcg cgggcgatct ccgcgggcaa
      601 cctctatcag atccgcaaga tcatcagtga ctggatcgcc aacgattcga ttcaggtcat
      661 cctggtcaac ggaggcactg gcttcagtca ccagaaagcc actggtgcgg ccatcacgcc
      721 cttgctcgac accaccatca cgggtttcgg cgaactgttc cggcacctgt cgtatctgga
      781 catcggcacg tccacactgc agtccgacgc cttcgccggt gcggccaacg ataccctgat
      841 cttctgcatg ccgggttcca cgtcagcctg ccgaaccgcc tgggagagca tcatcaggga
      901 acaactcgac agcacgcatc gcccctgcaa cttcgcctcg cactacgcat aagggcccgt
      961 tcacacacaa cggaatcccg caccatgcgc cggccggagc cgtcgcggcg cacatccagc
     1021 agccaccatt tcgcacccac catgctcgaa ttcgacgaag cccagcaacg cctcagtgag
     1081 gcgggatcgt accccactcg caccgaggcc atttccctcg gcctggctga aggccgtgtg
     1141 ctctcccgtc cgatcacggc aacggtggat ctccctcccg ccgacaacag tgccatggat
     1201 ggctatgcca ttcgccacgc cgacgcgaaa cccggcggcc cactcgccat ccagcaacgc
     1261 tgttacgccg gacagatgcc cgaggccctg cggcctggcc atgccatccg ccttttcaca
     1321 ggcagcctgg tgcccgccaa tgccgacacc gtggtcatgc aggaagactg cgaggaagcg
     1381 aacggtgccg ttgtcgttca ccaggctccg gccatcggcg cccatatccg gcgccggggt
     1441 gaagacatca ccgctggcaa agagttgttt gccgccggca cgcggctagg cgccgcggaa
     1501 atcgcgctga tcgcctcaca aggcctggcc accatcgaag tgtggcccgc gcttcgggtt
     1561 ggcatcctga ccacgggcga cgaactggtc gcccccggca caccgcgtcg ccaggagcag
     1621 atctacaact ccaatgggcc gatgctggcg accctcgccc acggaatggg cgttcaagtg
     1681 aagcacgtgc tgcatgcgct ggacacccca gaatcgctgg atcaggcgtt ttcgacgttg
     1741 ctggccgatt gcgacctgat cctctctgtc ggcggcgtgt ccgtcgggga aaaagatctg
     1801 gtcaaacccg ccatcgagca cgcgggcggc acactcgaca tgtggcgcgt gcgcatgaag
     1861 cccgggaaac cggtggcgct tgcccacgcc cagggcaagc ctatcgtgtg cctgccgggc
     1921 aatccggttt ccgccttcgc cgtcttcacg ctgctggtca cgccgctgat acgcaccatg
     1981 cagggccgcc atactgtcat gccacccgtg cgctacggca cactggatgc gggacgcgac
     2041 ttcgacgaaa cccgcgaaga atttcttcgt gttcgattcg agccatcgcg gcagggcccg
     2101 ggcaccctga tcccctatgc gcatcagggc tccggcatca tcagctcgct gccctgggcg
     2161 cacggcctgg cccgcatccc cgccaacacc cgtgtcgccg cgggcgagat cgtcgcctac
     2221 tacgattttt cgcactggct cgtctgatct gcgcatcggt cgccatatgc agcccggggc
     2281 gagttgcgca taccattgcg cagtgccatg cccccgtggc gccgaacgcg aggccggccc
     2341 ccagtgtggc acacaaccac cgcccggccg ttcgccctac tcgagcggcg cccgccggcg
     2401 catcacgcat aaatcttccc gggtattgat gttgaaaaac gcggcaccct cttcctcgaa
     2461 ccacacagcc gtcgctccct ggccctgctg ccagcgtctg acttttcgct caccgtccaa
     2521 caaatgcgcc cgcaacggcg ccaacacacg gcgatgcagc accatgcaaa gcgggtgtgc
     2581 gccctgcgcg ttctcggcgt acgccacgcc ggcgcgggat gcgagcgccg catccgccaa
     2641 acggtcgacc aagtcgattg gaagctccgg gacatcaacg ggaatgacca ccagccaggg
     2701 cgtggaggca accgccagca cgctcgcaac gcccgaaagc gggccgctgc ccacaccata
     2761 atccggatca tccgcgacga cgtcgccgaa acgctgatag atgtcggcat ggcgattcgc
     2821 gctgatccac aaccgggaca cacgcgacga cagatagcgt gcggcccgtt cggccagcga
     2881 catgccgtgc aactccagca ggcccttgtc gagttctgtg cggccgggct cctgcagccg
     2941 cctggcctgc ccccccgcga ggataaggcc gcccaatgcg tgcctgggga tcatccgccg
     3001 atgtagctca tttcgatttt ctgccgcaac acggtttcac ggccgcgctg ttcggaatag
     3061 tgatcgtcgc gcgccgccca gacccctgac aggcgggagg aaatctgctc gtcggtggcg
     3121 cgaccgcgca gcatggcgcg caggtcgtgg ccctgcgaag cgaacaggca aagaaacagt
     3181 ttgccttcgg gggacaggcg caacctcgtg cagtcgccgc agaacggctg ggacacactg
     3241 gtgatgaggc cgatctcgcc accgtcgtcc gcataacgcc agcgcgacgc gacctcgccc
     3301 cggtacgatg agtccgcggg agtcaaggga taggcctgcc cgatgcgcgc cagaatctcg
     3361 gcgcccgtca ccacctgctt gaggttccaa ccgttgctgg tgccgacatc catgaattcg
     3421 atgaagcgca ggatatggcc cgaatgccgg aaatgccgcg ccatgggaac gatctggtct
     3481 tcgttcacgc ccttcttgac caccatgttg atcttgaccg gcgtcaggcc cacgcgcgcg
     3541 gcctcgtcga tgccgcgcag cacggtcttg acactgacct ggctgtcgct catgcgttca
     3601 aacaaggttt catcgagcgc atccaggctg acggtgacgc gcttcagacc cgcgtccttc
     3661 aaggcggccg ccttcttcgc cagaagcgtg ccgttggtgg tgagcgtgat gtcgaccggt
     3721 tcgccgttcg gcgcgcgcag gccggcaagc atgccgacca gcacctcgat gttcttgcgc
     3781 aacagcggtt cacctccggt gagacggatc ttgcgcaccc ccaggccgac ggcgataccc
     3841 gcgacgcggg agatttcctc gaacgtaagc aattcggcgt gcggcatgaa ctggtaattg
     3901 ccaccgaaga cctcgcgcgg catgcaatac gtgcaacgga aattgcagcg gtcggtcacg
     3961 gatatccgca aatcatgcat gggccgacca cgctggtcat gcggcgcggg actcgtgccc
     4021 gccgcctcgg gcgccaggcc tgcagcgggc cgcacacgcg gagacacgcg cgcggggcga
     4081 tgcgcgccag ggacatcgct gtcaaggaac gtgactttgc tcatgcgtca atcataaacc
     4141 gtccgcgtca ggctcgtctg gccagtgcga aatccgatcc gaacgcttct tccagcgtct
     4201 ggaccctgcc cgcatgcgtg gcgtcgtacc cgaccagatc ggccagatat gcctgatagg
     4261 tttcgccgta catcagcgtg aacaggtttt ccatcagcgc ggtcgacaag cggtcacgct
     4321 gaacggcgaa gaagtaacgc tccttgacca aaggaatgaa ggacaagccg ctgcgccacg
     4381 ccgcggtctc gaccccgatg cccacatcgg ccatgccgct cacgacatgg gcggcaatcg
     4441 ccatatgcgt gaattcgctg ctgtcgaaac cacgtatttg ggaggtctcg acacccagtt
     4501 tcctgagcat gaggcccact aggtagcgtg tgctggaccc aaactgccga ttgacgaacc
     4561 gcacgttggg cttggtcagg tcgaccagtg tgtggatgtt cttgggattg tcgggctgca
     4621 cgaacatgcc tatgctgcgc accgccagat gaatgaggca atggcgcgcg gggtccagcc
     4681 actccgcgta ttgccgcagg aagggggttt cgaattcgcc cacgggcact tcgaagccgg
     4741 ccaggtcgca ctcgccccgc tgtaacgacg cgagcgcctc ggtggccgtg cgatagcgca
     4801 attcgagcaa cggaaattcg agccggttgg caacgttcat catgccctcc acggcgaagc
     4861 cgtggctggc gtgcagatgg aggcgcggat gcacacctgg acagaagttc tcgagttctt
     4921 cctgcaactc ggaggccatg ctttcgaaag tgggcatgag ccgggcggcg gcacgacggt
     4981 cggcccacaa caggcgctga ccgaaagggg tgagcttggt gccttttcga cgacctgttt
     5041 ccagcaaggg cacaccgaat tcctgctcgg cgtgacgcag gacgccccag gcgtggcggt
     5101 atgacacgcc acaggcgcgg cacgcaccgg cgatatggcc cgtggcttcg atggccccca
     5161 gcaatcgaat cgtttcagcc agcaccagtt gttccccgtt gccgtgttcc agcacccatt
     5221 gcgaatccag gcgcatcttg aaatcacatg tcattttttg cctattcatc ccgtatacac
     5281 gggttttctc cgagatttag aaattatcca catttatgta tttgaataca taatatgttc
     5341 cattttcaga gaggagaatc ggggttctcc ctaggccggc agcctttata catgcttgtt
     5401 ttttcttatt gaaagaatcc agaacagcta gtatcttgcg aggatcacaa cacccatatt
     5461 caagtaaata taaacatata aaagctcttc caggagctca atcccttcgc catttctcca
     5521 gggacaccgc catgcaaaac tcagactccg agcaagtttg tcattcctcg gacagcaccg
     5581 cgcaacaggt cgcacggcaa gcgctggatc atttctccgg ccagaaagga agtctgctgc
     5641 ccattctgca cgccatccag cacgcactgg ggtatgtccc cgacgcggtg gtacccatcg
     5701 tcgcacaggg ccttcaattg tcgcgagcgg aaatccatgg tgtcatcagc ttctacgcgc
     5761 atttccgcag caagccgccg ggccgcgtgg tgctccagat ctgtcgagcc gaatcctgtc
     5821 aggccgtggg cggcgagcac ctcgccgccc acgccagaca aaagctgggc tgtgatttcc
     5881 accaaaccag cgccgatggc ggtgtcacgc tcgaacccgt ctattgcctg ggtctttgcg
     5941 cacaatcgcc cgccgtgatg ctcgacggtg aaccccacgc ccgcatgaca cccgcgcgcc
     6001 tcgaccagct tctggctgcc aaggaggcgc aagcatgagc gcccaaccca tcaccttgta
     6061 cattccacgt gactcggccg ccatcgccgt cggagcgaac gatgtcgcgc aggctgtcga
     6121 acacgaagcg gcaaagcgcg gcatcgcgct gcgcatcgtg cgcaatgggt cgcgcggcct
     6181 gctgtggctg gagcccatgg tcgaagtcga taccccccaa gggcgcatcg catacgggcc
     6241 ggtccaggcc agtgacgtca acgccctatt cgacgccgac tggctgcatg gcgggcaaca
     6301 cccgctctcc caggggctca ccgaagacat ccctttcctg aagaagcagg aacgcctcac
     6361 gttcgcacga gtcggcatca ccgacccgct ggatctcgac gactaccagc gccacggcgg
     6421 cctggccgga ctgaagcagg cgttggccat ggcacccggg gccatcgtcg agcaagtcac
     6481 ccaatccggc ctgcgcggac gaggtggcgc ggccttcccc accggcatca agtggaaaac
     6541 cgtgctgggc gcccaagccg cgcagaagta catcgtgtgc aacgctgacg aaggcgactc
     6601 gggcacgttc tccgaccgca tgctcatgga aggcgacccg tacgccctga tcgaaggcat
     6661 ggccatcgct ggcctggcgg tcggcgccac ctatggctac atttacgtca ggtccgaata
     6721 cccctacgcc atcgccacac tgaacgacgc catcgcccgg gcgcgcgtag ccggatggct
     6781 gggcgattcg gtcggcggca gcggcaaggc gtttgacctc gaagtgcgcc agggcgccgg
     6841 cgcctacatc tgcggcgagg aaacctcgtt gctcgacagc ctggaaggca agcgcggcat
     6901 ggttcgcgcc aagcctccgc tacccgccat caagggcctg ttcggtcaac ccaccgtcat
     6961 caacaacgtc atctcgctgg catccgtgcc catcatcctg gcaaagggcg caaagcacta
     7021 tgccgattac ggcatggggc gctcaaaagg cacgctcccc tttcagctcg cgggcaacgt
     7081 caagcagggc ggcctggtcg aaaaagcctt cggcctcacc ctgcgcgaac tgctgttcga
     7141 ttacggcggt ggcagcgcct cagggcgccc gctgcgcgct gtgcaagtag gggggccgct
     7201 gggcgcctac ctgccagagt cccaatggga cgttccgctc gactacgagg cctacatcgc
     7261 catttccgcc atgctgggcc atggcggcgt ggtcgcgttc gacgacacgg tcgacatggc
     7321 gaagatggcc cagtacgcga tggaattctg cgccgtcgaa tcatgcggga aatgcacacc
     7381 atgccgcatt ggttcggtgc ggggcacgga agtcattgca cgcatacgcc agaacgaaaa
     7441 acccgaagag caggctgagc tcctgcgcga cctgtgcgac acgatgctga acgggtcgct
     7501 atgcgcactg ggcggcatga cgccgtaccc tgttctgtcg gcgctcgaac atttcccgga
     7561 agacttcggc ctgccttccc ccgacaagac cgcgacccgc aaggtcgcct gaatccggag
     7621 cccgccatgc ttgaaaccat cgtcaaacgc gaacgcgatt tcggcacccc cgccagcccc
     7681 agcgccaacc tggtcgaact cactatagac ggtatcgccg tcatggcgcc cgaaggctcc
     7741 tcggtcatgc gcgcggccgc cgaggccggc atcaacatcc ccaagctttg cgccaccgat
     7801 agccaggaag ccttcgggtc ctgtcgcctg tgcctggtcc agatcaaaga ccagaagggg
     7861 taccccgcct catgcacgac accggtgcgc gcgggcatgg tggtctggac ccagacgccg
     7921 aagctgcagg cgctgcgccg caacgtcatg gagctgtatg tctccgatca cccgctgaac
     7981 tgcatgacat gcccatccaa cggtgactgc gaattccagg acatggccgg cgtcgtcggg
     8041 ctgcgcgaaa tgcgttatgg cacagaaggc gtcaatcatt tcgacagcct caaagaccag
     8101 tccaaccctt atttcacctt cgatccgtcc acctgcatca tgtgcagccg ctgtgtgcgc
     8161 gcctgtgacg aaacccaggg cacgttcgca ctgaccgtgg atggcatggg gttcgcctcg
     8221 cgcatcgtcg cggggcagga cgatggcttc ctggcaagcg actgcgtgtc gtgcggggcc
     8281 tgcgtgcagg cctgccctac ttcggcgctc accgagaagt ccgtcatcga catggggcag
     8341 gccgaacacg ccattctcac cacctgcgcc tactgtgggg tgggctgcac gttcaaggcc
     8401 gagatgaaag gcgatcaggt catacgcatg acgccctgga aagacggcca ggccaaccgc
     8461 ggccatgcct gcgtcaaagg gcgtttcgcc tggggttacg ccacacacaa ggaccgcatt
     8521 ctcaaaccca tggtgcgcaa gaacatctcc gacccgtggc gcgaggttgg ctgggacgag
     8581 gctatttcgt acgcggcgtc ggaattcaag cgcatccaga agaaatacgg acgcgactcg
     8641 gtcggcgcca tcacgtcatc ccgctgcacc aacgaggaaa cctggctggt tcagaaactc
     8701 gtgcgcgcgg ccttcggcac caacaacatc gacacctgtg gccgggtgtg ccattccccc
     8761 acgggctatg gtctgaagac caccatcggc gaatcggcgg gcacccaggc ctttgattcc
     8821 gtgatgtaca ccgacgtggt catcctgctg ggcgccaacc ccagcgccgc gcatcctgtg
     8881 ttcgcctcgc gcctgaagcg tcgcctgcgc cagggcgcca aactgatcgt catcgatccg
     8941 cgtgccaccg aattggtcga cagtccgcat gtgaaggccg ccttccacct gcctgtgcgc
     9001 cccggcacca ataccgcatt gttgaccgcc atggcgcacg ttatcgtcac cgagggcctg
     9061 gttgaccagc gctttgtcga tgcgcgctgc gaacacaaag cgttcgagga atggcgcgac
     9121 ttcgtcgctc aacccgagaa ctccccggaa gccaccgagg ccaccaccgg tgtgcccgct
     9181 gccgacgtgc gtggtgcatc gcgtctgtat gccaccggcg gaaacggctc catctattat
     9241 ggccttgggg tgaccgaaca cagccaggga tcgaccgcgg tcatgggcat cgccaatctg
     9301 gccatggcca ccggcaacat cggccgcgaa ggcgtgggcg tcaacccgct gcgcggccag
     9361 aacaacgtgc aggggtcctg tgacatgggt tcgttcccgc acgaactgcc cggctatcgc
     9421 cacctgtccg acgatgtcac gcgcaagtcg ttcgagcagg actggggcgt gaccatccag
     9481 ccagaacccg ggctgcgcat ccccaatatg ttcgatgccg ccgtggatgg cacgttcatg
     9541 ggcttgtact gccagggcga ggacatcgcc cagtccgacc ccaacacgca acatgtcgca
     9601 catgcgctgc aagccatgga atgcgtcgtc atccaggaca tgttcctgaa cgaaacggcg
     9661 aagtacgccc atgttttcct gccggggtcg tcgttcctgg aaaaagacgg caccttcacc
     9721 aacggcgagc gacgcatcgg gcgtgtgcgc cgggtcatgg cgcccaaagg cggcatggca
     9781 gactgggaag tcacctgcgc gctgtccaat gcgctgggct accccatgca ctacaggcat
     9841 ccaggcgaaa tcatggacga gatcgcccgg ctcaccccca ccttctcggg cgtgtcgttc
     9901 gccaagatcg acgagctggg cagcgtgcag tggccctgca acgatgccgc gcccgagggc
     9961 acgccggtca tgcacgtgga tgaattcgtg cgcggcaagg gccggttcat gatcaccaag
    10021 tacgtgccca gcgatgaacg cagcacacgc aagtacccat tgctcctgac cactggccgg
    10081 atcctctcgc agtacaacgc gggcacgcag acccgtcgca ccgccaatgt ggtctggcac
    10141 aaagaagacg tgctggagat ccatccgcac gacgcgcagg accgaggcat caacgacggc
    10201 gactgggtgg ccatcgaaag ccgggcgggc gaaaccgtgc tgcgcgccgt ggtcaccgaa
    10261 cgcatacagg cgggtgtgct ccacaccacg ttccacttcc ctgaatccgg cgccaacgtc
    10321 gtcacgaccg acagcaccga ttgggcgacc gactgtccgg agtacaaagt cacggcggta
    10381 caggcgcgcc gcgtcacgca cccgtcggat tggcaggcgc actggtctcg tttcagcgac
    10441 gtgcagcagc agttgctgcg tgaacgcgaa cgcgatccgg cacagcaggc ctagagacac
    10501 gaagcgatca tgaaacctgc cagcaatgcc ctgccgagac ccagtgccac cccggatccg
    10561 cgaaacgccc gatccgggaa gcgcggccac gcgccgaacg atgacgacgc ccgtcaaccc
    10621 gccgcggcaa gcggcgccag ccaacagcgg gtcatgcgca cgggccatca cgggctatcg
    10681 catttcgaga tcgaccagat cgcccaggaa atccccgtgg cgctcgaata caacggtatc
    10741 agccatgcca cgctattggc caccccttgc gacctcgaag acctggccta tgggttttcg
    10801 ttcaccgaag gcatcatacg caccgcccac gacatctacg acgtcgccgt cacagaagcg
    10861 gctgcgggct tggtgataca gctcaccatc gcccacgcgc gcctgaacca gctcaagctg
    10921 cggcgccgag ccctggccgg tcgcacaggt tgcgggctgt gcggcatcga gagcctggac
    10981 gaggtgcgcc gcgaccttcc cgcgctcgaa ccacgccccg cgcgtatatc ccgacgcgcc
    11041 attgtccacg ccatcgaccg cctgcgcgac ctccagccgt tgcatcaggc cactggcgcc
    11101 acccatgcgg cgggttgggc ggatgcacaa ggcgatgtcc agcatgtgcg cgaagacgtg
    11161 gggcgccaca acgccctgga caaactcatc ggacatctgc tgcgccatgg catagccccg
    11221 ggtgacggca tcgccgcgat ctcaagccgc gccagcttcg aaatggtgca gaaatcggct
    11281 gcggccggca taggggtcat cgctgccgtg tcggcgccca cttcgttcgc catccagatc
    11341 gccgacgagc tcaatgtcct gcttgccgga ttcgcgcgtg acggacgctt caatctctac
    11401 gcccatccag aattcctcag ccaccccgga gagccgcgtt gaatatcgac catctgatcc
    11461 gcctggccaa tcgcatcggc gactttttcg aatccatgcc cgaccatgcc gaaggcatcg
    11521 aaggcatcgc caaccatatc cagaaattct gggaaccgcg catgcgccgg gcgctgctcg
    11581 attacctgga cacccatccg gaggggcgca cggacaatgc ggcgctgaca cccatcgcac
    11641 tcgaggccat cctgctgaac cggcggcgcc tcacacctgg agcgcgggtt gcgggctgaa
    11701 acccgtcgat cggcattcca tatcaattca gttttccact ctcctggaaa agtccgccac
    11761 agcgggtttc ccaggaggct ttccgcaagc tgtagttgat gcaaaacaag aatacatgtc
    11821 caagcaagag gagactacgc aatgagcacc aacccgtctg atcacggatt tttcagcaag
    11881 gaacgcatca ccgcgaaacc cggcttcaat cgctggatgg ttccgcccgc ggcactggcc
    11941 gtacacctct gcatcggtga ggcctacgct ttctcggtat tcaacaagcc catgacgcaa
    12001 gtcctgggca tcacccagtc cctgccggaa gactggaaac tgacgacctt gggatggatc
    12061 ttcagcctcg ccatcacgct gctggggctt tccgccgcct tcgcgggaaa atggctggaa
    12121 gacgtggggc cgcgcaaagc catgttcgtg gctgcgtgct gtttcggggg tggattcctg
    12181 attggtgcgc tgggcatagc ctggcatcag atctggctgg tgtacctggg ctatggcgtc
    12241 ataggcggta ttggcctggg cattggctac gtttcgccgg tttccactct catcaaatgg
    12301 ttccccgaca agcgcggcat ggcgacaggc ctggccatca tggggttcgg tggcggcgca
    12361 ttcatcgcct caccgctgtc cgtcgccctg atgagccact tccagtcggc caccagcgtg
    12421 ggcgtggccc agaccttcgt cgtcctgggc atcctttact tcatttccat gaacatcggc
    12481 gccctcacca tccgcatccc tgcgccgggc tggaaacccg aaggctggac gccgcccgtc
    12541 acgcagcacg ccatgatcag ccagaaccac gtgcacatca acacggccat gaaaacccca
    12601 cagttctggc tgatctggtg ggcgctgtgc ctgaacgtca cggcgggcat cggcgtcatc
    12661 ggccaggcct cggtcatgat ccaggagagc ttcaagggcg ccgtcacgcc tgctgccgcg
    12721 gccggcttcg tgggcctgct ctccctggcc aac
//
LOCUS       DASTNK010000054        21074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1150701, whole genome shotgun sequence.
ACCESSION   DASTNK010000054 DASTNK010000000
VERSION     DASTNK010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 21074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21074
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150701"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFR20_04075"
     CDS             <1..528
                     /locus_tag="VFR20_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0229724.1"
                     /translation="LEALAGLRPVSNGSIRVLGRPVTAGVDPGLGDGVALLDQRPFFS
                     AASIADNLRMACPDADDARLSRALAQACAADFVAAAPNGMQTCLGMAGHGLSGGQLHR
                     LALARVFLLDPGLVLLDEPAAHLDAETRMRVIDSILAFCARRTLLITTHDPRIAARMG
                     RVLHIERDGGLLERA"
     gene            515..2227
                     /gene="cydC"
                     /locus_tag="VFR20_04080"
     CDS             515..2227
                     /gene="cydC"
                     /locus_tag="VFR20_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HEU0229725.1"
                     /translation="MNALDLLWPWLVRHRRRLACAVLLAAVTVWAGVGLFAVAGWFLT
                     GAFLAGASLAFDLFAPSALVRGLSFLRIGSRYAERVAGHAATLDLLADLRTAVFARVM
                     ALSPAQLARYRDGDLVARLIGDVDTLDTLFLLIVAPALTAIVAGLMFGVLVGLYAPVL
                     AWSVTAALLAGGLLIPWVLARVSRRPGRQVQGEASRARALIHDAVQGHVDIAAFGAQP
                     RVAEQFSVVARRLSGARDRVAALGAWGQLVQQVVSGVGMLLLLWLGLLAFQAGAITGP
                     MWAGIVLGGIGLFEVTAPLMRGSSRTGAMAEGTRRVRALFDTRPVIGQPAQPLDLPES
                     GALVLRDVGYRHDGGHQVFEHVDLRIEPGRRVAITGVSGAGKSTLLSLFLRVMDPTQG
                     QVSYGDVPLPHANLVQLHARIALLSQFSPVFLGSLRDNLRVGDPDASDERLWRVLEQA
                     RLADFVHSLEDGLDAWVGESGHTLSAGQARRLCLARTLLAPASVWVLDEPTAGLDEPT
                     ARAFFHDLASVAGDRTVIMATHADLPEDTFDQVLLLEDGALRAAASECPSRGKFNRLA
                     FMGS"
     gene            2333..2893
                     /locus_tag="VFR20_04085"
     CDS             2333..2893
                     /locus_tag="VFR20_04085"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU0229726.1"
                     /translation="MYTDPAEYIRNTIRSVPDWPKPGVTFRDITPVLQDPRTFRVLID
                     LFVYRYMRHRLDLVAGVDARGFILGAVVAYELNLGFVPVRKKGKLPFTTLAEEYTLEY
                     GNAAVEMHTDAVRPGQRVLLIDDLVATGGTMVAAAKLLQRLGANVVEAAAIIDLPDLG
                     GSQAVQATGLPLYTVCSFGEAAIGNQ"
     gene            complement(2908..4416)
                     /gene="ilvA"
                     /locus_tag="VFR20_04090"
     CDS             complement(2908..4416)
                     /gene="ilvA"
                     /locus_tag="VFR20_04090"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEU0229727.1"
                     /translation="MPIDYLKRILTSKVYDVAVESPLEPAPSISARIQNRVLLKREDT
                     QAVFSFKLRGAYNKMANLGAEELKRGVIAASAGNHAQGVALAAKRLGCRAVIVMPTTT
                     PRVKIDAVRHLGGEVVLAGDSFSDAYLHAKELEEKEKLTFVHPFDDPDVIAGQGTVGM
                     EILRQHPGPIDAIFVAIGGGGLIAGVSAFVKQLRPEIQIIGVQTEDSNAMMRSIKAGR
                     RVMLSDVGLFSDGTAVKQVGVETFRLARQYVDDYIAVDTDAICAGIKDVFQDTRSVLE
                     PAGALALAGAKRYAALHGWKNKTLVAITCGANMNFDRLRFVAERADVGEAREAVFAIT
                     LPEKRGSFRRLCEAVGKRAVTEFNYRISDPDKAHVFVGLAISSPSEPEKIANAFRKKG
                     FPTLDLTLNEMAKTHLRYMVGGHSGLAEHEQLFRFEFPERPGALMKFLSAMNPEWNIS
                     LFHYRNQGDDYGRILVGIQVPPDDKKKFKEFLDTLGYPYWNETENPAYSLFL"
     gene            4602..5201
                     /locus_tag="VFR20_04095"
     CDS             4602..5201
                     /locus_tag="VFR20_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEU0229728.1"
                     /translation="MEAWVHSSVLWLLSVLALPKVGLSAIFIISLVSATLLPLGSEPA
                     VFAFVKVAPDMFWPAILVATVGNTLGGIVSYVMGLGAEKAYEGWRHRQESGASRRHGT
                     HLDAGPGPLASARRARRREVAPSRVGGRWHVHIHRWADRLGPVALLFSWLPVVGDPLC
                     AVAGWLRLSFWPCVFWMAIGKAARYITFTVAFLHLVPFL"
     gene            5313..9254
                     /locus_tag="VFR20_04100"
     CDS             5313..9254
                     /locus_tag="VFR20_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEU0229729.1"
                     /translation="MNAPLASQILASPADSASSSTRLREIPYNYTSFSDREVVCRLLG
                     EDAWQALSELRGERRTGRSARMLFEVLGDIWVVRRNPYLQDDLLDNPRRRRLLVEALE
                     HRLTEIDHRREAGAARDAAVARLLQAARGAVDAFSREFDDIAGLRKKILHRLARITAK
                     DNIRFDGLSRVSHVTDATDWRVEYPFVVLMPDNEGEMAALVRACIELDLTIIPRGGGT
                     GYTGGAIPLTWRSVVINTEKLDGLGAVEATDLPGVEGKVATILTGAGVVTRRVAEAAE
                     AAGQVFAVDPTSADASCVGGNVAMNAGGKKAVLWGTALDNLAWWRMVDPQGDWLEVTR
                     LDHNLGKIHEAAEARFELAWFDGAQAPGAKRLRSEILIVEGARFRKVGLGKDVTDKFL
                     AGLPGVQKEGCDGLITSARWILHRMPPHTRTVCMEFFGQARDAIPSIVEVKQYLDTEG
                     RARGAILAGLEHLDERYLRAVGYATKSKRGVLPKMVLIGDIVGEEADAVATAASEVVR
                     IANTRHGEGFVAVSAEARKKFWLDRSRTAAIARHTNAFKINEDVVIPLARMGEYTDAI
                     ERINIELSTRNKLRLLDQLEAFLGGALPMGKAEDPDDAEHTREEILSDRTRTAIETLQ
                     VIRERWSWLLAGLDLPLADSLADLRRLGVEARAGTFDERLAAQPGARVFDLLQDHTIR
                     ISWKAEVRALMERYFAGTDCAAVLAEIQAIHDRVLKGRVFVALHMHAGDGNVHTNIPV
                     NSDDYDMLREANQAVARIMRIARDLDGVISGEHGIGLTKYEFLTEQELQPFQDYKRRV
                     DPDARFNAGKLMPGADLRRAWTPSFNLLGYESLIMQRSEIGAISNSIKDCLRCGKCKP
                     VCATHVPRANLLYSPRNKILATSLLIEAFLYEEQTRRGVSLQHWAEFEDVADHCTVCH
                     KCYNPCPVDIDFGDVSMEMRALLRRAGKKSFNPGTAASMFFLNAKDPRVIKATRKVMI
                     DLGYKAQRLAHDVLSVPSRKQTAQPPATLGKAPVREQVIHFINKKMPGGLPKQTARKL
                     LDIEDADYVPIIRDPAATSADSEAVFYFPGCGSERLFSQVGLATQAMLWHVGVQTVLP
                     PGYLCCGYPQRANGLTDKAEKIITDNRVLFHRMANTLNYLDIKTVVVSCGTCYDQLAG
                     YEFDKIFPGCRLIDIHEYLLEKGLKLEGVQGVRYMYHDPCHTPMKLQDPMKTVKSLVG
                     DTTIKADRCCGESGTLAVSRPDISTQVRFRKQEELAKNSAAVRADGFGGEVKILTSCP
                     SCLQGLSRYEDDTGMQADYIVVEMARHMLGEDWMRQYVANANAGGIERVLV"
     gene            complement(9289..10629)
                     /gene="phoR"
                     /locus_tag="VFR20_04105"
     CDS             complement(9289..10629)
                     /gene="phoR"
                     /locus_tag="VFR20_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HEU0229730.1"
                     /translation="MIRPAICLVAWAAFAWLCGSWLSREAGWAVFTLGLLAVMAIQHR
                     RLARVSHWAGNVNDPPPPIRGDWADILASVYRALRKNRQDMDLLERQAQGIMQAAEAL
                     PDGVMTLDDALRMTWCNHEAARHLGLDPSADPGHSIFNIVRAPEFARYAKQAHWDGPL
                     LLRVAGDGRDATLQIQLIPYGLNRLLMVTRDITQLEKLETTRKDFVANVSHELRTPLT
                     VLGGFLETLRDLPPEALSNAQRDHYLDLMREQTGRMQTIVEDLLTLSTLESSPPDDGV
                     PVDMDAVIATALAQAQALSGGRHVFEQRIEKGLGVHGNANELSSAVLNLLTNAVRYTP
                     PGGTISVTWERSATGGARYAVRDTGIGIAAQHLPRLTERFYRVDRGRSRATGGTGLGL
                     AITKHVAMRHQASLHMESRPNAGSLFALAFPATRLVTANVAAKNQDAGADGHLK"
     gene            complement(10626..11336)
                     /gene="phoB"
                     /locus_tag="VFR20_04110"
     CDS             complement(10626..11336)
                     /gene="phoB"
                     /locus_tag="VFR20_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEU0229731.1"
                     /translation="MTTTVLVVEDEPAILELISVNLGFAGYQVRSAGDGGQARAMIRD
                     ELPDLILLDWMLPDTQGVALARHLRAQERTRNVPLIMLTAKSAERDKIEGLEAGADDY
                     VTKPFSPKELIARIKAVLRRRAPQHTDDIIDTGSLSLDPASHRVSAGGQSLVLGPTEF
                     RLLHFFMTHAERVFSRTQLLDQVWGDHVFVEERTVDVHIRRLRKALEPAGQDNTVETV
                     RGIGYRFSARPSPAGGTP"
     gene            11469..12257
                     /gene="ubiE"
                     /locus_tag="VFR20_04115"
     CDS             11469..12257
                     /gene="ubiE"
                     /locus_tag="VFR20_04115"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEU0229732.1"
                     /translation="MQHDSSPDSPRPSGTTTHFGYATVGEAEKAGKVAEVFHSVASRY
                     DLMNDLMSAGMHRAWKAFTVARADVRPGMKVLDIAGGTGDLAKAFARRAGANGEVWLT
                     DINDSMLRVGRDRMVDRGLLLPTAVCDAERLPFPDGYFDRVTVAFGLRNMTHKDRALA
                     EMRRVLKLGGKLLVLEFSKVARPLAPVYDWYSFSVLPWLGRKVAGDEGSYRYLAESIR
                     MHPDQQTLAGMLESAGLSRVRFFNLTAGVVALHEGVNLGPNPQA"
     gene            complement(12301..13608)
                     /locus_tag="VFR20_04120"
     CDS             complement(12301..13608)
                     /locus_tag="VFR20_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEU0229733.1"
                     /translation="MASALLLGLTCSRANASAFQLIEQNASGLGNSYAGSAAIADNAS
                     TIFYNPAGMTRLPGVNLSAGMTAITTTFKFSDNGSTGPGGLPLGTNQGGDAGGTSFLP
                     NLYASWQLDPSWFVGLGVGAPYGLSTDWDSDWVGRYQSTRFDLQSVNINPSVAYKASS
                     RLSLGVGLDWLYADADYRRNVPTAGLIPQIPSTLPGSVRQALASQLMASPDMNARARL
                     HGSGWGWNMGLLYRATDQTRIGLAYRSRVHVPLEGTTSLAGIPGAFSSAIPGAVDNSA
                     SATLPDTATLSVVHDINSKWQVLADLAWTGWSSLPELKIHNGALGDDTLKLEFRNTWR
                     VALGANYQINGRWKLRTGVSYDQSPIRGSDLRPTSLPDNDRVSVALGVQYRFNQHATV
                     DVGYEHLFFHSHINNATNPAEGTVTGTLNTAADLVGVQVSYRF"
     gene            14052..16040
                     /gene="pdeR"
                     /locus_tag="VFR20_04125"
     CDS             14052..16040
                     /gene="pdeR"
                     /locus_tag="VFR20_04125"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006758253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HEU0229734.1"
                     /translation="MSDVSDSLLFAYFGTRSPFWQLSADSNALQLAGTQGTPNLAVAL
                     APDKAREIRAFTGVTSHAEIEIELFGEPQKLSLVGKKNGANSWSGTASDYMDTPAVAK
                     DLAHGLEFAEQVISEVNSLVVIMDHNLNILRFNRLCEEVTGLREKDVIGKPAHAFMSM
                     SERDASKANIADFFKNSAAYEVERHINTLNGPRTILWRNKFVQSGSGPDERYLVCSGT
                     DVTEERKAQAKLMELATVDTLTGLPNRHAIYEQINRSLNNGERFGILFLDLDNFKQVN
                     DHYGHLVGDSLIQAVALSIVSCMREGDSVARLGGDEFLVLVKNASFELMENVAQRITD
                     RLKAPISLGLVEVYTGCSIGIALCPEHGDSIDHLVRCADTAMYVAKDSGKRTYRVFSP
                     DMDSRVAEYIWLDSNLRKALDAEDQLQLYYQPKLCLETGEVRSVEALLRWDSPERGRI
                     SPMDFVPYAEESGLIMPLGRWVMRTAAKQAAAWKARGLDMRIAVNLSARQLSSSTLIQ
                     DFSEALRQSDQTRSLLDIELTESCLIHDEAAALRLIQQFRELGAEVHLDDFGTGYSSL
                     SQIARLPLDAIKLDRSFVKSVDENATSQALVRSMVVVAQELKFKVIAEGVETSGEAEF
                     LKDVGVEYTQGYLYGRPMSVKEFEDWLDNKGKRLRLVA"
     gene            complement(16105..16971)
                     /locus_tag="VFR20_04130"
     CDS             complement(16105..16971)
                     /locus_tag="VFR20_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006397815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEU0229735.1"
                     /translation="MRLEDHPSCRLFSEADNLTQITPYYEEGRNVLWMMLNAQPRPCF
                     NTALLHDISRLKRAAQDSGLPIDFWVTGSVVPQIFNVGGDLELFAAHIRAGEHEQLRA
                     YARACIDAIHDAMVGFGTGAITIAMVEGSALGGGFEAALAHHYFLAQRDVKMGFPEIA
                     FNLFPGMGAYSLVARKSHMLLAEELISTGVSHTAEWYQEKRLVDQVFEKGDAFMATRT
                     LIDVIRPKMNGIRAMLRARQRVLSIDRSELMEITEDWAQAAFTLEEKDLQYMERLVML
                     QNRHAKQARNVA"
     gene            complement(17464..17589)
                     /locus_tag="VFR20_04135"
     CDS             complement(17464..17589)
                     /locus_tag="VFR20_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229736.1"
                     /translation="MERSGGQDEKTFEVRFAATAYWTPCHETETDGKLNINLNQS"
     gene            17588..18538
                     /locus_tag="VFR20_04140"
     CDS             17588..18538
                     /locus_tag="VFR20_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEU0229737.1"
                     /translation="MSRFFSKHLVTALLLVSSLGMLTASFNAEARRMGGGGSFGRQSL
                     NVTKQRQAITPPAAKSPAAAAQNSTAGARGGASRWLGPLAGIAAGLGIAALLSHLGLS
                     GAFLETISSMVLIGLVLAAVIFLVRRLLAGARAPASAAAGDAAPGGALRSAWNSGGSP
                     QRPAGAASSSHAPAAMSPSFAASSVPVDGDSNWVIPHDFDSVAFLAQAKKQFAAIQTL
                     WDSGEVNQLAGYLTDDLMAEIAPQVAANQGAKPTEIVLLNAELLGVESVEGGHLASVR
                     YSGMLREDGHTEAFRFQEVWNLYKSAADGWLLAGIQQISA"
     gene            18674..19315
                     /locus_tag="VFR20_04145"
     CDS             18674..19315
                     /locus_tag="VFR20_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEU0229738.1"
                     /translation="MFPIPSFLAPAAVGARVLNALLAREDWARERLARHAGKTVRFVV
                     GHMTVGLVIASDGSVSAGNPSVVPDVTLTIPPERLSELPQALRSSDSERIAGLMHIQG
                     DAALAHGVAGLAHDLRWDVENDLSRLVGDVAARRLVSAFRGLAVGARRGTHRLAANLG
                     EYLAEESAFLSGRDAYVDWAAGVRAAGARLDALENRVARLSRPGPHPGHKGGR"
     gene            19318..20874
                     /gene="ubiB"
                     /locus_tag="VFR20_04150"
     CDS             19318..20874
                     /gene="ubiB"
                     /locus_tag="VFR20_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEU0229739.1"
                     /translation="MPTLFRLIRIIFVALRYRLDELVLSSIKHPLAYGLLRIVRFGRT
                     PRTPRGQRLRLALESLGPIFVKFGQVLSTRRDLIPLDVARELALLQDRVPPFPSDQAA
                     QCVQDALGESPHTLFKSFDADPVASASIAQVHFAVLHDGREVAVKVLRPGMLSIIEKD
                     LRLLRALSGLVERLVPDGRRLKPREVVAEFDKYLHDELDLIREASNCSQLRRNFGPDT
                     ERGNLLMVPEVVWEYCATTVFTMERMRGVPISRVEQLRELGVDIKNLARTGVEIFFTQ
                     VFTDGFFHADMHPGNIYVSDTPETLGRYIALDFGIMGSLSEFDKNYLAQNFLAFFRRD
                     YRRVAQLHLESGWVPENTREEELEGAVRAVCEPYFDRPLSEISLGQVLLRLFQTSRRF
                     NVEIQPQLVLLQKTLLNVEGMGRDLDPSLDLWETAKPYLEKWMRQRVGLGALRVRLAQ
                     EAGQWAQILPQIPRLVHANLVRPDSQAHLHAEVRRMRQEQRQTNRLLSALVAVLAVAI
                     GVAMWALTRS"
BASE COUNT         3649 a         6601 c         6697 g         4127 t
ORIGIN      
        1 ctggaagcgc tggcgggctt gcgtccggta tcgaatggaa gcatccgggt gctgggcagg
       61 cctgtgaccg ccggggtcga tccaggcctg ggggatggcg tcgccctgct tgaccagcgc
      121 ccattctttt ccgcggcctc cattgccgac aacctgcgca tggcctgccc ggacgccgac
      181 gacgcccggt tgtcccgggc cctggcgcaa gcgtgtgccg ccgacttcgt ggccgccgcc
      241 cccaacggaa tgcagacgtg cctgggaatg gcgggtcatg gcttgtcggg gggccagctc
      301 catcgattgg cgctggcccg ggtcttcctg ctggaccctg gcctggtgtt gctggatgag
      361 ccggccgcgc atctggacgc ggagacccgg atgcgggtca tcgattccat cctggcgttc
      421 tgtgcgcggc gcaccttgct gatcaccacg cacgacccgc gcatcgccgc ccgcatgggg
      481 cgcgtgttgc acatcgaacg cgacggaggc ctgcttgaac gcgcttgatc tgttgtggcc
      541 ctggctggtc aggcataggc gccgcctggc ctgcgccgtg ttgctcgccg ctgtcacggt
      601 ctgggcgggt gtgggtctgt tcgccgtggc gggctggttt ctcaccggcg cctttctggc
      661 gggcgcgtcc ctggcattcg acctgttcgc cccctcggcg ttggtgaggg gcctctcttt
      721 cctgcgcatc ggctcccgtt atgccgagcg ggtcgccggc catgccgcca ccctggatct
      781 cctggccgat ttacgcaccg cggtgttcgc ccgtgtcatg gctctgagcc cggcgcagtt
      841 ggcccgctat cgcgacggcg atctggtcgc ccggctgatc ggcgacgtgg acaccctgga
      901 caccttgttc ttgctcatcg tggcccccgc gctgaccgcc atcgtcgccg gtctcatgtt
      961 cggtgtgctg gttggcctgt acgcgccggt gctggcatgg tcggtcaccg ccgcattgct
     1021 ggcaggcggc ctgttgatcc cctgggtgct ggcgcgggtg tcgcgtcgcc ccggcaggca
     1081 ggttcagggc gaagcgtcgc gcgcacgcgc cctcatccat gacgcggtac aggggcatgt
     1141 cgacatcgcg gccttcggtg cccagccacg cgtggctgaa cagttcagtg tggtcgcgcg
     1201 acggctctcc ggcgcgcgcg accgtgtcgc cgccctgggt gcctggggcc aactcgtgca
     1261 acaggtcgtc tcgggcgtcg gcatgctgct gcttctttgg ctgggcctgt tggccttcca
     1321 ggcgggcgcc atcaccggac cgatgtgggc tggcatcgtg ctgggtggca ttggcctgtt
     1381 cgaagtgacc gcccccctga tgcgcgggtc gtcgcgcacc ggtgccatgg ccgagggcac
     1441 gcgccgcgtg cgtgcgctgt tcgacacccg gccggtcatc ggccagcccg cgcaacccct
     1501 ggatctgccc gaatcgggag ccttggtgtt gcgggatgtg ggctatcgtc acgacggagg
     1561 ccaccaagtg ttcgaacatg tggatctgcg catcgaaccg gggcgccgcg ttgcgatcac
     1621 cggtgtcagc ggcgcgggga aatccacgct gctgtccttg tttctgcgtg tcatggatcc
     1681 gacgcagggc caggtgtcgt acggcgatgt gccgttaccg cacgccaatc ttgtccagct
     1741 gcatgcgcgt attgccctgc tctcgcagtt ttcccccgtg ttcctcggct cgttgcgcga
     1801 taacttgcgt gtgggcgatc ccgatgcctc cgatgagcgg ctatggcgtg ttctggagca
     1861 ggctcgcctg gccgatttcg tgcactcgct ggaagacggc ctggacgcct gggtgggcga
     1921 atcggggcac acgctgtccg ccgggcaggc tcgccgtctg tgtcttgcac gcacactgct
     1981 tgcgcccgcc agcgtctggg ttctggatga gcccaccgcc ggcctggacg agcccaccgc
     2041 ccgggcgttc ttccacgatc tcgccagcgt cgcgggtgac cggaccgtca tcatggccac
     2101 ccatgccgac ctccccgaag acactttcga ccaagtgctc ctccttgagg atggcgcgtt
     2161 gcgcgccgcg gcatccgaat gcccgagtcg tgggaagttc aatcgtctgg cgttcatggg
     2221 gtcatgacat cggggcgtgt agaataggcg ctgtgcgcag agcctcccgg tttccgcgta
     2281 gccctcattc ccgggccggc ccgcgtccgg cccgttttca tggcgatccc ccatgtacac
     2341 cgaccccgcc gaatacatcc gcaacacgat ccgcagcgtc cccgattggc ccaaaccggg
     2401 cgtcacgttc cgcgacatca cgccggtcct gcaggatccg cgcaccttcc gggtgttgat
     2461 cgacctgttc gtctatcgct acatgcggca ccgcctggac ctcgtcgcgg gtgtcgatgc
     2521 ccgcggcttc atcctgggcg cggtggtcgc ctatgagctc aacctgggct tcgtcccggt
     2581 gcgcaagaag ggcaagctgc ctttcaccac gctggcggag gaatacaccc tcgaatatgg
     2641 caacgccgcc gtcgaaatgc acaccgacgc cgtccgtccc ggccagcgcg tcctgctcat
     2701 cgatgacctc gtcgccaccg gcggcactat ggtcgccgca gccaagttgc tgcaacgcct
     2761 tggcgccaat gtcgtcgaag ccgccgccat catcgatctg cccgatcttg gcggatccca
     2821 ggccgtccag gccaccggcc tgcccctgta cacggtctgc agcttcggcg aggccgccat
     2881 aggaaaccaa taagtccctc gcaagtccta caaaaacaac gaatacgccg gattctcggt
     2941 ctcgttccag tatggatacc ccagcgtatc caggaactcc ttgaatttct tcttgtcatc
     3001 cggcggcacc tggatcccca ccagaatccg cccgtaatca tccccctgat tccggtaatg
     3061 aaacagactg atgttccact ccggattcat cgcgctcagg aacttcatca acgcgccggg
     3121 ccgctccggg aactcgaaac ggaacaactg ctcatgttcg gccagcccgg aatggccgcc
     3181 caccatgtag cgcagatgcg tcttggccat ctcgttgagc gtcaggtcca gagtcgggaa
     3241 acccttcttg cggaacgcat tcgcgatctt ctccggttcg gacggcgagg aaatggccag
     3301 ccccacgaac acatgcgcct tgtccggatc tgaaatccgg taattgaact ccgtgacggc
     3361 acgcttgccc accgcttcac acagccgccg gaaactgcct cgcttttccg gcagggtgat
     3421 cgcgaacacc gcctcgcgcg cctcacccac gtcggcccgc tccgccacga agcgcaggcg
     3481 gtcgaaattc atgttcgccc cgcaggtgat cgccaccagc gtcttgttct tccatccgtg
     3541 cagcgccgca taccgcttcg ccccggccag cgccagcgcg ccggcgggct ccagcacgct
     3601 gcgtgtgtcc tggaacacgt ccttgatgcc cgcgcagatc gcgtccgtat cgaccgcgat
     3661 gtaatcatcc acgtactggc gcgcaagccg gaatgtttcc acgcccacct gcttgacggc
     3721 cgtgccgtcg gagaacagcc ccacgtcgct gagcatcacg cgccggcccg ccttgatgct
     3781 gcgcatcatc gcgttcgagt cctcggtctg cacgccgatg atctggatct cgggccgcag
     3841 ttgcttgacg aacgccgaca cgcccgcgat caggccgccg ccgccgatcg ccacgaaaat
     3901 ggcgtcgatc ggaccggggt gctggcgcag gatctccatc cccaccgtgc cctggcccgc
     3961 gatgacgtca ggatcgtcga agggatggac gaaggtgagt ttttccttct cctcgagctc
     4021 cttggcgtgc agataggcat ccgagaagct gtcgcccgcc aggaccacct cgccacccag
     4081 gtggcgcaca gcgtcgattt tcacgcgcgg tgtggtggtt ggcatcacga tcacggccct
     4141 gcatcccagg cgcttcgccg cgagcgccac cccttgggcg tgattgcccg ccgacgcggc
     4201 gatcacgcca cgcttgagct cctccgctcc gaggttggcc atcttgttgt aggcgccgcg
     4261 cagcttgaaa ctgaacaccg cctgggtgtc ctcgcgcttg agcagcaccc tgttctgaat
     4321 tcgcgccgat atgctgggag ccggttcgag gggggattcg acggcgacgt cgtagacttt
     4381 ggacgtcagg atacgcttca gataatcgat gggcatggcg gcgaaaacag aaagagcaaa
     4441 ggttctggtg agaagctgaa aagagtacca cagcgccagg cagccggatt ttggcgcacg
     4501 ggtgcgcggt ggatggctcc cggcagcgga ggcacgcggc gcggtggttg gcgtgaagaa
     4561 tccctgggta cactatgacc tgttcatcgg ggtggagcgt catggaagcg tgggtgcact
     4621 cgtctgttct gtggctgctg tcggtgctgg ccttgcccaa ggtcgggttg agcgccattt
     4681 tcatcatcag cctggtctcg gccaccttgt tgccattggg ttccgaaccc gctgtgttcg
     4741 ccttcgtcaa agtggcgccc gacatgttct ggcccgccat cctggtggcc accgtgggca
     4801 acaccctggg cggcatcgtc agttacgtca tggggctggg tgccgagaag gcctacgaag
     4861 gctggcgcca caggcaagaa tcgggagcgt ctcgccggca cggcacgcat ctggatgcgg
     4921 ggcccgggcc gctggcttcg gcacgccgcg ccaggcggcg cgaagtcgca cccagccgtg
     4981 tcgggggacg ttggcacgtt catatccatc gctgggcgga ccgcctgggg cccgtggcgc
     5041 tgctcttttc ctggctgccg gtggtgggtg atccgttgtg cgcggtggct ggctggttgc
     5101 gcctttcgtt ctggccatgc gtattctgga tggcgatagg caaggcggcc cgctacatca
     5161 ctttcacagt cgccttcctg catctcgtac ctttcctgta gcgcatcccg ccggtgtgtg
     5221 cttccggtct ggcacgtgct ccgattcatc gaagaattca ttggcttttc gactacacta
     5281 tctctttaag ggccgctttg tcttcatcct ccatgaatgc cccactagcc agtcagatac
     5341 tcgcttcccc cgccgactcg gcttcctcgt cgacccggct gcgcgaaatc ccctacaact
     5401 acacctcgtt ctccgaccgc gaagtcgtgt gtcggctgct cggtgaagac gcctggcagg
     5461 cgctgtccga gttgcggggc gaacggcgca caggccgctc cgcgcgcatg ctgttcgagg
     5521 tcctgggcga catctgggtc gtgcggcgca atccctacct gcaggacgac ttgcttgata
     5581 atcccaggcg gcggcgcttg ctggtcgagg ctctggagca ccggctgacc gagatcgatc
     5641 accggcgcga agcgggggcg gcccgcgatg cggccgtcgc gcgcctgttg caggcggcgc
     5701 gcggcgcggt cgacgcgttc agccgggaat tcgacgacat cgccgggttg cgcaagaaga
     5761 tcctgcatcg tctggcacgc atcaccgcga aagacaacat ccgcttcgac gggctgtccc
     5821 gtgtgtcgca cgtgaccgat gcgaccgact ggcgcgtgga gtacccgttc gtggtgctca
     5881 tgcccgacaa cgaaggggaa atggcggcgc tggtgcgtgc ctgcatcgaa ctggatctga
     5941 ccatcatccc gcgcggaggc ggcaccggct acacaggtgg cgccatcccg ctcacctggc
     6001 gctcggtggt catcaataca gagaagctcg atggcctggg ggccgtggag gccaccgatc
     6061 tccccggcgt ggagggcaag gtcgccacca tcctgaccgg cgccggcgtg gtgacgcgtc
     6121 gcgtcgccga agccgccgag gcggcgggcc aggtcttcgc ggtcgatccc acctcggcgg
     6181 atgcctcgtg tgtggggggc aatgtcgcca tgaatgccgg cggcaagaag gccgtgctat
     6241 gggggacggc gctggacaat ctggcctggt ggcgcatggt cgatccccag ggcgactggc
     6301 tcgaagtcac gcgcctggac cacaacctgg gcaagattca cgaagccgcc gaggcgcgtt
     6361 tcgaactggc ctggttcgat ggcgcccagg cgcctggcgc aaaacgcctg cgcagcgaaa
     6421 tcctgatcgt ggaaggggcg cgcttccgca aggtcggcct cggcaaggac gtcaccgaca
     6481 agttcctggc cggcctgccc ggtgtccaga aagaaggctg cgatggcctg atcacttccg
     6541 cgcgctggat cctgcaccgc atgccaccgc atacgcgcac ggtgtgcatg gagttcttcg
     6601 gccaggcgcg cgatgccatt ccttccattg tcgaagtcaa gcaatacctc gataccgaag
     6661 gccgtgcgcg cggtgccatc ctggccgggc tcgaacatct ggatgagcgc tatctgagag
     6721 cggtgggcta cgccaccaag agcaaacgcg gcgtcctgcc caagatggtg ctcatcggtg
     6781 atatcgtcgg cgaagaggcc gacgcggtcg cgaccgcggc cagcgaagtc gtgcgtatcg
     6841 ccaacacccg ccacggcgag ggctttgtgg cggtcagcgc cgaggcccgc aagaagttct
     6901 ggctcgaccg gtcccgcacc gccgccatcg cgcgccacac caacgccttc aagatcaatg
     6961 aagacgtggt gatcccgttg gcgcgcatgg gtgaatacac cgatgccatc gaacgcatca
     7021 acatcgagct ctccacccgc aacaagctgc gtctgctgga tcaactggag gccttcctgg
     7081 gcggcgcgct gcccatgggc aaggccgaag accccgacga cgccgagcat acccgcgaag
     7141 agatcctgtc cgaccgcacc cgcacggcca ttgaaaccct gcaggtcatc cgcgagcgct
     7201 ggtcatggct gctggccggc ctcgatctcc ccctcgctga cagtctcgcc gatctgcggc
     7261 ggctgggtgt ggaggcgcgg gcgggtacct tcgacgaacg cctggcggcc cagccgggcg
     7321 ctcgtgtgtt cgatctgctg caggaccaca ccatccgcat ctcctggaag gccgaggttc
     7381 gtgcgctcat ggagcgctat ttcgccggca cggactgtgc cgcggtgctg gccgagatcc
     7441 aggccatcca cgaccgggtg ctgaaagggc gggtgttcgt cgcgctgcac atgcatgcgg
     7501 gcgatggcaa cgtgcacacc aacatcccgg tcaactccga cgactacgac atgctgcgcg
     7561 aggccaacca ggcggtggcc cgcatcatgc ggatcgcacg tgatctggac ggcgtcattt
     7621 ccggcgagca tggcatcggc ctgaccaaat acgaattcct caccgaacaa gagctccagc
     7681 cgttccagga ctacaagcgc cgggtcgacc ccgacgcgcg cttcaatgcc ggcaaactca
     7741 tgccgggcgc cgacctgcgc cgggcctgga cgcccagctt caacctgctg ggctacgaat
     7801 cgctgatcat gcagcgtagt gaaatcggcg ccatctccaa ctccatcaag gattgcctgc
     7861 gctgcggtaa gtgcaaaccg gtctgcgcca cgcatgttcc gcgcgccaac ttgctgtatt
     7921 cgccgcgcaa caagatactg gccacgtcgc tgctgatcga agccttcctc tatgaagagc
     7981 agacccggcg cggggtcagc ctgcaacact gggctgaatt cgaagacgtg gccgatcatt
     8041 gcaccgtttg ccacaagtgc tacaacccct gtccggtgga cattgatttc ggcgatgtct
     8101 cgatggagat gcgcgcgctg ctgcgacgcg cgggcaagaa atcgttcaac cccggcacag
     8161 cggcgtcgat gttcttcctc aatgccaagg atccgcgcgt gatcaaggcc acgcgcaagg
     8221 tcatgataga cctggggtac aaggcgcagc gcctggccca cgacgtgctg tcggtgccat
     8281 cgcgcaagca gaccgcgcaa ccgcccgcca cgttgggcaa ggcacccgtg cgcgagcagg
     8341 tcattcattt catcaacaag aaaatgcccg gcgggctgcc caagcagacc gcgcgcaaac
     8401 tgctggacat cgaagacgcc gactacgtgc cgatcatccg cgatccggcg gccacctccg
     8461 cagacagcga ggcggtgttc tactttcccg gctgcggctc cgagcgcctg ttctcgcaag
     8521 tcggcctggc cacccaggcc atgctttggc atgtgggggt gcaaacggtg ttgcccccgg
     8581 gctatctgtg ctgcggttat ccgcaacggg ccaatggcct gacggacaag gccgaaaaga
     8641 tcatcaccga caaccgggtg ctgttccacc gcatggccaa caccctgaat tacctcgaca
     8701 tcaagactgt cgtggtcagt tgcggcacat gctacgacca actggccggc tatgaattcg
     8761 acaagatctt cccgggttgc cgcctgatcg acatccacga atacctgttg gaaaagggat
     8821 tgaagctgga aggagtgcag ggtgtgcgct acatgtatca cgatccctgc cacacaccca
     8881 tgaagctgca ggacccgatg aaaaccgtga agtccctcgt tggcgacacg accatcaaag
     8941 cggatcgctg ctgtggcgaa tcgggaacgc tggccgtttc acgacccgac atctccaccc
     9001 aggtgcgttt tcgcaagcag gaagaactgg cgaagaattc cgccgcggtg cgcgccgacg
     9061 gtttcggcgg cgaggtgaag atactcacct cctgtccgtc gtgcctgcaa gggctgtcgc
     9121 gctacgagga cgataccggc atgcaggccg attacatcgt cgtggaaatg gcgcgtcata
     9181 tgctgggtga ggactggatg cggcaatatg tggctaatgc caacgcgggg ggcatcgagc
     9241 gcgtgctggt ctgatgctgt ggggaggagg cgcttcggct ccctccgtct atttcaggtg
     9301 accatcagcc ccggcatcct ggttcttcgc ggcaacattc gcggtgacca ggcgcgtcgc
     9361 cgggaacgcc agcgcgaaca ggcttccggc attcggacgg ctttccatat gcaggctcgc
     9421 ctgatgccgc atggcgacgt gtttggtgat ggccaggccg agtcccgtgc cgcctgttgc
     9481 tcgcgagcgc ccacggtcga cgcggtagaa ccgttccgtc aggcgcggca ggtgttgcgc
     9541 ggcaatccct ataccggtgt cgcgcaccgc gtagcgcgcc cctccggtgg ctgaacgctc
     9601 ccatgtgacg ctgatggtgc cgccgggcgg ggtatagcgc accgcattgg tcagcaggtt
     9661 gagaaccgcc gaggacagtt cattggcatt gccatgaacg cccagcccct tttcgatgcg
     9721 ctgttcgaac acatgtcgtc ctcccgacag cgcctgtgcc tgggccagcg ccgtggcgat
     9781 gacagcatcc atgtccacgg ggacaccgtc atcgggcggg gaggactcca gtgtcgatag
     9841 cgtcaacagg tcctccacga tggtctgcat acgtccggtc tgttcacgca tcaaatccag
     9901 atagtggtcg cgctgagcgt tcgacaaggc ttctggaggc aggtcccgca acgtttcgag
     9961 gaatcctcct aggacggtca gtggcgtgcg cagctcgtgg gagacgttcg cgacgaaatc
    10021 cttgcgcgtg gtttcgagtt tttccagctg cgtgatatcg cgtgtgacca tgagtagtcg
    10081 gttcagcccg taagggatca actggatctg cagcgttgca tcgcgcccat ccccggcgac
    10141 gcggagaagc aatgggccat cccaatgcgc ttgtttcgca tagcgggcga actcgggggc
    10201 acgaacaatg ttgaagatgc tgtggcccgg gtccgccgac ggatccaagc caagatgacg
    10261 ggcggcttcg tggttgcacc atgtcatgcg cagcgcgtcg tccagtgtca tcacgccgtc
    10321 gggaagcgcc tcggccgcct gcatgatgcc ttgtgcctgc cgctccagca gatccatgtc
    10381 ctggcggttc ttgcgcaacg ctcgatagac cgaggccaat atgtcggccc agtcgccacg
    10441 gatgggtgga ggcggatcgt tgacgttgcc cgcccagtgc gacacccgag caaggcgccg
    10501 gtgttggatt gccatcacgg caagcaagcc cagcgtgaat acggcccacc ctgcctcacg
    10561 gctaagccac gaaccgcaca gccaggcgaa ggcggcccat gcaaccaggc agatggccgg
    10621 acggatcaag gtgtgccacc agccggcgag ggccgcgctg aaaaacggta tccgatcccc
    10681 cgaaccgttt ccacggtgtt gtcctggccc gcgggttcca gtgccttgcg cagtcggcgg
    10741 atatgcacgt ccaccgtgcg ttcctcgacg aacacatgat cgccccatac ctgatccagc
    10801 aattgcgtgc gggaaaacac ccgctcggcg tgcgtcatga agaaatgcag cagccggaat
    10861 tcggttgggc cgagcaccag ggactggcct cccgcgctga cacggtgcga tgccggatcc
    10921 agggacaacg acccggtgtc tatgatgtcg tccgtgtgtt gcggcgcgcg ccgccgcaac
    10981 acggccttga tacgcgcaat cagctccttc ggagagaacg gcttggtgac gtagtcgtcg
    11041 gcgcccgcct ccagcccttc gatcttgtcg cgctcggcgc ttttggccgt gagcatgatg
    11101 agggggacat tgcgggtgcg ctcttgtgcg cgcaggtggc gagccagtgc gacgccctgg
    11161 gtgtcgggca gcatccagtc aagtaaaatc aggtcaggga gctcgtcgcg tatcatggcc
    11221 cgcgcctgcc cgccgtcgcc ggcgcttcgc acctgatagc cggcgaatcc caggttgacc
    11281 gaaatgagtt caagtatcgc tggctcgtct tcgacgacaa gtacagtggt ggtcattggc
    11341 agtgatcgtg gcgccttgaa gaaagaaccg ccatgctacg gtggaaatgt ttcagtattg
    11401 tgaagggtgc gcggcggcgt ggcgcgcccg ttttcccatg tccagccgcg ccagagtacg
    11461 atattactat gcaacatgat tcctcccctg attccccccg cccgtccggc accacgacac
    11521 attttggcta tgccacagtg ggcgaggccg aaaaggccgg caaggtggcc gaagtattcc
    11581 attccgtcgc cagtcgctac gacctcatga acgacctcat gtcggcggga atgcaccgtg
    11641 cctggaaggc cttcacggtg gcgcgcgccg atgtgcgccc cggcatgaag gtgctcgaca
    11701 tcgcgggtgg caccggcgat ctggccaagg cgtttgcgcg ccgggccggc gccaatggcg
    11761 aagtctggct caccgacatc aatgactcca tgttgcgggt cgggcgcgac cgcatggtcg
    11821 accggggcct gttgttgccc acggcggtgt gcgatgcgga acgtctgccg ttcccggacg
    11881 gctatttcga ccgggtcacg gtggccttcg gcctgcgcaa catgacgcac aaggatcgcg
    11941 ccttggcgga aatgcggcgg gtattgaaac ttggtggaaa gctgctggtg ctggagttct
    12001 ccaaggtggc caggcccttg gcaccggtgt acgactggta ctcgttctcg gtgctgccct
    12061 ggctgggacg gaaggtggcg ggcgacgagg gcagttatcg ctatctggcc gaatccatac
    12121 gcatgcatcc cgaccagcaa actctggccg gcatgctcga gtccgctggg ttgtcgcggg
    12181 tgcgtttctt caacctgacc gcgggcgtgg ttgccctgca cgaaggggtg aatctggggc
    12241 ccaacccgca agcctgatca aatcggcgga tgggcggttc cgctgcttcg ccgcttgacg
    12301 ctagaaccga tacgaaacct gcacacccac gagatccgcc gcggtgttca gggtgcccgt
    12361 gaccgtgccc tcggcgggat tggtggcgtt gttgatgtgc gaatggaaga acaggtgttc
    12421 gtagcccacg tccacggtgg cgtgctggtt gaaccggtat tgcacaccca gcgccacact
    12481 gacgcggtca ttgtcgggta gcgacgtcgg ccgaaggtcg ctgccgcgta tgggcgactg
    12541 gtcataggac accccggtgc gcagcttcca gcgcccattg atctggtagt tggcccccag
    12601 cgcgacgcgc catgtgttgc ggaattccag tttgagcgtg tcgtcgccca gcgcaccatt
    12661 gtggattttc agttcaggca ggctggacca gcctgtccag gccagatcgg ccagcacctg
    12721 ccatttgctg ttgatgtcgt ggaccacgct cagggtggca gtgtcgggga gggtggcgga
    12781 cgcgctgttg tcgaccgcgc ccggtatggc tgagctgaac gcgccgggga tgccggcgag
    12841 cgaggtggtt ccttccaggg gcacatgcac ccgcgaacgg tacgccagac cgatgcgagt
    12901 ctgatccgtg gcccggtaca gcaggcccat gttccagccc cagccactgc cgtgcaacct
    12961 ggctcgggcg ttcatgtcgg gtgatgccat caactgtgaa gccagcgctt ggcggacaga
    13021 cccgggcagg gtggatggaa tttgcgggat caagcccgcc gtgggcacgt tgcggcgata
    13081 gtcggcatcg gcgtacagcc agtcgagccc gacgcccagg gataacctgg aactggcttt
    13141 gtaggccacc gatggattga tgttgacaga ttgcaggtcg aagcgggtgg attgatagcg
    13201 gcccacccag tcactgtccc agtcggttga cagcccgtat ggggcgccaa cccccaggcc
    13261 gacgaaccag cttggatcga gttgccagga ggcgtagagg ttgggaagaa aagatgtacc
    13321 gccggcatcg cccccctggt tcgtgcccag gggtaggcca ccagggccgg tgctgccatt
    13381 gtccgagaac ttgaacgtgg tggtgatggc cgtcatgccc gccgacaggt tgaccccagg
    13441 caggcgagtc atgcccgcgg gattgtagaa aatggtgctg gcattgtcag cgatagcggc
    13501 cgaccccgcg taggagttgc ccaggccgct ggcgttttgt tcgatcaact ggaaagcgga
    13561 cgcgttggcg cgtgagcatg tcaggcccag caacagtgcc gacgccatat aagggagaag
    13621 tttccgggca tatcggctga acggcttagt gttgtaggat gtgagcatga tttggcgaag
    13681 taaattatcg ttacgtttga tccctggagc ggggcacctg catgtgtgga gaaaatactg
    13741 aattgatacg gatttttcca aaaacagctt gcaaattgta atgtgttttg taaaaagatc
    13801 attgtttgtc tttaatttgc cagcgttttt gttgtctgaa tgcactatta ggacaaatgc
    13861 ctgacgtgcg ggtttttatt cttgtgcaat atatggttgg cgaatatttc tgagccaatc
    13921 tatactgagt caatgtgcgg aattgtcacc tgctattaca gcaaacggcc tgcccctgga
    13981 gcacagacgc cgggcttggt catggttgga tcttctgctg aggaatcaag aggtatgtca
    14041 tgctcgatac catgagcgat gttagcgatt cgctgctttt cgcctatttc gggacccgta
    14101 gtccgttctg gcaactgtca gccgacagca acgccctgca attggcgggg acacaaggta
    14161 caccgaacct cgcggtcgcg ctcgcccccg acaaggcgcg tgaaatccgg gcgttcacgg
    14221 gcgttacgtc gcacgccgaa atcgaaatcg agctattcgg cgaacctcag aaactcagtc
    14281 tggtcggcaa gaagaacggt gccaactcgt ggtcggggac ggcgtccgac tacatggaca
    14341 cgcccgcagt ggccaaggat ctggcgcacg ggctcgagtt cgccgaacag gtcatttccg
    14401 aggtcaattc gctggtcgtc atcatggacc acaacctcaa catcctgcgc ttcaaccgct
    14461 tgtgcgaaga agtcaccggc ctgcgtgaaa aagacgtcat cggcaaaccc gcccacgcct
    14521 tcatgtccat gagcgagcgt gatgcctcca aggccaacat cgctgacttt ttcaagaatt
    14581 ccgcagccta cgaagtcgaa cgccatatca acacgctcaa tggtccgcgc accatcttgt
    14641 ggcgcaacaa attcgtgcaa agcggcagcg gtcccgatga gcgctacctg gtgtgctccg
    14701 gcaccgatgt gaccgaagag cgcaaggcgc aggccaagct catggaactc gccacggtcg
    14761 acaccctcac ggggttgccg aaccgtcatg ccatttacga gcaaatcaac cgatcgctca
    14821 ataacggtga acgtttcggt atcctgttcc ttgatctcga caacttcaag caggtcaatg
    14881 accattacgg tcacttggtg ggcgacagcc tcattcaggc agtggcgctg agcatcgttt
    14941 cgtgcatgcg ggaaggcgac agcgtggcgc gcctgggcgg tgacgaattc ctggtgttgg
    15001 tcaagaacgc atccttcgag ctcatggaaa acgtggcgca gcgtatcacc gatcggctga
    15061 aggcgcccat cagcctgggg ctggtcgagg tctataccgg gtgttccatc ggtatcgcac
    15121 tgtgtccgga acacggcgac tcgatcgatc acctcgtccg ctgcgccgac acggccatgt
    15181 atgtcgccaa agattccggc aagcgcacgt accgtgtgtt ttcgccagac atggacagcc
    15241 gggtggcgga gtacatctgg ctcgacagca atctgcgcaa ggccctcgac gccgaggatc
    15301 aactgcagtt gtattaccag cccaagctct gtctcgagac cggcgaggtt cgttcggttg
    15361 aagccctgtt gcgctgggac tcccccgaac gtggccgcat atcacccatg gacttcgttc
    15421 cgtacgccga agagtccggc ctgatcatgc cgctcggccg ctgggtcatg cgcaccgcgg
    15481 ccaagcaggc ggccgcatgg aaggcgcgtg ggctcgacat gcgcatcgcg gtgaacctgt
    15541 cggcgcggca gctcagcagt tccaccttga tccaggattt ctccgaagcc ctgcgccagt
    15601 ccgaccagac gcgctcgctg ctcgatatcg aactcaccga gagctgcctg attcacgacg
    15661 aggccgccgc gctcaggctc atccagcagt tccgcgaact cggcgccgag gtgcacctgg
    15721 atgatttcgg cactggatat tcgtccctgt cgcaaatcgc ccggcttcct ctcgatgcca
    15781 tcaagctcga tcgcagtttc gtgaagtctg tcgacgaaaa cgccacgtcc caggcgctgg
    15841 ttcgatccat ggtggtcgtg gcgcaagaat tgaagttcaa ggtcatcgcc gagggcgtcg
    15901 aaacctccgg cgaggcggag ttcctcaagg atgtgggtgt cgagtacacg cagggctacc
    15961 tgtacggtcg cccgatgtcg gtcaaggaat tcgaagactg gctcgataac aagggcaagc
    16021 ggcttcgcct agtggcctga cggtgttcga tgcgcggcct cgggccgcgc atcggtgtat
    16081 gcggggaatg gtgcgggtct tgtctcaggc gacgttgcgc gcctgtttcg cgtgccggtt
    16141 ctgcagcatg accaggcgtt ccatatattg caggtctttt tcttccaggg tgaacgcggc
    16201 ctgggcccaa tcctcggtga tttccatgag ttccgagcga tcgatagaca gcacccgctg
    16261 gcgtgctcgc agcatggccc ggatgccgtt catcttgggc cgaatgacat caatgagcgt
    16321 gcgggtcgcc atgaacgcat cgcccttttc gaacacctgg tccaccagcc ttttttcctg
    16381 ataccactcc gcggtgtgtg aaacgccggt gctgatcagc tcttcggcca gcagcatgtg
    16441 ggatttgcgc gccaccaatg aataggcgcc catgccgggg aacaggttga aggcgatctc
    16501 tggaaacccc attttcacat cgcgctgggc caggaaatag tggtgcgcca aggctgcttc
    16561 gaaaccgccg ccgagcgccg aaccctcgac catggcgatg gtgatcgcgc cggtgccgaa
    16621 gccaaccatg gcatcatgga tggcatcgat acaagcgcgc gcgtacgccc gcaattgctc
    16681 gtgctcaccg gcgcgaatgt gtgcggcgaa gagttcaaga tcgccgccga cgttgaatat
    16741 ttgcggcacc acggatccgg tcacccagaa atcgatcggt agccctgaat cctgtgctgc
    16801 ccgcttcagg cgcgagatgt cgtgtagcag cgcggtgttg aagcaggggc gtggctgagc
    16861 gttgagcatc atccacagca cattgcgccc ttcttcgtaa taaggggtga tttgcgtgag
    16921 gttgtccgct tcggaaaaaa gccgacacga tggatggtct tccagtctca tcgtcgggat
    16981 aggtgccttg aacgggtgcc agtggcccgc gtcattggtg atggcgccag gtgttcctgg
    17041 gagaaagtga cgcgcctcat tccgccggga cggagcgtgc cgtcaggctc gtgacgggaa
    17101 cgagacgccg cgtgtcgcgg gtggctgaag agaagggggg cggaaaaggc gccaactttg
    17161 cgaatttctt gagcctggag ggcgatttcg cgataagcac ggcatcgagg ggggaggtca
    17221 tgacttaatc tgcaaggagg cgtaaacggg gtggccgtgt gctgtcaggt ttccgagaat
    17281 ggcatgtcga acatgttcgt tactgttcgt atactaacag agaaaatgtc tggcacagaa
    17341 gggatttgcc cgtggttttg cgcctttttc cttatgtggg tcccggtcat tatgcctctt
    17401 gtcgcaaagt cttggtttgc cggcatcgcc gcctcacgaa taattaatgg ttgcaccttc
    17461 tattcagctt tggttcaggt taatattcag tttgccgtca gtttctgttt cgtggcatgg
    17521 tgtccaatat gccgttgccg cgaaacgaac ttcaaacgtt ttttcatcct gaccaccgga
    17581 gcgttccatg tcacggttct tttccaagca tctcgtcacc gccctactgc tcgtttcgtc
    17641 cctgggcatg ctgacggcgt cattcaacgc cgaggccagg cgcatgggcg ggggcggaag
    17701 cttcggtcga caatcgctca atgtcaccaa gcagcgccag gccatcacgc ctccggcggc
    17761 caagtcgccc gccgccgcag cacagaattc cacggccggt gcccgcggcg gggcgtcgcg
    17821 ctggcttggc ccgttggcgg gtattgccgc cggcctgggc attgcggcgc tgttgtcgca
    17881 cctgggattg tcaggggcgt ttctcgaaac catctccagc atggtgctga tcggcctggt
    17941 gctcgccgcg gtcatattcc tggtgcgtcg gctgttggcg ggagcacgcg cacccgccag
    18001 cgcggctgcc ggcgacgcgg cgcccggcgg cgcgttgcgc agcgcctgga acagcggcgg
    18061 ctcgccacaa cgacccgcgg gtgcagcatc gtcgtcgcac gcgcccgccg caatgtcgcc
    18121 ttcgttcgcg gcgtcatcgg tgcctgtcga tggtgattcg aactgggtca ttccacatga
    18181 tttcgactca gtggctttcc tggcccaggc caagaagcag ttcgccgcca tccagaccct
    18241 ctgggacagc ggcgaagtca accagctggc aggctacctc accgatgatc tgatggctga
    18301 aatcgcgccc caagtggccg ccaaccaagg cgccaagccc actgaaatcg ttttgctcaa
    18361 cgccgaattg ctgggtgtgg aatcagtgga agggggccat ctggccagcg tccggtattc
    18421 aggcatgctg cgcgaagacg ggcacaccga ggcattccgg ttccaggagg tctggaatct
    18481 gtacaagagc gccgccgatg gctggttgct ggccggtatt cagcaaattt ccgcctgatc
    18541 ctttcaggcc ggcgctcccg gccagccagt gtctcttcca tggggcggct ctacagccgc
    18601 cccaaaaccg ttaaacttga acattcccaa ccattctccc tgacaggcgc ggggggtcgc
    18661 ggctgtctga accatgtttc ctattccgtc cttcctggcg cccgccgccg tgggcgcccg
    18721 cgtattgaac gccttgctgg cgcgtgaaga ctgggcccgg gaacgcttgg ctcgccacgc
    18781 gggcaagacg gtgcgattcg ttgtcggcca catgacggtc ggtctggtca tcgccagtga
    18841 tggcagtgtg tcggccggca acccgtccgt ggtgcccgat gtcacgctca ccatccctcc
    18901 cgaacgtctg tcggaacttc cccaggccct gcgctccagc gattccgagc gcatcgctgg
    18961 tctcatgcac atccagggcg acgcggccct ggcgcatggg gtggcgggtc tggcgcacga
    19021 tttgcgctgg gacgtcgaaa acgacctgtc gcgactggtg ggcgatgtcg ccgcgcgacg
    19081 gctggtatcg gcattccggg gtctggccgt gggcgcccgc cgcggcacgc atcgactggc
    19141 cgccaacctg ggcgagtacc ttgccgagga aagcgctttc ctgtcggggc gcgatgcgta
    19201 tgtcgactgg gccgccgggg tacgggcggc cggtgcgcgg ctcgacgcgc tcgagaaccg
    19261 ggtcgcccgc ctttctcgtc cgggacctca tcccggacac aagggcggtc gctgaccatg
    19321 cctacgctat tccgcttgat tcgcatcatt ttcgtggcat tgcgctaccg gctcgacgag
    19381 ctggtgctct cgagcatcaa gcatccgctg gcttacgggc tgttgcggat cgtgcgtttt
    19441 ggccgaacgc cccgcacccc ccgtggccag cgtctgcggc tggcgctgga gtcccttggg
    19501 ccgattttcg tgaagttcgg tcaggtgctg tccacgcgcc gcgacctgat ccccctggat
    19561 gtggccaggg agctcgcgtt gttgcaggat cgtgtaccgc cgtttccatc tgatcaggcg
    19621 gctcaatgcg tacaggacgc gctgggtgaa tcgccgcata cgctcttcaa gtcattcgat
    19681 gccgatccgg tcgcgtcggc atccatcgcg caagtgcatt tcgcggtgct gcacgacggc
    19741 cgcgaggtcg cggtcaaggt gctgcgcccc ggcatgctga gcatcataga aaaagatttg
    19801 cgtctgctgc gcgcgctgtc ggggctggtc gaacgcctgg tgccggacgg ccggcgcctg
    19861 aagccccggg aggtggtggc ggaattcgac aaatacctgc acgacgagct cgatctcatt
    19921 cgcgaggcgt ccaattgcag ccaactgcgg cgcaacttcg ggcctgacac cgagcgcggg
    19981 aacctgctga tggtgccgga agtggtgtgg gagtattgcg ccaccacagt cttcaccatg
    20041 gaacgcatgc gtggtgtgcc catcagccgc gtcgagcagc tgcgcgaact cggcgtcgac
    20101 atcaagaacc tcgcgcgcac cggcgtcgag atattcttca cacaggtgtt caccgacggt
    20161 ttctttcatg ccgacatgca tcccgggaac atctatgtct cggatacgcc ggaaacgctg
    20221 ggacgctata tcgcgctcga tttcggcatc atgggttcgt tgtccgagtt tgacaaaaac
    20281 tatctggcac agaatttcct ggcgtttttc cggcgtgatt accggcgcgt ggcgcaactg
    20341 cacctcgaat ctggatgggt tcccgaaaac acgcgcgaag aagagctcga gggggcggtg
    20401 cgcgccgtgt gcgagcccta tttcgaccga ccgttgtctg aaatctcgct ggggcaggtt
    20461 ctgttgcgcc tgttccagac atcacgtcgg ttcaatgtgg aaatccagcc acaacttgtg
    20521 ttgcttcaaa aaacgctgtt gaatgtggaa ggcatggggc gcgacctgga tccgagcctg
    20581 gatctctggg aaaccgccaa gccgtatctg gaaaagtgga tgaggcagcg tgtagggctc
    20641 ggcgcgctgc gtgtgcggtt ggcccaggag gccgggcagt gggcgcaaat ccttccgcag
    20701 attccgcggc tggtccacgc caacctggtc cggcccgatt cccaggcgca cctgcatgcc
    20761 gaagtgcgtc gcatgcgtca ggagcaacga cagaccaatc gcctgctgtc ggcgctggtc
    20821 gcggtgctgg cggtcgctat cggggtggca atgtgggcgc tgacgcgctc ttagcggcag
    20881 gcttgggggg ctgaatcgcc cccgcgcttg ccgatacgcc gggtgctggc actgcccgga
    20941 aaccagacaa ccggcgtgtg actcgagcgg agagaaaccc gcctgcgccg aacttcaaga
    21001 gggatcctat gctttatccg gaattgttca aatcgatgga ggcggtacgc tggaacatgg
    21061 catccgacat ccca
//
LOCUS       DASTNK010000055        12559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_594986, whole genome shotgun sequence.
ACCESSION   DASTNK010000055 DASTNK010000000
VERSION     DASTNK010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12559
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594986"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VFR20_04155"
     CDS             complement(<1..396)
                     /locus_tag="VFR20_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU0229740.1"
                     /translation="MDAFDAPLPVRDGVAPSRVFLPRGPWQTLLGFLIERFPYVGEDV
                     LRHRLDAGQIVDEAGVAQAAGDTYRPLRWLWYYREVPDESPVPFEVRVLYRDARLVVV
                     DKPHFLASVPSGRYLRETALVRLRRDLDLP"
     gene            complement(418..1455)
                     /gene="tdh"
                     /locus_tag="VFR20_04160"
     CDS             complement(418..1455)
                     /gene="tdh"
                     /locus_tag="VFR20_04160"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEU0229741.1"
                     /translation="MQALVKLEPAEGLVLSSVERPSVGHNDVLIKIRKTAICGTDIHI
                     WKWDEWAAKTIPVPMQVGHEYVGEIVEIGQEVSGFKIGDRVSGEGHITCGYCRNCRAG
                     RRHLCRNTSGVGVNRPGAFAEYLAIPAFNAFRIPDDIPDELAAIFDPFGNATHTALAF
                     DLVGEDVLITGAGPIGAMAAAIARHVGARNVVITDVNDYRLDLARKMGATRAVNVSRE
                     KLADVALELGMNEGFDVGLEMSGAPAAFTSMLEHMNHGGKIAMLGIPPAGFGVDWSKV
                     IFKGLQIKGIYGREMFETWYKMVAMLQSGLDISPIITHRFPVSEYKTGFDAMLSGQSG
                     KVVLDWASDRA"
     gene            complement(1546..2772)
                     /locus_tag="VFR20_04165"
     CDS             complement(1546..2772)
                     /locus_tag="VFR20_04165"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEU0229742.1"
                     /translation="MPIAPHAHNPGAFLSHVRGQLHTLRDEGFYKSERVMAGAQAASV
                     LLEDGARVLNFCANNYLGLANDARLIAAARDGLGEAGFGMASVRFICGTQTGHKALEH
                     ELAGFLRMGDAILYSSCFDANGGLFEALLGEEDAIISDALNHASIIDGVRLCKARRYR
                     YANNDMADLEAQLRQADEDGVRFKLIATDGVFSMDGIIANLKGICDLADRYGAMVMVD
                     DSHAVGFIGGHGRGTPEYCGVEGRVHILTGTLGKALGGASGGYVAAHEAIVELLRQRS
                     RPYLFSNSLAPSIVAASRRVLALLGGDEGGALRQRVHANGRRFREAMAGLGFSLVPGE
                     HPIIPVMLGDARLAGQMADLLLREGVYVIGFSYPVVPKGKARIRTQMSAAHTQDQVDE
                     AVAAFARVGRRLGVIA"
     gene            complement(2948..3424)
                     /gene="pgaD"
                     /locus_tag="VFR20_04170"
     CDS             complement(2948..3424)
                     /gene="pgaD"
                     /locus_tag="VFR20_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HEU0229743.1"
                     /translation="MIIKTPRSRVSALIDFALTLLAWIAFGYLFIDGIVSIVEGNTPG
                     LAVPFMSRLLPELRTLLVYVVVAAIIALVLFAWAKYNAARFGGLDRRKTPPALSSEAL
                     ADSFEISADQLATLRESRRATIHHTPDGLISGIEASDVRGSASVVNLVQRSRTVGD"
     gene            complement(3421..4758)
                     /gene="pgaC"
                     /locus_tag="VFR20_04175"
     CDS             complement(3421..4758)
                     /gene="pgaC"
                     /locus_tag="VFR20_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HEU0229744.1"
                     /translation="MIDRLFAFLILCAVLGVPFGVALLWTGDKILDFVFFYPLFMSGL
                     WMFGGLYYWLHWERHWKWGNGVQPPELRGNPQVAVLVPCFNEVEHGRDTILAALDQHY
                     PNVEVIAINDGSSDGTGAMLDELAASHERLRVIHLAQNQGKAMALRMGALAARSEYLV
                     CIDGDALLDPDAVSYLIAPLLDNPRVGAVTGNPRIRTRSTLVGRVQVGEFSSIVGLIK
                     RTQRVYGQVFTVSGVVAAFRRSALDRVGYWSLDMVTEDIDISWKLQRDHWSIFYEPRA
                     LCWILMPETLRGLWKQRLRWAQGGAEVFLKNIRNIWGWRHRRLWPMLLEYCLSAIWAF
                     AFILSIILWILGHYMNLPSHIHIETLVPPAFTGMVLAMVCLMQFVLSICIDHRYEPGL
                     PRALYWVIWYPLAYWLVSLLTTLFSFPKVMLKHRRRRARWTSPDRGIKAPTRS"
     gene            complement(4755..6776)
                     /gene="pgaB"
                     /locus_tag="VFR20_04180"
     CDS             complement(4755..6776)
                     /gene="pgaB"
                     /locus_tag="VFR20_04180"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HEU0229745.1"
                     /translation="MSSLLSRLRFVFLACVVLLLAACARDIPKFVPPAERPVANVDKP
                     WPVNHLLTLAYHDIEDHGRNQIFVAVRTDLFVSQMHWLHDNGYQPVSVDQILAAHQGG
                     KPLPPKAVLLSFDDGFRSFYTRVYPILKAFEWPAVWAPVGDWVSTPPGHTVNFGGVIE
                     PRKRFANWEEVREMSRSGLVEIASHTYAQHEGIPANPEGNLQPAETTHRYDAATHTYE
                     SDAAYRKRIRDDARRITEKLRQVTGKPPRVWVWPYGAASGEAISIIGKQGYKMALTLD
                     DGLGTVNRLMSEPRVLVSDSPGMADFAETVLSTEARRSMRVVQVDLDYVYDPNPAQMD
                     KNLGVLVQRIADMGVSTVFLQAFADPKGDGLVKSVYFPNHWLPVRANLFNRAVWQLQT
                     RAHVKVYAWMPVLSFDLDPSIARVTRWNPKHPDAPPTVDPKQYKRLSPFDPRARTAII
                     GLYEDMARSAPIDGVLFHDDAVLSDFEDAGPQALAAYRKAGLPGSIQALRADPATMRR
                     WTRFKSQYLIDFTNTLTRHVRAVRGPQVKTARNIFVEPILNPSSEEWYAQNLGDFLKA
                     YDWTVPMAMPLMEKVPASQAGAWLDHLVATVKKYPGALDRTVFELQTRDWNKPGEPAI
                     SSKELAGWMQRLQLDGVRSFGYYPDDFIRNEPVLKTIRPAISNAWYPYK"
     gene            complement(6791..9244)
                     /gene="pgaA"
                     /locus_tag="VFR20_04185"
     CDS             complement(6791..9244)
                     /gene="pgaA"
                     /locus_tag="VFR20_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HEU0229746.1"
                     /translation="MSLHSSRVTIAICAVFAALPTLSWAASPAGYDKLIREARSGDYQ
                     PALSMLSQRLHSAPSDRRVLYDYIVISDWAGHSRQVAAAYEAAGSPNNLPAEPLLAVA
                     RAYRNDKQWNKAIALYREGERRFPSQQGFALGRIMTLADARRIDEAVGLGQKLVAREP
                     RNADSRLALAYAEHIGGEPYAELEQATKAYGLAPGKAYVVRAYILALQRARLSQAALR
                     EAQAHPGVMDAAQVRGLQADVAAQLTRVAAASSRGEANRFKLADRALAMYDKLIPEWK
                     ALGPSAHADVVRAEADRILALHARMRMKDIVTSYEAMLAQGTTVPDYVLGDVAAAYLY
                     LRQPEKAAALFHRALASPAASHDPDTRFADQAGLFYALTESGNFKQANTELNAAVSEQ
                     PVWLYVPGDRSRQPNNLRLDADLTRAMGYLYEGHASKAQARLDQMVNEAPNNTRLRTA
                     RSQVYRARELPRHAEHDLKIAETEGPRSVDVETNQAETALDLQEWRQAKILRDDVVAR
                     RPEDMSVQRLAREWDVHNMSELRVTASRGVSTNSPVMGSHDWNIDSVLYSPPINENWR
                     GFAGMGYSTGEFEEGSGNYRWMRAGAEWRSRDVTAQAEVSGNSFGYGIRTGAALSASV
                     DLNDHWQVGGGAAVMSRNTPLRALKHDITSNSLNAYVRWRGDERREWTFSFTPSHFTD
                     GNNRMELDLSGRQRLYTDPNLKIDALLDLSASHNTLDNTPYFNPKSDFTALPSLRFTQ
                     VLHRRYETVWEQQFLVGAGAYSQQDHGTAGIVTVGYGQRFRYNDVLDVGFMLSGTSRP
                     YDGQRERDYSIVVDMTYRF"
     gene            complement(9639..9866)
                     /locus_tag="VFR20_04190"
     CDS             complement(9639..9866)
                     /locus_tag="VFR20_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6356 family protein"
                     /protein_id="HEU0229747.1"
                     /translation="MSKFTQMFTTHPASVGESYFQHMAASFSFGVPMAGAALAAIVHG
                     IFPFLFVKTGSGIVSRLYDRMVTHRSRKVSE"
     gene            complement(10078..11637)
                     /gene="rmuC"
                     /locus_tag="VFR20_04195"
     CDS             complement(10078..11637)
                     /gene="rmuC"
                     /locus_tag="VFR20_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU0229748.1"
                     /translation="MTELNPGLVPGLAGWLLALTGLLVGVLVTLVCFRRVLARIRTEL
                     TLSQASNTQRGETITALRADLDQSRTKSIALDAEKGHAERQLARAESDSSSLRERLAE
                     AKSALADKEAALRATGDRLNEVNRELAEMSTTYKQKVAHFEELKASFDQSRLQLKTEF
                     QNLANQILEEKGKTLAANSQASLEVLLKPFREQIEGFQRRVNQVHDESVRGNVSLGVE
                     IKRVLEIGMKMSTEANTLATALKGDKKTTGNWGEVQLELALQQAGLVRGDHYESQPRF
                     KDVDGQPRQPDFVVMLPDQKHLVIDSKVSLVDYDRAIAAGTQAEFDAALDAHVKAIKN
                     HIDDLAGKDYSNLIGVHSPSFVLMFMPIEPAYIEALKHNKSLFNYGYEKNVIMVSHTT
                     LMPILKTVANLWMVARSNEQAHEISAKAAEIYNQVVLVAERLKRLGDTLGTASRHYND
                     TVTAVAGQQGLYGKTARFSELSAKANKTMPSLDPVHRDFETHKLELVATPVTPAPADP
                     ADVSRRRILPQ"
     gene            complement(12148..>12559)
                     /locus_tag="VFR20_04200"
     CDS             complement(12148..>12559)
                     /locus_tag="VFR20_04200"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009456821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEU0229749.1"
                     /translation="PVLAHVGLMPQYKNTMGGFKAQGMTDESAERILQDALAHEEAGA
                     FAVVLEGIAEGLARRMTESLRIPTIGIGASPDCDGQILVTEDILGLSGSKIPKFAKQF
                     ADVGAVIRAAVEQYAADVRAGTFPGLDQCFGVKK"
BASE COUNT         2540 a         3929 c         3718 g         2372 t
ORIGIN      
        1 gggaagatcc agatcacgac ggagacgcac cagcgccgtt tccctcagat aacggccgct
       61 gggcacactg gccaggaagt ggggcttgtc cacgacgacc aggcgggcat cccgatacaa
      121 cacgcgaacc tcgaacggca cgggtgattc gtccggcacc tcgcggtaat accaaagcca
      181 gcgcagcggc cggtacgtat cgccggccgc ctgggccaca cccgcctcat cgacgatctg
      241 gcccgcgtcc agcctgtgcc gcagaacatc ctccccgaca tagggaaagc gctcgatcag
      301 gaaccccagc aatgtttgcc agggaccgcg cggcagaaac acgcggctgg gcgcgacacc
      361 gtcacgcacc ggcaagggcg catcaaaggc atccaaagcg ttatggcccg ataacgtcta
      421 ggcgcggtcg gatgcccaat ccagcaccac cttgccgctt tggccggaca gcatcgcatc
      481 gaatcccgtc ttgtattcgc tcaccgggaa gcggtgcgtg atgatgggcg aaatgtccag
      541 cccgctctgc aacatggcga ccatcttgta ccaggtctcg aacatttcac ggccgtagat
      601 gcctttgatc tgcagcccct tgaagatgac cttgctccag tccacgccaa acccggcggg
      661 cggaatcccc agcatggcga tcttgccgcc atgattcatg tgctcgagca tgctggtgaa
      721 cgcggccggc gcgcccgaca tttccagccc cacatcgaag ccttcgttca tgcccagttc
      781 gagcgccaca tcggccagct tttcgcgcga gacattcacc gcgcgcgtcg cccccatttt
      841 gcgggccagg tcgagccggt aatcattcac atcggtgatc accacgttgc gcgcccccac
      901 gtgacgggca atggccgccg ccatcgcgcc gatggggccc gcgccggtga tgagcacgtc
      961 ctcgcccacc aggtcgaagg ccagcgcggt gtgcgtggca ttgccgaacg gatcgaagat
     1021 cgcggccaat tcgtcgggga tgtcatcagg aatgcgaaat gcgttgaacg ccggaatcgc
     1081 cagatattcc gcaaaggcgc caggccggtt cacccccacc cccgacgtgt tgcggcatag
     1141 gtggcgccgc cccgcgcggc agtttcggca atacccacac gtgatgtgcc cttcgccgga
     1201 gacgcgatca ccgatcttga acccactgac ctcctggccg atttcgacga tctcgcccac
     1261 gtactcgtgc ccgacctgca tgggaaccgg aatggtttta gcggcccatt catcccactt
     1321 ccagatgtgg atatccgtgc cgcagatggc ggtcttgcga atcttgatga gaacgtcatt
     1381 gtggcccacc gagggtcgtt ccacggacga caaaaccagc ccctcggctg gttcgagctt
     1441 taccaatgcc tgcatcgtat gctccagaaa ttcatattcg tgcggaaggc tcaagagatc
     1501 gcccacatgc tgttcgggtg atctgaacgc gccaaccgac ccgcgctagg cgatcacgcc
     1561 caggcgacgg ccaacccgtg caaacgccgc gaccgcctcg tccacctggt cttgcgtgtg
     1621 ggccgcgctc atttgcgtgc ggatgcgcgc cttgcccttg ggcaccaccg ggtaggaaaa
     1681 cccgatcaca tacacccctt cccgcaacag cagatccgcc atctgtcccg ccagccgcgc
     1741 gtcgcccagc atgacgggaa tgatgggatg ttcgcctggc accagactga aacccaggcc
     1801 agccatcgcc tcgcggaacc gccggccatt ggcatgcacc cgttggcgca gcgcgcctcc
     1861 ctcatcacca cccagcaacg ccagcacccg gcgcgaggcg gccacgatgc ttggcgccaa
     1921 ggaattcgag aacagatacg gacgcgaacg ctggcgcaac agctcgacga tggcctcatg
     1981 cgccgccaca tatccccccg acgcgccgcc cagggccttg ccgagcgtac ccgtcaggat
     2041 gtggacacgc ccttcgacac cacagtactc cggtgtgccc cggccatgcc cgccgatgaa
     2101 acccacagcg tgagagtcgt cgaccatgac catggcgccg taccgatccg ccaagtcgca
     2161 gatgcctttc agattggcga tgatgccatc catcgagaac acgccatcgg tggcgatgag
     2221 cttgaagcgc acgccatcct catcggcctg ccgcagttgt gcttccagat cggccatgtc
     2281 gttgttggcg tagcgatagc gccgagcttt gcaaagacgc acgccgtcga tgatgctggc
     2341 gtggttcaac gcatcactga tgattgcgtc ttcctcgccc agcaacgctt cgaacagccc
     2401 cccattggcg tcgaaacaac tggaatacag aatggcgtcg cccatgcgca ggaagcccgc
     2461 aagttcatgt tccaacgcct tgtgtcccgt ctgcgtaccg cagatgaaac gcaccgacgc
     2521 catgccgaaa cccgcttcgc ccaggccgtc gcgggcggcg gcgatgagcc gcgcatcgtt
     2581 ggccagccca agataattgt tggcgcagaa attcagcacc cgggcgccgt cctcgagcag
     2641 cacactggcc gcctgtgcac ccgccatgac gcgctcggat ttatagaaac cctcgtcgcg
     2701 gagcgtgtgg agctggcccc gcacgtggga aagaaacgca cccggattgt gggcatgggg
     2761 ggcaatgggc atgacagcac cttcgaccaa aggaagcatt aagcgagaaa cagactggtg
     2821 ccgggaaaac accggccctt tcttcctgga atcttaacgc gcctgcatgc ggatgcgcga
     2881 attggagcca aaagaccagg ggaatgcctc acccgccgcc gattcgatcc tgaagcctgg
     2941 tagcgcctca atcgccgacg gtgcgactgc gttgcaccag attcaccacg ctcgcggagc
     3001 cgcgcacatc gctcgcctcg atgccgctta tgagaccatc cggcgtgtga tgaatagtcg
     3061 cgcgccggct ctcgcgcagc gttgcgagct gatccgcgga aatctcgaaa ctgtccgcca
     3121 gcgcctcgct cgacaacgcg ggcggcgttt tgcggcggtc gagcccaccg aagcgagcgg
     3181 cgttgtattt ggcccaggca aacaacacca gggctatgat ggcggccacc accacgtaaa
     3241 ccagcagcgt gcgcagctcg ggcagcaggc gcgacatgaa cggcaccgcc agacccggcg
     3301 tgttgccctc gacgatggac acaatgccat cgatgaacag ataaccgaag gctatccagg
     3361 ccagcaaggt cagggcaaaa tcgatcaggg cggataccct ggagcgtggg gttttgatga
     3421 tcatgatcga gtgggcgcct tgatgccgcg atcggggctg gtccagcgcg cccggcggcg
     3481 acggtgcttg agcatcacct tggggaaact gaacaaggtg gtcaataggc tgaccagcca
     3541 atatgccaat ggataccaga tcacccagta cagggcccgg ggcaggccgg gttcgtagcg
     3601 atggtctatg cagatgctga gcacgaactg catcagacac accatcgcca gcaccatgcc
     3661 ggtgaacgcc gggggcacca gggtctcgat gtggatgtgg gatggcagat tcatatagtg
     3721 gccgaggatc cacagaatga tggacaggat gaacgcgaac gcccagatgg ccgagaggca
     3781 gtattccagc agcatgggcc acaagcgtcg atgccgccat ccccagatgt tgcggatatt
     3841 tttcaggaag acttccgccc cgccctgcgc ccagcgcagg cgctgcttcc acagcccacg
     3901 cagggtttcc ggcatgagta tccagcacaa ggcgcgcggt tcgtagaaaa tggaccagtg
     3961 gtcgcgctgc agcttccaac tgatgtcgat gtcctcggtg accatgtcca ggctccaata
     4021 gcccacgcgg tccagcgcgc tgcgccggaa ggcggccaca acgccagaaa ccgtgaacac
     4081 ctggccatag acgcgctggg tacgctttat gagccccacg atggacgaga actcgcccac
     4141 ctgcacacgg ccgaccagcg tggaacgcgt gcggatacgc ggattgccag tgacagcgcc
     4201 gacacgcggg ttgtcaagca atggcgcaat caagtaagac accgcatccg gatccagcaa
     4261 cgcatcaccg tctatgcaga ccaggtactc gctgcgcgcg gccagggctc ccatgcgcag
     4321 cgccatcgcc ttgccctgat tctgggccag atggatgacc cgcaggcgtt cgtgcgaagc
     4381 ggcgagttcg tcaagcatgg cgccagtgcc gtcgctcgag ccgtcattga tggcgatcac
     4441 ttcgacattg ggataatgct ggtcgagcgc ggccagtatg gtgtcccggc catgctcgac
     4501 ctcattgaaa cacggcacca ggaccgcgac ctgcggattc cccctgagct cgggcggttg
     4561 tacaccgttg ccccatttcc agtggcgctc ccagtgcagc cagtaataga ggccgccgaa
     4621 catccacaga cccgacatga acaacgggta aaagaacacg aagtccagga tcttgtcacc
     4681 ggtccagagc agagccacgc cgaatggcac gcccaggacc gcgcacagta tcagaaaggc
     4741 gaatagtcga tctatcattt gtatgggtac cacgcattcg agatggcggg ccggatagtc
     4801 ttcaatactg gctcattccg gatgaaatcg tcggggtagt agccaaagct gcgcacgccg
     4861 tcgagttgta gccgctgcat ccaaccggca agttccttgc tgctgatcgc gggttcgccc
     4921 ggcttgttcc agtcgcgggt ttgcaactcg aataccgtgc gatcgagcgc gccgggatat
     4981 ttcttcaccg ttgccaccag atggtccagc cacgcgccag cttggctggc cggcactttt
     5041 tccatcagcg gcatcgccat gggaaccgtc cagtcgtagg ccttcaggaa gtcgcccaga
     5101 ttctgggcat accattcctc gctggagggg ttcagaatgg gttcgacgaa gatattgcgg
     5161 gcggtcttga cctgaggccc ccgaacggca cgcacgtgcc gggtcagggt attggtgaaa
     5221 tcgatcaggt actggctctt gaaacgcgtc cagcgccgca tggtggccgg gtcggcgcgc
     5281 aatgcctgga tcgacccggg caaccctgcc ttgcggtacg ccgccaacgc ctgcggtccg
     5341 gcgtcttcga aatcgctcag caccgcatcg tcatggaaca ggacaccatc gatgggtgcg
     5401 ctacgcgcca tgtcctcata caagccgatg atggccgtgc gggccctggg gtcgaatggc
     5461 gacaggcgct tgtattgctt gggatcgacc gtgggggggg catcggggtg cttgggattc
     5521 caacgtgtca cgcgcgcgat ggatgggtcc aggtcaaagc tgagcaccgg catccaggca
     5581 tacaccttga cgtgggcgcg tgtctgcaac tgccacaccg cgcggttgaa cagattggcc
     5641 cgcacaggta gccaatggtt ggggaaatac accgatttga ccaggccatc ccccttcggg
     5701 tcggcgaacg cctgcaggaa cacggtgctc acgcccatgt cggcgatgcg ctgaaccaac
     5761 acccccaggt tcttgtccat ttgcgcagga ttcggatcgt agacatagtc gagatccact
     5821 tgcaccaccc gcatggaccg acgcgcctcg gtgctgagca cggtttccgc gaaatcggcc
     5881 atgccgggcg agtcggacac caggacgcga ggttctgaca tgagccggtt caccgtgccc
     5941 aagccatcat ccagtgtgag cgccatcttg tagccctgct tgcctatgat ggagatcgcc
     6001 tcgccgctgg ccgccccgta cggccacacc cacacgcgcg gcggcttgcc ggtgacttgg
     6061 cgaagcttct cggtgatgcg ccgggcatcg tcgcgaatgc gcttgcggta ggccgcgtcc
     6121 gactcatagg tatgcgtggc cgcgtcgtag cggtgcgtgg tttccgccgg ctggagattg
     6181 ccttccggat tggccgggat gccttcatgc tgggcgtatg tgtgcgaggc gatctcgacc
     6241 agacccgatc gcgacatttc gcggacctct tcccagttgg cgaaccgctt gcggggctcg
     6301 atgaccccac cgaaattcac ggtatggccg ggtggcgtgc tgacccaatc gcccaccggt
     6361 gcccacacag cgggccactc gaatgctttc aagatagggt agacgcgggt atagaagctt
     6421 ctgaagccat cgtcgaagct cagcagcaca gccttgggtg gcaggggttt gccaccctgg
     6481 tgtgcggcca ggatctgatc gaccgacaca ggctggtaac cgttgtcgtg gagccagtgc
     6541 atctgcgaca cgaagaggtc ggtgcgcacc gcaacgaaaa tctggttccg cccatggtcc
     6601 tcgatgtcgt gatacgccag cgtgagcaga tgattgaccg gccacggctt gtcgacgttc
     6661 gccacaggcc gttcggcggg cggcacgaac ttgggaatgt cgcgggcgca agccgccaga
     6721 agcagaacta cacatgccag gaacacaaag cgcaaacggg aaaggagaga tgacatggcg
     6781 tgcctctgcc tcaaaacctg tacgtcatgt cgacgacaat gctgtaatcg cgctctcgtt
     6841 ggccgtcata cggccgggac gtgcccgaca gcatgaaacc gacgtcgagc acgtcgttat
     6901 aacggaaacg ctgcccataa cccacggtga caatgcccgc tgtgccatga tcctgctgtg
     6961 aatacgcacc cgcccccacc aggaactgct gttcccatac ggtttcataa cggcgatgca
     7021 acacctgcgt gaaccgtagc gagggcagtg ccgtgaaatc ggacttggga ttgaaataag
     7081 gggtgttgtc cagggtattg tgcgatgccg agagatcaag cagagcgtcg attttcaggt
     7141 tcgggtccgt gtacaagcgc tggcgtccgg acagatcgag ttccattcgg ttgttgccat
     7201 cggtgaaatg cgacggtgtg aacgagaacg tccactcgcg ccgctcgtcg ccgcgccaac
     7261 gcacgtaagc attcaagctg ttcgaagtaa tgtcgtgctt caacgcccgc agtggggtat
     7321 tgcgcgacat caccgccgcg ccgccaccga cttgccaatg gtcgttgagg tcgaccgacg
     7381 ccgacagcgc ggcgcccgtg cgaatgccgt acccgaaact attgccggaa acctcggcct
     7441 gcgcagtcac atcgcggctg cgccattcgg cgcctgcacg catccaacga taattgcccg
     7501 aaccttcctc gaactcacca gtggaatacc ccatgcccgc gaagccgcgc cagttttcat
     7561 tgatgggcgg cgagtacaga acgctatcga tgttccagtc gtggctgccc atgaccggac
     7621 tgttggtgga cacgccgcgg ctggccgtga cgcgtaattc ggacatgttg tgcacgtccc
     7681 attcgcgggc caggcgctgc acggacatgt cctccgggcg gcgcgccacc acgtcgtcac
     7741 gcaggatctt tgcttgacgc cattcctgca agtcgagcgc cgtttcggcc tgattggttt
     7801 ccacgtcaac tgagcgcggc ccctcggttt ccgcgatttt gagatcgtgc tcggcgtgcc
     7861 ggggcagttc ccgtgctcga tagacctggg accgtgccgt gcgcagacgg gtgttgttgg
     7921 gcgcctcgtt caccatttga tcgagacgcg cctgagcctt cgacgcatgg ccctcgtaca
     7981 gataacccat ggctcgtgtg aggtcggcgt caagcctcag attattaggt tggcgactac
     8041 ggtcacccgg cacatagagc cataccggtt gttcggagac cgccgcgttc agttcagtat
     8101 tggcctgttt gaaattgccg gattccgtca gggcgtagaa caaccccgcc tggtcggcga
     8161 accgggtatc gggatcgtgg ctggcggccg gcgaagccag cgcgcgatgg aacaacgcgg
     8221 ccgccttctc tggttgacgc aggtataaat aagcggcggc cacatccccc aacacataat
     8281 ctgggaccgt cgtgccttga gccaacatgg cctcgtagct ggtgacgatg tccttcatac
     8341 gcatgcgcgc gtgtagcgcc aggatgcgat ccgcttcggc acgcacgaca tcggcgtgcg
     8401 ccgaggggcc cagtgccttc cattcgggga tgagcttgtc gtacatggcg agcgcacgat
     8461 cggccaactt gaaccggttg gcttcgcccc gtgacgaggc ggcggccacc cgggttagtt
     8521 gcgcggcgac atcggcctgc aacccacgga cctgcgccgc atccatcaca ccgggatggg
     8581 cctgggcctc gcgcagggcg gcctgggaga ggcgcgcgcg ctgcagcgcc aggatataag
     8641 cccgcaccac ataagccttg ccgggcgcca agccatatgc cttggtggct tgttcgagct
     8701 cggcataagg ctcgccgcca atatgctcgg cgtaggccaa ggcaaggcgg ctatcggcat
     8761 tgcgtggttc acgcgccacc agcttctggc ccagaccgac ggcttcatcg atgcgccggg
     8821 catcggccag cgtcatgatc cgacccagcg caaacccctg ttgagacgga aagcgacgct
     8881 ccccctctcg atacagggcg atggccttgt tccattgttt gtcgttgcga tacgcacggg
     8941 caaccgccaa tagcggctca gcgggcaggt tattcggcga cccggccgcc tcgtaggccg
     9001 ccgcgacttg gcggctatgc ccggcccaat cggatatcac gatatagtca tacagcaccc
     9061 ggcgatcgga aggcgcgctg tgtaggcgct ggctcaacat cgagagcgca ggctgataat
     9121 cgccgctgcg cgcctcacgg atcagcttgt cgtaacctgc gggggacgcc gcccacgaca
     9181 acgtcggcag agcggcgaag acggcgcaaa tcgcgatggt gacacgcgac gaatgaagcg
     9241 acatggcgat gctcgcaaat gacggagcgc cacgggcaaa gtggctgttc cgggcttagt
     9301 tcttgaacgc gactcctgtg cggtgggccg gacagcgcgc tgttaatgta agttatctag
     9361 acagaaccgt atctattcgt acgaagtggt attcaattac gtagatacaa accaggaaag
     9421 catcctacaa agaaaggcgg cagaccatat atcggccatt tggctggtac atagggcaag
     9481 tgaccgatgc tgggcacgga atccaagata ccgcatttcc acagaaaagc gcgaacaaaa
     9541 cccgcaattc tgtactgaca cccctttata ccgccagcca cgctggattt tgttgctttt
     9601 tgtcttatat caggggttac gacgaaacac caccgccatc attcagaaac cttgcgtgat
     9661 cgatgcgtca ccatccggtc gtatagccgg gagacgatgc cgctaccggt cttgacgaac
     9721 agaaatggga aaataccgtg aacgatggcg gccagcgccg cgccggccat tggaacgcca
     9781 aacgaaaaag acgccgccat gtgctggaaa tagctttcac caaccgacgc gggatgtgtc
     9841 gtgaacattt gcgtgaattt gctcatggtg ggcctcactc ttcatatttc aaaaaatgat
     9901 atacaaatta tatttgaata tataaagaaa aggctttcaa aaaccacgca ataaccgaca
     9961 taagtagtta ttatttttta taattcatat attaatagaa taattttcta taaataaaga
    10021 aaacaagaga aactcaaagc agtgccgcat gcctgaagac cagccattgc ccacatgtca
    10081 ctgaggcagg atgcgtcgcc gggacacatc agccggatcg gccggcgccg gcgtcactgg
    10141 cgtcgcaacc agctccagct tatgcgtttc gaaatccctg tgaacgggat ccagactggg
    10201 catggtcttg ttggccttgg ccgacaattc actgaagcgt gctgtcttgc catacagccc
    10261 ctgctggccc gcgaccgccg tcacggtatc gttgtaatgg cggctggcgg tgcccaatgt
    10321 gtcgcccagc cgcttcaggc gctcggccac cagcacgacc tggttgtaga tctcggccgc
    10381 cttggcgctg atctcgtggg cttgctcatt gctgcgcgcc accatccata gattggccac
    10441 cgttttcagg attggcatca gcgtcgtgtg cgacaccatg atgacgttct tttcgtaacc
    10501 gtagttgaac aaggacttgt tgtgtttgag cgcctcgatg taggcgggct cgatgggcat
    10561 gaacatcagg acgaagctgg ggctgtgcac accgatgaga ttgctgtagt ccttgccggc
    10621 aagatcgtcg atgtggttct tgatggcctt gacgtgcgcg tccagcgccg catcgaactc
    10681 ggcctgggta ccggccgcga tggcgcgatc gtaatccacc agcgagacct tgctatcgat
    10741 gaccagatgc ttttgatcag gcagcatgac cacgaaatcc ggctgccgag gttggccatc
    10801 gacgtccttg aaccggggct gtgactcata atgatcaccg cgcacgagcc ccgcctgctg
    10861 caaggcgagt tcgagctgga cttcgcccca gttgccagtg gttttcttgt cacccttgag
    10921 agccgtggcc agcgtgttgg cctccgtgct cattttcatg ccgatctcca gcacccgctt
    10981 gatctccaca cccagagaaa cattgccgcg caccgattcg tcgtgcactt gattgacacg
    11041 ccgctggaag ccttcgatct gctcgcggaa cggcttgagc agaacctcca aggacgcctg
    11101 gctgttggcg gcgagcgtct tgcccttttc ctccagaatc tgattggcca ggttctggaa
    11161 ctcggttttc agctgcagcc gggactggtc gaacgaagcc ttgagctctt cgaagtgcgc
    11221 cactttctgc ttgtacgtcg tgctcatctc ggcgagttcg cgattgacct cattcaatcg
    11281 gtcaccggtc gcgcgcaagg ccgcctcttt gtcagccagc gcggacttgg cttccgccaa
    11341 tcgttcccgc aggctggatg aatcggattc cgcccgcgcg agttggcgtt ccgcatggcc
    11401 tttttcggcg tcgagcgcaa tgctcttcgt gcgcgattga tcgaggtcgg cgcgcaatgc
    11461 ggtgatggtt tcaccgcgtt gcgtgttgct tgcctggctc agtgtgagct ctgtgcggat
    11521 tctcgccagg acacgccgga aacagaccaa cgtgacgagc acgcccacca gaagcccggt
    11581 cagtgccagc agccagcctg ccagcccggg aaccagcccg ggattcaatt cagtcacgac
    11641 aatcatccta taaacccggc cggacgcacg acgactccat gcgtcatcgc gaccgatgat
    11701 tttgtattgg tcagcgcatc gaattcatgt gtgtggggtt gtcgatgcac ccttgtctcc
    11761 gcattgaaat cgagccgaac gcaatcaatg gctggcccgg cccactgtgt gcagcggttg
    11821 tcaactatat cgttcagctt aggcaagcgg tcaagccgca agcgcaacat ttagttattg
    11881 aacattaccg acatccgcgg cccgcaagcc ggagacacac ctcgcgcgcg ccacagccag
    11941 tgctggcacc gctttgcgcg acaccccaat ttccacactg tcagtagggt ttgagatcgc
    12001 ggcctgtcgt ggttttgcga cgccgtacga aacgaaacgc cgaatcacgg cgatggccgg
    12061 tgctatcgcg aggattcgca acgtggcaga cggccgcaag ccgcagaggc atggtgacct
    12121 tgttcagagg atccctgagc acaggcttca ctttttcaca ccgaaacact ggtccagccc
    12181 cggaaacgtg cctgcgcgca cgtcggcggc gtattgttcc actgcggcgc gtatcaccgc
    12241 gcccacgtcc gcgaattgct tggcgaattt cggaatcttc gatccgctca ggcccaggat
    12301 gtcttccgtg accaggatct ggccatcgca gtcgggggat gcgccgatgc cgatggtggg
    12361 aatccgcaac gattctgtca tgcgtcgcgc caagccttca gcgataccct cgagcacgac
    12421 ggcgaaagcg ccggcctctt cgtgggcaag cgcgtcctgc aaaatgcgct cggccgattc
    12481 gtctgtcatg ccctgggcct tgaacccacc catggtgttc ttgtactgcg gcatcaaccc
    12541 aacgtgcgcc agcaccggc
//
LOCUS       DASTNK010000056        18798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_780429, whole genome shotgun sequence.
ACCESSION   DASTNK010000056 DASTNK010000000
VERSION     DASTNK010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 18798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18798
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780429"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..674)
                     /locus_tag="VFR20_04205"
     CDS             complement(180..674)
                     /locus_tag="VFR20_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEU0229750.1"
                     /translation="MPEIKLDRTDCRILNELQHNGRLNNQDLADRVSLSPSPCLRRVR
                     RLEEEGYIRQYVALVDSEKIGRGLLAYVAIRLNKSLRASHAPMSDFARDVQAWPEVVE
                     CYAMSGDMDYLLRIQVEDLTRFSRFAMDKLIQHPAVIDMRSSFMMQRIKETTELPIEV
                     LAGQ"
     gene            948..2057
                     /gene="hppD"
                     /locus_tag="VFR20_04210"
     CDS             948..2057
                     /gene="hppD"
                     /locus_tag="VFR20_04210"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEU0229751.1"
                     /translation="MTTPFQPWDNPMGTAGFEFIEYTAPDPAALGKVFESLGFKAIAR
                     HRTKNVTLYRQGGINFLINAEPNSFAQRFARLHGPSICAIALRVEDASSAYKRALELG
                     AWGFDSHSGPMELNIPAIKGIGDSLIYLVDRWRGKQGHHGIGDIGIYDVDFDPIDAST
                     ARQDLEHKGAGLTMVDHLTHNVHKGRMEEWAQFYERYFNFREVKYFDIEGKVTGVKSK
                     AMTSPCGQIRIPINEEGTEEKGQIQEYLDLYHGEGIQHIALATDNIYETIENLRQRGL
                     RFLDTPDTYYELLEGRIPQHGEDVARLRKNGILLDGTHDGGLLLQIFTENQIGPIFFE
                     IIQRKGNDGFGEGNFKALFESIELDQIRRGVVKAD"
     gene            complement(2163..2705)
                     /gene="msrA"
                     /locus_tag="VFR20_04215"
     CDS             complement(2163..2705)
                     /gene="msrA"
                     /locus_tag="VFR20_04215"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU0229752.1"
                     /translation="MEIPVHVETAILGGGCFWCTESVFASLKGVVDVTPGYAGGHVAS
                     PSYEQVCQGGTGHIEVSRVRFDPAVISFETLLQVFFATHDPTTLNRQGNDVGEQYASA
                     IFCQDDQQAVVARKVIAEVEQQIDAPVVTRVLGTGPFWPAENYHHDYYTRNPGQGYCQ
                     MVISPKLAKFRKRFAALLAG"
     gene            complement(2744..3091)
                     /locus_tag="VFR20_04220"
     CDS             complement(2744..3091)
                     /locus_tag="VFR20_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU0229753.1"
                     /translation="MSSKLYLLSLVLLACAPAASAAETTGLAAVESAHCMACHEVRRM
                     RVGPPFEAISRRFAGQRGAEEYLAHTIRSGGRGRWGAVPMPAQPQVTPEQAVVIAKWI
                     LSLSRAGSAADAQ"
     gene            3215..4012
                     /gene="lgt"
                     /locus_tag="VFR20_04225"
     CDS             3215..4012
                     /gene="lgt"
                     /locus_tag="VFR20_04225"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU0229754.1"
                     /translation="MLRYPHFDPIAVHIGPLAVHWYGLMYLLGFALAWVLGRWRINHG
                     KTDLTLADLEDIIFYGVLGVVVGGRLGYVLFYQPDLYFAHPKQIFYLWDGGMSFHGGL
                     LGVILVMLIFAHKKKRTLLEIGDFVAPLIPLGLAAGRFGNFINGELWGRPTDLPWGMI
                     FPGAHDNIPRHPSQLYEMGLEGFVLFAVLWWFARKPRPTGQISAVFLMGYGIARFLVE
                     FTRQPDYFLGLIFDGLSMGQLLSLPMIIIGAILFWVSARKSIPAPKP"
     gene            complement(3951..4691)
                     /locus_tag="VFR20_04230"
     CDS             complement(3951..4691)
                     /locus_tag="VFR20_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525304.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEU0229755.1"
                     /translation="MQKRKWLLGSVLMVAAGSLASTAFAHDDHEHKAHQSWPQATLQA
                     QASAVVSQDTVRITLASELTGASQTAVAQDLSKLLDGVMADAKKSSKVKVSSGNYRVW
                     PVSDKDGKISTWHGRGEIFLESTDFAAASKLAGQLSDRMPVANLDFFVSRALRDKEEN
                     ALLQGAVRAFRARAQALATALGYDGYRIREVRVGGGGGYVPVLSPRMMAMAAAPKADV
                     PLEAGTETITVSVQGSIFLRSPRKESHQ"
     gene            complement(4755..4935)
                     /gene="ssrS"
                     /locus_tag="VFR20_04235"
     ncRNA           complement(4755..4935)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFR20_04235"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(4992..5309)
                     /locus_tag="VFR20_04240"
     CDS             complement(4992..5309)
                     /locus_tag="VFR20_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU0229756.1"
                     /translation="MERIDVSILGHDYSLACAASEKDALLTAVRYVDQRMLGVKGSGK
                     VSSNERIAVMAAIQIAAELLAMRAPNGPLGDVALGDFKRRIDDMNSLLDQALEPAARV
                     AGR"
     gene            complement(5316..5720)
                     /locus_tag="VFR20_04245"
     CDS             complement(5316..5720)
                     /locus_tag="VFR20_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229757.1"
                     /translation="MLQDLDSLAARLGQLVQFTRQLQAERATLQTRLKSLEQERNALR
                     EQLGQRESEHQTVAQRLERHSAEIDAVRQDAERALAEMRAEVSRYQTEHRGLQEQLQA
                     CRSDSDHLRAVTSAARDSVDAILMRLPGAAQE"
     gene            5938..6843
                     /locus_tag="VFR20_04250"
     CDS             5938..6843
                     /locus_tag="VFR20_04250"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0229758.1"
                     /translation="MPSLNARDYQPVPPLNVAFLGLGIMGFPMAGHLVRAGHRVTVYN
                     RTAAKARAWVDEFGGTAAATPREAAANADIVFCCVGNDEDLRSVALGASGALAGMKPG
                     SVFADHTTASAEVARELYSAAGKLSVGFVDAPVSGGQAGAVNGVLTIMCGGEQRHFDI
                     IAPVAAAYGRAVTRLGDPGAGQLCKMVNQTCIAGLVQGLSEAIAFGQAAGLDMSLVLQ
                     VISKGAAQSWQMENRGATMIEGKFDFGFAVDWMRKDLGLVLSEARRNGARVPVTALVD
                     QFYADVQKMGGNRWDTSSLIKRLAD"
     gene            complement(6847..7407)
                     /locus_tag="VFR20_04255"
     CDS             complement(6847..7407)
                     /locus_tag="VFR20_04255"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEU0229759.1"
                     /translation="MLDREGYRPNVGIILVNQRNEVFWGKRIREHAWQFPQGGINYGE
                     SPVQAMYRELHEEIGLHPEHVRILGRTRDWLRYDVPENFIRRDSRGHYKGQKQIWFLL
                     RMVGRDCDVCLRATHHPEFDAWRWSQYWVPLDAVIEFKRGVYTTALNELSAILFRRGG
                     GETRYLRQRTQGGAARASGRGVRVPP"
     gene            7473..9206
                     /locus_tag="VFR20_04260"
     CDS             7473..9206
                     /locus_tag="VFR20_04260"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU0229760.1"
                     /translation="MRASRYHINTLKEAPAEAEVRSHQLMTRAGMIRKIAGGIYTYMP
                     MGLKVIKKIESVVREEMNNAGAIELLMPVVQPAELWQESGRWEKYGAELLRIKDRHQR
                     DFVLQPTSEEVITDIARNEIHSYRQLPINFYHIQTKFRDERRPRFGLMRGREFTMKDA
                     YSFDRTEVDAQASYDAMYAAYVRIFDRLGLTYRAVAADTGSIGGDRSHEFQVIADTGE
                     DLIVYNPDSQYAANIELAAAPCLIAARGPASEPLFKAPTPEASKCEIVAEQLGRPLTD
                     TVKAIVLATEPADGRGPAKIWLLLIRGDHELNEIKTGKLPGFENGHRFATEEEILATF
                     GCVPGYLGPVGIDASVPVIADTTVANMNNFICGANAEGFHYTGVNWGRDLPEPQVTDL
                     RNVIAGDPAPEGGSLAIQRGIEVGQVFFLGTKYSKALNATFLEADGKPALLQMGCYGI
                     GISRIAAAAIEQNSDDKGIIWPRALAPFEAVICPMGWNKSETVRNAAETLYAELREAG
                     VDVLLDDRDVRPGVMFADWELIGVPLRITVGDRGLKEGVVELQGRRDDAAATIPVAAA
                     AKAAIERLATL"
     gene            9249..9674
                     /gene="ybgC"
                     /locus_tag="VFR20_04265"
     CDS             9249..9674
                     /gene="ybgC"
                     /locus_tag="VFR20_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEU0229761.1"
                     /translation="MPLDTIAAQAELDIRVYYEDTDTGGVVYYANYLKFFERGRTEWL
                     RRLGVDQSDMARREHKVFVVKSVQIQYRRPARLDDLLTVRSAITRVGPASINFEQTAL
                     CDGELLCESAIQVCCVDQRTFRPAPFNRELRVLLEKVQN"
     gene            9677..10348
                     /gene="tolQ"
                     /locus_tag="VFR20_04270"
     CDS             9677..10348
                     /gene="tolQ"
                     /locus_tag="VFR20_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEU0229762.1"
                     /translation="MHPSSDLSLVTILLNASIPVQLVILVLAGISILSWTYIFSKRAT
                     IRRAAEQTHRFEDDFWAGGDLSVLQQAISSRRNEHGALARIFDAGMTEFVKARRNHNS
                     DAMLDGPRRAMQATYQREMDGLESHLNFLATAGSISPYIGLLGTVWGIMHAFIGLSAV
                     QQATLSSVAPGIAEALIATAIGLFAAIPAVIAYNRYTNQIDRLAIRFDSFIDEFLNIL
                     QRQVH"
     gene            10348..10800
                     /gene="tolR"
                     /locus_tag="VFR20_04275"
     CDS             10348..10800
                     /gene="tolR"
                     /locus_tag="VFR20_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HEU0229763.1"
                     /translation="MSIHHGHRRSRRLKSDINVVPYIDVMLVLLVIFMVTAPMITPGL
                     INLPSVGQAAEVPVKPIEVQIAENGDISIRVREAGGEFQRISPDSLLSEVQSRASADT
                     PVVIAADGKVPYETVMKVMDQLRSGGISRLGLLVDQKAGSNTPPTPTR"
     gene            10844..12094
                     /gene="tolA"
                     /locus_tag="VFR20_04280"
     CDS             10844..12094
                     /gene="tolA"
                     /locus_tag="VFR20_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HEU0229764.1"
                     /translation="MKQILKRAQNTAAPRTDEERDERRGLVISLGIHLGLVVLVFLGL
                     LGAPRNPNPVQIELWAQGTLPEAAQPDTANEPPDTSEQAQKQTPPKPPTPTPPPKPVP
                     QPKPTPTPPPKAQPAPPPKVAPPAPAPDENPDIALEKARKEKEKKEAEHKAAEQAAAA
                     KKAKEEAVKKAAEIKAEQEAAAKKAAELKAKQAAAKAAAEQKAQEAAEAKAEAEKAAK
                     EKAAKAKAEAAKKAAAKAAAEQKAQEEAEAQAEAEKAAKEKAAKEAKAKAEAAKKAKE
                     AAAKKAAAAKKAAEAKHQALMAAMRSAALGAAGIPNGTADHNQAGGGGNDNGYAAKVR
                     ACIRPRVAYPVPPRSGSNPTVQYRANLSPKGLVESVEVKRSSGIPGFDQAVVKGIKSC
                     SPFPKPPSGRYPSYIDGNYLMYDN"
     gene            12110..13420
                     /gene="tolB"
                     /locus_tag="VFR20_04285"
     CDS             12110..13420
                     /gene="tolB"
                     /locus_tag="VFR20_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEU0229765.1"
                     /translation="MTAFTLDGTTTRLGWRPWACALLMAGASLAGIRSAHAQLRVDIS
                     GVGATQYPIAIADFSGNPQGQAIANVIRADLTRSGQFQLINATGANLTAESTIAYNDW
                     RTRGADYLAYGTVTQSGDTYNVSYRLADSVRQSQLDGVAFTGTSKELRRISHQIADRI
                     YEKITGVRGVFSTRLAYVLQRGNTYELQIADADGQNPQVMLRSKHPIISPAWSPDGKR
                     LAYVSFETGKPVVYVQTLATGARLPVANFKGNNSAPAWAPNGQQLAIALSRDSISQIY
                     TINSDGSGLRRVMRSPLIDTEPNYTPDGQSIVFTSDRGGTPQIYKVPVTGGEAQRLTF
                     NGSYNTSPHVSPDGKQLVYVSRQDGSYRIALQDLASGSPTLLTAGPDDESPSFAPNGM
                     QILYSSIQGGRSVLAVVSVDGRVRQTLSALDGKVQEPTWGPFTN"
     gene            13458..13955
                     /gene="pal"
                     /locus_tag="VFR20_04290"
     CDS             13458..13955
                     /gene="pal"
                     /locus_tag="VFR20_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEU0229766.1"
                     /translation="MSSRIVKTLTVAAFAATLAACSSVPLNNNSAGANGAGNGATAGQ
                     VMDPFNPQSPLYQQRSVYFDFNKYNVKDEYRPLVEMHAHYLVAHPSQKVQVQGNTDAR
                     GSAEYNLALGQRRSEAVARMMTLLGVKSDQIEAISYGKERPKATGSTEADYAENRRAD
                     IVYQH"
     gene            14031..14720
                     /gene="ybgF"
                     /locus_tag="VFR20_04295"
     CDS             14031..14720
                     /gene="ybgF"
                     /locus_tag="VFR20_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEU0229767.1"
                     /translation="MNVKALSLRPVLLATAVSFAALCAAPAHAFSDDEARRAILELRS
                     QVQQLIQQNQQARLQLADQIESLQNEVTDMRGQIEKLRWEADISKRSSQDQSGGATPS
                     VTDPKEQAAYDQPMGLFRSGKYKEAATGFAAFIKNFPNSQLAPEAQFYEGSSRYASRD
                     FKASIAELQSLVHSNPKDARAPDALLIIAASQIELNNVAGAKSNLQKIVKDYPDSSAA
                     DTAKSRLKLLK"
     gene            14720..15760
                     /locus_tag="VFR20_04300"
     CDS             14720..15760
                     /locus_tag="VFR20_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU0229768.1"
                     /translation="MPGEARTAPTPPARYARQRNLVILAGALAALLCANLLTGPAPIG
                     FDALRHVVLNPGDHSTTAIIVRQIRLPQALMALLTGAALGLSGAEMQTILDNPLASPF
                     TLGVSSAAALGAALAIVLNLSVPWVPDPYIVAVNAFVFALACTALLHLAARHARTGTA
                     GIILLGIAMVFTFNALVSLVQFSASATALQDLTFWMMGSLARSDWPRLGVAALALCVI
                     APFSLRSAWALTALRFGDERAASFGVDTRRLRRAALVRTSLLAAVAVSLTGVIGFIGL
                     TAPHIARRLWGEDHRWYLPASALTGSAILLAAAIVSRLGGGGVVIPIGIVTTLVGIPF
                     FCVAVLRRRPEA"
     gene            15757..16551
                     /locus_tag="VFR20_04305"
     CDS             15757..16551
                     /locus_tag="VFR20_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0229769.1"
                     /translation="MNTSGFATLRLRALNVAFGATRVFDNLTLPDLSAAGITALLGPN
                     GCGKSTLLKSIAGLVPATCHALTLGASDLKGLDAAARSKFVRYLPQAAFKDVHLTVRE
                     AVTVAGKIRRSAAERRLDDVVDETLRDLGLAALSHRYLDELSGGQKQLVGLAQALIHQ
                     PTLLLLDEPLASLDPNHQIHVMSMLTRLASERAVAVIVVLHDLDMALRFADHIWLLRG
                     GRLIAAGIPADVISSANIAQAFGVTARVENDSLGQPHVFVDDPIQL"
     gene            16564..17382
                     /locus_tag="VFR20_04310"
     CDS             16564..17382
                     /locus_tag="VFR20_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0229770.1"
                     /translation="MPHDIDLPQSVADGMSYPLAYTESGSGAPLLLIHGSLCDYRYWR
                     WQIPAFGQAYRTLAPSLRGYWPAAFRSERADFDVLVQTRDLIEFLDHTSPDGTPVHVL
                     GHSRGARVALELACAAPEKVRTLTLADPGFSPVGQAGPVPFQIEAARRLSDGDTDGAL
                     EVFIDTVSGSDTWRHMVGWFKAMVRDNAGTLLSQIKERHHEFDMARAGAISCPTLLLT
                     GSDSPARFGRVLASLEQVLPHVEKTTIPLASHGMNLANPRVFNQHVLGFLGSHS"
     gene            complement(17389..17943)
                     /locus_tag="VFR20_04315"
     CDS             complement(17389..17943)
                     /locus_tag="VFR20_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEU0229771.1"
                     /translation="MKKHVLEGFLVVCGALFAGVALAGGTSMSGDMPMSGHMSMKGGA
                     PMPHADHGEAGAMQSSTTLQVSGCWIRALPSPAPSAGYFVVHNNGAHAAVLTSAASPA
                     FGMVMLHKSVESGGVSKMIMESSIPVPAGGSLTFKPGSYHAMLEQPVHKLVVGSTLGL
                     ELRFASGEVARATCLVRPAGALSR"
     gene            18080..18550
                     /locus_tag="VFR20_04320"
     CDS             18080..18550
                     /locus_tag="VFR20_04320"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU0229772.1"
                     /translation="MKTSPSPDTPHITVAEFIQLLEREHPFAAVLGLDVLEIGHGTAL
                     LRLPDRDDHKRLGGIVAGPMLMGLADLALYAAVVGATGNTHAVTASLTINFMRKAPAG
                     GILAHARILKTGRLTAGEVLLTPTDGGDALAQVVSTWTLPGPRSARPAAMPATA"
     gene            18557..>18798
                     /locus_tag="VFR20_04325"
     CDS             18557..>18798
                     /locus_tag="VFR20_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229773.1"
                     /translation="MTQPPTAVRASDTTPVTATRRPTPDRRKPAKPWRSEVKASMSLG
                     WPIILTNLAQLAILTTDVILIGRLGPEPLAAGSLSI"
BASE COUNT         3827 a         6154 c         5455 g         3362 t
ORIGIN      
        1 ctacccgctt cgggccgcca aacatggtgc gctcaatcat tgaaacacca ccaccctcga
       61 ccgccccggc cttcgatcaa cccggctcct agcgccgcgt tcatcaatca ccgagccaag
      121 aattagaaca caccaccttt gaatgtgtca aatcaatgcg ccggccccat gattcatgat
      181 cactgaccag caagaacctc gattggcagc tccgtggttt cttttatgcg ttgcatcatg
      241 aagctcgagc gcatgtcgat cactgcggga tgctggatca gtttatccat ggcaaagcgg
      301 gagaaccggg tcagatcctc cacctggatg cgcagcaggt agtccatgtc acccgacatc
      361 gcgtagcact ccaccacttc aggccaggct tgtacgtctc gcgcgaagtc ggacatcggg
      421 gcatggctgg cgcgcagact tttgttcagg cgaatggcca catacgccaa caggccgcga
      481 ccaatttttt ccgaatcgac aagtgccacg tactggcgga tatagccctc ctcttccagt
      541 cttcgcaccc ggcgcaggca cggactcggg gagagcgata cccgatcagc aaggtcctga
      601 ttgttcagcc ggccattgtg ctgcaattca ttcagaattc tgcaatctgt tctatctagc
      661 tttatttctg gcatatgaca cccaatcaat cgacagttac ggcaatatta ttgctcatag
      721 ttcagttatt tcagcaacat tagcaatcgt ctgccccgga tttatacata gaattattca
      781 aaatgaacgt cttgctccgc acgttgccat gcctttcctg ccggcatcgc aaaccagcgc
      841 ggaccgcatg actggttcgt tcattttgaa atcaatcaaa cagcacacca acccgccccc
      901 caggatccac gacggcgcca gggcccttta ccggagagac attcatcatg accacacctt
      961 ttcaaccctg ggacaacccc atgggcaccg ccggctttga attcatcgaa tacaccgccc
     1021 ccgaccccgc cgcgctgggt aaagtgttcg aatccctggg attcaaagcg atagcccggc
     1081 accggaccaa gaacgtcacg ctgtaccgtc agggcggcat caactttctg atcaatgccg
     1141 agcccaactc cttcgcacag cgcttcgccc gcctgcacgg cccctccatt tgcgcgattg
     1201 cgctgcgcgt ggaagatgca tccagcgcgt acaagcgcgc actggaactg ggtgcctggg
     1261 gcttcgattc acatagcggc cccatggaac tgaacatccc cgccatcaag ggcattggcg
     1321 attccctcat ctatctggtg gatcgctggc gcggcaagca gggacaccac ggcattggcg
     1381 acattggcat ctacgacgtc gatttcgacc ccatcgacgc ctccacagcg aggcaggatc
     1441 tggagcacaa aggcgccggc ctgaccatgg tcgatcatct gacccacaat gttcacaaag
     1501 ggcgcatgga ggaatgggcg caattctacg agcgctattt caacttccgg gaggtgaagt
     1561 atttcgatat cgaaggcaag gtcaccgggg tcaaatccaa ggccatgacg tcgccctgtg
     1621 ggcaaatccg catccccatc aacgaggaag gcaccgaaga gaaaggccag atccaggaat
     1681 acctggatct ctaccatggc gagggcattc agcacatcgc cctggccacc gacaatatct
     1741 acgaaaccat cgagaacctg cgtcaacgcg gcctgcgctt cctggacacg cccgacacct
     1801 attatgagtt gctggagggg cggattcccc agcacggcga agacgttgcc cgtctacgca
     1861 agaacggcat cctgctcgac ggcacacacg acggtggcct gctgctgcag atcttcactg
     1921 agaaccagat cgggccgatc ttctttgaaa tcattcagcg caaaggcaac gacggctttg
     1981 gcgagggcaa cttcaaggcg ctgttcgaat cgatagagct ggatcagatt cgccgcggcg
     2041 tcgtcaaggc ggattgagcc gcatcgggcc gcctgccccg cttgagaggc aggcgcgggg
     2101 cgccacccgc cgtgcgcagg cccgtgagaa gggacaccgg cgcggcatgg ccgactggtt
     2161 tttcagccag ccagtagcgc cgcgaaccgt ttgcggaatt tggccagttt gggcgaaatg
     2221 accatctgac agtagccttg acccgggttg cgggtgtagt agtcgtggtg ataattttcg
     2281 gccggccaga atggcccagt gccgagcacc cgggtcacca ccggcgcgtc gatttgttgt
     2341 tcgacttcgg cgatcacctt ccgcgccacc acggcctgct ggtcatcctg acagaagatg
     2401 gccgacgcgt attgttcccc cacgtcattc ccctgccgat tcaaagtggt ggggtcgtgt
     2461 gtggcgaaga acacctgcag caacgtttca aaactgatga cagccgggtc gaatcgcacc
     2521 cgggacactt ctatatgccc ggtgcccccc tggcaaacct gctcatacga gggggaggcc
     2581 acatgcccgc ccgcatagcc aggggtgaca tcgaccaccc ctttcagcga agcgaatact
     2641 gattcggtac accagaaaca gccgccgccc aggatggcgg tttcaacgtg gacagggatt
     2701 tccatggcga ggcacctcga cgattgacgc aaccatcata gcgtcattgc gcgtccgccg
     2761 ccgacccagc cctgctgagc gacagaatcc atttggcaat gacaacggcc tgctcgggcg
     2821 tgacttgcgg ctgcgccggc atcgggacgg caccccaccg cccgcgaccg ccgctgcgaa
     2881 tcgtgtgcgc caagtattcc tcggcacccc gctgaccagc gaaacgccgg gagatcgcct
     2941 cgaatggcgg accgacccgc atgcgccgca cttcgtggca cgccatacag tgcgcggatt
     3001 ccaccgctgc gagccctgtg gtctcggcgg ccgacgccgc cggcgcgcag gcaagcagta
     3061 caagcgacaa gaggtatagt ttcgaactca tgaaccgatt tcccgtcata cgaaggcagc
     3121 ccgtgcccgg tatgggccac gggctgcctc atgcggcgtg tcacccctga cgcgctgtat
     3181 tatcgacgat atttcattcg gaaagtttct ctccatgctc cgttatccgc atttcgaccc
     3241 catcgccgtg cacatcggtc cgctggccgt gcattggtat gggctgatgt atctgctggg
     3301 cttcgcgctt gcctgggtcc tgggccgatg gcgcatcaat catggcaaaa ccgacctcac
     3361 actggccgac ctggaggaca tcatcttcta tggtgtgctg ggcgttgtgg tgggcgggcg
     3421 cctgggctat gtgttgtttt atcagcccga cttgtacttc gcgcacccga aacagatctt
     3481 ctatctgtgg gatggcggaa tgtctttcca cggtgggttg ctgggagtca tcctggtcat
     3541 gttgatcttc gcgcacaaga agaaacgcac cctgctcgag atcggcgact tcgtcgcacc
     3601 gttgatcccc ctgggattgg cagcgggccg tttcgggaat ttcatcaacg gtgaattgtg
     3661 gggccgcccc acggatttgc cgtggggcat gattttcccg ggagcccacg acaatatccc
     3721 ccgccacccc tcgcaactgt acgaaatggg ccttgagggc tttgtgctgt tcgccgtgct
     3781 ttggtggttc gcgcgcaaac cgcggccgac cggccagatc agtgccgtgt tcctgatggg
     3841 atacggaatt gcgcggttcc tggtggagtt cacccgtcaa cccgattact ttctcggcct
     3901 gatcttcgac gggctgtcga tggggcagct gttatcgctg cccatgatca tcattggtgc
     3961 gattcttttc tgggtgagcg caagaaaatc gatccctgca ccgaaaccgt gatggtctcg
     4021 gttcccgcct cgagcggcac atcggccttg ggggcggcgg ccatggccat catgcgtggc
     4081 gagaggacgg gaacatagcc gcccccgccg ccaacgcgta cttcgcggat gcgatagccg
     4141 tcatagccca acgccgtggc cagcgcctgg gcacgcgcgc ggaacgcacg gacagcgccc
     4201 tgcagcaacg cattttcttc cttgtcccgc aaggcccgcg acacaaagaa atcaagattt
     4261 gccacgggca tgcgatcgct gagctggccg gcgagtttcg aggcggctgc gaaatcggtg
     4321 gattcgagga agatctctcc gcgcccgtgc caggttgata tcttgccgtc cttgtcggac
     4381 accggccaca cacggtaatt accgctactg accttgacct tggaggattt cttcgcgtcg
     4441 gccatgacac catcgagcag cttgctcaag tcttgcgcga cagctgtctg ggacgccccg
     4501 gtcagttcgg atgccagcgt gatgcggacg gtgtcctggc tgacaacggc cgatgcctga
     4561 gcctgcaagg tggcctgagg ccacgattga tgcgccttgt gctcgtggtc gtcgtgcgcg
     4621 aaagccgtgg atgccaaaga acccgcggca accatgagca cgctgcccaa caaccattta
     4681 cgtttttgca tagagaaaaa cattttcacc ccccaacaaa gcaagagccc gctcttgcga
     4741 gcgggctctt cgagaaatgc cccgccattg ccgtcaaggc cggcatccca gaaccgaggt
     4801 tcaaggtggt cgccatcagt caggcttcgg gttcgtcagg aaagacgatc acacctgctt
     4861 gtcaatccgg caacctaatg ccataagcat tggttcaaga aatgtatggc cgagtcacga
     4921 acacggcagg gactttcggt tgagcgccat gccggaacac ggcgcccgaa aactttcact
     4981 tcaccacgac ctcatcggcc tgccacacgg gccgccggct cgagcgcctg atcgagcaag
     5041 gagttcatgt catcaattct acgcttgaaa tcacccagcg caacatcgcc cagcgggcca
     5101 tttggcgcgc gcatggccag aagctcggcc gcgatctgga tggccgccat cacggcgatg
     5161 cgttcgttgc tggatacttt gccggagccc ttgacaccca acatgcgttg atcgacatag
     5221 cgcacggctg tcagcaacgc gtctttttcg ctggccgcgc aggccaatga ataatcgtgg
     5281 ccgagaatgg agacatcgat acgttccatg attcgctact cctgagccgc gcccggcaac
     5341 cgcatgagaa tggcatcgac gctgtcccgg gccgcgctgg tgacggcgcg caggtgatcg
     5401 gagtcggagc ggcaagcctg cagttgctcc tgcaggccac ggtgttcggt ttgatagcgg
     5461 gagacctccg cgcgcatttc agcaagcgcg cgctcggcat cctggcgaac cgcgtcgatc
     5521 tcggcggaat gccgctccag gcgctgcgcc acggtctgat gttcggattc ccgctggccg
     5581 agctgctcac gcaacgcatt gcgttcctgc tcgaggctct tcaggcgcgt ttgcaacgtg
     5641 gcccgctcgg cctggagctg acgcgtgaac tgcaccagtt gccccaatcg ggcagcgaga
     5701 gagtcgagat cttgcagcat gggcgttatc gtaaaccatc cccaccaaca acgggtgaag
     5761 ggtgtgtatt ccaagcggag tgaagccgcc atcataccgc gacatttcgg gtttcgcgca
     5821 aaaccggcgc gcgcaaggcg gccgttgcgg taatctaatg ggccagcgtt ccaagcggcg
     5881 ggctacccgc ccgccgcgct ctgaaccgaa ccaagttttc aaccggagac ccaagtcatg
     5941 ccctcgctca acgcccgaga ttatcaaccc gtcccgccac tcaacgtcgc cttcctggga
     6001 ctgggcatca tgggatttcc catggctggc catctggttc gcgccggcca ccgtgtcacg
     6061 gtgtacaacc gcacggccgc caaggcccgt gcctgggtcg atgaattcgg cggcacggcc
     6121 gccgcgacac cccgcgaagc ggctgcgaac gccgacattg ttttctgctg tgtcggcaac
     6181 gacgaagact tgcgcagcgt cgcgctcggc gcttcgggcg ccctggcggg catgaaacct
     6241 ggctccgtgt tcgcggacca caccacggcg tcggctgagg tggcgcgcga gttgtatagc
     6301 gccgccggga agctgagcgt gggatttgtg gacgcgccgg tatcgggcgg gcaggcgggg
     6361 gccgtcaacg gtgttctgac catcatgtgt ggtggcgaac agcgccactt cgacatcatc
     6421 gccccggtgg cggccgccta cgggcgcgcg gtgacccgac tcggcgaccc cggcgccggc
     6481 caactatgca aaatggtgaa ccagacatgc atagcggggt tggtccaggg gctctccgag
     6541 gccatcgctt tcggccaggc ggccggactc gacatgagtt tggtccttca agtgatcagc
     6601 aaaggcgcgg cgcaaagctg gcagatggaa aaccgcgggg cgaccatgat cgaaggaaaa
     6661 ttcgatttcg gtttcgccgt ggattggatg cgcaaggacc tgggactggt gctgagcgaa
     6721 gctcggcgca acggcgcccg cgtcccggtc accgccctgg tcgaccagtt ctatgcggac
     6781 gttcagaaaa tgggcggtaa ccgctgggat acatcgagcc tgatcaagcg gctcgccgac
     6841 tgatgctcac ggcggcacac gaacacctct gcccgacgcg cgcgcagccc cgccttgcgt
     6901 gcgctgtctc aggtatcggg tttcaccgcc gcctcgacga aacaggatgg ccgacaattc
     6961 attgagcgcg gtggtgtaga cgccgcgctt gaattcgatc accgcgtcca ggggcaccca
     7021 gtactggctc cagcgccaag cgtcgaattc gggatgatgc gtggcccgta ggcacacatc
     7081 gcagtcgcgg ccaaccatgc gcaacaggaa ccagatctgt ttctggccct tgtagtgccc
     7141 gcgggaatcg cggcgtataa aattttccgg cacatcgtaa cggagccagt cgcgcgtacg
     7201 ccctaatatg cgaacgtgtt cgggatgcag gccaatttct tcatgaagct cgcggtacat
     7261 ggcttgaacc gggctttcgc cgtagttgat gccaccctgc ggaaactgcc atgcatgttc
     7321 ccggatacgt tttccccaga aaacctcgtt cctctggttt acaaggataa tgccgacatt
     7381 aggacggtag ccttcacgat cgagcatggg ccacctcaga agcgaattca atacaattac
     7441 accgattata cgtatctcac gagatcccta ccatgcgtgc atccagatat cacataaata
     7501 cattgaaaga ggcccccgcg gaagccgaag tccgcagcca ccagctcatg actcgggccg
     7561 gaatgatccg caaaatcgcg ggcggcatct acacctacat gccgatgggg ttgaaggtga
     7621 tcaagaaaat agaatcggtg gtgcgcgaag agatgaacaa cgccggcgcc atcgaactgc
     7681 tcatgccggt ggttcagcca gcggagcttt ggcaggaatc gggccgttgg gaaaaatacg
     7741 gtgcggaact gctgcgcatc aaggatcgcc atcagcgcga tttcgtgctc cagcccacgt
     7801 cggaagaagt catcaccgac attgcccgca acgaaatcca tagctaccgc cagcttccca
     7861 tcaatttcta ccacatccag accaaattcc gcgacgaacg ccgcccccgc ttcggcctga
     7921 tgcgcgggcg cgaattcacc atgaaggacg cctactcgtt cgatcgcacc gaagtggatg
     7981 cccaagccag ctacgacgcg atgtacgccg cctacgtgcg catattcgac cgtctcggac
     8041 tgacctaccg cgccgtcgcg gcggacaccg gctccattgg cggcgatcgc agccacgagt
     8101 tccaagtgat cgccgacacc ggcgaagatc tgatcgtcta caaccccgac tcgcaatacg
     8161 cggccaatat cgagctggcc gccgcgcctt gcctgatcgc ggcacgcggt cccgcaagtg
     8221 aaccgttgtt caaggctccc acacccgagg cgtccaaatg cgagatcgtg gccgagcaac
     8281 taggtcggcc cctgacggat accgtcaagg ctatcgtact ggccaccgag cctgcggatg
     8341 ggcggggccc cgccaaaata tggttgctgt tgatccgcgg cgatcatgaa ctgaatgaaa
     8401 tcaaaacagg gaagcttcct ggttttgaaa acggccaccg cttcgccacc gaagaagaaa
     8461 tcctcgccac attcggctgc gtgccggggt acctgggacc ggtcggtatt gatgcgtcgg
     8521 tgccggtgat cgccgacacg accgtcgcca acatgaacaa cttcatttgc ggcgccaacg
     8581 ccgaggggtt ccactacacc ggcgtgaact ggggccgcga tctgcccgag ccccaggtta
     8641 ccgacctgcg caacgtgatc gcgggcgacc ccgcgccgga aggcggatcg ctggccattc
     8701 aacgtggcat cgaggtcggg caggtttttt tcctggggac caagtactcc aaggcgctga
     8761 atgccacctt cctcgaggcc gacggtaaac ccgcgctgct gcagatgggt tgctacggaa
     8821 ttggcatcag ccgcatcgcg gcggccgcca tagaacaaaa cagcgacgac aagggcatca
     8881 tctggccacg cgccctcgct cccttcgagg ccgtgatctg ccccatgggc tggaacaaaa
     8941 gtgaaacagt tagaaacgca gcggaaacgc tgtacgccga actacgtgaa gccggcgtgg
     9001 atgtcctgct cgatgaccgc gacgtgcgcc ccggtgtgat gttcgcagac tgggagctca
     9061 taggcgtgcc gctgcgcatc acggtcggtg atcgcgggct caaggaaggg gtcgtggaac
     9121 tgcaaggcag gcgggatgac gcggccgcca caattcctgt ggccgcggcg gcaaaagctg
     9181 cgatcgaacg actggccacc ctatgacagc gtgtcggcgg ctggccgaca caccgcttaa
     9241 ctaaactcat gccacttgat acaatcgccg cgcaggccga actggatatt cgcgtatatt
     9301 acgaggacac cgacacgggc ggtgtcgtct attatgcaaa ctacctcaaa ttcttcgagc
     9361 gcgggcgcac cgaatggttg cgccgactgg gtgtcgacca gtccgacatg gcccggcgcg
     9421 aacacaaggt attcgtggtc aagtcggttc aaatacaata ccgccggccc gctcggctcg
     9481 acgatctgct cactgtgcgc agcgccatca cgcgggtcgg cccagcatcc ataaatttcg
     9541 aacaaaccgc actgtgcgac ggggaactgc tctgcgaaag cgcaatccaa gtttgttgcg
     9601 tggaccagcg cacgttccgt ccggcgccct tcaaccgtga actcagggtt ttattggaaa
     9661 aggttcagaa ctaatcatgc atccctctag cgacttgtcg ttggtgacca tcctgctcaa
     9721 cgccagtatt ccggtgcagc tggtgattct ggtactggcg ggaatatcga tcctgtcctg
     9781 gacctacata ttcagcaaac gcgccactat ccggcgcgcc gccgaacaga cccaccgctt
     9841 cgaagacgac ttctgggcgg gcggcgatct gtccgtgctg caacaagcga tctccagccg
     9901 ccgcaacgaa cacggggcgc tcgcgcgcat cttcgacgcc ggcatgaccg aattcgtcaa
     9961 agcccgccgc aaccataaca gtgatgccat gctcgatggc ccgcgacgcg ccatgcaggc
    10021 gacctatcag cgggaaatgg acggtctgga atcgcacctc aatttcctgg ccaccgctgg
    10081 gtccatcagc ccgtacatcg gccttctggg caccgtctgg ggcatcatgc acgccttcat
    10141 cggcctgtcc gcggtacagc aggccacgct gtcgtcggtc gccccgggta tcgccgaagc
    10201 gctcatcgcc acagccattg gcttgttcgc ggccatcccg gcggtgatcg cctacaaccg
    10261 atacaccaac caaatcgatc ggctggcaat ccgattcgac agcttcatcg atgagttcct
    10321 gaacatcctg cagcgacagg tgcactgatg tccatccacc acggccaccg ccgttcgcgt
    10381 cggctgaaga gcgacatcaa cgtcgtccct tatatcgacg tcatgctcgt gctgctcgtc
    10441 atcttcatgg tgaccgcacc aatgatcacc cctggcctga tcaacctgcc ctcggtcggc
    10501 caggccgctg aagtgccggt gaaacccatc gaggtccaga tcgccgagaa cggcgatatt
    10561 tccatccggg tgcgcgaagc tggtggcgag ttccagcgca tcagccccga cagcctgctc
    10621 agcgaagttc aatcacgcgc atccgccgac acaccagtgg tcattgccgc tgacggaaag
    10681 gtcccctacg aaaccgtcat gaaggtgatg gaccaattgc gatcgggagg catcagccgg
    10741 cttggtctgc tggtcgacca gaaggccggc tcgaacacgc cgccgacccc aacgcgatga
    10801 tgccgcgcag gccgcgacac tctgcgggct cccccctcaa accatgaagc agatactgaa
    10861 acgcgcccag aacaccgccg cgcctcgaac cgatgaagaa cgggacgagc gccgggggct
    10921 tgtgatttcc ctgggtatcc acctaggcct ggtcgttctc gtgttcctgg ggctgctcgg
    10981 cgcgccacgc aaccctaatc cggtgcagat cgaactctgg gcacagggaa cgctgcccga
    11041 agccgcccaa cccgatacag cgaacgaacc gccagacacc tcggagcagg cacagaagca
    11101 aacaccgccc aagccgccaa cccccacgcc acctcccaag ccggtgccac agccgaagcc
    11161 aaccccgacg ccgcctccca aagcgcaacc ggcgccaccg cccaaagtcg ccccgccggc
    11221 tccggcgccc gacgagaatc ccgacatcgc attggagaag gcgcgtaagg aaaaggaaaa
    11281 gaaggaagcc gaacacaagg cggccgaaca ggccgcggcc gccaagaagg ccaaggaaga
    11341 agccgtgaag aaggccgctg agatcaaggc ggaacaggaa gcagccgcga aaaaggccgc
    11401 cgagctaaag gccaagcaag ccgccgccaa ggccgcggcc gaacagaaag cccaggaagc
    11461 agccgaggcg aaggccgaag ccgaaaaggc agccaaggaa aaggctgcca aggccaaggc
    11521 tgaagccgcc aagaaagccg ccgccaaggc tgcggccgaa cagaaagccc aggaagaggc
    11581 cgaggcgcag gccgaagccg aaaaagcagc caaggaaaag gcggccaagg aagccaaggc
    11641 caaggccgaa gccgccaaga aagccaagga agccgccgcg aaaaaagcgg ccgccgcgaa
    11701 aaaggccgcc gaagcgaaac accaggcgct gatggccgcc atgcgcagcg cggcgctggg
    11761 ggcggcgggc attcccaacg gaaccgccga tcacaaccaa gccggcggag gcggcaacga
    11821 caatggctac gcggcgaagg tcagggcgtg catacggccg cgcgtcgcct atccggttcc
    11881 gcctcgtagc ggatcgaacc cgacagtaca atatagagcc aacctcagtc cgaaaggact
    11941 ggtcgaaagc gtggaagtca aacgctcctc cggcattccc ggattcgacc aggccgtggt
    12001 gaaaggcata aagagttgct cgccctttcc caagcccccg tccggcaggt acccgtcgta
    12061 tatcgacggc aactacctca tgtacgacaa ctaacaggag attgcagtca tgaccgcttt
    12121 cactctggac ggcacgacaa cccgacttgg ctggcggccc tgggcctgcg cactgctcat
    12181 ggccggtgcc agcctggcgg gtatccggtc ggcgcacgcc cagttgcgtg tcgacatttc
    12241 cggtgtcggt gccacccagt accccatagc catcgccgat ttttccggca acccgcaagg
    12301 ccaggccatc gccaacgtca ttcgcgccga tctgacccgc tcggggcagt tccagctcat
    12361 caatgcgacc ggcgccaacc tcaccgccga aagcaccatc gcctacaacg actggcgcac
    12421 ccgcggtgcc gactaccttg cctacggcac cgtgacgcag tcgggtgata cctacaacgt
    12481 gagctatcga ctggccgatt ccgtgcgcca gagccagctc gacggcgtcg ccttcaccgg
    12541 cacgtcaaaa gaattgcgcc gaatctcgca tcaaatcgcc gaccgtatct acgaaaagat
    12601 caccggagtg cgtggcgtgt tctcgacccg gctcgcctat gtgttgcaac gcggcaacac
    12661 ttatgagtta caaattgcag acgccgacgg gcagaatccg caggtcatgc tacggtccaa
    12721 acaccccatt atttcacccg cgtggtcccc cgacggcaag cgtctggcgt acgtcagttt
    12781 cgaaaccggc aaaccggtcg tatacgtgca aacccttgcc actggtgcgc gtctgccggt
    12841 cgccaacttc aaaggcaata acagcgcgcc cgcctgggcg cccaacggcc aacagctagc
    12901 aattgcgctg tcccgcgaca gtatctctca aatctacacc atcaattccg acgggtcggg
    12961 gctgcgtcgc gtcatgcgtt ctcccctcat cgacaccgag cccaactaca ccccggatgg
    13021 gcagtcgata gtgttcacca gcgatcgtgg gggtacgccg caaatttaca aagtgccggt
    13081 aactggtgga gaagcccaac gcttgacctt caatggaagt tacaatacgt caccccacgt
    13141 ctcgccagat ggcaagcagc tcgtgtacgt tagccgacaa gatggttctt accgaatcgc
    13201 cctgcaggac ctggcttcag gttcgcccac gttgcttacc gcgggcccgg atgacgaatc
    13261 acccagcttc gcacccaatg gcatgcaaat cctttacagc tctattcaag gcggtcgcag
    13321 tgttctggct gtcgtgtccg tcgacggccg cgtacgccag accttgtccg ccctggatgg
    13381 aaaagtgcag gagccgacat ggggtccgtt taccaattga ttttcacagt gtgactcttt
    13441 ttcaaaggaa atcccaaatg agctcgcgca tcgtcaaaac cctcaccgtg gccgctttcg
    13501 ccgctacgct cgcggcctgc agctccgtgc cgctcaataa caattcggcc ggcgcgaatg
    13561 gtgccggaaa cggagcaacc gccggccagg tgatggatcc gttcaacccg caaagcccgc
    13621 tgtatcagca acggtccgtg tacttcgact tcaacaagta caacgtcaag gacgaatacc
    13681 gtccgctggt tgaaatgcat gcgcattacc tggtcgccca ccccagccag aaagtgcagg
    13741 tccagggcaa caccgatgcg cgcggcagcg ccgaatacaa cctggccctg ggccagcgcc
    13801 gttcggaagc cgtcgcccgc atgatgaccc tgttgggcgt caagagcgac cagatcgaag
    13861 ccatcagcta cggtaaagag cgccccaaag ccacaggcag caccgaggcc gactacgccg
    13921 agaaccgtcg tgcagatatt gtctatcagc attaatatct aacacgcaag tcacagtgaa
    13981 gcgccgtggc ggcctcccgc cgcggcgttt ttatttccac ggatcccctc atgaacgtca
    14041 aagccctgtc tcttcgcccg gtactgctcg ccaccgccgt ttcgttcgcg gcgttgtgcg
    14101 ccgcgccggc ccacgcgttc tccgacgacg aagcccggcg cgccatcctc gaattgcgct
    14161 cgcaggtgca gcagttgatc cagcaaaacc agcaggcgcg gttgcaactg gccgaccaga
    14221 tcgaatccct gcagaatgaa gtaaccgata tgcgcggaca aatcgaaaag ctgcggtggg
    14281 aggccgacat cagcaagcgg tcaagccagg accagtccgg cggcgcgacc ccatcggtga
    14341 ccgaccccaa ggagcaggcg gcctatgacc aacccatggg tctgttccgc agcggcaaat
    14401 acaaagaggc cgccacgggc ttcgccgcgt tcatcaagaa tttcccgaac agccaactgg
    14461 cgcccgaggc acagttctac gaaggcagca gccgctatgc cagccgcgac ttcaaggcgt
    14521 ccatcgctga gttgcagagc ctcgttcaca gcaatcccaa ggatgcccgc gcgcccgatg
    14581 cgctgctcat catcgccgcc agccagatcg aactcaacaa cgtggccggc gccaagagca
    14641 atttgcagaa gattgtcaaa gactatcctg acagttcggc tgccgacacg gcgaaaagcc
    14701 gcctgaaact gctcaagtaa tgccaggcga ggcgcgtacc gctccgacac cgccggcgcg
    14761 gtacgcgagg caacggaatc tggtcattct tgccggcgcg ctggcggccc ttctttgcgc
    14821 caacctgctc accggcccgg ctccgatcgg gttcgatgcc ttgcgccatg tcgtcctgaa
    14881 tccgggtgac cactcgacca cggcgatcat cgtgcgccag atccgtctgc cccaggcatt
    14941 gatggcgcta ctgaccggcg ccgcactggg gctatccggc gccgaaatgc agaccatcct
    15001 ggataacccg ctggccagtc cgttcacact gggcgtctcg tcggccgccg ccctgggcgc
    15061 ggcgctggcc atcgtgctga acctgagcgt gccctgggtg cccgaccctt acatcgtggc
    15121 ggtcaacgct ttcgtgttcg cgctggcctg cactgcctta ctccacttgg ccgcccgtca
    15181 cgcacggaca ggcacggccg gcatcatcct gctcgggatc gccatggtgt tcaccttcaa
    15241 cgccctggtt tccctggtgc aattcagtgc cagcgccacg gcgctgcaag acctcacgtt
    15301 ctggatgatg ggcagtctgg cgcgcagcga ctggccccga ctgggggtgg ccgcactggc
    15361 gctttgcgtg atcgcccctt tttctctgcg cagtgcgtgg gccctgaccg ctctgcggtt
    15421 cggcgatgag cgcgccgcca gttttggcgt ggatacgcgc cgcttgcgcc gcgcggcgtt
    15481 ggtgcgcacc agcctcctcg ccgccgtcgc cgtgtcgctg accggagtca tcggtttcat
    15541 tggattgacc gcaccccata tcgcccgacg cctttgggga gaggatcatc gatggtacct
    15601 gcccgccagc gccctgacgg gcagcgccat ccttcttgcc gcggccatcg tgtccaggct
    15661 cggtggtggc ggtgtggtga tccccatagg catcgtcacc accctggtgg gaatcccgtt
    15721 cttctgcgtg gccgtactgc gccggcgccc tgaagcatga acacgtccgg cttcgccacg
    15781 ttgcgactgc gggcattgaa tgtggcattc ggcgccacgc gggtgttcga caacctgacg
    15841 cttccggatc tgagcgcagc cggcatcacc gccttgctcg ggcccaacgg ctgcgggaaa
    15901 tcgacactgc tgaaaagcat cgccggactg gtccccgcga cctgtcacgc cctgacactg
    15961 ggagcctccg acctgaaggg gcttgatgcg gcggcacgca gcaagttcgt tcggtacctg
    16021 ccgcaggccg cattcaagga cgtgcacctg accgtacggg aagccgtcac cgtggctggc
    16081 aagattcgcc gctcggcggc ggaacgccgt cttgacgacg tcgtggacga aaccctgcgt
    16141 gatctgggcc tcgccgcgct gagccaccgc tacctggatg agttgtcagg gggccaaaag
    16201 caattggtcg gcctggccca ggccctgatc caccagccca cgcttttact gctcgacgaa
    16261 ccgctcgcgt cgctcgaccc gaaccatcag atacacgtca tgtcgatgct gacgcggctt
    16321 gcgagcgaac gcgccgtcgc cgtcattgtc gtcctgcacg acctggacat ggcgctgcgc
    16381 ttcgcggacc acatctggct gctgcgcggt ggccggctga tcgccgccgg cattcccgcg
    16441 gacgtgatat cgtccgcgaa catcgcgcaa gcttttggcg tcaccgcacg cgtggaaaac
    16501 gacagcctgg gccagccaca tgttttcgtc gacgatccga tccaactctg agcacatcca
    16561 accatgcccc acgacatcga ccttcctcaa tccgttgccg atggcatgag ctatccattg
    16621 gcctacacgg aatccggatc gggcgcaccg ttgctgctga tccacggatc gctttgcgac
    16681 tatcgctact ggcgctggca gatccccgct tttggtcagg cctaccgcac gctggccccc
    16741 agtctgcgcg gctactggcc cgccgcgttc cgatcggagc gcgcggactt cgacgtgctc
    16801 gtccagaccc gggatctgat cgagttcctg gatcacacca gccccgacgg cactccggtg
    16861 cacgtgctcg gccattcacg cggggcgcgg gtcgcgctgg agctcgcctg tgccgcgcct
    16921 gagaaggtgc gcacgctgac cctggcggac cctgggtttt cgccggtggg ccaggccggg
    16981 cccgtgcctt tccagatcga agccgcgcgc cgcctgagcg acggtgatac cgatggcgcg
    17041 ctcgaggtgt tcatcgacac tgtgagcggc tcggacacct ggcgtcatat ggtgggatgg
    17101 ttcaaggcca tggtgcgcga caacgcggga acgctgctgt cccagatcaa ggaacgtcat
    17161 cacgaattcg atatggcgcg ggcgggcgcc atatcgtgcc ctaccctgct gctcactggg
    17221 tcagacagcc ccgcgcgctt cggacgcgta ctggccagcc tggagcaggt cctgccacac
    17281 gtggaaaaaa ccactattcc gctggcctcg cacggcatga acctggccaa cccacgcgta
    17341 tttaatcagc atgtgctggg ctttctgggc agccattcct gacgcgactt acctggatag
    17401 cgcgccagcc ggcctgacca gacacgtggc ccgcgcgact tcacccgagg cgaaccgcaa
    17461 ctcgaggccg agcgtcgaac ccacgaccag tttgtgcacg ggctgttcca gcatggcgtg
    17521 ataacttccg ggcttgaaag tcaagctgcc ccccgccggc accgggatac tggactccat
    17581 gatcattttg ctcacgccgc cgctttcaac agacttatgc agcatgacca tgccgaacgc
    17641 cgggcttgcc gcactggtca gcaccgccgc atgtgcgcca ttgttgtgaa ccacgaagta
    17701 gccagccgaa ggcgctggcg acggcaatgc ccgtatccag cagccggata cctggagcgt
    17761 cgtcgaactc tgcatagccc ccgcttcgcc atgatcggca tgcggcatgg gggctccccc
    17821 tttcatggac atatggcccg acatgggcat atcgccagac atcgatgtgc caccagcgag
    17881 cgcgacaccg gcgaacaagg cgccgcacac cacgaggaaa ccttccaata cgtgcttctt
    17941 catacccgtc tccactaccc aacaagatac aatgcaggcg ataccgacgt cctggcacag
    18001 acttcgccat gtccgatgca acaagtcatt atgacacgcg ggtccaccag gcgggccgcg
    18061 ccgcacgccg caacacgcca tgaaaacctc gccatcgccc gacaccccgc acatcactgt
    18121 cgccgaattc atccaactac tcgaacggga gcatccgttc gccgcggtgc ttgggctcga
    18181 cgtgctcgag atcgggcacg gtacggcgtt gctgcgcctg cccgaccggg acgatcacaa
    18241 acggctgggc ggcatcgtgg ccggccccat gctgatgggc ttggcggatc tggcccttta
    18301 cgcggccgtc gtcggcgcca ccggaaacac gcatgcggtc accgccagcc tcaccatcaa
    18361 tttcatgcgc aaggcgccag cgggaggaat actggcgcac gcgcgcattc tgaaaaccgg
    18421 cagactgacg gcgggcgaag tgctgcttac ccccactgac ggcggcgatg ccttggccca
    18481 ggtcgtcagc acatggacgc tgcccggccc gcggtcggcg cgaccggccg ccatgcccgc
    18541 gacggcctga cccgccgtga cacaaccgcc gacagcggtt cgcgcgtccg acacgactcc
    18601 cgtcaccgcg acgcgccgtc ccacacccga tcgccggaag ccggccaagc cgtggcgatc
    18661 ggaagtcaag gcgtcgatgt ccctgggctg gcccatcatc ctgacgaacc tggcgcaact
    18721 ggccatactc acgaccgacg tcatactgat cgggcggttg gggccggagc ccctggcggc
    18781 ggggtcgctg tcgatcag
//
LOCUS       DASTNK010000057        18406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1522161, whole genome shotgun sequence.
ACCESSION   DASTNK010000057 DASTNK010000000
VERSION     DASTNK010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 18406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18406
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522161"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1779
                     /locus_tag="VFR20_04330"
     CDS             <1..1779
                     /locus_tag="VFR20_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0229774.1"
                     /translation="GQTIVFIDELHTMVGAGKAEGAMDAGNMLKPALSRGELHCVGAT
                     TLDEYRKYIEKDAALERRFQKVLVNEPDVESTIAILRGLQERYELHHGVEITDPAIVA
                     AAELSNRYITDRFLPDKAIDLIDEAAARIRMEIDSKPEVMDKLDRRIIQLKIEREAVK
                     KESDEASLRRLKVIEDELERLQREYNDYEEIWKSEKAAVQGTQSIKEEIDHIRAEMAD
                     LQRKGQFDKLAELQYGKLPELEGRLKAAEKSESSDAEHPRLLRTQVGAEEIAEVVSRA
                     TGIPVAKMMQGERAKLLGMEDYLHKRVVGQNEAVSLVSDAIRRSRAGLSDSSRPYGSF
                     LFLGPTGVGKTELTKALASFLFDSDDHLVRIDMSEFMEKHSVARLIGAPPGYVGYEEG
                     GYLTEAVRRKPYSVILLDEVEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNTVIVMT
                     SNLGSQHIQSMVGQPYDVVKEVIWDELKAAFRPEFLNRIDEVVVFHGLDKTHIEEIAR
                     IQLNLLARRMAAKDMRLEVSDAAVAQLARAGFDPVFGARPLKRAIQQHVENPLSRLIL
                     EGKFGPNDVVPVDWKNDRFVFSRTLQ"
     gene            complement(1849..2511)
                     /locus_tag="VFR20_04335"
     CDS             complement(1849..2511)
                     /locus_tag="VFR20_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEU0229775.1"
                     /translation="MTPAERPLLTLPENHEKVLLHSCCAPCSGEVMEAMLASGIHYAI
                     YFYNPNIHPVREYEIRKEENVRFAEQHGIEFIDADYDMDNWFERVKGLENEPERGQRC
                     SACFDMRFERTALYAHEHGFDTITSSLGISRWKDMNQINGCGERAAARYPGLVYWTYN
                     WRKGGGSARMIEISKREEFYQQEYCGCVYSLRDTNRHRREQGRDRVKIGIKYYGRDDE
                     QS"
     gene            complement(2522..5326)
                     /gene="glnE"
                     /locus_tag="VFR20_04340"
     CDS             complement(2522..5326)
                     /gene="glnE"
                     /locus_tag="VFR20_04340"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEU0229776.1"
                     /translation="MSDQLILDPALRWSGALRRKTDANPVFGRWLAEAATHPVTRPVI
                     HQWLRELGGSVDQVAASVDIRRILRQLRQRVFFTLMTRDVGGAAPLREVVTAMTVLAD
                     LAVGEAYKSVIAELTARHGIPIDPATGLPQEMLVIGMGKLGGCELNVSSDIDLIMLYG
                     EEGETDGRRRVSHHEFYGMATRRMMPVLSEIDADGQVFRTDLRLRPDGDSGPLAWSLD
                     ALENYLIIQGREWERYAWLKARVMPCKAFADSDTSTQRQQLLTLHRPFVYRKYFDFDA
                     LAALRGLREKIRLDWQRRATARNGVESHHNIKLGEGGIREIEFVVQLTQLIRGGRMPS
                     LQQANLQMALEQQKKAGIIDAGDAERLEAAYVFLRRTEHMLQYREDEQTHLLPGDPSQ
                     RGALAQAMGLTARDFEETLAGHRAFVAQAFRNAFRMVGMGDDNQLATAAPQASRSAAA
                     RTDADARADKTGTHTTGDTQATALDDIEQRAEALLDSHKIRSLPRTSRKRVEALIPGV
                     ILAARKTDTPAQTATRLLDLVEQIAQRSAYLALLAEYPETLARVARIVGASAWAAQFL
                     RHYPLLLDSLIEWRSLLAPADFSAIARQLHDDLSACLLPDGKPDVEQQMNLMRDVQRQ
                     ISFQLLAQDLEGVLSVEKLADQLSALADMLLAETLNRVWPLVQPKGQPPGATPSARFA
                     IIAYGKLGGKEIGYASDLDLVFLYDDAREDAMEIYAKLGRRMTSWLSTMTSSGRLYEI
                     DLRLRPDGDAGLLAVSLDAFTRYQMEQAWDWEHQAITRARFVAGDAELGARFESLRRR
                     ILLLPRDPRKLNEQVRSMRAKISAGHPNSSGDFDLKHDRGGMVDVEFVTQYLVLSHAR
                     EFPTLLDNLGNITLLRLASDVGLIPRDLAAQAADAYRAFRKKQHALRLQGAEKARVPA
                     STLVPERAAVTQLWNTVIGD"
     gene            5427..9152
                     /locus_tag="VFR20_04345"
     CDS             5427..9152
                     /locus_tag="VFR20_04345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HEU0229777.1"
                     /translation="MSLFLRLMGWLALAVYISVAALVLGIRYWVLPHIDQWRPVIAEE
                     MARALGVQVRLGRVQAEWAGLDPSFELTDVDLSSPHGKPILHLPQMRAKLSWRSLLDG
                     VPRFTMLEARGIDLTIRRSRQNNVWIMGQSISLDTASASLSSADLSGLAWLATQRAIR
                     FTDGTVRWVDDSRGAPPLVLSQVTLVFENQGASHEFSLRARLPEGLGSGFELRGVFSS
                     EAVPAAFSLQGLSGRLYARVGGVDTQRWAPWLDLPSGLDLTHVGAQAWATVEEGAVDR
                     VSTVLRLDAAHWLGSPDASVAMGQGSLYVSGAWPTLRHVFSTEPVVPPTPGDTASLAP
                     TAGYPPFDDIQFRLSAKNLAIRSDTLFTQALGFNAVAAQGTVSRDAKRSLSVMLDQAS
                     IDNADMNVAFSGSWHQGGDGAAGIADAHGMFHRASVAAIEHYLPRSLNQDAHDWMRSG
                     LLAGTIHDAGFILKGDLDHFPFGDAPDKGDFHVRGAYSGVVIDYAPARGKRMAWPRLT
                     GMSGEVSLNRVDLRMVATNAVMTPAEGQAPIQLRDLQAQIPNLEGKSVLTVQGDTRGP
                     AMSYLALLRHSPLGGLLGHALDDARADGAWRVPLRMTIPLAHVDDTLVRGAIQFGGGS
                     VTMLAGLPPFTQVTGALDFAENGVSTTGLTARFMDGPVSFSGGFAPSERGLRLSGTMP
                     AKALADYAGVEGMSRLTGHFAYHAAMEREKSGRYALLVDSDLKGLAADLPAPLGKKAA
                     QSLPLHVSWAPYSSAMVLKAVLGDMASLQLVHRAGETKPVFQSGRLVVGRAAGTTPTR
                     GLDIDIARPDVNLDEWRRVYDAFSTPVRGAAVRHTTLFPDLRQLRIESPHVQLAGVRL
                     ENAMISVAPAQAGTPSDWRIDLSSTQSAGTILWREASAGQAGSIVADFQRLALGQADS
                     AKKTETSGSTKEDDTWTADGKLDIPAVSLRAAQFTLYGHPLGKLAVHGINQARGRMWK
                     LDKLRLSSPAFELDGTGEWTLSGPDRGLALQATAKTSDLGAYLDRIGFKNLMRKGQGT
                     LSGTLRWGNLPWAYDKSDLNGQFDIVLRKGSFNYQDSRTARLLKLLSLQSVSRLAKLE
                     WNPGAVLKDGFPYDDVRGTIKMEKGVLKTNNYRVVGPVGTIVLDGSADMNTEKLDLRA
                     LVIPNLDVSGAAIAAGIAINPVIGIGAFLAQWLLRAPLAKAMAVEYRIGGSWDDPVIA
                     EASVKKAPRGTSTPAQGSGKPGAGAPAGRIEPRVVPH"
     gene            complement(9209..10465)
                     /gene="rho"
                     /locus_tag="VFR20_04350"
     CDS             complement(9209..10465)
                     /gene="rho"
                     /locus_tag="VFR20_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEU0229778.1"
                     /translation="MHLTELKALHVSQLLEMATSLEIDNASRLRKQELMFAIMKRRAK
                     QGEQIFGDGVLEVLPDGFGFLRSPDTSYLASTDDIYISPSQIRRFNLHTGDSIEGEVR
                     VPKDGERYFALVKVDKVNSMQPEAIKHRMMFENLTPLHPDKPLMLERDIKSEENVTGR
                     ILDIFAPIGKGQRGLIVASPKSGKTVIMQHMAHAITTNYPDATLIVLLVDERPEEVTE
                     MQRTVRGEVVASTFDEPATRHVQVAEMVIEKAKRLVELKKDVVILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIGTALIETGSRMDEVIYEE
                     FKGTGNSEVHLERRLAEKRVYPAINLNKSGTRREELLIRPDLLQKIWVLRKFIHDMDE
                     IEAMEFILDKIRATKTNAEFFDMMKK"
     gene            complement(10565..10891)
                     /gene="trxA"
                     /locus_tag="VFR20_04355"
     CDS             complement(10565..10891)
                     /gene="trxA"
                     /locus_tag="VFR20_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEU0229779.1"
                     /translation="MSESIKHVSDASFDADVLKSDVPVLVDYWAPWCGPCKMIAPLLE
                     EASKEYQGRVIIAKVNVDENPETAAKFGIRGIPTLMLFKNGKTAATKVGALSKSQLGS
                     FLSESL"
     gene            11128..13089
                     /locus_tag="VFR20_04360"
     CDS             11128..13089
                     /locus_tag="VFR20_04360"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU0229780.1"
                     /translation="MATSHYDEASIRVLKGLEPVRQRPGMYTRTDNPLHVIQEVVDNA
                     ADEALAGYGRHIRVTLHTDASVSVEDDGRGIPVGLHPEEKAPVVELVFTRLHAGGKFD
                     KKAGGAYAFSGGLHGVGVSVTNALAKRLEVRVWRDGGEYAMAFSGGDVAEPLSRVGTA
                     PRRKSGTCVRVWPDTGYFDSASIPVAELSHVLRSKAVLLGGITVSLTVEKTGQTQEWC
                     YTAGLRGYLEESLGDAETIIPLFEGQEYAPDGDEAFAQGEGAQWVIGWTAEGNVVRES
                     YVNLIPTTAGGTHESGLREGLFTAVKAFAELHSLLPKGLRLLPEDVFARASFVLSAKV
                     LDPQFQGQIKERLNSRDAVRLVSGYVKNALDLWLGANVEFGRKLAEIAISQAQARARS
                     SRKVEKRKSSGVAVLPGKLTDCESSDSARTEVFLVEGDSAGGSAKMGRDKEFQAVLPL
                     RGKVLNSWEVERDRLFANNEIHDIAVAIGVDPHGAGDNADLSGLRYGRVCILSDADVD
                     GSHIQVLLLTLFFRHFPRLIEEGHVYVARPPLFRVDVPASGKRPARKLYCLDQGELDA
                     LRDKLVGEGVKADSWSISRFKGLGEMSAEQLWETTMNPDTRRLMPVRYGEQGVDGTRR
                     MFDMLMGKSESGQRRIWLEQKGNLAEVDI"
     gene            13129..15426
                     /gene="parC"
                     /locus_tag="VFR20_04365"
     CDS             13129..15426
                     /gene="parC"
                     /locus_tag="VFR20_04365"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU0229781.1"
                     /translation="MDTTQADLFGQGANDEGLLLADYAEQAYLDYAVSVVRGRALPEV
                     ADGQKPVQRRILYAMRAMGLGHDAKPVKSARVVGDVLGKYHPHGDQAAYDAMVRMAQD
                     FTLRYPLIDGHGNFGSRDGDNAAAMRYTEARLTPFSRVLLDELDEGTVDFLPNYDGSQ
                     KEPQLLPARLPVMLLNGASGIAVGLATEIPSHNLREIAGACVALLKNPKLSDEDLFSM
                     VPGPDFAGGGQIISAPDEIRRIYDGGRGSIKVRARWQFEEMARGQWQWVITELPPGTS
                     AQKILEEIEERTNPKVKAGKKSLTAEQQQTRALLLGLLDTVRDESGKQASVRLVFEPK
                     TSRIDRDEFITTLLAQTSMETSASINLVCIGTDGRPGQRGLRQVLVEWLAFRAQTLTR
                     RTRFRLEKVTDRIHVLEGRMVVYLNVDEVIQTIRESDEPRAALMQRFDLSERQAEDIL
                     EMRLRQLARLEGFKIEKELAAKKDERASLEELLNNPSAFRRMTIREIESDAKLYGDDR
                     RTLIETAERAVLETRVVDEPVTVVISQKGWLRVRQGHGHDPAQFGFKTGDELYRAVEC
                     RSVDDLVAFTDKGRVHTVAVANLPSARGDGQPITSLVDIEPGARIVHMVAGAGDGRYL
                     IGTRDGYGFEATLENMISRQRAGKQFVTLGEGDALLPPLCLRSTDHQLAMLTRKGRFL
                     VIELSELKTLSGGGRGTILMGLDSGDALAQWVCVGPTGITASGVYRNKNTVITLDVKE
                     LAGYVGRRARKGKSLAVKVKDPVLS"
     gene            15473..16507
                     /locus_tag="VFR20_04370"
     CDS             15473..16507
                     /locus_tag="VFR20_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HEU0229782.1"
                     /translation="MSFKVTVQSSHHEFPVAEGQTILDGALDAGIVLPYSCRDGACST
                     CKGKVISGAYDAGHCPAQTLSPEDLAAGYTLFCQARPSSDLVIEAQEIRMASDIQIRK
                     MPSRVMGLDRVADDVVIVSLQLPPADPLHYYAGQYLQFILKDGRRRSYSMANPPTEAN
                     LVELHIRHMPGGAFTDRVFGVDPVPLKVREILRVEGPFGSFFLREDNDKPIIFVASGT
                     GFAPIKAIIERMVASGGTRPATLYWGGRRPADLYMHQKALEWARTLPGFTYVPVVSDA
                     EPEDAWAGRTGFVHRAVMEDFSDLSGHQVYACGAPVMVNAARTDFTHQRGLPEAEFFA
                     DAFISDADIA"
     gene            17040..>18406
                     /locus_tag="VFR20_04375"
     CDS             17040..>18406
                     /locus_tag="VFR20_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0229783.1"
                     /translation="MGQFLERLRYFSRPREPFSEGWGETRKEARQWEDGYRNRWAHDK
                     VVRSTHGVNCTGSCSWKIHVKQGVVTWETQQTDYPRTRPDMPNHEPRGCSRGASYSWY
                     LYSANRLKHPMVRGRLLQHWRDLRQTLGPVQAWTAIMNDPAKHDYKASRGLGGFVRAT
                     WDEALEIAAAANVHTIKKYGPDRVAGFTPIPAMSMVSYSAGTRYLSLLGGTILSFYDW
                     YCDLPAASPQTWGEQTDVPESADWYNASYLIAWGSNVPQTRTPDAHFFVEARYNGTFV
                     VAVSPDYSEVSKLSDIWLHPKQGTDGALAMAMGHVILREFFIDRQVDYFQDYCRRLTD
                     LPMLVRLRQDGGRWVPDRYLRASDLDGALNTPEHAEWKTVGFDEAGRLVAPRGSMGFR
                     WAEEGGSDHGKWNLEQKTADGTDIALQLTEIDRRDAVLDVSFPYFGGVVNEHFPHNDQ
                     RDDVLV"
BASE COUNT         3323 a         5456 c         6106 g         3521 t
ORIGIN      
        1 ggccagacca tcgtcttcat cgatgagctc cacaccatgg tgggcgccgg caaggccgag
       61 ggcgccatgg acgccggcaa catgctcaaa cccgcgctgt cgcgcggcga actgcattgc
      121 gtgggggcca ccacgctcga cgagtaccgc aagtacatcg agaaggacgc ggcgctggag
      181 cggcgcttcc agaaagtgct ggtgaacgaa cccgatgtcg agtcgaccat cgccatactg
      241 cgaggcctgc aggaacgcta cgaactgcac catggcgtcg agatcaccga tccggcgatc
      301 gtggcggcgg ccgagctgtc caaccgctac atcaccgacc ggttcctgcc cgacaaggcg
      361 atcgacctca tcgacgaggc cgccgcgcgc atccgcatgg aaatcgactc caagcccgag
      421 gtcatggaca agctcgatcg gcgcatcatc cagctcaaga tcgagcgcga ggccgtgaag
      481 aaggagtccg acgaagcctc cctgcgccgt ctcaaggtca tcgaggatga gctcgagcgc
      541 ctgcagcgcg agtacaacga ttacgaagag atctggaaat ccgagaaggc cgcggtccaa
      601 ggaacccagt ccatcaaaga ggaaatcgac cacatccgcg ccgaaatggc ggacctgcag
      661 cgcaagggcc agttcgacaa actcgcggag cttcagtacg gcaaattgcc cgaactggaa
      721 ggtcgcctga aggcggcaga aaagagcgaa tccagtgacg ccgagcatcc acggctgctg
      781 cgcacccagg tgggcgccga ggaaatcgcc gaggtggtgt cgcgcgccac gggcattccc
      841 gtggccaaga tgatgcaggg ggagcgcgcg aagctgctgg gcatggaaga ttacctgcac
      901 aagcgggtgg tgggccagaa cgaggcggtc agcctggtgt ccgatgcgat ccggcggtca
      961 cgcgctggtc tgtccgattc gtcgcggccc tacggctcgt tcctgttcct ggggccgacc
     1021 ggtgtgggca agaccgagct gaccaaggcg ctggccagct tcctgttcga ttccgacgac
     1081 catctggtgc gcatcgacat gagcgaattc atggaaaagc attcggtcgc gcggttgatc
     1141 ggcgcacccc ccggctatgt gggctacgag gaaggtggct acctgaccga ggcggtgcgg
     1201 cgcaaaccct atagcgtgat cctgctcgac gaggtcgaga aagcgcaccc cgatgtgttc
     1261 aatgtcttgc ttcaggttct cgacgatggc cggctcaccg acgggcaggg ccgcacggtc
     1321 gatttccgca acacggtgat cgtcatgacg tccaacctgg ggtcgcagca catccagagc
     1381 atggtgggcc agccctacga tgtggtcaag gaagtgatct gggatgaact gaaggcggcc
     1441 ttccgccccg agttcctgaa ccggatcgat gaggtcgtgg tttttcatgg cctggacaag
     1501 acgcacatcg aggaaatcgc ccgcatccag ctcaatctgc tggcgcggcg catggcggcc
     1561 aaggatatgc gcctcgaggt ctcggacgcg gcggtggcac agctcgccag ggcggggttc
     1621 gacccggtct ttggagcgcg gccgctcaag cgcgccatac agcagcacgt tgaaaacccg
     1681 ttgtcgcggc tcattctgga agggaagttc ggccccaacg acgtggtgcc cgtggactgg
     1741 aagaacgata ggttcgtgtt ctcgcgcacg ttgcagtagg cttgcgcacc gctatccgac
     1801 cgtgcgcccc gatacccatg tggtgtcggg gcgtttgtcc acgcgctttc agctctgttc
     1861 gtcgtcgcga ccgtaatact tgatgccgat cttgacgcga tcgcggccct gctcccggcg
     1921 gtggcggttc gtgtcgcgca gcgaatagac gcagccgcag tactcttgtt ggtagaactc
     1981 ttcgcgcttg ctgatttcga tcatgcgggc cgaaccgccg cctttgcgcc agttgtaggt
     2041 ccagtacacc aggccgggat agcgggccgc cgcgcgctcg ccacagccgt tgatctggtt
     2101 catgtctttc cagcgcgaga tacccagtga actggtgatg gtgtcgaacc cgtgttcgtg
     2161 ggcgtacagg gcggtgcgct cgaagcgcat gtcgaagcac gcggagcagc gctggccgcg
     2221 ctcgggctcg ttctccaggc ccttgacgcg ctcgaaccag ttgtccatgt cgtagtcggc
     2281 atcgatgaat tctatgccgt gctgttcggc gaaacggacg ttttcttcct tgcggatttc
     2341 gtattcgcgg accggatgga tgttcggatt gtagaaataa atggcgtagt ggatacccga
     2401 cgccagcatg gcttccatga cttcgcccga gcacggcgcg caacaagagt gcaggagaac
     2461 cttttcatgg ttctcgggca gagtgagaag ggggcgttcg gctggcgtca tgactggtat
     2521 tttagtcccc gatgacggta ttccaaagtt gtgtgacggc cgctcgttcc ggaacgagcg
     2581 tgctggccgg cacccgggct ttttccgcgc cctgcagcct cagggcgtgc tgtttctttc
     2641 ggaacgcccg ataggcatcg gcggcctgcg ccgccagatc gcgggggatg agccccacgt
     2701 cggatgccag gcgcagcagt gtgatgttcc cgaggttgtc cagcagggtg gggaattcgc
     2761 gtgcgtggct caacaccaga tactgtgtga cgaactccac atcgaccatg ccgccccggt
     2821 catgcttgag gtcgaagtct cccgacgaat tggggtggcc ggcgctgatt ttcgcgcgca
     2881 tggaccggac ttgttcattc agcttccgtg ggtcccgtgg cagcagcagg atgcggcgcc
     2941 tcaggctttc gaagcgggcg cccagctcgg cgtcgccggc cacgaagcgt gcccgtgtga
     3001 tggcctggtg ctcccagtcc cacgcctgct ccatctgata tcgggtgaag gcatccaggg
     3061 acaccgccag caggcccgcg tcgccatccg gccgcaatcg caagtcgatt tcgtacaggc
     3121 gcccggacga ggtcatggtg gatagccacg atgtcatgcg gcgcccaagc ttggcgtaga
     3181 tttccatggc gtcttcgcgc gcatcgtcgt agaggaacac cagatcaaga tcggaggcat
     3241 agccgatttc cttgccaccc agtttgccgt acgcgatgat ggcgaaccgc gcggaaggtg
     3301 tcgcgccggg cggttgaccc ttgggttgca ccaggggcca tacccggttg agcgtctcgg
     3361 ccagcagcat gtcagccaga gcggaaagtt gatcagccag cttctcgaca ctgagcacac
     3421 cttccaggtc ctgtgccagc aactggaaac tgatctggcg ctgcacgtcg cgcatcaggt
     3481 tcatctgctg ctcgacatcg ggtttgccgt cggggagcag gcaggccgat aggtcgtcat
     3541 gaagctgtcg ggcgatcgcg gagaagtcgg cgggtgccag caggctgcgc cattcgatga
     3601 gactgtccag caaaagaggg tagtggcgca ggaattgcgc ggcccacgcg ctggcgccga
     3661 cgatgcgcgc cacacgcgcc agggtttcgg gatattcggc cagcagggcc agataggcgc
     3721 tgcgctgcgc gatctgctcg accagatcca acaatcgcgt ggcggtctgg gccggtgtgt
     3781 cggttttccg cgcggcgagg atcacgccgg ggatcagggc ctcgacccgt ttcctgctgg
     3841 ttctgggcag gctgcgtatc ttatggctgt ccagcagcgc ctcggcccgt tgttcgatgt
     3901 cgtcgagggc cgtggcctga gtgtccccag tggtgtgtgt gccggtcttg tcggcccgcg
     3961 catccgcgtc ggtgcgcgcg gcggcgctcc gggatgcctg gggcgcggcg gtggccaact
     4021 gattgtcgtc gcccatgccc accatgcgga aggcgttgcg gaatgcctgc gccacgaagg
     4081 cgcggtgccc ggcaagtgtt tcctcgaaat cacgggcggt caaacccatg gcctgggcca
     4141 gggccccgcg ttgagaggga tcgccaggca ataaatgggt ttgttcgtct tcacggtatt
     4201 gaagcatgtg ttccgtgcgc cgcaggaaca cgtaagccgc ttcaaggcgt tcggcgtcac
     4261 cggcgtcgat gatgccggcc ttcttctgct gctccagcgc catttgcagg ttggcctgct
     4321 gaagagacgg catgcggccg ccccggatga gctgtgtgag ctgaacgacg aattcgattt
     4381 cgcggatacc gccttcaccc agcttgatat tgtggtgcga ttccacgccg ttccgggccg
     4441 tggcgcgacg ttgccagtcc agacggattt tttccctgag accgcgcagg gcggcgagcg
     4501 cgtcgaagtc gaagtacttg cgatagacga aaggccggtg cagcgtcagc aattgctggc
     4561 gttgcgtgga cgtatcgctg tcggcgaacg ccttgcatgg catgacgcgg gccttgagcc
     4621 acgcatagcg ctcccactcg cgcccctgga tgatgaggta gttctccagg gcgtcgaggc
     4681 tccaggccag cggccccgag tcaccgtcgg gtcgcagtcg aaggtcagtg cggaacactt
     4741 gaccatcggc gtcgatttcg gacagcaccg gcatcatgcg gcgcgtggcc atgccataga
     4801 attcgtgatg gctgacgcgg cggcgcccgt cggtttcgcc ttcctcaccg tagagcatga
     4861 tgaggtcgat gtcagaggaa acattcagct cgcacccgcc cagtttcccc atgcctatga
     4921 ccagcatttc ctgaggcagg ccagtggcgg ggtcaatggg gatgccgtgg cgcgcggtca
     4981 gctcggcgat gacgcttttg tacgcctccc ccacggccag atcggccagc accgtcatgg
     5041 cggtgaccac ttcgcgcagc ggggccgccc cgcccacgtc gcgagtcatc agtgtgaaga
     5101 acacgcgttg tcgtagttgc cggagaatgc gccggatgtc gacggacgcg gcgacctgat
     5161 cgacggagcc cccgagttcg cggagccatt ggtggatgac cggccgcgtg accggatgag
     5221 tggcggcttc ggccagccat cgcccgaaga cgggattggc gtcggttttg cggcgcagcg
     5281 cgccggacca gcgcagagcg ggatccagga tgagctgatc ggacatggac tttacaaatt
     5341 atgaaggcga tatagggatt cacggtataa gattcgcaag ataccgcaaa tcctcttgtt
     5401 ttggttggtc tccgttgccg cgtcgattgt ctctcttcct ccgcctgatg ggctggctcg
     5461 cgctggctgt gtatatatcc gttgcggcgc tggtgctggg catccgctat tgggtcttgc
     5521 ctcatatcga ccagtggcgt ccagtgatcg ccgaagagat ggcgcgggcg ctgggagtcc
     5581 aggtgcgtct ggggcgggtc caggccgaat gggcggggtt ggacccgtcc tttgaactca
     5641 ccgacgtcga tctttccagc ccgcatggca agccgatcct gcatctgccg cagatgcgcg
     5701 cgaagctgag ctggcgcagt ctgttagatg gcgtgccgcg attcaccatg ctcgaggcgc
     5761 gtggcatcga cctgacgatt cgtcgctccc gacagaacaa tgtgtggatc atgggacagt
     5821 ccatatcgtt ggatacggcg tccgcgtccc tgtccagcgc cgatctctcg ggcctggcct
     5881 ggttggcgac acaacgggcg atccgcttca ccgacggcac ggtccgatgg gtggatgact
     5941 cgcgcggggc gccgccgctg gtgctgagcc aagtcaccct ggtcttcgag aatcaggggg
     6001 cttcccacga gttctcactc agagcgaggc tccccgaggg gttgggctcg ggattcgaac
     6061 tgcgtggcgt tttctcgtct gaagcggtgc ccgccgcgtt ttcacttcaa gggttgagcg
     6121 ggcgcctgta tgcgcgggtg ggtggcgtgg acacccagcg ctgggcgccc tggctggatc
     6181 tgccatcggg gctggatctg acgcatgtcg gggcgcaggc ctgggccact gtcgaggaag
     6241 gtgcggtcga tcgggtatcg accgtccttc gtctggatgc ggcgcattgg cttggctcgc
     6301 ccgatgccag tgtggcaatg ggacaaggca gcctttatgt ttcgggcgcc tggccgaccc
     6361 tgcggcatgt gttctccacc gagccggttg tgccgccaac ccccggcgac acggcgtcgc
     6421 tggctccgac cgcgggctat ccgccgttcg acgacatcca gttccgcttg tcggcgaaaa
     6481 atctggcgat tcgatccgac acgctgttca cgcaagccct ggggttcaat gccgtagcgg
     6541 cccaaggaac ggtgtcgcgc gatgccaagc ggtcgctgag cgtgatgctc gaccaggcca
     6601 gtatcgataa tgccgacatg aatgtggcgt tttcggggtc atggcaccag ggcggcgatg
     6661 gcgccgcggg gattgccgac gcgcatggca tgttccatcg cgcatctgtc gccgccattg
     6721 aacattatct gccgcgctcg ttgaatcaag acgcccacga ctggatgcgc agtgggttgt
     6781 tggcgggaac catccatgac gcgggattca tcctgaaagg ggatctggac cattttccgt
     6841 tcggcgatgc ccccgacaaa ggcgatttcc atgtgcgtgg cgcatactcc ggcgtcgtca
     6901 tcgactacgc gcctgcccgg ggcaagcgga tggcctggcc caggctgacc ggtatgtcgg
     6961 gcgaggtctc tctgaatcgc gtcgacttgc gcatggtggc gacgaacgcc gtcatgacac
     7021 cggccgaggg ccaggccccc atacagttgc gtgatcttca ggctcagata ccgaatctcg
     7081 aaggcaagtc agtgctcacc gtacagggcg atacccgggg gccggccatg tcctacctgg
     7141 cgctgttgcg ccactctcca ttgggtggcc tgctggggca tgcgctggat gatgcccggg
     7201 ccgacggcgc ttggcgggtt ccgctgcgca tgaccattcc gctcgcccat gtcgacgaca
     7261 cgctggtgcg cggcgccatt caatttggcg gtgggagcgt gacaatgctt gcggggctcc
     7321 cgcctttcac ccaggtgacg ggcgccctgg attttgcgga aaacggtgtc agtaccacag
     7381 ggttgaccgc gcggttcatg gacgggccgg tttcgttctc gggcgggttc gcgccaagcg
     7441 agcgtggcct acggttgtca ggcaccatgc cggccaaggc actggccgat tacgcgggtg
     7501 tggaagggat gtccagactg actggccatt tcgcctatca cgcggcgatg gaacgggaaa
     7561 aatccggccg ttacgcattg ttggtggatt cggatctcaa gggcctggcg gccgatctgc
     7621 cagcgcccct gggcaagaag gcggcgcaat cgctgccgct gcacgtcagc tgggcaccat
     7681 acagttcggc aatggttttg aaggcggtgc ttggcgatat ggcgtcgctg caactggtgc
     7741 atcgcgccgg cgagacgaaa ccggtcttcc agagcggacg gctcgtcgtg gggcgggcag
     7801 ccggaaccac accgacccgg gggcttgata tcgacatcgc gcgcccggac gtgaacctcg
     7861 atgaatggcg gcgtgtctac gatgcgtttt ccactcctgt gcgaggggcg gccgtccggc
     7921 acacgacgct tttccccgat ctgcggcaat tgcgtattga atcgccgcat gttcagcttg
     7981 cgggggtcag gctggaaaac gcgatgattt ccgttgcgcc agcccaggcg gggacaccaa
     8041 gcgactggcg catcgacctg agttccacgc agtcggcggg aacgatactg tggcgggaag
     8101 cctctgccgg tcaggctgga agcattgtgg cggactttca acggctggcg ctcggccagg
     8161 ccgatagcgc gaagaagacc gaaacgtcgg gtagcaccaa ggaagacgat acgtggacgg
     8221 cagacggtaa gctggacatt cccgccgtct cgttgcgggc agcgcaattc accctgtacg
     8281 ggcatccgct ggggaaattg gctgtgcacg gcatcaacca ggcacgggga cggatgtgga
     8341 aactcgacaa gctgcggctg agcagtcccg ccttcgaact cgatggcacg ggtgaatgga
     8401 cactcagtgg gcctgaccgg gggctggcgt tgcaggccac ggcgaaaacg tccgacctgg
     8461 gtgcctatct tgaccggatc ggtttcaaga acctcatgcg caaggggcag ggcacactga
     8521 gcggcaccct gcgttggggc aatctgccat gggcttatga caaatctgac ttgaacgggc
     8581 agttcgacat cgtcttgcgg aaagggtcgt tcaattatca ggattcgcgc accgcgcgct
     8641 tgttgaaact gctgtcgctc cagtccgtca gccgcctggc caagctggaa tggaacccgg
     8701 gtgcggtgct caaggacggt tttccgtacg atgacgtgcg gggcaccatc aagatggaga
     8761 aaggggtctt gaagaccaac aattaccgtg tcgtcggccc cgtcggcacc atcgtcctgg
     8821 atggcagcgc cgacatgaac acggaaaaac tcgacttgcg ggcgctggtg attcccaatc
     8881 tggacgtcag cggcgccgcc atcgccgccg ggatcgccat caaccccgtg ataggcatcg
     8941 gcgccttcct ggcgcaatgg ctgctgcgcg cgccgcttgc caaggccatg gcggtggaat
     9001 accggatcgg tggcagttgg gacgacccgg tcatcgccga ggcgtcagtc aagaaggcgc
     9061 cgagaggcac cagcaccccg gcccagggct ccggaaagcc cggcgcgggc gcaccagcgg
     9121 gtcgcatcga accccgcgtc gtaccacatt aaggcccgct ccccactcgc tcgtgggcgt
     9181 ggaccgcttt actgagagcg aaccaggctc acttcttcat catgtcgaag aattcggcgt
     9241 tggtcttggt ggcgcggatc ttgtcgagaa tgaattccat ggcctcgatt tcatccatgt
     9301 catggatgaa cttgcgcagt acccagatct tctgcagcag gtccggacga atcaggagct
     9361 cctcgcggcg cgtgccagac ttgttcaggt tgatggccgg gtagactcgt ttttcagcca
     9421 ggcgccgttc caggtggact tcggagttgc cggtgccttt gaattcttcg taaatgacct
     9481 catccatgcg gctgccggtt tcgatcagcg cggtgccgat gatggtcagc gaaccgcctt
     9541 cctcgatgtt gcgcgccgca ccgaagaacc gcttggggcg ctgcagcgca ttggcatcga
     9601 cgccgccggt cagcaccttg cccgaggctg gcacgacggt gttgtaggca cgagccagac
     9661 gggtgatgga atccagcagg atgaccacgt ctttcttgag ttccaccagc cgcttggctt
     9721 tttcgatgac catttcggcg acctgcacgt ggcgggtggc cggctcgtcg aaggtggacg
     9781 ccacgacttc gccccgcacg gtgcgctgca tttccgtgac ttcctccgga cgctcatcga
     9841 ccagcagcac gatgagggtg gcgtcgggat agttggtggt gatggcatgc gccatgtgtt
     9901 gcatgatcac cgtcttgcca gatttggggc tggccacgat caggccgcgc tggcccttgc
     9961 cgatgggtgc gaagatgtcc aggatgcggc ctgtgacgtt ttcttcgctc ttgatgtcgc
    10021 gctcgagcat caacggcttg tcgggatgca gcggtgtgag gttttcgaac atcatgcgat
    10081 gcttgatggc ctcgggttgc atgctgttga ccttgtcgac cttgaccagc gcgaaatacc
    10141 gttccccgtc tttggggacg cgcacctcgc cttcgatgga atcgcccgtg tgcaggttga
    10201 aacggcgtat ctgggaaggc gagatgtaga tgtcgtcggt gctggccagg taagacgtgt
    10261 cgggcgaccg caggaagccg aatccgtcgg gcagaacctc gagcacgccg tcgccgaata
    10321 tctgttcgcc ctgcttggct cgccgcttca tgatggcgaa catgagttcc tgtttgcgca
    10381 ggcggcttgc gttgtcgatt tccaggctgg tggccatttc aagcaactgc gagacgtgca
    10441 gcgctttcag ttcggtgaga tgcatgtgtg ggaagagagg gggattttta gagaggtatg
    10501 aacaggatgg cggcgaaccc gaggaagggt tcgcgcgaaa agcgaaaact gcggcggcgg
    10561 gtgattacag cgattcgctg aggaaagagc caagctgcga cttggacaat gcacccacct
    10621 tcgtggcggc ggtcttgccg ttcttgaaaa gcatgagggt ggggataccg cgaatgccga
    10681 acttggccgc tgtctcgggg ttttcgtcaa cgttgacctt ggcgatgata acacgcccct
    10741 ggtattcctt gctggcttct tccagcaacg gcgcgatcat cttgcaggga ccgcaccaag
    10801 gcgcccagta atcgaccagg acggggacgt cggatttcag gacgtcggca tcgaagctgg
    10861 cgtcgcttac atgtttgatg gattcactca tgatggagga ggggagaaaa gagggatgga
    10921 tactgggaaa ctatacgaga ttaacggtcg acagcacagt gtattgtggt aattaggtaa
    10981 ttcgccatgc cgggctgttg ccgatacccg acaagcatac caccgccgaa ccgcccttgc
    11041 atgccttgtc tggcgcgccc gtccgagcgg aaaggcgcgt ggtttgtaaa ataaccgccg
    11101 ttcggcattc gacattctgg aatcgacttg gctacatcac actacgacga agcatccatc
    11161 cgcgttctga aaggactgga acctgtgcgc cagcggccag gcatgtacac acgcaccgac
    11221 aatccgctgc atgtcattca agaggtcgtg gacaacgccg cggacgaggc gctggcgggc
    11281 tacgggcggc acatacgggt cacgctgcat accgacgcca gcgtgtcggt cgaggatgac
    11341 ggccgcggca tcccggtggg gttgcatccc gaggaaaaag cgccggtggt ggaactggtg
    11401 ttcacgcggc tgcacgcggg ggggaagttc gacaagaagg cggggggcgc ctacgcgttt
    11461 tccggtggtc tgcacggggt cggggtgtcg gtcaccaacg cgctcgccaa gcggctcgag
    11521 gtccgcgtct ggcgcgatgg cggtgaatac gccatggcgt tctcgggggg cgatgtcgcc
    11581 gagcccctga gccgcgtggg cacggcgcca cgccgcaaga gcggcacgtg cgtgcgtgtg
    11641 tggccggaca ccgggtattt cgacagcgcc tcgatccccg ttgccgagtt gtcccatgtc
    11701 ttgcgcagca aggcggtgct gctgggcggt atcaccgtgt cgctgaccgt ggaaaaaacc
    11761 ggccagaccc aggaatggtg ctatacggcc gggctgcgcg ggtatctcga ggaatccctg
    11821 ggcgacgcgg aaaccatcat tccgctgttc gaagggcagg agtacgcgcc ggatggcgac
    11881 gaggcgttcg cccagggtga gggcgcgcag tgggtgattg gctggacggc cgaaggcaac
    11941 gtggtgcgtg aatcgtacgt gaacctgatt ccgaccacgg cgggcggcac gcacgagtca
    12001 gggctgcgcg aagggttgtt caccgcggtc aaggcttttg ccgaactgca cagcttgttg
    12061 cccaaggggc tgcggctgtt gcccgaggac gtcttcgcgc gcgccagttt cgtgctgtcg
    12121 gccaaggtgc tggatccgca attccaaggg cagatcaagg agcggctcaa cagtcgcgac
    12181 gcggtcaggc tggtcagcgg ctacgtcaag aacgcgctgg acctctggct gggcgccaat
    12241 gtcgaattcg gcagaaaact ggccgagatc gccatcagcc aggcccaggc acgggcgcgc
    12301 tcctcgagga aggtcgagaa gcgcaagagc tccggcgtgg cggtgttgcc cggcaagctg
    12361 accgattgtg aatcctccga cagcgcgcgc actgaagttt tcctggtgga aggggattcg
    12421 gcgggcggtt cggcgaagat ggggcgtgac aaggaattcc aggcggtatt gcctttgcgg
    12481 ggcaaggtgc tcaactcctg ggaggtcgag cgcgaccgcc tgtttgccaa caatgaaatc
    12541 cacgacatcg ccgtggccat cggcgtggat ccccacggag cgggtgacaa cgccgacctg
    12601 tcgggtctgc gctatggccg cgtctgcatt ctttccgatg ccgacgtgga cggttcgcac
    12661 atccaggtgc tgctgctgac gctctttttc cgccatttcc cccggttgat cgaagaaggg
    12721 catgtttatg tggcgcgtcc cccgctgttc cgggtggatg tgccggcctc gggcaaacgc
    12781 ccggcgcgca aactgtactg cctggatcag ggcgagctcg atgcgttgcg ggacaagctc
    12841 gtgggcgagg gcgtcaaggc cgattcgtgg agcatcagcc gcttcaaggg gctgggcgaa
    12901 atgagcgctg aacagctgtg ggaaaccacc atgaaccccg acacgcggcg gctgatgccc
    12961 gtgcgctatg gcgaacaagg agtcgacggt acgcggcgca tgttcgacat gctgatggga
    13021 aaatccgagt cggggcagcg ccgtatctgg ctggagcaga agggcaatct ggccgaagtg
    13081 gatatctgaa caccgcccgc gcgctgagaa ttttttgaga gttgaccgat ggatactaca
    13141 caagccgact tgtttggcca gggtgccaat gatgaaggcc ttcttctggc tgactatgcg
    13201 gaacaggcgt acctggatta tgccgtctcg gtggtgcggg ggcgggcgtt gcccgaggtg
    13261 gccgatggcc agaagcccgt gcagcgccgc attctctatg ccatgcgggc catggggctg
    13321 gggcacgacg ccaaaccggt caaatccgcc cgggtggtgg gggacgtgct gggcaaatat
    13381 catccgcatg gtgatcaggc ggcctatgac gccatggtgc gcatggcgca ggacttcacg
    13441 ctgcgctatc ccttgataga tgggcatggc aatttcggct cgcgcgacgg cgacaacgcg
    13501 gcggccatgc gctataccga ggcacgcctg acgccgttct cgcgcgtgct gctcgacgag
    13561 ctggatgaag gcacggtcga ttttctgccc aactacgacg gcagccagaa agagccccaa
    13621 ctgctgcccg cccgtctgcc ggtcatgctg ctcaacggcg cgtcgggaat cgccgtcggc
    13681 ctggccaccg aaattccgtc gcacaacctg cgcgagatcg cgggtgcgtg cgtggcgctg
    13741 ctcaagaatc ccaagctgtc ggacgaggat ctgttcagca tggttccggg gcctgatttc
    13801 gcgggcggcg ggcagatcat ttcggctccg gacgagatcc ggcgcatcta tgatggcggt
    13861 cgcgggtcga tcaaggtgcg ggcacgctgg caattcgagg agatggcgcg cggccagtgg
    13921 cagtgggtca tcaccgaact gccgcccggc acctcggctc agaagatcct cgaggaaatc
    13981 gaggagcgca ccaaccccaa ggtcaaggcc ggcaagaaga gtctgaccgc cgagcagcag
    14041 cagacgcggg cgttgctgct ggggctgctg gacaccgtgc gcgacgagtc cggcaagcag
    14101 gcgtcggtgc ggctggtctt cgagcccaag accagccgca tcgaccgcga tgaattcatc
    14161 acgacgctgc tcgcccagac cagcatggag accagcgcgt ccatcaatct ggtctgcatc
    14221 ggcaccgatg gtcgacccgg ccagcgcggc ctgcgccagg tgctggtgga atggctggcg
    14281 ttccgggcac aaaccctgac ccgccgcacc cgcttccgcc tggagaaagt gactgaccgc
    14341 atccacgtgc tcgaggggcg catggtggtc tatctcaacg tcgacgaggt catacagacc
    14401 atacgcgagt cggacgaacc ccgcgcggca ttgatgcaac gcttcgatct gtccgaacgg
    14461 caggccgagg acattctgga gatgcgactg cggcaactgg cccgcctcga aggcttcaaa
    14521 attgaaaagg agctggccgc gaaaaaggat gagcgcgcca gtctcgagga actcttgaac
    14581 aacccgtccg cgtttcgacg catgaccatc cgtgagatcg aatcggatgc caaactgtat
    14641 ggcgacgatc gtcgcacact gatcgagacg gcggagcgcg cggtgttgga aacccgggtg
    14701 gtggatgaac ccgtcacggt cgtgatttcc cagaaagggt ggttgcgcgt gcggcagggg
    14761 catggccacg atcccgcaca gttcggcttc aagacgggag acgagttgta ccgggccgtg
    14821 gaatgccgca gcgtggacga tctcgtggcg ttcaccgata aggggcgtgt ccataccgtg
    14881 gcggtggcga acctgccctc ggcgcgcggt gatggccagc cgatcacgtc cctggtcgat
    14941 atcgaaccag gcgcgcgcat cgtgcacatg gtggccggcg cgggtgacgg gcgttacctg
    15001 atcggcaccc gagacggcta cggcttcgag gccacactcg agaacatgat ctcgcgccag
    15061 cgcgcgggca agcagttcgt gacgctggga gagggcgatg cgctcctgcc gccgttgtgc
    15121 ctgcgttcca ccgaccacca attggccatg ctgacgcgga aggggcggtt tctggtgatc
    15181 gagctctccg aattgaagac gttgtcgggg ggaggccggg gcactattct catggggttg
    15241 gacagtggcg acgccctcgc gcagtgggtg tgtgtcggtc cgaccggtat cacggccagc
    15301 ggcgtctatc ggaacaagaa taccgtcatc acgctcgacg tcaaggaact ggcgggttat
    15361 gtcggccggc gcgcgcgcaa ggggaaatcg ctggccgtca aggtcaagga tcccgtgctg
    15421 tcttaaaata cccgatcaaa tccattttcc caccccacca tcatccattg ccatgagttt
    15481 caaggtcacc gtccagtcca gtcatcacga gttccccgtt gccgaggggc agaccattct
    15541 ggatggggcg ctcgatgcgg gaatcgtcct gccatacagt tgtcgtgatg gggcctgctc
    15601 gacttgcaaa ggcaaggtga tttcgggggc ctatgacgca gggcattgcc cggcccagac
    15661 cctgtcgccg gaagatctgg cggcgggcta caccttgttc tgccaggcgc ggcccagttc
    15721 cgatctggtc atcgaggcgc aggaaatccg catggccagc gacattcaga tccgcaagat
    15781 gccttcgcgg gtgatgggac tggatcgcgt ggcggacgac gtggtcatcg tgtcgctcca
    15841 gttgccgccc gccgacccgc tgcattatta cgccggccag tacctgcagt tcattctcaa
    15901 ggacggccgg cgccgcagtt actccatggc gaatccgccg actgaggcca atctggtcga
    15961 actgcacatc cggcacatgc cgggcggggc gttcaccgac cgcgtgttcg gcgtcgatcc
    16021 ggtaccgctg aaggtgcggg agatcctgcg ggtggaaggg cctttcggct cgttcttcct
    16081 gcgtgaggac aatgacaagc cgatcatttt cgttgccagc ggcacggggt tcgcccctat
    16141 caaggcgatc atcgagcgca tggtggccag cggcggcacc cggcccgcca cgctgtactg
    16201 gggcgggcgc cgtccggccg acctctacat gcaccagaag gcgctggaat gggcgcggac
    16261 cctgccgggg ttcacctatg ttccggtggt atccgatgcc gagccggaag atgcctgggc
    16321 ggggcgcacg ggctttgtcc atcgcgcggt catggaagat ttttccgatc tctccggtca
    16381 ccaggtttat gcctgtggcg caccggtcat ggtcaacgcc gcccgcactg atttcacaca
    16441 ccagcgaggc cttcccgagg ccgaattctt cgccgacgct ttcatttccg acgccgacat
    16501 cgcgtagtct gctgtttggc tggctcgagg gtttgcgacg ccgtgtggcg atgacggcgt
    16561 cgatattcgc ctcggttgtt caatatccat ggcgtgatca gggttataaa aggccgcgca
    16621 ccatgcatga acttaaaatg cgcggaaccg gtgcgtaaga ttcacggtcg atcgcatacc
    16681 gcaaagaaaa tcgttccgca acgattttct gtcactgtct cgacgctata gagggcgacg
    16741 aagcgcccgc tcgaacgaca gggtggcgac cccctcggca ccgcttgctg ttcaacggca
    16801 tcaatgttca atcggcgccc gaaagcgggt agaattgttc agcgtattct gtctattcaa
    16861 agtgtttaca gccgggtatg acacctgtga gtgcgcgtga ttcatgctcc gcgggtttcc
    16921 atcggcttga catcttctgt tgaatcgcaa tcaacaaccc atcctgttcg gcccccatgc
    16981 attccgaacc cctggggcgg gcggtggtca gaggtgaaac tcactctcaa ggtgtggaca
    17041 tgggtcaatt tctcgaacgc ctgcgttact tctcccggcc gcgcgaaccc ttctccgagg
    17101 ggtggggcga gacccgcaaa gaggcgaggc agtgggaaga cggctaccgg aaccgctggg
    17161 cgcatgacaa ggtggtgcgt tccacccacg gcgtcaactg taccggctcg tgcagttgga
    17221 agattcatgt caagcaaggg gtcgtcacct gggagaccca acaaaccgat tacccccgca
    17281 cgcggcccga catgcccaac cacgaacccc gtggttgttc ccgcggcgcc tcgtattcgt
    17341 ggtacctgta tagcgcgaac cgcctgaagc accccatggt gcgcgggcgc ttgctgcagc
    17401 actggcgcga cctgcgccag acgctcgggc cggtgcaggc ctggactgcg atcatgaacg
    17461 acccggcgaa gcacgactac aaggcgtcgc gcggcctggg agggttcgtg cgcgcgacat
    17521 gggacgaggc gcttgaaatc gcggcggcgg ccaatgttca caccatcaag aaatatggtc
    17581 ccgatcgagt cgccggcttc actccgattc ccgcgatgtc catggtgtcg tattcggcgg
    17641 gcacccggta cctgagcctg cttggcggca cgattctttc gttctatgac tggtactgcg
    17701 accttcccgc cgcgagcccg cagacctggg gcgagcaaac cgatgtcccg gagtcggccg
    17761 attggtataa cgccagctac ctgatcgcct gggggtctaa cgttccccag acacgtaccc
    17821 ccgatgcgca cttcttcgtc gaggcccgct acaacggcac gttcgttgtg gcggtcagcc
    17881 cagactactc cgaggtttcc aagctttccg acatttggtt gcaccccaag cagggtacgg
    17941 acggtgcgct ggcgatggcc atggggcatg tcatcctgcg ggaattcttc atcgatcggc
    18001 aggtggacta tttccaggac tattgccgtc gcctgaccga cttgcccatg ttggtccgcc
    18061 tgcgccagga tggcgggcgc tgggtgcctg acagatacct gcgtgccagc gacctcgacg
    18121 gggcgctgaa tacgcccgag cacgcggaat ggaaaacggt gggcttcgat gaggccgggc
    18181 ggctcgtcgc gccccgtggt tctatgggat tccgctgggc cgaagagggt ggctccgacc
    18241 acggcaaatg gaacctggag cagaaaacag cggacggcac cgacattgcg cttcaactga
    18301 ccgagatcga tcgccgtgat gcggtgctgg atgtgtcttt cccgtatttt ggcggggtcg
    18361 tcaacgaaca cttcccgcac aacgaccagc gggacgacgt gctcgt
//
LOCUS       DASTNK010000058        68345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1337776, whole genome shotgun sequence.
ACCESSION   DASTNK010000058 DASTNK010000000
VERSION     DASTNK010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 68345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68345
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337776"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..632
                     /locus_tag="VFR20_04380"
     CDS             99..632
                     /locus_tag="VFR20_04380"
                     /inference="COORDINATES: protein motif:HMM:NF038053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1-like DNA-binding protein"
                     /protein_id="HEU0229784.1"
                     /translation="MATAKKAVKKAAAKKATPAKKAVAKKTVAKKAAVKKVVAKKAVV
                     KKAPAKKVAAKKVVAKKAVAKKPAAKKAVKKVVAKKAAVKKTAAKKAVKKAPAKKAAA
                     KKTVAKKAVAKKAAAKKPAAKKAAAKKPAAKKAAAKKPAAKAAAKASKATAAAPAVKA
                     APNPAASWPFPTGGRPS"
     gene            984..1547
                     /locus_tag="VFR20_04385"
     CDS             984..1547
                     /locus_tag="VFR20_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU0229785.1"
                     /translation="MFGDTFGFLIKTLFSLFGIALIIRAWMYAIRLHPFNPYSQAVLR
                     ITNWLVQPLRHVCPQSHRFDWPSIVGCWLTALVFLSLTWLLGFGMAPSLSVLPGLALA
                     AVLTVLKWTFNLILWATLIQAVLSWVNPMAPVMAVLHTLTAPLLDPIRRMLPNTGGLD
                     FSPLVLLIIAQIAMIMLGHAVYAAFGL"
     gene            complement(1551..2051)
                     /locus_tag="VFR20_04390"
     CDS             complement(1551..2051)
                     /locus_tag="VFR20_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEU0229786.1"
                     /translation="MNSTIFKPRKTVVARLVAATLVAGALSVLAGCANNSASSSVYTY
                     GQAQREQIVRTGTVVALRNVVIQQGQTSGFGGLAGGALGGVAGSSIGRGTGSVLGAVG
                     GAIAGALVGNAIENQTGKTQGLEITVRLDNGETRVIAQAADVPLSVGQRVRLISGDGP
                     TRVVPM"
     gene            complement(2157..3092)
                     /locus_tag="VFR20_04395"
     CDS             complement(2157..3092)
                     /locus_tag="VFR20_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEU0229787.1"
                     /translation="MSEDVLVCGSIAFDTIAVFEGYFKDHILADNIKSLSVSFLVPGM
                     RKEFGGCAGNISYSLKLLGGSPVPVGTVGGDAADYLARFEKLDIDTSLIRILPDMFTP
                     QCFITTDLDNNQIAAFHPGAMGCSADNDLTGRAAAWAIVAPDAKDGMFAHAQRLHAQG
                     IPFIFDLGQAMPLFSGEDLNRMLKLARILTVNDYEASVIEQRTGRTMADIATGLEAVV
                     VTRGAQGATLYTSGTEATIEPAIPDAVVDPTGCGDAHRAGLLYGLVRGWALPDSCRLA
                     NLMGAIKVASQGPQNHAFDRAQIAARLKKTYGLAL"
     gene            complement(3106..3813)
                     /locus_tag="VFR20_04400"
     CDS             complement(3106..3813)
                     /locus_tag="VFR20_04400"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HEU0229788.1"
                     /translation="MSNRSPEDADEADPDEALADDTTPHDKEPFLAQRQSYGVYVEPR
                     TAAPEPEEEPDFLDPLPARYRGPAFYFWGILSVLGVALFLAQCLYVYRAQLAAHLPAV
                     RPLLERACGPLHCRVPYSRDISQITIMNSSLQTRANPLGGQGASPAMQPPEAMVLRVT
                     LRNSLDKPQEWPTLVLDLLDFSGTVVVKKDLPPSLYLPPGVAQRPFAARSEVTAMVPI
                     TMNGVKVSGFQLAKYFS"
     gene            complement(4315..5253)
                     /gene="prmA"
                     /locus_tag="VFR20_04405"
     CDS             complement(4315..5253)
                     /gene="prmA"
                     /locus_tag="VFR20_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU0229789.1"
                     /translation="MRELVLHCLEAQAEALSDALIEAGVLSVSVEDADVDTAHEQPLF
                     GEPGFEPEVQAWRRNRLAALLPDGADPAQIVEQAAALSGLAPQAGWSIREVPDADWVR
                     LTQSQFGPIHVGGRIWIVPSWHQNDPAVPTGVAHPASDADNPIRIQLDPGLAFGTGSH
                     PTTHLCLEWLAEHVRIGDTVLDYGCGSGILAIAAKMLGAGDTHAVDIDEQAVLSTRYN
                     AQVNRVDLDARLPDHTDSAQASVVVANILSNPLKVLAPMLANRVRPGGRLALSGVLER
                     QADEVAQAYAPWLTLSVWRARAGWVCLHGQRTERSR"
     gene            complement(5318..6664)
                     /gene="accC"
                     /locus_tag="VFR20_04410"
     CDS             complement(5318..6664)
                     /gene="accC"
                     /locus_tag="VFR20_04410"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU0229790.1"
                     /translation="MFEKILIANRGEIALRVQRACREMGIKTVVVHSEVDREAKYVRL
                     ADESVCIGPAPARDSYLNMPAIISAAEVTDAEAIHPGYGFLSENADFAERVEKSGFVF
                     IGPKPESIRLMGDKVSAKQAMIEAGVPVVPGSAGALPDDPAEVQRIARDVGYPVIIKA
                     AGGGGGRGMRVVYTEAALHNAVTLTKSEAGIAFNNPEVYLEKFLENPRHIEIQVMADG
                     ERNAVWLGERDCSMQRRHQKVIEESPAPGIERKLIERIGERCAEACRKIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHTVTEMVTGLDLVQMQIRIAAGEKFTLRQRDVELRGHA
                     IECRINAEDPYKFTPSPGKVTNWHVPGGPGVRMDSHVFSGYTVPPNYDSMIAKLITYA
                     PTREQAIARMDIALSEMAVDGVMTNIPLHRDLMHDANFIKGGTSIHYLEHKLAQRS"
     gene            complement(6669..7121)
                     /gene="accB"
                     /locus_tag="VFR20_04415"
     CDS             complement(6669..7121)
                     /gene="accB"
                     /locus_tag="VFR20_04415"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU0229791.1"
                     /translation="MDLRKLKTLIDLVAESGIAELEITEGEGKVKIVKFSPATPAVAY
                     APPPMAAPVMAAPPVAGAPAAPAAPAVPQGHVIKSPMVGTFYRSPNPGASPFIELGQS
                     VKEGEPLCIIEAMKLLNEIETDKAGVVKEILVENGEPVEFGQPLFVIG"
     gene            complement(7223..7657)
                     /gene="aroQ"
                     /locus_tag="VFR20_04420"
     CDS             complement(7223..7657)
                     /gene="aroQ"
                     /locus_tag="VFR20_04420"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEU0229792.1"
                     /translation="MAQRILVLHGPNLNLLGTREPSVYGHQTLAEIDRNLQGLAAREG
                     AGCECFQSNHEGGLVDRIQAAAGDQTDFIIINAAAYTHTSVAVRDALAAVAIRFIEVH
                     LSNVHRREAFRHSSYLSDLAQGVIVGLGAFGYEAAMRYALEA"
     gene            complement(7785..8279)
                     /locus_tag="VFR20_04425"
     CDS             complement(7785..8279)
                     /locus_tag="VFR20_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU0229793.1"
                     /translation="MKRRDFIFQGVAATAFLGAWRPRRALADTGLFGQSFRDVHGTPH
                     AMSSYLGKPLIVNFWATWCPPCVKEMPELDALQKQYPGVRFLGLAVDTANNVREFIQK
                     VPVSYPLLIVGHGGIELVRALGDTTGGIPFTVTFDRQGMIRDRVVGQIKTDSFGAKVH
                     GLVA"
     gene            8374..9747
                     /gene="mpl"
                     /locus_tag="VFR20_04430"
     CDS             8374..9747
                     /gene="mpl"
                     /locus_tag="VFR20_04430"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEU0229794.1"
                     /translation="MHLHILGICGTFMGGLALIARSAGHTVTGCDAGVYPPMSTQLQE
                     QGIHLIEGFDADQLRLRPDLFVVGNVVSRGNPLMEAILDQGLPYTSGPQWLGEHILRG
                     QHVLAVAGTHGKTTTSAMLAWILEHAGLAPNFLIGGIAGDLGVSARYDAQQTVFVIEA
                     DEYDTAFFDKRSKFVHYRPRTAILNNLEYDHADIFPDLAAIETQFHHLVRTIPASGCV
                     IRPVDSPAIDRVLAHGCWTPVDTFGPGGHWQAAGDIDNEFEVRHGAEHASIAWGLSGT
                     HNRMNALAALAAASHAGVSLRTGVEALCRFQGVRRRMELRGTVNQISVYDDFAHHPTA
                     IQTTLEGARNKLAGGRIIAVLEPRSNTMKLGTMASRLPKALEAADRVFCFGALSGKHA
                     LGWDPAQILAPLGARASAYDDLDRMVRAITAESQPGDAIVVMSNGGFGGIHQKLLDAL
                     RIRGSAA"
     gene            9744..10337
                     /locus_tag="VFR20_04435"
     CDS             9744..10337
                     /locus_tag="VFR20_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HEU0229795.1"
                     /translation="MILYLHGFRSSPQSFKATLLAQAMRARGLEDAWLCPQLPASPAQ
                     AMALADRLIRKALPGGIEQTRAELVIIGSSLGGYYATCLAEHWQCRAALLNPVVYAAR
                     DLATQVGEHTLYHGGGPFTFLPEYIDELAEMAVGRPTHPERYYLLASTGDEVLDWREM
                     ADWFQGGRQHVINGGDHGLSDFPAHLPEVLEFALTGT"
     gene            10397..12283
                     /locus_tag="VFR20_04440"
     CDS             10397..12283
                     /locus_tag="VFR20_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HEU0229796.1"
                     /translation="MHVLYEDNGGFKAGRIFSEGESTMQVEAESGKRSKIKNASVLFR
                     FDQPAPAALLEQAQALADTLETDFLWECAPQEEFLAADFAEDYFGHPPSAVEKAALIF
                     ALHNAPAYFHRRGKGGYRPAPPDILAAALAAIDKKRKQAEQQQEWTDALVAHHMPAPI
                     AEAATDLLIRPDKNSMQWKALDAAVKQLATTPEKLLLEAGAWPHPLALHRARFLAANF
                     PHGTTLPPVEVGTPGGDLPLSDVIAYSVDDISTTEVDDAISVTMQGDDRALIGIHIAV
                     PALAVTRDNPLDRLARERMSTVYAPGQKIPMLPDALIEAFSLDEGKPRPALSLYVQAD
                     LGSGEILSSETRLERIQVRENLRHNHLDELITEEALDDPGADLPYGEWLRPLWRFARH
                     LATQREQVRGKPENNNRIDYQFYLEGPADDPDARVILAPRRRNAPLDRLVAEYMILTN
                     NLWGGLLARHGVPGIYRSQQAGRVRMSTQALPHEAIGVPQYAWTTSPLRRYVDLINQG
                     QLLATVEHGVSARLVAPFKPKDADLFAIIGAFDSQYAIWGDYQTAMERYWCMRWLQQQ
                     GLRRVRATVIRDDLARLDCAPLVTRVSALPALERGQAITLDISGYDELNLEMDCRYVE
                     TVSD"
     gene            12397..13227
                     /locus_tag="VFR20_04445"
     CDS             12397..13227
                     /locus_tag="VFR20_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU0229797.1"
                     /translation="MRVALPISLALHAALLAVRFSSPPPPAPEASTLEVVLVNARTKA
                     APIKPRLLAQNTLNGGGPATSGYSASPLPRTTEDQTANQVVLAALRQRQARLEKEQKQ
                     LFTLLVDRQKTLLAQIERDVPDDSTDAGEDERTQEALVVNAQISALKAAIQRYDARPR
                     QRFVAPSTQAVPYAKYVEAWRKKIELLGTEHYPEQARGKVYGKLRMTVYIKKNGELLR
                     VEIDQPSSHPILNSAAKRIVQLAAPFAPFPPALAKNTDVLAITRTWHFVNSELTTSPQ
                     "
     gene            13224..14084
                     /gene="aroE"
                     /locus_tag="VFR20_04450"
     CDS             13224..14084
                     /gene="aroE"
                     /locus_tag="VFR20_04450"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU0229798.1"
                     /translation="MTEPTPPRRCAVIGNPIAHSRSPFIHLRFAEQTGLDLRYDRILA
                     EADDFEAVATDFFHGGGIGLNVTVPFKEKAFTLTHRRLDDSARVAQAVNTLYVREGVL
                     HGANTDGTGLLADLERLGCTLSGKRVLLVGAGGAARGVAAPLLASGCALLHVVNRHAE
                     RAHILRQHIHEISPATAGRLSSGGLRDADGDWDLIINATSGSLQGQAPDIPAARYAAG
                     ALAYDLVYSARPTPFMIQASRLGADQVSDGLGMLVSQAAASFAIWHGRYPDALPVLAA
                     LRQALDAETR"
     gene            14081..14818
                     /gene="mtgA"
                     /locus_tag="VFR20_04455"
     CDS             14081..14818
                     /gene="mtgA"
                     /locus_tag="VFR20_04455"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEU0229799.1"
                     /translation="MNARRKRRSSRHGLVRAVVLIGISAVLLYLLGLFLMVVWYNFRN
                     PDTSAFMRETLIELRATNPHATLVHEWVPYAGISINLKRAVIASEDSTFVENDGVNWD
                     AIREAWLYNQRQKRRGSDKRRGGSTITQQLAKNLFLSSSRTYLRKGQELILAYMINAI
                     MSKRRILELYLNLAQWGDNLFGAQAAARRYYHIDASRLSQRQAAQLAVMLPDPDYFGD
                     HRSSSYLQRRTNVIEQRMRLVDIPGVH"
     gene            14911..16086
                     /gene="lptG"
                     /locus_tag="VFR20_04460"
     CDS             14911..16086
                     /gene="lptG"
                     /locus_tag="VFR20_04460"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEU0229800.1"
                     /translation="MRTARRYLALEIYRTCAVVLLALVGLFTFFALIDDLNNVSATFS
                     LLNLFYLQALAIPTRLYDLLPIGLLIGAILALAGLAQRNELVILRVSGVSGLKLLTML
                     WAITIPLVLGALFLSEIVTPAAEIKSSEASLMMLGRSNGGRLDSGYWFKESSPETGGT
                     RIINIKKLKANGGVAGVVVYEFNERHQLVRYSQATDGRFGDGKLILDHVTENKIDGNA
                     ISALANAKQPTTPVTTVDTIAERTLATTLTPDRLIARILTPERMSIIDLLDYINYLKA
                     NHLQTDRQVVALWRKVAYPFTLLVMMTIAAPIGFMQTRRGGVGSKVFIGIILGVLFFM
                     LNQLALNVGMLSKWPPWITAIVPNVVVLLLALGALLLMENQHHVRAFRQRNQAQPAA"
     gene            16089..16979
                     /locus_tag="VFR20_04465"
     CDS             16089..16979
                     /locus_tag="VFR20_04465"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEU0229801.1"
                     /translation="MARSESSVAPRHSDHSPDAGTHPPTPIWIIGDLQGCCAALSELL
                     EHPDIATDPRARFWFAGDLVNRGPDSLGTLRSVMALGERATAVLGNHDLHLLAAAAGI
                     RSPGRSDTLDDILNAPDAGDLIDWLRHRPLAHYEHHHLLVHAGVLAGWSVTKTLELAR
                     EVEMSLRSADWRKHLAHMYGNKPVRWKDSFSGHKRQRAIINALTRMRMCHPNGDMEFK
                     HKRGPEPGQELMPWYDVPGRVAHDVTVVFGHWSALGLLVRPDVICLDTGCVWGGKLTA
                     MRMHDHHLVQVACAQFRKPG"
     gene            complement(16984..17730)
                     /locus_tag="VFR20_04470"
     CDS             complement(16984..17730)
                     /locus_tag="VFR20_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU0229802.1"
                     /translation="MNVLRFVARLCVVLVWILFGLLCVSILFPLVPRVRRAVTRWWSA
                     ALMALCGVAVRIAGQPVLDRPVLWVANHVSWVDIFVLNSVRCVSFVSKSEVRRWPVIG
                     WLVAGAGTVFLDRGRRNAIRAVAEQMGRRFGQGDAVGLFPEGTSSSGFDVAPFHASLF
                     EPAIQAGADVQPVALRFIHKGRRSDHLSFVGDQTLAHNVWLLLSTTGARVDAEFLPVI
                     AESDCRGLGRAGVAARAHKVVRRAVIADTF"
     gene            complement(17804..18214)
                     /gene="ruvX"
                     /locus_tag="VFR20_04475"
     CDS             complement(17804..18214)
                     /gene="ruvX"
                     /locus_tag="VFR20_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU0229803.1"
                     /translation="MTEETLLAFDYGLRKIGVAIGNTLTRQARPLETLVATTREQRFQ
                     AIARLVREWEPDRAVVGLPLTLDGGEQYASLRCRRFANQLHGRFGLDVDLVDERNTSM
                     EAQRMLGSSGPEDDAMAAAIILQRYFDALEASSC"
     gene            complement(18207..18800)
                     /locus_tag="VFR20_04480"
     CDS             complement(18207..18800)
                     /locus_tag="VFR20_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEU0229804.1"
                     /translation="MEPSTPRSGNGSSVDLSRQFLVAMPGKVGGSLANSVIYMCEHTE
                     QGALGLVINRPTDLTLGNLLERIELDLSLEIGPIRDDPVFFGGPVQTDRGFVLHTPKG
                     NYSSSIELGDVALTTSRDVLQEIAQGHGPGHVLVTLGYAGWGAGQLESEISQNAWLNT
                     RASMEILFETPPAERYDAALGLLGIDATMLTGDAGHD"
     gene            complement(18845..19009)
                     /locus_tag="VFR20_04485"
     CDS             complement(18845..19009)
                     /locus_tag="VFR20_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015389172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEU0229805.1"
                     /translation="MRTWMCLICGWIYDEAAGLPEEGIAPGTRWEDVPPNWVCPECGA
                     RKEDFELIEV"
     gene            19085..19888
                     /locus_tag="VFR20_04490"
     CDS             19085..19888
                     /locus_tag="VFR20_04490"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU0229806.1"
                     /translation="MPISSPPLALIFGPFDPSGSSNLPADAVTCALLGGHALGVLTGI
                     HVQDTAGIEAIQPVAPDLIDDQARCLLEDMAVGAMKAGPLYTVESVSAVAQIVADYNP
                     VPLVLHLGALPDEETLADGLDAEELVGAVCELLLPQADLVIADHNLITLWAEHGFLNS
                     RDSGSAAEILLEYQAKWALITGAPLRPGHRCYQLVGADNQTHHWPWAPPPERVTGADG
                     PLGCAVALELARGQEVPLAVETAIRQTAAMAEQSFQPGMGHRLINRSHP"
     gene            19885..20538
                     /gene="thiE"
                     /locus_tag="VFR20_04495"
     CDS             19885..20538
                     /gene="thiE"
                     /locus_tag="VFR20_04495"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU0229807.1"
                     /translation="MTPALKFPRGLYGITPEWDDAKRLMRAIEAAATGGMTALQWRRK
                     TIDQADALPQARAVAALCRELGVCFIVNDDWRFAAAIDADGVHIGREDGPLSDARIAL
                     GPDKILGCSCYNEPALAEQALRAGADYVAFGAMYPSMVKPGAVRATLDHIHAGRELAT
                     QLADAGARAAVIAIGGITPANAAPIVAAGADSIAVISGLFEAPDIRAAAQACSALFM"
     gene            20627..21904
                     /gene="hemL"
                     /locus_tag="VFR20_04500"
     CDS             20627..21904
                     /gene="hemL"
                     /locus_tag="VFR20_04500"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEU0229808.1"
                     /translation="MSRNPELFDRACQTIPGGVNSPVRAFRSVGGAPRFIQRAQGPYI
                     WDVDGARYIDYIGSWGTAILGHAHPEVVRAVQDAAVNGLSFGTPTEAEIEIAETIAAR
                     IPSIEKVRLVSSGTEATMTAIRLARGYTGRSKIIKFEGCYHGHADSLLVKAGSGMLTF
                     GNPTSAGVPPEFIRHTLVLDYNRLDSVKEAFRQHGADIACVIIEPMAGNMNLVKPVPG
                     FLEGLRQLCSEHGALLIFDEVMTGFRVGPQGVQGLTGVQPDLTTLAKIIGGGMPVGAF
                     GGRADIMEHIAPIGSVYQAGTLSGNPVAVAAGITTLRLLEAPGFYEDLSVGTARLTNG
                     LAERAKAAGVSFCADSVGGMFGLYFSSNIPTTFDEVSHCNIERFNKFFHGMLTRGVHF
                     APSAFEAGFMSAAHGDAEINATLDAAEAVFAKL"
     gene            complement(22015..22428)
                     /locus_tag="VFR20_04505"
     CDS             complement(22015..22428)
                     /locus_tag="VFR20_04505"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU0229809.1"
                     /translation="MEFLQPPDWTPPKGYANGVMTELMVGGRLLFVGGQIGWNGQQVF
                     ETDDFSGQVAQALRNIVDVLAQGGAEPRHIVRMTWYVRDRDEYVASLREMGRHYREII
                     GRHFPAMTAVEVAALIEDRARVEIEVTAVVPPAPA"
     gene            complement(22482..24125)
                     /locus_tag="VFR20_04510"
     CDS             complement(22482..24125)
                     /locus_tag="VFR20_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0229810.1"
                     /translation="MERSAHIDTFARDHLPPLDQWPEMLIAGNPDVAYPARLNCAVSL
                     VDDVAARAGADRVALHWRENGEQRAMTYGELAALTNRIAHVLTEDMGLVPGNRILLRG
                     PNNPMMAASWLAAVKAGLVTVPSMPLLRSGELKKLVDKARISAALCDARLADELEHCR
                     DSSHEHYCADLAHVRYFNGDGPDSLEALAAAKPAGFTACDTAVDDVCLIAFTSGTTGK
                     PKGCMHFHHDVLAMCDTFSRHILKMMPNDVVCGTPPLAFTFGLGGLLCFPLRVGASVV
                     LVEKLTPAELLATVQDFGVTMTFTAPTFYRQMAALAPGHDLSTLRASVSAGEALPDDT
                     RHLWRDATGVEMIDGIGGTELIHVYVSSANERVRAGAIGRAVPGYVVRVVDQNLEEVP
                     RGTIGRLAVKGPTGCRYLDDDRQRAFVQQGWNLPGDTFIMDEDGYLFYQARNDDMIVS
                     AGYNIAGPEVEAALLTHEAVAECGVVGVADPARGNIVKAFVVLKPGCLGDSAMVKTLQ
                     DHVKATIAPYKYPRAVVFVDSLPRTGTGKLQRYALRNLS"
     gene            complement(24169..25347)
                     /locus_tag="VFR20_04515"
     CDS             complement(24169..25347)
                     /locus_tag="VFR20_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0229811.1"
                     /translation="MDDTTWLEWPFFEGRHRELATSLAAWCASDLPQGDHADTDATCR
                     RLVRALGAGGWLRYVVPHGSAGKWGGRWPEIDSRAVCLLRETLAFHDGLADFACAMQG
                     LGSGAISLGGSDALRERYLPEVAAGNAIAAFALSEPDAGSDVAAMSCRAEAVEGGYVL
                     NGEKTWISNGGIADFYCVFARTGEAPGARGISAFVVDAGTPGLTIPRRIDVMAPHPLA
                     TLEFKECRVPASQRLGEAGHGFKLAMMTLDIFRASVAAAALGFARRALHEALRHSVSR
                     RMFGQRLADLQLTQSALGDMATEIDAAALLTYRAAWLRDVKKARTTREAAMAKMAATE
                     SAQRVIDRAVQMFGARGVVSDAPVERLYREIRALRIYEGATEVQKLIIAREMLKVVGQ
                     "
     gene            complement(25348..26208)
                     /locus_tag="VFR20_04520"
     CDS             complement(25348..26208)
                     /locus_tag="VFR20_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEU0229812.1"
                     /translation="MEDTRQVAHNMAKHKRAMAGYQARHFLWSASDDGKVATITLNRP
                     ERKNPLTFDSYAELRDLFRALVYATDIKTVVVAGAGGNFCSGGDVHEIIGPLTKMNMP
                     QLLDFTRMTGDLVKAMRACPQPIVAAVDGICAGAGAMVALAADFRFGTAAARTAFLFT
                     RVGLAGADMGACSLLPRTIGQGRASELLYTGRSMSADEGLAWGFFNSLHEPDELLRAA
                     QTMARQIADGPTFAHAMTKTLLREEWAMSLDDAIEAEAQAQAICMQTRDFLRAYEAFS
                     AKRKPVFEGD"
     gene            complement(26226..27008)
                     /locus_tag="VFR20_04525"
     CDS             complement(26226..27008)
                     /locus_tag="VFR20_04525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0229813.1"
                     /translation="MSNQPAFRHAVVTGGARGIGLAVARRLLGRGLCVTLLGRGAAPL
                     DAAVRELDAPGRVARVQADVVHHDQVGGAMDAARREFGPVDVLVNNAGQAISERFDRM
                     TDDQWRDMIAVNLTGTFHCIQSVLPDMLAGQRGRIVNVASTAGLVGYAYVSAYCAAKH
                     GVVGLTRSLARELAGKGVTVNAVCPGYTETDLVRDAVRNIIAKTGMTEQQAREKLAER
                     NPQRRLVQPDEVADTVAWLCGPGAASVNGQAIAVDGGEVATS"
     gene            complement(27005..29359)
                     /locus_tag="VFR20_04530"
     CDS             complement(27005..29359)
                     /locus_tag="VFR20_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HEU0229814.1"
                     /translation="MKIVCIGGGPAGLYFGLLMKLSDARHEVVVIERNRPYDTFGWGV
                     VFSDATLENLTEADPVSAGRIISAFNRWDDIDIHYRGGTIRSGGHGFIGIGRKKLLNV
                     LQARCEELGVSLVFETDVSDDQEVALRYGADLVIASDGLNSRVRKRYADTYKPDVDTR
                     QCRFVWLGTEQTFDAFTFAFVQTEFGWFQAHAYQFEPGLSTFIVETRDETWRAAGLET
                     MSQEEGIAYCERLFAPWLGGHRLVCNATHLRGSAIWIRFPRVICDTWVHRQVLPDGRE
                     VPVVLMGDAAHTAHFSIGSGTKLALEDAIELHRCLTDQGSDLRAGLDHYQDVRSVEVL
                     KIQNAARNSTEWFENVARYANFDPEQFAYSLLTRSQRISHENLRLRDAEWLEGYERWM
                     LARQNDGLQPQAPPSAHPVPPMHLPYTVRGITLKNRIMVSPMAMYSCVDGVPGDFHLV
                     HLGARAMGGAGLVMVEMTCVSPDARITPACPGMWNDTQQEAFARIVRFVHENSTARIG
                     MQLGHAGRKGSTRVSWEGTDQPLPSGNWPLVSASPIPYIPGVSQVPAGMTHEQMRDVL
                     ERFVAAARRAAKAGFDWLELHCAHGYLLSSFISPLTNLRVDAYGGSLENRLRYPLEVF
                     QAVREVWPAHLPMSVRISAHDWVEGGITADDAVEIARRFKAAGADMVDCSSGQVSKEE
                     KPVYGRMFQTPFADRVRNEANVPTIAVGAIFEADHVNSIIASGRADLCALARPHLADA
                     AWTLRESARIGYADVAWPKQYFPAKRQLETNFERAAAYAQQIGK"
     gene            29517..30020
                     /locus_tag="VFR20_04535"
     CDS             29517..30020
                     /locus_tag="VFR20_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0229815.1"
                     /translation="MNDFPAASIEQAIPDLESRTVPEDHQDLRLWLRLLTCSNLIEGT
                     IRSRLRTEFSTTLPRFDLMAQLLRAPDGMKMSDLSQHMMVTNGNITGITDQLEKEGLV
                     ERVKVATDRRSSLIRLTTKGKRTFRKMAQAHEAWIQSLFSGLSDTSRKALYDALGELK
                     LVSRTRR"
     gene            30187..31413
                     /gene="argJ"
                     /locus_tag="VFR20_04540"
     CDS             30187..31413
                     /gene="argJ"
                     /locus_tag="VFR20_04540"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEU0229816.1"
                     /translation="MAVNLHIPKESEIHAVPGIEIGIAEAGIRKAGRRDLTVFRLAPG
                     TNVAGVFTRNRFRAAPVQVCEKHLASGAAIRALVINTGNANAGTGEPGLSAANQTCAS
                     LAGLLGLEPGQVLPFSTGVILEPLPVDRLVAALPAAAASLTASNWFAAAHSIMTTDTQ
                     PKIVSRRVEVGGKTITITGVSKGAGMIRPNMATMLGFLATDAGLPTALLSKAAKAIAD
                     RSFNRITVDGDTSTNDSFIIMATGQSGLNIAGESDPAYPAVFEALASAATELAQKIIR
                     DAEGATKFMTIRVEQAANQEEALKVAYAVAQSPLVKTAFFASDPNLGRILCAIGYAGI
                     DDLDVTRLNLWLGDVLVASAGGRHPGYREEDGQRVMKEAEIVVRIALGRGDTAETVYT
                     CDFSHDYVSINADYRS"
     gene            31591..31902
                     /gene="rplU"
                     /locus_tag="VFR20_04545"
     CDS             31591..31902
                     /gene="rplU"
                     /locus_tag="VFR20_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU0229817.1"
                     /translation="MYAVIKTGGKQYRVAAGQKLKIEQIPADIGQEITLDQVLSVGEG
                     DQLQIGTPFVANAVVKATVLAQGRHDKVRIFKMRRRKHYQKRQGHRQNYTEVRIDAVA
                     A"
     gene            31942..32202
                     /gene="rpmA"
                     /locus_tag="VFR20_04550"
     CDS             31942..32202
                     /gene="rpmA"
                     /locus_tag="VFR20_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU0229818.1"
                     /translation="MAHKKGGGSTRNGRESESKRLGVKTYGGQAISAGAIIVRQRGTQ
                     FHPGTNVGIGKDHTLYSLINGTVKFSVTGALNKRTVSVLAGE"
     gene            32356..33486
                     /gene="obgE"
                     /locus_tag="VFR20_04555"
     CDS             32356..33486
                     /gene="obgE"
                     /locus_tag="VFR20_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU0229819.1"
                     /translation="MKFVDEATIEVVAGKGGNGVSSFRREKFIPKGGPDGGDGGRGGS
                     IYAVADRNINTLIDYRYARLHRAKNGENGRGSDQYGAGAPDIVLRVPVGTIVYDADTG
                     QQLFDLNHHEDKVVLVAGGEGGWGNIHFKSSTNRAPRQWSPGKEGEQRKLRLELKVLA
                     DVGLLGMPNAGKSTLITKISNARPKIADYPFTTLHPNLGVVRTTASRSFVVADIPGLI
                     KGASEGAGLGHLFLRHLSRTRVLLHLVDVAPLDPEVDPVEHAAAEARAIVEELRLYDT
                     ALYEKPRWLVLNKTDMVDDPAGLEERLCKALDWTGPVFRISALNGEGTQELTWELQDW
                     LDAEREKDFLAQAQAQADYADPDPRFDQTRGDASITGAPHDQ"
     gene            33559..34716
                     /gene="proB"
                     /locus_tag="VFR20_04560"
     CDS             33559..34716
                     /gene="proB"
                     /locus_tag="VFR20_04560"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU0229820.1"
                     /translation="MPSDSPISSLVADARRLVVKVGSSLVTNEGKGIDRAAVASWASQ
                     IADLHRQGRQMVLVSSGAIAEGMVRLGWPQRPHTMHELQAAAAVGQMGLVQAYEAAFA
                     THAIRAAQILLTHEDLADRRRYLNARTTLYTLLGLGVVPIVNENDTVVTDEIRLGDND
                     TLGALVTNLIEADALIILTDQDGLYNSDPRKNPDARLIQTGQAGDPALETMAGGAGSG
                     IGTGGMLTKVLAARRAANSGGHTIIASGREPDVLLRLAAGEPIGTELRALLPVRSARK
                     RWLANHLRLRGRVTLDAGAVQALTQNHKSLLAIGVIKVEGEFERGDVVACVDQNGVEC
                     GRGLINYSSGDTRRIMRQPSRRIPDILGSMSDQELMHRDNMVFVRPGRRPA"
     gene            complement(34777..35805)
                     /locus_tag="VFR20_04565"
     CDS             complement(34777..35805)
                     /locus_tag="VFR20_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0229821.1"
                     /translation="MEKSTNTSLDDMALFVEVARRKNFSRAAESSGVPVSTLSRRISG
                     LERCIGVKLLHRSTRRVELTEAGSVYFERCRNIIDKARIAHEQLVETARRPQGRLRVA
                     VPTSLAVMSLSEILRDFCTQYPEIECEFDLSMRQVDLLTEPYDMALRLGEQPDSGVVS
                     RHIDDLQLGLYAARDYLARRGVPLEPADLEHHVCLRRSAGKTDAIWRLRCQGRQELVP
                     VSGAVAANDAGMLRRLCLQGMGIAQLTEYLSRCDDGMQRLVRVLPGWDFGPMPLFALF
                     PSRLVPAKVRVFIDFLQDRLPALRAAREASLRSDGPVVLGAADGAGVPPGDGAGVLPG
                     DRGSVLPG"
     gene            36047..36421
                     /locus_tag="VFR20_04570"
     CDS             36047..36421
                     /locus_tag="VFR20_04570"
                     /inference="COORDINATES: protein motif:HMM:NF025045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HEU0229822.1"
                     /translation="MKAILGTAMISLSLLAAGAQAAVSDVAPTRAQVVAELQQARSAG
                     LLSEHEADPYPVPVVAASHKSRAEVLAELAQARSAGQLSLHEADPYPSPAADADPVAS
                     RAQVLAELAQYQSAHPREFIEH"
     gene            complement(36497..37444)
                     /locus_tag="VFR20_04575"
     CDS             complement(36497..37444)
                     /locus_tag="VFR20_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0229823.1"
                     /translation="MDTSLDDIALFVEVAKRRNFSRASESSGVPVSTLSRRINQLEHN
                     IGVKLFNRSTRRVELTEAGGVYFERCQKIVDEARIAHEQLVDVAQQPKGRLRVSLPAS
                     FAVITLAGFLGEFCTLYPDIECEFDLGIHPVDLLADPFDLVLRFGQQPDSGVVSRHLV
                     SVSLGLFASRRYIANHGMPVVPAELAHHQCLRATANKEDSNWELHGGDRVETVAVAGR
                     IMANNVGMLQRMCQQGLGIVPLSCRMSRGQEGEEPLVRVLPEWEFRPMPLLALFPSRL
                     MPAKTRVFIEFLQARLVELQAATKAQGLLKQTARSGVVA"
     gene            complement(37463..37951)
                     /locus_tag="VFR20_04580"
     CDS             complement(37463..37951)
                     /locus_tag="VFR20_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEU0229824.1"
                     /translation="MRVQRQPVSTLDKLDRHILDLLQNNGRMSMTELSEAVGLTVTPC
                     AERVKRLERDGIITGYHARVLPGALDAGLLVFVEISMSSKSDRTFDDFRRAILCIPEV
                     QECHLVSGDFDYLVKARIKEIGQYRHLLGDILLRLPGVTQTKSCVVMEEVKETLFIAA
                     QK"
     gene            38185..39435
                     /locus_tag="VFR20_04585"
     CDS             38185..39435
                     /locus_tag="VFR20_04585"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEU0229825.1"
                     /translation="MKVLVLGAGVIGVTSAYFLARQGHDVTVIDRESAAAMETSYGNA
                     GQLSFGYSSPWAAPGVPLKALKWMFAEHPPLTIRPDGSLFQLKWLFQMWRNCTAARYA
                     INKERMVRISTYSRQCLEQLRADTGIEFEGRSRGTLQLFRTQHQLDGISQDLAVLAEA
                     GVRYDVLSREDLVKAEPALANSLHKLAGGLRTPDDETGDCHLFTTRLAALAREAGVDF
                     RYGVDIRKLRFGAGAIEGVECSTGTMTADAYVVALGSYSRLMLDGNLSLPVYPLKGYS
                     ITVPITNETAAPVSTLLDETYKIAVTRFDNRIRVGGMAEIVGYSKALNPKRQKTLEMV
                     LNDLFPGSHAPGSAHFWTGLRPKTPDSTPIVGKTAYRNLFLNTGHGTLGWTMSCGTGQ
                     LLADIVSGHETAIRSDDLNLSRYR"
     gene            complement(39426..40148)
                     /locus_tag="VFR20_04590"
     CDS             complement(39426..40148)
                     /locus_tag="VFR20_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0229826.1"
                     /translation="MPSNLIVILFDPASPGPPGVDENTDMASRLETCGFLVNHCADPR
                     WLPDFITHAGVGACLILNGAPDQNCLAASSVRMRHPDLVIVVMVPSDGEDALIQALRS
                     GADACCPQGASAALLATLLSTVFRRIRQSAGRWPFRGWSLQERDWVLVGPDDCRVSLT
                     TGERAFLRALLHAPGSRASHGDLAAAVNAAYGAEAPQRVRQIGLLVSRMRRKFEDRGV
                     ALPLRSVHGWGYMFTQGASDQR"
     gene            40314..40709
                     /locus_tag="VFR20_04595"
     CDS             40314..40709
                     /locus_tag="VFR20_04595"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0229827.1"
                     /translation="MSKFADRLRQARLLRGFTQASLAQACGLSQSAIANYETKNRKAA
                     KDVFKLADVLRVNAVWLGQGVGPMETAPTSLPSPSGSRIAEAHVGRQRDHWPFNNIPA
                     DQFWTLPEGDRQLIEDAVASLIASLRRSR"
     gene            40982..42895
                     /gene="thiC"
                     /locus_tag="VFR20_04600"
     CDS             40982..42895
                     /gene="thiC"
                     /locus_tag="VFR20_04600"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEU0229828.1"
                     /translation="MNANPDFLASSATVDAAAVEPLPRSRKIYETGSRPDLRVPFREI
                     SQDDTPTMYGGEANPPLTVYDASGPYTDPEAVIDIRAGLPALRANWIAERGDTEALDG
                     PTSRYGQDRLANKQLDALRFQLKRKPVRARDGRNVTQMHYARQGIITPEMEFVAIREN
                     LRREQYIESLRASGPEGDKLARRLLRQHPGQSFGAATPTAITPEFVRDEIARGRAIIP
                     ANINHPETEPMIIGRNFLVKINANIGNSALGSSISDEVEKMTWAIRWGGDTVMDLSTG
                     KNIHETREWIIRNSPVPIGTVPIYQALEKVDGKAAELTWEIFRDTLIEQAEQGVDYFT
                     IHAGVRLPFVPMTANRMTGIVSRGGSIMASWCLAHHKESFLYERFEDICEIMKAYDVS
                     FSLGDGLRPGSGYDANDEAQFAELKTLGELTQVAWRHDVQVMIEGPGHVPMHLIKENM
                     DMQLEHCHEAPFYTLGPLTTDIAPGYDHITSGIGAALIGWYGTAMLCYVTPKEHLGLP
                     NKKDVKDGIITYKIAAHAADLAKGHPGAAVRDNALSKARFEFRWDDQFNLGLDPDTAR
                     DFHDETLPKQSMKVAHFCSMCGPHFCSMKITQDVRDYARKQGIGESDALAEGMREKAG
                     EFLEKGASIYQRA"
     gene            complement(42947..43603)
                     /locus_tag="VFR20_04605"
     CDS             complement(42947..43603)
                     /locus_tag="VFR20_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU0229829.1"
                     /translation="MRTMRISQAVACLAVAGLLGGCANAPQMSQGGKTAAGAGIGAAL
                     GAGIGALIGNGHGALIGAGIGAVAGGIAGYNWSGIKKDVQQSGASQLGVDVTEMPDGT
                     LKVNIPSNVSFDTNKYDLKPALLPVLNSVARALVQHPELRAKAIGYTDSTGSPAYNQT
                     LSLNRAQAVTNYLSSQGVAGSRLSIEGRGPNDPIASNSTAAGRAMNRRVELYLYAVKS
                     "
     gene            43892..44566
                     /locus_tag="VFR20_04610"
     CDS             43892..44566
                     /locus_tag="VFR20_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0229830.1"
                     /translation="MIEFQHVFKSYGRGRNILADINFRIEPGEFIFVSGPSGAGKSTL
                     LKLIGGLEPPSRGQVHVNGHRIDKMSARARPYLRRAVGVILQDTHLLYDRNAIQNILL
                     PLTVVGLESGAALTRARAAVEKVGLSGKETLNPVEMSGGEQQRLAIARAIVNRPAILI
                     ADEPTASLDQDSAHRIMDVFRDFNRVGVTTLIASHDLRLMASYATRTLHIDQSGRFSD
                     FKGVAA"
     gene            44563..45471
                     /locus_tag="VFR20_04615"
     CDS             44563..45471
                     /locus_tag="VFR20_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0229831.1"
                     /translation="MNRWLRQHRYAAMAAVRRLLAHPFSSLANLLVIALTLAIPIIAA
                     SILASAQPVARQISVSPEITLFMKPGAPASAATQAAARIRKQAPSAIRDVRIVPRQQA
                     LARLKADPAWADALAALPDNPLPDAVIVTLDNGPDLASHAGKLAEQWKQWDGVGHVQL
                     DSDWVRRLEAMLGFLRVGLAVLALGVALVVLATVFNTVRLQALMQREEIGAARLVGAT
                     EAFVRRPFLYLGALTGVVASVVAIGLSAIALHPLNAALARLGQSYGTQLSLHLPSSVN
                     LVLAVVVVAILAALSARWSVTRNTRF"
     gene            45474..46538
                     /gene="mutY"
                     /locus_tag="VFR20_04620"
     CDS             45474..46538
                     /gene="mutY"
                     /locus_tag="VFR20_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU0229832.1"
                     /translation="MLNPVPDTANLASRIVRWQRQSGRHDLPWQGTRDPYRIWLSEIM
                     LQQTQVTTVIGYYQRFLRRFPDVATLAASTEDEVMPYWAGLGYYARARNLHRCARVVV
                     TDWNGKFPPDATEISQLPGIGRSTAAAIAAFAFGARRPILDGNVKRVFTRYFGIEGDP
                     GQRAIETSLWALAEDVVANAPPDLDMPAYTQGLMDLGATLCTRARPGCDNCPLATDCY
                     ARRTARQGDFPNRKTRKARPERHCRMLVLESGGKILLERRPAPGIWGGLWCLPQYDEP
                     GDLTVALSRWGLDKSQARPLEPIKHDFTHFRLHIEPWLAHTANAALAEPVATQNWVAA
                     GDLDATALPAPVKKLLARLF"
     gene            complement(47219..48517)
                     /locus_tag="VFR20_04625"
     CDS             complement(47219..48517)
                     /locus_tag="VFR20_04625"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEU0229833.1"
                     /translation="MQLSDNKATLSFSDGSPSIEFPVYQGTIGPDVVDIRKLYSQAGM
                     FTYDPGFLSTAACESGITYIDGDKGQLLYRGYPIDQLAQKCDFLDICYLILNGDLPGG
                     QQKKDFDSLVTHHTMVNEQIHVFLRGFRRDAHPMAVLTGLVGALSAFYHDSTDITNPH
                     HRDVSAIRLIAKMPTLVAMAYKHSVGQPYIYPKNDLSYTGNFLRMMFATPCEEYHVNE
                     VVERALDRIFILHADHEQNASTSTVRLCGSSGTNPFAAIAAGVACLWGPAHGGANEAC
                     LQMLEHLQANGGIEKVGEFMEQVKDKNSGVRLMGFGHRVYKNYDPRAKLMQQTCKEVL
                     SALGLEKDPLFKLAMELERIALEDEYFVERKLYPNVDFYSGIVQRAIGIPTSLFTAIF
                     ALARTVGWIAQWSEMLSDPNYKIGRPRQLYTGSVVRDVKR"
     gene            complement(48535..48795)
                     /locus_tag="VFR20_04630"
     CDS             complement(48535..48795)
                     /locus_tag="VFR20_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEU0229834.1"
                     /translation="MKPLSDLDRARLRWRARRGLLENDLILTRFLDQYEAHLTDVDVS
                     ALTLLFEMGDNDLLDILLARAEPQGCYDTPDIRRLIGQMRAL"
     gene            complement(48815..49531)
                     /locus_tag="VFR20_04635"
     CDS             complement(48815..49531)
                     /locus_tag="VFR20_04635"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEU0229835.1"
                     /translation="MSEKRIVKFEIYRYDPDKDERPYMQKLDVELHPGDKMLLDAILR
                     IKNDVDDSLTLRRSCREGVCGSDAMNINGKNGLACTTNLLELKEPIVLRPLPGLPVVR
                     DLVVDMTDFYNQYHSIKPFLINDQPPPERERLQTPEAREELNGLYECILCACCSTSCP
                     SFWWNPDKFVGPAGLLQAYRFIADSRDEATNERLDNLDDPYRLFRCHTIMNCADVCPK
                     GLNPSHAIGKIKEMLARRSL"
     gene            complement(49545..51323)
                     /gene="sdhA"
                     /locus_tag="VFR20_04640"
     CDS             complement(49545..51323)
                     /gene="sdhA"
                     /locus_tag="VFR20_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEU0229836.1"
                     /translation="MAAVKKSIPHRRFDVVVVGAGGAGMRCSLQLAQAGLSVAVLSKV
                     FPTRSHTVAAQGGISASLSNMSQDNWYWHMYDTVKGSDWLGDQDAIEFMCREAPNAVY
                     ELEHYGMPFDRNPDGTIYQRPFGGHTANFGEKPVQRACAAADRTGHALLHTLYQRNVA
                     ARTQFFVEWMALDLLRNEAGDVLGVTALEMETGDIYVLEAKTTVLATGGAGRIWAATT
                     NAYINTGDGLGMAARADLPLQDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNKDGER
                     FMERYAPTLKDLAPRDFVSRSMDQEIKEGRGCGANKDHILLKLDHLGADTIKKRLPSI
                     REIAIKFANVDPIKEPIPVVPTIHYQMGGIPANVQGQVLNYANGQNKVVNGLYAIGEC
                     AAVSVHGANRLGTNSLLDLVVFGRAAGNHIVASHPERQHMHQPIPQSSIDHSLERVDA
                     LENRKSDETVSDVGNAIRVSMQHHCGVFRSQDLLNEGVEQIENLAARVANVSFKDKSK
                     VFNTARCEALELTNMIEVALATTKSAASRHESRGAHSLKEYPERDDKEWLRHTLWFSE
                     GRRLDYKPVELKPLSVDSFPPKARTF"
     gene            complement(51327..51710)
                     /gene="sdhD"
                     /locus_tag="VFR20_04645"
     CDS             complement(51327..51710)
                     /gene="sdhD"
                     /locus_tag="VFR20_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEU0229837.1"
                     /translation="MATQERIGTKRLLVGAHYGARDFIVQRVTAVIMAVYTVVLCIGL
                     IFTSNLDFAGWRNLFSFHCIGLPMGQILASLAFLSLAWHAWIGVRDIWMDYVKPTGVR
                     LLLYVLTILWLVGTVVYFLNILWSL"
     gene            complement(51710..52123)
                     /gene="sdhC"
                     /locus_tag="VFR20_04650"
     CDS             complement(51710..52123)
                     /gene="sdhC"
                     /locus_tag="VFR20_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEU0229838.1"
                     /translation="MSDSAPKPRPQYRNISISQILSYRLPLAGKSSIMHRLSGALLFL
                     CLPLVIVPLFALSVTSPESFAAMHAWVAHPVCKIILLILIWAFLHHFCAGIRYLTIDL
                     HLGYDKITAQKTAGVVFGVSLALTVIFGLKLFGAW"
     gene            complement(52224..52994)
                     /locus_tag="VFR20_04655"
     CDS             complement(52224..52994)
                     /locus_tag="VFR20_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0229839.1"
                     /translation="MPDAPLPDRPNAPERGAHSAAFSPLYQQIKGLLLQSLDRGEWKP
                     GEAIPSEFELASRFQVSQGTVRKAIDELAAENLLLRRQGKGTFVATHSEAKVRYRFLR
                     LTPDDGKPPVSGSRILDCRRLKAPADVASLLELRASDTAVNLRRLLSFDQIPTILDDI
                     WLPGSVFRGLTAESLARYRGPLYALFESEFGISMVRADEKIKAVPATVEQADLLEVEP
                     GGPLLLVERLSYTYGDRPVEVRRGLYVTERFHYRNSLN"
     gene            53351..54340
                     /locus_tag="VFR20_04660"
     CDS             53351..54340
                     /locus_tag="VFR20_04660"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEU0229840.1"
                     /translation="MSKPAMRVAVTGAAGQIGYALLFRIASGEMLGKDQPVILQLLEI
                     PDEKAQNALKGVMMELDDCAFPLLVGMTAHSDPRTAFKDADVALLVGARPRSKGMERK
                     DLLQVNAQIFTAQGKALNDVASRNVKVLVVGNPANTNAYIAMKSAPDLPAKNFTAMLR
                     LDHNRALSQLAAKSGKPVGDIEKLVVWGNHSPTMYPDIRFATVGGQSLAKLINDDAWN
                     RDTFIPTVGKRGAAIIEARGLSSAASAANAAIDHVHDWLLGSNGKWVTMGVPSNGSYG
                     IPEGIVYGVPVTTVNGEYTRVEGLQIDTFSRERMDFTLKELLEEREGVKDLLA"
     gene            54504..55397
                     /gene="prpB"
                     /locus_tag="VFR20_04665"
     CDS             54504..55397
                     /gene="prpB"
                     /locus_tag="VFR20_04665"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEU0229841.1"
                     /translation="MTRPTSAGALFRQALAQEQPLQVIGAINANHALLAKRAGYRAIY
                     LSGGGVAAGSLGLPDLGINTLDDVLTDVRRITDVCDVPLMVDIDTGFGPSAFNIARTV
                     KSLIKFGAAACHIEDQVGAKRCGHRPGKEIVSAPEMVDRVKAAVDARTDSDFFIIART
                     DAIAVDGVDAALERAVACAEAGADAIFAEASYDLDTYRRFTKTLNVPVLANITEFGKT
                     PLFTVEELAGAGVGIVLYPLSAFRAMNKAAETVYTAIRRDGHQKNVIDIMQTREELYD
                     RIGYHEYESRLDALFQQGKSK"
     gene            55416..56579
                     /gene="prpC"
                     /locus_tag="VFR20_04670"
     CDS             55416..56579
                     /gene="prpC"
                     /locus_tag="VFR20_04670"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEU0229842.1"
                     /translation="MATTSSTEKAAFKPKKSVALSGVVAGNTALCTVGRSGNDLHYRG
                     YDILDIAETCEFEEVAHLLIHGKLPNKAELVAYKGKLRRLRGLPAQLQVVLEALPAAS
                     HPMDVMRTAASVLGCVLPEKDDHNLPDARDIADRLMASLGSALLYWYHYSHNGRVIDV
                     ETSDDSIGGHFLHLLHGAEPSEEWVRAMHTSLILYAEHEFNASTFASRVIAGTGSDMY
                     SAIAGGIGALRGPKHGGANEVAFEVQKRYETPDDAEADIRRRVENKEVIIGFGHPVYT
                     VSDPRNKIIKDVARKLSKKAQNTKMFDVAERLETVMWDIKKMFPNLDWFSAVSYHMMG
                     VPTAMFTPLFVIARTAGWSAHIIEQRIDNKIIRPTANYVGPEDRKFVALEKRK"
     gene            56602..58053
                     /locus_tag="VFR20_04675"
     CDS             56602..58053
                     /locus_tag="VFR20_04675"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HEU0229843.1"
                     /translation="MSAPISNIRPAPDQVLVDIVDYALDYQIKSELAYETARNCLIDT
                     LGCGLEALEYPACRKLLGPIVPGTIVPNGAKVPGTQFQLDPVQAAFNIGAIIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVAVGKAPLTMRDVLTGMIKAHEIQGCIAL
                     ENSFNKVGLDHVVLVKVATTAVVSQMLGLSRDETINAVSLAWVDGQSLRTYRHAPNTG
                     SRKSWAAGDATSRAVRLALIARTGEMGYPSVLTAKTWGFYDVLFKGQPFKFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVECAMELHEKLKAAGKTTEDIKKITIRTHEACIRIIDK
                     KGPLNNPADRDHCVQYMVAVPMIFGRLTAGDYEDSIAVDPRIDALRDKMICVEDPAFT
                     ADYHDPEKRSIANALTVEFNDGSAFDELVCEYPIGHKRRRKDGIPLLEAKFRTNLARQ
                     FPARQQQRILGVSLDQKTLEAMPVHEYVDLYVI"
     gene            58178..58453
                     /locus_tag="VFR20_04680"
     CDS             58178..58453
                     /locus_tag="VFR20_04680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0229844.1"
                     /translation="MNPSFEHDAAHQRMTCLVDDSLSVLEYTLSGSLMTITHTGVPTS
                     LRGRGVAAGLTGFALDIARCRGWRVRPLCSYADAFIRAHPEYQDLLA"
     gene            58598..61183
                     /gene="acnB"
                     /locus_tag="VFR20_04685"
     CDS             58598..61183
                     /gene="acnB"
                     /locus_tag="VFR20_04685"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEU0229845.1"
                     /translation="MLENYRQHVAERAALGIPPLPLTAQQTADVIELLKNPPAGEEAF
                     LLDLITHRVPAGVDDAAKVKASYLAALALGKETCALISAEQATRLLGTMLGGYNISPL
                     IELLDHPAMGPVAAEGLKHTLLVFDAFHDIKEKADKGNANAKAVVQSWADAEWFTSRP
                     EVPESLTITVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRPGIEPEEPGKTG
                     PMKFLENLKSKGHLVAYVGDVVGTGSSRKSATNSVLWLTGEDIPFVPNKRFGGVCLGG
                     KIAPIFYNTMEDSGALPIELDVSSMNMGDVVELRPYEGRALKNGAVIAEFQLKSDVLL
                     DEARAGGRIPLIIGRGLTARAREALGLPPSTLFRLPKDPASSGKGFTLAQKMVGRACG
                     LPEGQGVRPDTYCEPRMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYP
                     KPVDVKTHHELPAFISTRGGVALRPGDGIIHSWLNRMLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFKGTMQPGVTLRDLVNAIPLYAIKQGLLTV
                     EKQGKKNIFSGRIIEIEGLPDLKIEQAFELTDASAERSAAGCTVRLNKEPIIEYINSN
                     ITLLKWMIANGYEDERTLGRRIKAMQAWLDNPQLLEPDADAEYAAVIEIDLADVHEPI
                     VACPNDPDDVKTLSEVAGTAIDEVFIGSCMTNIGHFRAASRLLEGKRDIPSRLWVAPP
                     TKMDQKQLTEEGHYGILGSAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNANVFLGSAELAAICSRLGRIPSREEYMAEMGVLQRDSDRIYRYMNFDQIEDFREIA
                     DSVGV"
     gene            61344..64046
                     /gene="acnA"
                     /locus_tag="VFR20_04690"
     CDS             61344..64046
                     /gene="acnA"
                     /locus_tag="VFR20_04690"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEU0229846.1"
                     /translation="MPHNTFNTLKSFKIGGKSANFHSLPELGRTLGVDVARLPVSLRI
                     VLESVLRNCDGQKVTETHVRQLANWKPRAKRSEEIPFVVSRVVLQDFTGVPLLADIAA
                     MRSVAARMGKDPKRIEPMVPVDLVVDHSVMIDFFGTQRALGQNMALEFQRNQERYQFL
                     KWGMQAFDTFGVVPPGFGIVHQVNLEHLARGVHHDKANNLYYPDTLVGTDSHTTMING
                     IGVVGWGVGGIEAEAGMLGQPVYILTPDVVGVELTGELRGGVTATDLVLTVTELLRKQ
                     KVVGKFVEFCGPGTSSLSVTDRATIGNMSPEYGATMGFFPVDDRTIEYFKGTGRTKQE
                     IDALKAYFKAQGLYGIPNARDIDYSQVVTLDLSTVTPSLAGPKRPQDRIELGNVKRKF
                     TELFSEHSTNGFSQPPEKLNEVFTTSAGTQVKNGDILIAAITSCTNTSNPSVLLAAGL
                     MAKKAVEAGLKIPPHIKTSLAPGSRVVTHYLEKTGLLPYLQKLGFNVAAYGCTTCIGN
                     AGDLTPDLNEAINQHGLVCAAVLSGNRNFEARIHPNIKANFLGSPPLVLAYALAGTML
                     RDLMTEPVGKGRHGDVWLGDIWPSNAEVRALLNQALDPEAFRANYGEIRTNPGKLWKA
                     IDGVTGDVYNWPTSTYIAEPPFFEGFGMRPAPMPAINGAHALAVFGDSVTTDHISPAG
                     SIKESSPAGRWLQANGVAKKDFNSYGSRRGNHEIMMRGTFANVRIKNLMLPPNADGSR
                     YEGGETLHQPDGERLSIYDAAMRYINEGKSTVVFGGEEYGTGSSRDWAAKGTQLLGVR
                     AVIARSFERIHRSNLIGMGVLPLQFQGNDSVDSLGISGDETYDISGIENGMKPQQEVT
                     LTIHRKNGESIRVRTLLRIDTDTEVDYYQHGGILPYVLRELLAA"
     gene            64188..65393
                     /locus_tag="VFR20_04695"
     CDS             64188..65393
                     /locus_tag="VFR20_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2863 family protein"
                     /protein_id="HEU0229847.1"
                     /translation="MPRTPPSSPSRLSRDAQHLISLTESLALSGCRLEDTYWENLLGQ
                     HLTKLLRGRRNKHIESVLEYLLDNNLNAYEILVEQAETFSESITLSVGDRDYDVLLIS
                     APLVAWTRYQIPGGVLEANAQTALASKLLACVAAPGARLALIPELVGFEQMPRTFQET
                     WVWAHRLGLAALDAKTEPCDIHKTEDSEGMLADARFLVGAIVVPQGAPLFRWQADAPD
                     TPTREQCLQAWTHECAETLGTLFTGCTLEYMLPDAYYVNSREADRRVRPLALRAAVTW
                     LHTAVGLPPSDVRATIVGCGDSVPEEFRVGFSTRQSNDVIYGCVWPVLSKEESLTDSS
                     DTAQADTPDQIAALLKELGVQDIRRLPGIQPAEYCDDCGAPYFPNPLGEMQHPEIPDE
                     VDLNPVHFH"
     gene            65449..66567
                     /gene="earP"
                     /locus_tag="VFR20_04700"
     CDS             65449..66567
                     /gene="earP"
                     /locus_tag="VFR20_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEU0229848.1"
                     /translation="MPASPGIDLFCRVVDNFGDIGVCWRLARQLGTLTGTGHIRLWVD
                     DLGSFKRIQPGVDALADKQVLAGVEIIHWTAPAPDLAPRDVVIEAFACDPPATFIQRM
                     KQGRHAWIDLEYLSAEPWIESCHARPSRQPDGMIKSFFFPGFTPASGGLLREDGLLDQ
                     RDRWLSEPNLRWRLLRDIGLSASHISQLEAGGFQAFLFCYQHAPTGALIQALEQLGRP
                     GVIIVPEGVAPDLPGSGAGMIHVQRIPFVDQDGFDRLLWSSDLNLVRGEDSLVRATWA
                     GKPLIWHIYPQEEQTHMTKLEAWLGLSNYPARSATTMRAWNRGDADLVRENLQALLQP
                     PAWREWCDACGQRARVLAKHPGLAQSLLDYCAKQLQTG"
     gene            66654..67211
                     /gene="efp"
                     /locus_tag="VFR20_04705"
     CDS             66654..67211
                     /gene="efp"
                     /locus_tag="VFR20_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU0229849.1"
                     /translation="MKSAQELRVGNVVNVGSDALVVQKAEYNKSGRNAAVVKLKFKNL
                     LTGSGSESVVKADEKFDIVVLDKKECTYSYFADPMYVFMDAEYNQHEVEADSMGDALN
                     YLEEGMTVEVVFHAGRAISVDLPTIVVREITYTEPATRGDTSGKVLKPAKTNTGMEIS
                     VPLFCEIGDKIEIDTRTGEYRSRVM"
     gene            complement(67288..67767)
                     /locus_tag="VFR20_04710"
     CDS             complement(67288..67767)
                     /locus_tag="VFR20_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEU0229850.1"
                     /translation="MSLKVFDLQCDSGHVFEGWFGSADSYESQRESGLLTCPVCNSHN
                     VVRKLTAARVNTGAGRDSTPADGRQAPAQAQPVAAPAGRQLAQLQAEMLRQIREIVRG
                     AENVGTRFADEARRMHEGESQERAIRGTATEEERQELARDGIAVMPVPDFLDDDRLQ"
     gene            complement(67835..>68345)
                     /locus_tag="VFR20_04715"
     CDS             complement(67835..>68345)
                     /locus_tag="VFR20_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEU0229851.1"
                     /translation="GFVASGQHMDAPSREAAYSLTGKVVHVADGDTFTLLVDGRRERI
                     RLASIDAPETTKDRERPGQPMAQVARQALADMVAGRVLSLTCFEHDQYGRSVCDVPLG
                     NGSTANQVLVEQGLAWANGAGRGKYMRDPSLPGLEAAARKAGKGLWRDPSPVPPWVWR
                     YRCWKQGHC"
BASE COUNT        13306 a        22639 c        20533 g        11867 t
ORIGIN      
        1 cgtgcacttg cgccatttga gatgggtcgc accctttcgt gggggccata tcaaatgggt
       61 gaatgttctc tacgacgcta atctcaagga gcattaccat ggcaacagcc aagaaggccg
      121 tcaagaaagc ggccgcgaaa aaggccacac ccgccaagaa agcagtagcc aaaaaaacgg
      181 tagccaagaa agccgccgtc aagaaagttg tagcgaagaa agcagtagtc aagaaagccc
      241 cggccaagaa agtcgccgcg aagaaagtcg tagcgaaaaa ggccgtcgcc aaaaagccag
      301 ccgccaagaa agccgtcaag aaagttgtag cgaagaaggc cgcggtcaag aaaacggcag
      361 ccaagaaagc cgtcaagaaa gcgccagcca agaaagccgc tgcgaagaaa accgtagcga
      421 agaaggccgt ggcgaagaag gccgccgcca aaaagcccgc tgcgaaaaaa gccgccgcca
      481 aaaagccggc cgccaaaaag gccgctgcca aaaagcctgc ggccaaggcc gcggccaaag
      541 ccagcaaagc caccgcagcg gcgcccgcgg tcaaggctgc gccgaatccg gccgcatcct
      601 ggccgttccc cacgggtggc cgcccttcgt aagggtcgtc actcttctcg cggggcgcat
      661 ttgcgccggg caatcatgcc ggccaggccc cgcgagccgc atagatgctc caccgcccgg
      721 tctccgggcg gttttttttg ataccccggg aggtgtcata taagtatccc tggggatcct
      781 ctgaacaagt tgagcgggaa gcaggcgcta tgagtcgggg tggggtgcaa ggcgcaaacc
      841 acagcgatag ccggtgctat cgcgaggatt tgcaacgccg cagacagccc aagtcacaga
      901 tgcatgcggc gcggtgactt gttcagagga tccctagtca tgcgacaata aggctctttt
      961 aaaaccccgg ttcatcacat cccatgttcg gcgacacttt cggttttctg atcaagaccc
     1021 tgttctccct gttcgggatc gccctgatca ttcgcgcctg gatgtacgcc atccgacttc
     1081 atccgttcaa tccgtattcg caagccgtgc tgcgaatcac aaactggctc gtccagcctc
     1141 tcaggcacgt gtgcccccag agccaccgtt tcgactggcc cagtatcgtc ggatgctggc
     1201 tcaccgcgct tgtattcctg tcgctgacgt ggctgctcgg attcggcatg gcgccgtctt
     1261 tgagcgtgct tccgggcctg gcgctggcgg ccgtgcttac cgtgctgaag tggacattca
     1321 acctgatttt gtgggcgacc ctgatccagg ccgtgctgtc gtgggtcaac ccgatggcgc
     1381 ccgtcatggc cgtgctgcat acgttgacgg ccccgctgct cgacccgatt cgtcgcatgc
     1441 tgcccaatac cggcgggctg gatttctcgc ctctggtgtt gctgatcatc gcgcaaatcg
     1501 ccatgatcat gctcgggcat gccgtgtatg cggcgttcgg tttgtagatt tcacatgggc
     1561 acgacgcgcg tcggcccgtc accgctgatc aatcgcacac gctggcccac tgaaagcggc
     1621 acatcggcgg cctgcgcgat cacgcgggtt tcgccgttgt ccagacgcac ggtgatttcc
     1681 aggccttgag tcttgccggt ctggttttcg atggcattgc ccaccagagc gcctgcgatg
     1741 gcccctccca ccgcaccaag cacactaccc gtgccgcgcc cgatggagct acccgccaca
     1801 ccacccagcg caccgcccgc caggccgcca aaccctgacg tctgcccttg ctggatgacg
     1861 acattgcgca atgcaacgac tgtgccggta cgcacgatct gttcgcgttg cgcctgtccg
     1921 taggtgtaga cggaacttga cgcggagttg ttggcgcacc ccgccagcac ggacaaagcc
     1981 cccgccacca gggttgccgc gaccaggcgg gccaccactg tcttccgggg tttgaaaatc
     2041 gtggaattca tgaatcgatc tcctgatgaa caacgcctcg acatgcaccc ggcacgccgc
     2101 gaatcgtcgc ggtggcgtgt tcgaaagcca gtttatagca tccggacgta gggtcgctac
     2161 agcgccaacc catatgtttt cttcagccgc gccgcgatct gcgcgcgatc gaaggcgtgg
     2221 ttctgcgggc cctgggaggc cactttgatg gcgcccatga gattggccag gcggcaggaa
     2281 tcgggcaacg cccaaccgcg caccaggccg tacagcaacc ccgctcgatg cgcgtcgccg
     2341 cagccagtgg gatccaccac ggcatcggga atcgccggct ctatcgtggc ctcggtgccc
     2401 gacgtgtaca acgtggcacc ttgcgcacca cgtgtgacca ccaccgcttc aagacccgtg
     2461 gcgatgtccg ccatggtccg gcccgtgcgc tgctcgatca cactggcttc gtagtcgttg
     2521 acggtaagaa tgcgcgccag tttcaacatg cggttcagat cctcgccgga gaacagcggc
     2581 atggcctgtc cgaggtcgaa aatgaacgga ataccctgtg catgcaaccg ctgcgcatgc
     2641 gcgaacatgc cgtccttggc atcgggcgcc acgatagccc aggcggccgc gcgcccggtc
     2701 aggtcattgt cggccgagca tcccatggcg cccggatgga acgcggcgat ctggttgttg
     2761 tccagatcgg tagtgatgaa gcattgcggc gtgaacatgt cgggcagaat gcgtatcagg
     2821 ctggtgtcga tgtcgagctt ctcgaagcgg gccagatagt ccgccgcgtc gcccccgacg
     2881 gtgccgaccg gcaccgggct gccccccaac aatttcaagc tatacgagat attgcccgcg
     2941 cagccaccga actccttgcg catgccagga accaggaatg acacgctgag cgacttgatg
     3001 ttgtcggcca gtatatgatc cttgaaatag ccctcgaaca ccgcgatcgt gtcgaacgcg
     3061 atcgaaccgc aaaccagtac gtcttcagac atgcctaccc tttgctcatg aaaaatattt
     3121 ggccagttga aatccgctga ccttgacccc gttcattgta atggggacca tggcggtgac
     3181 ttcactgcgc gcggcgaacg ggcgttgcgc gacgccgggc ggcaggtaca aggatggcgg
     3241 caggtctttc ttcacaacca ccgtgcccga gaaatccagc agatccagaa ccagggtcgg
     3301 ccattcctgg ggcttgtcga ggctgttgcg caacgtcacc cggaggacca tcgcctcggg
     3361 tggctgcatc gcgggcgatg ccccctgccc tcccagcggg ttggcgcgcg tctgcagcga
     3421 ggaattcatg atcgtgatct ggctgatatc ccgcgaatac ggaacgcggc aatgcaatgg
     3481 cccacacgcc cgctcgagca acggccgcac ggccggtaga tgggcggcga gttgcgcccg
     3541 atagacatac aggcactgcg ccaggaacaa cgccacgccc agcacgctga gaatccccca
     3601 gaagtaaaac gcgggcccgc gataacgcgc gggcagcgga tccaggaaat ccggctcctc
     3661 ctcgggttcg ggtgcggcgg ttcggggctc cacgtagacg ccatatgact ggcgctgggc
     3721 gaggaatggc tctttatcgt ggggcgttgt gtcatcggcg agagcttcat ccggatcggc
     3781 ctcatccgcg tcctccgggg aacgattgga cacatacgga gctcgtgttt ctccccgtgc
     3841 cggacgtggc ccaccgttca cgggcaacga tatcgttggc tctgtggggg cgccggcttg
     3901 cgggcgccgc ccagtgatcg tgaacgctgg ctcggcccgg gaagcctctg tccgcggcac
     3961 ggcatgctga ccgtgatgtt cgacacgctg gcgcaccaca tgcggcaacg tgtcacggcc
     4021 gccctcgccg gggtgaggcg cttgacgggc caccggctca ggtgcctcgg tcacgcgcgg
     4081 aacacgaccc gcatgggaac ccagcgccgg acccggtaca ggcgccacag ggcgagacgc
     4141 cggcgtcgaa cgcccctggc gcccgtcagc gggcaccact gcgtcgaagc cgtcgaagat
     4201 gtgggcgcaa ttggggcagc ggatatagcc cttgcgcagt tgcaactgct cgagactcgc
     4261 ggaaaacgtc gcaccacact gggggcatcg cgtagtcaaa tccataagcg agatctatcg
     4321 tgatcgttca gttcgttgcc catgcaggca tacccagccc gcccgcgcgc gccagacgct
     4381 gagtgtgagc caaggcgcgt aagcctgagc cacttcgtct gcctgacgct ccagcacacc
     4441 cgatagagcc agccggccac cgggccgcac cctgttggcc agcatgggcg ccagcacctt
     4501 caaggggttg gacaatatgt tggccaccac cacgctggcc tgtgccgagt cagtgtgatc
     4561 aggcaaccgg gcgtcgagat cgaccctgtt gacctgggca ttgtagcggg tggacaacac
     4621 cgcttgttcg tcgatgtcga cggcatgcgt gtcgcccgct ccgagcattt tagccgcaat
     4681 ggccaggata ccggaaccgc agccgtaatc gagcactgtg tcgccgatgc gcacgtgctc
     4741 ggccaaccac tccaggcaca aatgcgtggt ggggtggctg cccgtgccga acgcgagccc
     4801 tggatcaagc tgaatgcgga tagggttgtc agcgtcagag gcggggtggg cgacgccggt
     4861 gggcactgcg ggatcgttct ggtgccagct gggcacgatc cagatacgcc cccccacatg
     4921 aatggggccg aactgggact gggtcagacg cacccagtcg gcgtcgggca cttcgcggat
     4981 cgaccatcca gcttgaggag ccaggccgga caaggccgcc gcctgctcga cgatctgcgc
     5041 cggatcggcg ccgtcgggca aaagcgcggc aagccgatta cgccgccaag cctgaacctc
     5101 gggttcgaac cccggctcac cgaacaacgg ttgctcgtgg gccgtgtcga catcggcgtc
     5161 ttcgaccgac accgacagca ccccggcctc gatcagcgca tcggacaatg cctcggcctg
     5221 cgcttccaga caatgcaata ccagctctcg catggtcgtt tccgtataaa aaaccccacg
     5281 ccacgcaggc caacgtcagc ttgctggccg cgatcgatca ggatcgctgg gccagtttgt
     5341 gctcaagata atgaatgctc gtcccgcctt tgatgaagtt ggcgtcgtgc atgagatcgc
     5401 gatgcagcgg gatgttcgtc atgaccccgt cgaccgccat ctcggagagt gcgatatcca
     5461 tgcgggcgat cgcctgctcg cgcgtgggcg cataggtgat cagcttggcg atcatggaat
     5521 cgtagttggg cggcaccgtg tagccgctga acacatgcga atccatgcgc acccccggcc
     5581 cgccaggcac atgccagttc gtgaccttgc ccgggctggg cgtgaacttg tagggatcct
     5641 cggcgttgat gcggcattcg atcgcgtgcc cgcggagctc gacgtcgcgc tgacgcagtg
     5701 tgaacttttc accggcggcg atgcgtatct gcatctgcac cagatcgagc ccggtgacca
     5761 tctcggtgac cgtgtgttcg acctggatgc gggtgttcat ctcgatgaag tagaactcgc
     5821 cgttttcaaa caggaactcg aacgtgccgg cgccgcgata accgatcttg cggcaggctt
     5881 cggcacagcg ttcgccgatg cgctcgatca gcttgcgttc gatgcccggc gccggagatt
     5941 cctcaatgac tttttggtgg cgacgctgca tggagcaatc gcgctcaccc agccacacgg
     6001 cgttgcgctc tccgtcggcc atgacctgga tctcgatgtg gcgcgggttc tcgaggaact
     6061 tctccaggta gacctcagga ttgttgaagg cgatgccggc ctctgatttg gtgagcgtga
     6121 cggcgttgtg cagggcggcc tcggtgtaga ccacgcgcat gccgcgcccg ccaccaccgc
     6181 ccgccgcctt gatgatcacc gggtagccga cgtcacgggc gatgcgctgc acttcggccg
     6241 gatcgtcggg cagcgcgccg gccgagccag gcaccaccgg cacgccggct tcgatcatcg
     6301 cctgcttggc actgaccttg tcgcccatga ggcggatgga ttcgggcttg gggccaatga
     6361 agacaaatcc gcttttctcg acgcgttcgg cgaagtcggc gttctcggag agaaaaccgt
     6421 atccggggtg aatggcttcg gcgtcggtga cctcggcggc cgagatgatg gccggcatgt
     6481 tgagatagct gtcgcgcgcc ggggcgggcc cgatgcaaac ggattcgtcg gccaatctca
     6541 cgtacttggc ctcgcggtcg acttccgaat gaacgaccac ggtcttgatg cccatttcgc
     6601 gacaggcgcg ctgcacccgc agggcgatct cgccacgatt ggcgatgagg attttctcga
     6661 acatgggctc agccaatcac gaacaggggt tgaccgaact cgacgggttc accgttttcg
     6721 accaggattt ccttgaccac gccggccttg tcggtttcga tctcgttgag cagcttcatg
     6781 gcttcgatga tgcacagggg ctcaccctct ttcacgctct ggcccagctc aataaaaggc
     6841 gaagcgcccg gattgggcga gcgatagaac gtgccgacca tgggcgattt gatcacgtgc
     6901 ccctggggca cggcgggcgc ggccggagcg gccggagcgc cggccacggg gggcgccgcc
     6961 atcaccggcg cggccatggg cggtggcgcg taagccacgg cgggagtcgc cggggagaat
     7021 ttgacgatct tgaccttgcc ttccccttcg gtgatttcga gttcggcgat tcctgattca
     7081 gcgaccaggt cgatcaaggt tttcagtttt ctgagatcca tacagatttc ccgtgacatt
     7141 gccccgcgcc atacggacgg ggccgacgtt gattgaatac aaatattgaa actagctcga
     7201 ctaaccaatg ccgctggagg cctcaggcct ccagcgcgta acgcatggcg gcttcatacc
     7261 cgaacgcgcc cagacccacg atcaccccct gggccagatc ggacaggtat gaagaatggc
     7321 ggaatgcttc acgccgatgg acgttggaca ggtggacttc aatgaaccgg atcgccacgg
     7381 cggccagcgc gtcgcgcacg gcgacactgg tgtgagtata ggccgccgca ttgatgatga
     7441 tgaaatccgt ctggtcgccc gccgccgcct ggatacggtc aaccaggccg ccttcgtgat
     7501 tgctctggaa acactcgcaa ccggcgccct cgcgagccgc cagaccctgc agattccgat
     7561 cgatttccgc cagagtctga tgtccataga cactgggctc gcgtgtgccg agcagattca
     7621 ggttggggcc atgcaagacc aatatgcgtt gcgccattat ggaacactaa aggagacaga
     7681 ccgtcttttt acgctaaaaa ggcctatttg tccattaaaa cacagactat ttgccatacg
     7741 aaagcatcca gaaccttaaa acggcccttc cagaccacgc ggtttcaggc taccaggcca
     7801 tgcaccttcg caccgaatga atcggtcttg atctgaccca cgacccgatc acggatcatg
     7861 ccctggcgat cgaaagtcac tgtgaacggt attccgcctg tggtatcgcc caacgcgcgg
     7921 acgagttcga taccgccatg gccgacgatc agcagcggat aggaaaccgg aactttttgg
     7981 atgaattcgc ggacgttgtt cgcggtatcg accgcgagcc ccagaaaccg cacgccggga
     8041 tactgttttt gcaacgcatc gagttcaggc atttctttca cgcagggcgg acaccacgtt
     8101 gcccagaaat tcacgatcag gggtttgccc agatagctgc tcatggcatg cggagtgccg
     8161 tgcacatcgc ggaacgattg accgaacaag cccgtgtcgg ccaaggcgcg ccggggccgc
     8221 cacgcgccaa gaaaggccgt ggccgccacc ccctgaaata tgaaatcgcg ccgcttcatg
     8281 cgttttccga aagttcgtca tgcttttttc atcatagcac cggcctgccc gatacccggc
     8341 ggggagccac tacaataacg ggtggaggac gtcatgcatc tacatatcct gggaatctgc
     8401 ggcacattca tgggcggact ggccctgatc gcgcgctcgg ccggccatac ggtcaccggt
     8461 tgcgacgccg gcgtctaccc gcccatgagc acccagctgc aggaacaagg cattcacctg
     8521 atcgaggggt tcgacgccga tcaactgcgc ctccggccgg atctgttcgt cgtgggcaac
     8581 gtggtctcac gtggcaaccc attgatggaa gccatcctgg atcagggtct gccgtacacc
     8641 tcgggcccgc aatggctggg cgaacacatt ctgcgcggcc aacacgtgct ggccgtggcg
     8701 ggcacacacg gcaagaccac aaccagcgcc atgctggcct ggatactcga acacgccggc
     8761 ctggccccca actttctcat cggcggcatc gccggcgacc tgggagtgtc ggcgcgttac
     8821 gacgcccagc agacggtgtt cgtcatcgag gccgacgaat acgacaccgc gtttttcgat
     8881 aaacggtcga aattcgtgca ctaccggccg cgcaccgcca ttctcaacaa cctggaatat
     8941 gatcacgccg acatctttcc cgatctagcc gcgatcgaaa cccagtttca tcatctggtg
     9001 cgaacaattc cggcatcggg ttgcgtcata cgccccgtcg actcacccgc catcgatcgg
     9061 gtcttggcgc acggctgctg gacaccggtc gacaccttcg gcccgggtgg ccactggcag
     9121 gccgccggcg acatcgacaa cgagttcgaa gtgcggcacg gcgccgagca cgccagcatc
     9181 gcgtggggat tgagcggcac ccacaaccgc atgaatgccc tggccgccct ggccgccgcg
     9241 tcacacgcgg gcgtatcgct gcggacgggc gtggaggcgc tgtgtcggtt ccagggcgtg
     9301 aggcgccgca tggaattgcg cggcacggtc aaccagatca gtgtctacga cgatttcgcg
     9361 catcacccca ccgccattca gaccacactg gaaggcgcgc gcaacaaact ggccggcggc
     9421 cgcattatcg cggtgctgga accccgctcc aacaccatga agctgggcac catggcgtcg
     9481 cgccttccga aagcgctcga ggcggccgac cgcgtgttct gtttcggcgc gctctcgggc
     9541 aaacacgcgc tcggctggga cccagcgcaa atcctcgccc ccctgggcgc gcgtgccagc
     9601 gcctacgacg acttggatcg catggtgcgg gccatcaccg ccgaaagcca gcccggcgac
     9661 gccatcgtgg tcatgagcaa tggcggcttc ggtggcattc atcagaaact cctggatgcg
     9721 ctgcgaatcc gtgggagtgc ggcatgatcc tgtacctgca cggcttccgt tcctctccgc
     9781 aatcgttcaa ggccacgctc ctcgcccagg ccatgcgtgc gcgtggcctc gaagacgcct
     9841 ggttgtgccc gcaattgccg gcgagcccag cgcaagccat ggccctggcc gatcgcctga
     9901 tacgcaaggc gctgccgggc ggtatcgagc agacacgagc cgaactggtc atcatcggct
     9961 catccctggg cggctattac gccacgtgcc tggccgaaca ttggcaatgc cgcgcggcgc
    10021 tgctcaaccc ggttgtgtac gccgcgcgcg atctggccac ccaggtgggt gaacatacgc
    10081 tgtatcacgg tggcggccca ttcacgttcc tgccggaata cattgacgaa ctggcggaaa
    10141 tggcggtggg gcgtcccacg caccccgaac ggtattacct gctggcgtcc accggcgacg
    10201 aagtactcga ttggcgggaa atggcggact ggtttcaggg gggccgtcag cacgttataa
    10261 acggcggcga ccacggcctc tcggactttc cggcccacct gccagaagtc ctggaattcg
    10321 ccctgaccgg cacctgaccg ttcattcgat tcccatagaa tttccccgcg acaccacgcc
    10381 cactttccaa tcccccatgc acgtactcta cgaagacaac ggcggtttca aggccggccg
    10441 catattttcc gagggcgaat cgaccatgca ggtcgaagcc gaatcgggca agcgcagcaa
    10501 gatcaagaac gcaagcgtgc tgttccgctt cgaccagccc gcgccggcag ccctgctcga
    10561 acaggcccag gccctggccg acacgctgga aaccgatttt ctttgggaat gcgcccctca
    10621 ggaagaattc ctggccgccg acttcgccga ggactatttc ggacatcctc ccagcgccgt
    10681 ggaaaaagcg gcgctgatct tcgccctgca caatgccccg gcctatttcc accggcgtgg
    10741 caagggcggc tatcgacccg ccccgcccga catcctggcc gccgcgctgg ccgccatcga
    10801 caagaagcgc aagcaggccg agcaacagca ggaatggaca gacgcgctcg tcgcccatca
    10861 catgcccgcc cccatcgccg aggccgccac cgatcttctg atccgcccag acaagaattc
    10921 catgcaatgg aaagcgttgg acgctgccgt caagcaactc gccaccactc ctgaaaagct
    10981 gttgctggaa gcgggggcgt ggccccaccc gctggcgttg catcgtgcgc ggtttctggc
    11041 cgccaatttc ccgcacggca cgacgctgcc gccggtggag gtcggtacgc ccggcggcga
    11101 cctccccttg tcggacgtca tcgcctactc ggtcgatgac atcagcacca ccgaggtcga
    11161 cgatgccatc tcggtcacca tgcaagggga tgaccgcgcg ctcatcggca tacatatcgc
    11221 cgttccggcg ctggccgtga cacgcgacaa cccgctggac aggctcgccc gtgaacgcat
    11281 gtccacggtc tacgccccgg gtcagaagat ccccatgctg ccggacgcat tgatcgaagc
    11341 cttttcgctg gatgaaggca aaccccggcc ggccctatcg ctgtatgtac aggccgatct
    11401 ggggagtgga gaaatacttt cgagcgaaac ccgcctcgaa cgcatacagg ttcgtgagaa
    11461 cctgcgccac aaccacctcg acgagctgat cacggaagag gccctggacg atcctggcgc
    11521 ggatctcccc tacggtgaat ggctgcgccc cctgtggcgt ttcgcgcggc atctcgccac
    11581 ccagcgggaa caagtgcgcg gcaaacccga gaacaacaac cgcatcgact accagttcta
    11641 cctggaaggc ccggccgacg acccggacgc gcgtgtcatt ctggccccgc gccgccgcaa
    11701 tgcccccctg gaccgcctgg tcgcggaata catgatcctc accaacaacc tgtggggcgg
    11761 cctgctggcc aggcacgggg tgcccggcat ctaccgctcg caacaggctg ggcgcgtgcg
    11821 catgtccaca caggctctgc cgcacgaggc catcggtgtg ccgcagtacg cctggaccac
    11881 gtcgccgctg cgccgctacg tcgacctgat caatcagggg cagttgctgg caacggtcga
    11941 gcatggcgtg tcggcaagac tggtggcccc cttcaaaccc aaggatgccg acctgttcgc
    12001 catcatcggg gcgttcgatt cgcagtacgc catctggggt gactaccaga ccgccatgga
    12061 acgttactgg tgcatgcgct ggctacagca gcaaggtctg cgccgcgtgc gcgcgaccgt
    12121 catccgcgac gatctggcac ggctcgattg cgcgccactg gtgacgcgcg tcagcgccct
    12181 gcccgcgctg gaacgggggc aggccatcac ccttgacatt tcaggttacg atgaattgaa
    12241 cttggaaatg gattgccgtt atgtcgaaac ggtgtccgat taaagcccag gatctgttca
    12301 caccaacctg cgattcccgc attgaacccg ccggaccccc cgccctctcc cgcggcgccc
    12361 ctcaaccctg cccgcccccg gcggcccaga ggttacatgc gtgtcgcact gccgatttcc
    12421 ctcgcgctgc acgcggcgct gctggccgtg cgtttttcgt ctccgccacc gcccgcgcca
    12481 gaggccagca cgctggaggt ggtgctggtc aatgcgcgca ccaaggccgc tccgatcaaa
    12541 cccagactgc tggcccagaa caccctcaac ggaggcgggc cggccacttc gggctacagc
    12601 gcctcgccac tgccgcgcac caccgaagac cagaccgcga accaagtcgt gcttgccgcg
    12661 ctccgacaac gccaggcacg gctggagaag gaacaaaagc agttgttcac gctgttggtg
    12721 gacaggcaaa agacactact ggcccagatt gaacgcgatg tcccggatga ttccaccgac
    12781 gcgggcgaag acgaacgcac ccaggaagcg ctggtggtga acgcgcagat cagcgccttg
    12841 aaggcagcca tccagcgcta tgacgcgcgc cctcgccagc gcttcgtggc accatcgact
    12901 caggcggttc cctatgccaa atacgtggag gcgtggcgca agaagatcga gctgctgggt
    12961 acggaacact atccggaaca agcccgcggc aaggtctatg gcaaactgcg catgaccgtg
    13021 tacatcaaga agaacggcga actgctgcgt gtggagatcg accagccatc cagccacccc
    13081 atcctgaact cagccgccaa gcgcatcgtg caactggccg cgcccttcgc cccgttccca
    13141 cccgccctcg cgaagaacac cgatgtgctg gccatcaccc gcacctggca tttcgtgaac
    13201 agcgaactca ccaccagccc acaatgaccg aacccacccc gccccgccgc tgcgccgtca
    13261 taggcaaccc catcgcccac agccgatcac cgttcatcca cctgcgattc gccgaacaaa
    13321 cggggctgga tctgcgctat gacaggatcc tcgccgaggc cgatgatttc gaggcggtgg
    13381 ccacggattt cttccatggc ggcggcatcg gcctgaacgt gaccgtgccg ttcaaggaaa
    13441 aggcgttcac gctgacccac cgtcggcttg atgacagtgc gagagtggca caggctgtca
    13501 acaccctgta tgtgcgcgag ggcgtgttgc atggcgccaa taccgacgga accggcctgc
    13561 tggccgatct ggaacgcctg ggttgtacgc tatcgggcaa gcgcgtgctt ctggtgggag
    13621 ccggcggcgc cgcccggggt gtcgcggccc ctctgctggc gtcgggatgc gcgctcctgc
    13681 atgtcgtcaa ccgccacgcg gagcgggcgc acattctgcg ccagcatatc catgaaatat
    13741 ctccggccac tgcggggcgg ctctcgagtg gcggcctgcg ggacgccgac ggggactggg
    13801 acctcatcat caacgcgacc tccgggagcc tgcaagggca ggctcccgac attccggccg
    13861 cgcgctatgc ggcgggcgcg ctggcgtacg acctggtcta cagcgcccgt cccacaccat
    13921 tcatgatcca ggcgtcgcgt cttggcgcgg atcaggtgtc cgacgggctc ggcatgctgg
    13981 tgtcacaggc ggcggcgagc ttcgccatat ggcatggccg ctatcccgac gcccttcctg
    14041 tgctcgccgc gctgcggcaa gcgctcgacg ccgagacccg atgaacgcgc gacgcaagcg
    14101 tcgatcatcc cgtcacggcc tcgtccgcgc agtcgtgctg ataggcatat cggccgttct
    14161 gctgtacctg ctcggcctgt tcctcatggt tgtctggtac aacttccgca acccggatac
    14221 cagtgccttc atgcgcgaga cgctgatcga actgcgcgcc acgaatcccc atgccacgct
    14281 ggttcatgaa tgggtgccct atgcggggat cagcatcaac ctgaaacggg cggtgatcgc
    14341 ttccgaggat tccaccttcg tcgagaacga tggcgtcaac tgggacgcca tacgcgaggc
    14401 atggctctat aaccagcgcc agaagcgacg tggcagcgac aagcggcgcg gcggctcgac
    14461 catcacgcag caactggcga agaacctgtt cctctccagc tcgcggacct atctgcgcaa
    14521 ggggcaggaa ctcattctgg cctatatgat caacgccatc atgagcaagc ggcgcatcct
    14581 cgagctgtac ctgaatctgg cgcaatgggg cgacaacctg ttcggcgccc aagccgcggc
    14641 gcggcgctat taccatatcg atgcgtcgcg gctcagccag cgccaggccg cccaactggc
    14701 cgtcatgctg cccgaccccg attacttcgg cgatcaccgc agttcatcct atctacagag
    14761 acgcacgaac gtcatcgagc aacgcatgcg tctggtggac attcccggtg tgcactaaaa
    14821 tcggctggat gccacgcgac gcccggacgt accgcgtatg cgcaccgcgt tctcccgcca
    14881 caccagccca ccctgaagcc caccccgaca atgcgcaccg cccgccgcta ccttgccctg
    14941 gaaatctacc gcacctgcgc cgtggtgcta ctggcattgg tgggcctgtt cacgttcttc
    15001 gcgctcatcg acgacctcaa caacgtcagc gccacgttct cgctgctcaa tctgttctac
    15061 ctgcaggcat tggccattcc aacccgcctt tacgatctgc tgccaatcgg cctgctgatc
    15121 ggtgccattc tggcgttggc cgggctggcg caacgcaacg aactggttat tctgcgagtg
    15181 tccggggtca gcggcctgaa gctgttgacc atgctctggg cgatcacgat tccgctggtc
    15241 cttggcgctc ttttcttgtc tgaaatcgtc acgccggccg cggagatcaa atccagcgaa
    15301 gccagcctga tgatgctggg ccgctccaat ggcggccgcc ttgacagcgg ttactggttc
    15361 aaggagtcca gtcctgaaac cggcggtacc cgcatcatca acatcaagaa gctcaaggcc
    15421 aacggcggcg tggccggtgt cgtggtctac gaattcaatg agcggcacca actggtgcga
    15481 tactcacagg ccaccgacgg gcgctttgga gatggcaaac tcatactcga ccatgtcacc
    15541 gagaacaaaa tagacggcaa cgccatctcc gcgctcgcca acgcgaagca acccacgaca
    15601 cccgtcacca cggtggatac catcgccgag cgaacattgg ccacgacgct cactcctgat
    15661 cgcctcatcg cccgcatcct cacgcccgag cgcatgtcca tcatcgatct gctcgactac
    15721 atcaactacc tcaaggccaa ccatctgcag accgatcgcc aggtggtcgc gctctggcgc
    15781 aaggtggcct atcccttcac gctactggtg atgatgacca tcgcggcccc catcggcttc
    15841 atgcaaacgc gtcgaggcgg tgtgggcagc aaggtgttca tcggcatcat cctgggcgtt
    15901 ttgttcttca tgctcaacca actcgccctg aacgtgggta tgctcagcaa atggccgcca
    15961 tggatcacgg ccattgtccc caacgtggtg gtgctcctgc tggcgcttgg cgcactgctg
    16021 ctcatggaga accagcatca cgtgcgcgcg ttccggcagc ggaaccaggc gcaaccggcc
    16081 gcatgaacat ggcgcgttcc gaatcgagcg tggcgccgcg ccactccgat cactcaccgg
    16141 atgcgggcac gcaccccccc acgccgatct ggatcatcgg cgaccttcag ggctgctgcg
    16201 ccgcgctgag cgaactgtta gagcatccag acattgccac ggacccgcgg gctcggttct
    16261 ggtttgccgg tgacctggtc aaccgggggc cggactcact cggcaccttg cgttcggtga
    16321 tggcgctggg tgagcgcgcc acggccgtcc tgggcaacca cgacctgcat ctcttggccg
    16381 cggcggcggg catccgatca cccggccgct cggatacgct ggacgacatc ctcaacgccc
    16441 ccgacgccgg cgacctgatc gactggttgc gccaccggcc cctggcgcac tacgagcacc
    16501 accatctgct ggtccacgcc ggtgtgctgg cgggatggag cgtcaccaag acactagagc
    16561 tggcgcgcga agtcgaaatg tccctgcgca gcgccgactg gcgcaagcac ttggcgcata
    16621 tgtatggcaa taaaccggtg cgctggaagg attcgttcag tggccacaag cgacagcgcg
    16681 cgatcatcaa cgccctgacc cgcatgcgca tgtgccaccc caacggcgac atggaattca
    16741 agcacaagcg aggacccgag cccgggcagg aactcatgcc atggtacgac gtgcccgggc
    16801 gcgtggccca cgacgtcacg gtggtgttcg gtcactggtc ggcgctgggc ctgctggtgc
    16861 gccccgatgt catctgcctg gacaccggct gcgtctgggg tggcaaactg accgccatgc
    16921 gcatgcacga ccaccatctg gtgcaggtgg cctgcgcgca attccgcaag ccggggtaac
    16981 ggatcaaaaa gtgtctgcaa tcacggcgcg acgcacgacc ttatgcgccc tggcggccac
    17041 gcccgcgcgg ccaaggccgc gacaatcaga ctccgcgatg accgggagaa attcggcgtc
    17101 gacccgtgcg cccgtggtgc tcaacaacag ccagacgttg tgcgccagcg tctggtctcc
    17161 cacgaacgag aggtggtcgc tgcgccgccc tttgtgtatg aagcgcagcg cgacgggctg
    17221 cacgtcggcc ccagcctgga tcgcgggttc gaacaggctg gcgtgaaacg gcgccacatc
    17281 gaaaccagat gatgacgtcc cctcaggaaa cagacccacc gcatcgccct ggccaaaacg
    17341 ccgccccatc tgctcggcca ccgcgcgaat cgcattgcga cgcccccggt cgaggaacac
    17401 cgtgccggcg ccagccacca gccagccaat gacaggccag cggcgcactt cgcttttcga
    17461 aacgaacgac acgcaacgca cactgttcaa aacgaaaatg tccacccatg acacgtggtt
    17521 cgccacccag agaacgggcc ggtcgagcac gggctggccg gcgatgcgaa cggcgacgcc
    17581 acacagggcc atcagggccg ccgaccacca gcgcgtgacg gcgcggcgca cgcggggcac
    17641 caagggaaag aggatggaca cgcacagcag cccaaataga atccacacca ggaccacgca
    17701 caggcgcgcc acgaaacgca acacgttcac gcgtgccacc ccgtctggag aactttcttg
    17761 accaactgcc gcgcatgcaa gcggctgaac ggtgcggccc atgtcaacac gacgatgcct
    17821 ccagcgcgtc gaaatagcgc tgcaggatga tcgccgccgc catggcgtca tcttcgggcc
    17881 ccgaggaacc gagcatccgt tgcgcctcca tgctggtgtt gcgttcatcg accaaatcga
    17941 catcgagtcc gaaacggcca tgcagttgat tggcgaagcg ccggcaacga aggctggcat
    18001 attgttcgcc gccatcgagg gtcagcggca ggccgaccac cgcgcgatct ggctcccact
    18061 cccgcacgag ccgcgcaatg gcctggaacc gttgctcccg tgtcgtggcg accagggttt
    18121 ccaaagggcg cgcctgccgc gtcagggtgt tgccgatggc aaccccgatt ttcctcagcc
    18181 cgtaatcgaa cgcaagcagg gtctcttcag tcatgccccg cgtcgcccgt cagcatggtg
    18241 gcatcaatgc ccaacaggcc gagcgccgca tcgtagcgtt cggcgggtgg cgtctcgaac
    18301 aggatctcca tcgacgcacg cgtattcagc caggcgttct gcgagatttc gctttcaagt
    18361 tggccggcgc cccaacccgc ataccccagc gtgaccagca cgtggccagg accgtgcccc
    18421 tgcgcgattt cctgtagcac atcgcgggac gtcgtcaggg cgacatcgcc cagttcgata
    18481 ctggaactgt agttgccctt gggcgtgtgc agcacgaacc cgcgatccgt ctggaccggg
    18541 ccgccgaaaa acaccgggtc gtcgcgaatc ggcccgattt cgagcgagag gtcgagttcg
    18601 atgcgctcga gcagattacc caaggtgaga tcggtgggcc ggttgatcac caggcccagc
    18661 gccccttgtt ccgtgtgctc gcacatatag atgacggagt tggcaaggct gccgcccact
    18721 ttgcccggca tcgccaccag aaactgccgg gacaggtcga cgctgcttcc gttaccggag
    18781 cgtggggtgg atggttccat ggctcgccct cctatcgata ggccggtaca cctatcatac
    18841 gcaattacac ctcgatcagc tcgaaatctt ctttgcgcgc gccgcactca gggcacaccc
    18901 aattgggcgg cacatcttcc cagcgggttc ccggcgcgat cccctcttcg ggcaagccag
    18961 cggcctcgtc gtaaatccag ccacaaatca aacacatcca cgtacgcata ctattgttac
    19021 tcataaaaaa ttcgaaaccc gatcatcacg gacattgagc ggttccatta gaatggggcc
    19081 aatcatgccc atttcatccc ctccgctagc actcattttc ggcccattcg atcccagcgg
    19141 gtcaagtaat ttacccgccg acgcggttac ttgcgcgttg ctcgggggcc acgccctggg
    19201 tgtgctgacc ggcatccacg tacaggacac cgccggcatc gaggccatac agcccgtcgc
    19261 ccccgacctc atcgacgacc aggcgcgctg cctgctcgag gacatggcgg tgggcgccat
    19321 gaaagccggc ccgctgtaca cagtcgaatc ggtcagcgcc gtggcgcaga tcgtggccga
    19381 ctacaaccct gttcctctgg tgctgcacct gggggcgctc cccgatgaag aaacgctggc
    19441 cgacggcctg gacgcggagg aactggtcgg ggcggtatgc gaacttctgc ttccccaggc
    19501 cgacctcgtc atagcggatc ataacctgat taccctctgg gccgaacacg gtttcctgaa
    19561 ttcgcgcgac tcgggctccg ccgcggagat cctgctcgaa taccaggcga aatgggcgtt
    19621 gatcaccggc gccccattgc gccccggcca tcggtgctat caactggtgg gggcggacaa
    19681 ccagacccat cactggccgt gggcgccgcc gcccgaacgg gtcaccggcg ccgatggccc
    19741 gctgggctgc gccgtcgcgc tcgaactggc ccgcggccag gaagtccccc tggccgtcga
    19801 aaccgccatc cgccagacgg cggccatggc cgaacagtct ttccagcccg gaatggggca
    19861 tcgcctcatc aacaggtcac atccatgaca cccgccctga agttcccacg cggcctctac
    19921 ggcatcaccc cggaatggga cgacgccaag cgcctgatgc gcgccatcga agcggccgcc
    19981 acgggcggta tgacggccct gcagtggcgc cgcaagacca tcgatcaggc cgacgccctg
    20041 ccccaggcac gcgccgtggc ggcgttgtgc cgtgaactgg gtgtgtgttt catcgtcaac
    20101 gatgactggc gtttcgccgc cgccatcgat gccgatggcg tgcacatcgg acgcgaggac
    20161 ggtcctctgt cggacgcccg gatcgctctg gggccggaca agatactggg ctgctcctgc
    20221 tataacgaac ccgcgcttgc cgagcaggca ctgcgggccg gtgcggacta tgtcgcgttc
    20281 ggcgcgatgt atccctccat ggtcaaaccg ggagcggtgc gcgccaccct ggaccatatc
    20341 cacgccggcc gcgaactggc cacacaactg gccgacgccg gcgccagggc agccgtcatc
    20401 gccatcggcg gcatcacacc cgccaacgcg gcgcccatcg tggccgccgg cgccgacagc
    20461 atcgccgtga tcagcggcct cttcgaggcg cccgatatcc gggccgctgc gcaagcctgc
    20521 agcgcgttgt tcatgtaatc ccgcttctca tcccatccgg ctcgggcgaa atccttgaag
    20581 cacgcacgac cggaaccgta aaccccacca ttcccgagcc agaatcatgt cccgcaaccc
    20641 tgaattgttc gaccgcgcct gccagaccat accaggcggc gtcaactcgc ccgtccgagc
    20701 ctttcgttcc gtgggcgggg cgcccagatt catccagcgg gcacaaggcc cttacatctg
    20761 ggacgtggac ggcgcccgtt acatcgacta catcgggtcg tggggcacgg cgatactggg
    20821 gcacgcgcat cccgaagtcg tacgcgcagt gcaggacgcc gccgtcaacg gtttgtcgtt
    20881 cggcaccccc accgaggccg agatcgagat cgccgagacc atcgcggcac gcattccatc
    20941 catcgaaaaa gtgcgattgg tgagctcggg cacggaagcg accatgaccg ccatccggct
    21001 ggcccggggc tacacggggc gctccaagat catcaagttc gagggctgtt accacggcca
    21061 tgccgacagc ctgctggtca aagcggggtc gggcatgctg actttcggca accccacatc
    21121 ggcgggcgtc ccgccggaat tcatccggca cacgctggtg ctggactaca accgcctgga
    21181 ctcggtcaaa gaagccttcc gccaacacgg cgcggacatc gcctgcgtga tcatcgaacc
    21241 aatggccggc aacatgaacc tggtcaaacc cgtgccgggc ttcctcgaag gcctgcgcca
    21301 gttgtgctcc gagcatggcg ccctgctcat cttcgatgag gtcatgacgg gtttccgtgt
    21361 cggcccgcaa ggcgtccagg ggctcacggg cgttcagccg gacttgacca cgctggcgaa
    21421 gatcatcggc ggcggcatgc cggtgggggc gtttggcggc cgggccgaca tcatggagca
    21481 catcgcgccc attgggtcgg tctatcaggc ggggacgctg tccggcaacc cggtggcggt
    21541 tgccgccggc atcaccacat tgcggctgct ggaggcgccg ggtttctatg aagacctgtc
    21601 ggtaggcacg gcccgcctca ccaacggcct ggccgaacgc gccaaggcgg ctggcgtctc
    21661 tttttgcgcc gattccgtcg ggggcatgtt cggcctgtat ttttcgtcga acattcccac
    21721 cacattcgat gaggtgtcac actgcaacat cgaacgcttc aacaagttct tccatggcat
    21781 gctgacgcgc ggcgtgcact tcgccccgtc cgccttcgag gcgggcttca tgtcggcggc
    21841 gcatggcgac gcggaaatca acgccaccct ggatgccgcg gaagccgtgt tcgcgaagct
    21901 ctgagaccag gccgcatccg acgacagccc tgaaaaacca tcgcctttct tctggatgga
    21961 aggcgatgcc tctcggttac ggcagccagt cagaaaatcg aaaaagcgag gctatcaagc
    22021 tggcgccggg ggcaccacgg ccgtgacctc gatctcgacg cgggcgcgat cttcgatcag
    22081 cgccgccacc tcgaccgccg tcatcgcggg gaaatggcgc ccgatgatct cgcggtaatg
    22141 ccggcccatc tcgcgcagcg atgccacgta ttcgtcgcgg tcacgcacgt accaggtcat
    22201 gcgcacgatg tgccggggct cggcaccgcc ctgggccaac acatccacga tgttgcgcaa
    22261 cgcctgcgcg acctgcccgg agaaatcatc cgtctcgaag acctgctggc cattccagcc
    22321 gatctggccg cccacgaaca gcaatctgcc acccaccatc agttccgtca tcacgccatt
    22381 ggcgtagccc ttggggggtg tccagtccgg cggctgcaag aattccatgt gtgtcatttc
    22441 ccagatttgg ttgaagaaga gtggcggcgg tgggcgtccg ctcaggacag gttgcgcaga
    22501 gcgtaccgct gcagctttcc ggtcccggtg cgcggcaagc tatcgacgaa gaccaccgcg
    22561 cgtggatact tgtatggcgc gatggtggcc ttgacgtggt cctgcaaggt tttgaccatc
    22621 gcgctgtcgc ccaggcagcc cggcttgagc accacgaatg ccttgacgat gttgccacgc
    22681 gcgggatccg ccacgccaac caccccacat tcggccaccg cctcatgcgt caacaatgcc
    22741 gcctccactt cgggcccggc gatgttgtag cccgccgaca cgatcatgtc gtcgttgcgg
    22801 gcctggtaaa acaaatagcc gtcttcatcc atgatgaacg tgtcacccgg aagattccag
    22861 ccttgctgga cgaaggcgcg ctgacggtca tcgtccaggt accggcatcc agttggccct
    22921 ttcaccgcca ggcgcccgat cgtgccacgc ggcacttctt ccagattctg gtcaacgacc
    22981 cgcaccacat accccggcac ggcgcggccg atggcaccgg cacgcacccg ctcattcgca
    23041 ctcgacacgt agacgtgaat caattcggtg ccccctatgc cgtcgatcat ttccactcct
    23101 gtggcgtcgc gccacagatg acgtgtgtca tcgggcagcg cctcgcccgc cgacaccgac
    23161 gcacgcaggg tggagaggtc gtggccgggc gccagtgccg ccatctgccg gtagaaagtc
    23221 ggggcggtga acgtcatggt cacaccgaaa tcctggaccg tggcgagcaa ttcggcgggg
    23281 gtgagctttt caaccaggac gaccgatgcg cccacgcgca gcggaaagca cagcaggcca
    23341 cccaggccga acgtgaacgc cagcggtggg gtgccgcaca ccacgtcgtt cggcatcatt
    23401 ttcaggatgt gccgggagaa cgtgtcgcac atggcgagaa cgtcatgatg gaagtgcatg
    23461 caccccttgg gcttgccggt ggtgccgctg gtgaacgcga tgaggcagac gtcgtcgaca
    23521 gcggtatcgc acgcggtgaa cccggccggc ttggcggccg ccagcgcctc gagcgagtcc
    23581 ggcccgtccc cgttgaaata ccgtacatgc gccaggtcgg cgcagtaatg ctcgtgcgaa
    23641 gagtcccgac aatgctccaa ctcgtcagcc aggcgcgcgt cgcacagcgc cgcactgatt
    23701 cgcgccttgt cgacgagctt cttcagttcg cccgaccgaa gcagcggcat ggagggcacc
    23761 gtgaccaacc ccgccttcac ggccgccagc cacgacgccg ccatcatcgg attgttcggc
    23821 ccgcgtagca ggatgcgatt gccgggtacc agccccatgt cttcggtgag cacatgcgcg
    23881 atgcggttgg tgagcgcggc gagttcgcca tacgtcatgg cgcgctgttc gccgttctcg
    23941 cgccaatgca gcgcgacgcg gtcggcgccc gcgcgcgcgg cgacgtcatc gaccagcgag
    24001 acggcgcaat tcaaccgcgc cggatacgcg acatccggat tgcccgcgat gagcatttcg
    24061 ggccattgat cgagtggcgg gagatgatcc cgcgcgaaag tatcgatatg ggctgaacgt
    24121 tccacgattt catgtctcct gatagaagtt cctctgccgc ctcacttctt attgaccaac
    24181 gaccttgagc atttcccggg cgatgatcag tttctgtacc tcggtggcac cttcgtagat
    24241 ccgcaacgcg cggatttcgc gataaagacg ctccacgggc gcatcactca cgacaccgcg
    24301 cgcaccgaac atctgcacgg cccggtcgat gacacgctgt gcggattccg tcgccgccat
    24361 cttggccatg gccgcctcac gcgtggtgcg cgctttcttg acgtcccgca gccacgccgc
    24421 gcggtacgtc agcagtgccg ccgcgtcgat ttcggtcgcc atgtcgccca gggcggactg
    24481 cgtgagttgc agatcggcga ggcgttggcc gaacatccgg cgcgagacac tgtgccgcaa
    24541 cgcctcgtgc agggcgcggc gcgcaaaacc caacgccgcg gccgccaccg acgcgcgaaa
    24601 gatatccagc gtcatcatgg ccagtttgaa accgtgcccg gcttcgccca ggcgctgcga
    24661 agccggaacg cggcattctt tgaattcgag cgtggccagt ggatgcggcg ccatgacgtc
    24721 gatgcgtcgc gggatggtca ggccgggggt gccggcatcg accacgaagg cgctgatgcc
    24781 gcgcgcgccg ggcgcttcac cagtgcgcgc gaacacgcaa tagaaatcgg cgatgccgcc
    24841 gttggaaatc caggttttct cgccgttcag cacatagccg ccctcgacgg cttccgcccg
    24901 acaactcatg gccgccacgt ccgaaccggc gtcgggctcg gacagggcga acgccgcgat
    24961 ggcattgccg gcggccacct cggggaggta gcgctcacgc agcgcgtcgc tccccccaag
    25021 ggagatggcg ccgctgccca gcccctgcat ggcgcaggcg aagtcggcca ggccatcgtg
    25081 aaacgccagc gtttcgcgca gcaggcagac ggcgcgcgag tcgatctccg gccaccgccc
    25141 gccccacttg ccggcggacc cgtggggcac gacataacgc agccagccgc cggcccccaa
    25201 cgcgcgaacc aggcggcggc aggtggcgtc ggtgtcggcg tgatccccct gcggcagatc
    25261 cgatgcgcac cacgccgcca ggctggttgc gagttcgcga tggcgccctt cgaagaaggg
    25321 ccattcaagc caagtcgtgt cgtccattca atccccttcg aaaacaggct tgcgcttggc
    25381 ggagaaggcc tcgtaggcgc ggaggaaatc cctggtctgc atgcagatgg cctgggcctg
    25441 ggcttcagcc tcgatggcgt cgtccaggct catggcccac tcctcgcgca gcagcgtttt
    25501 ggtcatggcg tgggcgaatg tcggcccgtc ggcgatctgc cgcgccatgg tctgcgcggc
    25561 ccgcaacagc tcgtcgggtt cgtgcaggct attgaagaag ccccaggcca gcccctcgtc
    25621 ggccgacatc gaccggccgg tgtacagcaa ctcggacgca cggccctggc cgatggtgcg
    25681 cggcaacaac gagcaggcgc ccatatccgc gccggcgagc cctacccggg tgaacagaaa
    25741 cgccgtgcgc gccgccgccg tgccgaaccg gaaatccgcc gccagcgcca ccatggcgcc
    25801 cgcaccggcg cagataccat cgaccgcggc aacgatgggc tgggggcagg ctcgcatggc
    25861 cttgaccaag tcacccgtca tgcgggtgaa atcaagcagt tgcggcatgt tcatcttcgt
    25921 gagcggcccg atgatctcgt gcacgtcacc accggagcag aaattgccgc cggctccggc
    25981 caccaccaca gtcttgatat ccgtggcgta cacgagggcg cgaaacagat cgcgcaactc
    26041 ggcataggaa tcgaacgtca aggggttctt gcgttccgga cggttcagcg tgatggtggc
    26101 gacttttccg tcatcggagg ccgaccagag aaaatgcctg gcctggtagc ccgccatcgc
    26161 gcgtttatgt ttcgccatgt tgtgtgcgac ttgccgcgtg tcttccatcg gattctcctt
    26221 gtcggttagc tcgttgccac ttccccgcca tcgacggcaa tggcctggcc gttgaccgag
    26281 gccgcccctg gcccgcacag ccaggccacc gtgtcggcca cttcgtcggg ttgcaccagc
    26341 cggcgttggg gattgcgctc ggccagtttt tccctggcct gctgttcggt catcccggtc
    26401 ttggcgatga tgttgcgcac cgcgtcgcgc accagatcgg tctcggtgta gccgggacac
    26461 acggcgttga ccgtgacacc ttttccggcc agctcccgcg ccagtgaacg cgtcaacccc
    26521 accacaccgt gcttggcggc gcaataggcg ctgacgtagg cgtagccgac cagtccagcg
    26581 gtgctggcca cattgacgat gcggccccgc tgtccggcaa gcatgtcagg cagcacagac
    26641 tggatgcaat ggaaggtgcc tgtcagattg acggcgatca tgtcccgcca ttgatcgtcc
    26701 gtcatccggt cgaatcgctc gctgatggcc tggccggcgt tgttgacgag cacgtccacc
    26761 ggcccgaatt cgcgccgcgc cgcgtccatg gcaccaccga cctggtcgtg gtggacaacg
    26821 tcggcctgaa cccgggccac gcgcccgggg gcgtcgagct cgcggacggc cgcatccaga
    26881 ggggcggcac cacgccccag cagcgtgacg cacaagcctc gtccaagcag gcgtcgcgcc
    26941 acggcgaggc cgattccacg cgcgccaccg gtcaccacgg cgtgacggaa tgcgggttgg
    27001 ttgctcattt gcctatctgc tgcgcgtagg cggcagcccg ttcaaagttg gtctcgagtt
    27061 ggcgcttggc cgggaagtac tgtttgggcc atgccacatc ggcgtagccg atgcgcgccg
    27121 attcccgcag cgtccaggcg gcatcggcga gatgcgggcg cgccagtgcg cacagatcgg
    27181 cccggcccga ggcgatgatg ctgttgacgt ggtcggcttc gaagatggcc cccaccgcaa
    27241 tcgtgggcac gttggcctcg ttgcggacgc ggtcggcgaa tggcgtctgg aacatgcgcc
    27301 catacactgg cttctcttcc ttgctgacct gccccgagga acaatccacc atgtcggcac
    27361 cagcggcctt gaaccggcgc gcgatctcca ccgcatcgtc ggcggtgatg ccaccctcca
    27421 cccagtcgtg cgccgaaatg cgcaccgaca tgggcaggtg cgcgggccag acttcgcgca
    27481 cggcctggaa cacttcaagg gggtagcgca ggcggttctc gagcgaacct ccataggcgt
    27541 cgacgcgcag gttggtcagc ggcgagatga agctcgacag cagatagccg tgtgcgcaat
    27601 gcagttcgag ccagtcgaag ccagctttcg cggcgcgccg cgcggcggcc acgaaccgct
    27661 cgagcacgtc gcgcatctgc tcatgcgtca tcccggccgg cacttgcgat acgccaggga
    27721 tgtatggaat gggcgaggcg gacaccaacg gccagttacc agagggcaga ggctgatccg
    27781 tgccttccca gctcacccgc gtggagcctt tgcgcccggc atggccaagc tgcatgccga
    27841 tgcgcgccgt gctgttttca tggacgaacc gcacgatgcg ggcaaacgcc tcttgttggg
    27901 tgtcattcca catgccgggg catgccgggg tgatgcgcgc atcgggcgag acacacgtca
    27961 tttcgaccat gaccagcccc gcgccgccca tggcgcgcgc gcccagatgc accagatgaa
    28021 agtcgcccgg cacgccatcg acgcacgaat acatggccat gggagaaacc atgatccggt
    28081 tcttcaaggt gatcccgcgc accgtgtagg gcaggtgcat gggcggaacc ggatgcgcgg
    28141 aaggcggggc ctggggttgc aggccatcgt tctggcgcgc cagcatccag cgctcgtaac
    28201 cctcgagcca ttcggcatcg cgcaggcgga gattttcgtg ggaaatgcgt tgcgagcggg
    28261 tcagcagcga ataggcgaac tgctccgggt cgaagttcgc gtagcgcgcg acgttctcga
    28321 accactcggt ggaattgcgc gcggcgttct gaatcttcag tacctcgacc gaacgcacat
    28381 cctggtaatg atctaggccg gcacgtaaat cgctgccctg gtcggtcagg cagcgatgca
    28441 gttctatggc gtcttccagc gccagtttcg tgcccgatcc gatggaaaaa tgcgcagtgt
    28501 gcgcggcatc gcccatcagc accaccggca cctcgcgtcc gtcgggcaac acctgccggt
    28561 gcacccaggt gtcgcagatg acgcgcggga agcggatcca gatggctgac ccgcgcaggt
    28621 gtgtggcgtt gcagaccagg cgatgcccgc ccagccaggg agcgaacagc ctctcgcaat
    28681 aggcgatgcc ctcttcctgg ctcatggttt ccagccccgc cgcacgccag gtttcgtccc
    28741 gggtctcgac gatgaacgtg gagagtccgg gttcgaactg gtaggcatgc gcctggaacc
    28801 agccgaattc ggtctgcacg aacgcgaaag tgaacgcatc gaacgtttgc tcggtgccga
    28861 gccacacgaa tcggcattgg cgcgtgtcga cgtcgggctt gtaggtgtcg gcgtaccgtt
    28921 tacgcaccct gctgttgagc ccgtcggagg cgatgaccag atcggcgcca tagcgcaagg
    28981 cgacctcctg atcgtcggaa acgtcggttt caaacacgag actcacgccc agttcctcgc
    29041 agcgcgcctg cagcacattg aggagcttct tgcgcccgat gccgatgaag ccgtgtccac
    29101 cgctgcgtat ggtgccgccc cggtagtgga tgtcgatgtc gtcccagcgg ttgaatgccg
    29161 agatgatgcg cccggccgac accggatcgg cctcggtgag attttccagc gtggcatcgg
    29221 agaacacgac gccccagccg aaagtgtcgt agggccggtt gcgctcgatg acgacgacct
    29281 cgtggcgcgc atcgctgagt ttcatcagca ggccgaagta cagccccgcc ggaccgccgc
    29341 ctatgcatac gattttcatg gatctggccc cgaaaattta ttttaggctt aaactatata
    29401 gtcaaaaaga aaatccggca agcattttcc cctcacactt ttgccgctat catgtgattg
    29461 aagattaaaa ttaacctgta tcaaccattt gtgacgcatc taccggattc gacgccatga
    29521 acgactttcc cgccgccagc atcgaacagg cgatccccga cctggaaagc cgcaccgtgc
    29581 ccgaggatca ccaggacctc cgcctgtggt tgcgcctgct gacctgcagc aacctgatag
    29641 aaggcacgat ccgcagccgc ctgcgcacgg aattcagcac cacgctgccc cgcttcgacc
    29701 tcatggcgca gttgctgcga gccccggatg gcatgaagat gagcgatctg tcgcagcaca
    29761 tgatggtcac caacggcaac atcaccggca tcaccgacca actcgagaaa gaggggctgg
    29821 tcgagcgggt gaaggtggcc acggatcgac gcagttcgtt gatccgcctc acgaccaagg
    29881 gcaaacgcac gttccgcaaa atggcgcagg cccatgaagc ctggatccag tcgctgttct
    29941 cagggctgtc cgacaccagt cgcaaggcct tgtacgatgc gctgggcgaa ctcaaactgg
    30001 tctcccgcac ccggcgctga cgtcagcgcc gctccccatt gggcgcggcg ccactgcgcg
    30061 ccaagcgccc caaatcccgc gtgaacccgg ttacactctt gtttttggcg ccgcaccatc
    30121 acctcgccct cgtggcgcgt cccttggtgc cacgcgtttg tcctcttttt cgccgagtgg
    30181 cacaccatgg ctgtcaacct gcacataccc aaggaatccg agatccacgc cgtgcccggt
    30241 atcgagatcg gcatcgctga agccggcatc cgcaaggccg gtcgacgcga cctgacggtc
    30301 ttcaggctgg cgcccggcac gaatgtggcc ggcgtgttca cgcgcaaccg cttccgtgcc
    30361 gcgccggtac aggtctgcga aaaacatctg gcaagtggcg cggcgatccg cgccctggtc
    30421 atcaacacgg gcaatgccaa cgccgggacc ggcgaaccgg gtctgagcgc ggcaaaccag
    30481 acctgcgcat cgctggccgg actgctcggg ctggaacccg ggcaagtgct gccgttttcc
    30541 accggcgtca tccttgaacc cttgccagtg gatcgtctgg tggcggcgct tcccgccgcc
    30601 gcggcgtcgt tgacggcttc caactggttc gcggccgccc acagcatcat gaccaccgac
    30661 acccagccca agatcgtgtc gcgtcgcgtc gaagtgggcg gcaagaccat caccatcacc
    30721 ggcgtcagca aaggggcggg catgatccgg cccaacatgg cgaccatgct ggggttcctg
    30781 gccacggacg cgggcctgcc aacagccttg ctgagcaagg cagccaaagc catcgccgac
    30841 cgttcattca atcgcatcac ggtcgatggc gatacgtcca ccaatgactc attcatcatc
    30901 atggccaccg gccagagcgg gctgaacatc gccggcgaat ccgacccggc ctatcctgcc
    30961 gtgttcgagg cattggcctc ggcggcgacc gaactcgccc agaagatcat ccgcgacgcc
    31021 gaaggggcga ccaagttcat gaccatccgc gtcgaacagg ccgccaacca ggaagaggcg
    31081 ttgaaggtcg cctacgcggt ggcccagtcc ccactcgtca aaaccgcgtt cttcgcgtcg
    31141 gatcccaacc tggggcgcat tctatgcgcg atcggctatg caggcatcga cgacctcgac
    31201 gtcacccgcc tcaacctgtg gctgggtgac gtattggtcg ccagcgcggg ggggcgccat
    31261 cccggctatc gggaagaaga cggccagcgc gtcatgaagg aagccgagat cgtcgtgcgc
    31321 atcgcgctgg gccgcggtga caccgccgag accgtctaca cctgcgattt ttcgcatgac
    31381 tacgtgtcga tcaacgccga ttatcggtcg tagaactggg gggttgtcag cccgacgaag
    31441 ggtcgcgcgg ggcactctcc ggccgcaacc ccttgacttg acgcggattt ttccgccatc
    31501 agcttgactt acccaatgag cacagactag aattgagggc ttggcgtttt gcgccaaggc
    31561 atccttattc ataccctagg gaaatcccac atgtacgcgg tcataaaaac cggtggcaag
    31621 caatatcgcg ttgccgctgg tcaaaaactc aagatagaac agataccggc tgacattggg
    31681 caagaaatca cgctcgacca ggtgttgtcg gtgggcgaag gcgaccagtt gcaaatcggt
    31741 acgcctttcg tcgccaacgc cgtggtcaag gctaccgttc ttgcgcaagg ccgtcacgac
    31801 aaggtccgta tcttcaagat gcgccggcgc aagcactacc agaagcgcca ggggcatcgc
    31861 cagaactaca ccgaagtccg catcgacgcg gtcgcggcct agtcgtcacc gaacccaccc
    31921 tttgcatagc aggagctaaa catggcacac aaaaaaggcg gcggctcaac acgcaacggc
    31981 cgcgaatcgg aatcgaaacg gctgggcgtg aaaacctatg gcggccaagc catttcagcg
    32041 ggcgcgatca tcgtgcgcca acgcggcacc cagttccatc cgggcaccaa tgttggcatc
    32101 ggcaaggatc acacgctgta ctcgctgatc aatggcaccg tcaaattcag cgtcaccggg
    32161 gcgctcaaca aacgcacggt gtcggtcttg gcgggcgaat aacccaccgg ccagccccat
    32221 atggggctcc caaagccccg cccctcaacg gcggggcttt tgttttagaa taaccgcagc
    32281 gcgtcggcac ccggcacgcg gtcttcgccc ggcatccagc tcgcgccgcg gcattctctt
    32341 tcccggtcca acatcatgaa attcgtagac gaggcaacca tcgaggtcgt cgcaggcaag
    32401 ggcggcaacg gcgtgtcgag ctttcgccgc gaaaagttca ttcccaaggg cggtcccgac
    32461 ggcggtgacg gcgggcgcgg cggcagcatc tatgccgtgg ccgaccgcaa tatcaacacc
    32521 cttatcgact atcgctatgc ccgcctgcac cgggccaaaa acggcgagaa cgggcgcgga
    32581 tcagaccaat acggcgccgg cgcgcccgac atcgtcctgc gcgtaccggt gggcaccatc
    32641 gtttacgacg ccgacaccgg gcaacagctt ttcgacctga accatcacga agacaaggtc
    32701 gtgctggtgg cgggcggcga aggcggctgg ggcaacatcc atttcaaatc cagcaccaac
    32761 cgcgcgcccc gtcagtggtc gccgggcaag gaaggcgaac agcgcaagct gcggctcgaa
    32821 ttgaaggtcc tggccgacgt cggcctgctg ggcatgccca atgccggcaa atccaccctc
    32881 atcaccaaga tttccaacgc gcgtccgaag atcgccgact acccgttcac gacactgcac
    32941 cccaatctgg gcgtggtgcg caccaccgcc tcgcgcagct tcgtggtcgc tgacattccc
    33001 ggtctcatca agggcgcatc cgaaggcgcc ggcctgggtc atctgttcct gcggcatctg
    33061 tcacgcaccc gggtactgct gcatctggtc gatgtggcgc cgctcgatcc agaggtcgat
    33121 cccgtcgagc acgccgctgc cgaggcgcgc gccatcgttg aagaactgcg tctttacgac
    33181 actgcgttgt acgagaagcc acgctggctc gttctcaaca agaccgacat ggtcgacgac
    33241 ccggctggcc tcgaagagcg gctgtgcaag gcactggact ggaccggccc agtgttccgc
    33301 atttcggcgc tgaatggcga aggcacccag gaactgacct gggaactgca ggactggctc
    33361 gatgccgaac gcgaaaaaga ctttctcgca caggcgcagg cccaggcaga ttatgccgac
    33421 cccgatcccc ggtttgacca gacccgagga gatgcctcca tcacaggcgc tccccacgac
    33481 cagtgatcca cgacaccagg gcctgttgac accctggttc gcaaccccta taccgcgata
    33541 catagtccgc ctcgtatcat gccttccgac tcgcccattt ccagcctcgt cgccgacgcg
    33601 cgacgcctcg tcgtcaaagt cggctcatcc ctggtcacca acgaaggcaa aggcatagat
    33661 cgtgccgccg tggcgtcatg ggcgtcgcag atcgccgacc tgcatcgcca gggacgccag
    33721 atggttctgg tgtccagcgg cgccatcgcc gaggggatgg tgcgcctggg ttggccgcaa
    33781 cgcccccata ccatgcatga actacaggcg gcggcggccg tgggccagat gggtctggtc
    33841 caggcctacg aagccgcatt cgccacgcac gccatccggg ccgcgcaaat cctgctcacc
    33901 catgaagacc tcgccgaccg gcgccgatac ctgaacgccc gcaccacgct ctacacgctc
    33961 ctgggcctgg gcgtggtgcc catcgtcaac gaaaacgata ccgtggtcac tgacgaaatc
    34021 cgcctgggcg acaacgacac gctgggcgcg ctggtgacca acctgatcga ggccgacgcc
    34081 ctcatcatcc tgaccgacca ggacggcctt tacaattccg acccccgcaa gaatcccgac
    34141 gcgcggctca ttcagaccgg tcaggcgggg gatccggcgc tcgagaccat ggccggcggc
    34201 gccggtagcg gcatcggcac cggcggcatg ttgaccaagg tgctggcggc gcggcgcgcg
    34261 gccaacagcg gcgggcacac catcatcgct tccgggcgcg agcccgacgt gctgctgcgt
    34321 ctcgccgcgg gcgaacccat aggcaccgaa ttgcgcgcac tcctgcctgt gcgctcggcg
    34381 cgcaagcgct ggctcgcgaa tcatctgcgg ctgcgcgggc gcgtcacgct ggatgcgggg
    34441 gcggtccagg ccttgacgca gaaccacaag agcctgctgg ccatcggcgt catcaaggtc
    34501 gaaggcgagt tcgagcgcgg cgacgtggtc gcctgcgtgg atcagaacgg cgtggaatgc
    34561 ggccgcgggc tgatcaacta ttcatcaggc gacacccgcc gcatcatgcg ccagcccagc
    34621 cggcgcattc ccgatatcct gggcagcatg tcagaccagg aactcatgca tcgggacaac
    34681 atggtcttcg tgcggccggg gcggcggccg gcttgatgcc tcggcatcac cgccagcgcc
    34741 aaagctgaac agtcgaatcg atcgggcaaa ttcgaatcaa ccaggcagca cactgcctct
    34801 gtcaccgggc agcacaccgg ctccgtcacc aggcggcaca ccggctccgt cggccgcgcc
    34861 cagcacgacg ggcccatcgc ttcgcaggct tgcctcccgg gccgccctca gggcaggcag
    34921 cctgtcctgc aggaaatcga tgaatacccg gaccttcgca ggaaccagac gcgatggaaa
    34981 cagggcgaac agcggcatcg gcccgaaatc ccagccgggc agaacacgca ccagccgctg
    35041 catcccgtca tcgcaccggg acagatattc agtaagctgc gcaatcccca ttccctgcag
    35101 acacaggcgg cgcagcattc ccgcgtcatt ggccgcgacg gccccggata ccggcaccag
    35161 ctcctgacgt ccctggcagc gcagccgcca gatcgcgtcc gtcttgccgg cggatcgccg
    35221 caggcacaca tggtgctcca gatcggcggg ctccagaggc accccccggc gtgccagata
    35281 atcgcgcgcg gcgtacaacc ccagttgcag atcatcgata tggcgcgaca ccacgccgga
    35341 gtccggctgc tcgcccaagc gcaacgccat gtcgtacggc tcggtcagca aatccacctg
    35401 gcgcatgctc aagtcgaact cgcattcgat ctccgggtat tgcgtacaaa aatcgcgcag
    35461 gatttcagac aacgacatca ctgccaggct ggtgggcacc gcgacccgca atcgcccctg
    35521 aggccgccgg gccgtctcga ccagttgttc gtgcgcgata cgcgctttgt cgatgatatt
    35581 ccggcagcgc tcgaagtaca cactgccggc ttcagtgagt tccacccggc gtgtgcttcg
    35641 atgaagcagc ttgacgccaa tgcatcgctc gagccctgaa attcggcgcg acagcgtcga
    35701 gaccggcacg cccgacgatt ccgcggcacg gctgaaattc ttgcgccgtg ccacctcgac
    35761 gaacagcgcc atgtcatcca gtgaggtatt cgttgatttt tccatattcg gtcattgcgg
    35821 actatcaacc atccgagcca attagcttac gcattcaatt attccaccaa taattggcta
    35881 tttcagtttt tattatttca tgtatgaaaa tatgatttgt gcaaatacgg gtttatacct
    35941 atctcaatca ggcagatact tctccacatc gacggtgagc accggcaagc cgcaagagcg
    36001 gttttccgtt atgtagcgtc gaccttcgaa acgaatggag ttcgcaatga aagcaatctt
    36061 aggtaccgca atgatttccc tgtccctgct ggccgctggc gcccaggccg ccgtatccga
    36121 tgtcgcaccg acccgcgcgc aagtcgtggc cgaactgcag caagcccgtt cggccgggct
    36181 gctgagcgag cacgaggcgg atccctaccc cgtgccggtg gtggccgcga gccataagag
    36241 ccgcgcggaa gtcctggcgg aactggcgca ggcgcgcagc gccggtcaac tcagcctgca
    36301 cgaggcggac ccgtacccgt cacccgcggc agacgcggac cctgtcgcct cgcgcgcaca
    36361 agtcctggcc gaactggcgc agtaccagtc ggcgcatccg cgggagttca ttgaacattg
    36421 atcccgcccg ccggtacggc tgaagaatcg accgcgccaa gacggcgggc aaggcccgcc
    36481 acgcatcacg gcccgatcag gcgacgacgc ctgaccgggc cgtctgcttc aagaggccct
    36541 gcgctttcgt agccgcttgc aactcaacga ggcgcgcctg caggaactcg atgaagacac
    36601 gcgtcttggc cggcatcagg cgcgagggaa acagcgccag cagaggcatc ggccggaact
    36661 cccattccgg cagcacacgc accagcggct cttcgccttc ctggcctcgc gacatgcggc
    36721 acgacagggg cacaatgcct agtccctgct ggcacatgcg ctggagcatg ccgacgttgt
    36781 tagccatgat ccggcccgcg accgcgacgg tctcgacgcg atcgccgcca tgcagttccc
    36841 agttggagtc ttccttgttg gccgtggccc tgaggcactg gtgatgcgca agctcggccg
    36901 gcaccacggg catgccgtga ttggcaatgt agcgacgcga cgcgaacaac cccagggaca
    36961 cgctcaccag gtggcgcgat accacccccg aatcaggctg ctggccaaag cgcaacacca
    37021 gatcgaacgg gtcggccagc aggtccaccg gatgaatgcc caagtcgaac tcgcactcga
    37081 tgtcagggta cagtgtgcag aactccccca ggaacccggc cagcgtgatc acggcaaagc
    37141 tggcgggcag cgatacccgc aggcgcccct tgggctgctg ggccacgtcg accagttgtt
    37201 catgcgcaat gcgcgcttca tcgacgatct tctggcatcg ctcgaagtag acgccgccgg
    37261 cctcggtgag ttccacccgg cgcgtgctgc ggttgaacag cttgaccccg atattgtgct
    37321 cgagctggtt gatacggcgc gacagggtcg agaccggcac ccccgacgat tccgaagcac
    37381 gactgaaatt ccggcgcttc gcgacctcga cgaacaaagc tatgtcatcc agagacgtat
    37441 ccatgcaatt ttcccggctg ccctattttt gcgcagcgat gaacagagtt tccttgacct
    37501 cttccatgac cacgcagctc ttggtctggg tcaccccggg caggcgcagc aggatgtcgc
    37561 ccaacaagtg gcggtactgg ccgatttcct tgatgcgagc cttgaccagg taatcgaaat
    37621 cgcccgacac caagtggcat tcctgaactt cgggaatgca caggatggcg cgccggaagt
    37681 cgtcgaacgt gcggtcggac ttgctggaca tggaaatttc gacaaacacc agcagcccgg
    37741 catcgagcgc gccgggcaac accctggcgt ggtatcccgt gatgatgccg tcgcgctcga
    37801 ggcgcttgac ccgctcggcg catggcgtca cggtcaggcc aacggcttcg gacaattcag
    37861 tcatggacat gcgaccgttg ttctgaagca gatcgaggat gtgccggtcc agtttgtcga
    37921 gcgtgctgac gggttgacgc tgtactctca attctcgccc ctcgggagac aaccaatatc
    37981 ccgattatag gattggcggg gctgagcatt cgcccggcca gcaataaaac ctgaatttcc
    38041 aaattcttca aaaaaaaaca ctattcggcc tatcttagaa tgccgccatg gcaacgctgg
    38101 gaaggtctgt cgtcgaagcg cgccataccg tcgggggcca tcagtttcgc cgaacaacat
    38161 aactcttatc ataaaagtgc agccatgaag gttttggttc taggcgccgg ggtcatcggg
    38221 gtgaccagcg cgtattttct cgccaggcaa ggtcatgacg tcaccgtcat cgatcgtgaa
    38281 tcggccgccg ccatggaaac cagctacggc aacgcgggcc aattgtcttt cggttactcg
    38341 tcaccatggg ccgcgccagg cgtcccgctg aaagccctga agtggatgtt cgccgagcac
    38401 ccgccgctca ccatccgccc ggacggctca ctgttccagt tgaagtggct gtttcagatg
    38461 tggcgcaact gcacggcggc gcgctatgcg atcaacaagg aacgcatggt gcgcatctcg
    38521 acatacagcc gtcaatgcct cgagcaactg cgcgccgaca ccggcattga attcgaaggc
    38581 cgctctcgcg ggacgctgca acttttccgc acacagcatc agctcgacgg gatctcgcaa
    38641 gatctcgccg tgctggccga agcgggcgta cgctacgacg tgctatcacg cgaagacttg
    38701 gtcaaagccg agccggcgct ggccaattcg ctgcacaagc tggcgggtgg cctgcggacg
    38761 ccggacgacg agaccggcga ctgccacctg ttcaccactc ggctggccgc tctggcgcgc
    38821 gaggcggggg tggacttccg ctatggcgtc gacatccgca aactgcgttt cggcgcgggt
    38881 gccatcgagg gcgtcgaatg ctccaccggg acaatgaccg ccgatgccta cgtggtggcg
    38941 ttggggtcgt actcccggct gatgctggac ggtaatctgt ccctgcccgt ctaccctctt
    39001 aaaggctatt ccatcacagt gcccatcacg aacgagacgg cggcacctgt ttccaccctg
    39061 ctggacgaaa cctacaagat cgcggtcacc cggttcgaca atcgtatccg ggtcggcggc
    39121 atggccgaaa tcgtgggcta cagcaaggcg ctcaacccca agcgacagaa gacgctggaa
    39181 atggtgctga atgatttatt cccgggcagc cacgcgcccg gcagcgcgca cttctggacg
    39241 ggcctgcggc ccaagacgcc ggacagcacg cccatcgtag gcaaaaccgc ctaccgcaac
    39301 ctcttcctga acacgggcca cggtacgctg ggctggacga tgtcatgcgg caccggccaa
    39361 ctgctggctg acatcgtttc aggacacgaa accgccatcc gcagcgacga cctgaacctc
    39421 agccgctatc gctgatctga cgcgccctgg gtgaacatgt aaccccagcc gtgcacggag
    39481 cgcagcggca acgccacgcc ccgatcctcg aattttcgcc gcatacggct caccagcaat
    39541 ccgatctgtc gaacgcgctg cggggcctcc gctccataag ccgcgttgac cgccgcagcc
    39601 aggtcgccgt gtgacgcccg cgaaccgggg gcatgcagca acgcgcgaag aaacgctctt
    39661 tctcccgtcg tcagggacac ccggcagtca tccggaccga ccagcaccca gtctcgttcc
    39721 tgcaatgacc aaccccggaa cggccagcgc ccggcagatt ggcgaatgcg acgaaacacc
    39781 gtcgacaata aagtggccag cagcgctgcc gacgcgcctt gcggacaaca cgcgtcagca
    39841 ccactgcgca gcgcttgtat caatgcgtct tcgccatcgg acggcaccat gaccacaatg
    39901 accaagtccg gatggcgcat gcgcacggaa gacgccgcca gacagttctg atccggcgcg
    39961 ccattgagga tcaggcacgc acccaccccg gcgtgggtaa tgaagtcggg caaccaccgt
    40021 ggatccgcgc aatggttgac caggaaacca cacgtctcga ggcgcgacgc catgtcggtg
    40081 ttttcatcca cgccaggcgg gccaggcgaa gcgggatcaa acaggatgac gatgaggttc
    40141 gatggcatga caaaagcgcg gaggaaacgg ttggggatga cctaggactc agggcggaag
    40201 aacgatcatg caaccagtcg gccgacgacg atgttctggt tttcgatcca aaatagcacg
    40261 aacgtgattt caattatcac caacgtgatt gaatttgatc atccaatcac gttgtgagca
    40321 aattcgccga ccgccttcgc caagcccgct tgttgcgggg attcacccag gcctcgctgg
    40381 cccaagcctg cggcctgtcg cagagcgcca tcgccaacta cgaaaccaag aaccgcaagg
    40441 cggcgaaaga cgtcttcaaa ctggccgacg tcctgcgtgt gaacgcggtc tggctcgggc
    40501 aaggggttgg acccatggaa acggccccga catcgttgcc atcaccctcc ggctccagaa
    40561 tcgccgaagc acacgtcggt cgacagcgcg accattggcc attcaacaat attccggcgg
    40621 accagttctg gacactgccc gagggtgacc gccagcttat cgaggatgcc gtcgcctcgc
    40681 tgatcgcgtc gttgagacga agccgataga aggcctggaa ctcgacgcaa tcctcgtggc
    40741 ccggcgggat cgggggaccg gggaacgcca cggaaaaacc gcgagacaca ggccggcgaa
    40801 aacctatata ctcaatgcca cgcaggggta tccgcgccgc caccaggcga tgtggtttga
    40861 gaaacaccct tcgtaccagt acggataatg ccgatgctgg gagcgtactc ggccattgcc
    40921 tctttcacgc cggggtcaat cccttctcgt cggctccaac cacattcttg gagcacacat
    40981 cttgaacgcc aatcctgatt ttctcgccag cagcgccacg gtagacgccg ccgcggtcga
    41041 gcccttgcca cgttcgcgca agatctacga aaccggctcg cgccccgacc tgcgtgttcc
    41101 gttccgggaa atttcccagg acgacacccc cacgatgtac ggcggcgagg ccaacccgcc
    41161 cctcacggtc tacgatgcca gcggccccta taccgatccg gaagccgtca tcgacatacg
    41221 cgccggcctg ccggcgctgc gcgccaactg gatcgccgaa cgcggcgaca ccgaggcgct
    41281 ggacggcccc accagccgat acggccagga ccgcctggcg aacaagcaac tcgacgcatt
    41341 gcgcttccag ctcaagcgca agcctgtgcg cgcacgcgat ggccgcaatg tgacgcagat
    41401 gcattacgcc cggcaaggca tcatcacgcc tgaaatggag ttcgtcgcga tccgcgagaa
    41461 cctgcgccgc gaacagtaca ttgaaagcct gcgcgccagc ggcccggagg gcgacaagct
    41521 ggcccgccga ctgctgcgcc agcatccggg acagtcattc ggtgccgcca cgcccacagc
    41581 catcacaccc gagttcgtgc gcgatgaaat cgcccggggc cgcgccatca ttccggcgaa
    41641 catcaatcac cctgaaaccg agcccatgat cattggccgc aatttcctgg tgaagatcaa
    41701 cgccaacatc ggcaactcgg cgctgggctc cagcatcagc gacgaggtcg agaaaatgac
    41761 ctgggccata cgctggggcg gcgacacggt catggatctt tccaccggca agaatattca
    41821 cgaaacgcgg gaatggatca tccgcaactc gcccgtgccc atcggcaccg tgcccatcta
    41881 ccaggcgctg gaaaaagtcg acggcaaggc ggccgagctg acctgggaaa tcttccgcga
    41941 caccttgatc gaacaagccg agcaaggggt ggactatttc accatccacg cgggggtgcg
    42001 cctgccgttt gtgcccatga cggccaatcg catgacgggc atcgtctcac gtggcggctc
    42061 catcatggca agctggtgtc tggcgcatca caaggaaagc ttcctgtacg agcgcttcga
    42121 agacatctgc gaaatcatga aggcctacga cgtgagcttc tcgctgggtg atggcctgcg
    42181 ccccgggtcg ggttacgacg ccaacgacga ggcccagttc gccgaactga agacgctggg
    42241 ggagctaacc caggtggcgt ggcgtcatga cgtgcaggtc atgatcgagg ggccgggcca
    42301 cgtgcccatg catctcatca aagagaacat ggacatgcaa ctcgagcact gtcacgaggc
    42361 accgttttat acgctggggc cgctgacgac cgacatcgcc cccggatacg accacatcac
    42421 gtcgggtatc ggggcggcgc tgatcggctg gtacggcacg gccatgctgt gctacgtgac
    42481 acccaaggaa cacctgggcc tgcctaacaa gaaagacgtg aaggacggca tcatcactta
    42541 caagatcgcc gcccacgccg ccgacttggc caaaggccat ccaggcgcgg ccgttcgcga
    42601 caacgcgctg tcgaaggcac gcttcgagtt ccgctgggac gaccagttca acctcggtct
    42661 cgatccggac accgcccggg acttccacga cgaaaccctg cccaagcaat ccatgaaagt
    42721 ggcgcacttc tgctcgatgt gcgggccgca tttctgcagt atgaaaatca cgcaggatgt
    42781 gcgcgactac gcccgcaagc aagggatcgg ggaatcggat gccctggccg aaggcatgcg
    42841 ggaaaaagcg ggggaattcc tggagaaagg cgccagcatc taccagcggg cgtgatcgac
    42901 tgccagtccg gcgccgctat tcgcggcgcc gggcgaggac accgtgtcag ctcttgaccg
    42961 cgtacaggta tagctctacg cggcgattca tggctcggcc agcggccgtg ctgttcgacg
    43021 cgatcgggtc gttgggccct cggccctcga tgctcagacg ggaaccggcc acgccctgac
    43081 tgctcaggta attcgtgacg gcttgcgccc ggttgagcga caaggtctgg ttgtaggccg
    43141 gcgacccggt gctgtctgtg tagccaatag ccttggcacg caattcggga tgctgcacga
    43201 gcgcccgggc cacgctgttg agtactggca gcagcgccgg cttcaagtca tacttattgg
    43261 tgtcgaacga cacattgctg gggatgttca ccttgagggt gccatcaggc atttcggtca
    43321 catccacgcc cagttgggag gcacctgact gctgcacgtc tttcttgatg cccgaccagt
    43381 tgtaacccgc aatcccgcca gccaccgcgc cgatgccggc accgatgagc gctccgtggc
    43441 cattgccgat caacgcgccg atacccgccc ccaacgccgc accgatacca gcgccagcgg
    43501 ccgtcttgcc gccttgtgac atctggggcg cgttagcgca cccaccaagc agacccgcca
    43561 cagccaaaca ggcaaccgcc tgactgattc gcattgttct catgttgttc tccttgtcag
    43621 ggcccaccgt tgaattccgg gccaatgaca atgctagcaa gctccgggag aaaaaattgt
    43681 tacattgtgg ttcccgagcc ggattcttga ccattcttga aggatttcca tccgtggcgg
    43741 ctcccgatcc gcccagccgt gggggtccaa aaggccgttg acgcgacagc gacgaaacgc
    43801 cagcacttac ccaaaccgcc gccgtaccgg ataatgccgg aaaccgcatg ggcggttacg
    43861 ttattattgg cgctgtcact tcatagtcac catgatcgaa ttccagcacg tattcaaatc
    43921 gtacggacgc gggcgcaaca tcctggccga tatcaatttc cgcatcgagc cgggcgaatt
    43981 catcttcgtc tccggcccat ccggcgctgg caaatcaacc ctgctcaaac tcatcggcgg
    44041 gctggagccg cccagccgcg ggcaggtcca cgtcaacggc catcgcatcg acaagatgtc
    44101 ggcgcgcgcg cgcccctatc tgcgccgcgc cgtgggcgtg attctgcaag acacgcacct
    44161 gctctatgac cgcaatgcca tccagaacat cctgttgccc ctgaccgtgg tcggcctgga
    44221 aagcggcgcc gccctgacgc gtgcgcgcgc cgccgtggaa aaagtcgggc tctccggcaa
    44281 ggaaaccctc aacccggtgg aaatgtccgg cggcgaacag cagcggctgg ccatcgccag
    44341 ggccattgtc aaccggccgg ccatcctgat cgccgacgaa cccaccgcca gcctggatca
    44401 ggatagcgca caccgcatca tggacgtatt ccgcgacttc aaccgggtgg gcgtgaccac
    44461 cctcatcgcc tcgcacgacc tgcgcctcat ggcctcgtac gccacccgca cattgcacat
    44521 cgatcaaagc ggccgtttct ccgatttcaa aggggtggcg gcatgaaccg ctggctcagg
    44581 caacaccgct atgcggccat ggccgccgtg cgccgcctgt tggcgcaccc attctcttcc
    44641 ctggccaacc tgctggtcat cgccctgaca ctggccatcc ccatcatcgc cgcgtccatt
    44701 ctggcctcgg cgcaacccgt ggccagacag atatcagtgt cgcccgagat caccttgttc
    44761 atgaagcccg gcgcgccggc cagcgccgcc acacaggccg ccgcgcgcat ccgcaagcaa
    44821 gctccgtccg cgatccgcga cgtgcgcatc gttccgcgtc agcaagccct ggcgcgcctc
    44881 aaagccgacc cggcgtgggc cgacgcgctg gccgccctgc cagacaaccc attgcccgac
    44941 gccgtgatcg tgaccctgga caacggtccc gatctggcgt cgcatgcggg caagctggcg
    45001 gaacaatgga aacaatggga tggcgtcggc cacgtccagc tcgacagtga ctgggtgcgc
    45061 cgactggaag ccatgctcgg ctttctgcgt gtcggcctgg cggtgctggc gttgggcgtg
    45121 gcgctggtgg tgctggccac ggtcttcaac accgtgcgcc tgcaggcgct gatgcagcgt
    45181 gaggaaatcg gcgctgcccg cctggtgggc gcgaccgaag cgttcgtgcg acgtccattc
    45241 ttgtatctgg gtgcactgac gggtgtcgtc gccagcgtgg tggccatcgg cctgtccgcc
    45301 atcgcgctgc atccgctcaa tgcggccctg gcacgcctgg gtcagagcta cggcacccaa
    45361 ttgtccctgc atctgccgag cagcgtgaac ctggtgctcg cggtcgtcgt ggtcgccatt
    45421 ctggccgcac tgtcggcgcg ctggtcggtc acgcgcaaca cccgtttctg aacatgctga
    45481 acccggtgcc ggataccgcg aacctcgcgt cgcgcatcgt ccgatggcaa cgacaatcgg
    45541 gccggcacga cctgccctgg caaggcacgc gcgacccgta ccgcatctgg ctctcggaga
    45601 tcatgctgca acagacgcag gtcaccactg tcatcggtta ctaccaacgg ttcctgcggc
    45661 gctttcccga tgtggcgacc ctggcggcat ccaccgagga cgaggtcatg ccctactggg
    45721 ccgggctggg ttactacgcc cgcgcgcgca accttcaccg ctgcgcccgc gttgtcgtga
    45781 cagactggaa tggcaagttc ccccccgacg caaccgagat cagccaactg ccaggcatcg
    45841 gacgatccac cgcggcggcc atcgccgcgt tcgcattcgg tgcccgacgc cccatcctgg
    45901 atggcaacgt caaacgcgtc ttcacgcgtt acttcggcat agaaggcgat cccggccaac
    45961 gcgccatcga aaccagccta tgggctctgg ccgaagacgt cgtcgcgaac gcgccgcccg
    46021 acctggacat gcccgcctac acgcaggggc tgatggatct tggcgccaca ttgtgcacgc
    46081 gcgcccgccc agggtgcgac aactgccctc tcgccacgga ttgctatgcc cggcgcaccg
    46141 cgcgacaggg tgattttccc aaccgaaaaa cccgtaaagc ccgccctgag cgtcattgcc
    46201 gaatgctggt tctggagagt ggcgggaaaa tactgctcga acgtcggccc gcacccggca
    46261 tctggggcgg cctgtggtgc ctgccgcagt atgacgagcc gggcgatctg acggtggcgc
    46321 tatcccgttg gggactggac aagagtcagg cccgccccct ggagccaatc aagcacgact
    46381 tcacgcattt ccgcctgcac atcgagccat ggctggccca cacggccaac gccgccctgg
    46441 ccgaacccgt cgccacgcaa aactgggtgg ccgccggcga cctggacgcc accgcccttc
    46501 ccgctcccgt caaaaaactg ttggcccggc ttttctagcg actttcggtc gtcgaaatgg
    46561 atgttgcatc gaacaccagc agtgctgcag attgaaccgt ttgagcggct ctggtcgacg
    46621 gaggttctat tctattaacc cgccccagga tggggggagt atgcatggac ggatattgct
    46681 ggcgttgtgg acgattgggc acttcgaggc ccacgtggca acttgatgcc taagcggtaa
    46741 ttcaagacat ccgcgacgtc attctctttg cccgaaacag gcttgccgac cccttcacat
    46801 gccatcaacg cgagctgtct atcggggcca ggggtgggcc cggtgtaagg cgtgcgccgg
    46861 atctggcttg gccgggcggg ggcagtcgtc gggcgactgt ttgagcggag tcgcgtcgga
    46921 acggcgcggc ggagcgagtt tcgcccggcg cccgcctgga cgagacagat tcggatgaag
    46981 cacgccgtgc cgggcacgcc cctggccccg atagacagcg tcaacgaaag aaagaataaa
    47041 cgacgccaaa gagataagac agcgccaacg aaataagcgg ttccaaagac agacagtccc
    47101 aaagaattcg ggccaccatc aatcagtgat ggcggcccga atcaggacta tttcaagcca
    47161 acttcagccg gcatggggac gagccctttt gccctggtgc ggcgtgggaa gccagctatt
    47221 accgcttcac gtcacgcacc acactacccg tgtacaactg gcgcggacgg ccgatcttgt
    47281 agttgggatc ggacagcatt tcgctccatt gggcaatcca gcccaccgtg cgggccagcg
    47341 cgaagatggc cgtgaacagg gacgtgggga tgccgatggc gcgctgcacg atgccggaat
    47401 agaagtcgac attgggatac agcttgcgtt cgacgaagta ctcgtcttcc agcgcgatgc
    47461 gttcgagttc catggccagc ttgaacaacg ggtctttttc caggcccagc gcagacagca
    47521 cctccttgca ggtctgctgc atgagcttgg cgcgcgggtc gtagttcttg tagacacgat
    47581 gaccaaagcc catgaggcgg acacccgagt tcttgtcctt gacctgctcc atgaactcgc
    47641 cgaccttctc gatgccgcca ttggcctgca ggtgttcgag catctgcagg caggcttcgt
    47701 tggcgccgcc atgcgccggt ccccacaggc aggccacccc ggccgcgatg gccgcgaacg
    47761 gattggtgcc cgacgagccg cacagccgca ccgtggaggt ggacgcgttc tgctcgtggt
    47821 cggcgtgcag aatgaagata cggtccagcg cgcgctcgac cacttcattc acgtgatatt
    47881 cctcgcaggg cgtggcgaac atcatgcgca ggaagttgcc ggtgtaggaa agatcgttct
    47941 tgggatagat atacggctgg cccaccgagt gcttgtacgc catggctacc aacgtgggca
    48001 tcttggcgat gagccgtatg gcggatacgt cgcgatgatg cgggttggtg atgtcggtgg
    48061 agtcgtggta gaaggccgac agcgccccga ccagacccgt gagcacagcc atggggtgtg
    48121 catcgcggcg gaagccgcgc agaaacacgt ggatctgctc gttgaccatg gtgtgatgcg
    48181 tgaccagcga gtcgaaatcc ttcttctgct gcccacccgg cagatcgcca ttcaggatga
    48241 ggtagcagat atccaggaag tcacatttct gggccagctg gtcgatgggg tagccacggt
    48301 acagcaattg gcctttgtcg ccgtcgatgt acgtgatgcc cgattcgcag gccgccgtgg
    48361 acaggaaccc cgggtcgtac gtgaacatgc ccgcctggct gtagagcttg cggatatcga
    48421 cgacatcggg ccctatggtt ccctgataga cggggaattc aatggagggg ctgccgtccg
    48481 aaaaagacag tgtggctttg ttgtctgata gttgcatcgc aattcctcaa aaaatcacag
    48541 cgcccgcatt tgtccgatca ggcgacggat gtcaggggtg tcgtaacacc cttgcggctc
    48601 cgccctggcc agcagtatgt cgagcaaatc gttatcaccc atctcgaaca acagagtcaa
    48661 cgcggagacg tcgacgtcgg tgaggtgggc ttcgtactga tccagaaatc gggtgagaat
    48721 caggtcgttt tccagcaaac cccgacgggc tctccagcgc aaacgcgcgc gatcgaggtc
    48781 ggacagtggc ttcattgaag acgcctcgtg tgatctacag gctgcggcgc gccagcatct
    48841 ctttgatctt gccgatggcg tgtgaggggt tcaacccctt cggacagacg tcggcgcagt
    48901 tcatgatggt gtggcaacgg aacagcctat agggatcgtc cagattgtct agacgctcgt
    48961 tggtggcctc atcgcgtgaa tcggcgatga agcggtaagc ctgcagcaac ccggccggcc
    49021 cgacgaattt gtcgggattc caccagaacg acgggcacga cgtggaacag caggcgcaca
    49081 gaatgcattc gtacaggccg ttgagctctt cgcgcgcttc cggcgtttgc agacgctcac
    49141 gctcgggcgg cggctgatcg ttgatcagga acggcttgat cgaatggtac tgattgtaga
    49201 agtcggtcat gtcgacgacc agatcgcgca ccaccggaag ccccggaagt gggcgcagca
    49261 cgatgggttc cttcagttcg agcagattgg tcgtgcacgc cagcccgttt ttgccattga
    49321 tgttcatggc atcggaacca cagaccccct cgcggcagga gcggcgcagt gtaaggctgt
    49381 cgtcgacatc gttcttgatg cgcaggatgg catcgagcag cattttgtcg ccgggatgca
    49441 gctcgacatc gagcttctgc atgtaggggc gctcgtcctt gtcgggatcg tagcggtaga
    49501 tttcaaactt cacgatgcgt ttttcactca tttcgatatc ctgcttagaa ggtccgggct
    49561 ttcggcggga agctgtcgac cgacagaggt ttcaattcaa cgggcttgta gtcgaggcga
    49621 cggccttcgg agaaccacag cgtgtgccgc agccattcct tgtcgtcgcg ttccgggtat
    49681 tccttgagtg agtgggcgcc acggctttcg tggcgactgg cggccgactt ggtcgtggcc
    49741 agcgcgacct cgatcatgtt ggtgagctcc agtgcctcgc aacgcgcggt attgaatacc
    49801 ttggacttgt ctttgaacga aacgttcgcc acgcgcgccg caagattttc gatttgctcg
    49861 actccctcat ttaggagatc ctgtgaacgg aacacaccgc aatggtgctg catggacaca
    49921 cggatggcgt tgccgacatc ggagaccgtt tcatcggact tgcggttctc cagcgcatcg
    49981 acgcgttcca gcgaatggtc gatggacgac tgtggaatcg gctgatgcat gtgctggcgc
    50041 tcgggatgcg aggccacgat gtggttgccg gccgcgcggc cgaacaccac caggtcgagt
    50101 aacgagttcg tgccgaggcg gttggcgccg tgcaccgata ccgcggcgca ttcgccaatg
    50161 gcgtacagac cgttgacaac cttgttttgg ccgttggcgt aattcagcac ctggccctgg
    50221 acgttggcgg gtatgccacc catctggtaa tggatggtcg gcaccaccgg aatgggttcc
    50281 ttgatggggt cgacgttggc gaacttgatg gcgatttcgc gaatggaggg caggcgtttc
    50341 ttgatggtgt ccgcgcccag atgatctagt ttcagcagga tgtggtcctt gttggcgccg
    50401 caaccccgcc cttccttgat ttcctggtcc atggaacggg acacgaagtc gcgtggcgcc
    50461 aagtctttga gcgtgggcgc gtagcgttcc atgaagcgct cgccatcctt gttgagcagg
    50521 atgccacctt cgccacgcac cccttcggtg atgagcaccc cggcacccgc cacgccagtg
    50581 gggtggaact gccagaactc catgtcttgc agaggcagat cggcgcgcgc cgccatgccc
    50641 aggccatcgc cggtattgat gtaggcattg gtggtggcgg cccagatacg gcccgcgccg
    50701 cccgtggcca gcaccgtggt cttggcctcg agcacgtaga tgtcgccggt ttccatttcc
    50761 agggcggtga cacccagaac atcgccggcc tcgttgcgca gcaggtcgag cgccatccat
    50821 tcgacgaaga actgggtgcg cgccgccacg ttgcgctgat agagcgtgtg caacagggcg
    50881 tggccggtgc ggtcagccgc ggcacaggcg cgctgcaccg gtttttcgcc gaaattggcg
    50941 gtgtggccgc caaaaggccg ttgataaatg gtgccgtcgg gattgcggtc gaacggcatg
    51001 ccgtagtgtt cgagttcgta gacggcgttg ggtgcttcgc ggcacatgaa ctcgatggcg
    51061 tcctggtcac cgagccagtc ggagcccttg acggtgtcgt acatgtgcca gtaccagttg
    51121 tcctggctca tgttggacag cgaggcgctg atgcctccct gcgccgccac cgtgtgcgaa
    51181 cgtgtgggaa acaccttgga gagcaccgcc accgacagcc ccgcctgggc caattgcagt
    51241 gaacagcgca tgccggcccc gccggcaccc acgacgacga catcgaatcg gcggtggggg
    51301 atggattttt tgacagcggc cacggtttac aagctccaga gaatatttag gaagtacacg
    51361 actgtgccca ccaaccatag aatggtcagg acatacagca acaggcgcac gcccgttggc
    51421 ttgacgtagt ccatccagat gtcgcgcaca ccgatccagg catgccacgc cagcgacagg
    51481 aacgccagcg aagccaggat ctgccccatg ggcagcccga tacagtggaa agagaacaga
    51541 tttcgccaac cggcgaaatc aaggttcgac gtgaagatga ggccaatgca gaggacgacg
    51601 gtgtagaccg ccatgatgac cgcggtgacg cgctggacga tgaaatcgcg tgcgccgtag
    51661 tgcgcgccca ccaggaggcg cttggtaccg atacgttctt gagtcgccat taccaggctc
    51721 cgaatagttt gaggccgaaa atcaccgtca gggccagact gaccccgaac accacgcccg
    51781 cggtcttctg cgccgtgatt ttgtcgtaac caagatgcag gtcgatggtg aggtagcgga
    51841 tgccggcgca gaaatggtgc aggaacgccc agatgaggat gagcagaatg atcttacaga
    51901 ctgggtgggc gacccatgcg tgcatggccg cgaagctttc aggcgacgtg acgctcagcg
    51961 cgaacaacgg cacgatcacc agaggcaggc aaagaaacag caacgcaccg ctgagacggt
    52021 gcatgatgga tgatttccct gccaacggca ggcggtagct cagtatctgc gagatgctga
    52081 tgttgcgata ctgcggacgc ggctttggcg cggaatcgga cataatggcc tcgaggtgat
    52141 gacaacaaat gatttattgc aaagcagtgc cggtattttc gcccaacagg cggtgtgtat
    52201 caaaccgaca cggaaaaaga cagctagttc agactgttcc ggtagtggaa ccgttccgtg
    52261 acatacaacc cccggcgcac ctcgaccggc cggtctccat aggtgtatga caaccgctcg
    52321 acgagcagca gcgggccgcc tggctcgact tcgagcagat cggcctgctc gaccgtggca
    52381 ggcaccgcct tgatcttttc atcggcgcgc accatgctga ttccgaattc ggattcgaac
    52441 agcgcataca gcggcccacg atagcgggcc agagattccg cggtaagccc ccgaaacacc
    52501 gaccctggca gccagatgtc gtcgaggatg gtgggaatct gatcaaacga caacaaccgc
    52561 cgcaaattca cggctgtgtc cgacgcacgc agctccagca ggcttgcgac gtcggcgggc
    52621 gccttgaggc ggcggcagtc gaggatgcga ctgcccgaca cgggcggttt gccgtcgtcg
    52681 ggggtgagac gcaggaaacg gtaccgtacc ttcgcctcgc tgtgcgtggc gacgaacgtg
    52741 ccctttccct ggcgccgcaa caacagattt tcggcggcga gttcgtcaat ggccttgcgc
    52801 accgtgccct ggctgacctg gaaccggctg gcaagttcga actcactggg gatcgcctca
    52861 ccgggcttcc attccccacg atccaggctt tgcaacaaca gccccttgat ctgctgatac
    52921 aacgggctga acgcggcgct gtgcgcgccg cgttcggggg cgttgggccg atccggcaga
    52981 ggggcgtcag gcatgggggg ggagactcgg gggacgagaa aaaacgacgg ttcgcatccc
    53041 atctattctc gcatgccagg ggatttctgt ctaacagtct tgtgtcttat ataagatata
    53101 agacaattga cgcaaacgga aatttgctct actctttcgc cgtcgcacag ggccatgatt
    53161 gcggcaccgc accaagacgc tcagccgagc gtttccagtg gtcccgtggt ggcctgacgt
    53221 cgtatcgtgg cgcctccggc atcctggttg acacgatgag ccaccgcccg cccaggtcgc
    53281 accaaacgca ttacactgct cgtacttgac attcaccccc tccaattcgc aaccttaccg
    53341 gagatcatcc atgtccaaac ccgccatgcg cgtcgccgtc accggcgccg cgggccagat
    53401 cggatacgca ctactgtttc gcatcgcctc gggcgaaatg ctcggcaaag accagcccgt
    53461 gattctccag cttctcgaga ttcccgacga aaaagcccag aacgcgctca agggcgtgat
    53521 gatggagctc gacgactgcg ccttcccgct gctggtcggc atgaccgcgc acagcgaccc
    53581 gcgcacggca ttcaaggacg ccgatgtcgc attgctggtg ggcgcgcgcc cacgcagcaa
    53641 aggcatggaa cgcaaggatc tcctgcaggt caacgcgcag atcttcaccg cccagggcaa
    53701 agcgctgaac gacgtcgcca gccgcaatgt caaggtgctg gtggtgggca atcccgccaa
    53761 caccaacgcc tatatcgcca tgaagtccgc gcccgatctg ccggcgaaga acttcaccgc
    53821 catgctgcgc ctggatcaca accgcgcgtt gtcgcaactc gcggccaaat ccggcaagcc
    53881 ggtgggcgac atcgaaaaac tcgtggtctg gggcaaccac tctcccacca tgtaccccga
    53941 catccgcttc gcgacggtcg gcgggcagag cctggccaaa ctcatcaacg acgacgcctg
    54001 gaaccgcgac accttcattc cgacggtggg caagcgaggc gcggccatca tcgaggcccg
    54061 cggcctgtcg tcggcggcct cggccgccaa tgccgccatc gatcacgttc acgactggct
    54121 gctcggcagc aacggcaagt gggtcaccat gggcgtgccg tccaacggat cgtacggtat
    54181 tccggaaggc atcgtgtacg gcgttcccgt caccaccgtc aacggcgagt acacgcgtgt
    54241 cgaaggcctg cagatagaca cattctcgcg cgagcgcatg gacttcacgc tgaaggaact
    54301 gctggaagag cgcgaaggcg tcaaggacct gctggcctga cggcccgccg cggcagttcg
    54361 ttccccgccc ggggcgcgcg gcggccaacc cgccgagcgc cccgcggaaa cccctcgccg
    54421 atccaggccg ctccggccca catccctggc cgacggaccg gcgaaccaac cgggcgggca
    54481 ccccgcccca cggagatccc gcaatgaccc gccccacttc cgccggcgcg ctgttccgcc
    54541 aggccctcgc ccaggaacaa ccgctgcaag tcatcggcgc catcaacgcc aatcatgcac
    54601 tgctggccaa gcgcgccggc taccgcgcca tctatctgtc tggcggtggc gtggccgccg
    54661 gctcgctggg cctgcccgat ctgggcatca acacgctaga tgacgtgctg accgacgtgc
    54721 gtcgcatcac cgacgtatgc gatgtgccgt tgatggtcga catcgacacg gggttcggtc
    54781 cctccgcgtt caatatcgcg cgcacggtca aaagcctgat caagttcggc gccgcggcct
    54841 gccacattga agaccaggtg ggcgccaagc gttgcggcca ccggccgggc aaggaaatcg
    54901 tgtccgcgcc cgaaatggtt gatcgcgtca aggcggcggt cgatgcccgc accgattccg
    54961 acttcttcat catcgcccgc accgacgcca tcgcggtcga tggggtggac gccgcgctgg
    55021 aacgggctgt cgcctgcgcc gaagcgggcg ccgacgccat cttcgccgaa gcctcgtacg
    55081 acctggacac ataccggcgt ttcaccaaaa cgctgaacgt gccggtgctg gccaacatca
    55141 cggaattcgg caagacgccg ctcttcaccg tggaagaact cgcgggcgcg ggcgtgggca
    55201 tcgtgctcta cccgttgtca gccttccgcg ccatgaacaa ggctgcggaa accgtctata
    55261 ccgccattcg gcgcgacgga caccagaaga atgtgatcga tatcatgcag acccgcgaag
    55321 agctctacga tcgcatcggg taccacgagt acgaatccag gctggacgca ctgtttcagc
    55381 aaggcaaatc caaataacct ttcgggagtt cagtgatggc aacaacaagc tctacagaaa
    55441 aagccgcgtt caaacccaag aaatccgtgg cgctgtccgg cgtcgtggcg ggcaataccg
    55501 cactgtgcac cgtcggccgc agcggcaacg acctgcacta ccgcggttat gacatcctgg
    55561 acatcgcgga aacgtgcgag ttcgaggaag tggcccatct gctcatccac ggcaagctgc
    55621 ccaacaaagc ggagctcgtc gcatacaagg gaaaactgcg ccgtttgcgc ggcctgccgg
    55681 ctcagttgca ggtggtgctg gaagccctgc ccgcggccag ccaccccatg gacgtcatgc
    55741 gcacggccgc gtcagtgctg ggctgcgtgc tgcctgagaa agacgatcac aacctgccgg
    55801 atgcgcgcga catcgccgac cgcctgatgg ccagcctggg ctcggcgctt ctgtactggt
    55861 accactacag tcacaacggc cgcgtcatcg acgtggaaac gtccgacgac agcattggcg
    55921 ggcacttcct gcatttgctg catggcgccg aaccctccga ggaatgggtg cgcgcgatgc
    55981 acacatcgct catcctgtac gcggagcacg aattcaacgc gtccaccttc gccagtcgcg
    56041 tcatcgcggg cacgggctcc gacatgtatt ccgcgatcgc tggcggcatc ggcgcgttgc
    56101 gcggccccaa gcacggcggc gccaacgagg tcgccttcga ggtgcagaaa cgctacgaga
    56161 cacccgacga cgccgaggcc gacatccgcc gccgcgtcga gaacaaggag gtcatcatcg
    56221 gtttcggcca tccggtctac acggtgtccg acccgcgcaa caagatcatc aaggacgtcg
    56281 cgcgcaagct gtcgaagaaa gcgcagaaca ccaagatgtt cgacgtggcg gaaaggctgg
    56341 aaacggtcat gtgggacatc aagaagatgt tccccaatct ggactggttc tccgccgtgt
    56401 cctaccacat gatgggcgtg cccacggcca tgttcacgcc gctgttcgtg atcgcgcgca
    56461 cggcgggctg gtcggcgcac atcatcgaac aacgcatcga caacaagatc atccgcccca
    56521 cggcgaacta cgtcggcccc gaggaccgga agtttgtcgc tctagagaaa cgcaagtaac
    56581 cccctgttcc aggaagaccc catgtccgcg ccaatttcca atatccgccc ggctcccgac
    56641 caggtgctgg tcgacatcgt cgattacgct ctcgattacc aaatcaagag cgaactcgcc
    56701 tacgagactg cccgcaactg ccttatcgac acgcttggct gcggcctcga agcgctggaa
    56761 tatccagcct gccgcaagct gctgggcccc atcgtgcccg gcaccattgt gccgaacggc
    56821 gccaaggtac ccggcacgca gttccagctc gacccggtcc aggccgcgtt caacatcggc
    56881 gccatcatcc gctggcttga tttcaacgat acctggctcg cggccgagtg gggccatccc
    56941 tccgacaacc tgggcggtat tctcgccacg gccgattggc tgtcgcgcaa tgccgtcgcc
    57001 gtcggcaagg cgccactcac gatgcgcgac gtgctcaccg gcatgatcaa ggcgcacgaa
    57061 atacagggct gcatcgcgct cgagaactcc ttcaacaagg tcggcctcga tcacgtggtc
    57121 ctggtcaagg tcgcaaccac ggccgtggtc tcgcagatgc tggggctcag ccgcgacgaa
    57181 accatcaacg ccgtgtccct cgcctgggtc gacgggcaaa gcctgcgcac ctaccgccac
    57241 gcgcccaaca caggtagccg caagagctgg gcggcgggcg acgccaccag tcgcgcggtg
    57301 cggctggccc tgatcgcccg taccggcgag atgggctacc cctcggtgtt gaccgccaag
    57361 acctggggct tctacgatgt gctgttcaaa ggccagccgt tcaagttcca gcgcccctat
    57421 ggcagctacg tgatggagaa cgtgctgttc aagatttcct tcccggcgga attccactcg
    57481 caaacagcgg tggaatgcgc catggaattg cacgagaaac tcaaggccgc gggcaagacc
    57541 accgaagaca tcaagaagat cacgatacgc acccacgagg cctgcatccg catcatcgac
    57601 aagaaagggc cgctgaacaa cccggctgac cgcgatcact gcgtgcagta catggtggcc
    57661 gtgccaatga tcttcgggcg cctgacggcg ggggactacg aagacagcat cgctgtcgat
    57721 ccgcgcatcg acgcgctgcg cgacaagatg atctgtgtcg aagacccggc gttcaccgcc
    57781 gactaccacg acccggaaaa gcgttccatc gccaacgcgc tcaccgtgga gttcaatgac
    57841 ggttcggcgt tcgacgaact tgtgtgtgaa tacccgatcg gccacaagcg tcgccgcaaa
    57901 gacggtattc cgttgctgga ggcgaagttc cgcaccaatc tggcgcggca gttcccagcc
    57961 cgccagcaac agcgcattct cggcgtatcg ctggatcaga agacgctcga ggccatgcca
    58021 gtgcatgaat acgtggatct gtacgtcatc tgagcctgca acctttcacc aacacctctt
    58081 caccgaccgt cttcggcgcc agcacgggcg cacccagcca cggcatgacg ccggagacac
    58141 ccccatcgcg gcgcaccgac accttttcag ccagtcaatg aatccttcct tcgaacacga
    58201 cgcggcccac caacgcatga cttgcctggt agacgacagc ctgtccgtcc tggagtacac
    58261 cctctcgggc tcgctgatga ccatcacgca cacaggggta cccacctcat tgcgcgggcg
    58321 gggtgtcgcg gcgggattga ccggcttcgc actggatatc gcgcgatgcc gcggctggcg
    58381 cgtgcgtccg ctgtgctcct acgccgacgc attcatccgg gcacaccccg agtaccagga
    58441 ccttctggcc tgacgggcgg caccaggcct gatgcgatga tgttccgatt ttttagtctt
    58501 atataagata taatacataa gacatataca ccccgtcacc cgtgtctcca cccgccgcgg
    58561 gcgtcggctt ttcaacactg ttccgaggtt tccctccatg ctcgaaaact accgccagca
    58621 cgtcgccgaa cgtgcagccc tgggcattcc cccgctgccg ctcaccgcgc aacagaccgc
    58681 cgacgtcatc gaattgctca agaacccgcc ggccggcgaa gaagcctttc tgctggatct
    58741 gatcacccat cgcgtgccgg cgggcgtcga cgacgccgcc aaggtcaagg cgtcgtatct
    58801 ggcggcgctg gcgctgggca aggaaacctg cgcgctcatc tctgcggaac aggccacgcg
    58861 gctgctgggc accatgctgg gcggctacaa catcagcccc ttgatcgaac tgctcgacca
    58921 ccccgccatg ggcccggtcg ccgccgaagg cctcaaacac actttgctgg ttttcgatgc
    58981 cttccacgac atcaaggaaa aggccgacaa gggcaatgcg aacgccaaag ccgtggtgca
    59041 aagctgggct gacgccgaat ggttcaccag ccgcccggaa gtgccggaaa gcctcacgat
    59101 cacagtgttc aaggtcacgg gggaaaccaa taccgacgac ttgtcacccg cccctgacgc
    59161 atggagccgg ccggatattc cgctgcacgc cctggccatg ctgaaaaacc cccgccccgg
    59221 catcgaaccg gaagagcccg gcaagaccgg cccgatgaag ttcctggaaa acctgaaaag
    59281 caaaggccac ctcgttgcct acgtgggcga cgtggtggga acgggctcgt cgcgcaaatc
    59341 ggcgaccaat tcggtcttgt ggctcacagg cgaagacatc cccttcgtgc ccaacaagcg
    59401 tttcggcggg gtgtgcctgg gcggcaaaat cgccccgatc ttctacaaca ccatggaaga
    59461 ctccggcgcg ctgcccatcg agctcgatgt ctcgagcatg aatatgggcg atgtggtcga
    59521 actgcgccca tatgaagggc gcgcactgaa gaatggcgcg gtgatcgccg aattccagct
    59581 caagtccgac gtgctgctcg acgaggcacg cgccggcgga cgcatcccgc tgatcattgg
    59641 ccgcgggctc accgcccggg cacgcgaagc cctgggcctg cctccgtcca cgctattccg
    59701 tctgcccaag gatccggcat catcgggtaa aggcttcacg ctcgcccaga agatggtggg
    59761 acgtgcatgc ggactacccg aaggccaggg cgtgcgtccc gacacctact gcgagccgcg
    59821 catgacctcg gtgggcagcc aggacaccac cggccccatg acccgggacg aactcaaaga
    59881 cctggcctgc ctgggctttt cggccgatct ggtgatgcag tcgttctgcc acacagccgc
    59941 ctaccccaag ccggtcgacg tcaagaccca tcacgaacta cccgcgttca tcagcacgcg
    60001 cggcggtgtc gcactgcgcc cgggcgacgg catcatccac tcctggctca accgcatgct
    60061 gctgccggat accgttggca cgggcggtga ctcgcacacc cgcttcccca tcggcatcag
    60121 ctttccggcc ggctcggggc tggtcgcctt cgcggccgca accggcgtga tgccgctgga
    60181 tatgccggaa tcggtgctgg tgcgcttcaa gggcactatg caacccggcg tcacgctgcg
    60241 cgacctggtc aacgccattc cgctctacgc catcaagcaa ggcctgctca ccgttgaaaa
    60301 acaaggcaag aagaacatct tctcgggccg catcatcgaa atcgagggcc tgcccgacct
    60361 gaagatcgaa caggctttcg aactgaccga cgcctcggcc gagcgttcgg cggcgggttg
    60421 caccgtgcgc ctgaacaaag agcccatcat cgaatatatc aacagcaaca tcacgctgct
    60481 gaagtggatg atcgccaacg gctacgaaga cgagcgcacc ctgggccgcc gcatcaaggc
    60541 catgcaggcc tggctggaca acccccagct gctggaaccc gacgccgacg ccgaatacgc
    60601 cgccgtcatc gagatcgacc tggcggacgt gcatgaaccc atcgtggcgt gcccgaacga
    60661 cccggacgac gtcaagacgc tgtccgaagt cgccggcacg gcgatcgatg aagtgttcat
    60721 cggcagttgc atgacgaaca tcggccattt ccgcgccgcg tcccgtcttc tggaaggcaa
    60781 gcgtgacatc ccgtcccgcc tgtgggtggc gccgcccacc aagatggacc agaagcagct
    60841 gaccgaggaa ggccattacg gaatcctggg cagtgccggg gcgcgcatgg agatgccggg
    60901 ctgctcgctg tgcatgggca accaggccca ggtgcgggaa ggcgccaccg tcatgtccac
    60961 gagcacgcgc aacttcccca accgcctggg aaagaacgcc aatgtgttcc tgggctcggc
    61021 ggagctcgcc gcgatctgtt cacgtctggg ccgcatcccg tcccgggagg aatacatggc
    61081 cgagatgggc gtgctgcagc gcgatagcga ccggatctac cgctacatga acttcgacca
    61141 gatcgaggat ttccgggaga tcgccgactc cgtgggggtg taggaacccc cgcataccgt
    61201 gcggactcat caccacacgg actccgaacc ggcctaaaat agcaggcgac attgccctcg
    61261 cgggcaccgg ccgcgcaccg ccgcggccga gtccgcttcg cgtcgacgca cgtttcacgg
    61321 ccatcaatcc cacggagtcc gtcatgccgc acaacacttt caacaccctc aagtcgttca
    61381 agatcggcgg aaaatccgcg aacttccatt cgctgcccga gctgggccga acgctgggtg
    61441 tcgatgtggc acgcctgccg gtgtcgctgc gcatcgtgct ggaatcggtg ctgcgcaact
    61501 gcgatggcca aaaggtcact gaaacccacg tccggcagct cgccaactgg aagcctcggg
    61561 ccaaacgctc cgaggaaatc ccattcgtgg tgtcgcgcgt cgtgctgcag gacttcaccg
    61621 gcgtgcccct gctcgccgac atcgccgcca tgcgctccgt cgcggcgcgc atgggcaaag
    61681 atccaaaacg catcgaaccc atggtgcccg tcgacctggt ggtcgaccac tcggtgatga
    61741 tcgacttctt cggcacccag cgcgcgctgg ggcagaacat ggccctggaa ttccagcgca
    61801 accaggaacg gtaccaattc ctgaaatggg gcatgcaggc gttcgacacg tttggcgtcg
    61861 tgccgccggg gttcggcatc gtccaccagg tcaacctcga acatctggcg cgcggcgtgc
    61921 accatgacaa ggccaacaac ctgtactacc cggatacatt ggtcggcacc gacagccaca
    61981 ccaccatgat caacggcatc ggcgtggtcg gctggggcgt gggcggcatc gaggccgaag
    62041 cgggcatgct gggccagccg gtctacatcc tcacgcccga cgtcgtcggc gtcgaactca
    62101 cgggcgaact acgcggcggc gtcacggcca ccgacctggt gctcaccgtg accgaactgc
    62161 tgcgcaagca gaaagtcgtc ggcaagttcg tcgagttctg tgggccgggc acctccagcc
    62221 tgtccgtcac cgaccgcgcc accatcggca acatgtcgcc cgaatatggc gccaccatgg
    62281 gctttttccc ggtcgacgac cgcaccatcg aatatttcaa gggcactggc cgcaccaagc
    62341 aggaaatcga tgcgctcaag gcgtatttca aggcgcaagg gctgtacggc atccccaacg
    62401 cgagggacat cgactactcc caggtcgtca cacttgatct ttccacggtg acaccctctc
    62461 tggccgggcc caagcgcccc caggatcgca tcgagctcgg caacgtgaaa cgcaagttca
    62521 ccgaactctt ctccgagcac tccaccaacg gtttcagcca gccccccgaa aagctcaacg
    62581 aagtcttcac caccagcgcg ggcacccagg tcaagaacgg cgacatcctc attgccgcca
    62641 tcacatcatg caccaacacc tccaacccca gcgtcctgct ggcggcgggc ctgatggcca
    62701 agaaggcggt cgaggcgggc ctgaagatac cgccgcacat caagacgtcg ctggcgccgg
    62761 gttcccgcgt tgtcacccat taccttgaaa agaccggtct gctgccctat ctgcaaaaac
    62821 tgggattcaa cgtggccgcc tacggctgca ccacctgcat cggcaacgcc ggcgacctca
    62881 ctccggatct gaacgaggcc atcaaccagc atgggctggt atgcgcggcc gtgctgtcgg
    62941 gcaaccgcaa cttcgaagcg cgcatccatc ccaacatcaa agccaatttc ctgggctccc
    63001 cgcctcttgt cctggcgtat gcgctggccg gaaccatgct gcgcgacctc atgaccgagc
    63061 cggtcggcaa gggccggcat ggcgacgtgt ggctgggcga catctggccg tccaatgccg
    63121 aagtccgggc gctgctcaac caggccctcg acccggaagc cttccgcgcc aattacggcg
    63181 aaatccgcac caaccccggc aaactctgga aggccatcga cggcgtcacg ggtgatgtct
    63241 acaactggcc aacatccact tacatcgccg agcccccgtt cttcgaaggc ttcggcatgc
    63301 ggccagcccc catgccagcc atcaacggcg cccatgccct ggccgtgttc ggcgactccg
    63361 tcaccaccga ccatatctcc cccgcgggct cgatcaagga atcctcgccg gccggccgct
    63421 ggctgcaggc caatggcgtc gccaaaaaag acttcaacag ctacggttcg cggcgcggca
    63481 atcacgaaat catgatgcgc ggcactttcg ccaacgtgcg catcaagaac ctgatgttgc
    63541 cgcccaatgc cgacggcagc cgctatgaag gcggtgaaac gctgcaccag cctgatggcg
    63601 agcgcttgtc catctacgac gccgccatgc gctacatcaa cgaaggcaag tccacggtcg
    63661 tgttcggcgg cgaagaatac ggcacggggt cctctcgcga ctgggcggcc aaaggcacac
    63721 agcttctggg cgtgcgcgcc gtgatcgccc gcagcttcga acgcatccac cgcagcaacc
    63781 tgatcggcat gggggtgttg cctctgcagt tccagggcaa tgattccgtc gattcgctgg
    63841 gtatctccgg cgacgaaacc tacgacatct cgggcatcga aaacggcatg aaaccccagc
    63901 aagaggtcac gctgaccata caccgcaaaa acggcgagtc catccgggtg cgcacgctgc
    63961 tgcgcatcga caccgacacc gaggtggact actaccagca cggtggcatc ctgccctacg
    64021 tcctgcgcga gttgctggcc gcatgatgcg gagccgggcg ggcggtccga acaccccgcc
    64081 ccccggcgta cggaaagtgt gttgcgcggc atccagcggt acactaagag gtttcacgac
    64141 gacagtgccc tcgcgcgcgg tcgccacgct tcactttgcc cacgaccatg ccccgaaccc
    64201 cgccttccag cccatcccgc ctgtcgcgcg acgcccagca cctgatttcc ctgacggagt
    64261 ccctggcact gtcaggatgc cgccttgagg acacctattg ggagaatctg cttggccagc
    64321 acctgaccaa gctgctgcgg gggcgccgaa acaaacacat cgagtccgtg ctcgagtacc
    64381 tgctggacaa caatctcaac gcatacgaga ttctggtcga gcaggcggaa accttttcgg
    64441 aatccatcac gctgtcggtc ggtgaccgtg actacgacgt gttgctcatc tcggcgccgc
    64501 tggtggcctg gacacgctat cagatcccgg gaggcgttct cgaggccaat gcgcaaaccg
    64561 ctctggcatc gaaactgctg gcgtgcgtgg cggcaccggg tgcccggctc gccctcatac
    64621 ccgaactggt cggcttcgaa caaatgcccc gcaccttcca ggaaacctgg gtatgggccc
    64681 atcgcctcgg gctggcggcg ctggatgcca aaaccgaacc ctgcgacatc cacaagacgg
    64741 aagactccga aggcatgctg gccgacgcgc gctttctcgt gggcgccatc gtggtgcccc
    64801 agggtgctcc gctgttccgc tggcaagccg acgcgcccga cacgccgaca cgcgaacaat
    64861 gcctgcaggc atggacccat gaatgcgccg agacactggg taccctgttc acgggatgta
    64921 cgctcgaata catgctgccc gacgcctact acgtgaacag ccgtgaagcc gatcgacgcg
    64981 tgcgcccgct ggcactcagg gccgcggtca catggctgca caccgcggtg gggcttccgc
    65041 catccgacgt ccgcgccaca atcgtgggct gcggcgattc ggtgcctgaa gaattccgtg
    65101 tcggtttcag cacccgccaa agcaacgacg tgatctacgg atgcgtctgg ccggtgttga
    65161 gcaaggaaga gtcgctgacc gatagttccg acactgccca ggccgacaca ccggatcaga
    65221 tcgccgccct gctcaaggag ctcggcgtgc aggatatccg ccgcttgcca ggcatccaac
    65281 ccgcggaata ctgtgacgac tgcggtgcgc cctattttcc caatccgctg ggcgaaatgc
    65341 agcacccgga aatccccgac gaggttgacc tcaatccggt ccactttcat tgatggcccg
    65401 ttgagccgga ttcgacacac agctcgatca agcgaaaccc ccgacaccgt gcccgcatcc
    65461 cccggcatcg atctgttctg ccgtgtcgtc gacaatttcg gcgacatcgg cgtctgctgg
    65521 cgtctggcgc ggcagctcgg caccctgacg ggcacgggcc acatccgcct ctgggtggac
    65581 gacttgggct cattcaaacg gattcagcca ggtgtggacg ctctggccga taaacaggtt
    65641 ctagctggcg tcgaaatcat ccattggacc gcccccgcgc ccgacctcgc gccccgggac
    65701 gttgtcatcg aggccttcgc ctgcgacccg cccgctacgt tcatccaacg catgaaacaa
    65761 gggcgtcacg cctggatcga cctggagtac ctcagtgccg aaccctggat agagagttgt
    65821 cacgcacggc cttccagaca gccggacggc atgatcaagt cgttcttttt ccccggattc
    65881 acaccagcca gcggtggact gctgcgcgaa gacggactgc tcgatcagcg cgatcgatgg
    65941 ctgtctgagc cgaacctccg ctggcgcctg ctgcgcgata tcgggctttc agcgtcacat
    66001 atttcgcaac tggaggcggg tggcttccag gcattcctgt tctgctatca gcacgcgccc
    66061 accggcgcct tgatccaggc tctggagcag ctcgggcgcc cgggcgtgat cattgttccg
    66121 gagggcgtgg cgcccgatct gcccggatcc ggcgctggca tgattcacgt gcagagaatc
    66181 ccgtttgtgg accaggatgg cttcgaccgc ctgctctgga gcagcgatct gaacctcgtg
    66241 cgcggcgagg attccctggt gcgcgccacg tgggcaggca aacccctgat ctggcacatc
    66301 tacccccagg aagaacaaac ccacatgacc aagctcgagg cctggctggg cttgtcaaac
    66361 tatccagcgc ggtccgccac caccatgcgc gcctggaaca gaggcgacgc cgacttggtt
    66421 cgtgaaaatc tgcaggccct gctgcaacca cccgcttggc gggagtggtg cgacgcctgc
    66481 ggccagcgag cacgggttct ggcgaaacac cccggcctcg ctcaatctct tctcgactac
    66541 tgcgcaaaac agctgcaaac aggttagaat accgggtttt gcacgtttac gctttgccaa
    66601 gccggctcgc acctttgctt ttggcgaagc ccattgcatg gagcactcca atgatgaaat
    66661 ccgcacaaga actgcgtgtt ggcaacgtgg tcaacgtcgg cagtgatgcc ctggtcgtcc
    66721 agaaggctga atacaacaaa tccgggcgca acgcggccgt ggtgaaactg aagttcaaga
    66781 acctgcttac gggttcgggc agtgagtcgg tcgtcaaggc cgacgagaaa ttcgatattg
    66841 tcgttctgga taagaaagaa tgcacgtatt cgtacttcgc cgaccccatg tatgtcttca
    66901 tggacgccga atacaaccag catgaagtcg aagcagacag catgggcgac gccctgaatt
    66961 acctcgagga aggcatgact gtggaagtcg tctttcacgc tggccgggcc atctcggtgg
    67021 acctccccac tatcgttgtg cgcgaaatca cctataccga acccgccacc cgtggtgaca
    67081 cctcgggcaa ggtgctgaag cccgccaaga cgaacaccgg catggaaatc agcgttccgt
    67141 tgttctgtga aatcggtgac aagatcgaaa tcgacacacg cacgggcgaa taccgcagcc
    67201 gcgtgatgtg aatcttcgtg gcggccctcg ccgccacggc ccctgccaga cccaaccagg
    67261 gcgggggcgg cacttggccg atgccaccta ctgcaggcgg tcatcgtcca ggaaatcggg
    67321 caccggcatc acggcgatgc catcccgggc cagttcctgg cgttcttctt ccgtcgcggt
    67381 gccgcgaatg gcgcgctctt gtgattcgcc ctcgtgcatg cgccgggcct cgtcggcaaa
    67441 gcgtgtgccg acgttttcgg cgccccgcac gatttcacgt atctggcgca gcatttccgc
    67501 ttgcaattgg gccagttgcc gccccgccgg cgcggccact ggctgagcct gcgcgggtgc
    67561 ttggcgaccg tccgcaggcg ttgaatcacg gcccgcgccg gtgttcacac gcgctgcggt
    67621 cagcttgcgc accacattgt ggctattgca gacagggcag gtcagcagac cgctttcacg
    67681 ttgcgattcg tagctgtcgg ccgagccgaa ccagccctcg aaaacgtggc cggaatcgca
    67741 ttggaggtcg aagactttca gtgacatacc ttcatgatgg ggccaaataa aaaaaaatca
    67801 atagcctttc ttcgctaccc cgccccgacg gtgactagca gtgcccctgt ttccagcagc
    67861 ggtagcgcca cacccaggga ggcaccggcg acgggtcgcg ccacaggcct tttccggcct
    67921 tgcgagcggc tgcctcgagc ccagggaggc tcggatcgcg catgtatttg ccacgcccag
    67981 ctccattggc ccacgccagc ccctgttcca cgagcacctg gttcgcagtg gatccgttgc
    68041 ccaggggcac gtcgcacaca ctgcgcccgt actggtcgtg ctcgaagcac gtcaggctca
    68101 gcaccctgcc cgccaccatg tcggccagcg cctgtctggc cacttgcgcc atgggctggc
    68161 cgggacgttc gcgatccttc gtggtctcgg gggcatcgat gcttgccagg cgaatccgct
    68221 cgcgacggcc atcgaccagc aacgtgaacg tatcgccgtc ggcgacatgc accaccttgc
    68281 cggtgagaga gtaggctgcc tcgcgagacg gcgcgtccat atgttgcccg gacgccacga
    68341 aaccc
//
LOCUS       DASTNK010000059         4506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_782401, whole genome shotgun sequence.
ACCESSION   DASTNK010000059 DASTNK010000000
VERSION     DASTNK010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782401"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="VFR20_04720"
     CDS             <1..1165
                     /locus_tag="VFR20_04720"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0229852.1"
                     /translation="DARGFGWEVGTPRHDWTALIAAKDKEIARLEGLYIQGLTSSGVE
                     LIRDRAVLVDPHTLRLKGSGRTLSARHILVATGGHAVRDETVEGIEHAVTSDDMFNLP
                     ALPKRLAIVGGGYIAVEFAGLMRALGSEVTIIHRREHLLRGFDDDARDVVEAAYRRRG
                     IALKLGYTLKRIEERPDGKLVTFMDGTQAAFDLVMVATGRKANITGLGLEAAGVATDN
                     GAIAVNAYSTTNAPSIHAVGDVTNRVNLTPVAIREGHALADTLFGDRPTAVDHHTVAT
                     AVFSTPEVGSVGYTESAAREAGKDVAIFMTTFRPIKATLSGREERTLMKLVVEKATDR
                     VLGVHVVGDSAAELIQVAAIALTCGATKTQFDATIAVHPTTAEELVTMRKPTR"
     gene            complement(1314..2618)
                     /locus_tag="VFR20_04725"
     CDS             complement(1314..2618)
                     /locus_tag="VFR20_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEU0229853.1"
                     /translation="MPATYSANANDILRVEHVRRGFAKSQDELLVLDDVNLSIRENEI
                     VGLLGRSGSGKSTLLRIIAGLIQPTGGSVKYEGEALEGPAKGVAMVFQTFALFPWLTV
                     LQNVEAGLEAQGVNQKERRERSLAAIDLIGMDGFENAYPRELSGGMRQRVGFARALVV
                     DPTLLLMDEPFSALDVLTAETLRTDLLDLWTQRQLKIKSVLIVTHNIEEAVFMCDRIL
                     VLSSNPGRVVAEIKVPFPHPRNRLHPAFRRLVDDIYAQMTSRVTTGGTTGGLELGTWL
                     PQVSTNLMAGLTETLAVAPYNGKADLPEIARTLHLEVDDLFPVAEMLQHLGFAEVREG
                     DIWLTPAGKAFADGDTQERKQMFARHLVRSVPMVARIHKVLNERPGHRAPRVRFEQEL
                     EDFLSDSAAEETLEAVINWGRYAEIFSYDDQAEMFSLEDADS"
     gene            complement(2746..4500)
                     /locus_tag="VFR20_04730"
     CDS             complement(2746..4500)
                     /locus_tag="VFR20_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006484435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0229854.1"
                     /translation="MRINVFIPSFNASAWRLLPNRWDFVAFPLILCVIAMGVVGYRQT
                     MAPMSVLATQAVSLSPSMLPEYALRTTLRMLAAMAASLVFTLVYGTLAAKSRRAEKIL
                     IPILDILQSVPVLGYVSFTVTFFLALFPGRVIGAECAAIFAIFTSQAWNMTFSFYQSL
                     CTVPRDLQEVSSNFQLTGWQRFWKLEVPFSMPGLIWNMMMSMSGGWFFVVASEAITVG
                     NKTITLPGIGSYLAMAIDQRNLESVGYVVLVMALVILAYDQLLFRPLVAWADKFRMEN
                     TASANAPDSWVLDLVRRTRLITQLIRPVSWGLATLARVPIRPPVSSMRFAGRSVVAAR
                     APSRWGDIAWTVFLLLLTAYLVVRVASYVHTGVGLPEVGHVFVLGLITLVRVMVLIAV
                     ASVIWVPVGVAIGLRPALAGKVQPIAQFLAAFPANLLFPVFVVVIVKYHLNADVWLSP
                     LIILGTQWYILFNVIAGTTAFPNDFKEAAANFRIRGWQWWRKIMLPGIFPYYLTGAIT
                     ASGGAWNASIVAEYVSWGNDKLVAHGLGAYIAQNTIAGDFPKIILGVAVMSLFVTLFN
                     RLLWRPLYAYANSRLRMD"
BASE COUNT          935 a         1512 c         1372 g          687 t
ORIGIN      
        1 cgacgcccgc gggttcggct gggaggtcgg cacgccccgg cacgactgga ccgcgctgat
       61 cgcggccaag gacaaggaaa tcgcccgcct cgagggcctg tacatccagg ggctgaccag
      121 cagcggcgtc gagctgatcc gcgaccgcgc cgtgctcgtc gatccgcaca ccctgcgcct
      181 gaaagggtcg ggccgcacgc tcagcgcccg ccacatcctg gtcgcgaccg gcggccacgc
      241 cgtccgggac gaaacggtag aaggcatcga gcacgccgtc acatccgacg acatgttcaa
      301 cctgcccgcg ctcccgaagc gcctggccat cgtggggggt ggctacatcg ccgtggagtt
      361 cgccgggttg atgcgcgcgc tgggcagcga ggtcaccatc attcaccggc gcgaacacct
      421 gctgcgcggc ttcgacgacg atgcgcgcga cgtggtcgag gccgcctacc gccggcgcgg
      481 catcgcactg aaactgggtt acacgctcaa gcgcatcgag gaaaggcccg acggcaagct
      541 ggtcacgttc atggatggca cccaggcggc attcgatctg gtcatggtcg ccaccgggcg
      601 caaggccaac atcaccggac tggggctgga agccgcgggc gtcgccacag acaacggcgc
      661 catcgcggtc aacgcctatt caacaaccaa tgcgccgtcc atccacgcgg tgggcgatgt
      721 cacaaaccgg gtgaatctca cgccggtcgc catccgcgag ggccacgcac tggccgacac
      781 cttgttcggc gaccgcccca ccgccgtcga tcatcacacc gtcgccacgg ccgtgttctc
      841 aaccccggaa gtcggcagcg tgggctatac cgaatcagcg gcgcgcgagg ccggcaagga
      901 cgtcgccatc ttcatgacca cgttccggcc gatcaaggcc accttgtcgg ggcgcgaaga
      961 gcgcacactc atgaagctcg tggtcgagaa ggccaccgat cgagtcctgg gggtgcatgt
     1021 cgtgggcgac agcgcggcgg aactcatcca ggttgccgcc atcgccctca cctgtggcgc
     1081 gaccaagacc cagttcgacg ccaccatcgc ggtgcatccc accacggcgg aagagctggt
     1141 cacgatgcgc aaaccgacgc gttgaagtgc ctcgagcccc cgcccagatt ccgtgcgcgt
     1201 cgaatcggag tctctcgaaa gcggcgcgat ccgcaaggta gaagcggtgc caaagagatt
     1261 ttcgagatca gccctgaaac caaacgggcg gggctgcctg ggggcctgcc gacctacgaa
     1321 tcggcgtctt ccagactgaa catttcagct tggtcgtcat acgagaaaat ttccgcgtag
     1381 cggccccagt tgataacggc ctccagggtc tcttcggcgg cgctgtcgga caggaagtcc
     1441 tcgagctcct gctcgaagcg cacccggggc gccctgtgcc cggggcgttc gttgagcacc
     1501 ttgtggatgc gggccaccat gggcaccgag cgcaccaggt ggcgggcgaa catctgcttg
     1561 cgctcctggg tgtcgccatc ggcgaacgcc ttgcccgccg gggtgagcca gatgtccccc
     1621 tccctgacct ccgcgaaccc aaggtgctgc agcatttcgg cgacggggaa caagtcgtcg
     1681 acctccagat gcagggtgcg cgcgatttcg gggagatccg ccttgccgtt gtaaggcgcc
     1741 accgccagcg tctcggtgag gccggccatc aggttggtcg acacctgggg cagccaggtg
     1801 cccagctcca gcccgccggt cgtgccgccg gtcgtgaccc gggaggtcat ttgagcgtag
     1861 atgtcatcga ccagacggcg gaacgccggg tggagccggt tgcggggatg cggaaacggc
     1921 accttgatct cggcgaccac ccgacccgga ttggacgaca ggaccagaat ccgatcacac
     1981 atgaacacgg cttcctcgat gttgtgcgtg acgatcaaca ccgacttgat cttcagttgc
     2041 cgctgagtcc agaggtcaag cagatcggtg cgcagcgtct cggccgtcag gacatccagg
     2101 gcggaaaacg gctcgtccat caacaacaac gtggggtcga ccaccagcgc gcgggcgaaa
     2161 cccacacgct gccgcatgcc gcccgacagt tcgcgcggat aggcattctc gaaaccgtcc
     2221 atgccgatca ggtcaatggc cgcgagagaa cgctcgcggc gttccttttg attgacaccc
     2281 tgggcctcca gcccggcctc gacgttctgc aggaccgtga gccacggaaa cagcgcaaac
     2341 gtctggaaga ccattgcaac gcccttggcc ggtccttcca acgcctcccc ctcgtacttc
     2401 accgagccac ccgttggctg gatcaagccg gcgatgatgc gcaacaacgt cgactttccc
     2461 gaccccgaac gccctagcag accgacgatc tcgttctcgc gtattgagag attcacatcg
     2521 tcgagcacca gcagttcgtc ctgcgatttg gcaaagccgc gtcgcacatg ttcgacacgc
     2581 aggatgtcat tggcgttggc gctgtacgtg gctggcatgg cggtctcttt caaaaatcat
     2641 tcgaacaaac agaaaacaca acaatgaggg cttctgtgca aatcatcacg cctcatgcaa
     2701 tcgaggcgta ccgcgcgatc ggcccagagt tccctcgcgc cggattcaat ccatgcgcag
     2761 cctgctgttg gcataggcgt acagagggcg ccagagcagc cggttgaaca aggtcacgaa
     2821 aagcgacatc acggccacgc caaggatgat tttcggaaaa tccccggcga tcgtgttctg
     2881 cgcaatgtaa gcgcccaggc cgtgcgccac cagcttgtcg ttgccccatg acacatattc
     2941 cgccacgata ctggcgttcc aggccccgcc cgacgccgtg atcgctccgg tcaggtaata
     3001 ggggaaaatc cccggaagca tgattttccg ccaccactgc caaccccgga tccggaagtt
     3061 ggccgccgct tccttgaaat cgttgggaaa cgccgtggtt cccgcaatga cgttgaacag
     3121 tatgtaccat tgcgtcccga ggatgatgag cggcgacagc cagacatcgg cgttcaggtg
     3181 atacttcacg atcaccacga cgaagaccgg aaacagaaga ttggccggga acgccgccag
     3241 gaactgcgcg attggctgga ctttcccggc cagcgccggc cgcaagccta tcgccacgcc
     3301 cacaggaacc cagatcaccg acgccacggc gatgagcacc atcacgcgaa ccagcgtaat
     3361 cagacccagc acgaacacgt gccccacttc cggcaagccc acgccggtgt gcacgtacga
     3421 ggccacccgc accaccagat acgccgtcag cagcagcagg aacaccgtcc aggcgatgtc
     3481 cccccatcgg gacggcgcgc gcgccgccac cacgctccgc cccgcgaagc gcatcgagga
     3541 aaccggcggg cggatgggga cgcgggccag ggtcgccaga ccccagctca cgggccggat
     3601 caactgggtg atcaggcgag tgcggcgcac cagatccagc acccaggaat ccggcgcgtt
     3661 ggccgaggcg gtgttctcca tgcggaactt gtcggcccac gccaccagcg ggcggaacag
     3721 caactggtca taggccagga tcaccagcgc catgaccagc accacgtagc ccacgctctc
     3781 gagattgcgc tggtcgatgg ccatggccag ataggaacca atgcccggca gggtgatcgt
     3841 cttgttgccc accgtgatgg cttccgacgc caccacgaag aaccagccgc ccgacatgga
     3901 catcatcatg ttccagatca ggcccggcat cgaaaacggc acctccagct tccagaaacg
     3961 ctgccagccg gtgagctgga aattggacga cacctcctgc agatcgcgtg gcaccgtgca
     4021 gagcgactgg tagaagctga acgtcatgtt ccacgcctgg ctggtgaaga tcgcgaagat
     4081 ggccgcgcac tcagccccga tcacccgtcc cgggaacaac gccagaaaga acgtcaccgt
     4141 gaacgacaca tagcccagca ccggcaccga ctgcagaatg tccagaatgg ggatcagtat
     4201 cttctcggcg cgccggctct tggccgccaa tgtgccgtac acgagcgtga acaccagcga
     4261 ggcggccatg gccgccagca tgcgcagcgt ggtgcgcaac gcgtactcgg gcaacatcga
     4321 aggcgaaagc gacaccgcct gggtcgcgag caccgacatc ggcgccatgg tctggcgata
     4381 cccgaccacc cccatggcga tcacgcaaag aatcagcggg aacgcgacga aatcccaacg
     4441 attgggaagc aatcgccacg cggaggcatt gaacgagggg atgaaaacat taatacgcat
     4501 gcgacg
//
LOCUS       DASTNK010000060         8238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1153984, whole genome shotgun sequence.
ACCESSION   DASTNK010000060 DASTNK010000000
VERSION     DASTNK010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8238
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153984"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="VFR20_04735"
     CDS             complement(<1..1197)
                     /locus_tag="VFR20_04735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0229855.1"
                     /translation="MVNLGVQPIKAYFQAGITSMQWVVDSYNLVYAALLLSGGLIADL
                     WGRKRAYLTGVLLFAGASIVCGMASSVGVLIAGRVAAGVGAALLTPATLAILRVGWPD
                     PVRRGRALGIWAACNGLAFAIAPTLGGLLISWFGWRSLFFVAIPVCGLALALAWRSIT
                     ESADPADRHADLTGQVLGALTLGGLTLAAIEARGHGVLSLAALIIGAAALALFSRAEA
                     RKGAAAMVPLELFRIPDMRGAIIATVGMTSGMYGVLFLQPLVWLEMGAMTATQAGLAL
                     MPMALVFLVISPLTGRLSQTFGAHQPACGGVLIIGIGLLTIGWGAGHPAIALSEAGLT
                     LTGVGMGFATGPLMGLAVGSAPAARSGTASALINVARIAGAALGVAVLGTLYATRHDP
                     SSGLRLA"
     gene            complement(1210..1359)
                     /locus_tag="VFR20_04740"
     CDS             complement(1210..1359)
                     /locus_tag="VFR20_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229856.1"
                     /translation="MTQSSITSLLPDTEPHDPGTSSSSREALSGATAGTSTALTALCL
                     AVLIA"
     gene            1783..2463
                     /locus_tag="VFR20_04745"
     CDS             1783..2463
                     /locus_tag="VFR20_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0229857.1"
                     /translation="METFADFSSVAYLLADPSRAIMLAALLDGRALPAGELAHAAGIT
                     AQTASAHLSKLLEGGLLACESEGRHRYYRLAGPDVAQALEHLAVLSGSVRRKPLGTEG
                     TTLRQARCCYDHLAGRLGVVITQSLVGHAYIVPIDGKQYEITSDGMEWFESLGIKGLQ
                     PSRRGLARQCLDWTERAHHVAGPLGSGLLRALCDLGWLRRSRSSRLVQVTPEGHAGLK
                     AQLGVDVS"
     gene            2553..2771
                     /locus_tag="VFR20_04750"
     CDS             2553..2771
                     /locus_tag="VFR20_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229858.1"
                     /translation="MLTDGAPDWADAPEGWDWLAQDEDGRWFWYGVRPQPSLGGGVWR
                     APSKAQRLALHGTPNPDWVDSLHHRPAF"
     gene            complement(2929..3426)
                     /locus_tag="VFR20_04755"
     CDS             complement(2929..3426)
                     /locus_tag="VFR20_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229859.1"
                     /translation="MRPLIPTLLSTALALCSLVAASAAQCAEMIWPVPPSRLSLDTPY
                     GALSVKESGYVYESFLQFDDKPVQPRIEGIVNITYAFATPTSQVALVSVNKGSYQCPF
                     TYRWITVDEKGYTVSPSFGSCSKQIKVFATGRKLTMETPNAQKPDEVDVYVYDGKTIQ
                     HHTKR"
     gene            complement(3588..3788)
                     /locus_tag="VFR20_04760"
     CDS             complement(3588..3788)
                     /locus_tag="VFR20_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229860.1"
                     /translation="MKRGAILLTLLAMAFVWWWLSRGPFATKALTLQARLRNIGKSLV
                     AGVIAYFCVMLLMLIYMAITGF"
     gene            complement(3828..4214)
                     /locus_tag="VFR20_04765"
     CDS             complement(3828..4214)
                     /locus_tag="VFR20_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HEU0229861.1"
                     /translation="MSLAAICLMAGALSVKVPADHFTLVWRHSIEKIDWSEDYAVQGN
                     TLLLKAAHVQGDGAGMEPPPDSTFDPKHAYWTYHPNIPPLPRLNLARSRFTQDYRFCV
                     NGACRPMADLVPISAGGSLIIEPCSR"
     gene            complement(4255..6354)
                     /locus_tag="VFR20_04770"
     CDS             complement(4255..6354)
                     /locus_tag="VFR20_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HEU0229862.1"
                     /translation="MVSHASMHDANLGNPVPEVEVDELGHQRSLTGKAALVFAAFALV
                     FSFYQLIVAAFSPLSSQVIRSLHVGFLMMLAFMIFPMTKAAVGKKIPWYDWGLALTGF
                     ALGFYHWYFETDLILRAGSPTVVDLVVGSVVLLLLFESCRRILGLALPLICGAFLLYG
                     MFGEYLPGALAHRPYYFDQIISQMYLGVEGIYGLPTLVSATYIFLFILFGSFLEQAGM
                     IRLFNNLALGFVGHSRGGPAKVAVISSGFMGTISGSGVANVLTVGQFTIPLMKRFGYS
                     SVFAGAVEATASMGGQIMPPVMGAVAFIMAETLNVPYLEICKAAVIPAVLYYVTAFWM
                     VHLEAGRAGLLGLPKEDCPNPWKSMKEYWYLLLPLGVLVFMLFDGFTPMFAGLTGLGL
                     TATLILGANITASISPMAFRFFFWIVIGLAAADFFKHGIWVIIGVIAALAISCLFVKG
                     GKQTLTLIRDGLINGAKQALPVGLACAIVGIIIGILTLTGAATSFAGFILEVGQKSLF
                     LSLLLTMVVCLILGMGIPTIPNYIITSSIAAPALLKLGVPLIVSHMFVFYFGIMADLT
                     PPVALAALAASSIAKASFMRIAIRATQIAIAGFVIPYMAVYDPSLMMQGGAWYDTAYM
                     VFKALVAITLWGGASVGFLLAPLSWPQRALSAVASLSLVVASVWTDQVGFGLAILLLL
                     WHWPAARQHKRLQRQPA"
     gene            complement(6454..7428)
                     /locus_tag="VFR20_04775"
     CDS             complement(6454..7428)
                     /locus_tag="VFR20_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018025402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEU0229863.1"
                     /translation="MKFSLSVSLNKVLLGACAATLVAAAPVAHAANYLNIATGGTSGV
                     YYPMGVALSQVYSKAMPDVKVSVQSTKASVENLNLLQAGRAELAFTLADSLSDAWKGN
                     AEVGFTKKLDNLRAIAGIYSNYIQIVARADSGIKTLADLKGKRISVGAAKSGTELNAR
                     TILGAAGLTYKDFRQVQYLPFGESVELMKNHQLDVTLQSAGLGVSSIRDLANSNAIVI
                     VPIPVDIVKKINDPAYQAAKIPANTYDGQTQDTPTVAVQNFLVTRKAVSDDEAYAMTK
                     QLFTHLNELVAAHNAATGITLDHAIVSLPVPLHPGAARYYKEVGLLKQ"
     gene            8042..>8238
                     /locus_tag="VFR20_04780"
     CDS             8042..>8238
                     /locus_tag="VFR20_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEU0229864.1"
                     /translation="MDHNKDGNDNLESGIDRRSLLKMGVGAAGGALLPGLPAVALAAD
                     SKPAIGNYPAGVSGDSVFIGLT"
BASE COUNT         1635 a         2398 c         2729 g         1476 t
ORIGIN      
        1 ggcaagccgc aggccggatg atggatcgtg tcgcgtcgcg taaagcgtgc cgagcaccgc
       61 cacacccagg gcggcgccgg cgatccgcgc cacgttgatc agggcggacg cggtgccgga
      121 acgcgcggcc ggtgcggaac ctacggccag gcccatcagt gggccggttg cgaaacccat
      181 gcccacgcct gtcagggtca ggccggcctc ggataacgca atggctgggt gtccggcgcc
      241 ccagccgatg gtcagcaagc caatgccgat gatcaaaacg cccccgcatg cgggctgatg
      301 cgcgccgaag gtctgtgaca accgccccgt cagtggtgaa atcaccagaa acaccagcgc
      361 catgggcatc agggccagcc cggcctgggt ggccgtcatc gcgcccattt ccagccatac
      421 cagcggttgc aggaacagga cgccgtacat accggacgtc atgccgactg tggcaatgat
      481 ggcgccccgc atgtccggaa tgcggaacaa ttccagcgga accatggcgg cagcgccttt
      541 gcgggcctcc gcgcggctga acaacgccag agccgccgca ccgatgatca acgccgccag
      601 cgacaacaca ccgtgcccgc gggcctcgat ggcggccagc gtcagtccgc cgagcgtcag
      661 ggcgcccagc acctgtccgg tcagatcagc gtggcggtcg gcgggatccg cggattcggt
      721 gatggatcgc caggccagcg ccagcgccag cccgcatacg ggaatggcca cgaagaacag
      781 gctgcgccat ccgaaccagg aaatcagcaa tccgcccagc gtgggggcga tggcgaatgc
      841 caggccgttg caggccgccc agattcccag ggcccggccg cgacggacag ggtccggcca
      901 cccgacgcgc agaatggcga gtgtcgcggg cgtgagcagc gcggcgccca cgccggcggc
      961 gacccgcccc gcgatcagga cgccgacgct cgacgccatg ccacacacga tcgacgcgcc
     1021 ggcgaacagt agaacgccag tcaggtaggc gcgtttgcgc ccccacaggt cggcgatcag
     1081 cccgccgctg agcaacagcg cggcatacac caggttgtag ctgtcgacca cccactgcat
     1141 ggacgtgata ccagcctgga aatacgcctt gatgggttgt acacccaggt tcaccacggc
     1201 ggtgtcgatc taggcgatga ggacggccag gcaaagcgcg gtcagcgcgg tcgacgtgcc
     1261 ggccgtcgcg ccactcaacg cctcccggct cgacgaagag gtgcccgggt catggggttc
     1321 agtgtcgggt aaaagagatg tgatggagga ttgtgtcatg atgtggatgc ggacaatgcg
     1381 cttgtggcga ttcttggatg gtggccgctt gtggcgggtg cattgtgttt attgccagta
     1441 ttgttttgtg gtggctggcg gcattcagac gccgaaacgt ttgccgcgat gggggggctg
     1501 cggcgctccc agtgcctgga cacacacatg ataggggcgg ggtgggtggc gatgcttcgc
     1561 tgcggaacga agcgtgacgc ggcatgcttc gggctggaat gcttcgttca tcatcgaagc
     1621 atttcccgcc gtcgaggtgt acccttgaat ggatcaaaca gagtgcgtcg cggttctctt
     1681 ctttaccaag agacagcgcg gggcctgcct accgcacatc atcgcgggga gttcagcgcc
     1741 gccaaggttg gtcggaacgt gtcgacgcaa aaggtattca ccgtggaaac tttcgctgat
     1801 ttttcttctg tcgcctatct gctcgccgac ccgtcccgag ccatcatgct cgcggcgttg
     1861 cttgacgggc gggccctgcc ggctggcgag ctggcccacg ccgccggcat caccgcgcag
     1921 acagccagtg cccatttatc caagcttttg gaaggcggtc ttctggcttg cgagagcgaa
     1981 ggccggcatc gttattaccg gttggccggg cccgatgtgg cccaggcgct cgagcatctt
     2041 gccgttctca gtggttccgt gcgccgcaag ccgctcggga ccgaaggcac tacattgcgc
     2101 caggcgcgat gctgctacga tcatctggcg gggcggttgg gggtggtcat cacgcaaagt
     2161 ctcgtcggcc acgcatacat tgtccccatt gacggcaagc agtacgagat cacctccgac
     2221 ggtatggaat ggttcgaaag cctgggcatc aaagggttgc agccgtcgcg gcgcgggctg
     2281 gcgcggcagt gcctggactg gacggaacgg gcgcaccatg tggcgggccc gcttggctcg
     2341 gggcttttac gcgcgttatg cgacctgggt tggttacgcc ggtcgcggtc gtcccggctg
     2401 gtgcaggtca ctcccgaggg gcatgccggc ctgaaggctc agcttggcgt ggacgtttca
     2461 tagagcgcgg tttggctagt tcccgtagtc aatccgcctg cacgagccaa accgcacacc
     2521 agccaccatg atggttctat tcaggattca cgatgcttac tgacggggcg cccgactggg
     2581 ctgatgcgcc ggaaggctgg gactggttgg cccaggatga agatggccgc tggttctggt
     2641 atggcgtgcg gccgcagccc agcctgggtg gcggtgtgtg gcgcgcgcca tcgaaggcgc
     2701 agcggttggc gctgcacggg acgccaaatc ctgattgggt ggattctttg catcaccgac
     2761 cagctttttg aatgtcgaac caacccagcc aaggattggc gatggcattt ctgacttggg
     2821 gtggggccaa catggcgtgc cgcaatccaa ccgtccacca cgaggaagta ttcacgccag
     2881 tcactccgct ccaacgtatc cctcgatgca ttccaatcag aaaatacgct accgcttggt
     2941 gtgatgctga atggttttcc cgtcgtagac gtagacatcg acctcatcgg gtttctgggc
     3001 gttgggcgtt tccatggtca acttgcggcc cgtggcgaaa accttgatct gctttgaaca
     3061 cgagccaaac gacggcgaaa cggtgtagcc tttttcgtcg accgtgatcc agcggtaagt
     3121 gaatgggcac tgatagctac ccttgttcac cgacaccagc gccacctgcg acgttggtgt
     3181 ggcgaaagcg taggtgatat tgacgatgcc ttcgatcctg ggttgcactg gtttgtcgtc
     3241 gaactgcagg aacgactcat acacgtaacc gctctctttg acgctcagcg cgccataagg
     3301 ggtgtcaagc gacagacgcg agggcgggac gggccagatc atttccgcgc actgggcggc
     3361 gctggcggcc accagagagc agagtgccag agcggtgctg agcaaggtcg ggataagtgg
     3421 gcgcatgttg cctgattgga acctggaaat ggcaaaaagt tgccgtggcg taacagtggc
     3481 cttgtcggga gacgaatcag gacaatgtgc gagtacgccg gcaccgacga attattgacc
     3541 cggcgcaagg gaaaagcaaa cgggcgaaag tactggggtg gcgtcggtca gaagccggtg
     3601 atggccatgt agatcagcat gagcagcatg acgcagaaat aggcgatgac acccgccacc
     3661 aggctcttgc cgatgtttct caggcgcgcc tgaagcgtga gggctttcgt ggcgaagggg
     3721 ccgcgcgaca gccaccacca gacgaaggcc atggcaagca gggtgaggag gatggcgccg
     3781 cgtttcatga tgcgtggggt ggatgtttga tccggggtgt atagacccta ccgcgagcag
     3841 ggttcgatga tcagagaacc gccggccgag atcggcacca gatcggccat ggggcggcag
     3901 gccccgttca cacagaaacg atagtcttgc gtgaaccggc tgcgggccag gttcaatcga
     3961 ggcagcggcg ggatgttggg atgatacgtc caataggcat gtttcgggtc aaaggtcgag
     4021 tcgggcggcg gttccatgcc cgccccgtcg ccttgcacat gcgcggcctt gagcaacagt
     4081 gtattgccct gtacggcgta atcctcactc cagtcgatct tctcgatgga gtggcgccac
     4141 acaagcgtga agtgatcggc cggcaccttg acggacaagg cgccggccat gaggcagatg
     4201 gcggccaggc tcatggcctg gccgtcggaa gggccgggga cgtgcgtgtg cgcatcaggc
     4261 gggctggcgc tgcaaacgct tgtgctggcg cgcggcgggc cagtgccaca gcagtagcag
     4321 aatggccaga ccgaatccga cctgatcggt ccacaccgac gccaccacca gcgacaacga
     4381 cgccacggcc gacagcgcgc gttgcggcca cgacagcggc gccagcagga acccgacgct
     4441 ggcaccgccc cacagggtga tggcgaccag ggccttgaac accatgtacg cggtgtcgta
     4501 ccaggcgccg ccctgcatca tcagcgacgg gtcatagaca gccatgtacg gaatgacaaa
     4561 accggcgatg gcgatctgcg tggccctgat ggcgatgcgc atgaacgatg ccttggcaat
     4621 ggacgacgcg gcgagcgcgg ccagcgcgac cggcggggtg aggtcggcca tgatcccgaa
     4681 atagaacacg aacatgtgtg acacgatgag cggcacgccc agcttgagca gggcgggcgc
     4741 ggcgatcgag ctggtgatga tgtagttcgg gatcgtgggg atgcccatgc ccaggatgag
     4801 gcagaccacc atggtcagga gcagcgacag aaacagactc ttctggccga cttcgaggat
     4861 gaagccggcg aagctggtgg ccgcgccggt gagcgtcagg ataccgatga tgatgccgac
     4921 gatggcgcac gccaacccca cgggcagcgc ctgcttggcg ccattgatga gcccgtctcg
     4981 gatcagcgtc agggtttgtt ttccgccctt gacgaatagg caacttatcg ccagcgcggc
     5041 gatgacgccg atgatgaccc agatcccgtg cttgaagaaa tcagccgcgg cgaggcctat
     5101 cacgatccag aagaagaacc ggaacgccat ggggctgatg gaggcggtga tgttcgctcc
     5161 gaggatcaac gtggccgtga gacccagacc ggtcagccct gcgaacatcg gtgtgaagcc
     5221 gtcgaacagc atgaacacca gcacccccag cggcagcagt aggtaccagt attccttcat
     5281 ggacttccac ggattcgggc agtcttcctt gggcaggccc aacagcccgg cgcgtccggc
     5341 ttccaggtgc accatccaga acgcggtcac gtagtacaac accgccggaa tgactgcggc
     5401 cttgcagatc tccaggtacg gaacgttcag cgtctcggcc atgatgaagg ccacggcgcc
     5461 catgacgggg ggcatgatct gcccgcccat gctggcggtg gcctcgaccg cgcccgcgaa
     5521 caccgagctg taaccgaagc gtttcatcag cggaatggtg aactggccga ccgtgagcac
     5581 gttggccacg cccgacccgc tgatggtgcc catgaagccg gacgaaatca ccgccacttt
     5641 ggccgggcca ccgcgcgaat gtccgacgaa tcccagcgcc aggttgttga acagccggat
     5701 catgccggcc tgctcgagga aggatccgaa caggatgaac aggaagatgt aggtggccga
     5761 caccagggtg ggcaggccat agatgccttc cacccccagg tacatctgcg agatgatctg
     5821 gtcaaaatag taggggcggt gcgccaacgc gccgggcagg tattcgccga acatgccata
     5881 cagcaggaag gcgccgcaga tcagcggcag cgccagaccc agtatgcggc ggcagctctc
     5941 gaacaacagg agcagtacca ccgagccgac caccaggtcg accaccgtgg gtgagcccgc
     6001 gcgcaggatg agatcggttt cgaagtacca gtgatagaaa ccgagggcga agcccgtcag
     6061 tgcgaggccc cagtcgtacc aggggatttt cttgccgacg gccgccttgg tcatggggaa
     6121 gatcatgaaa gccagcatca tcaggaaacc gacgtgcagg gaacgtatga cctggcttga
     6181 caacggactg aacgccgcga cgatgagttg atagaacgag aacaccaggg cgaaggcggc
     6241 gaagacgagg gccgccttgc cggtcaggga tcgttgatgg ccgagctcgt cgacctcgac
     6301 ctcgggaacg gggttgccga gattcgcgtc atgcatggaa gcatgggaaa ccatagagtg
     6361 cctttcgatt ttgcgggtgc cccgttgtgc gcggcaaccg aaacaggagc cgccgaggtt
     6421 gccggcggct ccgtggttcg ctgtgtgact ggatcactgc ttgagcaggc ccacttcctt
     6481 gtaatagcgg gcggcgcccg gatgcagcgg cacgggaagg ctgacgatag cgtgatcgag
     6541 tgtgatgccc gtggcggcgt tgtgcgcggc gaccagctcg ttcaggtgtg tgaacaactg
     6601 cttggtcatc gcgtaggctt cgtcgtcgga cacggctttg cgcgtgacca ggaagttttg
     6661 cacagcaacg gtgggggtgt cctgggtctg gccatcgtac gtgttggccg ggatcttcgc
     6721 cgcctggtag gccgggtcgt tgatcttctt cacgatatcg accggaatgg gcacgatcac
     6781 gatggcgttg gaattggcca ggtcgcggat ggatgacacc cccaggcccg ccgattgcag
     6841 cgtgacgtcg agctggtggt tcttcatgag ctcgaccgac tcgccgaagg gcaggtattg
     6901 cacctggcgg aagtccttgt aggtgagacc ggcggcgccc agaatggtgc gggcgttcag
     6961 ttcggtgccg gatttggcgg cacccactga gatgcgtttg cctttcaggt cggccagggt
     7021 cttgatgccg ctatcggcgc gggccacgat ctggatgtag ttcgaataga tgccggcgat
     7081 ggcgcgcagg ttgtcgagtt tcttggtgaa accgacttcg gcattgccct tccatgcatc
     7141 ggacagggaa tccgccaggg tgaaggccag ttcggcacgg ccggcctgca gcaggttcag
     7201 gttttccacc gatgccttgg tggactgcac cgacactttc acgtcgggca tggccttgct
     7261 gtacacctgc gacagcgcca cccccatggg gtagtagacg ccactggtgc cgccggtggc
     7321 gatgttcaga taattggcgg cgtgggcgac cggcgcggcc gcgaccaggg ttgcggcaca
     7381 ggcgccaagc aggaccttgt tcagggaaac agataaagaa aatttcatgg gctctcctcg
     7441 tggtttcatg aaatttccgg atgcgggcac ccggttggtt gaggcggcac cgcgttgccg
     7501 gcccatgaca gaacaccacc ccgaaaccgg ggtggcgaaa gaaaccatgg gcggaaatgc
     7561 gcggtactgt gccgccagag cgcggatcga ctcggctgcc cccctcagag cagcgtgtcc
     7621 gcgatatacg aaaggcggat tttagcctga ctgtgtcgga atcatgcgcg tttcatggcc
     7681 cgcccgaaca attcgaggga aaaccccgat tctgatcggt atgcggcgca tcagtcccgc
     7741 ctgttcgcct cacccgttcg agcgtcgatt cttcttgttt ggttgactca actttggtta
     7801 aaccttgatc aatgaaggtt ttccctatga atgaatgtct ttgaaaattt tatgctgtgc
     7861 gataaattaa aaaaaggatg ttcgtaacca tatatgaatc ccatggtgcg acaaatgccc
     7921 ggcaagcgta tcgctaccgg gcatatgtgt gttttagcgc gtcataaatg cgctttccga
     7981 aaccggcggc aagctcgttc tcgcagtcaa atccggttgg tttaaacaca ggagacgtta
     8041 gatggatcac aacaaagacg gcaatgacaa tcttgaatcc ggcatagatc gccggtcatt
     8101 gctcaaaatg ggggtggggg cagcaggggg ggcgttattg cccggcctac cggcggtggc
     8161 gctggccgcc gacagcaaac cggccatcgg caattatccc gcgggtgtca gtggcgattc
     8221 cgtgttcatc ggcctgac
//
LOCUS       DASTNK010000061        33013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_598960, whole genome shotgun sequence.
ACCESSION   DASTNK010000061 DASTNK010000000
VERSION     DASTNK010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 33013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33013
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598960"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VFR20_04785"
     CDS             complement(<1..359)
                     /locus_tag="VFR20_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229865.1"
                     /translation="MKHSLQVLSTALVLLGLQGLVPACAAEVRQVPAPVEEIHLQVGQ
                     LPSVKLTADILYRILAAEIAAQHGDFIAASQTMLDLSKDTLDPRLAKRAFQMASAGRD
                     MSEALDAAREWVLLAPD"
     gene            474..1301
                     /gene="mutM"
                     /locus_tag="VFR20_04790"
     CDS             474..1301
                     /gene="mutM"
                     /locus_tag="VFR20_04790"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEU0229866.1"
                     /translation="MPELPEVETTRRGIATIMPGRTLTRLVIHEPRLRWPVPDDIAER
                     VRDHPVLECGRRGKYLLIRFDNGTMIVHLGMSGSLRRVPLDASRRKHDHVEWLFADAR
                     FLLHDPRRFGAVLWHDAADGPITEHPLLTKLGVEPFDPAFTAAHLHAGLRGKASSIKS
                     VLLNGQVVVGVGNIYASEALFRARINPKAKAGSLSPKRCARLADAVRQTLDDALASGG
                     STLRDYVDASGEPGAYFALHAAVYDREGQPCRICQTPIRRIVQGQRATYYCPTCQRR"
     gene            1396..2352
                     /locus_tag="VFR20_04795"
     CDS             1396..2352
                     /locus_tag="VFR20_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0229867.1"
                     /translation="MTKQFASHADLEDKVVSFDKLSDNAYAYTAEGDPNTGIIIGDDA
                     VMVIDTQATPVMAQDVIRRIREVTDKPIKYILLSHYHAVRVLGASAYGAQEIIASRDT
                     YDLIVERGEQDKASEIGRFPRLFRAVESVPDGLTWPTITFEGEMTVNLGNLEVQILQV
                     GRGHTKGDTIAWLPEQKILFAGDLVEYQSTPYAGDCYFREWPYTLDTLASFSAEKLVP
                     GRGPSLRSAAEVRKGLAGTRAFLTDLYSCVNSGVADGKDLKTIYRETYAFMQPRYADW
                     VIFDHCMPFDVSRAYDEATGYDDPRIWTAERDLEMWKQLEGN"
     gene            2394..4100
                     /locus_tag="VFR20_04800"
     CDS             2394..4100
                     /locus_tag="VFR20_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0229868.1"
                     /translation="MGDIDYQTMEFTYRPQAPGPGRHPVVVVGAGPVGLTVALDLARR
                     GNRVLVIDDDFRLSTGSRAICFAKRTLDVWDRLGVGQRMVDKGVSWNVGKVFFKDKEV
                     WRFDLLPESGHRRPAFINLQQYYCEGYLYEQAELHPLIELRWKHKAVAANALPGGVEL
                     TIETPDGPYALQADWLIACDGSRSPTRRMLGEEAHGRMFRDRFLIADIKMRADFPAER
                     WFWFDPPFHPNQSVLLHSQPDNVWRVDFQLGWDADPVEEVKPENVIPRLKALLGDDVA
                     FELEWVSIYTFSCERMDHFRHGRVLFAGDSAHRVSPFGARGANSGVQDADNLGWKLDL
                     VLRGQAPETLLDSYASERGQAADENVRHSTRSTDFITPKSPISRLFRDTVLRLSRDHE
                     FARAFVNSGRLSTATRYTASPLNTPDTEQFAPSLAVGDTAPDAPITLAGQAGWWLSRL
                     GGPFVLAVFHPHRAPSPQVMETLGALCRHHANIQVALVRAVGGADDDAVIAGHFPPGN
                     LVVVEDTDGLLQKRYDARDGTCYLIRPDQHIAARWRGIDTDRIQAALRRAIGHSQQAN
                     PA"
     gene            4097..4303
                     /locus_tag="VFR20_04805"
     CDS             4097..4303
                     /locus_tag="VFR20_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2783 domain-containing protein"
                     /protein_id="HEU0229869.1"
                     /translation="MSSLNTRRNLPAPDDFYENLIDAHRDLSDTQSQALNAALVLLLS
                     NHIGDTDVLHEALAAARKIAREST"
     gene            4347..5651
                     /gene="hmgA"
                     /locus_tag="VFR20_04810"
     CDS             4347..5651
                     /gene="hmgA"
                     /locus_tag="VFR20_04810"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEU0229870.1"
                     /translation="MSTPTLLPGFGNHFATEALPGALPQGRNSPQRCPYGLYAEQLSG
                     TAFTVGRASNRHSWLYRIRPAAAEQQPFARYPGASAWSNQFNQGPVTPDPLRWDPLPA
                     PTTPMDFIDGMHTWAGNGHCDEQMGCGINLYAANHSMQGRFFYNADAEMLIVPREGRL
                     RLATELGRIEVEPCEIAVIPRGVRLRVELPDGAASGYMLENFGLPFRLPELGPLGSNG
                     LANARDFLTPRAWFEDEEGDFELIAKFTGGLWHAAIQHSPLDVVAWHGTHAPYKYDLR
                     HFNTVGSISYDHPDPCIFTVLTSPSHAAGIANVDFVVFPPRILVAEDTFRPPWFHRNA
                     ASEFMGLIQGRYDAKAEGFVPGGASLHNCMTPHGPDAATFEKAARADTHQPDHIRDTM
                     AFMFETGRVIRPTRAALESTTRQKRYTECWHGLARHFDPTHP"
     gene            5668..6996
                     /gene="fahA"
                     /locus_tag="VFR20_04815"
     CDS             5668..6996
                     /gene="fahA"
                     /locus_tag="VFR20_04815"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HEU0229871.1"
                     /translation="MTHSDLNETHDPALRSWVASANTADNGFPIQNLPYGIARRRGGD
                     EPLRPVVAIGAQMLDLAGLARAKPFQGVAADALDTCRGSTLNPLMALTPAHWSALRLA
                     LSRALREGSTLRDTLRPLLMNQADVEFGLPAHIGDYTDFYISIHHATAVGRLFRPDNP
                     LLPNYKWVPIGYHGRASSIGVSGQVFPRPVGQTRPAGDGQAPGFGPCQRLDYELELGV
                     LTGQGNAPGQRIPINQAENHAFGLCLLNDWSARDIQAWEYQPLGPFLAKNFATTISPW
                     VVTLEALAPFRAPFTHPASDPQPLPYLASAENSAGGAFDIDLDVLLSTRRSRAAHTEP
                     AVLSRSNFKYAYWTIAQMITHHTVSGCDLRPGDLLGTGTQSGPQAGQEGSLLELNQGG
                     KKPIALPWGETRKFLEDHDEVILRAACRKNGYPTIGFGECAGTVLPSLPT"
     gene            complement(7114..8526)
                     /locus_tag="VFR20_04820"
     CDS             complement(7114..8526)
                     /locus_tag="VFR20_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU0229872.1"
                     /translation="MAQTDELIQIVGKANVLTGGDAEPYCTDWRDRYHGRVLAVVRPG
                     STGEVADVVKWCAAHRVPIVPQGGNTGLCGGATPDGSGTALVVSLTRMNRIRAIDTDN
                     DTMVVEAGCILQAAQQAARDADRLLPLSLAAEGSCTVGGNLGTNAGGTQVLRYGNTRE
                     LALGLEVVTPQGEIWHGLRGLRKDNTGYDLRDLFIGSEGTLGIITAVELKLYPLPVAQ
                     CTALLGLSSVEDAVKVLSVARKGFGASLTGFELIAGNCLKGVVECYPQQRIPFDGPSA
                     QLPWYALLELSDSESAQHARERFEAVVGGMLESGLVPDAVIAENVAQSKALWHLRESI
                     PLAEKAFGKSIKHDVSIPVSRMAEFVRVTNAALQAAFPGIEHVIFGHLGDGNLHYNVA
                     RGQAFTEQELLDRQYAVYELVHDSVHRFAGSISAEHGVGQLKRDALPKYKDPVELAIM
                     RKIKQALDPDGLMNPGKVLA"
     gene            8680..10071
                     /locus_tag="VFR20_04825"
     CDS             8680..10071
                     /locus_tag="VFR20_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEU0229873.1"
                     /translation="MPATPPESVFKAYDIRGIVPSQIDAGFAARLGRVLGRMAREQGI
                     EALVVGRDGRLSSPSLASGLVDGLLAEGVDAIDIGLVPTPVVYFAAHVLETGSGVAIT
                     GSHNPADYNGFKLMLGGRTLHGDDITTIGRAMSATPADHRPPARAGTRRGLDILDAYV
                     SRICQDVKLARPMKIAIDCGNGAGGVVAARLFETLGCQVDRLFCDVDGRFPNHHPDPS
                     VPGNLRDLQAHLASSDCELGLAFDGDADRLGVVARDGEIIWPDRQLILFARDVLSRHP
                     GATIIYDVKCSRHVGREIEASGGHALMWRTGHSVMKAKLAETGSPLAGELSGHIFFKD
                     RWYGFDDGLYVGARLLEILSRTPDASTTLRGLPVGIATPELKIAVPEGEQHTLVARLR
                     TEAAFPSATGIVAIDGVRAEYPDGFGLARASNTTPAITLRFEGDTPQALTRIQDEFRL
                     ALSRLAPNATLPF"
     gene            complement(10159..11661)
                     /locus_tag="VFR20_04830"
     CDS             complement(10159..11661)
                     /locus_tag="VFR20_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006493060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU0229874.1"
                     /translation="MKQSTRTNDLMWSELFARFKTQTNTLQGQLRDTLMHAVIEGFIL
                     PGEALPSSRFLAQKLGLSRTTAMAALQALTESGIVVGHPRSGYIVAGEGQSIQPDVRK
                     PQTDSAGRERVAWGRRLMFTPSRQQNIEKPVDWQNQPYPFIYGQFDASLFPTLQWREC
                     VLETLQPRVLRHWAPDHLDRDDELLIEQIQRRLLPARGIWVEQDEILVTTGAQQATFL
                     LSELLIDSKTVVGMENPGYPDARNNFSLRSRDVRFLDVDGEGLVSGPALSDCDYVYVT
                     PSHQCPTTVTMSLERRHMLLAHAARDDFVIIEDDHESELNFSGRPSPALKSLDSAERV
                     IYVGSLSKTLAHGLRLGFVVAPADLIRELRALRRLMMRHVPTNNQRSAASFIAHGYHE
                     AFVRRLNIVYKQRATALRLALAEHIPGLMLVPAQGGSALWVTGPENLDARKLARNLYV
                     KGVVVEPGDVFFAGNEQPGNHMRIGYSSIEADRIEGGVRIIAQEIRREVA"
     gene            11784..13094
                     /gene="hisD"
                     /locus_tag="VFR20_04835"
     CDS             11784..13094
                     /gene="hisD"
                     /locus_tag="VFR20_04835"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEU0229875.1"
                     /translation="MRYIKSAANQKAEAENRTIRQQVETMLSDIEKRREQAVIDYARQ
                     LDGWEDKFVLSRSKLATLIQEVPDQLKQDIHFAHRQVTRFAQAQRDSLSGFEMETEPG
                     VRLGQRIVPVSAAGCYVPGGRYAHVASAIMSVATAKIAGVQGVVASSPPRGHSINPAT
                     AYAMEISGADIILEMGGVQAVATMAYGLFDCPQADIIVGPGNAYVAEAKRILFGKVGI
                     DVFAGPTESAVIADESADPMTIAIDLASQAEHGTNSPVWLFTTSESIGEAVSRLMPKV
                     AGDMPNASVVNTAWHDHGVIVLCESREEMAKVSDQYAPEHVQVLADDLPWWKENLRNY
                     GSLFLGEGSTVSHGDKCSGTNHILPTSGAGRYSGGLNVQKFLKVLTYQELSPEANKAF
                     SAVGSRLSRAEGMEGHARACDWRLHKYYPDTRWDFDVYKQERYV"
     gene            13110..14303
                     /locus_tag="VFR20_04840"
     CDS             13110..14303
                     /locus_tag="VFR20_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU0229876.1"
                     /translation="MEKFRKSFTQQEPIPESGISRAVEVMRSGRLHRYNVNEGEKSEA
                     TILEEKFAHYLGVRFCLACASGGYALTTALRAFGIKANEPVLTNALTLSPVPGAIHSA
                     GAHAVLVDTTEQLVLDLDDLDRKLASTSARLVLVSHMRGHIVDMDALCAILARHGAAL
                     IEDCAHTMGASWNGRPSGTFGIAACFSSQTYKHINSGEGGFLTSNDPGLMARAIILSG
                     SYMFYDHHTAGPAPEAYQETRMVTPNCSGRMDNLRASILIPQLEALDDRVEQWNIRYR
                     ALEAGLIDVPGLKLPCRSPKEHYVGSSIQFLIPGITAQKAEELLAICQRQGVVLKWFG
                     APEPRGYTSHYASWRYLTPQTLPRTDQVLNSLFDMRIPLTFSVEDCHLLGNIIRNVVK
                     ALHHS"
     gene            14363..15307
                     /gene="tauA"
                     /locus_tag="VFR20_04845"
     CDS             14363..15307
                     /gene="tauA"
                     /locus_tag="VFR20_04845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_function: GO:0030977 - taurine binding [Evidence IEA];
                     GO_process: GO:0015734 - taurine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0229877.1"
                     /translation="MTVGISTVAHAEKLIIGTFGDPTPAQAAIAQGKFAKATGWNIEW
                     RKFESGASVISAMASGDVQISELGSSPLAIATSQGLDLKLFLVSFLINTSESLIVRNG
                     TGIDKPADLKGKVIGVPIGSTSQLSLTGALKHWGISENQVRILGMTPQQINAAWARKD
                     IDAAFVWNPVQSELLKNGKRLVSAGQVAKWGYPTFNGWVASSTFVAKHPKGVAAFAKA
                     MNEANAAYLDNKSKWTADSETVKQIAKRTGAQPAQVPSILQGFTFLTASEQTAWLSTH
                     AADALKKTAIFLKKNHRIDTVKNNYGEFVDTAVAKSVQ"
     gene            complement(15366..16535)
                     /locus_tag="VFR20_04850"
     CDS             complement(15366..16535)
                     /locus_tag="VFR20_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0229878.1"
                     /translation="MRRSDAQRTQHPDTRHLLFVVNNPAFFLSHRLPLALAAKRAGFE
                     VHVATMDGPSVSRIVSHGLAHHVIPMSRSGKNPLRELHSLFALWRLFRRLRPDLVHAV
                     TIKPVLYGGIAARLARVPAFVAAVSGLGYVFMRGDGRFDPLRSAAVFLYKLALGHRNS
                     RVVFQNTADRDVLRHAGVVREDQSVLIRGSGVDLDVFAASDEPDSPVRAVMASRLLRD
                     KGVEEFVEAARLSAARRTGVCWVLAGSVDPGNPASVSADTLAAWLREDVVDYLGEQDD
                     VAALYRGAHIAVLPSYREGLPKSLIEAAACARPVVTTDVPGCRDAIDADVTGLLVPAR
                     DAGKLAEAVARLAADPALRRKMGAAGRDLAEREFDVRKVEQAHLAIYDALIACSD"
     gene            complement(16635..17720)
                     /locus_tag="VFR20_04855"
                     /pseudo
     CDS             complement(16635..17720)
                     /locus_tag="VFR20_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(17717..18847)
                     /locus_tag="VFR20_04860"
     CDS             complement(17717..18847)
                     /locus_tag="VFR20_04860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0229879.1"
                     /translation="MKIMLLVSSMHAGGAERVAATLASAWAVRGDSVTLVPTFTGRGT
                     CFYPLASEVELCWLADIGPAGGAAPSGLRKLRVLRRLVRQRRPDVIVSFLTNVNVMAL
                     LSTIGLRVPIIVCERTNPAASDNVGRLLGWLRRVTYPWANMVTVQAQDSVQALREKVA
                     RMRALGVVANPLPPELPDMALAARVRDVRGRRRLAAMGRFVPAKRFDWLIRAFAQLAG
                     EFPDWDLVIWGDGPMREALTNQIRDVGLSDRVFLPGRTPEPWRELSRVHAFALVSSVE
                     GFPNALLEAMALGLPCIAADCPSGPREISQDGEDALLFPVSDPDALVAGLRQLLGDES
                     LRDRLARGAALSVRQRYGLPAVLAAWDDLMARAGVAVPGAGS"
     gene            complement(18844..20763)
                     /gene="asnB"
                     /locus_tag="VFR20_04865"
     CDS             complement(18844..20763)
                     /gene="asnB"
                     /locus_tag="VFR20_04865"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEU0229880.1"
                     /translation="MCGIVGVWGELPDRRAVIERGCAAIFHRGPDSRGYWEDREAELG
                     LGHVRLAIQDLSDAGHQPMVSASGRFVLVLNGEIYNHLDLRDRLRAEGSAPAWRGHSD
                     TETLLACLAAWGIAATLERAVGMFALGLWDRYDRTLSLARDRMGEKPLYLGYAGSRLV
                     FASELKALVDVPGFDPTLDPSALSLLLRHNYIPAPWCIYKALRKLRPGTFMTVSEAQL
                     RQRGLPDPVQYWSAQAVARDAIEHRLAFNSDDQAVDALEQVLSESVKGQMLSDVPLGA
                     FLSGGIDSSVIVALMQAQGGNAVRSFSIGFDDPAYDEAGYAREVARHLGTRHTELYVS
                     ARDALDVVPRLANLYDEPFADSSQIPTVLVTRMAREHVAVALSGDGGDELFGGYSRYF
                     RAQRWWQRRSALPGLVRRPAGVLGHTAAAMMPGGRVRERVERLSNALSARHAGHFYQS
                     FVSYWKDPAQALVAAGHPDSLFDQPSPDSFFDHMTLLDTVTYLPGDILVKVDRAAMAV
                     GLETRVPMIDHRVFEFARRLPHEYKVRDGHGKDLLRRLLCRHVPEAMVNRPKKGFSVP
                     LAHWLRGPLRDWGAALLDPVRLRDDGVFQARPVLRKWQEHQSGARDWSTHLWSVLMMQ
                     AWLDESRRAVRGEAT"
     gene            complement(20771..21937)
                     /locus_tag="VFR20_04870"
     CDS             complement(20771..21937)
                     /locus_tag="VFR20_04870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0229881.1"
                     /translation="MVSKASSVSRHLMIVIHSLQGGGAERVAADLGAHWVSRGYRVTV
                     VTQTGAEGDAYALHPDVRRVVLGTAGGSPGRIRGLLANLRRVFRLRRLIRRERPDIVL
                     GMMTTSSVLAVIAARRLPCRVIATEHTHPPSQSLPPMWLRLRCWAYPRAAAVVALTKG
                     TAAWIERNVPGSRLSVIPNAVRWPLDPAEPMLAVPDRAGRHRLLAVGRLHPQKGFDLL
                     IRAFASIAALFPDWDLVILGEGTQRDVLQSQVHASGLSDRVSLPGRAGNVGQWYESSD
                     LYVLSSRVEGLSNTLLESMASGLAAVAFDCETGPREVVRDGIDGVLVRPAEDWEALAA
                     HLSNLMAHPDLRAAYARRATDVRDRFSTARVMAMWHHVFEGDRDHRSTVAGGGE"
     gene            complement(21970..23346)
                     /locus_tag="VFR20_04875"
     CDS             complement(21970..23346)
                     /locus_tag="VFR20_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEU0229882.1"
                     /translation="MKTLARRLSTLHSDHRRIARGAARVAFFLLLGKFAGAAKEMAVA
                     YRYGVSNATDAYQFTMTMANWLPVTIVGVLAVVLIPVLVRVRYGDKADWSRFVGELQG
                     WVMVLGVVLAVLIYALWPYVLDVAGHGLTDEARRMSRELTLAFAPAAFLTLMTGISGA
                     RLRAHERHINTLLDSVPALSILVLVLLAGSVRDVGPLLWGTLVGYLVQSVWLFWLASR
                     ADGFRGRPRLTCRSERWPDLTRAAGVMLIGQVAMSFVGPIDQYTAATLGSNANATLGY
                     AARLLSLVLGIGAASVGRAALPVLADIQGRGDAHHARAMALKWSALMLLAGALAALVG
                     WLLAPWGVALLFQRGAFTAQNTQVVAHVLRWGLLQLPFYFGVLILVQLLASQNRYAIM
                     AAIAVANFVLKAIMNYVLAPSMGAAGIMLATSVMYALSYSCYVVVVLRLPAVSGGKAA
                     SGSTHRPH"
     gene            complement(23348..24373)
                     /locus_tag="VFR20_04880"
     CDS             complement(23348..24373)
                     /locus_tag="VFR20_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0229883.1"
                     /translation="MSTRYQEVVAGLQAAPRTWLVTGCAGFIGSNLLETLLLMGQKVV
                     GLDNFSTGYQHNLDEVRKTVSAAQWGNFTFHKGDIRDLDACRAAMPGVDYVLHQAALG
                     SVPRSLADPITTNDVNIAGFLNMLVAARDAKVKSFVYAASSSTYGDHPGLPKVEDQIG
                     RPLSPYAITKYVNELYADIFARSYNFGSIGLRYFNVFGKRQDPDGAYAAVIPKWVAAM
                     IRGEDVVVNGDGETSRDFCYVENAVQANILAALAQPDGVNQVYNVAFNARTSLNQLFE
                     YLAQALANNGIVYDKPPIHGDFRAGDVRHSQADISKAKRLLGYEPMHSIVQGLEVAMP
                     WYTQFLR"
     gene            complement(24462..25736)
                     /gene="tviB"
                     /locus_tag="VFR20_04885"
     CDS             complement(24462..25736)
                     /gene="tviB"
                     /locus_tag="VFR20_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HEU0229884.1"
                     /translation="MQIQDVKLAVVGLGYVGLPLAVEFGKKRSVLGFDINSNRVAELK
                     QGRDHTLEVEPAELAQAAQLSFSDDPSQLAQANVFIVTVPTPIDEYKQPDLTPLVRAS
                     ETIGKVLKRGDIVIYESTVYPGATEEVCVPVLERVSGLRFNDEFHAGYSPERINPGDK
                     SHRVSTIKKVTSGSTPEVADLVDALYKQIITAGTHKAPSIRVAEAAKVIENTQRDVNI
                     ALINELALIFNKMGIDTLAVLEAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAQAIG
                     YHPEVILAGRRLNDSMGGYVAAQLVKAMTKRSIQVRESRVLVMGLTFKENCPDLRNTR
                     VVDIVRELGEYNVRVDVYDPWIDADEAEHEYGIRPVAAPESHAYDGIVVAVAHHQFAA
                     MGAQAIHALGKPEHVLYDLKYVFDAADADLRL"
     gene            complement(25799..26938)
                     /locus_tag="VFR20_04890"
     CDS             complement(25799..26938)
                     /locus_tag="VFR20_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0229885.1"
                     /translation="MTPPQVWSSFSWLYICIVAFLVGGLIVVSERWHGRFTGDNDLHK
                     PQASHARAAPRVGGLAVVAGSLAGLLVLGPSNMTLTWLWPVLFIAAMPVFVAGLLEDI
                     TKDIGSGKRLLAAFVSAAIAWWLLGGVSRVGFSGVDWLLSFWPVSLIFTMVAVGGCTH
                     ALNIIDGMNGLAGMIATLMSLSLALVALQVQDIPIFLIAAALASATLGFLVWNFPFGR
                     VFLGDGGAYFVGFILAELAVQLVVRNPSVSPFYALAVLFYPVFETLFSIWRRRFKRGV
                     PVDQPDALHLHQLVFRRLVRVTFSRDRRHALPALCNAMTSPYLWVLALIGLVPATIWW
                     DNTWMLCASLVVFSAAYIWLYVRLVTWRRPSWLLLPSLGRDNRHK"
     gene            complement(27559..28218)
                     /gene="coq7"
                     /locus_tag="VFR20_04895"
     CDS             complement(27559..28218)
                     /gene="coq7"
                     /locus_tag="VFR20_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HEU0229886.1"
                     /translation="MSNESIPFRRENLPDRLLAEADRALRVLGGVARAQRGNPAGRPE
                     AHESLSPSDQRHAAALMRVNHVGEVCAQALYRGQSLFCRDAEVRGVLARAASEEVDHL
                     AWCRDRLGELHSRPSYLNPLWYAGAFALGTLAGRAGVGRNLGFMAETEAQVEHHLDSH
                     LVRLPADDKRSRRIVEQMRDDEAGHGDTARRHGAGPLPAPVRLSMKCMAKIMTTTAYW
                     L"
     gene            complement(28384..29016)
                     /locus_tag="VFR20_04900"
     CDS             complement(28384..29016)
                     /locus_tag="VFR20_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229887.1"
                     /translation="MIAANHFGSLSSRRFVCIALSVMLLLVAIILRHPLTRTMALHML
                     VHIPLVVAAGALAARGLESHGRNRPTGPASKALAVYRRFDEYGVPGLLLASFAGAFWM
                     IPRALDEVLSSPYADAWKFISLFAIGLVLRDALARAKPVIRLFFLGNFCWMAAVVGIV
                     YQNIPSRLCNFYLASDQQVAGMGLVALSVLLPLAWLLAAWRRVRVFLRDT"
     gene            complement(29013..29768)
                     /locus_tag="VFR20_04905"
     CDS             complement(29013..29768)
                     /locus_tag="VFR20_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEU0229888.1"
                     /translation="MERESRAGQAQIARDRRSLRLVVALSLVCAIGLGAIGVATDGYA
                     VVTTEAARRLSIAAHPRSLPDVALANGSAVGGSLHGLLRQDGRVAIVDFIYTRCFSLC
                     LAMGSEFQQLQDTIERRGLADRVRLISISFDPADTPDDLARYARDLHADPTVWRFYGA
                     PTRAGLEILLRAFGVVVVPAPLGQFVHNAAYHVITPDGRLTRVVDYGASGEALRFAAG
                     EAAQRVLERAPVRTESKQGWGVPARPAQGGVKP"
     gene            complement(29732..30337)
                     /locus_tag="VFR20_04910"
     CDS             complement(29732..30337)
                     /locus_tag="VFR20_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU0229889.1"
                     /translation="MSDNNAPTESTRAQRREMPEPFESNNPVPWYVIVLLAGLFIWAV
                     GYIWLNYSDEPSAMGDHRVAADFAVAKAGAGGAVNGAQLYTSHCLACHQATGQGLPGV
                     FPPLAGSEWVDGKASTMIQIVLHGINGSLTVKGATYKGQMPTFKDQLSDAEIAAVISH
                     VRTSFGNSAGKVTVDEVKAERAKTKDHTAPWNGNPELDKLK"
     gene            complement(30393..30992)
                     /locus_tag="VFR20_04915"
     CDS             complement(30393..30992)
                     /locus_tag="VFR20_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HEU0229890.1"
                     /translation="MESEVKLLSGAMVTLALATATLVVVPYMRLSHVKPAEGLKPYTA
                     AQLRGRNVYIANGCVYCHSQQPRSQSQTPADFKRGWGRPGVAGDYYYDNPHLLGTMRT
                     GPDLFNIGARQPSADWNLGHLYEPRAYVPGSIMPSFPFLFEVKDKADKGDRVVTLPPN
                     YAPVGKVVVAKPEALDLVQYLLGLNHTYPIKNVPANLGK"
     gene            complement(30995..32653)
                     /locus_tag="VFR20_04920"
     CDS             complement(30995..32653)
                     /locus_tag="VFR20_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEU0229891.1"
                     /translation="MDTITILLLTFIISVTGLFIFIWSLRHQVFDPNPAGARVIFSEG
                     EIGHVEEPALTAAQRKSLQEAVGIPATDPARDERLRVELEERLQADKSTSFVAFVFLS
                     CAIVWLVFASFAGLISSIKLHSPEFLTQYAWLTFGRIRTLHLNAVAYGWAPMSAFGVV
                     MWALPRVLKTELVGGRFAVLGAMLWNAGLIAGLGSIAAGFNDGLEWLEIPWQVSILLV
                     VGGALIGLPLVYTLQNRKVDHLYVSVWYWGAALFWFPVLYLVAKIPGLHFGVEQATMN
                     WWFGHNVLGLYYTPIALGTIYYFLPKIIGRPIQSYNLSLIGFWALAFFYGQVGGHHLE
                     GGPVPGWLVTLSIVQSMMMLIPVIAFSVNQHLTMRGHFKTLIYSPTLRFIVLGGMMYT
                     LSSVEGSFEALRSINTVTHFTHFTVAHAHLGLYGFVTLVMFGAIYFVMPRVMSWEWPY
                     PKLVAAHFWLVVTGFSIYFIGLSIGGILQGLDMLDPSKPFMDSVRVTIPYLESRTVGG
                     SLMTLGHLVFAFHFVAMALRHGPSRIGPALFHKRKAPAAAMVEA"
BASE COUNT         6731 a        11095 c         9815 g         5372 t
ORIGIN      
        1 tcgtcggggg ccaggagcac ccattcacgc gccgcatcca gcgcttccga catgtcgcgc
       61 cccgccgacg ccatctggaa cgcccgcttg gccaggcgcg ggtcgagcgt gtccttggag
      121 agatcgagca tggtttggct ggcggcgatg aagtcaccgt gctgggctgc gatttcagcg
      181 gccagaatgc ggtagaggat atccgcggtc agcttgacgg agggcagctg cccaacctgc
      241 agatggatct cttcgacggg cgcggggacc tgccgcacct cggctgcaca ggccggaacg
      301 agcccctgca ggccaagcag caccagggcc gtcgacaaga cttggaggga gtgtttcatt
      361 cagttggacc actggcgccg ccgctacact tggcgcacaa aacggactat cgtacaagca
      421 gcacacaaga tggctgatca gccatccgga tttcccgatg ttagcacccc cgtatgcctg
      481 aactgccaga agtcgaaacc acgcgtcgcg gaattgcgac gattatgccg ggccgaacct
      541 tgacacgcct cgtgattcat gagccgcgcc tgcgctggcc cgtgccggac gacatcgctg
      601 aaagggtccg tgaccaccct gtgctggagt gcgggcgccg aggcaagtat ctgctcatcc
      661 gcttcgacaa cggcacgatg attgtccacc ttggcatgtc gggctcgcta cggcgtgttc
      721 cgctggacgc gtcgcgccgc aagcacgacc acgtggaatg gctgttcgcc gatgcgcgct
      781 tcctgctgca cgatccgcgc cgattcgggg ccgtgctgtg gcacgatgcg gcggatggcc
      841 cgatcaccga acaccccctg ctgacgaaac tgggcgtgga gcctttcgac ccggcattca
      901 ctgcggccca cttgcacgcc ggcttgcgcg gcaaggcgtc gtcgatcaag tccgtgctgc
      961 tgaacggcca agtggtggtg ggggtgggca acatctacgc gtcagaggcg ctgttccggg
     1021 cgcggatcaa ccccaaggcc aaggccgggt cgctgtcgcc gaaacgctgc gcccgcctgg
     1081 ccgacgcagt gcgccagaca ctggacgacg cactggcatc gggcggcagc acgctgcgtg
     1141 actatgtcga cgccagcggc gagcccggcg cctactttgc cctgcacgcc gccgtctacg
     1201 atcgtgaagg acagccctgc cggatctgcc agaccccaat acgccgcatc gtccagggcc
     1261 aacgggcgac ttactactgc cccacatgcc agcgacgata atcgtctggc ccgagcccaa
     1321 taattagtct atgacaaacg tattgcataa aatcaaacat tcaactatag tgcccccatc
     1381 acttcggagc cgcccatgac caagcaattc gcctcgcacg ccgacctgga agacaaggtc
     1441 gtctcgttcg acaaactgtc cgacaacgcc tacgcctaca ccgccgaggg cgatcccaac
     1501 accggtatca tcatcggcga tgacgccgtc atggtgatcg acacccaggc cacccccgtc
     1561 atggcgcaag acgtcatccg ccgcatccgc gaggtgaccg acaaacccat caaatacatc
     1621 ctgctgtcgc actaccacgc tgtgcgcgtg ctgggcgcct ccgcctatgg cgcccaggaa
     1681 atcatcgcca gccgcgacac ctacgacctc atcgtcgaac gcggcgaaca agacaaggcc
     1741 agcgagatcg gccgcttccc ccgcctgttc cgcgccgtgg aatccgtgcc agacggcctc
     1801 acctggccca ccatcacgtt cgaaggcgaa atgacggtca acctgggcaa cctggaagtg
     1861 caaatcctgc aggtcgggcg cgggcacacc aaaggcgaca ccatcgcctg gctgcctgaa
     1921 cagaaaatcc tgttcgcggg cgacctggtc gaataccagt ccacgcccta tgccggtgat
     1981 tgctatttcc gcgagtggcc gtacacactc gatacgctgg ccagtttcag cgcggaaaaa
     2041 ctggtgcccg gccgcggccc gtcactgcgg tcggccgccg aagtgcgaaa gggcctggcc
     2101 ggcacgcgcg cttttctcac cgacctgtac tcttgcgtga attcgggcgt ggccgacggc
     2161 aaggacttga agaccatcta ccgcgaaacc tacgccttca tgcagccacg ctatgccgac
     2221 tgggtcatct tcgaccactg catgccgttc gacgtctcgc gcgcctacga cgaggccacc
     2281 ggctacgacg accccagaat ctggaccgcc gagcgcgacc tggaaatgtg gaaacaactc
     2341 gaagggaact gaagtccgcc ccccactgac aaccactatc cgggtatcgg gccatgggag
     2401 acatcgatta ccagacgatg gagttcacct accggccgca agccccgggg ccgggacggc
     2461 atccggtggt cgtggtggga gcggggccgg tcggcctgac ggtcgcgctg gatttggcgc
     2521 ggcgcggcaa cagggtgctg gtcatcgacg atgatttccg actatccacc ggctcgcgcg
     2581 ccatctgctt cgccaagcgc acgctcgacg tctgggatcg cctcggggtc gggcagcgca
     2641 tggtcgacaa aggggtgtcc tggaacgtcg gtaaagtgtt cttcaaggac aaggaagtct
     2701 ggcgcttcga tctgctgccg gaatcgggcc accggcgtcc agcgttcatc aacctgcaac
     2761 agtattattg tgaaggctat ctatacgaac aagccgagct gcacccgctg atcgagttgc
     2821 gctggaagca caaggccgtg gccgccaacg cactgcccgg cggggtcgaa ctcacgatcg
     2881 aaacacccga tggcccatac gccctgcaag ccgattggct catcgcctgc gacggctcgc
     2941 gctcgccaac ccgcaggatg ctgggcgagg aagcccacgg ccgcatgttc cgcgaccgct
     3001 tcctcatcgc cgacatcaag atgcgcgccg acttccccgc cgaacgctgg ttctggttcg
     3061 acccgccatt ccaccccaac cagtcggtgc tgttgcacag ccaacccgat aacgtctggc
     3121 gcgtggattt ccagttgggc tgggatgccg atccggttga agaggtcaaa cccgaaaacg
     3181 tcatcccgcg actcaaggca ttgctgggcg acgatgtcgc cttcgaattg gaatgggtca
     3241 gcatctatac cttctcttgt gaacgcatgg accacttccg ccacggccgg gtgctgttcg
     3301 ccggagactc agcgcatcgt gtctcgccat tcggggcacg gggtgccaac agtggcgtac
     3361 aggatgccga caatctgggc tggaagctcg atctcgtctt gcgcggccag gcccctgaaa
     3421 ccctgctcga ctcctacgcc agcgaacgcg ggcaagccgc cgacgagaac gtgcgtcatt
     3481 cgacgcgatc cactgatttc atcaccccga agagccccat cagccggcta ttccgcgata
     3541 cggtgctgcg gctgtcgcgt gaccacgaat tcgcacgcgc ctttgtcaac agcggccggc
     3601 tgtccacggc aacgcgctat acggcgtccc cgctcaacac gcccgacacg gagcagttcg
     3661 cgccatcatt ggccgttggc gacaccgcgc cggatgcccc catcaccctc gccggccaag
     3721 ccggctggtg gttgagccgg cttggcgggc cgttcgtgct ggccgtgttc catccacatc
     3781 gggcgccatc gccccaagtc atggaaacac tcggcgcgct gtgccgtcat cacgccaaca
     3841 tccaggtagc gctcgtgcgc gcggtcggcg gggctgacga cgatgccgtc atcgcgggcc
     3901 atttcccacc tggcaacctg gtcgtcgtcg aagacaccga cgggctactc caaaaacggt
     3961 acgacgcgcg cgatggcacg tgttacttga tccggcccga ccagcatatc gccgcgcgct
     4021 ggcgcggcat cgacaccgac cgcatccagg ccgcgttgcg acgcgccatc ggccactcac
     4081 aacaggcgaa cccggcatga gctctctcaa cacccggcgc aatctgcccg ctcccgacga
     4141 cttctacgaa aacctgatcg atgcgcatcg ggacttgagt gacacccaaa gccaggcgtt
     4201 gaacgccgcg ctggtgctac tgctatcgaa tcacatcggc gacacagacg tactgcatga
     4261 agcgctggcc gccgctcgga agatcgcacg ggaaagcacc tgattcccgt gcatcagcgc
     4321 ccaaccatca caggagccgc cgtatcatga gcaccccgac gctgttaccg ggcttcggca
     4381 accatttcgc caccgaggcc ctgcccggcg ccctgccgca agggcgtaat tcgccgcaac
     4441 ggtgcccgta tggactgtat gcggaacagc tctcgggcac ggccttcacc gtgggccggg
     4501 ccagcaaccg tcattcatgg ctgtaccgca tccggcccgc cgccgccgag cagcagcctt
     4561 tcgcccgcta cccgggcgcc tccgcatggt cgaaccagtt caatcagggt ccggtcacac
     4621 ccgacccgct gcgctgggac ccgctgccgg cacccaccac cccgatggac ttcatcgacg
     4681 gcatgcacac ctgggcgggc aacgggcact gcgacgagca gatgggctgc ggcatcaatc
     4741 tgtatgccgc caatcactcc atgcaaggcc gcttcttcta caacgccgat gcggaaatgc
     4801 tcattgtgcc gcgcgaaggg cgcctgcgcc tggccaccga actggggcgc atcgaagtgg
     4861 agccgtgcga gatcgcggtc attccccggg gcgtgcgcct gcgtgtcgag ttgccggacg
     4921 gcgccgccag cggctacatg ctcgagaact tcggcctgcc attccgcctg cccgaattgg
     4981 gtccgctggg gtcgaacggc ctggccaacg cgcgggactt cctgacgccc cgcgcctggt
     5041 tcgaagacga ggaaggtgac ttcgaactca tcgcgaaatt caccggaggc ctctggcatg
     5101 ccgccatcca gcattcgccg ctggacgtgg tcgcctggca cgggacgcat gccccctaca
     5161 aatacgacct gcggcatttc aacacggtgg ggtccatcag ctacgaccat cccgacccct
     5221 gcatcttcac agtgctcacg tcgccgtcgc acgccgccgg catcgccaat gtcgactttg
     5281 tcgtttttcc gccgcgcatc ctggtggcgg aagacacatt ccgcccgccc tggttccatc
     5341 gcaacgcggc cagcgaattc atggggctga tccaaggccg atacgatgcc aaggccgagg
     5401 gtttcgtgcc aggcggcgcc agcctgcaca attgcatgac cccacatggc ccggacgccg
     5461 ccaccttcga gaaggcggcg cgcgccgaca cccaccaacc cgaccacatc cgcgacacca
     5521 tggccttcat gttcgaaacc ggacgcgtga tacgcccgac acgggccgcg ctggagtcca
     5581 caacgcgtca gaagcgttac acggagtgct ggcatggcct ggcccggcat ttcgacccca
     5641 cacacccctg atatcagaag gcgtgacatg acccattccg atctgaacga aacccacgat
     5701 ccggcgctac gcagttgggt ggccagcgcc aacacggccg acaacggctt ccccatccag
     5761 aatctgcctt atggcatcgc ccgtcgtcgt ggcggcgatg aaccgttgcg ccccgtggtg
     5821 gccatcggcg cgcaaatgct cgatctggcg ggtctggctc gggccaagcc gttccagggt
     5881 gtggccgcgg acgcgctgga cacgtgccgt ggcagcacat tgaaccccct gatggcgctg
     5941 acgcccgcgc attggtccgc tctgcgcctg gcgttatcgc gcgcgctgcg cgagggctcg
     6001 acactgcgcg acaccctccg cccgttgctg atgaaccagg ccgacgtgga attcgggcta
     6061 ccggcgcaca tcggcgacta caccgatttc tatatctcca tccaccatgc caccgccgtc
     6121 ggccggctgt tccgccccga caatcccctg ctgccgaact acaaatgggt gcccatcggg
     6181 tatcacggcc gcgcctccag cattggcgtg tcggggcagg tgttcccacg ccccgttggc
     6241 cagacccgcc cggcgggcga cggccaggcg ccgggttttg gcccctgcca gcggctggac
     6301 tacgaactgg agctgggggt gctcaccggg cagggcaacg cgcccggcca gcgcataccc
     6361 atcaaccagg cggaaaacca cgccttcgga ttatgcctgc tcaacgactg gtcggcgcgt
     6421 gacattcagg cctgggaata tcaacccctg ggaccgttcc tggccaagaa tttcgccacc
     6481 acgatttcgc cctgggtcgt caccctggaa gcgctggcgc cgttccgcgc gccgttcacc
     6541 catcccgcga gcgatccgca acccctaccc tatctggcct ccgccgagaa cagcgccggc
     6601 ggcgcgttcg acatagacct ggacgtgctg ctgagcacac ggcggtcgcg ggccgcccat
     6661 accgagccgg cggtactgtc acgcagcaac ttcaaatacg catattggac cattgcgcag
     6721 atgatcaccc accacaccgt cagcggctgc gatctccgcc ccggcgatct gctgggaacc
     6781 ggcacgcaat ccggcccaca agccggccag gaagggtcgc tgctcgagct gaaccagggc
     6841 gggaagaaac cgatagcgct gccctggggc gagacacgca agttcctgga ggatcacgac
     6901 gaagtcattc ttcgagccgc ctgccgcaag aatggctatc ccaccatagg tttcggcgag
     6961 tgcgcgggca ccgtgctgcc aagcctgccc acatgatgat acaaggcgaa tactcctcga
     7021 acggcttggg aaaactgttc gaaccggatc aaatacccgc caggtgaatc ctccgaggct
     7081 tgttcgttcc gggccaacct gggccggaac gcatcaggcc aggaccttgc cgggattcat
     7141 cagtccgtcg ggatcgagcg cctgcttgat cttacgcatg atggccagtt cgaccgggtc
     7201 tttgtatttg ggcagcgcat cgcgcttgag ctgccccacg ccgtgctcgg cgctgatcga
     7261 gccggcgaac cggtgcacgc tgtcatgcac cagttcgtac accgcgtatt ggcgatccaa
     7321 cagttcctgc tctgtgaagg cctggccccg cgccacgttg tagtgcagat tgccgtcgcc
     7381 aaggtgcccg aagatgacgt gctcgatgcc cgggaacgcc gcctgcagcg cggcgttggt
     7441 gacccgcacg aattcagcca tgcgcgacac cggaatggac acatcgtgct tgatgctctt
     7501 gccgaaggcc ttttccgcca acgggatgct ctcgcgcagg tgccacagcg ccttgctctg
     7561 ggccacgttt tcggcaatga ccgcgtcggg caccaggccg gactccagca tgccccccac
     7621 cacggcctcg aagcgctcgc gcgcgtgttg cgcgctttca ctgtcggaca actccagcag
     7681 cgcgtaccag ggaagctgcg ccgaggggcc atcgaaagga atgcgctgtt gtgggtagca
     7741 ttcaaccacg cccttcagac aattgccggc gatgagctcg aaccccgtca gtgaggcgcc
     7801 gaaccccttg cgggccaccg acaacacctt gaccgcgtcc tccacggacg acaaccccag
     7861 cagcgccgtg cattgcgcga ccggcaatgg atacagcttg agttcgaccg cggtgatgat
     7921 gcccagcgtg ccctcactgc cgatgaacag gtcgcgcagg tcatagccgg tgttgtcctt
     7981 gcgcaagccg cgcaggccgt gccagatctc tccttgaggc gtgaccacct caagccccag
     8041 cgccagctcg cgcgtgttgc cgtatcgcag gacctgcgtg ccgcctgcgt tggtgcccag
     8101 gttgccgccc actgtgcaac tgccctcggc cgccagactg agcggcagca gccggtcggc
     8161 gtcccgcgcc gcctgctggg ccgcctgcaa tatgcagcct gcctcgacca ccatggtgtc
     8221 gttgtcggta tcgatggcgc gaatccgatt catgcgcgtg agcgacacca caagcgcggt
     8281 gccgctgcca tcgggcgttg cgcccccgca caggcccgtg ttgccacctt gcggcacaat
     8341 gggcacccga tgcgccgcgc accacttcac cacatccgcc acttccccgg tcgaacccgg
     8401 ccgcaccact gccagcacgc ggccatgata gcgatcacgc caatcggtgc agtagggttc
     8461 ggcatcgccg ccggtcagca cattggcctt gcccactatt tgaatcaatt cgtccgtttg
     8521 cgccatgtcg ttcgtgcccc ttcggtttca acgcgattcc ggaatgattg tagccccgcg
     8581 caaaactgga acgaccgtga ccattccgcg ataatggcca ccctgcacgc gcacgatgtg
     8641 ccgcctgtgc gcgccaccga gcagaaccca aggaccccca tgcccgccac cccacccgaa
     8701 tccgtcttca aggcctacga catacgcggc atcgtgccat cacagataga tgccggcttc
     8761 gccgcgcggc tcggccgtgt cctgggccga atggcccgcg aacaaggcat cgaggcactg
     8821 gtcgtgggcc gcgatggacg gctaagcagc ccgtcgctgg cttcggggct ggtcgacggc
     8881 ctgctggccg agggcgtgga tgccatcgac atcggcctgg tgcccacacc cgtggtgtac
     8941 ttcgccgcgc atgtgctgga aaccggatcc ggcgtggcca tcaccggcag ccacaacccg
     9001 gcggactata acgggttcaa actgatgctg ggcggtcgga cgctccacgg cgatgacatc
     9061 acgaccatcg gccgcgccat gtcggcgacg ccggcggatc accgcccccc cgcgcgcgcc
     9121 ggcactcggc gcggtctcga catcctggac gcctacgtat cacggatatg tcaggacgtg
     9181 aagctcgcgc gccccatgaa gatcgccatc gattgcggca acggcgccgg cggggtggta
     9241 gccgcgcggc tgttcgaaac cctgggctgt caggtggacc gcctgttctg cgacgtggat
     9301 ggccgtttcc ccaatcatca tcccgacccc tcggttcccg ggaatctgcg cgacctccag
     9361 gcccacttgg cgagcagcga ttgcgaactg gggctggcgt tcgacggcga tgctgatcgc
     9421 ctgggcgtgg tggcgcgcga tggcgagatc atctggcctg atcggcaatt gatcctgttc
     9481 gcccgcgacg tgttgagccg acatcctggg gccaccatca tttacgacgt caagtgcagc
     9541 cgccatgtgg ggcgtgagat cgaagcgtcg ggcggccatg cgctgatgtg gagaaccggc
     9601 cattccgtga tgaaagccaa gctggctgaa accggctcgc cgctggcggg cgaactcagt
     9661 gggcacatct tcttcaagga ccgatggtat ggcttcgacg atgggctgta tgtgggcgcg
     9721 cgcctgctgg aaatcctgtc gcgcacgccc gacgcgtcga ccacgctgcg tggcctgccg
     9781 gtgggcattg ccacaccgga gctgaaaatc gccgtgcccg agggggaaca gcacacgcta
     9841 gtcgcccgat tgcgcaccga agcggcgttc ccttccgcca ccggaatcgt ggccatcgac
     9901 ggcgtgcgcg ccgaataccc cgacggcttc ggcctggcgc gcgcctcgaa caccacaccc
     9961 gccatcacac tgcggttcga aggcgacacg ccccaggcgt tgacgcgtat ccaggatgaa
    10021 ttccgccttg cgctgagccg actcgcaccc aatgccacgc taccgttctg atgctggccg
    10081 aggcagcccg cgcacagtcc atggaattcc ccccgcctgg aaacggcgcc gatttgcgcg
    10141 aataatggtt acccgacttc aggctacctc acgccttatt tcttgcgcaa tgattcggac
    10201 cccgccctct atacgatcgg cttcaatgga tgaatagccg attcgcatat gattgccggg
    10261 ctgttcattt ccggcaaaaa acacgtcacc aggctcgaca acgacccctt tgacgtacag
    10321 gtttcgggca agcttacgag catccagatt ttcaggccct gtcacccaca atgccgaccc
    10381 gccctgcgcg ggaaccagca tcagcccggg gatgtgctca gccaaagcca acctcaacgc
    10441 agttgcacgc tgcttataca cgatgttcag ccgcctcacg aacgcttcgt ggtacccatg
    10501 cgcaataaaa ctggccgccg accgttgatt atttgttggc acgtgtcgca tcatcaaccg
    10561 acgcaacgct ctcaactcgc gtatgagatc cgctggcgcc acgacgaagc ccagccgcag
    10621 gccatgagcc aatgtcttcg aaagactgcc aacataaatg acacgctctg ccgaatccag
    10681 gctcttcagc gcaggggagg ggcgacccga aaaattcaac tcgctttcat gatcgtcttc
    10741 gatgatgaca aaatcatcgc gcgctgcatg tgccagcagc atatggcgcc gctccagcga
    10801 catcgtgacg gtggtcgggc attgatgact gggagtgacg taaacgtagt cacaatctga
    10861 caaagcgggt ccagaaacaa ggccctctcc atcaacgtcg agaaaccgca cgtctctgga
    10921 tcgaagagaa aaattattgc gcgcatcggg atagccggga ttctccatgc caaccacggt
    10981 ttttgagtcg atcaacaact cggacaatag gaaggtcgct tgctgcgcac cggtggtgac
    11041 caggatttcg tcttgctcca cccaaatacc gcgcgcgggc agcaaacgcc gctggatctg
    11101 ctcaatgagc agttcgtcgt cacgatcgag gtgatcaggc gcccagtgtc gcaatacgcg
    11161 cggctgcagg gtctcaagca cgcattcccg ccattgaagc gtggggaaca ggctggcatc
    11221 aaactgccca tagatgaacg ggtatggctg attctgccaa tcgaccggtt tttctatgtt
    11281 ctgttgccgc gatggcgtga acatcagccg ccttccccag gcgacgcgct cccgccccgc
    11341 gctgtcggtt tgcggcttcc ggacgtcggg ctgaatggat tgaccttccc cggccacgat
    11401 gtagccgctg cgcggatggc caaccacaat cccactttcc gtcaatgctt gtaatgccgc
    11461 catcgcggtg gtgcgcgaca ggccaagttt ttgcgccaga aaccggctgg atggcagcgc
    11521 ctcaccgggc agaatgaaac cctcgatcac cgcgtgcatg agcgtgtcgc gcagttgccc
    11581 ctgcaaggta ttggtttgcg ttttgaatcg cgcaaaaagc tcagaccaca ttagatcgtt
    11641 ggtgcgagtg gattgtttca tcagcggtgc gcacgtccga aaaagcggga aatttctggc
    11701 cttattggtt gatcgtatct ggtatgacgg caaatatcca ccgtcacgta tcattttctc
    11761 catcaagcag acaggagaca aacgtgcgct acatcaagtc ggcggcaaat caaaaagccg
    11821 aggccgagaa ccggacaatc cgccagcaag tggaaaccat gctgtccgac atagaaaaac
    11881 gccgcgaaca ggcggtaatc gactatgcgc gccagttgga tggctgggag gacaagtttg
    11941 ttctttcgcg ctcaaagctc gcgacactta tccaggaagt tcccgatcaa ctcaagcagg
    12001 acatccattt cgcgcacaga caggtcacgc ggttcgcaca ggcccaacgc gacagcctgt
    12061 caggatttga aatggaaacc gaaccgggcg tgcgattggg ccagcgaatc gttcccgtgt
    12121 cagccgcggg atgctatgtc cccggaggca ggtacgcaca cgtggcatcc gccatcatga
    12181 gcgtggcgac tgccaagatc gcgggggtgc aaggcgtcgt ggcaagttca cccccgcggg
    12241 gccacagcat aaacccagcc acggcttatg ccatggaaat ctcgggggcc gacatcattc
    12301 ttgaaatggg cggggtgcag gctgtggcaa ccatggctta tggcctgttc gactgtccgc
    12361 aggccgatat catcgtcggc ccgggcaacg cttacgtcgc ggaagccaaa cgaatcctgt
    12421 ttggaaaagt ggggatcgat gtctttgccg gaccaacgga atcggccgtc atcgcggacg
    12481 aatccgcgga ccccatgacc atcgccatcg acctcgcttc ccaggctgaa catggaacca
    12541 actcaccggt ctggttgttc accacgtccg aatccatcgg tgaggccgtc tcccggctga
    12601 tgcccaaggt cgcgggggac atgcccaacg ccagtgtcgt caatacagcg tggcacgatc
    12661 acggggtgat cgtgttgtgc gagtcacgcg aagaaatggc gaaggtgagc gaccaatacg
    12721 cgccggagca cgtgcaagtc ttggccgacg atctcccgtg gtggaaagaa aatctgcgca
    12781 actacgggtc gctgttcctg ggtgaaggaa gcacggtcag ccacggggac aagtgctcag
    12841 gcacgaacca tattcttccc accagcgggg caggacgata tagcgggggc ctgaatgtcc
    12901 agaaatttct caaggtactg acataccagg aattgtcgcc cgaagccaac aaggcgttca
    12961 gcgccgtagg gtcacgcctg tcccgggcgg aaggcatgga ggggcatgcc cgggcgtgcg
    13021 actggcgcct gcacaagtat tacccggata ctcgctggga cttcgatgtc tacaaacaag
    13081 aacgctacgt ctgatctggc tgattcgcca tggaaaaatt ccgcaaatcc tttacccagc
    13141 aggaaccgat tccagaatcc gggatcagtc gcgccgtcga agtcatgcgc agtggccgac
    13201 tccaccgcta taacgtcaac gaaggggaaa aatccgaagc aaccatcctg gaagaaaaat
    13261 tcgcccacta tcttggcgtg cgtttctgcc tggcgtgcgc ttcgggcggc tacgccctga
    13321 caacagcatt gcgcgcgttc ggcatcaagg ccaacgagcc ggttcttacc aacgcattga
    13381 cactttcacc cgtacccggc gccatacatt cggcaggggc ccatgcggta cttgtggaca
    13441 ccacagagca gcttgttctg gatctggacg atctggacag gaagcttgcg tcaacctcgg
    13501 caagactcgt tctggtctcg cacatgaggg ggcacatcgt ggatatggat gcattgtgcg
    13561 ccatcctcgc cagacacggc gccgccctca tagaggactg cgcacacacg atgggagcat
    13621 cctggaacgg ccgccccagt ggcacttttg gcatcgccgc ttgtttcagc agccagacat
    13681 acaaacacat caactctgga gaaggcggat ttctcaccag caatgacccc ggcctgatgg
    13741 cgcgcgcgat catactgtcg ggttcctaca tgttctacga tcatcacact gcgggccccg
    13801 caccggaggc gtaccaggaa acacgcatgg tgacgccaaa ttgctcgggc cgcatggaca
    13861 atctcagggc atccatactg atacctcaac tggaagcgct ggacgatcgg gttgaacaat
    13921 ggaatatccg gtatcgcgcg ctcgaggcgg gactgattga cgtccccgga ttgaaactcc
    13981 cctgcagatc gccgaaagaa cattacgttg gaagctccat acagttcctc atacccggaa
    14041 tcaccgcaca aaaagccgaa gaactccttg ccatctgcca gcgacaaggg gtggtgctca
    14101 aatggtttgg cgcacccgag ccccggggct acaccagcca ctacgcaagc tggcgctatc
    14161 tgaccccaca aacccttccc cgcacagacc aggttctgaa cagcctgttc gacatgcgca
    14221 tccctcttac tttcagcgtg gaggattgcc atcttctcgg aaatatcatt cggaacgtag
    14281 tcaaagccct gcatcactca taacatggca tcgccaggag acaaccatga acaggtatat
    14341 aaagatcatc gccgcattgg gcgtgacggt cgggatcagc accgtcgccc acgctgaaaa
    14401 actcatcatc gggacctttg gcgatcccac gcccgctcag gccgccatcg cccaagggaa
    14461 gttcgccaaa gccacgggat ggaacatcga gtggcgaaaa ttcgagtcag gcgccagtgt
    14521 gatttcggca atggcttcgg gggatgtgca gatttcagag ctgggatcct cccccctggc
    14581 gatcgccacc agccaggggc tggatctcaa gttgttcctg gtctcgtttc ttatcaacac
    14641 atcggaatcg ttgattgtcc gaaacgggac cggcatagac aagccagcgg atctcaaggg
    14701 caaggtcatc ggcgtgccga taggctcgac ctcgcagttg tccctgacgg gggcgttgaa
    14761 acattggggc atcagcgaaa accaagtccg catcctggga atgacaccac aacaaatcaa
    14821 tgcggcctgg gcgcggaaag acattgatgc cgctttcgtc tggaacccgg ttcaaagcga
    14881 gcttctgaaa aacggcaagc gcctggtatc ggccggccag gtggcgaagt ggggctatcc
    14941 aacattcaac gggtgggtcg caagctccac attcgtggcc aagcatccca aaggcgtggc
    15001 tgcgttcgca aaagcaatga atgaggcaaa cgccgcctac ctggacaaca agtcgaagtg
    15061 gacggctgat tcagaaactg tgaagcaaat agccaagcga acgggtgcgc agccagcgca
    15121 ggttccgtcg attctacagg gcttcacctt cctgacggct tcggaacaaa cagcctggct
    15181 gagcactcac gctgctgacg ccttgaagaa gaccgcgatc tttctgaaaa agaaccatcg
    15241 aatcgacacc gtgaaaaaca attacggtga attcgtggac accgccgtgg ccaaatccgt
    15301 tcagtgacgc gttgccactg gcaagactgc gcctcgccag gcaaggcacc aaagcttcga
    15361 gcaggctaat cagaacacgc tatcagcgca tcatagatgg ccagatgcgc ctgctcgacc
    15421 ttgcgtacgt cgaattcccg ttcggcaagg tcgcgccccg cggcacccat cttgcggcgc
    15481 aacgccgggt cagcggccag cctggccacc gcctcggcca gcttcccggc gtcgcgagcg
    15541 ggtaccagca aacccgtgac gtcggcgtct atggcatcgc ggcaccctgg cacgtcggtt
    15601 gtgacaacgg ggcgcgcgca ggcggctgcc tcgatcaacg acttgggcag gccttcgcga
    15661 taggaaggca gcaccgcgat gtgggcaccc cggtacaacg ccgccacgtc atcctgttcg
    15721 ccgaggtaat ccacgacatc ttcccgcagc caggccgcca gcgtgtctgc ggacacgctg
    15781 gccggattgc ctggatccac gctgccggcc agcacccagc acacgccagt gcgccgggcg
    15841 gccgacaggc gcgccgcctc gacgaattcc tccacaccct tgtcgcgaag caggcgcgag
    15901 gccatgaccg cccgcaccgg gctgtcgggc tcatcggatg ccgcgaagac gtcgagatcc
    15961 acgcccgaac cacgaatcag cacagactgg tcctcgcgca ccacgccggc atggcgcagg
    16021 acatcccgat ccgccgtgtt ctggaagacg acccggctgt tgcgatggcc gagggccagc
    16081 ttgtacagga acaccgcggc cgaacgcagc ggatcgaacc ggccgtcgcc ccgcatgaac
    16141 acgtagccca gcccggagac ggccgcgacg aaggccggca cccgggccag acgcgcggcg
    16201 atgccgccat acaggacagg cttgatggtg acggcatgca ccagatccgg gcgcagccga
    16261 cgaaacaggc gccacaaggc aaaaagactg tgcagttccc gcagcgggtt cttgccgctg
    16321 cgggacatgg gaatgacgtg atgcgccagg ccgtgggaca cgatgcgcga taccgaggga
    16381 ccatccatgg tggcgacatg cacctcgaaa ccggcgcgct tcgcagccag ggccaacggc
    16441 aatcgatgag acaggaagaa ggcggggttg ttcaccacga acaacagatg gcgggtgtcg
    16501 gggtgctggg ttcgttgggc atcgctccgt cgcacggtgg ggaaaccgtt cgcgatgcgc
    16561 cgccacgcct gcccatacgc ccgcaccatc acatccagac cgaagcgacg ctgcacactg
    16621 tcccgcgcac gcgccgacat ccgcgcggca tcatcggtgc cgaatacgtc cagtgcggcg
    16681 gagatcgcgc gcgccagcgc ctcggcgttt gacggtggca ccacccagcc attttcctcg
    16741 acaatgacgg ccgcatcccc cacattggtg accacgcata gcgggccggt ggccatggcc
    16801 tcggccacca cattgggaaa gccttccgca caactggaca acacatgcac gtccagcgcg
    16861 cccatcacgc ggggcacgtc gtggcgctgc cccagcagca ccagccggtc acgcacgccg
    16921 tggcgctcgg cgagcgccgt cagctctcca ttcgacgcct ccatgccctg gccgaccaga
    16981 acacagcgca accgcgcgtc acgcttcacg cagcgagcca gcgccgcgaa cagattggcg
    17041 tgatccttca gcggattcca gcgcgccacg ctcccgatga gcggcgcctc gccgggtatc
    17101 ccccactccg cccgcagggt acgacgtgcg tccgcgtcgg cgtgccagcg cgaaaagtcg
    17161 taaccgttgg ggatgacctg gaatttcgcg gaggcatatc cccattgttt atggcgctgc
    17221 gccgcatctt gcgcgcaggc gatgatggcg gccggcaccg cccccgacag gcgtgcgcac
    17281 acccaggcga cgatccgtgc caggcggctg cctttgccca gattggcgcc ggagttgcgt
    17341 atcccccagg caacggcgcg cacacccgcg agacgcgcga ccacgccgcc aagcagatca
    17401 gcgtgataca tccaggtctg caccacatct ggcgattccc ggcgcagaaa ccggtgcaac
    17461 gcgaccagcc cgcgcaaggc cgccagaccg cggctcatgc ccaggacggt aaccggcacg
    17521 cccatggcgc gcaagcgcgg accaaagacc ccttcgtcgg tcagagacac caccgtgtgc
    17581 cgcaccgact ggcccggcgc cgtcaccaac cggtacagca ccatctcggc gccgccctgc
    17641 cccaggccgg aaatgatgtg tattacatgc agtggcctat cctccgcagc catggcgtca
    17701 ggatcattca gcgcggtcat gagccggcac ccggcacggc cacgccggcg cgcgccatca
    17761 ggtcatccca ggcggccagg acggccggca agccgtagcg ttgccgcacc gacaacgccg
    17821 cgccgcgtgc caacctgtcg cgcaacgatt catcgcccaa taattgccgt aggcccgcga
    17881 ccagcgcatc cggatcggac accgggaaca gcaaggcgtc ttcgccgtcc tgtgagattt
    17941 cgcgcgggcc gctcggacaa tcggcggcga tacacggcag gcccagcgcc atggcttcta
    18001 gcagcgcatt gggaaaacct tccaccgaag acaccagggc aaaggcgtgg acgcgggata
    18061 gctccctcca gggctctggc gtgcgcccgg gcaggaatac ccgatcgctc aggcccacgt
    18121 cccgtatttg attcgtcaag gcctcccgca tgggtccgtc gccccatatg acgagatccc
    18181 aatcagggaa ttcgccggcc agctgcgcaa acgcacggat gagccaatcg aaccttttcg
    18241 ccggcacgaa ccgccccatg gccgcgagcc gtcgccgccc gcgcacatcg cgcacacggg
    18301 cggcgagcgc catgtcgggc agttcgggag gcaggggatt ggccacgacg cccagggcgc
    18361 gcatgcgcgc cactttctcg cgcagcgcct gaacgctatc ctgggcctgc acggtaacca
    18421 tgttggccca cggataggtg acacgccgca accagccaag caatcgcccc acattgtcgc
    18481 tggccgcggg gttggtgcgc tcacacacga tgatgggaac gcgcagcccg atcgtgctca
    18541 gcaaagccat cacgttcacg ttcgtcagaa acgaaacgat cacgtccggg cgccgttgac
    18601 gcaccaggcg acgcaacacc cgcagcttgc gcagccccga cggcgcggca ccgcccgccg
    18661 ggccgatatc agccagccag cacagctcga cttccgaagc caggggataa aagcacgtgc
    18721 cccgccccgt gaaagtggga accagcgtga ccgagtcgcc ccttaccgcc caggcgctgg
    18781 caagcgtggc cgcgacccgt tcggcgcccc ccgcatgcat agagctgacc agcagcatga
    18841 tcttcacgtg gcctccccgc gcaccgcgcg ccggctttcg tccagccacg cctgcatcat
    18901 cagcacgctc cacaggtgcg tgctccagtc gcgcgcgccg gattgatgtt cctgccattt
    18961 gcgcagcacc ggccgcgcct ggaacacgcc gtcatcgcgc aggcgcactg gatccagcag
    19021 agccgcgccc cagtccctca acgggccccg caaccagtgt gcgagcggca cggagaatcc
    19081 cttcttgggt cggttcacca tggcctccgg cacatggcgg cacagcaacc tgcgcagcag
    19141 gtccttgccg tggccatccc gcaccttgta ttcatgcggc aggcgtcggg cgaattcgaa
    19201 gacacggtgg tcgatcatgg gcacgcgcgt ctccaggccc acagccatcg ccgctcggtc
    19261 gactttcacc agaatgtcgc ccggcagata ggtgaccgtg tccagcagcg tcatgtgatc
    19321 gaaaaacgaa tccggcgagg gttgatcgaa caaactgtcg ggatgcccgg ctgcgacgag
    19381 cgcctgggcc gggtctttcc agtacgacac gaacgattga tagaagtgcc cggcgtgccg
    19441 ggccgacagc gcgttcgaca aacgctcgac acgttcgcgc acacgcccgc ctggcatcat
    19501 cgccgcggcc gtgtggccta gcacacccgc cggacggcgc acgaggccag gcagcgccga
    19561 acgcctctgc caccaacgct gcgcccggaa ataacgcgag tatccgccga acagctcatc
    19621 gcccccgtca ccggacaacg ccaccgccac gtgttcgcgg gccatgcggg tcaccaacac
    19681 agtgggaatc tgcgacgaat cggcgaacgg ctcatcgtag agattcgcca gtcgtggcac
    19741 cacatcgagc gcatcgcgcg ccgacacata cagctcggtg tgccgggtgc cgagatggcg
    19801 cgccacttcg cgggcatagc ccgcctcgtc gtaggctggg tcgtcgaagc ctatcgagaa
    19861 cgacctgacc gcgttgccgc cttgggcctg catcagtgcg acgatgaccg acgaatcaat
    19921 gccccctgac aggaaggccc ccagcggtac gtcggacagc atctgccctt tcactgattc
    19981 gctcagtacc tgttcgagcg catccacggc ctggtcgtca gagttgaatg ccaggcgatg
    20041 ttcgatcgca tcacgcgcca cggcctgcgc cgaccaatat tgaaccggat cgggcagccc
    20101 gcgctgacgc aactgcgcct cggagaccgt catgaacgtg cccgggcgca gcttgcgcag
    20161 cgccttgtag atgcaccagg gcgcggggat gtagttgtgg cgcagcagca gcgacaaggc
    20221 cgacggatcc agtgtgggat cgaagccggg cacatcgacc agcgccttga gctccgaggc
    20281 gaagacaagc cgcgatccgg catagcccag gtacaacggc ttctcgccca tgcgatcacg
    20341 ggccagagac agtgtgcggt cataccggtc ccagagcccc agcgcgaaca tgcccacggc
    20401 gcgctcaagc gtcgccgcga taccccatgc cgcgagacag gccaggaggg tctcggtatc
    20461 ggaatgcccg cgccaggccg gcgcactgcc ctcggcccgc aatctgtcgc gcaagtcgag
    20521 atgattgtag atctcaccgt tgagcaccag cacgaaacgc ccggaagccg acaccatggg
    20581 ctggtgcccg gcgtcggaca ggtcctggat ggccagacga acatgaccaa ggccgagctc
    20641 ggcctcgcgg tcttcccaat agccgcggct atcggggcca cgatggaaga tggccgcgca
    20701 accgcgttcg atgaccgcgc gccgatccgg caattcaccc catacgccga ctattccaca
    20761 catttcccgc ttactccccg cccccggcca cagtggaccg gtggtctcga tcaccttcga
    20821 agacatgatg ccacatggcc atgacacggg ccgtggaaaa ccggtcgcgg acatcggtcg
    20881 cgcgccgcgc gtaggccgcg cgcaaatcgg gatgggccat caggttggac agatgggcgg
    20941 ccagcgcctc ccaatcctcg gccggccgca ccagcacgcc gtcgatgccg tctcgcacga
    21001 cttcgcgcgg cccggtctcg caatcgaagg caaccgccgc gagaccgctg gccatcgact
    21061 ccagcaaagt gttcgacaac ccctcgacac gcgaactgag cacgtacagg tccgacgact
    21121 cataccactg gccgacgttg ccagcccgac caggcaggct gacgcgatcg gacaggccgg
    21181 acgcgtggac ctggctttgc agcacgtcgc gttgcgtccc ctcccccaga atgacgagat
    21241 cccagtcggg gaaaagcgcc gcaatggatg cgaacgcgcg aatcagcaga tcgaacccct
    21301 tttgcgggtg caggcgcccc accgccagca ggcgatgacg acccgcgcga tcgggaacgg
    21361 ccagcatggg ctcagccgga tcgagcggcc agcgcacggc attgggaatg accgacagcc
    21421 gcgaacccgg cacgttgcgt tctatccacg ctgccgttcc cttggtgagc gccaccacgg
    21481 ccgccgcccg gggataggcc cagcagcgca ggcgcagcca catgggcggc agactctggg
    21541 atggcggatg agtgtgttcc gtggcgatga cacggcacgg caacctgcgc gccgcgatca
    21601 ccgccagcac cgacgacgtg gtcatcatgc ccaggacgat atcggggcgc tcgcgacgaa
    21661 tgaggcggcg caaccggaac acccgccgca gattcgccag cagcccccgg atccgccccg
    21721 ggctgccgcc agccgtgccc agcaccacac gccgaacgtc cggatgcaac gcataggcgt
    21781 caccttcggc accagtctgc gtgaccacgg tcacccgata gccgcgcgac acccaatgcg
    21841 cacccagatc ggcggccaca cgctcggcac cgcccccctg gagcgagtgg atgacgatca
    21901 tcaaatggcg ggagacggaa gaggctttcg agaccatgct tgcagccgcg agaattcaca
    21961 cggaaaagat caatgaggac ggtgcgtcga accggatgcc gccttcccgc cggacaccgc
    22021 cggcagacgc agcacgacga ccacataaca actgtacgat agcgcgtaca tgacgctcgt
    22081 ggccaacatg atgccggccg cacccatcga gggcgccagc acgtaattca tgatagcttt
    22141 cagaacaaaa ttggccacgg cgatggccgc catgatggca taacgattct ggctggccag
    22201 tagctgcacc aggatcaaca cgccaaaata gaacggcaat tgcaacaacc cccagcgcaa
    22261 cacatgcgcc accacctgcg tgttctgggc cgtgaacgcg ccgcgctgga acaacagcgc
    22321 caccccccag ggtgccagca accagcccac cagcgcggcc agcgccccgg ccagcagcat
    22381 cagcgccgac cacttcaacg ccatggcgcg ggcatgatgc gcatcaccgc gcccctggat
    22441 atcggccagc acaggcaagg ccgcgcgccc gacagacgcc gcgccgatgc ccagcaccaa
    22501 cgacagcagg cgcgccgcat agcccaaggt ggcgttcgca ttggaaccca gggtggccgc
    22561 cgtgtactgg tcgatgggcc ccacgaaact catggccacc tggccgatca gcatcacccc
    22621 ggcagcgcgc gtcaggtcgg gccagcgttc ggagcgacag gtcaaccggg gtcggccgcg
    22681 gaagccatcc gcccgtgacg ccagccagaa cagccacact gactggacca ggtagccaac
    22741 cagcgtcccc cacaacagcg gccccacgtc acgcaccgag cccgccagca gcaccaggac
    22801 gagaatggag agcgccggca cgctgtcgag cagcgtgttg atgtggcgct catgcgcgcg
    22861 cagccgcgca ccgctgatcc cggtcatcaa ggtcaggaag gccgccgggg cgaacgccag
    22921 ggtcagttcg cggctcatgc gccgcgcctc gtccgtcaag ccatgacccg ccacatccag
    22981 gacataaggc caaagcgcat aaatgagcac ggccagcacc acccccagca ccatcaccca
    23041 gccttgcagc tcgcccacga accgggacca gtcggccttg tccccatagc ggacacgcac
    23101 cagaaccgga atcagcacga ccgccagcac ccccacgatg gtcacgggga gccagttcgc
    23161 catggtcatg gtgaactgat aggcatcggt ggcgttgctg acaccatagc ggtacgccac
    23221 cgccatttcc ttggccgctc ccgcgaactt gcctaaaagc aggaagaaag cgacacgcgc
    23281 cgccccccgg gcgatgcgtc ggtggtcgga atgcagggtg gaaaggcgcc gtgcgagggt
    23341 tttcaagtca acgcaggaac tgcgtgtacc acggcatggc gacttcgagc ccttgcacga
    23401 tgctgtgcat gggctcgtag ccgagcagac gcttggcctt gctgatgtcg gcttgcgaat
    23461 gccgcacgtc gcccgcgcgg aaatcaccgt ggatgggcgg tttgtcgtac acgatgccgt
    23521 tgttggccag cgcctgggcc agatactcga aaagctggtt caggctcgtg cgcgcattga
    23581 acgccacgtt atagacctgg ttgacgccat cgggctgggc cagtgccgcc aggatgttgg
    23641 cctgcacggc gttctcgacg tagcagaaat cccggctggt ctcgccatca ccattcacga
    23701 caacgtcctc accgcggatc atggccgcca cccattttgg aatcacggcc gcatacgcgc
    23761 cgtccgggtc ctggcgcttg ccgaacacat tgaagtaacg caagccgatg ctgccgaaat
    23821 tgtacgaacg agcgaagatg tcggcataca gttcgttgac atacttcgtg atcgcatacg
    23881 gcgaaagcgg ccggccgatc tggtcctcga ccttgggcaa gccgggatga tcgccatagg
    23941 tggagctgga cgccgcatag acgaacgact tcaccttggc atcgcgcgcg gccaccagca
    24001 tgttcagaaa gccagcgatg ttcacgtcgt tggtggtgat gggatccgcc agtgaacgcg
    24061 ggaccgaccc cagtgccgcc tggtgcagca cgtagtcgac ccccggcatg gccgcgcggc
    24121 aggcgtcgag atcgcggatg tcgcctttat ggaaggtgaa attcccccat tgcgccgccg
    24181 acaccgtctt ccgcacctcg tcgagattat gctgatagcc ggtggagaaa ttatccaacc
    24241 ccacgacctt ctgccccatg agcagaagcg tttcgagcag attcgagcca atgaagcccg
    24301 cacagccggt gaccagccat gttcgaggag ccgcctgcaa tccggcgact acttcctggt
    24361 aacgagtact catgtcaaca aaccctttta tgcctgccta gtgtgctgtt tggtcaaacc
    24421 gaatttgaat gcacggatca acctaacagc acacttggcg atcacagacg cagatcggcg
    24481 tcggccgcgt cgaacacgta cttcaggtcg tagagcacgt gctcgggttt gcccagcgcg
    24541 tgaatggcct gcgcccccat ggcagcgaac tgatgatgcg ccaccgccac cacgatgccg
    24601 tcgtaggcgt gcgactcggg cgccgccacc ggccggatgc cgtactcgtg ctcggcctcg
    24661 tcggcgtcga tccacggatc gtagacgtcc acccgcacgt tgtactcgcc cagctcccgc
    24721 acgatgtcca ccacccgcgt gttgcgcagg tcggggcagt tctccttgaa cgtcaatccc
    24781 atgaccagca cccgcgactc gcgcacctgg atgctgcgtt tggtcatggc cttgaccagc
    24841 tgggccgcca cgtacccgcc catggagtcg ttgagccggc gcccggccag gatgacctcc
    24901 gggtggtagc cgatggcctg cgccttgtgc gtgaggtagt acgggtccac accgatgcag
    24961 tgcccgccga ccaggcccgg acggaacggc aggaagttcc atttggtgcc cgccgcctcg
    25021 agcaccgcca gggtgtcgat gcccatcttg ttgaagatga gggccagttc gttgatcagc
    25081 gcgatgttca cgtcgcgctg cgtgttctcg atgaccttgg cggcctcggc cacgcggatc
    25141 gacggcgcct tgtgcgtgcc ggccgtgatg atctgcttgt acagcgcatc gaccagatcc
    25201 gccacttcgg gcgtggagcc ggaggtgacc ttcttgatgg tgctcacgcg gtgggacttg
    25261 tcgcccgggt tgatgcgctc ggggctgtag cccgcgtgga actcatcatt gaagcgcagg
    25321 cccgacacgc gctcgagcac cggcacgcag acctcttcgg tcgcgccggg atacacggtg
    25381 gattcgtaga tgacgatgtc gccacgcttg agcaccttgc cgatggtctc gctcgcacgc
    25441 accagcggcg tcagatcagg ctgcttgtat tcgtcgatgg gcgtgggcac cgtgacgatg
    25501 aacacgttgg cctgcgcaag ctgcgacgga tcgtcgctga acgacagttg cgcagcctgt
    25561 gccagctccg cgggttcgac ttccagcgtg tgatcccttc cctgcttgag ctcggccacc
    25621 cggttcgaat tgatgtcgaa accgagaacg gaacgcttct tgccgaattc aaccgccagc
    25681 ggcaggccga cataccccaa accaaccacc gccaatttga catcttggat ctgcaacatg
    25741 gatctcctgc gaacttgagg gaaggagcgg ccggcacacc cgtgccatgc tccggttttc
    25801 acttgtggcg attatcgcgc cccaaggagg gtaataacaa ccaggatgga cggcgccagg
    25861 tcaccagccg gacgtatagc cagatgtatg cggccgaaaa cacgaccagg ctggcgcata
    25921 gcatccaggt gttgtcccac cagatggtgg cgggcaccag cccgatcagc gccagcaccc
    25981 acagataggg cgaggtcatg gcgttgcaca gcgccggcag ggcgtggcgc cggtcacggc
    26041 taaaggtcac ccgcaccagg cgacggaaaa cgagttgatg aagatgcagc gcatccggct
    26101 gatcgaccgg taccccgcgt ttgaagcggc ggcgccagat ggagaacaac gtctcgaata
    26161 cggggtagaa caacacggcc agtgcataga acggcgacac gctggggttg cgcaccacca
    26221 gttgcaccgc cagttcggcc aggatgaagc ccacgaaata cgcgccccca tcacccagga
    26281 agacgcgccc gaacggaaaa ttccagacca ggaagcccag tgtggcggac gccagcgcgg
    26341 cggcgatcaa aaaaatggga atatcctgca cctgcaaggc gaccaaggcc agcgacagcg
    26401 acatcagcgt ggcgatcatg cccgccagac cgttcatgcc atcgatgatg ttcagcgcat
    26461 gtgtgcaacc gcccaccgcc accatcgtga agatcagcga caccggccag aaagacagca
    26521 gccagtccac gcccgagaac cccacgcgcg acaccccgcc caacagccac caggcgatgg
    26581 ccgccgacac gaacgcggcc agcaggcgtt tgccggagcc gatgtccttg gtgatgtctt
    26641 cgagcaaacc cgccacgaag accggcatcg cggcgatgaa caggacgggc cacagccaag
    26701 tcagtgtcat gttgctgggc cccagcacca gcaagcccgc caggctgccc gcgacgacgg
    26761 caagcccccc cacacgcggc gcggcgcgcg cgtgcgatgc ctgaggcttg tgcagatcat
    26821 tgtcgccggt gaaccggcca tgccagcgtt cggaaacaac gatcagaccg cccaccagga
    26881 atgcgacaat acagatatat agccaggaga acgaactcca gacctggggt ggcgtcacag
    26941 agcctccaag tgcaaatggt gattgatgga tattatcggc cgaaccggta acatattcag
    27001 ggaaaacccg aaaaaaaggt gcaacaagac atgatgtcgc cggatggcga acatcgcaag
    27061 gaaatcctga cacccacttc cgacaagccg gcccgcgaat cggggagccg ttgagggaac
    27121 ggtcaacaac gtgacgccac aaccatctgt gcggaacccc tacggattct gcatggataa
    27181 ggaagctctc acaagaaata caaaaaaaaa gcccgagatc caaagatccc gggctaaatc
    27241 caccaaagga ggagggtgga ggagacaacc ttggcatgtc gcgctacgag aaccctgggg
    27301 ttcgaggccg gtgattgggg ttttcccgct gcaatcacgt ttcgacatcc gataagcaaa
    27361 ttatataaag actttttgtg cgttgcaagg tagagatcga aaaaaattgc aaattccgtt
    27421 gcttgtggct ttttcgcact ccgccctctg tgttgttcct gacgcccctc ccaggagcgc
    27481 caggcaatga ccgggttctc ccggattgta cttcgttaca acatcccgca gtaatctttt
    27541 gccacgctgg actgcctgtc agagccagta ggccgtcgtc gtcatgattt tggccatgca
    27601 tttcatggac agccgcacgg gcgccggcaa agggcccgcc ccatggcggc gggcggtatc
    27661 gccgtggccc gcctcgtcat cgcgcatctg ctccacgatc cggcgcgaac gcttgtcgtc
    27721 ggcgggcagc cgtaccaggt gggaatccag gtgatgttcg acctgggctt cggtttcggc
    27781 catgaacccg agattccgcc ccaccccggc gcgcccggcc agcgtaccca gcgcgaacgc
    27841 gcccgcatac cacaacgggt tcagataact gggccggctg tgcagttcgc ccagtcgatc
    27901 acggcaccac gccagatgat cgacctcttc cgacgcggcc cgagccagca caccgcgaac
    27961 ctcggcatcg cgacagaaca gggattggcc ccggtacagc gcctgggcgc agacctctcc
    28021 cacatgattg acacgcatca gagccgccgc atggcgctga tcggacggcg agaggctttc
    28081 atgcgcctcc ggccgcccag ccgggtttcc ccgctgagcg cgcgcaacac cccccaacac
    28141 ccgcaacgcg cggtccgcct cggccagaag ccgatcggga aggttttccc gcctgaaagg
    28201 tatcgattcg tttgacataa atgtattccc gccggccatg cccggatcat cgacatcgga
    28261 tccggcatgg cgcgccaaca agtcaagtat aagacctggg ccgctcggcg gcacacctga
    28321 aggcccgggc cgggtcaggc cgatgacccg tcctccggct gtcccgcttc tcccgcgccc
    28381 gcatcaggta tcgcgcaaga acacgcgcac gcgccgccac gcggccaaca accacgccag
    28441 tggcagaagc acggacagcg ccaccagccc catcccggcg acctgctgat cactggccag
    28501 gtaaaagttg cacagtcgcg atgggatgtt ctgatacaca atgcccacca ccgccgccat
    28561 ccagcaaaag ttcccgagaa agaacagtcg aatgacaggc ttggcccgcg ccagggcgtc
    28621 gcgcagcacc agcccgatgg caaacagcga gatgaacttc cacgcatccg catacggcga
    28681 cgacagcact tcgtcgagcg cccggggaat catccagaac gcgccggcga aactcgccag
    28741 caacaacccc ggcacaccgt actcatcgaa ccggcgatac acggccaaag ccttcgatgc
    28801 cgggccggtc ggccgatttc ggccgtgaga ttcaagcccc cgcgcagcca gcgcgccagc
    28861 cgcaacgacc agggggatat gcacgagcat atgcaacgcc atggttctgg tcagggggtg
    28921 ccgcaggatg atcgccacga gaagcagcat cacggaaagc gcgatgcaca cgaagcgtcg
    28981 cgacgaaagc gacccgaagt ggtttgcggc aatcacggct tcaccccgcc ctgcgccggc
    29041 ctcgccggca ccccccaccc ctgtttcgac tccgtccgca ccggcgcgcg ctcaagaacc
    29101 ctttgagcag cctcacccgc agcgaaacgc aaagcctcac cggacgcgcc ataatccacc
    29161 acccgcgtca aacgcccatc gggcgtgatg acatggtagg ccgcgttgtg cacgaattgc
    29221 cccagagggg ccggcacaac gaccacgccg aacgcgcgca gcaagatctc gaggccggct
    29281 cgagtcggcg ccccatagaa ccgccacaca gtcggatcgg cgtgcagatc ccgcgcatag
    29341 cgcgccaagt catccggcgt atcggcggga tcgaaactga tgctgatcag gcggacgcgg
    29401 tcggccaacc cccgacgctc tatggtgtcc tgcagttgct ggaattccga ccccatggcc
    29461 aggcataggg aaaaacagcg ggtatagatg aaatccacga tcgccacgcg gccatcttgg
    29521 cgcaacagcc catgcaacga gccgcccacg gcggacccat tggccagcgc gacgtcaggc
    29581 agcgacctgg ggtgcgccgc gatcgacaac cgtcgcgccg cctcggtggt gaccaccgca
    29641 tagccatcgg tggcaacacc gatcgccccc agcccgatgg cacataccag cgacagcgcg
    29701 acgaccagcc gcagagatct gcggtcgcgc gctatttgag cttgtccagc tcgggattcc
    29761 cgttccacgg cgccgtatgg tccttggtct tggcgcgctc ggccttgact tcgtccacgg
    29821 tgaccttgcc cgcggaattg ccaaagctgg tgcgcacgtg gctgatgacg gcggcgatct
    29881 cggcgtcgct gagctgatcc ttgaacgtcg gcatctgccc cttgtaagtg gcccctttca
    29941 cggtcaacga gccattgatg ccgtgtagaa cgatctggat catggtggac gccttgccgt
    30001 caacccattc ggatcccgcc aacggcggaa acacgcccgg caagccctgg cccgtggcct
    30061 gatggcaagc caggcaatgc gaggtataga gctgggcgcc attgacagcg ccgcccgcgc
    30121 ccgccttggc cacggcgaag tcggccgcga cacggtggtc tcccatggcg gagggctcgt
    30181 cgctgtagtt cagccaaatg tagcccacgg cccagatgaa cagccccgcc agcaggacga
    30241 tcacgtacca ggggactgga ttgttgctct cgaagggctc tggcatttca cgccgctggg
    30301 cccgcgtcga ttccgtcggt gcgttgttgt ctgacattcg ttcttctcca tcgccacgcc
    30361 gccgatgcac cggcgcgcgc atggcgcatc cactatttcc ccagattcgc cggcacgttc
    30421 ttgatcggat acgtgtggtt gagccccagc agatactgga ccagatccag cgcctcgggt
    30481 ttcgcgacca cgaccttgcc caccggggca taattcggcg ggagagtcac cactcgatcg
    30541 cccttgtcgg ccttgtcctt gacttcgaac agaaaaggaa acgaaggcat gatgctgccg
    30601 ggcacgtacg cccggggctc atagaggtgg cccagattcc agtcggcact gggttgccgg
    30661 gccccgatat tgaacagatc ggggcccgtg cgcatggtgc cgagcagatg cgggttgtcg
    30721 tagtaatagt cgccagccac cccgggccgc ccccacccgc gcttgaaatc ggcgggtgtc
    30781 tgcgactggc tgcgcggctg ctggctatgg cagtacacgc aaccgttcgc gatatacaca
    30841 ttgcgcccac gaagctgcgc cgccgtgtac ggcttcagac cctcggccgg tttgacgtgc
    30901 gagagccgca tgtaaggcac gacgaccagt gtggccgtgg ccagcgccag ggtaaccatg
    30961 gcgccactga gcagtttgac ttcgctttcc atgatcaggc ctccaccata gcggcggcgg
    31021 gcgccttgcg tttatggaac agggccggtc cgatgcgtga cgggccgtga cgcagcgcca
    31081 tggccacaaa atggaacgcg aacacaagat ggccaagcgt catcagcgag ccaccgacgg
    31141 tacgcgactc gaggtaagga atcgtcacgc gcaccgaatc catgaacggc ttggaaggat
    31201 cgagcatgtc caggccctgc agaatgccgc cgatggacag gccgatgaaa taaatggaaa
    31261 accccgtgac gaccagccag aaatgcgctg cgacaagctt ggggtatggc cattcccagg
    31321 acatcacgcg tggcatcacg aagtaaatgg caccgaacat gaccagcgtc acgaacccat
    31381 acagccccag atgcgcgtgc gccaccgtga aatgcgtgaa atgggtgaca gtgttgatcg
    31441 accgcagggc ctcgaatgaa ccctccaccg aactgagcgt gtacatcatg ccgcccagca
    31501 cgatgaaccg cagcgtcggg gagtagatca acgtcttgaa atggccacgc attgtcaggt
    31561 gctggttcac cgagaacgcg atgaccggga tcagcatcat catgctctgc acaatggata
    31621 gcgtcaccag ccagcccgga accggcccac cctccagatg gtgtccgccc acctggccat
    31681 aaaagaacgc cagcgcccag aacccgatca gcgacaggtt gtaggactgg atgggccggc
    31741 cgatgatctt gggcaggaaa taatagatcg tgcccagggc gatgggcgtg tagtacagcc
    31801 cgagcacatt gtggccgaac caccagttca tggtggcctg ctccaccccg aagtgcaggc
    31861 cggggatctt ggccaccagg tacaggaccg ggaaccagaa caacgcggcc ccccaatacc
    31921 agaccgacac atacaaatgg tcgactttcc ggttttgcag cgtgtagacc agcggcaggc
    31981 cgatgagcgc gccgcccacc acgagcagga tgctgacctg ccaggggatt tccagccact
    32041 ccaggccatc gttgaaaccg gccgcgatgc tgcccagccc ggcgatcagg cccgcgttcc
    32101 acaacatcgc gcccagcacc gcaaagcgcc cgccgaccag ctccgtcttg agcacgcgcg
    32161 gcaacgccca catgacgacg ccaaaggccg acatgggcgc ccagccatag gcgaccgcgt
    32221 tcaggtgcaa tgtgcggatg cggccgaacg tcagccacgc atattgcgtg agaaattcag
    32281 gggaatgtag tttgatcgaa ctgatcagcc ccgcgaacga ggcgaagacc agccacacga
    32341 tggcgcagga caaaaagacg aacgccacga aggacgtgga tttatccgcc tgcaagcgtt
    32401 cttccagttc gacgcgcagg cgctcgtcac gcgccggatc cgtggctggg atgccgaccg
    32461 cctcctgcag cgacttgcgc tgcgcggcgg tcagggccgg ctcttcgacg tgcccgatct
    32521 ccccttccga gaaaatgaca cgggctccgg caggattggg atcgaaaacc tggtggcgca
    32581 gcgaccagat gaaaatgaac agacctgtca ccgaaatgat gaaggtcaat agaagtatgg
    32641 tgatggtgtc catgactacc ccgacgagat cggaaggcct ctttcccacc ccacgacgca
    32701 cctccgcccg gccacacgga cgcgctgacg tgcgatacgc caccttccgc tgcaaaagag
    32761 gcgaaccgat gggaactgtt gcttgatgct ttacatgagg aatgaatgcg tcgcgccccc
    32821 gcgcgaagaa tgccgctccc ttttaaaacg acatcacgga aatggtacca acacgcgcac
    32881 ctccacggcc tgataccctt acgcagaatc aggttaacgc atttccgcgg aaccgccacg
    32941 ctctgaccgg ccaaagagtg taaaaatacg acgacccgga cgaagcgctg tctgcgctca
    33001 ccacacaaag gaa
//
LOCUS       DASTNK010000062         4676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_415895, whole genome shotgun sequence.
ACCESSION   DASTNK010000062 DASTNK010000000
VERSION     DASTNK010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415895"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1355)
                     /locus_tag="VFR20_04925"
     CDS             complement(6..1355)
                     /locus_tag="VFR20_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0229892.1"
                     /translation="MSVVAPGLTGQPQALGTPENSPSTGKVVKSILAASSGNLVEWFD
                     FYTYAFFSVYFAEQFFAGVGQTAAYMQAAGVFFIGFLMRPIGGYLFGRMSDRYGRKRA
                     MVVSIVMMGFGSLGLAVLPTAATVGSFAPVLLILMRCIQGISVGGEYGSTATYMSEIA
                     PRGRRGFFSSFQYVTLIGGQLLASLLAVIMTHVLGGDQITAGWWRLPFVVGAVAALVS
                     LWLRSGLEETTTSKDRTHRGSGSFAEVLRYPRAFWVVLGITCIGSLSFYVFTTYMQKY
                     LINTGGLNKGLVAEIMTVCLFVFMLLQPLVGMISDRIGRKNSMLIFALGMIILPVPLL
                     SEIGTHQNPVTVGLLILLAMVVLSFYTSISGIVKSEMFPAHVRGLGVGFTYAIGNSLF
                     GGSASYVALYMKNQGIGGVFPWYVVIIAALGLIAIIAMHDSRHHSTLDNPDSSAYGR"
     gene            1621..3756
                     /locus_tag="VFR20_04930"
     CDS             1621..3756
                     /locus_tag="VFR20_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU0229893.1"
                     /translation="MTPTARFPLLRHWRWGLAALACLALILAVGRWSQGQELKLKAAT
                     LHQASEAYVLALRGVVEKYDDLPYVAALHPALRNLLSQPTDHALVDKVNRYLANLQRR
                     TGAEDLYVIDTQGLTLAASNWNTQASFVGQSYSRRPYFEDALQGRRGLFYGIGLTTGV
                     PGLFIAEPVRDAGRVIGVVTVKLSMEWLEQAWSHAVDPVVLQDGRGIVFLSSVPAWLY
                     HTGHTLGSGDLEWLREHSQYGARDHYDRVPWRIERDADMPGLRLQTRLDNRRKDFLAL
                     QTKLPKLGWTLTVTSDLDTVRRARQKAQAIATLIAALLLLGLLYWRLREKRYAEQRQA
                     RIELERRVEERTRELREAEAFRKSMEDSLLVGMHAHDPHGHIIYVNPALCEMVGYRQE
                     ELLGSLPPYPYWHPDDVDQLAHENDPAQGDVAAHGYEARFRHRDGHEVLAMVYTAPLI
                     DAQDAHRGWMSSIVDITEQKQTEARQHEQERRLQLSARLASVGEMASTLAHELNQPLM
                     ALSNFAVAARAMVGHDQPGMLVTALDEIVQQATRASDIVKRARAFINPVQASHETLAI
                     NELATHALALLQPALRRDRVTVQTSLAADLPLISGDRVLLEQVVVNLIQNAAQAMRDT
                     RPPLRRIELSTRLAGSAMEVSVADHGPGVPIDQMEQVFMPFFSTRADGLGLGLNICRT
                     IIEAHGGRMTVINRPQGGADFTFTLPIKS"
     gene            3753..4352
                     /locus_tag="VFR20_04935"
     CDS             3753..4352
                     /locus_tag="VFR20_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0229894.1"
                     /translation="MKDAPLTLYVVDDDEALRRSLLMLLFSQGVPVQSFESGEGFLDT
                     VDMRQPGCVILDLRMGGVSGLEVLERLRVAHSPLVALFLSGHGDIPTALEAMRNGAYD
                     WVVKPDTQRLLEKLPLALAEARARGAALHDWGELTPREREVALLVGLGQPNKEIARIL
                     VPPCSPRSVETHRASIFNKLGVANDNELGRWLASHPWLV"
     gene            4580..>4676
                     /locus_tag="VFR20_04940"
     CDS             4580..>4676
                     /locus_tag="VFR20_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR1/FUN34/YaaH family transporter"
                     /protein_id="HEU0229895.1"
                     /translation="MAEESKLANPAGLGLIGFGFTTVMLSLINAGV"
BASE COUNT          903 a         1470 c         1499 g          804 t
ORIGIN      
        1 ccccctcacc gcccataggc gctgctgtcc gggttgtcca gcgtggaatg gtgccggctg
       61 tcgtgcatcg cgatgattgc gatcaacccc agggcggcga tgatcaccac ataccacgga
      121 aacacgccgc cgataccctg attcttcatg tacagcgcaa catacgaggc cgacccgccg
      181 aacagtgaat taccaatggc gtaggtgaag ccgacgccca ggccgcgcac gtgggcgggg
      241 aacatctcgg acttcacgat cccggagatc gacgtgtaga aactcagaac gaccatcgcc
      301 aacagaatga gcagacccac ggttaccgga ttctgatgcg tgccgatttc agacagcagc
      361 ggcacaggca ggatgatcat gccaagggcg aagataagca tggagttctt gcggccgatg
      421 cggtcggaga tcatgccaac caggggctgc agcagcatga acacaaacag gcagacagtc
      481 atgatctcgg cgaccaggcc tttgttcagg ccgccggtgt tgatcaggta tttctgcatg
      541 taggtcgtga aaacgtagaa cgacagcgaa ccgatgcagg tgatgcccag cacgacccag
      601 aacgcgcgcg ggtaacgcaa cacttcggcg aacgagcccg atcccctgtg tgtccggtcc
      661 ttgctggtcg tggtttcttc caggcccgag cgcagccaca acgagaccag cgcggccacg
      721 gcgccaacga caaaaggaag ccgccaccaa ccggccgtga tctgatctcc gcccagcacg
      781 tgcgtcatga tgaccgcgag caggctggcc agaagctggc cacctatgag cgtcacgtat
      841 tggaaggatg agaaaaagcc tcggcggccg cgcggcgcga tttccgacat gtacgtggcg
      901 gtggagccat attcaccgcc caccgatatg ccctggatgc atcgcatgag gatgagcagc
      961 accggcgcga agctgcccac ggtggcggcg gtcggcagga cagcgagccc aagcgaaccg
     1021 aagcccatca tgacgatgga gaccaccatg gcgcgcttgc ggccgtagcg gtccgacatg
     1081 cgcccgaaca gataaccgcc tatcggccgc atgagaaaac cgatgaagaa aacgccggcc
     1141 gcctgcatat aggcggccgt ctgccccacg ccggcgaaga actgctcggc gaaatacaca
     1201 ctgaagaaag cgtaggtgta gaaatcgaac cattcaacca ggttgccact ggacgcggcg
     1261 agaatggatt tgacgacctt gcccgtcgaa gggctgtttt ccggggtgcc aagggcctgg
     1321 ggttggccgg tgaggccggg tgcgacgaca gacatgcaag aactccttgg gctcgcctcg
     1381 ggcaccgtgc cgcgcggcgt gtctcctgat gtgtgttggg ggaaggcccc gcgtgtgtcc
     1441 ggggggcggc ttggtggccg tgatatcagc caccggatgt tgtggctgga gtatccgccg
     1501 cccacacgct ccgcacatcg gggcattcca ccccccgcgt ctctgtaaaa ctacgtagac
     1561 ggacggcgcc gtcggccgcg gcggccgtgc tatcgttgcc aacggagtgc cgatacgttc
     1621 atgacgccga ccgcccgatt tcctctatta cgccattggc gctggggcct tgccgctctg
     1681 gcctgcctgg cgctgatcct ggctgtcggc cgctggtcgc agggccagga gctgaagctc
     1741 aaggcggcca cgctgcacca ggcctccgag gcgtatgtcc tcgccctgcg cggcgtggtc
     1801 gagaaatacg acgacctgcc ctatgtggcc gccctccatc cggcgttgcg gaatctgctg
     1861 tcgcagccga ccgaccacgc gctggtcgac aaagtgaacc gctatctggc caacctgcaa
     1921 cggcgcacgg gggcggagga tctgtacgtg atcgataccc aggggctgac gctggccgcc
     1981 agcaactgga acacccaggc gagtttcgtc ggccaatcgt acagccggcg tccttatttc
     2041 gaggatgcgt tgcaggggcg gcgcggcttg ttctatggca ttggcctgac gaccggggta
     2101 cccggcctgt tcatcgccga gccggtgcgc gacgcgggcc gcgtcatcgg tgtggtgacc
     2161 gtgaagctga gcatggaatg gctcgagcag gcctggtcgc acgccgtcga tccggtcgtg
     2221 ctgcaggacg gccggggcat cgtgttcctg agttcggtgc ccgcgtggct ctaccacacc
     2281 ggccacaccc tcggaagcgg tgatctggaa tggctgcgcg agcacagcca gtacggcgca
     2341 cgggaccact acgaccgcgt gccctggcgc atcgagcgtg acgccgacat gccgggcctg
     2401 cgtctgcaaa cccgcctgga caatagaaga aaagactttc ttgcgctgca aacgaaactg
     2461 ccaaagctgg gctggaccct gaccgtgacc agcgatctcg ataccgtgcg ccgcgcgcgg
     2521 cagaaagccc aggccatcgc cacgctgatc gccgccctgc tgctgctcgg cctgctgtac
     2581 tggcgcctgc gcgaaaagcg ctacgccgag cagcggcaag cccgcatcga gctggagcgg
     2641 cgcgtcgagg aacgcacccg ggagctgcgg gaagcggagg ccttccgcaa gtcgatggaa
     2701 gactccctgc tggtcggcat gcacgcgcac gatccgcacg gccacatcat ttatgtgaac
     2761 ccggcactgt gcgagatggt cggctaccgc caggaggaac tgctgggcag cctgcctccc
     2821 tatccatact ggcatccgga tgacgtcgat caactcgcgc acgagaatga tccggcacag
     2881 ggcgatgtgg cggcgcatgg ctacgaggcc cgtttccgcc accgcgatgg ccatgaggtg
     2941 ctcgccatgg tctacaccgc gccgctgatc gatgcgcaag acgcgcatcg cggctggatg
     3001 agctccatcg tggacatcac ggagcagaag cagaccgagg cccgccagca cgagcaagaa
     3061 cgccggctac aactgagcgc gcgtctggca agcgtggggg aaatggcctc aaccttggcg
     3121 cacgagctca accagccgtt gatggccttg tccaatttcg cggtggccgc gcgcgccatg
     3181 gtcgggcatg accaacccgg tatgctggtg acggccctgg acgagatcgt tcaacaggcg
     3241 acacgggcaa gcgacatcgt caagcgtgcg cgagccttca tcaatccggt gcaggccagc
     3301 cacgaaacgc tggccatcaa cgagttggcc acgcatgcgc tggcactgct gcagcccgca
     3361 ctgcgacgcg accgcgtcac ggtgcagacc tcattggcgg cggacttgcc gctcattagc
     3421 ggagaccggg tgttgctgga gcaagtcgtg gtcaatctga tccagaacgc ggcacaggcc
     3481 atgcgggata cgcgcccccc tctgcgacgt atcgagcttt ccacgcgact ggccggatcc
     3541 gccatggaag tatcggtggc cgaccacgga ccgggcgtac ccatcgacca gatggagcaa
     3601 gtgttcatgc cgttcttcag cacacgcgcc gacggtctcg ggctgggtct caatatttgc
     3661 cgcaccatca tcgaagcgca tggcggccgc atgaccgtca tcaaccgtcc gcaaggcggc
     3721 gccgacttca ccttcactct gccgatcaaa tcatgaaaga cgcgcccctg acgctgtatg
     3781 tggtggacga cgatgaggcg ctgcgccgtt ccctgttgat gctgttgttc tctcaaggcg
     3841 tgccggtgca gtctttcgag tccggcgagg gttttctgga taccgtggat atgcgccaac
     3901 ccggctgtgt gatattggat ctgcgtatgg gcggtgtcag cggcctggag gtgctcgaac
     3961 ggttgcgtgt cgcgcacagc ccgctggtcg ccttgttttt gtccggccat ggcgacattc
     4021 ccaccgccct ggaggccatg cgcaacggcg catatgactg ggtggtgaaa ccggacaccc
     4081 agcggctcct ggagaaactt cccttggcgc tcgccgaggc gcgggcccgt ggcgccgcgt
     4141 tgcacgactg gggcgagctc acgcctcgcg aacgggaggt cgccctgctg gtcggactgg
     4201 gccagccaaa caaagagatc gcccgcatac tggttccgcc ctgcagccca cgctccgtgg
     4261 aaacccaccg ggccagcatc ttcaacaagc tgggggtcgc caacgacaac gagttgggac
     4321 gttggctggc ctcccatccc tggctcgttt gacatccgcg tgaccggtgc tcccgactga
     4381 tgcgggcaat acatacatgg cggttcgttt gggtcgcgca ggccgcgccg accgcgcgca
     4441 tcgacgcgca ccattcgcgc caggtacgtt cagctgaatg tcatgttaca gccgtgccct
     4501 tgcttgaaat gtttatccgt tgagcgcatg atgagcacac tacagagggg gggttgagtg
     4561 tcttagacag gagacttcca tggctgaaga aagcaagcta gccaaccctg ccgggttggg
     4621 gctgattgga ttcggattca ccacggtgat gttgagtttg atcaatgccg gggtgt
//
LOCUS       DASTNK010000063        23638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_970231, whole genome shotgun sequence.
ACCESSION   DASTNK010000063 DASTNK010000000
VERSION     DASTNK010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 23638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23638
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970231"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /gene="oxc"
                     /locus_tag="VFR20_04945"
     CDS             <1..1476
                     /gene="oxc"
                     /locus_tag="VFR20_04945"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HEU0229896.1"
                     /translation="ICMTVSAPGFLNGLVALANATTNCFPMILISGSSDRNIVDLEQG
                     DYEELDQMNAARRYAKAVYRVNRPQDIGIGIARAIRAAMTGRPGGVYLDLTAEVLGAA
                     IDAEAGRKSLIKVVDPAPRMIPAPESIQHAIDVLATAERPLVILGKGAAYSQADQEIR
                     SFIENTGVPYLPMSMAKGLLPDTHKQSAAAARSFAIGNADVVVVIGARLNWLLSHGRS
                     PLWSEKTKIIQIDIAATEMDSNRAIMAPVVGDIKSVMRALNAALKPGQVKPGAQWLKD
                     IGERKQKNADKMAARLKAEPSPMNYHSALRAIRDVLSERPDVYVVNDGANTLDNARNI
                     IDMQVPRHRLDSGTWGVMGIGLGYSIAAATESGKPVVAIVGDSAFGFNGMEIETVCRY
                     QLPITTIIFNNNGIYRGDPEGSPVSPTGLTPNSRYDKMIEAFGGVGHNITDTPSLTKA
                     LREAIASGKPTLINAVIDPMAGTESGHIESLNPKSALAGKK"
     gene            1816..3075
                     /locus_tag="VFR20_04950"
     CDS             1816..3075
                     /locus_tag="VFR20_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0229897.1"
                     /translation="MLRKVISYLIAGSLLLFSALAAAASAPSEIKIGTLYASSGPFAA
                     ISMPVHLGLKLWAADENAKGGVMVKPYGKRIPIKLIAYDDQSNTATAATLYNQLITQD
                     KVDILVADSGSVLTSMAVPLAREHKMFLLDQTGTGGTFFTPDNKYIALMSDPVSTIWP
                     KYIADFLTKEGPKLGIKRIALIYATNDFTGTQANAVRKMINDAHAPVKIVYDKGVPTS
                     TSNYTVLLNSIRAAQPDAVIQMGYPGNDVAFLRNLHDSGAHFKWLFAIYPGLETEHML
                     KTVGVDGMKYVYTYTPSSVVQYKVESGMSLPEFHKAWDKTYPNTKVSFGFNSVAGYTT
                     GLVIQNALAVTKSMKQLDLREAVFSLSGKLKTLDGTFELDKDGAQIGEITPLGQLVPD
                     GKGGLKFSIVYPTDVATGKPVYPAPAK"
     gene            3210..4088
                     /locus_tag="VFR20_04955"
     CDS             3210..4088
                     /locus_tag="VFR20_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0229898.1"
                     /translation="MLEYSILAGLLFGLYFSLVGLGLNLVFGVMRIVNLAHGDFVMLG
                     ALAACGLYGAYTVNPIAAAIGSLIIFIIIGFPLYYLLVPRLLKSPDPEMLSIILFFGF
                     SQVIESITTMVAGPTERSIPGNALGGSHPVTILGQTFPAAWVSSAIASAIAVALLFLY
                     LYHTRIGRLTRAVMVHREEALATGIDVGKVSGLAFGIGLGLAAVAGTFAPFMLGGISP
                     ASGVDLTIKSFTVIIVGSLGSPLGTVLGGLIYGVSLMIMQTYFSSWAGLLPYLLLIAI
                     LLVRPSGLLGRKVRHA"
     gene            4081..5001
                     /locus_tag="VFR20_04960"
     CDS             4081..5001
                     /locus_tag="VFR20_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0229899.1"
                     /translation="MPRSFRNTLLVLIPLILIAAFAPLVYKNQLLLFNFIVFLALAQG
                     VNIIYGFTGYLPFGYVGFFGAGAYGFALAVMHLHAGPMAAMAVGALVSVIFGMVLTPM
                     LRLSGPYFAIANLAAALAIAEIVSNPALESITKGPYGVSLSGIFAPTFSYGTAIAVLV
                     FATGCVLYLRYSRFGLALQAVRDDPISAAMAGINVVRARTVAWLLSALIAGLIGANFA
                     WYVSVFYPDNVFSPDFSIFAIVFALFGGVATVLGPILGVVLLYGLYNYIGISTPQYFQ
                     LIYGLLIMALVLFLPNGLASLLRRWGIHVV"
     gene            4991..5743
                     /locus_tag="VFR20_04965"
     CDS             4991..5743
                     /locus_tag="VFR20_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0229900.1"
                     /translation="MLFDPVDIPASAEPLLAVSGLVKKFGGFQALGGANFDVRPGEVL
                     GLVGPNGSGKTTCINVITGLYRPDGGSITYRGQSIGGLASHKLVRLGINRSFQIPKPF
                     KSLTVRQNIDVAVTYGGGKFGQGDIQHLMEFLELDTHADRLASELTSAQQKMLDLARA
                     LATQPKLLCVDELGAGLNPAELEQVAARLRQLANAGLALIVVEHLMDFVDQVTDRLIV
                     LSAGKNIFSGPLSQAVKDKQVVEVFLGAPDEH"
     gene            5733..6449
                     /locus_tag="VFR20_04970"
     CDS             5733..6449
                     /locus_tag="VFR20_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0229901.1"
                     /translation="MSTELLRASGLDTGYGKIQALWGVALTIHAQESVVLIGPNGAGK
                     TTLIKTLMGLIPHWQGEIHFNGKAITHERTDRRVRAGIAYMSEVGCFPDLSVEDNLLI
                     GGQFLPAAESKRHIAGLYERFPTLQQKRKALGGSLSGGQRKILGVAKALAGQPKLLVM
                     DEPSAGLSPLFVKEVIQALAQFRDTGLALLIAEQNVKFLDIADRVYTLEGGRIGFEGT
                     VEQMHKDAALERAYFGLQHH"
     gene            6526..6906
                     /locus_tag="VFR20_04975"
     CDS             6526..6906
                     /locus_tag="VFR20_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0229902.1"
                     /translation="MNHPSWIVREAEVPGYHPANHTGTLNRRLISRETVGATHVEVLL
                     GTIEKNQGALPHAHPGIEQVCYLLSGSADVEVEGERGSLEPGDCCYFPPDKKHIFTVT
                     SEEPARVLVIYSPPYEENPARVMR"
     gene            complement(6896..7981)
                     /locus_tag="VFR20_04980"
     CDS             complement(6896..7981)
                     /locus_tag="VFR20_04980"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU0229903.1"
                     /translation="MATSVTLEQRAQGALMGAFVGDALALGPHWYYDLDELRAAYGDW
                     ITDYTDPKPGRYHAGLKAGQSSQAGWLLDLTLRSLAERHAYDEADFCHRLDEDLFPFI
                     DGSPQCGPGGYTSQSIREAWRLRTQKGLPWGRVGGYADTTEAAERTLAIAVRYALQPG
                     ALARHVASNTLVTQIDPTVSAMTVAYCSILGLLVRDGVPLDERVADSLLTLVRNGELP
                     FHTVTSEGEDVPPAGLEAPRRAGEFSSPDALLSISGIARAARDPGVRIEPAWKASLVY
                     GMPCAVYHQFPAAYYLAARFQDDFESTVLHAVNGGGQNQARAMLAGALSGAIGGIQAI
                     PRRFIDGLENSAGRMALVCRVARQISA"
     gene            8320..9402
                     /locus_tag="VFR20_04985"
     CDS             8320..9402
                     /locus_tag="VFR20_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009456069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEU0229904.1"
                     /translation="MSATPTALSPDHAHGSAKHHKKHRSGNASQSQKPNVLLGLVLAA
                     AVGAVALVAGRWMPLIGGPVFGIIFGIIIRNAIGVGPLFRPGLQFASKQVLQWSIIAL
                     GFSLSIQQVAKTGLESLSVTFVTITVAFVSAYLLGKWLNIPSKLCTLIGVGTAICGGS
                     AIAAVTPIIKADDHDTAFAISTIFLFNIVAVLTFPLLGHWLHMTDMGFGMWAGTAIND
                     TSSVVAAGFSFSHAAGDFATIVKLTRATLIIPVCLGLAFYEAWRHKKHGSGDFSLARI
                     FPWFILWFLVASIIRSTGLLPESWNSGLHFLAEFLIIMALTAIGLSSDLRRMASAGIR
                     PILLGLGVWAAVSLSSLMVQHYMGSW"
     gene            9543..10781
                     /locus_tag="VFR20_04990"
     CDS             9543..10781
                     /locus_tag="VFR20_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0229905.1"
                     /translation="MDHGGAVVDRRHFLRMGLGLGAGAALGGLAGRASAAESSATIDL
                     PFANGLRQMATFPQKRPLILLTSRPPQLETPFKVFNEGIFTPNDAFFVRYHWSGIPTS
                     IDPRTYRVRIKGLVDTPLSLSLDELKKMGTVTLAAAHQCSGNSRGFANPRVNGGESGN
                     GAMGNARWTGVPLKKVLEKAGLKAGAVQVSFNGLDHPPLGDGPDFIKALDIDHAMDGE
                     VMLAWSMNDADLPMLNGYPLRLIVPGYYGTYWVKHLSDITVMDKVLANFWMAKAYRVP
                     DDACACVPAGGKMVHSRPIGRFAIRSFLTNVLDGDTIPAGKATLLRGFAFDGGYGLTA
                     VNVSADGGKTWSRAKLGEDHGKYSFREWTLPFTPAKAGPLEIKVRAFNKIGQTQPMEA
                     LWNPSGYMRNVVETTRVIAA"
     gene            10793..11491
                     /locus_tag="VFR20_04995"
     CDS             10793..11491
                     /locus_tag="VFR20_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU0229906.1"
                     /translation="MLKKRNLTTLAGTAALLAGLCTSASALEITLPPETATLKPSTLP
                     GYQKALQNCTACHSAQYMQTQPASSHAWWTAEVHKMKNAYGAPIAEADMNAIADYMFA
                     VYGPGKGADEANAPGAKPALAVATTADATTRTARADGKTLKLDAQALLKANNCLACHA
                     VDHKVVGPAYNDVAARYAGKPDAEATIARHIREGGSGKWGPVPMPPFSNLTDAQSLVL
                     ASWIVGLGADKHSK"
     gene            complement(11590..12363)
                     /gene="pstB"
                     /locus_tag="VFR20_05000"
     CDS             complement(11590..12363)
                     /gene="pstB"
                     /locus_tag="VFR20_05000"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEU0229907.1"
                     /translation="MPTLLDESIKIDVRNLNFHYGKFHAIRDVSLPIREKKVTAFIGP
                     SGCGKSTLLRTFNRMFELYPGQRATGEINIDGENLLTSRTDISLIRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSKGEMDERVEWALYKAALWGEVKDKLRQSGNSLSGGQQQ
                     RLCIARGVAIKPEVLLLDEPCSALDPISTAKIEELIAELKADYTVVIVTHNMQQAARC
                     SDHTAYMYMGELMEYGPTDQIFLKPARKETEDYITGRFG"
     gene            complement(12479..13342)
                     /gene="pstA"
                     /locus_tag="VFR20_05005"
     CDS             complement(12479..13342)
                     /gene="pstA"
                     /locus_tag="VFR20_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU0229908.1"
                     /translation="MNPRDSGAGRPNGVYRRRRFVNRVMLTLSMLALLFGLFWLCWIL
                     LTLLYKGGAAMSWTLFTETTPPPGADGGLLNAIVGSVMMTAVGTLIGTPVGILAGTYL
                     AEYGRRGWLAPATRFLNDVLLSAPSIIIGLFIYAVYVAQVGHYSGWAGAFALSILVIP
                     VVVRSTDNMLQLVPNSLREAAAALGCPQWRIVVFVCYRAAWSGIVTGVLLAVARISGE
                     TAPLLFTALNNQFMSWNMNAPIANLPVIIFQYAASPFSDWNRLAWAGAALITLIVLGI
                     SITARVLFRKK"
     gene            complement(13356..14291)
                     /gene="pstC"
                     /locus_tag="VFR20_05010"
     CDS             complement(13356..14291)
                     /gene="pstC"
                     /locus_tag="VFR20_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU0229909.1"
                     /translation="MNTSRSGVSDVLFRNVTRAFAFLVFALLAAIMVALVYGSRDSIV
                     KNGLAFLWTNDWNPVRHHYGALVPVVGTLLTSFLALLIAVPVSFGIAMFLTELSPRWL
                     RGPLGTAVELLAAIPSIIYGMWGLFVFVPLFQQYVQPGLIWLFHDVPVLRDVFSGPPF
                     GIGVFTAGLILSIMVIPFITAVMRDVFEVVPPMLKESAYGLGGTTWEVMWKVVLPYTK
                     NGVIGGIMLGLGRALGETMAVTFVIGNAFTLPSSLFSPSNSIASALANEFNEAGGLQK
                     SALLEMGLILFLITTIVLALSKVLLSRLGGSEGKR"
     gene            complement(14309..15352)
                     /gene="pstS"
                     /locus_tag="VFR20_05015"
     CDS             complement(14309..15352)
                     /gene="pstS"
                     /locus_tag="VFR20_05015"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU0229910.1"
                     /translation="MNLKVVLKVVAGVAASSVVLAAQAADITGAGASFPYPVYAKWAT
                     LYNKATGNRVNYQSIGSGGGQQQIIAKTVDFGASDDPMKGPDLEKNGLLQFPAVIGGT
                     VPVVNVQGIAPGQLRLSGPVLADIFLGKVKMWNDPAIAALNPGIKLPGAAIIVVHRSD
                     GSGTTFGWTNYLSKVSPAWKSQVGEGKAVKWPVGQGGKGNEGVAAYVRQLHNAIGYVE
                     YAYAKQNHLSWVGLKNRDGQFVQPTQGAFAAAAENADWNSAPGMGVVLTDEPGARSWP
                     VTSATFILVHKKQENPEKARQVLAFFDWAFKNGAKAAEALDYVPLPAEVTSKITQAWK
                     TQIKAADGSAVWK"
     gene            complement(15527..17635)
                     /gene="ppk1"
                     /locus_tag="VFR20_05020"
     CDS             complement(15527..17635)
                     /gene="ppk1"
                     /locus_tag="VFR20_05020"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEU0229911.1"
                     /translation="MVSPLPEPALQNRELSLIKFNERVLAMAESRATPLLERLRYLCI
                     VGSNLDEFFEIRIASLKEQQRRPVGVLGGDALTPDQAFEQVQLAAHRLVDRQNSLLMN
                     DVLPELLTHGVGLLVSTTWTEAQWAWARETFDRDVMPLLTPIGLDPAHPFPRVYNKSL
                     NFIVSLSGQDAFGRRASIAIVQAPRALPRVIRMPPEVAGISDGYILLTTLISAFVGEL
                     FPGLDVKGCYQWRVTRNSDLYVDEEEITNLRQALQGELSQRNFGASVRLEIDHGMPPS
                     LESFLQREFVLEPQDTYRVSGPVNLARLLQMCDLVDRPELSFPAFEPAIPSPFDTLGG
                     DPAGLFRAIAQRDHLLHHPYQSFQPVIDFLTAAAHDPDVVAIKQTIYRTGEDSELMRL
                     LLLAARAGKEVTVVVELMARFDEQTNINWAAKLEAVGAHVSYGVVAHKTHAKMALLLR
                     REQGRIRRYGHLSTGNYHPRTAKLYTDFGLLTANPAICEDMDKVFSLLTGLGARRPLK
                     LLLQSPFTLHETMVALIKAEAAHARAGRKARIMAKMNSLLEPIIVEELYKAGQAGVKI
                     DLIVRGACALRPGVRGLSDNIHVTSIIGRFLEHARVFYFYDDGAENVYLASADWMDRN
                     FFRRVEVGFPVLDKTLKKRVINEALTSALRDNQLAWRCQADGAYARVHNRRPAFNLHD
                     SLMRSLGSRVGPDARTEPEC"
     gene            complement(17758..18021)
                     /locus_tag="VFR20_05025"
     CDS             complement(17758..18021)
                     /locus_tag="VFR20_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEU0229912.1"
                     /translation="MYVVELTYLKPLDAIEVHLEARHRLYPEMRCHPAGYFDRVARCG
                     TASLNKLATADAVVYATARHLRAELLTCDAHFEKLPGVALHAK"
     gene            complement(18088..18759)
                     /locus_tag="VFR20_05030"
     CDS             complement(18088..18759)
                     /locus_tag="VFR20_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU0229913.1"
                     /translation="MQTKKANDMSDPASHSQNDSFDALVRAIRACRVCRDEPRYGARL
                     DHEPRPIIQVSRTARICIASQAPGIRVHESGRPFDDRSGIRLRQWMAIDESVFYDESR
                     IAILPMGFCFPGNDANGGDLPPRRECAEIWRSKLFAHLPDLKLVLVIGGYAQRWHLGA
                     ACARQGVTQTVQAWRELYRANPAPHVFPLPHPSWHNHNWLKRNPWFETDMLPVLREDI
                     RAALA"
     gene            complement(18814..19023)
                     /locus_tag="VFR20_05035"
     CDS             complement(18814..19023)
                     /locus_tag="VFR20_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2945 domain-containing protein"
                     /protein_id="HEU0229914.1"
                     /translation="MTERFRVGDHVGWNSEAGRVSGTIIAIHTQDFDYKGHVHHASED
                     DPQYEIKSDKTDHIAAHKGRVLTRI"
     gene            complement(19020..19406)
                     /locus_tag="VFR20_05040"
     CDS             complement(19020..19406)
                     /locus_tag="VFR20_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEU0229915.1"
                     /translation="MSRSSVPTSHVRVKRAYEPASPDDGVRILIDRLWPRGVSKDKAA
                     LDDWMKDLAPSTELRQWFGHDPARWDEFQQRYGAELDQNPEALDRVRTLAKTQTVTLV
                     YSAHDEAHCNAVVLRNVLLDGKAHGK"
     gene            complement(19571..20704)
                     /locus_tag="VFR20_05045"
     CDS             complement(19571..20704)
                     /locus_tag="VFR20_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEU0229916.1"
                     /translation="MTGIHDLNTPAALVDVQRMRRNIERMQARMNALGVRFRPHVKTT
                     KCLPVVQAQIVAGAQGITVSTLKEAEQFFASGVSDILYAVGMAPDKLPRALALRRRGC
                     DLKLITDNVEAAREIGRFGKLNDEEFEVWVEIDTDGHRSGIKPEEDSLLDVGRALHEA
                     GMRLGGVMTHAGSSYDCDTDAALRAMAEQERAGAVRAAQRLRGAGLPCPVVSVGSTPT
                     ALSAEHLEGVTEVRAGVYVFFDLVMRNVGVCAMDDIALSVLATVIGHQGEKGWAIVDA
                     GWMAMSRDRGTQKQKQDFGFGQVCAEDGAVLEGYVLSGANQEHGILSSTGAPDPHIAR
                     RFPVGTRLRILPNHACATGAQYPEYQAIYPDGRVEAWPRFYGW"
     gene            complement(20770..21381)
                     /locus_tag="VFR20_05050"
     CDS             complement(20770..21381)
                     /locus_tag="VFR20_05050"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEU0229917.1"
                     /translation="MNDHDKIFQSLDKRGWAVSDTVIPLSWHITLLMLGWQLWQDGCF
                     HMEGIGRGVSDARRPDIRGDTVCWVPPGSLLSRHPFFSWMARFRQELSERTNLGLRSQ
                     EFHFARYGKGTGYRKHIDQHRDTDHRKISIVLYLNLQWNPMYGGDLCLYEPEAPNVEM
                     RRISPQGARLVVFHSGLVPHAVLPCQQTRWSLTGWLRSDEIAG"
     gene            complement(21539..22063)
                     /locus_tag="VFR20_05055"
     CDS             complement(21539..22063)
                     /locus_tag="VFR20_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU0229918.1"
                     /translation="MEYTFTLKYLLSSSVYDLDALVESLGEAGCDDALVGIGQPGRLA
                     LAFTREAACAEDAVHSALADVRRAVPSASLIEAAPDLVGLTDVAEATGMSRQNMRKLM
                     LTHPDTFPAPVHEGSASIWHLVDVLGWLQAQGGYTLDGALMDVAETTLRVNVAKEGRR
                     LWNKPSAEIDALVG"
     gene            complement(22068..22343)
                     /locus_tag="VFR20_05060"
     CDS             complement(22068..22343)
                     /locus_tag="VFR20_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229919.1"
                     /translation="MSRPLHPKKEIEEALRYIERQGWRVEVGDSHAWGRVYCPYDDEA
                     CRCGEFCIVSVWSTPKNPGNHARALRRVVDNCIRHRKLRETEGGAEE"
     gene            22676..>23638
                     /locus_tag="VFR20_05065"
     CDS             22676..>23638
                     /locus_tag="VFR20_05065"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEU0229920.1"
                     /translation="MNTTDSAAISAVNRFGLGGKPGELEGVKQPREWLLSQVARGPDG
                     AALFVGLPSSLDYLGEEARLQQTRHELKQKERSRRDFLTGRPSTEDAADGAAALKQAL
                     KPYREEERKDVLAEAAARYQVAATTDTPFVERLVHFWSNHFAVSTDKGPVRLLAAPME
                     REAIRPHVTGRFADMLLAVETHPAMLLYLDNIRSVGPNSPLGRRIAKRLARKGARDEG
                     KAGGLNENLGREAMELHTVGVNAGYTQADVTEFSRALTGWSIPFPRDLVGDYPLQSAF
                     MFRANAHEPGARRVMEHTFPEGGFEQGRAILEFLAHQPATALHLS"
BASE COUNT         4607 a         7700 c         6964 g         4367 t
ORIGIN      
        1 atctgcatga ccgtttcagc acccggtttc ctgaatggcc tggtcgcgct tgcgaacgcc
       61 acgaccaact gcttccccat gattctcatc agcgggtcca gcgaccggaa cattgtcgac
      121 cttgaacaag gcgactatga agagctcgac cagatgaatg ccgccaggcg ctatgccaag
      181 gcggtctatc gcgtcaatcg tccccaggac atcggcatcg gtatcgcgcg cgcgattcgc
      241 gccgcgatga cgggacgccc gggcggggtc tacctcgact tgacggccga agtgctgggc
      301 gcggccatcg acgcggaagc cggccgaaaa tcactgatca aggtcgtcga tccggcgccg
      361 cgtatgatcc cggcaccgga atcgatccaa cacgcgatcg acgtgctggc caccgccgaa
      421 cgtccgctcg tcattctcgg caaaggcgcg gcctactcgc aggccgacca ggaaatccgg
      481 tcgttcatcg aaaacaccgg tgtgccctac ctgcccatgt ccatggcgaa gggcctgctg
      541 cccgacaccc acaaacagtc ggcggcggcg gcgcggtcgt tcgccatcgg caacgccgat
      601 gtggtggtcg tgatcggtgc gcgcctgaat tggctgctgt cgcacggccg ctcgccactg
      661 tggtcggaaa agacgaagat catccagatc gacatcgcgg ccacggaaat ggacagcaac
      721 cgtgccatca tggcgcccgt cgtcggcgac atcaagtcgg tgatgcgggc gctgaacgcc
      781 gcgctcaagc cggggcaggt caagcccggc gcgcaatggc tcaaggacat cggcgagcgc
      841 aagcagaaga acgccgacaa gatggctgcg cgcctgaagg ccgaacccag ccccatgaac
      901 taccacagcg ccttgcgcgc gatccgggat gtcctgtcgg agcgtccgga tgtgtatgtg
      961 gtgaacgacg gggccaacac gctggacaac gcacgcaaca tcatcgatat gcaggttccc
     1021 cgccatcgcc tggacagcgg tacgtggggg gtgatgggca tcggtctggg ttattccatc
     1081 gccgcggcga ccgaatccgg caaacccgtc gtggccatcg tgggcgacag cgcgttcggg
     1141 ttcaacggca tggaaataga aaccgtctgc cgctaccagc ttccgatcac caccatcatc
     1201 ttcaacaaca atggcattta tcgcggcgac ccggaaggca gccccgtctc gccgacgggt
     1261 ctgacaccca attcgcgcta cgacaagatg atcgaggcct tcggtggcgt ggggcacaac
     1321 atcaccgaca cccccagcct gaccaaggca ttgcgggaag cgatcgcgtc gggcaagccc
     1381 acactcatca atgccgtcat cgacccgatg gccggcaccg agagcggcca tatcgagagc
     1441 ctcaacccca agagcgccct ggctggcaag aagtaagtcc gcgggtgcgg ttatagcgac
     1501 tcggcaagga gtcctgtagc cgaacccggc cggcgacagc cctttttgca gggccatcgc
     1561 cggcctcggt caagcacggt atccacatcg ggccttcggg cccggttttc tttgtccgcg
     1621 ctcgtcgtca cgatgatggc ggatccatga cctccatcac tcgccggttt cgcttttctt
     1681 cgtatttctt cgatcgaaaa tccgggaaaa ccatggttac ggaattcaga ccggcgtaag
     1741 agtcgcaggt ttaacatggt cggcgatagg ccatgctctg cgggccttcg ctcaaaacaa
     1801 tcggagaccg aacccatgtt gcgaaaagtc atttcctacc tgatcgcagg ttcgctactc
     1861 ttgttttccg ccttggccgc cgctgcgtcg gcgccctcgg aaatcaagat tggcaccctg
     1921 tatgcgtcat cggggccgtt cgcggccatt tccatgcctg tgcatctggg gctcaagctc
     1981 tgggccgccg atgaaaacgc caagggcggc gtcatggtca agccctacgg caagcgtatc
     2041 ccgatcaagc tcatcgccta cgatgaccag agcaacaccg ccaccgccgc cacgctgtac
     2101 aaccagctca tcacgcaaga caaggttgac atcctggtcg ccgacagcgg ctcggtgctg
     2161 acgtccatgg ccgttcccct ggcgcgtgaa cacaagatgt tcctgcttga ccagaccggc
     2221 accggcggca cgttcttcac gcccgacaac aagtacatcg ccctgatgtc cgaccccgtg
     2281 tccaccatct ggcccaagta catcgccgac ttcctcacca aggaaggccc gaaactgggt
     2341 atcaagcgca tcgccctgat ctacgcgacc aacgacttca cgggcaccca ggccaacgct
     2401 gtgcgcaaga tgatcaacga cgcgcatgcg ccggtcaaga tcgtgtacga caagggtgtg
     2461 cccaccagca cttcgaacta caccgttctg ctcaacagca tccgcgccgc gcaacccgat
     2521 gccgtgatcc agatgggcta ccctggcaac gacgtggcgt tcctgcgcaa cctgcatgac
     2581 tcgggcgcgc acttcaagtg gctgttcgcc atctaccccg ggctcgaaac cgagcacatg
     2641 ctcaagacgg ttggcgtgga cggcatgaag tacgtctaca cctacacgcc ttcgtccgtg
     2701 gtgcagtaca aagtcgaatc cggcatgtcg ctgcctgaat tccacaaggc ctgggacaag
     2761 acctatccaa acaccaaggt gtcgttcggc ttcaattccg tggcgggcta caccacgggc
     2821 ctggtcatcc agaacgccct ggcagtcacc aagagcatga agcagctcga cctgcgcgaa
     2881 gccgtgttca gcctgtcggg caagctcaag acccttgacg gcactttcga gctcgacaag
     2941 gacggcgccc agatcgggga aatcacgcca ctgggccaac tggttccgga cggcaagggt
     3001 gggttgaaat tctcgatcgt gtacccgacg gatgtggcca ctggtaaacc ggtgtacccc
     3061 gcccccgcca aataatcact attaaaatca gccagtctta gttggttcag tacatcaagc
     3121 gcccgccatg ccgccctggg gagggaacat ggcgggccga tgtctctgca acaccatcgc
     3181 attgccactc atagcgagcc cttcgcgata tgcttgaata ttccatcctg gccggactgc
     3241 tgttcggcct gtacttcagt ctggtgggcc tgggactgaa cctggtcttc ggtgtcatgc
     3301 gcatcgtcaa cctggcccac ggcgatttcg tcatgctggg cgcgctggcg gcctgcggcc
     3361 tgtacggcgc ctataccgtc aatcccatcg cggcggccat cggcagcctg atcatcttca
     3421 tcatcatcgg cttcccgctg tactacctgc tggtgccgcg cctgctgaaa tcgcccgatc
     3481 cggagatgct gtccatcatc ttgttcttcg gattttcaca ggtcattgaa tccatcacca
     3541 ccatggtggc cggccccaca gaacggtcga tccccggcaa tgcgctgggc ggttcgcacc
     3601 cggtcaccat cctgggccag acgttccccg ccgcgtgggt cagcagcgcc atagccagcg
     3661 ccatcgcggt cgcgctgctg ttcctgtacc tgtaccacac ccgcatcgga cgcctcacgc
     3721 gcgccgtgat ggtccatcgc gaagaagccc tggccaccgg catcgatgtg ggcaaggtgt
     3781 cgggcctggc cttcggcatc ggcctggggc tggcggcggt ggcgggcacg ttcgcaccgt
     3841 tcatgctcgg cggcatttca ccggccagcg gcgtggacct caccatcaaa tccttcaccg
     3901 tcatcatcgt cggctcgctg ggcagcccgc taggcacggt cctgggcggg ctgatctacg
     3961 gcgtttccct catgatcatg cagacgtact tcagttcctg ggcggggctg ctgccctacc
     4021 tgctgctgat cgccattctt ctcgttcgcc ccagtggtct gctgggacgc aaggtgcgcc
     4081 atgcctagat cttttcgcaa tacgctgctg gtgctgattc cgctgatcct gatcgcggcc
     4141 ttcgcaccgt tggtctacaa gaaccagttg ttgctgttca acttcatcgt cttcctggcc
     4201 ctggcgcagg gcgtgaacat catttacggc ttcaccgggt acctgccctt cgggtatgtg
     4261 ggctttttcg gggcgggcgc ctacggcttc gccctggccg tcatgcatct gcatgccggt
     4321 cccatggccg ccatggcggt gggcgcgttg gtttcggtca tcttcggtat ggtgctcacg
     4381 cccatgctcc gtttgtccgg cccgtacttc gccatcgcca acctggcggc ggcgctggcc
     4441 atcgctgaaa tcgtctccaa ccccgcgctg gaatccatca ccaaggggcc gtatggcgta
     4501 tcgctgtcgg gcatcttcgc gccgacattc agctacggca cggccatcgc cgtgctggtg
     4561 ttcgcgacgg ggtgcgtgct gtacctgcgc tattcgcgtt tcggtctggc attgcaggct
     4621 gtgcgcgacg atcccatcag cgcggccatg gcaggcatca acgtggtgcg cgcgcgcacc
     4681 gtggcctggc tgctctcggc gctcatcgcc ggcctcattg gcgcgaactt cgcctggtac
     4741 gtgtccgtgt tctatcccga taacgtgttc agccccgact tcagcatctt cgccatcgtc
     4801 tttgccttgt tcggcggcgt ggccacggtg ctgggtccga tcctgggcgt ggtgctgctc
     4861 tatgggctgt acaactacat cgggatctcc acccctcaat atttccaact gatatacggc
     4921 cttctcatca tggcattggt gctattcctt cccaacggcc tggcttcact cttgcgacgc
     4981 tgggggatcc atgttgtttg atcccgtcga catccccgca agcgccgagc cgctgctggc
     5041 cgtttccggg ctcgtgaaaa aattcggcgg tttccaggcc ctgggcgggg ccaacttcga
     5101 tgtccgcccg ggtgaagtcc tgggtctggt cgggcccaat ggctcgggca agaccacatg
     5161 catcaacgtc atcaccgggc tatatcggcc cgacggcggg tccatcacgt accggggcca
     5221 atccatcggc gggctggctt cccacaagct cgtgcgcctg ggtatcaacc gcagtttcca
     5281 gatccccaag ccgttcaagt cgctcacggt gcggcagaac atcgacgtgg ccgtgacata
     5341 cggcggcggc aagttcggcc aaggcgacat tcagcacctc atggagttcc tggaactgga
     5401 cacgcacgcc gaccggctgg ccagcgaact caccagcgcg cagcagaaaa tgctggacct
     5461 ggcccgagcc ctggccacac aacccaagct gttgtgtgtg gacgaactgg gtgcgggcct
     5521 gaaccccgcc gaacttgaac aggtggcggc acggctgcgg caactggcca atgccggact
     5581 ggcgctgatc gtcgtcgagc acctcatgga tttcgtcgat caggtcaccg atcgcctgat
     5641 cgtcctcagt gccggcaaga atattttctc ggggccgctg tcgcaggccg tcaaggacaa
     5701 gcaggtcgtg gaagtatttc ttggagcccc cgatgagcac tgaactgttg cgcgccagtg
     5761 gcctggacac cggctacggc aagatccagg cgttgtgggg cgtggcgttg accatccatg
     5821 cccaggaatc cgtcgtgctg atcgggccca acggcgcggg caaaaccacg ctgatcaaaa
     5881 ccttgatggg ccttatcccg cactggcagg gcgagatcca tttcaatggc aaagccatca
     5941 cccacgagcg caccgaccgg cgtgtgcggg ccggcatcgc gtacatgtcg gaagtcggct
     6001 gctttcccga tctcagtgtc gaagacaatc tgctcatcgg cggacagttt ctgccggctg
     6061 ccgaatccaa acgccacatt gccggcctgt acgaacgctt tcccacgctc cagcagaaac
     6121 gcaaagccct gggcggcagc ctgtcgggtg ggcagcgcaa gattctggga gtggccaagg
     6181 cgctggcggg ccagcccaag ctgctggtca tggacgagcc ctcggccggc ctgtcgccat
     6241 tgttcgtcaa ggaagtgatc caggccctgg cccagttccg cgacacgggc ctggcattgc
     6301 tcatcgccga gcagaacgtg aagttcctgg atatcgccga ccgtgtctac acgctggaag
     6361 gcgggcgcat cggcttcgag ggcacggtgg agcagatgca caaggacgcg gcgctcgagc
     6421 gtgcctactt cgggctgcaa catcactgag gccgcttgcg cgccggggtg cgagcagcct
     6481 tcgcgcgttc cgccccggac atacaaacac tggcaaggta ccaccatgaa ccatccgagc
     6541 tggatcgtca gggaagccga agttcccggc tatcatcccg ccaaccacac cggcacattg
     6601 aaccgacgcc tcatcagccg cgaaaccgtg ggcgccacgc acgtcgaggt cttgctgggc
     6661 accattgaaa aaaaccaggg cgccctgccc catgcccacc ccggcatcga gcaggtgtgc
     6721 tacctgctga gcggctcggc cgatgtcgaa gtggaaggcg aacgcggcag tctggagccg
     6781 ggcgactgct gctatttccc ccccgacaag aagcacattt tcaccgtcac cagcgaagaa
     6841 ccggcgcggg tgctggtgat ctattccccg ccatacgaag aaaatccggc ccgggtcatg
     6901 cgctgatctg ccgcgccacc cgacacacca gcgccatgcg cccggcgcta ttttccagac
     6961 catcgatgaa gcgccggggg atcgcctgaa tgccgccgat ggccccggac agtgcccccg
     7021 ccagcatggc ccgcgcctga ttctggcctc cgccattgac cgcgtgcagc acggtggact
     7081 cgaaatcgtc ctggaaacgc gccgccagat aatatgcggc cgggaactgg tggtacaccg
     7141 cgcaaggcat gccgtagacc agggatgctt tccaggccgg ttcgatgcgc acgcccggat
     7201 cccgggccgc gcgcgcaatg ccggagattg aaagcagtgc gtcgggcgac gagaactcgc
     7261 cggcacgacg cggtgcctcg aggccggccg ggggcacatc ctcgccttcg gatgtcacgg
     7321 tgtgaaacgg cagctcgcca ttgcgcacca gcgtcagcag cgagtccgcc acccgctcgt
     7381 cgagcggtac gccgtcgcgc acgagtaaac ccagaatcga gcaatacgcc accgtcatgg
     7441 ccgacacggt gggatcgatc tgagtaacga gcgtgttgga cgccacgtgg cgagccagcg
     7501 cgccaggctg caaggcgtag cgcacagcga tggccagtgt ccgctcggcg gcctcggtgg
     7561 tgtcggcata cccgccaacg cggccccaag ggaggccctt ctgtgtgcgc aggcgccacg
     7621 cttcgcggat cgattggctc gtatagccgc ccggtccgca ctgtggtgag ccgtcgatga
     7681 agggaaacaa gtcctcatcc agacggtgac agaaatcggc ttcgtcgtag gcatggcgct
     7741 cggccagcga acgcaacgtc aggtcaagca gccagccagc ctgcgacgat tggcccgcct
     7801 tgagcccggc gtggtagcgc cccggtttgg gatcggtgta atcagtgatc cagtcaccgt
     7861 aggccgcgcg caattcatca agatcgtaat accagtgagg gcccagggcc agcgcatcac
     7921 cgacgaacgc ccccatcaag gcgccctggg cgcgttgttc caacgtgacg gatgtggcca
     7981 tgatgttgtt cctcaaatag acagcggtgg tgctccgccc ggcccggctc cgctcggctc
     8041 ggagcgtggg gtttcgccgt cgcgcgacca cccaccggca aagttcgaaa aaatggctgg
     8101 cagcctcatc gcccgacgcc aaccacaagg tacccccacc tcggggttct ggcaaataca
     8161 tgccgttcac tggcgagaac gcgaaccgcg agacacctgc cccatcttca accctatcaa
     8221 taagttttat ataaaaaata aatataaatg aatttattta tataaccgca cgccctagaa
     8281 ttcatgacat ccatctattt gtttattcga gaccccgcca tgtccgccac gcccacagcc
     8341 ttgtcccccg atcatgccca tggctcggca aagcaccata aaaagcaccg ttcggggaac
     8401 gcctcgcagt cccagaaacc caacgtcctg ctcggcctgg tgctcgccgc cgctgttggc
     8461 gcggtggccc tggtcgccgg gcgctggatg ccattaatcg gcggaccggt gttcggcatt
     8521 attttcggta tcatcatccg caacgccatc ggtgtcggcc ccctgttccg accagggctg
     8581 caattcgcct ccaagcaagt gttgcagtgg tccatcatcg cactgggttt cagtctcagc
     8641 atccagcaag tcgccaagac tggtctggaa tcattgtcgg tgacgttcgt caccatcacg
     8701 gtggccttcg tatccgccta cctgctgggc aagtggctca acattccgag caaactttgc
     8761 acgttgattg gcgtgggcac ggccatttgc ggcggctcgg ccatcgcagc cgtcacgccc
     8821 atcatcaagg cggatgacca cgacaccgcg ttcgccatct cgacgatatt cctgttcaac
     8881 atcgtggcgg tcctgacctt cccactgctg ggccattggc tgcacatgac ggacatgggc
     8941 ttcgggatgt gggccggcac agccatcaat gacacgtcat cggtcgtcgc ggcgggcttc
     9001 agcttcagcc atgcggcggg tgacttcgcc accatcgtca agctcacacg ggccacgctg
     9061 atcatcccgg tctgtctggg actggccttc tacgaagcct ggcgccacaa aaaacatggc
     9121 agcggcgact tcagcctggc gcgcatcttc ccctggttca ttctgtggtt cctggtggcg
     9181 tcgatcattc gctccaccgg cctcctgccg gaaagctgga acagtgggct gcacttcctg
     9241 gccgagttcc tgatcatcat ggccctgacc gccatcggtc tgtcgtccga cctgcggcgc
     9301 atggcctcgg ccggtatccg ccccattctg ctgggtctgg gagtctgggc agcggtatcg
     9361 ttgagcagcc tgatggtcca gcactatatg gggtcttggt aaggcgctaa acaccaaaca
     9421 taaccgaaca cacacatccc cggtggttgc tccagtcgac gcggccaccg gggatgtgtc
     9481 gctgtgaagc cgcaaaaaca caaccattac aacctgaagg ggaatgcatg aagaaaccac
     9541 acatggatca tggtggggcc gtcgtcgacc gacgccactt tttacgaatg ggtctcgggc
     9601 tgggtgccgg cgccgcgctg ggtggccttg cggggcgtgc ctcggcggct gaatcatccg
     9661 ccaccatcga tcttccattc gccaacggtc tgcgtcagat ggcgacattt ccacagaagc
     9721 gcccactgat tctcctgacc agccggccgc cgcaactcga aacgccgttc aaggttttca
     9781 acgaaggcat attcacccca aacgacgcgt tcttcgtgcg ctatcactgg agcggcattc
     9841 caaccagcat cgacccgcgc acctatcgtg tgcggatcaa aggcctggtc gacacgcccc
     9901 tgtcgctatc actcgacgaa ctcaagaaaa tgggcacggt cacgctcgcg gccgcgcatc
     9961 aatgctccgg caacagccgc ggcttcgcca acccccgtgt caatggtggc gaatcgggca
    10021 atggcgccat gggcaacgcg cgctggaccg gcgtgccact gaagaaagtg ctggaaaaag
    10081 ctggactgaa ggccggcgcc gtgcaggttt cgttcaatgg cctggaccac cctccgctgg
    10141 gcgacggccc cgacttcatc aaggctctgg acattgatca cgccatggac ggcgaagtga
    10201 tgttggcctg gtccatgaac gatgccgatc tgcccatgct caacggctat ccgctgcgat
    10261 tgatcgtgcc cggctactac ggcacctact gggtgaaaca cttgtccgac atcactgtca
    10321 tggacaaagt gctggcgaac ttctggatgg caaaagccta tcgtgtgccg gatgatgcct
    10381 gcgcctgcgt cccggcggga gggaaaatgg tccattcacg ccccattggc cgtttcgcca
    10441 tccggtcatt cctcaccaat gtgctggacg gcgacactat cccggcgggc aaggccacgc
    10501 tgttgcgcgg cttcgccttc gacggcggct acgggctcac ggcggtcaac gtgtcggccg
    10561 acggcgggaa aacctggtca cgcgccaagc tgggagaaga ccacggcaag tattctttcc
    10621 gcgaatggac cttgccgttc acgcccgcaa aggccggtcc gctggaaatc aaggtgcgag
    10681 ccttcaataa gataggccag acccagccca tggaggcgct ctggaacccg tcgggctaca
    10741 tgcgcaatgt cgtcgaaacc acacgtgtca tcgcggccta ggaatcatca tcatgctgaa
    10801 aaaaaggaat ctcaccacgc tcgccggcac cgccgccctg ctcgcgggtt tgtgcacatc
    10861 cgccagcgcg ctggaaatca cactgccgcc cgaaaccgcc acgctcaagc ccagcacact
    10921 gccgggctac cagaaggcgc tgcaaaactg cacggcctgt cactcggcgc aatacatgca
    10981 gactcagccg gcctcctcgc acgcctggtg gacggccgag gtgcataaaa tgaagaacgc
    11041 ctacggcgcc cccatcgccg aggccgacat gaacgccatc gccgactaca tgttcgcggt
    11101 atacgggcca ggcaagggtg cggatgaagc caacgcaccc ggcgccaagc cggcattggc
    11161 tgtcgcgacg accgccgatg ccacgacgcg cacagccagg gcggacggga agacgctcaa
    11221 gctcgatgcg caggcgctgc tcaaggccaa caactgtctg gcctgccacg ccgtggatca
    11281 caaagtggtc gggcccgcct acaacgatgt ggcggcccgc tacgccggta agcccgacgc
    11341 cgaggccacc atcgcccggc atatccgcga agggggatcc ggaaaatggg ggccggtacc
    11401 catgccgccg ttttccaatc tcaccgacgc ccagtcccta gtgctggcgt cctggatagt
    11461 gggcctgggc gcggacaagc actcgaaata gcgtgccggg cgccgtctgg gttcagaacc
    11521 tgttctccaa tgccccacgc cccagtcggc ggcttcattt ggggtaaaca aggtccgggc
    11581 ccgccggcgt tacccgaacc ggcccgtgat gtagtcttct gtttccttgc gggccggctt
    11641 gaggaagatc tgatcggtgg ggccgtactc catcaactca cccatgtaca tgtaggccgt
    11701 gtggtcggag caacgcgcgg cctgctgcat gttgtgcgtg acgatgacca cggtgtagtc
    11761 ggccttcaat tccgcgatca attcctcgat cttggccgtc gaaatcgggt ccagcgcgga
    11821 gcagggttca tccagcagca gcacctcggg cttgatggcc acaccgcgcg caatgcacag
    11881 acgctgttgc tgccctccag acaagctgtt gccgctctga cgcagtttgt ccttcacctc
    11941 gccccacaga gccgccttgt acagcgccca ttcgacgcgc tcgtccatct cacccttgct
    12001 gagcttctcg aatagacgaa cgccgaatgc gatgttgtcg tagatgctca tggggaatgg
    12061 cgtcggcttc tgaaacacca tgcccacctt ggcgcgtatc agggaaatgt cggtgcgcga
    12121 ggtcagcagg ttctcaccat cgatgttgat ctcacccgtg gcgcgctggc cggggtagag
    12181 ctcgaacatg cggttgaacg tgcgcaacag cgtcgacttg ccgcatccgg acggcccgat
    12241 gaacgcggtc accttctttt cccggatggg caggctgaca tcacggatcg catgaaactt
    12301 gccgtagtgg aagttcaggt tccggacatc gatcttgatg gattcatcga ggagcgtggg
    12361 catgtacaga ttccagatca gtttttggtg aaaacggcgt tggcgtcacg agttgttgtg
    12421 tcacgagcta gagccacgaa ttggggtgac gaatcaccgc cgtaccgaaa gcagcgaatc
    12481 atttttttcg aaacaacacc cgagccgtga tgctgatgcc cagtacgatg agtgtgatca
    12541 acgcggcccc cgcccaggcc aggcgattcc agtccgaaaa cgggctcgcg gcatactgga
    12601 agatgatgac cggcaggttg gcgatgggcg cgttcatgtt ccacgacatg aactgattgt
    12661 tcaatgccgt gaacaacaaa ggcgccgtct cgccggatat gcgcgccacc gccagaagca
    12721 cgccggtgac gatacccgac cacgcggccc gatagcagac aaacacgacg atgcgccact
    12781 gcgggcaacc cagggccgcg gcggcctcgc gcagactgtt cggcaccagt tgaagcatgt
    12841 tgtcggtgga tcgcaccacc acggggatca ccaggatgga tagcgcgaat gcccccgccc
    12901 atcccgagta gtggccaacc tgggccacat agaccgcata gatgaacagg ccgatgatga
    12961 tagacggcgc tgagagcagc acgtcgttca gaaagcgcgt ggccggcgcc aaccatcccc
    13021 tgcgtccgta ctcggccaga taagtgccgg ccaggatgcc cacgggcgta ccgatcaggg
    13081 tgcccaccgc cgtcatcatc acactgccca cgatcgcgtt gagcaagccg ccatccgcac
    13141 ctggcggggg cgtcgtctcg gtgaacagcg tccaggacat cgcggccccg cccttgtaca
    13201 acaaggtcaa tagaatccag cacagccaga acagcccgaa caacagcgcc agcatcgaca
    13261 acgtcagcat gacgcggttg acgaatcgac ggcggcgata gacaccgttg ggtcggcctg
    13321 cgccggaatc acgggggttc ataactgtcg actcctcaac gcttgccttc gctcccgccg
    13381 agacgggaca gaagcacctt ggacagcgcc aggacgattg tggtgatcag aaacagaatc
    13441 agccccatct ccagcagggc cgacttctgc agcccgcccg cctcgttgaa ttcgttggcc
    13501 aacgcggatg ctatggaatt ggacggcgag aacaacgagg acggcagggt gaaggcattg
    13561 ccgatgacga acgtcaccgc catggtctcg cccagggctc ggcccagccc cagcatgatg
    13621 ccgccaatca ccccgttctt cgtatagggc agcaccacct tccacatcac ttcccaggtc
    13681 gtgccgccaa gaccgtaggc ggattccttc agcatgggtg gcaccacctc gaacacgtcg
    13741 cgcatgacgg cggtgatgaa aggaatgacc atgatggaca ggatgaggcc cgcggtgaac
    13801 acgcctatgc cgaacggcgg gccggaaaac acgtcgcgca acaccgggac gtcgtggaac
    13861 agccagatca gtccgggttg cacatactgc tggaacaacg gcacgaagac gaacaacccc
    13921 cacataccat agatgatcga gggaatggcc gccagcagtt ccaccgccgt tcccagcggg
    13981 cctcgcagcc agcgtggcga cagttccgtc aggaacatcg cgatcccgaa agacacaggc
    14041 accgcgatga gcagcgccag gaacgacgtg agcaacgtgc cgacgaccgg caccagcgcg
    14101 ccataatgat ggcgcaccgg attccagtca ttggtccaga gaaacgccag cccgttcttc
    14161 acaatggaat cgcggctgcc gtaaaccaat gccaccatga tggcggccag caaggcgaaa
    14221 accaggaacg cgaacgcgcg cgtcacgttc ctgaacagca catcgctcac gccgctgcgg
    14281 gatgtattca ttgggccagg gtcttaagct atttccagac cgccgagccg tcggccgcct
    14341 tgatctgcgt cttccatgcc tgggtgatct tcgacgtcac ctcggcgggc agcggcacat
    14401 aatccagcgc ctcggccgcc ttggcgccat tcttgaatgc ccagtcgaag aacgccagca
    14461 cctgccgggc cttctcggga ttctcctgtt tcttgtgcac caggatgaag gtggcggacg
    14521 tcacgggcca ggaacgggct ccgggctcgt cggtcaggac cacgcccatt ccgggcgcac
    14581 tgttccagtc ggcgttctcc gccgcggcgg caaacgcgcc ttgggtgggc tgcacgaact
    14641 ggccgtcgcg attcttcaaa ccaacccagg acaagtggtt ctgcttggcg taggcatatt
    14701 cgacgtaacc gatggcatta tgcaactgcc gcacatatgc cgccacccct tcgttgccct
    14761 tgccgccctg gcccaccggc catttcacgg ccttgccctc gcccacctgc gatttccagg
    14821 ccggcgatac cttggacagg taattggtcc agccaaacgt ggtgcccgaa ccgtctgagc
    14881 gatgcacgac gatgatcgcg gcacccggca gcttgatacc gggattgagc gcggcgatgg
    14941 ccgggtcgtt ccacatcttc accttgccca ggaagatgtc agccagcacc ggccccgaca
    15001 gcctcaactg ccccggcgca atgccctgca cgttgacgac cggcaccgtt ccgccgatga
    15061 ccgccgggaa ctgcagcaaa ccgttctttt ccagatctgg ccctttcatg gggtcgtccg
    15121 aagcgccgaa atcaaccgtc ttggcgatga tctgctgctg cccgccgcca gagccgatgg
    15181 actggtagtt gacccggttt cccgtggcct tgttgtagag cgtggcccat ttggcgtaga
    15241 ccggatacgg gaacgaggca cccgccccag tgatgtcggc ggcctgggca gccaggacaa
    15301 cgctactggc ggcgacgccg gcaaccacct tcaacactac tttcaaattc atcaaatgcc
    15361 cttttggagt cattgggaag cggccggtac acatcgcgga tatccggttt ggaatcgaca
    15421 ctcccggcac aggcgtcatg ataggggcac tctgtgacaa cttcatgaca cactcatttc
    15481 cggcctcgaa cacaagccgg gcaatccgat gaaaacggag gggatctcag cactctggct
    15541 ctgttcgtgc gtcggggccc accctggaac ccaggctccg catgagggag tcatgcaggt
    15601 tgaacgccgg ccgccgatta tgcactcgcg cgtaggcacc gtccgcctgg cagcgccagg
    15661 caagctggtt gtcccgcagc gcgctggtca aggcttcgtt gatgacgcgt ttcttcagcg
    15721 tcttgtcgag caccgggaac cccacctcga cacgccggaa gaaattgcgg tccatccagt
    15781 ccgccgaggc cagatacacg ttctcggcgc catcgtcata gaaatagaac acgcgtgcgt
    15841 gttccaggaa tcggccgatg atggacgtca cgtggatgtt gtcggacagc ccgcgcacac
    15901 ccggccgcag cgcgcaggcg ccccggacga tcaggtcgat cttcacgccc gcctggccgg
    15961 ccttgtagag ctcctcgacg atgatgggtt ccagcagaga gttcatcttc gccatgatgc
    16021 gggccttccg gcccgcccgc gcatgggccg cttcggcctt gatgagcgcg accatggtct
    16081 catgcagtgt gaacggcgat tgcagcagca gcttgagtgg acggcgcgca cccaggccgg
    16141 tcagcaacga gaacaccttg tccatgtctt cgcaaatggc gggattcgcg gtcagcaggc
    16201 cgaagtccgt gtagagcttg gctgtgcgcg ggtggtaatt gcccgtgctg aggtgtccat
    16261 agcgccgtat gcggccctgc tcacgccgca acagcagcgc catcttcgcg tgcgtcttgt
    16321 gcgccaccac gccgtagctc acatgcgctc caacagcctc gagcttggcc gcccagttga
    16381 tgttggtctg ctcgtcgaac cgcgccatca gctccaccac gacggtcact tccttgccgg
    16441 cccgggcggc cagcaacagc agacgcatca attcggaatc ctcaccggtg cgatagatgg
    16501 tctgcttgat ggcgacgaca tccgggtcat gcgccgccgc ggtaaggaaa tctatgacag
    16561 gctggaacga ctgatagggg tggtgcagca ggtggtcgcg ctgggcaatg gcccggaaca
    16621 ggcccgccgg atcgccgccc agcgtatcga acggtgaggg gatggccggc tcgaatgcgg
    16681 ggaacgagag ctctggacga tcgaccagat cacacatttg cagcagccgt gccaggttca
    16741 ccggcccact gacccggtag gtatcctgag gttccagcac gaactcgcgc tgcaggaagg
    16801 attcgagact gggtggcatg ccgtggtcga tttcgagccg cacactggca ccgaaattgc
    16861 gttgcgacaa ctccccctgc aacgcctggc gcaggttggt gatttcctcc tcgtcgacgt
    16921 agaggtcgct gttgcgcgtc acccgccatt gatagcagcc cttcacatcc agcccgggaa
    16981 acagttcgcc cacgaacgcg ctgatcaaag tggtcagcag aatataaccg tcggagatgc
    17041 cggccacctc gggcggcatg cgaatcacgc gcggcaacgc gcgcggtgcc tgcacgattg
    17101 cgatggacgc gcgccgcccg aacgcatcct gcccggacag cgacacaatg aaattcaggc
    17161 tcttgttgta gacccgggga aacggatggg cggggtccag tccgatgggt gtcaacaaag
    17221 gcatgacatc gcggtcgaac gtttcacgcg cccacgccca ctgtgcctcg gtccaggtcg
    17281 tggagaccag cagccccacg ccatgggtca gcaattcggg cagcacatcg ttcatgagca
    17341 ggctgttctg ccgatcgacc agccggtgcg ccgccagttg cacctgttcg aacgcctggt
    17401 ccggcgtcag ggcatcaccg cccaacaccc ccaccggcct gcgttgctgc tctttcaggc
    17461 tggcgatgcg gatttcaaag aattcgtcga gattggaccc cacgatgcac aggtagcgca
    17521 gacgttccaa tagcggtgtt gctcggctct cggccatcgc caacacccgc tcgttgaact
    17581 tgatcaacga caattcgcga ttctgcagcg cgggttcggg caacggggac accatacgac
    17641 caacgatcct ggaatatgaa cggcttattt attgttcttc aatgctactc tacccaagtt
    17701 ggcggcattt cgtccatgtt tttgcggcta tggcgccaca atccaggcta gacaacctca
    17761 cttggcatgc agagcgactc cgggcagctt ttcaaagtgc gcatcgcagg taagcagttc
    17821 cgccctaagg tggcgtgctg tggcgtagac gacggcatcg gccgtggcca gcttatttaa
    17881 cgatgcagtg ccgcagcgag caacacgatc gaagtatcca gcgggatgac aacgcatttc
    17941 tgggtacagg cgatgacggg cttccagatg aacctcgatg gcgtccaggg gtttcagata
    18001 cgtgagttca acaacataca tggtttgtct gcctttggat aaaaagcggg cactcctcag
    18061 ttggaaaact gacaagacgt ttgtgtgtca ggccagcgcc gcgcggatgt cttcccgcag
    18121 cacgggaagc atgtcggtct cgaaccaggg attgcgtttg agccagttgt ggttgtgcca
    18181 tgacgggtgc ggcaggggaa acacgtgcgg cgccgggttc gcgcgataaa gctcgcgcca
    18241 cgcctgcaca gtctgggtga cgccctgacg ggcgcaggcg gcccccaggt gccagcgttg
    18301 cgcatagccg ccgatcacca gcaccagttt caagtcaggc agatgagcga acaatttcga
    18361 gcgccagatc tccgcgcact cccggcgcgg cggcaggtcg ccgccgttgg cgtcgttccc
    18421 gggaaaacag aatcccatgg gcaggatcgc gatgcgggat tcgtcgtaga acacgctttc
    18481 gtcgatggcc atccattgcc tcagccggat acccgaccgg tcgtcgaaag gccgcccgct
    18541 ttcgtggacc cggattccgg gcgcctgact ggcgatgcag atgcgcgccg tgcgcgacac
    18601 ctggatgatg ggccgtggtt cgtggtccaa ccgcgcgccg tagcggggct catcccggca
    18661 gacacgacag gcgcggatgg cgcgcaccag cgcgtcgaac gagtcattct ggctgtgcga
    18721 agctggatcg gacatgtcgt tggctttctt cgtttgcatt ttcttcccca tcccgctcag
    18781 ccgctgtcag cgattcgccc cgatgcggcc gcactaaata cgcgtgagta cacgaccttt
    18841 atgcgccgcg atgtggtcgg tcttgtcgct ctttatttcg tactggggat catcctcgga
    18901 cgcgtgatgc acatgcccct tgtagtcgaa gtcttgcgtg tggatcgcga tgatcgtacc
    18961 ggaaaccctg cccgcctctg aattccagcc cacgtgatcg ccaacgcgga atcgttccgt
    19021 catttcccgt gcgccttgcc gtcgagcaac acatttcgca gcaccaccgc gttgcaatgc
    19081 gcttcgtcat gcgcgctgta gaccagggtg acggtttgcg tcttggccaa cgtgcgcacg
    19141 cgatccagtg cctcggggtt ctgatcgagt tcggccccat agcgctgctg aaactcatcc
    19201 caccgcgcgg gatcatggcc aaaccactgg cgaagctcgg tgctgggtgc gaggtccttc
    19261 atccagtcgt ccagcgccgc cttgtctttg ctgacccccc tgggccatag ccgatcgatg
    19321 agaatacgca cgccgtcatc aggtgaagcg ggttcgtaag cccgcttcac ccggacatgg
    19381 gaagtgggaa cagatgatcg tgacatatgc aagactccca aaaacacgcg ctcgtacctt
    19441 ccccgagacg atagcgcatc ttccccggcg aaaccgcgct tcttctaaga taccgccatc
    19501 cgcccccctt cgcaaagggc tggctcccac aagggtgctg tccaccactg tgggcccacc
    19561 acgctttggc tcaccagccg taaaagcgcg gccacgcctc gacgcggcca tcggggtata
    19621 tggcctggta ttcgggatac tgtgcgccgg tggcacaagc atgattggga aggatgcgca
    19681 atcgtgtgcc aacgggaaag cgccttgcga tgtgggggtc cggtgcgccg gtgctcgaca
    19741 ggatcccgtg ctcttgattt gcgccactca ggacgtagcc ttcgagcacc gcgccatctt
    19801 ccgcgcacac ctggccgaaa ccgaaatcct gcttctgctt ctgtgtgccg cgatcgcggc
    19861 tcatggccat ccaccccgcg tcaacgatgg cccacccctt ctcgccctgg tggccgatga
    19921 cggtggccaa cacgctcaag gcgatatcgt ccattgcgca gacgccgaca ttacgcatca
    19981 ccaggtcgaa gaacacatac accccggccc gcacctcggt gaccccctcc aggtgttccg
    20041 ccgacagcgc cgttggcgtc gagccgacac tgacgaccgg gcacggcagg cccgcgccac
    20101 gcaagcgttg cgcggcccgc accgcgccgg cgcgctcttg ctcggccatg gcccgcaacg
    20161 cggcgtcggt atcacagtca taactcgacc ctgcgtgagt catgacaccg cccaaccgca
    20221 tacccgcctc gtgcaaggcc cgccccacgt ctagcaacga atcctcttcg ggcttgatgc
    20281 cggaacgatg cccgtcggtg tcgatttcca cccacacctc gaactcttca tcgttcagct
    20341 ttccaaagcg tccgatctcc cgcgccgcct cgacgttgtc cgtgatgagc ttcaggtcgc
    20401 agccgcgccg acgcagcgcc agcgcgcgcg ggagcttgtc cggcgccatc ccgacggcgt
    20461 agagaatgtc ggacacacca ctcgcaaaga actgttcggc ctccttcaag gtggaaaccg
    20521 tgatgccctg cgcgcccgcg acgatctgcg cctgcacgac gggcaggcat ttcgttgtct
    20581 tcacatgcgg gcggaaacgc acgccgagcg cattcatgcg cgcctgcatg cgctcgatgt
    20641 tgcgccgcat tcgctgcaca tcgacgagcg cggcgggtgt attcaagtcg tgaatgccgg
    20701 tcatgaatca ggttctctgg ataggtggat gatggcggta taggccgcat tcctttgcgc
    20761 tggcgtgcct cacccagcta tctcgtccga gcgcagccaa cccgtcaggc tccagcgcgt
    20821 ctgctggcaa ggcaacaccg cgtgcggaac cagaccgctg tggaaaacca ccagccgcgc
    20881 gccctgcggc gagatccgcc gcatttcaac attgggggct tccggctcgt acaggcaaag
    20941 atcaccgcca tacatggggt tccattgaag gttcaaatac agcacgatag atatcttgcg
    21001 atgatcggtg tcgcgatgct gatcgatgtg cttccggtac cccgtgccct tgccgtaccg
    21061 cgcgaagtgg aactcctggc tgcgtagccc caggttggtg cgctcgctga gttcctggcg
    21121 gaaccgcgcc atccacgaga aaaacgggtg cctggacagg agcgaacctg gaggcaccca
    21181 gcagaccgtg tcgccacgga tgtccggccg ccgtgcatcg ctcacgccgc ggccgatgcc
    21241 ttccatgtgg aagcagccgt cctgccacaa ctgccagccc agcatcaaca aggtgatgtg
    21301 ccacgacagt ggaatgacgg tgtcggaaac cgcccagccc cgcttgtcca gcgattgaaa
    21361 gatcttgtcg tggtcattca tgaggcttgc cattcccggg gtgaaactgt catgtgcgaa
    21421 tcatctgcgc gccaaaattt taaccccagc tcttttcaaa gcggtggtgc cgatatgcag
    21481 caggtttttg tgctcgacat cacacaagac gccatacgat cggacagacc caagcctctc
    21541 acccaaccaa agcatcgatc tcggcggaag gcttgttcca caaccgccgg ccctctttcg
    21601 ccacgttgac ccgtagcgtc gtctcagcca catccataag ggcgccgtcc agcgtatagc
    21661 caccctgcgc ctgtaaccaa cccaacacgt ccaccagatg ccagatggac gcgctgccct
    21721 catgcaccgg ggcgggaaag gtgtcaggat gagtcagcat caacttgcgc atgttctgcc
    21781 gcgacatgcc cgtggcctct gccacatcag tgaggccaac caagtctggc gccgcctcga
    21841 tgagactggc tgacggcaca gcgcgccgca cgtcggccag cgcgctgtgc acagcgtcct
    21901 cggcgcaggc cgcctcgcgg gtgaatgcca atgccaaccg ccccggctgc ccgatgccga
    21961 ccaaggcatc gtcgcagcca gcttcgccca ggctctcgac caaggcatcc aagtcgtaga
    22021 cgctgctgga aagcaagtat ttcagagtga aggtatattc catcgtgcta ttcctctgcg
    22081 ccgccttcgg tttcacgcaa cttgcgatgc ctgatgcagt tgtccacgac acgtcgcaag
    22141 gcgcgggcat ggttgcccgg atttttgggc gtactccata cgctgacaat gcagaactcg
    22201 ccgcaacgac acgcttcatc gtcatagggg caatagaccc gcccccaggc atggctgtcg
    22261 cccacttcga cacgccaacc ctgcctttcg atgtaccgaa gcgcttcctc aatctctttc
    22321 ttcggatgga ggggacgact catcgatgag ctccagtatg ggagtgacga cattagttgt
    22381 caactgacaa cctgtgtgtt tcagcctgtc gacgcaaaag tatgccgcac cctggaaggg
    22441 gtcgaagatc gttgtggttg attgatcaga tggggattac ccacaacaag cagagccatc
    22501 gactcgacgc gcacaaccgc agaaatcctc cgaaacaaca atatcttgca cggcatgcgc
    22561 accgttcact cacgcaggag tagagtcgaa attgccattc gccaccacgc gaaccacggc
    22621 tgctccacgc gatgccggag ctcatactga acatccaccg acttcagcga aacctatgaa
    22681 caccactgac tcagccgcca tttcggcggt caaccggttc gggctgggcg gcaagcccgg
    22741 cgaactcgaa ggtgtgaaac aaccgcggga atggttgttg tcccaggtcg cgcgcggccc
    22801 cgatggcgcc gcgctgttcg tgggcttgcc gtccagcctc gactacctgg gcgaggaagc
    22861 gcggttgcag cagactcgcc acgaactgaa acaaaaagag cgatcccggc gcgacttcct
    22921 gacgggccgt ccatcaacag aggacgcggc ggacggcgcc gccgccctga agcaagcctt
    22981 gaaaccctac cgcgaggagg agcggaagga cgtgctggcc gaggccgccg cgcgctatca
    23041 ggtggcggcg accaccgaca cgccgttcgt ggaaaggctg gtgcatttct ggtccaacca
    23101 cttcgccgtt tccaccgaca agggtccggt gcgattgctc gccgcgccga tggaacgcga
    23161 ggcgatccgc ccgcatgtca ctggccgttt cgccgatatg ctgctggccg ttgaaaccca
    23221 cccggccatg ttgctgtacc tggacaacat acgctcagtg gggccgaact ccccgcttgg
    23281 ccggcgcatc gcgaaacgcc tggcgcgcaa gggggcacgg gacgaaggca aggcgggcgg
    23341 gctgaacgaa aacctgggcc gtgaagcgat ggaactgcac acggtggggg tgaacgccgg
    23401 ctatacccag gccgacgtca ccgagttctc gcgcgccttg acgggctgga gcatcccctt
    23461 tccacgtgat ctggtcggcg actatccgct gcagtcggcc ttcatgttcc gcgccaacgc
    23521 gcatgaaccc ggcgcgcgcc gggtgatgga gcacacgttc ccggaaggcg gattcgagca
    23581 aggccgcgcc attctggaat tcctggcgca tcaacccgcg accgccttgc atctgtca
//
LOCUS       DASTNK010000064        12590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_970845, whole genome shotgun sequence.
ACCESSION   DASTNK010000064 DASTNK010000000
VERSION     DASTNK010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12590
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970845"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="VFR20_05070"
     CDS             <1..139
                     /locus_tag="VFR20_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0229921.1"
                     /translation="GLFARLMYIGLHLMHHAALLGLLRTGSLALGRLLLKRGAPRVKL
                     H"
     gene            201..875
                     /locus_tag="VFR20_05075"
     CDS             201..875
                     /locus_tag="VFR20_05075"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU0229922.1"
                     /translation="MTYHAIMASPLGDILLCADHQGLSGLYFVGQGDCPQVSGLPEQP
                     MPRADRPSDGMMAGQAIRDFRAHRISTGDMFSRPGCATRALAARSGSADDAPPRILKE
                     GASSDVQSLFLMAQRELAEYFAGRRTSFDVPLMLQGTEFQKKIWRALLAIPYGTLSSY
                     GEVARSAGFSARHGRPAGAAVGRNPVTIIIPCHRVVSGTQALTGYTGGLERKFALLEL
                     EGFSLF"
     gene            967..1395
                     /locus_tag="VFR20_05080"
     CDS             967..1395
                     /locus_tag="VFR20_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEU0229923.1"
                     /translation="MWFWFGFAVLALIGEVMTGTFYLLLFAVGLAGAGIVALLGGDLA
                     WQIVTVAVVTLLGLLILRRSGVLKKREVNSARNADVNLDIGQTVEVEAWSANDTARVW
                     YRGAYWQAVPAPGAARHPGAYRIVEIQGSTLYLEPVIPTH"
     gene            1457..2389
                     /locus_tag="VFR20_05085"
     CDS             1457..2389
                     /locus_tag="VFR20_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HEU0229924.1"
                     /translation="MNMDFSTLVLVIVVVLVFLIVVKAIAIVPQQHAWVIERLGRFDR
                     TLSPGAGFVIPFIERVAYKHSLKEIPLDVPSQICITRDNTQLQVDGVLYFQVTDPMRA
                     SYGSSNYISAITQLAQTTLRSVIGKMELDRTFEERDAINSTIVSALDEAAHSWGVKVL
                     RYEIKDLTPPAEILRAMQAQITAEREKRALIAASEGRRQEQINIATGEREAFIARSEG
                     EKQALINKAQGEATAVVAVAEATARAITQVAESVRQPGGMEALNLRVAEQYVDAFSQV
                     AKAGNTLIVPANLADMGTLVASAMSIVKSTGKTA"
     gene            complement(2460..2912)
                     /gene="smpB"
                     /locus_tag="VFR20_05090"
     CDS             complement(2460..2912)
                     /gene="smpB"
                     /locus_tag="VFR20_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU0229925.1"
                     /translation="MSIIDNRKATHDYFIEDRFEAGLVLEGWEVKAIRAGHVQLRESY
                     VIVRDGEIWIIGMHISPLPTASTHINPNATRTRKLLLKAEEINKLIGKVEQRGYTLVP
                     LNLHYKNGRVKLDFGLGRGKKLHDKRDTARDKDWAREKERVMKHDMRH"
     gene            3128..3562
                     /locus_tag="VFR20_05095"
     CDS             3128..3562
                     /locus_tag="VFR20_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEU0229926.1"
                     /translation="MHTVHRSVLVPYSRAQMFDLVADVEKYPEFMPWCGGTRVLERDE
                     HGMRASVTISFAGIRQAFTTQNRHEYPELITLDLVDGPFSLLTGRWEFNELAPQACKV
                     LFTMEYAFSSRALEAVVGPVFNRIASSFIDSFTRRAQDCYGQ"
     gene            3552..3863
                     /locus_tag="VFR20_05100"
     CDS             3552..3863
                     /locus_tag="VFR20_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEU0229927.1"
                     /translation="MANSGTVRVTLVYARPGFVWRRALRLPAGSTVWQAIQKSGFMDQ
                     VPDQGGIVSCGIFGQSCGNDRVLADGDRVEILRPLVFDPMESRRRRAAHRAARALPGK
                     R"
     gene            4082..5236
                     /locus_tag="VFR20_05105"
     CDS             4082..5236
                     /locus_tag="VFR20_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0229928.1"
                     /translation="MDFHLSDEQQAFALAAREFAQGELAPNAARWDEESIFPTETFAQ
                     AGALGFCAIYAPESIGGLGLPRLDATLVFEEMAAVDPSTTAFLTIHNMATWMIGSWAT
                     ESVRGAWGPVLAAGEKLASYCLTEPGSGSDAASLSTRAEKKGGAYILNGAKAFISGAG
                     DTDVLVVMARTGGPGARGISAFVVPGDMPGITYGRKEHKMGWNSQSTRPITFENVEVP
                     AENLLGSEGEGFKFAMKGLDGGRINIATCSVGAAQGAYDAARRYMGERKQFGSPLADF
                     QALRFKLADMATHIVASRQMVRLAASRLDAGDPSAATYCAMAKRFATDLGFQVCLDAQ
                     QLHGGYGYLKDYPLERLVRDTRVHQILEGTNEIMRVIIARQLLDEGTDIR"
     gene            5233..6366
                     /locus_tag="VFR20_05110"
     CDS             5233..6366
                     /locus_tag="VFR20_05110"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU0229929.1"
                     /translation="MNAPVLYDVVATAGPHKVGVATLNSPQTLNGLSLAMCESLAAQL
                     AEWESDDTIALVVLRGAGEKAFCAGGDLHGLYAGMRDNASGRAWDNLYARRFFDVEYR
                     LDYQIHTYSKPVLCWGSGIVMGGGVGLMMGASHRVASDTTRFAMPEITIGLFPDVGGT
                     WMLSRLPAGIGLFLALTGAQLGASDCRHLGLANYVLESHRWPELMERIQSEEWAPSRA
                     QNDDRLHRILTALQAADLAPGPLQTHYTEIQSWCLSPNLETVGANLAALASHDDPWLK
                     RAASTFIAGSPGSARLCFALLRRTRNLSLAEAFRQEYIVSLQCGVQGDFQEGIRALLI
                     DKDKKPAWRPASLAEADDAWVARFFAPSWPRGSSHPLADLGNA"
     gene            6462..7346
                     /gene="mmsB"
                     /locus_tag="VFR20_05115"
     CDS             6462..7346
                     /gene="mmsB"
                     /locus_tag="VFR20_05115"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HEU0229930.1"
                     /translation="MSRVAFIGLGHMGGPMALNLIKAGHELHVHDLVPQAIKAAVDAG
                     AQAADSAASAVRGADAVVTMLPASKHVEALYLGESGLLAGIAPGTLVMECSTIAPASA
                     RKVAEAAQARKIPMIDAPVSGGTGGAAAGTLTFIVGGENGALEAARPYLEVMGKNIFH
                     AGGAGAGQVAKICNNMLLGILMAGTSEALALGVASGLDPKVLSDIIAKSSGRNWATEL
                     YNPWPGVMEHAPASHDYAGGFGVDLMLKDLGLAAEAALQARASVPLGELARNLYSLHS
                     AQGNGALDFSSILNLFKK"
     gene            complement(7375..8430)
                     /locus_tag="VFR20_05120"
     CDS             complement(7375..8430)
                     /locus_tag="VFR20_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEU0229931.1"
                     /translation="MNSHAPLEASLDDLALLEVSGPDAATFLQGQLTQDIASSNPARA
                     QLAGYCSPKGRLMASMIVWQGADPPSAFFILLKQDIAAAFAARLAKFVLRAKVSIKPL
                     PARIDGVLLPMTEGALESLAMSDAGSAQLVTYDDDQASELPTPPSGVFRPYDITRSNS
                     GIWVAAPSGHPALQRWWRIIPDASASASSIGANRSSQPLHSALSVPASCWQTQDIAAG
                     LGWVVSATHELFIPQTLNLDLIGGLSFTKGCYPGQEVVARSHYRGTIKRRMAHGLIPA
                     ANQQAEELPGSDIYDSTHPDAPCGRVINAVNVSGAGHILFEAALTDLGQADFRLGAAD
                     GPAITLESLPYDITATA"
     gene            8543..9550
                     /gene="mltG"
                     /locus_tag="VFR20_05125"
     CDS             8543..9550
                     /gene="mltG"
                     /locus_tag="VFR20_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU0229932.1"
                     /translation="MRRMKAVLGYGVLAAIIVVAAAAGFAWHWVYSPVRMDANPIDYT
                     VEPGSSPRIIARLMNKQGIHVNETGFVLLARLSGRAKGLKAGAYETQAGDTPWKLLER
                     MANGDMTQARLTLVEGWTYQRIRQALRDDPSVKQTLQGVSDKELLSRLGSDKTNPEGL
                     FFPDTYVFAPGTSDFDILRRAYRAEQSLLATLWADREPGLPLTSPYQALILASLIEKE
                     TGHSADRRRVAGVFVNRLRLNMPLQTDPSVIYGMGDAYHGKLHKKDLETDTPWNTYTR
                     TGLPPTPIACVGKASLLAALHPEAHKYLYFVSRGDGTSEFSEDLASHNRAVAKYILGK
                     H"
     gene            9550..10209
                     /gene="tmk"
                     /locus_tag="VFR20_05130"
     CDS             9550..10209
                     /gene="tmk"
                     /locus_tag="VFR20_05130"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU0229933.1"
                     /translation="MTQERRGCLITLEGLDGAGKSTHVQWLVDCLRSKGMSVLSTREP
                     GGTALGEQLRQMVLTQPMRLETETLLMFAARCEHIHTVIEPALADGRWVVCDRFTDAT
                     YAYQGGGRGLGGAAVRALEQWVHPGLQPDLTLLFDVPLEVARQRLARGRDLDRFEQEG
                     AGFFERTRNAYHERATAHPGRVRIIDSTLGVEQVRESLARQLDILFDKRRASSTGRSE
                     S"
     gene            10206..11249
                     /gene="holB"
                     /locus_tag="VFR20_05135"
     CDS             10206..11249
                     /gene="holB"
                     /locus_tag="VFR20_05135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU0229934.1"
                     /translation="MTQPPRFLPWQFDMARTWLGARERFAHAWLVHGLAGTGKRQFAL
                     AAAASLLCEQPEHGLACGHCTACQWFASGNHPDFRRIRPDAVALEEGAEQDDTEATGA
                     GASKTPSAEIRVDQLRSLGTWFNTATHRGGWRVAALYPAHALNTISANALLKVLEEPP
                     EHTVFLIVADAPDRLLPTLVSRCRRLPVPVPPRQDSIAWLKSEGVADPGQWLASAGGA
                     PLLALRSARAADTPCPDWLSGFLMALAEGGVADVGPLADLLDKTPPEEWLDGLQRLFM
                     DLALAGASVPLRYYPTLEGPIRIVAERASLARLTQAGHWLAKQRRFARHPLNARLLVH
                     ATLDRLAVACRPV"
     gene            11303..12091
                     /locus_tag="VFR20_05140"
     CDS             11303..12091
                     /locus_tag="VFR20_05140"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU0229935.1"
                     /translation="MFVDSHCHLNFPDLAANLPDILARMARNQVGHALVVSVNLPDWP
                     GLMALVGPHANLYASVGVHPDYEDTPEPSVDELVGLAAQPKVVAIGETGLDYFRLAEP
                     LEWQRERFRTHIRASRASRLPLIIHTRVASADTLRIMQEEGADDIGGVMHCFTESWEV
                     AEAAMAQNFYISLSGIVTFKSAKALQDVATRVPLDRLLIETDSPYLAPVPHRGSVNDP
                     SKVIHVAEKIAELRGVPVATIAEHSTNNFFKLFNKINRNIEVKQ"
     gene            12267..>12590
                     /locus_tag="VFR20_05145"
     CDS             12267..>12590
                     /locus_tag="VFR20_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEU0229936.1"
                     /translation="MASAHAAVPDGWWTDIANNRVSDIEGMLLQGANPNAIDPHGDPS
                     IMFAVRRKAWDVFDALAADPQTNVNLANRLDETPLMYLAIVGQTARARTLIQRGAKVN
                     RLGWTP"
BASE COUNT         2274 a         3818 c         4053 g         2445 t
ORIGIN      
        1 agggctgttc gcgcgtttga tgtacattgg tttgcacctg atgcaccacg cggcgctgct
       61 cggccttttg cgcacgggct cgctggcgct gggccgacta ctgttgaagc gcggcgcgcc
      121 tcgcgtgaag ttgcactagg acccgcccac cccaatacca gtccaccttg ccgcattgcc
      181 gcgaatccgg aatattttca atgacatacc atgcgatcat ggcgtcgcct ctgggcgaca
      241 tcctgctttg cgccgaccac cagggcttga gcggcctgta tttcgtgggc cagggggact
      301 gcccacaggt gtcgggactg ccggagcagc ctatgccgcg tgccgaccgc ccatccgatg
      361 gcatgatggc gggccaggcg attcgcgatt tcagggcgca tcgaatcagc accggcgaca
      421 tgttttcccg gccgggttgc gcgacgcgtg cactggctgc gcgatccgga tcggcggatg
      481 atgcccctcc gcggatattg aaggagggtg cgtcaagcga cgtccagtcc ttgttcctga
      541 tggcgcagcg tgaactggcg gagtatttcg ccggacgccg gacgagtttc gatgtgcccc
      601 tgatgctcca gggaaccgaa ttccagaaga aaatctggcg ggcgttattg gccattccgt
      661 atggcacctt gtcgtcatac ggtgaagtcg cccgttcagc cggattctcc gcgcgtcatg
      721 gccgcccggc gggtgcggct gtggggcgca atcctgtcac tatcatcatt ccctgccatc
      781 gcgtcgtgtc gggtacgcag gctttgacgg gctatacggg tggcctggag cgcaagttcg
      841 cattgctcga actggaaggc ttttcgttgt tctgagagta gggcgcggag tgctaagcat
      901 gcggctacaa tgagccgcgc gatggggttc cgacggaatg ccgtttgtca gagggggatg
      961 attgtcatgt ggttctggtt cggtttcgcg gtgctcgcac tgatcggcga ggtcatgaca
     1021 ggtacgttct acctgttgtt gttcgctgtc ggactggccg gcgcaggcat cgtcgctttg
     1081 ttgggtggag acctggcttg gcagatcgtg acggtcgccg tggtgacgct gttggggctg
     1141 cttattctgc ggcgctcggg cgtgctcaag aagcgcgaag tcaattccgc ccggaacgcc
     1201 gatgtcaacc tcgacatagg acagaccgtc gaagtggaag cttggtccgc gaacgacacc
     1261 gcgcgtgtct ggtaccgtgg tgcctattgg caggccgtgc cggctccggg cgcggcccgg
     1321 catcccggcg cataccgtat cgttgaaatc cagggttcca ccttgtattt ggaacccgtc
     1381 attcccaccc actgagcgcc ggataaccgg ccgctcgttt tgccgttcgg cacaatataa
     1441 agaaaaggtc tcgatcatga acatggattt ttccacgctg gttctggtca tcgtcgtcgt
     1501 gctggtcttc ctgatcgtcg tcaaggccat cgccattgtg ccgcagcagc atgcatgggt
     1561 gatcgaacgc ctggggaggt tcgatcggac gctgtcgccg ggcgcgggct tcgtgattcc
     1621 tttcatcgaa cgggtcgcct acaagcactc cctgaaggaa attccgctgg atgtgccaag
     1681 ccagatctgc atcacccgcg acaacacgca gttgcaggtc gacggcgttc tgtatttcca
     1741 ggtgaccgat ccgatgcgcg cgtcgtacgg ctcgtcgaat tacatctcgg ctatcacgca
     1801 gttggcccag acgacattgc gttcggtcat cggcaagatg gaactcgacc gcacgttcga
     1861 agagcgcgat gccatcaaca gcaccatcgt ttccgcgctc gacgaggcgg cacacagttg
     1921 gggcgtgaaa gtgttgcgtt acgaaatcaa ggatctcacg ccaccggccg aaatcctccg
     1981 ggccatgcag gcccagatca cggccgaacg cgagaagcgc gcactgatcg cggcatctga
     2041 aggacgccgc caggagcaga tcaatatcgc cactggcgaa cgcgaggcgt tcattgctcg
     2101 ttccgaaggt gagaagcagg cgctcatcaa caaagcccag ggcgaagcga cggcggtcgt
     2161 ggcggtggcc gaagccacgg cgcgcgccat cactcaggtg gcggaatcag tgcgccagcc
     2221 cggtggcatg gaggccctga acctcagggt ggctgaacag tacgtcgatg cattctcgca
     2281 ggtggccaag gcgggcaaca cgctgatcgt ccccgccaat ctggcggaca tgggaacgct
     2341 ggtcgcctcg gccatgagca tcgtgaagag tacgggtaaa accgcttgac gcttgccgat
     2401 tgaaagtggt cggctccccg cggtgccgct actgacaccg agggccgaag aggtggcact
     2461 cagtgccgca tatcgtgttt catgacccgc tctttctcgc gcgcccagtc cttgtcgcgc
     2521 gcggtgtcgc gtttgtcgtg cagtttcttg ccgcggccca ggccgaaatc gagtttgacc
     2581 cgcccgtttt tgtagtgcaa gtttagcggc accaacgtat agccgcgttg ctcgaccttg
     2641 ccgatgagct tgttgatttc ctcggccttg agcaacagct tgcgggtgcg ggtggcattg
     2701 ggattgatgt gcgtcgatgc ggtgggcagc gggctgatgt gcatgccgat gatccagatt
     2761 tcaccgtcgc gcacaatgac gtacgactcg cgcagttgca cgtgcccggc ccggatggcc
     2821 ttgacttccc acccttcgag cacgagcccc gcttcgaagc ggtcctcgat gaagtaatcg
     2881 tgcgttgcct tgcggttgtc tatgatgctc atgaatcagg gggctgccgt atgtgtgtgc
     2941 cggtagtatg tgtaccggta tgtgtgtact gatatgtgcg tactggtatg tgcgtgccgc
     3001 ccgtgtcaac aacgcgtgga cgtgcaacca aacgggtgcg cgcgcaggca gtaaaaacac
     3061 gcccaccggg ctgtgtgtgg cgggcaagaa ggtaaaatcc atacattcta tccattaata
     3121 ttgcttgatg cacaccgttc atcgctccgt tctcgttccg tacagccgcg cccagatgtt
     3181 cgacctggtg gccgacgtcg aaaagtatcc cgagttcatg ccctggtgtg gcggcacccg
     3241 ggtgctcgaa cgtgatgaac acggcatgcg ggcgtcggtc accatcagct ttgccggcat
     3301 ccggcaggcg ttcaccacac agaaccgtca tgaatacccg gagctcatca cactcgatct
     3361 tgtcgatggc ccgttttccc tgttgactgg gcgctgggaa ttcaacgagc tcgctcccca
     3421 ggcctgcaag gtgctgttca cgatggaata tgcgttttcc agccgcgcgc tcgaagcggt
     3481 cgtgggcccg gtgttcaacc gcatcgccag cagtttcatc gattcgttca cccgccgcgc
     3541 ccaagactgc tatggccaat agcggcaccg tgcgcgtgac cctggtctat gcccgacccg
     3601 ggttcgtgtg gcgccgcgcg ttgcggctac cggcggggtc gacggtgtgg caggcaatcc
     3661 agaagagcgg gttcatggat caggtgcctg accaaggtgg catcgtgtcc tgtggcattt
     3721 tcggccagtc atgcggcaac gatcgtgtgc ttgccgacgg tgatcgggtg gaaatcctgc
     3781 gccccctggt cttcgatccc atggagtcca ggcgtcgccg cgcggcgcac cgtgccgcgc
     3841 gcgcacttcc cggcaagcga tagtgccgat ctcctgcgca aatgaatcgg gccacccgcc
     3901 cgcttggcgc gtgcatgccg ggcacggacc ccgacattga tggtggcccc gcagtccgcg
     3961 ggcatgctac attgttggcc gcaccgctcg accgtggtgg tgcccgatcc atccggccac
     4021 gaaccgggct ggaaaaaaat atactaagtg catcaccacg caccgaagga gatattccga
     4081 catggatttt catctcagcg atgaacaaca ggcgttcgcc ctggccgccc gggaattcgc
     4141 ccagggtgag ctggccccca atgcggcccg ttgggacgaa gagtcgatct tccccactga
     4201 aaccttcgct caggctggtg cgctggggtt ctgcgccatt tacgctcccg agtccatcgg
     4261 cgggctgggt ctgcccaggc tcgacgccac gctggtgttc gaagagatgg ccgccgtcga
     4321 tccctccacc acggcgttcc tgacgattca caacatggcg acgtggatga tagggtcctg
     4381 ggccacggaa tccgtacgcg gggcctgggg gccagtgctc gcggccggcg agaagctcgc
     4441 ctcgtattgt ctgactgagc cgggctcggg ttccgacgcg gcgtcgctgt ccacgcgcgc
     4501 cgaaaagaag gggggcgcct acatcctcaa tggcgctaaa gcgtttatct cgggcgcggg
     4561 cgacaccgat gtactggtcg tcatggcgcg tacgggcgga cccggggcgc gaggcatctc
     4621 ggcctttgtc gtgcccggcg acatgccggg aatcacctac ggccgcaagg aacacaaaat
     4681 gggctggaac agccagtcca cgcgccccat caccttcgag aacgtggaag tgcccgccga
     4741 gaatctcctg ggctcggaag gtgagggctt caaattcgcc atgaaagggt tggacggcgg
     4801 gcgcatcaac atcgccacct gctcggtggg cgcggcgcaa ggcgcctatg acgccgcccg
     4861 gcgctatatg ggtgagcgca agcaattcgg atccccgctg gctgacttcc aggccctgcg
     4921 gttcaagctc gccgacatgg ccacgcatat cgtggcgtcg cgccagatgg tgcgcctggc
     4981 ggcgtcgcgc ctcgatgcgg gcgaccccag cgccgccaca tactgcgcca tggccaagcg
     5041 cttcgcgaca gacctgggct tccaggtttg cctggatgcg cagcaactgc atggcggcta
     5101 cgggtatctg aaagactatc cgctggaacg cctggtgcga gacacccggg tgcatcagat
     5161 tcttgaaggc accaatgaaa tcatgcgcgt catcatcgcg cgccaattgc tcgacgaggg
     5221 aaccgatatc cgatgaacgc cccggtactt tacgacgttg tcgccaccgc cggcccgcac
     5281 aaagtgggtg tggccacatt gaacagcccg caaacgctca acggcctgtc gctcgccatg
     5341 tgcgaatccc tggccgcgca actggccgag tgggagtccg acgacaccat cgcgctggtc
     5401 gtgctcaggg gggcggggga aaaggcgttc tgcgccggcg gcgacttgca cggcctgtat
     5461 gcaggcatgc gggacaacgc cagtggccgg gcatgggaca atctctatgc acggcgcttt
     5521 ttcgacgtgg agtatcggct cgactatcag atccacacct attccaagcc ggtgctgtgt
     5581 tggggcagtg gcatcgtcat gggcggcggc gtcggcctca tgatgggcgc cagccacagg
     5641 gtggccagcg acacgacccg tttcgccatg cccgaaatca cgatcggcct gttccccgat
     5701 gtgggtggca cctggatgct gagccgtctg ccggctggca ttggcctgtt cctggcgctg
     5761 accggcgcgc aattgggggc cagcgactgc cgccatctgg ggttggccaa ttatgtgttg
     5821 gaatcccacc gttggccgga actgatggaa cgcatccagt ccgaagaatg ggcgccttcg
     5881 cgtgcgcaga acgacgatcg acttcatcgt atcctgaccg ccttgcaagc ggccgacctc
     5941 gccccggggc ctttgcagac ccactacacc gaaattcaga gctggtgcct gtcgccgaac
     6001 ctggagacgg tcggcgcgaa cctggcggcg ctcgcctcgc acgacgatcc ctggctcaag
     6061 cgcgcggcaa gcacgttcat cgcggggtcg ccggggtcgg cccgcttgtg cttcgctttg
     6121 ttgcggcgca ctcgcaatct ttcgctggcc gaggctttcc gacaggaata catcgtgtcg
     6181 ctgcaatgcg gcgtgcaagg cgatttccag gagggcatcc gggccttgct catcgacaag
     6241 gacaagaaac cggcctggcg acccgccagc cttgcggagg ctgacgatgc ctgggtcgcg
     6301 cgctttttcg cgccgtcgtg gccacgaggt tccagccatc cgctggccga tcttggaaac
     6361 gcataagcca gggcgcctgg ccgcatagac agtttttcat cagaacaggc ggcccgcgtc
     6421 gtcgacacga accgtccatc accgaccaaa aaggagacat catgagtcga gtcgcattca
     6481 tcggtctggg gcatatgggt ggccccatgg cattgaactt gatcaaggct gggcatgagc
     6541 tgcatgtcca cgatctggtg ccgcaggcca tcaaggcggc agtggatgcc ggtgcgcagg
     6601 ccgccgattc ggcggcgtcc gccgtgcgcg gcgcggatgc ggtcgtgacc atgctgccgg
     6661 ccagcaaaca cgtcgaagcg ctataccttg gcgaatccgg cctgcttgcc ggcatcgcgc
     6721 ctggaaccct cgtcatggaa tgcagcacca tcgcgccggc ctcggcgcgc aaagtggccg
     6781 aggccgccca ggcccgcaag attcccatga tcgacgcccc ggtgtccggc ggcaccggcg
     6841 gcgcggcggc gggcaccctc acgttcatcg tgggcgggga aaacggcgcc ctcgaggcgg
     6901 cgagacccta tctggaagtc atgggcaaga acattttcca cgccggcggc gccggggcgg
     6961 ggcaggtggc caagatctgc aacaacatgc tgctgggtat cctcatggcc ggcacctccg
     7021 aggcgttggc gcttggcgtc gcgagcgggc tcgaccccaa ggtgctttcc gacatcatcg
     7081 ccaagagctc cggacgcaac tgggccacgg aactgtacaa cccgtggccg ggcgtcatgg
     7141 agcatgcgcc ggcctcgcac gattacgcgg gggggttcgg tgtggacctg atgctgaagg
     7201 atctgggcct ggccgccgag gcggcgctgc aggcccgggc ctcggtgccg ctgggcgaat
     7261 tggcgcggaa cctgtattcc ctgcatagcg cccagggcaa cggtgcgctg gatttctcca
     7321 gtatcctgaa cttgttcaag aagtaagaaa acacgacatc acggcggcgg ctcttcaggc
     7381 ggtcgctgtg atgtcgtagg gcaggctttc cagggtgatg gccgggccat cggcggcacc
     7441 cagacgaaaa tcagcctgcc caaggtcggt aagcgcggct tcgaacaaaa tgtgcccggc
     7501 accgctcacg ttgacagcat tgatgacgcg gccgcacggt gcgtcggggt gtgtgctgtc
     7561 gtagatgtcg gatccgggca gttcttccgc ctgttggttg gccgctggaa tgagcccgtg
     7621 ggccatgcga cgcttgatgg tgccccggta gtggctgcgc gccaccacct cttgacccgg
     7681 gtagcaaccc ttggtgaaac tcaacccgcc gatcagatcg agattgagcg tctggggaat
     7741 gaatagctcg tgggtcgccg ataccaccca gcccaacccg gccgcgatgt cctgcgtctg
     7801 ccagcacgag gccggcacgg acaaagcgct gtgcaagggt tgagaggagc ggttggcccc
     7861 gatggatgac gcggaggccg acgcgtcagg gatgatgcgc caccaccgtt gcaatgctgg
     7921 gtggccgctg ggtgcggcga cccagatacc cgagttggaa cgcgtgatat cgtacggccg
     7981 gaacacgccg gatgggggcg tgggaagctc gctggcctgg tcgtcatcgt atgtaaccaa
     8041 ttgggccgac ccggcgtcgg acattgccaa actttccagg gcgccttctg tcataggaag
     8101 caggacgcca tcgattcgcg ccgggagggg tttgatcgac actttggctc gcaatacgaa
     8161 tttggccagg cgggcggcga aggccgccgc gatgtcttgt ttcagcagga tgaagaaagc
     8221 cgatggcggg tccgcccctt gccatacaat catgctggcc atgagacgcc cttttgggga
     8281 gcagtagccc gccagttgcg cgcgagcggg attggacgac gcgatgtcct gggtgagttg
     8341 tccttgcagg aacgtggcgg catcgggccc ggagacttca agcagggcga gatcgtcaag
     8401 gcttgcttcg agtggcgcgt gggaattcat ggatcctgta cttctctgat acgactgcga
     8461 caatggatac ggaaaaaagt agagtttatg ataacgggct tcagcctggt tgtgtggtgc
     8521 gcggggcggg gcgctctcag gcatgaggcg catgaaggcg gtcctcggat atggcgtgct
     8581 tgcggccatc atcgtagtgg cggcggcggc cggattcgcc tggcattggg tgtattcccc
     8641 ggtgaggatg gatgcgaacc ccatcgacta cacagtcgaa ccaggcagtt cgccgcgcat
     8701 catcgcccgg ctgatgaaca agcagggcat ccatgtgaac gaaacgggct tcgtcctgct
     8761 tgcgcggctc agcgggcgtg ccaaaggctt gaaggcgggc gcctacgaaa cccaggcggg
     8821 cgacaccccc tggaagctgc tcgaacgcat ggccaacggt gacatgaccc aggcccggct
     8881 gacgcttgtg gaaggttgga cctatcagcg catacgccag gccttgcggg atgaccccag
     8941 tgtcaagcag acgttgcagg gggtatccga caaagagttg ttgtcacgtc tagggtctga
     9001 caagaccaat ccggagggcc tcttcttccc ggacacgtat gttttcgcgc ccggcaccag
     9061 cgatttcgac attctgcgcc gggcctaccg cgctgaacag agcttgttgg ccacgctttg
     9121 ggctgatcgt gagccaggcc tgccgctgac gagtccttac caagcgctca ttctcgcgtc
     9181 gctcatcgag aaagagaccg gccatagcgc cgaccgacgc cgcgtcgccg gggttttcgt
     9241 caatcgtctg cgcctgaaca tgccattgca aactgaccca tcggtcatct atggcatggg
     9301 cgacgcatac catggcaaac tgcacaagaa ggatctggaa accgacacgc cgtggaacac
     9361 ctatacgcgc accgggttgc cgcccacgcc catcgcgtgc gtgggcaagg cgtccctgct
     9421 ggcggcgctg caccccgagg cccacaagta tctgtatttc gtgtccagag gggacggaac
     9481 gagcgagttt tccgaagacc tggcgagcca caaccgggcc gttgccaaat acattctggg
     9541 gaaacactag tgacgcaaga acggcggggc tgtctcatca cattggaagg gctcgatggc
     9601 gcgggcaaga gcacacacgt ccagtggttg gtcgactgtc tgcgttccaa agggatgtcg
     9661 gtgttgtcca cgcgcgagcc tggtggcacg gcgcttggcg agcaattgcg gcaaatggtg
     9721 ctgacccagc ccatgcggct cgaaaccgag acgctgctga tgtttgcggc acggtgcgag
     9781 cacatccata cggtcatcga accagcattg gccgatgggc gctgggtggt gtgtgaccgc
     9841 ttcaccgacg ccacctatgc ctatcagggt ggcggccgag ggttgggcgg cgcggcggtg
     9901 cgagcgctgg aacaatgggt gcaccccggt ttgcagccgg atctcacctt gttgttcgac
     9961 gtgcccctcg aggtcgcgcg ccagcgcctg gcgcggggcc gggatctgga ccggttcgaa
    10021 caggagggcg cgggtttctt cgaacgcacg cgcaatgcct atcacgaaag ggcgaccgca
    10081 caccctggcc gggtccgcat catcgattcc acactcggcg tcgagcaggt gcgcgagtcc
    10141 ctggctcggc agttggacat cttgttcgac aagcggcggg cttcgtcgac ggggcgctcg
    10201 gaatcatgac ccaaccaccg cgattccttc cgtggcagtt cgacatggcg cgtacatggc
    10261 tgggtgcgcg ggaacggttc gcccacgcct ggctcgtaca cggcctggcc ggcaccggca
    10321 agcgccagtt cgccctggcg gcggccgcca gcctgttgtg cgaacagccg gaacacggcc
    10381 tggcgtgcgg acactgcacg gcatgtcagt ggtttgccag tggcaaccat cccgacttcc
    10441 gtcgcatccg tccggatgcc gtcgcgctcg aagaaggcgc cgagcaggac gacactgagg
    10501 cgactggtgc cggcgcgagt aaaacaccct cagcggaaat ccgggtcgat cagctccgat
    10561 ccctgggcac ctggttcaac accgcgacac accgtggggg gtggcgcgtc gcggccttgt
    10621 atcccgccca tgcgctgaac acgatttccg ccaacgcgtt gctcaaggtc ttggaagagc
    10681 cgccagaaca cacggttttc ttgattgtgg cggatgcgcc ggatcgcctg ttgcccacgc
    10741 tggtgtcgcg ctgcaggcgg ttgcccgttc ccgtgccccc caggcaagac agtatcgcct
    10801 ggctgaaatc cgagggcgtg gcggatccag gacagtggct tgccagcgcc ggcggcgccc
    10861 cgttgctggc gctacgcagc gcgcgcgccg ccgacacgcc ctgccccgat tggctgtctg
    10921 ggtttctgat ggccttggct gaaggtggcg tggcggacgt cggtccgttg gccgatctgc
    10981 tggacaagac gccgccggaa gaatggctgg acgggcttca acgcttgttc atggatctgg
    11041 ccctcgcagg ggccagcgtg ccgctgcgct attatccaac cctggagggc ccgatccgca
    11101 tcgttgctga acgtgcatcg ttggctcgcc tcacccaggc cgggcactgg ctggccaagc
    11161 agcgccgctt tgcgcgccac ccgttgaatg cccggctgtt ggttcacgcc accctcgatc
    11221 ggcttgccgt ggcgtgccgg cccgtttgat gcgcgctggc ctgcgcttgc gggccggccg
    11281 ctgattttga aaagctattg ccatgttcgt tgattcccat tgccatctca attttcccga
    11341 tctcgccgcc aacctgccgg acatcctggc gcgcatggcg cgcaatcagg tcggccatgc
    11401 cctggtcgtc agcgtgaacc tgccggactg gcctggcctg atggcgcttg tggggcctca
    11461 cgccaatctt tatgcttccg tcggagttca tcccgattac gaagacacgc cggaaccgtc
    11521 ggtggacgaa ctggtcggcc tggcggccca acccaaagtg gtcgccatcg gagaaaccgg
    11581 cctggattac ttccggcttg ccgagccgct ggaatggcag cgcgagcgct ttcgcacaca
    11641 tatccgggcg agccgggcga gccggttgcc gctgatcatc catacccggg tcgcgagcgc
    11701 cgatacgctg cgcatcatgc aggaagaggg ggccgacgac atcggcggcg tcatgcattg
    11761 cttcacggaa tcatgggagg tggccgaggc ggccatggcg cagaacttct atatttcact
    11821 gtccggcatt gtcacgttca agagtgccaa ggcattgcag gacgtggcca cccgcgtgcc
    11881 gctggatcgc ctgttgatcg agacggattc gccctatctt gcgccagtac cgcatcgagg
    11941 tagtgtcaat gacccgtcaa aggtgattca cgtggccgag aagatcgccg agttgcgcgg
    12001 cgtgccggtg gcaaccatcg cggaacattc aactaataat ttcttcaaat tattcaataa
    12061 gataaatagg aatattgaag taaaacaata gaaaaatcga agtgtaatag ctcatatcgc
    12121 ttctgattcc aggcaagcca ttgtcggttt tgattctagc atttttgccc gtattaattt
    12181 aaagaaaaac attaatgaaa tttaataatt cattgataaa tataaaaaaa atcatatttt
    12241 tttcctgcat cttatggatt ggcgcgatgg cctccgccca cgctgccgtg ccggatggct
    12301 ggtggacgga tatcgcgaac aaccgggttt ccgacattga ggggatgctt ctgcaaggcg
    12361 ccaacccgaa tgcgatcgac ccgcatggcg acccgtccat catgttcgcc gtgcggcgca
    12421 aggcctggga tgtgttcgat gccctggcgg ccgacccgca gaccaatgtg aaccttgcca
    12481 atcgccttga tgaaacgccg ctgatgtatc tggcgatcgt ggggcagacc gcccgcgcac
    12541 ggacgctgat ccagcgtggc gccaaagtca accgtctggg ttggaccccg
//
LOCUS       DASTNK010000065        13327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_970923, whole genome shotgun sequence.
ACCESSION   DASTNK010000065 DASTNK010000000
VERSION     DASTNK010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13327
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970923"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VFR20_05150"
     CDS             <1..526
                     /locus_tag="VFR20_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0229937.1"
                     /translation="SGVDLAIRFGAVPDRRLYARRIMSNRRFLCASPLYLKRHGTPTS
                     LADLSDHQCILHRQNEDAYGVWRLSHNDRIEVVKVNGMLSSNDGDIVLSWALDGHGIL
                     LRSEWDLAKYVKSGRLCVVLPEYVQTSADLFIHYSYKRDQPARARAFIDFLVEHFKMD
                     NPPTAARNEPGFHV"
     gene            complement(607..4071)
                     /locus_tag="VFR20_05155"
     CDS             complement(607..4071)
                     /locus_tag="VFR20_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEU0229938.1"
                     /translation="MPLRMPGLKLSRRACKLFVGIVLTLFVLAVLATWLVPKVTRSLL
                     THNVSEMLGRQVQVGEIRFNPFSLALRARDISISKPQSAPLLQVGEVDLRVAWRSLVM
                     FAPVIDRLHIEKPRLSLVRTDATHFNFSDIVERLVKMSADKPGKPASTSTAPPRFSLN
                     NLTLANGTIGLDDRVTGRKQTIDDLTLGIPFISSFGYATHIDVLPAVHMRINGSPFSL
                     AGKALPFDTVPTSTLNVTLNGLALGKWADFWPMPLPIKIKSALLDSRLQVLFEQPSGA
                     APRIRIQGSLGVRKLDVVESSGAPLLAWDSLRVEHIAADLTARTVSIGTLTLEKPRVE
                     VHRDARQLNWQRVANGFAKLGGTTSPSADSAHATATPPGPASDASAKADHTPLEASTT
                     PGTAGKTPLPQAKPVPSKAAQQAWKIAIDTVKLDAGHMHVRDDPLGLDYPVDGLSAKV
                     EHVTLPQAAGQPMQVRLNMDNSHDGSSLRVEAPVVLQPLSIHGQVQLNKLPLAPFATA
                     LRHVAPMTLQGGRLDIEGKVDVAASRVEAHDVKLGLQQLAVRDEAVKPAVDLSIGALA
                     LTADRLALDTKPTNFTIQANGIQKHGTLALKGALTVRPLSLKTKVDLTNFDAASLAPY
                     IASSLNATVRSVSLNARGDAEFAPAQGHKTMHADWRGAIQVDHLDLQDRINRAKFLSW
                     KRLSLSNMHISTAGPQLGLGLGDIVLDDFYGNILLNDKAHLNVMDLMVGKGKAAGSIT
                     QDTQTRRSTEHARRHSGTKPDISIHSVTLKRGRMLFNDHFVRPNYRAELSSINGTLSA
                     VSSTRPAPARVMITGRVYGTAPLSVSGTVQPFSRYLALNLKASAKGVDLPRFTTYSSK
                     YVGYAIERGKLTVDLQYQIKHRALQASNKVRLDQLTFGKRSNSPDALKLPVLLAVALL
                     KDANGNIDINLPISGSLDDPQFSMGGIIVQVIVNTLTKVVTAPFKFLASTFGGGSDLS
                     YVGFAPGSSTLDAKAKSRIATLVKALIDRPALKMDIIGRADPQTDEPGLRQAWLDQQL
                     RKAKAMASHGKQPGARDIKLTDADRAKYLEKAYDNAKIKNKPRNAFGFTKSLPPEQMK
                     ALLLAAAPLGGQSLNALAEARARAVYEQITASSQDLADRVFIVSPKLDAAAINDGGPT
                     TRVDFALH"
     gene            complement(4333..5721)
                     /locus_tag="VFR20_05160"
     CDS             complement(4333..5721)
                     /locus_tag="VFR20_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU0229939.1"
                     /translation="MGDSAQSERFKNLKNSVAGDIIFPGGDTYDQARQVWNAMIDKHP
                     AAIVQCANTQDVVRTVEFARDADLRLAVRGGGHNIAGSALCDGGIVADLSRMKAVMVD
                     PSKRHAAVEGGATLGDFDTATQAHGLAVPLGINSTTGVAGLTLGGGLGWLSRKHGMTV
                     DSLQSAHVVTASGAVLRASTDEHPDLFWAIRGGGGNFGVVTRFEFKLHAVGPELLCGL
                     IVYPLAQAGAVLRQYRDFIAQAPDELAVWFLMRKAPPLPFLPPAAHGAGIIALGLLYA
                     GPPEDGGHLIEPLRRFGTPLGEHVGVMPYVSWQKAFDPLLAPRARNYWKSHNLIELSD
                     DLFDTLVGYAETLPSPQSEIFVGALGGATCRPSPDAMAYAHRDMRFVLNVHSRWDAPD
                     DDARCVAWARDFFQATTPFAGTGVYVNFMTADETDRIRTAYGRNYDRLAEIKRHYDPG
                     NLFSTNQNIAPA"
     gene            complement(5861..6595)
                     /locus_tag="VFR20_05165"
     CDS             complement(5861..6595)
                     /locus_tag="VFR20_05165"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEU0229940.1"
                     /translation="MNHRASWDRLAAEYALGTLRGAARARFETMLSRYPELAAATARW
                     QDALGVLDSHDAPVQPPDRVWRAILSRLPPAQQTPQGTPSSQQHTMGVRGRARLFVDR
                     WGWRLASLTLAAGLLVSVLRPFHPQSSGVTPSPIAVLSSSQPTTPRQELVISLDATKK
                     KLIVTPLDLRQPEADHSLQLWLIAPGRKPASLGLIAAETTTVIDLHAQRLNQGIALAV
                     SIEPLGGSPTGQPTGAVLYAGKVGRT"
     gene            complement(6592..7146)
                     /locus_tag="VFR20_05170"
     CDS             complement(6592..7146)
                     /locus_tag="VFR20_05170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0229941.1"
                     /translation="MARLSDDDHRHLLTAISLGDRDAFKALYDSTSPRLYAAALRLLK
                     RPAWAEEVLQDCFVTVWNRASSYNTTGSSPMTWLTHIVRNRAIDWLRGTDAHLTELDD
                     TGMAGLADPDGSPLDNLLRNDASRRLGNCMGLLAAEQRQCLVLAYYHGLSHEQVAGTL
                     QRPLGTTKSWIRRGLKQLKECLGL"
     gene            complement(7210..7758)
                     /locus_tag="VFR20_05175"
     CDS             complement(7210..7758)
                     /locus_tag="VFR20_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEU0229942.1"
                     /translation="MKKITWSVALATTLVAGIASAATVDVGGAAMYPQKNIIQNAVNS
                     KDHTTLVAAVKAAGLVSTLEGKGPFTVFAPTNAAFDKLPAGTVQNLLKPENKQQLIQV
                     LTYHVVPGRYTKHDLEKLIRKDGGKAHLKTAEGGTLTFMMNGPQNIQIKDSSGSVADI
                     STYDVFQKNGIIDVVDKVLLPK"
     gene            complement(7985..9034)
                     /locus_tag="VFR20_05180"
     CDS             complement(7985..9034)
                     /locus_tag="VFR20_05180"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU0229943.1"
                     /translation="MNSQALKDAVQFSIDHETVWDRDVNGAWGVHRNDPPPWNRLLGP
                     VHSRGPVSGTIVVDGETLVSWGEPRRADLTFSIAKTYLSLLAGVAHDRGILPDVHEPV
                     RVRVPGIGFDEGHNRDVTWEHMLQQTSEWEGVCFGVPDQADHYRAVTFGKPPGGRKGD
                     PRPLQTPGTHWEYNDVRINQFSLALLHLFGRPLPEIFGEAIMQPVGTVNDWNWVGYDN
                     AWVQIGGRRVQSVPGGTHWGGGLSISAEDQARIGRMLLNDGRVGNRQVLSSEWIARMR
                     KPCAIAPYYGYLVWLNSDRKIFPSAPESSYFGIGAGSSFCWMEPERNMVVVVRWLNPD
                     HADGFFGRVLEAVDG"
     gene            complement(9031..9861)
                     /locus_tag="VFR20_05185"
     CDS             complement(9031..9861)
                     /locus_tag="VFR20_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0229944.1"
                     /translation="MTPETPMLEIAGVTRDYRVGGMLRGRRTVNVLRGVDVSVPEGET
                     LGLVGESGCGKSTLARLMLGIEPPSSGTVRIAGRPISDYGRLERARLIQPVFQDPYSS
                     LNPRMTVGATILAPLEVQSEGTARSRRARADELMAAVGLPAHLFDAFPGQMSGGQRQR
                     VAIARALAIRPKILVCDEPTSALDVSVQSQVLNLIASIKQRFNLTIVLISHNLAVINH
                     LAHHVAVMYAGKIVEYGPTDDIFLHARHRYTQTLLDAVLVPEPGQRLPDITSYSEETP
                     "
     gene            complement(9858..10883)
                     /locus_tag="VFR20_05190"
     CDS             complement(9858..10883)
                     /locus_tag="VFR20_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010121877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0229945.1"
                     /translation="MAHLLEIEDLNIDIATDQALLQPVLGIDLTVDRGQALAIVGESG
                     SGKSLTALSILGLLPRRASLRARRIEFAGRSLQGLSNREWRAIRGDRISMIFQDPMTA
                     LDPCYTIGAQMTEVLLQHRPLSAHDARARVLKLLDTARVPSPEERFSQYPHELSGGLR
                     QRVMIAMSLLCEPDLLIADEPTTALDVTIQARILKLLDDIRRASGVGLIIITHDIGVV
                     ACVADVIAVMYRGQIVEHGPAQRILANPLHPYTEGLLRSIPVPGRVARGAPLGFIPGI
                     VPPPVGVPQACAFAPRCPYAMDACRTAPVTLGRVDDTHSVRCLLPTDGSGRDPDVWNR
                     LQLAEAS"
     gene            complement(10886..11791)
                     /locus_tag="VFR20_05195"
     CDS             complement(10886..11791)
                     /locus_tag="VFR20_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0229946.1"
                     /translation="MTDTSLSPPATPTVGALSPRRIAVRRALRHGSFWIGGIALLLII
                     LIALFAPWLTPYDPAAQHLGQRLIAPVWSTGGSWTHPLGTDLLGRDYLARLLYGARIS
                     LLIGFSTILISGVFGTCLGVAAGYWGGWVDLAVNFILTIRLTLPVVLVALVVVAVVGN
                     SLTLLIFVIGGLLWDRFTIVTRSATQQLAQSEFVIAARCIGSSRTRLFLKEILPNLTG
                     QLIVVASIELAHAVLLEAALSFLGLGVQPPLTSWGLMVAEAKSQIFFRPWMIAVPGVA
                     LIVLILSINLLGDAIRDITAPEGRA"
     gene            complement(11834..12751)
                     /locus_tag="VFR20_05200"
     CDS             complement(11834..12751)
                     /locus_tag="VFR20_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0229947.1"
                     /translation="MLGFILKRLALSVLVVAVVSVVAFSLVYLSGDPAAAMAGEGATQ
                     ADVAAVRATYGFDRPLYAQYGSWLRNVMHGDFGRSYYLREPVSYVLEQHFPITAKLGI
                     SALLFALLLSVPLGVLAALRPNTLIDRIALAIAVAGQAMPSFLFGLGLIYVFGVKLGW
                     LPFAGASSWDHFILPAIALGYYATPVIMRLTRSGMMDALQSDHVRTARAYGLSTWRVV
                     IKHALRHAIMPVLALSAVQLGFMLGGSIIIETVFSMKGLGYLAWMSIQRSDLQVIQAI
                     LLVIATLYALLTLLADLLNAFLDPRLRKR"
     gene            complement(12822..>13327)
                     /locus_tag="VFR20_05205"
     CDS             complement(12822..>13327)
                     /locus_tag="VFR20_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0229948.1"
                     /translation="DPALAKKLLAEAGYPNGFDQVIYAYRDRPYTEAVMNYLRAVGIR
                     TKLRFLQWKALRPLIDKGQVAMAQLTWGSQGMMDASASVSNYFEFSPDDYARDKQVRD
                     WLVAADHTTDPEARKTLYKKALTRIADQAYFIPLFTYARIYAFDSSLDYPVTPDELAH
                     FYLAHWK"
BASE COUNT         2377 a         3945 c         4501 g         2504 t
ORIGIN      
        1 aagtggcgtc gatttggcga tccgctttgg cgcggtgcca gacaggcggc tctatgcacg
       61 acgcatcatg tcgaatcgca ggttcctctg cgcctcgcca ctgtacctaa aacgacacgg
      121 caccccgacg agccttgctg atctttcaga tcatcaatgc atcctccatc gtcaaaatga
      181 agatgcctat ggggtttggc gtttgtcgca caacgaccgc attgaggtcg tcaaggtaaa
      241 cggcatgctg tcgagcaatg acggcgatat cgttctgagc tgggcgctcg atgggcacgg
      301 catactactc cgatcagaat gggatctcgc aaaatatgtc aagagcggaa gactttgtgt
      361 cgttcttcca gaatatgtgc agacctctgc tgatctcttt atccactatt cgtataaacg
      421 agaccagccg gcgcgtgcgc gagcgttcat cgactttctg gttgagcact ttaaaatgga
      481 caatccgccc acagcagcgc gtaacgagcc aggcttccat gtctgagcgg tcgggtcgat
      541 ggggtggggc gagctccccg aagcagcgcc tttcttgctc gttcgagctg tacttcctac
      601 ccacgctcaa tgcagggcga aatccacgcg tgtcgtgggg ccaccatcat tgatggcggc
      661 tgcgtcgagc ttgggagata cgatgaatac acggtcggcc aagtcttgcg agcttgcagt
      721 gatctgctcg taaacggcgc gtgcgcgagc ctcggcaagg gcattcaagg actgcccgcc
      781 cagcggggcg gcggccaaca gcaaggcctt catttgctcg ggcggcaatg atttggtaaa
      841 gccgaaggcg ttgcgcggct tgtttttgat cttggcgttg tcgtaggctt tttccagata
      901 cttggcgcgg tcggcgtcgg tcagcttgat gtcgcgggca ccgggttgct ttccgtggct
      961 ggccatggct ttggccttgc gcaactgctg gtccaaccag gcctgacgca ggccgggctc
     1021 gtcggtttgc ggatcggcgc gaccgattat gtccatcttc agcgcgggtc gatctatcag
     1081 cgccttgacc agcgtcgcga tgcgggattt ggccttggca tcaagcgtcg agctgccagg
     1141 agcgaagccc acatacgaca ggtcgctgcc gccaccaaag gtggaagcca ggaacttgaa
     1201 aggcgcggtc accaccttgg tcagtgtgtt gacgatcacc tgcacgatga tgccgcccat
     1261 cgagaactgt ggatcgtcca gcgagcccga gatgggcagg ttgatgtcta tgtttccgtt
     1321 ggcatccttc agcagggcga cggccagcag caccggcagc ttgagcgcgt cggggctgtt
     1381 ggaccgcttg ccgaaggtga gctggtcgag ccgcacctta ttgctggcct gcagggcgcg
     1441 atgcttgatc tggtattgca gatcgaccgt caacttgccg cgctcgatgg cgtagccgac
     1501 atatttggat gagtacgtcg tgaagcgggg tagatccacg cctttggcgg aagccttgag
     1561 attcagcgcg agatagcggg agaagggttg cacggtgccc gatacggaca ggggggcggt
     1621 gccgtacacg cggccagtga tcatcaccct ggccggcgcc ggtctggtcg acgacacagc
     1681 tgagagcgtg ccgttgatgg acgacagctc ggcgcggtag ttggggcgca cgaaatggtc
     1741 gttgaacagc atgcggccgc gtttgagcgt gacgctgtgg atggatatat cgggtttggt
     1801 gccgctgtgc cggcgagcgt gttcggtgct tcgacgagtc tgggtgtctt gcgtgatgga
     1861 gccggcggcc ttgcctttgc ccaccatcag gtccatgacg ttcaggtgcg ccttgtcatt
     1921 gagcaggatg ttgccgtaga agtcgtccag cacgatgtcg cccaatccca gtccgagctg
     1981 gggccctgcc gtcgagatgt gcatgttgct caggctaagg cgtttccagg agaggaattt
     2041 tgccctgttg atacggtcct ggagatcgag atggtcgacc tgaatggcac cgcgccagtc
     2101 cgcatgcatg gtcttatggc cttgcgccgg tgcgaattcg gcgtcaccgc gcgcgttcag
     2161 gctcacactg cgcaccgtgg cgttcaggct ggacgcgatg tacggggcca gcgaagcggc
     2221 atcgaaattc gtcaggtcca ccttggtctt cagcgacagc ggccgcaccg tcagcgcccc
     2281 cttgagcgcc agtgtgccgt gcttctggat gccgttggct tgtatggtga agtttgtcgg
     2341 tttggtgtcc agggccagcc gatcggctgt tagtgccagc gcaccgatgg acaggtcgac
     2401 tgcgggcttg accgcttcgt cgcgcacggc gagctgctgc agccccagtt tcacgtcgtg
     2461 cgcttcgaca cgggatgccg ccacgtccac cttgccctcg atgtccagcc ggcccccttg
     2521 cagggtcatg ggcgcgacat ggcgcagggc cgtggcaaac ggtgccaacg gcagcttgtt
     2581 caactggacc tgcccgtgga tggaaagagg ttgtagaacg acgggggctt cgacgcgcag
     2641 tgagctgcca tcgtggctgt tatccatatt caaccgcact tgcatgggct gacccgcggc
     2701 ctgcggcagg gtgacgtgtt caaccttcgc gctcaggcca tccaccgggt agtccaggcc
     2761 gagtgggtcg tctcgcacat gcatgtggcc ggcatcgagc ttgacggtgt caatggcgat
     2821 tttccaggcc tgctgtgccg ctttgctagg cacgggttta gcctgcggca ggggtgtctt
     2881 gccggcggtg ccgggtgtgg tgcttgcttc gagtggggtg tgatcagcct ttgccgaggc
     2941 gtcggacgcg ggaccaggag gcgtggcggt ggcgtgcgcg gaatcggcag acggggatgt
     3001 agtgccaccc agcttggcga agccgttggc cacacgctgc cagttcaact gcctggcatc
     3061 gcggtgtacc tctactcgcg gcttctccag ggtcaacgtg ccgatggaaa ccgtgcgcgc
     3121 ggtgaggtcg gccgcgatgt gttcgacacg caggctatcc caggccagca gcggggcgcc
     3181 cgaggactcg accacatcga gcttccgcac acccaggctg ccctgtatgc ggatgcgcgg
     3241 cgcagcgccc gagggttgtt cgaacagaac ctgcagcctg gagtcgagca gcgcgctctt
     3301 gattttgatg ggcagcggca tgggccagaa gtcggcccat ttgccgagcg ccaggccatt
     3361 cagcgtcacg ttcagcgtgg atgtcggcac ggtgtcgaag ggcagcgcct tgccggccag
     3421 gctgaagggg ctgccattga tgcgcatgtg cacggcgggc agcacgtcga tgtgcgtggc
     3481 gtagccgaac gaagaaatga aggggatgcc cagcgtgagg tcgtcgatcg tctgcttgcg
     3541 gcccgttacg cggtcgtcca ggccgatggt gccgttggcc agcgtcaggt tgttcagcga
     3601 gaaacgtggt ggcgctgtgg aggttgacgc cggcttgccc ggtttgtcgg ccgacatctt
     3661 gaccagccgc tcgacaatat cggagaaatt gaaatgcgtg gcgtcggtcc ggaccagaga
     3721 gagcctgggc ttctcgatgt gaagccggtc aatgaccggc gcgaacatga cgagtgatcg
     3781 ccaggccacg cgcaggtcga cttccccgac ctgaagcagc ggtgcggact ggggcttgct
     3841 gatcgagatg tcccgcgcgc gcaacgccag cgagaagggg ttgaagcgga tctcccccac
     3901 ttgcacctgg cgacccagca tctcggagac gttgtgggtg agcaggctgc gcgtcacctt
     3961 gggaaccagc caggttgcaa gaacggccag gacgaacagt gtgagcacga tacccacgaa
     4021 aagcttgcat gcccgtcgac tgagtttcaa acctggcatc cgcagcggca tcgcgcctct
     4081 tcccctctgg attgttgtgt cccgtatccg gtctgcgggc attggcggtc cgcagcgcgt
     4141 ggctcgacca attatccctg aggggatccc gctcgtccat caaagagagg ggaactttac
     4201 tattgctgtc ttgtgatggt gtaactatcc ttgtcgggct ggcgcgatgt cttggttgtt
     4261 ggatccgagc agaacttctc tatggtgcgc agaccctgcc agatgggctg cactcgccac
     4321 gcgccgtctc gttcaggccg gcgctatgtt ctggtttgtt gaaaacaggt tgccgggatc
     4381 gtagtgccgt ttgatttccg ccaagcggtc ataattcctt ccataggccg tgcgtatgcg
     4441 atcggtttcg tcggcggtca tgaagttgac gtaaacaccg gtgccggcga acggcgttgt
     4501 cgcctggaag aagtcgcgcg cccaggccac gcagcgcgca tcatcgtcgg gcgcgtccca
     4561 gcggctatgc acattgagga cgaagcgcat gtcgcggtgc gcataagcca ttgcgtctgg
     4621 cgaagggcgg caggtcgcgc cacccagggc gcccacgaag atctcgctct ggggcgaggg
     4681 aagggtctcg gcgtaaccga ccagcgtgtc gaacaaatcg tcgctcagtt cgatgaggtt
     4741 atgcgatttc caataattgc gcgcgcgcgg cgccagaagt gggtcgaagg ctttctgcca
     4801 cgaaacgtat ggcatgacgc ccacgtgttc gcccaaaggg gtgccgaaac gacgcaacgg
     4861 ctcgatgaga tgccccccgt cttcaggcgg gccggcgtac agcaacccca gggcgatgat
     4921 gcctgctccg tgtgcggcgg ggggcaggaa gggcaggggc ggcgccttgc gcatcaggaa
     4981 ccagacggcc agctcgtccg gcgcttgtgc gatgaaatcg cggtattgtc gcagtaccgc
     5041 gccagcctgc gcgagcgggt agacgatcag gccgcagagc agttcggggc ccaccgcatg
     5101 gagtttgaat tcgaaacggg tcacgacgcc gaaattgccg ccccctccgc gtattgccca
     5161 gaacaggtcg ggatgttcgt cggtgctggc gcgcaggacc gcgccgctgg cggtcacgac
     5221 gtgcgcggat tgcaggctgt ccacggtcat gccgtgcttg cggctgagcc agcccaggcc
     5281 cccgcccaga gtcagcccgg cgacaccggt ggtggagttg atgcccaggg ggacggcgag
     5341 gccgtgggcc tgggtggccg tgtcgaagtc gcccagtgtg gcgccgcctt cgaccgcggc
     5401 gtggcgttta ctcggatcga ccatgacggc tttcatgcgt gacaggtcgg cgacgatgcc
     5461 gccatcgcac agcgcgctac ccgcgatgtt gtgccccccg ccacgcacgg ccaaccgcag
     5521 gtccgcgtcc cgggcgaact cgactgttcg caccacgtct tgcgtgttgg cgcattggac
     5581 gatggcggcc ggatgcttgt ctatcatggc gttccatacc tggcgggcct ggtcgtaggt
     5641 atcgccgccc ggaaatatga tgtcgcccgc cacgctgttt ttcagattct tgaaccgctc
     5701 cgattgtgca gagtcgccca tgacggcctc ctatcgcgcg tgttgattgt gcccactata
     5761 cgctcggtgc ccacaacatg ggggggtgaa aaatgtgctt acacggcgcg gggcgggggt
     5821 gtgcttgtgg cgcgaaagcc tgctcgaggt ggcggcgaaa tcaggtccgc cccaccttcc
     5881 ccgcgtacag gacggccccg gtgggttgcc cggtgggtga tccgcccaac ggctcgatgc
     5941 tgaccgccag ggcgataccc tgattcaagc gttgggcatg caggtcgatg actgtcgtgg
     6001 tttctgcggc gatcaagccc agggacgcgg gttttcggcc tggggctatc agccacagtt
     6061 gcaggctatg gtcggcttcg ggctggcgca gatcaagcgg tgtgacgatg agtttcttct
     6121 ttgtggcgtc caggctgatg accagttcct gccgtggcgt cgtgggctgt gacgagctca
     6181 ggacagcgat gggcgatggc gtcacgccgc tggattgcgg atggaatggc cgcagcactg
     6241 aaaccagcaa cccggccgcc agcgtgaggc tggccaaccg ccagccccag cgatcgacga
     6301 aaaggcgtgc gcgcccgcgc acacccatgg tgtgttgttg ggatgatggc gtgccctgtg
     6361 gagtttgctg ggcgggcggc agccgcgata gtatggcgcg ccacacccga tccggtggct
     6421 gcaccggtgc gtcgtgcgaa tccagcacac cgagggcgtc ttgccagcgt gccgtcgcgg
     6481 cggcgagttc cggatagcgg gaaagcatgg tttcgaagcg cgcgcgcgcg gcgccgcgca
     6541 aggtgcccag cgcgtattca gccgccagtc tgtcccagga cgcgcgatgg ttcataagcc
     6601 cagacactct ttcagttgtt tcagcccgcg ccgtatccag cttttcgtgg tccccagggg
     6661 ccgttgcaat gtaccggcaa cctgttcgtg tgaaaggccg tgatagtacg ccagcaccag
     6721 gcactgccgc tgttcagcgg cgagcaggcc catgcagttt cccaggcgcc gcgaggcatc
     6781 gttccgcagc aggttgtcca gtggggagcc gtcagggtcg gccaggccgg ccatccctgt
     6841 atcgtcgagt tctgtcaggt gcgcatcggt gccgcgcagc cagtctatgg cgcggttgcg
     6901 gacgatgtgc gtgagccagg tcatgggtga gctgccggtg gtgttgtagc tgctcgccct
     6961 gttccagact gtcacgaagc agtcttgcag cacttcctcg gcccaggcgg gacgcttgag
     7021 caggcgcaag gccgccgcgt acaagcgcgg cgacgtgctg tcgtacaacg ccttgaaagc
     7081 gtcccggtcg cccagggata tggcggtcag tagatgtcgg tgatcgtcgt cggagagacg
     7141 tgccatgcac ttccttgggt gctggccggc ccacaaaggg ccggatggag gaaattgcgc
     7201 gacacggggc tacttgggaa gcaggacctt gtccacgaca tcaatgatac cgttcttctg
     7261 gaagacgtca tacgtgctga tgtcggccac actgccagac gagtccttga tctggatatt
     7321 ctgcggaccg ttcatcatga aggtcaacgt accaccctcg gctgtcttga gatgcgcctt
     7381 gccgccgtcc ttccgtatca gtttttccag atcgtgcttc gtgtatcggc ctggcaccac
     7441 atggtaggtc aatacctgga tcaactgctg tttgttttcc ggtttcaata gattctggac
     7501 ggttccggcg gggagtttgt cgaacgcggc gttggtgggg gcgaacaccg tgaagggccc
     7561 tttgccttcc agggtggaaa ccagcccggc ggctttgacg gcggcaacca gggtggtgtg
     7621 gtccttggaa ttcacggcgt tttgaatgat gttcttctgg gggtacatcg ctgcgccgcc
     7681 cacgtcgacc gtggcggcgc ttgcgatgcc agcgaccagc gtagtcgcca gagcgactga
     7741 ccaggtgatt tttttcatga tgtttccttc ggtgatgaaa gtgtttgtcg agtaccacac
     7801 tgacatatac gaaggaatgg ccggtttgga tgcacccaat caaaaataat ttttgatatc
     7861 aaagacgggg atgtaaacag aaacaaacgg cttgggtccc agctccggat ggccgacgcg
     7921 caaagcgatg cgagtcgccg tcgagcacgc cggtggtgca aggtgctgaa tcgccccgct
     7981 tttctcagcc gtccacggcc tccagcaccc ggccgaagaa cccatcggca tggtcggggt
     8041 tcagccatcg caccacgacc accatgttgc gctcgggctc catccagcag aaggaactgc
     8101 ccgcgccgat gccgaaatag ctggattccg gcgcgctggg gaaaatcttg cggtcactat
     8161 tcaaccagac cagatagccg taatagggtg cgatggcaca gggtttgcgc atgcgcgcga
     8221 tccattccga ggacagcacc tgtcggttgc cgacgcggcc gtcgttcagc agcatccggc
     8281 cgatacgcgc ctgatcctcg gcgctgatgg atagcccgcc accccaatgg gtgccaccgg
     8341 gcacggattg cacgcggcgc ccgcctatct gaacccaggc gttgtcgtag cccacccagt
     8401 tccagtcatt gaccgttccc acgggttgca tgatggcctc cccgaagatc tccggcagcg
     8461 ggcggccaaa caagtgcagc aaggccagtg aaaactggtt gatgcgcacg tcgttgtatt
     8521 cccaatgggt tccaggtgtc tgcagggggc gggggtcgcc cttcctgccc ccaggaggct
     8581 tgccgaacgt gaccgcccga tagtggtcgg cctgatcggg tacgccgaaa cacacgcctt
     8641 cccattcgct ggtctgctgc agcatgtgtt cccaggtgac atcgcggttg tggccctcgt
     8701 cgaagccgat gccgggcacg cgcacacgaa cgggttcgtg cacatcgggc aggatgccgc
     8761 gatcgtgcgc cacgcccgcc agcagcgaca gataggtttt cgcgatgctg aacgtcaagt
     8821 cggcgcgccg gggctcgccc caggaaacca gggtttcgcc gtccacgacg atggttcccg
     8881 acaccgggcc gcgtgaatgc acgggaccca gcaggcggtt ccacggtggc gggtcgttcc
     8941 ggtgcacgcc ccaggcgcca ttgacatccc ggtcccacac ggtttcatga tcaatggaga
     9001 actggacggc atccttcaat gcttgagaat tcatggtgtt tcctcggaat agctggtgat
     9061 atccggcagg cgctgtccgg gctctggcac cagcacggcg tccagcagcg tttgcgtata
     9121 gcggtgacgg gcatgaagaa aaatgtcatc ggtcgggccg tactcgacga ttttcccggc
     9181 gtacatcacc gcgacatgat gcgccagatg attgatcacc gcgaggttgt ggctgatgag
     9241 cacaatggtc aggttgaagc gttgcttgat cgaggcgatc agattcaaca cctgggactg
     9301 caccgatacg tccagggccg acgtgggttc gtcgcagacg aggatcttgg ggcgtatggc
     9361 cagggcccgc gcgatcgcga cgcgttggcg ctgaccgcca gacatctggc ccggaaacgc
     9421 gtcgaacaag tgcgcgggaa ggcccacggc ggccatcaat tcatcggcgc gcgcacgacg
     9481 ggagcgcgcc gtgccttcgg actgcacttc gagcggtgcg agaatggttg ccccgacggt
     9541 catgcgcgga ttcagggaag aataggggtc ttgaaacacc ggctgtatca gtcgtgcccg
     9601 ctccagccgg ccatagtcgg agatggggcg tccggcaatc cgcaccgtgc cactggaggg
     9661 gggctcgatg ccgagcatca ggcgcgccag cgtgcttttg ccgcatcccg attcaccgac
     9721 cagacccagt gtttcgccct ctgggaccga gacgtctacg ccacgcagca cgttcacggt
     9781 gcgccggccg cgcagcatgc cgccgacccg gtagtcccgc gtgacgccgg cgatttcgag
     9841 catgggcgtc tcgggggtca tgatgcctcc gccagttgca ggcggttcca tacgtcgggg
     9901 tcgcgaccgc tcccgtccgt tggcagcagg cagcgcacgc tgtgagtgtc gtccacgcga
     9961 cccagtgtca cgggcgccgt tcgacacgcg tccatggcgt aggggcagcg cggggcgaag
    10021 gcgcaggctt gtggcacgcc cacggggggc ggcacgatgc cggggatgaa gcccaggggc
    10081 gccccccggg ccacgcggcc ggggacgggg atcgagcgca acagcccctc ggtgtagggg
    10141 tgcaaggggt tggccagaat gcgctgggcg gggccgtgct cgacgatctg gccccggtac
    10201 atgacggcga tgacatcggc gacgcaggcg accacgccaa tgtcgtgcgt gatgatgatg
    10261 aggcccacgc ccgacgcgcg gcggatgtca tccagcagtt tcagtatccg ggcctggatg
    10321 gtgacatcca gcgcggtggt gggttcgtcg gcaatgagca gatcgggttc gcacaacagc
    10381 gacatggcga tcatcacacg ctggcgcagg ccgcccgaca actcgtgcgg gtattggctg
    10441 aaacgctctt cgggagacgg cacccgcgcg gtgtccagca gcttcagcac gcgcgcccgg
    10501 gcatcgtgcg cgctcagggg gcgatgctgc aaaaggactt ccgtcatttg cgcgccgatg
    10561 gtgtagcagg gatccagcgc ggtcatgggg tcctggaaga tcatcgagat ccgatcgccg
    10621 cggatggccc gccattcgcg attgctcagg ccttgcagag accgtccggc gaactcgatg
    10681 cgccgggcgc gcaggctggc gcggcgcggc agcaacccca gaatggacag ggccgtcagg
    10741 ctcttgccgc tgccggattc tccgactatg gccagggcct ggccacgatc gacggtcagg
    10801 tcgatgccga gcacgggctg aagtaacgcc tggtcggtgg cgatgtcgat gttcagatct
    10861 tcgatttcca gcagatgcgc catcgtcagg ccctcccttc gggcgcggtg atatcgcgta
    10921 tcgcgtcgcc cagcaggttg atggacagga tcaacacgat cagcgccacg cccggcacgg
    10981 cgatcatcca gggtcggaaa aatatctggc ttttggcttc agccaccatc aggccccagg
    11041 acgtcaaagg gggttgcaca cccagcccca ggaacgacag cgcggcctcg agcaggacgg
    11101 cgtgggcgag ctctatggat gcgaccacga tgagctggcc ggtcaggttg gggagtattt
    11161 ccttcaggaa cagccgtgtg cgggaactgc ctatgcagcg ggccgcgatc acgaactcgg
    11221 attgggcgag ttgctgtgtg gcgctgcgcg tgacgatggt gaaccggtcc cacaacaggc
    11281 cgccgatgac gaagatcagc agcgtcaggg agttgccgac cacggcgacc acgaccagag
    11341 cgacgagcac cacggggagt gtcaggcgta tggtcaggat gaagttgacc gccagatcga
    11401 cccagccgcc ccagtagcct gccgccaccc cgaggcaggt gccgaaaacg ccggagatca
    11461 ggatggtgga aaagccgatg agcagcgaga tgcgggcgcc atacagtagc cgcgccaagt
    11521 agtcccggcc gagcaggtcg gtgcccagag ggtgcgtcca actgccaccg gtggaccata
    11581 ccggtgcgat caggcgttgg ccgaggtgct gggccgccgg gtcgtagggg gtcagccatg
    11641 gcgcgaacag ggcgatcagg atgatcagca gaagcgcgat gccgccgatc cagaagctgc
    11701 cgtgtcgcag tgcccggcgc acggcgatgc ggcggggcga cagggcgccg acggtcggcg
    11761 tggcgggtgg cgataacgag gtgtccgtca tggtggctag ggcttgttca tgctgaatgg
    11821 cgtcccatgt ggttcaccgc ttgcgcaggc ggggatcgag gaaggcgttg agcagatcgg
    11881 ccaacagggt cagcaacgcg tagagcgtcg cgatcaccag caggatggcc tggatgactt
    11941 gcaggtcgga gcgctggatc gacatccagg ccaggtaccc caggcctttc atggaaaaca
    12001 cggtttcgat gatgatggag ccgcccaaca tgaacccgag ctgtaccgcg ctgagcgcca
    12061 gcaccggcat gatggcgtgg cgcaaggcat gcttgatcac tacgcgccat gtcgacaggc
    12121 cataggctct tgccgtgcgc acatggtcgg attgcaacgc atccatcatg ccggagcgcg
    12181 tcaggcgcat gatgaccggc gtggcgtaat agcccagtgc gatcgccggc aggatgaagt
    12241 gatcccacga cgacgcgccg gcgaatggca gccaccccag cttcacgccg aagacgtaga
    12301 tcaggcccag cccgaacaga aaggacggca tggcctgccc cgcgacggcg atggccagcg
    12361 ctatccggtc tatcagggtg ttggggcgca gggccgcgag cacgccaagc ggcaccgaaa
    12421 gcagcaacgc gaacaacaag gcgctgatgc ccagcttcgc cgtgatggga aaatgctgct
    12481 ccaggacgta ggagacgggc tcccgcagat agtacgaccg cccgaagtcg ccatgcatga
    12541 cgttgcgcag ccaggatccg tactgcgcgt atagcggacg gtcgaaccca taggtggcgc
    12601 gcacggccgc cacgtcggcc tgcgtcgcgc cctcgccggc catggcggcg gcggggtcgc
    12661 ccgacagata caccaacgag aaggcgacga ccgacaccac ggccacgacc aggacgctca
    12721 gggcgaggcg cttgagaatg aaccctaaca tggttggatg cgctgtggtg ggcaccgaaa
    12781 gcgcgccatg cctggcgcgc ccatgaaagg ccagcggagg atcatttcca gtgcgccaga
    12841 tagaagtgcg ccagttcgtc cggggtcacc gggtagtcca ggctggagtc gaaggcataa
    12901 atgcgggcgt acgtgaacag ggggatgaaa taggcctggt cggcgatacg ggtcagcgcc
    12961 ttcttgtaca gcgttttgcg cgcttcgggg tcggtcgtgt ggtcagccgc gaccagccag
    13021 tcgcgcacct gcttgtcgcg cgcatagtcg tcggggctga actcgaagta attgctgacc
    13081 gacgccgacg cgtccatcat gccttgtgaa ccccaggtca gttgcgccat ggccacctgg
    13141 cctttgtcga tgagcggccg cagggccttc cattgcagga accggagctt ggtgcggatg
    13201 ccgaccgcgc gcagatagtt catgacggct tccgtgtagg ggcggtcgcg gtaggcgtag
    13261 atgacttggt cgaagccgtt ggggtagccg gcctcggcca gcagcttctt ggccagcgcc
    13321 gggtcgt
//
LOCUS       DASTNK010000066         4509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_419080, whole genome shotgun sequence.
ACCESSION   DASTNK010000066 DASTNK010000000
VERSION     DASTNK010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419080"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..893
                     /gene="yghU"
                     /locus_tag="VFR20_05210"
     CDS             15..893
                     /gene="yghU"
                     /locus_tag="VFR20_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEU0229949.1"
                     /translation="MADSQEYVPPPVWVWNKPNGGHFQSINRPIAGPTHDKVLPVGRH
                     PLQLYSLATPNGQKVTIMLEELLALGHRGADYDAWLIRIGDGDQFGSGFVEVNPNSKI
                     PALMDRSGATPIRVFESGSILVYLAEKFGALLPTEQPARAECLSWLFWQMGSAPYLGG
                     GFGHFYAYAPVKIEYAIDRYAMEVKRQLDVLDRRLADNEYLGGGDYTIADIAVFPWYG
                     GLAKGWQYGAAEFLSVQDYKNVQRWADAILARPAVQRGRMVNRTSGDPAKQLRERHDA
                     SDFDTKTQDKLEAAQP"
     gene            992..2221
                     /locus_tag="VFR20_05215"
     CDS             992..2221
                     /locus_tag="VFR20_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEU0229950.1"
                     /translation="MITVDEKRLWDSLMDMAAVGRTANGGSCRLTLTEDDVAGRRLFM
                     RWCEEAGCQVYTDEVGNIFARRAGTDAGAAPVMCGSHLDTQPLGGRFDGVYGVLAGLE
                     ALRTFNTMGIVTRHPVEVAAWTNEEGSRFTPGILGSSVYAGRADLAFARARPCMTTGV
                     TLGEELDRTGFSNPPRFRDRPVAYYEAHIEQGPVLEQAALPLGVVTGVQGLYEYDVTL
                     TGFESHAGTTPMNVRRDALACAAGIIATIADLGRRSDDDTRLTVGHIACRPNSPSTIP
                     GSVMFSIDIRHPDIHRLDELADRVAAICRERCQAENVALDCARVAAYEPIQFNPACVD
                     RVRQASAELGFPSMDMCSGAGHDAVNLSYVAPSAMIFVPCRNGLSHNEAEYAEPHHLA
                     QGASVLANVLLAEAGRG"
     gene            complement(2124..3869)
                     /locus_tag="VFR20_05220"
     CDS             complement(2124..3869)
                     /locus_tag="VFR20_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU0229951.1"
                     /translation="MRKISLIPRLSVRTSLILALVFFEALLIAGAALGLWSLRTDRAA
                     LTQISSDQEASRALGEAVVQYKDIQAALGKALANDAAVHQAAAAKAAGLAATGAHIDK
                     SKTQTYLDAAKQALNTSRDNFASYEKMVKGHGNPAYAKVKTTYEALMGKGVGPLFDFA
                     SKGDITGYAGYLSSTTQGLEDDFSQALGFARATQWRTTNTIKTGQATNYQRITILVAA
                     AAGVSILFAILTYFMLGRVVLRPLRTVQSHFARIASGDLTQRVEVKTHNEIGVLFEAL
                     RNMQESLTSTVSAVRQGVEEITTGSREIFVGNTDLSSRTEQQAASLQQTAASMEELAS
                     TVKQNTDNAMQADELAKKASDVAQRGGDAVGQAVGSMDAISTSAGKISEIVSVIDGIA
                     FQTNILALNAAVEAARAGEQGKGFAVVAGEVRSLAQRSAQAAKEVKGLIEESLSRVQA
                     GSAQVRQAGDIMRDVVEAVSGVTAIMNEIASASREQSEGIDQVNQAVTQMDEVTQQNA
                     ALVEQAAAASGSLQEQAARLHKAVAVFILEREKKEGAEAPVISPARPLQEAHSPAPTP
                     PVPNGAAPHIQLRYG"
BASE COUNT          736 a         1376 c         1478 g          919 t
ORIGIN      
        1 caaggagttc gagcatggca gattcccaag agtacgtccc gccgccagtc tgggtctgga
       61 acaaacccaa cggtggtcat ttccagagca tcaaccggcc gatcgcgggc cccacgcacg
      121 acaaggtgct gccagtcggg cgccacccgc tgcagcttta ctccctggcc acgcccaacg
      181 gccagaaggt caccatcatg ctggaggaac tgctggcgct ggggcaccgc ggcgccgact
      241 atgacgcgtg gctgatacgc attggcgacg gcgaccagtt cggcagcggc ttcgttgagg
      301 tcaatccgaa ttccaagatt ccggccctga tggaccggtc cggcgcgaca ccgatccggg
      361 tgttcgagtc cggttcgatt ctggtctatt tggccgagaa attcggagcg ctgctgccca
      421 ccgagcagcc ggcccgtgcg gaatgccttt cctggttgtt ctggcagatg ggcagcgcgc
      481 cctatctggg gggcgggttc ggccactttt atgcctatgc accggtcaag atcgagtacg
      541 cgatcgatcg ctacgccatg gaggtcaaac gccagctcga tgtgctggat cgccgcctgg
      601 cggacaatga gtatctgggc ggtggcgatt acaccattgc cgacatcgcg gtgtttccct
      661 ggtacggcgg gttggccaag ggttggcaat atggagccgc cgagttcctg tccgtgcagg
      721 actacaagaa cgtgcagcgt tgggccgacg ccatcctggc caggcctgcg gtgcaacgcg
      781 gccgcatggt caaccgcacc tcgggtgacc cggccaagca attgcgtgaa cgccatgatg
      841 cttccgattt cgacacgaaa acacaagaca agctggaagc cgcgcagccg tgaatttcct
      901 gactcggata aagtggcgcc tgtgacggcg ttggcgccgg acgtcggcct gccaaaaggc
      961 gggcttcgac ggtcacctcg atggagataa cttgattact gtcgatgaaa agcggctttg
     1021 ggattcgttg atggacatgg cggccgtcgg gcgcacggcc aacggcggca gttgtcggtt
     1081 gaccctcacc gaagatgatg tggcggggcg gcgcctgttc atgcggtggt gtgaagaggc
     1141 cggctgccag gtgtataccg acgaggtggg gaacattttc gcccgccgtg ccggcaccga
     1201 tgccggtgcc gcgccggtga tgtgcggcag ccacctcgat acccagccgc ttggcggtcg
     1261 ctttgacgga gtctatggcg tgctggccgg gcttgaagcc ctgcgcacgt tcaacacaat
     1321 gggcatcgtc acccggcatc ccgtggaggt cgccgcctgg accaatgaag agggctcgcg
     1381 cttcacgccg ggcatactgg ggtcgtccgt gtatgcgggg cgggccgacc tggcgttcgc
     1441 gcgcgcccgc ccctgcatga cgacaggcgt gacactgggc gaggaactgg atcgcaccgg
     1501 cttttcgaac ccgccgcgct tccgtgatcg gccggtggcc tactacgagg cccacattga
     1561 acaggggccg gttctggagc aggcggcgtt gcccctgggg gtggtgacgg gagtgcaggg
     1621 cttgtatgaa tatgacgtga ccctcaccgg gttcgagtcc catgccggca ccacgcccat
     1681 gaacgttcgc cgggatgcgt tggcctgcgc cgctggcatc atcgcgacca tcgccgacct
     1741 ggggcggcgg tcggacgacg atacccgtct gacggtcggg catatcgcct gccggccgaa
     1801 ctcgcccagc accattccag gcagcgtgat gttcagcatc gacatcaggc accctgacat
     1861 ccaccggctg gacgaattgg cggatcgggt cgccgccatt tgtcgtgaac gatgccaggc
     1921 cgagaacgtg gcgctcgatt gtgcgcgcgt ggcggcctac gagcccatcc agttcaatcc
     1981 cgcctgcgtg gatcgagtgc gccaggccag cgccgagttg ggtttcccca gcatggacat
     2041 gtgcagcggc gccgggcacg acgccgtcaa tctgtcatat gtggcgccgt cggcgatgat
     2101 attcgtgccg tgccgcaacg gcctcagcca taacgaagct gaatatgcgg agccgcacca
     2161 tttggcacag ggggcgtcgg tgctggcgaa tgtgcttctt gcagaggccg ggcggggctg
     2221 atgacggggg cttccgcgcc ttcctttttt tcccgttcca ggatgaacac cgccacggct
     2281 ttgtgcagcc gcgcggcctg ttcttgcaaa gaacccgagg cggccgcggc ctgttcgacc
     2341 agtgcggcgt tctgctgagt gacctcgtcc atctgcgtga ccgcctggtt gacctggtct
     2401 atgccttcgg attgctcgcg cgacgccgag gcgatttcgt tcatgatggc agtgacgccc
     2461 gatacggcct cgacgacgtc gcgcatgatg tcacccgcct ggcgcacctg cgctgaaccc
     2521 gcctgcacac gcgacagcga ttcctcgatg aggcctttga cttccttggc tgcctgggcg
     2581 ctgcgttgcg cgagcgagcg gacttccccc gccaccacgg cgaagccttt gccctgttcg
     2641 ccggcgcgcg cggcctcgac ggccgcgttc aacgccagaa tattggtctg gaacgcgatg
     2701 ccgtcgatga cgctgacgat ttcggagatc ttgcccgcgc tggtggagat ggcgtccatg
     2761 gatcccaccg cctggcccac cgcgtcgccg ccgcgttgcg cgacgtcgga ggctttcttg
     2821 gccagttcat cggcttgcat ggcgttgtcg gtgttctgct tgaccgtgct ggcgagctcc
     2881 tccatgcttg ccgctgtctg ctgcagagac gccgcctgct gttcggtgcg ggagctgagg
     2941 tctgtgttgc cgacgaagat ttcgcgcgac cccgtggtga tttcctcgac accctggcgc
     3001 acggccgata cggtgctggt gaggctttcc tgcatgttgc gcagcgcttc gaacaacaca
     3061 ccgatttcgt tgtgggtctt cacctcgacg cgttgagtca ggtcgcccga ggcgatgcgg
     3121 gcgaaatggg actgcactgt gcgcagcgga cgcagcacga cccgccccag catgaaatag
     3181 gtcagaatcg cgaataggat ggacacgccc gcggcggcag ccaccagaat ggtgatgcgt
     3241 tgatagttcg ttgcctggcc ggtcttgatg gtattggtcg tgcgccactg tgtcgcacgc
     3301 gcgaaaccca gcgcttgcga gaagtcgtct tccaggcctt gcgtggtgct cgacagatat
     3361 ccggcgtagc cggtgatgtc gcccttggac gcgaagtcga acagtggccc gacgcccttg
     3421 cccatcaggg cctcgtaggt ggtcttgacc ttggcataag cgggattgcc gtggcctttg
     3481 accattttct cgtaagaggc gaagttgtca cgcgaggtgt tcagcgcctg cttggcggcg
     3541 tccaggtagg tttgcgtctt gcttttgtcg atgtgcgcgc cggtggcggc gagaccggcg
     3601 gctttggccg ccgcggcctg atggaccgcc gcgtcattgg ctagtgcctt gcccagcgcc
     3661 gcctgaatgt ctttgtattg cacgacggcc tcgcccagtg cgcggctggc ctcttggtcg
     3721 gacgagattt gcgtgagcgc ggcgcggtcc gtgcgcagcg accacaggcc cagcgcggcg
     3781 ccggcgatca acaacgcttc gaagaagacc agtgccagga tcaggctggt gcgcacgctc
     3841 agccgtggaa tcagggagat ttttcgcatg atgaggccct ttcctggaag aggtgggggt
     3901 ggcgcggccg gataggccgg ccggtgtcac agaaggccag cgctggctac gccagcccgt
     3961 aatgatgggt aaagtcatta acgactctac ctggccgcac ctttagcaca gccgtatcag
     4021 catcaaccct aacgcctcat caccctgcat gtggatccgt ttctgaagca cgatggttat
     4081 cagcgccggc aggtgggctg ggccggatgg tgggaattct cttcagcgga tccttgggcg
     4141 tccagccgtc acaatgggtg aaaaactgac gttattctga aaattgagcg attttggggg
     4201 ctttcaggcg gaaaatggtg attccgcgaa tctggcgatt gcgcccgccg caggctttat
     4261 ggtagtgtcg cggcaccttt gacatacgct cttgcatgcc cccgggcttc gcgcgacttg
     4321 ccccgcgttc gacgatcccg ggaattgctt cgtcccagct tgctgtgacg tcacgtcctc
     4381 gcctgaatcg tcgcatgcgt attaatgttt tcatcccctc gttcaatgcc tccgcgtggc
     4441 gattgcttcc caatcgttgg gatttcgtcg cgttcccgct gattctttgc gtgatcgcca
     4501 tgggggtgg
//
LOCUS       DASTNK010000067        16703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_48040, whole genome shotgun sequence.
ACCESSION   DASTNK010000067 DASTNK010000000
VERSION     DASTNK010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 16703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16703
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48040"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VFR20_05225"
     CDS             <1..303
                     /locus_tag="VFR20_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEU0229952.1"
                     /translation="VEWARKMADYGAGEILLTSMDRDGTKAGFDLELTRAVSDAVAVP
                     VIASGGVGSLQHLADGILEGRASAVLAASIFHYGQHTVQEAKEFMARLGIQVRLTP"
     gene            350..1135
                     /gene="hisIE"
                     /locus_tag="VFR20_05230"
     CDS             350..1135
                     /gene="hisIE"
                     /locus_tag="VFR20_05230"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEU0229953.1"
                     /translation="MSDDTGWLAEIHFDENGLVPAIAQDADTGKVLMVAWMNAESVAE
                     TVATGHAVYFSRSRQRLWRKGEESGHVQTVREIRLDCDGDVLLLQVEQAGGIACHTGR
                     NRCFFRRLDGSTTHGWTITDPVLKAPDAIYQARHGTPPHDHAHETDAPAASGEVLSRL
                     ADTLASRLPRNGGDPATSYAAKLLARGPDAFLKKIGEEATEFVMASKDGVPERIVSEA
                     ADLWFHCLVALTHYGLRPEDVERELLRREGTSGLVEKASRPRE"
     gene            1507..1761
                     /gene="tatA"
                     /locus_tag="VFR20_05235"
     CDS             1507..1761
                     /gene="tatA"
                     /locus_tag="VFR20_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEU0229954.1"
                     /translation="MGGLSIWHWLIVLVIVALIFGTKKLRNFGEDLGGAVKGFKKGMR
                     DAAAEDDAAAPPAAANPQRVQNGETIDVQATEKTDTAAPR"
     gene            1818..2564
                     /gene="tatB"
                     /locus_tag="VFR20_05240"
     CDS             1818..2564
                     /gene="tatB"
                     /locus_tag="VFR20_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEU0229955.1"
                     /translation="MFDVSFTELLLIGIIALIVIGPERLPGVARTIGHLVGRAQRYVN
                     DVKADIQREVDLSEITNLKEQMTDAANSVKATLENATQDLRKPLQDAQDAFKDASASV
                     NNLVTDAKTSAQEAVDSVKAAAAPTQIETAQAPKAVTAESQAAPATETVHSIPVAGSA
                     PATVGEPTTAQAVAAEATTATTAAKTAAAAHADAALTAHESARPAQAGGVANTPPEAP
                     EPTHAPAAESAPAPSPNVETRHPAQPGTPK"
     gene            2561..3370
                     /gene="tatC"
                     /locus_tag="VFR20_05245"
     CDS             2561..3370
                     /gene="tatC"
                     /locus_tag="VFR20_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEU0229956.1"
                     /translation="MSKTAPDDAAVDSTQDTFISHLIELRTRLIRAVASVLIIFVVLF
                     IYPGAGKIYDILAAPMLASLPTGTRMIATGVITPFMVPIKVTLLAAFVLALPIVLYQA
                     WAFVAPGLYKHERRLALPLIISSTFLFLLGMTFCYFVVFRTVFHFIAHFAPHSITPAP
                     DIEAYLNFVMTMFLAFGVAFEVPIFVILLVRTGVVTVEKLRSVRGYVIVGAFIVAAVI
                     TPPDALSQFMLAFPLCLLFEIGVFAASRIKKHKKEEEDSESTDVARDDEAP"
     gene            complement(3501..4655)
                     /locus_tag="VFR20_05250"
     CDS             complement(3501..4655)
                     /locus_tag="VFR20_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU0229957.1"
                     /translation="MRRLWLLFAQTVTICLGVLFIVATLRPDWLRHSPRTVSVAPSPP
                     VATNDLAPVSYAAAVALAAPAVVNIYTSKRVREPIVPLPNDPDLERLFRNLPGFTRSQ
                     KTTALGSGVIVSKDGYILTNYHVIESADAIRVALHDGRQTKARLVGADPDSDLAVLKI
                     DLPDLTVIAFNSKHRVRVGDVVLAIGNPFGVGQTTTMGIVSALGRNRLGLNIYENFIQ
                     TDAAINPGNSGGALVDASGQLVGINTAIYSESGGGSLGIGFAIPASTAHKIMEQIIKT
                     GHVTRGWLGIEPQDITPDLARAFHLSRDDGIIVASVLRRGPAWDAGIRVGDIILKIDG
                     KMVYDTIGFLTEIAPKPPGKDVMLTILRDGRTHEFKVKVGTRPARTRRGE"
     gene            4700..5476
                     /locus_tag="VFR20_05255"
     CDS             4700..5476
                     /locus_tag="VFR20_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU0229958.1"
                     /translation="MNQVSTLELTRWLDQTLQPERFRDYCPNGLQVEGKATIRHIIAG
                     VSASLALLEAAVARGADAVLVHHGWFWKNEDARIRGPKRARLALALRHELNLFAYHLP
                     LDAHPTLGNNAQLARQLGFTIERDADGNPRTCGPDGLVWLGRCDPPKTLAELERAVCN
                     VLQREPLVVGDPQQKIERIAWCTGGAQGMMASAVEAGANMYLTGEASEPTFHLARETG
                     TAFIGAGHHATERYGVRALGQAIAERFGIQVDFIDIDNPI"
     gene            complement(5550..6020)
                     /gene="mscL"
                     /locus_tag="VFR20_05260"
     CDS             complement(5550..6020)
                     /gene="mscL"
                     /locus_tag="VFR20_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU0229959.1"
                     /translation="MATTKGFMAEFREFAVKGNMMDLAIGVIIGAAFAKIIDSLVKDV
                     IMPIVSFILGGKVDFSNMFWVLKAPANYAGPHTYVDMTKAGAVLLGWGNFVTILINFI
                     LLAFVVFCLVKAANNARKRLAKEEAAAPAAPPPTPDDVLVLREIRDLLKSNPQH"
     gene            6306..6950
                     /gene="petA"
                     /locus_tag="VFR20_05265"
     CDS             6306..6950
                     /gene="petA"
                     /locus_tag="VFR20_05265"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEU0229960.1"
                     /translation="MSQDTTQPDGSGAVDPSLPPDPSRRKWVATACTLGGVAGVATVV
                     PFASSFAPSERAKAAGAPVEVDISAIAPGEMHTVAWRGKPVWIVHRTPEQIKNLSKND
                     PYLADPKSLNPDYTPAFARNEYRSRKPEYLVCVGICTHLGCSPNPLFSPGPQPGVPNT
                     WEGGFLCPCHGSYYDLAGRVYKNVPAPDNLLVPPYMFSADGRMLTIGVDEQTKA"
     gene            7044..8432
                     /locus_tag="VFR20_05270"
     CDS             7044..8432
                     /locus_tag="VFR20_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEU0229961.1"
                     /translation="MAGEKSVETTGLLGWVDQRFPLTSTYKKHLSEYYAPKNFNFWYF
                     FGSLALLVLVIQIVTGIFLVMNYKPDAALAFASVEYIMREVPWGWLIRYMHSTGASMF
                     FVVVYLHMLRGLFYGSYRKPRELVWIFGVAIFLCLMGEAFFGYLLPWGQMSYWGAQVI
                     VNLFSAIPFIGPDLSLWIRGDYVVSDATLNRFFAFHVIAIPLVLIGLVVAHLVALHEV
                     GSNNPDGVEIKQGPKDRNGHPVDGIPFHPYYTVHDIMGVAGFLLVFAAIIFFAPEMGG
                     YFLEANNFIPANSLKTPPHISPVWYFTPFYTMLRATTSDFTWVLSAGAIIAAIALFLK
                     GKLSGAWRVITPLILIIIAILMKVLEAKFWGVMTMGTSVLILAFLPWLDRSPAKSIRY
                     RPTWHKFLYAIFIIDFLVLGAIGTQAPNPTLNIISQIGTVIYMGFFFLMPVWSRLGKF
                     KQVPDRITFHPH"
     gene            8475..9308
                     /locus_tag="VFR20_05275"
     CDS             8475..9308
                     /locus_tag="VFR20_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEU0229962.1"
                     /translation="MIMIKKLLGALALSLTCAVAFAEGSAYPLDHAPDRLNDMASLQT
                     GAKLFVNYCLNCHSANMMRYNKLKDLGLTDNEIKKNLLFTGNKVGDLMHVAMQPEDSK
                     KWFGTSPPDLSVIARAKGIDYIYTYLRSFYRDTAQPSGWNNLVFPKVAMPNVLWQLQG
                     PRAMHRVTIEKTENAAGVEHWQRTTSDYDSQGFSTIKTEALKGYDRGPVDNVTYSGIN
                     STQRAAFDNDAADISNFLQWMSEPGQQFRKELGVWVMLFLMLFFLVAWRLNAAYWKNV
                     K"
     gene            9482..10090
                     /locus_tag="VFR20_05280"
     CDS             9482..10090
                     /locus_tag="VFR20_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0229963.1"
                     /translation="MMVLYSGTTCPFSQRCRFVLFEKGMDFEIRDIDLYNKPEDIAVM
                     NPYGQVPILVERDLILYESSIINEYIDERFPHPQLMPADPVMRARTRLFLYNFEKELF
                     VHVSTLENRATPDPKALDQARQNIRDRLSQLAPMLLKNKYMLGDEFSMLDVAVAPLLW
                     RLDHYGIELPKNAAPLQKYAERIFSRPAYIEALTPSEKVMRR"
     gene            10096..10566
                     /locus_tag="VFR20_05285"
     CDS             10096..10566
                     /locus_tag="VFR20_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEU0229964.1"
                     /translation="MSESSTKPYLIRALHEWCTDNGYTPHIVVTVDANTVVPRAHIHD
                     GQITLNIGALATNRLILGNDYIEFEARFSGMTEHIYVPVATVSAIYARETGAGMGFEV
                     AESQPYGTDSANAGGGTEQTPATVKSVPTATEPGTAANPGHDDDPKRPRLTIVK"
     gene            complement(10597..11358)
                     /locus_tag="VFR20_05290"
     CDS             complement(10597..11358)
                     /locus_tag="VFR20_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU0229965.1"
                     /translation="MDIPANQFKRNLLAKRHQVGMWVSLASRYSAEIVAGSGFDWLVI
                     DTEHAPNDIESVLSQLQSMAAHPVSPIVRPTCNDAVQLKRVLDVGAQTVLVPYVQDAD
                     EAALAVAGTRYPPRGHRGAGGVMRASGFGRVRDYSQRCEAELCVVVQIESRKGLDNLE
                     AIAAVDGVDGLFIGPADLAASLGHTGDAGHPEVQSAIADAMRRILACGKAPGILALEE
                     KQARRYMELGSLFTAVALDVAVLARETARLAALYK"
     gene            complement(11441..12244)
                     /locus_tag="VFR20_05295"
     CDS             complement(11441..12244)
                     /locus_tag="VFR20_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0229966.1"
                     /translation="MKQARFTLALASAAVALACAVPAAHANTLDQVMKSKVLRVAIDL
                     NAPPYGMKNSSLKEVGSDVDTARLIAKDLGVKLEIVPTTQANRIPFLLTNKADVVISS
                     LSVTDARKKVIDFTIPYAAIQSVIAAPKDMAIGSMKDLAGKTVVTARGTTNDNNVTRL
                     APPGTNIVRFENDATAITAIISGQAKIFSTAPAIIAALNKKDPAKALQIKVVMSTSML
                     GFGVTKNNTALKDKLNELIRKDLKNGELNAIYKKYHGADLPKDVMSEGA"
     gene            complement(12307..13041)
                     /locus_tag="VFR20_05300"
     CDS             complement(12307..13041)
                     /locus_tag="VFR20_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU0229967.1"
                     /translation="MSEVVRLSNVHKRFGDNHVLKGIDLSVQKGEIVAIIGQSGSGKS
                     TALRCMDHLEHIDEGEINVCGHTFGAGIPRRDLKALRQDVGIVFQSYNLFPHLTVEQN
                     ITLAPVSVRKMSKPQAKKVAHQVLSQVGLAEKASFYPEQLSGGQQQRVAIARSLAMGP
                     KLMLFDEVTSALDPQLTGEVLRVIEKLAREGMTMVLVTHEMAFAMKLAHRVVFMHEGR
                     IHEEGSADILRNPATPELRQFVSNGL"
     gene            complement(13067..13714)
                     /locus_tag="VFR20_05305"
     CDS             complement(13067..13714)
                     /locus_tag="VFR20_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU0229968.1"
                     /translation="MNSAQIAYLIQGAWGTVLLSVLTFVFGGMIGLAVALMRVSPRTP
                     VRGAAFTYIQVIQGTPLLVLMGVCFYGPSIMGYESVPALVAAVLALSVQSGAFLGEIW
                     RGCIESVPRNQWEAAECLGLNKVQRMFKVVLPQAVRIATPPTVGFMVQIVKNTSFASL
                     VIGFAELSYNAKILNNATFKPFLYFGVAAVLYFIICYPLSWLSLKLERKLTLAQS"
     gene            complement(13725..14408)
                     /locus_tag="VFR20_05310"
     CDS             complement(13725..14408)
                     /locus_tag="VFR20_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU0229969.1"
                     /translation="MTYQFDFASVLAYWHLFVSGVETTLVMSFWSTVAGFVMGVVCAI
                     ARSSSYPVLRKVVGAYVEVIRNTPLIIQSYFFIFGLSSVGLTMPILVGAITALVINIT
                     AYTCEIVRAGIESVPRSQREAAECLGLSPLRVYWHVILVPALERVYPALTSQFILLML
                     VTSILSAVGTDDLFGVAGRVQSLTFRNFEVFIVLWVVYLALSFLVRALFWGLGKAMFV
                     RKRRLGTLL"
     gene            14684..15442
                     /locus_tag="VFR20_05315"
     CDS             14684..15442
                     /locus_tag="VFR20_05315"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEU0229970.1"
                     /translation="MTSAPHHTDHTGKRNYLHIADMLRQAIGAMDLDPSGRLPSEREL
                     AQLLGVSRPSLREALIVLELRQEIEIRMGSGIYLTHPTEPGDSAGAPPPQDDALDPLL
                     QKLGDSPRDVNQMRFFLESGVAAHAARFISTAQLKQLKKSLTDMRSALAKKSRGSIKI
                     IADADRLFHTTLASCTDNDLLVQTLEYLFDQRYTPVAEAMHKHYEDPCSWEFAVEEHQ
                     DIYDAIAAHDPLQAQAAMQRHLSRAHARLMPLIG"
     gene            15475..>16703
                     /locus_tag="VFR20_05320"
     CDS             15475..>16703
                     /locus_tag="VFR20_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014899139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEU0229971.1"
                     /translation="MPQTTIRLNEHDNVIIATQQLMQGNTAEGITVTGLIPAGHKMAA
                     EAIAQGSPVRRYNQIIGVATKDIKPGQHVHTHNMEMLDFDRDYNFCAHKKAVPINDQA
                     DHFLGIVRPDGRVATRNYIGILTSVNCSATVAKSIADHFRRDINPAALADFPNVDGVV
                     ALPHSTGCGMGVKGLFMDTLRRTLIGYMRHANFHSVLMVGLGCETNQIPALMATGGLE
                     ESSRLQTFTIQDTGGTAKSVAKGIAKVKEMLPAANQVTRTPVSASHIVVGLQCGGSDG
                     YSGITANPALGAAVDRLVRAGGAAILSETPEIYGAEHLLTQRAVSREVGEKLVERIKW
                     WEDYCSRNFAELNNNPSPGNKAGGLTTILEKSLGAVAKAGTTNLNEVYQYAEPVTAKG
                     MVFMDSPGYDPVSATGQV"
BASE COUNT         3250 a         5564 c         4930 g         2959 t
ORIGIN      
        1 gttgaatggg cccggaaaat ggccgactac ggcgccggtg agatcctgct caccagcatg
       61 gatcgcgacg gcaccaaggc cggcttcgac ctggaactga cgcgcgccgt gtccgacgcc
      121 gtggccgtgc cggtcatcgc ctcgggtggc gtcggcagcc tgcagcacct ggccgacggc
      181 attctagaag ggcgcgccag cgcggtgctg gccgccagca tcttccacta tggccagcac
      241 accgtccagg aggccaagga attcatggcc cgactgggca tccaggtacg attgacacca
      301 tgagcggcca ggcatcctgg cgcaatgggc aacaaggcac gactgacaca tgagcgacga
      361 cacgggctgg ctggccgaga tccacttcga cgagaacggg ctggtgcccg ccatcgccca
      421 ggacgccgac accggcaagg tgctcatggt ggcctggatg aacgccgaat ccgtggccga
      481 aaccgtggca acgggccacg ccgtgtactt ctcgcgctcg cgccagcgcc tgtggcgcaa
      541 gggcgaagaa tccggccatg tgcaaacggt gcgcgaaatc cgcctcgact gcgatggcga
      601 cgtgctgctg ctgcaggtgg aacaggctgg cggcatcgcc tgccacaccg gccgcaaccg
      661 ctgtttcttc cgtcgcctcg acggcagcac cacgcatggc tggaccatca ccgatccggt
      721 tctgaaggca cccgacgcca tttaccaggc gcggcacggt acaccccccc acgaccacgc
      781 acacgaaacg gacgcgccgg ccgcatcggg cgaggtcctg tcgcgcctgg ccgacacgct
      841 ggcatcacgc ctgccccgca acggtggcga tcccgccacg tcctacgcgg ccaagctgct
      901 ggcccgcgga cccgatgcct tcctgaagaa aatcggcgag gaagccaccg aattcgtcat
      961 ggcatccaaa gacggcgtgc cagaacgcat cgtcagcgaa gccgccgact tgtggttcca
     1021 ctgcctggtc gcgctgacgc actacgggct gcgccccgaa gacgtggaac gtgaactctt
     1081 gcgtcgcgaa ggcacgtcgg gtctcgtcga aaaggccagc cggccacgcg aataggacgg
     1141 gcggcaaggc agggtttacg cgaggaatcg ccttgttccg gaactgtgat gcgtgaaagc
     1201 tgtcccttca taaagggacg gcgcggcggc gacgcgcacg gctcgccagc gcaggggcat
     1261 agggaaaacc ttactcgcgc aggtggggtc ggcggcgggg atcccggttt ttcgtgatat
     1321 aaatccggct gcccctggtc atttgccgtc aaacccaaag cggcctatcg tgtaagcaag
     1381 tatgcggggc atcggccaga acgatgctcg ctggttgggt agaatggtgg tattgacttt
     1441 cacattatcg ggggcccgag cccccacaag cgtaacgcgg cgtccgccgc tcgacggaga
     1501 cacatcatgg gtggcttaag catttggcac tggctgatcg ttctggtgat cgtagccctt
     1561 attttcggca caaagaaact gcgcaacttc ggtgaagacc tgggcggcgc ggtgaagggc
     1621 ttcaagaaag gcatgcgcga cgccgccgcc gaagacgacg ccgcggcccc tcccgccgcc
     1681 gccaatccgc aacgggtgca gaatggcgaa accatcgacg tgcaggctac ggaaaagacc
     1741 gacaccgccg ccccccgcta agcgcccgct cgcgttgcgt ccggcttcat tcctctgttc
     1801 cgcaacctga ttgatccatg ttcgacgtca gcttcaccga actactcctc atcggcatca
     1861 ttgccctgat cgtcatcggc cccgagcgtc tgcccggcgt cgcgcgcacg atcgggcact
     1921 tggtggggcg tgcccagcgc tacgtcaacg acgtcaaagc cgacatccag cgtgaagtcg
     1981 acctgagcga gatcaccaac ctgaaagagc agatgaccga cgcggccaac agcgtcaagg
     2041 ccacgctgga aaacgccacg caggatttgc gcaaaccgct gcaagacgcg caggacgcct
     2101 tcaaagacgc ctccgcctcc gtcaacaacc tcgtgaccga cgccaagacc tcggcacagg
     2161 aagcggtcga cagtgtcaag gccgctgcgg cgcccacgca aatcgagacc gcgcaggcgc
     2221 ccaaggccgt gacggctgaa tcccaggctg ccccggccac cgaaacagtc cattccatac
     2281 ccgtggccgg aagcgcgcca gccaccgtcg gcgagccaac caccgcccaa gcagtcgccg
     2341 ctgaagcaac cacagccacc acggcagcca aaacggccgc tgcggcacat gccgacgcag
     2401 cactcacagc ccacgaatcg gcccggccgg cgcaggccgg cggggtcgcc aacacgccgc
     2461 ccgaggcgcc tgagccaaca cacgcgcccg ctgcggaatc ggcgcccgcc ccctccccca
     2521 acgtggaaac ccgacatccc gcccaacccg ggacgcccaa gtgagcaaga ccgcccccga
     2581 cgatgccgcc gtggactcca cgcaggacac cttcatttcg cacctgatcg aactacgcac
     2641 ccgcttgata cgggccgtgg cgtcggtgct gatcattttc gtggtgctgt tcatctaccc
     2701 aggcgcgggc aagatctacg acattctcgc cgcgcccatg cttgcgtcgc tgcccacggg
     2761 cacgcgcatg atcgccaccg gcgtcatcac gccgttcatg gtgcccatca aggtcaccct
     2821 gctggcggcc ttcgtgctgg cgctgcccat cgtgctgtac caggcctggg cgttcgtggc
     2881 gccgggtctc tacaagcacg aacggcgcct ggccctgccg ctgatcatct ccagcacctt
     2941 cctgttcctg ctgggtatga cgttctgtta tttcgtcgtg ttccgcacgg ttttccattt
     3001 catcgcgcat ttcgcgccgc actccatcac acccgcgcct gacatcgagg cctacctgaa
     3061 cttcgtgatg acgatgttcc tggctttcgg cgtggctttc gaagtgccca tcttcgtcat
     3121 cctgctcgtc agaaccggtg tggtcacggt cgagaaactt cggtcagtcc gcggttacgt
     3181 gatcgttggc gccttcatcg tggccgccgt catcactccc cccgacgctc tcagccagtt
     3241 catgctggcc tttccgttgt gcctgctgtt cgagatcggg gtcttcgcgg ccagccgcat
     3301 caagaagcac aagaaagaag aagaggacag cgagtccacc gacgtggcgc gcgacgacga
     3361 ggcgccttga gcaggtagcc accgcgggcg gcacgaaaac cccgcccgcg ttcccaaaga
     3421 gcgacccggc gctacttctc atgccgtatc aacgcgcgcc gcctcaacgt gccggcacgc
     3481 tgcctgaccc tcaccaacga ttactcgccg cgccgcgtcc gggccggccg ggtgccgacc
     3541 ttgaccttga attcgtgggt gcgaccgtca cgcaatatgg tcagcatgac gtctttgccc
     3601 ggcggcttgg gcgcgatctc ggtcaggaac ccgatggtgt cgtagaccat cttgccgtcg
     3661 atcttcagga tgatgtcacc gacgcgaatg cccgcgtccc aggccggccc acgcctgagc
     3721 acgctcgcca cgatgatgcc atcgtcgcgg ctaaggtgga acgcgcgcgc caggtcgggc
     3781 gtgatgtcct gtggctcgat gcccagccag ccgcgtgtca cgtggccggt cttgatgatc
     3841 tgctccatga tcttgtgcgc cgtcgaggcg ggaatcgcga agccgatgcc cagcgagccg
     3901 ccgcccgact cggagtagat ggccgtgttg atgccgacga gctgccccga ggcgtcgacc
     3961 agcgccccac ccgagttgcc ggggttgatg gccgcgtcgg tctggatgaa gttttcgtag
     4021 atgttgaggc ccaaccgatt gcgccccagc gcggacacga tgcccatggt ggtggtctgc
     4081 cccacgccga acgggttgcc gatggccagc accacatcac cgacgcgcac ccggtgcttg
     4141 ctgttgaacg cgatgacggt cagatccggc agatcgatct tcagcaccgc cagatcgctg
     4201 tcggggtccg cccccaccag cctggccttg gtctgacgcc catcgtgcaa tgccacacga
     4261 atggcgtcgg cggattcgat gacgtgatag ttggtgagga tgtagccatc tttgctgacg
     4321 atcacccccg accccagtgc cgtggtcttc tggctgcgcg tgaagcccgg aaggttgcgg
     4381 aacagacgtt cgaggtcggg gtcgttgggc agcggcacga tgggctcgcg cacgcgtttg
     4441 ctggtataaa tattgaccac ggcaggcgcg gccagcgcga cggcggcggc atatgaaacc
     4501 ggggctagat cattcgtggc gacgggcggc gacggcgcca cggacaccgt gcggggcgaa
     4561 tgcctcagcc agtctggccg caatgtcgcc acgatgaaca acaccccaag gcagatcgtc
     4621 actgtctggg cgaacaacaa ccacaatcga cgcatacgtt tcgctttccc acaagacccg
     4681 cacaaccaca aggccagaca tgaaccaggt ctccaccctc gaactgacgc gctggttaga
     4741 ccagacactg caacccgaac gcttccggga ttactgcccc aatgggttgc aagttgaagg
     4801 caaagccacg attcgtcata ttatcgccgg agtcagcgct tcgctggcac tgctcgaggc
     4861 cgccgtcgcg cgcggcgccg acgcggtcct ggtgcatcat gggtggttct ggaaaaatga
     4921 agacgcgcgc attcgcggcc ccaagcgcgc gcgcctggcg ctggcgcttc ggcacgagct
     4981 gaatctgttc gcctaccacc tgcccctgga tgcgcacccc acgctgggca acaatgcgca
     5041 actggcccgg caactggggt tcaccatcga gcgcgacgcc gacggcaacc cccgcacctg
     5101 cggccccgac ggcctggtct ggctggggcg ttgcgatccc ccgaaaaccc tggccgaact
     5161 cgaacgcgcc gtttgcaatg tcttacaacg cgagcctttg gttgttggcg atccacaaca
     5221 aaaaatcgag cgcatcgcct ggtgcaccgg cggcgcgcag ggcatgatgg cgtccgccgt
     5281 ggaggccggc gcgaacatgt acctgacggg cgaggcgtcg gagcccacgt tccatctggc
     5341 gcgggaaacc ggcaccgcgt tcatcggcgc cggccaccac gccaccgagc gctacggcgt
     5401 gcgggcgctg ggccaggcca tcgcagaacg attcggcata caggtggatt tcatcgacat
     5461 cgacaacccc atctgacacg gtcagagagg cgtggccacg ttgggccgcg cgccgatatg
     5521 gccgcggccc aacgacgggc cgggatcgct tagtgttgcg gattgctctt gagcaggtcg
     5581 cggatttcac gcaggaccag cacgtcgtcc ggagtgggcg ggggcgcggc cggcgcggcg
     5641 gcctcttcct tggcgaggcg cttgcgcgcg ttgttggcgg ccttgaccag gcagaacaca
     5701 acgaacgcca gcaggatgaa attgatgaga atggtgacga agttccccca ccccagcaac
     5761 acggcgccgg ccttggtcat gtccacatag gtgtgcggcc ccgcgtaatt ggcgggcgcc
     5821 ttcaataccc agaacatatt ggaaaaatcg accttgccgc ccaggatgaa gctgactatc
     5881 ggcatgatga cgtctttcac caacgagtcg atgatcttgg cgaacgcggc gccgatgatg
     5941 acgccgatgg ccagatccat catgttgccc ttgaccgcga actcgcggaa ttccgccatg
     6001 aacccctttg ttgtagccat gtctttctcc tgtgacaatt caccgttggt cctgctgacg
     6061 ctataatacg cggttgatat ggctttgcgc ttgttaaata gcgtaaagac cgtgcctcgt
     6121 tgttgaacga aatctgtttg ctgcacgaaa tgtgtgggtc tgctacgcgg accatccggg
     6181 gggagcaaag cgggagggca caccttggga aacacacacc gggcgcatcg cggatggcgg
     6241 gcataccggc gcgggccatt catcgcaagg caaggttgca aacaaggaca caacaaggac
     6301 acagaatgag tcaggatacg acacagcccg atggttccgg cgcggttgac ccaagcctcc
     6361 cgccagatcc ttcgcgccgt aagtgggttg ccaccgcctg tacgctgggt ggggtggctg
     6421 gcgttgcaac agtagtccct ttcgccagct catttgcccc atcggaacgc gccaaggccg
     6481 cgggtgcccc ggttgaagtc gatatctcgg ccatcgcccc cggcgaaatg cacacggtgg
     6541 cgtggcgcgg caaaccggtc tggatcgtgc accgaacacc cgagcagatc aagaacctgt
     6601 ccaagaacga cccctacctg gccgatccca aatccctcaa ccccgactac acaccggctt
     6661 tcgcccgcaa cgaataccgg tcgcgcaaac ccgaatatct cgtttgcgtc ggcatctgca
     6721 cgcacctggg ctgctcgccc aatccgctct tctcacccgg cccgcagccc ggtgtcccga
     6781 atacctggga gggcggcttt ctctgcccct gccacgggtc ctattacgat ctggcgggcc
     6841 gtgtctacaa gaacgttccc gcgccggaca accttctggt tcctccgtac atgttctccg
     6901 ccgacggccg catgctcacc atcggcgtcg acgaacaaac caaggcataa ggcaaggcgc
     6961 ccgtcaccgg gcctcgatca ccgcgcggcg cgacgccaag tcaccgtccg cctcagtaag
     7021 atagaagaaa aggagccgtt ctcatggctg gcgagaaatc cgtcgaaacg acagggcttt
     7081 tgggttgggt ggaccagcgc tttccgctga cgtccacgta caagaagcac ctgtccgaat
     7141 actacgcacc caagaacttc aatttctggt atttcttcgg atcgctcgcc ctgctggtgc
     7201 tggtcatcca gatcgtcacg ggcattttcc tggtcatgaa ctacaagccc gacgccgcgc
     7261 tcgcgttcgc gtcggtcgag tacatcatgc gcgaagtgcc ctggggctgg ctcattcgct
     7321 acatgcactc cacgggcgcc tcgatgttct tcgtggtggt ctacctgcac atgctgcgcg
     7381 gattgttcta cggttcgtat cgcaagccgc gcgaactggt ctggatcttc ggcgtggcca
     7441 tcttcctgtg cctcatgggc gaagccttct tcggctatct gctgccgtgg ggccagatgt
     7501 cgtactgggg cgcgcaggtc atcgtcaacc tgttctcggc cattcccttc atcggacccg
     7561 acctctccct gtggatccgt ggcgactacg tcgtgtccga cgccaccctg aaccgtttct
     7621 tcgccttcca tgtcatcgcc atcccgttgg tcctcatcgg cctggtggtc gcccacctgg
     7681 tggcgctgca tgaagtgggt tccaacaacc ccgacggcgt cgaaatcaag cagggcccca
     7741 aagaccgcaa cggccacccc gtcgacggca tccccttcca cccttactac acggtgcacg
     7801 acatcatggg cgtggccggc ttcctgctgg tgttcgcggc catcatcttc ttcgcgcctg
     7861 aaatgggcgg ctatttcctt gaagccaaca acttcatccc ggccaactcg ctcaagacgc
     7921 cgccgcacat ctcgccggtc tggtacttca caccgttcta caccatgctg cgcgccacca
     7981 ccagcgactt cacctgggtg ctgtcggccg gcgcaatcat cgcggccatc gccctgttcc
     8041 tcaagggcaa gctctcgggc gcctggcgcg tgatcacgcc gctcatcctc atcatcatcg
     8101 ccattctcat gaaggtcctc gaagccaagt tctggggcgt catgaccatg ggcacgtcgg
     8161 tgctcatcct ggccttcctg ccgtggctcg accgctcgcc ggccaagtcc atccgctacc
     8221 ggcccacatg gcacaagttc ctctacgcca tattcatcat cgatttcctg gtactgggcg
     8281 cgatcggcac gcaggccccg aaccccaccc tgaacatcat cagccagatc ggaaccgtga
     8341 tctacatggg cttcttcttc ctgatgcccg tctggagtcg tctcggcaag ttcaagcagg
     8401 tgcctgaccg aatcacgttc cacccccact aaggttcagc cgtcgcacat cgaggcccgt
     8461 caaatatcta tggaatgatc atgatcaaaa agctgcttgg tgccctggcg ttgtcactga
     8521 cttgcgcggt tgcgttcgcg gaaggcagtg cgtatccgct ggaccacgcc cccgaccgac
     8581 tcaacgacat ggcttcgctg caaaccggcg ccaagttgtt cgtgaactac tgcctgaatt
     8641 gccatagcgc caacatgatg cgctacaaca agctgaaaga ccttggcctg accgacaacg
     8701 aaatcaagaa gaacctgctg ttcaccggca acaaggtggg cgacctgatg cacgtcgcca
     8761 tgcagccgga agactccaag aagtggttcg gcacctcgcc ccccgacctg tccgtcatcg
     8821 cccgcgccaa gggcatcgac tacatttaca cctatctgcg ctcgttctat cgcgatacgg
     8881 cccaaccctc gggctggaac aatctggtgt tccccaaggt cgccatgccc aacgtgctgt
     8941 ggcagttgca ggggccgcgc gcgatgcacc gcgtgaccat cgaaaagacc gagaacgccg
     9001 ccggcgtcga gcattggcag cgcaccacgt ccgactacga cagccagggc ttttccacga
     9061 tcaagaccga ggcgctcaaa ggctatgatc gcggccccgt cgacaacgtc acgtactcgg
     9121 gcatcaacag tacccagcgc gcggcgttcg acaacgacgc cgccgacatc agcaatttcc
     9181 tgcaatggat gtccgaaccc ggccagcaat tccgcaagga actgggtgtc tgggtcatgc
     9241 tgttcctgat gctcttcttc ctggtggcgt ggcggctcaa cgccgcttac tggaaaaacg
     9301 tcaaataacc cggtgtcgtg attcacggca ccagccgggc gtctcgcggc gcccggcctt
     9361 cgcggcaggc cgattaagct gccattcaag aaatgggcgt agtgtggtta cgcccgtttt
     9421 cccttttcag gggctgcgtt ccgcgccccg gttttcaccc caatagactg gaatccgcaa
     9481 catgatggtg ctctattccg ggacgacatg cccgttttcc caacgctgcc gattcgtgct
     9541 ttttgaaaaa ggcatggatt ttgaaatccg cgacatcgac ctttacaaca agcctgaaga
     9601 catcgccgtg atgaatccgt acggccaggt gcccatcctg gtcgagcgtg acctgattct
     9661 gtacgaatcc agcatcatca acgagtacat cgacgaacgc tttccgcatc cgcaactcat
     9721 gccggccgat ccggtcatgc gtgcgcgcac gcgcctgttc ctgtacaact tcgaaaagga
     9781 actgttcgta cacgtctcca cgctcgaaaa ccgcgccacg ccagacccca aggccctgga
     9841 ccaggcacgc cagaacatcc gcgaccgcct gtcgcaactg gcgcccatgc tgctcaagaa
     9901 caagtacatg ctgggcgacg agttctccat gctggacgtg gccgtcgccc ctctgttgtg
     9961 gcgcctggac cactacggca tcgaactgcc caagaacgcg gcgccattgc agaaatacgc
    10021 cgagcgcatc ttctcgcgcc ccgcctatat cgaagcgctg acgccgtccg aaaaagtcat
    10081 gcgtcgttga caccgatgtc agaatcctcc accaagccct acctgatccg cgcgctgcat
    10141 gaatggtgca ccgacaacgg ctataccccg catatcgtgg ttaccgtcga tgccaacacc
    10201 gtcgtgccgc gcgcccacat ccacgatggc cagatcacgc tgaacatcgg cgccctggcc
    10261 acgaatcgtc tcatcctggg caacgactac atcgaattcg aggcccgctt cagcggcatg
    10321 accgagcaca tttatgtgcc ggtcgccacg gtctcggcca tctacgcccg ggaaacaggg
    10381 gccggaatgg ggttcgaagt ggcggaatcg caaccctatg gcaccgattc agcgaatgcc
    10441 gggggcggca ccgagcaaac gccggcaacc gtcaaaagcg tccccaccgc caccgaaccc
    10501 ggcacagccg caaaccccgg ccacgacgac gacccgaagc gcccgcgcct gacaatcgtg
    10561 aagtagcggg ccacgacgtg tcggtggccc gcgcccttat ttgtacagcg cggccagccg
    10621 ggcggtttcg cgcgccagca ccgccacatc gagcgcgacg gccgtgaaca gcgaccccag
    10681 ttccatgtag cggcgcgcct gtttttcttc cagcgccagg atgcccggcg ccttgccgca
    10741 ggccaggatg cgccgcatgg catcggcaat ggccgattgc acctctgggt ggccggcatc
    10801 gcccgtgtgg ccaagactcg cggccaggtc cgccggcccg atgaacagcc cgtccacccc
    10861 atccaccgcg gcaatggctt ccaggttgtc caaccccttg cgcgattcga tttgcaccac
    10921 gacgcagagc tcggcctcgc agcgctgtga gtagtctcgc acacggccga agccgctggc
    10981 gcgcatcacc ccgcccgcgc cacggtgccc gcgcggcggg tagcgcgtgc cggccacggc
    11041 cagcgcggct tcgtcggcat cctgcacata aggcaccagc acggtctggg cgccgacatc
    11101 gagcacacgc ttgagctgca ccgcatcgtt gcaagtgggc cgcacgatgg gcgacaccgg
    11161 atgggccgcc atggattgca gttgcgacag cacgctttct atgtcgttgg gcgcgtgctc
    11221 ggtgtcgatg accagccagt cgaagcccga tccggcgacg atctccgcgc tatagcggct
    11281 ggccaacgat acccacatgc caacctgatg ccgctttgcg agaagattgc gtttgaactg
    11341 gttggctgga atgtccatca tcgtcctttg catgaatgcc ggccacgccc ggggttcaca
    11401 tcacccgccc ggcgtggcct gcgatacggc gcgctatgcg tcaagcgcct tcggacatga
    11461 catccttggg cagatcggcc ccatgatact tcttgtagat ggcgttgagc tcgccgttct
    11521 tcaggtcctt gcggatcagt tcattcaact tgtctttcag ggccgtattg tttttggtca
    11581 caccaaagcc cagcatggac gtggacatga ccaccttgat ctgcagcgcc ttggcggggt
    11641 ctttcttgtt cagggcggcg atgatggccg gcgcggtgga gaatatcttc gcctggccgc
    11701 tgatgatggc ggtgatggcg gtggcgtcgt tttcgaaacg gacgatgttc gttccgggcg
    11761 gcgcgaggcg tgtcacgttg ttgtcattgg tggtgccgcg ggccgtgacc acggttttgc
    11821 cggccaggtc tttcatcgaa ccgatggcca tgtccttggg cgccgcgatc accgattgga
    11881 tggcggcata ggggatggtg aagtcgatga ccttcttgcg cgcatcggtg accgacaagc
    11941 tcgagatcac cacatcggcc ttgttggtca gcaggaacgg gatgcggttg gcctgggtgg
    12001 tgggcacgat ttcaagcttg acgcccaggt ccttggcgat cagacgggcg gtgtccacgt
    12061 ccgaccccac ctctttcagc gagctgttct tcatgccata tggcggagcg ttcaagtcaa
    12121 tcgccacgcg caacactttg ctcttcatca cttgatcgag cgtgttggca tgcgcggcgg
    12181 gcacagcgca cgccagtgcc acggcggcgc tggccagcgc cagagtgaat cgtgcttgct
    12241 tcatcaaaag tctccttggt gtggtggggc gccggcggga aaacccccgc tggcgtggaa
    12301 acggcgtcaa agcccgttgc tgacgaactg cctgagttct ggtgtggcgg ggttgcgcag
    12361 gatgtcggcc gaaccctctt catggatccg gccctcgtgc atgaacacca cgcgatgcgc
    12421 caatttcatg gcgaacgcca tttcatgggt gaccagcacc atggtcatgc cttcgcgcgc
    12481 gagcttttca atgacgcgca ggacttcgcc cgtgagttgc ggatccagcg ccgaggtgac
    12541 ctcatcgaac agcatcagct ttggacccat ggccagcgaa cgcgcgatcg ccacccgctg
    12601 ctgctgcccc ccggagagct gttcgggata gaacgacgcc ttttccgcca ggccgacctg
    12661 tgacagcacc tggtgcgcca ctttcttcgc ctgaggcttg gacatcttgc gcaccgatac
    12721 cggggccagc gtgatgttct gttcgaccgt gaggtgcggg aacaggttgt agctctggaa
    12781 tacgatgccc acgtcctggc gcagcgcctt caggtccctg cgggggatgc cagcgccgaa
    12841 cgtgtggccg cacacgttga tctcgccctc gtctatgtgt tcgagatggt ccatgcagcg
    12901 caacgccgtg ctcttgccgg agccgctctg gccgatgatg gcgacgatct cgcccttttg
    12961 cacagacagg tcgatgcctt tcaggacatg gttgtctccg aagcgcttgt gtacgttgct
    13021 gagacggacg acttccgaca tggcggtttc cttggtgggt gacggttcag ctttgggcga
    13081 gcgtcagttt gcgctccagc ttcaggctga gccaggacag ggggtagcag atgatgaaat
    13141 acaggacggc cgccacgcca aaatacagga atggtttgaa cgtggcgttg ttcaggatct
    13201 tggcgttgta cgacagttcg gcgaagccga tgacgagcga tgcgaacgac gtgttcttga
    13261 cgatctgcac catgaacccc acagtgggcg gggtggcgat gcgcacggcc tgcggcaaga
    13321 caaccttgaa catgcgctgc accttgttca ggcccaggca ctcggccgcc tcccattggt
    13381 tgcggggaac cgattcgatg cagccgcgcc agatttcgcc caggaaggca ccgctttgca
    13441 ccgacagcgc cagcacggcg gccaccaggg cgggcaccga ttcatacccc atgatcgacg
    13501 gcccgtagaa acacacgccc atgagcacca gcagcggggt gccctggatg acctggatgt
    13561 aggtgaacgc ggcgccgcgc acgggcgtgc gcggcgacac ccgcatgagg gcgaccgcta
    13621 gcccgatcat ccccccgaac acgaacgtga ggacggacag cagcaccgtg ccccatgccc
    13681 cctggatgag ataggcgatt tgcgcggaat tcatgaaccc gccctcacag cagcgtgccg
    13741 agacggcgtt tgcggacgaa catggccttg cccagccccc agaacagggc gcgcacgagg
    13801 aacgacaacg ccagatagac gacccacagg acgatgaaca cctcgaagtt gcggaaggtc
    13861 agcgactgga cccggccggc aacgccgaac agatcgtcgg tgccgaccgc cgacaggatg
    13921 ctggtgacca gcatgagcag gatgaactgg ctggtgaggg cgggatagac gcgctctagc
    13981 gccggcacca ggatgacgtg ccagtagacg cgcaggggcg acagacccag acattcggcg
    14041 gcctcgcgct ggctgcgcgg caccgactcg atgcccgcgc gcacgatctc gcaggtgtag
    14101 gcggtgatgt tgatcaccag cgcggtgatg gcccccacca gaatgggcat ggtcagaccc
    14161 acgctggaca ggccgaagat gaagaaataa ctctggatga tgagcggcgt gttgcggatg
    14221 acctctacat aggcgcccac gactttgcgc aacacgggat aggacgagct gcgggcgatg
    14281 gcgcacacga cccccatgac gaagccggcg actgtcgacc agaacgacat gaccagcgtg
    14341 gtctccaccc cggagacgaa caaatgccag taggcaagca ctgaggcgaa atcgaattga
    14401 tacgtcattg ccgggcttaa ggtgctgtgt ggtgggttcg aatactcgac tccggcgcct
    14461 gtgcccgcca cgcggcagca cacttgccca caccgaaaaa atgggaatcg ggcatatcct
    14521 accttttaaa attggtaaaa ccacttatcg aaaaccctag atttcaaata atttcaatta
    14581 ttggtttgac cacttatcgc gatttaccta tcattgccta tcatgggttt gatccctgtc
    14641 tggctcggtc gctccagcca ccattcgaac accgttcgct cacatgactt ccgctccgca
    14701 tcacactgac cacaccggca agcgcaacta cctgcacatc gccgacatgc tcaggcaggc
    14761 gataggcgcc atggacctcg accccagtgg tcgattgccg tccgaacgcg aactcgccca
    14821 gttgctgggc gtgtcgaggc ccagcctgcg cgaggcgctc atcgtgctcg agctgcggca
    14881 ggagatcgag atccgcatgg ggtcgggtat ctacctgacc caccccaccg agccaggcga
    14941 cagcgccggc gcgccaccgc cccaggacga cgcactcgat ccgctgctgc aaaagctggg
    15001 cgacagcccc cgtgacgtca accagatgcg gttcttcctg gaaagcggcg tggccgccca
    15061 cgcggcacgc ttcatcagca ccgcgcaact gaagcaactg aagaaaagcc tgaccgacat
    15121 gcggtcggcg ctggcaaaga aaagccgcgg ctccatcaag atcatcgccg acgccgaccg
    15181 gctgttccac accacactgg cctcatgcac cgacaacgac ctgttggtcc agacgctgga
    15241 atatctgttc gaccagcgct acacacccgt ggccgaagcc atgcacaagc attatgaaga
    15301 cccgtgctcc tgggaattcg cggtcgaaga acaccaggac atctacgacg ccatcgcggc
    15361 ccacgacccc ctgcaggcac aggccgccat gcaacgccat ctttcacggg cgcacgctcg
    15421 cctcatgccc ctcatcggtt gacgcccgcc cccatccact tatccgagcc atccatgcca
    15481 cagaccacca ttcgcctcaa cgaacacgac aacgtcatca tcgccaccca gcagctcatg
    15541 cagggcaaca ccgccgaagg catcacggtg acggggctga ttccggccgg ccacaagatg
    15601 gccgctgaag ccattgcgca aggcagtccg gtgcgacgct acaaccagat catcggcgtg
    15661 gccaccaaag acatcaaacc cggtcagcac gtgcacacgc acaacatgga aatgctggat
    15721 ttcgaccgcg actacaactt ctgcgcgcac aagaaagcgg tgcccatcaa tgaccaggcc
    15781 gaccacttcc tgggcatcgt gcgcccggac ggccgggtcg ccacgcgcaa ctacatcggc
    15841 atcctgacct cggtgaactg ttcagccacc gtggccaaat ccatcgccga ccatttccgc
    15901 cgcgacatca accccgccgc gctggccgac ttcccgaacg tcgatggcgt cgtggcgctg
    15961 ccgcactcca cgggttgcgg catgggcgtc aaggggctgt tcatggacac gctgcgacgc
    16021 accctgatcg gctacatgcg ccacgccaac ttccactccg tgctcatggt gggcctgggc
    16081 tgtgaaacca accagatccc cgccctgatg gccaccggcg gcctggaaga aagcagccgt
    16141 ctacagacct tcaccatcca ggacaccggc ggcaccgcca aatcggtggc caagggcatc
    16201 gccaaggtga aggaaatgct gcccgccgcc aatcaggtca cccgcacgcc ggtatcggcc
    16261 tcgcacattg tcgtgggcct gcaatgcggg ggctcggatg gctattccgg catcaccgcc
    16321 aaccccgcgc tgggcgcggc ggtcgatcgc ctggtgcgtg ccggtggcgc ggccatcctg
    16381 tcggaaaccc cggaaatcta cggcgccgaa cacttgctga cacagcgcgc ggtcagccgc
    16441 gaggtgggcg agaaactggt cgagcgcatc aagtggtggg aagactattg ctcgcgcaac
    16501 ttcgccgaac tgaacaacaa cccgtcgccg ggcaacaagg cgggcggact gacgaccatc
    16561 ctggaaaaat ccctcggcgc cgtggccaag gccggcacga ccaacctgaa cgaggtctac
    16621 caatacgccg agccggtcac ggccaaaggc atggtgttca tggattcgcc gggctacgac
    16681 ccggtgtcgg ccaccggcca ggt
//
LOCUS       DASTNK010000068         2009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_973606, whole genome shotgun sequence.
ACCESSION   DASTNK010000068 DASTNK010000000
VERSION     DASTNK010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973606"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..451)
                     /locus_tag="VFR20_05325"
     CDS             complement(35..451)
                     /locus_tag="VFR20_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0229972.1"
                     /translation="MSIGVKKALDAYLVTSIIGDPYSRDCPTRKVLDRIADKWAVLVL
                     GLLMGGPMRFNQLRRAVDGISQKMLSQTLKSLERDGLVSRKAFATVPVTVEYAITPLG
                     QTLADTVDALRVWAETHMEEVTAHQQRYDAGAASGA"
     gene            495..1118
                     /locus_tag="VFR20_05330"
     CDS             495..1118
                     /locus_tag="VFR20_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004565916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0229973.1"
                     /translation="MHVALIGATGNAGSRILAELVRRGHTVTAIVRDATRVPSLPGVE
                     AKSGDVFDKPGIASLLAGHDAVISSVHFAAGDPATLISTVRASGVGRYLVVGGAGGLE
                     VALGEFPLGLKLIDIPNFPEAYRQEAAAGCDFLDLLCAETELDWTFLSPPLSFTPGER
                     TGHFRLGTDLLLSNEHGSSVSFEDYAMALVDELEQPAHSRQRFTIGY"
     gene            complement(1201..1407)
                     /locus_tag="VFR20_05335"
     CDS             complement(1201..1407)
                     /locus_tag="VFR20_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229974.1"
                     /translation="MNDRLNDQAVIQQSYEAVVDKLYGVFNAAYILALANHDDQGKTH
                     AEDAFSSGLLLARQARDRALALLP"
     gene            complement(1500..1703)
                     /locus_tag="VFR20_05340"
     CDS             complement(1500..1703)
                     /locus_tag="VFR20_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229975.1"
                     /translation="MNASTLLWGMLFGSIGLGYFIYGKKQQAMVPLLSGVALMVFPYF
                     VSGTAWLVIIGIALMAVPYFVKV"
BASE COUNT          373 a          676 c          587 g          373 t
ORIGIN      
        1 tcccgcagaa gcggaatcga gccggttttt cgcctcacgc gccggacgcc gcacccgcgt
       61 cgtagcgttg ctgatgggcc gtcacctctt ccatgtgggt ttccgcccag acccgcagtg
      121 cgtccaccgt atcggccagg gtctggccca gcggcgtgat ggcgtattcg accgtgacgg
      181 gcaccgtcgc gaaggccttg cggctgacca ggccatcgcg ctccaggctc ttgagcgtct
      241 gcgaaagcat cttctgggaa atgccgtcga ccgcccggcg caactggttg aagcgcatcg
      301 ggccgcccat caacagacca agcaccagca ccgcccattt gtcggcgatg cgatccagca
      361 ctttccgtgt cggacagtca cgggaatacg ggtcgccaat gatcgaagtg accaggtaag
      421 catcaagtgc cttctttaca ccaatagata tcttggcacc taataacttt tagaaaccac
      481 aagaggaaac cctcatgcat gtcgctctca tcggtgccac cggcaatgcc ggctcgcgca
      541 tcctcgccga actcgtccga cgcgggcata cggtcactgc catcgttcgt gacgcgaccc
      601 gcgtgccttc gctgccgggt gtcgaggcga aatccggtga cgtcttcgac aagcccggca
      661 tcgcatccct gctggcaggc catgatgccg tcatcagttc ggtccatttc gccgcgggcg
      721 atcccgccac gctgatctcg acggtgcgcg catccggcgt aggccgctac ctcgtcgtcg
      781 gtggtgcggg cggtctcgaa gtcgcgctgg gcgaatttcc gctgggcctg aagctgatcg
      841 acatcccgaa cttcccggag gcataccggc aagaagcggc ggcaggctgc gatttcctcg
      901 atctgttgtg cgccgaaacc gaactggact ggacattcct ttcgccgccg ctgagcttca
      961 ctcctggcga gcgaaccggt catttccgcc tcggcaccga cctgctcctg tccaacgagc
     1021 atggcagcag tgtctcgttc gaggattacg cgatggcctt ggtggacgag ctggaacaac
     1081 cagcacactc gcgccagcgc ttcaccatcg gctactgatc gcgcctgtgg aaagggtgcc
     1141 agcttgaccg gcccttcggc cgttgagtgc cgctgggatg gcgaacaagc tcaggcctcc
     1201 ctaaggcagt agcgcgaggg cgcgatccct tgcctggcgg gcaagcaaaa gcccgctact
     1261 gaaggcatct tccgcatggg tcttgccctg gtcatcgtgg tttgccaacg ccaggatgta
     1321 cgccgcattg aagacgccgt acaacttgtc gaccaccgcc tcgtaggact gctggatgac
     1381 agcctggtca ttcaatctgt cgttcatcgt cattctcctg caaggatgat tccggttcgg
     1441 cgctgcgagg ctcgacccgc ttccggcgcc gcgcatgtgc accgctgcgt ggggtctctt
     1501 cagaccttca cgaaatacgg caccgccatc agcgcaatcc caatgatgac cagccacgcc
     1561 gtaccgctca cgaaatacgg gaacaccatc aaggctacgc cgctgagcag cggcaccatc
     1621 gcctgctgct tcttgccata gatgaagtat cccagcccga tcgagccaaa cagcatgccc
     1681 cacaagagtg tcgatgcatt caaggtgcgc cccatggccc gcgggaggaa gcgagcatcg
     1741 tactccggcg accctgtcgt gcctggcgcg agtggagctc cgaacccgct tcccccggcc
     1801 acgggaaagc cccatgcgga aatgacgcaa gcgtaggccc gccgatcgac acccggtggc
     1861 gtcccgcgcc cggcatcacg ccgcgttcgc gggcatcgcc gccatgtcca tccagaacgg
     1921 gccccacacg tggccatcag ggtcggtaag gtcgcggcca tacatgaagt ccatgtcctg
     1981 cacgggattg atgtcagcag tgccaccgt
//
LOCUS       DASTNK010000069        33543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_50029, whole genome shotgun sequence.
ACCESSION   DASTNK010000069 DASTNK010000000
VERSION     DASTNK010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 33543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33543
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50029"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..2076
                     /locus_tag="VFR20_05345"
     CDS             169..2076
                     /locus_tag="VFR20_05345"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEU0229976.1"
                     /translation="MSDTTGEYTRIYKAASADPAGFWAKIAEDIHWTKRWDKVIDDSN
                     KPFYRWFTGGELNTCHNCVDRHVEEGRADQLALIYDSPLAGVTRKITYRELRDEVAQC
                     AGALRDLGVGKGDTVIIYMPMVAEAAIAMLACARIGAVHSVVFGGFAAPELAKRVVDA
                     KPKVVMSASCGLLPGKAVPYKPLLDEALRIAHAEDTPCLVLQRPELECDMIAGRDHDW
                     AEAVSRAAPADCVTLAATDPLYIIYTSGTTGIPKGVVRDNGGHAVALKWSMHAVYGME
                     PGDVYWAASDVGWVVGHSYIVYAPLLQGCTTVLYEGKPVGTPDAGAFWRVCAEHGVNA
                     LFTAPTAFRAIKKEDPQGKLVKGYDLSKFKYLFLAGERSVPDDIAYAQRILGVPVIDN
                     WWQTELGWPALSGCAGIELLPVKPGSATKPVPGYHIDYLDEDGHPQPDGEIGNLVIKL
                     PMPPGTLYTLWNNNERFEKSYLSRYPGYYLSGDAGVRDEEGYHSVMTRIDDIINVAGH
                     RLSTGLMEQVLAAHADVAEGVVVGAADPLKGQVPVGLVVLKSGVERPDADICKELVAS
                     VRHEIGPVADFKSVTVVKRLPKTRSGKVLRVTIRTMADGTELKVPATIEDASVLPEIT
                     DALKLIGYPRK"
     gene            complement(2079..3773)
                     /locus_tag="VFR20_05350"
     CDS             complement(2079..3773)
                     /locus_tag="VFR20_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HEU0229977.1"
                     /translation="MNGGTDRDEVVDGKGGVSGEDTGGPRNPARRRVLGGFAAAGVGL
                     ALGGRDALSAPRPDADARLDATLRSRVRHIVVIYAENRSFSNLYGNFPGVRHPLVEAP
                     ALATVQLDRDGRTPLPQLPKIWGGLVPRPQTVGGRQFAIAQDQITGLRNGPFHLTDAS
                     GAPLPLGVITRDLWHRFYQNQMQINAGRNNQFVAWANAGGLVMGHYRDSAQSLKLWNL
                     AAQYTLCDNFFMSAFGGSWLNHMFLISAQAPHYADAQNSPVRRLLSVLEGEDPAGTRL
                     KVATRSPASALDGPPVFEKDRSLTPDGYAVNTMAAAYQPSHVRPAPGGDPAYADPSNV
                     HVLPPQRYATIGDRLSDKGVAWAWYSGAWQYALDHDGTGPIPDFQYHHQPFNYFANYA
                     PGTEARALHLRDGGMGNDPATNLFIAEIDAGRLPEVAFYKPQSSLNMHAGYADVASGD
                     WHVATIVEHIRRGPQWKDTVIIITHDENGGWWDHVSPPKGDRWGPGSRIPALVISPFA
                     RKGHVDHTLYDTCSIQRFINRVHGLEPLDGVAARDKAFVAGGRVPPGDLTAALDLA"
     gene            3996..4832
                     /locus_tag="VFR20_05355"
     CDS             3996..4832
                     /locus_tag="VFR20_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0229978.1"
                     /translation="MDTVKTLQQLGFTEYEARAYTALVNEGELNGYELAKKTGIPRAN
                     IYAVAEKLVERGAILRADHQSGQRYTPIPPEQLLLGLEVEHKRALVAAAHALAHQANK
                     QEPAAVFNLRGDELLAKARQIVDTCQDSLLVGIQAPEAVALAAALRTAADRGVHITTL
                     CLQACEHECGGCQGDIHRHAIAPRGDRHWLMLVADRHTALVGQTGEGTAEGMTTSQQL
                     VVELAMAFIRQNLALATLGGELGGRIGDLLPPPTLGLLNDLYPDGGFPAYFQNLGAKT
                     LS"
     gene            5012..5401
                     /locus_tag="VFR20_05360"
     CDS             5012..5401
                     /locus_tag="VFR20_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEU0229979.1"
                     /translation="MNTVITRKSVQALLCLSAVAGALFLGGTGQAWAHAHPVQESPAP
                     KSVVTAPNEVRIVYDDPLEPAFSHLNVLTLQGKQVNQAKSSVEGHAHKTMLVALPTLS
                     AGQYQVRWVAVATDGHRTQGHYTFTVK"
     gene            5415..6302
                     /locus_tag="VFR20_05365"
     CDS             5415..6302
                     /locus_tag="VFR20_05365"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEU0229980.1"
                     /translation="MDLATQWLQPLFAAWIDVALIASAGIVVVRGAMPPSVATRLPAL
                     ARRIALLLGVGLVAYLAAATVAMAEPSLVDFPGALWLVLTKTDFGDMGWVALAAWLML
                     MAATCAACRPPVRKRTFPDLLFITGLAAFAYARAATGHAADHGFLSVAVLVHTVHIVS
                     ACAWAGSIGICVLSVSTWRTWTTPQRSKLAHRLSTTATIAVPAVVASGLINVIRALGH
                     SSHPGGSSYLDIAIAKISLVAIAVVLGTWNRQSWMTRLDDGQDAGARGFGRVLLVEAI
                     VLVVVIVLAAKLGTTMIPI"
     gene            complement(6368..7297)
                     /locus_tag="VFR20_05370"
     CDS             complement(6368..7297)
                     /locus_tag="VFR20_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0229981.1"
                     /translation="MLTSMQRLPLNTLPAFRAVADLQNLRAAADRLHLTHSAVSQQIR
                     LLETQLGFPLFDRRGRRVVLNDAGKVLLRSVQTALRQLDEGMQAASGAALGVEHRLRI
                     SVLPSFAQRWLLPRIRRWRDRYPDIHLEIDATQQAVDLSRQGFHAALRQGIGPWEGVE
                     SECLFDGPLSLFAFGCGADAHRLAGREPVDLLGEPLIGGRDLWQSWFAAAGIDTRVAP
                     VAEFNDLGLMLQACEQGLGLVLAPELLAADALVTGSLIRLSPISVVHPDHQSYHLVYP
                     PRLRDWQPLDALRQWLRIEIDISRQEIGRSSAL"
     gene            7410..7709
                     /locus_tag="VFR20_05375"
     CDS             7410..7709
                     /locus_tag="VFR20_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229982.1"
                     /translation="MAAVPCPELPVSPIAASTLHEKNSWRKWWRTLFAASPRTNDKEW
                     RNALPMLLNMDSRLLKDIGAPAWVISEAIYRQRQSANTIEDVLLVRHGRAGARRF"
     gene            complement(7706..8431)
                     /locus_tag="VFR20_05380"
     CDS             complement(7706..8431)
                     /locus_tag="VFR20_05380"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEU0229983.1"
                     /translation="MSDPVIAGPFLGVLGGMGPMASAVFMQRLTALTPAEADQQHVPA
                     ILWSDPRVPDRPAGRAGTGADPLPWMRNGMRHLENAGAGAIVIPCNTAHLWYDALAAS
                     VSVPVLHIVDAVIDDLRRQGLRYGRIGLLGTAATLGLGLYQDRLAKSGYVPVIPDGEV
                     LAGYCAESIRLVKMNRLADAQAPAMEAVARLRDRGVDAVVLGCTELPLALPHAVRGAL
                     DTVLTDSIDALALAAVAWHSALS"
     gene            complement(8449..9450)
                     /locus_tag="VFR20_05385"
     CDS             complement(8449..9450)
                     /locus_tag="VFR20_05385"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HEU0229984.1"
                     /translation="MHLAAFPRVRLGHFPTPLEFMPNLTRHLDGPNLYIKRDDCTGLA
                     TGGNKTRKLEFLVADAISQGADTLITQGAVQSNHARQTVAAAARVGMKCKLLLEQRVP
                     DALPEYEESGNVMLDRLMGGEIVARLPAGTDMQQAMDNLAAELRRAGGKPYVIPGGGS
                     TPVGALGYVACAQELLTQSFDQGVRLDHVVHATGSAGTQAGLVVGLRASNSGVPVYGV
                     SVRAPKPKQEDNVWNLVQRTVEFMRLPPASVERADVVANADYVGKGYGIPTDGMVEAV
                     HLLAQLEGILLDPVYSGKGMAGLIDLVRKGHFKKGENVVFIHTGGAAALFGYRQAFA"
     gene            9571..10467
                     /locus_tag="VFR20_05390"
     CDS             9571..10467
                     /locus_tag="VFR20_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0229985.1"
                     /translation="MKLLWIEDFLALADTGSFSGAAATRYITQPAFSRRIQALEAWLG
                     VELVDRRSQPPRLTDTARRHIPAFRALLRDMNQLRSHMQSEAGGAARLTLVTQHSLTM
                     TRLPALLGRFSRSRAPRVEFTVRSENRDECMTLFMRGDADLLLCMEEPDDPLSRLIPG
                     DRRLSMGEEVLVPLSAPGRNGHPLHAPHAGKPLKLLAFPPNSYLGRVMYKRCFGALFQ
                     TCQVQITHESVFLAGVKEVLLAGLGMAWLPRGLVERELASGSLLMLDESLPRVTLRLA
                     LYRSDHCAYPDTLAHAWALLSR"
     gene            complement(10514..10960)
                     /locus_tag="VFR20_05395"
     CDS             complement(10514..10960)
                     /locus_tag="VFR20_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0229986.1"
                     /translation="MNSKGCSFLVALLVVIVGLGIGYSKFRAQQRKALHDVRIEQWHG
                     PLLAMLGNPVGIRYREQTFSVSGYLCGEFSVASPRQGQGAGDFQRFVVSPNQVVITGA
                     RAYTPSKDDSARRQRQDDQASLDALLKQGATPQQAFNEIAWGPHCR"
     gene            11067..11858
                     /locus_tag="VFR20_05400"
     CDS             11067..11858
                     /locus_tag="VFR20_05400"
                     /inference="COORDINATES: protein motif:HMM:NF022609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HEU0229987.1"
                     /translation="MDDTVLAAMARWPNVPDVFGWLSLSSTGQWRLHPGGAALPEGIQ
                     ANPSASGPDPDPAAMSALSEGEVIGSPAITAFIGRNYGHDTEGRWFFQNGPQRVFVRL
                     DAAPYILRTTGTGTALRTHSGLGISHVLSWVLDDSGRLFAMTDRGPGIVAGRDLPALV
                     NALRTTHGETLAEALALGVFAGGNQPVAGPVEPIHIQPIDALANSPTETAGAGAAKAQ
                     SPVHTAPLRFSTTDGIAATLGFTRFPRERLGPARTPLNRPQEPRP"
     gene            11855..12442
                     /locus_tag="VFR20_05405"
     CDS             11855..12442
                     /locus_tag="VFR20_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU0229988.1"
                     /translation="MTSHAPSFYFPAPRTQKFCTQCGNATTHRVPPDDTRERDVCEHC
                     GAVHYHNPLNVVGVVPIWGEQVLLCRRAIEPRHGMWTLPAGFMELGETTAQGAVREAE
                     EESGAQLELGPLYTVIDVPHVEQVHFFYLARVLSPDLDPGFETLEARFFDTRDIPWDE
                     LAFRTVRTTLEHYLEDRPGGRFPVHHYSLMTPSRG"
     gene            12449..13195
                     /gene="aat"
                     /locus_tag="VFR20_05410"
     CDS             12449..13195
                     /gene="aat"
                     /locus_tag="VFR20_05410"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEU0229989.1"
                     /translation="MLVWLDDASPFPDPATALPDGLVAVGGDLSLARLRDAYTRGIFP
                     WYNEGDPILWWSPDPRMVLACADFQASHSLRKLMRQVARTETAPDATVQIKTDTAFEQ
                     VMRACSQPRAMQTGTWISPAIRAAYLAWHEAGQAHSIETWIDGRLEGGLYGVALGRFF
                     FGESMFHQTRDASKLALAYLVAFLMRRGITHIDCQQQTGHLASLGARPMARDRFRALL
                     DASTSLPAPAWPAGRLAQSGLLASDTLPPS"
     gene            13266..13991
                     /locus_tag="VFR20_05415"
     CDS             13266..13991
                     /locus_tag="VFR20_05415"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEU0229990.1"
                     /translation="MSHPSESIFKTIQLYSTAAYPCSYLSRREARSQVAAPSHMIGSE
                     VYSALVEQGFRRSGLFTYRPHCDTCRACQPIRVDVDRFQPSRTQRKTGQHHRSLAAQA
                     SDLHWDPEHFDLYNRYQKRRHPGAGMDEDSQAQYTQFLLTSRVDSRLVEFRTPEGSLV
                     MVSIIDILKTGLSAVYTFFDPDLPGSLGTYGILWQIGQCRRLGLPWLYLGYWIEESRK
                     MRYKSQFRPFQILQDGAWIEPTP"
     gene            14039..15079
                     /locus_tag="VFR20_05420"
     CDS             14039..15079
                     /locus_tag="VFR20_05420"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEU0229991.1"
                     /translation="MPVLFNAYSLARPALFSLDAETAHELTLSSLAKAYDCPLTRRLL
                     HTSPELARPLMGMKLRNPVGLAAGLDKNGAFIDSLGNLGFGFIEVGTVTPRAQPGNPK
                     PRMFRLPRAQALINRLGFNNRGLAEFIENVKKSRWRQNGGILGLNIGKNADTPMDRAV
                     DDYLHGLEGVYAHADYVTINISSPNTQNLRSLHGRDELTALLGALHDRRQMLADRHGR
                     HVPLAVKIAPDLTVEQIDAIADTLPRYAIDGVIATNTTLSRDAVRGLAHAGETGGLSG
                     PPVHQMSLAVIRHLRQRLGDSIAIIGVGGIMSGQDAREKIDAGADAVQIYTGLIYRGP
                     ALIPECVRALGA"
     gene            complement(15144..16943)
                     /locus_tag="VFR20_05425"
     CDS             complement(15144..16943)
                     /locus_tag="VFR20_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEU0229992.1"
                     /translation="MFTTRPEITGTFGVTTSTHWLATAVGMSLLERGGNAFDACVAAA
                     FTLQVVEPNLVGPAGEAPAIFHSVSTGKIEVLCGQGTTPADATLDHYRAQGLSLIPGN
                     GLLSTVVPGAFDAWMILLRDHGTRSLRDVLEPAIHYAEHGHPVLERVSDTIAVLQEFF
                     ETYWPSSAAVYLPNGSAPQPGTLFRNPALARTWIRLLAEAESVGGDRERQIEKARDVF
                     YRGFVAEAIDKFVRGTEAMDESGSPHRGVLSGADMAAWSASYEAPVRYRYGDITVSKA
                     GPWSQGPAFLQTLALLRHTDIAELKRGDPRFVHLLMEAMKLAFADREAYYGDPDFVDV
                     PLAHLLSDDYSRARAALIGEQASTLLRPGVVPGYEAQAGRVNAALDRLSSLKPDGVAT
                     SALERIGRMVSARKGDTTHVDVIDRWGNMISATPSGGWLQSSPVVPGLGFGLNTRAQM
                     FWLEEGLPCTLQPRKRPRTTLSPTLLQRNGEPWMAFGTPGGDQQEQWQLQLFLRHVHQ
                     GMNLQEAIDAPMFYTQHFPSSFYPRDRRPASLTIERDFGADALNDLKRRGHVVEESPE
                     WSIGRLTAASRDPDGLLHAAASPRLVQAYAAGR"
     gene            complement(17001..18500)
                     /locus_tag="VFR20_05430"
     CDS             complement(17001..18500)
                     /locus_tag="VFR20_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0229993.1"
                     /translation="MNKRGMTLTAIAAILISGAACAQSTLKIGLQDDPDQLDPVRART
                     FVGRIVFASLCDKLVDITPDLKFVPQLATSWSWLDNKTLKFTLRDKALYQDGTPIDAA
                     SVKANLDRARTLPGSNRKSELASVASVDAPDPHTVILHLSEPDASLLSQLSDRAGMMQ
                     SPKSFNKDPGKHPVCSGPYKFKERVQNDRIVLTKFKRYWDAKDYHFDTVIFKPIPDST
                     VRLNNLRAGDLNIAGRLAPSDAQAVRSDPTLDFRPVTGLGFQAISVNFAHGPRGESNP
                     LAKDPRVRQAFDLAIDRNAINQVVGMGLYQPAFQPFPPASFAYDKNIGRKGADIPKAK
                     ALLKAAGLKRVAVQISYGNNTIMQQVMELIQATEREAGFDVTLRPMEFAALQSTLASG
                     DFQLGQAGWSGRVDPSGNVYQYVSCKGSLNDGKFCDPRLDALLNEARQETDEGKRKML
                     YAKFLQGMQKEHPIFYLFYLPWTFGVQKRVHGFVPYPDGLIRLKGVTLQ"
     gene            complement(18540..20399)
                     /locus_tag="VFR20_05435"
     CDS             complement(18540..20399)
                     /locus_tag="VFR20_05435"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEU0229994.1"
                     /translation="MNAERVIQVENLSVSFKTPEREVHAVRDVSFHLNRGETLAIVGE
                     SGSGKSVTSLALMRLVEFGRGRITQGGLLLRRRNGDVIDVARAEESVMRTVRGADIAM
                     IFQEPMTSLNPSFTVGWQITEALRLHQGMDARAAQAETLRLLETVRIPDARALCSRYP
                     HQLSGGMRQRVMIAMALSCRPQVLIADEPTTALDVTIQAQILELIRELQRDMDMGVIF
                     ITHDMGVVAEVADRVMVMYHGEKVEEGPSEPIFLTPRHWYTRALLSAVPRLGSMKGLD
                     QPAPFELLREGATRDGQASTPGLRPVTTTARYGEAPVLRVQGLTTRFDIADGFFGQVR
                     RRVHAVEQVSFDLFGGETLALVGESGCGKTTTGRSLALLGTPRAGRILYEGQDIGALS
                     GSRMQALRRNIQFIFQDPFASLDPRATVGYSIMEPLLIHRVASGRKAQERVAWLMEKC
                     GLSPEMADRYPHEFSGGQRQRICIARALALDPRVIIADESVSALDVSIQAQIVNLLLD
                     LQRELGLSILFISHDMAVVERISHRVVVMYLGQVVEIGPRRAVFENPQHPYTRKLMAA
                     VPIADPSRRHREHSLLVDEIPSPVRKVGDEPRVDPLVQVGEGHFVARHSVGAY"
     gene            complement(20411..21292)
                     /locus_tag="VFR20_05440"
     CDS             complement(20411..21292)
                     /locus_tag="VFR20_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0229995.1"
                     /translation="MTASTVNAPVTGAAPRRRSRAWAKFRRNRSAMLGAIIILFFVVL
                     ALLAPLIAGQDPLHVSFTAIRKAPSLAHWFGTDELGRDIFSRMVYGARASLLAGMCSV
                     LIALLVGVPLGLLSGYFGGLIDGCISRVTEALFSIPFLILAIALAAFLGPSLINAMIA
                     IGVSAAPLFIRLARGQVLAVKNEDYVQSTRSLGASDRRIILRHVLPNILPALIVQATI
                     QIAKAIIAEASLSFLGLGLQPPDPSWGSMLNTAKNFMIQAPWMSVFPGCAIFLVVFGF
                     NLLGDGLRDALDPRQED"
     gene            complement(21289..22233)
                     /locus_tag="VFR20_05445"
     CDS             complement(21289..22233)
                     /locus_tag="VFR20_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0229996.1"
                     /translation="MFKLIVRRMIVAIPTLILVSMMIFSLQKILPGDPVLAMAGEERD
                     PAVLNYLREKYHLNDPLPQQYLAWIKRVAHGDLGKSLRTDIPVTTLVAQKLPVTIQLS
                     VMAMLFALVTGIPLGILAAVKKGTRVEFGANLFALSGMSIPNFWLGILLIMLVSVHWR
                     LLPASGFVPPSQDFWLSIKTSLMPSLVLAAVISAYLMRHTRSAMLEALSADYVRTARA
                     KGASEWSVVLRHAFRNALMPIVTLVTILFGELLAGAVLTEQIFGIPGFGKLLVDAVFN
                     RDYAVVQGVVLCIATGFIVLTLLADVLYILVNPRLRNS"
     gene            complement(22311..23825)
                     /locus_tag="VFR20_05450"
     CDS             complement(22311..23825)
                     /locus_tag="VFR20_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0229997.1"
                     /translation="MKKIVLVAGGILLGAATACSVAQTLRIGLQEDPDTLDPARARTY
                     VSRVVFASLCDKLVDINAKLQFVPKLAKSWSWSPDNKTLTMKLRDDALFQDGTRFDAA
                     AAKANLERDMTLKGSQRRSELASIQDIEAPDPTTLIIKVKQPDATLLAHLSDRAGMML
                     SPKSFASGDLANVARHPVCSGPYQFVSRVQNDRIVLKKFEKYYDAKDYHFDKVVFLPI
                     PDTTVRLQNLRSGGIDILGRLNPSDIKTVKSDPSLEFKPVTGLGFQQFMFNVGNGPRA
                     KTNPFANKLVRQAFQLTIDRDAINQVIGNGIFTPAQQPFPLASPYHSDKFPVVKPDIA
                     KAKALLKKAGMPVVKAELSFGNNTISSTTAEMVQAMAAQAGFQLSLRPTEYAAMLADD
                     SQGKFQIDLRGWSGRVDPDGNIFNFVTCKGSLNDGKYCNPEVDKLLKEARTVPDVASR
                     KAIYDKAQAILQDDLPSLYIYYMPWPFAIRKQVHGFTPYPDGMIRLRGVTLAKN"
     gene            complement(23874..25499)
                     /gene="ggt"
                     /locus_tag="VFR20_05455"
     CDS             complement(23874..25499)
                     /gene="ggt"
                     /locus_tag="VFR20_05455"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU0229998.1"
                     /translation="MKPFDWTNPYPSTRTPVFARNVVSTSHPLAAQAGLRMLLKGGNA
                     VDAALAAAATLVQVEPVSNGLGGDCFALVWDGKRLQGLNSSGVAPAAWNVDYFKRKYG
                     VDGNGLATRPTRGWDAVTVPGVVAGWAALHEKFGKLPFEDLFEPAIEVAERGYAVPPV
                     VAGKWAKAVDELRNQPGYAQAFLPHGRAPHVGELFRLPGVARTLRRIAETRGRDIYKG
                     ELAEKIVAFSREGGGSLTLDDLRNYRPEWVEPIAKNYRDYTVHEIPPNGQGIAALMAL
                     GIVEQFDLQSMPVDSVTSQHVQIEAMKLAFADLYRYVSDPRSMDVASAQLLDDDYLRG
                     RAKLIKLDRAGSFQAGRPHAGGTIYLSAADESGMMISFIQSNYMGFGSGVVVPDTGIS
                     MQNRGMGFSLDPRSPNVVEGGKRPFHTIIPGFLTRGGKPVMSFGVMGGDMQPQGHLQT
                     IVRMVDYHQQPQAACCAPRWKVNTDFSLDIEPNMNPDTVSGLKDLGHVLKSVNDPYMD
                     FGAGQFVWKLSDDPNHGYVAASDSRRDGQAVGF"
     gene            25615..26499
                     /locus_tag="VFR20_05460"
     CDS             25615..26499
                     /locus_tag="VFR20_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0229999.1"
                     /translation="MGSLRFLRTFVAVARRGSFSDAAEQVALTQAAVSLQMRALETEF
                     GRTLFDRSGRLASLNEAGRELLPQAEQLLEIYDRMRTPRPAPGTFAGTLSIGAIVSCM
                     GTLARVVSQLKIEHQELIIRLSSGKSRELCRKVENGEIDAALVVGAGRPPPRTRWKRL
                     YQEPVCLVVSSQVPGDDPRQLLRSHPFLRFDRSQHTGAQIDRVLRKMGIVPDDFLELN
                     ALEQLLDLVRHDVGITILPLLHTMRWNDGNGLRAIPLPAELGAITREIGMLERRDHNL
                     HAAAAMIFERYAALSAKG"
     gene            complement(26792..27370)
                     /gene="phaP"
                     /locus_tag="VFR20_05465"
     CDS             complement(26792..27370)
                     /gene="phaP"
                     /locus_tag="VFR20_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525805.1"
                     /note="Members of this family are phasins (small proteins
                     associated with inclusions such as PHA granules). Note
                     that several different families of phasins have been named
                     PhaP despite very little sequence similarity to each
                     other; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01841 family phasin"
                     /protein_id="HEU0230000.1"
                     /translation="MSAIPQHILASQKASLDNLVAIQNSFFEGFEKLVDLNLKVVKAS
                     LEEAAQKTHEVIGLKDAQEAIAFASGLTQPNAEKALAYGKHVYDIVSGVQADLTKLGE
                     EQISQNQQQVSEAIEQLSKNAPAGSESAVALLKSSLSTATSAYESVAKAAKQASKTAE
                     SNIAAATNATLKAASDVAEAAKPAPRARRAAA"
     gene            complement(27573..28403)
                     /locus_tag="VFR20_05470"
     CDS             complement(27573..28403)
                     /locus_tag="VFR20_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU0230001.1"
                     /translation="MSRTPSLSYSSGPLSPVNSSAVTVQVLERAMLLLDVLADHVEPV
                     TLKDLAAATHLHTSTTHRILNDLVVGRYVERVDSGLYRLGMRLLELGSLVKGRLDVRE
                     VAMEPMRELHRLTGQTVNLSLRQGDEIVYIERAWSERSGMQVVRAIGGRAPLHLTSTG
                     KLFLSISDPRQVRAYAMRTGLAGTTRNSITKLDDLERDLALVRRHGYSRDNEELELGV
                     RCIAAGVYDDTGKLLAGLSLSAPAERMRDEWIQALVKTASHISGALGYEGRPVSDRVS
                     "
     gene            complement(28633..29436)
                     /locus_tag="VFR20_05475"
     CDS             complement(28633..29436)
                     /locus_tag="VFR20_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014906131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEU0230002.1"
                     /translation="MSTVASQVKLLIIGDEILSGRRQDKHFSKAIELLSQRGMQLAGA
                     EFVPDDRDTIAAVLTRTFASGGVVFCCGGIGATPDDQTRQAAAQALGLELELHPEAAR
                     LIAERCADNARRGSGSADMSLPENQQRLQMGVFPKGAEIVPNPFNKIPGFFIHNHTFM
                     PGFPEMAWPMMEWTLDTRYAGSQHKVTRVEHSFLAFRLPESRITPALEELERKWPGVK
                     AFSLPSMGGSGHPHIDLGVKGEPDAAAQALAYLRDEVVRVGGSLTPPEK"
     gene            complement(29499..30296)
                     /locus_tag="VFR20_05480"
     CDS             complement(29499..30296)
                     /locus_tag="VFR20_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HEU0230003.1"
                     /translation="MSNFDLVARPAGAPPATGLAAVGLAFRRALVSQCHPRMILALFL
                     PFIIALLGAILLLWIFWTPLTHWLGGESASWTYLNDVDQWLLALGLFSLKLYLIPFLA
                     LCILLPLSGILGLVIAAIFVMPIVLNHLEHREYKGLRRQGEFAGTVSAWNAAWVGGLF
                     IIGWALTMPLWLIPPLAVVLPVFWWAFAFTRMLRVDAIVEHASARERRIVWRRHNRQL
                     WLIGSCLAVLNLFPPAWLVLPVFSALVFAHFSLEALRQLRRETVLEA"
     gene            30360..30755
                     /locus_tag="VFR20_05485"
     CDS             30360..30755
                     /locus_tag="VFR20_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU0230004.1"
                     /translation="MTVFNPLPDLSALRARLVESHELIVACYCAQWCDTCRAYRADFE
                     NLAAQWPRHVFVWIDIEDSPDLLGDVDVENFPTIALQDARRTVFFGTLLPYASHLDRL
                     IGRSDSLTDTPTEAPPLLGPLLVTETAGK"
     gene            complement(30764..32137)
                     /locus_tag="VFR20_05490"
     CDS             complement(30764..32137)
                     /locus_tag="VFR20_05490"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU0230005.1"
                     /translation="MTDSTHTDTTTTFSDFGLHPGILQAVTAAGYVTPTPIQAQAIPR
                     VMDGCDVMGAAQTGTGKTAAFTLPILHRLMPLATHSASPARHPVRALILTPTRELADQ
                     VYQNVANYSKATPLRSTVVFGGVDIRPQRDALRRGCEILIATPGRLLDHVEQKNVSLA
                     QVEILVLDEADRMLDMGFLPDLDRIMRLLPAKRQGLLFSATFSNEIRKLARSFLHDPV
                     EIEVAARNATAETVSQIAYPVSSADKRAAVVHLVKSRGLSQVIVFSNTKIGTSRLARQ
                     LERDGVKAESIHGDKSQLDRMKALDAFKAGQLDVLVATDVAARGLDVAGVPCVINYDL
                     PFNAEDYVHRIGRTGRAGASGEAIAFFAPDEERFLIDIEKLIKSKIPRGQLELPIEAY
                     RPPQGQGGAPRGRSGGRPTRPVDDFFHKPYVPSPAPASAVLNAPTSHHTHAHKRSVGV
                     LLGGRGR"
     gene            32374..33294
                     /locus_tag="VFR20_05495"
     CDS             32374..33294
                     /locus_tag="VFR20_05495"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEU0230006.1"
                     /translation="MSMSDRDGFIWYDGKLVPWRDATTHVLTHSLHYGLAVFEGVRAY
                     KTNQGTAIFRLHDHTKRLFNSAHIYQMPIPYDRDTIMRAQCEVVRGNQLESCYIRPLV
                     FYGSEKMGVSPKGASVHVAIAAWPWGAYLGEEALKQGIRVKVSSYARQHVNVTMPRAK
                     VASTYANSILANAEALDHGYDEAILLDTEGFLAEGSGENIFLVKDGVLCEPEIASALT
                     GITRSTIHALAADLGIPVQTKRLTRDDLYIADEAFFTGTAAEVTPIREVDGRVIGPGQ
                     RGPITEKLQQAFFDLVSGKNAKYQQWLTPV"
     gene            33353..>33543
                     /locus_tag="VFR20_05500"
     CDS             33353..>33543
                     /locus_tag="VFR20_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEU0230007.1"
                     /translation="MSSTTATRHDIIYVEAKDLPVYCPGPKAPLWSMHPRVYIEVAKT
                     GTAVCPYCSATYQLKEGEK"
BASE COUNT         6417 a        10839 c        10361 g         5926 t
ORIGIN      
        1 gacgaagacc cctttgctgt ctaaaaggcc ttcgtctggt ggcacagaac atctggtagc
       61 gtgtcacttc cggtaaatcg ttattgaaga ctacgacgag taatgagtac agtgtgggcg
      121 atggctgctc acaaggaaaa gatagcgcac atataagaag gagacactat gagcgacacc
      181 accggcgaat acaccaggat ctacaaagcc gcctcggccg atccagccgg cttctgggcg
      241 aagatcgccg aggacatcca ctggacaaag cgctgggaca aggtcatcga cgactcgaac
      301 aagcccttct accgctggtt cacgggcgga gagctgaaca cctgccacaa ctgcgtcgat
      361 cgccacgtcg aggaaggccg cgccgatcaa ctggcgttga tctacgacag cccgctggcc
      421 ggcgtcacgc gcaaaatcac ctaccgcgaa cttcgcgacg aagtcgccca atgcgccggg
      481 gccctgcgtg acctgggtgt gggcaaaggc gacacggtca tcatctacat gccgatggtg
      541 gccgaggccg ccatcgcaat gctggcgtgc gcgcgcatcg gcgccgtgca ctccgtggtg
      601 ttcggcggct tcgcggcgcc ggagctggcc aagcgcgtgg tcgacgccaa gccaaaagtc
      661 gtgatgtccg cctcgtgcgg cctgttgcct ggaaaggccg tgccctacaa gccgttgctg
      721 gacgaagcct tgaggatcgc gcacgccgag gacacgccgt gcctggtgct gcaacgcccg
      781 gagctcgagt gcgacatgat cgccggccga gaccatgact gggccgaggc ggtcagccgg
      841 gcggctcccg ccgactgcgt cacactcgcc gccaccgacc cgctctacat catctatacg
      901 tccggcacga ccggcattcc gaaaggggtg gtgcgcgaca atggtggcca tgccgtggcg
      961 ctgaagtggt cgatgcatgc cgtgtatggc atggaacccg gcgatgtcta ttgggcggcc
     1021 tcggacgtgg gctgggtggt gggtcattcc tacattgtct atgcaccgct gctgcaaggc
     1081 tgcacgaccg ttctgtacga aggcaaaccc gtgggcacgc ccgatgccgg ggccttctgg
     1141 cgtgtttgcg ccgaacatgg tgtcaatgcg ctgttcacgg cgccgaccgc ttttcgcgcc
     1201 atcaagaaag aggacccaca gggcaagctg gtcaagggct acgatctgtc gaagttcaag
     1261 taccttttcc tcgctggcga gcgcagtgtt cccgacgata tcgcctacgc gcagcgcatc
     1321 ctgggtgtgc cggtcatcga caactggtgg cagacggaac tcggctggcc tgccctgagc
     1381 gggtgcgcgg gtatcgagct gctgccggtc aagcctggct cggcgaccaa gcctgtgccc
     1441 ggctaccaca tcgactatct cgacgaagac ggccacccgc agcccgatgg cgaaatcggc
     1501 aatctcgtca tcaaactgcc catgccgcca ggaacgctct atacgctgtg gaacaacaat
     1561 gagcgtttcg agaagagcta cctgtcgcgt tatcccggct actacctctc gggcgacgcg
     1621 ggtgtccggg acgaggaggg gtaccattcg gtcatgacgc gcatcgacga catcatcaat
     1681 gtcgccgggc accgcctgtc gaccggcctg atggaacagg tgctggccgc gcacgccgat
     1741 gtcgccgagg gcgtggtggt gggcgccgcc gacccgctca agggacaggt tccggtgggg
     1801 ctggtggtgt tgaaatccgg cgtcgaacgg cccgacgccg acatctgtaa ggaactggtc
     1861 gccagcgtcc gtcatgaaat cggccccgtg gcggacttca agagcgtgac agtcgtgaaa
     1921 cgcctgccca agacccgttc cgggaaagtg ttgcgcgtga cgatccgcac catggctgac
     1981 ggcacggaat tgaaagtgcc ggcgaccatc gaggacgcgt cggtcctgcc ggagatcacc
     2041 gacgcgctca agctgatcgg ctatccacga aaatagacct aagccagatc cagcgccgcc
     2101 gtgagatccc ccggcggcac ccgcccgccg gccacgaaag ccttgtcccg cgccgccacg
     2161 ccgtccagcg gttccaatcc gtgcacacga ttgatgaacc gctggatcga gcaggtgtcg
     2221 tacaaggtgt gatcgacatg ccccttccgg gcgaatggcg agatcaccaa cgccgggatc
     2281 cggctgcccg gcccccagcg atcacccttt gggggcgaca cgtgatccca ccacccgccg
     2341 ttttcgtcat gcgtgatgat gatgacggta tccttccatt gagggccgcg ccggatgtgc
     2401 tcgacgatcg tggcgacgtg ccagtcgccc gaggccacgt cggcatagcc cgcatgcatg
     2461 ttcaggctgc tctgaggctt gtagaacgcc acctcgggca agcggccggc atcgatctcg
     2521 gcgatgaaca ggttcgtggc cgggtcgttg cccatgccgc catcgcgcag atgcaatgcc
     2581 cgcgcctccg tgcccggcgc atagttggcg aaatagttga agggctgatg gtggtactga
     2641 aaatcgggta tgggtcccgt gccatcatgg tccagcgcat actgccaggc gccgctgtac
     2701 caggcccatg ccacaccctt gtcggacagc cggtcaccga tggtcgcgta gcgctgcggc
     2761 ggcaagacgt gcacattgga cgggtcggca taggccggat cgccccccgg cgcggggcgc
     2821 acatggctgg gttggtaggc cgctgccatg gtgttgacgg catagccgtc aggcgtcagc
     2881 gaccggtctt tctcgaacac cggcgggccg tcgagcgccg aggcgggtga tcgggtcgcc
     2941 accttcagcc gcgtaccggc cggatcctcc ccttcaagaa cggacaacag tctgcgcacg
     3001 gggctgttct gcgcatccgc gtaatgcggc gcctgtgccg agatcaggaa catgtgattc
     3061 agccacgacc ctccgaacgc cgacatgaag aagttgtcgc acaaggtgta ctgtgcggcc
     3121 aggttccaca acttgagcga ctgggcggaa tcccggtagt gtcccatgac cagtccgcct
     3181 gcattcgccc atgcgacgaa ctggttgttg cgcccggcgt tgatctgcat ctggttctga
     3241 tagaagcggt gccatagatc gcgggtgatc acgcccagcg gaagcggcgc gccactggca
     3301 tcggtcagat ggaacgggcc attgcgcaaa ccggtgatct gatcctgcgc aatggcgaat
     3361 tgcctgccgc ccacggtctg ggggcgtggc accagaccgc cccatatttt gggcaattgg
     3421 ggcaacggtg tccggccatc gcggtcgagc tggactgtgg ccaacgcagg cgcctcgaca
     3481 agcgggtgcc gcacgcctgg gaaattcccg tacagattac tgaagctgcg gttctccgcg
     3541 tagatgacca cgatgtggcg cacgcgtgag cgcaacgtgg cgtcaaggcg ggcatccgca
     3601 tcggggcgcg gcgccgacaa cgcatcccgc ccgcccaggg ccagaccgac tccggctgcg
     3661 gcgaaaccgc ccaggacgcg gcgtcgggcg ggattgcgcg ggcccccggt gtcctcgccg
     3721 gacacccctc ctttaccatc gaccacctcg tcccgatctg ttcctccatt catggccttc
     3781 gtcctccttg agacgtgggt tgcgtacacc gccccgatac gaccttgccc agcaagcgta
     3841 gcgctttcac atgacaaatg gttgaagccc tgattgatcc ggaaaaccat tgcgcgtctt
     3901 gacagtccgc caattctgta tatagtagct attaaaacaa ctactaataa acacctcaat
     3961 tcaaccattt tgcgaatccg catgacccgc acgccatgga cactgtcaag actctccaac
     4021 aactgggatt caccgaatac gaggcacgcg cctacacggc gctggtcaac gagggcgagt
     4081 tgaacggtta cgaactggcc aagaaaaccg gcattccccg ggccaacatc tatgccgtgg
     4141 ccgagaaact ggtggaacgc ggtgccattc tgcgcgcgga ccaccagagc gggcagcgct
     4201 acaccccgat tccgccggaa caattgttgc tcgggctgga agtcgaacac aagcgggccc
     4261 tggtcgccgc cgcgcacgcc ctggcgcatc aggccaacaa gcaagagccc gccgctgttt
     4321 tcaatttgcg cggcgatgaa ctgctggcca aggccaggca aatcgtggac acctgccagg
     4381 attccctgct ggtgggcatt caagcgcccg aagcggtcgc tctggcggcc gccctgcgca
     4441 cggcggccga ccgcggcgtg cacatcacca cactgtgcct gcaggcctgt gaacacgaat
     4501 gcggtggatg tcagggcgac atccaccgcc atgccatcgc gccgcgcggc gaccgccact
     4561 ggctgatgct ggtcgccgat cgtcataccg cgctggtcgg gcaaaccggc gaaggtaccg
     4621 cggagggcat gaccaccagt cagcaactgg tggtcgagct ggccatggcg ttcatccgcc
     4681 agaacctcgc cctggcaacg ttggggggtg aactgggcgg ccgcatcgga gacctcctgc
     4741 ccccccccac actgggtctg ctgaacgatc tgtaccccga cggggggttt cccgcctatt
     4801 ttcagaacct cggcgccaag acactttcct gacgtcgata tttccgacct catgctcgaa
     4861 gagcatggtc gacatcaacg taaaatatag gctccacgcg gctgaaaaca cgcaacgcct
     4921 gctcaaccaa gcgatgcaca acgaggccca tgcgttaggc gagcggcaag tcaagttggc
     4981 atcgcttcac ttcactaagg tcaccatgtt catgaacacc gttatcacac gaaaatccgt
     5041 ccaagcattg ttgtgcctgt cagccgtggc tggcgcgctt ttcctgggcg gcacgggcca
     5101 ggcgtgggca catgcgcacc ctgttcaaga atcgcccgcg cccaagtcgg tcgtcacggc
     5161 ccccaatgaa gtccgcatcg tgtatgacga cccgcttgaa cccgcgttca gccacctgaa
     5221 cgttctcacg ctccagggca aacaggtcaa ccaggcgaaa tccagcgtcg aagggcatgc
     5281 gcacaagacc atgcttgtcg cgttgcccac tctgtcggcc ggacagtatc aggtgcgttg
     5341 ggtcgccgtg gccaccgacg gccatcgcac acagggtcat tacaccttca ccgtgaagta
     5401 gccccagggt gtcaatggat ctggccacgc aatggttgca gccgctgttc gccgcctgga
     5461 tcgacgtggc gctgatcgcc tccgcgggaa tcgtggtggt gcgtggcgcc atgccgccct
     5521 ccgtggcgac acgtctgcca gcgctggctc gccggatcgc cttactgctt ggcgtgggtc
     5581 tggtggcgta tctcgccgcc gccacggtgg cgatggccga gccaagcctc gtcgatttcc
     5641 cgggcgcgct ctggctggtg ctgaccaaga cagacttcgg ggacatgggc tgggtggcat
     5701 tggccgcatg gctgatgctg atggcagcga cctgcgcggc atgccggcct cctgttagaa
     5761 agcgcacgtt tccggacctg ctgtttataa ccggcctggc ggcgttcgcc tatgcccgcg
     5821 ccgccaccgg acacgcggcc gaccacggct tcctcagcgt ggccgttctg gtgcataccg
     5881 ttcacatcgt ctccgcctgc gcttgggcgg ggtcgatcgg catctgcgtg ctatcggtat
     5941 ccacttggcg cacctggaca acaccgcagc gcagcaaatt ggcgcatcgg ttgtcgacga
     6001 ctgccaccat cgccgtaccc gccgtcgtag ccagcggctt gatcaacgtc atccgggccc
     6061 tgggccattc cagccatccg ggcggctcgt cgtacctgga catagcaatc gcaaagatct
     6121 cactcgtggc catcgccgtg gtgctgggca catggaatcg ccagtcctgg atgacacgcc
     6181 tggacgatgg ccaggacgcg ggcgcgcggg gattcggcag ggtgttgctt gtcgaagcca
     6241 tcgtgctggt cgtggtgatc gtcctggccg ccaagctcgg cacgaccatg attccgatat
     6301 aaggcagcgg ccacgcttcc ccgcattccg ggggcattct ttgcgggagt ggaacgccca
     6361 cccggtttca cagcgccgag gaccgcccga tttcctgacg agagatgtca atttcaatgc
     6421 gcagccactg gcgcaaggcg tccagcggct gccagtcgcg caagcgcggc ggatacacca
     6481 gatggtatga ctgatgatct gggtggacaa ccgaaatcgg cgacagcctg atgagagacc
     6541 ccgtcaccag ggcatcagcg gccagcaatt ccggagccaa caccaagccc agcccctgct
     6601 cgcaggcttg cagcatcagg cccaggtcat tgaattcggc gaccggcgcg acgcgggtgt
     6661 caatgccggc ggcggcgaac cacgactgcc atagatcgcg cccaccgatc aacggttcac
     6721 ccagcagatc gacgggttct cgtcccgcca gacgatgggc gtcggcacca cagccaaacg
     6781 cgaacagaga caatgggcca tcaaaaagac attcggattc cacgccctcc cagggcccga
     6841 ttccctggcg caaggccgcg tgaaacccct gccgactcaa atcgactgcc tgctgggtgg
     6901 cgtcgatttc cagatgaatg tcgggatagc gatcacgcca gcgccggata cgcggcaaca
     6961 accaacgctg cgcaaacgac gggagaacag agatacgcaa acgatgctcg acccccaggg
     7021 ccgcccccga ggctgcctgc atgccttcgt caagctggcg caatgcggtc tgcacgctgc
     7081 gcagcagtac cttgccagcg tcattgagca cgacccgccg cccccgccta tcgaacagag
     7141 gaaaacccaa ctgggtttca aggaggcgga tctgctggct gacagcgcta tgcgtgaggt
     7201 gcagacggtc ggctgccgcg cgcagattct gcagatcggc gacagcccgg aacgcaggca
     7261 gggtattaag aggaagacgt tgcatgctgg taagtatagc ttaccagcgt ggcaagaatt
     7321 cgttgatttc ttcgcaagaa gtggttaatt aaaataacca atgtagtggt tattacctat
     7381 atttgcgttg cacttgaaga gatcctgtca tggctgccgt accttgccca gaattgcccg
     7441 tttcgcccat cgccgcatca acgttgcatg agaaaaactc gtggcggaaa tggtggcgta
     7501 ccttgttcgc cgccagcccc cgcacgaacg acaaggaatg gcgcaacgcc ctgcctatgt
     7561 tgctgaacat ggacagccgc ctgctgaagg acatcggcgc accggcctgg gtgatctccg
     7621 aggctatcta ccgtcagcgg caatccgcca acaccatcga agacgtcctt ctggtgcgac
     7681 acggccgcgc gggcgcacgt cgtttttaag acagcgcgga atgccaggcc accgcagcca
     7741 gcgccagcgc gtcgatcgag tccgtcagca ctgtgtccag tgccccgcga accgcatgag
     7801 gcaaggccag cgggagttcg gtgcatccca gcaccaccgc atcgacacca cggtcacgca
     7861 gccgagccac cgcctccatg gccggggctt gcgcatcagc caggcgattc atcttcacga
     7921 gccggatgga ttcggcgcag tagcccgcga gcacctcgcc atcgggaatg accggcacat
     7981 aaccgctttt cgccagcctg tcttggtata gccccaggcc cagtgtggcc gccgttccca
     8041 acaacccgat ccgtccgtac cgcaaaccct gccgccgcaa gtcgtcgatg acggcatcga
     8101 cgatatgcag cacgggaacc gacaccgagg cggccagcgc gtcataccac aaatgcgccg
     8161 tgttgcacgg aatgacgatc gcacccgcgc cagcgttttc gagatgccgc atgccattcc
     8221 gcatccacgg caacgggtcg gctccggtgc ccgcccgtcc ggccgggcgg tccggcacgc
     8281 gcgggtcgct ccacaggatg gctggcacat gctgctgatc ggcctcggcc ggcgtcagcg
     8341 ccgtgaggcg ctgcatgaag accgcgcttg ccatggggcc catgccgccc agcacaccca
     8401 ggaatggccc ggcaatgact ggatcactca ttgggcttcc cggggcgatt acgcgaaagc
     8461 ctgccgatag ccgaacaggg cggccgcgcc gccggtgtga atgaatacga cgttctcacc
     8521 cttcttgaaa tggcctttgc ggaccagatc gatcagaccg gccatgccct tgcccgaata
     8581 cacgggatcc agcaatatgc cctccagctg ggccaaaaga tggacggctt ccaccatgcc
     8641 atccgtggga atgccatagc ccttgccgac gtagtcggcg ttggcaacca catccgcgcg
     8701 ttcgaccgac gcgggcggca ggcgcatgaa ctcgactgtg cgctggacca gattccagac
     8761 gttgtcttcc tgcttgggct tgggtgcgcg cacgctcacg ccatagacgg gcacgcccga
     8821 attggaggcg cgcaaaccca ccaccaggcc tgcctgcgta cccgcgctgc cggtcgcgtg
     8881 caccacatga tcgagacgga caccctggtc gaacgattgc gtcaggagtt cctgggcgca
     8941 cgccacgtaa cccagcgccc ccaccggggt ggagccgccc ccggggatga catagggttt
     9001 gccgccggcg cggcgcaact cggccgcgag attgtccatg gcttgctgca tgtcggtgcc
     9061 agccggcagg cgcgccacga tctcgccgcc catcaggcga tcgagcatga cgttgccgga
     9121 ctcttcatat tcgggcagcg cgtcgggcac acgctgttcc agcagcagtt tgcatttcat
     9181 gccgacgcgc gccgcggccg ccaccgtctg gcgggcgtgg ttcgattgca cggcaccctg
     9241 tgtgatcaac gtgtcggcgc cctggctgat cgcgtccgcc accaggaatt cgagcttgcg
     9301 tgtcttgttg cctccggtcg ccaggccggt gcagtcgtcc cgtttgatgt acaggttggg
     9361 gccgtcgaga tgacgggtca ggttgggcat gaattcgagc ggcgtcggga aatggccgag
     9421 acgcacacgg gggaaggcgg cgagatgcat gagaaatact cctgattggg ttccatcatg
     9481 gtactcaatg cgcgggtggc tgcccaatgc tatatttttc cgacgccata ccgattttgc
     9541 atagcctgat cacgcacggc aacgaccatc atgaaactac tctggataga agatttcctg
     9601 gccctggcgg acacgggctc gttctcgggc gcggccgcga cgcgctatat cacccaaccc
     9661 gctttctccc ggcgcatcca agcgctggag gcgtggctgg gtgtggagct cgtcgatcgc
     9721 cgcagccagc cgccgcgcct gacggacacg gcgcgccggc atatccccgc gttccgtgcg
     9781 ctgctgcgcg acatgaacca gctgcgcagc cacatgcaat ctgaggcggg tggcgcggcc
     9841 cgactgaccc tggtcacaca acactccctg accatgacgc ggttgccggc cctgctgggc
     9901 cggttttccc ggagccgggc gccgcgcgtg gaattcaccg tgcgctcgga aaaccgcgac
     9961 gaatgcatga ccctgttcat gcgtggcgat gcggatctgc tgctgtgcat ggaagaaccc
    10021 gatgatccgc tatcccgctt gattcccggc gacaggcggc tgtccatggg cgaggaagtg
    10081 ctggttccac tcagcgcacc gggccgcaac gggcacccct tgcacgcgcc acatgccgga
    10141 aagccattga aactgttggc tttccccccg aacagctatc tgggccgggt aatgtacaag
    10201 cgatgtttcg gcgccttgtt ccagacctgc caggtgcaga tcacccatga atccgtgttt
    10261 ctggcgggag tgaaggaagt cctgttggcg ggcctgggca tggcgtggct gccgcgcggc
    10321 ctggtggaac gcgaactggc ttcgggcagc ctgctgatgc tggacgagtc actgccccgc
    10381 gtgaccctgc ggttggcgct gtaccgcagc gaccactgcg cgtatccgga caccctggcg
    10441 cacgcgtggg cactgctgag ccgatgatga atggctgaat gacaccaagc acgccgaccc
    10501 ggcacgggga tgcctaccgg cagtgcggcc cccaggcgat ttcattgaac gcctgttgcg
    10561 gcgtggcccc ctgcttcaac agcgcatcca gactggcctg gtcatcctgc cgctgacggc
    10621 gggccgagtc gtccttgctg ggcgtataag cacgcgcacc cgttatcacc acctggttcg
    10681 ggctgaccac gaaacgctgg aaatcaccgg cgccttggcc ttgtcgaggc gaagccaccg
    10741 agaactcgcc acataaatac cccgatactg aaaacgtctg ctcacgatac cggatgccaa
    10801 cggggttgcc gagcatggcc aacagcgggc catgccattg ttcgatgcgg acatcgtgca
    10861 gcgccttgcg ctgttgcgcg cggaatttcg aatagccgat tcccaggccg acgatgacca
    10921 cgagcaacgc aaccagaaat gaacaaccct tggaattcac gaattgatcc tgtgccgagg
    10981 gggtttgccg ccggcaaacc caacgaaaac gcgaccgccg tattttacgg gtaacacgga
    11041 cacgggtatc gcgggataat gcgggcatgg acgatactgt gcttgccgcg atggcccgct
    11101 ggccgaacgt acccgatgtc ttcggatggc tgtcgctgtc ctccaccgga caatggcggc
    11161 tccatccggg gggcgccgcg ctgccagaag gcatccaagc caacccatca gcgtcgggcc
    11221 ccgacccgga tccggccgcg atgtccgcgt tgtcggaggg cgaagtcatc ggcagccccg
    11281 ccatcaccgc attcatcggt cgcaactacg gccacgacac cgagggtcgc tggttttttc
    11341 agaacggccc gcagcgcgtc ttcgtgcgat tggatgccgc accatacata ctgcggacca
    11401 caggaaccgg cactgcccta cgcacccaca gcggcctggg catcagccac gtcctgtcgt
    11461 gggtgctgga cgattcgggc cgtcttttcg ccatgaccga ccggggcccg ggcatagtgg
    11521 cgggccgtga tctgcccgcg ctggtgaacg cattgcgcac aacgcacggc gagacattgg
    11581 ccgaggcttt ggcgcttggc gttttcgcgg gtgggaacca acccgtcgcg ggtccggtcg
    11641 aacccatcca tatccaaccc atcgacgccc tggccaactc cccgacagag accgccggag
    11701 ccggcgccgc caaggcgcaa agccccgtcc atacggcacc gctgcgattc agcacgacgg
    11761 atgggattgc cgctacactg gggttcacgc gtttccccag agaacgactc gggcctgccc
    11821 gaacgccttt gaaccgtccc caagaacccc gcccatgacc tcgcacgccc cctcgttcta
    11881 cttccccgcc ccccgaacgc agaagttctg tacacaatgc ggcaatgcga ccacacatcg
    11941 cgtcccgccc gacgacaccc gcgaacgcga tgtctgcgaa cactgtgggg ccgtgcatta
    12001 ccacaacccg ctcaatgtcg tgggtgtcgt gccaatctgg ggggaacagg tactgctgtg
    12061 ccgccgcgcc atcgaacctc ggcacggcat gtggacgcta ccggccggct tcatggagct
    12121 cggtgaaacc acggcgcagg gggcggtgcg cgaggccgag gaagaatcgg gcgcgcaact
    12181 cgaactcggc cctctctaca cggtcatcga tgtgccacat gtggagcagg tgcacttctt
    12241 ctatctggcc cgagtgctct caccagacct ggatcccggc ttcgagacac tcgaggcccg
    12301 gttcttcgac acccgggaca ttccctggga cgaactggca ttccgcacgg tgcgcacgac
    12361 cctggaacat tatctggaag atcgtccggg cggacggttt cctgtccatc actattccct
    12421 gatgacccct tcccgcggat gacccgccat gctggtctgg ctcgacgacg cctcgccgtt
    12481 cccggatccg gcaacggccc tgcccgacgg tctggtggcg gtgggcggcg acctgtccct
    12541 cgcgcggttg cgtgacgcct atacgcgcgg gatattcccc tggtacaacg aaggcgaccc
    12601 gatcctgtgg tggagccccg atccgcgcat ggtgctggcc tgtgcggatt tccaggcgtc
    12661 gcattcgctg cgtaaactga tgcgccaggt ggcgcgcacg gaaaccgccc cggacgccac
    12721 cgtacagatc aagaccgaca ccgcgttcga acaggtgatg cgggcgtgct cgcaaccccg
    12781 cgccatgcag acaggcacat ggatctcgcc ggccatccgc gccgcctacc tggcctggca
    12841 cgaagcgggc caggcgcaca gcatcgaaac ctggatcgac ggacggctgg agggcgggct
    12901 gtacggggtg gcgctgggac gctttttctt tggggaatcg atgttccacc agacccgtga
    12961 cgcgtccaaa ctggcgctcg cgtatctcgt ggcgttcctg atgcggcggg gcatcaccca
    13021 catcgactgc cagcagcaga caggccacct tgccagcctc ggcgcgcgtc ccatggcgcg
    13081 cgaccgattc cgggcgctgt tggacgcctc gacctctctt ccggcacccg cgtggccggc
    13141 gggccgcctg gcgcaatccg ggctgcttgc atccgacacc ctacccccgt cataatgaaa
    13201 gcgcccgtcc gggccatgcc gtttcggcga acgccgcccc agacaatgga caatgccgcc
    13261 aagccatgag ccatccgtcc gaatccatct tcaagaccat ccagctgtat tccacggcgg
    13321 cctatccctg cagctacctg tcacggcgcg aagcacgatc gcaagtcgcc gccccctccc
    13381 acatgatcgg ctccgaggtc tattccgctc tggtggaaca aggctttcgg cgcagcggcc
    13441 tgttcacgta ccgcccccat tgcgacacgt gccgtgcctg ccaacccata cgggtcgacg
    13501 tggaccgctt ccagccaagc cgcacccagc gcaagacggg acagcatcac cgcagcctgg
    13561 cggcgcaggc atccgacctg cactgggatc ctgagcattt cgatctgtac aaccgctacc
    13621 agaaacgccg ccatcctggc gcgggcatgg acgaagacag ccaagcccag tacacgcagt
    13681 tccttctgac cagccgggtc gattcccgtc tggtcgaatt ccgcacaccc gaaggcagcc
    13741 tggtcatggt ctccatcatc gacatcctga aaacgggcct gtcggcggta tacacctttt
    13801 tcgaccccga cctgcctggc agcctgggca cctacggcat actttggcag atcgggcagt
    13861 gccgccgtct gggcctgcca tggctgtacc tggggtactg gatcgaagaa agccgtaaaa
    13921 tgcgctacaa atcgcagttc aggccgttcc agatcctgca agacggggcc tggatcgaac
    13981 ccacccccta gtgtgcctgt ttggttaaat cgccacgacc gcaccacaac attcgtccat
    14041 gcccgtcctg ttcaacgcct actcgctggc ccgccccgcc ctgttctccc tggatgccga
    14101 aaccgcgcac gagctaacgc tctcgtcgct cgccaaggcc tatgactgcc cgctgacgcg
    14161 gcggcttctg cacaccagcc cggaactggc gcgcccgctc atgggcatga agctgcgcaa
    14221 tcccgtgggc ctggcggcgg gcctggacaa gaatggcgct ttcatcgaca gtctgggcaa
    14281 cctgggcttc gggttcatcg aagtgggcac ggtcacgccc agagcgcaac ccggcaaccc
    14341 caagccccgc atgttccgtc ttccccgggc acaggctctg atcaaccggc tggggttcaa
    14401 caacaggggg ctggccgagt tcatagagaa cgtcaagaag agccgctggc gccagaacgg
    14461 cggcatactg ggcctgaaca tcgggaagaa tgccgacacg cccatggatc gcgccgtcga
    14521 cgattacctg cacggcctgg aaggggtgta cgcgcacgcc gactacgtga cgatcaacat
    14581 ttcgtcgccc aacacgcaga acctgcgctc actgcatggc cgcgacgaac tcactgcgct
    14641 gctgggcgcc ctgcacgacc gccgccagat gctcgccgac cgtcatggcc gccatgttcc
    14701 cctggcggtc aagatcgcgc ccgatctcac ggttgaacag atcgacgcca tcgccgacac
    14761 cctgccccgc tatgccatcg atggcgtgat cgcgaccaac accacattgt cgcgcgatgc
    14821 cgtgcgcggc ctggcgcacg ctggagaaac aggcgggcta tccggtccgc cggtgcacca
    14881 gatgtcgctg gcggtgatcc gccacttgcg gcaacgcctg ggcgattcga tcgccatcat
    14941 cggggtgggc ggcatcatgt cgggccagga tgcccgggag aaaatcgacg ccggggccga
    15001 cgcggtgcaa atctacaccg gcctcatcta ccgtggcccc gcgctgattc cggaatgcgt
    15061 gcgcgcactg ggagcgtgac acccaagcct ggcttttggc cgggaagcgc cattcaatgc
    15121 gtcccgggct ccgacaagcc tcgtcaacgc cccgccgcgt aagcctggac caggcgcggc
    15181 gacgctgcgg catgcaacag gccatcgggg tcgcgggagg cggccgtgag gcggccgatg
    15241 ctccattccg gcgattcctc gacgacatgg ccgcggcgct tcagatcgtt cagcgcatcg
    15301 gcaccgaaat cgcgctcgat ggtaagactg gccgggcgcc gatcgcgcgg atagaaggaa
    15361 ctggggaaat gctgcgtata gaacatcggc gcatcgatgg cttcttgcag attcatccct
    15421 tgatggacgt gacgcaggaa caattgcagc tgccactgct cttgctgatc accccccggt
    15481 gtaccaaacg ccatccaggg ctcgccgttc cgctgcaaca acgtcgggct caatgtcgtg
    15541 cgcggccgct tgcgcggctg cagcgtgcag ggcaaccctt cttccagcca gaacatctgc
    15601 gcgcgagtgt tcaatccgaa acccagcccg ggaacgacgg gtgacgattg cagccagcca
    15661 ccggatggcg tggcggaaat catgttgccc catcggtcga tcacatcgac atgggtggtg
    15721 tcgcctttgc gcgcggaaac catgcggccg atccgctcca gggccgaggt cgcgaccccg
    15781 tcgggtttga gcgaggacag acggtccagc gcggcgttca cgcgcccggc ttgggcttcg
    15841 tagccgggca cgacgcctgg ccgcaacaag gtcgaagcct gttcgccgat cagcgccgca
    15901 cgagcgcggc tgtagtcatc cgacagcagg tgcgcaagcg gcacatcgac aaaatccgga
    15961 tcgccgtaat aggcttcgcg atcggcgaat gcgagcttca tggcttccat cagcagatgc
    16021 acgaaacgcg ggtcgccgcg cttcagctcg gcgatgtcgg tgtgccgaag caacgccagt
    16081 gtctgcagaa aggcgggtcc ctggctccac ggccccgcct tggagaccgt gatgtcaccg
    16141 taacggtatc gcacgggcgc ctcatagctg gccgaccacg cggccatgtc cgcgccggac
    16201 aacaccccgc gatgcggcga gccgctttca tccatggcct cggtgccgcg cacgaacttg
    16261 tcgatggctt cggccacgaa tccgcgatag aaaacgtccc gggccttctc tatctgccgc
    16321 tcacggtcgc cgcccactga ttcggcctcc gccagcaggc gtatccaggt gcgggcgagc
    16381 gccgggttgc gaaacaacgt ccccggttgc ggcgcgctgc cgttcggcag atacacggcg
    16441 gctgaacttg gccaataggt ctcgaagaat tcctgcaaga ccgcgatggt gtccgacact
    16501 cgctccagca ccggatggcc atgctcggca taatgaatgg ccggttcgag cacgtcgcgc
    16561 agcgagcggg tgccatggtc gcgcagcaga atcatccagg catcgaacgc gccgggcacc
    16621 acggtggaca gcaggccatt gcccggaatc agcgacagcc cttgcgcacg gtaatgatcg
    16681 agggtcgcgt ccgccggcgt tgtaccctgg ccacacagca cctcgatctt gccggtgctg
    16741 acggagtgga agatggcggg cgcctcgccc gccggcccca ccagattggg ttccaccacc
    16801 tgcagagtga acgccgccgc cacgcaggca tcgaacgcat tgccaccacg ctccagcagc
    16861 gacatgccga cggcggtcgc gagccagtga gtcgaggtgg tgacgccgaa cgtaccggtg
    16921 atctccggcc gagtggtgaa catgccgatc ttccttgtca aacggactgg atggtgaaaa
    16981 gaaccataaa gcgggggagt ttattgcagc gtgacaccct tgaggcgtat cagcccatcg
    17041 ggatacggca cgaaaccatg cacccgcttc tgcacgccga aagtccacgg caaatagaac
    17101 aggtagaaga tggggtgttc tttctgcatg ccttgcagga atttcgcata caacattttg
    17161 cgctttcctt catcggtttc ctgcctggct tcgttcaaca gcgcgtcgag cctggggtcg
    17221 cagaacttgc catcgttcag gctgcctttg caactgacgt actgatacac gttgccgctg
    17281 gggtccacgc ggcccgacca acctgcctgt ccaagctgga agtctcccga agccagcgtg
    17341 gattgcagtg ccgcgaactc catgggccgc agcgtgacgt cgaagcccgc ctcgcgctcg
    17401 gtggcctgga tgagttccat cacctgctgc atgatggtgt tgttgccgta cgaaatctgc
    17461 accgccacgc gtttcagacc cgccgccttg agcagcgcct tcgccttggg gatgtcggcc
    17521 cctttgcgcc cgatattctt gtcgtacgcg aaactggccg gcggaaatgg ctggaacgcc
    17581 ggttggtaca gccccatgcc cacgacctgg ttgatagcgt tgcgatcgat ggccagatcg
    17641 aacgcctggc gcacgcgcgg atccttggcc aacgggttgg attcgccgcg cgggccatgc
    17701 gcgaagttga ccgagatcgc ctggaagccc aggccggtga cgggccggaa gtccagcgtg
    17761 gggtcgctcc tgacggcctg cgcatcactg ggggcgaggc gcccggcgat gttcaggtcg
    17821 cccgcgcgca ggttgttgag gcgcaccgtc gagtcgggaa tgggcttgaa gatgacggtg
    17881 tcaaagtggt agtccttggc gtcccagtag cgcttgaact tcgtcagcac aatgcgatcg
    17941 ttctgcacgc gttccttgaa tttgtacggc cccgaacaga ccgggtgctt gcccggatcc
    18001 ttattgaaac tcttgggcga ctgcatcatg ccggcgcgat cggacaattg cgacagcagt
    18061 gacgcgtcgg gttccgacaa atgcagaatc acggtgtgcg ggtcgggcgc gtccacactg
    18121 gccaccgacg cgagctcgct cttgcggttg ctgccgggca gcgtcctcgc acggtccaga
    18181 ttggccttga ccgaggccgc gtcgatgggc gtgccatcct ggtagagcgc cttgtcccgc
    18241 agcgtgaatt tcagcgtctt gttgtccagc caggaccacg acgtggcaag ctgtggcacg
    18301 aacttcaggt ctggcgtgat gtcgaccagc ttgtcgcaca acgaggcaaa gacgatacgc
    18361 cccacgaacg tgcgggcacg caccgggtcg agctgatccg ggtcatcctg cagaccgatc
    18421 ttcaacgtgc tctgggcgca cgccgcaccc gaaatcagaa tggccgcgat cgcggtcaac
    18481 gtcatgcccc tcttgttcat gattgctcct cctcgatagc cccgaatggg acggatgtgt
    18541 cagtaagccc ccactgaatg acgcgcgacg aaatgccctt cccccacttg caccaagggg
    18601 tcgacgcgtg gctcgtcgcc caccttgcgc accgggctgg ggatctcgtc caccagcaac
    18661 gaatgctctc ggtggcgccg cgatgggtcg gcgatgggca cggcggccat cagctttctt
    18721 gtatagggat gctgcgggtt ctcgaacacg gcgcggcgcg ggcctatctc gaccacctgc
    18781 cccagataca tcacgacgac gcgatggctg atgcgctcca ccacggccat gtcatgcgaa
    18841 atgaacagaa tggacaggcc gagctcgcgt tgcaggtcca gcagcagatt gacgatctgc
    18901 gcttgaatgg acacgtccag ggccgacacg gactcgtcgg cgatgatcac acgcgggtcc
    18961 agcgccaacg cccgcgcgat gcagatgcgc tgacgctgcc caccggaaaa ctcgtggggg
    19021 tagcgatcgg ccatttccgg cgacaagccg catttttcca tgagccaggc gacccgttcc
    19081 tgcgccttgc ggccgctggc cacgcgatgg atcagcagcg gctccatgat ggaatagcca
    19141 acagtcgcgc gtgggtcgag cgaggcgaaa ggatcctgga aaatgaactg gatattccgc
    19201 cgcagagcct gcatgcggct gccgctcagt gcgccgatgt cctgcccttc gtagaggatg
    19261 cgaccggccc ggggcgttcc cagcagggcc agtgagcgtc cggtggtggt cttgccgcag
    19321 ccagactcac cgaccagagc cagcgtttcg ccgccgaaca ggtcgaacga cacctgctcc
    19381 accgcatgca cgcgtcgccg cacttggcca aagaagccat cggcgatgtc gaagcgtgtg
    19441 gtgagaccct gcacccgcag cacgggcgcc tcgccgtaac gtgccgtggt ggtcacgggc
    19501 cgcagcccgg gtgtgctggc ctgcccatca cgcgtcgcgc cctcgcgcaa caattcgaat
    19561 ggcgcgggct gatccagccc cttcatggag cccagtcgcg gcaccgccga cagcaacgcg
    19621 cgcgtgtacc agtggcgggg cgtgaggaaa atgggttcgg acggaccttc ctccaccttt
    19681 tcgccgtggt acatgaccat gacgcgatca gcgacttccg ccaccacccc catgtcgtgc
    19741 gtgatgaaaa tcacgcccat gtccatgtcg cgctgcagtt cgcggatgag ttccaggatc
    19801 tgcgcctgaa tggtcacgtc gagcgcggtc gttggctcgt cggcgatgag cacctgcggc
    19861 cggcacgaca acgccatcgc gatcatgacg cgttgtcgca tgccgccgga aagctggtgg
    19921 ggatagcggc tgcacaacgc cctggcatcc gggatgcgca ctgtttcgag caggcgcagc
    19981 gtctcggcct gggcggcgcg ggcatccatg ccttgatgca gccgcagcgc ctcggtgatc
    20041 tgccacccca ccgtgaagct cgggttgagc gacgtcatgg gttcctggaa gatcatggcg
    20101 atgtcggcac cccgcaccgt gcgcatgacc gactcttcgg cgcgggccac gtcgatgacg
    20161 tccccattgc gtcggcgcag cagcagccca ccctgtgtga tgcggccccg gccgaactcg
    20221 accagccgca tcaacgccag cgacgtgacc gatttgccag agccggattc accgacgatg
    20281 gccagcgtct cgccacggtt caggtggaac gacacatcgc gcaccgcgtg cacttcccgt
    20341 tccggcgtct tgaaggaaac ggacaggttc tcgacctgga tgacgcgttc ggcattcatg
    20401 ctcggcatcg tcagtcctcc tggcgtgggt ccagcgcatc gcgcaagcca tcgcccagca
    20461 gattgaaccc gaacaccacc aggaagatcg cgcagccggg gaacacggac atccacggcg
    20521 cctggatcat gaagttcttc gcggtgttga gcatcgagcc ccacgaagga tcgggcggtt
    20581 gcaagcccag ccccaggaac gacagactgg cctcggcgat gatggccttg gcgatctgga
    20641 tggtggcctg cacgatcagt gctggcagga tgtttggcag gacatgacgc aggatgatgc
    20701 gacgatccga tgcgcccaat gagcgcgtgc tctgaacgta gtcctcattc ttcacggcca
    20761 gcacctggcc gcgcgccagc cggatgaaaa gcggcgcggc cgacaccccg atggcgatca
    20821 tggcgttgat caggctgggc ccaagaaagg ccgccaacgc gatggccaga atcaggaacg
    20881 gaatcgagaa cagcgcttcc gtgacgcgcg agatgcagcc atcgatcagt ccgccgaaat
    20941 aacccgacaa cagaccaagc ggtacaccaa ccagcagggc gatcagcacc gagcacatgc
    21001 ccgccaggag cgaggcgcgc gcgccataca ccatgcggct gaagatgtcg cgccccagct
    21061 catcggtgcc gaaccagtgc gccagggacg gcgccttgcg gatggcggtg aagctgacat
    21121 gcagtggatc ctgtccggcg atcagtggtg ccagcagcgc cagaaccacg aagaacagga
    21181 tgatgatcgc cccgagcatg gcggaacgat tgcggcggaa tttcgcccag gcacggctgc
    21241 ggcggcgcgg cgccgcgccc gtgacgggcg cgttgacggt agaggcagtc atgaatttct
    21301 caagcgtggg ttgacgagga tatacagcac gtcggcgagc aatgtaagaa cgatgaaacc
    21361 cgtggcaatg cacagcacca caccctgcac cacggcgtag tcacgattga acaccgcatc
    21421 gaccagcaac ttgccgaagc cggggatgcc gaatatctgc tcggtgagca ccgcgccggc
    21481 cagcaactcg ccgaacagga tggtgaccag cgtgacgatg ggcatcagcg cgttgcggaa
    21541 cgcgtgccgc agcaccacgg accattcgga cgcgcccttg gcgcgcgccg tgcgtacata
    21601 gtcggcgctg agcgcctcga gcatggccga gcgggtgtgg cgcatcaggt aggcgctgat
    21661 gacggcggcc agcaccaggg agggcatcag ggatgtcttg atggacagcc agaaatcctg
    21721 tgagggcggc acgaaccccg atgccggcaa tagccgccaa tgcaccgaca ccaacatgat
    21781 gagcaggatg cccagccaga aattcggtat ggacattcct gataaggcga acaagttcgc
    21841 gccgaactcc acgcgcgtgc ctttcttgac ggcggcgagt atgcccagcg ggatgcccgt
    21901 caccagcgcg aacagcatgg ccatcaccga gagctgtatc gtcaccggca gcttctgcgc
    21961 caccagcgtg gtgacgggga tgtcggtgcg cagcgacttg cccaggtcgc cgtgggccac
    22021 tctcttgatc catgccagat attgctgcgg cagcggatcg ttcagatggt atttttcacg
    22081 cagataattg aggacggccg gatcgcgctc ctcaccggcc atggccagca ccgggtcgcc
    22141 gggcaggatc ttctgcaggc tgaagatcat catcgagacc aggatcagcg tgggaatcgc
    22201 cacgatcatg cggcgcacta tcagtttgaa catccgtggg ctccggcggg gcttgaaaca
    22261 agggatgccg acctccagga cactagaggc cggcgtccat gcggcgcgag ctagttcttc
    22321 gcgagtgtca cgccgcgcag gcggatcatg ccatcggggt acggcgtgaa cccgtgcacc
    22381 tgcttgcgta tcgcgaacgg ccagggcatg tagtagatgt agagactagg caaatcgtcc
    22441 tgcaggatag cctgggcttt gtcgtagatg gccttgcggc tggccacgtc gggcactgtg
    22501 cgagcctctt tcaggagctt gtcgacctcg gggttgcaat acttgccgtc attcaacgac
    22561 cctttgcagg tgacaaagtt gaagatattg ccgtccggat cgacccggcc cgaccagccg
    22621 cgcaagtcaa tctggaactt gccttgacta tcgtcggcca gcatggccgc gtattcggtc
    22681 gggcgcaggc tgagttggaa acccgcctgg gcggccatgg cctggaccat ttcggcggtc
    22741 gtggacgaga tcgtgttgtt gccgaagctg agttccgcct tgaccaccgg catgccggcc
    22801 ttcttcagca acgccttggc cttggcgatg tcgggtttga cgaccgggaa cttgtcgcta
    22861 tggtaggggc tggccagcgg gaacggctgc tgggccggtg tgaagatgcc gttgccgatg
    22921 acctgattga tggcgtcccg gtcgatggtc aactgaaacg cctggcgcac cagtttgttg
    22981 gcgaacggat tcgtcttggc cctggggcca ttgccaacgt tgaacatgaa ctgctggaag
    23041 cccaggcccg tcaccggctt gaactccagg ctggggtcgc tcttgactgt cttgatgtcg
    23101 gacgggttca ggcggcccag gatgtcgatg ccgcccgagc gcaggttctg caggcgcacg
    23161 gtggtatcgg gaatgggcag gaacaccacc ttgtcgaagt gataatcctt ggcatcgtag
    23221 tatttctcga atttcttgag cacgatgcgg tcattctgca ctcggctgac gaactgatag
    23281 gggccggagc acaccggatg gcgagcgaca ttggccagat ctcctgacgc gaaactcttg
    23341 ggcgagagca tcatgccggc gcgatccgac aaatgcgcca gcaacgtggc atccggctgc
    23401 ttgaccttga tgatcaatgt ggtcggatcg ggcgcctcga tgtcctgaat ggaagccagt
    23461 tcggagcggc gctggctgcc cttcagcgtc atgtcgcgtt ccagattggc cttggcggcc
    23521 gcggcatcga aacgggtgcc gtcctggaac aacgcgtcgt cgcgcagctt cattgtgagg
    23581 gttttattgt cgggactcca ggaccacgac ttcgccagct tcggcacgaa ctgcagtttt
    23641 gcgttgatat cgaccagctt gtcgcacaac gacgcgaaca cgacgcgcga cacataggtg
    23701 cgcgcgcgcg ccggatcgag cgtgtcggga tcttcctgca aaccaatgcg caacgtctgc
    23761 gccacgctgc aggccgtcgc ggcccccaac aatatgccac ccgccaccag aacgatcttc
    23821 ttcatcactc agtctcctat atattgtttt tgcgacaacc cgacgccgcc tcgtcaaaaa
    23881 ccgacggcct ggccatcgcg ccggctgtcg ctcgccgcca cgtagccatg attcgggtca
    23941 tccgacaatt tccagacgaa ctgtccggcg ccgaagtcca tgtaaggatc gttgacggat
    24001 ttcagcacgt gcccaaggtc cttcaacccg ctcaccgtgt cggggttcat gttcggttcg
    24061 atatccagac tgaagtcggt gttgaccttc cagcgcggtg cacagcacgc cgcctggggc
    24121 tgctggtgat aatcgaccat gcgcacgatg gtctgcaggt gcccctgcgg ctgcatgtca
    24181 ccccccatga cgccgaagct catgaccggt ttacccccac gggtgaggaa tccgggaatg
    24241 atggtgtgga atggccgctt accgccctcg accacattgg gcgaacgtgg atccagagag
    24301 aaccccatgc cgcggttctg catgctgatg ccggtgtccg gcaccaccac gcccgagccg
    24361 aatcccatgt aattcgactg gatgaaggaa atcatcatgc cgctctcgtc ggcggccgac
    24421 agatagatcg tgccaccggc gtgcggccgc ccggcttgga aagacccggc gcgatcgagt
    24481 ttgatcagct tcgcgcgacc gcgcagatag tcgtcgtcga gcaattgggc cgaggccacg
    24541 tccatgctgc gcggatccga cacataacgg tagagatctg cgaacgcgag cttcatagcc
    24601 tcgatctgca cgtgctgcga ggtcacggaa tcgaccggca tgctctgcag gtcgaactgc
    24661 tcgacgatgc ccagggccat gagcgcggca atgccctgcc cgttgggcgg gatttcgtgc
    24721 acggtatagt cgcgatagtt cttggcaatg ggctccaccc attccggacg gtaattgcgc
    24781 agatcatcga gcgtcaggga tccgccgcct tcgcggctga aggccacgat cttctcggcg
    24841 agctcgcctt tgtaaatatc gcgcccccgg gtttcggcga tgcgccgcaa ggtgcgggcc
    24901 acgcccggaa ggcggaacaa ctcgcccacg tggggtgccc tgccgtgcgg caggaacgcc
    24961 tgggcatagc cgggttggtt gcgcaattcg tccacggcct tggcccattt cccggccacg
    25021 acggggggca cggcatagcc gcgttcggcg acctcgatgg cgggctcgaa caggtcctcg
    25081 aaaggcagct tgccgaattt ttcgtgcagc gccgcccagc ccgccaccac accggggacc
    25141 gtgacggcgt cccaaccacg cgtgggtcgc gtggccaggc cattgccgtc gaccccgtac
    25201 ttgcgcttga aataatcaac gttccacgcc gctggtgcga cgccagagga attcaagccc
    25261 tgcagccgct tgccgtccca caccagtgcg aagcagtcgc ctcccagacc gttggacacc
    25321 ggctccacct gaacaagggt ggccgcggcc gcaagtgcgg catccacggc gttcccaccc
    25381 ttgagcagca tgcgcaatcc cgcctgcgcc gcgagcggat gggatgtgga tacgacgttc
    25441 cgggcgaaca cgggcgtgcg ggttgaagga taagggttgg tccagtcgaa aggtttcatg
    25501 ggaaggcccg ggcggtgcgc cgcgatctgt gtttcaggga actggataag ttactatccc
    25561 cttatcatta aatcaattta gatttaattt tcatacaaca agtttttctt atacatgggc
    25621 tctcttcgtt ttctgcgcac gttcgtcgcg gtcgctcgcc gtggctcgtt ctcggacgcc
    25681 gccgaacagg ttgcactcac ccaggcggcg gtcagtctgc agatgcgcgc cctggagacc
    25741 gaattcggcc gcacgctgtt cgaccgctcc ggtcgcctgg cttcgctgaa cgaggcaggc
    25801 cgcgaattac tgccgcaagc cgagcaattg ctggaaatct acgaccggat gcgcacaccc
    25861 cgccccgctc cgggtacgtt tgccggcaca ttgagcatcg gcgccatcgt ctcgtgcatg
    25921 ggcacgctgg cgcgcgtggt ttctcaactc aagatcgagc accaggaact gatcatccgc
    25981 ctgtcctcgg gcaagtcgcg tgagctgtgt cgcaaggtgg agaacggcga gatcgacgcc
    26041 gccttggtgg tcggcgccgg acggcctcca cctcgcacac gctggaaacg gctgtaccag
    26101 gaaccagtgt gtctggtcgt gtccagccag gtgcccggcg atgaccccag gcaactgctg
    26161 cgcagccacc cattcctgcg tttcgaccgc agccagcaca cgggcgcgca gatcgaccgc
    26221 gtgctacgga aaatgggcat cgtgcccgat gactttctgg agctgaacgc gttggaacaa
    26281 ctgctggatc tggtccggca cgatgtgggc atcaccattc tgccgttgtt gcacactatg
    26341 cgctggaacg acggcaatgg gttgcgcgcc attccgctcc ccgcggaact gggcgccatc
    26401 acccgtgaga tcggcatgct cgaacggcgc gaccacaatc tccacgcggc cgccgccatg
    26461 atttttgagc gctacgcggc gctgtcggcg aaaggctgat gaatgggttt cccccgccgc
    26521 gccggaacgt tctgatcctg gggtctacac gttccgaggc tccatcagcg caaggatcgg
    26581 gcagggaagg cagcgatgga ggctcaccac tgccttccct gcccgatccg actaaagaac
    26641 caaagagtcc gcctttgcaa agcctcgaaa agtaggcacc gggaccgaaa ctcttcagat
    26701 gaaacgaagc ccccggggaa acttccccgg gggcttcttg acccggaacc tctacggtga
    26761 ttccggatac ttccctaacg aatcgccgtg atcaggcggc ggcgcggcgg gcgcggggag
    26821 ccggcttggc ggcctcggcg acgtcggacg cggccttcag ggtggcgttg gtcgcggcgg
    26881 cgatgttcga ttcagccgtc ttggacgcct gcttggcagc cttggcaacc gactcgtagg
    26941 cgctggtggc cgtcgacagc gacgacttca gcaacgcaac ggcgctttcg gagccggcgg
    27001 gcgcgttctt ggaaagctgc tcgatggcct cggagacctg ctgttggttc tgggagatct
    27061 gctcttcgcc cagcttggtc aggtcggctt gcacaccgct gacgatgtcg tacacgtgct
    27121 tgccgtaggc cagagccttt tcggcgttgg gctgcgtcag accagacgca aacgcgatgg
    27181 cttcctgcgc atccttgagc ccgatgactt catgcgtctt ctgggcggct tcttccaggc
    27241 tggccttgac gaccttcagg ttcagatcga ccagcttttc gaaaccttcg aaaaacgaat
    27301 tctggatggc aaccaggttg tccagcgagg ctttctggct ggcgaggatg tgttggggga
    27361 tagcgctcat aatatgactt ccttcatgcg gccctggcta agccgggcct ggacgatcga
    27421 accgagttca aaaaacgtgg caagaaggtt tcgtgttcac tgtgttctgt gacaaccttc
    27481 gatgttgcat tgcacaatcg tcattctaca gattcggttg atcgcgtcaa ggaatattta
    27541 ttgcagcgca gcaaattaat tgaatcaaca tgttacgaca ctctgtccga cacgggccgc
    27601 ccctcgtagc ccagggcgcc ggatatgtgc gaggccgtct tgaccagtgc ctggatccat
    27661 tcatcgcgca tgcgttcggc cggcgccgac aatgacagcc cggccagcaa ttttccggtg
    27721 tcatcgtaga cgcccgcggc gatgcaacgc acacccagtt cgagctcttc gttgtcgcgc
    27781 gaatacccat ggcgccgcac cagggccagg tcacgctcga gatcatcgag cttggtgatg
    27841 ctgttgcgcg tggtgcccgc cagccctgtg cgcatggcat aggcccgcac ctggcgcgga
    27901 tcgctgatgg acagaaacaa cttaccagtg gaggtgagat gcagcggcgc ccgaccgcca
    27961 atggcgcgca ccacctgcat gccggagcgt tcgctccagg cgcgttctat gtagacgatc
    28021 tcgtccccct ggcgcagcga caggttgacc gtctggcctg tgagacgatg cagttcgcgc
    28081 atgggctcca tcgcgacctc gcgcacatcc agccgcccct tcaccaggga acccaactcg
    28141 agcaggcgca tgcccagccg atacaacccg ctgtcgacgc gctcgacata acggcccacc
    28201 accaagtcgt tcagaatgcg atgggtggtg gacgtgtgca gatgcgtggc ggcggccaag
    28261 tctttcagcg tgacaggctc gacatggtcg gccaggacat cgagcaacag catggccctt
    28321 tccaggacct ggaccgtgac ggcagaactg ttgaccggcg aaagcggccc ggaagagtac
    28381 gacagggatg gggttcggga catagcctgg aattgacgcg acgcagcacc aaaattccat
    28441 actatgaaaa aaatcgacag atcgcaagcc ccatatcacc tctcatcaca aaaaacaccc
    28501 agaaaccgcg taagagcctt gtgcgctgaa actgggaagc cgaatactca acttcggagc
    28561 ccacgttccg cacgacttga tccgaggagc cctgtttcct tatgtgttta tgtgcggcat
    28621 tgtcggcggg gtttacttct cgggcggggt gagcgacccg cccacccgaa cgacttcgtc
    28681 gcgtagatac gccaacgcct gggccgcggc atcgggctca cccttgacgc ccaggtcgat
    28741 gtgcggatgc cccgacccgc ccatgctggg cagactgaac gccttgacgc ccggccattt
    28801 ccgttccagc tcctcgagcg ccggcgtgat gcgggattcg ggcaaccgaa aggccaggaa
    28861 cgaatgctca acccgcgtca ccttgtgctg cgagccggca tagcgggtgt cgagcgtcca
    28921 ttccatcatg ggccacgcca tttcggggaa gccgggcatg aacgtatggt tgtggatgaa
    28981 gaaaccggga atcttgttga acggattggg aacgatctcg gctcccttgg gaaacacccc
    29041 catctgcagg cgctgctggt tttcgggcaa cgacatgtcg gcactgccac tgccccgccg
    29101 cgcgttgtcg gcgcagcgtt cggcgatgag ccgggccgct tcgggatgca gttcaagctc
    29161 gagtcccagt gcctgggccg ccgcctgccg agtctggtca tccggcgtgg cgccgatccc
    29221 accgcagcaa aacacgacac cgccactcgc gaaagtgcgt gtgagcacag ccgcgatggt
    29281 gtcgcgatcg tctggcacga actcggcgcc ggccagttgc atgccgcgct gcgaaagtag
    29341 ctcgatggcc ttagagaaat gcttgtcctg acgtcggcca gacaggattt catcaccgat
    29401 gatgagcagt ttcacttgcg aagcgacagt cgacatgggg caaccttgtt caatgaacga
    29461 ttgaaaacgg aagggaacgg tggagtggtc ggcgggagtc aagcctcgag caccgtttcc
    29521 cgtctgagct gacgcagggc ttcaaggctg aaatgcgcga acaccagcgc ggagaaaacc
    29581 ggcaacacca accaggccgg cggaaacagg ttgaggacag cgaggcagga gccgatgagc
    29641 cagagctgcc ggttgtggcg gcgccagaca atcctgcgtt cccgcgcact ggcgtgctca
    29701 acaatagcgt cgacgcgcag catccgagtg aacgcgaacg cccaccagaa caccggcagc
    29761 acgacggcga ggggggggat caaccacagc ggcatggtca gcgcccagcc gatgatgaac
    29821 agaccaccca cccaggcggc attccacgcg ctgaccgtgc ccgcgaactc gccctgccgc
    29881 ctcaatccct tgtattcacg gtgctccaga tggttgagca cgatgggcat cacgaaaatc
    29941 gcggcgatga ccagccccag aatccccgac agcggcagca ggatgcacag ggccagaaag
    30001 gggatcaggt acagtttcag cgaaaacagg cccagggcca gcaaccattg atccacatcg
    30061 ttgagatagg tccagctcgc ggattccccg cctagccagt gcgtgagcgg tgtccagaaa
    30121 atccacaaca gcaggatggc gcccagcaac gcgatgatga acggcagaaa cagcgccagg
    30181 atcattctgg gatgacactg cgacaccagc gcgcgccgga aggcgaggcc cacggcagcc
    30241 aggcccgtgg cgggcggggc gcccgcgggg cgggccacca gatcaaagtt gctcatggtt
    30301 gcgacagact cagacggaaa acacgattat catagttaaa acacaggaag tccgcgcgca
    30361 tgaccgtctt caatccgtta cccgatcttt ccgccctgcg ggcacggctg gttgaaagcc
    30421 atgagttgat cgtggcctgc tattgcgcgc aatggtgtga cacctgccgc gcgtatcggg
    30481 ctgatttcga aaacctggcc gcgcaatggc cccggcatgt gttcgtatgg atagacatcg
    30541 aggacagccc cgatctgctg ggggacgtgg acgtcgagaa cttccccacg atcgcgctgc
    30601 aggacgcgcg gcgcaccgtg tttttcggca cgctgctacc ctacgccagc catctcgatc
    30661 ggctcattgg gcgcagcgac agcctgactg atacgcccac cgaggccccg cccctgctgg
    30721 gtccgttgct cgtgacagaa acagccggaa aataaacagc cagctagcgg ccccggccgc
    30781 ccagcaacac ccccaccgac ctcttgtgtg cgtgcgtgtg atgcgaagtg ggtgcgttca
    30841 acaccgcgct tgcaggcgcg ggcgacggca catagggctt gtggaagaaa tcatccaccg
    30901 ggcgtgttgg ccgaccaccc gatctgccgc gcggagcacc gccctgccct tgaggggggc
    30961 gatacgcctc gatggggagt tcgagttgcc cgcgcggaat cttcgacttg atgagcttct
    31021 cgatgtcgat caggaaccgt tcttcgtcgg gggcgaaaaa ggcgatggcc tcgcccgatg
    31081 cgcccgcgcg cccggtgcgc ccgatgcggt ggacgtagtc ttcggcgttg aaaggcagat
    31141 cgtaattgat gacgcacggc acaccagcca catcaagccc gcgcgccgcc acatcggtgg
    31201 cgaccagcac gtcgagctgt cccgccttga aggcgtcgag cgccttcatg cgatcgagct
    31261 ggctcttgtc gccatggatg gattccgcct tgacgccatc gcgttcaagc tgacgcgcca
    31321 agcggctggt gccgatcttc gtgttcgaaa acacaatgac ctgactcagc ccgcgcgact
    31381 tgaccagatg caccaccgcc gcgcgcttgt cggcgctcga aaccgggtag gcgatttgcg
    31441 agaccgtttc agccgtcgcg ttgcgggcgg ccacttcgat ttccaccggg tcgtgcagga
    31501 aactgcgcgc cagcttgcgg atttcattgc tgaacgtggc ggagaacagc aaaccctgac
    31561 gtttggccgg cagcagacgc atgatgcgat cgagatcggg caggaagccc atatcgagca
    31621 tgcggtcggc ttcgtcgaga accaggatct cgacctgcgc caggctgacg tttttctgtt
    31681 caacgtggtc cagcaagcgt ccaggcgtgg cgatcaggat ctcgcaaccg cgccgcaggg
    31741 cgtcgcgctg cggacggata tcgacaccgc cgaacaccac cgtggaacgc aggggcgtgg
    31801 ccttgctgta gttggccacg ttctggtaga cctggtcagc cagttcccgc gtgggcgtga
    31861 ggatcagcgc gcgcactgga tgccttgccg gcgacgcgct gtgcgtggcc aagggcatga
    31921 gccgatgcag gatgggcagg gtgaatgccg cggtcttccc ggtgcctgtc tgggcggccc
    31981 ccatgacgtc gcagccatcc atgacccggg ggatggcctg tgcctgaatg ggggtggggg
    32041 taacgtaccc ggccgcggtg accgcctgaa gaatgccagg atgcaagccg aaatcggaaa
    32101 acgtggtggt tgtgtcggtg tgggtggagt ctgtcattga gtggtgtggt gccgcacaaa
    32161 aggggtgtgc caggcgttat aacccgcaca gtttactcca cgcgccatcc cgattcgacc
    32221 ggacagtctg gacacctcca aaaagtgtcg ttaaaaatca acggattaac caggaaacgg
    32281 caacgcccgg gccccaccca aaggcccgaa cccccgcgcc cacgggcctc ggcgcacttg
    32341 gtacaatgtt acgttacctc tcgggagagc aatatgtcga tgtcggatcg tgacggcttc
    32401 atctggtatg acggcaaact ggtgccctgg cgcgacgcca cgacccacgt cctgacgcat
    32461 tccctgcatt acggcctggc cgttttcgag ggtgtgcgcg cctacaagac caaccagggc
    32521 acggccatat tccggctgca cgaccacacg aagcgtctgt tcaattcggc ccacatctac
    32581 cagatgccca ttccgtacga tcgcgacacc atcatgcggg cgcaatgcga agtcgtgcgc
    32641 ggcaaccaac tggagtcgtg ctacatccgc ccgctggttt tttatggttc ggaaaaaatg
    32701 ggggtttcgc ccaagggcgc gtcggtgcac gtcgccatcg ccgcctggcc ttggggcgcc
    32761 tacctgggcg aagaggccct caagcaaggc atccgcgtca aagtgtcgtc gtacgcgcgc
    32821 cagcatgtga atgtcaccat gccccgggcc aaggtcgcca gcacctacgc caactccata
    32881 ttggccaatg ccgaggctct ggaccacggc tacgacgaag ccattcttct ggacaccgaa
    32941 gggttcctgg ccgaggggtc gggcgagaac attttcctcg tcaaggacgg cgtcctgtgc
    33001 gaaccggaaa tcgcgtcggc cctcaccggt atcacgcgct cgaccatcca tgcgttggcc
    33061 gccgacctgg gcatccccgt gcagaccaag cgcctgacgc gcgacgacct gtacatcgcc
    33121 gacgaagcct tcttcaccgg caccgccgcc gaagtcaccc ctatccgcga agtcgacgga
    33181 cgcgtcatcg gaccgggcca acgcggaccc atcaccgaaa aactgcagca agccttcttc
    33241 gatctcgtca gcggcaagaa cgccaagtac cagcaatggc tgaccccggt ttaaggcctg
    33301 ttcccacaga acgagctcgc ctccagacac ttccccctac ccaggacacc ccatgagcag
    33361 cacaaccgcc acccgccacg acatcatcta cgtcgaggcc aaagacctgc cagtctattg
    33421 cccgggcccc aaggctcctc tctggagcat gcatccgcgc gtctacatcg aagtggccaa
    33481 gaccggcacg gcggtctgcc cgtactgcag cgccacctac cagttgaagg aaggcgagaa
    33541 aat
//
LOCUS       DASTNK010000070         7716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_605384, whole genome shotgun sequence.
ACCESSION   DASTNK010000070 DASTNK010000000
VERSION     DASTNK010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7716
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605384"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1081)
                     /locus_tag="VFR20_05505"
     CDS             complement(173..1081)
                     /locus_tag="VFR20_05505"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEU0230008.1"
                     /translation="MILLIDSGNTLVKFGWLDPATGQRETTPLSLAHDDTANLADWLD
                     RLPAQPARAVGVNVAGRERAQALETHLGRRGCAVRWVTSLDHARGVRNAYDPPGQLGP
                     DRWVSLIGLSRRLRNAATSVPAPIVMLATFGTATTIDTLVPDTIDGARPNQWAGGGES
                     GHVERASNPREWVFRGGLILPGPAMMRSALAHGTALLPDADGSLAGYPTHTRQAIATG
                     IAAAQAGALVRQWAVGLEQYGQPPRVYCAGGGWPAIEPEARHQIARAQAQAGVEPRPI
                     DWLPTPVLDGLATMALADVPAPQYSV"
     gene            complement(1087..1950)
                     /locus_tag="VFR20_05510"
     CDS             complement(1087..1950)
                     /locus_tag="VFR20_05510"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEU0230009.1"
                     /translation="MASTLPLPLPEPGQFMTALRSALPHFGQVEWFARTASTNADLLT
                     KTRTSLLRLDRPWLLGAHLQEQGRGRAGRTWQNRPGANLMFSCAFDVFLPPRLLPALS
                     PLAGVAACEALRGLLPPPLQPHLTLKWPNDLQWKFAKLGGILVEITRAGTSRLSADHH
                     VAIMGIGLNLDDARSLSQSLDRQIADWREICDAAQEVAQSPATALVSRIAQAWYDGIN
                     RAIRQGLLEFPARFAQVDALNGQHVNILDDGQVVHSGIACGVNELGQLLLRTPAGELA
                     ISVGDVSVRPK"
     gene            2086..2739
                     /locus_tag="VFR20_05515"
     CDS             2086..2739
                     /locus_tag="VFR20_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0230010.1"
                     /translation="MFARIENRTFQGRAGDVDCLIEWPDGPARGWALALHPHPLHGGA
                     RENKVVTTIARACLQHGLATVRPNFRGVGKSAGVFDSGVGETADMHALVEQFSRAWPE
                     EAAGRWVLAGFSFGTSVAAQLYSVLAEQGGATPDVLMLLGSAVERFRFRDVVVPDDTF
                     LVHGELDEVVPLAEALDFARPRSLPVVVVPGASHFFHGKLVVLKQLLAQRLMALGHT"
     gene            2976..4265
                     /locus_tag="VFR20_05520"
     CDS             2976..4265
                     /locus_tag="VFR20_05520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEU0230011.1"
                     /translation="MPTISSPISFLHRVRRGISILTLAVAPAAVLAATPAAPARVQPA
                     APAQPAAPAQAASAIPVPAAVRGLTPVGDLSKVPAPTIDAKAWLVLDANSGQVISAVD
                     PDEKIEPASLTKLMSIYIVFESLANKRLALDQKVNVSEKAWRTGGSRMFIKVGTQVSV
                     DDLLQGVIVQSGNDATVALAQAVAGSESAFVSLMNQEAQKMGLTNTHYVNVDGLPDPA
                     HLTTVRDLATLARNLVTRFPQYMHYFSEKEFTYNKIKQRNRNRLLWADPTVDGLKTGH
                     TESAGYCLVATAKRDGRRLVSVVVGAPSDSARTETSLKLLNWGFQNFDTIKLYDNTHA
                     AVQARVWEGTVETVGLGQPDATWVTVPRGEGQQVKPIAQYTQPLVAPLKQGARVGEVS
                     LTLDGKVLRRDPLVILKDVPQAGFFGRMFDKVRLMFK"
     gene            4424..5287
                     /locus_tag="VFR20_05525"
     CDS             4424..5287
                     /locus_tag="VFR20_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HEU0230012.1"
                     /translation="MIPDVDNDSIVYVNGEHVRLGDAKISVLDRGFIFGDGIYEVVPA
                     YNGKPFRMKEHLARLQRSLGAIRIETGKSTADWENLVHDMLKRSGLGDCMVYLQVTRG
                     VAKRDHGFPQDCEPTVFCMVSPFKRPDAHKREDGLSVVSMPDSRWLHCEIKSVSLLGN
                     VLAKQYAVDAGVDEVVQFRDGHLSEGSSCNIWVVKDGVLLAPVRDHYILEGIRYAFLE
                     QLAAKAGVKFQSRVITKQEVEEADELMLSSATKEVLPITRYEGKPVGQGKPGPVYAKL
                     RAGYDEAIARL"
     gene            5301..5588
                     /locus_tag="VFR20_05530"
     CDS             5301..5588
                     /locus_tag="VFR20_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HEU0230013.1"
                     /translation="MEPAMKAIPPEESLIEYPSDFPIKVMGKSHPDFAQTLTEVVMRF
                     DPAFDAATVEIRPSKAGNYLSLTFTVRATSREQLDNLYRALHEHPMVSVVL"
     gene            5603..6241
                     /gene="lipB"
                     /locus_tag="VFR20_05535"
     CDS             5603..6241
                     /gene="lipB"
                     /locus_tag="VFR20_05535"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEU0230014.1"
                     /translation="MIDVSWLPRPAPYQEVWQAMRDMTEARDDSTHDAIWLVEHSPVY
                     TLGQAGKPEHILHADGIPVVHSDRGGQVTYHGPGQIVAYCLVDLRRLGVYVKEYVVML
                     EDAAIATLAELGVANACLKPGAPGVYVPMPEGDLAKIAALGIKIRNGRGYHGLALNVD
                     MDLSPFSGINPCGYEGLRTVDLATFGVRVPLDQAAEVLSRHLVRLFESHVAH"
     gene            6295..7080
                     /locus_tag="VFR20_05540"
     CDS             6295..7080
                     /locus_tag="VFR20_05540"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU0230015.1"
                     /translation="MSSENAVLLVDQIDGVYTLTLNRPGQFNALSEELLAELQRNLDA
                     IARDDDARCVVLAANGKAFCAGHDLKQMRAHPDHDYYARLFADCGRVMQSLVELPVPV
                     IAKVQGIATAAGCQLVASCDLAVAVDTARFAVSGINVGLFCATPAVALTRNVPMKKAF
                     EMLITGRFISSAEAVDHGLINQAVDAERLDSVVAALVQTICAKSPLAVRTGKGMYRRQ
                     HGMALADAYDYAGRIMARNMMTEDVCEGIDAFTQKRAPVWKGR"
BASE COUNT         1291 a         2397 c         2611 g         1417 t
ORIGIN      
        1 gactgatcaa ccgtcgtata tgccgcccgc tctgctctca gcgcattgcg cttaaatgcc
       61 cgaacgcggc tcgctaatcc attggcttcg cgttcgggga aaagagataa cgtagttgac
      121 aaggtcaata catatcagtg cggtgtttga cgtttttatg ttccggatgg cgtcagacac
      181 tgtactgggg tgcggggaca tcggcaagcg ccatggtggc caatccatcg agaacgggtg
      241 tgggcagcca gtcgatggga cgcggctcga cgcccgcctg ggcttgtgcg cgggcgatct
      301 ggtgacgcgc ttcgggttcg atggcgggcc agcccccccc ggcgcagtag acccggggcg
      361 gttggccgta ttgttccagg ccaaccgccc attgtcggac cagggcgccg gcctgggccg
      421 cggcgatgcc ggtggcgatg gcctggcgcg tgtgcgtggg gtagccggcc aggctgccat
      481 cggcgtcggg cagcagggcg gtgccgtggg caagggccga gcgcatcatg gcggggcccg
      541 gaagaatgag cccgccgcga aagacccatt cccggggatt cgacgcccgt tcgacgtggc
      601 cggattcccc gccgccggcc cattggttcg gtcgtgcgcc gtcgatcgtg tctggcacca
      661 gcgtgtcgat cgtggtggcc gtcccgaacg tggccagcat gacgatgggg gcggggacgc
      721 tggttgcagc gttccgcagg cgtcgcgaca agccgatcaa cgacacccag cggtcggggc
      781 caagctgccc cggcgggtcg taggcgttgc gcacaccccg cgcatggtcg aggctggtga
      841 cccacctgac tgcgcaaccg cgtcggccca gatgggtttc cagcgcctgg gcgcgctcac
      901 ggccggccac attgacgccc acggcgcgcg ccggctgcgc gggcaatcgg tcgagccaat
      961 cggccaggtt ggccgtgtcg tcatgcgcca gggagagtgg cgtggtctca cgctgcccgg
     1021 tggcgggatc cagccagccg aacttgacca gtgtgttgcc ggagtcgatc agaagaatca
     1081 tgggggtcat ttaggccgca ccgatacgtc gccgaccgag atggcgagtt cgcccgctgg
     1141 cgtgcgcaac aggagctgac ccagttcgtt gacgccgcag gcaatgccgg aatggacgac
     1201 ctggccatcg tcgagaatgt tgacgtgctg gccgttgagc gcatcgactt gcgcgaaacg
     1261 cgcggggaat tcgaggagcc cctggcggat ggcccgattg atgccgtcgt accaggcttg
     1321 cgcgatgcgc gagaccagtg cggtggcggg cgattgcgcg acttcctggg ccgcgtcgca
     1381 gatttcgcgc cagtcggcga tctggcgatc gagtgactgg ctgagggagc gcgcgtcgtc
     1441 caggttcagg ccgataccca tgatggccac gtggtggtcg gccgacagcc gcgatgtgcc
     1501 ggcccgcgtg atttcgacca ggatgccgcc aagcttggcg aacttccatt gcaggtcgtt
     1561 gggccatttg agcgtcaggt gtggctgcaa cggcggcggc agcaggccgc gcaacgcctc
     1621 gcaggcggcc acgcccgcga gcggcgacag cgcgggcagc agcctgggtg gcaggaacac
     1681 atcgaacgcg caggaaaaca tgaggttggc ccctgggcgg ttctgccatg tgcgcccggc
     1741 gcggccgcgg ccttgttctt gcagatgcgc ccccaacagc caggggcggt cgagccgcaa
     1801 caggctggtg cgggtcttgg tcagcaagtc ggcgttggtg gacgccgtgc gtgcgaacca
     1861 ttcgacctgg ccaaaatgcg gcaacgccga gcgcagcgcc gtcatgaact ggcccggttc
     1921 gggcaaggga agaggaaggg tcgaagccat ggtgtgtaga atgcgtgaac aggctttgga
     1981 caacagtagc ggtttatccc ggcttgtgcc agttggctgt tcgccctact actatacgtt
     2041 catgccgccc gccgggcgct tccggatcta gtcaaggttc attatatgtt tgctcgcata
     2101 gaaaatcgca cgttccaggg gcgggcgggc gacgtggatt gtctcatcga gtggccggac
     2161 ggccccgcgc ggggctgggc gctggcgctg catccgcatc cgctgcatgg cggcgcgcgt
     2221 gaaaacaaag tcgtcaccac cattgcgcgg gcctgcctgc agcacgggct ggccacggtg
     2281 cggcccaatt tccggggggt cggcaaatca gccggggtgt ttgattcggg ggtgggtgaa
     2341 accgccgaca tgcatgccct ggtcgaacag ttttcccggg catggccgga agaagccgcg
     2401 gggcggtggg tgctggcggg gttttcgttc ggcacgtcgg tggcggcgca actgtattcc
     2461 gtcctggctg aacagggggg cgccacgccc gatgtgttga tgttgctggg gtcggcggtc
     2521 gaacggttcc gtttccgcga cgtggtggtg cccgacgaca cgttcctggt gcatggcgag
     2581 ctcgatgagg ttgtgccgtt ggccgaggcg ctggacttcg cgcgtccccg gtcgctgccg
     2641 gtggtcgtgg tgccaggggc ctcgcacttt ttccatggca agctggtggt gctcaagcaa
     2701 ctgctggcgc agcggctgat ggcgttgggg cacacctgac acgcgcttcc tcgtcggcgg
     2761 gaggggcgcg cgcgcctttt gatcgggaaa tcacctccga tcgcgaaagc actggtggaa
     2821 tcgatgccac ggttatgaca tacatagata tttttagtat caactgtttt cgtatttgac
     2881 ccgccgaact gttacggatg ggttctgctg actcgtccgg tctgcctata atgatcgcca
     2941 tgttttgttt ccggctggtt tctctttctt cttacatgcc caccatttcc tcccctattt
     3001 cgttcctcca tcgcgtgcgg cgcggcattt caatcctcac gttggcggtg gcgccggcgg
     3061 ctgtgctggc ggcaacgccc gccgcgcctg cgcgggtgca gcccgcggca cccgcgcaac
     3121 cggccgcgcc agcccaggcg gcgtccgcca tccccgtgcc cgccgcggtg cgtggcctga
     3181 cgccggtggg tgatctctcc aaggtgccgg cgcccaccat cgatgccaag gcctggctcg
     3241 tgctcgacgc caacagcggc caggtcatca gcgccgtcga ccccgacgaa aagatcgagc
     3301 cggcctcgct gaccaaactg atgtccatct acatcgtctt cgagtcgctg gcgaacaagc
     3361 gcctggcgct ggaccagaag gtcaacgtat ccgagaaggc ctggcgcacc ggcgggtcgc
     3421 gcatgttcat caaggttggc acgcaggtct cggtcgacga tctgctgcaa ggggtgatcg
     3481 tgcagtcggg caacgacgcc accgtggcgc ttgcccaggc ggtcgcgggc agcgaatcgg
     3541 cttttgtgtc cctgatgaac caggaggcac agaaaatggg cctcaccaac acgcattacg
     3601 tcaacgtcga cgggctgccc gaccccgcgc acctcaccac ggtgcgtgat ctggcgacgc
     3661 tcgcacgcaa tctggtcacg cgctttccgc agtacatgca ctacttctcc gagaaagaat
     3721 tcacctacaa caagatcaag cagcgcaatc gcaataggct gctctgggcc gaccccactg
     3781 tcgacggcct caagaccggc cataccgagt cggcggggta ttgcctggtg gcgacggcca
     3841 agcgtgacgg gcgccgcctg gtgtccgtgg tggtcggtgc acccagcgat tcggcgcgca
     3901 ccgaaaccag cctgaaattg ttgaactggg gtttccagaa tttcgacacc atcaagctgt
     3961 acgacaacac ccacgcggct gtccaggcgc gtgtctggga gggcacggtc gaaaccgtgg
     4021 gcctggggca gcctgacgcc acctgggtca ccgttccgcg cggcgagggg cagcaggtca
     4081 agcccatcgc gcagtacacg caaccgctgg tggcgcccct gaagcagggc gcgcgcgtgg
     4141 gcgaggtgtc gctcacgctg gatggcaagg tgttgcgccg cgacccgctt gtcattctca
     4201 aagatgtccc gcaggccggt ttcttcgggc gcatgttcga caaggttcgc ctgatgttca
     4261 agtaggccgc gcgccgacag gccggcaaaa gcgtatcatt ctcagttggt gcggcccgcg
     4321 tgggccgcgc ctttgctttc aggaaggccc ctgaaggcgt gttcccggcc tgtggcccgg
     4381 aatttccccg attcccccga attttgtgtg cgaggcacga aagatgattc ctgatgtcga
     4441 taacgacagc atcgtttatg tgaatggcga acacgtccgc ctgggcgacg ccaagatctc
     4501 cgttctggat cgcggcttca tttttggcga cggcatctac gaagtggtgc cggcctacaa
     4561 cggcaagccg ttccgcatga aagagcatct ggcgcgcctg cagcgcagcc tgggcgccat
     4621 ccgcatcgaa acaggcaaat ccaccgccga ctgggaaaac ctcgtgcacg acatgctcaa
     4681 gcgctctggc ctgggcgact gcatggtgta cctgcaggtc acgcgcggcg tggccaagcg
     4741 cgaccatggc ttcccccaag actgcgaacc cacggtgttc tgcatggtgt cgccgttcaa
     4801 gcgccccgac gcgcacaagc gcgaagacgg tttgtccgtg gtgagcatgc cggattcccg
     4861 ctggctgcac tgtgaaatca agtccgtgtc gctgctgggc aatgtgctgg ccaagcaata
     4921 cgcggtcgac gccggcgtcg acgaagtggt gcagttccgc gacgggcact tgtccgaagg
     4981 ctcttcctgc aacatctggg tcgtgaaaga cggggtgctg ttggcgccgg tgcgtgacca
     5041 ctacatcctt gaaggcatcc gttatgcctt cctggaacag ctcgcggcca aggcgggcgt
     5101 caagttccag tcgcgggtca tcaccaaaca ggaagtcgag gaagccgacg aactgatgtt
     5161 gtcgtcggcc accaaggaag tcctgcccat cacccgctac gaaggcaaac cggtgggcca
     5221 gggcaagccc ggtccggtgt acgccaagct gcgcgcgggt tacgacgagg ccatcgcgcg
     5281 cctctgatca ttctgtcggc gtggagcccg ccatgaaagc aatccctccc gaagaatccc
     5341 tcatcgaata ccccagcgac ttccccatca aggtcatggg caagtcgcat cccgatttcg
     5401 cgcaaaccct gaccgaggtg gtcatgcggt tcgatccggc gttcgacgcg gccacggtcg
     5461 agatccggcc cagcaaggcc ggcaactacc tgagtctgac gttcacggtg cgcgcgacgt
     5521 cgcgcgagca actggacaat ctctatcggg ccttgcacga gcaccccatg gtgtccgtgg
     5581 tgctgtgagc ggccagcccc ccatgatcga cgtctcgtgg ctgccacgcc ccgctcctta
     5641 tcaagaggtg tggcaggcca tgcgtgacat gacggaggcc cgcgacgaca gcacgcatga
     5701 cgcgatctgg ctggtcgagc attcgccggt ctatacattg ggtcaggccg gcaaacccga
     5761 acacatcctg catgccgacg gtatccctgt ggtgcattcc gatcgcggcg gccaggtcac
     5821 ataccatggc cccggccaga tcgtggcgta ttgcctggtc gacctgcgcc gcctgggcgt
     5881 gtacgtgaaa gagtatgtgg tcatgctcga ggacgccgcc atcgccacgc tggccgagct
     5941 cggcgtggcc aacgcctgcc tgaagccggg cgcgccggga gtgtacgtac ccatgccgga
     6001 aggcgatctg gccaagatcg ccgcactggg catcaagatc cgcaacgggc gcggctacca
     6061 cggcctggcg ttgaacgtcg acatggatct gtcaccgttc tctggtataa atccttgcgg
     6121 ctacgagggg ttgcgcaccg tggatctggc gactttcggc gtgcgggtcc cgcttgacca
     6181 ggcggcggaa gtcctgtcgc ggcatctcgt ccgcctattc gagagccatg tggcgcattg
     6241 agtccacact ggctcgttcc agtctgcgca ggccctgtgc cgggaggtct catcatgtcg
     6301 tccgaaaacg cagttttgct cgtcgaccag atcgacggcg tctataccct gaccctcaac
     6361 cggcccggcc agttcaatgc gctgtctgaa gaattgctgg ctgagctgca acgcaatctc
     6421 gatgcgatcg cacgcgacga tgacgcgcgc tgcgtggtgc tggcggccaa cggcaaggct
     6481 ttctgtgccg ggcacgacct gaagcagatg cgcgcccatc ccgaccatga ctactacgcg
     6541 cggctgttcg ccgactgcgg ccgggtcatg caaagcctgg tcgagctgcc ggtgccggtg
     6601 atcgcgaagg tacagggcat cgccaccgcg gcgggctgcc agctcgtggc gagttgtgat
     6661 ctggctgtgg cggtcgacac cgcgcgcttt gccgtatcgg gcatcaacgt tgggttgttc
     6721 tgcgccacgc cggcggtggc gctgacgcgc aatgtgccca tgaagaaggc gttcgagatg
     6781 ctgatcacgg gtcgcttcat cagcagcgcc gaggccgtgg accacgggtt gatcaaccag
     6841 gcggtggatg ccgagcggct cgacagcgtg gtggccgcgc tggtgcagac gatctgcgcg
     6901 aagagccccc tggcggttcg cacgggtaag ggcatgtacc ggcgccaaca cggcatggcg
     6961 ctggccgatg cctacgacta tgccggccgc atcatggcgc gcaacatgat gaccgaggat
     7021 gtttgcgagg ggatcgatgc cttcacgcag aagcgcgcgc cggtctggaa gggtcgatag
     7081 tgtgctgtcc ggttgagtgg gttacccggt ctcagtcggc ttcggcgcca gcctatgagg
     7141 cttagcggtt gtttggtcca agtgttttta ttgggttcga tgctgtgggt atctgaagac
     7201 gctgtctctc ggggcctggg gcaagcccgg cacggcgtgc ttcatccgga tccgtctcgt
     7261 ccaggcgggc gtcgggcgaa actcgcctcg caaaccccgc gaggctcaga cagcgcccga
     7321 cgaagtcccc cgccttcccg agccagctcc ggcgcacgcc ttacgccggg cttgccccag
     7381 gccccgagag acagctcacg gtgacggcat gcgggggagc cggcaaaccc gccgttaggc
     7441 aaagagcgtg tcatcgcgga ggcctcgagt cggcatatgg gccgtgctca atcgtccaca
     7501 acctcggtat atatccttcc gagcatgttc gttgtcgtag ccccagggtg ggagaggtgg
     7561 tgcaggccgg cgtttcggcc ggcgccgggt ctggcttggc caggcggggg cagtcgtcgg
     7621 gcggtgtttg agcgcagcct cgcgatagcg aggcgtagcg agttcagccc gacgcccgcc
     7681 tggacgaggc aggcccgggc agtcggggcg cgcagc
//
LOCUS       DASTNK010000071         9030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1348400, whole genome shotgun sequence.
ACCESSION   DASTNK010000071 DASTNK010000000
VERSION     DASTNK010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9030
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348400"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..351
                     /locus_tag="VFR20_05545"
     CDS             46..351
                     /locus_tag="VFR20_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU0230016.1"
                     /translation="MGSESKALLWRVAECDRPRCDYFLDGEAHSALKGDTVLTAILTT
                     SAMLRRSEFGGGPRAGFCLMGACQDCWVWLEDGTRIRACSTFVEPGMRVVTCASVRP"
     gene            348..1796
                     /locus_tag="VFR20_05550"
     CDS             348..1796
                     /locus_tag="VFR20_05550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEU0230017.1"
                     /translation="MTAAIEPVIVGAGPAGLRAAQALADAGLAPVLIDESPKVGGQIY
                     RQTPDGMTRDHRALYGFEHGKARAVHGILDKLAGRVDYRPGTVVWNAEGRTLYLQSGG
                     LIGGVSFTHLILATGATDRVLPFPGWVTPGVYTLGGAQVSLKYQGCAIGRRVVFAGTG
                     PLLYLVAYQYAKAGARVVAVLDTAKVSDQAKALPGLIRAPALLAKGLWYAGWLRAHGV
                     PVRTGVRLLEARGAERVESFVWERHAGDADSVRETACDAVGFGYALRAETQLASLLGC
                     EFQFDERDRAWLPVRDDVGRASRPGVYLVGDGRGIAGADAAEISGELAALALLSDVGM
                     VSDPVRTHLLTARARRVEAVRDTLEKIFPFPRDWAARADDDLVVCRCEEITAGQIRRA
                     VREDGAHEMNRVKALTRAGMGRCQSRMCGLAAAEIVACEAGLNVSEVGRVRSQPPVKP
                     LIIGAFEPARAEGKPMSHPGSRASRTGGDARG"
     gene            1789..2952
                     /locus_tag="VFR20_05555"
     CDS             1789..2952
                     /locus_tag="VFR20_05555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0230018.1"
                     /translation="MAETTRTDVAIVGGGIVGASAALFLRGLGYSVVLLESGLCGSKA
                     SGVNYGGVRRQGRQPDQLPLSLRAHALWSTIRQLAGMDGEYVRSGHLKLARNAEDLAA
                     LESYLALTRDFGLGLRMVTGAEFRKRFPWLGGVVIGGSLCPEDGHANPRLVSPALARA
                     ARAAGADIREQTRVIHAEKDGDEFVIACEGGLTVRSSRLLNCAGAWGHVIAEMFGRTV
                     PEHAIHPVMFVTEPLPVFMNVSLGIQGGGIYARQVERGNCVIGGGRGYPLDNDFARPG
                     AAAMSDLLRETTGVIPALRGAQVIRFWSGYEGSFDDDNPVIGPDGVVPGLFHAFGFCG
                     AGFQLGPAVGEVLADLVHSGHARVPIDAFSIQRFETQTRPSSSPFPKIVEHQG"
     gene            2961..4028
                     /locus_tag="VFR20_05560"
     CDS             2961..4028
                     /locus_tag="VFR20_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0230019.1"
                     /translation="MTKRYFPHSLKTGALLGVALFSAWAATAHADQTTLYIGMNGGDM
                     ERAFTQHVFPPFEKAHNVKVVVVPGTSADVLAKAQAYKAHPQMHVMFLDDGVMYRAIS
                     MGLCEKMNPSPELSQLYPTARMKDGMAAAVDMGLTGVGYNTKMFKEKHWAPPTSWGDF
                     ANPKYKGKVVMQSMSSSTYGLHAFLMFNRIEGGSAKNVNPGFSKWNDTIGKNVLEYIS
                     NSSKISEMVQTGEAGIFPLTFTAIARLAKRGQPVAFAVPKEGPVLLSVGECVIAHNTQ
                     PKLAQELARYLLTPKAQELALKYSGNVPSNPNAKGGDADQQKYLDDFKKALKHAVVLD
                     WDVINKNRPEWNVRWNRTVEQ"
     gene            4174..5280
                     /locus_tag="VFR20_05565"
     CDS             4174..5280
                     /locus_tag="VFR20_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEU0230020.1"
                     /translation="MKTRAAVAWKAGAPLTIEDVELDGPKAGEVLIEVKATGICHTDY
                     YTLSGADPEGLFPAILGHEGAGVVLETGAGVSGLKPGDHVIPLYTPECRQCKFCLSRK
                     TNLCQAIRATQGKGLMPDGTGRFKVGGKFVHHYMGTSTFSNHIVVPEIAVAKIRDDAP
                     FDKVCYIGCGVTTGVGAVIFTAQVEAGANVVVFGLGGIGLNVIQGAKMVGAGKIIGVD
                     LNPEREALARKFGMTHFVNPKDVDNVVDHIVSLTDGGADYSFECIGNVKVMRQALECC
                     HKGWGQSIIIGVAEAGAEISTRPFQLVTGRHWKGSAFGGARGRTDVPKIVDWYMEGRL
                     NIDDLITHTLPLEKINDGFDLMHRGESIRSVVVY"
     gene            5280..6131
                     /gene="fghA"
                     /locus_tag="VFR20_05570"
     CDS             5280..6131
                     /gene="fghA"
                     /locus_tag="VFR20_05570"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEU0230021.1"
                     /translation="MPDGLELISEHRCFDGVQRFYRHDSRAIGLPMRFSVFQPPQAVA
                     GRVPVLFFLAGLTATEENFMVKAGAQRLAAQHGLMLVSPDTSPRGAGVPGEGDDWDFG
                     TGAGFYLDATQAPWKTHYRMESYVARELFDIATGELPGDAGRVGIFGHSMGGHGALVL
                     ALRHRDQYRSVSAFAPIAAPSRCPWGQKAFTGYLGDDHAAWAEHDASLLMERSSCPYP
                     GGILVDQGLADAFLDEQLYPGEFEAACAKAGQPLTLRRHEGYDHGYYFLSTFMEDHLA
                     FHAERLK"
     gene            complement(6220..7140)
                     /locus_tag="VFR20_05575"
     CDS             complement(6220..7140)
                     /locus_tag="VFR20_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0230022.1"
                     /translation="MQDIDTKTLRIFVAVCDMQNMARAAEHEHIEPSAVSKRIAQMEA
                     TLGTPLLLRSRRGVRPTAAGIAVLEHARGVLFTMDRIAHDAATFASGGVGHVRLIATA
                     SAVAEQLFDDIASFMRAPENQNIQVEVEERLSRDLVRQIRSGMASLGVCWNNLDFEGL
                     QQQPYREDHLAAAVHPGHPLARNASLVFEQTLDYEHVGLPPSTAVHTMLQQLAARKDR
                     RLSYRVIVSNFDAAFRVVAANLAISIVPSEVGESYKNTLGIKIIPLRDVWATRRFAVC
                     YQTIGTLQPAARRMVQHLVGCATRECHAEG"
     gene            7278..8234
                     /locus_tag="VFR20_05580"
     CDS             7278..8234
                     /locus_tag="VFR20_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011295996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEU0230023.1"
                     /translation="MAGLPSRVVMREVGLRDGLQSIRTILPTHRKLEWVRKAYEAGQR
                     EIEAGSFVPPRLLPQLADTADVVSFAKTLPGLSVSVLVPNMKGAERAVSAEVDLMLIP
                     LSASVAHSRANLRKTPDEVIAEVERIRAMRDAAGSKTLIEGGVGTAFGCTLQGKVEPA
                     EVLRCMQALLDAGVDRVSLADTVGYADPRAVRALFEQARDIAGDRFWCGHFHDTRGLG
                     LANVYAALETGVTRFDASLAGIGGCPHAPGASGNVASEDLAFMLGSMGVETGVDIGAL
                     LALRGTVAGWLAGEALHGVIARSGLPLTFPFPFSSDRAAALS"
     gene            8231..>9030
                     /locus_tag="VFR20_05585"
     CDS             8231..>9030
                     /locus_tag="VFR20_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEU0230024.1"
                     /translation="MTLQSPVHLPLEGVRVIEFTHMVMGPTCGMILADLGAEVIKIEP
                     PGGDKTRNLPGLGIGFFRTFNRNKKSVVLDLHEEAGRATALELIGRCDVMLENFRPGM
                     MASIGLGYDDLSRRFPSLIYVSHKGFLPGPYENRLALDEVVQMMGGLAYMTGPRGKPL
                     RAGTSVNDIMGGMFGAIGVLAALREREHTGRGQEVQSALFENCVFLAAQHMQQFSMTG
                     EPPPPMPERVSAWSVYDVFNLAGGEQLFIGAVSDRQFATLCQVIGAPGL"
BASE COUNT         1557 a         2594 c         3083 g         1796 t
ORIGIN      
        1 gtggcgcctt gagcgcatgg catggaaaca agtaaagaga agcgaatggg cagtgaatcc
       61 aaggcgttgc tatggcgcgt ggctgaatgc gatcgaccgc gttgcgacta ttttctcgat
      121 ggtgaggcgc acagcgcgct gaaaggcgat accgtgctga cggcgattct gaccacaagc
      181 gcgatgctgc gtcgatcgga attcggcggt gggccgcgcg cgggcttttg cctgatgggg
      241 gcatgccagg actgctgggt atggctggaa gacggcacgc gcatccgggc ctgttccacg
      301 ttcgtggagc ccggcatgcg tgtagtgacg tgcgcatcgg tgcgcccatg accgcggcca
      361 tcgaaccggt gatcgtgggt gccggtccgg cgggcctgcg tgccgcccag gcgttggccg
      421 acgcggggct ggcccctgtg ctgatcgacg aatctccgaa ggttggcggc cagatatatc
      481 gccagactcc cgatggcatg acacgcgatc atagggcgct atatggattt gagcatggca
      541 aggcgcgcgc tgtgcatggg attctcgaca agctggcggg gcgggtcgat tatcgtcccg
      601 gcacggtcgt ctggaatgcg gaagggcgaa ccttgtatct tcaatccggc gggttgatcg
      661 gaggcgtctc ctttacccac ctcattctcg cgacaggcgc caccgatcgt gttctgccgt
      721 ttcccgggtg ggtgacgccg ggggtttata cgcttggtgg tgcgcaggtg tcgttgaagt
      781 accaggggtg cgccattggt cgccgggtcg tttttgccgg aaccgggcct ttgctgtacc
      841 tggtggccta tcagtatgcg aaggcgggcg cgcgtgtggt ggctgtgctc gacaccgcga
      901 aggtatccga tcaggccaag gcattgcctg ggctgatcag ggcgccggcg ttgctggcca
      961 aggggctttg gtatgccggt tggctacggg cacacggtgt tccggtgcgc acgggtgtgc
     1021 gcttgctcga agcccgtggc gcggagcgtg tggaatcctt tgtctgggag cgccacgccg
     1081 gcgacgccga ttcggtccgc gaaaccgcct gcgatgcggt ggggttcggc tatgccttgc
     1141 gtgccgaaac acaacttgca agcctgttgg gctgcgaatt ccagttcgat gagcgtgacc
     1201 gcgcctggct gcctgtccgc gacgatgtgg gtcgcgcgtc gcgcccgggg gtgtatctgg
     1261 tgggcgacgg gcggggtatc gcgggcgcgg acgccgccga gatcagtggt gaactggccg
     1321 cgttggcctt gctgtccgac gtggggatgg tgtccgatcc cgtacgcaca cacttgctga
     1381 ccgcgcgtgc ccggcgtgtg gaggccgtgc gtgacacgtt ggagaaaatc tttccgttcc
     1441 cgcgcgactg ggccgctcgg gccgatgatg atctggtcgt ctgtcgctgc gaggagatca
     1501 cggctggcca gatacgccgc gctgttcgag aggacggggc gcatgagatg aatcgggtca
     1561 aagccctgac gcgcgccggc atggggcgct gccagagtcg tatgtgtggg ttggccgccg
     1621 ccgagatcgt ggcttgcgag gcggggttga atgtcagcga ggtggggcgg gtccgtagcc
     1681 aaccgcccgt caagccgttg atcatcggcg cgttcgaacc cgcccgggcg gaggggaaac
     1741 cgatgagcca tccgggttcg cgcgccagcc gaaccggggg agacgcccgt ggctgaaacg
     1801 acgagaaccg acgtggccat tgttggcggc ggcattgtgg gtgccagcgc ggccttgttc
     1861 ctgcgcggat tgggatattc ggtggttttg ctggagtcgg ggctttgcgg gtcgaaggcc
     1921 agcggcgtga actacggcgg cgtgcgccgc cagggacggc agccggatca gttgccattg
     1981 agcctgcgcg cgcacgcatt gtggagcacg atccgtcagc ttgccgggat ggatggcgaa
     2041 tatgtgcgct ccggccatct gaagctcgcg cgcaacgccg aggatttggc ggctctggag
     2101 tcttacctgg cgttgacgcg cgatttcggt ctgggcctgc gcatggtgac cggggctgag
     2161 ttccggaaac ggttcccctg gctgggcggc gtggtcattg gaggctcgtt gtgtccggaa
     2221 gacgggcatg ccaatccccg gttggtgtcg ccggcgttgg cccgggcggc gcgtgccgct
     2281 ggcgccgata ttcgtgagca gacgcgtgtc atccatgccg agaaggatgg cgatgaattc
     2341 gtgatcgcct gcgaaggtgg tctgacggta cggtcgtccc gcctgctgaa ctgcgcggga
     2401 gcctgggggc atgtgattgc ggagatgttc ggccggacag tgccggagca cgcgatccat
     2461 cccgtcatgt tcgtgacgga gccactaccg gttttcatga acgtcagcct gggcattcaa
     2521 gggggtggca tttatgcgcg ccaggtggaa cggggcaatt gcgtgatagg tggtggccgc
     2581 ggctaccccc tggacaacga cttcgcgcgc ccgggcgccg ccgccatgag cgatttgttg
     2641 cgggagacga cgggtgtgat ccccgctctg cgcggcgcac aggtgattcg gttctggagc
     2701 ggctacgaag gcagcttcga cgacgataac ccggtcattg gtccggacgg tgtcgtgcct
     2761 ggactgttcc atgccttcgg cttctgtggc gccggctttc aacttggccc cgccgtcggc
     2821 gaggtactgg ccgacctggt gcattcgggc cacgcccggg tgcccatcga tgccttcagc
     2881 attcagcgat ttgagacgca gactcggccg tcctcgtcgc cttttcccaa gattgtcgag
     2941 caccaaggat aggagtagac atgacaaaac gatatttccc ccattcgttg aaaaccggcg
     3001 ccttgttggg cgttgccctg ttctctgcgt gggcggccac cgcccatgcg gaccagacca
     3061 cgctgtacat cggcatgaac gggggcgata tggagcgtgc cttcacgcag cacgtattcc
     3121 cgccgtttga gaaagcccac aacgtcaagg tcgtcgtggt gcccggcacc tcggccgacg
     3181 tgctggccaa ggcccaggcc tacaaggcgc atccccagat gcatgtgatg ttcctggacg
     3241 atggggtcat gtatcgcgcc atttcgatgg gtttgtgcga gaagatgaac ccgtcgccgg
     3301 aattgtcgca gctgtatccc acggcgcgca tgaaggacgg catggcggcc gccgtcgaca
     3361 tggggctgac cggtgttggc tacaacacca agatgttcaa agagaagcat tgggcgccac
     3421 ccacgtcgtg gggcgatttc gccaatccca agtacaaggg aaaagtggtg atgcagtcca
     3481 tgtcgagcag cacttacggc ctgcacgcct tcctgatgtt caatcgcatc gagggcggct
     3541 cggcaaaaaa tgtgaatccc gggttctcaa aatggaatga cacgattggc aagaatgtat
     3601 tggagtacat ctccaattcg tccaagatct ccgaaatggt gcagacgggt gaggcgggca
     3661 tcttcccgtt gaccttcacg gccatcgcgc gtctggccaa gcgtggccaa cccgtcgcct
     3721 tcgccgtgcc gaaggagggg ccggtcctgc tctcggttgg cgagtgcgtg atcgcccaca
     3781 acacacagcc aaagctggcc caggagcttg ctcgctactt gctgaccccc aaggcgcagg
     3841 aactggccct gaaatacagc ggcaacgtgc cgtccaatcc caatgccaag ggtggggacg
     3901 ccgaccagca aaagtatctg gatgatttca agaaggccct gaagcatgcc gtggtgctgg
     3961 actgggacgt gatcaacaag aaccggcctg aatggaacgt gcgctggaac cggacggtcg
     4021 agcagtaatc cgctggccgg ggtcgggcaa tggggaaatg cccgacccct ttcaagcaat
     4081 ggcacggttc ccggggtctt cgaatattga gccgttgtgc ttgtcggcta tcattggctg
     4141 attcaatttc gctgatctca aaaggagtct tgcatgaaaa cacgagccgc agtcgcatgg
     4201 aaggcgggtg cgccgttgac gatcgaagat gtcgagctcg acggtcccaa ggccggtgaa
     4261 gtactcatcg aggtcaaggc caccggcatt tgccacaccg attactacac gctatcgggc
     4321 gccgacccgg aaggcttgtt cccggccatc cttgggcacg aaggcgccgg cgtggtgctg
     4381 gaaacggggg cgggcgtcag tgggctcaag ccgggtgatc acgtcatccc gctctacacg
     4441 ccagaatgcc gacaatgcaa gttctgcctg tcgcgcaaga ctaacctgtg ccaggcgata
     4501 cgcgccacgc agggcaaggg gctcatgcct gatggcacgg gccgtttcaa ggtgggcggc
     4561 aagttcgtgc atcactacat gggcacgtcc acgttctcca atcatatcgt ggtgcccgag
     4621 atcgccgtgg ccaagatccg cgacgacgcg cctttcgaca aggtctgcta catcggctgt
     4681 ggtgtcacca ccggtgtagg cgccgtgatc ttcacggccc aggtcgaagc cggcgccaac
     4741 gtggtcgtgt tcggcctggg cggcatcgga ttgaacgtca tccagggcgc gaagatggtg
     4801 ggcgcgggca agatcatcgg ggtggatctg aatcccgagc gtgaggcgct ggcgcgcaag
     4861 ttcggcatga cgcacttcgt caaccccaag gatgtcgata acgtggtcga ccacatcgtg
     4921 tcgctgactg atggtggcgc cgattattcg ttcgagtgca taggcaacgt caaggtcatg
     4981 cgccaggcgc tggaatgctg ccacaagggc tgggggcaat ccatcatcat tggtgtggcc
     5041 gaggccggcg ctgaaatctc cacccgcccc tttcagcttg tgacggggcg tcactggaag
     5101 ggctcggcgt ttggcggcgc gcgtgggcgc accgacgtgc ccaagatcgt cgactggtac
     5161 atggagggcc gtctcaatat tgatgacctg atcacacaca ccttgccgct cgaaaagatc
     5221 aatgacgggt tcgacctgat gcatcgcggt gagtccattc gttccgtggt ggtgtattga
     5281 tgccggacgg acttgagctg atcagcgagc accgctgttt cgatggtgtc cagcgttttt
     5341 atcggcatga ttcacgtgcc attggtctgc ccatgcgatt ctcggtgttt caaccgcccc
     5401 aggcggtggc cgggcgcgtg ccggtgctgt tcttcctggc ggggctcacc gccaccgaag
     5461 agaacttcat ggtcaaggcg ggtgcgcaac gcctcgccgc gcagcatggg ttgatgctgg
     5521 tctcacccga caccagccct cgtggggccg gggtgcccgg tgaaggcgat gactgggact
     5581 tcggcacggg cgcggggttc tatctggacg cgacgcaagc cccgtggaag acgcattacc
     5641 gcatggaaag ctatgtggcg cgtgaactgt tcgacattgc caccggtgag ttgccgggtg
     5701 atgcgggccg ggtcggcatt tttggccatt cgatgggcgg ccatggcgcg ctggtgctgg
     5761 cgctgcgcca ccgggatcaa tatcggtccg tgtcggcgtt cgcgcccatt gccgccccat
     5821 cgcgttgccc atgggggcaa aaggcgttca cgggctatct gggtgatgat catgctgcct
     5881 gggccgagca cgacgcttcg ctattgatgg aacgatccag ttgcccgtat ccgggcggca
     5941 ttctggtcga ccaggggttg gccgatgcct ttctcgatga gcaactctac cccggcgaat
     6001 tcgaagcggc atgcgccaaa gccgggcagc cactcacgct gcgccggcac gaaggctatg
     6061 accacgggta ttattttctg tcgaccttca tggaagatca tttggccttt catgcggagc
     6121 gcttgaaata gccaccaccc acgccctggc gtgagaatgg tcagcgggcc gggcattgtc
     6181 gcgcgcgtgt ggccactccg atcctcacga gcagggcggc tacccctccg cgtggcactc
     6241 cctggtcgcg caaccgacca ggtgttgcac catccgccgc gcggcgggct gcaaagtgcc
     6301 gatcgtctga tagcacacgg cgaagcggcg cgtggcccag acgtctcgca atgggatgat
     6361 tttgatccca agcgtgtttt tgtacgattc cccgacttcc gacgggacga tgctgatggc
     6421 gaggttggcg gccacgacac ggaacgccgc gtcgaagttc gacacgatca cccggtaaga
     6481 caggcgccgg tccttgcggg cggcgagctg ttgcagcatc gtgtgcacgg cggtcgacgg
     6541 aggaaggccc acgtgttcat agtccagggt ttgctcgaag acgagtgacg cgttccgtgc
     6601 cagcgggtgc cctgggtgga cagccgccgc gagatggtct tcgcggtatg gttgctgttg
     6661 caggccctcg aaatccaggt tgttccaaca cacccccaat gaggccatgc cgctgcgtat
     6721 ctgccgcacc agatcgcgtg acaggcgctc ttcgacttcg acctggatat tctggttctc
     6781 gggtgcccgc atgaacgacg cgatgtcgtc gaacaactgc tcggcgacgg cggacgcggt
     6841 ggcgatcagt ctgacatgcc cgacgccgcc gctggcgaat gtggcggcat catgggcgat
     6901 ccggtccatc gtgaacagaa cgccacgcgc gtgttcgagc accgcgatgc ctgccgctgt
     6961 tgggcgcacc ccccgccgtg aacggagcag aagaggggtg ccaagtgttg cttccatctg
     7021 tgcgatgcgc ttgctcacgg ccgatggttc tatgtgttcg tgttccgcgg cgcgcgccat
     7081 attctgcatg tcacagacgg ccacaaagat ccgcaaggtt ttcgtatcga tgtcttgcat
     7141 ggccacagcc tctattagta tttccatatt ggaacttatc aagttccaaa taaccactgt
     7201 acagcattta tggaacgtct aaagttgata ctacggcaac gccgacagat tattggcgca
     7261 acgaaaaagg aatcgatatg gcgggactcc cttcccgtgt tgtgatgcgc gaggtcgggc
     7321 tgcgcgacgg cctgcagagc atccgcacca ttcttccgac ccaccgcaag ctcgagtggg
     7381 tccgcaaggc ctatgaggcc ggacagcggg agatcgaggc ggggtctttt gtgccccccc
     7441 gtttgctgcc gcaattggcc gataccgctg acgtcgtgag tttcgccaag accctgccgg
     7501 gtctgtcggt ctcggtgctg gtgccgaaca tgaaaggggc cgagcgcgcg gtgtcggcgg
     7561 aggtggatct gatgttgatt ccgctgtccg ccagtgtcgc gcacagtcgg gccaatttgc
     7621 gcaagacgcc cgatgaggtc atcgccgagg tggagcggat ccgtgcaatg cgcgatgcgg
     7681 ccggatcgaa gactctgatc gaagggggcg tcggcacggc gttcggctgc accctgcagg
     7741 gcaaagtcga acctgcagag gtgttgcgct gcatgcaggc gttgctcgat gcgggcgtgg
     7801 atcgcgtcag cctggccgac acggtcggtt atgccgatcc gcgcgccgtg cgtgccttgt
     7861 tcgaacaggc gcgcgacatc gccggagacc ggttctggtg cgggcatttt catgacacgc
     7921 gcgggctggg tctggccaat gtctatgcgg cgctggaaac cggtgtgacg cgcttcgacg
     7981 catcgctggc cggaatcggc gggtgccctc acgcgcctgg cgccagtgga aacgtcgcca
     8041 gtgaagatct ggcgttcatg ctggggagca tgggcgtcga gacgggggtc gatatcggcg
     8101 cgctgctggc gctgcgcggc acggttgccg ggtggctggc cggagaggcg ctgcatggtg
     8161 tcatcgcgcg gtccggactt cccctcactt tccctttccc attttcctcg gatcgagcgg
     8221 cggcgctgtc atgacactcc aatcacctgt tcatctgccg ctggaaggcg tgcgcgtcat
     8281 cgaattcact cacatggtca tgggtcccac ctgcgggatg atcctggctg atctcggcgc
     8341 ggaagtgatc aagatcgaac ccccgggagg cgacaagacg cggaatctcc ccgggctggg
     8401 catcggtttc tttcgcacct tcaatcgaaa caaaaagagt gtcgtgctgg atctccatga
     8461 agaagcgggg cgcgccacgg cattggagct gatagggcga tgcgatgtga tgctcgagaa
     8521 tttccgtccg gggatgatgg catccatcgg attgggctac gacgatctgt cgaggcgatt
     8581 tccgagcctg atctacgtgt cgcacaaggg cttcctgccc ggaccgtacg agaaccgcct
     8641 ggcgctcgat gaagtggtgc agatgatggg ggggctcgcg tacatgacgg gcccgcgagg
     8701 caagcctttg cgggcgggca cctcggtgaa cgacatcatg ggaggcatgt tcggtgcgat
     8761 cggcgtgctt gcggcgctgc gtgagcgcga gcatacgggg cgcggacagg aggtgcagag
     8821 cgccttgttc gagaactgtg tttttctggc ggcccagcat atgcagcaat tctcgatgac
     8881 cggtgagcca ccgccgccca tgccggagcg agtctcggca tggagcgttt atgacgtgtt
     8941 caatctggcg ggcggcgagc agctgttcat cggcgccgtg agcgaccggc aattcgccac
     9001 gttgtgccag gtgatcgggg cgcccgggct
//
LOCUS       DASTNK010000072         9391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_791996, whole genome shotgun sequence.
ACCESSION   DASTNK010000072 DASTNK010000000
VERSION     DASTNK010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9391
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791996"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VFR20_05590"
     CDS             <1..422
                     /locus_tag="VFR20_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEU0230025.1"
                     /translation="IAEHWRGWLAYGLHGLVSPGFGLLLAGLVVAWYCYLIDTRVPEA
                     LSKRFSGLYTLLVNKYYDDWFNEQVVARGTRALGRGLWQGGDRGVIDGIVNGSARLIG
                     WTASVGRLMQSGFIYHYAFAMIIGIMALLTFFVLTVH"
     gene            434..1921
                     /locus_tag="VFR20_05595"
     CDS             434..1921
                     /locus_tag="VFR20_05595"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEU0230026.1"
                     /translation="MASSTLPWLTLSIFVPIIAGLLVLALGSDKRPGFTRKLALVGAV
                     LGFLVTLPLYTGFNPNTANMQFVENVPWITSLAINYHLGVDGISLWFVLLTSFITVIV
                     VLAGWESVTHRVAQYMAAFLILSGFMIGVFAALDGMLFYIFFEATLIPMYIIIGAWGG
                     PNRVYAAFKFFLYTLLGSLLTLIAFIYLWHASGGSFDILTWQNTRLGYTAQILIFVAL
                     MASFAVKVPMWPVHTWLPDAHVEAPTGGSVVLAAIMLKLGAYGYLRFSLPIAPDASHS
                     LAGLIITLSLVAVIYIGLVAIVQDDMKKLVAYSSVAHMGFVTLGFFIFNTAGVEGAIM
                     QMISHGFVSGAMFLCIGVMYDRLHTRRIADYGGVINMMPRFVAFFVLFSMANCGLPGT
                     SGFVGEFTVIMGAVQHNFWIGLLAATALIWGAGYSLWMLKRVAYGAVGNNKVGTMKDL
                     NKREFLIMGIMAIAVLYMGIYPKPFSDVMHSSVQALLHHVSISKL"
     gene            1931..3418
                     /gene="nuoN"
                     /locus_tag="VFR20_05600"
     CDS             1931..3418
                     /gene="nuoN"
                     /locus_tag="VFR20_05600"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEU0230027.1"
                     /translation="MMQSSFHIALATPEILLLILATLVLLVDGFGKSVQRRTTFALTL
                     VSLLILTLVSLWQWSEGVHGSAFGGLYVVDPLSHLLKIATYLAVALILVYGRDYAEEH
                     EIMLTGGEFYSLTLMTLLGQMVMISAGNMITVYLGIELMSFPLYALIALRRDNVAATE
                     AAMKYFVLGALGTGMLLYGMSMIYGATGQLGLQQIAHVIISGGAHRLPVVFGTVFVIS
                     AFAFKLGNAPFHMWVPDVYQGSPTSVTLVLSTTPYLAAFAAMVRMVMLGLHGVALDWQ
                     PMLLILAVLSLAVGNIVAIAQTNFKRMLAYSMIAHMGYVFLGLLSGVVANRPAALEQA
                     YGASLFYLLIYVFSTSLTFGLIVIMTRKGIECDEISDLKGLNRRNPVLAGALLMAMFS
                     LAGIPPLAGFYAKLSVLQVLVGADHVTIAVVAVMLSLIGAFYYLRVVKVAYFEDPEGE
                     VITGSAGTVANGLLSLNGLLLLGLGVLPGGLMALCIKAIGSSLGL"
     gene            3512..3607
                     /locus_tag="VFR20_05605"
                     /pseudo
     CDS             3512..3607
                     /locus_tag="VFR20_05605"
                     /inference="COORDINATES: protein motif:HMM:NF022446.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 domain-containing protein"
     gene            3845..4045
                     /locus_tag="VFR20_05610"
                     /pseudo
     CDS             3845..4045
                     /locus_tag="VFR20_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
     gene            4477..6639
                     /gene="ovoA"
                     /locus_tag="VFR20_05615"
     CDS             4477..6639
                     /gene="ovoA"
                     /locus_tag="VFR20_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-histidylcysteine sulfoxide synthase"
                     /protein_id="HEU0230028.1"
                     /translation="MHSISSIAPKNPSPQECSEPALMPRTPNLADPDVNRLRATLREY
                     FTATFDRYESLFECLANDEAFVVKSISLRHPLIFYFGHTATFFVNKLLLARLIDQRID
                     ARLESIFAVGVDEMSWDDLDDANYEWPSVDEVRRYRDKVRALVTRLIDKTPLQSPIGW
                     DSPWWAIVMGIEHERIHLETSSVLIRQHALQYVRPSENWVALNAEAGSPPDNDLVRVH
                     GGMVRVGKALSSAQYGWDNEYGRHEEYVNDFQASRYLVSNAEFLLFVEAGGYQNPDYW
                     EEEGRKWLAFSQVTHPTFWVPHGEAWRLRLMTEEAPMPWDWPVETNYHEAKAFCNWKK
                     AETGQSVRLPTEDEWYRLYDVAGLSDPGESRVAANLQLDHWASSCPVDRFRQGQFYDV
                     VGNVWQWTETPTYPFDGFDTHPWYDDFTTPTFDGRHNLIKGGSWISCGNEAVRASRYA
                     FRRHFFQHAGFRYVVSDETPVNLSPYYETDKLMSEYAEFHYGAEYFGVPNFSRSLVEL
                     ARPFLEGRPRVNALDLGCASGRASFELARHFDHVTGVDFSARFIEQGVLLAGQGVLRY
                     TLADEGELVSYCASSLAELGLDDVRNKVAFFQGDACNLKPQYAGYDFVLAANLIDRLY
                     SPAKFLDAIHERIVPGGVLMIASPYTWLVEHTPREAWIGGFKKDGESYTTLDGLKAHL
                     GAHFRLLHGPAEVPFVIRETKRKFQHTLSEVTLWERVR"
     gene            6909..8369
                     /locus_tag="VFR20_05620"
     CDS             6909..8369
                     /locus_tag="VFR20_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEU0230029.1"
                     /translation="MRWFFLCGMSWLLGGAVSPAFGQARAAVASHEVVVLKVDGIISP
                     ASSDFILAGIDRATHDNAQLVVIELDTPGGLDSSMRTIVQHILASPVPVATFVSPAGA
                     RAASAGTFILYASHIAAMTPASNLGAASPVTIGLGGNDEAPAKPSASSRPSSSPAGQR
                     SDSSVGQQASTQPQLSEGDTMKKKIMNDAVAYIRSLAQLRGRNADFAEKAVRDAASLS
                     APEALKDGVIDLIADNLDQLLTKLDGRQVKLGSGRVVTLATSQPDIVVLSPDWRTRVL
                     GFIADPQIALILLMAGVFGLFYEAMNPGLAVPGVAGLISLLLGLYAFQMLPVNWVGVG
                     LIALGTALMIAEAFMPTFGVVGAGGVVAFVLGGIFLTDTTIPGYGLSVPFLIGIAVAC
                     AILFFIVGSVAARAHRRRVVTGAEEMRGLRGVVTSVNGAVVYAEVRGERWKVRSPQAL
                     APGDLVRVVSLNGLTLLVEPADDAHSMKDKGEHCVL"
     gene            8359..9189
                     /locus_tag="VFR20_05625"
     CDS             8359..9189
                     /locus_tag="VFR20_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEU0230030.1"
                     /translation="MSYSYDIVVIAVVIILALVLINAVRILREYQRAVVFTLGRFSGV
                     KGPGLIFVIPVVQQIVRVDLRVVTMDVPSQDVISRDNVSVKVDAVLYFRVMAPDKAII
                     QVERYLEATSQLAQTTLRAVLGKHDLDDMLSEREKLNLDVQEILDAQTDSWGIKVTNV
                     EIKRIDLNENMIRAIARQAEAERERRAKVIHAEGEKQAAQALMEAAETLAKQPSAMQL
                     RYLQTLTQVAGDKSSTLVFPIPVDLLSALMNNKVGGPGASLLQYPPEGPAGAPDKPGS
                     "
BASE COUNT         1577 a         2862 c         3013 g         1939 t
ORIGIN      
        1 agatcgcgga gcactggcgc ggctggctgg cctacgggct gcacggcctg gtttcaccgg
       61 gattcgggct gctgctcgcg gggctggtgg tggcgtggta ctgctacctc atcgacacac
      121 gcgtaccgga ggcgctgagc aagcgcttct cgggcctgta tacgctgctg gtcaacaagt
      181 actacgacga ctggttcaac gagcaggtgg tggcgcgcgg cacgcgcgcg ctcgggcgcg
      241 gtttgtggca gggcggtgat cgcggcgtga tcgacggtat cgtcaacggc agtgcgcggc
      301 tgatcggctg gacggcttca gtgggtcgac tgatgcagag cggattcatc tatcactacg
      361 cgtttgcgat gatcatcggc atcatggctc tccttacctt tttcgtgctg acggttcact
      421 gatggctggc gatatggctt cttctactct cccctggctg acgctctcga tcttcgtgcc
      481 gatcatcgcg ggcctgctcg tgctcgcgct gggcagcgac aaacggcccg gtttcacgcg
      541 caagctggcc ctggtgggcg cggtgctcgg gtttctggtc acgttgccgt tgtacacggg
      601 cttcaatccg aacacggcca acatgcagtt cgtcgagaac gtgccctgga tcacgtcgct
      661 ggccatcaac taccatctgg gtgtcgacgg catctcgctg tggttcgtgc tgctcacctc
      721 gttcatcacc gtcatcgtgg tgctggcggg gtgggaatcg gtcacgcacc gcgtggcgca
      781 gtacatggcg gcgttcctga tcctgtccgg cttcatgatc ggggtgttcg ccgcgctcga
      841 cggcatgctg ttctacatct tcttcgaagc cacgctcatc ccgatgtaca tcatcatcgg
      901 tgcctggggc ggcccgaacc gggtgtacgc ggccttcaag ttcttcctgt acaccctgtt
      961 gggttcgctg ctcaccctga tcgccttcat ctatctgtgg cacgcgtcgg gtggctcgtt
     1021 cgacatcctc acgtggcaga acacgcggct tggctacacc gcgcagatcc tcatcttcgt
     1081 ggccctgatg gcctcgttcg cggtgaaagt gccgatgtgg ccggtgcaca cgtggttgcc
     1141 ggatgcgcac gtcgaggcgc ccacgggcgg ttcggtggtg ctggcggcca tcatgctgaa
     1201 gctgggcgcg tacggttacc tgcgtttctc gctgcctatc gcacccgacg cctcgcacag
     1261 cctggccggg ttgatcatca cgctatcgct ggtggcggtc atctacattg ggctcgttgc
     1321 catcgtccag gacgacatga agaaactggt ggcgtattcg tccgtggccc acatgggctt
     1381 cgtcaccttg ggtttcttca tcttcaacac ggcgggcgtc gaaggcgcca tcatgcagat
     1441 gatctcgcac ggcttcgtgt cgggtgccat gttcctgtgc atcggcgtca tgtacgaccg
     1501 actgcacacg cggcgcatcg ccgactatgg tggcgtcatc aacatgatgc cgcgcttcgt
     1561 cgctttcttc gtgctgttct cgatggccaa ctgcggcctg ccgggcacca gcggattcgt
     1621 gggtgagttc accgtcatca tgggcgcggt tcagcacaac ttctggatcg gcctgctggc
     1681 ggccacggcc ctgatctggg gcgcggggta ttcgctatgg atgctcaagc gcgtggccta
     1741 tggcgccgtc ggcaacaaca aggtcggcac catgaaagac ctgaacaagc gcgaattcct
     1801 catcatgggg atcatggcga tcgcggtgct gtacatgggg atctatccca agccgttctc
     1861 cgacgtcatg cactcgtcgg tacaggcctt gctgcaccat gtctccattt cgaaactgta
     1921 agcctggatc attatgcaaa gctcttttca catagccctt gcgacccccg agattctgct
     1981 gctgatcctg gcgacgctgg tgctgctggt cgacggcttc gggaagtccg tacagcggcg
     2041 caccaccttc gcgctgaccc tggtgtcgtt gctcatcctc acgctggtgt cgttgtggca
     2101 atggagcgag ggcgtgcatg gcagcgcctt cggcggcctg tatgtcgtgg acccgctctc
     2161 gcatctgctg aagatcgcca cctatcttgc cgtggcgctt atcctggtct acggacgcga
     2221 ctacgccgag gaacacgaga tcatgctgac cgggggcgag ttctattcgc tcacgctcat
     2281 gacactgctg ggccagatgg tgatgatctc ggccggcaac atgatcacgg tgtacctggg
     2341 aatcgagttg atgtcgttcc cgctgtatgc gctcatcgcc ctgcgccgcg acaacgtggc
     2401 ggccaccgag gcggccatga agtacttcgt gctgggcgcg ctgggcaccg gcatgctgct
     2461 ctatggcatg tccatgatct acggcgccac cggccagctg gggttgcagc agatcgccca
     2521 cgtcatcatc agtggtggcg cgcatcgcct gcccgtggtg ttcggcacgg tgttcgtcat
     2581 ttcggccttc gcgttcaagc tgggcaacgc cccgttccac atgtgggtgc ccgacgtcta
     2641 ccagggttca cccacctccg tcaccctggt cctgagcacc acgccctatc tggcggcctt
     2701 cgcggccatg gtgcggatgg tcatgctggg cctgcatggc gtggcgctcg actggcaacc
     2761 catgttgctg atcctggcgg tgctgtcgct ggcggtcggg aacatcgtcg ccattgcgca
     2821 gaccaacttc aagcgcatgc tggcctactc catgatcgcg cacatgggtt atgtgttcct
     2881 ggggctgttg tcgggcgtgg tggcaaaccg tcccgcggcg ctcgaacaag cctacggcgc
     2941 ctcgctgttc tacctgctga tctacgtgtt ctcgacctcg ctgaccttcg gcctgatcgt
     3001 gatcatgacc cgcaagggga tagaatgtga cgagatctcc gatctgaaag gcctgaatcg
     3061 tcgcaatccg gtgctggccg gcgcgctgct catggccatg ttctcgctgg cgggcattcc
     3121 gccgctggcc gggttctatg ccaaactgag cgtgctgcag gtgttggtgg gcgcggatca
     3181 tgtgacgatc gcggtggtgg cggtcatgct gtcgctgatc ggcgcgttct attatctgcg
     3241 tgtcgtcaag gtggcgtatt tcgaagaccc cgagggcgag gtcatcaccg gctccgccgg
     3301 cacggtcgcc aacgggctgc tgtcgctcaa cggcctgttg ctgctggggc tgggggtgtt
     3361 gcccggcggc ctgatggcct tgtgcatcaa ggccatcggc agctcgctgg ggctataggg
     3421 cgagggctga aagcctcttc cgccggtcgg ggtattcgca ccgtgggccg gccttgcggc
     3481 acaatggaac attaacttcg cgaagagagg catggaacag tccaccgcag tttggttcgt
     3541 cattgtgctg gccatcgtca ccgccaacct cccgttcctg atcgaacggc cgctgctcgc
     3601 gctgccatgg gcccgggagg gtgacgcgcg gccgtccggc gcgcgctggc tggaatcgct
     3661 ggtgttcttc ctgctgctgg tggcgctggg ctatttcatc cgcccgctca tcggcacggc
     3721 gactttctcc ggcgccgatc ccctgtcggt gctgctgttc ctgggcaaac tggcaggggg
     3781 gctgatcgcg gcggtgttgc tgctgtcgta caccggctgg cgcctgcgcg gcatggccgt
     3841 gcgcaagccg tttttcgagc ggctgctgga ggtcctggtt ttttatgcgc tggtgggcat
     3901 gctgggtttc ggattcgaga tgaacatcgg taacccgttc acgcagggct ggcagttcta
     3961 tgccgtgtcg ctgagcctgt tcctggtgct ggcttatccg ggatttgtct atcgctacct
     4021 gatgcgccat cacgcgcccg cccggaaagg cccggcggca aaacccgcgc gcccgtcggc
     4081 gtcaagcacg tcttcgcacg ccgcatcgca cggcggctga gcccgactcc tccacgcatg
     4141 ccatcaccgc aagctgtccg ccggggcctg gggtggggcc ggtgtaaggc gcccgcccgg
     4201 acgcggcaaa tccggatgaa gcacgccgtg ccggaaccgc cccaggcccc ggtggacagc
     4261 gtcttcagat accaacaaac aacgccgcaa aaccccagaa aacccctgaa gaataccctc
     4321 ggctctcaac ccccaagtca acacctgaac cagaaatacg ttagtagttg atcatataac
     4381 tactataatt ggactgataa tcagcactta cccgcccgag cggcgccggc tggtcttgag
     4441 gccggatcgt tcagtgcaat ttccggaaaa ccgtccatgc attcgatctc cagcatcgcc
     4501 cccaaaaatc cttccccaca agaatgcagc gaaccggcgc tgatgccgcg cacgcccaat
     4561 ctggccgacc ccgacgtcaa ccgcctgcgc gccacgctgc gtgaatactt cacggccacg
     4621 ttcgaccgtt atgaatccct cttcgagtgc ctggctaatg acgaggcgtt tgtcgtcaaa
     4681 tctatttcac tacgccatcc gctgattttc tacttcggcc ataccgccac gttcttcgtc
     4741 aacaagctgc tgctggcgcg tctcatcgac cagcgcatcg atgcccggct ggagtcgatc
     4801 ttcgccgtgg gcgtggatga aatgagctgg gacgatctgg acgacgcgaa ttacgagtgg
     4861 ccgtcggtgg acgaggtgcg ccgctaccgc gacaaagtgc gcgcgctggt cacgcggctc
     4921 atcgacaaga cgccgttgca gtcacccata ggctgggaca gcccatggtg ggccatcgtc
     4981 atgggtatcg agcacgagcg catccacttg gaaacctcgt cggtgttgat ccgccagcac
     5041 gccttgcaat atgtgcggcc ctccgagaac tgggtggcac tgaacgccga ggccggctcg
     5101 ccgcctgaca atgacctggt gcgcgtgcat ggcggcatgg tgcgcgtggg caaggcgctt
     5161 tcttccgcgc agtacggctg ggacaacgaa tacggtcgcc atgaagagta cgtcaacgat
     5221 tttcaggcca gccgttatct ggtcagcaac gccgagttcc tgctgttcgt cgaggcgggt
     5281 ggctatcaaa atcccgacta ttgggaagaa gaaggccgca agtggctggc gttcagccag
     5341 gtcacgcatc ccacattctg ggtgccgcac ggcgaggcct ggcgcctgcg cctcatgacc
     5401 gaagaagcgc ccatgccgtg ggactggccg gtggagacca attaccacga ggccaaggcg
     5461 ttctgtaatt ggaagaaggc tgaaaccggc caatcagtgc gcctgcccac cgaagacgaa
     5521 tggtatcgcc tgtacgacgt ggcgggtctg agcgacccgg gcgagtcgcg cgtggcggcc
     5581 aacctgcaac ttgatcattg ggcttcgtcg tgtccggtcg accgcttccg ccaaggccag
     5641 ttttatgatg tggtgggcaa cgtctggcaa tggaccgaga cccccacgta cccgttcgat
     5701 ggcttcgaca cgcacccctg gtacgatgac ttcaccacgc ccactttcga tggacgccac
     5761 aacctgatca agggcggatc ctggatttcc tgtggcaacg aagccgtgcg tgcctcacgc
     5821 tacgccttcc gtcgccactt cttccagcat gcgggcttcc gctatgtggt gtccgacgag
     5881 acgcctgtga acctcagtcc gtattacgaa accgacaaac tcatgtccga gtacgcggaa
     5941 ttccactacg gcgccgaata cttcggcgtg ccgaatttct cgcgcagcct ggtggaactg
     6001 gccaggccgt tcctggaagg ccggccgcgt gtcaacgcct tggatctggg ttgtgcttcg
     6061 gggcgagcct cgtttgaact ggcgcgacac ttcgaccacg tgaccggtgt cgatttctcg
     6121 gcccggttca ttgagcaagg cgtgttgctg gcggggcagg gcgtgctgcg ctacacgctg
     6181 gctgatgaag gcgaactggt gtcgtattgc gccagcagcc tggcggagct gggcctagac
     6241 gacgtgcgca acaaggtggc gttcttccaa ggtgatgcgt gcaacctgaa gccgcagtac
     6301 gccggctacg acttcgtgct ggccgcgaat ctcatcgacc gcctttacag tccggccaag
     6361 ttcctggatg ccatccatga gcgcatcgtg cccggcggtg tgctcatgat cgcctcgccc
     6421 tacacttggc tggtcgagca tacgccgcgc gaagcctgga taggcggatt caagaaagat
     6481 ggcgaaagct acaccacgct ggatggtctg aaggcgcatc tgggcgcgca tttccgcctg
     6541 ctgcacgggc cggccgaagt gccgttcgtc attcgtgaaa ccaagcggaa attccagcac
     6601 acgctgtcgg aagtgacatt gtgggagcgg gtgaggtagg gaagttgttt gcagaattgg
     6661 gcatgatggg tatttgaagg cgctgtccat cgaggcctgg ggtggatccg gcgcacgcct
     6721 tacaccggcc ccaccccagg cctcgatgga cagctcgtgg tgacggcatg ccgggggagg
     6781 gggccaacca gcccgcctca ttcgcgtata gatcggtgtc gccaatgtcc atcaccaatg
     6841 cgagtaagat gaagacgtgg cgtttggcgc tggggtgagc ccgggagggc gccgatatgg
     6901 gaaaggcagt gcgatggttc ttcttgtgcg gcatgtcatg gctgcttggc ggagctgtat
     6961 cgccggcttt cgggcaggcg cgtgccgctg tggcttccca cgaagtcgtc gtgctcaagg
     7021 tggacggcat catcagcccg gcctcgtctg acttcatcct cgccgggatc gacagggcga
     7081 cgcatgacaa cgctcaactg gtcgtcatcg aactcgacac gccgggcgga ttggattcct
     7141 cgatgcggac catcgtccag cacatcctgg cgtcacccgt accggtcgcc accttcgtca
     7201 gtcctgcggg cgctcgtgcc gccagcgcgg gaacgttcat tctgtacgcc agccatatcg
     7261 cggccatgac gccggccagc aacctgggcg cggcgtcgcc agtaacgatt ggcctgggtg
     7321 gaaacgatga agcgccggcg aagccgtcgg catcgtcacg gccctccagt tccccggctg
     7381 ggcaacgctc tgactcgtcg gtagggcaac aagcatcaac ccagccacag ctcagcgagg
     7441 gcgacaccat gaaaaagaag atcatgaacg acgccgttgc ctacatccgc agtcttgcgc
     7501 aactgcgcgg ccgcaacgcc gatttcgcgg aaaaggcggt gcgcgacgcg gccagcctgt
     7561 ccgcgcccga ggcgctgaag gacggggtga tcgacctgat cgccgacaac ctggatcagt
     7621 tgctgaccaa gcttgatggc cggcaggtca aactggggtc gggtcgcgtg gtgacactgg
     7681 cgacgagcca accggacatc gtcgtgttgt cgccggactg gcgcacccgg gtgctgggtt
     7741 tcatcgccga tccgcagatc gccttgattc tgctgatggc gggtgtgttc gggctgttct
     7801 acgaagccat gaatccagga ctggctgtgc ctggtgtggc gggcctgatc agcctgttgc
     7861 ttggcctgta cgcgttccag atgctgccag tgaattgggt gggtgtcggg ctcatcgcgc
     7921 tgggcacggc gttgatgatc gccgaagcgt tcatgccgac gtttggcgtg gtcggcgccg
     7981 ggggcgtggt ggcgttcgtg ctgggcggca tcttcctgac cgatacaacg ataccgggct
     8041 atggcttgtc ggtgccgttc ctgatcggca tcgcggtggc ctgtgccatc ctgttcttca
     8101 tcgtgggcag tgtggcggcg cgggcgcacc ggcgtcgcgt ggtcaccggc gccgaagaga
     8161 tgcggggcct gcgcggagtc gtcacgtcgg tcaacggcgc ggtcgtctac gccgaggttc
     8221 gcggcgagcg ctggaaggtg cgtagcccgc aggcgttggc gcccggcgac ctggtgcgcg
     8281 tcgtgagcct gaatggactg accttgctgg tcgaacccgc cgatgatgca cattcaatga
     8341 aagacaaagg ggaacactgt gtcctatagc tacgatatcg ttgtgatcgc cgtcgtcatc
     8401 atcctggccc tggtgttgat caacgccgtc cggatattgc gtgagtatca gcgcgccgtt
     8461 gtcttcacac tggggcggtt ctcgggggtc aaggggccgg ggctgatctt cgtgatacct
     8521 gtggtgcagc agatcgtgcg cgtcgacctg cgcgtcgtga ccatggacgt gcccagccag
     8581 gacgtcattt cgcgcgacaa cgtgtcggtg aaggtcgatg ccgtcctgta tttccgcgtc
     8641 atggcgccgg acaaggccat catccaggtg gagcgctacc tggaggccac cagccaactc
     8701 gcgcagacca cgctgcgcgc ggtgctgggc aagcatgacc tggacgacat gctgtccgag
     8761 cgcgagaaac tgaacttgga tgtgcaggaa atcctggatg cgcaaacgga ctcatggggc
     8821 atcaaggtca ccaacgtcga gatcaaacgc atcgacctga atgagaacat gatccgcgcc
     8881 atcgcccggc aggccgaggc tgaacgcgaa cggcgcgcga aggtcatcca cgccgaaggc
     8941 gagaaacagg ccgcccaggc gctcatggag gccgccgaaa ccctggccaa gcagccctcg
     9001 gcgatgcagt tgcggtactt gcagacgctg acccaggtgg cgggggacaa gagttcgacg
     9061 ctggtgtttc cgataccggt ggatttgctg tcagccctca tgaacaacaa ggtgggcggg
     9121 cccggggcgt cgttgttgca gtatccaccg gaggggccgg cgggggcgcc ggacaagcct
     9181 gggtcgtgat catcgcgtcg gcagccttcc gcgaagtcat ctaattcgtt ggcgggtggg
     9241 cgcggcgact gccatcagag cgctcccgaa gagccgccgg acttgcagga acgccacgtt
     9301 tcgccctggt gccatcagcg ggaaccagaa cattatgaag agctttcgct taacattgcg
     9361 gctagtcttt atcgcggtcg cgtgtgcgtc g
//
LOCUS       DASTNK010000073         8543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_792851, whole genome shotgun sequence.
ACCESSION   DASTNK010000073 DASTNK010000000
VERSION     DASTNK010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8543
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792851"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /gene="rsmA"
                     /locus_tag="VFR20_05630"
     CDS             complement(<1..561)
                     /gene="rsmA"
                     /locus_tag="VFR20_05630"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU0230031.1"
                     /translation="MASHQARKRFGQHFLTDEGVVEAIVRAIAPSADDAMVEIGPGLS
                     ALTEPLLRAVTRLTVVELDRDLAARLRTRHNESRLRVVEGDALNVDFSAFGPRLRIVG
                     NLPYNISSPLLFHLLNCADQVMDQHFMLQREVVDRMTAQPATSDYGRLSVMLQARYAM
                     EKLFDVAPESFDPPPRVVSAVVRMVPL"
     gene            complement(569..2083)
                     /locus_tag="VFR20_05635"
     CDS             complement(569..2083)
                     /locus_tag="VFR20_05635"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0230032.1"
                     /translation="MMLRPCSYRRLTPALAAAVFMLTCGSAIAARHVTHTTPPAKSAS
                     SSPQFVDGIAAVVNKDVITLGQLAERTRQAQEQLAKQKITAPDLQTLRRQVLNRMITD
                     QLEQQEAKRLGIVVTDEQLQAAIKSVAERNKIPVDRLRTEVAKTGVSWDEYKRQVHQE
                     VLQDELRRRAVDSTIRVSDADVDAYLKTHEGGASLFGGTTPAQTPALARPTAAQSGPV
                     MLGLAQILVAVPDNASSARVAELRHKAEDILAKLRAGADFSSLAASSSDGPQSMQGGN
                     LGVKPVDGWPDLFVSATSKLKVGQVTGILQSGNGFHILKVLSRSGASQAPASPPPSEA
                     AAPGAPQEAPVDNGPEVVTQTHARHILIKVTQVMSDAQAKQRLLELRQQIENGADFAK
                     LAERYSQDASAPQGGDLGWLNPGETVPAFEKAMNALKVGQVSEPVKTRFGWHLIQVLG
                     RRTKNVTEEVQRNQARRILFERRAGPAFEEWLSQLRGRAYIDNRLDPAENQSGS"
     gene            complement(2080..4491)
                     /locus_tag="VFR20_05640"
     CDS             complement(2080..4491)
                     /locus_tag="VFR20_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEU0230033.1"
                     /translation="MRWVWWAIFFAVGGVPVARADTAGPPIGAGLAASPATPAPTLDL
                     SVAPNLQLHKHLNEDQMSVFLSADKIQSEPGGKVILTGAAEVRRIDSVVKGDHIDYQR
                     DTGEVHVRGNGLIMRDASIVTGPEMDYNVNSQSGKVEYPSFWLGANGSFGKAKQADIL
                     DRHDMRLHDVVYAGCGCSDPPWYIESPTVDLHFQENEGVARNGVLYFKGVPLLASPYL
                     SFPIRKERKSGFLIPTYGMSSNTGVELSLPYYLNLAPNYDATLTPRLMSKRGIQLGAE
                     FRYLGSSYSGSLSGTYLPKDNITGDPRWMYIVQHNQDFGHGLGLSLDMRRVSDDNYFR
                     DFSTFGVLNDTTTTYLPSVATLTSAGHKYWYATVQSYTYQTLQDSTTNYLYPPFDKLP
                     ELHFNASRYGWGGFDVESDNYVTRFRSPVYTGGIWNASYGSVYGLHTRPDGTRFMSYD
                     TISYPITRAGWYVTPKVGLHMSQYNTNWYNLPGYQGYPDTQSRVLPIVSVDSGMTFER
                     NTTLFGNDAIQTLEPRVYYLWVPYRDQSQIPVYDTALATFNFTQAFSDNIFSGWDRIA
                     NANQVTLGLTSRWLDADTGFERLSVSAAQRIYFTNQRVTLPGQSDFARTNGQSDYLFG
                     VNAALTDKLNVQFNAQYSPQTHERNQMSASFRWQPKRLATLSVGYRYERDPAQLLNPA
                     VVNSPGYVDNSRNQVSVSGQWPLTQKWYMMGRYDYSFQENRNTQTIVGLEYKGDCCWT
                     ARAVFERYAISSQDVNNAMFFQLELTGLGSLGTDPMSLLRDRIIGYEPVTPPTPEKTT
                     FERYE"
     gene            4809..5861
                     /locus_tag="VFR20_05645"
     CDS             4809..5861
                     /locus_tag="VFR20_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU0230034.1"
                     /translation="MPPVTPPESDSRMAALGAWLDSLAPKHHLRVDTLCAASSDASFR
                     RYFRLQAGERTLIVMDAPPPHEDCRPFVHVAGLLSDTGINVPAILAQDTRQGFLLLSD
                     LGTQTYYQAIQAGMDDATLQQAYRDALGGLVRMQQATTTGLPIYDEARLLEELALFPE
                     WYAARHCKVELDAGERNVLREAFRLLARDNAAQPTVFVHRDFHSPNLMLCDGDEYGPN
                     PGVIDFQDALRGPLTYDLASLVMDARTTWDEAQQLDWAIRYWQAARSAGLPVPADFSD
                     LHRAYEWMGLQRNLRILGVFARLSHRDGKHHYLDHMPRVSAYVRQVAGRYAVFGPLLR
                     LLDRLDNRQVGVGYTF"
     gene            5861..6580
                     /locus_tag="VFR20_05650"
     CDS             5861..6580
                     /locus_tag="VFR20_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU0230035.1"
                     /translation="MRAMILAAGRGERMRPLTDTVPKPLLRAGGKPLIVWHVEHLVGA
                     GVTELVINHAWLGHRIVDTLGDGTRFGACIRYSAEDPALETAGGIAHAMPLLGSEPFL
                     VVNGDIWCDWNPAAADRISRELGSAHAWLLLADNPPHHPDGDFHLDVDGHVSEPSSGA
                     DSLTFSGIGVYRPELFERIDPNQPAPLSPLLRQAMQEGRIKGARHAGFWMDIGTPQRL
                     AALDQRLAGQDTRATTHPPAR"
     gene            6768..7475
                     /locus_tag="VFR20_05655"
     CDS             6768..7475
                     /locus_tag="VFR20_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HEU0230036.1"
                     /translation="MPMFGSSKQNVFRPTAYGSTRRPRRIPRWLVLILTGIVLGAGGL
                     LFLQKSYGPTRLTVEQSEQLHFDLNSANLDKQRLQSQLNQSNHDLTAARSQLTSQTTQ
                     LKQAQDEIAKLNKDINLFADAMPEDPRGTSPGIRAASFSVDNGSLNYQILVMQDKNKT
                     TTFQGSMELLAEGRYTNGKRATISLDPVAFSVQRYTHLVGTADLPAGFTPYVITIHVT
                     NQATKKISATRTIRVSR"
     gene            7830..>8543
                     /locus_tag="VFR20_05660"
     CDS             7830..>8543
                     /locus_tag="VFR20_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU0230037.1"
                     /translation="MTATSHISATAQARARGTGLRGYLLSLSRTRKRVLQLVVDTLLI
                     WLCLWLAFYLRLGSFDAASPFFHGWLFIAAVLITLPIFVRFGLYRAVVHCMGYQALTA
                     LTAAITTSAILLICTIYLFASPPEIIPRSIIFIYWMLCLIFIGGLRVAMRQYFGHTLT
                     LNLDIRSLLHVSGKHQGSQTRVAIFGAGAAGNQLLAALRQSGRQYVVAFVDDDPLIAG
                     RLVAGVLVYASSDLAHFMKE"
BASE COUNT         1655 a         2777 c         2498 g         1613 t
ORIGIN      
        1 caacggcacc atgcgcacca cggccgacac gacccgtggc ggcggatcga acgactcggg
       61 cgccacgtcg aacagcttct ccatggcata acgcgcctgg agcatgaccg acagccggcc
      121 gtaatccgac gtggcgggct gtgccgtcat gcgatccacc acctcgcgct gcagcatgaa
      181 atgctggtcc atgacctgat cggcgcaatt cagcaggtga aacaacaacg ggctcgatat
      241 gttgtacggc agattgccca cgatgcgcag acgcggtcca aatgccgaaa agtcgacatt
      301 caacgcgtcg ccctccacca cacgcagcct cgattcgttg tggcgggtgc gcaggcgcgc
      361 cgccagatcc cggtcgagct cgaccaccgt caggcgcgtc acggcacgca gcaagggttc
      421 tgtcagcgcc gagagcccgg ggccgatctc taccatggca tcgtcggccg acggggcgat
      481 ggcacgcaca atggcctcca ccaccccctc atcggtcagg aaatgctggc cgaagcgttt
      541 gcgtgcctga tgcgaagcca ttgtgcgctc aactccccga ctgattctct gccggatcca
      601 ggcgattgtc gatgtaagcg cgaccacgca actggctgag ccactcttca aatgcgggcc
      661 ccgcgcggcg ctcgaacaga atgcggcgcg cctgattgcg ttgaacttct tctgtcacgt
      721 tcttcgtccg acgccccagg acttgaatca gatgccagcc aaaacgcgtc ttcaccggct
      781 cgctgacctg acccaccttg agcgcattca tggctttctc gaacgcaggc accgtttcac
      841 cggggttgag ccagccgaga tcgccgcctt gcggggccga cgcatcctgc gagtatcgct
      901 ccgcgagctt ggcgaaatcc gcaccgtttt caatctgctg gcgcagctcg agcagacgct
      961 gctttgcctg cgcatcggac atcacctggg tgaccttgat gagaatgtgc cgcgcatggg
     1021 tctgcgtcac cacctccggg ccattgtcga ccggcgcttc ctgtggagcc cccggcgccg
     1081 cggcttccga tggcggcggg gaagcgggag cctggctggc tccgctacgc gaaagtacct
     1141 tcaggatgtg gaacccattg ccactctgca gaatgccggt gacctggccc actttgagct
     1201 tggacgtcgc ggaaacaaac aaatccggcc acccgtcaac cggtttgacg ccaaggttgc
     1261 ccccctgcat cgactgcggt ccatcggagg aagacgccgc cagactggag aaatcagcgc
     1321 ccgcgcgcag tttcgccaga atgtcctcgg ccttatggcg caactccgcc actcgggctg
     1381 atgatgcgtt atccggcacg gctaccagaa tctgcgctaa accgagcatg accggaccgg
     1441 attgcgccgc agtgggtcgt gccaatgccg gcgtttgcgc gggcgtcgtc cctccgaaca
     1501 ggctggcccc gccttcgtgt gtcttcagat aggcatcgac atcggcgtcc gacaccctga
     1561 tggtgctgtc caccgcacgg cgacgaagct cgtcctgcag cacttcctgg tgcacttggc
     1621 gcttgtattc atcccacgaa acaccggtct tcgcgacctc ggtgcgcagg cgatcgaccg
     1681 gaatcttgtt gcgctccgcc acggatttaa tggcggcctg aagctgctcg tcggtgacga
     1741 cgatacccaa ccgcttggct tcctgctgct caagttgatc ggtgatcatg cggttgagca
     1801 cctgtcggcg caacgtctgt aaatccggcg ccgtgatctt ctgtttcgcg agttgctctt
     1861 gtgcctggcg cgtgcgctcg gcgagctgac caagcgtgat cacgtctttg ttcaccacgg
     1921 cggcgatgcc gtcgacgaac tggggagagg acgacgcgct cttggccggc ggcgtggtgt
     1981 gcgtgacgtg ccgggccgcg atggctgagc cacacgtcag catgaagacc gcggcggcca
     2041 gcgccggcgt gagccggcgg taggagcaag gtcggagcat cattcgtacc tctcgaaggt
     2101 ggttttttcg ggtgtgggtg gggtcacggg ttcgtagccg atgatgcggt cgcgcagcag
     2161 gctcatggga tcggtgccca acgagccaag gcctgtgagc tcgagctgga agaacatggc
     2221 gttgttgacg tcctgactgg agatggcata acgctcgaac accgcgcggg cggtccagca
     2281 gcaatccccc ttgtactcca gtccgacgat ggtctgggtg ttgcgatttt cctggaacga
     2341 ataatcgtag cgccccatca tgtaccactt ttgggtgagc ggccattggc ccgagacgga
     2401 gacttgatta cggctattgt cgacgtatcc agggctgttg acgacggcgg gattgagcaa
     2461 ttgcgccgga tcccgttcat agcggtagcc cacggaaagt gtcgccagac gcttgggctg
     2521 ccagcgaaaa gacgccgaca tctgattgcg ctcgtgcgtc tgggggctgt attgggcgtt
     2581 gaactgaacg ttgagcttgt cggtcagcgc ggcattgaca ccgaacagat aatcggactg
     2641 cccgttggtg cgggcaaaat cggattggcc gggaagcgtg acgcgctgat tggtgaaata
     2701 tatgcgctgc gccgccgaca cggaaaggcg ttcgaatccc gtatcggcat ccagccagcg
     2761 gctggtcaac ccgagcgtga cctgattggc attggcgatg cggtcccatc cgctgaagat
     2821 attgtcgctg aacgcctggg tgaaattgaa cgtggccagc gccgtgtcat agaccgggat
     2881 ctgggactgg tcgcgatagg gcacccacag ataatagaca cggggctcca gcgtctggat
     2941 ggcatcgttg ccgaacaaag tggtgttgcg ctcgaaggtc atgcccgaat cgaccgacac
     3001 gatcggcagc acccggctct gcgtgtcggg gtagccttga taaccaggca agttgtacca
     3061 gttggtgttg tactgggaca tatgcagccc gaccttgggc gtgacatacc agcccgcgcg
     3121 ggtgatgggg tatgaaatcg tgtcgtacga catgaaccgc gtcccgtcgg gccgggtatg
     3181 gaggccatag acgctgccat acgacgcgtt ccatatcccc ccggtgtaaa ccggagaccg
     3241 gaaccgcgtg acatagttgt cgctttccac atcgaaccca ccccaaccgt accgggaagc
     3301 gttgaaatgc agctccggca gtttgtcgaa cggcgggtac agataattgg tggtggaatc
     3361 ctgcaacgtc tggtacgtgt acgactgcac cgtggcgtac cagtatttgt ggccggcgga
     3421 cgtcagggtg gcgacactgg gcagataggt cgtggtggtg tcattgagca caccaaacgt
     3481 cgagaaatcg cgaaaataat tgtcgtcgga cacacgccgc atatccaggg acaggcccag
     3541 gccatggccg aaatcctggt tgtgctggac gatgtacatc cagcgcggat cgcccgtgat
     3601 gttgtctttg ggcagatacg tcccgctgag cgacccgcta tagctggacc ccagataccg
     3661 gaattcggca ccaagctgaa tgccgcgttt actcatcaga cgcggcgtca gtgtcgcatc
     3721 gtaattgggc gccaggttca ggtaatacgg cagcgacagc tccaccccgg tattgctgga
     3781 catgccatag gtggggatca gaaaacccga cttgcgctct ttgcgtatcg ggaaagacag
     3841 atacggggac gccagaaggg gcaccccctt gaaatacagc accccgttgc gcgcgacgcc
     3901 ttcgttttcc tggaaatgca gatccaccgt cggcgattcg atgtaccaag gtggatcgga
     3961 gcacccacat ccggcataca cgacatcatg cagacgcatg tcgtggcggt cgagtatgtc
     4021 ggcctgcttg gccttgccga agctgccatt ggcacccaac cagaaactgg ggtactcgac
     4081 cttcccggat tggctgttca cgttgtagtc catctcggga cccgtgacga tggacgcatc
     4141 gcgcatgatg agcccattcc cccggacatg cacctcgccc gtgtcgcgct gatagtcgat
     4201 atgatcgccc ttgacgactg agtctatgcg ccgcacctcc gcggcaccgg tcaggatgac
     4261 tttgccaccg ggttccgact ggatcttgtc ggcggacagg aacaccgaca tctggtcttc
     4321 gttcagatgc ttgtgcaatt gcaggttggg ggcgacagac agatcgaggg tgggcgcggg
     4381 cgtggccggc gatgcggcca gcccggcgcc gatcggtggc ccggccgtat cggcccgcgc
     4441 caccggcacg ccgccgaccg caaagaagat tgcccaccag acccaacgca cgagtaaccc
     4501 gtctcctgaa aaccgccaaa tagtcagacc tcgaggcccg ccctcagggg cgaaccaccg
     4561 atcgcggccc gctctggccg cgatcgtgat gatgaaaacg cctggcccgt catggcgctc
     4621 cgccctggca ctgggcaccg cccagcatga acaggccctg tattatagga gaagcaagcg
     4681 tttgctccgg gaaactcaaa gtaaaatcac gtccatcccg gcccccgggg ctgccgcggc
     4741 cgtgacagcc ccgcacaggc cgcaactctc gccgaaacgc tttgcaacaa ccccaaccgc
     4801 aggaatccat gcctcccgtc accccacccg aatccgactc aagaatggcg gcgctgggcg
     4861 cctggttgga ttcactggcc ccgaagcatc acctcagagt cgacaccttg tgtgccgcct
     4921 cgagcgacgc cagcttccgc cgctacttcc gcttgcaggc aggggaacgc accctcattg
     4981 tcatggatgc cccgcccccg cacgaggact gccggccgtt cgttcatgtg gccgggcttc
     5041 tgtccgacac gggaatcaat gtgccggcca tcttagcgca agatactcgg cagggctttc
     5101 tacttttgtc ggacctcggg actcaaacct actaccaagc cattcaggcc ggcatggacg
     5161 acgcgacgct ccaacaagca taccgggacg ccctgggcgg cttggtgcgg atgcagcagg
     5221 ccaccaccac cggcctgccc atctacgatg aggcgcgctt gctggaagaa ctggcattgt
     5281 tccccgaatg gtatgccgcc aggcattgca aggtggaact cgatgcgggc gagcgcaacg
     5341 tgctgcggga ggcattccgt ctgctcgccc gggacaacgc cgcacaaccc accgtgttcg
     5401 tgcatcgcga cttccactcc cccaacctga tgctgtgcga cggagacgaa tacggtccga
     5461 atcccggtgt cattgatttc caggatgcgc tgcggggccc actgacatac gacctggctt
     5521 cgctggtcat ggacgcgcgc accacatggg acgaggccca gcaactcgac tgggccatcc
     5581 gctactggca ggctgcacgc tccgccggcc tgccggtccc tgccgatttc tccgacctgc
     5641 accgcgcgta cgagtggatg gggctgcaac gcaatctgcg catccttggc gtgttcgccc
     5701 gcctctccca ccgagatggc aagcaccact acctcgacca catgccgcgg gtcagcgcct
     5761 atgtccgcca agtcgcgggg cgctatgccg tgttcgggcc gctgctgcgc ctgctcgatc
     5821 gcctggacaa ccgccaggtc ggcgtcggat acaccttctg atgcgcgcca tgatcctggc
     5881 cgcgggccgc ggcgaacgca tgcgccctct gaccgacacg gtgcccaaac ccttgctgcg
     5941 ggccggcggg aaacccctga tcgtttggca tgttgaacac ttggtcggcg cgggtgtcac
     6001 tgaactggtg atcaaccacg cctggctggg ccaccgcatc gtcgacaccc tgggcgacgg
     6061 cacccgtttt ggcgcgtgca ttcgctattc cgccgaggat cccgccctgg aaacggccgg
     6121 tggcatcgcc cacgccatgc cgctactggg aagcgaaccg ttcctggtgg tcaatggcga
     6181 catctggtgt gactggaacc ccgccgcggc cgaccgcatc agccgcgaac tggggtcggc
     6241 ccacgcatgg ctgctactcg ccgacaaccc accccatcat cccgacggag actttcatct
     6301 ggatgtcgac ggccatgttt ccgagccgtc atcaggggcc gattcgctca cattctcggg
     6361 catcggtgtc taccgacccg aactgttcga acgaatcgat ccgaaccagc ccgcaccact
     6421 gtcgccactc ttgagacaag ccatgcaaga agggcggatc aaaggggcgc gacacgcggg
     6481 attctggatg gacatcggca cgccgcaacg cctggcggca ctcgatcaac gcctggcggg
     6541 acaagacacc cgcgccacca cacacccacc cgcgcgctag aaatccggtc tgacccacgt
     6601 tacacttact cctttgccca cgctggcgac atgtatcaaa aaccgccaaa gcgaaaccgt
     6661 ggccatcagg ccgtggtcag cgcgcgtttt ccagtctaag ctagcaggct tttcccaccc
     6721 ctcggccttt ccggccccgg cggttcgcaa tacagggacc accccccatg cccatgttcg
     6781 gctcatcaaa acagaacgta ttccgaccca cagcctatgg cagcacccgg cgcccccggc
     6841 gcattccccg ctggctggtg ctgatcctga ccggtattgt cctgggtgcg ggcggactgc
     6901 tcttccttca gaaaagctac ggccccacac gcctgaccgt cgaacaatcc gaacagttgc
     6961 acttcgacct gaacagcgcc aatctggaca agcagcgcct gcaatcccaa ttgaaccagt
     7021 cgaaccacga cctgaccgcc gcgcgcagcc agctcacgtc acagaccaca caactcaagc
     7081 aggcccagga cgaaatcgcg aagctcaaca aggacatcaa cttgttcgcg gacgccatgc
     7141 ccgaagatcc ccgcggcacg tcgcccggta ttcgcgccgc cagcttttcg gtcgacaatg
     7201 gctcgctgaa ctatcaaatc ctggtcatgc aggacaagaa caagaccacc actttccagg
     7261 gcagcatgga actgctggcc gaaggccgct acaccaacgg caagcgcgcc accatttccc
     7321 tggacccggt ggccttttcg gtgcagcgct atacccacct ggtcggcacg gccgatctac
     7381 cggcgggttt cacgccttac gtgatcacca tccacgtcac caatcaagca acgaagaaaa
     7441 tcagcgcaac gcgcaccata cgcgtgtcgc gctgattgtc cgatgtgcgg ggcactgcag
     7501 ccccgttctt cgtgagcaag gcgttgattg cgcctgacat atagaacact ggtcgtgcct
     7561 gacatgcggg atgctgattg ccccgcgagc acgaactccg acacgagcgc aagaactcgc
     7621 tgatccggag cattgcctcg ttcctcttcg cccaactacg ctattttccc caggccctga
     7681 ggagagtagc catgcccatg ccctaagccg aacgctgtct atctcaaaac gtgtctcgtc
     7741 tctaagattc atttcgttag atacgtttcc gaaaagtgac aagcggtcac taaataccgc
     7801 gacagcaatt ggaaaaggac aagatggcct tgaccgctac ttcgcatatc tcagcgacgg
     7861 cgcaagcccg cgcgcggggc acggggttgc gcggctatct gctgagcctg tcacgcacac
     7921 ggaaacgcgt gctgcaactc gtagtcgata cattgctcat ctggctctgt ctttggctgg
     7981 ccttttactt acgattaggt tccttcgatg cagcctcgcc attctttcac ggctggctct
     8041 tcatcgccgc tgtcctgatc acgctaccca tattcgtacg gttcgggctg taccgggcgg
     8101 tcgtgcattg catggggtat caggccctga ccgccttgac cgcggccatc acgacgtcag
     8161 ccatcctcct gatatgcacc atctaccttt tcgcctcgcc acccgaaatc attccacgct
     8221 cgatcatctt catatactgg atgctctgcc tgatcttcat cggcgggctg cgcgtggcta
     8281 tgcggcagta cttcggccac acgctcacgc tcaacctgga catccggtca cttctgcacg
     8341 tcagtggcaa acaccaaggc tctcagacac gggtcgccat cttcggtgcc ggcgcggcgg
     8401 gtaatcagtt gctggcggcc ctgcgccaaa gcgggcgcca atacgtcgtg gccttcgtcg
     8461 acgacgaccc gctgatcgcg ggccggctgg tcgccggcgt actcgtctat gcttccagcg
     8521 acctcgccca cttcatgaag gaa
//
LOCUS       DASTNK010000074         2261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1349693, whole genome shotgun sequence.
ACCESSION   DASTNK010000074 DASTNK010000000
VERSION     DASTNK010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349693"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..565
                     /locus_tag="VFR20_05665"
     CDS             2..565
                     /locus_tag="VFR20_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009464175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU0230038.1"
                     /translation="MALALAVAAVSASSAASAQTVDNWTNPFGQVWKNGTRELCWRDN
                     FWTPATGIAGCDGVPVAQAPAVKAPTATKIVLNADTFFDFDKSVIKPEGRRILDQVAT
                     KVQSMNLETLIATGYTDSIGTKAYNLKLSQRRADAVKKYLVSRGIDANHIYAEGKGMS
                     NPIASNKTREGRAHNRRVEIEIVGTNK"
     gene            654..1382
                     /gene="ubiG"
                     /locus_tag="VFR20_05670"
     CDS             654..1382
                     /gene="ubiG"
                     /locus_tag="VFR20_05670"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEU0230039.1"
                     /translation="MTDTPSSATTHNADPAELEKFGALAARWWDPESEFKPLHAINPL
                     RLGWIREHVGSLADKDIVDIGCGGGILAESMALEGARVTGIDLAEKSLTVARLHGLES
                     GVSVNYQAISAEQLAADRPAQFDIVTCMEMLEHVPDPVSVVRACATLAKPGAWVFFST
                     LNRNPKSFLFAILGAEYVLKLLPRGTHSYENFIKPSELAAAGRQSGLDVTTLAGMEYN
                     PITRHYALTRDTSVNYLVATRRPA"
     gene            1400..2062
                     /gene="gph"
                     /locus_tag="VFR20_05675"
     CDS             1400..2062
                     /gene="gph"
                     /locus_tag="VFR20_05675"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523177.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEU0230040.1"
                     /translation="MSKLILFDFDGTLADTAPDLAAAANKARARKGLPPLPYATLRPM
                     ASQGARGLLKAGLGMDPDHPDYEACRQQFLDDYEQDMTSGTSLFPGVPALLATLRDNG
                     YRWGIVTNKVEYLALPLVRHLGLDAECAITVGGDTTANRKPHPAPLLYAADKAGFAPD
                     QCLYIGDDERDIQAGKAAGMKTIAAAYGYCGHGGAEQWHADALAATPDEVWPAIRQLL
                     PD"
     gene            2123..>2261
                     /gene="ssrA"
                     /locus_tag="VFR20_05680"
     tmRNA           2123..>2261
                     /gene="ssrA"
                     /locus_tag="VFR20_05680"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          469 a          829 c          612 g          351 t
ORIGIN      
        1 aatcgcactt gcactcgctg tcgccgccgt ttcggcttcc agcgccgcct ctgcgcaaac
       61 cgtcgacaac tggaccaacc ccttcggcca agtctggaaa aacggcaccc gtgaactgtg
      121 ctggcgcgac aatttctgga cacccgccac cggcatcgcc ggctgcgatg gcgtaccggt
      181 tgctcaagct cccgccgtca aggcccccac ggccaccaag atcgtcctga atgccgacac
      241 cttcttcgac ttcgacaagt ccgtcatcaa gcccgaaggc cgtcgcatcc tcgaccaggt
      301 tgccacgaaa gtgcaatcca tgaacctgga aaccctgatc gccaccggct acacggactc
      361 catcggtacc aaggcctaca acctgaagct gtcgcaacgt cgcgccgacg cggtcaagaa
      421 gtacttggtc agccgcggca tcgatgccaa ccacatctac gcggaaggca agggcatgtc
      481 caaccccatc gcctcgaaca agacccgcga aggccgcgca cacaaccgtc gcgtcgagat
      541 cgaaatcgtc ggcacgaaca agtaacaccg ccgcctcgct gcagcaaacg tagcgctagc
      601 cgctacaata aaggctccgc tctgcggagc ctttattcat tccgctccac accatgaccg
      661 acactccatc ctccgccacc acccataacg ccgaccccgc cgaactggaa aaattcggcg
      721 cgttggccgc ccgttggtgg gatccggaaa gcgagttcaa gccgctgcac gccatcaacc
      781 ccctgcgctt gggctggata cgtgaacacg ttggttcact ggccgataaa gacattgtcg
      841 atatcggctg cggcggcggc atcctggccg aaagcatggc gctggaaggc gcccgcgtca
      901 ccggcatcga cctcgccgaa aagtctctca ccgtcgcacg cctgcatggc ctggaatccg
      961 gcgtgtccgt gaactaccag gcgatcagcg ccgaacaact cgccgccgac cgccccgcgc
     1021 agttcgatat cgtcacctgc atggagatgc tcgaacacgt tcccgatccg gtgtcggtgg
     1081 tgcgcgcctg cgccacgctc gccaaacccg gcgcatgggt gttcttctcc accctgaacc
     1141 gcaaccccaa atcgttcctg ttcgccatac tgggtgccga gtacgtgctc aagctgctgc
     1201 cacgcggcac gcactcgtac gaaaacttca tcaagcccag cgaactggcc gcggcgggcc
     1261 gccaaagcgg gctcgacgtc accacgctgg ccggcatgga atacaacccc atcacccggc
     1321 attacgcgct cacgcgcgac acctcggtga actacctggt cgcgacgcga cgccccgcct
     1381 gatcctcaca tacccgcgca tgtcaaaact gatcctgttc gacttcgacg gcacgctggc
     1441 cgacacggcg cccgacctgg ccgccgccgc caacaaggcc cgggcccgca aaggcctgcc
     1501 ccccctgccc tatgcaaccc tgcgccccat ggcatcgcag ggcgcgcgcg gcctgctcaa
     1561 agctgggctc ggcatggatc ccgaccaccc cgattacgaa gcgtgccggc aacaattcct
     1621 ggacgactac gagcaagaca tgacttccgg gacctccttg ttccccggcg tccccgcgtt
     1681 actggccacc ctcagggaca acggttaccg ctggggcatc gtgaccaaca aagtcgaata
     1741 cctggcgctg ccgctggtgc gccacctggg gctggacgcc gagtgcgcca tcacggtggg
     1801 cggcgacaca acggccaacc gcaagcccca ccccgcccca cttctctacg cggcggacaa
     1861 ggccggcttc gcgcccgacc aatgcctgta catcggcgac gacgagcgcg acatccaggc
     1921 cggcaaggcc gctggcatga agaccatcgc cgcggcctat ggctactgcg gtcacggcgg
     1981 cgctgaacaa tggcatgccg acgcgctggc cgccacgcca gatgaagtgt ggcccgccat
     2041 caggcagttg ctgccagact gaaccactac cgagaatcta acgactcgaa cacccgcccg
     2101 gaatacgcta caatggttcc ctggggtcga tccggcttcg acgtgggtca cgacacagcg
     2161 cagggcatgc cgagcaccag taagctcgta aatccactgg aaaactacaa acgccaacga
     2221 cgagcgtttc gctctagccg cttaattgcg gtgagccgct g
//
LOCUS       DASTNK010000075        25315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1350037, whole genome shotgun sequence.
ACCESSION   DASTNK010000075 DASTNK010000000
VERSION     DASTNK010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 25315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25315
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350037"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1481)
                     /locus_tag="VFR20_05685"
     CDS             complement(<1..1481)
                     /locus_tag="VFR20_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF490 domain-containing protein"
                     /protein_id="HEU0230041.1"
                     /translation="MNALWRWPRRVAIWAGPTLVLLLAVACGFLYWVIRSPAGTRWVL
                     TTAVAQLGGQIQGVSGSLWEGVGVQALSLDMADFSLRLAGAQVRVAWPDLIEHRLHGV
                     DVSADSVDLNLRTPARPAPDTGPFSMPVVPLAIVLDRLSVGRFALSRNGKPVPVAVNG
                     LDGSFSLLDGKARLDLRHVDVSSQGMRVAAHGDVTLLDLRQPWPLDAHLILDLSGHGK
                     DSPLCVRRYVPTLPAIKQTSPESGGCAATIDVKAQGTADQLAVTVAGRGQSLNLDASA
                     NLTPTGVFPLRSATIELGLADGSLLKGRFDWASQVENGVVRDHVTGSLQARKLDVKAL
                     AGASLPPAILTMSADFDARLRDHRVPQSMVLNVVFDKGSSWNRQPLSGHLKAEAAQRE
                     VVDRAPASAPSWQNLKIEGLDLDLLLGADHIKAKGALGEPRNRLDLDLRAPNLAAFWP
                     GLPGGLSLKGVVGGDVGSHQADLVATYTPAAQKAQRGAAPHGG"
     gene            complement(1478..3229)
                     /locus_tag="VFR20_05690"
     CDS             complement(1478..3229)
                     /locus_tag="VFR20_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEU0230042.1"
                     /translation="MALATSLCAKGYAASAPEVIIDPGGVPPAALRSITSAVNVITRL
                     ATDQDGGELSRLRRRAREATLSALETQGYFSPKVTLDVGTDIGGETWNITIDPGRRTT
                     VNSVHLAFAGGIAAPAYAERIEALREAWPLKKGKPFINADWSNAKTALLDGVASRDFF
                     FARLANSEARVTAATARADLNVDIESGPRVRLGKLRTSGLKRVPASLIKRYVRYSPGD
                     PYSQQQLDEWQQALQSTSFFRGAFVTLDGDITQGTYLPNGDLELPVQVRVSEAPARSV
                     TGSLGVDSDNGVRVEGLYRQNIVFGKPVWIETGVGVDKNQQRLFYDVHLPPTVSGYKD
                     SFGTLYQHSNIEGVDNQRVAVGWKRRQERTSSGSRIQYETQWALVAAYDKTRISGAPD
                     YNVPSLVATWDWLRRDVNDKYDPREGNLVDFGVGAGLTLDKHEPFYRASLRAQKWWPI
                     GRRDVLTVRGQVGKVWSQTSRLPDDFGYRTGGAHTIRGYRYLSIGLARGNATVGAPAL
                     AVGSIQYTHYLNQHFGVEAFVDAGDAAESFGQMRLHLGYGVGGVVRTPAGPFSVDVAY
                     GQYDHSLRLVFSMGVAF"
     gene            complement(3256..4350)
                     /gene="apbC"
                     /locus_tag="VFR20_05695"
     CDS             complement(3256..4350)
                     /gene="apbC"
                     /locus_tag="VFR20_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEU0230043.1"
                     /translation="MNPNTEQIRAALAEVTDPRTDLRLDIAEDGIRLDNTDLCLTVTL
                     GYPLHNGQALLRKRIETAIHPLGVVLKDLVCRVVIAAHAVQKTLHRLPNVRNIIAVAS
                     GKGGVGKSTTAVNVALALMAQGATVGLLDADIYGPSVPMMLGLSGRPKSLDGKTMLPM
                     SAHGLQANSIGFLVDSDAPAIWRGPMVTQALQQLLSLTNWNDLDYLIVDMPPGTGDIA
                     LTMAQKVPLTGAVIVTTPQDLALADARKGLRMFQKVDVPVLGVVENMSVHICSNCGHV
                     EPIFGQDGGRDLAAEFNLPWLGGLPLVMAIRTQTDSGNPTVVAEPDGEIATLYHDVAH
                     KLAVGVARLPLDQAGKKPSVVARAVGESGA"
     gene            4450..4698
                     /locus_tag="VFR20_05700"
     CDS             4450..4698
                     /locus_tag="VFR20_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230044.1"
                     /translation="MNTFNQLLRGVLFLVLAVFGMAMAVIFMISTTIAIGVLYVVARI
                     KGRPFGVKAYWDQRRHPGAQKNTFKNKDVVDVEMREIH"
     gene            4912..6987
                     /gene="metG"
                     /locus_tag="VFR20_05705"
     CDS             4912..6987
                     /gene="metG"
                     /locus_tag="VFR20_05705"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU0230045.1"
                     /translation="MPRTVFVTTALPYANGSFHLGHILEYIQADIWVRSMRMAGHTVH
                     FVGADDAHGAPIMLKAESEGITPRELVARYASERPLYLNSFHIAFDHWHSTDSAENVA
                     LSQGIYTQLKAAGFIATRTIEQFYDPVKGMFLPDRYIKGECPRCHTKDQYGDACENCG
                     AVYAPTELIEPYSTLTNAKPVLKSSEHFFFKLSDPRCVAFLRQWTTGINSQGRKRLQS
                     EVLAKTREWLGGDEDPDTHMADWDISRDAPYFGIEIPDTPGKYFYVWLDAPVGYLASL
                     EAYCGKTGLDFQALIDPNGTTEQVHFIGKDIVYFHALFWPAMLKFAGRKTPDSINVHG
                     FVTVDGQKMSKSRGTGISPLRYLDIGMNAEWLRYYFAAKLNARVEDVDFVANDFVTRI
                     NSDLVGKYVNIASRAANFINKHFDGALGYAGDTGALRTELADTAREVQELFESRDYSR
                     AVRRIMAHADAINQAFDTAQPWVKAKGIATAPDEQKTELQDTCSRALAGFKALTVMLA
                     PVLPALAQRVASELFGEGDGYLWTDAAKLPTRVSPFKHLMQRVDPKVFEELFDKPEEP
                     APAPVPGGEAIADVIDIKDFSKIDLRIAKIVDCSEVEGSDKLLRLSLDVDEGRLRQVF
                     SGIKSAYAPQDLIGKLTVLVANLAPRKMRFGVSEGMVLAASHRDEKADGGIYILEPWP
                     GARPGMRIN"
     gene            complement(6980..8194)
                     /gene="kch"
                     /locus_tag="VFR20_05710"
     CDS             complement(6980..8194)
                     /gene="kch"
                     /locus_tag="VFR20_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HEU0230046.1"
                     /translation="MRVVWLRNQIRRLYAAFPLHWFVAVLVAIDGFTFLQPGVSAVRA
                     AQPQSWLSWFSFEHLRLFLDSVGLVEIPRLILGVSLQIMAIGLILRARIAWAFSLLLL
                     IVTGTIALWSGGHDHLGLAAYTLALVLMLIAYWRRFDRSSLAAGSLFAVLSILSLLVY
                     AVFGALYLGDEFQPPIHDIVSALYFAVVSMSTVGYGDITPHTASSRLFTVSIIIMGIT
                     VFATSVSAIIGPIIGGNLKRLVKGRLNNVMRKNHIIIAGATPLAQSVYKGMRARGFDV
                     TVIVPEDVSHVYPEGADLIVGDPSEESALTEAGAAKAKYILALRNDDAENAFIVLAAK
                     EVGGPDTKTVALANTTTHLEKIKRVGPDMVLSLQLLGGEVLSRTLSGEPLDSEMISHM
                     LFAETAAPNVVS"
     gene            8461..9426
                     /locus_tag="VFR20_05715"
     CDS             8461..9426
                     /locus_tag="VFR20_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEU0230047.1"
                     /translation="MTPGRPSASPDDRLTPGGRSDDDAQRHAAGRRSTWASVAVNVCL
                     IIMQATIGTLAHSQALIADAIHSLSDLLSDGIVLVAGKHSHKAPDADHPYGHLRFETA
                     ATLALGAILLAVGAGMLWNAFEHLQQSPESDGRVSAAALVAAVITLCAKEGLFRYMLG
                     VAKRARSSMLVANAWHARSDAASSLVVAVGIIASLAGFPMADPLAALIVGVIVGRMGW
                     KFFLSAFNDLTDRAVDEETEARIRAHILATDGVLGIHGLKTRKLGDMIWVEVDLEMDA
                     DLTIAQGHAIAEAARANVLRHEPVLDVMTHFDPVRVTSDGHQTRR"
     gene            complement(9503..10159)
                     /locus_tag="VFR20_05720"
     CDS             complement(9503..10159)
                     /locus_tag="VFR20_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0230048.1"
                     /translation="MGRPREFDEAEVLDAAVACFWEHGYEATSTRELAGRMGLTGASL
                     YNAFGGKRALYRKALDHYIDASFADRVRRLEGTLPPLEAIRAFFMEIIRRSLDDPRRK
                     GCMLINSALEVAPHDPEFRRIVTEILAQIEAFFRRCVRAGQRDGSITRSQSATDFGRA
                     LLAAHIGVRVMARTRPEKPLLEGMVRPILAVLDAEAGRRPHSGPRMTSQPQDDKPASG
                     "
     gene            10389..11093
                     /locus_tag="VFR20_05725"
     CDS             10389..11093
                     /locus_tag="VFR20_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0230049.1"
                     /translation="MIRFYYHPTPNPAKVALFLEETGLAYEVVPVDTSKGQQHDPAFR
                     KINPNGKVPAIVDMEGPGGQPATVFDSTAILLYLAEKAGRLLGQPHDRPELLSWLLFL
                     GSGLGPFSGQAVHFQYAAPPGLDYAVNRYRREAERHYHVLDDHLAGREFIVGNEYTIA
                     DISAWGWLDRASRVMKGTEDPLGAYPDIKRWFQAIDARPAVARARQVGAGLAFKTVND
                     EETRRALFPSNYPAAG"
     gene            11190..11867
                     /locus_tag="VFR20_05730"
     CDS             11190..11867
                     /locus_tag="VFR20_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEU0230050.1"
                     /translation="MSATKAIVFDLYGTLYDVHSVIEQCDCAYPGKGAEISLLWRQKQ
                     LEYTWLRSLMGQRLYIPFEQATRDALVYTCGHLGVDLDDTTCAALCDAYLKLSPYPEV
                     PAALARLQSMGLPLAILSNGSAFSINSVVNHSGLQARFTHLLSVDPARTFKPDPRVYT
                     LACEAFSLPADQILFVSSNAWDASGAYHFGFDVCWVNRRGAVFDELGAAPRAIVKGID
                     EIPALVG"
     gene            complement(11917..13401)
                     /locus_tag="VFR20_05735"
     CDS             complement(11917..13401)
                     /locus_tag="VFR20_05735"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU0230051.1"
                     /translation="MTVPIFRLALSCGATGLVRWGQTLAAVGLALLANSCVHAKDLAQ
                     PPDTLLAGRQEAGRSPGTGSRFCAQGIPGRMPVSLLDAVGRALCSDPRTRQAWAYIQT
                     QQAQLNVDRAAYWPTLSANASVDRVDQRVTYTNLPGLESSLRTRSTDVGLTLSWVLYD
                     FGLRAANLEHSRQLLNAAIASRNETLQTVFLDTVRVYYDAQTRLTLLRADRETERIAH
                     ESFVIAQAKFKAGAATLADKLQAKTAYSQAVLERMQAQSDLDKAMGALAVEMGLAPGV
                     SLHLPSVDDDGVHQDDYRKTVDTLVGEAMHTNPSVLAARAKLEAARAEARSARSQSLP
                     RLSLMASMDRSDTPITQVTSRQVINNRSIGLQLTIPIVDVGSHYQISKADAEVDARKA
                     DLAQARRTVSLDVWKSYEEFTSGLATLETANDLLHSARQSVKVAFGRYKAGVGSLLEL
                     LRAQSDLTNAERQRVLALTQWKTGQFKLAASLGQLERGGLPRAR"
     gene            complement(13398..15548)
                     /locus_tag="VFR20_05740"
     CDS             complement(13398..15548)
                     /locus_tag="VFR20_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEU0230052.1"
                     /translation="MLEHLSFGLRRRVPLLLQTEAAECGLACLGMVAGFHGFHVDLAA
                     LRSRFPTSLKGVTLAMLLRIAAQLKLATRPLKLDLDGLSSLRLPCILHWDLNHFVVLR
                     EVGRNFVVVHDPATGVRKYSMEEVSRSFTGIALELWPDAGFQEAQARRPVRLRQLLGR
                     VTGLKRSFTQIILLALTLEVFNVVNPFFLQWVIDHVLVSVDRSLLTTLALGFGLLLLF
                     QQAASAARGWMLMYVGTTLNVQWRANVFGHLLRLPMEYFEKRHLGDVVSRVGSVDAIQ
                     RTLTTSFVEALLDGVMTVVTLLLMFLYSPALAGIAVGAMALYALSRWAWYAPLRNATE
                     ERIVHAAKQQTHLLESIRGARTIKLFQHQEQRRSTWLSLLVNQINADLTTQKLTLLYK
                     TLNGVLFGIENILIIWLGARFVLDGAFTVGALIAFNAYKGQFDTRVSGLLDKWVDVRM
                     LQLQGERLADIVFHPSESEDGGEVGEEDVSAQPSIEARNLRFRYGDQEPYVLDGVSFR
                     IAAGESVAITGPSGSGKSTLIALLLGLLPPTEGEILVDGKPTQRLGSGTLRRLVGTVL
                     QDDVLFAGSIAENICFFDVQPDQARMRRSAELAAIHDDIVAMPMGYNTLVGDMGTVLS
                     GGQKQRVLLARALYKQPRILFLDEATSHLDIERERQVNAAVLTLQMTRVIVAHRPETV
                     ASAGRCIVLVGGRIVYDGPAVPARGGAAAVADAS"
     gene            complement(15552..16802)
                     /locus_tag="VFR20_05745"
     CDS             complement(15552..16802)
                     /locus_tag="VFR20_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEU0230053.1"
                     /translation="MSLFRKEALEARQTPWLGDIVLVRPVSFSIVVAVAVAAAVAVLL
                     FLVLGTYTARETVSGQLVPDSGVITLRAPRAGVVVERRVRKGQKVKAGDVLYVVSTDT
                     RSQLGSTQAAISAQVGRREASLRAEQQRTRQLHKSERTSLVTKISALAAELQTLDAQI
                     KGQRARLALARQSVDRYRKLVAQGYVSREQLEARQQDALDQGNQLQSLQRDRIRMSEQ
                     LSAQRADLAELALKQQNEAARFERDLTNAAEALSESEAKRRELITAPVTGSVAAVLAD
                     PGQAIGGDRPLATIVPAGSKLVALLYAPSKAVGFVRPGDRVQIRYQAYPYQTYGQQPG
                     TVLSVAHTALSASELRGGAVSGQSDTESLFLIRVRLASQTDSARGQALALLPGMALEA
                     DIMLQKRRLYQWALKPLYSLSDGF"
     gene            complement(16984..17148)
                     /locus_tag="VFR20_05750"
     CDS             complement(16984..17148)
                     /locus_tag="VFR20_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230054.1"
                     /translation="MTQYQDNKESLVQELTVSEIEQVSGGMAPWDYHGAFSYCSLYTG
                     GPFEYLQCRF"
     gene            complement(17530..18825)
                     /locus_tag="VFR20_05755"
     CDS             complement(17530..18825)
                     /locus_tag="VFR20_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEU0230055.1"
                     /translation="MPVSPSLDQMNASVAVPALGGWWRHLLAFAGPGYLVSVGYMDPG
                     NWATDLAGGSRFGYLLLSVVLLSSLMAMLLQAFAARLGIATGLDLAQACRARYRPSAS
                     LLLWVACEAAIIACDLAEVIGTAIALNLLFGIPLVLGAVLTALDVFVVLLLMQRGFRR
                     LEAFVMTLLLIIAVCFGIQIVLARPDVAGLVKGFMPQARIVTDPAALYLAIGIIGATV
                     MPHNLYLHSAIVQTRDYPRTEAGRREGLRWAVADSTIALLLALFVNAAILITAAAVFH
                     AAGRTDVEEISQAYHLLSPMLGVGLASWVFAVALLASGINSTVTATLAGQVVMEGFLG
                     LRLPGWAQRLLTRGLAIVPVVIITSLYGEGGTARLLVLSQVVLSMQLPFAVIPLVRFV
                     SDKKMMGAFAIGKAATMLAWAVAVLICALNVKLLLDSVL"
     gene            18996..19478
                     /gene="mntR"
                     /locus_tag="VFR20_05760"
     CDS             18996..19478
                     /gene="mntR"
                     /locus_tag="VFR20_05760"
                     /inference="COORDINATES: protein motif:HMM:NF008273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HEU0230056.1"
                     /translation="MTKTRTPTMFQRTLVPPEVHAEGFVQARHAHRQELAEDYVELID
                     DLIHDGGEARQVDIAARLGVAQPTVARMLKRLDSQGLVSLRRYRGIALTEAGQALAEA
                     SRKRHQLCESLLLALGVGADAARRDAEGIEHHVSEETLKAFSRFLARERGKTTRAGQA
                     "
     gene            complement(19568..20041)
                     /locus_tag="VFR20_05765"
     CDS             complement(19568..20041)
                     /locus_tag="VFR20_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEU0230057.1"
                     /translation="MNIWVDADACPAVIKDILYRAAQRSQRPLTLVANQMLRTPPSPL
                     IRAVQVPRGFDVADDYIVEHAAAGDLVITADIPLASEALRKGALVLSPRGERYTADSI
                     GERLSMRDMMEELRSAGVDTGGPAAFSQADRRAFANALDQLLQKALREDTAGRVR"
     gene            complement(20061..20333)
                     /locus_tag="VFR20_05770"
     CDS             complement(20061..20333)
                     /locus_tag="VFR20_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230058.1"
                     /translation="MKKTDLEKQKALKLMGKLNAAVPPGRFAGAEPVVDRREQRRLDQ
                     AAGLVSFPVKLRQPLISALNAKAQADGVSVNELVNTLLAGALKGAI"
     gene            complement(20535..20608)
                     /locus_tag="VFR20_05775"
     tRNA            complement(20535..20608)
                     /locus_tag="VFR20_05775"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20574..20576),aa:Gly,seq:ccc)
     gene            20749..21516
                     /gene="trmB"
                     /locus_tag="VFR20_05780"
     CDS             20749..21516
                     /gene="trmB"
                     /locus_tag="VFR20_05780"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU0230059.1"
                     /translation="MTSMTSTTDPTSNGPATPAASAAPAAAHSDHGHIRSFVHRRSHI
                     TPAQKEALDRLMPQWAIPYQPRPLDFATAFGRAAPVVLEIGFGMGETTEQIATARPGD
                     NFLGVEVFNAGVGGLLKRIESSGLTNIRIIQHDAVEVLRDMIPVDSLAGVHIYFPDPW
                     PKARHHKRRLIQPPLVALLASRIQPGGYLHCATDWENYAEQMLAVLSGEPLLRNTCDG
                     YAPRPEYRPQTKFETRGLRLGHSVRDLIFTRVAPTAS"
     gene            21605..22540
                     /gene="pip"
                     /locus_tag="VFR20_05785"
     CDS             21605..22540
                     /gene="pip"
                     /locus_tag="VFR20_05785"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEU0230060.1"
                     /translation="MYPEIEPYRHGMLDTGDGHQVYWELCGNPAGKPAMFLHGGPGGG
                     CSVNHRRQFNPERYNVLLFDQRGCGRSTPHASLENNTTWDLVADMERLRTEVLDAEQS
                     LIFGGSWGSTLALAYAETHPQRVSELIVRGIFTLRRDELLWFYQEGASWLFPDLWEHY
                     LAPIPVDERGDMISAYRRQLISNDRQTQLRAAHAWTQWESRTITLLPSMAHQQSHAED
                     DSALAFARIENHYVAHGGFMEDGQLLRNAHKLRDIPGVIVQGRYDVCTPARTAWDLHK
                     AWPQAEFHLIDDAGHAFDEPGILTELLAATDKFSR"
     gene            complement(22581..23996)
                     /gene="ffh"
                     /locus_tag="VFR20_05790"
     CDS             complement(22581..23996)
                     /gene="ffh"
                     /locus_tag="VFR20_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU0230061.1"
                     /translation="MLDNLTQRLSHVVKTMRGQARLTEANTRDMLREVRMALLEADVS
                     LPVVRDFVERVRESAMGQEVVGSLNPGQALVGVVHKQLTALMGEDLGPEAGELSLAMQ
                     PPAIVLMAGLQGAGKTTTTGKLAKWLAEGGHVQHGRKTGKKKVLVTSADVYRPAAIEQ
                     LKTVAEQAGVEFFPSDGSQKPEDIARTALDHARRHHFDVLIVDTAGRLGIDEAMMREI
                     RALYDLLHPIETLFVVDAMQGQDAVNVAKAFADALPLTGVVLTKLDGDARGGAALSVR
                     HVTGKPLKFVGVSEKLDGLEPFYPERMAQRVLGMGDIVSLVEQAQKNIDIAEAQKLAT
                     KLKSSGRFDLNDFRDQLQQVKKMGDMGSLLEKLPSQFAQAAGQLQSGQADKQLRRTEG
                     ILNSMTAQERAKPALLKASRKRRIAAGAGVPVQEVNRLLTQFEQMQGMMKQMKKGGMA
                     KMMRAMGNLKGMGRGGFGGMH"
     gene            24098..24940
                     /gene="ccsA"
                     /locus_tag="VFR20_05795"
     CDS             24098..24940
                     /gene="ccsA"
                     /locus_tag="VFR20_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEU0230062.1"
                     /translation="MSAGIVFHLLAAIAYAILAISLWRPLSRGGTEAKAGKVGRICLL
                     SAIILHGIALQQSLLPGHSVHLGLAPAVSAALWLGMIVFWLESLLVRIDPLLLILLPV
                     VAVVCLLSAIFPETHAIAHAGSDLLRLHLLIALSAYGLITVAALHAILMTALDRHLHR
                     PVTQAAQGNSLMRALDSMPPLLVQEHLLFRLIWIGFAVLTLAVISGAMVSMRLIGVPF
                     PLDHKTVFTLLSWFTFGGLLAGRYIRGWRGRIALRWTLVGFVFLLLAYTGSRFVLEVL
                     LHRG"
     gene            24942..25199
                     /locus_tag="VFR20_05800"
     CDS             24942..25199
                     /locus_tag="VFR20_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HEU0230063.1"
                     /translation="MEKLFSWVLLIVVGLVIARILSRQNAARQHMATRQAPRRGAPPS
                     IAPEAMVRCAHCGIHLPRSEAVMVQGKTWCSEEHARLGPRQ"
BASE COUNT         4847 a         8520 c         7535 g         4413 t
ORIGIN      
        1 ttgccaccat gcggcgcggc gccacgttgc gccttttgcg cagcgggcgt gtacgtggcg
       61 accagatcgg cttgatgact gcccacatcc cctccaacca ctcccttgag gctcaatccg
      121 cccggcaggc ctggccagaa cgccgccaga ttcggtgccc gcaggtccag atccagccgg
      181 tttctgggct cgcccaaagc gcctttcgcc ttgatgtgat cggcccccag gagcagatcc
      241 agatccaggc cctcgatctt caggttctgc cacgatggcg cactggccgg cgccctatcg
      301 accacttcgc gctgcgcggc ctcggccttc aagtgaccgg ataacggctg gcggttccat
      361 gacgaccctt tgtcgaagac gacattgagc accatggatt gtggaacacg gtggtcacgc
      421 aaccgggcgt cgaaatccgc cgacatggtc agaatcgccg gtggcaagga tgcccctgcc
      481 agcgctttca catcgagttt ccgggcctgc aacgaccccg tcacgtgatc gcgcacgaca
      541 ccattttcca cttgggaagc ccagtcgaac cggcccttca gaagcgagcc atccgccagc
      601 cccagttcga tggtggcgct gcgcaacgga aaaacaccgg tgggcgtgag gttcgcgctc
      661 gcatccagat tcaggctttg accacgcccc gccactgtca cggcgagttg atcggccgtc
      721 ccttgcgcct tcacgtcgat tgttgcggca cacccccccg attccgggga cgtttgcttg
      781 atggcaggca gcgtggggac gtagcggcgc acgcatagcg gcgaatcctt gccgtggccg
      841 ctcaggtcca gaatgaggtg tgcatccagc ggccacggct ggcgcagatc cagcaatgtc
      901 acatcaccgt gcgccgcaac ccgcattccc tggctcgaca cgtcgacatg cctcaagtcc
      961 agccgggcct tgccatcgag caatgagaac gacccgtcca atccattgac ggccaccggc
     1021 acgggtttgc cattccggct gagcgcgaaa cgccccaccg acagacgatc cagcacgatc
     1081 gccaacggca ccaccggcat ggagaacggc cccgtgtccg gagccggccg cgccggggtg
     1141 cgcaggttca gatccacgga gtcggccgac acatcgaccc catgcaggcg atgttcgatc
     1201 aggtcgggcc atgccacccg cacctgcgcg ccggccaggc gcaacgagaa atcggccatg
     1261 tcgagggaca gtgcctggac ccccacccct tcccacagcg aaccgctcac accctggatt
     1321 tgcccgccca gttgcgccac agctgtcgtc agcacccagc gggtgcccgc gggcgaacga
     1381 atcacccagt acagaaagcc gcaggccacg gcgaggagca ggaccagcgt ggggcccgcc
     1441 caaatggcga cccggcgcgg ccagcgccac aaggcgttca aaatgcgacc cccatggaaa
     1501 acaccagacg gaggctatga tcatattgac cataggccac atccacagag aacggcccgg
     1561 cgggtgtgcg caccacgccg cccacgccat atcccaggtg cagcctcatc tgcccgaacg
     1621 attccgccgc gtcgcccgca tcgacgaagg cctccacacc gaaatgctgg ttcagatagt
     1681 gtgtgtactg gatgctgccc accgccaacg ccggcgcacc gaccgtggcg ttcccccgcg
     1741 cgagaccgat gctcagatag cgatagcccc gtattgtgtg cgcgccaccg gtgcgatagc
     1801 caaagtcgtc cggcaagcgc gacgtctgcg accagacctt gcccacctgg ccacgcactg
     1861 tgagcacgtc gcgccgcccg atgggccacc acttttgcgc gcgcaggctg gcgcggtaga
     1921 agggttcgtg cttgtcgagt gtgaggcccg cccccacgcc gaaatcgacc aggttgcctt
     1981 cacgcggatc gtacttgtcg ttgacatcgc gacgcagcca gtcccacgtc gccaccagcg
     2041 acggcacgtt gtagtcaggc gcgccggaga tccgcgtctt gtcgtatgct gccaccagcg
     2101 cccattgcgt ctcgtactgg atccggctgc cggaagacgt gcgctcctgg cggcgcttcc
     2161 atcccaccgc gacgcgctgg ttgtccacgc cttcgatgtt ggaatgctga tagagcgtgc
     2221 cgaaactgtc tttgtagccg ctcaccgtgg ggggcagatg cacatcgtag aacaagcgtt
     2281 gttggttctt gtcgacaccc acaccggttt cgatccatac cggtttgccg aacacgatgt
     2341 tctgacgata caagccttcc acccgcacgc cattgtcgct gtcgacaccc agcgagccgg
     2401 tcacgctgcg cgcgggggcc tcggagacac gcacttgcac aggcagttcc aggtcgccat
     2461 tgggcaggta cgtgccctgc gtgatgtcgc catccagggt gacgaaggcg ccgcgaaaga
     2521 acgatgtgga ctgcagcgcc tgctgccatt cgtccagttg ctgctggcta tatgggtcgc
     2581 caggggaata gcgaacgtag cgtttgatga gcgaagccgg aacccgcttt aaacccgatg
     2641 tcctgagctt acccaggcgc acgcgcggcc cgctctcgat gtccacattg agatcggccc
     2701 tggccgtggc cgccgtcacc cgggcctcgg aattggccag gcgcgcgaaa aaaaaatccc
     2761 gcgaggccac accatcgaga agcgccgtct tcgcgttgct ccagtcggcg ttgatgaacg
     2821 gcttgccctt cttgagcggc cacgcctcgc gcagcgcttc gatgcgctcg gcgtacgctg
     2881 gcgcggcgat cccgcccgcg aacgccagat gcacgctgtt caccgtggtg cgcctgcccg
     2941 gatcgatggt gatgttccag gtttccccac caatgtccgt gccaacgtcc agcgtcacct
     3001 tgggcgaaaa atacccttgt gtctcgagcg cggacagtgt cgcctcgcgc gcgcgtcggc
     3061 gcagacgcga cagctcgccg ccgtcctgat cggtggcgag ccgtgtgatg acgttgaccg
     3121 ccgacgtgat ggaccgcaac gcggcgggcg gcacaccgcc cggatcgatg atgacttcag
     3181 gcgccgaggc cgcatacccc ttggcacaca ggctcgtggc cagtgccaag gcgagtagcg
     3241 gtcggcgcgc acgcactacg cgccgctctc gcccaccgcc cgggcgacca cggacggctt
     3301 cttgcccgcc tggtccaggg gcagcctggc cacgccgacc gccagcttgt gcgccacatc
     3361 gtggtacagc gtggcgatct cgccatcggg ttcggccacc acggtggggt tgcccgaatc
     3421 ggtctgggtg cgaatggcca tcaccagcgg cagcccgccg agccatggca gattgaattc
     3481 ggccgcgaga tcgcgcccgc catcctgtcc gaagatgggt tcgacatgac cacaattcga
     3541 gcaaatgtgc accgacatgt tttcgaccac gcccagcacg ggcacgtcca ccttctggaa
     3601 catgcgcaga cctttgcggg cgtccgccag ggcaaggtct tgcggcgtgg tcacgatcac
     3661 cgcgccggtg agcggcacct tttgcgccat ggtcagcgcg atgtcgccgg ttcccggcgg
     3721 catgtcgacg atgagatagt cgaggtcgtt ccagttcgtc aggctcagca actgctgcag
     3781 ggcctgcgtg accatgggcc cgcgccagat ggctggcgca tcgctgtcga caagaaaacc
     3841 tatggagttg gcctgcagtc cgtgagcgct catgggcagc atggtcttgc catccaggct
     3901 tttcggacgc cccgaaagac ccagcatcat gggcacgctg gggccataaa tgtcggcatc
     3961 gagcaggccc acggtggcgc cctgcgccat cagggccagc gccacgttca cggcggtggt
     4021 gctcttgccc accccgccct tgcccgaggc cacggcgatg atgttgcgca cgttgggcag
     4081 gcgatgcagc gtcttctgca cggcatgcgc ggcgatgacc acgcggcaga ccagatcctt
     4141 cagcacgaca cccagagggt ggatcgcggt ttcaattcgt ttccgcaaca gggcctgccc
     4201 attgtgcaat ggatagccga gcgttacagt aaggcatagg tcggtattat ccagccgtat
     4261 gccgtcctcg gcgatatcca gccgcaaatc ggtcctgggg tctgtgactt ccgcgagcgc
     4321 ggcacgtatc tgttcagtgt tcgggttcat aactaaggac tttccgcctg gaaaattgac
     4381 gacctcgcca agcctcaagg atagcggatt ggcgcaacat ttttccggag atggtatcca
     4441 ctaaacgcaa tgaacacatt caatcaacta cttcgcgggg tgcttttcct cgtgctcgcg
     4501 gtgttcggca tggccatggc tgtcattttc atgatttcca cgaccattgc aatcggcgtg
     4561 ctgtacgtcg tggcccgcat caagggacgc ccgttcggcg tcaaggccta ctgggatcag
     4621 cgtcgccacc ccggcgcgca gaaaaacacc ttcaagaaca aagacgtcgt cgacgtcgag
     4681 atgcgcgaaa tccactgata acggccgtag ccggccgcca cgccactgtt ggctacgagt
     4741 ggtaactctt cgttaacccg cagccctgtc tccttgctcg cccccgccta ccggcggcgg
     4801 cggacgcctt tgccgcaaaa gtcctaaaat acagggtttt ccgcctccca caggggttcc
     4861 cgccccatcg ggcggatcga tcccacttac ctcgtcacgc ccactgcccc catgccgcgc
     4921 acagtcttcg tcaccaccgc cctgccctat gccaatggct cgttccacct tggccatatc
     4981 ctagaataca tccaggccga tatctgggtg cgctcgatgc ggatggccgg ccataccgtg
     5041 catttcgtgg gcgccgacga cgcgcatggc gcccccatca tgctgaaggc ggaaagcgag
     5101 ggcatcacgc cgcgtgaact ggtggcgcgc tacgccagcg aacgcccgct gtacctgaac
     5161 agtttccata tcgccttcga tcactggcac tccaccgatt cagcggagaa cgtcgcgctg
     5221 tcccagggca tctacaccca attgaaggcg gcgggcttca tcgccacccg caccatcgaa
     5281 cagttctacg acccggtcaa gggcatgttc ctgcccgatc gctacatcaa gggcgagtgc
     5341 ccgcgctgcc acaccaagga ccagtacggc gacgcctgcg agaactgcgg cgcggtgtac
     5401 gcgcccaccg aactcatcga gccatactcc accctgacca acgccaagcc ggtgctgaaa
     5461 tcgtcggagc acttcttctt caagctgtcc gacccgcgtt gcgtggcgtt cctgcgccaa
     5521 tggaccacgg gcatcaacag ccaggggcgc aagcgcctgc agagcgaagt gctggccaag
     5581 acccgggaat ggctgggcgg cgacgaagac ccggacaccc acatggccga ctgggacatc
     5641 tcgcgggacg ccccctattt cggcatcgaa attcccgaca cgccgggcaa atacttctac
     5701 gtgtggctgg acgccccggt gggctatctg gcgtcactcg aggcctattg cgggaagacg
     5761 ggcctggatt tccaggcatt gatcgacccg aacggcacca ccgagcaggt gcacttcatc
     5821 ggcaaggaca tcgtctactt ccatgccctg ttctggccgg ccatgctgaa attcgccggg
     5881 cgcaagaccc ctgacagcat caacgtgcat ggcttcgtca ccgtggacgg ccagaagatg
     5941 tccaagagcc gcggcaccgg catctcgccg ctgcgctacc tcgacatcgg catgaacgcg
     6001 gaatggctgc gctactactt cgccgccaag ctcaacgcgc gcgtcgagga tgtggatttc
     6061 gtcgccaacg acttcgtcac ccgcatcaac agcgacctgg tcggcaagta cgtcaacatc
     6121 gccagccgtg ccgccaattt catcaacaag catttcgacg gcgcgttggg ctatgcgggc
     6181 gacactggcg cactgcgcac cgagctggcc gatacggcgc gcgaagtgca ggaattgttc
     6241 gaatcgcgcg actacagccg cgcggtgcgc cgcatcatgg cgcatgccga cgcgatcaac
     6301 caagccttcg acaccgccca gccgtgggtc aaggccaagg gcatcgccac agcgcccgac
     6361 gaacaaaaaa ccgagctgca ggacacctgc tcgcgcgcgt tggccgggtt caaggccctg
     6421 acggtcatgc tcgcccctgt gctgcccgcg ctggcgcaac gcgtggcgtc ggagcttttc
     6481 ggcgaaggcg acggctatct gtggaccgat gccgccaagc tgccgacgcg cgtcagcccg
     6541 ttcaagcatc tcatgcagcg cgtcgatccc aaggtgttcg aagaactgtt cgacaaaccc
     6601 gaggaacccg cccccgcgcc ggttccgggt ggcgaggcca tcgccgacgt catcgacatc
     6661 aaggactttt ccaagatcga tctgcgcatc gccaagatcg tcgattgcag cgaagtcgag
     6721 ggctcggaca aactgctgcg cctgagcctg gatgtggatg aaggtcgcct gcgacaggtg
     6781 ttctcgggaa tcaagtcggc ctatgcgccg caagacttga tcggaaaact gacggtgctg
     6841 gtggccaacc tggcgccgcg caagatgcgt ttcggcgtgt cggagggcat ggtgctggcc
     6901 gccagccacc gcgacgaaaa agccgacggc ggcatctaca tactcgaacc ctggcccggc
     6961 gctcggcccg gcatgcgcat caactgacca cgttcggcgc ggccgtctcg gcaaacagca
     7021 tatgggaaat catctcgctg tcgagcggct cgccgctgag tgtgcgtgac aacacttcgc
     7081 cacccagcaa ctgcagcgac agcaccatat ctggccccac gcgcttgatc ttctcgaggt
     7141 gcgtggtcgt gttcgccagc gccacggtct tggtgtcggg tccgcccact tcttttgccg
     7201 ccagcacgat gaaggcattt tcggcgtcat cgttgcgcag ggccagaatg tacttagcct
     7261 tggcggcacc cgcctcggtc aacgccgatt cctcggacgg atcgcccacg atcaggtcgg
     7321 ccccttcggg gtagacgtgg ctcacgtcct ccggcacgat gacagtgacg tcgaagcccc
     7381 gcgcgcgcat gcccttgtag acactctggg ccaggggcgt agcgcccgcg atgatgatgt
     7441 ggttctttct catgacattg ttcaatcgcc cctttacaag gcgcttcagg ttgccgccga
     7501 tgatcggccc gatgatcgcg ctcaccgacg tggcaaacac ggtgatgccc atgatgatga
     7561 tggagaccgt gaacagccgg ctcgacgcgg tgtgaggggt gatatcgcca taccccacgg
     7621 tggacatcga cacgacggcg aaatacagcg ccgagacgat gtcatggatg ggcggttgga
     7681 attcatcccc caggtacagc gcgccaaaca ccgcgtagac cagcaacgac aggatgctca
     7741 ggacagcgaa aaggctgccc gccgccaagc tggagcgatc gaaccgccgc cagtaggcga
     7801 tgagcatcaa caccagcgcc agggtgtagg cggccaggcc tagatggtcg tgtccgccgc
     7861 tccagagcgc gatggtgccc gtgacgatca acaacagcaa tgaaaacgcc caggcgatgc
     7921 gggcgcgcag gatcaggccg atggccatga tctgcagact cacgccgaga atcagacgtg
     7981 ggatctcgac cagccccacc gagtcgagaa agaggcgaag gtgctcgaac gagaaccacg
     8041 acagccagct ctggggttgg gccgcgcgca ccgccgacac ccctggctgg agaaacgtga
     8101 atccgtcgat cgccaccagt accgccacaa accaatgcag cggaaaggcc gcgtatagcc
     8161 gccgtatctg attgcgcagc cagacaactc tcaaacggga atcctcacac ggcgatagca
     8221 gcgacgatga tgacgacgtg tcatcgccgg cgccggatgg accggggcct cttccgcatt
     8281 gactcgccgg ggcagggttt cattcaaaga gaacagggcc gggcagacga tcaaaacggg
     8341 aagaggcggc gaacctcaag tttagcccac gccatttacc cctgccatgc cgttcgatcc
     8401 gcagatcgcc aacaaggcgc ctcgagtaaa atgcggaatt gagatttccc gtgtgcctca
     8461 atgacgcctg gacgcccctc cgcatcaccc gatgatcgcc tgacccccgg tggccgctct
     8521 gacgacgacg cgcaacgaca tgccgccggc cggcgcagca cctgggccag cgtcgccgtc
     8581 aacgtttgcc tgatcatcat gcaggccacg attggcacgc tggcgcattc gcaggcgctg
     8641 atcgccgatg ccatccattc actgtccgac ctgctctcgg acggcatcgt cctggtggcg
     8701 ggcaagcaca gccacaaggc gcccgacgcc gatcacccct acggccattt acgcttcgaa
     8761 accgccgcga cgctggcgtt gggcgcgatc ctgctggcgg tgggcgccgg catgctctgg
     8821 aacgcgttcg agcatctgca acaaagccca gaatccgatg gccgcgtgag cgcggccgcg
     8881 ctggtggcgg cagtgatcac gctctgtgcc aaggaagggc tgttccgcta catgctgggg
     8941 gtggcgaagc gggcgcgctc gtccatgctg gttgcgaacg cctggcacgc ccgctccgac
     9001 gcggcctcat cgttggtggt ggcggtgggc atcatcgcca gcctggccgg ctttcccatg
     9061 gccgacccgc tggcggcgct gatcgtcggg gttatcgtgg gccgcatggg gtggaagttc
     9121 tttctttccg ccttcaacga cctcaccgac cgggccgtgg acgaggaaac cgaggcccgc
     9181 atccgcgcgc acatactggc caccgacggc gtgctgggca tccacggcct gaaaacccgg
     9241 aagctgggcg acatgatctg ggtcgaggtc gacctggaaa tggacgccga cctgaccatc
     9301 gcgcaaggcc atgccatcgc cgaggcggcg cgcgccaacg tgttgcgcca cgaaccggtg
     9361 ctcgacgtca tgacgcactt cgatccggtg cgggtcactt ccgatgggca ccaaacgcgt
     9421 cgttgaaccc gtatagctgc gccctgtatt tttcccggcc ctgccgggcc gccaggcgca
     9481 gcggcgccat taccctggcc tgtcatcccg aggctggctt gtcatcctga ggctggcttg
     9541 tcatcctggg gccgctgtgg ggccgacgcc cggcctccgc gtcgagcaca gccaggatgg
     9601 gccggaccat gccctcgagc agcggtttct cgggccgcgt gcgagccatg acacgcacgc
     9661 caatgtgagc agcgagcaag gcacgcccga agtccgtggc agactgggac cgggtgatcg
     9721 aaccgtcgcg ctgacccgcc cggacacacc ggcggaaaaa agcctctatc tgcgccagga
     9781 tttccgtgac gatacgccgg aattccggat cgtggggtgc cacttccagc gccgaattga
     9841 tgagcatgca ccccttgcgc cgcgggtcat ccagcgaacg ccggatgatt tccatgaaga
     9901 aggcccggat ggcctcgagc ggcggcaggg tgccttcgag ccggcgcacg cgatccgcga
     9961 aactggcgtc gatatagtga tcgagcgcct tgcggtacaa ggcccgcttg ccaccgaacg
    10021 cgttgtacag gctcgcgccg gtcagcccca tgcgaccggc gagctcccgc gtcgaggtcg
    10081 cttcatagcc atgttcccag aagcacgcca ctgcggcatc caggacctcg gcctcatcaa
    10141 attctcgtgg tcggcccatc gcaactcacc tgtcatgaac gacaacatta tgcatcggga
    10201 tgggcgccgc tcgaggaccc aatcatttcc gaaccttcga accatcgcca gaaggcggct
    10261 caaaatttct gtttgacaat tttagatcga atgatataaa actaatgccg gtaccgcaac
    10321 tgatgccggc accgcaagca gccaggcacc tcatcaacaa ggccggccct ctatcggaga
    10381 ctcaagcaat gatccgtttc tattaccatc cgacacccaa tccagccaag gttgccttgt
    10441 tcctggaaga aacaggcctc gcctatgaag tcgtgccggt cgacaccagc aaggggcagc
    10501 agcacgatcc cgcattccgc aaaatcaacc cgaacggcaa ggtgcccgcg atcgtggata
    10561 tggaaggccc tggcggccag cccgccacgg tgttcgattc gacggcaatc ctgctctatc
    10621 tcgccgagaa ggccggtcga ctcctgggcc agcctcacga tcgccccgaa ttgttgtcgt
    10681 ggctgctctt tctggggtcg ggcctcggtc ccttctcggg tcaggccgtg catttccagt
    10741 acgcggcgcc ccccggactg gactatgccg tcaaccgcta tcgccgcgag gccgaacgtc
    10801 actatcacgt gctcgacgac cacctcgccg gccgcgaatt catcgtcggc aatgaataca
    10861 ccatcgccga catttccgct tggggctggc tagatcgagc atctcgtgta atgaaaggca
    10921 cagaagaccc actgggtgcc tatcctgaca tcaagcgctg gttccaggcc atcgacgccc
    10981 ggcccgccgt cgcgcgggcg cggcaggtcg gggcggggct tgcgttcaag acagtcaacg
    11041 acgaggaaac gcggcgggcg ctatttccct cgaactaccc tgcggcgggc tagccgccgc
    11101 gtccggccaa tccggaacaa ccggccagaa cacagacggc ctccggttgc gaagaatcac
    11161 tcctttccac ttctgcagga gaaaacccca tgtcagccac gaaagcgatc gtcttcgatc
    11221 tgtacggcac gctctacgat gtccactccg tcatcgagca gtgtgactgc gcctatccgg
    11281 gcaagggcgc ggaaatcagc ctcttgtggc ggcagaaaca gctcgaatac acctggctgc
    11341 gcagcctcat gggccagcgg ttgtacatcc cgtttgaaca agcaacccgt gatgcgctgg
    11401 tgtacacctg cgggcatctc ggagtcgacc ttgacgacac cacgtgcgcg gcgctctgcg
    11461 atgcatacct gaagctgtcc ccctaccccg aggtgccggc cgcgctggca cggctgcagt
    11521 cgatggggct gccgctcgcg atcctctcca atgggtccgc gttctcgatc aacagcgtcg
    11581 tgaaccattc cgggctgcag gcgcgcttca cgcacctgct cagtgtcgac ccggcccgca
    11641 cgttcaagcc cgacccacgc gtctacacac tggcgtgcga ggcattctcg ctgcctgcgg
    11701 accagatcct cttcgtctcg tccaatgcct gggatgcctc gggcgcctac cacttcggct
    11761 tcgacgtctg ctgggtcaac cgacgcggcg cggtgttcga cgaactcggc gccgcaccgc
    11821 gggccattgt gaagggcatc gacgagatcc cggctttggt cggctgaatc cgtgtcccat
    11881 ggtgcatcat gcgccgccga gagccgccag acccgattac ctggcgcggg gcagcccgcc
    11941 tcgctccagt tgccccaggc tcgccgccaa cttgaactgc cctgttttcc attgcgtcag
    12001 cgccagaacg cgctgccgtt cggcattggt caggtcactc tgtgcccgca acagctccag
    12061 caacgacccg acgccggcct tgtagcgccc gaacgccacc ttgaccgatt gtcgggcact
    12121 gtgcagcaga tcattcgcgg tctccaacgt ggcgagccct gatgtgaatt cctcataact
    12181 cttccacaca tccagggaca ccgtgcgcct tgcctgggcc agatccgcct tcctggcatc
    12241 gacctccgca tcagccttgc tgatctggta gtgactgcca acgtccacaa ttggaatcgt
    12301 gagctgcagg ccaatgctgc gattgttgat gacctgtcgg ctcgtcactt gggtgatcgg
    12361 cgtgtcgctg cggtccatgg aagccatcag ggacagcctg ggcagactct ggctgcgggc
    12421 cgaccgggcc tcggctcgag ccgcttcaag cttcgcccgg gcggcaagaa cgctggggtt
    12481 ggtgtgcatc gcctccccca ccagcgtgtc aacggtcttc cggtaatcat cctgatgcac
    12541 cccatcgtca tcaacagatg gcaaatgcag agagacaccc ggagccagcc ccatttccac
    12601 cgccagggcc cccatggcct tgtccagatc actttgagcc tgcatgcgtt ccaggacggc
    12661 ctgggaatac gcggtcttcg cctgcagctt gtcggccagc gtcgcggcgc cggccttgaa
    12721 ttttgcctgc gcgatcacaa agctttcatg tgcaatccgc tctgtctctc tgtcagcccg
    12781 caacagcgtc aggcgggtct gcgcatcata gtagacacgc accgtgtcca ggaaaacggt
    12841 ctgcagcgtt tcattgcgcg aggcgatcgc cgcattcagc aactggcgac tgtgctccag
    12901 attggccgcg cgcaggccga agtcgtacaa cacccaactg agtgtcaggc cgacgtcggt
    12961 gcttcgtgta cgcagcgacg actcgagccc gggcaggttg gtataagtga cgcgctgatc
    13021 aacgcgatca acgctggcgt tggcgctaag cgtgggccaa taggccgcgc gatcgacatt
    13081 cagttgcgcc tgctgtgttt gtatgtaggc ccacgcctgg cgcgtacgag ggtcgctgca
    13141 cagcgcacgc ccaaccgcat cgagcaatga tacgggcata cggccaggga tcccctgggc
    13201 gcagaaccgg cttccagtgc ctggactgcg ccccgcctcc tgtcgccctg cgaggagcgt
    13261 atccgggggc tgcgccagat ctttggcgtg gacgcaactg ttggccagca gagccagccc
    13321 cacggccgcc agcgtctgtc cccagcgcac cagccccgtg gccccgcatg acagagccag
    13381 gcggaagatc gggaccgtca tgacgcatcc gcgacggcgg cggcaccgcc gcgcgccggg
    13441 accgccggcc cgtcgtacac gatgcgaccg cccaccagga cgatgcagcg tccggcgctg
    13501 gcaaccgtct cgggccggtg cgccacgatc acccgcgtca tctgcagagt cagcacggcg
    13561 gcgttgacct ggcgttcgcg ttcgatatcc agatggctgg tcgcttcatc caagaacagg
    13621 attcgcggct gcttgtacaa agcgcgcgcc agcaagacgc gctgcttctg cccgcccgac
    13681 agcaccgtcc ccatgtcacc gacgagcgtg ttgtaaccca tgggcatggc gacgatgtca
    13741 tcatggatcg ccgccaactc cgcgctgcgc cgcatgcggg cctggtcggg ttgcacatcg
    13801 aagaaacaga tgttctccgc gattgatccg gcgaacagca catcatcctg cagcactgtg
    13861 cccacgaggc gtcgcaatgt accggatccc agccgctgcg ttggcttccc gtccaccagg
    13921 atttcgcctt ccgtgggcgg caacaggccc agcaacaatg cgatcaacgt cgacttgccg
    13981 ctaccagagg ggccggtgat cgccactgac tctcccgcgg ctatcctgaa gctgacacca
    14041 tccagcacgt agggctcttg gtcgccatag cggaaccgca ggtttcttgc ctctatggac
    14101 ggctgagccg atacatcttc ctcaccaacc tccccaccat cctcactctc agagggatga
    14161 aaaacaatgt cggccaaccg ctctccctgc aattgcaaca tccgaacgtc cacccatttg
    14221 tccaacagac cactgacccg ggtatcaaac tgccctttat aggcattgaa ggcaatcagg
    14281 gcgccgaccg tgaaggcgcc gtccaggacg aaccgcgcgc caagccagat gatcaggatg
    14341 ttttcaatgc cgaacagcac cccgttcagt gtcttgtaca gcagcgtcag cttctgggtg
    14401 gtcaggtcgg cgttgatctg gttcaccagc agggacaacc aagtggaacg acgttgttcc
    14461 tggtgctgga acaacttgat ggtcctggct ccgcgtatgg attccagcag gtgcgtctgt
    14521 tgtttcgcgg catgcacgat gcgctcctcg gtggcattgc gcagcggcgc ataccaggcc
    14581 catcgactca acgcataaag cgccatcgcc cccacggcga tgccggcaag agcgggacta
    14641 tacaaaaaca tcaacaatag cgtgacgacc gtcatgaccc cgtcgagcag cgcctcgacg
    14701 aacgatgtgg tcagcgtccg ctggatggcg tccaccgatc cgacacgcga caccacatca
    14761 cccagatgac gtttctcgaa atactccata ggcaagcgta aaagatgccc gaaaacattg
    14821 gcgcgccatt gcacattcag tgtcgtgcca acgtacatca gcatccagcc gcgcgccgcc
    14881 gacgcggcct gctggaacag cagcagcagt ccgaaaccca gcgccagtgt cgtcaacagg
    14941 ctgcggtcaa ccgacaccag cacatggtca atcacccatt gcaggaagaa cggattgacg
    15001 acattgaaga cttccagcgt aagcgccagc aatatgatct gggtgaacga gcgcttcagg
    15061 ccggtgacac gccccagcag ttgcctcaag cgtacggggc gccgggcctg agcttcctgg
    15121 aaaccggcat ccggccacaa ctccagggcg atacccgtga acgaccgcga cacctcttcc
    15181 atcgaatact tgcgcacgcc tgtggccggg tcgtgaacga caacaaagtt ccgccccacc
    15241 tcgcgcaaca caacaaagtg attcaaatcc caatgcagga tgcacggaag gcgcagactc
    15301 gaaagcccat caagatccag tttcaaaggc cgcgtggcca gcttcagttg cgcggcgata
    15361 cgcaacagca tggcaagcgt cacgcccttc aatgaggtcg ggaaacgaga acgcagcgct
    15421 gccagatcca catgaaatcc gtgaaatccg gcgaccatcc ccagacaagc caggccacat
    15481 tccgccgcct ccgtctgcaa cagcaacggc acccgccgcc tcaacccgaa tgagagatgt
    15541 tcaagcatgg atcaaaaccc atccgacaaa ctgtaaagag gcttcaacgc ccactgatac
    15601 agccgccgct tctgcaacat gatgtccgcc tcaagcgcca taccgggcag cagggccaac
    15661 gcctggcccc gcgccgagtc agtttgtgac gcgagccgaa cccgtatcaa aaaaagcgat
    15721 tcagtgtccg actgcccgct gacggcacca ccgcgcaact cactggctga tagggcagtg
    15781 tgagccactg aaagaaccgt gcccggctgt tgcccatagg tttgataggg gtacgcctga
    15841 tagcggatct gaactcggtc tcccggtcgc acgaacccca ccgccttgct gggggcatac
    15901 aacagcgcca caagcttcga cccggccgga acgatcgtcg ccagcggccg gtcgccgcct
    15961 atggcctggc cgggatcagc caaaaccgcg gccaccgagc ccgtgaccgg cgcggtaatc
    16021 agttcgcgac gcttggcttc actttcgctc agggcctctg cggcattggt gaggtcacgc
    16081 tcgaacctgg cggcctcgtt ctgttgcttc aacgcgagct ccgccaaatc agcgcgctgc
    16141 gccgacaact gctcactcat acggatccgg tcgcgctgca atgactgaag ttgattgccc
    16201 tggtccagcg cgtcctgctg tcgggcttcc agctgctccc tcgacacata gccctgcgcc
    16261 accagcttgc gatatcgatc aaccgattgt cgcgcaagcg ccagccgcgc acgctggcct
    16321 tttatctgag catccagggt ttgcaattcc gccgccaggg cggaaatttt tgtcaccaga
    16381 ctggttcgct cgcttttatg caactggcgg gttcgctgct gttccgcgcg caagctcgct
    16441 tcgcgtcgcc cgacctgggc actgatggct gcctgagtcg agccaagctg gctgcgcgtg
    16501 tcggtggaga cgacatacag cacatcaccc gccttcactt tctggccctt gcgcacacga
    16561 cgctcaacca cgactcccgc acgcggcgca cgcaacgtga tgacgccgct atctggcact
    16621 aattgaccac taaccgtttc tcgcgcggtg taggtgccca gcaccaggaa cagcaagacg
    16681 gcgaccgccg ccgccacagc aaccgcaacc acgatgctga acgaaaccgg gcggaccagg
    16741 acgatatcgc ccagccacgg cgtttgccgg gcctccagag cttctttacg gaataacgac
    16801 acgcttctta ttctgttccg tggacgcttt aaggttcacg cttgccgagc catgatatgc
    16861 acccgcacgg ggagccacac gcggcaaaga gagaagaaac gacatgggca agccacgcat
    16921 gcccatgtcg tagacgcaac tcagccagaa aggctggttt gctggctcga gctattggtt
    16981 agctcaaaag cggcactgaa ggtactcgaa cgggccaccg gtatagagcg agcagtagct
    17041 gaacgcgccg tggtaatccc agggggccat gccaccgctg acttgttcga tttcagatac
    17101 tgtcagctcc tgaaccaggc tttctttgtt gtcttggtat tgagtcattg aacttccttc
    17161 actttgttgc tggtgatacc tgccgcacca gcttcggcta ttgactgcgt gtccagcagc
    17221 tcgtggccgc agggcgaagc caaatcgtta ccaggaaccc cagcccgcca agggcttttt
    17281 ccggtgaacc tagattcgac agaatgatac aagcgtatat acgatgtatt tgtgacaaat
    17341 agatttttaa tttttcaaaa tgaccggtcg agcatgagtg catgccttag cgcggcgctt
    17401 tgcaggggcc acgccatgca gcgcggtgat atgcgtggat gggtcggcgt ccggcgtgat
    17461 ccctgtcacg tgtatggcgc tggacaccaa gtccggaaaa tcgtgacgct tacttgcttg
    17521 ttttcaacgt cacaacaccg agtccagcag cagcttcacg ttgagcgcgc aaatcagcac
    17581 cgcgaccgcc caggccagca tcgtggcggc cttgccgatg gcgaaagccc ccatcatttt
    17641 cttgtcggac acaaaacgca cgagcggaat gacggcgaac ggtagctgca tggacagcac
    17701 cacctggctg agcaccaaca gccttgccgt cccgccctcg ccgtacagac tcgtgatgat
    17761 cacgaccggc acgatggcca gcccgcgtgt caacaagcgc tgcgcccagc cggggagacg
    17821 cagacccaga aagccttcca tcacgacctg cccggccagg gtggccgtga cggtcgaatt
    17881 gatgccggac gccaacaagg ccaccgcgaa cacccaggac gccagtccca cccccagcat
    17941 gggcgacagc aagtgatacg cctggctgat ctcttccaca tcggtgcgcc cggccgcgtg
    18001 gaaaaccgcg gcggcggtga tgaggatggc cgcgttgacg aacaacgcca gcagcagcgc
    18061 aatggtgctg tcggccaccg cccagcgcag gccttcgcgt cgccccgctt ccgtgcgcgg
    18121 atagtcacgc gtctggacaa tggccgaatg cagatagaga ttgtgcggca tgacggtcgc
    18181 gccaatgatg ccgatagcca agtaaagcgc ggccggatcg gtcacgatgc gcgcctgcgg
    18241 catgaacccc ttgaccaagc ccgccacatc ggggcgggcc aggacgatct ggatgccgaa
    18301 gcacaccgcg atgatcaaca gcaatgtcat cacgaatgcc tcgagtcggc gaaaaccccg
    18361 ctgcatcagc agcagcacca cgaatacgtc cagcgcggtc agtaccgcgc ccaacaccag
    18421 cgggatgccg aacaacaggt tcaacgcgat ggcggtaccg atgacttcgg ccaggtcgca
    18481 cgcgatgatg gccgcttcgc acgccaccca caacagcaag ctggcggatg gccgataacg
    18541 ggcacggcat gcctgggcca gatccagccc agtggcgatg cccagccgtg cggcgaacgc
    18601 ctgcagcagc atcgccatca gactggaaag cagcacgaca gacagcagca aatagccgaa
    18661 gcgcgagcca cccgcgaggt cggtggccca gtttccggga tccatgtaac ccaccgacac
    18721 caggtagccc ggcccggcaa aggccagcag atggcgccac cagccaccca atgcgggcac
    18781 cgccacgctg gcattcatct ggtcgaggct gggcgaaaca ggcacggcgc ccccctgatc
    18841 catacgatgg aggtgagccc gcagcgtttt catgacatca ctcgcgacaa ctaccgttct
    18901 caacggagaa ctcttctacg ttttatagta tgtgctatat tttatagcac agaataactc
    18961 gaggttgcgg caccgcacga cacgcagcag cgcccatgac caaaacccga acacccacaa
    19021 tgtttcagcg cacactggtg ccgccagagg tccacgccga aggctttgtg caagcacgcc
    19081 atgcgcaccg gcaggagttg gccgaagatt acgttgaact gatcgacgac ctgatccacg
    19141 atggtggcga agcgcgacag gtcgacatcg ccgcccggct gggcgtggca caacccaccg
    19201 tcgcccgaat gctgaagcgg ctcgacagcc aggggttggt cagcttgcgg cgctaccgcg
    19261 gcattgcgct caccgaggcc ggccaggcgc tcgcggaggc cagccgcaag cgccatcagc
    19321 tatgcgaatc actgctactc gccctgggcg tcggcgcaga cgccgcgcga cgcgacgcgg
    19381 aaggcatcga gcaccatgtg agcgaagaaa cgctgaaggc cttcagccga ttcctggcgc
    19441 gggaacgggg gaaaaccacg cgcgcgggcc aggcatgagc acgtcctgag caccacaggc
    19501 ttccgtgggc tattgaaatg gaacaagaga agacaaccca cttatgttgg ccttgacccc
    19561 agccagccta tcggacacgc ccagcggtgt cttcccgtag cgccttctgc aacagttgat
    19621 ccagggcgtt ggcaaacgcg cgcctgtccg cttggctgaa agccgccggc ccgccggtat
    19681 cgacgccggc gctacgcagt tcttccatca tgtcgcgcat ggacaggcgc tcgccgatgc
    19741 tgtcggccgt gtaacgctcg ccgcgcgggc tgagcaccaa cgcacccttg cgcagagcct
    19801 cgctcgccag ggggatatcc gcggtgatga ccagatcgcc ggcggcggcg tgttcaacga
    19861 tatagtcgtc cgccacatcg aatccgcgcg gcacctggac tgcccggatc aaaggagatg
    19921 gcggcgtgcg cagcatttga ttggccacca gggtgagcgg ccgctgcgac cgctgcgccg
    19981 cccggtacaa gatgtccttg atgacggcgg ggcaggcgtc ggcatcgacc caaatattca
    20041 tggacccggg cctgttcaca ctaaatggcg cccttcagcg cccccgccag cagcgtgttc
    20101 accagttcgt tgacactgac gccatccgcc tgcgccttgg cgttcaaggc cgaaatcaat
    20161 ggctggcgca gcttaacggg gaaggacacc aggccagcgg cttggtccag gcgacgctgc
    20221 tcgcggcgat ccacaacggg ctcggcgccg gcaaagcggc ccggcgggac ggcggcgttg
    20281 agcttgccca tgagtttcag ggctttctgc ttttcgagat cggttttttt catgaagggc
    20341 tcgcgttgtg gtctgccaat ggcagactcg tcaggcagca tcttagagga tatcgaagat
    20401 gaaaaggggt ctgccctgaa ggtctgctcg ccaagcgaca gccaggagtg aatcgtgagc
    20461 ggtcagccaa gcagagggcg gcaggcaccg ccgaaaaaac aaaaacccaa acagaagtct
    20521 gggttttcag atactggagc gggtgaaggg aatcgaaccc tcgtcttaag cttgggaagc
    20581 tcgcgttcta ccattgaacc acacccgccc tgcgcgtgcg ccatacaggc acgattgaag
    20641 ctgcgtatta tagcgtcttg cgcggaacgt ggttggcggc cggcgaaacc acgctccgcc
    20701 tggaaattcg tcgattgccc catgaaagaa ccgacggcaa tcactacaat gacatccatg
    20761 acttcaacga ccgaccctac ctcgaacggc ccggcaaccc cggccgcctc cgcagcaccg
    20821 gccgcggccc attccgacca tggccacata cgcagtttcg tgcatcggcg cagccacatc
    20881 acgcccgccc agaaagaggc gctggatcgc ctgatgcccc aatgggccat cccctaccag
    20941 ccgcggccgc tggatttcgc caccgccttc gggcgcgcgg cgccggtcgt cctggaaatc
    21001 ggcttcggca tgggcgagac caccgaacag atcgccacgg cgcgccctgg cgacaacttc
    21061 ctgggggtgg aggtattcaa tgccggcgtc ggcgggttgc tcaagcgcat cgaatcatcg
    21121 ggcctgacca atatccgcat catccagcac gatgccgtcg aggtgctgcg cgacatgatc
    21181 cccgtcgatt cgctcgccgg cgtgcacatt tatttccctg acccctggcc caaggcgcgc
    21241 catcacaagc ggcggctgat ccagcccccg ctggtggccc tgctggccag ccgtatccag
    21301 ccaggtggct acctgcactg cgcgaccgac tgggaaaact acgccgaaca aatgctggcg
    21361 gtgctcagtg gcgagccgct gctgcgcaac acctgcgatg gctatgcgcc gcggccggag
    21421 taccgcccgc agaccaagtt cgagacccgg ggcctgcgac tgggccattc cgtgcgcgac
    21481 ctgattttca cgcgcgtggc acccacggcg tcctagcggg gcacaccgcc gccacgaaac
    21541 gcctcatgcg aacctcgctc tcagccatac actgttagaa aacgccccac gcaaggagca
    21601 cgccatgtac cctgaaatcg aaccctaccg ccacggcatg ctggacactg gcgacggcca
    21661 ccaggtctat tgggaattat gcggcaaccc ggcgggcaaa cccgccatgt ttctgcatgg
    21721 cggcccgggg ggcggttgct cggtcaacca ccgtcgccag ttcaatccgg aacgctacaa
    21781 cgtgctgctg ttcgaccaac gcggctgcgg acgctcgacc ccgcacgcca gcctcgagaa
    21841 caacaccacc tgggatctgg tcgccgacat ggagcgcctg cgcaccgagg tgctggacgc
    21901 cgagcaaagc ctgattttcg gggggtcgtg gggctccacc ctggcgctgg cctacgccga
    21961 aacgcacccg cagcgggtga gcgagctgat cgtgcgcggc atcttcaccc tcaggcgcga
    22021 tgaactgctg tggttctacc aggaaggcgc atcgtggctg ttcccggacc tctgggaaca
    22081 ctacctggcc cccattccgg tcgacgaacg tggcgacatg atcagcgcct accgcaggca
    22141 gctgatttcc aacgaccgtc agacgcaact acgcgccgcc catgcgtgga cgcaatggga
    22201 aagccgcacg atcacgctgc tgcccagcat ggcgcaccag caatcccacg cggaggacga
    22261 ttccgccctg gccttcgccc gcatcgagaa ccactatgtc gcgcatggcg ggttcatgga
    22321 agacgggcag ctgctgcgca acgcccacaa gctgcgggac atccccggcg tgatcgtgca
    22381 ggggcgctac gacgtgtgca caccggcgcg caccgcctgg gacctgcaca aggcttggcc
    22441 gcaggccgag ttccacctga tcgacgacgc cggccacgcc ttcgacgaac ccggcatcct
    22501 gaccgaactg ctggccgcca ccgacaaatt cagccgttag cgttcgctga tcgcacggca
    22561 agcgcgggcg ccgaagttgg ctaatgcatt ccgccaaagc cgccgcggcc cattcctttc
    22621 aggttgccca tggcgcgcat catcttggcc atgccgccct tcttcatctg cttcatcatg
    22681 ccctgcatct gctcgaactg agtcagcagg cgattgactt cctgcaccgg cacgccggcg
    22741 cccgccgcga tacggcgctt ccgtgacgct ttgagcaacg cgggtttcgc gcgctcttgc
    22801 gccgtcatgg aattgagtat gccttcggtg cggcgcaact gcttgtcagc ctggccgctt
    22861 tgcaactggc cggcggcctg ggcgaattgg gacggcagtt tttccagcaa cgaccccatg
    22921 tcgcccattt tcttgacctg ctgaagctgg tcgcggaaat cgttcaggtc gaagcggccg
    22981 ctggacttca gcttggtggc cagcttctgc gcttcggcga tgtcgatgtt cttctgcgcc
    23041 tgctccacca gcgacacgat gtcgcccatg cccagcacgc gctgcgccat gcgttccggg
    23101 tagaaaggct cgagcccgtc cagtttttcc gacacgccca cgaacttcag cggtttgccg
    23161 gtgacatgcc gcaccgacag cgccgcgccg ccgcgcgcgt cgccatccag cttggtgagc
    23221 accaccccgg tcagcggcag cgcgtcggcg aacgccttgg cgacgttgac cgcgtcctgg
    23281 ccctgcatgg cgtcgaccac gaacagggtc tcgatgggat gcagcaagtc gtacagggcg
    23341 cggatctcgc gcatcatggc ctcgtcgatc cccagacggc cggccgtgtc gacgatgagc
    23401 acgtcgaaat gatggcgccg tgcgtgatct agcgcggtgc gggcgatgtc ttcgggcttc
    23461 tgggatccgt cggacgggaa gaactccacg ccggcctgct cggccacagt cttcaactgc
    23521 tcgatggcgg cggggcggta gacgtcggcg ctggtcacca gcaccttctt cttgcccgtc
    23581 ttgcggccat gctgcacgtg gccgccttcg gccagccatt tcgccagctt gccggtggtg
    23641 gtggtcttgc ccgcgccctg caggccggcc atcagcacga tggccggcgg ctgcatggcc
    23701 agggaaagct cgcccgcttc cgggccgaga tcttcgccca tgagggcggt gagctgcttg
    23761 tgcaccacgc cgaccagcgc ctggccgggg ttcaggctgc ccacgacctc ctggcccatg
    23821 gcgctttcgc gcacgcgctc gacgaaatcg cgcaccacgg gcaacgaaac gtcggcctcg
    23881 agcagggcca tgcgcacctc gcgcagcatg tcgcgggtgt tggcctcggt cagccgggcc
    23941 tgcccgcgca tcgtcttcac gacatgcgaa aggcgttgag tcagattatc gagcatgaca
    24001 gggattcgct tacactaatg aatcgtggat gctccggggc gcgggctaaa ataagccttc
    24061 ggcccgcaaa gccgaaccaa ccagaattca agggtttatg tccgcaggca ttgtatttca
    24121 cttgctggcc gcgatcgcct atgcgatttt agccatttcc ttgtggcgcc cgctgtcgcg
    24181 gggcggcacc gaggcaaaag ccgggaaagt cgggcgcatc tgcctgctca gcgccatcat
    24241 ccttcacggc atcgccttgc agcaatcgct gctgccgggc cacagcgtgc acctgggcct
    24301 ggcgccggcc gtatcggccg ccctgtggct gggcatgatc gtgttctggc tggaaagcct
    24361 gctggtgcgc atcgacccgc tgctgctcat tttactgcca gtggtggccg tggtctgcct
    24421 gctcagcgcc atcttcccgg aaacccacgc catcgcgcat gccggcagcg acttgctgcg
    24481 gctgcacctg ctcatcgcct tgagcgccta cggtttgatc accgtggcgg ccctgcacgc
    24541 catcctcatg acggcgctcg accgccacct gcaccggccc gtcacccagg ccgcccaggg
    24601 caattccctg atgcgcgcgc tggattccat gccgccgctg ctggtgcagg aacacctgct
    24661 cttccgcctg atctggatag gcttcgcggt gcttaccctg gccgtcattt cgggcgcgat
    24721 ggtgtccatg cggctgatcg gcgtcccgtt tcccttggac cacaagacgg tcttcaccct
    24781 gctgtcgtgg ttcaccttcg gcgggctgct ggccgggcgc tacatccgcg gctggcgcgg
    24841 ccgcatcgcg ctgcgctgga ctctagtcgg cttcgtgttc ctgctgctgg cctacaccgg
    24901 cagccgtttc gtgcttgaag tgctcctgca tcggggctga aatggaaaaa ctcttcagtt
    24961 gggtcctgct catcgtcgtg ggcctggtca tcgcgcgcat cttgtcgcgc cagaacgccg
    25021 cccgccagca catggccacg cgccaggcgc cgcgacgcgg cgccccgcca tccatcgcgc
    25081 ccgaggccat ggtgcgctgc gcgcactgcg gcatccatct gccgcgatcc gaagccgtga
    25141 tggtgcaggg caagacctgg tgcagcgaag agcacgccag gctggggccg cgccaatgag
    25201 ccgcctggcg ctgggccgaa acggctggct cgagccagcc gccggcgtgc gcctggcgcc
    25261 ttcccccaac ttcgatgcgc gccccgcggg ggtcgagccc atactgctgg tggtg
//
LOCUS       DASTNK010000076         7027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_794154, whole genome shotgun sequence.
ACCESSION   DASTNK010000076 DASTNK010000000
VERSION     DASTNK010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794154"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VFR20_05805"
     CDS             complement(<1..513)
                     /locus_tag="VFR20_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HEU0230064.1"
                     /translation="MADGTVFKGVSIGAKGYSVAEIVFNTAMTGYQEILTDPSYSGQI
                     VTLTYPHIGNTGINDEDVESSRIHAAGLVVRDCSLRVSNFRATQSLPEYLESQGVVAI
                     AGIDTRKLTRILREKGAQGACILVGEDIDQAVRLAREFSGMSGQDLARVVSVDKSFEW
                     HEGTWRLGEGY"
     gene            complement(957..1751)
                     /locus_tag="VFR20_05810"
     CDS             complement(957..1751)
                     /locus_tag="VFR20_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0230065.1"
                     /translation="MSNTRKHRSYMMRFLQVLLLVILIAAWHFASRNVNFAFFFGKPA
                     QVVQVIWAWFVTDGDIYHHLWITLTETVLAFVIGTVTGLACGLWLGLSRGASALLEPY
                     IKALNSMPRVILAPIFGMWFGLGIWSKVALAVTLVFFIVFFNVYQGVREVSTTLLDNA
                     RMLGASRHQLLRHVYIPSATSWVFSSLHTSVGLAFVGAVVGEYLGSASGVGYLILQAE
                     GTLDVNTVFAGIVVLTIFALILDGIVGLVEEHFLVWKPKAGETEKI"
     gene            complement(1755..2564)
                     /locus_tag="VFR20_05815"
     CDS             complement(1755..2564)
                     /locus_tag="VFR20_05815"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0230066.1"
                     /translation="MPDAALLLDHITCTFKSNRGDNASYTAVKDAHIAVAPGEFVSVV
                     GPTGCGKSTLLNVGAGLLAPSSGQIKVFGKPLKGVNQRAGYMFQGEALLPWRNALDNV
                     TAGLEFAKVPKADAVSRGREWLKRVGLGGFENRYPHQMSGGMRKRVMLAQTLIRNPDI
                     ILMDEPFSALDIQTRQLMENEVLDLWMAQRKAVLFITHDLDEAIAMSDRVVVLSAGPA
                     THPIGEFIIDIPRPRDVTEVRGNPRFIELHAAIWDVLREEVLKGYAQQKRA"
     gene            complement(2557..3597)
                     /locus_tag="VFR20_05820"
     CDS             complement(2557..3597)
                     /locus_tag="VFR20_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0230067.1"
                     /translation="MSISRRDLLKLAGAAGAATVVPGISRAASAEKKDVIIAVGGQAL
                     IYYLPLSIARDLHYFKDEGLNVKVMDFAGGSKALQAVVGGSADVVSGAFEHTIFLQVK
                     GQMYRTFAQQGRAPMMVLGVSTKTMPNYKSPADLKGKKIGVTAPGSSTNMMVSFFLAK
                     HGLKANDVSYIGVGAGQGAITALRTGQIDAICNVDPVISTLVHAKELKVIADTRTLKD
                     TRDIFGGDMPAGCLYASQKFIDENPKTTQLLANAIVRADRWIQENSIDKIAATVPKQY
                     LLGDPDLYKLALKANMQALSPDGLVPAGGPETALKALAAYVPHFQADKIDISKVWTNA
                     YALKADKKYPHA"
     gene            complement(3743..4351)
                     /gene="recR"
                     /locus_tag="VFR20_05825"
     CDS             complement(3743..4351)
                     /gene="recR"
                     /locus_tag="VFR20_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEU0230068.1"
                     /translation="MEPALPEPEPLTALIQALRRLPGVGMRSARRMAYHLMQHDLAGA
                     DQLARALSDAVRTLGHCPRCNSFTEGDVCATCANPKRDPSLLCVVETPSDQNMIESSH
                     GYRGLYYVLMGRLAPLEGVGPEDLHFDRVIRRATDGVVREVILATNFTAEGETTAHYL
                     SESLRSRGLTVSRLARGVPAGSELEYVDAGTIAWALMERRST"
     gene            complement(4351..4677)
                     /locus_tag="VFR20_05830"
     CDS             complement(4351..4677)
                     /locus_tag="VFR20_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU0230069.1"
                     /translation="MMKGQIAGLMRQAQQMQENMKKAQDALAEIVVDGASGGGLVKVS
                     MTCRHDVRRVAIDPSLLGDDKDMLEDLVAAAFNDALRKAETTSQEKMAAVTAGMPLPP
                     GMKLPF"
     gene            complement(4786..6942)
                     /locus_tag="VFR20_05835"
     CDS             complement(4786..6942)
                     /locus_tag="VFR20_05835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU0230070.1"
                     /translation="MTYLVLARKWRPRSFDTLIGQEHVVRALSHALSTGRLHHAWLFT
                     GTRGVGKTTLSRILAKSLNCEAGITATPCGTCRACTEIDEGRFVDYLELDAASNRGVD
                     EMTQLLEQAVYAPSVGRFKVYMIDEVHMLTGHAFNAMLKTLEEPPPHVKFILATTDPQ
                     KIPVTVLSRCLQFNLKQMTAPSIVEHLGSVLDQEHIAYEVPALRLIAQAAAGSMRDAL
                     SLTDQAIAYSAENLTADAVRGMLGTIDQRYLARLLRAMSAGCGADVLAVADELATRGL
                     SYSGALADLAVLLSRIAIAQRLPGTIPADDPLAAEIAELAGQLHPDLIQLFYSVVVHS
                     RGELSLAPDEYAGFVMACLRMLALAPANPDPTAAPVPEDARQDTPAATGTGAPVASTT
                     ARASSAVSATAQALSTPSTPPQQASPAVGLVPAGRSSVDEVPAWEELPAESSHDNPGA
                     RIDFAPTDGRGSPPASSRSPESREMPEPSVTAADTGDSEGQVGGSVHPEGQSDSHGLA
                     TVAQPTVPAAVDGGQPSDNAPDDGPPESWYEDAGLVPDDAGFTSPDDSEGFETLVPDV
                     KRAMGAMPGRSPVEQASPSLRAMSPDAWPALAASLPLTGLAAELARQSEWLEADDHRV
                     ALRVAVRSLVDTHGRARLRTVLSEYFGTAVQLDIECGATGDATAHAVAQARSEALQRQ
                     AEQDVDADPFVRSLIDEFGGTVIPGSVRAVPDDRAA"
BASE COUNT         1374 a         2187 c         2209 g         1257 t
ORIGIN      
        1 gtagccctcg ccgagccgcc aggtgccctc atgccattcg aatgacttgt ccacggacac
       61 gacccgggcc agatcctggc ccgacatgcc cgagaactcc cgcgcgaggc gcacggcctg
      121 gtcgatgtcc tcacccacca ggatgcacgc gccctgggcg cctttttcac gcagaatgcg
      181 ggtcagcttg cgggtatcga tgcccgcgat ggccaccaca ccctgcgatt cgagatattc
      241 aggcagcgac tgcgtggcgc ggaaattcga cacccgcagc gagcagtcac gcaccaccag
      301 gccggcggcg tggatacggg acgattcgac gtcttcgtca ttgatacccg tgttgccgat
      361 gtgaggatag gtcagcgtga cgatctgacc gctatagctg ggatcggtga ggatctcttg
      421 atagccggtc atggcggtgt tgaacacgat ctcggcgaca ctgtacccct tggcaccgat
      481 cgacacccct ttgaaaaccg taccgtccgc cagggcaagg atcgcaaaag aaggcttctg
      541 agcctcttcg gaaaataatg acggcagcac ggtaaacccc aaatattgta gtgacggaac
      601 gaaaatcaaa ctttcagatt ataccgtgga gaccgtgacc ggccgccgcg cacgcgcgcg
      661 accccgccaa catcaaggct ggcgcgggtt caggattctt tggatcgttt tgaccaagga
      721 ggaagttctg gcccaagaag ttctattgaa cacgcttcgg ggtgaaaggc tgtgccggcc
      781 ggcgcgtcca gcggtcgtgg cgctggccga aacgccggcc ggcacagcct ttcaccccga
      841 agctgcaata gcgacacgca tggcggcaag tgccagcccg ccccatgccc cggtggacag
      901 cgccttcaga taccaacaac gctaaatcca gcacacacct gaaccagcac aaagcgctag
      961 attttttccg tttccccggc cttgggcttc cacaccagga agtgctcttc aaccaaaccg
     1021 acaatgccat cgagaatgag cgcgaaaatc gtcagcacga cgataccggc gaacacggtg
     1081 ttgacgtcca acgtgccttc ggcctgcaga atgagatagc caaccccgct ggccgacccc
     1141 agatattccc ccaccacggc gccaacgaac gccagaccca ccgaggtgtg cagactggag
     1201 aacacccagc tcgtggctga cggaatgtac acatggcgca gcaactgatg gcgactggcc
     1261 cccagcatgc gcgcattgtc gagcagcgtg gtgctgactt cgcgcacacc ctggtacaca
     1321 ttgaagaaga cgatgaagaa gaccagcgtg accgccagcg ccaccttgga ccagatgccc
     1381 agaccgaacc acatgccgaa gattggagcg aggatgaccc ggggcatgga gttgagggct
     1441 tttatgtagg gctcgagcag cgcgctggcg ccccgagaca ggcccagcca caggccgcac
     1501 gccagacccg tgacagtgcc gatgacaaag gccagcacgg tttcagtgag cgtgatccac
     1561 agatgatggt agatatcgcc gtcggtcacg aaccaggccc agatgacctg gaccacctgt
     1621 gcgggtttac cgaagaaaaa tgcgaaattg acgttgcgtg aagcaaaatg ccaggcggcg
     1681 atgaggatga caagcagcag cacctgcaaa aagcgcatca tgtaagaacg gtgtttgcgt
     1741 gtgttggaca tggatcaggc ccgcttctgc tgggcatagc ctttcaggac ttcctcgcgc
     1801 aggacgtccc agatggcggc atgcagttcg atgaagcgcg gattgccgcg cacctcggtc
     1861 acatcgcgcg gccgaggaat gtcgatgatg aactcaccga tcgggtgggt ggccggcccg
     1921 gccgacagca ccacaacacg gtcgctcatg gcaatggcct catccaagtc atgcgtgatg
     1981 aacaaaaccg ccttgcgctg cgccatccag agatccaaaa cctcgttttc catgagctgg
     2041 cgggtctgga tgtcaagcgc ggaaaagggc tcgtccatga gaatgatgtc ggggttgcgg
     2101 atcagtgtct gggcgagcat gacacgcttg cgcatgcccc ccgacatctg atgggggtag
     2161 cggttttcga accccccaag cccgacccgt ttgagccatt cccgcccacg gctcaccgcg
     2221 tcggccttgg gaaccttggc gaattccagg ccggccgtga cgttatccag cgcgttgcgc
     2281 cagggcagca aagcctcgcc ctggaacatg taacccgcgc gctggttgac gcccttgagc
     2341 ggcttgccaa acaccttgat ctgtccggag gacggggcca gcagccccgc ccccacgttc
     2401 agcaaggtgg atttgccgca gcccgtgggc ccgaccaccg agacgaactc gcccggagcg
     2461 acggcgatgt gggcatcctt gaccgccgta taggacgcgt tgtcgccccg gttggacttg
     2521 aaggtacagg tgatgtggtc gagcagaagc gcggcgtcag gcatgcggat atttcttgtc
     2581 ggctttcaaa gcgtaggcgt tggtccagac tttggaaatg tcgatcttgt cggcctggaa
     2641 gtgcggcacg tacgccgcca gcgccttgag ggcggtctcc ggcccaccgg cgggcacgag
     2701 cccgtctggc gacaaagcct gcatgttggc cttgagagcc agcttgtaga ggtctggatc
     2761 acccagcaga tattgtttgg gaacggtggc cgcgatcttg tcgatgctgt tttcctggat
     2821 ccagcggtct gcgcgcacga tggcattggc cagcaactgc gtggtcttgg ggttttcgtc
     2881 gatgaacttc tgcgacgcgt acaggcatcc cgccggcata tcgccgccga agatgtctcg
     2941 tgtgtccttg agtgtgcgcg tgtcggcgat gactttcaat tccttggcgt ggaccagggt
     3001 gctgatgacc gggtcgacat tgcagatcgc gtcgatctgg ccggtgcgca acgcggtgat
     3061 ggcgccctga cccgcgccga cgcctatgta agagacatcg ttggccttca ggccatgctt
     3121 ggcaagaaag aaactcacca tcatattcgt ggacgagccc ggcgccgtga cgccgatctt
     3181 cttgcctttg agatcggcgg gactcttgta attcggcatg gtcttggtgg acacacccaa
     3241 caccatcatg ggcgcacgac cctgctgggc aaacgtgcgg tacatctgcc ccttgacctg
     3301 cagaaagata gtgtgttcga acgcgcccga gaccacatcg gcgctgccac ccaccacggc
     3361 ctgcaaggcc ttggagccgc ccgcgaagtc catgaccttg acgttgagcc cttcatcctt
     3421 gaagtagtgc aagtcgcgcg cgatgctgag tggcaggtag taaatgaggg cctggccgcc
     3481 cacagcgata atgacatcct ttttctcggc actggcggct cggctgatgc caggcaccac
     3541 ggttgccgcg cctgcggcgc cagccagttt gagtagatcc cggcgggaaa tagacatgag
     3601 cgacctcctt taaaaaaacc gttcaattat gccatcaacc catccgggta caagcgtatc
     3661 tttcatactg gtattggcaa tgccacctac ggcacagttt gcatttgaac ggtggcatcg
     3721 tcagggctca gtccgacgga cttcaggtgg aacgacgctc catcaacgcc catgcaatgg
     3781 tgcccgcgtc cacgtactcg agctcgctgc cggccggcac cccgcgcgcc aggcggctga
     3841 ccgtcaggcc gcgcgaccgc agcgattccg acagatagtg cgccgtggtc tcgccctcgg
     3901 cggtgaaatt ggtggccaga atcacttccc gcaccacccc gtccgtggcg cgccgaatca
     3961 cgcgatcaaa gtggaggtcc tccggcccga caccctcgag aggcgccaga cggcccatca
     4021 acacatagta caaaccccga taaccatggc tggattcgat catgttctga tcagacggtg
     4081 tctcgaccac gcaaagcagc gaaggatcgc gcttggggtt cgcgcacgtg gcgcagacgt
     4141 caccttcggt gaagctgtta caacgcgggc aatggcccag agtgcggaca gcgtccgaca
     4201 acgcccgtgc cagctgatcg gcccccgcca aatcgtgttg catcaggtga taggccatgc
     4261 ggcgggccga tcgcatcccc acgcccggca gtcgtcgcaa cgcctggata agcgccgtca
     4321 ggggctcggg ctcgggcaga gcgggttcca tcagaacggc aacttcatcc cgggcggcag
     4381 tggcatgcct gccgtgaccg cggccatttt ttcctgcgag gtggtctcgg ccttgcgcaa
     4441 cgcgtcgttg aatgccgcgg ccacgagatc ttcgagcatg tccttgtcat cgcccagcag
     4501 actgggatcg atggcaaccc ggcgcacatc atgacgacag gtcatgctga ccttcaccaa
     4561 gccgccgccc gacgcgccat cgacgacgat ctccgccagg gcgtcctggg cctttttcat
     4621 gttttcctgc atttgctggg cttgtcgcat caaacccgcg atctgacctt tcatcatggt
     4681 gacttcatcc tgactgaaat aagaattgga agtgaataat ggttgaatct gttcatgccg
     4741 ccaggcgcca agaatgaacc ggccgcctat acctggagcg gcgcgctagg ccgccctgtc
     4801 atccggcacg gcccgcaccg atccggggat gaccgtgccg ccgaattcat cgatcagcga
     4861 ccggacgaaa gggtcggcat ccacatcctg ctcggcctgt cgctgcaagg cctcgctgcg
     4921 ggcctgggcg acggcatgcg cggtggcgtc tccggtggcg ccgcactcga tgtcgagctg
     4981 caccgctgtg ccgaaatatt cggacagcac agtgcgcaag cgcgccctgc cgtgcgtgtc
     5041 aaccagggac cgcacggcga cgcgcaacgc gacgcgatgg tcgtcggcct ccagccattc
     5101 gctttggcgc gccagttccg cggccagccc ggtgagcggt agcgaagccg ccagcgccgg
     5161 ccaggcgtcg gggctcatgg cgcgcagact gggcgaggct tgttcgaccg ggctccgtcc
     5221 aggcatggcg cccatcgcgc gtttgacgtc gggcaccagg gtttcgaacc cctcggaatc
     5281 gtcgggcgac gtgaagcccg cgtcatcggg aacgagcccc gcatcctcgt accagctctc
     5341 aggaggcccg tcatctggcg cgttgtccga cggctgaccg ccatcgacgg cggcgggaac
     5401 ggtcggttgc gcgacggttg ccaggccatg gctgtctgat tggccttccg ggtgaacgga
     5461 accgccaacc tgaccctcgg aatcgccagt atcagcagcg gtaacgcttg gttccggcat
     5521 ttcccgggat tccggcgagc ggctcgacgc gggcggtgat ccccgcccgt cagtcggagc
     5581 gaaatcgatc ctggcacctg gattgtcatg tgacgattcc gccggcaatt cttcccaagc
     5641 cggaacctcg tcgacagacg atcgaccagc ggggaccagc cccaccgccg gagaggcctg
     5701 ctgcgggggc gttgacggcg ttgacaaggc ttgcgctgtg gcagacaccg ctgaagatgc
     5761 gcgtgcggtg gttgacgcca cgggcgcgcc cgttccggtt gccgctggcg tgtcttgccg
     5821 cgcgtcttcc ggaacaggcg cggcagtggg atcggggttg gcgggcgcga gcgccagcat
     5881 gcgcagacag gccatgacga agcccgcgta ttcatccggc gcaagcgaca attcgcctcg
     5941 gctgtgcaca acgacggaat agaacaactg gatcagatct gggtggagtt gcccggcaag
     6001 ctcggcgatc tcggccgcga gcgggtcgtc ggccggtatc gtgcccggca accgctgcgc
     6061 gatggcaatg cgcgacagca ggacagccag atcggccagc gcgccggaat aggacaagcc
     6121 gcgcgtggcc agttcgtcgg cgaccgccaa cacgtcggcg ccgcacccgg ctgacatggc
     6181 ccgcagcagg cgcgcgagat agcgttggtc tatggtgccg agcatgccgc gcaccgcgtc
     6241 agcggtgaga ttctcggcgc tgtaggcgat ggcctggtcg gtcagcgaca gggcatcgcg
     6301 catggagccg gcggcggcct gggcgatgag gcgcaacgcg ggcacttcat acgcgatgtg
     6361 ctcctggtcg agcacgctgc ccaggtgttc aacgatggac ggtgccgtca tctgcttgag
     6421 attgaactgc aggcagcgcg acagcacggt gaccggaatc ttctgcgggt cggtcgtggc
     6481 caggatgaac ttgacgtggg gcggcggttc ttcgagcgtc ttgagcatgg cgttgaaggc
     6541 atgcccggtt agcatgtgca cttcgtcgat catgtagacc ttgaaccggc ccacgctggg
     6601 cgcgtacacc gcctgctcga gcaactgcgt catttcgtcg acgccacggt tcgatgccgc
     6661 gtcgagctcg agataatcga cgaaacgtcc ttcgtcgatc tcggtgcatg cgcggcaggt
     6721 gccgcaaggc gtggccgtga tgccagcctc gcaattgagc gacttggcca gaatgcgcga
     6781 gagtgtggtc ttgccgaccc cacgtgtgcc ggtgaacagc caggcgtggt gcaaccggcc
     6841 ggtggacagc gcatgggaca gggcgcgaac tacgtgctcc tggccgataa gggtgtcaaa
     6901 cgagcggggt cgccacttgc gtgccagtac cagataagtc atgcgggagg gttaccctga
     6961 ggctatcgga agacgggaaa caaaaagtgt accggattca ccgacgcttt ggcgggcgat
     7021 tgaaaaa
//
LOCUS       DASTNK010000077        53122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_53711, whole genome shotgun sequence.
ACCESSION   DASTNK010000077 DASTNK010000000
VERSION     DASTNK010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 53122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53122
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53711"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            490..1527
                     /gene="tauA"
                     /locus_tag="VFR20_05840"
     CDS             490..1527
                     /gene="tauA"
                     /locus_tag="VFR20_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_function: GO:0030977 - taurine binding [Evidence IEA];
                     GO_process: GO:0015734 - taurine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0230071.1"
                     /translation="METLFKRARKFVLAGLASGVLALGATTAQAETTVVVGYQQIVGP
                     FVSAIADGSFDRAAQKIGYKIDWRQFSSAGDISTAFASGDVPVGVLGSTGITAAVTRG
                     VDVELFWILDNIGRSEALVARNGSGITKPQDLIGKKVAVPFVSTSHFHLLIALTKLWH
                     INPSKVHILNMQPPAIVAAWQRGDIDAAYVWPPALTEIKKTGKVIIDSAEVGAKSVPT
                     FDGIVANKGWAAKNPKFMAMFTQVLAKSYSDFNAQKSKLTADSPQIKGIVKMIGGKPH
                     DILTALNLLIFPEAKEQASDTWLGGGKDSGAVRAFTASAKFLQSQRQIDKPLNSYTPY
                     VTSAYAAAAAK"
     gene            1629..2456
                     /locus_tag="VFR20_05845"
     CDS             1629..2456
                     /locus_tag="VFR20_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding protein"
                     /protein_id="HEU0230072.1"
                     /translation="MTTSTANLQVSGVSVTYPGLNKTGGSVTALQDVNLEINPGDFVV
                     ALGASGCGKTTLLNLLAGFLSPSRGRLTLGGKPIVGPGSDRGVVFQKHALLPWLNVME
                     NTEFGLKLQGVAKDKRRAIAARNLELVGLQDFHKHMIYQLSGGMQQRVGIARALTCDP
                     AMLLMDEPMAALDALTRETIQELLLDVWQTTHKMFFFITHSVEEALFLGSRLIVMSPR
                     PGRITNTYELDFNKRFLETRDARAIKSSQDFIKMREVVLGIIYGDERAAGEPELQHA"
     gene            2449..3276
                     /locus_tag="VFR20_05850"
     CDS             2449..3276
                     /locus_tag="VFR20_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006395288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0230073.1"
                     /translation="MLSGLFRVKPVAPGKVYGAPGAGHSGFISLMTAVVLIALWFLVT
                     NMGWIKPIFLPSPQAVFHKFIIGVTEGIANSTLWQHTEASLRRVLGGFFLAMITAIPI
                     GILMGVNRVVRGLFDPIIEFYRPLPPLAYLPLVIIWFGIGEFPKTFLIYLAIFAPMAI
                     AARAGVKSVSIEQIHAAYAMGATRQQIIWHVILKAAMPEIFTGMRIGIGVGWTTLVAA
                     EMVASARGLGFMVLNAAQYLASDTVIMGILVIGFFAFIFDLIIRYVEKILIPWKGKV"
     gene            3567..5084
                     /locus_tag="VFR20_05855"
     CDS             3567..5084
                     /locus_tag="VFR20_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU0230074.1"
                     /translation="MQKPAAPSSPILWASLFEQVSDSNISLQGRIRQMIVSAILSGHL
                     PPGSPVPSSRLLSTSLGIARNTVVFAYQQLQSEGYLESRERSGHFVATDVLKGRVGET
                     PRPQTDMPSPLTSRATWHQRLSFQPSAQRNIAKPADWQKLPYPFVYAQFDATQFPTAE
                     WRECCTKALSVLDIRSWAPDLITNDDSALIEEIRTRVLPRRGVWANPEEIVITVGAQH
                     ALYLLADMLVQGHTVVGMEDPGYPDARNTFAHRTPHLLPLPIDEQGLCIGPHLTPCDY
                     LFVTPSHQCPTTVTLPLERREALLAMADEADFIIIEDDYETENRYDNKPTPALKSLDA
                     NGRVIYVGSLSKSFAPGLRIGYIVAPAELVHELRALRRLMLRHPSAYIQRAFALFISL
                     GHHHSLLRRLSLLYEGRAHALVDALDRHLPDFKVRAPSGGSSCWVQAPAGIDTQTLAT
                     RALERGVCIEPGHVFFHNANQHNNNYMRMGFSAIDSQRIEPGIQALSQVYATMRA"
     gene            complement(5096..6526)
                     /locus_tag="VFR20_05860"
     CDS             complement(5096..6526)
                     /locus_tag="VFR20_05860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0230075.1"
                     /translation="MAASPESVRPDVAGAAHSASFNESPYDPLFDPLVSRTGRGQAYA
                     PTYWVGTAGTPPEDDGPVTADMDADVVIIGSGFTGLSTALHLAQEHGIRAIVLEANRA
                     VWGCTSRNGGQGQNASGRLYRSQWIQRWGVDVAKRLDAEIRGGFEYWKSIVATMDCDA
                     QPGGHLYTAHRAKKVPFLENEARVMKEVFGYDTRMLSREELHERYVCDQEAVGALLEP
                     DGVGIHPLKLAFGYIRKARALGVKIHPASPVLGWETVGGVHHLRTPGGVVRARRVAVA
                     TGGYTPQGLHKSLTNKIMPILSNSMVTRPLTSQEREVAGIKTTTFITDTRTLRFYYRL
                     MPDGAMQIGSRAALTGADATNPAHLQLLKDGLARKFPSLKGVPIAYSWWGWVDVSHDM
                     MPRITQPDPDQQIFYAFGYGGNGVSFSTHAGRRLAERVAGKDKKGDHWNLPIYNSPLP
                     GHLFAPFRRLGQAMLYKYYYLRDERF"
     gene            complement(6589..6975)
                     /locus_tag="VFR20_05865"
     CDS             complement(6589..6975)
                     /locus_tag="VFR20_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU0230076.1"
                     /translation="MSDESLTRLLKEFGAAWNRHDIEALMACMHEECVFEAVAGPEVY
                     GARHEGPQAVRKAFESAWLTIPDAQWRNPKVWVKDDQGVMESTFTGTAADGARIEANM
                     VDLIVFRDGKILLKNAFRKNRPPIAA"
     gene            7333..9129
                     /gene="xsc"
                     /locus_tag="VFR20_05870"
     CDS             7333..9129
                     /gene="xsc"
                     /locus_tag="VFR20_05870"
                     /EC_number="2.3.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050487 - sulfoacetaldehyde
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019529 - taurine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
                     /protein_id="HEU0230077.1"
                     /translation="MSTNKTTVPSGPQKMTPSEAFVETMAANGVTEMFGIMGSAFMDA
                     MDIFAPAGIRLIPVVHEQGAAHMADGYARVSGRHGVVIGQNGPGISNCVTAIAAAYWA
                     HSPVVIVTPEAGTMGIGLGGFQEAKQLPMFQEFTKYQGHVTHPARMAEYTGRCFDRAL
                     SEMGPTQLNIPRDYFYGEITAEIPQPQRLERGAGGENSLNAAADLLAQAKFPVIISGG
                     GVVMADGVDQCKALAERLGAPVVNSYLHNDSFPASHPLWCGPLGYQGSKAAMKLIAKA
                     DVVVALGTRLGPFGTLPQHGMDYWPKNAKIIQIDADNKMLGLVKKITVGICGDAKAAA
                     TALTQRLAGRTLACDATKDARAKDIQTEKAAWEKELDDWTHEKDPYSLDMIEEQKKEP
                     GNYLHPRQVLRELEKAMPANAMVSTDIGNINSVANSYLRFERPRSMFAAMSWGNCGYA
                     FPTIIGAKVAAPDRPAISYAGDGAWGMSLMETMTCVRHNIPVTAVVFHNRQWGAEKKN
                     QVDFYNRRFVAGELDNQSFAGIARAMGAEGVTVDRLEDVGPALKKAVDRQMNEGKTTI
                     IEIMCTRELGDPFRRDALSKPVRLLDKYKDYV"
     gene            9234..10253
                     /gene="pta"
                     /locus_tag="VFR20_05875"
     CDS             9234..10253
                     /gene="pta"
                     /locus_tag="VFR20_05875"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU0230078.1"
                     /translation="MDVLDNIISQARLAPRRIVLCEGDDARVLQAGARAHRDRIADIS
                     IVGDPARITARAAEQGVDLSGIDLVEPASSPFFSEAVDTLLELRRARGMTPDTAARAA
                     LDPLAFADLMVRLGHADGSVAGAVHTTADVVRHAIQLIGVKPGNRLVSSFFIMARQEP
                     FHGDTHAMIFSDCGLVIDPDAEQLADIALAAAASARQLLNTEPRVAMLSFSTQGSARH
                     PSVDKVSRAAQLVRERAPDLRLDGEVQLDAAIVPDIARRKIPNSALGGRANVLIFPNL
                     DAANIGYKLTERLGHASAVGPLLQGLNKPANDLSRGCSADDIYNVIAVTAVQAGTSTP
                     TPASK"
     gene            complement(10227..11930)
                     /locus_tag="VFR20_05880"
     CDS             complement(10227..11930)
                     /locus_tag="VFR20_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt chelatase"
                     /protein_id="HEU0230079.1"
                     /translation="MDSARQLGERRRIDDLCAAVLRAATGHAQLHFEAGRLYDDARPV
                     PVRAPHLRVEPRDDGLPARRAVADAVALRLRYSNSALHVDLAPIEPVARLVFEWLEQL
                     RVESLVPDELPGMRGNLERRYREWSAGFFASGSADTSVGVLLFSLSQIVWSRLTSRAL
                     PDEVTDFLEPTRAALAPVLGPSLAGIRRERADQAGFAPHALAIAHEIAQRVQAEIDDE
                     PQAGRSRPGALMLDLEFDQDDTVAFPPVQTATSASYRALGGQYRIFTTRYDQECEASD
                     LVRAGLLREYRDRLDQRIAKQAVNVPRLTRALRAVLALPRRDGWLFGQEEGAVDGRRL
                     AQLVSSPGERRLFRRDRFVPVADAAVALLIDCSGSMRANIESVAVMVDVLMRALEQAG
                     VPTEVLGFTTTAWNGGRPRQEWLSTGRPALPGRLNELRHLVFKSAQTNWRRARRPLAA
                     LLKSDLFREGVDGEAVDWACARLLGQDAGRRVLVVFSDGCPMDSATNQANDDFYLDNH
                     LKAVITRNTRQHGIDIRGVGVGLDLGAYYPRSVAVDLEKGLDNGVFDELVQLLGGRRR
                     R"
     gene            complement(11936..12913)
                     /locus_tag="VFR20_05885"
     CDS             complement(11936..12913)
                     /locus_tag="VFR20_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0230080.1"
                     /translation="MPETSTLSPPVRLVRARELFGFDCRLDIPVFCEPDEHVPDVDPA
                     YRFNEDATLAILAGFAGNRRVMLQGLHGTGKSTHIEQVAARLNWPCVRVNLDGHVSRL
                     DLVGKDAIVVRDGLQVTEFQEGIVPWALQRPVALVFDEYDAGRPDVMFVIQRMLEREG
                     RFTLLDQNRVIHPHPYFRLFATSNTLGLGNLNGLYHGTQLLNHAQLDRWNIVATLNYL
                     SPEEEARIVLARVPELDTDEGHRLVSGMVALAGLTRKGFEVGDLSTLMSPRTVITWAE
                     NSRIFGDIRLAFRLSFLNKCDEAERAIVVEYYQRCFDDNALGSMAPLAE"
     gene            complement(13023..13829)
                     /locus_tag="VFR20_05890"
     CDS             complement(13023..13829)
                     /locus_tag="VFR20_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU0230081.1"
                     /translation="MAIIKQAQNEDPAIDGNTPAMRLFSLLEVIAQKDQFFSLQSLVE
                     ELGLPKPTLHRMLQQLEGAGIIQRDGDGRQYSPGLRLRRMAENLLLNNTVHGARHAVL
                     RQLVEEVGESCNITTCSGDEVLYLDRVETVAPLRFYLRPGSRVPLHCSASGKLFLAQM
                     TQLQRRRLLAQSSLTRYTEKTLVTPEQVEQEIERVRRDGFAKDDEEFLPGLLCVAVMV
                     PVEQGKSNMAVAIQAPIMRMTADKSLSCLPALQRAAQALASIEAANNAEG"
     gene            14012..15088
                     /locus_tag="VFR20_05895"
     CDS             14012..15088
                     /locus_tag="VFR20_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018574136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0230082.1"
                     /translation="MKLGIAGAGLLGRLLALRLSRHGHIVDIFDPAPGPDARGAAGWT
                     AAGMLSPTAELESADMSVFNYGVRSIELWADLLPTLARPVAFSRAGSLLVAHRPDTGA
                     AQRMIRLLDDKAPAGHLPQPISVAELTELEPDVHGPSQAWLLPHEGQLHTVQAMQALQ
                     ASTGDTVTWHWNTTVTRLSARTLHDGRTTREFDHVFDVRGTGARDEGVSETDTAISCR
                     QVRGVRGEIFWVHAPGVRLNRPIRLLHPRHRVYLVPRPGDIVVIGASEIESEDRSPVS
                     LRSTVELLAAAHSVMPALAEARLVHNETNLRPALADNLPRIEHETGLTRINGLFRHGW
                     LIAPAIVEEALTTLNFQHPRTQST"
     gene            15085..15300
                     /locus_tag="VFR20_05900"
     CDS             15085..15300
                     /locus_tag="VFR20_05900"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU0230083.1"
                     /translation="MTTISVTVNGAEHIVAASTTLDDLLRTVFPNAGPNATASAVNGD
                     YVARQARSRYVLNDHDAVTTFEPITGG"
     gene            15303..16103
                     /locus_tag="VFR20_05905"
     CDS             15303..16103
                     /locus_tag="VFR20_05905"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEU0230084.1"
                     /translation="MPFDIAGTSLNSRFFLGTAGYPSPHILRQAIEAAEADVVTVSLK
                     RQLVQGVGKPVQNASGQGFLDIIRQSGAHLLPNTAGCYTADEAITLAQMAREVFDTNW
                     IKLEVIGDSYTLQPDPVELVRAAAALVKLGFEVFPYCTEDLVTCQRLLDAGCRVLMPW
                     GAPIGSGQGLVNPYALRTLRERITDVPLVIDAGIGSPKDAAQAMEMGFDAVLLNSAVS
                     NAHDPALMAEAFKLAIQAGHKARQAGIIAKQDLAVPTTPVTGRPFILA"
     gene            16158..17054
                     /locus_tag="VFR20_05910"
     CDS             16158..17054
                     /locus_tag="VFR20_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEU0230085.1"
                     /translation="MSVLHLVLPDFLLIGLGWALYHRLGFSREFFQNTERLVYFVLFP
                     ALLFHAIARAPLSLGGAATLFEATLALLACGVAMAWLAVPVLRPDPIDHASTAQCAYR
                     FNTYIGLSMAAALGGTAGQATMAVLAGFAVPFANVAAVHALARQRKGGMLGEVARNPF
                     IIATVTGLAFNFLAIPVPAPLYTALGRLGEAAIVMGLLCVGAALSLSGGAQAAKLITW
                     MVSVRLILNPIAAIFIGWIMHLPTLEREILLLFSALPASSSAHVLANRMGGNGRLTAT
                     VMSLGTLISALTIPLWLAIAPH"
     gene            17071..17616
                     /gene="tsaE"
                     /locus_tag="VFR20_05915"
     CDS             17071..17616
                     /gene="tsaE"
                     /locus_tag="VFR20_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU0230086.1"
                     /translation="MRESRNSEPSDLYLQDEEATKAFAMLLAPLLAGRCDGIETGGHI
                     HLHGELGAGKTSFVRALLRALGVTGRIKSPSYAILESYNVFNLYCYHLDFYRFSDPRE
                     WLDAGFREILHKNAVVLIEWPEHAGGLLPPPDLDIHLQYADPGRLASLTAQSDRGRLW
                     LKTLAPRLPDFLPKDSPGANS"
     gene            17547..18902
                     /locus_tag="VFR20_05920"
     CDS             17547..18902
                     /locus_tag="VFR20_05920"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEU0230087.1"
                     /translation="MAENTCPPAAGLPAKGLSRRQLLTVGSTLLILPLIPRAAHAATI
                     LAVRTWPADEYTRVTLELDTELKAEHFVLEHPDRMVVDIEGVSMNHAIKQLVSEVKPN
                     DPYIASIRVAQNRPHVVRLVFDLKQPIAPQIFTLKPVGKYKYRLVLDLYPKVAHDPLM
                     ALTKDSADNDPLAAVLEQLARNSPDQAPIPTVKGQEVPRTARRRNPLPPVAEDRDNPD
                     RPLLIALDPGHGGEDPGATGPQGTHEKNIVLLIGHHLKRLIDAQPNMRAYLTRDKDYF
                     VPLYVRVQKARRVKADLFVSIHADAWIKPSARGSSVFALSRHGATSAAARWMAKREND
                     ADLIGGVDLATHNKQVARVLLDLSTSAQITDSIKVGSRVLDEISKVNRLHSRQVNRAG
                     FAVLKAPDIPSILVETAFISNPQEERLLLNPKHRAKIADAMLAGIKDYFATNPALARR
                     A"
     gene            complement(18997..19635)
                     /locus_tag="VFR20_05925"
     CDS             complement(18997..19635)
                     /locus_tag="VFR20_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEU0230088.1"
                     /translation="MPDILNMILHIDQYMGAWVTQYGLWIYLVLFLIIFCETGLVVVP
                     FLPGDSLLFIAGAFGASELMNPVLLAVLLTAAAVLGNSVNYVIGRLVGPRVFSSNLRF
                     LDRNALIKTHAFYERHGGKTIVLSRFLPLIRTFAPFVAGVAAMNFFRFQMFNISGAVL
                     WVVGLITLGYFFGNVPIIKEHLNTIVLIGVGAAIVPVVLGALWKVCKRASAS"
     gene            19909..21801
                     /gene="mutL"
                     /locus_tag="VFR20_05930"
     CDS             19909..21801
                     /gene="mutL"
                     /locus_tag="VFR20_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU0230089.1"
                     /translation="MSERHHIAALPDTLISQIAAGEVIERPASVLKELLENAVDAGAG
                     NIDVRLDGGGIRRIAVRDDGTGIPREELALALARHATSKIRTLEELESVASMGFRGEA
                     LASIASVARVTLTSRAIGADQAWQISGGDASPSAAAGATGTTVDVRQLFDDVPARRKF
                     LRSEATEYGHCVDALERMALAHPHIGIRVFHNDKPQKQWRAADVTQRMRDILGTDFLG
                     QCLPTEQSHGLITLRGVITRPTYARGRADRQYLYVNGRYVKDRTVSHAVRQAYADVLH
                     GDRQPAYVLFLAVDPGTVDVNVHPAKHEVRFRDSGAVHRFVAQTLGQTLAQAGGPGLP
                     DETAVAQAAGFTPAPASEHVPASQPSPGTHNANSYIPAPRQQAFTLHDAGRDASGWQA
                     AYRPLPVAVGPGAADSVTDGSASESPFDGPAVFQRPTGDSATADDFPLGMALGQLHGI
                     YILAQNRAGLVLVDMHAAHERVVYEQLKHAMDTRGLPRQELLVPVVFNASEKDIGLVE
                     EYQDTLTDLGLTVRPAGPSSLVVRAVPALLASGDIEAMARHVLRDLAAVGSSPRLTEQ
                     RNELLSSMACHGAVRANRRLTLDEMNALLRKMEQTERADQCNHGRPTWVQWPLGDLDK
                     LFMRGQ"
     gene            21798..22730
                     /gene="miaA"
                     /locus_tag="VFR20_05935"
     CDS             21798..22730
                     /gene="miaA"
                     /locus_tag="VFR20_05935"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU0230090.1"
                     /translation="MSIVVPCLTGPTAAGKSASTLALAQRWPIEVINMDSATIYRGMD
                     IGTAKPSTDERARLPQHLLDIRDPAESYSAAAFRQDALALIADIRARGRLPLLCGGTL
                     LYYKTLREGLDDLPQADAALRADIERQAAELGWPALHRQLAILDPATAHRLSPNDSQR
                     IQRALEICRLSGQPMSSLLTRQTRQEEEPYQYVTISLEPSDRSILHARIAQRYLAMMD
                     AGLLAEVAALHARSDLHPGMPSVRCVGYRQLWSYLDGDTTLDDAVQQAIAATRRLAKR
                     QLTWLRSLPDRQVIDCLGAGATSRVVDALSRYWA"
     gene            complement(22739..23788)
                     /gene="purM"
                     /locus_tag="VFR20_05940"
     CDS             complement(22739..23788)
                     /gene="purM"
                     /locus_tag="VFR20_05940"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU0230091.1"
                     /translation="MTDKHSTSFTYRDAGVDIDAGNALVDRIKPLAARTMRAGVMAGI
                     GGFGALFQVPGKYREPVLVSGTDGVGTKLRLAFDWQCHDTVGIDLVAMSVNDILVQGA
                     EPLFFLDYFSCGKLSVDTAASVVGGIAKGCELAGCALIGGETAEMPGMYPDGEYDLAG
                     FAVGAVEKSRIIDGKTIVPGDAVLGLASSGAHSNGYSLLRKILTHANARPGQDLDGRP
                     LRDVVMEPTRIYVKPLLEALSLHSVAIKGMAHITGGGLLENVPRILGDGLAARLHRDA
                     WAMPALFDWLQANGGVADAEMHRVFNCGIGMVVVVAADQADAVVGTLQTQGEQVFRLG
                     EIVEREPGAPQTVVV"
     gene            23869..24573
                     /gene="hda"
                     /locus_tag="VFR20_05945"
     CDS             23869..24573
                     /gene="hda"
                     /locus_tag="VFR20_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEU0230092.1"
                     /translation="MIQQLVLDLLPAPAPTLRNFIAGGNEQVLDALRQCRPGRAMYLW
                     GSPASGRTHLLRALGDPPRGQYFRAPQDASAIRALALDDTAGTSLVAVDDAHLLDEPG
                     QAALFTLYNRWREAASTTQAFALLVSGRQAPLATPLREDLRTRLGWDLVFRLQPLSDE
                     DRARALRTQSTERGLRLSPDVINWMLTHYDRDMSRLGALLDALDHYSVQTHRAITLPL
                     LKDLLASTRERETNPS"
     gene            24570..25271
                     /locus_tag="VFR20_05950"
     CDS             24570..25271
                     /locus_tag="VFR20_05950"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU0230093.1"
                     /translation="MTSPKNLALFDLDHTLLPMDSDYQWADFLARTGRVGDPVLARRR
                     NDELMRLYEAGELTAERAAAFMLGLLTHAPSADLAEWHEHYMVEVVRPNIRPAAVALV
                     RGHLERGDLCAIVTATNEFVTAPIARAFGIPHLIATIPQYRDGRYTGRISGVPSFQAG
                     KVTRVNDWLRGQGLSLSDFETSRFYSDSINDVPLMEAVTDPVATNPSPPLRELAERRG
                     WRVLDLFQEEQATTP"
     gene            25282..26643
                     /gene="pcnB"
                     /locus_tag="VFR20_05955"
     CDS             25282..26643
                     /gene="pcnB"
                     /locus_tag="VFR20_05955"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEU0230094.1"
                     /translation="MLTETIKKFVSRLFSTPTKGPERLARDRHGIDRRNVSRHAIKVC
                     EVLQQHGFAAYIVGGAVRDLIVGLQPKDFDVATNATPEQIRPLFRRARIIGRRFQLVH
                     VVFGQEIIETSTFRAPASDDQETDEHGRILRDNLFGTHEQDAARRDFTINALYYNPMT
                     EEVIDFHHGVADLKNRVVRMIGDPETRYREDPVRMLRAVRFESKLSGTIAPSSREPIK
                     RLAGLIEHVPASRLFDEMLKLLTCGHAMDCLRHLREVGLHNGLLPVIDIIFEQSGGEA
                     FIELALARTDARVRAGKTVSPSFLFASLLWKLVDKRWQARIDQGEPRIQALMDAAESV
                     MEEQTRKLAIQRRYQSDMHEIWFMQPRFERATAKTIWRMLDQPRFRASVDFLQLRAEA
                     REIDSVHAQWWMDLADADDATRADMIETYQRQVRDTPDGARRKRRRTRRTKPQATPDA
                     GEA"
     gene            26640..27194
                     /gene="folK"
                     /locus_tag="VFR20_05960"
     CDS             26640..27194
                     /gene="folK"
                     /locus_tag="VFR20_05960"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU0230095.1"
                     /translation="MKRGSGVSGDSPGQPPHHDLPGTTKIRAYIGLGANLGDAAQTLR
                     HAASQIAESSGITLVALSPVYHTAPIDSSGPDYLNAVAAIDTTLSAMRLLTELQRIEL
                     AHGRTRPYRNAPRTLDLDLLLYGDCAINEPDLVVPHPRMHERAFVLRPLADLAPGLRL
                     RQGSVETLLAGCADQRILRLDEQT"
     gene            complement(27191..28321)
                     /locus_tag="VFR20_05965"
     CDS             complement(27191..28321)
                     /locus_tag="VFR20_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU0230096.1"
                     /translation="MKIRAAVLREMGLPAPYAQSRPVRVSEVELEPPGYGEVLIQIKV
                     AGLCHSDLSVINGDRPRGVPLVLGHESSGEVVEVGAGVNDLRPGDHVVTVFVPSCGCC
                     SPCMAGRPALCEPGARANAQGVLLSGERRLHIGDEYLNHHIGVSCFADYAVVSRRSCV
                     KITAELPHREAALFGCAVLTGAGAVINRAAIKAGQTAAIVGLGGVGLSALLAAVAAGA
                     RRIVAVEINDEKLDLAKTLGATHLINPREITSDTDLLEAAGGPVDFGFDTAGSIPAFE
                     TAYKLTCRGGSTITSGLPHPKLSFSLPLSQMVGEEREIRGSYLGSGVPASDITRYVDL
                     YKAGRLPVDRLLGKAFTLDQINEGFDHLASGSGLRDSIVFGA"
     gene            complement(28597..29817)
                     /locus_tag="VFR20_05970"
     CDS             complement(28597..29817)
                     /locus_tag="VFR20_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0230097.1"
                     /translation="MGKKMTRRWITLALAMMCGGSYAGAAAASPNVILLGSSMQLTGV
                     DANTGHYFRDAYQMAIDRINERGGVKVGGKTYKLALKLYDNQSDVNLSVRQYVQLATK
                     DKVNFLLGPFSSKFTIADSAISEKYRIPMVEDGGASNQIFSRNFKYIFGTLAPATNYF
                     RSTVAMLPHLKPAPKSVALIYADDAFDISVAKGTRALLKAANQNVIMDERYSANTNNF
                     STLLSEIKSKHPDVVLVGGLETEIVNFVRQAKSLNVSPKLYSFTVGVTSEDFRKNLGK
                     DANYAFGMTEWFPNAALKDRWFGDGAQFAKAFEARYHYEPDYHVGSGVAGIETVVNAI
                     EKANSLDPKKVRDAIAASDFDSLYGKVAFNKEGQIALDQTAVQVQDGQIKPIYDGKKF
                     INQAMYPMPAWSAR"
     gene            complement(29892..31070)
                     /locus_tag="VFR20_05975"
     CDS             complement(29892..31070)
                     /locus_tag="VFR20_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEU0230098.1"
                     /translation="MLPLQDITVITLEHAIAAPFCTRQLADLGARVIKIERPGSGDFA
                     RGYDERARGMSSHFVWVNRSKESLALDIKQPEAADILGRLLEKADVLVQNLAPGAAAR
                     LGLSFEALHARFPGLIVCDISGYGDDGPYRDKKAYDLLIQSESGFLSVTGVGDEPAKA
                     GCSIADIAAGMYAYSNILAAIIRRGKTGEGCRIDVSMLESMAEWMSYPLYYSFDGASP
                     PVRSGASHATIYPYGPFTAGDGKTVILGLQNEREWQVFCEQVLHQPELATDPRFSSNS
                     RRSTARGELRALILEAFSSLTIDDVVARLDAAKIANAHMNDMADLWQHPQLRARNRWV
                     DVQSPVGVLPALLPPGVASIEDVRMDAIPALGEHTETILAELGFDGDAIAALRERKAV
                     "
     gene            complement(31085..32245)
                     /locus_tag="VFR20_05980"
     CDS             complement(31085..32245)
                     /locus_tag="VFR20_05980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0230099.1"
                     /translation="MFEAPRQYQDIRDAVRDLCSQFNDEYFRRIDEQRGYPAEFVDAL
                     TKAGWLAALIPQEYGGSGLGLTEASVIMEEINRAGGNSGACHGQMYNMGTVLRHGSEA
                     QKREWLPKIASGDLRLQSMAVTEPTTGTDTTRIKTVAVKKDGRYVVNGQKVWISRVQH
                     SDLMVLLARTTPLEQVKRKSEGMSIFMVDLRKAIGNGLTVRPILNMVNHETNELFFED
                     LEIPEENLIGEEGRGFKYILDGLNAERTLIAAECIGDGYWFVDRARKYVSERIVFGRP
                     IGQNQGVQFPIAKAYVNVEAASLMRYQACALFDSHQDCGAQANMAKMLAADASWEAAN
                     ACLQVHGGFGFACEYDVERKFRETRLYQVAPISTNLILSYVAQHVLGMPRSF"
     gene            complement(32532..33431)
                     /locus_tag="VFR20_05985"
     CDS             complement(32532..33431)
                     /locus_tag="VFR20_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0230100.1"
                     /translation="MAMHFDLTDMQLMVNVAGAMSLTKGAEYTFLSLPAASNRVKNLE
                     TYLGTALLYRTSQGVTLTPSGEAFVRHARIVLRQLEHLRGDIQEYAKGVKGRVRVYAN
                     TTAMNEFIPAALERYLVDHPDVNIELRERLSYQVVKAVAEGVADIGITAQGSGGAGVE
                     FLPYRVDRLVLVTHKDHPLARHAEHSFERTLDYDFIGLFESSAIHSFLLKAAEDSGRI
                     LRMRVEVGNFEASCRLIAAQVGIGVIPESAARRYEKTMPLKIMALTDDWALRKLHICV
                     QEAGKLPAFAQELVDLLVQDAGK"
     gene            33578..34942
                     /locus_tag="VFR20_05990"
     CDS             33578..34942
                     /locus_tag="VFR20_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEU0230101.1"
                     /translation="MQHPSAELSRFAAELRFDDIPEPVLRRAEDLLLDCLASILAGSD
                     SRAVKTLNGYAQAMGPDHGPAENIVGRRGTSPLFAAMVNAAASHMVEQDDVHNGSVFH
                     PAAVVFPPAMATAQTLERSGKDLLVAAVAGYETGIRIGEFLGRSHYTVFHTTGTAGTL
                     ASAMATGRLLGLDPGRMLHALGSAGTQAAGLWEFLRDGADSKQLHTAKAAGDGLMAAW
                     LARDGFTGAAHILEGPQGLAAGMSQDADPARLTDRLGTRWALLETSFKYHASCRHTHP
                     AADALLAIVRDNHLRIDDIASVVAHVHQGAINVLGPVVDPATVHQSKFSMGTVLGLIA
                     KHQRAGLPEFDNALSDPDIIEFRQRVSMRLDHEVDAAYPKRWLGKVTVVTKSGKTLEG
                     RVDTPKGDPDNTLTRPEIEEKAIRLGGYHNAATPAEIQRLITRVWNLADTPRVGTFLG
                     KGEE"
     gene            complement(35398..36222)
                     /locus_tag="VFR20_05995"
     CDS             complement(35398..36222)
                     /locus_tag="VFR20_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEU0230102.1"
                     /translation="MMDTFKMLRTALFVPGTRPERFAKAVASGADAVIIDLEDAVERD
                     AKDIARKNLGQFAADNPHTAFLVRVNDAGAPWFNADLGVCGELPNVVGILLPKAESAE
                     QVGDASAAGKPVFPIVESARGLQALDAIAGAPSVSRLTFGALDLMLDLGITPDTSAAA
                     MLLNQVRYHVLVQSRVHGLNSPIDTIHPDFADTEGLVVAAGQACDMGFGGMLCIHPAQ
                     VEVVHRAFMPSSQELAWARRVVEQADASGSYAFKLDGKMVDLPVIERARRTVARAG"
     gene            complement(36269..37099)
                     /locus_tag="VFR20_06000"
     CDS             complement(36269..37099)
                     /locus_tag="VFR20_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018027268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0230103.1"
                     /translation="MTTSDPASWIGRTESCTDNLDIGHVERVALSLGDAAPAHGEALP
                     LLWHWAFFINAQPYAQLGGDGHPRRGGFLPPADDRNRMWAGGRLKFLRPLLVGVASER
                     MSTIADVTEKEGRTGKLLFVTVKHDYFQDGMLCTAEEQDIVYREPSPPILSGTQPAPE
                     PEWADTIQPTSVMLFRYSAVTFNGHRIHYDEPYVREVEGYPGLVVHGPLIATLMGRAF
                     TRANPGKVVSEFSYRGLRPLIAPRAFQVAGRFESREGAMLWAEQDGTLAHRAEVRFEG
                     "
     gene            complement(37137..38660)
                     /locus_tag="VFR20_06005"
     CDS             complement(37137..38660)
                     /locus_tag="VFR20_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEU0230104.1"
                     /translation="MTTTITESDLIQSVADAVQFISYYHPSDYIQHLARAYEREQNPA
                     ARDAIAQILTNSRMCAEGRRPICQDTGVVNVFLRVGMNVRFDTKQSLQDVCDEGVRRG
                     YTNPDNPLRASILDDPLFTRKNTRDNTPCIVNVELVPGDTVDVQVAAKGGGSENKSKF
                     EMLNPSDSLVDWVLKTVPKMGAGWCPPGMLGIGVGGTAEKAMLMAKQSLMEDIDMYEL
                     LARGPSNKLEELRIALYEKVNALGIGAQGLGGLTTVLDVKIKTFPTHAASKPVAMIPN
                     CAATRHVHFTLDGSGPAVLTPPSLDVWPDVKWAPDYNKSQQVNLDTLTREEVASWKPG
                     QTLLLSGKMLTGRDAAHKRIQEMLDRGEPLPVDFSNRVIYYVGPVDPVRDEVVGPAGP
                     TTSTRMDKFTDMMLERTGLIAMIGKAERGPVAIESIRRHGSAYLMAVGGSAYLVSKAI
                     RHARVVGFEDLGMEAIYEFDVKDMPVTVAVDSSGTSVHETGPREWKERIARINLVPA"
     gene            complement(38763..39530)
                     /locus_tag="VFR20_06010"
     CDS             complement(38763..39530)
                     /locus_tag="VFR20_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEU0230105.1"
                     /translation="MSDLVKVDVVDRILVITLNRPEARNAVNVEVARGLANALDRLDG
                     DDGIALGVLTGAGNSFCSGMDLKAFAQSGTRPYVDGRGFAGICERPPAKPLIAAVEGY
                     ALAGGCEIALACDLIVAAENASFGLPEVKRGLVPGSGGMLRLPRRVPPHIAMEIVLTG
                     AMFPAQRACQYGLVNRLVEPGKALDAALEMARAIAENGPLAVRTAKRILTESPDWRQA
                     DMFDLQRPRVAHIFTSEDAREGATAFAEKRKPIWKGR"
     gene            39737..40648
                     /gene="glyQ"
                     /locus_tag="VFR20_06015"
     CDS             39737..40648
                     /gene="glyQ"
                     /locus_tag="VFR20_06015"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU0230106.1"
                     /translation="MLTFQQIILTLQHYWDKQGCALLQPYDMEVGAGTSHTATFLRAI
                     GPEPWRAAYVQPSRRPKDGRYGDNPNRMQHYYQFQVVLKPAPPEILDLYIGSLMALGI
                     HPDQHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLDCLPTTGEITY
                     GLERLAMYLQNVESVYDLVWTEGRNGHRVLYRDVFHQNEVEQSTYNFELANTTMLFSH
                     FGDYETEAKRLIEAELALPAYEAALKAAHTFNLLDARGAISVTERAAYIGRIRNLSRA
                     VAQGYHDSRERLGFPMLASAPDVEATR"
     gene            40645..42792
                     /gene="glyS"
                     /locus_tag="VFR20_06020"
     CDS             40645..42792
                     /gene="glyS"
                     /locus_tag="VFR20_06020"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU0230107.1"
                     /translation="MSQPGTTHSRPLLVELLTEELPPKALQELGRAFANGIHATLVKQ
                     GLLDADSHIVDFSTPRRLAVRLEQVRAQAPEQAFREKLMPAAVGLTADGQPSPALLKK
                     LAAKGLAHLGPNDLQTESDGKQDYLFAAGTTAGIRLADGLQEALDHAIAHLPIPKFMR
                     YQLADGVTSVRFVRPAHSLLALWGDQIVPVGALGLTASNATQGHRFMSEGSLTITSAG
                     SYEQQLLGEGKVIASFTERRADIDQQLQAQAQRLGASLGDDPEVSRLLDEVTALVEHP
                     TVYVGEFDPEFLGVPPECLILTMRLNQKYFPLFEPDTGKLTHRFLIVSNMRVDDPSNI
                     IEGNQRVVRPRLADAQFFFHTDRKTPLSARVDQLANSVYHNKLGSQLDRVRRIQAIAG
                     HLSQRLGANTGRAERAAWLAKADLNSSMVGEFPELQGIMGAYYAQAEGEPDDVVAAIR
                     GQYRNRIEVPITADSLVPAVLFIAERVETLIGIWGIGLVPTGERDPFGLRRAALGLIS
                     AFEQLRAGGYLNDTATPGLDLPGLLAFATNTFTGHALNPSTPGEVEAFIHERYRNQLA
                     DHDRHVVDAVLALTPPLQQVLDRVRACTDFAQRPEAASLAAANKRITNLLKKASDTQR
                     RFDPTLMREPAEKALAAVIAELEPQARSAFAQGDFVACMATMARARDAVDAFFNDIMV
                     MDEDPAIRANRLALLASLHAVMNLVADISRLTQ"
     gene            42789..43328
                     /gene="gmhB"
                     /locus_tag="VFR20_06025"
     CDS             42789..43328
                     /gene="gmhB"
                     /locus_tag="VFR20_06025"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEU0230108.1"
                     /translation="MKLAILDRDGVINQDSDAFIKSPDEWIPVPGSLEAIARLSRAGW
                     HVVIASNQSGIARGLFTMATLTAIHAKMRRELAKAGGAIDALFVCPHGPDDDCACRKP
                     RPGMFRDIARRYDIALETVPAVGDSLRDLQAASQAGCTPWLVLTGKGQATWNAGDLPA
                     GTQVRNDLAEVVDTWLSGG"
     gene            43334..44062
                     /locus_tag="VFR20_06030"
     CDS             43334..44062
                     /locus_tag="VFR20_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU0230109.1"
                     /translation="MAALRATLYLLFLTITVIPYALACILWSPLPLRWRYRLTAGWPR
                     LAIWGAKTLLGIRWRIKGAENLPDGPAVLLSKHQSAWETLFFPAHMPRQVCFVYKREL
                     HRIPFFGWGLALLRMIPIDRAKGRDAFEQVVRVGRLRLDEGRWPVLFPEGTRVAPGKM
                     GRYKLGGALLAARTGACIIPIAHNAGECWPRNAFIKKPGLITVSIGPAIATDGQDAAT
                     LNRKVQEWIEGEMRELNPERYEPS"
     gene            44049..44927
                     /locus_tag="VFR20_06035"
     CDS             44049..44927
                     /locus_tag="VFR20_06035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEU0230110.1"
                     /translation="MNHPDQLTLPFDLPDTPGAPPALQVPRATDIAPAPLTSCPPLLP
                     QGAKWREVLSGGRPIGFVLQRSKRKTIGLVVGDDGLRITAPKWVTLRQIDDTVLEKSS
                     WILDKLAQHQARQEQLALADTQWRDGGHFPYLGTQIVLVLGSHDGRAAYQGDVMAPQP
                     GDTLRLGLPGDADRNRVRDSAQSWLQQRARAWFGQRLEHFQKVSGRTINRWRLSSAAT
                     RWGSCNSDGNIMLNWRLIHFAPPIIDYVIAHEIAHLREMNHGADFWREVGRILPGFEA
                     ARNTLRQHDPATLPLL"
     gene            44984..45370
                     /gene="gloA"
                     /locus_tag="VFR20_06040"
     CDS             44984..45370
                     /gene="gloA"
                     /locus_tag="VFR20_06040"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEU0230111.1"
                     /translation="MRLLHTMLRVGDLDRSLAFYTNVLGMKVLRRNDYPEGKFTLAFV
                     GYQNESEGAVIELTHNWDTSSYDLGSGYGHIALEVEDAYAACATIKEKGGRVTREAGP
                     MKHGTTVIAFVEDPDGYKIELIQHKR"
     gene            complement(45724..47943)
                     /locus_tag="VFR20_06045"
     CDS             complement(45724..47943)
                     /locus_tag="VFR20_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEU0230112.1"
                     /translation="MNWNGWRRQWLTEPLYRMARSAVPRLSPTEQQAIEAGDVWWEAQ
                     LFSGHPDWQAFGAIKASGLSPEEQVFLDGPVEELCGMLDDWDISWSRYDLPPEAWAFM
                     KRHKFFGMIIPVEYGGLGFSARAHSEIVRRVSLCSVTAGVTVMVPNSLGPGELLMQFG
                     TEAQRGHWLPRLADGREIPCFGLTSPQAGSDAASMTDTGVVCRQMVDGVDTLGIRLNW
                     RKRYITLSPVATVLGLAFKLSDPDHLIGDTSDIGISAALVPTNLPGISIGRRHLPALQ
                     AFQNGPNQGHDVFVPIDALIGGVERAGQGWQMLMAALAAGRGISLPSLSAGACVFAAH
                     TAGCYARVRTQFGIPVGQFEGVQEKLGRLAANAYLVEAARRFTCAGLDMGHKPAVASA
                     IMKLHATERMRQSVNDAMDIHGGKAVIDGPRNYLGGLYRAVPVGITVEGANILTRSLI
                     VFGQGAIRAHPYLMDEVRALADSDQDAGLEAFDHIVWKHLGHSVRNGFRALSSAWFGL
                     PSGTPPAEAGPTAPYYRRLGRYAAAFALVSEATLFSLGGALKRREMLSARLGDALAEL
                     YLLSAVLKRWLDEGRHEPDLPLVRCCVEQGFATIEERLDDVLRNYPSRAMSVALRVVV
                     LPFFRRAKPPSDDVLTSCARILLEPSGTRNRLVGDVWGGHSHAGVEQLEQAFELVTQA
                     QPLLERLRHSGLQDWRIAHEKGAIDDAQAKLLEDAEAAVAAVIEVDDFPAADLPRHR"
     gene            48084..49313
                     /locus_tag="VFR20_06050"
     CDS             48084..49313
                     /locus_tag="VFR20_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HEU0230113.1"
                     /translation="MLNTLWLAFFVIASISGLYHWVVLHDPAVFSRIVLSLFDMADLS
                     VKIMILLFGTLTLWLGFLRIAEAAGLVESLARLLTPLFRRLMPGVPAGHPSIGLITLN
                     FVANALGLDNAATPIGLRAMHSLQSLNPDPKTATNAQILFLVLNSSSLTLLPVTIFMY
                     RAQQGAPDPTLVFLPILLATSASSLVGLLTVAFMQRLKLHDPVVLAWLGGVAIVLGGF
                     MATLATMTSAAISTLSSLMGNLMLFSIIMIFLLVGWRRKVPVYESFVDGAKEGFDIAR
                     NLLPYLVAMLCAIGALRASGALDAALDAIRWCVHSLGLDTRFIPALPTALVKPFSGSA
                     ARAMLIETMKHYGVDSFPALLAATVQGSTETTFYVLAVYFGAVGVQRARHAVGCALAA
                     DIAGVLASIAICYWFFG"
     gene            complement(49397..50713)
                     /locus_tag="VFR20_06055"
     CDS             complement(49397..50713)
                     /locus_tag="VFR20_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0230114.1"
                     /translation="MSDTKVSPRMMRRVVVASVIGNALEWFDFAIFGLFAVVISRLYF
                     PAHSDFASLLLTLATFGVGFAMRPLGGVLLGLYGDRVGRKKALSLTIVLMAVGTGMMG
                     VLPTYQAIGVAAPLLMVLARLIQGFSTGGEFSGATTMLIEFAPAHRRGFYGSFQMCSQ
                     ALAFSCGALSAYLLTAHLTPQALDAWGWRIPFLFGILVGPVGWVIRAHLDESPEFLAY
                     VRNRPQAARESIRASLANLFRDHPREMLATLCICVVGTVSAYVFVFFMPIFAKRQLGM
                     PIADVNLATFVGTAVLLVCCPLAGYLSDRFGRKAVFLPGIIAYGIAGYFLFRHFVQSP
                     SFESLLLAQVMISFFMSFFWGPTPITLTEVFPVGVRSTGASITYNVGVLVFGGLAPLF
                     NTWLVKATGSNMAPIYYVMVSVAIGLVGVLLLPATSSSRAVRPQAA"
     gene            complement(50735..51889)
                     /locus_tag="VFR20_06060"
     CDS             complement(50735..51889)
                     /locus_tag="VFR20_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEU0230115.1"
                     /translation="MLVETEISRLSDEMRGWRRDIHQHPELGFKEHRTADKVAGLLGS
                     WGIEVEAGFGGTTAVVGTLRGKRGPGRCLGLRADMDALPMQEKTDLEYRSVHDNVFHG
                     CGHDGHTAILLGVARHLAAHDDFRGTVHFIFQPAEETLRGGSELIRLGLFDRYPCDEI
                     YALHNNNCIPAGKVGVRCGAILSACDLFRIHIRGVGSHAAMPHKSVDPLLVGSSLVQT
                     LQSVVSRNVDPLDTAVLSICQFHAGSAINVIADTAQLEGTVRTLSRQAQQVVLDRLRE
                     VCDGVAATYGCEVRFEHLLSSPPTINTESETEIVRQAAIRVVGADNVVEAAPLMASED
                     FAFMLEQRPGSYFFLGHNGRTCHHPEFDFDDGSLSTGAEVFMEIVRQRLA"
     gene            52174..52713
                     /locus_tag="VFR20_06065"
     CDS             52174..52713
                     /locus_tag="VFR20_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEU0230116.1"
                     /translation="MGKTNTESGSHDPGFTSYLTFKLDLVKSAMLHRANQVYRRELGL
                     DVRSLRLLRAICDTPGITATRLREITLVEKTLLSKDLTQLVSLKLVRRSIHPDDARHH
                     QLWATAGGKRIRAASDDLGHAMENVMLSMLSSEEHATLNELLGKLSREFLAETGDVDD
                     LIGVLNTHRAKASQRQSKD"
     gene            complement(52760..>53122)
                     /locus_tag="VFR20_06070"
     CDS             complement(52760..>53122)
                     /locus_tag="VFR20_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HEU0230117.1"
                     /translation="QPATSDYGRLSVMLQARYAMEKLFDVAPESFDPPPRVVSAVVRM
                     VPLPASVVAPQHEVAFAAVVARAFSQRRKMLRRALGDWAGLMDWDGLGIPATARAEEL
                     SVAQFARMAERLTEAGAI"
BASE COUNT        10345 a        17909 c        15641 g         9227 t
ORIGIN      
        1 aggcttcgct cggtattgcc tcgcgcacgc atgcgcaggg ggttcaccga cgttaagcgc
       61 attcacaccg gcgctttcga accgggtgcc caatattcag gaggggaaag ttatatccat
      121 ttaactatgg agaccgcgaa ctgctacgta atgccgccaa aaacgaacga attgacggct
      181 atcagccctg attttccgca gactgaccga gccgtgattc taccaccact tcaccaccgc
      241 caagactgac cgcgacagcg ttgcggcaac gatctgaagc ccgacaccgc gctggggttt
      301 tcccgaatcg agttcgcgct ccctcccttt ctccccagtc gctcccaggc tggcaccaac
      361 tggctctaat caatgcaacc atctggacct agtctcactt cacgactatg ggcaagatga
      421 ttgcacgcag cggcacacca agaatcgatg cgcacacaga agcgcacata accaggatgg
      481 agatctgaca tggaaacgtt attcaaacgc gcccgcaagt tcgtcttggc ggggctggcg
      541 agcggggtac tagccctggg cgcaaccacc gcccaagccg aaaccaccgt cgtcgtgggt
      601 tatcagcaaa tcgtggggcc gttcgtcagc gccatcgccg acggcagctt cgaccgcgcc
      661 gcgcaaaaga tcggttacaa gatcgactgg cgacaattca gctcagccgg cgacatatcc
      721 acggcgttcg cctcgggcga cgtgcccgtc ggggtgttgg ggtcgacagg catcactgcg
      781 gctgtcacgc gcggcgtcga tgtggaattg ttctggatcc tcgacaacat cggccgctcc
      841 gaagccctgg tggcgcgcaa cggttccggc atcaccaagc cccaggacct gatcggcaag
      901 aaggtcgcgg tgccgttcgt ctcgacctcg cacttccacc tgctgatcgc gctgaccaag
      961 ctctggcaca tcaacccgtc gaaggttcat atcctgaaca tgcagcctcc ggccattgtg
     1021 gcggcctggc agcggggcga catcgacgcg gcctacgtgt ggccacctgc actgaccgaa
     1081 atcaagaaaa ccggcaaggt catcatcgac tcggccgaag tcggcgccaa gagcgttccc
     1141 acgttcgacg gcatcgtggc caacaagggt tgggccgcca agaacccgaa gttcatggcc
     1201 atgttcaccc aggttctggc caagtcatac agcgacttca acgcgcagaa gtccaaactg
     1261 acggccgact cgccgcaaat caagggtatc gtcaagatga tcggcggcaa gccgcatgac
     1321 atcctcacgg cgctcaacct gctgatcttc cccgaagcca aggagcaggc atccgacacg
     1381 tggctgggcg gtggcaagga cagtggcgcg gtgcgcgcgt tcacggccag cgccaagttc
     1441 ctgcaatccc agcgccagat cgacaagccg ctgaatagct acacgcctta cgtgacaagc
     1501 gcctacgccg cggccgcggc caagtaggtt ccgttggcgc cccgctagac ggcgccttcc
     1561 cggccccacc cgggctccca catgcggggg cccgggtccg gcaaccctcc gaaacaagga
     1621 cccccagaat gactacttcc acggcaaatc tgcaggtcag cggcgtcagc gtgacctacc
     1681 cagggctcaa caaaacggga ggatcagtca ccgcactcca ggacgtgaac ctggaaatca
     1741 atccggggga tttcgtcgtc gcgctcggcg cctcgggttg cggcaagacg acgctgctga
     1801 atctcctggc gggcttcctg tcaccttcac gcgggcggct gaccctcggc ggaaaaccca
     1861 tcgttggccc gggctccgat cgcggcgtcg tgttccagaa acacgccctg ctgccatggc
     1921 tgaacgtcat ggaaaacact gaattcggcc tcaaactgca aggtgtggcc aaggacaagc
     1981 gccgcgccat cgcggcccgc aacctggaac tggtcggctt gcaggacttt cacaaacaca
     2041 tgatctacca gttgtccggg ggcatgcagc aacgcgtcgg catcgcccgc gccctgacgt
     2101 gcgatccggc catgctgctc atggacgaac ccatggcggc gctggacgca ctcacccgcg
     2161 aaaccatcca ggagcttctg ctggatgtct ggcagacgac gcacaagatg ttcttcttca
     2221 tcacccatag cgtagaagaa gcactcttcc tggggtcgcg cctgatcgtc atgtcgccac
     2281 gaccgggccg gatcaccaac acctacgaac tggatttcaa caagcgattc ctggagacac
     2341 gcgatgcacg ggctattaaa tcaagtcaag actttatcaa gatgcgcgaa gttgtcctcg
     2401 gaatcattta cggcgacgag cgggccgccg gcgaaccgga gctccagcat gcttagcggt
     2461 ctgttccggg tcaaacccgt ggcgcccggc aaagtgtacg gcgcccccgg tgccggccac
     2521 agcgggttca tcagcctgat gacggccgtc gtcctgatcg ccctgtggtt cctggtcacc
     2581 aacatggggt ggatcaaacc catcttcctg ccctcgcccc aggcggtctt tcacaagttc
     2641 atcatcggcg tgaccgaagg catcgccaac tcgacgttgt ggcaacacac cgaggccagc
     2701 ttgcggcgcg tcctgggtgg ctttttcctg gcgatgatca cggccatacc gatcggtatc
     2761 ctgatggggg tcaaccgcgt cgtgcgcggc ctgttcgacc ccatcatcga gttctaccgc
     2821 cctctgcctc cgctggccta cctgccgctg gtcatcatct ggttcggcat cggtgagttc
     2881 cccaaaacgt tcctcatcta tctggccata ttcgcaccca tggccatcgc ggcgcgcgcc
     2941 ggagtgaagt ccgtctccat cgaacagatc catgccgcct acgcgatggg agccacccgt
     3001 caacagatca tctggcacgt catcctgaag gcggccatgc ccgaaatctt caccggcatg
     3061 cgtatcggca ttggtgtggg gtggacgacc ctcgtggcgg cggaaatggt ggcatcggcc
     3121 cgaggcctgg gcttcatggt gctcaatgcc gcgcagtatc tggccagcga cacggtcatc
     3181 atgggcatcc tggtcatcgg cttcttcgcc ttcatcttcg acctcatcat acgttacgtc
     3241 gaaaagatcc tcattccctg gaaaggcaag gtctgacacg ctgatcgaca ccagaattcc
     3301 ccggggttcc gcgccagtcc actgctccat ccgacgaagt cggcgcgcct ccggcacttg
     3361 cctcgcccaa gtttcttgcg gcggggcttt tttattccac catgacgcca gcaccgaaat
     3421 tcgcgggagc gcagccccgc atcgcccggc accaacccag aaacattgcc tgtcgcagac
     3481 gtgttggcta tgatcgaccc aaacatcatc gctggccccc cggcgccagc gcgcacagat
     3541 gtcggaacat ccaggcttca acccccatgc agaaacccgc cgcaccgtcc tctcccattc
     3601 tgtgggcttc cctgttcgaa caggtcagcg actcgaacat cagcctgcag gggcggattc
     3661 gccagatgat cgtctcggcg atcctgtcgg gccacctgcc gcccgggtcg ccagtgcctt
     3721 ccagccgcct gctttccacc agcctgggca tcgcgcgcaa caccgtggtg ttcgcctacc
     3781 agcaactgca gtccgaaggc tatctggaat cacgagaacg cagcggccac ttcgtggcca
     3841 ctgatgttct caaggggcgg gtgggcgaaa caccccgccc gcaaaccgac atgccgtccc
     3901 ccctcacatc acgggccact tggcaccagc gcttgagctt ccagccttca gcacagcgca
     3961 acatcgccaa acccgccgac tggcaaaagt tgccctaccc gtttgtctat gcgcagttcg
     4021 acgccaccca attccccacc gctgaatggc gcgaatgctg taccaaggcg ctgtcagtgc
     4081 ttgacatccg aagctgggcg cccgacctga tcaccaacga cgattcggcc ctcatcgaag
     4141 aaatccgcac gcgcgtgcta ccacggcgcg gcgtatgggc caaccctgag gaaatcgtca
     4201 tcacggtggg tgcgcaacat gcgctgtact tgctggccga catgctcgtg cagggccaca
     4261 ccgtcgtggg catggaagac cccggctacc ccgatgcgcg caacacgttc gcccaccgca
     4321 cgccacacct gttgccgctg cccatagacg aacaaggcct gtgcatcggg ccacacctga
     4381 cgccttgcga ctatctgttc gtgacgccca gccaccaatg tcccacgacc gtcacgctgc
     4441 ctctggagcg ccgcgaagcg ctgctggcca tggccgacga ggccgacttc atcatcatcg
     4501 aagacgacta cgaaaccgag aaccgctacg acaacaaacc cacaccggcg ctgaagagcc
     4561 tcgatgccaa tggccgcgtg atctatgtgg gcagcttgtc gaaaagcttc gcgccgggcc
     4621 tgcgcatagg ctatatcgtg gcacccgcgg agctggttca cgaactgcgc gcgttgcgcc
     4681 gcctcatgct acgccacccc tcggcctata tacagcgcgc gttcgcactg ttcatttcgc
     4741 tgggccacca ccactcattg ttgcgccggc tgtcattgct gtatgaagga cgcgcgcatg
     4801 cgctggtgga cgcgctggac cgacatttgc cggatttcaa agtacgcgcc cccagcggcg
     4861 gctcgtcctg ctgggtccag gcgccagccg gcatcgacac gcaaacactg gcgacgcgcg
     4921 cgctggaacg tggagtctgc atcgagcccg gccatgtgtt tttccacaac gccaaccagc
     4981 acaacaataa ctatatgcgc atgggattca gcgccataga cagccagcgc atcgagcccg
     5041 gcattcaagc attgagccag gtttacgcca caatgagggc ttaaaccaaa ccgccctaga
     5101 accgctcgtc ccgcagataa taatatttgt agagcattgc ctgtcccaag cgccggaacg
     5161 gtgcgaacaa atgcccgggc aacggcgaat tgtagatcgg cagattccag tgatcaccct
     5221 tcttgtcctt gcccgcgacc cgctcggcca accgccgacc cgcgtgcgtc gagaacgaca
     5281 ccccgttgcc accatagccg aaggcataga aaatctgttg atcggggtcg ggctgcgtga
     5341 tgcgcggcat catgtcgtgg ctgacatcca cccagcccca ccatgaatag gcgatgggca
     5401 cccccttgag actcgggaac ttccgggcca gaccatcctt gagcaactgc aggtgtgcgg
     5461 gattcgtggc gtcggcgccg gtcagggcgg cacggctgcc aatctgcatc gcgccatccg
     5521 gcatcaaccg ataatagaaa cgcagcgtgc gcgtatccgt gatgaacgtc gtggtcttga
     5581 tgccggccac ctcgcgttcc tggctggtca atggccgggt caccatcgag ttcgacagga
     5641 tgggcatgat cttgttggtc aggctcttgt gcagcccctg tggtgtatag ccgccggtgg
     5701 ccaccgccac gcggcgggca cgcaccacgc cgcctggcgt gcgcagatga tgcacacccc
     5761 ccacggtttc ccagcccagc accgggctgg ccggatggat cttcaccccc agggcgcggg
     5821 ccttgcgtat gtaaccaaac gcgagcttca aaggatggat gccgacaccg tcgggctcca
     5881 gcagcgcacc cacggcctcc tggtcgcaga cataccgctc gtgcaattcc tcgcggctca
     5941 acatgcgggt gtcatagccg aacacttcct tcatgacccg ggcctcattc tcgaggaacg
     6001 gaaccttctt ggcgcgatgc gccgtgtata aatggccgcc gggctgggca tcgcaatcca
     6061 tcgtggcaac gatgctcttc cagtattcga acccgccgcg aatctccgca tcgaggcgct
     6121 tggccacgtc caccccccag cgttgaatcc actgagatcg gtacagccgg cccgacgcat
     6181 tctggccctg tccgccgttg cgactggtgc agccccacac agcccggttg gcttccagca
     6241 cgatcgcgcg gattccgtgc tcctgcgcca agtgcaacgc cgtcgacagc ccggtgaacc
     6301 cggagccgat gatcacgacg tcggcatcca tgtcggcagt gaccggcccg tcatcctcgg
     6361 gcggtgtgcc ggcggtcccc acccaatagg tgggcgcata ggcctggccg cgcccggtgc
     6421 gactgaccag gggatcgaac aggggatcgt aagggctttc attgaacgag gccgagtgcg
     6481 ccgcaccggc gacatcggga cgaacagatt ctggactggc agccatgatg actccgctaa
     6541 tgcattggga accgggcacg acctggcggc gcggcccggg gaaggaattc aggcggcgat
     6601 cggagggcgg ttcttgcgga acgcattctt gagcaggatc ttgccgtcgc ggaacacgat
     6661 caaatccacc atgttggcct cgatgcgcgc gccgtcggcc gccgtgccgg tgaacgtgga
     6721 ttccatgacc ccctggtcat ccttgaccca gactttcggg ttccgccact gcgcgtcggg
     6781 aatcgtgagc catgccgact cgaacgcctt gcgcacggcc tgcggcccct cgtggcgcgc
     6841 gccatagact tccggccccg ccaccgcctc gaacacgcac tcttcatgca tgcaggccat
     6901 gagcgcttcg atatcgtggc gattccaagc ggcgccgaac tccttcagca aacgggtcaa
     6961 cgattcatca gacatcctgt atctccttgt gcgaccacgc ccgaagcgcg tctattgggt
     7021 tttgcatgca gctaagttag gagatcgccc gggggtggtc tagagccaga gtcggcattt
     7081 cgcatacgcc agttaaaccc atgcccatcg ggtgcccctc tggacaccgg catggacact
     7141 gacttttcaa gctctccaag actcaccttg attttataaa acgagataaa aagttccata
     7201 tttaataatt tccattgacc gcaaaaaaaa acaagcatag gatgactaga aattcctgta
     7261 atcggaattt catgttccat cactaaatcg gcgaggaagt ccggccgcca ctgatccatc
     7321 gaggaatccg acatgagcac caacaagacc accgttccct ccgggcccca gaaaatgacc
     7381 ccctccgaag cctttgtcga aacaatggcc gccaacggcg tgaccgaaat gttcggcatc
     7441 atggggtcgg cgttcatgga cgccatggac atcttcgcac ccgctggcat tcgtctcatc
     7501 cccgtggtgc acgaacaagg cgccgcgcac atggccgacg gctacgcgcg tgtgagcggg
     7561 cgccacggcg tcgtcatcgg ccagaacggc ccaggcatca gcaattgcgt cacggccatc
     7621 gcggccgcgt actgggccca ctccccagtc gtgatcgtca cgccggaagc cggcaccatg
     7681 ggcatcgggc tgggtggctt ccaggaagcc aagcaattgc ccatgttcca ggaattcacg
     7741 aaataccagg gccacgtcac acatcccgcc cgcatggcgg aatacaccgg gcgctgcttc
     7801 gaccgtgccc tgtccgaaat gggccccacg caattgaaca tcccccgcga ttacttctac
     7861 ggtgagatca cggctgaaat cccacaaccg cagcgcctcg agcgtggcgc cggcggcgaa
     7921 aacagcctga acgcggcggc tgacctgctc gcccaggcca agttcccggt catcatttcc
     7981 ggtggcggcg tggtcatggc cgatggggtc gaccagtgca aggcgctggc cgaacgcctt
     8041 ggcgcccccg tggtcaacag ctatctgcac aacgactcct tcccggccag ccatccgcta
     8101 tggtgcggtc cgctgggcta ccaggggtcc aaggcggcca tgaagctcat cgccaaggcc
     8161 gacgtcgtcg tggcgctggg aacccgtctg gggcccttcg gcacactgcc tcagcacggc
     8221 atggactact ggcccaagaa cgccaagatc atccagatcg acgccgacaa caagatgctc
     8281 ggcctggtca agaagatcac cgtcggcatc tgcggcgacg ccaaggcggc ggcaaccgct
     8341 ctgacgcagc gcctggccgg ccgcaccctg gcgtgtgacg ccacaaagga cgcccgcgcc
     8401 aaggacatcc agaccgagaa agccgcgtgg gaaaaggagc tcgacgactg gacgcacgaa
     8461 aaagacccgt acagtctgga catgatcgaa gagcagaaaa aagaaccagg caactatctg
     8521 cacccgcgcc aggtcttgcg tgaactggaa aaagccatgc ccgccaatgc catggtgtcg
     8581 acggacatcg gcaacatcaa ctcggtggcc aacagttacc tgcgattcga gcggccgcgc
     8641 agcatgttcg ccgccatgag ctggggcaac tgcggctacg cgttccccac catcatcggc
     8701 gccaaagtgg ccgcgcccga ccgtccggcg atctcgtacg ccggggacgg cgcctggggc
     8761 atgagcctga tggaaaccat gacctgcgtg cgccacaaca ttccggtgac cgcggtggtc
     8821 ttccacaacc gccaatgggg cgcggaaaag aagaatcagg tcgatttcta caaccgccgt
     8881 ttcgtcgcgg gcgaactgga caaccaaagc ttcgccggta tcgcccgggc catgggcgcc
     8941 gaaggcgtga cggtggacag acttgaagac gttggcccgg cgctgaagaa ggcggtcgac
     9001 cggcagatga acgaaggcaa gacgaccatc atcgagatca tgtgcacgcg cgaactgggc
     9061 gacccgttcc ggcgtgacgc attgtccaag ccggtgcgcc tgctcgacaa gtacaaagac
     9121 tacgtctgac gtcacactgc tttacatgtg gccctcgatt catccattga tctcatgtcg
     9181 ctgcgctgat caatggcgag ggcccatttt ttttcaacat ggagccctcg tccatggatg
     9241 tactggataa catcatcagc caggcccgcc tggcgccgcg ccggatcgtc ctctgtgaag
     9301 gcgatgatgc gcgagtcctg caagccggcg cgcgcgcgca tcgcgaccgc atcgccgaca
     9361 tcagtatcgt tggcgacccc gcgcgaatca ccgcgcgggc ggccgaacaa ggcgtggatc
     9421 tgagtggaat cgatctggtt gaacccgcct cgtcgccatt cttttccgag gccgtcgaca
     9481 ccctgctcga attgcgccgc gcacgcggca tgacccctga cacggcagcc agggcggcgc
     9541 tggaccccct ggcgttcgcc gacctcatgg tgcgcctggg ccatgccgat ggctcagtgg
     9601 ccggcgccgt ccacaccacg gcggacgtgg tgcgccacgc cattcaactg atcggcgtca
     9661 aacccggcaa tcgcctggtc tcaagcttct tcatcatggc acgccaggaa ccgtttcacg
     9721 gcgacaccca cgccatgatt ttctccgact gcggattagt catcgacccg gatgcggaac
     9781 aattggccga catcgcgctg gccgccgccg ccagtgcccg ccagttgttg aacaccgagc
     9841 cacgagtcgc gatgctgtca ttctcgacac agggcagcgc acggcatcca tcggtggaca
     9901 aggtatcgcg cgccgcgcaa ctggtccgcg agcgggcccc ggacctgcgt ctggatggcg
     9961 aggtgcaact ggacgccgcc atcgtgcccg acatcgcgcg ccggaagatt ccgaactccg
    10021 ccctgggcgg gcgcgccaat gtgttgatct tccccaacct ggatgcggcg aacatcggct
    10081 acaagctcac cgaacggttg ggtcatgcca gcgccgtggg cccgcttctg caggggctga
    10141 acaaacccgc caacgatctg tcgcgaggct gctccgcgga tgacatctac aacgtcatcg
    10201 cggtcacggc ggtgcaggcc ggcacctcaa cgccgacgcc ggcctccaag taactgaacc
    10261 agctcgtcga atacgccgtt gtccagaccc ttctcaagat ccaccgccac tgagcgcggg
    10321 tagtaggcgc caagatccaa ccccactccc acaccgcgga tgtcgatccc gtgttggcgc
    10381 gtgttgcgcg tgatcaccgc cttcaaatgg ttgtcgaggt agaaatcatc gttggcttga
    10441 ttggtcgcgc tgtccatggg gcagccgtcc gaaaacacca ccagcacccg ccggcccgca
    10501 tcctggccca gcaggcgcgc gcaggcccag tccacggcct cgccgtccac cccttcgcga
    10561 aacaaatcac tcttcagcag cgccgccaat ggacggcggg cgcgacgcca gttcgtctgt
    10621 gccgacttga aaaccaagtg acgcagctcg ttgagccggc ccggcaaagc ggggcgtccc
    10681 gtggacagcc actcttgccg gggccggccg ccattccagg cagtcgtggt gaaaccaaga
    10741 acctcggttg gcacaccggc ctgctcgagc gcccgcatca gcacatcgac catgaccgcc
    10801 actgattcaa tgttggcgcg catcgagcct gagcaatcaa tcagaagcgc gaccgccgca
    10861 tcggcgaccg gaacgaaccg atcgcggcgg aacaggcggc gttcgcccgg cgagctgacc
    10921 aattgcgcca gccggcggcc gtccacagcg ccctcttcct ggccgaacaa ccagccatcg
    10981 cgacgcggca gtgccagaac ggcacgcaac gcgcgggtca gacgcggcac attgacggct
    11041 tgcttcgcga tacgttgatc gaggcggtcg cgatattccc tcagcagacc ggcgcgcacc
    11101 agatccgacg cctcacactc ttgatcgtag cgcgtggtga atatccgata ctggccgccc
    11161 agcgcgcgat acgatgcgct tgtcgccgtc tgtaccggcg ggaacgccac ggtgtcgtcc
    11221 tgatcgaact ccagatcgag catcagggcg cccggccgtg agcggccggc ttgaggctca
    11281 tcgtcgattt cggcctggac ccgttgtgcg atctcgtgcg cgatggcaag cgcgtgcggg
    11341 gcgaagcccg cctggtcggc gcgctcgcgg cgaatgccgg cgagcgaggg tccaagcacc
    11401 ggcgcgagcg cggcgcgggt gggctccagg aaatcggtga cctcatcggg caaggcacgc
    11461 gacgtcaggc ggctccagac aatttgcgag agcgagaaca acaacacccc gacgctggtg
    11521 tcggccgatc ccgacgcgaa gaaccccgcc gaccactcgc gatagcgtcg ttcgagattg
    11581 ccgcgcatgc ccggcaactc atcgggcacc agcgattcga cacgcaattg ctccagccat
    11641 tcgaaaacca gcctggccac cggctcgatc ggtgccagat ccacgtgcaa tgccgaattg
    11701 gaatagcgta gccgcaaggc caccgcgtcg gccaccgcgc gccgggcggg taacccatcg
    11761 tcccggggtt cgacccgaag atgcggtgcg cggaccggca ccggccgcgc gtcgtcgtac
    11821 agccgtcccg cctcgaaatg cagttgcgca tggcccgtgg ccgcgcgcag gaccgcggcg
    11881 cacaggtcgt cgatgcggcg ccgctcgcca agctgtcgcg cgctgtccat ggccgctatt
    11941 ccgcgagagg ggccatcgac cccagtgcat tgtcgtcgaa gcaccgctga tagtactcga
    12001 cgacaatggc acgttcagcc tcatcgcact tgttcaggaa tgacagacgg aatgccagcc
    12061 gaatgtcgcc gaatatccgg ctgttctcgg cccaggtgat cacggtgcgg ggagacatca
    12121 acgtggaaag atcgccgacc tcgaacccct tgcgggtcag cccggccagg gcgaccatgc
    12181 ccgaaaccaa ccggtgcccc tcgtcggtgt ccagctcggg aacccgggcc aggacgatgc
    12241 gggcttcttc ctcgggggag agatagttca aagtggccac gatgttccag cggtcgagct
    12301 gcgcgtggtt gagcaactgg gtaccgtgat acagcccgtt caggttgccc aggcccagcg
    12361 tgttggacgt ggcgaacagc cggaaatacg gatgtggatg gatgacgcga ttctggtcca
    12421 gcagcgtgaa ccgcccctcc cgctccagca tgcgctggat gacgaacatg acgtccgggc
    12481 ggccggcgtc gtattcgtcg aacaccaggg cgacaggccg ctgcaatgcc cagggaacga
    12541 ttccctcctg gaactcggtc acctgcagcc cgtcgcgcac cacgatggcg tctttgccca
    12601 ccagatcgag acggctgacg tgcccatcga gattgacccg cacgcacggc cagttcaggc
    12661 gcgcggccac ctgttcgatg tgggtggatt tgccagtgcc gtgcagaccc tgcagcatca
    12721 cccggcggtt gccggcgaac cccgccagaa tggccagcgt cgcatcctcg ttgaagcgat
    12781 aggccggatc cacgtcgggc acgtgttcgt ccggctcgca gaacaccggg atgtcgagcc
    12841 ggcagtcgaa gccgaacagc tctcgggcgc gcacaaggcg cacgggtggc gacaaagtgg
    12901 aggtttcggg catgatgaat ccacagcaaa aattactgat ccgacagccc gacacgctga
    12961 ttgtgccatc ccgtcaggct tgatggtcaa gctgttgacg ggcggcacac cggcagcgag
    13021 gatcagccct cggcgttgtt ggccgcttcg atgctggcca gcgcctgtgc ggcgcgttgc
    13081 agcgccggca ggcacgaaag agatttatcg gctgtcatgc gcatgatggg cgcctggatg
    13141 gccaccgcca tgttcgactt gccctgttcg accggcacca tgaccgccac acaaagcagc
    13201 cccggcagga actcctcgtc gtccttggcg aagccatcgc gccgcacgcg ttcgatttcc
    13261 tgttcgacct gttcgggggt gaccagggtt ttctcggtgt agcgggtcag ggatgactgc
    13321 gccagcaggc gccgccgctg caattgcgtc atctgcgcca ggaacagttt gccgctagcg
    13381 gaacagtgca agggcacacg cgaaccaggg cgcagataaa accgcaacgg cgccacggtt
    13441 tcgacacgat ccagatacag cacctcgtca ccggagcaag tcgtgatatt gcagctttcg
    13501 ccaacttctt ccaccagttg gcgcaacact gcgtggcgcg cgccatgcac cgtgttgttc
    13561 aacagcaggt tctcggccat gcggcgcagg cgcaggcccg ggctgtactg gcggccatca
    13621 ccgtcgcgct ggatgatgcc ggcgccttcg agctgctgca gcatgcgatg cagcgtgggc
    13681 ttgggcaacc ccagttcctc gaccaggctt tgcagggaaa agaactgatc tttctgagcg
    13741 atgacttcca gcaacgaaaa cagccgcatc gcaggcgtat tgccgtcgat ggcggggtct
    13801 tcgttctgcg cttgctttat aatagccatg gaatatgtct cgttattagg aacatattgt
    13861 accgtttttt tgaaaaaaac gggccgattc ttgccaatct gtccgaattt tacaaaacat
    13921 ccatattccg gaacgtccac ccggccccat cggccggccc ggggccgtca tcaaggcttc
    13981 ggcggtccgg aatcctcgcg ggtctatcgc aatgaaactt ggtatcgctg gagctggcct
    14041 gcttggccgc ctgcttgctc tgcggttgtc ccggcacggc cacatcgtcg acatcttcga
    14101 ccccgcgccg gggccggacg cccggggcgc cgcgggctgg accgccgccg gcatgctcag
    14161 ccccacggcg gaactcgagt cggcggacat gagtgtcttc aactacgggg tgcgctccat
    14221 agagctctgg gccgatttgc ttccgacgct ggcccggccg gtggcgttca gccgcgcggg
    14281 cagcctgctg gtggcgcatc ggccggacac cggcgccgcg caacgcatga tccggttgct
    14341 cgacgacaag gcgccggccg ggcacctgcc ccagccgatc agcgtcgcgg aactgacgga
    14401 gctggaaccc gatgtgcacg gcccctcgca agcctggctg ctgccacacg agggacaact
    14461 gcacaccgtg caggccatgc aagcgctgca agcctccacg ggagacacgg tcacatggca
    14521 ttggaacacc acggtgacac ggctttccgc ccgcacgctg catgacggcc gcacgacccg
    14581 ggagttcgac catgtgttcg atgtgcgcgg caccggcgcg cgcgacgaag gcgtctccga
    14641 gaccgacacc gccatttcct gccgccaggt gcgtggcgtg cgcggtgaga ttttctgggt
    14701 gcacgccccc ggcgtgcggc tgaaccgtcc gatccgcctc ttgcatccgc gccatcgcgt
    14761 ctatctggtt ccgcgacccg gagacatcgt ggtcataggc gccagcgaaa tagaaagcga
    14821 agaccggtct cctgtatcat tgcgatcaac ggtggagttg ctcgccgccg cgcacagcgt
    14881 catgccggca ctggccgaag cgcgcctggt tcataatgaa accaatctgc gtccggcgct
    14941 ggccgacaac ctgccccgca tcgaacacga aaccgggctt acccgcatca atggtctatt
    15001 ccgtcacgga tggctgatcg cgccggcgat cgtcgaagaa gctctcacga ccctgaattt
    15061 ccaacatccg cgcacacaat cgacatgacc acgatttccg tcaccgtgaa tggcgccgag
    15121 cacattgtcg cggcgtcaac gaccctggac gacctgctgc gaacggtgtt tccaaacgcc
    15181 gggccgaacg ccacggccag cgccgtcaat ggcgactatg tcgcgcgcca ggcgcgttcc
    15241 cgctatgtgt tgaacgacca cgacgcggtc acgactttcg aacccatcac cggaggctga
    15301 ccatgccttt cgacatcgca ggcacctcgc tcaacagccg cttcttcctc ggcaccgccg
    15361 gctatccgtc accgcacata ctgcgacagg ccatcgaagc cgccgaggcc gacgtcgtca
    15421 ccgtcagcct gaaacgacaa ctcgtccagg gcgtgggcaa acccgtccag aacgcgtcgg
    15481 gccagggttt cctggacatc atccgccaaa gcggcgccca cctgctgccc aacaccgccg
    15541 gctgctacac ggcggacgaa gccatcaccc tcgcacagat ggcgcgcgaa gtcttcgaca
    15601 ccaactggat caagctggaa gtcatcggcg acagctacac gctgcaaccc gatccggtgg
    15661 aattggtgcg agccgccgcg gcgctggtca agctggggtt cgaagtgttc ccctactgca
    15721 ccgaagacct cgtcacctgc cagcgcctgc tcgatgccgg ctgccgcgtg ctcatgccct
    15781 ggggcgcccc catcggctcc ggccagggcc tggttaatcc ctacgccttg cgcacactgc
    15841 gcgaacgcat caccgacgtg ccgctggtca tcgatgccgg catcggctcc cccaaagacg
    15901 cggcccaggc catggaaatg ggtttcgacg ccgtgctgct gaattccgcc gtctccaacg
    15961 cccacgaccc ggccctcatg gccgaagcct tcaaactcgc catccaggcc ggacacaagg
    16021 cccgccaggc gggcatcatc gcgaaacaag acctggcggt gcccaccacg cccgtcaccg
    16081 gccggccctt catcctggcc tgacgccctg gcccgcccac ccgacaccag acgttttcac
    16141 tctcagaaca ctcacgcatg tccgttctcc atctggttct tcccgacttc ctgctgatcg
    16201 gactcggctg ggcgctgtat caccgcttgg ggttttcgcg cgaattcttc cagaacacgg
    16261 aacgactggt ttatttcgtg ctgtttccgg cgctgctgtt ccacgccatc gcgcgcgcgc
    16321 cgctcagcct gggtggtgcg gccaccctgt tcgaggccac gctggcgctg cttgcatgcg
    16381 gcgtcgccat ggcttggctg gcagtgcccg tgttgcgccc cgaccccatc gatcacgcgt
    16441 caacggcgca atgcgcctac cgcttcaaca cctatatcgg cctgtcgatg gcggccgccc
    16501 tcggtgggac cgccggccag gccaccatgg cggtgctggc ggggttcgcc gtacctttcg
    16561 ccaacgtcgc ggccgtccat gccctggcac ggcaacgcaa gggcggcatg ctgggcgaag
    16621 tcgcgcgcaa cccgttcatc atcgccacgg tcaccggtct ggcattcaac ttcctggcga
    16681 ttccggtgcc ggccccgttg tacacggccc tgggccggct gggcgaggcg gccatcgtga
    16741 tggggctgct gtgcgtcggc gcggcgctgt cgctttccgg cggcgcccag gcggccaaac
    16801 tgatcacctg gatggtgtcg gtgcgcctga tcctgaaccc gatcgcggcg attttcatcg
    16861 gctggatcat gcatctgccc accctggagc gcgaaatact gttgctattc agcgccttgc
    16921 cggcctcgtc gtcggcccat gtcctggcta atcgcatggg gggaaacggg cgcctgacgg
    16981 ccacggtgat gtcgctcggc accttgatct cggccctgac catccctctg tggttggcca
    17041 tcgcccccca ttgacgcgag ccttcccata atgcgagaaa gccggaattc ggaaccatct
    17101 gacctctact tgcaagacga agaggccacg aaagcgttcg ccatgttact ggcgccattg
    17161 cttgccggtc gctgcgacgg catcgagacg ggtggccaca tccatctgca tggcgagctt
    17221 ggcgccggca aaaccagttt cgtccgagcc cttttgcggg cccttggcgt caccggaaga
    17281 ataaaaagtc ctagctacgc catccttgaa agttataatg tttttaactt atactgctat
    17341 caccttgatt tctataggtt tagcgatcca cgcgaatggc tggatgctgg gttcagagaa
    17401 atcctgcaca aaaacgcagt ggttttgatc gaatggcccg agcatgcggg gggtttgctt
    17461 cccccgcccg atctcgacat ccatcttcag tatgccgacc ccggacgact ggccagcctg
    17521 accgcacaaa gcgaccgagg acgactatgg ctgaaaacac ttgccccccg gctgccggac
    17581 ttcctgccaa aggactctcc cggcgccaac tcctgacggt cggctccacg ctgctgattc
    17641 tcccactgat tccgcgcgca gcgcacgccg ccaccatcct ggccgtccgc acttggccgg
    17701 ccgacgaata cacccgggtc accctggaac tcgacaccga gctcaaggcc gagcatttcg
    17761 tgctcgagca ccccgatcgc atggtggtcg acatcgaagg ggtgtccatg aaccatgcca
    17821 tcaagcaact ggtgtcggag gtcaaaccga acgaccccta catcgcctcg atacgggtgg
    17881 cgcagaaccg gccccatgtg gtgcgcctgg tattcgacct gaaacagccc atcgcaccgc
    17941 agatcttcac gctgaagccg gtgggcaagt acaagtatcg actggtgctg gatctttacc
    18001 ccaaggtcgc tcatgatcca ctgatggccc tcaccaagga cagcgccgac aacgatccgc
    18061 tggccgccgt cctggagcaa ctggcccgga attcaccgga ccaggccccc atccccactg
    18121 tgaaggggca agaagtccca cgcaccgcgc ggcgccgcaa cccgctgcca cccgtcgcgg
    18181 aggaccgcga caaccccgac cggccactgt tgatcgcgct cgatccgggc cacggtggcg
    18241 aagaccccgg cgccaccggt ccacagggca cccacgagaa gaacatcgtt cttctcatcg
    18301 gccaccatct gaagaggctc atcgacgcgc aacccaacat gcgcgcctat ctcacgcgcg
    18361 acaaggatta cttcgtgccc ctgtacgtgc gtgtacagaa ggcgcggcgc gtcaaggccg
    18421 acctgttcgt gtctatccac gccgacgcct ggatcaagcc cagcgcccgg gggtcttccg
    18481 tattcgcgct atcgcgtcac ggcgccacca gcgccgccgc gcgctggatg gccaagcgcg
    18541 agaacgacgc cgacctgatc ggcggcgtgg atctagcgac tcacaacaag caggtggccc
    18601 gggtgctgtt ggatctctcg acgtctgcac agatcacgga ttcgatcaag gtgggaagcc
    18661 gggtgctcga cgagatctcc aaggtcaatc gcttgcacag ccgacaggtg aaccgcgcgg
    18721 gattcgcggt gttgaaggcg ccagacatcc catccatcct ggtcgagacg gccttcatca
    18781 gcaacccgca ggaagagcgc ctgttgctca acccgaaaca ccgggccaag atcgccgacg
    18841 cgatgctcgc cggcatcaag gattatttcg cgaccaaccc ggccctcgcc cggcgcgcgt
    18901 gacattgacc tgagcgacac acagtgcgcc gcgacggcat ggaacccgtg tgtgaggcga
    18961 cccggccagg cccgggtcgc gcaacgcgcc agcggatcag gacgccgacg cgcgcttaca
    19021 gaccttccac aaggcgccca ggaccaccgg cacgatcgcc gccccaaccc cgatgagcac
    19081 gatcgtgttc aggtgctctt tgataatggg tacgttgccg aagaagtagc ccagggtgat
    19141 caacccgacc acccacagca ccgccccgct gatgttgaac atctggaagc ggaagaaatt
    19201 catggccgcc acgccggcca cgaaaggcgc gaatgtgcgg atcaagggaa gaaaacgcga
    19261 caacacaatg gtctttcccc cgtgcctttc atagaacgca tgggttttga tcaaggcgtt
    19321 gcgatccagg aaccgcagat tgctggagaa cacgcggggt ccgacaagcc gcccaataac
    19381 gtaattcacg gaattgccta gaaccgcggc ggcggtcagc aagaccgcca gcaatacggg
    19441 attcatcagt tccgacgctc caaaggcgcc ggcgatgaac agcagcgaat cgcccggcag
    19501 gaacggcacc acgaccaagc cggtttcgca gaagatgatc aggaacagca cgaggtagat
    19561 ccagagcccg tactgtgtca cccacgcgcc catgtactgg tcaatgtgga ggatcatgtt
    19621 caggatatcg ggcatatggg tacactaccg gaagacggtg gccgggcctg ggcgcaggcc
    19681 ggccggaagc tgcaatatag cccaaacatc cgcgccggcg aaatcctcgg ccgccacaga
    19741 tgtaacgggg cgcaagacta taccgggcaa gccgtccgct cgatgcgcct ctataaatgg
    19801 acggatgggg tcctgcctcg ggcgcactgc ggcgttgcct taaaatcgtc atggcgtcgt
    19861 cccgccgatg tgcccttttc catccgcttc ccgccaacac aagacctcat gtccgaacgc
    19921 caccacatcg ccgcactgcc ggatacgctg atcagtcaga tcgccgccgg cgaggtcatc
    19981 gaacgaccgg cgtcggtgtt gaaagaactg ctcgagaacg ccgtcgatgc cggggccggc
    20041 aacatcgacg tgcgcctcga cggcggcggc atacgccgca tcgccgtgcg cgacgacgga
    20101 accggcatcc cccgggaaga gctcgcactg gcgctggccc gccacgccac cagcaagatc
    20161 cgcacccttg aagagctcga atcggtggcg tccatgggct ttcgagggga ggcactggcc
    20221 tcgatcgcat cggtggcgcg ggtcacgctc acctcgcgcg caatcggtgc ggaccaagcc
    20281 tggcagatca gcggcggcga cgcatccccc agcgcggccg ccggtgccac cggcaccacc
    20341 gtggatgtcc gccaactgtt cgacgacgtc ccggcccggc gcaagtttct gcgatcggag
    20401 gccacggaat acggccattg cgtggacgcg ctcgagcgca tggcgctggc gcacccgcac
    20461 atcggcatcc gggtgttcca taacgacaag ccgcagaagc aatggcgcgc cgcggacgtc
    20521 acgcagcgca tgcgcgacat cctcggcacc gacttcctgg ggcaatgcct gcccacggaa
    20581 caatcccatg gcctgatcac cctgcgcggt gtcatcacgc ggcccacgta tgcccgaggc
    20641 cgggccgaca ggcagtacct ctacgtcaac ggacgctacg tcaaggatcg cacggtatcg
    20701 cacgccgtgc gacaggccta tgccgatgtc ctgcacggcg atcgccagcc ggcctatgtg
    20761 ttgttcctgg ccgtggatcc gggtaccgtc gacgtcaacg tgcaccccgc caagcatgaa
    20821 gtccggttcc gcgacagcgg cgccgtgcat cgattcgtgg cccagaccct gggccagaca
    20881 cttgcgcaag ccggcggccc cggcttgccc gatgagaccg cggtcgcaca ggcagccggt
    20941 ttcaccccgg cccccgcctc ggaacacgtc cctgccagcc aaccctcccc gggcacgcac
    21001 aacgccaata gctacattcc cgcaccgcgt caacaagcct tcacgctaca cgatgccggc
    21061 cgtgacgcga gtggctggca ggcagcctat cgcccactgc cggtcgcggt gggccctggg
    21121 gcggcggatt cggtcacaga tggttccgcg tcggagagcc ccttcgacgg gccagccgtt
    21181 ttccaacgcc caacaggcga cagcgccacc gcggatgatt tccctttggg catggcgctg
    21241 gggcaactgc acggcatcta catcctggcg cagaatcgcg cgggcctcgt gctggtcgac
    21301 atgcacgccg cccacgaacg ggtggtctac gaacaactga aacacgccat ggacacccgc
    21361 ggcctgccgc gccaggaatt gcttgtcccc gtggtgttca acgcgtcgga aaaagacatc
    21421 ggcctggttg aggaatacca ggacaccctg accgacctgg gtctgacggt ccgccccgcc
    21481 ggcccgtctt cgctcgtggt gcgcgcggtg cccgcgctgc tggccagtgg cgatatcgaa
    21541 gccatggcgc gccacgttct gcgcgacctg gccgccgtgg gcagttcgcc gcgcttgaca
    21601 gaacagcgca acgagttgct ctccagcatg gcttgccatg gcgcggtgcg cgccaatcgc
    21661 cgcctgaccc tggacgaaat gaacgccctg ctgcgcaaga tggagcagac cgaacgggcc
    21721 gaccaatgca accacggacg ccccacctgg gtgcaatggc cgctgggcga cctcgacaag
    21781 ctgttcatgc gcggccaatg agcatcgtcg tcccctgcct gaccggcccc accgccgccg
    21841 gcaagagcgc gagcacgctt gcattggcgc aacgctggcc gatcgaggtc atcaacatgg
    21901 actcggccac gatctatcgt ggcatggaca tcggcacggc gaaaccctcg acggacgagc
    21961 gcgcgcgcct gccccagcat ctgctcgaca tccgcgaccc ggccgagagc tattccgcag
    22021 cggccttccg gcaagacgcc ctggcgctca tcgccgatat tcgcgcgcgc ggccgcctgc
    22081 ccttgctgtg cggcggcacg ctgctgtact acaagacctt gcgtgagggc ctggacgatc
    22141 tgccgcaggc cgatgcggca ctgcgcgccg acatcgagcg gcaagcagcg gaactgggtt
    22201 ggcccgccct gcaccgccag cttgccattc tggatccggc caccgcgcac cgcctgtcgc
    22261 ccaacgacag ccaacgcatc caacgagccc tggaaatctg ccgcctgagc ggccagccca
    22321 tgtccagcct gctgacccgc cagacacgtc aagaggaaga accgtaccaa tacgtcacga
    22381 tcagtctgga gccttccgat cggtcgatac tgcatgcgcg catcgcgcag cgctatctgg
    22441 ccatgatgga tgccgggctg ctggctgaag tcgccgccct gcacgcacgc tccgacttgc
    22501 atcccggaat gccatcggtg cgctgcgtgg ggtatcgcca gctatggagt tatctggatg
    22561 gcgacacgac gctggacgat gccgtccagc aagccatcgc cgccacccgg cgcctggcca
    22621 aacgccaact gacctggctg cgttcgctgc cagatcgcca ggtgatcgat tgccttggag
    22681 ccggcgcgac cagccgcgtc gtcgatgccc tgtcacggta ctgggcttga gcggagaatc
    22741 agaccaccac ggtctgtggc gcgccgggtt cacgctcgac gatttcaccc aaccggaaca
    22801 cctgctcacc ctgggtctgg agcgtgccca ccacggcatc ggcttgatcg gccgcgacga
    22861 cgacgaccat gcctatgccg caattgaaca cgcgatgcat ctcggcgtcg gccacaccgc
    22921 cgttggcctg cagccagtcg aacagcgccg gcatggccca ggcgtcacgg tgcagccgcg
    22981 ccgcaaggcc gtccccgaga atacggggca cgttctcgag caagccaccg ccggtgatgt
    23041 gcgccatgcc tttgatggcg acactgtgca gggacaaggc ctcgagcaag ggtttgacat
    23101 agatgcgcgt cggctccatg accacatccc gcaaggggcg tccgtccagg tcctggccag
    23161 gccgcgcgtt ggcgtgcgtc aggatcttgc gcaacagcga atacccattg gaatgcgcac
    23221 ccgacgaagc caaccccagg accgcgtcgc ccggcacgat ggtcttgcca tcgatgatgc
    23281 gcgatttttc gaccgccccc accgcgaaac ccgccagatc gtactcccca tcgggataca
    23341 tgccaggcat ctcggcggtt tcccctccga tgagcgcgca gccggccaat tcgcagcctt
    23401 tggcaatgcc gcccaccacg ctggccgcgg tatccaccga cagtttgccg caggaaaaat
    23461 aatccaggaa aaacaggggt tccgccccct gaaccaggat gtcgttgaca ctcatggcca
    23521 ccagatcgat ccccacggtg tcgtggcact gccagtcgaa ggccaggcgc agcttggtcc
    23581 ccaccccgtc ggtccccgac accaacaccg gctcccggta cttgcccggc acctggaaca
    23641 gggcgccgaa cccaccgatc ccggccatga cgccagcgcg catcgtgcgg gccgccagcg
    23701 gcttgatacg gtcgaccaat gcgttaccgg cgtcgatatc gacgccggca tcgcgatagg
    23761 tgaatgaggt ggagtgcttg tcggtcatga gaaatatcgc cagatgtgca agaattgcgt
    23821 gttggttaag ccgtcacggc gcattttacg tccttttgcc gcaccttaat gattcagcaa
    23881 ctcgttctcg acctgcttcc cgccccggcc cccacgctgc ggaatttcat cgccggcggc
    23941 aatgagcagg tgctggacgc gctgcgccag tgccgccccg gccgcgccat gtatctgtgg
    24001 ggttcgccgg cttcgggccg cacccacttg ctgcgcgccc tgggtgaccc cccgagggga
    24061 cagtacttcc gcgctccgca agacgcgtcg gccatccggg ccctggcgct ggacgatacg
    24121 gccggcacga gcctcgtggc cgttgacgat gcccatttac tggacgaacc cggccaggcg
    24181 gccttgttca ctctctacaa ccgctggcgg gaagcggcct ccaccaccca ggcattcgcg
    24241 ctgctggtgt cggggcgcca ggcaccgctc gccaccccgc tgcgggaaga cctgcgcacc
    24301 cgtctgggct gggacctggt gttccgcctg cagccgctgt cggacgaaga ccgcgcccgc
    24361 gccctgcgca cgcagtcgac ggagcgcggc ctgagacttt cccctgatgt catcaactgg
    24421 atgctgaccc actacgaccg cgacatgagc cggctgggcg ccctgctcga cgcgctcgat
    24481 cattactccg tgcagacgca ccgggccatc accctgcctc tgttgaaaga cctgctcgcc
    24541 tcgacccgtg agcgcgaaac gaacccatca tgacatcccc gaaaaatctg gccctgttcg
    24601 acctcgacca caccttgctg cccatggaca gcgattatca atgggcggac ttcctcgccc
    24661 gcacgggcag ggtgggcgac ccggtcttgg cccggcgccg caacgacgag ctcatgcggc
    24721 tctatgaggc cggcgaactc accgccgaac gcgccgcggc cttcatgctg ggcctgctga
    24781 cccacgcccc cagcgccgac ctggcggaat ggcacgaaca ctacatggtg gaggtcgtgc
    24841 gccccaacat ccgccccgcc gccgtggcgc tcgtgcgcgg ccacctcgag cgcggcgatc
    24901 tatgcgccat cgtcaccgcc accaacgaat tcgtcaccgc ccccatcgcc cgggcgttcg
    24961 gcattcccca tctgatcgcc accatccccc agtaccgtga tggacgctac accgggcgga
    25021 tcagtggcgt cccgagtttt caggcgggca aagtcacccg ggtgaatgat tggctgcgcg
    25081 ggcaaggcct gtcgttgagc gacttcgaga cgtcccgttt ctacagcgac tcgatcaatg
    25141 atgtgccgct catggaagcc gtgaccgatc ctgtcgcgac caaccccagc ccccctctga
    25201 gagagctcgc cgagcgccgt ggatggcggg ttctggatct ttttcaggaa gagcaggcca
    25261 ccacacctta aaataccgcc catgcttact gaaaccatca agaaattcgt cagccgcctg
    25321 ttctcgaccc cgaccaaagg gcccgagcgc ctggcccgcg accgccacgg catagaccgg
    25381 cgcaatgtct cgcggcacgc catcaaggtc tgtgaagtgt tgcagcaaca tgggttcgcc
    25441 gcctacatcg tcggcggcgc ggtgcgcgac ctcatcgttg gactgcagcc caaggatttc
    25501 gacgtcgcca ccaacgccac accggaacag attcggccgc tgttccgccg cgcgcgcatc
    25561 atcggtcggc gcttccagct cgtgcacgtg gtgttcggcc aggaaatcat cgaaacctcg
    25621 acctttcgcg cccccgcctc ggacgaccag gaaaccgatg agcacggccg cattctgcgc
    25681 gacaacctgt tcggcacgca cgaacaagac gccgcgcggc gcgacttcac catcaacgcg
    25741 ctgtactaca accccatgac cgaggaagtc atagacttcc atcacggcgt ggccgacctg
    25801 aagaatcgcg tcgtgcgcat gatcggcgat ccggaaacac ggtaccggga agaccccgtg
    25861 cgcatgctgc gtgccgtgcg tttcgaatcc aaactcagcg gcaccatcgc cccgtcttcg
    25921 cgcgaaccca tcaaacgcct ggcgggcctc atcgaacatg ttccggcctc ccgtctgttc
    25981 gacgaaatgc tcaagcttct gacctgtggt cacgccatgg actgcctgcg gcacttgcgc
    26041 gaagtcggcc tgcacaatgg cctgctgcct gtcatcgaca tcatcttcga acaatccggt
    26101 ggcgaggcat tcatcgaact cgccctcgcc cgcacagacg cccgcgtgcg cgcgggcaaa
    26161 acggtgagcc ccagcttcct gttcgcatcc ctgctgtgga agctggtcga caagcgctgg
    26221 caggcgcgca tcgaccaggg agaaccccgc atccaggcgc tcatggacgc cgccgagtcg
    26281 gtcatggaag aacagacacg caagctggcc atccagcgac gctaccagtc cgacatgcac
    26341 gaaatatggt tcatgcaacc gcgcttcgag cgcgccaccg ccaagaccat ctggcgcatg
    26401 ctggaccaac cgcgctttcg cgcatcggtc gactttctgc aactgcgcgc cgaagcgcgt
    26461 gaaatcgaca gcgtgcatgc ccaatggtgg atggatctcg ccgacgccga cgacgccacc
    26521 cgcgccgaca tgatcgaaac ctaccagcgc caggtccgcg acacgcccga tggcgcgcgc
    26581 cgcaagcgcc ggcgcacgcg gcgaaccaag cctcaggcca caccggacgc tggcgaggca
    26641 tgaagcgggg ctcaggtgtg tccggcgact cgccgggcca gcctccacac cacgacttgc
    26701 cgggtactac caagatacgt gcctacattg ggctcggcgc caatcttggc gacgcggcgc
    26761 agaccctgcg acatgccgcc agccaaatcg ccgaatcctc cggcataacc ctggtggcgc
    26821 tgtccccggt gtatcacaca gcgccgatcg attcgagcgg gccggactac ctcaacgcgg
    26881 tggcggcgat cgacaccacg ctttccgcca tgaggcttct gaccgagctg cagcgcatag
    26941 agctggccca cggccgcacg cgcccgtatc gcaacgcccc gcgcaccctc gacctcgatc
    27001 ttctgttgta cggcgactgc gccatcaacg agccggacct cgtcgtaccc catcccagaa
    27061 tgcacgaacg ggccttcgtg ttgcgcccgc tcgccgacct cgccccgggc ctgcgtctgc
    27121 ggcaggggtc ggtggaaaca ttgttggcag gatgcgccga tcagcggatt ctgcggctcg
    27181 atgagcagac ttaagctccg aacacaatcg aatcgcgcaa gcccgaaccc gaagccagat
    27241 gatcgaaacc ctcgttgatc tgatccagcg tgaacgcctt gccaagcagc ctgtccaccg
    27301 gtagccggcc cgctttgtac agatcgacat atcgtgtgat gtctgacgcc ggcacgcccg
    27361 accccagata gctcccgcga atctcacgtt cttcgcccac catctgcgac agcggcaacg
    27421 aaaaactgag cttggggtgc ggcagccccg aggtgatggt cgaaccgcca cggcatgtaa
    27481 gcttgtacgc ggtctcaaac gctggaatcg acccggccgt atcaaacccg aaatcaactg
    27541 gcccgcccgc cgcctcgagc aaatctgtat cagaagtgat ttccctggga ttgatcagat
    27601 gcgtggcacc caaagtcttg gccagatcga gcttctcatc attgatctcg acggcgacta
    27661 tgcgccgggc ccccgcagcc accgccgcca gcagcgcgct caggccgaca ccacccagcc
    27721 cgacaatcgc ggccgtctgg ccagccttga tagccgcccg gttgatcacc gcgccggcgc
    27781 ctgtgagcac agcacagcca aacaaggccg cctcgcgatg cggcaactca gcggtgatct
    27841 tcacgcaaga acgtcgcgac accacggcat agtccgcgaa gcacgacacg ccgatatgat
    27901 gattgagata ctcgtcaccg atatgcagac gacgctcgcc gctcagcagc accccttgcg
    27961 cgtttgcccg cgcaccgggt tcgcacaatg ccggccgccc cgccatgcaa ggcgaacaac
    28021 aaccacagct cggcacaaac actgtgacca cgtggtctcc cggccggaga tcattcacgc
    28081 cggcaccaac ctccacgacc tccccggacg attcatggcc aagcaccagg ggaacaccgc
    28141 gcgggcggtc accgttgatg acggacaagt ctgaatggca caaccccgcc accttgatct
    28201 ggatcagaac ctctccatac ccgggcggct ccagttcgac ctcgctcacc cgtactggcc
    28261 ggctttgcgc ataaggcgcg ggcagaccca tttcacgcaa cactgctgcg cgtatcttca
    28321 tgcagtatct cctcttattg tgggtggacg aaacgacgga tgacccgctt ggggcatccc
    28381 acacaaagga catcaccaag gattctaccc ctcgaccgct cccgtgtgac cgcgcctgag
    28441 cggttcgccc gcactcatgc cggagagggc ttcaagcatc aaaaccactc catggcggga
    28501 aacaaaacgg gcaccgcctc cccatagatc ctttgtcggg agggcggtgc cctagggcac
    28561 agttcggatc gcggctaggc gaaaagtggt gaccgcctat cgcgccgacc aggccggcat
    28621 cggatacatc gcttgattga tgaatttctt gccgtcgtag atgggcttga tctggccgtc
    28681 ctgcacctga accgcggttt gatccagggc tatctgcccc tccttgttga acgcgacctt
    28741 gccatacaag ctgtcgaaat cactggcggc aatggcatcg cgcactttct tcggatccag
    28801 ggaattggcc ttttcaatgg cgttgaccac ggtttctatg cctgccacgc cggatccgac
    28861 gtggtagtcg ggctcgtagt gatagcgggc ttcgaatgcc ttggcgaact gggcgccatc
    28921 gccgaaccaa cggtccttga gcgccgcatt ggggaaccat tccgtcatgc cgaacgcata
    28981 gttcgcatcc ttgcccaggt tcttgcggaa gtcctcgctg gtgacgccca cagtgaatga
    29041 gtacaacttg ggcgagacgt tcagactctt ggcctggcgc acgaaattca cgatctccgt
    29101 ctcgagccca cccaccagca cgacatccgg gtgcttcgac ttgatctcgg aaagcaaggt
    29161 gctgaagttg ttggtgttgg cgctgtagcg ctcgtccatg atgacgttct ggttcgccgc
    29221 cttgagcagc gcccgcgtgc ccttggccac ggatatgtcg aacgcgtcgt ccgcgtagat
    29281 cagggccacg gatttgggcg cgggcttcaa atgcggcaac atggccaccg tgctgcggaa
    29341 ataattggtc gccggcgcca gcgtgccgaa aatatatttg aaattacgcg agaagatctg
    29401 attggaagcg ccgccatcct ccaccatggg aatccgatat ttctcggaga tcgccgagtc
    29461 ggcaatggtg aatttgctgg aaaacggccc gagcaggaaa ttgaccttgt ccttggtcgc
    29521 cagttgcacg tactgtcgta cgctcagatt cacatcggac tggttatcgt aaagcttcag
    29581 cgccagtttg tatgtctttc cgccaacctt gacgccaccc cgctcgttga tcctgtcgat
    29641 tgccatctgg tatgcatcgc ggaaatagtg gcccgtgttg gcatccacac ccgtcaattg
    29701 catcgatgac ccaagcagga tcacgttggg cgaggccgcc gccgcacccg cataggaacc
    29761 gccacacatc atcgcaagcg ccagggtaat ccaacgccgc gtcatcttct ttcccatcaa
    29821 aaaacctcct tggttgacat cccccgatac cgctcttttt atatggccgg cgatcgcgcc
    29881 ggcctgttat ctcaaaccgc cttccgttca cgcaacgcgg caatggcatc gccatcgaaa
    29941 cccagctcgg cgagaatggt ctctgtatgc tcacccaggg ccggaatggc gtccatgcgc
    30001 acgtcttcga tactggcaac gcccggtgga agcagcgcag gcaacacacc aaccgggctc
    30061 tgcacgtcga cccagcggtt tcgcgcgcgc aattgcgggt gttgccaaag gtcggccatg
    30121 tcgttcatat gcgcattggc gatcttcgcc gcgtccaggc gcgcgacgac gtcgtcgatt
    30181 gtcagggagg aaaacgcctc gagaatgagg gcgcgcagtt ctccccgcgc ggtcgatcga
    30241 cgcgagtttg acgaaaaacg cggatcagtc gccagttccg gctgatgcag cacctgctcg
    30301 cagaaaacct gccattcccg ttcgttctgc aggcccagta tcactgtctt gccatcgccc
    30361 gcggtgaatg gcccataagg atagatggtg gcgtgcgatg cgccgctgcg cacgggtggc
    30421 gaggccccat cgaagctgta gtacagggga taagacatcc attcggccat gctctcgagc
    30481 atggagacgt cgatgcggca cccctccccc gttttgcccc gccggatgat ggcggccagg
    30541 atgttggaat aggcgtacat gccggctgcg atatcggcaa tggagcagcc cgccttcgca
    30601 ggctcgtcac ccacacctgt gacggacaga aacccggatt cgctctggat tagaaggtcg
    30661 tacgctttct tgtcacgata gggcccatcg tcaccgtacc cggagatatc gcaaacgata
    30721 agcccgggaa aacgcgcgtg cagcgcctcg aacgacagac ccagccgcgc cgcggcgccc
    30781 ggcgccaggt tctgcaccag cacgtccgcc ttttccagca aacgccccag aatgtcggcc
    30841 gcctcgggct gcttgatgtc gagcgccagg ctttccttgg agcggttgac ccagacgaaa
    30901 tgcgatgaca tgcctcgcgc ccgttcgtcg tagccccggg cgaaatcgcc gcttccgggc
    30961 cgttcgatct tgatgacgcg cgcgccgaga tcggcgagtt ggcgtgtgca gaaaggcgcc
    31021 gcgatggcgt gttctaacgt gatgacggtg atgtcctgca aaggcaacat gcttgtttcc
    31081 cggttcagaa cgagcgcggc atacccagca cgtgctgggc cacgtaggat aggatcagat
    31141 tggtggaaat gggcgccacc tggtagagcc gggtttcgcg gaacttgcgc tccacgtcat
    31201 actcgcaggc aaagccaaag cccccgtgaa cctgcagaca cgcattggcc gcttcccacg
    31261 acgcatcggc cgccagcatc ttggccatat tggcctgcgc accacaatcc tggtgcgagt
    31321 cgaacagcgc gcaggcctgg taacgcatga gactggccgc ctcgacgttg acataggcct
    31381 tggcgatggg aaactgcaca ccctggttct ggccgatggg acgaccgaac acgattcgct
    31441 cactgacata tttgcgggcg cgatcgacga accaatagcc atcgcctatg cactccgcgg
    31501 cgatcagcgt gcgctcggcg ttcagcccgt ccagaatgta cttgaagccc cggccttcct
    31561 ctccgatgag gttctcttcg gggatctcca gatcctcgaa gaacagttcg ttggtctcgt
    31621 gattgaccat gttcaggatg ggccgcaccg tgaggccgtt gccgatggcc ttgcgcagat
    31681 ccaccatgaa gatcgacatg ccttcggatt tccgcttcac ctgttccagc ggcgtggtgc
    31741 gcgccagcag caccatgagg tcagaatgct gcacgcggga gatccacact ttctggccat
    31801 tgaccacata acgaccatcc ttcttgacgg cgacggtctt gatgcgggtg gtgtccgtgc
    31861 cggtggtggg ctcggtgacc gccatggact gcagccgcag gtcgccgctg gcgatcttgg
    31921 gcagccattc ccgcttctgg gcttcggagc cgtgtcgcag caccgtgccc atgttgtaca
    31981 tctggccatg gcaggcgccg gaatttccgc cggcacggtt gatctcttcc atgatgaccg
    32041 acgcttccgt caggcccagc ccggaaccgc catactcttg aggaatgagg gccgccagcc
    32101 accccgcctt ggtcagggca tcgacgaatt cggccgggta gccccgttgt tcatcgatgc
    32161 gacgaaaata ttcgtcgttg aattgtgaac acaggtcgcg gaccgcgtca cggatatcct
    32221 gatattgtcg tggtgcctca aacattcatt acctttcatt catggaaatc gacacgggcg
    32281 gcaacgcaaa gccacagccg caagcaccgt agagtgaagg aatcaagcag cgctggcaat
    32341 tcggatttgc caaatgtagg cttagccacg tcttaacctg gccacgtggc ggccgattga
    32401 cctggaacct ggttcagctt gggccaactc gggttccgcc atcttcggca cgcttcagcc
    32461 gattcaacgc aggaacagag ccggcgccct tcgactgcgg gcttcgtgcc gccgggtatc
    32521 ccggtatggc gctatttccc cgcgtcctga accagcagat cgaccagttc ctgggcgaaa
    32581 gccggcagct tcccagcctc ctgcacgcag atgtgcagct tgcgcagcgc ccagtcgtcg
    32641 gtgagcgcca tgatcttcag tggcatggtt ttctcgtagc ggcgcgcggc ggattcggga
    32701 atgacgccta tgccgacctg ggcggcgatc aggcgacacg aggcctcgaa gtttccgacc
    32761 tcgacccgca tgcgcaggat gcggccgctg tcctcggccg ccttcagcag aaaagaatga
    32821 atggcgctgg actcaaacag gccgatgaaa tcgtaatcca gcgtcctctc gaagctgtgc
    32881 tcggcgtgtc gggccaaagg gtggtccttg tgggtgacca gcaccagccg atcgacgcga
    32941 taaggcagaa actcgacccc ggccccgccg ctgccctggg cggtgatgcc gatgtcggcc
    33001 accccttcgg cgaccgcctt caccacctgg tagctcagtc gttcgcgcaa ttcgatgttg
    33061 acatcggggt gatccaccag atagcgctcc agcgcggcgg gaatgaattc gttcatggcg
    33121 gtggtgttgg cgtagacccg cacccggccc ttcaccccct tggcgtactc ctgaatgtcg
    33181 ccacgcaggt gctccagttg gcgcagcacg atgcgcgcat gccgcacgaa cgcctcgccg
    33241 gatggcgtga gcgtcacacc ctggctggtg cgatacagca gcgcggtgcc caggtaggtc
    33301 tcgaggttct tgacccggtt gctggccgcc ggcagggaca gaaaggtgta ctccgcccct
    33361 ttcgtcaggc tcatcgcgcc cgccacgttg accatcaact gcatgtcggt caggtcgaaa
    33421 tgcattgcca taacgcggct ttctcctccg aaattgtgaa gccatcctta agcaaacaag
    33481 aattggcaaa tcgatcgcct tcgcgcacag tcatggctca tacgaaccaa tcggccaaca
    33541 tcaccgggcc ggacgacttt ccattgcagg agccctcatg caacacccca gcgcggaact
    33601 ttcccggttc gccgccgaac tgcgattcga cgacattccc gagcccgtcc tgcgacgcgc
    33661 cgaagacctg ctcttggact gcctggcgtc cattctagcc ggatcggaca gccgcgccgt
    33721 gaaaactctg aacggctacg cccaggcaat ggggcccgac catggtcccg ccgagaacat
    33781 cgtgggcagg cgcggcacca gcccgctgtt cgccgccatg gtcaacgcgg ccgcgtcgca
    33841 tatggtcgaa caggacgacg tgcacaacgg gtcggtgttc catcctgcgg ccgtggtgtt
    33901 cccgcccgcc atggccaccg cgcaaacgct ggaacgatcc ggaaaagacc tgctggtcgc
    33961 ggccgtcgcc ggctacgaaa ccggcatccg catcggtgaa ttcctgggac gctcgcacta
    34021 cacagtcttc cacaccacgg gcacggccgg cacgctggct tccgccatgg cgaccgggcg
    34081 gctgctgggg ctcgaccccg gccgcatgtt gcacgctctg gggtcggccg gcacgcaggc
    34141 cgccggtttg tgggaattcc tgcgcgatgg cgccgactcc aagcaactgc acacggcaaa
    34201 agccgcgggt gacggcctga tggccgcctg gctcgcccgc gacggattca ccggcgccgc
    34261 gcacatcctc gaaggcccgc aaggcctggc cgccggaatg tcccaggacg ccgatcccgc
    34321 ccgcctgacc gacagactgg gtacccgctg ggcgttgctg gaaacctcat tcaaatacca
    34381 cgcctcgtgc cggcacaccc atccggccgc cgacgcgcta ttggccatcg ttcgcgacaa
    34441 ccacctgcgc atcgacgata tcgcctcggt ggtcgcgcat gtgcaccagg gtgccatcaa
    34501 cgtgctgggc cccgtcgtcg atccggccac cgtgcaccag tcgaaattct caatgggcac
    34561 ggtgctgggc ttgatcgcca aacaccagcg cgccggtctg ccggaattcg acaacgccct
    34621 gtccgacccc gacatcattg aattccgtca acgggtatcc atgcggctcg accacgaggt
    34681 cgacgccgcc taccccaaac gttggctagg taaggtgacg gtcgtcacaa aatcaggcaa
    34741 gacactcgaa ggccgagtcg acacccccaa gggcgacccc gacaacacgc tcactcgccc
    34801 cgaaatcgaa gagaaagcca tccgcctggg gggttaccac aacgccgcca cgccagccga
    34861 aatccaacgg ctcatcaccc gtgtatggaa cctggccgac accccgcgcg tcggaacctt
    34921 cctcggcaaa ggtgaagaat gaccacccga gaaccctacg gcggccacag aacgtccacg
    34981 catgcaattg ccgcaagctg tctatcggag tcaggggtgg gcccggcgta aggcgtgcgc
    35041 cggatctggc ttggccaggc gggggcagtc gtcgggcgct gtgtgagtaa aacggccagt
    35101 cctacagact ggccgttttg cgagtttcgc ccgacgcccg cctggacgag acagatccgg
    35161 atgaatcacg ccgtgccggg cctgcccctg gctccgatag acagcgcctt caagtaccga
    35221 cagcgtattc aaacacccgc agcggctcca aacacccaca gcatcgaatc ccgccagcaa
    35281 tcaaacccaa accgcgagag cagtttaggc gcaaatattg accggatttc cacctgataa
    35341 gcctgatatc ggtcatacga aatcttgaac gctgtacaca cctgaaccta acatgcgcta
    35401 cccagcccga gccacggtcc gccgggcacg ctcgatgaca ggcagatcga ccatcttgcc
    35461 atcgagcttg aacgcataag agccactggc atcagcctgc tccacgacac gccgcgccca
    35521 ggccagttcc tgacttgacg gcatgaaagc acgatgcacg acctcgacct gggccggatg
    35581 aatgcacagc atgccgccaa accccatgtc gcaggcctgg ccagccgcca ccaccaaccc
    35641 ttcggtatcg gcgaaatccg gatggatggt atcaatgggt gaattcaacc catggacccg
    35701 gctttgcacc aggacgtgat agcgcacctg gttcaacagc atcgcggccg ccgacgtatc
    35761 gggcgtgatc cccagatcaa gcatgagatc cagtgcgccg aacgtcagtc ggctgacact
    35821 gggcgcaccg gcgatcgcat ccagagcctg cagcccccgc gcgctttcga cgatggggaa
    35881 taccggcttg cccgctgcgg acgcatcacc cacctgctcc gcggactcgg ccttgggcag
    35941 caagatgcca accacgttcg gcaattcgcc acagacgccc agatcagcat tgaaccacgg
    36001 cgccccggcg tcgttgaccc gaacgagaaa cgccgtgtga gggttgtccg ccgcgaattg
    36061 gccgagattc ttgcgcgcga tgtctttcgc gtcacgttcg acagcgtctt cgagatcgat
    36121 gatgaccgcg tccgcaccac tcgccacggc tttggcgaaa cgttccgggc gcgtccccgg
    36181 gacgaacagc gctgtacgca gcattttgaa tgtatccatg atcagaatcc gttcctgcaa
    36241 atccggcacc aacaaatagg cacccacatt acccttcgaa tcggacctcg gcgcgatgcg
    36301 ccagtgtgcc gtcctgctcg gcccagagca tcgcaccctc gcgcgattcg aaccgccccg
    36361 cgacctgaaa ggcgcggggc gcgatcaggg gacgcaaccc gcgataggaa aactcgctga
    36421 ccaccttgcc gggattggcg cgcgtgaacg cgcggcccat caacgtggcg atgagcggcc
    36481 cgtgcaccac cagtccggga tagccctcga cctcgcggac atacggctca tcgtaatgga
    36541 tgcgatggcc attgaacgtc accgccgaat aacggaacag catcacggaa gtcggctgta
    36601 tggtgtcggc ccattccggt tccggcgccg gctgcgtgcc ggacaagatc ggcggggagg
    36661 gttcgcgata gacgatgtcc tgctcctcgg cggtgcagag catgccgtcc tggaaatagt
    36721 cgtgtttcac ggtcacgaac aacagctttc cggtgcggcc ttctttctcg gtgacatcgg
    36781 caatggtcga catccgctca ctcgccacgc caaccagcag cgggcgcagg aatttcagac
    36841 gcccgcccgc ccacatgcga ttgcggtcgt ctgcgggcgg cagaaacccg ccgcgccgag
    36901 gatgaccgtc gccgcccaac tgcgcatagg gttgggcgtt gatgaaaaac gcccagtgcc
    36961 acaacagcgg caacgcctcg ccgtgcgccg gcgccgcgtc acccaggctg agcgccacgc
    37021 gttcgacatg tccgatgtcg aggttgtcgg tgcacgattc ggtgcggccg atccaggatg
    37081 ccggatcaga agtagtcatg gttgtctccc ggcgcggtgc gccttgaata cgcctgtcag
    37141 gcaggcacga ggttgatgcg ggcgatgcgt tccttccatt cgcgggggcc agtctcgtgc
    37201 acggacgtgc cgctggaatc aaccgccacc gtgaccggca tgtccttcac atcgaactcg
    37261 tagatggctt ccatgcccag gtcctcgaaa cccaccacgc gcgcatggcg gatagccttg
    37321 gataccaggt aggccgaccc gccgactgcc atcaggtagg cggaaccatg gcgccggatg
    37381 gactcgatcg cgaccgggcc gcgctcggcc ttgccgatca tggcgatcag gccggtgcgt
    37441 tccagcatca tgtcggtgaa tttgtccatg cgcgtagatg tggtggggcc ggcggggccc
    37501 acgacctcgt cacgcaccgg atcgaccggc ccgacgtaat agatgacccg attgctgaag
    37561 tccaccggca acggttcgcc gcggtcgagc atttcctgaa tgcgcttgtg cgcggcatct
    37621 cgtcccgtga gcatcttgcc ggagagcagc agagtctggc ccggcttcca actcgcgact
    37681 tcctcgcgcg tgagggtgtc gaggttgact tgctgcgact tgttgtagtc tggcgcccat
    37741 ttgacgtcgg gccagacatc cagcgagggc ggggtgagca cagcgggccc tgaaccgtcg
    37801 agcgtgaaat gcacgtggcg ggtcgcggca caattgggga tcatggcaac gggcttggac
    37861 gcggcgtgcg tggggaacgt cttgatcttg acgtcgagaa ccgtggtgag gccgcccagc
    37921 ccctgcgcgc cgatgcccag cgcgttgacc ttctcgtaca gggcgatacg cagctcttcg
    37981 agcttgttcg agggcccgcg ggcaagcagt tcgtacatgt cgatgtcttc catcaacgac
    38041 tgcttggcca tgagcatggc tttttccgcg gtacccccca cgccaatgcc cagcatgccc
    38101 ggcggacacc agcccgcccc catcttgggc acggtcttca gcacccagtc gaccagggag
    38161 tcgctgggat tgagcatttc gaacttggac ttgttctcgg agccaccgcc tttggccgcc
    38221 acctggacgt cgaccgtgtc gcccggcacg agctcgacgt tgacgataca gggtgtgttg
    38281 tcgcgcgtgt tcttgcgcgt gaacagtgga tcatcgagaa tggaagcacg caatggattg
    38341 tcggggttcg tgtacccgcg gcgcacccct tcgtcgcaaa cgtcctgcag gctttgcttc
    38401 gtatcgaagc gaacattcat gcccaccctc aggaagacgt tcaccacgcc tgtatcctgg
    38461 cagatggggc ggcgcccttc ggcgcacatg cgcgagttcg tcagtatttg cgcgatggcg
    38521 tcgcgcgcgg cgggattctg ctcgcgctcg taggctcgcg ccaagtgctg gatgtagtcg
    38581 gagggatggt agtagctgat gaactgaacc gcgtcggcga cggactggat cagatcgctt
    38641 tctgtgatgg tggtcgtcat gatgtggatt tccgaagaat actatgaagc caaccgctgg
    38701 cactgcaacc aacggggcac tgatgcctgc cgaaccaggg cctgttcaca ttgaacgggt
    38761 tcctagcggc ctttccatat gggtttgcgc ttctcggcaa aggccgtggc cccttcgcgc
    38821 gcgtcttccg aagtgaagat atgcgcgaca cgcggccgct gcaggtcgaa catgtcggcc
    38881 tggcgccagt ctggggattc cgtcaggatg cgtttggcgg tgcgcaccgc cagcgggccg
    38941 ttctcggcga tggcgcgcgc catctcgagc gccgcgtcca gggccttgcc tggttcgacc
    39001 aggcggttga ccaatccgta ctggcaggcc cgctgtgccg gaaacatggc cccggtcagc
    39061 acgatctcca tggcgatgtg cgggggaacg cgccgaggca ggcgcagcat gccgccagag
    39121 cccggcacca ggccgcgctt gacctccggc aagccgaaac tggcgttctc cgccgccacg
    39181 atcaggtcgc aggccagcgc gatctcgcaa ccgcccgcca gggcgtagcc ttcgacggcc
    39241 gcgatcagcg gtttggccgg cggcctttcg caaatgcccg cgaacccccg cccgtcgacg
    39301 taggggcgcg tgccggactg cgcaaacgcc ttgagatcca tgccggagca gaacgaattg
    39361 ccggcgccgg tgagcacacc cagcgcgatg ccgtcgtcgc catcgagccg gtcgagagcg
    39421 ttggccaacc cgcgcgccac ctcgacattg accgcgttgc gcgcctcggg ccggttgagt
    39481 gtgatgacca ggatgcggtc gaccacatcc accttgacca aatcggacat tgcacatccc
    39541 ctgtagatcg tcgcaccccg gaaccatgcc acggggcgcc ttgaaaaggc gaagcggccc
    39601 cggccacggg ggccaaggcg ctgccagcgt tattatcaca cgcccttcgg gttccgcgct
    39661 gtcgcacgtt cgtgctctcc ggttggaacc agttaaaatc agggactttg cagtccattg
    39721 cccacgggcc cacctcatgc tcacctttca gcagatcatc ctgacgctcc agcactactg
    39781 ggacaaacag ggctgcgcgc ttctgcagcc ctacgacatg gaagtcggcg cgggtacgtc
    39841 gcacaccgcc acttttcttc gcgccattgg ccctgaaccc tggcgcgccg cgtacgtgca
    39901 accgtcacgc cgccccaagg acgggcgcta cggcgacaat cccaaccgca tgcagcacta
    39961 ctaccagttc caggtcgtgc tcaaacccgc tccgcccgag atcctcgatc tctacatcgg
    40021 ttcgctcatg gcgctgggca tccaccccga tcagcatgac atccgattcg tggaggacga
    40081 ctgggaaaac cccacgctgg gcgcatgggg gctgggctgg gaagtttggc tcaatggcat
    40141 ggaagtcacc caattcacgt atttccagca ggtgggcggg ctggactgcc taccgaccac
    40201 gggcgaaatc acctacggtc tcgaacgcct ggccatgtac ctgcagaacg tcgaaagcgt
    40261 ctacgacctg gtctggaccg aaggtcggaa cgggcaccgt gtcctgtacc gcgacgtgtt
    40321 ccaccagaac gaagtcgagc aatccaccta taacttcgaa ctcgccaaca ccaccatgct
    40381 gttctcccat ttcggcgact acgaaaccga agccaagcgc ctgatcgagg ccgaactggc
    40441 gctaccggcc tacgaggccg cgctcaaggc cgcgcacaca ttcaacctgc tggatgcgcg
    40501 cggcgccatc agcgtcaccg agcgcgccgc ctacatcggc cgcatccgca acctgtcccg
    40561 ggcggtcgcc caggggtacc acgattcccg cgaacggctg ggctttccca tgctggcctc
    40621 ggcgccagac gtggaggcca cgcgatgagt caacccggca ccacccactc acgccccctg
    40681 ctggtcgaac tgctcaccga ggaacttccc ccgaaggcgt tgcaggaact ggggcgcgcc
    40741 ttcgccaacg gcatccatgc caccctggtg aaacaagggc tgctcgacgc ggatagccac
    40801 atcgtcgatt tctccacgcc acgccggctc gctgtccgtc tcgaacaggt gcgcgcccag
    40861 gcccccgagc aggcattccg tgaaaaactc atgcccgccg ccgtcggcct caccgccgat
    40921 ggccagccca gccccgccct gctgaagaag ctggccgcca aagggctggc acaccttggc
    40981 ccgaacgacc ttcagaccga atccgatggc aagcaagact atctgttcgc cgccgggacc
    41041 actgccggca tccgtctggc cgatggcctg caagaggctt tggatcacgc catcgcccac
    41101 ctgcccatcc ccaagttcat gcgctaccag ttggccgatg gcgtgaccag cgtgcggttc
    41161 gtgcgccccg cgcacagcct gcttgcgctg tggggcgacc agatcgtgcc ggtgggcgcg
    41221 ctggggctga ccgcgtcgaa cgcgacgcaa gggcatcgct tcatgtccga aggctccctg
    41281 accatcacct cggccggttc ctacgaacag caactgctgg gcgagggcaa ggtgatcgcg
    41341 tccttcaccg agcgccgggc ggacatcgac caacaacttc aggcgcaggc gcaacgcctg
    41401 ggggcaagtc tgggcgatga cccggaagtg tccaggctgc tggacgaggt cacggcgctg
    41461 gtcgagcatc ccaccgtcta tgtcggggaa ttcgatccgg aattcctggg tgtgccgccg
    41521 gaatgtctga tcctgaccat gcgcttgaat caaaagtatt ttccgttgtt cgaacccgac
    41581 accggcaaat tgacgcaccg gttcctgatc gtcagcaata tgcgcgtcga tgacccctcg
    41641 aacatcatcg agggcaacca gcgggtcgtc cggccacgtc tggccgacgc gcagttcttt
    41701 ttccacaccg accgcaagac ccccctgtcg gcgcgtgtcg atcaactggc caacagcgtc
    41761 taccacaaca agctgggctc gcaactcgat cgcgtgcgtc gcattcaggc catcgctgga
    41821 cacctgtcgc aacgcctggg tgccaacacc gggcgcgccg agcgcgcggc gtggctggcc
    41881 aaggccgatc tgaactccag catggtcggc gagttccccg aactgcaagg catcatgggc
    41941 gcctactacg cccaggcgga gggggagccg gatgatgtgg tcgccgccat tcgcggccaa
    42001 tatcgaaacc ggatcgaagt gccgatcacc gctgacagtc tggtgcccgc cgtgctgttc
    42061 atcgcggagc gcgtcgaaac cctgatcggc atctggggca taggcctggt ccccaccggc
    42121 gagcgcgacc cgtttggcct gcgccgcgcg gcgttgggcc tgatcagcgc cttcgaacaa
    42181 ctgcgggccg gcggctactt gaacgacacc gccacccctg gcctcgatct gccaggcctg
    42241 ctggccttcg cgacgaatac gttcaccggc cacgcgctga atccgtccac acccggcgag
    42301 gtcgaagcct tcatccacga acgctaccgc aaccaactgg ccgatcacga ccgccacgtc
    42361 gtcgatgccg tgctggccct cacgccgccg ttgcagcagg ttctggaccg cgtgcgggcg
    42421 tgcaccgatt tcgcccagcg gcccgaagcc gccagcctgg cggccgccaa caaacgcatc
    42481 accaaccttc tcaagaaggc ctccgatacg cagcgccggt tcgaccccac cctcatgcgc
    42541 gaacccgcgg aaaaagccct cgcggccgtg attgctgaac ttgagcctca ggcgcggtcc
    42601 gccttcgccc agggggattt cgtggcatgc atggccacca tggcccgggc gcgtgatgcc
    42661 gtggatgcgt tcttcaacga catcatggtg atggacgaag atccagccat ccgggccaac
    42721 cgtctggcac tgctcgccag cctgcatgcg gtgatgaacc tggttgccga catttcccga
    42781 ctgacccagt gaaactagcc attctcgatc gcgacggcgt catcaatcag gacagcgacg
    42841 ccttcatcaa aagccctgac gaatggattc ccgtgccagg aagccttgaa gccatcgcac
    42901 gcctatcacg cgcgggctgg cacgtcgtca tcgccagcaa ccaatcgggc atcgcgcgcg
    42961 ggctgttcac catggccacg ctcaccgcca tacacgccaa gatgcgccgt gagttggcca
    43021 aggcgggcgg cgccatcgac gccctgttcg tctgtccaca tggcccggat gacgattgcg
    43081 cgtgccgcaa gcccaggcca ggcatgtttc gggatatcgc gcgccgttac gacatcgcgc
    43141 tcgaaaccgt gcccgccgtg ggggattccc tgcgtgatct gcaggcagcc tcacaagctg
    43201 gctgcacgcc gtggctggta ctgactggta aaggtcaagc cacctggaat gcgggtgacc
    43261 ttcccgctgg cacgcaggtg cgcaacgatc tggccgaggt cgtggacacc tggttgtccg
    43321 ggggctgagc atcatggcgg ccctgcgcgc gacactctat ctcctgtttc tgaccatcac
    43381 ggtcattccg tacgcgctgg cttgcattct gtggtcacca ctgcccctgc gatggcgcta
    43441 tcgcctcacc gccggctggc cccgcttggc catctggggc gccaagacac tgcttggcat
    43501 acgctggcgc ataaaaggcg ccgagaatct gcccgatggg ccggcagtcc tgctatccaa
    43561 gcatcagtcc gcgtgggaaa ccctgttttt ccccgctcac atgccacgcc aggtgtgctt
    43621 cgtctataaa agagaactgc atcgaatccc gttcttcggg tgggggctgg ccctgctgcg
    43681 catgattccc atcgatcgag ccaaggggcg cgacgcgttc gagcaggttg tgcgtgtcgg
    43741 ccggctgcga ctggacgagg gacgatggcc cgtgctgttt ccagaaggca cccgagtggc
    43801 gccggggaaa atggggcgct acaaactcgg cggcgcattg ctggccgccc gcaccggcgc
    43861 ctgcatcatc cccatcgccc acaatgctgg ggagtgctgg ccgcgcaacg cgttcatcaa
    43921 gaagccgggg ctcatcaccg tctccatcgg cccggcgatc gcgaccgatg gccaggacgc
    43981 cgcgacgctc aacagaaaag tccaggaatg gatagaaggg gagatgcggg agttgaaccc
    44041 cgaaaggtat gaaccatcct gatcagttga ccctcccctt cgatttaccc gacacacctg
    44101 gagcgccgcc cgcccttcag gttccacggg cgaccgatat cgcccccgcg cccctgactt
    44161 cctgccctcc gcttctgccg cagggcgcca agtggcgtga agtgttgtct ggcgggcggc
    44221 ccatcggctt cgtcctgcaa cgctccaagc gcaagaccat cggcctcgtc gtcggtgacg
    44281 acgggcttcg catcacggct cccaaatggg tcaccttgcg tcaaatcgac gacacggtgc
    44341 tcgagaaatc gtcctggata ctcgacaagc tcgcacagca tcaggcgcgc caggagcaac
    44401 tggccctggc cgacacccag tggcgcgatg gcggtcattt cccgtacctg ggcacgcaga
    44461 tcgtgctcgt gctggggagc cacgatggac gagcggccta tcagggtgat gtgatggcac
    44521 cccagcccgg cgacacgctg cgcctgggcc tgcccggcga cgccgatcgg aacagggtgc
    44581 gggacagcgc gcagagctgg ctgcagcaac gcgcgcgggc atggttcggt caacgcctgg
    44641 aacatttcca gaaggtgagc ggacggacca tcaatcgctg gcgcctgtct tcggcggcga
    44701 cgcgctgggg gtcgtgcaac agcgacggca atatcatgct caactggcgt ctgatccact
    44761 tcgcgcctcc catcatcgac tacgtcatcg cgcacgaaat cgcgcatctg cgcgaaatga
    44821 atcatggcgc cgatttctgg cgcgaagtgg gccgcatcct tcccgggttc gaggctgcgc
    44881 gcaataccct caggcagcat gatccggcca cgctgccttt gctctagggc ttccggggag
    44941 aaccccgtgg gccgccggcg acaaactcaa caaggagatt tcaatgcgtc tgctccatac
    45001 catgcttcgc gtgggcgacc tggatcgctc gctcgcgttc tacacgaacg tcctgggaat
    45061 gaaggtgctg cgccgcaatg attacccaga agggaaattc acgctggcat tcgtcggata
    45121 ccagaacgaa tccgaggggg ccgtgatcga gctcacccac aactgggaca cgtccagcta
    45181 tgacctgggc agcggttacg ggcacatcgc gctggaagtg gaggacgcgt acgcggcctg
    45241 tgccacgatc aaggaaaaag gcggccgcgt cacgcgcgag gcgggtccga tgaagcatgg
    45301 caccacggtc atcgcgttcg tcgaggatcc ggatggctac aagatcgaac tgatccagca
    45361 taagcggtag tggatttttt ggttcgggtg ctggtggggt tgggcgctgt tggtgtctga
    45421 aagcgctgtc tgtcggagag ggcgttgagg tatctgaaga cgctgtctat caggaccagg
    45481 gcgggtccgg cacggcgtgc ttcatccgga tctgtctcgt ccaggcgggc gtcgggcgaa
    45541 actcgcggcg gggccagtcc aagggactgt ccccgccgct cagacaccgc ccgacgacta
    45601 cccccgcctt cccgagccag atccggcgca cgccttacgc cgggcccgcc ctggtcccga
    45661 tagacagctc gcgatgaggg tatgcggggg gaccacaacc ctagtcggaa caggtgggtg
    45721 gcactacctg tggcggggca gatctgccgc cggaaaatcg tcgacctcga tgacggcggc
    45781 cacggccgcc tcggcgtctt caagcagctt cgcctgcgca tcgtcgatgg cgcccttttc
    45841 gtgggcgatg cgccagtctt gcaagccgct gtgcctgagg cgttcgagca ggggctgggc
    45901 ctgcgtcacc agttcgaacg cctgctccag ttgctcgaca ccggcgtggc tgtgcccgcc
    45961 ccacacatcc ccgaccaggc gattgcgtgt gccagagggc tccagcagaa tccgggcgca
    46021 ggacgtcagc acatcatccg aaggcggctt tgcgcgccgg aagaacggca gcaccacgac
    46081 gcgcaacgcc actgacatgg cccgcgacgg atagttgcgc agcacgtcgt cgaggcgttc
    46141 ttcgatggtg gcgaagccct gttcgacgca gcaccgcacg agtggaagat cgggctcgtg
    46201 ccgcccttcg tcgagccagc gcttgagcac cgccgacaac agatagagct cggccagagc
    46261 atcgcccagc cgcgccgaca gcatttcccg ccgcttcaac gccccgccaa gggaaaacag
    46321 ggtggcctcg gacaccaggg cgaatgcggc ggcgtagcga cccaggcgtc gatagtaggg
    46381 ggcggtcggt cccgcctcgg cgggcggcgt gccggaaggc aagccaaacc aggcgctgga
    46441 caatgcgcgg aatccgttgc gcacggaatg cccgagatgc ttccagacga tatgatcgaa
    46501 cgcctccagg ccagcgtcct ggtcgctatc ggccagcgcc ctcacctcgt ccatgagata
    46561 cggatgtgcg cggatcgcgc cctggccgaa cacgatcagg ctgcgcgtga ggatgttggc
    46621 gccttcgaca gtgatgccca ctggcaccgc ccgatagagg ccacccagat aattcctggg
    46681 tccgtcgatc acagcctttc cgccatggat gtccatggcg tcgttgacgg actgcctcat
    46741 gcgctcggtg gcatgaagct tcatgatggc cgatgccacc gccggcttgt gccccatgtc
    46801 caggcccgcg caggtgaagc ggcgcgcggc ttcgaccaga taggcgttcg ccgcgagccg
    46861 gccgagtttt tcctgcaccc cttcaaactg ccccacgggt atgccgaact gtgtgcgcac
    46921 cctggcgtag caacccgcgg tatgcgcggc gaacacacac gcgcccgccg acaaggatgg
    46981 cagtgaaatg ccacgacccg ccgccagggc ggccatcagc atctgccagc cctgtcccgc
    47041 cctctccaca ccgccaatga gagcgtcgat gggaacgaac acgtcatggc cctggttcgg
    47101 gccgttctgg aacgcctgca atgccggcaa atgccggcgc ccgatcgaga tgccagggag
    47161 gttcgtgggc accagcgccg cggaaatgcc gatatcggag gtatcgccga tcaggtgatc
    47221 cggatcggac aacttgaatg ccagccccag caccgtggcc acggggctga gggtgatgta
    47281 gcgcttgcgc cagttcaggc gtatgcccag tgtgtccacg ccgtccacca tctgccggca
    47341 gaccacgccc gtgtctgtca tggacgcggc gtcggatccg gcctgcgggc tggtcaggcc
    47401 gaagcacggg atttcccgtc cgtcggccag acgaggcaac caatggccgc gctgcgcctc
    47461 ggtgccgaac tgcatcagga gttcgcctgg cccaagggag ttgggcacca tgaccgtcac
    47521 gccggccgtg accgagcaca acgacacacg acgcacgatt tccgaatgcg cgcgtgccga
    47581 gaaacccagg ccgccatact ccacgggaat gatcattccg aagaatttat gccgcttcat
    47641 gaacgcccat gcctcgggcg gaagatcgta ccgactccag gagatatccc aatcgtcaag
    47701 catgccgcac agctcttcca ccgggccgtc cagaaacacc tgctcttcgg gcgacaatcc
    47761 ggatgccttg attgcgccga aggcctgcca atcggggtgt ccggagaata actgggcttc
    47821 ccaccagaca tcgcccgcct cgatcgcctg ctgttctgtg ggcgatagcc gcggcacggc
    47881 cgagcgggcc atgcggtaga ggggttcagt cagccattga cgacgccagc cattccagtt
    47941 catcccatct cccttgtcga acggggcaca gttacgtacc attaccatca aggtgaagcc
    48001 accgtcaata gcgggccgcg cccggcctgt ccctcgcggc gcatcgcgcg ccagcacagc
    48061 caccacccaa cccgtcatct tctatgctca acacgctctg gctggcattt ttcgtgatcg
    48121 cttcgatttc ggggctgtat cactgggtgg tgctgcacga tcccgcggtg ttctcgcgca
    48181 tcgtcctgag cctgttcgac atggccgacc tgtcggtcaa gatcatgatc ctgctgttcg
    48241 gcacgctgac tctgtggctg ggcttcctgc gcatcgccga agcggcgggt ctggtggaat
    48301 ccctggcacg cctgctgacg ccgttgtttc gacggctcat gccaggcgta cccgccggcc
    48361 atccgtccat cgggctcatc accctgaatt tcgtcgccaa cgcgctgggg ctcgacaatg
    48421 cggcgacccc catcgggctg cgcgccatgc attccctgca gtcgctcaac ccggacccca
    48481 agacggccac caacgcccag atactttttc tggtgctgaa ctcgtcatcg ctgacgctgc
    48541 tgcccgtcac catcttcatg tatcgcgcgc aacagggcgc acccgatccg acgctggtgt
    48601 tcctgcccat cctgctggcc acgtcggcat cttcgctggt gggcttgctg acagtcgcct
    48661 tcatgcagcg actcaagctg cacgatccgg tggtgctggc ctggctgggc ggtgtggcga
    48721 tcgtgctggg cggcttcatg gccactctgg ccaccatgac atccgctgcc atcagcacct
    48781 tgtcatcgct catgggaaac ctgatgctct tcagcatcat catgatcttc ctgctggtgg
    48841 gatggcgcag aaaggtgcct gtctacgaat ccttcgtcga cggcgccaag gaaggctttg
    48901 acatagcacg caacctgcta ccttacctgg tcgccatgct gtgcgccatc ggcgccctgc
    48961 gggcctccgg cgccctggac gccgcgctcg acgccatacg ctggtgcgtg cactcgctgg
    49021 gcctggacac ccgtttcatt ccggcgctgc ccaccgcgct ggtgaagccg ttttcaggca
    49081 gcgcggcgcg cgccatgctg atcgaaacca tgaagcacta cggggtggac agcttccccg
    49141 ccctgcttgc cgccaccgtg caggggagca cggaaaccac gttttacgtg ctggccgtct
    49201 atttcggcgc ggtgggtgtg cagcgcgcgc gccacgcggt cggttgcgcg ctggccgccg
    49261 acatcgccgg cgtgctggcc tccatagcga tttgttattg gttcttcgga tagcacgatt
    49321 cccaagcgca acccagccct atcgggtaga ggccgacggg gctaggggga tccgacacgc
    49381 gacaccacac aggagctcaa gcggcctgtg ggcgcaccgc gcgcgaggat gacgtggccg
    49441 gcagcaacag tacccccacg aggcctatcg cgacgcttac catcacatag tagatgggcg
    49501 ccatgttgct gcccgtggcc ttcaccagcc aggtattgaa tagtggcgcc aacccgccaa
    49561 acaccagtac cccgacgtta tacgtgattg atgcaccagt ggaacgaaca cccaccggaa
    49621 aaacctcggt caaggtaatg ggcgtcggcc cccagaagaa actcatgaag aacgagatca
    49681 tcacctgggc aagcaacagg gattcaaagg atggcgactg cacgaagtga cgaaaaagaa
    49741 agtaccccgc tatcccatag gcaatgatgc cgggcaggaa gaccgccttg cggccaaatc
    49801 gatccgacag gtatccagcc agagggcagc acaccagcaa taccgcagta cccacgaaag
    49861 tcgccagatt cacgtctgcg ataggcatgc ccaattgcct cttcgcgaat atgggcatga
    49921 agaaaacgaa aacatacgcg gagacggtgc ccaccacgca aatgcacagc gtggccagca
    49981 tctcgcgcgg gtggtctctg aaaagattgg cgagcgaggc acgaatgctt tcgcgcgcag
    50041 cctgcggccg gttgcgcaca taggcaagga attccggcga ttcgtcgaga tgggcgcgta
    50101 taacccaacc cactggaccg accagaatgc caaacagaaa cggaatgcgc caaccccagg
    50161 catccagggc ttggggcgtc agatgcgcag tcaacaaata ggccgacagc gcgccacagg
    50221 agaacgccaa cgcctgagag cacatctgga aactaccata gaagcctcgg cgatgcgcag
    50281 gcgcgaattc aatcagcatg gtggtggccc cgctgaactc gcctccagtg gaaaacccct
    50341 gaatcaggcg ggccagcacc atcagcagcg gcgccgccac gccaatggcc tgataggtgg
    50401 gcaagacacc catcattccc gtgcccacag ccatcaggac tatcgtcagt gacaaggcct
    50461 tttttcgtcc aacccgatcg ccatacagac ccagcaacac accgcccaaa ggcctcatgg
    50521 caaagcctac tccgaacgtc gcaagcgtca gcagcaatga cgcaaagtcg ctatgggcag
    50581 gaaaatacag acgagaaata acgacggcga acaatccgaa aatggcgaaa tcgaaccatt
    50641 ccagcgcatt gcctatcacc gacgccacaa ccacgcgccg catcatacgc ggcgaaactt
    50701 ttgtgtcact catagtctcc tcctcgattt acgctcaggc cagacgctgg cgaacgattt
    50761 ccataaacac ttccgccccg gtggacaagc tgccgtcgtc aaaatcgaat tctgggtgat
    50821 gacaggtgcg gccgttgtgc cccagaaaaa aataagaacc agggcgctgt tccagcatga
    50881 aggcaaaatc ctccgacgcc atcaacggcg cggcttctac cacgttatcg gcgcccacga
    50941 ctcgaatcgc cgcctgacgc acgatctctg tttccgactc ggtgttgatc gtcgggggtg
    51001 aggacaacag atgctcgaac cggacttcac aaccgtacgt tgccgccacg ccgtcgcaaa
    51061 cctcgcgcag gcgatccaac accacctgct gggcttgccg ggacaacgtt cgcacggtgc
    51121 cttcaagttg tgcggtatcg gcaatcacgt tgatcgcaga gcctgcatga aactggcata
    51181 tgctcaagac cgccgtatcc agcggatcga cattgcgact caccacactt tgcagtgtct
    51241 gaaccaatga cgacccaacc aatagcggat ccaccgactt gtgcggcatg gcggcatgac
    51301 tgcccacacc acggatatga atacggaaca ggtcacatgc cgacaaaatg gccccacaac
    51361 gcacccccac cttgccagcc gggatgcaat tgttgttatg cagggcgtag atttcatcac
    51421 aaggatagcg gtcgaacaaa ccaagacgta tcagctccga gcctccacgc agcgtttctt
    51481 cggcgggttg aaaaataaag tgaaccgttc cacggaaatc gtcgtgcgcg gcgagatggc
    51541 gggccacccc cagaagaatg gctgtatgac cgtcatgacc acaaccatgg aatacattgt
    51601 catgaaccga ccggtattca aggtcggttt tctcttgcat gggtaacgca tccatgtcgg
    51661 cacgcagccc caaacagcgt cccgggccac gcttgccacg caacgtaccc accacggcgg
    51721 tcgtaccacc gaatcccgcc tcgacctcga tcccccaaga acccaacaac ccagcaactt
    51781 tgtccgccgt gcgatgttct ttgaaaccca attcgggatg ctgatgtatg tcacgacgcc
    51841 atccccgcat ctcatcggat agccgggaga tttctgtttc aaccaacatt gatgtgctcc
    51901 tgttcggtgc cagacgcagc ttgacggtat ttatccaggt gtcggcacag tttattagaa
    51961 tttttgtaga ttacttcatt agttgaattt ttcaactaat tgcggataat ggatttccct
    52021 aacccaagaa tcgcccacca tcagcgatgg tgactcaccc ccctcaccgg aatcgcgttg
    52081 aagatcagta caaaatgtcc tgaatcgacc gggaaaacgc cgtcaggcac cccttcagct
    52141 agaatgcatg ttgcaaatac aagtcacggg gccatgggca agaccaatac tgaaagcggc
    52201 tcgcacgacc cgggtttcac cagctacctg acgttcaaac tcgatctggt gaaatccgcc
    52261 atgctgcatc gagccaatca ggtttaccga cgcgaactgg ggctggatgt gcgttcattg
    52321 cgtctgctgc gggccatctg cgacacgccg ggcataaccg ccacgcggct gcgtgagatc
    52381 acgctagtgg aaaaaacctt gctatccaag gatctgactc agcttgtgag tcttaaactg
    52441 gtccgccgct ccatccaccc tgatgacgcc cgccaccacc agctctgggc gaccgccggt
    52501 gggaagcgca tacgcgcggc cagtgacgat cttggtcacg ccatggaaaa cgtcatgctg
    52561 tccatgctgt catccgaaga acacgcaacc ctgaatgaac tgctggggaa actgagccga
    52621 gaatttctcg cggaaaccgg ggatgtggac gatctgatag gcgtgttgaa cacacatagg
    52681 gcgaaagcca gccaacgaca aagcaaagac tgaaacgcag cggcccgtgt tttgcgcacg
    52741 ggccccacac cgcatttcgc tatatcgcgc cagcctcggt caaccgttca gccatgcggg
    52801 cgaactgcgc caccgacaat tcctcggcac gggcggtggc cggaatgccc aggccgtccc
    52861 aatccatcaa gcccgcccaa tcccccagcg cgcgccgcag catcttgcgc cgctgcgaaa
    52921 acgcgcgcgc caccacagcc gcgaatgcca cctcgtgttg cggggcgaca accgatgccg
    52981 gcaacggcac catgcgcacc acggccgaca cgacccgtgg cggcggatcg aacgactcgg
    53041 gcgccacgtc gaacagcttc tccatggcat aacgcgcctg gagcatgacc gacagccggc
    53101 cgtaatccga cgtggcgggc tg
//
LOCUS       DASTNK010000078        32852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_796464, whole genome shotgun sequence.
ACCESSION   DASTNK010000078 DASTNK010000000
VERSION     DASTNK010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 32852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32852
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796464"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1715
                     /gene="groL"
                     /locus_tag="VFR20_06075"
     CDS             75..1715
                     /gene="groL"
                     /locus_tag="VFR20_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014111035.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU0230118.1"
                     /translation="MAAKQVYFGDDARARIVRGVNILANAVKTTMGPKGRNVVLDRSF
                     GAPTVTKDGVSVAKEIELKDKFENIGAQLVKEVASKTSDNAGDGTTTATVLAQAIVEE
                     GLKYVAAGISPMDLKRGIDKAVAVAVDELHKLSKPCTTSKAIAQVGSISANSDTSVGE
                     IIANAMDKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINNQDKQVAVLEDP
                     YVLIFDKKISNIRDLLPILEQVAKSSRPLLIVAEDVEGEALATLVVNNIRGILKTTAV
                     KAPGFGDRRKAMLEDIAILTGGMVISEETGGSLEKATLEQLGQAKRIEVAKENTTIID
                     GFGDSKSIEARVKQIRVQIEEATSDYDREKLQERVAKLAGGVAVIRVGAATETEMKEK
                     KARVEDALHATRAAVEEGVVPGGGVALIRAKAAVAKVKGDNLDQESGIKLILRAIESP
                     LRTIVANAGDEPSVVVNNVANGKGNYGFNAATGEYGDLVEQGVLDPTKVTRTALQNAA
                     SIASLLLTTEAAVAEIVEDKPAAPAMPDMGGMGGMGGF"
     gene            complement(1850..2776)
                     /locus_tag="VFR20_06080"
     CDS             complement(1850..2776)
                     /locus_tag="VFR20_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0230119.1"
                     /translation="MNNLPDLEAWAIFAKVAETGSFARTAEALTLSQPTVSKAVSRLE
                     KRMKTVLFHRTSRRVSLTGSGEAALERASRILEEGEALEAEIAEQSSSLRGPIRMTVP
                     MSFGLPRLAPALPEFMSRYPDVTLDINFNDAQVDLIANRFDLALRISALVDSSLLARR
                     LCTVRLLLVGAPSYFERHGRPRHPRDLADHRALHYTYSHGGSNWHFRHARRGEFTQAM
                     PTPLRVNNAEALIPALRTGLGLALQPEFLAWQDLESGALESVMEEWRVDPIVLHIVTP
                     PGRNRPARVQALVRYLTERFSNEPWAQAIVPA"
     gene            2928..3629
                     /locus_tag="VFR20_06085"
     CDS             2928..3629
                     /locus_tag="VFR20_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEU0230120.1"
                     /translation="MIERRPLASLGGANHGWLDTKHHFSFAEYHDPSRMQWGALRVWN
                     DDTIAPGTGFPMHPHTNMEIITYVREGAITHRDSLGNGGRTVAGDVQVMSAGTGIVHS
                     EYNQEPGVTRIFQIWIMPDKLGGQPSWGSNPFPKDNRAGRFALLADGTTDGGDAMPIR
                     ANARVLGATLKAGEHVEYHFDAGRCGYLVPASGQVDVNGVSLQARDGAAIRDEGVIRV
                     TAQADAELVLVDAAA"
     gene            3740..4420
                     /locus_tag="VFR20_06090"
     CDS             3740..4420
                     /locus_tag="VFR20_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEU0230121.1"
                     /translation="MSKYLEVLTPQNSQIIFIDQQPQMAFGVQSIDRQTLKNNVVALA
                     KAAKAFHVPATITTVETDGFSGNTYPELLSVFPENKILERSSMNSWDDQNVRDALAAN
                     GRKKVVVAGLWTEVCNTTFALSAMLEGDYEIYMVSDASGGTSVDAHKYAMDRMVQAGA
                     VPMTWQQVMLEWQRDWARKETYDAVMGIVKEHSGAYGMGVDYAYTMVHKAPERVKHGR
                     RIGPNPAK"
     gene            4446..4727
                     /locus_tag="VFR20_06095"
     CDS             4446..4727
                     /locus_tag="VFR20_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XapX domain-containing protein"
                     /protein_id="HEU0230122.1"
                     /translation="MRMYAISLGAGLLVGVIYSVLNVRSPAPPLVALIGLLGMLIGEQ
                     VIPVGKQLMCGTAFVAACDKTHATNHVLGQLPGRQAAVAGAAASKEHQP"
     gene            4724..6625
                     /locus_tag="VFR20_06100"
     CDS             4724..6625
                     /locus_tag="VFR20_06100"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU0230123.1"
                     /translation="MNTETNEKASAEVILRNGRFTTLDKRRPLADAVAIRGGKFMAVG
                     EDGEVMKLASPTTRVIDLRGRAVLPGLIDNHTHVVRGGLNFNMELRWDGVRSLADAMD
                     MLKRQVAVTPAPQWVRVIGGFSEQQFVEKRLPTLDEINTIAPDTPVFLLHLYDRALLN
                     AAALRAVGYTRASQDPPGGQIVRDGDGNPTGLLLAKPNAGILYATLAKGPKLPFEYQV
                     NSTRHFMRELNRLGVTGVIDAGGGFQNYPEDYAVIQKLADEGRMTVRLAYNLFTQKPK
                     QEKEDFLNWTASTKYKQGDDYFRHNGAGEMLVFSAADFEDFRQPRPDMSQEMEGELEG
                     VVRILAENRWPWRLHATYDETISRALDVYEKVNKDIPLQGLNWFFDHAETISERSIDR
                     IAALGGGVAVQHRMAYQGEYFVERYGAAAAEATPPIARMLEKGLKVSAGTDATRVASY
                     NPWVSLAWMITGKTVGGMRIYPQRNCLDRETALRMWTENVAWFSDEEGRKGRIAQGCF
                     ADLIVPDKDFFSCPADDISYLTSELTLVGGRVVYAAGAFATLDDNAPPPAMPDWSPVR
                     RFKGYAAWGEPQGAGRNSLSYRHMGACGCANACTVHGHRHLAAWLPDAPVSDTQGFFG
                     ALGCSCWAV"
     gene            6630..7088
                     /locus_tag="VFR20_06105"
     CDS             6630..7088
                     /locus_tag="VFR20_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU0230124.1"
                     /translation="MASWEKPSAASPGGRPLVPGGGEDSQHASGVVCLIAYLGLCAAY
                     IQGGLDKLMDFHGAIGEMTYFGLSPAPLFAALVILLELLGSAMVLTGFFRWLGALALG
                     AFTLMATFLALRFWELPLGQERFMAANSFFEHLGLVGGFLLVAWLDLRRK"
     gene            7090..7776
                     /locus_tag="VFR20_06110"
     CDS             7090..7776
                     /locus_tag="VFR20_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010107998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HEU0230125.1"
                     /translation="MKLNLPTILSFNGGYVDTAGFLALQGLFTAHVTGNFVTLGAAIA
                     HGSSGIIAKLLALPIFCVTIVIVLYAMRHISHSPRRSLLTMLVLKFVFLTAGAACAIH
                     YGSFIDSDGWPLILTGALLIIAMAIQNAAHRIHLGSAPPSTLMTGTTTQIMIDLANLL
                     RGASNEAPEVVKARLRRLTIAVVAFALGCGFAALFYIEFQMWCFIVPPLSALTGIAIL
                     MAENQGPQTD"
     gene            8082..8474
                     /locus_tag="VFR20_06115"
     CDS             8082..8474
                     /locus_tag="VFR20_06115"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU0230126.1"
                     /translation="MTPQHSSYKSKGGPGRLLNALRYSVQGLGAALRHEAAFRQELLV
                     AVVLTPVAFWAGRTPTQVLLLMGAMVFVLIVEVLNSALEALADSITTDPHPMIGRAKD
                     LGSAAVMLSIALAVAVWLVILLTNANAP"
     gene            8542..9369
                     /gene="pyrF"
                     /locus_tag="VFR20_06120"
     CDS             8542..9369
                     /gene="pyrF"
                     /locus_tag="VFR20_06120"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU0230127.1"
                     /translation="MTFIEQLNQSWTNSNSLLMVGLDPDPARFPTELRGRDDAVFEFC
                     KAIVDATADYVCGIKPQIAYFSAHRAEDQLEALCRYVHDRHPRLPIVLDAKRGDIGST
                     AEQYAREAFERYQADAVTVNPFMGFDSLEPYLARPDKGVIVLCRTSNPGGSDLQFIES
                     ADGTPLYLRVAELVAGRWNTNGQCALVVGATFPEEVAKVRQRIGDMPLLIPGIGAQGG
                     DVTATVAAGRDSQGAGMMINSSRAILYASQNDDWREAASRVARDTRDAINAARAGLP"
     gene            complement(9430..9921)
                     /locus_tag="VFR20_06125"
     CDS             complement(9430..9921)
                     /locus_tag="VFR20_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEU0230128.1"
                     /translation="MIASTDLARELGQKLLGRGWMLVCAESCTGGLLSAAVTDIPGSS
                     QWFERGFITYSNAAKMAHLRVSADTLERFGAVSEETAMEMAAGALGAVATANLSIAVT
                     GIAGPDGATPGKPVGMVCFGFSRQAGEGVVTRAATHIFEGDRRQVREAAVRFALDAAA
                     KFI"
     gene            complement(9927..10451)
                     /locus_tag="VFR20_06130"
     CDS             complement(9927..10451)
                     /locus_tag="VFR20_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEU0230129.1"
                     /translation="MPNDDSRNPQASRPDPNAAWVFKTPWRIAAFGLGSGLLRPGPGT
                     WGTLLGWLLWFLILGRVPDAAAGVVLLVAFIAGCWCCHRTGQELGITDYGGMVLDEIV
                     AFWLVLWLVPASFGAQLLAFIVFRVFDILKPPPIHFFDAHIKNGFGVMWDDIVAAAYT
                     LLVMAVLIRLQGML"
     gene            complement(10478..11440)
                     /gene="thiL"
                     /locus_tag="VFR20_06135"
     CDS             complement(10478..11440)
                     /gene="thiL"
                     /locus_tag="VFR20_06135"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEU0230130.1"
                     /translation="MSSEFDLIARFFSRPAPPGFLGVGDDCALLPLEPGMRLATSTDL
                     LLEGRHFLPGTDPGNLGHKALAVNLSDLAAMGAKPLACVLGLSVPKVDEPWLRAFSEG
                     FHSLADEAGCPLVGGDTTRSPWGVVISVTVYGQVDPARALRRDAARVGDDIWISGTLG
                     AADIALRLLQGKLPLDPGRLAALRPALERPQPPLRLAQALAGVAHAALDISDGLAQDL
                     GHILEASHCGAELDLAALPIDPGLDGLDSGLRRQAALDGGDVYQLCFTAPSDKRDIIA
                     RLALAASTRVTRVGRIVEHAGLAVQSADGGLAPYHSAGFDHFSS"
     gene            complement(11460..11951)
                     /gene="nusB"
                     /locus_tag="VFR20_06140"
     CDS             complement(11460..11951)
                     /gene="nusB"
                     /locus_tag="VFR20_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU0230131.1"
                     /translation="MTANKPSRGPAASRANTRSARRRAREFALQGIYSWLLRGGGMAE
                     TGEIDAHIRDDEEFSEADAEWYKTLLHGVFREAEGLRERFMPYIDRPLAELSPIEHSI
                     LLIGCYELVFHVEVPYRVAINEAVELAKSFGGTDGFKFVNGVLDKVAADVRSAEVGAA
                     SRR"
     gene            complement(11938..12486)
                     /gene="ribH"
                     /locus_tag="VFR20_06145"
     CDS             complement(11938..12486)
                     /gene="ribH"
                     /locus_tag="VFR20_06145"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU0230132.1"
                     /translation="MNPYTLSPDLNGEGLHIGVVRARFNEDIGLAELDACLAELARLG
                     VDERDVMVVSVPGALELGVTLAQMAETFEFDALIALGAVIRGETYHFELVSNESASAI
                     SRVSLETGIPVANGVLTTDTDEQAEVRAADKGRDCAAAAVEMANLVAALEPESEEDDD
                     DDDELDDDEDDDQAEEGHLDRK"
     gene            complement(12501..13655)
                     /gene="ribBA"
                     /locus_tag="VFR20_06150"
     CDS             complement(12501..13655)
                     /gene="ribBA"
                     /locus_tag="VFR20_06150"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU0230133.1"
                     /translation="MSDAASSSGILPESFDISPVSEIVAELRAGRVVILVDEEDRENE
                     GDLVLAADYVTPDAINFMVTHARGLVCLTLTEERCRQLGLSLMASSNGSRFGTNFTLS
                     IEAAEGVGTGISAADRARTIQAAVARDARPADIVQPGHIFPVRAVRGGVLVRAGHTEA
                     GCDLTRLAGLTPAAVICEVLNPDGTMARLPDLKLFAQRHGLKIGTIAELIQYRSEHES
                     MVERVSSRMVRTPWGEFQAVAYRDLASGAPHLALVHGDISPDTETLVRVHEPTSMLDV
                     LCDDSSGHSWGVSSALKAIAAVPSGVVVLMNCQGSADLVFGQFESWAAPERSVEAPKF
                     DLKTYGIGAQILRDLNVGKARLLARPRKMPSMAGFSLTITGYDSEPTPQP"
     gene            13775..14584
                     /locus_tag="VFR20_06155"
     CDS             13775..14584
                     /locus_tag="VFR20_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU0230134.1"
                     /translation="MPEPRTLENDGRRGIQSVETGFPLLAALVEAGTALTLRDLSARA
                     GMASAKAHPYLVSFVRVGLVQQDATTGRYELGPFALQMGLVSLQRLDPVRVALPCVNR
                     LAGEIGHTLAIAVLGSHGPTMIHICEASYPVHVNMRKGTVMSMLHTATGRVFAAWLPP
                     RIAQHYIEREEGDQAVVTSVGQPPVDPTAFEAQLAEIRRRGIERTLGNPLPGIDAFSV
                     PVFDHSGGVTLALTSLGPSSLFDSRWDGSIARSLTACARDISTSLGYRAPA"
     gene            complement(14653..15876)
                     /gene="ftsY"
                     /locus_tag="VFR20_06160"
     CDS             complement(14653..15876)
                     /gene="ftsY"
                     /locus_tag="VFR20_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013207055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU0230135.1"
                     /translation="MFSFLKKKNVPRPGGEDQAADPAAPGAPTGDDAAAGTRAAESDS
                     SGSEHARAPSEDTAAPELSGAHAAADGSTGSVSDAHDMALSQPVVPPETSAAVAAPAA
                     GKSWLSRLRSGLSRTGQSIGGLFVGVKVDEALFEELETALIMADAGVAATEQLLTALR
                     ARVRKERLSDASQVRLALRDILAEHLAPLEKSFPLGSVAPLIVMIAGVNGAGKTTSIG
                     KLANAFQRQGASVLLAAGDTFRAAAREQLMAWGARNNVTVIAQEGGDPAAVAFDAVNA
                     GRAREAGVVMIDTAGRLPTQLHLMEELKKIRRVIGKADAAAPHEVLLVIDGNTGQNAI
                     SQIKAFDAAINLTGLVVTKLDGTAKGGALAAVAAGSQGVRPVPVYWIGVGEGLDDLQP
                     FVAREFATALVGEEP"
     gene            15961..16617
                     /locus_tag="VFR20_06165"
     CDS             15961..16617
                     /locus_tag="VFR20_06165"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEU0230136.1"
                     /translation="MKKHLLRIVGGDYRRTPIPVVDAEGLRPTPDRVRETLYNWLHHF
                     WGGGFSDKRVLDLFAGTGALGFEAASRGAAHVQMVEANPEAVAALRQLRTRLGASQVR
                     IHAGDARTVLARLNASPFDLVLLDPPFGLDWLDKLWPALPALMAPDALLYVESETPVA
                     PPLAFEIVRQGKAGAVHFHLLRFAASQKTGNNSTSESTQPGDQSGHHASEPSQQPEPE
                     "
     gene            16614..17117
                     /gene="coaD"
                     /locus_tag="VFR20_06170"
     CDS             16614..17117
                     /gene="coaD"
                     /locus_tag="VFR20_06170"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU0230137.1"
                     /translation="MIIAVYPGTFDPLTRGHEDLVRRAASLFDHLVVGVAHSRNKKPF
                     FSVEERVEIAREVLGHYPNVEVKSFAGLLKDFVREQNGRVIVRGLRAVSDFEYEFQMA
                     GMNRHLLPEVETLFMTPSDQYQFISGTIVREIAILGGDVGKFVFPSVERWLHSKAKAL
                     REAGEQE"
     gene            complement(17205..17597)
                     /locus_tag="VFR20_06175"
     CDS             complement(17205..17597)
                     /locus_tag="VFR20_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEU0230138.1"
                     /translation="MKPNDMYFPIAACAGCDHPDAADYDKRWLVVDENGRLVTRDEFP
                     LLGEIAPAIRFGYLAIKAPGMLRMDIPLDVIEDDDSVRRVATIGTQKVDVVDEGDLAA
                     TWLTNYLKAPCRLMKVHPDAGKVDWPVL"
     gene            complement(17594..20245)
                     /locus_tag="VFR20_06180"
     CDS             complement(17594..20245)
                     /locus_tag="VFR20_06180"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEU0230139.1"
                     /translation="MKVWFKRILIAIVVLVIATLVGIAIFLLTFDPNAYKAKLERLVY
                     SRYHRTLAIKGPLELSLFPRIGLSVHDVSLSDVNSDDTFASIDSARFAVAIWPLISNR
                     LVVDHVAVSGFKAWIARDKHRRFNFDDLLGSRPPYAASQSAPKMGAVAHATPIALAGG
                     AAQQLVSLPAAIADRHAARTDFQIDIAGLDLKGGEIHYFDAVTGTSARVQHLDVNTGR
                     VTFGQPFDVALKGKLIGDYPVAKADIQGQAVVTFNPEARTYSAQKLNLQLKGLLGSLD
                     AKSATLQGNLAYDAYSRMLDVSNIELSVQGNVHPSKLGVPQIKNLDVRLAVPRLRVDR
                     SRWEFQVQKLALRAKGNLPDRAFDVAFDAPSLSISPDSAKGEPVAGTVKLTSDNEVLG
                     VALGLAGIDGNAYNLSFKELKVDSGLKQGDRLVQLNLSSPGSWNFLSEQGGLSAIKGD
                     MKIQDAALPGGSFDFPMIGSLSADLAKDQIVSEIDAVLNGSKLDFKLKATHLNDPAVA
                     FDLNADSLDFNKLFPPVTGKPDAKPTAAPGKPAAKKAAPSTSGKAGPATQAAPAPSEP
                     IDLKFLDSANVTGTIKIGDLKVGQFEAKKFSAAVRAAKGTLDVTRVTADLYQGTLTGG
                     FTATSNGDLATKASLRNVSVLPMLLALGQPGRLSGLGSLNVDLRTTGTTADACVAGLT
                     GTVKAQVHDGAITGINVAQTLREVSQAVKNVLNGQTSSLATTFDAARKTDFTSLDASL
                     VFSQGQGTLKNLSIMAPMLRITEGEPSSIDLVNKQLDIMVKARVVGSAKGQGGLEELE
                     GVTVPVRIAGPFSKPGYQVQWKEIGSHAVKQAVQRGLLDLLSKGVGGGPAPAASPASP
                     ESKPAPRPEPSSTVKSIGNALKGLLGK"
     gene            complement(20519..21022)
                     /locus_tag="VFR20_06185"
     CDS             complement(20519..21022)
                     /locus_tag="VFR20_06185"
                     /inference="COORDINATES: protein motif:HMM:NF039485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HEU0230140.1"
                     /translation="MAGHGKIRQFFLPVAIWAVHSNSNNQGASIMGLLDSVMSAVGGN
                     ANSGQAASLLPALVAEINNYPGGLQGLVQKFQAGGLGDVVSSWVGTGANQSVSSDQVQ
                     SVLGDDVVNNLAQSTNQDKSSVLAGLSSLLPHVVDQATPNGSVPDGQSLDASSLMGAV
                     SGLLGKL"
     gene            21122..22240
                     /locus_tag="VFR20_06190"
     CDS             21122..22240
                     /locus_tag="VFR20_06190"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEU0230141.1"
                     /translation="MLIGIPRESLIGETRVAATPETVKKYVKAGHTLVIEQGAGEAAR
                     YPDQAFTDAGAELGDGSRALSCDLVLKVRSPDSSELGRMKPGATLVGMLDPFNREGLE
                     AFAAAGISAFALEAAPRITRAQSLDVLSSQANLAGYKAVLLAANHYQRLLPMMMTAAG
                     TLKAGRVVILGAGVAGLQAIATARRLGAMVEASDVRPAAKEQIESLGAKFIDVPFETD
                     EERDIAQGVGGYARPMPAAWMARQAVLVAERCRQADIVISTALIPGRPAPVLITEDTV
                     KSMKPGSVIVDLAVERGGNCPLSEKDKVVEKHGVILVGHSNLPALVPTDASALYARNV
                     QDFLKLIISPDGGLAIPQDDEIVTACLVCAGGKVLRSA"
     gene            22240..22551
                     /locus_tag="VFR20_06195"
     CDS             22240..22551
                     /locus_tag="VFR20_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEU0230142.1"
                     /translation="MEAVNPTLINFIIFVLAVYVGYHVVWNVTPALHTPLMAVTNAIS
                     AIVIVGAMLAAGLTDGYLGRFMGVFAVALAAVNVFGGFLVTRRMLEMFKKKDRKTGGR
                     A"
     gene            22548..23984
                     /locus_tag="VFR20_06200"
     CDS             22548..23984
                     /locus_tag="VFR20_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEU0230143.1"
                     /translation="MISTNLVTLLYLIASVCFIQALKGLSSPTTSRRGNAFGMVGMAI
                     AVLTTAALIVGLATQGTAVIGLGWIILGLLIGGTFGTFLAKRVEMTKMPELIAFMHSM
                     IGLAAVAIAIAVISEPHAFNIALPGQPLPTGNRFELVIGTFIGAITFSGSVIAFGKLS
                     GTYKFRLFKGAPVVFQGQHLLNLVLVIVMLAFGAGFIATQSWLPYVIMLLLAFALGVL
                     IIIPIGGADMPVVISMLNSYSGWAAAGIGFSLNNPMLIIAGSLVGSSGAILSYIMCKA
                     MNRSFFNVILGGFGNEGGAAQTGEHAQRNVKSGSPEDAAFLMSNAETVTIVPGYGLAV
                     ARAQHALKELTEKLSIAGVTIKYAIHPVAGRMPGHMNVLLAEADVPYDQVFEMEDING
                     EFPQTDVVLVLGANDVVNPAAKNDPKSPIAGMPILEAYKARTVIVNKRTMAAGYAGLD
                     NELFYMDKTMMVFGDAKKVIEDMIKALE"
     gene            complement(24011..25507)
                     /locus_tag="VFR20_06205"
     CDS             complement(24011..25507)
                     /locus_tag="VFR20_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0230144.1"
                     /translation="MTTSELLEGLGVAPSRLQGGTLAVHSPIDGAEIARLREHARDDT
                     IAAIGEAKNAFLAWRTVPAPRRGELIRLFGHELRASKAALGRLVSIEAGKILAEGMGE
                     VQEMIDICDFALGLSRQLYGLTIASERPGHRMMETWHPLGVVGVISAFNFPVAVWSWN
                     TALAIVCGNAVVWKPSEKTPLTALACQALFERALAKFGDAPQGLSQVLIGGRDVGEAL
                     VDDERVALLSATGSTRMGRQVGPRVAARFGRSLLELGGNNAIIVGASADLGLAVRGIL
                     FGAIGTAGQRCTTTRRLIVHESVADDLVARLKTAYASARVGNPLEPDTLVGPLIDVAA
                     FEAMQKALALAREQGGNVFGGERMLAAEFPAARYVSPALVEMPGQTDVVCQETFAPIL
                     YVMRYRDFDEALRLQNAVPQGLSSAVFTMDLREAETFLSVSGSDCGIANVNIGTSGAE
                     IGGAFGGEKETGGGRESGSDAWKTYMRRATNTVNYSRELPLAQGIQFG"
     gene            25761..26942
                     /locus_tag="VFR20_06210"
     CDS             25761..26942
                     /locus_tag="VFR20_06210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU0230145.1"
                     /translation="MPFDTYDVIIAGGAAAGSAAAYFLTANPDFNGKVLVVEKDFTYQ
                     HCATTLSAASIRHQFSTPENIRMSQFGTQFLRGFGATMAVDGIEPDAAFHEAGYLFLA
                     TSAGMNVLRENHATQKDLGVDVALLNPTQLTQRYPWLNTSDLAAGSLGLSGEGWLDAY
                     GMMQGLRRKAIAQGARYIENTVTGVRHEGRRIVGVTLSDGTHLDCGTLINTAGTGAPA
                     LARLAGIALPVEARKRCVFHFQCPAPLPDCPMVIDPSGAYFRPEGNGFIGGISPDEAH
                     DPECFDFDVQHEVFEDVLWPLLAQRVPAFEALRRLNAWAGHYDMNTVDHNVIIGAHPD
                     IDNMLFANGFSGHGMQQSPAVGRALSELVTWGGFRTLDLSRLGWARILEHRPIVEKNI
                     V"
     gene            27031..27657
                     /locus_tag="VFR20_06215"
     CDS             27031..27657
                     /locus_tag="VFR20_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU0230146.1"
                     /translation="MNRTPSTSNPPALPERLRSLRQSRGWTLDRTGKASGVAASTLSK
                     IENGLMSPTYDVLLKLADGLGLDVAELFAPARAHMGAGRCSVERQDGGEIHETPLYRH
                     RLLCSQLSHKRMMPFVTTIKAHSLAENEGWSRHDGEEFVYVLKGAVELHTEYYQPTTL
                     KAGDCFYIDSRMRHRVVNRGKIDAKVIWVSTQQGVPGQHALNHEGHAD"
     gene            27683..28603
                     /gene="lhpI"
                     /locus_tag="VFR20_06220"
     CDS             27683..28603
                     /gene="lhpI"
                     /locus_tag="VFR20_06220"
                     /EC_number="1.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047125 - delta1-piperideine-2-carboxylate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0050241 - pyrroline-2-carboxylate
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     Delta(1)-pyrroline-2-carboxylate/Delta(1)-piperideine-2-
                     carboxylate reductase"
                     /protein_id="HEU0230147.1"
                     /translation="MTTFQTLSVFNAEETANLLPFPELVTAIATASAQCARGDIDAPE
                     RQVVPLARGAGVMLSMPATAEDVAIHKLVNVVPGNRARGLPTIHGVVTAYDGATGREL
                     FVLDGPTVTARRTASVSMLGLKTFCAKPPRRIALIGTGKQASGHVQAIAALYPGIDLC
                     IVGRSQAKAREFVAAHDGYPLTFHAATEAPPDIDAALTTTTSKTPVYHLPARAGRLVI
                     GVGAFEATAAEISPETVQASQLYVDDPAGAHQEAGDLILAGVDWAAVRALADALAHGA
                     DTARPIVFKTVGCAAWDLAAARCAMARLQP"
     gene            28714..29481
                     /locus_tag="VFR20_06225"
     CDS             28714..29481
                     /locus_tag="VFR20_06225"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar regulator YcgR PilZN domain-containing
                     protein"
                     /protein_id="HEU0230148.1"
                     /translation="MYIATPGLLHDHDDPMLITGRLAITSLLRSLKERKALVGMHLRG
                     STLTVITAILQVDETRGLFIIDAARDAQFNKRATDAEDIRFESALDGIGLKFSGAYAS
                     PCHHEGKPALCLALPKSLQRIQRRESYRVEVPVSQPARCTLTPTDQNGSVATAITLDL
                     KDISLGGVSLIDNLKQLQADQGSLYPNCQIVLPEAGTITATLRVVRILKSTLENGKTV
                     RHLGCAFVGLPNPMSTIVQRYVGLLERKLSAKRYGID"
     gene            complement(29488..30345)
                     /locus_tag="VFR20_06230"
     CDS             complement(29488..30345)
                     /locus_tag="VFR20_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0230149.1"
                     /translation="MTTASAWVSGFIGMLIFSGSLPATRVAVQGLDPTFVTVARASIA
                     GLLAIALLVVRREGIPVRRDLMSLVVVALGVVLGFPFLSALALQHITAAQSVVFVGVL
                     PLSTSLFGVIRGGERPRWGFWLFAVLGAAAVVGYAFSHQAPSSRTGAWLMLGAILVCG
                     LGYAEGAVLSRRLGGLAVICWALVVALPVMLVALWMAWPATFANVPPQAWVGLGYVSL
                     FSMLIGFVFWYRGLAGGGIATVGQLQLLQPFFGLLLAATLLGEPYTLAMIVVMVVAAL
                     CVAGARRMA"
     gene            30530..31957
                     /locus_tag="VFR20_06235"
     CDS             30530..31957
                     /locus_tag="VFR20_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU0230150.1"
                     /translation="MNTLVTGATRIEQTMDAIRRRIALRQLTPGARVPSIRQFAADTG
                     VSKSTVVEAYERLAAEGVIHPRRGSGFYVAGHLAPLALSELGPRLDREVDPLWVARQS
                     LDSGTLALKPGCGWLPNEWMPQDDIRRALRGIARAPHTPLTDYAPTLGQPGLRRLLAQ
                     RLQERGMNTTPAQILLTDSGTQAIDLLCRFLIEPGDTVLVDDPCYFNFLALLRAHQVR
                     IVSVPFTTTGPDLELFAATLAEHQPRLYITNSALHNPTGASLSPLAAHRILKLAEVHD
                     LVIIEDDIFSDFESSPSTRLAAFDGLERVIQIGSFSKTLSASARCGYIAARADWVDSL
                     ADLRIATAFSGCALTQSLVQALLEGGGYRRHVETLRQRLADATAPVMTRLERLGITPW
                     AAPRGGMFLWCRLPHGLDGARLAGLALAQGLVLAPGNVFSLSQTASGFMRFNPSQMAG
                     PRVFELLETAMNQAATMADEPRAAA"
     gene            32030..32821
                     /locus_tag="VFR20_06240"
     CDS             32030..32821
                     /locus_tag="VFR20_06240"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011325926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU0230151.1"
                     /translation="MENPGIDDQQTSPSAPVVQNAFQGDLMAHVQALPDTWRQAFDDE
                     QTQGTLHDLASFLDDELARGMEIFPLRPFRALLETDPDAVQVVILGQDPYHGPNQAQG
                     MSFSVPDPCPTPPSLRNMFAELAREYEGRFERQRNSLLRWARQGVLLLNTSLSVEAHK
                     PASHAKRGWETITDAIVARVLREPRPKVFLLWGSHAQSKEALLREHPPQGPVLVLKAN
                     HPSPLSALRPPRPFIGCGHFQKANEWLQGHGETGIDWLQNTTSPR"
BASE COUNT         6234 a        11188 c         9915 g         5515 t
ORIGIN      
        1 tcatccgcga agaagaagtc ttcgccgtga tccagtaacc cactgctccg ttcaaatacc
       61 caaggaattt caacatggct gcaaagcaag tctattttgg tgacgacgcc cgcgcccgca
      121 tcgtgcgcgg cgtcaacatc ctggccaacg ccgtcaagac caccatgggc cccaagggtc
      181 gcaacgtggt gctggaccgc tccttcggcg ctcccacagt gaccaaggac ggcgtgtccg
      241 ttgccaagga aatcgaactg aaggacaagt tcgagaacat cggcgcccag ctggtcaagg
      301 aagtggcctc caagacgtcg gacaacgccg gtgacggcac cacgacggca acggtgctgg
      361 cccaggccat cgtcgaagaa ggcctgaaat acgtggccgc cggcatcagc cccatggacc
      421 tcaagcgcgg catcgacaag gccgtcgccg ttgccgtgga cgagctgcac aagctctcca
      481 agccctgcac caccagcaag gccatcgccc aggtcggttc gatctcggcc aacagcgaca
      541 cctccgtcgg cgaaatcatc gccaacgcga tggacaaggt cggcaaggaa ggcgtcatca
      601 ccgtcgaaga cggcaagtcg ctggaaaacg aactggacgt cgtcgaaggc atgcaattcg
      661 accgcggcta cctgtcgccc tacttcatca acaaccagga caagcaagtc gccgtcctgg
      721 aagatccgta tgtcctgatc ttcgacaaga aaatcagcaa catccgtgac ctgctgccca
      781 ttctggaaca agtcgccaag tccagccgtc cgctgctgat cgtggctgaa gacgtcgaag
      841 gcgaagcgct ggccactctg gtcgtcaaca acatccgcgg catcctgaag accacggctg
      901 tcaaggcgcc tggcttcggc gaccgtcgca aagccatgct ggaagacatc gccatcctga
      961 cgggcggcat ggtcatctcc gaagaaaccg gcggctcgct cgaaaaagcc acgctggaac
     1021 agctcggcca ggccaagcgc atcgaagtgg ccaaggaaaa cacgaccatc atcgacggtt
     1081 tcggtgacag caagtcgatc gaagcgcgcg tcaagcagat ccgcgtccaa atcgaagaag
     1141 ccacgtccga ctacgatcgc gaaaaactgc aggaacgcgt ggccaagctg gctggcggtg
     1201 ttgccgtgat ccgcgtgggt gccgcgacgg aaaccgaaat gaaggaaaag aaggcccgcg
     1261 ttgaagacgc gctgcacgcc acgcgtgccg ccgtcgaaga aggcgtggtc cccggcggcg
     1321 gtgtcgcgct gatccgtgcc aaggccgccg tggccaaggt caagggtgac aacctggatc
     1381 aggaatcggg catcaagctg atcctccgcg ccatcgaatc gccgctgcgc accatcgttg
     1441 ccaacgctgg cgacgaaccc agcgtggtcg tcaacaacgt ggccaacggc aagggcaact
     1501 acggcttcaa cgccgccacc ggcgagtacg gtgacctggt cgaacaaggc gtgctggacc
     1561 ccaccaaggt cactcgcacc gccctgcaga acgcggcgtc gatcgccagc ctgttgctca
     1621 cgaccgaagc cgctgtggcc gaaatcgtcg aagacaagcc ggccgctccc gccatgcccg
     1681 acatgggcgg catgggtggc atgggcgggt tctaaggaac ccttcccggc cggccggcgg
     1741 cttgccgccg gccacaatgc cgtggtgcgt gcaggcttcg gccgacgcgc gccgcagtcc
     1801 ggaaagcaga accgccgcca ttgtgcggcg gttctgcttt ttttggccgt tacgccggca
     1861 cgatggcctg tgcccagggc tcgttggaaa aacgctcggt caggtagcgc accagcgcct
     1921 ggacacgagc gggacgattg cggcctggcg gcgtgacgat gtgcaggacg atcgggtcca
     1981 cgcgccattc ttccatcacg gattccaaag cgcccgattc caggtcctgc caggccagga
     2041 attcgggctg caacgccaac cccaggccgg tgcgcaaggc gggtatcagg gcttcggcgt
     2101 tgttgacacg cagcggcgta ggcatggcct gggtgaactc gccgcggcgg gcatgccgga
     2161 agtgccagtt cgaaccaccg tgcgagtagg tgtaatgcag ggcgcgatga tccgccagat
     2221 cccgggggtg tcgagggcgc ccgtggcgct cgaagtagga cggcgcaccg accagcaaaa
     2281 gacgcaccgt gcacagtcgc cgcgccaaca ggctcgaatc gacaagcgcg gaaatgcgca
     2341 gcgccagatc gaaacggttg gcaatcagat caacctgggc gtcgttgaag ttgatgtcga
     2401 gcgtgacgtc gggatagcgc gacatgaatt ccggaagcgc cggcgccagc ctcggcaggc
     2461 caaaagacat tggcaccgtc atccggatgg gcccgcgcag gctgctggac tgttccgcga
     2521 tttccgcctc caacgcttcc ccttcctcga ggatgcgtga cgcgcgctcc agcgcggcct
     2581 cgccgctgcc cgtgagtgaa acgcgccgcg acgtgcggtg gaacagcacg gtcttcatcc
     2641 gcttttccag gcgcgagacg gccttggaca cggtgggctg ggatagcgtc agcgcctcgg
     2701 cggtgcgcgc gaaagagccg gtttccgcca ccttggcgaa aatggcccag gcttccagat
     2761 cgggcagatt attcatcgtt actcaggcag gccgcatggc gtggaatggg cctgcccctc
     2821 catttattaa taaaattttg gaaagcatgt tattcgatta attccattct catagtattc
     2881 aacggcgatt atagtgggtc ccatgctcaa gcaaacagga gatttccatg atcgaacgtc
     2941 gtccactggc aagcctcgga ggcgccaacc acggttggct cgacaccaag caccactttt
     3001 ctttcgctga ataccacgac ccgtcgcgca tgcagtgggg cgccctgcgg gtatggaacg
     3061 atgacacgat cgcccccggg acgggatttc ccatgcaccc gcacacgaac atggaaatca
     3121 tcacctacgt gcgcgagggc gccatcaccc accgggacag cctgggtaat ggagggcgga
     3181 ctgtggcggg cgacgtccag gtcatgagcg ccgggacagg catcgtgcac tccgaataca
     3241 accaggagcc gggcgtcacg cgcattttcc agatctggat catgcccgat aagctcggcg
     3301 ggcaaccgtc gtggggctcg aaccctttcc cgaaggataa ccgtgcagga aggttcgcct
     3361 tgctggccga cggaacgacg gacggcggag acgctatgcc gattcgcgcc aatgcgcgcg
     3421 tgctgggcgc cacgctcaag gctggcgaac acgtcgaata ccacttcgac gccggccgct
     3481 gcggctatct ggtgccggct tccggacagg tggacgtcaa cggcgtctct ttgcaggctc
     3541 gcgatggcgc ggcaatcaga gacgaaggcg tgatacgggt gaccgcccag gcagacgccg
     3601 agttggtgct cgtcgacgcc gccgcctagc agtttgcctg gctgactcat gcatccgaat
     3661 ccgggtgcat gggcgtgccg ggccggaaca ctttgctgca ctcatcctcg cattgccccc
     3721 atcgaaggag aaaaccatca tgtcgaagta tctagaagtt ctcacgcctc aaaacagcca
     3781 gatcattttc atcgaccagc agccacaaat ggcgttcggc gtgcagtcca tcgaccggca
     3841 gacgctcaag aacaacgtcg tcgcactggc caaggcggcc aaggcattcc atgttcccgc
     3901 caccatcacg acagtggaaa cggacgggtt ttcgggcaac acctacccgg aactgctgag
     3961 cgtctttccg gagaacaaga tcctggagcg ctcgtccatg aactcctggg acgaccagaa
     4021 tgtccgggat gcgctggccg ccaacggccg caagaaggtg gtcgtcgcgg gcctgtggac
     4081 ggaggtgtgc aacaccacgt tcgcactgtc ggccatgctg gaaggtgatt atgaaatcta
     4141 catggtgtcc gacgcatcgg gcggcacctc cgtggacgcg cacaagtacg ccatggatcg
     4201 catggtccag gcgggcgccg tgcccatgac gtggcagcag gtcatgctgg agtggcagcg
     4261 cgactgggcg aggaaggaaa cctacgacgc ggtcatgggc atcgtcaagg aacactccgg
     4321 ggcgtatggc atgggcgtgg actacgccta cacgatggtc cacaaggcac cggaacgcgt
     4381 gaagcacggc agacgcatcg gccccaatcc cgccaagtaa gcgcaaccga gagccgaggt
     4441 gaaccatgag aatgtatgcc atatccctcg gcgccggcct gctggtcggc gtcatctata
     4501 gcgtgctgaa cgtccgctcg cccgcgccac cgctggtcgc cctgatcggc ctgctcggca
     4561 tgctgatcgg agaacaagtc attccggtcg gcaagcagct catgtgcggt accgcattcg
     4621 ttgccgcgtg cgataagact cacgccacca accacgtcct gggtcaactg cccgggcgcc
     4681 aagccgccgt ggcgggagcg gccgcctcaa aggagcatca gccatgaaca cggaaacaaa
     4741 cgagaaagcc tctgccgagg tcatcctgcg caacggccgc ttcacgacgc tcgacaagcg
     4801 ccggccgctg gccgatgccg tggccatccg aggcgggaaa ttcatggcgg tgggagaaga
     4861 cggcgaggtc atgaagctgg ccagcccaac cacccgagtc atcgacctgc gcggccgcgc
     4921 cgtgctgccc ggcctcattg acaaccacac acacgtggtg cgaggcggcc tgaacttcaa
     4981 catggaattg cgctgggacg gcgtacggtc gcttgccgat gccatggaca tgctcaagcg
     5041 ccaggtggcg gtgacgcccg cgccgcagtg ggtgcgtgtc atcggcgggt tcagcgagca
     5101 gcagttcgtt gaaaagcggc tgcccacgct cgacgagatc aacaccatcg cgcccgatac
     5161 gcccgtcttc ctgctgcatc tgtacgaccg cgcgctgctc aacgctgccg ccttgcgcgc
     5221 tgtgggctac acccgtgctt cgcaagaccc tcccggcggg caaattgtgc gcgacggaga
     5281 cgggaacccg acggggctgc tgctcgccaa gccgaacgcc ggcattctgt atgcgaccct
     5341 ggccaagggg cccaagctgc cgttcgaata ccaggtcaat tcgacccgcc atttcatgcg
     5401 ggaactgaac cgcctgggcg tgacgggcgt gattgatgcg ggcggcggct tccagaatta
     5461 ccccgaagac tatgcagtca tccagaagct ggccgacgaa ggtcggatga cggtccggct
     5521 ggcctacaac ctgttcacgc agaaacccaa acaggaaaag gaagacttcc tcaactggac
     5581 ggccagcacc aagtacaagc agggcgacga ctatttccgc cacaacggcg cgggcgagat
     5641 gctggtgttc tccgccgccg acttcgagga cttccgccag cctcggcccg acatgtccca
     5701 ggagatggaa ggggaactgg aaggcgtggt gcgtatcctg gccgagaacc gctggccctg
     5761 gcggctgcat gcgacctacg acgagacgat ctcgcgtgcg ctggacgtct atgagaaagt
     5821 gaacaaggac atcccgctgc aggggctgaa ctggttcttc gaccacgccg aaacgatctc
     5881 cgaacgctcc atcgaccgca ttgcggcatt gggcggcggc gtggcggtac agcatcgcat
     5941 ggcgtaccag ggcgaatact tcgtcgagcg ctacggcgcg gccgccgccg aagccacccc
     6001 tccgatcgcc cgcatgctgg agaaaggcct gaaggtatcg gcgggcacgg acgccacgcg
     6061 cgtggcctcc tacaatccct gggtgtcgct tgcctggatg atcaccggca agacggtggg
     6121 tgggatgcgg atatatccgc agcgcaactg tctggatcgc gagacggcgc tgcgcatgtg
     6181 gacggaaaac gtcgcatggt tctccgacga ggaaggtagg aaggggcgta tcgcgcaagg
     6241 ctgcttcgcc gacctcatcg tgccggataa ggatttcttc tcttgcccgg cagacgatat
     6301 ttcctacctc acgtccgagc ttacgctcgt gggcgggcgc gtcgtctacg cagccggcgc
     6361 tttcgcgacg ctggacgaca acgcgccgcc gcccgccatg ccggactggt cgccggtgcg
     6421 ccgcttcaag ggctatgccg cctggggtga accacagggg gcgggccgca actcactgtc
     6481 gtacaggcat atgggcgcct gcggctgtgc gaacgcctgc actgtgcatg ggcatcggca
     6541 tctggcggcg tggctgcccg atgcgcccgt gtccgacacc cagggcttct tcggcgccct
     6601 gggctgctct tgctgggcgg tctaggatca tggcgagctg ggaaaaacca tccgccgcga
     6661 gtccgggcgg ccgtccgctt gtcccgggag gcggggaaga cagccagcac gcctcgggcg
     6721 tagtgtgcct catcgcctac ctggggcttt gcgcggcata catccagggc gggctggaca
     6781 agctgatgga tttccacggc gccatcgggg aaatgacgta cttcggcttg tcgcccgcgc
     6841 cgctcttcgc agcactggtc atcttgctgg aactgctcgg ctcagcgatg gtgctgacgg
     6901 gcttctttcg ctggctcggc gcgcttgccc tgggcgcatt caccttgatg gcgaccttcc
     6961 tggccttgcg attctgggaa ctccccctgg gccaggaacg attcatggcc gccaattcct
     7021 tcttcgagca tcttggtctc gtcgggggat tcctgcttgt ggcgtggctc gacctgagga
     7081 gaaaatgaca tgaaactgaa cttgccgacc atcctgagct tcaacggcgg ctatgtcgat
     7141 accgccgggt tcctggccct gcaaggcctc ttcactgcgc acgtcaccgg caacttcgtc
     7201 acactcgggg cggccatcgc gcacggcagc tcgggtatca tcgccaaact gctggccctg
     7261 ccgatcttct gcgtcaccat cgtcatcgtt ctttatgcaa tgcggcacat atcgcactcg
     7321 cccaggcgct ccttgctgac gatgctcgtg ctcaagttcg tgttcctgac ggccggggcg
     7381 gcctgtgcca tccactacgg ctcgttcatc gactccgacg gctggccgct gattctcacc
     7441 ggcgcgctgc tgatcatcgc catggccatc cagaatgccg cccatcgcat ccacctgggg
     7501 tccgcgccgc cctccacgct catgaccgga accacgacgc aaatcatgat cgacctggca
     7561 aacctcttgc gcggggcatc gaacgaagcg ccggaagtag tcaaagcgcg ccttcggaga
     7621 ctgaccatcg cagtggtcgc gttcgcgctt ggctgtggct tcgcggcgct cttctacatc
     7681 gaattccaga tgtggtgctt catcgtgccg cccctttccg cccttacggg catcgccata
     7741 ctcatggcgg aaaaccaggg tccccagacg gactgaccgg ctggaactcg attcaagcgc
     7801 tccgtgctct tgcaacagcc acggatctgg tcgggcgatg aaccgagggt gagtcgcggc
     7861 ggcccgcccg atgccgtcac acgccgcccg atcgccaacg accacttcca tggcctgccg
     7921 acgacattcc gtcactgcat attcattaca atcacaaact gagtacttcc ctgcgcggcg
     7981 cttccgcccc ccgcgtgggg gccgcatccc tgcggcaacg cgtggccggc atgcgagccg
     8041 accgatgtca cctgtaccgc gccatcacat ttccagggcc catgacacca caacattctt
     8101 cctacaagag caagggtggc ccaggacggc tgctcaacgc cctgcggtac tccgtccagg
     8161 ggctgggggc cgcgctgcgc cacgaggcag cgttccgcca ggaactgctg gtggccgtgg
     8221 ttctcacgcc ggtagccttc tgggcaggcc gcacgcccac gcaggtgctg ctgctgatgg
     8281 gcgccatggt cttcgtgctg atcgtcgagg tgctgaactc cgcgctggaa gcgctggcgg
     8341 acagcatcac cacggacccc caccccatga taggacgggc caaggacctg ggcagcgccg
     8401 cagtcatgct gtcgatcgcg ttggcggtgg ccgtgtggct ggtcatattg ctgaccaacg
     8461 cgaacgcgcc atgatccgga accgccacgc caccttgcgg ccagccccgt catccccttc
     8521 aacaccccat tgaatgcacc catgaccttc attgaacaac tgaaccaatc ctggacgaat
     8581 tcgaattcac tgctgatggt cgggctggat ccggatccgg cccgctttcc caccgaactg
     8641 cgcgggcgag acgatgccgt cttcgaattc tgcaaggcca tcgtcgatgc caccgccgac
     8701 tacgtctgcg gcatcaagcc gcagattgcg tatttttccg cgcaccgcgc cgaagaccag
     8761 ttggaagccc tgtgccgcta tgttcacgac cgccatccgc ggcttcccat tgtgctggac
     8821 gccaagcgtg gcgacatcgg ctccacggcg gaacaatatg cgcgggaagc cttcgagcgc
     8881 taccaggccg acgcggtgac cgtgaacccc ttcatgggtt tcgactcgtt ggagccctat
     8941 ctcgcgcggc ccgacaaggg cgtcatcgta ctgtgccgca cgtcgaaccc gggcggatcg
     9001 gatctgcagt tcatcgagtc ggccgacggc acgcccctgt acctgcgggt ggccgaactg
     9061 gtggctggcc gctggaacac gaatggccag tgcgccctgg tggtgggcgc cacgtttccg
     9121 gaagaggtcg ccaaggtacg ccagcgcatc ggcgacatgc cattgttgat acctggcatc
     9181 ggcgcgcaag ggggagacgt gacagccacc gtggccgccg gccgcgacag ccagggcgcg
     9241 ggaatgatga tcaactcctc ccgcgcgatt ctgtacgcca gccagaacga cgattggcgc
     9301 gaggccgcca gccgcgtcgc gcgcgacacg cgcgacgcca tcaacgcggc cagggcgggc
     9361 ttaccctgac ccatgcgcag cgccgcgcgg cgagaaacgg gccccacaag cgccggagga
     9421 accggatcgt caaatgaact tggccgcggc atccagcgcg aacctgaccg cggcctcgcg
     9481 cacttgccga cgatcgcctt cgaagatatg ggtggcggca cgtgtgacca cgccctcgcc
     9541 cgcctgacgc gaaaacccga aacacaccat accgaccggc ttgcccggtg tggcgccgtc
     9601 gggcccggca atgccggtca cggcgatgga caggttggcg gtggccaccg caccgagcgc
     9661 acccgccgcc atttccatgg ctgtttcttc gctgaccgcg ccgaagcgtt ccagcgtatc
     9721 ggcgctgacg cgcaagtgcg ccatcttggc cgcgttgctg tacgtgatga acccgcgctc
     9781 gaaccactgg ctggacccgg ggatgtcggt caccgccgcg ctgagcagcc cgccggtgca
     9841 ggattccgcg cagaccagca tccagccgcg ccccagcagt ttctgcccaa gttcccgggc
     9901 caggtccgtc gaagcgatca agttggtcac agcatcccct gcaatcgaat gagaaccgcc
     9961 atcaccagca gcgtgtaggc cgccgcaacg atatcgtccc acatgacacc gaatccgttc
    10021 ttgatgtgcg cgtcgaagaa gtggatgggc ggcggtttga ggatatcaaa caccctgaac
    10081 acgatgaaag caagcaattg cgcgccgaac gaggccggaa ccaaccagag cacgagccag
    10141 aatgcaacaa tctcgtccaa gaccatgccg ccatagtcgg tgatgccaag ctcctggcca
    10201 gtgcggtggc aacaccaaca gcccgcgatg aacgccacca gcaatacgac gcccgcggcg
    10261 gcgtccggaa cacggccaag aatcaagaac cacagcagcc accccagcag cgttccccag
    10321 gtgccgggcc ccgggcggag cagaccgctg cccagcccga acgcggcgat gcgccacggg
    10381 gttttgaaga cccaggcggc attggggtcg gggcgggaag cctgggggtt cctggaatcg
    10441 tcgttgggca tggatgggta gccttctttt tttcgcgtca ggaggaaaaa tggtcgaagc
    10501 ccgcggaatg ataaggggca agccccccat ccgcactctg gactgcaagc ccggcgtgtt
    10561 cgacgatgcg cccgacccgg gtcacgcgcg tcgacgccgc cagcgccaat cgtgcaatga
    10621 tgtcgcgttt gtcggatggc gcggtgaaac acaactggta cacgtcccct ccatccagag
    10681 ccgcttgccg acgcaggccc gaatccagcc cgtccagacc tggatcgatc ggaagggccg
    10741 ccagatcgag ctcggcgccg caatggctgg cctccagaat gtggcccagg tcttgcgcca
    10801 aaccatcgga aatgtccagg gcggcatgcg ccacaccggc cagggcctgg gccagccgca
    10861 ggggcggttg cggccgttcc agcgcggggc gcaacgccgc cagccggccc ggatccagcg
    10921 gcagcttacc ctgcaacaac cgcaacgcga tgtcggccgc ccccagcgtg ccggaaatcc
    10981 agatgtcgtc gccgacccgg gcggcgtcac ggcgtagggc gcgagccggg tcgacctggc
    11041 catagacggt gacactgatg accacccccc atgggctgcg agtggtgtct ccgcccacaa
    11101 gtggacaacc cgcctcatcg gccagggaat gaaacccttc ggaaaacgcc cgcagccagg
    11161 gttcatcaac cttgggcacg gacaacccca gcacgcaggc cagcggcttg gcacccatcg
    11221 cggccagatc ggacagattc accgccagcg ccttgtgccc gagattgcca gggtcggtgc
    11281 caggcaggaa atgacggcct tcgagcaaca gatcggtgct ggtcgcgagc cgcatgcccg
    11341 gttccagcgg cagcagcgcg caatcgtcgc ccacccccag aaacccgggt ggcgcggggc
    11401 gcgaaaagaa tcgggcgatg agatcgaatt cgctgctcaa ggcgcccccg gaacgggtgt
    11461 caacgcctgg acgccgcccc gacctcggcg gaccggacat cggcggccac tttgtcgagc
    11521 acgccgttga cgaatttgaa cccgtctgtg ccaccgaacg atttggccaa ttcgaccgct
    11581 tcgttgatgg cgaccctata gggcacctcg acatggaaga ccagttcgta gcagccgatg
    11641 agaaggatgc tgtgttcgat gggtgagagt tcggccaggg gacggtctat gtagggcatg
    11701 aaacgttcac gcagcccttc ggcctcgcgg aacacgccat gcagcagcgt tttgtaccac
    11761 tcggcatcag cctcggagaa ttcctcgtcg tcgcggatgt gcgcatcgat ttcgccggtt
    11821 tccgccatgc cgcccccacg cagcagccag gaatagatgc cctgcaaggc gaattcacgc
    11881 gcccgccgac gggcgctacg cgtgttggcg cgggacgcgg ccgggccgcg cgaaggttta
    11941 tttgcggtca aggtggcctt cctcagcctg atcgtcgtcc tcgtcgtcgt cgagttcgtc
    12001 gtcatcgtcg tcgtcttctt cggattcggg ctcgagcgcg gcgaccaggt tcgccatttc
    12061 gacggcggcg gcggcgcagt cacgcccctt gtcggcggcg cggacctcgg cctgttcgtc
    12121 ggtgtcagtg gtgagcacgc cattggccac cggaatgccg gtttccagcg acacccggct
    12181 gatggccgac gcactttcgt tgctgacgag ctcgaagtgg taggtttcac cgcggatcac
    12241 ggcgcccaag gcgatgagcg cgtcgaattc aaaggtttcg gccatttgcg ccagcgtgac
    12301 acccaattcg agcgcaccag gaacggacac gaccatgaca tcgcgttcgt cgacccccag
    12361 gcgagccagt tcagccaggc aggcgtcgag ctcggccaag ccgatgtctt cgttgaaacg
    12421 cgcacggaca acgcctatgt ggagaccttc tccgttgaga tcgggcgaca gggtgtaagg
    12481 attcattgtc ggtggctccg tcaaggttgt ggggtgggtt cgctatcgta acccgtaatc
    12541 gtcagcgaga aacccgccat gctgggcatt ttcctgggcc gggcaagcag gcgcgccttg
    12601 ccgacgttca gatcgcgcag gatctgggcc ccgatgccat acgtcttgag atcgaacttg
    12661 ggcgcttcga ccgagcgttc cggcgcggcc caggactcga attggccgaa cacgagatcc
    12721 gccgaaccct ggcagttcat cagcaccacg acgcccgaag gcaccgcagc gatcgccttc
    12781 agcgcgctgg acacgcccca actgtggccg gaagaatcgt cgcacaacac atcgagcatg
    12841 ctggtgggtt cgtgaacccg caccaatgtc tcggtatcgg gcgaaatgtc gccatgcacc
    12901 aaggccagat gtggcgcgcc ggaggccagg tcgcgatagg ccaccgcctg gaattccccc
    12961 caaggcgtgc ggaccatgcg cgacgacacg cgttcgacca tggattcgtg ctcgctccgg
    13021 tactggatga gctcggcgat ggtgccgatt ttcaggccgt gccgctgcgc gaagagcttg
    13081 aggtccggca ggcgcgccat ggtgccgtcc gggttgagca cctcgcagat cacggccgcc
    13141 ggcgtcaggc ccgccagacg ggtcaggtcg cagcccgcct cggtgtgacc ggcccgcacc
    13201 agcacgccac cgcgcacggc ccgcaccggg aaaatatgcc ccggctgcac gatgtcggcg
    13261 ggccgggcgt cgcgcgccac ggcggcctgg atggtgcggg cgcgatcagc ggcggaaatc
    13321 ccggtgccca ccccttcggc ggcttctatg gacaaggtga aattcgtgcc gaagcgcgag
    13381 ccattgctgg acgccatgag cgacaggccc agttgccggc aacgctcttc ggtgagggtc
    13441 aggcacacca ggccgcgcgc gtgtgtgacc atgaaattga tggcgtcggg cgtgacgtaa
    13501 tcggccgcca gcaccagatc gccctcgttc tcgcgatctt cttcgtcgac cagaatcacc
    13561 acgcgacccg cgcgcaactc ggcgacgatc tcggaaacgg gggatatgtc gaaagactcc
    13621 ggcagaatgc cggaggacga ggcggcgtcg ctcatgatgt gtggctcggg aaaaggaaca
    13681 ggcccgattt taacgcgcac ctgtcggcac ggggttggtt atagtacggc tatcgttcca
    13741 tccaacccac cccctccagc ctcttcacgc ccccatgcca gaacccagaa cgctcgagaa
    13801 cgacggtcgc cgcggcatcc agtccgtgga aacaggcttt ccgctgctgg ccgccctggt
    13861 tgaagccggc accgctctca cactgcgtga tctctcggcg cgggccggca tggcgtccgc
    13921 caaggcgcac ccctatctgg tgagcttcgt gcgcgtggga ctggttcagc aggacgccac
    13981 caccggccgc tacgaactgg gtccgttcgc gctgcaaatg gggctggtga gcctgcagcg
    14041 gctcgacccg gtgcgcgtcg ccctgccgtg cgtgaacagg ttggcggggg aaatcggcca
    14101 cacgctcgcc atcgcggtac tgggctcgca cggccccacc atgatccaca tctgcgaggc
    14161 cagctacccc gtgcacgtca acatgcgcaa aggcacggtc atgtccatgc tgcacaccgc
    14221 cacggggcgt gtgttcgccg cttggctgcc gccccgcatc gcgcagcact acatcgaacg
    14281 ggaagagggg gaccaggcgg tggtcaccag cgtgggccag ccacccgtgg atccgaccgc
    14341 gtttgaggcg cagttggccg aaatccgacg ccggggaatc gagcgcacgc tgggcaaccc
    14401 gctgccaggc atcgatgcgt tttccgtgcc ggtgttcgac cattccggcg gcgtcaccct
    14461 cgcgctcacc agcctggggc cgagctcgtt gttcgattcg cgctgggatg gctcgatcgc
    14521 ccggtcgctc acggcctgtg cgcgcgacat ctcgaccagc ctgggctatc gggcgccggc
    14581 ctgagccctg tggggggccc tctgaacaag tcgagcggaa agcaggcgct gtggattggg
    14641 tccagagggt ccctagggct cttctcccac cagcgccgtc gcgaactcgc gcgccacgaa
    14701 gggctgcaga tcatccagcc cctcgcccac gcctatccag tagaccggca ccgggcgcac
    14761 cccctggcta ccagccgcca cggcggccag ggccccgccc ttggcggtgc catccagctt
    14821 ggtgaccacc agtccggtga ggttgatggc ggcatcgaac gccttgatct gcgagatggc
    14881 gttctgccct gtgttgccat cgatgaccag caacacctcg tggggcgcgg ccgcgtccgc
    14941 cttgccgatc acgcggcgta tcttcttgag ttcttccatg agatgcaact gtgtgggcag
    15001 gcgtccggcc gtatcgatca tgacgacacc cgcctcgcgc gccctgcccg cgttgacggc
    15061 atcgaatgcg accgcggcgg ggtccccgcc ctcctgggcg atgactgtca cattgttgcg
    15121 ggcgccccat gccatgagtt gttcgcgcgc cgcggcgcgg aaggtgtcgc cagcggccag
    15181 cagcacactg gcaccctgac gctggaacgc gttggcgagc ttgccgatcg acgtggtctt
    15241 ccccgccccg ttgacacccg cgatcatcac gatcagcggc gcaacgctgc ccagcgggaa
    15301 agacttctcc agaggggcga ggtgttccgc gagaatgtct ctcagcgcca accggacctg
    15361 tgaggcatcg gatagccgct ctttgcgtac gcgcgcgcgc agtgccgtca gcaactgctc
    15421 ggtggcggcc acgccggcat cggccatgat cagcgcggtc tcgagctctt caaagagggc
    15481 ttcatcaacc ttgacgccca cgaacaaacc accgatgctc tggccggtgc gtgacaggcc
    15541 gctgcgcaat cgtgacagcc aggatttacc ggcggcaggg gcggccacgg ccgcgctcgt
    15601 ctctggcggc acaaccggtt gagacaacgc catgtcgtgc gcgtcggaga cggatcctgt
    15661 ggagccatcc gcagccgcgt gggcaccgga caactcaggt gcagcagtgt cttcggaagg
    15721 ggcacgggca tgttccgaac cgctgctatc ggactccgct gcgcgcgttc cggcagcggc
    15781 gtcatcacca gtcggagcac ccggcgcggc cgggtcagcc gcctggtctt ccccgcccgg
    15841 acgcggcaca ttcttttttt tcaggaaact gaacataaac tctaggcttt acactaattc
    15901 ttcgaagagg cagaccaggg ccgaggcctg ggtcgaaaca gccgtaaccc ccgtcttgcc
    15961 atgaaaaagc atttactccg tattgtaggc ggtgattacc gccgcacgcc catacccgtg
    16021 gtcgacgccg agggcctgcg gcccacgccg gatcgcgtcc gcgaaactct ttacaactgg
    16081 ttgcatcatt tctggggcgg cgggttttcc gacaagcgtg tcctggacct gttcgcgggc
    16141 acgggcgccc tggggttcga ggcggcctcc cgtggcgcgg cgcacgtgca aatggtcgag
    16201 gccaatcccg aggcggtggc cgccctgcgc cagttgcgca cccggctggg cgcgagccag
    16261 gtgcgcatcc acgccggcga cgcccgaacc gttctggcgc ggctcaacgc ctcaccgttc
    16321 gacctcgtgt tgctcgaccc ccctttcggg ctggactggc tcgacaaact gtggcccgca
    16381 ttgcccgcac tgatggcgcc cgacgcgctg ctgtatgtgg aatcggaaac accggttgcc
    16441 ccgcccctcg cctttgaaat cgtccgacag ggcaaggccg gagcggtgca tttccatctg
    16501 ttgcgatttg ctgcatcgca aaaaaccggg aataattcca catcggaatc cactcaaccc
    16561 ggcgaccaat cagggcatca tgcctcggag ccctcccaac aaccggagcc agaatgatca
    16621 ttgccgtcta ccctggaaca ttcgacccgc tcacccgggg ccacgaagac ctggtgcgtc
    16681 gcgcggccag cctgttcgac cacctcgtcg tgggcgtggc ccacagccgc aacaagaagc
    16741 cctttttctc cgtcgaggaa cgcgtggaaa tcgcccgcga agtcctgggt cactacccca
    16801 acgtcgaggt gaaaagcttt gcgggcctgt tgaaggattt cgtgcgcgag cagaacggcc
    16861 gcgtcatcgt gcgcggcttg cgcgcggtgt ccgacttcga atacgagttc cagatggcgg
    16921 gcatgaaccg ccatctgctg cccgaagtcg aaaccctgtt catgacaccc tcggatcagt
    16981 accaattcat ctcgggcacc atcgtgcgcg aaatcgcgat actgggcggc gacgtcggaa
    17041 aattcgtgtt tccgtcggtg gagcgctggc tgcacagcaa agccaaggcc ctacgcgaag
    17101 cgggcgagca ggaatgacgc gtgtgctgtc tggttcatcc gggtttcaat gcccggacca
    17161 accagcgcat tgatcagcgg cggcgaacga aggccgcgcg ccggctacaa caccggccag
    17221 tccaccttgc ccgcatcggg gtgcaccttc atcaggcggc acggcgcctt caggtagttc
    17281 gtcagccagg tggctgccag atccccctcg tccaccacat cgaccttctg tgtgccgatg
    17341 gtggccaccc ggcgcacact atcgtcgtct tcgatgacgt cgagcggaat atccatgcgc
    17401 agcatgccgg gcgccttgat ggccaaatag ccgaaacgga tggcgggcgc gatctcaccc
    17461 agtagaggaa actcgtcccg cgtgaccagc cgcccgttct cgtcgacgac cagccagcgc
    17521 ttgtcgtaat ccgccgcatc cggatggtcg cagccagcac aagcggcaat ggggaaatac
    17581 atgtcgttgg gtttcatttt cctaggagtc ctttgagggc gttgccaatg ctcttgaccg
    17641 tgctcgacgg ttcgggtctg ggagcgggtt tcgattcagg agaggccggg gacgccgctg
    17701 gcgccggccc tcccccgacg cccttggaca gcagatcaag caacccccgt tgcacggcct
    17761 gcttgaccgc gtggctgccg atttccttcc actgcacctg gtaacctggc ttgctgaacg
    17821 gccccgcgat gcgcaccggc acggtcaccc cttccagttc ctcgaggcca ccctgcccct
    17881 tcgccgagcc cacgacccga gccttcacca tgatatccaa ctgcttgttg accagatcga
    17941 tggacgaggg ctcgccctcg gtgatgcgca gcatcggcgc catgatggac aggttcttga
    18001 gcgtgccctg tccctgactg aacaccaggc tcgcgtccag cgaggtgaag tcggtcttgc
    18061 gcgcggcgtc gaaagtcgtg gcaaggctgg atgtctggcc attgagcacg ttcttcaccg
    18121 cctggctgac ttcccgtagt gtctgagcca cgttgatgcc ggtgatggcc ccatcgtgta
    18181 cctgcgcctt gaccgtgccc gtcaagcccg cgacacaggc atcggccgtg gttcccgtcg
    18241 tacgcagatc gacgttcagt gaacccagcc ctgatagccg accaggctgg cccagcgcaa
    18301 gcaacatcgg caacaccgat acattgcgca gcgacgcctt ggtcgccaga tcgccattcg
    18361 aggtggcggt gaatccaccg gtgagcgtgc cctggtaaag atcggccgtg acccgggtca
    18421 cgtcgagcgt gcctttggcg gcgcgcacag cggcgctgaa cttcttcgcc tcgaactggc
    18481 cgaccttcag gtcgccgatc ttgatggtgc ccgtgacatt ggccgaatcg aggaacttca
    18541 ggtcgatcgg ctcggatggg gcaggggccg cttgcgtcgc cggccccgct ttcccggacg
    18601 tcgagggtgc cgccttcttg gccgccggtt tgcctggcgc ggccgtcggt ttggcgtccg
    18661 gcttgccggt aacgggcggg aagagcttgt tgaaatccag agagtcggcg ttcaaatcga
    18721 aagccacggc cggatcattc aggtgcgtgg ccttgagctt gaaatccagt ttactgccgt
    18781 tcagaacggc gtcgatctcg ctgacgatct gatccttggc cagatcggcg ctgaggctgc
    18841 caatcatggg gaaatcaaag ctgccacctg gcaacgcggc atcctggatc ttcatgtcac
    18901 ccttgatggc cgacaggccg ccttgctcag acaggaaatt ccagcttccc ggcgatgaca
    18961 gattcaactg caccaggcgg tcaccctgct tgaggccgct gtcgaccttg agctccttga
    19021 agctcaggtt gtacgcgttg ccgtcgattc cggccaatcc cagcgcgacc cccaacacct
    19081 cgttgtcact ggtcagcttg accgtgcccg ccacgggctc gcccttggcg gaatccgggg
    19141 aaatggaaag gctgggggcg tcgaacgcca cgtcgaaagc gcgatccggc agattaccct
    19201 tggcgcgcag cgccagcttc tgtacctgga attcccagcg gctgcgatcc acgcggagcc
    19261 gcggcacggc cagccgaacg tcgagattct tgatctgcgg cacgcccagc ttgcttgggt
    19321 gcacgttccc ttgcacggac aactcgatgt tgctgacgtc gagcatccgc gagtacgcgt
    19381 cataagcgag attgccttgc agggtcgcgg acttcgcatc cagagacccc aacagcccct
    19441 tgagctggag attcagcttc tgcgccgagt aggtcctggc ctcgggattg aacgtcacca
    19501 ccgcctggcc ttgaatgtcg gccttggcca ccggatagtc gccgatgagt ttgcctttga
    19561 gcgccacatc gaacggctgg ccgaacgtca cgcgccccgt gttcacatcc aggtgctgaa
    19621 cccgcgcgga tgtcccggtg acggcgtcga aatagtggat ctcccccccc ttgagatcca
    19681 gcccggcgat gtcgatctgg aaatcggtgc gcgcggcatg acgatcggcg atcgcggcag
    19741 gcagcgacac gagctgctgc gcggccccgc ccgccagggc gatgggcgtg gcatgggcga
    19801 cagcccccat cttgggagcg gattggctcg cggcatacgg cggtcggctc ccgagcaagt
    19861 cgtcaaaatt gaaacggcgg tgcttgtcgc gggcaatcca ggccttgaaa cccgatacgg
    19921 cgacatggtc gacgaccagc cgatttgaaa tcaacggcca tatcgccacc gcgaaacgcg
    19981 cactgtctat cgaagcgaag gtgtcgtcgc tgttgacgtc cgacagcgac acatcatgga
    20041 ccgacaggcc gatacgggga aagagagaca gttccagcgg ccccttgatg gccagggtac
    20101 ggtggtaacg gctatagacg aggcgctcga gcttggcttt gtacgcgttc ggatcaaagg
    20161 tcagcaggaa gatggctatc ccgaccaatg tggcgatcac cagtacgacg atcgcgatca
    20221 aaattcgctt gaaccaaact ttcatgtgct gtctcagact ccggaaacga cttcagccgg
    20281 ctcgcgacgc aggagcgtct caggtaaccg acacgtcgaa tgctaaccca agtcttatgg
    20341 ctgtatggaa attcccggac gatttcatgc cccgggcctg acaaaatgtc ggttttcatc
    20401 aaatggaaaa acggggaacg gcaccgtccc gggagggagg atggccgcct gacggcggcc
    20461 ccgcaatacg gagaaggcca aacggccgtg agacggccgg gccggaaacg ccacgtggct
    20521 acagcttgcc cagcaagcca gacaccgccc ccatgagcga cgacgcatcc aggctttgcc
    20581 cgtcgggcac tgagccgttg ggcgtggcct gatccaccac atgaggcaac agcgagctca
    20641 gtccggccag caccgagctc ttgtcctggt tcgtgctctg cgccagatta ttgacgacat
    20701 cgtcacccag gaccgactgc acctggtcac tggacacgga ctggttggcc ccggttccga
    20761 cccatgacga tacgacgtca cccaggccac ccgcctggaa tttctgcacc aggccctgca
    20821 gcccccctgg gtaattgttg atttccgcga ccagcgccgg cagcagactg gccgcctgcc
    20881 cggaatttgc gttgcccccc accgcggaca tgacagaatc gagaagtccc atgatagacg
    20941 ctccttggtt attggaattc gagtgtaccg cccagatggc gactggaaga aaaaattgac
    21001 gaatcttgcc gtgcccagcc atccgcgctg atgtcggcca tcatcgcaca cccacacacc
    21061 gggcccggta acttcatgta tagtgatcgc accaaaaaaa acaccggcac ggaggagcgc
    21121 catgctgata gggatcccgc gcgaaagcct tataggtgaa acgcgcgtcg ccgccacgcc
    21181 cgagaccgtc aagaagtacg tcaaggccgg gcacaccctc gtcatcgaac aaggcgccgg
    21241 cgaggcggcc cgctatcccg accaagcctt cacggacgcc ggcgccgaac tcggcgatgg
    21301 gtcccgcgca ctgtcttgcg acctggtgct caaggtacgc tcacccgaca gcagcgaact
    21361 cggccgcatg aaacccggcg ccacgctcgt cggcatgctc gatccattca accgcgaagg
    21421 ccttgaagcc ttcgccgccg cgggtatctc ggcgttcgcc ctggaagccg ccccgcgcat
    21481 cacgcgcgcg caaagcctgg acgtgctgtc ctcgcaagcc aatctggcgg gctacaaggc
    21541 tgtgctgctg gctgccaacc attaccagcg tctgttaccc atgatgatga ccgccgccgg
    21601 cacgctcaag gcgggccgtg tggtcattct gggagccggg gtggccgggc tgcaggccat
    21661 cgccaccgcg cggcgcctgg gcgccatggt cgaggcgtcc gacgtccggc cggccgccaa
    21721 ggagcagatc gaatccctgg gcgcgaaatt catcgacgtc ccctttgaaa ccgacgaaga
    21781 acgcgacatc gcgcaagggg tgggcggcta tgcgcggccc atgcccgccg cctggatggc
    21841 gcgccaggcg gtgctcgtgg ccgaacgctg ccgccaggcc gacatcgtca tcagcaccgc
    21901 cctcataccc gggcgcccgg cgccggtgct catcaccgaa gacacggtca agagcatgaa
    21961 gcccggctcg gtcatcgtcg atctcgccgt cgagcgcggc ggcaactgcc ccttgtccga
    22021 aaaagacaaa gtcgtcgaaa agcacggcgt catcctggtg ggccacagca acctgcccgc
    22081 gctggtgccc accgatgcgt cggccttgta cgcccgcaat gtgcaggatt tcctgaaact
    22141 gatcatcagc cccgatggcg ggctggccat tccgcaagac gacgaaatcg tcaccgcctg
    22201 cctggtctgc gccggtggca aggtactgag gagcgcctga tggaagccgt gaaccccacc
    22261 ctgatcaatt tcatcatctt cgtgctggcc gtctacgtgg gctaccacgt ggtctggaac
    22321 gtcacaccgg ccctgcacac cccgctcatg gccgtcacca atgccatctc ggccatcgtc
    22381 atcgtcggcg ccatgctggc ggccggcctc accgacggct acctgggccg cttcatgggt
    22441 gtgttcgcgg tggcgctggc ggccgtcaat gtcttcggcg gcttcctggt cacgcgccgc
    22501 atgctggaaa tgttcaagaa gaaagaccgc aagacggggg gccgggcatg atctccacca
    22561 atctggtcac cctgctctac ctgatcgcct cggtctgttt catccaggcc ctgaagggcc
    22621 tgtcgagccc caccacctcc cggcgcggca atgcgttcgg catggtcggc atggccatcg
    22681 ccgtgctcac caccgcggcc ctcatcgtcg ggctggccac ccagggcacc gctgttatcg
    22741 gcctgggttg gatcatcctg gggctgctca ttggcggcac gttcggcacc ttcctggcca
    22801 agcgggtcga aatgaccaaa atgcccgagc tcatcgcctt catgcacagc atgatcggcc
    22861 tggcggcggt cgccatcgcc atcgccgtca tctccgaacc ccacgccttc aacatcgccc
    22921 tgcccggcca gccgctgccc accggcaacc ggttcgaact cgtcatcggc acgttcatcg
    22981 gcgccatcac cttctccggc tctgtcatcg ccttcggcaa gctgtcgggc acctacaagt
    23041 tccgcctgtt caagggcgcg cccgtggtgt tccagggcca gcacctgctc aacctggtgc
    23101 tcgtcatcgt catgctggcc ttcggcgccg gctttatcgc cacccaatcg tggctgccct
    23161 acgtcatcat gctgctgctc gccttcgccc tgggcgtgct catcatcatc cccatcggcg
    23221 gcgccgacat gccggtcgtc atctccatgc tcaacagcta ttcggggtgg gccgcggccg
    23281 gcatcgggtt ctcgctgaac aaccccatgc tcatcatcgc gggttcgctg gtgggctcat
    23341 cgggcgcgat cctgtcctac atcatgtgca aggccatgaa ccgctcgttc ttcaacgtca
    23401 tcctcggtgg cttcggcaat gaaggcgggg cggcgcaaac cggcgagcac gcacaacgca
    23461 acgtaaagtc tggcagcccc gaagacgccg cgttcctcat gtccaacgcc gagaccgtca
    23521 ccatcgttcc cggctatggc ctggccgtcg cgcgcgccca gcacgccctg aaagagctca
    23581 ctgaaaaact ctcgatcgcc ggcgtcacca tcaaatacgc catccacccc gtggcaggcc
    23641 gcatgccggg ccacatgaac gtcctcctgg ccgaagccga cgtcccctac gaccaggtct
    23701 tcgaaatgga ggatatcaac ggcgaattcc cccagaccga cgtcgtgctc gtcctgggcg
    23761 ccaacgacgt cgtcaacccc gccgccaaga acgacccgaa atcccccatc gcgggcatgc
    23821 ctatcctcga agcctacaag gcccgcaccg tcatcgtgaa caagcgcacc atggccgccg
    23881 gctacgccgg cctcgacaac gagctcttct acatggacaa gaccatgatg gtcttcggcg
    23941 acgccaagaa agtcatcgag gacatgatca aggcgctgga atagccccac gccccgccat
    24001 gaggggagtg tcagccaaac tgaatcccct gcgccagcgg caactcccgt gaataattga
    24061 ccgtattcgt cgcccgtcgc atataagtct tccaggcatc cgacccggat tcccgtccgc
    24121 cgccggtttc cttctcgccc ccgaacgccc cgccgatctc ggccccggac gtaccgatgt
    24181 tcacgttcgc gatcccgcag tccgaacctg acaccgacag gaacgtctcc gcctcacgga
    24241 gatccatcgt gaacaccgcc gacgacaggc cctgcggcac cgcgttctgc aaacgcaacg
    24301 cctcgtcgaa atcccgatag cgcatcacat acagaatcgg cgcgaacgtc tcctgacaca
    24361 ccacgtccgt ctgccccggc atttcaacca gcgccggcga cacataccgc gcggcgggaa
    24421 actccgcggc cagcatgcgc tcgccaccga agacatttcc tccctgctcg cgcgccagcg
    24481 ccagcgcctt ttgcatggct tcgaacgccg ccacgtcgat cagcggcccc accagcgtat
    24541 cgggctccag gggatttccc acgcgcgcgc tggcatacgc cgtctttaga cgcgccacca
    24601 gatcgtccgc cacgctttca tggacgatca atcgccgcgt cgtcgtgcaa cgctgccccg
    24661 ccgtgccgat ggcaccgaac aagatgccgc gcaccgccag gccaagatct gccgaagccc
    24721 cgacaatgat ggcattgttg ccccccagtt ccagcaacga ccgcccaaac cgcgccgcca
    24781 cgcgcggccc cacctggcgc cccatgcgcg tcgagccagt cgccgacagc agcgccacgc
    24841 gctcgtcgtc gaccagcgcc tcgcccacgt cgcgcccgcc aatcagtacc tgcgacaagc
    24901 cctgcggcgc atcgccgaac ttcgccagcg cgcgctcgaa cagcgcctgg cacgccagcg
    24961 cggtcaacgg cgttttttcc gatggcttcc acaccaccgc gtttccacac acgatggcca
    25021 gcgccgtatt ccaggaccac accgcgacgg ggaaattgaa cgccgaaatg acgcccacca
    25081 cccccagcgg atgccaggtc tccatcatgc gatggcccgg tcgttcggaa gcgatggtca
    25141 ggccgtacaa ctggcgggaa aggccgagcg cgaagtcaca gatgtcgatc atttcctgga
    25201 cctcgcccat gccctcagcc aggatcttgc cggcctcgat cgacaccagc cgacccaatg
    25261 ccgccttgct ggcgcgcagc tcatggccga agaggcggat caattcgccc cgccggggcg
    25321 ccggaaccgt cctccaggcc aggaaggcgt ttttggcttc gccaatggct gcgatcgtgt
    25381 cgtcgcgcgc atgctcgcgc aagcgcgcga tttccgcgcc atcgatcggg gaatggacgg
    25441 caagcgtgcc gccctgcaac cttgaagggg ccacacccag gccttccagc aattccgaag
    25501 tagtcatgtc tcttctgctc gcaatgaatt tttgattgtc acgggatatg ctgaacacgc
    25561 cgccgcgccg cacgcatccc cggtacggaa cgcctgcttg ccgctggccc tgttcagatg
    25621 agtctcatcg cagaatagca taataattcc tatacgaaaa tttattatca aaagggaaat
    25681 cacctatgga agcctggcca ccgcccaaca acaatggggg catcgcatga accgcaaaca
    25741 aaccggagca ccgcgcaaac atgcctttcg atacttatga cgtgatcatc gcgggcggcg
    25801 cggccgccgg cagcgccgcg gcctatttcc tgacggcgaa ccccgacttc aacggcaagg
    25861 tgctggtcgt cgagaaggac ttcacttatc aacactgcgc caccaccctc tcggccgcct
    25921 cgatacgaca tcagttctcc acgccggaga acatccggat gtcgcaattc ggcacacaat
    25981 tcctgcgcgg tttcggcgcc acgatggctg tggatggcat cgaaccggac gccgcctttc
    26041 acgaagccgg atacctgttc ctggccacct ccgcaggcat gaatgtgctt cgcgagaacc
    26101 atgccaccca gaaggatctg ggtgtcgatg tggctctgct gaatcccacc cagctcacgc
    26161 aacgctaccc ctggctcaac acttcggacc tggccgccgg ctccctgggt ctgtccggcg
    26221 aaggctggct ggatgcttat ggcatgatgc agggcctgcg caggaaagcc atcgcgcaag
    26281 gtgcccgata tatcgagaac acggtgacgg gcgtgcggca cgagggtcga cgcatcgtcg
    26341 gcgtgaccct gtccgatggc acccatctcg attgcggcac actcatcaac accgccggca
    26401 ccggcgcccc cgcactggcc cggctcgccg gcatcgcgct acccgtggag gcgcgcaagc
    26461 gctgcgtatt ccacttccaa tgccccgccc ccctgcccga ctgcccgatg gtcatcgacc
    26521 caagtggcgc ctacttccgg ccagagggca acgggttcat tggcggcatc tcgcccgacg
    26581 aggcgcatga tcccgaatgc ttcgatttcg acgtccagca tgaagtcttc gaagacgtcc
    26641 tctggccgct tctggcacaa cgcgttccgg ccttcgaggc cttgcgccga ctcaatgcct
    26701 gggcgggcca ttacgacatg aacaccgtcg accacaacgt catcatcggc gcccaccccg
    26761 acatcgacaa catgctgttc gccaatggct tcagcggcca cggcatgcaa caatcgcccg
    26821 ctgtggggcg cgcactgtcc gaactggtga cctggggcgg attccgcacg ctggatctgt
    26881 cgcgcctggg atgggcgcgc atcctggagc accgcccgat cgtggaaaag aacatcgtct
    26941 gatgcgcggc atgtcacact ctgacggagg ccgtcgcgca agccgcccgc tcgacccacg
    27001 caggcccgac aacactactg gataccccgc ttgaaccgca caccctcaac ctcgaatccc
    27061 cccgcgctgc ccgaacggct gcgatcgctg cggcagtcgc gcggctggac cctggacagg
    27121 accggcaagg ccagtggcgt ggccgcctcc acgctttcca agatcgagaa cggcctgatg
    27181 tcgcccactt acgacgtact gctcaagctg gcggacggct tggggctgga cgtggcggag
    27241 ctattcgcgc cggcccgggc ccacatgggc gccggacgct gcagcgtaga gcgccaggat
    27301 ggcggcgaaa tccatgaaac cccgttgtat cgccacagac tgctgtgctc acagttgtcg
    27361 cacaagcgca tgatgccttt cgtcaccacc atcaaggccc actccctggc cgagaacgag
    27421 ggatggagcc gccacgacgg cgaagaattc gtctacgtgc tgaagggcgc ggtggaactg
    27481 cacaccgagt attaccagcc caccaccctg aaggctggag actgtttcta catagacagc
    27541 cgcatgcgtc accgtgtcgt caatcgcggc aagatcgacg cgaaggtcat ctgggtcagc
    27601 acccagcagg gcgtgccagg acagcacgcg ctcaatcacg aaggccacgc cgactgaccg
    27661 cgcgcccccc ggagaatacg ccatgacaac cttccaaaca ctttccgtct tcaacgccga
    27721 agaaaccgcg aacctgttgc cgtttccgga actggtcacg gccatcgcca ctgccagcgc
    27781 gcaatgcgcg cgcggcgaca ttgacgcccc ggaacgccaa gtggtgccgc tggcgcgcgg
    27841 cgccggcgtc atgctgtcca tgccggccac agccgaggac gtcgccatcc acaaactcgt
    27901 caacgtcgtg ccgggaaacc gcgcacgcgg cctgcccacc attcatggcg tcgtcacagc
    27961 ctatgatggc gcgacaggcc gggaactgtt tgtcctggac ggccccacgg tcacggcgcg
    28021 gcgcacggcc tccgtctcga tgctgggcct gaagacattc tgtgccaaac ctccccggcg
    28081 catcgcgctg atcggcaccg gcaaacaggc atccggccac gtacaggcca tcgccgcgtt
    28141 gtatccgggc atcgatctct gcatcgtcgg ccgcagccaa gccaaggcgc gggaattcgt
    28201 cgccgcgcac gacggctacc cactcacgtt ccatgccgcg accgaggcgc cgccggacat
    28261 cgatgccgcg ctgacgacca ccaccagcaa aacaccggtg taccacttgc ctgcgcgggc
    28321 ggggcgcctg gtcatcgggg taggcgcctt tgaggccacc gccgcggaaa tctcgccgga
    28381 aaccgtccag gcgagccaac tctacgtcga cgaccccgcc ggcgcgcacc aggaggccgg
    28441 tgacctgatt ctagccggcg tagactgggc ggccgtccgg gcgttggccg acgcgctggc
    28501 ccatggcgcg gacacggccc gccccatcgt gttcaagacc gtcgggtgcg cagcctggga
    28561 tctggccgcg gcaagatgtg ccatggccag attgcaaccc tgatatgaaa cacccgcttc
    28621 gggtatgccc ggtcggggca aacccaggtg gacgaaacgc ggcgatttgt ctaccataga
    28681 cacatcattt actttgattt acaaaatccc gtcatgtaca tcgccacgcc gggtctcctg
    28741 catgaccacg acgaccctat gctcatcacg gggcggcttg ccatcacatc actattgcgc
    28801 tccctgaaag aacgcaaagc cctggtcggc atgcatttgc gcggcagcac tctcaccgtc
    28861 atcaccgcca tcctccaggt ggacgagacc cggggccttt tcatcatcga tgccgcgcgc
    28921 gatgcgcaat tcaacaagcg cgccaccgac gccgaagata tccgcttcga atccgccctc
    28981 gatggcatcg gcctgaaatt ctcaggtgcc tatgcctcgc cgtgccacca cgaaggcaaa
    29041 ccggcgctgt gtcttgctct gccgaaatcg ttgcaacgca tacaacggcg cgaatcctat
    29101 cgtgtcgagg ttcccgtcag ccagccggcc cgctgcacgc tgacgccaac cgaccagaat
    29161 gggtcggttg ccaccgccat cacgctcgat ctgaaagaca tcagcctggg cggggtgtcg
    29221 ctcattgaca acctcaagca attacaagcc gaccaaggta gcctctaccc aaactgccag
    29281 atcgtgctgc ctgaagccgg caccatcact gccacactgc gcgtcgtgcg gatcctgaaa
    29341 tcaaccctgg aaaatgggaa aaccgtccgc cacctgggtt gtgcgttcgt cggtctgcca
    29401 aaccccatgt ccaccatcgt gcagcgttac gtgggcctgc tcgagcgcaa gctcagcgcc
    29461 aagcgctacg gaatcgacta accgccgtca cgccatgcgc cgcgcccccg cgacgcacaa
    29521 cgccgcgacc accatcacca cgatcatcgc cagggtatag ggttcaccca acagcgtcgc
    29581 cgccagcagc aagccgaaaa atggctgcag aagctgcagt tgcccaaccg tcgcgatgcc
    29641 gccaccggcc aggccgcggt accagaacac gaacccgatc agcatgctga aaagcgacac
    29701 gtagcccagc cccacccatg cctgcggcgg cacattggcg aacgtcgcgg gccatgccat
    29761 ccaaagcgcc accagcatca ccggcagcgc caccaccagc gcccagcaga tgacggccag
    29821 accgcccagg cgccgtgaca ggaccgcccc ctcggcatag cccagcccgc acacgagaat
    29881 cgcacccaac atcagccaag cgcctgtccg tgacgacggg gcctgatgac tgaacgcgta
    29941 tccgaccacg gcggccgcac ccagcaccgc gaacagccag aagccccacc ggggccgctc
    30001 tccgccccgg atcaccccga acaggctggt cgacaacggc agcacaccca cgaacacaac
    30061 ggactgcgcc gccgtgatgt gctgcaacgc cagcgccgac agaaagggaa agcccagcac
    30121 cacgcccagc gccacgacga caagggacat caggtcgcgc cgcacgggta tgccctccct
    30181 gcggaccacc aggagggcga tggccagcaa gcccgcgatg gacgcccgcg ccaccgtcac
    30241 gaaagtcggg tcgaggcctt gcaccgccac acgcgtggcg ggcagcgaac cactgaatat
    30301 cagcatgccg atgaaaccac tcacccaggc cgaagccgta gtcatgccac tcgtgatgtg
    30361 cattctgccc gcgccgctca tgttgttcac gccgttgggg ttgctcgtgc cgcttatgcc
    30421 gctcatgttc gttctccttg ccaagcgagt gtcccaggtg agccaccgca aggccagaga
    30481 cagtacaata caattcacaa gaactgttat ggtcacgcag gcagtacaga tgaacaccct
    30541 cgtcactggc gccacccgga tcgaacagac catggacgcg atacgccgtc gtatcgcgct
    30601 caggcaactg acgccgggcg cccgcgtacc ctccatccgc caattcgccg ccgacaccgg
    30661 ggtttccaag tccacggtgg tcgaagccta cgagcggctg gcggccgaag gcgtcatcca
    30721 cccccgccgg ggttcaggct tctatgtggc cggccacctg gcgccgctgg ctctttcaga
    30781 actggggccc aggctcgatc gcgaagtcga cccgctgtgg gtggcgcgcc agtccctgga
    30841 ctccggaacc ctggcgctca aacccggctg cggctggctc ccgaacgaat ggatgccgca
    30901 agacgatata cggcgggccc tgcgcgggat cgcgcgcgcg ccacacacgc cgctgaccga
    30961 ctacgccccc acgctggggc agccgggatt gcgccggctt ctcgcgcagc gtttgcaaga
    31021 acgcggaatg aacaccacac ctgcccagat cctgctgacc gactcgggta cccaggccat
    31081 agacctgctc tgccgcttcc tgatcgagcc cggcgacacc gtgctggtgg acgacccctg
    31141 ctatttcaat ttcctggcgt tgttgcgcgc ccatcaagtg cgcatcgtca gcgtgccctt
    31201 caccaccacc gggcccgacc tggaactgtt cgccgccacg ctcgccgaac accagccacg
    31261 cctctacatc accaactcgg ccctgcacaa cccaaccggc gcctcgttgt cgccactggc
    31321 ggcccatcgc atcctgaaac tcgccgaagt ccatgacctc gtcatcatcg aagatgacat
    31381 cttctcggac ttcgaatcgt caccctcgac acgcctcgcg gcgttcgacg ggctggaacg
    31441 cgtgatccag attggcagct tctccaagac actatcggcc tcggcgcgct gcggctacat
    31501 cgccgcgcga gcggactggg tggacagcct ggccgacctg cggatcgcca ccgcgttctc
    31561 gggctgcgcc ctgacgcaat ctctcgtgca ggcgctgctc gagggtggcg gataccgacg
    31621 ccacgtggaa acgcttcgac aacgcctggc cgacgccacc gcgccggtca tgacacggct
    31681 agaacgcctg gggatcaccc catgggccgc gccgcgcggc ggcatgttcc tgtggtgtcg
    31741 attgccacac gggctcgacg gcgcccgcct ggccggattg gccctggcgc aaggtctggt
    31801 gctggcgcca ggcaatgtct tcagcctgtc acagaccgcg agcggcttca tgcgcttcaa
    31861 tccgtcgcag atggccggcc cgcgcgtgtt cgaactgctt gaaaccgcca tgaaccaggc
    31921 cgctacaatg gcggacgaac cccgcgccgc cgcctagtgt cgtgtctcac aagctcgttc
    31981 gagcacctga cttcggcgcc tctttccatc cctcccggat acacagccca tggaaaatcc
    32041 tggcatcgac gaccagcaga catcgccttc cgcgcctgtt gtccagaacg ccttccaggg
    32101 cgacctgatg gcgcatgtcc aggcgctgcc cgatacctgg cggcaggcgt tcgacgacga
    32161 acagacgcaa ggcacgctgc acgacctggc cagctttcta gatgacgaac tcgcgcgcgg
    32221 catggagatc tttccgctgc ggccatttcg cgccttgctg gaaaccgacc ccgatgcggt
    32281 ccaggtggtc atcctgggcc aggaccccta tcatgggccg aaccaggcac aaggcatgtc
    32341 gttctcggtg cccgacccct gccccacacc gcccagcctg cgcaacatgt tcgccgaact
    32401 cgcgcgtgaa tacgaaggcc ggttcgaacg ccaacgcaac agcctgctgc gctgggcccg
    32461 tcagggggtg ctgttgctga acacgtcgct gtcggtcgag gcgcacaagc ccgcctcgca
    32521 tgccaaacgc ggctgggaaa ccatcaccga cgccatcgtc gcccgcgtgc tgcgcgagcc
    32581 gcgccccaag gttttcctgt tgtggggttc acacgcccag tccaaggaag ccctgctacg
    32641 cgaacatccg ccgcaagggc cggtgctggt gctcaaggcc aatcaccctt cccctttgtc
    32701 ggcactgcgc ccgccccgcc cgttcatcgg ttgcggacac ttccagaaag ccaacgaatg
    32761 gctgcaaggc cacggggaaa ccggcataga ctggcttcag aacaccacat cgccgcgata
    32821 gcaatggcca tcgcgaggat tcgcaacgcc gc
//
LOCUS       DASTNK010000079         8606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_796518, whole genome shotgun sequence.
ACCESSION   DASTNK010000079 DASTNK010000000
VERSION     DASTNK010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8606
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796518"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /gene="rhuM"
                     /locus_tag="VFR20_06245"
     CDS             complement(<1..811)
                     /gene="rhuM"
                     /locus_tag="VFR20_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HEU0230152.1"
                     /translation="MNKPRKPAVTQRPQSAIESGQTRGSEFLLYETEDGRTRVECRFV
                     DDSLWLSQALMTELFQTSKQNISLHVKNVLAEGELQTAATVKDYLTVQTEGSRQVRRK
                     VRLYNLDMILAVGYRVKSPRGTQFRRWATERLAEYLLKGFTLDDERLKNPPVGDSALP
                     DRFGELLARIRDIRASERRMYLRVREIFALAADYTPTLPETTAFFRIIQNKLHYAVSG
                     QTAAEIIRNRADHTRPDMGLTSTRKGQVQKADVSVAKNYLTEAEITELNRIV"
     gene            complement(804..2330)
                     /locus_tag="VFR20_06250"
     CDS             complement(804..2330)
                     /locus_tag="VFR20_06250"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEU0230153.1"
                     /translation="MNSSARQTAPVSQDDINAAVWGACDTFRGTVDPSIYKDFVLTML
                     FLKYVSDVWQDHYDTYKKQYGDHPELIEEMLKNERFVLPASASFHVLHTLRHSPGNGE
                     RIDKALHAIEEANITKLRDVFQDISFNSNKLGDEQQKNDILRHLLEDFAKPELDLRPS
                     RVGQLDVIGNAYEFLIKNFASTSGKKAGEFYTPPEVSSLMARLMAPQEGDDICDPTCG
                     SGSLLMKCGRLVRENTGSRKYALYGQEAIGGTWALAKMNMFLHGEDNHRIEWGDTIRN
                     PKLLDSTSSLKHFDIVVANPPFSLEKWGHEGADTDPFSRFRRGVPPRTKGDYAFILHM
                     IEIMKPGTGRMAVVVPHGVLFRGAAEGRIRRKLIEENLLDVVIGLPEKLFYGTGIPAA
                     VLVFRKNKADDKVLFIDASRDFEAGKNQNMLREADLRRILSTATARQSVDKYAYLATP
                     AEIAENDFNLNIPRYVDTFEEEAEIDLMAVRREREQLKAELAGLEAQMAVYLKELGYE
                     "
     gene            complement(2353..2958)
                     /locus_tag="VFR20_06255"
     CDS             complement(2353..2958)
                     /locus_tag="VFR20_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU0230154.1"
                     /translation="MTIDAFISTVGALASLKAGYPFRGSIDDLADGETLALQMKDVDP
                     DHGVNWRGVTRTSLTGRKQPDWLKAGDVLFVSKGARFYAVCIDEPPSPAVCSPHFFHL
                     EVAARAPLLPAFLAWQINQPPFQRQLQQAAEGSSQLSIRRPVLESLTLCLPSLADQRR
                     IVALASLASQERHAFHQLMHNREHQLQALAEDLAHAARATH"
     gene            complement(3141..3626)
                     /locus_tag="VFR20_06260"
     CDS             complement(3141..3626)
                     /locus_tag="VFR20_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230155.1"
                     /translation="MYHFTDGGLRNVWLVNGYKTSETPYGSAVSIQDVKGLTRAICKV
                     LVRKKSHLTGAEFRYLRLALLMSQSGLGRTLGRTEQAIASWEKTGHIPKFADTMMRFI
                     YARHENGDEQVKNIVEAVNETDRDIHIVMRDSKAGWSGSESTKRPEAQSSCDSEDLVP
                     V"
     gene            complement(3629..3934)
                     /locus_tag="VFR20_06265"
     CDS             complement(3629..3934)
                     /locus_tag="VFR20_06265"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HEU0230156.1"
                     /translation="MALNKAGYERRIHELARDSASITWTEHAKQQMRRRHITMSVVLD
                     VLQRGIIHKEPEPDIKTGHMKCTIERFCAGRPTAAVVALESESASSCFVVTAFLIGD"
     gene            complement(4294..4383)
                     /locus_tag="VFR20_06270"
     tRNA            complement(4294..4383)
                     /locus_tag="VFR20_06270"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4347..4349),aa:Ser,seq:cga)
     gene            4726..5451
                     /locus_tag="VFR20_06275"
     CDS             4726..5451
                     /locus_tag="VFR20_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0230157.1"
                     /translation="MAARIVQKILYQEVADRLRAMIQSSELQAGEWVDETRLTETLGI
                     SRTPLREALKVLVGEGLLRIEPRRGCFVNELSSRDLDDIFPLMAMLEGRCAYEAARKA
                     SEADLRHLEPLHRQLRTHATTGDVDQYYEANARIHEAIQALADNRWLSDLIDNLRKVI
                     SLSRHRSLAYAGRINESCAEHLAIFAALKAHDPDGAEALTRQHLMRQLDALHAMAEQE
                     SSTDGRSGGQGQPPALRQRGVRS"
     gene            5448..6791
                     /locus_tag="VFR20_06280"
     CDS             5448..6791
                     /locus_tag="VFR20_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050080 - malonyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase family protein"
                     /protein_id="HEU0230158.1"
                     /translation="MSSSDKPKDAQASDSLAADTQGQRVPVLPEAPTRGKKRPGDASG
                     LLARLSRWLERDQLPRESEIGTLYRAKLTDVAANKLAKQWGERYLQADGPERCSLLAG
                     LVQAGVGLEPRGDQGFRLFRRLYAQTDNLQMLVELRADMLRWRNQVAGLAVLEHELES
                     LLSSWFDVGMLELRPINWDSPASLLEKLIQYEAVHEILSWEDLRRRVASDRRCYAFFH
                     PRMNGVPLIFVEVAFSQQMADNVQVLLDPDDLAVDPGKMRWAIFYSISNTQSGLRGIS
                     FGNFLLKRVIDELVREFPRLKSFATLSPIPKFADWLGKQDGASIQALLYDKGDKQAAA
                     DDPGAGAKWVERLRAAATDASCADTVRRSGLRLAAHYLKSMKNNQPLDPVARFHLGNG
                     ARIERVNWAGDTSPKGLTQSCGVMTNYVYELDELDDNFARLNAGRPRVSRAIQWL"
     gene            6788..7600
                     /locus_tag="VFR20_06285"
     CDS             6788..7600
                     /locus_tag="VFR20_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU0230159.1"
                     /translation="MSDSRAGEVAEAGRIDVEVDGVIAMVTLNHLGRLNALTVDMWRA
                     LGNVFNELSANAALRCVVLRGAGGNFAAGADIREFPRERADREGVMHYHLRVIAPTLS
                     AIHMCAHPVVAAIEGVCVGGGLEVACQCDVRIAGRSARFGVPINRLGFPMAPDEMRGL
                     LALVGKAATLEILLEGRVFDAIEAQAKGLLTRVVSDETVWDEALRSAQRIARGAPLAA
                     RRNRRLADRLWPAPPPLSDDELRRFFDYADSQDHRHGVEAFLAGTEPTFTGD"
     gene            7603..>8606
                     /locus_tag="VFR20_06290"
     CDS             7603..>8606
                     /locus_tag="VFR20_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0230160.1"
                     /translation="MEANANLYALLANGFPADREAVAIETPARTYSWADIEQTSARLA
                     NFLASLGAPPGSRIAVQVGKSPEAAMLYLAALRTGLIYLPLNTAYRAAEVEYFLTDAE
                     PSVVICDSANLEWVGELAGKTGVAHVHTLDANGEGSLMTAAASQAATFQTVRSQPDDV
                     AAILYTSGTTGRSKGAMLSHRNLSSNALTLKTFWGWRADDVLLHMLPIFHVHGLFVAF
                     HGALLAGARMIWLPKLDIDQALHYLPRCTVMMGVPTYYVRLLADARFTREACRGMRLF
                     ISGSAPLLEETFRQFQERSGLRILERYGMSETGMIASNPYDPALGERLAGTVGPALPG
                     "
BASE COUNT         1526 a         2475 c         2795 g         1810 t
ORIGIN      
        1 tgacgatgcg attgagttcg gtgatttcgg cctcggtcag gtagttcttg gccacgctga
       61 catcggcttt ctgaacctgg cccttgcggg tgctggtgag gcccatgtcg ggccgggtgt
      121 ggtcggcgcg gttgcggatg atctcggccg ccgtctggcc ggacacggcg tagtgcagct
      181 tgttctggat gatgcggaag aaggccgtgg tttccggcag ggtaggggtg tagtcggcgg
      241 ccagggcgaa gatttcgcgc acgcgcagat acatgcggcg ttcgctggcg cggatgtcgc
      301 ggatgcgcgc cagcaactcg ccgaagcggt cgggtagcgc cgagtcaccc actggcggat
      361 tcttcaggcg ctcgtcgtcc agcgtgaagc ccttgagcag gtattccgca aggcgttcgg
      421 tggcccagcg gcgaaactgc gtgccacggg gagacttcac ccggtagccg acagccagaa
      481 tcatgtcgag gttgtagagc cgaaccttcc ggcgcacctg ccgggagcct tcggtttgaa
      541 ctgtcaagta atccttgacg gttgccgccg tctgcagttc gccctcggcg agcacgttct
      601 tgacgtggag gctgatgttc tgcttgctgg tctggaacag ctcggtcatc agggcctggg
      661 aaagccacag cgagtcgtcc acgaaccgac attccacccg ggtgcggccg tcctcggttt
      721 catagagcag gaactcggag cctctggttt gtccagactc aatggctgat tgtgggcgtt
      781 gtgtaacggc cggcttacgg ggcttattca tagcccagct ccttcaggta cacagccatt
      841 tgtgcctcca gcccggccaa ctcggccttg agttgctcgc gctcgcggcg cacggccatc
      901 aggtcgatct cggcctcttc ctcgaaggtg tcgacgtagc gcggaatgtt gaggttgaag
      961 tcgttctcgg cgatctcggc cggcgtggcg aggtaggcgt atttgtccac gctttgccgc
     1021 gcggtggcgg tggacaggat gcgccgcagg tcggcctcgc gcagcatgtt ctggttcttg
     1081 ccggcctcaa agtcgcggct ggcgtcgatg aacagcacct tgtcgtcggc cttgttcttg
     1141 cggaacacca gcacggcggc ggggatgccg gtgccgtaga agagtttttc tggcaggccg
     1201 atcaccacgt cgagcaggtt ttcctcgatc agcttccggc ggatgcgccc ctcggccgcg
     1261 ccacggaaca gcacgccgtg cggcacgacg acggccatgc ggccggtgcc gggcttcatg
     1321 atctcgatca tgtgaaggat gaaggcgtaa tcgcccttgg tgcgtggtgg cacgcctcgg
     1381 cggaagcggc tgaacgggtc ggtgtcggcc ccctcgtgcc cccatttttc taggctgaag
     1441 ggcgggttgg cgaccacgat gtcgaagtgc ttgaggctgg aggtgctgtc gagcagcttg
     1501 gggttgcgga tggtgtcgcc ccattcgatg cggtggttgt cctcgccgtg caggaacatg
     1561 ttcatcttcg ccagggccca ggtgccgcca atggcctcct gcccgtagag cgcgtacttg
     1621 cgcgaaccgg tgttttcgcg gaccaggcgg ccgcacttca tgagcagcga tccggagccg
     1681 caagtggggt cgcagatgtc gtcaccttct tgcggcgcca tcaggcgcgc catgagggag
     1741 gacacctcgg gcggggtgta gaactcgccg gccttcttgc cgctggtgga tgcgaaattc
     1801 ttgatgagga attcgtaggc gttgccgatc acgtcgagtt ggccgacgcg gctggggcgc
     1861 aggtctagtt caggcttggc gaaatcttcc agcaggtggc gcaggatgtc gttcttctgc
     1921 tgctcgtcgc ccagcttgtt ggagttgaag ctgatgtcct ggaacacgtc gcgcagcttg
     1981 gtgatgttgg cttcttcgat ggcgtgcagg gccttgtcga tgcgttcgcc gttaccgggg
     2041 ctgtggcgca gggtatgcag gacatggaag ctggcgctgg cgggcagcac gaaacgctcg
     2101 ttcttgagca tctcttcgat tagctcgggg tggtcgccgt actgtttctt gtaggtgtcg
     2161 tagtgatctt gccagacatc ggacacgtac ttgaggaaca gcatggtcag gacaaagtct
     2221 ttgtagatgc tcgggtccac ggtgccacgg aaggtgtcgc aggcgcccca gacggcggcg
     2281 ttgatgtcgt cctggctgac gggggcggtt tggcgggcgc tgctgttcat gatcttgagt
     2341 tccctggtgt gttcagtggg tggcccgcgc cgcgtgcgcc aggtcttcgg cgagtgcctg
     2401 aagctggtgc tcgcggttgt gcatgagttg atggaaggca tggcgctctt ggctggcgag
     2461 ggaggccagg gcaacgatgc gccgttggtc cgccaacgat ggcaggcaca gcgtgagcga
     2521 ctccagcact gggcgccgga tactgagttg gctgctgcct tcggcggctt gctggagctg
     2581 gcgctgaaag ggaggctggt tgatctgcca tgccaggaag gcgggcagca gtggtgcgcg
     2641 tgccgccact tcaaggtgga agaagtgcgg gctgcacacg gcggggctgg gaggctcgtc
     2701 aatgcagacg gcatagaagc gcgcgccctt ggagacgaac aggacatcgc cggccttgag
     2761 ccagtctggc tgtttacggc cagtcaagga ggttcgggtg acgccacgcc agttgacccc
     2821 gtggtcggga tcaacgtctt tcatttgcag ggccagggtt tcgccgtcgg ccaagtcatc
     2881 gatggagccg cggaaagggt agccagcttt caggctggcc aatgctccaa cagtggaaat
     2941 aaatgcgtca atcgtcatga tgcagaatga tatccgcatt cctaagagat gcaagaattt
     3001 ttgcatcata gtgattgatg cgagcagatt tgctcggtgt tttgaaggcg gaaagaggtg
     3061 tcgtggcttt tgctgtaggc cgcagttatt cgaaattttc gaataactgg atcttcggcc
     3121 aacttgatca agcaagatgt ttaaaccgga acgagatctt cgctatcaca cgaggactgc
     3181 gcctcgggcc gctttgtgct ttccgaccca ctccagcccg ccttggaatc gcgcattacg
     3241 atatgaatgt ctcgatcggt ctcgttaacc gcctcgacga tgttctttac ctgttcgtct
     3301 ccattctcat ggcgagcgta tatgaagcgc atcatcgtat ccgcgaactt gggaatgtgt
     3361 cctgtttttt cccaggacgc aatagcctgc tccgtacgtc cgagcgtacg acccaatccg
     3421 ctttgagaca tcaatagggc taaacgcaga tacctgaact cggcacccgt caggtgtgat
     3481 tttttgcgca ccagcacctt gcagatagcc cgtgtgagcc ctttcacatc ctgaattgat
     3541 acggcgctgc catacggtgt ttcgctggtc ttgtatccat tgaccagcca cacattacgg
     3601 agaccgccat ccgtaaagtg atacatattt aatctccaat caaaaacgct gtaaccacaa
     3661 agcaagagga tgcgctttcg ctttcaagag ctacaacggc ggctgtgggc ctgccggcac
     3721 aaaacctttc gatagtgcat ttcatgtggc cggttttgat gtccggctca ggttctttat
     3781 gaatgatgcc gcgttgcaat acatcaagca ctaccgacat ggtgatatga cgcctgcgca
     3841 tctgctgctt ggcgtgttca gtccaggtga ttgaggcgct gtcgcgtgcc agttcatgaa
     3901 ttcgacgttc gtaaccagcc ttgtttagcg ccatggcaat atactaccta tgattttcat
     3961 aggtagcgat aacttttgtg taacttgtta acgcttgctg ctcattggaa ttgttgcgac
     4021 gcacaatatt cacttcctcg catccagtcc tatctggatt gatggtcgac gggctggagt
     4081 tcattgtgcg gatatggcgc ttctgcgaat cgaatgggat cgccgaaggg ttccgcacaa
     4141 agatcaagat gtctcgcgcc gagcgttcgc tagtgggggg tgcacgccag ccaacgcggt
     4201 cacactttgg tcacacgcct cgaacgaggc aaaagaaaac ggcgatccgt tttgtacaca
     4261 gatcgccgtg gctgcttgat tttatttgta ttctggcgga cagggtgaga ttcgaactca
     4321 cggtacgcct aaacgtacac cggatttcga gtccggcgca ttcgaccact ctgccacctg
     4381 tcctggggga ttcggctggc gcgggccctt gaaggggttg cgtcctgccc gataccgtag
     4441 tcggcaatac cgggcgccgt cagcaaatga agtccagcat tctatcacag gaatttcacc
     4501 tcctttttct ggctctggcc catccgtgtg catccggccc cagggatagt ctggataagt
     4561 cgagccggat tacagccccg atccgcagac gcttgcggcg cagtgatttc agacaacccc
     4621 tagggataac catatcgtcc acccattgta ttcactgccc acatctctat aattattaat
     4681 tacagataat gaattacgac gcaatacagt caagagaggc gcgcagtggc tgctcgtatt
     4741 gtccagaaga tcctttatca ggaagtcgcc gaccggctgc gggcgatgat ccagtcgtcc
     4801 gagctgcagg cgggcgaatg ggtggacgaa acccgcttga ccgaaaccct gggtatttcc
     4861 cgcacaccgc tgcgcgaggc gctgaaggtg ctggtggggg aagggttgtt gcgcatcgag
     4921 ccgcgccggg gatgtttcgt caatgagctg tcgtcccgcg acctggatga cattttcccg
     4981 ctgatggcca tgctggaagg gcgctgcgcc tacgaagcgg cgcgcaaggc cagcgaggcc
     5041 gatctgcggc atcttgaacc tttgcatcga caactgcgaa cgcatgccac gacgggcgat
     5101 gtcgaccaat actacgaggc caacgcccgc atccacgagg ccatccaggc gctggccgac
     5161 aaccgctggc tgtccgacct gatcgacaat ctgcgcaagg tgatcagcct gtcgcggcat
     5221 cgcagcctgg cctacgcggg tcgcatcaac gagtcctgcg cggagcatct ggccattttc
     5281 gcggcgttga aggcgcatga tccggatggc gccgaagccc tgacgcgcca gcatctgatg
     5341 cgccagctcg acgcgctgca cgcgatggcc gagcaggaat caagcacaga tgggcggtcg
     5401 ggtgggcagg ggcaaccccc ggcattgcgt caacgtggag tccgatcatg agctcgtccg
     5461 acaagccaaa agatgcccag gccagtgaca gcttggcggc agatactcaa ggacaacgcg
     5521 ttccggtatt gccggaggca cccacccggg gcaagaaaag gcccggtgac gcctcgggcc
     5581 tgctggcgcg cctgagccgg tggctggagc gcgatcagtt gccgcgcgag tccgagatcg
     5641 gaacgctcta tcgggcaaag ctcaccgacg tcgccgccaa caaactggcc aagcaatggg
     5701 gcgagcgcta tctccaggcc gacgggccgg agcgctgttc gctgctggcc gggcttgtgc
     5761 aggcgggtgt tgggctggag ccgcgtggcg accaggggtt tcgcctgttc cggcggctgt
     5821 acgcccagac agacaatttg cagatgctgg tcgaactgcg cgccgacatg ctgcgttggc
     5881 gcaatcaagt cgcggggttg gctgtgctcg agcacgagct cgagagcctg ctgtcgagct
     5941 ggttcgatgt gggcatgctg gaattgcgcc ccatcaattg ggactctccg gcgtcgcttc
     6001 tggaaaaact catccagtac gaggcggtgc atgaaatcct ttcgtgggag gatttgcgcc
     6061 gtcgggtggc gtcggatcgg cgctgctatg cgtttttcca tccacgcatg aacggtgtgc
     6121 cgctgatctt cgtcgaagtg gcgttttccc agcagatggc cgacaacgtg caggtgttgc
     6181 tcgatcccga cgacctcgcg gtggatccgg gcaagatgcg ctgggcgatt ttctattcta
     6241 tttccaatac ccagtcgggc ctgcgcggca tcagtttcgg caacttcctg ctcaaacgcg
     6301 tcatcgatga gctggtgcgc gagtttcccc gactgaagtc tttcgccacg ttgtcgccga
     6361 ttcccaaatt cgcggactgg ctgggcaagc aggatggcgc gtccatccag gctttgctgt
     6421 acgacaaagg cgacaagcag gccgccgcgg atgaccctgg tgccggcgcc aagtgggtcg
     6481 agcggttgcg cgccgccgcc acggatgcga gctgcgcgga cacggtccgg cgcagcggcc
     6541 tgcgcctggc ggcgcattac ctcaagtcca tgaagaacaa ccagccgctg gatccggtcg
     6601 cgcggtttca tctgggcaat ggcgcgcgca tagaacgagt gaattgggcg ggggacactt
     6661 cgcccaaggg gctcacccag tcctgcggcg tcatgaccaa ctacgtgtat gaactggacg
     6721 agcttgacga caatttcgcc cggttgaatg cgggccgtcc gagagtgtcg agggcgatac
     6781 agtggctatg agcgactccc gggccggcga ggtggcggaa gcgggccgca tcgatgtcga
     6841 ggtggatggc gtcatcgcga tggtgacgct caatcacctg ggccgattga acgcgttgac
     6901 ggtcgatatg tggcgggcgt tgggcaacgt gttcaacgaa ttgtcggcca atgccgcgtt
     6961 gcgctgcgtc gtgctgcgcg gcgcgggggg caacttcgcg gcgggcgcgg acattcgcga
     7021 atttccccgc gaacgggcgg accgggaagg cgtcatgcat taccacctgc gggtcatcgc
     7081 gccgacgctg tcggccatcc atatgtgcgc gcatccggtg gttgccgcga tcgagggcgt
     7141 gtgtgtgggt ggcggcctgg aggtcgcttg tcagtgcgac gtgcgcatcg cgggccgttc
     7201 cgcgcggttc ggtgtgccca tcaaccgcct gggcttcccc atggcgcccg atgaaatgcg
     7261 tggcttgctt gccttggtgg ggaaggcggc gacgctggaa atactgctgg aggggcgggt
     7321 cttcgacgcc atcgaggcac aagccaaggg tttgctgacc cgtgtcgtgt ccgacgaaac
     7381 cgtgtgggac gaggcgcttc gcagcgccca acgcatcgcg cgcggcgcgc ctctcgccgc
     7441 gcgacgcaat cggcggctcg ccgatcgttt gtggcccgcg ccgccgccac tgtccgacga
     7501 cgaactgcga agattcttcg attatgccga cagccaggat catcgccatg gggtcgaggc
     7561 cttcctggct ggcacggaac cgacattcac tggagattga acttggaagc gaacgccaac
     7621 ctgtacgcct tgcttgcgaa cggctttccc gctgatcgcg aggccgtggc catcgagacc
     7681 cccgcccgga cctacagttg ggccgacatc gagcagacca gtgcccgcct ggccaatttc
     7741 ctcgcttcgc tcggtgcgcc gcccgggtcg cgtatcgcag tccaggtcgg caagtcgccc
     7801 gaagccgcga tgctgtacct ggcggcgctg cgcacgggcc tgatctatct gccgttgaac
     7861 accgcctacc gcgcggccga agtcgaatat ttcctgaccg atgctgaacc cagtgtcgtg
     7921 atctgcgata gcgccaacct ggaatgggtt ggtgaactgg ccggcaagac cggtgtcgcg
     7981 catgtccata cgctggacgc caatggcgaa ggcagcctga tgacggccgc ggcatcgcag
     8041 gccgcaacgt tccagaccgt gcgaagccag cccgacgacg tggccgccat tctttacacg
     8101 tcgggcacga ccggccgcag caagggggcg atgctctccc acaggaacct gtcttccaac
     8161 gcactgaccc tcaagacgtt ctggggctgg cgcgccgatg acgtcctgct gcacatgctg
     8221 ccgatcttcc atgtgcacgg attgttcgtc gccttccatg gcgcgctgct ggcgggggcc
     8281 aggatgatct ggctgcccaa actcgatatc gatcaggccc tgcattacct gcctcgttgc
     8341 accgtgatga tgggggtgcc gacgtattac gtgcgtctgc tggccgatgc gcggttcacc
     8401 cgcgaggcct gtcgtggcat gcggcttttc atttcggggt cggcgccgtt gcttgaagag
     8461 accttccgtc aattccagga acgcagcggc ctgcgcatcc tggagcgcta tggcatgagc
     8521 gagaccggca tgatcgcgtc gaacccatac gaccctgcgc tgggcgagcg cctggccgga
     8581 acggtgggtc cggcgttgcc cggcga
//
LOCUS       DASTNK010000080        16157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_981441, whole genome shotgun sequence.
ACCESSION   DASTNK010000080 DASTNK010000000
VERSION     DASTNK010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 16157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16157
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981441"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2359
                     /locus_tag="VFR20_06295"
     CDS             <1..2359
                     /locus_tag="VFR20_06295"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEU0230161.1"
                     /translation="FWPGLPGGLSLKGVVGGDVGSHQADLVATYTPAAQKAQRGAAPH
                     GGKMGRPARPTTKSRHRKVSRDPAAHRSDSSAEGPSTDSSVKPSGGSSLASPVRVELR
                     LAGHWGATHGKVGAPDAWRAEVSRLMVDSGGLGARLMAPVKVSFVPQATGSDPRWQVG
                     RADIQVLLPLKQSFVLHSESARGGVGWWDTRGGIARLAVSRRLIEALRKRFAPAGPHY
                     DNGGVHMEGSGHHVDTVLVLSADWNLAFKGALEGRVKLHRVSGDLYVPGDPGFALGLG
                     ALDLDVAATRRGDAASRISATLDLAAAKLGRVSAQGDAVLHTAGQAAWTLDNAVPRTA
                     KVKADITDLGWFNAFLGDQTELGGSLHADVAAASGPHGTWRTSGTLRGKDIKFLRIDD
                     GVRLFNGTLAAHLDGSRLVLDSLRFPAVLRVAPKEWRTAEWVKTSPDAKNGSLTLTGD
                     WDLFASTGTVSAKLYRYPILQRTDRYAMLTGGLKVDATLPSISITGDVKADAGWFDLD
                     VLGSVPTVDNDVVVIRPGQKRTVSVPSDISLDLTIDLGPRFYLTGYGVNSGLVGSMQI
                     LMNGNKLTGVGELRTRGGSIDVYGQHLQLRRGTVTFQGNIANPVLNIEALRTGLPVQA
                     GVRVSGTARKPKIDLVSYPDVSETEKLSWLLLGRAPDEGGGDVALLFSVGASLVGGGE
                     PFYRKLGLDELGIRTGELGSAGSILPPESVVSSANTNPSQLEQQFAQIGKHLTDGVTL
                     SLEQALASTGTVGRLSYKFSRRLRAELSVGTVNGVALIYHVFFDD"
     gene            2515..3078
                     /gene="dcd"
                     /locus_tag="VFR20_06300"
     CDS             2515..3078
                     /gene="dcd"
                     /locus_tag="VFR20_06300"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEU0230162.1"
                     /translation="MSIKSDRWIRRAGGNGMIEPFEAGQVRTANGGRIVSYGTSSYGY
                     DVRCANEFKIFTNINSTIVDPKAFDEKSFVDFQGDVCIIPPNSFALARTVEYFRIPRN
                     VLTVCLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTPLPAKIYAGEGCAQMLFFE
                     SDEVCETSYKDRGGKYQGQQGVTLPRT"
     gene            3249..5510
                     /locus_tag="VFR20_06305"
     CDS             3249..5510
                     /locus_tag="VFR20_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/lysine/ornithine decarboxylase"
                     /protein_id="HEU0230163.1"
                     /translation="MKFRFPIVIIDEDYRSENASGFGIRALAAALEAEGVEVLGVTSY
                     GDLSSFAQQQSRASAFILSIDDEEFDVDSPEDVAYAIKNLRNFIGELRFRNEEIPIYL
                     YGETRTSQHIPNDILRELHGFIHMFEDTPEFVARHIIREARAYLDGLAPPFFRELVKY
                     ASDGSYSWHCPGHSGGVAFLKSPVGQMFHQFFGENMLRADVCNAVDELGQLLDHTGPV
                     AESELNAARIFHADHCFFVTNGTSTSNKIVWHANVASDDVVVVDRNCHKSILHAITMT
                     GAIPVFLRPTRNHLGIIGPIPLEEFDPENIRRKIEANPFARKIKDKRPRILSLTQSTY
                     DGVIYNVEMIKEKLGSEIDTLHFDEAWLPHASFHEFYADMHAIGAGRPRSHDAMVFAT
                     HSTHKLLAGISQASQIIVQESETRKLDRNVFNEAYLMHTSTSPQYAIIASCDVAAAMM
                     EPPGGTALVEESIREALDFRRAMRKVESEYGKNDWWFRVWGPNRLVSDGIGYREDWFL
                     ESGDEWHGFGDLAEGFNMLDPIKATIVTPGLDISGTFAETGIPAALVSKYLAEHGVVV
                     EKTGLYSFFILFTIGITKGRWNTLLTALQQFKDDFDRNQPLWRILPEFCKDHRRYEKL
                     GLRDLCQRIHQAYREHDVARLTTEMYLSDMIPAMKPSDAYAKMAHRQVERVAIDDLEG
                     RVTGVLLTPYPPGIPLLIPGERFNQRIVHYLQFAREFNADFPGFVTDIHGLASETAPD
                     GSIEYYVDCISEA"
     regulatory      5627..5714
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      5737..5857
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            5905..6999
                     /gene="gcvT"
                     /locus_tag="VFR20_06310"
     CDS             5905..6999
                     /gene="gcvT"
                     /locus_tag="VFR20_06310"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEU0230164.1"
                     /translation="MSSALKHTPLHDIHLESGARMVDFGGWEMPVSYGSQIEEHHAVR
                     QRAGMFDVSHMLNADLAGADATAFLRHLLANDVAKLSQPGKALYSCMLNPEGGVVDDL
                     IVYFFAPDRWRIVVNAGTADKDIAWMRRVAAAGRFDVTLAPRRDVAMVAVQGPVAREK
                     AWAARPAWRAATEGLTPFTAAWVDDDTLVGRTGYTGEDGFEIMLPATAVEAFWRDLAA
                     RDVRPCGLGARDTLRLEAGMNLYGQDMDELTQPGESGLSWTVSLKDAERRFIGRAAME
                     SCPGDQVLLGLKLRERGVMRPHMAVRTPLGVGEVTSGTMSPTLGVSIAFARLPAGSKA
                     GDSCEVDIRGKWVPAEMVKPPFVRQGKAVA"
     gene            7089..7466
                     /gene="gcvH"
                     /locus_tag="VFR20_06315"
     CDS             7089..7466
                     /gene="gcvH"
                     /locus_tag="VFR20_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEU0230165.1"
                     /translation="MNLPTDRKYTTSHEWVKTEGDVLLVGITDDAQDQLGDLVFVGDV
                     NPGSKLSAGDTAGVVESVKAASDIYAPVDGEVVAFNEQLESQPELVNQSAFDTWIFKI
                     RPNNRADVDGLLDAAGYEAQSDH"
     gene            7574..10474
                     /gene="gcvP"
                     /locus_tag="VFR20_06320"
     CDS             7574..10474
                     /gene="gcvP"
                     /locus_tag="VFR20_06320"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEU0230166.1"
                     /translation="MLRDLDPHTDFIPRHIGPSSDDQAAMLREIGVDSLQGLVDEVVP
                     RGILMSGKLDLPAARSEPEALAELRDMASRNLVFRSYIGQGYYGTHVPNVILRNILEN
                     PAWYTAYTPYQPEISQGRLEALLNFQTMVADLTGLDIANSSLLDEATAAAEAMTLARR
                     GSRAKSSVFFASHHCHPQTLEVLRTRSTGLGIDVVVGDEADGLPECFGVLLQYPHSLG
                     GVADYRKLVEAAHGKGAVVAVATDLLALALLVPPGEWGADIAVGSAQRFGVPMGFGGP
                     HAGFMACRDVFKRNLPGRLVGVSKDSQGGYALRLALQTREQHIRREKATSNICTAQVL
                     LAVMAGMYAVWHGPQGIRRIARRVASLAALLADTLGALGVPSANTAFFDTVLLETGEH
                     TRAVLDAAHAASINLRRVGPDRVSVSLDETTTSTDLIRLVEVFADALKAPFDASALAA
                     TKVPEPRIPADSLRLTPILAHPVFSRIQSETDMLRYLRSLADKDLALDRSMIPLGSCT
                     MKLNATAEMIPITWPEFANLHPFAPAAQSRGYAELVEGLSTALCKITGYDSVSLQPNS
                     GAQGEFAGLLAIRGYHQANGQAQRDVCLIPASAHGTNPASAQMAGMKVVVVASDDNGS
                     IDVADLKEKIADVGDHLAALMLTYPSTHGVFESAVTEICDLVHQAGGQVYLDGANMNA
                     MVGLAQPGKFGSDVSHLNLHKTFCIPHGGGGPGIGPVAVRAHLAPYLPGVVDESGRLP
                     DAAHSGPVSAAPYGSAGILPIPYLYILMMGADGLKQASQVAILNANYIAHRLRKHYPI
                     LYASPKGRVAHECILDIRPLKDGCGISAEDIAKRLIDYGFHAPTMSFPVPGTLMVEPT
                     ESEGLVELDRFVDAMIAIRAEIAQVERGEKDAEDNVLRNAPHTASMLLATDWHHDYAR
                     DEAAYPVASLRDGKYWPPVARVDNAYGDRHLVCSCPPMEDYMDADAAERSPA"
     gene            complement(10584..11165)
                     /locus_tag="VFR20_06325"
     CDS             complement(10584..11165)
                     /locus_tag="VFR20_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase"
                     /protein_id="HEU0230167.1"
                     /translation="MALEELLHAVGKLTRLVERTELREPEILEQAGGIIRDLVAKDDW
                     LPPDMAQSDPNRYRQYLLYGDPLDRFSLVSFVWGPGQHTPIHDHTVWGVIGMLRGAEV
                     SQSYRANADGTLAADGPEEVLRPGRIACVSPSIGDIHRVSNAHQDKTSISIHLYGGNI
                     GRINRSVYDAATGARTSFVSGYSNASLPNLWKQ"
     gene            complement(11192..12517)
                     /locus_tag="VFR20_06330"
     CDS             complement(11192..12517)
                     /locus_tag="VFR20_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU0230168.1"
                     /translation="MSSPTAIPNLSHLWMPFTANRQFKAHPRLLTGAKGMYYTTSDGK
                     QILDGTSGLWCVNAGHGRQEIVDAISRQAAELDYAPSFQIGHPNSFLAATAIAELMPE
                     GLDRIFFTNSGSESVDTALKIALAYHRARGEGQRTRLIGRERGYHGVGFGGISVGGIS
                     PNRKAFSSSLIPGVDHLPHTHDLSKNANSRGQPAWGEHLADELERLVALHDASTIAAV
                     IVEPLAGSTGVLIPPKGYLEKLRTITSKYGILLIFDEVITGYGRLGAPTASEFFGVTP
                     DLITMAKGVSNAAVPAGAVAVRREVHDTIVNATAAGIEFFHGYTYSGHPLATAAVLAT
                     LDIYAKEKLFDRARELSVKFEDAAHQLESARHVVDVRNLGLVAGIELESRPGAVGARV
                     AEAFAKCMERGVMVRYTGETIALSPPLIIEEAQIDQIFSTLADVLKTVD"
     gene            12765..14186
                     /locus_tag="VFR20_06335"
     CDS             12765..14186
                     /locus_tag="VFR20_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU0230169.1"
                     /translation="MILFEPIRDRIHAPTLVEQVVKALEDAISSQLLRPGMAVPSVRR
                     FARDHGLSNFTVATAYSRLVAQSWLSARPGSGYRVAMRTARPHEAAPARWRPPEIGAS
                     WLLADVFADHSIPIKSGCGWMPPEWLNETGLQQSLRQLARVPAEQIAGYGHPFGYAPL
                     REHVAGSLGEYGLAADADNVLLTLGASQALDIVLRAVFRHGDEVAVETPCYANFLQTL
                     TLSGLSAVGIPRTEEGLCVDTLERVASRRKLKAVFVNTVLHNPTGSSLTMANAFRILR
                     IAEQYNLRVIEDDVSRGLLPGVGPMLAALAGPERVIYVSGFSKSVAPSVRVGYVVAAP
                     ALLDGFAKIKMAMGLTTPEMMERAVYQLLRLGHHHAHLRRVQDRLREAHDALCDLLER
                     HGFEVFCRPRAGLFLWARPGGEWRERGAARLAELALKDGIWLAPGSYFHPEQLDEGWI
                     RFNVAYSLDASLWRFMRKVGATV"
     gene            complement(14190..14678)
                     /locus_tag="VFR20_06340"
     CDS             complement(14190..14678)
                     /locus_tag="VFR20_06340"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU0230170.1"
                     /translation="MTRPTIKLVVAYSDNRAIGRDNALPWRLKADLAHFKNTTMGCPI
                     LMGRNTWDSLGRPLPGRRNLVISRNPGLRADGASAYPSLRDALNDCQDEATVFVIGGA
                     QIYAQVLPMADEVIATEVHAHVDGDAFFPALPPDQWEEVERQRQPEESGLAFDFVVYR
                     RR"
     gene            complement(14698..15492)
                     /locus_tag="VFR20_06345"
     CDS             complement(14698..15492)
                     /locus_tag="VFR20_06345"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU0230171.1"
                     /translation="MQQYEDFMRHVYEHGQTKTDRTGTGTRSVFGHQMRFDLNEGFPL
                     ITTKKLHTKSIFIELLWFLRGDSNVRWLQERGVSIWDEWADADGNLGPVYGAQWRSWP
                     TRNGGHIDQISQIVRQIRETPDSRRLIVSAWNVGDIPDMALPPCHALFQFYVANGKLS
                     CQLYQRSADIFLGVPFNIASYALLTHLVAQQCDLEVGDFVWTGGDCHLYSNHFEQARL
                     QLSRTPFPYPRLNIRRRPPSLFEYEYDDFKILDYQAHPHIKAPVAV"
     gene            complement(15588..16010)
                     /locus_tag="VFR20_06350"
     CDS             complement(15588..16010)
                     /locus_tag="VFR20_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU0230172.1"
                     /translation="MECTIDWGGPDGMLFVARTGSGHIATMDGAPDGGGHNLAPRPME
                     MVLCGTGACTAYDVVLILQRGRHAVTDCRVTLTSERADTDPKVFTKIHFAFTVTGSRL
                     TRDAVERAVRLSHEKYCSASAMLAKTAEITWSAEIVEA"
BASE COUNT         2765 a         4999 c         5345 g         3048 t
ORIGIN      
        1 gttctggcca ggcctgccgg gcggattgag cctcaaggga gtggttggag gggatgtggg
       61 cagtcatcaa gccgatctgg tcgccacgta cacgcccgct gcgcaaaagg cgcaacgtgg
      121 cgccgcgccg catggtggca agatgggacg gccggctcgc ccgacgacaa aatcccgtca
      181 tcggaaagtc tcccgcgatc cagccgccca ccgctccgac agttccgcag agggcccctc
      241 caccgactca tccgtcaagc cgtcgggcgg ttcgagtctg gcttctcccg tgcgcgtcga
      301 gcttcgtctc gccgggcatt ggggtgccac gcatggcaag gtgggggcgc cggacgcttg
      361 gcgggccgag gtgtcccggt tgatggtgga ctccggtggc ctgggggcgc ggctgatggc
      421 gccagtcaag gtgtcgttcg tgccacaggc cacgggcagc gacccccggt ggcaagtcgg
      481 cagggccgac atccaggtgc tgttgccgct caagcagtcg ttcgtgcttc acagtgagtc
      541 ggcgcgcggc ggtgtgggtt ggtgggacac acgcggcggg atcgcccggc tggccgtgtc
      601 gcggcgcctg atcgaggcgc tgcgcaagcg ctttgcgccg gcggggccgc attatgacaa
      661 cggtggcgtc catatggagg gcagcggcca ccatgtggac acggtcttgg tgctgtccgc
      721 ggactggaac ctggcgttca agggcgccct cgaagggcgg gtcaagctgc atcgggtgtc
      781 gggtgatcta tacgtgccgg gtgatccggg attcgcgctg gggttgggcg cgctggatct
      841 ggacgtggcg gccacacggc gcggcgatgc cgccagccgg atctcggcca cgctggatct
      901 ggccgcggcc aagctgggcc gcgtgtcggc gcaaggcgat gccgtcttgc acacagccgg
      961 ccaagccgcc tggacgctgg ataatgcggt gccccggacg gcgaaggtca aggccgacat
     1021 caccgatctg ggctggttca acgcttttct gggtgatcag accgaactgg gcggttcgct
     1081 gcacgccgat gtcgccgccg ccagcggtcc gcacgggact tggcgcacca gcggcacgct
     1141 gcgcggcaaa gacatcaagt ttcttcgcat cgatgacggc gtgcgcctgt tcaacgggac
     1201 gctggccgcg catctggatg gcagccggct ggtgctggac agtctgcgct tcccggcggt
     1261 gttgcgcgtc gcgcccaagg agtggcggac ggccgagtgg gtcaagacca gtccggacgc
     1321 gaagaacggt agcctgaccc tcaccggcga ttgggacttg ttcgcgtcca ccggcacggt
     1381 cagcgccaaa ctctaccgtt atcccatcct gcaacgcact gatcgctatg ccatgctgac
     1441 cggcggattg aaggtcgatg ccacgctgcc atcgatctcg atcaccggcg acgtcaaggc
     1501 ggatgcgggc tggttcgatc tggacgtcct gggcagtgtg ccgaccgtcg acaacgacgt
     1561 ggtggtgatc cggccgggtc agaagcggac cgtgagcgtc ccgtcggata tctcgctgga
     1621 cctgaccatc gacctgggac cgcgtttcta tctgacgggg tatggggtga actccgggct
     1681 ggtggggagc atgcagatcc tgatgaacgg caacaagctc acgggtgtgg gagaactgcg
     1741 cacgcgcggc ggctccatcg acgtttacgg gcagcacttg caactgcgcc ggggcaccgt
     1801 gacgttccag gggaatatcg ccaatccggt gttgaacatc gaagccttgc gcaccggtct
     1861 gccggtgcag gccggcgtgc gggtgagcgg gacggccagg aaacccaaga tcgacctggt
     1921 gtcctacccc gatgtcagcg aaaccgagaa actgtcctgg ctgttgctgg gccgtgcgcc
     1981 cgacgagggc ggtggcgacg tggcgctgtt gttttccgtg ggcgcgtccc tcgtgggtgg
     2041 tggcgagccg ttctatcgca agctggggct cgacgaactg ggaatccgca ccggcgagct
     2101 gggcagcgcc ggcagcattc tgccgccgga aagcgtggtc agcagcgcca atacgaaccc
     2161 cagccagctc gaacaacagt tcgcccagat cggcaaacac ctgaccgacg gggtgacgct
     2221 cagcctggag caggcgctgg cctccaccgg cacggtgggg cggctcagtt acaagttttc
     2281 ccgcaggctg cgcgcggaac tgagcgtcgg caccgtcaat ggcgtcgcct tgatctacca
     2341 tgtgtttttt gacgattgaa gccccggatg catgcggcgc ggtgacttgt caaaagggtc
     2401 cccgggtgtt gtttttcgtc gtctgcatcc ggtttttcat cgtcccggcg cctcggcgct
     2461 gcaccgcgtg gcgctgcgcg ataaaatgag cgcccattaa acagggtact ggacatgagc
     2521 atcaagagcg atcgctggat caggcgcgcg ggcggcaatg ggatgataga gcccttcgag
     2581 gccgggcagg tccgcacggc caatggcggg cgcatcgtca gctacggcac cagcagctat
     2641 ggctacgatg tgcgttgcgc caacgaattc aagatcttca cgaacatcaa ttcgaccatc
     2701 gtcgacccca aggccttcga cgagaaatcg ttcgtcgatt tccagggcga tgtctgcatc
     2761 attccgccga attcctttgc cctggcccgc actgtggagt acttccgcat ccctcgcaat
     2821 gtcctgacgg tctgcctggg caagagcacg tatgcgcgtt gcggcatcat cgtgaacgtg
     2881 accccgctcg aacccgaatg ggaaggccac gtcacactgg aattttcgaa caccacgccg
     2941 ctccccgcca agatatacgc tggcgagggc tgtgcgcaga tgctgttttt tgaaagcgat
     3001 gaagtgtgcg aaacctcgta caaggatcgc ggcggcaaat accaggggca acagggcgtg
     3061 accctgcctc gaacttagcg tacggttccg tgggtgcacg gggactgccc actgtcgtcc
     3121 tgcccggctg gctgatgagc caccaggcag gactccagcg tcattttgta aaacgtttcg
     3181 gggctcgtga cgaacccgcc ggcacgattt gggtgtcggc gtgaacggac cccaaccgga
     3241 gccatgccat gaaattccgc ttccccatcg tcatcattga cgaagactac cgctccgaga
     3301 acgcctcggg cttcggcatc cgggccctgg cggcggcgct cgaggccgag ggcgtcgaag
     3361 tgctgggtgt gaccagctac ggcgacctca gttcgttcgc gcagcagcaa agccgggcga
     3421 gcgcgttcat cctgtcgatc gacgacgagg aattcgacgt cgattcgccg gaggacgtgg
     3481 cctatgccat caagaacctg cgcaatttca ttggcgagct ccggttccgc aatgaagaaa
     3541 tccccatcta cctgtatggc gagacgcgca cgtcgcagca cattccgaac gacatcctgc
     3601 gcgaactgca tggcttcatc cacatgttcg aggacacgcc ggaattcgtg gcgcgccaca
     3661 tcatccgcga ggcccgggcc tacctcgacg gcctggcgcc gccgttcttc cgcgaactgg
     3721 tcaagtacgc cagcgatggt tcctattcct ggcactgtcc ggggcattct ggcggggtgg
     3781 ccttcctgaa aagcccggtt gggcagatgt tccaccagtt ctttggcgag aacatgttgc
     3841 gcgccgacgt gtgcaacgcg gtcgacgaac tggggcagtt gctggaccac accgggccgg
     3901 tggcggaatc ggagctgaac gcggcgcgca tattccatgc cgaccattgc ttcttcgtga
     3961 ccaacggcac gtccacgtcg aacaagatcg tctggcacgc caacgtggcc tctgacgacg
     4021 tggtggtggt cgaccgcaat tgccacaaat ccattctgca cgccatcacc atgacgggcg
     4081 cgatacccgt gttcctgcgg cccacgcgca accatcttgg catcatcggg cccattcccc
     4141 tggaagagtt cgacccggag aacatccgcc gcaagatcga ggccaatccg ttcgcgcgca
     4201 agatcaagga caaacggcca cgcatcctgt cgctgacgca aagcacgtac gacggcgtca
     4261 tctacaacgt cgagatgatc aaggagaagc tcggatccga gatcgacacc ctgcatttcg
     4321 acgaagcctg gctgccacat gcctcgttcc atgagttcta tgccgacatg cacgccattg
     4381 gcgcgggtcg tccgcgcagc cacgatgcca tggtgttcgc cacgcactcc acgcacaaat
     4441 tgctggccgg catttcgcag gcctcgcaga tcatcgtgca ggagtccgag acccgcaagc
     4501 tcgaccgcaa cgtgttcaat gaggcgtatc tgatgcacac gtccacatcg ccgcaatacg
     4561 ccatcatcgc gtcctgcgac gtggcggcgg ccatgatgga accccctggg ggcacggcgc
     4621 tggtcgagga aagcattcgc gaggcactgg atttccggcg cgccatgcgc aaggtggagt
     4681 ccgagtatgg caagaacgac tggtggtttc gcgtgtgggg cccgaaccgc ctggtcagcg
     4741 acggcatagg ctaccgtgaa gactggttcc tggagtcggg cgacgaatgg cacggcttcg
     4801 gcgacctggc cgaggggttc aacatgctgg acccgatcaa ggccaccatc gtcacccctg
     4861 ggctggacat ctcgggcacg ttcgccgaga ctggtattcc cgccgcgctc gtttccaaat
     4921 acctggccga gcatggcgtg gtggtcgaga agaccgggtt gtattcgttc ttcatcctgt
     4981 tcaccatcgg catcacgaaa ggccgctgga acacgctgct gaccgccctg cagcagttca
     5041 aggatgattt cgaccgcaac cagccgctgt ggcgcattct gcccgagttc tgcaaagatc
     5101 atcgccgcta cgaaaagctg gggctgcgcg acctgtgcca gcgcatccac caggcctatc
     5161 gcgagcacga cgtggcgcgc ctgaccaccg agatgtacct gagcgacatg attccggcga
     5221 tgaagccatc ggacgcctac gccaagatgg cgcaccgcca ggtcgagcgc gtggccatcg
     5281 acgatctcga aggccgggtc accggcgtgc tgctcacgcc atatccaccg ggcatcccct
     5341 tgctcatccc cggcgagcgg ttcaaccagc gcatcgttca ttacctgcag ttcgcgcgcg
     5401 agttcaacgc cgatttcccg ggcttcgtca ccgacattca tgggttggcg agcgagaccg
     5461 cgccggacgg ctcgatcgaa tactacgtgg actgcatcag cgaggcctga cccgcagcct
     5521 ggcctggccg tgccggtgcg ccacggtcgg gcattccagt caggcgggaa aagacaattt
     5581 tcttggtgtt tgccgccatt tcgcggtatg ctgtgcgctg ctgtcaatac atagagtggg
     5641 agagtgccgg aaccccggcc gccgaaggcg caattcgccc agaatcgctc aggcccccgt
     5701 accgctctcg tgtgccgtcg cgcttgttgc gcgatggggc ggctctctgg agagaccccc
     5761 gcgccgccac catggccgcg acggcgggcg ccgaaggtga atccttgccg cgttatcggc
     5821 caaggcaact ctcaggcaaa aggacagaag ggcgacgttg ccactacctg gttccctcgt
     5881 tgcctgtccc cggagtttca ttcaatgtct tcagccctga agcatacccc tctgcatgat
     5941 atccacctcg aatccggtgc ccggatggtt gatttcggcg gctgggaaat gcccgtgtcg
     6001 tacgggtcgc aaatcgaaga gcatcacgcg gtgcgccaac gcgccggcat gttcgatgtg
     6061 tcccatatgc tcaatgccga cctggccgga gccgatgcca ccgcgttcct gcggcacctg
     6121 ttggccaacg acgtggccaa gctgtcgcaa cccggcaagg cgctgtattc ctgcatgctc
     6181 aacccggaag gcggggtggt cgacgatctg atcgtctatt ttttcgcgcc cgaccgctgg
     6241 cgcatcgtgg tcaatgcggg cacggccgac aaggatatcg cctggatgcg ccgggtggcc
     6301 gcagccggcc ggttcgacgt caccctcgcg ccgcgccgcg atgtggccat ggtggcggta
     6361 cagggcccgg tcgctcgcga aaaggcctgg gcggcgcgcc ccgcgtggcg ggccgccacc
     6421 gaagggttga cccccttcac cgcggcgtgg gtcgacgatg acacgctggt gggccgcacc
     6481 gggtacacag gcgaagacgg gttcgagatc atgttgcccg cgaccgctgt cgaagctttc
     6541 tggcgggatc tcgcggcacg ggacgtccgc ccctgcgggc tgggtgcgcg cgatacgctg
     6601 cgcctcgaag ccggcatgaa tctgtacggc caggacatgg atgaactgac gcagccgggg
     6661 gaatccgggc tgtcctggac ggtgtcgctc aaagatgcgg agcgccgctt cattggccgc
     6721 gccgccatgg agtcctgtcc tggcgaccag gtgttgctcg ggctgaaatt gcgggagcgt
     6781 ggtgtgatgc gcccgcacat ggcggttcgt accccgctgg gagtgggcga ggtcaccagc
     6841 ggcaccatgt cgccgacgct gggtgtgtcc attgcgtttg cccgcctgcc ggccggcagc
     6901 aaggctggcg attcctgcga ggtcgacatt cgcggcaagt gggtgcccgc cgaaatggtc
     6961 aagccgcctt tcgtgcgcca gggcaaggcg gtggcctgac cggccacgcg agcttcggtt
     7021 ttcattgcgt ttcaacccat tccggatctt cgttccggga tccacatttt taacaggatc
     7081 gactattcat gaacttgcca accgatcgca aatacacgac ctcgcacgag tgggtcaaga
     7141 ccgagggtga cgttctgctg gtgggaatca ccgatgacgc gcaggaccaa ctgggcgacc
     7201 tggtcttcgt gggcgacgtc aaccctggct caaagctgtc cgccggcgac accgccgggg
     7261 tggtcgaatc ggtcaaggcc gcgtccgaca tctacgcgcc ggtcgacggc gaggtcgtcg
     7321 cgttcaacga gcagctcgaa tcgcagcccg aactggtgaa ccagtcggcc ttcgacacct
     7381 ggatcttcaa gatccgtccc aacaaccggg cggacgtcga cgggttgctg gacgcggcgg
     7441 gctacgaggc ccagagcgac cactgagttc ttgtccttga aacatcaagg ccggccgccc
     7501 ggatggcgct ggcctgaccg cacggtttcc gcctttttct tcacacggtt ttcgcacttt
     7561 tctataagcc accatgttgc gcgatctgga tccccatact gatttcatcc cccgccatat
     7621 cgggccctcg tctgatgatc aggcggccat gctgcgcgaa atcggcgtcg acagccttca
     7681 gggcctggtc gacgaggttg tgccgcgcgg cattctcatg tccgggaagc tcgacctgcc
     7741 cgccgcgcgc agcgagccgg aggcgttggc cgaattgcgc gacatggcgt cgcgcaacct
     7801 ggtgttccgc agctatatcg gccagggcta ctacggcacg cacgtgccca acgtgatcct
     7861 gcgcaacatt ctcgaaaatc cggcctggta caccgcgtac acgccgtacc agcccgagat
     7921 ctcacagggc cgcctcgaag ccctgctgaa tttccagacc atggtcgccg acctgaccgg
     7981 gttggacatc gccaattcgt cgttgctgga tgaagccacc gccgccgccg aggccatgac
     8041 cctggcgcgt cgcggttcgc gcgccaagtc cagcgtgttc ttcgcgtcgc accactgcca
     8101 tccccagacg ctggaggtcc tgcgcacccg ctcgacgggg ctgggcatcg atgtggttgt
     8161 cggcgatgag gccgatggct tgccggaatg cttcggcgtg ttgttgcagt atccgcacag
     8221 cctgggcggg gtggccgatt accggaagct cgtcgaggcc gcgcacggaa aaggtgcggt
     8281 ggtggcggtc gccaccgacc tgctggcgct ggccctgctg gttccgccgg gcgaatgggg
     8341 cgccgacatc gctgtgggtt cggcccagcg gtttggcgtt cccatgggat tcggcggccc
     8401 gcacgccggg ttcatggcct gccgcgatgt gttcaagcgc aacctgccgg gtcgcctggt
     8461 gggggtgtcc aaggacagcc aggggggcta tgcgctgcgc ctggccttgc agacgcgcga
     8521 gcagcatatc cgccgagaaa aggccacatc caacatttgc acggcccagg tgctgctggc
     8581 tgtcatggcg ggcatgtacg ccgtgtggca tggtccccag ggcatccggc gcatcgcccg
     8641 gcgcgtggca tcgctcgcgg ccctgttggc cgacacgctg ggcgcactgg gcgtgccgtc
     8701 cgccaatacc gctttcttcg acacggtgct gcttgaaacc ggtgaacata cccgcgccgt
     8761 actcgatgcc gcccatgcgg cgtccatcaa cctgcgccgg gttggcccgg atcgcgtatc
     8821 ggtgtcgctg gatgaaacca ccacgtcgac cgatctgatc cgtttggtcg aggtgttcgc
     8881 cgacgcgctc aaggcgccgt tcgatgcgtc cgcgctggcc gccaccaaag tgcctgaacc
     8941 ccgtattccg gcggattcgc tgcgcctgac tcctatcctg gcgcacccgg tgttctcgcg
     9001 catccagtct gaaaccgaca tgctgcgcta tctgcgtagc ctggccgaca aagacctggc
     9061 gctggatcgt tccatgatcc ccctggggtc gtgcaccatg aagctcaacg ccacagccga
     9121 gatgatcccc atcacctggc ccgagttcgc caacctgcac ccgttcgccc cggcggcgca
     9181 aagccggggc tatgcggaac tggtcgaggg gctgtccacg gcgttgtgca aaatcacggg
     9241 ctacgattcc gtcagcctgc agcccaattc cggggcgcag ggcgaattcg ccggattgct
     9301 ggccatccgc ggctaccatc aggcaaacgg acaggcgcag cgcgatgtct gcctgatccc
     9361 cgcctcggcg catggcacca accccgcctc ggcgcagatg gccggcatga aggtggtcgt
     9421 ggtggcctcg gacgacaacg gcagcatcga cgtcgccgac ctgaaagaga agatcgccga
     9481 cgttggcgac catctcgcgg cgctgatgct gacctacccc tccacgcacg gcgtgttcga
     9541 atcggcggtg accgagatct gcgacctcgt gcaccaggcc ggcggccagg tgtatctgga
     9601 cggcgccaac atgaacgcca tggtggggtt ggcgcagccg gggaaattcg gctccgacgt
     9661 ctcgcatctg aacctgcaca agacgttctg catcccgcat ggcggtggcg gcccgggcat
     9721 cggcccggtc gcggtgcgcg cgcacctggc gccgtacctg cccggtgtgg tggacgagtc
     9781 cggccggctg cccgatgcgg cgcattcggg cccggtgtcg gccgcgccgt atgggtcggc
     9841 cggcattctg cccattccgt acctgtacat cctcatgatg ggggcggacg ggctgaagca
     9901 agcctcgcaa gtggcgattc tcaacgccaa ttacatcgcg catcgcctgc gcaagcacta
     9961 ccccatcctg tatgcgagcc cgaaggggcg cgtggcgcat gagtgcattc tcgacatccg
    10021 cccgctcaag gacggttgcg ggatctcggc cgaggacatc gccaagcgcc tcatcgacta
    10081 cggtttccat gcgccgacca tgagcttccc ggtgcccggc acgctgatgg tcgagcccac
    10141 cgagtccgaa gggctggtcg agctcgatcg tttcgtcgac gccatgatcg ccatccgcgc
    10201 cgagatcgcg caggtggaac gtggtgaaaa ggacgccgag gacaacgtgc tgcgcaatgc
    10261 gccgcatact gcgtccatgc tgctcgcgac cgactggcac cacgattacg cgcgcgacga
    10321 ggccgcgtat cccgtggcca gcctacggga cggcaagtac tggccgcccg ttgcgcgcgt
    10381 ggacaacgcc tatggcgatc gacatctggt ctgctcgtgc ccgcccatgg aggattacat
    10441 ggatgcggac gcggctgaaa ggtcgccggc ttgatcatgt gactgtcgag caaagaaaaa
    10501 cccgcttacg cgggtttttc ttcatgtggc agactttgtc tcaagcccct ttttgcctca
    10561 gactcccggc ccgggcgctg agcctattgt ttccacaggt tcggcaacga ggcatttgaa
    10621 tagcccgaaa cgaaagacgt gcgggcgccg gtcgcggcat cgtacacgct gcggttgatt
    10681 cgaccgatgt ttccaccgta caaatgaatg ctgatggagg tcttgtcctg gtgggcgttg
    10741 ctgacgcggt gaatgtctcc aatgctgggg gacacgcacg cgatccgtcc gggccgcaac
    10801 acctcttcgg gcccatccgc cgccagggtt ccgtcggcat tcgcgcgata cgactgtgac
    10861 acttcagcgc cgcgcaacat gccgatgacc ccccatacgg tgtggtcatg tatgggtgtg
    10921 tgctggcccg gaccccacac aaaactcacc aatgaaaacc ggtccagggg atcgccatac
    10981 aacagatatt gacgatagcg gttgggatcg gattgcgcca tatcgggcgg cagccagtcg
    11041 tctttggcga ccaggtcgcg gatgatcccc ccggcctgtt caagaatctc gggttcccgg
    11101 agctccgtcc gctccaccaa ccgcgtgagt ttccccacgg cgtgcaagag ttcttcgagc
    11161 gccaagtgat ttcctcctgc gggccgcgga atcagtccac cgtcttcagc acatcggcca
    11221 gcgtgctgaa gatttggtcg atctgtgctt cctcgatgat gagcgggggt gacagggcga
    11281 tggtttcgcc ggtgtagcgg accatgacgc cccgttccat gcatttggcg aatgcctcgg
    11341 ccacgcgggc acccaccgcg cccggtcggg actccagttc gatacccgcg accaggccca
    11401 ggttgcggac gtcgacgacg tggcgcgcgc tttccaactg gtgggcggcg tcttcgaact
    11461 tgaccgacaa ttcgcgcgcc cggtcgaaca gtttttcctt ggcgtagatg tcgagtgtgg
    11521 ccagcacggc cgccgtggcc aacgggtggc ctgaataggt gtagccgtgg aagaactcga
    11581 tgccggcggc cgtggcgttg acgatggtgt cgtgcacctc gcgacgcacc gcgaccgcgc
    11641 cggcgggaac ggccgcgttg ctgacgccct tggccatggt gatcaggtcg ggggtgacgc
    11701 cgaagaattc gctggcggtg ggcgcgccca ggcgcccgta cccggtgatc acctcgtcga
    11761 aaatcagcag gatgccgtac ttgctggtga tggtgcgcag tttctccaag tagcctttgg
    11821 gcgggatgag cacgccggtg gaacccgcca gcggctcgac gatgacggcg gcgatggttg
    11881 acgcatcgtg cagggcgacc aggcgttcga gttcgtcggc cagatgctcg ccccaggcgg
    11941 gttggccccg gctattggcg tttttcgaca ggtcgtgcgt gtgcggcagg tgatcgacac
    12001 ccgggatcag cgacgacgag aacgctttgc ggttgggcga aataccgccc accgagatac
    12061 cgccgaaacc cacgccgtgg tagccgcgtt cgcggccaat cagtctggtg cgctggccct
    12121 cgccgcgcgc gcgatggtag gcgagggcga tcttcagcgc cgtatcgacg gactcggaac
    12181 cagagttggt gaagaaaatg cgatccaggc cttcaggcat cagctcggcg atcgcggtgg
    12241 cagccaggaa cgaattgggg tggccgatct ggaagctggg cgcgtagtcg agttcggccg
    12301 cctggcgtga aatggcatcc acgatttcct ggcgcccgtg ccccgcgttg acgcaccata
    12361 ggccggaggt gccgtccaga atctgcttgc catcggacgt ggtgtagtac atgcctttgg
    12421 ccccggtcag gagcctgggg tgcgccttga actgccgatt ggcggtgaat ggcatccaca
    12481 ggtgcgacag gttgggaatg gcggtagggc tgctcatgat gggggatctc gaagatggat
    12541 gaacgggtcg tcgcctcgac ggacgcgtgg cgctgcggag gctatgagct attctcgatc
    12601 cggccatcct ggaaggaaat atacagtcta ttaataattc tcaaactgta ttgtcaaaat
    12661 cttaataaac tgtacataat taagtgccga tgacgatgcc gatgccgatg taataaaaga
    12721 gcgccgggcc gcgccgcatg cgtctgcccg ccatggaggc ttgaatgatt cttttcgaac
    12781 ccatcagaga tcggatccat gcgcccacgc tggtggaaca ggtggtgaaa gcgctggagg
    12841 acgcgatttc gtcgcaattg ctgcgtccgg gcatggctgt tccgtcggtg cgacgattcg
    12901 cgcgtgatca tggcctcagc aatttcaccg tggccacggc ctatagccgg ttggtggcgc
    12961 aaagctggct gtccgcgcgc cccggttccg gataccgcgt cgccatgcgc accgcacgcc
    13021 cgcatgaggc ggcgccggct cgctggcgtc cgcccgagat tggcgcgtcg tggctgctgg
    13081 ccgatgtctt cgccgatcat tccattccaa tcaagtcagg ctgcggctgg atgccgcccg
    13141 aatggctcaa tgaaacgggg ttgcagcagt cgttgcgcca gctggcgcgc gtacccgccg
    13201 agcagatcgc gggttatggc cacccgttcg gctatgcacc gctgcgtgag cacgtcgcgg
    13261 gcagcctggg ggaatatggc ctggcggccg atgcggacaa tgtgctgctg acgctcgggg
    13321 cttcccaggc gctggacatc gtgctgcgcg ccgtgtttcg gcacggtgac gaggtggcgg
    13381 tcgaaacgcc ttgctatgcc aatttccttc agacgttgac gctatcgggg ctgtcggcgg
    13441 tgggtatccc acgcacggaa gaaggactgt gcgttgatac gctcgaacgc gtcgcgtcgc
    13501 gtcgcaagct caaggcggtg ttcgtgaaca ccgttttgca caaccccacg ggttcaagcc
    13561 tgacgatggc caacgcgttc cgcattttgc ggatcgccga acaatacaat ctgcgggtca
    13621 tcgaggacga cgtgtcgcgc gggttgttgc ctggcgtcgg cccgatgctg gccgctctgg
    13681 ccggaccgga gcgcgtgatt tatgtatcag ggttttccaa gagtgtggcg ccatctgtgc
    13741 gcgtgggtta tgtggtggca gcgcccgcgc tgctggatgg tttcgccaag atcaagatgg
    13801 ccatggggct gaccacaccc gaaatgatgg aacgcgcggt gtaccagctt ttgcgcctgg
    13861 ggcatcatca tgcgcatttg cggcgggtgc aggacaggct ccgtgaggcg catgacgcgt
    13921 tgtgcgattt gctggagcgc catggcttcg aggtgttctg ccggccgcgg gccgggttgt
    13981 ttctttgggc gcggccgggc ggggaatggc gtgaacgcgg cgcggcgaga ttggcggaac
    14041 tggcactgaa ggacggaatc tggctggcgc cggggtcgta ctttcatccc gagcaactgg
    14101 atgagggatg gatccggttc aatgtggcgt attcgctgga tgcgtcgttg tggcggttca
    14161 tgcggaaagt gggggcgacc gtgtgagtgt catcgacgcc gatacacgac gaaatcaaag
    14221 gcgagcccgc tttcttcggg ttgccgttga cgttcgacct cttcccactg gtccggcggc
    14281 agagcgggaa agaacgcgtc cccgtcgacg tgcgcatgaa cctccgtcgc gatcacttca
    14341 tcggccatgg gcaacacctg cgcgtaaatc tgggcgccgc cgatcacgaa caccgttgct
    14401 tcgtcctggc agtcgttcag tgcatcccgc agcgatgggt aggccgatgc gccgtcggcc
    14461 ctcaggccgg gattgcggct gatgaccaga ttgcggcgtc ccggcagcgg cctgcccagg
    14521 gagtcccagg tgttgcggcc catgaggatg gggcatccca tggtcgtgtt cttgaaatgc
    14581 gcgaggtccg ccttcaggcg ccagggcagg gcgttgtcgc gtccgatggc gcggttgtcg
    14641 gagtaggcga cgacgagttt gatggtgggg cgcgtcatgg gattctggtc tctcgtctca
    14701 cacggccacg ggcgccttga tgtgcgggtg cgcctggtag tcgaggatct tgaaatcgtc
    14761 gtactcgtac tcgaagagag agggcgggcg gcgccggatg ttcaggcgcg ggtaggggaa
    14821 tggcgtgcgt gacagttgca ggcgcgcctg ttcgaagtgg ttggaataca ggtggcagtc
    14881 gccgcctgtc cagacgaagt cgcccacctc caggtcgcat tgctgggcga ccagatgggt
    14941 cagcagcgca tagctggcga tgttgaacgg gacgcccagg aaaatgtcgg cgctgcgctg
    15001 gtagagctgg cacgacagct tgccgttggc gacgtagaac tggaacagtg catggcaggg
    15061 cggcagcgcc atgtcgggga tgtcgcccac gttccaggcc gacacgatga ggcgccggga
    15121 atccggtgtt tcacgaattt gccgcacgat ctgggagatc tggtcgatgt gcccgccgtt
    15181 gcgcgtgggc caactgcgcc attgggcacc gtacaccggg cccaggttgc cgtcggcgtc
    15241 ggcccattcg tcccagatgc taacgccgcg ctcctgcagc cagcgcacat tggaatcgcc
    15301 ccgcagaaac cacagcagct cgatgaagat gcttttggtg tgcagcttct tggtggtgat
    15361 cagcggaaag ccttcgttca ggtcgaaacg catttggtgg ccgaacaccg agcgcgtgcc
    15421 ggtgccggtg cggtcggtct tggtctggcc gtgttcatac acgtgccgca tgaagtcttc
    15481 gtattgctgc ataaatatgg ctgtgcgcgt tgtattcagg ttcggtgccc gggcagggtc
    15541 gtccggttgg tcggggcctt ctggaaggcc acgccgcgaa cggggtttca ggcctcgacg
    15601 atctcggccg accaggtgat ttccgccgtt ttcgccagca tggcggacgc cgagcagtat
    15661 ttttcgtgcg agagccgcac ggcgcgctcg accgcgtcgc gcgtgaggcg cgaccccgtt
    15721 accgtgaagg cgaagtggat cttggtgaac accttggggt cggtatcggc acgctcgctg
    15781 gtcagtgtaa cccggcagtc ggtcaccgcg tggcgcccgc gttggaggat caggaccacg
    15841 tcgtaggcgg tgcaggcgcc ggtgccgcac agcaccattt ccatggggcg cggcgccagg
    15901 ttgtggccgc ccccgtcagg ggcaccgtcc atggttgcga tgtgtccact gcccgtgcgg
    15961 gcgacaaaga gcatgccgtc ggggccaccc cagtctatcg tgcattccat agtgtcttcc
    16021 tttgtgtggt gagcgcagac agcgcttcgt ccgggtcgtc gtatttttac actctttggc
    16081 cggtcagagc gtggcggttc cgcggaaatg cgttaacctg attctgcgta agggtatcag
    16141 gccgtggagg tgcgcgt
//
LOCUS       DASTNK010000081        12517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_242064, whole genome shotgun sequence.
ACCESSION   DASTNK010000081 DASTNK010000000
VERSION     DASTNK010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12517
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242064"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(289..978)
                     /locus_tag="VFR20_06355"
     CDS             complement(289..978)
                     /locus_tag="VFR20_06355"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEU0230173.1"
                     /translation="MTFLAALFDLDGTLLDTLDDLTDAVNAMRADYGQPALTRAVVET
                     YVGKGTDYLVMRSLAHDVLNQPPTPDDISEALKRFTSHYRRLCGDRATLYPGVVEGLA
                     AFRDRGLKLAVVTNKPTEFTGPLLEHMGIAHFFEHLVCGDTCARKKPDPMPFLHACSL
                     LGVEPGSALAVGDSINDAQAARAAGITVLAVPYGYRGGQDVRTLPVDGIVTSIENAAH
                     WAAQHQERSPS"
     gene            complement(992..1690)
                     /gene="rpe"
                     /locus_tag="VFR20_06360"
     CDS             complement(992..1690)
                     /gene="rpe"
                     /locus_tag="VFR20_06360"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU0230174.1"
                     /translation="MTHATTRIAPSLLSADFSRLGEEIRNVVDAGADWIHFDVMDNHY
                     VPNLSIGPMVCEALRPHTTVPIDVHLMVEPVDAIIPQFAKAGANIISFHPEATRHPDR
                     SLAHIKDNGCKAGMVFNPATSLDWMDYVMDKLDVVLLMSVNPGFGGQRFIPSTLGKLA
                     EARRKIDAWMRDGGQPIALEVDGGVKIDNIADIRAAGADTFVAGSAIFGQPDYRSTIA
                     QLRAEITRGESISV"
     gene            1827..2201
                     /gene="apaG"
                     /locus_tag="VFR20_06365"
     CDS             1827..2201
                     /gene="apaG"
                     /locus_tag="VFR20_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEU0230175.1"
                     /translation="MKPYDVVVVVTPQYLADQSEPADRQFLFAYTVRITNQGTHPAQV
                     ISRHWIITDGEQNVQEVRGLGVVGQQPLLGPGETFEYTSGCPLPTPVGTMQGTFLCVG
                     EDGIPFDAPIAEFVLAMPRTLH"
     gene            2244..3533
                     /locus_tag="VFR20_06370"
     CDS             2244..3533
                     /locus_tag="VFR20_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014486104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEU0230176.1"
                     /translation="MKRLLSPLLLLPVLLAACSTVPPRAVAPEAPQASSAASLALRPL
                     VVPPPGAFHDTRARALAARFHKTSWAELPGWNTDDLDHAWKAFINNCKGLMRPVSGSL
                     NVPARATPLVWRPVCAEAASSGISLTAPDAAAVRAFLQRTLQPWRVLDAAGAPARSLV
                     TGYYEPLVQASRERGGAYQWPLYAPPDDLLTIDLGSVYPALVGKRVRGKLVGKRVVPY
                     DTRAQIAADPKKPPVIVWVNDPVDAFFLQVQGSGRARLPDGAVVRLAYADHNGRPYLS
                     IGRWLAEQGQMPLSQASMQNIKAWARSHPDRVQEMLDANPAVVFFKEESIVDPDLGPA
                     GSYGVPLIGGRSIAVDASFVPLGTPVFLATDYPGTGRPLDRLVFAQDTGTAIRGAGRA
                     DYYWGFGSNAGELAGRMKQRGEMWVLWPKQAGAPSAR"
     gene            3530..4711
                     /locus_tag="VFR20_06375"
     CDS             3530..4711
                     /locus_tag="VFR20_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU0230177.1"
                     /translation="MNNESIVVCGSGIVGLAAALSLSRAGFHTSLVGPIEAVPPAAPD
                     TYCPRVYALSPASRRFLEALGVWQMLDSARISAVESMEVFGDAGGQVGLQAWQGVQPA
                     LAWIVESSELERVLQQAVRIGGVVWRDEKLEHAAPGVVVTEAGRSLPADLVVGADGAT
                     SRVRQAFAIAHQSRPYGDTGLVAHLNTGRPHQNAAIQWFTGDSVLALLPMPNTGDGSQ
                     VSMVWSLPAADADRLRAAARDGRVAALEARLAATTGGRLGRLALRGPVFGFPLFVENS
                     GMVAPGAALVGDAAHRVHPLAGQGLNLGLADVCALVEALTAREPYRRVGDSRVLQRYR
                     RMRAEPLLSMKLATDGLYRLFALKGAPAAMARNAGMRIVDHLPMVKRHLIEAASGWPD
                     Y"
     gene            4749..5534
                     /locus_tag="VFR20_06380"
     CDS             4749..5534
                     /locus_tag="VFR20_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEU0230178.1"
                     /translation="MRISILAATLFIAALGAPGVGHAAPAAASTNNVSMTAQGESVPV
                     SLGNSTLDGIRKMFESRFPGVEVAGVRPTPFKGLYEIQIGMDVIYTDAKVDYVLQGSL
                     VDAKTRTDLTAARLAKLAEVPFDSLPLDLAIKQVRGNGQRKMAVFEDPNCGYCKLLHR
                     TLQQVDNVTVYTFLFPVLAPDSIVKARDIWCAKDRARAWEDWMVDGKAPADATCDTPI
                     NKILALGRKLMVQGTPAIIFADGSRVNGTLPLAALNEKLASLR"
     gene            5644..6111
                     /locus_tag="VFR20_06385"
     CDS             5644..6111
                     /locus_tag="VFR20_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0230179.1"
                     /translation="MTATQIKLAPPDDPLRLDGQLCFALYSAGLAMNKVYRRLLKGLG
                     LTYPQYLVMLVLWEQDEITVSEIGRRLYLDSATLTPLLKRMETARLIQRARAVSDERQ
                     VIVSLSAKGRKLRDDALDVPAQALCAANVVSPRDAAGLVGELGRLRQSLMSNF"
     gene            complement(6130..6675)
                     /locus_tag="VFR20_06390"
     CDS             complement(6130..6675)
                     /locus_tag="VFR20_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HEU0230180.1"
                     /translation="MALNATIHKAELHIADNDRGYYGSHAITLARHPSETDERMMIRL
                     LAYALYEKGDDLLSFTRGLSDNDEPDLWRRDLTGIIEQWVEVGLPDARRLLKACGRAQ
                     EVIVFAYGRSVATWWSDVAPKVARARGLKVFTLPVAGTQALAALAERTMTLNINIQDG
                     TIWVSGHGGEANLEVARLHPA"
     gene            6874..7416
                     /locus_tag="VFR20_06395"
     CDS             6874..7416
                     /locus_tag="VFR20_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230181.1"
                     /translation="MTRISIIVAAATTAALSGCAAMTAGQLVQAGYDAAKTSLTSAPG
                     NQATALHVRTIMNSINVGQDTASIIASIGEPPMEKSGNPQGYTCYEYAAVYSPTEDAV
                     IVSKDGKVVFYGDSTCRVEMRDDNFRAGGKYAGPPGGESSAGSGAGQGSAPDQVKGSV
                     SNPAATSGAVSAPDTTPQQK"
     gene            complement(7454..8452)
                     /locus_tag="VFR20_06400"
     CDS             complement(7454..8452)
                     /locus_tag="VFR20_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU0230182.1"
                     /translation="MNRRRLLQATALGAFSTVLAPTSNAQGRSAALPDLKKAITDFTT
                     LSPGRAAACISIEEDNGSRSTVHAADPDQPLFVGSAVKTFILAQYLREVEAGRLSEDT
                     QVAVGPEVWSPGSPVFMHLTGTTPARSALEAMIAHSDNTGTDIALNAAGADQVRQLIK
                     TAGLTHTLIPDSTRKLFSYLAGAPNGVDIGWAGMEKMAKDIMPGKPRKPVNDHQTMMS
                     TAGELTRWYEQALAGKYFKKHETLAEFKRISAMANAMPMMVPEDVMAYGKGGSIDWGD
                     FHCFCGAGQMVQTSRRTSFCFILNWTGADGTVPTMLESFKKQSRHVLSLAANSRAA"
     gene            8766..9737
                     /locus_tag="VFR20_06405"
     CDS             8766..9737
                     /locus_tag="VFR20_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU0230183.1"
                     /translation="MVRIRAFEDAAEIVSQGGVQAWGQEASAKPALVKGPLHLSTGQE
                     AVPSGVCAHLTHHDLLTSTHRGHGHTLAKGADPVRMMCELFGRAGGYNKGKGGSMHIA
                     DFAVGMLGANGVVADGLPIAVGAAHALKLRGEDAIVTSFFGDGAINRGPFLEALNWAV
                     VYKLPVLFVCEDNRISATTPSEAMTGGDGASARAESIGVAALRVDGNDVEAVSALAES
                     MVADVRAGRGPCLLHAITYRHKGHVSIDPGAYRDPAEVRAAMEQDPIQRARRRLDELG
                     QAREADGIERAAHAEIQAALQIAEAAPFPVPADAYEDIITTGAGVWS"
     gene            9734..10729
                     /locus_tag="VFR20_06410"
     CDS             9734..10729
                     /locus_tag="VFR20_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU0230184.1"
                     /translation="MNKPVEVSASVLTYAQAGALALAEAMQADPAIVALGEDLGRGGV
                     FGQYRDADGAPLVKRFGPQRIIDTPISEAAIVGAGVGMALAGLRPVVELRVVDFALCA
                     IDELVNQAAKNRFMFGGQGRVPMVARLPIGIWTASAAQHSQSLEAWFAHIPGLVVACP
                     ATPADNYGLLRAALDCGDPVIYMEHKELWGSQGPVVPGEPVALGKGRVARAGEDITLV
                     SWSKQVWAACEAADTLAREGVRAEVIDLRTLWPWDKDLVLASAARPGRVLVTHEAVQV
                     AGFGAEVAATVAEHTGARVARLGAPRIPVGYSPVLENESRISADKIVSAVRKLLG"
     gene            10881..11327
                     /locus_tag="VFR20_06415"
     CDS             10881..11327
                     /locus_tag="VFR20_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230185.1"
                     /translation="MNRKYRFAAATILGVAMLPLGASYAASDDPATQFVAGVQAGLLD
                     GAGVVTGLDSYGGVPHASAFEQRGLENMVGVLNYCLKARVASGVRNPRQADATVDNLG
                     EPDFERVADAGNPDGLWQQSVPDVAAVGEAPPQICRVVRMSVRSRW"
     gene            complement(11384..>12517)
                     /locus_tag="VFR20_06420"
     CDS             complement(11384..>12517)
                     /locus_tag="VFR20_06420"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HEU0230186.1"
                     /translation="TWIATLGLASAMGIGRFAFTPLLPMMQQSLGLSLRHGAWLASAN
                     YIGYFVGALACLVFNPRPGRAARLSLFAIAILTMAMGLTHALDAWLVLRLLAGVASAY
                     ALIGISSWILALLEQQRKPGLAGRAFGGVGLGIMVAGLMCLVAAVWRQSPGHTWITLG
                     AVALAVFVIAWVPLGWSDDVGSATDAAPPRRRLTSDEWMLALCYGIFGLGYIIPATFL
                     PAFARQLINDPAVFGWVWPAFGLAALLSTIVVANLFRHTPPRTTWAVCHLIIAIGVLA
                     PALSPRMTAILFSTVCVGGTFMVITMEGMREARRVAGASAPRLIAAMTAAFALGQLIG
                     PLTIPAGPFEEAIFVPSLATASLLLLTVLMLMLMRGRAAPTAT"
BASE COUNT         2099 a         3800 c         4250 g         2368 t
ORIGIN      
        1 tcggcatagg tctcggcgat gatggggata cggttgtagc cttcggcggc caatgcgttg
       61 aattcgattt ctgtcatgga gggtctcggg ttattggtgt tatttccgcg ttgaccgggc
      121 aggaatcaaa aaagcccggc cattgggtgg gtccgggctt ttgctggcta gactgacggc
      181 gtgcttgaac aatgtcaggc atgccgggag aggatcaccg ccacccggag cacatacgcc
      241 accagcgcca cgaggcgcaa ggtgttgcgt aggaataggg ggtcgcggtc atgatggtga
      301 tcgttcctgg tgttgcgccg cccagtgtgc ggcgttttcg atggacgtaa ctataccatc
      361 gaccggcagg gtccgcacgt cctgtccgcc acggtatccg tagggcacgg ccaggacggt
      421 gatgccggcg gcgcgcgccg cctgggcgtc gttgatcgaa tcgcccacgg ccaatgcgct
      481 gcccggttcg acgccaagca gcgagcaggc atgcaggaat ggcatggggt cgggcttttt
      541 gcgggcgcag gtgtcgccac acaccagatg ctcaaagaag tgggcgatgc ccatgtgttc
      601 gagcagcggg ccggtgaact cggtgggctt gttggtgacc accgcgagct tcaagccccg
      661 gtcgcgaaac gccgccaagc cttcgacgac gcctggatag agggtcgcgc ggtcaccgca
      721 caggcgccgg tagtggctgg tgaagcgttt cagtgcttcg ctgatgtcat ccggcgtggg
      781 gggctggttg agcacgtcgt gtgccaggct gcgcatgacg agatagtcag tgcccttgcc
      841 cacgtaggtc tcgacgaccg cgcgtgtgag ggctggttgc ccgtagtcgg cgcgcatggc
      901 gttgaccgca tcggtcaggt catcgagcgt gtcgagcaga gtgccgtcca ggtcgaacag
      961 cgcggcgaga aaagtcatgg atagcccagc cctagacgct gatgctttcg ccgcgcgtga
     1021 tctcggcgcg cagctgggcg atggttgagc ggtaatcggg ttgtccgaag atggccgagc
     1081 ccgctacgaa ggtatcggca cccgcggcgc gtatgtcggc gatgttgtcg attttcacgc
     1141 cgccatcgac ctccagggcg atgggctggc cgccatcacg catccaggcg tcgatcttgc
     1201 ggcgcgcctc ggcaagcttg cccagcgtgg atggaatgaa gcgctggccc ccgaaccccg
     1261 ggttgaccga catgagcagc accacgtcca gcttgtccat gacatagtcc atccagtcca
     1321 gcgacgtggc gggattgaag accatgccgg ccttgcaccc gttgtccttg atgtgcgcca
     1381 gggagcggtc ggggtgacgg gtggcttccg gatgaaagga aatgatgttg gcgcccgcct
     1441 tggcgaattg cgggataatg gcgtcgaccg gttcgaccat caggtgcaca tcgatgggaa
     1501 cggtggtgtg cgggcgcagg gcttcgcaca ccatggggcc gatcgacaga ttgggaacgt
     1561 agtggttgtc catgacgtcg aagtggatcc agtcggcgcc ggcatcgacg acgttgcgga
     1621 tttcctcgcc caggcgggag aagtcggccg acagcaggct gggggcgatg cgggtggtgg
     1681 cgtgtgtcat ggcgtacgat gtcgtttggt gttcggcgat ggaatcggaa ataatgtcac
     1741 attattgcag gttggcttcg ggttttcctt ccggatcccg gcgagttcca tcaagcgtca
     1801 aacactacac tggcaggaac cctctcttga aaccgtacga tgtcgtcgtg gtcgtcacgc
     1861 cccaatatct ggcggatcag tccgagcccg ccgatcggca attcctgttt gcctatacgg
     1921 tgcgcatcac caaccagggc acgcatccgg cacaggtcat cagccgccac tggatcatca
     1981 ctgatggcga gcagaacgtc caagaggtgc gcggcctggg ggtcgtgggc cagcagccgt
     2041 tgctggggcc tggcgaaaca ttcgaataca ccagcggctg ccccctgccc acgccggtcg
     2101 gtaccatgca aggcacgttc ctgtgcgtgg gtgaggacgg cattccgttc gacgccccca
     2161 tcgccgagtt cgtcttggcc atgcctcgca ccctgcattg aaagcatcgc gattccgcgc
     2221 cccctcattt tttcgtgtgt ttcatgaaaa ggcttcttag tcctctgttg ctgctgccgg
     2281 ttttgctggc ggcgtgcagt accgttccgc ctcgggcggt ggctcccgaa gcgccccagg
     2341 cgtcctccgc cgccagcctg gcgttgcgcc cgctcgtggt gccgcctccc ggcgcgttcc
     2401 atgacacacg ggcgcgcgca ttggcggctc ggttccacaa gacgtcgtgg gcggaactgc
     2461 ctggttggaa cacggatgac ctggatcatg cctggaaggc attcatcaat aattgcaaag
     2521 ggctgatgcg cccggtgtcc gggtcactca atgtgccggc gcgcgccacg ccgctggtct
     2581 ggcgcccggt gtgcgccgag gccgcgtcat ccggcatctc gctcaccgcg cccgatgccg
     2641 cggcggtgcg ggcgttcctg caacgcacac tgcagccctg gcgtgtgctc gatgccgccg
     2701 gcgcaccggc gcgcagtctg gtcactgggt attacgagcc gctggtgcaa gcgtcccgcg
     2761 agcgcggggg cgcctatcaa tggccgctgt acgcgccgcc ggatgatctg ctgaccatag
     2821 acctggggtc ggtgtatccg gcactggttg gaaaacgggt gcgcggcaag ctggttggca
     2881 aacgcgtggt gccctacgac acgcgggccc agatcgccgc ggatcccaag aaaccccccg
     2941 tcatcgtctg ggtcaatgat cctgtggatg cgttcttcct gcaggtgcag ggttcgggcc
     3001 gcgcccggtt gccggatggc gcggtggtgc gactggccta cgccgaccat aacggacgcc
     3061 cgtacctgtc catcggtcgc tggctggccg agcaaggcca gatgccattg tcccaggctt
     3121 cgatgcagaa catcaaagcc tgggcgcgaa gccatcccga ccgtgtccag gaaatgctcg
     3181 atgcgaatcc agcggtggtg ttcttcaagg aagagtccat tgtcgatccg gaccttggcc
     3241 cagcgggctc atatggcgtg ccgttgatcg gaggccgctc catcgcggtg gatgcgtcgt
     3301 tcgtgccgtt gggcacgcct gtgttcctcg ccacggacta ccccggcacg ggcaggccgc
     3361 tggaccgtct ggtgttcgcg caggatacgg gtaccgccat acgtggcgcc ggccgggccg
     3421 attattactg gggtttcggc agcaatgccg gggaactcgc cgggcgcatg aagcagcgcg
     3481 gcgaaatgtg ggtgctgtgg ccaaaacagg cgggggcgcc gtcggcacga tgaacaacga
     3541 atccattgtc gtatgcggta gcggaatcgt cgggctggcg gcggcgctgt cgttgtcccg
     3601 agcggggttc cacaccagcc tggtggggcc gatcgaagcg gttccgcccg ccgcgccgga
     3661 tacctattgt ccgcgcgtgt atgccctgtc gccggccagt cgacgcttcc tcgaggcgct
     3721 gggcgtctgg caaatgctcg attccgcccg gatctcggcg gtcgaatcca tggaggtgtt
     3781 cggcgacgcg ggtggccaag tggggttgca ggcctggcag ggtgtgcagc ccgcgctggc
     3841 ctggatcgtc gaatccagtg aactcgagcg cgtgctgcaa caggcggtgc gcatcggtgg
     3901 cgtcgtctgg cgcgacgaga aactcgagca cgccgccccg ggtgtcgtcg tcaccgaagc
     3961 tgggcgttcg ttgcccgccg acctggtggt cggcgccgat ggtgccacgt cccgggtgcg
     4021 tcaggctttt gccatcgcgc atcaatcccg gccctatggc gacaccgggt tggtcgcgca
     4081 tctcaacacc gggcgcccgc accagaatgc cgccatacag tggtttaccg gcgacagtgt
     4141 gctggcgctg ctacccatgc cgaacacagg cgatggcagc caggtttcca tggtgtggtc
     4201 tctgccggcc gccgacgctg atcgcctgcg ggctgcggcg cgggatggcc gcgtggccgc
     4261 cctcgaggct cggctggcgg ccacgaccgg agggcggctg ggaaggttgg cgttgcgcgg
     4321 ccccgtgttc gggtttccgc ttttcgtgga aaacagtggt atggtggctc ccggcgcggc
     4381 actggtcggc gacgcggcgc atcgtgtgca tccgctggcg ggccaggggt tgaatttggg
     4441 gctcgccgat gtgtgcgccc tggtcgaggc gctgacggca agggaacctt atcgtcgcgt
     4501 tggcgatagt cgcgttctgc agcgttaccg acgcatgcgc gccgaaccgt tgctgtccat
     4561 gaagctcgcc accgatggct tgtaccggtt gttcgcgctg aagggcgcgc ccgcggccat
     4621 ggcgcgcaac gccggcatgc gtatcgtcga tcatttgccc atggtcaagc ggcacctgat
     4681 cgaggccgcg tcgggctggc ccgattactg acaacaatgc gccgcgtggg gcggcactgg
     4741 agtttttcat gcggatttca attctcgcag cgaccctgtt catcgccgcg ctgggtgccc
     4801 cgggggtggg ccacgcggcg ccagcggctg catcgaccaa caatgtatcg atgacggcgc
     4861 agggtgagtc ggtgccggtc agcctgggca acagcacact ggatggcatc aggaaaatgt
     4921 tcgaatcccg tttccccggt gtcgaggtgg ccggggtgcg gcccacgccg ttcaaaggtc
     4981 tgtacgagat ccagatcggc atggatgtga tctataccga cgccaaggtg gattatgtcc
     5041 tgcagggttc gcttgtcgac gccaaaacac gaaccgatct gacggccgcg cggctggcca
     5101 aactggccga ggtgccgttc gattccctgc ccctggatct ggccatcaag caggtcaggg
     5161 gaaacgggca gcgcaagatg gccgtctttg aagaccccaa ttgcggctac tgcaaattgc
     5221 tgcaccgcac tttgcaacag gtcgacaacg tcacggttta tacattcctg tttccggtcc
     5281 tggcccccga ttccatcgtc aaggcccgcg acatctggtg tgccaaggat cgcgcgcgcg
     5341 cgtgggaaga ctggatggtc gacggcaagg ccccggccga tgccacctgc gacactccca
     5401 tcaacaagat cctggcgctg gggcggaaac tgatggtgca ggggacgccc gccatcattt
     5461 tcgccgatgg cagccgtgtc aatggcacgt tgccgctggc cgcgctgaac gagaagcttg
     5521 cgtcgctacg ttgaatggcg acactggcaa gacggtggtg ccgttcgcag tctgtagcgt
     5581 catgttgcac aatgtgtgga aatttccggc aacggcatcc gcgcaataga ataaattaca
     5641 accatgaccg ccacccagat caaactcgcg ccacccgacg acccgctgcg gttggacggg
     5701 cagttgtgct ttgcgctgta ttcggcgggt ctggccatga acaaggtcta ccggcggctg
     5761 ctcaagggcc tgggcctgac gtacccgcag tatctggtga tgctggtcct gtgggaacag
     5821 gacgaaatta ccgtgtcgga aatcggtcgg cggctgtatc tggattccgc caccctgacg
     5881 ccgttgctca agcgtatgga aaccgcccgc ctcattcagc gtgcccgcgc ggtgtccgat
     5941 gagcgccaag tcattgtttc cctcagcgcg aaaggccgca agctgcgcga cgatgcgctg
     6001 gatgtgccgg ctcaggcgtt gtgcgccgcc aatgtggtgt cgccgcgtga cgcggccggg
     6061 ttggtcggcg aactcgggcg gttacgccag agcctgatgt ccaacttctg acggtttccc
     6121 cctgggctgt caggccgggt gcaggcgcgc cacttccagg ttcgcctccc cgccgtgccc
     6181 cgacacccag atcgtgccgt cctggatgtt gatgttcagt gtcatggtgc gctcggccaa
     6241 ggccgccaaa gcctgggttc cggcgacggg caatgtgaag accttcagtc cgcgcgcacg
     6301 ggccaccttc ggcgcgacgt ccgaccacca cgtcgccacg ctgcgcccat aggcgaacac
     6361 aatcacttcc tgtgcgcgtc cgcacgcttt gagcaagcgg cgcgcgtcag gcaggccgac
     6421 ctccacccat tgttcgatga ttcccgtcag gtcgcggcgc cacagatcag gctcatcgtt
     6481 gtcgctcaac ccccgcgtga acgacaacaa atcgtcgccc ttttcataca gcgcgtaggc
     6541 gagcaggcgg atcatcatgc gctcgtccgt ttccgatgga tgccgggcca gcgtgatggc
     6601 gtggctgcca tagtagccgc ggtcgttgtc cgcgatgtgg agttcggctt tgtgaatggt
     6661 tgcgttcagg gccatgttcg tgtgtgtccg gtggttcggg cgattgtagg gcaacacttg
     6721 tccgccacgc gttcatgggc ggtaaaatcc gcgtgatcac cgaggcacag gtcccgttgt
     6781 gcacctaatg ccctgaattt caatgctcga atcaaccgcg cggtacgtcg tgcttcgggc
     6841 gaatcatgtg atattccagg agtcttcatg cggatgacgc gtatttccat catcgtggcc
     6901 gcagcgacaa cggcggcgct gagcggatgc gccgcgatga cagcgggcca gctcgtgcag
     6961 gcggggtatg acgcggccaa gacgtcgttg acgagcgctc cggggaatca ggcaaccgca
     7021 ttgcatgtgc ggacgatcat gaactcgatc aacgtcggcc aggacactgc atcgatcatc
     7081 gcgtcaattg gcgagccacc aatggaaaaa tcaggcaatc cgcagggcta cacctgttac
     7141 gagtacgcgg ccgtgtactc cccaacggaa gacgccgtca ttgtctcgaa ggatggcaag
     7201 gtggtgtttt atggtgattc cacgtgtcga gtcgagatgc gggatgacaa cttcagggcg
     7261 ggggggaaat atgcgggccc ccccggtggc gaatccagtg ccggatcggg cgcgggtcag
     7321 ggttccgccc ccgaccaagt caagggttct gtttccaacc ccgctgcgac gtccggcgcc
     7381 gtttcggctc cagacaccac gccgcaacaa aagtgaccga caagggctgg ctggaaatca
     7441 cgccacacga tcattacgcg gcgcggctgt ttgccgccag actcagtacg tgtcggcttt
     7501 gtttcttgaa cgattcgagc attgtgggaa ccgtgccgtc ggcccctgtc cagttcaaga
     7561 tgaagcagaa cgaggtgcgc cgcgaagttt ggaccatctg gcctgcgccg cagaaacaat
     7621 ggaagtcgcc ccagtcgatg ctgccgccct tgccataggc catgacatcc tccggcacca
     7681 tcatgggcat ggcgttggcc atcgccgaga tgcgtttgaa ttcagccagg gtttcgtgtt
     7741 tcttgaagta cttgccagcc agggcctgtt cataccagcg ggtcagctcg cccgccgtgc
     7801 tcatcatggt ctgatgatcg ttgacgggct ttctgggttt gccgggcatg atgtccttgg
     7861 ccattttttc catgcccgcc cagccgatgt cgacaccgtt gggcgcgccg gcgagatatg
     7921 aaaacagttt gcgggtggag tcaggaatga gggtgtgtgt cagacccgct gttttgatca
     7981 attggcggac ctgatccgcc cccgcggcgt tcaaggcgat gtcggtgccg gtgttgtcac
     8041 tgtgggcgat catggcctcc agggcgcttc tcgcgggggt cgtgccagtg agatgcatga
     8101 ataccgggct gcccggcgac cacacttcgg ggccgacggc aacctgggtg tcttcggaca
     8161 gtcggcccgc ttcgacttcc ctcaggtatt gcgccagaat gaatgtcttc accgcgctgc
     8221 cgacgaacag cggttgatcg gggtcggccg catggaccgt gcttctactt ccattgtctt
     8281 cttcgatcga tatgcaagcg gccgcccgtc cgggggacag cgtggtgaaa tccgtgatgg
     8341 cttttttcag atcaggcaat gcggcggatc ttccctgtgc gttgctggtc ggtgcgagaa
     8401 ccgtggaaaa tgcgcccagg gcggtggctt gcagcagacg tctgcgattc atgtgtgtct
     8461 ccgggatttt tgtcatgaag ccgaccccgg ggtcacgatg gccttgcggg gacaggcggt
     8521 gttcgtgatg ctcatgttct gtgatagtag ctgaaccgaa acagggatgc gtcgcgaaca
     8581 cgcgatgtgg agatccgttg gcgcgcgttg gggtgatcgc cgcgcgacct gtcccccggt
     8641 cgtttgagcc ggatcaggga tttgctatgc tgtcctcttt ccatgcgtgg ggcgagatca
     8701 tcatgaatgg ttcatcagtg ccggatccat caaccgccac gcggctgctt ggcttgtatc
     8761 gcagcatggt gcgcatccga gccttcgagg atgcggccga gatcgtcagc cagggcggcg
     8821 ttcaggcctg gggccaggag gcctcggcca agcccgcgct ggtcaagggg cctttgcatc
     8881 tgtccaccgg ccaagaggcc gtgcccagcg gtgtgtgcgc gcatctcacc catcacgacc
     8941 tgctcacttc gacacatcgc gggcacggtc acacgttggc caagggggcc gaccccgtcc
     9001 gcatgatgtg cgagctcttc ggtcgggcgg gcggctacaa caaaggcaag ggcggttcca
     9061 tgcacatcgc cgatttcgcc gtgggcatgc tgggtgccaa tggcgtggtg gccgacgggc
     9121 tgcccatagc ggtcggcgcg gcgcatgcgc tcaagctgcg tggcgaagac gccatcgtca
     9181 cgtcattctt cggtgacgga gcgatcaacc gggggccgtt tctcgaggcg ttgaactggg
     9241 ccgtggtcta caagttgccg gtcttgttcg tgtgtgaaga caaccgcatt tccgccacca
     9301 cccccagcga ggccatgacg ggtggtgatg gcgccagcgc ccgggcggaa tccatcggtg
     9361 tcgcggcgtt gcgcgtcgac ggcaatgatg tcgaggcggt gtcggccctg gctgaaagta
     9421 tggttgccga cgttcgggcg ggtcgcggcc cgtgcctgct gcacgccatc acgtatcgcc
     9481 acaaagggca cgtgtccata gatcccggcg cgtaccgcga tccggccgag gtgcgcgcgg
     9541 cgatggaaca agatcccatc cagcgcgcgc gtcgccggct tgatgaactg ggccaggccc
     9601 gcgaggcgga tggcatcgaa cgcgcggcgc atgccgagat ccaggcggcg ctccagatcg
     9661 ctgaagcggc gccgtttccg gtgccggccg acgcctacga agatatcatc accacaggag
     9721 cgggggtctg gtcatgaaca aaccagtgga agtgtccgcg tcggtgctca cctacgcgca
     9781 ggccggtgcg ttggctctgg ccgaggcaat gcaggccgac cccgccatcg tggccctggg
     9841 ggaggatctg gggcgtggcg gtgtgttcgg gcaataccgc gatgccgacg gcgcgccatt
     9901 ggtgaaacgc ttcggcccgc agcgcatcat cgacaccccc atttccgaag ccgccatcgt
     9961 cggggccggt gtgggcatgg cgttggcggg gctgcgcccg gtggtcgaac tgcgggtggt
    10021 ggatttcgcc ttgtgcgcca tcgatgaact cgtgaaccag gcagccaaga accgcttcat
    10081 gttcggcggg caaggcaggg tgccgatggt ggcccggttg cccatcggca tctggaccgc
    10141 atcggcggca cagcattccc agtcgctgga ggcctggttc gcgcacattc ccggcctggt
    10201 cgttgcctgc ccggccacgc ctgccgacaa ctacgggttg ctgcgcgcgg cgctcgattg
    10261 cggcgacccc gtcatctaca tggaacacaa agagttgtgg ggcagtcagg gcccggtggt
    10321 tcccggcgag ccggtggcgc tgggaaaagg gcgcgtcgcg cgggccggtg aagacatcac
    10381 cctcgtcagt tggtcgaaac aggtgtgggc ggcctgcgag gcggccgaca cgttggcgcg
    10441 cgagggggtc cgcgccgagg tcatcgactt gcgcacgttg tggccctggg acaaggatct
    10501 ggtgctggcg tcggccgcgc gccccggccg ggttctggtt acgcacgagg ccgttcaggt
    10561 ggcgggtttt ggcgccgaag tggctgccac cgtcgcggag cacaccggcg cgcgagtggc
    10621 ccgtctgggg gcgccgcgca tcccggtcgg ctattccccc gtgctcgaga acgaatcgcg
    10681 catcagtgcg gacaagatcg tgtccgcggt gcgcaagcta ttgggctagc atgattggcg
    10741 tggcgccagg ctggtggcgt cttgcctcca cccatcgggt atcgagcctc cgtcgcaaga
    10801 ggggtggcgg tcgactggcc gagtgttcaa aagcgcacta tagtgaacga taagcggttt
    10861 ttgtggaaga aaggtgcgtc atgaatcgaa agtatcgttt tgccgctgcg acgatcctgg
    10921 gtgtggccat gctccctctg ggcgcctcat acgcagcctc ggatgaccca gcgacgcagt
    10981 tcgtggccgg tgtgcaggcc ggcttgctcg atggcgcagg tgtcgtcacc ggcctggaca
    11041 gttacggcgg ggtgcctcac gcctcggcgt tcgaacagcg tgggttggaa aacatggtcg
    11101 gtgtgctgaa ttattgtttg aaagcgcggg tcgcttccgg ggttcgcaac cctcgtcagg
    11161 ccgacgccac ggtcgacaat ctcggcgagc cggatttcga acgcgtggcc gacgcgggga
    11221 atcctgacgg tttgtggcaa cagagcgtgc ctgatgtggc cgctgtcggt gaggcgccgc
    11281 cgcagatttg ccgcgttgtc cgcatgtcgg tgcggtcccg gtggtgacgc agggccgagc
    11341 ctgcccgtga cgaccgattc ggcgattgct gtatgccccc cggtcaggtc gcggttggcg
    11401 ccgcccggcc acgcatcagc atcagcatca gcacagtgag caacagtagg ctggcggtgg
    11461 cgaggcttgg gacgaatatg gcctcctcga aaggccctgc cgggattgtc aatgggccga
    11521 tcaattggcc gagggcgaac gcggcggtca tggcggcgat cagcctcggg gcgctggcgc
    11581 ccgccacgcg acgcgcctcg cgcatgccct ccatggtgat gaccatgaac gtgccgccca
    11641 cgcacactgt gctgaagagt atcgcggtca tgcgcggcga cagggccgga gccaggacgc
    11701 caatggcgat gatgaggtgg cacaccgccc atgttgtgcg tggcggcgtg tgccggaaca
    11761 ggtttgccac cacgatagtc gacagcaggg cggcaagccc gaacgctggc cagacccagc
    11821 cgaacaccgc tgggtcgttg atcagctgcc gcgcgaaggc gggcaggaaa gtagcgggaa
    11881 tgatgtagcc gaggccgaag atgccatagc acaacgccag catccattca tcggatgtca
    11941 ggcggcgtcg aggcggcgcg gcgtccgtgg ccgagccgac gtcatccgac caccccaacg
    12001 gcacccaggc gatgacgaac acggccagtg ccaccgcacc cagcgtgatc caggtgtgac
    12061 caggcgattg gcgccagacc gccgccacca ggcacatgag acccgccacc atgatgccca
    12121 gccccacccc gccaaaggcc cggcccgcca ggccgggttt ccgctgttgt tccagcaacg
    12181 cgagtatcca ggacgaaatg ccgatcagcg cataggcgct ggccaccccc gcgagcaggc
    12241 gaagaacgag ccatgcgtcg agagcatgag tcagtcccat ggccatggtc aggatggcga
    12301 tggcgaacag gctaagcctt gccgcccgcc ccgggcgtgg gttgaacacc aggcacgcca
    12361 gcgcgccgac gaaataaccg atgtagttcg cgctggccag ccaggcaccg tgtcgcagcg
    12421 acaggccaag gctttgctgc atcatcggca gcagcggcgt gaaggcgaag cgcccgatac
    12481 ccatggcgga cgccaggccg agcgtggcga tccaggt
//
LOCUS       DASTNK010000082        12711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_612730, whole genome shotgun sequence.
ACCESSION   DASTNK010000082 DASTNK010000000
VERSION     DASTNK010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12711
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612730"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VFR20_06425"
     CDS             <1..653
                     /locus_tag="VFR20_06425"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEU0230187.1"
                     /translation="QIRDGALHQGDKLPTESELMQLQGVSRTVVREAISRLQAGGVVE
                     TRHGIGSFVLGLPNEQSFHINPTDLVTIQDVLNVLQLRQSLESAAAGIAAQQRSQDDL
                     DTLAAILTEFRQCLDAGQDTVDVDFRFHLSIARATGNRYFVEFMSYLGQTVIPRTRIN
                     TASLGKETQQRYLERVHYEHEEIYKAILLQNPLVASSAMQMHLNNSAERLRSTLNH"
     gene            812..1723
                     /gene="kdgD"
                     /locus_tag="VFR20_06430"
     CDS             812..1723
                     /gene="kdgD"
                     /locus_tag="VFR20_06430"
                     /EC_number="4.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047448 - 5-dehydro-4-deoxyglucarate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxyglucarate dehydratase"
                     /protein_id="HEU0230188.1"
                     /translation="MDPQKLKTVLGSGLLSFPLTDFDTTGDFNEAGYRARLEWLIPYG
                     ATALFAAGGTGEYFSLSGDEYPAIIKAAVDTCRGHVPIIAGAGGPTRFAIECARAAER
                     AGADGILLMPPYLTETSQAGLAAHIEAVCRSVGIGVIVYHRANSLPTPDTLEQLAGRC
                     PNLIGFKDGVGDIEKMSAVYTRMGDRLAYLGGLPTAEVYAAAYKALGTPVYSSAVFNF
                     IPRTAMEFYRAVESDDLATQHQLLKTFFHPYLDIRNRGPGYAVSIVKAGAALVGHDAG
                     PVRSPLSDLTPKESRQLGELIERLGPQ"
     gene            complement(2007..3035)
                     /locus_tag="VFR20_06435"
     CDS             complement(2007..3035)
                     /locus_tag="VFR20_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007736104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU0230189.1"
                     /translation="MATSTFRKTRSTGMPTLAQVAQRAGVSTITASRALRGLSTVGPE
                     YVQRVKEAALALNYVANPAARALASACSAAVAVLVPSLSNTVFLDTLEAIHGVLAPRG
                     LELLIGNSHYSREAEEHLIRHYLASPPGGMIVTGFDHSEVARRLIDASGVPCVHIMEL
                     TNAVGVHCVGFSQAQAGAAVARHLIARGCRRNAFVAAQLDPRMLQRGEGFRDVLRAGG
                     LHDPGLEVLTTAPSSIGLGCELLRQLLERRPDVDGIFFGNDDLAQGALFEAQRLGIDV
                     PGQIAIAGFNDLAGAADCVPRLTTVRTPRAEMGRTAARLLLSMIDKEPIESPAIDLGF
                     ELIVRESA"
     gene            3200..3739
                     /locus_tag="VFR20_06440"
     CDS             3200..3739
                     /locus_tag="VFR20_06440"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEU0230190.1"
                     /translation="MALVVMGVAGCGKTSVAQAIVARMGGCLIEGDTFHPAASVEKMR
                     RGIALTDEDRGGWLDRLATELRDTARRDERAVLACSALKRRYRDQLRRGTPTLGFVFL
                     RLSQREALTRVTQRIGHYMPASLVASQFADLEPPVSEPLSLSVDATLPVGDIVDQVAR
                     WYAQTVTQEGFRAVTNVTH"
     gene            3933..5237
                     /locus_tag="VFR20_06445"
     CDS             3933..5237
                     /locus_tag="VFR20_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0230191.1"
                     /translation="MIGVLLGIGVLINYFDRINLSVAGPQLTQAFHLTPAELGLLFSA
                     FFWPYALLQIPAGLLLDRYGVTRIGRWGAFLWGLASGMMVLVSGFGGVFAARALLGVA
                     EAPGFPVSSKATGYWFPRKERALATAIFDAAAKFSNVIGVPLVALVVVKLGWRWGFGV
                     TATLSLLYFLAFYFIYQDPSKDTRLSQEERHYIVQGGATPEGPASTGSINMLGYLLTK
                     AKVWGLTIGFAAYGYSFYLFLTWLPEYLVQTMHMSILKSAEFAAIPWAVATVTDLVVG
                     GWLIDHLIARGRNETRVRKAVLVIGMLLGLAVFGATRTVTPGWAIFWISVALGGLATA
                     APVGWSLPSLIAPRGGVGTVGGIMNFVNNLMGVAAPVVTGFIVGATHSFTNAFTVAGV
                     VLAIGIVSFVFVMGRIEAIPEYSQAGDRKREPLATGGAGQAE"
     gene            complement(5315..6700)
                     /locus_tag="VFR20_06450"
     CDS             complement(5315..6700)
                     /locus_tag="VFR20_06450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0230192.1"
                     /translation="MSKQNSGSTGHKDASLAIGKVRWSIAVLLGVGILINYIDRLSLA
                     LVATPMSHDFGLTATDFGIIGSAFVWSYALMQIPSGILLDRMGAKWIWRIGMATWAIA
                     SFLTAVASGYWVVILARLLLGLAEAPAFPGAMKATGTWFPVTERSLATAVFDAGTRLA
                     NVIGLPIIALVISVWGWREAFWVQGILSIIYLAVFWRMYREPKEKRDEGRMSEAEYRY
                     IVDGGGAASKNKNFNLEDFKYLLKQRKVWGLSLGLAGVGYVLWMLFTWLPGFLQMGFD
                     QSVLKSGLFTAIPGIAMFVAEVTIGGVYVDHLIRKGHDASLVRKTVLVAGIIIAFLTV
                     GAGTATSPVSAITWIALGSVGIALVYVVSNSIPALIAPEGDTGSVAAIMNFVNLLAGM
                     ISPIVTGAIVDATGNFKYAFLVGGIALIGGLLSFVFLMGRVEPIPSRSEAEARRHPGH
                     MNLRVESRVEE"
     gene            6957..7658
                     /locus_tag="VFR20_06455"
     CDS             6957..7658
                     /locus_tag="VFR20_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0230193.1"
                     /translation="MGEARISDMLLTDQAFLAIKALLFDGEVHAGQLVSMAELVKRSG
                     LPLAPVREAVKRAEAVGLVEILPKRGVQIMAATPDTIQACFHLRCLIDQEGARVLASN
                     ITKDAIEALRDEHIAVREGAVKAVTPDIQRLAKKVDWELHSTLAGALNNNLAAQIYDS
                     NRDRITVLQQSRHFLPERIVPAMTEHLRIIDAILTGQTQEAMTAVRQHMVGTLRWWGV
                     APPEGNVPTSMRTTT"
     gene            7789..8844
                     /gene="alc"
                     /locus_tag="VFR20_06460"
     CDS             7789..8844
                     /gene="alc"
                     /locus_tag="VFR20_06460"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HEU0230194.1"
                     /translation="MASTNLPLGTIIDAPAVDELPHYIKQYPNLASQHLGTRVLSCTD
                     EFFADAQRMLQDAEPVFIVGKFDEHGKWMDGWETRRRRSGGNDHAIIKLGLPGVIRGL
                     DINTSHFTGNFPPAASIQAASCGGDPDSQTEWVEIVPVRSLHGNAHHFVEVNDARVWT
                     HLRLNIFPDGGIARLRVHGQPACDWGQRDPAGLHEVSALVNGGRIVACNDAHFGSPAN
                     VIKAGRGQDMGDGWETRRRREPGNDWLILALGHPAIIEKIEIDTAHFKGNYPDRVSVQ
                     GALVLESTDQSLITQAMFWPVILAEQKTEMDKQHFYASQDIIPLGPVSHVRVNIFPDG
                     GVSRVRIWGRLAARGAE"
     gene            8841..9395
                     /locus_tag="VFR20_06465"
     CDS             8841..9395
                     /locus_tag="VFR20_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HEU0230195.1"
                     /translation="MITETMPLLAVEPLTRATFALFGDVIEADDTMTRYSINSGSTER
                     YHDLARIDAGADGRAIVSIFRSQPRELPFVIAAMERHPKSSQAFYPLSGLPYLAVVAR
                     KSIRPALGDLRVFYCEGHQGVNYAPGIWHHPLLALLAPSDFLIIDRAGPGENCDIVQM
                     DIHSVIPQLPRLNTGHTRTRAPQA"
     gene            complement(9402..11030)
                     /locus_tag="VFR20_06470"
     CDS             complement(9402..11030)
                     /locus_tag="VFR20_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU0230196.1"
                     /translation="MSNFRISTRLAMSFCFMLLLIVVLAGVGIWRMQAASSIVRNIIQ
                     VRLKTERLITEWEKLTALNALRTTAASKADNAATAAYFKEHMGTTSARISELQKAVDA
                     AITDPGARALFNTVRQKRADYRDARSTALKAKASGNAQAAKQFFEKDMASYLSAYVGS
                     VGNLLAYQKRLIDTNVSQLHADNRTGMLLVAGVAALAILAGIVFAWLITRSIVGPLRR
                     AVGLAQTVSSRDLTAVIKVGGRDETGQLLRALKQMNDNLTGVVQEVRGGAEAIASASS
                     QIAAGNTDLSSRTEEQASSLAETAATMEELTTTVRQNADNAQQASHLAEAAADVAVKG
                     GDVVSRVVQSMGAIDESAKKVADIISVIDSIAFQTNILALNAAVEAARAGEQGRGFAV
                     VATEVRALAQRSASAAKEIKGLIDLSASATAEGNTLAAEAGAAMAETVSSIQRVTDIV
                     GEISAASREQTIGIEQVNVALSQMDQVTQQNAALVEEASAASDSLREQAASLADLVAT
                     FRLAAVERPRQALVEAQVPGIPRLPVGTVAALPA"
     gene            complement(11146..11538)
                     /locus_tag="VFR20_06475"
     CDS             complement(11146..11538)
                     /locus_tag="VFR20_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008347888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEU0230197.1"
                     /translation="MPFARISLARGKSREYLRALSDELHAALVESFEVPADDKFQVIH
                     QHDPEDMIFDPHYMGVDRSPDLVYITLTTGKPRSTETKKRFYQTLVRRLAARPGIRSD
                     DVMVVISTSQRDEWSFGGGLAQMVEDAS"
     gene            11642..12523
                     /locus_tag="VFR20_06480"
     CDS             11642..12523
                     /locus_tag="VFR20_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0230198.1"
                     /translation="MAAHNFDINALRTFETGVALGTFAKASDRVGRSPAAVSAQLRKL
                     EQQAGTELFRKAGRGLALTEAGETLLSYARQILAANDAALTALHSTQARGKVSLGLQE
                     DLGEWVLTDVLGHFARAHPGMRIETRVCRNTELMDRVAAGELDLAVVWGNGGAAPWRE
                     TLARLPMVWIGGLNGMGDTNAPQQLELVAFDGACLFKTAALEALNQAGIAWDVAFTSS
                     SLNGLLAAVAAGLGVTIRTPIGLPPRVAVLQPSASGLPGLPKVDLTMIATRSEQSPAV
                     AFLADALRTAIHDHLPH"
BASE COUNT         2464 a         4179 c         3824 g         2244 t
ORIGIN      
        1 ggcagattcg tgacggcgcg ttgcaccagg gagacaaact tcccaccgag tccgagctta
       61 tgcagttgca aggggtcagc cgcacggtcg tgcgcgaggc catttcgcgc ttgcaagcgg
      121 gaggcgtggt cgaaacccgg cacggcatcg ggtcgttcgt gttggggctg cccaatgagc
      181 agagttttca catcaacccc accgacctgg tcaccatcca ggacgtcctc aacgtgctgc
      241 agttgcgcca gagcctggaa tccgccgccg ccggcatcgc cgcccaacag cggtcgcaag
      301 acgacctcga cacgctcgcg gccatcctga cggaattccg ccagtgcctc gatgccggcc
      361 aagacaccgt ggacgtggac ttccgcttcc acctgtccat cgcccgcgcc accggtaacc
      421 gctatttcgt cgagttcatg tcctacctgg gccagaccgt cataccgcgc acccgtatca
      481 acacggcctc gctgggcaag gaaacccagc agcgctatct cgagcgcgtg cactacgaac
      541 acgaagaaat ctacaaagcc attctgctgc aaaaccccct ggtcgccagc agcgccatgc
      601 aaatgcacct gaacaacagc gcggagcgcc tgcgctccac cttgaaccac tgatacattc
      661 ctgataacgc acagctggca acggaagtgg agacaagcct gcctttatgt tgtacgataa
      721 ctgataaatt atcactacca ataatgaccc gcccgccgca cagcgccggc cggccaccca
      781 caatcccatc atctggaaca ggagaaactt gatggatccc cagaagctca agacggtgct
      841 cggctccggc ctgctgtcat tccctctgac agacttcgac acaaccggtg atttcaacga
      901 agctggctac cgtgcccgcc ttgaatggct gataccctac ggcgccaccg cgctgtttgc
      961 cgccggcggg accggcgaat acttctcgct gagcggcgac gaatacccgg ccatcatcaa
     1021 ggcggccgtc gacacctgcc gcgggcacgt ccccatcatt gccggcgcgg gcgggcccac
     1081 gcgcttcgcc atcgaatgcg cccgggcggc ggaacgggcg ggggccgacg gcatcctgct
     1141 gatgccgccc tatctgaccg aaaccagcca ggccgggctg gccgcgcata tcgaggcggt
     1201 gtgccgcagc gtgggcatag gcgtgatcgt gtaccaccgc gccaactcgc tgccgacccc
     1261 ggacacgctg gaacaactgg ccgggcgctg ccccaacctg atcggcttca aggacggcgt
     1321 gggcgacatc gagaaaatgt ccgccgtgta cacacgcatg ggcgaccgcc tggcctacct
     1381 gggcggattg cccaccgccg aggtctatgc cgccgcctac aaggcgctgg gcacccccgt
     1441 gtattcgtcg gcggtgttca atttcatccc caggacggcc atggaattct accgggccgt
     1501 cgaatccgac gacctcgcca cgcagcacca gttgttgaag acgtttttcc atccctacct
     1561 ggacatccgc aaccggggac ctggctacgc ggtcagcatc gtcaaggccg gcgcggctct
     1621 ggtcggccac gacgccgggc cggtgcgctc gccgctgtcg gacctgacac ccaaagaaag
     1681 caggcagctc ggcgagctga tcgagcgcct ggggccgcaa tgacaggaaa cgggtagccc
     1741 cgcgctggcc agggtgcggc acaccctggt ccggcccgct gcggcgccag gggttctctg
     1801 aacaagtcac cgcgcctgct cgccgctcga ctcgttcata ggcccctagt gtcctgtttg
     1861 actaattcgg ctacgtatct tcggcgcctg gactggccat gcggcgttgc aaatcctcgc
     1921 gatagccacg gctatcgctg cggtttgcgc cttgcccggc acgcctatgt attcgataca
     1981 agtgtgcgaa ccaatcaagc aggacactag gcgctttccc gcacgatcag ctcgaagccc
     2041 agatcgatcg ccggcgattc gatcggttcc ttgtcgatca tggacaacaa caggcgcgcc
     2101 gcggtgcgcc ccatctcggc gcgcggggtg cgcacggtgg tcaggcgggg aacgcaatcg
     2161 gccgcccccg cgaggtcatt gaacccggcg atggcgattt ggccgggcac gtcgatgccc
     2221 aggcgctgcg cctcgaacag cgcgccttgc gccagatcat cgttcccgaa aaatatgccg
     2281 tcgacgtccg gacgacgctc gagcaattgg cgcaacagct cacaccccag gccgatgctg
     2341 gacggcgccg tcgtgagaac ctcgaggccc gggtcatgca gaccgccggc acgcaggaca
     2401 tcgcggaacc cctcaccccg ctgcagcatg cgtggatcca gttgcgcggc gacaaaggca
     2461 ttgcggcggc agccacgcgc aatcaggtgc cgggcgacgg ccgcgcccgc ctgcgcctgc
     2521 gaaaaaccca cgcagtgcac accgaccgca ttcgtcagct ccatgatgtg cacgcaaggc
     2581 acaccgcttg catcgataag ccgtcgcgcg acttcgctgt ggtcgaaccc ggtgacgatc
     2641 atgccgccgg gcggcgaggc aaggtaatgc cggatcagat gctcttcggc ttcacgcgaa
     2701 tagtgcgaat tgccgatcag cagttccagg ccgcggggcg caagcacgcc gtggatggct
     2761 tcaagcgtgt cgagaaaaac ggtgttggac agcgaaggca ccaggaccgc cactgcggcg
     2821 ctgcacgccg aagcaagcgc acgcgcagcc ggattggcga cgtagttcag cgccagtgcg
     2881 gcctctttga cgcgctggac atattccgga ccgaccgtgc tcaggccacg cagggcacgt
     2941 gaggcggtga tggtgctgac accggcccgc tgtgccacct gcgccagggt gggcattccg
     3001 gtactgcggg ttttcctgaa tgtcgatgtg gccatggtca gcgtaggata gcgctgtctt
     3061 agtagtgacg caatgcttgc tatcccgtag acgatccgtg gcggaagctc cctgaggcgg
     3121 cctggtgtgg ccgccatgtt ccagccgcga aagatagcgc tatcttaata tgaaatcaac
     3181 ctcgacagac ggaaatatca tggccttggt ggtgatgggc gtggcgggct gcggaaagac
     3241 cagcgtcgcg caagcgatcg tggcaagaat gggcggatgc ctgatcgaag gcgatacatt
     3301 ccaccccgcc gccagcgtcg agaaaatgcg acgtggcatc gcgctcaccg acgaggacag
     3361 aggcggctgg ctggaccgac tcgccacgga actgagggac accgcccggc gcgacgaacg
     3421 cgccgtactg gcgtgttccg cgctcaagcg gcgctatcgg gaccaattgc gccgcggcac
     3481 tccgacactg ggcttcgttt tcctgcgcct gtcccagcgc gaggccctga cacgggtgac
     3541 gcagcgcatt ggccattaca tgccggcatc cctggtggcc agtcagttcg ccgacctgga
     3601 gcccccggtg tccgagccgc tgtcgttgag tgtggatgcc acgcttccgg tcggcgatat
     3661 cgtcgaccag gtagcccgct ggtatgccca gacggtgaca caagagggat tccgcgccgt
     3721 gacaaacgtg acgcactgac tgtcagtggc acgacgggag tcacgcgctc gaaagagata
     3781 gcgctgtctt tacagcaaac cccaggagca aaagaataca ggtttgccgg caccgcaagc
     3841 ccggcactgc ggaacaaatt ttcaggaggt ggtaaatggc aacagtggca agacaaacaa
     3901 tcgcaagaca aaaaacaccg aatcgacgct ggatgatagg tgtactgctg ggtatcggtg
     3961 tgctcatcaa ttatttcgac cgcatcaatc tttcggtcgc cggcccgcaa ctgacgcagg
     4021 cattccactt gacgccggcg gagctgggct tgttgttcag tgctttcttc tggccgtacg
     4081 cattgctgca gatccccgca ggcctgctgc tcgaccgcta cggggtcacc cgcatcggac
     4141 gctggggcgc gttcctgtgg gggttggcct cgggaatgat ggtgctggtc agcggcttcg
     4201 gcggcgtttt cgccgcgcgg gcgttgctgg gcgtcgcgga ggcgcccggg ttcccggtca
     4261 gttcaaaagc caccggatac tggtttccgc gcaaggaacg ggcgctcgcg accgcgatct
     4321 tcgatgcggc cgccaagttc tccaacgtca tcggcgtgcc gctggtggcg ctcgtcgtcg
     4381 tgaagctggg ctggcgctgg gggttcggcg tgaccgccac cctgagcctt ttatatttcc
     4441 tggctttcta tttcatctac caagacccca gcaaggatac ccgactgagc caggaagaac
     4501 gccactacat cgtgcagggc ggcgccacgc ctgaagggcc ggcgtccact ggttccatca
     4561 acatgctggg ctatttgttg acgaaagcca aggtctgggg gttgaccatc ggcttcgccg
     4621 cctacggcta ttcgttctac ctgttcctga cctggctgcc cgaatacctg gtgcagacca
     4681 tgcacatgag catcctcaag tccgcggaat tcgccgcgat cccttgggcg gtggcaacgg
     4741 tcaccgatct tgtcgtgggg ggctggctga tcgaccacct gatcgcgcgc ggccggaacg
     4801 agacgcgagt gcgcaaagcg gtgctggtta tcggaatgtt gctcggcctg gcagtattcg
     4861 gcgccacccg cacggtcaca ccaggctggg cgattttctg gatctccgtc gcgctgggcg
     4921 gcctggccac cgccgctccg gtgggctggt cactgccttc gctgatcgcc ccccggggcg
     4981 gtgtcggcac cgtcggcggc atcatgaact tcgtcaacaa cctgatgggc gtggcggcgc
     5041 ctgtcgtgac cggcttcatc gtgggggcaa cgcattcctt caccaacgcc ttcacggttg
     5101 ccggcgtggt cctggcgatc ggcatcgtgt cattcgtatt cgtgatggga cgcatagagg
     5161 ccattcccga atacagccaa gcgggggatc gcaagcgcga accacttgcc acgggtggtg
     5221 cgggacaagc ggaataagcc gcacggggcg aggcatccga tgcctcaccc ccgcttgttt
     5281 cttcgagact gcgtgccccg gcggggcgta ctctttattc ctccacccgg ctttccacac
     5341 gcagattcat gtgccccggg tgcctgcggg cctcggcctc gctgcgactc gggatgggct
     5401 cgacccgccc catcaggaac acgaaactca gcagaccacc gatcaaggcg atgccgccca
     5461 ccaggaacgc atacttgaag ttgcccgtgg catcgacgat agcgccggtg acgatgggcg
     5521 aaatcatgcc ggcaagcaga ttgacgaaat tcatgatggc cgccaccgag ccggtgtcac
     5581 cttccggcgc gatgagagcc ggaatggaat tgctcacgac gtaaaccaac gcaataccca
     5641 cactacccaa tgcaatccag gtgatggcgg aaacaggact ggtcgcggtt cccgccccca
     5701 cggtcaggaa cgcgatgatg atcccggcga ccaggaccgt cttgcgtacc aggcttgcgt
     5761 catgcccctt ccggatcagg tggtccacat acacgccgcc gatggtgact tctgccacaa
     5821 acatggcgat gccgggaatg gcggtgaaca agccgctttt gagcacactc tggtcaaagc
     5881 ccatctgcag gaatccgggc agccacgtaa agagcatcca caacacgtac cccacacccg
     5941 cgagccccag cgacagcccc cagaccttac gctgcttgag caggtatttg aaatcctcca
     6001 gattgaaatt cttgttcttc gaagccgcgc cgccgccgtc gacgatgtag cggtattcgg
     6061 cctcgctcat gcggccctcg tcacgttttt ccttgggctc gcgatacatg cgccagaata
     6121 cggccaggta aatgatgctg agaatgcctt gcacccagaa cgcttcacgc cagccccaaa
     6181 ccgagatgac cagtgcgatg atgggcagcc cgatcacgtt ggctaggcgg gtgccggcgt
     6241 caaacaccgc tgttgccagg ctgcgttcgg tcaccgggaa ccaggtcccg gtggccttca
     6301 tggcccccgg gaaggcgggc gcctcggcca ggccaagcaa taaccgcgcc agaatgacga
     6361 cccagtagcc cgaggccacc gctgtcagga agctggcgat cgcccacgtg gccatgccga
     6421 ttcgccaaat ccacttcgcc cccatgcggt cgagcaggat gccgctggga atctgcatca
     6481 aggcataaga ccacacgaaa gccgacccaa tgatcccgaa atcggtagcc gtcagcccga
     6541 aatcgtgaga catcggcgtc gccacaagtg ccaggctgag ccggtcgata tagttgatga
     6601 gaatgccgac cccgagaagc acggcgatgc tccatcgcac cttcccaata gccaaactgg
     6661 cgtccttgtg ccccgtagag ccactatttt gcttagacat gaacggtttc tccgaaatga
     6721 taagtctcca gcacctcaag acaatcacac cgccggaatc gccatggttc cgttgaagga
     6781 acgctccggt acggaacccg ggatcggatg atgcatcttg actgctatcc acaagcccga
     6841 taacatttat caaaacatgt tagataacat ttgtggaata atatatgtgg agacacgacc
     6901 aaacaatggt gattacccta gattcagtcc agccgcacgg gaaggagaat aaagaaatgg
     6961 gtgaagcacg aatatcggac atgttgctga cggaccaggc gtttctggcc atcaaggcgc
     7021 tcctcttcga cggggaagtc catgctggcc aattggtgtc catggccgaa ctggtcaagc
     7081 gctcgggctt gccgctcgcc ccggtgcgcg aagccgtcaa acgcgccgaa gctgtggggc
     7141 tggtggaaat cctgcccaag cgcggcgtgc agatcatggc cgccacgccg gatacgatcc
     7201 aggcatgctt tcatctgcgc tgcctgatcg atcaagaagg cgcccgcgtg ctggcatcga
     7261 acatcaccaa agacgccatt gaggcgctgc gcgatgaaca catcgcggtg cgcgagggcg
     7321 cggtgaaagc cgtcacaccc gacatccagc ggttggcaaa gaaggtcgac tgggagttgc
     7381 attcgacact tgcaggcgcg ctgaacaaca atcttgccgc ccagatctac gacagcaatc
     7441 gcgaccggat cacggtcctg cagcaatcac ggcacttcct gcccgagcgc atcgtgcccg
     7501 ccatgaccga gcatctgcgc atcatcgatg ccattctcac cggccagacc caagaggcca
     7561 tgacagccgt gcggcaacac atggtcggca cgctgcggtg gtggggcgtc gcgccgccgg
     7621 aaggcaacgt gcccacgtcc atgcgaacga ccacttagcc gaacccgcgc actctcacac
     7681 ccatccgtgc cagaacatgc aacggcgcat gcacgttgca aggcaccggt gtttcctgcc
     7741 gcctcatgcg cagaaacatt ttcctcaaca aacaggagac aacttcttat ggccagcacc
     7801 aacctgcccc tcggcacgat catcgacgcc ccggccgtcg acgaactacc ccactacatc
     7861 aagcagtatc cgaatcttgc gagccagcac ctgggcaccc gcgtcttgtc gtgcacggac
     7921 gaattcttcg cggacgcaca gcgcatgctg caggatgccg agcccgtatt catcgtcggc
     7981 aaattcgacg aacatgggaa atggatggat ggctgggaga cgcgccggcg gcgcagcggc
     8041 ggcaacgatc acgccatcat caaactcggg ttgccgggtg tcatcagggg gctggacatc
     8101 aacacctccc acttcaccgg caacttcccg ccggcagcct cgattcaagc cgcatcgtgc
     8161 ggcggcgacc cggacagcca gaccgaatgg gtggaaatcg tgcccgtccg gtcattgcac
     8221 ggaaacgccc accatttcgt tgaggtcaac gacgcacgcg tgtggacgca cctgcgcctg
     8281 aacatctttc ccgatggcgg cattgcccgc ctgcgcgtcc acgggcaacc ggcctgcgac
     8341 tgggggcagc gcgaccccgc cggcctgcac gaggtgtccg ccctcgtcaa cggcgggcgc
     8401 atcgttgcct gcaacgatgc ccactttggc tctcccgcca acgtgatcaa ggccgggcgc
     8461 ggccaggaca tgggcgacgg ctgggaaacc cgccgccgcc gggaacccgg caacgattgg
     8521 ctcatcctgg cgcttggcca cccggccatc atcgagaaaa tcgaaatcga caccgcgcat
     8581 ttcaaaggca attaccccga ccgggtctcc gtccagggcg cgctcgtgct ggaatcgacc
     8641 gaccagtcgc tgatcaccca ggccatgttc tggcccgtga ttctggccga gcagaaaacg
     8701 gagatggaca agcagcattt ctatgcaagc caggacatca tccccttggg cccggtatcg
     8761 cacgtgcgcg tcaacatctt cccggacggg ggcgtgagcc gggtgcgcat ttgggggcgc
     8821 ctggccgcgc gaggcgcaga atgatcaccg agaccatgcc gctgctcgcg gtggagcctc
     8881 tgacgcgcgc cacgttcgcg ctcttcggcg acgtcatcga ggctgacgac acgatgaccc
     8941 gctattcgat caatagcggc agcacggaac gctatcacga cctggcccgc atcgacgctg
     9001 gcgcggatgg ccgtgcgatt gtgtcgatct ttcgcagtca accccgcgag ttgccgtttg
     9061 tcattgccgc gatggaacgc catcccaaat cgagccaggc gttttacccc ttgtccggcc
     9121 tgccgtacct tgcggtcgtg gcccgaaagt cgattcggcc cgcccttggc gacttgcggg
     9181 tgttctattg cgagggacac caaggggtga actatgcccc cggcatctgg caccacccgc
     9241 tgctggccct gctcgccccg tcggactttt tgatcatcga tcgcgcaggg ccaggcgaga
     9301 actgcgacat cgtgcagatg gacattcaca gcgtgattcc ccaacttccg cgcttgaata
     9361 ccgggcacac gcgcactcgc gcaccccagg cctgactcag gctacgcggg cagcgccgcc
     9421 accgtcccaa ccggaaggcg ggggatcccg ggcacctggg cttcaacaag ggcctggcgc
     9481 ggccgttcca ccgctgccag cctgaacgtg gccaccaggt cggccaggct ggccgcttgc
     9541 tcgcgcaagc tgtcggacgc ggccgacgcc tcttccacca gcgccgcgtt ctgttgcgtc
     9601 acctgatcca tctgcgatag cgccacgttg acctgctcga taccgatggt ctgttcgcgg
     9661 ctggcggcgg agatctcgcc cacgatatcg gtgacgcgct gaatactgga caccgtttcg
     9721 gccatggcgg cgcctgcctc ggcggccagc gtgttgcctt cggccgttgc cgacgcggac
     9781 aaatcgatga gccccttgat ctctttggcc gccgacgcgc tgcgttgtgc gagcgcccgc
     9841 acctcggtgg ccaccaccgc gaaaccgcgc ccctgctcgc cggcgcgagc ggcctccacc
     9901 gccgcgttca aagccaggat attcgtctgg aacgcgatgc tgtcgatcac gctgatgatg
     9961 tccgcgactt tcttggcgga ttcatcgatg gcgcccatgg attgcaccac ccgcgacacc
    10021 acgtccccgc ccttcaccgc cacgtcggcg gcggcttcgg ccaagtgact ggcctgctgg
    10081 gcgttgtcag cgttctgccg caccgtggtc gtgagctctt ccatggtggc ggcggtttcg
    10141 gccagcgaac tggcctgctc ttcggtgcga gaggaaaggt cggtattgcc ggcggcgatc
    10201 tggctggagg cgctggcgat ggcctcggcc ccgccgcgca cctcctgcac cacgccagtg
    10261 aggttatcgt tcatttgctt taaagcccgc agcaattgcc cggtttcatc gcggccaccc
    10321 accttgatga cggcggtgag atcgcgcgag gacaccgttt gcgccagccc cacggcgcgc
    10381 cgcaacggcc ccacgatgga gcgagtgatg agccacgcga acacgatgcc ggcaaggatc
    10441 gccagcgccg ccacaccagc aaccagcaac attcctgtgc gattgtcagc atgcaactgg
    10501 ctgacattgg tatcgatcag gcgcttctga taggcaagca gattgcccac gctcccaaca
    10561 taagcgctca gataggacgc catgtccttt tcgaaaaact gtttcgcggc ctgcgcgttg
    10621 ccgctggcct tggccttcag tgccgtgctc cgcgcatccc ggtagtcggc gcgcttctgc
    10681 cgcacggtgt tgaacaacgc ccgagcgccc gggtcggtga tggctgcgtc caccgccttt
    10741 tgcaattcgg aaatgcgggc actggtggtg cccatgtgtt ccttgaaata cgccgccgtc
    10801 gccgcgttgt cggccttgga ggcggccgtg gtgcgcaatg cgttcagcgc tgtcagcttt
    10861 tcccactcgg tgatcaggcg ctcagtcttc aagcgtacct gaatgatgtt ccgcacgatg
    10921 gagctggcgg cctgcatgcg ccagattccc acacccgcca aaaccacaat gagcagcagc
    10981 atgaaacaaa agctcatggc caggcgggtg ctgattcgga agttgctcat gattcgtcct
    11041 atgcgcgatg gagtgatcgg cacaagccgg ccatcccaac agccttaacg tctggagcgg
    11101 acaaacttta agagcctggt aacaatatcg ccgaactgtc cgtgttcatg atgcgtcctc
    11161 caccatttga gccaggcctc cgccaaacga ccactcgtcg cgctgcgaag tcgagatcac
    11221 caccatgaca tcgtccgacc ggatacccgg ccgggcggcc agacggcgca ccagtgtctg
    11281 gtagaaccgc ttcttggttt ccgtcgagcg gggcttgccg gtcgtcaggg tgatgtacac
    11341 caggtcggga ctgcgatcca cccccatgta gtggggatcg aaaatcatgt cttccggatc
    11401 atgctgatga atgacctgga atttgtcgtc ggccggaacc tcgaaggatt caaccagcgc
    11461 ggcgtgcagt tcgtcactga gggcccgcaa atactcgcgg gacttgccgc gtgccaggga
    11521 aatgcgtgcg aaaggcatga tggactcctg aaaaaacgat tgatgcagga atcttaagtc
    11581 gctatattgt tctgataaat caaattgttt aacactaaca attaaattta atggaattaa
    11641 tatggccgcg cacaacttcg acatcaacgc cttgcgcacc ttcgaaaccg gcgtagcgct
    11701 gggaacgttc gccaaagcct ccgatcgcgt cggccggtcg ccggcggcgg tcagcgccca
    11761 gctccgcaag ctggaacagc aggcggggac ggaactcttc cgcaaggcgg ggcgcgggct
    11821 ggcgctcacc gaagcgggtg agaccttgct cagttacgcg cgccagatcc tggcggccaa
    11881 cgatgccgcg ctcaccgcgc tgcacagcac ccaggcgcgc ggcaaggtca gcctggggct
    11941 gcaggaagac ctgggggaat gggtgctcac cgacgtcctg ggccatttcg cgcgcgccca
    12001 cccgggcatg cgcatcgaaa cccgggtgtg tcgcaacacc gaattgatgg accgtgtcgc
    12061 cgccggcgaa ctcgatctcg ccgtggtctg gggcaatggc ggcgccgccc cgtggcgcga
    12121 aacactggcg cgcctgccga tggtgtggat aggcggcttg aacggcatgg gcgacaccaa
    12181 cgccccccaa cagctggaac tggtggcgtt cgacggcgcc tgcctgttca agaccgccgc
    12241 cctggaagcc ttgaaccagg ccggcatcgc ctgggacgtg gccttcacca gctccagcct
    12301 gaacggtctg ctggccgctg tggcggcggg cctgggggtg accatacgca cacccatcgg
    12361 gttgccgccc cgcgtggccg tgctgcagcc gtcggccagc ggcttgcccg gcttgccgaa
    12421 agtggatctc accatgatcg cgacccggtc tgagcagtca cccgccgtgg cgttcctggc
    12481 ggacgccctg cgcacagcca tccacgacca tctaccccac tgaaggcgcc aagcgcggcc
    12541 tcgtcctcgg aaaccacttc tccggagtcg cgccaatcac cacggcgcgc acgtcaagcg
    12601 cttctgcgta aagcgcgcca aacgcctacg tgaacagaat cgagatattc agaatcaggc
    12661 acaccgccca gatcaccgac cgcaccgttc cccgcccgcc tatgtacacc c
//
LOCUS       DASTNK010000083        24371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_244378, whole genome shotgun sequence.
ACCESSION   DASTNK010000083 DASTNK010000000
VERSION     DASTNK010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 24371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24371
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244378"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..246)
                     /locus_tag="VFR20_06485"
     CDS             complement(7..246)
                     /locus_tag="VFR20_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEU0230199.1"
                     /translation="MSDSTAPSGDTQKLTGTVKWFNDAKGFGFITPDNGGEDLFAHFS
                     SIQMNGFKTLKEGQKVAFEIAQGPKGKQALNITAA"
     gene            441..764
                     /gene="clpS"
                     /locus_tag="VFR20_06490"
     CDS             441..764
                     /gene="clpS"
                     /locus_tag="VFR20_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEU0230200.1"
                     /translation="MATQTERQREVVVERQEERLAPPPMYQVVLLNDDYTPMEFVVDV
                     LRRIFGKSEDEASRIMLKVHHEGRGVCGVFPREVAETRVEMVRRSAQERQHPLQCIME
                     PAPAD"
     gene            906..3218
                     /gene="clpA"
                     /locus_tag="VFR20_06495"
     CDS             906..3218
                     /gene="clpA"
                     /locus_tag="VFR20_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEU0230201.1"
                     /translation="MISEELEVSLHMAFVEARTARHEFITVEHLLLSLLDNVAAVEVL
                     RACSANIEALRRDLRKFINENTPVFPGEGEVDTQPTLGFQRVIQRAIMHVSSGNSTKK
                     SVTGANVLVAMYGEKDSHAVYYLLQQGVTRLDVVNFLSHGITKASDDSSPEPVKQPGA
                     ETEHKPEQHKSPLELYALDLNFESREGRIDPLIGREAEVERVIQVLCRRRKNNPLLVG
                     EAGVGKTAIAEGLAWRIVKGDVPDILADAQVYALDMGALLAGTKYRGDFEQRLKSVLR
                     SLKDNANAILFIDEIHTLIGAGSASGGTLDASNLLKPALSSGQLKCLGATTYNEYRGI
                     FEKDHALSRRFQKIDVVEPTVEQTIQILRGLKRYFEAHHSVRYSAAALTAAAELSARH
                     INDRYLPDKAIDVIDEAGAAQRLLPRSRQKKLIGKAEVEATVAKIARIPPQSVSNDDR
                     SKLATLERDLKTVVFGQDAAIEALAGAIKMARSGLGRPDKPIGAFLFSGPTGVGKTEV
                     ARQLAFTMGIELLRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEAISKQPHCVLL
                     LDEIEKAHPDVYNILLQVMDHGTLTDNNGRKADFRNVIIVMTTNAGAEALNRNTIGFA
                     TPETAGDELTDIKRLFTPEFRNRLDAIISFKPLSREIILRVVDKFLMQLEDQLHEKHV
                     DVVFSQALRGHLAKEGFDPAMGARPMQRLIQDTIRRALADELLFGRLVDGGHVEVVLD
                     EHDKVTLVFLPTDGKPKHLPPVDSEAELVH"
     gene            3337..4518
                     /locus_tag="VFR20_06500"
     CDS             3337..4518
                     /locus_tag="VFR20_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEU0230202.1"
                     /translation="MWSVSQWLALACTALIVFAIANYFPIVTLRLKWLIANASLPQAL
                     MLTWEQGRPVVAVMTGLFGFLVPLTQLLFLVWALMCVASGNLPRDFAMGMRVLRVLGT
                     WSMVPVLMLGILVAIVKLAGLAVLHVAPGLWGFVALTVLLTLLERVSAGWLWRQAEDA
                     GLVPLSGIDVDPAATVASCHACGNVQNLASPHDHQACRRCGAKVRFRLPGMSRTWALV
                     LAACILYVPANVLPVMKFRTPVGTTEHTILGGVVELWKLGSWDLAIIVFVASIVVPMT
                     KLLILIALMARRCWLGDDIQRQRTRLYELVEFIGQWSMLDVFVVILLTSMANFPGISQ
                     ISAGPGAASFGLVVVLTMLASAGFDPRHGWDARPSLGSSDGRKTAVGGERLAGPATEH
                     A"
     gene            4515..6185
                     /locus_tag="VFR20_06505"
     CDS             4515..6185
                     /locus_tag="VFR20_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEU0230203.1"
                     /translation="MTEPTPSEPAASTDVPATAGTPAAPHVVAPKRRSVSWIWLVPLL
                     VAVVGLSLIVKNWMETGPIVTISFQSANGIEVGQTTVRYRDVVVGVVNDIEVSPDRSR
                     VLVRVRLKHDAAAYIARKGTRFWVVRPRLELTGVSGLNTLLSGAYIGVDTSRTKDHGV
                     ETAEAYRFIGLEKPPEITSDKPGTRYTLTAPDLGSLELGSPVYYRRIQVGRVIDYGLA
                     PDGKLVDIQIFVDAPNDKYVTADTRFWNVSGIQLSVGSDGVTVQAGSLASVISGGVEF
                     ASADAAITTPAKANTTFSLQSTRQAAMADPDGIPVPINMEFRQSVRGLSVGAPVMFHG
                     LEIGKVTDINLEYNVKIRRFYVLVKTELFPMRFGKVYEDLIAENEKSGNAGHNLLRPM
                     IEHGLRAQLRTGNLLTGQQYIALAFVPDASPVHFDETQKPPLIPTVPGSFDRLQEQVS
                     SLVTKLDAIPFAGISQDLRGALKALTRTLNGLHGVGPQTETTLKAVRKSLDQVSGLLG
                     SAASGGNLGQTLRELGQAAKSLRALADYLQANPSALLRGRPADVLPGH"
     gene            6207..6854
                     /locus_tag="VFR20_06510"
     CDS             6207..6854
                     /locus_tag="VFR20_06510"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HEU0230204.1"
                     /translation="MKYSYLIPTLVLIALLGGCAAAPKIQYYTLIPPLADTALANAAS
                     KATAGRYAISVQPVSVPDQVDRPQIVISGGDSARVTPLNESLWVSPLGDQVRRALSAD
                     LSARLGTLDIAADTAPSSLPVWKIFLTVQRFDAIFGRQTILDATWRLDPVHLRGRRPT
                     ICRAEVTVPVGAGVPALVMGQRKALWTLSSLIAAQISGGPPAPSNRPGVVLKGCA"
     gene            7141..10956
                     /gene="putA"
                     /locus_tag="VFR20_06515"
     CDS             7141..10956
                     /gene="putA"
                     /locus_tag="VFR20_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HEU0230205.1"
                     /translation="MATTTLGVKVDEALRERLRHAAQKLGCTPHWLHKQALFAYIDAI
                     ERGQVPAEISYLNQQDVAEEAGEVAHQDPLPPFYEFAQDVQPQSVLRAAITSAYRRPE
                     TECVPLLADQARDIPGPDVHALAERLVKTLRSKRKGGGVEGLIQEFSLSSQEGVALMC
                     LAEALLRIPDRATRDALIRDKISRGDWRAHMGESPSLFVNAATWGLMLTGKLVSVNSE
                     QSLSRALTRLIGKGGEPLVRKGVNIAMRMMGEQFVAGQNISAALANSRKLEEKGFRYS
                     YDMLGEAATTAADADRYNTSYEQAIHAIGKSAQGRGIYEGPGISIKLSALHPRYSRAQ
                     RDRVMAELLPRVTALARLARSYDIGLNIDAEEADRLEISLDLLEALCFDPDLAGWNGI
                     GFVIQAYQKRAPFVIDYVIDLAKRSRHRLMVRLVKGAYWDSEIKRAQEGGLEGYPVYT
                     RKIYTDISYLACARKLLAVPDAVYPQFATHNAYTLASIYHLAGQNYYPGQYEFQCLHG
                     MGESLYEHVTGPVAQGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSFVNQIGDD
                     DIPVEALIIDPVQAAQRIQPLGAPHDKIPLPRDLYQARDGRSNSMGLDLSNEQRLGSL
                     SAALLGSATATWRAQPLLGGAQPEWDDSRAQPVRNPADRRDVVGHVIEASDAEVRQAL
                     QEAAHAAPIWQSTPVEERAQCLERAAQMLEDDMQPLLGLIVREAGKSLPNAISEVREA
                     TDFLRYYAGQIRSAFSGDTHRPLGPVVCISPWNFPLAIFTGQVSAALAAGNPVLAKPA
                     EQTCLIAAAAVAILRKAGVPSGAVQLLPGLGETVGASLVASPVVRGVMFTGSTEVAQI
                     ISRTLAQRLDDAGHPIPLIAETGGQNAMVVDSSALAEQVVFDVLTSAFDSAGQRCSAL
                     RVLCVQEDCADHVLGMLRGAMQELRVGNPDMLSTDVGPVIDMQAQAVIEKHIAAMRDA
                     GRRVDQLGLDSMCQHGSFVAPTIVELDDISALKREVFGPVLHVIRYKRDGLDALIDAI
                     NATGYGLTFGVHTRLDETVARVTSRIHAGNVYVNRNIVGAVVGVQPFGGEGLSGTGPK
                     AGGPLYLLRLLSRRPAGLPDWWQEPDAGGRELELPGPTGETNTYHLKPRGTVLGVAAS
                     REGAVAQYQACVTTGNALALAETDAARAFLADCTPEQKKRVLLVPPGDIGQADYQAVL
                     FEGDGDALRELNQVVAARSGPIVSVQGLSTDELAAGHQYSLERLLREVSVSVNTAAAG
                     GNASLMMVG"
     gene            complement(11052..11513)
                     /gene="dut"
                     /locus_tag="VFR20_06520"
     CDS             complement(11052..11513)
                     /gene="dut"
                     /locus_tag="VFR20_06520"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU0230206.1"
                     /translation="MFRRIETRIVNPLLGTRIPAPGYATDGSAAMDLRACLDEAVTVA
                     PGGRTLVPTGIALNMMDPGLVAIVASRSGLSLKHGVRVAQGIGVIDSDYHGEIAVILA
                     NDSDTPYRVEPGDRIAQLMFQPVVQVDLHVVDAFTIETERGAGGFGSTGKA"
     regulatory      11648..11825
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            11948..12442
                     /locus_tag="VFR20_06525"
     CDS             11948..12442
                     /locus_tag="VFR20_06525"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU0230207.1"
                     /translation="MSNPRSGCSGAVASARSIAFVQAGWHSDIIAQARLSFLDEITRS
                     GFDPRDIHVIDVPGAFEIPLHARLLAETGRYSAIVAAGLVADGGIYRHEFVAQAVVSG
                     LMQVQLETRVPVLSCVLTPQERFEGDDLHRFFFEHFRIKGVEAARACAATLSSLETLQ
                     LIGG"
     gene            12447..13148
                     /locus_tag="VFR20_06530"
     CDS             12447..13148
                     /locus_tag="VFR20_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase subunit alpha"
                     /protein_id="HEU0230208.1"
                     /translation="MFTGIVQGMATIDSIDDREGLRTFSLMFPPGFCDGLAVGASVSV
                     DGVCLTVTHLLSANAASFDVMLKSLDLTTLGGYVRGGRVNVERAARDGAEIGGHPLSG
                     HVDFHAPLVAVRQMDNNRVLRIGIPPGFRKYVFAKGYIAVNGASLTVADVNRDEGWFE
                     VWLIPETRRATVFEQKQTGDALNVEIDRGTQVIVDTVRETVLECLGGLQPVLEAALRD
                     KGLEIEDLLRLPPRA"
     gene            complement(13197..13508)
                     /locus_tag="VFR20_06535"
     CDS             complement(13197..13508)
                     /locus_tag="VFR20_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230209.1"
                     /translation="MIRHPLGNPASSARRDNAGQVRAVSMPRRLIALVLAVASVVLIV
                     DALLNGWRLFNEGGAWDDWAPAVIMLVAGVILLLGARAALNPEVTPELPETSSPDHIE
                     H"
     gene            complement(13528..14733)
                     /gene="coaBC"
                     /locus_tag="VFR20_06540"
     CDS             complement(13528..14733)
                     /gene="coaBC"
                     /locus_tag="VFR20_06540"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEU0230210.1"
                     /translation="MRELADKRIVLGLTGGIACYKTAEFLRRALDQGGTVDVVMTEAA
                     TRFITTTTMQALSGRPVYVDTFDAGPSDGMAHIHLTRGADAILVAPASTDFIARLANG
                     LANDLLSTLCAARGACPLLLAPAMNREMWLNPPTQRNIRQLAEDGVVILGPGSGSQAC
                     GEVGDGRMLEPLELLDELIGSFQPKDLRGRRIVITAGPTSETVDPVRVITNRSSGKTG
                     YALARAARQAGADVVLVSGPTALQAPYGVTRIDVDSARQMHAAVMEAVADADIFIAVA
                     AVADWHVVHPSTQKIKKRGQAAPFDLEFAPNPDILADVAARPNGPWCVGFAAETENLE
                     AYAQAKRTRKGVALMVGNLAQQSMGADDTQLVLFDDTGAHPLPRLPKPEAARQLMRAI
                     AARLPNNPR"
     gene            complement(14817..15341)
                     /gene="lspA"
                     /locus_tag="VFR20_06545"
     CDS             complement(14817..15341)
                     /gene="lspA"
                     /locus_tag="VFR20_06545"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU0230211.1"
                     /translation="MPDSTLRGVGARHRAKLWVWLAGALAIIVLDQVSKLYVNSHLQY
                     GERLHVLPVFDITLLYNPGAAFSFLATDGGWQRWLFTAIALAAAGLIIHLLRKNPGQT
                     LFCASITAILGGAVGNVIDRLQHGHVIDFFLFYWRDWYFPAFNVADVAITCGAALLIL
                     DELLRMRRDRNKAG"
     gene            complement(15334..18171)
                     /gene="ileS"
                     /locus_tag="VFR20_06550"
     CDS             complement(15334..18171)
                     /gene="ileS"
                     /locus_tag="VFR20_06550"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU0230212.1"
                     /translation="MDYRDTLNLPESPFPMRGNLAKREPGWITQWEENKVYASIRAAS
                     KGRPKFVLHDGPPYANGDIHIGHAVNKILKDIIVKSRTMAGYDAPYVPGWDCHGMPIE
                     IQIEKKYGKNLPVEEVQSRARAYALEQIDRQRQDFKRLGVLGDWDHPYLTMNHSNEAD
                     ELRVLARIMEKGYVYRGLKPVNWCFDCGSALAEAEVEYADRTDPAVYVALRFHDKEKL
                     AHAFGLPQIDDGAAVIWTTTPWTLPANQALNIGADIDYSLVRLAQPRATGSMLLLATD
                     LVESCLQAWELEGRIVATVKGAALANLEFRHPLHDLDPGYRRLSPVYLGDYVTLDAGT
                     GIVHSSPAYGVEDFISCKAHGLEDDQIVNPVMGDGHYNPSLPLFGGLMIWKANPKIVE
                     ALQNSGALLKVEQHKHSYMHCWRHKTPIIYRATSQWFAGLDLQPKSGASLRETALQGI
                     EQTEFFPSWGRARLYAMIANRPDWTLSRQRQWGVPMAFFVHRETGALHPRTVELLEEV
                     ARRVEKQGIEAWQTLDPRELLGDEADQYEKNRDTLDVWFDSGSTHATVLGGRDHQLKG
                     SHADSLNWPADLYLEGSDQHRGWFHSSLLTGCMLYGRPPYKALLTHGFVVDGKGRKMS
                     KSVGNVVAPQKVSDTLGAEILRLWTASTDYSGELTISDEILKRVVESYRRIRNTLKFL
                     LGNLLDFDARANALPLEKMFEIDRYALALTAQMQSEVLASYNRYEFHLAVSRLQIFCS
                     EDLGAFYLDILKDRLYTTGADSVARRSAQTALFHVTHALLKLMAPILSFTAEEAWSDM
                     HASQETQTIFTELFHAIPAQPDAGLLTDKWTRLRAIRADAMRKIEEVRGTGAIGSSLD
                     AEVDIHADGDDHKLLDSLGDDLRFVLIVSRASLHEASGPITIDVAATTQPKCGRCWHH
                     RADVGTDAAHPDLCGRCTDNLFGTGEARQHA"
     gene            complement(18161..19126)
                     /locus_tag="VFR20_06555"
     CDS             complement(18161..19126)
                     /locus_tag="VFR20_06555"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU0230213.1"
                     /translation="MKPPLRIYRTLPRFDEDRPSAVTIGNFDGVHRGHQAILARVREE
                     SAARGLIPTVMTFEPHPRAFFARRGQRPELIPTQISSLRDKVWALARHGIRQIVIVKF
                     DQALADLAASDFIENFLVRGLDARWLLVGSDFRYGHKRSGDIALLRSAGSHAGFDVET
                     IADIADGDGHRISSSEVRTALAVGDLERAGSLLGHNYGISGHVVHGMKLGRTLGFPTL
                     NIHVPPQCAARSGVYVVRAHGLAERPLNGVASLGVRPTVSHAGHVLLEVHLLGSALDA
                     YGKLTRIEFLKYLRDEEKFPDLPTMIAAIGNDARCARDYFALHGL"
     gene            19173..19868
                     /gene="purN"
                     /locus_tag="VFR20_06560"
     CDS             19173..19868
                     /gene="purN"
                     /locus_tag="VFR20_06560"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU0230214.1"
                     /translation="MNASSPPSCRLVVLISGRGSNMRTIVETVRDRGLPARVGAVISN
                     QSDAEGLEWARAQGLETCVVPHRDYASREDFDHALAQAIDRYDPHFVLLAGFMRVLTP
                     GFVARYHGRLLNIHPSLLPAFPGLRTHQQALDNGVQWHGCTVHFVTPVLDLGPIVAQG
                     AVPVLDGDTADRLADRVLTAEHQMYAQVVEWLAEGRVSLDEHSRVRVRGVSTRSFVYL
                     SDSVRVVGAGEQS"
     gene            19865..21346
                     /locus_tag="VFR20_06565"
     CDS             19865..21346
                     /locus_tag="VFR20_06565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEU0230215.1"
                     /translation="MSQGRSSGKGSSGARRDRPSGTAPVRNRSSESPAPRGRSSDASA
                     PRGRPSAAGTPGDGRARRASAGPRAVENRPSTAAIRIGQVQDALGEVLQWSLPADAAL
                     SRWLRAHPKLGARDRAEVADSVFEALRHLRRYRQFAESGSGPASRRLAILALSSVLSG
                     PALREALSEPEKGWLDHVEGIDPAGLPRAVRASLPDWLDARLAELPDADSLITALNQT
                     APLDVRANPFKIERDALLDSLRGGAGLRFDPRPTPYSPWGIRLQGRPPVNRWPMFERG
                     ELEVQDEGSQILVALVAPRRGDMVIDYCAGAGGKTLLLGALMRSTGRLYAFDVSAVRL
                     ARAKPRFARSGLSNIVPVVIAPENDQRVKRLRGKAQRVLVDAPCSGLGTLRRNPDLKW
                     RQHPDSLTQLLATQASILSQASRCVAPGGRLVYGTCSVLPEENERQVERFLAENQDFT
                     LLDAGPVLADRCKTLTLSGPYLSLRPDHHGTDGFFGAVLERAG"
     gene            21593..22810
                     /locus_tag="VFR20_06570"
     CDS             21593..22810
                     /locus_tag="VFR20_06570"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEU0230216.1"
                     /translation="MDQILNFLSGGVLQWSWWQVLLFTLVVTHITIVAVTIFLHRSQA
                     HRGLDLHPAVMHFFRFWLWMTTGMVTKEWVAIHRKHHAKCEREGDPHSPMVYGIGKVF
                     FRGAELYRDEALNAETLKKFGHGTPDDWLERKLYARHSVLGVSIMMVIDLALFGVLGL
                     TVWAVQMAWIPFWAAGVVNGLGHFWGYRNYACPDASTNVSPWGIIIGGEELHNNHHAF
                     GTSAKFSTKWYEFDIGWGYIRILSFLRLATVKKVAPKLRLEPASKLAPVSLSTLQGVI
                     THRYEILARYTDMVKRSVSDELARLKPAARQEQGGQYSKSLPRRVKEWLSHGDEALEP
                     EQRAELDTVLARNDSLSTLVQMRRELVRLWESSSASSEQLLSDLQAWCQRAQQSGIEG
                     LEQFALRLRRYAA"
     gene            22807..>24371
                     /locus_tag="VFR20_06575"
     CDS             22807..>24371
                     /locus_tag="VFR20_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU0230217.1"
                     /translation="MIDQLNPQQQSAVTLPPQHALVLAGAGSGKTRVLTTRMAWLIQT
                     GQASPFSLLAVTFTNKSAREMLTRLSALLPINTRGMWVGTFHGLCNRLLRAHHRDAGL
                     PQTFQILDTGDQLSLVKRLLKGAGIDDEKFPARDVQRFINSAKERGERPDAVEAWDAH
                     QRQLVEIYRLYQAQCEREGVVDFAELLLRAFELLQHNGPVREHYQRRFRHILVDEFQD
                     TNTLQYRWLKLLAGGGASIFAVGDDDQSIYAFRGANVGNMADFQREYARETVIRLEQN
                     YRSYGHILDSANALIAHNSARLGKNLWTEQGEGEPVRVSEQPSDLLEAQWVVDEIKAL
                     VDEGSARREIAILYRSNAQSRVLEHALFSARIPYKVYGGLRFFERQEVKHALAYLRLL
                     ENPHDDTSWLRVVNFPARGIGARTLEQLSDAARANQCSLFRAVPHLSGKGGGNLAKFA
                     ELIAQMAHEAQVLSLPELVDHVLHHSGLLAHYQGERDGTDRVENLQELVNAAQVFVFD
                     ENFDGLPAGRVVDA"
BASE COUNT         4228 a         7482 c         7935 g         4726 t
ORIGIN      
        1 ctcgaatcag gcagccgtaa tgttgagagc ttgcttccct ttggggcctt gggcgatttc
       61 gaacgcgacc ttctggcctt ccttgagcgt cttgaaaccg ttcatttgta tggatgaaaa
      121 atgggcgaaa agatcttcgc cgccgttgtc tggggtgatg aagccaaagc ctttagcatc
      181 attgaaccat ttgacggttc ccgtcagctt ttgggtatcc ccggagggtg ctgttgaatc
      241 tgacatgcgg actgcctctc gactatagtg ttacgttaaa acccgggctg tcggccggac
      301 accagcggca cgggattctg gaaatgtcaa ctgatacatc tcagaatagc gtgatgatga
      361 gtactttacc ccgccacagt caactatgga aagcacttat attgatgctg tttcatgttt
      421 tttttctatg atttgacgtc atggccaccc aaaccgaaag acaacgcgaa gtcgtggtcg
      481 aaagacagga agaacgtctg gcaccgccac ccatgtatca ggtcgtgctg ctcaacgatg
      541 attacacgcc gatggaattc gtggtggacg tcctgcggcg gatattcggc aagagcgaag
      601 acgaggcctc gcgcatcatg ctcaaggttc accatgaggg tcgcggagtg tgcggggtgt
      661 ttccccgcga ggtggccgaa acccgggtcg agatggtccg aagatccgcc caggagcgcc
      721 agcatccgtt acagtgcata atggaaccgg cgccggccga ttgaacatgg ttgtcgtgac
      781 gtcatgtttt tgaggctatc cggtcacact gtccacggaa tcgccgctac gcaccgacga
      841 tttagtcata gaataggatg tatcgattcg tcgatatgtt tgtccgcttc gtatatggag
      901 gaagcgtgat ttccgaagag cttgaagtca gcttgcacat ggccttcgtc gaggcccgca
      961 cagccagaca cgaattcatc actgtggaac atctgctgct gtcgttgctt gacaacgtcg
     1021 cggccgttga ggttctgcgc gcgtgttcgg ccaacatcga ggcgctgcgc cgtgatctgc
     1081 gcaagttcat caacgagaac acgcccgtct ttcctggcga gggcgaggtc gacacccagc
     1141 ccacgctggg gttccaacgc gtcattcagc gcgccatcat gcatgtctcg tcgggcaatt
     1201 ccaccaagaa aagcgtcacc ggcgccaatg ttctagttgc catgtacgga gaaaaggatt
     1261 cccatgcggt gtactacctg ctgcagcagg gtgtcactcg cctcgatgtg gtcaacttcc
     1321 tgtcgcatgg catcaccaaa gccagcgacg attccagccc cgaaccggtc aagcaaccgg
     1381 gggcggaaac cgagcacaag cccgagcagc ataaatcgcc gcttgagctt tatgcgctcg
     1441 acctcaattt cgaatcccgt gaagggcgca tcgacccgct cattggtcgc gaggccgagg
     1501 tcgaacgcgt catccaggtg ctgtgccggc ggcgcaagaa caatccgctg ctcgtgggcg
     1561 aggccggcgt gggcaagacc gccatcgccg agggcttggc gtggcgcatc gtcaagggcg
     1621 acgtccccga cattcttgcc gacgcgcaag tctacgcgct cgacatgggg gctctgcttg
     1681 cgggcactaa gtaccggggc gacttcgaac agcgtttgaa atccgtgctg cgttcgctca
     1741 aggacaacgc caacgccata ctgttcatag acgaaatcca caccctcatt ggcgccggct
     1801 cggcatcggg cggcacgctc gacgcctcga acctgttgaa gcccgcgctg tcttccgggc
     1861 agctcaagtg cctgggcgcc accacctaca acgaataccg cggcatcttc gagaaagacc
     1921 acgccctgtc ccggcggttc cagaagatag acgtggtcga acccacggtc gagcaaacca
     1981 tacaaatcct gcgcgggctc aagcgctact tcgaggcaca ccatagcgtg cgctattcgg
     2041 cggcggcact taccgcagcg gccgaactgt cggctcgaca catcaacgat cgctacctgc
     2101 ccgacaaggc catcgacgtc atcgacgagg ccggtgcggc gcaacgcctg ttgccgcgct
     2161 cccggcagaa gaaactcatt ggcaaggccg aggtcgaagc cactgtggcc aagatcgcgc
     2221 gcattccgcc ccagagcgtt tccaacgatg accgcagcaa gctggccacc ctcgaacgcg
     2281 acctcaagac tgtcgttttc ggccaagatg cggccatcga ggcgctggcc ggggccatca
     2341 agatggcgcg ctccggcctt gggcgccccg acaaacccat cggtgcgttt ctgttctccg
     2401 ggcccacggg cgtgggcaag acggaggtcg cgcgccaact ggcgttcacc atgggcatcg
     2461 agctattgcg cttcgacatg tccgaataca tggagcgcca cgccgtgtcg cgcctcatcg
     2521 gcgcgccgcc gggttacgtt ggtttcgatc agggcgggtt gctcaccgag gccatcagca
     2581 agcagcccca ttgcgtcttg ctgctcgacg aaatcgagaa agcgcatccc gatgtctaca
     2641 acatcctgct gcaggtcatg gaccacggca ccttgaccga caacaacggc cgcaaggccg
     2701 atttccgcaa tgtcatcatc gtcatgacaa ccaatgcggg cgccgaggcg ctcaatcgaa
     2761 acaccatcgg tttcgcgaca cccgaaacgg cgggggacga actgaccgac atcaagcgcc
     2821 tgttcacgcc ggaattccgg aatcggctcg acgccatcat cagcttcaag ccgttgtcgc
     2881 gcgagatcat cctgcgcgtg gtcgacaagt tcctcatgca gttggaagac cagctccacg
     2941 agaaacacgt cgacgtggtc ttctcgcagg cattgcgcgg gcatctggcg aaggaaggct
     3001 tcgatccggc gatgggcgcc cggcccatgc agcggctcat ccaggacacc atccgtcggg
     3061 cattggccga cgaactgttg ttcggacgtc tggtcgatgg cgggcatgtg gaagtcgtcc
     3121 tcgacgaaca cgacaaggtg acactggttt tcctgcccac cgatggtaaa ccgaagcatc
     3181 tacctcccgt tgattcggaa gccgagcttg tccactgagg cgtgcagacc ccttgtcctg
     3241 tgcccgcatt gtgccctgct gcacgaacgg gtgcccgttc ctcccggggg aacggcacgc
     3301 tgcgttcgct gcgacagtgt tctgtatcgc ggcagcgtgt ggtcagtctc gcagtggctc
     3361 gctctggcgt gcaccgccct gatcgttttc gcgatcgcga actattttcc cattgtcacc
     3421 ttgcggctca agtggctgat tgccaacgcg tcgctgccgc aggcgttgat gctcacatgg
     3481 gaacagggtc gtcccgtggt cgccgtgatg accgggctgt ttggtttcct tgtcccgctg
     3541 acccagttgc tgtttctggt gtgggcgctc atgtgcgtgg cctcgggaaa cctgccgcgc
     3601 gatttcgcca tgggcatgcg ggtgttgcgc gttctgggca cctggagcat ggttcccgtc
     3661 ctgatgctgg gcatcctggt ggccatcgtc aagttggccg ggttggctgt gctgcacgtg
     3721 gcgccgggcc tctgggggtt cgtggccttg accgtgttgc tcaccctgct tgagcgtgta
     3781 tcggcggggt ggctgtggcg ccaggccgag gacgcggggc tggttccatt atccggcatc
     3841 gatgtcgacc cggcggccac cgtggcgagt tgccatgcct gtggcaatgt ccagaatctc
     3901 gcctcgccgc acgatcatca ggcttgccgt cgatgcggcg cgaaggtgag gttccggttg
     3961 ccgggcatgt cccgaacctg ggcgttggtg cttgcagcct gcatactgta tgttccggcc
     4021 aatgtcctgc cggtcatgaa gttccgcacg ccggtgggta cgaccgagca cacgattctg
     4081 ggtggtgtgg tcgaactctg gaagctggga tcctgggatc tggccatcat cgtgttcgtc
     4141 gcgagcatcg ttgtgcccat gaccaagctg ctgatcctca tcgcgctcat ggcgcgtcgc
     4201 tgctggttgg gtgacgatat acagcgtcag cgcacgcggt tgtacgaatt ggtggaattc
     4261 atcgggcagt ggtccatgct cgatgtcttt gtcgtcattc ttctgacgtc gatggcgaat
     4321 ttccccggca tttcccagat ttccgcaggc cccggagccg ccagttttgg cttggtcgtg
     4381 gtgctgacca tgctggcgtc ggccggtttc gacccgcgtc atggatggga cgcgcgtccg
     4441 tcgttgggtt cgagcgatgg gcgcaagacg gcggtgggtg gtgaacgcct ggcgggaccc
     4501 gcaacggagc atgcatgacg gaacccacac cctccgagcc agccgcatcg actgatgtcc
     4561 ccgcaaccgc agggacgcca gccgcccccc atgtcgtcgc gccgaaacgg cgcagcgtct
     4621 cctggatctg gctggtgcca ttgctggtgg cggtcgtggg tctgtcattg atcgtcaaga
     4681 actggatgga aaccggccct atcgtgacca tcagctttca gtcggccaat ggcatcgagg
     4741 tgggccagac aacggtccgt tatcgcgatg tcgtcgtcgg agtggtgaac gatatcgagg
     4801 tgtcgcccga tcgctcgagg gtgctggtca gggtgcggct caagcatgat gccgccgcgt
     4861 atatcgcccg aaaaggcaca cgcttctggg tggtgcggcc gcggctggag ctgactggtg
     4921 tgtctggact gaacaccctg ctgtctggtg cgtatatcgg tgtcgacacg tcgaggacga
     4981 aggatcacgg agtcgaaacc gccgaggcct atcgtttcat cggcctggag aaacccccag
     5041 agatcaccag tgacaagccc ggtacgcgct acaccttgac ggcccccgat ctgggctcgc
     5101 tggagttggg gtcgccggtg tactaccgcc gcatccaggt ggggagggtg atcgattacg
     5161 gtctggcccc agatggcaag ctggtcgaca tccagatttt cgtcgatgcg cccaatgaca
     5221 aatacgtcac cgccgatacc cgtttctgga acgtgagcgg gattcagttg tcggtcggtt
     5281 cggacggtgt cactgtgcag gcgggatctc tggcgtcggt catttccggc ggggtggaat
     5341 ttgcatcggc cgatgccgcc atcacgactc ccgccaaggc caacaccacc ttctcactgc
     5401 agtcaacgag gcaagcggcg atggcggacc cggatggcat cccggttccc atcaacatgg
     5461 aattccggca gtcggtgcgt gggttgagtg tgggggcgcc cgtgatgttc catggcctgg
     5521 agatcggcaa agtcacagat atcaatctcg agtacaacgt caagatccgg cggttctacg
     5581 tactggtcaa gaccgagctc tttcccatgc ggttcggcaa agtctacgaa gatctgatcg
     5641 ccgagaacga aaagagcggc aacgccggcc acaatcttct gaggccgatg atcgagcatg
     5701 gtctgcgcgc gcaattgcgc acgggcaatc tgctgaccgg acaacaatac attgcgctgg
     5761 cgttcgtccc ggatgcctcc ccggtgcatt tcgatgaaac gcagaagccc ccgctcattc
     5821 ccactgtgcc gggcagcttc gatcgtctgc aggagcaggt gagcagtctc gtgaccaagc
     5881 tcgatgcgat tccgttcgcc ggtatcagcc aggatcttcg cggagcattg aaggcattga
     5941 cccgcacgtt gaacggcctg catggcgtgg ggccgcaaac cgagaccacg ctcaaggcgg
     6001 tgcgcaagtc gctggatcag gtcagcggcc tgctcggctc ggcggcttca ggcgggaatc
     6061 tgggccagac gttgcgggag ctcgggcagg cggccaagtc attgcgggcg ctggcggatt
     6121 atctccaggc caacccgtcg gcgttgttgc gcggacggcc cgccgatgtg ttgccgggtc
     6181 attgatcctt gaggagggcg caagtcttga aatattcgta tctgatcccg accctggtgt
     6241 tgatcgccct gctgggcggg tgtgcggcgg cgccaaagat tcagtactac acgctgatcc
     6301 cacctcttgc cgacacggcc ctcgccaacg cggcatcgaa ggccaccgcg ggccggtatg
     6361 cgatcagtgt gcagcctgtc tcggtacccg accaagtcga tcggccgcaa atcgtcatca
     6421 gcggcggcga cagcgccagg gtaaccccgc tcaacgaatc cttatgggtg tcacctctcg
     6481 gcgatcaggt gcgtcgggcc ttgtcggcgg atctgtcggc gcgcctggga acgctcgaca
     6541 tcgcggccga taccgcgccg tcatcgctgc cggtatggaa gatcttcctg accgttcagc
     6601 gctttgatgc gattttcgga aggcaaacca tcctcgatgc cacctggcgt ctcgatcccg
     6661 tgcatctgcg gggcaggcga ccaaccatct gccgggccga ggtcactgtt ccagtggggg
     6721 cgggtgtgcc ggcgttggtg atggggcagc gcaaggcgtt gtggacactt tcctcgctca
     6781 ttgccgccca gatctcagga ggcccgccgg caccctcgaa ccggcctggc gtcgtgctca
     6841 agggctgcgc ctgaagcccg tgtcgagcaa ttttcttgcc gagggtgtcc gatccggtgc
     6901 tcggtttccg cgctcaagcc aggaatcccc tgaatcagtc gctgagccac atgccgctat
     6961 ggattggtgc tgtccgtcgc ccaaaaggta cagccttgca ggtatactag ggttaacctt
     7021 aattcagaaa aggttgcaac caatttagta ttttggttta ccctgagtgg ctcccgctcg
     7081 gggcaggcgc gcccgtcccg ggcgcgccgc gatcttgcac gaggcacgac aggaattgcc
     7141 atggcgacga ccacactggg tgtaaaagta gatgaggcat tgcgtgaacg gcttcggcac
     7201 gccgcgcaga aactggggtg cacaccccac tggttgcaca aacaggcctt gttcgcctat
     7261 atcgatgcca tcgaacgcgg gcaggtgccg gctgaaatca gctacctgaa tcaacaggat
     7321 gtcgcggaag aggcgggtga ggtcgcgcat caggatccgt tgccgccgtt ttacgagttc
     7381 gctcaggacg tccagcccca gtcggttttg cgtgccgcca ttaccagcgc ctatcgccgc
     7441 cccgaaaccg aatgcgtgcc gctgctggcg gaccaggcgc gcgatatccc cggccccgac
     7501 gtgcatgcgc tggccgaacg cctggtcaag acgctgcgca gcaaacgcaa gggcggcggg
     7561 gtagagggcc tgatccagga gttctcgttg tccagtcagg aaggggtcgc gctgatgtgc
     7621 ctggccgagg cgctgctgcg cattccggat cgcgccaccc gtgatgccct gatccgtgac
     7681 aagatcagcc gcggcgactg gcgtgcgcac atgggcgagt cgccttcgct gttcgtcaat
     7741 gccgccacgt ggggcctgat gctcaccggc aagctggtca gcgtcaacag cgagcagtcc
     7801 ttgtcccggg cgctgacacg tctcatcggc aagggcggcg aaccgctggt ccgcaagggc
     7861 gtgaacatcg ccatgcgcat gatgggcgag cagttcgtcg cgggacagaa catttccgcg
     7921 gcgctggcca acagccggaa gctggaggaa aaggggttcc gctactccta cgatatgctg
     7981 ggtgaggcgg ccacgaccgc cgccgacgcc gaccggtaca acacttcgta cgaacaggcc
     8041 atccacgcga tcggcaagtc ggcgcagggg cgcggtattt acgaaggccc ggggatatcc
     8101 atcaagctct cggcgctgca tccgcgttat tcacgcgctc aacgcgaccg ggtgatggcg
     8161 gagctgctgc cgcgcgtgac ggcgctcgcc cgcctggccc gcagctacga catcggcctg
     8221 aacatcgacg ccgaagaggc cgaccgcctg gagatctccc tggatctctt ggaggccctg
     8281 tgcttcgatc ccgatcttgc cggctggaac ggcatcggtt tcgtgatcca ggcataccag
     8341 aagcgcgcgc cgttcgtcat tgattatgtc atcgacctgg ccaagcgcag ccgccatcgg
     8401 ctgatggttc gcctggtcaa gggggcgtac tgggacagcg agatcaagcg ggcacaagag
     8461 ggcggcctgg aaggctatcc ggtctacacg cgcaagattt acaccgatat ctcgtatctg
     8521 gcttgtgcgc gcaagttgct ggcggtgcct gacgccgtct atcctcagtt cgcgacgcac
     8581 aacgcttata cgctggcatc catttaccat ctggcggggc agaattatta ccctgggcaa
     8641 tacgagttcc agtgcctgca cggcatgggc gaatcgcttt acgagcatgt caccgggccg
     8701 gtcgcgcaag gcaagctcaa ccgcccctgc agaatctatg cgccggtggg cacgcacgag
     8761 acgctgctgg cctacctggt gcgccgtctg ctcgagaatg gcgccaatac ttcgttcgtc
     8821 aaccagattg gtgacgacga cattcccgtc gaggcgctca tcatcgatcc ggtgcaggcc
     8881 gcgcagcgca tacagccgct gggtgccccg catgacaaga tccctctgcc cagggacctg
     8941 taccaggcca gggacggccg cagcaactcc atggggctgg atttgagcaa cgagcaacgg
     9001 ctcgggtcgc tgtcggccgc gctgttgggc agtgccacgg ccacgtggcg ggcccaaccg
     9061 ctgctgggcg gcgcccagcc cgagtgggac gactcccgcg cgcagccggt gcggaatcct
     9121 gctgatcgcc gtgatgtcgt ggggcacgtg atcgaggcgt ccgacgccga agtccgccag
     9181 gcgttgcagg aggcggcgca tgcggcgccc atctggcagt ccaccccggt cgaggaacgc
     9241 gcgcagtgcc tggaacgggc ggcgcagatg ctcgaggacg acatgcagcc gttgttgggg
     9301 ctgatcgttc gcgaggccgg gaagtcgctg cccaacgcca tttccgaagt ccgcgaggcc
     9361 accgatttcc tgcgttatta cgccggccag atccggagcg cattctccgg cgacacgcat
     9421 cgcccgctgg ggcctgtcgt gtgcatcagc ccatggaact tcccgctcgc catcttcacc
     9481 gggcaggtgt ccgccgcgct cgccgcgggc aatcccgtgc tggccaaacc ggcggagcag
     9541 acctgcctga tcgccgccgc ggcggtggcc atcctgcgca aggcgggggt tccatcgggc
     9601 gctgtgcaac tgttgccggg tctgggcgag accgtgggtg cgagcctggt ggccagcccc
     9661 gtcgtgcgtg gtgtgatgtt cacgggctcg accgaggtgg cgcagatcat ttcccgcacg
     9721 ttggcgcaac gacttgacga cgccgggcat cccattcccc tgatcgccga gaccggtggc
     9781 cagaatgcca tggtcgtcga ttcatcggcg ctggccgaac aggtcgtgtt cgacgtgctg
     9841 acttccgcct tcgattccgc cggacagcgc tgctcggcgc tgcgtgtgct ctgcgtgcaa
     9901 gaggattgcg ccgatcacgt gttgggcatg ctgcgcggcg cgatgcagga attgcgtgtc
     9961 ggcaatcctg acatgctctc caccgatgtc gggccggtca tcgacatgca ggcgcaggct
    10021 gtcatagaaa agcacatcgc cgccatgcgt gacgcgggcc gacgcgtgga tcaactcggt
    10081 ctggattcca tgtgccagca tggctccttc gtggcgccca ccatcgtcga actcgatgat
    10141 atatcggcct tgaagcgcga agtgttcggg ccggtgctgc atgtgatccg ctacaagcgg
    10201 gacgggctgg acgcgctgat cgacgcgatc aacgccactg gctatggcct gacgttcgga
    10261 gtgcacaccc gcctggacga aaccgtggcc cgtgtgacga gccgcattca tgcgggcaat
    10321 gtctacgtca atcgcaatat cgtgggcgcc gtggtgggtg tccagccgtt tggcggcgaa
    10381 ggcctctcgg gcaccggacc caaggcgggc ggccccttgt atctgttgcg gctgttgtcc
    10441 cgccgtccgg cggggctacc cgactggtgg caagagccgg atgccggcgg tcgggaactc
    10501 gagttgcccg gccccaccgg ggaaaccaac acctatcatc tcaagccgcg cggcacggtg
    10561 ctgggggtgg cggcatcgcg ggaaggcgcc gtggcccaat atcaggcttg tgtcaccact
    10621 ggcaacgcgc tggcgctggc cgagacggat gcggcgcggg cattcctggc cgattgcacg
    10681 ccggagcaaa agaaacgggt gctcttggtg ccccctggcg acatcggcca ggcggattac
    10741 caggcggtgc tgttcgaagg ggacggcgac gccttgcgcg aactcaatca ggtggtcgcc
    10801 gcgcgctccg ggcccattgt ctcggtgcag gggctgagca cggacgaact ggcggccggg
    10861 catcagtact cgctggaacg gctgttgcgc gaggtgtcgg tcagcgtgaa cacggcggcg
    10921 gctggcggca acgccagcct gatgatggtc ggctgatccc tgggccgctg tccggggctc
    10981 gccgcatgtt ttcagtgcgg tgggcctttt cgttccatgc gagggggagg gggcggtgtc
    11041 tccgctgggt gctacgcctt gcctgtgctg ccgaatccgc ccgcgccgcg ctcggtttca
    11101 atggtgaacg cgtccacgac gtgcaggtcc acctgaacca ccggctggaa catcaactgg
    11161 gcgatcctgt cgccgggttc cacgcgatag ggcgtgtcgc tgtcgttcgc gaggatgacg
    11221 gcgatctcgc catggtagtc cgagtctatg acgccgatgc cctgggcgac acgcacgccg
    11281 tgcttgagcg acaggcccga gcgggacgcg acgatcgcca ccagccccgg gtccatcatg
    11341 ttgagggcga ttcccgttgg caccagggtg cggccaccgg gcgcgaccgt caccgcttcg
    11401 tccaggcagg cgcgcaaatc catggccgcc gacccgtctg tggcatagcc gggcgccgga
    11461 atgcgggtgc ccagcaaggg gttcacgatc cgggtttcga tgcgtctgaa catgggaatc
    11521 ctttctgaaa tgattgtctg gcgctctgta tagcacaacc cgccggcttt ccgcacacgc
    11581 cgtgcggggc tggccgttgt gatatagtcc gatcgcgggt tcgctccctg tcatgtcgga
    11641 gcggttcgcg cgttctcagg gcggggtgtg attccccacc ggcggtaatg gctgcaatgg
    11701 cctagcccgc gagcgcctga aggtgattcc ctccctattc ccgggtaagg gttcgccggc
    11761 agggtcagca gacccggtgt gattccgggg ccgacggtga tagtccggat gaagagagag
    11821 cgggatgccg ccggccacgc cggcgaatgg ggtgtttgcc attggcctgg tcaatgggtg
    11881 cgggcatcac cttttccctg tctgtccaag ccctgttttt atggttctga aaacaggaga
    11941 attcagtatg tccaatcctc gatccggctg ctcaggcgct gtcgcgtcgg cccggtccat
    12001 tgcgttcgtg caggcgggct ggcacagcga tatcatcgcg caggcgcgcc tgtcttttct
    12061 cgacgagatc acccgttcag gcttcgaccc gcgggacatc catgtcatcg atgtgccggg
    12121 tgccttcgaa atcccgctgc acgcccgtct gctggcggaa accggacgct attcggcgat
    12181 cgtcgccgcg ggcctggtgg ccgatggtgg catctatcgg cacgagttcg tcgcgcaggc
    12241 cgtcgtcagc gggctgatgc aggttcaact cgagacccgc gtgccagtcc tgtcctgcgt
    12301 gctgacgccg caggaacggt tcgaaggtga cgatctgcat cgtttctttt tcgaacattt
    12361 ccggatcaag ggtgtggaag cggcgcgagc ctgcgcggcg actctttctt ccctggaaac
    12421 gcttcaattg atcggagggt gagagtatgt tcacgggaat cgtccaaggg atggccacca
    12481 tcgacagcat tgacgaccgg gagggcctgc gcactttcag cctgatgttt cctcccgggt
    12541 tctgcgacgg gctggcggtg ggggccagtg tgtctgtgga tggggtctgc ctgacggtga
    12601 ctcacctgct gtcggccaat gcggccagct tcgacgtgat gctcaaaagc ctggacctga
    12661 ccacgctggg cggctatgtg cgtggcgggc gggtcaacgt cgagcgcgct gcgcgtgacg
    12721 gcgccgagat aggcgggcat ccgctttcgg gccacgtcga tttccacgcg cccctggtgg
    12781 cggtgcggca gatggataac aaccgtgtcc tgcgcatcgg cattccccct ggcttccgca
    12841 agtacgtctt cgccaaaggc tatattgcgg tcaatggcgc cagcctgacc gtggccgacg
    12901 tcaatcgcga cgaaggctgg ttcgaggtct ggctcattcc cgaaacgcgt cgtgcgaccg
    12961 tgtttgaaca gaagcagacg ggtgatgcgc tgaacgtcga aatcgatcgc ggcacacagg
    13021 tgatagtcga cacggtgcgc gagacggtgc tggaatgcct agggggcctg cagcccgttc
    13081 tggaagccgc gctgagggac aaggggctgg agatcgagga tctgctgcgt ttgccaccca
    13141 gggcctgaat cggtgcgcgg ggggcgcccc cgcgatgatc cagcgctgtg cccgggtcaa
    13201 tgctcgatat gatcgggcga agaggtttca ggcagttccg gtgtgacctc cggattgagc
    13261 gcggcacgcg cgccgagcag caggatcacg ccagccacca gcatgatgac ggccggcgcc
    13321 cagtcgtccc aggcgccgcc ctcgttgaac aggcgccagc cgttcagcag cgcgtcgacg
    13381 atcagcacca cgctggccac cgccagcacc agcgcgatca gtcggcgcgg catggaaacc
    13441 gcgcgtacct gacccgcgtt gtcacgacgg gcagaggagg ccgggttgcc aagaggatgt
    13501 cgtatcacgc caggatccgt ccgtttgtca tcgtgggttg ttgggcaggc gcgcggcgat
    13561 ggcacgcatc agttgccggg cggcctcggg tttgggcagg cgtggcagcg gatgtgcgcc
    13621 ggtgtcgtcg aacaacacca gttgcgtgtc gtccgccccc atggattgct gagccaggtt
    13681 gcccaccatc agcgccacgc ccttgcgtgt gcgcttggcc tgcgcgtagg cctcgagatt
    13741 ttctgtttcg gcggcgaacc cgacacacca cggtccgttg ggccgcgcgg cgacgtccgc
    13801 cagtatatcg ggattggggg cgaactccag atcgaacggc gccgcctggc cgcgtttctt
    13861 gattttttgc gtactggggt gaacgacatg ccagtcggcc accgcggcga cggcaatgaa
    13921 aatatccgca tcagcgaccg cttccatgac ggccgcatgc atctggcgtg cgctgtcgac
    13981 gtcgatgcgt gtgacaccat agggggcttg aagtgccgtg ggacccgaca ccagcacgac
    14041 atccgctccc gcttgccgcg cggcgcgcgc cagggcgtag ccggtcttgc cggatgaacg
    14101 gttggtgatg acgcgcaccg gatccacggt ttcggatgtg gggccggcgg tgatgacgat
    14161 gcggcggccc cgcagatctt tcggttggaa gctgccgatc agttcgtcga gcagttcaag
    14221 aggttcgagc atgcggccat cgcccacttc gccgcatgcc tggctgccgc ttccgggccc
    14281 cagaatgacc acaccgtctt cggccagttg ccggatattg cgctgggtgg gcgggttgag
    14341 ccacatttcg cgattcatgg ccggcgccaa caacagtggg caggccccgc gggcggcgca
    14401 cagggtagac agcaagtcat tggccagccc gttggccagc cgggcgatga aatcggtgct
    14461 ggccggcgcg accaggatgg catcggcccc gcgcgtgaga tgtatgtgcg ccatgccgtc
    14521 ggacggaccg gcatcgaagg tgtccacgta caccggacgc ccggaaagcg cttgcatggt
    14581 ggttgtcgtg atgaagcgcg tggcagcctc ggtcatgacc acgtcgaccg tgccgccctg
    14641 gtcgagcgcg cgccgcagga actccgccgt cttgtagcag gcgatcccgc cggttaggcc
    14701 cagtacgatt cgtttgtcgg caagttcccg catatgctgg tccggttttt caagagatgg
    14761 gcaggccggc gtgttttgtc tggttgattg aatgtacaag ccagccagac cggatgctaa
    14821 cccgccttgt ttcgatcacg ccgcatgcgc aggagctcgt cgaggatgag cagggcggcg
    14881 ccacaggtga tggccacgtc agcgacattg aacgcgggga agtaccagtc gcgccagtag
    14941 aacaggaaga aatcgatgac gtgcccgtgc tgcaggcgat ctatgacatt gcccacggcc
    15001 cccccaagta tggccgtgat cgatgcgcag aacaaggtct ggccaggatt cttgcgcagc
    15061 agatggatga tgagtcccgc ggcggccagc gcaatggcgg tgaacagcca gcgctgccac
    15121 ccgccatccg tcgcaaggaa actgaatgcg gcaccggggt tgtagagcag ggtgatgtcg
    15181 aacaccggca gcacatgcag gcgttcaccg tactgcaaat gcgagttgac atagagcttg
    15241 ctgacctgat ccaggacgat gatggccagc gcgccggcca gccagaccca gagcttcgcc
    15301 cgatggcgcg cgccgacgcc gcgtagtgtt gaatcaggca tgttgacgcg cttcgccagt
    15361 gccgaacagg ttgtcagtgc atcgtccgca gagatccggg tgggccgcgt ccgtgccgac
    15421 atcggcgcgg tgatgccagc aacggccgca cttcggttgt gtcgtggcgg cgacgtcgat
    15481 ggtgatcggc cccgacgcct cgtgcagcga ggcgcgcgag acgatcaaca cgaaacgcag
    15541 gtcgtcgccc aggctgtcga gcagcttgtg gtcatcgccg tcggcgtgga tgtcgacttc
    15601 ggcgtccaga gacgacccga tggcgccggt gccgcgaact tcctcgatct tgcgcatggc
    15661 gtcggctcga atggcgcgca gccgcgtcca tttgtcggtc agcagccccg catcgggttg
    15721 tgccggaatg gcgtggaaca gctctgtgaa gatggtttgt gtttcctggc tcgcatgcat
    15781 gtcgctccat gcctcttcgg cggtgaacga cagaatgggc gccatcagtt tgagcagcgc
    15841 gtgcgtgaca tggaacagcg cggtctgcgc ggaacggcgc gcgacgctgt cggcgcccgt
    15901 ggtgtagagc cgatccttca gaatgtccag atagaacgcg cccaggtctt cggagcagaa
    15961 aatctgcagt cgcgaaaccg ccaggtggaa ctcgtagcgg ttgtaggagg ccaacacctc
    16021 ggactgcatt tgcgccgtca aggccagcgc atagcggtcg atctcgaaca ttttttccaa
    16081 cggcagggcg ttggcgcggg cgtcgaagtc cagcagattg cccagtagga acttcagcgt
    16141 gttgcggatg cggcggtagc tctcgaccac gcgcttgagg atctcgtcgg aaatcgtcag
    16201 ttcgccggaa tagtcggtgg acgccgtcca gagccgcagg atttccgccc ccagggtgtc
    16261 ggacactttc tgcggcgcaa cgacgttgcc gacggatttg ctcattttcc ggcccttgcc
    16321 gtcgaccacg aagccgtgcg tgagcaacgc cttgtagggg gggcggccgt acagcatgca
    16381 gcccgtgagc agcgacgaat ggaaccagcc gcggtgctga tccgagcctt ccaggtagag
    16441 atcggccggc caattgagtg agtccgcgtg cgaacccttg agctgatggt cgcggccgcc
    16501 cagcacggtg gcgtgggtgc tgccggagtc gaaccagaca tcgagtgtgt cgcggttctt
    16561 ttcgtattgg tcggcttcgt cgcccagcaa ctcacgcggg tccagcgttt gccaagcctc
    16621 gatgccctgt ttttcgacgc ggcgggccac ttcttcaaga agctcgacgg tgcgcgggtg
    16681 cagcgcaccg gtttccctgt gcacgaagaa tgccatgggc acgccccatt ggcgttggcg
    16741 cgagagcgtc cagtcagggc ggttggcgat catggcgtac aggcgcgcgc ggccccacga
    16801 cgggaagaat tcggtctgct ctatgccttg cagcgcggtt tcacgcagcg atgccccgga
    16861 tttgggttgc aggtccagac ccgcgaacca ttggctggtc gcccggtaga tgatgggcgt
    16921 cttgtggcgc cagcaatgca tgtagctgtg cttgtgctgc tcgaccttga gcagcgcgcc
    16981 gctgttttgc agcgcctcca cgattttcgg attggctttc cagatcatca agccgccgaa
    17041 caacggcaat gatgggttgt aatggccatc gcccatcacc gggttgacga tctggtcgtc
    17101 ttccagacca tgtgccttgc acgaaatgaa gtcttcaacc ccgtaggcgg gcgatgagtg
    17161 gacgatgccg gtgccggcat cgagcgtgac gtagtcaccc aggtagaccg gtgacaggcg
    17221 ccggtagccg gggtcgaggt cgtgcagtgg gtgccggaat tccaggttgg ccagcgccgc
    17281 gcctttgacg gtggcgacga tgcgcccttc gagttcccag gcttgcaggc aactttcgac
    17341 gagatcggtg gcgagcagca gcatgctacc cgtggcacgg ggctgggcca ggcgcaccag
    17401 agaataatcg atgtcggcgc cgatgttcag ggcctggttg gccggcagag tccagggtgt
    17461 ggtcgtccag atcacggcgg cgccatcgtc gatctgcggc agcccgaagg catgggccag
    17521 tttttccttg tcgtggaacc gcagcgccac gtagaccgcc ggatcggtgc gatcggcgta
    17581 ttcgacctcg gcctcggcca gcgccgatcc gcagtcgaag caccagttca cgggcttgag
    17641 gccgcggtac acataccctt tctccatgat gcgggccaac acacggagct cgtcggcttc
    17701 gttgctgtga ttcatggtga gataggggtg gtcccagtcg cccagcacac ccagccgctt
    17761 gaagtcttgc cgctggcggt cgatctgttc gagcgcatac gcacgggccc gggactgcac
    17821 ttcttccacc ggcaggttct tgccgtattt cttttcgatc tggatttcga tgggcatgcc
    17881 gtggcagtcc cagcctggca cgtagggggc atcgtagccg gccatcgtgc ggctcttgac
    17941 gatgatgtcc ttcagaatct tgttgacggc gtggccgatg tggatgtcgc cgttggcata
    18001 gggcgggcca tcatgcagca cgaatttcgg ccgccccttg ctggcggcgc ggatggacgc
    18061 gtacaccttg ttttcttccc actgcgtgat ccagcccggc tcgcgcttgg cgaggttgcc
    18121 gcgcatgggg aacggggatt cgggcaggtt cagggtgtct ctatagtcca tggagtgcga
    18181 aatagtcgcg agcgcagcgc gcatcgttgc cgatggctgc gatcattgtg ggaagatcgg
    18241 gaaatttttc ttcgtcccga aggtacttga gaaattcaat acgcgtgagt ttaccgtatg
    18301 cgtcgagcgc gctgccgagc agatggactt ccaacagcac atggccggcg tggctgacag
    18361 tgggtcgcac ccccaggctg gcgactccgt tcagtgggcg ttcggccagg ccgtgggccc
    18421 ggacgacgta cactcccgag cgggccgcgc actgtggcgg cacgtggatg ttcaacgtgg
    18481 gaaatcccag tgtgcgcccg agtttcatgc catgcaccac gtggccgctg atgccgtagt
    18541 tgtggcccag cagggatccc gcccgctcca gatcgcccac cgccagcgcg gtgcgcacct
    18601 cggagctgga gatgcggtgc ccatcgccgt cggcgatgtc ggcgatggtt tccacatcga
    18661 aacccgcgtg gcttccggcg ctgcgcagca aggcgatgtc gccgctgcgc ttgtggccat
    18721 agcggaaatc ggatcccacc agcagccagc gcgcatcgag gccgcgcacc agaaagtttt
    18781 caatgaaatc agaggcggcc aggtccgcca aggcctggtc gaacttcacg atgacgatct
    18841 ggcggatgcc atgccgcgcc agcgcccaga ccttgtcgcg caggctgctg atctgggtcg
    18901 ggatcagttc agggcgttga ccacgccgcg cgaagaaggc gcgcggatga ggctcgaacg
    18961 tcatgacggt ggggatcagg ccgcgagccg ccgactcttc gcgtacgcgg gcaagaatcg
    19021 cctggtgccc cctgtgcaca ccgtcgaaat tgccgatggt cacggcgctg ggccgatctt
    19081 cgtcgaagcg cggcagggtg cggtagattc gtaagggagg tttcacgtcc gagagtttac
    19141 aattttggat ggcccagccc tggaattata ttttgaacgc ttcctctccc ccctcttgcc
    19201 gactggttgt cctgatttcc gggcgcggct ccaacatgcg gaccatcgtc gaaaccgtgc
    19261 gcgatcgcgg cctgccagcg cgggttggcg cggtgatttc caaccaatcg gatgccgagg
    19321 gcctggaatg ggcgcgtgcg caggggctgg agacatgcgt cgtcccgcat cgcgactatg
    19381 cctcgcgcga agacttcgat catgcgctgg cgcaggcgat cgaccgatac gacccgcatt
    19441 tcgtgctgtt ggcggggttc atgcgcgtgc tgacgccggg cttcgtggcc cggtaccacg
    19501 gccggctact caacattcat ccttctttgc tgcccgcgtt tccggggctg cgcacacacc
    19561 agcaggcgct cgataatggt gtgcaatggc atggctgcac cgtgcatttc gtcactccgg
    19621 tgctcgatct ggggcccatc gtggcacagg gcgccgtgcc ggtgctggat ggcgacacgg
    19681 ccgataggct ggccgatcgc gtgctgacgg ccgagcatca aatgtatgcg caggtcgtgg
    19741 aatggctggc cgaggggcgg gtgtcgctgg acgagcattc gcgtgtgcgg gtgcgagggg
    19801 tctccacacg ctcctttgtt tatctgagtg acagtgtgcg ggttgtcggt gccggagagc
    19861 aatcatgagt caaggtcggt catcaggcaa gggttccagc ggcgccaggc gcgatcgacc
    19921 gtcgggtacg gccccggtgc ggaaccgatc gtcggaatcg cccgctcccc gcggccgctc
    19981 atcagacgca tccgcgccgc gaggccggcc gtcggcagcc ggcacgccgg gtgatggcag
    20041 ggcgcgtcgc gcatcggcgg gtccccgggc tgtcgaaaac cggccctcca ccgcggccat
    20101 ccgcatcggc caggtgcagg acgccctggg cgaggtgttg caatggtcgc tgcctgccga
    20161 cgccgcgctg tcgcgctggt tgcgcgccca tcccaagctg ggcgcgcgcg atcgcgccga
    20221 agtcgccgat tccgtcttcg aggcactgcg gcatttgcgc cggtaccgcc agttcgccga
    20281 gagcggcagc ggtcccgcga gccgccgact ggccatcctg gccctgtcct ctgtgttgag
    20341 cggccccgcc ctgcgcgagg ccttgtccga accagaaaag gggtggctgg atcatgttga
    20401 agggatcgat cccgccggtc tgccgcgcgc ggttcgcgct tcgctgcccg actggctcga
    20461 tgcccgcttg gccgagttgc ctgatgcgga ctccctgatc acagctctga accagaccgc
    20521 gcccctggat gtgcgcgcca atccgttcaa gatcgagcgc gacgcgttgc tcgacagcct
    20581 gcgcggcggt gccggcctgc gtttcgaccc gcgccccacg ccgtattcgc cgtggggtat
    20641 tcgtctgcag ggtcgcccgc ctgtgaatcg ctggcccatg ttcgagcggg gcgagctcga
    20701 ggtccaggac gaaggcagtc agatcctggt ggcgctggtg gcgccccggc gcggcgacat
    20761 ggtcatcgat tattgtgccg gcgcgggcgg gaagaccttg ctgctggggg cgctcatgcg
    20821 ctccacgggg cgtttgtacg cgtttgacgt gtccgccgtg cgcctggcgc gcgccaagcc
    20881 gcgtttcgcg cgcagcgggc tgtccaatat cgttccagtg gtcatcgcgc ccgaaaacga
    20941 tcagcgcgtc aagcgtctgc gcggcaaggc gcaacgtgtg ctggtggacg cgccttgcag
    21001 cggcctgggc acgctgcggc gcaaccccga cctcaaatgg cgccagcacc ctgattcgct
    21061 gacgcagttg cttgccaccc aggccagcat cctgagtcag gcatcccgct gcgtggcccc
    21121 cggggggcgg ctggtctatg gcacatgcag cgtcctgcct gaggaaaatg aacggcaggt
    21181 cgagcgattc ctggcggaaa atcaggactt tacgctcctt gacgcggggc cggtcctggc
    21241 tgatcgctgt aaaaccctca ctctgtccgg cccttacctg tcgctgcggc ccgatcacca
    21301 cggcacggac gggttcttcg gcgctgtgtt ggaaagagcc ggctaatgcc gtccttgggg
    21361 ggaatggtgc ccgggggcta cccattcgcc ctgggatcgg ttattatgtt gcaatgcagc
    21421 aaaaatgggg cggcccgaca cggcgaacgc ttgtgaaatc caggaaaacc ctttcaggaa
    21481 acgaggttat gccctgatgt ggcgcaaaag ccaatttgcg ttttttctgt caaacagctg
    21541 acaagtgcgc taaactttgt tgtatcttct gacgaggtct gacaagactg gtatggatca
    21601 aattctgaac tttctttccg gcggtgtgtt gcagtggtcg tggtggcagg ttcttctgtt
    21661 caccctggtc gtcacgcaca tcaccattgt cgcggtcacc atcttcctgc accgcagcca
    21721 ggcgcaccgc gggctggatc tccatccagc cgtcatgcat ttcttccggt tctggttgtg
    21781 gatgaccacc ggcatggtca ccaaggaatg ggtggccatt catcgcaagc atcatgccaa
    21841 gtgcgaacgc gagggtgacc cgcactcgcc catggtctac ggcatcggca aggtgttctt
    21901 tcgcggcgcc gaactctacc gtgacgaagc cctcaacgcc gaaaccctga agaaattcgg
    21961 tcacggcacg cccgacgact ggctcgagcg caaactctac gcgcgccaca gcgttctggg
    22021 cgtgtccatc atgatggtca tcgacctcgc gttgttcggc gttctcgggc tgaccgtctg
    22081 ggccgtgcaa atggcctgga ttcctttctg ggccgctggc gtggtcaacg ggttggggca
    22141 tttctggggc tatcgcaatt acgcctgccc cgatgccagc accaatgtgt cgccctgggg
    22201 catcatcatc ggcggcgaag agctgcacaa caaccaccat gccttcggca cgtcggccaa
    22261 gttctcgacc aagtggtacg aattcgacat tggctggggc tacatccgca tcctcagctt
    22321 cctgcgcctg gccaccgtca agaaggtcgc gccgaagttg cgtctggaac cggcatccaa
    22381 gctcgctccc gtcagtctca gcacgctgca aggcgtcatt acgcatcgct acgaaatcct
    22441 ggcgcgctac accgacatgg tgaaacgttc cgtcagcgac gaactcgccc gtctcaagcc
    22501 cgccgcccgc caggaacaag gtgggcaata cagcaaatcg ctgccccgcc gcgtcaagga
    22561 atggctcagc cacggtgacg aggcgctcga gcccgaacag cgcgccgaac tcgacaccgt
    22621 cctggcccgc aatgactccc tgtccacgct ggtgcaaatg cgccgcgaac tcgtgcgtct
    22681 ctgggaaagc tccagcgcca gcagcgagca gcttctcagc gatctgcaag cgtggtgcca
    22741 gcgggcccag caaagcggca ttgaaggcct ggaacagttt gccctccggt tgcgccgcta
    22801 cgcggcatga tcgaccagct caatccacaa cagcaatcgg cggtcaccct tcctccccaa
    22861 cacgcgctgg tcctggccgg cgcgggtagt ggcaagaccc gggttctgac cacgcgcatg
    22921 gcctggctga ttcaaaccgg ccaggccagc ccgttttcgc tgcttgccgt cacgttcacc
    22981 aacaagtcgg cgcgtgagat gctcacgcgc ctgagcgcgt tgctgcccat caatacccgc
    23041 ggtatgtggg tggggacgtt ccatggcctg tgcaaccgtc tgctgcgtgc gcatcatcgg
    23101 gacgcgggcc tgccccagac tttccagata ctggataccg gcgaccagtt gtccctggtg
    23161 aaacgcctgc tgaagggcgc tggtatcgat gacgagaaat tccccgccag ggatgtgcag
    23221 cgtttcatca attcggccaa ggagcggggt gagcgtcccg acgcggtcga ggcctgggat
    23281 gctcatcaaa ggcaactggt cgagatctac cggctgtatc aggcgcagtg cgaacgcgag
    23341 ggggtcgtcg atttcgccga actgctgctg cgggcattcg aactgctgca gcataacggg
    23401 ccggtgcgcg agcactacca gcgccgcttc cgccacatcc tggtggatga gttccaggat
    23461 acgaacacct tgcaatatcg ttggctcaag ctgctggcgg gtggtggcgc gtccattttc
    23521 gccgtgggcg acgatgacca gtccatctat gcgtttcgcg gggccaacgt cgggaacatg
    23581 gccgatttcc agcgcgagta cgcgcgtgag acggtcatcc gtctggagca gaattatcgc
    23641 tcatacggac acatcctcga ttcggcgaat gcgttgatcg cccataattc cgcacgcctg
    23701 ggtaagaatc tgtggaccga gcaaggcgag ggcgaacccg tgcgcgtctc cgagcagcct
    23761 tccgatctgc tcgaggcgca gtgggtggtc gacgagatca aggcgttggt cgacgaaggc
    23821 agcgcccgcc gcgagatcgc catcctgtat cgcagcaatg cacagtcccg cgtgcttgaa
    23881 cacgccttgt tctcggcccg cattccatac aaggtctatg gcggcctgcg cttcttcgag
    23941 cgccaggaag tcaagcatgc gctggcctat ctgcgcctgc tggagaatcc ccacgatgac
    24001 acgtcatggc tgcgtgtcgt caatttcccc gctcgcggca tcggcgcccg cacgctcgaa
    24061 caattgtcgg atgcggcgcg tgccaaccag tgcagcctgt tccgggcggt gcctcatctg
    24121 tcggggaagg gcggtggcaa tctggcgaaa ttcgccgagc tcatcgcgca gatggcgcac
    24181 gaggcgcagg tgctgtccct gccggaattg gtcgatcacg tcctgcatca cagcggcttg
    24241 ctggcgcatt accagggcga gcgcgacggc accgaccgag tcgagaatct gcaggaactg
    24301 gtgaacgcgg cacaggtttt tgtgttcgac gagaacttcg acggcttgcc ggcggggcgt
    24361 gtcgttgacg c
//
LOCUS       DASTNK010000084        24266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_985759, whole genome shotgun sequence.
ACCESSION   DASTNK010000084 DASTNK010000000
VERSION     DASTNK010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 24266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24266
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985759"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /gene="murG"
                     /locus_tag="VFR20_06580"
     CDS             <1..957
                     /gene="murG"
                     /locus_tag="VFR20_06580"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEU0230218.1"
                     /translation="PERMEGRLVPEYGFEMEPLRFSGVRGKGWMALARLPLTLFSACW
                     QAFGALRKVRPDVVLGMGGYVAFPGGVMAAVRRIPLAIHEQNAVAGSANRWLARLAAK
                     TLSGFPDTLPGATTVGNPVRSAMLAVAPVAQRYGARRGPLRLLVVGGSLGAGILNTIV
                     PAALATLPEEARPVVTHQAGEQHLASLRQAYEQAGVRARCHAFIEDMAAAFSDADVVI
                     CRAGAMTVAEVAAVGVAALFVPLPHAIDDHQTANARYLGDGNGAWVRSQSEFTVEWLS
                     QWLARRTRGELQDVAGRAHGRAWPDATRRIADACEQLARGQA"
     gene            954..2360
                     /gene="murC"
                     /locus_tag="VFR20_06585"
     CDS             954..2360
                     /gene="murC"
                     /locus_tag="VFR20_06585"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU0230219.1"
                     /translation="MKHRIRHIHFVGIGGSGMSGIAEVLFNLGYGISGSDIQESAVTR
                     RLAGLGIRIAIGHAVENVKGAGAIVTSTAVSDDNPEVRAARAARIPVVPRALMLAELM
                     RLKRGIAVAGTHGKTTTTSLVATVLAAGGLDPTFVIGGRLTSAGANARLGQGEYIVVE
                     ADESDASFLNLLPVMAIVTNIDADHMGTYEHDLARLKSAFIEFTHKLPFYGSAVLCQD
                     DPNVREIIPFVSRPVTTYGMGGDALVRAVNVRSEGTRMCFDVERDVGPQSLPTLAICL
                     NLPGRHNVLNALAAIAVATELGVADDAIATSLTAFNGVGRRFTQVGDFAVPAAHGGGT
                     FRVVDDYGHHPVEMAATLAAARGAWPDRRIVLAFQPHRYTRTRDCFEDFVQVLGDADA
                     VLLTEVYPAGEPPLVAADGRALMRALRVAGRVEPLFVEDVADLPRAILDFAKADDVVV
                     VMGAGSISRVPAQIGELA"
     gene            2357..3313
                     /locus_tag="VFR20_06590"
     CDS             2357..3313
                     /locus_tag="VFR20_06590"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU0230220.1"
                     /translation="MSARFGRVGVLYGGSSAEREVSLMSGRGVHAALVEAGVDAHLFD
                     TGTQSLADLASAGFDRVFIALHGRYGEDGTLQGALELLRLPYTGSGPLASALAMDKIM
                     TKKVWTHHGLPTPLFAVLGGEADLVGLTDRLGLPLIMKPPHEGSTLGVAKVVDAEALH
                     EAFRLAAGYDTEVLAERFIDGRELTVPLLGKGKATRALPIIEIVAPGGNYDFEHKYVS
                     NDTQYICPADLPTELSREIMRLACRAYVALGCEGWGRIDLMLDRHGRPWLLELNTSPG
                     MTSHSLVPMGAKAAGMSYAELCVAILSEASCKVAASNVEGGR"
     gene            3316..4125
                     /locus_tag="VFR20_06595"
     CDS             3316..4125
                     /locus_tag="VFR20_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEU0230221.1"
                     /translation="MFNDARLTNFIANTLALLAVLALVAGVVVWIVQRPYFAISHVRI
                     EPMHGSTLHYVSAASVQATIAGRLAGNFFTIDLDKARQLLQSVPWVRRVRIQRVWPNT
                     LVVRIEEQQALALWNENQMINTWGEAFSANQGELPDNAHLPQLDGPASSERLVVQRYA
                     ELMRWFSPLDLDVRQVTLSPRYAWTVVLSDGVRLELGRDPAADTLDPHGRSGALPFAA
                     RIERFVDAWPKLEKKIDGRKISNADLRYANGFAISLAPLPPKPPRTKSTRK"
     gene            4133..5359
                     /gene="ftsA"
                     /locus_tag="VFR20_06600"
     CDS             4133..5359
                     /gene="ftsA"
                     /locus_tag="VFR20_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU0230222.1"
                     /translation="MTRDIKDLIVALDIGTSKVVAVVAEILPEGRFEVLGLGQHDSQG
                     MRKGVVVNIESTVNSIQRALEEAELMADCKIREVYTGIAGSHITSFNSSGMVAVKDKE
                     VTQADVARVIETAKAVNIPTDQQVLHVLTQEFIVDSQEDIREPIGMSALRLEVRVHIV
                     TGAVSAAQNIVKCVRRCGLEVQDLILQPLASSMACLTSDEKELGVVLVDIGGGTTDIA
                     IYTGGAIRHTAVVPIAGDQITSDIAAMLRTPTPDAEEIKLRFGIAKQVLASVDEQIEV
                     PGLGDRPNRQVKRQALGAVIEPRVEELFTFVQQVVRESGYEDLLASGVVLTGGTALMP
                     GIVELAEDVFLKPVRVGVPNYEGSLADVMHNPRFSTAVGLLSEARLQRARGRKVAQQK
                     GSVKTLMARMREWFLN"
     gene            5559..6737
                     /gene="ftsZ"
                     /locus_tag="VFR20_06605"
     CDS             5559..6737
                     /gene="ftsZ"
                     /locus_tag="VFR20_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU0230223.1"
                     /translation="MMNFEMLDSSTNGTVIKVVGVGGAGGNAVAHMIHSGVNGVDFIC
                     ANTDAQALVATEAPVQIRLGRTGLGAGAKPEQGRASAETAREEIRAALTGANMVFITA
                     GMGGGTGTGAGPVVAEVAKELGILTVGVVTKPFTFEGNRRLKMAEDGIAELSRHVHSL
                     IVVLNENLYELMDEEATQEDCFKAADDVLRNACAGIAEIINVEGNVNVDFEDVKTIMG
                     EQGQAMMGTSIAAGADRARVAAEHAIACPLLEGVDLHGARGMLVNITASRSLKMRETR
                     EIMDTIRSYAAEDATVIFGTAYDESMGENLRVTVVATGLGNAIARPQLVSNRAEARRT
                     GTDDFPMMVGHDFANSNSDVPAVIRKPRGGNATAQVRALETAGMDHFDIPAFLRKQAD
                     "
     gene            7043..7966
                     /gene="lpxC"
                     /locus_tag="VFR20_06610"
     CDS             7043..7966
                     /gene="lpxC"
                     /locus_tag="VFR20_06610"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEU0230224.1"
                     /translation="MLRQRTIQKTISTKGVGLHSGRRVEITLRPAAPDTGIVFHRIDL
                     PEVVDLPARADKVGDTRMASVLQNGKVRVSTVEHLMSALSGLGIDNLHIDLTAEEVPI
                     MDGSAGTFVYLLRSAGLEEQMAPKRFLRVLKPIEVREGEGSALKWARLEPHDGFSLSF
                     AIDFHHPAIDSTANFAEVDFARDSYVKEVARARTFGFVNEVEALRAAGLARGGSLDNA
                     IVMDEYRVLNSDGLRYDDEFVKHKILDAIGDLYLIGKPLVARYVACKSGHSLNNQLAR
                     ALLARQDAWELVTYESSATAPKALVNEWKFV"
     gene            complement(8023..8508)
                     /locus_tag="VFR20_06615"
     CDS             complement(8023..8508)
                     /locus_tag="VFR20_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEU0230225.1"
                     /translation="MRRATTRSASGTKHTTDLSALAWLGRDAQGASVLTTAKALLSAE
                     HDIQALLPPALARTCRVARLDRQQITLAVPSAAHAAKLRQMAPSIARALAGHGRNISE
                     VVVRVQAGLPQNGAKTPHQKDVIPLDDTALSAFRDLRERIAPSPLADAIARLLEHHRR
                     T"
     gene            8549..9484
                     /locus_tag="VFR20_06620"
     CDS             8549..9484
                     /locus_tag="VFR20_06620"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU0230226.1"
                     /translation="MSKQEFSDPSGAGMGQRLRVVGLVVAAMAVSAAAGAWVQAQRAH
                     AMSAPSLAQQRALGKQLSRDALYLRENMKLLAGRVGEMQARLIALDELGNRVAKAADV
                     AYTDPEIHASFRRSAMDDDVFEGGPGSAKALGDHIAYLEKQVADQGDRFAMLDLVLTK
                     RAGEQARLPTLMPVHYTYLSSPFGWRRDPVTGRRALHTGIDLAAPRGTPIRAASGGVV
                     VKAGYESGYGKMVEISHGNGLDTLYAHASKVDVKVGELVTKGQVIANVGESGRATGAH
                     LHFEVRMAGQPLDPTLFLGRGSVRRLLAVTASYRG"
     gene            9599..12358
                     /gene="secA"
                     /locus_tag="VFR20_06625"
     CDS             9599..12358
                     /gene="secA"
                     /locus_tag="VFR20_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU0230227.1"
                     /translation="MVSLLKKLIGSRNDRLLKQFRQQVAKINALEPEISKLDDTQLAA
                     KTDEFRQQYKDGKSLDALLPQAFAVVREASKRVFGMRHFDVQMLGAIALHNGKIAEMR
                     TGEGKTLMATLAVYLNALTGRGVHVVTVNDYLARRDADWMARLYNFLGLTVGVVVPQQ
                     ENDEKIAAYAADITYGTNNEYGFDYLRDNMEYRAEDRRQRELAYAIVDEVDSILIDEA
                     RTPLIISGQADDNTELYVRINAVPPMLTRMASEPKPQEPEPDGDFWVDEKGQQVYLSE
                     AGHIKAEEILGKIGLLPEGESLYNARNITLVHHLMVALRAHNLFFLDQHYVVQEGEVV
                     IVDEFTGRLMVGRRWSDGLHQAVEAKEGVKIQNENQTLASITFQNYFRMYDKLSGMTG
                     TADTEAYEFQEIYSLETMIIPTNRPMIRNDQNDQVFKTDHEKYNAILADIQDCYERRQ
                     PVLVGTTSIENSERLSAMLVKAKLPHEVLNAKQHAREAEIVADAGKPGAITIATNMAG
                     RGTDIVLGGRIENQIDDIRGDDSLDQAEKQARIDAVRQAWAPVNQAVKDAGGLRIIGT
                     ERHESRRIDNQLRGRAGRQGDPGSSRFYLSLEDSLMRIFAGDRVRAIMERLRLPEGEP
                     IEARMVSRSIESAQRKVESRNFDIRKQLLEYDDVSNDQRKVLYAQRNEVLESQDVSEV
                     IGNLRDATVTERFRAFIPEESMEEQWDVPGLQTSLESELQIVMPLVEMLDKEPDLTDE
                     DLHERVVAEGKRLYDAKVELVGRENWAPFERSMLLQSIDTHWRGHLLLLDHLRQGIHL
                     RGYAQVDPKQEYKREAFELFGSMLERIRDEVVKVLMTVQIQSAEEEMAEAEPVRLDNV
                     TYHHSDYDVALSGADPGLGEIATGASGASGAAAGGLAVPKVGRNDLCPCGSGKKYKHC
                     HGRLA"
     gene            complement(12402..13877)
                     /gene="mgtE"
                     /locus_tag="VFR20_06630"
     CDS             complement(12402..13877)
                     /gene="mgtE"
                     /locus_tag="VFR20_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEU0230228.1"
                     /translation="MQDHAKPEGSSITSPRRLDPEDAQLALQEIQAILRRHEVADAVA
                     ARQAAGTDAPHAFEGILPARPASRVSDIVNGLHPADIAFILESLPVGERQIIWHLVDP
                     AHDADVLLEIEDWARESLIESMDHRDLVAATGTMDADEIADLVADLPADVVAEVQRGL
                     TDEERAQLVEAMGYPEDSVGAIMDFDMVRVREDVSLEVVLRYLRRLHELPDHTDQVFV
                     VDRQDRLRGTLYLSTLLVSEPDVDVSEVMDTNVLTLNPLDSDADAAGAFERYDLVSAP
                     VTDEQGRLIGRVTISEVVDVIQEDSQEQALSRAGLQEEDIFAPVRVALRNRAPWLLVN
                     LCTASTASFVASRFEDTVSHIVILAFLMSIVAGIGGNSGNQTMTIIIRALAVGRVTGA
                     NTWRLVKREMAVTFLVGLFGSLVAGLFAWAISDSAAIAVVMMAAMIGNMLIGATLGVV
                     IPILRDRFGKDPAMGSSVLLTFATDSLGFLIFLSLATVFLL"
     gene            complement(13888..14232)
                     /locus_tag="VFR20_06635"
     CDS             complement(13888..14232)
                     /locus_tag="VFR20_06635"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEU0230229.1"
                     /translation="MPMPADANIHGDVFGGWIMSQVDIAGSVPATRRAAGRVATVAVN
                     AFTFKQPVFVGDLLSFYAEILKVGRTSITVLVEVYAERQRLEREVVKVTEATLTYVAT
                     DEQRQSRPLPPL"
     gene            complement(14324..15526)
                     /locus_tag="VFR20_06640"
     CDS             complement(14324..15526)
                     /locus_tag="VFR20_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEU0230230.1"
                     /translation="MKLVDSIVGWQADIAAIRRDIHAHPELAYEESRTADVIARQLQE
                     WGIDAHRGLGITGVVGIVRGRSDNGRAVGLRADIDALPMRELNTFAHASTNEGKMHAC
                     GHDGHTAMLLGAARYLSEHRDFDGTVYLIFQPAEEGFSGAKKMMDDGLFDLFPMEAVF
                     GMHNWPGMPVGTFGVRSGPMMGSSNTFEITIEGKGAHAAMPHLGFDPVMAAVQLAQSF
                     QTIITRNLPPIEPAVISITQIHTGSADNVIPNSAEMRGTVRTFSDDILDVIEKRMGEI
                     TELTCQAFDCKASFAFHRKYPPTVNHPDETAFCADVLKDIVGEEHVDQTVKPSMGSED
                     FAFMLKAKPGCYIWIGNGQGNHRDAGHGMGPCMLHNGSYDFNDDLIPLGATYWVRLAQ
                     RWLARGAA"
     gene            15659..17332
                     /locus_tag="VFR20_06645"
     CDS             15659..17332
                     /locus_tag="VFR20_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017685748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0230231.1"
                     /translation="MQRPWLSGYPEDVPAEIDIGAYDSLVDLLDRACHRHASRVALVS
                     LGCRLRYADLDRHADAVAAWLQSKGLPTGSRVALMMPNVMAYMVILLGVLRAGMTVVG
                     VNPLYRERELEHQLQDSQSQAIFILENFAATLDKVPATKRPPHVVLVSVGDLLGPKGW
                     LVNFVVRHLQRRVPPCRLDGVVRLGTVLSQGAKAPRRGVAVRMDDVAVLQYTGGTTGV
                     PKGAMLTHGNLVANVLQVEAVARPVLHDEAMTIMTALPLYHVFALMVCGLFAVHKGIS
                     SVLITDPRDLRTLVGAWRRDPPHIFPAVNTLFNGLANFPAFAKLDFSTLRLCFGGGAA
                     VQEAVADKWRRVTGRTIIEGYGLSETSPLVCVNPTNSESYSGDIGLPVPSTDIVMLDD
                     AGEVQPYGSPGELSVRGPQVMKGYWGCPEETAKVFSADGFFRTGDIGIMNEQGRVRVV
                     DRKKDLILVSGFNVYPAEIEGVVAAHPGVLECAAVGVPDARTGEAVRLYVVRKDPSLT
                     QEQVLSWCAERLTAYKRPHSCEFREALPKSNVGKILRRALRDEARAQQG"
     gene            complement(17344..18195)
                     /locus_tag="VFR20_06650"
     CDS             complement(17344..18195)
                     /locus_tag="VFR20_06650"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU0230232.1"
                     /translation="MHSPRLPLSDSLDAAVCAAHAAAAILQAHFSNRDDLVIDKKARN
                     DLVSQADREAEAAIIEILRERTPEFGIVAEETGGQPSGAATWYIDPLDGTTNFLHGIP
                     QYAVSIALIGHAGTLACDDAPLNRDTPVIGVIYDPNREELFTALYNVGAWLNEHRISC
                     SLTETLSESVLCTGFPFRDFSFADQYMPTLDEAIRTTRGIRRMGAAALDLAWTACGRY
                     DGYWEMGLAPWDVAAGTIIVREAGGVVEDMHHAEPWPLSGYVYAGNRHVAPALDALIR
                     PRLKPRH"
     gene            18416..20284
                     /gene="mnmD"
                     /locus_tag="VFR20_06655"
     CDS             18416..20284
                     /gene="mnmD"
                     /locus_tag="VFR20_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HEU0230233.1"
                     /translation="MANPYQPLEPARIVIAAGIPRSVVYDDVYHSASGAFGQAEHVFL
                     KGNGLPARWRGRAGFTVCETGFGLGRNFLALWRAWRDDPHRCNRLHVVSFELHPFSRP
                     DLAGLLLPGLAQPLRLLGETLVAAWPSLLPGLHRLEFESGAVTLTLAFGPIGRLARQI
                     AASVDAFFLDGFSPERNPEMWTPTLFGQMVRMANRGATAASWCCASHVRKALADAGFL
                     VEKAPGFGRKREMTVATLRSNLGRAIPSICSAPERVLIVGGGPAGAGMARSLALRGVA
                     SLVIDPAFEHGRGASHRGHLAAAVTPRIARDDEIGARLSRAGVARAMQRWAGLNVAAR
                     PVCCGTLEPAVSGRDAAERQLTLDTLRFPRDWVRWLAPADATRLAGFAIPHGGVFFAS
                     GLLAQPEPLLESLLTGAGIDCVATSVARLVRTDGGGWSALDERGSEIASAPVAIVANA
                     VQAKTLLASVPGAGRAQRVMSMQRFPGQVSYVAPGPTVPAARVIVAGDGYWLPPVRGL
                     GVAGSTYALRNDMAVVTQEGHKEISNRLATMLGARSSDILARLGTPDGWAGQRAVATR
                     RLPVIGRVAGVEGLWVACGYGSRGLTWSALAGDIVAAALTGEPLPLERALLDAVAP"
     gene            20439..22658
                     /locus_tag="VFR20_06660"
     CDS             20439..22658
                     /locus_tag="VFR20_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEU0230234.1"
                     /translation="MPPKSDFAHVTQLDTLQFLTAKSALADFAFGDDAGMLLRIEAHA
                     PGVYRLRCGLADRVRDEKPAARARALAEMLLARQEPVGELAVSSAIDAPEYGWRLAQG
                     DVALELKHSPLGLTLYRTGEPVLSFVELSCARDGRDDAEPTWHLGFELDVDDGLYGLG
                     ETRADLDRRGERLVSDRAADRALPLVWSPLGWGLYFNTLCRVAHDLGANDPDLYTVDV
                     RAPILDMFIFAGDPSEILNQYTALAGRAGQPGLWPMGVWLDQAPGQSINELLTQVERF
                     RAAEFPLDAVRLATPVVYGFQADKPIFEWNSERVPDARELMQRLAAQNIQFSAPSFPG
                     VLAGTRLFDEWEDRGWLLMADAGGAQVFPGSAVTAGRPFGLLDLTHKDVFNLWVERQR
                     QMVDEGMGAPACDACFDIPDDVTARGGETGAMLRGLYPLLARSALFQAVAGHKTPQEG
                     VICSTDLFPSAQRFAWQLGPRVTNDWAGFEQSLRAALAAGNSGITAQVHGVGCAEQPA
                     HAMTPELYIRWLAANVFSANFSFQGVPALLPGAFDGPTRELVRHWLQWRYRLIPYVLG
                     IIEDAVRSGLPVQRSMAQAFPADPMAQVWDTQYLFGPALLVAPVLHAGAETRVYLPAG
                     DAWWDLNTGWRYEGGTTWTVPAGLDTLPVFGREGHMLCLGPTAQHTGEFNSARILEEV
                     WMFGMPEHSPAVMRNKIRVMQMQGSSYIKGLEGLKILPSEGLEVKRRGAEVRISRIR"
     gene            complement(22707..23513)
                     /locus_tag="VFR20_06665"
     CDS             complement(22707..23513)
                     /locus_tag="VFR20_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU0230235.1"
                     /translation="MRLHILSDLHLSQQGLPLPRARADIVILAGDIARPAQAIAWARG
                     FTQPVLYVAGNHEFYGGNLSATVQSLKTLASGSSIHVLDREALILAGIRFLGATLWTD
                     FMLDGDGAPRTLAMAQASEFMRDFSRIESEERQGTPLSPDESTRVFARHRDWLVRKLA
                     EPFEGPTVVITHHAPSPRSIHPRFANSPLNNCFVSDMEWLMGADRAVLWVHGHTHDSF
                     DYVVRGTRVVCNPRGYAKNGVNENPAFDPEFVVEIGSGGHFAPGVTGATV"
     gene            23606..23681
                     /locus_tag="VFR20_06670"
     tRNA            23606..23681
                     /locus_tag="VFR20_06670"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23639..23641,aa:Lys,seq:ttt)
     gene            complement(23951..>24266)
                     /locus_tag="VFR20_06675"
     CDS             complement(23951..>24266)
                     /locus_tag="VFR20_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230236.1"
                     /translation="SPGIRAASFSVDNGSLNYQILVMQDKNKTATFQGSMELLAEGRY
                     TNGKRTTISLDPVAFSVQRYTHLIGTADLPAGFTPYVITIHVINQATKKISATRTIRV
                     SR"
BASE COUNT         4267 a         7186 c         8201 g         4612 t
ORIGIN      
        1 cccgagcgca tggaaggtcg gttggtgccc gaatacggtt tcgagatgga gcctctgcgt
       61 ttttccggtg tgcgcggcaa aggctggatg gcgttggcgc gcttgccgtt gacgctcttc
      121 agcgcgtgct ggcaggcatt cggggccttg cgcaaggtgc gccccgatgt tgtgctgggc
      181 atgggtggtt acgtggcctt tcccggcggc gtgatggcgg ccgtgcgtcg cataccgctg
      241 gcgattcatg aacagaacgc ggtggcgggg tcggccaatc gttggctcgc gcgtctggcg
      301 gcaaagacct tgtcgggatt cccggatacg cttcctggcg ccacgacggt cggcaacccg
      361 gtgcgttcgg ccatgctggc cgtggcgcca gtggcgcagc gctatggggc gcggcgcggg
      421 ccattgcgtc tgctggtggt gggcggcagc ctgggcgctg gaatactgaa caccatcgtt
      481 cccgcggcgc tcgcgacgct tcccgaagaa gcgaggccgg tcgtcacgca tcaggctggc
      541 gaacagcatc tggcctcgtt gcgccaggcg tacgaacagg ccggcgtgcg ggcgcggtgc
      601 cacgcattca tcgaggatat ggccgcggcc ttttccgatg ccgacgtcgt catctgccgg
      661 gccggcgcga tgacggttgc cgaagtggcc gcggtggggg tagccgcgtt gttcgtgcct
      721 ctgccgcacg cgatcgatga ccaccagacc gccaacgcgc gctatctggg tgatggaaac
      781 ggggcgtggg tgcggtcaca gtctgaattc accgtggaat ggctgtcgca atggctggcg
      841 cggcgcacac gcggggagtt gcaggacgtg gccgggcgtg ctcacgggcg tgcctggccg
      901 gatgcgaccc ggcgcatcgc cgatgcctgt gaacaattgg cgagggggca ggcatgaagc
      961 atcgtatccg gcatattcat ttcgtgggca taggcggttc cggcatgagc ggtatcgctg
     1021 aagtgctgtt caatcttgga tacggtatca gcggttccga cattcaggag tccgccgtga
     1081 cgcggcggct ggcggggctg ggtatccgca tcgccattgg ccacgcggtc gagaacgtca
     1141 agggcgcggg cgccattgtg acatccacgg cggtgtcgga cgacaatccg gaagtgcgcg
     1201 cggcccgggc ggcgcgcatt cccgtggtgc cgcgcgccct gatgcttgcc gagttgatgc
     1261 ggctcaagcg cggcatcgcc gtcgccggca cgcatggcaa gaccacgacg accagcctgg
     1321 tcgccaccgt gttggcggca ggagggctgg atcccacttt cgtcattggc ggccgcctga
     1381 cctcggcggg cgccaacgcc cgtctcgggc agggtgaata catcgtggtg gaggcagatg
     1441 agtccgacgc gtctttcctg aacctgctgc cggtcatggc catcgtcacc aatatcgacg
     1501 cggatcatat gggtacgtat gagcacgatc tggcacgcct gaaaagcgct ttcatcgaat
     1561 tcacgcacaa gcttccattc tatggcagcg cggtgttgtg ccaggatgat ccgaacgtgc
     1621 gcgaaatcat tccgttcgtt tcacgtccgg tgaccactta tggcatgggg ggcgatgcgc
     1681 tggtgcgggc agtgaacgtg cgcagcgaag gcacgcgcat gtgcttcgac gtcgaacgcg
     1741 atgtcgggcc gcaatccttg cccaccttgg cgatctgcct gaatctgcct gggcgtcaca
     1801 atgtgctcaa tgcgctcgcc gccatcgccg tggcgaccga actgggcgtg gcagacgacg
     1861 ccatcgccac atcgctgacc gcattcaacg gcgtggggcg gcgcttcacc caggtcggag
     1921 acttcgccgt tcccgccgca cacggtggcg gaacctttcg tgtggtggac gactatggcc
     1981 accatccggt ggaaatggcg gcgacgctgg ccgcggcgcg tggtgcctgg ccggatcgcc
     2041 gaatcgtgtt ggcgtttcag ccacatcgct acacacgcac ccgtgactgt ttcgaggatt
     2101 tcgtccaggt tctcggggat gccgatgcgg tgctcctgac cgaggtgtat ccggccggag
     2161 agccgcccct ggtggcggcc gacggccgcg cgctgatgcg cgcgttgcgc gtggccgggc
     2221 gggtcgaacc tttgttcgtc gaagacgtcg ccgacttgcc gcgcgccatc ctggatttcg
     2281 ccaaggcgga tgacgtggtg gtcgtcatgg gcgcgggatc catcagccgg gtgccggcgc
     2341 aaatcggaga actggcatga gcgcgcgatt cggccgggtc ggggtgttgt atggaggcag
     2401 ttccgccgag cgcgaggtct cgctcatgtc gggacgcggt gtgcatgccg cgctcgtcga
     2461 agcaggtgtc gatgcgcatc tgttcgatac cggaacgcag agcctggccg atctcgcctc
     2521 cgcgggattc gaccgggtgt tcatcgcgct gcatggccgg tatggcgaag acggcacgct
     2581 gcaaggcgcg ctggaactgc tccgactgcc ctataccggc agtggtccgt tggcgtccgc
     2641 gctggcgatg gacaagatca tgaccaagaa ggtctggacg caccatggct tgcccacgcc
     2701 gctgttcgcc gtcctgggcg gcgaggcgga cctcgttgga ctgacagacc ggctgggtct
     2761 gccgctcatc atgaagccgc cgcacgaggg ctcgacactc ggggtcgcca aggtggtgga
     2821 tgccgaggca ctgcacgaag cattccgtct cgctgccgga tacgacaccg aagtccttgc
     2881 cgaacgcttc atcgacggca gggaactcac cgtgccgctg ttgggcaagg gcaaggcgac
     2941 ccgggcgctg cccattatcg aaatcgtggc gcccggcggc aattacgact tcgagcacaa
     3001 gtatgtgtcc aatgacacac agtacatctg ccccgctgat cttccgaccg agctgagtcg
     3061 ggaaatcatg cggctcgctt gccgagccta tgtggcgctg ggctgcgagg gatgggggcg
     3121 gatcgacctg atgctggatc gccacggccg gccgtggctg ctggagttga atacgtctcc
     3181 cggcatgacc agccattccc tggtgcccat gggcgccaag gcggctggca tgagctacgc
     3241 cgaattgtgc gtcgccatcc tgtcggaggc gtcttgcaag gtggcggcct ccaacgtcga
     3301 gggggggcgg tagtcatgtt caacgacgcc cgcctgacga atttcatcgc caatacgctg
     3361 gctttgctgg ctgtgttggc gttggtcgcc ggcgtcgtcg tctggatcgt gcaacgtccg
     3421 tacttcgcga tttcgcatgt gcgtatcgag cccatgcatg gctccacgct gcattatgtc
     3481 tccgcggcga gcgtgcaggc gacgatcgcg gggcgcctgg ccggcaattt cttcaccata
     3541 gatctggaca aggcgcgcca gttgctgcag tccgtgccct gggtccggcg ggtgcggatc
     3601 cagcgtgtct ggccgaacac gctggtggtc cgaatcgagg agcagcaggc gctcgcgctc
     3661 tggaacgaga accagatgat caacacctgg ggcgaggcgt tcagcgccaa tcagggcgaa
     3721 ttgcccgaca acgcgcacct gccacagctc gatggcccgg cgagctccga gcggctggtc
     3781 gttcagcgct acgctgaact gatgcgctgg ttttcacccc ttgatctgga tgtgcggcag
     3841 gtgacgctca gcccgcgata cgcctggacc gtggtgttgt ccgacggggt gaggctggaa
     3901 ctgggccggg atcccgccgc cgacacgctt gacccgcatg gtcggtccgg cgccctgcct
     3961 ttcgccgcgc gcatagaacg cttcgtcgac gcctggccga aactcgaaaa gaagatcgat
     4021 ggacgcaaga tcagcaacgc cgatttacgt tacgccaatg ggttcgccat aagcctggct
     4081 ccgctgccac ccaaaccccc tagaaccaaa tccactagaa aatagaaaga ctatgacccg
     4141 tgacatcaag gatctgatcg ttgcattaga catcggcacc agcaaggtgg tggcggtggt
     4201 ggccgaaatc ctgcctgagg ggcgcttcga agtgctgggc ctgggtcagc atgactccca
     4261 gggtatgcgc aagggtgtcg tggtcaacat cgagtcgacg gtgaactcca tccagcgcgc
     4321 actcgaggaa gccgagctca tggccgactg caagatccgt gaggtgtata cggggatcgc
     4381 gggcagccac atcacgagct tcaactccag cggcatggtc gcggtcaagg acaaggaagt
     4441 cacgcaggcg gatgtcgcgc gtgtgatcga gaccgccaag gcggtcaaca tcccgaccga
     4501 tcagcaagtg ctgcatgtgt tgacccagga gttcattgtc gattcccagg aagacatccg
     4561 cgaaccgatc ggtatgagcg cgctgcgcct ggaagtgcga gtgcatatcg tgacgggggc
     4621 ggtgagtgcc gcgcagaaca tcgtcaagtg tgtgcggcgc tgcgggttgg aagtgcagga
     4681 tctcattctg cagccgctgg cttcgagcat ggcgtgcctg acatcggacg aaaaggagct
     4741 cggggtcgtg ctggtcgaca tcggcggcgg cacgacggac atcgccatct acaccggcgg
     4801 cgccattcgt cacaccgccg tggtgcccat cgccggtgac cagatcacga gtgacatcgc
     4861 cgccatgctg cgcaccccca cgccggacgc cgaggaaatc aagctgcgtt tcggcattgc
     4921 caagcaggtg ctggcgagcg tcgatgaaca gattgaagtg ccagggctgg gggatcgtcc
     4981 caatcgccag gtcaagcgcc aggcgctggg tgccgtgatc gaaccccgtg tcgaagagtt
     5041 gttcacgttc gtgcagcagg tcgtgcgcga atcgggttat gaggatctgc tggcatccgg
     5101 ggtcgttctg acgggcggca ccgcgttgat gccgggcatc gtcgaattgg ccgaggacgt
     5161 gttcctcaag ccggtgcgtg tcggcgtacc gaattatgaa ggcagtctgg ccgatgtcat
     5221 gcataatccg cggttttcca ctgcggtggg cctgttgtcc gaagcgcggc tgcagcgcgc
     5281 gcgcgggcgc aaggtggcgc aacagaaggg aagcgtcaag acgttgatgg cgcgcatgag
     5341 ggagtggttc ttgaattgaa ttcagccccc tggggggccg gatgggcagg cggaacaacc
     5401 tgctcggggc ggaggcttca aacgtggcgc gagtggatcg ttccgagggt ggatcgacca
     5461 gcaggctggc cctcttttga agcgactgcc gaaagtggtg ttggggaact tttgtcaatt
     5521 acttcgattt actaggaaat gtgagggagt caattatcat gatgaacttt gaaatgctcg
     5581 acagcagcac gaacgggacc gtgatcaagg tggtgggagt cggtggcgct ggcggcaacg
     5641 cggtggccca catgatccat tccggggtca acggcgttga tttcatctgc gccaacaccg
     5701 atgcgcaggc attggtcgcc accgaggcgc cggtgcagat ccgtctgggc cgcaccggcc
     5761 tgggcgcggg cgccaagccc gagcagggcc gagcgtccgc cgaaaccgcc cgcgaagaga
     5821 tccgcgcggc gctgaccggc gcgaacatgg tcttcatcac ggccggcatg ggtggcggca
     5881 cgggtacggg cgcggggccg gtggtggccg aggtggccaa ggagcttggc atcctgacgg
     5941 tgggcgtggt gaccaagccg ttcacgttcg aaggaaaccg gcgcctgaag atggcagagg
     6001 acggcatcgc cgaactgtcg cgacatgtgc attcgctcat cgtggtgctc aacgagaacc
     6061 tgtatgagct gatggatgaa gaggctacgc aggaagactg cttcaaggcc gccgacgatg
     6121 tcctgcgcaa tgcctgcgcc ggtatcgccg agatcatcaa cgtggaaggc aatgtgaacg
     6181 tcgacttcga ggacgtcaag accatcatgg gcgagcaggg ccaggccatg atggggacgt
     6241 ccatcgccgc gggggccgat cgggcccgcg tggccgccga gcacgccatc gcgtgcccgc
     6301 tgcttgaagg cgtggatctg cacggcgcgc gcggcatgtt ggtcaacatc accgccagcc
     6361 gctcgttgaa gatgcgggaa acgcgcgaga tcatggacac catccgcagc tacgcggccg
     6421 aggacgcgac cgtcatcttc ggcacggcct acgatgagtc catgggcgag aatctgcggg
     6481 tgacggtggt tgccacgggg ctgggaaacg cgatcgcgcg ccctcagctc gtgtcgaacc
     6541 gcgctgaagc ccggcgcacg ggaacggacg attttccgat gatggtgggt cacgacttcg
     6601 ccaactccaa ttcggatgtc ccggccgtca ttcgcaagcc acgcggtggc aatgccacgg
     6661 cgcaggtgcg tgcgctggaa accgctggca tggatcactt cgacatccct gccttcctgc
     6721 gcaagcaagc ggactgacgg tattgccgcc cccatggggc gggagcctct cgcgcggacg
     6781 accgattgcc ggacgtcctg taggaccgac tctctcacaa ttgagcaggg tgtcgtccgc
     6841 gggctctatc gacacaggcg tgccttccct tttccccggg ttgcgcacgc ctgcttgtta
     6901 cctgaaatac atcggaacat gattcagggg ttggggaggt tcttgtcaca aaaaagccat
     6961 gtaggctagc cgaaacccgt tacaatacgg agttacccga atgcccaatt gttgaaaaca
     7021 aggctggatc tcattctcaa ccatgctacg tcagcgcacc atacagaaaa cgatcagcac
     7081 caaaggcgtg gggctgcatt ccgggcgtcg ggtcgaaatc accctgcggc cggcggcgcc
     7141 tgatacgggc attgtttttc atcgcatcga tcttcccgag gtcgtcgact tgcccgcccg
     7201 ggcggacaag gttggcgaca cacgcatggc gtcggtgctg cagaacggca aggttcgtgt
     7261 ttccacggtg gaacacctga tgtcggcgct gtcgggcctg ggcatcgata acctgcacat
     7321 agacctcacc gcagaagaag tgcccatcat ggatggcagc gcggggacgt tcgtgtatct
     7381 gcttcggtcc gccggtctgg aagagcagat ggcgcccaag cggttccttc gggtgctcaa
     7441 gcccatcgag gtgcgtgaag gcgaaggctc ggccctgaaa tgggcccgtc ttgaacccca
     7501 tgacgggttt tccttgtcat tcgccattga ttttcatcac cccgccatcg actccaccgc
     7561 gaatttcgcc gaagtggatt tcgcccgcga ctcctatgtg aaggaagtcg cccgggcgcg
     7621 cacgttcggt ttcgtgaacg aagtcgaggc gctccgggcg gccggcttgg cccgcggagg
     7681 cagcctggac aatgccatcg tcatggatga ataccgggtg ttgaattccg atggcctgcg
     7741 ttatgacgac gaattcgtca aacacaagat tcttgatgcc atcggcgact tgtacctgat
     7801 cggcaaaccg ctggtggcgc gctatgtcgc ctgcaaatca gggcatagcc tcaataacca
     7861 gttggcgcgc gccttgcttg cgcggcagga cgcctgggaa ttggtcacct acgaatcaag
     7921 cgcgaccgcg cccaaagcgc tcgtcaacga gtggaagttc gtctgacggg ctgattcatt
     7981 cggcctgttg atattgtcga cgggccctgt ttcccgcacc tttcaggtgc gtcgatgatg
     8041 ttcaagaagc cgggcgatgg cgtcggccaa tgggctgggc gcgatgcgct cgcgcaaatc
     8101 ccgaaatgcg ctcagggcgg tgtcgtcgag tgggatgaca tctttctgat ggggtgtttt
     8161 tgccccgttt tgcggcaatc ccgcttgtac cctgaccacg acttcgctta tgttgcgccc
     8221 atgccccgcc agggcccgcg caatgctggg ggccatctgc cgtagcttgg cggcatgcgc
     8281 cgcgctggga accgcaagcg tgatctgctg tctgtcaaga cgggcgactc ggcaggtacg
     8341 cgccaacgcg ggtggcagca gcgcctgtat gtcgtgctcc gccgagagta gcgccttggc
     8401 cgtcgtgagc accgatgcgc cctgggcgtc ccggcccagc caggcgagcg ccgacaggtc
     8461 tgtcgtgtgt ttcgtgcctg aggcggaacg tgtcgtggca cggcgcatga attcaatacc
     8521 ctgaaatgaa ggaagttatg tgaaattgat gtcgaaacag gaattttccg acccctcggg
     8581 ggcgggcatg ggccagcgcc tacgggttgt cgggttggtg gtggccgcga tggctgtgtc
     8641 ggcagcggcg ggcgcatggg tgcaggcgca acgcgcccac gccatgtcgg ccccaagttt
     8701 ggcgcaacag cgcgcccttg gcaagcaatt gtcgcgcgat gccttgtacc tgcgtgagaa
     8761 catgaagttg ctggctggcc gggtcggcga gatgcaggct cggctcattg cgctcgacga
     8821 gcttggaaac cgggtggcca aggcggctga cgtcgcctat accgatcccg agatccacgc
     8881 cagcttccgg cgatccgcca tggacgacga cgtgtttgaa ggcgggccgg gcagcgcgaa
     8941 ggcgctgggt gaccacatcg cttatctgga aaagcaggtg gctgatcagg gcgatcgttt
     9001 cgccatgctg gatctggtcc tgacgaaacg cgccggggag caagcccgct tgcccacgtt
     9061 gatgccggtc cattacacgt atctcagttc cccgttcgga tggcgccgtg acccggtcac
     9121 cgggcgccgg gccctgcaca cgggtattga tctggccgcg cccaggggta cgccgatcag
     9181 ggcggcgtcg ggcggtgtgg tcgtcaaagc gggctacgag tcgggctacg gcaagatggt
     9241 cgagatcagc catggcaatg gcttggacac gctctatgct catgcctcga aggtggatgt
     9301 gaaagtgggc gaactggtca ccaaggggca ggtgatcgcc aatgtggggg aatcgggccg
     9361 cgccaccgga gcccacctgc atttcgaagt gcgcatggcc ggacagccgc tggatccgac
     9421 cctgttcctg gggcgggggt ctgtgcgtcg cttgctcgcc gtgaccgcct cgtacagggg
     9481 ctgacgtttt gttgcgaaag gtgcgttaaa ctgtctcgtt tgcctttcgc gttttatcgc
     9541 taacctgaac agagagattg agacctggtc atgccgggtc cgcatcgaca cgacaagaat
     9601 ggtatccctg ctaaagaagc tgattggtag ccgcaacgac cgcctattga aacaatttcg
     9661 ccaacaagtg gcgaaaatca acgctctcga gccggagatc tccaaactcg acgatacgca
     9721 gcttgccgcg aagaccgatg aattccggca gcaatacaag gacgggaaat cgctcgatgc
     9781 gctgctgccc caggccttcg ccgtggtgcg cgaggccagc aagcgcgtct tcggcatgcg
     9841 ccacttcgac gtccagatgc tgggcgccat cgcgttgcat aacgggaaaa tcgccgaaat
     9901 gcgcacgggt gagggcaaga ccctcatggc cacgctcgcg gtgtacttga atgccctgac
     9961 ggggcgtggc gtgcacgtgg tcaccgtcaa tgactacctc gcccgtcgcg acgccgactg
    10021 gatggcgcgc ctgtacaact tcctgggcct gacggtgggg gtcgtggttc cgcagcagga
    10081 aaacgacgaa aaaatcgcgg cctacgcggc cgatatcacc tacggcacga acaacgaata
    10141 cggcttcgac tatctgcgcg acaacatgga gtaccgggcc gaggatcgcc gtcagcgcga
    10201 actggcctat gccatagtcg acgaggttga ctccattctc atcgacgagg cccgcacacc
    10261 gctgatcatc tccggccagg ccgacgacaa caccgagctg tacgtgcgca tcaacgccgt
    10321 gccgcccatg ctcacgcgca tggcctccga gcccaagccc caagagcctg aaccagacgg
    10381 cgatttctgg gtcgatgaaa aaggccagca ggtgtatttg tccgaggccg gccacatcaa
    10441 ggccgaagaa atccttggca agatcggcct gctgcccgag ggtgagtcgc tgtacaacgc
    10501 gcgcaacatc acgctggtgc accacctgat ggtggccctg cgggcgcaca acctgttttt
    10561 ccttgatcag cattacgtgg tgcaggaagg cgaggtcgtc atcgtcgacg agttcacggg
    10621 gcggttgatg gtcggacgcc gctggtccga cggcctgcat caggcggtcg aggccaagga
    10681 aggcgtcaag atccagaacg agaaccagac gctggcatcc atcaccttcc agaactattt
    10741 ccgcatgtac gacaaactgt cgggcatgac cggaacggcc gataccgagg cgtatgagtt
    10801 ccaggagatc tattccctgg aaaccatgat catcccgacg aatcgtccca tgatccggaa
    10861 cgaccagaat gatcaggtct tcaagaccga tcacgagaag tacaacgcca ttctcgccga
    10921 catccaggat tgttacgagc gtcggcaacc cgtgctggtg ggcaccacca gcatcgagaa
    10981 ctcggaacgc ctgtcggcca tgctggtgaa ggccaaactg ccgcacgagg tgctcaacgc
    11041 caagcagcat gcgcgcgaag ccgagatcgt cgccgacgcc ggcaagcccg gcgccatcac
    11101 catcgccacc aacatggcgg gccgcggcac cgatatcgtg ctcggcggtc gcatcgagaa
    11161 ccagatcgac gacattcgtg gcgacgattc gctggaccag gcggaaaagc aagcccgtat
    11221 cgatgccgtg aggcaagcct gggcgccggt caatcaggcg gtcaaggatg cgggcggtct
    11281 gcgtatcatc ggcaccgagc gccacgagtc gcggcgtatc gacaaccagt tgcgcggccg
    11341 cgcgggccgc cagggtgatc cgggttcgtc gcgcttctac ctgtcgctgg aagactcgct
    11401 catgcgcatc ttcgcgggcg atcgtgtgcg tgccatcatg gagcgcttgc gtctgcccga
    11461 gggcgagccc atcgaggccc gcatggtgtc gcgttccatt gaatcggcgc aacgcaaggt
    11521 cgagagccgg aatttcgaca tacgcaagca attgctcgaa tacgacgatg tctccaacga
    11581 ccagcgcaaa gtgctgtacg cacagcgcaa cgaagtgctg gaatcgcaag atgtctcaga
    11641 ggtcatcggc aatctgcgcg acgccaccgt gactgaacgc ttccgtgcct tcattcctga
    11701 agaatccatg gaagagcagt gggacgtccc ggggttgcag acctccctgg aatccgagtt
    11761 gcagatcgtg atgccgctgg tcgagatgct cgacaaagag cccgatctca ccgacgagga
    11821 cctgcacgaa cgagtcgtgg ccgagggcaa gcgcctatac gacgcaaagg tcgaactggt
    11881 gggccgcgag aattgggcgc cgttcgaacg ttcaatgctg ctgcagtcca tcgatacgca
    11941 ttggcgcggc cacctgttgc tgcttgacca tctgcgccag ggtattcatc tgcgtggcta
    12001 tgcccaggtc gatcccaagc aggaatacaa gcgcgaggcg ttcgaattgt tcggcagcat
    12061 gcttgaacgc attcgcgacg aagtggtcaa ggtgctgatg acggtgcaaa tccagtcggc
    12121 cgaggaggaa atggccgagg ccgagccggt gcgtctggac aacgtcacgt accatcattc
    12181 cgactatgat gtggcgctca gcggtgccga ccccggcctc ggcgagatcg ccaccggcgc
    12241 ctcgggcgcc tcgggcgcgg cggccggcgg gctggccgtg cccaaggtcg ggcgcaacga
    12301 tttgtgtcct tgcggcagcg gcaagaagta caagcactgc cacgggcgcc tggcctgatc
    12361 tcccatggca cgggtccggg tccgtgccat cgaagcccgc gtcacagcag gaacacggtc
    12421 gccagactca gaaaaatcag gaaacccagg gaatcggtgg cgaaagtcag cagcaccgac
    12481 gaccccatcg cggggtcttt cccgaagcgg tcgcgcaaaa tggggatgac caccccgagc
    12541 gtggcgccga tcagcatgtt gccgatcatc gccgccatca tgaccacggc aatggccgcc
    12601 gagtcggaaa tggcccaggc gaacagaccg gcgaccaggc tgccgaacag ccccaccagg
    12661 aacgtcacgg ccatctcccg tttgaccagc cgccaggtgt tggcgccggt gacgcgcccc
    12721 acggccagcg cccggatgat gatggtcatt gtttgattgc ccgagttgcc gccaatcccc
    12781 gcgacgatgg acatcaggaa cgccaggatg acgatatggc tgacggtgtc ttcgaagcgc
    12841 gacgccacga acgaggcggt cgaggcggtg cacaagttga ccaacagcca cggggcgcgg
    12901 ttgcgcaagg ccacccgcac aggggcgaaa atatcctctt cctgcaagcc ggcgcgtgac
    12961 agggcctgtt cctgcgagtc ttcctggatg acatcgacga cttccgaaat ggtcacccgt
    13021 ccgatcagcc gcccctgctc atcggtcacc ggggcggaga ccagatcgta gcgctcgaag
    13081 gcccccgccg cgtcggcgtc ggaatccagg gggttcagtg tcaggacatt ggtgtccatc
    13141 acttccgaga cgtcgacgtc gggttcgctg accagcaacg tcgacagata cagcgtgccg
    13201 cgcagcctgt cctggcggtc caccacgaac acctggtcgg tgtggtcggg cagttcgtgc
    13261 aggcgccgca ggtatcgcag cacgacctct agagagacgt cctcccgcac ccggaccatg
    13321 tcgaagtcca tgatggcgcc cacggagtcc tcggggtagc ccatggcttc gacgagttgc
    13381 gcgcgctcct cgtcggtgag accacgctgg acttcggcca ccacgtcggc aggaagatcg
    13441 gccaccagat cggcgatctc gtcggcatcc atcgtgccgg tcgccgcgac caggtcccga
    13501 tgatccatcg actcgatgag tgactcgcgc gcccaatcct cgatctcgag cagcacatcg
    13561 gcatcgtgcg cggggtcgac caaatgccag atgatctgcc gttcgccgac cggcagggat
    13621 tccaagatga aggcgatatc ggccggatgc aggccgttga cgatgtcgct gaccctggag
    13681 gctggccgcg ccggcaggat gccctcgaag gcgtgcggag cgtcagtgcc ggcggcttgg
    13741 cgcgccgcca cggcgtcggc gacttcgtgg cggcgcagga tggcctggat ctcttgcagc
    13801 gcgagttggg cgtcttcggg gtccagtcgg cgggggctgg tgatagacga gccttccggc
    13861 ttggcgtgat cctgcatggc cccgttgtca gagaggcggc agcggacggc tctggcgttg
    13921 ttcgtccgtg gcgacgtaag tcagggtggc ttccgtgacc ttgaccacct cgcgctccag
    13981 gcgctggcgc tcggcataga cctcgaccag cacggtgatg gacgtgcggc cgactttgag
    14041 gatctcggcg tagaagctca gcaggtctcc cacgaaaacg ggttgcttga acgtgaaggc
    14101 gttgacggcc acggtggcga cccggccggc ggcgcgccgg gtcgcgggca cggagccggc
    14161 gatgtcgacc tgcgacatga tccagccgcc gaagacgtct ccgtgtatgt tggcgtcggc
    14221 cggcatgggc atcacgcgca gcacggcgtg tcgttcgggc gggggaaggg tggaggatga
    14281 ggaagcgggg gtcacagtga aggttcctgg agaataggtt tggtcaggcc gcgccgcgcg
    14341 cgagccagcg ctgggccagt cgcacccagt aggtggcgcc aagcggaatc aggtcgtcgt
    14401 tgaagtcgta gctgccgtta tgcagcatgc aggggcccat gccgtgcccc gcatcgcggt
    14461 ggttgccctg gccgttgcct atccagatgt agcaccccgg tttggccttg agcatgaagg
    14521 cgaaatcctc ggagcccatg gacggcttga cggtctggtc gacgtgttcc tcgcccacga
    14581 tgtccttgag cacgtcggcg caaaacgcgg tttcgtcagg atggttgacc gtgggcggat
    14641 atttccggtg aaaggcgaag ctggccttgc agtcgaatgc ctgacaggtg agttcggtga
    14701 tttcgcccat gcgcttctcg atgacgtcca ggatgtcgtc ggagaaggtg cggacggtgc
    14761 cccgcatttc ggcgctgttg gggatcacgt tgtcggccga acccgtgtgg atctgcgtga
    14821 tgctgatcac cgcgggctcg atgggaggga ggttgcgcgt gatgatggtc tggaatgact
    14881 gcgcaagctg cacggcggcc atgaccgggt cgaaacccag atggggcatg gcggcgtgcg
    14941 cccccttgcc ttctatcgtg atttcgaatg tgttgctcga gcccatcatg gggcccgacc
    15001 gcacaccgaa cgtgcccacc ggcatgccgg gccagttgtg catgccgaac accgcctcca
    15061 ttggaaacag gtcgaagagg ccgtcgtcca tcatcttctt ggcgccgctg aacccctctt
    15121 cggcgggctg gaagatcagg tagacagtgc catcgaaatc gcggtgctct gacaggtaac
    15181 gcgccgcgcc cagcagcatg gccgtgtggc cgtcatggcc gcaggcgtgc attttgcctt
    15241 cgttggtgct ggcgtgtgcg aatgtgttga gttccctcat cggcagcgcg tcgatgtcgg
    15301 cgcgcaggcc caccgcacgg ccgttgtctg accggccccg cacgatgccc accacgccgg
    15361 tgataccaag ccctcgatgc gcgtcgatgc cccattcctg caattgcctc gcaatgacat
    15421 ccgccgtgcg gctttcttcg taggccagtt cgggatgggc gtggatgtcg cggcggattg
    15481 cggcgatgtc cgcctgccag ccaacaatgg aatcgactaa tttcatggtg ggcgatcctg
    15541 taagatatgt agtcgggatg atacgtgata taccgatccg acgctgcccg ggcctgcacg
    15601 actatcctgg cctcggcggt atcatcgtcc tgacgccaat catacaaggg ttcggatcat
    15661 gcagcggccg tggctttctg gataccccga ggacgtgccc gccgaaatcg acatcggcgc
    15721 ttatgactcc ctggtcgatt tgcttgaccg ggcctgtcac cggcacgcgt cccgggtcgc
    15781 gctggtgtca ctggggtgcc gcctgcgtta tgccgacctc gatcgccacg ccgacgcggt
    15841 ggccgcctgg ctgcagtcca aggggctgcc gactgggagc cgcgtggcgt tgatgatgcc
    15901 caacgtgatg gcgtacatgg tcatcctgct tggggtgctg cgcgcgggca tgacggtggt
    15961 gggggtcaac cccctctatc gcgaacgcga actcgagcat cagttgcaag acagccagtc
    16021 ccaggcgatc ttcatcctcg agaatttcgc ggcgactctg gataaagtcc ccgccaccaa
    16081 acggcccccg catgtggtgc tggtatcggt gggagatctg ctgggcccaa agggttggct
    16141 ggtgaatttt gtcgtacgcc acctacagcg acgcgtcccg ccttgtcgtt tggatggcgt
    16201 ggtgcgtctc ggcaccgtat tgagccaggg ggcgaaggcg ccgcggcgcg gcgtcgcggt
    16261 ccgcatggat gatgtggcgg tgctgcaata cacgggcggg acgaccggcg tgccgaaggg
    16321 ggccatgctg acgcacggca acctggtcgc caatgtcctg caggtcgagg cggtggcgcg
    16381 gccggtattg catgacgagg ccatgaccat catgaccgcc ttgccgctct atcatgtgtt
    16441 cgccctgatg gtgtgcgggt tgtttgccgt gcacaagggc ataagcagtg tgctcatcac
    16501 cgatccgcgc gacttgcgta ccttggtcgg tgcttggcgg cgtgacccac cgcatatctt
    16561 tccggctgtc aataccttgt tcaacgggct ggccaatttc ccggcgttcg cgaagctgga
    16621 cttttcgact ctgcgactat gcttcggtgg cggtgccgcc gtgcaggaag ctgttgccga
    16681 caagtggcga cgcgtcacgg gccgtaccat catcgaaggc tacggcctgt cggaaacctc
    16741 gcccctcgtg tgcgtcaatc ctacgaattc cgagtcatat tccggtgaca tcgggctgcc
    16801 tgtgccgtcg accgacattg tcatgcttga tgatgcaggc gaggtacaac cctatggctc
    16861 tcccggggaa ctgtccgtgc gcgggccgca ggtcatgaaa ggctactggg gttgcccaga
    16921 ggaaaccgcc aaggtgtttt ccgctgacgg ttttttccgc actggcgaca tcggcatcat
    16981 gaatgaacag ggtcgtgtgc gcgtcgtcga ccgcaagaaa gatctgatcc tggtgtccgg
    17041 cttcaacgtc taccccgccg agatcgaggg ggtggtggcg gcacaccccg gggtgctcga
    17101 gtgcgccgcg gtgggggtgc ccgatgcgcg cacgggcgag gcggtgcgcc tttacgtggt
    17161 caggaaagat ccatcgctca cgcaagaaca ggtgctgtca tggtgcgccg aacggctcac
    17221 cgcatacaag cgcccgcatt cttgcgagtt ccgcgaggcc ttgcccaaga gcaacgtggg
    17281 caagatactg cgccgggcgt tgcgcgatga agcgcgggca cagcagggct gacgcttggt
    17341 ggttcagtgt cgcggcttga gcctggggcg aatcagggcg tcgagcgccg gtgcgacatg
    17401 ccgattgccg gcataaacgt aaccgctgag cggccagggt tcggcgtgat gcatgtcttc
    17461 gaccaccccc ccggcttcgc gcacgatgat ggtcccggcc gcgacatccc agggggccag
    17521 acccatttcc caataaccgt cgtagcggcc gcaggcggtc catgccaggt cgagtgccgc
    17581 cgcgcccatg cggcggatgc cgcgagtggt cctgatggct tcgtcgagcg tgggcatgta
    17641 ttggtcggcg aaggagaaat cgcggaatgg gaaaccggtg cacaggaccg attcgctcag
    17701 ggtttccgtg agcgagcaac tgatgcgatg ttcgttgagc caggccccga cgttataaag
    17761 tgcggtgaag agctcttcgc gattagggtc gtagatgaca ccgatgacag gagtgtcgcg
    17821 gttgagcggc gcgtcatcac aggcaagggt tccggcgtgg ccgatgaggg cgatggagac
    17881 ggcgtactgc gggatgccgt gaagaaaatt ggtcgtgccg tcgagcgggt ctatgtacca
    17941 ggtggcggcg ccagagggct gtccgccggt ttcctcggcc acgataccga attcgggcgt
    18001 gcgttcgcgc aggatctcga tgatggccgc ctcggcttca cgatccgctt gtgagaccag
    18061 gtcgttacgg gctttcttgt ctatgacgag gtcgtccctg ttggaaaaat gcgcttgcag
    18121 aatcgccgcc gccgcatgcg cggcgcagac cgcggcgtcc aaggaatcgc taagggggag
    18181 tcgtggagaa tgcataccgt cgctgaagct gggttgaggg acgtggggtg gattcgttgc
    18241 gtgaccgcga cgccccgctc gttggttatt cttagtgtac gcaacattgg cgggatcgtc
    18301 ccgccaatgg tgccataatg gccgcgccgg tcggcgccac cacgtggagt tgttgttttt
    18361 tcgacggccg tggcattgcg ccccagggcg gtgccccgat tcaaacctgg acatcatggc
    18421 caatccgtat caaccgctgg agccagcccg catcgtcatc gctgcgggga taccccgaag
    18481 cgtggtctac gatgacgtct atcattccgc gtcaggagcc tttgggcagg cagagcacgt
    18541 gtttctgaaa ggcaatgggt tgccggcccg gtggcgtggg cgagccgggt tcaccgtctg
    18601 tgaaacggga ttcggcctgg ggcgcaactt tctcgcgctc tggcgggcat ggcgcgatga
    18661 tccgcatcgt tgcaaccgtt tgcacgtggt ctctttcgag ctgcatccgt tttcccgccc
    18721 tgatttggcc ggcctgctcc tgcccgggct tgcgcagcca ctgagattgt tgggtgagac
    18781 gctggtcgcc gcatggccat cattgttgcc cggcttgcat cggctggaat tcgaatcagg
    18841 agcggtgaca ttgacgctgg ctttcgggcc gatcggtcgt ctggcccgac agatcgcggc
    18901 atcggtcgat gcgttctttc tggatggctt ctctccggag aggaatccgg agatgtggac
    18961 gccgaccttg ttcggccaga tggtgcgcat ggccaatcgc ggcgcgacgg cggcaagctg
    19021 gtgttgcgca tcgcatgtgc gcaaggcctt ggcggatgcc ggctttctgg tcgagaaggc
    19081 gcccggcttt gggcgcaagc gcgagatgac cgtggcgacg ctgcgatcga atctgggtcg
    19141 tgcgattccg tcgatctgtt cggcgccgga gcgcgtgctg attgtgggtg gagggccggc
    19201 gggtgcgggc atggcgcgga gtctggcgct tcggggcgtg gcgtccttgg tgatcgaccc
    19261 cgcgttcgaa cacgggcgag gggccagtca ccggggtcac ctggcggcgg cggtgacgcc
    19321 acgcattgcg cgcgacgatg aaatcggggc acgcctgtcg cgtgcgggcg tggcccgcgc
    19381 catgcagcgc tgggccgggc tgaacgtggc cgcccggccg gtgtgctgcg gtacgctgga
    19441 gccggcggtc tcgggccgtg acgcggcgga gcgccaactc accctggata cgttgcggtt
    19501 ccctcgggat tgggtgcgtt ggttggcgcc tgcggacgcg actcgtctgg cgggtttcgc
    19561 cattccgcac ggtggtgtgt tctttgccag cgggctgctc gcgcagcccg aacctttgct
    19621 tgagtccctg ctcacagggg ccggcatcga ttgcgtcgcc acaagcgtgg cgcgccttgt
    19681 ccggacggac gggggaggtt ggtcggcgct ggatgaacgc ggttcggaga tcgcctcggc
    19741 gccggtcgcg atagtggcca atgccgtcca ggccaagacg ttgttggcca gtgtgcctgg
    19801 cgccgggcgt gcccaacgcg tgatgtcgat gcagcgtttt ccggggcagg tgagctatgt
    19861 agcccccggc cccaccgtgc cggctgcgcg ggtcattgtc gccggagacg gctattggtt
    19921 gccgcccgtg cggggattgg gtgtggcggg gagcacgtac gcgctgcgga atgacatggc
    19981 ggtggtgacc caggaagggc acaaggagat ctcaaatcgg ctcgcgacca tgctgggtgc
    20041 acggtcgtca gacattctgg ctcgtcttgg aacaccggat ggttgggcgg ggcagcgcgc
    20101 cgtggcgacc cggcgcttgc cggtcatagg cagggtggcg ggcgttgaag ggctgtgggt
    20161 ggcctgcggg tatggttcgc gagggttgac ctggtcggcc ctggccggtg acatcgtggc
    20221 ggcggcattg accggtgagc cgctgccgtt ggagagggcg ttgctggacg ccgtggcgcc
    20281 gtagatcgtg aatgattctc cccaaaccgc gcgatgtgcg gttgcctgga tggggggtgg
    20341 cgacaagatt tagcggacgc gattttattt tatagcgcaa atccgtcaga atactaactt
    20401 ttgaatgttt tttgggctaa agccccaagg attgcgctgt gccgcccaag tctgattttg
    20461 ctcatgtgac gcaactggat acattgcagt ttctgacagc caaatctgcg cttgcggatt
    20521 tcgcgttcgg tgacgatgcc ggcatgttgc tgcgcatcga ggcgcatgcg ccaggcgtat
    20581 atcgtctgcg gtgcgggttg gccgaccggg tgcgagacga aaagcccgcc gcccgggccc
    20641 gcgcgctggc cgagatgctg ctcgcgcgcc aagagcctgt cggtgagctc gctgtcagtt
    20701 ccgccatcga cgcgccggaa tacggctggc gccttgcgca gggcgacgtt gccctagagt
    20761 tgaagcattc gccgctgggc ctcactttgt atcgcaccgg cgagcccgtg ctgagttttg
    20821 tcgaattgtc ctgcgcgcgc gatgggcggg atgatgccga gccaacctgg catctgggtt
    20881 tcgaactcga tgtggacgat ggtctgtacg ggcttggcga aactcgggct gatcttgacc
    20941 gtcgcggtga gcgcctggtt tccgatcgag cagccgaccg ggcgcttccc ctggtgtgga
    21001 gcccacttgg gtgggggctc tatttcaata cgttgtgccg agtggcgcac gacctgggcg
    21061 cgaacgatcc ggacctctat accgtcgacg tgcgcgcgcc gatactcgac atgttcatct
    21121 tcgcgggtga tccctccgag atactgaatc aatacacggc gctcgcgggc cgggcggggc
    21181 aaccggggtt gtggcccatg ggggtctggt tggaccaggc gccggggcaa tccatcaatg
    21241 aattgctgac ccaggtcgaa cggttccgcg cggccgaatt tccgctcgac gcggttcggc
    21301 tcgcaacacc ggttgtctat gggttccagg cggataagcc gatattcgag tggaacagcg
    21361 aacgggtgcc cgatgcgcgt gagctcatgc aacgtctggc cgcccagaac atccagttct
    21421 cggccccttc atttccgggg gtcctggctg gcacgcggct gttcgacgaa tgggaggatc
    21481 gaggctggct gctcatggcg gacgcgggcg gcgcccaggt gtttccgggt agcgcggtga
    21541 ccgccgggcg gccattcggg ttgctcgatc tgacgcacaa ggatgtcttc aacctgtggg
    21601 tggagcgtca acggcaaatg gttgacgagg gtatgggtgc gccggcgtgt gacgcctgtt
    21661 tcgacattcc cgacgatgtc accgcgcgcg gtggcgaaac gggtgccatg ctgcgcgggc
    21721 tctatccgct gctcgcccgg agcgccttgt tccaggccgt ggccggccac aagacgccgc
    21781 aggagggtgt gatctgcagc actgatttgt tcccatcggc gcagcgtttc gcttggcaac
    21841 tcggcccccg ggtgacgaac gactgggccg gcttcgagca gtcgctgcgc gccgcgttgg
    21901 cggcgggcaa cagtggcatc acggcgcaag tgcatggcgt gggctgcgca gagcaacccg
    21961 cgcatgccat gacgccggaa ctctatatcc gctggctggc cgccaacgtg ttctcggcca
    22021 acttcagctt ccagggggtg ccggccctgc tgcccggggc tttcgatggc ccaacgcgtg
    22081 aactcgtgcg ccattggctg cagtggcgct atcggttgat tccctacgtg ctgggcatca
    22141 tcgaagatgc cgtgcgcagc gggctgccgg tgcagcgttc catggctcag gcttttcccg
    22201 ccgaccccat ggcccaggtg tgggatacac aatatctatt cgggccggcg cttctggtcg
    22261 cgccggtgct gcacgccggt gccgagaccc gggtctatct gccggcgggt gatgcctggt
    22321 gggatctcaa cacgggctgg cgctatgaag gcggcaccac ctggactgtt ccggctgggc
    22381 tggatacctt gccggtgttc gggcgcgaag gccacatgct ctgcctgggg ccgaccgctc
    22441 aacataccgg ggaattcaat tcggcccgca tcctcgagga agtctggatg ttcggcatgc
    22501 ccgagcacag cccggcggtc atgcgcaaca agatccgggt catgcagatg cagggctcca
    22561 gctatatcaa ggggctggaa ggcttgaaga tcctgccttc cgaggggctg gaggtgaaac
    22621 gccgtggcgc cgaggtgcgc atttcgcgca tacgctgagc ctggttccag agttcttttg
    22681 atcatctagg tccaggatcc ctaaggctaa accgtggcgc cagtcacgcc cggcgcgaaa
    22741 tggccgcctg aaccaatttc cacgacaaat tccggatcga acgcgggatt ctcgttgacg
    22801 ccgttcttcg catagccccg ggggttgcat acgacccggg tgccacgcac cacatagtcg
    22861 aagctgtcgt gcgtgtgccc atgcacccat aggaccgccc ggtccgctcc catcagccat
    22921 tccatatccg acacgaagca gttgttcagc ggcgagttcg cgaaccgtgg atggatgctg
    22981 cgcggagagg gggcgtgatg cgtgatgacc actgtggggc cttcgaacgg ttcggcgagt
    23041 tttcggacca gccagtctcg gtggcgcgcg aagacgcggg tggattcgtc gggcgaaaga
    23101 ggtgtgcctt gccgctcttc ggattcgatc cggctgaagt cccgcatgaa ttccgatgcc
    23161 tgagccatgg ccagtgtgcg cggcgcaccg tctccatcca gcatgaaatc cgtccagagc
    23221 gtggcgccca ggaaacgtat gcctgccagg atcagggctt cccgatccaa cacatggatg
    23281 gaggatccgc ttgccagtgt tttcaggctc tgcaccgtgg ccgacagatt tccgccgtag
    23341 aactcatgat tgccggctac gtacaggacg ggctgcgtga agcctctggc ccaagcgatg
    23401 gcttgtgcgg gacgggcgat gtcgccggcc agaatgacga tgtcggcccg ggcccgcggc
    23461 agtggcagcc cctgctggct gaggtggagg tctgaaagaa tatgcaggcg aatgggggat
    23521 tcgatcatag gcgccgtact atcaatcaca ggttccgcaa tttgacaggg gaagggaatg
    23581 gccccataat ctcgctcttt cctttgggtt ggtagctcag ctggtagagc accggacttt
    23641 taatccgttg gtcccgggtt cgagccccgg tcaacccacc agtaaaatca agcgtgcggc
    23701 gaccaggtca aacgcataga aatgtgcata gtacaggtgc atagtacagg tgcatagtac
    23761 aggcagcgca cggtaaagca ccacagtgcc tgcgcacagc cggcgggtgc atggttggca
    23821 ggcacatggc cgacaggtgc gtggcaacca cgtagattag caccgcccaa gcagcgccaa
    23881 tagcccgcag tgcaaatgcc attgagcccg accatgaaaa aacgccgcgt catgcggcgt
    23941 ttttgtttga tcagcgtgat acccggatcg tgcgcgttgc gctgattttc ttcgttgcct
    24001 ggttgatgac gtggatggtg atcacgtaag gcgtgaaacc cgccggtaga tcggccgtgc
    24061 cgatcaggtg ggtatagcgc tgcaccgaaa aggccaccgg gtccagggaa atggtggtgc
    24121 gcttgccgtt ggtgtagcgg ccttcggcca gcagctccat gctgccctgg aaagtggcgg
    24181 tcttgttctt gtcctgcatg accaggattt gatagttcag cgagccattg tcgaccgaaa
    24241 agctggcggc gcgaataccg ggcgac
//
LOCUS       DASTNK010000085         7926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_801169, whole genome shotgun sequence.
ACCESSION   DASTNK010000085 DASTNK010000000
VERSION     DASTNK010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801169"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..737)
                     /gene="recX"
                     /locus_tag="VFR20_06680"
     CDS             complement(120..737)
                     /gene="recX"
                     /locus_tag="VFR20_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEU0230237.1"
                     /translation="MPRIPDDESSSEPFEQLAPSPRQTVDGGGDGAKPGIGGEGPAQQ
                     AGNTRQVGKSPLGGRSLKARAVAYLSRREYSRLELRRKLSPHAQDPTELERVLDDLER
                     EQWLSDDRFARALVHRKAARQGTARIVQELRRHGLPDERLDAVRTQLQGTELERAREV
                     WRKKFNGPPGDAREYARQYRFLASRGFSADCLRRILGDQDDAPEA"
     gene            complement(843..1916)
                     /gene="recA"
                     /locus_tag="VFR20_06685"
     CDS             complement(843..1916)
                     /gene="recA"
                     /locus_tag="VFR20_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU0230238.1"
                     /translation="MDDKTGKVAAAERGKALAAALSQIEKQFGKGSVMRYGDNQVEHD
                     IQVVSTGSLGLDIALGVGGLPRGRVVEIYGPESSGKTTLALQVIAEMQKIGGTCAIVD
                     AEHALDVQYAAKLGVNLSDLLISQPDTGEQGLEITDALVRSGSVDLIVVDSVAALVPK
                     AEIEGDMGDSLPGLQARLMSQALRKLTANIKRANCLVIFINQIRMKIGVMFGNPETTT
                     GGNALKFYASVRLDIRRIGSIKRGDEVVGNETRVKVVKNKVAPPFKQTEFDIMYGSGI
                     SREGEIIDLGVQAGIVDKAGAWFSYDGNRIGQGKDNVREYLKEHADMAFEIENKVRAQ
                     FDVAARAAGVRVPQAEVEAATGE"
     gene            2738..3637
                     /locus_tag="VFR20_06690"
     CDS             2738..3637
                     /locus_tag="VFR20_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEU0230239.1"
                     /translation="MWFKNLKVFSLSPSWAISADALQAALEKQAFQGGDSQAMQSLGW
                     ISPSEDDRLVHELNGQYLLTLRAEKKLLPTTVVNQVARARALEIEEQQGYKPGRRQMK
                     EIKEQVTDELRPRAFSVYRDTRVWIDTTNRWLIVDAASSTKSDEVLGVLAKTLDPFPA
                     TPLHVEHSPASMMTRWLVDDEPPAGFSIDQDTELRSTGEGRAAVRYVHQTVELDDVRR
                     HVQAGKQCTRLAMTWADRVSFVLSDALDVKRVTPLDVLTENGNTTTGDPREQFDADMA
                     LMTGELSRLLADLVEALGGRKAA"
     gene            complement(3696..4988)
                     /locus_tag="VFR20_06695"
     CDS             complement(3696..4988)
                     /locus_tag="VFR20_06695"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEU0230240.1"
                     /translation="MSVSQQVFLRDAMRRLNLTRDVFSARIGVKRRALDTWLLPEGSQ
                     EFRAMPDVVTRFVTEIIDNDLLLQKYAQSAQSGPLRDRIATEGKHQLLSVDQFTRESV
                     EELFRIADLMQPIARRRKVSRVLEGAVLGNLFFEASTRTRVSFGAAFCRLGGSVCDTT
                     GFTFSSMAKGESIYDTSRVMSGYVDAMVIRHPERGSVAEFARATNIPVVNGGDGPGEH
                     PSQALLDLYTILTEFSRLGKLLDGAHIAMVGDLKYGRTVHSLIKLLALYRGLKFTLIA
                     PRGLEMPEYLLEQAGKHGHVIVQTGSLEEGLAGADVIYATRVQKERFASEELEGYTPD
                     FQVNKAIIDKCCGPDVIIMHPLPRDSREGANDLSVDLNHDPRLAIFRQTDNGIPVRMA
                     IFAVLMGVEGLVQHSLRDVTWAPPSHIGPDDSVFHGVD"
     gene            5241..5591
                     /locus_tag="VFR20_06700"
     CDS             5241..5591
                     /locus_tag="VFR20_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0230241.1"
                     /translation="MDDSSMYPAASRELGAKRRELAPKQAEAFKAFGEAVFADGALPQ
                     KTKELIAVAVAHATQCPYCIRGHTERALRKGATEQELMEAIWVAVEMRAGGAYAHSNL
                     ALDTMNHAAGKHSA"
     gene            complement(5690..6250)
                     /locus_tag="VFR20_06705"
     CDS             complement(5690..6250)
                     /locus_tag="VFR20_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230242.1"
                     /translation="MADNQNSERTGQAHAYHALATVFAQGFRFLAWINGLGVLFSLCA
                     AVGIVSTNVAPDLFRIPLTTYIAGLALCGVGLLWSYLVQTLLFSQLVEGRHRRTHWVP
                     LACTLLAYGLSLVMFVAGCWVMLHLAELANQNPAYSSQSYGGQGGDEATPLNQSGSAS
                     QIVEASPVEIVLRVPSRSDTTLPGVS"
     gene            complement(6360..6923)
                     /locus_tag="VFR20_06710"
     CDS             complement(6360..6923)
                     /locus_tag="VFR20_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230243.1"
                     /translation="MSLFRALGRGAVVASLLLLAACQSMPAKKSASANSPAQATATTQ
                     PETSGATKPETTLHETNHGALVAVYLADTQQQAGWTEVKLKSGVLYVNPMPVITRADL
                     SNIRAGASKDGVGLLALGLNEAGQSKILDVTTQNPNRRLALVVGHTMLSAPSYTVPIT
                     SDQLVFPVGTEQNATAAARAIAGEPAN"
     gene            complement(7206..>7926)
                     /locus_tag="VFR20_06715"
     CDS             complement(7206..>7926)
                     /locus_tag="VFR20_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEU0230244.1"
                     /translation="ARARTFGLDSPLTTSFWSAVKTGTSKDMRDNWCVGWSRFYTVGV
                     WVGNSSGDSMRNVSGVSGAGPIWHDVMAHLSRNELSQEPPMPASVTRQRVVFDDDIEP
                     ARSEVFLGDTAMRVVRLSRELVQAGHGMPRIQQPVSGTILALDPDIPPANQRTWFRAA
                     NLAAEEARKVQWSVDGKIIGQGGEVPWMPWPGRHVVRLLSLDGKTLDRAELEVRGAVP
                     RQSAGAISAAYQARGARPARR"
BASE COUNT         1529 a         2563 c         2376 g         1458 t
ORIGIN      
        1 ccagccccgc catcatcgtg ctgggtgagg tggtccgata cgcccaggtg aacaacaagc
       61 ccgagtcact catcgcgggt cggctcaaac agacatcatc gatgggcact tgcggatagt
      121 caggcttcgg gggcgtcatc ctggtcaccc aggatgcgtc gcaggcaatc ggcggaaaaa
      181 ccgcgcgacg ccaggaaacg atattggcgg gcgtactccc gggcgtcgcc gggcggaccg
      241 ttgaatttct tgcgccagac ttcacgggcg cgttcgagtt cggtgccctg caattgcgtg
      301 cgcacggcat cgaggcgctc gtctggcagg ccatggcggc gcaattcctg cacgatgcgc
      361 gcggttcctt gccgagctgc cttccgatgc accaaggctc gggcgaaacg atcgtcggac
      421 agccattgtt cgcgctcgag gtcatcgagc acgcgttcga gctcggttgg atcctgtgcg
      481 tgagggctca gcttgcgccg cagttcaagt cgtgaatatt cgcgccggga tagataggcg
      541 acggcgcgcg ccttgagact gcgcccgccc aatggggatt ttccaacttg ccgagtattc
      601 cccgcttgct gagcgggacc ttcaccaccg atgcccggct tcgcgccatc gccaccgccg
      661 tcgaccgtct gccgtggcga tggcgcgagt tgctcgaaag gctcggaaga tgactcgtcg
      721 tcaggaatgc gcggcatgga cctcgatggt gtcttgtgtc ctgaattgga gattcgggag
      781 tccagccggg tcatgggcaa agcacagggg ctttgtgtgg atgaacccag ctggttgggc
      841 ccctattcgc cggttgcggc ttcgacctca gcctgaggca cccgcacccc ggcggcgcgt
      901 gccgcgacat caaactgcgc gcgcaccttg ttttcgattt caaaggccat gtcggcatgt
      961 tccttgaggt attcgcggac gttgtccttc ccctgaccga tacggttgcc gtcgtagctg
     1021 aaccatgcgc ccgccttgtc gacgataccg gcctgaacac cgaggtcgat gatttcaccc
     1081 tcgcgcgaga taccgctgcc gtacatgatg tcgaattcgg tttgtttgaa tgggggcgcc
     1141 accttgttct tgacgacctt gacacgggtt tcgttgccga cgacctcgtc gccacgcttg
     1201 atggagccta tacggcggat atcgaggcgc accgacgcat agaacttgag cgcgttgccg
     1261 ccggtggtgg tttcgggatt gccgaacatg acgccgatct tcatgcggat ctggttgatg
     1321 aagatgacca ggcaattggc ccgcttgatg ttggcggtca gtttgcgcag cgcctggctc
     1381 atgagccggg cctgcaggcc gggaagcgaa tcgcccatgt cgccttcgat ttcagccttg
     1441 ggcaccagtg ccgcgacgga gtcgacgacg atgaggtcga ccgagcccga acgaaccagg
     1501 gcgtcggtga tttccaggcc ttgttcgccg gtgtcgggtt gcgatatgag caaatcggac
     1561 agattcaccc cgagcttggc ggcgtactgg acgtcgagtg cgtgttcggc gtcgacgatg
     1621 gcgcacgtgc caccgatttt ctgcatttcg gcgatgacct gcagagccag cgtggttttc
     1681 ccggaagatt ccggtccgta gatttcgacc acccggccgc gcggcagccc accgacgccc
     1741 agggcgatgt ccagccccag cgaccccgtg gacacaacct ggatgtcatg ttcgacctgg
     1801 ttgtcgccat aacgcatgac cgagcccttg ccgaattgct tttcgatctg ggataaggcg
     1861 gcggcaagcg ccttgccgcg ttcggcggcg gcaaccttgc cagttttgtc gtccatgtgg
     1921 gatccttggt aaaaacggaa aatcggatgg gcctggcccg gcagttaagc ttgactgtta
     1981 cggctgaccg ttctgtccgg ctgttacgac cggctgttac caccggccac tacgcccggg
     2041 ctcgaagcca aagccagatt attgcaccaa attatactgt atcaataaac agtaatccat
     2101 actccttcac gggatgcgcc cggggtgcct cgcagaagcg cgccgccaag ccaccagata
     2161 cgccatcgtc catgccagga cagtcacagg cgcggctaca acggctacaa cggctacaac
     2221 ggctacaacg gctacaacca aggcgtcaac agcatgacag aaaccccggc caggaacaat
     2281 caagcgcgcc gcaaacttgt cccactgcag tttccacgcc cctcggccgc agcgtctgtg
     2341 attccggctt ttcgcgcgcg gttgcgcgca tccgtgccag ccggctcgaa caccccgaga
     2401 atcacaaacc ccggatctca cacccatgtc gcgggttcag gctttagtca ggcctgatct
     2461 ctagaatgac cagcgcaggc acggttcgtg cattcccatt agaaacgact gatccccctg
     2521 ttttctaatg gcatgggcca ctgcccgccg cccgcaacgt gctccccgcg agttttacat
     2581 gcacggtgcg cgcgcttatc agcggccccg tgcctaccgg cacgccgggc cctttttttt
     2641 ggcccgcgcc gacgggctta caatccccac tcgccatttt ccaaatcgcg cattccaccg
     2701 cacggcatgc gcgcctcatc ctacaagact cgtctccatg tggttcaaga atctgaaagt
     2761 attttccctt tcgccttcct gggcgatctc cgccgacgcg ctgcaggcgg cgctcgaaaa
     2821 acaggccttc caaggtggcg actcccaagc catgcagagc ctgggctgga tctcccccag
     2881 cgaggatgat cggctcgtcc acgaactcaa cggccaatac ctgctgacgc tgcgcgcgga
     2941 aaaaaagctc ctgccgacca cggtggtcaa tcaggtcgcc cgggcgcgcg cgctggaaat
     3001 cgaagagcag caaggctaca agcccggccg caggcaaatg aaggaaatca aggaacaggt
     3061 cacggacgaa ctgcggcccc gcgccttttc ggtgtatcgc gacacgcgtg tatggatcga
     3121 cacgaccaac cgctggctga tcgtcgatgc cgcctcgtcc accaaaagcg acgaggtcct
     3181 gggcgtgctg gcgaaaacgc tggacccgtt tccggcaacc ccgctgcacg tcgagcattc
     3241 acccgcgtcc atgatgacgc gctggctggt ggatgacgag ccacccgccg gcttcagcat
     3301 cgaccaggac acggaactgc gctcgacggg cgaaggccgc gccgcggtcc gctacgtgca
     3361 ccagaccgtc gagctcgacg acgtgcggcg ccatgtccag gcgggaaaac aatgcacccg
     3421 gcttgccatg acctgggccg accgggtgtc gttcgtgctc tccgacgcgc tggacgtgaa
     3481 gcgcgtgaca cccctggacg tcctgacgga aaacggcaac acgaccaccg gcgacccgag
     3541 ggagcaattc gacgcggaca tggcgctgat gacaggcgag ctctcgcggt tactggccga
     3601 tctggtcgaa gccctgggcg gtcgtaaagc cgcatagcga ggcaagctcc aggcgcccgg
     3661 gacggcgcgg gcgcctttca cagggtggat aacccctaat ccacgccgtg gaacacactg
     3721 tcgtcggggc cgatgtgcga cggcggcgcc caggtgacgt cccgcagcga atgctgcacc
     3781 agcccctcca cgcccatcag caccgcgaat atcgccatgc gcacggggat gccgttgtcg
     3841 gtctgccgga atatcgccag gcgcgggtca tggttcagat ccacgctgag atcgttcgcc
     3901 ccttcccggc tgtcgcgcgg cagcgggtgc atgatgatga cgtcggggcc gcagcatttg
     3961 tcgatgatgg ctttgttcac ctggaaatcg ggcgtgtagc cttcgagttc ctcgctcgcg
     4021 aagcgctctt tctgcacgcg cgtggcgtag atcacgtcgg cgccggccag cccctcttcc
     4081 agcgacccgg tttgcacgat cacgtggccg tgcttgccgg cctgctccag aaggtattcc
     4141 ggcatctcga gcccgcgtgg cgcgatcagc gtgaacttca gtccacggta cagcgccagc
     4201 agcttgatca gcgaatggac cgtgcgcccg tatttcagat cacccaccat ggcgatgtgc
     4261 gcgccgtcca gcagtttgcc caggcgcgag aactcggtaa ggatggtgta gagatccagc
     4321 agcgcctggc tggggtgttc gccgggcccg tcgccaccgt tgaccacggg aatgttggtg
     4381 gcacgcgcga attcggccac cgagccgcgt tccggatggc gaatcaccat ggcatccacg
     4441 tagccgctca tgacgcgact ggtgtcgtag atcgattccc ctttggccat cgacgaaaag
     4501 gtaaagccag tggtgtcgca caccgagccg cccaaccggc aaaacgccgc gccaaagctg
     4561 acccgggtgc gggtgctggc ttcgaagaac aggttgccca gcaccgcgcc ctccagcacg
     4621 cgcgacacct tgcggcgtct ggcgatgggc tgcatcaagt cagcgatgcg gaacagctct
     4681 tccacggatt cgcgggtgaa ctggtccacg gaaagcagct gatgcttgcc ctccgtggcg
     4741 atgcggtcgc gcaacggccc ggattgcgcg ctttgcgcat acttctgcag cagcaggtcg
     4801 ttatcgatga tctcggtgac gaaacgcgtc accacgtccg gcatggcgcg gaattcctgg
     4861 gagccctccg gcagcagcca ggtatcgaga gcccggcgtt tgacaccaat gcgcgcggaa
     4921 aaaacgtcgc gcgtcagatt cagacgccgc atggcgtcac gcaaaaaaac ctgttgggac
     4981 acggacatat ggatttcccc ttgaagatga agaattaatg tgcgcaatgc gtatatttta
     5041 agcccgcatg acctccatcc tgaataaaac aacaaaatac tttgtgttcc gttgtattca
     5101 tacccctgtc atctgtacgc aggcgagaca ctccctcaat accccgacct ataatgaatt
     5161 cgcacctcag gcccaccggg ccgcagacgc gtccggcgca caggccagcc taacccgtac
     5221 tcacgacaag gagcttttga atggatgatt caagcatgta tcccgccgcc tcgcgggaac
     5281 tcggcgccaa gcggcgcgaa ttggcgccca aacaagcgga agccttcaag gcgttcggcg
     5341 aggcggtctt cgccgacggc gccctgcccc agaaaaccaa ggaactcatc gccgtggcgg
     5401 tggcccacgc cacgcaatgc ccttactgca ttcgcggcca cacagaacgg gcgctgcgca
     5461 agggcgccac ggaacaggag ttgatggaag cgatctgggt tgccgtggaa atgcgggccg
     5521 gcggcgctta tgcgcactcg aatctggcgc tggacaccat gaaccacgct gcgggaaagc
     5581 actcggcctg agcctgtgcg ctcgagtcgg tgcgcgcgca acgcctccgg cgccgttagc
     5641 aaggcggggg caaccatgca gcaggccccg cccccggaga aacgccgcct tatgaaaccc
     5701 cgggcagcgt cgtgtcgctg cgcgacggga cccgcagcac gatttccacc ggagacgcct
     5761 caacaatctg cgacgcgctc ccggactgat tgagcggcgt agcctcgtcg ccaccctggc
     5821 caccgtacga ctgcgaggaa taagccgggt tttgattggc cagctcagcc aggtgcagca
     5881 tgacccagca tccggctacg aacatcacca ggctaaggcc ataggccaac aacgtgcatg
     5941 ccagcggcac ccaatgggtg cgccggtgcc ggccttcaac caactggctg aacaacaacg
     6001 tctggaccag gtacgaccaa agcagcccga ccccgcacag ggccaggccg gcgatatagg
     6061 tcgtcaacgg gatgcggaac agatcgggcg ccacgttcgt gctgacaata ccgacggccg
     6121 cgcacaacga aaacagcaca cccagcccgt tgatccaggc caggaagcga aacccttgcg
     6181 caaacaccgt cgccaacgcg tgatacgcgt gagcctgacc ggtgcgttca ctgttctgat
     6241 tgtctgccac ggcgtggctt ccccggggaa ggcgccgagc cacgagactc ggcgccagta
     6301 ttgtggcccg ctatgtagac ttgtgcgcgg gtgccattgt ttcagtcgcc ggacgaacct
     6361 taattcgccg gttcacccgc gatggcgcga gccgccgctg tggcgttttg ctcggtgccg
     6421 acagggaaca cgagttggtc ggaagttatg ggtaccgtgt agctgggtgc cgacaacatg
     6481 gtgtgcccca cgaccagcgc cagccgccta ttgggattct gcgtggtgac gtccaggatc
     6541 ttcgattggc cggcctcgtt caggcccagc gccaagaggc ccacgccatc cttgcttgcg
     6601 ccagcgcgga tattgctgag gtcggcccgg gtgatgaccg gcatggggtt gacatacaac
     6661 acccccgact tgagtttgac ctcggtccaa cccgcctgct gctgcgtatc ggcaagatac
     6721 acagccacca gcgcgccatg attggtctcg tgcaaggtgg tttcaggctt ggtggcgccg
     6781 gaggtttccg gctgggtggt cgccgttgcc tgagccggcg aattcgccga cgcgctcttc
     6841 ttggccggca tggactggca ggcagccagc aaaagaaggc tggcgacgac agcgccacgg
     6901 cccagcgcgc ggaatagact catgatgcac ccctcatctg acgaagcagt aacaattcta
     6961 acagggcgcc ttgatgcccc agcacaatat ttgtcaactt ccgagaacaa atatgaaaat
     7021 tcgcaacgcc ccgatacgga gaatgccgaa tggataaaat tcaatgcaac gtaagccggc
     7081 gcatgccata ctatcggctt gttcatttca tccttctgcc gtggagtccc ctgtggctac
     7141 taacgaagac aaacccaaat tctcggcggg gctggtcacg accgtcgtca cgctcgtgtt
     7201 cttcatcatc tacgcgctgg tcgtgctcca cgggcttgat acgccgcgct gatcgcgccg
     7261 gctgattgcc tcggcaccgc gccgcgcacc tccagctcgg cgcgatccag tgtttttcca
     7321 tccaggctca gcaagcggac aacgtgcctg cccggccatg gcatccaggg cacctcgcca
     7381 ccctggccga tgatcttgcc atccacgctc cattgcactt tccgcgcttc ttcggcggcc
     7441 aggttcgctg ctcggaacca tgtgcgctga ttcgcgggcg gaatatccgg atccaatgcc
     7501 aggatcgtcc cgctgacagg ttgttgaata cgtggcatgc cgtggcccgc ctggaccagt
     7561 tcgcggctca ggcgcaccac ccgcatggcc gtatcaccaa gaaaaacctc gctgcgcgcc
     7621 ggctcgatgt catcgtcgaa cacaacacgc tggcgcgtga cactcgcggg catgggcggc
     7681 tcttggctga gctcattgcg cgacaaatgc gccatcacat catgccagat gggtcccgcc
     7741 cccgaaacgc ccgacacgtt gcgcatgctg tcaccactgc tgttcccgac ccacaccccc
     7801 acggtgtaga agcgcgacca ccccacacac cagttgtccc gcatgtcttt gctggtaccc
     7861 gtcttgacgg cggaccagaa cgacgtggtg agcgggctat ccaggccgaa ggtgcgtgcg
     7921 cgcgcg
//
LOCUS       DASTNK010000086        14483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_60805, whole genome shotgun sequence.
ACCESSION   DASTNK010000086 DASTNK010000000
VERSION     DASTNK010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14483
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60805"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(641..1783)
                     /locus_tag="VFR20_06720"
     CDS             complement(641..1783)
                     /locus_tag="VFR20_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEU0230245.1"
                     /translation="MAELNQTAGISVPPVARAGDPLDAIDTPSLILDLDVFEDNLRTM
                     QVLAERHGVALRPHAKAHKCPDVALRQIALGAVGICCQKVSEAVPFVQAGVRDVHISN
                     EVLGTPKLALLARLAGQASMTVCVDHARALEALGAAVQAQGVRLGVLVEIDVGQKRCG
                     VQNPADAVALARRVLGTPGLEFMGLQAYHGGLQHKRSLMLRQRACHRAARRVKKFLAA
                     FARAGIECRRVTGGGTGSAEFDVACGVYTEIQAGSYPFMDAQYASIDWDDSLSFKHSL
                     FLMGTVMSAPRPDRVVVDVGLKSTSAECGVPRALPGAHWRCVAINDEHTILHASRAGV
                     LPALGARVRLIPSHVDPTFNLHDAVVAVRGQKVEAVWPISARGLSR"
     gene            2060..2521
                     /locus_tag="VFR20_06725"
     CDS             2060..2521
                     /locus_tag="VFR20_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HEU0230246.1"
                     /translation="MFDILVYLFENYYTPQACPEADVLAHKLAAAGFEHEDINEALGW
                     LNGLAQTTEQCLDLAQIPQTSSVRVYTNDEYQTLGTQAIGFITFLENSDVLPPVLREI
                     LIDRALATLESPITLEKIKIMALMVLWSQEAEIDHLVFEELLADDQSRLSH"
     gene            2549..3484
                     /gene="gluQRS"
                     /locus_tag="VFR20_06730"
     CDS             2549..3484
                     /gene="gluQRS"
                     /locus_tag="VFR20_06730"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEU0230247.1"
                     /translation="MSFTPPAGRGYVGRFAPSPSGPLHEGSLVAAMAGFLDARVHAGR
                     WLLRIEDIDEPRTVPGADRLIMRQLRALGMEWDGGPVWQSRRHALYQRSFDALKAAGG
                     VYGCACTRRDIELALAAQGPGTAKQAAGGLRERPYPGTCRNGLAPGRRTRAWRFRVPD
                     GVERFDDRWQGRQSQDVARDVGDFVLKRADGLWAYQFVVVVDDSHQGVTDIVRGADLL
                     DSTARQRVLARALGLECPRTLHVPLVLDDRGLKLSKQNHAPALDERKPVDTLNAAWRR
                     LGFETLPVSGVPEFWIRARTIWGRRFGAASPARHT"
     gene            3705..6437
                     /locus_tag="VFR20_06735"
     CDS             3705..6437
                     /locus_tag="VFR20_06735"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEU0230248.1"
                     /translation="MNKTLIIAEKPSVALDISRALGGFTREGDYFESENYVLASSIGH
                     LLTLVAPNDPVRGKWSFAHLPVIPPQFELKPADKRSTERLKLLVKLLKRKDVDALINA
                     CDAGREGELIFRYIVQYAGVKKPVSRLWLRSMTQNAIREAFANLREDDVMKPLEAAAR
                     SRAEADWLVGINGTRAMTAFNSKDGGFFKTPVGRVQTPTLAIVAEREERIRQFVPRDY
                     WEVHATFVAAAGFYTGRWFDPKFVKDERDPEKRDSRLWSQNAAQTIVSACRDRPGTVT
                     EESKPSTQLSPALFDLTSLQREANSRFGFSAKTTLSLAQTLYERHKALTYPRTDSRYL
                     PEDYLATVKKTAQALAEGGSAAAASVQPFARQLCGESWIKPNRRIFDDKKVSDHFAII
                     PTLQVPRDLSDAEHKIYELVARRFLAVFFPAAEFRVTTRVTEVSGHHFKTEGKVLVAP
                     GWLAIYGRESQGEGDNATLTPVANNETVKTDEIEARGLATRPPARFNEATLLSAMEGA
                     GKLVDDEALREAMSERGLGTPATRAAIIEGLLNEGYLRREGRELVPSAKTRQLMTLLS
                     GLRVSELTSPELTGEWEHRLKQIEQRQLDREAFMREIAQMTQVIVKRAKEYERDTVPG
                     DYATLSTPCPKCGGVVKENYRRYACTQCDFSIGKHPGGRTFEIAEVEQLLKDRTLGPL
                     PGFISKMGRPFAALLRITDEYKLEFDFGQREGENDEPIDFSDQTSLGACPKCGHAVFD
                     HGMSYVCEKSVGPDKTCDFRTGKMILQQEITPVQVAKLLANGKTDLLDGFVSSRTNRK
                     FKAFLVTQAGGKVGFEFEPRPVKPGRATKTVAAATADTAEAPAAKKGAAKKATKTATK
                     TAAKKAAAPGTAKPVAKAATKTARKSPAKTAAKKTARKASSAQTKTLDDDDPPF"
     gene            6652..7527
                     /locus_tag="VFR20_06740"
     CDS             6652..7527
                     /locus_tag="VFR20_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0230249.1"
                     /translation="MKKRTWQVIGALCVALGYSAAAAAAQEVDAIVKKLEATHTITMG
                     YRETIKPTSFLNDQHQPDGYAVDMCKQIIADTQKELKLPELKIKYVPINIQNRQALVA
                     NGTVDIACGGAVHTFARARQVDFSPVSYVSSNQLLVLKDSGIKGFQDLNGKVVAVPTA
                     GNTEPILNEIIKKNKLNIKVLHVDTHAAALIDIESHRADAYFCDNSAFLHMIKEARNP
                     SSLAVVGPEYGYAPQAFMIPKNNPRFMWIVRHSMAQMYESGKAEQLFNKWFGPYGVKV
                     GPRLKAAWDTYSYPE"
     gene            7543..8259
                     /locus_tag="VFR20_06745"
     CDS             7543..8259
                     /locus_tag="VFR20_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU0230250.1"
                     /translation="MDYRWDWGVLLQQPYQDWLWTGIGWTVLISLCSWALALIIGVIF
                     GAARSLPNKPVQFVASIYVEIFRNIPALVQLFLWYFVFPNVVPNDFGMWLKRGMPDPE
                     FVTAVVAIGLFAGARVAEQVRAGIGAVGLHLLPAALATGLRPIQAFRLILFPLGLRAI
                     IAPLTSEFLITMKMSSIALTIGVLELTGQSRQIENYTFHGLEAFAFATVMYLILGLCI
                     TALMRFLNDRYGQIRQEGEP"
     gene            8256..8912
                     /locus_tag="VFR20_06750"
     CDS             8256..8912
                     /locus_tag="VFR20_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU0230251.1"
                     /translation="MSGYDWGEITRNWQYLGQGMLLSLLLVTVATVGGVLLGFGLALM
                     RISRRRAVASVATAYVTVMRCVPLILVLFWFYFLVPLVIGRPIGSLVSALIAFVMFEA
                     AFYCEIVRAGILSIRAGQTEAGLATGMRRWQTLRLIVIPQAVRAMVPLLLNQIIIVFQ
                     DTSLVYVVALTDFMTAASVLAQRDGRPTEVYTFVAIVYLIICFTLSKSVAYYKARGSK
                     "
     gene            8909..9637
                     /locus_tag="VFR20_06755"
     CDS             8909..9637
                     /locus_tag="VFR20_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU0230252.1"
                     /translation="MIVLDSVHKCYGRTPVLNNCSATVKLGEVVVICGPSGSGKSTLI
                     KCVNGLVPFDRGTITVDGISVGDKRTDLPALRTRIGMVFQNFELYPHMTALQNVSLAQ
                     IHVLKRSRAEADARSEQLLARVGLANKLSSRPGQLSGGQQQRVSIARALAMDPKAMLF
                     DEPTSALDPEMVNEVLEVMQALATEGMTMMVVTHEMGFAKRVAERVLFMDAGEILEDS
                     PKEQFFAAPSTERARQFLTKVLSH"
     gene            9732..10811
                     /locus_tag="VFR20_06760"
     CDS             9732..10811
                     /locus_tag="VFR20_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HEU0230253.1"
                     /translation="MRVSILGAGAIAFGTAAYLARAGHDPMLWSPSGRRTRALAGGAR
                     LVARNAVEGEFSPRVAASCAEAVAQAEVVMLALPGYGHKAVLDAAAPHLRVGQPVIIS
                     SHLSFGALYLSRLLAARGVKAPIVAWGTTLTTGGQPSLTEAFVSTVRQKVDMATLPHS
                     YADEGLALCTALFGDHFVAREGLLAIALSNLNPQNHLGIAMLNLTRMERGEEWNQSQN
                     VTPAVGRLIESLDAERLAIAEAFSLSVKTVRQHFSLSFHVPEDSVSAMNQDMFRRGHS
                     GLGPKTADSRYVLEDVPFGLVPTARLGRLAGRPAPLHEAGIALFSAAYGRDFPAENNL
                     LPGLDLPERISIEALIDLTRGGYSK"
     gene            complement(10886..11824)
                     /locus_tag="VFR20_06765"
     CDS             complement(10886..11824)
                     /locus_tag="VFR20_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0230254.1"
                     /translation="MRYDLIDLRLFVAIADAGNISRGGAACFLAPSSASLRIKQLEET
                     LGAQLFKREARGVSLTSAGQVMLQHCRACLAELEQMHADLAPYAKGIKAHVALFASST
                     AVESFLPHDLQSFLRDYPEVRIVMSERLSHETLAAVAAGQADVGIVTWDGHHPELDFM
                     PYRENELMAVLPETDSLAAREEVSFADCLERPFVCAQGGSAIYTFIVNMATSLGRQLD
                     IRIQVASFASMLSMIRAGVGVGVMPRPVLQLLNCEGVRILPISDEWATRPLRICTRRN
                     STLSPYVRALLDCLDTARQTPFNPMQTVAAPGLVRG"
     gene            12038..13483
                     /locus_tag="VFR20_06770"
     CDS             12038..13483
                     /locus_tag="VFR20_06770"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0230255.1"
                     /translation="MTPTTPTTATLPIGLSALERRLAQDLAWLDLPARQWVAPGSVNG
                     QALLDVAIIGGGMAGLAAATAMTHMGIRARIYDKAPQGFEGPWATTARMETLRSPKQL
                     TGPALGLPSLTFRAWFEAQYGIAAWDALDKIPRLQWMDYLRWYRRVMNLDVHNEHHVD
                     SIVPQADHVRLRLQTPSGPQRVLARHVVLATGRAGLGGPFIPGFVENLDRRLWAHSSD
                     EMDYAALRGKRIGVVGAGASAMDSAATALENGAAHVDLIIRRPDLPRVNKGKGAGSAG
                     FTNGYVHLPDDWKWRIRHYINVQQVPPPRASVLRVSRHDNASFNLGSPILDVAPRGGR
                     LVLRTPRKTFDLDFLIIATGFRVDWQARPEYAGIAPHVRLWGDRYQPEPGQEDKELHD
                     SPDLGPVFEFQEKPENPLPGLSRVHCLCYPASLTHGTVSGDIPAVSDGALRLSRGMAS
                     LLFNENIDHHYRDLLAYDEPEVFGDEWTPAA"
     gene            13681..>14483
                     /locus_tag="VFR20_06775"
     CDS             13681..>14483
                     /locus_tag="VFR20_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU0230256.1"
                     /translation="MTKRASAHPTRSVIASGSPGTVPIAPALDTTMEDARITRGSTTL
                     GRRVLWISLLAIILGAIAALIAQLLMFMINIITDVTFYGRISDVVGPAAAGRTGSFVL
                     SPAHNQLGLWVILMPAIGGLIAGIMARWGSRAIQGHGIPEAMEQILQNESNIPPRVTW
                     LKPVSSAFAIGTGGPFGAEGPIISTGGALGSLIGQLLSVTATERKTLLAAGAAAGMAA
                     VFGAPVSSLVMAIELLLFELRPRSLIPVALAAVTATGVRYATYGPGAVFA"
BASE COUNT         2622 a         4903 c         4527 g         2431 t
ORIGIN      
        1 ggccggcgcg tccagcggtc gtggcgctgc gcgccccgac tgcccgggtc tgcatcgtcc
       61 gggcgggcgt cgggcgaaac tcgttgtgcc tcgcgtactc gcgaggcaca actcagacag
      121 cgcccgacga ctgcccccgc cctcccttcg tcagcacccg gcgctggccg gaacgccggc
      181 ccgcactgct tcacccaccc cgtggctacg acaatggcat gcatggaggt atgcgccgga
      241 accgcgggcg accgggcaca tcaaacacga gcggcaaccc aatttatgtc tgccgcgcgt
      301 aacagcggat gggtccactc ccccgcatgc catcaccgcg agctgtctgt cggggcttgg
      361 ggcgaggccg gtgtaaggcg tgcgccggat tcgtcgtggg agggcggggg ccgtcgtcgg
      421 gcgctgtctg agtgttggga tcagcccccg gctgatccca acgcgagttt cgcccgacgc
      481 ccgtccggac acgacggatc cggatgaagc acgccgtgcc ggtcacgccc caagccccga
      541 cagacagcgt cttcagatac cagacaggat cttcaaacaa cccacaacac ccttgaattc
      601 caaacagcgc cgagaacgaa gcaaacaatc aaaccacgcg ctaacgactc aacccacgag
      661 cgctgatcgg ccagacagcc tccaccttct ggccacgcac cgccaccacc gcgtcatgca
      721 ggttgaacgt gggatccaca tggctgggaa tcaagcgcac ccgcgccccc agggccggca
      781 acacacccgc gcgcgacgca tgcaggatgg tgtgttcatc attgatggcc acgcagcgcc
      841 agtgcgcgcc gggcaacgcg cgcggcacgc cgcattcggc gctggtggac ttcagcccca
      901 catccaccac cacccgatcg ggcctgggcg cgctcatcac cgtgcccatc aggaaaagac
      961 tgtgcttgaa cgacagcgaa tcatcccaat cgatggacgc gtactgcgca tccatgaacg
     1021 ggtacgaccc tgcctggatt tccgtgtaaa ccccgcaggc cacgtcgaat tcggccgacc
     1081 cggtgccgcc gccggtgacg cggcggcatt cgatgcccgc gcgggcgaat gccgccagga
     1141 acttcttgac gcgccgcgcc gcccggtggc aggcgcgttg gcgcagcatc agcgagcgct
     1201 tgtgctgcag gccgccgtga tacgcctgca gccccataaa ttccagccct ggcgtgccca
     1261 gcacgcgccg ggccagcgcc accgcgtcgg ccgggttctg cacgccgcac cgcttctggc
     1321 cgacgtcgat ctcgaccagc acgccaagcc gcaccccttg cgcctgcacg gcggccccca
     1381 gcgcctcgag ggcacgcgcg tgatcgacgc agaccgtcat cgaggcctgg cccgccaacc
     1441 gcgcgagcag cgccagcttg ggtgtgccca ggacctcgtt gctgatgtgc acgtcacgca
     1501 cgcccgcctg cacgaacggc acggcctccg agactttctg gcaacagatg cccacggcgc
     1561 ccagggcgat ctggcgcaac gcgacatcgg ggcatttgtg ggccttggcg tgcgggcgca
     1621 gcgccacgcc atggcgttcg gccagcacct gcatggtgcg caggttgtcc tcgaacacgt
     1681 cgaggtcgag aatcaggctg ggcgtgtcga tggcatcgag cggatcgccg gcccgggcca
     1741 cgggcggaac actgatgcca gccgtttggt tgagctccgc catgggttcc tcccgcctta
     1801 tgcgcatggc gcgccacagc gggcacgcca gagggttcag ccagccttgc gacgccatat
     1861 ggtgcgccgc gaagcgttgt cgggagccag gataacgact ccagagggcg agtgtacatg
     1921 aagcggggcc gacgcggccc cgccccgccg accaccccgt ccgcacaccc aggttgcccg
     1981 cattgccaaa tcagcgtaca gtgacttccg actctatagc ggctaaccct cagactgttt
     2041 tcaagggcaa ccgaccatca tgttcgatat tctggtttat ctgttcgaaa actactacac
     2101 cccccaggca tgccccgagg ccgacgtcct ggcccacaag ctcgccgccg ccggattcga
     2161 gcacgaagac atcaacgagg cactgggctg gctcaacggg ctggcgcaaa ccaccgagca
     2221 atgtctcgat ctcgcgcaaa tcccgcaaac cagcagcgtg cgcgtctaca ccaacgacga
     2281 ataccaaacc ctgggcacgc aagccatcgg tttcatcacg ttcctggaaa actccgacgt
     2341 gctgccgccc gtgctgcgtg aaatcctcat cgaccgcgcg ctggcaacgc tcgaatcgcc
     2401 cattaccctg gaaaagatca aaatcatggc gctcatggtg ctatggagcc aggaagccga
     2461 aatcgaccac ctcgtgttcg aggaactgct ggccgacgat cagtcccggc tgtcgcactg
     2521 acgtccggtt ccgggccctg tcattcctgt gtcgttcact cccccggctg gccgcggcta
     2581 cgtcgggcgc ttcgcgccca gtccgagcgg cccgctgcac gaggggtcgc tggtcgccgc
     2641 catggcgggg tttctcgatg cgcgggtaca cgccggacgg tggctgttgc gcatcgaaga
     2701 catcgacgaa ccccgcaccg tgccgggcgc cgaccggctc atcatgcggc agttgcgggc
     2761 cctggggatg gaatgggacg gcgggccggt ctggcagtcg cggcggcacg ccctgtacca
     2821 gcgatctttc gacgcattga aggcagcggg cggggtttac ggctgcgcct gcacacgccg
     2881 cgacatcgaa ctggcgctgg ctgcgcaagg gcctggcaca gccaaacaag cggcgggcgg
     2941 gctgcgtgag cgcccctacc ccggcacctg ccgcaatggg ctggccccgg ggcgcagaac
     3001 gcgcgcctgg cgttttcgtg tgcctgacgg ggtcgaacgg ttcgacgacc gctggcaggg
     3061 gcgccaatcc caggatgtgg cccgcgacgt cggtgatttc gtcctgaagc gcgccgacgg
     3121 gctctgggcc taccagttcg tggtggtcgt cgatgacagc caccagggcg tgaccgacat
     3181 cgtgcggggg gcggatctgc tggattccac cgcccggcag cgcgtgctgg cgcgcgccct
     3241 ggggctggag tgcccccgca ccctgcacgt ccccctggtg ctggacgatc ggggactcaa
     3301 actgtccaag cagaatcacg cgccggccct ggacgagcgc aagccagtcg atacgctcaa
     3361 tgcggcctgg cggcgtctcg ggttcgaaac gctgcccgtg tcaggcgtgc cggaattctg
     3421 gatccgcgcc cggacaatct gggggaggcg cttcggcgcc gccagcccgg cccgccacac
     3481 ctgacggcgc gcaagacgaa tttgttataa atacccggcg attttcaccg gattcccatt
     3541 gactgccacc aaaatccggc aaattgactg ggcgggccgg ctccgtcgct cccgaaaagc
     3601 cagtctgata tattaattta ggtaatggcc cggacttgcg tgttgcattt cttcaaccgt
     3661 atgatccgcg ctatccgctg ccgcgacgca gccactggaa ttgcatgaac aagacactga
     3721 tcatcgccga aaagccctcg gtggccctgg atatctcgcg tgccctggga gggttcacac
     3781 gcgaggggga ctatttcgag agcgaaaact acgttctcgc gtccagcatc ggccatttgc
     3841 tgaccctggt ggcgcccaac gaccccgtgc gcggcaagtg gagcttcgcg catctgccag
     3901 tgatcccgcc gcaattcgag ctcaaacccg ccgacaaacg ctccaccgag cggctcaagc
     3961 tgctggtcaa gctgctcaag cgcaaggatg tcgacgccct catcaacgcg tgcgacgcgg
     4021 ggcgggaagg cgagctcatc ttccgctaca tcgtccagta cgcgggcgtc aagaagcctg
     4081 tgagccgcct gtggttgcgc tccatgacgc aaaacgccat acgcgaagcc ttcgccaacc
     4141 tgcgcgaaga cgatgtcatg aaaccgctgg aagcggcggc ccgctcgcgc gccgaggccg
     4201 actggctggt cggcatcaac ggcacgcgcg ccatgacggc cttcaacagc aaggacggcg
     4261 ggttcttcaa gacgccggtg gggcgggtgc agacacccac cctggccatc gtggccgagc
     4321 gcgaggaacg catccgccag ttcgtgccgc gcgactactg ggaagtccac gccacgttcg
     4381 tcgcggcggc aggcttctac accggccgct ggttcgaccc gaaattcgtc aaggacgagc
     4441 gcgatcccga aaagcgcgat tcacgcctgt ggtcgcagaa cgcggcgcag acaatcgtct
     4501 ccgcctgccg cgacaggccc ggcaccgtca ccgaggaatc caagccgtcc acccagttgt
     4561 cgccggcgct gttcgacctg acctccctgc agcgcgaggc gaactcgcgt tttggcttct
     4621 cggccaagac caccttgtcg ctcgcgcaga ccctgtacga acgccacaag gcactcacct
     4681 acccgcgtac cgattcccgc tacctgccgg aagactatct cgccactgtc aagaaaaccg
     4741 cccaggcgct ggcggaaggc ggctcggcgg cggccgccag tgtccagccg ttcgcccggc
     4801 aactgtgcgg cgagagctgg atcaagccca atcgccgcat cttcgacgac aagaaggtgt
     4861 cggaccactt cgccatcatc cccacactgc aggttccacg cgacctcagc gacgccgaac
     4921 acaaaatcta cgaactggtg gcgcgacgct ttcttgcggt gttcttcccg gcggccgagt
     4981 tccgcgtcac cacacgcgtc accgaggtct cgggccacca cttcaagacc gagggcaagg
     5041 tgctggtcgc gccgggctgg ctcgcgatct acgggcgcga atcgcaaggc gagggggaca
     5101 acgccacgct cacgccagtc gccaacaatg aaaccgtcaa gaccgacgag atcgaggcgc
     5161 gcggcctggc cacccggccg ccggcgcgct tcaacgaagc caccctgctg tcggcgatgg
     5221 aaggcgcggg caagctggtc gacgacgagg cgctgcgcga ggccatgtca gaacgtggcc
     5281 tgggcacccc cgccacccgg gcggccatca tcgaaggttt gctcaacgag ggttatctgc
     5341 gccgcgaggg ccgcgaactg gttcccagcg ccaagacgcg ccagctcatg accctgctgt
     5401 cggggctgcg cgtcagcgaa ctcacctcgc ccgaactcac cggcgaatgg gaacaccggc
     5461 tcaagcagat cgagcagcgg cagctcgacc gcgaggcctt catgcgtgaa atcgcgcaaa
     5521 tgacccaggt catcgtcaag cgcgccaaag agtacgagcg cgacaccgtg cccggcgact
     5581 acgccacgct gtccacgccc tgccccaaat gcggcggcgt ggtcaaggaa aactaccgtc
     5641 gctacgcctg cacgcagtgc gacttttcca taggcaagca tccgggcggg cgcacgttcg
     5701 aaatcgccga ggtcgagcaa ttgctcaagg atcgcacgct ggggccgctg cctggattca
     5761 tcagcaagat ggggcgcccg ttcgctgccc tgctgcgcat caccgacgaa tacaagctgg
     5821 aattcgattt cgggcagcgt gagggcgaaa atgacgagcc gatcgatttc tccgaccaga
     5881 cctccctggg ggcttgtcca aaatgcggcc atgccgtctt cgatcatggc atgagctacg
     5941 tatgcgagaa atcggtgggg ccggacaaga cctgtgattt ccgcaccggc aagatgatcc
     6001 tgcagcagga aatcaccccg gtccaggtcg ccaagctgct ggccaacggc aagaccgacc
     6061 tgctcgacgg gtttgtctcc agccgcacca accgcaagtt caaggcgttc ctggtcaccc
     6121 aggctggcgg caaggtgggg ttcgaattcg aaccccggcc ggtgaaaccg ggccgcgcca
     6181 ccaagacggt cgcggcggcc acggctgaca cggcggaggc accggccgcc aagaaaggcg
     6241 ccgccaagaa ggccaccaag accgccacca agaccgccgc gaaaaaagcg gcggctccgg
     6301 gcacggccaa gccggtcgcc aaagccgcca ccaaaaccgc tcgaaaatca ccggcaaaga
     6361 ccgccgcgaa aaagacggcg cgcaaggcgt cttccgcaca aacaaaaacg ctggacgacg
     6421 acgacccccc gttctgacga atcgacattc aacggtcgcg ccccttccct ggggcggcgc
     6481 ccacctgtgg tttttgtctg acacgaaagc aggttccggc ataagcaact tgttggagcc
     6541 ggattctgcg tgccaaactg ctttggcatc ctcccacccc tacaatggtc gcgggcgaat
     6601 gagaaaaacg cggatcccca gtgcgatccg acgacaacac aggagataag catgaagaag
     6661 cgtacttggc aggtcattgg cgcgttatgc gtcgcactcg gctattcagc ggcggccgcg
     6721 gcggctcagg aagtcgacgc catcgtcaaa aagctggaag ccacgcacac catcaccatg
     6781 ggctaccgcg agaccatcaa gcccacttcg ttcctcaacg accagcacca gcccgacggc
     6841 tacgcggtcg acatgtgcaa gcagatcatc gccgatacgc aaaaagaact gaaactgccg
     6901 gaactcaaga tcaaatacgt gccgatcaac atccagaacc gtcaggcgct ggtcgccaac
     6961 ggtacggtgg acatcgcctg tggtggcgcg gtgcatacat tcgcgcgggc ccgccaggtt
     7021 gatttctcgc cggtcagcta tgtgtcgtcc aaccagttgc tggtgctcaa ggactcgggc
     7081 atcaagggct tccaggacct gaacggcaag gtcgtcgcgg tgcccacggc cggcaacacc
     7141 gagcccatcc tcaacgagat catcaagaag aacaagctca acatcaaggt cttgcacgtt
     7201 gacacgcacg cggccgcgct catcgacatc gagagccacc gcgccgatgc ctacttctgc
     7261 gacaacagcg cctttctcca catgatcaag gaagcgcgca acccgtccag cctggccgtg
     7321 gtcggcccgg aatacggcta cgcgccgcag gcgttcatga tccccaagaa caacccgagg
     7381 ttcatgtgga tcgtccggca ctccatggcg cagatgtacg aatcgggcaa agccgagcag
     7441 ctcttcaaca agtggttcgg cccgtatggc gtgaaggtcg gcccgaggct caaggccgcc
     7501 tgggacacct attcgtatcc cgaataaggt agtcggcctg ccatggacta ccggtgggat
     7561 tggggcgtac ttcttcaaca gccctaccag gactggctct ggacagggat cggctggaca
     7621 gtgctcatca gcctgtgcag ctgggccctg gccctgatca taggcgtgat cttcggggcg
     7681 gcccgaagcc tgccgaacaa gccggtccag ttcgtggcca gcatctacgt tgaaattttc
     7741 cgcaacatcc ccgccctggt ccagttgttc ctctggtact tcgtgttccc caacgtcgtg
     7801 ccgaacgatt tcggcatgtg gctcaagcgc ggcatgcccg atccggaatt cgtcaccgcc
     7861 gtggtggcga tcgggctgtt cgccggggca cgggtggctg aacaggtgcg cgccggcatc
     7921 ggcgccgtcg gcctgcatct gctgccggcg gccctggcca cggggctgcg ccccatccag
     7981 gcgttccggc tcattctctt ccccctgggg ctgcgcgcca tcatcgcgcc gctcacatcc
     8041 gaattcctga tcacgatgaa gatgtcgtcg atcgcgctga ccatcggcgt gctcgaactc
     8101 accggccaga gccggcagat cgagaactac acgttccatg gcctggaggc tttcgccttc
     8161 gccaccgtca tgtacctgat cctgggcctg tgcatcacgg cgctgatgcg gttcctgaac
     8221 gaccgctatg gtcagatccg ccaagagggc gaaccatgag cggctacgac tggggtgaga
     8281 tcacccgcaa ctggcagtac ctgggccagg gcatgctgct gagcctgctg ctggtgacgg
     8341 tggccacggt gggcggcgtg ctgctggggt tcgggctggc gctgatgcgc atatcccgcc
     8401 gccgcgcggt cgcgtcggtg gcgaccgcct acgtcaccgt catgcgctgt gtaccgctca
     8461 ttctggtgct gttctggttc tatttcctgg tgccactggt catcggccgt cccatcggct
     8521 cgctggtgtc ggcactgatc gccttcgtca tgttcgaggc ggcgttctat tgcgagatcg
     8581 tgcgcgccgg cattctcagc atccgggccg gccagaccga ggccgggctg gccaccggca
     8641 tgcggcgctg gcagacgcta cggctcatcg tcatcccgca ggcggtgcgg gccatggtgc
     8701 cgctgctgct caaccagatc atcatcgtct tccaggacac ctcgctggtc tacgtggttg
     8761 ccctgacgga cttcatgact gcggccagcg tgctcgccca gcgcgacgga cgccccaccg
     8821 aggtctatac gttcgtcgcc atcgtctacc tcatcatctg cttcacgctt tccaagagcg
     8881 tcgcgtacta caaggcaaga gggtccaaat gatcgtgctc gacagcgtcc acaagtgcta
     8941 cgggcgcacc ccggtgctca acaactgttc cgccacggtg aagctgggcg aagtcgtggt
     9001 catctgcggc ccatcgggtt cgggcaagag cacgctcatc aaatgcgtga acggcctggt
     9061 gccgttcgac cggggcacca tcaccgtgga cggcatctcg gtgggcgaca agcgcaccga
     9121 cctgccggcg ctgcgcaccc gcatcggcat ggtgttccag aatttcgagc tctacccgca
     9181 catgacggcg ttgcagaacg tcagcctggc gcaaatccat gtgctgaagc gctcgcgcgc
     9241 cgaggccgac gcgcggtccg aacagttgct ggcgcgggtc gggctggcga acaagctgtc
     9301 cagccggccg gggcagcttt ctggtgggca gcagcagcgg gtgtcgatcg cccgcgcgct
     9361 ggccatggat ccgaaagcca tgttgttcga cgagccaacg tcggcgttgg atccggagat
     9421 ggtcaatgag gtgctggagg tcatgcaggc gctggccacc gagggcatga ccatgatggt
     9481 ggtcacgcac gagatggggt tcgccaagcg cgtggcggag cgcgtgctgt tcatggacgc
     9541 tggcgagatt cttgaagatt cgcccaaaga gcagttcttt gcggcgccgt ccactgagcg
     9601 ggcgcggcag tttctgacga aggtgcttag tcattgaagg ggttggttgc ggtgttttgg
     9661 tgttggggcg ttttggtgtt ggggcgtttt tgttcttcag gcggcccggg cgctgaccac
     9721 agaggcttta tatgcgagta agtattcttg gcgcgggcgc cattgctttt gggaccgccg
     9781 cctacctggc gcgggcgggg catgatccga tgctgtggtc gccgtcgggg cgccgcacac
     9841 gcgcgctggc gggtggcgcc aggctggtgg cgcgcaatgc agtggagggc gagttctcgc
     9901 cacgcgtggc cgcgtcttgt gccgaggcgg tcgcccaggc cgaggtggtc atgctggcgc
     9961 tgccaggtta cggtcacaag gcggtgctcg atgccgccgc gccgcatctg cgcgtgggcc
    10021 agccggtcat catcagttcg catctgtcgt tcggagcgct ttatctgtcg cgcctgctgg
    10081 ccgctcgcgg tgtgaaggcg ccgatcgtcg cctggggcac cacgctgacc actggtggtc
    10141 agcccagcct cacggaggcg ttcgtgtcga ccgtgcgtca gaaggtggat atggccacct
    10201 tgccgcacag ctatgccgat gaaggactgg cgctgtgcac cgcactcttc ggcgatcact
    10261 ttgtggcgcg tgaggggctg ctcgccatcg cgctgagcaa tctcaatccg cagaaccacc
    10321 tgggcatcgc catgctgaac ctcacgcgca tggagcgggg cgaagaatgg aaccagagcc
    10381 agaacgtgac cccggctgtg gggcgtctga tcgaatcact ggacgccgag cgcctggcca
    10441 tcgccgaggc cttctctttg tcggtcaaga cggtccggca gcatttctcg ctgtccttcc
    10501 acgttcccga agacagtgtg tcggccatga accaggacat gttccgtcgc gggcacagtg
    10561 gcctgggccc gaagacggcg gacagccgct acgtgctgga agatgtgccg ttcggtctgg
    10621 tgccgacggc gcgtctgggc cgtctggccg gtcgcccggc tcctctgcac gaggcgggca
    10681 tcgcgctgtt ttcggccgcc tacgggcggg acttcccagc ggaaaacaac ttgctgcccg
    10741 ggctggatct gcccgaacgg atcagcatcg aagcgctgat cgacctgacc cgcggcggct
    10801 attcaaaata gccgggccgc aatgtcgcat ggcgttgata ttcggacgat ccatccatcg
    10861 agttttcaat tcaggacgct acgcatcagc cgcgcaccaa ccccggcgca gcgacagtct
    10921 gcatgggatt gaacggcgtt tgccgcgctg tgtccaggca gtcgagcagc gcccggacgt
    10981 acggcgacag cgtgctattg cggcgtgtgc agatgcgcag cggccgtgtc gcccactcgt
    11041 ctgaaatggg caggatgcgc acgccttcgc aattcagcag ttgcaacacc gggcgcggca
    11101 tgacgcccac ccccactccg gcgcggatca tggacagcat cgaggcgaaa ctggccacct
    11161 ggatgcggat gtccaactgg cgccccagcg acgtggccat gttgacgatg aaggtgtaga
    11221 tggccgatcc gccctgggca cagacgaacg ggcgttccag acagtccgcg aaactgactt
    11281 cttcgcgagc ggccagggaa tcggtctcgg gcagcaccgc catcagctca ttctcgcgat
    11341 acggcatgaa atcaagttcg ggatggtggc cgtcccaggt cacgatgccc acgtcggcct
    11401 ggccggcggc caccgccgcg agggtttcat ggctcaagcg ctcggacatc acgatgcgca
    11461 cctcggggta gtcgcgcagg aatgactgca ggtcgtgcgg caggaaggat tccactgccg
    11521 tgctgctggc gaacaaggcc acgtgggcct tgatcccctt ggcgtagggg gccagatcgg
    11581 cgtgcatctg ttccagttcg gccaggcagg cccgacagtg ctgcagcatg acctggcccg
    11641 cgctggtcag cgagacgccg cgcgcctcgc gtttgaacag ttgcgcaccc agggtttcct
    11701 cgagctgctt gatgcgcagg ctggccgagg acggcgccag aaaacacgcc gccccaccgc
    11761 gggagatgtt ccccgcgtcg gcgatggcca cgaagagccg caaatcgatc aggtcgtacc
    11821 gcatgaatgt ttgtatataa cgaaagctac tttctaaata acccgcttgc cgccggtatt
    11881 cacgtgtgaa agactgtctt ccgaaacagg ataccgtaaa aggcggttcg ccaacggcaa
    11941 accgtcagtc gaaattcaga aacggctcga tccgtgcccc gtgcctcccg gcccgcacgg
    12001 cccgcccccc cacaagacac ggatacgaac catgctcatg acacccacga cacccacgac
    12061 ggccacgctc cccatcggat tgtccgccct ggagcgccgc ctggcgcaag acctggcttg
    12121 gctggacctg ccggccaggc aatgggttgc gccgggatcg gtgaacggcc aggcgttgct
    12181 cgacgtcgcc atcatcggtg gcggcatggc ggggctggcg gcggccaccg ccatgaccca
    12241 catgggtatt cgcgcacgca tctacgacaa agcgccgcaa ggattcgaag gtccgtgggc
    12301 caccaccgcc cgcatggaaa ccctgcgctc gcccaagcag ctcaccggcc ccgcgctggg
    12361 cctgccatcc ctgacgttcc gcgcctggtt cgaagcccag tatggcatcg cggcctggga
    12421 tgcgctggac aagatcccgc gtctgcaatg gatggactat ctgcgctggt accgtcgcgt
    12481 catgaacctg gatgtccaca acgagcatca cgtcgacagt atcgttccac aggccgacca
    12541 cgtgcggttg cgtctccaga cgccgtccgg tccacaacgc gtgctcgcgc gccatgtcgt
    12601 gctcgccacc ggacgcgcag gcctgggggg tccgttcatt cccggcttcg tcgaaaacct
    12661 cgatcggcga ctctgggcgc attcgtccga cgagatggac tacgccgcgc tgcgcggcaa
    12721 gcggatcggc gtggtgggcg cgggcgcctc ggccatggac agcgccgcca cggcgctgga
    12781 aaacggcgcc gcgcacgtcg acctgatcat ccgccggccc gacctgcccc gcgtcaacaa
    12841 gggcaagggc gccggcagcg ccggtttcac caacggctat gtgcatctgc ccgacgattg
    12901 gaaatggcgc atccgccact acatcaacgt gcagcaggtg ccaccaccgc gcgccagcgt
    12961 gctgcgggtc tcgcggcacg acaacgccag cttcaacctg ggttcaccca tactcgacgt
    13021 ggccccacgc ggtgggcgcc tcgtgctgcg cacaccgcgc aagaccttcg atctggactt
    13081 cctcattatc gccacgggct tccgcgtcga ctggcaggcc cgcccggaat acgccggcat
    13141 cgcgccgcac gtgcgcctct ggggtgatcg ctaccagccc gaacccggcc aggaagacaa
    13201 ggaactccac gactcacccg atctgggccc ggttttcgaa ttccaggaaa agccggagaa
    13261 tccgctgccc ggtctgtcgc gtgtccactg cctctgctac ccggcctcgc taacacacgg
    13321 cacggtctcg ggcgacatcc ccgccgtgag cgacggcgct ctgcgcctgt cacggggcat
    13381 ggccagcctg ttgttcaacg agaacatcga tcaccattac cgcgacctgc tggcgtatga
    13441 cgaacccgaa gtgttcggcg acgaatggac accggcggcc tgagggccgt taccgccagc
    13501 actataatga ccgacggtgt ggtgctgttg cccgtcacga cacctgagca ggccgaagtg
    13561 ggattcgccc gcgatttggg gcctttcgta tgaataggcc ctagcctctg gctggctgcc
    13621 caactttcgg gccatatccc ggaacgaacc ctggtgtccg ctccggtctc aggagttcgc
    13681 atgacgaaac gtgcctccgc acacccgacg cggtcagtca tcgcgtccgg cagcccgggt
    13741 acggtgccta tcgcaccagc cctcgacacc accatggaag acgcccggat cacccggggc
    13801 tcgaccacgc tggggcgcag ggtgctctgg atcagccttc tggccatcat tctgggcgcc
    13861 atcgccgcct tgatcgccca gttgctgatg ttcatgatca atatcatcac ggacgtcacc
    13921 ttctacgggc gcatttccga cgtcgtcggg ccagccgccg ccggacgcac cggttcgttc
    13981 gtcctgtcgc ccgcccacaa ccagttgggc ctgtgggtga tcctgatgcc cgccatcggc
    14041 ggactgatcg ccggcatcat ggcccgctgg ggttcacgcg ccatccaggg ccacggcatc
    14101 cccgaggcca tggagcagat cctgcagaat gaatccaaca ttccaccccg cgtcacctgg
    14161 ctcaagccgg tttcgtccgc cttcgcgatc ggcaccggcg gcccgttcgg cgctgaaggc
    14221 cccatcattt ccactggcgg ggcgctcggc tcgctgatcg gccagctcct gtccgtcacc
    14281 gccaccgaac gcaagaccct gctggccgcc ggcgccgccg ccggcatggc ggccgtgttc
    14341 ggcgcaccag tgtcatcgct ggtgatggcc atcgagctcc tgctcttcga actgcgtccc
    14401 cggtcgctga tcccggtcgc actggcggcc gtcacggcaa ccggcgtgcg ctacgccacc
    14461 tacgggccgg gcgcggtgtt cgc
//
LOCUS       DASTNK010000087         3339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_802258, whole genome shotgun sequence.
ACCESSION   DASTNK010000087 DASTNK010000000
VERSION     DASTNK010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802258"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..553)
                     /locus_tag="VFR20_06780"
     CDS             complement(287..553)
                     /locus_tag="VFR20_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU0230257.1"
                     /translation="MTTLTITSRSQDTFRKEVLQHLGVALGSQVEVNLLPNGRAELKA
                     AKSKGSAREPQSFLKGKTNDTKLIIKEINDAIADTNVWPRSTRC"
     gene            complement(623..1249)
                     /locus_tag="VFR20_06785"
     CDS             complement(623..1249)
                     /locus_tag="VFR20_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEU0230258.1"
                     /translation="MLTTEICLSFFGIAVLLALAPGPDNLFVLMQSALWGPRAGLFVV
                     FGLCTGLVGHTLAVAVGLAAVFAASQTAFTVLKLVGAVYLAYLAWGAFRAHGQMMQGE
                     KLPRRTGGELYRRGIIMNLTNPKVSLFFLAFLPQFTSPDRGSVAMQTVYLGLLFMLAA
                     LLVFGSIAFFSGAFGTLLQRSVRAQKILNRTAGTVFLGLALKLAFMHR"
     gene            1391..2593
                     /gene="metC"
                     /locus_tag="VFR20_06790"
     CDS             1391..2593
                     /gene="metC"
                     /locus_tag="VFR20_06790"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEU0230259.1"
                     /translation="MANTDSPARLETLLQHTGTAEFDPKTGTAPVSIPSMRTSTVRFA
                     DLDALARAQAAKARGERVVTYGRVGMDTHAALEKVFCHLEGGQRCFLAPSGMAAITVA
                     LMSLLNTGDHVIVADCVYGPVRNLDKTVFTRMGIDVTYASADPKTLAGLVRDNTRVVY
                     VESPGSLLFEMLDLPALAAFTRERGLILATDNTWGSGYIYQPLALGADVSIIAGTKYV
                     GGHSDLMLGAVVVNDAGLARRINDTHYAMGYSISADDAWLALRGARTLPVRMREHAQQ
                     ALRVCEFLAARPEVTRIYHPAWPADAGHALWRRDCTGSNGMLSVELKLDDAAAHRFVN
                     ALRLFGIGFSWGGFESLVQLVDKAAVAGHSYWKDQGKDQALVRLHVGLEAVDDLIADL
                     AQALDHAA"
     gene            complement(3009..>3339)
                     /locus_tag="VFR20_06795"
     CDS             complement(3009..>3339)
                     /locus_tag="VFR20_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEU0230260.1"
                     /translation="IGRTDLHDKVFAAIHEENKPIYDEKAILDWIATQGVDRAKFKAV
                     FDSFGVQTKVTRANELAKNYKIDGTPSFAVDGKYVTSPAMTNGYESSIEETQRLVDMV
                     RKQSQKK"
BASE COUNT          614 a         1147 c          962 g          616 t
ORIGIN      
        1 gccggaacct gtaatgtctt caacttcttc ggctattcag tctagctcat tgggaaatgc
       61 gcgctggccg gaactgcgcc gtcagtggcg tttcggccag aagcagtcta gctcattggg
      121 aaatgcgcgc tggccggaac agacactttt tcttgtcgcg gtagagaagc tgggccggct
      181 tgcccaagcg gtggggcagg ccacccgtcc gcgaaatcgc cgcaggcatc gagcatcgcc
      241 agaccggtat cgacggcccg gcggttggct actatgtctc tcgtgttcag cagcgtgtgg
      301 atcgcggcca cacgttggtg tcggcgattg cgtcgttaat ctccttgatg atcaacttgg
      361 tgtcgttggt cttgcctttc aagaagcttt gcggctcgcg ggctgaacct ttggatttgg
      421 ccgctttcag ctcggcccgg ccatttggca gcaagttgac ctcgacctgg ctacccagtg
      481 ccacgcccaa atgctgaagc acctctttcc gaaacgtgtc ctggctccgc gaggtgatgg
      541 tgagtgtggt catggcaata cctctttggt cgcggcaata caaggcagtt ttatcttgct
      601 ttatgacgcg tggtgccatc catcaccgat gcatgaacgc cagcttcaac gccagcccca
      661 gaaacaccgt ccctgccgtt cgattcagaa tcttctgcgc ccgcaccgag cgctgcaaca
      721 acgttccgaa cgccccagaa aagaacgcga tgctcccgaa caccagcagc gccgccagca
      781 tgaacaacag ccccagatac acggtctgca tcgccactga cccacgatcg ggtgacgtga
      841 actgaggcag gaacgccagg aagaacagtg acaccttcgg gttcgtcagg ttcatgatga
      901 tcccgcgccg atacagctca ccaccagtgc gccgcggcag cttttccccc tgcatcatct
      961 gcccatgtgc ccggaacgcg ccccaggcca gatacgccag atacaccgcc cccaccagct
     1021 tcagcaccgt gaatgccgtc tgggaggccg cgaacaccgc cgccaggccc accgccaccg
     1081 ccagcgtgtg ccccaccagc cccgtgcaca acccgaacac cacgaacaac cccgcgcgcg
     1141 gcccccatag cgccgactgc atcagcacga acagattgtc cggcccgggc gccagcgcca
     1201 gcaataccgc gataccgaag aacgacaggc agatttccgt ggtcagcatg atatggtcgt
     1261 atgatggatg ccttggtcgg cgcggcgagt gtttcgtttg aatggcttgc gcacttgcac
     1321 tcgagcgcac tggatgcctg ccgccgcccg tagttccatc tgtttttacc acacaacaaa
     1381 accccgcgac atggccaaca ctgactctcc cgcccgcctc gaaaccttgc tgcagcacac
     1441 cggcaccgcc gaattcgacc cgaaaaccgg caccgcgccc gtctccatcc cctccatgcg
     1501 gaccagcacc gtccgcttcg ccgacctcga cgctctggcg cgcgcccagg ccgccaaggc
     1561 gcgcggtgaa cgcgtcgtca cttatgggcg cgtcggcatg gacacccacg ccgcgctgga
     1621 gaaagtgttc tgccatctcg aaggcgggca gcgctgcttc ctcgcaccct cgggcatggc
     1681 ggccatcaca gtcgccctga tgagcctgct caacaccggc gaccacgtca tcgtggccga
     1741 ctgcgtctac ggccccgtgc gcaacctgga caagaccgtg ttcacccgca tgggcatcga
     1801 cgtcacctac gcctccgccg atccgaaaac cctggccggg ctcgtgcgcg acaacacccg
     1861 ggtcgtgtac gtcgaatccc cgggctccct gcttttcgaa atgctcgacc ttcccgcgct
     1921 ggcggcgttc acccgcgaac gcgggctcat cctggccacc gacaacacct ggggctctgg
     1981 ctacatctat caaccgctcg ccctgggggc ggacgtctcc atcatcgccg gcaccaagta
     2041 cgtgggcggg cactccgacc tgatgctcgg ggccgtcgtc gtcaacgatg ccgggctggc
     2101 ccgtcgcatc aacgacacgc attacgcgat ggggtattcg atcagcgccg atgatgcctg
     2161 gctggccttg cgcggcgcgc gcacgctgcc ggtgcgcatg cgcgaacacg cccaacaggc
     2221 cttgcgcgtc tgtgaattcc tggccgcgcg gcccgaggtc acccgcatct accaccccgc
     2281 ttggccggcc gacgccgggc acgcgctctg gcggcgcgac tgcacaggct ccaacggcat
     2341 gctgtcggtt gagctcaagc tcgatgacgc ggcggcgcac cgtttcgtca acgccctgcg
     2401 tctgttcggt atcgggttct cgtggggtgg ctttgaaagc ctggtccagc tcgtggacaa
     2461 ggcggccgtg gccgggcaca gttattggaa ggaccagggc aaggatcagg cgctggtgcg
     2521 tttgcacgtg ggcctggagg cggtggacga cctgatcgcc gacctggcgc aggcactgga
     2581 tcacgccgcc tgagcatcaa gccaccctga tgtttccgat tcatttgcca acagccccac
     2641 gcgaagggcg tcgttgacgt attcgttgag cgccagccat tggacgctct gtgaacaacc
     2701 accgctctgc gtgcaacaac ccacgccagc gcaaggaccg ggcagggtag ccaacagtgg
     2761 gccgactttg tcgcaagccg gccaatcacg ccagcgcggg gaggcaaggg agcgactgtt
     2821 tgagcggggc gcctgcgatc agcccagtgg gctgatcgcc ccgcgagttt cgcgaccgcc
     2881 ccgcgctggc gtgattggcc gggaagcccg gcgcagccgg gccgcagcga cggaggccca
     2941 ctgttggcta ccctgcccgg tccgactaaa gaaccaagca atcatctcaa gccggcagaa
     3001 ctcaaacgct acttcttctg actctgcttg cgaaccatat ccaccagccg ctgcgtttcc
     3061 tcgatcgaac tttcataacc gttcgtcatg gccggcgacg tcacatactt gccatccact
     3121 gcgaaactcg gcgtcccgtc gatcttgtag ttcttcgcca actcattcgc gcgcgtcacc
     3181 ttggtctgca caccgaaaga atcgaacacc gccttgaact tggcgcggtc cacaccctgc
     3241 gtggcgatcc agtccagaat ggccttttcg tcgtagatgg gcttgttttc ttcgtgaatg
     3301 gcggcgaaca ccttgtcgtg cagatcggtg cggccgatc
//
LOCUS       DASTNK010000088        10704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_433942, whole genome shotgun sequence.
ACCESSION   DASTNK010000088 DASTNK010000000
VERSION     DASTNK010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10704
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433942"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VFR20_06800"
     CDS             <1..254
                     /locus_tag="VFR20_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU0230261.1"
                     /translation="RAFNPVPGATMTLPGLADPVKVWMAQALDGPARQAPGGIESVSA
                     QGVDVATADGVLRLLELQKAGGRRQPVAAFVQGWQTQAD"
     gene            complement(359..1675)
                     /gene="rimO"
                     /locus_tag="VFR20_06805"
     CDS             complement(359..1675)
                     /gene="rimO"
                     /locus_tag="VFR20_06805"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEU0230262.1"
                     /translation="MNSPKVGFVSLGCPKALVDSERILTQLRTEGYAITPDYDNADVV
                     VVNTCGFIDSAKAESLDAIGEALAENGRVIVTGCMGVDESAIRQVHPSVLAVTGPQQY
                     EQVVRAVHDAAPPNLQHDPYVDLVPPQGVKLTPRHYAYLKISEGCNHRCSFCIIPSMR
                     GNLVSRPIGDVMGEARRLVDAGVKELLVISQDTSAYGVDVKFRTGFWDGRPIKTHMTQ
                     LCEALSAFGVWVRLHYVYPYPHVDEVIPLMADGRLLPYLDIPFQHASPRILKAMKRPA
                     FEDRTLARIKTWRETCPDLTLRSTFIVGFPGETEDDFQYLLDWMSEAQLDRVGCFQYS
                     PVQGARANELPDQVPDDIKQDRWERFMAHQQAISVERLARKVGTEIDVLIDEVGDDQI
                     VGRSSGDAPEIDGNVFVSSERPLQPGQMIRARVTESDEYDLYAEVV"
     gene            1821..3182
                     /gene="rsmB"
                     /locus_tag="VFR20_06810"
     CDS             1821..3182
                     /gene="rsmB"
                     /locus_tag="VFR20_06810"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEU0230263.1"
                     /translation="MPDNSSPPPRPPLSDILLDSAQCVRAVSSGRSLSDALAEVARES
                     RPAVQAVAFHVMRRLGLARAVRGILVRRTPPDALADALLLVALAVLDTALDHAQPDPD
                     AIPARRDLPVYAVHTVVDQAVRAATAHRATRPYKALVNASLRRFAREQRSILAQAAQS
                     DEARWNYPEWWLRLVREAYPDNWRALLEAGDRPGPMTLRVNTRRSSVGDLLPRLRDAG
                     LEARALDGRAVTLDTARPVRAVPGFDEGWWSVQDISAQRAAALLAPVAGERVLDACAA
                     PGGKTAHLLELADVRLLALDDDATRLARIRENLDRLGLMSDAVSLRCADAADVDAWWD
                     GTPFDAVLADVPCTASGIVRRHPDIRWLRQSQDVVATARLQERIVDALWRVVGPGGRL
                     VYATCSVFPQEGVRQAARFRKRHPDATSLPAPGQLLPIGSPDVGDGFFYAAFAKRTPD
                     GQK"
     gene            3218..3802
                     /locus_tag="VFR20_06815"
     CDS             3218..3802
                     /locus_tag="VFR20_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEU0230264.1"
                     /translation="MIFRLMCAFSICLLLAGPTPPVSANGAVNQVVRVDPVVRQGKLY
                     VDADVDLTVNHELRNVAEKGVPVYFTADLSIVAERWWWFDKTVVDKQRTWRIVYNALT
                     RQWRVGTSDLTMPEPSFDDAMALVRHIRDWAVANTSELEPGKKYKGRIRVRLDTSLLA
                     RPFQIDAFNSSAWSLSTPWKDFTFSVTTGTADPS"
     gene            3799..6135
                     /locus_tag="VFR20_06820"
     CDS             3799..6135
                     /locus_tag="VFR20_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0230265.1"
                     /translation="MRWGLRLLLAIAVVSALALLALLVWSTGNASRYAQQYDTLLMLN
                     GVLAVALILWVVVLAGRLMRQIRRRQFGARITARFALSFALIGVLPGALIYVLSVQFM
                     SRSIESWFNVRVDTALEAGLNLGRAALDSQIADLTSRARAIAASLETTDDADKAIAIT
                     RLREANGIAEAMVFTGNGRLIAFSSSVYGQLLPALPPSNVMNQLRVSNGYSAAETDEP
                     GFSQSQSGGGTGLRLRVIIPLNSASSFSSLMGVSPDRRWLQVIQAVPERIAHNANLVQ
                     NGFRNYQELALSRLGLRKLYGITLTLALILAVFAAMVVALAISRRLVRPLLTLAAGTQ
                     AVGVGDYRPLPEPPAKDEVGQLTRSFNAMTRQLDEARQMVEKNRQQLERSNVYLETVL
                     SNLSSGVLVFDEMFRVTMFNEGAQSILHLDLRSVIGRPLETIDAALEWVRLVRAAFAE
                     HAAVGSERLYWQKQFELEIERADDNEKKMHAITLLARGTHLSVDGRGNGYLVVFDDIS
                     EVISANRVVAWGEVARRLAHEIKNPLTPIQLSAERLAMKLDGHLNDADAAILARSTNT
                     IVNQVASLKKMVDDFREYARTPPAQMERIDINALIAEVLTLYGWDPAGGAVRDDGQSV
                     KIEAEFDQEVPAIEGDPTQLRQLTHNLLANARDSAVQARTLAGARIYVQTRLMHSGTN
                     SGGDLPAVRLTVSDNGAGFASQVLSRVFEPYVTTKVTGTGLGLAIVKKIVEEHGGRVD
                     ISNRKEGGARVSILLTRLAHQQSAMDEEAQANDNAQKR"
     gene            6146..6832
                     /locus_tag="VFR20_06825"
     CDS             6146..6832
                     /locus_tag="VFR20_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0230266.1"
                     /translation="MARILVVDDEVGIRELLSEILYDEGHTVELAENAGQARASRLRV
                     RPDLVLLDIWMPDTDGVSLLKEWSSQGLLDMPVIMMSGHATVDTAVEATRIGAVDFLE
                     KPITLQKLLKTVAAGLARPVVSPALMAAARMPAGMRAEQDKPLTTVPMPEMATAPEPD
                     NSLLGSIALDQPLRDARDEFERIYFEYHLGRENHSMTRVSEKTGLERTHLYRKLKQLG
                     IDSSRKKNNS"
     gene            complement(6874..7803)
                     /locus_tag="VFR20_06830"
     CDS             complement(6874..7803)
                     /locus_tag="VFR20_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0230267.1"
                     /translation="MSQPPTLPLSPAHVIQQFSRRSPLDAAQFLYGEVAQRMLQRLSY
                     IRVAPKSVLDAGCGAGHAIEPLRSRYPEMDYTGLDSCAALLDVARQRYTEKPGLWNKL
                     RNKPARAVRFVEADMAASGLPDESLDLIWSNMALHWHPRPHDVLSEWRRTLKPGALVM
                     FSCLGPGSLKNLREALTAADIHTATPAFVDMHDFGDLLIQRGFSDPVMDQEILTLTYR
                     EAGKLLDDIRDLGGNPSLARQRGLAGRAWKQRLTAALEKQRHMDGTIHLELEIAYGHA
                     WRSMPRKVIGRPAAPVDASGHRPRVRPRDETDS"
     gene            7885..8676
                     /locus_tag="VFR20_06835"
     CDS             7885..8676
                     /locus_tag="VFR20_06835"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU0230268.1"
                     /translation="MAYPEGVRESVSATLARLARCAGVSRRWVESKVDALPSDCLLCR
                     GRARAGRLCPDCLAEVSATMRDGRRRCAICALSLEGLDTCPDCAAMPPSFDRVLAAFD
                     YEFPGDLLIHQLKETRRFTCARVLSGILAQRIHVDPAGLPHHTILVPVPASRASVRKR
                     GFNPAAEVARSLAWRLGLACRPALLRRVREGVRQASLSRGERIRNARGLYECTGRVDG
                     YDIAVVDDVLTTGSTAHGIARAFKDSGAASVSVLVLARTRHHFSS"
     gene            8687..9169
                     /locus_tag="VFR20_06840"
     CDS             8687..9169
                     /locus_tag="VFR20_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU0230269.1"
                     /translation="MFHIVLVAPEIPPNTGNVIRLAANTGARLHLVRPLGFELDDRKL
                     KRAGLDYHEWADMRVHDTLEKAFSHIGGDPARRFAITTRGARHIDALTFQAGDVFVFG
                     RETAGLSAEQMAWFPDGQRIRLPMMPGQRSLNLSNAVAVTVYEAWRQEGYRGAVGIDA
                     "
     gene            complement(9229..10224)
                     /locus_tag="VFR20_06845"
     CDS             complement(9229..10224)
                     /locus_tag="VFR20_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0230270.1"
                     /translation="MKAFSRSRRIWHKTALGLALAAATFSAPSFAGTWPDKPLHMIVP
                     FPPGSSPDILARVIADPLGKTLGQHVIVENKPGAGGNIGTREATRAKPDGYTILMTIN
                     GPMVTAPKLYKKTLGYKPDQLTPITLIGTSPNVLIVPQNSPAKTVSEFVALAKAKPDS
                     LNFGTVGPGSSSHLCMAMLEHKADIKLQQIPYAGFPEVVRSVVSGDIQASFMVPGIAM
                     PQIQAHKVRALAITSLKPSDILPGIPTMASQGYPGFESISWDAMFVPAGTPKDIVARL
                     NTAITQILRRPDVKKRMEALYFSPAPSTPEQLTALIKSDTVKWGGLIDRLHLSRD"
     gene            complement(10262..>10704)
                     /locus_tag="VFR20_06850"
     CDS             complement(10262..>10704)
                     /locus_tag="VFR20_06850"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU0230271.1"
                     /translation="LKNIMAIACGIADGLGLGTNARAALITRGLAEMQRLGVALGGQA
                     DTFMGLTGLGDLVLTATGPLSRNRQAGLAIGQGQDPRQVLASGTTIEGARCAGAALAL
                     GRAHHIDLPITHAVCRVLFDGMAPHQAVAGLLARQARTEAASPP"
BASE COUNT         1780 a         3246 c         3638 g         2040 t
ORIGIN      
        1 tacgggcatt caaccccgtg ccgggtgcca ccatgacgct gccgggcctg gccgacccag
       61 tgaaggtctg gatggcgcag gcgctggatg gcccggcccg gcaagccccc ggcggcattg
      121 agtcagtgtc ggcccagggc gtcgatgtgg cgacggccga tggcgtgctg cgtctgctgg
      181 agctgcagaa ggcggggggg cggcgccagc ctgtcgctgc cttcgttcag ggttggcaga
      241 cacaggcgga ttagtcagcc tggggcccgt gctcgtctgg cctgactaag aagccaggcc
      301 aaaggtactg tgcccgcaga acttaaacga cgaatgcggc cacttcaacc aaaacgccct
      361 acaccacctc ggcgtaaagg tcgtattcgt ccgattccgt cacacgcgcc cggatcatct
      421 ggcccggttg cagcgggcgt tcgctgctga cgaacacatt cccatcgatt tcgggcgcgt
      481 cgccgctgga gcggcccacg atctggtcgt caccgacctc gtcgatcaac acgtcgattt
      541 ccgtccccac tttgcgcgcc agccgttcga cggagatggc ctgctggtgc gccatgaagc
      601 gttcccagcg gtcctgcttg atatcgtcgg gcacctggtc gggcagttcg ttggcgcgcg
      661 cgccctgtac cggcgaatac tggaaacagc cgacgcgatc gagctgggcc tcggacatcc
      721 agtccagcaa gtactggaaa tcgtcttcgg tttcccccgg gaaccccacg atgaaggtcg
      781 agcgcagggt caggtcgggg caggtttcgc gccaggtctt gatgcgcgcc agcgtgcggt
      841 cttcaaaggc cggacgcttc atggccttga ggatgcgcgg gctggcatgc tggaacggaa
      901 tgtccaggta gggaagcagg cggccgtcgg ccatcagcgg gatgacctcg tccacgtggg
      961 ggtacgggta cacgtagtgc aggcgcaccc agacgccgaa cgcggacagc gcctcgcaca
     1021 gttgcgtcat gtgggttttg atggggcgtc cgtcccagaa acctgtgcgg aatttgacat
     1081 ccacgccgta ggcgctggtg tcctgcgaga tcaccaatag ttctttgaca cccgcgtcga
     1141 ccaggcggcg ggcctcgccc atgacgtcgc caatggggcg gctgaccaga ttgcctcgca
     1201 tcgatggaat gatgcagaag ctgcagcgat ggttgcagcc ttcggagatc ttgaggtagg
     1261 cgtagtggcg cggggtcagc ttgacgcctt gtggcggcac caggtccaca taaggatcgt
     1321 gttggagatt gggcggcgcg gcgtcatgca ccgcacgcac gacctgttca tactgttgtg
     1381 ggccggtcac ggccaggacg gacgggtgga cctggcggat ggccgattcg tccacgccca
     1441 tgcagcccgt gacgatgacc cggccgtttt cggccaacgc ctcgccgatc gcgtccaggg
     1501 attccgcctt ggcgctgtcg atgaacccgc aggtattgac gactacgaca tcggcgttat
     1561 cgtagtcggg ggtgatggcg tagccttcgg tgcgcaactg ggtgaggatg cgctcggagt
     1621 cgaccaacgc cttggggcaa cccaggctga cgaaaccgac tttgggggaa ttcatggaaa
     1681 agacctctgg tagtcttgtg gttgggtgaa gagaaacgga ttgtgacgac gagggtggtt
     1741 cggtccaccc atgggggtta cccgatgatt tgatgataaa gtcgcattca aaatcatgat
     1801 tccagcaggc cagacacctc gtgcccgata attcctctcc gccgccccgc ccgcctttgt
     1861 ccgacatttt gctggacagc gcgcagtgcg tgcgggcggt gtcgtcgggc cggtcgttgt
     1921 ccgacgcgct ggccgaggtg gcgcgggaat cgcgcccggc ggtccaggca gtggcgtttc
     1981 acgtcatgcg gcgcctgggg ctggcacgag cggttcgcgg cattctggtg cgccgcacgc
     2041 cgcccgatgc cctagccgat gccttgctgc tggtagcgct tgccgtgttg gacacggcac
     2101 tcgaccacgc ccagcccgac ccggatgcca tccctgcgcg gcgcgatctg cctgtgtacg
     2161 ccgtacacac cgtggtggac caggcggtga gggcggcaac ggcgcatcgg gccacgcggc
     2221 cctacaaagc actggtcaac gcctcgttgc gccgattcgc gcgtgagcaa cgttctattt
     2281 tagcccaggc tgcgcaaagt gacgaagccc ggtggaatta cccggaatgg tggctgcgtc
     2341 tggtgcgcga ggcctatccg gacaactggc gggcgcttct cgaagcgggg gatcgcccgg
     2401 gtcccatgac cttgcgtgtc aacacccgcc gctcctctgt gggtgatttg ttgccgcgtt
     2461 tgcgtgatgc tggcctcgag gcgcgggcgt tggatgggcg ggcggtcacg ctcgacacgg
     2521 cgcggccggt gcgggccgtg ccggggttcg acgagggatg gtggtctgtg caggacatct
     2581 ccgcgcaacg ggccgccgca ttgctggccc ccgtggcggg cgagcgtgtg ctcgacgcct
     2641 gcgcggcgcc tgggggcaag accgcgcatc tgctcgaact ggccgacgtg cggctactgg
     2701 cgctggatga cgatgccacc cgcttggccc ggatccgcga gaatctcgat cgtctggggt
     2761 tgatgtccga tgcggtctcg ctgcgctgcg ccgacgccgc cgacgtcgat gcgtggtggg
     2821 acggaacacc gttcgacgcg gtgctggccg acgtgccttg cacggcttcc ggcatcgtgc
     2881 gccgtcatcc ggacatccgc tggctcaggc agagccagga tgtcgtggcg acggctcgtt
     2941 tgcaggaacg cattgtcgac gcgctgtggc gcgtggtcgg gccgggtggg cgcctggtgt
     3001 acgcgacgtg ttcggtgttc ccccaagagg gggtgcgtca ggccgcccgg tttcggaaac
     3061 gccaccctga cgccaccagc cttccagcgc ccgggcagtt gctgcccatc ggttcgccgg
     3121 acgtcggcga cgggtttttc tacgccgcat ttgcaaagcg gacgccggac ggtcaaaaat
     3181 agggctcgag aagagtacga gggagtgcgg cattgcaatg attttccgat tgatgtgtgc
     3241 tttctcgatc tgtctgctgc ttgccgggcc gacgcctccc gtgtcagcca acggcgcggt
     3301 caatcaggtc gtgcgtgtcg acccagtcgt ccgccagggc aaactgtacg tcgatgccga
     3361 tgtcgacctg accgtcaacc acgaactgcg caacgtggcg gagaaaggcg tcccggtgta
     3421 tttcaccgcc gatctcagta tcgtggctga acgctggtgg tggttcgaca aaaccgtggt
     3481 cgacaaacag cggacgtggc gcatcgtcta taacgccctg acacgccagt ggcgcgtcgg
     3541 caccagcgat ctcaccatgc cggaacccag tttcgacgat gccatggcct tggttcgcca
     3601 catccgcgac tgggccgtgg ccaacacgtc cgagcttgag cccggcaaga aatacaaagg
     3661 ccgcatacgg gtgcggctcg atacctcact cctggcgcgg ccgtttcaga tcgacgcctt
     3721 caacagtagc gcctggtccc tgtcgacccc atggaaagat ttcacatttt cagtcaccac
     3781 cggcacagcc gatccatcct gaggtggggc ctgcgcctgc tgctggccat cgcggtggtc
     3841 agtgctctgg cgttgctggc gctgctggtg tggtcgacgg ggaacgcctc gcgctacgca
     3901 cagcagtacg acacgttgct gatgctcaac ggtgtgctgg cggtggcgtt gattctttgg
     3961 gtggtcgtgc tggcgggacg cctgatgcgg caaatacggc ggcgtcagtt cggggcgcgc
     4021 atcaccgcgc ggttcgcgct gtcgttcgcc ctgattggtg tgctgcctgg cgcgctcatt
     4081 tacgttctat ccgtgcagtt catgtcgcgc tcgatcgaat cctggttcaa cgtcagggtc
     4141 gacaccgcgc tggaggcggg gctcaatctc gggcgcgccg cgcttgactc ccagattgcc
     4201 gacctgacct cgcgggcgcg ggcgatcgcg gcgagcctgg aaaccaccga cgatgcggac
     4261 aaggccatcg cgattacccg cttgcgcgag gccaatggca tcgccgaagc catggtgttc
     4321 accggcaacg ggcgcttgat cgcgttttcg tcgtccgtct acggccagtt gctgccagcg
     4381 ctgccaccat ccaatgtgat gaaccagttg cgtgtttcga acgggtattc ggcggcagaa
     4441 acggatgagc cgggattttc ccagtcgcaa agcggaggcg gtacgggctt gcgcctgcgc
     4501 gtgatcatcc ccctgaattc ggccagttcg ttctcatcgt tgatgggcgt atcgccggat
     4561 cgacgatggc tgcaggtgat ccaggccgtg cccgagcgca tcgcccacaa cgccaatctg
     4621 gtccagaatg ggttccgcaa ctatcaggag ctggcgctgt cgcggctagg cctgcgcaag
     4681 ctctacggca tcacactgac gctcgcgctc attctggcgg tattcgccgc catggtggtg
     4741 gccctggcga tatcgcgtcg cctggtgagg ccgttgctga cactggcggc gggcacccag
     4801 gcagtgggcg tgggcgatta ccggccactg cccgagccgc cggccaaaga cgaggtcggg
     4861 cagttgacga ggtctttcaa cgccatgacc cggcaactgg acgaggcccg ccagatggtc
     4921 gagaaaaacc ggcagcagct tgaacgctcg aatgtctatc tggaaaccgt gctgagcaat
     4981 ctgtcctccg gcgtgctggt cttcgacgag atgttccgcg tgaccatgtt caacgaaggg
     5041 gcgcagtcca ttctgcatct ggatctgcgt tcggtgatcg ggcgcccgct ggagaccatc
     5101 gacgccgcgt tggaatgggt tcggctggtc cgcgccgcgt tcgccgaaca tgcggcggtg
     5161 ggatccgaac gtctctactg gcagaagcaa ttcgagcttg aaatcgaacg ggcggacgac
     5221 aacgaaaaga agatgcatgc catcacgctc ttggcgcgtg gcacgcatct cagcgtggac
     5281 gggcgtggca acggctatct ggtggtgttc gatgacatca gcgaggtcat ttcggccaat
     5341 cgtgtcgtgg cctggggcga ggtggcccgg cggctggcgc acgagatcaa gaacccgctc
     5401 actcccattc agctatcggc cgagaggctg gccatgaagc tcgatggtca tctgaacgat
     5461 gccgacgccg ccatcctggc gcgctcgacg aacacgatcg tcaatcaggt cgcgtcgctc
     5521 aagaagatgg tggacgattt tcgcgagtat gcccgcacgc cccccgcgca gatggaacgc
     5581 atcgacatca atgcgctgat cgccgaggtt ctgacgctgt atggctggga cccggcgggt
     5641 ggcgccgtgc gcgatgacgg gcaaagtgtc aaaatagagg ctgagtttga tcaggaggtg
     5701 ccggcgatcg agggcgatcc cacccagttg cggcaattga cgcacaacct cctggccaac
     5761 gcccgcgact cggccgttca ggcccgcact ctggctggcg ccagaatata cgtacagacg
     5821 cggctcatgc attccggcac caacagcggc ggggatctgc ccgctgtccg actgacggtt
     5881 tccgacaatg gcgcggggtt cgcgtcccag gtgctgtcac gtgtgttcga gccctatgtc
     5941 acgaccaagg tgacgggaac gggcctgggg ttggccattg tgaagaagat cgtcgaagag
     6001 cacggtggcc gcgtcgacat ctcgaatcgg aaggaaggcg gggcgcgggt ttcgatactg
     6061 ttgacgcgcc ttgcgcacca gcagtctgct atggacgaag aggcgcaggc gaacgataat
     6121 gcgcaaaaaa ggtaggtgac gctttatggc cagaattctg gttgttgacg acgaagtggg
     6181 cattcgcgaa ctgctctcgg agattctcta cgatgaaggg catactgtcg agctcgcgga
     6241 aaacgccggg caagcccgtg cgtcgcgcct gcgtgttcgc cccgatctgg ttctgctcga
     6301 catctggatg ccggataccg acggggtcag tctgctcaag gaatggagct cccaggggct
     6361 gctcgacatg ccggtcatca tgatgagcgg ccacgccaca gtcgacaccg ctgtcgaggc
     6421 cacacgcatt ggcgcggtgg attttcttga aaagcccatc accttgcaga agctgctcaa
     6481 gacggtcgcg gcgggcctgg cccgtccggt cgtctcgccc gccttgatgg ccgcagcgcg
     6541 catgccggcc ggcatgcgcg ccgaacaaga caagcccctt accaccgtgc cgatgccgga
     6601 aatggccacc gcgccagaac cagacaattc gctattgggc agcatcgcgc ttgatcagcc
     6661 cttgcgcgac gcgcgcgacg aattcgagcg catctacttt gaataccatc ttgggcgcga
     6721 gaatcacagc atgacgcgcg tgtccgaaaa aaccggactc gagcgcaccc acctgtaccg
     6781 caagctcaag caactgggca ttgactcgtc gcgcaagaaa aacaattcct gaccgacacg
     6841 ccgccggctc gtgaatgctt ctcgttggac ggctcaggaa tcggtctcgt cgcgtgggcg
     6901 cacgcgcggg cggtgccctg acgcgtccac cggggcggcg gggcggccaa tgactttccg
     6961 cggcatggat cgccatgcat ggccgtaggc gatttcgagc tcgaggtgga tcgtgccgtc
     7021 catatgacgc tgtttttcca gcgccgccgt cagccgctgc ttccaggcgc gccccgccag
     7081 gccccgttgc ctggccaggc tggggttgcc acccaggtcg cggatgtcgt ccagcagctt
     7141 tccggcttcg cggtatgtca gggtgagaat ctcctggtcc atgaccgggt cggagaatcc
     7201 gcgctggatc agcaggtcgc cgaaatcatg catgtcgacg aaggcgggtg tcgccgtgtg
     7261 aatgtcggcg gccgtcagcg cctcgcgcag gttcttcaac gaacccggac ccaggcagga
     7321 aaacatgacc agcgcgcccg gcttgagtgt gcgccgccat tcgctcagca cgtcgtgcgg
     7381 gcgtgggtgc cagtgcagcg ccatgttgga ccagatcagg tcgagtgact cgtcgggcag
     7441 accggacgcg gccatgtcgg cttcgacgaa ccggacggcg cgcgcgggtt tgttgcgcag
     7501 cttgttccac aggcccggct tttccgtata gcgctggcgc gccacgtcga gcagcgcggc
     7561 gcaggagtcc agccctgtgt agtccatctc cgggtagcgc gatctcagcg gctcgatggc
     7621 atggcccgcg ccgcagccgg catccaacac cgacttgggg gccacgcgga tgtagctcag
     7681 gcgctgcagc atgcgttggg cgacttcccc gtacaggaac tgtgccgcat ccagcgggct
     7741 gcggcgcgag aattgctgga tgacgtgggc ggggctcagg ggcagagtcg ggggctgtga
     7801 cattctggtg tgtatcgtct ggttcgcggc gggcggttgt gtttagatgc ccggaggagt
     7861 gtggcgaaga aacggaggct cattatggcg tatcccgagg gcgtgcgcga atcggtgtcg
     7921 gccaccctgg cacggttggc gcgatgcgcc ggtgtttcgc gtcgctgggt cgagagcaag
     7981 gtcgatgccc tgccgtcgga ttgtcttttg tgtcggggcc gggcccgggc cggccggctt
     8041 tgcccagact gtttggccga ggtctcggcc acgatgcggg atggccggcg gcgctgcgcg
     8101 atctgcgcat tgtctctcga agggctggac acctgtcccg actgcgccgc catgccgccg
     8161 tcgtttgata gagtgttggc ggcgttcgac tatgagttcc ctggcgattt gctcattcat
     8221 caactgaaag agacccggcg tttcacgtgt gcgagggtgc tgtctggaat cctggcgcaa
     8281 cgcatccacg tggacccggc cgggctgccg catcatacaa tcctggttcc agtgccggcc
     8341 agccgcgcgt cggtccgcaa gcgtggcttc aaccccgccg cagaagtcgc ccggtcgttg
     8401 gcgtggcggc tggggttggc ttgtcgtccc gccctgctga gacgcgtgcg tgaaggtgtg
     8461 cgccaggcca gtctgtcgcg aggcgagcgc atccggaacg cccggggtct gtacgagtgc
     8521 acggggcgcg tcgatggata cgacatcgcc gtcgtcgatg atgttctgac caccggcagc
     8581 actgcacatg gtatcgcgcg ggcgttcaag gattctggcg ccgcatcggt gagtgtgctg
     8641 gttctcgcac gcacgcgaca tcacttttct tcctgatgga tgactcatgt ttcatatcgt
     8701 actggttgcg cccgaaattc cccccaacac aggcaacgtc atccggttgg cggccaatac
     8761 cggtgcgcgt ctgcatttgg tgcgcccgtt gggtttcgag ctggatgacc ggaaactcaa
     8821 gcgggcgggg ctcgactatc acgagtgggc cgacatgcgg gtgcatgaca cgctggagaa
     8881 agcgttttcc cacattggcg gcgacccggc ccgccgtttc gccatcacga cccgcggcgc
     8941 gcgccatatc gacgcgctca cgttccaggc cggcgatgtc ttcgtgttcg gccgcgagac
     9001 cgccggcttg tcggctgaac aaatggcttg gttccccgac gggcagcgca tccgcctgcc
     9061 catgatgccc gggcagcgca gcctcaacct gtcgaatgcc gtggcggtca cggtgtatga
     9121 ggcttggcgc caagagggct atcggggcgc ggtcggcatc gacgcctagc gtcctgtttg
     9181 actaattcgg ctgcttatcc tcggcgcgtt gcgcagagaa tggcgcccct aatcccgaga
     9241 caggtgcagg cgatcgatca ggccacccca tttcacggta tcggacttga tcagcgcggt
     9301 cagttgctcc ggtgtcgaag gggctggtga aaaatagagg gcttccattc tcttcttcac
     9361 gtcgggccgc cgcagtatct gggtgatcgc cgtgttcagc cgggcaacga tgtccttggg
     9421 tgtgcccgcc ggcacgaaca tggcgtccca cgagatcgac tcgaaccccg gatagccttg
     9481 tgaagccatg gtggggatgc cgggcagtat gtcgctgggt ttgaggctgg tgatggccag
     9541 ggcccgtacc ttgtgcgcct ggatctgggg catggcgatg cccggcacca tgaagctcgc
     9601 ctggatgtca cccgagacga ccgatcgaac gacttccggg aatccggcgt acggaatctg
     9661 ttgcagcttg atgtcggcct tgtgctccag catggccatg cacagatgcg acgagctccc
     9721 ggggccgacg gtcccgaagt tcaatgagtc gggttttgcc ttggccagcg cgacgaattc
     9781 cgacacggtc ttggcggggg agttctgcgg cacgatgagc acgttgggac tggtgccgat
     9841 caacgtgatg ggagtgagtt gatcgggttt gtagcccagt gttttcttgt agagcttggg
     9901 tgcggtgacc atcgggccat tgatggtcat caggatggtg tacccatcag gtttggcgcg
     9961 tgtggcttcg cgtgtgccga tgttgccgcc ggcgccgggt ttgttttcga cgatgacgtg
    10021 ctggcccagc gtcttgccga gtgggtcggc aatgacgcgt gcgagaatgt ccggggacga
    10081 ccctggggga aacggtacga tcatgtgcag gggtttgtcg ggccaggtgc cggcgaacga
    10141 cggtgcgctg aaagtggccg cggccagagc caggcccagg gcggtcttgt gccagatgcg
    10201 gcgggaacgg gaaaatgctt tcatgagcga tccttggcaa gagggaaaat ggaagagcgt
    10261 gtcacggcgg tgaggcggct tcggtgcgtg cctggcgcgc cagcagcccc gcgaccgcct
    10321 gatggggcgc catgccgtcg aacagcactc ggcagaccgc gtgggtgatg gggagatcga
    10381 tatgatgcgc acgtcccagc gccaacgcgg cgcccgcgca gcgcgccccc tcgatggtgg
    10441 tgccgctggc cagcacctgg cgcggatcct ggccctgccc gatggccagt ccggcttgac
    10501 gattccgcga tagcgggccg gtggccgtca ggaccagatc acccaggccg gtcaggccca
    10561 tgaaggtgtc cgcctggcca cccagcgcga cgcccaggcg ttgcatctcg gccaggccgc
    10621 gggtgatcag ggccgcccgg gcattggtgc ccaggcccag tccgtcggcg atgccgcagg
    10681 caatggccat gatgttcttg aggg
//
LOCUS       DASTNK010000089         3815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_247768, whole genome shotgun sequence.
ACCESSION   DASTNK010000089 DASTNK010000000
VERSION     DASTNK010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247768"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VFR20_06855"
     CDS             <1..708
                     /locus_tag="VFR20_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEU0230272.1"
                     /translation="ADPQTNVNLANRLDETPLMYLAIVGQTARARTLIQRGAKVNRLG
                     WTPLHYAASTGQLETAKMLLADKAIVNAPGPDGTTPLMMAALSGSDAMVRLLLHAGAD
                     PTMRNLKGQSAADWARQKGFNDLAAKLDSQAGKVLARRRIQREKDIANQAQTIPVPEL
                     GGAGTPKASSEAYPTGQAADTRNPDSSTQGIVTGVTTPATAPAMPSTKPEKPANPQGD
                     GTSRYFNLGGPVPNAGH"
     gene            complement(728..1549)
                     /locus_tag="VFR20_06860"
     CDS             complement(728..1549)
                     /locus_tag="VFR20_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HEU0230273.1"
                     /translation="MAFPRSATLVRTMRPCLLSLACGLLAACASGPSGNISLIPAGKI
                     AAQTEQNGLVTQSLKWEHHKPGCQGECPTIKLDSIAFPSVPILTKLVDHALATMTGVG
                     GPGPAPYDTIAGYESYFWQTASPRDSTDLSAKARYRNQHLTVIELDTWQYYTGAPHGV
                     SATQFLNWDNARRRVLGIANVLEPGQHPAYVAALDKAYEAWLAHNPEALRDPATYRRI
                     WPFQPSDNFALTDEGMVVKYDSYQIAPYSAGQPELLIPYASLKGILRPQYLPGPG"
     gene            1754..2098
                     /gene="arsC"
                     /locus_tag="VFR20_06865"
     CDS             1754..2098
                     /gene="arsC"
                     /locus_tag="VFR20_06865"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454095.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEU0230274.1"
                     /translation="MTTIYHNPRCGTSRTVLQKLQDNGVNPTIVEYLKTPLDEKALTT
                     LIRHAGLTAREAIRSKEPVFDELGLGGPELSDEQLIAAMAAHPILLNRPFVVTPKGTR
                     LCRPADVLDEIL"
     gene            2141..3013
                     /locus_tag="VFR20_06870"
     CDS             2141..3013
                     /locus_tag="VFR20_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0230275.1"
                     /translation="MPLDLFLLVCLAAVLHATWNLVSKRASSAGASFVLAYRLFSTLL
                     FAPWVIYVLWFDGMAWSLPVVAFIALSSVLHLFYGIYLQRGYQAADLSVVYPIARGTG
                     PLLATAGAFLLLGEQATASGLIGIACVVIGVLLIATQARWRMFLKPQAWVGVRWGVVI
                     GIFIACYTLSDAYSVKALGIVPVVLDWMGGLGMTCMLAPTAWLQRNKLRDRMRGKWGL
                     AFIVGLLSPTAYILVLYALQHGAPVSLVAPLRETSLMVATLASYFILKEKVGPARWLG
                     CIVILLGVVALSGH"
     gene            3131..3568
                     /locus_tag="VFR20_06875"
     CDS             3131..3568
                     /locus_tag="VFR20_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre-like helix-turn-helix
                     domain-containing protein"
                     /protein_id="HEU0230276.1"
                     /translation="MDLAYAYPSSRFEPAPVFDLNSRQARERLSRSALKGFFRLAQAW
                     NLRDEDACALLGGLSSSAFYEWKKKPDRVLEVDRITRISYLVGIYKALHILYGDELAD
                     KWVGLPNRNAIFHGKTPLDHMLDGGLPAMQTVRMLLDARRGGL"
     gene            3572..>3815
                     /locus_tag="VFR20_06880"
     CDS             3572..>3815
                     /locus_tag="VFR20_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HEU0230277.1"
                     /translation="MRHPPLVPLRRADTVRLLPSRFADQEASVLAPLSEDTAHLNDLF
                     DLDNATNQRMAAERGAALGIGVDELVFGVPNFRIINA"
BASE COUNT          695 a         1140 c         1195 g          785 t
ORIGIN      
        1 gccgacccgc agaccaatgt gaaccttgcc aatcgccttg atgaaacgcc gctgatgtat
       61 ctggcgatcg tggggcagac cgcccgcgca cggacgctga tccagcgtgg cgccaaagtc
      121 aaccgtctgg gttggacccc gttgcattac gcagcctcca cggggcagtt ggagaccgcg
      181 aagatgctgc ttgccgacaa ggccatcgtc aacgccccgg ggcccgatgg caccacgcca
      241 ctcatgatgg cggccttgtc gggcagcgac gccatggtcc ggttgttgtt gcacgctggc
      301 gccgacccga ccatgcgcaa cctgaaaggg caaagcgccg ccgattgggc ccgccagaag
      361 ggtttcaatg acctggccgc caaactggac tctcaggctg ggaaagtgct ggcccggcgt
      421 cgcatacagc gcgaaaaaga catcgccaat caggctcaga ccataccggt gcccgaactg
      481 ggtggcgctg gcactcccaa ggcatcatcc gaagcgtacc ccaccggcca ggcggcggac
      541 acacggaacc cggactcctc gacgcagggt atcgtgaccg gcgtcacaac accggccacg
      601 gcgcccgcca tgccatcgac caagcctgaa aagcccgcga atccccaggg tgacggcaca
      661 tcacggtact tcaaccttgg gggacccgtg cccaacgcgg ggcattgagc attcaagtcg
      721 agagtgttca gccggggccc ggcaggtact gggggcgcag aatgcctttg agcgacgcgt
      781 agggaatgag cagttccggc tggcccgccg agtacggcgc gatctggtac gaatcgtact
      841 tcaccaccat tccctcgtcc gtcaatgcga aattgtcgct gggttggaac ggccagatgc
      901 ggcgataggt cgcggggtcg cgtagcgcct ccgggttgtg cgccagccac gcttcgtaag
      961 ccttgtccaa cgccgccaca taggcggggt gttggcctgg ttccaggaca ttggcgatgc
     1021 ccaggactcg ccggcgcgca ttgtcccaat tcaggaattg ggtcgccgac acgccatggg
     1081 gggcgccggt gtaatactgc caagtgtcga gttcgatcac cgtgaggtgc tggttgcggt
     1141 agcgtgcctt ggccgacagg tcggtggaat cacggggaga cgcggtctgc cagaaatacg
     1201 actcgtaacc tgcgatcgtg tcataggggg cggggcccgg accacccacc ccggtcatgg
     1261 tggccagggc gtggtcgacg agtttggtga gaatcggcac gctcgggaac gcgatggagt
     1321 ccagtttgat ggtggggcat tcgccctgac agcctggttt gtggtgttcc catttgagcg
     1381 attgtgtgac cagaccgttt tgttcggttt gcgccgcgat cttgcccgcc ggaatgaggg
     1441 atatattgcc cgacgggcca ctagcgcatg cggccaacag cccgcacgcc agcgacaaca
     1501 gacatggacg catcgtgcgg acgagggtgg cggatcgcgg aaatgccatg aagattccat
     1561 tggaggcttg atcggcctca ctttaccagt ggccgggcgg ggccggcaaa caggcggcat
     1621 ccaacctgcc tgtgcctgat ccgtgcgcgg tccgcttatg gctggtgatg gtcaggggtc
     1681 cattggcttt tgaccttatg cctttcacag taacatagcg atatctcaag catagtgcgc
     1741 aaagggaaga gcaatgacca cgatttacca taatccgcgc tgcggcacgt cgcgcacggt
     1801 gttgcagaag ttgcaggata acggtgtcaa cccaaccatc gtggaatatc tcaagacccc
     1861 tctggatgag aaggcgctca caacgctcat ccgccatgcc gggttgaccg ctcgtgaggc
     1921 tatccggtcc aaagagccgg ttttcgatga gctggggttg ggtgggccgg aactgtccga
     1981 tgaacaattg atcgccgcca tggctgcgca ccccatcttg ttgaaccgcc cattcgtggt
     2041 cacacccaaa ggcacgcgcc tgtgccggcc ggcggacgtc ctggacgaga tcctctgatc
     2101 aacaggccga ccccgccgcc gctaacaaaa cctccactcc gtgccgcttg atctgtttct
     2161 gctggtttgc ctcgccgcgg tcctgcacgc cacctggaac ctggtttcca agcgggcatc
     2221 gtcggcgggt gcctcattcg tattggcata tcgcctgttt tccacgctgc tgttcgcgcc
     2281 gtgggtgatc tatgtgcttt ggttcgacgg tatggcgtgg agtctgcctg tcgtggcgtt
     2341 cattgcgttg tccagtgtgt tgcacctgtt ttatggcatc tatctacaga gaggttacca
     2401 agcggccgac ctgtctgtgg tgtaccccat tgcgcgtggc acggggccgt tgctggccac
     2461 ggctggcgcg ttcctgttgc tgggcgaaca agccaccgcc tcggggttga tcgggatcgc
     2521 ttgtgtggtg attggcgtat tgctcattgc cactcaggcg cgctggcgca tgttcctgaa
     2581 gccgcaggca tgggtagggg tgcgctgggg agtggtcatc gggattttca tcgcttgcta
     2641 cacgctgtcc gatgcttata gtgtcaaagc actcggcatc gttccggttg ttctggactg
     2701 gatgggcggc cttggcatga cctgtatgtt ggcgcccacg gcatggctcc aacggaacaa
     2761 gctgcgcgac aggatgcggg gtaaatgggg gctcgccttc atcgttggac tcttgtcgcc
     2821 aacagcctac atactagtgt tgtatgcgct ccagcatggc gcgccggtga gtctcgtcgc
     2881 gccgttgcgg gaaacctcgt tgatggttgc cacgttggcc agttatttca ttctcaaaga
     2941 aaaggtcggc ccggcgcgtt ggctgggatg tatcgtgatt ctactgggtg tggtggccct
     3001 gtccggtcat tgaccggttc atgccgccga atttgatggc acgactccca tgtctgggca
     3061 gtcatcagca tggctgtctg gagttatcca aaaaaagaga ataaactctc catatacgga
     3121 gggtcacatc gtggatctcg cttacgccta tcccagcagt cgtttcgaac ccgcacccgt
     3181 gttcgatctg aacagtcggc aggcccgtga acgtctgtcc cggtcggccc taaaaggatt
     3241 cttccgactg gcccaggcct ggaacctgcg cgatgaagac gcttgcgcgc tgttgggcgg
     3301 gttgtccagc agcgcgtttt acgaatggaa aaagaaaccc gaccgcgtgc tcgaggtcga
     3361 tcgcatcacg cgtatttcgt acctggtcgg catctacaag gcgctgcaca tcctctacgg
     3421 cgatgagctg gccgataaat gggtcggtct gcccaaccgc aacgccatat tccacggcaa
     3481 aacgccactc gatcacatgc tggatggcgg gttgccggcc atgcaaaccg tgcgcatgct
     3541 gctcgatgcg cggcgcggcg ggctgtaatc cttgcgccat ccccccctgg tccctctgcg
     3601 ccgcgccgac acggtgcggt tgctgccctc gcgcttcgct gatcaggagg cgtcggttct
     3661 ggctccgctt tcagaagaca ccgcgcacct caatgatttg ttcgacctgg acaacgccac
     3721 caaccagcgt atggcggccg agcgtggcgc ggcgctgggc ataggcgtgg atgaactcgt
     3781 gttcggcgtc cccaacttcc gcatcatcaa tgcgg
//
LOCUS       DASTNK010000090        27266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1360349, whole genome shotgun sequence.
ACCESSION   DASTNK010000090 DASTNK010000000
VERSION     DASTNK010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 27266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27266
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360349"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFR20_06885"
     CDS             complement(<1..328)
                     /locus_tag="VFR20_06885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU0230278.1"
                     /translation="MAILRLRGLLGRGARMRRANLHAQLMAIHRVQAVVEFDLQGHIL
                     MANQNFLDTVGYSLPEIVGKHHLLFVDAQERTSPAYVAFWQKLGQGLHDAGCYKRIAK
                     DGREVWL"
     gene            506..718
                     /locus_tag="VFR20_06890"
     CDS             506..718
                     /locus_tag="VFR20_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230279.1"
                     /translation="MNTWYILPNGNIRHIDGLELQPEKDWFPTEASMAAFTDAQRAAG
                     KSDAEIIRMMMGLAIDCEAWVGENLS"
     gene            922..1404
                     /locus_tag="VFR20_06895"
     CDS             922..1404
                     /locus_tag="VFR20_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEU0230280.1"
                     /translation="MPSFDVVSEVDAHELTNAVDQARRELATRFDFKGADAKFELEGF
                     VVTQFAPSAFQANQMLDILRGRLVARGIDVRCMDVAAPSENLAGVRQKVTIKQGIEQA
                     VSKKLIAAIKGAKLKVETQINGDKLRVTSKKRDDLQTAIALLRKTDVELPLQFNNFRD
                     "
     gene            1578..1814
                     /locus_tag="VFR20_06900"
     CDS             1578..1814
                     /locus_tag="VFR20_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU0230281.1"
                     /translation="MQVADNLEAEILAIVESVILKPVAADASLVKSGLVDSMAAVDIT
                     LAIEDKYGCAIPAPEIAEIMETVQSIRGYVAAHT"
     gene            1795..2979
                     /locus_tag="VFR20_06905"
     CDS             1795..2979
                     /locus_tag="VFR20_06905"
                     /inference="COORDINATES: protein motif:HMM:NF016782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEU0230282.1"
                     /translation="MWRRILDTGLSRHLLAALTAVGIALLAYFGLNPYLTRAAHPRAT
                     DGLKIQAGVYLPNMGDNWNEQEVNFARMRQALADGTLVVMGSSELSSHDLRFVPYRFF
                     PAELGVPTLAYGHAKLQTYGIFRVLQSLSAGFTPKTKLVIMLSPAWFADEGDMPSSAF
                     AEHISGPVLDSLWGQPAAREGLIHWARTHETLGILGLLAQAKLAAFRDKLQMWLTGRP
                     IASGPAYVQAPAALAATRDASFPVPGALPQADWPRLLSDARLKDQKLCGANRYLVCDH
                     YYQKHISRVPDPAHQEFAHGNPLMAPEYADLSRLMAYLAEKKVHALFIMQPLNTKLVL
                     DTKKFVPTVRSVMAMCRRYHMSCVNMYPLHQAPGIFKDDMHLAALGWALADEAIDRHF
                     AQ"
     gene            2976..3101
                     /locus_tag="VFR20_06910"
     CDS             2976..3101
                     /locus_tag="VFR20_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230283.1"
                     /translation="MKRYLLLVCLYLGLIAVCLLQARAPSDLGGPIKVEYQYQQF"
     gene            3122..4249
                     /locus_tag="VFR20_06915"
     CDS             3122..4249
                     /locus_tag="VFR20_06915"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEU0230284.1"
                     /translation="MELFASFDFFQYALLAGLGVSLTSRAVAWTRLPLRYVLACLGLL
                     TAALVFQNRPLQPLIVFAVQAVALCLCRRKIIPTRAAVVLTMLPLLLVKTHAVGMLGM
                     LGLSFMTFRAVDVLLFAHDNERIHPLDYVTYLFFPLTLLAGPMYRWRNFQTDLNHGYA
                     QVDMSHWREGFEYLLLGLTQKFAIAHLVWIAGLAQVAPNDYSLRGVVLNTVLYSVYLY
                     FDFAGYSNMAVGLGRMFGVNLPANFNNPVLARNPQDFWRRWHISLSEWLRDVVFMPIY
                     KALSRNTYFSRHRFAAQNIGIFATLFCMGIWNGLQWHYIVSGIMFGTYSVIHNRLLLA
                     AREHPPLQRWLSTRTARLLGRFLTLASAMLALYVFSGRSPV"
     gene            4344..5795
                     /locus_tag="VFR20_06920"
     CDS             4344..5795
                     /locus_tag="VFR20_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HEU0230285.1"
                     /translation="MHLDLRTMRFADTDLDQDRQAVVGCDRSLSWQTLKEEAQRWLGR
                     AIELGARPDVPIVLSGHKQAAFFTAMTACLILGAPFVPVDSIYPDERRQRIAELVGAA
                     LHYRADTDTFEPTGQAASPLEEKGLAYVLFTSGSTGEPKGVQIGRESVQLLSQWMRTR
                     FGLGEAPVFMNQAPFSFDLSMFEVFGTLGSGGTCVLNARELIQDGPAFLQRLARERIT
                     CWVSTPSFAHQQLLNKTFSGTDLPTVNTFLFCGEVLPHALCVRLRQRFPQARILNTYG
                     PTEATVATTLIEVDQAVLDAHKVLPVGYAKPECLIEIHDGEICVVGDHVMRGYLNQPD
                     LNQARMFRHTDGRRGLRTGDLGHLDADGLLWCQGRRDDQVKLNGYRIELPEIALALQS
                     LDEVKNAVCLALRRPDNSVARLVGFVELTEHGRPSGAIEWAPYPATNWKPPLERRLPP
                     YMLPSEIMVVDAFPMSVNQKIDGKALLETYLGK"
     gene            complement(5890..6960)
                     /locus_tag="VFR20_06925"
     CDS             complement(5890..6960)
                     /locus_tag="VFR20_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEU0230286.1"
                     /translation="MHNAPIKGRGSLSNDVSRFDMLQRETFDDGWVNENRPEESRPAT
                     TVTHETARSIISRNDSPDLYFDQSINPYRGCEHGCVYCYARPSHAYLGHSPGLDFETR
                     LYAKHNAADVLRKTLSNPSYVPRLIALGANTDPYQPVERRLRITASILDVLDEFNHPV
                     AITTKSASVTQDLDLLARMAARNLVCVHVSVGTLDRHIARTLEPRASTPGARLDAVRT
                     LSDAGIPTGVIVAPVIPSLNDHDLENIIFRAKAEGARSAHYVLLRLPMEIAGLFQEWL
                     AAHHPLKQAHVMNLVRQTRGGKVYDANFQTRMRGAGVVAGLIEQRFRKACQKAGLTQR
                     RVALDVSHFRRPTRGSAQLALF"
     gene            7102..7323
                     /locus_tag="VFR20_06930"
     CDS             7102..7323
                     /locus_tag="VFR20_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007737589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HEU0230287.1"
                     /translation="MTIGSTLRAVALAGACASMLSACVVEPVRPPRPAPVMEIIPPPP
                     AAGYHWVTGHYRWRRNHWKWVPGHWKYMR"
     gene            7422..8027
                     /locus_tag="VFR20_06935"
     CDS             7422..8027
                     /locus_tag="VFR20_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006383920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEU0230288.1"
                     /translation="MRALLYVLPLLLLAGCATTGAPPSEPNNLCAIFRQRPAWYQAAI
                     HAQEKWGVPPQVPMAIMYQESSFRAKALPPRYYALGFIPWGRVSSAYGYAQAKDDTWA
                     DYQRDAGSWFSDRDDFDDAMDFMGWYISKTQKIDGVSKWDAYNQYLAYHEGWTGYRQR
                     SYTRKPWLMRVARRVQERAERFGAQYRGCEKQLSAADGSFG"
     gene            complement(8116..9033)
                     /gene="epsC"
                     /locus_tag="VFR20_06940"
     CDS             complement(8116..9033)
                     /gene="epsC"
                     /locus_tag="VFR20_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HEU0230289.1"
                     /translation="MAVFDVGDIVQELDSIRGAWRDSQRRAREPHAREFPSRDVLAHI
                     VDQLKGALFPMRLGPENLRHDGENLYVGYTLNCALQSLFTQAKLELRYQARHEAADAS
                     GIEQRALEIVNRFSESLPGIRRLLDQDVLAAYQGDPAAGSVDEVLLCYPGILALIHYR
                     LAHQLYLSGLHLLARIITEIAHSETGIDIHPGAQIGASCFIDHGTGVVIGETARLGTG
                     VRIYQAVTLGAKRFPADADGSLKKGLPRHPIVEDDVVIYAGATILGRVTLGKGAVIGG
                     NVWVTEDVEPGAALSQASSQSAPRQRQGV"
     gene            complement(9293..10513)
                     /locus_tag="VFR20_06945"
     CDS             complement(9293..10513)
                     /locus_tag="VFR20_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0230290.1"
                     /translation="MHHSPSRRTPVAHPPGLVLFALAIGAFAIGTTEFATMSLLPYFA
                     VDLKIDAPTAGHVISAYALGVVVGAPVLTVLAARMARRTLLLWLMAMFSLFNGLCAFA
                     PSYHWLLVLRFLSGLPHGAYFGIGALVAVSLVPLNKRSQAVGRMFLGLTAATIIGVPF
                     ANWLGHTVSWRWGFGLVAVTGVLTVIMVGWTVPRSPAEPGSSPLRELGALRQPQVWLT
                     LATGAIGFGGLFAVYTYLADTLMHVTGVSAGIIPLVLGVFGVGMTVGNIVVPWFADRA
                     LMPTAGGLLVWSALATAFFPLAAGNAWTVSVDVFLIGVGGALGTILQTRLMDVARDAQ
                     GLAAALNHSAFNFANALGPFLGGIAIAHGLGWASTGLVGSGLALGGLVLLLVSVWFGR
                     ARIAGSPAAPVYKA"
     gene            10782..11684
                     /locus_tag="VFR20_06950"
     CDS             10782..11684
                     /locus_tag="VFR20_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEU0230291.1"
                     /translation="MIKWGLIALWIASITYVYFRGRIRVSFLRTVLDHSAVLAPVNVF
                     MYLFSAVPARPFIPVEHLAGLRALDDNWETIRDEAKRLHDAQQIRAAEKNNDAGFNSF
                     FKTGWKRFYLKWYEAEHPSAAQLCPQTVRILHDIPQVKAAMFAVLPPGGKLNPHRDPF
                     AGSLRYHLGLATPNDDRCFIEVDGERYSWRDGKSVVFDETYIHWAENTSDQTRIILFC
                     DLERPLRFSWASRFNRWFSRIFMTLASSPNQDGDQTGFINRLFFIAWKIGQYRRRLKR
                     WNRTVYQGVRVALAIAILALIVYI"
     gene            12041..13042
                     /locus_tag="VFR20_06955"
     CDS             12041..13042
                     /locus_tag="VFR20_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEU0230292.1"
                     /translation="MNLSSSRLWTAMGFTVALSFAGQALADSPIVIKFSHVVADATPK
                     GQGALKFKEVAEKLLPGKVQVQVFPNSQLFGDAKEMEAVLLGDVQFIAPSLSKFDRYT
                     KKVQLFDLPFLFDDIAAVDRFQHSDAGKALLNSMQNRGLQGLAYWHNGLKQLSTNKEK
                     LTRPEDVAGLKFRIQNSDVLEAQFRQLKANPQKLAFSEVYQALQTGVVDGQENTWSNI
                     YSQKFHEVQKTIANTNHGVIDYMVVTNAKWWQGLPEDVRAGLTQAMEEATVYANTRAA
                     ELNDRDRERIAQAGGAKIQALSKEDKAAWRQAMAPVWQKFESQIGTDLIKAAQESNT"
     gene            13110..13664
                     /locus_tag="VFR20_06960"
     CDS             13110..13664
                     /locus_tag="VFR20_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEU0230293.1"
                     /translation="MKSAWFGRLEEGLIALLLAAMTLITFGQVVARYLFNYSFVWALE
                     LSTYLFGGLIFLGMSYGVRVNAHIGVDALVKLLPKPTAHRLGIVAVLLCLVYAGIVFA
                     GGWVYVAKIQQIGILAQDIPIPQWIPRVVLPLGFALLFFRFTEILVRMLRGQETHLLG
                     DEADDALKLRADANAPATPEGKKA"
     gene            13661..14941
                     /locus_tag="VFR20_06965"
     CDS             13661..14941
                     /locus_tag="VFR20_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018025729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEU0230294.1"
                     /translation="MTTTALFLMLFVFMFMGLPVAVALGLSSILTILTFGHDSLASLS
                     LKVFETSEHFTLMAIPFFILAGAFMTTGGVAKRMIRFATACVGHLHGGLAIASVMACV
                     LFAAVSGSSPATVVAVGSIVIAGMVRAGYPQSFAAGVICNAGTLGILVPPSIVMVVYG
                     AATETSVGRLFMAGVLPGALLGLMLMTVIYLIARVRKLPRQPRASLGEVFGAARESFW
                     GLMLIVIILGGIYGGIFTPTEAAAVAAVYAFAVAVFVYRDIGMRQVPGILIDAAKVTV
                     MLMFIIANALLFAHVLTTERIPQMIAEHIVAWNMSAWQFLIVVNILLLIAGAFMEPTG
                     IILILAPILFPIATKLGIDPVHLGIIIVVNLEIGMVTPPIGLNLFVTAGITNMNMGQV
                     IRAAVPWLSVLLVFLAFITYVPQVSLWLPNMGFK"
     gene            15090..15434
                     /locus_tag="VFR20_06970"
     CDS             15090..15434
                     /locus_tag="VFR20_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEU0230295.1"
                     /translation="MNLRRHLTQIAVSSVVTLAVCTTAVASERRTANTVLGAGLGAVA
                     GAVLSDGDPLLTLGGAAAGGLLGNVLTEDRRHYHGRYDGAHRRRVEERYGYHHERRRG
                     YDRGWRRSHRGH"
     gene            complement(15428..15859)
                     /locus_tag="VFR20_06975"
     CDS             complement(15428..15859)
                     /locus_tag="VFR20_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0230296.1"
                     /translation="MEIHPYLIFEGRCEEALAFYEESLGAEVVQLLRFKDNPEANGAC
                     SGSPENQEKIMHVSFRIGDAMLMASDGMCSGEPKFQGVSIVLTAPDGAAAERMFAALG
                     KDGQVTMPMDRTFFASHFGMVADRFGVHWMLIVPNPVGGNQ"
     gene            16010..16675
                     /locus_tag="VFR20_06980"
     CDS             16010..16675
                     /locus_tag="VFR20_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0230297.1"
                     /translation="MDSPPDVSARTLKRPKERGTYRHGDLKRALLEAGMELARQGGPE
                     AVVLREATRRVGVVPNAAYRHFTNRDALLQAVRAAALSALAQAMENELARIPAELPSS
                     ARARASVRAIGTGYLTFAHEEPGLFRTAFAMPDSVEDDLDPAKTGRSGLNPFQLLGKA
                     LDELVTAKTLPATRRPGAEYLAWSAVHGLAMLMLEGPLRSLAPAQAHAVGEHLLDMIE
                     RGL"
     gene            16863..17351
                     /locus_tag="VFR20_06985"
     CDS             16863..17351
                     /locus_tag="VFR20_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013951861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0230298.1"
                     /translation="MSFESRISRFCKTHPNAPREHILASRLLFRTARLLHERIDLALA
                     PFSLNMSQYLVLSMLAADEGEPSSPSEVGSTLDATRTQMTRALDALQAQGLIRRRASA
                     QDRRSLELELTEAGRQMVENAAPAVHAVYAQAWSAVKPADRTVTTSSLQRLHTGLEST
                     QP"
     gene            17348..18925
                     /locus_tag="VFR20_06990"
     CDS             17348..18925
                     /locus_tag="VFR20_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006164641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0230299.1"
                     /translation="MNHPLRRKMRPRRLLGLARLLSHRERQLISMMLLALIVSVEFFE
                     NVMFLFSASHVMGGIGADPRGFALAMSTYAVGSLLMIVNQQWLAQRFGYRRYLTVALT
                     LFTIGTLMAASSTDLTQLVIARFVQGFGGGALFTSSRVLIPIMFSPADRPRAQRIFMI
                     GIFGATAMAPAIAAELMDHGVWQDVFYGAVPLSVIAAVGAWRLLPDAEPRERTHQPLL
                     VPLLWFGLAVAALQAALTEARFDMFSHPVRPALIAIAGALLLIGFLHHQWHHHQPLLQ
                     LHALRNPVYLTGLSLYFMYYLISYFNSYLFPIYAEHALGLPLSTVGWLDTSAGLMSFV
                     GILAYIRYAKLIKSKKPLMVAGLLIMACAAWRFSSMPPDVTPWDLIPSLAMKGLFAVL
                     FVIPVAGLTYKNLTDNTFAHGYQSKNLMRQVSGSLAPALGAILLQNRQFSVHTSLVDS
                     TGNQPGITMGWLNQVQTALIAHGMDAGSAARVAMAQLTGMLDRQAMLIASEDLYRLIM
                     VVAIAGACIVMAQRRLA"
     gene            19046..21898
                     /locus_tag="VFR20_06995"
     CDS             19046..21898
                     /locus_tag="VFR20_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HEU0230300.1"
                     /translation="MPLTLILALPFAGSVLAATLPSNARNQAAWLAGLIALVCAVLVA
                     VQFPIVAHGGVAQTTLAWLPSLGLNLVLRMDGYAWLFALLVAGMGALIVLYARYYMSP
                     DDPVPRFFSFFLAFMGAMLGVVLSGNIIQLAIFWELTSLSSFMLIAYWHHRSDARRGA
                     RMALTITVAGGLCLLAGMLLLGRIVGSYDLGQVLASANRIRASAWYPTVLTLVALGAL
                     AKSAQFPFHVWLPNAMAAPTPVSAYLHSATMVKAGVFLLARFWPVLSGTDQWFWIIGG
                     AGLCSLVLGAFAAMFQQDMKGILAYSTISHLGLITLLLGMNSRLALVAAIFHMMNHAT
                     FKSSLFMATGIVDHETGTRDLDRLSGLRRTMPVTATLAMVAAAAMAGVPLLNGFISKE
                     MFFAETVFISGHWITRVGLPVAAVVAGAFSVAYSLRFIRQVFFGQPTQDLPHRPHDPP
                     LWMLLPSALLVLACLVVGVLPGLTLGPLLHAAALSILGAEHMPSYDLVVWHGFNLPLA
                     MSMLAMLGGLVLLHLFRGRYRSRPGLTPLLHRLDGRRAFETLLESLDGIAAALFSWTW
                     SPRLQRQVALIVAITIAVALPPLRHGWPAGTLSTSVEPSFIILWIAGTACALGAAATA
                     RRHRLTSLALVGGAGLCTCITFAWLSAPDLALTQLAVETVTTILLLLGLRWLPQRAEH
                     NDAARPMTRDLPGMLLAVAGGAGLATLAYAVMTHPQNPGIASYFIEHALPAGGGGNVV
                     NVILVDFRGFDTLGEITVLGIVALTVYALLRRFRPSPESLAVTATTAMPEAMDVPAVL
                     TRLLLPLAGLISIYFLLRGHNLPGGGFVGGLIFATAIIVQYMTSGIAWVESRPRLQPQ
                     YWIAAGLLIAGITAVSAWWAARPLLTALTWNPVLPLIGEVHISSVLFFDIGVYMLVIG
                     ATMLILIALAHQSLRFHRAPATEPASGTQPAPSGAP"
     gene            21898..22233
                     /locus_tag="VFR20_07000"
     CDS             21898..22233
                     /locus_tag="VFR20_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HEU0230301.1"
                     /translation="MEIVLAIVIGILGGTGIWLLLRPRTFQFIMGLSLLSYAVNLFIF
                     AMGRLAIGAAPVIAPGAAGHTVYDDPVPQALVLTAIVIGFATTALFLVVLLAARGFSG
                     TDHVDGQEP"
     gene            22230..23924
                     /locus_tag="VFR20_07005"
     CDS             22230..23924
                     /locus_tag="VFR20_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HEU0230302.1"
                     /translation="MMPAGWSHHLPILPIVIPLAAGAAMLMLKESNRQARVTLALAST
                     LAQITVAVALLCMTAGAIPGFGPHDIGAYLLGDWPAPFGIVVVADRLAAVLLTLNAAM
                     GLCVLVYACARWDRAGVHFHPLFQFLLMGLNGAFLTGDLFNLFVFFEILLAASYGLML
                     HGSGAARVGSGLHYIAVNLVASLLLLIGIALIYGVTGTLNMADLAARAGGLANTDRRF
                     FEAGAAILGMGFLIKAAAWPLNFWLPGAYASASAPVAAVFAIMTKVGVYALLRMGSLL
                     LPTQAPAAFRGTWMFSIGLATMVFGTLGLLTARRIPRLAAYSVIISTGTLLAALGMPG
                     ITLTGPALFYLISSVLAVAALFLLAEMVERTQPFGAGLLTVTQEAFHPDEDETLDQNP
                     DDSGIAIPSAMVFLGLSFAACALLITGLPPLSGFVAKFALMSTLLNMPGTTAPSLEAW
                     LLFTAILVSGMAGVIALGRAGIQTFWSTEDIIVPHLRLGEAAPVAVLVAGCIVLTLRA
                     DPVLIYLKATAASLDHPAYYIEAVLSQRPVVRPPDAGSGAGAGAESGAGAESGAHP"
     gene            23921..24418
                     /locus_tag="VFR20_07010"
     CDS             23921..24418
                     /locus_tag="VFR20_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEU0230303.1"
                     /translation="MIRRCLRRAALPLCLLAAWLLLQGTFALGEALLGLALALALVRA
                     AAPLCPRHAFPRRPRVMLGLLGLVLLDILQSNVMVARLIWSGRRRRPTPGFVRIPLAL
                     RDLNALATLACIITCTPGTVWVDLSPDHVLTLHVLDLDNESAWIQTIQQRYESRLKEI
                     FKCPT"
     gene            24406..24687
                     /locus_tag="VFR20_07015"
     CDS             24406..24687
                     /locus_tag="VFR20_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HEU0230304.1"
                     /translation="MPHLIDWACAFSLGCLALGLACAGWRLLRGPTPPDRVLALDTMY
                     LNGMLMILILGLRIGTDLYFDIALLISLLGFASSMAMAKYLLRGEVIEP"
     gene            24684..25010
                     /gene="mnhG"
                     /locus_tag="VFR20_07020"
     CDS             24684..25010
                     /gene="mnhG"
                     /locus_tag="VFR20_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HEU0230305.1"
                     /translation="MNTLPLWAGIPAAILIVLGGLIALTGSLGLLRFSEFHARIHAPA
                     IINTLGATCLVAASILIWFALDSGPIFQEILILLFLVITSPITAMLLMRAASRHHGDN
                     EQRHVD"
     gene            25149..25568
                     /locus_tag="VFR20_07025"
     CDS             25149..25568
                     /locus_tag="VFR20_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230306.1"
                     /translation="MTAPYFASHKHLVARLFCATLLATAASAAGAYTTHSMNVSPEAQ
                     YQADVAHCKSGQSNEDQATCLKEAGAALEAAKRGKLTTAPSYTTDQTKRCQELPANKQ
                     QDCLALMSDQGSTHVEGSVNGGGVLRETTITVPAGSQ"
     gene            complement(25618..>27266)
                     /locus_tag="VFR20_07030"
     CDS             complement(25618..>27266)
                     /locus_tag="VFR20_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEU0230307.1"
                     /translation="GEGGTLALTELAVRGMTRRRGMVLTVLGLIGAALFYGDSMITPA
                     ISVLSAVEGIGVVSHELDSLIVPISVVVLAALFLIQSRGTGTVGKLFGPIMLLWFITL
                     GVLGAWRIWLTPKILLALNPLYAWRLITDGPLEAFLLLGAVVLALTGAEALYADMGHY
                     GRTAIRRAWFAVVMPGLVLCYFGQGAVLMANPAAISNPFFFMAPSWGLIPLVALATIA
                     TVIASQSVISGAFSVTRQAVQLGFWPLMAIQHTSAREEGQIYMPRVNSLLFVAVVVLV
                     VVFQSSDRLAQAYGFAVTGTMLMTSLLAFAVLPRSSRGPRKYLWWPLLTVFLILDILL
                     FSSNATKLFEGGWLPMLVGISLLTLMLTWKQGREKMHHNLAGDQPPLKSFMEGLEQYP
                     PTRVEGTSVFMSMIYGTVPPALLHNLKHNKVLHEQALFLTVESADVPYVPFDERFELE
                     RLSRSSWQAIARWGFKQEPNVPQVLEQITQAHPEINLEPMQTSYFLSRQTVIVVRRLP
                     LRLRWRRSLFAFMARNATRSTRFYKIPPNRVVEMGMQQEL"
BASE COUNT         5123 a         8968 c         7998 g         5177 t
ORIGIN      
        1 gcagccaaac ctcgcgcccg tctttggcaa tgcgcttgta gcacccggca tcgtgcaaac
       61 cctgcccaag tttttgccag aacgcgacgt aggcgggcga cgttcgctcc tgggcatcga
      121 cgaaaagcaa atgatgtttc ccgacgattt ccggcagaga atacccaacc gtgtcgagaa
      181 aattctggtt ggccatcaga atatggcctt gcagatcgaa ctccacgacg gcctgcaccc
      241 tgtgaatagc catcagttgc gcatgcagat tcgcccgccg catccgcgca ccgcgcccaa
      301 gcaggccgcg caaacgtaaa atcgccattc tcacctcccc caccaagtac aaaggaatgc
      361 ggatagcggg gggagtggac gctcatcccg cgcctttgat ccaaccaccc cttccgcccc
      421 caccattacg gcacttggcg ggatggcttt agcatcattt tcggatcacg gaggaaagcg
      481 gatcaaggcg tggtacaaca ccaccatgaa cacttggtac atactgccca atggcaatat
      541 ccgacacatc gacggactgg agctgcagcc cgagaaagac tggttcccga ccgaggccag
      601 catggccgcc ttcaccgacg cgcaacgcgc ggcggggaaa tccgatgccg aaatcatccg
      661 gatgatgatg gggctggcta tcgactgcga agcgtgggtc ggggagaacc tgagctgatt
      721 ggccaatctc cctgaacaga ctggcctagc ccaccgaaca gatagactgg ccggtttcgt
      781 ttatgacggc catgcccgtc gatgatattc ccctccgcaa tcaggcacca cgccaaacgc
      841 tccggtcact ataattactt ggcgatacca acacaggacg ggtgacgcgc ccgccacaat
      901 ctatccagga gatcatccgc catgccgtct ttcgacgtcg tgtccgaagt ggacgcccac
      961 gaactcacca acgccgtcga ccaggcgcgc cgcgagctgg ccacgcgctt cgacttcaaa
     1021 ggcgcggatg cgaagttcga actcgaaggc ttcgtggtga cacaattcgc acccagcgca
     1081 ttccaggcga accagatgct ggacatcctg cgggggcggc tggtcgcgcg cgggattgat
     1141 gtccgctgca tggacgtcgc ggccccgtcc gagaatctgg ctggggtgcg ccaaaaagtc
     1201 accatcaaac aaggtatcga acaggccgtg tcgaaaaagc tgatcgcggc catcaaaggc
     1261 gcgaaactga aggtcgaaac ccagatcaac ggcgacaagc tgcgcgtgac cagcaaaaag
     1321 cgtgacgatc tgcaaacggc catcgccctg ttgcgcaaga ccgacgttga acttcctctg
     1381 caattcaaca acttccgcga ctgacacccg caatccaagc catggcggca aaacccatgc
     1441 ccaaggcacg ggttttgcgc gcccgcaacg gtcccagaaa ttttaagctt gcattcagct
     1501 tatctggccg ataattcgtc gccgcgtttt tcccatattt ttgcgttcgg ctctggccga
     1561 aagcgtagag ttcccccatg caggtcgcag acaacctgga agcagaaatc ctcgccatcg
     1621 tcgaatcggt cattctcaaa cccgtcgccg ccgacgcatc gctggtgaag tcgggactgg
     1681 tggactccat ggcggccgtc gacatcacac tggcgattga ggacaagtac ggctgtgcca
     1741 ttccggcgcc ggaaatcgcc gaaatcatgg agacggtgca aagcatccgc gggtatgtgg
     1801 cggcgcatac ttgacacagg cttatcccgg cacctgctcg ccgcactgac cgctgttggc
     1861 atcgctctgc tggcctattt cggcctcaat ccctatctga cccgtgccgc gcacccccgg
     1921 gccaccgacg gcctgaagat ccaggcgggg gtctatctgc ccaacatggg cgataactgg
     1981 aacgagcaag aggtcaattt cgcccgcatg cgacaggcgc tggccgatgg caccctggtc
     2041 gtcatggggt cgtcggaatt gtccagccac gaccttcggt tcgtgccgta ccgttttttc
     2101 ccggccgagc tgggcgtgcc caccttggcc tacggacatg ccaagctgca gacgtacggc
     2161 attttccgcg tgctgcagtc cctgtcggcg ggcttcacac ccaaaaccaa gctggtcatc
     2221 atgttgtcgc ccgcctggtt cgccgacgaa ggtgacatgc catccagcgc gttcgcggag
     2281 cacatttcgg gccccgtgct ggatagcctg tggggtcagc cggcagcccg cgaaggcttg
     2341 atccactggg cacgcaccca tgaaaccctc ggcatactcg ggttgctggc gcaggccaag
     2401 ttggcggcat tccgggacaa actgcagatg tggctcaccg gtcgccccat cgccagcggc
     2461 ccggcctatg ttcaagcgcc cgccgccctc gccgccactc gcgacgccag cttccccgtg
     2521 cccggcgccc tgccccaggc cgactggccc cgcctgttga gcgacgcgcg tctcaaggat
     2581 cagaagctgt gtggcgccaa ccggtacctc gtgtgcgatc actattatca aaaacacata
     2641 tcgcgggtgc ccgaccccgc ccaccaggaa ttcgcccacg gcaatcctct gatggccccg
     2701 gagtatgccg acctgtcgcg cctcatggcg tatctggctg aaaagaaagt acacgccttg
     2761 ttcatcatgc aacccttgaa caccaaactg gtgctggata caaagaagtt cgtacccacc
     2821 gttcgcagcg tgatggcgat gtgccgacgc taccacatgt cctgcgtgaa catgtatccg
     2881 ctacatcagg cgcccggcat cttcaaagac gacatgcact tggcggcgct gggatgggca
     2941 ttggccgacg aagccataga ccgacacttc gcacaatgaa acgctacctt ctgcttgtct
     3001 gcctgtatct gggcctgatc gccgtgtgtc tgcttcaggc gcgcgcgccg tccgatctgg
     3061 gcgggccgat caaggtcgaa taccagtacc agcagttctg acaaagcgga tctcccatct
     3121 catggaactg ttcgcgagct tcgatttctt ccaatacgcg ctactggctg gcctgggcgt
     3181 gtcgctcacc agccgcgctg tggcctggac ccggttgcca ctgcgctacg tgctggcctg
     3241 cctgggcctg ctgacggcgg cgctggtttt ccagaaccgg cccttgcagc cattgatcgt
     3301 gttcgccgta caagccgtgg cgctgtgcct gtgccgccgc aagatcatcc ccacgcgggc
     3361 cgccgtggtg ctcaccatgc tgccgctgct tctggtcaag acacatgccg tgggcatgct
     3421 gggtatgctc ggactgtcat tcatgacatt ccgtgcggtg gacgtgttgc tgttcgccca
     3481 cgacaacgaa cgcatccatc ccctcgacta cgtcacatac ttgtttttcc cgctcacgct
     3541 gctcgccgga cccatgtacc gctggcgcaa cttccagacc gacctgaatc atggctatgc
     3601 ccaggtcgac atgagccact ggcgcgaggg tttcgagtac ttgttgctgg gcctgaccca
     3661 gaaattcgcc atcgcgcatc tggtctggat cgccgggttg gcgcaggtgg cccccaacga
     3721 ctacagcttg cggggcgtgg tgctgaacac cgtgctctac agcgtctacc tgtatttcga
     3781 cttcgccggc tactcgaaca tggcggtggg tctgggcaga atgttcggcg tcaacctgcc
     3841 ggcgaatttc aacaaccccg tcctggccag aaatccacag gatttctggc gacgctggca
     3901 tatcagcctg tcggagtggc tgcgcgacgt ggtcttcatg cccatataca aggcattgtc
     3961 caggaacacg tacttctcgc ggcaccgttt cgccgcccag aacatcggca tcttcgccac
     4021 gctgttctgc atgggcatat ggaacggcct gcaatggcac tatatcgtca gcggcatcat
     4081 gttcggcact tactcggtga tccataaccg gctgctgctg gccgcgcgcg agcatccgcc
     4141 gctgcagcga tggctgtcaa cacggaccgc gcgcctgttg ggccggtttc tcacgctggc
     4201 ctcggcgatg ctggctctgt acgtgttcag tggccgcagc ccggtttagt gccgtggatc
     4261 atgaatatga tgtcatgggt atacacgcag ggtagcccgc caagtcgaca gtaagcacca
     4321 aggcaaacca caaggatttc gtcatgcacc tcgatctgcg cacaatgcga ttcgccgaca
     4381 ccgacctgga ccaagatcgg caggcggtgg tcggctgcga tcggagcctg agctggcaaa
     4441 ccttgaagga agaggcacag cgatggctgg gacgcgccat cgaactgggc gcgcggcccg
     4501 atgtgcccat cgtgctgtca ggccacaagc aggcggcttt cttcaccgcc atgacggcct
     4561 gcctgatatt gggagcgccg ttcgtgccgg tggacagcat ttatccggac gagcgtcgcc
     4621 agcgcatcgc cgaactcgtc ggcgcggcgc tgcactaccg ggccgacacc gacaccttcg
     4681 aacccaccgg gcaagccgcc tccccgctcg aggaaaaagg gctggcctac gtgctgttca
     4741 cgtcgggcag cacgggtgaa cccaagggcg tgcagatcgg ccgcgaaagc gttcaattgc
     4801 tttcacaatg gatgcggaca cgtttcgggc tgggcgaagc cccggtcttc atgaaccagg
     4861 cgccattcag tttcgatctg tcgatgttcg aagtgttcgg cacgctcggc agcggcggaa
     4921 cctgcgtgct gaatgcgcgc gagctgatcc aggacggccc ggccttcctt cagcggcttg
     4981 cgcgggagcg catcacctgc tgggtgtcga cgccatcatt cgcgcaccag caactgctga
     5041 acaagacctt ttcaggcacc gatctgccca cagtgaacac attcctgttc tgtggcgaag
     5101 tgttgcctca tgcgctgtgc gtccggctga ggcaacgctt cccgcaggcc cgcatcctca
     5161 acacctacgg ccccacggaa gccacggtcg ccaccacgct cattgaagtc gaccaggccg
     5221 tgttggacgc gcacaaggtg ttgccggtcg gttacgccaa gccggagtgc ctgattgaaa
     5281 tccacgacgg cgagatctgc gtcgtcggcg atcacgtcat gcgtggctat ctgaaccaac
     5341 ccgacctgaa ccaggccaga atgttccgtc acacggatgg acgacgcggc ctgcgcaccg
     5401 gcgatctcgg ccatctggat gccgacggcc tgctgtggtg ccaaggtcgg cgggacgatc
     5461 aggtcaagct caatggctac cgcatagaat tgcccgagat cgcgttggcc cttcagtcgc
     5521 tcgacgaggt gaagaacgct gtttgcctgg cgctgcggcg cccggacaac agcgtggcga
     5581 ggctggtggg tttcgtggaa ttgacggaac acggccggcc ttccggcgcg atcgagtggg
     5641 cgccctatcc ggcaaccaat tggaaacccc cactggaacg acgcctgcca ccgtatatgc
     5701 tgccgtcgga aatcatggtg gtggatgcgt tcccgatgtc ggtcaaccag aagatcgacg
     5761 gcaaggcgct gttggaaaca tatctgggaa aataggaaac atgcctggag aaacaggcgg
     5821 ccatggcgcg agccagccgc cctggatgcg cttgcctgcc caggttggac gtgggttggt
     5881 gccaagccgt cagaacaacg ccaactgggc gcttccccgc gttggtcgac ggaaatgcga
     5941 cacatccagc gcaacgcgcc gctgagtcaa tccggctttc tgacacgctt ttcgaaaccg
     6001 ctgctcgatc aggccggcca ccaccccggc gccacgcatc cgggtctgga aattcgcatc
     6061 gtataccttc ccgccacgcg tctgccgcac gaggttcatg acatgcgcct gtttcagcgg
     6121 atgatgcgcc gccagccatt cctggaacaa cccggcgatc tccattggca gccgcaacag
     6181 cacatagtgc gcgcttcgcg cgccctcggc cttggcgcgg aaaatgatgt tttcaagatc
     6241 atggtcattc agcgagggaa tgacaggcgc gacgatcaca ccggtgggga tgcctgcgtc
     6301 ggacagcgtg cgcacggcat cgagccgcgc ccccggcgtg ctggcgcgtg gttcaagcgt
     6361 tcgggcgatg tgccggtcga gcgtgcccac agacacgtga acacagacca gattccgggc
     6421 ggccatgcgg gcgagtagat caaggtcttg agtgaccgac gcggacttcg tggtgatggc
     6481 gacggggtgg ttgaactcat cgagcacatc caggatagat gccgtgatgc gaagccgccg
     6541 ttcaacgggt tgatacggat cggtgttggc ccccagcgcg atcaagcggg gtacgtacga
     6601 cgggttggac aaagtcttgc gcaacacgtc ggccgcattg tgtttggcat acagccgggt
     6661 ttcgaaatcc agccccgggg aatggccaag ataggcgtgc gacgggcgcg cataacaata
     6721 gacgcagcca tgctcgcaac cccgatacgg gttgatggac tgatcgaaat acagatcggg
     6781 cgaatcgttg cgcgagatga tggagcgggc ggtttcgtgg gtgacggtcg tggccggtct
     6841 ggattcttcg ggcctgtttt cattcaccca gccgtcgtcg aacgtttccc gctggagcat
     6901 gtcaaagcgc gaaacgtcgt tcgacaagct accccgaccc ttgattggcg cgttgtgcat
     6961 ggtggaatcc cttttttata ctgtacataa taacagtatt gtgagtctgt ctatcgatgc
     7021 gacgacacga caggcgtcac accagactgg aacacttaac caccgcccgg cgccgtccta
     7081 tccgcttacc aggagaacaa aatgacgatt ggatccacat tgcgcgcggt ggcgctggcc
     7141 ggtgcctgcg cctcgatgtt atcggcctgt gtcgtcgagc ccgtgcggcc gccccgcccg
     7201 gcgccagtca tggaaatcat ccctccccct cccgccgccg gctaccactg ggtcacgggg
     7261 cactaccgct ggcggcgcaa ccattggaaa tgggtgccgg gtcattggaa atacatgaga
     7321 tgacgcaact ctgacaccac tttcacgctt ggttacgtta ttatcatggg cgtcacgccg
     7381 cgtacggcgc gacgccaatc aaggaagagg aacactgttc gatgcgagca ctgctttacg
     7441 tgctgcccct gctgctgttg gccggctgcg ccacaactgg cgcgccaccc tccgaaccga
     7501 acaacctgtg cgccatcttc cgccaaaggc cggcctggta tcaggcggcg atccatgccc
     7561 aggaaaaatg gggggttccg ccccaggttc ccatggccat catgtaccag gaatcaagct
     7621 tccgggctaa agccctgccg ccccgttact atgctctagg cttcattcca tggggccgtg
     7681 tcagttctgc ctacggctac gcccaggcca aggacgacac ctgggccgat taccaacgcg
     7741 acgccggcag ttggttctct gaccgcgacg acttcgacga cgccatggat ttcatgggct
     7801 ggtacatcag caagacccag aaaatagacg gcgtgtcgaa atgggacgcc tacaaccaat
     7861 acctggccta ccacgagggc tggacgggtt atcggcaacg cagttacacc aggaagcctt
     7921 ggctgatgcg tgtcgcgcga cgggtacagg aacgcgccga acgcttcggc gcgcaatacc
     7981 ggggctgcga gaaacaactg agcgccgccg acgggtcgtt cggctgagcg agccggcgaa
     8041 ccatcaactc gcgacccgat atgctgtttg gcgcgcgcag gcaaatcaac caaacggcac
     8101 gccgggtatc cggcgtcaaa cgccttgccg ctgccgcggg gcgctctgcg aactggcctg
     8161 cgacagcgcg gcacccggtt cgacgtcttc agtgacccac acattgccgc cgatgaccgc
     8221 ccccttgccc agggtgacgc gtcccagaat ggtcgcgccc gcgtagatga ccacgtcgtc
     8281 ttcgacgatg gggtggcgcg gcaagccttt cttgagactg ccgtccgcat ccgccggaaa
     8341 ccgcttggcg ccgagcgtga cggcttggta gatacgcacg ccagtgccca gccgagcggt
     8401 ttcgccgatg acgacacccg tgccgtggtc gatgaagcaa cttgcgccga tctgcgcgcc
     8461 gggatgaatg tcgatgcccg tttcactgtg cgcgatctcg gtgatgatgc gcgccagaag
     8521 atgcaatccc gacaagtaca actgatgcgc cagacgatag tgaatcaagg cgagaatacc
     8581 cggatagcac agcagcacct catcgacgct gcccgccgcc ggatcgccct gataggccgc
     8641 cagcacgtcc tgatcgagca ggcggcgtat gccgggaagc gactccgaga accgattgac
     8701 tatttccagc gcacgctgtt cgatgcctga cgcatccgcg gcctcgtgac gcgcctggta
     8761 gcgcaattca agcttggctt gcgtgaacag gctttgcagg gcacagttca acgtatagcc
     8821 cacatacagg ttctcgccgt catgccgcag gttctccggc cccaggcgca tgggaaacag
     8881 cgcccccttc aactggtcga cgatatgggc cagcacatcc cgcgaaggga attcgcgcgc
     8941 atgcggctca cgcgcccggc gctgggagtc acgccatgcc ccgcgtatgg agtccaactc
     9001 ctggacgata tccccgacat cgaaaactgc catgcgacta ctcccaacct gatcgaagaa
     9061 cggccgcgcg cgggcgcggt ggggcacgcc accactcacg cgcgcgaatg gaaactttgc
     9121 agaataaaac tccgcatcca cgcagtaaac taccaaataa tataaatcta ataacctcca
     9181 gtattaagca ttctctggac aagccgcggc gcctgcttgc cgtcgacttc aaaggatccc
     9241 ttgaagctca gcaaaccaca gcgccagccc tccaattgat tcggccggag gatcaggcct
     9301 tataaaccgg cgcggcgggc gaacccgcga tccgtgcccg accgaaccat accgagacca
     9361 gcaacagcac caatccaccc aacgccaacc ccgaccccac cagtcctgtg gacgcccagc
     9421 ccaggccgtg cgcgatggcg atgcccccta gaaacggccc cagcgcattg gcgaagttga
     9481 aggcggagtg attcaaggcc gccgccaaac cctgggcatc cctggcaacg tccattagcc
     9541 gagtctgcaa tatggtgccc agggcgccac caacgccaat caggaacacg tcgacactta
     9601 cggtccacgc attgccggcc gccaagggga aaaacgccgt cgccagagcg ctccagacca
     9661 acagaccgcc ggctgtgggc atcaacgcgc ggtcagcaaa ccatggcaca acgatgttgc
     9721 cgacagtcat ccccaccccg aagaccccca gcaccaatgg gatgatgccc gcggaaaccc
     9781 ccgtcacatg catgagcgtg tcggcgagat aggtatagac cgcgaacaga ccgccaaagc
     9841 cgattgcgcc agtggccaac gtcaaccaca cctgcggctg cctgagcgcg cccagttcgc
     9901 gcagcggact ggaacctggc tcggccggcg agcgcggcac cgtccagccc accatgatga
     9961 cggtcagcac gccggtgaca gccaccagcc cgaaccccca gcgccagctc acagtatgcc
    10021 ccagccagtt ggcgaacggc acgccgatga tggtcgccgc ggtcaggccc aggaacatcc
    10081 gccccaccgc ctggctgcgt ttgttcaacg gcaccagtga cacggcgacc agggccccga
    10141 taccgaagta cgcgccatgc ggcaggccac tgagaaaacg cagcaccagc agccagtgat
    10201 aggacggtgc gaaggcgcac aaaccgttga acaacgaaaa catcgccatc agccaaagca
    10261 gcagtgtgcg gcgcgccatg cgcgcggcca gcacggtgag gaccggcgcc cccacgacaa
    10321 cgcccagcgc atacgcgctg atgacatgcc ccgccgttgg cgcatcgatc ttcaggtcga
    10381 cggcgaaata cggcaaaagg ctcatcgtgg cgaactcagt ggtgccaatg gcaaacgccc
    10441 cgatggccag agcgaacaga accagaccgg ggggatgtgc caccggtgtg cggcgtgagg
    10501 gtgaatgatg catgctgcgg gggaggaagt ggccgatgac gaccggaaga ccgtcgcgac
    10561 agatgtgcgc ggcgtcggag atgttgtggg caaagcgtca ttttattacc gaccagacga
    10621 tcatgcgttg gatcatgcaa tgtcggcatg accaaatcgc ggcggcataa ggtataaaat
    10681 cccattcaaa catctcatcg cgagttcggg tacggcaagc caggccgtcg ccgtgtctta
    10741 tacacttgct caatatggcc tgacgtggcg gttcgcttct tatgatcaaa tggggtctta
    10801 tcgcgctctg gatcgcgtca atcacgtacg tgtatttccg cggccggatc cgggtcagtt
    10861 ttctgcgcac cgtgctcgat cactcggccg tgctggcacc ggtcaacgtg ttcatgtatc
    10921 tgttctcggc ggtgcccgcg cggccgttca tcccggttga gcatctggcg ggcctgcgcg
    10981 ctctcgatga caactgggaa accattcgcg acgaggccaa gcgcctgcac gacgcgcagc
    11041 agatccgcgc cgctgaaaag aacaacgacg cgggattcaa ctcgttcttc aagactggct
    11101 ggaagcggtt ctacctgaaa tggtacgaag ccgaacaccc ttcggccgcg cagctttgcc
    11161 cgcaaaccgt gcgcattctg cacgacatcc cccaggtgaa agccgccatg ttcgcggtgc
    11221 tgcctccggg gggcaagctc aacccgcatc gcgatccctt cgccggatca ttacgctacc
    11281 acctgggcct ggccacgccc aacgacgacc gctgcttcat cgaggtggac ggcgaacgct
    11341 acagttggcg cgacggcaaa agcgtggtgt tcgacgaaac ctacatacac tgggccgaga
    11401 acaccagtga ccagacccgc atcatcctgt tctgcgatct ggaacgcccg ctgaggtttt
    11461 cgtgggcctc gcgcttcaac cgttggttct cccggatttt catgacattg gcgagctcac
    11521 ccaatcagga cggagaccag accggcttca tcaaccggct gttcttcatc gcctggaaga
    11581 tcggccaata ccggcgtcgc ctgaagcgct ggaaccgcac ggtctatcag ggtgtccgag
    11641 tggcgctggc catcgccatc ctggcgttga tcgtctacat ctgaaccgcg tgtcgctttc
    11701 gagcgacgcc gccttccagg cccgccgtca gtcctgtgac tgaaccccgg ggccatgtgg
    11761 catcccgggg atgccgatta cggcagtgca caatgaccgt gttccgcgca acacgggttt
    11821 tcactgtatc tccacctcag tcattcagct ataattcaac aaaatttcag ttggcattca
    11881 gattatgtcc aactgaggtg cggttctcac attcagcgcc gtcacatgga aaaagggcca
    11941 tggacaggcg ccagttttag tgggaagagg acgaacgcat ggtcctgcgg ggccatgcgt
    12001 tcaatttttt gcataaagac taaaagaggg gaatgtcacg atgaatctgt cttcatccag
    12061 gctctggacc gccatgggct tcacggtcgc cttgtccttc gccggtcagg cgttggccga
    12121 ctcgcccatt gtcatcaagt tcagccatgt ggtggccgat gccacgccaa agggccaggg
    12181 cgcgctgaaa ttcaaggaag tcgccgaaaa actcctgccc ggcaaggttc aagtacaagt
    12241 gttccccaac tcgcaattgt tcggcgacgc caaggaaatg gaagccgtgc tgctcggcga
    12301 cgtccagttc atcgctccgt cgctgtccaa gttcgaccgc tatacgaaga aggtgcaact
    12361 gttcgacctg cctttcctgt tcgatgacat cgccgccgtc gaccgcttcc agcacagcga
    12421 tgccggcaag gcgctgttga attccatgca gaaccgcggg ctgcaaggcc tggcctactg
    12481 gcacaacggg ttgaagcaac tttccaccaa caaagaaaag ctcacacgcc ccgaagatgt
    12541 cgcgggcctg aagttccgca tccagaactc cgacgtgctc gaggcccaat tccgccaact
    12601 gaaggcgaat cctcagaaac tggccttcag cgaagtctat caagccctcc agacaggtgt
    12661 ggtcgacggc caggaaaaca cctggtccaa catctattca cagaaattcc acgaagtcca
    12721 gaaaaccatc gccaacacca accatggcgt catcgactac atggtggtca ccaacgccaa
    12781 atggtggcag gggctgcctg aagacgtgcg cgccggcctg acacaggcca tggaagaggc
    12841 caccgtctat gccaacacgc gcgccgctga actcaacgac cgcgatcgtg aacgcatcgc
    12901 ccaggcgggc ggcgccaaga tccaggcttt gtcgaaagaa gacaaagccg cctggcgcca
    12961 ggccatggcc ccggtatggc agaaattcga aagccagatc ggcaccgacc tgatcaaggc
    13021 cgcgcaggaa tccaacacct gaaacccaaa ggttctctga accctgacgc ggcgggtgtc
    13081 gacgcccgcc gccgctggag caatacccca tgaaatccgc ctggttcggg cgactggagg
    13141 aaggcctgat cgcccttctc ctggccgcca tgacgctcat tacttttggg caggtcgtgg
    13201 cccgctactt gttcaactac agcttcgtct gggccctgga actctccacc tacctgttcg
    13261 gtggcctgat tttcctgggc atgtcgtacg gcgtgcgcgt caacgcccac ataggcgtcg
    13321 acgcgctggt caaactcctt ccaaaaccca cagcccatcg gctcggtatt gtcgccgtcc
    13381 tgctctgcct ggtgtacgcg ggcatcgtgt tcgccggggg ctgggtgtat gtcgccaaga
    13441 tccagcaaat cggcattctc gcgcaagaca tccccatccc ccaatggatc ccgcgggtcg
    13501 tcctgccact ggggttcgcc ctgctgttct tccgcttcac cgagattctg gtccgcatgc
    13561 tccgtggcca ggaaacacac ttgctgggcg atgaagccga tgacgccttg aagttgcgcg
    13621 ccgacgccaa cgcgccggca acccccgaag ggaagaaagc atgaccacaa ccgcgctttt
    13681 cctcatgctg ttcgtcttca tgttcatggg tctgcccgtg gcggtagcgt tgggtctgtc
    13741 gtccatcctg accatcctga cattcggtca cgactccctg gcatcgctgt ctctgaaagt
    13801 gttcgaaacc tccgaacact tcacgctgat ggcgatcccc ttcttcattc tcgcgggcgc
    13861 cttcatgacc acgggcgggg tggccaagcg catgatccgc ttcgccaccg cctgcgtcgg
    13921 gcatctgcac ggcgggttag ccatcgcatc ggtcatggcc tgcgtgctgt tcgccgccgt
    13981 atcgggttca tcaccggcca ccgtggtcgc tgtcggctcg atcgtgatcg cgggcatggt
    14041 gcgggccggc tacccccagt cgttcgccgc cggcgtcatc tgcaacgcgg gtacgctggg
    14101 tatcctcgtc ccgccctcca tcgtcatggt ggtgtacggg gccgccacag agacatccgt
    14161 ggggcgcctg ttcatggcgg gggtattgcc cggggcgctg ctgggcctca tgctgatgac
    14221 cgtcatctac ctcatcgccc gggtccgcaa actgccacgc caaccacgcg ccagtctggg
    14281 cgaagtcttt ggcgccgcgc gtgaatcgtt ctgggggctg atgctgatcg tcatcatcct
    14341 ggggggcatc tacggcggga tattcacccc caccgaagcg gcggcggtgg cggcggtgta
    14401 cgccttcgcg gtcgcggtgt tcgtctaccg cgacatcggc atgcgccagg tgcccggcat
    14461 cctgatcgat gcagccaagg tcaccgtgat gctgatgttc atcatcgcca acgccctgct
    14521 gttcgcccac gtgctgacca ccgaacgcat cccgcagatg atcgccgaac acatcgttgc
    14581 ctggaacatg tcggcctggc agttcctgat cgtcgtcaac atcctgctac tgatcgccgg
    14641 cgccttcatg gaacccaccg gcatcatcct gatcctggca cccatcctgt tcccgatcgc
    14701 cacgaaacta ggcatcgacc cggtgcacct gggcatcatc atcgtggtca acctggaaat
    14761 cgggatggtc acaccaccga tagggctgaa tctgttcgtc acggcgggca tcaccaacat
    14821 gaacatgggc caggtgatac gcgccgccgt gccctggctg tcggtgctgc tggtgtttct
    14881 ggcgttcatc acctacgttc cgcaggtgtc gctgtggttg cccaacatgg ggttcaaata
    14941 aggtacacgt cgatacaaga acgtaaccag gcatggcggt tccggcgtgg ttatttcaac
    15001 ccgatacagg ttcgggccaa aggcttgcac cgttcgcgca cgccccgtac tctggcgcca
    15061 accaagtact tatgcggagg ccaaccatca tgaacctacg acgtcatctc acccagatcg
    15121 cggtttccag cgtcgtcacc ctggcggtat gcaccactgc ggtggcttcc gaacggcgga
    15181 ccgccaacac cgtactgggc gcgggccttg gtgccgtggc gggcgcggtg ctatccgacg
    15241 gtgacccctt gctgacgctg ggcggcgcgg cggccggtgg cctgttgggc aacgtgctga
    15301 ccgaagaccg gcgccattat cacgggcgct acgacggcgc gcaccgccgc cgcgtggaag
    15361 agcggtacgg ctatcaccac gagcgccgcc gcggatacga ccggggttgg agacgatcgc
    15421 accgcggtca ctgattaccg ccgaccggat tcggcacgat cagcatccag tgcacaccga
    15481 accggtcggc caccatgccg aaatgcgatg caaagaacgt ccgatccatc ggcatggtta
    15541 cctggccgtc ttttcccaga gcggcgaaca tgcgctcggc cgcagccccg tcgggagccg
    15601 tcagcacgat cgacacaccc tggaatttcg gttcgcccga gcacatgccg tcggacgcca
    15661 tgagcatggc gtcgcctatg cggaaactga catgcatgat cttttcctga ttctcgggcg
    15721 atccgctgca cgcgccattt gcctcgggat tgtccttgaa ccgcaagagc tggacaacct
    15781 cagcgcccag cgactcttcg taaaacgcca gggcttcctc gcaccggccc tcgaagatga
    15841 gatagggatg tatttccacg acgcctccag aacaggggaa gtgacagaca acggccggat
    15901 cacggccaat cggaagacgg tttgaacaga attgtgtaca ccgtccactg acatcataat
    15961 ggaacaggtg tacagtgtcc acataaaaat gtcactgata caaaaaacaa tggatagccc
    16021 acccgatgtg tccgctcgaa cgctgaagcg ccccaaagaa cgcggcacat accggcacgg
    16081 tgacttgaaa cgcgccctgc tggaagcggg gatggaactc gcgcggcaag gcgggcccga
    16141 ggctgtcgtg ctacgcgaag ccacgcgccg cgtgggggtc gtgcccaatg ccgcgtatcg
    16201 tcacttcacc aatagagatg cgctgctgca ggccgtgcgg gcggcggcgt tgtcggcgct
    16261 cgcgcaagcc atggaaaacg agctggcccg gatcccggcc gaacttccct cttccgcacg
    16321 tgcgcgcgcc agcgtgcgcg ccatcggcac cggctacctg actttcgcac acgaagaacc
    16381 cggtttgttc cgcacggctt tcgcgatgcc cgacagcgtg gaagacgact tggatccggc
    16441 caagacaggc cgaagcgggc tcaatccttt tcaactgctg ggcaaggcgc tggacgaact
    16501 agtgaccgct aaaacactgc ctgcaacgcg acggcccggt gccgaatacc tggcttggtc
    16561 ggctgtccac ggcctggcca tgctgatgct ggaaggcccg ttgcgcagct tggcgccggc
    16621 tcaggcccat gccgttggcg agcatctgct agatatgatc gaacgcggct tgtaatcgcc
    16681 gcgccaatcc ctgctcatcc cgcgcgacaa ctgcacgcgc cgtcttgttt atttagctac
    16741 atttgatata atttcatatg taattatttg gtcgccaaca accatcctgt ccgtgagcca
    16801 ctcttgaagg cggggcaaaa ccggtcgaaa atgacgctgc cgaccgtacc gaggactcgc
    16861 ccatgtcatt cgaatctcgc ataagccggt tttgcaagac ccatcccaac gctccgcgcg
    16921 aacacatcct cgcctcgcga ttgctgttcc gcaccgcccg tctgttgcac gaacgcatag
    16981 acctcgcgtt ggctccgttt tcgttgaaca tgtcgcaata cctggtgttg agcatgctgg
    17041 ccgccgacga gggcgaaccc agttcacctt cggaagtggg cagcacactc gatgccacac
    17101 gcacccagat gacccgcgca ctggacgctc tgcaggcaca gggcctgatc cggcggcgcg
    17161 ccagcgcaca ggatcgccgc agcctcgaac tcgaactcac cgaagccggt cgccaaatgg
    17221 tggaaaacgc cgcccccgca gtgcatgcgg tctacgcaca ggcctggtca gcggtgaagc
    17281 cggctgatcg caccgtcacc acgtcgagct tacaacgact gcacacaggg ctggagtcca
    17341 cgcagccatg aatcaccccc tgcgtcggaa aatgcggcca cgccggctac ttggactggc
    17401 caggctgctg tcgcaccggg agcggcaact gatctcgatg atgctgctgg cactcatcgt
    17461 gtcggtggaa ttcttcgaga acgtcatgtt cctgttttcg gcgagccacg tgatgggcgg
    17521 catcggcgcc gatccacgtg gtttcgcgct ggccatgtca acctatgccg tgggcagcct
    17581 gctcatgatc gtgaatcagc agtggctcgc gcagcgcttt ggctaccggc gatacctgac
    17641 ggtggcgttg acgttgttca caatcggcac gctcatggcc gcatccagca ccgacctgac
    17701 acaactggtg atcgcccggt tcgtgcaagg ctttggcggc ggagccctct tcaccagcag
    17761 tcgcgtgctg atccccatca tgttctcacc cgccgaccgg cctcgcgcgc aacgcatctt
    17821 catgatcggc atcttcggcg ccacagccat ggccccggcc atcgcggccg agctcatgga
    17881 tcacggcgtc tggcaggacg tcttctacgg ggccgtgcca ttgtccgtga tcgccgccgt
    17941 gggggcatgg cggctattgc ccgacgccga accccgggaa cgcacgcacc agccactgct
    18001 cgttccgctc ctgtggttcg gcctggcggt tgccgcgcta caggccgcgc tgaccgaggc
    18061 gcgcttcgac atgttctcgc accctgtgcg ccctgcgctc atcgccatag cgggcgcgct
    18121 gttgctcatc ggcttcctgc atcatcaatg gcatcatcac cagccgttgc tgcaattgca
    18181 cgcgctgcgc aaccccgtct acctgacggg gctgtcgttg tacttcatgt actacctgat
    18241 cagctacttc aacagctacc tgttccccat ctatgccgag cacgcactgg gtctgccact
    18301 atccacggtg ggatggctcg acacgtcggc ggggctgatg agcttcgtgg gcattctggc
    18361 ctatatacgc tacgccaaac tgatcaagag caagaagcca ttgatggtcg ccgggctgct
    18421 catcatggcc tgcgcggcct ggcgattctc cagcatgccg cccgacgtca cgccctggga
    18481 tctgattccc agcctggcca tgaaaggttt gttcgccgtg ctgttcgtca tccccgtggc
    18541 cggcttgacc tataagaacc tgaccgataa caccttcgcc catggctacc agagcaagaa
    18601 cctgatgcgg caggtgtcgg gctcactggc cccggcgcta ggcgcgattc ttcttcagaa
    18661 caggcaattc agcgtacaca cctcactggt ggacagcacc ggcaaccagc ccggcatcac
    18721 catgggctgg ctgaatcagg tgcagaccgc cctgatcgca cacggcatgg acgcgggcag
    18781 cgccgcccgg gtcgccatgg cccagctcac cggcatgctc gaccgtcagg ccatgctcat
    18841 cgccagtgaa gacctctacc ggctgatcat ggtcgtggcg atcgccggcg cctgcatcgt
    18901 catggcccag cggcggctgg cctgatcacg cgcaccggaa tgcccgggag gaatgatcga
    18961 caagttgtcg tatcattcaa gcagacacaa cctttccgag cgctgcggcg cgtcgaacgc
    19021 gccgctcccc cactcccgag gtccgatgcc gctgacattg atcctggccc tgccgttcgc
    19081 cggcagcgtg ctggccgcaa ccctgccgtc caacgcccgc aaccaggcgg cctggctggc
    19141 cggactcatc gccctggtgt gcgctgtcct ggtcgccgtc cagttcccga tcgtcgcgca
    19201 cggcggggtc gctcaaacca ccctcgcctg gctgccatcc ctggggctga acctcgtgtt
    19261 gcgcatggac ggctatgcat ggctgttcgc gctgttggtg gcgggcatgg gcgcgctcat
    19321 tgtcctgtac gcccggtact acatgtcgcc ggacgatccc gtaccccggt tcttttcctt
    19381 cttcctggcg ttcatgggtg ccatgctggg cgtggtgctg tccggcaaca tcattcaact
    19441 ggccatattc tgggaattga ccagcctgtc gtcgttcatg ctgatcgcct actggcatca
    19501 tcggtcggat gcgcgacgcg gtgcccgcat ggcactcacc atcaccgtcg ccggcgggct
    19561 ttgcctgctg gctggcatgc tgctgctggg gcgcatcgtc gggagctatg acctcggcca
    19621 ggtcctggcc tcggccaatc gcatccgcgc cagtgcctgg taccccaccg tgctgacact
    19681 cgtcgcgctc ggtgcgctcg ccaagagcgc gcagtttcct tttcatgtgt ggctgcccaa
    19741 cgccatggcg gcgcccacgc ccgtgtcggc ctatctgcat tcggccacca tggtgaaagc
    19801 cggcgtcttt ctgcttgcca ggttctggcc cgtgttgtcc ggcaccgatc aatggttctg
    19861 gatcatcggc ggggcggggc tgtgctcgct ggtgctgggc gcgttcgccg cgatgttcca
    19921 gcaggacatg aaaggcatat tggcctattc caccatcagt catctggggc tgatcaccct
    19981 gctgctgggc atgaacagcc gtctcgcgct ggtggcggcc atcttccaca tgatgaatca
    20041 cgccaccttc aagtcatcct tgttcatggc caccgggatc gtcgaccacg aaaccggcac
    20101 gcgtgatctc gaccgtctct cgggcctgcg ccgcaccatg ccagtcaccg ccaccctggc
    20161 catggtggcc gcggccgcca tggctggtgt gccattgctc aacggtttta tttcgaagga
    20221 aatgttcttc gcggaaaccg tgttcatcag cgggcattgg atcacccgcg tcggtctgcc
    20281 tgtggcggcg gtcgtggccg gcgcgttcag tgtggcctac tcgctgcgtt tcatcaggca
    20341 ggtgttcttc ggacaaccca cgcaagacct gccgcatcgc ccccacgatc cacctctctg
    20401 gatgctgctg cccagcgcgt tgctggtgct cgcatgcctg gtggtcggcg tgctgcccgg
    20461 gctcacgctc ggcccactgc ttcatgccgc cgcgctatcc atcctgggtg ccgagcacat
    20521 gccatcctat gacctggtgg tctggcacgg tttcaatctc ccgttggcca tgagcatgct
    20581 cgccatgctg ggcggcctgg ttctgctcca tctgttccgt ggacgctacc gttcacgccc
    20641 cggcctcaca cccttgctgc acaggctcga tggccgacgc gcctttgaaa ccctgctcga
    20701 gtctctggat ggcatcgccg ccgcgctgtt ttcatggacg tggtcacccc gtctccagcg
    20761 gcaggtggct ctgatagtcg ccatcaccat cgccgtggcg ctgccgccgc tacgacatgg
    20821 ctggcccgcc ggcacattat cgacctctgt ggaaccgtca ttcatcatcc tgtggatagc
    20881 gggaaccgct tgcgccctgg gggcggcggc aacagccagg cgacaccgac tgacgtcgct
    20941 cgccctggtc ggcggcgctg ggctgtgcac gtgtatcacc ttcgcctggc tgtccgctcc
    21001 ggacctggcc ctgacacaac tagccgtcga aaccgtcacg acgattctat tgctgctggg
    21061 cctgcgctgg ttgccacaac gcgccgaaca caacgacgcg gcgcgcccca tgacacgtga
    21121 tctgcccggc atgctcctcg ccgtcgccgg tggcgcgggg ttggccacgc tggcctacgc
    21181 cgtcatgacg catccgcaga acccgggtat cgcctcgtac ttcatcgaac acgcgttgcc
    21241 cgccggcggc gggggcaatg tcgtcaatgt gattctggtc gacttccgcg ggttcgacac
    21301 gctgggggag atcaccgtgc tgggtatcgt cgccctcacc gtctacgcat tgctgcggcg
    21361 ttttcgccca tcgcctgaaa gcctcgccgt cacggccaca accgccatgc cggaggcgat
    21421 ggacgtgccg gccgtgctga cgcgactgct gctgcccttg gcgggcctga tatccattta
    21481 tttcctgctg cgcggccaca acctgccagg cggcggtttc gtgggcgggc tgatcttcgc
    21541 cacggccatc atcgtgcagt acatgacatc gggtatcgcg tgggtcgaat cgcgcccccg
    21601 cctgcaaccc caatactgga tcgccgccgg cctgctgatc gccggcatca ccgctgtgtc
    21661 ggcctggtgg gccgcgcgcc ccctgctgac cgcgctcacc tggaatcccg tccttcctct
    21721 catcggcgaa gtgcatatat ccagtgtgct gttcttcgac attggggtct atatgctggt
    21781 gatcggtgcc accatgctga tcctcatcgc gctggcccac cagtcgttgc gctttcaccg
    21841 cgctcccgcg accgaaccag cctccggcac ccaacccgca ccatccggag ccccatgatg
    21901 gagatcgtcc tcgccatcgt cataggcatt ctcggcggaa cgggcatctg gctgctgctg
    21961 cgccctcgaa cgttccagtt catcatgggt ttgtccctgc tttcctacgc ggtgaacctg
    22021 ttcattttcg cgatggggcg tctggcgatt ggcgccgctc cggtgatcgc gccgggcgcc
    22081 gccggccata ccgtgtacga cgatcccgtc ccgcaggccc tggtgctcac cgcgatcgtc
    22141 atcggcttcg cgaccacggc cctgttcctg gtcgtgcttc tggccgcccg cggtttctcg
    22201 ggaactgacc acgtcgatgg gcaagagcca tgatgcctgc tggttggtcg caccatctgc
    22261 ccattctgcc cattgtcatt ccgctggcgg ccggggcggc catgctaatg ctcaaagaat
    22321 cgaaccgaca ggcgcgcgtg acactggccc tggcgtccac attggcccag atcacagtgg
    22381 cggtggcgct gctgtgcatg acggcgggcg ccatccccgg cttcgggccg cacgatatcg
    22441 gggcctacct gctgggtgac tggccagccc ccttcggcat tgtcgtggtg gcggatcgcc
    22501 tcgcggccgt gctgctcacg ctcaatgccg ccatggggct gtgcgtcctg gtgtatgcct
    22561 gcgcccgatg ggatcgcgcg ggggtgcact tccacccgtt gttccagttc ctgctcatgg
    22621 gcctcaatgg cgcgttcctg acgggcgacc tcttcaacct gttcgtgttc ttcgaaattc
    22681 tactggcggc gtcctacgga ttgatgctgc atggctccgg cgcggcgcgc gtcggatcgg
    22741 ggctgcatta catcgccgtc aaccttgtcg cctcactgtt gctgctgatc ggcatcgcgc
    22801 tcatctacgg agtgaccggc acattgaaca tggccgatct cgccgcgcgc gcgggcgggc
    22861 tcgccaacac agaccggcgc ttcttcgagg ccggcgcagc catcctcggg atgggcttcc
    22921 tcatcaaggc cgccgcctgg ccgctgaact tctggctgcc gggtgcctat gcgtcggcca
    22981 gcgcccccgt ggcggcggtg ttcgccatca tgaccaaggt cggcgtgtac gcgttgctgc
    23041 gcatggggtc gctcttactg ccaacacagg caccggccgc attccgtggc acctggatgt
    23101 tttccatcgg actggccacg atggtcttcg gcacgctcgg tctgttgacc gcaagacgca
    23161 tcccgcgcct ggcggcctac agcgtcatca tttcgacggg caccctgctc gccgcgctcg
    23221 gtatgccggg catcacgctg actggtccag ccctgttcta cctcatcagt tccgtgctgg
    23281 cggtggcggc gcttttcctg cttgccgaga tggtcgaacg cactcagcca ttcggcgccg
    23341 gcctgctgac cgtgacccag gaagccttcc acccggacga agacgaaacc ctggatcaga
    23401 atccggacga ctcaggcatc gccattccgt cggccatggt gttcctggga ctgtccttcg
    23461 ccgcctgcgc attgctgatc accggcctgc cgccgctttc aggttttgtc gccaaattcg
    23521 cgctgatgtc cacgttgctg aacatgcccg gcaccacggc gccgtccctg gaggcctggc
    23581 tgctgttcac ggcgatcctg gtctcgggca tggctggcgt catcgccctg ggccgggccg
    23641 gcatccagac gttctggagt acggaggaca tcatcgtgcc tcaccttcgt ttaggcgagg
    23701 ccgcgccggt cgcggtgctc gtggcaggat gcatcgtgct cacactccgc gccgatcccg
    23761 tgctcattta tctcaaggcg acggcggcgt ctctcgacca tccggcgtat tacatcgagg
    23821 cggtgctctc ccagcgcccg gtggtgcgcc cacccgacgc tggatctggg gctggggctg
    23881 gagctgaatc tggggctgga gctgaatctg gagcccatcc atgatccgac gctgcctgcg
    23941 gcgtgccgcc ctaccgctat gccttcttgc cgcctggcta ttgctgcagg gcacgttcgc
    24001 ccttggcgaa gccttgctgg ggctcgctct ggcgctcgcg ctggtacgcg ccgcagcacc
    24061 cctgtgcccg cgacacgcct tcccgcgacg gcctcgtgtc atgctgggtc tgctcggtct
    24121 cgtcctgctc gacatcctgc aatcgaacgt catggtggcg cgcctcatct ggtcgggtcg
    24181 gcgccgacgg ccaacacccg gattcgtccg cattccgctt gccttgcgcg acctcaacgc
    24241 actggccacc ctggcctgca tcatcacctg cacgccagga accgtctggg tggacctctc
    24301 acccgatcat gtgctgacac tgcacgtgct ggacctggac aacgaatcgg cctggatcca
    24361 gaccattcag caacgctatg aatcccgact gaaggagatt ttcaaatgcc ccacctgatc
    24421 gactgggcct gcgcgttcag cctgggctgc ctggccctgg gtctggcttg cgctggctgg
    24481 cgcctgctgc gcggccccac gccgcccgat cgcgtcctgg cgctggacac gatgtacctg
    24541 aatggcatgc tgatgattct gatattgggg ttgcgcatcg gcaccgatct gtacttcgac
    24601 atcgcactgc tgatcagcct gctgggcttc gccagctcca tggcgatggc gaaatacctg
    24661 ttgcgcggag aggtcatcga gccatgaaca ccctgcccct ctgggccggg attccggccg
    24721 cgatcctgat cgtgctcgga gggttgatcg ccctgaccgg gtcgctgggt ctgctgcgtt
    24781 tcagcgaatt ccacgcccgc atccacgcac cagccatcat caacacgctg ggcgcgacct
    24841 gcctggtcgc cgcctccatc ctgatatggt tcgcgctgga ttccggaccg atcttccagg
    24901 agattctgat cctgttgttt ctggtgatca cgtcacccat caccgccatg ctgctcatgc
    24961 gggcggccag ccggcaccat ggagacaacg aacaacgcca tgtcgactaa tacgcggttc
    25021 acccggaaaa ggccctgggc attcttctgt gaaatttctc gttacaccgc tgatcacgaa
    25081 cgatctatac tggtgacgag cctggaatga ccacggctcg gagtcaaagc agatcaagga
    25141 gatcgttcat gacagctccg tatttcgcat cacacaaaca cctggttgcc cgcctgttct
    25201 gtgcaacgct actggcaacc gccgcatccg ccgcgggcgc ctataccacg cacagcatga
    25261 atgtcagtcc ggaggcccaa taccaggccg acgtggcgca ttgcaagtcc ggccagtcca
    25321 atgaagacca agccacctgc ttgaaagaag ccggcgccgc gctcgaagcc gccaaacgcg
    25381 gcaagctgac gaccgccccg tcatacacca ccgaccagac aaaacgctgc caagaactac
    25441 ccgccaataa acaacaggac tgtctggcgc tgatgtccga ccagggcagc acccatgtcg
    25501 aaggcagtgt caatggtggt ggcgttctgc gcgaaaccac catcaccgtg ccagccggtt
    25561 cgcaataacc ctcggcgccg ccgcgtactc gcggcagcgt tgggcatgcc gctgccgtca
    25621 tagctcctgc tgcatgccca tttccaccac ccggttcggg ggaatcttgt agaaacgggt
    25681 gctgcgggtg gcgttgcgag ccatgaaggc gaagaggctg cgacgccaac gcaaccgcaa
    25741 gggtaaccgg cgcaccacga tcaccgtctg ccgtgacaga aaatacgatg tctgcatcgg
    25801 ctcgaggttg atctcggggt gtgcctgggt gatctgctcc agaacctggg gcacattggg
    25861 ttcttgcttg aacccccaac gcgcaatggc ttgccagctg gatcggctca gacgctccag
    25921 ctcgaaccgc tcgtcaaatg gcacataggg cacgtccgcg ctctcgaccg tcaggaacag
    25981 cgcctgttcg tgcaacacct tgttgtgctt gaggttatgc aacaacgcgg gcggcactgt
    26041 gccgtaaatc atgctcatga aaacagaagt gccttcgacc cgtgtggggg ggtattgctc
    26101 caggccttcc atgaatgact tcagcggtgg ctgatcgccc gccaggttgt ggtgcatttt
    26161 ctcgcgcccc tgcttccacg tcagcattag cgtcagcagg ctgataccga ccagcatcgg
    26221 cagccagccg ccttcgaaca gcttcgtggc gttcgaggag aaaagcagta tgtccaggat
    26281 caggaacacc gtgagcagcg gccaccatag atacttgcgc gggccgcgcg agcttctggg
    26341 aagcaccgcg aacgcaagca gagatgtcat cagcatggtt ccggtgacag cgaaaccgta
    26401 ggcctgggcc aggcgatcgg aagactggaa cactaccacc agcaccacca cggccacgaa
    26461 caacaacgag ttgacccggg gcatgtagat ctgcccttcc tcgcgcgccg acgtgtgctg
    26521 gatggccatg agtggccaga agcccagttg caccgcctga cgggtcacgg aaaacgcccc
    26581 cgaaatgacc gactgggacg cgatcacggt ggcgatggtg gccaacgcca ccagagggat
    26641 cagcccccac gatggcgcca tgaagaagaa tgggttgctg atagccgccg ggttggccat
    26701 cagcacggcc ccttggccga agtagcacag caccaggccg ggcatgacca cggcgaacca
    26761 ggctcggcgg atggccgtgc ggccgtagtg ccccatgtcg gcgtacaagg cctcggcccc
    26821 ggtcagcgcc agcaccaccg cccccagcag cagaaaggct tccaacggac cgtcggtgat
    26881 gagtcgccat gcatagaggg gattgagcgc cagcagtatc ttgggtgtca gccagatgcg
    26941 ccaggcgcca agcacgccaa gcgtgatgaa ccacaacagc atgatgggtc cgaagagctt
    27001 gccgactgtt ccggtgccgc gcgactggat caggaacaag gccgccagaa caaccaccga
    27061 gatgggcacg atcaggctat cgagctcgtg ggacaccacg ccaatgccct cgacggccga
    27121 gagaacggaa atggccggcg tgatcatgct gtcgccgtag aacagcgccg cgccgatcag
    27181 gcccagaacg gtcagcacca tgccacgccg ccgcgtcatg ccgcgcaccg ccaattcggt
    27241 cagcgccagc gtgccgcctt cgccac
//
LOCUS       DASTNK010000091        23828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1545269, whole genome shotgun sequence.
ACCESSION   DASTNK010000091 DASTNK010000000
VERSION     DASTNK010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 23828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23828
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545269"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..654
                     /gene="ahpC"
                     /locus_tag="VFR20_07035"
     CDS             91..654
                     /gene="ahpC"
                     /locus_tag="VFR20_07035"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HEU0230308.1"
                     /translation="MSLINTKIKPFTATAYHDGKFVDVSDSTVAGKWAVFVFYPADFT
                     FVCPTELEDLANHYAEFQKMGVEVYSVSTDTHFAHKAWHDTSDAIKKVSYPMIGDPTH
                     TLARNFDVLIQEEGISLRGTFVVNPEGQIKAMEIHDNGIGRDASELLRKVKAAQYIAA
                     HPNEVCPAKWEEGAQTLTPSLHLVGKI"
     gene            887..2479
                     /gene="ahpF"
                     /locus_tag="VFR20_07040"
     CDS             887..2479
                     /gene="ahpF"
                     /locus_tag="VFR20_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEU0230309.1"
                     /translation="MLDANIKSQLSTYFERIRLPLELRASLDDGGKSSELRAFLEEIA
                     GLSDKITLVDEQPRAARVPSFAIARIDSDVSVRFAGIPLGHELTSLVLALLQVGGHPA
                     KIEDAVVGQIRGLSGEYRFETYFSQSCQNCPDVVQALNAMSVINPAIQHVAIDGAFFR
                     DEVEAREILSVPSVFLNGELFHQGRAGVEELLAKLDTGDDTRRVDELNARDEFDVLVV
                     GGGPAGAAAAVYAARKGIRTAVVAERFGGQVLDTLAIENYLSVLETEGPKFGLALEQH
                     VKAYEVDIINLQRAAKLIPGKSIRVELASGATLKAKTVILSTGARWREINVPGEREYR
                     NRGVAYCPHCDGPLFKGRRVAVIGGGNSGVEAAIDLAGIVGHVTLIEFDQALRADDIL
                     QRKLRTLPNVDVITSAQTTGITGDGKRVTGLDYSSRVDGSVHHVDLEGVFVQIGLVPN
                     TEWLRGAVALTPRGEIEVDARGQTSVPGVFAAGDVTTVPYKQIIIAAGDGAKAALGAF
                     DHLIRSSVDEDEENVPAVANVV"
     gene            complement(2558..3604)
                     /locus_tag="VFR20_07045"
     CDS             complement(2558..3604)
                     /locus_tag="VFR20_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEU0230310.1"
                     /translation="MAFKKSALACTVLLAALGASGAALAESSVQLYGQIDAWVGAQKN
                     PGDINHTWVQGGGGMSTSYWGLKGSEDLGNGLKAVFKMEDFFRPQTGSSGRFDGDPFF
                     SRNAYVGLQSDDLGTLKMGRLTTSYFVSTILFNPFVDSYTFSPMVFHTYLGVNGQGIT
                     GDSGWSHAVMYTTPDFKGLTASFAYAFGNEPDQNGQNKWSANLLYFHGPFAATVAYQQ
                     VNFNNTPGDLGTVIPGFRKQRAVQVGMSYDLSFVKLFGQYQYIGNTIDGGNVTQNGGQ
                     LGVSVPLGGGNVLASYAFTRSTGASDVKRNTWALGYDYNLSKRTDVYVAYMSDHASDL
                     SSGSTYGVGMRMKF"
     gene            complement(3815..5218)
                     /gene="hydA"
                     /locus_tag="VFR20_07050"
     CDS             complement(3815..5218)
                     /gene="hydA"
                     /locus_tag="VFR20_07050"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEU0230311.1"
                     /translation="MNAEFDLILRNADVVTASDRFNCDIGIQDGKIAALGNGLGNASE
                     TIDATGLLTLPGGIDAHCHLDQPMPEGVRMADDFVTGTNSAACGGTTTIIPFAAQTKG
                     QSLRAAVDDYHRRADGKAVIDYAFHLIVTDPTPDVLANELPRLIEEGYTSFKIYMTYD
                     DLKLNDLDILRVLDVARAGQALVMIHAENADCIAWLTERLTSQGHSAPKFHAASRPMA
                     VEREATHRAITFSELAGVPILIVHVSGRDAIEQIRWAQARGLPIHAETCPQYVFLTEQ
                     DLDIEGYHGAKCVCSPPPRDKQNQDAVWSGLKQNVFSIFSSDHAAFRYNDPYGKMPGG
                     KEQPFQYIPNGIPGLETRLPLLFSGGVAEGRIDIHQFVALTATNPAKLYGLYPRKGSI
                     IVGADADIALWDPNRRVVIDNAMLHHNVDYTPYQGIEVKGWPVLTISRGKVVSRDLSL
                     TGRSGWGQFLPCGTPMM"
     gene            complement(5224..5961)
                     /locus_tag="VFR20_07055"
     CDS             complement(5224..5961)
                     /locus_tag="VFR20_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEU0230312.1"
                     /translation="MTKRTLLGMLTPSSNTTLEPVTSAIVSALPGVSAHFARFPVTEI
                     SLGERSQQQFDETRMLDAARSLADAKVDVIAWNGTSSGWLGFERDQRLCERITQATGI
                     PATTSVLALNEIFERRKVSDFALVSPYLAAVQEKIIANYLDAGVNCVAERHLDLHINF
                     EFSEVTDEQVRTMVMQVAEQRPQAITTFCTNLRAAHLAPELEALTGIPVYDTISTVVW
                     KSLSLAGYEIHGDIGWGSLFREPMHKA"
     gene            complement(5975..6646)
                     /locus_tag="VFR20_07060"
     CDS             complement(5975..6646)
                     /locus_tag="VFR20_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0230313.1"
                     /translation="MSNDQIYERIQIAILEHRLAPGTKLAEERLTEATGASRAKVRQV
                     LGRLAHEKVVTLIPNRGAFISRPTVQEARELFITRRLLEPGMVALLAENATKSQIQTL
                     RRSVALEQEARDAKDQHRIIRLSGEFHIHLADMAAKSFLPRIMRELTALTCLIITLYD
                     KPGTPACAHHEHGELVSLIETRDSNGARQCMLDHLNHIEQTLDLAAPEPSTPDFKAIF
                     HNEDK"
     gene            complement(6867..7622)
                     /locus_tag="VFR20_07065"
     CDS             complement(6867..7622)
                     /locus_tag="VFR20_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEU0230314.1"
                     /translation="MSKILLINPNISESVSDLIAAEARRTASASTHIDVVTAPLGVAY
                     IETRLEALLGAYAAADLAATQAPNYDALVIAAFGDPGLWGIRELVDIPVVGLTEAALM
                     SACMLGNRYSIIAISHRITAWYRECVQANGLLDRLASIRCLRQPLEDIGSVQSDHEGR
                     LRDLCDQAVREDGADVLILAGAPLAGLARTIRGDIPVPVVDGVSSAVRHAETLIALAP
                     GESRGGSFSLPPIKPNKGLSDAMRKLLAQGTLA"
     gene            complement(7652..8959)
                     /locus_tag="VFR20_07070"
     CDS             complement(7652..8959)
                     /locus_tag="VFR20_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0230315.1"
                     /translation="MGAQPQAVAAEFAVPQTRVRWKIFFLMLFLVTINYVDRASISVA
                     MPLIGKEFNIGPAFQGLVMSAFFWTYALMQIPGGMLADRHGPRVVISSATIFWGLFQA
                     LAAISTGWFGLLLTRLGLGASEAPIYPAGGKLNSIWMSQNERGRGATLLDGGAPLGSA
                     LGAILISFLIAMFDSWRTAFIIAGVGTMIAGLWSWHYIRNHPRQHPGVNDAEARHIEI
                     SHERELAAEPKVVSGRKLDFFKYRSVWGMFFGWMFFNTLFYGLLTWVPSYLSEARGLN
                     IKQMGGAVFIMFFAGFVGELVGGWLADKWREAGGSQGRVMRTLCGVAAILATVSIFSV
                     TQVSDPIMVVVTLSVTLFFLRWCGLYWCVPSILGTRNSVGFLGGTMNLGGNIAGVGVP
                     ILVGLIVQATGSFFWAFMLFAAAGVGLFVCSTLLIDYDKKLPI"
     gene            complement(9377..10714)
                     /locus_tag="VFR20_07075"
     CDS             complement(9377..10714)
                     /locus_tag="VFR20_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU0230316.1"
                     /translation="MRRSDVVEFLLMLATVVQWLVLASITGAIVGCGTSVFLHALFYL
                     TGKSANIPLWFQMLLLPTGGLLNGLLLYYGYKANTTGLKDSAIAAVHDQNGKMPFKTA
                     AIKPVAAIITLACGGSAGKEGPCSHIGASLAAGIGQLLKLNPELQRRLVACGVSAGFA
                     SVFGTPIAGAIYGVEVLSIGRIRHDFLFPAIIAGITSFQVSKALGVPYQYYQIPALPL
                     FSELLFIKTIAIGMICGIAARVFVDMIQAVRTQFLRLRTRFDLWPPLMPLLGGLILAL
                     LILAIPTDYLGLSLPLMDRALHGQAMPYFGFLWKTVLVAVTLGSGFYAGIVTPQFVIG
                     AVTGNAVAHLLGISPMLGAAVGLVAVVAAASNTPLAAIFMGVELFGGVVGTIYVAGAA
                     IAAYLVIGHRSVYPDQRLAYSKTSWLHVRPDMPVGQEKGRLSYGLLRWWQQRR"
     gene            complement(10864..11916)
                     /locus_tag="VFR20_07080"
     CDS             complement(10864..11916)
                     /locus_tag="VFR20_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012365475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEU0230317.1"
                     /translation="MTLQYRIQPHRRLRHLLNPAVRYRHARLLHAVRVALGLLTCVVL
                     TSLLNLSHGEWASITLLVVIGGLQHHGNIRRKAADRGLGTLIGAGVGLVILLAQSTFH
                     HAWLGTLLILIACGICAFHAVGKGGYIALLAAITILIVAGHGDSGIADGLWRAVDVLV
                     GIVIALLFSFALPLYATYSWRHKLADILMQSSQVYAAIAAGNLTGIPEPQRALGTQLV
                     QLRALLPSVTDEIHVSKDQLEEVQHGLRVGISLLEVLAAIRGGIDGKDTQAFLDRYLR
                     DIHRHIQGTLTDMSHALRQGEPRRLEHPEQPAADVDLESVPIRLAPYVRLTQRLSDTF
                     DELRRHLVDTAGRWNI"
     gene            12161..12532
                     /locus_tag="VFR20_07085"
     CDS             12161..12532
                     /locus_tag="VFR20_07085"
                     /inference="COORDINATES: protein motif:HMM:NF025045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HEU0230318.1"
                     /translation="MKNVLGAAMISLSLAAAGLAPLAANAASLTRAQVVEQLQQARAQ
                     GLLSEGESGYPVQQPAASHVSRAAVLTDLALARANGQLNTSESQPYPVDTSAPAHLSR
                     SVVLAQLSAFQEQHPDQFIEH"
     gene            complement(12614..13660)
                     /locus_tag="VFR20_07090"
     CDS             complement(12614..13660)
                     /locus_tag="VFR20_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0230319.1"
                     /translation="MHYRKFGGTGLFVSELCLGTMTFGGQSGIWGEIGQLEQKEATAL
                     VGRSIEAGINLIDTADIYSEGRSEIMTGQALKDLKIPREDVIIATKVFGETGRGANSR
                     GMSRHHIMEGVKASLTRLRTDHIDLYQIHGFDPATSIEEAVRALDNLVRHGHVRYVGV
                     SNWAAWQIMKALGIAERLGLARFESLQAYYTLAGRDLERELAPMLRSEGVGLLVWSPL
                     AGGLLSGKYSRGEQAAAGSRRATFDFPPVNRERAYDCVDVMRRIAQAKDVSVAQIALS
                     WLLHQDVVTSVIIGAKRLDQLNDNIAAIDVSLTADELAELDQVSALPPEYPGWMLARL
                     GQYRYRQLADTTQR"
     gene            13920..14423
                     /locus_tag="VFR20_07095"
     CDS             13920..14423
                     /locus_tag="VFR20_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEU0230320.1"
                     /translation="MAGAMVFSASPARGATLSGGGIGLHLGLGSHYQRLGLAWESPSL
                     WGHAFVGGGSRLDLVGELGVAYWKGTGGHGPDAWQFSAIPFLRWTYHHRFYVEVGVGP
                     TLFTHTTVAGNDISTAFQFGDQIGVGAYLSDNSRLGLRYSHFSNGGIKTPNPGMGVFQ
                     MLYTYQY"
     gene            14701..15417
                     /locus_tag="VFR20_07100"
     CDS             14701..15417
                     /locus_tag="VFR20_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU0230321.1"
                     /translation="MIRPLSVRAISIGLLVVGAFALGITARADTLVEQGRTIATSGSG
                     AAVACVTCHGAKGEGMAAAGFPYLAGQGAGYLALQLHDFASGKRANPIMAPIAKALNA
                     DQVKAVAAYFSHLPAPFDVKSLADRVDTYPPKTDMGAWLANRGDWAHDIPACIQCHGP
                     GGVGVSPHFPAIAGLPAKYIHEQLVAWKTHKRDPGPLALMGDIASRMSDGQMTAVADY
                     FSALPTTPAGAAQSTAGAKK"
     gene            15414..16370
                     /locus_tag="VFR20_07105"
     CDS             15414..16370
                     /locus_tag="VFR20_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU0230322.1"
                     /translation="MKPQFKAWVRGTVMVSALGLASMSAQASGTEPTVPASSAAPAFT
                     PPPADSIPNNAFGKMVREGQDIFLRTGRYAGKYVGNSMNCVSCHIDAGRRAGASPLWA
                     AYVLYPQYRSKNGHVNTLAERLQGCFRFSMNGVMPPADSHTIAALETYMFWMAKGAPT
                     GVKLAGQGFKKLPPPPQPADYVRGQKVFEQHCVLCHGANGEGQGVAGHQVFPALWGDE
                     SYNWGAGMHSVSTAAAFIKANMPLSKGGSLSDQEAWDVAYFVNAHDRPQDPRFNGSVE
                     ETRKKFHGSSADLYGKTINGVQLGAHSTPSGGRLRKTAQAGG"
     gene            complement(16443..16820)
                     /locus_tag="VFR20_07110"
     CDS             complement(16443..16820)
                     /locus_tag="VFR20_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230323.1"
                     /translation="MSDQDQFDRIHAQTSSTETQSGQRPAASLPGGYRQGIITAITVL
                     LGFSLGFLRFWILEAPGKWDTLSVMSAVTLVLSLLGQIAALYRSLLLSDETEREYHKT
                     VQWFIISVFVLIIGLALSIPNSV"
     gene            complement(16836..17309)
                     /locus_tag="VFR20_07115"
     CDS             complement(16836..17309)
                     /locus_tag="VFR20_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEU0230324.1"
                     /translation="MKPDTTDLRILDELQRDGSLSNVELARRVHLSPSPCLARVKSLE
                     AAGIITRYVALANASALGLSLNVFISISLKSQDKESLARFERRIAEHDEVMECYLMTG
                     DSDYLIRVAVADIGALERFILDQLTPIPGIEKIRSSFTLKQVSYKTALPLSARKP"
     gene            17560..20274
                     /gene="aceE"
                     /locus_tag="VFR20_07120"
     CDS             17560..20274
                     /gene="aceE"
                     /locus_tag="VFR20_07120"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEU0230325.1"
                     /translation="MNSPISPDLLAPGGGASHDIDPQETQEWREAFEALTAVHGKRRA
                     SFILDELLAQANRCGLDWRPPIGSAYVNTIPVERQPDFPGDLSLEEKLGAIMRWNALA
                     MVVRANAAYGELGGHIASYASAADLFEVGFNHFFHAGRDGDLVFFQPHSAPGVYARAY
                     LEGRLSENDLAHFRQEILAERDGARGLSSYPHPYLMPDFWQFPTGSMGIGPISSIYQA
                     RFMRYLTHRRLLDCAGRKVWGVFGDGEMDEPESMSALTLAARERLDNLVWVVNCNLQR
                     LDGPVRGNGRIIDELERLFSGAGWHVIKVLWGSDWDGLFAHDTDGALARALAETVDGQ
                     MQTFAAKDGRYNRENFFGRSPALTRLVQGMTDEQIDGLRRGGHDLVKIHAAYAAAAAH
                     RGQPTVILAHTKKGYGMGRAGQGRMTTHSRKKFEDTDLIEFRNRFRLPLTDEQTLGLQ
                     WYKPADDSPEMRYLRERRHDLGGFLPARRTGCEALPVPSPTDYASFALRADGKEMSTT
                     VAFVRLLGALLKDKDLGPRIVPIVADEARTFGMANLFKQVGIYSSQGQRYEPEDIGSI
                     LSYREALDGQILEEGISEAGAISSWVAAATSYSVHGAAMLPFYVYYSMFGFQRIGDLI
                     WAAADQRPRGFLIGATSGRTTLGGEGLQHQDGSSHAHAATVPNCRAYDPAFAGELAVI
                     VDHGMREMLVEQHDAFYYITVTNQNYAQPSLPHGVEDKVVRGCYKYASYEAHSVEPGL
                     HAEADAGATDHAPSVSGVAAPAVARALPRRITLLGSGAILPEVIRAAELLAECGVASD
                     VYSVTSWSELARDGQRCDGDALAGKSVIRPFITSELADSTGPIIAATDYVRMVPESIR
                     AYVPEGRRYLTLGTDGFGRSDTRAELRRHFGVDAESIADAARMMLGLH"
     gene            complement(20332..21003)
                     /gene="alkB"
                     /locus_tag="VFR20_07125"
     CDS             complement(20332..21003)
                     /gene="alkB"
                     /locus_tag="VFR20_07125"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HEU0230326.1"
                     /translation="MAHDLFGHDAGSPLAGTRQRLGLEAIVLRGFALSRAPTLLSAVA
                     AIREAAPCRHMTTPGGYVMSAALTNCGRLGWFSDEHGYRYTPCDPGSGLPWPTMPDCF
                     LALAREAAQAAGFGQYTPDVCLINSYAPGARMSLHQDKDERDHTAPIVSVSLGIPCLF
                     LFGGNQRGDRPARVPLFHGDVVVWGGADRLRYHGVAPIPEATHPLLGNQRINLTFRQT
                     GLEEG"
     gene            complement(21011..21751)
                     /locus_tag="VFR20_07130"
     CDS             complement(21011..21751)
                     /locus_tag="VFR20_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEU0230327.1"
                     /translation="MKDSSAVTPAYETPSRCLGRLNWGFIEQSLNTQGFVVLPDLIPP
                     AQARMMAGLYSDDTLFRARINMESHGFGRGEYKYFRYPIPPEIQDLRTRLYRYLAPIA
                     NRWHAAMGLPARFPLEHTEFIQRCHDAGQMRPTPLLLNYGSDDYNCLHQDLYGEHVFP
                     LQATILLSQPGEDFEGGEFVMTKQMPRRQSQALVAPLGFGDTIVFAVNFLPVPGKRGV
                     TRAALRHGVSRVTAGHRQTLGIIFHDAR"
     gene            complement(21748..22275)
                     /gene="ogt"
                     /locus_tag="VFR20_07135"
     CDS             complement(21748..22275)
                     /gene="ogt"
                     /locus_tag="VFR20_07135"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU0230328.1"
                     /translation="MPTETFFLEHLDTPTGRLCIITDDKGRLRAIDWADHEARMRTLL
                     RRQYKASTVRIEQATRASAARRALLAYFNGDINAIDALPTATGGTEFQRSVWAALRHI
                     PAGQTISYGTLAARIGRPKAVRAVGLANGANPISIVVPCHRVIGANASLTGYGGGIER
                     KRWLLDHEACKAARQ"
     gene            22432..>23828
                     /locus_tag="VFR20_07140"
     CDS             22432..>23828
                     /locus_tag="VFR20_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEU0230329.1"
                     /translation="MNLNPDACYRAVCSRDARFDGRFFTGVKTTGIYCRPICPARTPQ
                     LKNVVFFPTAAAAQEAGFRPCLRCRPEAAPDFAAWRGASDTVSRALAMIELGALDEAD
                     VGALADRLGVGERQLRRLFRQHLGASPVAVAQTRRVLLARQLIQETRLPMTEIAFAAG
                     FGSIRRFNETFLALYGRAAGDLRGTRQPDVPVGSKGEITLLLRYSPPYDWPAVLEFLR
                     QRAIPGVEEVRENLYRRSIQLDGAQGVVIVRPGTADALRVTVRFPKLATLPAIIARIR
                     RVFDLATDPAPIMAHLAGDPILAPVVRARPGLRVPGAWDGFELAMRAVLGQQITVVAA
                     VRLAGKLVAAYGEPLSEPDGALTHAFPQPSALVSVDLAALGMPRSRAAYLSAVAAAVV
                     TDPGLFDATQSLDDAVQKLKGIRGVGEWTAQYIALRQLREPDAFPAADVGLMRAMGTL
                     DGEVWTAAKLLEHAEA"
BASE COUNT         4550 a         7014 c         7538 g         4726 t
ORIGIN      
        1 acgatcaata tgatcaattt tacttatttt tgtgcgtcac ttacacttca ttccgtagtt
       61 tgttgttaac ccttagaacc aggagtttta atgtctttga tcaacacaaa aatcaagcct
      121 ttcaccgcca ccgcgtacca cgacggcaag ttcgtcgatg tgagcgacag cacggtcgcc
      181 ggcaagtggg cggtttttgt tttctatcct gctgatttca cgttcgtgtg cccgaccgag
      241 ctggaagacc tggccaacca ttacgccgaa ttccagaaaa tgggcgtgga ggtctacagt
      301 gtgtcgaccg acacgcattt cgcccacaag gcctggcacg acacgtccga cgcgatcaag
      361 aaggtcagct acccgatgat cggcgacccc acgcacacgc tggcccgcaa tttcgacgtg
      421 ctgatccaag aagaaggcat ttcgctgcgt ggcacgttcg tggtcaaccc ggaagggcag
      481 atcaaggcca tggaaatcca tgacaacggc atcggtcgcg acgcgtccga actgctgcgc
      541 aaggtcaagg ccgcgcagta catcgccgcg catccgaacg aagtctgccc cgccaaatgg
      601 gaagaaggcg cccagacgct gaccccgtcg ctgcatctgg tcggcaagat ctgatgccag
      661 gcccgggcca gcgcccgggt caccaagccc ccgccgtgtc tgtggtctgg cccggcgggg
      721 ctttcacccc gacccttgga accaaagagg cgcctcacga cgcagccttg gggtatgttt
      781 tgccgcctgg ttgcctcgat aggaaaggtt gtctcaccag gaggcaagcc ggtgccatcc
      841 ggtgcggtgt ggggatagtc cccgaattga gagataggtg gagtccatgc ttgacgcaaa
      901 catcaaaagc caactgagca cgtatttcga gcggatccgc ttgccactgg aattgcgcgc
      961 atcgctcgac gacggcggga agtcgagcga actgcgtgcg ttccttgagg aaatcgcagg
     1021 cttgtccgac aagatcacgc tggtggacga acagccacgt gccgcgcgcg tgccgtcatt
     1081 cgccatcgct cgaatcgaca gtgacgtttc cgtgcgcttt gccgggattc cgctgggcca
     1141 cgaactgacg tcgctggtgt tggcgctgct gcaggtgggc ggccatccgg cgaagatcga
     1201 ggatgcggtg gtcgggcaaa tacgcggcct gtcgggtgag taccggttcg aaacctattt
     1261 ttcccagtcg tgccagaact gccccgatgt cgtgcaggca ttgaacgcga tgtcggtgat
     1321 caatcccgcg attcagcacg tggcgatcga tggcgcgttc ttccgcgacg aggtcgaggc
     1381 ccgtgaaatc ctgtccgtgc catccgtttt cctgaacggc gagctgtttc atcagggacg
     1441 cgccggcgtc gaggagcttc tggcgaagct ggacacgggt gatgacacgc gcagggtcga
     1501 tgaattgaat gcccgggatg agttcgacgt gctggtggtg ggcggtggcc cggcgggcgc
     1561 ggcggcggcc gtgtatgcgg cgcgcaaggg tatccgcact gcggtggtgg ccgaacgctt
     1621 cggcgggcag gtgctcgaca ccctggccat cgagaactac ctctcggtgc tggaaaccga
     1681 agggccgaag ttcggtctgg cgctggagca gcatgtcaag gcgtatgaag tcgacatcat
     1741 caacctgcag cgggcggcga agctgattcc cgggaaatcg atacgggtcg agcttgccag
     1801 tggcgcgacg ctgaaggcga aaacggtcat cctgtccacc ggcgcgcgct ggcgcgagat
     1861 caacgtgccg ggcgagcgcg agtaccgcaa tcggggtgtg gcctactgcc cgcactgcga
     1921 tggcccgctg ttcaagggca ggcgcgtggc ggtcatcggc gggggcaact cgggggtgga
     1981 agccgccatc gatctggccg gcatcgtcgg acatgtgacg ttgattgagt tcgaccaggc
     2041 tttgcgcgcc gacgatatcc tgcagcgcaa gctacgcacg ttacccaacg tcgacgtgat
     2101 cacctcggcg cagaccactg gcatcacagg cgacggcaag cgggtcacgg gcctggacta
     2161 ttccagccgc gtggatggct cggtgcatca tgtggatctg gaaggtgtgt tcgtgcaaat
     2221 cggtctggtg ccgaacaccg agtggctgag gggcgcggtg gccctgacac cgcgcggcga
     2281 gatcgaagtc gacgcgcgtg gccagacgtc ggtgccaggt gtgttcgcgg ctggggacgt
     2341 caccaccgtg ccgtacaagc agatcatcat cgcggcagga gacggggcca aggccgcgct
     2401 gggagccttc gatcatctga tccgcagttc ggtcgatgaa gacgaagaaa atgtcccggc
     2461 cgtcgccaat gtggtttgat agcctggcgc atcggcatcg gtgtcggtga cggatggccc
     2521 tcgtgtggaa ttgtcgcgag ggccgttttg ccgcgtatca gaatttcatg cgcatgccga
     2581 caccatacgt gctgccgctc gacaggtcgc tggcgtggtc ggacatgtac gccacataca
     2641 catccgtgcg cttcgacagg ttgtagtcgt agcccagcgc ccaggtgttg cgcttgacgt
     2701 cgctcgcccc cgtgctgcgc gtgaacgcat acgacgccag cacgttgcca ccgcccaggg
     2761 gaacggaaac acccaattgc ccgccattct gcgtgacgtt gccgccgtca atcgtgttgc
     2821 cgatgtactg gtactggccg aatagcttga cgaagctcag gtcgtagctc atgccgacct
     2881 gcaccgcgcg ctgtttgcgg aacccgggga tgacggtgcc caggtctccc ggcgtgttgt
     2941 tgaaattgac ctgctggtat gccacggtcg ccgcgaacgg accatggaag tacagcagat
     3001 tggcgctcca cttgttctgc ccgttctgat cgggttcgtt gccgaaagcg taggcgaagc
     3061 tcgccgtcag tcccttgaaa tcaggcgtcg tgtacatcac ggcatgactc catcctgaat
     3121 cgcccgtgat gccttggccg ttcacgccca ggtaggtgtg gaacaccatg ggtgagaacg
     3181 tgtaggaatc gacgaacgga ttgaacagga tggtggagac gaaatacgag gtggtcaggc
     3241 ggcccatctt cagggtgccc agatcgtcgc tttgcaagcc gacataggcg ttgcgggaaa
     3301 agaacgggtc gccgtcgaag cggccggagc tgcctgtctg gggccggaag aaatcctcca
     3361 tcttgaaaac agccttgagg ccgttgccca ggtcttcgga tcccttgagc ccccagtacg
     3421 aggtcgacat gccgccgcca ccctgtaccc aggtgtgatt gatgtctccg gggtttttct
     3481 gggcaccgac ccaggcatcg atctgcccgt acagctgcac gcttgattct gcgagagccg
     3541 cgccgctggc gcccagtgcg gcaagcaaca cggtgcatgc cagcgcagac tttttgaaag
     3601 ccatttgtct ccatccttgt ttattgtttg tgggttgagc tgcgcccaca agggcgcgcc
     3661 ctttcagtcg attgccgacg aaaggcgagt tttattttga agggaagacc gcgagacttc
     3721 tatcagcgca agttaatggc ctacattcgt gtaggcgtat cgcgcgatgg cttggaatgt
     3781 ggtagggcgg gcattgggtg ttgttcgcct tacgtcacat catcggcgtg ccacatggca
     3841 ggaattgccc ccatcccgaa cgacccgtga gtgacaggtc ccggctcacc accttgccgc
     3901 gggagatcgt gagcaccggc cagcctttga cctcgattcc ctgataaggg gtgtaatcaa
     3961 cgttatggtg cagcatggca ttatcgatga cgacgcgtcg attcggatcc cagagcgcga
     4021 tgtccgcgtc ggccccgacg atgatcgacc ccttgcgcgg atacagtcca tatagcttgg
     4081 cggggttggt ggctgtcagt gcgacaaact ggtggatgtc gatgcggccc tcggcaacgc
     4141 cacctgaaaa cagcaacggc aagcgtgttt ccaggcccgg gataccgttg ggaatatatt
     4201 gaaacggctg ttctttgccg ccaggcattt tcccatacgg gtcgttgtag cggaaagcgg
     4261 catgatcaga tgagaatatc gagaacacat tctgcttcag gccactccag acggcatcct
     4321 gattctgctt gtcccgtggg ggcgggctgc acacgcattt tgcgccatgg tagccctcga
     4381 tatcgagatc ctgttcggtt agaaacacgt actgcggaca ggtttccgca tgaatcggca
     4441 agcctcgtgc ctgagcccag cgtatttgct ctatggcgtc gcggcccgac acatgcacga
     4501 tcaggattgg aacgccggcg agttcggaaa acgtgatggc gcggtgggtc gcttcgcgct
     4561 caaccgccat gggtctcgat gcggcgtgaa actttggggc cgagtggccc tgtgaagtca
     4621 ggcgttccgt cagccacgcg atacagtcgg cgttctcggc gtggatcatc accaaggcct
     4681 ggccggcacg ggccacgtcc agaaccctga gaatgtccag atcgttcagc ttcagatcgt
     4741 cgtaggtcat gtagatcttg aacgacgtgt agccctcttc gataagccgc ggcaactcgt
     4801 ttgcgagcac gtccggggtt ggatcggtca cgatcaagtg gaacgcatag tcgataaccg
     4861 ccttgccgtc tgccctgcgg tggtaatcgt cgacggctgc gcgcaacgac tgccccttgg
     4921 tttgggccgc gaagggaatg attgtcgtgg tgcccccgca cgcggcggaa ttcgttccgg
     4981 tcacgaaatc gtccgccatg cgcacgcctt ccggcatggg ctgatcaagg tggcagtgtg
     5041 cgtcgattcc cccgggaagg gtaagcaacc ccgtggcatc gatggtttcc gacgcgttgc
     5101 ccaacccgtt tccaagcgcg gcaatcttgc catcctggat tccaatatca caattgaatc
     5161 ggtcggaagc cgtcaccaca tcggcattgc gcagaatcag gtcgaattca gcgttcatgt
     5221 tcatcacgcc ttgtgcattg gttcgcggaa cagactgccc cagccaatat caccatgtat
     5281 ctcgtagccg gcgagagaca gggatttcca caccaccgtg gaaatcgtat cgtagacagg
     5341 aatccccgta agggcctcga gctcgggtgc tagatgcgcg gccctcagat tggtgcagaa
     5401 agtcgtgatg gcctgaggcc gctgttcggc aacctgcatc accatcgtac gcacctgctc
     5461 gtccgtgacc tcggaaaact cgaaattgat gtggagatca aggtgccgtt cggcgacaca
     5521 gttgacgccg gcgtccaggt agttggcgat gattttttcc tggacggccg ccagataagg
     5581 cgaaaccagc gcgaaatccg acactttcct gcgttcgaag atttcattca gcgccagcac
     5641 cgatgttgtg gccggaatgc cagtggcctg ggtgatgcgt tcacacagac gctgatcccg
     5701 ctcgaacccc agccagcccg acgatgtgcc gttccaggcg atcacgtcca ccttggcgtc
     5761 agccagcgat cgggcggcat ccagcatccg ggtttcgtcg aactgctgtt gtgaacgctc
     5821 tccgagggat atctcggtaa ccggaaatcg tgcaaagtgc gccgatacgc ctggcaaagc
     5881 cgacacgatg gcgctggtga caggttcgag agtggtgttt gaagaagggg tcaacatccc
     5941 cagtagcgtg cgttttgtca tgacatttgt ttgttcattt gtcttcatta tggaatatcg
     6001 ccttgaagtc cggtgtcgaa ggctccggag cggcgagatc cagcgtctgc tctatgtggt
     6061 tgaggtgatc gagcatgcac tgacgggcgc cattgctgtc gcgtgtctcg atcaggctga
     6121 ccagttcccc atgttcgtga tgggcgcagg ccggcgtacc cggtttgtcg tatagcgtga
     6181 tgatcaggca ggtcagcgcg gtaagttccc gcatgatgcg tggcaagaaa ctcttcgccg
     6241 ccatgtccgc caaatggata tggaattccc ccgataaccg gatgatgcga tgctggtctt
     6301 tcgcgtcgcg ggcttcctgt tccagcgcga cactgcggcg cagggtctgg atctggctct
     6361 tggtggcgtt ttcagcaagc aaagccacca tgccgggctc gagcagcctg cgtgtgatga
     6421 aaagttcgcg ggcctcttgc accgtcgggc gggaaataaa ggcgccacga ttgggaatca
     6481 gtgtcacgac tttctcatgt gccagacggc ccagcacctg ccgcactttg gcgcggcttg
     6541 cgccggtggc ctcggtcagg cgttcctcgg ccagtttcgt tcccggagcg agcctgtgtt
     6601 caagtatggc gatctggatt cgctcgtaaa tctgatcgtt cgacatgcca tgtgtagccg
     6661 atacatctat gctgccttgt gataccagtg ttgccgcgac gtccacgtta tgatccttga
     6721 atagcctggg agagtgtgcg atcggctccg ttcaagcaga gttttatgca cacattatcg
     6781 cgcttgattg tatacattcg aaatccgaat cagtgcctta ctaaaaccgc ttggagaaag
     6841 cgccaggcct atcgacaaaa acctggctat gctaaggtgc cctgtgcaag caatttccgc
     6901 atggcgtccg acaagccctt gttgggtttg atcggcggca gtgaaaagct gccgccccgg
     6961 gattcgccgg gagcaagggc gatgagtgtc tcggcatggc ggaccgcgct cgatacgcca
     7021 tcgacaaccg gcaccggaat gtcaccccgg atcgtgcgcg ccagtccggc aagcggcgcg
     7081 cccgccagga tcaacacatc ggctccgtct tcgcgcaccg cctgatcgca gagatcccgc
     7141 aagcgccctt cgtgatccga ctgtacggaa ccgatatctt ccagaggctg ccgcaggcat
     7201 cgaatgctgg ccaatcggtc cagcagaccg ttggcctgca cacattcgcg ataccaggcg
     7261 gtgatgcgat gtgaaatggc gatgatggaa tacctattgc ccagcatgca ggcactcatc
     7321 aaggcggcct ccgtaagccc gaccaccggt atgtcgacga gttcacgtat cccccataga
     7381 ccgggatcgc cgaacgccgc gatcaccagc gcgtcatagt tcggcgcctg ggtggccgcg
     7441 aggtccgccg cggcataggc gcccagaagt gcctccagcc gagtctcgat gtaagcaacg
     7501 ccaagcgggg cggtaaccac atcgatatgg gtcgaggccg aggccgttct acgggcctcg
     7561 gcagcgatca gatccgagac gctttcggaa atattcggat tgatcaacag aatcttgctc
     7621 atgtcagtca ccgtcttggg tcccgtttta tttatatggg cagctttttg tcgtaatcga
     7681 taagcagggt ggaacaaaca aacagcccca ccccagccgc cgcgaacaac atgaatgccc
     7741 agaagaagga tccagtcgcc tgcacgatca ggccgaccag gattggcacg cccaccccgg
     7801 cgatgttgcc accgagattc atcgtgcctc cgaggaagcc gacactattg cgggtgccaa
     7861 ggatggacgg aacacaccag tacaagccgc accagcgcag gaagaacagc gtgaccgaga
     7921 gtgtgaccac caccatgatt ggatcggaga cctgtgtgac actgaaaatc gagaccgtgg
     7981 cgagaatcgc cgccacgccg cagagcgtgc gcatcacacg gccttgagag cctcccgcct
     8041 cccgccactt gtcggccaac caaccaccca cgagttctcc cacgaaacct gcgaagaaca
     8101 tgatgaacac agcgccgccc atttgcttga tgttcagccc gcgcgcctcg gacaagtagc
     8161 tgggcaccca ggtcagcaat ccatagaaca aagtgttgaa gaacatccaa ccgaagaaca
     8221 tgccccacac cgagcgatac ttgaagaagt cgagcttgcg tccgctgaca accttgggtt
     8281 cggccgcaag ttcgcgttcg tggctgattt cgatgtggcg ggcttccgcg tcgttgaccc
     8341 cgggatgctg ccgtgggtgg ttgcggatgt agtgccatga ccatagtcca gcaatcatgg
     8401 tgccaacgcc cgcaatgatg aacgcagtgc gccacgagtc gaacatagcg atcaggaacg
     8461 agatgagaat ggcgcccagc gcagacccca gcggagcccc gccatcaaga agcgtggcac
     8521 ctcggccgcg ctcgttctgg gacatccaga tcgagttgag cttgccaccg gctggataaa
     8581 tgggggcttc cgatgcaccc aggccaaggc gcgtgaggag tagtccgaac caccccgtgg
     8641 atatagcggc caacgcctgg aaaagtcccc agaaaatggt cgccgaggaa atcaccacgc
     8701 gcgggccatg ccgatcggcc agcatgccgc ccggaatctg catcagcgcg tatgtccaga
     8761 agaacgcact catcacaagc ccctggaagg ccgggccgat gttgaattcc ttgccaatca
     8821 acggcatggc aaccgaaatg gaggcgcgat caacgtaatt gatcgtcacc agaaaaagca
     8881 tgaggaagaa tatcttccag cgcacgcggg tttgagggac ggcgaattcc gccgccacgg
     8941 cttgaggttg ggcgcccatg gtttgtctcc tgtatccagt ttttgtcgat gactggttct
     9001 attgatgaaa aaagcggtgg aaactctgaa aaagaatggc aggcttctta tatcgctttg
     9061 cattattgtc aatcatcaca tatttttttg tcaattaaat ttattatgat tgttaacaat
     9121 aattattgtg agcgtaaccc accatgggtg ttaaccgttc tgattgcgaa atgtgcactc
     9181 tgtgacgccc agcctggccc ggcccgccac ggccctggct tggtggcgag tgtccgatcg
     9241 agttgggatc tggaaagtaa gacggagggc agtggagcac agcgtccgcg cacgccattt
     9301 cccacttgtg ggttgaggcg cttgggttta cgttggctct gcacgccgcc aatgtttcaa
     9361 aaaggccttg ggtgcatcac cgccgttgct gccaccatcg cagcaaccca taggacaggc
     9421 ggcctttttc ctggcccacc ggcatatcgg gccgcacgtg cagccaggat gtcttggagt
     9481 aagcgaggcg ttggtcggga tagacgctgc gatggccgat gaccaggtag gcagcgatgg
     9541 cggcgccggc cacgtaaatg gtgccaacca cgccgccgaa caattcgacc cccatgaata
     9601 tggcggccag cggcgtgttc gatgcggcgg cgaccaccgc caccagaccc accgccgcgc
     9661 cgagcatggg gctgatgccc agcagatgcg ccacggcgtt gcctgtgacc gcgccgatca
     9721 cgaattgcgg tgtgacgatt ccggcgtaaa agccggagcc cagcgtgacc gccaccagca
     9781 ccgtcttcca taggaatcca aagtacggca tggcctgccc atgcagggcg cgatccatga
     9841 gcggcaggct caggcccagg tagtcagtgg gaatggccag gatcagcaag gccaggatca
     9901 ggccgcccaa cagcggcatg agtggtggcc agagatcgaa gcgggtacgc agacgcagga
     9961 actgagttcg aacagcctgg atcatgtcca cgaagacgcg cgccgcgatg ccgcagatca
    10021 tgccgatggc gatggtcttg atgaaaagca gttccgagaa tagcggcaag gcggggatct
    10081 ggtagtactg gtacgggacg cccaacgctt tgctgacctg gaatgaggtg atgccggcga
    10141 tgatggccgg gaacaggaaa tcgtgccgaa tgcggccgat ggacagtacc tccacaccat
    10201 atatcgcgcc cgcgatcggt gtgccgaaca cgctggcgaa gcccgcgctg acgccacagg
    10261 ccaccaggcg gcgttgcagt tcgggattca gtttaagaag ctggccgatg ccggccgcca
    10321 acgaggcacc gatatgcgag caaggacctt ccttgccggc agacccgccg caggccaggg
    10381 tgatgatggc ggccacgggt ttgatcgccg ctgtcttgaa tggcattttg ccattctggt
    10441 cgtggacagc ggcaatggcc gagtctttca ggccagtggt attggccttg tatccgtagt
    10501 acagcagcaa cccattcagc aacccgccgg tgggcagcag caacatctgg aaccagagcg
    10561 gtatgttggc ggatttgccg gtcaggtaga acagggcatg caggaaaacg ctggtgccgc
    10621 agcccacgat ggccccggtg atcgaggcaa ggaccagcca ctgcacgacc gtggcaagca
    10681 tgagcaggaa ttcgacaaca tcagagcgcc gcatggcagg tgtctccggg ggcccgatcg
    10741 ggcacggtgg ggtaggcggg gtttggggag cctgtcatta tatgagccag cgcacccccg
    10801 ccggatgcga tatggcgcgc ttcgcgccgg ttcgaggcgt ttgtggtgcg tggtggccgt
    10861 cgctcagatg ttccaccgcc ccgcggtgtc gacgagatgc cgtctcagtt cgtcgaatgt
    10921 gtccgagagg cgttgcgtca gtctcacgta cggtgccagc ctgatcggca cggattcaag
    10981 atcgacgtcg gccgcgggtt gttcagggtg ttccaggcgc cgcggctcgc cttgcctgag
    11041 ggcgtgtgac atgtcggtca acgtgccttg aatgtggcga tgaatgtcgc gcaaatacct
    11101 gtccagaaac gcctgggtgt ctttgccgtc gataccgccc cgaatcgcgg cgagaacctc
    11161 gagcaggctg atgcccacgc gcagcccgtg ctgcacctct tcgagctggt ctttggagac
    11221 atggatctca tccgtgaccg atggcagcaa cgcgcgcaac tggacaagct gtgtgcccaa
    11281 ggcgcgttgg ggctctggta ttcccgtcaa atttcctgcg gcgatggcgg cgtatacctg
    11341 tgagctttgc atcaggatgt ccgccagctt gtggcgccag gaatacgtgg catacaaggg
    11401 cagggcaaac gaaaagagca atgcaatgac aatgccgacc agcacgtcga cggcgcgcca
    11461 aagcccgtcg gcgatcccgc tgtcgccatg gcccgcgacg atcaggatgg tgatggcggc
    11521 gagcagggcg atatagccgc ccttgcccac ggcgtgaaag gcgcagattc cacaggcgat
    11581 caggatcagc agagtgccga gccacgcgtg atgaaaggtt gactgtgcca gcagaatgac
    11641 gaggccgact cccgcgccga tcagtgtgcc cagcccgcgg tccgccgcct ttcggcggat
    11701 attgccatgg tgctgcaatc cacctatgac caccagcagt gtgatggacg cccattcgcc
    11761 atgagacagg ttcagcagac tggtgagtac cacacaagtc agcaacccca gggcgacccg
    11821 caccgcgtgc agcagtcggg cgtggcggta gcggaccgct ggattcagca ggtggcgcag
    11881 gcggcggtgt ggctgaatcc ggtattgcag tgtcatgtgg gatttatttt ttcaaaaacg
    11941 atattagttt ttttaaatca ctgacggtat gcgtttgatt tggtaggata ttcgctgact
    12001 ttcaaaacat tatttccatt tttggaaata tcttttcaat gaataagggt ttataccagg
    12061 ttcgtcaagg aggatactca cttcatcgat tgtgagcgcg ctcaagcaat ttcgccagtc
    12121 cgcctcctcg cgtcgcatta ttgtattgat ggagtttgtg atgaaaaacg tacttggcgc
    12181 cgccatgatt tccctttccc tcgccgccgc cggtctggcc ccgcttgccg ccaacgccgc
    12241 gtcgttgact cgcgcccagg tggtcgaaca actgcagcag gcgcgtgcac agggtctgtt
    12301 gagtgaaggg gagtctggct accccgtgca acaacccgct gccagccatg tgagccgcgc
    12361 cgctgtgctg accgacttgg cgttggcgcg cgccaatggc caactcaaca ccagcgaatc
    12421 gcagccgtat ccggttgaca ccagcgcgcc agcccatctg tcgcgtagcg tggtgctggc
    12481 acagttgtcg gcgttccagg agcagcatcc cgatcagttc atcgaacact gatcgcgtcc
    12541 gaccgggatg gtgggtgctg tctcggaatg gtgtgcttct cgcgccggag cggtgtatgc
    12601 cgctccggcg ttgtcatctt tgggtggtgt ccgcgagttg ccgatatctg tactgcccca
    12661 ggcgcgcaag catccagccc gggtattcgg gaggcagcgc actgacttga tccaattcgg
    12721 ccaattcatc ggcggtcagc gagacgtcga tcgcggcgat attgtcgttg agttgatcga
    12781 ggcgcttcgc gccgatgatg acgctcgtga cgacgtcttg atgcagcaac caggacagcg
    12841 cgatttgcgc cacagacacg tccttggcct gggcgatgcg gcgcatgacg tccacgcagt
    12901 cgtaggcgcg ctcgcggttg acgggcggga agtcgaacgt ggcgcggcgg gaacctgccg
    12961 cggcctgctc cccgcgacta tatttgccgc tgagcaggcc gccggccaac ggactccaga
    13021 ccagcagccc gaccccttcg ctgcgcagca tcggcgcgag ctcgcgttcg aggtcgcgcc
    13081 ccgcgagcgt gtagtaggct tgcagcgatt cgaatcgggc cagtccgagc cgttcggcaa
    13141 tgcccaacgc cttcatgatc tgccaggccg cccagttcga aacgccgaca tagcgcacgt
    13201 ggccgtgccg gaccaggttg tcgagcgccc tgaccgcttc ttcgatggat gtggcaggat
    13261 cgaacccgtg gatctggtag agatcgatat ggtcggtccg caggcgcgtc aggctggcct
    13321 tgacgccttc catgatgtga tggcgcgaca tcccccggga attcgcgccc cgaccggttt
    13381 caccgaagac cttcgtggcg atgatcacgt cttcgcgtgg gatcttcagg tctttcagcg
    13441 cctggccggt catgatttca gaccggcctt ccgagtagat gtcagcggtg tcgatgaggt
    13501 tgatgccagc ctcgatggaa cgccccacca atgcggtggc ttctttctgt tccaattggc
    13561 ctatttcgcc ccatatgccg ctttgtccgc cgaacgtcat ggtgcccagg caaagctccg
    13621 acacgaaaag gccggtgccg ccaaatttcc gatagtgcat tgtgtctact ccgtacataa
    13681 ggtgtcgcca tgtggaatta tggactgatt gtcttgtgcg ggccatgttg ttacagccac
    13741 gtcatttgcc ggatgcgtcc tgtgcatatc gcttatcatc ctcccgttct gcctgggaag
    13801 gcttgtgccg caagccttcg cacaacctgt cggaattccg gcatatccac cgacgattca
    13861 tgaaaggtct tcacatgggt gcggtcaacc gattgtcgtc aattgcagca atattcgcca
    13921 tggctggcgc catggtgttt tcagcttctc ccgccagggg agcgaccttg tccggcggcg
    13981 gtattggcct tcacctgggg ctgggcagtc attatcaacg tctcgggttg gcctgggaat
    14041 ccccgtcgtt gtgggggcat gcgtttgtcg gtggcggcag ccgcctcgat ctggttggcg
    14101 aactgggtgt tgcctattgg aagggtactg gtggccatgg cccggatgcc tggcagttca
    14161 gtgccatccc gttcttgcgc tggacctacc accatcgctt ctatgtcgag gtgggagtcg
    14221 gccccacgct attcacgcat accaccgtcg cgggaaatga catcagcacc gcgtttcagt
    14281 tcggcgatca gattggggtg ggtgcctacc tgtcggacaa cagccgcttg ggcctgcgtt
    14341 attcgcattt ttcaaacgga ggcatcaaga ctccgaaccc gggcatgggc gttttccaga
    14401 tgttgtacac ataccagtat tgaccggcgc gttgtcgata cgtcttgcct gacaagcccg
    14461 tgcatccgaa tttgaacccg cgaatccacc acacggcaca ctgctgcgct ggttcgtaat
    14521 tttcatttcg aaatatttgg ttcggcaacg ctaccttaca atatggaatt ggtgtttcgt
    14581 tcatcaagca gccgcgctca agttggaaag caagggtcgg ctgagcgaaa cactggcggg
    14641 ctggcccgaa cggcgcgaga caagcggccc tgggcttgac ctctcacggg agcggaaatc
    14701 atgatcagac ccctatcagt ccgtgcgatc tcgatcggcc tcttggtcgt cggggctttc
    14761 gcattgggta tcacggcgcg ggccgacaca ctcgtcgagc aaggtagaac catcgccaca
    14821 tcgggcagcg gcgcagccgt tgcctgtgtc acctgccacg gtgccaaagg cgagggcatg
    14881 gcggcggctg ggtttccgta cctcgcgggg caaggcgccg gttatctagc cctgcaactg
    14941 catgatttcg cttcgggaaa aagggcgaac cccatcatgg cgccgatagc caaggcgctg
    15001 aatgccgatc aggtcaaggc cgttgccgcg tatttttccc atttacccgc gcccttcgac
    15061 gtcaagtcgc tggccgatcg ggtcgatacc tatcctccaa aaacagacat gggtgcctgg
    15121 ctggcgaatc gtggcgactg ggcgcatgac atccccgcct gtatccagtg ccatgggccg
    15181 ggcggggttg gcgtgtcacc gcatttcccc gccatcgccg gcctgccggc gaagtacatt
    15241 catgaacaat tggtggcgtg gaagacgcac aagcgtgatc cggggccgct ggcgctgatg
    15301 ggcgatatcg ccagtcgcat gagcgatggc caaatgaccg ctgtcgccga ctatttctcg
    15361 gcgctgccca ccaccccggc cggagcggcc caatccacgg cgggagctaa gaaatgaaac
    15421 cgcaattcaa agcatgggtg cgcggcacag tcatggtttc cgcgctcggg ttggcgtcga
    15481 tgtcggcgca ggcgtcgggt accgaaccga ctgtgccggc atcatccgcc gcgcccgcat
    15541 tcacgccccc gccggcagac tcgattccca acaatgcgtt tggaaaaatg gtgcgcgagg
    15601 ggcaggacat ctttctgcgg accggccggt acgcgggcaa gtatgtgggc aactccatga
    15661 actgtgtcag ttgccatatc gatgcggggc gccgcgcggg ggcgtcgccg ctgtgggccg
    15721 cctacgtgct gtatccgcaa taccggtcga agaacggcca cgtcaacacc ttggcggagc
    15781 ggctccaggg ctgtttccgc ttcagcatga acggtgtcat gcccccggcc gacagccaca
    15841 ccatcgcggc gctggaaacc tacatgttct ggatggcgaa gggggcgccc actggcgtga
    15901 agctggccgg ccagggtttc aagaaactac cgccgccccc ccagcccgcc gactacgtgc
    15961 gcggccagaa ggtgttcgag cagcattgcg tgttgtgtca tggcgccaac ggcgaaggac
    16021 agggtgtcgc cggccatcag gtgtttcccg cgctgtgggg cgatgaatcc tacaactggg
    16081 gggccggcat gcacagtgtg tcgacggccg cggcgttcat caaggccaac atgcctttga
    16141 gcaaaggtgg ctccctgtcg gatcaggagg cttgggatgt ggcgtatttc gtcaacgctc
    16201 acgacaggcc gcaagacccc cggttcaacg gttcggtgga ggaaacccgt aagaaattcc
    16261 acggttcgtc cgccgatctg tacggcaaga cgatcaatgg cgttcagctt ggggcgcact
    16321 caacgccatc gggtgggcgc ttgcgcaaga ccgcgcaagc gggcggctag cccataaggg
    16381 gagcgccgat cgattcatgg cgcttgtctt ccgcttgacc aggtcaacgg ctatcagtgc
    16441 ggtcacaccg aattcggaat cgaaagggcc agaccgatga tcagcacgaa gaccgatatg
    16501 atgaaccatt gcacagtctt gtgatactcg cgttcggttt catcggaaag caataatgac
    16561 cgatacaggg ccgcgatctg ccccaatagg gacaacacca gcgtcacggc cgacatgacg
    16621 gacaaggtgt cccatttgcc tggggcttcg agaatccaga atcgaaggaa acccagcgag
    16681 aaaccgagca agacggtgat ggccgtgatg atgccttgcc gatagcctcc tggtagcgat
    16741 gccgctggtc gctggccgga ttgtgtctcg gtcgagctgg tttgggcgtg gatgcgatcg
    16801 aactgatcct ggtcggacat ggtggtattt ccgccttagg gcttccgcgc tgaaagtggt
    16861 agcgcggtct tgtatgaaac ctgcttgagc gtgaagctgg agcgaatctt ctctatcccc
    16921 ggtattggtg tgagctggtc caggatgaac cgttccagag cgcctatgtc cgccaccgcc
    16981 acccggatca gataatcgga atcgccggtc ataaggtagc actccatgac ctcgtcgtgt
    17041 tcggcgatgc gccgctcgaa ccgcgccagc gattccttgt cctgggactt caggctgatg
    17101 gatatgaaca cgttcaggct caagcccagt gcggaggcgt tggccagcgc aacgtagcgg
    17161 gtgatgatgc cggccgcctc gagcgacttg acgcgcgcca ggcaggggga aggcgacaga
    17221 tgcacgcgcc gagcgagctc gacgttgctc aacgagccgt cgcgctgcaa ttcgtcgaga
    17281 atgcggagat cggtggtgtc cggtttcatg tgctgtgatt tgcgtagttt caataatttt
    17341 atgctgaata tctaattatt ttcgcgatat ttggcatcta atttgccgcc ttgaggttca
    17401 aaattcagga accttctgaa caagccaccg cgccgcgcat gtctgcggcc tgatccgggg
    17461 atccttggcg tgcgcaggca tcagaatcgc gccccaccca ggcaaagccg tgcgtgggca
    17521 agccgcccca ccgttgggct gttgttgtcg aggtatacga tgaactcccc tatttccccc
    17581 gatttgctgg cgcctggcgg cggagcgagc cacgatatcg atccacagga aacccaggaa
    17641 tggcgtgagg cgtttgaagc gctcaccgcc gtgcatggca agcggcgtgc atcgttcatc
    17701 ctggatgaat tgctggccca ggccaatcgt tgcggcttgg actggcggcc cccgatcggt
    17761 tcggcttatg tcaacaccat tcccgtcgag cgccaaccgg atttccccgg cgacttgtcc
    17821 ctggaagaga aactgggcgc catcatgcgc tggaatgccc tggccatggt ggtgcgcgcc
    17881 aatgccgcct acggcgaact gggtggccac atcgccagtt acgccagcgc ggccgacttg
    17941 ttcgaggtcg ggttcaacca cttcttccac gctggtcgcg atggggatct ggtgttcttc
    18001 cagccgcaca gcgcacccgg cgtgtatgcc cgtgcctatc ttgaggggcg gttgagcgag
    18061 aacgatctgg cgcatttccg ccaggagatc cttgcggagc gcgacggtgc gcgtggcctg
    18121 tcgagctacc cgcatcccta tctgatgccg gatttctggc agtttccgac cggatccatg
    18181 ggcatagggc cgatcagttc catttaccag gcgcgcttca tgcgctatct cacgcatcgt
    18241 cgtttgcttg attgcgccgg ccggaaagtc tggggcgtct tcggtgatgg ggaaatggac
    18301 gagcccgaat ccatgagcgc gttgacgctg gcggcgcgcg agcgcctgga caatctcgtg
    18361 tgggtcgtga attgcaatct gcagcgcctg gatgggccgg tgcgaggcaa cgggcgtatc
    18421 atcgacgagc tcgaaaggtt gttctcgggc gcgggctggc atgtcataaa agtgttatgg
    18481 ggcagcgact gggacggcct gttcgcgcat gacacggatg gggctctggc ccgggcgctt
    18541 gccgagacgg tcgatggtca gatgcagacc ttcgccgcca aggacgggcg ctacaaccgc
    18601 gagaacttct ttggccggag tccggcgctc acgcgtctgg tgcaaggcat gaccgatgaa
    18661 cagatcgatg gcctgcgtcg cggcggccac gacctggtga agatccatgc cgcctacgcc
    18721 gcagcggcgg cgcatcgtgg ccaacccacg gtgatcctgg cccacaccaa gaaaggctat
    18781 ggcatggggc gcgccggcca aggccgcatg accacgcact cgcgcaagaa attcgaagac
    18841 accgatctga tcgaattccg caatcgcttc aggctgccgc tcacggatga gcaaacgttg
    18901 gggctgcagt ggtacaagcc cgccgacgac agtccggaga tgcgctatct gcgcgagcgg
    18961 cgacatgact tgggcggctt cctgcctgca cgacggacag ggtgcgaggc cctgcctgtg
    19021 ccgtctccca cggattacgc gtcgttcgcg ctgcgcgccg acggcaagga aatgtcgacc
    19081 accgtcgcgt tcgtgcgttt gctgggggcg ctgctgaagg acaaggacct tggcccgcgc
    19141 atcgtgccca tcgtggccga tgaggcgcgc acgttcggca tggcgaatct gttcaagcaa
    19201 gtggggatct attccagcca gggccagcgt tatgagcccg aggacattgg ttccatcctc
    19261 agctatcgcg aggcgctgga tggccagatc ctggaagaag gcatcagcga ggcgggggcg
    19321 atttccagct gggtcgccgc ggcaaccagc tacagcgtgc atggcgcggc catgctgccg
    19381 ttctatgtct actattccat gttcgggttc caacgcatcg gcgacctgat ctgggcggcc
    19441 gccgatcagc ggccacgcgg cttcctgatc ggcgccacgt cgggccgcac cacgctgggc
    19501 ggtgaaggcc tgcaacacca ggacggcagc agccacgcac acgccgccac cgtccccaat
    19561 tgccgcgcgt acgatcccgc gttcgctggt gaactggcgg tcatcgtcga ccacggcatg
    19621 cgcgagatgc tcgtcgagca gcatgacgcg ttctactaca tcaccgtcac gaaccaaaac
    19681 tacgcgcagc ccagtctgcc acacggagtg gaggacaagg tcgtgcgcgg gtgctacaaa
    19741 tatgcatcgt acgaggcgca tagcgtggaa cctggccttc acgccgaagc ggatgctggc
    19801 gcgactgacc atgcgccgag cgtttccggt gtggctgcgc ccgccgtcgc gcgcgcgttg
    19861 ccgcgacgga tcaccttgct gggatcgggc gccatcctgc ccgaggtcat ccgcgcggcg
    19921 gagctgttgg ccgaatgcgg cgtggccagc gacgtctaca gtgtcacaag ctggagcgag
    19981 cttgcccgcg acggccagcg ttgtgatggt gacgccctgg ctggcaaatc cgtgatccga
    20041 ccgttcatca cgagcgagct ggcggactcg actgggccca tcatcgcggc aaccgactat
    20101 gtgcgcatgg tgcccgagtc tatccgagcc tacgtgccgg aggggcggcg ctacctgacg
    20161 cttgggactg acggttttgg gcgcagcgac acccgtgccg aactgcgccg gcatttcggg
    20221 gtggacgcgg aaagcatcgc cgacgccgcg cgaatgatgc ttgggttgca ctgagccggc
    20281 acctgcgcat gatcgcgagc accgcatacc gccagcccat ctgggctggc gtcagccttc
    20341 ttcaagcccc gtctgccgga acgtcagatt gatgcgttga tttcccagca gcgggtgggt
    20401 ggcttccgga atcggggcca cgccgtggta gcgcaggcga tcggcaccgc cccagaccac
    20461 cacgtcaccg tggaacagtg gcacccgcgc cgggcggtcg ccgcgctggt tgccgccaaa
    20521 caggaacagg catgggatgc caagggacac cgacacgatg ggcgctgtgt gatcgcgttc
    20581 atccttgtcc tggtgcagcg acatgcgcgc acccggcgcg taggagttga tcaggcagac
    20641 atcgggcgtg tattgcccga acccagcggc ctgtgccgcc tcgcgagcca gggccaggaa
    20701 gcagtcgggc atggttggcc atggcagccc ggagcctgga tcacagggcg tgtagcgata
    20761 gccgtgttca tcactgaacc atccgaggcg gccacagttc gtcagcgcgg ccgacatgac
    20821 atagccgccc ggtgtggtca tgtgacggca gggcgccgcc tcccgtatgg cggccacggc
    20881 ggaaagcagc gtcggcgcgc gcgacagggc gaaaccgcgt aacacgatgg cttccagccc
    20941 gaggcgctgc cgtgtgccgg ccaaagggga ccccgcgtcg tgtccgaaca gatcgtgggc
    21001 catggaggga tcagcgcgcg tcatggaaga tgatgcccag cgtctggcga tggccggccg
    21061 tcacccggct gacgccatgc cgcaaggcgg cacgcgtgac gccgcgcttg cctgggacag
    21121 gaaggaaatt gacagcgaac acgatggtgt cgccaaagcc aagaggcgcg acgagtgcct
    21181 gggattgacg acgaggcatc tgcttggtca tgacgaactc gccgccttcg aagtcttccc
    21241 cgggttgtga tagcaggatg gtggcttgca ggggaaacac atgctcgccg tacagatcct
    21301 ggtgcaggca gttgtagtcg tcagaaccgt agttgagcag caatggcgtg ggacgcatct
    21361 ggccggcatc gtggcaacgc tgtatgaatt ctgtgtgttc gagcgggaaa cgcgctggca
    21421 gccccatggc cgcatgccag cggttggcga tgggggccag atagcgatac agccgcgtgc
    21481 ggagatcctg gatctcggga ggtatcgggt atctgaaata cttgtattcc ccgcggccga
    21541 atccgtggct ctccatgttg atgcgggcac ggaaaagcgt gtcgtcggag tacagaccgg
    21601 ccatcatccg tgcctgtgcc ggaggaatca gatcaggcag aaccacgaac ccctgtgtat
    21661 tcaaggactg ctcgatgaac ccccaattca ggcggcccag gcagcggctg ggtgtttcgt
    21721 acgccggggt caccgcggag ctgtctttca ttgtcgggcc gccttgcaag cctcatggtc
    21781 gagcagccag cgcttgcgct ctatgccgcc gccatagcct gtgagcgacg cgttcgcgcc
    21841 aatgacccga tggcacggga cgacgatgga gatggggttc gcgccattgg ccaaccccac
    21901 cgcccgcaca gccttgggcc ggccaatgcg ggcggccagg gtgccgtagc tgatggtttg
    21961 tccggcgggg atgtggcgca gggcggccca taccgaacgc tggaattccg tgccaccggt
    22021 ggcggtggga agcgcgtcga tggcgttgat gtcgccattg aaataagcca acaacgcccg
    22081 ccgtgccgcg gaggcgcggg ttgcctgctc gatacggaca gtactggcct tgtattggcg
    22141 gcgcagcagt gtccgcatgc gtgcttcatg gtcggcccag tctatggcgc gcaggcggcc
    22201 cttgtcgtcg gtgatgatgc aaagacgccc ggtaggagtg tcgaggtgtt ccaggaagaa
    22261 ggtttcggtg ggcatgatga ggactccgga ttgagtgttc acggtctcac tgtaatggtc
    22321 tcgcaagctg gttgctagcg gttttcagac ggcaacgtgg tttgaggaaa ttctgatgtt
    22381 gacgtccgaa aaccgctaga tattgacgcc atccagtcgt accatgtctt catgaacttg
    22441 aaccctgatg cctgctaccg ggcagtgtgc tcgcgtgacg cgcgcttcga cggccggttc
    22501 ttcaccggcg tcaagaccac gggtatttat tgtcgcccca tttgcccggc gcgcacgccc
    22561 caattgaaga acgtggtgtt cttccccacg gcggccgccg cgcaggaggc cggattccgc
    22621 ccctgtctgc gctgccggcc tgaggccgcg ccggacttcg ccgcatggcg gggagcgtcc
    22681 gacaccgtgt cgcgtgccct ggccatgata gaactgggcg cactggatga ggccgatgtc
    22741 ggtgcgctgg ccgatcggct gggggtgggg gagcgtcaac tgaggcgcct tttccgacag
    22801 cacctgggcg cgtcgcccgt tgccgtggcc cagacccgcc gtgtcttgct ggccaggcag
    22861 ctcatccagg aaacccgcct gcccatgacg gaaatcgctt tcgcggcggg gttcggcagt
    22921 atccgccgct tcaacgaaac gtttctcgcg ctgtacggtc gcgcggcggg ggatttacgt
    22981 ggcacccgcc aacccgatgt cccggttgga tcgaaggggg aaatcacgtt gctgctgcgt
    23041 tatagcccgc catacgattg gcccgctgtg ctggaattcc tgcgccagcg tgccattccc
    23101 ggcgtagagg aagttcggga aaatctttat cggcgatcga ttcagctcga tggcgcccag
    23161 ggcgttgtca tcgtgcggcc cgggaccgcc gatgcattgc gtgtgacggt gcgctttccc
    23221 aaacttgcca cgttgccggc catcatcgca cgcattcggc gtgtgtttga tctggccacg
    23281 gatcccgcac ccatcatggc gcacctggca ggtgatccga ttctcgcgcc ggtcgtcagg
    23341 gccaggcctg gtttgcgtgt gccgggcgct tgggacggct tcgagctggc catgcgcgcg
    23401 gtgttggggc aacagatcac cgttgtcgcc gccgttcgtc ttgcgggtaa gctggttgcc
    23461 gcgtatggcg aaccgttgag cgagcccgat ggcgcgctga ctcacgcctt ccctcagccg
    23521 tcggcgcttg tgtcggtgga cctcgccgcg ctgggcatgc cgcgcagccg ggccgcctat
    23581 ttgtcagccg tcgccgcagc ggtggtcacc gatccagggt tgttcgatgc gacgcaaagt
    23641 ctggatgacg cggtacaaaa gctgaagggc attcgtgggg tgggcgagtg gacggcccag
    23701 tacatcgcgc tgcgtcagtt gcgtgagcct gacgcttttc cggcagccga tgttgggttg
    23761 atgcgtgcca tgggtactct cgatggggag gtgtggaccg ccgccaagct gcttgaacat
    23821 gccgaggc
//
LOCUS       DASTNK010000092        19299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_989861, whole genome shotgun sequence.
ACCESSION   DASTNK010000092 DASTNK010000000
VERSION     DASTNK010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 19299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19299
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989861"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2273
                     /gene="aceE"
                     /locus_tag="VFR20_07145"
     CDS             <1..2273
                     /gene="aceE"
                     /locus_tag="VFR20_07145"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEU0230330.1"
                     /translation="DWDPLLAKDGDGVLVNQMNTTPDGQYQTYTVETGAYIREHFFGG
                     DPRLMPEFWEFPTVSMGLGPISAIYQARFNRYLHARGLADTSNSHVWAFLGDGEMDEI
                     ESLGAIGVAAREELDNLTFVINCNLQRLDGPVRGNGKIIQELEGDFRGSGWNVIKLIW
                     GGYWDPLLARDKEGILRRVMEESVDGEYQAYKARDGKFVRDHFFGKHPKLLEMVSRMS
                     DEDVWRLNRGGHDPYKVYSAFAAASEHKDQPTVILAKTIKGYGMGQVGQAKNPTHQQK
                     KLDMESVREFRDRFNIPVPDDKLEELPYFKPADDSPEMKYLHERRGALGGYVPHRRVK
                     ADEQLKVPDLSAFKAVLEPTIEGREISTTQAFVRVLNQLLRDKTLGPRVTPILVDESR
                     TFGMEGLFRQIGIYAPEGQKYTPVDKDQVMYYREATDGQLLQEGINEAGGFSSWIAAA
                     TSYSTNNRIMIPFFAYYSMFGFQRVGDLAWAAGDMLARGFLLGGTAGRTTLNGEGLQH
                     EDGHSHILSSMIPNCVSYDPAFAHELAVIMQNGLKRMVEDQEDVFYYLTIMNENYPQP
                     GLKTGDEEGILRGMYKFKSVGKTKLRVQLMGSGTILREVLDAQALLHDDWKISSDVWS
                     VTSYTELRRDGLDCERHNLLHPEGDTKVPYVTSQLDGTDGPIISSTDYVKAFSDQIRP
                     YVPKGRTFKVLGTDGFGRSDFRSRLREHFEVNRHFVVLAALSALADEGAIPRSKVAEA
                     MKKYAIDPNKANPHHA"
     gene            2288..4012
                     /gene="aceF"
                     /locus_tag="VFR20_07150"
     CDS             2288..4012
                     /gene="aceF"
                     /locus_tag="VFR20_07150"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEU0230331.1"
                     /translation="MSNSIEIKVPDIGDFGEVEVIEVLVAEGDTVAQEQSLITVESDK
                     ASMEIPSSAAGVVRSLKTKVGDKVKEGSIILEIEPAQGGAAESSSKLAAAAPAAATPV
                     AATASVPASAAPKASAQAAPAASASGPVTVMVPDIGDAKDVEVIEIMVAVGDTIAVDQ
                     SLITLESDKASMEVPSSHAGVVTALKIKLGDKVSQGAPILEITASDSEAAAVAAPAIA
                     APAAVAPAASAPVTPAAPAPAASRASTPSLASVPPSRTSPTAAYADTAVPARNLPHAS
                     PSVRKFARELGVDLSLVTGSGEKHRITQDDIRAFVKQSLAAGGRPAAVGAATGGGLSV
                     LDWPKVDFAKFGPIESRPLSRIKKISGANLHRNWVMIPHVTNNDVADITELEALRVTL
                     NKEHQKTGPKITMLAFLIKAVVAALKKFPDFNASLDGDNLVLKQYYHIGFAADTPNGL
                     VVPVLRDADKKGVLDIAAETSELAGLARDGKLSPAQMQGGCFSISSLGGIGGTSFTPI
                     INAPEVAILGVSRSSHAPVWDGQSFQPRLMLPLSLSYDHRVIDGASAARFNAYLAALL
                     ADFRRIAL"
     gene            4023..5834
                     /gene="lpdA"
                     /locus_tag="VFR20_07155"
     CDS             4023..5834
                     /gene="lpdA"
                     /locus_tag="VFR20_07155"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU0230332.1"
                     /translation="MSTIDLAIPDIGDFGDVEVIEILVAEGDTIAAEQSLITVESDKA
                     SMEIPAERGGVVKAVLVKLGDKVSQGTPVVRIEAQAGAEAGPKGTTTEASKGDARPAE
                     VEAPAAPSAQPAAVAASAVVAPQPASSFAGTADIECEVLVLGAGPGGYSAAFRAADLG
                     LDVVLVERYSTLGGVCLNVGCIPSKALLHTAAATEEALSLASHGISFTAPAIDLDKLR
                     GYKDSVVGKLTGGLAAMARARKVRVLTGVGEFLDPFHLSVRAADGASKVVKFRSAIIA
                     AGSQSVKLPFMPNDDRVVDSTGALRLTSVPGKMLIVGGGIIGLEMGTVYSALGSRLDV
                     VEMLDGLMPGADRDLVKVWQKMNARRFDHLMLGTRTVGAEAREDGIWVTFDGPGAPEA
                     QRYDLVLQAVGRSPNGKNIGAEKAGVRVTDRGFIEVDRQMRTNVPHIHAIGDIVGQPM
                     LAHKAVHEAHVAAEVLAGQKSYFDARVIPSVAYTDPEVAWVGLTEDAARKDGIEVKKG
                     LFPWAASGRAIANGRDEGFTKLLFDASTHRIVGGGIVGTHAGDLIGEVALAIEMGADA
                     VDIGKTIHPHPTLGESMGMAAEAAEGHCTDLPPVRGK"
     gene            5910..7304
                     /gene="folC"
                     /locus_tag="VFR20_07160"
     CDS             5910..7304
                     /gene="folC"
                     /locus_tag="VFR20_07160"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU0230333.1"
                     /translation="MSLPKPGGEATLDQWLAYLESLHHVSIELGLDRVRRVAERLGLD
                     LSASVRIIVGGTNGKGSTCAMLEAILLAAGYKVGLYTSPHLIDFNERIRVNGELATDA
                     QIVEQFHEIEAGRGEISLSYFEYTTLAALRLFQKLALDVVVLEVGLGGRLDAVNIVDA
                     DCSVVTSVDMDHMEWLGDTRDKIGWEKAHIFRPERPAICADPVPPDTLVRYALDIGAD
                     LWRFGRDYNYSGDRLQWAYGGRARRRTGLGYPALRGANQLLNASAALAALEALEDRLL
                     VPQQAVRLGLAQAALPGRFQTLPGLPVVVLDVAHNPHAAAALGQNLDAMPHFPVTHAV
                     VGMLKDKDIAGVVSRLATRVDHWYCATLGGPRGTTGETLAQIVRRVTSSGDAAQSSPD
                     DSPTSGAGEGGHVVPRAVRQPRKEPISISSFPGPEQAFAAARGQAADNDRILVFGSFA
                     TVGPVLQSLSRKAD"
     gene            7356..8312
                     /locus_tag="VFR20_07165"
     CDS             7356..8312
                     /locus_tag="VFR20_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEU0230334.1"
                     /translation="MGLFSRNEPAARSNTRKNSRSSASSEAQASELRGRARRRLIGAL
                     VLIVAAVIILPVLFNKPPESGKTPVVAQVTIPATPPANSTPPASTKPTGASPGTASGG
                     AITSAPLPAPQPVQPGTGNQVASAQQPIDTAPTSEPAATSNKPAQPEKSAVSEKSPEK
                     TSTQPSEKKSGKPRESASSSKRHDNGGRDAGNHDTEKLARAVDRGPGSTVDKVKRTDN
                     GAVALALLQGRTPGQAAPATSDRGRYVLQIAAYTTKSDADSRRARLASAGVTNAYVES
                     AGSGDKHTYRLRVGPFPTREAAQAAQARLRALGYDNGFISTK"
     gene            8309..8797
                     /locus_tag="VFR20_07170"
     CDS             8309..8797
                     /locus_tag="VFR20_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU0230335.1"
                     /translation="MTSFDYIVLAILGVSAFLGLLRGLVKEVLSLIAYAAAFVAAIWW
                     GPKVSSWLVSLIDNGLLRTAAAYAAVFIVALLLVGLLNVTLGTLIEKTGLTPADHGLG
                     ALFGFLRGMLIVLALVALAGYTQLPSEPWWRDAQLSHAAVQGVRQIKLMLPPSLATWL
                     PY"
     gene            8827..10314
                     /gene="purF"
                     /locus_tag="VFR20_07175"
     CDS             8827..10314
                     /gene="purF"
                     /locus_tag="VFR20_07175"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU0230336.1"
                     /translation="MCGIVGVVGNSPVNQLIYDSLLLLQHRGQDAAGIATSHDDFFNM
                     HKAHGLVRDVFRTRNMRSLPGNSGLGQVRYPTAGSSVNVAEAQPFYVNAPFGITFVHN
                     GNLTNWRELREELFKIDRRHINTNSDSEVLLNVFAHELQLASTGVSLDEKAVYAAVAA
                     VHRRVKGGYAVIAQIAGFGVVAFRDPYGIRPLCMGSNKTDAGSEWMLASESVALTGCG
                     FTFERDVLPGEAVLIDLDGRVSGQQCAPAPILTPCVFEYVYFARPDSLMNGVSVYDAR
                     LNMGEYLADNVAKSLRLGDVDVVMPIPDSSRPSAMQLASRLNLNYREGFIKNRYVGRT
                     FIMPGQAVRKKSVRQKLSAIDMEFRGKNVLLVDDSIVRGTTSREIVDMARAAGANKVF
                     FASAAPPVRYPNVYGIDMPTRAELIAADRDVAEVAREIGADGLVYQDLADLEQSIRDL
                     NPDITQFESSCFDADYVTGDIDSAYLERLGRTRGEAAADNPEDDA"
     gene            complement(10384..10863)
                     /locus_tag="VFR20_07180"
     CDS             complement(10384..10863)
                     /locus_tag="VFR20_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEU0230337.1"
                     /translation="MTQTTGRLLHAVALLSILAVALALVSQHVFNLRPCAWCVMQRLI
                     YLCVAVVCWLGILLSRLPSLRRLAALVALLLCVGGVLAAWYQYDVAAHLFSCSQTFAD
                     RLMVGLGLDAHLPWLFGIFASCMDAQVRILGVEYALWSLGLFCVLGLMSLAALLRRA"
     gene            complement(10955..11665)
                     /locus_tag="VFR20_07185"
     CDS             complement(10955..11665)
                     /locus_tag="VFR20_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEU0230338.1"
                     /translation="MMINDFFIDFSRSFLFALATLIPILNPPAAAPIFLMLTEGIPNS
                     VRGQIAKRVAVNILIMLAVAMIAGNLVLEFFGISLPIVRVGGGLLVIASAWRLVNASD
                     LNAEHAQSMVESFSTDQIRAKAFYPLTFPMICGPGTLAAALTVGATLHDRTMAITLTK
                     LAGTLPAIIVAAVAVYFCLKFAAQFMHRLGPSGTAVFMRLSAFILLCMGVQIVWDGAQ
                     DLLLHLLIQASALHTPAG"
     gene            complement(11662..14310)
                     /locus_tag="VFR20_07190"
     CDS             complement(11662..14310)
                     /locus_tag="VFR20_07190"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEU0230339.1"
                     /translation="MPHPPIESANTLDGSFVEALRDKLARLRDEAIARFREHLRPEAL
                     LVALRRCTDQTLRELIRRHPLPKGATLAAVGGYGHGELFPHSDIDVLILLERPPTESD
                     RALLEDLVAALWDLGLDLGHSVRTIDDCQAEAGNDITVETALMEARWLCGSRELLREL
                     IRTMRAQTNPQAFFRAKRAELLQRHARYHDTPYALEPNCKESPGGLRDLQVILWLAQA
                     AGLGRTWREISRAQVLTPAEYRALRQADLAFKRLRIELHLLTGRREDRVLFDLQPALA
                     ARYGFEGTRNRRASEILMQRYYWAARVVIQLNTILIQNIEERLFPEPQTRIQPVDDDF
                     QIVHNRLDIIRDDGFDRNPTLLLRIFLVMQQHPSLQGMSARTMRALWHARRRIDSQFR
                     RNPVNRYLFLQILQQPSGIVHALRGMTMLNILPRYLPVFRRIVGQMQHDLFHVYTVDQ
                     HTLMVIRNLRRFTMPEHAQEFPLASQLMSSLDKHWLLYVAALFHDIAKGRGGDHSELG
                     AHDARRFCQDHGLAREDTDLVEFLVAQHLTMSRVAQKRDLADPAVVHDFAHKVGNERR
                     LTALYLLTVADIRGTSPRVWNSWKGKLLEDLYHLTLDALGGAQPDTHTLLAQRREAAA
                     AEIRLMGLRDDSRDAFWRELDVAYFLRHEASEIAWHTRHLYYQIDAPAPVVRVRVLSA
                     GEGLQIMVYTHDRDDLFMTICRYFDRRALSIQDARIHTTMHHWALDSFIVLAPAHESD
                     YRSLATLVEHELTNALATDEGSRGLADAPAGRLHPVSRRSRVFPITPNVELRPDDNGM
                     TWRLSITAADRPGLLHSLSRVFAHHRVRLKMAKVMTLGDRVEDIFIIEGEALEIPRTQ
                     LRFERDVLAGLDDTPSDPLRLQQEAP"
     gene            complement(14409..15224)
                     /gene="map"
                     /locus_tag="VFR20_07195"
     CDS             complement(14409..15224)
                     /gene="map"
                     /locus_tag="VFR20_07195"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEU0230340.1"
                     /translation="MLSLVTDPEDLNKMRLACQEAASVLDFITPHVQPGVTTAELDRL
                     CLDYINNTLKVKSATVGYAPPGYPPFPSSICTSVNHVICHGIPGDKVLKNGDIMNIDV
                     TIIKDGWFGDTSRMYYVGEPSILARRLAETTYECMWLGIDKVRPGATLGDIGHVIQKH
                     AEKAGFSVVREYCGHGVGRKFHQDPQVLHYGKPGTGVRLEPGLIFTIEPMINAGRKDI
                     RQLADGWTVVTRDHSLSAQWEHSILVTEDGYEVLTVSDGMPPPPAVLNASAVA"
     gene            15378..16133
                     /gene="rpsB"
                     /locus_tag="VFR20_07200"
     CDS             15378..16133
                     /gene="rpsB"
                     /locus_tag="VFR20_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU0230341.1"
                     /translation="MSLMREMLEAGVHFGHQTRYWNPKMAPFIFGQRNKIHIINLEQT
                     VALYQDATKFVRQVAAKGGTVLFVGTKRAARELVAAEAARCGMPYVDSRWLGGMMTNF
                     KTVKSSVKRLKEMEVQQAEGATERMSKKEALMFERELAKLNKSIGGIKDMNTLPDALF
                     VIDVGYHKIAVAEANTLGIPVVAVVDSNHSPDGIAYVIPGNDDSAKAIALYAHGMADA
                     VLEGREQNLNGLVEEIGEGGEEFIEVESEPEQE"
     gene            16266..17144
                     /gene="tsf"
                     /locus_tag="VFR20_07205"
     CDS             16266..17144
                     /gene="tsf"
                     /locus_tag="VFR20_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEU0230342.1"
                     /translation="MAQITAALVKELRERTDAPMMECKKALTEADGDLARAEELLRVK
                     LGNKASKAAGRITAEGLVGLFISPDAKQGAVVEINCETDFVARNDDFIGFVNQVAELV
                     SAKNPADLTALSALPMAGATVEATRTALVGKIGENISVRRFQRFQTNDQLASYVHGGR
                     IGVLVEFSGSAEIGKDLAMHIAATKPRALDANGVSAEDIAAERSVAEQKAAESGKPAE
                     IITKMVEGSIQKFLKEVTLMSQPFVKNDKQSVAQMLKEKGASISQFALYVVGEGIEKK
                     SEDFAAEVAAAAGGAA"
     gene            17235..17951
                     /gene="pyrH"
                     /locus_tag="VFR20_07210"
     CDS             17235..17951
                     /gene="pyrH"
                     /locus_tag="VFR20_07210"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU0230343.1"
                     /translation="MTTSSYKRVLLKLSGEALMGDDAFGINRATILRMTEEIAEVVVR
                     GVELAIVIGGGNIFRGVAPGAQGMDRATADYMGMMATIMNALALQDALKNRGVDARVQ
                     SALNIDQVVEPYIRPKALRYLEEGKVVVFAAGTGNPFFTTDTAAALRGAEIGAEIVLK
                     ATKVDGIYSADPNKDASATRYSRISFDEAIVRRLEVMDATAFALCRDQKLPVKVFSIN
                     KPGALKRVVAGEDEGTLVHV"
     gene            17966..18526
                     /gene="frr"
                     /locus_tag="VFR20_07215"
     CDS             17966..18526
                     /gene="frr"
                     /locus_tag="VFR20_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU0230344.1"
                     /translation="MTPSEIKKSAEDRMNKSLEALQTGLSKLRTGRAHTGILDHVQVE
                     YYGSPVPVSQVSNVTLLDARTISVQVWEKNMAGPVEKAIRESDLGLNPIPMGDNIRVP
                     MPALTEERRRDLTKVARGEGEEAKIAIRNLRREANDTLKKLVKEKEISEDQERRSQEE
                     IQKLTDKFVLDVDKLVAQKDAEIMTV"
     gene            18569..>19299
                     /gene="uppS"
                     /locus_tag="VFR20_07220"
     CDS             18569..>19299
                     /gene="uppS"
                     /locus_tag="VFR20_07220"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEU0230345.1"
                     /translation="MQASSTQTIPERSDVPRHLAIIMDGNGRWATQRFLPRTAGHARG
                     VQAVRRIVEACGRRGVRYLTLFAFSSENWRRPQEEVSLLMRLFIQTLEKEVGKLEEQG
                     VRLRVVGDTSAFEPRLQSLIRLVEERTAHNALLNLTVAANYGGRWDILQAMRRMLAAE
                     PGLSNQPDDIDEARLAPHLSMAWAPEPDLFIRTGGEQRISNFLVWQLAYTELYFTDSY
                     WPDFGAKELDAAFEWYRTRERRFGRT"
BASE COUNT         3697 a         5922 c         6190 g         3490 t
ORIGIN      
        1 gggactggga tccgctgctg gccaaggacg gcgacggcgt gctggtgaac cagatgaaca
       61 ccacgccgga cgggcagtac cagacataca ccgtcgagac cggcgcctac atccgcgagc
      121 acttcttcgg cggcgacccg cggctgatgc cggagttctg ggagttcccg accgtctcga
      181 tgggcctcgg cccgatcagt gcgatctacc aggcccgctt caaccggtac ctgcacgcgc
      241 gcgggctcgc cgacacgtcc aactcgcacg tatgggcctt cctcggcgac ggcgagatgg
      301 acgagatcga gtcgctcggc gcgatcggcg tggcggcccg cgaagagctg gacaacctga
      361 ccttcgtcat caactgcaat ctgcagcgcc tcgacggccc ggtccgtggc aatggcaaga
      421 tcatccagga actcgagggc gacttccgcg gcagcggctg gaacgtcatc aaactcatct
      481 ggggcggata ctgggatccc ctgctggcgc gcgacaagga aggcatcctt cgccgtgtca
      541 tggaagagtc ggtcgatggc gaataccagg cctacaaggc gcgtgacggc aagttcgtgc
      601 gcgaccactt ctttggcaaa catccgaagc tgctcgagat ggtcagccgc atgagcgacg
      661 aagacgtctg gcgcctgaat cgtggtggcc acgaccccta caaggtgtac tcggccttcg
      721 ccgccgccag cgagcacaag gaccaaccca ccgtcattct cgccaagacc atcaaaggct
      781 atggcatggg tcaggtgggg caggccaaga acccgacgca ccagcagaag aagctcgaca
      841 tggaatcggt gcgcgaattc cgcgaccgct tcaacattcc cgttcccgac gacaaactcg
      901 aagagctgcc gtatttcaag ccggcggatg attcccccga aatgaaatac ctgcacgagc
      961 gtcgtggcgc cctgggcggt tatgtgccgc accggcgcgt caaggcggac gagcagctca
     1021 aggtgcccga tctctccgcg ttcaaggcgg tgctggagcc caccatcgag ggccgggaga
     1081 tctccacaac ccaggctttc gtccgggtcc tgaaccagct cctgcgtgac aaaaccctcg
     1141 ggccgcgcgt caccccgatc ctagtcgacg aatcacgcac tttcggcatg gagggtctgt
     1201 tccgccagat cggcatctac gcgccggaag gccagaagta cacgccggtc gacaaggacc
     1261 aggtcatgta ctaccgcgag gcgaccgacg ggcagcttct ccaggaaggc atcaacgagg
     1321 ccggtggctt cagctcctgg atcgcggcgg ccacgtcgta ctccacgaac aaccgcatca
     1381 tgatcccgtt cttcgcgtat tactcgatgt ttgggttcca gcgcgtcggc gacctggcct
     1441 gggcggcggg cgacatgctg gcccgcggct tcctgctggg cggcaccgcc ggccgcacca
     1501 cgctcaatgg cgagggcctg cagcacgaag acggccatag ccacatcctg tcgtccatga
     1561 tccccaactg cgtgtcgtac gatcccgcct tcgcgcacga gctggcggtc atcatgcaga
     1621 acggtctcaa gcgcatggtc gaggatcagg aggatgtctt ctactacctg acaatcatga
     1681 acgagaacta ccctcagcca gggctcaaaa cgggcgacga agagggtatt ctgcgcggca
     1741 tgtacaagtt caagtcggtg ggcaagacca agctgcgcgt ccagctcatg gggtccggca
     1801 ccatcctgcg tgaagtgctc gatgcccagg cgttgctgca cgacgactgg aagatcagct
     1861 ccgatgtctg gagcgtcacg agctacaccg aactgcgtcg ggatggcctg gattgcgaac
     1921 gccataatct gttgcacccc gaaggcgaca ccaaagtgcc ttacgtcacc agccagctcg
     1981 atggcaccga cggccccatc atctcgtcca ccgattacgt caaggcgttc agtgaccaga
     2041 tccggccata cgttcccaag gggcgcacgt tcaaggttct gggcaccgat ggtttcggcc
     2101 ggtccgattt ccgatccagg ctgcgtgaac acttcgaggt gaatcgccac ttcgtggtgc
     2161 ttgccgcgct cagcgcgctg gccgacgagg gcgccatccc gcgctccaag gtcgccgagg
     2221 ccatgaagaa atacgcgatt gatcccaaca aagccaaccc gcatcacgca tgacggggga
     2281 gacaagcatg agcaacagca ttgaaatcaa ggtgccggac atcggcgatt tcggcgaggt
     2341 cgaagtcatc gaagtattgg tggctgaagg ggacaccgtt gcccaggaac aaagcctgat
     2401 cacggtggaa tccgacaagg cgtccatgga gatcccgtct tccgcggccg gtgtggtccg
     2461 ttcactcaag acgaaggtcg gtgacaaggt caaagagggt tcgatcattc tcgagatcga
     2521 gccggcccag ggtggggcgg cggaatcttc atcgaaactg gcagccgctg cccccgctgc
     2581 ggcgaccccc gttgccgcga cggcctccgt gcccgcttcc gcagcgccca aggcatccgc
     2641 ccaggccgcg cctgcggcaa gcgcctcggg gcctgtcacc gtgatggtgc ccgacatcgg
     2701 cgatgccaag gatgtcgaag tcattgagat catggtcgcg gttggcgaca ccatcgcggt
     2761 ggatcagagc ctgatcacgc tggagtccga caaggcgtcc atggaggttc cctcgtctca
     2821 tgccggtgtg gtcaccgcgt tgaagatcaa gctcggtgac aaggtcagcc agggcgcgcc
     2881 cattctggaa atcactgcga gcgactcgga agccgccgcc gtggccgctc ccgcgattgc
     2941 cgcgcccgcc gccgtggcac ccgccgcatc ggcaccggtg accccggccg cacccgcgcc
     3001 agctgcatca cgcgcttcga cgccatcgct ggcctcggtg ccgccctcgc gcacctcccc
     3061 gacggcggct tacgccgaca ccgccgttcc ggcgcgcaat ttgccgcatg cgtccccgtc
     3121 ggtgcgcaaa ttcgcccggg agctgggtgt cgacctctcc ctggtgaccg gatccggtga
     3181 aaagcaccgt attacccaag acgacatccg cgcgttcgtc aaacaatccc tggctgccgg
     3241 tggccgtccc gcggcggttg gcgcggcaac gggaggcggg ctgagcgtcc tggactggcc
     3301 caaggttgac ttcgccaaat tcggcccaat cgaatcgcgg ccgttgtcac gcatcaagaa
     3361 gatctcaggg gcgaacctgc atcgcaactg ggtcatgatt ccgcatgtca ccaacaatga
     3421 cgtggccgac atcaccgagc tcgaggccct gcgcgtcacg ttgaacaaag agcatcagaa
     3481 aaccggcccc aagatcacga tgctggcgtt cctgatcaag gcggtggtcg ccgccttgaa
     3541 gaaatttccc gatttcaacg cttcgctcga tggggacaat ctggttctga agcagtatta
     3601 ccacatcggc ttcgcggctg acacgcccaa cgggctggtc gtacccgtgc tgcgtgacgc
     3661 cgacaagaaa ggggtgctgg atatcgcggc cgaaacctcg gaactcgcgg gcctggcgcg
     3721 tgacggcaag ctgtcgccgg cgcagatgca gggcggttgc ttctccattt cttcccttgg
     3781 ggggattggc gggacgtcgt tcacacccat catcaacgca cccgaggtcg ccatattggg
     3841 tgtttcgcgt tcgtcacacg ctcccgtgtg ggatggtcaa tccttccagc ctcgcctgat
     3901 gttgcctttg tcgctgtcct acgaccaccg ggtcatcgat ggggcgtcgg ctgcgcgttt
     3961 caacgcgtat ctcgcggcgc tgctggccga tttccgccgc atcgcgctat aggagcccat
     4021 cgatgagcac gattgatctg gccatacccg acattggtga tttcggcgat gtggaagtca
     4081 tagaaatcct ggtggccgaa ggcgacacga tcgcggccga gcagagcctc atcaccgtgg
     4141 aatccgacaa ggcgtccatg gaaattcccg ccgagcgcgg cggcgtggtg aaggcggtac
     4201 tcgtgaagct gggtgacaag gtgtcgcaag gcacgcccgt cgtgcgcatc gaagcgcaag
     4261 cgggcgccga ggcggggccg aaaggcacca ccacggaggc atccaaaggc gacgcccggc
     4321 ctgcggaggt cgaggcacct gccgcgccct ccgcgcagcc ggccgcggtc gctgcttcag
     4381 cggtcgtggc gccgcaaccg gcttcgtcgt tcgccggcac ggccgacatc gaatgtgaag
     4441 ttctggttct cggggctggt ccgggagggt attccgccgc gttccgcgcc gccgacctcg
     4501 gcctggacgt ggtgctggtc gagcgttaca gcaccctggg tggtgtgtgc ctgaatgtgg
     4561 ggtgcattcc gtccaaggct ttgctgcaca ccgccgcggc caccgaggaa gcactgtcgc
     4621 tggcgtccca cggtatttcg ttcacggcgc cggccatcga tctggacaag ttgcgcggct
     4681 acaaggacag cgtggttggc aaactcaccg gcgggttggc ggcgatggcc cgtgcccgca
     4741 aggtgagggt gctgaccggg gtgggggagt ttctcgaccc gttccatttg tccgtacggg
     4801 ccgccgacgg agcaagcaag gtggtgaagt tccgttcggc catcatcgcc gcgggtagcc
     4861 agtcggtcaa attgcccttc atgccgaacg acgaccgcgt cgtggattcg acgggcgcgt
     4921 tgcgcctgac gtccgttccc gggaagatgc tcatcgtcgg aggcggcatc atcggccttg
     4981 aaatgggaac ggtgtattcg gcgctgggca gtcgcctgga cgtggtcgaa atgctggatg
     5041 gcctcatgcc gggggccgac cgcgacctcg tcaaggtgtg gcagaaaatg aatgccaggc
     5101 gcttcgatca tctcatgctg ggcacccgga ctgtcggcgc cgaggcgcgt gaagacggga
     5161 tatgggtgac attcgacggg ccgggggccc ccgaggccca gcgttacgat ctggttctgc
     5221 aagccgtcgg ccgctcgccg aacggcaaga atatcggtgc cgagaaggcg ggcgtgcgcg
     5281 tcaccgatcg cggattcatt gaagtcgatc ggcagatgcg caccaatgtg ccgcacatcc
     5341 acgccatcgg tgacatcgtc ggccaaccca tgctggcgca caaggcggtc cacgaggcgc
     5401 acgtggcggc cgaagtcctt gctgggcaga agagctactt cgatgcccgg gtcattccgt
     5461 ccgtggcgta caccgacccc gaggtggcct gggtcggcct gaccgaggat gccgcgcgca
     5521 aggacggcat cgaggtcaaa aaggggttgt tcccctgggc ggcgtcgggc cgcgccatcg
     5581 ccaatggccg ggacgagggt ttcaccaagc tgttgttcga tgcgtccacg catcgcatcg
     5641 tgggaggcgg cattgtcggc acgcacgcgg gcgatctcat cggcgaggtc gctctggcca
     5701 tcgagatggg cgccgatgcg gtcgatatcg gcaagaccat ccatccccat cccacgttgg
     5761 gcgaatccat gggaatggcg gccgaggcgg cggagggcca ttgcaccgat cttccgccag
     5821 tgcgcggcaa gtaagggtat caccgttgct tctcgtggcg ggtgctatca tcggcacccg
     5881 ccttttcttt tttctcagcg gtcatcatca tgtctcttcc caaaccaggt ggcgaggcca
     5941 cactggacca gtggctggcc tatctcgagt cgttgcatca tgtatcgata gaactgggcc
     6001 tggatcgggt gcgccgggtt gccgaacgcc tgggtctcga tctgtcggcg tcggtcagga
     6061 tcatcgtcgg cggcaccaac gggaaggggt ccacctgcgc catgctggag gcgattctgc
     6121 tggcggccgg atacaaggtc ggcctgtaca cctccccgca tctcatcgat ttcaacgagc
     6181 gcatccgcgt caatggcgaa ctggcgaccg acgcacagat cgtcgagcag tttcacgaga
     6241 tcgaggcagg gcggggggaa atatcgctca gttatttcga atacacgacg ctggccgcgc
     6301 tgcgcctgtt ccagaagctg gcgctggacg ttgtggtgct tgaggtcggc ctgggaggcc
     6361 ggctcgatgc cgtgaacatt gtcgatgccg actgttcggt ggtgaccagt gtcgacatgg
     6421 accacatgga atggcttggt gacacgcgtg acaaaatcgg ctgggagaaa gcgcacattt
     6481 tccgcccgga gcgcccggcg atctgtgccg atcccgtgcc cccggacacg cttgtccggt
     6541 acgcgctgga catcggtgcg gatctgtggc gtttcggtcg tgattacaac tattcgggcg
     6601 accgtctgca atgggcctac ggaggtcgtg ccagacgtcg cacggggctg ggctatccgg
     6661 cgttgcgcgg cgccaatcag ttgctgaacg cctcggccgc gttggcggcg cttgaggccc
     6721 tggaagaccg gttgctggtg ccgcaacagg ccgtgcgcct gggcctggcc caggccgcgt
     6781 tgcctggccg cttccagact ctgcctgggc tgcctgtcgt ggttctcgat gtggcacata
     6841 acccgcatgc cgcagccgcg ctggggcaga atctggatgc catgccccat ttcccggtga
     6901 cgcatgctgt ggtgggcatg ctcaaggaca aggacatcgc cggtgtcgtg tcacgtctgg
     6961 ccacacgtgt ggaccactgg tattgcgcga cgctgggcgg tccgcgtggc acgacaggag
     7021 aaaccctggc acaaatcgtt cggcgggtga cgtcgtccgg ggatgccgct cagtcgagtc
     7081 ctgacgattc gccaacttcg ggggcgggcg agggcgggca cgtggtgcct cgagcggtgc
     7141 ggcagccacg caaagagccg atatcgattt caagcttccc aggtcccgag caggcgttcg
     7201 ccgctgcgcg cggacaggcg gcggacaatg atagaattct cgtgtttggc tcattcgcca
     7261 ctgtgggtcc ggtgctgcaa tcgctttctc gcaaggctga ttgacattcc gggcagtgcc
     7321 ggcccgggcc cgttcgccag tcagggtttt gctttatggg attgttctct cgcaacgaac
     7381 cggccgccag gtccaacaca cgtaagaact cgcgcagttc tgcgtccagc gaggcgcaag
     7441 cgtccgagct cagggggcgt gcaaggcgtc gcctgatcgg cgcgctggtg ttgatcgtcg
     7501 cagccgtgat cattcttccg gtgttgttca acaagccgcc agaaagcggc aagacgccgg
     7561 tggtggcgca agtgaccatt cccgccacac cgccggccaa ttccacgcca cccgcctcca
     7621 cgaaaccgac tggcgcctcg cccggcaccg cgagcggggg agcgatcact tcggcgccac
     7681 tgcctgcgcc gcaacctgtg cagcccggca ccggcaacca ggtcgcaagc gcccaacagc
     7741 ccatcgacac cgcgccaacc tccgaaccgg ccgccacgtc caacaagccg gctcagccgg
     7801 aaaagtcggc ggtatccgaa aagtccccgg aaaagacatc cacgcagccc tcagagaaaa
     7861 aatccgggaa accgcgtgaa tccgcctctt catccaagcg gcatgacaac ggcggccgcg
     7921 acgccggcaa ccacgacacg gaaaaactcg ccagggctgt tgatcgcgga cccgggtcca
     7981 cggtggacaa ggtcaaacgc actgataatg gcgctgtcgc gctggcattg ttgcagggcc
     8041 gcacccctgg ccaggccgcg ccggccacga gcgatcgtgg cagatatgtg ttgcagatcg
     8101 ctgcctatac caccaagagc gatgctgatt cacggcgcgc ccgtcttgct tcggcgggtg
     8161 tcaccaatgc ttatgtcgag tcggctggat cgggcgacaa gcacacatac cgcctgcgtg
     8221 tcgggccgtt tcccacccgc gaggccgctc aggcggctca agccaggttg cgcgcattgg
     8281 gatatgataa cggcttcatc tcgaccaagt gaccagtttt gactacatcg tcctggccat
     8341 tctcggtgta tcggcctttc tggggctgct gcgcgggttg gtcaaagaag ttctctccct
     8401 gatcgcttac gccgcggcat tcgtggccgc catctggtgg gggccgaaag tgtcctcatg
     8461 gctggtttcc ctcatcgaca atggcttgct tcgcaccgcc gccgcttatg cggcggtatt
     8521 cattgtggcc ctgctgttgg tggggctgct caacgtgacg cttggcactc tcatcgagaa
     8581 aaccgggctg acgccggccg accatggtct gggtgccttg tttgggtttc tgcgcggcat
     8641 gctgatcgtg ctggcgctgg tggcgcttgc gggctatacc cagttgccca gcgagccgtg
     8701 gtggcgcgat gcgcagttgt cccatgccgc ggtgcagggg gtgcggcaga tcaaacttat
     8761 gctgccgcca tcgctggcca cctggctgcc gtattgaacg ggtttcaaag ctaacaggga
     8821 ttcatcatgt gtggaatcgt cggggtcgtg ggaaacagcc ctgtgaatca attgatttac
     8881 gacagtctgc tgttgctgca gcatcgcggg caggatgcgg cgggtatcgc cacttctcac
     8941 gatgatttct tcaacatgca caaagcgcat ggactggtgc gcgacgtgtt tcgcacccgg
     9001 aacatgcgct cgttgccggg caacagcggg ctggggcagg tgcgctaccc caccgccgga
     9061 tccagtgtca acgtggcgga agcccagccg ttctatgtca acgctccctt tggcatcacg
     9121 ttcgtgcaca acggcaatct gacaaactgg cgcgagttgc gcgaagagtt gttcaagatc
     9181 gaccgtcgtc acatcaacac gaactccgac tccgaggtgt tgctcaacgt attcgcccat
     9241 gaattgcagt tggcctcgac gggggtgtct ctggacgaga aggcggtgta tgccgctgtc
     9301 gccgccgtcc atcggcgcgt caagggtggg tatgcggtga tcgcccagat cgcgggattc
     9361 ggcgtcgtcg cgttccgcga tccctatggc atccgcccat tgtgcatggg gtcgaacaag
     9421 accgacgccg gttcggaatg gatgctggcg tccgagtccg tggccttgac ggggtgcggg
     9481 ttcacctttg aacgcgacgt gctgcctggc gaggcggtgc tcatcgacct ggacggccgt
     9541 gtcagtggcc agcaatgcgc gccggctccg atcctgacgc cttgcgtgtt cgaatatgtg
     9601 tatttcgccc ggcccgattc cctgatgaac ggggtttccg tgtacgacgc ccggctcaac
     9661 atgggtgaat acctcgccga caacgtcgcc aaatcattgc gtctgggtga cgtggacgtc
     9721 gtcatgccca tccccgattc ttcacggcca tcggcaatgc aactcgcgtc acgcctgaac
     9781 ctgaactacc gcgaagggtt catcaagaac cgttatgtgg gccgtacctt catcatgccg
     9841 ggccaggccg tcaggaagaa atccgttcgg cagaaactca gcgccatcga catggagttt
     9901 cgcggtaaga atgtgctgct cgtcgacgac tccatcgtgc gcggcaccac cagccgtgaa
     9961 atcgttgaca tggcgcgcgc tgccggggcg aacaaagtgt ttttcgcgtc ggccgcccct
    10021 cctgtgcgct atccgaatgt ctacggcatc gacatgccga cccgggcgga gctcatcgcc
    10081 gccgaccgtg atgtggcgga agtggcgcgc gaaatcggcg cggatggcct ggtctaccag
    10141 gatctggccg atctggaaca atccattcgc gatctgaacc ccgacatcac acaattcgag
    10201 tcgtcgtgtt tcgacgcgga ctatgtgacc ggggatatcg acagtgcgta tctcgaacgc
    10261 ctgggacgca cccgcgggga ggccgcggcc gacaatccgg aagacgacgc ctgagggttc
    10321 gtccggaaga caacgcctga gggttcgtca cctccctccc ggccgcacgc cgggaggccg
    10381 gtttcaggcc cgccgcagca gcgccgcgag gctcatcagc cccagcacgc agaacagccc
    10441 caggctccat aaggcgtact cgacgcccag aatcctcacc tgcgcatcca tgcaggaagc
    10501 gaaaatgccg aacaaccagg gtaagtgggc atccagtccc agccccacca tgagacgatc
    10561 ggcgaacgtc tggctgcacg agaacagatg ggcggccacg tcatactggt accaggccgc
    10621 cagcaccccc ccgacgcaca gcaacaaggc caccagggcc gccaatcgac gcaaggacgg
    10681 cagccggctc agcaatatcc ccagccagca cacaacggcc acacagaggt agatcagccg
    10741 ctgcatcacg caccaggcgc aggggcgcag attgaacacg tgctgggaca ccagcgcgag
    10801 ggcgacggcg agaatactga gcagggccac ggcgtggagc aggcggccgg tggtttgagt
    10861 catgatgcgt cccaaggaag tttgttggtg ggtaaagcgt gttcaaatcg gcgacggtgc
    10921 ggcgtggcgt gccggtacgg tttgttctct gtgatcagcc cgcaggagtg tgcagcgccg
    10981 atgcctggat gagcaggtgc agcagcagat cctgcgcgcc atcccagacg atctgcaccc
    11041 ccatgcaaag caggatgaat gcagacaggc gcatgaacac cgcggttccg ctgggtccga
    11101 gccggtgcat gaactgtgcc gcgaatttca ggcagaagta tacggccacc gcggcgacga
    11161 tgatggcggg cagggttccc gccaacttgg tgagtgtgat cgccatcgtt ctgtcgtgca
    11221 atgtcgcgcc cacggtcaat gccgcggcca gtgtgcccgg cccgcagatc atggggaacg
    11281 tgagagggta gaacgccttg gccctgatct ggtccgtgct gaaagattcg accatgcttt
    11341 gcgcgtgctc ggcattgaga tccgacgcgt tcaccaggcg ccatgcgctg gcgatgacca
    11401 gcaggcctcc accgacgcgc acgatgggca gggaaatgcc gaagaattcc agcaccaggt
    11461 tgccagcgat catggcgacc gccagcatga ttaggatatt caccgccacc cgtttggcga
    11521 tctggcccct gacgctgttc ggaatgcctt cggtcaacat caggaagatc ggcgcggcag
    11581 ccgggggatt cagtatggga atcagcgtcg cgagcgcgaa caggaagctg cggctgaaat
    11641 cgatgaagaa gtcgttgatc atcatggcgc ttcttgttga agtcggagcg gatcggaggg
    11701 tgtgtcgtca agcccggcga gcacgtcgcg ctcgaatcgc agctgggttc gaggtatttc
    11761 cagtgcctcg ccctcgatga tgaaaatatc ctcgacccgg tcgccaagcg tcatgacttt
    11821 cgccattttc aggcgcacgc gatggtgggc gaagacacgc gacaggctgt ggagcaatcc
    11881 aggcctatcc gcggcagtga tggaaagccg ccaagtcatg ccattgtcgt cagggcgcag
    11941 ttctacattg ggtgtgattg ggaagacgcg ggaacggcgt gaaacgggat gtagtcgacc
    12001 ggccggcgca tcggccaggc cgcgggaacc ctcgtcggtg gcgagggcgt tggtcagctc
    12061 gtgctcgacc agtgtcgcca gggaccggta gtcgctttca tgcgccggag ccagcacgat
    12121 gaagctgtcg agtgcccaat gatgcatcgt cgtgtggatg cgggcatcct gaatgctcaa
    12181 tgcccgccga tcgaagtatc ggcatatcgt catgaaaaga tcgtcccgat cgtgggtgta
    12241 gaccatgatc tgcagccctt cgcccgcgct gagcacacgc accctcacaa ccggggcggg
    12301 ggcgtcgatc tgatagtaca agtgccgagt gtgccaggcg atctcgctgg cttcgtggcg
    12361 caggaaatag gccacgtcca gctcccgcca gaaggcgtcg cggctgtcgt cacgcagccc
    12421 catcagccga atttcggcgg cggcggcctc gcggcgttgg gcgagcagag tgtgcgtatc
    12481 gggctgggcg ccgcccaggg catccagggt gaggtgatac aaatcttcca gcagcttgcc
    12541 tttccatgag ttccagaccc tggggctggt gccgcggatg tcggccacgg tcagcaggta
    12601 cagcgccgtc agccgacgct cgttcccaac cttgtgggcg aaatcatgca cgacggcggg
    12661 gtccgccaga tcgcgcttct gcgccacgcg ggacatggtc agatgttggg cgaccaggaa
    12721 ttcgacgaga tccgtgtctt cccgcgcaag gccatgatcc tggcagaacc gccgggcatc
    12781 gtgggcaccc agttcggaat gatctccccc ccggcccttg gcgatgtcat ggaagagggc
    12841 cgccacatac agcagccagt gcttgtcgag gctcgacatg agttggctgg ccaaggggaa
    12901 ttcctgggca tgttcgggca tggtgaaacg acgcagattg cgtatcacca tgagcgtgtg
    12961 ctggtcgacc gtgtagacgt ggaacagatc atgctgcatc tgccccacga tgcgccggaa
    13021 gacgggaagg tagcggggca ggatgttcag catcgtcatg ccgcgcagcg catgaacgat
    13081 gccgctgggc tgttgcagaa tctgcagaaa caggtaacga ttgaccgggt tgcgccggaa
    13141 ttggctgtct atgcgccgcc gcgcgtgcca cagggcgcgc atcgtgcgcg ccgacatgcc
    13201 ctgcagcgag ggatgctgtt gcatgaccag gaaaatgcgc aacaacaggg tcgggttccg
    13261 atcgaacccg tcatcgcgga tgatgtccag ccggttgtgc acgatctgga agtcgtcatc
    13321 gaccggctgt atgcgcgtct ggggctcggg gaagagccgc tcttcgatgt tctgtatcag
    13381 aatcgtgttg agctggatca ccacgcgcgc ggcccagtaa tagcgctgca tcaggatctc
    13441 gctggcccgc cgattgcggg tgccctcgaa accatagcgg gccgccaacg cgggttgtag
    13501 atcgaaaagc acgcggtcct cgcgccgtcc cgtcaggaga tgcagttcga tgcgcaggcg
    13561 tttgaacgcc aggtcggcct gccgcaacgc gcgatattcg gcaggggtca acacttgggc
    13621 gcgggagatc tcgcgccagg tgcgccccag accggcggcc tgagccagcc acaggatcac
    13681 ctgcaggtcg cgcagtccgc cgggtgattc cttgcaattg ggttcgagtg catacggggt
    13741 gtcatggtag cgcgcgtggc gttgcagcaa ttcggcgcgc ttggcccgga aaaacgcctg
    13801 gggatttgtt tgtgcgcgca tggtgcggat cagttcgcgc agcagctctc tgctgccgca
    13861 cagccagcgc gcctccatga gcgccgtttc caccgtgatg tcgttgcccg cctcggcctg
    13921 gcaatcatcg atggtgcgta cgctgtgccc caggtccagg cccagatccc acagtgccgc
    13981 caccagatcc tcgagcaggg cgcggtcgct ctcggtgggt gggcgctcga gcaggatgag
    14041 cacatcgatg tcggagtggg gaaagagctc gccgtgacca tagccgccca ctgcggccag
    14101 cgtcgcgccc ttgggtagcg ggtggcgccg gatcaactcg cgcagcgttt gatcagtgca
    14161 gcgccgcaag gcgacaagaa gggcctcggg ccgcagatgt tcgcggaacc gcgcgatggc
    14221 ctcgtcgcgc agccgggcca gtttgtcgcg cagggcttcg acaaacgagc catccaatgt
    14281 gttggcggac tcgatggggg gatgcggcat ggggtgcgat ccggttagtg ccggttcacg
    14341 ccaacgaaag agccccgacg gccctccgtg tgacggcggc tcggcgcccc gacctccggg
    14401 tcattgcttc acgcgacggc cgacgcgttc agcacggctg gcggaggcgg catgccgtcg
    14461 gacacagtga ggacctcgta gccatcttcc gtgaccagaa tgctatgctc ccattgcgct
    14521 gagaggctgt ggtcgcgtgt gacgaccgtc cagccgtcgg cgagctggcg gatatccttg
    14581 cggcccgcgt tgatcatggg ctcgatcgtg aaaatcagac cgggttcaag ccggacacca
    14641 gtgccgggtt tgccataatg cagcacctgg gggtcttggt gaaacttccg gccgacgcca
    14701 tggccgcaat attcacgcac caccgaaaag ccggcttttt cagcgtgttt ctggatgaca
    14761 tggccgatat cgcccagtgt ggcacctggc cggaccttgt ctatgcccag ccacatgcat
    14821 tcgtaggtgg tttcggcgag ccgccgcgcc agtatggagg gttcgcccac atagtacatg
    14881 cggctggtgt caccgaacca gccatccttg atgatggtga cgtcgatgtt catgatgtcg
    14941 ccgttcttca ggaccttgtc accggggatg ccgtggcata tgacatggtt gaccgacgtg
    15001 cagatggagc tcggaaacgg cgggtatcca ggcggcgcat accccaccgt ggccgatttc
    15061 accttcagtg tgttgttgat gtaatcaaga cacaagcgat cgagttcggc ggtggtgacg
    15121 ccaggttgaa cgtggggtgt gatgaaatcc aggacgctcg cggcctcttg gcaggccagc
    15181 cgcatcttgt tcaggtcttc cgggtcggta acgaggctga gcatggcaac ttgaggaaaa
    15241 tgtgctaaag atagtagaat tataggcttt cttgaagttt aagaaagaga ttcgttattc
    15301 gcgtgcgtgg ccacgacagg gtgtccggat tttccggata caggcttcgt acaagaccca
    15361 accctcggag aaatcaaatg tccctgatgc gtgaaatgct ggaagcgggt gtgcactttg
    15421 gccaccagac tcgttactgg aacccgaaaa tggcgccgtt cattttcggc cagcgtaaca
    15481 agattcatat catcaacctg gaacaaacgg ttgccctgta ccaggatgcc acgaagttcg
    15541 tgcgccaggt ggcggccaag ggcggcacgg tgctgttcgt gggcaccaag cgcgctgccc
    15601 gcgaactggt cgccgccgaa gcggcccgtt gcgggatgcc ttatgtcgat agccgctggc
    15661 tcggcggcat gatgaccaat ttcaagacgg tcaagagttc ggtgaagcgc ctgaaggaaa
    15721 tggaagtcca gcaggcggaa ggcgcgaccg agcgcatgag caagaaagaa gcgctcatgt
    15781 tcgaacgtga actggccaag ctgaacaagt cgatcggcgg catcaaggac atgaacacgc
    15841 tgcccgacgc gctgttcgtg atcgacgtgg gttatcacaa gatcgcggtg gcggaagcca
    15901 acaccctggg cattcccgtg gtggccgtgg tcgattccaa ccattcgccg gacggcatcg
    15961 cgtatgtgat ccccggtaac gacgattccg ccaaggcgat cgcgctgtac gcgcacggca
    16021 tggccgacgc cgtgcttgaa ggccgcgaac agaacctgaa tggccttgtc gaggaaatcg
    16081 gcgaaggcgg cgaggaattc atcgaggtcg aatccgagcc tgagcaagag taatcgactg
    16141 agcgcatccg ggcaggcccc ggatgcgttt cacgccccgc gcgagggtag attggcggga
    16201 tgtgcgcatc ccgctttgat ttttcgcgcg cgggcatggt gacaatctgg aactggagaa
    16261 atagagtggc tcaaattacc gctgcattgg tcaaagaatt gcgcgagcgc accgatgccc
    16321 ccatgatgga atgcaagaaa gcgctgaccg aagccgacgg cgatctagcc cgggccgagg
    16381 aattgttgcg tgtgaaactc ggcaacaagg ccagcaaggc cgccggccgc atcacggccg
    16441 aaggcttggt cggactgttc atctcgccgg atgccaagca gggcgccgtg gtcgaaatca
    16501 actgcgaaac cgactttgtg gcccgcaacg acgatttcat cggtttcgtc aatcaagtgg
    16561 ctgaactggt gtcggccaag aatcccgcag acctgacggc tctttctgcc ctgcccatgg
    16621 cgggtgccac tgtcgaagcg acccgtacgg cgctggttgg caagatcggc gaaaacattt
    16681 cggtgcgccg tttccagcgt ttccagacga acgaccaatt ggcgagctac gtgcatggtg
    16741 gccggatcgg cgtgctggtt gaattctcgg gatccgccga aatcggcaag gacctggcca
    16801 tgcacatcgc cgcgaccaaa cctcgcgcgc tcgacgccaa cggcgtgtcc gcggaagaca
    16861 tagccgccga acgctcggtg gccgagcaga aggcggccga atctggcaag ccggctgaaa
    16921 tcatcaccaa gatggtggaa ggctccattc agaaattcct caaggaagtc acgctgatgt
    16981 cgcaaccgtt cgtcaagaac gacaagcaaa gcgtggccca gatgctcaaa gaaaagggcg
    17041 cctccatcag ccagttcgcg ctgtacgtgg tgggcgaggg catcgagaag aaatccgagg
    17101 atttcgccgc ggaagtcgcg gcggcggctg gcggcgcggc ctgaggccat ttgaaacacg
    17161 gcggacgccc tccggcatcc gctgtgcgcg tatcctggca tacactttcg ttggattgtt
    17221 tctcgaggcc attcatgacc accagctcgt acaagcgtgt tcttctcaag ctatccggcg
    17281 aagcattgat gggtgacgat gccttcggca tcaatcgcgc caccatcctg cgcatgactg
    17341 aggaaatcgc cgaggtcgtg gtgcgcgggg tcgagctggc catcgtcatt ggtggcggca
    17401 acatttttcg cggtgtggcc cctggcgccc agggcatgga tcgcgccacg gcggactaca
    17461 tgggcatgat ggctacgatc atgaacgcgc tggcgctgca ggatgcgctc aagaaccggg
    17521 gggtggacgc ccgggtgcaa tccgccctga acatcgacca ggtggtcgaa ccctacattc
    17581 ggcccaaggc gcttcgttac ctcgaagaag gcaaggtcgt ggtgttcgcc gcgggcaccg
    17641 gcaacccgtt tttcacgacc gacaccgcgg cggcgttgcg cggggcggaa atcggagcgg
    17701 aaatcgtcct gaaagccacc aaggtcgacg gcatctacag cgccgacccc aacaaagacg
    17761 cctcggcgac gcgctactcg cgcatcagtt tcgacgaggc catcgtgcgc cgcctcgagg
    17821 tgatggacgc gaccgcgttc gcgctgtgca gagaccagaa gctgccggtc aaagtgtttt
    17881 ccatcaacaa gcccggtgcc ttgaaacggg tggtcgccgg cgaagacgaa ggcaccctgg
    17941 tccacgtttg aacataggaa caaccatgac cccctccgag atcaagaaat ccgctgaaga
    18001 ccgcatgaac aaatcgctcg aggcgctcca gacgggtttg tcgaagctgc gcactgggcg
    18061 tgcccacacc gggattctgg atcacgttca ggtcgaatac tatggctcgc cggtacctgt
    18121 gagccaagtg tcgaacgtca ccctgttgga cgcgcgcacc atcagcgtgc aggtctggga
    18181 aaagaacatg gccggtccgg ttgaaaaagc catccgcgag tccgatcttg gcctcaaccc
    18241 cattcccatg ggcgacaaca tccgcgtgcc tatgccggcg ctcaccgaag agcgtcggcg
    18301 cgacctcacc aaagtggcca ggggcgaagg cgaggaagcc aagatcgcca tccgcaacct
    18361 gcgccgtgag gcgaacgaca ccctgaagaa actggtcaag gaaaaagaaa tctccgagga
    18421 tcaggagcgc cgttcacagg aagaaatcca gaagctcacc gataaattcg tactcgacgt
    18481 cgacaagctg gtcgcccaga aagacgccga gatcatgaca gtctgagcgg ccccgcgctc
    18541 gacgcgaacc gcggacacca cacccaccat gcaagccagc tccacgcaga cgatcccgga
    18601 acgctccgac gtcccgcggc atctcgcgat catcatggat ggcaatggcc ggtgggcgac
    18661 gcagcgcttc cttccgcgca ccgccggcca tgcccgcggc gtgcaggccg tgcgccgcat
    18721 cgtcgaggcc tgtggccgtc ggggcgtgcg atacctcacc ctgttcgcct tcagctccga
    18781 gaactggcgc cgcccgcaag aggaagtgtc cttgctgatg cgcctgttca tccagacgct
    18841 cgagaaggaa gtcgggaaac tcgaagagca gggggtgcgt ctgcgcgtgg tcggtgacac
    18901 ctcggcgttc gaacccaggc tgcagtcgct catccgcctg gtcgaagagc gcacggcgca
    18961 caacgcattg ttgaatctga ccgtggccgc gaactatggc gggcgctggg acattctcca
    19021 ggcgatgcgt cgcatgctgg ccgccgagcc cgggctgtcc aatcagccgg acgacatcga
    19081 cgaagcgcga ctggcgcccc atttgtccat ggcctgggca cccgaacccg atctattcat
    19141 ccgcactggc ggagagcagc gcatctctaa ttttctggtg tggcaactgg cctatactga
    19201 actctacttc accgacagct actggcccga cttcggcgcc aaagagctcg acgccgcgtt
    19261 cgagtggtac cgcacccgcg agcggcgctt cggccgcac
//
LOCUS       DASTNK010000093         3547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_806141, whole genome shotgun sequence.
ACCESSION   DASTNK010000093 DASTNK010000000
VERSION     DASTNK010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806141"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(322..2031)
                     /locus_tag="VFR20_07225"
     CDS             complement(322..2031)
                     /locus_tag="VFR20_07225"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEU0230346.1"
                     /translation="MATHMIWFTLIRVIGWTLVHFLWQGTLLGLVYFSVRSILPRGTA
                     RYRFGMGILFALAACPLVTMWQLLDTVSLTSAAGQAPAMSLVVGEPVAGGAGHAVSGF
                     DVLLPWLVLAWSIGVLLHSVRACMQWRALKMLVREAEQLPQWQQRIADMAGRFALHRR
                     ITVLGSRIISSPVLIGWIRPVILLPMAVVCGFPASQVELILAHELAHLRRWDPLANLF
                     QVVLETVHFYHPVVRWISRDVNNEREICCDRLALALGGGSRREFVAVLAELGDLRMRQ
                     KSLVLAANGGVLLDRVQLMVLPQQQVGRLRKGGYTVALVLCAAMAMLTVRLQWIQAQM
                     NDGMNVAIRQLHELVMPAALPLMSPPSTWQVPNLLQIPSGAIRLVEAKVTADAPGAMA
                     FARPVDLGRPAAVSLVNLRSDAFDVLAMRPMEAIADPAVTVVTPTPVRVRQPVYPRRA
                     LSDGIEGRVVVEFALGRDGGVRDLRLVSSVPVGVFDQAALDAMRSWKYAVPAGVASTR
                     YRQILSFTLKSASAGRQDGQAAATENIQAKSVCQIPTGTHICRWPDNETTPDKVAPGA
                     VSR"
     gene            complement(2031..2426)
                     /locus_tag="VFR20_07230"
     CDS             complement(2031..2426)
                     /locus_tag="VFR20_07230"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEU0230347.1"
                     /translation="MKKIAEDMRKPTKAEMEILNVLWARDACTVREVHDVLRLGDRTG
                     YTTVLKLLQLMHAKGLVERDDSQRAHIYRAAASKKRTQKYLLMDLVQRVFDGSSSQLV
                     LQALGSQPTSREELDAIRKLLNQLDRESP"
     gene            complement(2542..3030)
                     /locus_tag="VFR20_07235"
     CDS             complement(2542..3030)
                     /locus_tag="VFR20_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230348.1"
                     /translation="MKLNGWMIGIIVAVALMQVGIVQASKTKVIVKAETRDDFVAVVA
                     AVHQQMQPGGQYEHVSASERASIDQHFNDMQGIFDKYGTVAQMDEKAKIRLFNDQEFV
                     NATLTKRDNKRLVCEHVAPVGSNIPRTSCRTYGDIMRSQRDTNRMLNDMRRVQETRGG
                     GG"
     gene            complement(3163..>3547)
                     /locus_tag="VFR20_07240"
     CDS             complement(3163..>3547)
                     /locus_tag="VFR20_07240"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HEU0230349.1"
                     /translation="FLVQEFIAEANGSDIRCFVVGRKVVAAMQREASPGDFRANLHRG
                     GSAVAANLSAEEKRLAVKAAAALGLGIAGVDLLRSRRGPLLLEVNASPGLEGIEAATG
                     VDVAGLIVELLQSRVRHLAGVAPPG"
BASE COUNT          697 a         1225 c         1019 g          606 t
ORIGIN      
        1 tcttcccggg gtctccaaga aaaaggccca gatgcacgac tcgccgtcgc ccacctggac
       61 ccaaacaact gccccgctac catcacctgc catccatcac gggctcaccc gccgacagac
      121 aacgcggaac ttatagcgag actcaggtta gatgccggtt aaccacctgg ccggcaggcc
      181 acgcgtcact ccgttacccg gtttctgcgc tctgccgatg acgagaacag ggggcgccgc
      241 cgcgcttccc tcgtggcaga ccggaaaggc gggcgaaatc cttcggggca cgcgggcgcc
      301 acatgacggg gaccgaccag gctatcggga gacggcgccg ggagcgactt tgtcgggcgt
      361 ggtttcattg tccggccaac ggcaaatatg cgtgcccgtg ggaatctggc acaccgactt
      421 ggcctggata ttctccgtgg cagccgcctg cccgtcttgg cggccggcac tcgcgctctt
      481 gagcgtaaac gagagaatct gtcgatagcg ggtcgacgcc acccccgccg gcacggcata
      541 tttccaggag cgcatggcgt ccagggcagc ctggtcgaat accccaaccg ggaccgagct
      601 gaccaggcgc aaatcgcgca cgccgccgtc cctgcccaag gcgaactcca ccacgacccg
      661 gccctcgatg ccatcgctga gcgcgcgccg gggatagacc ggctgacgga cccgcaccgg
      721 ggtcggcgta accacggtga ccgctgggtc ggcgatggcc tccatcggcc tcatcgccag
      781 cacgtcaaac gcgtccgacc tgagattgac cagcgaaacc gctgccggcc ggcccaaatc
      841 gacaggccga gcgaacgcca tggcaccggg agcatcagcg gtgacctttg cctcgaccag
      901 ccggatcgcc cctgacggga tctgcagaag attgggcacc tgccacgtcg aagggggact
      961 catcagaggc agggcggcag gcatcaccag ttcgtgcaac tgcctgatcg cgacattcat
     1021 gccgtcgttc atttgagcct ggatccattg caagcgcacg gtcagcatgg ccatcgccgc
     1081 acagagcacc agcgccaccg tatatccgcc cttgcgcaag cggccaacct gctgctgcgg
     1141 caacaccatc agttgcacgc gatccagcaa cacgccccca ttggccgcca gcaccaggct
     1201 cttctgccgc atacgcaggt caccgagttc ggccagcacc gcaacaaact cccgacgact
     1261 gccgccgccc agggccagag ccaaccggtc gcaacagatt tcgcgctcgt tgttgacgtc
     1321 ccgcgatatc caccgcacca caggatgata gaaatgcaca gtttccagca cgacctggaa
     1381 caggttggcc agcggatccc aacggcgcaa atgggccagt tcgtgagcca ggatcaactc
     1441 gacctgggac gcgggaaagc cgcacacgac ggccatgggc aggaggatca ccgggcgaat
     1501 ccagccgatc agcaccggcg aactgatgat gcggctgccc agtaccgtga tgcgccgatg
     1561 cagggcgaaa cgcccggcca tgtcggcaat ccgttgctgc cattgcggca actgctcggc
     1621 ctcgcgcacc agcatcttca acgcacgcca ctgcatgcag gcgcggaccg aatgcagcag
     1681 caccccgatc gaccaggcca gcaccagcca cggcagcaag acatcgaatc ccgaaaccgc
     1741 atgcccggcg ccgccagcga ccggttcacc gacgaccaac gacatggcgg gcgcttgacc
     1801 tgccgcggac gtcaaggaca cggtgtccag caattgccac atcgtgacca atggacacgc
     1861 ggccagcgcg aacagaatcc ccatcccgaa gcgatagcgt gccgtgccac gcggaaggat
     1921 cgatctcacg gaaaaataca ccaggccaag caaggtgcct tgccacagga aatgcaccag
     1981 agtccatcca atcacccgga tcagcgtgaa ccagatcatg tgcgtggcca tcacggcgat
     2041 tccctgtcga gctggttcag cagcttgcgg atcgcatcca gttcctcgcg gctggttggc
     2101 tggctgccca gggcctgcaa taccagctgc gaggacgagc cgtcgaatac ccgttgcaca
     2161 agatccatca agaggtattt ctgtgtgcgc ttcttgctgg ccgctgcacg gtatatgtgg
     2221 gcgcgctgcg agtcgtcccg ctccaccagc cccttggcgt gcatgagctg aagcagcttc
     2281 agcaccgtcg tgtaaccggt acgatcgccc aggcgcaata cgtcatgaac ctcacgaacc
     2341 gtgcaggcat cgcgcgccca caagacgttc aggatttcca tctcggcctt ggtcggcttc
     2401 cgcatgtcct cggcaatttt tttcatctga atgcatgctc cacacggggt cgtggtgaga
     2461 caggcagccg ctgatccgcg tgggaggcca gctgacatct ccttcgaaac tggcctcggg
     2521 caccggccgt tgacggatgc ctcagccgcc gccgcccctc gtttcctgaa ctcgcctcat
     2581 atcgttcaac attcggttcg tgtcccgttg cgaccgcatg atgtcgccgt aagtgcggca
     2641 gctggtccgg ggaatgtttg atcccaccgg cgcaacgtgc tcgcacacca agcgcttgtt
     2701 gtcgcgcttc gtaagcgttg cattcacgaa ctcctgatcg ttgaacagcc ggatcttcgc
     2761 cttctcgtcc atctgggcga ccgtgccgta tttgtcgaat atcccctgca tatcgttgaa
     2821 gtgctgatcg atgctggcac gctcactcgc gctgacatgc tcatattgac cgcctggctg
     2881 catctgttga tgtaccgcgg caaccaccgc cacgaagtca tccctggttt ccgccttcac
     2941 gatcaccttt gtcttggagg cttgcacgat accaacctgc atgagcgcaa cagcaacgat
     3001 gatgccgatc atccaaccgt tcagcttcat gtctttctct ccccaatcaa gccgcatccc
     3061 atggtgggtt gcccgtcgcg agatcgccct gtccgggatc atagaaaggc ccattcggaa
     3121 ctgtcaacga aaagtttcgt actaacgtcc tttgcggctg tcttagccgg gcggcgctac
     3181 tcccgccagg tgacggacgc gtgactgaag cagttccacg atcaatcccg ccacatccac
     3241 gccggtagcg gcctcgatcc cttccaagcc gggcgaggcg ttgacctcca gcagcagcgg
     3301 gccgcgccgg gagcgcagca ggtcgacgcc ggcgatgccc aggcccagcg cggcggcggc
     3361 cttgaccgcg aggcgcttct cctccgcgct caggttcgcc gccaccgcgc tgccgccgcg
     3421 gtgcaggttg gcgcggaaat cgcccgggct ggcctcgcgc tgcatcgctg caaccacttt
     3481 cctgccgacc acgaagcagc ggatgtcgct cccgttcgcc tcggcgatga actcctgcac
     3541 caggaaa
//
LOCUS       DASTNK010000094        17147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1177683, whole genome shotgun sequence.
ACCESSION   DASTNK010000094 DASTNK010000000
VERSION     DASTNK010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 17147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17147
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177683"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1208
                     /gene="dctP"
                     /locus_tag="VFR20_07245"
     CDS             186..1208
                     /gene="dctP"
                     /locus_tag="VFR20_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEU0230350.1"
                     /translation="MKNSKFRGLILAAALGGCVAMFGPPAVAAAPMVLKISHQFPANT
                     DFRDQVARKFAAEVEKRTNGAIKFQIYPGSSLFKAKQQFDAMANGSLDMSVYPLAYSG
                     GKLPATNITLMPALITSYKQAYNWENAPIGKWLEKYLSDHGVVLVSWIWQAGGVASRD
                     KPVIVPADVKGLKVRGAGKMIDLMLQAAGGAIASFPSNESYNAMQSGVVDAIWTSSAS
                     LMSFRMNEVAKFITTAQDHTFWYMFEPLLMSKATYDKLTPAQQKIVMEVGHSLQQFSL
                     EACKKDDDKVAALYGKSGDKVSTMNRKQFDLWKAVAEKSAYQDFAKHVKNGAKLLKMA
                     EAVPAD"
     gene            1391..1918
                     /locus_tag="VFR20_07250"
     CDS             1391..1918
                     /locus_tag="VFR20_07250"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEU0230351.1"
                     /translation="METRLWRGFGRVMGGVSTFCGYLTGGLIVVSATLITYEVIWRYY
                     LARPHTWSLEVNIFLLIGATFLASPFTQFKRGHVGTEVLDVVLPKHWIPWRVVLGDVL
                     SCAVCAFIGVSMTSYTIQAWNEGWTTDSTWAPPLWIPYALMAVGLWLTVLQCLIQIVD
                     QIGQLRGGKQGAHHE"
     gene            1911..3248
                     /locus_tag="VFR20_07255"
     CDS             1911..3248
                     /locus_tag="VFR20_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEU0230352.1"
                     /translation="MSELHSGLLFVVGTLALLLSGMSVAFALGVISLLFMVVVMPPSM
                     ILTLPETLYGGMDNFTLLAIPLFILMGEAIGRTRASVDLYESAYRWLSKLPGGLGIAN
                     VVGCAIFAGMCGSSTATAAAMGSMGIPEMKKRGYSGGLAGGLIAAGGTLGILIPPSIT
                     FILYGIVAEQSIGKLFIAGIVPGILLATLFALWVSYKSHRDGRITPVGVVGSTVAPQR
                     IQRESFTWRERFESLPRLAPFGIIIFIVMVALYGGFATPSEVAGVGAFATLVMVMVVY
                     RCYRWKDLGPILLGTTKESCMLMMIIAMSFLYSNVMSYLHVTQSATQWLVGLEMSRWE
                     FLFWIDVLVLVLGFFLPPVAIILFITPVLLPGLQAHGFDLVWFGVQMTILMEAGLIHP
                     PIGLNLFVILGIAPEISLRELLWGVLPFLILILLFIILISVFPQIVMWLPARM"
     gene            3518..4474
                     /locus_tag="VFR20_07260"
     CDS             3518..4474
                     /locus_tag="VFR20_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0230353.1"
                     /translation="MTALLFTALVTVWGFTWFAIKLQLGPVAPELSICYRFVLAAAVL
                     WALLAVMRRIRSAPWAGQGWFALLGLTLFSLNFLLIYSATQFIASGVVSVLFTTATLF
                     NAFHQWLFLRRAPSPRVMAGALCGIAGIALLFLQEFSQLGAGHDQLLGIGLAIAGTYV
                     FSLGNMVSLRAVRVAGDLPNAIVRGMTWGVVFLAAFAFLRGVPFVVPTTLPYLAGLVY
                     LAIPGSIIGFFAYLSLLQRVGPDRAAYATVLFPVLALAVSTVLENYVWTLAGLGGFAL
                     VLAGNLVIFARLPHKWRTRSRLESAPDAPPVGGQRVDGIDAR"
     gene            4571..5353
                     /locus_tag="VFR20_07265"
     CDS             4571..5353
                     /locus_tag="VFR20_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEU0230354.1"
                     /translation="MLKGKRAVITGSTSGIGMAIARALAGAGADVVINGFGDADAIEK
                     ERVGIEKEFGVKAYYLNADLMKERATRDFVDAAAKAMGGLDILVNNAGIQHTDLIENF
                     PIDKWNAIIALNLSAVFHGTAAALPHMKKQGWGRIINIASAHGLVASVNKSAYVAAKH
                     GVVGLTKVTALENAGNGVTCNAICPGWVRTPLVEKQIQALAASKSLSIDDAARDLLAE
                     KQPSLQFVTTEQLGGAAVFLASDAAAQMTGTTMSIDGGWTAR"
     gene            5426..6205
                     /gene="yaaA"
                     /locus_tag="VFR20_07270"
     CDS             5426..6205
                     /gene="yaaA"
                     /locus_tag="VFR20_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEU0230355.1"
                     /translation="MLLLLSPAKKLDYDSPVRTALHTQPLFVDQAAALIKTLKKKKAA
                     DVAALMKLSDALAELNVRRYADWKPSFDPSTARQAALAFNGDVYGGLDAPSLSDAQLR
                     WAQEHIAILSGLYGVLRPLDLMRPYRLEMGTALATTRGKNLYDYWGSSIAGYLNTRLA
                     DQAHPLVLNLASDEYFKSVDLKALKARVIQCVFQNFKSDAWKVISFNAKRARGLMARF
                     VIDHKIADPKRLRGFDAEGYAFAESVSTPDKLVFRRAESTR"
     gene            complement(6194..8455)
                     /locus_tag="VFR20_07275"
     CDS             complement(6194..8455)
                     /locus_tag="VFR20_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family membrane protein"
                     /protein_id="HEU0230356.1"
                     /translation="MAPRIPQLLRFFYSHYFYGGLRKSVGTLLPVILLGGVFNQYEAG
                     MIAAVGALCVGIIDQPGGPRRFRTNEMLGGIVLGAVTVLITGMASSSPLAIWLVVPAL
                     CFFLSMFTVFGRRGGLIGFACLLLMTLTMHTPLPPERVLWYSAYTVAGGAFYFLFSEA
                     ISRLMWHREEQQTLSVALFATADYIAARARFYDVHSDLDACYRQLIRTQSDMADKHQT
                     ARDMVLRELPKGRAGRGDHLRTVLLNVFVDMVNLLDNMVATHTDYATLRRELPDSDTL
                     LYAQDALLKISLGLGRIALNISRNKKIAQRGNIKAETRAFEYELEIYRKHGLDKDKPE
                     VYALLVQILRRLRNAGRMVEQMTEHMNKPRSTESIDARLDKSLTRFLTRQEVRFGMLT
                     SNLRLDSSHFRYAIRVTIAAVLGLTITAFVARFVAREDATPMIMAHSYWIILTILIVM
                     KPGFALTRQRNGWRLMGTLMGCAVAFALFAMTNNPDLLLAALTVSCIMGNSLIMLNYT
                     LASLFNTLFVLLAYHFLSPSVTLVIGERAADTLIGCTLALLCSYVLPWWEYNFMKPLA
                     QAAKTANQDYLAAGLRYAALNRAHRDSSTENPTPTDGDFQDADAVWRLARKNVLVAFG
                     NFASAFYRMMDEPVAQQRNVPEINVLLIQNHVLASQISASIPILANLERVPPNVGRAL
                     DAIQALLANQDATPPASVEIDGDLATLAYPLKQMMRAAQAISQNTRKLGWEPAGAATV
                     NDAGTTVPQPSTR"
     gene            8933..10648
                     /locus_tag="VFR20_07280"
     CDS             8933..10648
                     /locus_tag="VFR20_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019248169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HEU0230357.1"
                     /translation="MELNGADIVVRCLIEEGVEHVFGYPGGAVLYIYDAIYKQDKFQH
                     VLVRHEQAAVHAADAYSRSSDKVGVALVTSGPGLTNAVTGIATAYMDSIPMVVISGQV
                     PSHAIGEDAFQECDTVGITRPCVKHNFLVRDVKDLAETMRRAFYIARTGRPGPVLVDI
                     PKDISMHVCKYTPPRGEVHMRSYAPVVKGHQGQIKKAVQMLAAAERPMIYAGGGVILS
                     NASAELRELVELSGAPCTNTLMGLGGFPASDAKFMGMPGMHGTYEANMAMQNCDVLIA
                     VGARFDDRVIGNPKHFSATPRKIVHIDIDPSSISKRVRVDVPIVGNVRDVLQDLITLY
                     RQSVQPASRPSLEGWWKQIEGWRAKDCLKYDHSGDIIKPQFVVQKLWEVTNGDAFVTS
                     DVGQHQMWAAKYYRFDEPRRWINSGGLGTMGVGLPYAMGVQMANPDRPVAVITGEASI
                     QMNIQELSTCSQYRLTPKILCLNNRYLGMVRQWQQIDYGSRYSESYVDALPDFVKLVE
                     SYGHVGLRIEKPADVEPAIREAFKKHKDRLVFMDFITDQTENVWPMVKAGKGLTEMLL
                     GSEDL"
     gene            10660..11151
                     /gene="ilvN"
                     /locus_tag="VFR20_07285"
     CDS             10660..11151
                     /gene="ilvN"
                     /locus_tag="VFR20_07285"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU0230358.1"
                     /translation="MKHVISILLENEPGALSRVVGLFSARGYNIETLTVAPTEDATLS
                     RMTIVTTGSDEIIEQITKHLNRLVDVVKVVDLSEGPHVERELMLIKVRAVGKERDEMK
                     RMAEIFRGRIIDVTEKVYTIELTGVQEKIQAFIDALDRSAILETVRTGVSGIGRGDRV
                     LKL"
     gene            11313..12329
                     /gene="ilvC"
                     /locus_tag="VFR20_07290"
     CDS             11313..12329
                     /gene="ilvC"
                     /locus_tag="VFR20_07290"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU0230359.1"
                     /translation="MKVFYDKDCDLSLIKGKTVAIIGYGSQGHAHALNLHESGVKVVV
                     GLRKGGASWNKAVNAGLQVKEVPEAVRGADVVMMLLPDEHIADVYRRHIAENIKAGAA
                     LAFAHGFNIHYGQVMPRNDIDVIMIAPKAPGHTVRSTYTQGGGVPSLVAIHQDKSGAA
                     RDVALSYACAIGSGRAGVIETSFREETETDLFGEQAVLCGGTVELIKAGFETLVEAGY
                     APEMAYFECLHELKLIVDLIYEGGIANMNYSISNNAEYGEYESGPKVIGDESKQAMRK
                     LLADIQSGVYAKSFILENAAGAPTLISRRRINAESQIEQVGGKLRAMMPWIAANKLVD
                     QTKN"
     gene            12514..13167
                     /locus_tag="VFR20_07295"
                     /pseudo
     CDS             12514..13167
                     /locus_tag="VFR20_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
     gene            13272..14120
                     /gene="pssA"
                     /locus_tag="VFR20_07300"
     CDS             13272..14120
                     /gene="pssA"
                     /locus_tag="VFR20_07300"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEU0230360.1"
                     /translation="MNLSSEETRRRRSIYLLPNAFTTANLFAGFYAVVQAMNGRFEIA
                     AMAIFAAMVFDGMDGRVARMTHTQSAFGEQYDSLADMASFGIAPALVIYEWMLRGLGR
                     WGWLAAFIYVVGAALRLARFNTNIGVVDKRFFQGLPSPSAAALVAGFVWLAVDNKFGM
                     YGGSLAWTAFVVTIYAGVAMVTNLPFYSGKSFALGRSVPFWVILVVVAVFVFVSSDPP
                     VVLFGLFVIYGVSGWGIWLWRWQRARKLNRLRQEGPEIPPVARPADAGSEKRAASFSV
                     GPRDDA"
     gene            complement(14228..14746)
                     /locus_tag="VFR20_07305"
     CDS             complement(14228..14746)
                     /locus_tag="VFR20_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230361.1"
                     /translation="MIRINRRIARCALAAASALALGACANMANTPPGTPIAQIEAKFG
                     APNFSCPLPNGGKRLIWTQQPAGQYAWGTNVDAAGDTDRVVPLLTDAHFKILAKGTWT
                     PQQVRCEFGPPAIKDTVGLPSDTQIVWSYRYKQDQVWNSFMYVYFGRDGSHVTRFHPG
                     PDLSYEDHFPFF"
     gene            14896..15165
                     /gene="rpsO"
                     /locus_tag="VFR20_07310"
     CDS             14896..15165
                     /gene="rpsO"
                     /locus_tag="VFR20_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU0230362.1"
                     /translation="MSVADINKSDIVAQYQRAQGDTGSPEVQVALLTARINELTDHFK
                     THLKDHHSRRGLLRMVSRRRKLLDYLKGRNPDSYRSLIEKLGLRK"
     gene            15255..>17147
                     /gene="pnp"
                     /locus_tag="VFR20_07315"
     CDS             15255..>17147
                     /gene="pnp"
                     /locus_tag="VFR20_07315"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU0230363.1"
                     /translation="MFNKVTKSFQYGQHTVVLETGEIARQASGAVMVSVDDTVILASV
                     VAAKKAKPGQDFFPLTVDYVEKTYAAGRIPGGFFKREGKPSEKETLTSRLIDRPLRPL
                     FPEGFYNDVQVIIHTMSVNPEVDPDIPAMIGASAALAISGIPFNGPIGAARVAYVNDQ
                     YVVNPTTTQLHDSKLNLVVAGTEAAVLMVESEARQLPEDVMLGAVVFGHEQMQVVINA
                     IHDLVAEAGKPEWDWQPAPKNEALVAAVTAAAQEGLHGAYQIREKQARTLRLREVYAS
                     VKDKLLEGATARGEEAPDSVEVENILFDLEAALVRSQILNGEPRIDGRDTRTVRPISV
                     RLGVLPRAHGSALFTRGETQALVVATLGTKQDEQIIDSIMGEYRDRFMFHYNMPPYAT
                     GETGRFGVPKRREIGHGRLAKRALLALLPDHQDFQYTIRVVSEITESNGSSSMASVCG
                     GSLAMMDAGVPIGDHVAGVAMGLIKEGGKFAVLTDILGDEDHLGDMDFKVAGTVQGIT
                     ALQMDIKIQGITKEIMQVALAQAREGRLHILGKMKDALEGSRTELSEFAPRMLSIKIN
                     PEKIRDVIGKGGATIRALTEETGTQIDINDDGTITIASADLDRAREAERRIKELTADV
                     EVGQEYE"
BASE COUNT         3165 a         5210 c         5420 g         3352 t
ORIGIN      
        1 gatcagtggg aggtattgcc ttgcggattt ccggtggttc aagtagtagt gcagtatttg
       61 acgctctctg gaggagaacg tgacagaagt gtttgtgcgg tatcgaaacc cagggttggc
      121 cggtcaatgc aataacaaac ctcatcggca tcccgtttac tgaccagtgg tcaggaggag
      181 acatcatgaa gaacagcaaa tttcgtggct tgattctggc ggccgcccta ggtgggtgcg
      241 tggcgatgtt cggcccgccg gccgtcgcgg ccgcgcccat ggtattgaag atttcccatc
      301 aattccccgc caacaccgat ttccgcgacc aggtggcgcg caagttcgcc gccgaggtcg
      361 agaaacgcac caacggggcg atcaagttcc agatctaccc ggggtcgtcc ctgttcaaag
      421 ccaaacaaca gttcgatgcg atggccaatg gctcattgga catgtcggtg tatccgctgg
      481 cctattccgg cggcaagctg ccggccacca acatcacgtt gatgccggcc ctgatcacca
      541 gctacaagca agcgtacaac tgggagaacg cgccgatcgg gaaatggctg gaaaagtatc
      601 tgtccgacca tggggtggtg ctggtcagct ggatctggca ggcggggggc gtggcctcgc
      661 gcgacaagcc ggtcatcgtg ccggccgacg tcaagggctt gaaggtgcgt ggcgccggca
      721 agatgatcga tctcatgctg caggccgcgg gcggcgcgat cgcgtccttc ccgtccaacg
      781 agtcgtacaa cgccatgcag tcaggcgtgg tcgacgccat ctggacgtcc agcgcgtcgc
      841 tcatgagctt ccgcatgaac gaagtcgcca agttcatcac cacggcgcag gaccacacct
      901 tctggtacat gttcgagccc ttgctgatgt ccaaggcaac gtacgacaag ctcacgcccg
      961 cgcaacagaa gatcgtgatg gaggtgggcc attcgctgca gcagttctcg ctggaggcct
     1021 gcaagaagga cgacgacaaa gtggccgctc tctacggcaa gtcgggtgac aaggtttcca
     1081 ccatgaaccg caagcagttc gacttgtgga aggccgtggc tgaaaagagc gcgtaccagg
     1141 acttcgccaa gcatgtgaag aatggcgcga agctgctgaa gatggctgaa gccgttcccg
     1201 ccgattgagt cggcatgacg tcggggtgcc gcccggttgg ttcgagcatt caaatccggg
     1261 tgcgcgaacc aacccgacaa cgcacaagga atcctctggc aagtcgaact gcaagcaggc
     1321 gcagcggctt gttcagagga tacctgttca gcggtttctg ttgatgcgtc gatttttatg
     1381 agaagaaagt atggagacaa gactgtggcg cggcttcggc cgggtgatgg gcggggtcag
     1441 cacgttctgc gggtacctga ccggcgggct gatcgtggtg tccgcgaccc tcatcacgta
     1501 tgaggtgatc tggcgctatt acctggcgcg gccccatacc tggtcgctgg aagtcaatat
     1561 tttcctcctg atcggagcca cgttcctggc ttcgccgttc acgcagttca agcgcggcca
     1621 cgtcggcacg gaggtgctcg acgtggtgct gcccaagcac tggatcccct ggcgggtagt
     1681 gctgggcgac gtgctgtcgt gcgcggtctg cgccttcatc ggcgtcagca tgacgtcgta
     1741 tacgatccag gcgtggaacg agggttggac cacggattcc acctgggcgc cgcccttgtg
     1801 gattccctat gcgttgatgg cggtggggtt gtggctgacc gtgttgcagt gcctgatcca
     1861 gatcgtcgat cagatcggac agcttcgcgg tgggaagcaa ggggcgcatc atgagtgaac
     1921 tccattccgg acttctgttc gtggtcggca cgctggcctt gctgctgtcg ggcatgtcgg
     1981 tggccttcgc cctgggcgtg atctcgctct tgttcatggt ggtcgtcatg ccgccttcca
     2041 tgatcctcac cctccctgaa acgctgtacg ggggcatgga taacttcacg ctgttggcga
     2101 ttcccttgtt cattctcatg ggcgaggcca taggccgaac gcgcgcctcg gtggaccttt
     2161 acgagtcggc ctatcgctgg ctcagcaagc tgccaggggg gttggggatc gccaacgtgg
     2221 tcgggtgcgc gatcttcgcc ggaatgtgcg gctcgagcac cgccacggcg gctgccatgg
     2281 ggtccatggg catccccgag atgaagaagc gcgggtattc cggcgggctt gccggtgggc
     2341 tgatcgccgc cggcggcacg ctgggcatcc tgattccacc atccatcact ttcattctgt
     2401 acgggatcgt ggccgagcag tccatcggga aactgttcat cgcggggatc gtgccaggca
     2461 ttctgctcgc tacgttgttc gcgctgtggg tgagctacaa gtcgcaccgc gacggcagga
     2521 tcaccccagt cggcgttgtg ggctcgacgg tcgcaccgca gcgcattcag cgggagagct
     2581 tcacctggcg cgagcgtttc gaatcgttgc cgcgcctggc gccattcggg atcatcatct
     2641 tcatcgtgat ggtcgcgctg tatggcgggt tcgccacgcc gtccgaggtg gcgggggtgg
     2701 gcgcgttcgc aacgctggtg atggtgatgg tggtgtatcg ttgctatcgc tggaaagatc
     2761 tgggcccgat cctgcttggc acgaccaagg aatcgtgcat gctcatgatg atcatcgcga
     2821 tgtcgttcct gtattcgaac gtgatgagct atctgcacgt cacacagagc gccacgcaat
     2881 ggctggtcgg actcgagatg tcgcgctggg agttcctgtt ctggatcgat gtgctggtgc
     2941 tggtcctggg tttcttcctg ccaccagtgg cgatcatcct gttcatcaca ccggtgttgc
     3001 tgccggggct gcaagcgcat ggcttcgacc tggtctggtt cggcgtgcag atgaccatcc
     3061 tgatggaggc cgggctgatt catccgccga tcggactcaa tctgttcgtg atcctgggca
     3121 tcgcaccaga gatttcccta agggaattgt tgtggggggt gctgccgttc ctcattctga
     3181 tcttgttgtt catcatcctg atctcggtgt ttccgcagat tgtgatgtgg ctgcccgcgc
     3241 gaatgtaggg gagtcgccgc agtgtggaac cgagtcgcgg agccatggcg gacatggctc
     3301 cgcgacgttg cctggatggt gcgaataccc tgctttgaca acaaaattcc gcaaatctgt
     3361 gacagaccgg agatcgcgtc gcgttctata gtgggccgag actcctctga gcaagccgcc
     3421 gcgccgcctg cttcggggct tgaggcggtc cgccgttcga cttgtccagg gaatccccgg
     3481 ttttttcctg tcacccactt ttgctgcgat tttcatcatg actgcgcttc ttttcacggc
     3541 cctggtcacg gtctgggggt tcacctggtt cgcgatcaag cttcagctcg ggcctgtggc
     3601 gcccgaattg tccatctgct atcgcttcgt gctggcggcg gcggtcttgt gggcactgtt
     3661 ggcggtgatg cgccgcattc gttccgcgcc ctgggcgggc cagggctggt tcgcgttgct
     3721 ggggctgacc ctgttcagcc tgaacttcct gctgatctat tcggcgaccc agttcatcgc
     3781 cagcggcgtg gtgtcggtgc tgttcaccac ggccacgctg ttcaacgcct tccaccagtg
     3841 gctgttcctg cggcgcgcgc ccagtccacg tgtcatggcg ggggccttgt gcggcatcgc
     3901 cggcatcgcc ttgctgttcc tgcaggagtt ctcgcagctg ggagcggggc acgaccagtt
     3961 gctcggcata gggctggcca tcgcgggcac gtacgtgttc tcgctcggga acatggtgtc
     4021 ccttcgcgcg gtgcgggtcg cgggcgatct gcccaatgcc atcgtgcggg gcatgacctg
     4081 gggcgtcgtg ttcctggccg cgttcgcctt ccttcgcggg gtgccgttcg tcgtgccgac
     4141 cacgctgccg tacctggctg gcctggtcta tctggccatt cccggctcca tcatcgggtt
     4201 tttcgcctac ctgtccctgc tgcagcgcgt gggacccgac cgtgccgcgt atgccacggt
     4261 gctgttcccg gtgctggcgc tggccgtgtc cacggtgctg gaaaattacg tgtggacgct
     4321 ggcgggtttg ggcgggtttg cgctcgtgct ggcggggaat ctggtgattt tcgcgcgcct
     4381 tccccacaaa tggcgtactc gtagccggct cgaaagcgcg cccgacgccc cgcccgttgg
     4441 tggccaacga gtcgatggca tcgacgcgcg ttaaactcaa cgttgtccaa cccggagccc
     4501 gttccctttc agggggaacg ggctcaagag cttgatcggg tgttttcaac gttacgtgaa
     4561 agggaatgac atgctcaaag gcaaacgcgc agtgatcacc ggctccacca gcggcatcgg
     4621 catggcgatc gcccgcgcat tggcgggtgc cggcgccgac gtggtcatca acggcttcgg
     4681 cgatgcggac gccatcgaaa aggaacgcgt cggcatcgaa aaggaattcg gcgtcaaggc
     4741 gtattacctg aatgccgatc tgatgaaaga gcgggccacg cgcgacttcg tcgatgcggc
     4801 ggccaaggcg atgggcggtc tggacatcct ggtgaacaac gccggcatcc agcacacgga
     4861 cctgatcgag aacttcccca tcgacaaatg gaacgcgatc atcgcgttga acctgtcggc
     4921 ggttttccac ggcacggccg cggcgctgcc gcacatgaaa aagcagggct gggggcgcat
     4981 catcaacatc gcctcggccc atgggttggt ggcatcggtc aacaagtcgg cctatgtggc
     5041 cgccaagcat ggcgtggtgg gcctgaccaa ggtcacggcc ctggagaacg ccggtaatgg
     5101 cgtcacgtgc aacgcgattt gcccgggatg ggtgcgcacg ccgctggtcg agaaacagat
     5161 ccaggcgctg gccgcaagca agagtctttc catcgatgac gccgcgcgtg atctgctggc
     5221 cgagaagcag ccatcactgc agttcgtcac gaccgagcaa cttggcggtg cggccgtgtt
     5281 cctggcatcg gacgcggcgg cgcagatgac cggcaccacg atgtccatcg acggtggttg
     5341 gacggcccgg taacgcgaca atcctgtaac gttcctcatt actcccatac ccggtaacgt
     5401 cggttacgct ctaaagaggt tcttcatgtt gttgctgttg tcgccggcca agaagctgga
     5461 ctacgattcg cccgtgcgca ccgcgctgca cacacaaccg ctgttcgtgg accaggccgc
     5521 ggccttgatc aagacgttga agaaaaagaa ggccgccgac gtcgccgcgc tgatgaaact
     5581 cagcgacgcg ctggccgaac tcaacgtgcg gcgctacgcc gactggaaac catcgttcga
     5641 tccgtccact gcgcggcagg cggcgctggc gttcaacggc gacgtctatg gcggcctcga
     5701 cgcgcccagt ctgtcggacg cgcaattacg ttgggcgcag gaacacatcg ccatcctcag
     5761 cggcctgtat ggtgttctgc ggccgctcga cctgatgcgt ccctaccgtc tcgagatggg
     5821 cacggcgctg gccaccaccc gggggaagaa cctgtacgac tactgggggt cgtcgatcgc
     5881 cggctatctg aacacccgcc tggcggatca ggcccatccc ctggtcttga acctggcgtc
     5941 cgacgaatac ttcaagtcgg tcgacttgaa ggcgctgaag gcgcgtgtca tacagtgcgt
     6001 gttccagaac ttcaagagcg atgcgtggaa ggtgatcagc ttcaatgcca aacgggcgcg
     6061 gggcctgatg gcccgtttcg tcatcgacca caagatcgcc gaccccaagc ggctgcgcgg
     6121 gttcgacgcc gagggctatg cgtttgccga gtcggtgtcc acccccgaca agctggtgtt
     6181 ccggcgggcc gaatcaacgc gttgagggct ggggcacagt ggtgccggcg tcgttgacgg
     6241 ttgccgcgcc cgcaggctcc cagcccagct tgcgggtgtt ctgggatatc gcctgcgccg
     6301 cccgcatcat ctgcttcagc ggataggcca gggtggccag gtcgccgtct atttccacag
     6361 aggccggcgg cgtggcgtct tgattcgcca agagagcctg gatggcgtcc agggccctgc
     6421 ccacgttggg cggaacgcgt tccagattgg ccaggatcgg aatcgacgcg ctgatctgtg
     6481 aagccagtac gtggttctgg atgagcaaaa cattgatctc gggcacgttg cgctgctgcg
     6541 cgacgggttc gtccatcatc cggtagaacg ccgaagcgaa gtttccgaaa gccaccagta
     6601 cgttcttgcg cgccaggcgc cacaccgcgt cggcatcctg gaaatcgccg tcggtggggg
     6661 ttgggttttc cgtcgatgaa tcgcgatgcg cccgattcaa tgcggcgtag cgcaggcccg
     6721 ccgccaggta gtcctgattg gcggtcttgg cggcctgcgc cagtggtttc atgaagttgt
     6781 actcccacca cggcagcacg tagctgcaca gcagcgccaa tgtgcagccg atgagagtgt
     6841 cggcggcgcg ttccccgatc accagcgtga ccgacggcga caggaaatga taggccagca
     6901 gcacgaacag tgtgttgaac agcgaagcca gggtgtagtt cagcattatc aggctgttgc
     6961 ccatgatgca cgacacagtc agggcagcca gcaggagatc ggggttgttg gtcatggcga
     7021 acaacgcgaa tgccacggcg catcccatca gtgtccccat gaggcgccag ccgttgcgct
     7081 gccgcgtcag cgcgaatccg ggtttcatga cgatcagaat ggtcaggatg atccagtagc
     7141 tgtgggccat aatcatgggc gtcgcatctt cgcgcgcaac gaaccgggcc acgaacgcgg
     7201 ttatcgtcag gcccagcaca gccgcgatgg tgacgcggat ggcgtagcgg aaatgcgacg
     7261 aatccaggcg cagattgctg gtgagcatgc cgaaacgcac ttcctggcgt gtcaggaagc
     7321 gggtcagcga cttgtcgagc cgggcatcta ttgattccgt ggatcggggc ttgttcatgt
     7381 gctcggtcat ctgctcgacc atgcgtccgg cgttgcgcag acggcgcaga atctgcacca
     7441 gcagggcata gacttcgggc ttgtccttat ccaggccatg cttgcggtag atttcgagct
     7501 cgtactcgaa ggcgcgggtc tcggccttga tgttgccgcg ttgcgcgatc ttcttgttgc
     7561 gcgagatatt cagcgcaatg cggcccaggc ccaatgaaat cttgagcagg gcatcctggg
     7621 catagagcag cgtgtcgctg tcggggagtt cgcggcgcag ggtggcatag tcggtatgtg
     7681 tggccaccat gttgtcgagc aagttgacca tgtcgacgaa cacattgagc agcaccgtgc
     7741 gcaggtgatc gccacgccct gcgcgcccct tgggcagttc gcgcaacacc atgtcgcgcg
     7801 cggtctggtg cttgtccgcc atgtccgact gggtgcgtat aagttggcgg tagcaggcat
     7861 ccaggtcgga atgcacgtcg tagaaccgcg cccgcgcggc gatgtaatcg gcggtggcga
     7921 acagcgcgac cgacaatgtc tgctgctctt cccggtgcca catcagccgc gagatggctt
     7981 cactgaacag gaagtagaac gcgccgcccg ccacggtgta ggcgctgtac cacagcacgc
     8041 gttcaggcgg caggggtgtg tgcatggtga gcgtcatcaa cagcaggcag gcgaagccga
     8101 tcaggccgcc acgcctgccg aacaccgtga acatggaaag gaagaaacac agggccggca
     8161 ccacgagcca gatggccaac ggactggacg aggccatgcc ggtgatgagc accgtgaccg
     8221 cgccaagcac gatgccgccc agcatctcgt tggtgcggaa gcgccgcggg ccgcccggct
     8281 ggtcgatgat gcccacgcac agcgcgccca cggcggcgat catgccggct tcatattgat
     8341 tgaaaacgcc gcccagcaaa atcactggca gcagggttcc cacggatttg cgcagcccgc
     8401 catagaaata atggctatag aagaatcgaa gcagttgcgg tatgcgtggg gccatggcgg
     8461 attggggtga cgggtgcctt ggtgggccac gatgggctcg gggcaccggg ccgcgcagcg
     8521 ggcgccgggc gtgttacgca caataatacc gcatggcccg cggcatcaag cggggcgggg
     8581 cgtcggttgc atccgatggt gcgacgcggt aaagtatgcc atcggtcggc cggagcacag
     8641 ggctggccgc caaaggctct ttcgcccggg ctatccgccc ggttttttgc gcccgtatca
     8701 cctgccgctg gccgtgccac aagcgtgtgt tgccaaggct tccgtttcta actcccgcgt
     8761 tcgtcgcgaa cgtgcgtatc gtgtttcaac gagtacgtgc cggctcgaca aggtgtccgg
     8821 gtggcgtggc cttgtgggcc acaaagcgcc ggatcatgtt gtcctgaccg tcccgtctcg
     8881 ggctggattc agccagttga agcgtcggta ccagttgtga aaggaataca acatggaact
     8941 caatggcgcc gacatcgtcg tccgctgcct catagaagaa ggggtcgaac atgtgttcgg
     9001 ctatcctggc ggcgcggtgc tctacatcta cgacgcaatc tacaagcaag acaaattcca
     9061 gcatgttctg gtccggcacg agcaggccgc tgtgcacgcg gctgacgcgt attcgcgctc
     9121 gtccgacaag gtcggcgtgg ccctcgtcac cagtggtccg gggctgacca acgcggtcac
     9181 cggcatcgcc acggcgtaca tggactcgat ccccatggtc gtcatcagcg ggcaggtgcc
     9241 cagccacgcc atcggcgaag acgctttcca ggaatgcgac acggtcggca tcacgcggcc
     9301 atgtgtcaag cacaacttcc tggtgcgcga cgtcaaggat ctggccgaaa ccatgcgccg
     9361 ggccttctac atcgcgcgca cgggccggcc cggcccggtg ctggtcgata ttcccaaaga
     9421 catttccatg cacgtctgca agtacacgcc gccgcgcggg gaagtgcaca tgcgctcgta
     9481 tgcgccggtg gtcaaggggc accaagggca aatcaagaaa gccgtgcaga tgctggctgc
     9541 ggccgagcgt cccatgatct atgcgggcgg gggcgtgatt ctgtcgaatg cttcggctga
     9601 actgcgtgaa ctggtcgaac tatccggcgc gccctgcacg aacaccctga tggggctggg
     9661 cgggtttccc gccagtgatg cgaagttcat ggggatgccg ggcatgcatg gcacgtacga
     9721 agccaacatg gccatgcaga actgcgatgt cctcatcgct gtcggcgcgc gcttcgacga
     9781 ccgcgtcatc ggcaacccca agcacttctc ggcgacgccg cgcaagatcg tgcatatcga
     9841 catcgacccg tcgtccatct ccaagcgggt gcgcgtcgac gtgcccatcg tgggcaatgt
     9901 gcgcgatgtc ctgcaggatc tcatcacgct ctatcgccag tcggtccagc cggcctcccg
     9961 gccttccctg gaaggatggt ggaagcagat cgagggctgg cgcgcgaagg attgcctgaa
    10021 atacgatcac tccggcgaca tcatcaagcc tcagttcgtg gtgcaaaagc tgtgggaagt
    10081 taccaatggc gatgccttcg tgacgtccga cgtaggccag caccagatgt gggcggccaa
    10141 gtattaccgc ttcgatgaac cccggcgctg gatcaattct ggcggtctcg gcaccatggg
    10201 tgtgggcctg ccctatgcca tgggtgtgca gatggccaac cccgaccggc cggtggccgt
    10261 catcacgggc gaggcgtcca tccagatgaa catccaggaa ctgtccacct gcagtcagta
    10321 ccgcctgacg cccaagatac tgtgcttgaa caaccggtat ctcggcatgg tgcgccagtg
    10381 gcagcagatc gattacggtt cgcgctattc cgaatcgtac gtcgacgcgc tgcccgactt
    10441 cgtcaagctg gtggaaagct atggccacgt cggtctgcgc atcgaaaagc ccgccgatgt
    10501 ggaaccggcg atccgggagg cgttcaagaa gcacaaagac cggctggtgt tcatggattt
    10561 catcaccgac cagaccgaaa acgtctggcc catggtgaaa gcggggaagg gcttgactga
    10621 aatgttgctc ggctcggaag atctataagg ataacgacca tgaaacatgt catctccatt
    10681 cttctggaaa acgagccggg cgcgttgtcg cgcgtggtcg ggctgttctc ggcgcggggc
    10741 tacaacatcg aaaccctcac cgtggcgccc accgaagacg cgacgctttc ccgcatgacc
    10801 atcgtcacca cggggtcgga cgaaatcatc gagcagatca ccaagcacct gaaccgcctg
    10861 gtcgacgtgg tcaaggtggt cgatctctcc gagggacccc atgtcgagcg cgagctcatg
    10921 ctgatcaagg ttcgcgcggt cggcaaagag cgtgacgaaa tgaagcgcat ggccgagatc
    10981 ttccgcggcc gcatcatcga cgtcaccgaa aaggtctaca cgatcgagct caccggcgtg
    11041 caggaaaaga tccaggcatt catcgatgcc ctcgatcgca gtgccatcct cgaaaccgtg
    11101 cgcacgggcg tgtcgggcat tggtcgtggc gatcgcgtct tgaagctcta ggtttttccg
    11161 taagccggcc ctggcgcggc caccgcgccg ggtctggcct atgatgcagg tttttcagta
    11221 cccgttttcc cgtatccatt cgaagcacac tacccattcg aagcactttt caacaatttg
    11281 atcatccgca taaagcgact ggagccctac aaatgaaagt tttctatgac aaagattgcg
    11341 acctgtccct catcaagggc aagaccgtgg ccatcattgg gtatggttcg caaggccatg
    11401 cccacgcgct gaacctgcat gaatccggcg tcaaggtcgt ggtcggcctg cgcaaaggcg
    11461 gggcatcctg gaacaaggcc gtcaacgccg gcctgcaggt caaggaagta cccgaggccg
    11521 ttcgtggcgc cgacgtggtc atgatgctgc tgcccgacga gcacatcgcc gacgtctatc
    11581 gccggcacat cgccgagaac atcaaggccg gcgccgccct ggctttcgcg catggcttca
    11641 acatccacta cggccaagtc atgccgcgca acgacatcga cgtgatcatg atcgcgccca
    11701 aggcgcccgg ccacactgtg cgcagcacct acacccaggg tggcggcgtg ccgtcgctgg
    11761 tggcgatcca tcaagacaag tccggcgccg cgcgcgacgt ggcgttgtcg tacgcttgcg
    11821 ccatcggcag cggccgggca ggcgtcattg aaaccagctt ccgcgaagaa accgaaacag
    11881 acttgttcgg cgaacaggcc gtgctgtgtg gtggcaccgt cgagctcatc aaggccggtt
    11941 tcgaaacact ggtcgaagct ggctatgcgc cggaaatggc ctatttcgaa tgcttgcacg
    12001 aactcaagct catcgttgac ctcatctacg agggcggcat cgccaacatg aactactcca
    12061 tctccaacaa tgcggagtac ggcgaatacg aaagcggccc caaggtcatt ggcgacgaaa
    12121 gcaagcaggc catgcgcaag ctgctggccg atatccagtc gggtgtctac gccaagagtt
    12181 tcattctcga gaacgccgct ggtgccccca ccttgatttc gcgtcgccgc atcaatgctg
    12241 aatcgcagat cgagcaggtc ggtggcaagc tgcgcgccat gatgccctgg attgccgcca
    12301 acaagctggt cgaccagacc aagaactaac ccggtaaaag ggttcggcaa cggtggccaa
    12361 gccaccgttg cctggcatgt ggcgcggcat tgcactctgt gccgatctgc atggcgtctc
    12421 tggctgccag ctggcgcgac cgcatcgcac ttttgattag gcgacgcagg tcgctgaaag
    12481 ggcgggggca tttcggttat ccttcaccct acgatgaata aacctgcgta tccgcatccc
    12541 ctcatagcgc gcgaaggctg gccgtttctt gccggcatcg tgatcatttc ctttctggtc
    12601 tggctctggt ccggctgggc gtccctgccg ttgtggctgc tgtcgctgtt cgtgctgcag
    12661 ttcttccgcg acccggcgcg catcgcgccg gatggcgaat tcaacgtgct gtcgcccgcc
    12721 gatggccgcg tggtcgcggt cgaggaaacc cgcgacgcct atgccgatgg tcgctcggcg
    12781 cttaaaatta gcgtgttcat gaacgtgttc aacgtccact cgaaccgttc tccggtgcac
    12841 ggcactgttg ccagtctggc ttacttcccg ggcaagtttt tcaatgcggc actggaaaag
    12901 gcctcgctcg aaaacgaacg caacgccatg gtcattcgca ccgatcacgg tcacacggtc
    12961 accgccgtgc aggtggccgg tctggtggcc aagcgcatcc tttgctatgt caagccaaac
    13021 gacccgctct acggtggcca gcgctatggc ttcatccgtt ttggttcccg ggtcgatgtc
    13081 tacctgccct tggggtcgcg cgtccgcgtg gccatcggcg acaaggtcca ggccaccagc
    13141 accgtgctgg ccgaattgcc tgaacagacc agtccgagcc agaacagcct gttcgatggc
    13201 gatcaagact ccggcagtca aagtccaggt agccaggccc caagcggcag tcaggacacg
    13261 caatcgtgac aatgaatctc tcctctgaag aaacccggcg ccggcgcagc atctatctgc
    13321 tgccgaacgc cttcaccaca gccaatctgt ttgccggctt ctatgccgtg gtgcaggcca
    13381 tgaacggccg tttcgaaatc gccgcgatgg ccatcttcgc cgccatggtg tttgacggca
    13441 tggatgggcg cgtggcgcgc atgacgcaca cgcagtcggc tttcggtgaa cagtacgact
    13501 ccctggccga catggcctcg ttcggcatcg cgccggcgct ggtcatttat gagtggatgc
    13561 tgcgcggtct gggtcgctgg ggctggctgg cggcgttcat ctacgtcgtg ggcgcggcgc
    13621 tgcgtctggc gcggttcaac accaacatcg gcgtggtcga caagcgcttc ttccaggggc
    13681 tgcccagccc gtcggcggcg gcgctggtcg ccggcttcgt ctggctggcg gtggacaaca
    13741 agttcggcat gtacgggggt tcgctcgcct ggacggcgtt cgtcgtgacc atttacgcgg
    13801 gcgtcgccat ggtcactaat ctcccgttct acagcggaaa atcgttcgct ctggggcgca
    13861 gtgtgccgtt ctgggtgatt ctggtcgtgg tcgccgtttt tgtgttcgtg tccagcgacc
    13921 cccccgtggt gctgtttggt ctgttcgtga tctacggcgt atcggggtgg ggcatctggc
    13981 tatggcgctg gcaacgcgcg cgcaagctta atcggttgcg ccaggaaggg ccggagattc
    14041 ccccggtggc gcgaccagcc gacgccggct cggaaaaacg cgccgcgtcg ttttcggtcg
    14101 gcccgcgcga cgacgcctga aacccgcgac agcgggcgcg gtgcctggga cgccttgggc
    14161 gagaatccga ttgtcttgac gcatgatgct gcgccggtaa tcgcgttcgg ccagaattga
    14221 tgccgcgtca gaagaacggg aagtggtctt cgtaactgag gtcgggcccg ggatggaagc
    14281 gcgtgacgtg cgaaccatcg cgcccgaagt acacgtacat gaacgaattc cagacctggt
    14341 cctgcttgta gcggtagctc cagacgattt gcgtgtccga cggcaggccg accgtgtcct
    14401 tgatggcggg tggaccgaat tcgcagcgca cctgctgggg tgtccaggtg cctttggcaa
    14461 gaatcttgaa atgcgcgtcg gtgagcaacg gcacgacgcg atctgtgtcg ccggcggcgt
    14521 cgacattggt gccccacgca tactggccag cgggctgctg ggtccagatc aggcgcttgc
    14581 cgccattggg cagggggcag gaaaagttcg gggcgccgaa tttcgcctcg atctgggcga
    14641 tgggcgtgcc gggcggcgta ttggccatgt tggcgcaggc gcccagggcc agggccgagg
    14701 ccgccgccag agcgcaacgg gcgatgcggc gattgatgcg gatcatgacg tgccttcctt
    14761 gttcggcgcc cggccacttt ctggccgacg cacccattct agccaatccg ttataatgcg
    14821 gccagatttc tgtttttttc aaccgtcacg tcagggcacc tcggctctgg cgcctgttca
    14881 tagaggcctc gaacaatgtc tgtagccgac atcaacaaat ccgatatcgt tgcgcaatac
    14941 cagcgcgccc agggcgatac cggctccccc gaagtgcaag tggcgctgct tacggcgcgc
    15001 atcaacgaac tcaccgacca tttcaagacg cacctgaaag accaccattc gcgtcgtggc
    15061 ctgctgcgca tggtcagccg tcgccgcaaa ctgctggact acctgaaggg ccgcaatccc
    15121 gattcgtacc gctcgctcat cgaaaagctc ggtctgcgta agtgatacgg ggggttggct
    15181 ccccatgatg caaccgtgcg caccgcgacc cgcttcgcga tgcgcacggt tttttaattt
    15241 aaggataccc cgcaatgttc aataaagtga ccaaatcgtt tcagtacggt cagcacacgg
    15301 tggttctcga aacaggcgaa atcgctcgcc aagcctcggg cgccgtcatg gtttcggtcg
    15361 acgacaccgt tattctggca tccgtggtgg cggccaagaa ggccaagccc ggccaggact
    15421 tcttccccct gacggtcgac tacgtcgaaa aaacctatgc cgcgggccgt atccccggtg
    15481 ggttcttcaa gcgcgaaggc aagccgtccg aaaaagaaac cctcacatca cgcctgatcg
    15541 atcggccgct gcgtccgttg ttccccgagg ggttctacaa cgacgtgcag gtcatcatcc
    15601 acaccatgtc ggtcaacccg gaagtcgacc ctgacattcc cgccatgatc ggcgcgtccg
    15661 ctgcgctggc catctctggc attccgttca acggccccat cggcgccgcg cgcgtggcct
    15721 acgtcaatga ccagtacgtg gtcaacccca ccaccacgca attgcacgac tccaagctga
    15781 atctggttgt tgccggcacc gaggccgccg tcctgatggt tgagtccgaa gccaggcaat
    15841 tgcccgaaga cgtcatgctg ggtgctgtcg tgttcggcca cgaacaaatg caggttgtca
    15901 tcaacgccat ccacgatctg gtggccgagg ccggcaagcc cgaatgggac tggcagcccg
    15961 cgcccaagaa cgaagcgttg gtcgcagccg tcaccgccgc cgcccaggaa ggcttgcacg
    16021 gcgcctacca gatccgtgaa aagcaggccc gcaccctgcg tctgcgcgaa gtctacgcca
    16081 gcgtcaagga caagctgctc gaaggcgcca ccgccagggg cgaagaggcc cccgacagcg
    16141 ttgaggtcga aaacatcctg ttcgacctgg aagccgcgct ggtgcgcagc cagatcctca
    16201 acggcgagcc gcgcatcgac ggccgcgaca cgcgtaccgt gcgccccatc agtgtgcgcc
    16261 tgggcgtgct gccgcgcgcc cacggcagcg ccttgttcac ccgtggtgaa acccaggccc
    16321 tggtggtcgc cacgctgggc accaagcagg acgagcagat catcgattcc atcatgggcg
    16381 agtaccgcga tcgcttcatg ttccactaca acatgccgcc gtacgcaacc ggcgaaaccg
    16441 gccgtttcgg cgtgcccaag cgccgtgaaa tcggtcatgg ccgcctggcc aagcgcgcat
    16501 tgctggcgct gctgcccgac catcaggatt tccagtacac catccgcgta gtgtccgaaa
    16561 tcacggaatc caatggttcc tcgtccatgg cgtcggtgtg cggtggctcg ctggccatga
    16621 tggacgccgg cgtgcccatc ggtgaccacg tggccggtgt ggccatgggg ctcatcaagg
    16681 aaggcggcaa gttcgccgtg ctgaccgaca ttctgggcga cgaagaccac ctgggcgaca
    16741 tggacttcaa ggtggccggc accgtgcaag gcatcaccgc cctgcaaatg gacatcaaga
    16801 tccagggcat caccaaggaa atcatgcagg ttgcgctggc gcaggcccgc gaaggccgct
    16861 tgcacatcct gggcaagatg aaggacgccc tcgaagggtc gcgcaccgaa ttgtccgaat
    16921 tcgcgccgcg catgctcagc atcaagatca atcccgagaa aatccgcgac gtcatcggca
    16981 agggcggcgc caccatccgc gcgcttaccg aggaaaccgg cacccagatc gacatcaacg
    17041 acgatggcac catcaccatc gccagcgccg acctcgatcg cgcccgtgaa gccgagcgcc
    17101 gcatcaagga actgaccgcc gacgtcgaag tgggccagga atacgaa
//
LOCUS       DASTNK010000095        21174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_252488, whole genome shotgun sequence.
ACCESSION   DASTNK010000095 DASTNK010000000
VERSION     DASTNK010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 21174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21174
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252488"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /locus_tag="VFR20_07320"
     CDS             <1..177
                     /locus_tag="VFR20_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEU0230364.1"
                     /translation="TYLRREQPFDTAGSAKAEGLGVALMERMTSDDPTAIIGLPLIEL
                     CRLLRNVGLDPLRT"
     gene            174..902
                     /locus_tag="VFR20_07325"
     CDS             174..902
                     /locus_tag="VFR20_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU0230365.1"
                     /translation="MTTPPSPGTLHLIPVGLGDAPTSSWLPGDAQTLVSRLTVYIAEN
                     AKTARAFLKLVGTTVPLQEITIHTLSEKTTDAQIEGWLTPLRNGGEIGLVSEAGCPAV
                     ADPGARVAAMAHRMALRVAPWVGPSSILLSLMASGLDGQRFAFHGYAAIEPAQRARQI
                     KTWEAESRRLRQTQLFIETPYRNGAMFDTLLQTLAGGTRLCVARDLTTASEWVRTAPI
                     AEWKTRPAPDLHRKPAIFLFLAAS"
     gene            complement(911..1564)
                     /locus_tag="VFR20_07330"
     CDS             complement(911..1564)
                     /locus_tag="VFR20_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU0230366.1"
                     /translation="MKRYSAVVFDWDGTVMDSTHAIVAAIQGACADLGLPIPDASRAS
                     WVIGLSLEAALYHAVPTLGADQMPLFLNSYRAHFMRRNTDMVLFEGVRELFDELAGRG
                     VSLAVATGKSRPGLDRVLAEGRLEHVFACTRCADEAPGKPDPTMLREIVDTLDLDPDC
                     VVMVGDTAHDVQMASNAGMDSVAVTYGAHDRQTLMLAKPTAMVSSVAELRDWLSDRT"
     gene            complement(1561..2511)
                     /locus_tag="VFR20_07335"
     CDS             complement(1561..2511)
                     /locus_tag="VFR20_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU0230367.1"
                     /translation="MHNPIDSSAPAVRFFQIDPEQAGQRVDNFLIRVCKGVPKSHLYK
                     AIRSGEVRVNKGRVGAEQRLEAGDLVRVPPLRLPRPDEPRKVPAAIFPVVFEDDALMV
                     IDKPAGVAVHGGSGVSFGVIEQLRAAYPDAPFLELAHRLDRETSGLLMIAKKRKALLG
                     LHDMMRQGLGRKHYLALVVGDWVNDRQHLRQPLARWLTDSGERRVRVDAREGKPAHTI
                     ITLEKRYGAYSLLGAELRTGRTHQIRVHLASCGFPIVGDEKYGRDDVRAAFSAQGFKR
                     MFLHACRLEIPHPITGATLDLRADPPPDCVQVLDRLERNA"
     gene            3034..5874
                     /locus_tag="VFR20_07340"
     CDS             3034..5874
                     /locus_tag="VFR20_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEU0230368.1"
                     /translation="MKRMLFNATHQEELRVAIVDGQKLIDLDIETAGREQRKGNIYKG
                     VITRIEPGLEACFVSYGEERHGFLPFKEIARSYFKEGVDVRTARIQDALSEGQELIIQ
                     VEKEERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRVEGEDRQELRETMDKLDLP
                     AGMSIIARTAGIGRNVEELQWDLSYLLQLWTAIDGAARENKAPILIYLESSLVIRAIR
                     DYFSPEIGEILIDTDDIAEQATAFMSVVMPDNVQRVKRYRDDIPLFSRFQIEHQIETA
                     YSRTVELPSGGSIVIDHTEALVAVDVNSARSTRGSDIEETAMRTNLEAAEEVARQLRL
                     RDLGGLIVIDFIDMEDSKNQRAVETRLRDALHFDRARVQMGKISRFGLMELSRQRLRP
                     ALNEGSHITCPRCNGTGVIRDAESSALQILRLLQEEAMKENTASLYAQVPVDVATFLL
                     NEKRGDITKIEARLKVNLVLIPNKALETPHHIIERLRHDDPRLEEIKPSFELASTPEE
                     EAPWQPNKGHETKARPEALVKGITPSQPAPVSSHATTAPVTTPSRPGLFKSLINWLTG
                     TSNASSTETAPSTPPARAPRSTSARAQSNQERREHGNGERRRRRNEPRQAIPEVDAAK
                     PAKAQTERPQRRNAESPSNGTDERRPVETDAADAAGPGRNGRNRRGRGRSRREDAPTV
                     ATTETNDTIRAVALDSTVTPAIVRDEEFEKPTSPAVSPSDTTPMIEAATSVEGEQADP
                     ERRRRRRRSRRGRRGGEAGDSLATASLPSMDGFVQTGEAEDSSVDDPQAGPDHHEASI
                     AAPSDAEPTPDIASVSAPQTSTEAAIPVATTEAEPEHVSQPELMATPEIPTTAIERAT
                     HHQEEQVATPVLATPPVEAQPEAAPAKQTKAGSASLEDVIAAAGLQWVETKAAAQPVP
                     DTIAAPRLGRARKPSTAVAPAPLEQVETHTHQ"
     gene            complement(6075..6974)
                     /gene="accD"
                     /locus_tag="VFR20_07345"
     CDS             complement(6075..6974)
                     /gene="accD"
                     /locus_tag="VFR20_07345"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEU0230369.1"
                     /translation="MSWIEKILPPRINRNNEAGAKRVPEGLWVKCPGCESVLYKEDLS
                     ATLNVCPKCSHHMRIGARKRIDSLLDPEGRVELGADIRPVDPLKFKDSRKYPERVAEA
                     VKLTGESDAMVVMAGSILSVSTVLACFEFAFMGGSMGSVVGERFARGVQHAIKHRMPF
                     ICVSASGGARMQESLFSLLQMAKTSAMLTRLAAEKLPFISILTDPTMGGVSASFAFMG
                     DVVIAEPKALIGFAGPRVIEQTVRETLPEGFQRAEFLLDKGAVDMVVDRRQLRSELAH
                     LLALLMRQPDDIVAKAGNSVGTP"
     gene            complement(7072..7911)
                     /gene="trpA"
                     /locus_tag="VFR20_07350"
     CDS             complement(7072..7911)
                     /gene="trpA"
                     /locus_tag="VFR20_07350"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU0230370.1"
                     /translation="MPISSSRLDSVFQRAAGRTALIPYVTAGDPSISATVPLLHALVA
                     AGADVLELGVPFSEPMADGPVIQRASQRAIDRGVGLRQVLSMVAEFRRGDANTPVVLM
                     GYANPIEHMGQAAFARAAAEAGVDGVLVVDYPPEEITDFTALLETAGIAPIFLLAPTS
                     TLQRIEQVARCARGYVYYVSLKGVTGAGHLDTSDVQRRVAELRNHLSVPIGVGFGIRD
                     ADSACRVAEFADAVVIGTRLIETIEAAVEAAGKPASTESDSAAIAAAAAWLGGIRAAL
                     DRA"
     gene            complement(7926..9125)
                     /gene="trpB"
                     /locus_tag="VFR20_07355"
     CDS             complement(7926..9125)
                     /gene="trpB"
                     /locus_tag="VFR20_07355"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009456612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU0230371.1"
                     /translation="MNTYSLPDENGHFGQYGGSFVAETLVHAIDELRDAYAKYRDDPD
                     FQTEFASELKHFVGRPTPVYHAKRWSERLGGAQIWFKREDLNHTGAHKINNCIGQILL
                     ARRMGKPRIIAETGAGQHGVASATVAARYGLECVVYMGSEDVRRQASNVYRMKLLGAT
                     VVPVESGSRTLKDALNEALRDWVTNVENTFYIIGTVAGPHPYPMMVRDFQSVIGNECL
                     TQMPDDAGRQPDVVVASVGGGSNAMGIFYPYIDHPDVELVGVEAAGDGLETGRHAASL
                     NAGSVGVLHGNRTYIMQDDNGQIIETHSISAGLDYPGVGPEHAWLKDSGRARYETVTD
                     TEALAAFHQCCRTEGIMPALESSHAIAYATRLAPTLPRDKIILVNLSGRGDKDMHTVA
                     DFSGIRL"
     gene            complement(9283..11532)
                     /locus_tag="VFR20_07360"
     CDS             complement(9283..11532)
                     /locus_tag="VFR20_07360"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU0230372.1"
                     /translation="MPTIIQPDAGVPVFGADWLDAVCADLDDAGRGLVARAVAYVREP
                     LVGGRASTGEPLDAHCAGVVRILADIGADAPTRASALIAVVPGSGEPASRGERDVVGL
                     DFGPEVLSLVRGTRALLRLGNRAGQSRDTTASSLGQKEMQRKMLLAMAADLRIVLMRL
                     ASRLQSLRWYAASRTECPQRFALETMELYTPLANRLGIWQLKWEMEDLAFRFLEPETY
                     RSIARLLEEKRVEREEFIEAVEARLRTALGDAHIPGDVSGRPKHIYSIWNKMRIKHLE
                     FDQLFDLRALRVIVNDERACYAVLALVHALWTPVPEEFDDYISRPKPNGYRSLHTVVT
                     DTDGKTFEVQIRTHAMHQFAEYGMAAHWRYKEAGARGGEIAAVGGYDRKIAWMRQLLA
                     WDGAGGETGTVARAPVAEASPAALRRPDEHIYVMTPQARVIELPVGSTPVDFAYYLHT
                     DLGHRCRGARVDGQMVPLLTRLSTGQTVEIIAAKSGGPSRDWLNPQLGFLASPRSRAK
                     VRAWFNAVELQNRISQGQVLVEKELQRLGKTAVNLEQLAQQLGFARADDLYVAAAKEE
                     FSLRQIDHALNQSQEAPSVPVESSAVVAPLAAASVARTGRSGVLVVGVDSLMTQLARC
                     CRPAPPDAIAGFVTRGRGVSIHRGDCASYQALLAHHPERVIDVSWGDTHDAFYPVDIS
                     IHAQDRPNLLRDLSDVFARLRIKVVGINTHSRRSLAHMVFTVEVHDGGEAGKALAALN
                     ELAGVTASR"
     gene            11608..12123
                     /locus_tag="VFR20_07365"
     CDS             11608..12123
                     /locus_tag="VFR20_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU0230373.1"
                     /translation="MNDRERKTLLIVWHSRTGTARQMKDAALRGALAILDELQAHEAL
                     EISVQAAEQAGTEDVLGADGYLFCAPENLATLSGAMKEFFDRTYYAALDRVNGRPYAL
                     MISAGTDGAGAVRLVERICAGWRLRAVAPALIARTGADTPETIAAPKTLAPGDQAACE
                     TLGGTLAALLV"
     gene            complement(12138..12491)
                     /locus_tag="VFR20_07370"
     CDS             complement(12138..12491)
                     /locus_tag="VFR20_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU0230374.1"
                     /translation="MSDIQRINPGKRMSDVVIHHGVAYLAGQVPDDATLGVEGQTAQV
                     LATIDKLLAEAGSDKSRILSAQVFLTSMSEFQGMNKAWDAWVAPGATPARATIEARLA
                     NPDFKVEIMVVAAVG"
     gene            complement(12717..13892)
                     /gene="pncB"
                     /locus_tag="VFR20_07375"
     CDS             complement(12717..13892)
                     /gene="pncB"
                     /locus_tag="VFR20_07375"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU0230375.1"
                     /translation="MIITSLLDTDLYKFSMMQVVLHHFPAAQVEYRYKCRTPGINLSP
                     YLDEIRAEIHALCELRFTDAELDYLSGLRFIKSDFVDFLGLFHLPGKCIHVSEGAVPG
                     EIDITVKGPWLHTILFEIPVLAIVNEVYFRNVCREPAWEEGRKRLQSKMRLVLDDPAL
                     ADFRIAEYGTRRRFSKQWHEEVLVTMKDRMGAHFAGTSNVRFAMMHGVTPLGTMAHEY
                     LQACQALGPRLRDSQVYALEVWAKEYRGDLGIALSDVYGMDAFLRDFDMYFCKLFDGA
                     RHDSGDPFQWGERLLAHYAANRTDPRTKTLVFSDGLTVPRAIELAKRFAGRCKVSFGI
                     GTNLTNDLGHEPLQIVMKMIRCNGQPVAKVTDAPEKTMCDDKAYLAYLRQVFQLPPA"
     gene            14077..14400
                     /gene="fdxA"
                     /locus_tag="VFR20_07380"
     CDS             14077..14400
                     /gene="fdxA"
                     /locus_tag="VFR20_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEU0230376.1"
                     /translation="MTHVVTENCIKCKFTDCVDVCPVDCFREGPNFLVIDPEECIDCA
                     VCVPECPANAIFAEEDVPQDQVGFIELNADLTPEFKTISRSIKALPEADDWNGVPDKL
                     QYLER"
     gene            14667..15893
                     /gene="phaZ"
                     /locus_tag="VFR20_07385"
     CDS             14667..15893
                     /gene="phaZ"
                     /locus_tag="VFR20_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEU0230377.1"
                     /translation="MLYDLHELQRSFLTPLAAFTDTGSQLFSHPCSPFSYTPVSRHIA
                     ATYELIHRLGKDYEKPAWDLDDTEIDGAAVPVICETAQATPFCKLLHFRRDTPAPRGQ
                     DPRVLLVAPLSGHHATLLRDTVRALLPAHDVYVTDWVDARMVPVAAGRFGLDDYVRTI
                     RDFIRKLGPDLNVISVCQPTVPVLAAISLMAAEGDACLPRSMVMMGGPIDARRSPTQV
                     NRLATTKPYEWFENNLIHRVPARYPGAGRPVYPGFLQHAGFIAMNPDRHLRSHYDFYL
                     HLLQNDDEDAETHRRFYDEYNAVLDMTAEFYLDTIRVVFQEYQLPSGTWIIDGQKVDP
                     SAITSVALLTIEGEIDDISGQGQTRAAHDLCSGIPSKRRQHYTAPGCGHYGIFSGRRW
                     RTTICPKIAAFIRNAA"
     gene            15952..16608
                     /gene="rsxB"
                     /locus_tag="VFR20_07390"
     CDS             15952..16608
                     /gene="rsxB"
                     /locus_tag="VFR20_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HEU0230378.1"
                     /translation="MNPALPTHATTSLADRIDALLPQTQCGKCGYDGCRPYAHAMAHD
                     GEAINRCPPGGDAGIHALAGLLGRPEIPLNTACGEHAAPTVAVIDEAHCIGCTLCIQA
                     CPVDAIMGANKRMHTVLADLCTGCDLCVAPCPVDCITMVPAGREWTPRLATVARLRHQ
                     RRQKRLAEQHPEASALASRRTLANKAPVTAAAHADDARKQRVIADAIARARARRAKPT
                     "
     gene            16675..17313
                     /gene="nth"
                     /locus_tag="VFR20_07395"
     CDS             16675..17313
                     /gene="nth"
                     /locus_tag="VFR20_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU0230379.1"
                     /translation="MNAEKRARIFSRLRAANPDPTTELEYASVFQLLIAVMLSAQATD
                     KSVNLATRRFFPDHGTPQALLALGEDALAEFIKTIGLYRTKAANIIKTCAILIERDNG
                     EVPDDRAALESLPGVGRKTANVVLNTAFGHPTIAVDTHIFRVANRTGLAPGKTVLDVE
                     RRLEKVVPAEFALNAHHWLILHGRYVCVARKPRCPQCGIADLCEYRHKTPPD"
     gene            17496..18269
                     /locus_tag="VFR20_07400"
     CDS             17496..18269
                     /locus_tag="VFR20_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0230380.1"
                     /translation="MSDLILETHHLTKEFRGFVAVDDVNFQVARGHIHALIGPNGAGK
                     TTCFNLLTKFLEPTSGRIIFSGHDITGEKPAQIARRGIIRSFQISAVFPQLTLLENVR
                     VGLQRATGQSFHFWRSDKCLNRLNDAAMALLAQVDLASYAQERTANLPYGRKRALEIA
                     TTLAMEPELMLLDEPTQGMGHEDVARVTELIKKVGVGRTILMVEHNMKVISSIADRIT
                     VLSRGSVLAEGDYQEVSRNPEVMQAYMGTEVGELEGAQA"
     gene            18266..18979
                     /locus_tag="VFR20_07405"
     CDS             18266..18979
                     /locus_tag="VFR20_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0230381.1"
                     /translation="MSAVLEITDLHAWYGESHVLHGVNMQVNQGEVVTLLGRNGSGRT
                     TTLRATLGLTGSRKGSIRIGGTESIALPTYKIAHLGIGYCPEERGIFAGLSCEENLLL
                     PPPVGASTLGGGMSLAEIYDMFPNLQERRYSPGTRLSGGEQQMLAVARILRTGANLLL
                     LDEISEGLAPVIVQALARMITTLKAKGYTIVMVEQNFRFAAPLADRFYVIEHGQVVEH
                     FNAAELPEKQDVLNTLLGV"
     gene            19032..20249
                     /locus_tag="VFR20_07410"
     CDS             19032..20249
                     /locus_tag="VFR20_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0230382.1"
                     /translation="MKLRILSAALATAGLGLAMAGPSHAAGISDNVVRIGFITDMSGV
                     YSDIDGKGGVEAIKMAIADAGGKIDGKKIELFTADHQNKADIGSARARQWFDEKHIDM
                     LVGGTNSAVNLAMAAVAAEKQKPFFAVGPGSSNLTNSACTHYTIHYGYDTVALARGTA
                     NYMAEHGDKTWYFLTADYAFGHSLQRDATKVIDAAGGKVVGSVNVPLGTSDFSSFLLQ
                     AQSSKAQVLALANAGGDFVNSMKAAQEFGITKTMKIAGLLVFINDIHSLGLKATQGLY
                     LTNAWYWDKDAASRKFAERFYKKEHRMPSFLQAGDYSAVSFYLRGVKATGTDNGNDMM
                     KWIKSHKVNDFFADDGYVREDGTLIHNMYLMQVKTPAESKKPWDYYKLVKVLPGDKIY
                     TSLAQSTCKFVKK"
     gene            20344..>21174
                     /locus_tag="VFR20_07415"
     CDS             20344..>21174
                     /locus_tag="VFR20_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0230383.1"
                     /translation="MTELFGIPVQALLGQLLLGLVNGAFYAVLSLGLAVIFGLLNVIN
                     FAHGALYMLGAFLAWMGLQYLGLNYWVMLILAPILVGIFGIIIERLLLKHLYKLDHLY
                     GLLLTFGLTLLIEGLFRSLYGVSGQPYSTPDLLQGGVNLGFMYLPIYRAWVIVASLVV
                     CFATWAVIEKTRLGALLRAGTENPKLVEAFGVNVPLVITLTFGFGVGLAGFAGVLAAP
                     ILQVSPLMGSNLIIVVFAVVVIGGMGSILGSIVTGLGLGVIEGLTKVFWPEASTTVVF
                     I"
BASE COUNT         4176 a         7159 c         6231 g         3608 t
ORIGIN      
        1 acgtatctgc ggcgcgaaca gcccttcgac accgccggta gcgcgaaagc cgaaggcttg
       61 ggtgtcgcgc tgatggaacg catgacctcg gacgacccca cggccatcat cggcctgcct
      121 ctcatcgaac tctgccgcct gttgcgcaat gtcggcctgg atccactacg cacatgacca
      181 cccctccctc ccccggcacc ctgcacctca ttccagtcgg cctgggcgat gcgccgacct
      241 cgtcctggct gcccggcgat gcacagacac tggtatccag gcttaccgtc tacatcgccg
      301 aaaacgccaa gactgcccgg gcctttctca agctcgtcgg gacgaccgtg ccactgcagg
      361 aaatcaccat ccacaccctg agcgaaaaaa ccactgatgc gcagatagaa ggctggctga
      421 cgccactacg caacggcggt gaaatcgggc tggtttcgga ggcgggctgt cccgccgtgg
      481 ccgatcccgg ggcccgcgtg gcggctatgg cgcaccgcat ggcgctgcgc gtggcccctt
      541 gggtaggccc ctcatccatt ctgctgtccc tgatggcgag cggcctggat ggccagcgct
      601 tcgccttcca tggctacgcc gccatagagc cagcccagcg ggcacgccag atcaaaacct
      661 gggaagccga atcgcgccgg ctgcgccaga cgcagctgtt catcgaaacc ccttatcgca
      721 atggggcgat gttcgacacc ttgctgcaga cactggctgg tggaacacgc ctgtgcgtgg
      781 cgcgcgactt gacgaccgca agcgaatggg tgcgcacggc cccgatcgcc gagtggaaaa
      841 cccggccagc ccccgacctg cacagaaagc ccgccatctt cctgtttctg gcggcttcct
      901 gatcataaag tcaggtgcga tcactcaacc aatcgcgcag ctccgcgaca cttgaaacca
      961 tggcggtcgg cttggccagc atgagggtct gccggtcgtg cgcgccatag gtgaccgcca
     1021 cgctatccat ccccgcgttc gacgccatct gcacgtcgtg cgccgtatct cccaccatga
     1081 caacgcaatc cggatcgaga tccagcgtgt cgacgatttc ccgcagcatg gtcggatcag
     1141 gcttgccggg cgcctcgtcc gcgcaacgcg tacaggcgaa cacgtgttcc aggcgaccct
     1201 ccgccaggac gcggtccagg ccaggccggg acttgcctgt ggcgaccgcc agcgacacgc
     1261 cgcgcccagc cagttcgtcg aagagttcac gcacgccttc gaacaggacc atgtcggtgt
     1321 tccggcgcat gaaatgcgcg cgatagctat tcaaaaacag cggcatctgg tccgccccca
     1381 gtgtgggcac ggcatgatag agcgccgctt cgagcgaaag ccctatcacc cagctcgcgc
     1441 gcgacgcgtc gggtatgggc agcccgagat cggcgcaggc cccctggatg gccgcgacga
     1501 tggcgtgcgt ggaatccatg acggtcccat cccaatcgaa gacgacggcg ctgtatcgct
     1561 tcatgcgttc cgctccaggc gatccagcac ctggacgcaa tcggggggcg gatcggccct
     1621 cagatcgagc gtcgcgccag tgatcggatg cggaatctcg aggcggcacg catgcaggaa
     1681 catgcgcttg aacccttgcg ccgaaaatgc cgcgcgcaca tcgtcccgcc cgtatttttc
     1741 gtctcccacg atggggaagc cgcaagaagc gaggtgcacc cgaatctgat gggtgcgccc
     1801 cgtgcgcaat tcagccccta gcaggctgta ggcgccatag cgcttctcga gcgtgatgat
     1861 ggtatgggcc ggcttgcctt cccgcgcatc gacccgcacg cggcgctcac ccgaatcagt
     1921 cagccagcgc gcaagcggtt ggcgcagatg ctgacggtcg ttgacccagt cccccaccac
     1981 cagggcgaga tagtgtttgc gcccaagacc ctggcgcatc atgtcgtgca ggcccagcag
     2041 cgccttgcgt ttcttcgcga tcatcaggag cccggacgtt tcacggtcca gacgatgcgc
     2101 cagttccagg aacggcgcat cggggtacgc ggcgcgcaac tgttcgatga cgccaaacga
     2161 caccccgctg ccaccatgca ccgccacacc ggccggtttg tcgatcacca tcaaggcgtc
     2221 gtcctcgaaa accacaggga agatcgccgc cggcactttg cgcggctcgt ccggccgagg
     2281 cagcctcaag gggggcaccc ggaccagatc gccagcctcg aggcgttgtt cggcacccac
     2341 ccggcccttg ttgacacgca cctcaccgct gcgtatggcc ttgtacagat ggcttttcgg
     2401 cacccccttg cagacccgta tgaggaaatt atcgacacgc tgccccgcct gctcgggatc
     2461 gatttgaaaa aaacggacgg cgggcgcgga ggaatcgatg ggattgtgca tgaaaaaagc
     2521 ggctataatc gcttcagcct atagaagctg agaaaacgcc cccggcgtag agatgcaatt
     2581 ggatgcgtct ccgtcgagtc ggaaaggcca agtcgcattg tactgcctgc gtttcagctt
     2641 ccgggtcaca aggtggtcgc cgggtctgtt ccggcctgcg tggcggcggc atggcatccg
     2701 tgccggcccc cccccaggcc gatacccttc gtttgcggca cagaagttct tgcaacacag
     2761 gtgatttcgt atcgttttgc accagccgcg cgaccccgcg cgactgccgt tcccagctga
     2821 tttcgtcaac cactgcattt atcgtgaacc tgacttccct gctgactgaa cctcgcgccc
     2881 ggaggcgcga cgtgcccttc tgataccggg acgggcacgg ccggtatcgc cagcccgact
     2941 ccccctgtgg cgggaaccag tcccgcagca caccgagtga tccgcggtcg cgcgccgaac
     3001 aacggcgcgc catgactacg gagaaagcca ctcatgaaac gcatgttgtt caacgcgacg
     3061 caccaagaag aattacgcgt cgccatcgtc gatggacaga aactcatcga cctcgacatc
     3121 gaaacagccg gacgcgaaca acgcaaggga aacatctaca aaggtgtcat cactcgcatt
     3181 gaaccgggtc tggaggcctg tttcgtcagc tacggcgaag agcgccacgg tttcctgccc
     3241 ttcaaggaaa tcgcccgcag ctacttcaag gaaggcgtcg acgtccgcac cgcccgcatt
     3301 caggatgccc tcagcgaagg ccaggaactc atcatccagg tcgaaaaaga agagcgcggc
     3361 aacaaagggg cggccctcac gactttcatt tcgctggctg gccgctatct ggtcctgatg
     3421 ccgaacaacc cgcgcggggg cggtgtgtcg cgccgcgtgg agggcgagga ccgccaggaa
     3481 ctgcgcgaaa ccatggacaa gctcgacctg cccgcgggca tgagcatcat cgcgcgcacc
     3541 gccgggatcg gccgcaatgt cgaagaattg cagtgggatc tgtcctatct gctgcaactc
     3601 tggaccgcca tcgatggcgc cgcgcgtgaa aacaaggcgc ccatcctcat ctacctcgag
     3661 tccagcctgg tcatccgcgc cattcgcgac tacttctcgc cggagatcgg cgagatcctc
     3721 atcgataccg atgacatcgc cgagcaggcc accgccttca tgagcgtggt catgccggac
     3781 aacgtccagc gggtgaaacg ctaccgcgac gatatccctc tgttctcgcg cttccagatc
     3841 gaacaccaga tcgaaaccgc ttattcacgc accgtggagc ttccatcggg cgggtccatc
     3901 gtcatcgatc acacggaagc cctggtggcc gtcgacgtca actcggcgcg ctccacgcga
     3961 ggatccgaca tcgaagaaac cgccatgcgc accaacctgg aggccgccga agaggtcgcg
     4021 cgccagctgc gcctgcgtga cctgggcggc ctcatcgtca tcgacttcat cgacatggag
     4081 gacagcaaga atcaacgggc cgtggaaaca cgtctgcggg acgcgctgca cttcgatcgc
     4141 gcacgcgtgc agatgggcaa gatttcccgc ttcggtctca tggaattgtc acggcagcgc
     4201 ctgcgccccg cgctcaacga gggctcgcac atcacctgcc cgcgctgcaa cggcacaggc
     4261 gtcatccgcg acgccgaatc cagcgcgctg caaattctgc gtctgctgca ggaagaggcc
     4321 atgaaggaga acaccgcctc cttgtacgcc caggtacctg tggacgtcgc aaccttcctg
     4381 ctcaacgaaa aacgcggcga catcaccaag atcgaggccc gtctcaaggt caacctggtc
     4441 ctgatcccca acaaggcgct cgaaacaccg caccacatca tcgagcgcct gcgccacgac
     4501 gacccaaggc tcgaagagat caagcccagc ttcgaactgg ccagcacgcc cgaggaagag
     4561 gctccgtggc aacccaacaa ggggcacgag accaaagccc ggcccgaagc cctggtcaag
     4621 ggcatcaccc cgtcccaacc cgcgcctgtg tcgtctcatg cgacgacggc gcccgtcacc
     4681 accccgtccc ggccggggct gttcaagagc ctcatcaatt ggctgacagg caccagtaac
     4741 gcctccagca ccgagaccgc tcccagcacg ccgccggcac gcgcgccccg ctcgacttcc
     4801 gcccgggcac aatccaacca agagcgacgc gagcatggaa atggcgaacg gcgtcgccgg
     4861 cgcaacgagc cgcgccaagc cattcccgag gtcgatgcgg cgaagccggc caaggcgcaa
     4921 accgagcgtc cccagcgccg caacgccgag tcaccgagca atggcaccga cgagcgccgc
     4981 ccggttgaaa cagatgccgc ggacgccgcg ggcccgggcc gcaacggtcg aaaccgtcgt
     5041 ggacgtggcc gcagccgccg cgaagacgca ccaaccgttg ccaccacgga aaccaacgac
     5101 acgatacgag ccgtcgccct cgacagcacc gtgacaccgg ccatcgtgcg cgacgaagag
     5161 ttcgaaaagc caacctcacc agccgtttcc ccgtccgaca ccacgccgat gatcgaggcc
     5221 gcgacgtccg ttgagggcga acaagccgat ccggaacgtc gccgccggcg gcgccgcagc
     5281 cgtcgcggtc gccgtggcgg tgaagcgggc gacagcctgg ccacggcatc gctgccgagc
     5341 atggatggct tcgtccagac gggcgaggcg gaagattcca gcgttgacga cccacaggcg
     5401 ggaccggacc accacgaggc gtcgatcgcc gcgccttccg acgccgaacc gacgcctgat
     5461 atcgccagcg tctcggcgcc acaaacctcc accgaagccg cgattcccgt ggcaacgacc
     5521 gaggccgaac cggaacacgt cagtcagccg gaactgatgg ccactcctga aattccgacg
     5581 acggcgatcg aacgcgcgac acaccaccag gaggagcagg tagccacacc ggtgctggcc
     5641 acgccgcccg tcgaagcgca acccgaagcc gcacccgcca aacaaaccaa ggcgggttcg
     5701 gcatccctgg aagacgtgat cgccgccgcg ggcctccaat gggttgaaac caaggcggcc
     5761 gcgcaacccg tgccagacac cattgccgct ccgcgcctgg gtcgcgcacg caaaccgtcc
     5821 accgccgtcg ccccggctcc tctggagcag gttgaaacgc acacgcatca gtaggcgcgg
     5881 tcggcaatcc cgccaggtcc acggcttcaa tacctggcgg gatacggcgc accggtgcca
     5941 tggaccatac ctgaaagccg tcagtctctg acagaacaaa aaaagcgccc atttcgatgg
     6001 gcgctttttt tgttccggcc tgcgtttgct gcggtgccac caccgcaggc gaaaatgcgc
     6061 gatacgagtg cgtgctaagg ggtgccgacc gaattaccgg ccttggcgac gatatcgtcg
     6121 ggctggcgca tcaacaacgc aagcaagtgg gccagttcac tgcgaagctg gcggcggtcg
     6181 accaccatgt ccacggcccc tttgtcgagc aggaactcgg cgcgttggaa gccctcgggc
     6241 agggtctcgc gcacggtttg ttcaatcacg cgcggcccgg cgaagccgat caacgccttg
     6301 ggctcggcga tgaccacgtc gcccatgaaa gcaaaactcg ccgacacacc acccatggtg
     6361 gggtcggtca gaatgctgat gaaaggcagc ttctcggcgg ccaggcgggt gagcatggcg
     6421 ctggtcttgg ccatctgcag caacgaaaac aggctttcct gcatgcgcgc cccgccggag
     6481 gccgacacac agatgaacgg catgcgatgc ttgatggcgt gctgcacgcc gcgcgcgaac
     6541 cgttcaccga cgaccgaccc catggacccc cccatgaacg cgaattcgaa acacgccaga
     6601 acggtggaga cggaaagaat gctgcccgcc atcacaacca tggcgtcgga ttcaccggtc
     6661 agcttcaccg cctcggccac acgctccggg tatttgcgtg aatccttgaa tttcaagggg
     6721 tcgacagggc ggatgtcagc gccaagctcc acacgccctt cggggtcgag cagcgagtcg
     6781 atgcgctttc ttgcaccgat gcgcatgtga tgagagcatt tcggacaaac attgagtgtc
     6841 gccgacagat cttccttgta cagcaccgac tcgcaccccg ggcacttgac ccataaccct
     6901 tcgggaacgc gcttcgcgcc ggcctcgttg ttgcgattga ttcgcggtgg aagaatcttt
     6961 tctatccaac tcattctgtg gtatccccga taaatggatg gcgccgatcc ggcgcggtgc
     7021 ctggccggcc acaaccggac gcggtactga gcgccacagt cgcggtccgc atcaggcgcg
     7081 gtccagtgcg gcgcgaatgc cgcccagcca cgccgcagcc gcggcgatgg cggcgctgtc
     7141 gctttcggtc gatgccggct tcccggcggc ctcgaccgcg gcttcaatgg tttcgataag
     7201 acgggtgccg atgacgaccg catcggcgaa ctcggccacg cggcatgcgc tgtcggcatc
     7261 acggatgccg aaccccacgc ctatgggcac ggacaggtga ttgcgcaatt ccgcgacacg
     7321 ccgctgaaca tcggacgtgt cgagatggcc ggccccggtc acccctttca atgagacgta
     7381 atagacgtat ccgcgggcgc aacgggccac ttgctcgatg cgttgaaggg tggatgtcgg
     7441 cgccagcagg aatatgggcg cgatgccagc ggtctcgaga agcgcggtga aatcggtgat
     7501 ttcctcgggc ggataatcga ccacgagcac cccatcgacg cccgcctcgg cggcggcgcg
     7561 ggcgaacgcg gcctgcccca tgtgctcgat ggggttggca taccccatca gcacgacggg
     7621 cgtgttcgcg tcgccgcggc ggaattccgc caccatggac aggacctgcc gcaggcccac
     7681 gccacggtcg atggcacgct gggacgcgcg ctggatcacc gggccatcgg ccatgggctc
     7741 ggaaaacggc acgccaagct ccagcacgtc ggcgccagcc gcaaccagcg catgcagcaa
     7801 cggcacggtg gcggagatcg acggatcacc cgcggtaacg tagggaatca gggcggtgcg
     7861 gccagcggcg cgctggaaga cggaatcgag acgggaggag gaaattggca tgaccttgga
     7921 tgtcgttaca agcgaatgcc gctgaaatcg gccacggtgt gcatgtcttt gtcgccgcgt
     7981 ccggacagat tgaccaggat gatcttgtcc ctgggcaggg tgggcgccag tctcgtggca
     8041 taggcaatgg catgcgacga ttccagcgcg ggcatgatac cttcagttcg gcaacattgg
     8101 tggaaggcgg ccagcgcctc ggtgtcggtg acggtttcat agcgcgcacg cccgctatcc
     8161 ttcagccagg cgtgctcggg gccgacgccg ggataatcaa gcccggccga aatggaatgc
     8221 gtttcgatga tctgcccgtt gtcgtcctgc atgatgtaag tgcgattgcc atgcagcaca
     8281 ccaaccgagc cagcgttcag ggatgccgcg tgccgtccgg tttccagacc gtctccggcg
     8341 gcctcgaccc ccaccagttc cacgtccggg tggtcgatgt atgggtagaa aatccccatg
     8401 gcgttggatc ctccgcccac cgaggcgacg accacatcgg gctgccgccc ggcgtcgtcg
     8461 ggcatctggg tcaggcactc attgccgatg accgattgga agtcgcgcac catcatggga
     8521 tacggatgcg ggccagcgac ggtgccgatg atataaaacg tgttctcgac attggtgacc
     8581 cagtcgcgca gggcttcatt cagagcatcc ttgagcgtgc gggagcccga ctccacgggc
     8641 accaccgtgg cgccaagcag tttcatgcga taaacattgg atgcctgccg gcgcacgtct
     8701 tcgctgccca tgtacaccac gcactccagc ccgtagcgcg cggcgaccgt ggcgcttgcc
     8761 accccatgct ggccggcgcc ggtctcggcg atgatgcgcg gtttgcccat gcgccgcgcc
     8821 aacaggatct ggccgatgca gttgttgatc ttgtgcgcgc ccgtatggtt gaggtcctcg
     8881 cgcttgaacc agatctgcgc gccgcccagg cgctcggacc agcgcttggc gtggtacacc
     8941 ggcgtgggcc gccccacgaa gtgtttgagt tcgctggcga attccgtttg gaaatcgggg
     9001 tcgtcgcggt acttggcgta cgcgtcgcgc agttcgtcga tggcgtgcac cagggtttca
     9061 gccacgaacg aaccaccgta ttgcccgaaa tgcccgttct cgtcgggcaa ggagtatgta
     9121 ttcaagggag gcactctctg gttacgctca agcaccgcgc cgccgggggc ccccgacgcc
     9181 gggcgtaatc gggtatttta ccagcccgtg gcttcatcgc acgaccggcg cgggcacggc
     9241 ccgcccccga cgccaccagg gcttgttccg tctcaaggaa cctcaacgac tcgccgtcac
     9301 acccgccaac tcgttgagcg cggccagcgc cttgccagcc tcgccgccat catgcacctc
     9361 gacggtgaac accatgtgcg ccagcgaacg ccggctgtgt gtgttgatgc ccacgacctt
     9421 gatgcgcagg cgcgcgaaca catctgacag atcgcgcagc aggttcggac gatcctgggc
     9481 gtggatgctg atatccaccg gatagaacgc atcatgcgta tcaccccagc tcacatcgat
     9541 cacgcgctca gggtggtgcg ccagaagcgc ctgataagac gcgcagtcac cgcgatggat
     9601 ggagacgccc cggccgcgcg tgacgaaacc cgcgatcgca tccggcggtg ccggccgaca
     9661 gcaccgcgcc aactgcgtca tcagggaatc caccccgacg acgagcacac cgctcctgcc
     9721 tgtcctggcg acactggccg ccgccaaggg cgccacgacg gccgatgact cgacggggac
     9781 ggatggcgcc tcttgcgact gattcagcgc gtggtcgatc tggcgcaggc tgaactcttc
     9841 cttggccgcg gcgacgtaca gatcgtcggc gcgggcgaaa cccagttgct gtgcgagctg
     9901 ctcgaggttg acggcggtct tgcccaggcg ttgcaactct ttctcgacca gcacttgtcc
     9961 ctgcgagatg cggttctgca attcgaccgc gttgaaccac gcccgcacct tggcgcgtga
    10021 gcgcgggctt gccagaaacc cgagctgtgg attgagccaa tcgcgtgatg gccccccgga
    10081 cttcgccgca atgatctcca cggtctggcc ggtggacagg cgggtcagca acggcaccat
    10141 ctggccatcg acccgtgcgc cccggcagcg gtgccccagg tcggtgtgca ggtaataggc
    10201 gaaatccaca ggggtcgagc ccacgggcaa ttcgatgact cgagcctggg gcgtcatgac
    10261 atagatatgc tcgtcgggac ggcgcagcgc ggcgggcgac gcctccgcca cgggcgccct
    10321 ggcgaccgtc cccgtttcgc cgccagcgcc atcccatgcc agcaactgac gcatccaggc
    10381 gatcttgcgg tcgtacccgc ccaccgccgc gatttcgccc ccccgcgcac ccgcctcttt
    10441 gtagcgccag tgcgcggcca tcccgtactc ggcgaattgg tgcatggcgt gtgtgcgtat
    10501 ctgtacctcg aaggtcttgc catccgtgtc ggtcaccacg gtatgcaatg aacgataacc
    10561 gttgggtttg ggccgcgaga tgtaatcgtc gaactcctcc ggaaccggcg tccacagcgc
    10621 atgcaccaac gccagcaccg catagcacgc acgctcatcg ttcacaatca cacgcagcgc
    10681 acgcagatcg aacaattgat cgaattccag atgcttgatg cgcatcttgt tccagatgct
    10741 gtagatatgc ttgggccggc ccgatacgtc cccggggatg tgggcatcgc ccagcgcggt
    10801 gcgcaggcgc gcctccaccg cttcgatgaa ttcctcacgt tccacgcgct tttcttcgag
    10861 caagcgggcg atggatcggt aggtttcagg ctcgaggaaa cgaaaagcca ggtcttccat
    10921 ttcccacttg agctgccaga tgcccagtcg attggccaag ggcgtgtaga gctccatggt
    10981 ttcaagcgca aaccgctggg gacactcggt gcgcgacgcg gcataccagc gcagcgactg
    11041 aagacgcgac gccagacgca taaggacgat gcgcaggtcg gccgccatgg ccagcagcat
    11101 cttgcgctgc atttcctttt ggccgagact ggaggcagta gtgtcacggg actggccagc
    11161 ccggttgccc agccgcaaca aggcgcgtgt gccgcgcacc agcgacaaca cctcggggcc
    11221 gaaatccaga cccaccacat cccgttcgcc gcgcgacgcg ggctcgcccg atccaggcac
    11281 gacggcaatg agcgcactgg cacgcgtggg cgcgtcggcg ccgatgtccg ccagtatccg
    11341 caccacaccg gcacaatgcg catccagcgg ttcacccgtg ctggcccgcc cgccaacgag
    11401 cggctcccga acgtacgcga ccgcgcgcgc caccagaccc cttccggcgt catccaggtc
    11461 ggcacagact gcgtccagcc agtcggcacc aaataccggc acaccggcgt caggttggat
    11521 tatcgtaggc ataacgcagc acacaacggg gatttgtatg aacgggcacg cctcgcgccc
    11581 cgcacgggaa aggataccgc attccagatg aatgaccgcg aacgcaaaac cctgctcatc
    11641 gtctggcatt cccgtaccgg aacggcccgc cagatgaaag atgccgcctt gcgcggcgcc
    11701 ctggccatcc tcgacgaact gcaagcgcac gaggcgctgg aaatcagcgt ccaggccgca
    11761 gaacaggctg gcacagaaga tgtgctcggc gccgacggct atctgttctg cgcgcccgag
    11821 aatctggcga cgttgagcgg cgccatgaag gaattcttcg accgcaccta ttatgccgcg
    11881 ctggaccgtg tcaacggccg cccctatgcg ctgatgatca gcgccggcac ggatggagcc
    11941 ggggcggtgc ggctagtgga gcgcatctgc gcgggttggc gcctgcgcgc cgtggcgccg
    12001 gcgctgatcg cccgcaccgg tgccgacacg cctgagacaa tcgccgcgcc caagacgctc
    12061 gcgccgggcg accaggccgc gtgtgaaact ttgggcggca cgctggccgc cctgctggtc
    12121 tgacgcgcta tcgagtgtca gccgaccgcg gcgaccacca tgatttccac cttgaagtcc
    12181 gggttcgcca gacgcgcctc gatggtggcg cgcgcgggcg tggcgccagg cgccacccaa
    12241 gcatcccatg ccttgttcat accctggaat tcactcatgc tggtcagaaa cacctgggcc
    12301 gacaggatgc gcgatttgtc ggacccagct tcggccagga gcttgtcaat cgtcgccagc
    12361 acctgggccg tctggccttc caccccaagc gtggcgtcat cgggcacttg ccccgcaaga
    12421 tacgccacac catggtggat gaccacgtcc gacatgcgtt tgccgggatt gattcgctgg
    12481 atgtcgctca tagctattgc tccaaaaacc gaaaagacgt cgtgaggcag acgccgaaga
    12541 ccccccgcca ctgcgcgcgt cccgcatgcg cggaagatgc cagatgcgcg ggcaaggaac
    12601 aagaacccgg cattctagcc catggctggc gcggacagcc ggcgtgccgt ctgatcaact
    12661 cgcggaccga ccatccggac tcgcaagcgc gcatgggcca aacggcgcac ctgaactcag
    12721 gctggcggca actgaaacac ttggcgaaga tacgccagat acgccttatc gtcgcacatg
    12781 gttttctcgg gcgcgtcagt gaccttggcc accggctggc cattgcaacg gatcatcttc
    12841 atcacgatct gcaacggctc gtggcccaga tcgttggtca ggttggtgcc gatgccgaac
    12901 gacactttgc agcgccccgc gaaccgcttg gccaactcaa tggcgcgcgg cacggtcagg
    12961 ccgtcggaga acaccagggt cttggtgcgt gggtcggtgc ggttcgccgc ataatgcgcc
    13021 agcagacgct cgccccactg gaatggatcg ccggagtcgt gcctagcgcc atcgaagagc
    13081 ttgcagaaat acatatcgaa gtcgcgcagg aacgcatcca tgccatatac gtcggacagc
    13141 gcgatgccca ggtcgccacg atattccttg gcccagactt ccagcgcata aacctgcgaa
    13201 tcgcgcaggc gcggacccag cgcctgacac gcctgcaggt actcgtgagc catcgttccc
    13261 agtggggtga cgccatgcat catcgcgaac cgcacgttgc tggtgccggc gaaatgcgct
    13321 cccatccggt ctttcatcgt gaccaggact tcctcgtgcc actgtttcga gaaacgccgc
    13381 cgcgtgccgt actccgcgat gcggaaatcg gccagagccg ggtcatcgag caccagccgc
    13441 atcttcgact ggagccgctt gcgcccttcc tcccatgcag gttcccggca cacgttgcgg
    13501 aagtacacct cgttgacgat ggccagcacc ggtatttcga acagaatggt atgcagccac
    13561 ggacccttga ccgtgatgtc gatctcgccg ggcaccgcgc cttcggacac gtggatgcat
    13621 ttcccgggaa gatggaacag gccaagaaaa tcgacgaaat cgctcttgat gaaacgcaac
    13681 ccgctcaggt agtccagttc ggcgtcggtg aaccgaagct cgcacaacgc atggatctcg
    13741 gcgcggatct cgtcgagata cgggctgagg ttgatgcccg gtgtacggca cttgtagcga
    13801 tactcgacct gggctgcggg aaagtgatgc aacaccacct gcatcatgct gaacttgtag
    13861 aggtcggtgt caagaagaga ggtgatgatc atcgtgcgcc agcgtggtct ttcccaaacc
    13921 atagcacagc gcgtggcgat tttccgatca tgcgtggcgc accgcaaaga ctgacagcac
    13981 catcgggttt atccggagag ccacgcgata attcgttaaa atcgcagagt ctggcaatcg
    14041 atattcgttg ccctgtttca agccggagcc gcatcaatga cccacgtcgt gaccgaaaac
    14101 tgtatcaagt gcaaattcac ggattgcgtc gacgtgtgcc cggtggactg ctttcgcgag
    14161 ggcccgaatt tcctggtcat cgacccggaa gagtgcattg actgcgcggt gtgcgtgccg
    14221 gaatgcccgg ccaacgccat cttcgccgaa gaagacgtac cccaggacca ggtcggattc
    14281 atagaattga acgccgacct cacacccgaa ttcaaaacca tcagccgctc gatcaaagcc
    14341 ttgcccgagg ccgacgactg gaacggtgtt cccgacaaat tgcagtatct ggaacgctga
    14401 acggccggca acactcccgc ccgcacccct gcggcgtcgg gtttgttgca cagcagcaac
    14461 aaatttacgg tttttgtgta tacatcaccc tgcggattag ggaattccgg gagggtttac
    14521 atctattcac cctttgcttt ttgtgattag ctaataaaaa gaaaccgttt tcttttcggg
    14581 ttttcgtcat aggccgacca caagtcaaac cctgcggccc caacctgagt gcgagaagcc
    14641 ccattcacaa gcaaaggcat tcgtccatgc tgtacgacct acacgaactg cagcgttcgt
    14701 tcctcacccc gctggcggct ttcaccgaca cgggctcgca gttgttctcc cacccatgca
    14761 gcccgttttc gtacacaccg gtgtcgcgcc atattgccgc cacctacgaa ctcatacatc
    14821 gactgggcaa agactatgaa aagccagcct gggatctgga cgacaccgag atcgatggcg
    14881 cagccgtccc ggtcatctgc gaaaccgccc aggccacccc attctgcaag cttctgcatt
    14941 ttcgccgcga cacgccggcc ccccgagggc aggatccccg ggtgctgctg gtcgcgccgc
    15001 tgtccggaca ccatgccacg ctgttgcgcg acacggtgcg ggcactgctg cccgcgcacg
    15061 acgtctatgt cactgactgg gtcgacgccc gtatggttcc cgtggcagcc ggccggttcg
    15121 ggctggacga ctacgtccgc acgatccgcg acttcatccg caagctgggc ccggatctga
    15181 acgtgatttc cgtctgccag cccacggtgc cggtgctggc ggccatatcg ctcatggccg
    15241 cggagggcga tgcctgtctg ccccgcagca tggtcatgat gggcggcccc atcgacgcgc
    15301 gccgctcgcc cacccaggtc aaccggctgg cgacgaccaa accgtatgaa tggttcgaga
    15361 acaatctcat ccatcgcgtg ccggcgcgct atcccggcgc gggccgtccg gtctatccgg
    15421 gctttctgca gcacgccggc ttcatcgcca tgaaccccga tcggcatctg cgctcgcact
    15481 acgattttta cctgcatctg ctgcagaacg acgacgagga cgcggaaacg catcggcgtt
    15541 tctacgacga atacaatgcg gtgctcgaca tgacggcgga gttctatttg gacaccatcc
    15601 gggtcgtgtt ccaggaatac cagctgccct ccggcacctg gatcatcgat ggccaaaaag
    15661 tcgatccatc cgccatcacc agtgtcgccc tgctgaccat cgaaggcgag atcgacgata
    15721 tctcggggca gggccagacg cgcgcggcgc acgatctgtg ctccggcata ccgtcaaagc
    15781 ggcgccagca ctatacggct ccggggtgcg gccactatgg catattctcg ggccgccgct
    15841 ggcgcacgac gatctgtcct aaaatagcgg cgttcatccg caacgcggcc tgaccccggc
    15901 aaccgcgtcc gtgtggcccg gcaccttcca cgggccgggc ctttcgtctt catgaatcct
    15961 gcgctcccca cccacgccac cacgtctctc gccgaccgga tcgatgccct gctgcctcaa
    16021 acacaatgcg gcaaatgcgg atacgacggt tgccgaccct atgcgcacgc catggcgcat
    16081 gatggcgaag ccatcaaccg ctgccccccc ggcggcgatg cgggcataca cgcgctggcc
    16141 ggcctgctcg ggcggcccga aatacccttg aacacggcat gcggcgaaca tgccgcgccc
    16201 acggtcgccg tcatcgacga ggcccactgc atcggctgca cgctctgcat tcaggcctgc
    16261 cccgttgacg ccatcatggg cgccaacaag cgcatgcaca cggtgctggc ggatctttgc
    16321 acgggctgtg atctatgcgt ggcgccgtgc ccggtcgact gcatcaccat ggtaccagcc
    16381 ggtcgcgagt ggacgccgcg cctcgccacc gtggcgcgcc tgcgccatca gcgacgccag
    16441 aaaaggctgg ccgagcaaca ccccgaagcc tcggcactgg cctcccgccg cacactcgcc
    16501 aacaaggccc cggtcaccgc cgccgcccac gcggacgacg cgcgcaagca gcgtgtcatt
    16561 gccgacgcca tcgcccgagc ccgggcgcgc cgcgcgaaac cgacctagtg ccctgagccc
    16621 tgaactggtt cacgcattaa cacccaccaa acggcacact gcacagagtc tcccatgaac
    16681 gcggaaaaac gcgcgcgaat tttctcccgc ctgcgggccg ccaatccgga cccgacgacc
    16741 gaactcgaat acgccagcgt gttccagttg ctgatcgcgg tcatgctttc ggcgcaggcc
    16801 accgacaagt cggtcaacct cgccacacgt cgcttcttcc cggaccatgg cacgccgcaa
    16861 gcgctgctgg cgttggggga ggacgccttg gctgaattca tcaagaccat cggcctctat
    16921 cgcaccaagg cggccaacat catcaaaacc tgcgccatcc tgatcgaacg cgacaatggc
    16981 gaagtgccgg acgaccgcgc cgcactggaa tcgttgccag gcgtgggccg caagaccgcc
    17041 aacgtcgtgc tcaacaccgc gttcggacat cccacgatcg ccgtggacac ccacatcttc
    17101 cgcgtggcca accgcacggg cctcgcgccc gggaaaaccg tgctcgatgt ggaacgcagg
    17161 ctggagaaag tcgtgcccgc ggaattcgcc ctgaatgcgc accactggct cattcttcat
    17221 ggccgctacg tgtgtgtcgc acgcaaaccc cgttgccccc aatgcggcat cgccgatctg
    17281 tgcgagtacc ggcacaagac gccgccagac tgatccgaat gaccacccga cacaccaaaa
    17341 tcgaagtttg acgacgttgt caaatcaggc agctcaggtt tttctagggt ttaccctcaa
    17401 cctatgacaa acccgcatgg tattcaaggg ccgaacctcg catactgcga gctgttaaaa
    17461 atcccacata atgcatttcg atccaatggg ctggcatgag cgacctgata ctggaaaccc
    17521 accaccttac aaaggagttc cgcggattcg tggcggtcga tgacgtcaat ttccaggttg
    17581 ctcgcggaca tattcacgcg cttatcgggc ccaatggcgc cggcaagacc acttgcttca
    17641 acctgctgac caaattcctc gaacccacat ccgggcgcat catcttctcc gggcacgaca
    17701 tcacgggcga aaagccggcg cagatcgcgc gtcgcggcat catccgctcg ttccagatct
    17761 cggcggtgtt tccgcagctc acactgctcg agaacgtgcg cgtcggcctg cagcgggcca
    17821 ccggccagtc ctttcacttc tggcgcagcg acaagtgcct gaaccgcctg aacgacgcgg
    17881 ccatggcgtt gctcgcgcag gtcgatctcg ccagctacgc ccaggaacgc accgcgaacc
    17941 tgccctacgg gcgcaagcgc gcgctcgaaa tcgccaccac actggccatg gaacccgagc
    18001 tcatgctgct cgacgaaccc acacaaggca tgggccacga agacgtcgcc cgggtcacag
    18061 aactcatcaa gaaagtcggc gtcggacgca ccattctcat ggtcgagcac aacatgaaag
    18121 tcatctcctc catcgccgac cgcatcaccg tgctctcgcg cggctcggtg ctggccgaag
    18181 gggattacca agaagtctct cgcaacccgg aggtcatgca ggcctacatg ggcaccgagg
    18241 tcggcgaact tgaaggggca caggcatgag cgccgtactc gagatcaccg atctgcatgc
    18301 ctggtatggc gaatcgcatg tgctgcatgg cgtgaacatg caagtcaatc aaggcgaggt
    18361 cgtcaccctg ctcggccgca acggttcggg gcgtaccacc acgttgcgcg ccacactcgg
    18421 tctcacaggg tctcgcaagg gttcgatccg catcggcggc accgagtcca tcgcgctgcc
    18481 cacgtacaag atcgcccacc tgggtatcgg atactgcccc gaagagcgcg gcatcttcgc
    18541 gggcctgtcc tgcgaggaaa acctgttgct gcccccgccc gtcggcgcca gcacactggg
    18601 cggtggcatg tcgctggccg aaatctacga catgttcccc aacctgcaag aacgccggta
    18661 ttcacccggc acgcgcctct ctggcggcga acaacaaatg ctggccgtgg cgcgcatcct
    18721 gcgcactggc gccaacctgc tgctgctcga cgaaatctcc gaaggcctgg cgccggtcat
    18781 cgtgcaggca ctggcccgca tgatcacaac actcaaggcc aagggctaca ccatcgtcat
    18841 ggtcgaacag aacttccgtt tcgccgctcc gctggccgac cgcttctacg tcatagaaca
    18901 cggccaggtc gtagaacact tcaacgccgc cgagctcccc gaaaagcaag acgtcctcaa
    18961 cacactgctc ggtgtgtaaa cacgattcac gcgccggcga accggcataa caactccaag
    19021 gagagagatc catgaaattg cgcattctgt cggccgccct ggccacagcg ggactcggcc
    19081 tggccatggc cggccccagt cacgcggcgg gcatttccga taacgtcgtc cgcatcgggt
    19141 tcatcaccga catgtccggc gtgtactccg acatcgatgg caagggtggc gttgaagcca
    19201 tcaaaatggc catcgccgac gccggtggca agatcgatgg caagaaaatc gaactcttca
    19261 ccgccgacca tcagaacaag gccgacatcg ggtccgcgcg ggctcgtcaa tggttcgatg
    19321 aaaaacacat cgacatgctc gtcggcggca ccaactcggc cgtgaacctg gccatggcgg
    19381 ccgtcgccgc agaaaagcaa aagcctttct tcgccgtcgg tccaggctct tccaacctga
    19441 cgaactccgc ctgcacgcac tacaccatcc actacggcta cgacaccgtc gccctggcac
    19501 gcggcacggc caactacatg gccgagcacg gcgacaagac ctggtacttc ctgacggcag
    19561 actacgcctt cggccactcc ctgcaacgcg atgccaccaa ggtcatcgat gccgccggcg
    19621 gcaaggtggt gggcagcgtc aacgtgccgc tgggcacgtc cgacttctcc tcattcctgc
    19681 tgcaggcgca aagctccaag gcccaggtgc tggcgctggc caacgctggc ggcgattttg
    19741 tcaactccat gaaggcggcg caggaattcg gcatcaccaa gaccatgaag atcgccggcc
    19801 tgctggtgtt catcaatgac atccactccc tgggcctgaa ggccacgcaa ggcctgtacc
    19861 tgacgaacgc ctggtactgg gacaaagacg cggcgtcgcg caagttcgcc gagcgcttct
    19921 acaagaaaga acaccgcatg ccgtccttcc tacaggcggg cgattactcc gccgtgtcgt
    19981 tctacctgcg cggggtcaaa gccaccggca cggacaatgg caacgacatg atgaaatgga
    20041 tcaagtcgca caaggtcaac gactttttcg ccgacgatgg ctatgtgcgt gaagacggca
    20101 cgctcatcca caacatgtac ctgatgcagg tcaagacccc ggccgaatcc aagaagccgt
    20161 gggattacta caagttggtc aaggttctgc ctggcgacaa aatttatacg tcgctggcgc
    20221 aatcgacctg caagttcgtc aagaaataac ccgccgcaac caaggcctgc cccccgccac
    20281 atgggcggca ggccttaata ttgccaggag ccattcacat ttcgtcatgg attctcaaca
    20341 aatatgactg agctattcgg aattcccgtc caggcgcttc tgggccagtt gttgctggga
    20401 ctggtcaacg gggcgttcta cgccgtgctg tccctggggc tggcagtcat attcgggctg
    20461 ctcaacgtca tcaacttcgc ccatggcgcg ctgtacatgc tgggcgcctt cctggcctgg
    20521 atgggcctgc aatacctggg gctgaactac tgggtcatgc tgatcctcgc ccccatactt
    20581 gtgggcatct tcggcatcat catcgaacgc ctgctgctca aacacctcta caagctcgac
    20641 cacctctacg ggctgttgct cactttcggc ctgaccctgc tcatcgaggg gctctttcgc
    20701 agcctgtacg gcgtctcggg ccaaccttac tccacgcccg atttgttgca gggcggcgtg
    20761 aatctgggct tcatgtatct gcccatttat cgtgcctggg tcatcgtcgc ttcactcgtg
    20821 gtgtgtttcg ccacctgggc cgtcatcgag aaaacccgcc ttggcgcgct gctgcgcgcc
    20881 ggcaccgaga accccaaact ggtcgaagcc ttcggcgtca acgtgccact ggtgatcaca
    20941 ctcaccttcg gcttcggcgt ggggctggcg gggttcgcgg gcgtgcttgc cgcacccatc
    21001 ctgcaggttt cgccgctcat gggctccaac ctgatcatcg tggtcttcgc cgtggtcgtc
    21061 atcggcggca tgggttccat cctgggatcc atcgtcaccg gcctcggact gggcgtcatt
    21121 gaaggcctca ccaaggtatt ctggcccgaa gcgtccacca cggtcgtctt catc
//
LOCUS       DASTNK010000096        11101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_439579, whole genome shotgun sequence.
ACCESSION   DASTNK010000096 DASTNK010000000
VERSION     DASTNK010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11101
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439579"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4080
                     /locus_tag="VFR20_07420"
     CDS             <1..4080
                     /locus_tag="VFR20_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEU0230384.1"
                     /translation="GLHPRDMHRVVEVMHRLRANGNTLVVVEHDPQVMIAADRIMDIG
                     PGPGERGGRVVFDGTPDQLRGADTMTGNYLGGRLRVEAPRPLPVAAGTPRLVLDGVCA
                     HNLKQVTLSVPLGRLVCVTGVSGSGKSTLIQDVLYPAILKHMGRPTEAPGEYRHLLGV
                     EQIADVVMVDQTPIGKTARSNPASYVGAFDAIRKIFANAPLARERAYTPGTFSFNSGD
                     GRCPTCGGTGFEHVEMQFLSDVYLRCPDCDGKRFRPEILEVRAEHLGRSASIDQVLDM
                     TVHEALEFFSGLRDVQTGLAPLADVGLEYVRLGQPVPTLSGGEAQRLKLAGHLATAAR
                     SGIASAKVAKKGSLFLFDEPTTGLHFDDVARLMRAFRKLLAAGHTLLIIEHNLDVVRA
                     ADWIVDLGPEGGDAGGLVVGEGTPADLMDNPASHTGKALRDYTSAIVYEEQSQRAKAL
                     AEPRPDYADAGTPLQTLAARRRHNSIDIVNAREHNLKGVTVSIPRDTFTVVTGVSGSG
                     KSTLAFDILFNEGQRRYLESLNAYARAIVQPAGKPDVDAIYGIPPTVAIEQRTSRGGR
                     KSTVATMTEIHHFLRLLYVKLGTQMCPDCHVPVEPQKPEQIVAQLLRDHRGEHIGLLA
                     PLVTARKGYYTDLAKWAAGKGYTHLRVDGEFIPVSPWPRLDRYKEHTLELPVADLLVD
                     PADEPALRQAVHTALEHGHGSLSVLVGLTRDSTGTLAQRRAGDPEGTARQLHFSVKRA
                     CPNCGTSYPEPDPRMFSYNSKHGWCPDCFGTGLRLRGFDAEQTGEETVWNDWYEGEPE
                     PCPTCDGERLNPLARAFRWRDRSIAELAAQPVSDAQTFFTALVSHGREAEIARDILAE
                     IRGRLDFMQEVGLGYLALDRAAPTLSGGEAQRIRLAAQLGSNLQGVCYVLDEPTIGLH
                     SRDNQILLNALGKLEGHGNTLVVVEHDDDTIRRASHIIDIGPGAGTRGGRVVAQGSAD
                     DLMTEPESVTGRYLAHPLQHPLQPRREVRDDAAFIEVQGAHLHNLHNLRARLPVGRLS
                     VITGVSGSGKSTFARDVLLDNLARAVSQRKAPAWQGCTNITGWEHIDRVLEVDQTPIG
                     KTPRSCPATYVGFWDNVRRLFADARESRVRGWGPARFSFNTGEGRCPICEGRGLRTLE
                     MSFLPDVKVPCDACGGERFNQDTLSVTLRDKSAGQILKMEVDEAVGYFAAHSKVFRPL
                     QLMQDVGLGYLTLGQPSPTLSGGEAQRIKLVTELVKARLDDGVIKTGRASRLPHTLYV
                     LDEPTVGLSMADVEKLIHVLHRLVDAGNTVVVIEHNLDVIAEADWVLDLGPEGGREGG
                     RLVVAGTPETLVERRAVSHTGAALAPFMKAATDEVIHSV"
     gene            complement(4586..5455)
                     /locus_tag="VFR20_07425"
     CDS             complement(4586..5455)
                     /locus_tag="VFR20_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEU0230385.1"
                     /translation="MPEVCQVDMQRRFGGLERLYGPDALGTLGRAHVAVAGIGGVGSW
                     CAEALARSGVGALTLIDLDHIAESNVNRQLHALTDTLGAAKVQAMARRIHGINPECRV
                     NPVDDFVTPDNVREVLPVDLTLLIDCTDQIAAKTAMVVEAAARGLPIIVCGGAGGKTD
                     ALSLRAGDLSQATNDALLAKLRNTLRRHHGYPKASDHAGRPRRRIPKMGVRVLWFDQV
                     ARMPIAWMPGVKASEGPMARDDGAENGAPQGLSCAGYGSLVTVTAAMGMAAAGQSVHY
                     LVEGNYPGQAGPR"
     gene            5669..6232
                     /locus_tag="VFR20_07430"
     CDS             5669..6232
                     /locus_tag="VFR20_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEU0230386.1"
                     /translation="MEDSLAHWDNQSDLWVFGYGSLVWRPEFDFTEQRVALSHGYHRA
                     LCLWSRVNRGTPERPGLVFGLDVGGSCRGMAFRIPADDVHGTMRALWRREMPSGAYIP
                     RWLSCRTRQGVIQALAFVMNRNTDAYVRDLSRAQVVEIVRGAHGCYGPCVEYVLETAR
                     ALKLTGIHDKQLEAIVRELRGTLAGNE"
     gene            6267..6899
                     /gene="pdxH"
                     /locus_tag="VFR20_07435"
     CDS             6267..6899
                     /gene="pdxH"
                     /locus_tag="VFR20_07435"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEU0230387.1"
                     /translation="MSVADIRTTYQKGVLLEESVAPTPLEQFTRWFDEALAAHVVEPT
                     AMTLASVDQHGRPSARIVLLKGFDERGFVFFTNYESRKGREILANPHASLLFFWPALE
                     RQVRIEGEVTQVAGDESDAYYNSRPLGSRIGAWASPQSRPITHDELEARTRQFTESLG
                     EHPPRPPHWGGFRLRPDYLEFWQGRPSRLHDRLVFRRTGHDQWERERLAP"
     gene            6920..7444
                     /locus_tag="VFR20_07440"
     CDS             6920..7444
                     /locus_tag="VFR20_07440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU0230388.1"
                     /translation="MTARPADFDSAYFRSAMGRFATGVTVITCESPLDGQPMGLTISS
                     FNSVSLDPPLVLWTLTKSASSLQHFQSAERYVVHVLSASQLHLARRFAHGSQAARFQD
                     IAVDRTPDGTLMLNEPDCSAWFECFNAVRHDAGDHIIFIGQVERCKRQFSQPLVYHAG
                     DFDLTPSTEPLSSL"
     gene            7587..8702
                     /locus_tag="VFR20_07445"
     CDS             7587..8702
                     /locus_tag="VFR20_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0230389.1"
                     /translation="MTPDIDCVVLGAGVIGLAIARQLAESGREVLVIEAGDGIGSGVS
                     SRNSEVIHAGIYYPRGSLKARLCVEGRHRLYTYCVERGIPHRQTGKLIVAATPGQSAQ
                     LDDIAARAHANGVDDLYRIDRATARALEPELECHAALVSPSTGIVDSHALMLALQGDA
                     ERRGAQCVFRTRFHAGRVLPSEGFELRFDGEESMALTARCVVNATGLWAPDTARRLTG
                     QPEALIPTAYLCKGSYFSLAGKSPFSRLIYPMPDEAGLGVHLTLDLGGQARFGPDTQW
                     IDTEDYTVDPHRADVFYAAIRRYWPGLADGALQPAYAGIRPKITGPGTPAADFVISGL
                     AAHGVPGLVNLFGMESPGLTACLAIAGAAVAALGDGA"
     gene            8830..9696
                     /gene="purU"
                     /locus_tag="VFR20_07450"
     CDS             8830..9696
                     /gene="purU"
                     /locus_tag="VFR20_07450"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEU0230390.1"
                     /translation="MTHDAPTHDFILTLSCPDRIGITHAVSGWLLGLHGNIMDTQQFG
                     DSETRRFFLRAHFRLPEPSDADALRASFSTVAERFDMDARFHDASRRARLLILVSRQA
                     HCLNDLLFRMRSGDLRAEAAAIVSNHQDCAELAASYGVPFHHLPVTPDTRDAQESRIL
                     EIAQRERAELVVLARYMQILSADLCGKLANQAINIHHSFLPGFKGARPYHQAHARGVK
                     IIGATAHYVTPDLDEGPIIEQDVQRVDHTAGPEELARVGRDIESRVLARAVRSHVEHR
                     VLPNGIRTVVFR"
     gene            complement(9768..10688)
                     /locus_tag="VFR20_07455"
     CDS             complement(9768..10688)
                     /locus_tag="VFR20_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0230391.1"
                     /translation="MRNGIPNLGALQAFEATARLGSFSRAAEELSLTHSAVYRQVTGL
                     EERLGVELFTRVRRRVALTEAGAEYAGRVRHHLEQLEKDTFGLVSRARLGNSLHLAVL
                     PTVATTWLIPRLPDFAARHPDIAVSLSVQTLPFQFNDHPYDAAIYHATQAWAHTRGIK
                     LFEEHSLVPVCGATMAARVAGRVACMADLTHLHMASRPDAWRQWYREQGLDYPPSVGA
                     GPRYELFSMTLAAVCAGVGVALMPRLFVQEALAAGELCVPVKGGLPVDEAYYFGYPAD
                     SEPSAALRAFESWLDAAAEADRRGASVGAA"
BASE COUNT         1969 a         3708 c         3538 g         1886 t
ORIGIN      
        1 ggcctgcacc cgcgcgacat gcaccgcgtg gtcgaggtca tgcatcgcct gcgtgccaat
       61 ggcaacacgc tggtggtggt ggagcacgac ccccaggtga tgatcgcggc cgatcgcatc
      121 atggacatcg gcccgggtcc gggagaacgc ggcgggcgcg ttgtgttcga cggcacgccg
      181 gaccagttgc gtggcgccga caccatgacg ggcaactacc tgggcggacg cctgcgcgtc
      241 gaggcgccgc gacccctgcc ggtggcggcc ggcacgccaa ggctggtact ggatggagtc
      301 tgtgcccaca acctgaagca agtgacgctg tcggtgcccc tgggccgcct ggtctgtgtg
      361 acgggtgtat caggatccgg aaaatccacg ctgatccagg atgtgctgta tcccgccatc
      421 ctcaagcaca tggggcgccc gaccgaagcg cctggcgaat atcggcatct tctgggggtc
      481 gaacagatcg ccgacgtggt catggtcgat cagaccccca taggcaagac ggcgcgctcg
      541 aatcccgcaa gctatgtcgg cgcattcgac gccatacgca agatcttcgc caacgcgccg
      601 cttgcgcgcg agcgcgccta cacgcccggg acgttcagct tcaacagcgg cgatggccgc
      661 tgccccactt gcggcggcac cgggttcgag cacgtggaaa tgcagttcct gtccgacgtg
      721 tacctgcgct gcccggattg cgacggcaaa cgcttccgtc ccgaaatcct cgaggtccgt
      781 gccgagcatt tgggtcgcag cgcgtccatc gaccaagtgc tcgacatgac cgtgcacgag
      841 gctctggagt tcttcagcgg cctgcgcgac gtgcagacgg gtctggcgcc cctggccgac
      901 gtgggcctgg aatacgtgcg gttgggccaa cccgtgccga cgttgtcggg cggcgaagcg
      961 cagcgcctga agctcgccgg ccacctggcc acggccgcgc gcagcggcat cgcgtcagcc
     1021 aaggtggcca agaagggcag cctgttcctg ttcgacgaac cgaccacggg cctgcacttc
     1081 gacgacgtcg cgcggctcat gcgcgccttc cgaaagctgc tggcggccgg ccacaccctg
     1141 ctcatcatcg aacacaacct ggatgtcgtg cgggctgccg actggatcgt cgacctgggc
     1201 cccgaaggcg gtgacgccgg ggggctcgtg gtgggtgaag gcacccccgc cgacctgatg
     1261 gacaaccccg cgtcgcacac ggggaaggca ctgcgcgact acaccagcgc catcgtctac
     1321 gaggaacaat cgcagcgcgc caaggcgctg gccgagccgc gacccgacta cgccgacgcg
     1381 ggcacaccac tgcaaaccct cgcggcgcgc cgacgccaca acagcatcga catcgtcaac
     1441 gcgcgcgagc acaacctcaa aggggtgacg gtgtccatcc cgcgcgacac cttcaccgtg
     1501 gtgacgggcg tatcgggatc cggcaaatcc accctggcct tcgacatcct gttcaacgaa
     1561 ggccagcgac gttatctcga gtcattgaac gcctatgcgc gcgccatcgt gcagccggcc
     1621 ggcaagccgg acgtggacgc gatctacggc attccgccca cggtcgccat cgagcagcgc
     1681 acgagccgcg gcggccgcaa gtccaccgtc gccaccatga cggaaatcca ccacttcctg
     1741 cgcctgctgt atgtgaagct tggcacgcag atgtgcccgg attgccatgt gccggtcgaa
     1801 ccacagaaac ccgaacagat cgtcgcccag ttgctgcgtg atcaccgagg tgagcacatc
     1861 ggcctgttgg cgccgctggt cacggcccgt aaaggctact acacggatct cgccaaatgg
     1921 gcggccggca agggctacac acacctgcgc gtggatgggg agttcatccc cgtgtcgccg
     1981 tggccgcgtc tggaccgtta taaagagcac accctcgagc tccctgttgc cgacctcctg
     2041 gtcgacccgg cggacgaacc cgcgctgcga caagccgtgc atacggccct ggagcatggc
     2101 cacggtagcc tgagcgtgct ggtggggttg actcgcgaca gcaccggcac gctggcacag
     2161 cgccgcgcgg gtgacccgga gggcacggcg cgtcaactgc atttctcggt caagcgggcg
     2221 tgcccgaact gcggcaccag ctacccagaa cccgacccgc gcatgttctc gtacaactcc
     2281 aagcacggct ggtgtccgga ttgtttcggc accggactgc ggctgcgcgg cttcgatgcc
     2341 gagcaaacgg gtgaggaaac cgtctggaac gactggtacg agggcgagcc agagccctgc
     2401 cccacctgcg acggcgaacg tctcaatccg ttggcacgcg cgttccgctg gcgcgaccgc
     2461 tcgatcgccg agctcgccgc gcagcctgtc tcggatgcgc agaccttctt caccgccctg
     2521 gtcagccatg gacgggaagc ggaaatcgcg cgcgacatcc tggcggagat ccgcgggcgg
     2581 ctggatttca tgcaagaggt ggggctgggc tacctggcat tggaccgggc cgcccccacg
     2641 ttgtcaggcg gcgaggcgca acgcatccgg ctggccgcgc aactgggctc gaacctgcag
     2701 ggtgtgtgct atgtcctgga cgagcccacc atcgggctgc attcgcgcga caaccagatc
     2761 ctgctgaacg cgttgggaaa actggagggt cacggcaaca cattggtcgt cgtcgaacac
     2821 gatgatgaca ccatccggcg ggcgtctcac atcatcgata tcgggccggg tgctggcacg
     2881 cgtggtggcc gggtcgtggc gcagggcagc gccgacgacc tgatgacgga acccgaatcg
     2941 gtcacggggc gctacttggc gcacccgctg caacacccct tgcagccacg ccgagaggtt
     3001 cgggatgatg cggctttcat cgaagttcag ggtgcgcatc tgcacaatct tcacaacctg
     3061 cgcgcgcgcc tgcccgtggg ccggctcagc gtgatcaccg gggtgtcggg ttccggcaaa
     3121 tccacgtttg cacgcgacgt gctgctcgac aatctcgccc gcgcggtgtc gcagcgcaaa
     3181 gctccggcct ggcagggctg cacaaacatc acgggctggg aacacataga tcgcgtactg
     3241 gaagtggacc agaccccgat aggcaagact ccgcgctcat gcccggccac ctatgtgggt
     3301 ttctgggaca atgtgcgccg tctgttcgcg gacgcgcgtg aatcgcgtgt gcgcggctgg
     3361 gggccggcgc gcttttcgtt caacacgggc gaggggcgct gccccatttg cgaaggccgt
     3421 ggcctgcgca cgctggaaat gagtttcctg ccggacgtga aagtgccttg cgacgcctgc
     3481 ggcggagagc gcttcaatca ggacacgctg agcgtcacct tgcgcgataa aagcgcgggc
     3541 cagatcctga aaatggaggt ggacgaggcg gttggttatt tcgcggcgca ctcgaaggtt
     3601 ttccgtccgc tgcaactgat gcaggatgtg gggctggggt acctgacgct cggtcagcct
     3661 tcgccgacgc tgtcgggtgg cgaggcgcag cgcatcaagc tggtgacgga actggtgaag
     3721 gcgcggctgg acgatggcgt gatcaagacg gggcgcgcgt cacgcctgcc tcacacactg
     3781 tatgtgctgg acgagccgac ggtggggttg tccatggccg acgtcgagaa gttgatccat
     3841 gtgttgcacc ggctggtgga tgcgggcaat acggtggtgg tgatcgagca caatctggat
     3901 gtgatcgcag aggccgactg ggtgttggac cttgggccgg aaggcggtcg tgagggcggg
     3961 cgactggtgg tcgcgggcac gcccgaaacg ctggtcgagc ggcgggccgt gtcgcacact
     4021 ggcgcggcgc tggcgccgtt catgaaggcg gcgacggacg aagtgattca ttcagtctga
     4081 tcgtggcggt gccaagcttg tcgcgcagac ttggtatctg aaggcgctgt ccatcggggt
     4141 ctggggtggc tccggcacgg catgcggggg agtcaactga cccatcgccc tgcatggact
     4201 gtgtggcagc gcatacctct atgcattccg gtatcgtagc tgcagggtgg cagaggcggt
     4261 gcgggccggc gtttcggcca gcgccggggc tgacgtaggg aaggcggggg atttcgtcgg
     4321 gcgatgtctg agttgtgcct cgcgggtacg cgagacacaa cgagtttcgc ccgacgcccg
     4381 cctggacgag gcagacccgg gcagtcgggg cgcgcagcgc cacaactgct ggacgcgccg
     4441 gcccgcaccg cctctgccac cctgcagcgt gttaaatact gtccagtggc gtgtcaaaca
     4501 ccatacagcg tgttaaacaa cagacaccgg agtgaaacag actcaccccg gccaaaatcg
     4561 gttcaagcac tgacgcaaca cgggttcaac gcggaccggc ctgcccaggg tagttgccct
     4621 cgaccaggta atgcaccgac tgaccggccg ccgccatgcc catggctgcg gtgacagtca
     4681 ccaaggaccc ataccccgcg caggatagcc cttgtggcgc accattctcc gcaccgtcat
     4741 cccgagccat tgggccctcg gaggccttca cacccggcat ccaggcaatg ggcattcgcg
     4801 ccacctgatc gaaccacagc acccgcacgc ccattttcgg gatacgccgg cgtggtcgac
     4861 ctgcgtggtc ggacgccttg gggtagccgt gatggcgccg caaggtgttg cgcagcttgg
     4921 cgagcagcgc gtcattggtc gcctgggaca ggtcaccggc gcgcaggctg agcgcatcgg
     4981 ttttgccgcc tgcgccgccg catacgatga tgggcagtcc gcgtgccgcc gcctcgacga
     5041 ccatggcggt cttggcggcg atctgatcgg tgcagtcgat gagcagggtc aggtcgacgg
     5101 gcaacacttc gcgcacgttg tcgggggtga cgaagtcgtc gaccgggttc acccggcatt
     5161 cgggattgat gccatggatg cgccgcgcca tggcttgcac cttggcggcg cccagggtgt
     5221 cggtcagggc gtgcaactgg cgattgacgt tggattcggc gatatggtcg agatcgatga
     5281 gcgtcaatgc gccgacgccg gatcgcgcca gcgcctcggc gcaccaggag ccgacgccgc
     5341 ctatgcccgc gaccgccacg tgggcgcggc ccagtgtgcc gagggcatcg gggccgtaca
     5401 gacgttccag tccgccaaag cggcgctgca tgtccacctg acagacttca ggcatgaggg
     5461 ggttccttgc aatatcttga aaatcgcgac acagggcgaa gggattcgag caaacgagtt
     5521 cgagtttcca gcgcggcggg cttaaaatga cacaagcacc cacaagccac cattacccat
     5581 catcgtgacc gacacgttgt cttcccgtcc atgccctccc gccgcatcga gctttcgcct
     5641 gctcaccgac gcgcaacgcc aggcgtccat ggaagacagc ctggcacact gggacaatca
     5701 aagcgatctc tgggtgttcg gctacgggtc gctggtttgg cggcccgagt tcgattttac
     5761 cgaacagcgt gtggcgctgt cgcatgggta ccatcgggca ctgtgcctgt ggtcgcgcgt
     5821 gaaccgtggc acgccggaac gcccgggcct ggtcttcggc ctggacgtgg gcgggtcgtg
     5881 ccggggcatg gccttccgca ttcccgctga cgacgttcac ggcaccatgc gggcgctgtg
     5941 gcggcgcgaa atgcccagcg gggcctacat tcccaggtgg ctgtcgtgcc gcacgcggca
     6001 aggcgtgatc caggcgctcg ccttcgtcat gaaccgcaac accgacgcct acgtgcgcga
     6061 tctgtcacgc gcccaggtgg tcgagatcgt gcgcggcgcg cacggctgct acggcccatg
     6121 tgtcgaatat gtgctggaaa cggcgcgcgc cctgaaactg accggcatcc acgacaaaca
     6181 gctcgaagcc attgtgcgcg agctgcgggg cacgcttgcc gggaacgagt aaactcatac
     6241 cctggtcatt ctttcattcc cgcctcatgt ccgtagccga tatccgcact acttaccaga
     6301 agggcgtcct gctcgaagaa tccgtggcgc caacgccgct ggaacaattc acccgctggt
     6361 tcgacgaagc cctggcggcc catgtcgtcg aacccaccgc catgaccctg gccagtgtgg
     6421 accaacatgg ccgcccgtcc gcacgcatcg tgttgctcaa aggcttcgat gagcgcgggt
     6481 tcgtgttctt caccaattat gaatcacgca agggccggga gatcctcgcc aaccctcacg
     6541 ccagtctgct gttcttctgg cccgcgctgg aacgacaagt gcgcatcgag ggcgaagtaa
     6601 cccaagtcgc cggcgacgaa tccgacgcct attacaacag ccgcccgctg ggttcgcgca
     6661 tcggcgcatg ggcctcgccg caaagccgcc ccatcacgca cgacgagctt gaggcgcgca
     6721 cccgtcaatt caccgagtcg ctgggcgagc acccaccgcg cccgccgcac tggggggggt
     6781 tccgattgcg gccggactac ctggagttct ggcagggtcg gccttcgcgc ctgcatgacc
     6841 ggctggtttt tcgccgcacg gggcacgatc aatgggaacg cgagcgcctg gccccttgag
     6901 ccggaaccgc gcaataccca tgacggcccg acccgctgat ttcgactccg cctatttccg
     6961 ctccgccatg gggcgcttcg ccaccggggt gacggtcatc acgtgcgaat cgccactgga
     7021 cggccagccc atggggctca ccatcagttc gttcaactcg gtgtccctcg atcccccgct
     7081 ggtgctgtgg acactgacca aatccgcatc gtccctgcag cacttccaaa gcgccgaacg
     7141 ctatgtcgtg catgtgctgt cggcttcgca actccatctg gccaggcgtt tcgcgcatgg
     7201 cagccaggcc gcgcgtttcc aagacatcgc cgtcgaccgc acaccggacg gcaccctgat
     7261 gctgaacgag ccggactgct ccgcctggtt cgaatgcttc aacgccgtgc ggcacgatgc
     7321 cggagatcac atcatcttca tcggccaggt cgaacgttgc aagcgccaat tcagccaacc
     7381 actggtctat cacgcgggcg atttcgacct gaccccgtcc accgagccgc tctccagtct
     7441 ctgaccggat cacccgccac cacctcgaag catttccgaa tgggcacagt tttcatattc
     7501 cgggcggaca ttccgtccat ccttcaaatg ggcaccccgc cttcccgtcg cggcccgatt
     7561 ccatggcgcg ccggaggccg acccgcatga caccggacat cgactgtgtc gtcctgggtg
     7621 cgggcgtcat cggcctggcc atcgcccgcc aactggcgga atccggccga gaggtcctgg
     7681 tgatagaggc cggcgacggc ataggctccg gcgtgagttc gcgcaattcg gaagtgatac
     7741 acgccggcat ctactatccg cgcggcagcc tgaaggcgcg tctgtgtgtc gagggccggc
     7801 accgcctgta cacctattgc gtcgagcgcg gcatccccca ccgccagacc ggcaagctca
     7861 tcgtcgcggc gacacctggg caatcggcac aactcgatga catcgccgca cgggcacatg
     7921 ccaacggcgt ggacgacctg taccgtatcg accgggccac cgcccgcgca ctcgaacccg
     7981 aactggaatg ccacgcggcg ctggtctcgc catccaccgg catcgtggac agccacgcgc
     8041 tgatgctggc cctgcaaggc gacgcggaac gccgtggcgc gcaatgcgtg ttccgcacac
     8101 gcttccacgc ggggcgggta ctgccttccg aaggattcga gctgcgcttc gacggtgaag
     8161 aatccatggc cttgacggcg cgttgtgtcg tcaacgccac cgggctgtgg gcgcccgaca
     8221 ccgcgcggcg cctgaccggc cagccggagg cgctcattcc caccgcctat ctgtgcaagg
     8281 gcagttattt cagtctggcc ggcaaatccc ctttttcgcg gctcatctac ccgatgccgg
     8341 acgaagcggg gctgggtgtg catctgacgc tggatctggg tggccaggcg cgtttcggcc
     8401 ccgacacgca gtggatcgat accgaagact acacggtcga cccgcaccgg gccgacgtgt
     8461 tctacgcggc catccgccgt tactggcccg gcctggccga cggcgcgctg caacccgcct
     8521 acgccggcat ccgtccgaaa atcacgggcc ccggcacgcc agcggccgat ttcgtcatct
     8581 cggggctggc ggcccatggc gtgccgggcc tggtcaatct gttcggcatg gaatccccgg
     8641 ggctgaccgc ctgcctggcc atcgccggtg ccgcggtcgc agcgctgggc gatggcgcat
     8701 aacgccggat gacggtgcgt cgatatcccc accgttgaca catttcattc ccagagcacc
     8761 ccacttatca ggctgtccga atttccgccg ccacctctct ccgcccccaa tcgcctccat
     8821 cacaacacca tgacgcacga cgctccgacc catgacttca tcctgacctt gtcctgcccc
     8881 gatcgcatcg gcatcacgca cgcggtgtca gggtggttgc tgggcctgca tggcaacatc
     8941 atggacaccc agcaattcgg cgacagcgaa acgcggcgct tcttcctgcg tgcgcacttc
     9001 cgcctgcccg agcccagcga tgcggacgcg ttgagggcca gtttctcgac tgtggcggag
     9061 cgcttcgaca tggacgcgcg attccacgac gccagccggc gggcgcgcct gctcatcctg
     9121 gtcagccgcc aggcgcattg cctgaacgac ttgctgttcc gcatgcgtag cggtgatttg
     9181 cgcgccgagg ccgcggccat cgtgtccaat caccaggatt gcgccgaact ggcggcatcc
     9241 tatggtgtcc cgttccatca cctgccggtg acgcctgaca cccgcgatgc ccaggaaagt
     9301 cgaatcctgg agatcgcgca acgcgagcgc gccgaactgg tggtgctggc gcgctatatg
     9361 cagatcctgt ccgccgatct gtgcggcaag ctggccaacc aggccatcaa catccaccac
     9421 agcttcctgc ccggcttcaa aggcgcccgc ccctatcacc aggcccacgc gcgcggggtg
     9481 aagatcattg gcgcgacggc ccattacgtc acgcccgacc tggatgaggg tcccatcatc
     9541 gaacaggacg tgcagcgcgt ggaccacacc gccgggccgg aggaactggc gcgcgtgggc
     9601 cgcgacatcg aatcgcgggt tctggcgcgc gccgtgcgca gccatgtcga acaccgggtg
     9661 ctgccgaatg gcatccgtac ggtggttttt cgttaaagcg cgagcctgaa acccgtctga
     9721 agagaaagga gtccggacgc tctggctttg aaacaagccc tgatcgctca agcagcgccg
     9781 accgaagcgc cccgccgatc agcctccgca gcggcgtcaa gccacgactc gaacgcccgc
     9841 aacgccgccg agggctcgct gtccgccgga tagccgaagt aatacgcctc atccaccggc
     9901 aggccgccct tgactggcac gcacaactcg cccgccgcca gggcctcctg gacgaacaag
     9961 cgtggcatca gagccacgcc cacgcccgcg cacaccgccg cgagcgtcat gctgaacaac
    10021 tcgtagcgcg gtccggcgcc cactgagggc gggtagtcca atccttgttc ccgataccat
    10081 tggcgccagg cgtcggggcg cgaggccata tgcagatggg tcaggtcggc catgcaggca
    10141 accctgcccg ccacgcgcgc ggccatggtc gcgccacaga cggggaccag ggaatgctcc
    10201 tcgaacaact tgatgccgcg cgtgtgcgcc cacgcctgcg ttgcatgata gatggcggcg
    10261 tcatacgggt ggtcgttgaa ctggaacggc aatgtctgca ccgacaggct cacggcgata
    10321 tctggatgcc gcgcggcgaa atccggtagc cggggaatca gccaggtcgt ggccacggtc
    10381 ggcagcaccg ccaggtgcag gctgttgcca agccgggcac gcgacaccag accgaacgtg
    10441 tctttttcca gttgctccag atggtggcgg acgcggccgg cgtattcggc acccgcctcg
    10501 gtgagcgcca cccggcgccg cacacgcgtg aacaattcca cgcccaaacg ctcctccagc
    10561 cctgtgactt ggcggtacac ggcgctgtgg gtcagcgaaa gctcttcagc cgcccgggaa
    10621 aaagagccca ggcgggcagt ggcctcgaag gcctggagcg cgcctagatt ggggatgccg
    10681 tttctcatga tcgcggttgc aacagtctgt tggtgggatg gagcctagtg tcctaaattg
    10741 gaagtccggt gaataaatca tccgaatatc gacgccatgc ggcgttgcaa atcctcgcga
    10801 tagcaaaggc tatcgctgcg gtttgcgccc tgcctgacgc cgatctcgat tgatttattt
    10861 ttcaacgaac ctccaactca ggacactgga tgagccggcg tagaatgaac aagcccccag
    10921 tggcaccggg cgtcttgcga atcatgcaag acattgtgca attttatcaa ttgtcttgtg
    10981 catcttttgc acattatcct tgctgaatca tgacccatca caccacgaca ccctcttccc
    11041 ggctcggcgg ccacatcctc gtcgatcaac tcgtcgccca gggcgtggag cacgtattct
    11101 g
//
LOCUS       DASTNK010000097        11743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1550204, whole genome shotgun sequence.
ACCESSION   DASTNK010000097 DASTNK010000000
VERSION     DASTNK010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11743
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550204"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /gene="rpsG"
                     /locus_tag="VFR20_07460"
     CDS             complement(<1..413)
                     /gene="rpsG"
                     /locus_tag="VFR20_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU0230392.1"
                     /translation="MPRRREVPKREILPDPKFGSVDLAKFMNVVMLSGKKAVAERIVY
                     GALDQIQAKTGKEPIEVFNLAINNIKPVVEVKSRRVGGANYQVPVEVRPVRRLALAMR
                     WLREAAKKRGEKSMDLRLAGELLDASEGRGGAMKKR"
     gene            complement(545..922)
                     /gene="rpsL"
                     /locus_tag="VFR20_07465"
     CDS             complement(545..922)
                     /gene="rpsL"
                     /locus_tag="VFR20_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU0230393.1"
                     /translation="MPTISQLVRKPREVSRASSKSPALENCPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEVISYIGGEGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGSL
                     DLQGVKDRKQARSKYGAKRPKKA"
     gene            1318..2016
                     /locus_tag="VFR20_07470"
     CDS             1318..2016
                     /locus_tag="VFR20_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0230394.1"
                     /translation="MHLALLEDDPDQARLIGHMLAEAGHECASFASGRELLAALQKSE
                     PFDLLLLDWEVPDLTGLEIVRWVRAHMGYTIPVIFVTNRIQEADLVEGLRAGADDYMT
                     KPISRVELAARVAALLRRGHPQAMADTAFEFGPYTIDPAEGVILLNGEKVPLAPKEFD
                     LALLFFRNPGRLFSRDVLSTTVWNRDIPATSRTLDTHLSNMRQKLALRPEHGFRLTSS
                     YALGYRLEAVNDNI"
     gene            2034..3131
                     /locus_tag="VFR20_07475"
     CDS             2034..3131
                     /locus_tag="VFR20_07475"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEU0230395.1"
                     /translation="MPLLRLLSALALALLGAMPAVASAQAAGAQGTDFLYNAVSGDTL
                     IGLSSRYTLSPDHWRTLRQINHVADPAKLSIGKLIHIPFALIPEVASTANVTHVSGLA
                     QIDGRPLAVGQKLGEGSIVTTGADGFATLQLTDASVLTVPASSTLAIRRLRSFKGTGL
                     VDAIMALKHGGIESSVAPRHTGVGRFEIRTPLSITGVRGTHLRVRNNARGAQSEVVRG
                     RALVASSRQDKTATLHADQGTGVAPDGHILAIRKLLPAPALPKPERGGAAGWRMAFPA
                     VPGASAYLVRVATDAAVTRLVSTHTFSKPDVTFNAPGAGTFYVAVRAVDPDGIEGNDA
                     VRSFPGAPVLTSSDGLPVLSGSGRPILLSRY"
     gene            3142..5145
                     /locus_tag="VFR20_07480"
     CDS             3142..5145
                     /locus_tag="VFR20_07480"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEU0230396.1"
                     /translation="MLTAGGGARTPFWGNLDRRIRLEWVLVLMALSLFTAALSFFSHE
                     VGLTRLDHTFYDNMLATATSQPARDDIAIVAIDDASIAQLGYWPWRRRVHADLLRHLR
                     GAKAIGMDIVFSDPNPAYPGDDTLLAQAISADGNVVLPMLIDGGRALTPIEPLASAAK
                     ALGYINVKPDNDGVVRSMRPRQTLASGKTVTHFALALLRVAGATPRIPSWAGSSNLLI
                     PYAGPPGHFTLYPYAAVLDGRVPASAFKGKYVIVGAWGTGLGDTFPTPLTLQGKPMAG
                     VEILANALQAALTGDWIRTPPRGLAALLSSLPVLLLCLFLRHLSPRRGFFVAVLALLL
                     IFLADALLLHFARVWVAPAASLIGVILTGPVWSWRSQEAALQHIDRELETLHAEDSRI
                     GARQPCGPLHDGSLPARITLLHGAIGQIRRLRTSELAAQRQREETLRFISHDMRAPQN
                     SILALASLQSEPDTRLPEEQLLRRINQHAGRTLALVDGFIQLARAESMALQTRLIDLA
                     DLIAQLRDDYWALAKKHDITLDCGHDVSPGGAIVDGDESMMARALGNLVDNAIKYSAA
                     STTVACRLSRQGASWRITIADQGRGIAPDKLPELFTPFRRIDENTTGNPAGSGLGLAF
                     VKTVVTRHGGTISAASEPGRGATFTVLLPAVDADADADDRHGA"
     gene            complement(5519..7042)
                     /locus_tag="VFR20_07485"
     CDS             complement(5519..7042)
                     /locus_tag="VFR20_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEU0230397.1"
                     /translation="MSEILAHLSIGFDVASSWSNLLVAALGSFFGTMVGVLPGLGPIN
                     GVAMLIPIAFAMNLPPETALILLAAVYVGAEYGGRITAILINVPGEASAIMTTLDGYP
                     LARKGLANVALSLSAWSAFFGSLVSVIGITLLAPLLAGWALAFGPAEYFVLMVFSFCC
                     LSSLLGSEPVKGALSALLGLAIAIVGVDANSGVYRYTFGSVHLFDGIQFVVVVIALFA
                     VAELLEMLERVTAGHKVEVDSSGRKLFNVSEWMFTWWTVVRSAVLGFFVGVLPGAGAS
                     VASAVAYSNEKRLCESRDPNAKFGKGDLRGVAAPEAAATASAVGAFVPMLTLGVPGSG
                     TTAVMMGALTLYNLTPGPVLFDQQPQLVWGLVASLFIANIMLFLMNVPMVRVFSKVLQ
                     VPGWLMVPGILAISYIGVYAINAGTFDLAMAAILGGIGYLLRKTGIPMAPMVLGVVLG
                     DMMEQNLRRALSMTNGELHVLFDSNIAKGLWIVSAATVVLPPLLRYLRKRRAVVSIA"
     gene            complement(7039..7476)
                     /locus_tag="VFR20_07490"
     CDS             complement(7039..7476)
                     /locus_tag="VFR20_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEU0230398.1"
                     /translation="MTDRILGITTIAFAVLMAWFGRTIEAPFSYEPVGPRAFPMLLAL
                     SIGLCGLWLAFRGRHTAERNPPGANSRIALMVVYAIAYALLFDLLGFIIATTLVTILV
                     GRLFGGGWAKVTAGGVIMSVSFYFLFDRALDVVLPLGLLERVS"
     gene            complement(7481..8443)
                     /locus_tag="VFR20_07495"
     CDS             complement(7481..8443)
                     /locus_tag="VFR20_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0230399.1"
                     /translation="MLLGTMVFALNGAAAAQEPQRPECIAPAKPGGGFDLTCRLAQEG
                     LKATGQLKQPMRIIYMPGGIGAVAYNHIVAQDPAAGNKIVAFSGGSLLNLAQKKFGKY
                     DANAVRWLAAVGSDFGVAIVRKDSPYKTLQDLMTALKADPSKIVLGAGGTVGSQDWMK
                     AALTAKAAGVDYKKMRFVAFEGGGEANTALRGGHIQAYMGDAAEAFTMMKGGSPIRVL
                     AVFADKRLPGEMSGIPTAKEQGFDIEWPIIRGFYVGPKVTDAEYDWWVRAFSDMQKDP
                     KFDALRDKLGLFPFRMTGNELDAYVRQRVKSYAELADSFGLLRK"
     gene            complement(8657..10090)
                     /locus_tag="VFR20_07500"
     CDS             complement(8657..10090)
                     /locus_tag="VFR20_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014906141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0230400.1"
                     /translation="MDTTEFGRSRGLKSILLWSLVPALVLFLTVALFLSNQRLRAQIN
                     FAYDRSLTGALRAIDHQISTASGGLSVELPYLMLQFFETAANERVYFRVMTEDGLADV
                     GNQRLPLPDGTLISNVPVFYTTRYLGVDVRIAAMARRMDPPLYGHASGRVVVLVGEDV
                     DTREAFINSMLWRSVQRDLAAVLLVILIVVLAVLFAVRPLTRLRQEVAGRGIDDLRPM
                     EARGLPAEVAPLVTAINLHMDRYARQASLQNQFLDDASHQLRTPLTVLRAQLDYALRE
                     SDPQELRAVLRAMREGLDRSVRTANQMLALARVRDFQALEEGGAEFTDLAKLAAAVAS
                     DMYPTARTRRLDLGLERPDEAVLVRGPEWLLREAVVNVLDNALRYTPTGGVVTLRVRS
                     EAGRALIQVDDDGPGMSVEDIAKAGVRFRRGKAGKNTSGAGLGLAIVHAIVQQLRGEF
                     RLESEGRGCRATLVFTLVSGQKSGEPA"
     gene            10111..10809
                     /locus_tag="VFR20_07505"
     CDS             10111..10809
                     /locus_tag="VFR20_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0230401.1"
                     /translation="MRILLVEDESELARWLTRSLARHGGFIADWADDAELAERRMAVE
                     EFDAVVLDLGLPGMDGQTFLTRLRARDDRTPVLVLTARDSLAERVHTLHHGADDFLLK
                     PFVIEELEARLVALIRRSRGRHHPRLSLGDLALDTAAQRFTFGGQPLPLSPREFAALK
                     VLIQKSGEPVSKAQILDRINSDDHDINPEAVEVLVHRLRKKLAGTPVRIATLRGMGYS
                     LELDPGADAPTTGS"
     gene            complement(10913..>11743)
                     /locus_tag="VFR20_07510"
     CDS             complement(10913..>11743)
                     /locus_tag="VFR20_07510"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937409.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU0230402.1"
                     /translation="ARSPKDAGMLADITGTVSFGKDTKGKQRLVITDLEGVNHEFLIP
                     KEKQVLVHDGQVVNKGEMIVDGPADPHDILRLQGIERLATYVVNEVQDVYRLQGVKIN
                     DKHIEVIVRQMLRRVNIADAGDTSFITGEQVERSELLNENDRVEADGKIPATYENVLL
                     GITKASLSTDSFISAASFQETTRVLTEAAIMGKRDDLRGLKENVIVGRLIPAGTGMSY
                     HIARKDKEAHEAAERQTARAMANPFEEMPVTAATEETTEVVPAPDHSEPHVDNGPTEA
                     "
BASE COUNT         2188 a         4004 c         3544 g         2007 t
ORIGIN      
        1 cgtttcttca tcgcgccgcc acggccttcg gaggcatcca gcagttcgcc ggccagacgc
       61 agatccatcg acttttcacc gcgcttcttg gcggcttcgc gcaaccagcg catggccagg
      121 gccaggcggc gcacggggcg cacctcaacc ggcacctgat agttcgcacc gccgacacgg
      181 cggctcttga cttcgaccac gggcttgatg ttgttgatcg ccagattgaa gacttcaatg
      241 ggctctttgc cagtcttggc ctggatctgg tccagcgcgc cgtagacgat gcgctccgca
      301 acggctttct tgccggacag catcaccacg ttcatgaatt tggcgaggtc cacgctgccg
      361 aacttgggat cagggaggat ttcacgtttg ggtacttcgc gacgacgagg catttttcaa
      421 cttccttgtg acagttcagt tttaccgcgc attgcgcgga cacgatcgcc aaaagacgat
      481 cacttacaca ccctgggcgg agccggggct gcacgattcg accgggatgg cccgggcgag
      541 aaaatcaggc tttcttgggg cgcttcgcac catacttgga gcgcgcctgc ttgcgatcct
      601 tcacgccttg caggtcgagc gagccgcgca cgatgtggta gcgcacaccc ggcaagtcct
      661 tgacacggcc gccgcgcacc agaaccaccg agtgctcctg caggttgtgc ccttcgccgc
      721 cgatatacga gatgacctcg aaaccgttgg tcaggcgaac cttggcgacc ttgcgcagag
      781 ccgagttcgg cttctttggc gtggtggtgt agacacgggt gcaaacgcca cggcgctgcg
      841 ggcagttctc gagggcgggg cttttgctgc ttgcgcggct gacttcgcgc ggcttgcgca
      901 cgagttgact aatggtaggc atgaccttta cgcgtccttt tttacgtact cacgtgagtg
      961 gcgtaccaat tcacaatctg ctttggaaac gcctgcttcg gtgggccgcc cacatagggc
     1021 caacctcaaa cgaattcagg cggtttacgc aaaaaccgcc tgacacgatg aacgtaaaag
     1081 agcgggtctt gctggccgca ccgaccaacc aaccaaaggc gggcgtacaa aagtcggggg
     1141 catactgctt ggataagctc gccatcatat cattcgcgtt tccataaggt caagcaaaat
     1201 agcggctagc atccgcccag gcgcacaaca gcggccgtcc cagaggaccc ttggtggtcc
     1261 ggaatgatag gtacccttta aaatggcaat attcagaagc aaaaagaacc atagaccatg
     1321 caccttgccc tgctggaaga cgacccggac caggcgcgac tgatcgggca catgctcgcc
     1381 gaagccggcc acgaatgcgc cagcttcgcc agtggccgcg aattgctggc cgccctgcag
     1441 aaaagcgaac cgttcgacct cctgttgctc gactgggaag tccccgacct gaccggcctg
     1501 gaaatcgtcc gctgggtgcg ggcgcacatg ggttacacca ttcccgtcat tttcgtgacc
     1561 aaccgcatcc aggaggccga tctggtcgaa ggcctgcggg cgggcgccga cgactacatg
     1621 accaaaccca tcagccgcgt cgagctggcg gcgcgcgtgg cggcgctgtt gcgacgcggc
     1681 cacccccagg cgatggcaga cacggcgttc gaattcggcc cgtacaccat cgatccggcc
     1741 gagggcgtca ttctcctgaa cggcgaaaaa gtgccactcg cccccaagga attcgatctg
     1801 gcgctgctgt tcttccgcaa tcccgggcgc ctgttctcac gcgatgtcct cagcaccacc
     1861 gtctggaacc gcgacatccc ggccacctcg cgcacgctgg acacccacct gtctaacatg
     1921 cgccagaaac tggcgctgcg ccccgagcac gggtttcgcc tgacctcatc ctacgccctg
     1981 ggctaccgcc tcgaggccgt gaacgacaac atttgaacca cggaaccgtt cccatgccgc
     2041 ttctccgact tctgtccgcg ctggccctgg ctctgctggg tgccatgccg gcggtcgcgt
     2101 ccgcccaggc ggccggtgcc cagggcaccg actttctcta caacgccgtg tccggcgaca
     2161 cgctcatcgg cctgtcctcc cgctacaccc tgtccccgga tcactggcgg acgctgcgac
     2221 agatcaacca cgtggccgac cccgccaagc tctccatagg caaactgatc cacattccct
     2281 tcgcgctcat tcctgaagtc gcctcgacgg ccaacgtcac gcacgtcagc ggactggccc
     2341 agatcgatgg ccggccactg gcggttggcc agaaactggg ggaaggcagc atcgtgacaa
     2401 ccggtgccga tggtttcgcc acactgcaac tgaccgacgc cagcgtcctg acggttcccg
     2461 cgtcgtccac actggccatc cggcgcctgc gcagcttcaa gggcacgggg ctggtcgacg
     2521 ccatcatggc gttgaaacac ggcgggatcg aatccagtgt ggctccccgg cataccggcg
     2581 tgggacgctt cgagatccgc acgccactga gcatcaccgg cgtgcgcggc acacacctgc
     2641 gggtgcgcaa caacgcccgg ggcgcgcaaa gcgaagtggt gcgggggcgg gcgctcgtcg
     2701 cgtcgtcgcg gcaggacaag acggccacgc tgcatgccga tcagggtact ggtgtggccc
     2761 ccgacggcca catcctggcc atccgcaagc tgctgccggc gcccgccttg cccaaacccg
     2821 aacgcggcgg tgccgcgggc tggcgcatgg cgtttccggc ggtgccgggc gcatcggcgt
     2881 acctggtccg tgtcgcgacg gacgccgccg tcacccgcct ggtgtccacg cacacgttca
     2941 gcaagcccga cgtgaccttc aacgcgcccg gcgcgggaac cttctacgtc gcggtgcgcg
     3001 ccgtggaccc ggacggcatc gaaggcaacg acgccgtgcg cagcttcccg ggcgcccccg
     3061 tgctgaccag ctccgacggc ttgccggtgt tgtccggatc cgggcgcccc atcctgctgt
     3121 ccaggtactg agccgaccac catgctgacg gcgggcggcg gggcgcgcac cccattctgg
     3181 ggcaacctcg accggcgcat ccgactggaa tgggtgctgg tcctgatggc gctgtcgctg
     3241 ttcacagcgg cactcagttt cttcagccac gaggtcgggc tgacacgcct ggaccatacg
     3301 ttctacgaca acatgctggc cacggccacc agtcagccgg ctcgcgacga catcgccatc
     3361 gtggccatcg acgacgccag catcgcgcaa ctcggatact ggccgtggcg ccgccgcgtg
     3421 cacgcagacc tgctgcgcca cctgcgtggc gccaaggcca ttggcatgga catcgtcttc
     3481 agcgatccca acccggccta tccgggcgac gacacgctgc tggcgcaggc catttccgcc
     3541 gatggtaacg tggtgctccc gatgctcatc gacggcggcc gtgccctgac gcccatcgaa
     3601 ccgctggcct cggccgccaa ggccctgggc tacatcaatg tcaagcccga caacgacggg
     3661 gtggtccggt ccatgcgccc gcggcagaca ctggcttccg gcaagaccgt gacgcacttc
     3721 gccctggcct tgctgcgggt cgcaggcgcc acgccccgca taccgtcgtg ggcgggctcg
     3781 tcgaatctgc tcatccccta cgcggggccg cccggacact tcaccctcta tccgtatgcc
     3841 gccgtgctgg atggccgggt gccggcctcg gcgttcaagg gcaagtatgt cattgtgggc
     3901 gcctggggaa cggggctggg cgacacgttc cccacgcccc tgacccttca gggcaaaccc
     3961 atggcggggg tcgaaatcct ggccaatgca ctgcaggccg cgctgaccgg cgactggatc
     4021 cgcacgcccc cgcgcgggct ggccgccctg ctgtcctcgc tccctgtgct gctgttgtgc
     4081 ctgttcctgc gtcatctgtc gcctcggcgg ggctttttcg tcgccgtgct ggcgctgctg
     4141 ctgatctttc ttgcggacgc cctgttgctg cacttcgcac gggtctgggt ggcaccggcc
     4201 gccagcctga tcggcgtcat cctgaccgga ccggtctgga gctggcgcag ccaggaggcc
     4261 gcgctgcaac acatagaccg ggagctcgaa accttgcacg ccgaggactc tcgcatcggc
     4321 gcgcgccaac cctgtggccc gctccatgat ggctcactgc cggcccgcat caccttgctt
     4381 cacggcgcca taggccagat ccgccggctt cgcaccagcg aactcgcggc ccagcgccag
     4441 cgcgaggaaa ccctgcggtt catttcacac gacatgcgcg cgccgcagaa ttccattctg
     4501 gccctggcca gcctgcaatc agagcccgat acccggctgc cggaggagca actcctgcgg
     4561 cgcatcaacc agcacgcggg caggacgctg gccctggtcg acgggttcat ccagctggcg
     4621 cgggccgagt ccatggcgtt gcagacccgg ctcattgacc tggccgatct gatcgcgcag
     4681 ctgcgcgacg actattgggc tcttgccaag aagcacgaca tcacccttga ttgcgggcac
     4741 gacgtgtcgc ccggcggggc aatcgtcgac ggcgacgagt caatgatggc ccgggcgctg
     4801 ggcaatctcg tcgacaacgc catcaagtac agcgccgcca gcaccaccgt ggcatgccga
     4861 ctgtcgcgtc agggtgcgtc ctggcgcatc acaatcgccg atcaggggcg cggcatcgcc
     4921 cccgacaagc tgccggaact cttcacacca ttccggcgca tcgacgaaaa caccaccggc
     4981 aacccagccg gcagcggcct gggcctggcc ttcgtcaaga ccgtggtgac ccgccacggc
     5041 ggcaccattt ccgccgccag cgaaccgggg cggggagcaa cgttcacagt gctgctgccg
     5101 gccgttgacg ccgacgctga cgctgacgat aggcatggtg cctgagggac cgcaccagac
     5161 gcagaagcga ccgatccact cccatcccac acatgccacc accgcgagct gtctatcggg
     5221 gccaggggtg gggccggcgt aaggcgtgcg ccggagctgg ctcgggaagg cgggggcagt
     5281 cgtcgggcgc tgtctgagta aaacgggcag tccgtggggc tgaccgtttt gcgagtttcg
     5341 cccgacgccc gcctagacga gacagatccg gatgaagcac gccgtgccgg actcgcccct
     5401 ggccccgata gacagcgcct taaaatacca accgcagtct cgacgcgatc cagtgcaata
     5461 ccgcactgaa gtaaaaaccg ctgcacaagg cagcggaaac aagaagactc cattcacgtc
     5521 aggcgatact gaccactgct ctgcgcttcc gcagataacg cagcagcgga ggcaggacca
     5581 cagtggcggc cgagacgatc cacaacccct tggcgatgtt gctgtcaaaa agaacatgca
     5641 gctcaccatt ggtcatcgac aacgcgcgcc gcagattctg ttccatcatg tcgcccagca
     5701 ccacgccaag caccatgggc gccatcggta tgccggtctt gcgcaacaga tagcctatac
     5761 cccccaggat cgcagccatg gccagatcga aggttccggc gttgatggcg taaacaccga
     5821 tatagctgat cgccagaatg cccggcacca tgagccaacc cgggacctgc aacaccttgg
     5881 agaacacccg gaccatcggc acgttcatca ggaacagcat gatgttcgcg atgaacagcg
     5941 atgccaccag cccccacacg agctgcggct gctggtcgaa aagtactggc cccggcgtca
     6001 ggttgtagag cgtgagcgcc cccatcatga ccgccgtggt gcccgaacct ggaacaccca
     6061 gcgtcagcat gggcacgaag gcccccaccg ccgacgctgt ggcggctgct tcgggtgcgg
     6121 ccacaccgcg cagatccccc ttgccgaact tggcgttcgg gtcccggctc tcgcacaggc
     6181 gcttctcatt ggaataggcc acggcggatg ccacgcttgc ccccgcgccc ggcaatacgc
     6241 ccacgaagaa acccagcacg gcgctgcgca cgaccgtcca ccaggtgaac atccattcgc
     6301 tcacgttgaa caacttgcga ccgctggaat cgacctcgac cttgtgaccc gccgtgacgc
     6361 gttcgagcat ttccagcagt tccgccaccg cgaacagcgc gatgaccacc accacgaact
     6421 gtatgccatc gaacagatgg accgagccga acgtgtagcg atacacgccg gaattggcgt
     6481 ccaccccgac gatggcgatg gccaggccca gcaacgccga cagcgcgccc ttgaccggct
     6541 cgctgccgag caggctgctc aggcagcaga aggaaaagac catgagcacg aagtattcgg
     6601 ccggaccgaa ggccagggcc cagcccgcga gaagcggcgc aagcagcgtg atgccgatga
     6661 cggataccaa cgaaccaaaa aacgccgacc acgccgatag cgacaacgcc acgttggcca
     6721 gccccttgcg cgcgagcgga tagccatcga gtgtggtcat gatggcgctg gcttcacccg
     6781 gcacattgat gaggatggcc gtgatgcgcc caccgtattc cgcccctaca tacacagccg
     6841 ccagcaggat cagcgccgtt tcgggtggca ggttcatggc gaaggcgatg gggatcagca
     6901 tcgccacgcc attgatgggt cccagtccgg gcaacacgcc gaccatggtg ccgaaaaaag
     6961 aacccagcgc cgccaccaat agattggacc acgacgacgc cacatcgaaa ccgatactca
     7021 ggtgcgcgag aatctcgctc atgacacacg ctccagcaag ccgagcggca atacgacatc
     7081 gagcgcccga tcaaacagga aataaaagga cacgctcatg atcactccgc cggcggtgac
     7141 cttggcccaa cctccgccga acaggcggcc caccaggatg gtgaccagcg tcgtggcgat
     7201 gatgaagccc aggaggtcaa acaacaaggc ataggcgatc gcgtaaacca ccatgagcgc
     7261 gatgcgggaa ttggcgcccg gcggattgcg ctccgcggtg tgacggccgc ggaacgcgag
     7321 ccacaggccg caaagcccga tgctcagcgc cagcaacatg ggaaaggcgc gcggtccgac
     7381 cggctcatac gagaacgggg cctcgatggt tcggccaaac cacgccatga gtaccgcgaa
     7441 cgcgatcgtg gtgataccca ggatacggtc ggtcatagcg ttacttgcgg aggaggccaa
     7501 aggaatcggc gagctcggcg taactcttga cgcgctgcct cacgtacgcg tcgagctcgt
     7561 taccggtcat gcggaagggg aacagaccca gtttgtcgcg caatgcgtcg aacttgggat
     7621 ctttctgcat gtcgctgaac gcccggaccc accagtcgta ctcggcatcg gtcactttgg
     7681 ggccaacgta gaaaccacgg atgatgggcc actcaatgtc gaagccctgc tctttcgcgg
     7741 tggggatgcc ggacatctcg cccggcagac gcttgtcggc gaatacggcc agcacccgaa
     7801 tgggagagcc gcccttcatc atggtgaagg cttccgccgc gtcacccata taggcctgga
     7861 tgtgcccacc gcgcaacgct gtgttggcct caccgccccc ttcgaacgcc acgaagcgca
     7921 tcttcttgta gtccaccccc gcggccttgg ctgtgagggc ggccttcatc cagtcctggc
     7981 tgcccaccgt gccacccgcg cccagcacga tcttgctggg atcggctttc agcgctgtca
     8041 tcaggtcttg cagcgtcttg taaggggagt ccttgcgcac aatggccaca ccgaagtcgg
     8101 acccgacggc ggcaagccac cgcaccgcgt tggcgtcgta cttgccgaat ttcttctgtg
     8161 ccagattcag cagcgagccc ccggaaaagg ccacgatctt gttgccggcg gcggggtcct
     8221 gggcgacgat gtggttgtag gccaccgcgc cgatgccgcc cggcatgtag atgatgcgca
     8281 tgggttgctt caactggcca gtggccttca acccttcctg ggccaggcga caggtgaggt
     8341 caaagccacc gcccggcttg gccggcgcga tgcattccgg ccgctgaggc tcttgcgcgg
     8401 cggccgcgcc attcaaggcg aataccatcg tgcccagcag catgccggtt gcaagcgtac
     8461 gcatgaatgc gtttttcatt ggggtctccg tgtaggcgaa atgagtgtct gaatatgcgc
     8521 cgccccttcc ctggcgacga ctgatgcggc ggatgacgat ttcgatttct ttgtctattc
     8581 aacaaacccg ccattcgcaa aaacacgaca agcctacaga ccggttcctt tccaaaggct
     8641 ttcaattccc gcccgttcag gccggttcgc cgctcttctg tccggaaaca agggtaaaca
     8701 ctagtgttgc acgacacccc cggccctcgg actcgagacg gaattcgccg cgcagttgct
     8761 gcacgatggc atgcacgatc gccagcccca ggcccgcgcc cgatgtgttc ttgccggcct
     8821 tgccccgtcg aaaccgcacc cccgccttgg cgatgtcttc cacgctcatg cctggcccgt
     8881 cgtcatcgac ctgaatcaat gcgcgccccg cttcggaacg gacacgcaac gtcaccacac
     8941 cgcccgtcgg cgtgtagcgc aaggcgttgt ccagcacgtt gacaaccgct tcacgcaaca
     9001 accattccgg cccgcgcacg agtaccgcct cgtccggccg ctccagcccc agatcgaggc
     9061 ggcgggtgcg cgcggtcggg tacatgtcgc tggccacggc ggcggccagt ttcgccagat
     9121 cggtgaactc cgccccaccc tcctccagtg cctgaaagtc acggacgcgc gccaacgcca
     9181 gcatctggtt cgcggtgcgc accgatcgat ccagcccttc gcgcatcgcc cggaggacgg
     9241 cgcgaagctc ctgtgggtcc gattcccgca aggcatagtc cagttgcgcg cgcaacactg
     9301 tgagcggcgt gcgcaattga tgcgaggcgt catccaggaa ctggttctgc aggcttgcct
     9361 gccgcgcgta gcggtccata tgcaggttga tggcggtcac cagcggtgcc acctcggccg
     9421 gcagaccgcg cgcctccatc ggcctcaagt cgtcgatgcc tcgccccgcg acctcctggc
     9481 gcaaccgcgt cagcggcctg accgcgaaca gcaccgcgag cacaacgatc aggatcacca
     9541 acagcacggc ggccagatca cgttggaccg accgccacaa catggaattg atgaacgcct
     9601 cgcgcgtgtc cacgtcttcg ccgaccaaga cgacgacacg cccgctcgcg tgtccataga
     9661 gtggcggatc catgcgacgc gccatggcgg cgatgcgcac atccacgccc agataccggg
     9721 tggtgtagaa caccggaacg ttggatatca atgtgccgtc cggcaacggc aggcgctgat
     9781 tgccgacatc ggccagaccg tcttcggtca tcacgcggaa atagacgcgc tcgttggcgg
     9841 ctgtttcaaa aaattgaagc atcaagtacg gcaattccac tgacagccca ccgctggccg
     9901 tggagatctg gtgatcgatc gcgcgcaacg cccctgtcag cgagcggtcg taggcgaagt
     9961 tgatctgcgc gcgcagacgt tggttcgaca ggaacagcgc caccgtcagg aagagcacca
    10021 gtgcgggcac cagtgaccag agtaatatgg acttcagccc gcggctgcgc ccgaattcag
    10081 ttgtatccat acaccacacc aagattcccc atgcgcatac ttctggtcga agacgaaagc
    10141 gaactggcac gctggctcac ccgcagcctg gcccggcacg gcggcttcat cgccgactgg
    10201 gccgacgacg ccgaactggc cgagcgccgt atggccgtcg aggaattcga cgccgtggtg
    10261 ctcgatctgg gcctgccggg gatggacggc cagactttcc ttacccggtt gcgggctcgc
    10321 gatgaccgca cgccagtatt ggtgctgacc gcgcgcgatt ccctcgccga acgcgttcac
    10381 acgctgcatc acggcgccga tgatttcctg ctcaagccgt tcgtcatcga agagctggaa
    10441 gcacggctgg tcgcgctgat acgccgcagc cgcggccgtc accacccccg cctgtccctt
    10501 ggcgatctgg cgctcgacac cgcggcgcaa cgcttcacct ttggtggcca gccgctacct
    10561 ctctctccgc gcgaattcgc cgccttgaaa gtgttgatcc aaaaaagcgg cgaacccgtg
    10621 tccaaagcgc agatcctgga tcgcatcaat tccgacgacc acgacatcaa ccccgaggcg
    10681 gtggaggtgc tggtgcaccg cctgcgcaaa aagctggccg gcaccccggt gcggatcgcc
    10741 acgttgcgcg gaatgggcta cagccttgaa ctggatccgg gcgcggacgc gcccacgacc
    10801 ggatcctagt actgagagtc cagacatggc aaagccctgg ctgtgaggcc agggctttgc
    10861 cgggccgggc ctcgagaggc ccggcatacc gttggtgaaa ccccgaacgg cgtcaggcct
    10921 cggtgggacc gttgtccacg tggggctcgc tgtggtccgg agccggcacc acttccgtgg
    10981 tttcctccgt cgccgcggtg acaggcattt cctcgaacgg gttggccatg gcccgggcgg
    11041 tctggcgttc ggcggcctcg tgggcttcct tgtccttgcg ggcgatgtga tacgacatgc
    11101 ccgtgccggc ggggatcaaa cgaccgacga tcacgttttc cttcaggcca cgcaagtcgt
    11161 cgcgcttgcc catgatggca gcctcggtca gcacccgggt ggtttcctgg aaggacgccg
    11221 ccgagatgaa cgagtcggtc gacaacgacg ccttggtgat acccagcaac acgttttcat
    11281 atgtggcggg gatcttgccg tcggcttcga cgcgatcgtt ctcgttgagc aactcggagc
    11341 gttccacctg ttcacccgtg atgaagctgg tgtcgcccgc atcggcgatg ttcacgcgcc
    11401 gcaacatctg gcgcacgatc acttcgatgt gcttgtcgtt gatcttcacc ccttgcaggc
    11461 gatagacgtc ctgcacctcg ttcaccacgt aggtggccag gcgctcgata ccctgcagac
    11521 gcaggatgtc gtgcgggtcg gccggtccgt cgacgatcat ttcgcccttg ttcaccacct
    11581 ggccgtcgtg caccagaacc tgcttttcct tgggaatcag gaactcgtgg ttcacgcctt
    11641 ccaggtcggt gatgaccagg cgctgcttgc ccttggtgtc cttgccgaac gaaaccgtgc
    11701 cggtgatgtc ggccagcatg ccggcatcct tgggcgaacg cgc
//
LOCUS       DASTNK010000098        17303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1550213, whole genome shotgun sequence.
ACCESSION   DASTNK010000098 DASTNK010000000
VERSION     DASTNK010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 17303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17303
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550213"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VFR20_07515"
     CDS             complement(<1..461)
                     /locus_tag="VFR20_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HEU0230403.1"
                     /translation="MVFRWPLLAALVVVVLTGCATTPRTPAPDVRLVPWLSASGKYGY
                     VDESGALRIPAQYDDARPFYHSLAAASQDKRWGYIDTLGRWVVGPHYDKAYDFNANGQ
                     GEVLTVSDAFNLGGSLLPMYLTGKRVYYRVYDADGLLVKQGKINGAGHISD"
     gene            complement(739..1953)
                     /locus_tag="VFR20_07520"
     CDS             complement(739..1953)
                     /locus_tag="VFR20_07520"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HEU0230404.1"
                     /translation="MAHDNAISVPAIAPAASVQPPMRYMFKAWALAGAAAVAVGFARF
                     DYGLILPAMKADLGLTYAQAGWLNTVNGLGYLLGALLAIFGVGRLGTRRLFAWGTALT
                     AAALLGTGLTHGIGWLAAWRFLAGLGGAGAFICGGVLAGALGARAITIFFGCGIGFGV
                     VAGGAILPWLFDAYGPTVWPQAWLLTGAVCVLLCALAIRAMRGIAEPSSRHLDAGGSW
                     RGCAAVYMAYLVFGLGYIAYMTFMIAWVRQHAGQHGQLTLLVSAMWVVLGLACFLAPV
                     LWRGVLERCRNGRVMSAALLVLVLGAALPLAFGGVTGIMLSALLVGVSVVIVPSSVTH
                     FVKANLPKTAWGSAMAVATSLFAAGQVIGPVACGWLSDHYGSLSVGLAASAVVLLLAA
                     VLAAWQQALNRA"
     gene            complement(1943..2569)
                     /locus_tag="VFR20_07525"
     CDS             complement(1943..2569)
                     /locus_tag="VFR20_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0230405.1"
                     /translation="MPRPSASQPPARDRIVACASDLFYRKGINHVGINEIIDASGIAR
                     MTLYHHFASKNDVVVAVLEQRMAQRRQAMQAAIDSAQSPRDKVVAAFAYLREIVDAQD
                     FRGCVFINATVELADPGHPGAILSARHKRRMADCFTRIAQDAGWPDADSFGIQCQLLW
                     DGAAAAAQVNYTDAPVDAACQAIEVLIAAREAAAKQERGNAAGGHHGA"
     gene            complement(2734..5616)
                     /locus_tag="VFR20_07530"
     CDS             complement(2734..5616)
                     /locus_tag="VFR20_07530"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HEU0230406.1"
                     /translation="MGKATFAYSGKAFPDSRESVVPNACIHALVEGRAAVRLPGAMDD
                     AGGKGDKSDKKTRGPGSNAKWGFLDRDGHLAIKPVFDAVSDFSHGLAAARKNGLWGYI
                     DAQGKWVVAPAFTAAGPFSSSGLASIEKDGRFALIDRQGKVTGAVFGREVLDVAIGDG
                     TPSLVGLRYRDRWLSPKGAIRYAPPGATLKQSLGSQGLFIAADASGNLGVVDEQGHWR
                     VPARYRSMEESYLPHGLVHARGTGRGNDVLINERGGIVAGKGFRRIQALGKHFWLLEK
                     DGRKAVLADAQGRVIQDLGAHRVIAIGPFAMVSLGDTTSQVFVPGQAAPLTISGSLMP
                     DSGLRDGVLLWRDAGGQSWRYGGGAVAGILTRNGHWLTGLPWLASIQSTFPVGPYLVF
                     RDRHDALLNIVNAEGKPMLSAETVKELDQVGVTFLPAASDASTQPVAIAILSACHCGT
                     PKGAGLLLSDGTLLRNAAWQSIRSLDGEKDGEALNADDSPRSRQSPGGQRFVVQTATG
                     LGMVDAAGHLLIPATQSHIGEFSDGLSLVFGNGRSYIANRTGSLAPAPNYFLVEAAGP
                     GLLRFHDTAAGDAPWGLYDLKSGHVLVPPTLRALGDFRNGYAVARDARGNAGVLDLHG
                     RWVVPARFKSLKPLGPRTWEANGSAYKSSIVTLDGKELVPPVPKLYTQVLPGGYVEAS
                     TNGNDGSGAAAWLMDGTGKVVAGGPGRHIRDMGNGWWRIHRDLQQAYVAADGQWVMAP
                     SSVEGSPFHAPSGLALIQRGLDAHLIDAQGKAVVALPKGVWKWTAGSPWLRTFQPGLD
                     GAPGRTDYADVHGKIVLSVQGLTGPFDDGYVLARLQDGRGFSWVDDKGKVVPMRPYQD
                     LGLPSDGLAFASAKGLYGYVDRQNRYVIAPAFQAASAFNQGRAVVSTRHASMIIDKMG
                     RPLARVTTRCGVRVLLDADNRMVWPAALPTDCGMRHGTASALTPER"
     gene            5864..7915
                     /locus_tag="VFR20_07535"
     CDS             5864..7915
                     /locus_tag="VFR20_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230407.1"
                     /translation="MPGFLAVLTVIAASFHVPTAHAATPPTPQSVDPALFHQGLERAS
                     IAYKRHDYATALQYLYQAWRNTWRIDDRERVGNGVAFIQERQHNYAGAQATLSGILPL
                     TLCPPPSQDDTRQAYVRIARLAWLAGEPRQTYALLRQAYGTHPVRSWGWGGLDRDHWH
                     LSSDRAGLTYDLAGMTFPATTAGFLRGTYASADDSHDMASVDYVPLRADNPFASGKVT
                     IAISTRQGHAETVAHDLRQWLAIAAGIPLAKEKADLEIRPTQAPTIQPVTEPRGKQPA
                     EAAHTASGTIEAADSDGVAHEYGMWISRQGVWQIEVHADWPAKNRDAAQHAIAGLMAA
                     ILWKPGQAAYHGTGDAALELDKQFDLAMLRKQWPTAAELARKRTGSAVFPHRRARLYS
                     AIAIAAMFERHYPDAMNAMHKAMHYWHYTSLDHDDEGFYDTLLLDAANIAFHLGDNAQ
                     GITWLTAREQDQLDPKWTMDKTSGSLRHNPTGVVLPARLGSFLRIWHEGNKARYQRIQ
                     PSQYLGITLLDKIPGHSNGATPSEAHGKPPDAYPDAGTNPSNANDVQAIETSLRTWMT
                     RGLGLTVGAMRQAAYPPAGVGHAHGIQFLFDVTRTDNDGTPHQRTMGFWVSTLQGRAI
                     VLRSGWARGDARGEQAAATAAAAFAWPQTLRAVAGKQRFLDLQCKAPAMAKAFLPGMQ
                     P"
     gene            complement(7895..10636)
                     /locus_tag="VFR20_07540"
     CDS             complement(7895..10636)
                     /locus_tag="VFR20_07540"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HEU0230408.1"
                     /translation="MPTQMCDDSIRPELCFQSFHDGLAALQLGADHGGRVLWGYIDKS
                     GQLSIAPRFTDAQPFSHGLAAAEDGKHWGYIDKQGNWVIPATYDSATAFGDQGVALVA
                     QRERELLIDRTGTVIKQLAPGTREEYDGFGRDNGVAVLQVPVQPLAWRVRDGKSLALP
                     TDVMEVAPQQGKWIPAMQRTGRFTGVWGYLDASGKWVVPPSVLQATRVPMTDGSLIAV
                     AHGNEMALVDATGKAVANHAFLIRLLAPGVWLVKDKTTARLLADQGKALHDLGDRNAV
                     EVDRDLRGTDLAALVSSRTGIYFIRRDGSMRELADSRALVRVQGHYLFVTRKDAQGND
                     ALTQVLDGNGKPLLDPSTVKQLSGYSAYALLGDHPVPAGKAAFIALLSPADYTKPSAL
                     LTSDGRLLMGTAWRTADTGQSMGPLAVVKGKDDKYGVIDLKGNWVVKPVYAFIGGYRD
                     GYALARKARGFNAAVVMLDTQGKEHTIAPHVEQNIVKRGDEVFVYRQRGGDTPDKYGL
                     WRIDQGRIVRKPDLDEIHDFDHGHALARKGKEWGDLDTAGNWQPIAGLKDAYHARRQG
                     DVYVVETRGAQDESGERQSSFDVYSIITRGWVVRDLRKRPTGVGKGRYLVQPVSGGAE
                     LVDGRGKVLARTDRDVSDVTSGQGWVVLRSDNRYGAIDAKGNWQVPPVYDFHFSFVKP
                     SMLASVYKSGRYELIDTHGKRVASPVAWAKPVPGMERLVYTDGTKAASAMTTLSGDEV
                     ARLPGSYAFDVDQAASGFIPFEDHATGLRGFMDAGGKKVIGAYFSKLDGMVDDRAGAQ
                     MKDRYGKYYGFIDRSGRFVIPPRYDWVMPFSEGRAMVSRNQVWSYIDKAGNPVARLAV
                     RCGKVVLEDGTGAQRWPKAVDTCVPPSAVQALSGPLGNQAAPGSPPPPPPPSRLHAR"
     gene            complement(10954..11562)
                     /locus_tag="VFR20_07545"
     CDS             complement(10954..11562)
                     /locus_tag="VFR20_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230409.1"
                     /translation="MFFVSPPTAGAAAITDSSAPKQLPNGDVVSSITTEAGKTYTLTY
                     RQAAQDIQVQHAASTAVVSRIPRGHDPSLVGAATRIRFLPNSLQPYESRGDLLFIATQ
                     RTRAGRGMGLCGAGVETYLDVLDIRHKSPKVTASLLVDSCARGISLRNNGDPDDTLYS
                     FDVEGGRLSMRFLAYEDMTRSFGIRAVLSDDLKRLLVMHIPR"
     gene            complement(11741..12589)
                     /gene="kdsA"
                     /locus_tag="VFR20_07550"
     CDS             complement(11741..12589)
                     /gene="kdsA"
                     /locus_tag="VFR20_07550"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEU0230410.1"
                     /translation="MKSVEITHGIVCANDRPFVLFGGINVLESEDLALRACDEYVRVT
                     NKLGIPYVFKASYDKANRSSIRSYRGPGLDEGLRILDKVKAEHGVPVLTDVHEPGQAA
                     AVAQVADVLQLPAFLARQTDLVTALARTGKPINAKKPQFISPGQMLNIVEKFHEAGND
                     RILLCERGACFGYDNLVVDMLGFGVMKKACGGLPVVFDVTHALQQRDPLGLASGGRRS
                     QVSELARAGVAVGLGGVFLEAHPDPDHAKCDGPSALPLDKLEPFLRQLKALDDLVKSL
                     DAIDIE"
     gene            complement(12731..13555)
                     /locus_tag="VFR20_07555"
     CDS             complement(12731..13555)
                     /locus_tag="VFR20_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0230411.1"
                     /translation="MFTTHAAPLPVVVPRAIDLIEDTDRDVIVVETAHSTGYVIPAHT
                     HKRVQLLHATTGIMRLETKYGAWIVPPGYAVWIPADVVHRVSTINVTTRSLYVREGAL
                     AQPPEKCRVIQVSPLLRELIAAAMKVPLLYECDARDGRLMDMVLCEAGAQPEVSLHLP
                     MPSEPHLAALCHVFFESPTQASAPADWAARLHVSERTFYRRFLANTGMTFINWRQQAC
                     VLVAMSRLSLGDSVTRIALDMGYENPSSFSTMFRRAMGVAPTVYSRNAGRGAATEA"
     gene            13685..14905
                     /locus_tag="VFR20_07560"
     CDS             13685..14905
                     /locus_tag="VFR20_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0230412.1"
                     /translation="MNNAAATTLKPATGTGISYGVLGAISTTHMINDMMQSVLLALYP
                     VLQGNFKLSFVQIGFITVAFQFSASLLQPIIGRFTDKRPQPYSLPIGMTFTMVGLLLL
                     SRAWNYPCVLAAAVLIGMGSSVFHPESSRVARMASAGRHGLAQSIFQVGGTLGSSIGP
                     LLAALLIVPLGQPSVAWVALAALVGIIILLQVSRWYSHNLTSVRGRSSLSRTDTGLTS
                     KQVRSGLTVLLILVFSKFLYLSSLNSYFTFYLMHHFGLTIQHAQYCLFVFLFGAALGT
                     MVGGPVGDRIGRKRVIWGSILGSAPFALILPYVNLPATIVLIFFIGLILSSAFPAIVV
                     YGQELVPGKTGTISGLFFGLAFGIAGIGAAALGALADSLSIEFVYHVCAFLPLLGIVA
                     FLLPDMRGGKKAAA"
     gene            complement(14976..15680)
                     /gene="bioD"
                     /locus_tag="VFR20_07565"
     CDS             complement(14976..15680)
                     /gene="bioD"
                     /locus_tag="VFR20_07565"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEU0230413.1"
                     /translation="MKHAYFVAGTDTEIGKTLTASSLLYALTRTGVRTAGMKPVAAGA
                     ELRDGTWHNEDTDSLTAQASVALPTELVTPYLFRQPTAPHIAARREGRTIELPHILAC
                     YRRICELADAVVVEGVGGFRVPLNQGVDTADLAQKLNLPVVLVVGLRLGCINHTLLTV
                     EAIAARGLPLAGWVANTVDVGLLNSDATVDALASRIEAPLLGKVPRFPPDPRHGTELV
                     SAVARCLDFSLLPGWP"
     gene            complement(15687..17030)
                     /gene="bioA"
                     /locus_tag="VFR20_07570"
     CDS             complement(15687..17030)
                     /gene="bioA"
                     /locus_tag="VFR20_07570"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEU0230414.1"
                     /translation="MVRRSLRSVWHPCTQMQHHETVPLVPISHGRGAWLYGVDGKRYL
                     DAISSWWVNLFGHANPRINAAVAAQLEKLEHAMLAGFTHEPVIRLSECLAERTGNRLG
                     HCFYASDGASAVEIALKMSFHAWRNAGHTSKHEFVCLEGSYHGETIGALAVTDVALFR
                     DAYDPLLRRAHVVMSPDARAARNGETATDVARRAANALESLLRQRSSHIAAVIIEPLV
                     QCATGMAMHDPVYLSEVRALCDRYDVHLIFDEIAVGCGRTGTFFAAEQATAIGVDGLP
                     VWPDMVCLSKGISGGYLPLSLVMTTDDIYQAFYDTDVTRGFLHSHSYTGNPLACSAAL
                     ATLEIFEQDDVLNANRARARRITAGLQGVAHHPRVRDFRHRGMIWAFDVAIDDSAAAA
                     GFSRRFFTKALENELLLRPIGRTVYLMPPYVLDEMECEMLASRVTSVFEEVIAHA"
BASE COUNT         3502 a         5758 c         5011 g         3032 t
ORIGIN      
        1 tagtcggaaa tatgcccggc accattgatc ttgccctgtt tgacgagcag gccgtcggcg
       61 tcgtagacgc ggtaatagac gcgcttgccc gttaggtaca tgggcagcag ggaaccgccc
      121 aggttgaacg catccgaaac agtcaacacc tctccctggc cgttggcatt gaaatcgtac
      181 gccttgtcat aatgcgggcc gacgacccaa cgccccaggg tatcgatata gccccatcgt
      241 ttatcctggg acgcagcggc caggctgtga tagaacgggc gcgcgtcatc gtactgcgcg
      301 gggatgcgca gcgctcccga ctcatccacg tacccgtact tgccgctggc ggaaagccag
      361 ggaaccaacc ggacgtcggg cgcgggcgtg cggggcgtgg tcgcacatcc cgtcaaaacc
      421 accacgacca atgccgcaag caacggccac cggaaaacca tgccaagggg caatgatgcg
      481 caggggagtc tcatcttagt ggaaccatca tggaaacagt agatacactg tagactatta
      541 cacctgatga atccaccggt tgccctcaac attggttgcg caaacatgac atcgtctttg
      601 ggctgcatac gcgctaaaaa tggacactga caccttgtcc tgcacactca gggcttcgcc
      661 caccggaatg aggaaggcag cgagccgcgg atgcatgcgg cgcgatgact tgttcaatgg
      721 atcctcaggt attgcgtatt acgcgcgatt cagcgcctgc tgccacgcgg ccagcaccgc
      781 cgccaacagc aggacgaccg ccgaggccgc caggccgact gacagcgagc catagtggtc
      841 gctcagccac ccacaggcca ccgggccgat cacctggccg gcggcgaaca ggctggtggc
      901 gaccgccatg gcgctgcccc acgcggtctt cggtagattc gccttcacga agtgggtgac
      961 tgacgagggc acgatcacca cactgacgcc aaccaacagc gccgacaaca tgatgcccgt
     1021 cacgccgccg aaggccagtg gcaatgcggc ccccaggacc agcaccaaca gcgccgccga
     1081 catgacccgg ccgttccggc atctttccag cacgccgcgc cacagcacag gtgccagaaa
     1141 gcacgccaac cccagcacta cccacatcgc cgacaccagc agcgtcagtt gcccgtgctg
     1201 tcccgcatgc tgacgcaccc aggcgatcat gaacgtcatg taggcgatgt agcccagccc
     1261 gaacaccaga taggccatat agacggcggc gcacccgcgc cacgatccgc ccgcgtccag
     1321 atgccttgac gaaggctcgg cgatgccccg catcgcccga atggccagcg cgcaaagcag
     1381 cacgcacacc gctccggtca acagccaggc ctgcggccac acggtggggc catatgcgtc
     1441 aaagagccag ggaagaatgg cgccacccgc caccacgcca aagcctatgc cgcacccgaa
     1501 gaaaatggtg atggcgcgcg cgcccagcgc gccggccagc acgcccccgc aaatgaacgc
     1561 ccccgcacca cccagccctg cgaggaaacg ccacgcggcg agccagccga taccatgggt
     1621 cagacccgtg ccaagcaacg ccgcggcggt cagcgcggta ccccaggcaa aaagacgccg
     1681 ggtgcccagc ctgcccacgc caaagatggc caacagcgcg ccaagcaaat accccaggcc
     1741 attcacggtg ttcaaccagc ccgcctgcgc atacgtgagc ccaaggtccg ctttcatcgc
     1801 gggcaggatg aggccgtagt cgaagcgcgc gaaaccgacg gcgacggccg ccgcgcccgc
     1861 gagcgcccag gccttgaaca tatagcgcat ggggggttgc acactcgcgg ccggcgcgat
     1921 cgcgggcact gaaatcgcgt tgtcatgcgc catgatggcc ccctgcggcg ttgccgcgtt
     1981 cttgcttggc cgcggcttcc cgcgcggcga tcaatacttc gatggcctgg cacgccgcat
     2041 cgaccggcgc atcggtatag ttcacttggg cggccgccgc cgccccatcc cacagcagct
     2101 ggcattggat gccgaagctg tcagcatcgg gccacccggc atcttgcgcg atgcgtgtga
     2161 aacagtccgc catgcgccgc ttgtgccgcg ccgacaggat ggcgccgggg tggccggggt
     2221 cggcaagttc gacggtggca ttgatgaaca cgcagccacg gaagtcttgc gcatccacga
     2281 tctcacgcag ataggcgaac gcggcaacca ccttgtcgcg cggtgattgg gccgaatcga
     2341 tggccgcctg catggcctgg cgacgttgcg ccatgcgctg ctccagcacg gccaccacca
     2401 cgtcattctt ggacgcgaaa tggtgataga gcgtcatgcg ggcgatgccg ctggcatcga
     2461 tgatttcatt gatacccacg tgattgatgc cctttctata gaacagatcg gacgcgcaag
     2521 cgacgatgcg atcgcgcgcg ggtggctggg aagctgaagg acgaggcatg atgattgact
     2581 cacgggaaca ccggcacagt ttcagatagt cactgtgcag gtttatttat atagactggt
     2641 ctgtataata aacattcgtc attggtgctg tcaatgtctc agtcaaaccg tcaggaatct
     2701 ggatcccgcc agacccgtgg cctgcacgtc gccttagcgt tcgggtgtca gcgccgacgc
     2761 tgtgccgtga cgcatcccgc aatccgtcgg caacgccgca ggccagacca ttcgattgtc
     2821 ggcgtcgagc agaaccctga cgccgcagcg cgtcgtgacg cgcgccagcg gcctgcccat
     2881 cttgtcgatg atcatcgatg cgtggcgggt ggacacgacc gcccgaccct ggttgaacgc
     2941 cgatgcggcc tggaacgctg gcgcgatcac atatcgattc tggcggtcga catacccata
     3001 caaccccttc gcggaggcga acgcaagacc gtcggaaggc aagcccagat cctggtatgg
     3061 ccgcatcggg acaaccttgc ccttgtcatc cacccacgag aagccacgcc catcctgaag
     3121 ccgcgccagc acatacccat cgtcgaacgg cccggtcaga ccttgcacac tcaacacaat
     3181 cttgccatgg acatcggcgt aatccgtgcg gcccggcgca ccgtcgagtc ctggctggaa
     3241 cgtccgcaac cagggcgagc cggccgtcca tttccacacc cctttgggca gcgcgacgac
     3301 cgcttttcct tgcgcatcga tgaggtgtgc gtccagtcca cgttgaatca gggccagccc
     3361 cgaaggcgcg tggaatggcg agccctcgac actggatggc gccatgaccc attgaccgtc
     3421 ggcggcgacg taggcttgtt gaagatcacg gtggattcgc caccagccat tacccatgtc
     3481 gcgaatgtgc cgaccgggtc cgccggccac gactttcccg gtgccatcca tcagccacgc
     3541 cgcggcgccc gaaccgtcgt tgccattcgt gctggcctcg acatacccgc caggcaatac
     3601 ctgcgtatac agtttcggca ccggcggcac cagttccttg ccgtccaggg tgacgatgct
     3661 gctcttgtag gcggagccgt tggcctccca ggtgcgcggc cccaatggct tcagtgactt
     3721 aaaccgtgcg ggcacgaccc accggccgtg aagatccagc acaccggcgt tcccccgcgc
     3781 atcgcgcgca acggcatagc cgttcctgaa gtcaccgaga gcccgcaacg tcggtggcac
     3841 gaggacgtgg cctgacttca ggtcgtacag gccccatggc gcgtcgccgg cggcggtgtc
     3901 atgaaaacgc aggagtccgg ggccagccgc ttccaccaga aaataattgg gcgcgggcgc
     3961 cagagaaccc gtgcggttgg cgatatatga ccggccattg ccgaacacca gactcaagcc
     4021 gtcggagaac tcgccgatat ggctctgtgt ggccgggatg agcagatgac ccgcagcatc
     4081 caccatgcct agcccggtgg ccgtttgcac cacgaagcgc tggccacccg gcgattgacg
     4141 agagcgcggg gagtcatcag cattcaaggc ttcgccatcc ttctcgccat cgagactgcg
     4201 aatggactgc caggccgcgt tccgcaacaa ggtgccatcc gacaacagca gtccggcgcc
     4261 cttcggtgtg ccgcaatggc aggcgctcaa gatcgcgatg gcaaccggtt gggtcgaggc
     4321 atcggacgca gcgggaagaa acgtgacacc cacctgatcc agctccttca ccgtttccgc
     4381 tgacaacatg ggcttgccct cggcattcac gatgttcagc aaggcgtcat gccggtctcg
     4441 aaacaccagg taaggcccca cgggaaaagt cgactgtatg gatgcaagcc atggcaaccc
     4501 cgtgagccag tgaccgttgc gcgtgagaat acccgcgacc gcgccacccc cgtatcgcca
     4561 cgactgcccc cccgcatcac gccagagcaa tacaccatcg cgcagccccg aatcgggcat
     4621 caaactaccg gatatcgtca gaggcgcagc ctggccaggc acgaaaacct gtgatgtcgt
     4681 gtcgcccaga ctcaccatgg caaaggggcc gatagcgatg acgcgatggg cgccaaggtc
     4741 ttgaatgaca cggccctggg catccgccaa caccgccttg cggccgtctt tttccagcag
     4801 ccagaaatgc ttgcccagtg cctgtatgcg ccgaaacccc tttcccgcca cgatcccgcc
     4861 ccgctcattg atcagaacgt cgttgcctct gccggtgcct cttgcgtgaa caagaccgtg
     4921 cggcagatag gattcttcca tcgaacgata ccgcgccggc acccgccaat gtccctgctc
     4981 atccacgacc cccaggtttc cggacgcgtc ggcggcaatg aacagccctt gtgaacccag
     5041 tgactgtttc agggtggcgc cgggcggtgc gtaccggatc gcccccttgg gagacagcca
     5101 cctgtcgcgg tacctcaacc caaccaggct gggcgtgcca tcgccgattg ccacatccag
     5161 cacctcgcga ccgaaaaccg cgccagtgac cttgccctgt cgatcgatca gcgcaaagcg
     5221 cccatccttc tcgatgctgg ccaggccgct tgaggaaaaa ggccccgccg ccgtgaaggc
     5281 cggcgccacc acccatttcc cctgcgcgtc tatatagccc cacaacccat ttttcctggc
     5341 cgcggccagg ccatgcgaga aatccgagac agcgtcgaaa accggcttga tggccagatg
     5401 gccatcacga tcgagaaagc cccatttggc gttgcttccc ggcccacgcg ttttcttgtc
     5461 gctcttatcc cccttgcccc ccgcatcgtc catggcaccc ggcaaccgca ccgccgcccg
     5521 gccctccacc agggcatgga tacaggcgtt gggaacaacg gattcccggc tgtcgggaaa
     5581 cgccttgcct gaataagcga aagtcgcttt gcccatgcaa gaggttcctc tggcatccgc
     5641 gcggacaggc gtaccgctta tcacaatcaa gatataggtc acagccaaca ggctgtggat
     5701 ctttgcgcgg tacgtcatgt caatctgcaa gtttgaaaac acaaattgta gtggcgcccc
     5761 cccatcgcct ggctgtaagg ttgcacgtgg taacatcata tctacatttt tcatgaccac
     5821 catcaaaaaa aatcgcctcc gacagccgct caggacacga gccatgccgg gatttctggc
     5881 ggttctgacc gttatcgcgg cgagctttca tgtgccgacg gcgcacgcgg ccacgccgcc
     5941 caccccgcag tccgtcgacc ccgccttgtt ccaccagggc ttggaacgcg cgtcgatcgc
     6001 ctacaagcga cacgactacg ccacagcgct gcaatacctg taccaggcat ggcgcaatac
     6061 gtggcgcatt gacgatcgcg agcgggtcgg caacggcgtg gcgttcatcc aggagcgcca
     6121 gcacaattac gcgggcgccc aggccacgct gtctggaata ttgcccctga cgctctgccc
     6181 gcccccctca caagatgaca cgcgccaggc ttacgttcga atcgcccgac tggcctggct
     6241 ggcaggcgag cccaggcaga cctatgccct gcttcggcag gcgtatggca ctcaccctgt
     6301 ccgatcctgg ggttggggcg ggctggacag ggaccactgg cacttgtcga gcgacagggc
     6361 cggcctgacc tatgacttgg ctggcatgac tttccccgcc acgacagctg gcttcctgcg
     6421 cggaacatac gccagcgccg atgacagtca tgacatggca agcgtggact acgtgccgct
     6481 gcgtgccgac aaccctttcg ccagcggcaa ggtaaccatt gcgatcagca cacgccaggg
     6541 ccatgcggaa acggtggcgc acgatctgcg ccaatggctg gctatagccg ctggcatccc
     6601 gttggcgaaa gaaaaggctg atctcgaaat ccggcctacg caggccccca cgatacaacc
     6661 tgtgacggaa ccacggggaa aacaacccgc tgaagccgca cataccgcgt cgggcaccat
     6721 tgaagccgcg gatagcgacg gtgtggcgca cgaatacggc atgtggatat cgcggcaagg
     6781 cgtctggcag atagaagtcc atgccgattg gccggcaaag aaccgcgacg ccgcgcaaca
     6841 cgccatcgcc ggtttgatgg ccgcgattct ctggaagccg ggccaggccg cgtaccatgg
     6901 cacgggcgat gcggccttgg aattggacaa gcaattcgat ctggctatgc tgcgaaagca
     6961 atggccaacc gccgcggaac tggccagaaa acgcacgggt tcggcggtgt tcccgcatcg
     7021 ccgggccaga ctgtacagcg ccatcgccat cgcggccatg ttcgaacgac attatccaga
     7081 cgccatgaac gccatgcaca aggccatgca ttattggcat tacaccagcc tcgatcacga
     7141 cgatgaaggg ttctatgaca ccttgctgct cgatgccgcg aacattgcgt ttcaccttgg
     7201 ggacaatgcg caaggcatca cttggctgac cgcgcgcgag caagatcaac tggacccaaa
     7261 atggacaatg gacaagacgt ccgggtcgct gcgccacaac cccactggcg tcgtgctgcc
     7321 agcccgcctg ggaagtttcc tgaggatatg gcatgaaggc aacaaggcac gctatcagcg
     7381 gatccagcca tcgcagtatc tgggcatcac gttgctggac aagatcccgg gccactccaa
     7441 cggcgcaaca ccgtccgagg cccatggcaa accacccgat gcataccccg atgccggaac
     7501 caacccatca aatgccaacg atgtccaagc cattgaaacg tcgttgcgca catggatgac
     7561 ccgcggactg ggtctgacag tgggtgccat gcggcaggcc gcatacccac cggccggtgt
     7621 gggacacgcc cacggcatcc aatttctgtt cgatgtcacc cgcaccgaca acgacgggac
     7681 gccgcatcaa cgcacgatgg gattctgggt cagtaccctg caaggccggg caatcgtgct
     7741 gcgtagtggc tgggcacgcg gtgatgcgcg gggagaacag gccgccgcca cggccgccgc
     7801 ggccttcgcg tggccccaga ccctgcgcgc ggtcgccggc aaacagcgct ttctggacct
     7861 gcaatgcaaa gccccggcca tggcaaaagc cttcttacct ggcatgcagc cttgatggcg
     7921 gaggcggcgg aggtgggctg ccgggcgccg cctggttccc aagcggcccg gatagcgcct
     7981 ggacagcgga cggcggaacg caggtatcca ccgccttggg ccagcgctgc gcacccgtgc
     8041 cgtcttccag cacgaccttt ccgcaacgca ccgccaggcg cgcgacagga ttgccagcct
     8101 tgtctatgta cgaccacacc tgattgcgcg acaccatggc ccgcccctcg gagaacggca
     8161 tgacccagtc atagcgcggc ggaatgacga accgcccgct tcgatcgatg aatccataat
     8221 acttgccgta gcggtctttc atttgcgcac cggcgcgatc atcgaccatg ccgtccagct
     8281 tgctgaaata cgcgccgatg accttcttgc cccccgcgtc catgaaaccg cgcagccccg
     8341 tcgcatggtc ctcgaaggga atgaatccag aggcggcctg gtcgacatcg aacgcatagc
     8401 ttccgggtaa gcgcgcgacc tcatccccgg aaagtgtcgt catggcggac gccgcctttg
     8461 tgccatcggt gtacaccagg cgctccatgc caggcaccgg cttggcccat gccaccgggg
     8521 acgccacacg cttgccatgc gtatcgatga gctcgtaccg gcccgatttg tacaccgacg
     8581 ccagcatgga tggtttcaca aaggaaaaat ggaaatcgta gacggggggc acctgccagt
     8641 ttcccttggc gtcgatggcc ccataacgat tgtctgaacg cagcaccacc cagccctgcc
     8701 ccgaggtgac atcgctcaca tcacgatcgg tacgagccag gaccttgcca cgcccatcca
     8761 ccagttcggc gcctccggac actggctgca ccagataacg ccctttgccc accccggtgg
     8821 gacgcttgcg caaatcgcgg accacccagc ctcgcgtaat gatgctgtac acatcgaagg
     8881 acgattgacg ttcgcccgac tcgtcctgcg cgccgcgcgt ctccaccaca tagacatcgc
     8941 cctgcctgcg ggcatggtag gcgtccttca accccgctat aggctgccag tttcccgctg
     9001 tgtcgagatc cccccactcc ttgcccttcc gagccagcgc atgtccgtga tcgaaatcat
     9061 gtatctcgtc cagatcgggc tttcgcacga tgcgtccctg gtcgatgcgc cacaggccat
     9121 atttatcggg cgtgtcacca ccgcgttgcc tatagacgaa cacctcgtcg ccgcgcttga
     9181 cgatgttctg ttcgacgtgt ggcgcaatgg tgtgttcctt gccttgcgtg tcaagcatga
     9241 ccacggcggc attgaacccc cgcgccttcc gggccagcgc gtaaccatct cgataaccgc
     9301 ctatgaacgc gtacacaggc ttgaccaccc agttcccttt caggtcgatg acgccatatt
     9361 tgtcgtcctt gcccttcacg acagcgagcg gccccatgga ctgccccgtg tcggcggtgc
     9421 gccacgcggt gcccatcagc aaccggccat ccgacgtcag caaggccgag ggcttggtat
     9481 agtccgccgg gctcagcagc gcgatgaacg cggccttccc ggcgggcacc gggtgatcac
     9541 ccaacaaggc gtaggcgctg tacccggaaa gttgcttcac tgtcgaggga tcaagcaacg
     9601 gcttgccgtt cccgtccagg acctgggtca acgcgtcatt gccttgcgcg tccttgcgcg
     9661 tcacgaacag atagtgcccc tgcacacgaa ccagcgccct ggaatccgcc agttcgcgca
     9721 tcgaaccatc cctgcggatg aagtaaatgc ctgtcctgga tgacacaagt gcggcaaggt
     9781 cggtgccccg caagtcgcgg tcaacctcca cggcattgcg atcccccaga tcgtgaagcg
     9841 ccttgccttg atcagccagc aatctggccg tggtcttgtc tttcaccagc caaaccccgg
     9901 gggcaagcag gcggatcagg aaggcgtgat tggccaccgc cttgcccgtg gcatcaacca
     9961 gcgccatctc gttgccatgc gccaccgcga tcagcgagcc atcggtcatg ggcacacgcg
    10021 tggcttgcag gaccgagggc gggaccaccc acttccccga ggcatccaga tacccccaga
    10081 cgccggtgaa gcggccggtg cgttgcatgg ccggtatcca ctttccctgt tgaggcgcga
    10141 cttccatgac gtccgtcggc agcgccagtg actttccatc gcgaacccgc cacgccagcg
    10201 gctgcacggg aacctgcaat accgccacgc cgttgtcccg gccaaacccg tcgtattcct
    10261 cgcgtgtacc gggagcaagc tgcttgatca ccgtccccgt gcggtcgatc aacagttccc
    10321 gctccctctg ggccaccaag gcaacgccct ggtcgccgaa cgcagtggcc gaatcatagg
    10381 tcgcggggat cacccaattc ccctgcttgt cgatatagcc ccagtgtttt ccatcttcgg
    10441 cggccgccag cccgtgtgag aacggctgcg cgtcggtgaa ccggggcgca atactcaact
    10501 gtcccgattt gtcgatataa ccccacagga cccgcccgcc gtgatcggcc cccaactgca
    10561 gcgccgccag accatcgtga aatgactgaa agcacagttc agggcggatg ctgtcatcgc
    10621 acatctgggt gggcatggtc ctggccaacg cgcccgcgag aggcatggag gcggcaaaca
    10681 gggccaccag caatcgatgc tgaaaaagaa ctcggcaagt gatggtgttt ctcccggttg
    10741 tcgccttgcc caggacacag ggcgggtacg ccgcgcgaac gctcgcttcg ggcaagcgca
    10801 ccgcagcgct ggccaatgtg aaaaacccgg attttaccgc gcgccgcctg ataagatcta
    10861 cataagatct acagtccatg gcggtacgac gccctgcatg acacgcaccc atatgggcgg
    10921 ggcaaggcga atgacgttct ggacggaact ccgtcaacgc gggatatgca tcaccagtaa
    10981 ccgcttgagg tcgtccgaga gcacggcccg aatgccgaag gaccgtgtca tgtcttcgta
    11041 ggccaggaag cgcatgctca gacgcccacc ctcgacgtca aaagaataca acgtgtcgtc
    11101 cggatcgcca ttgttcctga gactgatgcc acgcgcacaa ctgtcgacca acagcgaggc
    11161 ggtcaccttg ggtgacttgt gacggatgtc cagcacgtct aggtacgtct ccacacccgc
    11221 gccacacaat cccatgccac gacccgcgcg agttcgttgc gttgcaataa aaagcagatc
    11281 accccggctc tcgtaaggct gcagcgaatt ggggagaaag cgaatgcggg tcgcggctcc
    11341 caccagggac ggatcatgcc cgcgcggaat acgggaaacg acggccgtgc tggccgcgtg
    11401 ttgaacctgg atgtcttgcg cggcctggcg ataggtaagt gtgtaggttt tcccagcctc
    11461 ggtcgtgatg gatgacacga cgtcgccatt gggaagttgt ttgggggcgg aggaatcagt
    11521 gatggctgcg gcgcccgccg tcggtggcga gacgaagaac attgccgcgc accacacgct
    11581 cgccagcagc acaatttgcc accgaaggcg gttccgtgca agcgatgtgg caggggcgcc
    11641 cggactcgaa ccggaaacaa cggagtcaga gtccgtcatg ataaccattt caccacgccc
    11701 caattggaac gcaaaattat atatcgtttc ggcacacgcg ttattcgatg tctatcgcat
    11761 cgagcgactt gaccagatcg tccagggctt tcaactgcct taaaaacggt tccagcttgt
    11821 cgagcggcaa ggcgctgggg ccgtcgcact tggcgtgatc cggatcggga tgcgcttcaa
    11881 gaaacacccc ccccagcccc accgccacac cggcgcgggc cagttcggac acctggctgc
    11941 gccgcccgcc cgacgccagc cccaaggggt cgcgttgctg cagggcgtgg gtcacgtcga
    12001 acaccaccgg caacccgccg catgcctttt tcatgactcc gaaacccagc atgtcgacca
    12061 ccagattgtc atatccgaaa cacgcaccac gctcgcatag caggatgcgg tcattgccgg
    12121 cttcgtggaa tttctcgacg atattcagca tctggcccgg actgatgaac tgcggcttct
    12181 tcgcgttgat gggcttgccc gtgcgggcca gcgcggtgac gaggtccgtc tggcgggcga
    12241 ggaaggcggg tagctgcaag acgtcggcca cctgcgccac ggcggcggcc tgccccggtt
    12301 cgtgtacgtc cgtcagcacg ggaaccccgt gctccgcctt gaccttgtcg agaatcctca
    12361 ggccctcatc caggccgggg ccgcgatagg agcgtatcga tgaccgattc gccttgtcgt
    12421 acgaagcctt gaacacataa ggaatgccca gcttgttcgt gacgcgcacg tactcgtcgc
    12481 aggcgcgcaa ggccagatct tccgactcca gcacgttgat accgccgaac aggacgaagg
    12541 gacggtcatt cgcacagacg atcccgtgcg tgatttctac ggatttcatg agttggactc
    12601 tcgcggcaaa tgtcaacgga gggtgtggaa cgagcaagca aaaatggtac acggtatcga
    12661 gacgcgacgc agcgcttgcg ccgccccgtc gcgccattcc cgcaggccac atccaggcgc
    12721 accgcgcgca ctaagcctcg gtcgccgccc cgcgccccgc attgcggctg tacacagtgg
    12781 gcgccacacc catcgccctt ctgaacatcg tcgagaacga cgatggattt tcgtacccca
    12841 tgtccagcgc gatgcgcgtc acgctatcgc ccagggaaag tcgcgacatc gcgaccagaa
    12901 cacatgcttg ctggcgccag ttgatgaacg tcatgccggt gttcgccaga aaccgccggt
    12961 agaacgtgcg ctcactgaca tgcagacggg ccgcccagtc ggcgggcgcc gacgcttgcg
    13021 tgggagattc gaagaagaca tggcacaaag ccgcgaggtg cggctcggac ggcatgggca
    13081 gatgcaagga gacctcgggc tgcgcaccgg cctcgcacag caccatgtcc atgagcctgc
    13141 cgtcacgggc atcgcattcg tacagcagag gcaccttcat cgcggcggcg atcagctcgc
    13201 gcaacagcgg cgacacctgg atcacgcggc atttttcggg gggctgcgcg agcgcgcctt
    13261 cccgcacata gaggctgcgc gtggtgacat tgatcgtgct gacccggtgc acgacgtccg
    13321 ccggtatcca caccgcgtac cccggcggca cgatccaggc cccatacttc gtctccagtc
    13381 gcatgatgcc cgtggtcgca tgcaacaact gcacgcgctt atgcgtatgc gcgggaatga
    13441 catatccagt ggaatgcgcg gtttccacca cgatcacgtc gcgatcggtg tcctcgatca
    13501 ggtcgatcgc ccggggcact acaacgggca gaggcgcggc atgggttgtg aacatcgcga
    13561 ttggcagaaa ttcaatgatg aatgtcgaat attatagaga cagtcaaaac acgccatctt
    13621 agaatgcaat ggaaaatcag gatttacccc gcacacacct attgttttgc aacaaaacga
    13681 aaccatgaac aacgcagcgg ccacgaccct caagcccgca accggcaccg gcatctcata
    13741 cggcgttctg ggcgcgatca gcaccacgca catgatcaac gacatgatgc agtcggtgct
    13801 gctggcgctc taccctgtgc tgcaaggcaa tttcaaactc agcttcgtgc agatcgggtt
    13861 catcaccgtc gccttccagt tttccgcgtc gctgctgcaa ccgatcattg ggcgcttcac
    13921 cgataaacgg ccgcagccct attcccttcc catcggcatg acgttcacca tggtggggct
    13981 cttgttgctg tcgcgcgcct ggaactaccc atgcgtgctc gcggccgccg ttctcatcgg
    14041 tatgggctcc tcggtgttcc atccggaatc gtcacgcgtt gcgcgcatgg cgtcagccgg
    14101 gcggcatggc ctggcacagt ccatattcca ggtaggcggc acactgggat cctccatcgg
    14161 tccgttgctg gccgcattgc tcatcgtgcc cctggggcaa cccagcgtgg cctgggtggc
    14221 cctggccgcg cttgtcggca tcatcatcct gctgcaggtc agccgctggt attcgcacaa
    14281 cctcaccagt gtgcggggcc gatcaagcct ttcgcgcacc gacaccggcc tgacgtcaaa
    14341 acaagtgcgc tcgggcctga cggtgctgtt gatcctggtg ttctcgaaat tcctgtacct
    14401 gtccagcctg aacagctact tcaccttcta cctgatgcac catttcgggc tgaccatcca
    14461 gcacgcccag tattgcctgt tcgttttcct gtttggtgcg gcgctgggca ccatggtcgg
    14521 tggtccggtc ggtgaccgca tcggacgcaa gcgtgtcatc tggggttcca tcctggggtc
    14581 cgcgccgttc gccctgatac tgccctacgt gaacctgccc gccaccatcg tcctgatttt
    14641 cttcattggc ctgatcctgt cctcggcttt tccagccatc gtggtctacg gccaggaact
    14701 cgtcccgggc aagaccggca ccatctcggg cctgttcttc ggactggcgt tcggtatcgc
    14761 cggcattggc gcggcggccc tgggcgccct ggccgattcg ctcagcatcg agttcgtcta
    14821 ccatgtgtgc gcgttcctgc cactgctggg catcgtggcc ttcctcctgc ccgacatgcg
    14881 cggtggcaag aaagctgcgg cgtgatcgga agccaagcca aaaagggttc ggcggttcag
    14941 ccgaaccgcg tggtgctcta ccgctgaacg ccgtatcaag gccaccccgg caagagggag
    15001 aagtccaggc agcgggcaac ggccgacacc aattccgtac catggcgagg gtctggggga
    15061 aacctcggca cctttcccag taaaggcgct tcaatacgcg acgccagcgc atcaacggtt
    15121 gcatcgctgt tcagcaaccc cacgtcaacg gtgttggcga cccaacccgc cagcggcaac
    15181 ccacgggcgg cgatagcctc cacggtcagc agcgtgtggt tgatgcagcc aagacgcaac
    15241 ccaaccacca gcacgacagg caaattcaat ttttgtgcaa gatccgcggt gtcgacaccc
    15301 tggttcaagg gtacccggaa accaccgacg ccttcgacca ccaccgcgtc ggcaagctcg
    15361 caaatacgcc ggtaacaggc gaggatgtgt ggcagttcta tcgtacggcc ttcccgccgc
    15421 gccgcgatat gcggcgccgt cggctggcga aacagatagg gcgttaccag ctccgtgggc
    15481 agcgccacgc tggcctgtgc cgtcaagctg tctgtgtcct cgttatgcca ggtgccgtcg
    15541 cgcagctccg ccccggcggc gacaggcttc atgccagcgg tcctgacgcc ggttcgcgtc
    15601 aacgcataca acaagctact ggcggtaagg gttttaccga tctcggtatc ggtgccagcg
    15661 acgaaatagg cgtgcttcat atctgatcac gcatgagcga tcacctcttc aaacacggac
    15721 gtcacgcgtg acgccagcat ctcacattcc atctcgtcga gaacatacgg cggcatcagg
    15781 taaaccgtgc ggcctatcgg gcgcagcaat aattcatttt caagcgcctt ggtgaaaaag
    15841 cgccgcgaaa aaccagccgc tgcagcggaa tcgtctatcg cgacgtcaaa tgcccagatc
    15901 atgcctcgat ggcgaaaatc gcgcacacgc gggtggtgcg ccacgccctg caaccccgcc
    15961 gtgatgcgcc gcgcacgcgc ccgattcgca ttcaggacat cgtcctgctc gaaaatctcc
    16021 agagtcgcca acgcggcgct gcacgccagc ggattgcccg tgtacgaatg tgaatgcaga
    16081 aaaccacggg taacgtcggt gtcataaaag gcttggtaga tgtcatccgt cgtcatgacc
    16141 agcgacaacg gcagatagcc gccactgatg cctttcgaca gacacaccat atcgggccag
    16201 actggtagcc cgtcaacgcc gatcgcggtg gcctgctcgg cggcgaaaaa cgtgccggtg
    16261 cgcccacaac ccacggctat ctcatcaaag atcaggtgaa cgtcgtagcg atcacacaac
    16321 gcacgcacct cggacagata caccggatca tgcatcgcca tgcctgtcgc gcactgcacc
    16381 agcggctcga tgatgactgc cgcaatgtga ctgctgcgct gccgcagcag cgactccaac
    16441 gcattggcgg cgcggcgcgc gacgtccgtg gcggtttcgc cattccgggc ggcccgcgcg
    16501 tccggcgaca tgaccacatg cgcccgtcgc agcaacggat cataggcatc acggaacaag
    16561 gcgacatccg tgacagccag cgcgccgatg gtttccccgt gatagctgcc ctccaggcag
    16621 acaaattcgt gtttggaggt gtggccggca ttacgccagg cgtggaaact catcttcagc
    16681 gcgatctcta cggcggacgc gccatcagag gcgtaaaaac aatgacccag ccgattccca
    16741 gtgcgttcag ccaggcattc cgacagacgg atcaccggtt cgtgcgtgaa tccggccagc
    16801 atcgcatgtt caagtttttc cagctgtgcc gcaaccgccg cgttgatgcg gggattggcg
    16861 tggccgaaca gattcaccca ccaagagctg atggcatcca gatagcgctt cccatccacg
    16921 ccatacaacc aggcgccgcg cccgtgacta atcggcacga gcggaacggt ttcatgatgc
    16981 tgcatctgcg tgcagggatg ccagacgctt cgcaggctgc gccgcaccat gtccgaccgg
    17041 ggtgctggcg atacattctt taccaatgtc ggctcctcga tgttttcttt ccgttcataa
    17101 aaagtggcgg gcctcatccg gcgtacctag tgcggtgttt gacgccatca agcgtcaaac
    17161 actgcactga tatgtattga ccttgtcaac tacgttatct cttttccccg aacgcgaagc
    17221 caatggatta gcgagccgcg ttcgggcatt taagcgcaat gcgctgagag cagagcgggc
    17281 ggcatatacg acggttgatc agt
//
LOCUS       DASTNK010000099         8525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_69509, whole genome shotgun sequence.
ACCESSION   DASTNK010000099 DASTNK010000000
VERSION     DASTNK010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8525
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69509"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFR20_07575"
     CDS             complement(<1..362)
                     /locus_tag="VFR20_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0230415.1"
                     /translation="MRRIVLKTLALGLAGVLGTLPAWSQAKPVELEFYYPVAVGGPIT
                     KIVDKSVAEFEKANPDIKIKAIYSGSYQDSVAKALTAQKGGNPPQVAVLLSTDMFTLI
                     DQDAIVPINKFIKTDADK"
     gene            complement(376..1431)
                     /locus_tag="VFR20_07580"
     CDS             complement(376..1431)
                     /locus_tag="VFR20_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0230416.1"
                     /translation="MSSVVLENVSKQYGTAQAAVSDICFEVPAGSFTVLLGPSGCGKS
                     TTLRMIAGLDTPTSGRIHIGPVDVTDLAPAKRRISMVFQSYALFPHLSVRENILFGLK
                     VRKEPVRDFDRRLKNVADLLGLSQLLDRKPSQLSGGQQQRVALGRAVISEAPVCLMDE
                     PLSNLDAQLRHEMRREIRALQQNLGITMVYVTHDQTEAMSMADQVVLLRGGRIEQHDT
                     PDRLYAYPRTEFAARFIGTPPMNLLGLTDDGARIAGASLALTSPHGARARKLGIRPEH
                     ISIIGEGGVEACVEGVEYFGADTIVVCSVGDSVGVTLRAAGHLRAAPGQAIRLAWPQH
                     AQHFFDASGLAIDTAHH"
     gene            complement(1454..2242)
                     /locus_tag="VFR20_07585"
     CDS             complement(1454..2242)
                     /locus_tag="VFR20_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HEU0230417.1"
                     /translation="MLIAQISDLHVQEQPGLDGVLASDHLRRAIDALNQFDPRPDLVV
                     MTGDLTDHGRTAEYDILRAAIGELEIPYLLIPGNHDSPARLRDAFPELAYLRSGGDFI
                     QYVDDSWPVRIVALDTTVPERPHGALCPARLKWLSDRLAEQPGRPTVVIMHHPPFETG
                     MRQMDAIGLLEGRDEFTRIIGQHANIERVLCGHLHRTIVCRVGNTVACACPGTAHQVE
                     LDLTDPGRLAAYFEPPGYQLHWVRAGATVTHHAVIGRYDGPYYE"
     gene            2433..2996
                     /locus_tag="VFR20_07590"
     CDS             2433..2996
                     /locus_tag="VFR20_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230418.1"
                     /translation="MNIQLSVDGVVTDIPGHVVSRLALCLDGTAPSQRKALILLAHHG
                     DYEVRAAVASKPGLSKECYLRLANDASVDVLSSLLRNPSFHCYALMGAYQKIVERDPR
                     LRHELSMCLDMVKAELCLELGRWLISLDDYKVRWAMANSIRTPAALLRLLADDETPCV
                     AETARHRLAGIEGRPITTPTQSLRSEW"
     gene            complement(3015..3797)
                     /locus_tag="VFR20_07595"
     CDS             complement(3015..3797)
                     /locus_tag="VFR20_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0230419.1"
                     /translation="MSGDTTEKLRAEGIHKRFGENEVLKGLSLTAHRGDVISIIGSSG
                     SGKSTFLRCINFLETPDEGRVVILGEEVRTRRDRRGQLAAIDKQQLQRLRTRVSMVFQ
                     HFNLWSHMTALDNVTAAPVNVLGLDHKAAVERAEHYLSKVGLAPTVAGKYPAHLSGGQ
                     QQRVAIARALALEPEVMLFDEPTSALDPELVGEVLKVMQTLAEEGRTMIVVTHEMGFA
                     RNVSNRVMFLHQGRVEEEGTPADVLGAPRSPRLAQFLSGSLK"
     gene            complement(3883..4623)
                     /gene="hisM"
                     /locus_tag="VFR20_07600"
     CDS             complement(3883..4623)
                     /gene="hisM"
                     /locus_tag="VFR20_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisM"
                     /protein_id="HEU0230420.1"
                     /translation="MIDVFRQYWQQYLGAGDPGHLSGAAMTLWLLVLTMAIAFAMSVP
                     LSVMRASSNKYLSRPVQLYTFVFRGTPLYVQLLIVYTGFFSLNVVRHTPMLAEFFRSG
                     FNCVIVAFSINTCAYLTEVLAGAIRSIPAGEIEAARAFGFSRATLYTRLILPSALRRA
                     LPYYSNEVILVLHSTSIAFTATVPELLKVARDVNSATFASFSAFGIAAVFYAIMAMAL
                     VAGFRLLERRWLAFLKPQSLARALGARN"
     gene            complement(4620..5306)
                     /locus_tag="VFR20_07605"
     CDS             complement(4620..5306)
                     /locus_tag="VFR20_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522525.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0230421.1"
                     /translation="MFYGYGALVLQGTLMTLALTVLSVLFSTLLGVVGALCKTSESRV
                     LRGLAVIYTTAIRSVPDLVIMLLVFYNFQILVNWLCALLGVAQIEINAFGAGVITLAF
                     IYGAYMTETFRGAFESVPRGEVEAGFSTGMSNWQVFRLITLPQMIRFALPGYSNNVQV
                     VIKSTALVSILGLVDIIGITQQVGRSSQHLFYFNVLAGLIYLAFTCATLIVLSYVGKR
                     FNAGVKEALL"
     gene            complement(5352..6131)
                     /locus_tag="VFR20_07610"
     CDS             complement(5352..6131)
                     /locus_tag="VFR20_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0230422.1"
                     /translation="MTKILVAALLSACAFAASAQSGPVIRYGVDPTFAPYESVNAQGK
                     MVGIDIEIGDAICQHLHATCSWTKINFDGAIPALKAKKVDGILSGMTITEQRKKQVLF
                     STPLYSSPSRMMVPKGVTLDTTVASLKGKTVGIVQGTTQAAYANKHWKGKGVNLVSYQ
                     NDDLAKQDLALGRIDATLQDSAAAQSFFDTAAGKNFHLTGTAVDDPIVFGHGVGIGMR
                     LGDTKLKNEIDGAIAAMKKDGTYQKILSHYAKYGLVAPKGE"
     gene            6385..7494
                     /locus_tag="VFR20_07615"
     CDS             6385..7494
                     /locus_tag="VFR20_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HEU0230423.1"
                     /translation="MQDDTSPTDVTYGATRARFLEAAERCGARLTHHLHPLKGMNGED
                     LHTDVAVVAAPGTRKWLVSVSGTHGVEGYYGSLCQTRYLDHLHARQADPQVGLLMVHL
                     INPWGTSWKRRVNEDNMDLNRNYLDFDKPLPRNPLYETVHDCFRKEAVEDPGRERARR
                     LWRDKVDEMGLTALMNHVGAGQYLHQDGLYFGGFGPSWSNTTLRAIMREHLAGCTDAI
                     SFDLHTGAGAYGHPMLMAIAEHDYPGMEDARRLYGAWLYTVLTSASQTTDTGISAAAT
                     GYTSQAMIDLMQGKRFTQLVIECGTYKEGVGHQALLDDHFLHLRGEPAGPEFERVKKS
                     LTEFFYPDDADWRELVWVRTRQIFDRALAELADRH"
     gene            7570..8511
                     /gene="panE"
                     /locus_tag="VFR20_07620"
     CDS             7570..8511
                     /gene="panE"
                     /locus_tag="VFR20_07620"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEU0230424.1"
                     /translation="MRWLIVGAGALGGYFGGRLLQAGEDVTFLVRPRRMAQLGTTGLV
                     IKSPLGDAHIPSPPCVLAQDLAAPYDMVVVGCKAYDLTDAMASFAPAVGPDTGILPLL
                     NGMRHLDQLSARFGASRVLGGLCIISAALDADGAVQHYNNLHTLTYGELDGSRSDRMR
                     AMEAAFEPANVTARASNVILQEMWEKWVLISAAAGATCLMRAAICDILEVGAIDLIIG
                     ILDDCAAIAAANGFAPRPEARERMVAFLTAPGSTMTASMMKDMERHARIEGEHIIGDL
                     ISRVGPGTSAPVLLRTVHAHLRAYEARRAREAATPPT"
BASE COUNT         1674 a         2691 c         2630 g         1530 t
ORIGIN      
        1 ttcttgtcgg cgtcggtctt gatgaacttg ttgatgggga cgatggcgtc ctggtcgatc
       61 agggtgaaca tgtcggtgga cagtagcacc gccacttgcg gggggttgcc gcctttctgt
      121 gccgtcagcg ccttggcgac cgaatcctgg tacgagccgg aatagatcgc cttgatcttg
      181 atgtcggggt tcgctttttc gaattccgcc accgacttgt cgacgatctt cgtgatgggc
      241 ccgcccacgg cgaccggata atagaattcg agctccaccg gtttggcctg ggaccacgcg
      301 ggaagcgtgc ccaggacacc agccagcccc agcgccagag ttttcagaac gatgcgtcgc
      361 ataaggtaat ccccctcagt gatgggccgt gtcgatggcc aggcccgaag cgtcaaagaa
      421 atgctgtgcg tgttgcggcc aggcgagtcg aatcgcctgg cctggcgcgg cgcgcagatg
      481 gccggcggcg cgcagcgtga cacccacgct gtcgcccacg gagcatacga cgatggtgtc
      541 ggcgccaaaa tattcgacac cctccacgca agcctcgacc cctccttcgc cgatgatgga
      601 gatgtgctcg ggccggatgc caagcttgcg cgcccgggcg ccatgcggag atgtcagagc
      661 caggctggcg cccgcaatcc gggcgccgtc atccgtcagc cccagcaggt tcatgggggg
      721 cgtgccgatg aaacgcgccg cgaattcagt gcgcggatag gcatagagcc gatcgggcgt
      781 gtcgtgctgt tcgatccgcc cgccccgcag caacaccacc tggtcggcca tgctcatggc
      841 ttcagtctgg tcgtgcgtga cgtagaccat ggtgatgccc agattttgtt gcaacgcgcg
      901 aatctcgcgc cgcatttcgt gacgcaactg agcatcgaga ttggaaagcg gttcgtccat
      961 caggcagacg ggcgcctcgg aaatgacagc gcgtcccagc gccacacgct gctgctggcc
     1021 gcccgacaat tgagagggct tgcggtccag caactgcgac aaacccagca ggtcggccac
     1081 attcttgagg cggcgatcga aatcgcgcac gggttccttg cgcaccttca agccgaacag
     1141 gatgttctcc cgcaccgaca gatgcgggaa caatgcatac gactggaaca ccatggagat
     1201 acgacgcttg gcgggcgcca gatcggtgac gtcgaccggt ccgatatgaa tacgcccgga
     1261 ggtgggcgta tcgagcccgg cgatcatgcg caaggtggtg gatttgccgc agcccgaggg
     1321 gccgagcagc accgtgaacg accccgccgg cacttcgaag cagatatcgc tcacggccgc
     1381 ctgggcggtt ccatattgtt tggagacgtt ttcgagcact acggaagaca tggcatcaat
     1441 cagaatggaa gtgtcattcg taatacgggc catcgtaacg cccgatgacg gcatgatggg
     1501 tgacggtggc accggcgcga acccagtgca actgatagcc cggcggttcg aagtaagccg
     1561 ccaaccgccc cggatcggtg agatcgagtt cgacctggtg ggccgtgccg gggcaggcgc
     1621 aggccaccgt atttccgacc cggcagacga tggtgcgatg caaatgcccg cacagcacgc
     1681 gctctatgtt ggcgtgttgt ccgatgatgc gggtgaattc gtcgcgcccc tccagcaatc
     1741 cgatggcgtc catttgccgc atgcctgttt caaacggcgg gtggtgcatg atcactacgg
     1801 tgggccgacc gggctgctcg gccaggcgat cagacagcca cttcaggcgc gccgggcaca
     1861 acgcgccgtg cgggcgttcc ggcacggtgg tgtcgagcgc cacgatacga accggccacg
     1921 agtcgtcaac gtactggatg aagtcgccac cggagcgcaa ataagccaat tccgggaagg
     1981 cgtcgcgcag ccgcgcgggg ctgtcatgat tgccgggaat gagcagatac gggatctcga
     2041 gctcgccaat ggcggcgcgc aggatgtcgt attcggcggt gcggccatga tcggtcagat
     2101 cgcccgtcat gacgaccagg tcggggcgcg gatcgaactg attcagggcg tcgatggcgc
     2161 ggcgcagatg gtccgacgcc aagacgccat ccaatcccgg ttgctcctgc acgtgcaggt
     2221 cactgatctg ggcaatcaac acttgagatc ctgtgaacaa gtatttgaaa acgatttcat
     2281 tctacctgcg aaccatgacg ggattattgc agtgattacc ctaatcgacg gcgcattttc
     2341 gtggcgtttt tctccgtgat atgagacgct atcgctgtac ggacggtcta ccatgccggc
     2401 acgccaggaa gctgtcgcat ggggggcatc aaatgaacat ccaactatca gttgacgggg
     2461 tcgtcaccga catcccgggg catgtcgtca gccgtctggc gctgtgcctg gacggcaccg
     2521 ccccatccca acgcaaggct ctcattcttc tggcccacca cggcgactat gaagtccgcg
     2581 ccgccgtggc cagcaaaccg ggactctcca aggaatgcta cctgcgattg gccaatgacg
     2641 cctccgtgga cgtcctgtcc agcctgctac gcaatccatc cttccactgc tatgcgctga
     2701 tgggcgccta ccagaagatc gtggagcgcg atccgcgcct gcgacatgaa ctgagcatgt
     2761 gcctggacat ggtcaaagcc gagctgtgcc tggaactggg acgatggctg atttcgctcg
     2821 atgactacaa agtgcgctgg gccatggcga attcgatccg cacccctgcc gcgttgttac
     2881 gcctgttggc cgacgacgaa acgccatgcg tcgccgaaac agccaggcac cgactcgcgg
     2941 gcatcgaagg ccggccgatc acgacaccaa cacagagcct tcgatcagag tggtgaaaag
     3001 ccgatacccg atacttactt caggctgcct gacaaaaact gtgccagacg cgggctgcgc
     3061 ggcgccccca acacatcggc gggggtcccc tcttcctcca cgcgcccctg gtgcaggaac
     3121 atgacgcgat tggacacgtt gcgcgcgaaa cccatttcgt gtgtgaccac gatcatggtg
     3181 cgcccttctt cggccagcgt ctgcatcacc ttgagcacct ctcccaccag ttctgggtcc
     3241 agcgctgagg tgggttcgtc gaaaagcatc acttcgggct cgagcgccag cgcgcgcgca
     3301 atcgccacac gctgttgctg gccgccggaa aggtgtgccg ggtatttccc ggcgactgtc
     3361 ggcgccaacc cgactttact caggtagtgc tccgcgcgct cgaccgcggc cttgtgatcc
     3421 aaccccagga cattcacggg ggcggccgtg acattgtcca gcgcggtcat gtgcgaccac
     3481 aggttgaaat gctggaacac catggacact cgcgtgcgca aacgctgcaa ttgctgcttg
     3541 tcaatcgcgg ccaactgtcc ccggcggtcg cgacgggtgc gaacctcttc gcccagaatc
     3601 accacgcgcc cttcgtccgg ggtctccagg aaattgatgc agcgaagaaa cgtgctcttg
     3661 cccgacccgc tggaaccgat gatgctgatc acatcgcccc gatgagccgt cagggacagg
     3721 cccttgagca cctcgttctc gccgaagcgc ttgtggatgc cctcggcccg aagcttttcc
     3781 gtggtgtcgc cgctcatcgc gcgcccctct tgatggcgtg gccaccgggc catcgcccat
     3841 tgcggccggg caccgttttt gccgtcatgt cgtggatcct gttcaattgc gcgcgcctaa
     3901 ggcccgtgcc agcgactggg gtttcaggaa cgccagccag cgccgttcaa gcagccggaa
     3961 acccgccacc agcgccatgg ccatgatggc atagaacacg gccgcgatgc cgaaagccga
     4021 aaacgaggcg aacgtggcgc tgttgacgtc gcgcgcgact ttcaacaact ccggcacggt
     4081 ggccgtgaac gcgatggacg tactgtgcag caccagtatg acttcgttgc tgtagtaggg
     4141 cagcgcgcgc cgcagggccg acggcaggat gagccgggtg tatagcgtgg cgcgcgagaa
     4201 tccgaacgcg cgcgccgcct cgatctcgcc cgccgggatc gaccggatgg cgccggccag
     4261 gacttccgtc aggtaggcgc aggtgttgat gctgaacgcc acgatcacgc aattgaaccc
     4321 gctgcggaag aactccgcca gcatcggcgt gtggcgcacg acattcaggc tgaagaaacc
     4381 ggtgtagacg atcagcagct gcacatagag cggtgtgccg cggaacacga acgtgtagag
     4441 ctgcacgggg cggctgaggt acttgttgga cgaggcccgc atgacggaca acggcaccga
     4501 catggcgaac gcgatggcca ttgtcagcac cagcagccac agcgtcatgg ccgcgcccga
     4561 caagtgcccg ggatcgccgg cacccaggta ctgctgccaa tattgccgga agacgtcgat
     4621 caaagcagcg cctctttcac gcccgcgttg aaccgcttgc ccacatatga cagcacgatc
     4681 agcgtggcgc aggtgaacgc caggtagatg agccccgcca gcacgttgaa atagaacaga
     4741 tgctggctcg agcgcccgac ctgctgcgtg ataccgatga tgtcgaccag gcccagtatg
     4801 gacaccagcg ccgtcgattt gatgaccacc tgcacgttgt tggaataccc aggcagcgcg
     4861 aaccggatca tctgcggcag tgtgatgagg cggaacacct gccagttgct catgccagtg
     4921 gaaaacccgg cttcgacctc gccacggggc accgactcga acgcgccccg gaaggtttcc
     4981 gtcatgtagg cgccatagat gaacgccagc gtgatgaccc ccgcgccgaa cgcgttgatt
     5041 tcgatttgag ccactccgag caacgcgcag agccagttga ccaggatctg gaagttataa
     5101 aacaccaaca gcatgatcac cagatcgggc acactgcgga tggctgtggt gtagatgacc
     5161 gccagccccc gcagcacgcg gctttcggaa gtcttgcaca acgcgcccac aacgcccagc
     5221 aatgtggaga acagcaccga cagcaccgtc agcgcgagcg tcatcaacgt gccctgcagg
     5281 acaagcgccc cataaccgta aaacatgtag atcgcccgga atgggcggcg ccaggcgccg
     5341 cctttgcccc gttattcgcc tttgggggca accagcccgt atttcgcgta atgagacaga
     5401 atcttctggt aggtaccatc ctttttcatg gcggcgatgg caccgtcgat ttcgttcttg
     5461 agcttggtgt cgcccagacg catgccgatg ccgaccccat ggccaaagac gatggggtca
     5521 tccaccgcgg tgcctgtgag gtggaagttc ttgcccgccg cggtatcgaa gaacgattgc
     5581 gctgcggcgc tgtcctgcag cgtggcgtcg atgcgtccca gtgccaagtc ctgcttggcc
     5641 agatcgtcat tctggtagga caccaggttc acgcctttgc ccttccagtg cttgttggca
     5701 taggcggcct gggtcgtacc ctgaacgatg ccgaccgtct tgcccttgag cgaggcaacg
     5761 gtggtgtcga gcgtgacgcc cttgggcacc atcatgcgcg acgggctgga atacagcggg
     5821 gtggagaaca ggacctgttt cttacgttgc tcggtgatgg tcatgcccga caggatgcca
     5881 tcgaccttct tggccttgag tgccggaatg gcgccatcga agttgatctt ggtccacgag
     5941 caggtggcgt gcaggtgctg gcagatggcg tcaccgatct cgatgtctat gcccaccatc
     6001 ttgccttggg cgttgacgga ttcgtagggc gcgaacgtgg ggtcgacccc gtaacggatg
     6061 acaggaccgc tctgggcgga tgccgcgaag gcacaggcgg acagcagcgc ggccacgagg
     6121 attttcgtca tgatggctcc ggaagaattc tgacgacgat gaagggaaaa gcgcaacacc
     6181 gcacggcgtt gcggaaactg gaatataccg tattgcgaaa accggaacat gcgacatcgc
     6241 gataaccaga atggtaagtc attgaaatgt atatacaatt cggacattcc cggatacgat
     6301 tagaattcag agtgccactg ctcattctct tcgcccggac aggcagtgcc gtccgggcat
     6361 gtgcgtttca tccagaatcc cgccatgcaa gacgatacct cccccacaga tgtgacctat
     6421 ggcgccaccc gggcgcgctt cctcgaagcg gccgaacgtt gtggcgcgcg gctgacccac
     6481 catcttcacc cgctcaaggg gatgaacggc gaagacctcc acaccgacgt ggcggtcgtg
     6541 gcggcgccgg gaacgcgcaa gtggctggtg tcggtttccg gcacccacgg agtggaaggc
     6601 tattacggtt ccctgtgcca gacgcgatac ctcgaccatc tgcacgcgcg gcaggccgac
     6661 ccgcaagtcg gcctgctgat ggtgcatctc atcaatccat ggggcacgtc ctggaagcgg
     6721 cgcgtcaacg aagacaacat ggatctgaat cgcaactacc tggatttcga caaacccctg
     6781 ccccggaacc ccctgtacga aaccgtgcat gactgcttcc gcaaagaagc ggtcgaagac
     6841 ccgggtcgcg aacgggcccg gcgcctgtgg cgagacaagg tcgacgaaat ggggctgacc
     6901 gccctcatga atcacgtcgg cgcgggacaa taccttcacc aggatggcct gtatttcggc
     6961 ggcttcggcc cttcgtggtc gaacactacg ctgcgcgcga tcatgcgcga acatctggct
     7021 ggctgcaccg atgccatcag cttcgatctg cacaccggcg ccggcgctta cggccatccc
     7081 atgctgatgg ccatcgccga acacgactac ccaggcatgg aagacgccag gcgcctttat
     7141 ggcgcctggc tctacacggt cctgaccagc gccagccaga ccaccgatac cggcatctcc
     7201 gcggccgcca cgggctatac gtcgcaagcc atgatagacc tgatgcaggg caagcgcttc
     7261 acgcagttgg tcatcgaatg cggtacgtac aaggaaggcg tggggcatca ggcgctgctc
     7321 gacgaccatt tcctgcatct gcgcggcgag cctgcggggc cggaattcga gcgcgtcaag
     7381 aaaagcctca cggaattttt ctaccccgac gacgccgact ggcgcgagct ggtctgggtt
     7441 cgaacccggc agatcttcga ccgggcgctg gcggagctgg cggaccgaca ctagtgcctg
     7501 gacacccgaa cctccagctc gggacatcac ggtgcggttt gagatttgat tgacaacgga
     7561 ggaatcatca tgcggtggtt gatcgtgggc gcgggcgcgc tgggcggcta tttcggcgga
     7621 cggcttctgc aagccggcga agacgtgacg ttcctggtgc ggccacgacg catggcacag
     7681 cttggcacaa ccggcctggt catcaagagt ccgctcggcg acgctcacat cccctcacca
     7741 ccttgcgtgc tcgcccagga tctggcggcc ccctacgata tggtggtggt gggctgcaag
     7801 gcctatgact tgaccgacgc catggcatca ttcgcccccg cggtggggcc cgataccggc
     7861 atcctgcccc tgctcaacgg catgcgtcac ctggaccagc tttcggctcg cttcggcgcg
     7921 agccgcgtgc tcggcggact gtgcatcatc tcggccgcct tggatgccga tggcgcggtc
     7981 caacactaca acaacctgca caccctcacc tacggcgaac ttgacggatc gcgctcagac
     8041 cgcatgcgcg ccatggaagc cgcgttcgaa cccgcgaatg tcaccgcgcg cgccagcaac
     8101 gtcatcctgc aggagatgtg ggaaaagtgg gtgctcattt ccgccgccgc gggggcaaca
     8161 tgcctgatgc gcgccgccat ttgcgacatc ctggaggtcg gtgccatcga cctgatcatc
     8221 ggcatcctgg acgactgcgc cgccatcgct gcggccaacg gcttcgcgcc gcgcccggag
     8281 gcgcgtgagc ggatggtcgc gttcctgacc gccccgggct ccaccatgac cgcatcgatg
     8341 atgaaagaca tggagcgcca cgcccgcatc gaaggcgaac acatcattgg cgatttgatt
     8401 tcccgcgtgg gtcccggcac gtccgccccg gtcctgttgc gtaccgtcca tgcccacctg
     8461 cgcgcctacg aagcacggcg cgcaagagaa gccgcaaccc cgccaacctg agcagccgcc
     8521 acgca
//
LOCUS       DASTNK010000100         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1366788, whole genome shotgun sequence.
ACCESSION   DASTNK010000100 DASTNK010000000
VERSION     DASTNK010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366788"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..133
                     /gene="rpsP"
                     /locus_tag="VFR20_07625"
     CDS             <1..133
                     /gene="rpsP"
                     /locus_tag="VFR20_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418013.1"
                     /note="binds to lower part of 30S body where it stabilizes
                     two domains; required for efficient assembly of 30S; in
                     Escherichia coli this protein has nuclease activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU0230425.1"
                     /translation="VGNEGQENLRISLDRVQYWSDNGAQLSPAVTRLVKEYSAKVAA"
     gene            173..787
                     /gene="rimM"
                     /locus_tag="VFR20_07630"
     CDS             173..787
                     /gene="rimM"
                     /locus_tag="VFR20_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248923.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU0230426.1"
                     /translation="MPDTRSPAVPDDLVELGRVVSAYGVKGWVKIQPYSADAGVLLNA
                     ASWWLKAPGSLARPGALSHPSSVVVLACRPQGASVVANLQGVDDRTQAESLKGCGVWV
                     PRAVFPSSDPDEYYWVDLIGCRLWGERDSVPELLGNVVEVLDNGAHAVLRVARMRPGP
                     DGEPAALEDSRGRALETLVPFVAAHVHHVDLAARRIDSDWPVDD"
     gene            804..1568
                     /gene="trmD"
                     /locus_tag="VFR20_07635"
     CDS             804..1568
                     /gene="trmD"
                     /locus_tag="VFR20_07635"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU0230427.1"
                     /translation="MRFDVVSLFPDLFEDARTQGVTGRAHVRGLWSLALWNPRDYTHD
                     AHRTVDDRPYGGGPGMVMMAEPLEAAVRDARAARAKSDDAPVILMSPTGRRFDQARAE
                     RLVEQGGAILVCGRYEGVDQRFIERCVTEELSLGDFVLSGGEIPALAIMDSAVRLLPG
                     ALNDARSAQQDSFNDTLTGLLDSPHYTRPEAYHGHAVPPELLSGHHANITLWRRRQSL
                     RLTTARRPDLIRQARERGLLSAEDEAFLKSLAPREE"
     gene            complement(1640..>2185)
                     /locus_tag="VFR20_07640"
     CDS             complement(1640..>2185)
                     /locus_tag="VFR20_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU0230428.1"
                     /translation="VAVDAGKRGADEETSISVQPYPVVNPALIDAEAEARVAELKAQV
                     EAIRALRGEMNLSPALRAPLIAQGDHGMLAANTPYLMALAKLSDVKVEAHLPDVGAPV
                     QVVGRTQLMLHVEIDVEVERSRLSKEVSRLEAEITKASAKLANPSFVERAPGAVVEQE
                     KARVAQFTETLGKVRQQLERL"
BASE COUNT          319 a          682 c          754 g          430 t
ORIGIN      
        1 ggttggcaat gaagggcagg aaaatctgcg tatttcgctt gaccgcgtgc agtactggtc
       61 ggacaacggt gcgcaactgt cgccggccgt cacgcgcctg gtcaaggaat actcggcgaa
      121 agtcgccgcc tgatcgcggg ctggccagac atttggccgg ctgctggttg ccatgcccga
      181 tacccgaagc cctgccgtgc cggacgatct ggtcgaactg gggcgtgtgg tctcggcgta
      241 cggtgtgaaa gggtgggtca agatccagcc ctattcggcc gacgccgggg ttttgctcaa
      301 cgcggcatcg tggtggctga aagcgcctgg ttctcttgcg aggccaggcg ctttgtcgca
      361 cccgtcgtcg gtggtggtct tggcttgccg gccgcagggc gcttccgttg tggcgaacct
      421 tcaaggcgtt gatgaccgca cacaggccga atccctgaag ggctgcggtg tctgggtgcc
      481 gcgcgccgtg tttccgtcgt cggatcccga cgaatactat tgggtcgatc tcatcggttg
      541 ccggctctgg ggtgagcgcg atagcgtgcc ggagctcttg gggaacgtgg tcgaggtact
      601 cgacaatggc gcgcacgctg tgttgcgtgt ggcccggatg cgacctgggc cggatggcga
      661 gcccgccgcg ctcgaagaca gccggggccg cgcgctggaa acgctggtgc cgttcgtcgc
      721 cgcccatgtt catcatgtcg atctggccgc ccggcgcatc gacagcgatt ggcccgtgga
      781 tgactgatcc tgggggtggg gcggtgcgct ttgacgttgt ttccttgttt cccgatcttt
      841 tcgaagatgc ccgcacgcaa ggcgtgacgg ggcgcgcaca cgtgcgcggc ctttggtcgc
      901 tggcgttgtg gaacccgcgt gactacaccc atgacgcaca ccgaaccgtg gatgaccgcc
      961 cttatggggg cggcccgggc atggtcatga tggccgagcc actggaagcg gcggtgcgcg
     1021 atgcgcgggc cgcccgcgcg aaatccgacg atgcgccggt tattctcatg agccccacgg
     1081 ggcgccgatt cgatcaggcc cgggcggaac ggttggtcga gcagggtggt gcgattcttg
     1141 tctgtggccg ctacgagggc gtggaccagc gcttcataga gcgctgcgtc accgaggagc
     1201 tgtccctggg ggatttcgtg ctgtctggcg gggagattcc cgcgctcgcc atcatggaca
     1261 gcgcggtccg gttgcttccg ggagccttga acgacgcccg ctccgcgcag caggattcat
     1321 tcaacgatac gctgaccggg ttgctcgaca gcccgcacta cacgcggcct gaagcgtatc
     1381 acgggcacgc tgtgccgcct gagctgcttt cgggccacca tgccaatatc accttgtggc
     1441 gccgtcgaca atcgctgcgg ctcaccactg cacgccgtcc cgatctgatt cgccaggcgc
     1501 gcgagcgcgg gctgctgtcc gcggaagacg aggcctttct aaagagtctc gcgccgcggg
     1561 aagagtgacc ccttcgggtg ggcgccagac atgcccgcat cgggggcgtt caagccactc
     1621 cgcgggcgtt tgaccggttc tacagacgtt cgagttgctg ccggacctta cccagggttt
     1681 cggtgaactg tgccacccgg gctttttcct gttcgaccac cgcgcctggt gcacgctcga
     1741 cgaagctggg gttggccaat ttggcgctcg ccttggtgat ctccgcctcg agccggctga
     1801 cctcctttga caggcgcgac cgctcgacct cgacgtcgat ttccacgtgc agcatgagct
     1861 gggtgcggcc caccacctga accggcgcgc caacgtctgg caggtgcgcc tcgaccttga
     1921 cgtcgctgag cttggccagc gccatgagat acggcgtgtt cgccgccagc atgccgtgat
     1981 cgccctgggc gatgagtggt gcgcgcaggg cgggcgatag attcatctcg ccacgcaggg
     2041 cgcggatcgc ttcgacctgg gccttgagtt ccgccacgcg cgcctcggct tcggcgtcga
     2101 tcagggcggg attgaccaca gggtagggct ggacactgat actggtttct tcatcggcgc
     2161 cacgcttgcc ggcgtcgacg gccac
//
LOCUS       DASTNK010000101         7907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_443233, whole genome shotgun sequence.
ACCESSION   DASTNK010000101 DASTNK010000000
VERSION     DASTNK010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7907
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443233"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VFR20_07645"
     CDS             <1..465
                     /locus_tag="VFR20_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HEU0230429.1"
                     /translation="DNWIMAYVHIAHDCQIGSHTVIANSVQLAGHIHIDDWAIIGGLT
                     GSHQFVRIGAHTMIGGTSSIRQDIPPYVIGAGDPFRPVGINAEGLSRRGFEPDVIATL
                     KEAYKILYRRQLGIEQAVEHIRALQESHSSARGALQVLVDFLDASTRGIARG"
     gene            462..1688
                     /gene="lpxB"
                     /locus_tag="VFR20_07650"
     CDS             462..1688
                     /gene="lpxB"
                     /locus_tag="VFR20_07650"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEU0230430.1"
                     /translation="MTPRIGLVAGEPSGDLLASRIMQGLVQRAEPAHCAGIGGPAMAA
                     QGLETWHSMHALTVFGYVDAFKRLPGLLATYFDVKRRWLAQRPDVFVGIDAPDFNLRL
                     ERVLKQAGIPTVHFVGPSIWAWRYERIHKIRAAVSHMLVLFPFEEALYQREGVPVTYV
                     GHPLASAIPATPDRQAVRRFLGIDEGARVLALMPGSRASEIRLLAPRFLQAVRLLQAD
                     DPALNVLVPMVNAQRHAEFEAVLRQNPISNCHILGVPAPDEGLAPGGKRESVVAGYDH
                     RPAAWNAMEAADAVLVASGTATLEAALFKRPMVISYVLSPMMRRLMAWKSGQSRPYVP
                     WVGLPNVLLNDFAVPELLQDDATPEALAKATQAALYDSGLAERLRQRFTGLHADLTRD
                     TAGLAAKVILEQAGSA"
     gene            1685..2290
                     /gene="rnhB"
                     /locus_tag="VFR20_07655"
     CDS             1685..2290
                     /gene="rnhB"
                     /locus_tag="VFR20_07655"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU0230431.1"
                     /translation="MTQADLFDQPPPALTAGVDEAGRGPLAGAVFAAAVILDPGLPIP
                     GLADSKVLSASRREHLADLIRERALAWHIASASVEEIDALNILQATMLAMRRACAGLH
                     PAPELALIDGNRVPRPLSCEARTIVKGDALVAAISAASILAKTARDAYCASMHHAYPQ
                     YAFDQHKGYGTALHLARLREHGPCPMHRRSFGPVKRLLEAE"
     gene            2356..3135
                     /locus_tag="VFR20_07660"
     CDS             2356..3135
                     /locus_tag="VFR20_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU0230432.1"
                     /translation="MKRISSRENPAYKRVLRISEGKERGWAVIEGIHLCRAWLDHVDQ
                     PDVALFDEDGLGPDGEQAELASRLDAHRCLMCESRSWRAVSGVEHGQGVLFVVRVPRP
                     DVPPIIDRNCIWLDRVQDPGNLGTLLRTAAAAGIRQAYLSEGCAHAWSQKVLRSAQGA
                     HFVMAVYERQELQALRARLRIPLVVTSLRDARSIDDVVLPADCAWLFGNEGRGVADDL
                     LDVADERVFIPQAATVESLNVGAAAAICLFEQRRRHRAGAA"
     gene            complement(3201..4034)
                     /locus_tag="VFR20_07665"
     CDS             complement(3201..4034)
                     /locus_tag="VFR20_07665"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEU0230433.1"
                     /translation="MKQSLPDRTVFIVSDSTGITAETFSHSVLSQFEEVNFQSVRVPF
                     IDSVEKAEAAAQRINRCAQEQNQPPLVFSTLVNPDIALKIRQSNCTFLDLFGTFVQHI
                     ETALGMKSSHSVGRSHMNGLTKQYHNRIEAINFSLAHDDGQFIQGLEQADVILVGVSR
                     CGKTPTSLYLAMQYAIKAANFPLIPEDFDRDALPATLAPYRKKLFGLSIQPGRLAEVR
                     HERRPNSEYASLRQCRYEVARAERLMRMENIPWLSTTTKSIEEISTKVLEEVGLNRQT
                     Y"
     gene            4194..6557
                     /gene="ppsA"
                     /locus_tag="VFR20_07670"
     CDS             4194..6557
                     /gene="ppsA"
                     /locus_tag="VFR20_07670"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEU0230434.1"
                     /translation="MSYVVSFEQLRMSDVDSVGGKNASLGEMISQLSGAGVRVPGGFA
                     TTADAFREFLAATGLDKRIADRLETLDSEDVRELASAGQEIRQWVIDAPFPAAFEQAV
                     RDAFAKLDADGKGSFAVRSSATAEDLPDASFAGQQETFLNVVGIDDVLHKIRHVFASL
                     YNDRAISYRVHKGYAHAGVALSAGIQRMVRSDRGSAGVMFTLDTESGFTDVVFITSSY
                     GLGETVVQGAVNPDEFYVFKPTLASGHYPIISRRIGSKLIKMEFDPERAAGLAVRTVD
                     VPVSERNRYSLTDEEIIELARYAVIIEKHYQRPMDIEWGRDGIDGKIYILQARPETVK
                     SQQGHHDVQERYRLKATGAVLITGRAIGQKIGSGRVRVVADVSEIDQVRPGDVLVTDM
                     TDPNWEPVMKMASAIVTNRGGRTCHAAIIARELGIPAVVGCANATDVLATDREVTVSC
                     AEGDEGRIYDGLIETEIEEVRWGQMPDIGLKIMMNVGNPQLAFDFSQIPNQGVGLARL
                     EFIINNNINVHPKAVLDYPNVDIDLKKAVESAARGYASPRAFFVEKLAEGVATIAAAF
                     YPKSVIVRLSDFKSNEYRKLVGGSRYEPEEENPMLGFRGASRYISAEFEECFRMECEA
                     LKKVRDEMGLTNVEIMVPFIRTLKQAERVVDLLARHGLARGENGLRLIMMCEVPSNAI
                     LAEQFLRYFDGFSIGSNDMTQLTLGLDRDSGLELLAHDFDERDDAVKFMLSRAINACL
                     EAGKYVGICGQGPSDHPDLAEWLRDQGILSMSLNPDTVVETWQNLAK"
     gene            6601..7905
                     /locus_tag="VFR20_07675"
     CDS             6601..7905
                     /locus_tag="VFR20_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0230435.1"
                     /translation="MDEQPGNIVIVGGGAGGLELAARLGGRLGPEQVTLVDKTAFHIW
                     KPSLHEVAAGTLDIHREGLSYFMLAKDRGFTFVRGEMDNLDAAGKSIGIRPVFDPYGE
                     QVLPARRLAYGTLILSVGSRSNFFGTPGAAEYTLAIDSTEQAESFRLRLLSALTRTDA
                     ARGAGDGEPLRIAIVGAGATGVELAAELAQAARNLAYYGLRHLDPARDLKISVIEGAP
                     RILSALHEKISQAAHRLLTDRGIAVETSVHVASVHADALIDTEGQRHPYDLCVWAAGI
                     QAPAFLSGLGLETNRINQVVVDASLRSSDPFIFAFGDCAQAAWANDRFLPARAQVAHQ
                     QAAFLFSRLVHGNTTGKRPSTAFRYRDYGSLVSVGHNRVVGNLMGVLTGKGLFIEGLF
                     ARLMYIGLHLMHHAALLGLLRTGSLALGRLLLKRGAPRVKLH"
BASE COUNT         1396 a         2390 c         2549 g         1572 t
ORIGIN      
        1 gacaactgga tcatggcgta cgtgcacatc gcgcacgatt gccagatcgg cagccatacc
       61 gtgatcgcca attcggtgca gcttgccggc catattcaca ttgacgactg ggcgatcatc
      121 ggtgggctca caggctcaca tcagttcgtg cgcatcgggg cgcacaccat gataggcggc
      181 accagttcca tccgccagga tattcctccc tatgtcatcg gcgcgggcga tccctttcgc
      241 cccgtgggca tcaacgccga ggggttgtcg cgacgcggtt tcgaaccaga cgtcatcgca
      301 actctgaaag aggcctataa gattctgtat cgacgtcagc tcggcatcga gcaggccgtc
      361 gagcacatcc gcgcgttgca ggaatcacac tcgtcggccc ggggcgcgct gcaggtgctg
      421 gtcgactttc tcgacgcgtc gacccgcggc atcgcgcgcg gatgactccc cgtatcggcc
      481 tggtggccgg cgagccatcg ggggatcttc tcgcttcgcg catcatgcag gggctcgtcc
      541 aaagggccga acccgcgcac tgcgccggca ttggcggccc ggccatggcg gcgcaaggat
      601 tggaaacctg gcactccatg catgcactga cggttttcgg ctatgtcgat gcgttcaagc
      661 ggctgcccgg gctccttgcc acgtacttcg acgtcaagcg gcgctggctc gcccagcggc
      721 ccgacgtatt cgtcggcatc gacgcgccag acttcaactt gcggctggag cgtgtgttga
      781 agcaggcggg gatccccacg gtgcatttcg tggggccttc gatctgggcc tggcgctacg
      841 agcgtatcca caagatccgt gccgcggtgt cgcacatgct ggtgctgttt ccgttcgagg
      901 aagccctcta tcaaagggaa ggcgtgccgg tcacttatgt gggccatccc ttggcctcgg
      961 cgattcccgc cacgcctgat cgacaggcag tccggcgttt tctgggtatt gacgaaggcg
     1021 cgcgggtgct ggccctgatg ccgggcagcc gtgcgtcgga aatccgtctg ctggcacccc
     1081 gttttctaca agcggtgcgg ttattgcagg ccgatgatcc ggcgctgaat gtgcttgtgc
     1141 ccatggtcaa cgcacaacgg catgccgaat tcgaggcggt actgagacaa aacccgatat
     1201 ccaattgcca catcctgggt gttccggcac cggatgaagg gttggccccc ggcggcaagc
     1261 gcgaatccgt cgtcgcgggc tacgatcata ggccggccgc ctggaatgcc atggaagccg
     1321 cggacgcggt gctggtggcc agcgggacgg ccacgctgga agcggcgctc ttcaagcgcc
     1381 ccatggtgat ctcgtatgtg ctttcaccca tgatgcgccg tctgatggcc tggaaatcag
     1441 ggcagagccg gccgtatgtg ccctgggtcg gtctgcccaa cgtgttgctc aatgatttcg
     1501 ccgtgccgga actgcttcag gacgacgcca cgcccgaggc gctcgccaag gccactcagg
     1561 cggcgctata cgactcaggc ctggcggagc gcctgcgaca gcgcttcacc gggttgcatg
     1621 ccgatctgac acgcgacacc gcgggcttgg ccgccaaggt catcctggaa caggcgggct
     1681 cggcatgact caggccgatc tgttcgatca gccaccgcca gcgctgacag ccggtgtcga
     1741 cgaggcggga cgcgggcctt tggccggcgc tgttttcgcc gccgcggtca tcctggatcc
     1801 ggggctccct attcccgggt tggccgattc caaggtattg agcgcgagtc ggcgcgaaca
     1861 tctggcggac ctcatccggg agcgggcact ggcctggcac attgcttcag caagcgtgga
     1921 ggaaatcgac gcgctgaaca ttctccaggc caccatgctg gcgatgcggc gtgcctgcgc
     1981 cgggttgcat cccgcgccgg aattggcgct catcgatggg aaccgtgtgc ctcgcccgct
     2041 gtcctgcgag gctcggacca tcgtcaaggg ggatgcgctg gtcgcggcga tctcggcggc
     2101 gtcgattctt gccaagacgg ctcgggatgc ctactgcgcg tccatgcatc acgcgtatcc
     2161 gcagtatgct ttcgatcagc ataaagggta tggcacggcg ctgcacctgg cacgccttcg
     2221 ggaacatggc ccgtgtccaa tgcaccgccg gagctttggc ccggtgaaga ggcttctgga
     2281 agcggaatga tcgccccggg cccccggcct tgcttcccca accatgaaac gcatcagcca
     2341 gtccacatcc cagccatgaa acgcatcagc tcacgtgaaa atccggcgta caagcgggtg
     2401 cttcgtatct cggaaggcaa ggagcgcggc tgggcggtga tcgagggcat tcatttgtgc
     2461 cgcgcgtggc tcgaccacgt ggaccagccc gacgtggccc tgttcgatga ggatgggttg
     2521 ggtccggatg gcgaacaggc tgaactggcc tcgcggctcg acgcgcatcg atgcctgatg
     2581 tgcgagtcgc gctcatggcg tgcggtatcc ggggtcgagc atggccaggg tgtgcttttc
     2641 gtggttcgtg tgccgagacc ggacgttcca ccaatcatag accggaactg catctggctg
     2701 gatcgcgtgc aggatccggg caatctcggg actcttttac gcacggccgc ggcggccgga
     2761 ataaggcagg catacctctc cgaagggtgt gcgcacgcgt ggtcgcaaaa agttctgcgt
     2821 agcgcccagg gcgcgcactt cgtcatggcg gtctacgagc gccaggaact acaggctttg
     2881 cgggctcgcc tgcgtattcc gctggtggtg acatcgttgc gtgacgccag gtcgattgac
     2941 gacgttgtac ttccggccga ttgcgcctgg ttgtttggca atgaagggcg tggtgtggcg
     3001 gatgatctcc tggacgtcgc cgatgaacgg gtgttcatcc cacaagccgc gacggtggag
     3061 tcgctgaacg tgggggcggc ggcggcgatc tgcttgttcg agcagcggcg gcgacacagg
     3121 gctggtgccg cgtagcccac tttttccggc gtacgcgcat cgctgatgaa ggcgcttggc
     3181 gatacggcct cgcgccactc tcagtaggtc tggcgattca agccgacttc ctcaagtacc
     3241 ttggtggaga tttcctcgat ggacttggtc gtcgtggaca gccagggaat gttctccatg
     3301 cgcatcagcc gctcggcgcg ggccacttcg tagcggcatt ggcgcagcga ggcgtattca
     3361 ctgttggggc gccgctcgtg ccggacttcc gccagccgtc caggctggat cgacaggccg
     3421 aagagcttct tgcgataggg agccagtgtg gcgggcagcg cgtcgcggtc gaaatcttct
     3481 gggataagag ggaaattggc ggccttgatg gcatactgca ttgccaggta caggctggtg
     3541 ggcgtcttgc cgcagcgcga caccccgacc aggatgacgt cggcttgctc cagcccctgg
     3601 atgaattggc catcgtcgtg cgcgagactg aaattgatcg cttcgatgcg gttgtggtac
     3661 tgcttggtca ggccgttcat gtgcgagcgg ccgaccgaat ggctggattt catgcccaat
     3721 gcggtttcga tgtgctgcac gaacgtgccg aatagatcga ggaacgtgca attggactgg
     3781 cggatcttca gggcgatgtc cgggttgacc agggtgctga acaccaaggg tggctggttt
     3841 tgttcctggg cgcagcgatt gatgcgctgg gccgccgcct ctgctttttc gacactgtcg
     3901 atgaagggta cgcgcaccga ctggaagttg acctcttcga attgcgacag caccgaatga
     3961 ctgaaagttt cagcggtgat gccggtgctg tcggaaacga tgaaaaccgt tcggtcggga
     4021 agggattgtt tcatgaagaa ataatggtgg gctggaaagt gaaggcggta caatcgcagg
     4081 ttaccagtca cctcctttgc cggcaaggct ggtttggtta tggcatccag ggtgctcaag
     4141 ctccctgggc gccataacca aacacgcttt tttctatcga tataggtgac tttatgtctt
     4201 atgttgtttc tttcgagcag ctccgcatgt cggatgtcga ctcggttggg ggtaagaacg
     4261 catcactggg tgaaatgatc agtcagcttt ccggggcggg ggttcgcgtg cccgggggct
     4321 tcgccaccac ggccgacgcc tttcgcgagt tcctcgccgc cacggggctg gacaagcgta
     4381 ttgccgatcg cctggaaacc ctggattccg aagacgtgcg cgaactcgcg tccgcaggtc
     4441 aggaaatccg gcagtgggtc atcgacgcgc cgtttcccgc cgctttcgaa caggcggtgc
     4501 gcgatgcttt cgccaagctt gacgccgatg gcaagggttc cttcgccgtg cgttcgtcag
     4561 ccacggccga agacttgccc gacgcctcgt tcgccgggca acaggaaacc ttcctgaacg
     4621 tcgtgggcat tgatgacgtt ctgcacaaga tccgtcatgt cttcgcgtcg ttgtacaacg
     4681 atcgcgccat ttcctaccgc gtccacaaag ggtatgcgca cgccggcgtc gccctgtccg
     4741 ccggtataca gcgcatggtt cgttccgatc gtggcagtgc tggggtcatg ttcacactgg
     4801 ataccgaatc agggttcacc gatgtggtgt tcatcacttc gtcgtatggg ctcggtgaaa
     4861 ccgtggtcca gggcgccgtc aaccccgacg aattctacgt gttcaaacct acgctggcgt
     4921 ccggccatta ccccatcatc agccgccgta tcggctcgaa actcatcaag atggaattcg
     4981 atccggaacg cgcggccggg ctggcggtgc gtaccgtgga cgttcccgtg tcggagcgca
     5041 accgctattc cctgaccgat gaagaaatca tcgaactggc tcgctacgcg gtcatcatag
     5101 aaaagcatta ccagcggccc atggacatcg aatgggggcg cgatggcatt gatggcaaga
     5161 tctacatcct gcaggcgcgt cctgagaccg tcaaatcgca gcaaggccac cacgatgtcc
     5221 aggaacgcta tcgcctcaag gccaccggcg cggtgttgat tacgggtcgg gccatcggcc
     5281 agaagatcgg ttcgggccgg gtgcgcgtgg tggccgacgt ttccgaaatc gaccaagtcc
     5341 gcccgggtga cgtgttggtc accgacatga ccgatcccaa ctgggaaccg gtcatgaaaa
     5401 tggcctcggc cattgtcacc aatcgtggcg gccgcacctg ccatgcggcc atcatcgcac
     5461 gcgaactcgg cattccggcc gtggtgggct gcgccaacgc caccgatgtg ttggccaccg
     5521 accgtgaagt caccgtgtcg tgcgccgagg gcgacgaggg ccgcatctac gacgggctca
     5581 tcgagaccga gattgaggaa gtgcgttggg gccagatgcc cgacatcggc ctgaagatca
     5641 tgatgaacgt gggtaacccg caattggcgt tcgacttctc ccagatcccg aatcagggtg
     5701 tgggtttggc gcgcctggaa ttcatcatca acaacaacat caacgtgcac cccaaggccg
     5761 tgctcgacta ccccaatgtc gacatcgatc tgaaaaaggc ggtggaatcg gccgcccggg
     5821 gctatgccag tccacgcgcg ttcttcgtcg aaaagctcgc cgaaggggtc gccaccatcg
     5881 cggccgcgtt ctatcccaaa tccgtcatcg tgcggctgtc cgatttcaaa tccaacgagt
     5941 accgcaagct cgtcggcggt tcgcgctacg agcccgagga agaaaacccc atgctgggtt
     6001 tccggggggc gtcgcgctac atttcggccg agttcgaaga atgtttccgc atggaatgcg
     6061 aggcgctcaa gaaggtgcgc gacgaaatgg gcctgaccaa cgtcgagatc atggtgccgt
     6121 tcatccgcac cctgaagcag gccgagcgtg tggtcgatct cctggcccgg cacggcctgg
     6181 cgcgtggcga gaacggattg cgcctgatca tgatgtgcga ggtgccttcc aacgcgatcc
     6241 tggccgaaca gttcctgcgg tacttcgatg ggttctccat cgggtccaac gacatgaccc
     6301 agctcacgct ggggctggat cgcgactcgg ggcttgagct gctggcccat gatttcgatg
     6361 aacgcgacga tgccgtcaaa ttcatgctga gtcgggccat caatgcctgc ctggaggccg
     6421 ggaaatatgt cggcatttgc gggcagggcc ccagcgacca tcccgacctg gccgagtggc
     6481 tgcgagacca gggtattctg tccatgtcgc tcaaccccga caccgttgtt gaaacctggc
     6541 agaacctcgc caagtaaggc ggccatccag catttcgccc cgcccggaaa caatgcaacc
     6601 atggatgaac aacctggaaa catcgtgatt gtcggggggg gcgcgggagg cctggagctg
     6661 gcggcccgtc ttggtggccg gctcgggccc gaacaggtca ccctggtcga caagacggca
     6721 ttccacatct ggaaaccctc gctgcatgaa gtcgcggcgg gtacgctgga catccatcgc
     6781 gaaggtctct cgtatttcat gctggccaag gatcgcggct tcacgttcgt gcgtggcgag
     6841 atggacaacc tcgacgcggc cggcaaatcc atcggcatcc ggccagtgtt cgatccatac
     6901 ggcgaacagg tcctgccggc gcggcggctg gcttacggca cattgattct ctcggtgggc
     6961 agtcgatcca attttttcgg gacccccggc gcggccgagt acaccctggc catcgactcc
     7021 accgagcagg cagagtcctt tcgcctgcgg cttctgagcg ccctgacgcg taccgacgcc
     7081 gcgagaggcg cgggcgatgg cgagcccttg cgcatcgcca tcgtgggcgc gggggcgacc
     7141 ggtgtcgagc tggccgccga gctggcccag gccgcgcgca acctggctta ttacggcctt
     7201 cggcatctgg atccggctcg ggacctgaag atcagcgtca tcgagggggc gccccgcatc
     7261 ttgtcggcgt tgcacgagaa gatctcccag gccgcgcacc gtttgctgac tgatcgtggc
     7321 atcgctgtcg aaacgtcggt gcacgtcgcg tccgtgcacg ccgacgcgct gatcgacacg
     7381 gagggccagc gccatccgta cgatctatgc gtctgggcgg cgggaataca agcccccgca
     7441 ttcctgtccg ggctgggtct ggaaaccaat cgcatcaatc aggtggtggt tgatgcgtca
     7501 ttgcgcagca gcgacccttt catcttcgcg ttcggcgatt gtgcgcaggc cgcatgggcc
     7561 aacgaccgtt tcctgccggc gcgggcgcaa gtcgctcatc agcaagcggc gttcctgttc
     7621 tcccggcttg tgcatggaaa cacaactggc aaacgcccgt ccaccgcttt ccggtaccgg
     7681 gattacggct ccctggtgtc ggtgggtcac aaccgcgtgg tgggcaatct gatgggggtg
     7741 ctcaccggca aggggctgtt catcgaaggg ctgttcgcgc gtttgatgta cattggtttg
     7801 cacctgatgc accacgcggc gctgctcggc cttttgcgca cgggctcgct ggcgctgggc
     7861 cgactactgt tgaagcgcgg cgcgcctcgc gtgaagttgc actagga
//
LOCUS       DASTNK010000102         6632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_443291, whole genome shotgun sequence.
ACCESSION   DASTNK010000102 DASTNK010000000
VERSION     DASTNK010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443291"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(297..1229)
                     /gene="rsmI"
                     /locus_tag="VFR20_07680"
     CDS             complement(297..1229)
                     /gene="rsmI"
                     /locus_tag="VFR20_07680"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU0230436.1"
                     /translation="MNESAEILDPGASWNRLIERVGRQSWPPATLYVVATPIGNLGDL
                     GLRAWQALSRCDVIAAEDTRTSRALLDAWGVDTPLIAAHRHNEAGAAEAIVARLGRGE
                     RVALISDAGAPAVSDPGARIVRVVREAGFQVQAIPGPSAVVTALMASGVTSDENPAFV
                     FAGFPPARSGLRQKWLRRWCAVPAPIVLFESPHKLAATIDDLAVVCGGARTVTIAREL
                     TKRFEQVTTLALADVPGWLAGDAHRAQGEFVVIVHEAPEIEHDDASDESTGRWLDAML
                     ESVSVRDAARIAAKATGQSRDALYAQALARRKSD"
     gene            1273..1761
                     /locus_tag="VFR20_07685"
     CDS             1273..1761
                     /locus_tag="VFR20_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEU0230437.1"
                     /translation="MQNGEALASHLAHLAQQKAVRARKRRLARAAPAPRIRTGVSPKQ
                     AQGGQAEERARHHLLDAGATVLARNLRCKGGEIDLVALDHGVLVFVEVRYRGSERFGG
                     AAASVNRRKRQRLVLAARYFLPTLVRRYFSGHPPPCRFDVIAQTPLATTWLKHAFAED
                     LE"
     gene            1829..2422
                     /locus_tag="VFR20_07690"
     CDS             1829..2422
                     /locus_tag="VFR20_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEU0230438.1"
                     /translation="MDMTSCMASHFDDAIDVFKASAKELAAPLAAGVDLLFGTLSNDG
                     KVLACGNGGSAADAQHFIAELVGRFERDRLPLAGVALNTDTSILTAVGNDYGFDAVFE
                     RQVTALGRPGDVLVAISTSGNSTNVLRAIEAAHDRDMAVIALTGKNGGKTTELLRESD
                     INLCVPHDRTMRIQEVHILLLHALCHGIDALLLGDPL"
     gene            2422..3132
                     /locus_tag="VFR20_07695"
     CDS             2422..3132
                     /locus_tag="VFR20_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU0230439.1"
                     /translation="MTSLTRTSNILLRACAVAIVLGTLSGCGALIVGGAAASGATVAT
                     DRRTAGAQLDDQTIEIKVSNNMTDLFGDKARVSAMSYDGWVLLTGDVPTEQDKQRAEA
                     TVSKIEKVRKVINALRVGPITPLSVRTNDVWITSKVKANLIDTKGVPSRTIDITTNRG
                     IVYMMGKVTDAEAQRAAIVASSVNGVNKVVKLFEIVSPESLQQRTSPAPIQDNSTSPP
                     STPAPAASSNGVQAMPVK"
     gene            3129..3626
                     /locus_tag="VFR20_07700"
     CDS             3129..3626
                     /locus_tag="VFR20_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU0230440.1"
                     /translation="MKKALIGAVVVVALAGAGFWQLSGTAKAAPEVTFTSLTGQKITT
                     QDLRGKVVLVKFWATSCSTCIQQMPTMIKSYDKLAPKGFEAIAVAMQYDPPNYVKNYA
                     ETRHLPFPVAIDAQGKIAKAFGDIQLTPTAFLIDKQGHIIKRYLGNYDHAAFMRTVEK
                     ALASS"
     gene            3769..4671
                     /gene="argB"
                     /locus_tag="VFR20_07705"
     CDS             3769..4671
                     /gene="argB"
                     /locus_tag="VFR20_07705"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEU0230441.1"
                     /translation="MTQQSPESSSYPSTVKADVLSEALPYIRRFHGKTVVIKYGGNAM
                     TEEQLQRSFAHDVVLLKLVGLNPVVVHGGGPQINQALKRIGKEGSFVQGMRVTDAETM
                     EVVEWVLGGQVQQDIVMMINEAGGKAVGLTGKDGCLIQATKKLMADDANPGKWLDIGY
                     VGDIARIEPAVVKALQDDQFIPVISSIGYGEDGMAYNINADVVAGKMAETLGAEKLIM
                     MTNTPGVLDKNGQLLRNLSAQTIDELFKDGTISGGMLPKISSALDAARNGVNSVHVVD
                     GRVPHCLLLEILTDRGVGTMISSH"
     gene            4688..5446
                     /locus_tag="VFR20_07710"
     CDS             4688..5446
                     /locus_tag="VFR20_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEU0230442.1"
                     /translation="MYPGRPQARRTRPTRSSQAANTEPVWLFDLDNTLHDSSREIFKA
                     IDRSMTAAVASSLDITVEAANLLRATYWKRYGATVIGMVRHHDIDASAFLRLSHQFDV
                     ASMVHAETGMAYKLGRLPGRKYVFTNAPLGYARTVLKTLKILQCFDGVWAIDDMVLQG
                     RFRPKPSAALMRQAIARLGVPAHRVILVEDTLRNLKSARQAGMRTVYVYHPGTPFSRG
                     AHGRDQYVDVRVNSIGHLLIGRRAPAESRPSPTP"
     gene            5458..6009
                     /gene="slmA"
                     /locus_tag="VFR20_07715"
     CDS             5458..6009
                     /gene="slmA"
                     /locus_tag="VFR20_07715"
                     /inference="COORDINATES: protein motif:HMM:NF007015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HEU0230443.1"
                     /translation="MPASRRAQILDTLSAMLGQADAGRVTTAALAARLAVSEGALYRH
                     FSGKAEIFEALIDAAEALIEEDLDHIATTEPDARLRLRKNLRTLRLFAERHPGLARVL
                     TGHALSGEGLDLTQRADAVIDHLRSRLAQDAAKAGPAFDVHPDATARVLTQWILGCWQ
                     RPARRNPPRTDTSLDDELAFLGL"
     gene            complement(6021..>6632)
                     /locus_tag="VFR20_07720"
     CDS             complement(6021..>6632)
                     /locus_tag="VFR20_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEU0230444.1"
                     /translation="LAGSGVGHIVLADDDIVDLGNLQRQLLHTTARVGWPKARSGRHM
                     LADLNPEVHVEALEQRLQGADLSRVVSGVDLVLDCCDNFATRHAVNRACATHGKPLVS
                     GAAIRFSGQVAVFDLRHADSPCYHCLFPEAGGAHEANCATTGVLAPLVGVIGSMQAIE
                     AIKLLTGVGEPATQRLQCFDALSAQWHEIRFERDPDCPVCSHR"
BASE COUNT         1285 a         2272 c         1997 g         1078 t
ORIGIN      
        1 tccatcgggg cctggggcgg ggccggtgta aggcgtgcgc cggattcgcc gtgggagggc
       61 gggggccttc gtcgggcggt gtctgagcgc agcctcgcga tagcgaggcg tagcgagttc
      121 agcccgacgc ccgcccggac gcggcggatc cggatgaagc acgccgtgcc ggaaccgccc
      181 caggccccga tggacagcga ctttagatac aagacagcgc cgtcatacac cgccagcacc
      241 aacatcacca acatcaccaa caacatcgaa ctccacaggc gcccgaacaa aaacccctag
      301 tcggacttac ggcgcgcgag cgcctgcgca taaagcgcgt cgcgcgattg tcctgtcgcc
      361 ttggcggcga ttcgcgccgc atcgcgcacg ctgaccgatt cgagcatcgc gtcgagccag
      421 cgtccggtcg actcatcgga ggcgtcgtca tgctcgatct ccggcgcctc atgcacgatc
      481 acgacgaact cgccttgcgc ccggtgcgcg tccccggcca accagcccgg cacatcggcc
      541 aacgccagag tggtcacctg ctcgaaccgc ttggtcagtt cccgggcgat cgtcacggtg
      601 cgcgctccgc cgcacacaac ggccaggtca tcgatggtcg cggcgagctt gtgcggggac
      661 tcgaacagca cgatcggcgc gggcaccgcg caccagcgcc gcaaccattt ctgccggagg
      721 cccgaccggg cgggcgggaa gcccgcgaac acgaaggccg ggttttcatc ggaggtcacc
      781 ccgctggcca tgagcgccgt cacgacggcg ctggggccgg gaatcgcctg cacctggaaa
      841 cccgcctccc gcacgacacg cacgatgcgc gcgcccgggt cgctgacggc gggcgcgccc
      901 gcgtccgata tgagcgcgac ccgctctccg cgtcccaggc gtgccacgat ggcctcggcg
      961 gcaccagcct cgttatggcg gtgcgccgcg atcaggggag tatccacccc ccaggcatcg
     1021 agcaatgccc ggctggtgcg cgtgtcttcc gccgcgatca catcgcatcg cgacagcgct
     1081 tgccaggcac gcaagcccag gtcgcccaga ttgcctatgg gcgtcgccac cacgtacaag
     1141 gtggcgggcg gccagctctg gcggccgact cgctcgatga gacgattcca tgacgcaccg
     1201 gggtccaaaa tctccgcaga ctcgttcata ctggcactac ccataaaatc catgactgaa
     1261 aggcgccgtc ccatgcaaaa cggcgaagcg ctcgcctccc atctggcgca cctcgcacag
     1321 caaaaagctg tgcgagcccg caagcgccgc ctggcgcgcg cggcacccgc gccgcgcatc
     1381 cgtactggcg tgtcacccaa gcaggcgcaa ggcggtcaag ctgaagagcg cgcccgccac
     1441 cacctgctgg atgccggtgc caccgtgctg gcgcgcaatt tgcgctgcaa gggcggggaa
     1501 atcgatctgg tggcgctgga ccatggcgtg cttgttttcg tcgaggtccg ttatcgcggc
     1561 agcgaacgtt tcgggggcgc agcagccagt gtaaaccgtc ggaaacgaca acgcctggtt
     1621 ctggcggcgc gctatttcct gcccaccctc gtgcggcgat acttctccgg ccaccctcca
     1681 ccgtgccgat tcgatgtcat agcccagact ccgctcgcga cgacctggct caagcacgcg
     1741 ttcgccgaag atctcgagta gcggcggcca ggccgcatac gttacgtccg ctgacacccc
     1801 cacgacacag catgagataa tattcgccat ggatatgact tcttgcatgg cctcgcattt
     1861 cgacgacgcc atagacgttt tcaaggccag cgcgaaagag ctcgccgcgc cgctcgcggc
     1921 cggcgtcgat ttgcttttcg gcacgctgtc caacgacggc aaggtgctgg cttgtggcaa
     1981 cggcgggtcg gccgccgacg cccagcactt catcgccgaa ctggtcggcc gcttcgagcg
     2041 cgaccgcctg cccctggccg gcgtcgccct gaacaccgac acctctatcc tcacggcggt
     2101 gggcaacgat tacggtttcg acgctgtctt tgaacgccag gtcactgccc tgggccgccc
     2161 cggcgatgtg ctggtggcga tttccaccag cggcaattcc accaacgtcc tgcgcgcgat
     2221 cgaagccgcc cacgaccgcg acatggccgt catcgccctc accggcaaga acggtgggaa
     2281 gaccaccgaa cttctgcgtg aatccgatat caatctgtgt gtgccgcacg accggaccat
     2341 gcgtatccag gaagtccata tcctgcttct gcacgcgctg tgccacggca tcgatgccct
     2401 cttgctcgga gaccccctgt aatgacctcg ctgactcgaa cctcgaatat tctcttgcgc
     2461 gcctgcgcgg tcgccatcgt gctgggcacg ctgtcgggct gcggcgccct catcgtcggt
     2521 ggggcggcgg cgtccggtgc aacggtggcc acagaccgcc gcaccgcagg agcccaactg
     2581 gatgaccaga ccatcgaaat caaggtcagc aacaacatga ccgacctgtt cggtgacaag
     2641 gcccgggtca gtgccatgtc gtacgacggc tgggtgttgt tgaccggcga tgttcccacc
     2701 gagcaggaca agcagcgggc ggaagccacg gtctcgaaga ttgaaaaagt ccgcaaggtc
     2761 atcaacgccc tgcgcgtcgg cccgatcacc ccgctcagtg tgcgcaccaa cgacgtctgg
     2821 atcacctcca aggtcaaggc caacctcatc gacaccaaag gggtgcccag ccgcaccata
     2881 gacatcacca ccaaccgcgg gatcgtctac atgatgggca aggtcaccga tgccgaagcc
     2941 cagcgggccg ccatcgtcgc ctcttctgtg aacggcgtga acaaagtagt aaaattgttc
     3001 gaaatcgtgt cgcccgagag cttgcagcag cgaacctccc cggcgccgat acaggataat
     3061 tcaacgtcac ctccttctac cccggcgccg gcggcttcat caaacggtgt ccaggcaatg
     3121 cctgtgaaat gaaaaaagca ctcattggcg ccgtggttgt cgtcgccctc gcgggcgcgg
     3181 gcttctggca gttgtccggc acagccaagg ccgccccgga agtcacgttc acttccctga
     3241 ccgggcagaa aatcaccacg caagacctgc gcggcaaagt cgttctggtc aaattctggg
     3301 caaccagctg cagcacctgc attcagcaga tgcccaccat gatcaagtcg tacgacaaac
     3361 tggcgcccaa ggggttcgaa gccattgccg tcgccatgca gtacgacccg cccaactacg
     3421 tgaagaatta cgcggaaacc cggcatctgc catttcccgt ggccatcgat gcgcagggca
     3481 agatcgccaa ggcgttcggc gacattcagc taacgcccac cgcgttcctc atcgacaagc
     3541 agggccatat catcaagcgt tacctgggca attacgacca cgccgcgttc atgcgaacgg
     3601 tggaaaaagc gcttgccagc agttagccgc ccccaactcg gcgcggagcc cggggttcgc
     3661 cccgccccgc gcttccggat tccggccgcc cgcaaggcgg ccgcatgctt tgcgacgcga
     3721 ccgcctgaac ggtagccgcc acgttcaatc cagcttcctc ataccgccat gacccagcaa
     3781 tcccccgaat catccagcta cccgtcaacc gtcaaggcgg acgtcctgtc cgaggcgctg
     3841 ccctacattc gccggttcca cggcaagacc gtcgtcatca agtacggcgg caacgccatg
     3901 accgaagagc aactgcaacg cagtttcgcg cacgatgtcg tgctgctcaa gctggtgggc
     3961 ctgaaccccg tggtcgtcca tggcggcggt ccgcagatca accaggcgct caagcgcatc
     4021 ggcaaggaag gctcgttcgt ccaaggcatg cgtgtcacgg acgccgagac catggaggtg
     4081 gtcgaatggg tgctgggtgg ccaggttcag caggacatcg tcatgatgat caacgaggcg
     4141 ggcggcaagg ccgtcggtct caccggcaaa gatggctgcc tgatccaggc caccaagaaa
     4201 ttgatggctg acgacgccaa ccccggcaag tggctggaca tcggctatgt gggcgacatc
     4261 gcgcgcatcg aaccagcggt ggtcaaggca ctgcaggacg accagttcat acccgtgatt
     4321 tcttccatcg gctatggcga ggacggcatg gcctacaaca tcaacgccga cgtcgtggcc
     4381 ggcaagatgg ccgaaaccct gggcgccgaa aagttgatca tgatgaccaa caccccgggc
     4441 gtgctcgaca agaatggcca gcttctgcgc aacctgtcgg cgcaaaccat cgacgaactg
     4501 ttcaaggacg gcacgatttc aggcggcatg ctgcccaaga tctcgtcggc gctcgatgcc
     4561 gcgcgcaatg gggtcaactc cgtgcatgtg gtggatgggc gcgtcccgca ttgcctgctt
     4621 ctggaaatcc tcacggaccg tggcgtcggc accatgatca gctcacactg agggcacgag
     4681 agcgatgatg tacccgggaa gaccgcaggc ccgccgcacc cggccgacgc gttcgtcgca
     4741 ggcggccaat accgagcccg tctggctctt cgacctcgac aatactctgc acgattcgtc
     4801 cagggagatt ttcaaggcca tcgatcgctc catgacggcc gcggtggcca gcagcctgga
     4861 catcaccgtc gaagcggcca acctcttgcg ggcgacgtac tggaaacgct acggggcgac
     4921 cgtcatcggc atggtgcgcc accatgacat cgacgccagc gcgttcctgc gcttgagcca
     4981 tcaattcgat gtcgcctcga tggtccatgc cgaaaccggc atggcgtaca agctcgggcg
     5041 gttgcccggg cgcaagtatg tattcacgaa cgctccgctg ggctatgcgc gcacggtgct
     5101 gaagacgctc aagatactgc aatgcttcga cggcgtctgg gccatcgacg acatggtgct
     5161 gcaaggcagg ttccgcccca agccgtctgc agccctgatg cgccaagcga tcgcccgatt
     5221 aggcgttcca gcgcaccgcg tcatactggt ggaagacaca ctgaggaatc tcaaaagcgc
     5281 ccgtcaagcc ggcatgcgga cggtttacgt ctaccatccg ggcaccccat tctcgcgtgg
     5341 cgcgcatggc cgcgaccagt acgtggatgt gcgcgtcaac tcgatcggtc atttactgat
     5401 tggccggcgc gccccggctg aatcgcgccc ctccccaacg ccatgatccg tcccgccatg
     5461 cctgccagcc ggagggcgca gatcctcgac accttgtccg ccatgctggg ccaggccgat
     5521 gccggccgtg tgaccaccgc ggcgctcgcg gctcgtctcg cggtatccga gggggccttg
     5581 taccggcact tcagcggcaa ggcggaaatc ttcgaagccc tcatcgatgc ggccgaggcc
     5641 ttgattgaag aggatctgga tcatatcgcc accactgaac ccgatgcacg actgcgtcta
     5701 cgtaaaaacc tgcggacact gcggctcttc gccgaacgac acccagggct ggcgcgcgtt
     5761 ctgaccggcc atgcactatc tggagagggc ctcgacctga cccaacgcgc tgatgccgtc
     5821 atcgaccacc tgcggagtcg actcgcacag gatgccgcaa aggcgggccc ggccttcgac
     5881 gtccaccctg acgccacggc gcgggttctc acgcagtgga ttctgggttg ctggcaaaga
     5941 ccggcgcggc gaaacccgcc ccgaaccgat acctcactcg acgacgaact ggcgttcctg
     6001 gggttgtaac gcgccgcgat tcagcgatgc gagcaaaccg ggcagtcggg atcgcgctcg
     6061 aatcggattt cgtgccattg cgcggacagg gcgtcgaaac attgcagacg ctgcgtcgcc
     6121 ggctcaccca ccccggtcag cagcttgatc gcctcgatcg cctgcatgct gccgatcact
     6181 cccaccagcg gcgccaatac gcccgtcgtg gcgcaattgg cctcgtgagc cccaccggcc
     6241 tcggggaaca agcagtgata acacggggag tcggcgtggc gcagatcgaa cacggcgacc
     6301 tgcccggaaa aacggatggc cgcccccgac accagcggct tcccatgcgt ggcgcaggcc
     6361 cggttcaccg cgtgccgggt cgcgaaatta tcacagcaat cgagcaccag atcgacgccg
     6421 gagaccactc tggagagatc cgcaccctgc aggcgttgtt ccagagcttc gacatgaacc
     6481 tcgggattga gatcggccag catatgacgg cccgaacgcg ctttgggcca ccccacgcgc
     6541 gcggtggtgt gcagcaactg acgctgaaga ttgcccagat cgacgatgtc atcgtcggcg
     6601 agcacgatgt ggcccacgcc cgaaccggcc ag
//
LOCUS       DASTNK010000103        16657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_72385, whole genome shotgun sequence.
ACCESSION   DASTNK010000103 DASTNK010000000
VERSION     DASTNK010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 16657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16657
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72385"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VFR20_07725"
     CDS             complement(<1..483)
                     /locus_tag="VFR20_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU0230445.1"
                     /translation="MPHESRLSRELRILLQSRRIAALGTTDTDGNPFVSMVPFAACPA
                     LNRIVIHVSGLAAHTRNLQDRPVVSLLVTEGETPNEPVHALQRLTIQGRASVLEQGDD
                     EWRACRSAYLSRFPEAESMTQLNDFRFVAIEADHGRHVAGFGTARDVTPEEIARILKP
                     A"
     gene            complement(565..1803)
                     /locus_tag="VFR20_07730"
     CDS             complement(565..1803)
                     /locus_tag="VFR20_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0230446.1"
                     /translation="MKRIIIVGAGFAGLWSALGAARELDEQGIPDKDVEILVINPTDY
                     HSIRVRNYEEPLEQSLVPLSEVLVPAGVAWMQGAVIDIDPAGQRVLVNTDESARHLDY
                     DRLVLAAGSKLAHPPIPGLVEFAFDVDTYDGAKQLGDHLRSLPARPEAPGRYTAVVVG
                     AGLTGVEIAAELPSRLAAIVGDAARDDIRVILADRTPKISQGMGGAQPVIERAMRELG
                     VELMPNFSLSAVDDRGIDLADGTRIDAATVIWCGGMHANPLAGKLNVPLDALGRLPVD
                     EFLRVKDVPGVFAAGDCARLLIDGERPSVMSCQHGRPMGRYAGHNVVADLLGLDMLPL
                     RIDWYTTILDLGPWGAVYTEGWDRHLVAEGAPAKQTKQLINGQRIYPPRRIRRDILQA
                     AAPVVQAPPPNYATIADQKI"
     gene            2008..2319
                     /locus_tag="VFR20_07735"
     CDS             2008..2319
                     /locus_tag="VFR20_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HEU0230447.1"
                     /translation="MKSTRRTFLIASLGAASSLALARQAVAAPEKVSESDPLAVSMGY
                     HIDASKIDKAKEPKYQTGEDCGNCQLFQGKAGEDWGPCPIFGGKLVHHDGWCKAYTKK
                     A"
     gene            complement(2502..3434)
                     /locus_tag="VFR20_07740"
     CDS             complement(2502..3434)
                     /locus_tag="VFR20_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU0230448.1"
                     /translation="MKHRVIFTTLILSTFFWGANFNLGKSVVQATDPLTAAAWRFMLA
                     GAAMTVYMLFTEGVNWRGLRQNAVALVAMAAIGIFGFNISFFFGLQTTSSVNGSLIMT
                     LNPTITVILTALVISEAISWKQMLGLALSFCGVVTVVTGGNPAALLRFHFAIGDALIL
                     LGNLCWAAYSVIGKKWVRNLSAVQTTTSTMLIGALAMVAIALFQHGGSLPVPSTHTFG
                     AIAIMALFGTVLAYLWWNNGIRTIGPARTAVFFDLVPIFTMLIAISLGEQVSPAQYVG
                     AVLVISGVLFSSGALDMWLSRARPTGQPCQTPAR"
     gene            3611..4495
                     /locus_tag="VFR20_07745"
     CDS             3611..4495
                     /locus_tag="VFR20_07745"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0230449.1"
                     /translation="MDWDDLRYFLAVARSAGLTPAAQALGVSPATVSRRIDAFEQAMG
                     MTLFLRRQTGYTLTDDGETLLAAALPVEQAMLGFARRSQDAGRPGTWAGSIRVATSDT
                     IATHWITPMLPLFVQGHPGLQVELVTGVNTVNLSRRDADIALRMSPPTREEEGEYIAL
                     RAGAMAFAAYITVDDADAQRDWRTLPYVNWGESLAHVPMARWAASMFPGRQPVLLSNS
                     MPVMIAAAEAGLGVTVLPESIGERMAALVRVDFACARDLWLVYHRDLRHNPRVVAMKD
                     FLLGVVRHYEGRPAHAVV"
     gene            complement(4531..5091)
                     /locus_tag="VFR20_07750"
     CDS             complement(4531..5091)
                     /locus_tag="VFR20_07750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004528179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0230450.1"
                     /translation="MNAISSQYTTTALVRDATPADIAAITEIYTPYVLQGLATFEEKP
                     PSEADMRARREAVLALGLPYLVAELDGQVAGYAYATHYRPRSAYRYTIEDSIYVRHGL
                     HGHGIGTSLLRVLIERCEKGPWRQMLAVIGDSANVGSIALHQRFGFVSGGTQRAVGFK
                     LGRWIDTVQMQRPLGAGDTTPPDAMP"
     gene            complement(5222..5839)
                     /locus_tag="VFR20_07755"
     CDS             complement(5222..5839)
                     /locus_tag="VFR20_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEU0230451.1"
                     /translation="MTTYETVLQSLEQLGWAVSDGLAPAGLAPRLYDECRALWEAGRF
                     RPAEVGRGPRQARQDAIRGDAIHWLEPDSAGPASREFLVWITQLRTMLNQAFFMGLNT
                     EEFHFARYPAGAGYTRHLDQHQGATARKVSVTLYLSPDWPVDGGGELRLYEEKAVSGP
                     VATVLPTQGRMVLFLSDQVPHDVQPGTQPRWSLTGWLRTAAGLPL"
     gene            5941..6138
                     /locus_tag="VFR20_07760"
     CDS             5941..6138
                     /locus_tag="VFR20_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230452.1"
                     /translation="MARVASDFNQESLLPVEFGFLLAPKFSMLALAGVLEPLRMAGQC
                     IHPEVCESRASQIGLSERCMS"
     gene            6330..6935
                     /gene="ddpX"
                     /locus_tag="VFR20_07765"
     CDS             6330..6935
                     /gene="ddpX"
                     /locus_tag="VFR20_07765"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HEU0230453.1"
                     /translation="MAVGNALQKDAAPALDDRPGQLVEITPDTHPVVIDLVYATPHNL
                     AGRPVYASDARCALRPEAAACLLRAATAARRAGYTLKVFDAYRPAAAQAIFWAECPDP
                     RYVSDARKGSNHTRGTAVDVTLLDEQGKELDMGTGFDAMRDQSHHDRDDLPPTVQRHR
                     MQLLGIMLRAGFRSINTEWWHYELPDSHSYALLDDPIVRVV"
     gene            7225..8808
                     /locus_tag="VFR20_07770"
     CDS             7225..8808
                     /locus_tag="VFR20_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0230454.1"
                     /translation="MYEFANRRATALALGRHALAALVAGLGLVALEPSVFAMAPKDTL
                     VIGKPADPQTLDPAITIDNNDWTVTYPSYQRLMRYKQVNGKGSTEVEGDLAASWKASP
                     DNLTWTFKLRHGQKFADGAPVDANAVKFSFDRLMKLQQGPSEAFPPHLQVTVVDPMTV
                     QFKMDKPFAPFLYTLANDGASIVNPDVAKRATDIKAYLSGHTAGSGPYQLVSWQKGQS
                     LTLEPNPHYGGSHKPHLAKVVVKIVPEASSRRLQLEKGDLDIAEELPVDQLAALKKAP
                     GVKVAEFPSLRVTYLYLNNKKGPLSNVHLRRAVVDAMDYKGIIDGIMLKQAKQTRGPI
                     PEGMWGYDAKLPLPTQNLTKAKAELAKSGESHPTLVLRYSTQDPNWEPIALTTQANLA
                     AIGIKVKLEKLANATLRENLDKGNFDISIGNWSPDFADPYMFTSYWFDSSKGGLAGNR
                     SFYSNPQVDKWLREAATLSDQAKRTALYDKVQEQVTKDAAYVYLFQKNYQVGMRNDVK
                     GFVFNPMLERVFNVADMTK"
     gene            8881..9897
                     /locus_tag="VFR20_07775"
     CDS             8881..9897
                     /locus_tag="VFR20_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0230455.1"
                     /translation="MSWLPIVKKRLFSLILVVIGVSLITFVISHLIPGDPARLIAGER
                     ATPAIVAHIRHQLGLDLPLYEQYWRYIVQLAHGDLGMSIRTHRPVLQDIRAFFPATLE
                     LAVVALILATLIGIPLGVLSAVYRNKTVDQIVRTISVTGISTPAFWLGLGLIVVFYGH
                     LGWFPGGGRLDQGMTPPTDITGFYLVDSLLTWNIPVFLNALKHIVLPAFTLGFVHLGI
                     VARQIRSAMLEQLGEDYIRTARAFGLSRLTVVVRHALPNALIPSVTVLGLALGDLLYG
                     AVLTETVFGWPGMGAYVVESIQALDFPAVMGFAVVVSFVYVLLNLVVDLLYRWIDPRI
                     TEVN"
     gene            9897..10778
                     /gene="ddpC"
                     /locus_tag="VFR20_07780"
     CDS             9897..10778
                     /gene="ddpC"
                     /locus_tag="VFR20_07780"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF007376.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HEU0230456.1"
                     /translation="MASVPVVAAQGSWKDALSRFAYQLKKSPMTMLGLGIVVVVIVAV
                     SCANLLAPYNPDMISLVHRLAPPSAQHWFGTDEVGRDIFSRVLYGGRQSVGVGLFVAF
                     ASSIVGAIIGCFSGVLGGWIDNVIMRAMDVVLSVPSLVLTMALAAALGPSLFNAMLAI
                     TIVRIPFYVRLARGQTLGIREMAYVKAAHTFGATRRHIVSWHVVRNALSPIIVQTTLD
                     IGNAILMAAALGFIGLGAQQPTAEWGAMVSTGRNYLLDQWWYTVFPGFAILITAMGFN
                     LLGDGVRDMLDPRLKAK"
     gene            10865..11890
                     /locus_tag="VFR20_07785"
     CDS             10865..11890
                     /locus_tag="VFR20_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0230457.1"
                     /translation="MRGVMDARRGLAPASGGAVLEIHDLSLEFPVYKGSVKALNGVSL
                     TVAPGEIVGVVGESGSGKSVTAMAALRLLPPDGFKITGGSLRLLGRDILAASEKEMRA
                     VRGGDAAMIFQEPMTALNPTRRVGAQLVDVIRCHQSLSREQALVKAVGLLRDMYIADP
                     EQILRRYPFELSGGMRQRVMIALAFSCDPALLIADEPTTALDVTVQRQVLLLLREKAR
                     ERGTAILFITHDMAVVSQFCDRVYVMYAGSVVEEGATARVMARPRHPYTLGLLAGLPE
                     NASPGAMLPSIRGQVPNMAAPPAGCAFRDRCDHADEACLAHPALRDLGEPGRKAACWH
                     VLNGEPA"
     gene            11857..12879
                     /locus_tag="VFR20_07790"
     CDS             11857..12879
                     /locus_tag="VFR20_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0230458.1"
                     /translation="MLACSERGTRMMREAEQAPVLALEDVEVRFPVAADWLGRPNRYA
                     HAVNGVSLDVRAGEILGIVGESGCGKSTLAQLLMGLLEPSGGSVRRPGASTGKANVQI
                     VFQDPQSSLDPRLPVWRVMIEPIYVRDGGRLSALRARAVELAGQVGLRAEQLDRYVHE
                     FSGGQRQRIAIARALSSDPDVIVLDEPTSALDISVQAQILNLLLSLQRAHNLTYVLIS
                     HNVSVVRHLCHRVAVMYLGQIVEIGSARDVLGDPRHPYTRVLLDAVPRLGEPFADGSP
                     TVNTELPGNRVLPTGCFFRDRCPLATAGCEKPQRLVPLPGPASRSSRCHLAPMARRAW
                     RAPAER"
     gene            13011..14207
                     /locus_tag="VFR20_07795"
     CDS             13011..14207
                     /locus_tag="VFR20_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU0230459.1"
                     /translation="MTSSPPSPLFSQRARKLTGSAIREILKITQRPDIISFAGGLPAP
                     QGFPVEELHTAFDRVLSSSAQVGLQYGPTEGYQPLREWVAQDMVARGAEVSPEEVLIV
                     SGSQQALDLLGKLFIDPGSKILIEAPSYLGAIQSFSLFEPEYRAIPTDGGGLIPDGLT
                     DDCVRGARFVYALPNFQNPTGLTLSLERRRALVARCAAAGLPIVEDDPYGDLRYAGVA
                     QPSLLNLGREAGATVIRMGTFSKVLSPGLRLGYIVAPRPLIAKLAQIKQATDLHTAML
                     TQMAVYETVKNGFLARHLPGIRELYKNQCGHMLDAMARDFPDSATWTRPEGGMFIWVT
                     LAPHLDAGQLLQTAVERKVAFVPGAPFFAGVVPQLNTLRLSFVTVPEPKIREGIATLG
                     RLLKEA"
     gene            14240..15193
                     /gene="ubiA"
                     /locus_tag="VFR20_07800"
     CDS             14240..15193
                     /gene="ubiA"
                     /locus_tag="VFR20_07800"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEU0230460.1"
                     /translation="MTSASNSPSSSHQLDASIPDLSDMRPDDWVEHWLPRSWGPYARL
                     CRLDRPVGTWLTLLPCLAAVVQAADGWPALFRLFIFSLGALLMRGVGCTINDIWDRDF
                     DLKVERTRFRPLTSGQVTLRAAVVFLFAQLAVCALLLLTINAYSRWLAVALVPIVIIY
                     PLCKRFTYWPQVVLGIGFNWGMLMAWSDTQNVVPLAAVAMWVGAVLWQVGYDSIYAYV
                     DVRDDRMLGLHSTAMRFADRGKRWITGFYVATVVLWLYAGIALNMGWGYFIVLAVITG
                     HFIWQMRLFSLERPDRNFMLFRANIWVGVLLLVAALAGTVI"
     gene            15395..>16657
                     /locus_tag="VFR20_07805"
     CDS             15395..>16657
                     /locus_tag="VFR20_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0230461.1"
                     /translation="MADQGPEQADLEVDYIIVGAGSAGCLLANRLSADPSRKVLLLEA
                     GGRDDWRWIHIPVGYLYCIGNPRTDWCYRTRPDPGINNRVLGYPRGRVLGGSSSINGM
                     IYMRGQAADYDGWAALGNTGWSWSDVLPVFKQVEDHYAGESEFHGASGTWRVEQQRLS
                     WPILDAFREAAAQAGIRKVTDFNRGDNEGCGYFEVNQRQGVRWTSAKAFLHPVANRAN
                     LTVLTDAQAQGLVLSGRRAEGVRFTHGGQSKFARARAEVILSTGSIGSAQLLQLSGIG
                     HGPLLQSLGIPVVHDLKGVGENLQDHLQLRLIYRVQGAKTLNTMANSLWGKAVMGLQY
                     VFQKRGPLTMAPSQMGAFARSDAQRSRANLEYHVQPLSLEKFGDTLHDFPAFTASVCN
                     LQPSSRGHVHITSSNPQEAPAILCNYLQT"
BASE COUNT         2859 a         5059 c         5509 g         3230 t
ORIGIN      
        1 cgccggtttc aggatgcgtg cgatttcctc tggggtgacg tcgcgggcgg tgccgaagcc
       61 ggccacatgg cgtccgtggt cggcttcgat ggccacaaag cggaagtcgt tcaactgtgt
      121 catggattca gcctctggga aacggctcag gtacgccgac cggcacgctc gccattcgtc
      181 gtcgccttgt tccagaacgc tggccctgcc ctggatggtg aggcgctgca gggcatgcac
      241 gggttcattc ggcgtctcgc cctcggtcac aagcagcgac acgacgggcc gatcctgaag
      301 attgcgggtg tgggccgcca agccgctcac gtggatcacg atccgattca gcgctgggca
      361 tgccgcgaac ggcaccatgg agacgaacgg attgccgtcc gtgtcggtgg tgcccagcgc
      421 ggcgatgcgc cgggattgaa gcaatatgcg aagctcgcgg gagagtcggg attcatgcgg
      481 catggctgcg atgtccttgg aaaggtgtag cgcgtcacct ttgccccggt gggtggtgtc
      541 tgtgcggacg caaataagaa ctattcatat tttctggtcg gcaatggtgg cgtaattggg
      601 cggcggtgcc tggacgacgg gggcggccgc ctggagaatg tcccggcgga tacgccgggg
      661 cgggtagatg cgttggccgt tgatcaattg tttggtctgc ttggccgggg ccccctcggc
      721 aaccagatgc cgatcccagc cttcggtata cacagcgccc cagggaccga ggtcgaggat
      781 ggtggtgtac caatcgatcc gcagcggcag catgtccagg cctagcaggt cggccacgac
      841 gttgtggccg gcgtagcggc ccatggggcg gccgtgttgg cacgacatga cggacgggcg
      901 ttcgccgtcg atcagcaggc gggcgcagtc gccggcggcg aacacgccag gcacgtcttt
      961 cacgcggagg aactcgtcga ctggtagccg gcccagcgcg tcgagcggga cgttgagctt
     1021 gcccgccaga ggattggcgt gcatgccacc gcaccagatg accgtggctg cgtcgatacg
     1081 ggtgccatcg gccagatcga tcccccgatc atccaccgcg ctcagtgaga agttcggcat
     1141 caattcgacg ccaagctcgc gcatggcacg ttcgatcacg ggttgcgcgc cccccattcc
     1201 ctggctgatc ttcggggtgc ggtcagcgag aatcacgcgg atatcgtcgc gggccgcatc
     1261 gcccacgatg gccgccaggc gcgaggggag ttcggccgcg atttcgacgc cggtgagtcc
     1321 ggcccccacc accaccgcgg tgtagcgacc cggcgcctcg gggcgcgcgg gcagtgatcg
     1381 cagatgatca cccagctgtt tcgcgccgtc gtaggtgtcg acgtcgaatg cgaattcgac
     1441 caggccgggt atgggcggat gcgccagctt gctgcccgcg gcgagcacca gccggtcata
     1501 gtccagatgc cgcgcgcttt cgtcggtatt cacgagcaca cgctgccccg ccggatcgat
     1561 gtcgatgacg gcgccttgca tccaggcaac gccggcgggc acgagaacct cggacagggg
     1621 gactagggat tgctccagcg gctcttcgta attacgtacc ctgatgctgt ggtaatcggt
     1681 cgggttgatg accaggatct cgacatcctt gtccgggata ccctgttcgt caagctcccg
     1741 tgccgcgccc agtgcgctcc agaggcccgc gaaaccggca cccacaatga tgattcgctt
     1801 catgatgtgc tccaaggaga tcgtttcgat tctagtcctt cgaccgcgtt ccccatgtat
     1861 gaattccctg gttcgtcgtg cgggcatgta cgccccgtgt tggctctaag ggtggcgtaa
     1921 ggaaagcggc gctactctac aaagtgcgcc ataaagtgcc tcgatcgtgg cgccatttat
     1981 tcgatgtgta gaaaaaagga gccagcaatg aagtcaaccc gaagaacatt cctgatcgcc
     2041 agccttgggg cggcgtcgtc cctcgccctg gcgcgccagg cggtggcagc gccagagaaa
     2101 gtcagcgaga gcgatccgtt ggccgtatcc atggggtacc acatcgacgc cagcaagatc
     2161 gacaaggcca aagaaccgaa ataccagact ggcgaagact gtggcaattg ccaactcttc
     2221 caggggaaag cgggtgaaga ctggggcccc tgtccgatct tcggtggcaa gctggtccat
     2281 cacgacggct ggtgcaaggc ctatactaag aaggcgtaag ccaatattgg cgccgtgctt
     2341 gtgcgcaggc gccgggttcg agtattcgaa tcaaccaagc aagacgcttg tgagacacgc
     2401 ccccgggacg ggacgcaaga cgagaagcct cgcggcagct gctgccgtga ggctttttcc
     2461 tgtctgtcga cgatgtgtgc cgatgcgcgc acggggtagg gttaccgcgc tggcgtctgg
     2521 caaggctgtc cggtggggcg cgcacggctg agccacatat ccagcgcgcc ggaagaaaac
     2581 aacacgcccg agatcaccag cacggcgccc acgtattggg ccggcgacac ttgttcgccc
     2641 agcgagatgg cgatcagcat ggtgaagatg ggcaccaggt cgaagaacac cgcggtgcgg
     2701 gccgggccga tggtgcggat gccgttgttc caccacaaat acgcgagcac cgtgccgaac
     2761 agggccatga tggcgatggc gccgaaggtg tgtgtcgaag gcacgggcag cgaaccgcca
     2821 tgctggaaca gggcgatggc gaccatggcc agcgcgccga tgagcatggt gctggttgtc
     2881 gtctggaccg cggaaaggtt cctgacccat ttcttgccga tcacgctgta agccgcccag
     2941 cacagattgc cgagcagtat cagcgcatca ccgatggcga aatggaagcg caacagtgcg
     3001 gccgggttgc cacccgtcac gacggtgacc acgccgcaaa agctcagggc caggcccagc
     3061 atttgtttcc aggagatggc ttcgctgatg accagtgccg tgaggatcac ggtgatggtg
     3121 gggttgagcg tcatgatcag cgagccgttg accgaagagg tcgtttgcag cccgaagaaa
     3181 aacgagatgt tgaagccgaa tatcccgatg gccgccatgg cgaccagggc cacggcgttc
     3241 tggcgcaggc cccgccagtt aacaccctct gtgaacagca tgtacaccgt catggcggcg
     3301 cccgccagca tgaaccgcca ggcggcggcg gtgagcgggt cggtggcctg cacgacggac
     3361 ttgcccagat tgaaattggc accccagaaa aaggtcgaga gaatcagcgt tgtgaagatg
     3421 acccggtgtt tcatttcggt ttccatgaag aatgtgtctg ggcgtattct gaattttcta
     3481 gattgaaaaa tatatggaat aattgtcaaa atcatttttc aattttgaaa aataccgaat
     3541 tgagtggcag gcctggcgtg acgttcacct tatgggcttc gcatctgcgc caccccggag
     3601 ggctttaggc atggattggg acgatcttcg ctatttcctc gctgtggcgc gctccgcggg
     3661 gctgacgcca gcggctcagg cgctgggtgt cagcccggcc actgtgtcgc gacgcatcga
     3721 cgcgttcgag caggccatgg gcatgacgct gttcttgcgc cggcagactg gctacacgtt
     3781 gacggacgac ggcgaaaccc tgctggcggc ggcgttgcct gtggagcagg cgatgctggg
     3841 ctttgcgcgt cgttctcagg acgcaggccg gccgggcaca tgggcgggaa gcatccgcgt
     3901 ggccacgtcg gacaccatcg cgacgcattg gatcacacca atgttgcccc tgttcgtgca
     3961 aggccaccct ggtctgcagg tggagcttgt caccggggtc aacaccgtga atctgtcgcg
     4021 tcgcgatgcc gacatcgcgt tgcgcatgtc tccaccgaca cgggaggagg aaggggaata
     4081 tattgccctg cgcgcgggcg cgatggcgtt tgccgcatac atcaccgttg atgatgcgga
     4141 tgcgcagcgt gattggcgca ccctgccgta cgtcaactgg ggcgaatctt tggcgcatgt
     4201 gccaatggcc cgctgggccg ccagtatgtt cccgggcagg caaccagtcc tgctcagcaa
     4261 cagcatgccg gtgatgatcg cggcggccga ggccggtctg ggggtcacgg tgttgccgga
     4321 gtcaattggt gaacggatgg cagcccttgt tcgagtggat ttcgcctgcg cgcgggacct
     4381 atggctggtg tatcaccgcg atcttcgcca caacccgcgg gttgtggcca tgaaggattt
     4441 cctgcttggc gtggtgcgcc actatgaagg gcgtccagcc catgccgtcg tttgaagggc
     4501 aatgggccaa tacattcagt ttaaacaccc ctatggcatg gcgtccggcg gcgtcgtatc
     4561 gcccgccccc agggggcgct gcatttgcac ggtgtctatc cagcggccaa gcttgaagcc
     4621 gacggcgcgt tgcgtgccgc cagacacgaa gccaaagcgt tgatgcaacg cgatggagcc
     4681 aacgttggca ctgtcgccga tgaccgccag catctggcgc cacgggcctt tttcgcagcg
     4741 ttcaatcaac acccgtagca gagacgtgcc gattccgtgt ccgtgcaacc cgtggcgcac
     4801 atagatggaa tcctcgatgg tgtagcgata ggccgagcgc gggcggtagt gcgttgcgta
     4861 ggcatagccg gcgacttgcc catcgagctc ggcgaccaga taaggcaggc ccagcgccaa
     4921 cacggcctcg cggcgggcgc gcatgtccgc ctcggagggc ggcttctctt cgaaggttgc
     4981 caggccttgc agcacatacg gtgtgtagat ttccgtgatg gccgcgatgt cggcgggggt
     5041 ggcgtcgcgt acgagcgctg tggtggtgta ttggctggag atggcgttca tgaggttcat
     5101 ggaaggatat atgtggccgg gagtttgagc cggagtcgct ccggcgccat gcgtgcgcga
     5161 agcgaaatac aaccgttgga attttcgcac caaaacccgc gtcaaaaccg caggccccgc
     5221 atcaaagcgg caggccggcg gcggtgcgca gccaaccggt caggctccag cgcggttgcg
     5281 tgccaggctg cacgtcgtgt ggtacctggt cgctcaggaa cagcaccatg cgtccctggg
     5341 tcggcagcac cgtggcgacg ggcccggaga cggctttctc ctcgtacagc cgcagttcgc
     5401 cgccgccatc gacgggccag tcgggactga ggtacagggt gaccgacacc ttgcgcgcgg
     5461 tggcgccttg atgctgatcc agatgccggg tatagcccgc tccggcaggg tagcgcgcaa
     5521 agtggaattc ctcggtgttc aggcccatga agaaggcctg gttcagcatc gtccgcagtt
     5581 gggtgatcca gaccagaaac tcccggctgg cagggccggc ggaatcgggt tccagccaat
     5641 gaatggcgtc acctcgtatc gcgtcctgac gggcctggcg ggggccgcgc ccgacttccg
     5701 cagggcggaa tcgccccgct tcccatagtg cgcggcattc gtcatacagg cgcggagcca
     5761 gtcctgcggg cgccaagccg tcggacacgg cccaaccaag ctgctccagg gattggagca
     5821 cggtttcgta agtagtcatg gagccacgcg cagcagatag aatgttgctg gaacctggca
     5881 tcatgagaag atcttctggc ccatgacgcc caggggaaac ctgaatagga gcacggagag
     5941 atggcgcgtg tcgcgtcgga ttttaatcaa gaatcgcttc ttccggtgga gttcggattc
     6001 ctgctggcgc caaaattttc gatgcttgcg ctggccggtg tgctcgagcc actgcgcatg
     6061 gctggacaat gcatccaccc agaggtgtgc gaatcccggg ccagccagat cggcctgtcc
     6121 gaacgttgca tgagttaggc ggaaattgga ccgcccaatg ttatggttcg tgaaatgcca
     6181 actccccctg tgccgcagag ccggaaggga ggattgctag tagaattttg aggaatgtcc
     6241 aggcggcgcg cgccaattgt ctgtcgcgcc tgtacgcata tcgggctggc gaggcccata
     6301 tccaaatctc gattcccgga ggtacgcagg tggcagttgg taatgcattg cagaaagacg
     6361 ccgcaccggc gctcgatgat cgccctggcc agttggtcga gatcacccct gatacacacc
     6421 cggtcgtcat cgacctcgtc tacgccacac cccacaacct cgcgggccgg cccgtgtacg
     6481 cttccgatgc gcggtgcgcg ctgcgacccg aggcggcggc ctgtctgttg cgtgccgcca
     6541 cggccgcgcg tcgggccggc tataccctca aagtcttcga tgcttaccgt cccgcagccg
     6601 cccaggcgat tttctgggcg gagtgcccgg atccgcgcta tgtgtccgac gcgcgaaaag
     6661 gctccaacca cacacgcggg acggcggtcg acgtcactct gctcgatgag cagggcaagg
     6721 aactggacat gggaaccggc ttcgatgcca tgcgggatca gtcccatcac gaccgcgacg
     6781 acctcccgcc cacggtgcag cgtcatcgca tgcagttgct gggcatcatg ctgcgcgctg
     6841 gttttcgttc catcaacacg gaatggtggc actacgaact gcccgattcg cattcctatg
     6901 cgttgctgga tgatccgatt gtccgggtgg tgtgacgagc ggatcgtttc caggctgtgg
     6961 ctcttcctga tgctttgccg ccactccata ccgattgggt tggtgcggaa tgtttcgaac
     7021 cctagggtta ttactaggct tgtcgcgttt ccatcgcttg taagctacaa agctttcgga
     7081 ttgagacgct gtctggcgta tttcgcgtgg cgtgtcgcgc ctcgacgtgg cccgaatctc
     7141 gtcaattcct gtaatcgttt tctgtttatc gccgcgacgt gctgtgttga cccgcgacgt
     7201 tcccctttcc cctggaggta atccatgtac gagtttgcga atcgtcgcgc cacggcattg
     7261 gcgctgggca ggcatgcatt ggccgcgttg gtggcggggc tggggctggt ggcgctggag
     7321 ccgtccgtgt ttgccatggc gcccaaggac acgctggtca tcggcaagcc cgccgacccg
     7381 cagacgctcg atcccgccat caccatcgac aacaacgact ggacggtgac ctacccgtcc
     7441 tatcaacggc tgatgcgcta caagcaggtg aatggcaaag ggtccaccga ggtcgaaggc
     7501 gacctggcgg caagctggaa ggcctcgccc gacaatctca cctggacttt caagctgcgg
     7561 cacggccaga agttcgccga tggcgcgccg gtggacgcca acgccgtgaa gttctcgttc
     7621 gatcggctca tgaaattgca gcaaggccca tccgaggcct tcccgccgca tctgcaggtc
     7681 acggtggtcg atccgatgac ggtgcagttc aagatggaca agccgttcgc gccgttcctc
     7741 tataccctgg cgaacgacgg ggcgagcatc gtcaaccccg acgtggccaa acgggcgacg
     7801 gacatcaagg cgtatctgtc cggccatacg gctggctccg gcccgtatca actggtgtcc
     7861 tggcagaaag gccagtcgct cacgctggaa cccaatcccc attacggcgg cagccacaag
     7921 ccccatctgg cgaaggtcgt ggtcaagatc gtgcccgagg cctcgtcgcg gcgcctgcaa
     7981 ctggaaaaag gcgatctgga catcgccgag gaactgccgg ttgaccagct cgccgcgctg
     8041 aagaaggcgc ccggggtgaa ggtggcggaa ttcccgtcgc tgcgcgtcac ctatctgtac
     8101 ctgaacaaca agaaggggcc gttgtccaac gtgcatctgc gccgcgccgt ggtcgatgcc
     8161 atggattaca aaggcatcat cgatggcatc atgctcaagc aggccaagca gacacgcggc
     8221 cccatcccgg aaggcatgtg ggggtatgac gccaagctgc cgctgccgac ccagaacctg
     8281 acgaaggcca aggccgaatt ggccaagtcc ggcgaaagcc accccacgct ggtgctgcgc
     8341 tattccacgc aagaccccaa ctgggagccc atcgcgctga cgacccaggc caacctggcg
     8401 gccataggca tcaaggtcaa gctggaaaaa ctggccaacg ccaccctgcg tgagaacctg
     8461 gacaagggta atttcgacat ttccatcggc aactggagtc cggacttcgc cgatccgtac
     8521 atgttcacca gctactggtt cgactcgtcc aagggcgggc tggccggcaa ccgctcgttc
     8581 tattccaatc cgcaggtcga caagtggctg cgcgaggcgg ccaccctcag cgaccaggcc
     8641 aagcgcaccg cgctctacga caaggtgcag gaacaggtca cgaaagacgc tgcctacgtg
     8701 tatctgttcc agaagaacta tcaggtcggc atgcgcaacg acgtgaaggg tttcgtgttc
     8761 aatcccatgc tggaacgcgt gttcaatgtg gccgacatga cgaagtaacg tagtcgtggt
     8821 cgggcgtgca tcgggccgag gttcgatgca cgccttctga agtaagtttc tcgaggtttt
     8881 atgtcctggt tgccgatcgt caagaaacgg ctgttcagtc tgattctggt ggtcattggc
     8941 gtttcgctca tcacgttcgt catttcccat ctcattcccg gcgaccccgc gcgcctcatc
     9001 gccggggagc gcgccacacc cgccatcgtg gcgcacattc gtcatcaact gggcctggac
     9061 ctgccgctct acgagcagta ctggcgttac atcgtccagt tggcgcatgg tgacctgggc
     9121 atgtcgatac gcacgcaccg cccggtgttg caggacatcc gggccttctt tcccgccacg
     9181 ctcgagctgg cggtggtggc gctgatcctg gccacgctca ttggcattcc cctgggtgtg
     9241 ttgtcggcgg tctaccgcaa caagacggtc gaccagatcg tgcgcacgat ctccgtcacg
     9301 ggcatctcca ccccggcgtt ctggctggga ttggggctga tcgtggtgtt ctatgggcat
     9361 ctgggttggt ttccgggcgg gggccgtctg gaccagggca tgaccccgcc caccgacatc
     9421 accgggttct atctggtcga ctcgttgctg acctggaaca ttccggtgtt cctcaacgcg
     9481 ctcaagcaca tcgtgctgcc ggcgttcacg ctggggttcg tgcatctggg aattgtcgcg
     9541 cggcagatcc gttcggccat gctcgagcaa ttgggcgagg actacattcg caccgcgcgc
     9601 gccttcggcc tgtcgcgcct gacggtggtg gtgcggcatg ccttgccaaa cgcgttgata
     9661 ccatccgtca cggtgctggg tctggcgctg ggtgatctgc tctatggggc ggtgttgacc
     9721 gagaccgtgt tcggctggcc gggcatgggc gcgtacgtgg tcgagtccat ccaggcgctg
     9781 gattttccgg cggtaatggg attcgccgtc gtggtgtcgt tcgtgtatgt gttgctgaat
     9841 ctggtggttg atctgctgta tcggtggatc gatccgcgca tcacggaggt gaactgatgg
     9901 ccagcgtccc cgtggttgcc gcgcaagggt cctggaagga cgccctgtcg cgcttcgcgt
     9961 atcaattgaa gaaaagcccg atgaccatgc tgggtctggg catcgtcgtg gtggtgatcg
    10021 tcgcggtgtc gtgcgccaat ctgctggcgc cgtacaaccc cgacatgatc agcctggtgc
    10081 atcgcctggc gccgccttcg gcgcagcact ggttcggcac cgacgaagtg gggcgcgata
    10141 ttttcagccg cgtcctgtac ggggggcgcc agtcggtggg tgtcgggctg ttcgtggcgt
    10201 tcgcctcgag catcgtgggc gccatcatcg gctgtttctc gggtgtgctg ggcgggtgga
    10261 tcgacaacgt catcatgcgc gccatggacg tcgtgctgtc ggtgccgtcg ctggtgctga
    10321 ccatggcgct ggccgccgca ttggggccga gcctgttcaa cgccatgctg gccatcacca
    10381 ttgtgcgcat tccgttctac gtgcgcctgg cgcgcgggca gacgctgggt atccgcgaga
    10441 tggcctacgt gaaagccgcc cataccttcg gcgccacgcg ccgccacatc gtgtcctggc
    10501 acgttgtgcg caacgcgttg tcgcccatca tcgtgcagac cacgctggac atcggcaacg
    10561 ccatcctgat ggccgccgcg ctgggcttca tcggcctggg ggcgcaacag cccacggccg
    10621 agtggggcgc catggtgtcg acagggcgca attacctgct ggaccagtgg tggtacaccg
    10681 tgttcccggg gttcgcgatc ctgatcaccg ccatgggttt caatctgctg ggcgatggcg
    10741 tgcgtgacat gctcgacccc aggctgaaag cgaaatagga aggtggcatg acgggaagtg
    10801 ggatgggaac aagcaagcga agcgcgcccc ggcatggcgg ggccccgctc tcgggagggg
    10861 tggcatgcgc ggtgtgatgg atgcccggcg cggactcgcc ccggcgtcag gtggcgcggt
    10921 gctcgaaatc catgatctga gtctggagtt tccggtctac aaagggtcgg tcaaggcgct
    10981 caatggcgtg tcgctcaccg tggcgccggg cgagatcgtc ggtgtggtgg gggaatccgg
    11041 ctccggcaag tccgtgacgg cgatggcggc gctgcgcctg ttgccgccgg acgggttcaa
    11101 gatcaccggc gggtcgctgc gcctgttggg ccgagacatt ctcgccgcct cggaaaaaga
    11161 gatgcgtgct gtgcgtggtg gcgacgccgc catgattttc caggaaccca tgaccgccct
    11221 caacccaacc cggcgcgtcg gcgcgcaact ggtcgacgtg atccgctgcc accagtcgct
    11281 gtcgcgcgaa caggcgctcg tgaaagcggt ggggctgttg cgcgacatgt acatcgccga
    11341 tccggaacag attctgcggc gctatccatt tgaattgtcg ggcggcatgc gtcagcgggt
    11401 gatgatcgcg ctggcgtttt cgtgtgatcc cgccttgctg atcgccgacg aacccaccac
    11461 ggcgttggac gtcaccgtgc agcggcaagt tctgttgctg ctgcgtgaga aggcccgcga
    11521 acgcggcacc gccattctat tcatcacgca cgacatggcc gtggtgtcgc agttctgcga
    11581 ccgggtctac gtcatgtacg cggggtcggt ggtggaggag ggcgcgacgg cgcgggtcat
    11641 ggcccggccg cggcatccgt ataccctggg tctgctggcc ggcctgcccg agaacgcgtc
    11701 gcccggcgcg atgctgccct ccatacgggg gcaggtgccc aacatggcgg cgccgcccgc
    11761 cggctgtgct ttccgggatc gctgcgacca tgccgatgag gcctgtctgg cccacccggc
    11821 gttgcgtgat ctcggggagc caggccggaa ggcggcgtgc tggcatgttc tgaacgggga
    11881 acccgcatga tgcgcgaggc cgagcaggcg ccggtgctgg cgctcgagga tgtggaagtc
    11941 cggttccccg tggcggcgga ctggcttggc cggcccaacc gctacgcgca tgccgtcaat
    12001 ggggtgtcgc tggacgtgcg agcgggcgag attctgggga tcgtgggcga atcggggtgt
    12061 ggcaaaagca cattggccca gttgctgatg gggttgctcg aaccctccgg cggcagtgtg
    12121 cgccggcccg gcgcctcgac aggcaaggcg aatgtgcaga tcgtgttcca ggatccgcag
    12181 tcgtccctgg atccgcgcct gccagtctgg cgggtgatga tcgaacccat ttacgtgcgc
    12241 gatggcggtc gtttgtcggc gttgcgcgcc cgggcagtgg aactggcggg gcaggtgggg
    12301 ctgcgcgccg agcagctcga tcgttacgtg catgagttct cgggcggtca gcgtcagcgc
    12361 atcgccatcg cccgcgcgct gtcgtccgac ccggatgtga tcgtgctgga cgaacccacc
    12421 tcggcgctgg acatctcggt gcaggcccag atcctgaacc ttctgctgtc gctgcagcgt
    12481 gcgcacaacc tgacgtatgt gctgatttcg cacaacgtgt cggtggtgcg gcacctgtgc
    12541 caccgtgtgg cggtgatgta cctgggtcag atcgtggaaa tcggctcggc gcgggacgtg
    12601 ctgggcgatc cacggcatcc ctacacgcgt gtgttgctcg acgccgtgcc gcgcctgggc
    12661 gagccgttcg ccgatggctc gccgaccgtc aacacagaat tgccgggtaa ccgggttctt
    12721 ccgaccgggt gttttttccg ggatcgatgc ccgttggcga ccgcgggctg cgagaagccg
    12781 cagcgactgg tccctctgcc gggccccgcc tcgcgcagta gccgctgcca tctggcgccc
    12841 atggcgagga gagcgtggcg ggcccccgct gaaaggtaga ccggcgagtg gctgtcgacg
    12901 ggcgggggct tgctgaatga cggccgttcg ctgaatattc ccgcaagttg cagtaaccta
    12961 gagggtccgc gttttttgcg gacttcattt ttcggaaaat cctcaggatc atgacttcct
    13021 cgcccccgtc gcccctgttt tcgcagcgcg cgcgcaaact caccggttcc gccatccgtg
    13081 aaatcctgaa aatcacgcag cgccccgaca tcatttcgtt tgccggcggt ttgccggcgc
    13141 cgcaggggtt cccggttgag gaactccata cggccttcga tcgcgtcctg agctcctcgg
    13201 cgcaggtcgg cctgcagtac ggccccacgg aagggtatca acccttgcgg gaatgggtgg
    13261 cgcaagacat ggtggcgcgt ggcgccgagg tgtctcccga ggaagtgctc atcgtgtccg
    13321 gctcgcagca ggcgctagac ttgctgggca agctgttcat cgatccgggc agcaagatcc
    13381 tgatcgaagc ccccagttac ctgggcgcca tccagtcatt ctcgctgttc gaacccgaat
    13441 accgggcgat acccaccgat ggtggcggtc tgattcccga cgggctcacc gatgactgcg
    13501 tgcggggcgc gcggttcgtc tatgcgctgc ccaatttcca gaaccccacc ggcctgacac
    13561 tgagcctgga gcggcgccgc gcgctggtgg cgcgctgcgc cgcggcgggt ctgcccattg
    13621 tcgaggacga cccgtacggt gatttgcgct acgcgggcgt cgcacaaccc tccctgctga
    13681 acctggggcg cgaagccggc gccaccgtga tccgcatggg cacgttttcc aaggtgctgt
    13741 cgccgggcct gcgcctcggc tacatcgtgg cgccgcgccc cctcatcgcc aagctggccc
    13801 agatcaagca ggccaccgat ctgcatacgg cgatgctgac gcagatggcg gtgtacgaaa
    13861 ccgtgaagaa tggcttcctg gcccggcacc tgccgggtat ccgcgagctg tacaagaatc
    13921 agtgcggcca catgctcgat gccatggcgc gcgatttccc ggattccgca acctggacac
    13981 gcccggaagg cggcatgttc atctgggtca ccctggcgcc gcaccttgac gccggccagt
    14041 tgctccagac cgccgtggaa cgcaaagtgg cattcgtgcc gggtgcgcca ttcttcgctg
    14101 gcgtggtgcc gcaactcaat acgctgcgcc tgagtttcgt cactgtgccc gagccgaaga
    14161 ttcgcgaagg catcgccacg ctgggtcggt tgctcaagga agcctgagat cgtttcgcgc
    14221 gttccgtcgc gattgtttta tgacttccgc ttcaaattcc ccttcgtcga gtcaccaact
    14281 cgatgcatcc attcccgacc tgtcggacat gcggccggac gactgggtcg agcattggtt
    14341 gcctcgctcg tgggggccgt acgcccggct atgccggctc gatcggccgg tcggtacctg
    14401 gctgacgctg ttgccatgcc tggcggcggt ggtgcaggcg gccgacgggt ggccggcatt
    14461 gttcaggctg ttcatttttt ccctgggcgc cctgctgatg cgcggagtgg gatgcaccat
    14521 caacgacatc tgggaccgcg atttcgacct gaaggttgag cgcacgcgct tcaggccgct
    14581 gaccagtggc caggtcacgc tgcgcgcggc ggtggtgttc ctgttcgccc agcttgccgt
    14641 gtgcgccctg ctgctgctga ccatcaatgc gtacagccgg tggctggcgg tcgcactggt
    14701 gcccatcgtc atcatctatc ctttgtgcaa gcgcttcacc tactggccgc aggtcgtgct
    14761 gggcataggt ttcaactggg gcatgctgat ggcctggtcc gacacacaga acgtggtccc
    14821 gctggcggcg gtggcaatgt gggtcggcgc ggtgctgtgg caggtgggct acgactccat
    14881 ctatgcctac gtggatgtgc gcgatgatcg catgctgggc ctgcattcca cggccatgcg
    14941 cttcgccgac cgtggcaagc ggtggatcac cggcttctac gtggcgacgg tcgtgttgtg
    15001 gttgtacgcc ggcatcgcgc tgaacatggg ttggggctat ttcatcgtgc tcgcggtcat
    15061 caccggtcat ttcatctggc agatgcgcct tttcagcctc gagcgtcccg atcggaattt
    15121 catgctgttc cgcgccaata tctgggtggg cgtgctgctg ctggtggccg ccctggcggg
    15181 cacggtgatt tagagatcct ccgaacaagt cgtcgcgcca tgcctgattt ctgcagtagc
    15241 atatgtggca gacatcaggc cctgacgttc ccgttccttt tctaaagagc acatggcccg
    15301 acgtgtcgga gggtatcggc gcgtcggcac cccggggggc gcgatatgcc gccgactggc
    15361 cccggcgctc aacaaacaga aaggagacag gctcatggcg gatcaaggcc cggaacaggc
    15421 ggatctggaa gtcgactaca tcatcgtggg ggctgggtct gccggctgtc tgctggcgaa
    15481 caggctctca gcggacccat cgcgcaaggt gttgctgctt gaagcgggcg ggcgcgatga
    15541 ctggcgctgg atccatattc cggtgggcta tctgtactgc atcggcaatc cgcgcactga
    15601 ctggtgttat cgcacgcgcc ccgatccggg catcaacaac cgtgtgctcg gttacccgcg
    15661 tgggcgagtg ctgggcggca gttcgtccat caacggaatg atctacatgc gtggccaggc
    15721 cgccgattac gatggctggg cggcactggg caacacgggc tggtcatgga gcgacgtgct
    15781 gccggtgttc aagcaggtcg aagaccacta tgccggcgaa agcgaattcc atggcgcctc
    15841 gggcacctgg cgggtggaac agcagcgcct gtcctggccg attctcgatg cgttccgcga
    15901 ggctgcggcg caggcgggaa tccgcaaagt cacggacttc aatcgcggcg acaacgaggg
    15961 gtgcggctat tttgaagtga accagcgtca gggggtgcgc tggacctcgg ccaaggcgtt
    16021 cctgcatccc gttgccaatc gcgccaacct cacggtgttg acggatgcgc aggcgcaggg
    16081 cctggtgctg tcggggcggc gcgccgaggg ggtgcgcttc actcatggcg ggcagtccaa
    16141 gttcgcgcgc gcccgcgccg aggtgattct gtccacggga tccatcgggt cggcgcagtt
    16201 gctgcagctc tcgggcatcg gccacggccc gctgctccaa tccctgggta ttccggtcgt
    16261 gcatgacctg aaaggggtgg gggagaacct gcaggatcac ctgcagttgc gcctgatcta
    16321 tcgcgtgcag ggcgcgaaaa ccctgaacac catggccaat tcgctatggg gcaaggccgt
    16381 gatgggcttg cagtatgtgt tccagaaacg ggggccattg acgatggcgc cttcgcagat
    16441 gggggcgttc gcgcgttccg atgcgcaacg atcgcgcgcc aacctggaat atcacgtgca
    16501 gcccttgtcg ttggaaaaat tcggcgacac gctgcacgac ttccccgcct tcaccgcgtc
    16561 ggtctgcaat ctgcagccgt ccagccgcgg ccacgtccac atcacgtcgt ccaacccgca
    16621 agaggcgccg gccatcctgt gcaactatct gcagacg
//
LOCUS       DASTNK010000104         3675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_814267, whole genome shotgun sequence.
ACCESSION   DASTNK010000104 DASTNK010000000
VERSION     DASTNK010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814267"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VFR20_07810"
     CDS             <1..525
                     /locus_tag="VFR20_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEU0230462.1"
                     /translation="DFGLGQLLGTRQIRKMIASYVGENKEFARQFLAGELELEFTPQG
                     TLAEKLRAGGAGIPAFFTRTGVGTLVAEGKEVREFDGHKYLMERSLVPDVSLVKAHTA
                     DRSGNLVFRKTARNFNPNVAMAGKITIVEVEKLVDIGDIDPDGVHLPGIYVHRIVVNA
                     HPEKRIEQRTLRNA"
     gene            543..1196
                     /locus_tag="VFR20_07815"
     CDS             543..1196
                     /locus_tag="VFR20_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEU0230463.1"
                     /translation="MAWTRDEMAARAARELKDGFYVNLGIGLPTMVANHVPPGVEVWL
                     QSENGLLGIGPFPTEEELDADLINAGKQTVTTLPGSSIFASADSFAMIRGGKIDIAIL
                     GAMQVSEKGDLANWMIPGKMIKGMGGAMDLVGGVGKVVVLMEHVARKKDGTEDIKLLP
                     ECTLPLTGVGVISLVITDLGVMEVTPSGLKLTELAPGVSVADIQAKTTAKLDVSAVA"
     gene            1283..3085
                     /locus_tag="VFR20_07820"
     CDS             1283..3085
                     /locus_tag="VFR20_07820"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU0230464.1"
                     /translation="MLVVGNSIFAERIGALRGVLAAQGADACVVLSSDPHLSEYLPPR
                     WQTRQWLSGFEGSAGTLVVTADFAGLWTDSRYWEQADKDLDGTGIQTMRAGADNVPGV
                     VDWLLTAVPEGGAVSVDGQVMDVHTHRDWSARLAGRSIRLVCEADLPDRIWADRPGLP
                     FAPVYEHQPPFACRKRSDNLAQVREAMRAHGATWHWISSLDDIAWLLNLRGADVSYNP
                     VFLAHALIGSDSATLFVTPEKIAPALRATLERDGVRVEPYDSASSALADLPQGDTLLI
                     DPARTTAGILEAASTVTMIEATNPSHMFKSRKNAAEIDNVRRTMEQDGAALCEFFAWF
                     EAAQGKERITELTVDEKITAARARRPNFVCPSFSTIAAYNANGAMPHYHATESSHAEI
                     SGNGLLLIDSGGQYLGGTTDITRVVQVGQASVEQKRDYTAVLKGMIALSQAVFPRGTA
                     SPMLDTLARSPIWKTGADYGHGTGHGVGYFLNVHEGPQAIAYRASVTPQRGMEPGMIT
                     SNEPGLYRPGRWGIRIENLVLNRPACETEFGEFLEFETLTLCPIDTRCIDTGLLSASD
                     RDWLNGYHDEVRRRLMPLVEGAAREWLLERTQPQ"
     gene            complement(3079..>3675)
                     /locus_tag="VFR20_07825"
     CDS             complement(3079..>3675)
                     /locus_tag="VFR20_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp/Glu racemase"
                     /protein_id="HEU0230465.1"
                     /translation="ELKAMDADSDRCALELSDARVDVLGYACLVAIMSMGLGYHRESE
                     KRLHQVTVDNHNPAPVVSSAGALVDGLHAMGAKKVSILTPYMKPLTQLVVDYIENEGI
                     EVHDAVSLEIPDNLEVGRQNPLAPAEHIKRVNTKGVDAVVLSACVQMPSLQSVQQVED
                     HLGIPVTTAAVSTVYQMLKRLDLRREVPKAGSLLSGRY"
BASE COUNT          669 a         1148 c         1185 g          673 t
ORIGIN      
        1 gatttcgggt tgggccaact gctgggcacg cgccagatcc gcaagatgat cgcgtcctac
       61 gtgggtgaga acaaggaatt cgcgcgccag ttcctggcgg gtgaactcga actggaattc
      121 acgccgcagg gcacgctggc ggaaaaactg cgtgccggcg gggccggcat tccggcgttc
      181 ttcacgcgca ccggagtggg cacgctggtg gccgagggca aggaggtgcg cgaattcgac
      241 ggccacaagt acctgatgga acgatcgctg gtgcccgacg tgtccctggt caaggcgcat
      301 accgccgacc gcagcggcaa cctggtgttc cgcaagacgg cgcgcaattt caaccccaac
      361 gtcgccatgg ccggcaagat caccatcgtc gaggtcgaga aactggtgga tatcggtgac
      421 atcgatccgg atggggtgca tctgccgggc atctacgtgc accggatcgt ggtcaatgcg
      481 catcccgaaa aacgcatcga acaacgcacc ctgcgcaacg cctaaggaca aggagatcga
      541 aaatggcttg gactcgtgac gaaatggcgg cgcgcgccgc ccgtgaactg aaagatggct
      601 tctacgtgaa cctggggatc ggcctgccca ccatggtggc gaaccacgtg ccaccgggag
      661 tggaggtctg gctgcagtcc gaaaacggct tgttgggcat cgggcctttc cccactgaag
      721 aggaactcga cgccgacctg atcaacgccg gcaagcagac ggtcaccacg ctgccaggtt
      781 cgtccatctt cgcgtcggcg gattcgttcg ccatgattcg cggcggcaag atcgacatcg
      841 ccatcctggg cgccatgcag gtgtcggaaa aaggtgatct ggccaactgg atgattcccg
      901 gcaaaatgat caagggcatg ggcggcgcca tggatctggt gggtggcgtg ggcaaagtcg
      961 tggttctgat ggagcacgtc gcgcgcaaga aggatggcac cgaagacatc aagctactgc
     1021 cggaatgcac gttgccgctg actggcgtgg gcgtgatcag cctggtcatc accgatctgg
     1081 gcgtgatgga agtcaccccc agcggactca agctcaccga gctcgcccca ggcgtgtcgg
     1141 tggccgacat ccaggcgaaa accacggcga agctggacgt ctcggcggtg gcctgattcc
     1201 gcttttgtcg ctcgaaccct ggccggtctt cccttgaccg gccagccgtt cgcgccgttg
     1261 ctcattcgtt tctagtcaaa ggatgctggt tgtgggaaat tcgatctttg ccgaacgcat
     1321 cggcgcgctg cgtggtgtcc tggctgcaca gggggccgac gcctgtgtcg tgctgtcttc
     1381 cgatccgcat ctttccgagt acctgccccc ccgctggcag acgcggcagt ggctatcggg
     1441 gttcgagggc tccgccggca cgctggtggt cacggctgat ttcgcggggt tgtggaccga
     1501 cagccgatac tgggagcagg cggacaagga tctggatggc accggcattc agaccatgcg
     1561 ggccggggcg gacaacgtac ccggcgtggt cgattggctt ctgacggcgg tgcctgaagg
     1621 gggcgccgtc agcgtcgacg gccaggtcat ggatgtgcac acacatcgtg actggtcggc
     1681 ccggttggct ggccgttcca tccgcctggt ctgtgaggcg gacctgcccg accgaatctg
     1741 ggccgatcgg cccggcctgc ctttcgcccc tgtctatgag catcagccgc cgttcgcctg
     1801 ccgcaagcgt tcagacaacc tggcccaggt gcgcgaggcg atgcgcgcgc acggcgcgac
     1861 ctggcactgg atttcctcac tggacgacat cgcctggctg ctcaatctgc gtggcgccga
     1921 tgtgtcctac aaccccgtgt ttctggcaca cgccctgatc ggttccgatt ccgccacgtt
     1981 gttcgtgacg cccgaaaaaa tcgccccggc gttgcgcgcc acgctggaac gcgacggggt
     2041 gcgggtcgaa ccctacgaca gcgcctcatc cgcgctggcc gacctgcccc agggcgatac
     2101 cctgctcatc gatccggccc gcaccacggc cggcatcctg gaggcggctt ccaccgtgac
     2161 gatgatcgaa gcgaccaacc cgtcgcatat gttcaagtcg cgcaagaacg ccgccgagat
     2221 cgacaacgtg cggcgcacca tggagcagga tggcgccgcg ctgtgcgagt tcttcgcctg
     2281 gttcgaagcg gcgcaaggca aggagcgcat caccgagttg accgtggacg aaaagatcac
     2341 ggcggcgcgg gcgcgccggc ccaatttcgt ctgccccagt ttttccacca tcgccgccta
     2401 caacgccaat ggcgccatgc cgcattacca cgccacggaa agctcacacg ccgagatttc
     2461 cggcaatggc ctgctgctga tcgattcggg cggccaatac ctgggcggca ccaccgatat
     2521 cacccgcgtg gtgcaggtgg ggcaggccag cgtcgagcag aagcgcgact acacagcggt
     2581 gctcaagggc atgatcgcgt tgtcccaggc ggtgttcccg cgcggcaccg cgtcgcccat
     2641 gctcgacacg ctcgcccgct cgcccatctg gaagaccggg gccgattatg gccacggcac
     2701 ggggcatggg gtagggtact tcctgaacgt gcacgagggg ccgcaagcca tcgcgtaccg
     2761 tgcgtccgtg acgccgcagc gcggcatgga gccggggatg atcacctcca acgagccggg
     2821 tttgtaccgc cccggccgct ggggcatacg tatcgagaat ctggtgctca accgacccgc
     2881 gtgcgagact gagttcggcg agttcctgga gttcgaaacc ctgacgctgt gtcccatcga
     2941 tacccgctgc atcgataccg ggttgctgtc tgccagcgac cgggattggc tcaatggcta
     3001 tcacgacgaa gtgcggcggc gtttaatgcc gctggtcgag ggtgccgcgc gggaatggtt
     3061 gctggagcgt acgcagcctc aataacggcc tgacagcaag ctgcccgctt tggggacttc
     3121 ccggcgcaga tccagacgct tgagcatctg gtagacggtg ctgacagcgg ctgtcgtgac
     3181 aggaattccc aggtggtctt ccacctgttg caccgattgc agggaaggca tttgtacaca
     3241 ggcggacagc accaccgcat ccactccctt ggtgttgacg cgcttgatgt gttccgcggg
     3301 tgccagagga ttctggcgac ccacttcaag gttatcggga atttccagcg acaccgcatc
     3361 gtgtacctcg atgccttcgt tttcgatgta gtccaccacg agttgcgtga gtggcttcat
     3421 gtagggcgtc aggattgaaa ccttctttgc ccccatggca tgcaggccat cgaccaacgc
     3481 gcccgcgctg gatacgacag gcgccgggtt gtggttgtcc accgtcacct ggtgcaggcg
     3541 tttttccgat tcacggtggt agcccagccc catgctcatg atggcaacca ggcaggcgta
     3601 gcccaggaca tcgacgcgcg catcggacag ttccagtgcg caacgatcag agtcggcatc
     3661 catggccttc aattc
//
LOCUS       DASTNK010000105        15351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_817383, whole genome shotgun sequence.
ACCESSION   DASTNK010000105 DASTNK010000000
VERSION     DASTNK010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15351
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817383"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VFR20_07830"
     CDS             <1..586
                     /locus_tag="VFR20_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU0230466.1"
                     /translation="RPYKVVKYATDITRRFTAAQALSDAVEDLKQNAGRASEANGLAN
                     EASGVARQGGENVQSVVSTMQTITDSSRRISEIIGVMDNIAFQTNILALNAAVEAARA
                     GQHGKGFAVVAAEVRSLAQNSATAAREIRSLIMTSVEQIDNGAVQVQSAGKIMKDIMA
                     SSHRVTEIMKKVVDASLAQSMKLDQVTADLTRAA"
     gene            complement(896..1351)
                     /locus_tag="VFR20_07835"
     CDS             complement(896..1351)
                     /locus_tag="VFR20_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011295775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HEU0230467.1"
                     /translation="MQLYRIASSRHPIWDGTGAALLGGRWNSPGRPVIYASMTYAGAM
                     LEVLAHTNIGKVPPSHQCVAANVSDELSTTRYLLEGLPPGWDLEDSAGARKIGDAWLD
                     ACKTALLIVPSVVARLEWNVLVNPRHSDAIRIRVSDPEVVRWDQRLFSK"
     gene            complement(1352..1780)
                     /locus_tag="VFR20_07840"
     CDS             complement(1352..1780)
                     /locus_tag="VFR20_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008344738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HEU0230468.1"
                     /translation="MLALRADQIGTIMGLEPLPKTFAELDATVANGIRKAALRAIVGQ
                     LVERREQILPLLYRIVPEGTFKRRKGRLSPEESERTERLARVFATAAYVLESNEDARR
                     FLHAPHPMLEGRAPLDVAFTEIGAQRVERILWAVHHGLAA"
     gene            1858..2088
                     /locus_tag="VFR20_07845"
     CDS             1858..2088
                     /locus_tag="VFR20_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0230469.1"
                     /translation="MNYDLVTLDLLVTVAECKNLTRAAQRKHLAPSAVSKRIQEFEER
                     VGAVLLARNSRGVELTPEGQSIVFHARKPQGV"
     gene            complement(2350..3639)
                     /locus_tag="VFR20_07850"
     CDS             complement(2350..3639)
                     /locus_tag="VFR20_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0230470.1"
                     /translation="MDKDIEKRTMRRISWRIVPFIMILYFVAYIDRVNLGFASLTMNA
                     DLGFSASILGFGAGIFFWGYFLFEVPSNIVLHKVGARLWIARVMLTWGVISGAMAFIQ
                     GPTSFYVVRFLLGAAEAGFFPGIILYLSYWFPARHRAGVTALFMAAAPLSTALGSPIS
                     AAFLQMHGFMGLAGWQWLFIAEAVPALVLAVVVFFYMTDKPEQAKWLKDDERAWLINA
                     MREEASTVKNAKHGLLSGVTNPRVLALALIYFGTSAGLYTLGIWAPQIIRHLGVSSMT
                     VGFLNAIPPIVSVIAMVLWSRHSDRTGERTWHVVLACLAAAIGLIVAAGANSIVGLIS
                     ALTLVNIGISCAKPSLWTMPTTFLSGAAAATGIATINSIGNLGGFAGPAMIGWVKDHT
                     GSFSGGLYFVAGLLVLSAVLTLLLARSGRMAVAAAES"
     gene            complement(3735..5210)
                     /gene="pyk"
                     /locus_tag="VFR20_07855"
     CDS             complement(3735..5210)
                     /gene="pyk"
                     /locus_tag="VFR20_07855"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU0230471.1"
                     /translation="MARRRNAKIIATLGPSSSDRKTIEALYHAGADVFRLNLSHGTHA
                     DHQQRYDTIRAIERDVGSPIGILLDLQGPKLRIGVFTNGPITLEPGAAFRLDLDTASP
                     GDATRVALPHPEIFTALRPGTALLLDDGRIQLKVERCGPDLAQTTVITGGVLSERKGV
                     NIPDVILPVAAITEKDRRDLEFGLSLGVNWVALSFVQRVEDILEVRRIVKDRAGILAK
                     LEKPAAIRDLEGIVEAADAVMVARGDLGVEIPPERVPAIQKQIVRACRNAGKPVIIAT
                     QMLESMVGTPVPTRAEASDVATAVYDGVDAVMLSAESAAGGFPIESVRMMDRIICRTE
                     DDPHYREGIVASRCAPRTATSADAIGHAIRHITTLLNPVANVAYTSSGYSAMRLARER
                     PLTRIISMTPHEAVAQRLALVWGVHPVVSPLVVDPLEVSELACDTVRKEGLGAPGQTV
                     VVSAGMPFGIPGTTNLLHIATIRRVEASQNDTQARPQVFQR"
     gene            complement(5214..6527)
                     /locus_tag="VFR20_07860"
     CDS             complement(5214..6527)
                     /locus_tag="VFR20_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU0230472.1"
                     /translation="MSEPYEAVLPCPTVENSVEHLLDDMFQAAIAAAQPARRIPAFLP
                     APPRGRTLVIGAGKASAAMAKAVEDHWPAPLAGLVITRYGYGVPCARIEIVEAAHPIP
                     DANGHRATKRMLEMVSGLSADDLVICLISGGGSSLLPLPAEGIGMDTKQDINQALLAS
                     GATITEMNCVRRHLSAIKGGHLAAACHPARVVNLIISDVPADTVSDIASGPTVPDPTR
                     CSDALDILRRYKIPVPATVIAMLEKGLTETLKPGDARLPPIDTHVIATPQMALEAAAA
                     VARERGVDAYILGDSIEGEARDVGVVMAGIARQVRRHGQPFRAPCVLLSGGETTVTVT
                     GAGCGGRNVEFLLSLGLALSGEPDIFSIAGDTDGADGKADIAGARLRPDSLAHARSLG
                     ISPIHRLEDNDGHGFFQALGDSIITGPTLTNVNDFRAIYIGRTGE"
     gene            complement(6628..7710)
                     /locus_tag="VFR20_07865"
     CDS             complement(6628..7710)
                     /locus_tag="VFR20_07865"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006411801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEU0230473.1"
                     /translation="MKTYRIATIPGDGIGKEVVPAGQQLLASLARASGQFAFEFENFG
                     WGADYYRQHGVMMPADGLDDIRDKDAILFGSAGDPDIPDHITLWGLRLKICQGFDQYA
                     NVRPTRILPGIDAPLKRCRPEDLDWVIVRENSEGEYSGIGGRVHQGHPLEAATDMSIL
                     TRAGVERIMRFAFRLAQSRPRKLLTVITKSNAQRHAMVMWDEVAKLIALEFPDVTWDK
                     ELVDAATARMVNRPASLDTIVATNLHADILSDLAAALAGSLGIAPTGNIDPERRYPSM
                     FEPIHGSAFDIMGKGLANPIGTFWSVVMLLEHLGETQAAARVMDAIEAVTARPELHTR
                     DLGGRATTAEVTAAVCALVENASATA"
     gene            7893..8810
                     /locus_tag="VFR20_07870"
     CDS             7893..8810
                     /locus_tag="VFR20_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006397928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0230474.1"
                     /translation="MTDTVQPADFGFFSTLVSLGSLSAAARELGLTPAAVSKRLTQME
                     QRTGVPLVNRTTRRMMLTPEGEIYLEYARRVLDEVDALSELLGGAKTRPMGLLRVNAT
                     LGFGRNHVAPVISRFVSAYPQVSVQLQLSVTPPPLTADVYDVCVCFGEPPDARVIARR
                     LAPNRRLLCAAPGYLARHGIPLTPRDLTKHHCISIRQGDEAYGVWRLRNGREALAKSE
                     AIRIHGPLTTNDGEIAVKWALDGHGILMRAEWDIKDYLADGRLVTVLPDYATPAADIY
                     AVYAQRHQFSTRIRAFVGFLADALFPRQR"
     gene            complement(8748..9017)
                     /locus_tag="VFR20_07875"
     CDS             complement(8748..9017)
                     /locus_tag="VFR20_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230475.1"
                     /translation="MSRVLVDSNVLLDVLTDDPQWGAWSAAQLDASAAQGELCINPII
                     YAEVSVGFEHIEDLDATLTPDVFVRLPLPWEERVGQKAHKGAYPR"
     gene            complement(9263..9341)
                     /locus_tag="VFR20_07880"
     tRNA            complement(9263..9341)
                     /locus_tag="VFR20_07880"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9303..9305),aa:Ile,seq:cat)
     gene            9438..9965
                     /locus_tag="VFR20_07885"
     CDS             9438..9965
                     /locus_tag="VFR20_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0230476.1"
                     /translation="MPAGAKCDFNGICRMIRFWFYPIEAIGGFAMTDDRVITLGKLSI
                     RYLIDGSQTKTMGMFELTVQPGANVPPPHSHANNEEIVYVLAGKLRYRVGDDTRDLQP
                     GGSMHTPKGVVHAFANPFSDVAHALIVLSPDIGAQYFRDIGEAIGAGGPPDKAALMAV
                     MKRYGLVPALPPAAS"
     gene            10224..>15351
                     /locus_tag="VFR20_07890"
     CDS             10224..>15351
                     /locus_tag="VFR20_07890"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEU0230477.1"
                     /translation="MARFATLVLLWLVGVAPSWAARVAQVSPQGRVSVAESVKITFDS
                     PVIHFGDGQAHAPATVKCDDPALKGSGRWLDAERWTYVFQSRPGPGVTCHVEIDPAFR
                     ALDGKPVEGATHFQFDTGAPTIRTVRPYGSSQIDEEQMFVLEFTGAVNKDSLLAHASC
                     LAEGIGEAIPVRLITGTERENVLKAAVYDKENRGPGSLVLQCKRRLPANASVQLRVGP
                     GVASPSGIASTQPAIFKYKVRPPFTVSFSCQRENAHAPCTPVLPISLSFSAPIPADQA
                     AKIRLKTGKGERSPDKGDYGYRNSVEVVRFKGPFPPDADVTLVLPPDLKDDSGRPLSN
                     ADQFPLTTHMASFPPLVKFAKPTFGVIERFANVPSGGSEADYPPSVPLTVRNVEADLR
                     TRDLLVSSGEIKDYVVRDDADVLHWYERLIKLNEAYLTQRQIDEVMHDRPVPENKKRG
                     EAVIDTRGVSVLNGQSGGRQLKLPAPPKSGTRPFEVIGVPVPRLGFHVLEVESPRLGQ
                     SLLGEDKPMYVRSSALVTNLGVHIKQGRDDLLAWVTTLDQGKVVPGAAITVLSCKGTV
                     LYKGKTDARGIWHKRGVDDIPQCDSGFNGIYVSARITKDNPVAQGKADFAFALSTWND
                     GIEPWRFNVPTDNSPYRTTLVDTVYDRPLFHVGETVSMKHFLRVETRDGLAVPTDSDP
                     GPDRIVISLEGSDQRYTQHLDWKATPTGGLSAVSQFVIPKSAKVGTYSVTYQDANGTW
                     FGSSSFRVAAFKLPVYQGTLEVSDKAGSHTLIAPTSVHADVQMSFVSGGPAGKLPVTL
                     SGVVQENWLNFDGYDDYSFNVPLSTNPDLDSDYSGADPADTATHDRQALFLDKKPVTL
                     DAHGGARIVLDSLPKVTRPQNWLFEASYPDPNGEIQTLSHTVAVWPAAVVVGLRTGSW
                     VQKGKATPISVVALSPAGVPQAGVPVSVDARQKTTYSTRRRMVGGFYSYDNHVVVKSM
                     GTVCQGKTDSHGKLECSITLTHSGSIQLLAHAKDAQGRQASAQNTLWVIGGDDLWFGG
                     SNDDRMDVIPEKKIYKPGETASFQVRMPFHEATALVAIEREGVLSARVVHLEGDNPTV
                     KIPVQAKWGPNVYVSVLALRGRLREVPWYSFFSWGWRHPADWYDAYNNENKEFKAPTA
                     FVDLSKPSFRFGLTQIKVSDQRDALNVKVTADKPVYQVRGKANVTIHVTKPDGTPAAN
                     GQVAFAAVDEALLQLAPNNSWDLLGAMRQSRSYGVRTATAEMQVVGRRHYGRKAVPAG
                     GGGGKSPTRELLNTLLLWKPMVQLDANGEAHLAVPLNDSITRFRLVAIADYGQERFGT
                     GSTDIRVTQDLQAISGLPPLVREGDAYQAMVTVRNATTRDMKVDVGLGYSGKGVPGET
                     LPSKVVDVAAGTAQGVTWRVKMPEADLPDDQTRLDWRIQAREQGTGHAADDLVVHQQL
                     IPSVPVAVTQAALIGVDAASRPLELPVQAPDKALVGTNGRPRGGLTVTMRSSLAGGLP
                     GVRHWLAVYPFSCLEQRTSKAIGMRDPAQWRDLISRLPNYLDNDGLASYFPGATHGSE
                     VLTAYVLEASDEASALGMPFTLPDADKRKMLDGLRAYAQGRIVRHHWAPQKDLDVRKL
                     LVIEALSHDREATVRMLDSIQITPNRWPTSAVINWLSLLQRMPDIPNRTAQLAKARQI
                     ILGRMLSRGQSLVFPDDDLNNWWWLMDNRAVNSARLLLTVA"
BASE COUNT         2884 a         4585 c         4978 g         2904 t
ORIGIN      
        1 ccggccctac aaggtggtga aatacgccac agacattacc cgacgcttta ccgcggcgca
       61 ggctctgagt gacgcggtcg aggatctgaa acaaaatgcc ggtcgcgcct ccgaggccaa
      121 cggcctggcc aacgaagcct ccggcgtcgc gcgccagggt ggcgagaacg tgcaaagcgt
      181 cgtctccacc atgcagacca tcaccgatag ttcgcgccgg atttccgaga tcatcggcgt
      241 gatggacaac attgcctttc agaccaatat tcttgctctg aacgctgccg tcgaggccgc
      301 gcgagcgggc cagcacggca agggcttcgc cgtggtggcg gccgaagtcc gcagcctggc
      361 tcagaacagc gccacggcgg cacgcgagat ccggagccta atcatgacgt cggtcgagca
      421 gatagacaat ggggcggtgc aggtccagtc ggccgggaaa atcatgaaag acatcatggc
      481 ttcctcacac cgggtgaccg agatcatgaa gaaagttgtt gacgcgtctc tggcccagtc
      541 catgaaactg gatcaggtga cggctgatct gacgcgggcg gcgtgagggg ggtcgggttc
      601 ggcgaccggg gcacggacgc agccgcgtgg cgatgcgaag aacgtgctgc agcgccttac
      661 cgacaggcgc ttcgctattt tgacgtgtgt tgatagccga cgccggtgtc gagggttccg
      721 tagaactgca cgcccgattg tgacgcggaa cccgcgccac gcgacgaggg gccggtgcaa
      781 gccccgagcg taatgagaat tgcgaggcaa aagagcagtt ttgcaatcat ggggtttatc
      841 catcccgccc gtgcgggcca tacgtatcgt acgtgtttaa cgcgccattt acaggttatt
      901 tcgaaaacaa cctctggtcc caacgcacga cctccgggtc ggagacccgt atcctgatgg
      961 cgtccgaatg ccgtggattg accagcacgt tccattcgag gcgtgcgacc accgatggta
     1021 cgatcagcag cgctgttttg catgcgtcaa gccacgcgtc gccgattttc cgggcgccgg
     1081 cgctgtcctc gagatcccag ccagggggca ggccctcgag caggtatcgc gtagtggata
     1141 gctcgtcgga cacgttggcc gccacgcact ggtgcgacgg aggcactttg ccgatgtttg
     1201 tgtgcgcgag cacctcaagc attgcaccgg cataggtcat tgaagcatag atcaccggtc
     1261 tgcctgggct gttccagcgc ccacccagca gcgccgcccc ggtgccgtcc cagattgggt
     1321 ggcgcgagct tgcgatacgg tacagctgca tctaggcggc aagcccgtga tggacggccc
     1381 aaaggatgcg ctccacgcgc tgggcgccga tctcggtgaa ggccacatcc agcggtgcgc
     1441 gcccttcgag catcgggtgc ggcgcatgga gaaaccgcct ggcgtcttcg tttgactcga
     1501 gcacataggc ggcggtggcg aagacccgcg ccaggcgctc ggtgcgttct gattcctcgg
     1561 gactcaggcg tcctttgcgg cgcttgaatg tgccctctgg cacgattcgg tacagcagcg
     1621 gcaggatctg ctcgcggcgt tccaccaact gcccgacgat ggcccggagg gcggccttgc
     1681 ggataccgtt ggccaccgtc gcgtcaagtt cagcgaacgt tttcggcagg ggctcgagcc
     1741 ccatgatggt gccgatttga tcagcccgaa gcgcaagcat ggtaccctct gaactagtat
     1801 tgaatgatgc attaatgcta gatcagttga tactttttga caagtgagtg cccagccatg
     1861 aactatgact tggtcacgct ggatcttctg gtgacggtgg ccgagtgcaa gaacctcacc
     1921 cgggccgccc agcgcaaaca tttggcgcct tcggcggtca gcaagcgtat ccaagagttt
     1981 gaggagcggg ttggcgcggt actgctggcg cggaattccc gtggcgtgga gctcacccca
     2041 gaggggcaat ccatcgtgtt tcatgcccgc aagccgcagg gtgtttgact tcaaaatccg
     2101 gatcagcagt tttgaatgca tgtgccagat ggtcggggcc agcctctacg ccgcattgag
     2161 ccgggcttcc gcctcatagg cttgtgggtt tcctggggcc gtgattcacg ggctttgtgt
     2221 gacgtccgct tcccatgcct gaacagcctg gcaccttgtg caccggtgcc aggctttgta
     2281 tgactacagc atggttctat cagtcaagaa tcctaaggaa aaatgggatc cgcacgcctt
     2341 gggcaaatct cacgattcgg ctgccgcgac agccatcctc cctgaacgcg ccagaagcag
     2401 cgtcaggaca gcggagagca caagcaaccc ggcgacgaaa tacaggccac cgctgaaact
     2461 gcctgtatga tccttgaccc atccgatcat ggccgggccg gcgaatcccc cgagattgcc
     2521 aatggaattg atggtggcga tgccagtggc ggccgccgcg cctgaaagga acgtggtggg
     2581 catggtccac agtgacggct tggcgcagct gattccgata ttgacgagcg tcagagcgga
     2641 aatcaagccg acaatgctgt tggcgccagc cgccacaatc agaccgatcg ccgctgccag
     2701 acaggccagc accacatgcc aggtgcgctc gccggttcgg tcggaatggc gggaccacaa
     2761 gaccatcgcg atcacggaaa caatgggggg aatggcattc agaaagccaa ccgtcatgga
     2821 cgagacgccc agatgcctga tgatctgggg agcccatatg ccgagtgtat acaggcccgc
     2881 cgaagtaccg aagtagatga gggcgagagc cagcacacgc gggttggtca cgccgctcag
     2941 gagcccgtgt tttgcgttct ttaccgtcga cgcttcttca cgcatggcat tgatcagcca
     3001 ggcgcgctca tcgtccttca gccatttggc ctgttcaggc ttgtcggtca tataaaagaa
     3061 aaccaccacc gcgagcacga gcgccgggac cgcctccgcg atgaaaagcc attgccagcc
     3121 cgccagaccc atgaatccgt gcatctgcag gaacgccgcg gagatcggtg agccgagcgc
     3181 ggtggacagt ggcgccgccg ccatgaacaa cgcggtgacg cccgcgcggt gacgcgcggg
     3241 gaaccagtag ctgaggtaga gaatgatccc ggggaagaaa ccggcttcgg cggcgccgag
     3301 caggaatcgc accacataga agctggtcgg cccctgaata aaagccattg ctcccgaaat
     3361 gacgccccac gtgagcatga cgcgggcgat ccacaatctg gccccgactt tatgcaatac
     3421 gatgttcgaa ggcacttcga acaggaagta gccccaaaaa aaaataccgg cgccgaaacc
     3481 gagaatggag gcggagaagc ccaggtccgc gttcatggtc agcgatgcga aacccaggtt
     3541 cacgcgatcg atgtaggcaa cgaagtacag aatcatgatg aagggtacga tgcgccagct
     3601 gatccgccgc atcgtgcgtt tttcaatgtc tttatccaca gaatgtctcc tttcttctat
     3661 gttggtcagt cgtgtaccga tcacgaaata tgaaatgccg cgtcgcggga gatatccgac
     3721 cccgctcccg gcggttaccg ctggaaaacc tgaggtcttg cctgggtatc gttttggctg
     3781 gcctccactc gccgaattgt cgcgatgtgt agaagattgg tcgtgcctgg aatgccaaac
     3841 ggcatgccgg cggaaacgac cacggtttgc cctggcgcac ccaacccctc cttgcggacc
     3901 gtgtcgcatg ccagttcgct gacctccagg ggatcgacca ccagggggct gaccaccgga
     3961 tgcacccccc agacgagggc caggcgttgc gcgacggctt cgtgaggggt catgctgatg
     4021 atgcgggtga ggggccgttc gcgcgcgaga cgcatggccg agtagccgga gcttgtatag
     4081 gcgacgttgg cgactggatt cagcagcgtg gtgatatggc gtatcgcgtg gccgatcgca
     4141 tcggcgcttg tggcggtgcg cggcgcgcaa cgggacgcga cgatgccttc ccgatagtgc
     4201 ggatcgtctt ctgtgcggca gatgatgcgg tccatcatcc gaacggattc aatcgggaat
     4261 ccgccggccg ccgattcggc cgagagcatg acggcatcga caccgtcgta caccgccgtc
     4321 gcgacgtccg acgcctccgc gcgggtcggc accggtgtgc ccaccatcga ttccagcatt
     4381 tgcgtggcga tgatcaccgg ctttcccgca ttgcggcagg cacgtacgat ttgcttctgg
     4441 atggccggga cgcgctccgg cggaatctcc acccccagat cgccccgggc gaccatcacg
     4501 gcatcggccg cttccacgat gccttccaga tcgcgaatcg cggcgggctt ttccagcttt
     4561 gccagaatgc ccgctcgatc tttgacgatc cggcgaactt ccagaatatc ctcgacacgc
     4621 tgcacgaatg acagcgcgac ccagttcacg ccgagcgaca ggccgaattc cagatcacgc
     4681 cggtctttct ccgtgatggc cgccacgggc agtatgacgt cggggatgtt cacccccttg
     4741 cgctccgaaa gcacgcctcc ggtgatgacc gttgtttgcg cgaggtccgg gccacagcgt
     4801 tctaccttca actggatgcg gccatcgtcc agtaacagcg ccgtgcctgg ccgcagcgcc
     4861 gtgaagatct ccggatgagg caatgcgacc cgggtggcgt cgccaggcga ggccgtgtcg
     4921 aggtcgagcc ggaatgccgc gccgggttcg agcgtgatcg ggccgttggt aaacacgcct
     4981 atgcgcagct tcgggccttg caggtccaga agtatgccga tgggactccc cacatcccgc
     5041 tcgatggcgc ggatcgtgtc gtaccgttgc tggtggtccg cgtgggtgcc atggctcagg
     5101 ttgagacgga acacatcggc ccccgcgtgg tagagggctt caatggtttt tctgtcggag
     5161 ctcgatggtc cgagcgtggc aatgatcttt gcgttgcggc ggcgggccat tcgttattct
     5221 cctgtgcgcc cgatgtagat cgcgcggaaa tcgttgacgt tcgtgagcgt cggcccggtg
     5281 atgatcgagt cgcccagcgc ctggaagaac ccatgcccgt cgttgtcttc gagccggtgg
     5341 atcgggctga tgccgaggga gcgggcgtgt gcgagcgagt cgggacggag ccgcgctccg
     5401 gcgatgtcgg ctttcccgtc cgccccgtcg gtgtcacccg cgatcgagaa gatatcgggc
     5461 tcgccgctca gcgccaggcc aagcgaaagc aggaattcca cgttgcggcc gccacacccg
     5521 gcgcctgtga cggtgacggt tgtttcgcct cccgacagca gaacacaggg ggcgcggaaa
     5581 ggttgcccat ggcgacggac ctgccgtgcg ataccggcca tcaccacgcc gacatcacgt
     5641 gcttcccctt ctatcgaatc gcccagtatg taagcgtcca ccccacgttc gcgagcgacc
     5701 gcggccgccg cttcgagcgc catctggggg gttgcgatga catgggtgtc gatcgggggc
     5761 agccgggcgt cgccgggctt gagcgtttcc gtgagtccct tctcaagcat cgcgatcacg
     5821 gtggccggga caggaatctt gtagcgacgc aggatgtcca gtgcgtccga gcaacgggtc
     5881 ggatccggaa ccgtggggcc cgaggcgatg tcgctgacag tgtcggcggg tacgtcggag
     5941 atgatcaggt tcaccactct cgcgggatgg caggcggccg ccagatgccc gcccttgatc
     6001 gccgatagat gcctgcgcac gcagttcatc tccgtgatcg tcgcgccgct tgcgagcagg
     6061 gcctgattga tgtcctgctt ggtatccatg ccaatccctt cggcgggcaa gggcagcaga
     6121 gaggatcctc cgcccgatat cagacaaatc accaggtcat cggcgctgag tcccgagacc
     6181 atctcgagca tgcgcttggt ggcccggtgg ccgttggcgt cgggaatcgg gtgggccgcc
     6241 tcgacgatct cgatgcgggc gcagggaacg ccatacccat atctcgtgat cacgagtccc
     6301 gccagtggcg ctggccagtg gtcttccacg gctttggcca tcgccgccga cgccttcccg
     6361 gcaccaatca ccagggtccg gccccggggc ggggcgggca ggaacgcggg gatgcgccgt
     6421 gcgggctggg cggcggctat cgctgcctga aacatgtcat ccagcaagtg ctcgaccgag
     6481 ttctcgaccg tgggacaagg caataccgcc tcgtagggct cactcatggt ggtcctggtt
     6541 ttgttgggga atggatccgg cggtcaacgc acgcgccggg gtgcgttgac ccgccggtga
     6601 aatcgacgga aggttcgcct tttggtatca ggcggtcgcc gaagcgtttt cgacgagcgc
     6661 gcagacggca gcggtgactt ccgccgtcgt cgcccgcccc cctaggtcgc gggtatgaag
     6721 ctcgggccgg gccgtgacgg cctcgatcgc gtccatgacg cgcgcggccg cctgcgtctc
     6781 gcccagatgc tcgagcagca tgaccaccga ccagaacgtg ccgatcgggt tggccaggcc
     6841 cttgcccatg atgtcgaatg ccgatccgtg gattggctcg aacatggatg gatagcgccg
     6901 ttccgggtcg atgttgcccg ttggcgcgat gccgagactg ccggcaagcg cggcggcgag
     6961 gtcgctgagg atgtcggcgt gaagattcgt ggcgacgatg gtgtccaggg acgcgggccg
     7021 gttgaccatg cgcgccgtgg cggcgtcgac gagctccttg tcccatgtga catcggggaa
     7081 ttccagtgcg atcaacttgg cgacttcgtc ccacatgacc atggcgtgcc gctgcgcgtt
     7141 gctcttcgtg atgaccgtga gcagtttgcg cgggcgcgat tgcgcaaggc ggaaggcgaa
     7201 tcgcatgatg cgctccaccc cggcgcgcgt gaggatggac atgtcggtgg ccgcctcaag
     7261 gggatggccc tggtggaccc gcccacctat gcccgagtat tccccttcag agttttcacg
     7321 gacgatgacc cagtccaggt cctcggggcg gcaacgtttg agcggcgcgt cgatgcccgg
     7381 cagaatgcgt gtggggcgca cgttcgcata ctggtcgaac ccttggcaga tcttcagtct
     7441 caacccccac agcgtgatgt ggtcggggat gtccgggtca ccggcggagc cgaaaagaat
     7501 ggcgtccttg tcgcggatgt cgtccaggcc atcggccggc atcatcacgc cgtgctggcg
     7561 gtaatagtcg gcgccccatc cgaagttctc gaattcgaag gcgaattgtc cgctggccct
     7621 ggcaaggctg gccagcaatt gttggccggc gggaacgact tccttgccga ttccgtctcc
     7681 aggaattgtc gcgatacggt atgttttcat gcccacttcc tcaaagattc aacgttcggt
     7741 ccagatccag gttgtatttt ggcggaatgt cgagttataa atcatccaga aatacaaagc
     7801 atgattaact aaaattcaca aataagcatc caccggattg tggcgctggt ttcactgatt
     7861 gaagcgttgc gagcctttgt atcggaagtg ccatgacaga taccgttcaa cccgccgatt
     7921 ttgggttttt ctcgacgctt gtgtccctgg gcagcctgag cgcggcagcc agggagctgg
     7981 ggcttacgcc tgcggcggtc agcaagcggc tcacccagat ggaacagcgc acaggggttc
     8041 cgctggtcaa tcgaacgacc cgtcgcatga tgctcacgcc cgaaggcgag atctacctgg
     8101 aatatgcccg ccgcgtcctg gacgaggtgg atgcgctgag cgaactgctg ggaggcgcga
     8161 aaacgcgtcc gatggggttg ttgcgggtca acgcgacact gggtttcggg cgtaatcatg
     8221 tggcgccggt catttcccgg tttgtatcgg cgtacccaca ggtctccgtc caactgcagc
     8281 tctctgtgac gcccccgccg ctgaccgccg atgtctacga cgtgtgtgtg tgttttggcg
     8341 aaccacccga tgcgcgcgtg atagcgcgaa ggctcgcgcc gaaccggcgg ctgctatgtg
     8401 ccgcacctgg ctatcttgca cggcatggta tacccctcac accgcgggat ttgaccaagc
     8461 accattgcat cagtattcgt caaggcgacg aggcgtatgg tgtctggcgc ctgaggaacg
     8521 ggcgggaggc gttggcgaaa tcggaggcga tccgcattca cggccccctg acgaccaacg
     8581 acggagagat cgccgtcaaa tgggcgcttg atggccacgg cattttgatg cgggccgaat
     8641 gggacatcaa agactatttg gccgacggca ggctggtgac ggtgttgccg gattacgcca
     8701 cgccagcggc ggatatttac gcggtctatg cgcaacggca tcagttttca acgcggatac
     8761 gcgcctttgt gggctttctg gccgacgcgc tcttcccacg gcagcggtag gcgcacgaaa
     8821 acgtcgggcg tcagcgtggc gtccagatct tcaatatgct cgaaaccgac ggagacttcc
     8881 gcgtagatga tggggttgat gcacagttcg ccctgcgcgg cgctcgcatc cagctgcgca
     8941 gccgaccacg cgccccactg cgggtcgtcg gtgagcacgt ccagcaggac gttggagtcg
     9001 accaatacgc gactcatgcg ccgcgcgtca gttgcatgat ttcgtcggtg ctcaggcgtg
     9061 tggtggcgcg gccgcgcaag cgtgccgcca cagccggtcc cgctgctccg tgatccagcg
     9121 ccacgagcgg gccgaggggg ctcaatgcgg ttggaacggt gtcatcaact gtgtctgatg
     9181 aatgctcatc ccccaataat ggggtagagc ccgctgttga ctaggaagcc gcatgaatac
     9241 tggctttccg agaggaagaa actggtgccc cccccgtgag tcgaacacgg caccaacgga
     9301 ttatgagtcc gctgctctaa ccaagcatga gctagagggg cacgtacagc gatagccgat
     9361 gattactaca gtactccgac cgcaagccgg tgattatcgc ataaagtgat tctcgtcgaa
     9421 gggcgtggcg ccaccccttg cctgctggtg cgaaatgtga ctttaatggg atctgccgga
     9481 tgatccggtt ctggttttat cccattgaag ccatcggagg tttcgccatg acggacgatc
     9541 gcgtaatcac tctcgggaaa ctctcgatcc gttacttgat cgatgggtcg caaaccaaaa
     9601 cgatgggcat gttcgagctc accgttcagc ctggcgccaa tgtcccgccg ccgcacagtc
     9661 acgccaataa cgaggaaatc gtttatgtcc tggcgggcaa gctgcgttac agggtgggtg
     9721 acgatacccg tgatcttcag ccaggcgggt caatgcatac ccccaaaggg gtggtgcatg
     9781 ctttcgccaa tccgttctcc gacgtggccc atgcattgat cgtgctgtca ccggatatcg
     9841 gcgcgcaata cttccgtgac atcggcgagg cgattggagc gggtggcccc ccggacaagg
     9901 cggcacttat ggcggtgatg aaacggtatg gtcttgttcc ggccttgccg ccagccgctt
     9961 cttgacggca catcagtcac aaattgcaag tagatatgtg tttgtacgcg aattcagtgc
    10021 gtgctcattt acgccatata caatctcgcc tgtttataca tatgatcttc tcgcgacccg
    10081 cggcatcagg ctgaaataac tattcattga atattcatgc aatgccgatg cgcgcaaggt
    10141 gaggggacgg acgctttgcc gcgaatagcc cggccacaag ttgggttcca acgcggcgct
    10201 gcagactgca taggaagact ttcatggctc gtttcgcaac gctggtcctg ttgtggttgg
    10261 tgggggtggc gccgtcctgg gcggcgagag tggcgcaggt atctccccag gggcgcgtca
    10321 gtgttgccga gagtgtcaag atcacgttcg attcgccggt catccatttt ggcgatggcc
    10381 aggcccacgc gccggcgacg gtcaaatgcg atgatcccgc actgaagggt agcggccgct
    10441 ggctggacgc tgagcgttgg acgtacgtct tccagtcccg gccagggccg ggggtgacct
    10501 gccatgtcga gatcgatccg gcattccgcg cgctggacgg caagccggtc gaaggagcca
    10561 cgcatttcca gttcgacacg ggggcgccga ccatccgcac ggtgcgtccg tatggctcgt
    10621 cgcagatcga cgaagaacag atgttcgtgc tggagttcac cggcgcggtg aacaaggatt
    10681 cattgctcgc gcacgcgtcg tgcctggcgg aggggatcgg ggaggccatc ccggtgcggc
    10741 tgataaccgg caccgagcgc gaaaatgtgc tcaaggccgc ggtgtacgac aaggagaatc
    10801 gcgggccggg ttcactggtg ctgcaatgca agcggcggct cccggccaat gcgtcggtgc
    10861 agttgcgcgt ggggccgggg gtcgcgtccc cgtcgggcat cgcctcgacc cagcccgcca
    10921 tcttcaagta caaggtgcgg ccgccgttca ccgtgagttt cagttgccag cgcgagaatg
    10981 cccatgcgcc gtgtacgccg gtgctgccga tatcgctcag tttcagtgcg ccgatcccgg
    11041 ccgaccaggc ggcgaagatc cggctgaaaa ccggcaaggg cgaacgctct cccgacaaag
    11101 gcgattacgg atatcgaaac agtgtggaag tggtgcggtt caaggggcca ttcccgcccg
    11161 atgcggatgt gaccctcgtc ctgccgccgg acttgaagga tgattccggc cggccgctgt
    11221 ccaacgcgga ccagtttccg ctgaccacgc acatggcgtc atttccgccg ctggtgaagt
    11281 tcgccaaacc gaccttcggt gtcatcgagc gcttcgccaa cgtgccgtcg gggggaagcg
    11341 aagccgacta cccacccagc gtaccgctga cggtgcgcaa cgtggaggcc gatttaagaa
    11401 cccgcgatct gctggtgtcg tcgggcgaga tcaaggacta cgtggtgcgc gacgacgccg
    11461 atgtcctgca ttggtacgaa cggctgataa aactgaatga ggcgtatctc acgcagcggc
    11521 agatcgatga ggtcatgcac gatcgcccgg tgcctgagaa caaaaagcgc ggcgaagccg
    11581 tcatcgacac gcgcggtgtt tctgtattga atggccagtc tggggggcgt cagttgaagc
    11641 tgcccgcgcc gccgaagagc ggcacccggc ctttcgaagt gattggtgtg ccagttcccc
    11701 gactgggttt tcatgtcctg gaagtcgaat ccccgcgcct gggccagtcg ttgcttggcg
    11761 aggacaagcc catgtacgtg cgctcgtcgg cgctggtcac caacctgggc gtgcacatca
    11821 agcaagggcg cgacgatctg ctggcctggg tcacgacgct ggaccagggc aaggtcgtgc
    11881 ccggtgccgc catcacggtg ctgagttgca agggtactgt gctgtacaaa ggcaagacgg
    11941 atgcgcgcgg catctggcac aaacgcggcg tggacgatat cccgcaatgc gacagcggct
    12001 tcaacggcat ctacgtatcg gcccgcatca cgaaggataa tcccgtcgcc cagggcaagg
    12061 ccgatttcgc cttcgccttg tccacctgga acgacggcat cgagccctgg cgtttcaacg
    12121 tgcccaccga caacagtcct tatcgcacga cattggtgga cacggtctat gaccggccgc
    12181 tgttccatgt gggcgagacc gtctcgatga agcatttcct gcgggtggag acgcgtgacg
    12241 ggttggccgt gccgaccgac tccgatcctg ggccggatcg catcgtcatt tcgctcgagg
    12301 ggtctgacca gcgctacacc cagcacctgg attggaaggc cacgccaacc ggtggcctga
    12361 gcgccgtgag ccagttcgtg attcccaagt cggcgaaggt cggcacctat tcagtcacct
    12421 atcaggacgc gaacggcact tggttcgggt cgagctcgtt ccgggtggcg gcgttcaagc
    12481 tgccggtgta tcaaggcacg ctcgaggtca gcgacaaggc ggggtcgcat acgttgatcg
    12541 cgccgacatc cgtgcatgcc gatgtgcaga tgtcgttcgt ttccggtggc ccggcgggca
    12601 agctgcctgt gacgctgtcg ggtgtggtgc aggagaattg gctgaatttc gatggctacg
    12661 acgattattc gttcaatgtg ccgctaagca ccaatccgga tctggacagc gattattcag
    12721 gggccgatcc agccgacacc gccacgcacg acaggcaggc gcttttccta gacaagaaac
    12781 cggtgacgct cgatgcgcac ggcggcgcgc gcatcgtgtt ggactcattg cccaaggtga
    12841 cgcgtccgca gaattggctg ttcgaagcca gctatcccga ccccaatggc gaaatccaga
    12901 cgctcagcca tacggtggcg gtgtggccgg ctgcggtggt ggtcgggctg cgtacgggta
    12961 gctgggtgca gaaaggcaag gccacgccga tcagcgtggt ggccctgtca cccgcgggcg
    13021 tgccgcaggc tggcgtgccg gtcagcgtcg atgcgcggca gaagaccacg tactccacgc
    13081 ggcgacgcat ggtgggtggg ttctacagct acgacaatca cgttgtggtc aagagcatgg
    13141 gcaccgtctg ccagggcaag acggacagcc acggcaagct cgagtgctcc atcaccctca
    13201 cgcattccgg ctccatccaa ttgctggcgc atgcgaagga cgcacaaggt cggcaggcat
    13261 ccgcgcagaa cacgttgtgg gtcatcggtg gcgacgattt atggttcggt ggatcgaacg
    13321 atgatcgcat ggatgtgatc cccgagaaga aaatctacaa gcctggtgaa accgccagct
    13381 tccaggtacg catgccgttc cacgaagcga ccgcgctggt ggcgatcgaa cgcgaagggg
    13441 tgttgtcggc gcgggtggtg catctcgaag gtgacaaccc caccgtcaag attccggtgc
    13501 aggccaagtg gggccccaat gtctacgtgt cggtgctggc attgcgtggc cgtctgcgcg
    13561 aggtaccctg gtattccttc ttttcctggg gttggcgcca tccggcggat tggtatgacg
    13621 cctacaacaa cgagaacaag gagttcaagg cgccgaccgc gttcgtcgat ctgtccaagc
    13681 cgtcgttccg ctttggcctg acacagatca aggtgtcgga tcaacgtgac gccttgaatg
    13741 tgaaggtcac ggccgacaag ccggtatatc aggtgcgtgg caaggccaac gtcacgatac
    13801 acgtcaccaa gccggatggc acgccggccg ccaatggcca agtggccttc gcggcagtgg
    13861 acgaggcgtt gctgcaactc gcacccaaca acagttggga tctgctcggc gcgatgcgcc
    13921 agtcgcgcag ctatggggtg cgcacagcca ccgccgagat gcaggtggtg gggcgccgcc
    13981 actacggccg caaggcggtg ccggccggcg ggggcggagg caagagcccg acccgcgagc
    14041 tgttgaacac gctgttgctg tggaaaccca tggttcagct cgatgccaac ggcgaggcgc
    14101 atctcgctgt gccgctcaac gattcgatca cgcgcttccg tctggtggcc atcgccgact
    14161 acggtcaaga gcgtttcgga acgggtagca ccgacatccg ggtgacccag gacctgcagg
    14221 ccatatccgg cctgccgccc ctggtgcgcg agggtgatgc ctaccaggcc atggtgacgg
    14281 tgcgcaatgc cacgacgcgc gacatgaagg tcgatgtggg cctgggttac agcgggaagg
    14341 gtgtgccagg ggagacgctg ccgtcgaagg tggtggacgt ggcggccgga accgcgcaag
    14401 gggtgacttg gcgggtcaag atgcccgaag ccgatctacc agacgaccag acccggctcg
    14461 attggcgcat acaggcgcgc gagcagggca ccggccacgc ggctgatgac ttggtcgtgc
    14521 atcagcagtt gatacccagt gtccccgtgg ccgtcacaca ggccgccttg ataggggtgg
    14581 atgcggcctc caggccattg gagctgccgg ttcaggcgcc ggacaaggcg ctggttggca
    14641 ccaacggccg cccgcgcggc gggctgacgg tgacgatgcg ttcgtccttg gccggtggtt
    14701 tgccgggggt caggcattgg ctggcggttt atccgttctc gtgcctggaa caacgcacgt
    14761 ccaaggccat cggcatgcgt gatccggcgc aatggcgcga cttgatcagc cgtctgccga
    14821 actatctgga caacgatggc ctggcctcgt actttccggg cgccacgcat ggcagtgaag
    14881 tgctcacggc gtacgtgctg gaagccagtg acgaggccag cgcattgggg atgccgttca
    14941 cgctgcctga cgcggacaag cgcaagatgc tggatggcct gcgcgcctac gcgcagggcc
    15001 gcatcgtgcg gcaccattgg gcgccacaga aggatctgga cgtgcgcaag ttgttggtga
    15061 tcgaagcgtt gtcgcatgat cgcgaagcca ccgtccgcat gctcgacagc atccagatca
    15121 cgcccaatcg ttggccaacg tcggcggtta tcaactggct gtcgctgttg caacgcatgc
    15181 ccgatattcc caaccggacg gcgcaactgg ccaaggccag gcagatcatt cttgggcgca
    15241 tgctcagtcg gggccagtcg ttggtgtttc ccgacgatga cctcaacaac tggtggtggc
    15301 tcatggacaa tcgcgcggtc aattccgcga ggctgttgct gacggtcgct g
//
LOCUS       DASTNK010000106        34631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_75999, whole genome shotgun sequence.
ACCESSION   DASTNK010000106 DASTNK010000000
VERSION     DASTNK010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 34631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34631
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75999"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VFR20_07895"
     CDS             <1..510
                     /locus_tag="VFR20_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0230478.1"
                     /translation="RDVAVEFFTMSKSYNMAGWRVGFMVGNAELVNALARIKSYHDYG
                     TFTPIQVAAIAALEGPQDCVAKVVEQYRNRRDVLARGLRDAGWNVDVPKASMYIWARI
                     PEPYQSLGSLEFSKRLLADAKVAVSPGIGFGDYGDGYVRFALIENEQRTRQAVRGIRD
                     MFRKDGLIK"
     gene            507..1811
                     /locus_tag="VFR20_07900"
     CDS             507..1811
                     /locus_tag="VFR20_07900"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU0230479.1"
                     /translation="MSVVKVGLLGFGVVGAGTWEVLTRNAEEIARRAGRRIEVVAVSV
                     RNVDKARRVVGDHVMVTTNGLDVVSHPDIDIVVELVGGDTVAREWVMEAIRRGKHVVT
                     ANKALLAKHGNEIFSQARAQSVMVAFEAAVAGGIPIVKAIREGLTANRIQWVAGIING
                     TTNFILTEMRARGLSFGEALAEAQRLGYAEADPTFDIEGVDAAHKLSLLASLAFGIPV
                     QFDKVYVEGISKLAQEDIAHAERLGYRIKLLGITRRRPEGIELRVHPTLIPSGRLLAN
                     VEGAMNAVLVSGDMVGPTLYYGQGAGALPTASAVVADLVDVARLDSADPDHRVPYLAF
                     QPDALDDTPVLPIAEVLSSYYLRLRVEDRPGVLADLSRILSDAGISIGSMFQEPHGET
                     DADIIFLSHEAREGAVDAAIERIRDLPFVKSGVTRLRVEHLL"
     gene            1808..3226
                     /gene="thrC"
                     /locus_tag="VFR20_07905"
     CDS             1808..3226
                     /gene="thrC"
                     /locus_tag="VFR20_07905"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU0230480.1"
                     /translation="MKYRSTRGGMAPQSFSDILLEGLAPDGGLAVPVSLPRVERATLE
                     SWRPLAYADLATAVLGLFIDDIPTADLSRLTHAAYTPRLFGSTEIVPLKPLDGGLMLL
                     GLSQGPTLAFKDMAMQFLGQVFEYVLGKRGATLNILGATSGDTGSAAEYALRGKHGVS
                     VFMLSPHGRMSDFQRAQMYSLQDANIHNIAIEGVFDECQDIVKALAGDLAFKSRHHLG
                     AVNSINWARIAAQVVYYFWGWLRATDALDATGKREVSFAVPSGNFGNILAGHFARAMG
                     LPVRRLVLATNENNVLEEFFRTGVYHPRPAAQTYATSSPSMDISRASNFERFVFDLVG
                     HDAQRVSDLWRRLGETGAFDLSDLRPEFESRYGFVSGVSTHADRLSTIRTVNERTNVL
                     VDPHTADGIKVAREHVEEGVPMLVLETALPAKFDATIREAVGHPAPVPDHLQGLARLP
                     QRVRVMPCDAGLVREYIARHAI"
     gene            complement(3254..3679)
                     /locus_tag="VFR20_07910"
     CDS             complement(3254..3679)
                     /locus_tag="VFR20_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEU0230481.1"
                     /translation="MDALDHALISLLRKNARLTVATLAKKLDVSRGTISNRIARLEND
                     GVIVGYTVRLRPDAQPNEIRAWMSIAIEGNETRIVIASLLGEPGVAALHDTNGRWDLL
                     AELRATNLADLSQVLERIRLIRGISNTETSFHLKTYRAS"
     gene            3864..4982
                     /locus_tag="VFR20_07915"
     CDS             3864..4982
                     /locus_tag="VFR20_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HEU0230482.1"
                     /translation="MTTRTKAVNDARAATLYLDVADIATIFRSEGAAAVFSRLGDYIR
                     NDYLRWDDFDKSPRVAKYSRLGVIELMPIADDTTYSFKCVNGHPRNIERGLPTVMGFG
                     ALADVDTGAVRLISELTLTTALRTAATSALFASVLARPDSRSMALIGNGAQSDFQALA
                     FHHLLGIEEIRLFDIDDAATDKLLRNLHSTDIRFKVCDSVAQAVQGADIVTTATAEDA
                     HAQVLRAGMIEPGMHLNAVGGDCPGKTELDPEILHMGPVFVEYEPQTRVEGESQNMPG
                     DFVVTPFWKVLRGLEPGRTSPGQITIFDSVGFALEDYSALRYVRDMACKLGLGHTLAL
                     VPRLDDPKNVFSLTEARNVREFCGQGVTQPCEKEAMLP"
     gene            4955..5875
                     /gene="rocF"
                     /locus_tag="VFR20_07920"
     CDS             4955..5875
                     /gene="rocF"
                     /locus_tag="VFR20_07920"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEU0230483.1"
                     /translation="MREGSNVAVSLIGAPTDIGAGCRGASMGPEALRVAGITEALARL
                     SIPVRDCGNLSGPLNPDAPMREGYRHFNEVVRWNRLVHDAVHAELADGRLPLMLGGDH
                     CLGVGSVSAVARYCREQGRVLKVLWLDAHADFNTATITPSGNMHGMPAACLCGYGPSE
                     LTRLGGEPPILPPEALRQIGLRSVDAQEKRLIRDVGIEVYDMRAIDEQGMRQIMRAAL
                     RDVDGDVHLHVSFDVDFLDPEIAPGVGTTVRGGPGYREAQLCMEMIADTGRMGSLDIV
                     ELNPALDVRNETAELAVDLVGSLFGKRTLA"
     gene            complement(5911..6381)
                     /locus_tag="VFR20_07925"
     CDS             complement(5911..6381)
                     /locus_tag="VFR20_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU0230484.1"
                     /translation="MAEYAAEIIWVRNDEDFLDKKYSRRHILRFDGGAQITASASPHM
                     VPVPLSDSTAVDPEELFVSALSSCHMLWFLAMAAKQGFRVDRYVDKAVGVMGENRDGD
                     WAMIRVTLRPEVIFSGDKQPTHNQINEMHEQAHEECFIASSVKTDVICEPVHSE"
     gene            complement(6575..7438)
                     /gene="hpnD"
                     /locus_tag="VFR20_07930"
     CDS             complement(6575..7438)
                     /gene="hpnD"
                     /locus_tag="VFR20_07930"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEU0230485.1"
                     /translation="MSPDEYCQDKAARSGSSFYYAFLFLPPDRRRAITALHAFCREVK
                     DAVDETTESSVARIKLAWWRTQVDRLFTGSTEHPVMTALASHVQPYGITAERLHAVID
                     GEEMNLDQPGYPDWPSLREYCWHAAGVVGEMSAGIFGYTDPATLMYAEKLGLALQLTN
                     IIRDVGDDARRGRIYLPTDDMDRFGVAAADIMNRRHTDAFVALMRFETQRARDCYRDA
                     VRALPEADRRAQRPGLMLASIHHTLLGELERDQWHVLDQRVALTPIRKFWLAWKGWVG
                     GGRGTVRRLGG"
     gene            7717..8847
                     /gene="alr"
                     /locus_tag="VFR20_07935"
     CDS             7717..8847
                     /gene="alr"
                     /locus_tag="VFR20_07935"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU0230486.1"
                     /translation="MPRPILATISLPSLTHNLAVVAGRLNDARPAGRMAPFIWAVIKA
                     NAYGHGILNGMRAFSGADGLAMLDLDEAVCCREAGWSGPILLLEGGFEPSDLSVIDRH
                     RLTVTVHSQAQLDMLRGAAPQAPIDAMIKLDSGMGRLGFPPDAYRDAYRQAHDLKQRG
                     ILGNLGKMTHFARADDDPGVTRAQLRVFDEVTGGLPGRISVCNSAATLTPGLWSGLPE
                     PYEQWVRPGICLYGASPFADRPASSFGLRPAMTLSAELISVRRIPAGYSIGYGHRFTA
                     SDAMTIGVVSCGYADGYPRHAPTGTPIVVAGVATRVLGRVSMDMLMVDLAPVPDARVG
                     LPVVLWGEGGPSVDDVAAACGTIGYELLCAVAPRVPRKVVSG"
     gene            8879..9586
                     /locus_tag="VFR20_07940"
     CDS             8879..9586
                     /locus_tag="VFR20_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0230487.1"
                     /translation="MKDKCVLVTGATKGIGWAISRRLADQGCHVVGLARHTQDIDFPG
                     YLYSCDLANAGETEETLRVIREKYPVDAVVNNVGIVMPQPLGEVELTALYGVLDLNVR
                     VAVQVVQTFVESMKARREGRIVNLCSRATGGALNRTSYSAAKSALIGCTRTWALELAE
                     YGITVNAVSPGPVETELFRANHPVGSESEAQALASIPMKRLGQPADVAAAVVFLLSDE
                     AGYITGQVLGVDGGGSL"
     gene            9665..11017
                     /gene="radA"
                     /locus_tag="VFR20_07945"
     CDS             9665..11017
                     /gene="radA"
                     /locus_tag="VFR20_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU0230488.1"
                     /translation="MAKTKSSYVCNTCGASYPKWEGRCAQCGEWNSLQETLAPGADAH
                     RFASLAGTAPVQRLADIEAREIPRQPTGLAEFDRVLGDGLVDGGVVLIGGDPGIGKST
                     LLLQAMASLSRTTGVLYITGEESPEQVALRAQRLEIAGTGVDLLAEIRLEAIIDTLTE
                     RRPGVAVIDSIQTLYSSALSAAPGSVSQVRECAAQLTRLAKQTGITIIMIGHVTKDGS
                     LAGPRVLEHIVDTVLYFEGDTHSSYRLVRTFKNRFGAANELGVFAMTDHGLRGVSNPS
                     ALFLSRHDQDVAGSCVMATQEGTRPLLVEIQALVDGAHVPNPRRLSVGLESTRLSMLL
                     AVLHRHAGVATFDQDVFVNAVGGVKIVEPAADLPVLLAIISSLRNRPLPKGLVAFGEV
                     GLAGEIRPAPRGQERLREAAKLGFSVALIPKANAPRQPIEGLEIWAVDRVDAALARVA
                     "
     gene            11077..11853
                     /locus_tag="VFR20_07950"
     CDS             11077..11853
                     /locus_tag="VFR20_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU0230489.1"
                     /translation="MEWYVIVPACLAAGAAIGFVGGVLGIGGGLIAIPMLGIILNMTQ
                     QQAQGTALIMVLPAVLMAVRKYNQRARIDLRAAAAGAAASIVFTWVGAKIALGVDPIL
                     LRRVYAAFVLCIAIFYFYKGMGRHAKASQPSSVRSASEFHKGWFALVGVLAGLIGGVF
                     GVGGSVMAVPILTTIFRLPQTGAQALALTMIIPGSTVALITYATHGQSDWLVGLPLAL
                     GSIFIVPHGVRFAYALPEPRLKVVFACMLLVITALLLAKA"
     gene            11872..12591
                     /locus_tag="VFR20_07955"
     CDS             11872..12591
                     /locus_tag="VFR20_07955"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HEU0230490.1"
                     /translation="MVSAPPTTMALASGAMCFDPALVRAPGAFFFDPTESRLSAEPVS
                     TGGRQAAWFVTGEFGEGVLRHYQRGGMIARLSRSRYLWAGASQTRSFAEFRLMHAMRE
                     HGLAVPRPIAAAYWRHGPTYTAAIIVERIAGAVPLACAMDPVSCPKVARAIFDMHEAG
                     FWHADLNAFNILFDGGGQVWLIDFDRGRRCRLTSRRRAANLARLRRSLIKVAGAGGER
                     YWVGLDVAYRRLVMGKPGDEA"
     gene            12670..13023
                     /locus_tag="VFR20_07960"
     CDS             12670..13023
                     /locus_tag="VFR20_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEU0230491.1"
                     /translation="MMIRVFGLNKCDTCRKARAWLDEHGVAYDFIDYRDNPVPASTLA
                     QWASAVGGWDKLVNRASLTWRGLPPERKSPESPAQWLALIAEHPTLVRRPVLTRLGED
                     PITGFSDKKYAEIFK"
     gene            13066..13752
                     /gene="rpiA"
                     /locus_tag="VFR20_07965"
     CDS             13066..13752
                     /gene="rpiA"
                     /locus_tag="VFR20_07965"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU0230492.1"
                     /translation="MLTQQELKQNVARAALDYVIPSLDPNVVLGIGTGSTVDLFIDAL
                     AAHKNRFRAVVSSSERSAARLRGHGITVLDLNEVASMPLYVDGADEIDDALHMTKGGG
                     GALTREKIVASVAERFLCIVDETKCVRRLGRFPLPVEVVPMALRVVARRLEKLGGAPR
                     ERDGFVTDNGCRILDVSGLDIADPANLEATINDIPGVVTCGLFAIAGADLALVSTQDG
                     VRELTPGRSQ"
     gene            13815..14537
                     /gene="phoU"
                     /locus_tag="VFR20_07970"
     CDS             13815..14537
                     /gene="phoU"
                     /locus_tag="VFR20_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU0230493.1"
                     /translation="MLEHTNKQFHTDLEETRSRFLQMGGLVESMVRDAMVVLATGDQA
                     IVDRVREHEKAVNKHEVEIDERIGLLIARHQPTAGDLRLLLSISKMLTDMERCGDEAE
                     KIAKVARRLYEADSRYTPAVDLAHIADCVTTMIRDALDSFAREDPLHAAEVVRRDKSV
                     DKEWKASLRQMITYMIEDPRTISRSIDLVFIARSLERIGDHAKNMSERVIYMVRGDDV
                     RHTGVKNAERTARGEAGSDDGV"
     gene            complement(14548..14820)
                     /locus_tag="VFR20_07975"
     CDS             complement(14548..14820)
                     /locus_tag="VFR20_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018025743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEU0230494.1"
                     /translation="MARMIQCAKLKREAEGLDFPPYPGELGKRIWQHISKEAWTQWIS
                     TQTRLVNENHLNLADPDARKFLAGQMERFLFEDGHVEAQGFVPPSR"
     gene            complement(14899..16251)
                     /gene="argA"
                     /locus_tag="VFR20_07980"
     CDS             complement(14899..16251)
                     /gene="argA"
                     /locus_tag="VFR20_07980"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEU0230495.1"
                     /translation="MATTDDTFSAQDAPEYAPAQFVRWFRDVAPYVHDFRGKTFVIAF
                     GGELVGDGALNALVQDLSLLSAVGVRLILVHGSRPQVNEQLRLKGYDTQFGRGTEPTS
                     AEALECAKEAAGEIRLDIEAAFSQGLPNTPMSHAQIRVISGNFVTARPVGVIDGVDYK
                     HAGAVRKVDLEAIKNIIAHGAIVLLSPLGFSPTGEAFNLAMEDLATSAAVALRAEKLI
                     FLTQDRTVLNSDGTTDTELARKDADDLLASNTLDEDTASFLSHASLAVKRGVARAHLV
                     PYTLDGSVLLEIFTHDGVGTMVVEDTLDDLRPATVDDVGAIVQLIEPLEEDGTLVPRG
                     RAVIERDVERFTVLEHDGVIYGCVSLMPYPNDGMAEMACLIVHPEWQGTGEGEILLRH
                     TESQARAQGMKRLFVLTTRTSHWFLKRGFVQGGVSDLPHEKRAHYNHSRNSLIFIKRL
                     "
     gene            16335..20168
                     /gene="hrpA"
                     /locus_tag="VFR20_07985"
     CDS             16335..20168
                     /gene="hrpA"
                     /locus_tag="VFR20_07985"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEU0230496.1"
                     /translation="MQEPAGLSDNVHTRPGAAQPVVRRPPLHIDYPEDLPVSARRDEI
                     ARAIRENQVVIVCGETGSGKTTQIPKICLELGRGAERMIGHTQPRRLAATSVARRLAE
                     ELKTGLGDWVGYQIRFNDRSGPGTAVKLMTDGILLAETQRDPLLRRYDTLIIDEAHER
                     SLNIDFLLGFLLRLLPRRPDLKLIITSATIDAERFAQHYSINGRPAPVIEVSGRLYPV
                     EMRYRAVQDDGRKGAGNDAASGDEERDLIDAVVDGVAECAALGAGDVLVFLPGEREIR
                     EAAEALRKSHPIGAEILPLYARLSQAEQERIFHPSGNARRIVLATNVAETSLTVPGIR
                     YVVDTGLARVKRYSWRNKVEQLRIESISQASANQRAGRCGRIGPGMCLRLYDEADFAG
                     RARFTDPEVLRSSLASVILRMKALGLDDIESFPFVDPPSGRAIADGYHLLQELGALDD
                     ANRLTDTGRVLSRLPVDPRLGRMILAGREWQCLDEVLIIASALSVQDPRDRPMTQREA
                     AETAHAKFRDEKSEFLSFVKLWRWYGEQVAHKASQRKLVALLRQNFLSPLRLREWHDV
                     HGQLSSLVGEQGWRINASPATYEQAHMALLAGLLGNIGLKSEEGAGYLGARGIRFLIH
                     PGSPLQKKAGRWLLAGELVETSRLYARCVARIDPVWVERAAGNLLQRSWSDPRWDRKA
                     GQVVANERATLYGLPIYAGRRVHYGRVDPVRAREVFIRDALVTGDIETSLPFVVQNRK
                     LIAAIEKLEHRARRPDILVDDSLIQAFYDRQIPADIFQTASLEKWARRLGDDQARALL
                     LTRDDLMRHEAAGLTTDVFPKMMRWHGVEMALDYHFEPGSLRDGVTLTVPLFALNTVD
                     EPRSEWLVPGMLKEKVLLLLKSLPQKLRRHCVPLPDYAAAFHERWFERAADPGLGLFD
                     ALAQDIWEHCKVRVARTDFKMEALPPHLLMNFRVVDEHGRMLSAGRNLDQLKAEHARE
                     AQASFQKVAATDADVVRVLDHEHLTGWTFGALPEIMEIRRGGQSLVGYPALVDRGADC
                     DLDVFDDPSEARRMHRAGLLRLFRLALREQLRFQEKNLGDLTRLGMLYMGLGSQDELR
                     DQIIECSLAQACLADPWPVDAETFNQRCAEGKTRFGLVAQEVARLAGDVLTEWSALQK
                     KLPQARPWKASHADLLAQTDALMARMFIRDTPYDQLKQFPRYMKAARLRVDKLKADAQ
                     RDSRLMADMSGLVAQYQRARSALKGAPDARLDEFRWLLEELRVALFAQELRTPMPVSV
                     KRLQKAWAGLGTIAA"
     gene            20165..23653
                     /gene="dnaE"
                     /locus_tag="VFR20_07990"
     CDS             20165..23653
                     /gene="dnaE"
                     /locus_tag="VFR20_07990"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU0230497.1"
                     /translation="MTDTSPASPFVHLRVHSEFSVVDGIVRIPELVSRTVEFGQPAVA
                     LTDLSNVFGLVKLYKAARKAGVKPIAGCDVYVQNDADRDKPFRLLLLAIDRNGYLELC
                     RLLTQAWLDNQYRGRAEVRREWFKEVDGLIALSGARAGDVGHALEAGRAAEARSLAQA
                     WARLFPNRYYVELQRAGMDGDETYVQAAVRLAASLDLPVVATHPVQFLDRTDFRAHEA
                     RVCIAEGEQLANPKRTRRFTTEQYFTSSSDMAERFADIPSALANTVEIARRCSLTLDL
                     GTPRLPNFPTPEGISLDDYMVGLAEEGLEKRMVQLFPDEAVRREKYPAYRERLELECK
                     TITGMGFPGYFLIVADFINWGKNNGVPVGPGRGSGAGSLVAFSLGITDLDPIRYDLLF
                     ERFLNPERVSMPDFDVDFCQDNRERVIDYVKHKYGRDAVSQIATFGTLGAKAVVRDVG
                     RVLEMPYSVCDGLSKLIPFSPTDPWTLDRALSDEPAFKERYDQDEEVHALVDLARPLE
                     GLTRNIGMHAGGVLIAPGKLTDFCPLYCQGMDGSAVSQFDKDDVEAVGLVKFDFLGLR
                     NLTILDWAVRYVRAFNPDMRDFDLMSLPLDDAGTYKLLSDGNTTAVFQLESRGMKELL
                     GRLRPNTFEDIIAMLALYRPGPLESGMVIDFVDRKHGRAAVDYFHPDLESVLKSTYGV
                     IVYQEQVMLISQIIGGYSLGGADLLRRAMGKKKAEEMAKHRAIFEEGAVKKGYDADLA
                     VKLFDLMEKFAGYGFNKSHSAAYALIAYQTAWLKAYHPAEFLAATISSDLDDTDKVQV
                     FWKDALANGVAVLPPDVNASNYRFEPVADAHTEQGLPPRTMRYGLGAVKGTGQGAVEE
                     IIRARGEGGPFTGLFDFCRRVDRHTVNRRTIEALIRAGAFDNIEDNRAALLEAVGSAI
                     EAAEQAERSANQVSLFADDAGDIVADELASVTPWDLQTRLTQEKSALGFFFSGHLFDA
                     WRDEVRRFAPKPLSRLTPGRGLQWFAGVLAAVRVKMTRRGKMLYATLDDGSAQVEVAI
                     FNELYEQHRARLREDRLIIVQGKVSTDDFSGGLRVSAEAVFDLQLAREARARSLRIPL
                     NGNADAARLRKLLNPYRAEPENGVPGVPVEIVVHRGGFRCAVRLSDDWRVRMADAMLD
                     QLNGWTAPDEVEITYS"
     gene            23650..24750
                     /locus_tag="VFR20_07995"
     CDS             23650..24750
                     /locus_tag="VFR20_07995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0230498.1"
                     /translation="MRPLRVLHSEAATSFGGQEHRIYKEMLAMRERGHHLELVCQPDA
                     LLVERVRAEGFTVHTTPMNGHVNFIKSVAKIRRVLRKSRFDVLNTHSRRDTLIAGVAG
                     RLAGTPLIVRTRHLAIKAGSLLSYTWIPHRVTTVSDYVRINLIKAGVPPGRIATVYSP
                     IELPPPVEHSTLRDELGLAVDDVVVGCVAVMREKKGHMALLEAIEPLFNARPALHLVL
                     VGSGSPVFERVQAYVKEHGLARRVHLLGTRRDVPNLLAGCDLFALATEQEASGTVFVE
                     AAAAGLPVIGTDVGGVSEMLREGETGWLVPVGDTQALRAALERLVDDRALRKRMGEAG
                     RRLFRDEGKFSLETLAQNTERCYVEWLRERRA"
     gene            24747..25592
                     /locus_tag="VFR20_08000"
     CDS             24747..25592
                     /locus_tag="VFR20_08000"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0230499.1"
                     /translation="MSARNRTVPVLMYHHISPVDGMITTSPVNFELQLAWLARHGYRS
                     MSSAEFAAHLGGQAAPPRSILITFDDGYLDNWVYAYPLLRKYGFSAMIFLITSLIGDG
                     PARPHLGQGGLPETPSHRDCGQRIQAGDADSVMLRWNEVLAMRDSGVVEFHSHTHTHT
                     RWDKSPRAAEKNQLMTRELMDSRATLEARLGSVSDHFCWPQGYFDADYVGIAHQAGFR
                     YLYTTRAFGQNRPGSDPASIHRFAVRNTGGASLGRRVRLAANPVLGPLFNRWKRWARS
                     QRRES"
     gene            25589..26335
                     /locus_tag="VFR20_08005"
     CDS             25589..26335
                     /locus_tag="VFR20_08005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0230500.1"
                     /translation="MTLTAIIITKNESAHIADCLDSVAFADEIVVVDSGSTDDTCDIA
                     RQKGARVVTTEDWPGFGPQKNRALDLATCDWILSVDADERVTPELAQSIQREMSHPRA
                     DGYLIARLSNFCGRWIRHSGWWPDRVLRLFRRESGRFTDVAVHESVRCTGTVAVLEGH
                     FLHYPYPDLETLMTKINRYSSDAAVMMHARGKTAGVASAVGHGFWTFIRIYVIRKGFL
                     DGREGFVLAAAGAAGSFFRYGKLMLLSRRK"
     gene            26376..27533
                     /locus_tag="VFR20_08010"
     CDS             26376..27533
                     /locus_tag="VFR20_08010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0230501.1"
                     /translation="MKILYTNFHQRNGGGHVTYVVNLLKALAPEHDLTVATPGSSRLY
                     RYAREVPGVEVVDLSYSSRLPRMVPEVLRLRRLLGDGRFDVVHVNASADHRHVMLACL
                     GLRQRPKIVWTKHNDHPVRSLGHWLRAHLATDSVIAVSAYVERIVLQSPYARLPLRVI
                     RHGVDTGYFSPPSAAQRRAWRHKTFGALADDVFTLGSSGGTDEDKGWLDLVAAVASLP
                     SHWRDRVRVVVAGDPPRGYRKDRVEELGMAGQVVFPGLVDDVRPILGACDAGFVLSYR
                     EALSYACRETLSLGLPTLVSDAGGLPENVEDGVDGWIVPARDVPRIAKALVWMLENPA
                     EISSMALAAREKSVNEFNLDLFAERSLYVYRQALSRAKPADGGRGSGDQIK"
     gene            27554..28273
                     /locus_tag="VFR20_08015"
     CDS             27554..28273
                     /locus_tag="VFR20_08015"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0230502.1"
                     /translation="MYHQVGIPAPRGTPYRGLTVHPSDFRRQMVWLRRLGYRGLSMRD
                     LMPHLRDGSNEKVVGITFDDGYRNVFRHAMPVLMELGFTATNYFVARQLDGSNVWDAE
                     DGIPASPLMSAVEMRGWHEAGFEVGSHTLDHVHLPALSPELADYQIRQSKLELEQLLE
                     AEVTAFCYPYGDESADVRGMARRAGYSNATTTERGLARQTDDAFGLPRVVVSRSTNII
                     RFLVKCTTRLEDRRRTNRAIA"
     gene            complement(28365..29132)
                     /locus_tag="VFR20_08020"
     CDS             complement(28365..29132)
                     /locus_tag="VFR20_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEU0230503.1"
                     /translation="MALFSSFLTSLIIPLNLCITCLVIGTVCFIARRRKTGFIIAMAG
                     IGWAVFWSLPASSLWAGGRLEQMYPHMLPAQLPKAQAIVVLGGNTANNRQNWFEPYDK
                     DTAWPRIDTAALLYRAGRAPTIILSGAALEGKVSEAQAMAHALEKQGIPELALIMETH
                     SYTTHQNGTFTAAKLRALHIKRILLVTSALHMPRAMAVFRKLGVSAIAAPAPRQIYVP
                     GQPGFAFWLPDMRSLDASRSIIKEYVGLLVYWLRGWV"
     gene            complement(29141..30100)
                     /locus_tag="VFR20_08025"
     CDS             complement(29141..30100)
                     /locus_tag="VFR20_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEU0230504.1"
                     /translation="MPIISAIYIRLPNWIGDVCMSLPSLRTVLNTDIPVTVCAKPWAR
                     DLLAGFGHAGFIEMKGRWHEDRAAVSNHRKQARHVHARGLLLPDSLSSAMVFKFAGIP
                     SAGYRDDGRSLILRWPIDKPAASTHAVRAWHNVAAQALKRWNLPVHPSEPGPELGLKP
                     TGKQREAALAILAGAGLEANRFVLIAPTATGLHHGNVKVWPHFDTLTRALQAKGYAVA
                     MCPPPTEVAEARHNAPSAVCLPALSLGAFATLTQLASLVICNDSGVSHLAAASNAKQL
                     TLFGVTQQERTGPWSDHATCLGAAHQWPSPAEVERTADALLRL"
     gene            30129..31994
                     /gene="msbA"
                     /locus_tag="VFR20_08030"
     CDS             30129..31994
                     /gene="msbA"
                     /locus_tag="VFR20_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEU0230505.1"
                     /translation="MGSSPHFYPIFDNGKIGASSDCIKASVFLKPSPASAPSHAEPVK
                     FDIWKRIYSRLGPYWKAVVLAMVLLSVAAATQPTLAVIMKPLLDGGFSGAKPSYVWSI
                     PLAVVGLILLRGLCGFFSDYLLAWVANKMLLGIRREMFERLLGMPDTDFKRGDTGRLL
                     NRFTIDANNVTSSATDVITVLVRESLTVVALIGVLLYMSWQLTLIVLVILPLSVVISR
                     IFTRKLRRVNRDTVNMNAELTRVVGDGINGQRVIKLFDGYEYESGRFEYINGRLRRFA
                     MRAAVANAAMSPLAQFTIALSVAVVIAVALYQANSGVLTVGSFASFMAALGQIFDPVK
                     RLTNISGRMQRMLVSAESVFTLIDHETEADTGKLSLDRVSGHIEFRDLVHRFEDASND
                     TLSNVSFVVEPGQTVALVGRSGSGKTTLVNMLPRFIHPSSGQVLIDGQDIDGFTLRSL
                     RSQLSLVSQDVVLFDGTIGDNVNYGALHDASQDEIRGALEAANLLAFVDSLPNGVDTL
                     VGENANLLSGGQRQRLAIARALIKNAPILVLDEATSALDNESERQVQASLERLMAGRT
                     TLVIAHRLTTVQKADRIVVLDEGRIVEHGRHDELLALDGLYASLYRMQFRDVVGA"
     gene            32056..32469
                     /locus_tag="VFR20_08035"
     CDS             32056..32469
                     /locus_tag="VFR20_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230506.1"
                     /translation="MRKPLLCLGVVVLAGCAAPHGLTRYVDLHSEDHLRAQRHILMDF
                     PKIQMALFEHQRACGSAPVFTRSSKDPSYAMITQKLSPGADWKNTIEVDLALLGDLTV
                     QAKAYTYHAGLEAQIDQVFNAIAKPKVCAERKKAG"
     gene            complement(32516..33979)
                     /gene="rng"
                     /locus_tag="VFR20_08040"
     CDS             complement(32516..33979)
                     /gene="rng"
                     /locus_tag="VFR20_08040"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEU0230507.1"
                     /translation="MTEDILINVTPFETRVALIEQGAVQELHVERSIQRGHVGNIYLG
                     KIVRVLPGMQSAFVDIGLERAAFIHVADLQQNRGERGSGGNMTPIEKLLFEGQSLMVQ
                     VIKDPLGTKGARLSTQISIAGRMLVYLPHEPHIGISQKIGSEQERAQLRERVQNLMPE
                     NEAGGYIVRTQAEIATDDELKADQTYLRAIWQSIQDKARRQAAPSALYTDLTLAQRVL
                     RDMVGPETSTILVDSRTTTHALREWALQYTPSVLERINHYSGDRPLFDTAGVDEEIAR
                     ALSRRVNLKSGGYLIIDQTEALTTVDVNTGGFVGGRNFNDTIFKTNLEATVALARQLR
                     LRNLGGIIILDFIDMDDAGHRASVLAELGKALARDRTRITVSGFSQLGLVEMTRKRTR
                     DSLAHQLCEPCPTCQSRGSVKTARTVCYEILREILRESRQFNPKEFRILAAQGIVDLF
                     LEEESTHLAMLGDFIGKAISLEVETQYSQEQYDIVLM"
BASE COUNT         6053 a        10376 c        11388 g         6814 t
ORIGIN      
        1 cgcgacgtgg cggtggagtt cttcaccatg agcaagagct acaacatggc cggatggcgt
       61 gtcgggttca tggtgggcaa tgcggaactg gtcaacgcgc tggcgcgcat caagagctac
      121 cacgattacg ggacgttcac gcccatacag gtggcggcca tcgcagcgct cgaagggccg
      181 caagactgcg tcgccaaggt ggttgaacag taccgcaacc gccgcgacgt gctcgcccgc
      241 ggcctgcgcg acgcgggttg gaatgtcgat gtccccaagg catccatgta catctgggcg
      301 cgcattcccg aaccctacca gagcctaggc tcgcttgagt tttccaagcg gcttctggcg
      361 gacgccaagg tggcggtgtc gccgggaatc ggttttggcg actacggtga cggctacgtg
      421 cgcttcgcgc tcatcgaaaa cgagcagcgc acgcgtcagg ctgtgcgggg cattcgcgat
      481 atgttccgca aggatggatt gatcaaatga gtgttgtgaa agttggcctg ttgggctttg
      541 gcgtggtggg tgccgggacc tgggaagtac ttacccgcaa cgccgaggaa atcgcccgca
      601 gggcggggcg ccgcatcgag gtggtggcgg tctcggtgcg caatgtggac aaggcacggc
      661 gcgtggtggg cgatcacgtc atggtcacca cgaatgggtt ggatgtggtc agccaccccg
      721 acatcgacat cgtggtcgag ctcgtgggcg gcgacacggt cgcccgcgaa tgggtcatgg
      781 aagccatccg acgcggcaag cacgtcgtca cggccaacaa ggcattgctc gccaagcacg
      841 gcaacgagat tttctcccaa gccagggcgc agtccgtcat ggtggcgttc gaggcggcgg
      901 tggcaggtgg catccccatc gtgaaggcca tccgggaagg cttgaccgcc aaccgcatcc
      961 agtgggtggc gggcatcatc aacggaacga ccaacttcat cctgacggaa atgcgcgcgc
     1021 gcggattgtc gttcggcgaa gccctggccg aggcgcagcg tctgggctat gccgaggccg
     1081 atcccacttt cgatatcgaa ggcgtggatg ccgcacacaa actgtccttg ttggcatcac
     1141 tggctttcgg cattccagtc cagttcgaca aggtgtatgt cgaaggcatc tcgaagctgg
     1201 cccaggaaga cattgcgcac gccgaacgcc tgggctaccg catcaagctg ctgggtatca
     1261 cgcggcggcg ccccgagggc atcgaattgc gggttcatcc cacgctcatc ccgtcgggac
     1321 gcctgctcgc caacgtcgag ggcgccatga atgccgtgct cgtgagcggg gacatggtcg
     1381 gacccactct atactacggt cagggcgccg gggcgctgcc caccgcatcg gcggtggtgg
     1441 cagatctggt cgacgtggcg cggctcgatt cggccgaccc ggatcacagg gtaccctatc
     1501 tggcgttcca gcccgatgcc ctggacgaca ccccggtgct gcccatcgcc gaggtcctgt
     1561 cctcgtacta cctgaggttg cgggtggaag accgccccgg cgtgctggcc gatctttccc
     1621 gcattctgtc cgatgcgggt atatccattg gatctatgtt ccaggagccg cacggcgaaa
     1681 ccgatgcgga catcatcttc ctgtcgcacg aagcgcgtga gggtgcggtc gatgccgcca
     1741 tcgagcgcat ccgtgacctg ccattcgtca aatccggcgt tacccgcttg cgggtcgagc
     1801 atctgctatg aaatatcgtt ccacccgtgg cggcatggcg ccgcagtcat tttccgacat
     1861 cctgctcgag gggctggctc ccgacggcgg actcgccgtg ccggtatcgc tgccgcgagt
     1921 cgagcgcgcc acactagagt cgtggcgccc gctcgcatac gccgacctgg ccaccgcggt
     1981 cctggggctg ttcatagacg atattccgac ggccgatctg tcgcgcctga cccatgccgc
     2041 ctatactcca cggcttttcg gcagcaccga aatcgtgccg ctcaaaccgc tcgatggcgg
     2101 gttgatgctg ctcgggctgt cgcaaggccc gaccctggcc ttcaaggaca tggccatgca
     2161 gtttctgggc caggttttcg agtatgttct tggcaagcgc ggcgcgacgc tcaacatcct
     2221 gggggcgaca tcgggcgaca ccggttcggc cgccgaatac gccttgcgcg gcaagcatgg
     2281 ggtgtcggtg ttcatgctgt ccccccacgg ccgcatgagt gattttcagc gtgcgcagat
     2341 gtattcgctg caggatgcga acatccacaa catcgccatc gagggtgtgt tcgacgaatg
     2401 ccaggatatc gtcaaggctt tggccggtga tctggccttc aaatcacgcc accacctggg
     2461 tgctgtcaac tccattaact gggcccgcat cgcggcgcag gtggtgtact acttctgggg
     2521 gtggctgcgc gccaccgatg cgcttgacgc cacgggaaag cgcgaagtgt cgttcgcggt
     2581 gccgtccggc aattttggca atatcctggc ggggcatttc gcgcgcgcca tgggtctgcc
     2641 ggtgcgccgt ctggtgctgg ccaccaatga aaacaacgtg ctggaagagt tctttcgcac
     2701 tggcgtctat cacccccggc cggcggccca gacctacgcc acgtccagcc catccatgga
     2761 tatttcgcgc gcctccaact tcgagcgttt cgtgttcgat ctggtgggcc acgatgcgca
     2821 acgcgtgtcc gacctgtggc ggcggttggg ggagaccggt gcattcgacc tgagcgacct
     2881 gcgcccggaa ttcgagtctc gctacggttt cgtcagcggg gtcagcacgc atgccgaccg
     2941 tctgtccacg atacgcacgg tgaacgaacg cacaaacgtg ctggtcgacc cccacacggc
     3001 cgacggcatc aaggtggcgc gtgagcacgt ggaggaaggc gtgcccatgc tggtgctcga
     3061 aaccgcgctt cccgccaagt tcgatgccac gattcgcgaa gccgtggggc atccggcgcc
     3121 ggtgcccgac caccttcagg gtctggcccg cctcccgcag cgcgtgcggg tcatgccgtg
     3181 tgacgcgggc ctggtgcgtg agtacatcgc gcgccacgcc atctgagact actcgagtgt
     3241 ggtgttgacg ctatcaggaa gcgcggtagg tcttcagatg gaagctggtt tcggtgttgc
     3301 tgatgccgcg aatcaggcgg atgcgctcca gcacctgtga caagtcggca agattggtgg
     3361 cacgcaattc cgccagaaga tcccatcgcc cgttggtgtc gtgcagcgcc gccacaccgg
     3421 gctcgcccaa caggctggca atgacgatac gggtttcgtt tccttcgatg gcgatgctca
     3481 tccatgcgcg gatttcgttg ggctgggcgt ccggccgcag cctgacggta tagccaacga
     3541 tgacgccgtc gttctccagg cgtgcaatgc ggttgctgat ggtgccgcgt gaaacgtcca
     3601 acttcttggc cagcgttgcc acagtcagcc gggcattctt tcgcaacaga gagatcaaag
     3661 cgtggtcaag cgcatccatg atgtcgttat ggcaaattgt attgccataa tgatagattt
     3721 ctgctgaaaa gcttgcaata ctgacgattc actttatctc ctcccaatgc cacactgaac
     3781 ttcatggaag cgtcgggaac atcgggcctt ggcccgcatc gatgcatcgg cgcgacggtt
     3841 tcgaagatca aggagatacg atcatgacca cccggaccaa ggcagtgaac gatgcccgcg
     3901 cggcgaccct gtatctggac gtggcggaca tcgccaccat atttcgcagc gaaggcgccg
     3961 cagcggtgtt ttcccggttg ggggactaca tccgcaatga ctacctgcgt tgggacgatt
     4021 tcgacaagtc gccccgggtc gccaagtact cccgtcttgg ggtcattgaa ttgatgccga
     4081 tcgccgacga caccacctat agtttcaaat gcgtgaacgg gcatccgcgg aatatcgagc
     4141 gcgggttgcc gacagtcatg gggtttggcg ccctggccga tgtcgacacc ggcgccgtca
     4201 ggctgatcag tgagctgacc ttgacgacgg ctttgcgcac cgccgcgacc tcggccttgt
     4261 tcgccagcgt gctggcgcgc cccgatagcc gcagcatggc actgatcggc aatggcgcgc
     4321 agtccgattt ccaggccctg gcattccatc atcttctggg catcgaggaa atcaggttgt
     4381 tcgacatcga tgacgccgcc accgacaaac tgctgcgcaa ccttcattcg accgacatca
     4441 ggttcaaggt ctgcgacagt gttgcccagg ctgtgcaggg ggccgacatc gtgacgacgg
     4501 ccacggccga ggacgcgcac gcccaggtgc ttcgcgccgg catgatcgag cccggcatgc
     4561 atctcaacgc cgtggggggc gactgccccg gcaagacgga actggatccg gaaatattgc
     4621 acatggggcc ggttttcgtg gagtacgagc cgcagacccg ggtcgaaggg gagtcgcaga
     4681 acatgcccgg ggattttgtc gtgaccccgt tctggaaggt gctgcggggg ttggagccgg
     4741 gacggaccag tccggggcag atcacgatct tcgattcggt gggcttcgcg cttgaagatt
     4801 attccgcctt gcggtatgtg cgcgacatgg catgtaaact cgggttgggc cacacgctgg
     4861 cgctggtgcc gcggctcgat gacccgaaga acgtcttcag tctgaccgag gcccggaacg
     4921 tccgcgagtt ctgcggacaa ggagtgacac agccatgcga gaaggaagca atgttgccgt
     4981 gagcctgatt ggtgccccca cggatatcgg agcgggctgc cggggtgcca gcatgggccc
     5041 cgaagcgttg cgtgtggcgg gtatcaccga ggcgctggcc cgcttgtcga taccggtgcg
     5101 cgattgcggc aacttgtccg gccccttgaa tccggacgcg ccgatgcgtg aagggtatcg
     5161 ccatttcaat gaagtggtgc ggtggaaccg cttggtgcat gacgccgtcc acgccgaact
     5221 ggccgacgga cgcctgcccc tgatgttggg gggtgatcat tgtctgggtg tggggtccgt
     5281 cagcgcggtg gcgcgttatt gccgcgagca ggggcgggtg ctcaaggtgt tgtggctgga
     5341 tgcgcatgcc gacttcaaca ccgccaccat cacgcccagc gggaacatgc acggcatgcc
     5401 cgccgcctgc ctgtgcggat atggcccctc tgaactgacg cgcctggggg gcgagcctcc
     5461 catcctgccg cctgaagcct tgcgccagat cgggctacgc agcgtggacg cacaagagaa
     5521 gcggcttatc cgcgacgttg gcatcgaggt ctacgacatg cgcgccatcg atgaacaggg
     5581 catgcggcag atcatgcgcg cggcgctacg cgacgtcgac ggcgacgtgc atctgcatgt
     5641 cagtttcgat gtcgattttc tcgacccgga gatcgcgccg ggggtcggga cgacggtgcg
     5701 gggcggcccg ggttatcgtg aggcgcagct ttgcatggag atgatcgccg atacgggccg
     5761 catgggttcc ctggacatcg tcgaactcaa tcccgcgctc gatgtgcgca acgaaaccgc
     5821 tgaactggcg gtcgatctgg tgggcagcct gttcggcaag cgcacgctcg cgtagtgggg
     5881 ggaaatcagg ttcttgaagg cgtgccggcc ttactcggag tgaaccggct cgcagatcac
     5941 gtcggtcttc accgagctcg cgatgaagca ctcttcgtgc gcttgttcgt gcatttcgtt
     6001 gatttgattg tgagtgggct gcttgtcccc cgagaaaatc acctccggcc tcaacgtgac
     6061 ccgtatcatg gcccagtccc cgtcgcggtt ctcgcccatg acgcccacgg ccttgtccac
     6121 gtatcggtcc acgcgaaacc cctgcttggc ggccatggcc agaaaccaca gcatgtggca
     6181 gcttgacaag gcagaaacga aaagttcctc ggggtcgacc gcagtggaat ccgatagcgg
     6241 cactggaacc atgtgcggcg aggccgacgc cgtgatctgc gcgccgccat cgaagcgcag
     6301 gatgtgcctg cggctgtatt tcttgtcgag gaaatcctcg tcatttcgga cccagatgat
     6361 ctcggcggca tattcagcca tggttgctcc ttgatggttg ggcatcgatc gcagaggcga
     6421 gtcgaggttg cacggttgcg ggcgatcgag ggctggttcg ggggaagggg gaagggcggc
     6481 ggccacgccc gttgcgggtg ggcgtttcga ttcaatcata aagccaaaac ccccaaagcc
     6541 aaaccgtccg catggtcgcc gccgccggcg atattcaccc gcccaggcga cgcaccgtgc
     6601 cccgtccacc gccgacccag cctttccagg cgagccagaa cttgcggatg ggggtcaatg
     6661 cgacacgctg atccaacaca tgccattgat cgcgctcgag ctcgccgagc aacgtgtggt
     6721 ggattgacgc cagcatcagg ccggggcgct gagctcggcg atccgcctcg ggcagggccc
     6781 gcaccgcgtc gcggtagcag tcgcgggcgc gttgtgtttc gaagcgcatg agcgccacga
     6841 atgcgtccgt gtgtcggcgg ttcatgatgt ccgcggcggc gaccccgaag cggtccatgt
     6901 cgtcggtggg cagatagatg cgccctcgcc gcgcgtcatc gcccacgtcg cggatgatgt
     6961 tggtgagttg taaggccagg cccagttttt ccgcgtacat caaggtagcg gggtccgtat
     7021 agccgaaaat acccgcggac atctcgccca cgacgccggc ggcgtgccag cagtactcgc
     7081 gcaggcttgg ccagtcgggg tagccaggct ggtccagatt catctcctcg ccatcgatga
     7141 ccgcatgcag gcgctctgcc gtgatgccat atggttggac gtgcgacgcg agcgcggtca
     7201 tgacgggatg ttcggtgctt ccagtgaaaa gccggtccac ctgggtgcgc caccaggcca
     7261 gcttgatgcg cgccaccgaa ctctcggtgg tttcatcgac ggcatccttg acctcccggc
     7321 aaaaggcatg cagagccgtg atcgcgcgtc ggcggtcggg cggcaggaac agaaacgcgt
     7381 aataaaagct cgagccgctg cgcgcggcct tgtcttggca atattcgtcg gggctcatgg
     7441 ttgttttcgg tatgcgggcg gtgacgcgtg cgccagtgta gcggttggcc ggcgcagggg
     7501 cgcatcagtg tcccgccatg cccgcgtcgt caaagtccgt gccgtcaaag agggcgccgg
     7561 ggaggtttcg tggcgaacag cgaccgcgta tcgaggtcgt ggggaaatcc gaccatttta
     7621 ctgaagagcg gtcgcggagt ccggcatcgc cccggtcggc gttgcccgag cgtcgcgtgg
     7681 cgaactggat tagactttgg ctctctggag aaaagcatgc ctcgtccaat tctggccacg
     7741 atttcattgc cgtccctgac acacaatctt gcggtcgttg ccggccgttt gaatgacgcc
     7801 cggcctgccg ggcgcatggc gccgttcatc tgggcggtga tcaaggccaa tgcctatgga
     7861 cacgggatcc tgaacggcat gcgggccttc tccggggccg atggcctcgc catgctcgat
     7921 ctggacgagg ccgtgtgctg ccgggaagcc ggttggtctg gcccgattct cctgctggag
     7981 ggggggttcg agccttccga tctctccgtc atagacaggc accgcctgac tgtcaccgtg
     8041 cattcccagg ctcagctcga tatgctgcgc ggagccgcgc cgcaagcgcc catcgatgcc
     8101 atgatcaaac tcgattccgg catggggcgc ctggggttcc cgcccgacgc ataccgtgat
     8161 gcctaccgac aggcgcatga cttgaagcag cggggcatac tgggcaacct tggaaaaatg
     8221 acgcatttcg cgcgcgccga tgacgacccc ggggtcacgc gcgcgcaact gcgcgtgttc
     8281 gacgaggtta ccggcgggct tcccgggcgg atcagcgtct gcaattcggc ggccacactc
     8341 actccagggc tctggtccgg gttgccggaa ccatatgaac aatgggtgcg gcccggcatc
     8401 tgcctgtatg gcgcctcgcc gttcgccgat cggccggctt ccagctttgg cctgcgaccc
     8461 gccatgacgc tttcggcgga gctcatcagc gtgcgccgca tcccggcggg ttacagtatt
     8521 ggctacgggc accggttcac cgcaagcgac gccatgacca tcggtgtcgt gtcgtgtggt
     8581 tatgccgatg gctatccccg gcatgcgccc accggcacgc ccattgtggt cgccggcgta
     8641 gccacgcggg tgctgggccg tgtgtccatg gacatgctga tggtcgacct ggctcccgtg
     8701 cccgacgccc gggtcggtct accggtggtg ctgtggggag agggtggtcc gtccgtcgat
     8761 gacgtcgccg cggcctgcgg cacgatcggc tacgaattgc tgtgcgcggt ggcaccgcgc
     8821 gttccccgca aagtggtttc cggctgatgt cacgttgccc acacttggag aagaatccgt
     8881 gaaagacaaa tgcgtgctgg tcaccggtgc gaccaagggc ataggctggg ccatcagccg
     8941 ccggctggcc gatcagggat gccatgtggt gggtctcgcg cgccacacac aggacattga
     9001 ttttcccggc tatctctatt cctgcgatct ggccaacgcc ggcgaaaccg aagagactct
     9061 acgcgtcatc cgggaaaaat acccggtcga cgcggtggtc aacaatgtcg ggatcgtgat
     9121 gccccagccc ctcggcgagg tcgagctgac cgccctgtat ggcgtgctgg acttgaacgt
     9181 gcgggtggcc gtgcaggtgg tccagacttt cgtggaatcc atgaaagccc gtcgggaagg
     9241 gcgcatcgtc aacctatgca gccgcgcgac cgggggggct ctgaaccgca ccagttattc
     9301 cgcggccaag agcgcgctca taggctgtac ccgcacatgg gcgctggagc tggctgagta
     9361 cggcatcacc gtcaacgcag tctcgcccgg ccctgtggag accgagttgt tccgcgccaa
     9421 ccaccccgtg ggcagcgagt ccgaggccca ggccctggca tccattccca tgaagcgtct
     9481 gggccaaccc gccgatgtcg cggccgccgt ggtgtttctg ctgagtgacg aagccggtta
     9541 catcaccgga caggtgcttg gggtcgatgg cgggggaagc ctgtaattcg aggctccaga
     9601 gatcctccga acatggatgg ccacgccggt cttcgttccc tggccctgcg gttaaactcg
     9661 cgcaatggct aaaacgaaat cctcatatgt ctgcaatacc tgtggcgcct catacccgaa
     9721 atgggaaggg cgctgcgcgc aatgcggtga atggaattcc cttcaggaaa ccctggcccc
     9781 gggggctgat gcgcatcggt tcgcatcgct tgccggcacg gcgcccgtgc agcgtctggc
     9841 cgatatcgag gctcgcgaga tcccgcgcca acccacgggc ctggcggaat tcgaccgggt
     9901 cctgggtgat ggcctcgtgg atggcggggt ggtactcatc ggcggcgatc cgggaatcgg
     9961 caaatcgact ttactgttgc aggccatggc ctccctgtcc cgcacgacgg gcgtgctcta
    10021 catcaccggc gaggagtccc ccgagcaagt cgcgttgcgc gcccagcggc tggaaatcgc
    10081 cggcactggc gtcgatctgc ttgccgaaat ccggctcgaa gcgatcatcg atacgttgac
    10141 cgagcgccgg cccggggtgg ccgtgatcga ctccatccag accttgtact catcggcgct
    10201 gagcgcggcg cccggctcgg tatcgcaggt gcgcgagtgc gcagcgcaac tcacccggct
    10261 cgccaaacag acgggcatca ccatcatcat gattggccac gtcaccaaag atggctcgct
    10321 ggcggggcca cgggtcttgg agcatattgt tgatacggtg ctttacttcg agggtgatac
    10381 gcattcgtca tatcgactgg tgcgcacctt caagaaccgc ttcggcgccg ccaacgaact
    10441 gggtgtgttc gccatgaccg accatggtct gcgaggcgtg agcaacccgt ccgcgctgtt
    10501 tctgtcacgg cacgatcaag acgtggcggg gtcgtgcgtc atggccacac aagagggcac
    10561 gcggcctctg ctggtcgaga tccaggcgct ggtggacggc gcgcacgtgc ccaatccgcg
    10621 caggttgtcc gtcggcctgg agagcacacg attgtccatg ctgctggcgg tgctgcatcg
    10681 ccatgccggc gtggccacct tcgaccagga cgtctttgtc aatgcggtgg gtggtgtcaa
    10741 gatcgtcgaa ccggcggcgg atctgccggt gctgctggcg atcatctcgt cgttgcgcaa
    10801 ccgtccgttg cccaaggggc tggtggcgtt cggcgaagtc ggcctggccg gcgagatacg
    10861 gccggcgcca cggggccagg agcgcctgag ggaggccgcc aagctgggct tcagcgtggc
    10921 gctcattccc aaggccaacg cgccaaggca gccgatcgaa ggtctggaga tctgggcggt
    10981 ggatcgtgtc gacgccgcgc tggcccgtgt ggcgtgagcg cggcgtcggt atttgaatat
    11041 cgccgcccaa cggcggcgct ttagaggggt ggagcgttgg agtggtatgt gattgtgccg
    11101 gcttgtctgg cggcgggggc ggcgataggc tttgtcggcg gagtcctggg catcggcgga
    11161 gggctgatcg ccattcccat gctgggtatt atcttgaaca tgacccagca acaggcgcaa
    11221 ggcacggcac tgatcatggt gctgccggcg gtgctgatgg cggtgaggaa atacaaccag
    11281 cgggcccgca tcgatctgag ggcggcggcc gcgggggcgg ctgcctccat cgtgttcacc
    11341 tgggtcgggg cgaaaatcgc gctgggggtc gacccgatcc tgctgcgccg cgtctatgca
    11401 gcgttcgtcc tgtgcatcgc cattttttac ttttacaaag ggatggggcg gcatgccaag
    11461 gcgtcgcagc cctcgtctgt tcgcagcgcc tccgaattcc acaagggctg gttcgccttg
    11521 gtcggcgtac tcgccgggct gatcggaggg gtgttcggcg tcggaggctc ggtcatggcc
    11581 gtgcccatcc tgaccaccat cttccgcttg ccgcagacgg gcgcccaggc gctcgccttg
    11641 accatgatca ttccaggctc caccgtcgcc ttgatcacct atgccacgca tggccagtcg
    11701 gattggctgg tggggttgcc actcgcgctc ggaagcattt tcattgtgcc gcatggcgtg
    11761 cgattcgcct atgcgttgcc ggaaccccgg ctgaaagtcg tgttcgcctg catgctgctg
    11821 gtcatcaccg ccttgctgct ggccaaggcc tagtatcgtg agagcatact aatggtttcg
    11881 gcgcctccca ccaccatggc cctggcctcg ggcgccatgt gtttcgatcc ggcattggtg
    11941 cgtgcgcccg gggcgttttt tttcgatccg accgaatccc gcctgagcgc ggaaccggtg
    12001 agcactggtg gccgccaggc cgcctggttc gtgacagggg agttcggtga aggcgttctg
    12061 cggcactacc agcgtggggg catgatcgcg cgccttagcc gatcccggta cctgtgggcg
    12121 ggtgcgagcc agacccgctc gttcgccgag tttcgtctga tgcatgccat gcgtgagcat
    12181 gggctggcgg ttccccggcc catcgccgcg gcatactggc gccatgggcc gacctacacg
    12241 gcggcgatca ttgtcgaacg catcgcgggc gctgtgccac tggcctgcgc gatggacccc
    12301 gtctcgtgcc ccaaggtggc cagggcgata ttcgacatgc atgaggcggg gttctggcac
    12361 gctgatctca acgccttcaa catccttttc gatggaggcg gccaggtgtg gctgatcgat
    12421 ttcgaccggg ggcgccgttg ccgtctcacc tcgcggcgcc gcgcggccaa tcttgccaga
    12481 ctcaggcgtt cgctgatcaa ggtggcgggc gcggggggcg agcgctactg ggtcgggctc
    12541 gatgtcgcgt atcgacggct tgtcatgggc aaacccggtg acgaggcatg aatagcgcga
    12601 tgcggcgcga acccatcggg cgggcgtcgg tgattcacat gatatgcagg aaattacagg
    12661 attttctcca tgatgattcg ggttttcggt ttgaacaagt gcgacacctg tcgaaaggcg
    12721 agagcgtggt tggacgagca cggcgtcgcc tatgatttca tcgattatcg cgacaatcct
    12781 gttcccgcct cgacgctggc gcaatgggcc tcggcggtgg gtggttggga caaactggtc
    12841 aaccgcgcat cgctgacatg gcgcggcctg ccgcccgagc gcaagtcgcc cgagtctccc
    12901 gcgcaatggc tggcattgat cgccgagcat ccgacgctgg tccgtcgacc ggtgctcacg
    12961 cggcttggcg aggatcccat caccggattc tccgacaaaa agtatgctga aatattcaaa
    13021 taacacgagc ctgccccacc gtactccaag gaagatcacc caaccatgct gacccagcaa
    13081 gaactcaaac agaacgtggc gcgtgccgcg ctcgattatg tcattcccag ccttgatccc
    13141 aatgtggtgc tcggcatcgg taccggatcc acggtggatc tgttcatcga tgcccttgcc
    13201 gcgcacaaga accggttccg ggcggtggtt tccagctcgg agcgcagcgc ggcgcgcttg
    13261 cgcggccacg ggatcactgt gctggatctg aacgaggtgg cctccatgcc cttgtacgtg
    13321 gatggcgccg acgaaatcga tgatgcattg cacatgacga aaggaggtgg aggggcgctg
    13381 acccgcgaga aaatcgtggc gtcggtggct gagcgttttc tctgcattgt cgacgaaaca
    13441 aaatgcgtgc ggcggctggg ccgtttcccg ttgccggttg aggtcgttcc catggcgctg
    13501 cgagtcgttg cgcggcgctt ggagaaactg ggtggcgcac cgcgtgagcg cgatggtttc
    13561 gtgaccgaca atggctgccg cattctggat gtgtcggggc tcgacatagc cgacccggcg
    13621 aatctggaag ccaccatcaa cgacatcccg ggcgtggtca cttgtgggct gttcgccatc
    13681 gccggcgccg atctcgcgct ggtgtccaca caggatggcg tgcgcgaact gacaccgggt
    13741 cggtcacaat aacgtcatat ccggcatgta gacttatgaa ttgaccttgt tcgctacgca
    13801 atccaagggc cgatatgctt gagcacacca acaaacagtt tcacaccgat ctcgaagaga
    13861 cacgttccag gttcctgcag atgggcgggc tggtcgagtc catggtgcgg gatgccatgg
    13921 tcgtgctggc cactggtgat caggccatcg tcgatcgtgt gcgggagcat gaaaaagccg
    13981 tcaacaagca cgaggtcgag atcgacgagc gcatcgggct gctcatcgcg cgtcatcaac
    14041 ccacggctgg cgatctgcgt ctgcttttgt ccatctccaa gatgctgacc gacatggagc
    14101 gctgtggcga cgaggccgag aaaatcgcca aggtcgcgcg gcgtctatac gaggccgaca
    14161 gccgctatac cccggccgtc gatctggcgc acatcgccga ctgcgtgaca acgatgatcc
    14221 gtgacgcgct ggattccttc gccagggaag atccgctgca tgcggccgag gtcgtgcgtc
    14281 gtgacaaatc ggtggacaag gaatggaagg cgtcgctgag gcagatgatc acctacatga
    14341 tcgaagaccc gcgcacgatc tcgcgctcca tcgatctggt tttcatcgcc cgttccctcg
    14401 agcgcatcgg tgatcatgcc aagaacatgt ctgagcgcgt gatctacatg gtgcgcggcg
    14461 acgacgtgcg gcacaccggc gtcaagaatg ccgagcgcac ggcgcgcggt gaagccggtt
    14521 ctgacgacgg cgtctgaatc ggtttgctca gcgtgacggg ggcacgaagc cctgggcttc
    14581 gacgtgcccg tcttcgaaca ggaagcgctc catctggccg gccaggaatt tccgggcatc
    14641 ggggtcggcc agattcagat ggttttcatt gaccagacgg gtttgggtcg agatccactg
    14701 ggtccaggct tccttggaga tgtgctgcca tatgcgcttg ccgagttcgc cgggataggg
    14761 cggaaagtcg agcccctcgg cctcgcgttt gagcttggcg cattggatca tgcgtgccat
    14821 gtgtcgattc ctgatgatga atgccgtgtg tagtgtacaa ccggtggcac tggctggtgg
    14881 agcgcccccg gcgggtgatc acagccgttt gatgaatatc aggctgttgc gggaatggtt
    14941 gtaatgcgcc cgcttttcgt ggggcaggtc ggaaacgccg ccctgtacga agccccgctt
    15001 caggaaccaa tgcgaagtgc gcgtcgtcag tacgaacagg cgtttcatgc cctgggcgcg
    15061 cgcctgcgat tcggtgtggc gcaacagaat ttcgccttcg ccggttccct gccattcggg
    15121 atgcacgatc aggcaggcca tttcggccat gccgtcattc gggtatggca tgagcgacac
    15181 gcagccgtag atcacgccgt catgctccag aaccgtgaag cgctcgacgt cgcgctcgat
    15241 gacggcgcgc ccgcgcggca ccagcgtgcc atcttcttcc agcggttcga tgagctgaac
    15301 gatggcaccg acgtcgtcga ccgtggccgg gcgcaggtcg tccagggtgt cttccaccac
    15361 catggtgccg accccgtcgt gggtgaagat ctccagcagc acgctgccgt cgagggtgta
    15421 cggaacgaga tgtgcccgcg ccacgccacg tttgaccgcg agcgaggcat gggaaaggaa
    15481 cgaggcggtg tcttcgtcca gggtgttcga cgccagaagg tcatcggcat ccttgcgcgc
    15541 cagttcggtg tcggtggtgc catcgctgtt gagcaccgtg cggtcctgcg tcaggaatat
    15601 gagtttttcc gcccgcagcg ccacggcggc gctggtggcg agatcttcca tggccaggtt
    15661 gaaggcttcg ccggtgggtg agaaccccag aggagagagc agcacgatgg cgccgtgggc
    15721 gatgatgttc ttgatggctt cgaggtccac cttgcggacg gcaccggcgt gtttgtagtc
    15781 cacgccgtcg atcacgccga ccgggcgcgc ggtgacgaaa ttgcccgaga tcacccggat
    15841 ctgggcgtgc gacatggggg tgttgggcag gccttggctg aacgcggcct cgatgtcgag
    15901 gcggatttcc ccggcggctt ccttggcgca ttcgagtgcc tcggcgctgg tgggttcggt
    15961 gccgcggccg aattgcgtgt cgtacccctt gaggcgaagt tgctcgttga cttgcgggcg
    16021 cgacccgtgc acgaggatca accgcacacc caccgccgag agcagtgaca ggtcctgcac
    16081 cagggcgttg agcgcgccgt cgcccacaag ctcgccgccg aaggcgatga cgaacgtctt
    16141 gccccggaag tcgtggacgt acggcgcgac gtcgcgaaac catcgcacga actgtgcggg
    16201 ggcgtattcg ggggcatcct gcgcggaaaa ggtgtcgtcg gtcgtggcca tgtggtggaa
    16261 agtgctggcg gaggcggaag ttggtccagg aagaacaaaa ttataattac ccttgaccat
    16321 ccccatgtga atttatgcaa gagcccgccg gactgtcgga taacgtacac actcgccccg
    16381 gtgccgcgca accagtggtt cggcggccgc ccctccatat cgactacccg gaggacctgc
    16441 cggtcagcgc ccggcgcgac gagatcgcgc gggcgatccg ggaaaaccag gtcgtcatcg
    16501 tgtgtggtga aaccgggtcg ggcaagacca cccagattcc gaaaatctgc ctcgaactcg
    16561 gccgtggcgc cgagcgcatg attggccaca cgcagcctcg tcgcctggcc gccacttcgg
    16621 tggcacggcg gctcgccgag gaattgaaga cgggcctggg cgactgggtc ggctaccaga
    16681 tccgcttcaa tgaccgtagt ggcccgggca cggccgtcaa gctcatgacc gacggcattc
    16741 ttctagccga gacgcagcgg gatcctctgc tgcgccgcta cgacacgctg atcatcgacg
    16801 aggcgcacga acgcagcctg aacattgatt ttctgctggg cttcctgttg cgcctgttgc
    16861 cccggcggcc ggatctgaag ctcatcatca cctctgccac catcgacgcc gagcgttttg
    16921 cgcagcacta ctccataaac ggacgaccgg cgcccgtcat cgaagtgtcc ggccggttgt
    16981 atccggtgga aatgcgctat cgcgccgttc aggacgatgg ccggaagggg gcgggcaacg
    17041 acgcagcctc cggcgacgag gaacgcgacc tgattgacgc cgtcgtggat ggggtcgcgg
    17101 aatgcgcggc gctgggggcc ggtgacgtgc tggtctttct gccgggcgag cgcgagatcc
    17161 gcgaggccgc cgaggcgttg cgtaaaagcc acccgatcgg cgccgagata ttgccgcttt
    17221 acgcccgtct gtcgcaggcc gagcaggagc gcatcttcca cccgagcggc aacgcgcggc
    17281 gcatcgtgct ggccaccaat gtcgccgaaa cgtcgctcac cgtccccggc attcggtatg
    17341 tggtggatac cgggctcgcc cgcgtcaagc gctattcgtg gcgcaacaag gtcgaacaac
    17401 tgcgcatcga gtccatcagc caggcctcgg ccaatcagcg ggcgggtcgc tgcgggcgta
    17461 tcggaccagg gatgtgcctc cgtctctatg acgaggccga tttcgcgggg cgtgcgcgct
    17521 tcaccgatcc cgaagtgctg cgatcttcgc tggcgtccgt cattctgcgc atgaaggcgt
    17581 tgggcctgga cgatatcgag tcgtttcctt tcgtcgaccc gccttccggt cgggccatcg
    17641 ccgatggtta ccatttgttg caggagttgg gcgcgctgga cgacgcgaac cggttgaccg
    17701 acacagggcg ggtgctgtcc cgcttgcccg tcgatccgcg cctgggtcgc atgattctcg
    17761 ccggccgtga gtggcagtgt ctcgatgagg tgctgatcat cgcctcggcg ttatcggtgc
    17821 aggatccacg tgatcgcccc atgactcaga gagaggcggc cgagacggcc cacgcgaagt
    17881 tcagggacga aaaatcggag ttcctgtctt tcgtcaaatt gtggcgctgg tacggtgagc
    17941 aagtggcgca caaggcgtcg cagcgtaaat tggtggccct gttgcggcaa aacttcttgt
    18001 caccgctgcg tttacgcgaa tggcacgatg tgcatggcca attgtccagt ctggttggcg
    18061 aacaaggctg gcgtatcaat gcctcgcccg ccacgtatga gcaagcgcac atggcgttgc
    18121 tcgcggggct gttgggcaac atcggtctca agagtgagga gggtgcgggc taccttgggg
    18181 cgcggggcat ccggttcctc atccatcccg gttccccctt gcagaagaag gcggggcgct
    18241 ggctgctggc cggtgaactg gttgaaacca gccgcctcta tgcgcgatgc gtggctcgca
    18301 tcgatcctgt gtgggtggaa cgcgccgccg ggaatttgct gcagcgttca tggtccgacc
    18361 cccgctggga caggaaggcg ggccaggtcg tcgccaatga gcgggccacg ctatacgggt
    18421 tgccgatcta cgccggccgc cgggtgcact acggccgggt cgatcctgtc cgggcccgtg
    18481 aggtgttcat ccgcgacgcg cttgtcaccg gcgatatcga gacctcgcta ccttttgtcg
    18541 tgcagaatcg caagctcatc gcggccatcg agaaactcga gcatcgggcg cgccgaccgg
    18601 acatcctggt tgacgactcg ctcattcagg ccttctatga caggcagatt cccgcggaca
    18661 tcttccagac ggcctcgctc gaaaaatggg cgaggcgact gggcgacgac caggcccggg
    18721 cgcttctgct gacgcgggac gatctcatgc gccacgaggc ggcgggtctg accaccgacg
    18781 tgtttcccaa gatgatgcga tggcatggcg tggagatggc gctggactat cacttcgagc
    18841 cgggatccct gcgcgatggc gtgacgctca ccgttcccct gttcgcactc aacaccgtcg
    18901 atgagccgcg cagtgaatgg ctggtgccgg gcatgctcaa ggagaaggtt ctcctgcttc
    18961 tgaagtcgtt gccgcagaag ctgcggcggc attgtgtgcc gttgcccgat tacgccgccg
    19021 cgttccatga acgctggttc gaacgcgcgg ccgaccccgg gttgggtttg ttcgatgcgc
    19081 ttgcccagga catctgggag cattgcaagg tgcgcgtggc gcgcaccgat ttcaagatgg
    19141 aagccctgcc accgcacctg ctcatgaatt tccgggtcgt ggacgagcac ggtcggatgc
    19201 tgtcggcggg gcgcaatcta gaccagctca aggccgagca tgcgcgggag gcccaggcgt
    19261 cgtttcagaa agtggcggcc accgatgccg acgtggtgcg tgtgctggat cacgagcatc
    19321 tgactggctg gacgtttggc gcactgccgg agatcatgga gatccggcgt ggcgggcagt
    19381 cgctggtggg ctatcccgcc ctggtcgacc ggggcgcgga ttgcgatctc gatgttttcg
    19441 acgatccctc cgaagcgcgc cgcatgcatc gtgcgggcct gctgcgcctg ttccggctgg
    19501 ccctgcgcga acaactaagg tttcaagaga agaacctggg tgatctgacg cgcctcggca
    19561 tgttgtacat gggcctggga agccaggacg agctgaggga tcagatcatc gaatgcagcc
    19621 tggcccaggc gtgcctggcc gacccctggc ccgtcgacgc ggagacgttc aaccaacggt
    19681 gcgcggaggg caagacccgg ttcggtctgg tggcccagga agtggcgcgc ctggcgggtg
    19741 acgtcctgac cgagtggtcg gccttgcaaa agaagttgcc ccaggctcgg ccctggaagg
    19801 cgagtcacgc cgatttgctg gctcaaactg atgccttgat ggcccggatg ttcatccggg
    19861 atacgcctta tgaccagttg aaacaattcc cacgctatat gaaggcggct cgtctgcgcg
    19921 tggacaaact caaggccgat gcgcaacgtg acagccgtct gatggcggac atgtcggggc
    19981 tcgtggccca ataccagcgc gcgcgttccg ccctgaaagg ggcccccgat gcgcgcctgg
    20041 acgagttccg ctggctgctg gaggaattgc gggtcgcgtt gttcgctcag gaattgcgca
    20101 cacccatgcc ggtgtcggtc aaacgcctgc agaaagcgtg ggccggcctg ggtacaatcg
    20161 cagcatgaca gatacgagcc ccgcctctcc tttcgtgcat ctgcgcgtcc actcggaatt
    20221 ctccgtggtc gacggtatcg tgcgcattcc tgaattggtt tcccggacgg tggagttcgg
    20281 ccagccggcc gtcgcgctga cggatctctc caacgtcttc gggctggtca agctttacaa
    20341 ggcggctcgc aaagcgggtg tgaaacccat cgcgggctgt gacgtctatg tgcagaacga
    20401 tgccgaccgc gacaagccgt tccgcttgtt gttgctggcc atcgatcgca atgggtacct
    20461 cgaattgtgc cggttgctca cccaggcctg gctggacaac cagtatcgtg gtcgggccga
    20521 agtgcgccgc gaatggttca aggaagtcga cggtttgatt gccctgtcgg gcgcgcgcgc
    20581 gggcgatgtc ggccatgcgc tcgaggccgg tcgggcagcg gaggcccgct cgctggcgca
    20641 ggcatgggcc aggttgttcc cgaaccgcta ttatgttgaa ttgcagcgcg ccggaatgga
    20701 tggcgacgag acctatgtgc aggcggctgt gcggctggcg gccagcctgg atctgccggt
    20761 ggtcgcgacg cacccggtcc agttcctcga tcggaccgat ttccgtgcgc acgaggcgcg
    20821 tgtctgcatc gccgaaggcg agcaactggc caatcccaag cgtacccggc ggttcacgac
    20881 tgaacaatat ttcaccagtt cgtccgacat ggcggaacgg ttcgccgaca ttccctcggc
    20941 gctggccaat actgtggaaa tcgcgcgccg ctgctccctg acgctcgatc tcggtacccc
    21001 gcgcctgcca aacttcccga cacccgaggg catctccctc gatgattata tggtggggtt
    21061 ggccgaggag gggcttgaaa agcgcatggt gcagttgttc cccgatgaag ccgtgcgtcg
    21121 tgaaaaatac ccggcttatc gcgagcgtct cgagctggaa tgcaagacca tcaccggcat
    21181 gggtttcccg ggctacttcc tcatcgtcgc cgacttcatc aattggggaa agaataacgg
    21241 agttcccgtc ggtccgggcc gtgggtcggg cgcggggtcg ctggtcgcgt tctcattggg
    21301 catcacggac ctcgatccca ttcgctacga tctgctgttc gagcggtttc tgaatcccga
    21361 gcgggtctcc atgcccgact tcgacgtcga tttctgccag gacaatcggg aacgagtgat
    21421 tgactacgtc aagcataagt acgggcgcga cgcggtcagc cagatcgcca cattcggcac
    21481 gctgggcgcc aaggccgtcg tgcgcgacgt cgggcgtgtg ctggaaatgc cgtattccgt
    21541 gtgcgacggc ctgtccaagc tcatcccctt cagccccacc gatccctgga ccctggatcg
    21601 cgcgctgtcc gacgagccgg ccttcaagga gcgctacgac caggacgaag aagtgcacgc
    21661 cctggtcgat ctggcgcggc ccctggaagg cctgacgcgc aacatcggca tgcacgcggg
    21721 cggggtgttg atcgcccctg ggaaactcac ggatttctgc cccctgtact gccagggcat
    21781 ggacggcagc gcagtgtcgc aattcgacaa ggacgacgtc gaggcggtgg ggttggtcaa
    21841 gttcgacttc ctggggttgc gcaacctgac cattctggac tgggcggtgc gctatgtgcg
    21901 cgccttcaat cccgacatgc gcgatttcga tctcatgtcg ctgccgctgg atgacgcggg
    21961 cacctacaag ctgttgagcg acggcaacac caccgcggtg ttccagcttg agtcgcgtgg
    22021 catgaaagaa ctgctgggac gtctgcggcc gaacactttc gaagatatca tcgccatgct
    22081 ggcgctgtat cgccctgggc cgctggagtc cggcatggtg atcgatttcg tcgatcgcaa
    22141 acatggtcgc gccgccgtcg actatttcca tcccgacctt gaatccgtcc tgaagagcac
    22201 gtatggtgtg atcgtgtacc aggaacaggt gatgctgatt tcccagatta tcggcggcta
    22261 cagtctgggt ggtgccgatc ttctgcgtcg tgccatgggc aagaaaaagg ccgaggaaat
    22321 ggccaagcac cgcgccattt ttgaagaagg cgcggtcaag aaagggtatg acgccgatct
    22381 ggcggtgaag ctgttcgacc tgatggagaa attcgcgggc tacggtttca acaagagcca
    22441 ctcggccgcg tatgccctga tcgcctatca aacggcctgg ctcaaggcct accatccggc
    22501 cgaattcctc gcggcgacga tctcgtccga tctcgacgac accgacaagg tccaggtgtt
    22561 ctggaaagac gcgctggcca acggtgtggc cgttttgccg cccgacgtga acgcgtccaa
    22621 ctaccggttc gagccggtcg ccgacgcgca cactgaacag ggtctgccac cgcgcaccat
    22681 gcgctatggt ctgggggctg tcaaaggcac gggccagggc gcggtcgagg aaatcatccg
    22741 cgcacgtggc gagggtgggc cgttcaccgg cctgttcgat ttctgccgcc gtgtcgaccg
    22801 ccacacagtg aaccgtcgca ccatcgaggc cttgatccgg gccggtgcct tcgacaacat
    22861 cgaagacaat cgcgcggcgc tgctcgaagc cgtcggcagc gccatcgaag cggccgaaca
    22921 agccgagcgt agtgcgaacc aggtgtcgtt gttcgctgat gacgcgggcg acatcgtcgc
    22981 ggacgagctg gcgagcgtca cgccttggga cctgcagacg cgtctgacgc aggagaagtc
    23041 cgcgctcgga tttttcttca gcggccatct tttcgatgcc tggcgcgatg aggtgcgtcg
    23101 cttcgcaccg aagccgctat ctcgtctgac gcccgggcgc ggcctgcagt ggttcgctgg
    23161 cgtgctggcc gccgtgcggg tcaagatgac gcggcgcggc aagatgctgt atgccaccct
    23221 ggacgatggc tcggcccagg tcgaggtggc gatcttcaac gaactctacg aacagcatcg
    23281 cgcacgtctc agggaagacc ggctcatcat tgtccaggga aaagtcagca ccgacgattt
    23341 cagcggtggg ttgcgcgtct ccgccgaggc ggtcttcgac ttgcagcttg cgcgtgaagc
    23401 gcgtgcccgg tcgctgcgca ttccactgaa tggcaacgcc gacgccgcgc gcctgcgcaa
    23461 gctgctcaat ccgtatcggg ccgagcccga gaacggcgtg ccaggcgtgc cggtcgagat
    23521 tgtcgtgcat cgcggcggat tccgctgtgc ggtgcggctg agcgacgatt ggcgtgtgcg
    23581 catggccgat gccatgcttg atcaactgaa cgggtggacc gcgcccgatg aggtggagat
    23641 cacctattca tgaggccgct gcgggttctc cactccgagg ccgcgaccag tttcggcggc
    23701 caggagcatc gcatctacaa ggaaatgctg gctatgcgtg agcgcggcca ccaccttgaa
    23761 ctggtctgcc aaccggatgc cctactggtc gagcgcgtgc gcgccgaggg gttcactgtg
    23821 cacaccaccc ctatgaacgg ccatgtgaac ttcatcaaga gcgtggcgaa gatccggcgc
    23881 gtattgagga aaagccgatt cgatgtgctc aacacccaca gtcggcgcga cactttgatc
    23941 gccggggtgg ccgggcgcct cgcgggcaca ccgctcatcg tcaggacccg ccatctcgcc
    24001 atcaaggcgg ggtcgttgtt gtcctacacc tggattccgc accgggtgac cactgtcagc
    24061 gactatgtcc ggatcaatct catcaaggcc ggcgtaccgc cagggcgcat cgcgacggtg
    24121 tactcgccga tcgagcttcc gcccccggtc gagcactcga ccctgcgtga tgaattgggc
    24181 ctggcggtcg atgacgtggt ggtggggtgc gttgcggtga tgcgcgaaaa gaaggggcac
    24241 atggcgctgc tggaggctat cgaaccgctg ttcaatgcca gaccggcctt gcatctggtg
    24301 ctggtcgggt caggctcgcc tgtgttcgaa cgcgtccagg cgtatgtgaa ggaacatggg
    24361 ctggcgcgac gggtccatct cctgggaacc cggcgcgatg ttccgaatct gttggcgggg
    24421 tgtgatttgt tcgcgcttgc cacagaacag gaggcctccg gcacggtgtt cgtcgaagcg
    24481 gccgcggcgg ggttgcctgt gatcggcacg gatgtgggtg gcgtctccga gatgctgcgc
    24541 gagggcgaaa cggggtggct ggtgccggtg ggcgacacgc aggctctgcg ggccgcgttg
    24601 gagcggcttg tcgatgatcg ggcactgcgc aagcgcatgg gtgaagccgg gcgcaggctg
    24661 ttccgcgacg aggggaagtt cagccttgaa acgctcgccc agaacacaga gcgttgctat
    24721 gtcgaatggt tgcgtgagag gcgggcatga gcgcccgcaa ccgcaccgtg cccgtcctga
    24781 tgtaccacca tatctcaccg gtggacggca tgatcacgac gtcgcccgtg aatttcgaac
    24841 tgcagctcgc ctggctggcg cgtcacggct accggtcgat gtccagcgcg gagttcgcgg
    24901 cgcacctggg cggccaggcc gcgccgccgc ggtcgatact gatcacattc gatgatggct
    24961 acctggacaa ctgggtctat gcctatccgt tactgaggaa atatggcttt tccgccatga
    25021 ttttcctgat cacctcattg atcggggacg ggccggcccg tccccatctg ggccagggcg
    25081 gactgcccga gacaccatcg catcgtgatt gcgggcagcg catccaggca ggcgacgcgg
    25141 attccgtcat gctgcgttgg aatgaagtcc ttgccatgcg tgattccggg gtggtcgaat
    25201 tccacagcca cacccatact catacgcgct gggacaaaag cccccgggcc gccgagaaga
    25261 accagctcat gacgcgggag ctgatggatt cgcgcgccac ccttgaagcg aggttgggca
    25321 gtgtttctga tcatttctgc tggccacaag gctattttga tgccgactac gtcggcatcg
    25381 cccatcaggc agggttccgc tatctgtaca ccacacgggc gttcggccag aaccgtcctg
    25441 gcagtgatcc cgcatccatc caccgctttg ccgtgcgcaa cacggggggg gcgtcattgg
    25501 gacgccgtgt gcgccttgcc gcgaacccgg tgctgggtcc gttgttcaat cgttggaagc
    25561 gatgggctcg gtcgcaaaga cgtgaatcat gactctgaca gccatcatca tcaccaagaa
    25621 tgaatcggcg catatcgccg actgcctgga ttccgtggcg ttcgccgatg aaatcgtggt
    25681 cgtcgactcc ggcagcacgg acgacacctg tgacatcgcg cggcaaaagg gcgcccgcgt
    25741 cgtgaccacc gaggattggc caggttttgg accacagaag aaccgagccc tcgaccttgc
    25801 cacgtgcgac tggattctgt ccgtcgatgc cgacgaacgg gtgacaccgg aattggcgca
    25861 gtcgattcag cgtgaaatgt cccacccccg cgccgacggc tacctgatcg cgcgcctgtc
    25921 gaacttctgc ggtcgttgga tacggcacag tggttggtgg cccgatcgtg tcctgcgcct
    25981 gtttcgccgc gaatcggggc gtttcaccga cgtcgcggta catgaaagtg tgcgttgcac
    26041 aggcactgtg gcggttctgg aagggcactt cctgcattac ccgtatccgg atctggaaac
    26101 actgatgacc aagatcaatc ggtattcgtc ggacgcggcg gtcatgatgc acgcgcgtgg
    26161 caagaccgcg ggggttgcct cggccgtggg ccatggattc tggacgttca tacgcatcta
    26221 cgtcatccgg aaagggttcc tggacggccg ggagggattc gtgctggcgg cggcgggcgc
    26281 tgccggaagc ttctttcgct acggcaaact gatgctgctg agccgacgga aataagcggg
    26341 acgtctgtgc ataacgccac cgttggattc ctcgcatgaa gattctttat acgaactttc
    26401 accaacgcaa tggtggcggc catgtcacct acgtcgtcaa cctgctcaag gccctggccc
    26461 ccgagcatga cctgacggtc gccacccccg gatcgagtcg cttgtaccgt tacgcgcgcg
    26521 aggtgccggg agtcgaggtc gtcgatctgt cgtattcgtc ccggctaccg agaatggttc
    26581 ccgaggtgct gcgtttgcgc cgcttgcttg gcgatggtcg cttcgacgtg gtgcatgtga
    26641 acgcgtcggc cgaccaccgc catgtcatgc tggcttgcct ggggctgcgg caacgtccaa
    26701 aaattgtctg gaccaagcac aatgatcatc ccgttcgcag cctggggcac tggttgcggg
    26761 ctcacctggc gaccgacagc gtgatcgcag tcagcgcgta tgtcgagcgc atagtacttc
    26821 agtcgcctta tgcgcggctg ccgctgcgcg tgattcggca tggggtggac acggggtatt
    26881 tctcaccgcc gtcggctgcg cagagacggg cttggcgaca caagacgttc ggcgccttgg
    26941 ccgacgacgt tttcaccttg ggtagttcag gcgggacgga cgaggacaag ggttggctgg
    27001 atctggtggc cgcggtggcc agcctgccgt cgcattggcg agaccgggtg cgggtggtcg
    27061 ttgccggcga tccaccacga ggatacagaa aagatcgcgt cgaggaactc gggatggccg
    27121 gccaagtcgt gtttccggga ctggtggacg atgtccgccc cattctgggg gcttgcgacg
    27181 cggggttcgt cctgtcgtat cgggaggcgt tgtcctacgc gtgccgtgag acgttgtcgc
    27241 tggggctgcc cacattggtg tcggatgccg gtgggctgcc cgaaaacgtc gaggacggcg
    27301 tcgatggctg gatcgtgccc gccagggatg tgccccggat tgccaaggcg ctggtgtgga
    27361 tgctcgagaa tccggcggaa atctcctcga tggctctggc cgcgcgcgag aagagtgtga
    27421 atgagttcaa cctggatttg ttcgcggagc gttcgctcta tgtctatcgt caggcgctgt
    27481 ctcgggccaa gccggccgat ggtggcaggg gttccggcga tcaaataaaa tgacttccat
    27541 gattcctatc ctcatgtatc accaagtcgg cattccggcg ccacggggca caccctacag
    27601 ggggttgacc gttcacccat cggatttccg ccgtcagatg gtctggttgc gtcgcctggg
    27661 atatcgcggt ctctccatgc gggatctgat gccgcatctg cgagatggct ccaatgagaa
    27721 agtcgtgggc atcaccttcg acgatggcta tcgcaatgtg tttcgtcatg ccatgccggt
    27781 gctgatggaa ctgggtttca cagccaccaa ttacttcgtt gcgcgacaac tcgatggttc
    27841 gaacgtctgg gatgccgagg atggcattcc ggcctcgccg ttgatgtcgg cggtggaaat
    27901 gcggggttgg cacgaagcgg ggttcgaggt cggctcacat acgctcgacc atgtccatct
    27961 gccggccttg tcgccagagc tcgccgacta ccagatccgg cagtccaaac tcgagcttga
    28021 acaactgctg gaagcggagg tgaccgcttt ctgttacccc tatggtgatg aatcggcgga
    28081 tgtgcgcggg atggcgcgcc gtgcgggcta ttcgaatgcc accaccaccg agcgcggcct
    28141 ggccagacaa accgatgacg cgttcggcct gccccgggtc gtggtgtcgc gttcgacgaa
    28201 catcattcgg tttctggtga aatgcacgac gcgcctcgaa gaccggcggc ggacaaatcg
    28261 ggccatcgcc tagtgtgccg ttcgtatcaa gaggcgccgc gcactggcga gtggaaggga
    28321 ggcaggccag gcacgacaca gttccgccgc ggcggatccc gggttcatac ccagccgcgc
    28381 agccagtaca ccagcaaccc gacgtattcc ttgatgatgg atcggctggc atccagggag
    28441 cgcatgtcgg gcagccagaa tgcgaacccg ggttggccgg gaacatatat ctgccgcggg
    28501 gcgggagccg cgatggccga aacccccagt ttgcggaaca ccgccatcgc ccggggcatg
    28561 tgcagcgccg aggtcaccag gagaatccgc ttgatgtgca gggctcgtag cttggccgcc
    28621 gtgaacgtcc cattctgatg agtggtgtaa ctatgcgttt ccatgatcag cgccaattca
    28681 ggaatgccct gtttttccag ggcgtgcgcc atggcctgtg cttcgctgac ttttccttcc
    28741 agcgcggccc ccgacaagat gatggttggc gctcgacccg cccgatacag tagcgcggcg
    28801 gtatcgatgc gtggccaggc cgtgtccttg tcgtagggtt cgaaccagtt ctggcgattg
    28861 ttggccgtat tgccgcccag aacgacgatg gcctgggcct tgggcagttg cgctggcagc
    28921 atgtgcgggt acatttgctc cagcctgccg cccgcccaca gagaggaagc gggcaaagac
    28981 cagaacaccg cccacccgat gcccgccatg gcgatgatga agccggtttt tctgcgacga
    29041 gcgatgaagc acaccgttcc aatcaccagg cacgtgatgc acagattcag cgggatgatc
    29101 agggacgtca gaaagctgga aaaaagggcc atgcttgggt tcagaggcgt aatagggcgt
    29161 cggcggtgcg ctcgacctcg gccggcgagg gccactggtg ggcggcgccc aggcaggtgg
    29221 cgtggtccga ccatggtccg gtgcgctctt gttgcgtaac cccgaacaag gtcagttgct
    29281 tcgcgttgct ggctgcggcc agatgcgata ccccggagtc gttgcagatc accagcgacg
    29341 ccaactgcgt gagtgtagca aacgcgccca gactcagcgc cggcaggcat accgccgagg
    29401 gtgcgttgtg gcgggcttcc gccacctcgg tgggtggcgg gcacatggcc accgcatacc
    29461 ccttggcttg cagtgcccgc gtcaacgtgt cgaaatgagg ccagactttg acgttgccat
    29521 ggtgaagtcc cgttgcggtg ggggctatca ggacaaaccg attcgcctcc agaccggccc
    29581 cggccaggat cgccagggcg gcttcacgtt gcttgccagt tggcttcaag cccagctcgg
    29641 gtccgggttc cgaaggatga acaggcagat tccagcgctt caaggcctgt gccgccacgt
    29701 tgtgccaagc acgcactgcg tgcgtcgagg ctgcgggttt gtctatgggc cagcgcagaa
    29761 tcaggctgcg cccgtcgtcg cggtagccgg cgctgggtat ccccgcgaac ttgaacacca
    29821 tggcgctgga cagggagtcg ggcagcaaca gaccccgcgc atgcacgtgg cgcgcctgtt
    29881 tgcgatgatt gctgaccgcg gcgcggtctt cgtgccagcg gcctttcatt tcaatgaagc
    29941 cggcgtggcc gaatccggcc agcagatcgc gcgcccaggg tttggcgcag acggtaacgg
    30001 ggatgtccgt gttgagcacc gtgcgcagac tgggcaggct catgcaaacg tcgccgatcc
    30061 agttggggag gcggatatag atggctgata tgatcggcat cgtggaaagc gccgcgctca
    30121 ggcgcggcat gggttcaagc ccgcattttt acccgatttt cgacaacggt aaaataggcg
    30181 cttcctcaga ctgtatcaaa gcgagcgtat ttttgaagcc atccccagcc tccgcaccgt
    30241 cgcatgcaga gccggtcaag ttcgatatct ggaaacggat ctactcacgg ctcggcccat
    30301 attggaaagc ggtggtgctg gccatggttc tgctcagcgt tgccgcggcc acgcaaccca
    30361 cgctcgccgt catcatgaaa ccgttgctcg acggcgggtt ttcgggtgcc aagccgtcat
    30421 atgtctggtc cattccactc gcggtcgtgg gcctgatact cctgcgcggt ctctgcggct
    30481 tcttcagcga ctatctgttg gcgtgggtgg ccaacaagat gctgctgggc atacgccgcg
    30541 aaatgttcga gcgcctgctg gggatgcccg acaccgattt caagcggggc gacacgggcc
    30601 gcctgctcaa tcgtttcacc atcgatgcca acaatgtgac cagctccgcc accgatgtga
    30661 tcactgtgtt ggtgcgcgaa tcgttgaccg tggtcgccct gattggcgta ttactgtaca
    30721 tgtcgtggca gcttacgctg atcgtgctgg tgatactgcc actgtctgtc gtcatttccc
    30781 gcatcttcac acgcaagctg cgtcgcgtga atcgtgacac ggtcaacatg aatgcggaac
    30841 tcacccgtgt ggtgggcgat ggcatcaatg ggcaacgagt aatcaaactg ttcgacggct
    30901 atgaatacga atcgggccga ttcgaataca tcaatgggcg gctgcgccgc ttcgccatgc
    30961 gtgcggcggt ggcgaacgcg gccatgtcgc cgctggccca gttcaccatt gccctgtctg
    31021 tcgctgtcgt catcgccgtc gcgttgtatc aggctaattc cggggtgttg acagtcggca
    31081 gcttcgcgtc gttcatggcg gcattgggtc agatattcga tccggtcaaa cggttgacca
    31141 acatctcggg ccgcatgcag cgcatgctgg tatcggccga gagcgtcttc accctcatcg
    31201 accacgaaac cgaggccgat accggcaaac tgtcactcga ccgagtgtcg gggcatatcg
    31261 agtttcgtga ccttgtccat cgcttcgagg atgcgtcgaa cgacacgctg tcgaacgtgt
    31321 cgttcgtggt cgaacccggc cagaccgtgg cgttggtggg gcgatccggc agcggtaaga
    31381 caacgctggt caacatgctc ccgaggttca tccatccgtc ttcggggcag gtgttgatcg
    31441 atggccagga catcgacgga ttcaccctgc gcagcctgcg gtcacaacta tcgctcgtca
    31501 gtcaggatgt cgtgttgttc gatggcacca tcggcgataa cgtgaactat ggtgcgttgc
    31561 acgacgccag ccaagacgaa attcgtggcg ctcttgaggc ggcgaatctg ctcgcgttcg
    31621 tggacagcct gcccaatggg gtggataccc ttgtggggga gaacgccaat ctgctttccg
    31681 gcgggcagcg tcaaaggctc gctatcgcac gggccttgat caagaatgcg cccatcctcg
    31741 ttcttgacga ggcgacgtcg gcgttggaca acgaatccga acgccaagtc caggcgtcac
    31801 tcgagcgtct catggctggg cgtaccaccc tggtcatcgc gcatcgcctc accaccgtgc
    31861 agaaggcgga ccggatcgtg gtgctcgatg aaggtagaat cgtcgaacac ggtcgccacg
    31921 acgaattgct ggcgctcgac ggtttgtacg cgtcgctcta tcgcatgcag tttcgggacg
    31981 tcgtgggcgc gtgacgcctg tcgcgccttg cccacgcgtc tggacaaaca ttttcacgaa
    32041 aggaacggga tcaccatgag aaaaccgctg ttgtgcctgg gggtcgtggt gttggccggt
    32101 tgtgccgcgc cgcacggcct gacgcgttac gtggatctgc atagtgaaga tcacctgcgc
    32161 gcgcagcggc acatcctgat ggattttccc aagatccaga tggccctgtt cgaacaccag
    32221 cgagcttgcg gttcggcgcc cgtgttcacc cgatcatcga aggaccccag ttatgccatg
    32281 atcacgcaaa aactgagccc gggcgccgac tggaaaaaca ccatagaggt ggaccttgcc
    32341 ttgttgggcg atctcaccgt ccaggccaag gcctatacct accatgctgg cttggaggcg
    32401 cagatcgatc aggtgttcaa cgccattgcg aagcccaaag tgtgcgccga acggaaaaaa
    32461 gccggatagc cacgccattc gagtattcga gttcggatgc cagggtaggc cgggcctaca
    32521 tcagcacaat gtcatattgc tcctgggaat attgggtctc cacctcgagc gagatcgcct
    32581 tgccgatgaa gtcgcccagc atggccaaat gcgtgctttc ctcttccagg aacaggtcca
    32641 cgatgccctg ggccgcgagg atgcggaatt cctttgggtt gaactgccgc gattcgcgca
    32701 ggatttcccg caggatctca tagcacaccg tacgtgccgt cttcacgctg ccgcgtgact
    32761 ggcaggtagg gcagggttcg cacagctggt gagccagtga atcccgggtc cgcttgcggg
    32821 tcatttccac caggccgagc tggctgaagc cactgacagt gatgcgggtt cgatcccggg
    32881 cgagcgcctt gcccagttca gccagcaccg atgcgcggtg tccggcatcg tccatatcga
    32941 tgaaatccag gatgatgatg ccgcccaggt tgcgtagacg aagctggcgg gccagcgcga
    33001 cggtggcctc caggttcgtc ttgaagatgg tgtcgttgaa attccggccg cccacgaacc
    33061 ctcccgtatt gacgtccacc gtggtcagtg cctcggtctg atcgatgatc aggtatccac
    33121 ccgatttgag attcacgcgt cgggacagcg cgcgggcgat ctcttcgtcc acgcccgcgg
    33181 tgtcgaacag cggccgatcg ccgctgtagt gattgatgcg ttcaagaacc gatggggtgt
    33241 attgcagtgc ccattcccgc agcgcgtgcg tggtcgtgcg cgaatcaacg aggatggtgc
    33301 tggtttcggg ccccaccatg tcgcgcagca cccgttgggc cagcgtcaga tcggtgtaca
    33361 gcgcggatgg cgcggcctgg cgccgggctt tgtcctgaat gctttgccag atggcgcgca
    33421 ggtacgtctg atcagctttc agctcgtcgt cggtggcgat ctcggcttgc gtgcgcacga
    33481 tatagccacc ggcctcattt tccggcatca ggttctgcac gcgttcacgc aactgggcgc
    33541 gttcctgctc ggatccgatc ttctgcgaaa tgccgatgtg cggctcgtgc ggcaggtaga
    33601 ccagcatgcg tcccgcgatg ctgatctggg tggacaaacg cgcgcccttg gtgcccagag
    33661 gatccttgat cacctgcacc atcagcgact gcccttcgaa aagcagtttc tctatgggcg
    33721 tcatgttgcc gccactgccc cgctcgccgc ggttctgctg cagatcggcc acgtggatga
    33781 aggcagcgcg ctcgagcccg atgtcgacaa acgcgctttg catccccggc agcacgcgca
    33841 cgattttccc aagataaatg ttgcctacat gcccgcgctg gatgctgcgc tcgacatgca
    33901 gttcctggac ggcgccttgc tctatcaagg cgacacgggt ttcaaaaggg gtgacgttga
    33961 tgaggatgtc ttcggtcatt ggggcgatgt cgataggctc ggggctccgc gtggggcccg
    34021 catgtattca gagagtagca cgcggtcgtc ggccatcaat ggcgtcaaca cgacggtttg
    34081 ctttgttgcg tgttatttgc tctcgttgct cttgctgtcc gtagcggtcg aagcgttcat
    34141 ggatttcgtg gagtgcgtgg gcgcgtggtg cttatggtga tcgagtagcg tcggcgggcg
    34201 ggcttcttgt gccgcgaccg agggtgggcc gaagcgcttc gacatccgat gaggcgttgg
    34261 gtgtgccaag aggttgtttg gccggatgtg gctgatgcca attgtcgcgg cttcagagcc
    34321 gatgcggggc ggcggcgtgg ggtgatatgg ggagggttga tacaggagcg cggtgctgca
    34381 agaagcgact gcggcgggac gtcacagaga aggagagggg ggtgaccggt agggcgattc
    34441 gattgatcgg gggggaaatg gaaatgtctg gcgcattgat ttgacacatc caaaggtggt
    34501 gtgttctaat tcttggctcg gtgattgatg aacgcggcgc taggagccgg gttgatcgaa
    34561 ggccggggcg gtcgagggtg gtggtgtttc aatgattgag cgcaccatgt ttggcggccc
    34621 gaagcgggta g
//
LOCUS       DASTNK010000107         3943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_818907, whole genome shotgun sequence.
ACCESSION   DASTNK010000107 DASTNK010000000
VERSION     DASTNK010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818907"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VFR20_08045"
     CDS             <1..724
                     /locus_tag="VFR20_08045"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEU0230508.1"
                     /translation="TALAASTHASPLIVLDPGHNPDHPGATSIQGTKEIRYNDRFVEE
                     LAPALEHAGWRVRLTRQPGQSIGLVERAELANRLHATLFLSIHHDSAQLRYLKKIRVG
                     NRTGYQTIKPIAGYSLYVSRENPDFAESLRLAELLGKQLRSLGRPPALHHAEKIPGEN
                     HPLLNKYLGIYQYDKLAVLRHTKVPAVLLEVGVITDKQDEAYVANAGNRKKMIGKIVE
                     ALQQYVHTEGAAKGATNGAKTQ"
     gene            complement(763..1776)
                     /locus_tag="VFR20_08050"
     CDS             complement(763..1776)
                     /locus_tag="VFR20_08050"
                     /inference="COORDINATES: protein motif:HMM:NF015226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HEU0230509.1"
                     /translation="MARMQTNAIQKGVGPGWATWVTRSPFLELLDTALRGSGQVIFMD
                     NPLTGALNFLALFWGAYAGGTTLAVAIGAMIGTLVATTTAYGLRVDRDILRKGLYGFN
                     GMLVGVGVPTFLGNTPIMWAVLVFACAASTIITLAVNNVVGKWNTPGLTFPFVLTTWL
                     VVLMAYRFPELRVIGMAPLAPPIGVAGGVFGLHDFVHASLVSVAQVYFVDNPVSGVLF
                     LLALLVESRWCAGLVAAGAMVAVGCALAMGADTSVISHGLWGYSAALTAPAIGCVYIK
                     TNAGTLAYCAVATLFTILVQGAMATLAPKLGIPALTFPFILTTWLFLIARYRLGAGSG
                     GAA"
     gene            2032..2388
                     /locus_tag="VFR20_08055"
     CDS             2032..2388
                     /locus_tag="VFR20_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230510.1"
                     /translation="MKIESFSWVADANHDGQVGLATVWHVLQWIYITPGNLLLEALGH
                     VHVTADLFHIQASEQTGYYSLNSWFAALFSLLFWLCVLVELCNLKDFIQARLRSRHPQ
                     SAPRHPHHHWPHLHRP"
     gene            2592..2927
                     /locus_tag="VFR20_08060"
     CDS             2592..2927
                     /locus_tag="VFR20_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HEU0230511.1"
                     /translation="MKRILLAGAAAVAMMCSTAAMAHVDVGISVGIPGLFFGAPGVVV
                     APPAYYPPPVYVAPPPPRVVVVPPPERIYTPVYGWRYPYRGYWRHHHWHGDHHWRGHR
                     VYHGHGDDD"
     gene            complement(3049..>3943)
                     /gene="serS"
                     /locus_tag="VFR20_08065"
     CDS             complement(3049..>3943)
                     /gene="serS"
                     /locus_tag="VFR20_08065"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU0230512.1"
                     /translation="LGLDFDAARKLAGARFMMLRGQMARLHRALAQFMLDLQTGEHGY
                     TECYTPYIVNGATLFGTGQLPKFKDDMFWVTKGGGESEPAEDGAARVADDLYLISTSE
                     ITLAGSMRDSITPQHDLPLKFTAHTPCFRSEAGSGGRDVRGIIRQHQFDKVEMVQITH
                     PGTSYDALEEMVGHAEAVLRRLGVPYRVVLLCGGDMGFGAAKTYDLEVWLPAQNTWRE
                     ISSVSNCEAFQARRMQARYRPDNGKPEYLHTLNGSGLAVGRALVAVLENHQREDGSVA
                     VPEVLRPYMGGLAELTPTGAT"
BASE COUNT          784 a         1283 c         1161 g          715 t
ORIGIN      
        1 gacggcgctg gcggcgtcca cgcacgccag cccccttatc gtgctggacc cgggccataa
       61 cccggaccac cccggcgcca ccagcatcca gggtaccaag gaaatccgct acaacgatcg
      121 ctttgtggaa gagctcgcgc cggcgctgga gcatgcggga tggcgggttc gattgactcg
      181 ccagcctggt caaagcattg ggctggtgga acgcgcggag ctcgcgaatc gtttgcatgc
      241 aacgctgttt ctatccatcc accacgattc cgcccaactg cggtatctga agaaaatcag
      301 ggttggcaat cgcaccggct accagaccat caagcccatt gccggttatt cgctgtacgt
      361 gtcgcgcgaa aacccggatt ttgccgaatc cctccggttg gccgaacttt tgggcaagca
      421 actgcgttcc cttggccggc cgcccgcgct ccatcacgca gagaagatcc ctggcgagaa
      481 ccaccccctg ctgaacaaat atctaggcat ataccagtat gacaagctgg cggtgttgcg
      541 gcacacgaag gtccccgccg tgctgctgga agtgggcgtg atcaccgaca agcaagacga
      601 ggcctatgtg gccaatgccg ggaatcgaaa gaagatgatc gggaaaatcg tcgaagcgct
      661 acaacagtat gttcacacag aaggtgccgc aaagggcgcc accaacggtg ccaagacgca
      721 atgatccggc acatgggttg tcggcgtgag ccacgccctg gctcacgccg ccccgcccga
      781 cccggcgcca agccggtaac gcgcgatcag gaacagccag gtcgtcagaa tgaaagggaa
      841 ggtcagcgcc ggaatgccaa gttttggcgc gagcgttgcc atagcccctt gcacgagaat
      901 ggtgaacaag gtggcgaccg cgcaataggc cagcgtgccg gcattcgtct ttatgtatac
      961 gcaccctatc gccggggcgg tcagcgccgc tgaatagccc cacaggccgt gggagatcac
     1021 gcttgtatcg gcccccatcg ccaacgcgca accaacggcg accatggccc ccgcagcgac
     1081 caggccggcg caccagcggg actccaccaa caacgccaac aggaaaagga cgccggacac
     1141 agggttgtcg acgaagtaca cctgcgccac gctcaccaac gaggcgtgga caaagtcatg
     1201 aaggccaaac actccgcccg ccacaccgat agggggtgcc aacggtgcca tcccgatgac
     1261 acgcagttcg ggaaaccgat acgccatcaa caccactaac cacgtggtca ggacgaaggg
     1321 gaaagtcaga cctggggtgt tccatttccc gaccacgttg ttgaccgcca gggtgatgat
     1381 cgtcgaggcg gcgcaggcga acaccagcac cgcccacatg atcggggtat tgcccagaaa
     1441 ggtcggaacc cccaccccga ccagcatgcc gttgaacccg tagagccctt tgcgcaggat
     1501 gtcgcggtca acgcgcaggc cgtaggccgt cgtcgtcgcg acaagcgtac cgatcatcgc
     1561 accgatcgcc accgccagcg tcgtgccgcc ggcataggcc ccccagaaca aggccaggaa
     1621 attcagggca cctgtgagcg ggttgtccat gaaaatgacc tggccgcttc cgcgcagggc
     1681 ggtgtccaga agctcaagga atgggctcct tgtcacccat gtggcccaac ccggcccgac
     1741 acctttttgt attgcgtttg tttgcatccg agccatatgt ccgttcagcc aacaccctgt
     1801 tcaacggtcg gattgtagcc ggcccggcac ctcccggcgg tggttggtgg gctcaacgtg
     1861 gcaagccgtc ggttgggccg gcttgccatt ctcctcgagc caccttgcct ttccccttcg
     1921 gcacgcctat agtgaaattc aaggatcgaa ttcgaactgg tattgggggg tacgcgcccc
     1981 gtactctcct ggcccgggcg gcatcagccg gcatgtcaaa ggagttctgc catgaagatc
     2041 gaatcgttca gctgggttgc cgacgccaac cacgatgggc aggttggctt ggccacggtg
     2101 tggcacgtgc tgcagtggat ctacatcacg ccgggcaatt tgttgcttga agcccttggc
     2161 cacgttcacg tcaccgccga cctgttccac atccaggcgt ccgaacaaac gggctactac
     2221 agcctgaaca gctggttcgc ggcattgttc tcgctgttgt tctggctgtg tgttctggtg
     2281 gagctctgca acctgaagga cttcatccag gccaggctgc ggagcaggca cccgcaatcc
     2341 gcgccgcgac acccccacca tcattggccc catctgcaca ggccatgacg ggctgtggct
     2401 gtggaactca aagccccgaa aaccctgaaa cacgttgaaa tttccggcga aatgggtaat
     2461 gaaacgtaaa tagcccgtgc gaaaaaccgt ccggcgttgc gttttgatgc tttttcgaac
     2521 ggcccttctt acatgtgccg cccgaaatcc cgccacaatg gaatcgtcaa agtgaataac
     2581 aggtacaaga catgaaacga attctgcttg ctggcgccgc ggcagtcgcc atgatgtgtt
     2641 ccacggccgc catggcccac gtcgacgtcg gcatctcagt aggcattccc gggcttttct
     2701 tcggcgcgcc aggtgtcgtt gtggcgccgc ctgcttatta cccgccgccc gtgtacgtgg
     2761 cgcctccccc gcctcgggtg gtcgtcgtgc cgccccccga acgcatctat accccggtct
     2821 atggatggcg atacccgtat cgcggctatt ggcgacacca tcattggcac ggcgaccatc
     2881 actggcgtgg ccacagggtg tatcatggtc acggcgatga tgattgagcg cagcatcgct
     2941 tctcatgtga aatctcctcc tgatgtgcag tggtaaaaga aggggccgcc ccattggggc
     3001 ggccccttct tccatgcgcc gcaagcgccg tgtatcaacc acccggcttc aggtggcccc
     3061 ggtcggcgtc agttccgcca ggccgcccat gtagggccgc agtacttcag gcaccgccac
     3121 gctgccatct tcccgctgat gattttccag cacggccacc agggcacgcc ccacggccaa
     3181 ccctgaacca ttcaacgtgt gcaggtattc cggcttgccg ttgtcggggc ggtagcgggc
     3241 ctgcatgcgg cgcgcctgga acgcctcaca gttggaaacc gaagagatct cgcgccaagt
     3301 gttctgcgcc ggcagccaga cctccagatc gtaggtcttg gcggccccga atcccatgtc
     3361 gcccccgcac agcagcacga cccggtatgg cacgcccaag cgccgcagca cggcctcggc
     3421 gtggccaacc atttcctcga gcgcgtcgta ggacgtcccg gggtgcgtga tctgcaccat
     3481 ctcgaccttg tcgaactggt gctggcggat gatgccacgc acgtcgcgcc cgccgctccc
     3541 ggcctcggag cggaagcatg gcgtgtgggc ggtgaatttc aggggaaggt cgtgctgggg
     3601 cgtgatgctg tcacgcatgg agcccgccag cgtgatttcc gacgtggaga tcaggtacag
     3661 gtcgtccgcc acgcgcgccg cgccgtcttc ggcgggctcg ctctcgccgc cgcctttcgt
     3721 gacccagaac atatcgtcct tgaacttggg caactgaccg gttccaaata gcgtggcacc
     3781 gttgacgata tacggggtgt agcactcggt gtaaccatgt tcgcccgttt gcaggtcgag
     3841 catgaactgc gccagcgcgc gatgcaaccg ggccatctgg cctcgcaaca tcatgaaacg
     3901 cgcccctgcc agcttgcgtg ccgcgtcgaa atccagcccc agg
//
LOCUS       DASTNK010000108        33476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_79111, whole genome shotgun sequence.
ACCESSION   DASTNK010000108 DASTNK010000000
VERSION     DASTNK010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 33476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33476
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79111"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /gene="rlmD"
                     /locus_tag="VFR20_08070"
     CDS             <1..1164
                     /gene="rlmD"
                     /locus_tag="VFR20_08070"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU0230513.1"
                     /translation="VVRPSSQRTTPPCPNFGVCGGCAMQHLDPAAQVAVKQRALEDSL
                     WHIGKVRPERILPALHGPTWGYRHRARLSVRLVQKKGGVLVGFRERHSSYVVDMTECL
                     VLPPHVSALLRPLRMLVGALSAPNRIPQIEVAAGDDSVALLMRHLEPLSDADLRLLAA
                     FGDEHGVSWWLQPGGFESVHPLRPEEEHTLAYTLPEFGLRMPFRPTDFTQVNYAINRA
                     LVSKALQLLGVRETDRVADMFCGLGNFSLPLALRSREVVGIEGSGILTRRAQATAAAC
                     GLAEKTRFETCNLFEADAEWLRGLGAFDRMLFDPPREGALALSSAVSSIPAHERPRRI
                     VYVSCNPATLARDAGVLVHAAGYSMTSAGVVNMFPHTGHVESIAVFDATPQAA"
     gene            complement(1205..2644)
                     /gene="argH"
                     /locus_tag="VFR20_08075"
     CDS             complement(1205..2644)
                     /gene="argH"
                     /locus_tag="VFR20_08075"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU0230514.1"
                     /translation="MPNNTPTPSSDPSHDQFANKAKAWSARFSEPVSDLVKRYTASVG
                     FDQRMAHCDIDGSLAHADMLAAVGVISADDLTAIQRGMARIRQEIDSGDFRWSLDLED
                     VHLNIEKRLVELVGDAGKRLHTGRSRNDQVATDIRLWVRAEIDTCVGLLRQLRRNLAT
                     LALEHAATILPGFTHLQVAQPVTFGHHLLAYAEMFGRDIERLADCRHRVNRLPLGAAA
                     LAGTSYPIDRERVARTLGFDGVCRNSLDAVSDRDFAIEFCADAALIMVHVSRLSEELV
                     LWVSPRVGFIELADRFCTGSSIMPQKKNPDVPELARGKTGRVNGHLMALLTLMKGQPL
                     AYNKDNQEDKEGLFDTADTIRDTLTIFADMVGGIRVKAEAMRAAALQGFATATDLADY
                     LVKKGTPFRDAHETVALAVRTCEERGCDLADLPLEQLRAFHPSIDADVYEVLTLEGSV
                     ASRKHIGGTAPERVKLEAERILAESANQA"
     gene            2758..4374
                     /locus_tag="VFR20_08080"
     CDS             2758..4374
                     /locus_tag="VFR20_08080"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEU0230515.1"
                     /translation="MTTVRIGLAQINTAVGNLPANAARILDAARWASEHRADALLTPE
                     LGLTGYPAEDLLIRPAFIDRQLDVLDRLRGDLARFRGLHVIVGHVRRDGNRLYNSASV
                     MADGRVVATYDKHELPNYAVFDEKRYFAPGAKPAVFDLRGLKCGVGICEDTWFPDSPA
                     KAAARDAKVWLVLNASPFTLAKQDERIRQAATNAQGMATVYLNKVGGQDELVFDGASF
                     VLDARGRVVKRLPVFEECLDLVEFDSLGEPALAESPAQWPDEPEQVWRALVLAVRDYL
                     AKTGFSKVVLGLSGGIDSAVVLAVAVDALGADKVRAVMMPSRYTADMSLADARQMADT
                     LGVAYDEIGISSLATAFDQALAPQFAGLPLDATEENIQARIRGILLMALSNKTGALVL
                     STGNKSELATGYCTLYGDMAGGFAVIKDVPKTLVYRLARWRNGVSSVIPQRIIDRAPS
                     AELRPDQTDQDSLPPYDVLDAIVERYVEHNEPRDDIVRSGFDADVVDQILRLIRINEY
                     KRRQGAPGPKITTRAFGRDWRFPIANGFRE"
     gene            4451..4789
                     /locus_tag="VFR20_08085"
     CDS             4451..4789
                     /locus_tag="VFR20_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEU0230516.1"
                     /translation="MKQVTAIIKPFKLDEVREALAEIGVSGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKIRIDVALADDMVESAIDAIVKAARTGKIGDGKIFVTPVEQAIRI
                     RTGEIGAEAL"
     gene            5134..6036
                     /locus_tag="VFR20_08090"
     CDS             5134..6036
                     /locus_tag="VFR20_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0230517.1"
                     /translation="MKLLPLVLATAVTAVGMTAAQAADSRIDKIKSTGEITLGYRESS
                     IPFSYLDDNQKPIGYSMDICYGVVDALKKDLKMPNIKVKLVSVTSSTRIPLVANGTID
                     LSCGSATNNAQRQVQVSFAPTTFVTATRFTALKKSHVNDLKDFKGKTVVSTSGTTNLK
                     WLTQTNADEHLGMRIIPAKDHSEAFLTVASGRASAFFMDDILLAGLVANSRNPGEWMI
                     SKHAYTIEPYGIIEPKGDPQFKTAVDDAVKAMMKDGKLDQLYAKWFTKPIPPKNINLN
                     WPMTPELKKAFAHPTDSPDPAVYK"
     gene            6156..6896
                     /locus_tag="VFR20_08095"
     CDS             6156..6896
                     /locus_tag="VFR20_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU0230518.1"
                     /translation="MNYHWNWMIFWAQAPDGTGTYMHTLIRGMYWTLGVSILAWIIAL
                     VFGVMVGVFRTTRIGWLERLCAGYVELFRNVPLLVQMFLWYFVFPEVVPSAIGNWIKA
                     ADPTWSTFWTAVVCLGLFTSARIAEQVRAGVTSLPRGQNMAGTALGLTRPQVYRYLLL
                     PMTVRIIMPPLTSEALNLIKNSSVAFTIGLLEITGAARSMQEFSFQIFESFTAATVLY
                     LIINAIVVVLMRYIEKWISVPGFIGTKR"
     gene            6907..7596
                     /locus_tag="VFR20_08100"
     CDS             6907..7596
                     /locus_tag="VFR20_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU0230519.1"
                     /translation="MHGFDFEVIWRTLPYVFGTGMVFTLKLTILSTIGALILGTLLAM
                     MRLSSNRFIAGVSGGYVNFLRALPLILIIFWFYFLVPYIFGWITRAGHPISVGGFTSA
                     LITFVLFEAAYFCEIMRAGIQSIPRGQIGAGYALGLNYWQVMGHIVLPQAFRNMTPVI
                     LTQMIVLFQDTSLVYVLSLTDFLGAANNVAQRDGRLVEMFIFAAVVYFLISYAASRSV
                     KHLERRFAIVR"
     gene            7612..8346
                     /locus_tag="VFR20_08105"
     CDS             7612..8346
                     /locus_tag="VFR20_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU0230520.1"
                     /translation="MIEFKNVSKWYGAFQVLTDCSTSVSKGEVVVVCGPSGSGKSTLI
                     KTANALEPFQKGEIFIDGVSVGASSTNLPKLRSRIGMVFQHFELFPHLSVLRNLSLAQ
                     IKVLKRPEAEAVKRARALLERVGLSDHINKYPGQLSGGQQQRVAIARALAMDPIAMLF
                     DEPTSALDPEMVNEVLDVMVDLAKEGMTMMVVTHEMGFAKKVADRVIFMDAGKIVEDC
                     SKDDFFDHPDQRSDRAKQFLSKILSH"
     gene            8473..8892
                     /locus_tag="VFR20_08110"
     CDS             8473..8892
                     /locus_tag="VFR20_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase subunit alpha"
                     /protein_id="HEU0230521.1"
                     /translation="MSDTDPPLTDFASLLVLAYKQEEPQRMLFVFARKELPEHASIGQ
                     RERFNNGEGGGLRPCLCVDKALEEISSFESLVAESAATGQSWDVAFVGCLAGRGGIPP
                     NPDEADYPLRLMVNAINNGRVSDLAAFDRNGRALRFI"
     gene            complement(8885..9454)
                     /locus_tag="VFR20_08115"
     CDS             complement(8885..9454)
                     /locus_tag="VFR20_08115"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEU0230522.1"
                     /translation="MDPEAINPGNWHELAPPSLTHEQKHWLLRPGALTAGLRQLGHVD
                     LTVVSEYTDGLSAREAWMVRRPPGSAMHIREIKMAINGVDSVVARSFTPRNASFSWWR
                     GMRALNRRPLADMLYHNPQITRSRFFVCRLRPSQPLYGAVKRALVTPTPTAHELFARC
                     SVFWRHGYPLLVAECFLPGFWPLTAGRPK"
     gene            9491..10225
                     /locus_tag="VFR20_08120"
     CDS             9491..10225
                     /locus_tag="VFR20_08120"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU0230523.1"
                     /translation="MEKVRISKLMAERGLCSRREADAYIEKGWVRVDGIVVSELGSKA
                     LPSQTITLERQAREKQVARVTILLNKPVGYVSAQAEKGYRPAVSLIDAASQYHADASR
                     LRFDRSHLRGLAPAGRLDIDSQGLLVLTQDGRIARQLIGDDSSVDKEYLVRVQGRLAA
                     SGLALLNHGLTLDGRPLKPAQVTWQNDDQLRFILREGKKRQIRRMCELVGLRVVGLKR
                     VRMGRVVLGDLPEGKWRYLKPGERFI"
     gene            complement(10342..11199)
                     /gene="rlmJ"
                     /locus_tag="VFR20_08125"
     CDS             complement(10342..11199)
                     /gene="rlmJ"
                     /locus_tag="VFR20_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017425060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEU0230524.1"
                     /translation="MFSYRHAFHAGNHADVLKHAIFVHILDYFNQKESPYWVVDTHAG
                     AGIYDLADDWAAQNAEYVDGLDRLLGAPDMPALVENYLRAVQDHNPDGVANFYPGSPW
                     LALRALREQDRLHLFEKHPTEVDVLRKNLAAMGQLARKQTQIHDADGFDGLKALLPPL
                     TRRGVVIIDPSYENKADYRRTLNAVNDGLKRFATGCFMVWYPLVQRVESLDLARSLER
                     LPAKSWLHASLSVRKPASDGLGLHGSGVFVVNPPWTLHAALEQALPWLRQRLGQDDRA
                     RFTLNQKTS"
     gene            complement(11220..11972)
                     /gene="ugpQ"
                     /locus_tag="VFR20_08130"
     CDS             complement(11220..11972)
                     /gene="ugpQ"
                     /locus_tag="VFR20_08130"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU0230525.1"
                     /translation="MSDTSFIWPYPRVIAHRGGGHFAPENTLASMRLGAQRGFTMVEY
                     DVKLTSDDVAVLLHDDKIDRTSNGSGDAGAMTYRELATLDFGAWHSVRYAGEPIPTLH
                     AIAAFTQHNNVLSNIEIKPHTGTDDHTGTLVARMARLMWVHAQVPPLMSSFSEIALAA
                     AQREAPELPRALLIEGELPPDWRDRLRRLGCVALNMNQRFVTPDIINHVHETGCKITA
                     WTVNDTVRARDLLDWGCDAIFTDEIEAIPPSI"
     gene            12138..14111
                     /locus_tag="VFR20_08135"
     CDS             12138..14111
                     /locus_tag="VFR20_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEU0230526.1"
                     /translation="MFDFIRSHQRLMQVILLVLILPSFALIGVSGYSTYVSGDHDMVK
                     VGGHAITLQDYDQARRNQLRELQSQNRAGFDPSSLDNEAARSALLESMIDRRVIATVA
                     SDDRFSVSDAVLRQAIAAIPQLQVNGEFSSERYRDLLKSMGMTSRDFERGQRAELALG
                     RVLGPVDTSATLPRPVTDSIERALTAQRTVRLRIYPVSNYEKDVKVSDADIKSWYDGN
                     KQQLKLPEQVDIQYLLLDEAAAMKNVPAVSPDAEKKYYEQNKSRFVSPARVNLSHILV
                     SVPVGATEAQRDAALKKARKIDTEVQASKSQFADIARKQSQDAGTARQGGELGWITQG
                     SWPANLDKVVFALKKGEVSGVVDGPGGYHIFKANDVDPSKTQSFEDAKPQVEKEIRQQ
                     LAADKFADMSTRLTSLVYDNASSLQPAADALGLQVKTASGIARERLLDANEAGSGAAA
                     TSPDAAVLADVRVRRALFTPRVLTDKQNSGVIEISPDTLIVVRVAKSIPAHIPPLDKV
                     TATIRKTLIDKKALADAVKAGQAAVAGYRKAGDSAKVPDGFGSAMAVSRIDPQGLSKP
                     VLDAALAASVKTLPTYAGMQAPTAYVVIRIEKAEPGKAGDTELAGLPAQLDQLWGNAE
                     KAAVLKALRQEVKVKMLPESKKVLSGETENQDS"
     gene            complement(14541..16835)
                     /locus_tag="VFR20_08140"
     CDS             complement(14541..16835)
                     /locus_tag="VFR20_08140"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEU0230527.1"
                     /translation="MDAKADLAKLALDYHAYPTPGKISITPTKPLANQDDLSLAYSPG
                     VAYACMAIHADGPEAAAKYTSRSNLVGVITNGTAVLGLGNIGPLAAKPVMEGKGCLFK
                     KFAGIDVFDIELNENDPDKLVDVIAALEPTLGGINLEDIKAPECFYIEKKLRERLKIP
                     VFHDDQHGTAIISSAAILNGLKVVDKNIGDVKLVCSGAGAASIACLDLLVHLGVARAN
                     IFVVDSRGVIWKGRDQNMEPNKARYAQDTDARTLADVCRDADVFLGCSAPGVLTPDMV
                     KTMAPRPLILALANPEPEIRPEAAKAARSDCIVATGRSDYPNQVNNVLCFPFIFRGAM
                     DVGATVINEEMKLACVKAIASLAEAEQNDEVASAYVGQELVFGPDYIIPKPFDSRLII
                     KIAPAVAQAAMDSGVATRPIEDMEAYRQRLMSIVYHTGQLMRPLFMQAKADPKRVIYA
                     DGEDERVLRAVQTVVDERIARPILIGRPSVIEMRVRKFGLRLDLASDLEIVNPEDDSR
                     FHDTWTAYYKLQGREGVTPEIAKAMVRKHNSLIGVMLLQRGDADALLCGAASRYDNQL
                     KYVQEVIGLKPGARTYAAMNVLMLPTQTLFICDTHVNENPDAEQIADMTIQAAREVLR
                     FGVTPKVALLSHSNFGSRPTESSRKMARARQIIAERAPDLEVDGEMHADSALSESIRL
                     KAYPDSSLKGPANLLITPNLDTGNITYNMLKMTGSHGVAMGPVLLGAARPVHVLTTSA
                     TVRRIVNMTALAVVDAQQEASQQR"
     gene            17258..20128
                     /locus_tag="VFR20_08145"
     CDS             17258..20128
                     /locus_tag="VFR20_08145"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEU0230528.1"
                     /translation="MSSVSELKSNSYLFGSNAPYVEELYESFLDDPGSVPDKWRDYFD
                     QLQHTPATDGSEITRDQAHAPIIESFAQRAKSNGFAVRVKAPDLAVAGKQVYVQSIIA
                     AYRSLGSRFAQLDPLKRHDRPNIPELDPGFYGLTEADLDQVYSATNTYFTKADTMTMR
                     DMLKALRDTYCRDVGAEFMHISDPACKRWIQERLESTMGVPAYSAERKRHILQQVTEA
                     EGLERFLHTKYVGQKRFSLEGGESFIASMDEVVNHAGDNGVQELVVGMAHRGRLNMLV
                     NIMGKMPGDLFAEFEGKHAEGLSDGDVKYHNGFSSDLSTRGGPIHLSLAFNPSHLEIV
                     NPVVEGSVRARQDRRADVHGSEVLPVLVHGDAAFAGQGVVMETLNLAQTRGYGTGGTL
                     HIVINNQIGFTTSDPRDTRSTLYCTDVVKMIEAPVFHVNGDDPEAVVYVTSLALDYRR
                     EFHHDVVVDIVCFRKLGHNEQDTPSLTQPLMYKSIGKHPGTRKVYADKLVAQGVLAEG
                     EPDQMVKDYRQIMEDGRRTVEPVLTDYKNKYATDWSAFLNAKWTDQADTGVPLSELTR
                     IGERITTIPDGFTLHPLVSKLLNDRRNMVRGEHKLDWGMGEHLAFATLLSSGYAIRIT
                     GQDSGRGTFSHRHAVLHDQKRERWDDGTYIPLQNVSDTQASFTVIDSVLSEEAVLAFE
                     YGYATAEPNVLTIWEAQFGDFVNGAQVVIDQFITSGEAKWGRQCGLTLMLPHGYEGQG
                     PEHSSARVERFLQLCADNNIQAVQPTNGAQIFHVLRRQMIRPFRKPLVIFTPKSLLRN
                     KDASSDLDDLVNGRFLPVIGELDAAIDPDGVKRVLVCSGKVYYDIVHARAAAGRTDVA
                     VLRVEQLYPFAHKAFQTELLKYKNARELVWVQDEPQNQGAWFYVQHHLYENMAEGQKL
                     GYAGRPASASPAVGYLAKHQEQQKTLLEQALAPKFKGYMLAK"
     gene            20160..21365
                     /gene="odhB"
                     /locus_tag="VFR20_08150"
     CDS             20160..21365
                     /gene="odhB"
                     /locus_tag="VFR20_08150"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEU0230529.1"
                     /translation="MAITEVVVPQLSESVSEATLLNWKKQPGEAIEADEILIEVETDK
                     VVLEVPAPVSGVLSEIVKGDGSTVTSGEVLARIDTEAKAAAPAAAPAKAAPAAPAPAA
                     ATAAPVASAAASPAAAKILAEKGVDPASVEGSGRGGRITKGDALQAGGQPAPKAAAPV
                     APPTLSLDGRPEQRVPMSRLRARVAERLLQSQSENAILTTFNEVSMQAILDLRSRYKE
                     KFEKEHGIKLGFTSFFVKAAVAALKKYPVVNASVDGKDIIYHGYFDIGVAVGSPRGLV
                     VPIVRNADQLSIADIEKQIADFGARARDGKLAIEELTGGTFSISNGGVFGSMLSTPII
                     NPPQAAILGIHATKDRAVVENGQIVIRPMNYLALSYDHRIIDGREAVLALVAMKEALE
                     DPQRLLLEL"
     gene            21547..22974
                     /gene="lpdA"
                     /locus_tag="VFR20_08155"
     CDS             21547..22974
                     /gene="lpdA"
                     /locus_tag="VFR20_08155"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU0230530.1"
                     /translation="MAKQFDVVVIGGGPGGYVAAIRAAQLGLSAACVDAWSNEQGKPA
                     LGGTCTNIGCIPSKALLQSSEHYEQAGTHFADHGIEVQGLKLDLGKLLARKSTVVKQN
                     NDGIQYLFKKNKVTFFHGTGAFSAQVDGGWSIKVSGSSDEELVARHVIVATGSTARAL
                     PGLPFDENVVLSNDGALSIDPVPKKLGIIGAGVIGLEMGSVWRRLGAEVTILEAMPDF
                     LAAVDQQVAKEAQKVFAKQGLVIHTGAKIGEVKATAKSVTVPWTDASGAEQKLVVDRL
                     IVSIGRLPNTTGLGADVVGLKLDERGFVAVDDDCRTNLPNVWAVGDVVRGPMLAHKAE
                     EEGVAVAERIAGQHGHVNFGTIPWVIYTTPEIAWVGKTEQQLKTEGRDYRAGSFPFLA
                     NGRARALGDTTGFVKVLADAKTDEVLGVHIIGPVASELISEAVTIMEFRGASEDIARI
                     CHAHPTLSEAVKEAALAVDKRALNF"
     gene            23039..24130
                     /gene="zapE"
                     /locus_tag="VFR20_08160"
     CDS             23039..24130
                     /gene="zapE"
                     /locus_tag="VFR20_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEU0230531.1"
                     /translation="MNVREYYQNALDERGYQSDEAQLAAVERLQRYYDDWVAFKRARS
                     TTLRKIFRRPDVPRGVYLWGGVGRGKSFLMDAFYLTVPVRRKTRIHFHEFMRGVHREL
                     DEVRGQADPLDVVATRVAKRYRLICFDEFHVSDVADAMILHRLLLRLFELGTSFVMTS
                     NYEPSALYPDGLHRDRILPAIKLLEERMDILNVDTGIDYRRRTMAQVRTYLTPIDDAT
                     RDELRENFRRLSDTAPQKPVLHIEHRDIKAVALGGSIVWFDFATLCGGPRSQNDYLEL
                     ASRFRTVILSDVPRMDARNASEARRFTWLIDVFYDHRIKLIVSAQCPPEELYTRGPMA
                     NEFQRTVSRIMEMQTLEYLEAERRQAVVL"
     gene            24206..25537
                     /locus_tag="VFR20_08165"
     CDS             24206..25537
                     /locus_tag="VFR20_08165"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU0230532.1"
                     /translation="MNTRVLTGITTSGTPHLGNFAGAIRPAIQTSTHPDVDAFFFMAD
                     YHALIKCDDPARISRSRLEIAATWLAAGLDADKVTFYRQSDIPEIPELCWMLTCVTAK
                     GLMNRAHAYKAAVDQNMARGEEPDDGVTMGLFSYPVLMAADILMFNANKVPVGRDQIQ
                     HLEMARDIAQRFNHLYGKGVDYFVLPDVQIDDEVATLPGLDGRKMSKSYDNTIPLFEG
                     GAKATRDAVMRIVTDSRKPGEPKDAEGSALYTIYRAFAQPEESARFRRELEDGMGWGD
                     AKDALCRRIEAELGAMREQYASLMAHPDRIEDILQAGADKAKRLSTPFMQRLREAVGL
                     VRGRASRPVAEAGKKGERRARFVSFRDDDGGFRFRLLGSDGAELMLSRPFGDPRQAGL
                     AMRRLRDESLDALSIERDGVVTVVVDDSPIGEFDSALRERVWQALAELADK"
     gene            complement(25642..26490)
                     /locus_tag="VFR20_08170"
     CDS             complement(25642..26490)
                     /locus_tag="VFR20_08170"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0230533.1"
                     /translation="MKKQPPRFPPYVSRIEKAWFHVQRYYCVLILLFLLLPILVIMPL
                     SFSESSLLLYPIHDFSFKWYHTLFHSDDWIRAAKNSFIVAPSATLIATILGTLAAAGL
                     HRTEFRGKGLLMAVLISPMIVPLVVVGVGIYLFFAPYGLTSSYIGLILAHAALGAPFV
                     VTTVAATLQGYDQNLTRASHSLGAGPVRTFFGITLPIIAPGVVSGALFAFSTSFDEVV
                     VTLFLAGPEQVTLPRQMFTGIRENISPVIAAVATILIVFSVLLLLTLEWLRGRAHART
                     IAPDAA"
     gene            complement(26495..27787)
                     /locus_tag="VFR20_08175"
     CDS             complement(26495..27787)
                     /locus_tag="VFR20_08175"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0230534.1"
                     /translation="MTATLSETDTHAIRLAMRRAERRRKLRAFLLVAPLALFLLVIFV
                     IPIGALLERAVANPEVIHNLPRTLVALKSWDRTSTPPSVAFEALGADLGKVRNTANAG
                     TLARRLNYEIPGYRSLLFKTMRHLGADDAPAAQPLAASNTTASTPDPSQAIKDRFLHI
                     DKRWSQPEYWRAIIDNDSRYSSYYLLASMDLKRDMNGHIVHVPAGSSAFRDIYGRTFV
                     ISLVVTLLTLVLGYPLAYWISKLPRRTANLVMICVLIPFWTSILVRIAAWIVLLQRQG
                     LVNKALMSVGLTNTPFDLLFNRTGVYIAMTHILLPFMLLPIYSVMQSVPNTYQRAAIS
                     LGSHPFAAFWRVYVPQTYPGIGAGTLLVFIIAVGYYITPALLGGASDQMISYYVAYFT
                     NQTINWGMASALGFILLLGTLLLYSLYRRLSGRELGLG"
     gene            complement(27858..28907)
                     /locus_tag="VFR20_08180"
     CDS             complement(27858..28907)
                     /locus_tag="VFR20_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0230535.1"
                     /translation="MKTSKTLCSLAIVSAIAAMGVGTAHARDLTVVNFGGANGDAQKV
                     AFIKPLEKKMGKNIIAVAYNGEQAKVKAMVDTHNVVWDAVEVETGDLGRGCDEGLYEK
                     LNWDHIGKKSDFIPAAVRECGIGAFVWSTVLAYNGKTLKTAPKSWADFWNVKKFPGKR
                     GMRKGARYNLEFALMADGVAPKDVYKVLGTPEGVNRAFKKLTELKPYIQWWEAGAQPP
                     QMLAAGDVVMSTAYNGRISAANKDDHQSLKTVWNQNIYDLDYWVIPTGSPNKALAEQF
                     IKFATSPGPQAAYAEQISYGPVNKHAIGKLSPKILAELPNSPENGKNALLSSYNFWAD
                     HGEELEQRFTAWSTQ"
     gene            complement(28986..30077)
                     /locus_tag="VFR20_08185"
     CDS             complement(28986..30077)
                     /locus_tag="VFR20_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0230536.1"
                     /translation="MTPSEPLVSFRGVQKSYDGEALVVKNLNLDIRPGEFLSLLGPSG
                     SGKTTCLMMLAGFEFPTAGDIFLDGQRLNDVPPHKRNIGMVFQNYALFPHMTVEKNLH
                     YPLKVRHVPTAERAKRVAEALAMVQMSAFAKRYPGQLSGGQQQRIALARALVFDPKLV
                     LMDEPLGALDKQLREHMQLELKALHRSLGVTFVYVTHDQSEALTMSDRVAVFDQGVIQ
                     QISPVRTLYEEPQNRFVAGFVGDCNQIDGVIESEQDGRCQVRLPDDTLITALNIGALR
                     VGAACVLAIRPERLRIADGSQTTNAIRVQALDQVYFGDHIRLRCQALESTTLFVKLPL
                     DQKDALGRARAGDSILLSIPTEHARVFSP"
     gene            complement(30125..31390)
                     /gene="gabT"
                     /locus_tag="VFR20_08190"
     CDS             complement(30125..31390)
                     /gene="gabT"
                     /locus_tag="VFR20_08190"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEU0230537.1"
                     /translation="MDNQSWQTRKSNATPSGIGILCDFYTERALNAELWDVQGRRYID
                     FAAGIAVTNTGHCHPRVVAAISQQLSKFTHTAFQISPYSSYVELAEKINERAPGDHQK
                     KTALFTTGAEAVENAVKIARAATGRSGVIAFSGAFHGRTFMGLALTGKVAPYKLGFGP
                     FPGDVYHAPFPNALHGVSVQDSLDAIAALFKADIDPRRVAAIIVEPVQGEGGFYVAPP
                     EFLRALRALCDQHGILLIADEVQTGFGRTGKMFAMEHSGVIPDLMTMAKSLAGGMPLS
                     GVCGRAEIMDAPAPGALGGTYAGNPLAVASALAVLDIIDSEKLVERANIMGAKLRTTL
                     NDLAASTPAIAEVRGLGAMIAVEFHHPDDRRPNPELAASIQKRALQNGLVLLTCGVHG
                     NVIRFLFPLTIEDTVLDEGLHILSEAIRS"
     gene            31502..33019
                     /locus_tag="VFR20_08195"
     CDS             31502..33019
                     /locus_tag="VFR20_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU0230538.1"
                     /translation="MSSGFFTLADWLQQWLDVGTGEPAYRQLYRLIRGAILDGHLGAG
                     VRLPSSRALAVDLSIARNTVLQVYEQLEIEGYVTAGTGRGTFVVDISQEMCNDAKHSA
                     PRIAPPGTSTRGLSPRGRKLVEQAGFSNRQWGAFMPGVPDVAEFPSKVWNRIQSRHWR
                     RAPTELLTYAPAGGYEPLRHEVANYLNSARSVNGGAGQVVMTTGIHQGVDVVSRLLCQ
                     PGDLVWIEEPSYWGIRNLLLSSDMEVVSVPVDGEGMCLSAADLSRPPRLIVVSPSHQY
                     PLGMVMSLARRRMLLEYARQNGCWIIEDDYDSEFRFGSRPLPSLQGLDDSGLVLYAGS
                     FSKTLYPGLRVGYLVVPGPLAGPFANAIAELYREGQLITQSILAEFIREGFLSSHIRR
                     VRNLYAERRVALIAAVSRHFGNDLHIYGGEAGLHLVLGLPPEVDDVLVAREAFVRGVI
                     ARPLSRYYMNPSHVRQGLLLGYASVPLDRIDAAFDVLATVIQRHLLAASGRSVNA"
BASE COUNT         6220 a        10020 c        10713 g         6523 t
ORIGIN      
        1 gtcgtgcggc cctcgtcgca gcgcaccacg ccaccctgtc ccaatttcgg cgtctgcggc
       61 ggctgtgcca tgcagcacct cgaccccgcg gcccaggtgg ctgtcaaaca gcgcgcgttg
      121 gaagactcac tctggcatat cggcaaagtg cggcccgagc ggattctgcc cgcgctgcat
      181 ggccccacct gggggtaccg gcaccgcgcc agactgtcgg tgcggctggt gcagaagaag
      241 ggcggcgtgc tggtggggtt tcgggagcgg cacagcagtt acgtcgtgga catgaccgag
      301 tgcctcgtgc tgccgccaca cgtgtcggcg ctgctgcggc cgctgcgcat gctggtgggc
      361 gcgctgtcgg cgcccaaccg cattccccag atcgaagtcg cggcgggcga cgactcggtg
      421 gccttgctca tgcgccatct cgaaccgctg tccgacgcgg accttcgtct gctggccgcg
      481 ttcggtgacg agcatggtgt ttcatggtgg ctgcagccag gcgggttcga atccgttcac
      541 ccattgcgac ccgaagaaga acacaccctg gcctacaccc tgcccgaatt cggcttgcgc
      601 atgccgttcc ggcccaccga tttcacccag gtcaattatg cgatcaaccg cgcgttggtc
      661 agcaaggcgt tgcaattgct cggcgttcgc gaaaccgacc gggtggccga catgttctgc
      721 gggctgggca atttcagtct gccgctggcc ttgcgctcgc gcgaagtcgt cggcatcgag
      781 ggcagcggga tattgacccg ccgcgcacag gccaccgcgg cggcttgcgg cctggcggag
      841 aaaacccggt ttgagacctg caatctgttc gaggccgacg ccgagtggtt gcgcgggctg
      901 ggggccttcg accgcatgct gttcgatccg ccacgagagg gcgcccttgc gctgtccagc
      961 gcggtatcaa gcatccccgc ccacgaacgc ccgcgccgaa tcgtctacgt ctcgtgcaac
     1021 cccgccacgc tggcacgcga cgctggtgtt ttggtgcacg cagcgggcta cagcatgacc
     1081 agtgccggtg tcgtcaacat gttcccgcac accgggcacg ttgaatccat cgcggtgttc
     1141 gacgccacgc cgcaagccgc ctgacagccg tggcgtttga tactctgata ccccggcccg
     1201 cgcgctacgc ctggttggcc gactcggcca gaatccgttc ggcttccagc ttgacccgtt
     1261 caggtgccgt gccgccaatg tgcttgcgcg acgccacgga accttcgagt gtgagcacct
     1321 cgtagacatc ggcgtcgatg gagggatgga aggcacgcag ctgttccaaa ggaaggtcag
     1381 ccagatcgca tccgcgttct tcgcaggtcc gcacggccag cgccacggtt tcgtgcgcgt
     1441 cgcggaacgg tgtgcctttc ttgaccagat agtcggccag gtcggtggcg gtcgcgaagc
     1501 cttgcagggc ggccgcgcgc atggcctcgg ccttgacgcg gatgccgccc accatgtcgg
     1561 cgaagatggt cagtgtgtcg cgtatggtgt cggcggtgtc gaacaagcct tccttgtcct
     1621 cctggttgtc cttgttgtag gccaggggct ggcctttcat cagggtcagc agggccatca
     1681 agtggccatt gacccggccg gtcttgccgc gtgcgagctc gggcacgtcg ggattcttct
     1741 tctggggcat gatggaactg cccgtgcaga agcggtcggc cagttcgatg aagcccacgc
     1801 gcgggctgac ccagagcacc agttcttcgg ataagcgcga cacatgcacc atgatcagcg
     1861 ccgcgtcggc gcagaattcg atggcgaaat cgcggtcgga gacagcgtcg agcgagttcc
     1921 ggcagacgcc atcgaaaccc agggttcgcg cgacgcgctc acggtcaatg ggataggagg
     1981 tgccggccag cgcagcggcg cccagcggca gtcgattgac gcggtggcgg cagtcggcca
     2041 ggcgctcgat gtcgcgcccg aacatttcgg cgtaagccag cagatggtgg ccgaaagtga
     2101 ccggctgcgc cacctgcagg tgcgtgaagc ccggcaggat ggtggcggcg tgctccagcg
     2161 ccaaagtggc gaggttgcga cgcagctggc gcagcaggcc cacacaggtg tcgatctcgg
     2221 cacggaccca caggcggatg tcggtggcca cctggtcgtt gcgcgagcgg cccgtgtgca
     2281 accgcttgcc cgcgtcgccg acgagctcga ccagacgttt ctcgatgttc agatggacgt
     2341 cttccaggtc gagcgaccaa cggaagtccc cactgtcgat ctcttgacga atgcgggcca
     2401 tgccgcgctg gatggcggtg agatcgtccg cgctgatgac gccgacggcg gccagcatgt
     2461 cggcgtgcgc cagcgacccg tcgatgtcgc aatgcgccat gcgttggtcg aatcccactg
     2521 acgcggtgta gcgtttgacc agatcggaga ccggttcgga gaagcgcgct gaccaggcct
     2581 tggccttgtt ggcgaactgg tcgtgggaag ggtcggaaga aggggtgggt gtgttgtttg
     2641 gcatggtggc ggaattataa tggacgcgtc ggccgtcaag acatccgcat ggctgggccg
     2701 cgctccgcac gcgtgcgata atttccctct ttcttttctc gttcaaggct ttgcgacatg
     2761 acgacagtgc ggatcggtct ggcccagatc aacaccgccg tgggcaacct gccagccaat
     2821 gccgcccgca tcctcgacgc ggcgcgctgg gcgtccgaac atcgggccga cgcgctgttg
     2881 acccctgagc tggggttgac cggctacccc gcagaagacc tgctgatacg gcccgcgttc
     2941 atcgaccggc agcttgacgt gctggatcgc ctgcgtggtg atctggcgcg gtttcgcggt
     3001 ctgcatgtca tcgtcgggca tgtgcggcgg gatggcaacc gtctctacaa ctccgccagc
     3061 gtcatggccg atgggcgtgt cgtggccacc tacgacaagc acgaattgcc caactacgcc
     3121 gtattcgacg aaaagcgcta cttcgcgccg ggcgccaagc ccgccgtgtt cgatctgcgc
     3181 gggctgaaat gcggcgtggg tatttgcgaa gacacctggt tccccgacag ccccgccaag
     3241 gccgcggcgc gggatgccaa ggtttggctg gtgctcaatg cctcgccttt cacgcttgcc
     3301 aagcaggacg agcgtatccg ccaggccgcc acgaatgcgc aaggcatggc cacggtctat
     3361 ctcaacaaag tgggcgggca ggacgaactg gtgttcgatg gcgcctcgtt cgtgctggac
     3421 gcacggggcc gggtggtgaa gcgcctgccg gtgtttgaag agtgcctgga cctggtcgaa
     3481 ttcgactcgc ttggcgagcc cgccctggcg gagtccccgg cgcaatggcc ggatgagccc
     3541 gagcaggtgt ggcgggcgct ggtgctggcg gtgcgcgatt atctggccaa gacgggcttt
     3601 tcaaaggtcg tgctgggtct gtcgggaggc atcgattcgg ccgtcgtcct ggctgtcgca
     3661 gtcgatgcgc tcggcgccga caaggtccgt gccgtgatga tgccgtcgcg atacaccgcc
     3721 gacatgtcgt tggccgatgc acgccagatg gccgacacgc tgggcgtcgc ctatgacgaa
     3781 atcggcatat ccagcctggc cacggcattc gaccaagcat tggcgccgca attcgccggg
     3841 ttgccgttgg atgccacgga agaaaacatc caggcccgca tacgtggcat tttgctcatg
     3901 gcgttgtcca acaagaccgg cgccctggtg ctttccacgg gcaataaatc agagctcgcc
     3961 acgggctatt gcacgctgta tggcgacatg gcgggtgggt tcgcggtgat caaggatgtg
     4021 ccgaagacgc tggtctatcg gttggcccgt tggcgcaacg gtgtatcgtc tgtgataccc
     4081 cagcgcatca tcgaccgggc gccttcggcc gagttgcgtc cggaccagac cgaccaggac
     4141 agcctgccac cctacgacgt gctcgacgcc atcgtcgagc gctatgtgga gcataacgag
     4201 cccagggacg acatcgtgcg atcggggttt gacgccgacg tggtggatca gatcctgcga
     4261 ttgatccgca tcaatgaata taaacgccgc cagggggcgc cggggcccaa gatcaccacg
     4321 cgcgcattcg gccgcgactg gcggtttccc atcgccaacg ggtttcgcga atgacccggt
     4381 cgcgggcatc cgccttaccg ttcagagccg gacaaccggc gcactcacgc cagaagggag
     4441 actcatcatc atgaagcagg tcacggccat catcaaaccg ttcaagctcg acgaagtgcg
     4501 tgaagccctg gccgaaatcg gtgtcagcgg tctgaccgtc accgaggtca aggggttcgg
     4561 gcgccagaaa ggccacaccg aactataccg gggcgcggaa tacgtagtcg atttcctgcc
     4621 caagatccgc atcgacgtcg cgctcgcgga cgatatggtc gaatcggcca tcgatgccat
     4681 tgtcaaggcc gcccgcaccg gcaagatcgg cgatggcaag attttcgtca cgccagtcga
     4741 gcaggcgatt cgcatacgca ccggcgagat cggggccgaa gcgttgtaga atgccgtccg
     4801 ccacgatttg gcgcgtgtcg tcgcccggcc gggatgccgc atcgcgggcg cgttggatgg
     4861 cattctgcga acaacactga atttgcccgg atattcgcgg tattagggta tttcttgaaa
     4921 gaattagggt atttcttgaa ggaaattcag tgattattca ggcatactgg gcgtctggtt
     4981 tggtggcgtt gattcgtcac agatgctttg gatggttggc gccctagcgc caaccatgac
     5041 aaactgtata aacttgcgaa cgtttgcttc gttcaatcgc cgcaaacgat tcatcgctca
     5101 acatataaaa caggttttca cacaggagaa gctatgaaac tattgccgct cgtactggcg
     5161 acagctgtca ccgcggtggg gatgaccgcg gcgcaggctg cggacagccg gatcgacaag
     5221 atcaaatcga cgggggagat cacgctgggg tatcgtgaat cgtcgatccc gttctcgtac
     5281 ctggacgaca accagaagcc catcggctac tcgatggaca tctgctatgg cgtggtcgat
     5341 gcgctcaaga aagacctgaa gatgccgaac atcaaggtca agctggtgtc ggtgacgtcg
     5401 tccacgcgta ttccgctggt ggccaacggc acgatcgacc tgtcatgcgg gtcggccacc
     5461 aacaacgcgc aacgccaggt gcaggtcagc ttcgcgccta ccacgttcgt gacagccacg
     5521 cgtttcaccg cgctcaagaa gtcgcacgtc aacgacctga aggacttcaa gggcaagacg
     5581 gtggtgtcca cctcgggcac cacgaacctg aaatggctca cccagaccaa cgccgacgag
     5641 cacctgggca tgcgcatcat cccggccaag gaccactccg aagcgttcct cacggtggcc
     5701 agcggacgcg cctcggcgtt cttcatggac gacatcctgt tggcgggcct ggtggccaac
     5761 tcgcgcaatc cgggcgagtg gatgatcagc aagcacgcct acacgatcga gccctacggc
     5821 atcatcgaac ccaagggtga cccgcagttc aagaccgctg tcgatgacgc ggtcaaggcc
     5881 atgatgaaag acggcaagct cgatcagctc tatgccaaat ggttcacgaa gccgatcccg
     5941 ccgaagaaca tcaacctgaa ctggccgatg acgccggaac tgaagaaggc attcgctcac
     6001 ccgacggatt cgcccgaccc tgcggtctac aaataagccg cgctcgtagc tgacgcagct
     6061 tcatttggtt tgtactggcg gcacaacccg gtatcttcaa gggttgtgcc gtttctgtaa
     6121 gagggtgtgg gcctacaccg ttaaggacag ctttaatgaa ttaccactgg aactggatga
     6181 tcttctgggc gcaggcaccc gacgggaccg gcacctacat gcatacactc atccgcggca
     6241 tgtactggac gctcggcgtg tccattcttg cgtggatcat cgccctggtc ttcggggtga
     6301 tggtcggggt gttccgcacc acccgcatcg gctggctgga acgcctctgc gcgggctacg
     6361 tcgagctctt ccgcaacgtg ccactgctcg tgcagatgtt cctgtggtat ttcgtgtttc
     6421 ccgaggtcgt gcccagcgcg atcggcaact ggatcaaggc ggccgatccg acctggagca
     6481 cgttctggac ggcggtggta tgcctggggt tgttcacctc ggcgcgtatc gccgagcagg
     6541 tgcgcgccgg cgtcacgtcg ttgccgcgcg ggcagaacat ggcgggcacg gcgctggggc
     6601 tcacgcgccc acaggtctac cgttacctgc tgctgcccat gacggtgcgc atcatcatgc
     6661 cgccgctcac ctccgaggcg ttgaacctca tcaagaactc gtcggtggct ttcaccatcg
     6721 gcctgctcga gatcacgggg gccgcgcgct cgatgcagga attctcgttc cagatcttcg
     6781 agtcgttcac ggccgccaca gttctgtacc tgatcatcaa cgcaatcgtg gtggtgctca
     6841 tgcgctacat cgagaagtgg atcagcgtac cgggcttcat cggtacaaag agataagggg
     6901 cgcatcatgc acggattcga ttttgaagtc atctggcgca cgctgcccta tgtgttcggc
     6961 acggggatgg tgttcacgct gaaactgacc atactgtcca ccatcggagc gcttatcctg
     7021 ggcacgttgc tggcgatgat gcgcctctcc tcgaaccgct tcatcgcggg ggtgtcgggc
     7081 gggtacgtga atttcctgcg cgcgctgcca ctcatcctga tcattttctg gttctatttc
     7141 ctggtgccgt acatcttcgg gtggatcacg cgggcagggc accccatatc cgtcgggggg
     7201 ttcacctcgg cgttgatcac gttcgtcctg ttcgaggcgg cgtacttttg cgagatcatg
     7261 cgcgcgggca tccaatccat tccacgcggg cagatcggcg caggctacgc gctgggcctg
     7321 aactactggc aggtgatggg acacatcgtg ttgccccagg cgtttcgcaa catgacgccg
     7381 gtgatcctca cccagatgat cgtcctgttc caggacacct cgctggtgta cgtgctctcg
     7441 ctcaccgact ttcttggcgc ggccaacaac gtggcgcagc gcgacggccg tctggtcgag
     7501 atgttcatct tcgccgccgt ggtctatttc cttatttcct atgcggcgtc acgctcggtc
     7561 aagcacctcg agcgtcgctt cgccattgtt cgttaatgcc cggagtcatc aatgattgaa
     7621 ttcaagaacg tcagcaagtg gtatggagcc tttcaggtgt tgaccgactg ttccacatct
     7681 gtgtccaagg gagaggtcgt ggtggtgtgc ggcccatcgg gctcgggcaa atccacgttg
     7741 atcaagacag ccaacgcgct ggaaccgttc cagaaaggcg agatcttcat cgatggggtc
     7801 tctgtcggcg catcttccac caacctgccc aagctgcgct cgcgcatcgg catggtgttc
     7861 cagcacttcg aactgttccc gcacttgtcg gtcctgcgca acctgtcgct ggcgcagatc
     7921 aaggtgctca agcgccccga ggccgaggcg gtgaagcgcg cccgcgcgct gcttgaacgc
     7981 gtgggcctga gcgaccatat caacaagtat cccgggcaat tgtcgggtgg ccagcagcag
     8041 cgcgtggcca tcgcccgggc actggccatg gaccccatcg ccatgttgtt cgacgaaccc
     8101 acctcggcgc tagacccgga aatggtcaac gaggtgctcg acgtgatggt ggacctggcc
     8161 aaggagggca tgaccatgat ggtggtcacg cacgagatgg gcttcgccaa gaaggtggcc
     8221 gaccgcgtca tcttcatgga cgcaggcaag atcgtggagg actgctccaa agacgacttc
     8281 ttcgaccatc ccgaccaacg ctcggatcgg gccaagcagt tcctgtcgaa gattctgtcg
     8341 cattgagacc gatcggcgcg agggcgggct gccaccgatc tttccgggaa agcccgccgc
     8401 cgagaatggg gattgctgca gtgcctcgcg ggttcgtgct acgctcctgc ggatggcact
     8461 attgatcaca tcatgtcgga taccgatcct ccgttgaccg atttcgcttc gctgctggtg
     8521 ctggcgtata agcaggaaga gccgcagcgc atgttgttcg tgtttgcccg caaagaactt
     8581 cccgagcacg cctctattgg gcagcgggag cgtttcaaca acggagaggg tggtggcctt
     8641 cgcccatgcc tgtgtgtgga caaggcgctg gaagaaatct ccagcttcga atcgctggtg
     8701 gctgaatcgg ctgccacggg ccagtcttgg gatgtggcgt tcgtgggttg cctcgccggg
     8761 cgtggtggca tcccgcccaa cccggacgaa gcggattatc ctctgcgcct gatggtcaac
     8821 gcgatcaaca acggccgtgt cagcgacctt gctgcgttcg accggaacgg gcgcgcattg
     8881 cgattcattt aggcctgcct gccgtcagcg gccagaatcc gggcaggaaa cattcagcca
     8941 ccagcagcgg gtatccgtgg cgccaaaaca ccgagcagcg ggcgaaaagc tcgtgcgcgg
     9001 tgggcgtcgg tgtaaccagg gcacgcttga cggctccgta caggggttgg ctggggcgca
     9061 accgacacac gaagaaacgt gaccgggtga tttgggggtt gtgatagagc atgtcggcca
     9121 gtgggcggcg gttaagcgca cgcatgcctc tccaccacga aaatgacgca ttccgcggcg
     9181 tgaagctgcg cgccacgacg ctatcgacac cattgatcgc catcttgatt tcgcggatgt
     9241 gcatggcgga tccgggcggg cgtctgacca tccacgcttc gcgggcggac aggccatcgg
     9301 tgtattcgct gacgaccgtc aagtccacat ggccgagctg ccgcaggccc gcagtgaggg
     9361 cgccagggcg cagcagccag tgtttttgtt cgtgggtgag ggaagggggg gcgagttcgt
     9421 gccagttgcc gggattgatt gcttcgggat ccatagcctg tattatcgcc aaactcctac
     9481 aatgtcggct atggaaaaag tacggatttc aaaactgatg gccgagcgtg gcttgtgttc
     9541 acgacgcgag gcggatgcct atattgaaaa aggctgggtc cgggtcgatg gcatcgtggt
     9601 gtccgaactg ggcagcaagg cgttgcccag ccagaccatc acgctggaac gccaggcgcg
     9661 tgaaaaacag gtggcccgtg tgaccattct gttgaacaag ccagtgggtt atgtgtcggc
     9721 gcaggccgag aaaggctatc ggcccgcagt atccctcatc gatgccgcat cacagtacca
     9781 cgccgacgcc tcccgcttgc ggttcgaccg cagtcacctc cgtgggctcg ccccggccgg
     9841 acggctggac atcgactccc aggggctgct ggtgctgacc caggacggtc gcatcgcgcg
     9901 ccaactgatt ggcgatgact ccagtgtgga caaggagtat ctggtgcggg tgcaggggcg
     9961 tctcgccgcc agtggcctgg cattgctcaa tcatggattg acgctggatg gccgaccgtt
    10021 gaagccggct caagtcacct ggcagaacga cgaccagttg cgcttcatcc tacgcgaggg
    10081 caagaaacgt cagattcgcc gcatgtgtga actggtgggt ctgcgcgtgg tgggactcaa
    10141 gcgggtgcgc atggggcggg tggtgttggg cgatctgccc gaggggaaat ggcgctacct
    10201 gaagccaggg gagcggttca tctagagtca cgcaccacaa gtcgccgctg gcgattctcg
    10261 actgctttat gtcgactgct tgatgaaacg aacttgtggg acatgacgct agagccgtgg
    10321 atcgcttcca ccgtcccgac gtcaagaggt cttttggttc agcgtgaatc gcgcgcggtc
    10381 atcctggccc agccgctggc gcaaccaggg tagcgcctgt tccagcgcgg cgtgcagtgt
    10441 ccacggcgga ttgaccacga agacgccgct accgtgcagg cccaatccgt cggacgccgg
    10501 tttgcgcacc gagaggctcg cgtgcagcca gctctttgcc ggcaggcgtt ccaacgagcg
    10561 ggccagatcc agcgactcga cccgctgcac caacggatac cagaccatga agcagcccgt
    10621 ggcgaagcgc ttgagcccat cgttcacggc attcagcgtg cgccggtagt ccgctttgtt
    10681 ttcgtaagag gggtcaatga tgacaacccc gcgtcgggtc aagggaggca gcaaggcttt
    10741 cagcccgtcg aatccgtcgg catcgtggat ttgcgtctgt ttgcgggcca actgccccat
    10801 ggccgccaga tttttcctca ggacatcgac ttccgtcggg tgtttctcga ataggtgcag
    10861 gcggtcctgt tcgcgcaggg cccgcaatgc cagccaagga gaccctgggt agaaattggc
    10921 aacgccatcg gggttgtggt cctggacggc tcgcaggtag ttctcgacca gtgctggcat
    10981 atccggcgcg cccagcaatc gatccaatcc atcgacatat tcggcgttct gtgccgccca
    11041 gtcgtccgcc aggtcgtaga tgcccgcccc ggcatgcgtg tccacgaccc aatagggtga
    11101 ttccttctgg ttgaagtagt cgaggatgtg gacgaatatc gcgtgcttga ggacgtccgc
    11161 gtggttgccg gcgtggaacg cgtgtcggta gctgaacaat gggggccttt ggtcgcggct
    11221 tagatggaag gcggaatggc ttcgatctcg tcggtgaaga tggcgtcgca tccccagtcg
    11281 agcaggtcgc gggcgcgcac ggtgtcgttg accgtccagg ctgtgatctt gcagcctgtc
    11341 tcgtggacat ggttgatgat gtccggcgtg acgaagcgct ggttcatgtt caacgccacg
    11401 catcccaggc ggcgcaggcg gtcgcgccag tcgggtggaa gctcgccctc gatcagcagg
    11461 gcgcgtggca attcgggtgc ttcacgctgg gccgccgcca gggcgatttc ggaaaaagac
    11521 gacatcaggg gcggtacctg ggcatgcacc cacatcaggc gggccatgcg cgcgaccaat
    11581 gtgccagtgt gatcgtcggt gccggtgtgc ggcttgattt ctatgttgct gagaacgttg
    11641 ttgtgttggg tgaaagccgc gatggcgtgg agcgtgggga tgggctcgcc ggcgtagcgc
    11701 accgagtgcc aggcgccgaa atcgagcgtg gccagttcac ggtaagtcat ggcgccagcg
    11761 tcgccgctgc cgttcgacgt gcggtcgatc ttgtcgtcat gaagcaacac ggcgacatcg
    11821 tcgctggtga gcttgacgtc gtactcgacc atggtgaagc cgcgctgggc gcccaggcgc
    11881 atggaggcga gtgtgttttc aggcgcgaag tgcccacctc cccggtgtgc gatgacgcgt
    11941 gggtagggcc agatgaatga ggtatcggac attttctgtc tttacaccga atgaggatga
    12001 tccagacact attctatagg ttaagcttgc ggcctgcttc cggggttgcg cccggttccg
    12061 tgccttgaaa atggtaaact cccagggttt tggggtatgc cgcttccggg cgggtggcca
    12121 tttagcgagt ccgttccatg ttcgacttca tccgttcgca ccaacgcctc atgcaggtga
    12181 tcctgctggt gttgattctc ccctcgtttg cgctcattgg cgttagcggg tacagcacgt
    12241 atgtgtcggg cgaccatgac atggtcaagg tcggcgggca tgcgatcacg ctccaggatt
    12301 acgaccaggc ccgacgcaat cagttgcggg agctgcagtc tcagaatcgg gccggattcg
    12361 acccgtcttc gctcgataat gaggccgccc gcagtgcgct gctcgaatcc atgatcgatc
    12421 gccgcgtcat cgccaccgtg gccagcgacg accgtttcag cgtatccgat gcggttctgc
    12481 gccaagccat cgcggccatt ccgcagcttc aggtcaatgg tgaattttcc tccgagcgtt
    12541 accgtgatct gctcaagtcc atgggcatga ccagccgcga tttcgaacgc ggtcagcgcg
    12601 ccgaactcgc gctgggccgg gtgctcggcc cggtcgatac ctccgccaca ttgccgcggc
    12661 ccgtcactga ttccatcgaa cgcgcgctga ccgctcagcg caccgtgcgc ctgcgcatct
    12721 atcctgtgtc caactacgag aaggatgtga aggtttccga tgcggacatc aaatcctggt
    12781 acgacggcaa caagcagcag ctcaagctgc ccgagcaagt cgatatccaa tatttgctgc
    12841 tcgatgaagc cgcagccatg aagaacgttc cggccgtcag cccggatgcc gaaaagaaat
    12901 actacgaaca gaacaaatca cgcttcgtca gcccggcgcg agtgaatctc agtcatatcc
    12961 tggtttcggt gcccgttggg gccacggaag cgcaacgcga tgcagcgttg aagaaagcgc
    13021 gcaagatcga tacggaagtc caggcgagca aatcgcaatt cgccgatatc gcacgcaagc
    13081 aatcccagga cgccggcacg gcgcgccagg gtggtgaact gggctggatc acacagggtt
    13141 catggcccgc caatctcgac aaggtggtgt tcgcgttgaa gaaaggcgag gtctccggcg
    13201 ttgtcgatgg ccctggcggc taccatatct tcaaggccaa cgacgtcgat ccttccaaga
    13261 cgcagtcttt cgaagacgcg aaaccccagg tcgaaaagga aattcgccag caactggccg
    13321 ccgacaaatt cgccgacatg tccaccaggc tcaccagtct ggtctatgac aatgccagca
    13381 gcctgcaacc ggcggctgat gcgctggggt tgcaggtcaa gacggccagc ggcattgccc
    13441 gcgagcgcct gctcgacgcc aatgaggcgg gctccggcgc cgccgccacc agcccagatg
    13501 cggcagtgct ggccgatgta cgcgtgcgcc gggcgttgtt cacgccccgg gtgctcactg
    13561 acaagcagaa ttcaggcgtg atcgaaatct cccccgatac actgatcgtc gtccgggttg
    13621 ccaagtccat cccggcgcat attccaccac tggacaaggt caccgccacc atccgcaaga
    13681 cattgatcga caagaaagcc ctggcggatg cggtcaaggc tggtcaggcc gccgtggcag
    13741 ggtaccgcaa ggcaggcgat tcggccaagg tgcctgacgg tttcggttcg gccatggcgg
    13801 tcagccgtat cgacccgcaa gggctcagca agcccgttct ggatgcggcc ttggcggcgt
    13861 ccgtcaagac tttaccaacc tatgcgggca tgcaggcccc cactgcgtat gtggtcatac
    13921 gcatagagaa ggccgagccg ggcaaggcgg gcgacacaga gctggcgggt ttgccggctc
    13981 aactcgacca gctttggggc aatgcggaaa aagccgcggt gctcaaggcg ctgcgccagg
    14041 aagtgaaggt caagatgctg ccggaatcca agaaggtgtt gtctggcgag accgagaacc
    14101 aggatagcta gcgcggtttt ggtttgagcg cttttggctt ttgtttagat gcttacgagt
    14161 tgagtgatgt tcttggtatc tgaaggcgct gtctatcggg gcttgggcgg gctcggcacg
    14221 gcgtgcttca tccggatctg gcttgaccag gcgggggcag tcgtcgggcg gtgtttgagc
    14281 gaggcctcgc aatagcgagg cgtagcgagt ttagcccgac gcccgcctgg ccaagccaga
    14341 cccgggcagt cggggcgcgc agcgccacga ccgctggacg cgccggcttg caccacctcc
    14401 cccaccctgg ggcgggttaa tagaatagaa cccaccccga caaaaacggc caccccgacc
    14461 acaacggtca aaacggttca atcggttgaa gcagcaacgc aatccatgct cagcgttgcc
    14521 tacacttcaa ccgaaaaggc tcaacgttgt tggctggcct cttgctgggc atcgaccacc
    14581 gccagcgccg tcatgttgac gatgcggcgc accgtggcgc tggtcgtcaa cacgtgcacg
    14641 ggccgtgccg cacccaacaa cacgggcccc atggccaccc cgtggctgcc cgtcatcttc
    14701 agcatgttgt aggtgatgtt gccagtgtcc aggttgggcg tgatcaacaa attcgccggc
    14761 cccttcaggg aagaatcggg ataggccttc aggcgtatgg actcggacag cgccgaatcc
    14821 gcatgcattt cgccatcgac ctcgagatcg ggagcgcgtt ccgcgatgat ctgacgtgcc
    14881 cgcgccatct tgcgcgaaga ctctgtgggg cggctgccaa agttcgaatg agacagcaac
    14941 gccaccttgg gcgtgacgcc gaaacgcaag acctcgcggg ccgcctggat cgtcatgtcg
    15001 gcgatttgtt cggcatcggg attctcgttc acgtgcgtgt cgcagatgaa cagggtctgt
    15061 gtcggcagca tcaacacgtt catggccgcg taggtgcggg cacccggttt caggccaatg
    15121 acttcctgaa catacttgag ctggttgtca taccggctgg cggcgccgca caacaaggca
    15181 tcggcgtcgc cacgttgcag cagcatgacg ccgatgagcg aattgtgttt gcgcaccatg
    15241 gccttggcga tttcgggtgt gacgccttcc cggccctgca gcttgtagta tgccgtccag
    15301 gtgtcatgga aacggctgtc gtcttcgggg ttgacgatct cgagatccga agcgaggtca
    15361 agtcgaagcc cgaacttgcg cacacgcatt tcgatgacgg aagggcgccc gatgagaatg
    15421 ggccgggcga tgcgttcatc gaccacggtt tgcaccgcgc gcaggacgcg ttcgtcctcg
    15481 ccgtcggcgt agatcacacg cttgggatcg gccttggcct gcatgaagag tgggcgcatg
    15541 agctggccgg tgtggtagac gatgctcatc agccgctggc gataggcctc catgtcttcg
    15601 atggggcgcg tggcgacgcc ggaatccatg gccgcttgcg ccacggccgg cgcgatcttg
    15661 atgatgagtc gcgaatcgaa cggcttgggg atgatgtaat ccggcccgaa caccagttcc
    15721 tggccgacat aggcgctggc cacctcatcg ttctgctcgg cttcggccag cgaggcgatg
    15781 gccttgacgc aggccagttt catttcctcg ttgatgacgg tggcgcccac gtccatggcg
    15841 ccgcggaaaa tgaatggaaa gcacaggacg ttgttgacct ggttgggata gtcagatcgg
    15901 cccgtggcga cgatgcagtc ggagcgggcg gccttggcgg cttccggccg gatttccggt
    15961 tcggggttgg ccaaggccag gatgaggggg cggggtgcca tggtcttcac catgtccggg
    16021 gtcagcacgc cgggcgccga gcaacccagg aacacatcgg catcgcggca gacgtcggcc
    16081 agagtgcgcg cgtcggtatc ctgggcgtaa cgcgccttgt tgggctccat gttctggtca
    16141 cgccccttcc agatgacgcc gcgcgaatcc accacgaaga tgttggcgcg ggccaccccc
    16201 aggtgcacca gcagatcgag gcaggcgatc gaggccgcgc ccgcgcccga gcacaccagt
    16261 ttgacgtcgc cgatgttctt gtcgacaacc ttcaacccgt tgaggatggc ggccgacgag
    16321 atgatcgccg tgccgtgctg atcgtcgtgg aacaccggaa tcttcaggcg ttcgcgcagc
    16381 ttcttttcga tgtagaagca ttcgggagcc ttgatgtctt caagattgat gccgcccagc
    16441 gtgggctcta gcgcggcgat gacgtcgacc agtttgtcgg gatcgttttc gttgagttcg
    16501 atatcgaaga catcgatgcc ggcgaacttc ttgaagagac agccttttcc ttccatgacc
    16561 ggtttcgccg ccagcggacc gatgttgccc agccccagca ccgcggttcc gttggtgatg
    16621 acgcccacca ggttcgaacg cgacgtgtat ttcgcggcgg cttcagggcc atcggcatga
    16681 atggccatgc acgcgtaggc cacgccaggc gagtatgcca gcgacagatc gtcctggttg
    16741 gccagcggct tggtgggtgt gatggatatc ttgcccgggg tggggtaggc gtggtagtcg
    16801 agagccagtt tggcgaggtc ggctttggca tccatgatgg aagggctcct gtgatgtcga
    16861 aaattcggga ggcgcgggcg ggcggcgcgc gcacggttaa tcataggctt gttcgaacca
    16921 tggcgggagg cattgtctcc attcatggcg gaaggccggc cgccgggcag cgggcccggg
    16981 aaaaaggcgt aagtgtacgt ctgttcgcgc gccgtgttcg acgggggcac gtgttccaag
    17041 tgtttgttaa tgccaacgcc cctgcactat ttggcccggc gcgcgccatt tcccgtgcct
    17101 tgagtggggg atcgagggta tactaccgtc caatacggct tatcaaggcg cccgtacgtt
    17161 gccttctgcc ccgctatccg ctatccggtc tggccgtgtc gcggaaggtt gtcctgcatc
    17221 ataacgagtc agagactcgg ataggtgaag cgaatatatg tcatcggtat cagaactcaa
    17281 gtcaaactcc tacctgtttg gcagcaatgc cccttatgtc gaggaactct acgagtcttt
    17341 cctcgatgat cctggttcgg tgcccgacaa gtggcgcgac tacttcgacc agcttcaaca
    17401 cactcccgcc accgatggca gcgagatcac acgggatcag gcccacgccc ccatcattga
    17461 atcgttcgcg cagcgcgcca agagcaatgg tttcgccgtg cgcgtcaaag cgcccgatct
    17521 ggccgtggcc ggcaagcagg tgtatgtgca gtccatcatc gcggcctacc gttcgctggg
    17581 ttcacgcttc gcccagctcg atccgctcaa gcgccacgat cgccccaaca tcccggagct
    17641 cgaccccggt ttttatggcc tgaccgaagc cgacctggat caggtctatt cggccaccaa
    17701 cacgtatttc accaaagccg acacaatgac aatgcgcgac atgctcaagg cattgcgcga
    17761 tacatactgt cgcgacgtcg gcgccgagtt catgcatatt tccgatccgg cttgcaagcg
    17821 ctggattcag gagcgcctgg aatccaccat gggtgtgccg gcgtattcgg cggagcgcaa
    17881 gcgccacatc ctgcagcaag tcaccgaggc cgagggtctc gagcgttttc tgcataccaa
    17941 atacgtgggc cagaaacgtt tctcgctcga aggcggggaa agcttcatcg cctccatgga
    18001 tgaagtcgtc aaccatgcgg gtgacaacgg cgtccaggaa ctcgtcgtgg gcatggccca
    18061 ccgtggccgc ctgaacatgc tggtcaacat catgggcaag atgccgggtg acttgttcgc
    18121 cgagttcgag ggcaagcacg ccgagggcct gtccgatggc gacgtcaagt atcacaacgg
    18181 tttttccagt gatctgtcca cacgcggcgg ccccatccat ctttccctgg cgttcaaccc
    18241 gtcgcacctg gaaatcgtca atcccgtggt tgaaggcagc gtgcgcgcgc gccaggatcg
    18301 ccgcgccgat gtccatggct cggaagtgtt gccggttctg gtgcatggcg atgccgcgtt
    18361 cgcgggccag ggcgtggtca tggaaaccct gaatctggcg caaacccgtg gctacggtac
    18421 cggtggcacg ctgcatatcg tcatcaacaa ccagatcggg ttcaccacct ccgacccgcg
    18481 tgatacacgt tccaccctgt attgcaccga cgtggtcaaa atgatcgagg cgccggtgtt
    18541 ccacgtgaac ggtgacgatc cggaagccgt ggtctacgtg accagtctgg cgctcgacta
    18601 ccgccgcgaa ttccaccatg acgtcgtggt cgacatcgtc tgcttccgca aactgggcca
    18661 caacgaacaa gacacgccgt cgctcaccca gccgctcatg tacaagagca tcggcaagca
    18721 cccgggcacc cgcaaggtct acgccgacaa gctggtggct cagggcgtgc tggccgaagg
    18781 tgaacccgac cagatggtga aggattaccg ccagatcatg gaagacgggc ggcgcaccgt
    18841 cgagcctgtg ctgaccgact acaagaataa gtacgccacc gactggtcgg cgtttctcaa
    18901 cgccaaatgg accgatcagg ccgatacggg tgtgccgctg agcgaactga cccgcatcgg
    18961 tgagcgcatc accacgattc cagacggctt cacgctgcac ccgctggtca gtaaattgct
    19021 caatgatcgc cgcaacatgg tgcgcggcga gcacaagctc gattggggca tgggtgagca
    19081 tctcgcattc gcgacgctgt tgtcttccgg ttatgccatt cgcatcaccg gccaggattc
    19141 gggccgcggc acattctccc accgccatgc cgtcctgcac gatcagaagc gggagcgctg
    19201 ggacgacggc acctacattc cgttgcagaa cgtgtccgac acccaggcgt ccttcacggt
    19261 catcgactcg gtgttgtccg aagaagccgt gctggcgttc gagtatggct atgccaccgc
    19321 cgaacccaac gtgctgacca tatgggaagc gcagtttggc gatttcgtca acggcgcgca
    19381 ggtcgtgatc gatcagttca tcacctcggg cgaagccaag tggggccgcc agtgcggcct
    19441 gaccctgatg ctgccgcacg gctacgaagg gcaaggtccg gagcactcgt cggcgcgtgt
    19501 cgaacgtttc ctgcaattgt gcgccgacaa caacatccag gcggtccagc ccaccaacgg
    19561 cgcgcagata ttccacgtgc tgcgtcgcca gatgatccgg ccgttccgca agccgctggt
    19621 gatcttcacg cccaaatcgc tgctgcgcaa caaagatgcc tcgtcggatc tcgacgatct
    19681 cgtcaacggc cggttcctgc ccgtcattgg cgagcttgac gccgccatcg accccgatgg
    19741 ggtcaagcgc gttttggtgt gctcgggcaa ggtgtactac gatatcgtgc atgcgcgggc
    19801 cgccgcgggc cgcaccgatg tggccgtgct gcgcgtcgaa cagctttatc cgttcgcgca
    19861 caaggcgttc cagaccgagc tgctgaaata caagaacgcc cgggaactgg tctgggtgca
    19921 ggatgaaccc cagaatcagg gtgcctggtt ctatgtgcag catcacctat acgagaacat
    19981 ggctgagggc cagaaactgg gctatgcggg ccgcccggca tcagcctcgc cggcggtcgg
    20041 ctacctggcc aagcaccagg aacagcaaaa gacgcttctc gagcaggcgc tggcgcccaa
    20101 gttcaagggc tacatgctcg ccaaataatc ccattattca aagacggaat ccacatacca
    20161 tggccatcac tgaagtcgtc gtcccccagc tttccgagtc ggtctccgaa gccacgctcc
    20221 tcaattggaa gaaacagcct ggcgaagcca tcgaagccga tgaaatcctg atcgaagtcg
    20281 aaaccgacaa ggtcgttctc gaagtccccg cgcccgtgtc gggcgtgctt tcggaaatcg
    20341 tcaagggcga tggcagcacg gtgacctcgg gcgaggtgct ggcccgcatc gataccgaag
    20401 ccaaggcagc ggccccggcg gccgcccccg ccaaggccgc accggcggca ccggcgccag
    20461 ccgcagccac tgccgcgcct gtggcgtcag ccgcagcttc gcctgccgcc gccaagatcc
    20521 tggccgaaaa aggggttgac ccggcttcgg tggaaggctc gggccggggt gggcgcatca
    20581 ccaagggtga cgcgttgcag gccggtggcc agcccgcgcc caaggcagcc gcacccgtgg
    20641 caccgcccac gttgtcgctc gacggtcgcc cggaacagcg cgtgcccatg agccgcctgc
    20701 gcgcgcgcgt tgctgaacgc ctgctgcaat cacaatccga gaacgccatt ctcacgacgt
    20761 tcaacgaagt cagcatgcag gccattctcg atctgcgcag ccgctacaaa gaaaaatttg
    20821 aaaaagagca cggtatcaag cttggcttca cgtcgttctt cgtcaaggcg gccgtggcgg
    20881 cgctcaagaa gtaccctgtc gtcaacgcgt cggtggatgg caaggacatc atctatcacg
    20941 gctatttcga catcggtgtc gcggttggca gcccgcgcgg cctggtggtg cccattgtgc
    21001 gcaacgccga tcaactctcc atcgccgaca tcgagaagca gatcgccgat ttcggtgcgc
    21061 gtgcgcgtga cggcaagctc gccatcgaag agctcaccgg tggcacattc tcgatctcca
    21121 acggcggcgt attcggctcc atgctttcca cgcccatcat caacccgccg caggcggcca
    21181 ttcttggcat tcacgccacg aaagaccgtg cggtggtcga aaacggccag atcgtgatcc
    21241 ggcccatgaa ctacctggcc ctgtcctatg atcatcgcat cattgacggg cgcgaggcgg
    21301 tgctggcgtt ggtggccatg aaagaagcgc tggaagatcc gcagcgccta ctgctcgaac
    21361 tgtaagccgg cgcacgcgtt tctccaggcg cgagaggggg cggagtgcaa gttccgcccg
    21421 gtccgccagc cgtgttcaat cgtgtcgccg ttccaggcga actgttccgc caggccgaat
    21481 gactccgacc ctggcgaacg agcccatcac tggctcgtat catcaaattt atagcgagta
    21541 ttgactatgg ccaaacaatt cgatgtcgtc gtcattggcg gcggtcccgg cgggtatgtg
    21601 gccgccatcc gtgccgcgca actgggcctg tcggccgcct gcgtggatgc atggagcaat
    21661 gaacagggca aaccggcgtt gggcggcacc tgcaccaata ttggctgcat tccctccaag
    21721 gcattgttgc aatcgtccga gcactacgaa caggcgggca cgcatttcgc cgaccacggc
    21781 atcgaagtcc aaggcctgaa gctcgatctg ggcaagctgc tggcgcgcaa aagcaccgtc
    21841 gtcaagcaga acaatgacgg catccagtac ctgttcaaga aaaacaaggt gacgttcttc
    21901 cacggcacag gggcgttctc cgcacaggtc gatggtggct ggtcgatcaa ggtttcgggg
    21961 tcgtccgatg aggaattggt cgcccgtcat gtcattgtcg ccaccggctc cacggcgcgc
    22021 gcgctgccgg gtctgccgtt cgatgaaaac gtggttctgt ccaacgacgg cgcgctcagc
    22081 atcgatccgg tgcccaagaa gctcggcatc atcggtgccg gggtcatcgg cctggaaatg
    22141 ggcagcgtat ggcgccgcct gggggcggag gtcaccatac tggaagccat gccggacttt
    22201 ctcgcggccg tcgaccagca ggtggccaaa gaagcccaga aagtctttgc caagcaaggg
    22261 ctggtcattc ataccggtgc caagatcggc gaggtcaagg ccacggccaa gtccgtcacg
    22321 gtcccctgga ccgatgcgtc gggcgcggaa cagaaactgg tggtggaccg gctcatcgtg
    22381 tcgattggcc ggcttcccaa cacgactggg ctcggtgccg acgtcgtggg cctgaagctc
    22441 gacgaacgcg gcttcgtggc ggtggatgac gactgccgca ccaacctgcc caacgtctgg
    22501 gcggtgggcg acgtggtgcg tggccccatg ctcgcgcata aagcggaaga agaaggcgtg
    22561 gccgtggccg agcgcatcgc gggccaacat ggccacgtga acttcggcac cattccctgg
    22621 gtcatctaca ccactccgga aatcgcctgg gtcggcaaga ccgagcagca gctcaagacc
    22681 gaaggccgcg attaccgggc gggctccttc ccgtttctgg ccaatggccg ggctcgcgcg
    22741 ctgggcgaca ccacgggttt cgtcaaggtg ctggccgacg ccaaaaccga tgaggtgctt
    22801 ggggtgcata tcataggccc tgtggcgtcc gaactcatct ccgaggctgt caccatcatg
    22861 gagtttcgtg gtgcatccga agacatcgcc cgcatctgcc acgcgcaccc cacattgtct
    22921 gaagccgtca aggaagccgc cctcgcggtt gacaagcgcg ccttgaattt ttgatttccg
    22981 cgtgcggggc caggtgggac atgttgtttt ccctcccgcc tggtgcgccg cgccttctat
    23041 gaacgttcgc gagtattacc agaatgcgct cgatgagcgc ggctatcagt ccgacgaggc
    23101 ccaactggcc gcggtggagc gtctgcagcg gtattacgat gactgggtcg cgttcaaacg
    23161 ggctcgatcc accacgctga ggaagatatt ccgccggccc gatgtgccac gcggggtcta
    23221 cttgtggggc ggcgtggggc gaggcaagag ctttctcatg gatgcgttct acctgacagt
    23281 gcccgtgcgc cgcaagaccc gcatccactt ccacgagttc atgcgcggcg tgcatcggga
    23341 actggacgag gtcagggggc aggcggaccc cttggatgtg gtcgcaaccc gcgtggccaa
    23401 acgctatcgc ctgatctgtt tcgatgagtt ccatgtctcg gatgtggccg acgcgatgat
    23461 tctgcatcgc ctgctgctgc gcttgttcga gctgggcaca tcgtttgtga tgacgtccaa
    23521 ttacgagccg tccgctctgt atccggacgg cttgcaccgc gaccgtatcc tacccgcgat
    23581 caagcttctc gaagagcgca tggatatcct caatgtcgat actgggatcg attaccggcg
    23641 ccgtacgatg gcccaggttc gcacttatct gactcccatt gatgacgcca ctcgcgacga
    23701 gctgcgcgaa aacttccggc gtctgtccga caccgcgccg cagaagccgg tgctgcatat
    23761 cgaacaccgc gacatcaagg ccgtggcgct cggaggcagc attgtgtggt tcgatttcgc
    23821 cacgttgtgc ggcggtcccc gctcccagaa cgattacctg gagctcgcca gccgtttccg
    23881 cactgtgatc ctgtcggatg tgccacgcat ggacgcgcgc aacgcttcag aggcgcgccg
    23941 cttcacgtgg ctgatcgacg tgttctacga tcaccggatc aaactgatcg tgtcggcgca
    24001 gtgtccgccc gaggagttat atactcgggg accgatggcc aacgaattcc agcgcacggt
    24061 gtcgcgcatc atggagatgc agacgctgga atacctggag gccgaaaggc gccaggccgt
    24121 tgtgctgtga gaacccgccc cacgcattga ccggcggccg cggcgaatcc ggctcgttcc
    24181 caaaccctaa ccaccgtcaa ggctcatgaa tacccgtgtt ctgaccggca ttacgacctc
    24241 cgggacgccc cacctgggca atttcgccgg cgccatccgt ccggccatac agaccagcac
    24301 gcatcccgat gtggacgctt tttttttcat ggctgattat catgcgctga tcaagtgcga
    24361 cgatccggcc cgcatcagcc ggtcgcgtct ggagatcgcc gccacatggc tcgcggccgg
    24421 gctggacgcc gacaaggtca ccttctatcg gcagtcggac atccccgaga ttcctgaact
    24481 gtgctggatg ctgacgtgcg tgaccgccaa aggcttgatg aaccgcgccc atgcctacaa
    24541 ggccgcggtg gatcaaaaca tggccagggg cgaggagccc gacgacggcg tcaccatggg
    24601 cttgttctca taccccgtcc tgatggccgc cgacattctc atgttcaacg cgaacaaggt
    24661 gccggtcggg cgcgaccaga tccagcacct ggaaatggct cgggacatcg cccagcgctt
    24721 caatcacctt tacgggaagg gggtcgatta tttcgtgctg cccgacgtcc agatcgatga
    24781 cgaagtggcc actctgccgg ggctggacgg gcgcaagatg tccaagagct acgacaacac
    24841 catcccgctg ttcgaaggcg gcgcgaaggc gacgcgggat gcggtcatgc gcatcgtcac
    24901 cgactcgcgc aagccgggtg aacccaaaga cgcggagggc tcggcgctct acacgattta
    24961 tcgcgccttc gcccagcccg aggagtcggc acggttccgc cgcgaactcg aagacggcat
    25021 gggatggggg gacgccaagg acgccttgtg ccgccggatc gaggccgagc tcggtgccat
    25081 gcgcgaacag tatgcgtcgc tcatggctca tcctgatcgc atcgaggaca ttctccaggc
    25141 gggcgcggac aaggccaagc gcctctccac accgttcatg caacgtctgc gggaagcggt
    25201 cgggctggtg cgtggccgcg cctcccgtcc ggtggccgag gcaggcaaga agggcgaacg
    25261 gcgggcccgg ttcgtcagtt ttcgcgacga cgacggcgga ttccggttcc ggcttttggg
    25321 gagtgatggc gctgagctga tgttgtcgcg gcccttcggc gatcccagac aagccggttt
    25381 ggccatgcgg cgcctacggg atgagtcgct cgacgcgctc agcattgagc gtgacggggt
    25441 ggtgacggtg gtggttgatg attccccgat aggcgagttc gacagtgcgt tacgggaacg
    25501 cgtgtggcag gcgctggctg aattggccga caagtaggcc gcgggcggag ttcagggttg
    25561 gttggtttcc cgcacccgcg ctgaccaagc cgtcaggcaa cggtatcgac gcgcactagt
    25621 gccgttccgc gccgacctgt gtcaggcggc gtcgggcgcg atggtcctgg cgtgggcccg
    25681 gccgcgcaac cattccagcg tcaacagcag cagcactgaa aacacgatca atatcgtggc
    25741 gaccgccgcg atgaccgggc tgatgttctc acggattccc gtgaacatct ggcgcggcaa
    25801 cgtgacttgt tccggaccgg cgaggaaaag cgtcaccacc acctcatcga aagaggtcga
    25861 gaacgcgaac aaggctcccg acaccacacc cggtgcgatg atgggcaggg tgatgccgaa
    25921 gaacgtgcgc accggccccg cgccgaggct gtggctcgcg cgggtcaggt tctggtcata
    25981 gccctgcagc gtggcggcca cggtcgtcac cacgaatggc gcgcccaatg cggcgtgggc
    26041 cagtatcagc ccgatgtagc tgctggtcag cccatagggg gcgaagaaga gatagatgcc
    26101 cacgcccacc accaccagcg gcacgatcat gggcgaaatc aacaccgcca tcagcaagcc
    26161 cttgccacgg aattccgtgc ggtgcagtcc ggccgcggcc agcgttccca atatggtggc
    26221 gatgagcgtg gcggatggcg cgacgatgaa gctgttcttc gcggcccgta tccaatcgtc
    26281 ggagtggaac agcgtgtgat accacttgaa cgaaaagtcg tggatggggt agagcagcag
    26341 cgaactttca ctgaatgaaa gcggcatgat gaccagaatc ggcagcaaca ggaaaagcag
    26401 gatcagcacg cagtagtatc gctgcacgtg gaaccaggcc ttttcgatgc gggacacgta
    26461 gggtgggaag cgcggtggct gtttcttcat gtgctcaccc tagtccgagt tcgcggccgg
    26521 atagccgccg gtacagcgaa tagagcagca gtgtgcccag cagcaggatg aaccccagtg
    26581 cgctggccat gccccagttg attgtctggt tggtgaaata ggccacgtag tagctgatca
    26641 tctggtcgct cgcgccgccc agcagggctg gcgtgatgta gtacccgacc gcgatgatga
    26701 acaccagtag cgtccccgcg ccgatgccag ggtacgtttg cggcacgtac acccgccaga
    26761 acgctgcgaa cggatggctt cccagggaga tggcggcgcg ctggtaggtg ttgggcacgg
    26821 attgcatcac gctgtagatg ggcagcagca tgaacggcag cagaatgtgc gtcatggcga
    26881 tgtagacgcc cgtgcgattg aacagcagat caaacggcgt gttggtgagt cccacgctca
    26941 tgagcgcctt gttcaccagc ccctgtcgtt gcaggagcac gatccaggcc gcgatccgca
    27001 cgaggatcga ggtccagaac ggaatcagca cgcagatcat cacaagattg gccgtgcgcc
    27061 ttggcagctt cgagatccag taggcaagcg gataacccag caccagcgtg agcagcgtga
    27121 cgaccaggct gatcacgaat gtgcggccgt atatgtcgcg gaacgccgac gagccggccg
    27181 gaacatggac gatgtggccg ttcatgtcgc gcttcagatc catggacgcc agcaggtaat
    27241 aggacgagta ccgcgaatca ttgtcgatga tggcgcgcca gtattcgggc tgggaccagc
    27301 gtttgtctat gtggagaaaa cggtccttga tggcctggga cgggtctggt gtggaggcgg
    27361 tggtgttcga ggcagcaagc ggttgcgcgg ctggcgcatc gtcggcaccc aggtggcgca
    27421 tggtcttgaa caatagcgac cggtagcctg gaatctcata gttcaggcgt cgagccagtg
    27481 tgcccgcgtt ggcggtgttc cggactttcc ccagatcggc gcccagggct tcaaacgcca
    27541 cagagggtgg tgtcgatgtc ctgtcccagg atttcagcgc caccaatgtt cgcggcaggt
    27601 tgtggatgac ctcgggattt gccacggcgc gttcgagcaa tgccccgatg gggatgacga
    27661 agatcaccag gaggaacagc gccagtggcg cgaccagcag aaacgcgcgc agcttgcgtc
    27721 ggcgctcggc cctgcgcatc gccagccgga tcgcatgggt atcggtttcg gaaagggtag
    27781 ccgtcatgcc ggtgttcgct cgagggaggg cccgccgcct gcgcggcggg ctggcatcgg
    27841 aaaagtcacc gacctggtta ttgtgtggac caggcggtga agcgctgttc gagttcctcg
    27901 ccgtgatcgg cccagaagtt atagctggag agcagggcgt tcttgccgtt ctcgggtgaa
    27961 ttgggcagtt cagccagaat cttcggactg agtttcccta tggcgtgctt gttgacgggc
    28021 ccgtaggaaa tctgttcggc gtaggcggcc tgggggccag gcgacgtggc gaacttgatg
    28081 aattgctcgg ccagagcctt gttgggggag cccgtgggaa tgacccaata gtccagatcg
    28141 tagatattct gattccagac ggtcttcagc gattggtgat cgtctttgtt ggcggcgctg
    28201 atgcgcccgt tataggcggt ggacatgacc acgtcgccgg cggccagcat ttgcgggggt
    28261 tgcgcacccg cttcccacca ctggatgtac ggtttgagct cggtgagttt cttgaaggcg
    28321 cggttcacgc cctcgggcgt gcccaatact ttgtagacgt cctttggcgc cacgccgtcg
    28381 gccatgagcg cgaattccag gttgtagcgt gcacctttgc gcatgccgcg tttgcccggg
    28441 aatttcttca cgttccagaa gtcggcccag ctcttgggcg cggtcttcag ggtcttgccg
    28501 ttgtaggcca gcacggtgga ccacacgaac gctccgattc cgcattcacg cacagccgcg
    28561 gggatgaagt cggacttctt accgatatga tcccagttca gtttctcgta gagcccctcg
    28621 tcgcagccac ggcccaggtc gccggtctcg acttcgacgg cgtcccacac gacattgtgc
    28681 gtgtcgacca tggccttgac cttggcctgc tcgccgttat aggcaacggc aatgatgttc
    28741 ttgcccattt tcttctcgag cggcttgatg aaggcgacct tctgggcgtc cccgttggca
    28801 ccaccgaagt tcacgacggt caggtcgcgc gcgtgggctg tgccgactcc catggcggcg
    28861 atggcggata cgatggcgag agaacagagt gtcttgctgg ttttcatgga ttgtctccgt
    28921 tttggtttgg tatgtgtgac acgcgggtgc tgcgggaagt gatcgatggt gtgatgagga
    28981 gggcgtcagg ggctgaagac cctggcgtgt tcggtgggga tggaaagcag tatcgagtcc
    29041 cccgctctgg cgcggccaag ggcgtctttc tggtccaggg gaagcttgac gaacaacgtg
    29101 gtcgactcca gcgcctggca acgcagccgg atgtggtcgc caaaatagac ctgatccagc
    29161 gcctggacgc ggatcgcgtt ggtggtttgc gatccatcgg cgatgcgcaa tcgttcgggg
    29221 cggatggcaa gcacacaggc cgcaccgacc cgcagcgcgc cgatgttcag cgcggtgatc
    29281 aaggtgtcat cggggagccg cacctggcac cggccgtcct gttccgactc gatcacgcca
    29341 tcgatctggt tgcagtctcc cacgaagccg gcgacgaacc tgttttgcgg ctcttcgtac
    29401 agtgtgcgca ctggcgaaat ctgctgaatc acgccttggt cgaacaccgc cacgcgatcg
    29461 gacatggtca gcgcttcgct ctgatcgtgc gtgacataga cgaacgtgac acccagggat
    29521 cgatgcaagg ctttgagttc gagttgcatg tgctcgcgca actgtttgtc cagggctccg
    29581 agaggttcgt ccatgagaac gagcttggga tcgaatacca gggcgcgcgc cagcgcgatg
    29641 cgttgttgct gcccaccgga caattgaccc ggataacgct tcgcgaacgc actcatctgc
    29701 accatggcca gggcctcggc gacacgcttg gcgcgttcgg cggtcggcac atggcgaacc
    29761 ttgagcgggt agtgcagatt cttttcaacc gtcatgtgcg ggaacaaggc atagttctgg
    29821 aacaccatgc cgatgttgcg tttgtgcgga ggcacgtcgt tcaggcgttg accgtcgaga
    29881 aagatgtcgc cagcggtcgg aaactcgaac ccggcgagca tcatcaggca agtggtcttg
    29941 ccagagcccg atggccccag cagcgacagg aattcgcccg gccgtatgtc caggttcaga
    30001 ttcttgacca caagcgcctc gccgtcgtag gacttctgca cgccgcgaaa actgactagg
    30061 ggttcggaag gggtgatggc cacgtggtgg tctcttctgg gctgtgtgtt ggcggatggt
    30121 gtcgtcagct tcgaatcgct tcggacagga tgtgcagccc ttcatcgagc accgtgtctt
    30181 cgatggtgag ggggaacaga aagcggatca cgttaccgtg tacgccgcat gtcaacagaa
    30241 ccaggccgtt ttgcaaggcg cgtttctgaa tgcttgcggc tagttctgga ttgggccggc
    30301 gatcatcagg atgatggaat tcgaccgcga tcatggcccc caggccgcgc acttcggcga
    30361 tggccggcgt tgacgccgcc agatcgttca acgtggtgcg cagcttggcg cccatgatgt
    30421 tggcgcgctc gaccagtttt tcggaatcga tgatgtcaag caccgcgagc gccgatgcca
    30481 cggccagggg gttgcccgcg taggtgccgc ccagcgcgcc gggcgctggc gcgtccatga
    30541 tctcggcacg gccgcatacg ccggaaaggg gcatgccgcc cgccaggctc ttcgccatgg
    30601 tcatgagatc gggtatcacg cccgagtgct ccatggcgaa catcttgccg gtgcgcccga
    30661 accctgtctg gacctcatcg gcgatgagca ggatgccgtg ctgatcgcaa agagcgcgca
    30721 gcgcccgcag gaattccggc ggtgccacgt aaaacccgcc ctcaccctgc acgggctcga
    30781 caatgatggc ggccacgcgg cgtgggtcga tgtcagcctt gaacagggcg gcgatcgcgt
    30841 ccagagaatc ctgcacgctg acaccatgca gggcgttggg gaacggcgcg tggtacacgt
    30901 cgccagggaa tggtccgaaa cccagcttgt agggggcaac cttgcccgtg agcgccaatc
    30961 ccatgaacgt gcggccatgg aaggcacccg agaaggcaat gacaccgctg cgccccgtgg
    31021 cggcacgcgc gatcttcacc gcgttttcga cggcctcggc acctgttgtg aagagtgctg
    31081 ttttcttctg gtgatcgccg ggggcgcgct cgttgatttt ctcagccaac tccacgtagc
    31141 tggaatatgg cgaaatctgg aacgcggtat gggtgaactt gctcaactgc tgggaaatgg
    31201 cggcgaccac gcgcggatgg cagtgcccgg tgttggtgac cgcgataccc gctgcgaaat
    31261 cgatgtagcg gcgcccttga acgtcccaga gttcggcatt gagcgcgcgt tccgtgtaga
    31321 agtcgcaaag aatgccaatg ccgctgggcg tcgcgttgga tttccgggtc tgccaggatt
    31381 gattgtccat gatgatcagt tggggcgtgt tgagggttgc gcgatgatgt ggtagcggcc
    31441 attatgccga cagtctggtc tacaatctag agccattatt gaaaatttca tggagccaat
    31501 catgagttca ggtttcttca ccctggccga ctggttgcag caatggctcg atgtcggcac
    31561 cggcgaaccc gcctatcggc agttgtatcg cctgatacgt ggcgcgattc tggatggaca
    31621 tctgggggcg ggtgtgcggc ttccgtcctc acgcgcgctg gccgtggatt tgtcgatcgc
    31681 tcgcaatacg gtgcttcagg tctacgagca actcgaaatc gaaggttacg tcacggccgg
    31741 cacgggccga ggcacgttcg tggtggatat cagccaggaa atgtgcaatg acgcgaagca
    31801 ctccgcgccc cgaatcgcgc cacccggaac gtccacgcgc ggattgtccc cccgtgggcg
    31861 caagctggtt gagcaggctg ggttttccaa tcggcagtgg ggggcgttca tgcccggggt
    31921 tccggacgtg gccgaattcc cgtccaaggt atggaaccgc atccagagcc ggcattggcg
    31981 cagggcgccg accgaactcc tgacctatgc tcccgcaggg gggtatgagc cattgcgcca
    32041 cgaagtcgcc aactacctga acagtgcgcg atcggtcaac ggtggcgctg gccaggtcgt
    32101 catgaccacg ggtattcatc aaggcgttga cgtggtcagc cggttgctgt gccaacccgg
    32161 ggatctggtc tggatcgaag agccgtcata ttgggggata cgcaatctcc tgctttcctc
    32221 cgacatggaa gtggtttccg tgccggtgga cggcgagggc atgtgtttgt cggccgccga
    32281 cttgtcccgc ccgcctcgtc tgatcgtggt gtcgccgtcg caccagtatc cgttgggtat
    32341 ggtcatgagt ctggcgcggc gacggatgct gcttgaatac gcgcgccaga acggctgctg
    32401 gatcatcgag gatgactacg acagcgagtt ccgtttcggc agtcggccac tgccttcttt
    32461 gcagggcctg gatgacagcg gcttggtgct ctacgcgggc agtttcagca agacactgta
    32521 tcccgggctg cgcgtgggat atctggttgt gccaggcccg ctcgcggggc cattcgcgaa
    32581 cgccatcgcc gagctgtatc gcgaggggca attgatcacg cagtcgatac tcgccgagtt
    32641 catccgggag gggtttctgt cctcgcatat ccgtcgtgtc cgtaacttgt atgccgagcg
    32701 gcgcgtggcg ctgatcgccg cggtttcccg gcatttcggc aacgatctgc acatctatgg
    32761 cggcgaggcc ggcctgcacc tcgtgctggg tctgcctccg gaggtcgatg acgtgctggt
    32821 ggcgcgggaa gcgtttgtgc ggggtgtcat cgcgcgccca ttgagccgtt attacatgaa
    32881 tccctcgcat gtgcgccaag ggctgttgct tggctacgca tcagtgccat tggaccgcat
    32941 agacgcggcg ttcgatgtgc tggccaccgt gattcaacgg cacctgctcg ccgcctctgg
    33001 ccgaagtgta aacgcatgaa aagcaagggt tgcatattga agaaaattgt gccaagctct
    33061 tgtgcagatg gaatcagtgc agttggctga ttggttccat caaaactgcc gcgaggctga
    33121 tgactcctac cgaccgcggc ggcgcggtat cacggtgggg aattatgcga acccacctat
    33181 taggtgggtt ttttgtcgtg cgtttcccga aatggagccg tattgaatgt tccgcccacg
    33241 ctcatgcgtg atctgtgggt ttattggcgg acttcgacgc ttgtttatgg gagaaatcaa
    33301 atgagcgata caacagtcac gaacgaaggt tcgaagagcg ggtcggttcc cgaggcggtc
    33361 gcgggcttgg cggtggtcat cctggcgatc ctgggtttgg cgggtgtcag cccaacgttt
    33421 ctggtggcga tcgccaccat cgtgttcggg gtcgaattgt tgctgaatgg ggcgtc
//
LOCUS       DASTNK010000109         4939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1378386, whole genome shotgun sequence.
ACCESSION   DASTNK010000109 DASTNK010000000
VERSION     DASTNK010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378386"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /locus_tag="VFR20_08200"
     CDS             <1..169
                     /locus_tag="VFR20_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HEU0230539.1"
                     /translation="RISNFLVWQLAYTELYFTDSYWPDFGAKELDAAFEWYRTRERRF
                     GRTSEQVAKGR"
     gene            183..1043
                     /locus_tag="VFR20_08205"
     CDS             183..1043
                     /locus_tag="VFR20_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU0230540.1"
                     /translation="MLGQRVITAVILLAILLGALLFTSQPFVLVGLLVIVAACALWEW
                     LRLTWPTRPSHAPMVCALVFALALCLIALSWHGADPQEWGTQLRRLARHWLFPLVVVL
                     WVVGGLGMVVRGQVSERSGAVCLSVFGVLAVLAAWLALVELFLRRGPWFLVSLLALVW
                     VADIAAYFSGKAFGRHKLAPAVSPGKTWEGAGGGVVAAAAWVAISSFWPGSLGEALVR
                     HWSWGGALIIAVGLAALSIVGDLFESLLKRRAGVKDSSGLLPGHGGVYDRIDALLPVA
                     PLALLLVGMR"
     gene            1040..2239
                     /locus_tag="VFR20_08210"
     CDS             1040..2239
                     /locus_tag="VFR20_08210"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEU0230541.1"
                     /translation="MTMQHLCILGSTGSVGENTLDVVARHPDRFTVHALSAYSRMERL
                     AAQAAGSGARVVVVPDAAARRRFQDAWAGVGRRPEILVGAQALADVATDSHVTTVMAA
                     IVGAAGLPSALAAARAGKRILLANKEALVAAGGLFMKSVREHGAALLPIDSEHNAIFQ
                     CLPTVHGAQARVERPVGVRRLLLTASGGPFRTTALSELDDVTPDQACAHPNWRMGRKI
                     SVDSATMLNKGLEVIEAHWLFAMPVADIQVVVHPQSTVHSMVEYLDGSILAQLGQPDM
                     RTPIAYGLGFPERIEGGVGLLSLATLGRLDFEEPDLARFPCLKLSFEAARAGQASCVT
                     LNAANEVAVAHFLDRRIRYTEIPRVIERSLARQAGRPTPSLDDLDDILALDGETRTFA
                     SELCLVS"
     gene            2328..3659
                     /gene="rseP"
                     /locus_tag="VFR20_08215"
     CDS             2328..3659
                     /gene="rseP"
                     /locus_tag="VFR20_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU0230542.1"
                     /translation="MLLTLLAFAFALGVLITFHEFGHYWVARRCGIKVLRFSVGFGKV
                     LLRRTDRHGTEWALSAIPLGGYVKMLDDPEPGATAREQRQAFNRQPVGKRMAVVLAGP
                     IANLLLAAILYTLLGLVGRVEPQPIVAAPPANSSAAKAGFKQGDRIVSIDHSVVRSWG
                     EARWHLMDALTSGGDVRVVVDPGHGAMREYTLHLEAPADPSGNADLMAVAGLRLAPGK
                     PVIDRVTKGGAAAKSGLQADDIVIGVAGQADPTVNALVGYIQKHAGEPVALDVVRGGK
                     SMSVVVTPEAESVKGATIGRIGALISADLPVTNVRYGFVDSVLNGVERTAETTWLSLK
                     MMGRMVIGSVSLRNISGPVTIADYAGQTARIGLAAYLGFMALISVSIGVLNLLPIPML
                     DGGHFMFCLLEAARGRPVSDRWRLKGQQIGFGLLMALMVLAFFNDFTRLFS"
     gene            3719..>4939
                     /gene="bamA"
                     /locus_tag="VFR20_08220"
     CDS             3719..>4939
                     /gene="bamA"
                     /locus_tag="VFR20_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEU0230543.1"
                     /translation="MLSRRNFGFTKRVLPVLMAAMLVPGLANAFSPFVVRDIRVDGLQ
                     RVDPGTVFTYLPVKVGETFTEADAAKAIQGLYGSGLFNDVKIDDQGGVLVVSVKERPT
                     IASVSFNGMREFDAKAIIKSLSQVGFGQGRVFDRSLLDRAIFELKQQYLSKGKYGVQI
                     NPVITPLPRNRVGVSFDIFEGEVARIKQITFVGNHAFSQGTLLDQLDLTTSGFMTWYT
                     DTDKYSREKLEGDVDRIRSFYLDRGYLEYSAEPPQVSISPDRHNIYITFTIHEGKPYK
                     IHSVKLAGNLLGLDDKIRKLITVKAGETFSAAKTNATAKAITDYLGSLGYAFANVNPN
                     PVLDRDKHEADLTFYVDPGRRVYVRRIQIGGNTRTRDQVIRREMLQQEAAWYDAASIK
                     QSRDRIDRLGYFNDV"
BASE COUNT          793 a         1530 c         1606 g         1010 t
ORIGIN      
        1 gcgcatctct aattttctgg tgtggcaact ggcctatact gaactctact tcaccgacag
       61 ctactggccc gacttcggcg ccaaagagct cgacgccgcg ttcgagtggt accgcacccg
      121 cgagcggcgc ttcggccgca ctagcgaaca agtcgccaag ggccgttagg ccagggagca
      181 ccgtgctggg gcaacgcgtc atcaccgccg tcattctgct ggccatcctg ctgggggcgt
      241 tgctgttcac gtcacagccg ttcgtgttgg tggggctgct cgtcatcgtc gccgcgtgcg
      301 cactctggga atggctgcgc ttgacctggc cgacgcggcc ttcgcatgcg ccgatggttt
      361 gtgccttggt gtttgcgctg gcgctgtgct tgatcgcact gtcctggcac ggcgccgatc
      421 cccaggaatg gggcacgcaa ctgcgccgcc tggcccggca ttggcttttt ccactcgtcg
      481 tcgtactctg ggtcgttggc ggtttgggca tggtcgtgcg tggccaggtc tcggaacgct
      541 ccggcgcagt ctgcctcagc gtgttcggcg tgctggcggt gctcgccgcc tggctggccc
      601 tggtggagct cttcctgcga cgggggccgt ggtttctggt gtccttgctc gccctggtat
      661 gggtcgccga cattgcggcg tatttttcag gcaaggcttt cggccgccac aaactggcgc
      721 ccgcggtcag ccccggcaag acctgggagg gcgcgggcgg aggagtggtg gcggcggccg
      781 cgtgggtggc gatcagttcg ttctggcccg gcagcctcgg agaggcgctg gtgcggcact
      841 ggtcatgggg cggagccttg atcatcgcgg tggggcttgc cgcgctgtcc atcgtcggcg
      901 acctgttcga gtcgttgctc aagcgacgcg ccggggtcaa ggattctagc ggccttctgc
      961 cggggcatgg cggtgtatat gaccgcattg acgctttgct gccggtggcg ccgctcgccc
     1021 tcttgttggt ggggatgcga tgacaatgca acatctgtgc attctgggtt cgaccgggtc
     1081 ggttggcgag aacacgcttg atgtggtcgc gcgtcacccc gaccggttca ctgtccacgc
     1141 cctgagcgcc tacagcagga tggagcggtt ggcggcgcag gcggccggct cgggcgcccg
     1201 ggtcgtggtc gttccagacg cggcggcgag acggcgcttt caggatgcct gggccggcgt
     1261 gggccgccgg ccggaaatcc ttgtgggcgc ccaggcgctg gccgacgtcg ccacggactc
     1321 ccatgtcacg accgtgatgg ccgccatcgt cggcgcggcc ggattgcctt cggccctggc
     1381 cgccgcgcgc gccggcaagc gtattctgct cgccaacaaa gaagcgctgg tggccgcggg
     1441 cggcttgttc atgaaaagcg tgcgcgagca cggtgcggcg ctcctgccca tagacagcga
     1501 gcacaatgcc atcttccagt gtctgcccac cgtgcatggc gcccaggccc gtgtcgagcg
     1561 cccggtgggt gttcggcgcc tgttgctcac tgcctcgggc gggccgttcc gcaccacggc
     1621 gttgtcggag ctggacgatg tcacgccgga ccaggcgtgc gcgcacccaa actggcgcat
     1681 gggccgcaag atttcggttg attcggccac catgctcaac aaggggttgg aggtcatcga
     1741 ggcccattgg ttgttcgcca tgcccgtggc cgatatccag gtggtggtcc acccacagag
     1801 cacggtgcac tccatggtcg aatatctcga tggctccatc ctggcgcagc tcgggcaacc
     1861 cgacatgcgc acgcctatcg cttacgggct gggcttccca gagcgcatag aaggcggcgt
     1921 cggcctgttg tcgctcgcca cgctgggtcg tctggacttc gaagagcccg atctcgcgcg
     1981 cttcccatgt ctgaaactgt ctttcgaggc cgctcgggca ggacaggcgt cgtgtgtcac
     2041 gctgaacgcc gccaacgaag tcgcggttgc gcatttcctc gatcggcgga ttcgctatac
     2101 tgaaatcccg cgcgtgatcg aacgaagcct ggcgcgtcag gcggggcgcc cgacgccctc
     2161 gctcgatgat ctcgacgata tattggcgct cgatggcgag acacgaactt tcgcctcgga
     2221 attatgtctt gtttcttagc gctcgcgccg atgcgccgaa tgctccggat cgacactagc
     2281 cgatcccgca ccggcgtgcc gaaacctcca acccggctcc tgttctcatg ttgttgaccc
     2341 tgctggcttt cgcattcgcg ctcggggtgt tgatcacctt ccacgagttc ggccactatt
     2401 gggtggccag gcgctgcggc atcaaggtgc tgcggttttc cgtgggattt ggcaaagtgc
     2461 tgctgcgccg tactgatcgc catggcacgg aatgggcctt gtccgccatt cccctgggcg
     2521 gatatgtcaa gatgctcgac gatccggaac ccggcgccac ggcgcgggaa cagcggcagg
     2581 ctttcaaccg tcagcccgtc ggcaagcgca tggcggtggt tctggcgggg cccatcgcca
     2641 atctgctgtt ggcagccatt ctgtacactc tgcttggcct ggtcggcaga gtggaaccgc
     2701 agcccatcgt ggccgcgcca ccggcgaact ccagcgccgc caaggccggg ttcaagcagg
     2761 gcgaccgtat cgtgtccatc gaccattccg tggtccgctc ctggggcgaa gcacgctggc
     2821 atctcatgga tgcgctgact tccggcggcg atgttcgagt ggtcgttgat ccgggccacg
     2881 gcgcgatgcg cgaatacaca ctgcatctcg aggcgccggc ggatccctcc ggcaacgcgg
     2941 acttgatggc ggtggcgggg ttgcgccttg ccccaggcaa gcctgtcatc gaccgtgtca
     3001 caaaaggtgg cgccgcggcg aagtccggct tgcaggcgga cgacatcgtg atcggcgtgg
     3061 cggggcaggc ggatcccact gtgaacgccc tggttggtta catacagaaa catgccggcg
     3121 agccagtggc attggatgtc gtgcgcggcg ggaaatcgat gtcggtggtt gtcacgccgg
     3181 aggcggaaag cgtgaagggc gccacgatcg ggcgcatcgg cgccctgatc agcgccgatc
     3241 tgcccgtgac caacgttcgc tatgggtttg tggacagcgt cctgaatggg gtcgagcgca
     3301 ccgccgagac gacctggctc tctctcaaga tgatggggcg catggtgatc ggcagcgtat
     3361 cgttgcgcaa tatcagcggc ccggtgacca ttgccgatta cgcgggtcag acggcgcgca
     3421 tcggtttggc ggcctatctc ggtttcatgg cgctgatcag tgttagtatc ggcgttttga
     3481 atctgttgcc catcccgatg ctggatggcg gtcatttcat gttttgcctg ctggaagcgg
     3541 cgcgtgggcg cccggtgtcc gatcgctggc ggctgaaagg gcagcagatc gggttcggcc
     3601 tgctcatggc gctgatggtg ctcgctttct tcaatgattt cacgcgcctt ttcagttagg
     3661 cgcgttgtgc cttaaaatcc ttcaccattt gaatgttaaa gctctgagga tacccaggat
     3721 gctgtcccgc cggaatttcg gattcaccaa gcgtgtttta cctgtcctga tggcggcgat
     3781 gcttgtcccg ggtctcgcca acgcgttttc cccattcgtg gtgcgggata ttcgcgtcga
     3841 tggcctgcaa cgcgtcgatc cgggcactgt gttcacgtac ctgcccgtca aagtgggcga
     3901 aaccttcacc gaggcggacg cggccaaagc catccagggc ttgtatgggt cgggcctgtt
     3961 caacgacgtc aagatcgacg atcagggcgg cgtgctggtc gtcagtgtga aggaacggcc
     4021 aaccatcgcg tcggtttcgt tcaacggcat gcgcgaattc gacgccaagg ccatcatcaa
     4081 gtccttgagc caggtgggtt tcggccaggg gcgggtgttc gaccgctcgt tgctggaccg
     4141 cgccatattc gaactcaagc aacaatacct gtccaagggc aagtatgggg tccagatcaa
     4201 tccggtcatc acgccattgc cacgcaaccg tgtgggtgtc agcttcgaca tcttcgaggg
     4261 cgaagtcgcc cgcatcaagc aaatcacgtt tgtgggcaat catgcgtttt cgcagggcac
     4321 actgctcgat cagcttgacc tgaccacctc gggcttcatg acgtggtaca ccgacaccga
     4381 caagtactcg cgcgagaaac tcgagggtga cgtcgatcgc atccgttcgt tctacctcga
     4441 ccggggctat ctggaatatt ccgccgagcc gccccaggtg tccatttcgc ccgatcggca
     4501 caatatctac atcacgttca ccatccacga gggcaagccg tacaagatcc acagtgtgaa
     4561 gctggccggc aacttgctgg gcctggacga caaaatccgc aagctcatca ctgtcaaggc
     4621 cggcgaaacg ttctcggcgg ccaagaccaa tgccacggcc aaggccatca ccgactatct
     4681 gggcagcctg ggctacgcgt tcgccaacgt caaccccaac cccgtgctcg atcgcgacaa
     4741 gcatgaagcc gatctcactt tctatgtcga tccggggcgg cgcgtctacg tgcggcgcat
     4801 ccagatcggc ggcaacaccc gcacccgcga tcaagtgatc cggcgcgaaa tgctgcaaca
     4861 ggaagccgcc tggtacgacg cggccagcat caagcagtcg cgcgatcgca tcgatcgcct
     4921 gggttatttc aacgatgtc
//
LOCUS       DASTNK010000110         4312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1380836, whole genome shotgun sequence.
ACCESSION   DASTNK010000110 DASTNK010000000
VERSION     DASTNK010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380836"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VFR20_08225"
     CDS             <1..400
                     /locus_tag="VFR20_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEU0230544.1"
                     /translation="VALLSRSNFGSDSSSSGPKMREALHLIREQAPDLEIDGEMHGDC
                     ALDEALRLRILPSTTLKGEANLLVCPNVDAGNIAYNLLKTAAGGNVAVGPFLLGVNAP
                     VHILTSSSTVRRIVNMTALAVVDANTPRAT"
     gene            411..1607
                     /locus_tag="VFR20_08230"
     CDS             411..1607
                     /locus_tag="VFR20_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU0230545.1"
                     /translation="MAAGSSPVGLVLTGGGARAAYQVGVLAGVMDILDPLRSPVMQNP
                     FPIICGSSAGAVNATAIACRADTPHLAVDRLCNLWENLHTEMVYRSDAGDLLRTGLHW
                     LAMLAFGWAMPAARRRRPRSLLNNAPLARLLTKTLNFRRLHGNIAKGNLQALAITASG
                     YTSGEHLTFYQSDGSIKPWRRTLRRAVPCSIGVEHLMASSSIPFIFPAQALTFDGETE
                     WCGDGSMRQLAPVSPAIHLGAQKVFVIGTGHLDDTHPEKRNVNPDYPSLAQIGGHALS
                     NIFLDSLSMDIERLERINELLAQMPPNGLKSHSLRPVSSFMITPSRSLDEIAMENLEQ
                     MPRAARTLFKVLGVSSNSQASAGGALISYLLFETGYTKALIDLGRADSLRRSEEVKAF
                     FKETQE"
     gene            1604..2074
                     /locus_tag="VFR20_08235"
     CDS             1604..2074
                     /locus_tag="VFR20_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEU0230546.1"
                     /translation="MTQAKSLQKKLQKGGLLDVATVSQDEVLEAARELGMTLFVVDCD
                     RARSRSAVLRAIAKAVDFPEFFGGNLDALYDCLCDTVQDQKVGVFLWLHSLHSGDPAL
                     VEDAEAIISVCGDAAEFASNNERLFAFGVEHSGRHPEPEPGVSPTPYSGGSEQE"
     gene            complement(2170..2907)
                     /locus_tag="VFR20_08240"
     CDS             complement(2170..2907)
                     /locus_tag="VFR20_08240"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU0230547.1"
                     /translation="MTSPRFYCPAPIAPNQTMALPADLAHHAIRVLRLASGTPVVLFD
                     GRGGQYPARLVIDGKTGVAEIGARQEVEAELAGELILVQGIVSGDKMDWIIEKAVELG
                     ASRLVPVAARRSVPLLKGERLEKRRLRWARIVQAASEQCGRNRLMHIDAPRTLDDYLG
                     EPGAPAPDQILCCHPDADRALSQTLAPTARRIALLVGPEGGWSDDELAVATAHQAQPV
                     RFGTRVLRTETAGLALLTAVATLKGWQ"
     gene            3110..3658
                     /locus_tag="VFR20_08245"
     CDS             3110..3658
                     /locus_tag="VFR20_08245"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HEU0230548.1"
                     /translation="MRFILSFLLLGAALSFGASQAQAADRMAPGLWEMTAQSDALKGL
                     PVIPPEQLAKLRALGIQIPQIANGSATTHTCITPEMARRADMAPAAGASKMGCSAHNV
                     RFDGNRFSADLVCDNDRAKGRGKVSGTFSGNKAFTADIQFTGTVQGVPVNQQGTASGR
                     WLGNDCGSVKPLNLPPEPRQGK"
     gene            3774..>4312
                     /locus_tag="VFR20_08250"
     CDS             3774..>4312
                     /locus_tag="VFR20_08250"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809443.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU0230549.1"
                     /translation="MTTSSVPDISMANAIRALAMDAVQKAKSGHPGAPMGMADIAQVL
                     WARHLRHNPADPTWPDRDRFVLSNGHGSMLLYALLHLSGYDLSIDELKNFRQLHSRTP
                     GHPEVGITPGVETTTGPLGQGVSNAVGMALAEALLAAEFNRDGHKVVDHFTYAFVGDG
                     CLMEGISHEACSLAGTLKLS"
BASE COUNT          751 a         1358 c         1364 g          839 t
ORIGIN      
        1 ggtggcgctg ctctcgcggt cgaatttcgg cagcgacagt tcgtcatccg gtccgaagat
       61 gcgcgaagcg ctccatctca tccgcgagca ggcgcccgat ctcgagatcg acggtgaaat
      121 gcacggtgac tgcgcgctcg atgaggcgct gcgcctgcgc atccttcctt ccaccacgct
      181 gaaaggcgag gccaatctgc tggtgtgccc caacgtggac gccggcaata tcgcgtacaa
      241 ccttctgaag accgcggcgg ggggcaatgt ggcggtgggg ccattcctgt tgggtgtcaa
      301 tgcgcccgtg cacattctca cgtccagttc cacggtgcgt cgcatcgtga acatgacggc
      361 gttggccgtc gtcgacgcca acacgccacg cgcgacttga tcggtgccgc atggctgcgg
      421 gaagctcgcc agtcggattg gtactgacgg gtggcggcgc gcgcgccgcc tatcaggtcg
      481 gggttttggc cggggtgatg gacatcctcg atccgttgcg ctcgccggtc atgcaaaatc
      541 cgtttcccat catctgcgga tcatcggcgg gggccgtcaa cgcgacggcg atcgcctgcc
      601 gcgccgatac cccgcatctg gccgtggatc ggctgtgcaa cttgtgggag aacctgcata
      661 ccgagatggt gtatcgctcg gatgcgggcg atttgctgcg cacaggactg cactggctcg
      721 ccatgctcgc gttcggctgg gccatgcccg cggcgcgccg caggcgcccc cggtcgctgc
      781 tcaataacgc cccactggcc agactgttga ccaagacgct gaatttccgg cgtctacacg
      841 gcaatatcgc caaagggaac cttcaggcgc tggccatcac cgcgtcgggc tataccagtg
      901 gcgaacatct cacgttctac cagtccgacg gctccatcaa gccatggcgc cgcaccttgc
      961 ggcgcgcggt gccatgctcc atcggtgtcg agcacctcat ggcgtccagt tccattccct
     1021 tcatctttcc ggcccaggca ctgaccttcg acggcgaaac cgaatggtgc ggtgatgggt
     1081 ccatgcgcca actggcgccc gtcagcccgg ccatacatct cggggcgcaa aaagtattcg
     1141 tcatcggcac gggccatctg gatgacaccc atccggaaaa acgcaacgtc aaccccgact
     1201 acccgtcatt ggcacagatt ggcgggcatg ccttgtcgaa cattttcctt gatagcctgt
     1261 ctatggatat cgagcgtctc gaaaggatca atgaactgct tgcgcagatg cctccgaatg
     1321 gtttaaaaag ccattcgctg cgtccggtga gttccttcat gatcactccc agccgttcgc
     1381 tcgacgaaat cgccatggag aaccttgaac aaatgccgcg cgctgcgcgc acccttttca
     1441 aggtacttgg tgtatcgtcg aattcgcagg cctcggcggg cggggcgctg atctcatatt
     1501 tgctgttcga aacaggctac accaaggcgc tcatcgatct cgggcgcgcc gacagcttgc
     1561 gccgcagcga ggaagtcaaa gcgtttttca aggagacgca agaatgaccc aggcaaagtc
     1621 attgcagaag aaactgcaga aaggtggctt gctggacgtg gccactgtgt cgcaggatga
     1681 agtcttggaa gccgctcgcg aactgggcat gaccctgttc gtcgtcgatt gcgaccgggc
     1741 ccgcagccgt tcggctgtgc tccgggccat tgcgaaagcc gtcgattttc cggaattctt
     1801 cggtggcaac ctggatgcgt tgtacgactg cctgtgcgat accgtgcagg accagaaggt
     1861 tggcgtgttc ctgtggctgc atagcctgca ttcgggggac ccggctcttg tcgaggacgc
     1921 ggaagccatc atcagcgtgt gcggtgacgc ggccgagttc gcctccaata atgaacgctt
     1981 gttcgccttc ggtgtcgagc actccggccg ccatcccgaa cccgagccgg gggtgtctcc
     2041 caccccttat tcgggtggga gcgaacagga atagcggcta accagtgtgc tgtctggtca
     2101 aggcctgttc acgcttcgaa tttgattgcg tgaaccggtc gggcggcaca ccggcctggc
     2161 ggggttccat cattgccacc ctttcagggt agcgaccgcg gtcagcagcg ctaggcccgc
     2221 cgtttcggtg cgaagcacgc gtgtcccgaa gcgcaccggc tgggcctggt gggcggtggc
     2281 cactgccagt tcatcgtccg accagcctcc ttcggggccg accagtaacg cgatgcggcg
     2341 cgcggtcggc gccagcgtct ggctcaatgc cctgtcggca tccggatgac agcacaagat
     2401 ctgatcgggg gcgggtgcgc ctggctcacc caggtagtcg tccagcgtgc gcggcgcatc
     2461 gatatgcatc agccggttcc ggccgcattg ttcgctggcg gcctggacga tacgcgccca
     2521 gcgcagacgg cgtttttcca ggcgttcccc tttgagcaat ggaacactgc gacgcgcggc
     2581 cacgggaacc agacgggagg cgcccagttc gaccgctttt tcgatgatcc agtccatctt
     2641 gtcacccgag acaatgccct ggaccagaat cagttcacca gccagctccg cttccacttc
     2701 ctgccgcgcg ccgatttccg ccacaccggt tttgccgtcg atgaccaggc gcgccggata
     2761 ttgaccgccc cgcccgtcga acaagacgac gggcgtcccg gaggcgaggc gcaacacacg
     2821 aatggcgtgg tgcgccaggt cggcgggaag cgccatggtc tggttggggg cgatgggtgc
     2881 cgggcagtag aagcgggggc tggtcatgta acgtgaatgg gcgtatcgtc ggatggtcgg
     2941 gcgccaagcc ggactgggcg gcttgcctgg gttgcgggtg tcactctttg tcagtgtcaa
     3001 ataccgattt tatccggccc gcctgtcgcg gccatctttc cggttgcctg gttcggcgct
     3061 ttgccgcctg ctcaacaatc ttgcttcacc gttttctcaa aggtacgcca tgcgtttcat
     3121 tctgtccttt cttctgctcg gcgcggcgtt gtcgttcggc gccagtcagg cacaggccgc
     3181 cgaccgcatg gcgccgggac tgtgggaaat gacggcgcaa tccgatgccc tgaagggcct
     3241 gccggtcatc cctccggagc aactggccaa attgcgggcg ctgggcattc agattccgca
     3301 gatcgccaat gggtcggcca cgacccacac ctgcatcacg cccgagatgg cccggcgcgc
     3361 cgatatggcg ccggcggccg gcgccagcaa gatgggctgt tcggcacaca atgtgcgctt
     3421 cgacggcaac cggttttcgg ccgacttggt ctgtgataac gaccgcgcca agggaagggg
     3481 caaggtgtcg ggcacgtttt ccgggaacaa ggcgttcacg gccgacatcc agttcacagg
     3541 aaccgtgcag ggcgtgccgg tgaatcagca gggtaccgcc tcggggcgat ggctcggcaa
     3601 tgattgcggc agtgtcaagc cgctgaacct gcctccggaa ccgcgtcagg ggaagtaacc
     3661 ggaatggcgc ccgcgtgggt ggccgttctc cgtgcgaagc ccggccaccc acgggggcga
     3721 ggggccgtcg gtggtaaaat cgcatgcttt gcaaacaccg gttcccctct ctgatgacta
     3781 catcgtccgt cccagatatt tccatggcga acgccatccg cgcactggcc atggacgcgg
     3841 tgcaaaaagc caagtcgggc catcctggcg ctcccatggg catggccgac atcgcgcaag
     3901 tgctctgggc gcggcatctg cggcacaacc cggccgaccc cacctggccc gaccgcgacc
     3961 ggttcgtcct gtccaatggc catggctcca tgttgctgta tgccttgttg catctatcgg
     4021 gctacgatct gtcgatcgac gaactcaaga atttccggca actgcactcg cgcaccccgg
     4081 ggcaccccga ggtcggcatc actccgggcg ttgaaaccac caccggcccg cttgggcagg
     4141 gggtgagcaa cgcggtcggc atggcgctgg cagaggcctt gctggcggcg gaattcaacc
     4201 gcgatgggca caaggtggtc gatcacttca cctatgcgtt cgtcggcgat ggctgcctga
     4261 tggagggcat ttcccacgaa gcgtgttcgc tggcgggaac gttgaagctg tc
//
LOCUS       DASTNK010000111         4612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1009212, whole genome shotgun sequence.
ACCESSION   DASTNK010000111 DASTNK010000000
VERSION     DASTNK010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009212"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..782
                     /locus_tag="VFR20_08255"
     CDS             72..782
                     /locus_tag="VFR20_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0230550.1"
                     /translation="MQNPAPEPDFAVLPVLPKVTRMRLHDTVVEHLRNFIVEGVLAPG
                     TKLNERELCDTLGISRTPLREAIKVLAAEGLIDIAPNRGATVSRMSEAEIREMFELMS
                     GLEAFSGELACARITPGELSEIKALHYTMLACHAQNDLPGYYAQNHKIHDRINLAAGN
                     SALRQIYISVNRRLQALRFRSNFQTAKWDQAVAEHEEMLKALEARDGARLAAILRQHL
                     LEKRDAVLQLLADDAPQH"
     gene            791..2155
                     /gene="mnmE"
                     /locus_tag="VFR20_08260"
     CDS             791..2155
                     /gene="mnmE"
                     /locus_tag="VFR20_08260"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU0230551.1"
                     /translation="MPSHSKEPIAAIATAPGRGGIGIVRVSGPDLDELVRALFDDMLA
                     PRKAHYRPFNDRHGEAIDAGVALYFKSPHSYTGEDVLELQGHGGTAVLRRLLERCLEA
                     GASLGIRHARPGEFTERAFLNERLDLAQAEAVADLIDASSTAAARSAMASLSGVFSTR
                     VNALAERIINLRMLVEATLDFPEEEIDFLRSYQAREKLTDIRSDLDTIIRQTRQSMAL
                     REGLYVVLAGQPNVGKSSLLNALAGDDIAIVTPIAGTTRDKVTQQIHIDGVPIHIVDT
                     AGLRETDDTVERLGMERTWAELEKANVILHMQDVGRRNDALDTAIVQRLPKGIPVLAV
                     LNKIDLAQKPEPPAAGSNELHISAKTGVGLDELKRRLLEVAGRDPGMESPWLARERHL
                     LALNNAGEHLALADEHAGHNDQVLDLFAEELRLAHGDLCAITGQFTSDDLLGEIFSRF
                     CIGK"
     gene            2311..2889
                     /locus_tag="VFR20_08265"
     CDS             2311..2889
                     /locus_tag="VFR20_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230552.1"
                     /translation="MGATAIHIDQFVHIRIIIGILLGLSISRLVTGLSRSVQTSASFR
                     IYPLHVVWIVYLLLAIVDFWWFEYGLLKIKVWTFGLYLFVIFYASIFVFISSILFPDR
                     GHGYVSYEKYFQGRRRPFYCMMLLLSVLDVVDSWIKGREHFASLGVDYLVVQAIEALG
                     ALVAIIVASRKYQMAYALTAVACEVYLLSKMF"
     gene            complement(2999..3592)
                     /locus_tag="VFR20_08270"
     CDS             complement(2999..3592)
                     /locus_tag="VFR20_08270"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU0230553.1"
                     /translation="MTTGPDDASLVETRIDGQTLVEGGFLTFRRDRVRLPDGAQASRE
                     YVVHPGAVVVIPLLDDGRVVVERQYRYPVGRVMTEFPAGKLDAGEDPLACAKRELLEE
                     TGYTATHWASAGAMHLAIAYSTEIIHIFFARGLRAGPRHLDQDEFLEMRTMGVEELLE
                     ACREGRVTDAKTLTCALWLQNVRTGAWKLDWRENPGA"
     gene            3746..4156
                     /locus_tag="VFR20_08275"
     CDS             3746..4156
                     /locus_tag="VFR20_08275"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEU0230554.1"
                     /translation="MDIGRMKDQHVQILTSISDLRELAHAGVAQNAAHIARELKAMGG
                     VIVTHLAAEDRVLYPSMKKQPNQELVRLCQQYQDEMSDLASAFKHFSHHWTNVSEIER
                     HPEQFREEANVVMKKLHTRIRRENTELYPVVEAL"
     gene            4293..>4612
                     /locus_tag="VFR20_08280"
     CDS             4293..>4612
                     /locus_tag="VFR20_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU0230555.1"
                     /translation="MSHAEPTLVQHLPDGPLDIVGDIHGELSALLALLVRLGYNSQGG
                     HPENRKLVFLGDLCDRGPDSVGVIQVVQRLVEHGNAQAILGNHELNLLIGDAKDGSGW
                     FFDER"
BASE COUNT          954 a         1486 c         1306 g          866 t
ORIGIN      
        1 ttcccaacca tttcgatctc gtgaagtaaa attcccggga tctcgcaata ctcgcgcaac
       61 actcgttttg catgcaaaat cccgcaccag aacccgactt cgctgtgctg cccgtgctcc
      121 cgaaagtcac gcgcatgcgc ctgcacgaca cggttgtcga acacctgcgc aacttcatcg
      181 tggaaggggt gctcgccccc ggcaccaagc tcaacgagcg cgaactgtgt gacaccctgg
      241 gcatttcccg caccccgctg cgcgaggcga tcaaggttct ggcggccgag ggcctcatcg
      301 acatagcacc gaatcgcggt gccaccgtct cgcgcatgtc cgaggcggaa attcgcgaaa
      361 tgttcgaact catgagcggc ctcgaagcgt tctcgggcga gctggcctgc gcgcgcatca
      421 caccgggtga actctcggaa atcaaggcgc tgcactacac catgctggcg tgccacgccc
      481 agaacgatct gcctggctac tacgcccaga accacaagat ccacgaccgc atcaacctgg
      541 cagcgggaaa ttccgcgctg cgtcaaatct atatctcggt caaccggcgc ctgcaggcat
      601 tgcggttccg ctccaatttc cagaccgcga agtgggatca ggccgtggcc gagcacgaag
      661 agatgctcaa ggcactcgag gcgcgcgacg gtgcgcgcct ggcggcgata ctgcgccagc
      721 atttgctgga gaagcgtgac gcggttctgc aattgctggc cgacgacgca ccccagcact
      781 gatccacgcc atgccgtccc attccaaaga acccatcgcc gctatcgcca cggcccccgg
      841 tcgcggcggc atcggtatcg tgcgcgtgtc cggacccgat ctggacgaac tggtccgcgc
      901 gctgtttgac gacatgctgg cgccgcgcaa ggcccactac cgcccattca acgatcgcca
      961 cggcgaggcc atcgacgccg gcgtggcgct ctacttcaaa agcccgcatt catacaccgg
     1021 cgaagacgtg ctggaactcc agggccatgg cggcaccgcc gtgctgcgtc gcctgctcga
     1081 acgctgcctc gaagccggcg catcactggg catccggcat gcccggcccg gtgaattcac
     1141 tgagcgggcc ttcctgaacg agcgcctgga cctggcccag gccgaggccg tcgccgacct
     1201 gatcgacgcg tcatcgaccg ccgccgcgcg cagcgccatg gcctcactga gcggcgtgtt
     1261 ctccacccgc gtcaacgccc tggccgagcg catcatcaac ctgcgcatgc tggtcgaagc
     1321 caccctggac tttcccgaag aggaaatcga ctttctgcga agctaccagg cacgggagaa
     1381 actcaccgac atccgcagcg acctcgacac catcatccgc cagactcggc aaagcatggc
     1441 cctgcgcgaa ggcctgtacg tcgtcctggc aggccagccc aatgtgggca agtccagcct
     1501 gctcaacgcc ctggccggtg acgacatcgc catcgtcaca cccatcgcgg gcacgacccg
     1561 cgacaaagtc acccagcaga ttcacataga tggtgtgcca atacacattg tggataccgc
     1621 cggactgcgg gaaaccgacg acacggtgga acgcctgggc atggaacgca cctgggcgga
     1681 actggaaaag gccaacgtca tcctacatat gcaggatgtg ggccgtcgca acgatgccct
     1741 ggatacggcc atcgtgcagc ggttgccgaa aggcatcccg gtgttggcgg tgctgaacaa
     1801 aatcgatctg gcacaaaagc cggaaccgcc ggcggcggga tcgaacgaac tccacatctc
     1861 cgcgaaaacc ggtgtcggcc tggatgaact caaacgccgg ctcctcgaag tcgccggtcg
     1921 ggatccggga atggaatccc cctggctggc gcgcgaacgc catctgcttg cgctgaacaa
     1981 cgcgggcgaa catcttgccc tggccgacga acacgctggc cacaacgatc aagtgctgga
     2041 tctgttcgcc gaagagctgc gcctggcgca tggggacctg tgcgccatca ccggccagtt
     2101 caccagtgac gacctgctgg gtgagatttt ctcccggttt tgcatcggaa aataattttt
     2161 gctgagaaat ttaaagatgt gggtgagctg gtctgatatg atccgctcgc cagtccctat
     2221 cacacttcat caccgggatg gataaagcaa cgtctcagaa gacgccggac tcatccggcg
     2281 gttctgtggt ttcgacaaag tgaggatgca ttgggcgcca cagcgatcca catcgaccag
     2341 tttgtgcata tccgcatcat catcggcata ctgctgggtc tgagcatttc ccgcctcgta
     2401 acaggactct cccgatcggt gcaaacgtcg gcgtccttca gaatctaccc cctgcacgtc
     2461 gtctggatcg tctatctatt gctggccatc gttgatttct ggtggttcga gtatgggctt
     2521 ttgaaaatca aggtgtggac cttcgggcta tacctgttcg tgattttcta cgcatcgatc
     2581 ttcgttttca tatcgtccat cctgtttccc gaccgcggtc acggatacgt cagctatgag
     2641 aagtatttcc agggtcgacg ccgcccgttc tattgcatga tgttgctgct gtctgtgctc
     2701 gatgtcgtcg actcgtggat caagggtcgc gaacacttcg cgtcgcttgg cgtggattac
     2761 ctcgtggtgc aggccatcga agcgcttggc gcgctcgttg ccatcattgt ggccagccgg
     2821 aagtatcaaa tggcgtatgc cttgaccgca gtggcgtgcg aggtgtatct gctcagcaag
     2881 atgttctgac cgccgcgtta tgccgtggtg gactggtcag gctttttgac aaaaacgacc
     2941 ttgcaccgct gacgcggcga ctcaggaata ccgtgtcaga cacccgacga acaccatatc
     3001 aagcgcccgg attctcccgc cagtccagtt tccatgcccc tgttcgcaca ttctggagcc
     3061 ataacgcaca ggtcagggtt ttcgcgtcag tgacacgccc ttcccggcat gcctcgagga
     3121 gctcctccac acccatggtg cgcatctcga ggaattcgtc ctgatcgaga tgtcggggcc
     3181 ccgccctcag ccctcgcgcg aagaagatgt ggatgatctc ggtggaatag gcgatggcca
     3241 gatgcatcgc cccggcgctc gcccagtggg tcgcggtgta tccggtctcc tccagcagtt
     3301 cgcgcttggc gcacgccagc ggatcttcgc cagcgtccag cttgccggcc gggaactcgg
     3361 tcatcacgcg ccccaccggg taacggtatt gacgctccac cacgacacgc ccgtcgtcca
     3421 acaacggaat gacgaccacc gcgcccggat gcaccacata ctcgcggctg gcttgcgcgc
     3481 catcgggcag gcgcaccctg tcccgacgaa acgtcaagaa gccaccttca accaaggtct
     3541 gcccatcgat acgggtttcg acaagcgatg cgtcgtcggg gccggtcgtc ataagatgtc
     3601 tctcgatatg tgaacgccag ccagcttacc atttcaacca ggacccaacc ccgctcttga
     3661 agcaaggcag gatcgcggcg tttaattttc ggtgaattcc gccgtaacag cgtcacccct
     3721 cactgacacg gaaaggagaa cggtcatgga catcggacgc atgaaggatc aacacgtgca
     3781 aatactgacc agcatctcgg acctgcgtga gctggcccac gcgggcgttg cgcaaaacgc
     3841 ggcgcacatc gccagggaac tcaaggccat ggggggcgtg atcgtcacac atctggccgc
     3901 ggaagaccgc gtgctgtatc cctctatgaa gaagcagccc aaccaggaac tggttcggct
     3961 ctgccagcag taccaggacg aaatgagcga tctcgcaagc gctttcaaac atttttcaca
     4021 tcactggacg aacgtgtccg aaatcgagcg tcacccggag caattccgcg aagaagccaa
     4081 tgtcgtgatg aaaaaactgc acacgcgcat acgacgggaa aacaccgagc tttatccggt
     4141 cgtggaagcg ctttaggcca ggcatccagg ggatccccgg cggggcacat caacattttc
     4201 cggagtcggg cgccattctc tgttaaaaaa gggtaacccg ttggcatgga acaatccccc
     4261 atgccgcgcc cgccctccag acagaagcgc ccatgtctca cgccgaaccg actctcgtac
     4321 aacacctgcc cgatggcccg ctggacatcg tgggcgacat ccatggcgag ttgtccgcgt
     4381 tgttggccct gctcgtgcgc cttggctaca acagccaggg cggacatccc gagaatcgca
     4441 agctggtgtt cctcggcgat ctgtgcgaca gaggccctga cagcgtcggg gtcatccagg
     4501 tggttcagcg cctcgtcgaa cacggcaacg cccaagcgat cctgggcaac cacgaactca
     4561 atctgctgat aggcgacgcc aaggatggtt caggctggtt tttcgatgag cg
//
LOCUS       DASTNK010000112        10852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_85259, whole genome shotgun sequence.
ACCESSION   DASTNK010000112 DASTNK010000000
VERSION     DASTNK010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10852
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85259"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="VFR20_08285"
     CDS             complement(<1..149)
                     /locus_tag="VFR20_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU0230556.1"
                     /translation="MTSLTTSIPILPGNWLGMMGGGQLGRMFCHAAQSMGYKVAVLDP
                     AGQCPA"
     gene            complement(314..841)
                     /gene="purE"
                     /locus_tag="VFR20_08290"
     CDS             complement(314..841)
                     /gene="purE"
                     /locus_tag="VFR20_08290"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU0230557.1"
                     /translation="MAEHSESNSSGAPLVGIIMGSSSDWDVMKNAAGILEEFGVPHEV
                     RVVSAHRMPLDMVEYGSQALGRGLRAIIAGAGGAAHLPGMMAALTPIPVFGVPVPSRH
                     LQGEDSLLSIVQMPKGIPVATFAIGDAGATNAALHVVACLATTDAALQQKLIEFRERQ
                     TEAARAMVVPPHVRM"
     gene            complement(944..1822)
                     /locus_tag="VFR20_08295"
     CDS             complement(944..1822)
                     /locus_tag="VFR20_08295"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU0230558.1"
                     /translation="MQALLQSTIHSLPLLGRGKVRDMYAVGDDKLLIVATDRISAFDV
                     ILDDPIPGKGAVLTALTEFWLGKLAHIVPNHSTGIAPEEVVAADERDQVMGRAVVVKR
                     LRPILVEAVARGYLAGSGWKDYQAHGSVCGVALPSGLNQAGRLPSPVFTPAAKAEFGT
                     HDENVDFAHVVAEAGRANAEKIRDITLALYSEASAFAASKGIIIADTKFEFGLDGDGG
                     LYLMDEVLTPDSSRFWPASEYREGISPPSFDKQFVRDWLETQPWDKTPPAPRLPADVQ
                     EKTAAKYREALTRLAS"
     gene            complement(1891..2955)
                     /gene="fba"
                     /locus_tag="VFR20_08300"
     CDS             complement(1891..2955)
                     /gene="fba"
                     /locus_tag="VFR20_08300"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395676.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEU0230559.1"
                     /translation="MALVSMRQLLDHAAENGYGIPAFNVNNLEQVQAIMEAAAETDSP
                     VIMQASAGARKYAGEGFLKYLIQAAVESYPSIPVVMHQDHGQSPDVCRGAIGLGFSSV
                     MMDGSLKEDGKTIADYDYNVGVTRRVVDMAHQVGVTVEGELGCLGSLETMKGDKEDGH
                     GAEGTMTREQLLTDPEQAADFVRHTQLDALAIAIGTSHGAYKFTRKPTGDILSIARIK
                     EIHKRLPNTHLVMHGSSSVPQELLAEIRQFGGDMKETYGVPVEEIQEAIKYGVRKVNI
                     DTDIRLAMTGAIRRFMAENPGKFDPREYLKPARQAAKLVCVQRYQQFGTAGNASKIKA
                     VPLTTMAQRYGAGDLAQVVQ"
     gene            complement(3176..3799)
                     /gene="mog"
                     /locus_tag="VFR20_08305"
     CDS             complement(3176..3799)
                     /gene="mog"
                     /locus_tag="VFR20_08305"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HEU0230560.1"
                     /translation="MEQQSRLARSHPDELLVGLVSVSDRASSGVYEDQGIPALEAWLS
                     RALTRAPQIASRLIPDELGVISATLRHLVDVLGCDLVLTTGGTGPARRDVTPEATLAV
                     ATREMPGFGEQMRQISLRFVPTAILSRQVAVIRETADHAALIINLPGQPKAIQETLEG
                     LKDGQGGVLVPGIFAAVPYCIDLIGGPYTETHDEVVKAFRPKSARRG"
     gene            complement(3902..4660)
                     /locus_tag="VFR20_08310"
     CDS             complement(3902..4660)
                     /locus_tag="VFR20_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004543860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0230561.1"
                     /translation="MRLAGKVAIVTGAGSGFGAGIAQTFAREGASVVVADIHEAHGRA
                     VADDIVKAGGKAVFAPVDVADGASVARLLELTLGAFGALHTVVNNAGTTHKNKPLLDI
                     TEQDFDRVFNVNVKSIFWTATHMVPYFRRHGGGSFVNIASTAAIRPRPGLVWYNSTKG
                     AVVTASKAMAAELGKDNIRVNCVNPVIGATALLEEFMGMPDTPENRGKFLAGIPLGRF
                     STPQDVANAALYFASDEAGFITGTCLEVDGGRCV"
     gene            complement(4773..6206)
                     /locus_tag="VFR20_08315"
     CDS             complement(4773..6206)
                     /locus_tag="VFR20_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0230562.1"
                     /translation="MIHKNFIGNKWVSAMSGETVPVIDPSDGQIYAEIARSGQVDVDA
                     AVSAARAAYVGAWGKMAPATRSRLLWRIAAALLDRTDELARIESRDTGKPLKQARADA
                     AAVARYFEFYAGAVDKLHGETIPYPHDFTVLTVREPHGVTGHIIPWNYPLQIFGRSVG
                     ATLAAGNACVVKPAEDACLSLLRVAEIVVEAGLPEGALNICTGLGPEAGAALSAHPGI
                     DHISFTGSPGTGKQVAQAAAAHFAPVTLELGGKSPQIVFSDADIDAALPVLLNAIVQN
                     AGQTCSAGSRLLVQRAIYSDLLSRLAELFKATRTGAAADDLDCGPLINAKQLTRVRGF
                     LEEADESGIAVVAQGALDASAPATGFYQPPMLLADVPPAHRLAQEEVFGPVLAAIPFE
                     TEADALTIANSTPFGLVAGVWTQDGGRQMRMARQVRAGQVFVNNYGAGGGVELPFGGT
                     GQSGFGREKGFEALYGFTTLKTIAIRH"
     gene            6411..7421
                     /locus_tag="VFR20_08320"
     CDS             6411..7421
                     /locus_tag="VFR20_08320"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEU0230563.1"
                     /translation="MADPTPQSSNSDATRTPPSLRVYVACVFPEPVLDLFVQRFGADA
                     NPTGRPLSSTELLAAVQDMDALVVTAPDRLDRAVVNQLPSSVRVIATYSVGTDHIDID
                     AVRSRGIALYSTPDVLTESCADTAILLMLGAARRVIESMDLIRDGTWTGWSPRQLLGQ
                     DFWGRRLGILGMGRIGRAVAARARGFGMRVHYHNRHRLEPGLEADAVFHEHLDDLLEA
                     SDFLCVACPSGPGTRGLLDAAAIARLPHGAIVANIARGDIIDDAALVRALETGAVAAA
                     GLDVFAGEPDIHPAYRTMPNVFGLPHIGSSTMATRLAMGRLLCDSLEAFQMGRSTSNR
                     LC"
     gene            7432..8313
                     /locus_tag="VFR20_08325"
     CDS             7432..8313
                     /locus_tag="VFR20_08325"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0230564.1"
                     /translation="MAKTNQPGDTPASPARNVLLAGAGDVCLRIARRLAAQGDQVWGL
                     RRQPPAGDTDGIRWIAADLTRPNTLTVIPSAISHVIYAPSPDARDESAYRHVFVNGLD
                     NLFDQLDHATLQRVVFLSSSAVYGDQGDEWVDEDTPVQPKGFNGRILVEAEKRLAAIQ
                     SDVVVLRLAGLYGPGRLQLLERLKNGQARVHTSGRQWANRLHVDDAARATVHVLGLQA
                     PTPCYVVADDTPHHIGELYDQLAAMIGAPPVPRDERLAANTGRRLRNARLKASGFTPL
                     WPDAIAGYRALLGEFRE"
     gene            complement(8445..9641)
                     /locus_tag="VFR20_08330"
     CDS             complement(8445..9641)
                     /locus_tag="VFR20_08330"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU0230565.1"
                     /translation="MTAIKTLSGLAREGALAGKRVFIRSDLNVPFDDTGRISEDTRVR
                     ASVPAIRMALDAGAAVMVSSHLGRPKEGVMGAGDSLAPVAARLSELLGREVRLVSGWV
                     DGVEVEPGQVALLENARCNVGEKKNDESLARRMAALCDVYVNDAFGTAHRAEATTHGI
                     ARFAPVACAGPLLEAELAALGRALRDPARPMVAIVGGSKVSTKLSILQSLASKVDHLI
                     VGGGIANTFMLAAGLGIGQSLAEPGQVEEARAVMDIMARRGSQVPVPTDVVCAKSFSP
                     DALATVKPAGEVAADDLILDIGPETAARLAGILRGAGTIVWNGPVGVFEFDAFANGTR
                     VIAEAVAQSPAFSIAGGGDTLAAIAKYGIADKVGYISTGGGAFLEFLEGKTLPAVEVL
                     AQRAGA"
     gene            complement(9709..10719)
                     /gene="gap"
                     /locus_tag="VFR20_08335"
     CDS             complement(9709..10719)
                     /gene="gap"
                     /locus_tag="VFR20_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU0230566.1"
                     /translation="MTIRVAINGYGRIGRNILRAHYEYGKKHDIEIVAINDLGNPETN
                     AHLTRFDTAHGRFPGTVSVEGDCLVVNGDKIRVLATRDPSTLPWKELGVDVVLECTGL
                     FTSKEKASAHLKGGARKVIISAPGGKDVDATIVYGVNHGVLKASDTVISNASCTTNCL
                     APLVHPLHKELGLVSGLMTTVHAYTNDQVLTDVYHSDLRRARSATHSMIPTKTGAAAA
                     VGLVLPELNGKLDGYSIRVPTINVSIVDLSFVASRETSVEEVNGILKKASEGALKGIL
                     QYSTDPLVSVDYNHDPASSTVDAALTKVSGTLVKVASWYDNEWGFSNRMLDTTVALMA
                     AK"
BASE COUNT         1961 a         3638 c         3378 g         1875 t
ORIGIN      
        1 gccggacatt gcccggccgg atccagcacg gccaccttgt accccatcga ctgcgcggca
       61 tgacagaaca tgcggcccag ttgtcctccg cccatcatgc ctagccagtt cccaggaagg
      121 ataggaatcg atgtggtgag cgacgtcatg ggcaagggaa tccgaaaaaa gaagaaagcg
      181 acaaataaaa cacgaaaggt aaagcgctgg gcaacgactt gggccagcga gccaatgtct
      241 tggagattat cgctttggca gcaaggtcac cgttagtctc ggccggacca acgccaaagc
      301 caaaagcgaa acgctacata cgcacatgcg gaggcaccac catggcccgc gccgcctcgg
      361 tctggcgttc gcgaaactcg atcagctttt gctgaagcgc ggcgtcggtg gtggccaggc
      421 aggccacgac atgcagcgcc gcattggttg cgcccgcgtc gccgatggcg aacgtggcca
      481 cggggatgcc cttcggcatc tgcacgatgg acagcaacga atcctcgccc tgcagatgac
      541 gcgagggcac gggaacgccg aatacgggaa tgggcgtgag cgccgccatc atgcccggca
      601 ggtgcgccgc accacccgcg cccgcgatga tggcccgcag cccgcgaccc agcgcctgcg
      661 agccatactc gaccatgtcc aggggcatgc gatgcgccga caccacccgc acctcgtgcg
      721 ggacaccaaa ctcttccagg atgcccgccg cgttcttcat gacgtcccag tcgctggacg
      781 accccatgat gatgcccacc agcggcgcgc cggaggaatt cgattcgcta tgttcggcca
      841 tgaaacacgc cctcgccaag aagtgatggt gaaacaatca ttttaacgcc tgcaaaatgc
      901 gaacggccgc gcaggcggcc gttcgtggca ggatcatgac cggtcaggaa gcgagccgtg
      961 tcagggcttc gcggtattta gccgcggtct tttcctggac gtcggcgggc agcctgggcg
     1021 cgggaggggt cttgtcccag ggctgggttt caagccagtc gcgcacgaac tgcttgtcga
     1081 acgacggcgg gctgatgcct tcgcggtact ccgacgcggg ccagaaacgc gacgaatcgg
     1141 gcgtgaggac ctcgtccatg agataaagcc cgccgtcacc gtcaagcccg aactcgaact
     1201 tcgtgtcggc gatgatgatg cccttggaag cggcgaaggc cgatgcctcg gaatacaacg
     1261 cgagcgtgat gtcgcggatc ttttccgcgt tcgcgcgccc cgcctcggcc accacgtgcg
     1321 cgaaatcgac gttctcgtcg tgggtgccga attcggcctt ggccgccggc gtgaacacgg
     1381 gcgaaggcag ccgacccgcc tgattcaggc ccgacggcaa cgccacacca cagaccgacc
     1441 cgtgcgcctg ataatccttc cagcccgacc cggccagata accacgggcc accgcctcga
     1501 ccagaatggg cctcagacgc ttgaccacga ccgcgcggcc catgacttga tcacgctcgt
     1561 cggccgccac cacttcttca ggcgcgatgc ccgtggaatg gttgggcacg atgtgcgcga
     1621 gcttcccgag ccagaattca gtcaatgccg tgagcacggc acccttgccc gggatagggt
     1681 cgtcgagaat gacgtcaaag gcggagatgc gatcggtggc gacgatgagc agcttgtcat
     1741 cacccacggc atacatgtcg cggaccttgc cgcgccccag caagggaagg gaatgaatcg
     1801 ttgattggag tagagcctgc accgggaatg tcctgaaacc tgaaaccgaa agacagcgcg
     1861 ccggcttccc ggcgcgcttc aagtctgtga ttattgcacc acctgtgcga gatcgccggc
     1921 gccatagcgc tgcgccatgg tcgtcaacgg caccgctttg atcttgctgg cgttgcccgc
     1981 tgtgccgaac tgctggtaac gctgaacgca gaccagcttg gccgcctggc gcgcgggctt
     2041 gaggtattcg cgcgggtcga acttgccggg gttctcggcc atgaaacggc ggatggcgcc
     2101 cgtcatggcg aggcggatgt cggtgtcgat gttgaccttg cgtaccccgt acttgatggc
     2161 ttcctggatt tcctccacag gcacgccata ggtttccttc atgtcgccac cgaactggcg
     2221 gatctcggcc aggagttcct gcggaacact ggagctgcca tgcatgacca gatgcgtgtt
     2281 gggcaggcgc ttgtggattt cctttatgcg ggcgatcgac aggatatcgc ccgtgggctt
     2341 gcgggtgaac ttgtaggcgc cgtggctggt gccgatggcg atggccagtg cgtcgagctg
     2401 tgtgtggcgg acgaaatcgg cggcctgttc cgggtcggtg agcagctgct cgcgcgtcat
     2461 ggtgccttcg gcgccgtgac cgtcttcctt gtcacccttc atggtttcca gcgaccccag
     2521 gcaacccagt tcgccttcga ccgtgacacc cacctggtgc gccatgtcga ccacgcgccg
     2581 cgtgacgccc acgttgtagt cgtagtcggc gatggtcttg ccgtcttcct tcagggagcc
     2641 gtccatcatg acgctggaga accccaggcc gatggcgccg cggcagacgt cgggcgactg
     2701 gccgtgatct tgatgcatga ccaccggaat cgacgggtag gattccacgg cggcctggat
     2761 gagatatttc aggaagccct cgcccgcata cttgcgcgca ccagccgacg cctgcatgat
     2821 gaccgggctg tcggtttctg ccgcggcctc catgatggcc tggacctgct cgaggttgtt
     2881 gacattgaac gccggaatac catagccatt ctcggcggca tgatccagca actggcgcat
     2941 ggacactagg gccataacac gatctcctga aagcgaataa acgggaatcg cgtcatttta
     3001 cgaggtaacg ccgcaagttg cttgcacggg cgcgcacacg ggtaactctt tgcaatcacc
     3061 gtcagggacg gcgaccggca tcggacgatc ccttgcgagg agcttcccca gggtcacgtg
     3121 tgtagagccc gaggatcacc gcctgcttgc acaccggggc ccctgcgccc gccaatcagc
     3181 cgcgccgcgc cgacttgggc cggaacgcct tgacgacctc gtcgtgggtt tccgtatacg
     3241 gcccgccgat caggtcgatg caatatggca ccgcggcgaa tatccccggc accaacaccc
     3301 cgccctggcc gtccttgagc ccttccagcg tttcctggat ggccttgggt tggcccggca
     3361 gattgatgat gagggcggcg tgatcggcgg tttcacggat cacggccact tgccgggaca
     3421 ggatcgccgt cggcacgaac cgcaggctta tctggcgcat ctgctcgccg aagccgggca
     3481 tttccctggt ggcgacggcc agcgtggctt ccggcgtgac gtcgcgccgc gccggacccg
     3541 tgccgccggt cgtcagcacc aggtcacagc ccaggacatc gaccagatgc ctgagcgtgg
     3601 ccgaaatcac cccgagctcg tcgggaatca accgtgaggc gatctgtggt gcccgcgtga
     3661 gggcgcgtga cagccaggcc tccagggccg ggataccctg gtcttcatat acgccggacg
     3721 aggcgcggtc ggacaccgac accagcccca ccagcagttc atcggggtgg ctacgagcga
     3781 gccgggattg ctgttccatg aaaaattcct gatgatgcga acgacgttcg caacgcgaaa
     3841 atccgagagc caccaccatg cggcggtgtg aaacgcagac ccgcggctta cgctttcaag
     3901 ttcaaacgca acgcccgcca tccacctcga ggcacgtgcc cgtgatgaaa ccggcctcgt
     3961 cggacgcgaa gtacagcgcg gcgttggcca cgtcctgggg cgtggagaac cggcccaacg
     4021 gaatgcccgc caggaactta ccgcggttct ccggcgtgtc gggcatgccc atgaactctt
     4081 ccagcagggc cgtcgcgcca atgaccgggt tcacgcaatt gacccggata ttgtctttgc
     4141 caagctcggc cgccatggcc ttgctggccg tgaccacggc gcccttggtg ctgttgtacc
     4201 agaccagccc ggggcgcggc cggatcgcgg cggtggaggc gatgttgacg aacgaaccac
     4261 cgccgtgccg ccggaagtac ggcaccatgt gcgtcgccgt ccagaagatg ctcttcacgt
     4321 tgacgttgaa cacccggtca aagtcctgct cggtgatgtc cagcaatggc ttgttcttgt
     4381 gcgtggtgcc ggcgttgttc acaaccgtgt gcagcgcgcc gaaggcaccc agcgtcagct
     4441 cgagcaggcg cgccacactg gctccgtcgg ccacgtccac cggcgcgaac accgccttgc
     4501 cccccgcttt cacgatgtca tccgccacgg cgcggccatg cgcctcgtgg atgtcggcca
     4561 cgaccacact ggccccttcg cgcgcgaacg tttgcgcaat gccggccccg aaacccgagc
     4621 ccgcccctgt cacgattgcc accttgcccg ccagacgcat gattagtcct gtgaaaatga
     4681 ttggttgatg aagaaacaaa accccatgcc gtctcgctct cagcggttcg ccataggcga
     4741 aaagccctcg cctgaggaag ccggtccggt atctagtgtc gaatcgcgat ggtcttgagc
     4801 gtggtgaaac cgtacagcgc ctcgaacccc ttctcgcgcc cgaagccgga ctggcctgta
     4861 ccgccgaacg gcagttcgac accgccaccc gcgccatagt tgttgacgaa cacctgcccg
     4921 gcacgcacct ggcgcgccat gcgcatctgc cgcccgccgt cctgcgtcca gacccccgcc
     4981 accaggccga acggcgtgga attggcgatg gtcagcgcat cggcctcggt ctcgaacggg
     5041 atggcggcga gaaccgggcc aaagacctct tcctgggcca ggcggtgcgc cggcggcacg
     5101 tcggcaagca gcatcggtgg ctggtagaaa cccgtggctg gcgccgacgc gtccagagcg
     5161 ccttgtgcca cgacggcgat gcccgattcg tccgcctctt ccaggaagcc gcgtacccgc
     5221 gtcaactgct tcgcgttgat gaggggccca cagtccagat cgtcagccgc ggcgccagta
     5281 cgcgtcgcct tgaacagctc ggccaagcgg ctcaacaggt cggagtagat ggcacgctgc
     5341 accagcagac ggctgcccgc ggagcacgtc tgccccgcgt tctggacaat ggcgttcagg
     5401 agcacgggca gcgcggcgtc gatgtcggcg tcggagaaga cgatttgcgg cgactttccg
     5461 cccagctcca gcgtgacagg agcgaaatgc gcggcggcgg cctgggccac ctgcttgccg
     5521 gtgcccggcg agccggtgaa cgatatatgg tcgatgccgg ggtgcgcgga caacgccgcg
     5581 cccgcctcgg gccccaaccc ggtgcagata ttcagcgccc cttccggcaa gcccgcctcg
     5641 acgacgatct cggcgacgcg aagcagcgac aggcaggcgt cctcggccgg cttgaccacg
     5701 caggcgttgc ccgcggccag cgtggcgccc acgctgcgcc caaagatctg caacggataa
     5761 ttccagggaa tgatgtggcc ggtcaccccg tgcggttcac gcaccgtcag taccgtgaaa
     5821 tcgtgcggat aaggaatggt ttcgccgtgc agtttgtcga ccgcccccgc gtagaactcg
     5881 aaataccgcg ccaccgccgc cgcatcggcg cgggcctgct tcaacggctt gcctgtatcg
     5941 cgcgattcga tgcgggcgag ttcgtctgtg cgatcgagca gcgcggcggc gatgcgccac
     6001 aacagtcggc tgcgcgtggc gggcgccatc ttgccccagg cccccacgta cgcggcacgc
     6061 gccgccgaca ccgccgcgtc gacgtcgacc tggccggagc gcgcgatttc agcatagatt
     6121 tggccatcgg acggatcgat gacgggaacg gtctcgcccg acatcgcgct gacccacttg
     6181 ttgccgatga aattcttgtg aatcatggtc tcatcctcga gcgttggcag cggaggcatg
     6241 gatctgcccc agcagaaacg aatccccagt ttatcagtgt ccacccgtcg ccgctgcgcg
     6301 tcctggaaca tggccgatgg cggtcgcgcg cggcgcggtc cgccgtttcg tggcaccgcg
     6361 ttgccctaca atcggactac ccgccacgac ctcccgaaag gacgacagcc atggctgacc
     6421 cgaccccgca atcctccaat tccgacgcca cacggactcc tccctcgctg cgtgtctatg
     6481 tcgcctgtgt cttcccggaa cccgttctgg acttgttcgt acagcggttc ggtgccgacg
     6541 cgaacccaac cgggcgacca ctgtcatcga ccgaactgct cgcggctgtc caagacatgg
     6601 atgcgttggt cgtcaccgcg cccgatcgtc tggaccgggc ggttgtcaat cagctgccat
     6661 cctccgtgcg cgtcatcgcc acgtattccg tcggcaccga ccacatcgat atcgacgccg
     6721 tccgctcacg cggcatcgcc ctgtacagca caccggacgt tctcaccgaa tcgtgcgccg
     6781 ataccgcgat tctgctgatg ctgggcgccg cgcggcgcgt gatcgaaagc atggacctga
     6841 tccgcgatgg cacctggacc ggctggtcac cccgtcagct cttgggtcag gatttctggg
     6901 gcaggcgcct gggcatcctg ggcatgggac gcatcggccg cgccgtggcg gcgcgggccc
     6961 gtgggttcgg catgcgggtc cattatcaca atcgccaccg gctggagccc ggcctggagg
     7021 ccgacgcggt cttccacgag cacctggacg acctgctgga agccagcgat ttcctctgtg
     7081 tcgcgtgccc ttccggaccc ggcacgcggg ggttgctcga cgcggcggcg atcgcgcgtc
     7141 tgccccatgg cgcgatcgtc gccaacatcg cacgcggcga catcatcgac gatgccgcgc
     7201 tggtgcgcgc cctggaaacc ggcgcggtcg cggcggcggg cctggacgtc ttcgccggcg
     7261 aaccagatat ccacccggcc taccgcacta tgcccaatgt gtttggcctg ccccacatcg
     7321 gcagttcgac catggcgacc cggctcgcca tgggccggct gctgtgcgac tcgctggagg
     7381 cattccagat gggccgttca acctcgaatc gcctgtgcta gggtgccgcg tttggccaaa
     7441 acgaatcaac ccggcgacac accggcatca ccagccagga atgtcctgct ggccggcgcc
     7501 ggtgatgtct gcctgcgcat cgcgcgacga ctcgcggcgc agggcgatca ggtatggggg
     7561 ctgcgccgac agcctcccgc tggcgacacc gacgggatcc gctggatcgc cgccgacctc
     7621 acccgaccca acacgctgac cgtgattccc tccgcgatca gccacgtgat ctatgccccc
     7681 tcccccgacg cgcgcgacga atccgcctac cgccatgtgt tcgtcaacgg gctggacaat
     7741 ctattcgacc aattggatca cgccacgctg caacgagtcg tttttctgtc ttccagcgcg
     7801 gtctatggcg accagggcga tgaatgggtg gatgaagaca ctcccgtgca accaaaaggt
     7861 ttcaacggcc ggatcctggt cgaagccgaa aagaggctgg ccgcgatcca gtccgacgtc
     7921 gtcgtactga gactcgccgg cctgtacggc cctgggcggc tgcaattgct ggaacgcctg
     7981 aagaatggtc aggcacgggt gcatacgtca ggccggcaat gggccaatcg cctccatgtc
     8041 gacgacgccg cccgggccac cgtgcatgtg ctggggcttc aagccccgac gccctgctac
     8101 gtcgtcgccg atgatacgcc gcatcacatt ggcgagctgt atgaccagtt ggcggcgatg
     8161 atcggcgcgc cacctgtgcc acgcgatgag aggctcgccg ccaacaccgg caggcgcctg
     8221 cgcaacgccc ggctgaaagc cagtggcttc acaccgcttt ggccggatgc catcgcgggc
     8281 taccgggctt tgctcggtga attcagggag tagcggcaag ggcgccagcc caaagtgccg
     8341 tttggttcaa gcattcaacg aacaaccaaa cagcgcactg gacatcgcac acccgaaggg
     8401 tgacgggccg agttgtggat ggtcgttgct cagacgacgc ccaatcacgc gcccgcgcgc
     8461 tgggccaata cctcgaccgc cggcaacgtc ttaccttcca ggaactccag gaaggccccg
     8521 ccgcccgtcg aaatataacc caccttgtcg gcaatgccgt acttggcgat ggccgccagc
     8581 gtgtcgccgc cgcccgcgat cgagaaggcc ggggattgcg ccacagcctc ggcgatgaca
     8641 cgcgtaccgt tggcgaaagc gtcgaattcg aagacgccca ccggtccatt ccagactatg
     8701 gtgcccgccc ctcgcagaat acccgccaat ctggcggccg tctctggccc gatgtccagg
     8761 atcaggtcat cggccgccac ttcccccgca ggcttcaccg tggccagcgc atcgggggaa
     8821 aatgatttgg cgcacacgac atcggtcgga accggcacct gggacccgcg ccgcgccatg
     8881 atgtccatga ccgcgcgcgc ctcttcgacc tggccgggct cggccagtga ctggccaatg
     8941 cccagccccg ccgccagcat gaaggtgttg gcgatgccgc cgcccacgat gaggtgatcg
     9001 accttggacg ccagcgactg gaggatggac agcttggtgg acaccttgga accacccacg
     9061 atggccacca tgggccgggc cgggtcccgc aaggcgcggc ccaatgccgc cagttcggcc
     9121 tccagcaacg ggcccgcaca ggccacaggc gcgaatcgcg cgatgccgtg cgtggtcgcc
     9181 tcggcccgat gcgcggtgcc gaacgcatca ttcacataca catcgcatag cgcggccatc
     9241 ctgcgtgcca gggattcatc gttcttcttc tcgccaacgt tgcagcgcgc gttttcgagc
     9301 agggccacct gccccggttc aacctcgaca ccgtccaccc agccgctcac cagccgcacc
     9361 tcgcgaccca gcagttccga cagccgcgcg gcgaccggcg ccagcgagtc gccggccccc
     9421 atcacgccct ctttcggccg ccccaggtgg gaactgacca tcaccgcggc gccggcatcc
     9481 aacgccatgc ggatggccgg aacggaggcg cgaacccggg tgtcttcgct gatgcggccg
     9541 gtgtcatcga acggaacgtt cagatcggat cgaatgaaca cccgctttcc ggcaagcgcg
     9601 ccctcgcgcg ccaggccgga cagagtcttg attgcagtca tgagattttc caacgcacaa
     9661 atgcggcgag ggcgggaatc atcccgccct cgccatcgtc aaagagtttt attttgccgc
     9721 catcaaggcg acggtggtat cgagcatgcg attggagaag ccccattcgt tgtcgtacca
     9781 ggacgcgacc ttgaccagcg tgcccgacac cttggtcagg gcggcatcaa cagtgctgga
     9841 cgccggatcg tggttgtaat ccacggagac caggggatcg gtgctgtact gcaggatgcc
     9901 cttcagggcc ccttccgacg cctttttcag gatgccgttg acttcctcga ccgaggtctc
     9961 gcgcgaggcc acgaacgaca ggtcgacgat ggacacattg atcgtcggga cgcggatgga
    10021 atagccatcg agcttgccgt tcagttcagg caggaccaga cccaccgccg cagcggcgcc
    10081 ggtcttggtg ggaatcatgc tgtgtgtggc cgaacgggcg cgacgcagat cggaatggta
    10141 gacgtcggtg agcacctgat cgttggtgta ggcgtgcacc gtcgtcatca agcccgacac
    10201 caggcccagt tccttgtgca acggatgcac cagcggagcc aggcagttgg tggtgcagga
    10261 cgcgttcgag atcaccgtat cggaagcctt gagaacgcca tgattgaccc cgtacacgat
    10321 ggtcgcatcc acatccttgc ctcccggcgc ggaaatgatc accttccggg cgccgccctt
    10381 caggtgcgcg ctggctttct ccttggaggt gaacaagccg gtgcattcca gcacgacatc
    10441 gacgccaagc tctttccagg gcagggtcga cgggtcgcgg gtggccagca cacggatctt
    10501 gtcgccattg acgaccaggc agtcgccctc gaccgacacc gtgccgggga aacggccgtg
    10561 ggccgtgtcg aagcgagtca ggtgggcgtt ggtttccggg ttgcccaggt cgttgatggc
    10621 cacgatctcg atatcgtgct tcttgccata ttcgtagtgg gccctgagga tattgcggcc
    10681 gatacggcca tatccgttga tcgctacgcg aatcgtcatt gcttctctcc tagttcaaaa
    10741 cctgttcgac ggccgctgtc acatgctcga cagtcaggcc gaaatgcttg aacaacacgc
    10801 ctgccggcgc ggattcccca taagtatcga taccgaccac ggcgccatcc ag
//
LOCUS       DASTNK010000113        11362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1009953, whole genome shotgun sequence.
ACCESSION   DASTNK010000113 DASTNK010000000
VERSION     DASTNK010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11362
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009953"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VFR20_08340"
     CDS             <1..299
                     /locus_tag="VFR20_08340"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0230567.1"
                     /translation="GQLQRGDTVPEFEKAIFEDATIGVLPKLVATRYGFHIIAVDKRV
                     EGTQLPFDLVREKIAETLRRNSEEKALSQYVRVLAGNADIQGVDLEASASPLVQ"
     gene            637..1971
                     /locus_tag="VFR20_08345"
     CDS             637..1971
                     /locus_tag="VFR20_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0230568.1"
                     /translation="MNIQVNGCRGQARRLLAGVSFVSFASLAGMSCAHAATTDVNVWY
                     AMNAHNSEAFQSLAKEFNRSQDSVKVVLKAFDSPEAVESALQASQGKKGASPNLAQMD
                     DIHSADDMRTRRYILPLHDLLSKHPIKDAKWFLSSSDAFAHDTRGRLTGFPYMASVPV
                     MFYDIDAFHKAKLSPAVPQGTWLGLQAQLVSLANGGSRKCPFAMAVPVSVDLENLAAV
                     NQQFYASGDNGFKAKGMPKFNFDLMYVRHLSLMKSWVSVGIMTRPEAAPQAVSNFASR
                     ECAVLMADSANIGTFNDARSLKYGVSGLPHYPEATKTPGHPFVGGSALWAIKGHPAAQ
                     DKATAQFLAWLAKPANAAKWYQETGYLPLTTQAFDATGAGYYKNLGDWRAIVADYATR
                     PDATERGFWVNNYPQIRVMLKQTLDRALNGQEAAVPALQTATSQADRMVRER"
     gene            2914..3279
                     /locus_tag="VFR20_08350"
     CDS             2914..3279
                     /locus_tag="VFR20_08350"
                     /inference="COORDINATES: protein motif:HMM:NF022136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin co-regulated pilus biosynthesis Q family
                     protein"
                     /protein_id="HEU0230569.1"
                     /translation="MAVPSPSPGTAQPSGAKIDAPITREYFEVGPADGNIRQTLNRWA
                     QVAGWTFNVEHWAVNVDVPIVGSATFDSGFKPAVRALLASTELGDRPLRPCFYSNKVV
                     RVVPYAQVCDRTRNPGSSS"
     gene            3276..4880
                     /locus_tag="VFR20_08355"
     CDS             3276..4880
                     /locus_tag="VFR20_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230570.1"
                     /translation="MSRPRRLVLVVASCMVFTACSLAEKVLEVRKSADRAQAKIGAEH
                     RAFDRAVAGEGERQAAQEVDRPWIAGRPVPLGRDVTLPSALRANVRTTMLFAGGPVDL
                     RQAAGRITAATSIPVFVRPDALLPLERFLPRLSGEVSTPAASGGVSAEVMMGLPAREA
                     EPLADILDRIGARLGVQWRYTAGRIEFFRTETRVFDVRALALNASAQASLGLEGRGKQ
                     QGFVSSSRTRLEGGEQNPMKAIHARLEPFLTRAGILVAQPGAGASVVVTDTPDVLRRI
                     ATYLDAENRALTRRIRLVFEEITLATDDSAEAGIDWNVVFTDARLAASLSIAAPVAAE
                     AARMALGATHGNFQGSEAVIRALSKVGRVVRRSSVPVLTLNRRPVTHAVRTTFSYIDQ
                     VQTTALANGSGLALPSVSVSQRDETVGSVLTLVPDAQDNNQILLSVAYDNTVAQPLKS
                     VTFGDRSNPLQLQQITIDGNGTVQQVLLHPGQPLVISGFDRKQEQSEGRRLNPGLPAI
                     LGGSDQASKQRLMTVIIMSAQVEEDA"
     gene            4894..6363
                     /locus_tag="VFR20_08360"
     CDS             4894..6363
                     /locus_tag="VFR20_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230571.1"
                     /translation="MAPYLILPSSDSALVFGMEWLPLIGRRLESAAYRTARQYKASHL
                     VFSNTGATAVGLADLPTGMPRAGGALRSAAQCLACLFSSGTVALMLEVESGRFWVAAV
                     HDGAVVTGTDRLYPGEDVARAAMEDLKQAYPRLAVLGAPGSPPAPSLRALDSADGTRT
                     RLVRARSGGGIRRTLICSSLAMLMVAPLIIWGVRPRNVALAPTALPAAHQSPATIWRR
                     AVDSARQGHVVHGYAGTRLVLDSFYELPGSMAGWSLTQAACSPEGSGWRCDAMYRRRA
                     NGAGSDVFLDAAPSAWRVSFAGLDQARVSWPLSGTAVALVRHDPHSVRHDDRHLLSQL
                     QALSPAFTRLELGQPVALRITPPRDRGGRVPPRPPGMPRYVTRSVRIMGPLRSAGMLA
                     PLMPSIEWSRVTLASSDVRAPSLTASRFVISFEGVLHESEPLDAEGVSSGRVSTVGQE
                     DKSPQCMHDRCHGAGPAGASGVVGDAGSGQHRGGRDVGA"
     gene            6350..6703
                     /locus_tag="VFR20_08365"
     CDS             6350..6703
                     /locus_tag="VFR20_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230572.1"
                     /translation="MLVRELMRLETRLALRQARQRAQAAETHGTPTSAATSSDLDLEL
                     QAIYGVGNKLLAQVRHNERTLIYLRGSALPVGVKDAAGNQAFRLVGLSGTCVQLAREA
                     VSHTLCLTGSGSAGR"
     gene            6706..8436
                     /locus_tag="VFR20_08370"
     CDS             6706..8436
                     /locus_tag="VFR20_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEU0230573.1"
                     /translation="MWPIRTASTLRSPSAFEPMSARFDAATAVALAGADDLLSLEPAF
                     LAPMHDEFGVEALAAKVCPVLLADHSVAIFAVTGHEGSDQADALAQRARQHGYRLAEP
                     QRYVVAAPLLLAIVRRQFAVPSTRPVGGHMPEASRTALAEAFQDMIEWGVRHGASDMH
                     LNVDLDEPESEVRYTVGGRYVAPECFRRIPTSTLMDILAVAWMDIRGGNGAVFDPTME
                     QQGRLTRRVDGRAVVLRWASLAADRGPSVCLRLLEREPAISSLSLEALGYSVMQIAVM
                     ERAVQAEGGAIVFSGTVGSGKSTTLASLITSLPESRKVVTIEDPVEYVIPRAIQNTVA
                     RDLLAEDNSVYMAKLMAIKRSAMTDVLLGEIRDQQTGRAFMDLAGSGVNLYTTVHAPS
                     AALTGERLASDFIGVSRDFLATPGVLKLIVHQSLLSRLCGHCSLPATSPAECPQGSKP
                     SSWRQWLARIEKLYERSADSLRVRNEKGCAHCLNPQAPALAGYAGRTVVAECIEPRRH
                     PDFLRTLRDGCPGGDLRNTPSSSESSALYDAMSKAFQGDIDPRDIESRFPPIEAQGHH
                     PRAFTKAGPG"
     gene            8436..9566
                     /locus_tag="VFR20_08375"
     CDS             8436..9566
                     /locus_tag="VFR20_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230574.1"
                     /translation="MRFAPFLLVRDVGVRLRDEMDRRRFDRARADYYEYLAAVVGATQ
                     GRRTVKEIFNADALRYGPDTVRGRLSRRWLSAYEASGGDLYSTWRGCFPLSELNLVRA
                     AQHLGNDSLVRTLGDLAAALRLSRQAANILVSTLWSAGVALIVLAGMSFAVPAFTIPS
                     LRQTFHVLTPDYYGAMTTALFGLSDSIRDKWVLVAGLMAGGFCAILWSLPNLCGPARV
                     MAEKLTLWRIYRHIHALWFFSALGIVLGKDGASPTRLRTALWMQKAGASPWRNGHIDA
                     MLARIDAGLVGAATFDTGLLDRDLYWFLCDTELARGLAVGLVMTRQRLELRVLREVAA
                     RAARLRWCLLLSAVAGLVGLGVWHYAVIDELRRSLLIFYASQ"
     gene            9622..10200
                     /locus_tag="VFR20_08380"
     CDS             9622..10200
                     /locus_tag="VFR20_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HEU0230575.1"
                     /translation="MTIAAFRFRHTARQRGFSLIEVSIVTAIVLLLAIIGIPAIGGYV
                     VENKVPKVGEELARFILQVKVNASVGAGQPYADIDTAHFANLVRDSGIFSVTGVGAGA
                     QVLHGLGNDGRVTVAPQGAGESFSLELTNVNHAACPALASVMQRVVDTISVAGEGQAG
                     ATVKDAATAYSALATEAQCVQGDRNTFVFVAS"
     gene            10214..11242
                     /gene="pilV"
                     /locus_tag="VFR20_08385"
     CDS             10214..11242
                     /gene="pilV"
                     /locus_tag="VFR20_08385"
                     /inference="COORDINATES: protein motif:HMM:NF016785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HEU0230576.1"
                     /translation="MTRQRGFALLELVVAALITTLLAVWGANAWVHRLDEANAHAMAA
                     WMLAAREGVQAYIHRHGISMAQMDAGGTESVPGFQDWRTPTVPELKGAGLLADGFPEA
                     GPGGLRVAVRILSGKNCPGSDCSLEALILGSPPLLDKRSGEVDEQKVALWLLASRGLG
                     GVVNPRDPASVRGAAFGFANPPAAGMSMLPVGTPAMAITTAGSSDGAQFLRVRDDRDP
                     DFQGTASVRGGIRSDGSVTAGEYVIVGARHTVRSSCGQEGAVARDAYRGLLICQSGLW
                     RSAGGSGGGGFSTNTVYGCFTSSGLSTANPVTGSCNCPLGHAMVAISDSGSAASPDGR
                     TRGYLCVE"
BASE COUNT         1877 a         3360 c         3800 g         2325 t
ORIGIN      
        1 tggggcaatt gcagcgcggc gatacggtgc cggaattcga gaaggccatc ttcgaagacg
       61 ccacgatcgg ggtgttgccc aagctcgtgg ccacgcgtta cggctttcac atcatcgccg
      121 tcgacaagcg cgtcgagggc acccaactgc cgtttgatct ggtgcgcgaa aaaatagctg
      181 aaaccttgcg tcggaacagc gaggaaaagg ccttgtcgca gtatgtgcgg gtgctggccg
      241 gtaacgccga catccagggg gtcgatctcg aggcctccgc ctcgcctctg gtgcagtagg
      301 gctggaaacc gtctgaacga gccaccgcgt cgcacacctc cgcggtgtac ggccgtccgc
      361 cgcggagttt ggtgcgcgat tgtgcgccgg cgcgtggacc gacgccattt ggtaaccaat
      421 taagacgccc gctccgacga atggcgatat tgcgccaacg gggtgccgca agcactacaa
      481 tccgttgcga tcgatccgga gtttcgttca tgcgggtcgc tcgtcggggg gcggattttg
      541 ctacgcttgc gtacccgcca aggcgtagcg catcgtgggc gggcatctca tccgtgttcg
      601 cggatgtttt caatattttt ccaggctggt cgcactatga atattcaggt caatgggtgt
      661 cgcgggcagg cgcgtcgtct gcttgcgggg gtttcgttcg tctcgttcgc ctcgctggca
      721 ggcatgtcgt gcgcgcacgc cgccacgacc gacgtcaatg tctggtacgc catgaacgcc
      781 cacaacagcg aggcgttcca gagcctggcc aaggaattca accgcagcca ggactcggtg
      841 aaggtggtgc tcaaggcgtt cgacagcccc gaggccgtgg aatcggcgct gcaggccagc
      901 cagggcaaga agggcgccag cccgaacctg gcccagatgg acgacatcca ttcggcggac
      961 gacatgcgca cgcgccggta cattctgcct ttgcacgatc tgctgtccaa gcaccccatc
     1021 aaggatgcga agtggtttct ttcgagttcc gacgcgttcg cgcacgacac gcgcgggcgc
     1081 ctgacgggtt tcccctacat ggccagtgtg ccggtgatgt tctacgacat cgacgcgttc
     1141 cacaaggcca agttgtcgcc ggccgtgccg cagggcacct ggctgggcct gcaggcacaa
     1201 ttggtgagcc tggccaatgg cggttcacgg aaatgcccgt tcgccatggc cgtgccggtc
     1261 tcggtcgatc ttgaaaacct ggcggccgtg aaccagcagt tctatgcctc cggcgacaac
     1321 ggcttcaagg ccaagggcat gccgaagttc aatttcgacc tcatgtacgt gcggcacctg
     1381 tccctgatga aaagctgggt gagcgtcggc atcatgacgc gccccgaggc cgccccgcag
     1441 gccgtgtcga atttcgccag ccgcgaatgc gcggtgctaa tggccgattc ggccaacatc
     1501 ggcaccttca acgacgcgcg ctcgctgaag tatggcgtgt ccggcctgcc acactacccc
     1561 gaggccacca agacccccgg tcacccgttc gtgggcggtt cggccctgtg ggccatcaag
     1621 gggcacccgg cggcgcagga caaggccacc gcccagttcc tggcctggct cgccaagccc
     1681 gccaacgcgg ccaagtggta ccaggaaacg gggtatctgc ctttaacgac acaggcgttc
     1741 gacgccaccg gcgcgggcta ctacaagaac ctgggcgact ggcgcgccat cgtggccgac
     1801 tacgccacgc ggcccgacgc caccgagcgc ggtttctggg tcaacaacta cccgcagatc
     1861 cgcgtcatgc tgaagcagac gctggaccgc gcgctgaatg ggcaggaagc cgccgtgccg
     1921 gccctgcaaa ccgccaccag ccaggccgac cgcatggtgc gcgaacgctg atgcgatgtt
     1981 gtgggctgtc acgcccggca acccgaatat gggttgccgg gcgtttttgt cttacatgag
     2041 tcgaggcctg tgcttagtga ggttttgaga catcaaatga ttgaaaataa aagataaatt
     2101 tcactttgtg aaaccgtggg atcggttggg cgaaatgttc cgtccgtatt tttcgggctg
     2161 gcggccccgc gtcgatatgc ccaagatgag cgctcactga aacgggacgg caccacttgc
     2221 gtgttcgttc ggatatgaca ggtgggtgct gccatgcgcg ggtttccgat cacaattctt
     2281 tgtttactac tggttggctg tgggtcattg cctgactggg cgatattttc cccgcccgcg
     2341 gaaatgtcgg cacccgcccg ctatcacttc gattgggcgt tatccggcag ccgcgcggtg
     2401 gggccggagc aggtattcga cgacggtgag cggatgtggc tgcaattcac tgacggcgca
     2461 cccatacccg ccgtctttgc ccgctcggaa cggggtgata cgcctctgtc atatcgtcgg
     2521 gagggtcctt acgtcgttgt gcgcggcgtg tggccgcgac ttgtgctgcg tggaggtcat
     2581 caggaggcat ttgtgcgtcg cgttgcgccg cctccagatc agaccgtgtc ctcgacgccc
     2641 ccgaccatag cgaaaacggg cggcgtcagt gcgcgtcccg ctgtgggcaa ggccgtggcg
     2701 gatctgtcag ggccaaaggg tggtaagccg gccatgcgtg atggggcgaa ggccccgccg
     2761 cggatcgtga caagtaagga tgcggcgttt cccgaggcgt cccgccaggc gagcgacgca
     2821 cccgcgcagg tgcctggtac ggccgtggac tcccggcctg tgcccgcgat atcggtgata
     2881 cctcatagtt ctccgtcgcg cccgttgtcc gttgtggcag tcccatcccc gtcgcccggt
     2941 acggcgcaac cgtccggggc aaagatcgac gcgccgatca cgcgtgaata tttcgaggtt
     3001 ggtccggcgg acggcaacat tcgccagacc ctgaatcgtt gggcccaggt ggcgggatgg
     3061 acgttcaatg tggagcattg ggccgtgaat gtcgatgtcc cgatcgttgg ttcggccacc
     3121 ttcgactccg gattcaaacc cgcggtaagg gctttgcttg cctcgaccga gctgggggac
     3181 aggccattgc ggccctgctt ctattccaac aaggtggttc gcgtggtgcc ctacgcgcaa
     3241 gtgtgtgacc gcacacgcaa tccggggagc tcgtcatgag tcgtccacga cgcctggttc
     3301 tggtcgtggc ctcgtgcatg gtgttcacgg cgtgctcgtt ggcagaaaag gtgctggagg
     3361 tacgtaaatc cgctgatcgc gcccaggcaa agatcggagc tgagcaccgg gcgttcgatc
     3421 gggcagtggc cggggagggt gagcgccagg ccgcccagga ggtggatcgt ccgtggatag
     3481 ccgggcgccc ggttcctctt ggccgggacg tcacactgcc gtcggcgttg cgcgcgaatg
     3541 ttcgcaccac catgttgttc gcgggcgggc cggttgacct gcgccaggct gcggggcgca
     3601 tcacggccgc cacctccatt ccggttttcg tgcgcccgga tgccttgctg ccgttggagc
     3661 gttttttgcc gcggctatcg ggtgaggtat cgacgcctgc agcctccggc ggggtttccg
     3721 ccgaggtgat gatgggtttg ccggcgcgag aggcggagcc gcttgccgat attctcgatc
     3781 gtattggggc gaggctgggt gtgcaatggc ggtatacggc tggccgtatc gaatttttcc
     3841 gcactgaaac gcgggttttc gacgttcggg cgctggcact caatgccagc gcgcaggcat
     3901 ctctcggact cgaggggcgc ggcaagcagc aaggcttcgt cagcagctcg cgcacccgcc
     3961 tggagggtgg cgagcagaac cccatgaagg ctatccacgc caggcttgaa ccgtttctga
     4021 cgcgcgccgg tattctcgtg gcgcagcctg gcgccggcgc gtcggtggtg gtcacggata
     4081 ctcccgatgt tctgcggcgc atagcgacct atctcgacgc ggaaaatcgg gccctcacgc
     4141 gcagaatccg cctggttttc gaagagatca cgctcgcgac cgatgacagc gcagaggcgg
     4201 gaatagactg gaatgtggta ttcaccgatg cccgcctggc tgcctctttg tctatcgccg
     4261 cgccggttgc ggccgaggcg gcgagaatgg cgcttggcgc aacccacgga aactttcagg
     4321 ggtccgaggc cgtgatccgg gctttgagca aggtgggcag ggtggtgcgc cgaagcagtg
     4381 tacccgtgtt gacgctgaat cgacggccgg tgactcatgc ggtccgaacc acgttttctt
     4441 atattgacca ggttcagacc accgcgctgg ccaacggatc ggggttggcg ctaccgtccg
     4501 tgtccgtcag ccagcgggac gaaaccgtgg ggtcggtgct cacgctggtg ccggacgctc
     4561 aggacaacaa tcagatcctg ctatcggtgg cttatgacaa tacggtggcg cagccactca
     4621 aatccgtcac atttggcgat cgcagcaatc cattgcagtt gcaacaaatc acaatagatg
     4681 gcaatggcac cgtccagcaa gtattgcttc atcccgggca gccgctggtc atttccggat
     4741 tcgatcggaa gcaggagcag tccgaggggc gccgactcaa tccagggctt ccggccatcc
     4801 tcggcggcag tgatcaagcg tcgaagcagc ggttgatgac agtcatcatc atgtcggcgc
     4861 aagtcgaaga agatgcatga ccaaggaatc gacatggccc catacttgat cctgccctcg
     4921 tcggatagcg cactggtttt cggaatggag tggcttcccc tgatcggtcg ccgtctcgag
     4981 agcgccgcgt atcgcaccgc gcggcagtac aaggcgagcc atctggtttt cagcaacaca
     5041 ggggccacgg cggtggggtt ggcggacctc ccgactggaa tgccgcgggc cggcggggcg
     5101 ctccggtccg cggcccagtg tctggcgtgc ttgttttcct cgggtacggt ggcgctgatg
     5161 ctcgaggttg agtccggccg gttctgggtt gccgctgttc acgatggcgc ggttgtcacc
     5221 ggcacggacc gtttgtatcc gggcgaggat gtcgcgcgcg ccgcgatgga ggatctgaag
     5281 caggcttacc ctcgtttggc cgttcttggt gctcccggtt caccgcccgc cccgtccctg
     5341 cgggcattgg actccgccga tggaacgagg acgcgactgg tgcgcgcacg atcaggcgga
     5401 ggcattcggc gaaccttgat ctgttcgtca ctggcgatgc tgatggtggc gccgctgatt
     5461 atctgggggg tgcgcccgcg gaacgtggcg ctcgcgccaa cagcgttgcc agccgcgcac
     5521 cagagcccgg cgacgatctg gagacgtgct gtcgactccg cccgccaagg gcacgtcgtg
     5581 cacggctatg cggggacgcg gctggtcctg gacagctttt acgagttgcc cggaagcatg
     5641 gcgggatggt ctttgactca ggcggcttgt tcaccggaag ggtcgggctg gcgttgcgat
     5701 gccatgtatc gccgtagggc gaacggcgcc ggcagcgatg tgttcctgga tgcggcgccc
     5761 agtgcatggc gggtgtcgtt tgccggcctg gatcaggcac gtgtgtcgtg gcctctgagc
     5821 ggtaccgctg tcgcgctcgt gcgccacgac ccacactctg tgcgtcatga tgaccgtcat
     5881 ttactcagcc agcttcaggc actgtctccc gctttcacac ggctggaact cgggcagccc
     5941 gtggcattgc ggattacgcc gccacgggac cggggcggga gagtgccgcc tcggccgccg
     6001 ggcatgccac ggtacgtcac gcgatccgtg cgcatcatgg ggccgttgcg gtcagcgggc
     6061 atgctggctc cattgatgcc atcgatcgag tggtcacgcg tcaccttggc atcgagtgat
     6121 gtgcgcgcac catcgttgac cgccagtcgt ttcgtgatct ctttcgaagg agtcctgcat
     6181 gaatcagagc ctcttgatgc ggagggtgtc tcctccggcc gcgtgtccac cgttggccag
     6241 gaggataagt cgccgcagtg catgcatgac cgttgccacg gcgctggccc tgcaggcgct
     6301 tccggcgtgg tcggcgacgc cggctcaggc caacacagag ggggacggga tgttggtgcg
     6361 tgaattgatg cgcctggaga ctcggcttgc cctccgtcag gcacgacagc gtgctcaggc
     6421 cgccgaaacc catgggacac cgacgtcggc ggcgacttct tctgatcttg atctggaatt
     6481 gcaggcgatc tatggcgtgg gcaacaagct gttggcgcag gtgaggcata acgaacgaac
     6541 gctgatctat ctgcgaggca gtgccctgcc ggtcggtgtc aaagacgctg cgggcaatca
     6601 ggcattccgg cttgtgggtc tctcagggac ttgcgtccaa ctcgcccgcg aagcggtatc
     6661 ccatactctg tgcctgaccg gctcagggtc ggcggggaga taagcatgtg gccgatacgc
     6721 acagcctcga cgttgcgttc tccgtccgcc ttcgagccga tgagcgcgcg cttcgatgcc
     6781 gccacggccg tcgcgctcgc cggcgccgat gatctgttgt cgctggagcc ggcgtttctt
     6841 gcccccatgc atgacgagtt cggcgtggag gctctggcgg cgaaagtctg tcccgtgttg
     6901 ctggccgatc attcggtggc gatcttcgcc gtcacagggc acgaaggcag cgatcaggcc
     6961 gacgccttgg cacagcgtgc ccggcagcat ggttaccgtc tggccgaacc gcagcggtat
     7021 gtggtggccg cgccgctgct gctcgccatc gtgaggcgtc agttcgccgt gccgtcaacg
     7081 cgcccggttg gtggacatat gccggaggct tccagaaccg ctcttgccga ggcgtttcaa
     7141 gacatgattg agtggggcgt gcggcatggc gccagcgata tgcatctaaa tgtcgacctc
     7201 gatgagcccg agtccgaagt tcgatatacg gtgggcggaa ggtatgtggc gccggagtgt
     7261 tttcgccgca ttccaaccag cacactgatg gacatactcg ccgtggcctg gatggacatc
     7321 cgaggcggca acggcgccgt tttcgatccc acgatggaac agcaggggcg tttgacgcga
     7381 cgtgtcgacg gccgtgccgt cgtgttgcgc tgggcgtctc tggccgccga tcgcggcccc
     7441 agtgtctgcc tgcgcctact tgaacgcgag cccgcgatat cgtccctgtc tctggaggct
     7501 ctcggctatt ccgtcatgca gatcgccgtc atggagcggg ctgtgcaggc tgagggtggc
     7561 gcgattgtgt tttcagggac ggtagggtcg ggcaaatcga cgacactggc gtcgttgatc
     7621 acatctcttc ccgagtcgcg caaggttgtg actatcgagg atccggtcga gtacgtcatt
     7681 ccgcgtgcga tacaaaatac ggtcgcgcgg gatctcctgg ccgaagataa ctctgtttat
     7741 atggccaagc tgatggcgat caagcgttcg gcgatgacgg acgtgctatt gggcgagatt
     7801 cgtgatcagc agacggggcg cgccttcatg gacctcgcgg gctccggcgt gaacctgtac
     7861 accacggtgc acgcgccctc ggccgcactg accggtgaac ggttggcttc ggacttcatt
     7921 ggcgtttccc gggatttcct ggccacgcca ggggttctga agttgatcgt gcatcagtcc
     7981 ctgttgtcga gattgtgcgg ccactgctca ctgccggcaa cgtcaccggc cgagtgcccg
     8041 cagggttcta aaccgagcag ctggcggcaa tggctggcgc gcatcgagaa gctgtatgaa
     8101 cgatctgccg attcgctgcg cgtgcgtaat gagaagggct gcgcgcattg cctgaatccc
     8161 caggccccgg cgctcgcggg ctatgccggc aggactgtgg tggcagagtg catagagcct
     8221 cggcgtcatc ccgattttct tcggactctg cgtgatgggt gtccgggagg tgatctccgg
     8281 aacacgccct cgtcatcgga gtcctccgct ttatatgacg cgatgagcaa ggcgtttcaa
     8341 ggggatatcg acccacgtga catcgagtcc aggtttcctc ccatcgaggc tcagggtcat
     8401 catccccggg cgttcacgaa ggccgggccc ggctgatgcg cttcgctcca ttcctcttgg
     8461 tgcgtgatgt gggtgtccgc ctgcgcgacg agatggaccg gcggcggttc gatcgggcgc
     8521 gtgctgatta ctacgagtat ttggcggcgg tggtgggagc gacgcaggga cgacgcacag
     8581 tcaaggagat attcaatgcc gatgccctgc gctatggtcc ggatacagtg cgtgggcgcc
     8641 tttcccggcg ttggctcagc gcctatgagg ccagtggtgg ggatttgtat tcgacatggc
     8701 ggggttgttt cccgctttcg gaattgaatc ttgtacgcgc ggcccagcat ctcggcaatg
     8761 attccctggt acgaaccctt ggcgatctgg ccgctgctct gcgattgtcc cgccaggcgg
     8821 cgaacatact ggtatccacg ttgtggtcgg ccggcgtggc gttgatcgtg ctggccggca
     8881 tgtctttcgc ggtgccggcg ttcaccattc cgagtctcag gcagactttt cacgttttga
     8941 ccccagatta ttacggcgcc atgacgacgg cgttgtttgg attgtcggac tccattcgcg
     9001 acaagtgggt attggtggcc ggattgatgg cgggcgggtt ttgcgcgatt ctctggtcgc
     9061 ttccaaattt atgcgggccc gcgcgggtaa tggcggaaaa gctgacactg tggcgcattt
     9121 atcgccacat tcatgctctg tggttctttt cggctcttgg cattgtcctg ggaaaggatg
     9181 gcgcgtcgcc cacgcgattg cgcaccgcgc tatggatgca gaaggcgggt gcttcgccct
     9241 ggcgcaatgg gcacatcgac gccatgctgg ctcgcatcga tgccggcctc gtcggcgccg
     9301 ccacattcga tacagggttg ctggatcgag acctctactg gttcttgtgc gacaccgagc
     9361 ttgccagagg gctggctgtg gggctggtca tgacgcggca acgtctggaa cttcgggtgt
     9421 tgcgtgaagt ggccgcccgc gcggcgaggt tgcgctggtg cctgctgttg tcggccgtgg
     9481 ccgggctggt tggcctgggc gtgtggcatt acgcagtcat tgatgaattg cgtcgttctc
     9541 tgttgatctt ctatgccagc caatgaggcc ggcgagacaa ccatcgtttc gttcacgttt
     9601 ccctcactca aggagtcgac catgaccatt gccgctttcc gttttcgcca cacagcgcgt
     9661 cagcgcggat tttcattgat agaggtgtcg atcgtcaccg cgatcgtgct cttgcttgcc
     9721 atcatcggta ttccggccat tggcggctat gtggtggaga acaaagtgcc caaggtggga
     9781 gaagaactgg cgcgattcat tctccaggtc aaagtcaatg cgtcggttgg agcggggcaa
     9841 ccgtacgccg atatcgacac ggcgcatttc gcgaacctgg tgcgggattc aggtattttc
     9901 tcagtgacgg gggtcggggc gggtgcccag gtgctgcacg gcctgggaaa tgacggccgg
     9961 gtcacggtcg ctccgcaagg ggcgggtgaa tccttttccc tcgagttgac gaacgtgaac
    10021 cacgccgcgt gcccggcgtt ggcgtcggtc atgcagcggg tggtcgacac gatcagcgtg
    10081 gcgggagagg gccaggcggg ggcaaccgtg aaggacgccg ccacagccta tagcgcgctg
    10141 gccaccgagg cccaatgtgt gcagggggac cgtaatacgt ttgtgttcgt ggccagttga
    10201 gccgaggcgg agtatgacac gccagcgtgg attcgccctg ctcgagttgg ttgtcgccgc
    10261 gttgatcacg acgttgttgg cggtatgggg cgccaatgcg tgggttcatc gactcgatga
    10321 ggccaatgcg cacgccatgg ccgcgtggat gctcgcggcc cgggagggtg tgcaagcgta
    10381 cattcaccgt catggaatat cgatggcgca aatggacgcg ggtggcacag aatccgtgcc
    10441 gggtttccaa gactggcgca cccccacggt cccggaactc aaaggggcag gcctgttggc
    10501 cgacgggttt cctgaagccg ggcctggcgg attgcgtgtg gcggtgcgga ttctttccgg
    10561 aaagaattgt ccaggttccg attgctcgct ggaggcgctg atccttggat caccgccgct
    10621 gctggacaag cgatctgggg aggtggacga acagaaggtt gccctgtggc tgctggcgtc
    10681 acgagggttg ggaggtgtgg tgaatccccg ggaccccgct tccgtacggg gcgccgcgtt
    10741 tggctttgcc aatccaccag ccgccggcat gtcgatgttg cctgtcggca cgcccgccat
    10801 ggccatcacg accgcgggct catccgacgg cgcacagttt ctgcgcgtgc gggatgacag
    10861 ggatcccgac tttcagggaa cagcgtcggt gcgcggtggc atccgctctg acggttccgt
    10921 gaccgccgga gaatatgtga tcgtcggcgc gcgtcacact gtcaggtcgt cgtgcgggca
    10981 agaaggcgcg gtggcccgcg atgcatatcg cggtttgctg atttgccaga gcggtctgtg
    11041 gcgttccgcc ggtggaagtg gaggcggcgg attctccacc aacaccgtgt atggctgctt
    11101 tacgtcaagc ggtctgtcca cggccaatcc ggtgacggga tcgtgcaact gcccgctcgg
    11161 ccatgccatg gtggcgatct ccgacagcgg ctcggcggct tcgccagacg gccgaacgcg
    11221 cggctacctg tgcgtggaat aggggttgcc cggccaactc cctctataat caaacgttac
    11281 ctgttcaaat cgcgccattc cgcaatgaag acctccgaga tccgtcagaa attcctctcc
    11341 tttttcgagt ccagggggca cc
//
LOCUS       DASTNK010000114        13508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1013406, whole genome shotgun sequence.
ACCESSION   DASTNK010000114 DASTNK010000000
VERSION     DASTNK010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13508
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013406"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VFR20_08390"
     CDS             <1..327
                     /locus_tag="VFR20_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU0230577.1"
                     /translation="QGVSNDALMVPQQAVQRTADGLSTLMLVKDGKVSPQAVELGSQV
                     NGKWIVTKGLNPGDEVIVEGFQKIRPGVPVQPLPWKNPTEASAAGAKPAAAPAEKGAG
                     TPPKQG"
     gene            393..3617
                     /locus_tag="VFR20_08395"
     CDS             393..3617
                     /locus_tag="VFR20_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0230578.1"
                     /translation="MPQFFIDRPIFAWVVALGIALAGLLSIPNMPVSQYPDVAPPAIQ
                     IQASYPGASAEDVSTSVASVIENQLNGAKGLLYYESVSDSNGQAQITATFKPGTNPDL
                     AQVDVQNRISNVTSQLPEAVIQQGLKVSQVNTGFLMVVTLSSKDGSLDQTALADYVTR
                     NIQNSVSRVPGVGQFQLFAAPRAMRIWVDPDKLVSYNLSMAQVMTAIRQQNVLISGGI
                     LGSPPNPASQRVTAPIVVNGQLSSVKDFGNIILRSNVDGSAVHLSDVARIEVGADNYQ
                     FGARLNGKPTAAFAISLSPGANALSTAEGVKHEMEQLSKFFPDNITWAIPYNTAPYVQ
                     ISIEQVVHTLLEAMVLVFVVMFLFLQNVRYTLIPALVVPVAMMGAFTVMYALGFSVNV
                     LTMFAMVLAIGILVDDAIVVVENVERIMAEEGLPPAEATKKAMPQITGAIIGITLVLS
                     TVFLPLAFMSGSVGIIYRQFSVAMAVSILFSAFLALSFTPALCATLLKPIPKGHHESK
                     TGFFGWFNRGFGATTRHYESWVGRSLHRGGRMMLVYLVLIIGLGWMYLRLPSSFLPEE
                     DQGYLIANIELPAGATANRTLEVIKKVEDYFKKIPAVKNMITVQGFSFNGNGLNSAIA
                     FVTLKDFSERKKPGESAMAIAGAAMPKLLFGLPDSMVLSIVPPAISSLGNASGFDMRL
                     EDRGGVGHAALEAAAGQLLQMASKDPVLSQTRITGLGPGPQLKLTIDRKKAAALGVDF
                     SEAAQLISSAIGSGYVSKFTNMGWVQNVWVQADEAHRMNLGELMKLEALNGKGQMVPL
                     SAFVTAKWERAPVQIVRYNSYDSIRLGGSAAPGYSTGQAMDEMQKLVAKLPGSFGYDW
                     TGLSYQEKQAGSQSGILLGLAMLVVFMVLAALYESWAIPLSVMMAVPLGMLGAVAMIA
                     AKGMSSDVYFQVGMVTVIGLAAKNAILIVEFAKDKYAMGEDIYESTIEAARLRFRPIL
                     MTSFAFILGVVPLVMANGAGAASQNAVGLGVMGGMLAATPLAVLFVPTFFVVVLRFFK
                     TKPKLLGAHADGAVKSSGDGHHHPTASETSPPGASALPTEGDQS"
     gene            3614..5143
                     /locus_tag="VFR20_08400"
     CDS             3614..5143
                     /locus_tag="VFR20_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEU0230579.1"
                     /translation="MSRGALRTAFPLAAVALALSGCSFAPKYQRPVAPVPDQYPAYSG
                     LPSGHSTAGVKASNVQLAADLGWQEFFRDPELKKLIGIALANNRDMRVAVDRVEVARA
                     QYGITQSDRWPTIGLGAQGSIVGSPQSIRAPNTNSVSRVYQAGIGMTAFELDFFGRLK
                     NLSEAAYEQYLATDQARRSVHINLVSQVAEAYFRLRAAQEQYKLVRKTLGSYQQSYNL
                     VKSRYDAGVASALDLNQAESQLNSAQADLQSTMRGQEQARNALLLLLGTRAIPDLSHA
                     APFDRDQLVSAVPVGLPSQLLERRPDIAGAEDQLRAANANIGAARAAFFPNISITGLL
                     GFASSQLGGLFGSQNHYWSLSPSVQIPFLSGSVINNLALAKANKKIAVAQYEKSVQTA
                     FREVADALAGEATYSRQLDALRALQKTSFATLDLARLRYNTGVDSFLQVQTAEVNLYT
                     VQNNLLQVGLNSLLNRVDLYAALGGGWLKDTTAAAANTAGGRAIAADAAKQNTAGSKH
                     D"
     gene            5136..5858
                     /locus_tag="VFR20_08405"
     CDS             5136..5858
                     /locus_tag="VFR20_08405"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEU0230580.1"
                     /translation="MIELGVNIDHVATLRQQRLTVYPDPVRAALCAEDAGADLITLHL
                     REDRRHIQDADVIAVRAALRTRMNLECAITPEMLDIACRVKPDDVCLVPEKRQELTTE
                     GGLDVVGRYAAVERAVARLREAGVRVSLFIDPDPAQIAAAARAGATVIELHTGAFADA
                     GNAEAARHELERVRAGIAQGIGLGLRVNAGHGLHYDNVLAVAALPGVSELNIGHAIIA
                     RAVFDGLDRAVRDMKALLVSVG"
     gene            5929..6510
                     /locus_tag="VFR20_08410"
     CDS             5929..6510
                     /locus_tag="VFR20_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEU0230581.1"
                     /translation="MSDNPIQSLLSRQSVKFVRAPGPTEEHLSLILQAAMRAPDHGKL
                     RPWRFALVRENAVGRFADLAIALINASDWPMPPEKEASTRRWLSQVPLLIAIACRMDT
                     TNQRIPEHERMLATGAAVTNMLSAAHMLGYGAYWSTGLGTYNDEVAAALGFDALDYRF
                     MGYLAVGTPIDPPPRTERPDWSEFVTDWSGPVA"
     gene            complement(6580..7143)
                     /locus_tag="VFR20_08415"
     CDS             complement(6580..7143)
                     /locus_tag="VFR20_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEU0230582.1"
                     /translation="MAHAAPAAKGPASTMHDIRGFLPDLRFTLEGAGGKVVTQNAFKG
                     KVVLLYFGYSNCPDVCPTTMAQLAQVMKNLGSEAVDARIVFVSVDPHRDTPDKLQAYV
                     DIFYKDAIGLTGTEEQIADIARRYRVAYQIEKPTTTDKNDYAVSHSRGVYIFDQRGHA
                     RLLASGTESIAILTQGIKRIIEHPAKN"
     gene            complement(7436..9517)
                     /locus_tag="VFR20_08420"
     CDS             complement(7436..9517)
                     /locus_tag="VFR20_08420"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEU0230583.1"
                     /translation="MTPNPLLAPVDALIDYDAILPEHIEPAIDHLLGVARAAVETAAD
                     TGTTASWPAFVDPLEEAVEPLWRAWSVVSHLNAVVNTPALRDAYNQCLPRITEFSTWT
                     GQHVGLHAQYQRLRAGDDYPRLTPVRQRIVDLALRDFHLGGVDLHGAERERYAQLSEQ
                     IATASQKFSENVLDARDRWALYIEDEARLEGLPPDVQRTARQAALAEGREGWKLTLKM
                     PCYLPVMQYARDRALREACYRGHATLASEQGDPAFDNTTLIEQILALRSEEASLLGYT
                     SYAELALQTRMANQPTEVVDFLLDLAHRAAPYAHRDLDELRAFAKDHLGMDEPAPWDI
                     AFASERLRESRYAYSDDEIKRYFTEPRVLQGMFEVVARLFGVTARPVPARVWHPDVQA
                     LRIETAEGELRGHLFLDLYAREGKQGGAWVDAERNRRKTATALRTPVAYLTCNFSRPD
                     GDNPALLTHDDVITLFHETGHALHCLLSQVDEPAASPFSAVEWDAIELPSQFMENFCW
                     EWPVLSKLSAHVDSGVALPRPLFDKLLSARNFQSGMQTVRQIEFGLFDMRIHQHSTGL
                     TMARVSETLDAVRQNVAVLFPPPWHRFAHGFSHIFAGGYGAGYYSYKWAEVLSADAYA
                     AFEETATPDSGGGRDTLNPQTGRRFLQEILAVGGSRPATESFIAFRGRPATTEALLRH
                     SGMAPGGSARH"
     gene            complement(9546..10172)
                     /locus_tag="VFR20_08425"
     CDS             complement(9546..10172)
                     /locus_tag="VFR20_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0230584.1"
                     /translation="MTTPQVPSTIFIVDDDEAVRDSLRWLLEANGYRVRSFDRAEEFL
                     SAYDPEQIGVLIADVRMPGMSGLELQEELIARKAPLPIVFITGHGDVPMAVSTMKKGA
                     VDFLEKPFNETDLRTIVARMLRQASDRVSQAQAEKNHQEVLARLTTREQQVLERIVAG
                     RLNKQIAGDLNISIKTVEAHRANIMEKLEVTTVADLMKIALTKEKSVA"
     gene            complement(10169..11926)
                     /locus_tag="VFR20_08430"
     CDS             complement(10169..11926)
                     /locus_tag="VFR20_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU0230585.1"
                     /translation="MASPSKKSLIPTTFYDQANNSRRGFYWLTPVIVLVLYLCVMGVF
                     IWLQRLHNDSVMFVTIDQETRQQRLLLVVVALSCVIVLSLLALWRYTRFRSRAESALI
                     AETGFRRAMENSMSTGMRVFDMHGRIAYVNPAFCRMIGWNEADLIGRTTPFPYWVPGR
                     YAQHQETLELLLSGRTPSSGLEIEAQRRDGSRFTARMYVSPLRDPNGEQIGWMTSMTD
                     ITEPKRIREALTAAHERFMTVLEGLDDAISVVADTADGLELLFANRTYRRFFGAQSGG
                     HAELLGGRLGRFTDETIEAFSESSQRWFEVHHRILAWTDGRRVRLQVARDITERRRHE
                     EASRVQQEKIQLTSRLTTMGEMASSLAHELNQPLTAISNYSMGAVAMLKSGNPQPDKL
                     LVALEKAAAQAERAGKIISRIREFVKRSEPRRQRVVVTRIVDNAIGFADIDARKRQIE
                     IQPHFPDPLPEVMADPILIEQVLLNLLKNGVEAMEKSEYRILHVVVSDQSPLVEIAVI
                     DRGYGLRDPERLFEPFYSTKSEGLGMGLNICRTIIESHHGRLWAAANPDGGTIFRFTL
                     PCAAPQVPDNADTPQELQV"
     gene            12202..>13508
                     /gene="aceE"
                     /locus_tag="VFR20_08435"
     CDS             12202..>13508
                     /gene="aceE"
                     /locus_tag="VFR20_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799716.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEU0230586.1"
                     /translation="MSSLEPTTAVAAADEALETQEWLEALESVLDREGPERAHFFIEK
                     LIDLARRSGAHIPYSPNTAYLNTIPPGLEPAYPGNLVLEERIRSYIRWNAMAMVVKAN
                     RLHPADGGDLGGHIASFASLATMVACGQNHFWHAETPDHGGDLVYFQGHSSPGMYGRA
                     YLEGRLTEGQLDNFRQEVGGKGLSSYPHPKLMPDFWQFSTVSMGLGPLMAIYQARFLK
                     YLHARGIADTSKRKVWVFCGDGEMDEPESLGAIGLAAREKLDNLIFVINCNLQRLDGP
                     VRGNGKIIQELEGDFRGSGWNVIKLIWGGYWDPLLARDKEGILRRVMEESVDGEYQAY
                     KARDGKFVRDHFFGKHPKLLEMVSRMSDEDVWRLNRGGHDPYKVYSAFAAASAHKDQP
                     TVILAKTIKGYGMGQVGQAKNPTHQQKKLDTESVREFRDRFNIP"
BASE COUNT         2506 a         4010 c         4287 g         2705 t
ORIGIN      
        1 cagggggtca gcaatgacgc gctcatggtg ccgcagcagg ctgtgcagcg cacggccgat
       61 ggtctcagca cgctgatgct cgtgaaagat ggaaaggtct ccccgcaggc ggtcgaactg
      121 ggttcgcagg tgaacggaaa atggattgtc accaaggggc tcaacccggg cgacgaggtc
      181 attgtcgagg gattccagaa gatccgtccc ggagtgcccg tgcaaccctt gccatggaag
      241 aatcccaccg aagcgtccgc cgccggcgcc aagcccgccg ccgcgccagc cgagaagggt
      301 gctggaacgc cccccaagca aggctgacaa ggctgtcccg gctcccgtcg actcgggagc
      361 cgacgacttc aatgctttaa aagtgagcga acatgccgca gtttttcatt gataggccca
      421 ttttcgcctg ggtggttgcc ctgggcatcg cgctcgcggg tctgctgtcg ataccgaaca
      481 tgccggtgtc gcaatacccc gacgttgcgc caccagccat tcagatccag gcttcgtatc
      541 cgggcgcttc cgccgaagat gtgtccacgt cggtcgccag cgtcatcgag aaccagctca
      601 atggcgcgaa aggcctgttg tattacgaat cagtcagcga ctcgaatggt caggctcaga
      661 tcacggccac gttcaagcct ggtacgaacc ccgacctggc acaggtggat gtgcagaacc
      721 ggatatccaa cgtcacatcc cagttacccg aggccgtcat tcaacaaggc ctgaaggtca
      781 gccaggtcaa taccgggttc ctgatggtgg tcaccctgtc gtccaaagat ggctcgctcg
      841 accaaaccgc gctggccgac tatgtgacac gcaacatcca gaactcggtg tcgcgcgttc
      901 ccggtgtcgg gcagttccag ctgttcgccg cgccgcgcgc catgcgtatc tgggtcgatc
      961 ccgacaaact ggtgtcctac aacctcagca tggcccaggt gatgacggcc atccgccagc
     1021 agaacgtctt gatatccggc ggtattctgg gttcaccgcc caatccggcc agccagcgtg
     1081 tgaccgcgcc cattgtggtc aatggacagt tgtcctcggt gaaggacttc ggcaacatca
     1141 tcctgcgctc gaacgtggat gggtccgccg tgcacctgtc cgacgtcgcc cgcatcgagg
     1201 tcggcgccga caattaccag tttggtgccc gcctcaatgg caagcccacg gcggcgtttg
     1261 ccatttccct gtcgcccggc gcgaacgcgt tgtcgaccgc ggaaggtgtc aaacacgaga
     1321 tggagcagtt gtccaagttc ttccccgaca acatcacttg ggccatcccg tacaacacgg
     1381 cgccgtatgt gcagatttcc atcgaacagg tggtgcatac gctgctcgaa gccatggtgc
     1441 tggttttcgt ggtgatgttc ctgtttctgc agaacgtgcg ctatacgctg attccagcgc
     1501 tcgtcgtgcc cgtggccatg atgggggctt tcaccgttat gtacgcgctg gggttctcgg
     1561 tcaacgtgct caccatgttc gccatggtgc tggccatcgg gattctggtc gatgatgcca
     1621 tcgtggtagt cgagaacgtt gaacgcatca tggccgagga agggctgccg cccgccgagg
     1681 ccacgaaaaa ggccatgccg cagatcacag gcgccatcat cggcatcacg ctggtgctgt
     1741 ccacggtgtt cctgccgttg gcgttcatgt cagggtcggt ggggatcatc taccggcagt
     1801 tctctgtggc catggcggtg tccatcctgt tctcggcgtt cctggcgcta tcgttcacgc
     1861 ccgcactgtg cgcgaccctt ctcaaaccca tcccgaaggg gcatcacgaa agcaaaacag
     1921 gcttctttgg gtggttcaat cgcgggttcg gcgcgacgac gcgccattac gaaagctggg
     1981 ttggccgcag cctgcatcgg ggcgggcgca tgatgctcgt ctacctggtg ctcataatcg
     2041 gtctgggctg gatgtatctg cgccttccgt cgtccttcct gcccgaggaa gaccagggct
     2101 acctgatcgc caacatcgaa ctgccggccg gcgcgacagc caatcgcacg ctcgaggtca
     2161 tcaagaaagt cgaggattat ttcaagaaaa tccccgctgt gaagaacatg atcaccgtgc
     2221 aagggttcag cttcaatggt aacggtctga actccgccat tgcgttcgtg accctgaagg
     2281 atttttccga gcgcaagaag cctggcgaat cggcgatggc cattgctggc gcggccatgc
     2341 ccaaattgct gtttggtttg ccggattcca tggtcctttc catcgtgcct cccgctattt
     2401 cgtcgttggg aaacgcgtcg ggcttcgata tgcggctgga agaccggggc ggggtagggc
     2461 atgcggcgct ggaagccgcg gcggggcagt tattgcagat ggcctccaag gatccagtgc
     2521 tgtcgcagac acgcatcacc gggcttgggc ccggcccgca actcaagctc accattgacc
     2581 gcaagaaggc cgcggcgctt ggggtggatt tctccgaggc ggcgcaactc atctcgtccg
     2641 cgatcggctc gggttatgtg agcaaattca ccaacatggg ctgggtgcag aacgtctggg
     2701 tgcaagccga cgaagcccat cgcatgaatc tcggcgagct catgaaactc gaggcgctca
     2761 acggcaaggg ccagatggtg ccgttgtcgg cgtttgtcac cgccaagtgg gaaagggcgc
     2821 cggtgcagat cgtccgttac aacagctacg attccatccg cctgggcggg tccgccgcac
     2881 ccgggtattc cacagggcaa gccatggacg agatgcaaaa actcgtggcc aaactgcccg
     2941 gcagcttcgg ttacgactgg accgggctgt cctatcagga aaagcaggcc ggcagtcaga
     3001 gtggcatact gctcggtctg gccatgctgg tggtgttcat ggttctggcc gcgttgtatg
     3061 aaagctgggc gattccgttg tcggtcatga tggcggtgcc gttgggtatg ctgggggccg
     3121 tggcgatgat cgcggccaag ggcatgtcca gcgatgtgta cttccaggtc ggcatggtca
     3181 ccgtcatcgg cctggccgcg aagaatgcga ttctgatcgt ggagttcgcc aaggacaagt
     3241 acgccatggg cgaggatatc tacgaatcga cgatcgaggc ggcgcggctg cgctttcgtc
     3301 ccatcctgat gacgtcgttc gcgttcattc tgggcgtggt gccgctggtg atggccaatg
     3361 gcgcgggcgc ggccagtcag aacgcggtgg ggctgggcgt catggggggc atgcttgccg
     3421 ccacgccact ggctgtctta ttcgttccaa cttttttcgt ggtggttctg agattcttca
     3481 aaaccaagcc caaactactt ggtgctcatg ctgacggggc ggtcaaatcg tcaggagacg
     3541 gacatcatca tccaaccgcg tcggagacgt cgccgccagg tgccagtgca ttgccgacag
     3601 aaggagatca atcatgagtc ggggcgcgct tagaacggcg tttccgctag ccgccgtggc
     3661 gctggcgctg tcggggtgct cgttcgcgcc caaatatcag cgcccggtgg cgcccgttcc
     3721 ggatcagtat ccggcgtatt ccgggctacc gtcgggtcac agcacggccg gggtcaaggc
     3781 ttcgaacgta cagcttgccg ccgatctcgg gtggcaggag ttcttccgcg acccggaact
     3841 caagaagctc atcggtatcg cgctggccaa caaccgcgac atgcgggtcg ctgtcgatcg
     3901 ggtggaggtg gcgcgcgcgc agtacggcat cacccaaagc gaccgttggc ccaccattgg
     3961 cctgggggcg cagggttcca ttgtgggcag tccgcagtcc atccgcgccc ccaataccaa
     4021 ttcggtatcg cgcgtttatc aggccggcat aggcatgacg gcgttcgagc tggatttttt
     4081 cggtcgcttg aagaacctca gcgaagccgc ttacgagcag tatctggcaa ccgaccaggc
     4141 acgtcgaagc gtgcacatca atctggtttc gcaggtggct gaggcctact tccgtctgcg
     4201 cgcggcccag gaacaataca aactggtgcg caagaccctg gggtcctatc aacagtccta
     4261 caacctggtc aagtcgcgct acgacgccgg cgtggccagc gctctggatc tgaatcaggc
     4321 cgagagccag ctcaattcgg cccaggccga tctgcaaagc accatgcgcg gccaggaaca
     4381 ggctcgcaac gcgttgctgt tgttgctggg cacgcgggca attcccgatc tttcccatgc
     4441 ggcgccgttc gatcgtgacc aattggtcag cgcggtgccg gtgggcttgc cgtcgcagct
     4501 actcgagcgg cgccccgaca ttgccggggc cgaagaccag ctgcgggcgg ccaacgccaa
     4561 catcggggcg gcgcgcgcgg cgttcttccc gaatatttcc atcaccggtc tgctaggttt
     4621 cgccagtagc cagctgggcg gcctgttcgg ttctcaaaac cactattggt cgctgtcgcc
     4681 gtccgtccag attccatttc tgtcggggtc tgtcatcaac aatctcgcgc tggccaaggc
     4741 caacaaaaag attgccgtgg cgcaatacga aaaatcggtt cagacggcat tccgcgaagt
     4801 ggcggatgcg ctggcgggcg aggccacgta ctcgcgtcag cttgatgcgt tgcgtgcctt
     4861 gcagaaaaca tcgttcgcca ctctggacct tgccaggcta cgctacaaca caggcgtgga
     4921 cagtttcctg caggtgcaga ccgccgaggt caatctgtat acggtgcaaa acaatctgct
     4981 ccaggtcggt ctgaattcct tgctcaaccg tgtggatcta tacgcggccc tgggtggggg
     5041 atggctgaag gacacgaccg ccgccgcggc caacacagcc ggcggacggg cgatcgcggc
     5101 tgacgcggcc aagcagaaca cggccgggag caagcatgat tgaactgggt gtgaacatcg
     5161 atcacgtcgc gacgctgcgc cagcagcgtc tcacggtcta tccagatccc gtgcgcgcgg
     5221 cattgtgcgc cgaggatgcc ggtgcggatt tgatcacctt gcatttgcgg gaggatcgcc
     5281 gccacatcca ggacgcggac gtgatcgcgg tccgggcggc gttgcgtacc cgcatgaatc
     5341 tggagtgcgc gatcacgccg gaaatgcttg acatcgcatg ccgtgtgaag ccggacgacg
     5401 tctgcctggt gcccgagaaa cgccaggaac tgacaaccga gggcgggttg gatgtcgtgg
     5461 gccggtacgc cgccgtcgag cgggccgtgg ccaggctgcg tgaggcgggt gtccgcgtgt
     5521 cgctgttcat cgatcccgac cctgcgcaga tcgcggcggc ggcccgcgcg ggcgccacgg
     5581 tcatcgaact gcacaccggc gcgttcgcgg acgctggcaa tgccgaggcc gcccgacatg
     5641 aactggaacg agtacgcgcc gggatcgcgc aaggtatcgg tctgggcctg cgtgtgaacg
     5701 cggggcatgg cctgcattac gacaatgtat tggcggtggc cgcgctgccc ggggtgtcgg
     5761 agctgaacat cggtcacgcg atcatcgccc gcgccgtctt cgacggcctg gacagggccg
     5821 tgcgtgacat gaaagctttg ctggtgtcgg tcggctgagc attcgtcgcc tggctcccgc
     5881 gtcccgggcg acgaacttcc cgaagccttt ttgattggaa accgttgtat gtcagataat
     5941 cccatccagt cgctgctttc gcgccagtcg gtgaagttcg tccgggctcc cggccccacc
     6001 gaagaacatc tgtcgttgat tttgcaggca gctatgcgcg cgccggatca cggcaagctg
     6061 cggccgtggc gtttcgcctt ggtccgcgag aatgccgtgg gccgtttcgc cgatctggcc
     6121 atcgccctca tcaatgcttc cgattggccg atgccgcccg agaaagaggc gtcgacccgg
     6181 cgttggctct cacaggttcc tctgctgatc gcgatcgctt gtcgaatgga taccaccaac
     6241 cagcgcattc ccgagcacga gcgcatgctg gcgaccggcg ccgccgtcac caacatgctg
     6301 agcgccgcgc atatgctggg ctatggcgcc tactggagca ctggcctggg tacctacaat
     6361 gacgaggtcg cggcggccct gggtttcgac gcgctggatt accgcttcat ggggtatttg
     6421 gctgtcggca cgcccattga tccgccgccc cggacagagc gtcccgattg gtcggagttc
     6481 gtgacggatt ggtcagggcc tgtggcgtga agggtggcga tcgctcggtc tcaacgaccg
     6541 tgcgtgatga atggcttcat gtgacagcct gaccgcatgt caattcttgg cgggatgctc
     6601 gatgatccgc ttgatgcctt gggtcaagat ggcgatcgat tcggtgccgg aggccagcag
     6661 acgggcatgg ccgcgctgat cgaagatgta gactccccgg ctgtgtgaca ccgcataatc
     6721 gtttttgtcg gtggtggtcg gtttttcgat ctggtaggcg acccggtagc gtcgggcgat
     6781 gtcggcgatt tgttcctcgg tgcctgtcag cccgatcgcg tctttgtaga aaatgtccac
     6841 gtaggcctgc agtttgtcag gggtatcgcg gtgcgggtcg acactgacga acacgatgcg
     6901 ggcgtcgacc gcctcggatc ccagattctt catgacttga gccaattgcg ccatggtcgt
     6961 tgggcagaca tccgggcaat tggagtagcc aaagtacaac agcaccactt ttcccttgaa
     7021 tgcgttctgg gtgacgactt tgccgccagc gccctccagc gtgaatcgca agtcgggcag
     7081 aaagccgcgt atgtcatgca tggttgacgc ggggcccttc gcagcgggcg cggcatgggc
     7141 catggcggca agcccgagtg acacggcgag aagtagcgaa gaaagcatgc tgcgcatgat
     7201 gaacgtccgg aaaatgcttg gtgaaaattg tcgatgtgct gtttagctgg ctcgttcgtg
     7261 tgttaagaac acactagtat cctgtttgat taattgggct gcttatcttc ggcgcctgga
     7321 ctggccatgc agcgttgcaa atcctcgcga tagccactgc tatcgctgcg gtttgcgcct
     7381 tgcctggcaa gcccatgcat ccgaatataa gtgtacgaac caatcaaaca gggcactagt
     7441 gtcgcgccga gccgcccggt gccatgccgc tgtgacgcag cagggcttca gtggtggcgg
     7501 gcctgccacg aaatgcgatg aatgattccg tcgcagggcg ggatccgcca acggccagta
     7561 tctcctggag aaagcgccgg ccggtttgcg ggttcaatgt gtcgcgtccg ccaccggagt
     7621 cgggagtggc ggtttcctcg aacgccgcgt aggcgtcggc ggacagcacc tcggcccatt
     7681 tataactgta atacccggca ccgtagccgc ccgcgaagat atgcgagaaa ccatgtgcga
     7741 accggtgcca cggcggcgga aacagcaccg ccacattctg gcgtacggca tccagcgttt
     7801 ccgaaacccg tgccatggtc aagcccgttg agtgctggtg gatacgcatg tcgaacagac
     7861 cgaactcgat ctgtcggacg gtttgcatgc cgctttggaa attgcgcgcc gacagaagtt
     7921 tatcgaagag tggtcgaggc agggcgacgc cgctgtccac atgagcggac agcttgctca
     7981 gaaccggcca ttcccagcaa aaattctcca tgaactgcga gggaagctcg atggcatccc
     8041 attcgacggc cgaaaacggc gacgcggcgg gctcatcgac ttgtgaaagc aggcaatgca
     8101 acgcatgccc cgtttcatgg aacagcgtga tgacgtcgtc gtgagtcagc agcgcggggt
     8161 tgtcgccatc aggccgggaa aaattgcagg tcagatacgc cacgggggtg cgcagcgccg
     8221 tggctgtctt gcgtcgattg cgctccgcgt cgacccacgc gccgccttgt tttccttccc
     8281 gtgcatagag atcgagaaac aggtggccac gcagctcacc ttcggcggtt tcgatgcgca
     8341 gggcttggac gtccggatgc cagacccggg ccgggacagg ccgggccgtc acgccgaaca
     8401 gtcgggcgac cacttcaaac attccctgca acacgcgcgg ttcggtgaaa tagcgcttga
     8461 tttcgtcgtc ggagtaggcg taccggcttt cccgcaagcg ctcggacgcg aaggcgatgt
     8521 cccagggggc tggttcatcc atgcccagat ggtctttggc gaaagcccgc agttcgtcga
     8581 gatcgcggtg ggcgtacggg gcggcacggt gcgccaggtc aagcaggaaa tccacaacct
     8641 cggtgggctg gttcgccatg cgtgtctgca gggcgagttc ggcataactt gtatagccga
     8701 gtagggacgc ctcttcgctg cgcagggcca gaatttgttc gatcagagtt gtgttgtcga
     8761 atgccgggtc gccttgttcg gaggccagtg tcgcatgccc cctgtagcag gcttcgcgca
     8821 gggcccggtc tcgggcgtat tgcatgacgg ggagatagca cggcattttc agcgtgagtt
     8881 tccagccctc gcgtccttca gccagagccg cttgtcgcgc ggtgcgttgc acgtctggtg
     8941 gcaggccctc aaggcgtgcc tcgtcttcta tatagagcgc ccagcgatcc ctggcgtcca
     9001 gaacattctc ggaaaacttc tgcgacgcgg tcgcgatctg ttccgacagt tgcgcataac
     9061 gttcccgttc ggctccgtgg aggtctacgc cgcccaggtg aaaatcgcgc agggcgagat
     9121 cgacgatgcg ctggcggacc ggtgtcagtc gtggatagtc gtcgccggcg cgcaggcgct
     9181 gatactgggc gtgcaggcca acgtgctgtc cggtccaggt tgagaattcg gtgatgcgag
     9241 gcaggcattg gttgtaggcg tcgcgcaacg ccggggtgtt caccaccgcg ttcaggtggc
     9301 tgacgactga ccaggctcgc cacagaggct ccaccgcctc ttcaagaggg tcgacgaagg
     9361 cgggccagga agccgtggtt ccggtgtcgg cggccgtttc gactgccgca cgggcgacgc
     9421 caagcaggtg atcgatggcg ggttcgatgt gttcaggaag gatggcgtcg taatcgatca
     9481 gcgcgtcgac gggggccagc aggggattgg gtgtcatgac ttggtatgcg gacgcggctg
     9541 gactgtcagg cgacactctt ttctttcgtg agggcgattt tcatcaagtc ggccaccgtc
     9601 gtgacttcga gcttttccat gatgttggcc cggtgggcct cgactgtctt gatgctgatg
     9661 ttcaagtcac cggcgatctg cttgttcagt cgaccagcaa cgatacgctc cagaacctgt
     9721 tgttcgcggg tggtgagccg ggccaggact tcctggtggt ttttctccgc ctgggcctgg
     9781 gatacccgat cggacgcctg tcgcagcatg cgggcgacaa tggtgcgcaa atcggtttcg
     9841 ttgaacggtt tttccaggaa atcgacggcg ccttttttca tcgtggacac ggccatgggc
     9901 acgtcgccgt gtccggtgat gaacacgatg ggcaagggtg ccttcctggc gatcagctct
     9961 tcctggagtt cgaggccgct catgcctggc atgcgcacat cggctatgag gacaccgatc
    10021 tgctccgggt catacgcact gaggaattcc tcggcccggt caaagctcct gactcggtag
    10081 ccattggcct cgagcagcca acgcaaagag tcgcggacag cttcgtcatc gtccacgata
    10141 aaaatggtgc tgggcacttg gggtgtggtc atacctgcaa ctcctgaggt gtatcggcat
    10201 tgtcgggcac ctggggtgcc gcacaaggca gtgtaaagcg gaaaatggtg ccgccatcgg
    10261 gattcgcggc cgcccacagc cggccatggt gcgattcgat gatggtgcgg cagatgttca
    10321 ggcccatccc caggccttcg gacttggtgc tgtaaaacgg ttcgaacagg cgttccgggt
    10381 cgcgcaggcc atagccgcga tcgatcacgg cgatctccac gagcgggctc tggtcggaca
    10441 ccacgacatg caatatacga tattcacttt tctccatggc ttcgacacca ttcttcaaca
    10501 ggttcagcag aacctgttcg atgagaatgg ggtcggccat gacctcgggc agcgggtcgg
    10561 gaaagtgcgg ttggatttcg atctggcgct tgcgcgcatc gatgtcggcg aagccgatgg
    10621 cgttgtcgac gatgcgcgtc acgacgacac gttggcggcg cggctcgctg cgcttgacga
    10681 actcccggat gcgactgatg atcttgccgg cgcgctcggc ttgtgcggcc gctttctcga
    10741 gcgccaccag caatttatcg ggttgcggat tgccggactt gagcatggcg accgcgccca
    10801 tgctgtagtt gctgatggcg gtcaggggtt ggttcagctc gtgtgccaat gacgaagcca
    10861 tttcccccat ggtggtgagg cggctggtga gctggatttt ttcctgctgg acgcgggacg
    10921 cttcttcgtg ccgccgccgt tcggtgatgt cgcgcgcgac ctgcaggcgc acccggcgac
    10981 catccgtcca ggccagaatg cgatggtgca cctcgaacca gcgttgcgac gactccgaga
    11041 acgcctcgat ggtttcatcg gtgaaccggc ccaggcgccc acccagcaat tccgcatgcc
    11101 cgcccgactg cgctccgaag aagcgtcggt acgtccggtt ggcgaacagg agctccaggc
    11161 catcggcggt gtctgccacc accgaaatcg cgtcgtccaa gccttccagc acagtcatga
    11221 aacgctcatg cgcggcggtg agggcttcac gaatgcgttt cggctcggtg atgtcggtca
    11281 tggatgtcat ccagccgatc tgttccccgt tgggatcgcg cagcggcgac acatacatgc
    11341 gcgctgtgaa gcgcgagccg tcgcggcgct gtgcctcgat ctcgagcccg ctgctgggcg
    11401 tgcggccgga taacagcagt tcaagggttt cctggtgttg cgcgtagcgg ccaggcaccc
    11461 agtacggaaa tggcgtggtg cgcccgatga ggtcggcctc attccagccg atcatgcgac
    11521 agaacgcggg attgacatag gcgatgcggc catgcatatc gaacacgcgc atgcccgtgg
    11581 acatggaatt ctccatggcg cggcggaagc cggtttcggc aatcagggcg gattccgcgc
    11641 ggctgcggaa gcgtgtgtag cgccagagcg cgagcaggct caggacgatc acgcacgaga
    11701 gtgcgacgac gaccagaagg aggcgctgct ggcgcgtttc ctggtcgatc gtcacgaaca
    11761 tcacgctgtc gttgtgcagc cgttgcagcc agatgaacac gcccatgacg cacaggtaca
    11821 gcaccaggac gatgaccggc gtcagccagt agaatccgcg ccgactgttg ttggcctgat
    11881 cgtagaacgt ggtcggaatg agggattttt tcgacggact cgccataggc tctataaata
    11941 aggcatagcg cattttagac cgtaaccggt gatcgtaaat tcatgggctg tgccttttgt
    12001 ccggttattt tccatattat aaaaattaat accgcaaagt gaaatttaac ttgctcaaca
    12061 cccagctctt tcctagaatg gctggagtga tgacgacgca ctggcgcggc cgcctggcca
    12121 acgattccgt gtgcgcaaaa gagcaagcgg ccccgtagtg gccgtcgagc cgtacccaac
    12181 acccagccaa ggagcaccca gatgtcttct cttgaaccca ccaccgccgt cgctgcggcc
    12241 gacgaagccc tggaaacaca ggaatggctt gaagcgctcg agtccgtgct cgatcgggag
    12301 gggccggagc gcgcccattt cttcatcgag aagctcatcg atctggcacg ccgatccggg
    12361 gcgcacattc cctattcgcc caacacggcc tacctgaaca ctattcctcc agggctcgag
    12421 cccgcgtatc cgggcaatct ggtgctcgaa gagcgtatcc gttcgtatat ccgctggaat
    12481 gccatggcga tggtcgtgaa ggccaaccgc cttcatccgg ccgacggtgg cgacctgggt
    12541 ggtcatatcg cgtcgttcgc ctcgctggcc accatggtgg cgtgtggcca gaatcacttc
    12601 tggcacgccg agacccctga tcacggcggc gatctggtct atttccaggg gcattcgtcg
    12661 cccggcatgt atgggcgcgc ctatctggaa ggccgtctca ccgaaggcca actcgacaac
    12721 ttccggcaag aagtcggcgg caaggggttg tcgtcctacc cccatcccaa gctgatgccg
    12781 gacttctggc agttctcgac cgtgtccatg gggctcgggc ccctgatggc catctaccag
    12841 gcccggttcc tcaagtacct gcatgcgcgc ggcatcgccg acaccagcaa gcgcaaggtg
    12901 tgggtgttct gcggcgatgg cgaaatggac gaacccgaat ccctgggggc cattggcctg
    12961 gcggcgcgcg aaaagctgga caacctcatt ttcgtcatca actgcaacct gcagcgcctc
    13021 gacggcccgg tccgtggcaa tggcaagatc atccaggagc tcgaaggtga cttccgcggt
    13081 agtggctgga acgtcatcaa actgatctgg ggcggatact gggatcccct gctggcgcgc
    13141 gacaaggaag gcatcctgcg gcgtgtcatg gaagagtcgg tcgatggtga ataccaggcc
    13201 tacaaggcgc gtgatggcaa gttcgtgcgc gaccatttct tcggcaaaca ccccaagctg
    13261 ctcgagatgg tcagccgcat gagcgacgaa gacgtctggc gcctgaatcg tggcggccac
    13321 gatccctaca aggtgtactc ggccttcgcc gccgccagtg cgcacaagga ccaacccacc
    13381 gtcattctcg ccaagaccat caagggctat ggcatgggcc aggtaggaca ggccaagaat
    13441 ccgacgcacc agcagaaaaa actggacacc gagtcggtcc gcgaattccg cgaccgcttc
    13501 aacattcc
//
LOCUS       DASTNK010000115         4603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1013464, whole genome shotgun sequence.
ACCESSION   DASTNK010000115 DASTNK010000000
VERSION     DASTNK010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013464"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VFR20_08440"
     CDS             <1..1009
                     /locus_tag="VFR20_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017685730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HEU0230587.1"
                     /translation="AMTLAALLLRDAYFRRPGHTKFKYMTIGVLFVNVSIGGVLTSFA
                     APPVLMVAHTFGWDAAHMATHFGWRAALAVVINAGVLTMLCRRYLIEREAAKAGAPSA
                     IASSGAGHDAAAGKVTREAGSAASVEAARVPRDPATGTVASPPTASASGKRARVPPLV
                     MGVHLAFLVGIVLGAHHPTVFLGLLMLFIGYTEAYRQFQSRLMIKEGLMVGFFLAGLV
                     VLGGMQHWWLQDLLAGLSPVVLYWGATALTAVTDNAALTYLGSLVSGTGELWRYMLVA
                     GAVTGGGLTVIANAPNPAGFAILKQYFPDGAISPLRLLGSALIPTLVAAFMFLLPNSF
                     T"
     gene            1266..1664
                     /locus_tag="VFR20_08445"
     CDS             1266..1664
                     /locus_tag="VFR20_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEU0230588.1"
                     /translation="MPIYAYKCSACGHTQDVLQKLSDPLLTVCPECGKSTYVKQLTAA
                     GFQLKGSGWYVTDFRDNGKKATDGAGAGDSHHAHTGHNGASHDGVSQNGSASGDSKPA
                     AAADTKAATSATPAPAKPAESSTGTSPSTK"
     gene            1693..2436
                     /locus_tag="VFR20_08450"
     CDS             1693..2436
                     /locus_tag="VFR20_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEU0230589.1"
                     /translation="MRFFKRYFVTGLLIWIPLVITVWVIALLVNTLESFVPRFLSPQS
                     LFGVHIPGFQLVLVLVVVLVTGLLGANFIGRALVDRWEKLLGRIPLVRSIYNSVKQVS
                     DTVLAPNGQAFREAVLVQYPRHGSWTIAFLTGVPGGEVANRLKGDYVSVYVPTTPNPT
                     SGFFLMMPRGDVQILDMSVDAALKYIVSMGVVVPGSGAAHDPAKAALLLENQPDASKK
                     HFHGGVGPHPSGKHTHGGEDPPADDAPGR"
     gene            2477..4270
                     /gene="aspS"
                     /locus_tag="VFR20_08455"
     CDS             2477..4270
                     /gene="aspS"
                     /locus_tag="VFR20_08455"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU0230590.1"
                     /translation="MRTCYTGEVGKTHLGQTVELFGWVHRRRDHGGVIFIDLRDRAGL
                     AQIVVDPDNAGAFAIAERIRNEFCVRISGLVRPRPEGTTNAELASGDVEVLCREIEIL
                     NPSVTPPFQLDDENLSETTRLTHRVLDLRRAPMQRNLMLRYRVAMEVRKYLDGLGFID
                     IETPMLTKSTPEGARDYLVPSRVNPGEFFALPQSPQLFKQMLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDCETSFLNEVEIRAVFEGMIRQVFATVQHVALPEQFPTMTWDE
                     AMRRYGSDKPDLRVKLEFTDVSDLMREVDFKVFSAPANDPASRVVALRVPGGAAMPRS
                     EIDAYTKFVAIYGAKGLAYIKVNDVASVPDGLQSPIVKNIHQAALEGLLQRTGAQSGD
                     LIFFGADKTRIVNDAMGALRVKIGHSEFGRQNGLFEAGWKPLWVIDFPMFEYDEQEKR
                     FFAAHHPFTSPKDGHEDFLESDPGRALAKAYDMVLNGWEVGGGSVRIHRAEVQSKVFR
                     ALNIDADEAAEKFGFLLDALQYGAPPHGGVAFGLDRLVTMMAGAESIRDVIAFPKTQR
                     AQCLLTHAPSEVDEKQLRELHIRLRNVEAAQ"
     gene            4446..>4603
                     /locus_tag="VFR20_08460"
     CDS             4446..>4603
                     /locus_tag="VFR20_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEU0230591.1"
                     /translation="MAIVRVVSYNIHKGRSALGNRESLSDLRLGLYGLRPDLLFLQEV
                     QGRNERHF"
BASE COUNT          790 a         1467 c         1470 g          876 t
ORIGIN      
        1 ggcgatgacg ctggcggcgt tgttgctgcg cgatgcctat ttcaggcgcc ccgggcacac
       61 gaaattcaaa tacatgacga tcggtgtgct gttcgtcaac gtgtccatcg gcggggtgct
      121 cacgtctttc gcggcaccgc cggtgttgat ggtcgcgcac acgttcggct gggatgccgc
      181 gcacatggcc acgcacttcg gctggcgggc ggcgctggcg gtggtgatca acgccggggt
      241 gttgacgatg ctctgccgtc gctacctgat tgaaagagag gcggccaagg ccggtgcacc
      301 ctcggccatt gcgtcttccg gggcgggcca tgacgcggcg gcaggcaaag tgaccaggga
      361 agccggcagc gcggcgtcgg tcgaggccgc acgggtgccg cgcgacccgg ccaccggcac
      421 tgtggcctcg ccgccaactg cgtccgccag cgggaagcgg gcgcgggtcc cgcccctggt
      481 catgggcgta cacctcgcct tcctggtggg catcgtgctg ggagcgcatc atcccacggt
      541 gtttctgggc ctgctcatgc tgttcatcgg gtacaccgag gcgtatcgcc agttccagag
      601 ccgtctgatg atcaaggagg ggctgatggt gggcttcttc ctggcggggc tggtcgtgct
      661 gggcggcatg cagcactggt ggctgcagga tttgttggcg gggctgtcgc ccgtggtgct
      721 gtactggggt gccacggcgc tgaccgccgt caccgacaac gccgccctca cctacctagg
      781 gtcgctggtg agcggcaccg gcgaattgtg gcgctatatg ctggtggccg gcgcggtcac
      841 gggaggcggc ctcaccgtca tcgccaacgc gcccaacccc gcggggttcg ccatcctgaa
      901 gcagtatttt ccagatggcg cgatttctcc gctgcggctg ttgggctccg cgctgatccc
      961 caccctggtg gcggcgttca tgttcctgct gcccaattcg ttcacgtgac atgggccgcc
     1021 gagcctggtt ccccaggccg gccattgccc ccacctcgcc gctggcgcgc gggaaatggg
     1081 tggaattcac caaaaacggc taaaatcacc tgttgcccac ttgattttcc aatgatcgat
     1141 accgcttttg cgtttgtccg gagcggtggc tcgggcttgc gccgcgaagt ttgtccgccc
     1201 ccctggcgaa gcggcctcct gcccgcatgg cgtgttgcgt ggacgaactt tcctgggatt
     1261 ttgttgtgcc tatctatgca tacaaatgca gcgcctgcgg ccatacgcag gacgtgctgc
     1321 aaaagctttc cgaccctttg ctcaccgtct gcccagagtg tggcaagagc acttatgtga
     1381 agcagctcac ggccgccggc tttcaactga aagggtctgg ctggtacgtg accgacttcc
     1441 gcgacaacgg caagaaggcg accgacggcg ccggtgccgg cgattcgcac cacgcgcaca
     1501 ctggccacaa tggcgcgagc cacgacgggg tgagccagaa cggctcggcg tcgggggatt
     1561 ccaaaccggc cgcggcggcc gataccaaag cagccacatc agccacaccg gctccggcca
     1621 agccggcgga atcgtccact ggcacctcgc cctcaacgaa ataagcaaga gtaaacaggc
     1681 cccgatcacg gcatgcgctt tttcaaacgc tactttgtca ccggactgct gatctggatc
     1741 ccactggtga tcaccgtatg ggtgatcgcc ctgctggtca acacgctgga aagcttcgtg
     1801 ccgcgattcc tgtcgccgca gtcgctgttc ggtgtgcaca tccccggttt ccagctggtg
     1861 ctggtgctcg tcgtggtgtt ggtgacgggc ctgctggggg cgaatttcat cggccgggcc
     1921 ctggtcgatc gttgggaaaa gctgctcggc cgcataccgc tggtgcgctc catctacaac
     1981 tcggtcaagc aggtgagcga caccgtgctg gcgcccaatg gccaggcgtt tcgcgaggcc
     2041 gtgctggtgc agtacccccg tcatggttcc tggaccatcg cgttcctcac gggcgtgccg
     2101 ggcggcgagg tcgccaatcg gctcaaaggc gattacgtca gtgtctacgt gccgaccacg
     2161 cccaacccca catcaggttt ctttctgatg atgccgcgtg gcgatgtcca gatactcgac
     2221 atgagtgtcg acgcggcgct gaaatacatc gtgtccatgg gtgtcgtggt gccgggatcg
     2281 ggcgccgccc atgacccggc gaaagcggct ttgttgctcg agaatcaacc agacgcctca
     2341 aagaagcatt ttcatggcgg ggtcgggccc cacccttcgg ggaaacacac acacggcggg
     2401 gaagatcccc ccgctgacga tgcgccgggg cgttgacccc gcgtttgcaa agatatcttt
     2461 aacggagtga ttctgcatgc gtacctgcta taccggcgag gttggtaaaa cccacctggg
     2521 ccagacagtc gaactgttcg gctgggtgca tcgccgtcgt gaccacggtg gtgtcatttt
     2581 catcgacctg cgcgatcgcg caggtctggc ccagatcgtc gtggatcccg acaacgccgg
     2641 ggctttcgcc atcgcggagc gcattcgcaa cgaattttgc gtgcggatct ccggcctggt
     2701 gcgcccgcgc cccgaaggca cgaccaatgc cgaactcgct tccggcgatg tcgaggtgct
     2761 gtgccgcgag atcgagatcc tcaacccgtc ggtcaccccc ccgttccagc tcgacgatga
     2821 aaacctgtcc gaaaccacgc gcttgacgca tcgtgtgctg gacctgcgcc gcgcgcccat
     2881 gcagcgcaac ctgatgctgc gctaccgggt ggccatggaa gtgcgcaagt acctcgacgg
     2941 cctggggttc atcgatatcg aaacccccat gctcaccaaa agcacgcccg aaggcgcgcg
     3001 tgactacctg gttccatcac gcgtgaaccc gggcgaattc ttcgcgctgc cccagtcgcc
     3061 gcaactgttc aagcagatgc tgatggtcgc cggcttcgac cgctactatc agatcaccaa
     3121 atgcttccgc gatgaagacc tgcgcgccga tcgccagccc gagttcaccc agatcgactg
     3181 cgaaacatcc ttcctgaacg aagtcgagat ccgcgcggta ttcgaaggca tgatccgcca
     3241 ggtgttcgcc acggttcagc acgtcgcgtt gccggaacaa ttccccacca tgacgtggga
     3301 cgaggccatg cgccgttatg gttccgacaa gcccgacctg cgcgtcaagc tcgaattcac
     3361 cgacgtgtcg gatctcatgc gcgaagtgga tttcaaggtg ttctcggcgc cggccaatga
     3421 cccggccagc cgtgtcgtgg cgctgcgcgt gcccggcggc gcggccatgc cccgtagcga
     3481 aatcgacgcc tataccaagt tcgtggcgat ctacggcgcc aagggcctgg cgtacatcaa
     3541 ggtcaacgat gtggcgtccg tcccggatgg cctgcagtcg cccatcgtca aaaacatcca
     3601 ccaggccgcc ctggaaggac tgcttcaacg caccggcgcc cagtcgggtg acctgatttt
     3661 cttcggcgcc gacaagacgc gcatcgtgaa cgacgcgatg ggcgcgttgc gcgtgaagat
     3721 cggacacagc gagttcggcc ggcagaatgg cctgtttgaa gccggctgga agccgttgtg
     3781 ggtcatcgac ttccccatgt tcgagtacga cgaacaggag aagcggtttt tcgcggcgca
     3841 tcacccattc accagcccca aggacgggca cgaggatttc ctcgaatcgg atcccggccg
     3901 cgcgttggcc aaggcctacg acatggtgct caatggctgg gaagtcggcg gcgggtcggt
     3961 gcgtatccat cgcgccgagg tgcagagcaa ggtgttccgg gcgctgaaca tcgacgccga
     4021 cgaggcggcc gaaaaattcg gcttcctgct ggatgccctg cagtacggcg ccccgccgca
     4081 tggcggggtg gccttcggcc tggatcgcct ggtgaccatg atggccgggg ccgagtccat
     4141 acgcgatgtc atcgccttcc cgaagacgca gcgcgcgcag tgcctgctca cgcatgcgcc
     4201 gtcggaagtc gacgagaagc agctgcgcga gctgcacatt cgcctgcgca acgtcgaagc
     4261 ggcccagtga cttcggggcc gtcgccggtt ggcggtctct ccaatatccg cgatgctgac
     4321 cgggctgggt gtttttcagg cttggcattc gtgcccggag tagtacgata ggacaagcgt
     4381 ggcggccgcc gcgttcggcg gttcgaacgc ggtgcggtcg cgcccgtatt ccacaagagg
     4441 cgcatgtggc aatcgttcga gtcgtcagct acaacattca caagggccgt tcggcgctgg
     4501 gcaatcggga gtccctgtcc gatctgcgcc tgggcctgta cggtctgcgt ccagacctgc
     4561 tgttcctgca ggaagtgcaa ggccgcaacg aacgccattt cag
//
LOCUS       DASTNK010000116        29924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_459829, whole genome shotgun sequence.
ACCESSION   DASTNK010000116 DASTNK010000000
VERSION     DASTNK010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 29924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29924
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459829"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /locus_tag="VFR20_08465"
     CDS             <1..117
                     /locus_tag="VFR20_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0230592.1"
                     /translation="LSDRIMVLHHGEKIADGQPDEVLANSTVIEAYLGKKRV"
     gene            114..851
                     /locus_tag="VFR20_08470"
     CDS             114..851
                     /locus_tag="VFR20_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0230593.1"
                     /translation="MTSASPNRSPLLKMAGVSAGYGEMTVLRDISMEIYPSEIVAMVG
                     SNGAGKTTMLRALSRLIDSTGETTFEGKSLTGLHPDQVFELGMVQVPEGRQLFDRMSV
                     QDNMLMGAHRRTHASRAEIQRDLDDMYALFPRMAERRKQLAGSMSGGEQQMCAMARAM
                     MAKPKLLMVDEMSLGLAPIIVDQLMDILVDIRGKGVTVLLVEQDIHLALATADRGYVI
                     ETGSIVQQGPSADLINDPALQKAYLGL"
     gene            complement(855..1808)
                     /locus_tag="VFR20_08475"
     CDS             complement(855..1808)
                     /locus_tag="VFR20_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEU0230594.1"
                     /translation="MNLQQFRFVRETIRRNFNLTDAARTLFTSQPGVSKAIIEFEDEL
                     GVKIFERHGKRIKGLTKPGVVVAQVIDRIMREVDNLKKVSDEFARRDEGGLIIACTHT
                     QARYLLPKVIPAFRERFPKVHVSLAEGNPQQLANMVLHEQADLAIATETLSMTTGLVS
                     LPVYSWQHTVVVRPDHPLADLSSSEARDLSIEQLAQYPLVTYDRAFSGRCAIDQKFSD
                     AGLDPDIVLEAIDADVVKTYVDIGLGVGIIAGVAFDPRRDKGLVGIPVGHLFGTHVTC
                     AAIKSGVFLRDYVYVLLEMLAADLTRDVVTRALEEKKVLAA"
     gene            complement(2038..3531)
                     /locus_tag="VFR20_08480"
     CDS             complement(2038..3531)
                     /locus_tag="VFR20_08480"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEU0230595.1"
                     /translation="MSDVPRVPLLIGGELVQSKTTDWRDVLNPATQEVVAKVPFATKE
                     ELALAVSNAKEAFKTWRNTSQAVRVRVMLNLQALVRENLTKIAELVTLEHGKTLPDAE
                     GEVGRGLEAIEHACSLNSLQMGEYAENAARGIDVYSICQPLGVCAGITAFNFPIMLPA
                     WMFPIAVTAGNTFVLKPSEQDPRSSLMLAQLALEAGLPPGVLNVIHGGPDIANGLCDH
                     PDIKAISFIGSTHVGTEIFRRATGQGKRCQAGMGAKNHCIVLPDADRDAALNQLLGAG
                     FGAAGQRCMATSVAIFVGEAKEWVPDLVTRARKLKVNAGSDREADLGPLVSPAARKRV
                     EGLIQKGVDEGADLLLDGRNVKVPGYGEGNFVGPTIFGGVKTDMTVYQEEIFGPVLDV
                     ICVDTLEEAIELVNENPNGNGVGLFTQDGGAARYFQNNIDVGNVGINIPIPVPVAWFS
                     FTGWRASKWGALGPNGKEAVKFWTQIKTVTARWSAHNVNVNTTITQH"
     gene            complement(3562..4746)
                     /locus_tag="VFR20_08485"
     CDS             complement(3562..4746)
                     /locus_tag="VFR20_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0230596.1"
                     /translation="MALCLGLSAGAQAKVGPVKIGVLTDMSGPYSAMGGQGSVVAAQM
                     AIDDCLKHQCKGMKISLVSADNQNKADVGAAKAREWIDRDGVTALADLTNSAVALAVQ
                     DIAKQKGIVAMFSGPATTELTNSHCSPTGFHWMFDTYSQSVGGVKAVVKKGGKSWYFI
                     TVDYAFGHSLQKNAGAMVKSLGGTVVGSVQYPLNTTDYASYLLKAQSSKAQVIALANA
                     GQDVVNAVKQAREFGIAKGGQKLVALLVFSTDLKGMGPKDAQGLTYVDGFYWNQDDAA
                     RKWSARFAKAYKNRKPTMVQAGVYSSVLHYLEAVAASGSTDGKVVAAEMRKLPIVDPI
                     MHHASILPNGRVIHDMYLYQAKSPAESTGPWDLAKLVATIPAKDAFQPLSESTCPLLK
                     KQ"
     gene            4993..6036
                     /locus_tag="VFR20_08490"
     CDS             4993..6036
                     /locus_tag="VFR20_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0230597.1"
                     /translation="MGTIPASGNSSIRGTTAKPLALDWDGLRFFLEVARAQRVSAAAL
                     RLGVRHTTVSRRIRALEHELGTLLFDKSKSSGFTLTEDGLRLFTYAEQIEGTLVSARE
                     NLSGHMQTPAGHLRVGSTEGFGSYVLTPLMTDFQRRYPNITLDIMPVPRFISLSKREA
                     DIAISLERPQRGPYLCTKLIDYSLKLYGSVDYLAHAPRIDVMADLAHHGFIGYVEELL
                     FSDRLRYLEDIVPAGRVVFKSTSVIAQYHAALQGNALAILPCFIAAQDPRLRVVLDDQ
                     IKVTRSFWMYCHEDLRGSKRVMALWDFIKQAVPLNAALLRGESDQLRYLPVRDAPETD
                     PSASRRASAAEGP"
     gene            complement(5996..7111)
                     /gene="lptF"
                     /locus_tag="VFR20_08495"
     CDS             complement(5996..7111)
                     /gene="lptF"
                     /locus_tag="VFR20_08495"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEU0230598.1"
                     /translation="MSLFKRSVVSEIMSHAGVVFSTLLVVWLSVLLVRLLGQAANGDI
                     GASVVFGLAAFSSITALPVILAVSLFIAVLTTVTRNFRESEMVVWFASGISLRDWVGP
                     VLRCAVPVAAMVAVLTLFASPWASRQIVEYKQRYQQRSDVSKISAGQFIETNDGARVF
                     FANSPGRPGDDLGRIIARVIGPEWLSIFTASSVRVEQEPNGDRFLVLGPGRRYDIKPG
                     KSDFRLFDFQKYGFRLERKGNVSAEEAQARAEREMRARPTVQLFHDNNRHALGQIMWR
                     VSLPLAALNLALLAIPLGSVNPRLGRSGDLLIAGLVGLLYMNLMNLSQRWIESNSLSF
                     WEGIGLVHGSFFVLMMYLMWRKLRVKAPRPPRPAATR"
     gene            7177..8685
                     /locus_tag="VFR20_08500"
     CDS             7177..8685
                     /locus_tag="VFR20_08500"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEU0230599.1"
                     /translation="MEFSTLTTASLHQIKTAALAVGVFADGVLTPAADIIDHSSNGSI
                     RSVLKTEFSAKPNTHLVLRNLPGVSAARVVLIGLGKQDAYNARAHAGAELAFARYCVS
                     ARLTEAVSTLVAIECAGSTIQRRAQAAAVAAGQATYHYDATLGSKDADAAPRLKKLAL
                     AVARNQASQAQQGLLHGRATAGGMALARELGDLPANVCTPTYLGAAAKKLAREFKQIK
                     VEVLERKQIEALKMGSFLSVARGSYEPPVFIILKYTPTAGKTTKKAGKAPIVLVGKGI
                     TFDAGGISIKPAAKMDEMKYDMCGAASVLGTLRAVAEIGLDREVIGLVPSCENLPSGR
                     ANKPGDVVTSMSGRTIEILNTDAEGRLILCDALTYAERFKPAAVIDMATLTGACIVAL
                     GHANSGLFSPDDELAESLLQAGREANDPAWRLPVEDAYQDLLRSNFADVANIGGPAAG
                     AVTAACFLSRFTGAYRWAHLDIAGTAWRSGGSDKGATGRPVALLTQYLMNQA"
     gene            8710..9189
                     /locus_tag="VFR20_08505"
     CDS             8710..9189
                     /locus_tag="VFR20_08505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEU0230600.1"
                     /translation="MAHVDFAFGAPNRLRMACDVARKHYLAGHRLLVYCRDETVLARF
                     DRLLWGFEPTAFVPHVRTGDPLEADTPVVLASSLPLPGATPESTVGAPGDDAPWLLNL
                     DPEVPPRVEQFARVLEIVSSHDADKQAARQRWRHYESSGHTLRAFDVSPPPESDTPP"
     gene            9452..10162
                     /locus_tag="VFR20_08510"
     CDS             9452..10162
                     /locus_tag="VFR20_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU0230601.1"
                     /translation="MSDFRPTSLPSRSDYTASPGEVARNRVLRNTYWLLTLSLIPTVL
                     GAVVGLRTGINHAMSANPGTSAIIFLLGAFGLMFLIERNKNSSVGVALLLGFTFFMGL
                     MLSRMLGFVMGMSNGAQLIMLAFGGTAVVFGAMASIASTTKRDFSHWQKFLFIGVVLL
                     IVASLANIFLQLPALMLTISVLAIAIFSVFLLVDLQRVINGGETNYVTATLAIYLDLY
                     NVFVNLLMLLGVLGGNRN"
     gene            complement(10197..11120)
                     /gene="rarD"
                     /locus_tag="VFR20_08515"
     CDS             complement(10197..11120)
                     /gene="rarD"
                     /locus_tag="VFR20_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEU0230602.1"
                     /translation="MKLGIGLALSSSVLFATLYYYSTLLSPLGGEDIFAWRILLGMPA
                     LAILISRSRGWGEARGLWVALRADPKLFVVLLTGAALIGVQLWLFMWAPLHQKALDVS
                     LGYFLLPLTMVLAGSLFYHERLGALQWAAVALAAVGVAHELIRTASFSWATALVMFGY
                     PPYFMLRRRLRYGSLLMLWVDVLLLVPAALAMLIFHDGVVAPFLAHPRLLLLVPLMGV
                     LSSAALACYISASRLLPMSLFGLLGYMEPVLLFWVALLLLGEGMAPGALWTYGPIWGA
                     VGLIALQGVRSWLAERNAAAGQKKAAESSRL"
     gene            complement(11117..11731)
                     /locus_tag="VFR20_08520"
     CDS             complement(11117..11731)
                     /locus_tag="VFR20_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEU0230603.1"
                     /translation="MAALDLPGLVVLDTCVLLSGRLRHVLLGLSAHGCFQPIWSPVIG
                     DEWRRNASRAAGVPVEQAGIWWDEWERDFPAASQPDIASFKDGLKHSDPKDWHVIAAG
                     RAALNSQPGASVAILTRNIRDFSRSELRRMGLGLFDPDAFLVRCLDRHPVVLTRLLNE
                     LTQETAVWSAEPLPTILRRERLFRLNRLLALPEPGEPDGKTETR"
     gene            complement(11781..12368)
                     /gene="recO"
                     /locus_tag="VFR20_08525"
     CDS             complement(11781..12368)
                     /gene="recO"
                     /locus_tag="VFR20_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU0230604.1"
                     /translation="MSRQPQRVHEAVGYLLHATPWRETSLVIQMFTRDHGSVALVAKG
                     AKRPYSTLRPVLMAFQPLSLHWSGAGEVKTLSRAECNGIRPLAGRALMSAWYMNELLL
                     RLLPREDPHPVLYDAYDAALSDLSQGSSAAGALRRFEWILLRETGYGLDQEAPDFADA
                     GLEPVLRGSLRERLDELLAGRPLQTRKVLMDLQRY"
     gene            complement(12361..13245)
                     /gene="era"
                     /locus_tag="VFR20_08530"
     CDS             complement(12361..13245)
                     /gene="era"
                     /locus_tag="VFR20_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU0230605.1"
                     /translation="MASFHCGFVAIVGRPNVGKSTLANALIGGKVSIVSRKAQTTRHR
                     IHGVLTRDDAQFVFVDTPGFQTRHGGAMNRMMNRVVTQALADVDVVAHVVEAGKWSSG
                     DEQLLPLLPSGDKSILVVNKVDLLKNKNDMFPFVGKIMARHAYGAVVPVSASRGVQLE
                     QLLGELRNRLPEGEAMFDADTLTDRPMRFVAAELLREKIFRLVGDELPYGCTVMIEQW
                     EEDDKTARVAACILIERESHRPILLGAGGAHMKRIASEARQDIAALLDKRVFLEVYIK
                     VRRGWSDHEGALRELGYE"
     gene            complement(13245..14009)
                     /gene="rnc"
                     /locus_tag="VFR20_08535"
     CDS             complement(13245..14009)
                     /gene="rnc"
                     /locus_tag="VFR20_08535"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU0230606.1"
                     /translation="MTSHASLEQALGYQFRDASLLERALTHRSHSAQHNERLEFLGDS
                     VLNFVVASLLFRRFTKIDEGDLSRVRASLVKQASLAEIAQRLVLSQHLRLGEGELKSG
                     GFRRPSILADALEAVFGAVFLDSGFDAVQKVIADQYAPMLAGIDPKTLGKDAKTLLQE
                     VLQGRKYELPVYRVVATHGAAHNQMFEVECAIPALEITVSAPGASRRAAEQSAATLAL
                     AALRAISPAKGAKSRPRAPRKSTQLSLPVAVTQESK"
     gene            complement(14009..14896)
                     /gene="lepB"
                     /locus_tag="VFR20_08540"
     CDS             complement(14009..14896)
                     /gene="lepB"
                     /locus_tag="VFR20_08540"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU0230607.1"
                     /translation="MSWDFALILFILLVLTGAVWCVDVFSLRKRRRAAGEAAALACEY
                     SVAGMAPADAEKIRNEAHAKASRPSWFIEYCVSFFPVILFVFVLRSFVVEPFRIPSGS
                     MLPTLENGDLILVNKFQYGIRLPIVDKKIISLGTPHRGDVMVFRYPLDPSVDYIKRVI
                     GLPGDTILYQNKTLYINGKEITHVRDGDFFEPDRSTYVGRYTEQLGNVKHSILLDKYA
                     PQNFMPIADYPYRKNCEYLDKGVRCTVPPNHYFMMGDNRDNSLDSRYWGFVPDANIVG
                     RAFFIWMNFSHPSRIGTFH"
     gene            complement(14919..16715)
                     /gene="lepA"
                     /locus_tag="VFR20_08545"
     CDS             complement(14919..16715)
                     /gene="lepA"
                     /locus_tag="VFR20_08545"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEU0230608.1"
                     /translation="MDHIRNFSIIAHIDHGKSTLADRLIQRCGGLADREMSSQVLDSM
                     DIERERGITIKAQTASLNYRAADGRVYAFNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIELGVEVVPILNKMDLPSADPDTARSEIEDVIGIDATNAIL
                     ASAKTGQGIDEILEAIVARVPAPKGDPDAPLQALIIDSWFDNYVGVVMLVRIVNGRLR
                     PKDKILLMASGVTHLCEQVGVFTPKSEQRKELSAGDVGFVIAGVKQIDDAKVGDTITL
                     AGQPAPEPLAGFKEAKPQVFAGLYPVESSEYDQLRDSLEKLKLNDSSLMFEPEVSQAL
                     GFGFRCGFLGLLHMEIVQERLEREFDMDIITTAPSVVYEVLHRDGSLLMIESPSRMPE
                     VGGIVEIREPIVTVTLFMPQEYVGPVMTLCMAKRGIQLDMSYHGRQVHLVYEIPLAEI
                     VLDFFDKLKSVSRGYASMDYEFKEYRAADVVRVDLLINGDRVDALSMIVHRANARYRG
                     REVVSKMRGLIPRQMFDIAIQAAIGAEIIARESIKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKKRMKQVGSVEIPQEAFLAILQVEDKS"
     gene            complement(16833..18323)
                     /locus_tag="VFR20_08550"
     CDS             complement(16833..18323)
                     /locus_tag="VFR20_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEU0230609.1"
                     /translation="MLSTSMADSRLKRIIASVSTGVLLAFSVAGAPSYAETAPAPAPV
                     LSLPDFTSVVAKTEGSVVNIRTTEAVPVRSPGPDSDDPYNMFRWFFGPNFLPPGMQPP
                     RQHHRSPEAKPRERTVPRGVGSGFFISSDGYILTNNHVIAHSNGIYVTLTNGKEYKAK
                     IIGTDPRTDIALIKIDIHGAPPLTIGDSDKLKKGQWVLAIGSPFGLDSTVTAGIVSAI
                     NRDTGDYLPFIQTDVAVNPGNSGGPLINLSGQVVGINSQIISESGGFMGISLAIPIDE
                     VMRVVHQLKAHGKVTRGRIGVQIGQVSDDVAKALGLDRAKGAMVSNVESSSPASEAGV
                     QPGDVIIKFNGSNIAHWTDLPRLVGITKPGTRVPMEVWRKGKVVTLSVKVGELTDQKA
                     NASQTPAQPAAPVVDALGLKVTSVPEAERTKDKIKGGVKVVESADPAASAGINTGDVI
                     LTVNNVDVTGPDQFAKLVGKLNKSHAAALLVLRGNQTQWITVTPEK"
     gene            complement(18349..19347)
                     /locus_tag="VFR20_08555"
     CDS             complement(18349..19347)
                     /locus_tag="VFR20_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HEU0230610.1"
                     /translation="MQLSRICRWLAVAACLVAQPVLAAGPAPSAVDPTLALLQKIQGA
                     ARQLDYAGVFTYQQGPVLQSTRIVHMVDGTGERERLEMLDGAPGEFLRHNDNIERLVP
                     EHKIVVLETLRADRFPGLLITDGRNIPAYYSVIKNKRPHRVAGRECVMIELMPKDHDR
                     YGHRYCSDTKTDLLLKSQTLGPGGGVIDQISFTNLKVGESVQPSQLEPHWNTKGWKVL
                     EAPMRPIDLAKMGWRISVPAGFVDVTQVQRPMKAGKQVNQLVLSDGLAAISVFIEPFD
                     KAHEQALPKGAVRKGALNIYGTRIGDHWFTVVGEVPVRTLRDIAKRTEYVPLASSR"
     gene            complement(19389..19880)
                     /locus_tag="VFR20_08560"
     CDS             complement(19389..19880)
                     /locus_tag="VFR20_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HEU0230611.1"
                     /translation="MNTASHSKDVPEPASWEASVSAWVDGEESIRSEELNLPYGRHVW
                     DTYHLIGDVMRSGELGIQPSERFYARVSQAIDKEPPIVAPHARRRLPVRLGVSGLVVA
                     AAVASVVWVALPYFSGSGPAQVQVVASASDNPGLTDYLDAHSQLIGAGPVRQVSFDTG
                     VVR"
     gene            complement(19892..20491)
                     /gene="rpoE"
                     /locus_tag="VFR20_08565"
     CDS             complement(19892..20491)
                     /gene="rpoE"
                     /locus_tag="VFR20_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HEU0230612.1"
                     /translation="MSEREVDAELVARVQRGDKRAFDLLVLKYQRKIMRLLSRMIRDP
                     SEIEDVAQEAFVKAYRALPQFRGESAFYTWLYRIAINTARNWQVANGRRPSTPTTIEN
                     EDGETFSQIDNLTDISTPESMLASRQVVETVNAAIDALPEDLKTAIVLREIEGLSYEE
                     IAESMGCPIGTVRSRIFRAREAIAAQLRPILDTDTERRW"
     gene            complement(20488..20997)
                     /locus_tag="VFR20_08570"
     CDS             complement(20488..20997)
                     /locus_tag="VFR20_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HEU0230613.1"
                     /translation="MRHGTVRSVRPGIPDVGAPVVLSWPVSRPRWVALLEPGLLAAAM
                     AGAAYCLASPYVLTMGLSLWWVRERSRRIRAEASSGVLSLGADDRWQWAPSDGARMAH
                     LCPVRVWLGPFWISLRFVDQDRLVSKDAMLQVTLWKSRVPPQAWRRLQVCLASRIARP
                     AIVAAQGGQ"
     gene            complement(21014..22246)
                     /gene="fabF"
                     /locus_tag="VFR20_08575"
     CDS             complement(21014..22246)
                     /gene="fabF"
                     /locus_tag="VFR20_08575"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU0230614.1"
                     /translation="MKRRVVITGLGIVSPIGNDVESAWDNVVNGRSGIGRITRFDASA
                     LNSQIAGEVKGFDVTQYLSAKEARQMDTFIHYGVAAGLQAWRDSGLDLSDSDGERAGV
                     IMGSGIGGLPRIEETQIETLQRGLKRISPFFVPGALINLISGQLSILLGLKGPSYAVV
                     SACTTGLHSIGDAARLIEYGDADVMLAGGAESTVSPLGIGGFAAMRALSMRNDDPATA
                     SRPFDRDRDGFVLGEGAGALMLEEYEHARKRGARIYGEFAGYGMSSDAHHITAPDRDG
                     PRRGVLNALRNGGVNPDEVDYVNAHGTSTPLGDINETEALKLAFGDHARKLVVNSTKS
                     MTGHLLGAAGGIEAVFTTLAVYRQVSPPTINIFSQDPECDLDYCANQARDMKIDVALS
                     NSFGFGGTNGSMIVRRLS"
     gene            complement(22568..22807)
                     /gene="acpP"
                     /locus_tag="VFR20_08580"
     CDS             complement(22568..22807)
                     /gene="acpP"
                     /locus_tag="VFR20_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU0230615.1"
                     /translation="MESIEQRVKKIVAEQLGVNDAEIKNESSFLDDLGADSLDMVELV
                     MALEDEFETEIPDEEAEKITTVQQAVDYITSHSKQ"
     gene            complement(22998..23741)
                     /gene="fabG"
                     /locus_tag="VFR20_08585"
     CDS             complement(22998..23741)
                     /gene="fabG"
                     /locus_tag="VFR20_08585"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEU0230616.1"
                     /translation="MQERHLEGKTALVTGATRGIGQAIVRELALRGARVIGTATSRTG
                     ADAISATLAGAGGRGVVLDVTDAAACDALVAELGKEDGGPHILVNNAGITRDNLAMRM
                     KDDDWDAVINTNLTAVFRLSRAVMRSMMKARWGRIINITSVIGFSGNPGQANYAAAKA
                     GVTSMTRSLARELGSRNVTVNCVAPGFIDTDMTRALSDAQRESILAQVPLGRLGLPTD
                     IANAAAFLASPQADYITGTTVHVNGGLYM"
     gene            complement(23746..24678)
                     /gene="fabD"
                     /locus_tag="VFR20_08590"
     CDS             complement(23746..24678)
                     /gene="fabD"
                     /locus_tag="VFR20_08590"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU0230617.1"
                     /translation="MKIAFVFPGQGSQSVGMLDAWAGNTVVAEALGLASAALGQDLAS
                     LIAGGPAEQLNLTTNTQPAMLASAVAMYRAWLAAGGPEPSLVAGHSLGEYSALTAARS
                     LALEDAVRIVRVRADAMQTAVPVGTGGMAAILGLDDDVVRDVCAQAARGEMVEAVNFN
                     APAQVVIAGHATAVARACELAKAAGAKRALPLPVSAPFHSRLLEPAATVLGKALAGVS
                     MAAPGIELINNVDVASPSDPAAIRDALVRQAWHPVRWVETIRAMKDRGITHVIECGPG
                     KVLSGLTRRIDKDLQSFNVSDPASLEATLAVIKE"
     gene            complement(24766..25740)
                     /locus_tag="VFR20_08595"
     CDS             complement(24766..25740)
                     /locus_tag="VFR20_08595"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEU0230618.1"
                     /translation="MPYAKIIGSGSYLPPRVVSNDELAADLATRQIETSDTWITERTG
                     IRQRHIAEPGVKTSSLAINAGRAALEHAGVTATDLDLIIVATSTPDFIFPSTACLVQA
                     GLGASGGAAFDVQAVCSGFVYAMITADSFIRSGRARTALVIGAEVFSGILDWNDRGTC
                     VLFGDGAGAVVLRASDEPGISGAKLGADGRQAGILCTAGGISRGKVEGDPFLRMDGQA
                     VFKLAVTVLAQSARDVCEQAGVALSDVDWMIPHQANVRILNMLGRKLGIPTEKIVITL
                     DRHANTSAASVPLAFDVAFRDGRIRAGQQVLMQGVGGGFTWGSLLVRI"
     gene            complement(25765..26847)
                     /gene="plsX"
                     /locus_tag="VFR20_08600"
     CDS             complement(25765..26847)
                     /gene="plsX"
                     /locus_tag="VFR20_08600"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU0230619.1"
                     /translation="MSKTRIRIAVDCMGGDIGLPVTIPAAHRFAQEHPDTRFLLVGDA
                     QAIQAGIRKIGVRQDDWYEILPASEVVSMEDSVEVALRRKKDSSMRVAAQAVKDGAAD
                     ACISAGNTGAWMAISRYVLKTLDGIDRPAIATSIPNQKGGATTMLDLGANVDCTADHL
                     LQFAIMGTALVKTVDLHDNPTVGLLNIGEEVIKGNEVVKQAADLLRASGLNFYGNVEG
                     DDICKGTVDVVVCDGFVGNVVLKSIEGVAKMVASMLRQEFTRDALSMTAGMLSKPVLN
                     RLRDRVDNRRYNGAALRGLRGIVIKSHGSADVYAFGYALQRAHEAVLNGLLDGTMNAM
                     DHLRQRLQDAAASSAKPTNDLSNPVS"
     gene            complement(26879..27061)
                     /gene="rpmF"
                     /locus_tag="VFR20_08605"
     CDS             complement(26879..27061)
                     /gene="rpmF"
                     /locus_tag="VFR20_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU0230620.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDFLGAPATAVEPTTGELHLRHHISP
                     NGVYRGRKVIQTKNDD"
     gene            complement(27116..27640)
                     /locus_tag="VFR20_08610"
     CDS             complement(27116..27640)
                     /locus_tag="VFR20_08610"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HEU0230621.1"
                     /translation="MSAVRYIDTYELARSGSAVAGVVSLRDFGRLCAGLPEQADQSVE
                     WSVRADKNTGGEVFLWVSVKTAVLLECQRCLEAFAQPLDVSTRLQVVAEADLDDGGSH
                     DPDGNGVAVERIAASHRLDILELIEDEIILGLPYVPRHDACPSESASPADSESDEKRP
                     SPFSVLGKLKKPTL"
     gene            complement(27850..29181)
                     /locus_tag="VFR20_08615"
     CDS             complement(27850..29181)
                     /locus_tag="VFR20_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEU0230622.1"
                     /translation="MSQSYFPGWRRIQSTGAGLVVQPDECLSWPQNVAMGAQHVVAMF
                     GSTILAPLLMGFDPNVAILMSGIGTLIFFLFVGGRVPSYLGSSFAFIGGVITVTGYAG
                     AGHNVNIPLALGAIIVCGVVYAVIGIIVWWVNARAGDAAGWIDALMPPAVTGAIVAVI
                     GLNLAPIAAKGAMGGTNFEAIMALVTVLCVGGIAVYTRGTTQRLLILVGLLLACVLYA
                     VCANGLGLGRPMDFSAVAAAPWLGLPHFEAPVFSTHAIGLIAPVAVILVAENLGHIKA
                     VSAMTGRNLDIYLGRAFLGDAVATMVSGSVGGTGVTTYAENIGVMTVTRIYSTLVFVI
                     AGLIAIVLGFSPKFGALIHTIPGPVLGGMSIVVFGLIAVAGARIWVVNQVDFADNRNL
                     IVTAVTLILGAGNFSLQFGDFKLDGIGTATFGSIILYALMGLGRRRATSVG"
     gene            29331..>29924
                     /locus_tag="VFR20_08620"
     CDS             29331..>29924
                     /locus_tag="VFR20_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HEU0230623.1"
                     /translation="MKLIQTTRANLTRPNPDPMRLILASSSRYRRDMLARLGLPFDTE
                     APGVDETPQNGEEPESLARRLSLAKALAVASRHPGAVIIGSDQVASLEGAPIGKPGGF
                     DRAREQLRRLAGRTVTFHSGLCVTDGLHHEVVCVDTFCRFRPLKDQDIDTYLRREQPF
                     DTAGSAKAEGLGVALMERMTSDDPTAIIGLPLIELCRL"
BASE COUNT         5949 a         9685 c         8903 g         5387 t
ORIGIN      
        1 ctgtcggacc gcatcatggt gctgcaccac ggcgaaaaaa tcgccgatgg acagcccgac
       61 gaagtgctgg ccaattccac agtgatcgaa gcgtatctag ggaagaaaag agtatgacca
      121 gcgcaagccc caaccggagc cccctgttga agatggcggg tgtgtcggcc ggatacggcg
      181 aaatgaccgt gctgcgtgac atcagcatgg aaatctaccc gtccgagatc gtcgccatgg
      241 tcggcagcaa cggcgccggc aagacaacca tgctgcgggc gctgtcgcgg ttgatcgaca
      301 gcaccggcga gaccaccttc gaaggcaagt cgctgaccgg actgcatccc gaccaggtgt
      361 tcgagctggg catggtccag gtgcccgaag gccgccagtt gttcgaccgc atgtcggtgc
      421 aggacaacat gctgatgggc gcgcaccgcc gcacccatgc cagccgcgcc gaaatccagc
      481 gcgacctgga cgacatgtac gccctcttcc cccgcatggc ggagcgccgc aagcaactgg
      541 cgggcagcat gtcgggtggc gagcagcaga tgtgcgccat ggcgcgcgcc atgatggcga
      601 agcccaagct gctgatggtc gacgaaatga gcctgggcct ggcccccatc atcgtcgacc
      661 agttgatgga catcctggtg gacatccgcg gcaagggcgt gacggttctg ctcgtggaac
      721 aggacatcca cctggcgctg gccaccgccg atcgcggcta cgtgatcgaa accgggtcga
      781 tcgtgcagca aggcccgtcg gcggatctga tcaacgaccc ggcgttgcag aaggcgtatc
      841 tgggtctctg aggctcaggc ggccaggacc ttcttctctt ccagggcccg cgtgaccaca
      901 tcgcgggtca ggtcggccgc cagcatttcc agcagcacat agacgtaatc acgcaggaac
      961 acgcccgact tgatcgcggc gcaggtgacg tgcgtgccga acaggtggcc gacaggaatg
     1021 ccgaccagcc ccttgtcgcg acgcgggtcg aacgcgacac cggcgatgat gcccacgccc
     1081 aggccaatgt cgacataggt cttgaccacg tcggcgtcga tggcctccag aacgatgtca
     1141 gggtcgagtc cagcgtcgga aaatttctgg tcgatggcgc agcgacccga gaacgcgcgg
     1201 tcgtaagtga ccagcgggta ctgggcaagc tgctcgatgg agagatcccg ggcttcactg
     1261 gaggacaggt ccgccaatgg atgatcgggg cgcaccacaa cggtgtgctg ccaagaatag
     1321 actggcaggc tgaccaggcc cgtggtcatg gacagcgttt ctgtggcgat ggcgaggtcg
     1381 gcctgctcat gcagcaccat gttggccagt tgctgcggat tgccttcggc cagtgacaca
     1441 tgcactttgg gaaatctttc gcggaaggcg ggaatgactt ttggcagcag atagcgcgcc
     1501 tgcgtatggg tgcaggcgat gatcaacccc ccttcgtcgc gccgggcgaa ttcatcgctg
     1561 accttcttca ggttgtcgac ctcgcgcatg atgcgatcaa tgacctgcgc caccacgacg
     1621 ccgggcttgg tcagcccctt gatgcgcttg ccgtggcgct cgaagatctt gacgcccaac
     1681 tcatcctcga actcgataat ggccttggac acgccgggtt gcgatgtgaa cagggttcgt
     1741 gcggcatccg tcaggttgaa gttccggcgg atggtttcac ggacgaatcg gaattgctgc
     1801 aggttcatga aatggccctt actggaaaag ccattatatg taataaagaa ataaaataat
     1861 attatttata gttatatgtt taagggcgct cgggacaaat caaacggcaa actgacgcag
     1921 tggatcggga tcggatgcaa gacgcaaaaa aaggccccgg ccagaaacac cccggaaggt
     1981 cggatccgtg tccaactttc cggagtcggt tcaaaacccg gggcgcctca cctcgactca
     2041 gtgctgggtg atggtcgtgt tcacgttcac gttatgcgcc gaccagcgcg ccgtcacggt
     2101 cttgatctgc gtccagaact tcacggcttc cttgccgttt ggccccagcg caccccactt
     2161 cgacgcgcgc cagccggtga agctgaacca ggccaccggc acggggatgg gaatgttgat
     2221 gcccacgttg cccacgtcga tgttgttctg gaagtagcgc gccgcgccgc cgtcctgcgt
     2281 gaacaggccc acgccgttgc cgttggggtt ctcgttgacc agctcgatgg cttcctcgag
     2341 tgtgtcgacg cagatgacgt cgagcactgg cccgaagatc tcttcctggt agacggtcat
     2401 gtcggtcttg accccgccga agatggtcgg gcccacgaag ttgccctcgc cataacccgg
     2461 gaccttgacg ttgcgaccgt cgagcaagag gtctgcaccc tcgtccacgc ccttctggat
     2521 gagcccttcg acacgcttgc gcgccgctgg cgacaccagc gggcccagat cggcttcgcg
     2581 gtctgatccg gcattgacct tgagcttgcg cgcacgcgtg accagatcgg gcacccactc
     2641 tttggcctcg cccacgaaga tggccacgga cgtcgccatg caccgctggc ccgccgcgcc
     2701 aaagcccgcg cccagaagct ggttcagggc ggcgtcgcgg tcggcgtcgg gcagcacgat
     2761 gcagtggttc ttcgcaccca tccccgcctg gcagcgtttg ccctggcccg tggcgcgccg
     2821 gaaaatctcg gtgcccacgt gcgtggagcc gatgaacgag atggccttga tgtcagggtg
     2881 gtcgcacaag ccgttggcga tgtccgggcc gccatggatg acgttcagca caccaggagg
     2941 caggccggct tccagcgcca gttgcgccag catcaacgac gagcggggat cttgctcgga
     3001 cggcttcaga acgaaggtgt tgcccgccgt cacggcgatg gggaacatcc aggccggcag
     3061 catgataggg aagttgaacg cggtgatgcc ggcacacacg ccaagcggct ggcaaatgct
     3121 gtagacgtcg atgccgcgcg ccgcgttttc agcgtattcg cccatctgca acgaattcag
     3181 cgagcaggca tgctcgatgg cctcgaggcc gcgaccgacc tcgccctcgg cgtcgggcag
     3241 cgtcttgccg tgttcgagcg tgacgagctc ggcgatcttg gtcaggttct cgcgcaccag
     3301 cgcctgcaaa ttgagcatga cgcgcacgcg caccgcctgg ctggtgttgc gccaggtctt
     3361 gaaggcttcc ttggcattgg agaccgccag ggccagctct tccttggtgg cgaaaggcac
     3421 ttttgcgacc acttcctggg tggctggatt gagcacgtcg cgccagtcgg tggttttcga
     3481 ctgcacgagc tcgccgccga tcagtagcgg tacgcgtggc acatcactca tgaatatctc
     3541 ctgggaatgt tgatggatga atcattgttt tttcaacagc gggcaggtcg actcggacaa
     3601 gggctggaac gcgtccttgg ccggtatcgt cgctaccagc ttggccagat cccaaggccc
     3661 tgtcgattca gcgggagact tggcctggta caaatacata tcgtgaatca cacgaccgtt
     3721 gggcaggatg gaggcgtgat gcatgatcgg gtccacaatg ggcagtttac gcatctcggc
     3781 ggccacgacc ttgccgtcgg tcgagcccga cgccgccacg gcttccagat agtgtagaac
     3841 actagaatac acccccgcct gcaccatggt tggcttgcga ttcttgtagg ctttggcgaa
     3901 acgtgccgac catttccgcg cggcatcgtc ctgattccaa tagaagccat cgacatacgt
     3961 cagcccctgg gcatccttcg ggcccatacc tttcaggtcg gtcgagaaca ccagcaatgc
     4021 aaccagcttc tggccgccct tggcgatgcc gaattcccgc gcctgcttga cggcgttaac
     4081 gacgtcctgt ccggcgttgg ccaaggcgat cacctgcgcc ttggagctct gcgccttcag
     4141 cagatacgag gcatagtctg tggtgttgag cggatactgc accgacccca cgaccgtgcc
     4201 acccagactc ttgaccatgg cgccggcgtt cttctgcagg gaatggccaa aggcatagtc
     4261 caccgtgatg aaataccagg acttgcctcc cttcttcacc accgccttga ccccacccac
     4321 cgactgggaa taagtgtcga acatccagtg gaatccggtg ggggagcaat gcgagttcgt
     4381 gagttcggtc gtggccgggc ccgagaacat ggcaacgatg cctttctgct tggcgatgtc
     4441 ctgcaccgcc agcgccacgg cggagttggt caggtcagcc agcgcggtca caccatcacg
     4501 atcgatccac tcgcgggcct tggccgcgcc cacgtcagcc ttgttctggt tgtcggccga
     4561 caccagcgat atcttcatgc ctttgcactg gtgtttgagg cagtcgtcta tggccatttg
     4621 cgcggccacc accgagccct gcccgcccat ggcggaatag gggccggaca tatccgtcag
     4681 aacgccaatc ttgaccggcc cgaccttggc ctgcgcccca gcagacaaac ccaggcaaag
     4741 ggccatcccc agcgtcaatg cgaatttcct catgtctcgt ctccgtttgt ttttgcttga
     4801 cttcatgatg ccggggcaat ccggcccacg cgccgggtag gggctcgggc cgggttaccc
     4861 cggaaacccg tcaaaggcca cgggtcgcgc cgcatccccg ctccccttcc ccgggttctt
     4921 tgcagtgtag ttgtgtaaaa aacaagattc caccacaata tttgcacatt ccttgttcat
     4981 atttgcacaa cgatgggcac catacccgcc agcggcaatt cctccatacg cggtaccacc
     5041 gcgaaacccc tcgctctcga ctgggacggc ttgcgatttt tcctggaagt cgcgcgtgcg
     5101 cagcgggtca gtgccgccgc gctgcgactg ggcgtgcggc acaccacggt gtcgcggcgc
     5161 atccgggcgc tggagcacga actgggcacg cttctgttcg acaagtccaa atcgtccggc
     5221 ttcacgctca cggaagatgg cctccgcctg ttcacctatg cggagcagat cgaaggcacc
     5281 ctggtttccg cgcgggaaaa tctgtccggc cacatgcaga cgcccgccgg gcatttgcgc
     5341 gtcggatcga ccgagggatt cggcagttac gtgctgaccc cgctcatgac cgacttccag
     5401 cggcgctacc cgaacatcac gctggacatc atgccagtgc cccgtttcat cagtctatcg
     5461 aaacgggagg ccgacatcgc catctcgctt gagcggccgc aacgtggccc gtacctgtgc
     5521 accaagctca tcgactattc attgaagctg tacggcagtg tcgactatct cgcccacgcg
     5581 ccgcgcatcg acgtcatggc cgatctggcg catcacggtt tcattggtta cgtggaggaa
     5641 ctattgttca gtgaccgcct gcgctacctg gaagacatcg tgcccgccgg ccgcgtggta
     5701 ttcaagagca ccagtgtgat cgcgcagtat catgcggcgc tgcaggggaa tgccctggcc
     5761 atcctgccgt gcttcatcgc ggcccaggat ccccgcctgc gcgtcgtgct ggatgatcag
     5821 atcaaggtga cccgcagctt ctggatgtac tgccacgaag acctgcgcgg ctcgaaacgg
     5881 gtcatggccc tgtgggattt catcaaacag gccgtgccgc tgaacgcggc cctgttgcgc
     5941 ggggagtccg accaattacg ctacctgccc gtccgggacg cccccgaaac ggaccctagc
     6001 gcgtcgcggc gggcctcggc ggccgagggg ccttgactct taacttgcgc cacatcaggt
     6061 acatcatcaa gacgaagaaa gagccatgca ccaagccgat cccttcccag aaggaaaggc
     6121 tgttcgactc gatccatcgt tgcgacagat tcatcaaatt catgtacagc agccccacca
     6181 gtcccgcaat gagcaaatcc ccggatcgcc ccagccgtgg attgacggaa cccagcggga
     6241 tggccagcaa ggccagattc agggcggcca gtggcaacga cacgcgccac atgatctgcc
     6301 ccagagcgtg ccgattgttg tcatggaaca attgcaccgt cggccgcgcc ctcatttcac
     6361 gctcggcccg tgcctgggct tcttcggcag acacgttgcc cttgcgctcg agacggaacc
     6421 cgtatttctg gaaatcgaac aggcgaaaat cggactttcc cggcttgatg tcatagcggc
     6481 gtcccggccc cagcaccaga aaacggtccc cgttgggctc ttgttcgacc cgcacgctgc
     6541 tggcggtgaa tatgctgagc cattcggggc cgatcacacg ggcgatgatc cgcccaagat
     6601 cgtcgcccgg gcggcccgga ctattggcga agaacacccg tgcgccatcg ttcgtctcaa
     6661 tgaactggcc ggcgctgatc ttggacacgt cggagcgctg ctggtagcgc tgcttgtact
     6721 ccacgatctg gcgggacgcc cagggcgacg cgaacaaggt caacaccgcc accatcgccg
     6781 cgaccggcac cgcgcaacgc agcacgggcc ccacccagtc ccgcaacgat atgccgctgg
     6841 cgaaccagac caccatctcc gactcccgga aattacgagt gaccgtggtc aacaccgcga
     6901 tgaacagcga caccgcaagg atgaccggca acgcggtgat gctggaaaac gccgccaggc
     6961 cgaacaccac actcgcacca atatcgccgt tggcggcttg ccccaacaga cgcaccaaca
     7021 gaacgctcag ccagaccacc agcagcgtgg agaaaaccac ccccgcatgg ctcatgattt
     7081 cggatacgac agaacgtttg aataaagaca tgagaaaata agcgggacaa tcgatggcat
     7141 ccgacgcgtt cggcagacac ctaacgggaa actcgcatgg aatttagcac actgaccacg
     7201 gcttcactgc atcaaatcaa gacagcggca ctcgccgtcg gtgttttcgc ggacggcgtc
     7261 ctgacacccg ccgccgacat tatcgaccac tccagcaacg ggtcgatccg cagcgtactg
     7321 aagacggaat tctccgcgaa gccgaacacc catctggtgc tccgaaacct tcccggcgtc
     7381 agtgccgccc gcgttgtgct gatcggtctg ggcaagcaag acgcatacaa cgcccgggcg
     7441 catgccggcg ccgaactggc gttcgcccgc tactgcgtct cggcacgact cacggaagct
     7501 gtgtccacgc tggtcgccat tgaatgcgcc ggctcgacca tacagcggcg cgcccaagcg
     7561 gcagccgtgg cagccggcca agccacttac cattatgacg caaccctggg cagcaaagac
     7621 gccgatgccg cgccccggct caagaaactg gcgctggcgg tggcgcgcaa ccaggcatcc
     7681 caggcccagc aaggcctgct tcacggccgc gccacagctg gcggcatggc tctcgcccgc
     7741 gaactgggcg accttcccgc caacgtctgc acccccacct acctgggcgc tgccgcgaaa
     7801 aaactggcgc gcgaattcaa acaaatcaag gtcgaggtgc tcgaacgcaa acagatcgaa
     7861 gccttgaaaa tgggttcatt cctgtcggtg gcccggggct cgtacgaacc acccgtgttc
     7921 atcatcttga aatacactcc gacggcgggg aaaaccacca aaaaggctgg caaggcgccc
     7981 atcgttctcg tcggcaaagg catcacgttc gatgccggcg gcatttccat caagccggcc
     8041 gccaaaatgg acgaaatgaa gtatgacatg tgcggcgccg ccagcgtgct gggcacgctg
     8101 cgggcggtcg cggaaatcgg cctggatcgc gaagtgatcg ggctggtgcc ctcctgcgag
     8161 aacctgccca gcgggcgcgc gaacaagccg ggcgacgtcg tcaccagcat gtcgggccga
     8221 accatcgaaa tcctcaacac cgatgccgag gggcggctca tcctgtgcga cgcgctcacc
     8281 tacgccgagc gcttcaagcc cgcggccgtc atcgacatgg ccacgctcac gggcgcctgc
     8341 atcgtcgccc tgggccacgc caattccggc ctgttctctc ccgatgacga actggcggaa
     8401 agcctgcttc aagcgggacg cgaagccaat gaccccgcct ggcgtctccc ggtggaagat
     8461 gcctaccagg atcttctgcg ctcgaatttc gccgatgtgg ccaacatcgg cggcccggcg
     8521 gccggcgcgg tcacggcggc ctgctttctc tcgcgcttca ccggcgccta ccgctgggcg
     8581 cacctggaca tcgccggcac ggcctggcgc agcggcggca gcgacaaggg cgccaccggg
     8641 cgcccggtgg ccctgctgac ccaatatctg atgaatcagg cctgacgcgg cgctccggtg
     8701 tctcgaccta tggctcacgt cgatttcgcc ttcggggcac cgaaccgcct gcgcatggct
     8761 tgcgacgtcg cgcgcaaaca ctacctggcg ggccatcggc tgctggtgta ctgccgggac
     8821 gaaacagtcc tggctcgttt cgaccgtctg ctgtgggggt tcgaacccac cgcgttcgtg
     8881 ccccatgtgc ggacgggcga tccactcgaa gccgacacac ccgtggtgct ggcgtcctcc
     8941 ctgcccttgc ccggcgcgac cccggaatcc accgttggcg cacccgggga cgatgccccc
     9001 tggctactca atctcgatcc cgaagtgccg ccacgggttg aacaattcgc ccgcgtactg
     9061 gaaatcgtct ccagccacga cgccgacaaa caggccgccc gccagcgctg gcgccattac
     9121 gaatcaagcg gccacaccct gcgggcgttc gacgtatcgc caccgcccga atccgatacg
     9181 ccgccctgag gagcaatgcc cctggagcgg accaagtacg gcgccgccga gtgtctggaa
     9241 ccaaacaccc gcgcaccgat ccaatccatg tcgcctgatt ccgcttgtgc attcagataa
     9301 atgtaaggta tcatcgagtc gagagctgaa ccggcgaata ggcacgcgct atactgagaa
     9361 ccgacgtgcg tggcgatgtg gattcaccgc tgccacagcg atcatcgccg ccacctggcg
     9421 gcatggcaac acaacctgaa aggacccgac aatgagcgat ttccgcccca cttctcttcc
     9481 gagccgcagc gactataccg cttcgcccgg ggaagtcgca cgcaaccgtg tcttgcgcaa
     9541 cacgtactgg ctgctcacgc tgtcactgat acccacggtg ctgggcgcgg tggtgggcct
     9601 gcgcaccggc atcaaccatg ccatgagcgc caaccccggc acatcggcca tcatcttcct
     9661 gctgggcgcg ttcggcctca tgttcctgat cgagcgcaac aagaacagca gtgtcggcgt
     9721 cgccctgctg ctgggcttca cgttcttcat gggcctgatg ctctcgcgca tgctgggctt
     9781 tgtcatgggc atgagcaacg gcgcacagct catcatgctg gccttcggcg gcacggctgt
     9841 cgtcttcggc gccatggcgt ccatcgccag caccaccaag cgcgacttct cccattggca
     9901 aaaattcctg ttcattggcg tggtgctgct catcgtcgcc tcgctggcga acatcttcct
     9961 gcagctgcca gccctgatgc tcacgatttc cgtgttggcc atcgccatct tctcggtgtt
    10021 tctgctggtc gacctgcaac gcgtcatcaa tggcggtgaa accaactacg tcaccgccac
    10081 ccttgccatt tatctggacc tgtacaacgt tttcgtcaac ctgctgatgc tgctgggcgt
    10141 gcttgggggc aatcgcaact agtaccccct catgcccgac cctttgccgg gtcactctac
    10201 aagcggctgc tttcggccgc ttttttttgc cccgccgcag cgttccgctc ggccaaccag
    10261 cttcgcaccc cctgcaacgc gatcaggccc accgcccccc agatcgggcc gtacgtccac
    10321 agcgccccgg gcgccatgcc ctcgccgagc agcagcaacg ccacccagaa caacagcacc
    10381 ggctccatgt acccgagtag tccgaacaga ctcatgggca gcaggcggct ggcggagata
    10441 tagcatgcca acgcggccga gctcagcacc cccatgagag gcaccagcag caacagcctg
    10501 ggatgcgcca gaaacggtgc gaccacgcca tcatggaata tgagcatcgc aagcgcggcc
    10561 ggcacgagca gaagcacatc cacccaaagc atcagtaacg acccataacg caagcgtcgc
    10621 cgcagcatga aatagggcgg gtagccgaac atgaccagcg cggtcgccca ggaaaacgac
    10681 gccgtgcgaa tgagttcgtg cgcgacgccc accgccgcca gcgccaccgc ggcccactgc
    10741 aaggctccca gccgctcgtg gtagaacagg ctcccagcca ggaccattgt caacggcagc
    10801 aggaaatacc ccagggaaac atccaatgcc ttctgatgca gtggcgccca catgaataac
    10861 caaagctgga cgccgatgag cgccgccccc gtcaacaaga cgacgaacag cttcggatcg
    10921 gcgcgcagcg ccacccagag accgcgcgcc tcgccccagc cccgggaacg ggaaatcaga
    10981 atggccagcg ccggcatccc cagcaatatg cgccaggcga aaatatcctc ccccccaagc
    11041 ggggacaaca gagtcgaata atagtaaagc gtcgcgaaca gcaccgacga ggacaacgcc
    11101 agcccaatac cgagcttcac ctcgtctccg tcttaccgtc aggctcgccc ggctcaggca
    11161 aagcaagcag gcggttgagt cgaaacaacc gttcgcgtct aagaatggtg ggcaggggct
    11221 cggcggacca gacagcggtt tcctgtgtca gctcattcag aagccgcgtc agaacgacgg
    11281 gatgccggtc aaggcaccgc accaggaaag catccgggtc aaacagcccc agccccatgc
    11341 gccgcagctc ggagcgactg aaatcacgga tattgcgagt cagaatggcc accgacgcgc
    11401 caggctggct gttcaatgcc gcacgccccg ccgcgatcac atgccagtct ttcgggtcgc
    11461 tgtgtttcag accgtccttg aatgacgcga tatccggctg gctcgccgct ggaaaatccc
    11521 gctcccactc atcccaccag attccggcct gctccaccgg aacgcccgcg gcacgggagg
    11581 cgttgcgccg ccattcgtca ccgatcaccg ggctccagat gggttgaaag caaccgtgtg
    11641 cgctcaagcc cagcaacaca tgccgcaacc gtccggacag cagcacacaa gtgtcgagca
    11701 cgacaaggcc cggcaggtcg agggccgcca tcaggacatc ttcctcgcca tgcgaggcgc
    11761 attgtcagaa tgattcacgc tcaataccgc tgcagatcca tgagcacctt gcgcgtctgc
    11821 aagggacgac cggccagcag ttcgtcgagc cgctcacgca atgaaccgcg cagcaccggt
    11881 tccaatccgg catcggcgaa atcgggcgct tcctgatcca ggccgtagcc cgtttccctg
    11941 agcaatatcc attcaaagcg ccgcagggcg cccgctgcgg aggacccttg cgagaggtca
    12001 ctcaatgccg cgtcataggc gtcgtagagc acggggtggg gatcttcgcg cggcagaagc
    12061 cgcaacaaca gctcattcat gtaccaggcc gacatcagcg cccgcccagc cagaggccgg
    12121 atgccgttgc attccgcgcg cgacaatgtc ttgacctcgc ccgccccgct ccagtgcagg
    12181 ctaagaggct gaaacgccat cagcaccggc cgcaaagtgg aataaggccg cttggccccc
    12241 ttggccacca gcgcgacact gccgtggtca cgcgtgaaca tctgaatgac cagcgaggtt
    12301 tcgcgccatg gcgtggcatg cagcaggtag cccacggcct cgtgcacgcg ctgtggctgt
    12361 ctactcatag ccgagttcgc gcaaggcccc ctcgtgatcc gaccaacccc tgcgcacctt
    12421 gatatagact tccaggaaca cgcgcttgtc gagcaatgca gcgatgtcct gccgtgcctc
    12481 ggaggcaatg cgtttcatgt gggcgccgcc cgctcccagc agaatggggc ggtggctttc
    12541 ccgttcgatg agaatgcacg cggccacgcg cgcggtcttg tcgtcttctt cccattgttc
    12601 gatcatcacg gtgcagccat acggcagctc atcccccacc aggcggaata tcttctcgcg
    12661 cagcaactcg gcggccacga accgcatggg ccgatcggtg agcgtatcgg catcgaacat
    12721 ggcctcgccc tcaggcaaac ggttgcgcag ttcaccaagc aattgttcga gctgcacgcc
    12781 gcgcgacgcg ctgaccggca cgacggcacc gtaggcatgg cgcgccatga ttttcccgac
    12841 aaacggaaac atgtcgttct tgttcttgag caggtcgact ttgttgacca ccagaatgga
    12901 tttgtcgccg gaaggaagca atggcaggag ctgttcgtcg cccgacgacc atttccccgc
    12961 ctcgaccaca tgcgctacga cgtcgacatc ggccagtgcc tgggtcacga cccggttcat
    13021 catgcggttc atggcgccgc cgtgccgcgt ctggaagccc ggcgtgtcga cgaacacgaa
    13081 ctgggcatcg tcgcgcgtga gcacaccatg gatgcggtgg cgggtcgtct gcgccttgcg
    13141 cgaaacaatg gataccttgc ctccgattag cgcgttggcg agtgtcgatt tgccgacatt
    13201 gggccggccc acgatggcca cgaagccgca atggaaagac gccattattt agattcctgg
    13261 gtgacggcca ccggcagcga tagctgggtg gacttgcgcg gcgccctggg ccggcttttg
    13321 gcgcccttgg ctggcgaaat ggccctgagc gccgccagag ccagcgtggc ggccgactgt
    13381 tcagccgcgc gccggctcgc gcctggcgcg ctgacggtga tctcgagcgc cgggatggcg
    13441 cattcgacct cgaacatctg gttatgcgcg gcgccatgcg tcgccacgac ccgatacacc
    13501 ggaagctcgt acttgcgtcc ctggaggact tcctgcaaca gggtcttggc atccttgccc
    13561 agggttttcg gatctatgcc tgccaacatg ggggcgtact gatcggcgat gaccttctgc
    13621 acggcgtcga atcctgaatc caggaaaaca gcaccgaaga ccgcctcgag cgcgtccgcc
    13681 agaatggacg gccggcggaa gcccccgctt ttgagttcac cttcgcccaa ccgcaagtgc
    13741 tgcgacagca ccagccgctg ggcgatctcg gcgagcgacg cctgcttgac cagactggcc
    13801 cgcacgcgcg agagatcacc ctcgtcgatc ttggtgaatc gccggaacag cagggacgcc
    13861 accacgaaat tcagcacgga gtcgcccagg aactccagcc gttcgttgtg ctgcgcacta
    13921 tggctgcgat gcgtgagcgc ccgttccagc agggacgcgt cacggaactg atagcccagt
    13981 gcctgttcca gggatgcgtg actggtcatc aatgaaacgt acctatgcgg cttggatggc
    14041 tgaagttcat ccagatgaaa aacgcccgcc cgacgatgtt cgcatccggc acgaaccccc
    14101 agtaacggct atcaaggctg ttgtcgcgat tgtcacccat catgaagtaa tgattcggcg
    14161 gcaccgtgca gcgcacccct ttgtccagat actcgcagtt cttgcgatag ggataatcgg
    14221 caatgggcat gaaattctgt ggagcgtact tgtcgagcag gatgctgtgt ttgacgtttc
    14281 ccagttgttc agtatagcgc cccacgtagg tggaccggtc cggctcgaaa aagtccccgt
    14341 cgcggacatg ggtgatttcc ttgccattga tgtacaaggt tttattctga tagaggatcg
    14401 tgtcgccggg cagaccgatg acgcgcttga tgtaatcgac gctgggatcc aacggatagc
    14461 ggaaaaccat gacgtcgccc cgatgtgggg tacccaggct gatgattttc ttgtcgacga
    14521 tgggcaggcg tatgccgtac tggaatttat tgaccaggat caggtcgcca ttctcgagcg
    14581 tgggtagcat ggagcctgat ggaatgcgaa agggctcgac cacgaacgaa cgcagcacga
    14641 acacgaacag gatcactgga aaaaagctga cgcagtattc gatgaaccac gatgggcggc
    14701 tggccttggc gtgggcctca ttgcggattt tctcggcgtc ggccggcgcc atacccgcca
    14761 ccgagtactc gcaagccagc gcggcggctt cgccggcggc ccggcgtcgc ttgcgcaggc
    14821 tgaacacgtc cacacaccag acggcgccgg taagcaccag cagaatgaac aaaatcagtg
    14881 cgaaatccca actcatgctt gtgatacctt cttgaatact acgatttgtc ttccacctgc
    14941 aggatcgcca ggaaagcctc ctggggaatc tcgacactcc cgacctgttt catgcgcttc
    15001 ttgcccgcct tctgcttttc cagcagcttt ttcttgcgtg tgatgtcacc accgtaacac
    15061 ttggccagca cgttcttgcg caacgccttg atgctctcgc gggcgatgat ctcggcgccg
    15121 atggccgcct ggatggcgat gtcgaacatc tggcgaggga tcagaccgcg catcttcgag
    15181 accacctcgc gcccgcggta ccgtgcgttg gcgcggtgca cgatcatcga caacgcgtcg
    15241 acgcggtcgc cattgatgag caggtcgacc cggaccacgt cggcggcacg gtattccttg
    15301 aattcgtaat ccatcgacgc ataaccccgc gacacggatt tcagcttgtc gaagaaatcg
    15361 agcacgatct cggcaagcgg gatctcatac accagatgaa cctggcgccc gtggtaggac
    15421 atgtccagct gtatgccgcg cttggccatg cacaatgtca tgacggggcc gacatactcc
    15481 tgcggcatga acagtgtcac cgtcacgatg ggctcgcgga tctcgacgat gccgccaacc
    15541 tcgggcatac gcgaggggct ttctatcatc aacaacgagc cgtcgcgatg cagcacctcg
    15601 tagaccacgg acggcgccgt ggtgatgatg tccatgtcga actcgcgctc cagacgctcc
    15661 tgcacgatct ccatgtgcag cagccccagg aacccacaac ggaaaccgaa tcccagggcc
    15721 tgggagactt ccggctcgaa catgagcgat gaatcgttga gcttcagttt ttccagggag
    15781 tcgcgcagct gatcatattc gctgctttcg acgggataca ggccggcgaa gacctggggt
    15841 ttggcctctt tgaatcccgc caggggttcg ggagccggct ggccggccag cgtgatggtg
    15901 tcacccactt tcgcgtcatc gatttgcttg acaccggcga tgacgaaacc cacatcaccc
    15961 gctgacagct ccttgcgctg ttcggacttg ggcgtgaaca cccccacctg ctcgcacaga
    16021 tgcgtcaccc cgctggccat caggaggatc ttgtccttgg gtcggagccg gccattcacg
    16081 atgcgcacca gcatcaccac accgacgtaa ttgtcgaacc aggaatcgat gatcagcgcc
    16141 tgcagcggcg catcagggtc gcccttggga gctggcacgc gcgccacgat ggcttccagg
    16201 atctcatcga tgccctggcc ggttttggcg ctggccagaa tggcattggt ggcgtcgatg
    16261 ccgatcacgt cttcgatttc gctgcgagcc gtatccgggt cggccgaagg cagatccatc
    16321 ttgttcagga tgggaaccac ttcgacaccc agctcgatgg ccgtgtagca attggccacg
    16381 gtctgtgcct cgaccccctg cgacgcatcg acaaccagca acgccccctc gcacgcagag
    16441 agcgaacgac tgacctcgta ggaaaaatcc acgtgtcccg gggtatcgat gagattgaac
    16501 gcgtagaccc ggccatcggc cgcccggtag ttcaacgagg cggtttgcgc cttgatggtg
    16561 atgccccgct cacgctcgat atccatggaa tccagcactt gcgaagacat ctcgcgatcc
    16621 gccaaccccc cgcagcgttg tatcagccga tcggccagcg tggacttgcc atggtcgatg
    16681 tgggcgatga tggaaaaatt gcggatgtga tccataaatc aaggtcgtga atacggcgga
    16741 gggggatgag ggtatgcccg gtggcaaaaa aaggagcgca cagggcgctc cttttttgcg
    16801 gctgatccag cattttagcc gttttgtcgc gattatttct caggcgtgac cgttatccac
    16861 tgggtctggt tgccgcgcaa taccagcaac gcggccgcat gggacttgtt gagcttgccg
    16921 accagcttgg cgaactgatc aggtcccgtc acgtcgacat tattgaccgt caggatgaca
    16981 tcgccggtgt tgatgccggc cgatgccgcc ggatcggcgg attcaaccac tttcacgccg
    17041 cccttgatct tgtctttggt gcgttcggct tcaggcacgc tggtgacctt cagccccagg
    17101 gcatccacca cgggtgccgc tggctgggcc ggcgtctggg aggcgttcgc tttctgatcc
    17161 gtgagctcgc cgaccttcac actgagggtg accaccttgc ctttgcgcca gacttccatg
    17221 ggaacccggg tgccaggctt ggtgatgccg accaggcgtg ggagatccgt ccagtgcgcg
    17281 atgttggagc cattgaactt gatgatcacg tcgccaggct gcacgccggc ctcagaggcg
    17341 ggagagctcg attcaacgtt gctgaccatc gcgcccttgg cccgatcaag ccccagcgcc
    17401 ttggccacgt catcgctcac ctgcccgatc tgcacgccga tacggccgcg cgtcaccttg
    17461 ccgtgcgctt tgagctgatg caccacgcgc atgacttcat cgatggggat ggccagggaa
    17521 atccccatga acccaccgct ttcggagatg atctgggaat tgatccccac cacttgaccg
    17581 gacagattga tcagcggccc gcccgaattg ccgggattga ccgcgacatc ggtctggatg
    17641 aatggcagat aatcgccggt gtcacggttg atcgcactga cgatcccggc cgtgacggtg
    17701 gaatccaggc cgaacggcga gccaatggcc agcacccact gccctttctt gagcttgtcg
    17761 gaatcaccga tggtcaacgg aggggcgcca tggatgtcga ttttgatcag cgcgatgtcg
    17821 gtgcgcggat cggtgccaat gatcttggcc ttgtattcct tgccattggt cagcgtcacg
    17881 tagatgccat tggaatgcgc gatcacgtgg ttgttggtca ggatgtagcc atcgctggaa
    17941 atgaagaacc ccgaccccac accgcgcgga accgtgcgtt cgcggggctt ggcctctggc
    18001 gaacgatggt gctggcgcgg cggctgcatg ccgggcggca ggaaattggg accgaagaac
    18061 cagcggaaca tgttgtacgg gtcatcgctg tcgggtcctg gcgagcgcac cggcacggct
    18121 tcggtggtgc ggatgttgac cactgaccct tccgtcttgg cgacgaccga ggtgaaatca
    18181 ggcagggaca gtacgggcgc gggcgcgggt gcggtttcgg cgtatgacgg tgcccctgcg
    18241 acggaaaacg ccagcagaac cccagtcgac accgacgcga ttattcgttt gagtctgcta
    18301 tcggccatac ttgttgagag catcatggaa ctcctgtgaa aagccaaact acctggatga
    18361 cgccaaagga acatattcgg tacgcttggc gatgtcgcgc aaagttcgca caggcacctc
    18421 gcccacgacc gtgaaccagt gatccccgat tcgggttccg tatatgttga gcgcgccttt
    18481 tcggacagcg cccttcggca acgcttgctc atgggccttg tcgaacggtt cgatgaaaac
    18541 cgatatggcg gccaagccat cggacaagac caactgattg acctgcttgc ccgctttcat
    18601 gggccgctga acttgggtga cgtcgacgaa acccgccggc accgaaatgc gccagcccat
    18661 tttcgccaga tcgatcggcc gcatcggcgc ctccagcacc ttccacccct tggtgttcca
    18721 gtgcggttcg agctggctgg gctgaacgct ttcgcccacc ttcaggttgg tgaaggaaat
    18781 ctgatcgatc actccaccac cgggacccaa cgtctgggac ttgagcagca agtcggtctt
    18841 cgtatcggaa caataccgat gcccataacg atcatgatct ttgggcatga gctcgatcat
    18901 gacgcactcg cggccagcca cgcgatgcgg ccgcttgttt tttatgacac tgtaatatgc
    18961 gggaatgttc cgtccgtcgg tgatcaacaa ccccggaaaa cggtcggcgc gaagggtttc
    19021 aagcacaacg atcttgtgtt caggaacgag gcgctcgata ttgtcgttgt gtcgcaggaa
    19081 ttcgcccggc gcgccatcga gcatttcaag acgctcacgc tcaccggtgc cgtcgaccat
    19141 atgcacgatg cgggtcgact gcagaactgg accttgctga taggtgaaca caccggcata
    19201 gtccaactgg cgcgcggcgc cctggatttt ctgcagcaat gcgagcgttg gatcgaccgc
    19261 ggacggtgcc ggcccggccg ccagaacagg ctgtgccacc aggcatgccg ccaccgccag
    19321 ccaacggcag atccggctca gttgcaccgg gcgctctgcg cgccaactgg ttgcgacatg
    19381 ccgcgccatt acctgaccac ccccgtatcg aacgacacct gccgcacggg ccccgcgcca
    19441 atgagttggg aatgggcgtc gaggtagtcg gtcaggccag gattgtcact tgcgctggcg
    19501 accacctgaa cctgcgcggg gcccgaaccc gagaaatatg gcagtgccac ccagacgacc
    19561 gatgccaccg ccgcggccac caccaacccg gacacgccga gacgcaccgg taggcggcgg
    19621 cgcgcatgag gcgccacgat gggaggttct ttgtcgatgg cctgggatac ccgcgcatag
    19681 aatcgttcag agggctggat accgagctcc ccgctacgca tcacgtcgcc gatgaggtgg
    19741 taggtatccc agacatggcg cccgtatggc aagttgagct cttcggaacg tattgattcc
    19801 tcgccgtcga cccatgcgga taccgacgcc tcccaggaag cgggttcggg aacatccttg
    19861 gaatgagagg ctgtattcat cgaatgactc cttaccatcg acgctcggta tcggtgtcca
    19921 gaattggacg caattgtgcg gcgatggcct cacgtgcgcg aaatattcgg gaacgcaccg
    19981 taccaatggg gcatcccatg ctctccgcta tttcttcata gctcaagcct tctatttcgc
    20041 gtaaaacaat agccgttttc aaatcttccg gcaacgcatc aatggcggcg ttgacggttt
    20101 cgaccacctg gcgactggcc agcatggact ccggcgtact tatatcggtt aggttgtcga
    20161 tctggctaaa agtttcacca tcctcgttct cgatggtggt gggcgtgctg gggcggcgcc
    20221 cattggccac ttgccagttg cgcgcggtgt tgatggcgat gcgatacaac cacgtataga
    20281 aggcactttc accccgaaac tgcggcaagg cccggtacgc cttgacgaaa gcctcctgcg
    20341 ccacatcttc gatttccgag gggtcgcgga tcatgcgcga aagcaggcgc atgatcttgc
    20401 gctgatattt caggaccaac agatcgaagg cacgcttgtc gccccgctga acacgggcca
    20461 ccagctcggc atcgacctcg cgctcgctca ctggccaccc tgcgcggcaa cgattgccgg
    20521 ccgggcgatc cggctggcca ggcagacctg caaccgacgc caggcctgcg gcggaactcg
    20581 ggatttccag agggtgacct gcaacatagc atccttcgaa acaagacgat cctggtcgac
    20641 aaaacgcagg ctgatccaga acggcccgag ccagactcgc acgggacaca ggtgtgccat
    20701 gcgcgcgcca tcggacggcg cccattgcca gcggtcgtcc gcccccaggg aaagcacccc
    20761 cgacgacgcc tccgcgcgaa tccgccggga gcgttcacgt acccaccaca gcgacagacc
    20821 catagttagc acatatggcg aggccaggca atacgccgcg ccagccatgg ccgccgccag
    20881 cagcccgggc tcgagcagcg ccacccagcg cggacgcgag acaggccagg acagaacgac
    20941 gggcgcgccg acgtccggga tgcctggcct gactgatctg acggtgccgt gccgcatgcg
    21001 tggcgatcgg gcgctacgaa aggcgccgca cgatcatgga accgttggtg ccgccgaacc
    21061 cgaacgaatt cgacagcgcc acatcgatct tcatgtcacg cgcctgattc gcgcaataat
    21121 cgagatcgca ctcggggtcc tggctgaaga tattgatggt ggggggcgag acctgacgat
    21181 acacggccag cgtcgtgaat accgcttcga tgccgcccgc ggcccccaga agatggcccg
    21241 tcatggattt ggtggagttg acgacaagct tccgggcgtg gtcgccaaaa gccagtttga
    21301 gggcttcggt ttcgttgatg tcacccagcg gcgtggaggt gccatgggca ttgacgtaat
    21361 cgacctcgtc ggggttgacg ccaccgttgc gcagggcgtt gagcacgccg cgtcgcggcc
    21421 cgtcgcgatc aggcgccgtg atgtgatgcg cgtcggagct catgccatag cccgcgaact
    21481 cgccgtagat gcgcgcaccg cgcttgcggg cgtgttcgta ttcttcaagc atcagggcac
    21541 cggccccctc gcccagcacg aagccgtcgc gatcacgatc gaaaggacgc gaagccgtgg
    21601 ccggatcgtc gttgcgcatg gacagcgcac gcattgcggc aaacccacca atacccaatg
    21661 gcgacacggt ggactcggcg cccccggcca gcatgacatc ggcgtcaccg tactcgatga
    21721 gccgggccgc gtcaccaatg gaatgcaggc ccgtcgtgca ggccgacacc accgcgtagc
    21781 tgggcccttt gagacccagc aggatgctga gctgccctga aatgaggttg atgagtgctc
    21841 ccggcacgaa gaacggcgaa atgcgcttga gcccgcgctg cagggtttcg atctgggttt
    21901 cctcgatgcg cggcagaccg ccgatgccgg aacccatgat cacacccgcg cgctcgccgt
    21961 cggaatcgga gagatcaagg ccggaatcac gccaggcctg caaccccgcg gcgacgccat
    22021 aatggatgaa cgtatccatc tgccgggctt ctttcgcgga cagatactgc gtcacgtcga
    22081 aacccttgac ttcgccagcg atctgggaat tgagcgcgga tgcgtcgaag cgcgtgatgc
    22141 gtccgatgcc tgaccggccg ttgaccacgt tgtcccaggc gctttccacg tcgttgccga
    22201 tgggggaaac aatgcccagt ccggtaatga cgacacgtcg tttcacggaa gactcctcaa
    22261 atgaatgcac ggtctggtgc gggcccccgc cagattgtgg cgcgcacaga acgaagcgag
    22321 gcgccccact ctgttgcgca aagcccgccg accctggaac acgaaggggt ggatgccgag
    22381 ccgccgcgtc gatgcgccat tcccgcctga tcgatgagcg cgccgtgcgc tgatcggacc
    22441 aaggggttga ggcgatacgc cgcaaccggg caaaagccct gaatgtccaa acccggcggg
    22501 ctcgtgggag ccgctacggc gccggccggc tatgccagac gcgccacatg gattgataga
    22561 cttgagatta ctgcttgcta tgcgacgtga tgtagtcaac agcttgctga accgtggtga
    22621 ttttctcggc ctcttcgtcc gggatttcgg tttcgaactc gtcctcgaga gccattacga
    22681 gctcgaccat atcgagcgaa tcggcaccga ggtcgtcaag gaacgaagat tcgtttttga
    22741 tctcggcgtc attgacacca agttgctcag cgacgatctt cttgacgcgc tgttcgatgc
    22801 tttccatgca gatctccaag tgggaaaaat tcccgcgaat tatatagcca aatgaaaagt
    22861 ttcaaaggga caggctatga caaagacatg gcatcagcgg gcatttgttt cttgtgcgac
    22921 gaaagcgcgg attgcccggt gaaatacctc ccgaatggac agattttacc ctgttccatc
    22981 agtaccacac ataccctcta catatacaaa ccgccgttca cgtgcaccgt tgtgcccgtg
    23041 atgtaatcag cctgcggcga cgccaggaag gcggcggcgt tggcgatgtc cgtcggcaag
    23101 cccaggcgcc ccagaggcac ttgcgccaga atggattcgc gctgcgcatc gctcaatgcc
    23161 cgggtcatgt cggtgtcgat gaagccaggc gccacacaat tgaccgtgac attgcgactg
    23221 cccagttcgc gcgccaggga gcgcgtcatg ctggtgacgc ccgccttggc cgcggcatag
    23281 ttggcttgcc ccggattgcc gctgaatccg atcaccgagg tgatattgat gatgcgcccc
    23341 cagcgggcct tcatcatgct gcgcatgacg gcgcgcgaaa ggcgaaacac cgccgtgagg
    23401 ttggtattga tgacggcgtc ccaatcatca tccttcatgc gcatggccag gttgtcccgc
    23461 gtgatgccag cgttgttgac caggatgtgt gggccgccgt cttccttgcc aagctccgcc
    23521 accagggcgt cacaagcggc cgcgtcggtc acgtccagca ccacgccccg gccgcccgcc
    23581 ccggccagcg tggcgctgat ggcatcggca ccggtccggg aggtggcggt gccgatgacg
    23641 cgcgcacccc gcagggcgag ttcgcgaacg atggcctgac cgattccccg ggtggcgccc
    23701 gtcaccaggg cggtcttgcc ttcgagatgg cgttcttgca tggtgctact ccttgatgac
    23761 ggccagcgtg gcctcgagcg aggcggggtc gctgacgttg aaactttgca gatccttgtc
    23821 gatccggcgt gtcaggcccg acaggacctt gccgggacca cattcgatca cgtgcgtgat
    23881 gccccgatcc ttcatggcgc ggatagtctc gacccagcgc acggggtgcc aggcctgccg
    23941 caccagtgcg tcccggatgg cggccggatc ggacgggctc gcaacgtcga cgttgttgat
    24001 gagttcgata cccggtgccg ccatcgacac gccggccaat gccttgccca atacagtggc
    24061 ggcgggttcc aacagccggg aatgaaacgg tgccgagacc ggcagcggca acgcccgctt
    24121 ggcgcccgct gccttggcca gttcgcaggc gcgtgcgacg gcggtggcat ggcccgcgat
    24181 caccacctgg gcgggggcat tgaaattcac agcctcgacc atttcccccc gggcggcctg
    24241 cgcgcagaca tcccgcacga catcgtcgtc gagcccgaga atggcggcca tcccacccgt
    24301 gccgaccggc acggcggtct gcatggcgtc ggcacgaacc cgcacgatgc gcaccgcgtc
    24361 ttccaacgcc agcgaacgcg cggcggtcag ggcggaatac tcgcccagac tatgcccggc
    24421 aaccagggaa ggctcggggc cgcccgctgc taaccaggcc cgatacatgg cgaccgccga
    24481 agccagcatc gccggttgtg tgttggtggt cagattcaat tgctcggccg gaccaccggc
    24541 gatgaggcta gccagatcct ggccaagcgc ggccgacgcc aaacccaggg cttccgccac
    24601 aaccgtgttg ccggcccacg cgtcgagcat gcccaccgac tgggagccct gccccggaaa
    24661 aacgaatgcg attttcatga tgaacccaag gaaaaaatta gtcgcagcga gaacaaagac
    24721 aaaatcgtcg agcgcggccg cccgggctgt gcgaatcgaa tcgggtcaaa tccgcaccag
    24781 cagggagccc caggtgaaac cgccgcccac cccctgcatc aatacctgtt ggccggcgcg
    24841 gatgcgacca tccctgaacg cgacatcgaa cgcgagcgga acgctggcgg ccgacgtatt
    24901 agcgtgccga tcgagcgtga tcacgatttt ctcagtgggg atccccagtt tgcggcccag
    24961 catgttgaga atgcgcacat tggcctggtg cggaatcatc cagtccacat cggagagcgc
    25021 caccccggct tgctcgcaga cgtcccgcgc cgattgcgca agcaccgtga ccgccagctt
    25081 gaacaccgcc tggccatcca tccgcaggaa aggatcgccc tccaccttgc cgcgcgaaat
    25141 gccgcccgcg gtacagagaa tgcccgcttg gcggccatcg gcgccgagtt tcgcgcccga
    25201 aatcccgggt tcgtcggagg cccgcaacac cacggcgccc gcaccgtcac cgaacaggac
    25261 acatgtgccg cgatcattcc agtccagaat gcccgaaaat acctcggcgc cgataaccag
    25321 tgccgtgcgg gcccggcccg agcggatgaa tgagtccgcg gtgatcatcg catagacgaa
    25381 accgctgcaa accgcctgga catcgaacgc ggcgccaccg ctcgcaccca gtccggcctg
    25441 cacgagacag gcggtgctcg ggaaaatgaa atccggtgtc gaggtggcga cgatgatgag
    25501 atcgaggtcg gtggcggtga cgcccgcgtg ctcgagcgcc gcacgcccgg cgttgatggc
    25561 caacgagctg gtcttgacac ccggctcggc gatgtgccgc tgacgtatgc cggtgcgctc
    25621 ggtgatccag gtatccgagg tttcgatctg ccgcgtcgcc agatcggcgg ccaactcatc
    25681 gttggaaacg actcgggggg gcaaataact ccccgaccct attatcttcg cataaggcat
    25741 gacaggcttc cgtcaggcat cttgtcagga cacagggttc gacaagtcgt tggtgggctt
    25801 cgccgatgag gccgccgcat cctgcaggcg ctggcgaaga tgatccatgg cattcatggt
    25861 gccatcgagc aggccattga gcacagcctc gtgcgcgcgt tgcagcgcgt agccgaaagc
    25921 gtaaacgtcg gcagacccgt ggctttttat gacgataccc cgcaaccccc gcagggcggc
    25981 gccattgtag cgccggttgt ccacccggtc gcgcaggcga ttgagaacag gtttggacag
    26041 catgccggcc gtcatcgaca gggcgtcacg ggtgaattcc tgacgcagca tgctggcgac
    26101 cattttcgcc acaccttcga tggacttgag cacgacattg ccgacgaatc cgtcacagac
    26161 cacgacgtcg accgtgccct tgcagatgtc gtcaccttcg acattgccgt aaaaattcaa
    26221 tccactggcg cgcaggagat cagccgcctg tttgaccact tcgttgccct tgatgacttc
    26281 ctcaccgatg ttgagcaagc ccacggtcgg gttgtcatgg aggtcaaccg tcttgaccag
    26341 ggccgtgccc atgatggcga actgcagcag gtggtcggca gtgcaatcaa cattggcgcc
    26401 caggtcgagc atggtggtgg cgccaccctt ctggttcgga atggacgtgg caatggcagg
    26461 acggtcgatg ccatcgagcg tcttgagcac ataccgcgat atggccatcc aggcgccggt
    26521 gttgcccgcc gagatacagg cgtcggcagc accgtctttg accgcctgcg ccgccacccg
    26581 catggaggag tccttcttgc ggcgcaacgc gacctcgacg gagtcttcca tgctgaccac
    26641 ttccgaggcc ggcaatattt cataccagtc gtcctgacgc accccgatct tgcgaatgcc
    26701 agcctggata gcctgggcat ctcccaccag caggaaccgg gtatcagggt gttcttgcgc
    26761 gaacctgtgg gcagcgggaa tggtcaccgg caagccgatg tcgcccccca tgcagtcgac
    26821 agctatgcgg attcgggtct tggacatcga cgaaggcgcg aaccttgaac cgatccggtc
    26881 agtcgtcgtt cttggtctgg atcactttgc gcccacggta cacaccgttg gggctgatgt
    26941 ggtggcgcag gtggagttcg ccagtcgttg gctcgacagc cgtggccggc gcgccgagga
    27001 aatcgtgaga gcggtgcatg ccgcgcttgg atgggctctt tttgttttgc tgaacagcca
    27061 tgatgactcc tggtgacggg acgccattgt acgcgatcat cccgtgaagt tgaatttaca
    27121 acgtcggttt tttgagtttg cccaacaccg aaaacggcga tgggcgcttt tcgtcagact
    27181 ccgaatcagc cggcgaggcg gattcggacg gacatgcatc gtgccggggc acatagggca
    27241 gaccgaggat gatctcatcc tcgatcaact ccagaatatc gagccggtgc gatgcggcga
    27301 tacgctcgac cgcgacacca tttccgtccg gatcgtgcga gccgccgtca tccagatcgg
    27361 cttcggccac cacctgaagc cgggtcgaga catcgagcgg ctgggcgaaa gcctcgaggc
    27421 agcgctggca ctcgagcaag acggccgttt tcacggacac ccacaggaag acttcccccc
    27481 ccgtgttctt gtcggcccgg accgaccact ccaccgactg atcggcctgc tcgggcaacc
    27541 cggcgcataa acggccgaag tcgcgcaacg acaccacgcc cgccacggcc gaacccgacc
    27601 gcgccagttc atacgtatcg atataccgca ccgcgctcac gcagacacct gctccaatcc
    27661 tccaccgccc caatacagct tcgcagacca aaggccccga gccgcaacag ccctaagcca
    27721 cgcagccttg tatccagcct tgtatcaata cagccttgta tttaaacgga aaaggaaaca
    27781 ttcgatagta acctgactat accgggcggg tcaaacacaa cggtagcgag tgcgtcccca
    27841 ccgcaacgct catccaacgc ttgtcgcgcg gcgacgcccg agccccatca gggcatacag
    27901 gatgatggaa ccgaatgtgg cggtgccgat cccgtccagc ttgaaatcgc caaactgtag
    27961 ggaaaaattg cccgccccga gtatcagtgt caccgccgtg acaatgaggt tgcggttatc
    28021 ggcgaaatcc acctgattga cgacccagat ccgcgcgccc gccacggcga tgagcccgaa
    28081 caccacgatg gacatcccgc ccagcaccgg cccgggtatc gtatgaatca gcgcgccgaa
    28141 cttcggggaa aaacccagga cgatggcgat caagccagct atcacgaaaa ccagcgtcga
    28201 gtagatgcgg gtgaccgtca tcacgcctat gttctccgcg taggtggtca cacccgtccc
    28261 accaacggac cccgacacca tggtggccac ggcgtccccc aggaaagcgc ggcccaggta
    28321 aatatccagg ttgcgccccg tcatggcgct gaccgccttg atgtggccca gattctcggc
    28381 caccaggatc accgccaccg gcgcgataag gccgatggcg tgggtgctga acacaggtgc
    28441 ctcaaaatgc ggcagcccca gccagggcgc cgcggccacc gcgctaaagt ccatgggacg
    28501 ccccaagccc agtccgttgg cgcacaccgc atacaacacg caagccagca gtagtccgac
    28561 caggatcagc aagcgttgcg tcgtcccacg cgtatagaca gcgataccgc caacacacaa
    28621 taccgtcacc agcgccatga tcgcttcaaa gttcgtgccg cccatggctc ccttggcggc
    28681 gatgggcgcg aggttcaggc cgatcaccgc gacgatggct cccgtgaccg ccggcggcat
    28741 caaggcgtcg atccagcccg ccgcatcccc ggcgcgcgcg ttcacccacc acacgatgat
    28801 tccgatcacc gcatacacca ccccgcagac gatgatggcg cccagcgcca gaggaatatt
    28861 gacgttgtgc cccgcacccg catagccggt gacggtgatc accccgccga tgaaggcgaa
    28921 actggaaccc agatagctgg gtacccgccc tcccacgaac agaaagaaaa tgagagtgcc
    28981 gatgcccgac atcagaatgg cgacattggg gtcgaaaccc atgagcaacg gcgccaggat
    29041 ggtcgaaccg aacatggcca ccacgtgttg cgcgcccatc gcgacgttct gcggccagga
    29101 caggcattca tccggctgta ccaccagtcc ggcgcctgtg ctttgaatcc ggcgccaacc
    29161 gggaaaatat gattgcgaca tggtgtcgtt tcctgttttt gttgttgatt cgattcggct
    29221 tctggatgaa gggcgcgcca cctcacacgc tccgtcgagg cgaaagtgta cggtcccgcg
    29281 ccccgggccc gcaagaacaa ccggccggaa gcctgggcga ataggcgata atgaagctca
    29341 ttcagacaac acgcgccaac ctgacgcgac caaaccctga tcccatgcgt ctcatcctgg
    29401 cttccagctc ccgctatcga cgggacatgc tggcccgact gggcctcccc ttcgacacag
    29461 aagccccggg ggtcgacgaa acgccacaga acggcgaaga acccgaatcg ctggcacgcc
    29521 gattgtccct ggcgaaggcg ttggcggtgg cgtcgcgcca cccaggcgcc gtgatcatcg
    29581 gctcggacca ggtcgcgtcc ctggagggag ctcccatcgg caaacccggc gggttcgatc
    29641 gcgcgcgcga acaattgcgc cgactcgccg gccgaaccgt cacgttccac agcggcctgt
    29701 gcgtcaccga tggcctacat cacgaagtgg tgtgtgtcga cacgttttgc cggttccgcc
    29761 ccttgaaaga ccaggacatc gatacgtatc tgcggcgcga acagcccttc gacaccgccg
    29821 gtagcgcgaa agccgaaggc ttgggtgtcg cgctgatgga acgcatgacc tcggacgacc
    29881 ccacggccat catcggcctg cctctcatcg aactctgccg cctg
//
LOCUS       DASTNK010000117         8085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_832443, whole genome shotgun sequence.
ACCESSION   DASTNK010000117 DASTNK010000000
VERSION     DASTNK010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8085
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832443"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFR20_08625"
     CDS             <1..216
                     /locus_tag="VFR20_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230624.1"
                     /translation="AADFPDFRFGTVCTPCTHNPIGSKGCGEAGTIGSPPAVINALLD
                     ALEPLGVADIDMPATPYRVWQAIHAAR"
     gene            235..1026
                     /locus_tag="VFR20_08630"
     CDS             235..1026
                     /locus_tag="VFR20_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEU0230625.1"
                     /translation="MYAFTYERPTTQADALRLVQAGGQPLAGGQTLLGSMKLRLAAPE
                     QLVDLGAIPGLAGIRQESGAVVIGAMTRHADVAASADVAAAIPALAGLADGIGDRQVR
                     AMGTIGGSVANNDPAADYPAALLALGATVHTTSRQIKADDFFQGLFATVLEPGELITA
                     IGFPVPRRAAYVKFPQPASLFALIGVFVAQFDHEVRVAVTGGGNGVFRHQALEAALKD
                     RFSPDAVVGVPTDESEMSSDLHGSGAYRAHLVGVMTRRAVVRALA"
     gene            1027..1968
                     /locus_tag="VFR20_08635"
     CDS             1027..1968
                     /locus_tag="VFR20_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU0230626.1"
                     /translation="MVSAGASAAPTGLPGLGSIDALVSALETRAYFADRRLATAVFLA
                     LRLGRPLLLEGEPGVGKTELAKALASVLGRELLRLQCFDGLEQREALYEWNYAAQLLH
                     LRAVEGRAPVADLEQEVYQPRFLVRRPLLQALQAPAPGAVLLVDEVDRADEPFEAFLL
                     EYLGEYQVSIPELGTVRATVAPVTILTSNRTRELNDAVKRRCLYHWLDYPDRARELAI
                     VRALVPQAPEALANQVAAFVQQLRTAPYDAHFQRAPGIAETVEWARALVALDTLVLDP
                     EIVVDTAGILFKQRDDVAALDPALVSEALRSADQAAA"
     gene            1978..3219
                     /locus_tag="VFR20_08640"
     CDS             1978..3219
                     /locus_tag="VFR20_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0230627.1"
                     /translation="MTSTRDPVSAPRPHWVSQLGDARRGKLSGNLSAFGRALRRAGVP
                     VDASRIALAQEALQCVGVAHKQDVSAALETVLVSREQDRVVFREMFEAFFRDPELAHQ
                     LLARMVPREAREAGPPRTSPRVRAALAPPAAGQPRAESDVEFDAAMTASDLARLKEAD
                     FNRLTASEYDLVRRLVRDVALPLPTVDGRRTRAGWRGPRVHWGRTLRAAARSGGEVMI
                     LHRLRRRPQSLPLLMLVDVSGSMERYTRTLLAFLHASTAHVQVSGQTLSVRREVFAFG
                     TELTDLGAAFRLADTDAMLLAAGDLIHDFAGGTRLGDCLGGLRRQYSRCLVGRRTIVL
                     IVSDGLDTGEPEDLARELAWLKRRSRRVLWLNPLLRFDGYEPSARGAAVLHRMADAML
                     AVHNVSRLQELAGAIAALMKQ"
     gene            3237..3836
                     /locus_tag="VFR20_08645"
     CDS             3237..3836
                     /locus_tag="VFR20_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEU0230628.1"
                     /translation="MDMQSSRQLGVTQRQAWEALNDPQVLKVCVPGCESIEPAGDNAY
                     ALVSTIKVGPVAAKFKSTIQLTDIQAPDHYTLNFDGNGGAAGFGKGSAKVTLTPHEEG
                     CELAYTVNATVGGKIAQVGQRLIDGVAKSMAQSFFKRFDEEMQRRYPEQSATPATADS
                     GLPVSPTGATAARRIPIWIWVVVALLIVLGIAVESRITL"
     gene            3864..4904
                     /locus_tag="VFR20_08650"
     CDS             3864..4904
                     /locus_tag="VFR20_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEU0230629.1"
                     /translation="MENLNITVLRALRDWRQEGRHAVLATVVHTWGSSPRPIGSIMAM
                     REDGVIVGSVSGGCIEDDLIARYTQPDARDTLPRNGPPRFIKYGVTADEAHQFGLPCG
                     GTLEVVLEFDPQTQALVDLVQALDAGKLMHRRVRLADGQVRLEVSDVPADLGMDEQEL
                     VNTFGPEYRMLIIGAGQMSEYLATMAQFCGFAVTVCDPRKEYNSIWNVPGVRVVATMP
                     DDTVAEFRPDARSCVIALTHDPKLDDLALLQALETPAFYIGAIGSRRNNHTRHQRMIE
                     YLDQTEESLARLRGPVGIYIGSKTPPEIAVSVMAEVLAVKNGVPLSRDNDVEHVKDQL
                     GLVSAGSVCSLG"
     gene            5083..5385
                     /locus_tag="VFR20_08655"
     CDS             5083..5385
                     /locus_tag="VFR20_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011548450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEU0230630.1"
                     /translation="MNLDLALKALDNAHRRAVLSAMRDPRKHFQPLGGTDPLTDGVCV
                     TQVSDLLGVNQSTASSYMALLRDAGLVSATRVGKYTRFKRNEQTLAAVAAAITNQL"
     gene            5467..6654
                     /locus_tag="VFR20_08660"
     CDS             5467..6654
                     /locus_tag="VFR20_08660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0230631.1"
                     /translation="MKIPLRYQILLLALTTFVAGANEYILAGELDLVARGLGISVELA
                     GQLITVYALIYGFCVPVVVALTGHFGRRAVLVTAMIFYSIFSGLCFFVDDFWVFTAMR
                     LLQALSGGLAVVSSLSTAATLAGPERRGRAIATVIMGFTMSLIIAVPVGREIAIRFGW
                     NAVFPVIALLALITALVQRSVLPVLSPAASIPLRDQVAMLARPTIQGGLLVTVLWMAG
                     YCLTYSYLTPYLLTVQHIPGNWMSPLLLVFGIASLIGARMGGSFNDRHGHHFTLVTFK
                     LLQSLFLVALPVAGLVWSHASLSVIAVVLILWSITAWACGPSQQVRAASINANASGVL
                     VGLNQSAMQIGIATGSALGGVAAGALGLSWLPWLSAVAVLVALALMVRLRRKVSVAHI
                     VTS"
     gene            7181..8074
                     /locus_tag="VFR20_08665"
     CDS             7181..8074
                     /locus_tag="VFR20_08665"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0230632.1"
                     /translation="MYKAYVLLALTTLFWAGNSIAGKLAVGHVSPMILVTARWASVMA
                     VLYVFGKRRIATDWPTIRPRLFHLLLLGALGFTVFSVALYYALVYTTAINASILQGGM
                     PLFVFAASFVLFSSRIGVEQAVGFVLSFVGVVVIAMRGEWARLIGLDINFGDALMLVA
                     IIAYGVYTAMLRSKPRVHWASLMFILCLGATLASLPMLVFEAARGQTIFPDMRGWGVI
                     LYIVIFPSLVGQVLYIQAVELIGANRAGLFINLLPVWGAFLAVAILGEQFHVYHAVAL
                     VMILVGIGLAEYGGRRLSRQG"
BASE COUNT         1325 a         2413 c         2665 g         1682 t
ORIGIN      
        1 gcggcggact tcccggattt ccggttcggc acggtctgca caccctgcac gcacaacccg
       61 atcggcagca agggctgcgg cgaggccggc accatcggct ctccaccggc ggtgatcaat
      121 gccctgctgg atgcgctcga accgctgggg gtggccgaca tcgacatgcc cgcgacgcct
      181 tatcgggtgt ggcaggccat tcacgccgcc cggtgaccca cacaggagac acacatgtac
      241 gcattcacct atgagcggcc caccacgcag gccgatgccc tcaggctggt ccaggcgggc
      301 gggcagccgc tggcgggcgg gcagactttg ctggggtcga tgaaactacg tctagccgct
      361 ccggaacagt tggttgatct aggcgcgatt ccaggcttgg cggggatccg ccaggaatcc
      421 ggcgcggtcg tgatcggtgc catgacgcgg cacgcggatg tcgccgcaag cgcggacgtc
      481 gcggccgcca ttcccgcctt ggcggggttg gccgatggca taggcgaccg gcaggtgcgc
      541 gccatgggca cgataggcgg gtcggtggcc aacaacgatc cggcggctga ctaccccgcg
      601 gcgctgctgg cgctgggcgc caccgttcat accacgagtc gtcagatcaa ggccgacgac
      661 tttttccagg ggctctttgc gacggtgctg gagcctggtg aactcatcac cgccatcggt
      721 ttcccggtgc ccagacgcgc cgcctatgtg aagttcccgc agcccgcctc gttgttcgcc
      781 ctgattgggg tgttcgtggc gcagttcgat cacgaggttc gggtggcggt gacgggcggc
      841 ggcaatggcg tgttccgtca ccaggccctg gaagccgcgt tgaaggatcg cttctcgccc
      901 gatgccgtgg ttggcgttcc gaccgatgag tccgagatgt cgtccgacct gcacgggagt
      961 ggcgcttatc gagcccatct ggtcggggtc atgacgcggc gcgccgtggt tcgggcgttg
     1021 gcataaatgg tctctgcggg cgcgtcggcg gcaccgactg gtttacctgg cctcggttcc
     1081 atcgacgcgt tggtgtcagc cctggaaacc agggcctact ttgcggatcg acgcctggcc
     1141 accgccgtgt tcctggcgct caggctgggg cggcccctgt tgctggaagg cgagcccggc
     1201 gtgggcaaga ccgagctcgc caaggcgttg gccagtgtgc tgggacgtga actgctgcgg
     1261 cttcagtgct tcgatggcct ggagcagcgc gaggcgttgt acgagtggaa ctatgcggcc
     1321 cagctgctgc atctgcgggc ggtcgagggc agggcgcccg ttgccgacct tgagcaggaa
     1381 gtctatcaac cccggttcct ggtgcgccgc cccttgctgc aggccctgca ggcccccgcg
     1441 ccgggcgccg tgctgctggt cgatgaagtc gatcgcgccg atgagccgtt cgaagccttt
     1501 ttgctcgagt acctgggtga gtatcaggtc agcatccccg agctcggaac ggtgcgcgcc
     1561 accgtcgcgc cggtgacgat actgacaagc aaccgcacgc gcgagctcaa cgacgcagtc
     1621 aagcgccgct gcctgtacca ctggctggat taccccgatc gcgcacggga attggccatt
     1681 gtgcgggcgc tggtgccgca ggcccccgaa gccctggcca atcaagtggc ggcctttgtg
     1741 cagcaactgc gcaccgcgcc gtatgatgcg cattttcagc gtgcgcccgg catcgccgag
     1801 accgtggaat gggcgcgcgc cctggtcgct ctggataccc tggtgcttga cccggagatc
     1861 gtcgtcgaca ccgccggcat cctgttcaag cagcgcgacg atgtggccgc gctggacccc
     1921 gcgctggtgt ccgaggcctt gcgatcggcg gaccaggccg ccgcatgacg ggccgccatg
     1981 acatcgaccc gcgatccggt ctccgcgcca cggccgcatt gggtgtccca actgggcgac
     2041 gcgcgtcgcg gcaagctcag cggtaatctc agtgcctttg gccgcgccct gcgtcgggcg
     2101 ggggtgccgg tggatgcctc tcgaatcgcc ctggcccagg aagccctgca gtgcgtgggc
     2161 gtggcacata aacaggatgt cagtgccgcg ctggagacgg tgctggtcag ccgcgagcag
     2221 gaccgcgtcg tgttccgcga aatgtttgag gcatttttcc gggatccgga actggcccat
     2281 caattgctgg cccgcatggt gccgcgcgag gcgcgcgaag ccgggccgcc gcgtacaagc
     2341 ccgcgtgtgc gcgcggcctt ggccccaccg gccgccggcc aacctcgtgc ggaatcggat
     2401 gtcgagtttg atgccgccat gacggccagt gatctggcgc gcctgaaaga ggcggacttc
     2461 aatcggctca cggcgagcga atacgacttg gtgcggcgcc tggtgcggga tgtggcgttg
     2521 ccgctgccca cggtcgacgg ccgccgcacg cgcgccgggt ggcgcggccc gcgcgtgcat
     2581 tgggggcgca ctttgcgggc ggcggcgcga agcggcggcg aagtgatgat tctgcatcgg
     2641 ctgcggcggc ggccacagtc cttgccgttg ctgatgctgg tggatgtttc gggatccatg
     2701 gagcgctaca cgcgcacctt gttggccttc ctgcatgcgt cgactgcaca tgttcaggtc
     2761 agtgggcaaa ccctgtcggt gcgccgcgag gtctttgcct ttggaaccga gctgaccgat
     2821 ctgggggcgg cttttcggtt ggcggacacg gatgccatgc tgctcgccgc gggcgacctc
     2881 attcacgatt tcgcgggggg gacgcgcctg ggtgactgtc tgggcggttt gcggcgacag
     2941 tactcccgct gcctggtggg acggcgcacg attgtgctga tcgtcagcga cggcctggat
     3001 accggcgagc cagaggatct tgccagggag ctggcctggc tcaagcgccg ctcgcgtcgc
     3061 gtcctgtggc tcaacccatt gctgcgcttt gacggatatg agcccagcgc gcgcggcgcc
     3121 gccgtgctgc atcgcatggc cgatgctatg ctggcggtac acaatgtcag tcgtctgcag
     3181 gagctggcgg gtgcgatcgc cgcgctcatg aaacagtgat tcaagaggag agaacgatgg
     3241 acatgcagag ttcgcgtcaa ctcggtgtca ctcagcggca ggcctgggag gcgctcaatg
     3301 atccccaggt cctgaaggtg tgcgtgccgg gttgcgagag catcgaaccc gccggcgata
     3361 acgcctacgc cctggtcagc accatcaagg tcggccctgt cgcggccaag ttcaagagta
     3421 cgatacagct gacggatatc caggccccgg accactacac gctcaacttt gatggaaacg
     3481 gcggggccgc cggcttcggc aaggggtcgg ccaaggtcac cctgacccct cacgaagaag
     3541 gctgtgaact ggcctatacg gtcaacgcaa ccgtcggcgg caagatcgcc caggtgggcc
     3601 agcgcctgat cgacggggtg gccaagtcga tggctcaaag ttttttcaag cgcttcgacg
     3661 aagaaatgca gcgccgctac ccggaacaga gtgccacgcc cgcaactgcc gattccggcc
     3721 ttcccgtctc gccgacgggg gccacagccg cgcgccgcat tcccatctgg atctgggtgg
     3781 tggtggcgct gttgatcgtg ctgggtattg ccgtggaatc aagaattacc ttatagagag
     3841 aggagacttc aaggagtcga atcatggaaa atctgaatat tactgtcttg cgcgcgctgc
     3901 gcgactggcg gcaggaaggt cgccatgctg ttttggcgac tgtggtgcat acctggggct
     3961 cgtcgccccg gcccatcggt tcgatcatgg caatgcgcga agacggcgtg atcgtggggt
     4021 cggtatcggg gggctgcatt gaagacgacc tgatcgcccg ctatacccag cccgatgccc
     4081 gcgacacttt gccccgcaac gggccgccac ggttcatcaa atacggcgtc acggcggacg
     4141 aggcccacca atttggcctg ccctgcggtg gcacgcttga agttgttctc gagttcgatc
     4201 cgcagaccca ggcgctggtg gatctggtgc aggcgctgga tgccggaaaa ctcatgcacc
     4261 gacgcgttcg cctggccgat ggccaggtgc gcctggaagt ctctgatgtt cccgccgatt
     4321 tgggcatgga tgaacaggag ctggtgaata cgttcggccc ggaataccgg atgctcatca
     4381 tcggtgccgg acagatgagc gaatacctgg ccaccatggc gcagttctgc gggttcgcgg
     4441 tcaccgtgtg cgatccgcgc aaggagtaca acagcatctg gaacgtgcct ggtgtgcgcg
     4501 tggtggccac gatgccggac gacacagtgg cggaattccg gcccgatgcc cgcagttgtg
     4561 tgattgcctt gacgcacgat ccaaaactgg atgacctggc gcttctgcag gcgctggaaa
     4621 caccggcgtt ctatatcggc gccatcggca gccggcgcaa taaccatacg cggcaccagc
     4681 gcatgatcga gtacctggac cagaccgagg agtccctggc ccgtttgcgt ggccccgtgg
     4741 gtatctatat tggcagcaag acgccacccg agatcgcggt gagcgtcatg gccgaggtcc
     4801 tggcggtgaa aaacggtgtc ccgctatcgc gtgacaacga tgtggagcac gtcaaggatc
     4861 aattgggctt ggtttcggcg ggttctgtgt gctcgcttgg ttgatggttg tgggcgtgac
     4921 ggtgccgaac atgtggcaat gtggatttgt ggcaatttac ggagctgtca atcatgagcc
     4981 gcctaaccat cgacatgacc gatcaacaac atcagaacct gaaggtattg gccgcactgc
     5041 atacatattg acaatttccg atatgtcgaa tagtatggcg tcatgaatct ggatcttgcc
     5101 ctcaaagccc tcgacaacgc gcaccggcgc gccgtattgt cggccatgcg cgacccgcgc
     5161 aagcacttcc agccgcttgg cggcaccgac ccgcttaccg atggcgtctg cgtgacccag
     5221 gtgtcagacc tgttgggggt caaccagtca actgcgtcga gctacatggc gttattgcgc
     5281 gatgccggct tggtgtcggc gacacgggtc ggtaaataca cgcgcttcaa gcgcaacgag
     5341 caaaccctgg ccgccgtggc ggccgccatc acgaatcaat tgtagagccg ctctccaata
     5401 gtgctattgg agtctcgctt tcgattttcg aatcgataat ttgcgaatca tagatttaaa
     5461 agcatcatga aaattcccct tcgatatcaa atactgctgc ttgccctaac cacttttgtt
     5521 gcgggggcga acgagtacat cctggctggt gaattggatc tcgtcgcccg tggactgggc
     5581 atctcggtgg aactggccgg acagctcatc accgtctacg cactgatata cgggttctgc
     5641 gtgcccgtgg tggtggccct gaccgggcac ttcgggcgca gagcggtgct cgttacggcg
     5701 atgatcttct attcgatttt cagcggcctg tgtttcttcg tggacgactt ctgggtcttc
     5761 accgcaatgc ggttgcttca ggcgctgtcg ggcggcctcg cggtcgtgtc gagcctgtcg
     5821 accgcggcaa ccctggccgg tccggaacgc cggggccgtg ccattgccac cgtcatcatg
     5881 ggcttcacga tgtccctcat cattgccgtt ccggtggggc gcgagatcgc gatacgcttt
     5941 ggctggaacg cggtttttcc ggtcatagcg ctgctggcct tgataacggc ccttgtccaa
     6001 cgtagcgtat tgccggtgtt gagccccgcg gcatccattc cgcttcggga tcaagtggct
     6061 atgctggccc gccccaccat ccagggaggt ctgcttgtga ccgtcctgtg gatggcggga
     6121 tactgcctca cctatagcta tctcacgccc tatctgctca ccgtgcagca cattccaggc
     6181 aactggatga gcccgctgct gctggtgttc ggaatagcga gcctgatcgg agccaggatg
     6241 ggcggaagtt tcaatgatcg acacggtcac catttcacac tggtcacgtt caagcttctc
     6301 cagtcgcttt ttctcgtcgc cctgcccgtg gcgggtctcg tgtggtcgca tgcatccctt
     6361 tctgtgatcg ccgtggtatt gatactgtgg tcgatcacag catgggcctg tgggccatca
     6421 caacaggttc gcgcggcgtc catcaacgcg aacgcgagcg gtgtcctggt gggtttgaat
     6481 caatccgcca tgcagatagg catcgccacg gggtccgcgc tcggcggtgt tgcggcgggc
     6541 gcgcttggtt tgtcgtggct tccgtggtta tcggccgtgg cggtattggt cgccctggcc
     6601 ctgatggtga ggttgcggcg gaaagtaagc gtggcgcaca tcgtgacatc gtgatgctcg
     6661 ttatcgcttg atgagtgggt gtagccagcc tcttcggccg cgaccttgag gcctttcccc
     6721 agttcgcctg ccaggcgctc cagcaatcag tcggcgcttg gccgctcgag cctggccacc
     6781 agggccttgg cgatccgtgg gttcgccaga ccgttgaaca gaccattgcc atatgggctg
     6841 gggtgtgcga caagatggtg gagcaacccg atgatcagaa tgccgccggc tatagtgttt
     6901 tgagttgcaa atagttcgtc acaatgctcg catgtcgtca agcatgtgca tgtcgaccat
     6961 caaatttccg gggttctaat gaccattacc gctttgacgc caaggcaacc tgttccgatc
     7021 caccgttccg ggacccgttg ggcgctatcg actttgccat tgcaaacgac tatcctgccc
     7081 gcagcgagca tacgggcggt ttgtaggccc gccgtgatgt atcgctgaaa aaaatataag
     7141 gcccgggaga ttcgcccggc tcctcctcgg agatcatgcc atctataaag cctacgttct
     7201 tctcgcgttg acaaccctct tctgggcggg aaactccatc gccggcaagc tggcggtcgg
     7261 ccatgtgtcg ccgatgattc ttgtcaccgc ccgctgggcg agtgtgatgg cggtgctgta
     7321 tgtgttcggg aagcggcgga ttgccaccga ctggccgaca atccggccac ggctgttcca
     7381 tttgctgctg ttgggggcgc ttggcttcac cgtcttcagc gtggcgctgt attacgcgct
     7441 ggtctatacc accgcgatca atgccagcat tttgcagggc ggcatgccgc ttttcgtatt
     7501 cgccgcgagc ttcgttctct tttccagccg cataggcgtg gagcaggcgg tcggcttcgt
     7561 gctgtctttc gtgggcgtgg tcgtcatcgc tatgcgtggc gagtgggcgc ggttgattgg
     7621 ccttgacatc aatttcggcg atgcgctgat gctcgtcgcc atcattgcgt atggtgtcta
     7681 cacagcgatg ctgcgcagca agccccgggt gcattgggca agcctgatgt tcatattgtg
     7741 cctgggggcc acgttggcgt cgcttcccat gctggtgttt gaagcggcgc gaggccagac
     7801 catttttccg gatatgcggg gttggggggt gatcctctat atcgttatct tcccatccct
     7861 cgtgggccaa gtcctttaca ttcaggcggt ggagctgatc ggcgcgaatc gggccggcct
     7921 gttcatcaac ctgctgcctg tgtggggcgc gtttctggcg gttgccatac ttggggagca
     7981 attccacgtt tatcatgcag ttgctttggt gatgattctg gtggggattg ggcttgccga
     8041 gtatgggggg cggaggttga gtcggcaggg gtgatggggg agagg
//
LOCUS       DASTNK010000118        14544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_461549, whole genome shotgun sequence.
ACCESSION   DASTNK010000118 DASTNK010000000
VERSION     DASTNK010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14544
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461549"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2209
                     /gene="gyrA"
                     /locus_tag="VFR20_08670"
     CDS             <1..2209
                     /gene="gyrA"
                     /locus_tag="VFR20_08670"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU0230633.1"
                     /translation="SRLPNLLVNGSSGIAVGMATNIPPHNLSEVVDGCLYCLRNPDCT
                     VDELIELIPAPDFPTGGIIYGMSGVREGYRTGRGRVIMRAKTHFEDMEKGHRQAIIVD
                     EIPYQVNKKSLQERIAELVNEKKVEGISDIRDESDKDGMRLVIELKRGEVPEVVLNNL
                     FKNTQLQDTFGMNMVALVDGQPRLLNLKQMVEYFLFHRREVVTRRTVFQLRKARERGH
                     VLEGLAVALANIDDFIAIIKAAPTPPVARQELMARSWDSSLVREMLARADEGAELGGH
                     AAYRPEDLPDVFGIQPDGLYRLSETQAQEILNMRLQRLTGLEQDKIVAEYKDVMAAIA
                     DLLDILARPERITTIISDELAAIKAEFSTNVKDIRRSAIEHNATELDTEDLITPMDMV
                     VTLSQTGYIKSQPLSEYRAQKRGGRGKQATAMKENDWVDQLFIANTHDYLLCFSDRGR
                     VYWLKVWEVPQGTRNSRGRPIVNMFPLTDNEKITVVLAIKQFSDDHYIFMATSRGTVK
                     KTPLSDFSNPRKAGIIAVDLDDGDYLIGADLTDGEHDVMLFSDSGKAVRFDENDVRPM
                     GRTARGVRGMMLEEGQSVISLLVARDESQSVLTATENGFGKRTSIQEYTRHGRGTKGM
                     IAIQTSARNGKVVGAVLVVPSDEIMLITTGGVLVRTRVSEIREMGRATQGVTLINIDN
                     EDTLSGVRRVAERDADDDDAGAMSPDADITGGGDDDAPSGQDGGPSDDSEDAS"
     gene            2209..3336
                     /gene="serC"
                     /locus_tag="VFR20_08675"
     CDS             2209..3336
                     /gene="serC"
                     /locus_tag="VFR20_08675"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEU0230634.1"
                     /translation="MRVWNFGAGPATLPLEVLEQAAAEMTDWHGCGMSVMEMSHRSAQ
                     YGQIRDEAEADLRELLNLPSDFAALFMQGGATAENAIVPLNLIGRGGANKADFILTGT
                     WSNKSYNEARKYGDMAVAASGGEKAVVDGVTQEPWTWYPEPRQWRVRPDASYVHLCSN
                     ETIGGLEFAQWPDLAELGAPDVPLVVDASSHMLARPFDFSRAGMVFGGAQKNAGPAGV
                     TLVMVRRDLIGHALPGCPSAFDYANVDAADSMFNTPPTYSIYIAGLVFKWLKRQGGVA
                     AMEQKNIAKSKALYGFLDQSGFYVSRVAPRDRSRMNAPFFLADESLNARFLAEAAEAG
                     LMQLKGHKSVGGMRASIYNAMPMEGVEALITFMREFERRHG"
     gene            3329..4414
                     /gene="pheA"
                     /locus_tag="VFR20_08680"
     CDS             3329..4414
                     /gene="pheA"
                     /locus_tag="VFR20_08680"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU0230635.1"
                     /translation="MDKTLQAALSPLRQRIDAIDEQILDLLNQRANTALEVGRVKLDF
                     DAEDPILKPEREAVVIRRLQALNAGPFTAEAVDAVWNQIISTCRGLESRLTVAYLGPQ
                     GSFSEQAALEHFGHAVERLRCNSFDEVFRAVEAGQADAGMVPVENSTEGAVNRTLDLL
                     LNSPLKVLGERSIKIHHCLMTRSGKLDGVKRVMAHPQALAQCQAWLTRNCPTLARGAA
                     ASNAEAARVASQDPEVAAIAGEVAAQAWGLEIVASGIQDDPQNRTRFLAVGNIQTLPS
                     GNDKTSVIMAVPNRAGAVYEMLAPLAANGVSMTRFESRPARTGQWEYYFYVDLLGHSR
                     DPAVDKALAELQKQVAFFKLLGSYPIQ"
     gene            4433..5569
                     /gene="hisC"
                     /locus_tag="VFR20_08685"
     CDS             4433..5569
                     /gene="hisC"
                     /locus_tag="VFR20_08685"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEU0230636.1"
                     /translation="MTTLDSRSDSAQADVPGYILDIAPYQAGKPIEELAREFHLDPAG
                     IVKLASNENPLGLPKSAQLAMIEASSALARYPDPNGFDLKAALSRRYGIPSDWLTLGN
                     GSNDLLELASLALLAPGAACVYSRHAFAVYRLATQARGARHIVVPVRDYGHDLDAMCA
                     AIDDDTCLVFVANPNNPTGTFQPASHIEAFLRQVHERHGEHVTVVLDEAYNEYLDPEL
                     RFDSARWVEQFSNLIVTRTFSKAYGLAGLRVGYAMAQPRLTDLLNRVRQPFNVNSLAQ
                     AAATAALDDVAFLRQSYDINKAGKKVLYEAFGKLGLEYVPSYGNFVLVRVGDAAAVNL
                     ELLKRGVIVRPVTGDGLPEWLRVSIGLPAENTRFIEALTDVLGR"
     gene            5569..6468
                     /locus_tag="VFR20_08690"
     CDS             5569..6468
                     /locus_tag="VFR20_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEU0230637.1"
                     /translation="MAQSDIGASFRVETLAIVGVGLIGGSVAAALRERGAVGRVLGVG
                     RHPHSLAQARQLGLIDEAVTLVQAAARADVILLAAPVAAIETLLAELNPHLRPDTLVS
                     DVGSTKANVVAAARKVLGARMNQFLAAHPIAGAEQTGPGAADAALFEGRTVVLTPTDE
                     ATAAARTRLTRLWEHCGARVVVMDPESHDRALASVSHLPHFLSAVFMAQVAGASDADR
                     RLGLAGTGFRDFTRIAAGSPEVWRDIFLANRSAVLAELQGFKSELDDLERALETGDGQ
                     ALFDFLERAAVARRFWGSRSGLS"
     gene            6465..7790
                     /gene="aroA"
                     /locus_tag="VFR20_08695"
     CDS             6465..7790
                     /gene="aroA"
                     /locus_tag="VFR20_08695"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU0230638.1"
                     /translation="MTDSNSLCLDPVRSASGRVDLPGSKSISNRVLLLAALCAEPTTI
                     DGLLDSDDTRVMLAALRQLGVGVESQGGHRVLVREGGAPFRTRRAELFLGNAGTAFRP
                     LAAVLSMLEGDYRLSGVPRMHERPIGDLVDALRALGAEIGYLGNPGYPPLSIGPRRPG
                     AGGTVVVRGTVSSQFLTSLLMAAPLVTATSGQPLAIAVDGELISKPYIQITLNLMARF
                     GVRVESEGWRRFTVPAGSAYTSPGGIAVEGDASTASYFMALGAIGGGPVRIQGVGASS
                     IQGDAAFADTVAAMGAKVELFPDAIVVSGAHVAAGERLRAFDEDFNLIPDAAMTAAAL
                     GMFADGPCTLRNIGSWRVKETDRIHAMHTELAKLGAQVESGPDWLRVHPVRPGAWRRA
                     AIDTYDDHRIAMCFSLAAFGPEPVTIRDPGCVAKTFPGYFSVFQGLVRP"
     gene            7787..8482
                     /gene="cmk"
                     /locus_tag="VFR20_08700"
     CDS             7787..8482
                     /gene="cmk"
                     /locus_tag="VFR20_08700"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU0230639.1"
                     /translation="MSDASTRHPVITIDGPTASGKGTVADHVARALGWHVLDSGALYR
                     LTALSVETRELDIDDTEAVADAARGLDVEFREGRVWMGGDDVTGAIRLERIGELASRI
                     AALRPVRAALLDRQRRFLRPPGLVADGRDMGTVVFPDAHLKIFLVADVDSRAERRCKQ
                     LKGKGISANLAALLEDMRARDARDRNRDVSPLVPAADAKTIDSSDLSIDETVNAVLDL
                     WSSLGAQDSGKAR"
     gene            8598..10313
                     /gene="rpsA"
                     /locus_tag="VFR20_08705"
     CDS             8598..10313
                     /gene="rpsA"
                     /locus_tag="VFR20_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU0230640.1"
                     /translation="MSTTATHAAIGGESFADLFAESVKSQDMKSGEVISAEVVRIDHN
                     FVVVNAGLKSEALIPLEEFLNDQGDVEVKEGDFVSVAIDSLENGYGDTILSRDRAKRL
                     SAWLQLEQALESGDLVTGTITGKVKGGLTVMTNGIRAFLPGSLVDLRPVKDTTPYEGK
                     TTEFKVIKLDRKRNNVVLSRRAVLEASMGEERQKLLENLSEGAIVKGVVKNITDYGAF
                     IDLGGIDGLLHITDMAWRRVRHPSEVLQVGQEVEAKVLKFDQEKSRVSLGVKQLGEDP
                     WIGLARRYPQGTRLFGKVTNLTDYGAFVEVEAGIEGLVHVSEMDWTNKNVDPRKVVTL
                     GEEVEVMVLEIDEDRRRISLGMKQCRANPWEEFSINFKRGDKVRGAIKSITDFGVFVG
                     LPGGIDGLVHLSDLSWTESGEEAVRAFKKGDELDAVVLGIDTDKERISLGIKQLEGDP
                     FNNFVATYEKGAVVPGTIKSVEAKGAVITLSVDVEGYLRASEISAGRVEDATTVLKAG
                     ESLEAMIINVDRKTRSIQLSIKARENADAAETIQRMSEASASSGTTNLGALLKAKLDQ
                     QRDDG"
     gene            10320..10649
                     /locus_tag="VFR20_08710"
     CDS             10320..10649
                     /locus_tag="VFR20_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018026132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEU0230641.1"
                     /translation="MTKSELIAILATRYPQLAARDTDYAVKTVLEAMAQALADGQRIE
                     IRGFGSFSLSSRSPRVGRNPKSGETVHVPGKLVPHFKAGKELRERVDSAYDAEIKASG
                     QSLFSVG"
     gene            10864..11193
                     /locus_tag="VFR20_08715"
     CDS             10864..11193
                     /locus_tag="VFR20_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEU0230642.1"
                     /translation="MRILAWILRLIVFVLVLMFAMNNTGPVDVNFYAGHMITGVPLIV
                     VMLVLFVIGVALGLLVALPAILRRRREARKLRRDLSRLQEQIRHPASGLPSDAVAPET
                     VAPLAPL"
     gene            11218..12429
                     /gene="lapB"
                     /locus_tag="VFR20_08720"
     CDS             11218..12429
                     /gene="lapB"
                     /locus_tag="VFR20_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEU0230643.1"
                     /translation="MDFEPWWLIFVPLLFALGWIAARVDIRQMLSETRNLPDSYFKGL
                     NFLLNEQPDRAIDAFVEVAKLDPETTELHFALGSLFRRRGEMERAIRVHQSLLSRADL
                     PQADRENAQYELAQDFLKAGMLDRAEQAFESIQGTRFAVPAMRALIRIYESEHDWPRA
                     IEAVKRLRALVDEPVPQLVHYQCEQAEAALAIPDPRAVEAALDEADNAARALHGHDGS
                     QASEARIAMLKAALARLNQAPGKELAYLESVLTIAPKFASLVAADLLECYRREGSEEH
                     GLALLKDHYLRYPSLDMFKVVFGGLRARQGYAPAWEFARDALRAHPSLLGLDRLLEAE
                     LASKDTPRQTGIAPGADLGLLRTLIHKHIQRLDRYSCRVCGFEARHFYWQCPGCSSWE
                     TYEPRRIEELK"
     gene            12426..13355
                     /gene="rfaE1"
                     /locus_tag="VFR20_08725"
     CDS             12426..13355
                     /gene="rfaE1"
                     /locus_tag="VFR20_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEU0230644.1"
                     /translation="MISFPFEAVRRLNVLVIGDVMLDRYWFGEVERISPEAPVPVVRV
                     ARREDRLGGAANVARNVVALGARATLAGVLGADEAGRQVRELARQAGIADCLVDDATS
                     PTTLKMRVLGRQQQLLRVDFEEGPGEACLSALRAAVIDLIPKHDIIVLSDYAKGALSH
                     VEQIIQAGRDAAVPVLVDPKGHLYRRYRGATLVTPNRAEMRDAVGHWQTEEELEEGAQ
                     HLRAELGLEALLVTRSEQGMTLFSEAGRQHVDAQAHEVFDVSGAGDTVLATLAVARAA
                     GLPWFDAMLLANRAGGIVVGKLGTSVVTAEELS"
     gene            13352..14344
                     /gene="rfaD"
                     /locus_tag="VFR20_08730"
     CDS             13352..14344
                     /gene="rfaD"
                     /locus_tag="VFR20_08730"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEU0230645.1"
                     /translation="MIVVTGGAGFIGSNLVRGLNRSGQSNILVVDDLTEGDKFVNLVG
                     SQIADYLHKDDFRKRVAQGQFTNIDAVLHQGACSDTTERDGRYMLDNNYRVTLELFHF
                     CQKQKIPFIYASSAAVYGSGPVYAENPANEAPLNVYGYSKYLFDQIVRRHLETLRAPV
                     VGLRYFNVYGPNEQHKGRMSSVAWHNMNQFRKEGFVRLFGGWDGYADGGQQRDFVSVD
                     DVVDVNLHFLNRTQSGIFNCGTGRAQPFNDVAGTVVNTLRAETGEAELPLADLVEQGL
                     IRYIPFPDDLKGRYQSHTQADLSQLRKAGYAAPFRDVQTGVADYVRRSLAQGRV"
BASE COUNT         2573 a         4484 c         4807 g         2680 t
ORIGIN      
        1 gtcgcgcctg cccaatctgc tggtcaacgg cagttccggc atcgcggtgg ggatggccac
       61 gaacatcccg ccgcacaatc tgtctgaagt ggtcgatggc tgcctgtact gcctgcgcaa
      121 ccccgactgc acggttgacg aactgataga gctcattccc gcgcccgact tccccaccgg
      181 cggcatcatc tatggcatgt cgggtgtccg tgaaggctat cgcaccgggc gcggccgcgt
      241 catcatgcgg gccaagacgc acttcgaaga catggaaaaa ggccatcgcc aagccatcat
      301 cgtcgacgag atcccgtacc aggtcaacaa gaagtcgctg caggaacgca tcgccgaact
      361 ggtcaatgaa aagaaggtcg agggtatctc cgacatccgc gatgaatccg acaaagacgg
      421 gatgcgtctg gtcatcgaac tgaaacgtgg cgaggtcccc gaagtcgttc tgaacaacct
      481 gttcaagaat acgcagttgc aagacacctt cggcatgaac atggtggcgt tggtcgacgg
      541 ccagccacgg ttgctgaacc tcaagcagat ggtcgagtat ttcctgttcc atcgccgcga
      601 ggtcgtcacg cgccgcaccg tgttccagtt gcgcaaggcg cgcgaacgcg ggcacgtgct
      661 cgaagggttg gcggtggctc tggccaatat cgatgatttc atcgccatca tcaaggcggc
      721 gcccacaccg ccggtggcac gccaggaact catggcgcgc agttgggatt cctccctggt
      781 acgtgaaatg ctggcacgcg ccgacgaagg cgccgagctg ggcgggcacg ccgcctaccg
      841 tccggaagac ctgcccgatg tattcggaat acagcccgac ggcctgtatc gcctgagcga
      901 gacccaggcg caggaaatcc tgaacatgcg cctgcagcgc ctgaccggcc tggagcagga
      961 caagatcgtc gcggagtaca aggacgtcat ggcggcgatc gccgatctgc tcgacatcct
     1021 ggctcgcccg gagcgcatca cgaccatcat cagcgacgaa ctggcggcca tcaaggccga
     1081 attctccacc aacgtcaaag atatccgtcg ctccgccatc gagcacaatg ccacggaact
     1141 cgacaccgaa gacctgatca cccccatgga catggtggtc acgctgtcgc aaaccggcta
     1201 tatcaagagc cagccgttgt ccgaataccg ggcacagaag cgcggggggc gcggcaagca
     1261 ggccacggcc atgaaggaaa acgactgggt cgatcaattg ttcatcgcca atacgcacga
     1321 ctatctgttg tgcttctccg accgcggccg cgtctattgg ctcaaggtgt gggaagtgcc
     1381 gcagggcacg cgtaattcgc gcgggcggcc catcgtcaac atgttcccgc tgaccgacaa
     1441 cgagaaaatc actgtcgtgc tggcgatcaa gcaattcagt gacgatcact acatcttcat
     1501 ggcgacttca cgcggcaccg tcaagaagac gccgttgtcc gatttttcca atccgcgcaa
     1561 ggcggggatc attgcggtcg atctcgatga cggcgactac ctcatcggcg ccgatctcac
     1621 agatggcgag cacgatgtca tgctgttctc cgattcgggc aaggcggtgc gcttcgatga
     1681 aaacgatgtg cgccccatgg gccgcaccgc gcgcggtgtg cggggcatga tgctcgaaga
     1741 aggccagtca gtcatttcgc tgctggtggc gcgcgatgaa tcgcaatcgg tgctcacggc
     1801 aaccgagaac ggttttggca agcgcacctc tatccaggaa tacacccggc acggccgcgg
     1861 caccaagggc atgatcgcca ttcaaacctc ggcgcgcaac ggcaaggttg tcggcgcggt
     1921 gctggtggtg ccgtcggacg agatcatgct catcaccacg gggggcgtgc tggtgcgcac
     1981 ccgggtttcc gagatccggg aaatgggccg cgcaacgcag ggtgtgacgc tgataaatat
     2041 cgataatgaa gatacattgt cgggtgtgcg ccgtgtggcg gaacgggatg ccgacgacga
     2101 tgacgccggc gccatgagtc ccgacgccga tatcacgggc gggggtgacg atgatgcgcc
     2161 atccgggcag gatggcgggc cgagcgacga ctcagaggat gcttcttgat gcgtgtctgg
     2221 aattttgggg cggggcccgc gaccctgccg ttggaagtgc tcgagcaggc ggcggctgaa
     2281 atgaccgatt ggcatggctg cggcatgtcg gtcatggaaa tgagccatcg cagcgcccag
     2341 tatgggcaaa tccgcgacga agccgaggcc gacctgcggg aattgctgaa tctgccgagc
     2401 gacttcgccg cgctcttcat gcaggggggg gcgacggccg agaacgccat cgttcctctc
     2461 aacctgattg gtcgtggcgg tgcgaacaag gcggacttca tcctcaccgg cacctggtcg
     2521 aacaagtcct acaacgaggc gcgcaagtat ggcgacatgg cggtggccgc gtccgggggc
     2581 gaaaaagccg tcgttgacgg ggtgacgcaa gagccctgga cctggtaccc cgaaccccgg
     2641 cagtggcgcg tgcggccgga cgcctcgtat gttcatttgt gcagcaacga aaccatcggc
     2701 gggctcgagt tcgcgcagtg gccggatctg gccgagcttg gcgcgccgga cgtgccgctg
     2761 gtggtggatg cgtcctcgca catgctggca cggcccttcg acttctcgcg cgcgggcatg
     2821 gtgttcggcg gggcgcagaa gaacgccggg ccggcgggtg tcacccttgt catggtgcgt
     2881 cgcgacctca tcggccatgc gttgcccggg tgcccttccg cgttcgacta tgccaatgtc
     2941 gacgcggccg actcgatgtt caacacgccg cccacgtact ccatttacat cgccggcctg
     3001 gtcttcaagt ggttgaagag gcagggcggg gtggccgcca tggagcagaa gaacatcgcc
     3061 aagtccaagg cgctgtatgg cttcctggat cagtccggtt tctacgtctc gcgcgttgcg
     3121 ccccgcgacc gttcacgcat gaacgcgccg ttctttctgg cggacgaatc gctcaatgcc
     3181 cgtttcctgg ccgaagccgc tgaagcaggc ttgatgcaac tgaaagggca caagagcgtc
     3241 ggcggcatgc gcgcctcgat ctacaacgcc atgcccatgg aaggtgtcga ggccctgatc
     3301 accttcatgc gggagttcga acgacgccat ggataaaacg ctgcaagccg cgttgtcgcc
     3361 tctgcgccaa cgcatcgatg ccatcgatga acagatcctc gatctgctca accagcgcgc
     3421 taacaccgcc ctggaggtgg gccgggtcaa gctcgatttc gatgccgaag acccgattct
     3481 caagccggag cgcgaggcgg tcgtcatccg ccggctgcag gcgctgaacg ccgggccgtt
     3541 caccgccgaa gcggtcgacg cggtgtggaa ccagatcatt tccacctgcc gggggctgga
     3601 aagccgcctg accgtggcgt atcttgggcc ccagggctcg ttttccgagc aggctgcgct
     3661 cgagcacttc ggccatgcgg tcgagcggct tcgttgcaat tccttcgacg aagtctttcg
     3721 cgcggtggag gccggccaag ccgacgccgg catggtgcca gtcgagaact ccaccgaggg
     3781 cgcggtcaat cgcacgctcg acctgttgct caattcgccg ttgaaagtgc tgggcgagcg
     3841 gtccatcaag atccatcact gcctcatgac ccggtcgggc aagctcgacg gggtgaagcg
     3901 cgtgatggcg cacccccagg ctctggcgca atgccaggcg tggctgacgc gcaactgccc
     3961 gacgctggcc cgcggcgcgg cggcgagcaa tgccgaggcg gccagggtgg cttcccaaga
     4021 ccccgaggtc gcggccatcg cgggtgaggt ggcggcccag gcgtggggac tggaaatcgt
     4081 ggcgtccggc atccaggacg acccgcagaa ccgcacccgt ttcctggcgg tgggcaacat
     4141 ccagacattg cccagcggca acgacaagac cagcgtcatc atggcggtgc cgaatcgcgc
     4201 cggcgccgtc tacgaaatgc tggccccgct ggcggccaat ggcgtgtcca tgacgcggtt
     4261 cgaatcgagg ccggcgcgca ccggccaatg ggaatactat ttctatgtcg atctgctcgg
     4321 ccacagtcgc gaccccgccg tcgacaaggc gctggcggaa ctgcagaagc aggtcgcttt
     4381 cttcaagctt ctgggctcgt atccaataca gtaaacaggc aaggcgcaac gcatgaccac
     4441 cctagactcc cgctccgatt ccgctcaggc cgacgtgccc gggtacatcc tggacatcgc
     4501 gccttatcag gcgggcaaac ccattgaaga actggcgcgg gaattccatc tggatccggc
     4561 cggcatcgtg aaactggcct cgaatgagaa tccgctgggc ctgccgaagt cggcgcaact
     4621 ggccatgatc gaagcgtcca gcgcgctggc ccggtatccg gatcccaacg ggttcgatct
     4681 gaaggcggcg ctgtcgcggc gctacggcat tccctcggac tggctcacgc tgggcaacgg
     4741 gtcgaacgac ctgctcgaac tcgcctcgct ggcgctgctc gcgccggggg ccgcctgcgt
     4801 ttattcccgg catgcgttcg cggtgtaccg cctggcgacc caggcccggg gcgcgcgcca
     4861 tatcgtggtc cccgtgcgcg actacggcca tgatctcgac gccatgtgcg cggccatcga
     4921 cgacgacacc tgtctggtgt tcgttgccaa ccccaacaac cccaccggca cgttccagcc
     4981 cgcgtcgcac atcgaagcgt tcctgcggca ggtgcatgaa cgccatggcg aacacgtcac
     5041 ggtcgtgctg gacgaggcct acaacgaata tctggatccc gagctgcgct tcgacagcgc
     5101 gcgctgggtc gagcagttct ccaacctcat cgtgacccgc actttctcca aggcctacgg
     5161 gctggcgggg ttgcgtgtgg ggtatgcgat ggcccagccg cgcctgactg atctgctcaa
     5221 ccgggtgcgc cagccgttca acgtgaacag cctggcccag gccgccgcga cggcggcgct
     5281 cgacgacgtg gcattcctgc gccaatcgta cgacatcaac aaggccggca agaaggtcct
     5341 gtatgaggcc ttcggcaagc tggggcttga atacgtgccc agctacggca atttcgtgtt
     5401 ggtgcgggtc ggggatgcgg cggccgtcaa tctcgagttg ctcaaacgag gcgtgatcgt
     5461 gcggccggtc acaggcgatg gcctgccgga atggctgcgg gtctcgatcg gcctgccggc
     5521 cgagaatacg cgtttcatcg aggcgctcac ggacgtgctc gggcggtgat ggcgcagtcc
     5581 gacatcggcg cgtcgtttcg tgtggaaacc ctggccattg tcggggtcgg gttgatcggt
     5641 ggctcggtcg cggcggcctt gcgcgagcgg ggggcggttg gtcgcgtgtt gggtgtggga
     5701 cggcacccgc actcgctggc ccaggctcgg caactcggcc tgatcgacga ggctgtgacg
     5761 ctcgtgcagg cggcggcgcg ggccgatgtg atcctgctgg cggcaccggt cgcggccatc
     5821 gaaaccctgc tggccgaact gaatccccat ctgcgcccgg atacgctcgt gtccgatgtc
     5881 ggaagcacga aggccaacgt ggtggcggcc gcgcgcaaag tcctcggggc gcgcatgaac
     5941 cagtttcttg cggcgcaccc gatcgcgggc gcggaacaga ccgggccggg agcggccgac
     6001 gcggcgctgt tcgaagggcg cacggtggtg ctgaccccca ctgacgaggc gacggccgcg
     6061 gcgcgcacgc gcctgacccg gctctgggag cactgcggcg cgcgcgtcgt ggtcatggat
     6121 cccgagtcgc acgatcgtgc tctggcatcc gtcagccacc tgccgcattt cctttcggcg
     6181 gtgttcatgg cccaggtcgc gggcgcgtcg gacgccgatc ggcgcctggg tttggccggc
     6241 acggggttcc gtgatttcac gcgcatcgcc gcgggttctc cggaagtctg gcgtgacatt
     6301 tttctggcca atcgctccgc cgtgctggct gaattgcaag gtttcaagtc tgagctcgat
     6361 gatctcgagc gggcgctgga aacgggcgat gggcaggcgt tgttcgattt cctcgagcgt
     6421 gccgccgtgg cgcgccgttt ctggggcagc cggagtggcc tgtcatgact gattcaaaca
     6481 gtttgtgtct cgacccggtg cgcagcgcgt ccggccgcgt ggatctgccc ggttccaaga
     6541 gcatctccaa ccgggtattg ttgttggcgg cgctgtgcgc cgagcccacg accatcgacg
     6601 ggctgctcga ttccgacgat acccgtgtca tgctggccgc gttgcggcaa ctgggcgtgg
     6661 gcgtcgaatc gcagggcggt catcgcgtgc tggtgcgcga gggcggcgca ccgttccgga
     6721 cccgacgggc cgaattgttc ctcggcaacg cgggcaccgc gttccggccc ctggcggcgg
     6781 tcttgtcgat gctggaggga gattatcggt tgtcgggcgt gccgcgcatg cacgagcgac
     6841 ctattggcga tctggtcgac gcgctgcggg cgctcggtgc cgagatcggc tatctgggca
     6901 acccgggcta tccgccactc tccatcggtc cgcgtcgccc gggcgccggc ggaaccgtgg
     6961 tggtgcgcgg gacggtttcc agccagttcc tgacctcgct gttgatggcc gcaccgctgg
     7021 tcaccgccac ctccggccag ccgttggcca tcgccgtgga tggcgaactg atttccaaac
     7081 cctacatcca gatcacattg aacctgatgg cgcgcttcgg tgtgcgcgtg gagagcgaag
     7141 ggtggcggcg tttcaccgtg ccggcgggct cggcctacac gtcgcccggc gggatagcgg
     7201 tcgaaggcga cgcgtccacg gcgtcctact tcatggcgct gggcgccata ggcggtggtc
     7261 cggtgcgcat tcaaggtgtc ggcgccagca gcatccaggg cgatgcggcg ttcgccgata
     7321 cggtcgcggc aatgggggcc aaggtcgagc ttttccccga tgccattgtc gtgagcggcg
     7381 cgcatgtcgc ggctggtgaa cggctgcggg cgttcgacga ggatttcaac ctgatcccgg
     7441 atgccgccat gacggcggcg gcgctgggca tgttcgccga tggtccgtgc acgctgcgca
     7501 acatcggcag ctggcgcgtg aaggaaaccg atcgtattca cgcgatgcac accgaactgg
     7561 ccaagctggg tgcgcaagtg gaatccgggc cggattggct gcgggtgcac ccggtgcgcc
     7621 ccggcgcctg gcggcgggcg gcgatcgaca cctacgacga ccaccgcatc gccatgtgct
     7681 tctcgctggc ggcgtttgga cccgaaccgg tgaccattcg cgatcccggc tgcgtcgcga
     7741 aaacgttccc cggttatttt tcggttttcc aggggttggt tcggccatga gcgatgcgtc
     7801 gacgcgacac cctgtcatca ccatcgacgg cccgacggcc tcgggcaagg gtacggtggc
     7861 cgaccacgtc gcgcgggcgc tggggtggca cgtgctcgac agcggcgcct tatatcggct
     7921 gacggcgctg tcggtggaaa cccgggaact ggacatcgac gacaccgagg ccgtcgccga
     7981 cgcggcgcgc ggactcgatg tggagttccg cgaaggtcgg gtgtggatgg gcggtgatga
     8041 cgtgacgggc gccatccgtc tcgagcgcat cggcgaactg gcctcgcgca tcgccgcgct
     8101 gcggccggtg cgcgcggccc tgctcgaccg acagcggcgt tttctccgtc ccccgggctt
     8161 ggtggcggac ggtcgcgaca tgggcaccgt ggtgtttccg gacgcgcatc tcaagatatt
     8221 cctcgtggcc gacgtcgatt cgcgtgccga gaggcgctgt aagcagttga agggaaaggg
     8281 gatttctgct aacctagcgg ccctgttgga ggatatgcgc gcgcgcgacg cacgcgatcg
     8341 caaccgggat gtttcgccgc tggtgccagc ggccgacgcc aaaacgatcg attcgtccga
     8401 tctcagtatc gacgaaacgg tcaatgcagt actcgacctc tggtcgagcc tgggtgccca
     8461 ggattccggc aaggccaggt agcccgctac ctgtcgccca tgccagggtc aggggtttct
     8521 ttttactcca ctggggcggt ttcgccgctt cggtgtgcca cttttgacag ccccgcaagg
     8581 ctgatggatc actcttaatg tccaccactg caacccatgc cgcgattggc ggcgaatcct
     8641 ttgccgacct gttcgccgag agcgtcaaga gccaggacat gaagtccggc gaagtcatct
     8701 ccgcggaagt cgtgcgcatc gaccacaact tcgtcgtggt caacgccggc ctcaagtcgg
     8761 aagcgctcat tcccctggaa gaattcctca atgaccaggg tgacgtcgag gtcaaggaag
     8821 gcgatttcgt ctccgtcgcc atcgactccc tggaaaacgg ctacggcgac accatcctgt
     8881 cgcgtgaccg cgccaagcgc ctgtcggcct ggctgcaact cgaacaggcg ctggaatccg
     8941 gcgacctggt caccggcacc atcaccggca aagtcaaggg cggcctcacc gtcatgacca
     9001 acggcatccg tgcgttcctg ccgggctcgc tggtcgacct gcgtcctgtc aaggacacca
     9061 cgccgtacga aggcaagacc accgaattca aggtcatcaa gctcgaccgc aagcgcaaca
     9121 acgtcgtgct gtcgcgccgc gccgtgctcg aggccagcat gggcgaagaa cgccagaagc
     9181 tgctcgagaa cctgtccgaa ggcgccatcg tcaagggcgt ggtcaagaac atcaccgatt
     9241 acggcgcgtt catcgacctg ggtggcatcg acggcctgct gcacatcacc gacatggcct
     9301 ggcgccgtgt gcggcatccc tccgaggtgc tgcaggtggg tcaggaagtc gaagccaagg
     9361 ttctcaagtt cgaccaggaa aagagccgcg tgtcgctggg tgtcaagcag cttggcgaag
     9421 atccctggat cggcttggcc cgccgctacc cgcaaggcac ccgcctgttc ggcaaggtca
     9481 ccaacctgac cgactacggt gcgttcgttg aagtcgaagc cggcatcgaa ggtctggtgc
     9541 acgtctccga aatggactgg accaacaaga acgtcgatcc gcgcaaggtg gtcacactgg
     9601 gcgaggaagt cgaagtcatg gtcctcgaaa tcgacgaaga ccgtcgccgc atctcgctgg
     9661 gcatgaagca gtgccgtgcc aacccgtggg aagagttctc gatcaacttc aagcgtggcg
     9721 acaaggtccg tggcgccatc aagtccatca ccgacttcgg cgtgttcgtg ggtctgcctg
     9781 gcggcatcga tggcctggtc catctgtccg acctgtcctg gacggaatcc ggcgaagaag
     9841 ccgtgcgcgc cttcaagaag ggcgacgaac tcgacgcggt ggttctgggt atcgacaccg
     9901 acaaggaacg catctccctg ggcatcaagc agctcgaagg cgatccgttc aacaacttcg
     9961 tggccaccta cgagaagggc gccgtcgttc ctggcaccat caagtcggtc gaagccaagg
    10021 gcgccgtgat cacgctgtct gttgacgtcg agggttatct gcgtgcctcc gagatctccg
    10081 ccggccgcgt cgaagacgcc acgacggtgc tcaaggctgg cgaaagcctc gaagccatga
    10141 tcatcaacgt cgatcgcaag acacgttcga tccagttgtc gatcaaggcc cgtgaaaacg
    10201 ccgatgcggc cgaaaccatt cagcgcatgt cggaagccag cgcttcgtcg ggcacgacca
    10261 acctgggcgc attgctcaag gccaagctcg accagcaacg cgacgatggt tgaacgggcg
    10321 tgactaaatc cgagctgata gcgattctgg ccactcggta cccgcaactg gcggctcgcg
    10381 acacggatta cgccgtgaag acggttctcg aggccatggc ccaggcgctg gccgacggtc
    10441 agcgcatcga gatccgtggt ttcggcagtt tttcgttgtc gtcgcgctcg ccacgtgtgg
    10501 gccgcaatcc gaaatccggt gagaccgtgc atgttccggg caagctggtt ccgcatttca
    10561 aggcgggcaa ggaattgcgc gagcgggtcg attccgccta cgacgccgaa atcaaggcgt
    10621 cgggccagtc gttgttttca gtggggtagt tctctggccg gggcggccac cgccggtgtg
    10681 gtggccgccc tgatcagcgt tggttcctca gacaagccct cgtatcgggg gcttgtctgt
    10741 tttcatgggg ctgcctgttt ttgtgggccg tcttctggcg ctgcctgatg gcatgtcggc
    10801 gtgtgtcgcg ggcgattgcc cgtacaattg aaaattaccc gaaagcaagt tggagtgctc
    10861 accatgcgta tactggcctg gatactcaga ttgatcgtgt tcgtgctggt gctgatgttc
    10921 gccatgaaca acaccggccc ggtcgatgtc aatttctatg ccggccacat gatcaccggt
    10981 gtgcccttga tcgtcgtcat gctggtgttg ttcgtcatcg gcgtggcgct aggcctgctg
    11041 gttgcgctgc cggccatcct gcgcaggcgc cgcgaagccc ggaagctgcg ccgcgacctg
    11101 agccgtcttc aagagcagat ccgccaccct gcctccggtc ttccgtcgga cgctgtggca
    11161 cccgagaccg tggcgcccct ggcgccgcta tgacgtctgg caccggaggc tgcgtccgtg
    11221 gattttgaac cctggtggct gatttttgtc ccgttgctgt tcgcgctggg gtggatagcg
    11281 gcgcgtgtgg acatccggca gatgctgtcg gaaacgcgca acctgcccga ctcctatttc
    11341 aagggcttga acttcctgct caatgaacag ccggaccgtg ccatcgatgc gtttgtcgaa
    11401 gtggcgaaac tcgatccgga aaccaccgag ctgcatttcg cgctgggcag ccttttccgg
    11461 cgccgggggg aaatggagcg ggccatccgc gtgcaccaga gcctcctgtc gcgcgccgac
    11521 ctgccgcagg cggaccgcga gaatgcccaa tacgaattgg cgcaggattt tctcaaggcg
    11581 ggcatgctgg accgcgccga gcaggcgttc gaatccatcc agggcacccg gttcgccgtg
    11641 ccggccatgc gcgcgctgat ccgcatctac gaatccgaac acgactggcc gcgtgcgatc
    11701 gaagcggtca aacgcttgcg ggcattggtc gatgagccgg tgccgcagct cgtccattac
    11761 caatgcgagc aggccgaggc ggccctggcc attccggacc cacgcgctgt cgaagccgcg
    11821 ctcgatgagg ccgacaatgc cgcgcgcgcg ttgcatggcc acgacggcag tcaggcctcg
    11881 gaggcacgca tcgcgatgct caaggcggcg ctggccaggc tcaatcaggc gccgggcaag
    11941 gaactggcct acctcgagtc cgtgctcacc atcgcaccga aattcgccag cctggtggcg
    12001 gccgatctgc tcgagtgcta tcgccgtgaa ggaagtgagg aacacggctt ggctctgctc
    12061 aaggaccatt atctgcgtta tccatcgctc gacatgttca aggttgtgtt cggcggattg
    12121 cgggcgcggc aggggtatgc cccggcctgg gagttcgcgc gggacgcctt gcgtgcccac
    12181 ccatcgcttc taggtctcga tcgcctgctc gaggccgaac tggccagcaa agacaccccc
    12241 aggcagacgg gtatcgcgcc aggcgccgat ctcgggttgc tgcgcacact tatccacaag
    12301 cacatccagc gcctcgaccg ctattcgtgt cgtgtatgcg gtttcgaggc ccggcatttc
    12361 tattggcagt gcccgggctg tagttcctgg gaaacctacg agcctcgccg catcgaggaa
    12421 ttgaaatgat ctctttcccc tttgaggccg tcaggcgcct gaacgtgctg gtgattggtg
    12481 acgtcatgct cgatcgctac tggtttggcg aggtagagcg gatttccccc gaggccccgg
    12541 tgccggtggt gcgtgtcgcg cgtcgtgaag accgtctggg cggtgccgcc aatgtggccc
    12601 gcaatgtggt cgcgctgggc gcgcgggcga cgctcgcggg cgtgctgggg gccgacgagg
    12661 ccggtcgcca agtgagggaa ctcgccaggc aggcggggat cgccgattgt ctggttgacg
    12721 acgccacctc gccgaccacg ctcaagatgc gtgtgctcgg gcgccagcag caattgctgc
    12781 gtgtcgactt cgaggagggt cccggcgagg cgtgcctgag cgcgctgcgg gccgcggtca
    12841 tcgatctcat tccgaagcac gatatcattg tgctgtccga ctatgccaaa ggggcgctca
    12901 gccacgtcga acagatcatt caggcagggc gggacgcggc ggtgccggtg ctggtggatc
    12961 ccaaggggca tttgtaccgg cgttatcgcg gggccacgct ggtgaccccg aatcgcgccg
    13021 aaatgcggga cgccgtgggc cattggcaga ccgaggagga actcgaagag ggtgcgcagc
    13081 acctgcgcgc cgaactgggc ctggaggcgc tgctggtgac ccggtccgaa caaggcatga
    13141 ctttgttttc cgaggcgggg cgtcagcacg tggatgctca ggcgcacgag gtcttcgacg
    13201 tgtcgggtgc gggtgacacg gtgctggcca cgctggccgt ggcgcgggcg gcggggctgc
    13261 cctggttcga cgccatgttg ctggccaacc gggcgggcgg tatcgtcgtg ggcaaactgg
    13321 gaacgtcggt ggtgacggcg gaggaactat catgatcgtg gtgaccgggg gcgcgggctt
    13381 catcggcagc aatctggtgc gtgggctcaa tcgctcgggc caaagcaata tcctggtggt
    13441 cgacgacctt acagagggcg acaagttcgt caatctggtg gggtcgcaga tcgccgatta
    13501 cctgcacaag gatgatttcc gcaagcgcgt ggcgcagggg cagttcacga acatagacgc
    13561 cgtgttgcac cagggggcgt gttccgacac caccgagcgc gacgggcgct acatgctgga
    13621 caacaactac cgggtaaccc tggaactgtt tcatttctgc cagaagcaaa agataccatt
    13681 catttacgcg tcgtcggccg cggtctatgg atcggggccg gtgtatgcgg aaaaccccgc
    13741 caacgaggcg ccattgaatg tctatggtta ttccaaatac ctgttcgacc agatcgtgcg
    13801 ccggcatctg gaaaccttgc gggcgcctgt ggtggggttg cgctacttca acgtctatgg
    13861 tcccaacgag cagcacaagg gtcgtatgtc ttcagtggcc tggcacaata tgaatcagtt
    13921 ccgcaaagaa ggtttcgtgc gcttgttcgg cgggtgggac gggtatgccg atggtggcca
    13981 gcaacgcgac tttgtctccg tagacgacgt ggtggatgtc aatctgcatt tcctgaatcg
    14041 cacccagtcg gggatattca attgtggcac cggccgcgcc cagcctttca acgatgtggc
    14101 tggcacggtg gtcaacacct tgcgtgcgga aaccggcgag gccgaactgc cgttggccga
    14161 cctggttgaa cagggtttga tccgctacat tccgttccct gacgatctga aaggccggta
    14221 ccaaagccat acgcaggccg acctgtcgca attacgcaag gcagggtatg cggccccgtt
    14281 ccgcgatgtc cagaccggcg tggcggacta cgtgaggcgc agtctggcgc aagggcgcgt
    14341 ttagatcctt tgggggcgtt gtgaaacagg gttgaatgca aggcgcgcgt cttcgcgacg
    14401 cgtggcgtaa cagtacggat ggacagagat gtggtgcggc gctgtgcccg cggccggtat
    14461 ccagagaaga tgaggtgtgg agcgtcgttc cacacctttt tctttttgga gacagctcat
    14521 gaatcctttt ctcgaatcca ccgt
//
LOCUS       DASTNK010000119        24711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_646795, whole genome shotgun sequence.
ACCESSION   DASTNK010000119 DASTNK010000000
VERSION     DASTNK010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 24711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24711
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646795"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..342)
                     /locus_tag="VFR20_08735"
     tRNA            complement(268..342)
                     /locus_tag="VFR20_08735"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(308..310),aa:Val,seq:cac)
     gene            complement(482..1198)
                     /gene="dnaQ"
                     /locus_tag="VFR20_08740"
     CDS             complement(482..1198)
                     /gene="dnaQ"
                     /locus_tag="VFR20_08740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEU0230646.1"
                     /translation="MRQIVLDTETTGLDPSQGHRVVELACVEMVNRSLTGNHLHLYLN
                     PDRDSDPDALRIHGLTTEFLSGHPHFEDVAQQLVDFVKDAEVIIHNAAFDTRFLTAEL
                     TRVGLPPFDQLCAKVTDSLLYARELYPGKRNSLDALCERHGISNAHRTLHGALLDSEL
                     LADVWLAMTRGQDALIMDFDDGGQASEGNGAQMDAFDPAALKVLPASASESQAHEAYL
                     NDLDKASGKPCVWRAMDAAG"
     gene            complement(1245..1697)
                     /gene="rnhA"
                     /locus_tag="VFR20_08745"
     CDS             complement(1245..1697)
                     /gene="rnhA"
                     /locus_tag="VFR20_08745"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEU0230647.1"
                     /translation="MKKEEPQVDIWTDGACKGNPGLGGWGALLRQGRHEKELCGGQTD
                     TTNNRMELLAVIEALKALKRRCVVVVHTDSQYVQKGMSEWLPNWKRRDWRTADKKPVK
                     NADLWRQLDVLVAQHDLSWRWVRGHAGDPGNERADQLANKGVEQARAG"
     gene            complement(1694..2455)
                     /locus_tag="VFR20_08750"
     CDS             complement(1694..2455)
                     /locus_tag="VFR20_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0230648.1"
                     /translation="MTQDQPLIVELADWLASPPGQYLQAWEKKQVDAMVANVFGYHAI
                     QVGLPRWDLLSANRIPGKAYTHIVHDAALDVPGMALVADPENLPFDAQSVDLLVLPHV
                     LECSSNPHQVLREAERVLVPEGRIVISGFNPWSLWGARERIPGLDPLLPVPAHLQVSL
                     ARLKDWFKLLSFEVDRGRFGCYAPHCASRKWLDHWKFMDAAGDRWWPVGGAVYVVSAV
                     KRVAGMRLVGPRWKKKKKRVHQGAVATGMRFGRKQ"
     gene            2493..3296
                     /gene="gloB"
                     /locus_tag="VFR20_08755"
     CDS             2493..3296
                     /gene="gloB"
                     /locus_tag="VFR20_08755"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEU0230649.1"
                     /translation="MLNENSGPARLLPLPALSDNYIWLLHHNGHAIVVDPGEPAPVER
                     ALEEFNLTLDAILLTHHHGDHVGGVLALRARTQAYVYGPATETLPLCDRRLKEGDLVD
                     LPNIGVRFSVLDIPGHTAGHIAYSGEINPGEPAVFCGDTLFAAGCGRLFEGTPGQMVD
                     SLGKLAVLPADTLVCCAHEYTLANLRWALQVEPGNAALHQRWEDSQAQRARGLPTLPS
                     TISLELRTNPFFRTAQKDVISAAAAHSGTPMNSPVAVFANLREWKNNFK"
     gene            3304..4677
                     /locus_tag="VFR20_08760"
     CDS             3304..4677
                     /locus_tag="VFR20_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEU0230650.1"
                     /translation="MKCLRLLILLIVAALAGCASTTTQHYAQQDTAYIAKDTSRTVDL
                     TRPPKDMWDRIRRGFAIPNLHGPLVDQWTAYYAARPEMIETMADRARKYLYYIVDQIN
                     QRGMPTELALLPFVESAYNPTALSPANASGLWQFIPSTGLHYNLKQTWWRDQRRDPIA
                     STNAALDYLNYLYDMQGDWYLALASYNMGEGAVANAMQKNASQGLPTTYLALNLPDET
                     RNYVPKLQAIKNIISDPAKYGVTLPSISNTPYFKTVRKSRDMDVAVAAHLADMSVEDF
                     KSLNASFNRPVILAEHHPVLLLPANKVEIFNANLEAYKGRLSTWELYRPRRGETYASI
                     ARHHNISVSKLREVNGISPRKRRVVAVAHAILVPTDGAGSIQLASFNVPSEDVPRTRT
                     VHEGKRIERHRINVRTHTVRHGDTLFSLARRYDTTVQKLRQLNNMRSNKLSIGEHLRV
                     PGTHVRG"
     gene            4706..5491
                     /gene="fabI"
                     /locus_tag="VFR20_08765"
     CDS             4706..5491
                     /gene="fabI"
                     /locus_tag="VFR20_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEU0230651.1"
                     /translation="MGFLEGKRILVTGVLSNRSIAYGIAQACRKQGAELALTYVGDRF
                     KERVSEFAAELGSSIVLPCDVAEDTQIEEAMTNLGKHWDGLDGLVHSIGFAPREAIAG
                     DFLEGLSREAFRIAHDISAYSFPALAKAALPLMKGRGGSVLTLTYLGAEKVVPNYNTM
                     GLAKASLEASLRYLANALGPLGLRANAISAGPIKTLAASGIKDFGSILKFVEANAPLR
                     RNVTIEDVGNVAAFLLSDLAAGITGEVTHVDAGFSTVIPGMAG"
     gene            complement(5522..6184)
                     /locus_tag="VFR20_08770"
     CDS             complement(5522..6184)
                     /locus_tag="VFR20_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEU0230652.1"
                     /translation="MAALPVLYSFRRCPYAMRARLALATSQQACELREVVLRDKPRAL
                     LEVSPKATVPVLVASDGRVVDQSLDIMLWALRQADPERWLTPNLSSMLALVAECDGFL
                     KHHLDRYKYPQRFGVPDGRVHRDQAQTWLAGLEHRLAQEGGVCGPRVALADAAIMPFV
                     RQFAHTDTEWFESRPWPSLRGWLQRWEKSALFACVMWKYPPWKPGESGSLFPPASALT
                     ET"
     gene            6423..7628
                     /locus_tag="VFR20_08775"
     CDS             6423..7628
                     /locus_tag="VFR20_08775"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU0230653.1"
                     /translation="MSLNTNQVLALGNPAIIVIFSMIFVGVWAVSGRSRHYLLLFAAT
                     FCLFAAGVTCRIIRLPASTEANAMTTAALYLASAILMVKGAATRYRIRLNTTLLVMLA
                     ITLLVIIGYFVEVRHDLILRMYILNLGVAALLFVPIKPWWGLSGQRPVDWMLFWVYTA
                     FTFSFIPRMLLYTTSISQTSQRTFTQSPIWVALELVALISIMVLGLAVLAAAVVDLVD
                     DLQKERNLDGLTQLHNRRSFEEHCLILVTERRHRPISMMMCDIDYFKSINDRYGHNAG
                     DIVLQEFGRILLECVRFDDFAARVGGEEFAVLLPDTTLEGATQFADRVRTRLSQTPFT
                     VKPLTDTAHVTQLVTASFGVVQREKNEPLTDLLLRADATLYAAKSNGRNNVTIAGELR
                     VRQSHHHCH"
     gene            7794..9065
                     /locus_tag="VFR20_08780"
     CDS             7794..9065
                     /locus_tag="VFR20_08780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0230654.1"
                     /translation="MPSHQHDTVLVIGAGIIGLCVAHALRGAGYRVTILDDQPPGSQC
                     SFGNAGALSSGSVAPLAMPGILKTAGSMLLNPDSPLHIPFHYWLRAAPWLSRFASSAR
                     PQRVLQIATALTELLSGSVAAHQTLAQAVGCRDVVHQTGQLHLYRDEQALAKDHASWN
                     LKRSHGLRMEDVDFDGIHALEPSVGADYRRGVYLPDEGWLSSPDRYTRSIAAALRAMG
                     VTFETARASKLERAGTGWQVSDGAHRWEAAHVVISAGIGSRALLAQVGPDVPLESQRG
                     YHVQAPEPCVRLSRVVVLADKKIFMTPMEGGLRIAGTVEFARPNHPPSQRRALLLERQ
                     ARAGLNGLSTSGFTTWMGHRPCLPDSLPVLGPVRGQPGLWCAFGHGHLGMTGSANTAI
                     LISRSFTGTAHPDELASFAPERFQHSGRRSV"
     gene            complement(9180..10055)
                     /locus_tag="VFR20_08785"
     CDS             complement(9180..10055)
                     /locus_tag="VFR20_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0230655.1"
                     /translation="MLDIKPLRYFITLAETRHFGRAAARLNLSQPPLSRQLVALEASL
                     GVRLVERNSRSVTLTAAGERFYADAQAILAAVRQAEENARAVAHGEAGTLAVGFTMCA
                     AYSVVPGYAKAFAATYPAVELSLREIVSNDLAGQVLSGRVDAAIMFPRVVNAGLAART
                     VVREPLCVALPRSHPRARQHKLKIAQLARDAFVMASDDVAPYLHATIVEHCRRGGFAP
                     DVRLEVRLQQTVLSLVDEAVGVALVPASMSRIHFGNVVFRPLVDAPTIELMLAWLPGN
                     RNPCLERFLDLAQEA"
     gene            10160..11101
                     /locus_tag="VFR20_08790"
     CDS             10160..11101
                     /locus_tag="VFR20_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007743357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEU0230656.1"
                     /translation="MLSILETLFPVFALIFSGFACRRRALLGPAAASEINAFVVWLAL
                     PALLFESMAHTSWRQLYQPGFTAAFALACGAVFFVTLMARLRGGRHLADASIDAIGAS
                     YPNVGYVGFPLCLIVFGKDSLVPTTIATFIVVCVLFATAIMAIEASLQGEKSLGRLAG
                     KVALSLLRNPLILAPAAGMMVAGAHITVPGFLETFIKLLAEAASPCALVSLGLFLAEK
                     RPAGDSARDSLLLTAGKLVLQPVLTWWLAAWVFSLPTALVEIAVLLAALPTGTGPYML
                     AEFYRREARITSQTILLSTVGSVVTLSVILLYIGPKG"
     gene            11374..11712
                     /locus_tag="VFR20_08795"
     CDS             11374..11712
                     /locus_tag="VFR20_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEU0230657.1"
                     /translation="MKLITAIIKPFKLDEVREALAALGIQGMTVTEVRGFGRQKGHTE
                     LYRGAEYTVDFLPKLRLEMAIADHMVEQAVESLCASAHTGKIGDGKIFITPIEQAIRI
                     RTGEAGDSAL"
     gene            11749..12975
                     /locus_tag="VFR20_08800"
     CDS             11749..12975
                     /locus_tag="VFR20_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEU0230658.1"
                     /translation="MNKADMVWMSLSTILVLLMVAPGLGLFYGGLVRSKNVLSILVQV
                     LSAFCIAIVLWFVYGYSLAFTSGGPVIGGFSRVFFSGMMNLATQKFALSGSIPEVIFA
                     AFQATFAGITCALIVGGFAERIRYSAVLVFTLIWMTLAYLPIAHMVWAPDGWLFKQGA
                     LDFAGGTVVHVNAGIAGLVGAYYLGPRIGYRRESMQPHNLPMAMMGASLLWVGWFGFN
                     AGSALGANETAALAFFNTLLATAAGVLAWTAVEWKYKGKPSLLGGISGMVAGLVGITP
                     AAGLVGPSGALVIGIVAGAVCVFGVNGLKSLLGADDTLDVFGIHGVGGITGALLTGIF
                     NAQPLGGPGLKTLAEIPHQVWVQFEGVITTLVWSGVCALIAYVIADKLCGLRVASDVE
                     REGLDITSHGETAYHR"
     gene            complement(13052..13720)
                     /locus_tag="VFR20_08805"
     CDS             complement(13052..13720)
                     /locus_tag="VFR20_08805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010109204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU0230659.1"
                     /translation="MDDAQWSVVDDYLCRTLVPADAALDGALRRSAEAGLPAINVAPN
                     QGKFLHLLARIRGARRILEVGTLGGYSTIWLGRALPPGGELVTLEVDENHARVARESL
                     REAGLSDYVSIVVGPAVSTLERMVAEGTQPFDLIFIDADKKSNPDYLAFSLKLSAPGT
                     VIVCDNVVRGGRVVDADTTDPDVRGIRTFLSRAAADPVLETTAVQTVGIKGWDGFSIS
                     VVSG"
     gene            complement(13807..14568)
                     /gene="msrA"
                     /locus_tag="VFR20_08810"
     CDS             complement(13807..14568)
                     /gene="msrA"
                     /locus_tag="VFR20_08810"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU0230660.1"
                     /translation="MSLFHIGTEGTRTRGAVRALGPWLLAVLVAFLAWQGVARAFGGF
                     GGSEAAVLLPAPTTDEPVASARTETAIFAGGCFWGVQGVFQHVRGVESAISGYAGGAA
                     STAHYETVSGGDTGHAESVEVVFDPTKVSYGTLLRIFFSVVTDPTQLNRQGPDTGTQY
                     RSALFPTDSSQRQVATAYIAQLTADHAFKRPIVTRIEKDTGFYPAEAYHQNFLVDHPR
                     YPYIVINDLPKVANMKRLFPKQYRDDPVLVASSSR"
     gene            complement(14683..16566)
                     /locus_tag="VFR20_08815"
     CDS             complement(14683..16566)
                     /locus_tag="VFR20_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006498597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein DipZ"
                     /protein_id="HEU0230661.1"
                     /translation="MWLLILAYLGGVLTIVSPCILPVLPFVFSRANQPFVRSGLPLLA
                     GMVLMFAVVASLAAVGGNWVVQANQYGRWLAMALVALFGLALLLPRLAERLTHPLVSV
                     GNRLSQAAQGGNEPRPGASFLLGIATGLLWAPCAGPILGLILTGAALQGASLGTTLLL
                     LAYAAGAATSLAAALLVGGRVFAAMKRSLGVGEWVRRGLGVAMLAGVGAIALGLDTTV
                     LAQASLASTTGIEQALVNRLSGHRRGDGQTVADAGGAGKVVGGAMRGAAQDKPEVIKT
                     NADANSMKASGVMAQSPAAMHAQPAPAEPPLPVEGRMPSLAGAVTWLNSPPLTTEGLR
                     GKVVLVDFWTYSCINCLRTLPYIEAWAKKYHDAGLVVIGVHSPEFAFERDVDNVKQAV
                     KRLGVTYPVAIDNNYAIWRAFNNEYWPAHYFVDAQGRIRHHHFGEGDYAGSERVIQQL
                     LREAGAKNVPGGLVDVRGTGVQQAPDMHDVRSPETYIGYERAERFSSGAQAHDVAQAY
                     TVSSMLPLNGWGLSGTWDVGGQRATLVNAPGRIVYRFHARDLNLVLGPRADGKPVRFK
                     VMVDGKAPGASHGADVAPDGSGMVTEQRLYQLVRQSGVIQDHTFSIEFLDAGGSAYAF
                     TFG"
     gene            complement(16573..17103)
                     /gene="msrB"
                     /locus_tag="VFR20_08820"
     CDS             complement(16573..17103)
                     /gene="msrB"
                     /locus_tag="VFR20_08820"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012493816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU0230662.1"
                     /translation="MSTRRQFLFFGAVGTAALATGLLARAGAASSESASTDAPVAAPF
                     DGPYAVTHTDAEWRRLLSPAQYEILREDGTERPYSSPLNDEHRSGTFSCAGCHQPLFS
                     SRTKFESHTGWPSFWAPLPNAVATRVDTTFGMVRTEVHCRRCGGHLGHVFHDGPRPTG
                     LRYCMDGLALMFTPKG"
     gene            17263..17979
                     /locus_tag="VFR20_08825"
     CDS             17263..17979
                     /locus_tag="VFR20_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0230663.1"
                     /translation="MDQPKRVLLVEDDTNIADLLTMHLRDERLEVVHCATGDDGLRRL
                     EQGEWDALVLDIMLPGVDGLEICRHARAMPRYTPIIIISARASEMHRIVGLELGADDY
                     LAKPFSVLELVARVKALLRRSDALARNARMDSGSLSVAGLFIDPIARTAALDARQLDL
                     TPREFDLLYFFARQPDKVFSRIDLLNAVWGYRHEGYEHTVNTHINRLRAKIEADPTRP
                     QRILTVWGHGYKFTSPEVSP"
     gene            17976..19445
                     /locus_tag="VFR20_08830"
     CDS             17976..19445
                     /locus_tag="VFR20_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0230664.1"
                     /translation="MRLTLTQRLAVVFAVLLLVCSGTSAWLQIRSNHMHEQIVIQGLS
                     RGLAEHIAQNTKLMDAGGMDTGAVRKLFGQLMVVNPSVEVYLLDAKGNIKGYDAPANR
                     MRRTRVDLAPIRRLLSGAPLPVLGDDPRSVNGRKVFSAAPLRVDGRDAGYIYVVLLGE
                     ARDQLAAQNTASNALSTTLWSIGLVALLCLVAGLTAFALITRPLRRLTDTVRHFDIEG
                     TPAAWPPAIRPGRTDGRDEIALLDNAFRQMAARLGEQWRTLTRQDQERRELIANISHD
                     IRTPLSSLHGYLETLSIKDASLDPSERRRYLGIALDQSRKVGTLAQSLFELARLEYGF
                     VSPDPESFSLTDLVQDVFQKFELSAQSKQVELKATFAPPIPTVQADLGLIERVLTNLL
                     DNALSHTPANGTVEVTLTPQASQVDVTFADSGPGVDPELQADLFRKPISLGGARRSGG
                     LGLRIVSRILQLHNCRIKLVDVPGRGAAFRFSLPISEDA"
     gene            19442..20581
                     /locus_tag="VFR20_08835"
     CDS             19442..20581
                     /locus_tag="VFR20_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEU0230665.1"
                     /translation="MTPAELSRTQAKRIWLHAQGLDAVSPFGSGPRATQTAVTHLGYV
                     QIDTINVIERCHHHILWTRIPTYRRRHLHQAQTIDKTVFEYWTHALAYIPTSDLRYFI
                     GDMQRQKKSPKSWFGSVTSTDLRKVLTRIRVDGALSIRDIDDDVLVDKDHAWASRKPS
                     KRAMQLAFHTGQLTVSERVGMLKTYELTDRHFQWDRPPRKATDSQVAGYLIDRALKSQ
                     GLISLDSICYLNAHQKPLVRKFIEDRLRKRRLIPVKVHDMESPELWTTPDTLAHNPKA
                     AQRVHILSPFDPLVIQRKRLAAFFDYRHVFEAYLPKEKRAYGYFGLPVLVDDEIVAIL
                     DIKADRERRKLLMQKWSWVGTGRPSSHKQRIEGKLHLFEKFQFAS"
     gene            complement(20594..22288)
                     /locus_tag="VFR20_08840"
     CDS             complement(20594..22288)
                     /locus_tag="VFR20_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0230666.1"
                     /translation="MASQAFQQARRFLLEHRQDYDTAYRDFRWPELKTFNWALDWFDD
                     YAHDNHDPALWIVEEGGGELKRSFAEMSARSNQVANHLRSLGVGRGDRILLMLANQVA
                     LWETLLAAMKLGAVTIPATLLLTPADIADRMTRGGVGYIVTNADQAGKFADVDASVRR
                     IAVSADAADVPAGWLRYEDALQASAEFKPDAPTQATDPMLLYFTSGTTSRPKLVQHSH
                     QSYPVGHLSTMYWIGLQPGDVHWNISSPGWAKHAWSSFFAPWNAGAAIFVYNSARFSA
                     KATLDALVKHGITTLCAPPTVWRMLIQEDMASYRVKLREVIGAGEPLNPEILGRVQKA
                     WGLTLRDGYGQTETTAQMGNSPGQPLKPGSMGRPMPGYHITLLDGDDKPASEGELSIN
                     LDPRPVGLMDGYIDDKTGGIKPMDSACYRTGDVAQVDADGYYTYVGRADDVFKSSDYR
                     ISPFELESVLIEHPAVGEAAVVPSPDALRLSVPKAFVALRAGYEPTRETAQDILRFAA
                     DKLAPYKRIRRLEFYELPKTISGKIRRVELRKAELGRVDDARRADEYWEEDFGKGG"
     gene            complement(22325..23452)
                     /locus_tag="VFR20_08845"
     CDS             complement(22325..23452)
                     /locus_tag="VFR20_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0230667.1"
                     /translation="MPSLLPNIDPDGLLEYSVVYTDRALNHMSQAFQGVMNDISRTLK
                     KVYNAKAAIVVPGSGTFGMEAVARQFATGKRCLVIRNGWFSYRWSQIFDMGGIPSDTI
                     VLKARAIESGRQAWLAPAPIEEVVASIQKNKPDMVFAPHVETASGILLPDDYLRKVAD
                     AVHAVGGMFVLDCIASGTVWVDMKAVGVDILISAPQKGWSGPACCALVMLGEAARKKI
                     DETTSTSFACDLRKWLQIMEAYEQGGHAYHATMPTDSLTVLRGVMAETERYGFEKVRE
                     EQLKLGRDVRALLRERGFKSVAAPGFEAPGVVVSYTDDDGIRSGGKFVKAGLQVAAGV
                     PLQCDEPADFKTFRIGLFGLEKLHDIDQTVARLAKALDQVA"
     gene            complement(23700..24494)
                     /gene="dsbG"
                     /locus_tag="VFR20_08850"
     CDS             complement(23700..24494)
                     /gene="dsbG"
                     /locus_tag="VFR20_08850"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HEU0230668.1"
                     /translation="MLSRPSLYRLFLGALALFLSMAGYAAETALPPSVKALQSQGLDV
                     IGEFNVGDGLRAFAGAAGDRPLAIYVTQDGSAIIGTRVGPDGKALDEGRLQDMVAKPM
                     AKRAWSALEKATWVQDGKKDAPRIIYTFSDPNCPYCHKFWEASRPWVKSGAVQLRHLV
                     VAVIRPDSATKAATILGAKDRTAELTKNELAFAEGGIKPAKSVPPAIQTILDENQELM
                     MNLGFRGTPGIVAMRSDGLLEKINGLPQPAALADLLGPNPGSPSAR"
BASE COUNT         4844 a         8127 c         7212 g         4528 t
ORIGIN      
        1 agacttcagt cgagcagttt gaacaagcga catcaggcca tcgggcagca gcatcgcgct
       61 tgcaacaacg gagaaccgcg ttcccgcctg cggaagggct ggtgcaatag accggccccc
      121 ggccagcgcc ggtggcgtcg gcgtaactgc gccacttttg tgccgaaggg cattgtgcac
      181 gaattgacga tgaccaccga aaaagaaaag gccctctggt tttcaccgta gggccttgtt
      241 ttcttaggta tttcacacta tggaacttgg tgggcggtac tgggttcgaa ccagtgaccc
      301 ctgccgtgtg aaggcagtgc tctaccactg agctaaccgc ccgatcggga aatgccgacc
      361 ggagcgcgga acacgccgcg cggtgacaaa tgctggaaag ccaggaattc tacacgaatt
      421 tccgaccctg cgcaggggca gccctgtgca ataacaagtg agcctgagac gatctgaccg
      481 ttcaaccggc ggcatccatt gcgcgccaca cgcaaggctt tcccgaagcc ttgtccagat
      541 cattcaaata tgcctcgtgc gcctgcgatt cgctggcact ggcggggagc actttcaacg
      601 cggccggatc aaacgcatcc atctgagcgc cattcccttc agaagcctgc cccccgtcat
      661 cgaagtccat gatcagggca tcctggccac gggtcatggc cagccagacg tcagccagaa
      721 gctccgaatc aagcagcgcc ccatgcaggg tgcgatgggc attggaaata ccatggcgct
      781 cgcacaaggc atccagtgaa ttgcgcttgc ctggatacag ttcgcgggcg tagagcaggg
      841 agtcagtgac tttcgcgcac aattggtcga acggaggcag ccccacgcgt gtcagttcgg
      901 ccgtgaggaa acgggtgtcg aacgccgcgt tgtggatgat gacctcggcg tccttgacga
      961 agtcgaccaa ttgctgggcg acgtcttcga aatgcggatg cccggacagg aattcggtgg
     1021 tcaggccgtg gatgcgcagg gcgtcggggt cgctgtcgcg atcaggattc aggtaaagat
     1081 gcaggtgatt gcccgtgagg ctgcgattga ccatttcgac gcaggccaat tccaccacgc
     1141 gatgtccctg ggaggggtcc aggccggtgg tttcggtatc gagcacgatc tgacgcatgg
     1201 ggtatccggt tctaatgagt gaaaactgct tgtccacacc gattttaccc ggcccgcgcc
     1261 tgctccaccc ctttgttcgc cagttgatcg gcccgttcgt tgccgggatc cccggcatgc
     1321 ccgcgcaccc aacgccacga caggtcgtgt tgcgcaacga gcacatccag ctgccgccac
     1381 agatcggcgt tcttcaccgg cttcttatcg gccgtgcgcc agtcgcgccg cttccagttc
     1441 ggaagccact ctgacatacc tttctgcacg tattgcgaat cggtgtgcac caccacgaca
     1501 cagcgccgct tcaacgcctt gagggcctcg atgacagcca ggagttccat gcgattattg
     1561 gtggtgtcgg tctgcccgcc acaaagctcc ttttcgtgcc ggccctgacg caacagggcg
     1621 ccccacccac cgaggccggg attgcccttg caggcgccgt ccgtccagat atcgacctgt
     1681 ggttcttctt tcttcattgc ttccttccga aacgcatacc cgtggcgaca gcgccctggt
     1741 gcacgcgttt ctttttcttt ttccatcgag ggccgacgag ccgcatgccc gccacccgct
     1801 tgacggccga cacgacgtag accgccccac ccactggcca ccagcggtca ccagcggcat
     1861 ccatgaactt ccagtggtcc agccattttc gggaagcgca gtgcggggcg taacagccga
     1921 aacgcccgcg gtccacctcg aacgacagca gcttgaacca gtctttcaga cgcgccaacg
     1981 aaacctggag gtgtgccggc accggcaaca gagggtccag accgggaatg cgctctcgcg
     2041 ccccccagag gctccagggg ttgaacccgg aaatcacgat ccggccttcc ggcaccagta
     2101 cacgctcggc ctcgcgcaat acctgatggg ggttggatga gcattcgagc acgtgcggca
     2161 gcaccagaag atcgacactc tgggcatcga acggcaggtt ttccggatcc gcgaccaggg
     2221 ccatgccggg tacgtccagg gcggcgtcat gcacgatgtg agtgtaggct ttgccaggaa
     2281 tgcggttggc cgagagcagg tcccaccgcg gcagaccaac ctggatcgca tggtagccaa
     2341 acacgttggc caccatggcg tccacctgtt ttttctccca ggcctgcaaa tattgccccg
     2401 gggggctagc caaccagtca gcaagctcta caataagcgg ttgatcctgc gtcacacata
     2461 cccctgcggc ccgccatggg gccgtcgcca tcatgctgaa tgaaaattcc ggcccggcgc
     2521 ggttgttgcc gcttccggcc ctgtccgaca actatatctg gttattgcac cacaatggcc
     2581 atgccatcgt ggtcgacccc ggcgagcccg cgcccgtcga gcgcgcgctc gaggaattca
     2641 acctgacgct ggacgccatt ttactgaccc accaccatgg tgaccatgtg gggggcgtct
     2701 tggcattgcg ggcccgcacc caagcgtatg tctacggccc ggcaaccgaa acactccccc
     2761 tctgcgaccg ccggctgaag gaaggcgacc tcgtcgacct gcccaatatc ggcgtacggt
     2821 tttccgttct ggacatcccc ggccatacgg ccgggcatat cgcttattcc ggcgaaatca
     2881 atcctggaga gcccgctgtt ttctgcggcg acaccttgtt cgcggccggc tgcggacggc
     2941 tgttcgaagg aacccctggg caaatggtcg attctctggg taaacttgcc gttttacctg
     3001 ccgatacact tgtttgctgc gcacacgagt acactcttgc caatttgcgc tgggcactgc
     3061 aagtggagcc gggcaatgcg gcattgcatc aacgttggga agattcccag gcgcaacgcg
     3121 ctcggggcct gcccacgctc ccctccacaa tcagcctgga attacgaacc aacccttttt
     3181 ttcgcactgc acaaaaagac gtcatcagcg cggcggcggc ccactcgggc acgccaatga
     3241 attcccccgt ggccgttttt gccaatttac gcgaatggaa gaacaacttt aaatagcctg
     3301 ccgatgaaat gcctgcgact actgattctg ttgattgtgg ctgccctggc cggatgcgcc
     3361 agcaccacga cccagcacta cgcccagcaa gacaccgctt acatcgccaa agacacctcc
     3421 cgcaccgttg acctcacccg ccctcccaag gacatgtggg accggatccg gcgcggcttc
     3481 gccatcccca acctgcacgg ccccctggtc gatcaatgga cggcttatta cgccgcccgc
     3541 cctgaaatga tcgaaaccat ggccgaccgg gcgcgcaagt acctgtacta catcgtcgac
     3601 cagatcaacc agcgcggcat gcccactgaa ctagcgctgc tgccgtttgt ggaaagcgcc
     3661 tacaacccca cggcgctttc gcccgcgaac gcctcgggcc tgtggcagtt cattcctagc
     3721 accggactgc actacaacct gaaacagacc tggtggcgcg accagcgccg cgaccccatc
     3781 gcctcgacca acgccgccct ggactacctg aactacctgt acgacatgca gggtgactgg
     3841 tacctggcgc tcgcgtcata caacatgggc gaaggcgccg tcgccaacgc catgcagaag
     3901 aacgccagcc aaggcctgcc caccacctat ctggcgctga atctgccaga cgaaacgcgc
     3961 aattacgtcc ccaagctgca agccatcaag aacatcattt ccgatcccgc gaaatacggc
     4021 gttacgctgc cttccatcag caacacccct tatttcaaga cggtgcgcaa atcgcgcgac
     4081 atggacgtcg ccgtggcggc ccatctggcc gacatgtcgg ttgaagactt caagtcactg
     4141 aacgcctcgt tcaaccgccc ggtcatcctg gccgagcacc atcccgtgct gctgttgccg
     4201 gccaacaagg tggaaatctt caatgccaac ctggaagcgt acaagggccg gctcagcacc
     4261 tgggagctct accggccgcg caggggggag acctatgcat ccatcgcccg gcaccacaac
     4321 atcagcgtga gcaagttgcg cgaggtcaat ggcatcagcc caaggaagcg gcgcgtggtg
     4381 gccgtggccc acgccatcct ggtgccaacc gacggcgcgg gcagtatcca gctggcgtca
     4441 ttcaatgtgc cgagtgaaga cgtccccaga accagaaccg ttcacgaagg caagcgtatc
     4501 gaacgacacc gcatcaacgt acgcacacac acggtgcgtc acggcgatac gctgttctcg
     4561 ctggccaggc gctacgacac gaccgtgcag aagctacgcc agctcaacaa catgagaagc
     4621 aacaaactgt ctatcggcga acacctgcgc gtgccaggca cgcacgtccg cggctgattc
     4681 acccaagcaa aaggatacac acgacatggg ttttcttgaa ggcaaacgca tcctggtcac
     4741 gggcgtcctg tctaaccggt ccatcgccta cggcatcgcg caggcatgtc gaaaacaagg
     4801 cgccgagctg gcattgacct atgtgggtga tcgtttcaag gaacgtgtga gtgagttcgc
     4861 ggcggaactc ggcagttcca tcgtactgcc ctgcgacgtg gcggaagaca cccagatcga
     4921 agaggccatg accaacctgg gcaaacactg ggatggtctg gatggcctgg ttcattccat
     4981 cggcttcgcc ccgcgcgagg ccatcgccgg cgacttcctg gaaggcctct cacgcgaagc
     5041 gttccgcatc gcccacgata tttccgccta cagcttcccg gcgctcgcca aggccgccct
     5101 gcccctgatg aaggggcgcg gcggttctgt gctcacactc acctatctgg gcgccgagaa
     5161 agtcgtcccg aactacaaca ccatgggcct ggccaaggca tcactggaag ccagcctgcg
     5221 ctatctggcc aatgcgctgg ggccgctggg cctgcgcgcc aacgccattt ccgccggccc
     5281 catcaagacg ctggccgcca gcggcatcaa ggatttcgga tccatcctga aattcgtgga
     5341 agcgaacgcg ccattgcgcc gcaacgtcac catcgaagac gtgggcaacg tggccgcgtt
     5401 cctgctgtcg gatctggctg ccggcatcac gggcgaagtc acccatgttg acgccgggtt
     5461 ctcaacagtg atcccgggca tggcgggatg attcccccgg gccggcccca ccaccgccgg
     5521 ctcaggtttc cgtcaacgcc gaagcgggcg gaaacagcga cccgctttcc ccaggcttcc
     5581 agggtgggta tttccacatc acgcacgcga acagcgcgct cttttcccac ctttgcagcc
     5641 agcccctgag cgacggccat ggccgggact cgaaccattc ggtgtccgta tgcgcgaact
     5701 ggcgcacgaa cggcatgatg gcggcgtcag ccaacgcgac acgcggacca cacaccccac
     5761 cctcttgcgc cagccggtgt tccagcccag ccaaccaggt ctgcgcctga tcgcgatgaa
     5821 cgcgaccatc aggcacgccg aatctttgcg gatacttgta acggtcgagg tggtgcttga
     5881 ggaagccatc gcattcggca accagcgcca gcatcgatga cagattcggc gtgagccacc
     5941 tttcagggtc ggcttggcgc agggcccaca acataatgtc caggctctga tccacgacac
     6001 ggccatcgct tgcgaccagc accggcactg tcgctttggg cgagacctcg agcagggcgc
     6061 gaggcttgtc tcgcagcacg acttcacgca actcgcaggc ttgttgacta gtggccagcg
     6121 ccagtcgcgc ccgcatggca tacggacagc ggcgaaacga atacaggacg ggaagcgcgg
     6181 ccataaggaa tcataaccct cccaagccgc ttatcagtaa cggtgcgaag gtcacacaaa
     6241 tgcctctaat cgacgcggat tgttgcaaaa ctgtattcga catttcgttt tgatacatct
     6301 tgtagtacca tcaaaggcat aaccccaaaa attaacttgg actcacgctt cgccacgagc
     6361 ccgcgaagta tgcggtgccg ccgttcgctg ttccccgcga tccaattttc aggaaccctg
     6421 ccttgtcgtt gaacacgaac caggtactcg ccctcgggaa ccccgcaatc atcgtcatat
     6481 tttcgatgat cttcgtcggg gtctgggcgg tgtcgggccg ctcgcggcat tacctgctgc
     6541 tgttcgccgc cacgttctgc ctgttcgccg caggcgttac ttgtcgaatc attcgcttgc
     6601 cggccagcac agaagccaat gccatgacga cggcggcact gtatctggcc agcgccatcc
     6661 tgatggtcaa aggggcggca acgcgctatc ggattcgact gaacaccacg ttgttggtca
     6721 tgctggccat cacactcctg gtgattatcg gctatttcgt cgaagtgagg catgacctca
     6781 tcctccgcat gtacatcctc aatctgggcg tggcggcact gctgttcgtc ccgatcaagc
     6841 catggtgggg gctatccggt caacgccccg tggactggat gctgttctgg gtctacaccg
     6901 ctttcacatt cagctttatt ccccgaatgc tgctgtacac cacatccatt agccagacca
     6961 gccagcgaac attcacacaa tcaccaatct gggtcgcact cgaactcgtc gccctgatct
     7021 cgatcatggt gctggggctg gctgttctcg cagccgccgt ggtggatctg gttgacgatc
     7081 ttcaaaaaga gcgcaacctc gacggcctga ctcaactgca caaccggcgc agcttcgaag
     7141 agcactgtct gattctcgtg acggaacgcc gccatcgccc catcagcatg atgatgtgcg
     7201 acatcgacta cttcaaatca atcaacgacc ggtatggcca taacgcgggc gatatcgttc
     7261 tacaggaatt cggccgcata ttgctggaat gtgtcagatt cgatgacttc gccgcacgcg
     7321 ttggcggcga ggagttcgcg gtgttgctac ctgacaccac gctggaaggg gccacgcagt
     7381 tcgccgaccg cgtgcgcacc cggctgtccc agacgccgtt caccgtcaag cctttgactg
     7441 acaccgccca cgtcacgcaa cttgtcaccg cgagcttcgg cgtcgttcaa cgcgagaaga
     7501 acgagcccct cacagacctg ctgcttcgag ccgacgccac gctgtacgcc gccaagtcca
     7561 atggccggaa caatgtgacc atcgctggcg aactgcgcgt tcggcaaagc caccatcact
     7621 gccactgacc acgcccatcc tgcgccgaag cacggcccac gagaatgccg cgacgcggta
     7681 acccacacgg cgcaccttgg ctttcacggc ttcgctcgcc aggagcggag ggcaccacca
     7741 catcggaatc caggtacagt ggccatctcg ggttcactat catgatttcc tccatgccat
     7801 cacaccagca tgacaccgtt cttgtcattg gagccggcat catcggcctg tgcgttgccc
     7861 acgcgctgcg cggcgcgggt tatcgggtca cgatcctgga tgaccagccc ccaggttcgc
     7921 aatgctcctt cggcaacgct ggcgccctgt cgtcgggctc cgtagcgcca ctggccatgc
     7981 ccggtatctt gaaaacggcc gggtcgatgc tgctgaatcc cgacagcccg ctgcatatac
     8041 ctttccacta ctggctgcgg gccgcgcctt ggctgtcgcg cttcgcctcc tcggcgcgcc
     8101 cacagcgtgt gctccagatc gccacggcgc tgacagaatt gttgtcagga tcggtggccg
     8161 cgcatcaaac acttgcacag gccgtgggat gccgggatgt cgtgcaccag accggccagt
     8221 tgcatctgta ccgggacgaa caggcactgg ccaaggacca cgcctcctgg aacctgaaac
     8281 gcagccatgg gttacgcatg gaagatgtcg atttcgacgg gatccatgcg ttggagcctt
     8341 cggttggcgc tgactaccga aggggggtgt acctgccgga cgagggctgg ctgagcagcc
     8401 ctgatcgcta cacccgatcg atcgccgccg cattgcgagc catgggcgtg acgttcgaaa
     8461 ccgcccgcgc cagcaaactc gagcgcgccg gcacgggttg gcaagtatcc gacggcgcac
     8521 accgttggga ggcggcgcat gtggtgatct ccgcgggaat cggatcccgc gcactgctgg
     8581 cccaggttgg ccccgacgtg ccgcttgaga gccagcgcgg ttatcacgtg caagcgcccg
     8641 aaccctgcgt gcgtttgtcg cgcgtggtgg tgctggccga caagaaaata ttcatgaccc
     8701 ccatggaagg cggcctgcgc attgccggca cggtggagtt cgccaggccg aatcaccctc
     8761 cttcacaacg acgcgcgtta ttgctggaac gacaggcccg tgctggtctg aacgggctga
     8821 gcacaagcgg tttcaccacc tggatggggc accggccgtg cctgccggat tcactgcccg
     8881 tcctggggcc ggtgcgaggc caacccggct tgtggtgcgc cttcggacat ggccatctgg
     8941 gtatgaccgg gtctgccaat acggccatcc tgatcagccg ctcgttcacc ggcacggctc
     9001 atcccgacga actggcctct ttcgcgcccg agcgattcca gcattcaggt cgccggagcg
     9061 tctgaatcac ccaccggcgg actctcatgg tcgagccggc tgctcctcgg cgaccggaca
     9121 ccaacatcca cgttgcctaa gaagcgacag tcggggcatc gcgatggaca tcgggcgact
     9181 caggcttctt gagccagatc caggaaccgc tcaaggcacg gattgcggtt gcccggcaac
     9241 caggccagca tcagttcgat cgtcggggcg tcgaccagcg ggcggaacac cacgttgcca
     9301 aaatgtatgc ggctcatcga ggcgggcacc agcgcaacac caaccgcctc gtcaaccagg
     9361 ctcagcacgg tttgctgcaa ccgcacttcg agccgcacat cgggcgcgaa cccgccacgc
     9421 cggcaatgct cgacaatggt ggcgtgcaaa tacggtgcca catcgtcgga agccatgacg
     9481 aacgcatccc gtgcgagctg cgcgatcttc aacttgtgtt gcctggcgcg cggatgcgaa
     9541 cgtggcagcg ccacgcatag cggctcgcga acgacggtgc gcgcggccag accggcattc
     9601 acaacgcgtg ggaacatgat ggccgcgtcg acacgccccg acaaaacctg ccccgccaga
     9661 tcattggaca cgatctcgcg cagacttagc tccactgcgg gataggtcgc cgcgaacgct
     9721 tttgcgtagc caggcaccac gctgtacgcc gcacacattg tgaacccgac cgccaacgtg
     9781 cccgcctcgc catgcgcgac agcccgggcg ttctcttccg cctgcctcac tgcggcgaga
     9841 atggcttgcg catcggcgta gaagcgctca cccgccgcgg taagcgtgac actgcgcgag
     9901 ttgcgttcga ccagacgcac acccagcgac gcctccagcg ccaccaactg gcgactgagc
     9961 ggcggctgcg acagattgag ccgggcggcc gctcgaccaa aatggcgggt ttcagccagg
    10021 gtgatgaaat agcgcagtgg cttgatgtcc agcatgccaa aacaatacct aaaaagtatc
    10081 aatctatctt aaaaataaca ttggattgca ttaaacgaca agactattct ggcgtctctc
    10141 ctattcgccg attgccgtca tgttgtccat ccttgaaacc ctgtttcctg tcttcgcgtt
    10201 gattttcagt ggctttgcgt gtcggcgtcg cgccctgctc ggcccggccg ccgcgtcgga
    10261 gatcaacgct ttcgtcgtgt ggctggcgct gccggcattg ctgttcgaaa gcatggcgca
    10321 taccagctgg cgccagttgt accagcccgg attcactgcg gcgttcgcgc tggcctgcgg
    10381 cgcggtattc ttcgtgacct tgatggcgcg cctgcgcggc ggcagacatc ttgccgacgc
    10441 gagcatcgac gccatcggcg cctcgtaccc gaatgttggc tacgtcggct tcccgctgtg
    10501 cctgattgtg ttcggaaagg acagcctggt acccaccacc atcgccacct tcatcgtcgt
    10561 gtgcgtgctg ttcgccacgg ccatcatggc catagaagcc agcctgcaag gagaaaaaag
    10621 cctgggtcga ctggctggca aagtggcctt gtcgctattg cgcaacccat tgatcctcgc
    10681 cccggcggcc ggcatgatgg tcgccggggc ccacatcacc gtgcccggat tccttgaaac
    10741 tttcatcaaa ctgctcgccg aagcggccag cccttgcgcc ttggtcagtc tgggcttgtt
    10801 cttggcggaa aagcgccccg ccggcgattc agcgcgcgac tccctgctac tcacagcagg
    10861 caagctggtg ctgcaacccg tgctgacgtg gtggctggcc gcctgggtgt tttcgctgcc
    10921 gaccgcattg gtggagatcg ctgtgctgct ggccgcgctg ccaaccggta cggggccgta
    10981 tatgctggcc gagttctacc ggcgcgaggc tcgcatcaca tctcagacca tcctgttgtc
    11041 cacggttgga tcggtcgtga cgctgtcggt gatcctgctt tacataggcc cgaaaggcta
    11101 gtgcatgcct cgccagccgt cacctcaaat ccgactcacg atagaggtct gatgccctca
    11161 tgatcgtctc acgtcaaaca gagcgtgccg acactcgaag gcttcgcgcc tgatgatcca
    11221 aattggtgcg cgcggcctcc ctgcgcacca atatcgagcg ctctgctcag cattgccatt
    11281 ccccctcccc cacagatccc gcattcacca cgggcaaaac ctggcacgcg aattgcttta
    11341 tctcggttga cacctatttc cgggaagacc gccatgaagc tgatcaccgc catcatcaaa
    11401 ccattcaaac tcgatgaagt ccgcgaggcc ctggccgcgc tgggcatcca agggatgacc
    11461 gtcaccgagg tccggggctt cgggcgccag aagggccata ccgaactcta ccggggcgcc
    11521 gaatacaccg tcgacttcct gccgaagctg cgtctggaaa tggccatcgc ggaccacatg
    11581 gtcgaacaag ccgtggaaag cctgtgcgcc tcggcccaca ccgggaagat cggcgacggc
    11641 aagatcttca tcacccccat cgagcaggcc atccgcatac gcaccgggga agccggtgat
    11701 tcggcgctct gacgcacgcc cattcattca ggaaaacagg agctacagat gaacaaagca
    11761 gacatggtct ggatgagctt gtcgacgata ctcgtcctgc tcatggtggc gcccgggctt
    11821 gggcttttct atggcgggct ggtgcgaagc aagaacgtgc tgtcgattct ggtgcaggtg
    11881 ctgtccgcat tctgcatcgc catcgtgttg tggttcgtgt acggctactc gctggccttc
    11941 acgtccggtg gcccggtgat cggcggtttc tcccgcgttt tcttcagcgg catgatgaat
    12001 ctggcgacac aaaaattcgc gttgtccggc agcattccag aagtcatatt cgccgcgttc
    12061 caggcaacgt tcgccggcat cacctgcgcg ctgatcgtgg gcggtttcgc tgaacgcatc
    12121 cgctattcgg cggttcttgt gttcacgctg atctggatga ccctggcgta tctacccatc
    12181 gcgcacatgg tctgggcccc ggacggctgg ctgttcaagc agggcgcgct ggatttcgcg
    12241 gggggcacgg tggtgcacgt caacgccggc attgccggcc tggtcggcgc ctattatctc
    12301 gggccgcgca tcgggtaccg acgcgaatcc atgcagccgc acaacctgcc catggccatg
    12361 atgggggcca gcttgctgtg ggtgggctgg ttcgggttca acgccggctc ggcgctgggg
    12421 gccaacgaaa ccgccgcgct cgcgttcttc aataccttgc tggccaccgc ggccggtgtg
    12481 ctggcctgga cggcggtgga atggaagtac aaaggcaagc cttctctgct gggcggcatt
    12541 tccggcatgg tcgcgggctt ggtcggcatc acccccgcag cgggcctcgt tgggcctagt
    12601 ggcgcactcg tcatcggcat cgtggccggt gcggtgtgtg tgttcggcgt gaacggcctg
    12661 aaatccctgc tgggcgccga cgacacgctg gatgtgttcg gcatccacgg cgtgggcggc
    12721 atcacaggcg cgctgctgac cggcatcttc aacgcccagc cgctgggcgg cccgggcctc
    12781 aagacgctgg cggagatccc gcatcaggtc tgggtgcagt tcgagggcgt catcacgaca
    12841 ctcgtctggt ccggagtgtg cgccctgatc gcctacgtca tcgccgacaa gctatgcggc
    12901 ctgcgcgtag ccagtgacgt ggaacgcgag ggcctggaca tcacctcaca cggtgaaacg
    12961 gcgtaccacc gttgatggac cgctggccgg ccgatcacat gagaggaagg ccggccagca
    13021 agaaacgaga cgcctggttg gctgggtgaa gtcagcctga cacgacggag atggagaagc
    13081 catcccagcc tttgattccc acggtctgca ccgccgtggt ctcgagaacc gggtcggccg
    13141 ccgcgcgtga cagaaaggtt ctgatacccc gcacatcggg gtctgtggtg tccgcatcaa
    13201 ccacccggcc accacgcacg acgttgtcgc aaacaatcac cgtgcccggc gccgacagct
    13261 tcagcgaaaa cgccagataa tcggggttgc tcttcttgtc cgcgtcgatg aagatcaggt
    13321 cgaacggctg agtgccctcg gccaccatgc gctcgagcgt gctgacggcc gggcccacaa
    13381 cgatcgacac ataatccgac agcccggctt cacgaagact ttcacgcgcc acacgggcgt
    13441 ggttttcgtc cacttccaac gtgaccaatt cgccgcccgg cggcagcgcc cggcccagcc
    13501 agattgtgct gtagccaccc agcgtgccaa cttccagaat gcgccgcgca ccacggatac
    13561 gtgccagcag atgcaggaac ttgccctggt tgggggcgac gttgatggcg ggcagccccg
    13621 cctcggcgct gcgccgaagc gccccatcga gcgccgcgtc cgcgggcacc agtgtgcggc
    13681 aaagataatc gtccacgact gaccactgtg catcatccat gcgcctgact ccttggtttc
    13741 aaagcaagct ccatcgtacg tcaacgacac gggcggcgtc atggcttggc atcaacaggc
    13801 tcggtgtcaa cgtgacgacg acgccaccag gaccggatcg tcgcggtact gttttggaaa
    13861 caggcgcttc atgttcgcga ccttcggcag atcgttgatg acgatgtacg ggtatcgggg
    13921 gtgatccacc aggaaattct ggtgataggc ctcggccgga tagaagccag tgtccttctc
    13981 gatgcgggtg acgatggggc gcttgaacgc atggtcagcg gtgagttgcg cgatgtatgc
    14041 cgtcgccacc tggcgctggg atgagtcggt ggggaacagc gccgaccggt actgtgtgcc
    14101 ggtgtccggg ccttgacggt tcaactgggt gggatcggtg accaccgaga agaatatgcg
    14161 caacaaggtt ccatatgaca ccttggtcgg atcgaaaacc acttccactg attccgcgtg
    14221 cccggtgtcg ccgccgctga ccgtctcgta atgcgccgtg ctcgccgcgc cgcccgcata
    14281 gccggaaatc gcgctctcca cacctcgaac gtgctggaac actccttgca cgccccagaa
    14341 gcacccgccc gcgaagatgg cggtctcggt ccgcgcggaa gcgacgggtt catcggtcgt
    14401 tggcgccggc agaagcacgg ccgcctccga gcccccaaac ccgccgaagg cgcgggccac
    14461 accctgccag gccagaaagg cgaccaacac agccagcaac catgggccca gcgcacgcac
    14521 ggcgcccctg gtgcgcgtgc cttccgttcc gatatggaat agggacatga tcagatctcc
    14581 ttgtgccccg cgtcacaatc cgaacgcggg aagcggttta ttgcattaca gtcgaatcgc
    14641 ccgccgcgtc atgtcatatg acgaggcgtg gctggaaaga catcacccaa aagtgaaggc
    14701 atacgccgaa ccacccgcat ccagaaattc aatactgaag gtgtggtcct gaatgacccc
    14761 cgactggcgc accaactggt acagacgctg ctcggtcacc atgccactgc cgtcaggcgc
    14821 cacatcggca ccatgggatg cgcctggcgc ctttccgtca accatcactt tgaaacgcac
    14881 cggcttgccg tccgcgcgcg ggcccagcac cagattcagg tcgcgggcat ggaagcgata
    14941 gacgatacgg cccggcgcgt tgaccaacgt ggcccgctgc ccgcccacgt cccaagtgcc
    15001 ggacaacccc cagccgttga gcggcagcat ggatgacacc gtgtaggcct gggccacatc
    15061 atgggcctgg gcgccggacg agaaccgttc ggcccgctcg taaccgatat acgtctcggg
    15121 cgaccgcacg tcatgcatgt ccggcgcctg ctgcacgccg gttccccgca cgtccaccag
    15181 accgccaggg acattcttcg caccggcctc ccgcagcaac tgttgaatca cccgctccga
    15241 cccggcgtaa tcaccctcgc cgaagtggtg gtggcgaatg cgcccctgcg catcgacgaa
    15301 gtaatgcgca ggccagtatt cattgttgaa ggcacgccag atggcgtagt tgttgtcgat
    15361 ggccacggga tacgtgacgc caagccgctt gaccgcctgc ttgacgttgt ccacatcgcg
    15421 ctcgaacgcg aattcgggtg aatgcacgcc aatgaccacc aggccggcat catggtattt
    15481 ctttgcccag gcttcgatat agggcaaggt acgcagacaa ttgatgcagg aatacgtcca
    15541 gaaatccacc aacaccacct tgccgcgcag accttccgtg gtcagggggg gtgaattcag
    15601 ccacgtcacg gcgccggcga gcgacggcat cctgccttca acgggaaggg ggggctccgc
    15661 gggcgcgggc tgcgcgtgca tcgcggcggg gctctgcgcc atcacaccgg acgctttcat
    15721 gctgttcgcg tccgcgttgg ttttgattac ttccggcttg tcttgcgccg caccgcgcat
    15781 cgccccgcca accaccttgc ccgctcctcc cgcatcggca acagtctggc cgtcgcctcg
    15841 gcggtggccg gacaggcgat tcaccagcgc ctgctcgatg ccggtcgtgg aggccaggga
    15901 cgcctgtgcc agaacagtgg tgtccagtcc aagagcgatg gcacccactc ccgcgagcat
    15961 ggcaaccccc aggcctcggc gcacccactc gccaacgccc aaggatcgtt tcatggccgc
    16021 gaacacccgc cccccgacca acagcgcggc ggccagagag gtcgcggctc ccgccgcgta
    16081 agcgagcagc agcagcgtcg tgcccaggct ggcaccctgc agcgcggccc cggtcaggat
    16141 caacccgaga atggggccgg cgcacggcgc ccacagcagc ccagtggcga ttcccagcag
    16201 gaacgaggcg ccgggccggg gttcgttgcc gccctgggcg gcctgggaca gccgattccc
    16261 cacgctgacc aacggatgcg tcagacgctc ggccaggcgc ggcagcagca gtgccagacc
    16321 gaacagcgca accagcgcca tggccagcca acgtccatac tgattggcct ggacgaccca
    16381 gttcccaccc accgcggcca acgacgccac cacggcgaac atcagcacca tgccggccag
    16441 caacggcagg ccactgcgca cgaacggctg attcgcgcgc gagaaaacaa agggcagcac
    16501 cggcaggatg catggactga caatggtcag cacgccaccc agataggcaa gaatgaggag
    16561 ccacatgatt attcaacctt tgggcgtgaa catcagcgcc agaccatcca tgcagtagcg
    16621 caaccccgtg ggccggggtc catcgtggaa cacatggccc aaatgcccgc cacagcgccg
    16681 gcagtgcact tcggtgcgca ccatgccgaa agtcgtatcc acccgcgtgg ccaccgcgtt
    16741 gggtagcggt gcccagaaac tcggccagcc ggtatggctc tcgaatttgg tgcgcgacga
    16801 gaacagcggc tggtgacagc cggcgcacga gaatgtcccc gaacggtgct catcgttcaa
    16861 cggactggaa tacgggcgct cggtgccatc ctcgcggagg atttcatact gggccgggct
    16921 caacaggcga cgccactcgg cgtcggtgtg cgtgacggca tagggaccgt cgaaaggggc
    16981 ggccaccggc gcatcggtcg aggccgattc actggatgcc gcacccgccc gggccagcag
    17041 gcccgtcgcc agcgctgcgg ttcccacggc gccgaaaaac aggaattgtc gtctggtaga
    17101 catcatgacc tccggatcac gaatcgttct gatgtccaaa gtgtagatcc ggggcgatcc
    17161 cggagtcctc acgcagagtt aaacaaatcg tgatgtttga atcgccgcgt tttccgtaaa
    17221 atgaaacaca ccgcgcatgt gcgcttcgac aggtttcgcc gcatggacca gcccaaacgc
    17281 gttctcctgg tcgaggacga caccaacatc gccgatctgc tcaccatgca cctgcgcgac
    17341 gagcgtctgg aggtcgtgca ctgcgccacc ggcgacgacg gcctgcgcag gctggaacaa
    17401 ggcgaatggg acgcgctggt gctggacatc atgctcccag gcgtcgatgg cctggaaatc
    17461 tgccggcacg cccgcgccat gccgcgctac acacccatca tcatcatcag cgcccgcgcc
    17521 agcgaaatgc atcgcatcgt ggggttggaa ctgggtgcgg acgactacct ggccaagccg
    17581 ttctcggtac tggagctggt cgcgcgcgtc aaggccttgc tgcgccggtc cgacgcactg
    17641 gcgcgcaacg cccgcatgga ttccggaagc ctgtcggtgg cggggctgtt catcgacccg
    17701 atcgcccgca cggccgcgct ggatgcccga caactcgatc ttacacctcg cgaattcgat
    17761 ctgctgtact tcttcgcgcg gcagccggac aaggtctttt cgcgtatcga tctgctcaac
    17821 gccgtatggg gataccggca cgagggctac gaacacacgg tcaacacaca catcaatcgc
    17881 ctgcgcgcca agatcgaggc cgatccaacc cggccacagc gtattctcac agtgtggggg
    17941 catggctaca aattcacctc accggaggtg tcgccatgag gctcaccctc acccagcgtc
    18001 tcgctgtggt cttcgccgtg ttgctgctgg tgtgcagcgg cacctctgcc tggctgcaga
    18061 tacgctcgaa ccacatgcac gagcagatcg tcatacaggg gctgtcgcgc gggctggccg
    18121 agcacattgc ccagaacacg aaactgatgg atgccggtgg catggacacc ggcgcggtac
    18181 gaaaactgtt cggtcaactg atggtcgtca atcccagtgt cgaagtgtat ttgctggacg
    18241 ccaaaggcaa catcaagggc tatgacgcgc ccgccaatcg catgcggcga acacgggttg
    18301 atctcgcccc gatacggcgc ctgctgtcgg gcgcccccct gccagtgctg ggcgacgacc
    18361 cgcgcagtgt caatggccgg aaagtattca gtgccgcgcc gctgcgcgtg gacgggcgtg
    18421 atgccggata catctatgtc gtcctgctgg gcgaggcgcg cgatcagctc gcggcacaga
    18481 acaccgccag taacgccctg agcaccacac tatggtccat cggtctggtc gcattgctgt
    18541 gcctggtggc cggcctcacg gccttcgcgc tgatcacccg ccccctgcgc cggctgacgg
    18601 acaccgtgcg ccacttcgac atcgaaggca caccggccgc gtggcctccc gcgatacgtc
    18661 ccggccggac cgacggccgc gatgaaatcg ccctgctgga caacgcgttc cgccagatgg
    18721 ccgcccgact gggcgagcaa tggcgcacgc tgacccgcca ggaccaggag cgccgggaac
    18781 tgatcgcgaa tatttcgcac gacatccgca cgccgctttc gtcgcttcac ggctacctgg
    18841 aaacgctatc gatcaaggat gcgtcgctcg acccgtccga gcgccggcga tacctgggca
    18901 tcgccctgga tcaaagccga aaagtcggca cgctggcaca gtctttgttc gaattggcgc
    18961 gactggaata tggcttcgtc tcgccggatc ctgaatcatt ttccctgacg gacctggtgc
    19021 aagacgtttt ccagaagttc gaactctccg cccaatcgaa acaggttgag ctgaaagcca
    19081 ccttcgcgcc gcccatcccg accgtgcagg cagatctggg gctgatcgag cgcgtgctga
    19141 ccaacctgct ggacaacgcg ttgtcgcaca cacccgccaa cggaaccgtg gaagtcaccc
    19201 tcacgccgca ggccagccag gtcgatgtga ccttcgccga ttccgggcct ggtgtcgacc
    19261 ccgagttgca ggccgacctg ttccgcaaac ccatcagcct gggcggcgcg cggcgcagtg
    19321 gtggcctggg cctgcgcatc gtgagccgta tcctgcaact gcacaactgc cggatcaagc
    19381 tggtggacgt tccagggcgt ggcgccgcgt tccggttttc tctgcccatc tccgaggacg
    19441 catgaccccg gccgaactct cccgcacgca ggcgaaacgt atctggctgc acgcccaagg
    19501 actggatgcc gtgtcgccat tcggctccgg tccacgggcc acacagacgg ccgtcacaca
    19561 tctgggctac gtccagatag acaccatcaa tgtcatcgag cgttgccatc atcacatatt
    19621 gtggacgcgc atacccacct atcgaaggcg gcatctgcat caagcccaga ccatcgacaa
    19681 aacagtgttc gaatactgga cgcacgcctt ggcgtacatt cccaccagcg atctgcgata
    19741 tttcatcggc gacatgcaac gccagaaaaa atcgccaaag agttggttcg gctcggtcac
    19801 cagcaccgac ctgcgcaagg tcctcacccg catccgtgtc gacggcgccc tgtccatacg
    19861 cgacatcgac gatgacgtcc tggtcgacaa agaccacgcc tgggccagcc gcaagccgtc
    19921 gaaacgcgca atgcagctcg ccttccacac cggccagctc accgtgagcg aacgcgtggg
    19981 aatgctgaaa acatatgaac tgacggacag gcactttcag tgggatcggc caccgcgcaa
    20041 agccactgac agccaggttg ccggctacct gatagaccgc gcattgaaat cacaaggcct
    20101 gatcagcctg gactcgatct gctacctcaa cgcgcaccaa aagccgctag tgcgaaagtt
    20161 catcgaagac cgattgcgca aacgacgatt gattccagtg aaagtccacg acatggaaag
    20221 ccccgaactc tggacgacac cagacaccct ggcccacaac cccaaggcgg cgcaacgggt
    20281 tcacattctt tcgccattcg accccttggt catacagcgt aaaaggctgg ccgcattctt
    20341 tgactaccgc cacgtgttcg aagcctatct gccaaaggaa aaacgcgcgt acggctactt
    20401 cgggctgccc gtcctggtcg acgacgaaat cgtcgccatc ctggacatca aggccgaccg
    20461 cgagcgccgc aagttgctga tgcaaaaatg gagctgggtc ggcacgggcc gacccagctc
    20521 acacaagcag cgcatagagg gaaagctaca cctttttgag aaattccagt tcgccagcta
    20581 gggccctagg ccttcaacca cccttcccga aatcctcttc ccaatactcg tccgcgcgcc
    20641 gggcatcatc gacgcgaccc agttccgcct tgcgcaactc aacccgacgg atcttgccgg
    20701 aaatggtctt cggcagttca tagaattcaa gccgacgaat acgcttatag ggcgccagct
    20761 tgtcggccgc gaaccgcaga atgtcctgcg cggtttcccg tgtcggttca taccccgcgc
    20821 gcagcgccac gaacgccttc ggcaccgaca ggcgcagggc atcggggctc ggcaccacag
    20881 ccgcctcgcc caccgccgga tgctcgatca gcacgctctc cagttcgaac gggctgatgc
    20941 ggtagtcgga tgacttgaac acgtcgtcgg cccggccgac atacgtgtag tagccatcgg
    21001 catccacctg ggccacgtct ccggtgcgat agcacgcgct gtccatgggt ttgatgcccc
    21061 cggtcttgtc atcgatgtag ccatccatca agcccaccgg acgtggatcg agattgatgg
    21121 acaactcccc ctcggaggcc ggcttgtcgt cgccgtcgag caacgtgatg tgatagcccg
    21181 gcatgggtcg acccatggac ccgggcttca aaggctgacc cggagaattc cccatctgcg
    21241 ccgtggtttc ggtctggccg tagccatcac gcaacgtgag gccccaagcc ttttgcacac
    21301 gcccgagaat ctctggattg agcggttcgc cggcccctat gacctcgcgc agcttcaccc
    21361 gataggacgc catgtcttcc tgaatcagca tgcgccagac cgtgggcggc gcgcacagtg
    21421 tcgtgatgcc gtgcttgacc agggcatcga gcgtggcctt cgcgctgaat cgggccgagt
    21481 tatagacgaa gatggccgcc ccggcattcc agggcgcgaa gaacgagctc caggcatgct
    21541 tggcccagcc tggcgagctg atgttccagt ggacatcgcc cggctgcagg ccgatccagt
    21601 acatggtcga caggtgccca accgggtagc tttgatggct gtgctgcacc aatttcggac
    21661 ggctggtggt gcccgaggtg aaatacagca acatgggatc tgtcgcctgg gtgggcgcat
    21721 cgggcttgaa ttccgcactg gcctgaagcg cgtcctcgta gcgcagccag cccgccggca
    21781 cgtcggcggc atcggccgat acggcgatgc ggcgcacgct ggcatcgaca tccgcgaact
    21841 tgcctgcctg atcggcattg gtgacgatat agccaacccc accccgcgtc atgcggtcgg
    21901 cgatatccgc gggggtcaat agcaaagtgg cgggaatcgt caccgcgccc agcttcatgg
    21961 ccgccagcag cgtttcccac aacgccacct ggttcgccag catcaacagg atgcgatcgc
    22021 cgcgcccgac acccaggctg cgcaaatggt tcgccacctg gttcgaccgc gcggacattt
    22081 cagcgaagga gcgcttcagt tcaccgccgc cctcttccac gatccacaag gcgggatcat
    22141 gattgtcatg ggcgtaatcg tcgaaccagt ccaacgccca gttgaacgtc ttgagttcag
    22201 gccatcggaa atcccggtaa gccgtgtcgt agtcttgacg atgctcgagc agaaaccgac
    22261 gggcctgctg gaacgcttgt gatgccatgg gggtctcctt cctggttggc cagactattg
    22321 aagttcaagc gacctgatcc agcgccttgg ccagccgcgc cacggtctgg tcgatgtcat
    22381 gcagtttttc aagaccgaac aagccaatgc ggaacgtctt gaagtcagcg ggctcgtcac
    22441 actgcaacgg cacccccgcc gcgacctgca ggccggcttt gacgaatttt ccaccggagc
    22501 gaatgccatc gtcatcggtg tagctgacaa ccacgcccgg cgcctcgaag cctggcgccg
    22561 ccacgctctt gaagccgcgt tcacgcagca gcgcgcgcac gtcgcgcccc agcttcagtt
    22621 gctcttcgcg caccttctcg aaaccatagc gttccgtttc tgccatcacg ccgcgaagga
    22681 ctgtgagcga atcggtgggc atggtggcat ggtaggcgtg gccgccctgt tcataggctt
    22741 ccatgatctg cagccatttg cgcaaatcac acgcgaagct ggtgctggtc gtctcgtcga
    22801 tcttcttgcg cgccgcctcg cccaacatca ccagggcgca gcatgccgga ccgctccaac
    22861 ccttctgcgg cgcactgatc aagatgtcaa cgcccacggc tttcatgtca acccataccg
    22921 tgcccgacgc gatgcaatcc agcacgaaca tcccgcccac cgcgtgcacc gcatcggcca
    22981 ccttgcgcag gtagtcgtcg ggcagcaaga tgcccgacgc ggtttcgacg tggggcgcga
    23041 acaccatgtc gggtttgttc ttctggatgg acgccaccac ttcttcgata ggggccggcg
    23101 cgagccacgc ctgccggccg gactcgattg cgcgagcctt gagcacgatg gtgtcggacg
    23161 ggataccgcc catgtcgaag atctggctcc agcgatagct gaaccagccg ttgcgtatga
    23221 ccaggcagcg cttgccagtg gcgaactgcc gcgccaccgc ctccatgcca aacgtgccac
    23281 tgcccggcac cacgatggcc gctttggcgt tgtagacctt tttcagggtg cgggagatgt
    23341 cgttcatgac cccttgaaag gcctgcgaca tgtgattcaa cgcacggtcc gtgtagacca
    23401 cggaatattc gagaagcccg tcgggatcaa tattgggtag taggctgggc acgcttgtct
    23461 ccggagaaga aaaggaatca gccgggtcct gagcacgaga aaccgaggaa accaacccct
    23521 cgcacatcgc cggcccgaca cacaatgtct gggtccgatt ttagaccatg ccctgtggac
    23581 gaagccgggt atttttttcc acaccctttt ggtgtccttt tcatgcttac aactgatcta
    23641 cattgtatct cttatcttca agccgaagtg gaatcttcaa ataccgcaca ccgttggact
    23701 cagcgagcgg aaggcgaacc aggattcggc cccaacagat ccgcgagcgc ggccggctgc
    23761 ggcaggccgt tgattttttc cagcaaaccg tcgctgcgca tcgcaacgat acccggtgtg
    23821 ccgcgaaacc ccagattcat catcagttcc tggttctcgt ccaggatggt ctgaatggcg
    23881 ggcggcaccg atttcgctgg cttgatgccg ccttcggcaa aagccaattc attttttgtg
    23941 agttcggcgg tgcggtcttt ggcgccgaga atggttgcgg ccttcgtggc gctgtcaggg
    24001 cgaatcactg cgacgaccaa gtggcgcagt tgcacagcgc cggatttcac ccaggggcgg
    24061 gacgcctccc agaacttgtg gcagtacgga caattggggt cgctgaatgt gtaaatgatt
    24121 cgcggcgcgt ctttcttgcc gtcctgcacc caagttgctt tttccagcgc ggaccaggcg
    24181 cgcttggcca tcggctttgc aaccatgtct tgcagacggc cttcgtcaag cgccttgcca
    24241 tccggcccca cacgcgtgcc gatgatggcg ctgccgtctt gggtgacgta gattgccagc
    24301 ggacgatctc cggccgcgcc ggcaaacgcg cgcaagccgt cgcccacatt gaactcaccg
    24361 atgacgtcca gcccttgtga ctgaagcgcc ttgacggacg gcgggagcgc cgtttcagcc
    24421 gcgtaacccg ccatcgacaa gaacagggcc aacgccccga gaaacaaacg atatagactg
    24481 ggacgtgaca acatgatgat tcctgagctt ggagtccccg gaacaaatca ccttgttccg
    24541 gggactcctt gaaaagatag cggtgaaccc cccgcaatac ctctttatag tgcttttcag
    24601 gccggcatgg tggacccgtc catcacgcca gcgccgctgg caacggacgt cggcgattca
    24661 tcaggatgga tgattcggcc tggaacccga cgctgtgaat ttcttcagga a
//
LOCUS       DASTNK010000120         6854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1015831, whole genome shotgun sequence.
ACCESSION   DASTNK010000120 DASTNK010000000
VERSION     DASTNK010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015831"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="VFR20_08855"
     CDS             <1..146
                     /locus_tag="VFR20_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HEU0230669.1"
                     /translation="FGLQSGGRTESILLSMPPAASWRYCWSPEPGSPEARLADYLKPR
                     EWL"
     gene            137..739
                     /gene="nadD"
                     /locus_tag="VFR20_08860"
     CDS             137..739
                     /gene="nadD"
                     /locus_tag="VFR20_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEU0230670.1"
                     /translation="MALKKTGLLGGSFDPVHVAHIALADAACRDAGLDHVELIPVADP
                     WQRAPLSASPAQRLDMLRLAIQGHSKLSVNPVEISRGGKTYTLDTVNELPATAEYYWI
                     LGADQLANFCSWHGWRDIARRIHLLVAQRPGAPLAAPALLADHLVACGRQLFELSFPP
                     MPISASEIRRRLATGESVHGLLSPEVERYIEENGLYHAPE"
     gene            776..1168
                     /gene="rsfS"
                     /locus_tag="VFR20_08865"
     CDS             776..1168
                     /gene="rsfS"
                     /locus_tag="VFR20_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU0230671.1"
                     /translation="MDIQKLQRLVIDALEDVKAQDIKVFNTTHITSLFERVVIASGTS
                     NRQTRALAHSVMEQARAQNVTVVACEGEDSGEWVLVDLGDLVVHCMQPAIRHYYNLEE
                     LWGDKPVRVKLLPQSTQPTPVGYADNNG"
     gene            1165..1635
                     /gene="rlmH"
                     /locus_tag="VFR20_08870"
     CDS             1165..1635
                     /gene="rlmH"
                     /locus_tag="VFR20_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU0230672.1"
                     /translation="MKLIVVAVGTRMPDWVETAWNEYAKRMPADCLLEIREIKPEPRT
                     SGKTPAQMMQAEARRIETAIPAHAHTVALDEHGHDVTTRQLADALQDWRTLGHDIAFL
                     VGGPDGLDADLKHRCRQRIRLSSLTLPHPMVRALLAEQLYRAWTITAGHPYHRA"
     gene            1674..2279
                     /locus_tag="VFR20_08875"
     CDS             1674..2279
                     /locus_tag="VFR20_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEU0230673.1"
                     /translation="MTDPALLYLASASPRRHDILSQMGIPHHVLSVPPPPGEDEPIHD
                     GESAADYVQRTARDKARHALHWIGSQFLPPLPILSADTTVILDGNILGKPRNRDDAAA
                     TLKRLSGREHEVRTAIAIVHSGRLYEDVSVTRVRFRDLAEHDIQTYCATGEPMGKAGS
                     YAIQGWAGRFVAHLDGSYTGVMGLPMYETDQLLIKAGVLTS"
     gene            complement(2349..2939)
                     /locus_tag="VFR20_08880"
     CDS             complement(2349..2939)
                     /locus_tag="VFR20_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417231.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HEU0230674.1"
                     /translation="MKTLIENNGFTNDTLDWKAWVDVVDCDTATPEQIEVLEMSHPQA
                     KSSDYYLALAHQPEILRQRSEAYNAIMYAPGGLPRADRELGALTVSRVNGCVYCCSVH
                     AQRYEQMAKRNDTVAQVFDEPRTAGTSDREKAVVAFSIKLTESPQDVGATDIAALKDA
                     GLSEAEILDLLNATSIFAWANRLMMNLGEAVRRSRS"
     gene            complement(2936..3484)
                     /locus_tag="VFR20_08885"
     CDS             complement(2936..3484)
                     /locus_tag="VFR20_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain protein"
                     /protein_id="HEU0230675.1"
                     /translation="MQILNGAPHDTPLGDVASDDLMDAVTGLIPSDPLYAVRHAREKV
                     AVAMQKSYDVFFDATARGLSLSERFSVALYASVLSRSEALAGHYEQALLGQGVEPGVL
                     DAMLTDRLDAVSNNRLRIMLGFTRKLIVKPVEGDAAEIERLRSAGVATPDIVTLAQLI
                     AFLSYQIRVAAGLRAMKELESQ"
     gene            complement(3549..4838)
                     /locus_tag="VFR20_08890"
     CDS             complement(3549..4838)
                     /locus_tag="VFR20_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/bile acid-CoA:amino acid
                     N-acyltransferase family protein"
                     /protein_id="HEU0230676.1"
                     /translation="MTQPSLTVEPADALVDVPRRIRLTGLAPHARVTLGANTLRAGGV
                     AWNSSARFQADAGGVVDASEQAPLDGDYDSVSAMGLVWSQQPATVGSINVFPDNLLEP
                     VETRLVADTGDQRVEATLVQRFAAPGVVREEIREDGLVGTLFRPATPGPHPAVMILNG
                     SGGGMNEPRAALYASHGFVALALAYFKAPGLSNYISNTPLEYFKRGLDWMRATQKPAK
                     GFVALNGQSRGGELVLLLASLYPDDVSAVVAYVPGAVVHGGQNAADPAVGRDGPAWLL
                     DGKPVAHIWNDNRTASWAAYDAGQRHEVSIRTALQDAEAVARARIPVERIRAPIILLS
                     GTDDGAWPSSEYSRMMMDSLRANHHSYDYQWVDTPDAGHAIVFPYVPTTQITHMHPVT
                     GRLFTGGGAPGPNAVSNENSWRGIKAFLTRAVENHTG"
     gene            complement(4847..6418)
                     /locus_tag="VFR20_08895"
     CDS             complement(4847..6418)
                     /locus_tag="VFR20_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0230677.1"
                     /translation="MKRTLASLLAVGALALGAQAGAQTLTIGFADPLSSLDPQLNNFA
                     GDRSSDLHFFSMLLQKRGNELTPDLAESWKVVDPDTWEFKLRPGVKWTDGQPLRPEDV
                     IFSIKRAGHVPGSVAPFSGFVRMIASVEQKGNDTIVVKTKTPSPNLPLNLASIHIVSK
                     HVGENAKPEDYNSGKALIGTGPYKLVSYTPGDRVVMTRNDGYWAGPQPWEKVVYRYIS
                     NPASRTAALLSGDVDVIDKVSVSDLPRLKAAKGVSVYAYKGLRVMLLQPSFRKGPNPY
                     LRDNSGKELPENPLLDVRVRKALSLAIDRQAIASRIMQDTVTVANQWMPADTFGYNPA
                     LKDIPYDPAKARQLLKEAGFPDGFQMTLHVPSDRYPLAPQTAQAAAQFWARIGVKTKV
                     EVVPWAVYAGQARKNDYAMSMIAWGNGTGEASYALVNILATVSPQKGLGASNWGHYSS
                     ARVDDDLRRATAEFDDQKREAILRESAVAVRDDVGIIPLFHYKNIWAARKGLKVVPWT
                     SDRTVAMMVTPVDGK"
     gene            complement(6466..>6854)
                     /locus_tag="VFR20_08900"
     CDS             complement(6466..>6854)
                     /locus_tag="VFR20_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEU0230678.1"
                     /translation="NVFMDLREQLNLTYLFISHDLGVVEHLSDRVVVMYLGRVVETAP
                     VEELFRHPAHPYTRALLAEVPRIDVRGKRFAAIQGEIPSPLNPPSGCHFHPRCPHAMA
                     RCKTEAPILKPLGSGHLSACHLDAGR"
BASE COUNT         1294 a         2268 c         2043 g         1249 t
ORIGIN      
        1 tgttcggcct gcaatcaggc ggacgcaccg aatcgatcct gttgtcgatg ccgccggccg
       61 cctcctggcg ttattgctgg tcgcccgagc cgggttctcc ggaagcacgg cttgccgact
      121 acctgaagcc acgcgaatgg ctttgaaaaa gaccggcctg ctggggggca gcttcgatcc
      181 ggttcacgtg gcccatatcg cgctcgccga cgcggcgtgt cgcgacgcgg ggctggatca
      241 cgtcgaactc atccccgtgg ccgacccctg gcaacgcgcg ccactgagcg ccagccccgc
      301 gcaacgattg gacatgttac gcctggccat tcagggccac tccaagctgt cggtcaaccc
      361 cgtcgaaatc tcgcgggggg gcaagaccta tacactggac accgtgaatg aactgccggc
      421 caccgccgaa tactactgga tactaggcgc cgatcaactg gccaacttct gctcgtggca
      481 cggctggcgc gacatcgccc ggcgcatcca tctgctcgtc gcacaacgcc ccggcgcccc
      541 actggccgcg ccagcactgc tcgcggacca tctggtggca tgcggccgcc aacttttcga
      601 actatcgttt ccccccatgc ccatctcggc ctccgagatt cgccgcagac tggccaccgg
      661 agagtcggtg catgggttgc tgagccccga ggtggaacgt tacatcgaag aaaacggcct
      721 gtaccacgcc ccggaataat gcctccgccc ccctcctcac gttatagttg aagacatgga
      781 catacaaaaa ttgcaacgcc tcgtcatcga tgccctggaa gacgtcaagg cccaggacat
      841 caaggtgttc aataccacac acatcaccag cctcttcgag cgcgtggtca tcgccagtgg
      901 cacctccaac cgccagacgc gcgccctggc ccacagcgtc atggaacaag cccgggcgca
      961 aaacgtcacc gtcgtggcct gcgagggcga ggattcaggc gaatgggtac tggtcgacct
     1021 gggtgatctg gtggtgcatt gcatgcaacc ggcgatccgc cactactaca acctcgaaga
     1081 actctggggt gacaagcccg tgcgcgtcaa gctgctgccc cagtccacgc agccgacccc
     1141 ggtcggctac gcggacaaca acggatgaaa ctgattgtcg tcgccgtcgg cacgcgcatg
     1201 cccgattggg tggagaccgc ctggaacgaa tacgccaagc gcatgccggc cgactgcctg
     1261 ctggagatcc gggaaatcaa gcccgagccc cgcacgtcgg gcaagacccc ggcacagatg
     1321 atgcaggccg aagcgcggcg catcgaaacg gccatccccg cccatgcgca taccgtggcg
     1381 ctcgatgagc acggccacga cgtcacgaca cggcagcttg ccgacgcgtt gcaagactgg
     1441 cgcacgctgg ggcatgatat tgcttttctg gttggtggcc ccgatgggct ggatgccgac
     1501 ctcaaacacc gctgtaggca gcggatccgg ctgtcgtccc tgaccttgcc gcaccccatg
     1561 gtgcgcgcgc tcctggccga acagctttac cgcgcctgga ccatcaccgc cggacacccg
     1621 taccatcggg cctaagcggc cggctccccg cgccccaacc cttgcgccac gccatgactg
     1681 atcccgctct tttatatctg gcctcggcca gcccacgccg ccacgacatt ctgtcgcaaa
     1741 tgggcatccc gcaccacgtc ctgagcgtcc ctcccccacc cggagaggac gaacccatac
     1801 acgatggaga atccgccgcc gactacgtgc aacgcaccgc gcgcgacaag gcacggcatg
     1861 cactgcactg gatcggatca cagttcctgc cgcctcttcc catactgagc gccgacacca
     1921 cggtgattct ggacgggaac atactgggca agccgcgcaa ccgcgacgac gccgccgcca
     1981 cactgaagag gctttccggc cgcgagcacg aagtgcgcac cgccatcgcc atcgtccatt
     2041 ccgggcgcct gtatgaagac gtgtccgtca cccgggtgcg cttccgggac ctggccgagc
     2101 acgacatcca gacctattgc gccactggtg aaccaatggg caaggcgggc agctacgcca
     2161 tccagggatg ggccggccgg ttcgtggcgc acctggacgg cagctatacc ggcgtcatgg
     2221 gattgcccat gtacgaaacc gaccagttgc tgatcaaggc tggcgtgctg acgagctgaa
     2281 cgcgctcgcg ctcaggggaa cgctcgtgat ggccttcgcc accacgaggc acgccatgcc
     2341 gacgcgccct acgatcggct gcgccgcacc gcctcgccta gattcatcat gagccggttg
     2401 gcccatgcga agatcgacgt ggcgttgagc aagtcgagga tctcggcttc ggaaagaccg
     2461 gcatccttga gcgcggcgat atcggtggcg cccacgtcct ggggggactc ggtaagcttg
     2521 atggagaacg ccaccacggc cttctcgcga tctgacgtgc cggcggtgcg tggctcgtca
     2581 aacacctgtg ccacggtgtc gttgcgcttg gccatctgct cgtagcgctg ggcgtgcaca
     2641 gaacagcaat acacgcatcc gttgacgcgc gacaccgtca aggcgcccaa ttctcgatca
     2701 gcgcgtggca agccgccagg cgcatacatg atcgcgttat aggcttcgga acgctgccgc
     2761 agtatttctg gttgatgcgc cagggccaga taataatcgg acgacttggc ctggggatgg
     2821 ctcatctcga gcacttcgat ctgctccggc gtggcggtgt cgcaatccac gacgtccacc
     2881 catgccttcc agtccagcgt atcgttggtg aaaccgttgt tttcgatcag agtcttcatt
     2941 gggattccag ctccttcatg gcacgcagtc cggccgccac ccggatctga taggaaagaa
     3001 acgcgatcaa ttgcgcaagc gtcacgatat ccggcgtggc cacccccgcg ctgcgcagcc
     3061 gctcgatctc ggcggcatca ccttccacgg gcttgacgat caacttgcgc gtgaaaccca
     3121 gcatgatgcg cagacgatta ttcgacacag cgtctagcct gtcggtcagc atggcatcca
     3181 gtacacctgg ctccacgcct tgcccgagca gcgcctgttc ataatgcccg gccagtgctt
     3241 ccgatcggga cagcacgctt gcgtagagcg cgaccgaaaa tcgctccgac aagctcagac
     3301 cccgcgcggt ggcatcaaag aacacgtcgt aactcttctg catggccaca gccacttttt
     3361 cccttgcgtg ccgcacggca tacaagggat cggacgggat caatcctgtg acggcatcca
     3421 tgaggtcatc cgaggcgaca tcacccaacg gggtgtcgtg tggggctcca ttcaagatct
     3481 gcattgtttc tcctctataa tgtgccgacg gctggaaccg cctggctgaa ttccagctcg
     3541 agcccgtatc agcccgtgtg attttccacg gcgcgcgtca agaacgcctt gatgccacgc
     3601 cacgagtttt cgttggacac cgcattgggg ccaggggcgc caccaccggt gaacaagcgc
     3661 cctgtcactg gatgcatgtg cgtgatctga gtggtgggaa cgtagggaaa gacaatggca
     3721 tgcccggcat cgggtgtatc cacccactga taatcataag agtggtggtt cgcccgcagg
     3781 gaatccatca tcatccggct gtactcgctg gatggccagg ctccgtcgtc ggtgcccgac
     3841 agcaggatga tgggggcgcg aatgcgctcg accggtatgc gcgcgcgggc gaccgcttcg
     3901 gcatcctgca gcgctgtccg gatcgacact tcgtgacgct gcccggcatc gtaggcggcc
     3961 caactggccg ttcgattgtc gttccagatg tgcgccacag gtttgccatc cagcagccag
     4021 gccgggccat cacggccgac ggcgggatcc gccgcattct ggccaccgtg caccaccgcg
     4081 cccggcacgt aggcgacaac cgccgataca tcatccggat acagcgaagc cagcagcagc
     4141 accagttccc ccccgcgaga ctgcccattc agggccacga atcctttggc gggtttctgt
     4201 gtcgcgcgca tccagtccag gccgcgcttg aaatattcga gcggcgtgtt ggagatgtag
     4261 ttggaaagcc ctggagcctt gaaatacgcc aacgccagcg ccacaaagcc gtgcgatgca
     4321 tacagcgcgg cgcgcggctc gttcatgccg ccaccagacc cgttcagtat catcactgca
     4381 ggatggggcc cgggcgtggc cggacggaac agcgtaccga ccagaccgtc ttcccgaatt
     4441 tcctcgcgta cgacgcctgg cgccgcgaag cgctgcacta gggtggcttc gacacgttga
     4501 tcgcccgtat cggccaccaa ccgtgtctcc acgggctcca gcagattgtc cggaaagacg
     4561 ttgatcgacc ctactgtggc tggctgctga gaccaaacca gccccatcgc cgacacactg
     4621 tcgtagtctc cgtccaacgg cgcttgctcg ctggcgtcga caacaccccc ggcatcggcc
     4681 tggaaccgtg cggagctgtt ccaggccacc ccgccagcgc ggagcgtgtt cgcgccaagc
     4741 gtcacccggg catggggcgc gagtcccgtc aggcgtatgc ggcgtggcac atcgaccaac
     4801 gcatcagcgg gttccacagt gaggctgggt tgagtcatga cgcgtcctac tttccgtcaa
     4861 ccggggtgac catcatggcg accgttcgat cactggtcca cggcaccact ttcaggccct
     4921 tgcgcgcggc ccagatgttc ttgtagtgga acaacggaat gatacccacg tcatcgcgca
     4981 cggcgaccgc cgattcgcgc aggatggctt cacgcttctg gtcgtcgaac tcggccgtcg
     5041 cgcggcgcag atcgtcgtcc accctggcac tgctgtaatg gccccagttc gaggcgccca
     5101 accccttctg cggcgacacg gtggctagaa tgttcaccag cgcatagctg gcttcacccg
     5161 tgccattgcc ccaggcgatc atgctcatcg catagtcgtt cttgcgggcc tgccccgcat
     5221 acacagccca tggcacgacc tcgaccttgg tcttcacgcc gatgcgggcc cagaactggg
     5281 ccgcggcctg ggcggtttgc ggggcgagcg gatagcggtc cgacggcacg tgcagggtca
     5341 tctggaagcc atcggggaag cccgcttcct tcagcaactg tctggccttg gcgggatcgt
     5401 aggggatgtc cttcagggcc gggttgtagc cgaaggtgtc ggccggcatc cattggttgg
     5461 ccacggtgac cgtgtcctgc atgatgcggc tggcaatggc ctgccgatcg atggccaagg
     5521 acagcgcctt gcgcacacgc acatccaaca gggggttctc gggcaattcc ttgccgctgt
     5581 tatccctcag gtacggattc ggccccttgc ggaagctggg ttgcaacagc atgacgcgca
     5641 accccttgta cgcgtagacg gacacaccct tggcggcctt cagcctgggc agatcggaca
     5701 ctgatacctt gtcgatcaca tccacatcac ccgacagcag cgcggccgtg cgcgatgccg
     5761 gattggatat gtagcgataa acgactttct cccagggctg cggccccgcc cagtaaccgt
     5821 cgttgcgagt catgacgaca cgatcgccgg gtgtgtagga caccagtttg tacgggccgg
     5881 ttccgatcag cgccttgccg ctgttgtagt cttccggttt cgcattctct cccacgtgtt
     5941 tgctgacaat atggatcgac gcgagattca gcggcaggtt gggactgggc gttttcgtct
     6001 tgacgacgat ggtgtcattg cccttttgct cgacgctggc aatcatgcgt acgaacccac
     6061 tgaacggggc gacggagccc ggcacatgcc cggcccgctt gatcgagaaa atgacgtctt
     6121 cgggccgcaa cggttggccg tccgtccatt tgacgcccgg acgaagcttg aattcccatg
     6181 tgtccggatc caccaccttc cagctttcag ccagatcggg cgtcagttcg ttgccgcgct
     6241 tctgcagcaa catgctgaag aaatgcagat ccgacgagcg atcacccgcg aaattgttca
     6301 gttgcggatc cagtgacgac aggggatcgg cgaacccgat cgtcaaggtc tgcgcgccag
     6361 cctgggcgcc gagggccagt gcgcccacgg ccagaaggct tgccaacgtt ctcttcatgt
     6421 gtgtctccct tgttgaaatg aatggcttga tatgcatgga tcgggtcaac gccccgcgtc
     6481 gaggtgacaa gcgctcaaat gaccgcttcc caggggcttg agaatggggg cttccgtctt
     6541 gcagcgcgcc atggcatgcg ggcagcgtgg atgaaaatgg caacctgagg gcggattcag
     6601 cgggctggga atctcgccct ggatggccgc gaagcgcttg ccgcgcacat cgatgcgcgg
     6661 cacttcggcc agcagcgccc gggtgtacgg gtgagcggga tgccggaata gctcttccac
     6721 cggggcggtt tcgaccacac gccccagata catcaccacg acccggtcgg acaaatgctc
     6781 gaccacaccc agatcgtggc tgatgaacag ataggtcagg ttcaactgct cgcgcagatc
     6841 catgaacacg ttca
//
LOCUS       DASTNK010000121         5088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_91762, whole genome shotgun sequence.
ACCESSION   DASTNK010000121 DASTNK010000000
VERSION     DASTNK010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91762"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1918
                     /locus_tag="VFR20_08905"
     CDS             <1..1918
                     /locus_tag="VFR20_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU0230679.1"
                     /translation="GSPGTVPIAPALDTTMEDARITRGSTTLGHRVLWISLLAVAIGA
                     IAAGIAQLLMFMINVITDVTFYGRVSDVIGPSTIKHTGSFVLSPADNHLGLWVILMPA
                     IGGLIAGIMARWGSRAIQGHGIPEAMEQILQHESNIPPRITWLKPVSSAFAIGTGGPF
                     GAEGPIISTGGALGSLVGQLLSVTATERKTLLAAGAAAGMAAVFGAPVSSLVMAVELL
                     LFELRPRSLIPVALAAVTATGVRYATYGPGAVFAMPAVLEPGGGALASYIVLGAVVGL
                     LSVWVTKAVYGVEDAFEKLPVHWMWWPAIGGLIAGVIGWYAPRTMGVGYDNIDAIVSG
                     SFGVQMLITICVLKFLAWVIALGSGTSGGTLAPLFIIGGSFGALLGIGVDHILPALAI
                     DPRIAALVGMAAIFAGSSRALLTAIVFAFETTRQPAALLPLLGGCTAAYLVSALIMRN
                     TIMTEKIVRRGVLVPLEYSADFLQRMTVGQVYNPDVVTLKAEDTLARILQWMQAGGPA
                     TRYHGYPVVRADGTVLGVITVRDIFNPLTPPETLVSELVTRRPVVVGEDYSLREASDV
                     MVHADVGRLVVMSHDQPARLLGILTRGDLITAHAHRLHESSDARRQLRVNLRRPLRPL
                     RKVRQRKIRTRRAS"
     gene            complement(1947..3695)
                     /gene="ilvB"
                     /locus_tag="VFR20_08910"
     CDS             complement(1947..3695)
                     /gene="ilvB"
                     /locus_tag="VFR20_08910"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEU0230680.1"
                     /translation="MNTLTETQTRNGAQTLLDTLIAHGVDTIFGYPGGAVLPLYDALY
                     SEPRIRHVLVRHEQGAVHAAEGYARSTGKPGVVLVTSGPGMANTTTGLLDALCDSIPV
                     LCISGQVATGAIGTDAFQECDAIGISRPVTKWNAQIRRSEDVASVVGKAYRLTTQGRP
                     GPVLIDFPKDIQIALTPPAAAGAEVSDDAIGIETASPASEAAVKRAASLIANARRPVF
                     YGGGGLINSGLDACHAFTHLVQELGAPCTLTLMGLGAFPADDPLFLGMLGMHGTLEAN
                     LAMHHADLIVCVGARFDDRITGRLSDFCPHASKIHLDIDPASIDKVVRADVAMVGDCY
                     PLLRALHKEVSRINRSPGGLDAWWQRIAVWRKRDCLHVEPSKEHILPQDLMQRVNRLV
                     ADSDAIVSTDVGQHQMWAAQYIRLNRPHRWLTSGGAGTMGYGLPAAIGAQIAHPDKTV
                     ICISGDASVLMNIQELATAMQHKAPVKLILCNNQHMGMVRQWQELIHDGRYSHSYNAS
                     IPDFVALARSFGWGSARVDDPAQLDAALAQCMAHDGPFFLDVAVLAQENCFPMMPAGY
                     GHQQVMLSEDTWYVEE"
     gene            3781..4284
                     /locus_tag="VFR20_08915"
     CDS             3781..4284
                     /locus_tag="VFR20_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEU0230681.1"
                     /translation="MTQTTRPWHIVKPSTLHGNGVYAARKIPAGTRIIEYTGKRITSE
                     QADALHPTNPDDPFHTFFFSTSSGKVIDGGNRGNDARWINHSCAPNCEAEENEAGTRV
                     HIVALRDIARGEELFYDYGLVMDGRITPTLKRQYQCLCGNPSCRGTMLALPKQRTPKA
                     GRKTATA"
     gene            complement(4429..>5088)
                     /locus_tag="VFR20_08920"
     CDS             complement(4429..>5088)
                     /locus_tag="VFR20_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0230682.1"
                     /translation="VAQARESLRTQRAGQLRVEVADVTQEDRIEQLIEDCEQSFGPVA
                     GLVNSAGIAKEIPVLDTDVATFRQILDVNVVGSFIVARAVARRMKTRSRGSIVNIASV
                     SGIRGNYGRVAYGASKGGVITMTQVMAVELARVGIRVNAVAPGPIDTPLTKVVHTAET
                     RKNWVAAVPLQRYGAPEELCGTVGWLLDDAASSYVTGQIISVDGGFTAMGLAFPDAAR
                     D"
BASE COUNT          841 a         1735 c         1647 g          865 t
ORIGIN      
        1 cggcagcccc ggcaccgtac ccatcgcccc ggcgctcgac accaccatgg aagacgcccg
       61 gatcacccgg gggtccacca cgctgggcca ccgggtgctc tggatcagcc tgctggccgt
      121 cgccatcgga gccatcgcgg cgggcatcgc ccagctgctg atgttcatga tcaacgtcat
      181 caccgacgtc actttctacg gacgcgtctc cgacgtcatc ggcccgtcca ccatcaagca
      241 caccggttcg ttcgtcctgt cgcccgccga caatcacctg gggctctggg tcatcctgat
      301 gcccgccata ggcgggctga tcgccggcat catggctcgc tgggggtcgc gcgccatcca
      361 gggccacggc atccccgaag caatggagca gatcctccag cacgaatcga acatcccgcc
      421 gcggatcacc tggctcaagc cggtttcctc ggccttcgcg atcggcacgg gcggcccgtt
      481 cggcgccgaa gggcccatca tctccaccgg cggcgcgctc ggctcgctgg tcggccagct
      541 cctgtccgtc acggccaccg aacgcaagac cctgctggcc gccggcgccg ccgcgggcat
      601 ggcggccgtg ttcggcgccc ccgtgtcctc gctggtcatg gcggtcgagc tcctgctgtt
      661 cgagctgcgc ccgcggtcac tcataccggt cgcgctggcg gccgtcacgg caaccggcgt
      721 gcgctacgcc acctacgggc cgggcgcggt gttcgccatg ccagccgtgc tcgaacccgg
      781 cggcggcgcc ctagccagct acatcgtgtt gggcgccgtc gtgggtttgc tcagcgtgtg
      841 ggtcaccaaa gcggtctacg gcgtggaaga cgccttcgag aaactgcctg tccactggat
      901 gtggtggccc gccatcggcg ggctcatcgc cggcgtcata ggctggtacg caccccgcac
      961 catgggtgtc ggctacgaca acatcgacgc catcgtctcg ggcagtttcg gcgtccagat
     1021 gctgatcacg atctgcgtgc tcaagttcct ggcctgggtc atcgccctgg gcagcggcac
     1081 atccggtggc acgctggcgc cactgttcat catcggtggc tcattcggcg cgctgctggg
     1141 catcggggtc gaccacatcc tgcccgcgct cgccatcgac ccacgcatcg ccgcgctggt
     1201 tggcatggcg gcgatcttcg cgggctcgtc acgcgcgctg ctcacagcca tcgtgttcgc
     1261 gttcgaaacc acgcgccagc ccgcggccct gctgccgctt ctgggcggct gcaccgctgc
     1321 ctatctggtg tccgccctca tcatgcgcaa caccatcatg accgagaaaa tcgtgcgccg
     1381 tggcgtgctg gtcccgctgg aatacagcgc cgacttcctg cagcggatga ccgtggggca
     1441 ggtctacaac cctgacgtcg tgacattgaa ggcggaggac acgctggccc ggatcctgca
     1501 gtggatgcag gcgggaggcc ccgccacccg ctaccacggc tatccggtcg tgcgcgccga
     1561 cggcacggtg ctgggcgtga tcaccgtgcg ggatatcttc aatccactca caccgcccga
     1621 aacccttgtc agcgaattgg tgacacgccg gcccgtggtc gtgggcgagg attattccct
     1681 gcgtgaagca tcggatgtca tggtgcacgc cgacgtgggc cggctggtcg tcatgagcca
     1741 cgaccagccc gcgcggctcc tggggatcct gacccgtggc gacctgatca ccgcgcacgc
     1801 gcatcgactg catgaaagca gcgacgcccg ccggcaactg cgagtgaatt tgcggcggcc
     1861 gttgcggccg ttacggaagg tgcggcagag gaaaatccgg acgcggcggg cgtcttgaaa
     1921 tgatggcgtg ggccgaaacc cgcgcctcac tcctccacat accacgtgtc ttccgacagc
     1981 atgacctgct gatggccata cccggcgggc atcataggaa agcagttctc ctgggccagc
     2041 accgcgacgt ccaggaagaa cggcccgtcg tgcgccatgc attgggccag ggcggcatcc
     2101 agttgtgctg gatcgtcgac gcgggcgctg ccccaaccga acgaccgcgc caacgccacg
     2161 aaatcgggga tggacgcgtt gtagctgtgg ctgtagcgtc cgtcgtggat gagctcctgc
     2221 cactggcgaa ccatgcccat gtgctggttg ttgcacagga tgagcttgac cggcgccttg
     2281 tgctgcatgg cggtggccag ttcctggatg ttcatgagca ccgaggcgtc gccgctgatg
     2341 cagatcacgg tcttgtcggg gtgggcgatc tgcgcgccga tggcggcggg cagcccgtag
     2401 cccatggtgc ccgcgccccc tgaagtcagc cagcggtggg ggcgattcag gcggatgtac
     2461 tgcgcggccc acatctggtg ctgccccacg tcggtggaca cgatggcgtc tgaatccgcc
     2521 accaggcgat tgacgcgctg catgagatcc tgcggcagga tatgttcctt ggagggttcc
     2581 acgtgcaggc agtcgcgttt gcgccacacg gcgatgcgct gccaccaggc atccagccca
     2641 ccgggcgagc ggttgatgcg cgacacttct ttatgcaggg cgcgcagtag cgggtagcag
     2701 tcacccacca tggcgacatc ggcgcgcacc accttgtcga tggaggcggg gtcgatgtcc
     2761 aggtggatct tgctggcgtg tgggcagaag tcggacaacc gcccggtgat gcggtcatcg
     2821 aagcgcgcgc ccacgcagac gatgagatcg gcgtgatgca tggccaggtt ggcctcgagc
     2881 gtgccgtgca tgcccagcat gcccaggaac agcggatcgt cggccggaaa cgcacccagg
     2941 cccatgaggg tgagtgtgca aggcgcgccg agttcctgca ccaggtgcgt gaacgcgtgg
     3001 caggcgtcca gaccggaatt gatcagtccg ccgccgccgt agaacaccgg gcggcgcgcg
     3061 ttggcgatga gcgacgcggc gcgcttgacc gctgcttcgg aagcggggct ggccgtctcg
     3121 atgccgatgg cgtcatctga cacttcagcg ccagccgccg cgggtggcgt cagcgcgatc
     3181 tgaatgtctt tgggaaagtc gatcagcacg gggccaggcc ggccctgggt ggtcaggcgg
     3241 tacgccttgc ccactacgga cgccacgtct tcggagcggc ggatctgcgc gttccatttc
     3301 gtgaccgggc gcgaaatgcc gatggcgtcg cactcctgga aggcatcggt gccgatggcg
     3361 ccggtggcga cctggccgct gatgcacagc accgggatcg aatcacacaa tgcgtcgagc
     3421 aggccggtgg tggtgttggc catgcccggc ccggaggtga ccagcaccac gcccggcttg
     3481 ccggtggagc gcgcatagcc ttcggcggca tgcaccgccc cttgttcgtg ccgcaccagc
     3541 acatggcgga ttcgcggctc ggaatacagc gcgtcgtaca ggggcaggac cgctccaccc
     3601 ggatagccga aaatggtgtc cacaccgtgc gcgatcaagg tgtcaagcag ggtctgggcg
     3661 ccattgcggg tctgggtttc ggtaagcgta ttcacgtgat cagccgtttc gtggattatc
     3721 aggggagcgg tatggcatca taataaactt ttccgttcgc gcctcttcat tcaattcccc
     3781 atgacccaaa ccacacgccc ctggcatatc gtcaaaccct ccaccctgca cggtaacggg
     3841 gtctacgccg cgcgcaagat tccggcgggc acgcgcatca tcgaatacac cggcaagcgc
     3901 atcacgtcgg aacaggccga cgccctgcat cccaccaacc ccgacgatcc gttccatacg
     3961 ttctttttct cgaccagctc gggcaaggtc atcgacggcg gcaaccgtgg caacgacgcg
     4021 cgctggatca atcattcctg cgcaccgaac tgcgaggccg aggaaaacga agccggcacg
     4081 cgcgtgcaca tcgtggccct gcgcgacatc gcccggggcg aagaactgtt ctacgactat
     4141 gggctggtca tggacggccg catcaccccc acgctgaagc gccagtatca gtgcttgtgc
     4201 ggtaatccgt catgccgcgg caccatgctg gctctgccca agcagcgcac gccgaaggcg
     4261 ggccgcaaga ccgcgacggc ctgacgcacg cgcgggacgc atccacaacg ccccgccgga
     4321 gagaccgctt cctctggtga aatgattacg gcaacgggaa tccgttcgcc ctggaccgat
     4381 gtgccgtatc gggcagggcg agtctcgttc aggcgtggcc gcccgggctc agtcccgggc
     4441 agcgtccggg aacgccaacc ccatggcggt gaacccgccg tccaccgaga tgatctgccc
     4501 cgtcacgtag ctggacgcgg catcgtccag cagccagccg acggtgccgc acagttcctc
     4561 tggcgcgcca taccgttgca gcggcaccgc cgccacccag ttcttgcggg tctcggcggt
     4621 atgcacgacc ttggtcaatg gcgtgtcgat ggggcctggc gccaccgcgt tgacgcggat
     4681 acccacccgg gccagctcca ccgccatgac ctgggtcatg gtgatgacgc cacccttcga
     4741 ggcgccatag gccacgcggc cgtaattgcc gcgaatgccc gacaccgacg cgatgttgac
     4801 gatagagccc cgcgaccggg tcttcatgcg ccgtgccacc gcacgggcaa cgatgaacga
     4861 acccaccaca ttcacgtcga ggatctgtcg gaacgtggcg acgtcggtgt ccagcaccgg
     4921 gatttccttg gcgatgcccg ccgagttgac caggccggcc accggtccga acgactgctc
     4981 gcagtcttcg atcaattgct cgatccgatc ctcctgcgtg acgtcggcca cctcgacccg
     5041 caattgaccc gcgcgttgtg tgcgcagcga ttcgcgcgcc tgcgcgac
//
LOCUS       DASTNK010000122         9336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_279637, whole genome shotgun sequence.
ACCESSION   DASTNK010000122 DASTNK010000000
VERSION     DASTNK010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9336
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279637"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VFR20_08925"
     CDS             complement(<1..935)
                     /locus_tag="VFR20_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0230683.1"
                     /translation="MDRFQAMQVFTRVVDTGNFTRAADSLGLPRATVTTAVQNLERHL
                     QVRLLNRTTRHIGLTPDGSAYYERCKLILQDVEEAESTLRDVALQPRGRLRVDTTPTI
                     GRLVIIPALADFYRRYPDMEIALGMGIRPVDLVQEGVDCVIRIGELPDSSLVSRRLGT
                     VHSITCASPEYLDRHGVPQTLDDLGSHQAVLYFSGAGRALDLSFKVDGAIRDIRMPGR
                     ILVNDVEAYIAAALHGHGLIQAPLCLAEPYLASGRLRIVLPQWLPPTLPVSALYLHNR
                     HLSPKVRVFVDWAAELFGDCPGLRLIENGDEILVGR"
     gene            1221..2402
                     /locus_tag="VFR20_08930"
     CDS             1221..2402
                     /locus_tag="VFR20_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0230684.1"
                     /translation="MSTRHNIILTGLAALVVAGLVTYRAGAAPKASKASGGPSAVQVD
                     VATVINRPIIDWHEYSGRLQAVDNVQIRPQVSGTITAVRFKDGALVKKGDVLFIIDPR
                     PYVAAVDRARADVVAAQASVAYTASDERRGQRLLAENAIARRDVEAKRNAAREAAAKL
                     QAAKATLESAELDLEHTKITAPISGRASRANVTVGNVVSASAAPALTSLVSVSHLYAA
                     FDMDERGFLTVSAELRAPGAQAPMVQLRLADEKGYPHKGKLVFIDNQLNPSSGTILAR
                     ALFNNPDGALVPGLYARLRVEEGAQHPAVLIDQTAIGTDQDKRFVLLVDAKNHVQYRQ
                     VRLGGQYDGLQIIDKGLAPGDRIVVNGLQRVRPGMSVAPHIVAMAAPPALAVADIDGN
                     S"
     gene            2439..5642
                     /locus_tag="VFR20_08935"
     CDS             2439..5642
                     /locus_tag="VFR20_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEU0230685.1"
                     /translation="MNISRFFIDRPIFAGVLSALILLAGLLSVFQLPISEYPEVVPPT
                     VVVRAQYPGANPKVIAESVAAPLEESINGVENMLYMQSQANSDGTLTMTISFKLGTDP
                     DKAQQLVQNRVAQALPRLPQEVQRLGVTTAKSSPNLTLVVHLISPSGRYDTTYLRNYA
                     VLNIKDRLSRIPGVGDVLVWGAGDYAMRVWLDPRKVAQRDLTASDVVNAIRQQNVQVA
                     AGTIGAEPSSPSVPMQFSVNAHGRLHTAEQFANIVLKTSPDGAVTRLGDVARVELAAQ
                     QYGLRSLLNNKPAVALGIMQSPGSNALDVSSRVRAAMKSLSADFPAAVQYQIVYDPTQ
                     FVRSSIHAVIETLLEAIALVVLVVILFLQTWRASIIPLLAVPVSIIGTFALLLAFGYT
                     INALSLFGLVLAIGIVVDDAIVVVENVERNIERGLTVREATYQAMREVSGPIVAIALT
                     LVAVFVPMAFMTGLTGQFFKQFAMTIAISTVISAFNSLTLSPALAALLLKPHDAKPDW
                     LTRVLDRVLGRFFRVFNRFFDRSSNNYSSGVTGVIRRKASTFTVYLVLVGAVVALTQI
                     VPGGFVPAQDKQYLISFAQLPNGASLDRTESVIRRMSAIALAQPGVENAVEFPGLSIN
                     GFTNSSSAGIVFITLKPFDQRRSPELSAGAIAGALNRKFAGIKQSFIAVFPPPPVMGL
                     GTVGGFKMQLEDRGALGYAALSKATQAFLAAARKSPELGPSFSSYQIDVPQLDVHVNR
                     VKAMQQGIPLTNIFDTMQIYLGSLYVNDFNRFGRVYQVRVQADAPYRANPEDILQLKT
                     RNRDGQMVPLSALATVTPTFGPEMVTRYNGFTAADINSGPAPGYSSDQAKAAAVRIAD
                     RVLPHGIGFEWTDLAYQQLLAGNAGAWVFPISVLLVFLVLAALYESLTLPLAVILIVP
                     MGVLAALIGVWLTRGDNNIFTQIGLMVLVGLACKNAILIVEFARELELNGMPTIKAAI
                     EASRLRLRPILMTSIAFIMGVVPLVTATGAGAEMRHAMGVAVFFGMLGVTGFGLFLTP
                     VFYVLLRSLSGRRLKTASHHDAPVPTAPHQPQQQESA"
     gene            5645..7222
                     /locus_tag="VFR20_08940"
     CDS             5645..7222
                     /locus_tag="VFR20_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEU0230686.1"
                     /translation="MIKHLKSALSVTALMTLAACSVEPAYHRPTVDVPAAFKEAYLSP
                     AQAHKWMVAHPAEASGRGEWWMVFNDPTLDRLERQALAANQNLKAAAARVKEARAQLG
                     VARSALFPHLDGGFGPLRQRASNASLGLPADASNPPTTLWRAQFGISYEADLFGRVSS
                     ATHAAAADTQQSEALFQSVRLALQADVAQAYFHLRRLDADTDLYRKTLALRSAALDLT
                     QRQYAAGDISDLNVARARTELASTQSEALGIARERAVAEHALAILLGKPPADFSFRPD
                     SLKRTTVTVPAGVPSSLLQRRPDIAAAERAMAAANARIGLAKSAFFPRLDVTGSFGYE
                     SSALGDLLKWSSRTFLLGPLVGTALSLPIFDGGQRGAQLEKARATYDEDVANYRQTVL
                     VAFQQVEDSLANLRLLGGQMKAQDEAVRAAARAATMLHTQYREGSISYLDVIDGDRVL
                     LQQRRASVALDEQRVDSTVALIRALGGGWGPVPNSDDTSATARNTKSESTAGTDTSAG
                     KHPVIPHSPGQSGSVTS"
     gene            complement(7252..8475)
                     /gene="clsB"
                     /locus_tag="VFR20_08945"
     CDS             complement(7252..8475)
                     /gene="clsB"
                     /locus_tag="VFR20_08945"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HEU0230687.1"
                     /translation="MQRKPPRIVWRDGNRITLLRGGSEFFPALCRAIDQAQRSVHIET
                     YLFRLDRTGLRVLEHLRDACSRGVKVRVVLDGYGSAADAGAIVQAIERVGGQCRVYRP
                     EPSGWRSFRLSHRRLRRMHRKLAVIDRREAFVGGINIVDDLDGASDTALSPRFDFAVA
                     VQGPLAFDVAAAQNRLWLRFAWRRPGDWKGFYERLGHWFARVGRDHGKHAPNFTPGVR
                     AALLLRDNLRNRQAIENVYLDAMDQAHDEILVANAYFFPGRRLRHALEQAARRGVRVR
                     LLLQGLAEYPLAFRASRSMYRNMLDEGIEIHEYVAGYLHAKVAVIDGQSMVGSSNMDP
                     FSLLLAREANVFINDSAFTHGLEADLEAALRERARQVTRQSLMRRSVVGRLIDDASYL
                     LLRLGVVLTGRSGEF"
     gene            complement(8493..>9336)
                     /locus_tag="VFR20_08950"
     CDS             complement(8493..>9336)
                     /locus_tag="VFR20_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU0230688.1"
                     /translation="VSYNIHKGRSALGNRESLSDLRLGLYGLRPDLLFLQEVQGRNER
                     HFSLDAQHESLGAALRMNTAYGCNAVRSRTDHGNALLSRYPIVSQENQDISDHPLEQR
                     GLLHVQIRIDDRFVHCLVVHLGLFTGGRSRQVGALVDRIKRLVPANEPLLIAGDFNDW
                     NNRLAPLFVRQLGLFEVFAVAPQVTLASPHRLRNSVRQLGNSLRHLPGPPPRAGAVRE
                     LGHNGAPRLTPPPRTFPAVFPWLRLDRIYQRGFAVRNARVLQGAPWTQLSDHAPLFTE
                     LELP"
BASE COUNT         1704 a         3227 c         2863 g         1542 t
ORIGIN      
        1 cgccccacga ggatctcgtc gccgttctcg atcagccgca accccgggca gtcaccgaac
       61 aactcggccg cccaatcgac gaaaacgcgc accttgggtg aaagatggcg gttgtgtaga
      121 tacaacgccg acacaggcaa cgttggcggc agccactgcg gcagcacgat gcgcaaccgc
      181 cccgacgcca gatacggctc ggccagacac agcggcgcct ggatcagacc gtgcccgtgc
      241 agggcggcgg cgatgtaggc ctcgacgtcg ttgaccagta tgcgccccgg catgcggatg
      301 tcgcgaatgg cgccatcgac cttgaagctg agatccagcg cgcgcccggc gcccgagaaa
      361 tacagcacgg cctggtgact gcccaggtcg tcgagcgtct ggggcacgcc gtggcggtcc
      421 aggtattcgg gcgaggcgca ggtgatgctg tgcacggtgc cgaggcggcg tgaaacaagg
      481 ctggaatcag gcaattcacc gatccggatg acacaatcga ccccctcttg aaccaggtcg
      541 accggccgta ttcccatgcc cagggcgatc tccatgtccg ggtagcggcg gtagaaatcc
      601 gccaaggccg ggatgatgac cagccgcccg atcgtcggcg tggtgtcgac gcgcaggcgc
      661 cccctgggct gaagcgccac gtcgcgcagg gtggattccg cttcctcgac gtcttgaaga
      721 atgagcttgc agcgctcgta atacgccgag ccgtccggcg tcagcccgat gtgccgggtg
      781 gtgcggttga gcaaccgcac ctgaagatga cgttccagat tctggaccgc cgtggtgacc
      841 gtggcccggg gcaggccgag gctgtctgcc gcccgggtga agtttcccgt gtcgacgacc
      901 cgggtgaaga cctgcattgc ttgaaaacga tccacgttac cgctccgtta gggctcgttc
      961 gccctgaatc acgcccctcg tggaagcgtg ccgggggaac aggcaggccg cgcgcgccct
     1021 gcgccgaccc ggacgaagca ctctggacag gccggccgga cccccggtgg gattgtttgg
     1081 attttctgaa aagaattcgc aaatactagc tatttatcca ggaaacttca acgcgcaaaa
     1141 tatgacgttt cttttctgta ctaattcagt accaattgat cgggcacggc tcgaagccgg
     1201 agcccttcag gggaaacaag atgtccacgc gtcacaatat cattttgacc ggcctggcgg
     1261 cactggtcgt tgccggcctg gtcacctacc gcgccggggc cgcccccaag gcgtccaagg
     1321 cgtccggggg cccatcggcc gtgcaggtcg acgtcgccac cgtcatcaac cgccccatca
     1381 tagactggca cgagtattcg ggtcgattgc aggccgtcga caacgtccag atccgccccc
     1441 aggtatccgg caccatcacc gcggtccggt tcaaggacgg cgcgctggtc aagaagggag
     1501 acgtgctgtt catcatcgac ccccgcccct acgtcgcggc agtggacagg gcacgcgccg
     1561 acgtggtcgc cgcccaggcc agcgtcgcct acacggcctc tgacgaacgg cgcggccagc
     1621 ggctgctcgc cgaaaacgcc atcgcccgcc gcgacgtcga agccaagcgc aacgcggcac
     1681 gcgaagcggc cgcgaaactg caggcggcca aggcaaccct ggaatccgcc gagctcgatc
     1741 tggagcacac gaagatcacc gcgccgatct cgggccgcgc ctcgcgcgcc aatgtcacag
     1801 tgggaaacgt ggtgtcggcc agcgccgcgc ccgcgctcac ctcgctggtc tcggtatcgc
     1861 acctgtacgc cgcgttcgat atggatgaac gcggtttcct gaccgtatcg gccgagttac
     1921 gcgcccctgg cgcccaagcc ccgatggtgc aactgcggct ggccgacgaa aaaggctacc
     1981 cccacaaggg caagctggtg ttcatcgaca accagctcaa cccctcgtcg ggcaccatac
     2041 tggcccgcgc cctgttcaac aacccggacg gcgcgctggt ccccggacta tacgcccgcc
     2101 tgcgcgtcga ggaaggggcg cagcacccgg ccgttctcat cgatcaaacg gccatcggca
     2161 ccgaccagga caaacgattc gttctgctgg tcgacgccaa gaaccacgtg cagtaccgac
     2221 aggtgcggct gggcggccag tacgacggct tgcagatcat cgacaaaggc ctggctcccg
     2281 gcgaccgcat cgtcgtcaat ggcctgcagc gcgtgcgccc tggcatgagc gtcgcccccc
     2341 acattgttgc catggccgca ccccccgcgc tggccgtggc cgacatcgat ggcaactcct
     2401 gacggaacgc catcccacac ggtttctaag cgattgtcat gaacatatcc cgatttttca
     2461 tcgaccggcc cattttcgcc ggcgtgctgt ccgccctgat cctgctcgcc ggcctgctgt
     2521 cggtgtttca gctgcccatt tccgaatacc ccgaggtcgt gccgcccacg gtggtggtgc
     2581 gcgcccagta ccccggcgcc aatcccaagg tcatcgccga gtccgtggcc gcgccactcg
     2641 aggaatccat caacggcgtc gagaacatgc tgtacatgca gtcgcaggcc aacagcgacg
     2701 gcacgctcac catgaccatc agcttcaagc tgggcacgga ccccgacaag gctcagcaac
     2761 tggtgcagaa ccgcgtcgcg caggcactgc cgagattgcc gcaggaagtc cagcggctgg
     2821 gcgtgaccac ggccaagagt tcgcccaacc tcactctggt ggtgcacttg atctcgccca
     2881 gcgggcgcta cgacaccacc tatctgcgca actacgcggt gctcaacatc aaggatcgcc
     2941 tgtcgcgcat ccccggggtg ggcgacgttc tggtctgggg ggcgggcgac tacgccatgc
     3001 gcgtctggct cgatccgcgc aaggtcgcgc agcgcgacct cactgcctcg gatgtggtca
     3061 acgccatccg ccagcagaac gtccaggtgg ccgccggcac cattggcgcg gagccaagct
     3121 cgcccagcgt gcccatgcag ttctcggtga acgcgcacgg ccgcctgcac acagcggaac
     3181 agttcgctaa tatcgtgctg aagacatctc ccgacggcgc ggtgacccgc ctgggtgacg
     3241 tggcgcgcgt cgaactcgcc gcccagcaat acggcctgcg ctcgctgctg aacaacaagc
     3301 ccgctgtcgc gctgggcatc atgcagtcgc cgggctccaa cgccctggat gtctcgagcc
     3361 gtgtccgggc cgccatgaaa tcgctctcgg ccgacttccc cgccgcggtg caataccaga
     3421 tcgtgtacga ccccactcag ttcgtgcgct ccagcattca tgccgtcatc gagacgctgc
     3481 tcgaagccat cgccttggtg gtgttggtgg tgatcctgtt cctgcagacc tggcgcgcgt
     3541 ccatcattcc cctcctggcc gtgccggtgt ccatcatcgg cactttcgcc ctgctgctgg
     3601 ccttcggcta caccatcaac gcgctgtcgt tgttcgggtt ggtgctggcc ataggcatcg
     3661 tggtcgatga cgccatcgtg gtggtcgaga acgtggagcg caatatcgag cgcgggctca
     3721 cggtgcggga ggccacgtat caggccatgc gcgaagtcag cgggcccatc gtcgccatcg
     3781 ccctgacact tgtcgcggtg ttcgtgccca tggcgttcat gacgggcctc accggccagt
     3841 tcttcaaaca attcgccatg accatcgcga tctcgaccgt gatctcggcg ttcaactcgc
     3901 tgaccctgtc gcccgccctg gcggcgctgc tgctcaagcc gcacgacgcc aagcccgact
     3961 ggctcacgcg cgtgctcgac cgggtcctgg gccggttctt cagagtattc aaccgcttct
     4021 tcgatcgatc ctcgaacaac tactccagcg gcgtcaccgg tgtcattcgg cgcaaagcct
     4081 ccacgttcac ggtgtacctg gtgctggtgg gcgccgtggt cgcgctgacc cagatcgtgc
     4141 caggcggctt cgtgcccgcc caggacaagc aatacttgat cagcttcgcg caattgccaa
     4201 acggcgcgtc gctcgaccgc accgaaagcg tgatccgccg catgagcgcc atcgcgctgg
     4261 ctcagccggg agtcgagaac gcggtcgaat tccccggcct atccatcaac ggtttcacca
     4321 acagttccag cgccggtatc gtgttcatca cgctcaaacc gttcgaccag cgccgttctc
     4381 cggagctgtc ggcgggtgcc atcgccggag cgctgaaccg caagttcgcg ggcatcaagc
     4441 aatccttcat cgcggtgttc cccccacccc ccgtcatggg cctgggcact gtgggcgggt
     4501 tcaagatgca gctcgaggat cgcggcgcgc tgggctacgc ggcgctgagc aaggccaccc
     4561 aggcattcct cgccgccgcg cgcaaatcac ccgaactcgg gccatcgttc tccagctacc
     4621 agatcgacgt gccccagctc gacgtgcatg tgaaccgcgt caaggccatg cagcagggca
     4681 tcccgttgac caacatcttc gacaccatgc agatctacct ggggtcgctc tacgtcaacg
     4741 acttcaaccg ctttggccgc gtgtaccaag tgcgggtcca ggctgacgcg ccttatcgcg
     4801 ccaacccgga ggacatcctg caattgaaga cccgcaaccg cgacgggcaa atggtgccgc
     4861 tgtcggcgct ggccaccgtc acacccacgt tcggacccga gatggtcacg cgctacaacg
     4921 ggttcaccgc cgccgacatc aacagcgggc cggcaccggg ctattcatcg gaccaggcca
     4981 aggccgccgc cgtgcgtatc gcagatcgcg tgctacccca cggcatcggc ttcgagtgga
     5041 ccgacctggc ctatcagcag ctcctggcgg gcaacgccgg cgcctgggtc ttccccatca
     5101 gcgtcctgct ggtgttcctg gtgctggccg cgctctatga aagcctgaca ctgcccctgg
     5161 ccgtgattct catcgtgccc atgggcgtgc tggcggcgct gatcggcgtg tggctcacac
     5221 gcggcgacaa caacatcttc acccagatcg ggctcatggt gttggtgggg ctggcctgca
     5281 agaacgccat cctgatcgtc gaattcgcgc gtgaactgga gctcaacggc atgcccacga
     5341 tcaaagccgc catcgaggcc agccgcctgc ggctgcgccc catcctcatg acttccatcg
     5401 cgttcatcat gggtgtcgtg ccactggtca cagccaccgg cgcaggcgcc gaaatgcgtc
     5461 acgccatggg cgtggcggtg ttcttcggca tgctgggggt caccggattc ggtctgttcc
     5521 tgaccccggt gttctatgtc ctgctgcgct cgctcagcgg gcgccggctg aaaaccgcct
     5581 cgcatcacga cgccccggtt ccgaccgccc cgcatcaacc gcaacaacaa gaatccgcat
     5641 gatcatgatc aagcatctca aatccgccct gagcgtgacg gcgctgatga ctttggcggc
     5701 gtgctcggtc gagcccgcct accaccgccc cacggtcgac gtgccggcgg cgttcaaaga
     5761 ggcctacctg tcgccggccc aggcgcacaa atggatggtc gcccatcccg ccgaggccag
     5821 cggccgaggc gaatggtgga tggttttcaa tgacccgact cttgaccgcc tggagcgcca
     5881 ggcgctggcg gccaaccaga acctcaaggc ggccgcggcc cgggtcaagg aagcccgggc
     5941 acaactgggt gtggcccgct cggcattgtt cccccatctg gacggtggct tcggccccct
     6001 gcgccaacgc gcctcgaacg cctccctggg tctgcccgcc gacgcgtcga acccgcccac
     6061 cacgttgtgg cgggctcagt tcggcatttc gtacgaggcc gacctgttcg gccgcgtgtc
     6121 ctccgccaca cacgccgcgg cggccgacac ccaacagagc gaggccttgt tccaatccgt
     6181 gcggctggcc ctgcaggcag acgtcgcgca ggcatacttt catctgcgcc gactcgatgc
     6241 cgacacggac ctgtaccgca agacgctggc cctgcgctcc gcggcgctcg acctcactca
     6301 acgtcagtac gcagccggcg acatcagcga cctgaacgtg gcgcgcgcca gaaccgagct
     6361 ggcctccacc caatcagagg cactcggcat cgcccgcgag cgcgccgtgg cggaacacgc
     6421 cctggccatc ctcctgggca agccgccggc cgatttctcg ttcagacccg attccctgaa
     6481 gcgcaccacc gtgacagtcc ccgcgggggt cccatcgtca ttgctgcagc ggcgccccga
     6541 tatcgccgcc gcggaacgcg ccatggcggc ggcgaacgcg cgcatcggac tagccaaaag
     6601 cgctttcttc ccgcgactgg acgtcaccgg ctctttcggc tatgaatcct cggcattggg
     6661 cgacctgctg aaatggagca gtcgcacgtt cctgctgggg cccctggttg gcacggcgct
     6721 cagcctgccc atcttcgacg gtggccagcg cggcgcccag ctggaaaagg cccgtgccac
     6781 ctatgacgaa gacgtcgcca actatagaca gactgtgttg gtcgccttcc agcaggtgga
     6841 ggacagcctg gccaacctgc gcctgctggg tggccagatg aaggcccagg acgaggcggt
     6901 gcgggcggcc gcccgtgcgg cgacgatgct gcacacgcag tatcgcgaag gctcgatcag
     6961 ctatctcgat gtcatagatg gcgacagggt gttgctgcag cagcggcgcg cctcggtcgc
     7021 gcttgatgag caaagggtcg attccacggt cgcgctgatc cgcgcgctgg gtggaggctg
     7081 ggggcccgtt ccgaatagtg acgacacgtc cgccaccgcc cgcaacacca agagtgagag
     7141 cacagccggc acggacacat cggcgggcaa gcacccagtg atcccccata gccccggaca
     7201 gtcaggctcg gtcacgtcgt gacggcccct gcttctgata ctgaaggccg gtcagaattc
     7261 acctgaccgg ccggtcagca caacgcccag gcgcagcagc aaatacgacg catcgtcgat
     7321 caggcggccc accacggaac gcctcatcag actctgccgc gtcacttggc gggcacgctc
     7381 acgcaatgcc gcctccagat cagcctcgag cccatgggtg aaggcggaat cattgatgaa
     7441 cacgttggcc tcgcgcgcca gcaacagact gaaaggatcc atgttcgacg aaccaaccat
     7501 ggactgacca tcaatcacgg ccaccttggc atgcaggtaa cccgccacgt actcgtgaat
     7561 ctctatgccc tcgtcgagca tattccggta catcgagcgg ctggcccgga acgccagcgg
     7621 atattccgcc aacccctgca gcagcagccg cacccgcacc ccgcgccggg cggcctgctc
     7681 cagcgcgtga cgcaggcgac ggcccggaaa gaaataggca ttggccacca ggatctcatc
     7741 gtgtgcctgg tccatggcat cgagatacac attctcgatg gcctggcggt tgcgcaggtt
     7801 gtcacgcagc agcaaggcgg ctctgacgcc aggcgtgaag ttgggcgcat gcttcccgtg
     7861 gtcccgaccc accctggcga accaatgccc cagccgttca tagaagccct tccaatcgcc
     7921 tggccgacgc catgcgaagc gcaaccacag gcgattctgc gcggccgcca cgtcaaaggc
     7981 cagcggacct tgcaccgcca cggcgaaatc aaaccgtggg ctgagcgccg tatcggacgc
     8041 accgtccaga tcatccacga tgttgatgcc gcccacgaag gcttcccgcc ggtcgatcac
     8101 ggccagcttg cgatgcatgc gacgcagtct gcggtgactg agcctgaaac tgcgccatcc
     8161 cgaaggttcg ggccgataga cgcggcactg gccccccacg cgctcgatcg cctggacgat
     8221 ggccccggcg tcggcggcgc tgccatagcc atcgagaacg acccgcacct tcacgccgcg
     8281 cgaacaagcg tcgcgcagat gttcgaggac tcgcaacccc gtgcggtcca ggcggaagag
     8341 ataggtctcg atatgcacgg agcgctgtgc ttgatcgatg gcgcggcaca gggcgggaaa
     8401 aaattccgag ccgccacgca acaaggtgat gcggtttccg tcacgccaga cgatgcgggg
     8461 tggcttgcgt tgcacagcga taacgaggcc ccctacggca attcaagctc ggtgaacaac
     8521 ggcgcgtgat cggacaactg cgtccagggc gccccttgaa ggacccgggc gttgcgcacc
     8581 gcgaacccac gctgataaat gcggtccagc cgcagccacg gaaacacggc gggaaacgtg
     8641 cgaggtggcg gcgtgaggcg tggcgcccca ttgtggccga gttcacgcac cgcgcccgcc
     8701 cggggcggcg gacctggcag gtgccgcaat gaattgccga gctgccggac cgaattgcgc
     8761 aagcggtgtg gactcgccag agtcacttgt ggcgccacgg cgaacacctc gaacaggcca
     8821 agctgccgca cgaacaacgg cgccaggcgg ttgttccaat cgttgaaatc gcctgcgatg
     8881 agcagcggct cgttggccgg gaccaggcgc ttgatgcgat cgaccagcgc gccgacctgg
     8941 cgtgaacggc cacccgtgaa caaccccaga tgcaccacca gacaatgcac gaaccgatca
     9001 tcgatgcgga tttgcacatg cagcagtccg cgctgctcga gcgggtgatc ggaaatatcc
     9061 tggttttcct gactgacgat ggggtagcgc gacagcagcg cgttgccatg atcggtacgg
     9121 ctgcgcaccg cgttgcagcc ataggcggtg ttcatgcgca gggcggcgcc cagcgattcg
     9181 tgctgcgcgt ccaggctgaa atggcgttcg ttgcggcctt gcacttcctg caggaacagc
     9241 aggtctggac gcagaccgta caggcccagg cgcagatcgg acagggactc ccgattgccc
     9301 agcgccgaac ggcccttgtg aatgttgtag ctgacg
//
LOCUS       DASTNK010000123         7581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_836604, whole genome shotgun sequence.
ACCESSION   DASTNK010000123 DASTNK010000000
VERSION     DASTNK010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7581
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836604"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..904
                     /locus_tag="VFR20_08955"
     CDS             53..904
                     /locus_tag="VFR20_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEU0230689.1"
                     /translation="MLYPELFKSMEAVRWNMASDIPWDDFDASKLSDEQAYTIKMNAI
                     TEWAALPATEMFLRDNRDDSDFSAFMSIWFFEEQKHSLALIEYLRRFRPELMPTEEEL
                     HNVRFEFDPAPAMETLMLHFCGEIRLNHWYRRASEWHTEPVIKAIYKIISQDEARHAG
                     AYLRYMKRALVERGKSFGQQARIAFSKIGVLMASAQRTSQALHPTNLHVNKDMFPADT
                     VQSKLPSPGWLENWLDHQICFDKVWENKVSARILHNMSLLMGSTFETVQDLNRYRKGL
                     IKSPTPA"
     gene            914..1420
                     /gene="rfaE2"
                     /locus_tag="VFR20_08960"
     CDS             914..1420
                     /gene="rfaE2"
                     /locus_tag="VFR20_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEU0230690.1"
                     /translation="MPARFESKILTPHACINAARVGGLPRPLVFTNGVFDILHRGHVT
                     YLDAAAQRGATLIVALNTDASVRRLGKGSDRPVNGLEDRAALLAALGCVDVVTSFDED
                     TPLALIEALRPDIIVKGGDYDMETLPETAAVRRWGGQALAIPFEFQRSTTTLLGKIRG
                     DAARDLKT"
     gene            complement(1410..2090)
                     /locus_tag="VFR20_08965"
     CDS             complement(1410..2090)
                     /locus_tag="VFR20_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEU0230691.1"
                     /translation="MNATALSELEQARFNMIEQQVRPWGIFDERVLQALFSTRRERFV
                     PTSVQGLAFSDIELPLIINAVDTRETMLAPKVEARLAQELQLAPTDCVLEIGTGSGYQ
                     AALLAQLAQQVTSIEVSSRLANFAQQNLQRNQVTNVKVETGDAHTGWGTTEYDAILLT
                     GSVPTVPDALKYQLRIGGRMVVVVGQNPIMSACRITRTSAASFETVSLFDTVIKPLRG
                     VVVSHFKF"
     gene            complement(2224..3036)
                     /gene="thiD"
                     /locus_tag="VFR20_08970"
     CDS             complement(2224..3036)
                     /gene="thiD"
                     /locus_tag="VFR20_08970"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU0230692.1"
                     /translation="MIPNTLTIAGVDPSGGAGILADIKTMSALGAYGTAVVAALTAQN
                     TRAVTSISPVPPAFVTAQIDTLFSDVRIDAVKVGMLGQQPVIRCVAESLSRWKPPHLV
                     IDPVMVAKSGDHLLERDAIGELRGHLLPLATVMTPNLPEAGVLLNATPAESVKEMRRM
                     AEKLRRLLGDDGERWVFLKGGHLPGSSTVDILHDGDRMLELPGERIDTPNTHGTGCTL
                     SAAVAALLPQVGDVPEAARRAKRFLEEAIRQSGALAVGTGHGPVHHFHAWWR"
     gene            complement(3254..4222)
                     /locus_tag="VFR20_08975"
     CDS             complement(3254..4222)
                     /locus_tag="VFR20_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEU0230693.1"
                     /translation="MTRSARGWARQTTHAGRRREKRAALTIFLVLALTTWTGTTQAAP
                     GLSPADAGLGPSPSGPIVRPAPHTFPRRIITLAPHITELVFAAGAGDRIVGTVSSSDY
                     PPQARHIPRVGNGIELDTERIVALRPDLILAWQPSGATRALAPLLSRLHIPLAYVQPH
                     ALRDIPAEVAGLGRRLGTTAIADPEAHELTERIAALAAHYEHQRVVSTFIEVGAAPLY
                     TLGKAPLINDALRVCGGSNIFAGAAIPAPQVSIESVLRAQPDAIIIPSDDTRRVAQRT
                     HYWADLSLRAALDRHVYGIDPDELFRPGPRVIDATQTLCQDLAKAR"
     gene            complement(4222..6132)
                     /locus_tag="VFR20_08980"
     CDS             complement(4222..6132)
                     /locus_tag="VFR20_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0230694.1"
                     /translation="MKAITSRHALAIACALAPCLATAQTSSNATPPSSTSTTPTPAIT
                     ELNDIVVTGRRSPQPERDVLGDATIIDHQTLENAGQSSLAQVLSQEHGIEFYDNGGPQ
                     TATGISIRGANTDQTLVLLDGVPINGATSGLAALNALPPDSIDHIEILRGSASSLYGA
                     DAIGGVINVITRKGSDKPFSAQASAGVGTYGTSKYSAGFSGSAGGWYYSLGASYGQSS
                     GYNATNSHNTYIYNPDKDSYYSSNVNGSLGYEWKKGQKITFQAYNTRINGGIDYDRFN
                     PFNDRSIQTLEMYSLASENQITDFWKSTLRYAYTLDKNRTISATSDTTFSTRQDQYSW
                     QNDLTLSKTQKLTLAYEYLAQQASGQIPSYNPDFTPGPMLNFNKTRRHNNAFTGVYSG
                     DFGRNHVQASLRNDDDSQFGPATTWGLSYGYDLTPHLRGYVAANTGFKTPTFNDLYYP
                     GFANPNLQPEKSRNVEAGLKYTGDTYLLGIVVYRNKVRNLITFDPAVNKPENLGQATL
                     RGVTLTGEKDFGNTTLRASADFQDPHNDTDDKQLNRRARQIYRLSADHRMGPWTLGGE
                     YQFVGHRYDDVQNTVDLGGYGLVNLTAGYRIDKNLEVSVRWNNVLGRDYTTAYGYNTP
                     GSNVFVNLRWMM"
     regulatory      complement(6208..6411)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6658..>7581
                     /locus_tag="VFR20_08985"
     CDS             6658..>7581
                     /locus_tag="VFR20_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEU0230695.1"
                     /translation="MSYPVSAYPIESYTRGAEFARLLGSRILILDGAMGTMIQRYKLT
                     EADFRGERFANHSKDVKGDNELLSLVRPDVILEIHRQYLEAGSDVIETNTFGATAVAQ
                     GDYGLADIAYDLNLASAKLARQACDEFSTPDKPRFVAGALGPQPKTASISPDVNDPAA
                     RNVTFEELRVAYAEQLNGLLDGGIDLVLIETIFDTLNAKAAIFAVESVFEERGVRLPV
                     MISGTVTDASGRILSGQTVEAFWNSVRHARPITIGLNCALGAALMRPYIAELSKICDT
                     YICVYPNAGLPNPMAETGFDETPCDTSSLLEE"
BASE COUNT         1300 a         2271 c         2510 g         1500 t
ORIGIN      
        1 tgtgactcga gcggagagaa acccgcctgc gccgaacttc aagagggatc ctatgcttta
       61 tccggaattg ttcaaatcga tggaggcggt acgctggaac atggcatccg acatcccatg
      121 ggatgatttc gatgcctcca aactgtccga cgagcaggcg tacacgatca agatgaacgc
      181 catcaccgaa tgggccgctc tgccggccac cgaaatgttc ctgcgcgaca atcgcgacga
      241 cagtgatttt tcggccttca tgtccatctg gttcttcgag gaacagaagc actccctggc
      301 cctcattgag tacttgcggc gttttcgccc cgaactcatg ccgaccgagg aagagctcca
      361 caacgtgcgg ttcgaattcg atccggcccc cgcgatggag acgctgatgc tgcatttctg
      421 tggcgagatc cgtttgaatc attggtatcg ccgggcgtcg gagtggcaca ccgagccggt
      481 catcaaggcg atctacaaga tcatctccca agacgaggcc cgccacgccg gcgcatacct
      541 gcgctacatg aagcgcgcgc tggttgaacg cggcaagtcg tttggccagc aggcgcgcat
      601 cgcattctcg aagatcggcg ttctgatggc ttcggcgcaa cgaacgtcgc aggcgttgca
      661 tcccacgaat ctgcacgtca acaaagacat gttccctgcc gatacggtgc aatcgaaact
      721 gccgtcaccg gggtggctcg agaattggct cgatcaccag atatgcttcg acaaggtctg
      781 ggagaacaaa gtcagtgcgc gcatcctgca caatatgtcg ctgctcatgg gcagcacttt
      841 cgagaccgtc caggacttga atcgttatcg caaaggcctc atcaaaagcc cgacgccagc
      901 ctgagccttg accatgccgg ctcgcttcga atccaagatc ctgacaccgc atgcctgcat
      961 caacgcggcg cgcgtcggcg ggttgccccg ccctctggtg ttcacgaatg gggtgttcga
     1021 cattctgcac cgtggccatg tcacgtacct cgatgccgcc gcgcaacggg gggccaccct
     1081 gattgtcgcg ttgaataccg atgcatcggt gcgacgcctg gggaaaggat ccgaccggcc
     1141 cgtcaatggg ctggaagacc gggccgcgtt gctggcggcc ctggggtgcg tggacgtggt
     1201 caccagtttt gacgaagaca ctccgctggc gctgatcgag gcgctgcggc ccgacatcat
     1261 cgtcaaaggg ggggattacg acatggaaac cctgcccgaa accgccgcgg taaggcgttg
     1321 gggtggccag gcattggcca ttccgttcga gttccagcgc tcgaccacga cgctgctggg
     1381 aaagatccgc ggcgatgccg cgcgcgacct caaaacttga agtgcgagac caccacgccg
     1441 cgcagcggct tgatgaccgt gtcgaacaag ctgacggttt cgaagctggc cgcgctggtg
     1501 cgggtgatac ggcaggctga catgatgggg ttctggccca ccacgacgac catgcggccg
     1561 ccaatgcgca actggtattt cagcgcgtcg ggcaccgtgg gcaccgatcc ggtgagcagg
     1621 atggcgtcgt attccgtcgt gccccagccg gtatgcgcgt cgccggtttc caccttgacg
     1681 ttggtgacct gattacgctg caggttttgt tgagcgaagt tggccagccg gctgctgacc
     1741 tcgatgctgg tgacctgctg tgcgagttgt gccagcaggg cggcctggta gccggatccc
     1801 gtgccgattt cgagcacaca gtcggtgggc gccagttgca gttcctgggc aagccgggcc
     1861 tcgaccttgg gtgccaacat ggtttcgcgc gtatccaccg cgttgatgat caggggcagt
     1921 tcgatgtcgg agaatgccag gccctgcacg gaggtgggga cgaagcgttc gcgccgcgtg
     1981 gagaacaggg cctgcaggac acgttcgtcg aaaatgcccc acggacggac ctgctgctcg
     2041 atcatgttga aacgagcctg ttcgagttct gatagcgctg tagcgttcat ggcctgtatc
     2101 gtgaaaaacg ttgacggtgg acgattgtat tgtactgatt tcaccgcgtg gcgcggccgg
     2161 cgccacgcag gatgccgccg gccccttggt ggaaagacat ttggcggacg tggcgcgccc
     2221 gtctcagcgc caccaggcgt ggaagtgatg cacggggcca tggcccgtgc cgacggccag
     2281 ggcgcccgat tgccgaatgg cttcttccag gaagcgtttg gcgcgtctgg ccgcctcggg
     2341 cacgtcgcca acctgtggca gtagcgcggc cacggcggcc gacaaggtgc aacccgtgcc
     2401 gtgcgtgttg ggcgtgtcga tgcgttcccc gggcaattcc agcatccggt caccgtcgtg
     2461 caggatgtcg accgtgctcg aaccgggcag atgcccgcct ttcaggaaca cccagcgttc
     2521 accgtcgtcg ccgagcagcc ggcgcagttt ttccgccatt cggcgcattt ctttcaccga
     2581 ttcggcgggc gtggcgttca acaacacgcc ggcttcggga agattgggcg tcatcacggt
     2641 cgcgagaggc agaaggtgcc cgcgcagttc gccaatggcg tcacgctcca gcagatggtc
     2701 cccgctcttg gccaccatga ccggatcgat gaccaggtgg gggggcttcc atcgggagag
     2761 gctttccgcc acgcatcgta tgaccggttg ctggcccagc atgccgacct tgacggcgtc
     2821 gatgcgcaca tcgctgaaca aggtgtcgat ctgggcagtg acgaaggccg gtggcacggg
     2881 actgatactg gtgacggcgc gcgtgttctg ggcggtcagg gccgcgacga ccgccgtgcc
     2941 gtaggcgccc agggcgctca tggttttgat gtcggccagt atgccggcac cgccggaggg
     3001 gtcgacgcca gcgatggtaa gggtattggg aatcatgcga aggctttcct gatggccagg
     3061 ggctgcgcgc gagcggggcc cggcgatacg gtatccaatg gccggcgcga tccggagcgt
     3121 ggtcgcgcgg cgtcaaggat aatcgatttg ccgatgcctt gtctggttgg ctgtttcaag
     3181 gggcgtcgcg atgacaacgg ccatcaccgc ggcttgtgcc gcgcgtccga cttggttggc
     3241 gcgcagtacc tggttatcgg gccttggcca ggtcctggca cagcgtctgg gtggcgtcga
     3301 tcacgcgcgg gcctggccgg aacaattcgt cgggatcgat gccgtagacg tgccgatcca
     3361 gcgcggcgcg cagagacaga tcggcccagt aatgggtccg ttgcgccacg cgtcgcgtat
     3421 cgtcggacgg aatgatgatg gcgtcgggct gggcccgcaa cacgctttca atgctgacct
     3481 gcggcgcggg gatggccgca ccggcgaaga tattgctgcc gccgcagacc cgcagggcgt
     3541 cgttgatcag gggggccttg cccagcgtgt agagcggagc ggcgccgacc tcgatgaatg
     3601 tcgataccac tcgctgatgc tcgtaatggg cagccagcgc cgcgatgcgc tccgtcagct
     3661 cgtgggcttc cgggtcggcg atcgcggtcg tgcccagcct gcgccccaga cctgccacct
     3721 ccgcggggat gtcgcgcagc gcgtgtggct gcacgtaggc caggggaata tgcaggcggg
     3781 acagcagggg cgcgagtgcg cgtgtggcgc cggacggctg ccaggccagt atcagatcgg
     3841 gacgcagggc gacgatgcgt tccgtatcga gctcgatacc gttgcccaca cgcgggatgt
     3901 gtcgtgcttg tggagggtag tcgctgctgg acaccgtgcc cacgatgcgg tcaccggccc
     3961 ccgcggcgaa caccagttcg gtgatgtgcg gcgccaacgt gatgattcgg cgcggaaacg
     4021 tatgcggcgc tggacgaacg attggtccag aaggagaggg gcccaaaccc gcatccgctg
     4081 gcgaaagccc cggcgcggcc tgggtggttc cagtccaggt ggtcagcgca agcaccagga
     4141 agatggtcag cgccgcacgt ttctcgcgtc ggcgccccgc gtgggtcgtc tggcgggccc
     4201 agccgcgtgc cgaacgcgtc atcacatcat ccatctcagg ttcacgaata cgttcgaccc
     4261 cggcgtgttg tagccgtatg ccgtcgtgta gtccctgccc agcacgttgt tccagcgcac
     4321 gctgacctcc aggttcttgt cgatgcgata gcctgcggtc aggttcacca ggccgtagcc
     4381 acccaggtcc accgtgttct gtacgtcatc atagcggtgt cccacgaact ggtattcacc
     4441 acccagggtc cacggcccca tgcgatggtc ggcgctgagg cgatagattt gccgggcacg
     4501 acggttcagc tgcttgtcgt cggtgtcgtt gtggggatcc tggaaatccg cgctggcgcg
     4561 cagcgtggtg ttgccgaaat ccttttcccc ggtcagtgtc acaccgcgca aagtggcctg
     4621 gcccagattt tcgggcttgt tcacggccgg atcgaacgtg atcagattgc ggaccttgtt
     4681 gcgatagacg acgatgccca gcagataggt gtccccggtg tatttcaggc ccgcctcgac
     4741 gttgcgcgat ttttccggtt gcaggttcgg attggcgaaa ccggggtagt acagatcgtt
     4801 gaaggtcggc gtcttgaagc cggtgttggc ggccacatag ccgcgcaggt gcggcgtgag
     4861 atcgtagccg taactcagcc cccaggtcgt ggccgggccg aactgcgaat cgtcatcgtt
     4921 gcgcaggctg gcctgcacgt ggttgcggcc gaagtctccg gaataaacgc cggtgaacgc
     4981 gttgttgtgc cgccgcgtct tgttgaaatt cagcatcggc cccggcgtga agtcagggtt
     5041 gtaagacggg atctgaccag atgcctgctg cgccaggtac tcgtaggcca gggtcagttt
     5101 ctgcgtcttg ctcaacgtca ggtcgttctg ccaggagtac tggtcctggc gtgtgctgaa
     5161 cgtggtgtcg ctggtggctg atatcgtgcg gttcttgtcg agcgtgtagg catagcgcag
     5221 cgtgcttttc cagaaatcgg tgatttggtt ctcgctggcc aggctgtaca tttccagcgt
     5281 ctggatgctg cggtcgttga acgggttgaa ccggtcataa tcgatgccgc cattgatgcg
     5341 cgtgttgtag gcctggaagg tgatcttctg gcctttcttc cattcgtagc ccagcgagcc
     5401 gttcacattg ctcgagtaat agctgtcctt gtccgggttg taaatatacg tgttgtggct
     5461 attggtggcg ttgtagccac tgctctggcc gtatgatgcg cccaggctgt agtaccaccc
     5521 ccccgcgctg cccgaaaagc ctgcggagta tttgctggtg ccataggtgc cgactccggc
     5581 cgacgcctgg gctgagaacg gtttgtccga gccttttcgg gtgatcacgt tgatcacgcc
     5641 gccaatggcg tcggcgccat acaagctgct ggccgaaccg cgcaggattt cgatgtgatc
     5701 gatggagtcg ggcggcagcg cgttgagcgc ggccaagccg ctggtcgcgc cgttgatcgg
     5761 cactccgtca agcaggacca gcgtttgatc ggtattggcg ccccgaatgg atatgccggt
     5821 ggccgtctgc ggcccgccat tgtcgtagaa ctcgatgcca tgctcctgcg acaacacttg
     5881 cgccagactg ctctgcccgg cgttctccag ggtttggtgg tcgatgatgg tggcatcgcc
     5941 caggacgtcg cgttcgggtt gcggcgagcg gcgccccgtg acgacgatgt cgttcagttc
     6001 tgtgatggcc ggggtgggtg tcgtggaagt cgaggagggg ggcgtcgcgt tggatgaggt
     6061 ttgcgcggtg gctagacatg gcgcaagcgc acaggcgatg gcgagcgcgt gccgggaggt
     6121 gatggctttc atggaatgac ctggaaataa tgtgcgtccc cgcacattac cggtggaaaa
     6181 acatgctgac tggggtctgg tgtcgagcat ggcctttgcg atcgaggccg gtgtcgggct
     6241 ggcatgccag tactgggaca tgcttaccgt tgcgggggca gcacagttga cgccatggtc
     6301 cggcttcgtc gacagggtcg ggagtggaat tcccgagcgc gggtggaggc tggaccaggc
     6361 ggattgtttc ccgtttcact ttcgcaaagc gccgtaccct ggccatgcgt ggtgccgggc
     6421 tcgggcgctt cgaaagcacc tctctcggtg tgaaatgtaa catatcgtcg cggccagcct
     6481 gggtaggcga ggctggccaa agcgtccgcg accccgttcg cggcagggct ttcgccacga
     6541 gccacgcggg cgcgggcgat acaatagggc gtttgcgcgg cattcaccaa aatggggccg
     6601 cgcgggccgg ccgacctctc gagccgggtt ccgatcttcc cttatgaagg tagcagcgtg
     6661 tcctatcccg tttccgcgta tccgatcgag tcctataccc gtggcgccga attcgcccgg
     6721 ctgctggggt cacgcatcct gatactggat ggggccatgg gcaccatgat ccagcgctac
     6781 aagctcaccg aggcggattt ccgcggcgag cgcttcgcca atcattccaa ggacgtcaag
     6841 ggtgacaacg aactgctttc cctggtgcgc ccagacgtca ttctcgagat ccatcggcaa
     6901 tacctcgagg cgggctcgga cgtcatcgag accaacacct tcggcgccac ggccgtcgcg
     6961 cagggcgact acggcctggc ggacatcgcc tacgatctca acctagcctc ggcgaaactg
     7021 gcgcgccaag cctgtgacga gttcagcacg cccgacaagc cgcgtttcgt ggcgggcgcg
     7081 ctcgggcccc agcccaagac ggcgtccatc tcgcctgacg tcaacgatcc ggcggcgcgc
     7141 aacgtcacgt tcgaagaatt gcgcgtggcc tatgccgagc agctcaacgg gctgctcgat
     7201 ggcggcatcg atctggtcct gatcgaaacc atcttcgata cgctcaacgc caaggccgcc
     7261 attttcgcgg tggagtcggt gttcgaagag cgcggggtgc gcctccccgt catgatttcc
     7321 ggcacggtca ccgacgcatc ggggcgcatt ctttccgggc agacggtcga agccttctgg
     7381 aattcggtgc gtcatgcgcg gcccatcacg atcggcctga actgcgcgct gggcgcggca
     7441 ctcatgcgcc cgtacatcgc cgagctctcg aagatctgcg atacctacat ctgcgtctat
     7501 cccaacgcgg gcctgcccaa ccccatggcc gagaccggtt tcgacgaaac cccctgcgac
     7561 acgtcatcgc tgctcgagga a
//
LOCUS       DASTNK010000124        21026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1207807, whole genome shotgun sequence.
ACCESSION   DASTNK010000124 DASTNK010000000
VERSION     DASTNK010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 21026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21026
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207807"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFR20_08990"
     CDS             <1..277
                     /locus_tag="VFR20_08990"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEU0230696.1"
                     /translation="VRQVLGNRGTDQDASLTLRVHPGDLELVRRYLKSEHPEEKWRLC
                     PVESITPGGCLIHSSLGAVDATLETRWHNVAAALDVLPPPGQPPRGT"
     gene            309..1712
                     /gene="fliI"
                     /locus_tag="VFR20_08995"
     CDS             309..1712
                     /gene="fliI"
                     /locus_tag="VFR20_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HEU0230697.1"
                     /translation="MRLAADARRIPLVSPFTASGRVTRATGLVIEATGLRMAVGAACR
                     IEIAPGSDRWADAEVVGFHGQVLYLMPQSDISGLPPGARVLPAEPDKPVPGNATTQDR
                     RATSAQTRRLPVGETLLGRVVDGAGNPLDGLGPLRHAHMAPLTARAINPLSRAPIDTV
                     LDVGVRAVNGLLTVGRGQRMGLFAGSGVGKSVLLGMMARYTAADIIVVGLIGERGREV
                     KEFIEHNLGPEGLRRSVVVAAPADVSPLLRLQGAVYATRLAEYFRDMGRHVLLIMDSL
                     TRFAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGTQGDNGAGGSITAFYTV
                     LTEGDDQQDPIADSARAILDGHVVLSRGLAEAGHYPAIDIEASISRVMPSIVDGAQMR
                     RVHRFKQILSRYQRNRDLIAVGAYTAGNDPQIDEAVDRYPWLESYLQQGMDNRIDYAS
                     SARELAEVLGEKVEQHG"
     gene            1705..2190
                     /gene="fliJ"
                     /locus_tag="VFR20_09000"
     CDS             1705..2190
                     /gene="fliJ"
                     /locus_tag="VFR20_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HEU0230698.1"
                     /translation="MAEHASLGTLIDMAAKQVETAYRQLQQLMAERQDAERQLQALRT
                     YRLDYTLRLQNASEGGLSASNYHNFRRFIATLDDAITQQNKVMTQIEARLRGGREQWH
                     ARKQRLNSYETLLERDARARRYRENRNEQRSSDEIAMNLARQPFNTEQPDQDPATRRP
                     A"
     gene            2187..3506
                     /locus_tag="VFR20_09005"
     CDS             2187..3506
                     /locus_tag="VFR20_09005"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEU0230699.1"
                     /translation="MTSTSVIAVSASNISAKAPASSNAQPPGDSPAFRDVLANQSNPR
                     AANQAPAKNTDGQGSPVSSGNGTSTQAAGHPAKPEQAGKAVDDKKADDKKSIADAPSP
                     AGTGLPGIALYIAAEANAPLRQTVSSGEAHTPEGAQGVETARTATSASGRAHPGPAAI
                     AASTGDETPLPAPPVAIAPPASTNTTKQPEASPGNAATSPLAASAASGSGRPEHSSER
                     AGAASLAGTGNTPVHPVSESVKNTGQTHQTVAAQIPPMHAGINATPSPNGLPLVAQAA
                     PTHGLMATTASATGPAITIPLQNPRWPTELGRQFVAIAQSSQSGLGHTAQLRLDPPNL
                     GPLHIAIHVSDNVAQAVFFSPHAQVRQAVENALPQLQQQLAQAGIALGQASVSDQGAS
                     RQAFDDDAPSSRRRAAGVTTTVAVATGQPVAAGTRASAPNGLVDTFA"
     gene            3690..4187
                     /locus_tag="VFR20_09010"
     CDS             3690..4187
                     /locus_tag="VFR20_09010"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEU0230700.1"
                     /translation="MRKPLAYSQPRPMQRAIKTLLMLVLVAAASIGGTLFYSSHFGPF
                     TAHAIEAPKKPAPPPIPNPIYVPLDPFTVTLQGNDSRRILYTAITLRVADTESSKLLS
                     RYMPEVRNRVLLTLSEQNPDTVQTPEARAALAKALIKSLESPYIPQPLGPKISNVLFT
                     AFVVQ"
     gene            4188..5198
                     /gene="fliM"
                     /locus_tag="VFR20_09015"
     CDS             4188..5198
                     /gene="fliM"
                     /locus_tag="VFR20_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEU0230701.1"
                     /translation="MPYQNFLSQDEVDALLAGVTGEGDKPAEQDSLGNGVRPYDLSSP
                     ERVVRHRMQTLELINERFARRLRGVLLSFMRRNADITVGTLRVLKYSEFERNLPVPSN
                     LNMVTMKPLRGTALFSFDPSLVFLVIDSLFGGHGRYHTRVEGRDFTTTELRIIRRLLT
                     MTLECYGDAWQPVHPIELDYIRSEMHTKFANITSGNEIVVVSTFNIELGASGGKLNVC
                     LPYSMIEPVRDLLIRPLQHASANAIDRRWQQQLSRQVRSADVELIAEFASIPSSIAAL
                     MKMRAGDVLPFELSSIIQAHVGGVPVMECAYGTRSGHYALRVQKLLARDEPDTTDLNR
                     HD"
     gene            5191..5685
                     /gene="fliN"
                     /locus_tag="VFR20_09020"
     CDS             5191..5685
                     /gene="fliN"
                     /locus_tag="VFR20_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEU0230702.1"
                     /translation="MTDPIEDPAEKPDTADSADDWASALAEQNAQSKPATQADGLREG
                     ADDWASALAEQTAAGASGQVFKPLVGSGGGGDSDIDMIMDIPVQLSVELGRTRLTIRN
                     ILQLGQGSVVELEGLAGEPMDIFVNGYLIAQGEVVVVDDKYGIRLTDIITPADRLGRL
                     NSKR"
     gene            5689..6042
                     /gene="fliO"
                     /locus_tag="VFR20_09025"
     CDS             5689..6042
                     /gene="fliO"
                     /locus_tag="VFR20_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEU0230703.1"
                     /translation="MTEAAALRLILSLLAIIALILAGAWLARRSGWLKAGAGGSIRVL
                     GTHSLGARASLAVVQIEDARLVLGVTPGQISLLHTLPPAANPQTPDRDNVATLAKARP
                     ADFGAALRRALSRRP"
     gene            6111..6848
                     /gene="fliP"
                     /locus_tag="VFR20_09030"
     CDS             6111..6848
                     /gene="fliP"
                     /locus_tag="VFR20_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013972.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEU0230704.1"
                     /translation="MFAGLFLAIASLLPGTSLAQATLPAITALQNANGGQTYSLSIQT
                     LLLLTSLSFLPAALLMMTGFTRIIIVLGLLRSAMGTPTSPPNSVLIGLALFLTFFAMS
                     PVFNQIYSQAYQPLSAGTINFQTALERGAKPLKTFMLHQTREADLSMFANMANVGPLA
                     TPDAVPLRVLVPAFVTSELKSAFQIGFTIFIPFLIIDLVVASVLMALGMMMVPPVTIS
                     LPFKLMLFVLADGWHLLLGSLARSFYQ"
     gene            6861..7130
                     /gene="fliQ"
                     /locus_tag="VFR20_09035"
     CDS             6861..7130
                     /gene="fliQ"
                     /locus_tag="VFR20_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEU0230705.1"
                     /translation="MNSEAIMSMTYHAMKIALIMAGPLLLVTLIVGLVISIFQAATQI
                     NEMTLSFIPKLLAVGVALVLLGPWLISTMVDYMHQLFTSIPGLVS"
     gene            7144..7932
                     /gene="fliR"
                     /locus_tag="VFR20_09040"
     CDS             7144..7932
                     /gene="fliR"
                     /locus_tag="VFR20_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEU0230706.1"
                     /translation="MVSVELGQLYAWINAFIWPFFRIVALLSTAPVLGDSSIPVRIKL
                     AFAVALTVVIAPGLPTMPAVAPASFAGLGIAARQVLIGSALGLTMRIAFAAVQTAGEF
                     TGLQMGLSFASFFDPTTGANTAVLSRLMNIIAMLLFLALNGHLLVLVGLARTFEVLPI
                     SQAAMAPGGWQVLFDWSSQVLISGMLLALPLIIVLLAINLALGILNRTAQQLSVFAVG
                     FPITLMVGLILLAVVLPQSGPFLEHLFQSGYEAMGDLTGALAGR"
     gene            complement(8035..8553)
                     /locus_tag="VFR20_09045"
     CDS             complement(8035..8553)
                     /locus_tag="VFR20_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230707.1"
                     /translation="MKLAAFSKSGRGAYQDAPPHYPRFLLAAAAVILCVSTLSACSTT
                     GDSFRSAALSSMVPGRTTLAQASDLLGAAPVDTYAHGDGSVLARWAYKVSVVTDAIYA
                     RQELWLEFGPDGRFERVVRKVNVPSNPASSPAKEDTRVSLPATGASHPASAKPWGESM
                     ENTVIVYPLGSS"
     gene            complement(8553..10445)
                     /locus_tag="VFR20_09050"
     CDS             complement(8553..10445)
                     /locus_tag="VFR20_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU0230708.1"
                     /translation="MKFRHLQLKTSLSAIVFCLSLLTLVAVAIGWYNQQQNHQQLQTM
                     LAVGIKANNNVKKAYIDALLSIGKIDDASSAKSESDKAQALRVADGLLASSRLRLNLF
                     LKEDVDAADIKDSLKSAFSRYWALADTLTGLVKSGEHDKYEALRKEQLGSAAMIVGST
                     FSRFDLFIDSQNQAAMDSADQLSLRSNYACAALVVLAVILALMARLIVSRTVLRPLKR
                     VGRYFDHIAAGDLTQRIDVTSQNEIGALYTALRRMQDSLARMVGTVRVGTTQITVGAQ
                     SLVEGNVDLSGRTEEQAAALEQTAASMSQLAGTVKQNADNARQANRLAATASDVARRG
                     GQAVAEVVATMEGISGSSRKIADIVGVIDSIAFQTNILALNAAVEAARAGAQGKGFAV
                     VAAEVRALAQRSAQAAREIKGLIEDSVGKVREGSGQVARAGETMQEIVSSVTRVTDIM
                     EEIASASSEQSSGIDEINRAVAQMDEVTRQNMALVERAAQSASRLGERIREVTGAVAS
                     FKVADNQVIDVPAEAIPVPDETGAVAGSGREPTVLGRIASRNNAEAPGSTQPATAHRD
                     RRQGAGQRERNTSVEATGAGRKPDSSAPAGVPIRPAPRPAADPLRPSSAPVPATTADD
                     DEWVEF"
     gene            complement(10501..11736)
                     /gene="flgL"
                     /locus_tag="VFR20_09055"
     CDS             complement(10501..11736)
                     /gene="flgL"
                     /locus_tag="VFR20_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HEU0230709.1"
                     /translation="MRISSSLFFQTGLNSINAQQSDLMHLFQQIGSGQRMVTPADDPL
                     AAAQAINISQAQSLNTQYAANRGVAKTSLGTEENALNSVTTLMQDVKSRLVQAGNGTL
                     SDDDRATLANVLSNAKASLLALANSTDGNGQYLFSGMQGTSAPYAQDPTGKVTWQGDA
                     GQRNIQVDQTRQLPSGDVGVDVFQRATPGTNTYLTAAATTNTGTGIISSPQVTDPHGA
                     NVGSSFSIAFTSPTAYTVTVTAPDGATSTYAGAYSPGNTSIALNGGVQVNFSGQPATG
                     DTFTVQPAASSGVDLNIFDSFDSIISALKAPVNGDPAAQAKLSNTLASVLQRTGVNYN
                     NILTVRSSIGARLNEIDAIDANGSQRGLSYSSQLSSLEDLDYYSASANLQLRQSALQA
                     ATDAFRKIQSTNLFSSGTN"
     gene            complement(11802..13481)
                     /gene="flgK"
                     /locus_tag="VFR20_09060"
     CDS             complement(11802..13481)
                     /gene="flgK"
                     /locus_tag="VFR20_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEU0230710.1"
                     /translation="MNLANLGLSGLTAAQARLMTTGHNINNAATTGYNRQSVLVSTAG
                     ATSTSSGYIGRGVQVDGVQRSYDGFLYNQLVDSQSQGAAIASYGNQITQVNNLFGDRT
                     VGLNPALQNFFNGIQSVASTPGDSAARQELLGQAGSLAGQFNSINNFLDAQRQDVNTQ
                     VSTVVTQINSYANQVNDLNQQITKALASNPGQPPNDLLDQRDQAVSQLSQLINVKTVE
                     QDNVVSLTVGNGQVLLGGGTVYPLQAVGSAADPSRTVVAYTAVSGPGGATTPVEMDES
                     GITGGSLAGLLSYRSQALDPVQNAVGRMAAGLAMTVNHAHEQGVDLNGNPGKAMFSLS
                     APKVIPNSANGSTAEITVQFQDLATNPDAAAQLTGQDYKIAFDGTNYTVTRVQDGSQV
                     YSGATLSNQSIDGLSFNLTGSGTAPQAGDSWLVQPTRDTAGGFTLALSDPAQIAAADA
                     TGGSANGNNALSLAKLQTDKTLDGGTMSVTDAFSRIVNQVGVMTQQNSASAKAQNTLI
                     QQNYSAQQNVSGVNLNEEYVNLDQFQQQFQAASRLIDVSSTLFDTLLGLAK"
     gene            complement(13601..14587)
                     /gene="flgJ"
                     /locus_tag="VFR20_09065"
     CDS             complement(13601..14587)
                     /gene="flgJ"
                     /locus_tag="VFR20_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HEU0230711.1"
                     /translation="MSLVRYIHMPEAASSSIFDFSNLDSLKQGVAAGANAPEAEQKKV
                     AHQFEALFIQQFLKQARQASTLSGPFDSEQTKMAQGMADEQLALQLANPGIGLAKALL
                     AQIQAGGSHSGKTGEADALAATSSASARVSGMRSQLGMGGPDSVPSISALISMLNGGA
                     LAVGTVKAAQEAPQKIRDFVSRMSGAAHLASNQSGVPAKLILSQAALESGWGEHEIRG
                     QDGQDSHNLFGIKATAGWKGKVVNVMTTEYVDGEARKVVQPFRAYDSYADAFADYAKL
                     IGQSPRYQDVVNAPTAEDAARQIQNAGYATDPSYASKLISIMGYFDAAGTHG"
     gene            complement(14600..15748)
                     /locus_tag="VFR20_09070"
     CDS             complement(14600..15748)
                     /locus_tag="VFR20_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEU0230712.1"
                     /translation="MSLFRTVRCFSSLRCQARRVAVALALGLLALPAAHAERIKDIAS
                     IQGVRANQLVGYGLVVGLDGSGDQVRQTPFTQQSLTNMLSQLGVTVPQGTNMQVKNVA
                     AVMVTARLPAFSRPGQTLDVVVSSMGNAKSLRGGTLLMTPLKGANGQVYAMAQGNLLV
                     GGAGAQSGGSKVQVNQLNGGTIPGGAIVERDVPTTYAHDGVIRLELNNTDFGVVNNVV
                     AALNRAMGPETASAVDGRVIRLRGPLDPSAQAGFLSQVENLQVRMPPVRAKVIINART
                     GSVVMNSTVTIDEAAVTYGNLSVVISNQQQVSQPNTPFAGGQTVVTNNTQIAMSTSNG
                     TIKHMRTSANLADVVRALNALGATPQDLLSILQTLKSAGALHADLEII"
     gene            complement(15764..16450)
                     /locus_tag="VFR20_09075"
     CDS             complement(15764..16450)
                     /locus_tag="VFR20_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEU0230713.1"
                     /translation="MQKGFRAMAACAALAVVSGCALVPPEPIVTGPLTAAPPPRLPPP
                     VPNGSIYQPTAYGDYPLFEDRRPRNVGDIVTIIIQEKTNAAKNVDTATNRTSSASLGM
                     DVMPAAILPNSLNSKQDMNASGGNKMEGKGSSSAANTFSGTITTTVVGVLPNGNLQIA
                     GQKQIAINRGSEYVRFSGVVDPRSITGSNTVLSTQVADARMEYRSKGTMDEVQTMGWL
                     QRFFLNISPF"
     gene            complement(16469..17254)
                     /gene="flgG"
                     /locus_tag="VFR20_09080"
     CDS             complement(16469..17254)
                     /gene="flgG"
                     /locus_tag="VFR20_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEU0230714.1"
                     /translation="MIRSLWIAKTGLEAQQTNMDVISNNLANVNTDGFKQSRAVFQDL
                     MYQTLRQPGAQVGDSNQLPSGLQIGTGVRTVATERIHTQGNLKETDNPMDIAIQGRGF
                     LQVQMPDGTFRYTRDGSLQVDQNGQLVTSGGYPIQPPINIPDNALSLTIARDGTVSVT
                     QPGAVGTNVNVGQLQLATFVNPTGLQSEGENLYAETDASGPANYEQPGLDGAGLVLQK
                     YVETSNVNVAEELVNMITTQRAYEMNSKAIQTSDQMLARLTQM"
     gene            complement(17316..18071)
                     /gene="flgF"
                     /locus_tag="VFR20_09085"
     CDS             complement(17316..18071)
                     /gene="flgF"
                     /locus_tag="VFR20_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HEU0230715.1"
                     /translation="MDRIIYTAMGGAQRTLEQQAVISNNLANASTDGFRRQLAIYRSV
                     PVESPDTLPTRVGTVTSTPGSDFQQGAMRETGRPLDVAITGQGWFSVQTPSGEAYTRG
                     GSFAVNAQGQLVTAQGGLPVLGDNGGPIQVPERGRVTFTGDGAVDVLGAGDNPTEVQQ
                     IGRLKLVDPPTSQLSQGADGLFRVGPGGGVPAQADPNVRIVSGFVEQSNVSPAQAMVG
                     MISNSRRFDMQMKVISDAGANEDQGNSILAPAK"
     gene            complement(18079..19293)
                     /gene="flgE"
                     /locus_tag="VFR20_09090"
     CDS             complement(18079..19293)
                     /gene="flgE"
                     /locus_tag="VFR20_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HEU0230716.1"
                     /translation="MGFGQGLSGLNAASQDLDVIGNNIANSGTVGFKSGAATFADVFA
                     NSSVGLGVQVASVNQNFSAGTVTNTGNQFDMAIDGANGLFVVQDPSGNTFYSRNGQFH
                     ADKDNYIVNSQGQRLMGYDTTGNNLAPVKVPVGNVAPTATSTASFTTNLDANAAVIDP
                     AVTPFDITNSSSYTYQRSMTVYDSLGNAHQLTQYFAKRAPSGTNSVWDVNYRLDGAVV
                     GSPADGAQKLTFNSAGAIVTSPPTATVTIAAPGGAGSPAAPMSVAMDYSGSTQFGSDF
                     AANFHQNGYYSGEYASMSIAGDGSIVANYTNGETQTLGTLALANFNNLQGLKPVGGNA
                     WAQTADSGQPILGRPGSNGLASVKGQAVEESNVDMSQELVKMIIAQRTYQANAQTIKT
                     QDQVMQTLISGI"
     gene            complement(19386..20093)
                     /locus_tag="VFR20_09095"
     CDS             complement(19386..20093)
                     /locus_tag="VFR20_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018573990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HEU0230717.1"
                     /translation="MTSTNGVPATSASNGTAALAAASASTNSLMSDTQGQFLTLLVTQ
                     LQNQDPLNPMDNAEVTSQIAQLSTVNGINQLNNTLLALSGQMDVSQSMQAAGLIGKGV
                     LVPGTKVSLGSDPTNPTVKQTTPFGVDVISPAAKVVVTIMDSSGKAVRQIDLGALPAG
                     VQAVSWDGMGDGGAPVPDGAYNIKVTALDGQGGPVSADALTYGKVSSVQYSSSGPKID
                     LGLAGSASLSDIRQILS"
     gene            complement(20145..20555)
                     /gene="flgC"
                     /locus_tag="VFR20_09100"
     CDS             complement(20145..20555)
                     /gene="flgC"
                     /locus_tag="VFR20_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEU0230718.1"
                     /translation="MMSILSIAGSALEAQSQRMNVSASNLANADSAVGPDGQPYRARE
                     VIFQTQPHADQSGPAAIGGVRVAGVVESSAPPKLKYDPGNPLADERGYVTMPNVDVVA
                     ETVNMISASRSYQANVDVINTAKSLMAQALTIGQ"
     gene            complement(20574..20981)
                     /gene="flgB"
                     /locus_tag="VFR20_09105"
     CDS             complement(20574..20981)
                     /gene="flgB"
                     /locus_tag="VFR20_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEU0230719.1"
                     /translation="MADPIGSDLSFYQAAIALRQRRQEVLASNIANSDTPNYKARDFD
                     FDKALQNALGDDMRLPDTHLTLTSPRHIPGQATSSGPVDLLYRVPTQPSLDGNTVDMN
                     TERVAFAENTMHYQADLDLVSQRIKTMLLALQQ"
BASE COUNT         3797 a         6875 c         6349 g         4005 t
ORIGIN      
        1 cgtccgccag gtgctgggca accggggcac cgaccaggat gcgtccctga ctctacgcgt
       61 gcaccccggt gacctcgaac tcgtgcgccg ctacctgaag agcgagcacc ccgaggaaaa
      121 atggcgcctg tgcccggtgg aatccatcac gcccggcggc tgcctcatcc actcatcgct
      181 gggcgcggtg gacgccacgc tggaaacccg ttggcacaac gtcgccgccg cgctcgacgt
      241 ccttccgcct cctggacagc cccctcgtgg aacctgacca caccgacacg cccggcgcgc
      301 gctggcggct gcggctggcg gccgacgcca gacgcatccc gctcgtgtcg ccattcaccg
      361 ccagcggcag agtgacacgc gccaccggcc tggtgatcga ggccacaggg ctgcgaatgg
      421 ccgtcggcgc cgcatgccga atagaaatcg caccgggaag cgaccgttgg gcggacgcgg
      481 aagtggtcgg cttccacggc caagtcttgt acctgatgcc ccagagcgac atctcggggc
      541 tgccccccgg cgcacgcgta ctacccgccg agcctgacaa acccgtaccg ggaaacgcca
      601 cgacacaaga cagacgggcc acgtcagcgc aaacccggcg ccttccggtc ggggaaacct
      661 tgcttggccg tgtcgtggac ggtgcgggca acccgctgga tgggctcggc cccctgcggc
      721 atgcccacat ggcccccttg acggcacggg caatcaaccc cctctcccgc gcgcccatcg
      781 acacggtgct cgatgtggga gtgcgcgccg tcaatgggtt gctgaccgtt ggtcgcggcc
      841 aacgcatggg gctgttcgcc ggctccgggg tcggcaagag cgttctgctg ggcatgatgg
      901 cgcgctatac cgcagccgac atcatcgtcg tggggctcat cggcgaacgc ggccgtgagg
      961 tcaaggagtt catcgagcac aacctgggac ccgaggggct gcggcgctcg gtcgtggtgg
     1021 cggcgccagc cgatgtctcg cccctgctgc gcctgcaagg tgccgtctat gccacccgtc
     1081 tcgccgagta cttccgcgac atggggaggc acgttctgct gatcatggat tcgctcactc
     1141 gctttgccat ggcgcaacgc gagatcgcgc tggcgatcgg cgaaccacca gccaccaaag
     1201 gctacccacc atcggtattc gccaaactgc cggcactggt ggagcgcgct ggcaatggca
     1261 cccaaggcga caatggagca ggaggatcga tcactgcgtt ctataccgtc ctcaccgagg
     1321 gggacgatca acaagatccg atcgccgatt cggcgcgcgc catcctggac ggccacgtcg
     1381 tcctgtcacg cggcttggcg gaggcgggcc attaccccgc catcgacatc gaggcgtcca
     1441 tatcgcgtgt gatgccgtcc atcgtcgacg gcgcacaaat gcggcgggtt catcgcttca
     1501 agcaaatcct gtcgcgctat cagcgcaatc gggatctgat cgcggtgggc gcctacacgg
     1561 cgggcaacga cccgcagatc gatgaggctg tcgaccggta tccctggctg gaatcctatt
     1621 tgcaacaagg catggacaac cgcatcgatt acgcatcctc ggcccgcgaa ctggctgagg
     1681 tccttggcga aaaggtggaa caacatggct gaacacgcgt ccctgggaac cctgatcgac
     1741 atggcggcca aacaggtgga aacggcttac cgccaactcc agcaactgat ggctgagcgc
     1801 caggacgccg aacgtcaact gcaagccttg cggacgtacc gtctcgatta cacgctgcgc
     1861 ctgcaaaacg cctccgaagg aggcctttcg gcgtcgaatt accacaattt ccggcgcttc
     1921 atcgccactt tggacgacgc gatcacgcag caaaataagg tcatgacaca gatcgaggcg
     1981 cggctgcgcg gcgggcgtga gcaatggcac gcacggaaac agcgcctgaa ctcctacgaa
     2041 accctgctgg aacgcgatgc gcgcgcgcga cgataccggg aaaaccgcaa cgagcagcga
     2101 tccagcgacg aaatcgccat gaatctggcg cggcagccgt tcaacacgga acagcccgac
     2161 caggatccgg ccacgaggcg ccctgcatga cgtccacatc ggttatcgcc gtctccgctt
     2221 ccaatatatc ggcaaaggcg ccggcctcgt ccaacgcgca accgcccggc gacagcccgg
     2281 cctttcgcga cgtgctggcc aaccagtcga acccgcgcgc cgccaatcag gcgcccgcga
     2341 agaacaccga tgggcaggga agcccggtgt cgtctggcaa tggcacatcc acgcaagcag
     2401 ccggccaccc cgccaaacca gaacaagccg gcaaagccgt cgatgacaag aaagccgacg
     2461 acaaaaagtc catcgctgat gcgccgtccc cggcgggcac cggtctgccc ggcatcgcgc
     2521 tctatatcgc cgccgaggcc aatgcccccc tgcgccagac ggtttcttcg ggcgaagcgc
     2581 acacgccaga aggcgcacag ggcgtcgaaa ccgcccgcac cgccacgtcg gcttccggtc
     2641 gcgcacaccc tggccccgca gccatagcgg caagcacagg cgatgaaacg ccgctccccg
     2701 cgccgcctgt ggcaatcgcc cccccggcgt cgaccaacac caccaaacag ccagaggctt
     2761 ctcccgggaa cgcggcaacc agtccgctgg cggcatccgc cgcgtcgggc agcggccgtc
     2821 cagagcactc ctctgaacgg gcgggcgccg catcactcgc gggaaccggc aacacgccgg
     2881 ttcacccagt atccgagtcg gtaaaaaaca ccgggcaaac ccaccagacc gtggccgcgc
     2941 agattccccc catgcacgcc gggatcaacg ccacgccatc gcccaatggg ctgccactcg
     3001 tggcacaagc cgctcccaca catgggttga tggcgaccac ggcctccgcc acggggccgg
     3061 cgatcaccat tccgctgcaa aaccctcgat ggccaaccga actcggacgc caattcgttg
     3121 ccatcgcgca gtccagccag tcgggcttgg gtcacacggc acagctacga ctggatccac
     3181 ccaacctcgg cccgctgcac attgccatcc acgtcagcga caacgtggcg caagccgtgt
     3241 tcttctcccc tcatgcccaa gtgcggcagg ccgtggagaa cgcattaccg cagttgcaac
     3301 agcaactcgc ccaggccggc attgcgctgg gccaagccag cgtgagtgat cagggggcct
     3361 caaggcaggc attcgacgat gacgcgccct cctctagaag acgggctgcc ggcgtcacca
     3421 caaccgttgc cgtggccacg ggccagccgg tggcggccgg aacccgcgca tcggctccga
     3481 acggcctggt cgacaccttc gcctgacgga gtgctcaacc gctcatcaac caaacgccgg
     3541 agatgcccgc ctttcgacct gttgttcatg aacaccgatg cccccacgct cgggcacaat
     3601 gattcccgca cgcaacggaa caccctccgc ggccgatgcg tgaagcgcgg accgcggcat
     3661 gcttccggcg tcgaaccccg agcaccgcta tgcgaaaacc tttggcctat tcacaacccc
     3721 gccccatgca aagagccatc aaaaccctgc tgatgctcgt actcgtcgcc gccgccagta
     3781 tcggcggcac gctcttctat tcatcccatt tcggcccctt caccgcccac gccatcgaag
     3841 cgcccaagaa gccagcgcct ccgcccatcc ccaacccgat ttacgtcccg ctcgaccctt
     3901 tcaccgtcac gctccagggc aacgacagcc ggcgcattct gtacacggcg atcacgctgc
     3961 gggtcgcgga cactgaatcc agcaaactgc tgagccgcta catgcccgag gtgcggaacc
     4021 gtgtcctgct gacgctgtcc gaacagaacc ccgacaccgt gcagaccccc gaggcgcgcg
     4081 ccgcgctagc caaggccctg atcaaatcgc tcgagtcgcc ttacatcccg cagcccttgg
     4141 gaccgaagat ctccaacgtt ctgttcacgg cattcgtggt gcaataaatg ccttatcaga
     4201 attttctctc gcaggacgaa gtcgacgccc tcttggcggg cgtgacgggc gagggggata
     4261 aacccgcaga acaagactcc ctggggaacg gggtacggcc ttatgacctg agttcaccgg
     4321 aacgggtggt gcgccaccgc atgcagacgc tcgaactgat caacgagcga ttcgcacgcc
     4381 gcctgcgcgg cgttctgctc agtttcatgc gccgcaacgc tgacatcacc gtcggcaccc
     4441 tgcgggttct gaaatactcg gaattcgaac gcaacctgcc ggttcccagc aatctgaaca
     4501 tggtgaccat gaagccgctg cgcggcacag cgttgttctc cttcgatccc agcctggttt
     4561 tcctggtcat cgacagcctg ttcggcggcc atgggcgcta ccacacgcgt gtagagggcc
     4621 gggacttcac caccaccgaa ctacgtatca tccggcgctt gttgaccatg acgctggaat
     4681 gctatggcga cgcctggcag cccgtgcatc cgatcgaact ggactacatc cggtcggaaa
     4741 tgcacaccaa gttcgccaac atcaccagcg ggaacgaaat cgtggtggta tccacgttca
     4801 acatcgaact tggcgcttcc ggaggcaaac tcaatgtctg cctgccctat tccatgatcg
     4861 agcccgtgcg cgacctgctc atacgccccc tgcagcacgc cagcgccaac gcgatagacc
     4921 gccgctggca gcaacaactg tcgcgccaag tgcgcagtgc agacgtggag ctgatcgccg
     4981 aattcgcaag catccccagc agcatcgccg cattgatgaa gatgcgcgcc ggcgacgtcc
     5041 tgcccttcga gctgtcgtcc atcatccagg cgcacgtggg tggcgtgccg gtcatggaat
     5101 gtgcctatgg cacccgcagc ggtcactatg ccctgcgggt ccagaaactc ttggcccgcg
     5161 acgaacccga cacaaccgac ctcaaccgcc atgactgatc ccatcgaaga ccccgccgaa
     5221 aagcccgata cagcggattc cgccgacgac tgggcatccg ccctggccga gcagaacgcg
     5281 caatccaaac cggcgacaca ggccgatggc ctgcgcgaag gcgctgacga ctgggccagc
     5341 gcactggcgg aacaaaccgc ggccggcgca tccggccagg tgttcaagcc actggtcggc
     5401 agcggcggcg gcggggacag cgacatcgac atgatcatgg acatcccggt gcaactgtcg
     5461 gtcgaactcg gccgcacgcg gctgaccatc cggaacatcc tgcaactggg ccagggatcg
     5521 gtcgtcgaac tggaagggct ggctggcgaa cccatggaca tcttcgtcaa cggctacctg
     5581 atcgcccagg gggaagtcgt cgtggttgac gacaaatacg gcattcgtct cacagacatc
     5641 atcacccccg ccgatcgcct cggccgtctg aactcgaaac gctgaaccat gaccgaagcc
     5701 gccgcgctgc gccttattct cagcctgctg gcgatcatcg ccctgatatt ggcgggcgct
     5761 tggctcgcgc gccgttccgg ctggctgaaa gcgggcgcgg gcggctcgat ccgtgtcctg
     5821 ggcacacaca gcctgggcgc gcgcgccagc ctcgcggtcg tccagataga ggacgcgcgt
     5881 ctggtcctgg gcgtgacacc cggacagata tccctgctgc acacgctgcc gcccgcggcg
     5941 aacccgcaaa caccggaccg ggacaacgtt gccaccttag ccaaagcacg ccccgcggat
     6001 ttcggcgccg cgttgcggcg cgccctctcc cgccgtccgt gagcggccgc cccatgcttg
     6061 ctccgcccca ccttcgcttc gctcccgccc ggcggcgccc agccatcgtc atgttcgccg
     6121 ggctgttcct ggccatcgcc agcctgttgc ccggaaccag tctggcgcag gcgacgctgc
     6181 ccgccatcac cgccctccag aacgcaaacg gtgggcagac atattccctg agcatacaga
     6241 cgctgctgct gttgacatcc ctatcgttcc ttcccgccgc attgctgatg atgacgggct
     6301 tcacccgcat catcatcgtg ctcggcctgc tgcgcagcgc catgggcacg ccaacgtcgc
     6361 caccgaattc cgttttgatc gggctggcgc tgttcctgac tttcttcgcg atgtcgccgg
     6421 tgttcaatca gatctacagc caggcctatc agcccctgag cgccggcacc atcaactttc
     6481 agacggcact ggaacgcggc gccaaaccac tcaagacatt catgctgcac caaacgcgtg
     6541 aagccgatct ttccatgttc gccaacatgg ccaatgtcgg cccgcttgcc acgccggacg
     6601 ctgtgccctt gcgtgtattg gtgccggcct tcgtcacgag cgagctgaag tccgcattcc
     6661 agatcggctt caccatattc atcccatttc tcatcatcga cctggtcgtg gccagtgtgc
     6721 tcatggcgtt gggcatgatg atggtgccgc ccgtgacaat ctctctgccg ttcaaactca
     6781 tgctgttcgt cctggccgac ggctggcacc tgctgctcgg ctccctggcg cgcagtttct
     6841 atcagtaggg aggaacgtga atgaattccg aagccatcat gtcgatgacg tatcacgcca
     6901 tgaagatcgc cctgatcatg gcgggtccgc tgctgttggt cacactgatc gtgggactgg
     6961 tgatcagcat cttccaggcg gccacgcaaa tcaacgagat gacattgtcg ttcataccaa
     7021 aactgctggc tgtcggcgtg gccctagtat tgctgggccc atggctcatc tccaccatgg
     7081 tcgactacat gcatcaactg ttcaccagca tacccgggct ggtgtcctga cgcgcgcggg
     7141 atagtggtct ccgtcgaact cgggcagcta tatgcctgga tcaacgcctt catctggccg
     7201 ttcttccgca tcgtcgcgtt gctgagcacc gcgccggtgc tgggcgactc gtctataccc
     7261 gtgcgcatca aactggcctt cgcggtcgca ctcaccgtgg tgatcgctcc agggctgccc
     7321 accatgccgg cggtcgcgcc cgcatcattc gccggtctgg ggatcgccgc gcgccaagtg
     7381 ctcatcggct ccgccctggg actgaccatg cgcatcgcct tcgcggcggt gcagaccgca
     7441 ggcgaattca ccgggctgca aatgggcctg tcgttcgcct cgttcttcga ccccacgacc
     7501 ggcgccaaca ccgccgtgtt gtcgcgcctg atgaacatca tcgcaatgct cttgttcctc
     7561 gcgctcaacg gtcatctgct cgtgctggtt ggtctggcgc gcaccttcga agtgctgccc
     7621 atcagtcagg ccgccatggc gccgggaggc tggcaggtat tgttcgattg gagttcccag
     7681 gtgctgatct cgggcatgct gctggccctg ccgctcatca ttgtgttgtt ggcgatcaat
     7741 ctggccctgg gcatcctcaa ccgcaccgcc cagcaactat cggtgttcgc cgtgggcttt
     7801 cccatcaccc tgatggtggg tctgatcctg cttgccgtgg tgctgccgca aagcggcccg
     7861 ttcctcgagc acttgttcca atccggttat gaggccatgg gcgatctgac tggggccctg
     7921 gcgggtcgct gatgccccgt ttgtttggcg ggttcgacta ttcaaaatgc acgagccagc
     7981 caagccgcgc acccgtcatg gaacagaagg aacccagggt cgcgcccgcg aagctcagga
     8041 agaccccagc gggtagacga tcaccgtgtt ctccatcgac tcgccccatg gcttggcgga
     8101 ggcgggatgc gaggcgccgg ttgctggcag ggatacccga gtgtcctcct tcgctggcga
     8161 agaagcggga ttcgacggca cgttcacctt gcgcacgacc cgttcgaagc ggccatcggg
     8221 gccgaactcc agccagagtt cctgcctggc gtagatcgcg tcggtcacca ccgaaacctt
     8281 gtaggcccag cgcgccagca ccgacccatc accgtgcgcg taggtgtcga ccggtgccgc
     8341 acccagcaga tcgctggcct gcgccagagt cgtgcgaccc ggcaccatgc tcgacaacgc
     8401 ggccgaacgg aaactatcgc cggtggtgct gcaggcggac aaggtggaca cgcagagaat
     8461 cacggccgcc gccgcgagca ggaaacgtgg gtaatgaggt ggcgcatcct gatacgcgcc
     8521 acgcccgctc ttggaaaacg ccgccaattt catcagaact ccacccactc gtcgtcgtca
     8581 gccgtggtcg cgggcaccgg ggcggacgac ggcctcaacg gatcggcagc tggtcgggga
     8641 gcgggccgaa tgggcacgcc ggcgggtgcc gaggaatcag gctttcttcc ggctcccgtg
     8701 gcttcgacag atgtattccg ttcgcgttgc cccgcgcctt ggcggcgatc tcgatgtgcg
     8761 gtggccggct gtgtcgatcc cggcgcttca gcgttgtttc gagaggcgat tcgtccgagg
     8821 acggtgggtt cccgcccgga tccggccacc gcgcccgttt cgtcgggaac cggaatcgct
     8881 tcggccggca cgtcgatcac ctgattgtcg gccaccttga acgaggccac cgcgccggtg
     8941 acctctcgaa ttcgctcgcc cagacgcgag gcggattggg cggcgcgctc aaccagcgcc
     9001 atgttctgcc gcgtcacctc gtccatttgc gccaccgcgc gattgatttc gtcgataccc
     9061 gacgactgct cgctcgatgc gctggcgatt tcctccatga tgtcggtgac ccgcgtcacc
     9121 gacgacacga tctcctgcat ggtctcgccg gcgcgcgcca cctggcccga gccttcccgc
     9181 actttaccaa cggagtcttc gataaggcct ttgatctccc tggccgcctg ggcgctgcgc
     9241 tgggcaagcg cccgaacctc ggcggcgaca accgcgaacc ctttgccctg cgccccggca
     9301 cgggccgcct cgaccgccgc gttcaaggcc aggatgttgg tctgaaaggc gatgctgtct
     9361 atgaccccca cgatgtcggc gatcttccgt gaactgccgg aaatgccctc catggtggcc
     9421 acgacttcgg cgactgcctg gccgccgcgc cgagccacgt ccgacgcggt cgccgcgagc
     9481 cgattcgcct ggcgggcatt gtcggcgttc tgcttcactg taccggcaag ttgactcatg
     9541 ctggccgccg tctgctccag cgccgccgcc tgttcttcgg tgcgcccgct gagatcgaca
     9601 ttgccctcga caagagactg ggcgccgacg gtgatctgcg tggtcccgac acgcaccgta
     9661 ccgaccatcc tggcgagact gtcctgcatg cgccgcagtg ccgtgtaaag cgcgccgatc
     9721 tcgttctgcg acgtgacgtc gatacgctga gtgaggtcgc ccgccgcgat gtgatcgaaa
     9781 taccgtccca cccgcttgag cggacgcaac actgtgcgtg acacaatcag cctcgccatc
     9841 agcgccagga tgacagccag caccaccagc gcggcgcagg cgtagttgct gcggagcgac
     9901 aactggtcgg cggagtccat ggcagcctgg ttctgcgaat caatgaaaag gtcgaaacgc
     9961 gagaacgtgg aacccacgat catggccgcc gaacccaact gttccttgcg cagcgcctca
    10021 tatttgtcgt gttcgccaga cttgaccagc ccggtcaagg tgtcggccag agcccagtag
    10081 cgcgagaacg ccgatttcaa tgagtccttg atgtcagccg cgtccacgtc ttccttcagg
    10141 aagagattca ggcgcaatct ggaacttgcc aacaggccat ccgccacccg caacgcctgc
    10201 gccttgtcgg actcggactt ggccgacgat gcgtcatcga tcttgccgat ggacagcagc
    10261 gcgtcgatat aggccttttt gacgttgttg ttggctttta ttccgacggc caacatcgtt
    10321 tggagctgct gatggttctg ttgctggttg taccagccga tggcaaccgc caccagggtc
    10381 agcagcgaca gacaaaacac tatcgccgac aaactggtct tcagttgcaa atgcctgaac
    10441 ttcatgtgaa tacctttttg tgtggacagg ctttggcgcg ccgctccgcg ggacgacgcg
    10501 ctaattcgtt ccagaactga ataggttggt ggactggatt ttgcggaagg cgtcggtcgc
    10561 cgcctgcagc gcggactggc gcaactgcag gttggccgaa gccgagtaat aatccagatc
    10621 ttccaggctg gagagctgcg aggaatagct cagcccgcgc tgggaaccat tggcgtcgat
    10681 cgcgtcgatt tcattcaggc gcgcgccgat agaggagcgc actgtcagga tattgttgta
    10741 attgacacct gtgcgctgca acacgctggc cagcgtattg ctcagcttcg cctgcgcggc
    10801 cggatcccca ttgacggggg ctttcagggc agagatgata ctgtcgaacg aatcaaagat
    10861 gttcaggtcc acgccactcg aggcggctgg ctgaacagtg aacgtgtcgc ctgtcgcggg
    10921 ctggccggaa aaattgacct gcacgccgcc attgagcgcg atgctggtat tgcctggaga
    10981 ataggcgccg gcgtacgtgc tggtggcgcc atcgggtgcg gtcacagtga ccgtgtacgc
    11041 cgtcgggctg gtgaaagcga tagagaagct gcttcccaca ttggcaccgt gcggatcggt
    11101 gacctgcggt gaactgatga taccggttcc cgtattggtg gtggccgccg ccgtcagata
    11161 cgtgttggtt cccggcgtgg cgcgttggaa cacgtccacc cccacgtcac cacttggcag
    11221 ctgccgcgtc tgatcaacct gaatgttccg ttggcccgca tcaccctgcc aagtgacctt
    11281 tccggtggga tcctgggcat acggcgcgct ggtgccctgc atacccgaga ataggtactg
    11341 gccgttcccg tcggtactgt tggccagcgc caggaggctg gccttcgcgt tcgacagcac
    11401 gttcgccaag gttgcgcgat catcgtcaga cagtgtgcca ttgccggcct gaaccagacg
    11461 cgacttcacg tcctgcatca gtgtggtgac cgaattcagc gcgttctctt cggtgcccag
    11521 gctggtcttg gcgacgccgc gattggccgc gtactgagtg ttgagcgatt gcgcctggct
    11581 gatattgatg gcttgcgccg ccgcaagggg atcgtcggcc ggtgtcacca tgcgctgacc
    11641 actgccgatt tgctggaaca gatgcatcag atcggactgc tgggcgttga tggaattgag
    11701 cccggtctgg aaaaaaagag aagaactgat gcgcatggct gcaacctcgg gttcggcgac
    11761 gtcgccggaa aggagtggga acgattgacc cgttgaatgc cttatttcgc aagacccagc
    11821 aaggtatcga acaacgtgga gctgacatct atcaaacgcg aagccgcctg gaactgttgc
    11881 tggaactggt caaggttcac atattcttcg ttcaggttga cacctgacac gttctgctgt
    11941 gccgaataat tctgctggat aagcgtgttc tgcgccttgg ccgatgcgga attctgttgg
    12001 gtcatgaccc ccacctgatt cacgatccgc gagaaggcat ccgtcacgct catggtgcca
    12061 ccgtccagcg tcttgtccgt ctgtagtttg gccagactca gggcattgtt tccgttggcc
    12121 gacccgcccg tggcgtccgc cgccgcgatc tgagccggat cggacagggc cagcgtgaag
    12181 ccgcccgccg tatcacgcgt cggctgcacc agccatgagt cacccgcctg aggagcggtg
    12241 cccgagccgg taaggttgaa agacaggccg tcgatgctct ggttgctcag tgtcgccccg
    12301 ctgtacacct gggagccatc ctgaacgcgg gtaacggtgt agttggtgcc gtcgaaggcg
    12361 attttgtaat cctgcccggt aagctgggcg gccgcatccg ggttggtggc caaatcctgg
    12421 aactgcacgg tgatctcagc ggtgctgccg tttgcactgt ttggaatcac tttcggcgcg
    12481 ctcagggaga acatcgcctt gcccggattg ccgttgagat ccaccccttg ctcatgggca
    12541 tgattgaccg tcatggccag gcccgccgcc atgcggccaa cggcgttctg caccggatcg
    12601 agcgcctggc tgcgatatga caacaacccc gccaggctgc cgcccgttat gccggattca
    12661 tccatttcga ccggtgtggt cgcaccgcca gggccggaca ccgccgtata ggcgaccacg
    12721 gtccgcgatg gatcggccgc cgaccccacg gcctgcagag gatacactgt gccgccaccc
    12781 agcaacacct ggccatttcc caccgtcagg ctgacgacgt tgtcctgttc gactgtcttg
    12841 acgttgatga gttgactcag ttgcgagacc gcctgatcac gttggtccag cagatcattg
    12901 ggaggttggc cgggattgct ggccagagcc ttggtgatct gctgattcag gtcattgacc
    12961 tgattcgcat agctgttgat ctgcgtgacg acggttgaaa cctgggtatt gacgtcctgg
    13021 cgctgcgcat cgaggaagtt gttgatggaa ttgaattggc cggccaggct gccggcctgc
    13081 cccagcaatt cctggcgcgc ggcgctgtcg ccaggcgtgg acgccacaga ctggatgccg
    13141 ttgaagaagt tttgcagcgc gggattcagg cccaccgtgc ggtcgccgaa cagattgttg
    13201 acctgggtga tctgattgcc atacgacgcg atggccgcgc cctggctttg ggaatcgacc
    13261 aactggttat acaggaaacc atcgtaggag cgctgcacgc cgtcgacctg cacgccacgg
    13321 ccaatgtaac cgctgctggt gctcgtcgcg ccggccgtgg aaaccaggac agactggcgg
    13381 ttgtaaccgg tggtggcagc gttgttgata ttgtggccgg tggtcatcag acgcgcctgc
    13441 gcagccgtga ggccggaaag gccgagattc gctagattca tgatttcgct cgcactgtga
    13501 tcaagccgcg ccaggacaag aaccaggagc cgccacggaa attaacggct cggggtggtg
    13561 aatatttagg cggggacccg acgggccgcc catcgtgtgg tcaaccgtgc gtgccagccg
    13621 catcgaaata ccccatgatg gaaatgagtt tgctggcata ggaggggtcc gtggcatagc
    13681 ccgcgttctg aatctgcctg gcggcgtctt ccgccgtcgg ggcgttcacg acgtcttggt
    13741 aacgcggact ctggccaatg agcttggcgt aatcggcgaa agcatccgca taggaatcgt
    13801 aggcgcggaa tggttgcacc actttccggg cttcgccatc gacgtactcg gtggtcatga
    13861 cattcaccac cttccccttc cagccagccg tggccttgat accgaacagg ttgtggctgt
    13921 cctgtccatc ctggccacgg atttcgtgtt cgccccatcc tgattccagc gcggcctggc
    13981 tgaggatgag tttggccggc acaccgcttt gattcgaggc cagatgggcc gcgcccgaca
    14041 tcctggacac gaaatcacgg attttctgtg gagcctcctg cgccgccttg acggttccaa
    14101 ccgccagcgc tccgccattc agcatggata tcagtgccga aatggacggg acactatccg
    14161 gcccacccat ccccaattgc gaccgcatcc ccgatacgcg tgcggatgcc gacgaggttg
    14221 ccgccaatgc gtccgcctcc cccgtcttgc cagagtgcga accacccgcc tggatctggg
    14281 ccagcaatgc cttggccagg ccgatgcccg ggttggccag ttgcagcgcg agctgttcgt
    14341 cggccatacc ctgcgccatt tttgtctgct cgctgtcgaa cgggccagag agcgtggacg
    14401 cttggcgcgc ctgcttgagg aactgctgga tgaacaacgc ctcgaactga tgcgccacct
    14461 ttttctgctc ggcctcgggt gcgttggcac ccgccgccac accctgcttc aggctgtcca
    14521 gattgctgaa atcgaagatg gacgacgatg cggcctcggg catgtgaatg tagcggacca
    14581 gcgacatggg cgaaactcct cagatgattt ccagatctgc gtgcagggcg ccggcgctct
    14641 tgagcgtctg cagaatggac aacagatcct gcggcgtcgc gcccagcgcg ttgagcgcgc
    14701 gcaccacgtc ggccagattg gcgctcgtgc gcatatgctt gatggtgcca ttgcttgtgc
    14761 tcattgcgat ctgcgtgttg ttggtgacga cggtctgacc cccggcaaac ggtgtgttgg
    14821 gctggctgac ctgctgctga ttgctgatca ccacggacag attgccgtag gtgaccgcgg
    14881 cctcatcgat ggtgaccgtg ctgttcatga ccaccgagcc ggtgcgcgca ttgatgatga
    14941 ccttggcgcg cacgggcggc atccgcacct gcagattctc cacttgcgac agaaagcccg
    15001 cctgcgccga cgggtccagc ggcccgcgca ggcggatgac ccggccatcc accgcgctcg
    15061 ccgtttccgg ccccatggcg cgattcaacg cggccaccac gttgttcacg acgccgaagt
    15121 cagtgttgtt caattccagc cggatgaccc cgtcgtgggc gtatgttgtc gggacgtcac
    15181 gctcgacgat ggccccgccc ggaatggtcc cgccgttgag ctgattgacc tgaaccttgg
    15241 acccgcctga ttgcgcgccg gcgccgccca ccagcaggtt cccctgggcc atggcataga
    15301 cctggccgtt ggcccccttg agcggcgtca tcagcaatgt tccaccgcgc agactcttgg
    15361 cattgcccat ggacgacacc acaacgtcca gggtctgtcc aggccgggaa aacgccggga
    15421 gcctggctgt caccatgacg gcggcgacat tcttgacctg catgttggtg ccctgcggca
    15481 ctgtcacacc caattgcgac agcatgttcg tcagactctg ctgggtgaat ggagtctggc
    15541 gcacctggtc gccgctgcca tccagcccca ccaccaaccc gtaccccacc agttgattgg
    15601 cacgcacgcc ttgtatcgag gcgatgtcct tgatccgctc ggcatgcgcc gcgggcaacg
    15661 ccaacaaccc cagggccaga gcaacggcca ctcggcgtgc ctggcatcgc agtgatgaaa
    15721 aacagcggac agtacggaac agactcatca tcgggacaca ggctcagaac ggagaaatat
    15781 tcaggaagaa gcgttgcagc caacccatgg tctggacctc gtccatcgtg cccttgctgc
    15841 ggtactccat gcgcgcgtcg gcgacctggg tcgacaacac cgtgttcgac cccgtgatgg
    15901 agcggggatc gacgacaccc gagaaccgga cgtactcact gccacggttg atggcgatct
    15961 gcttctggcc ggcgatctgc aggttgccgt tgggcagcac tccgacaacg gtcgtggtga
    16021 tggtgccaga gaacgtgttg gcggcgctgc tcgacccctt tccttccatc ttgttgccac
    16081 cggacgcatt catgtcctgc ttggaattga gggaattcgg gaggatcgcg gccggcatca
    16141 catccatgcc cagcgacgcc gagctcgtgc ggttggtggc cgtatccacg ttcttggcgg
    16201 cgttggtctt ttcctgaatg atgatggtga cgatatcccc cacattgcgc gggcgccgat
    16261 cttcgaacaa cgggtagtcg ccatacgccg tgggctggta gatcgatccg ttcggcaccg
    16321 gcggcggcag acgcggtggt ggcgcggcgg tgagcgggcc ggtgacgatc ggctctggcg
    16381 gcaccagcgc gcagcccgac acgaccgcca gcgcggcgca cgccgccatg gcgcggaatc
    16441 ctttctgcat gttcgtcgcc acgccgcctt acatctgtgt cagccgcgcc agcatctggt
    16501 cggatgtctg aatggccttg ctgttcattt cgtaggcgcg ctgcgtggtg atcatgttca
    16561 ccagttcctc ggccacgttc acgttggagg tttccacata tttctgcagc acgagaccgg
    16621 cgccatccag gccgggctgc tcgtaattgg ctgggcccga cgcgtcggtt tcggcgtaca
    16681 gattctcgcc ctcgctctga agacccgtgg ggttgacgaa ggtggccaat tgaagctgac
    16741 ccacgttcac gttggttcca acggctcctg gctgcgtcac cgacaccgtg ccatcgcgcg
    16801 cgatggtcag gctcagcgcg ttgtcgggaa tgttgatggg gggctggatg ggatatcccc
    16861 ccgatgtcac caattggccg ttctggtcga cttgcaggct accgtcgcgc gtgtagcgga
    16921 aggtgccgtc cggcatctgg acctgcagga acccccggcc ctgtatggcg atgtccatgg
    16981 ggttgtcggt ttccttcaga ttgccctggg tgtggatgcg ctcggtggcg acggtgcgca
    17041 caccggtgcc gatctgcagg ccggagggaa gctgattgct gtccccgacc tgggcgcccg
    17101 gttgccgcag ggtctggtac atcaggtcct ggaacacggc gcgcgattgc ttgaagccat
    17161 cggtgttgac gttggccaga ttgttggaaa tcacatccat gttggtctgc tgcgcttcca
    17221 ggcccgtctt ggcgatccat aacgaccgta tcattgtcca ttcctttttt gaggcgccgc
    17281 aatgcggcgc gaaatccggt tttgccgcgg gccaatcact tggcgggcgc caggatcgaa
    17341 ttgccctggt cctcgttcgc gccggcatcc gagatgacct tcatctgcat gtcgaagcgg
    17401 cgggaattgc tgatcatgcc gaccatggcc tgcgccgggc tgacattgct ctgctcgacg
    17461 aagcccgaaa cgatgcgcac attggggtcg gcctgagcag gcaccccacc ccccggacca
    17521 acgcgaaaca gaccatccgc cccttgcgac agctggctcg ttggtggatc gaccagcttc
    17581 aagcgcccga tctgctggac ttcggtgggg ttgtcgccag cgcccagcac atcgacggca
    17641 ccgtctccgg tgaaagtcac acgccctctt tccggcacct gtatgggtcc gccgttatca
    17701 cccaggaccg gcagaccacc ctgcgccgtc accagttggc cttgcgcatt cacggcgaac
    17761 gacccacccc gggtatacgc ttcgccgctg ggggtctgca cgctgaacca gccctgcccg
    17821 gtgatggcca cgtccagggg acggccggtt tcgcgcatgg cgccctgctg aaaatcagaa
    17881 cccggcgtgg aggtcacagt gccgacgcgc gtgggcagcg tgtcggggct ctcgaccggc
    17941 accgatcggt agatcgccag ttggcgccga aagccatcgg tgctggcgtt cgccaggttg
    18001 ttgctgatga cggcctgttg ctccaacgtg cgctgggcgc cgcccatggc ggtgtagatg
    18061 atccgatcca tggtgaacct agatgcccga gatcagggtc tgcatcacct gatcctgcgt
    18121 cttgatggtt tgcgcattag cctggtacgt gcgttgcgcg atgatcatct taaccagctc
    18181 ctggctcatg tcgacgttcg attcctcgac cgcctgcccc ttcacagagg ccagaccatt
    18241 gctcccaggc cgtcccagaa taggctggcc tgagtcggcg gtttgtgccc aggcattacc
    18301 gccgaccggt ttgagtcctt gcagattgtt gaagttcgcc agcgccagcg tgcccagcgt
    18361 ctgggtttca ccattggtgt agttggctac gatggaaccg tctccggcga tggacatact
    18421 agcgtattcc ccactgtagt aaccattctg atgaaaattc gcggcaaaat cacttccgaa
    18481 ctgtgtggac cccgagtaat ccatcgcgac agacatgggt gcggcgggag acccggcccc
    18541 gcctggcgcc gcaatagtca cggtggccgt gggcggcgaa gtgacaatgg cgccagcgct
    18601 attgaaggtc agtttctgcg ccccatccgc aggcgaaccg accaccgccc cgtccaatcg
    18661 gtagttcaca tcccacacgc tgttcgtccc ggagggtgcc cgcttggcga aatactgcgt
    18721 gagctgatgc gcattgccga gcgagtcata caccgtcatg gagcgctgat aggtatagct
    18781 gctggaattc gtgatatcga atggcgtgac ggcaggatcg atgacagcgg cgttggcatc
    18841 gaggttcgtg gtgaacgagg ccgtggacgt cgcggttggc gccacgttcc cgaccggcac
    18901 tttgacgggc gccagattgt tgcccgtcgt gtcgtagccc atcaggcgct ggccttgaga
    18961 gttaacgatg tagttgtcct tgtcggcatg gaactggccg ttgcgactgt agaacgtgtt
    19021 gccgctaggg tcctgtacca cgaacaaccc gttggcgcca tcgatcgcca tgtcgaactg
    19081 gttgccggtg ttggtgacgg tgcccgccga gaaattctgg ttgacgcttg ccacctgcac
    19141 ccctaggccc acacttgagt ttgcgaacac gtctgcgaac gtggccgccc ccgacttgaa
    19201 gcccaccgtg ccggaattgg cgatattgtt accaataaca tctagatcct gtgacgccgc
    19261 gttcaaaccg ctcagccctt ggccaaaacc catgattcac ctctttagac gttgtttcat
    19321 caaaatcgag caaccaggcc ctccgtgcgc cacgatgcgt ctcgacggaa agcggctgac
    19381 gccattcagg acaggatctg ccggatgtcg gacaatgacg cgctgcccgc caaccccaga
    19441 tcaatcttcg gaccgctgga cgaatactgc acactggaca ccttgccgta tgtcagggcg
    19501 tcggccgaca cgggcccgcc ttggccatcg agcgcagtaa ccttgatgtt gtaagcgcca
    19561 tctggcactg gcgcgccgcc gtcccccatg ccatcccacg acaccgcctg cactccggcg
    19621 ggcagcgcgc ccagatcgat ctgacgcacc gccttgccgc tgctatccat gatggtgacc
    19681 accaccttgg ctgcgggaga gatgacatcc acgccaaaag gcgtagtctg cttgaccgtg
    19741 gggttggtcg ggtcgctccc tagcgatacc ttggtgcccg gaacgagtac acctttcccg
    19801 ataaggccgg ctgcctgcat ggattgcgac acatccattt gacccgacag agcaagcaag
    19861 gtgttgttga gttggttgat accgttgacc gtggatagct gggcgatttg agacgtcacc
    19921 tcggcgttat ccatcggatt gagcggatcc tgattctgca actgcgtgac gagcagcgtc
    19981 agaaactgac cctgcgtatc ggacatcaga ctattcgtgg aagcgctggc agccgccagc
    20041 gccgccgtgc cgttggaggc actggtggct ggaaccccgt tcgtgctggt catgaaggat
    20101 tttctcctga aggattctga ttggctgcgg gcatttcccg gcgcttattg ccctatggtc
    20161 aaagcctggg ccatcaggct cttggcggtg ttgatgacat ccacgttggc ctgatacgac
    20221 cgggacgccg aaatcatgtt cacggtttcg gccaccacgt ccacattggg catggtgacg
    20281 tagccgcgct cgtcggccag gggattgccg gggtcgtact tgagcttggg cggcgcggag
    20341 ctttccacca caccggctac gcgcacgccg ccaatcgcgg cgggaccgga ttggtcggca
    20401 tgcggttgcg tctggaaaat cacctcgcgt gcccgatagg gctggccgtc cggtcccacc
    20461 gcgctgtcgg cgttggccag gttgctggcc gatacgttca tgcgctggga ttgggcctcg
    20521 agcgcggacc cggcgatact gagaatgctc atcatgattg aattccccca gccttattgc
    20581 tgcaacgcca gcagcatcgt cttgatgcgc tgggaaacca gatcgagatc cgcctggtag
    20641 tgcatggtgt tctcggcgaa cgccacgcgc tctgtgttca tgtcgaccgt gttgccgtca
    20701 agactgggct gcgtcggcac gcgataaagc agatccacag gccccgacga ggtggcttgg
    20761 ccaggaatgt gacgcggcga ggtgagggtc agatgcgtat ccggcaaccg catgtcatca
    20821 cccaacgcat tctgaagggc tttgtcgaaa tcgaagtcgc gcgccttgta gttcggcgtg
    20881 tccgagttcg cgatgttcga cgccagcact tcctgccgcc gctgccgcaa ggcgatcgcc
    20941 gcctgataga aactcaaatc gctgccaatg gggtcggcca tgactcgctc tctgtggact
    21001 gggtttacgt ctgccgccaa ccgcca
//
LOCUS       DASTNK010000125        40874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1393764, whole genome shotgun sequence.
ACCESSION   DASTNK010000125 DASTNK010000000
VERSION     DASTNK010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 40874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40874
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393764"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /gene="gatB"
                     /locus_tag="VFR20_09110"
     CDS             <1..889
                     /gene="gatB"
                     /locus_tag="VFR20_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU0230720.1"
                     /translation="NMQEGSFRMDANVSVRPRGQQEFGTRSEIKNLNSFRFLERAIQY
                     EVRRQIELIEDGGKVVQETRLYDSDRDETRSMRSKEDAHDYRYFPDPDLPPLVIADDW
                     VQRVRAAMPELPAQKRARFEHDLGLSGYDAAQITMDRGTADFFEQTAAGVPAGQAKIA
                     ANWVLGELSALLNRNDIDIARSPIHPATLAALINRILDGTISNKIARDVFAAMSEGEQ
                     RGDPDAIIEARGLRQISDSGAIEAMIDEVLAAHPAIVEEYRGGKQKAFNSLVGQIMKA
                     ARGKANPQQVNDLLKKKLD"
     gene            complement(999..1673)
                     /gene="pyrE"
                     /locus_tag="VFR20_09115"
     CDS             complement(999..1673)
                     /gene="pyrE"
                     /locus_tag="VFR20_09115"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_033448256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU0230721.1"
                     /translation="MTDPATSRTDFVRFSLDQGVLRFGQFKVKSGRVSPYFFNAGLFD
                     SGRSVGRLAGFYAQALVDSGIEFDMLFGPAYKGIPLSTATSIALANHPAMKGHDVPFA
                     FNRKEAKDHGEGGVLVGAPLKGRVVIIDDVITAGTSVRESVGIIRQAGATPAAVLIAL
                     DRMERAGADHELSTHSAVQDVSQSYDMPVVSIASLDDIMALLEDDASLAAHREAVAAY
                     RAKYGV"
     gene            1914..2330
                     /locus_tag="VFR20_09120"
     CDS             1914..2330
                     /locus_tag="VFR20_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU0230722.1"
                     /translation="MKSDCCATWAPRMLSVLRIVSGYLFLLHGATKLFVMHVPLASLY
                     GVAGVIEFIGGILILLGLFGRVAAFICSGEMAFAYFMGHVAAKGHVLTPINNGGDAAV
                     LFCFVFLYIAAAGPGPWSIDHLRHKARHSQRPGHLI"
     gene            2340..3170
                     /locus_tag="VFR20_09125"
     CDS             2340..3170
                     /locus_tag="VFR20_09125"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEU0230723.1"
                     /translation="MTLPVYFISHGGGPWPYMPEAARGNAALAASLRDIPRQIGRRPS
                     AILMVSAHWEAAAFTVATASHPGMVYDYHGFPENTYHVNYPAPGSPDLARRVFDCLNA
                     ARLPAALDDERGFDHGAFVPAHVMYPEADVPMVQLSLHAGYDPREHLALGRALAPLRN
                     EDVLIVGSGLSYHNLRLMGSAGALPSARFDEWLQQVVVDGPVEERTAQLEQWASAPSA
                     RLAHPREDHLIPLMVAVGAAESEIAVCIYHETTAFGGITASSFRFGSTGSAISQPSGH
                     "
     gene            3193..3981
                     /locus_tag="VFR20_09130"
     CDS             3193..3981
                     /locus_tag="VFR20_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019249085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU0230724.1"
                     /translation="MVRITSLNANGIRSAFRKGLQPWLSGYHPDIVCLQEIKIHADDL
                     TDELSNPPGYQGHFCHAEKKGYSGVGLYLTQPAQTVATGMGCPEFDAEGRIIRADYPK
                     FSVISAYLPSGTSGDERQQAKYRFLDHFGGWLGQLMKECKKTGREFVLCGDWNIAHKE
                     IDLKNWRGNQKNSGFLPEERAWLTEVFDKQGFIDVFRTLNPEPDQYTWWSNRGQAWAK
                     NVGWRIDYHVATPGIAAKAVGASIYKDERFSDHAPLTIDYDTRL"
     gene            complement(4045..5493)
                     /locus_tag="VFR20_09135"
     CDS             complement(4045..5493)
                     /locus_tag="VFR20_09135"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU0230725.1"
                     /translation="MKSRTDHPDTEAFDDAWSSAPFEAGLVTQDFRPEPSAVIKHELT
                     GAGLRDDLEALRDSERRMALAIAGSNTGIWDRDVLNDRIHYSAGWKALLGYAENEIGD
                     QLTESYTRVHPDDLSYVRASMQAHFDQKTPVYEVEHRLRCKDGSYKWVSSRGQVVQRD
                     GNGQALRMIGTTTDITAMRDMSDRLRQTAELLTSLTDEVPGLVFQYQRLPDGKTCFPY
                     ASAGIQDIYELEPSQVMTDACLLDALIHPDDLAAYHASLDDSARDLTPWHLEYRVVLP
                     RQGLRWRQGVARPRRQADGRVLWHGFITDVTERHTMEDELRELALLDGLTKLPNRRCF
                     MQQIDAELERIKRENGKPAAVLMCDLDHFKGINDRWGHVTGDVALQHFSGILQTHLRK
                     SDVAGRVGGEEFAIMLRATDLAEASGFARRLQGRMRQTPLEQGGHVIPITLSIGIAIM
                     GTADPDAMSVLSRSDAALYEAKAKGRDCIELR"
     gene            complement(5683..6933)
                     /locus_tag="VFR20_09140"
     CDS             complement(5683..6933)
                     /locus_tag="VFR20_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide transporter"
                     /protein_id="HEU0230726.1"
                     /translation="MIGSSANVYASPRVTPLLVLGFASGLPLALTSGTLQAWATVSKV
                     SLQDIGFLTLVGSAYTLKFLWAPLVDRYAPPLFGRRRGWMFCTQILLALGIAAMGVMS
                     PAHDLGALAALAVLVAFLSATQDIAFNAYSTDVLRKHELAAGAALQVLGYRLAMIVSG
                     GLALVLAGEWLGWSNTYFLMGALMALCAIASWLAPEPEERCAEPKTLAIAVVEPLAEF
                     FRRPGAWTILALIVLYKLGDAFAGALSTTFLLRGTGFSLAEVGTVNKIFGLAATIIGA
                     LVGGSLMARLGLYRSLMLFGIFQAISNFGYWVLAVTPPHLYSMAVVVAVENLCGGLGT
                     AAFVALLMALCNHEFSATQFALLSALSAVGRTYLAGPLTPPLVEHLGWPTFFMFTVFI
                     GVPGLVLLWWRRKEIRGLESLGVA"
     gene            complement(6934..7572)
                     /gene="metW"
                     /locus_tag="VFR20_09145"
     CDS             complement(6934..7572)
                     /gene="metW"
                     /locus_tag="VFR20_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEU0230727.1"
                     /translation="MIETPLAGPGHALRLDLVRIAGWIASGSRVLDLGCGDGALLAHL
                     RDEKRVTGAGVELDDDHVIASVRRGVSVIQQNLEEGLALFDDQQFDTVVLSQTLQSMH
                     RTEHILREMARVARFGIVSFPNFGYWPHGWSILRGRMPVTGEMPYQWYDTPNIHLCTL
                     RDFERLAVSLGLRITDLATFNTHKEVSFLPGWRSTLAVYRFESNQHPSPSQE"
     gene            complement(7569..8846)
                     /locus_tag="VFR20_09150"
     CDS             complement(7569..8846)
                     /locus_tag="VFR20_09150"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEU0230728.1"
                     /translation="MVPSSAFAADSSPSTSTAFASPHHGSPIDEASVPPGSVGLVSPE
                     IIRFDEPLALASGQVLPSYELAVETYGTLNAAGTNAVLVCHALNASHHVAGMSAHDPK
                     DIGWWDNMVGPGKAVDTTRFFVIGVNNIGSCFGSTGPSSVNPATGEPWGAAFPVLTVE
                     DWVRAQARLADRLGIHKFAAVMGGSLGGMQALSWAISCPERVEHCIVIASTPRLSAQN
                     IGFNEVARRAIISDQDFHGGDYYAHGVVPTRGLSVARMVGHITYQSNEVMAEKFGRTQ
                     RAPAADGGFHYGYGIEFEVESYLRHQGEKFSRYFDANTYLLLTRALDYFDPARACGGN
                     LARALAQVQAGFLLVSFATDWRFPPSCSREIVRALLKNQRPVTYAEIDAPHGHDAFLL
                     GDQRYHAVVQGYYDRIAQGLNLPERHLTTGVLA"
     regulatory      complement(8836..8914)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            9085..9246
                     /locus_tag="VFR20_09155"
                     /pseudo
     CDS             9085..9246
                     /locus_tag="VFR20_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(9284..9793)
                     /locus_tag="VFR20_09160"
     CDS             complement(9284..9793)
                     /locus_tag="VFR20_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230729.1"
                     /translation="MWGRLLLALALSVPLAGCSPDYNWRTVSLAGGEVSSILPDKPIA
                     KSRTLDFAGHNVTLTFLLAEVNGTLFAVGVAPLPDALRADAPLRTEMGRQVIESFYHG
                     LKLPVPKVLPGFGKPFALKGPDTNKQTELHAAVWVTPQALVEAMVTADAARFPAQEAT
                     RFLDSTKVR"
     gene            9922..11487
                     /gene="gshA"
                     /locus_tag="VFR20_09165"
     CDS             9922..11487
                     /gene="gshA"
                     /locus_tag="VFR20_09165"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEU0230730.1"
                     /translation="MTSSTSSLASLRDQPSLFKNILRGIEKEGLRVDGKGFLAATPHP
                     RAFGSALANPHITTDYSEALLEMITGTHNGVEGLLEELHDTHRFVAANLAGEMVWNQS
                     MPAHLPDEADIPIAWYGTSNTGMLKHVYRRGLAERYGKTMQCIAGLHYNFSVPDTLWD
                     LLGITGASAEDQRSKGYLSLIRNFNRYSWLLMYLFGCSPAVSVRFLRGMPGHTLSRLD
                     DDTLYLPHATSLRMSDLGYKNETQSELQLCYNDIETFLTRLYDAVTTPWPQYQAIGTH
                     RDGEWIQLNTNVLQIENEYYSSIRPKRATGRCERPVTALAARGVQYVEVRCLDIDPFE
                     AVGISAQTCHFMDTFLLFCAAEESPFFPNGGICTTSHNNFGLVAKEGRRPGLTLINED
                     TPVALSDWGRDILDRMRPYAELLDHALGENGHAAAVEAQRPKIHDTEQTPSARLLRLL
                     RDEGVSMHDFSLRQSRRHHEALLATPISASRAKVFEAAAARSVEEQARLERGDTEDFA
                     AYVARYHAALKRP"
     gene            complement(11489..12022)
                     /locus_tag="VFR20_09170"
     CDS             complement(11489..12022)
                     /locus_tag="VFR20_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0230731.1"
                     /translation="MRSCEQVVLLADYNAWMNKRTYEAAGRLSSSDLAVDRKAFFGSI
                     LGTLNHLVVGDTLWLKRFATHPAGYPALDPIRALPMPVSLDALLFTDFPALEEHRKML
                     DDAVCDWAGSVTDADLEQTLRYKNTKGIESNRLFFSLVLHFFNHQTHHRGQVSTLLFQ
                     AGVDVGVTDLLALIPQV"
     gene            12118..13551
                     /locus_tag="VFR20_09175"
     CDS             12118..13551
                     /locus_tag="VFR20_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEU0230732.1"
                     /translation="MNSNEHTAILAVDLGSTRLKAAWVGPDGRLGPVAHTESPIRSSA
                     RRAEAIWKGVTALIGDLLRSPEAPDRILAVALTGVTRSHVFLDKDGSVLGPVVFWDDP
                     YGEDRATEVAAAYRSPDDTTGYGAFHPLARLAQFRLDCGKAPAAMVELKDWLNFRLTG
                     RLATDSVAHGRIQSGMANAPSATQVLAQLEFPASVIPPPMAPHARLGTVLTLDDRRLR
                     KLAGVPVATSSFDSWCSTLGMGAVVDGGVYDISGTTEVMGLLGSEPRTVPGMVCMPWT
                     PALWHTGGPCQTGLGTLAWFARAFLDRDDPAATLEAAQAAPSQDPPVCLPYPSGERMP
                     WWNANLSASFHSVRTHHTRSDLARAVVEGLALAHRLALDKAGAFDAGTIIRMGGGGTS
                     LPHWCQTRADAFGVPIALGANPESALVGAALAAGVAVGYHSDLSATQDAVNQHNVLIH
                     PDPGRMRYFDSRAATFAMLLDQTLAST"
     gene            13601..14557
                     /locus_tag="VFR20_09180"
     CDS             13601..14557
                     /locus_tag="VFR20_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0230733.1"
                     /translation="MTGATPRLWLERYGTCAAGAILFLFFALFAPNFLALQNLANIVE
                     RISFMAILGMGFALALLVAELDLSFANICSLAAVVVGWLVHTGHAPAVAVLAGLAVGA
                     GAGVLNGLLVTVLRVPSLIATLATASIANGFAFMITQGVALVGQWDPRFLWIGRGTVF
                     GIPALIFWLIVVGVATLFLLKQTRLGFRMLAAGEASEATRLAGVNVRRIKIIGLALSG
                     LAAGITAVLMTSNLASASPDSAGDLMLTSIAAVLLGMTMFEPGRPNVPGTLIGAIIIG
                     MLGNGLVLLGVQYYVQDILLGVIIVASVAFSSSVLKKAAFSV"
     gene            14671..15624
                     /locus_tag="VFR20_09185"
     CDS             14671..15624
                     /locus_tag="VFR20_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU0230734.1"
                     /translation="MTTFLRRLAAAMILLLGFAGAAQSAPTLDIIGFQMSSETHARVV
                     NAAAAAARAQGWNVQILNSKGDMPTHIQQLQTAIQSKPTGIIVAMGKPLQAEEQFKAA
                     KAAGIPVVTVMSGASPYVLFDIAVNEYSAGAKAALFLMDHLNYRGNILTERYEGNTGT
                     RIRGKILDVVLSENPAVKVIGSHTMARAASWRNDVKAGMSALLLKNQGKVDGIWASFD
                     GQAYIIDDLLQQQGAKKGHPILVSIDGGAESYRRIADPKSLMMATVAIPFEKMGIKAF
                     DIIDRVAIKKESKESIHHGPYIYMDAELVDAANVAQYLKKP"
     gene            15628..16377
                     /locus_tag="VFR20_09190"
     CDS             15628..16377
                     /locus_tag="VFR20_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0230735.1"
                     /translation="MTPVLELHGLAKRYGAVDALKGVDLTVHEGEVLAICGDNGAGKS
                     TLIKVVAGAHPRSAGAMRLSGRDVQWRSPHDALVNGVATIYQDLALAARLSIWQNIFI
                     GSELRRRIAPGCLLLDKKAMRTQAAGYLRQLNQTIDDVDRPVSDFSGGQRQAVAIARA
                     LRWKAKLVIMDEPTAALGVKETAEVLALIGQLKTQGVTVLLISHNMEDVVRVADRVVI
                     LKAGRAVAGGPTAGVSPTSLAQAIITGCWPG"
     gene            complement(16611..17891)
                     /locus_tag="VFR20_09195"
     CDS             complement(16611..17891)
                     /locus_tag="VFR20_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0230736.1"
                     /translation="MFATYAARWLGRRNIHYAWMMALLVFLTMLVSAAAIGLPGAFIR
                     PLSHEFGWSVEQISSALALRFALFGLMGPFAAILMERYELRTLMCGALVLITVALLSA
                     TMMTQVWQLVVLWGLVLGLGAGLIALVLGAVVANRWFDKRRGLVIGLLTASSSTGQLA
                     FLPLAAWLIEHVGWRVALLPVGASCLVVAGLVYCMVCSHPEDVSSVPYGAEPGAKPLM
                     ARTASRPSFMEPLTTLRAVSRNRTFWVLFGTFFICGLSTSGLIQTHFISLCGDYGLAE
                     LPAASVLAMMGVFDVVGTIASGWLSDRLDNRKLLFWYYGLRGLSLFWLPHSEFTFYGL
                     SLFAMFYGMDWIATVPPTVKLTSATFGRLRAGLVFGWIFTGHQIGGAIAAYGAGLVRT
                     LLLTYNPALYAAGGACLVAAVAVFLIRRPAAAAT"
     gene            complement(18017..18721)
                     /locus_tag="VFR20_09200"
     CDS             complement(18017..18721)
                     /locus_tag="VFR20_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0230737.1"
                     /translation="MLKVDDLHVAYGGVQAVRGISLEVRHGEITALLGANGAGKSSTL
                     LSIVGSVKPKAGRVTFDGDDITALTPDALVRRGIALIPEGGRVFARQPVEQNLRLGAY
                     TVRKGAVYQERLDQVYALFPRLAERRAQIAGTLSGGERQMLAIGRALMSGPRLLLVDE
                     PSLGLSPLLVETVFDTLADLNRSGTSILLVEQNMAQALEIAARAYVMQSGAIALQGNA
                     EEILSSDNVRQAYLGL"
     gene            complement(18726..19451)
                     /locus_tag="VFR20_09205"
     CDS             complement(18726..19451)
                     /locus_tag="VFR20_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0230738.1"
                     /translation="MALLEVENLTKRFGGLIANRDINLSVEAGEIVAIIGPNGAGKST
                     LFNGLAGHHAPTSGHVRFDGKELVGRTPEAIAAMGLMRTYQIPRSFAAMTVLENVMVG
                     ALLRHPTLDAAREVAWRVLDSVGLTDRGMTPAGELNVAGQKRIELARALASSPKMLLL
                     DEVAGGLNPSEAVALAEILRSIHAGGVTLLIVEHVLEVVMRLAHRVLVLDFGQLIASG
                     TPEDVVRNPTVIEAYLGRKYRAG"
     gene            complement(19432..20514)
                     /locus_tag="VFR20_09210"
     CDS             complement(19432..20514)
                     /locus_tag="VFR20_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0230739.1"
                     /translation="MGEPNQLSPQRQEGAAPPAGSAPPPAAGIASPAAGVMPDPRAPA
                     RRRTALIWLVIVIVALAIFPFLVPDPFSQQIVVMLLMYGALGAAWNLVGGFLGRVSFG
                     HAVFLGVGAYTTLLLLQNWHLSPWLGIPLGGVVAAAVAFIVGRPTLRLTGHYFAMATI
                     ALLSVAQLLVVNWSWAGGATGVEAPIANNAWLLLFRSKAPYYWIAFGLALLTLWATVA
                     LARSKTGYYWRAINGDEAAARSLGVPAERYKMWAFVLSAAITGVWGGFYAIYIGFIDP
                     DSAFSLTLSIQIVLVAILGGIGSLVGPWLGAAVLIPLGEGMRVALGSSGSGLDLLLYG
                     LAIILVSLFLPKGLITLRFRRGSSGS"
     gene            complement(20516..21367)
                     /locus_tag="VFR20_09215"
     CDS             complement(20516..21367)
                     /locus_tag="VFR20_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0230740.1"
                     /translation="MFEALVTGLLNGLIYALIAVGLALIWGITDVINFAHGDFLMLGM
                     YAAYWLFTLGHVDPTLSAPLVACLLGFVGFLSFALIIRPLQRGPPMIVILATFGLGLV
                     LRQLAFIAFSPDYRNLPNTWLSGSVSVGGINLGLPQVGTGIVSLVVIVLLFFLVYRTR
                     WGHALQAVAEDRQAASLVGIASDRVNAQVWILGSAAVGLAGALLTTFFYVFPSVGNVF
                     GLLAFVAVAMGGFGSLPGAFISGILIGVIESMTGYLFLPAYKMVSIFLIFLLVLWYRP
                     RGLFGRW"
     gene            complement(21447..22697)
                     /locus_tag="VFR20_09220"
     CDS             complement(21447..22697)
                     /locus_tag="VFR20_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0230741.1"
                     /translation="MKKLLAVLLSCLGIALAQNATAQDKVITIGAIYPLSGALASTGT
                     QLKQAIELAVDIVNNKHPELKGIPLAAGAGLPKLDGAKIKVIFADSQGSPSVGQAEAQ
                     RLIDHDHVVALVGAYQSAVTKTTSRIAEQRQIPYMNPESSSPELTERGYKWFFRTTPN
                     DETFIHNMMQFLDGIKNLPTKKIAVVYENTDFGVNTYKAVEKFAKQSGRTIVANIAYS
                     AGAPSLNAETQKLAAAKPDVAIFASYTSDAMLFVRTMRQMKYAPPIFLANDAGFIDPR
                     FISEVGPQVQGVLTRDVWGNDLYTAKPIIKQINDMFKAKTGNNLNGNSARSVQGMLVL
                     ADAINRAGSTKPDAIRQALAATNLTVNQIIMPWEGVKFDKTGQNVEGQGLILQLEGTE
                     YHTVWPARYKKMADLPKLPFTWGK"
     gene            complement(22881..23654)
                     /locus_tag="VFR20_09225"
     CDS             complement(22881..23654)
                     /locus_tag="VFR20_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HEU0230742.1"
                     /translation="MTPSDLRHQIRQGHFRKPTAGVCGDYAQANLAILPREQAADFMR
                     FCHLNPKPCPLLGVGEPGQWQMPMLGDDIDIRTDLPGYYVYRNGERAEELTDLREIWQ
                     PGFVVFAIGCSFSFEHMLMQAGVPLRHIQEGRNIAMYRTSVPNGKAGPFGGNLVVSMR
                     PMTSRNAIRAIQITSRFPAVHGAPVHLGDPSLLGIADLQAPDYGDAVEIHADELPVFW
                     ACGVTPQEAIKSARLPLAITHKPGHMLVTDLLNSALAAF"
     gene            23844..24782
                     /locus_tag="VFR20_09230"
     CDS             23844..24782
                     /locus_tag="VFR20_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0230743.1"
                     /translation="MNTRFIETFIMLARLKSFRATANALHATPAAISQRIKSLEDDLR
                     TELIDRTTKTFRLTANGNRLLGHARSIMDAVRKMEAAAHQEGTVRGCLRLGVNETVVH
                     SWLAQYMTLLNRTYPELEVELSVEISSVMQKLLLAGDLDLVLRVEGIDNEKIVSDALA
                     IYPVRWIARKGFVPQRKGDAIKTMLRYPVLTFGRGTAPQRAIVQIVSTLANQADIPVG
                     QARVMCSPSVAAIVQLIRDGFGVAAIPALFVANEIDSGAFVELPIQPTPPSIVLSMCR
                     HADADRYVHAAADVARAACGEYCRQTDRRLVEAVAD"
     gene            25020..26702
                     /gene="ggt"
                     /locus_tag="VFR20_09235"
     CDS             25020..26702
                     /gene="ggt"
                     /locus_tag="VFR20_09235"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU0230744.1"
                     /translation="MRPATAASRSPAHGTHGMVVTAQHLATQVGLNILKAGGNAIDAA
                     VATGYALAVVDPCCGNIGGGGFMTLHLADGRNIFINFRERAPLRATRDMYLDKQGKVV
                     PGLSTQGYLAVGVPGTVMGLDTALRKYGTMTRKQVMAPAIRLARNGFVLEQGDIDILT
                     GKGKHGDLDALTGPSKRFATQPNVAAIFLNHGQAYRVGDRLVQKDLARTLALIEKKGS
                     SAFYDGPIADAIVHASQANGGILGKVDFTNYRAEEARPIECGYHGYQVISAPPPSSGG
                     TTLCEILNIIEPYPLDKWGFHSAAATHVMVEAMRHAYVDRNTLLGDPHFVKAPIARLL
                     SKAYAARIRTAILPDKATPSSEVKQGVAPHEGTNTTHYSIVDDKGNAVSVTYTINRYF
                     GAGVIAGKTGFFLNDEMDDFTSKVGVPNLYGLIQGENNAIAPGKRPLSSMTPTIVTKN
                     GRLFMVVGSPGGSRIITITLETFLNVVDHGMTLQEAVDAPRFHHQWQPDVIQYEPDAF
                     TPEVMASLKAMGYKFKAYGPWGAAEAILVNPATGALTGAADKRQAAGAAMGY"
     gene            complement(26821..27435)
                     /locus_tag="VFR20_09240"
     CDS             complement(26821..27435)
                     /locus_tag="VFR20_09240"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU0230745.1"
                     /translation="MPEGSGHSSADVAGILLAAGIGRRYDPSGRAFKLLAPVLPGRNL
                     LQVSAGKLLAATDELLIVHGPRSLQSYPVVAELPARQFSCLQAGQGMGRSLQSAISCS
                     SPRLGWLVALADMPYISRATYESLRQALMLGALLARPVYRGQPGHPVALSVSLRPDLM
                     ALRPEEGAGPLFKTYRTRAVQIDVDDAGCVADVDTPEALARFNA"
     gene            28288..28611
                     /locus_tag="VFR20_09245"
     CDS             28288..28611
                     /locus_tag="VFR20_09245"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HEU0230746.1"
                     /translation="MNPFPVRLPMLHAPEIQEKSRNARLEARVSSDQKDFFQRAANLT
                     GRSLSELVIDSTQEAAAKIVQEHEAIRLSREEQVAFVSALLTPSEPGPRLQKAVQSYR
                     QKAGF"
     gene            28612..29130
                     /locus_tag="VFR20_09250"
     CDS             28612..29130
                     /locus_tag="VFR20_09250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0230747.1"
                     /translation="MALILAPEHINPLTKSHQRKSFDCGHENLNRYLREQARQDAEKR
                     VAAPFVLTQPGDPKVLGFYTLSSSIIPVDELPPDLMKRLPRYGQLPVTLLGRLAVDHS
                     AGGQGVGEFLLIDALRRSLEAAQQIAAMAVIVDAKNERAESFYRHFDFQPFQQTPLRL
                     FLPMAQIAKLFA"
     gene            complement(29333..29668)
                     /locus_tag="VFR20_09255"
     CDS             complement(29333..29668)
                     /locus_tag="VFR20_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230748.1"
                     /translation="MGDVFDQYDLPVFQTLDAFFVGHNYGRDGRVGDSVKELFYLFCD
                     LRQFGLERLGAGCACCKAMIPQVLEHSPCRLQEISRGLHLFQQVLELPFDNVAADGLS
                     VTIAAARVT"
     gene            complement(29895..29969)
                     /locus_tag="VFR20_09260"
     tRNA            complement(29895..29969)
                     /locus_tag="VFR20_09260"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29934..29936),aa:Arg,seq:ccg)
     gene            30286..31239
                     /locus_tag="VFR20_09265"
     CDS             30286..31239
                     /locus_tag="VFR20_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU0230749.1"
                     /translation="MTSTEEHKESHESFIKTPRQLIVTIILAFLIPIIIIIMLVHMVV
                     VSTPAGAGSDADSTKTILARIQPVASLKLVDANAPKTYLTGKQVFDSTCTACHTAGVA
                     GAPKVGNKADWAPFIKTGYQEMIKNAIHGVGAMPPKGGNASLSDYEVARAIAYMANQS
                     GASFKEPPPPAPASAKKVAAAAVPAPVVAATAPAAAAPAQPAAEPAKAAAQPAAEPAK
                     AAPAPIKVAAIPGLAPDAATNPEGEKLYKTVCFACHATGVMGAPTFGSMEKWGPFIKT
                     GIDTMVKNAIHGVGNMPPRGGSQATDAQMRAGVEYMVSHAK"
     gene            complement(31368..32909)
                     /locus_tag="VFR20_09270"
     CDS             complement(31368..32909)
                     /locus_tag="VFR20_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEU0230750.1"
                     /translation="MKTIHTIGIVGAGAMGRGIAQIAAQAGRNVLLFDMNAQAVEAAR
                     GHLEAMWGKLVAKGRIRGETAAQSLARVTACADLAALAKADLVVEAVVERLDVKQELF
                     SRLEGIVAEDCVLASNTSSLSITAIAAACRLPQRVAGYHFFNPVPLMRVVEVIDGLRT
                     DPAVGDTLMALARELGHTPVRARDMPGFIVNHAGRGMNTEGVRLAQEAVAPFHHVDAI
                     MREQAGFRMGPFELLDLTALDVSHPVMESIYRQFYDEPRFRPSPVTAVRFAGGMLGRK
                     SGEGFYRYENGTKLVPPEPAPPALPDGLKVWLAPFHSVGHKRAATLLKSLGMQVLTGS
                     APPPDALILVTPFGEDTATVAQAHQLDATRTVALDTFFGLEQGRRRVLMCSPATEPRW
                     RDAAHALMASDGTAVSVINDSPGFVGQRMVATIINVASDIAQQKIASPRDIDLAVSLG
                     LGYPGGGPLSLGDGLGSAHVLEVLRAMRHVTGDARYRPSLWLQRRVQLGLSLGAEPLC
                     DAMSE"
     gene            33154..33387
                     /locus_tag="VFR20_09275"
     CDS             33154..33387
                     /locus_tag="VFR20_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230751.1"
                     /translation="MSVNAYDQYVKLSDSLERELTREALSCGEPVSIVDVAKSIAKGV
                     KITVRAIARVLSDATRPLGNAHSHDNQLSSARW"
     gene            complement(33391..35565)
                     /locus_tag="VFR20_09280"
     CDS             complement(33391..35565)
                     /locus_tag="VFR20_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU0230752.1"
                     /translation="MAFDPVNGHTVPVDLNSRQREAVMYLDGPCLVLAGAGSGKTRVI
                     TQKMAYLLRECGYQGRDIVALTFTNKAAREMGERVRKLVDARLGKGLTIGTFHSLGLR
                     FLREEARHAGLKPQFSILDAHDALAIIQELLATTDKARLRAVQQTISLWKNAQLDPDG
                     AERAAATPSEAEAARVYRSYSATLAAYQAVDFDDLIRLPAMLLESNPEVRERWQRRVK
                     YLLVDEYQDTNLCQYHLVRALTGMRSLFTAVGDDDQAIYAWRGATVENLAKLTTDYPA
                     LKVIKLEQNYRSVQRILTAANQVIGHNPNLFGKKLWSDLGQGEPIQVTPMDDDLAEAE
                     SVAIRISAARFERRAQWRDFAVLYRSNQQSRVLEQALRNLRIPYTISGGQSFFEKPEV
                     RDILAYLRLIANEDDDPAFIRAVTTPRRGVGQATLQALGQLAAEKHLSLFAALYEIDE
                     ARVPARQLASLRVFGELIQNFQRRAGRSNVQVFAREPDLFTTDGASSPPGEAQSVVGK
                     TVPGHRSEAEPAPALLDELLGLIQYERHLFDTLDERAAQGRWQNVLELVGWLKRKAQE
                     DGLDLIELTQHVALVTLLQRGDDEEPDAVKLSTLHASKGLEYPHVYLVGVEEGLLPHT
                     GRDEEDADPAQAAEALAQRIQEERRLMYVGITRARRSLHLSWCRKRRRARDDVVREVS
                     RFISEMGLEEAGMTEDPALRALSPSQRLGMLKDLLDAPRKKD"
     gene            complement(35571..37673)
                     /locus_tag="VFR20_09285"
     CDS             complement(35571..37673)
                     /locus_tag="VFR20_09285"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU0230753.1"
                     /translation="MTHLTSPSDNVLAALPADTVWVRVALDVPLDGLFDYRAPDTASV
                     GQRVIVPFGRRKLMGVVVSLPEKPAVDAGLIRDVDQILDDLPPLPPDWMRLATFAAQY
                     YQRPLGEVMLPALPAGLRKVSAYQGKRSAGGPVCRIDRRRAPGAKEIEADQAPALNAE
                     QALAADMIESVRGHKTVLLHGVTGSGKTEVYLHAAQDMLARGRQVLFLVPEINLTPQL
                     ERSLRARLAPLAGADGLAVLHSGLSEGERLRAWLSASRGQARILLGTRLSIFTPMPEL
                     GLIIVDEEHDASYKQQEGLRYSARDLAVWRGHDAGLAVVLGSATPALESWHHARRGHY
                     LLATLPHRAHPVDPPRVRLVDTRRLVLEQGFSPQLIDALAQRLEAGQQSLVFLNRRGY
                     APVLNCHSCGWVSQCPRCTAYAVLHRRDARRHYLQCHHCGHQMPAPRACPDCGDQDLQ
                     AMGRGTQRVEERLADLFPSARVARIDADSTRLKGSAEKLFARIHAGEIDIMVGTQMVS
                     KGHDFENLGLVGVLNADAMLFAQDFRAPERLFAQLMQVAGRAGRHARGAEVIIQTGYP
                     EQPVYQALTRHDYVGFAQYSLAEREAVGLPPYAYQALLTAEAPELAIAVDFLARAKAL
                     AGTLPSSDVDTAAVTLYDPVPLRVVRVANVERAQLLVESRHRPALQGFLASWSRTLPA
                     LAAAARARCQLEVDPLEI"
     gene            complement(37994..39088)
                     /gene="hemE"
                     /locus_tag="VFR20_09290"
     CDS             complement(37994..39088)
                     /gene="hemE"
                     /locus_tag="VFR20_09290"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEU0230754.1"
                     /translation="MSVSPPVLTNDIFLRALLREPVPHTPIWLMRQAGRYLPEYNQTR
                     ARAGSFLGLAQNPEYACEVTLQPINRFGLDAAILFSDILTVPHAMGLGLGFTEGEGPR
                     FASPVRTEADVARLTVPDMGDLRYVFDAVALIRRELAGKVPLIGFAGSPWTVGCYMVE
                     GGSSGDYRIIKSLLYRRPDLLHRMLDVNARAACEYLNAQIQAGAQAVMIFDSWGGVLA
                     DGLYQEFSLAYTRRVVQGLIKTHEGRTVPSIVFTKGGGPWLEDIAGCGCDAVGLDWTV
                     NLARARARTGDKVALQGNLDPMVLFGDESSIRTQARRVLDDFGPVGQGGHVFNLGHGI
                     SRFTPPEAVGTLVDEVHTHSRAFHADGALK"
     gene            complement(39196..39957)
                     /locus_tag="VFR20_09295"
     CDS             complement(39196..39957)
                     /locus_tag="VFR20_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230755.1"
                     /translation="MSTSCTHSGISHWFDGRHTPGRLRLQALKLEVDVAQPGSSQLLA
                     SSEMALRRFDACLITVSECNLAWVRTSLLAARGRVHTPIIALAHQLKAAALDDLYSLG
                     LADFVRGPVCTEELRTRVERRLDSPKYRLLPGAPAGGLGECAPDYGASPHTGVTEDAL
                     CASILDRSGLELDAFAAATASRSATSRESFKSAKSKVIERFERAYITAALGRHSGNIA
                     MAARSAQKHRRAFWALMRKHQIEAAPYRAESQTDA"
     gene            complement(40292..40714)
                     /locus_tag="VFR20_09300"
     CDS             complement(40292..40714)
                     /locus_tag="VFR20_09300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU0230756.1"
                     /translation="MANLHVDVVSAEESIFSGEAKFVSLPGEAGELGVMPGHTPLISR
                     IRPGTVKIVLAEGGEESIFVAGGILEIQPGVVTVLSDTAIRAADLDEAKAEEARKRAE
                     ETLRNATEKEDIAAVEAELAMLAAQVAAARKLNQLRRH"
     gene            complement(40731..>40874)
                     /locus_tag="VFR20_09305"
     CDS             complement(40731..>40874)
                     /locus_tag="VFR20_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU0230757.1"
                     /translation="SPGKYVPLAETLRGFKMIVNGECDSLPEQAFYMVGTIDEAIEKA
                     KKL"
BASE COUNT         8195 a        13308 c        12390 g         6981 t
ORIGIN      
        1 caacatgcag gaagggtcgt tccgcatgga cgccaacgtc tcggtgcgcc ccaggggcca
       61 gcaggaattc ggcacgcgca gcgaaatcaa gaacctgaac tcgttccgct ttctcgagcg
      121 cgccatccag tacgaggtcc gtcgccagat cgaactcatt gaagacggcg gcaaggtggt
      181 gcaggaaacc cggctgtacg attcagaccg ggacgagacc cgcagcatgc gcagcaagga
      241 agatgcgcac gattaccgtt acttccccga tcccgacctg ccgccactgg tcatcgcgga
      301 cgactgggtg caacgggtgc gggccgccat gcccgaacta cccgcccaga aacgcgcccg
      361 attcgaacat gacctcgggc tgtcaggcta cgacgcggca cagatcacca tggaccgcgg
      421 caccgccgac ttcttcgaac agacggcggc cggcgtgccg gctggccagg ccaagatcgc
      481 ggccaactgg gtgctgggcg aactgtcagc cctgctcaac cgcaacgaca tcgacatcgc
      541 tcgcagccct atccacccgg ccacgctggc tgcgctgatc aaccgcatcc tcgacggcac
      601 catctcgaac aagatcgcgc gcgatgtgtt cgcggccatg agcgaggggg aacagagggg
      661 tgacccggac gccatcatcg aagcacgcgg cctgaggcag atcagcgaca gcggggccat
      721 agaggccatg atcgacgaag tcctcgccgc ccatcccgcc atcgttgaag agtaccgtgg
      781 cggcaagcag aaggcgttca actcgctggt cggtcagatc atgaaagcgg cccgcggcaa
      841 ggccaaccca cagcaagtga acgatctgct aaaaaagaaa ctggactgat cccgcttgcc
      901 tgatttgcgc ccattcggca gcactggatc gggccgatcc gcctcccgtc cggcccgccc
      961 tccagggcga accgggcacg gccccgttcc gcaagaggtc agacgccgta tttggcgcgg
     1021 tacgcggcca cggcttcgcg gtgcgcggcc agactggcgt cgtcttcgag cagcgccatg
     1081 atgtcgtcga gagaagcgat gctgaccacc ggcatgtcgt aggactgcga cacatcctgc
     1141 acggcggaat gggtggacaa ctcgtgatcg gcccccgcgc gctccatgcg gtcgagagcg
     1201 atgagcaccg ccgcgggcgt ggcgccggcc tggcggatga tgcccaccga ttcgcgcacc
     1261 gacgtgcccg cggtgatcac atcgtcgatg atgaccactc gccccttgag cggcgcccca
     1321 accagcacgc cgccctcgcc atggtccttg gcctctttgc gattgaaggc gaacggcacg
     1381 tcgtgcccct tcatggcggg atgattggcc agcgcgatgg acgtggcggt cgacaacgga
     1441 atgcccttgt aggcggggcc gaacagcatg tcgaattcga tgccggaatc aaccagggcc
     1501 tgcgcataga aacccgccaa ccggcccacc gaacgcccgg agtcgaaaag ccccgcattg
     1561 aagaaataag ggctcacccg ccccgatttg accttgaact gcccaaaacg caatacaccc
     1621 tgatcgagag aaaaacggac gaaatcggta cgggaagtgg cgggatcagt catggcgcga
     1681 caagtcgggg ggcggataat ggcgaggctc acattatcgt tcatatttca aaccgcaccc
     1741 aaccgtacta tataaacggg cgggcgtggc ccaagttgtc atcccgcgaa cgattcctat
     1801 acactgtacg cgcgccaatc ggcgtgcggc aatccgggcc acccggcctg atcgggcttg
     1861 gcggttcttg ttcactcctg caagccacca aatagaaaga ggaaaggtcc accatgaaaa
     1921 gcgattgttg cgccacttgg gcgccgcgca tgctaagcgt gctgcggatc gtcagcggtt
     1981 atctgttcct tctgcacggg gcgaccaagc tgttcgtgat gcacgtacca ctggcctcgc
     2041 tctacggcgt tgccggcgtc atcgaattca ttggcggcat cctcatcctg cttggcctgt
     2101 tcggtcgggt cgcggccttc atctgctcgg gcgagatggc gttcgcgtac ttcatgggcc
     2161 acgtggcggc taaaggccac gtgctcaccc ccatcaacaa cggcggcgac gcggccgtgc
     2221 tgttctgctt cgtgttcttg tacatcgcgg cggcgggtcc agggccatgg agcatcgacc
     2281 atctgcgcca caaggcccga cacagccagc gtcctggtca tcttatctag agccacatca
     2341 tgacgttgcc cgtgtacttc atctcccacg gcggcggccc ctggccctac atgcccgagg
     2401 ccgcgcgcgg caatgccgcg ctggcggctt cgctgcgcga cattcccagg cagatcggcc
     2461 gacgcccttc ggccattctc atggtttcgg cccactggga agcggcggca ttcacagtgg
     2521 ccaccgcatc gcacccggga atggtgtacg actaccacgg ttttcccgaa aacacctacc
     2581 acgtcaatta tccggcgccc gggtcgccgg atttggcccg gcgcgtgttc gactgcctga
     2641 acgcggcccg gctacccgcc gcgctggatg acgaacgggg cttcgatcac ggcgccttcg
     2701 tcccggccca cgtcatgtac ccggaagccg acgtgcccat ggtgcaactg tcgctgcatg
     2761 ccggctacga tccgcgcgag catctggcgc tgggcagggc attggcgccg ctacgcaacg
     2821 aagacgtgct catcgtcggc agcggcctga gctaccacaa tctgcgcctg atgggatcgg
     2881 ccggcgccct gccgtcggcc cgattcgacg agtggctgca gcaagtcgtc gtcgacggcc
     2941 ccgtcgaaga acgcaccgcg cagctcgaac aatgggcgtc ggcaccatcg gcgcgcctgg
     3001 cgcacccgcg tgaagaccac ctcatccccc tgatggtcgc ggttggcgcg gccgagtccg
     3061 agatcgcggt ctgcatctac catgaaacca cggcctttgg cggcatcacg gcatccagct
     3121 tccgctttgg ctcaacgggt tcggccatca gccagccgtc agggcactga cttcctcttt
     3181 caaggatccg ctttggtacg catcacctct ctcaacgcca acggcattcg ctccgcgttt
     3241 cgcaagggcc tgcaaccttg gctgtccggc taccaccccg acatcgtctg cctgcaggaa
     3301 atcaagatcc acgccgacga tctgactgac gagttgagca acccgcctgg ctatcaaggg
     3361 cacttctgcc atgccgaaaa aaagggctac agcggcgtgg gactgtacct gacacagccc
     3421 gcgcaaaccg tggccaccgg catggggtgc cccgagttcg atgctgaagg ccgcatcatc
     3481 cgggccgact atcccaagtt cagcgtcatc agcgcgtatc tgccgtccgg caccagcggc
     3541 gacgaacggc aacaagccaa ataccgattc ctggaccatt tcggaggctg gctcggtcaa
     3601 ctcatgaaag aatgcaaaaa gaccgggcgc gaattcgttc tgtgcggcga ctggaacatc
     3661 gcccacaagg aaatcgacct gaagaactgg cgcggcaacc agaagaactc cgggtttctg
     3721 cccgaagaac gcgcctggct caccgaggtt ttcgacaaac agggttttat cgatgtgttc
     3781 cgcacgctca accctgagcc cgaccaatac acatggtgga gcaatcgggg ccaggcctgg
     3841 gccaagaacg tggggtggcg catcgactat catgtcgcca cacccggcat cgccgccaaa
     3901 gccgtcggcg cgtccatcta taaagacgag cgcttcagcg accatgcacc gttgacgatc
     3961 gactacgaca cgcgcctgta gcgaaaccag gcgagggggc ctcgcatgga gcccctcgcc
     4021 gtgttgggcc catcgcgtgg aatctcaacg gagctcgata cagtcgcggc cctttgcctt
     4081 ggcctcatac aaggcagcgt cgcttcggga aagcaccgac atcgcatccg ggtccgcagt
     4141 acccatgatg gcgattccga tgctcagcgt gatggggatg acatggccgc cctgctcgag
     4201 tggtgtctgc ctcatcctgc cttgcaggcg ccgtgcgaag cccgacgcct cggcgagatc
     4261 ggttgcccgc agcatgatcg cgaactcctc accgccgacg cgacctgcga catcgctctt
     4321 tcgcaagtgc gtctgcaaga ttccggaaaa atgctggagc gccacgtcac ccgtcacgtg
     4381 cccccaacga tcgttgatgc ccttgaagtg gtccagatca cacatcagca ctgccgccgg
     4441 tttgccgttc tcgcgcttga ttcgctcaag ttcggcatcg atctgttgca tgaaacagcg
     4501 tcgattgggc agcttcgtca gaccgtcgag cagcgccaat tcacgtaatt cgtcttccat
     4561 ggtgtgacgc tcggtcacgt ctgtgatgaa gccatgccac aggacgcggc cgtccgcttg
     4621 gcgtctgggg cgggcgaccc cctgccgcca acgcagcccc tgtcgcggca ggacgactcg
     4681 atattccaag tgccagggcg tcagatcgcg cgcggaatca tcaagcgagg cgtgataggc
     4741 ggccaaatcg tcggggtgga taagagcgtc caacagacag gcatccgtca tcacctgtga
     4801 cggttcgagt tcatagatgt cctggatgcc cgcactcgcg tatgggaaac aggtcttgcc
     4861 atcgggcaat cgttgatact gaaacaccag cccagggact tcgtccgtca ggctggtgag
     4921 cagctccgcg gtttgccgca accgatcgga catatcacgc atggcggtga tgtcggtcgt
     4981 ggtgccgatc atgcgcaacg cctggccgtt accgtcgcgc tgcaccacct ggccacggct
     5041 cgatacccac ttgtaagagc catccttgca gcgcaggcga tgttccactt cgtaaacagg
     5101 tgtcttctgg tcaaaatgcg cttgcatcga cgcacgcaca tacgacagat catccgggtg
     5161 gacacgagta tagctttcgg tcagttggtc accgatctcg ttctccgcat agccaagcaa
     5221 tgccttccag ccagcggaat aatggatcct gtcgttgagg acgtcacgat cccagatacc
     5281 tgtgttactg ccagcgatag ccaacgccat cctgcgttca ctgtcgcgca gcgcctccag
     5341 gtcgtcccgc agtcccgccc cggtcagttc atgcttgata acggcggagg gttccgggcg
     5401 gaaatcctgt gtgaccaacc cggcctcgaa cggcgcgctc gaccaggcgt cgtcgaaagc
     5461 ctctgtgtcc gggtggtcgg tgcgggattt cattgtcggg ggctcaagaa tcgaatccga
     5521 cgctggccca accacgggca ggccttgacg caagaatcgt ctgccttcgg aatcgaaact
     5581 atccggaaac tgtgatcatc aacattttac aatatgttgc gcaatattta taccattccg
     5641 cagcgcgatg atcgggattg gcccacggcg gggaatgctg tgtcaggcga cgccgaggct
     5701 ctccaggcca cggatctcct tgcggcgcca ccatagcaac accaaaccag gcactccgat
     5761 gaaaaccgtg aacatgaaaa acgtcggcca cccaagatgt tcgaccagcg gaggggtaag
     5821 tggcccggcc aggtaggtgc gcccgaccgc cgacaacgca gagagcaacg cgaactgcgt
     5881 ggcggaaaac tcgtggttgc acagcgccat cagcaaggcc acgaacgccg ccgtgcccag
     5941 cccaccgcat aggttctcca ctgcgacaac caccgccatg gagtacaggt gtggcggtgt
     6001 caccgccagc acccaatagc cgaaattgga tatcgcctgg aaaataccga acaacatcag
     6061 cgaccggtac aaacccagac gcgccatgag ggatccaccc accagcgccc cgatgatggt
     6121 cgcggccagg ccgaagatct tgttcaccgt gcccacttcg gccaggctga agcccgtgcc
     6181 gcgcagcaag aacgtggtgg acagcgcacc cgcgaacgca tcgcccagct tatacaggac
     6241 gatcagggcg aggatggtcc acgcccccgg ccgccggaag aactccgcca gaggctcgac
     6301 caccgcgatg gccagcgtct tgggttcggc gcagcgctct tctggctcag gcgcaagcca
     6361 cgaggcaatg gcgcacaaag ccatcagagc ccccatcagg aaataggtat tgctccaacc
     6421 cagccactcg cccgccagga ccaatgccag cccgcctgag acgatcatag ccagccggta
     6481 acccagaacc tgcaacgccg cgcccgcggc aagctcatgt ttgcgcagga cgtcggtgct
     6541 gtatgcgttg aacgcgatat cctgggtcgc cgacaaaaac gcgaccaata cggccagcgc
     6601 ggccagcgcg cccaaatcat gcgccggcga catcaccccc atggccgcga tacccagagc
     6661 cagcaggatc tgcgtgcaga acatccagcc gcggcggcga ccgaacagtg gtggcgcgta
     6721 acggtccacc agcggcgccc acagaaattt cagcgtgtag gcggaaccaa cgagagtcag
     6781 aaaaccgatg tcctgcaaag acaccttgga caccgtggcc caggcctgca acgtgccgct
     6841 cgtcaatgcc agcggcagcc cgctggcgaa tcccagcacc agcaacggtg tgacgcgggg
     6901 gctggcataa acgttggcgg atgacccgat gatctactcc tgggatggcg acggatgctg
     6961 gttcgattcg aaccggtaaa ccgcaagcgt actacgccac cccggcagga aggaaacttc
     7021 cttgtgcgta ttgaacgtgg ccaggtcggt gatgcgcagc cccagcgaca ccgccagacg
     7081 ctcgaaatcc ctgagtgtgc acaggtggat attgggtgtg tcgtaccact gatacggcat
     7141 ttcgccggtg accggcatac ggccccgcag gatggaccag ccatgcggcc agtagccgaa
     7201 gttggggaac gacacgatgc cgaaacgcgc gacacgcgcc atttcccgca gtatgtgctc
     7261 ggtacgatgc atggattgca gggtttgcga cagcaccact gtgtcgaact gctgatcgtc
     7321 gaacagtgcc agcccctctt ccaggttctg ctggatcacc gacacacccc ggcgcactga
     7381 cgcgatgacg tggtcgtcgt cgagttccac ccccgcgccg gtcacgcgtt tttcgtcacg
     7441 caggtgggcg agcaatgcgc catcgccaca gcccaggtcg aggacacggc tacccgatgc
     7501 gatccagccg gcgatgcgga caagatccag gcgcagggca tgacccggcc cggcgagtgg
     7561 cgtctcgatc atgccagaac ccccgtcgtc aagtggcgtt ccggcagatt caacccctgt
     7621 gcgatgcggt cgtagtaccc ctggaccact gcatggtagc gttgatcgcc caacaggaat
     7681 gcgtcgtggc catgcggcgc gtcgatctcg gcatacgtga cgggccgctg atttttcagc
     7741 aaggcgcgca cgatctcgcg cgaacacgac ggagggaagc gccagtcggt ggcgaacgac
     7801 accagcagga aacctgcctg gacctgggcg agcgcgcggg ccaggtttcc gccgcaagcg
     7861 cgcgccggat cgaagtaatc cagcgcccgg gtcaacagca gatacgtgtt ggcatcgaaa
     7921 tagcgcgaga atttctcgcc ctgatggcgc agatacgact cgacttcgaa ttcgatgcca
     7981 taaccgtaat gaaagccgcc gtcggccgcc ggcgcccgct gcgtgcgacc gaatttctcg
     8041 gccatgacct cgttggactg atacgtgatg tgtccgacca tgcgcgccac cgacaggccc
     8101 cgagtgggca ccacaccgtg cgcgtaatag tcgccgccat ggaaatcctg gtcggagatg
     8161 atggcccgcc gcgcaacctc gttgaagcca atgttctggg ccgacagccg cggcgtgctg
     8221 gcaatgacaa tgcaatgctc gacgcgttcc gggcaggaaa tggcccagct caatgcctgc
     8281 atgccgccca gcgacccgcc catcacggcg gcgaacttgt gtatacccag ccggtcggcc
     8341 aggcgggcct gcgcccgcac ccagtcttcg accgtgagga cgggaaacgc ggccccccag
     8401 ggctcgccgg tggcgggatt gacgctggac ggtccggtgg agccaaagca cgagccaatg
     8461 ttgttgaccc cgatgacaaa aaagcgggtg gtgtcgaccg ccttgccggg ccccaccatg
     8521 ttgtcccacc agccgatatc cttggggtcg tgcgccgaca tgcccgccac atgatgggag
     8581 gcattcagcg cgtggcacac cagcaccgca ttggtgccgg ccgcattgag cgtgccatag
     8641 gtttcgacgg ccagctcgta gctgggcaac acctggccgc tggctaaagc caggggttca
     8701 tcaaagcgga tgatttccgg ggaaacgagt ccgaccgagc cgggtggaac cgacgcttca
     8761 tcaataggcg agccatggtg aggcgaagcg aacgcggtgg acgttgatgg ggaagaatcg
     8821 gcggcgaaag cggaagacgg gaccatacgg acctctttag ctggatttat caggcgcccg
     8881 caagcagatc aagcaaatcg gcgccactat cggaaggctt cattctagca ccatcgatac
     8941 ggaaatcgat gacgaatcca ccagccggca cactggaaat cacagacttg tccgggccga
     9001 ttttactgaa atgaccaaaa ttgataaatt ccatcaattt ttatcatcat caaaaaaaca
     9061 cgaccacctg gggatccaca aacaatgaag ccgcttaccg gaagggaaat cacctgtctg
     9121 cactgggcat ccatgggcaa gaccagttgg gagatgggga tgatcctggg tctatccgaa
     9181 cgcaccgtca atttccatat catcaacgcg tgcaagaaac tcaacgtgcg cggacgccag
     9241 gcagccatca cctccgcctt gtgcgccggc ttgttgcccg tggctaccga acctttgtcg
     9301 aatccaggaa tcgcgtggcc tcctgagccg gaaaccttgc ggcgtcggcc gtcaccatgg
     9361 cctccaccaa tgcctgtggc gttacccata ccgccgcatg cagttccgtc tgcttattcg
     9421 tatccggccc cttcagcgcg aaaggcttgc caaatccagg aagcaccttc ggcacaggca
     9481 gcttcagccc gtgataaaaa gattcgatga cttgcctgcc catttcggtg cgcaaggggg
     9541 catcggcccg caaggcgtcg ggcaatgggg cgacccccac ggcgaaaagc gtgccattga
     9601 cctcggccag gaggaaagtc agggtgacat tgtgccccgc gaaatcgagc gtgcgggact
     9661 tggcgatggg cttgtccggc agtatcgacg acacctcgcc tccggccagt gaaaccgtgc
     9721 gccagttgta gtcgggcgag catcccgcca acggcactga caacgccagc gccaacaaca
     9781 gcctgcccca catgacgccc tccgtgtttt gttcgcgtta cgataccact ccgaccacgg
     9841 ggacgcaccg aggcttcatt ctcctatcga gtagaatttc cagccaggat tcgacagacc
     9901 ccataaggga acgaccgcgc aatgacatca agcacatcca gcctcgcatc gctacgcgac
     9961 cagccgtcgc tgttcaagaa cattttgcgc ggcatagaaa aagaaggcct gcgcgtcgac
    10021 ggcaagggct tcttggccgc cacgccacat ccccgcgcgt tcggctcggc cctcgccaac
    10081 ccgcacatca cgaccgacta ttccgaagcg ctgctggaaa tgatcaccgg cacgcacaac
    10141 ggcgtggaag gcttgctgga ggaactgcac gacacccatc ggttcgtggc cgcgaatctt
    10201 gccggggaaa tggtctggaa ccagtccatg ccggcccacc tgcccgacga ggccgacatc
    10261 cccatcgcct ggtatggcac gtccaacacc ggcatgctca agcacgtgta tcggcgcggg
    10321 ctggccgaac gctacggcaa gacgatgcaa tgtatcgccg gcctgcacta caacttttcg
    10381 gtgccggaca cgctgtggga cctgctcgga ataaccggcg cgtcggccga ggaccagcga
    10441 tccaagggct acctgtcatt gatacgcaac ttcaatcgct attcgtggtt gctcatgtac
    10501 ctgttcggct gctcgccggc ggtctctgtg cgttttctgc gtggcatgcc agggcatacg
    10561 ctgtcccggc tcgacgacga cacgttatat ctgcctcatg ccaccagcct gcgcatgagc
    10621 gatctgggct acaagaatga gacccagtcc gaactgcagc tttgctacaa cgacatcgaa
    10681 acgttcctga cgcggctgta cgatgccgtc accacgcctt ggccgcagta tcaggccatc
    10741 ggcacgcacc gcgacggcga gtggatacag ctcaacacca acgtgctgca gatcgagaac
    10801 gaatactatt ccagcatccg ccccaagcgc gccacgggcc gctgcgagcg cccggtcaca
    10861 gcgctggcgg cgcgcggtgt gcaatacgtc gaggtgcgat gcctggatat cgatccattc
    10921 gaagccgtgg gcatttcggc tcagacctgc catttcatgg acacgttcct gctcttctgc
    10981 gcggccgagg aaagcccgtt cttccccaac ggggggatct gcacgaccag tcacaacaat
    11041 ttcggacttg tggcgaagga aggacgccgg cctggcctga cgctcatcaa cgaagacacc
    11101 ccggtagccc tgtcggactg gggccgggat atcctcgacc gcatgcggcc ctacgccgaa
    11161 ttgctcgatc acgccctggg cgagaacggc cacgcggccg ccgtcgaggc gcagcgcccc
    11221 aagatccacg acacggagca gacgccgtcc gcccgtctcc tgcggctact gcgcgatgaa
    11281 ggcgtcagca tgcatgactt ttcgttgcgg caaagccgtc ggcaccatga agcgctgctg
    11341 gcgacgccca tctcggcgtc ccgggcgaaa gtcttcgaag ccgccgccgc ccgctccgtt
    11401 gaagaacaag cccgcctcga acgcggagac accgaggact tcgcggccta cgtcgcgcgc
    11461 tatcacgccg cgcttaaacg gccgtgactc acacctgggg aatgagcgcc agcagatcgg
    11521 tcaccccgac gtccacgccc gcctggaaca acagcgtcga aacctggccg cgatgatgcg
    11581 tctggtggtt gaagaaatgc aggacaaggc tgaagaacaa tcggttcgat tcgatacctt
    11641 tggtgttctt gtagcgcaga gtctgctcca gatccgcatc ggtcacagat cccgcccagt
    11701 cgcatacggc atcgtccaac attttgcgat gctcctccag cgccgggaaa tccgtgaaca
    11761 acaaggcgtc gagcgatact ggcatgggca gggcacggat cggatcgagc gcgggatagc
    11821 ccgccggatg tgtggcaaag cgcttcaacc acaaggtgtc gccgacgacc agatgattga
    11881 gtgtgccaag aatggatccg aaaaacgcct ttctgtcgac ggccagatct gacgaagata
    11941 gtcggcccgc ggcctcatag gtgcgcttgt tcatccaggc gttgtaatcc gccaggagca
    12001 caacctgctc acacgatctc atgactcgct ccacaagaga cgatgccccc catggtaacg
    12061 cgactgcgga ccgcgccggt tacacttcga cagcggaaac ccaccccttc actcaccatg
    12121 aacagcaacg aacacacagc cattcttgcc gtcgacctgg gttcgacacg gctgaaagcg
    12181 gcgtgggtcg ggcccgacgg tcgattgggc ccggtcgcgc acaccgaatc gcccattcgc
    12241 tcgtccgcgc ggcgggccga agccatctgg aagggcgtca ccgccctcat cggcgacctc
    12301 ctgcgatcgc ccgaggcgcc agaccgcatc ctggccgtgg cgttgactgg cgtcacgcgg
    12361 tcgcacgtat ttctggacaa ggatggcagt gtgctgggcc cggtggtgtt ttgggatgat
    12421 ccgtacggcg aagaccgggc caccgaagtg gccgccgcct acaggtcgcc cgatgacacc
    12481 accggctatg gcgcgttcca ccctcttgcc cgattggcgc aattccggct cgactgcggc
    12541 aaggcccccg ccgccatggt cgagttgaag gactggttga acttccggct gactggccgc
    12601 ctggcgacgg attcggtggc gcacgggcgc atccagtcgg gcatggccaa cgctccgagc
    12661 gcaacccagg ttctcgccca gctggaattc ccggcatcgg tcatcccccc gccaatggcg
    12721 ccgcatgcca gactgggcac ggtgctgact ctcgatgacc gtcggctccg aaagctggcc
    12781 ggcgtacccg tcgcgaccag cagtttcgac agctggtgct ccacactggg catgggggcg
    12841 gtggtcgacg gcggcgtcta tgacatctcg ggaaccaccg aggtcatggg cctgctcggt
    12901 tcagaacccc gcaccgtgcc aggaatggtc tgcatgccct ggacaccggc gctttggcac
    12961 accggggggc cgtgccagac agggctgggg acgcttgcct ggttcgcccg cgccttcctc
    13021 gacagggatg atcccgcagc cacgcttgaa gccgcccagg ccgcgccgtc gcaggacccg
    13081 cccgtctgcc tcccttatcc atccggagaa cgcatgccgt ggtggaacgc caatttgtcc
    13141 gccagctttc actcagtgcg gacgcatcac acccgcagcg acctcgcccg cgccgtcgtc
    13201 gaagggcttg ccctggcgca tcgtctggcc ctggacaaag cgggcgcttt cgacgcaggc
    13261 acgatcatcc gcatgggcgg tggcggcaca tcccttccgc actggtgcca gacccgggct
    13321 gatgcgttcg gcgttcccat cgctctgggc gcgaacccgg aatcggcgct ggtgggcgcg
    13381 gcgctggccg caggcgtcgc cgttgggtat cattccgatc tgtcggctac gcaagacgcc
    13441 gtcaaccagc ataacgtcct gatacacccc gatccgggcc gtatgcgcta ctttgattcc
    13501 agagcggcga ctttcgcgat gcttctggac cagacgctcg cctcgacatg acctgttcca
    13561 agaccgactc atcagccacc ccggattggg ccattcaagc atgaccggcg ccaccccacg
    13621 cctatggctc gaacgctatg gaacctgcgc ggccggtgcc atcctgttcc tgttcttcgc
    13681 gctgttcgcc cccaacttcc tggccctgca gaacctcgcc aacatcgtcg aacgcatcag
    13741 cttcatggcc atactgggca tgggtttcgc gctggccctg ctggtggctg aactcgatct
    13801 gtcgttcgcc aatatctgca gcctggccgc cgtggtggtg ggttggctgg tgcacaccgg
    13861 ccacgcaccg gccgtcgcgg tgctggccgg cctggccgtg ggcgctggcg cgggggtgtt
    13921 gaatggcctg ctggtcaccg tcctgcgcgt gccatcgctc atcgccacgc tggccacggc
    13981 atccatcgcc aacggcttcg ccttcatgat cacgcagggc gtggccctgg tcggacagtg
    14041 ggatccgcgt ttcctctgga tcgggcgtgg cacggtgttc ggcattccgg ccctgatttt
    14101 ctggctgata gtggtcggtg tagccacgct tttcctgctg aagcagacgc ggctgggctt
    14161 ccgcatgctg gccgcgggcg aggccagtga ggcgacgcgg ttggccgggg tcaacgtgcg
    14221 ccgcatcaag atcatcggcc tggcgctgtc ggggctggcg gccggcatca ccgccgtgct
    14281 catgacgtcc aacctggcct cggcctcgcc cgattccgcc ggcgacctga tgctgacatc
    14341 catcgccgcc gtgctgctgg gcatgaccat gttcgaaccg gggcgcccca acgtgccggg
    14401 caccctgatc ggcgccatca tcatcggcat gctgggcaac ggcctggtgc tgctgggggt
    14461 gcagtactac gtgcaggaca ttctgctggg cgtgatcatc gtggcgtccg tcgcgttttc
    14521 atcgtccgtg ctcaagaagg ccgctttttc cgtatgacgc acattattcc cttataaacc
    14581 caactctttc ccacatcagc ctcatccctc tacaagtcag ttcaaacata tcattcgaac
    14641 aacgccacca atttcgccaa ggggaacacc atgaccactt tcctgcgccg cctcgccgcg
    14701 gcgatgatcc tgctgctcgg cttcgccggg gcggcgcaaa gcgcgccgac cctggacatc
    14761 atcggcttcc agatgtcgtc cgagacgcac gcccgggtgg tcaacgcggc ggccgccgcc
    14821 gcgcgggccc agggttggaa cgtgcagata ttgaacagca agggcgacat gcccacccac
    14881 atccagcaat tgcagaccgc gatacagtcc aaacccaccg gcatcatcgt cgccatgggc
    14941 aagccgctcc aggccgagga gcagttcaag gcggccaagg cggcgggcat cccggtcgtc
    15001 acggtcatga gcggcgcgag cccctatgtg ttgttcgaca tcgccgtcaa tgaatactcg
    15061 gccggcgcca aggccgcgct gttcctcatg gaccacctga actaccgtgg caacatcctc
    15121 acggaacggt atgaaggcaa taccggcacc cgcatccgcg gcaagatcct cgatgtcgtg
    15181 ctcagcgaaa acccggccgt gaaggtcatc ggttcgcaca ccatggcccg cgccgcgtcc
    15241 tggcgcaacg acgtcaaagc cggcatgagc gcgttgctgc tcaaaaacca gggcaaggtc
    15301 gacggcatct gggcctcgtt cgatggccag gcctacatca tcgacgacct gctgcaacag
    15361 caaggcgcga agaagggcca tcccatcctg gtcagcatcg atggcggcgc cgaatcctac
    15421 cggcgtatcg ccgatccgaa atccctgatg atggcgaccg tggccattcc gtttgaaaaa
    15481 atgggcatca aagcgttcga catcatcgat cgcgtcgcca tcaagaagga atccaaggaa
    15541 agcatccacc acggtccgta catctacatg gatgccgagc tcgtggacgc cgccaacgtc
    15601 gcccaatacc tgaagaagcc ctgacgcgtg acgcccgttc tcgaactcca cggcctggcc
    15661 aagcgctacg gcgcggtcga tgccctgaaa ggggtggacc tcactgtgca tgagggcgag
    15721 gttctggcca tctgtggcga caatggggcc ggcaagagca cgctgatcaa ggtcgtcgcc
    15781 ggcgctcatc cgcgttcggc gggtgccatg cgcttgtcgg ggcgcgacgt gcagtggcgc
    15841 tcgccgcatg acgcgctggt caacggcgtg gccaccattt atcaggatct tgccctggcc
    15901 gcgcggttga gcatctggca gaacatcttc atcggctcgg agcttcgccg ccgcatcgca
    15961 ccgggctgtc tgttgctcga caagaaggcc atgcgcacgc aggccgccgg ctacctgagg
    16021 cagttgaacc agaccattga cgatgtggat cggccggtgt cggacttctc gggcggccag
    16081 cgccaggccg tcgccatcgc ccgcgcgctg cgctggaagg cgaaactggt catcatggac
    16141 gaacccactg cggcgttggg ggtgaaggaa accgccgagg tcctggcgct tattggccag
    16201 ttaaagacgc aaggcgtgac ggtgctgctc atcagtcaca acatggaaga cgtggtgcgc
    16261 gtcgctgatc gtgtggtcat actgaaggcg ggtcgagccg tggcgggggg cccgacggcg
    16321 ggcgtgtcgc ccaccagcct ggcccaggcc atcatcaccg ggtgctggcc ggggtgattc
    16381 tgccaaggat cctcggaaca agtcgagcgg caaacagccc tgaaccacag atgcatgggg
    16441 cacgatgact tgttcctggg gttcttaaga cgccccagga tggtgggtac tgtgtaggcc
    16501 ggcgcgtcca gcggtcgtgg cgctggccga accgccggcc tacacagtac ccgccatcct
    16561 ggggctgcga aatggcatgc gtggggggac tatgcggggg agtgtgtttc tcaagttgct
    16621 gcggcggctg gtcggcgaat caagaacact gccaccgccg cgactaggca ggcgccgccg
    16681 gcggcataca gcgcggggtt gtaggtcagc agcagagtgc gcaccaggcc cgcgccatag
    16741 gccgcgatgg cgccgcctat ctggtgtccg gtgaagatcc agccgaacac caatcccgcc
    16801 cgcaagcgcc cgaatgtggc gctcgtcaac ttcacggttg gtggcacggt ggcgatccag
    16861 tccatgccgt agaacatcgc gaacagggag agcccgtaga acgtgaattc ggagtgcggc
    16921 agccagaaca atgacaggcc gcgcaagccg tagtaccaga acagcagttt gcggttgtcc
    16981 agccggtcgg agagccagcc cgatgcgatc gtgcccacca cgtcgaacac acccatcatc
    17041 gccagcaccg aggcagcggg caactcggct agcccgtagt cgccacacaa cgagatgaaa
    17101 tgcgtctgga tgaggccact ggtgctcagt ccacagatga agaaagtgcc gaacagcacc
    17161 cagaacgtgc gattgcgtga cacggcgcgc agcgtggtga ggggttccat gaatgatggg
    17221 cgcgaggccg tgcgggccat cagcggcttc gcacccggct cggcgccata cggcacgctg
    17281 gacacatcct cggggtggct gcacaccatg cagtacacca atccggccac caccaggcaa
    17341 ctcgcgccga ccggaagcaa tgccacacgc cagcccacgt gttcaatgag ccaggccgcc
    17401 agcggcaaga aggccagttg tcccgtggac gaactggctg tcaggaggcc gatgaccaga
    17461 ccacgacgct tatcgaacca gcgattcgcc accaccgccc ccagaacgag cgcgatcagg
    17521 ccggccccca gccccaacac caacccccac agcactacca attgccagac ctgcgtcatc
    17581 attgtggccg acagcaacgc caccgtgatc aacaccaagg ccccgcacat cagcgtgcgc
    17641 agttcgtagc gttccatcaa gatggccgcg aagggcccca tgagcccgaa cagagcgaat
    17701 cgcaatgcca gcgccgatga tatctgttca acgctccagc cgaattcatg cgacagaggt
    17761 cgaatgaacg cgccaggcag gccgatcgcc gccgccgaaa ccagcatggt gagaaacacc
    17821 agcaacgcca tcatccaggc gtagtggata ttgcgccggc caagccacct cgcggcgtag
    17881 gttgcgaaca tggggacatt tcccgtcgat aagaagacgc tcattatcgc gacggatcaa
    17941 catgacgtaa atgacggaaa agcctcattt cacgacatca atgcccaggt gcccggacca
    18001 cgggcggcga aagggcctac aaccccagat aggcctgcct gacattatcg gaagacagga
    18061 tttcctcggc attgccctgc aatgcgatgg cgccgctttg catgacgtac gcgcgggcgg
    18121 cgatttcaag cgcctgcgcc atgttctgct cgaccagcag gatggatgtg ccggaacgat
    18181 tcaggtcggc cagcgtgtcg aacaccgtct ccaccagcag cggggaaagc cccaggctgg
    18241 gttcgtcgac cagaagcagc cgggggccgc tcatgagcgc ccggccgatg gccagcatct
    18301 ggcgctctcc ccccgagagc gtgcccgcga tctgggcgcg ccgctcggcc aggcggggga
    18361 acaaggcata gacctgatcg aggcgttcct gatagaccgc ccccttgcgg acggtgtagg
    18421 cgcccagacg cagattctgt tcgaccggct gccgggcgaa cacccgccca ccttccggaa
    18481 tgagagcgat gccgcgccga accagcgcgt caggcgtcaa agcggtgatg tcgtcaccgt
    18541 cgaacgtcac gcgccccgcc tttggcttga cggaccccac gatggacagc agcgtgctgg
    18601 atttgcccgc cccattggcg cccagcagcg ccgtgatttc gccatggcgc acctccagag
    18661 agatcccccg caccgcctgc acgccgccgt aggccacatg cagatcgtcc actttcagca
    18721 gcacactatc cggcacgata tttccttccg aggtaggcct cgatcacggt gggattgcgc
    18781 accacatctt cgggcgtgcc cgaggcgatc agttgcccga agtccagcac caacacgcga
    18841 tgcgccaggc gcatgacgac ttccagcacg tgctcgacaa tgagcagcgt gacgccgccc
    18901 gcgtgaatgg accgcaggat ttccgccagg gccaccgcct cgctggggtt gagcccgccc
    18961 gccacctcgt ccaacaacag catcttgggc gaactcgcca gcgcccgggc aagctcgatg
    19021 cgcttctgcc ccgccacgtt cagttccccg gcgggtgtca tgccgcgatc agtcagtccg
    19081 accgagtcga gcacccgcca ggcgacctcc cgcgcggcat cgagcgtggg gtgccgcagc
    19141 aacgcgccga ccatcacgtt ctcgagcacg gtcatggccg cgaagctgcg cggtatctgg
    19201 taggtgcgca tcaagcccat ggcggcgatg gcctcgggcg tgcgccccac caattccttg
    19261 ccatcgaagc gcacgtgccc ggaggtgggc gcatgatgtc cggccagccc attgaacagc
    19321 gtgctcttgc ccgccccgtt cggcccgatg atggcgacga tctcgcccgc ttcaacgctg
    19381 aggttgatat cgcgattggc gatcaagccc ccgaagcgtt tcgtcaggtt ttcaacttcc
    19441 agaagagcca cggcgaaacc tcaaggtaat caggcctttc ggcaggaaca ggctgaccag
    19501 gatgatcgcc aggccataca gcagcaggtc cagtccgctg cccgagctgc ccagcgccac
    19561 acgcatgccc tcgccaaggg ggatgagcac cgccgcgccc agccatggcc ccaccaggct
    19621 gccgatgccg cccagaatgg ccaccagcac gatctggatg gacagtgtga ggctgaacgc
    19681 ggaatcgggg tcgatgaagc cgatgtagat cgcgtagaac ccaccccaca ccccggtgat
    19741 cgcggccgag agcacgaagg cccacatctt gtagcgttcg gccggcacgc caaggctgcg
    19801 cgcggcggcc tcgtcgccat tgatggcccg ccagtagtac ccggtcttgg agcgcgccag
    19861 cgccacggtc gcccacagcg tgagcagcgc cagcccgaac gctatccagt agtacggcgc
    19921 cttggagcga aacagcagca gccacgcatt gttggcgatg ggcgcctcca ccccggtcgc
    19981 gcctcccgcc caggaccagt tgaccaccag cagttgcgcc accgacagca gcgcgatggt
    20041 cgccatggcg aaatagtgcc ccgtgagccg cagtgtgggc cgtccgacga tgaacgccac
    20101 cgccgccgcg accacgcccc cgagagggat gcccagccag ggactcaggt gccagttctg
    20161 aaggagcaat agggtcgtgt aggcgcccac gcccagaaac accgcgtggc cgaacgacac
    20221 ccggcccagg aagccaccca ccaggttcca ggcggcgccc agcgcgccgt acatcagcag
    20281 catcaccacg atctgctggg aaaacggatc gggaacgagg aacgggaaga tcgccagcgc
    20341 cacgatgaca atgacgagcc agatcagggc cgtgcgccgg cgcgccggcg cacgcggatc
    20401 gggcatgaca ccggcagccg gggacgcgat gcctgccgca ggcggcgggg cgctcccagc
    20461 gggtggcgcg gcaccttcct ggcgctgcgg ggaaagctgg ttgggttcgc ccatgtcacc
    20521 acctgccgaa aagaccgcgt ggccgatacc agagcaccag caggaagatc aggaagatgc
    20581 tgaccatctt gtacgcggga aggaacaaat agccggtcat ggattcgatg acgccaatga
    20641 gaatccccga tatgaaagcc cccggcagcg agccgaaccc gcccatggcc accgccacga
    20701 aagccaacag gccgaacacg ttgcccaccg aagggaacac atagaagaac gtcgtgagca
    20761 atgcccccgc aagccccacg gcggcgctgc ccaggatcca cacctgcgcg ttgacccggt
    20821 cggacgcgat gcccaccagg gaggccgcct gacggtcctc ggccaccgcc tgcagcgcat
    20881 gcccccagcg cgtgcggtac accaggaaga acagcagcac gatcaccacc agcgacacga
    20941 tcccggtgcc gacctggggc agtcccaggt tgatgccacc cacgctcaca ctgccagaca
    21001 gccaggtgtt gggcagattg cgataatcgg gcgagaaggc aatgaacgcc agctggcgca
    21061 gcaccagccc caggccgaaa gtggccagga tgacgatcat ggggggcccg cgctgcaacg
    21121 ggcggatgat gagcgcgaac gacagaaagc ccacgaaccc cagcagacac gccaccaggg
    21181 gcgccgacaa ggtggggtcc acatgtccca gggtgaacag ccaataggcc gcgtacatgc
    21241 ccagcatgag gaaatcaccg tgcgcgaaat tgatgacgtc ggtgatcccc cagatcaacg
    21301 ccaggccgac cgcgatcagc gcatagatca aaccgttcaa caaacccgtg actagcgcct
    21361 cgaacacggt gatccccttg ctgagttatg aatggactgg cgtccgcgga tatcggtgtg
    21421 gatgacgatc cgcaccgata tttcgcttat ttgccccagg tgaacggcag cttgggcaga
    21481 tccgccatct ttttgtagcg cgcgggccag acggtgtggt attccgtgcc ctcgagttgc
    21541 aggatcaggc cctgcccctc cacgttctgg ccggtcttgt cgaacttgac gccctcccag
    21601 ggcatgatga tttgattgac ggtcaggttg gtggcggcca gcgcctggcg gatggcatcg
    21661 ggcttggtgg agccggcgcg gttgatggca tcggccagca ccagcatgcc ctgcaccgag
    21721 cgcgcgctgt tgccgttcag gttattgccg gtcttggcct tgaacatgtc gttgatctgc
    21781 ttgatgatgg gcttggccgt gtacaggtcg ttgccccaga cgtcgcgggt cagcacacct
    21841 tgcacctgcg gccccacttc ggaaatgaag cgcgggtcga tgaagccggc atcattggcc
    21901 aggaagatgg gcggcgcgta tttcatctgg cgcatggtgc gcacaaacag catggcgtcg
    21961 gacgtgtagc tggcgaagat ggcgacgtcg ggtttggctg cggccagctt ctgggtttcc
    22021 gcgttgagcg acggcgcccc cgccgaatac gcgatattgg ccacgatggt gcggcccgac
    22081 tgcttggcga atttctcgac agccttgtag gtgttcaccc cgaagtcggt gttctcatag
    22141 acgacggcga tcttcttggt gggcaggttc ttgataccgt ccaggaactg catcatgttg
    22201 tggatgaagg tttcgtcgtt cggcgtagtg cggaagaacc acttgtagcc gcgctcggtc
    22261 agttcggggc tgctggattc cgggttcatg tagggaatct gacgttgctc ggcgatgcgg
    22321 ctggtggtct tggtgaccgc cgactgatac gccccgacca gcgcaaccac gtggtcgtgg
    22381 tcgatgaggc gctgggcctc ggcctgcccg acgctggggc tgccctgcga gtcggcgaaa
    22441 atgaccttga tcttggcccc atccagtttc ggcaggccgg cgccggccgc cagggggata
    22501 cctttgagtt cggggtgctt gttgttgacg atgtcgaccg ccagttcgat ggcctgcttg
    22561 agctgcgtgc ccgtggaggc gagcgcgccg gacagcgggt agatggcgcc gatggtgatg
    22621 accttatcct gggccgttgc attctgcgcc aacgcaatgc ccaggcagga aagcaatacc
    22681 gcaagcaact ttttcatctt gagtctcctt tgaaacgaca atcgtggttt ttatgatcgg
    22741 tgtcgtgcgc gacccggccc acccgcacgg gcgtggctca agtcaacggg cggggtagtg
    22801 tccggccgca tggtgtcatg gtaaagagtg accgcgcgcc gcgcaagaat ctttccagaa
    22861 ggcgagggga aaccagtgca tcagaacgcg gccagcgctg aattcaagag gtcggtcacc
    22921 agcatgtggc cgggcttgtg tgtgatggcc agcggcaacc gggccgattt gatggcttcc
    22981 tgcggggtga cgccgcaagc ccagaacacc ggaagttcgt cggcatggat ttccaccgcg
    23041 tctccatagt cgggcgcctg caagtcagcg atgcccagca gggacggatc gcccagatgg
    23101 accggtgcgc catgcacggc cggaaaccgg ctggtgatct ggatggcgcg gatcgcgttg
    23161 cggctcgtca tggggcgcat gctgaccacc aggttgccgc cgaatggccc cgctttcccg
    23221 ttgggcacag aggtacggta catcgcgatg ttgcgcccct cttggatatg gcgcagcggc
    23281 acgccggcct gcatcaacat gtgctcgaac gagaacgagc agccgatggc gaacaccacg
    23341 aaaccgggct gccagatctc gcgcagatcg gtgagctctt cggcgcgttc gccattgcga
    23401 tacacgtaat aacccggcag atcggtgcgg atgtcgatgt cgtcgcccag catgggcatt
    23461 tgccattgcc cgggctcgcc cacccccagc agcggacacg gcttggggtt gaggtgacag
    23521 aaacgcatga agtccgccgc ctgttcgcgc ggcaggatgg cgagattggc ctgggcataa
    23581 tcgccgcaaa cacccgccgt gggcttgcgg aaatggcctt gccgaatctg gtgtcggaga
    23641 tcactgggag tcatgccgaa acctcgatgg tgaaaagaaa aacaggattc atgcaagccc
    23701 ccgccggatt cgagggcgcc gtgcctgctc ttgtgttcct gattgaatga tagtctcccc
    23761 tgggatcata ggaacaatta aaattttgtt ctacctactt tttttacgcc atgttgtata
    23821 ttttttctta acaccattcg accatgaaca cccgattcat cgaaacgttc atcatgctgg
    23881 cccgtctcaa gagcttccgc gccacggcca acgcgctgca cgccacgcca gcggccatct
    23941 cgcagcgcat caaaagcctg gaagacgatc tgcgtacaga actgatagat cgcaccacaa
    24001 agacattccg tcttaccgcc aacggcaacc gtctgctcgg ccacgctcgc tcgatcatgg
    24061 acgcagtgcg caagatggaa gccgccgcgc accaggaagg caccgtgcgc ggctgcctgc
    24121 gcctgggcgt caacgaaacc gtggtgcata gttggctcgc ccagtacatg acactgctca
    24181 accgaacgta ccccgaactc gaggtcgaac tttcggtgga gatcagcagc gtgatgcaga
    24241 agcttctgct ggcgggcgac ctggatctgg tcctgcgcgt ggaaggcatc gacaacgaga
    24301 agatcgtttc cgacgcgctg gccatctacc cggtacgctg gatcgcccgc aaaggcttcg
    24361 tgccccaacg caagggcgac gccatcaaga ccatgctgcg ctaccccgtc ttgacgttcg
    24421 ggcgcggcac ggcgccacaa cgcgccatcg tgcagatcgt gagcacgctc gccaatcagg
    24481 ccgacatccc ggtcggccag gcccgtgtga tgtgctcacc gtccgtcgcg gccatcgtcc
    24541 agttgatacg cgacggcttt ggtgtcgccg ccatccccgc gcttttcgtc gccaatgaga
    24601 tcgacagtgg cgcgttcgtc gaactaccca tacagcccac gccaccgtcc atcgtcctgt
    24661 ccatgtgccg acacgctgat gccgacaggt acgtgcatgc tgcggccgac gtggcgcgcg
    24721 cggcctgcgg agaatattgc cgacagactg acagacggct ggtggaggcc gtggcggatt
    24781 agggtctatc gcgctacggg gcgcccttcg ataaggcggg ctccagaaca cgcatgagcg
    24841 ccgaccacat tgatgagaca gtacactgac acgacccacc gccattctcc tgacgccgcc
    24901 aacctgggcc tgccaccatg agccagcaaa caaacatcta tccgcatgca gtcgactcgt
    24961 cccgcctgtt caccatcaga tgtgcagccg cattggcttg cgcactggct ttggccggca
    25021 tgcggccggc cacggcggca tcacgctccc ccgcgcacgg cacacatggc atggtggtca
    25081 ccgcccagca cttggccacc caggtgggcc tgaacatcct gaaggccggc ggcaacgcca
    25141 tagacgccgc cgtggccaca ggctacgcct tggccgtcgt ggacccttgc tgcggcaaca
    25201 tcggcggcgg tggcttcatg acactgcatc tggccgacgg ccgcaacatc ttcatcaact
    25261 tccgcgaacg cgcgccgctc agggccacgc gcgacatgta cctggacaag cagggcaagg
    25321 tggtgccggg gctcagcacc cagggttacc tggcggtcgg cgtgccgggc acggtgatgg
    25381 gcctggacac cgccttgaga aaatatggaa cgatgacgcg caagcaggtg atggcgccgg
    25441 ccatccgcct ggcgcgtaac ggcttcgtgc tggagcaagg cgacatcgac atcctcaccg
    25501 ggaaaggcaa gcatggcgac ttggacgccc tgaccgggcc gagcaagcga ttcgccacgc
    25561 aacccaacgt cgcggccatc ttcctgaacc acggccaggc ctatcgggtg ggcgaccgcc
    25621 tggtgcagaa agacttggcc cgcactctgg cattgatcga aaagaaaggc tcatcggctt
    25681 tctacgatgg ccccatagcc gatgccatcg tccacgccag ccaggccaac ggcggcattc
    25741 tgggcaaggt cgacttcacc aattaccgcg ccgaagaagc ccggcccatc gaatgcggct
    25801 accacggcta ccaagtgatc tcggcgccgc cgcccagctc cggtggcacc acgttgtgcg
    25861 agatcctgaa catcatcgaa ccctacccgc tcgacaaatg gggtttccac tccgcggccg
    25921 ccacccacgt catggtcgag gccatgcgcc atgcgtacgt cgatcgcaac accttgctgg
    25981 gcgacccgca cttcgtgaag gcccccatcg cgcgcctgct gtccaaggca tacgcggccc
    26041 gcatccgcac cgccatcctg cctgacaagg ccacgccgtc gagcgaggtg aagcaaggcg
    26101 tggcaccaca cgaaggcacc aacaccacgc actactccat cgtcgacgac aaaggcaacg
    26161 cggtatccgt cacctacacc atcaaccggt atttcggggc gggcgtcatc gccggcaaga
    26221 caggcttctt cctcaacgac gagatggatg acttcacgtc caaggtgggc gtgcccaatc
    26281 tgtacggtct gatccagggc gagaacaacg ccatcgcccc cggcaagcgc ccactcagct
    26341 ccatgacgcc gaccatcgtc accaagaacg gaaggctctt catggtggtc ggcagccccg
    26401 gcggctcgcg catcatcacc atcaccctgg aaacgtttct caacgtcgtc gatcacggca
    26461 tgaccctgca ggaggccgtc gacgcgccgc gcttccatca ccaatggcag cccgatgtga
    26521 tccagtacga accggacgcg ttcacgcccg aagtcatggc cagcctgaaa gccatgggct
    26581 acaaattcaa ggcctatggg ccttggggcg cggcggaagc cattctggtg aatccggcga
    26641 ccggcgcgct gaccggcgcc gccgacaaac gccaggcagc gggcgcggcg atgggatatt
    26701 agctcgcctc cagggctcag tactgcgggg cctaggtcca gcccaccctg gagaacttcg
    26761 acacgcgggc gttgtgcaca ctgcatcagt ggccatcgcc cgcgatcccg agccagacga
    26821 tcaggcgttg aatcgcgcga gcgcttcggg tgtatcgacg tcggcaacgc acccggcatc
    26881 gtcgacatcg atctgaacag cccgcgtgcg ataagtcttg aacagtggac cggcgccttc
    26941 ttcaggccgc agcgccatca gatcgggccg caacgacaca gacagggcaa ccgggtgtcc
    27001 tggctggccc cgataaacag ggcgcgcgag caatgcccct agcatcagag cctggcgcaa
    27061 ggattcgtag gtggccctgg aaatgtacgg catgtcggcc agtgccacca accagcccag
    27121 gcgcgggctg gaacaggaga tcgcgctttg cagactgcga cccatgccct gtccggcctg
    27181 gaggcaactg aactggcgcg cgggcagttc cgcaacgacg ggatagcttt gcagtgagcg
    27241 cgggccatgc acgatcaaca gctcatcagt ggccgcaagc agcttgccgg cgctgacctg
    27301 caacaggttt cgccccggca ataccggggc cagcaacttg aaggcccggc cgctggggtc
    27361 gtagcgcctg ccgatgccgg ccgccaggag aatgccggca acgtccgccg atgagtgccc
    27421 ggaaccttcg ggcaccgagc acgccatatg ccctggcatt gcgggcgctt tatcgtccat
    27481 tggctccatc gcgaaacgat acgccgaatt cacgcccagc gtcagagtcc gacatcattt
    27541 cctcccgagt cggtctcggg agtgatcctg tttcggataa gcaaacgggc gaagacgcct
    27601 gcggttcttc aaccatcgga gcgggaagaa acataaccac cgggagccgc catgtatctg
    27661 ggaagttgcc tatgccaggc aatccgccac gagatcagcc aacacgcggt gtttcctgat
    27721 ccaatgtttg tgataaccca aaaactggcg cgcccggagt gagatacatc gaaccttcag
    27781 gacgaaattt tggaggaact ggcaagctgg gacgccgaac tcaagcgcat taactctccg
    27841 gtgctgcccc cgaacaaagg gggctagcca tgagcctaga ccgcccaccc atcactgaag
    27901 agtttcggga tgccgtcgaa cacaacgcag cccggacccg ccacgccata tccccaccgc
    27961 cattttcctt gcgtctgact gccgcagaaa agacggaatt gctgcgccgc gctgggagta
    28021 tgccgcttgc ctccttggct cggaggccgc gcctgtcgcg gcctttgtga agaccggaag
    28081 aagcgcaaac ggcgagtagc ggctggcacc cgcaagggcc gcaacaacgc ggaggaaccg
    28141 gaaaggattt tcggtttgca agtgccatag ccttgtgcca gaaacggcga gaaaaggccg
    28201 tgcaggcggc ggcagggagg cagaaggagc ggaaacgcgg tttatcttga cgtgtacgga
    28261 caaatgacgt acattggact atagaacatg aacccattcc ctgtgaggtt gccaatgctg
    28321 catgcacctg aaatccaaga aaaatcgcgc aatgcgcgtc tggaggcgcg cgtgtccagc
    28381 gaccagaagg actttttcca acgcgcagcc aatctgaccg ggcgcagcct gagtgagctt
    28441 gtcatcgaca gcacgcaaga agccgccgcg aagatcgtgc aggagcacga agctatccgc
    28501 ctgagccgtg aggaacaggt ggcgttcgtg agtgcgctgc tcacgccctc tgaaccgggg
    28561 ccgcgcctgc aaaaggccgt ccagagctac cgtcagaagg cggggttttg attggcgctc
    28621 attcttgcac cggagcacat caatccgctt accaagtccc atcagcgcaa gtcattcgat
    28681 tgcggccatg aaaacctgaa tcgttatctg cgcgaacagg cgcggcagga cgctgaaaag
    28741 cgggttgccg cgcctttcgt gctaacgcag ccgggcgatc ccaaagtgct cggcttctac
    28801 accctttcat cttccatcat tcccgtggac gaactgccgc cagacctgat gaagcggctg
    28861 ccgcgctacg gccagttgcc cgtcacgctg ctcgggcgtc tggcggtaga tcactccgcc
    28921 ggaggtcaag gtgtcgggga gttcctgctg atcgatgccc tgcgccgcag cttagaagct
    28981 gcgcagcaga tcgcagccat ggcggtgatc gtagacgcga agaacgagcg ggccgaaagc
    29041 ttttaccggc attttgattt ccagcccttc cagcaaaccc cgcttcggct gttcctgccc
    29101 atggcgcaga ttgcaaagct gtttgcatag caacttttca gtaaataatt gattacctct
    29161 cagaaacgtg ggaaacaatg agggggggga gaagaatatc gtgtttgaac aaacttttaa
    29221 gaatatcgat gacatcctgc ataaagatgc aggatgcacg agcgaactgg attgcggtac
    29281 accgctgacg gcttgctggt ccaagggacg catgagctat cacccctact atctatgtca
    29341 ctctcgcggc tgcaatagtt acggaaagtc catccgccgc gacgttatcg aagggcagtt
    29401 cgaggacttg ctgaaagaga tgcagccctc ggctaatctc ttgcaagcgg cacgggctat
    29461 gttcaaggac ttgtgggatc atcgccttgc aacaggcgca acccgcgcca aggcgctcaa
    29521 gaccgaactg gcgaaggtca cagaacaggt agaacagttc cttgaccgaa tcgccgacac
    29581 gaccatcccg tccgtagttg tggcctacga aaaacgcatc aagagtctgg aagacaggaa
    29641 gatcgtactg atcgaaaaca tcgcccattg cgggcgaccc gtgcgaagct ttgaggaaac
    29701 acttagaacc gccttcgatt tcctcgcaaa cccttgtaat ctatgggttt ccggacgtct
    29761 ggatgacaag cgcacagtgc taaaactggc cttcgccgac cgcttggcgt acacgaaaag
    29821 agaggggttt agaacccccc atttttcctt gcctttcaag gctttaacta actttgaacg
    29881 gcaagatttt agaatggcgc gcccgacagg aatcgaacct gtatctagag cttcggagac
    29941 tcttattcta tccattgaac tacgggcgcg aagctcgcta ttgtagcgtg tttacgggaa
    30001 cgattttgtc agccacgacg gcgccagccc gacaaaccgc gcaccaatga cggaaatcac
    30061 tgcatccccc tggcagtctc gctataatcg ttgactatgt gcttgatcag gcagggtttg
    30121 gtgcgcgttt cacatgcact cttcaaccga tgccgggtgg tgcgattgat gatagaagcc
    30181 ctattgcact cctgttgctt gcgcttttga gctctgatgc acattgccga agcgcgccgc
    30241 gcacgactgg cgacgcgtcc ttgatattga acgcaggatt cgaatatgac cagcacggaa
    30301 gagcacaaag aatcgcacga gtctttcatc aagacacctc ggcaactgat cgtcacgatc
    30361 atactggcct tccttatccc catcatcatc atcatcatgc tggtgcacat ggtggtggtg
    30421 tccacgcccg ctggcgccgg ctcggatgcc gattcgacaa aaaccattct ggcacgcatc
    30481 cagcctgtgg ccagcctgaa gctggtcgat gcgaacgcgc ccaaaaccta tctcaccggc
    30541 aagcaggtct tcgattccac ctgcacggcc tgtcacaccg ccggtgtcgc cggcgccccc
    30601 aaagtgggca acaaggccga ctgggcgccg ttcatcaaga ccggctacca ggaaatgatc
    30661 aagaacgcca tccacggtgt cggcgccatg cctcccaagg gcggcaacgc ctcactgagc
    30721 gactacgaag tcgcccgtgc cattgcctac atggctaacc aatccggtgc gtcgttcaag
    30781 gaaccgcctc cgccagcacc cgcctccgcg aagaaagtcg cggcggcggc agtgcctgct
    30841 ccggtagtgg ccgcaaccgc gcccgccgcg gccgcgccag cccaacccgc ggcggaaccc
    30901 gccaaggccg cggcccaacc tgccgcggag cccgccaaag ccgcaccagc ccccatcaag
    30961 gtcgccgcca tcccgggcct ggcccccgac gcggccacca accccgaggg tgaaaaactc
    31021 tacaagactg tgtgcttcgc ctgccatgcc accggcgtca tgggtgcgcc cacgttcggc
    31081 agcatggaaa aatgggggcc gttcatcaag accggcatcg acaccatggt caagaacgcc
    31141 atccacggtg tgggcaacat gccgccacgg ggcggctcgc aggccaccga tgcgcagatg
    31201 cgcgccgggg tcgaatacat ggtcagccac gccaagtaat cctctacccc cgaaaagggt
    31261 gccgggcggc gcgaccgtcg tcacgaaaag ccaggctcac ggcctggctt tttcaattca
    31321 cgttctgtac cgcacaggac tcacctggtc gtggcgcgcc acggggctca ctcgctcatc
    31381 gcgtcgcaca gtggttcggc acccagcgac agccccagtt gcacacggcg ttgcagccac
    31441 aggctggggc ggtaacgcgc atcgcccgtg acgtgccgca tggctcgtag cacttccagc
    31501 acatgtgcgc tacccaaccc gtcgcccagc gacaacggcc cgccccctgg ataacccagc
    31561 cccagcgata cggccaagtc gatgtctcgc gggctggcga ttttctgttg tgcgatgtcg
    31621 ctcgcgacat tgatgatggt ggcgaccatg cgctggccca cgaagcccgg cgagtcgttg
    31681 atcaccgaca cggcggtgcc atcgctggcc atcaacgcgt gggcggcgtc gcgccagcgc
    31741 ggctcggtgg cgggcgagca catcaacacg cgccgcctgc cctgctccag gccgaagaac
    31801 gtgtcgagcg ccacggtgcg tgtcgcgtcg agctgatgcg cctgcgccac cgtggcggtg
    31861 tcctcgccaa agggggtcac cagaatcagc gcatcgggtg gcggcgcact acccgtcagc
    31921 acctgcatgc ccagagactt gagcaacgtc gcggcgcgct tgtggcccac ggagtgaaat
    31981 ggcgccagcc agactttcag cccgtcgggt agggccggtg gcgcgggctc gggcggcacc
    32041 aacttggtgc cgttttcata gcgatagaag ccctctccgg acttcctgcc cagcatcccg
    32101 ccggcgaacc gcacggcagt gacaggcgac gggcgaaaac gcggctcgtc atagaattgc
    32161 ctgtagatgg attccatcac gggatgcgaa acgtccagcg cggtgagatc caacagttcg
    32221 aacgggccca tgcgaaaccc cgcctgctcg cgcatgatgg cgtccacatg atgaaacggc
    32281 gccaccgcct cttgcgccag acgcacccct tcggtattca tgccgcgacc ggcgtggttg
    32341 acgatgaacc ccggcatgtc gcgggcgcgc acgggggtgt ggccgagttc gcgggcaagc
    32401 gccatcaatg tatcgcccac ggcggggtcg gtccgcaggc cgtcgatgac ctccaccacc
    32461 ctcatgaggg gaaccgggtt gaaaaaatgg tagccggcca ctctttgcgg taaccggcac
    32521 gcggcggcga tggcggtgat cgacagcgag gaagtattgg acgccaggac gcagtcctcg
    32581 gccacgatgc cttccaaccg agagaacaat tcctgcttga cgtcaagccg ttccacaacg
    32641 gcttcaacga ccagatcagc cttggccaac gcggccaaat cggcgcaggc cgtgacgcga
    32701 gccagcgact gcgcggcggt ttcgcccctg atgcgcccct tggccacgag ctttccccac
    32761 atcgcctcga ggtggccacg cgcggcttcg accgcctgcg cgttcatgtc gaacagcagc
    32821 acgttacgtc cggcctgggc cgcgatctgc gcgatgcccc gccccatggc gcccgcgccg
    32881 acgatgccaa tggtgtgaat agtcttcatg ttcgatctcc gtagcgccca ggcctaccgc
    32941 tgggcgggac gagaggacat gtcgtgcggc gcccgtatga caaaaaggct attgtgcccc
    33001 aaaccatcta cccgcataaa aaacccagcg cgaagcaagc ctgttgcggc gttgcactga
    33061 gcatgaatca atactaggga aaacccttga tttacgatta gggttttccc tataatgaca
    33121 cctatcaact caccaacaca gcaggtatcc atcatgtcgg tcaatgctta cgatcaatac
    33181 gtgaaactca gcgactctct cgaacgcgaa ctgacacgcg aagcgctgag ctgcggtgaa
    33241 ccggtttcga tcgtggatgt ggcgaaaagc atcgcgaagg gcgtcaagat caccgttcgc
    33301 gctatcgccc gcgtactgtc ggacgccacc cgtcccctgg gcaacgccca ctcgcacgac
    33361 aaccagttga gcagcgcgcg ctggtaagca tcaatctttc tttcggggcg cgtcgagcag
    33421 atccttgagc atgccaagac gctgactggg gctgagcgca cgcaacgcgg gatcctcggt
    33481 catgccggcc tcctccagcc ccatttccga aataaagcgc gacacttccc gcaccacgtc
    33541 gtcgcgcgcc cggcgccgtt tgcggcacca gcttagatgc aggctgcgcc gggcacgggt
    33601 gatacccacg tacatcaggc ggcgttcttc ctggatgcgc tgggcaagcg cctcggccgc
    33661 ctgcgcggga tcggcatcct cttcgtcgcg cccggtgtgc ggcagtaacc cttcttccac
    33721 acccaccagg tagacatggg gatattccag ccccttggaa gcatgaagcg tggacaattt
    33781 cacggcgtcc ggctcttcat catccccgcg ctgcagcaat gtgaccagcg ccacgtgctg
    33841 ggtcagctcg atcaggtcga ggccgtcctc ctgggctttg cgcttcagcc agccaaccag
    33901 ctccagcaca ttctgccagc gtccctgcgc cgcgcgctcg tccaaggtgt cgaacaaatg
    33961 gcgttcgtac tggatcaagc ccagcaattc atcaagcagg gccggagcgg gctcggcctc
    34021 ggatcgatga cccggcacgg tttttccaac caccgactgt gcctcacccg gcggcgatga
    34081 cgcgccatca gtggtgaaca ggtccggctc ccgcgcgaat acctggacat tggatcgccc
    34141 cgcgcgccgc tggaaattct ggatcagttc gccgaacacg cgcagcgatg ccagttgcct
    34201 ggcgggcaca cgcgcctcgt cgatttcgta cagggcggcg aatagcgaca ggtgtttttc
    34261 cgctgccaac tgccccagcg cctgcagtgt ggcttgccca acaccccggc gcggcgtggt
    34321 caccgcccga atgaacgcgg gatcgtcgtc ctcgttcgcg atgaggcgca gatacgcgag
    34381 gatgtcccgc acttcgggtt tttcgaagaa gctctgcccg ccggatatcg tgtagggaat
    34441 gcgcaggttg cgcagcgcct gctccaatac gcgcgactgc tggttgctgc gatacagcac
    34501 cgcgaagtca cgccattgtg cccgacgttc gaagcgcgcc gccgaaatgc ggatggccac
    34561 tgactcggcc tcggccaggt cgtcgtccat cggggtgacc tggatgggct cgccctgccc
    34621 cagatccgac cagagtttct tgccgaacag gttcgggttg tgaccgatga cctggttcgc
    34681 cgcggtcagg atgcgctgca ccgaccgata gttctgctcg agcttgatga ccttgagcgc
    34741 cgggtagtcg gtggtgagct tggcgaggtt ttccaccgtc gccccgcgcc aagcgtagat
    34801 ggcctggtcg tcgtcgccca cggcggtgaa cagggaacgc atcccggtca gcgcgcgcac
    34861 caggtgatac tggcacagat tggtgtcctg gtattcatcc accagcaggt acttcacgcg
    34921 gcgctgccag cgttcgcgaa cctcgggatt ggattcaagc agcatggctg gcaggcgtat
    34981 cagatcgtcg aaatcaacgg cctgatacgc cgccaacgtc gcactgtagc tgcggtagac
    35041 tctggccgcc tcggcctcgc tgggcgtggc cgccgcgcgc tcggcgccat caggatcgag
    35101 ttgggcattc ttccacaacg agatcgtctg ctgcacggcg cgcaaccgag ccttgtcggt
    35161 cgtcgccagc aactcctgga tgatggccag ggcgtcatgc gcgtccagaa tagaaaactg
    35221 tggcttgagc cccgcatgcc tggcctcttc gcgcaggaaa cgcaacccca gcgaatgaaa
    35281 cgtcccgatg gtcaaccctt tgcccaggcg cgcatcgacc agcttgcgca cacgttcgcc
    35341 catttcccgc gccgccttgt tggtgaaagt aagcgcgaca atgtcacgcc cctgataacc
    35401 gcattcacgc agcaggtacg ccatcttctg ggtgatgacg cgtgtcttgc cgctgcccgc
    35461 tcccgcgagg accaggcacg ggccgtccag gtacatgacc gcttcgcgct gccggctgtt
    35521 gagatcgaca ggcactgtgt gcccgttgac gggatcgaag gccatgggac tcagatctcc
    35581 agcggatcga cttcgagttg gcagcgtgcg cgcgccgcgg cagccagggc gggcaaggtg
    35641 cgcgaccaag aggccaggaa cccctgcaac gccggacgat gacggctttc caccagcaac
    35701 tgggcgcgtt cgacattggc gacccgcacg acgcgcaacg gcacaggatc gtagagagtg
    35761 accgccgccg tatcgacatc ggaggatggc aacgtgcctg ccaacgcctt ggcccgtgcg
    35821 aggaagtcca cggcgatggc cagctcgggc gcctcggcgg tcagcaacgc ctggtacgca
    35881 tagggtggca gccccaccgc ctcacgctcg gcaaggctgt actgcgcgaa accgacgtaa
    35941 tcatgacgcg tcaacgcttg atagacgggc tgctcgggat agccggtctg gatgatgacc
    36001 tcggcgcccc gcgcgtgccg ccccgcccgg ccggcgacct gcatgagctg ggcgaacagg
    36061 cgttccggcg cccggaagtc ctgggcgaac aacatggcat cggcgttgag cacgccaacc
    36121 aaccccagat tctcgaaatc gtgacccttg gaaaccatct gggtgcccac catgatgtcg
    36181 atctccccgg catgaatgcg ggcgaacagc ttttcagcgc tgcctttcag acgcgtgctg
    36241 tcggcatcga tgcgcgccac ccgggccgac gggaacaggt cggcgagccg ctcttccacg
    36301 cgctgcgtgc cgcgccccat ggcctggaga tcctggtctc cacagtccgg gcaggcccgg
    36361 ggcgccggca tttggtggcc gcagtggtgg cactgcaaat aatggcggcg cgcatcgcgt
    36421 cgatgcaaca cggcataggc ggtgcatcgc ggacactggc tgacccagcc gcaggaatgg
    36481 cagttgagca ctggcgcata gccgcgccgg ttcaggaaga ccaacgactg ctggcccgcc
    36541 tccaggcgct gcgccagcgc gtcgatcagt tgaggcgaga acccttgctc cagcaccagg
    36601 cgccgggtat cgaccagccg cacgcgcggc gggtcgaccg gatgggcccg atgcggcagg
    36661 gttgccagca aataatggcc ccgccgcgca tgatgccagg attcgagagc tggcgtggcc
    36721 gaacccagca cgaccgccag ccccgcatca tgcccgcgcc agaccgccag gtcgcgggcg
    36781 gaatagcgca gcccttcttg ttgcttgtag gatgcatcat gctcttcgtc gacgatgatc
    36841 agccccagtt cgggcatggg cgtgaaaatg gacagccggg taccgagcag gatgcgggcc
    36901 tggccgcgcg acgccgacag ccacgcacgc aagcgctcgc cttcggacag cccactgtgc
    36961 agcacagcca atccgtcggc gcccgccaat ggcgcaagcc gggcgcgcag cgagcgctcg
    37021 agttgtggcg tcaggttgat ctccggcacc aggaacagca cttgccggcc cctggccagc
    37081 atgtcttgcg cggcatgcag atacacctcg gtcttgccgc tgcccgtgac gccgtgcagc
    37141 aagacggttt tgtgtccgcg cacggattcg atcatgtcgg ccgccaacgc ctgctcggca
    37201 ttgagtgccg gcgcctgatc ggcctcgatt tccttggcgc ccggtgcccg gcgccgatcg
    37261 atgcggcaca ccggaccacc agcggagcgt ttgccctggt aggccgatac tttgcgcaaa
    37321 ccggccggca acgcgggcag catgacctcg cccaacgggc gctgatagta ctgagccgcg
    37381 aatgtggcca accgcatcca gtcgggcggc agggggggaa ggtcatccag tatctgatcc
    37441 acgtcccgga tcaggccagc gtcgaccgcc ggcttttcag gtagcgacac cacgacgccc
    37501 ataagcttgc ggcgtccgaa cggcacgatg acacgctgcc ccaccgacgc ggtgtccggt
    37561 gcacgataat cgaataaccc atccagcggc acatccagcg cgacgcgcac ccagacggta
    37621 tcggccggca atgcggccaa aacgttgtca gagggtgagg tgaggtgggt catcaggtta
    37681 ggaaaccgag cgacaaaact taatgtgatt tcttactatc cgcgttaact gcttggcaac
    37741 acatcgactt tgaacgcgcg gccgacatcc tgtggataac tttgggggaa actggcggaa
    37801 caaccgggga aaagtcaacc attcctcgga tgtggaatag gtgttttccc cagaaaaata
    37861 aaaatattcg tttaaaatca acgtattaaa caaaattaaa aataaatccc ggcaagcgat
    37921 cgagaatggt gcgtagcggc aagacttgtg cacaacccat cgcgacaagc ccgaaaaacg
    37981 ggaaaatcgc catttatttc agtgcaccat cggcatggaa cgcccggcta tgcgtgtgga
    38041 cttcgtccac cagagtaccg acggcctcgg gaggcgtgaa gcgggaaatg ccatgcccca
    38101 gattgaacac gtgcccgccc tggccaaccg ggccgaagtc gtcgaggacg cgccgagcct
    38161 gggtccggat cgacgattcg tcaccgaaca ggaccatggg gtcgagattg ccctgcaagg
    38221 ccactttgtc gcccgtgcgc gcccgggccc gcgccaggtt gaccgtccag tcaagaccca
    38281 ccgcatcgca gccacaaccg gcgatgtctt cgagccaagg gccacccccc ttcgtgaaga
    38341 cgatggatgg tactgttcgg ccttcatggg tcttgatgag cccttgaacg acccgacgcg
    38401 tataggccaa tgagaactcc tggtacaggc cgtcagccag caccccgccc cagctatcga
    38461 aaatcatgac agcctgggcg cctgcctgga tctgggcgtt caaatactca caggcggctc
    38521 gagcgttgac gtcaagcatg cggtgcaaca ggtcgggccg ccggtacaac aggcttttta
    38581 tgatgcggta atcgccgctg ctcccgccct cgaccatata acaacccacc gtccaaggac
    38641 tgccggcgaa accgatgaga ggaaccttac cggcgagttc acgacggatg agcgcaacgg
    38701 catcgaagac gtagcgcaga tcgcccatgt cgggcacggt gagccgcgcg acgtcggcct
    38761 cggtgcgcac cggcgacgcg aaacgcggcc cttccccttc ggtgaaaccg agccccagcc
    38821 ccatggcgtg cggtaccgtc aggatatcgg agaaaagaat ggcggcatcc agcccgaaac
    38881 gattgatggg ctgcagcgtg acctcacagg cgtactcggg gttctgcgcc agccccagga
    38941 acgacccggc ccgcgctcga gtctggttat attcaggaag gtaacgaccc gcctgacgca
    39001 tgagccagat gggggtatgg ggcacgggct cgcgcagcag ggcacgcagg aagatatcgt
    39061 tggtgagtac gggcggggac acggaaatcc ttaaaaagcg gaaccgcccg acgacgggcg
    39121 gcgctggtga aacgggcgat gccctgcagg gattttaccc gtcttgccag gccgtcgccc
    39181 ccgctcatcc gtcacctatg catctgtctg cgattccgcg cggtatggcg ccgcttcgat
    39241 ctgatgtttg cgcatgagcg cccagaacgc gcgccggtgc ttctgggcgg atcgggccgc
    39301 catggcgatg ttgccggaat ggcggcccag ggccgcagtg atgtaggcgc gttcaaagcg
    39361 ttcaatgacc ttgcttttcg ccgacttgaa cgattcgcgc gaggtggccg agcgagaagc
    39421 cgtggcggcg gcgaatgcgt cgagctcgag gcctgagcga tcgaggatgc tggcgcacaa
    39481 cgcgtcttct gttacgcccg tgtgcggcga ggcgccataa tccggcgcgc attcgcccag
    39541 gccgccggcc ggcgcacccg gcaatagccg atacttcggg ctatcaagcc ggcgctcgac
    39601 ccgtgtgcgg agctcttccg tgcagacggg cccacgcacg aaatcggcga gccccaggga
    39661 atacaagtcg tcgagcgcgg ccgccttgag ttggtgggcc aacgcgatga tgggtgtgtg
    39721 cacccgcccc cgcgccgcca gcagactggt gcggacccag gcgagattgc attccgacac
    39781 ggtgatcaga caggcatcga agcggcgcag cgccatctcg gagcttgcca gcaactgact
    39841 ggaacccggc tgggccacat cgacctcgag cttcaacgcc tgcagacgca gccggcccgg
    39901 cgtgtgccga ccgtcaaacc aatgactgat ccctgagtgc gtacaggatg tgctcaagac
    39961 ggcacaatcg agcctttccc tcatgattcc ccctttttgt gttttatgat tggcgcacgc
    40021 gccccgatgc gtgttcatca cgacgacacg ggaacgagcg cactccggcc tgaatggcac
    40081 ccatcccgtg tagccatggc ctggaaacaa gaatacccag gtggccggcg cgggccaatc
    40141 cgcaaaactc tcaacagggg aactacgcaa gcgcgacaac acgcgtcaaa agacgggacc
    40201 ggtgcgaaca ggtataccgg tgcggtggag ggatggggag agggtccggt cggcgccgcg
    40261 cgggcggcgg caccgactgt gccggatctt tttagtgacg acgcaactga ttgagcttgc
    40321 gcgccgccgc gacctgcgcg gccagcatgg caagttcggc ctcgacggcc gcgatgtcct
    40381 ctttttcggt ggcgttgcgc aaagtttcct cggcgcgctt gcgtgcctct tcggccttgg
    40441 cctcatcgag atcggcggcg cggatcgcgg tatcggacag caccgtcacc acgccgggtt
    40501 ggatctcgag gataccgccg gcgacgaaga tgctttcctc accaccttcg gccaggacga
    40561 tcttgaccgt gcccggacga atacgcgaaa tcagcggcgt gtggccgggc atgacgccca
    40621 gctcgccggc ttcaccaggc agagacacga atttcgcctc gccggagaag atcgactctt
    40681 ccgcgctgac cacgtctaca tgcagattcg ccatgtgtca ggctccttga tcaaagcttc
    40741 ttggctttct cgatagcctc gtcgatggtc ccgaccatat agaaagcctg ctcgggcagc
    40801 gaatcgcatt cgccgttgac gatcatcttg aagccgcgca gggtttcagc cagcggcacg
    40861 tatttaccgg gcga
//
LOCUS       DASTNK010000126        60208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1021840, whole genome shotgun sequence.
ACCESSION   DASTNK010000126 DASTNK010000000
VERSION     DASTNK010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 60208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60208
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021840"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..1341)
                     /locus_tag="VFR20_09310"
     CDS             complement(280..1341)
                     /locus_tag="VFR20_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU0230758.1"
                     /translation="MRIPVLSHSSIFAALGAAVLFGVSTPLAKVLLHDLSPVLLAGLL
                     YLGSGVGLGVVRIARDRGWRPPGIARIEWLWLLLAIAFGGILGPLAMMLGLAHTSAAT
                     ASLLLNLESVLTALLAWVVFRENVDRRIVIGMLMIVSGAVLLTLPGATQAAHLGWGAL
                     LIAGACLCWALDNNLTRKVSASDAVFIAGLKGLVAGVVNIAIAVFMGTRWPAPQFVGM
                     AMVVGLLGYGVSLVLFVLALRGLGSARTGAYFSTAPFIGAAIGIFALGDPASWAFWLA
                     AVVMGAGVWLHLTERHEHLHTHEPITHTHRHLHDAHHQHVHDFDWNGREPHTHEHTHA
                     PLTHSHPHYPDIHHQHRHS"
     gene            complement(1353..1901)
                     /locus_tag="VFR20_09315"
     CDS             complement(1353..1901)
                     /locus_tag="VFR20_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEU0230759.1"
                     /translation="MATADSFTFKATLSRPVEPGNNGTWAFLVLPKSASETLPRRGRT
                     SVQGTINGHPFQATLEPDGQLSHWLRVGNELREAAGADFGDTVTVRISPADPEPEPML
                     PDDLLQALDAHPEAKAVWEATTTIARVDWVHWVESAKQAKTRKSRVENACDMLAKGKK
                     RVCCFDQSGHYSKSLSAPEAAK"
     gene            complement(2048..2138)
                     /locus_tag="VFR20_09320"
     tRNA            complement(2048..2138)
                     /locus_tag="VFR20_09320"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2102..2104),aa:Ser,seq:gga)
     gene            complement(2208..3455)
                     /gene="ribD"
                     /locus_tag="VFR20_09325"
     CDS             complement(2208..3455)
                     /gene="ribD"
                     /locus_tag="VFR20_09325"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU0230760.1"
                     /translation="MSRHDEHWMRRAIDLARQVMYLTAPNPRVGCVIVRDGQVLGEGA
                     TQEAGGPHAEVMALRDLHRHHLSAEGATVYVTLEPCSHHGRTPPCVEALIAERPARMV
                     VAMLDPNPLVGGRGLGRLRDVGIAVTTSICTEEALELNPGFVARMTRRTPWVWLKLAA
                     SLDGRSALNNGVSQWITGPTARADGHHWRARSSVVLTGLGTILADDPRLDVRLVETPR
                     QPIRAVVDPRFEIPEDARLFGGGPVWVFTARNDPEKAARLAAMGARAIFLPMVDDGAP
                     SIVDDVTWSKVDDVALSKADNSALRTADDGAGSRPRVDLNAMLRWMAEQEVNEVHVEA
                     GPGLCGALLGAGCVDELLVYLAPMLLGDAQPMARLPALASLDQARRFQFLDSVFLAPD
                     MRLRARCADRWQALLEAVQAPSA"
     gene            complement(3469..3930)
                     /gene="nrdR"
                     /locus_tag="VFR20_09330"
     CDS             complement(3469..3930)
                     /gene="nrdR"
                     /locus_tag="VFR20_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEU0230761.1"
                     /translation="MKCPFCHSFDTQVVDSRVSEEGDTIRRRRRCAACERRFTTYERV
                     ELTMPAVVKRNGTRVEFDADKLRGSLRLALRKRPVDTAAVDAAVARIEESLLVSGSRE
                     VSSGHIGELVMAELKRLDQVAYVRFASVYKSFEDIDEFVKAIDEIQLPPPS"
     gene            complement(3974..5218)
                     /gene="glyA"
                     /locus_tag="VFR20_09335"
     CDS             complement(3974..5218)
                     /gene="glyA"
                     /locus_tag="VFR20_09335"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU0230762.1"
                     /translation="MFDRSRTLDQVDADVWAAVQQENTRQEQHIELIASENYASPAVM
                     QAQGTQLTNKYAEGYPGKRYYGGCEYVDVVEQLAIDRLKEIFGAEAANVQPNSGSQAN
                     QGVYMAVLKPGDTVLGMSLAEGGHLTHGSPVNASGKLYHFISYGLDAKEELDYDKLEQ
                     LAREHKPKLIVGGASAYSLAFDFERMARIAHDNGALFMVDIAHYAGLVAGGVYPNPVP
                     HADFVTSTTHKSLRGPRGGVIMMKAEFEKAVNSAIFPGIQGGPLMHVIAAKAVAFKEV
                     LTPEFKQYAAQVKKNADVLAKTLVTRGLRIVSGRTESHLMLVDLRPKGITGKVAEAAL
                     GQAHITVNKNAIPNDPEKPFVTSGIRLGTPAMTTRGFKEAEAELTGNLIADVLDHPTD
                     ESVIAGVRARVHELTARLPVYR"
     gene            complement(5498..6130)
                     /locus_tag="VFR20_09340"
     CDS             complement(5498..6130)
                     /locus_tag="VFR20_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEU0230763.1"
                     /translation="MKLTSESFVDQQWIPPRHAFGKADAQAHVALADNMNPHLAWQDA
                     PQGTKSFVVICHDVDVPSEPDDVNQEGREIPSDLPRVDFHHWVLVDVAADTSHIAEGA
                     YSSGITPRGKAGPLALNGTRQGVNDFTSWFASDRDMSGDYFGYDGPCPPWNDSIVHHY
                     IFTVYALDISELPVRGRFTGVDARAAMKGHILGQAAITGLYSLNPRLAKG"
     gene            complement(6221..7447)
                     /gene="tyrS"
                     /locus_tag="VFR20_09345"
     CDS             complement(6221..7447)
                     /gene="tyrS"
                     /locus_tag="VFR20_09345"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU0230764.1"
                     /translation="MIPAEAPVTPEVETGLRVAKRGCDELLVESEFVRKLARSRATGV
                     PLRIKLGLDPTAPDIHLGHTVVLNKMRQLQDLGHRVIFLIGDFTSMIGDPSGRNVTRP
                     PLTAEQIRANAETYYAQASLVLDPERTEIRYNSEWSDPLGARGMIQLASRYTVARMME
                     REDFTRRFKGGIPISVHEFLYPLMQGYDSVALQADLELGGTDQKFNLLVGRELQKEYG
                     QEPQCILTMPLLEGTDGVDKMSKSKGNYIGISEAPDSMFGKLMSISDTLMWRYFELLS
                     FRSLDEISLLQKQVKAGANPRDTKVLLAQEIVARFHSQQAAHDALARFEARFRDGVIP
                     EEMPEVRMGRAPLGILKILREAGLATSGAEAQRNVEQGGVRVNGERIDDKGLLLDAGT
                     YVVQVGKRKFARVTLS"
     gene            7819..9111
                     /locus_tag="VFR20_09350"
     CDS             7819..9111
                     /locus_tag="VFR20_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEU0230765.1"
                     /translation="MLVVALGLFVTAAALAVVNPDSHDALPNLYQARAELALPDPFPQ
                     PSQHFSSPFVHQTRIRRGDTLAALLSRLDVREPGLLKFLTHDKDARSIYKLYPGRLME
                     AGVDENGALSWLRYDHTPGAEENGQYVAKWLEVRPDEKGGFTAVERTEDADVRMRVAE
                     GEIHSSLFGATDAAGIPDAITLQMANILDSKIDFIKDLRKGDRFQIVYQSYSHNGQDV
                     GAGRILALEFVNDSHTYQAVWFQPKNGTGGYYDFSGRSLKGAFLRTAIAFTRISSTFG
                     MRMHPLHKKWTKHEGVDYAAPSGTPIHATADGVVKFIGQQRGYGNIIILQHPHDYSTV
                     YAHQSRFASGLRKGDHVKQGQVIGYVGATGWATGPHLHYEFRIDNKPVNPLSASLPVA
                     FKLDGGELAAFQRTTSSYKGQIAMLADLQDHHIQVASR"
     gene            9124..10230
                     /locus_tag="VFR20_09355"
     CDS             9124..10230
                     /locus_tag="VFR20_09355"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEU0230766.1"
                     /translation="MTHMLYAGLMSGTSTDGIDGVLADFPPVGPPRIMAQVSMPMPET
                     LRREFLALNSPGDNELERAALAANALAKLYADAIGQLLARAKVGAPDVAAIGAHGQTV
                     RHRPEAGYTIQLNAPAVLAELTGIDVVADFRSRDVAAGGQGAPLVPAFHQALFQCDHP
                     RAVLNLGGIANVTLLAPNEPVRGFDTGPANVLMDMWCQEKTGATYDADGKWAATGHAG
                     EELLDVMVESEPWFRLPPPKSTGRDRFNRQWLARRLAIPEARGMRDNDIQATLRNLTA
                     RTVADAIAAHAPQTQDIVVCGGGALNDGLMATLTHYLNRPVVSSAELGVPVQMVEALA
                     FAWLAWAHGAGHAAGLPSVTGARHATPLGARYPA"
     gene            complement(10296..10664)
                     /gene="erpA"
                     /locus_tag="VFR20_09360"
     CDS             complement(10296..10664)
                     /gene="erpA"
                     /locus_tag="VFR20_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEU0230767.1"
                     /translation="MNALIETVDLQAPPSPLVFTDAAVAKVKDLLAEEDNPELNLRVF
                     VQGGGCSGFQYGFTFDETTNDDDTVVDKDGVKLLIDPMSFQYLVGAEIDYKDDLEGAQ
                     FVIRNPNAVSTCGCGSSFSA"
     gene            complement(10714..11439)
                     /locus_tag="VFR20_09365"
     CDS             complement(10714..11439)
                     /locus_tag="VFR20_09365"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HEU0230768.1"
                     /translation="MTPKPTHIPGSAVKPPDAARSRWPGLLLALLIGLVVGFFASRAW
                     LANRGLDRYDQLKARFDQLSTQSRADADRLQAQADKLQGELDVELGARKGLEASLATA
                     QRELGRTRDQLAFFRQLFPPGPSGSVNIRALEVRQQGPALAYKVLLSRNAAPGSLFKG
                     SLRFVAQGQEKGKTVKITLMPLRVAQPATPANTDELALSFDQFQRSEGKLALPTGFTP
                     QSVMLSVLEGNAVRVTRTVKLSP"
     gene            complement(11491..12555)
                     /gene="argC"
                     /locus_tag="VFR20_09370"
     CDS             complement(11491..12555)
                     /gene="argC"
                     /locus_tag="VFR20_09370"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEU0230769.1"
                     /translation="MSQASSSRIKVGIVGGTGYTGVELLRLLSVHPDVELKAITSRSE
                     DGMPVAEMFPNLRGLVDLSYQTPDAASLSDCDVVFFATPHGVAMDQARELLRHDVRII
                     DLAADFRLHDTRAFEKWYKMPHACPDILADAVYGLAEVNRDKIAKARVVGNPGCYPTT
                     VILGLLPLLEQGRHLIDPAHIVADCKSGTSGAGRKAAVGNLYSEAGDNFKAYAVKGHR
                     HHAEISEQLGGLVGGPVGVLFVPHLVPMIRGMFSTIYAPLLPGARGTDFQALFERRYA
                     NEAFIDVMPAGSLPETRSVRASNYLRIALHQPEGADHLVILVVQDNLVKGAAGQAVQN
                     MNIMFGLPEQAGLRHVAVLP"
     gene            complement(12761..13153)
                     /gene="rpsI"
                     /locus_tag="VFR20_09375"
     CDS             complement(12761..13153)
                     /gene="rpsI"
                     /locus_tag="VFR20_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU0230770.1"
                     /translation="MIGNWNYGTGRRKTSVARVFLKKGSGKIVVNGKPVDEYFARETG
                     RMVVRQPLSLTGHLESFDFHINVHGGGESGQAGAVRHGITRALIDYDETLKSPLKQAG
                     LVTRDAREVERKKVGFHKARRRKQFSKR"
     gene            complement(13163..13591)
                     /gene="rplM"
                     /locus_tag="VFR20_09380"
     CDS             complement(13163..13591)
                     /gene="rplM"
                     /locus_tag="VFR20_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU0230771.1"
                     /translation="MKTFVAKPHEVKRDWYVIDAKGKVLGRVASEVARRLRGKHKPEF
                     TPHVDTGDYIVIINAADIVVTGNKSLDKKYYRHSGYPGGVYETNFQKMQQRFPGRALQ
                     KAVKGMLPKGPLGYAMAKKLKVYAGAEHPHSAQQPKPLEF"
     gene            14196..15350
                     /locus_tag="VFR20_09385"
     CDS             14196..15350
                     /locus_tag="VFR20_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0230772.1"
                     /translation="MHSTRKILLASLIAAGSLSVAHAGDINIGMAEALSGGAAQYGHS
                     IKNGFELAASEINAAGGINGDKIKFVVEDEQGKKDEAINVFKKLIFQDKVLMIFGPTL
                     SNSAQAADPIAQASKTVAFGTSNTANGITSIGNYIFRNSVTEADVLPVTLAVAQRKLG
                     IKKVAVLYGNDDVFTTSGYDNFKKALADLKIPVTATETFSKGDVDFKAQLTKIESTHP
                     DAIVLSALLAEGAPIMVQARQLGIKVPFIGGNGMNSVKIFTLAPGAASNNLWVGSPWS
                     IDNHTPENDKFVAAYKAKFHSAPDQFAAQSYDATHIVAQALKHIKLTGNLAADRTALR
                     NALPSVKWTGATGPFEFVQAKNKAGKPAGYDAKQTAIISVTDNGKYTIEK"
     gene            15390..16271
                     /locus_tag="VFR20_09390"
     CDS             15390..16271
                     /locus_tag="VFR20_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0230773.1"
                     /translation="MLEQQLINALSLGCVYALFALGFTLVFGVLGVINLSHGAVFMAG
                     AYAALYAIATLGLPLWAGLIVSFLAAGALGAIIDWLVLRPLRHRGAPHLIPMIATIGI
                     GIILTNGAQGIFGPNNLRFPTGTIPNQSFMLGGLHMTVIEVGIIFLSFALMLALMYVM
                     RRTRFGRALRAIAESPKAAWLLGINVERLFLTTSFVAGGLGGIAGVLIGLYSNALYPL
                     MGEPILRKGIAVVILGGLGSIPGAMIGGLFLGFSEVLSVAFIGSTTRDAVAFGLLFLV
                     LLLRPQGLFGKAVQRKA"
     gene            16278..17144
                     /locus_tag="VFR20_09395"
     CDS             16278..17144
                     /locus_tag="VFR20_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0230774.1"
                     /translation="MGSLATFWAIYGNVVMSLGTNALLALSIWLTLVCGMLSIANAAF
                     MGIGAYAAAICTMSLGMPFPVSLAAGMVAPAILAAIIGLPTIRLSGVYLAMATLAFGE
                     VVRVIVLNTQSITGGALGLNGIPQLTQWWHVLLAVIIVLLVLWRLKVSKVGRAFDAIR
                     GDETAASLMGVNVRSNKLLAFVLGAAIAGLAGALDAHLTFYIGPNEYGFERAVEILTM
                     TILGGIGGLVGPVIGSSIITLLPEVLRGFNSFRLVANGLILVLIVLFLPRGIWDPARY
                     AHWLRKGSKRRA"
     gene            17137..17913
                     /locus_tag="VFR20_09400"
     CDS             17137..17913
                     /locus_tag="VFR20_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009277042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0230775.1"
                     /translation="MLELSSISIHFGGLHVLHDVNVKVPEGAIFGLIGPNGAGKTTVF
                     NLITGLLSPNGGDILFEGRSLIGQKPHRITRLGLARTFQNIRLFKEMTLLQNVVVGSY
                     RHMHYGVSDLLFGLPAFRRQETQARDRAMELLTWLKLDHKAHDLADNLSYGEQRRLEL
                     ARALATEPKLLLLDEPVAGMNTGERAELMHEIRAIRDRGYTILMIEHDMHFVMGLCDE
                     IAVLNFGKIIASGQPEAIRNNEEVIEAYLGRDDDDTEVHA"
     gene            17910..18620
                     /locus_tag="VFR20_09405"
     CDS             17910..18620
                     /locus_tag="VFR20_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0230776.1"
                     /translation="MSAALEVRNLTVHYGKIEAVRGIDLALEQGGITALVGANGAGKS
                     TTLLALSGLLPKLAGSVLFEGEDITRLAPHQVVARGIVQVPEGRAILTTMTVRENLEL
                     GAYRQGGGVALKDFDYVFALFPRLKERLEGLAGNLSGGEQQMLAIARALMAKPRLLLL
                     DEPSMGLAPIVVQEIFQALRAINADGLTLFLVEQNVRQALKISQQGYVLENGSMALTG
                     TGKELLGHPRVLQAYLGV"
     gene            complement(18709..20076)
                     /gene="pmbA"
                     /locus_tag="VFR20_09410"
     CDS             complement(18709..20076)
                     /gene="pmbA"
                     /locus_tag="VFR20_09410"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEU0230777.1"
                     /translation="MNQNAVSELPIAENQGRFRDLVRQALDEARALGASDAVAEVSES
                     RGLSVSVRKLALETVEQTRDRSLDVTVYAGQRRGSASTSDFSADALRQTVEAAWHIAR
                     YTAPDPAAGLPEAEFLAHDVSDLRLHHPWSLSAEEAAELALRVERASRAVSPLVTNTE
                     GASVGTLEGHFVMGNSRGFLGGYPYSRHSLSVAPIAGKGAAMQRDYWYSAARDPANLA
                     DPEAVGRYAAERTLARLSARRIPTGKFPVLFEAPLAVGLLGALVQAVSGGALYRKASF
                     LLDSLGKPVMSGHVDIDEDPHIVGAMGSAPFDDEGVRTQPRRLVDAGVLQGYFLSSYT
                     ARKLGMASTGNAGGSHNLVLSSRLTRPKDDFRAMLRKLGTGLLVTELMGQGVNYVTGD
                     YSRGAFGYWVENGVIMHAVQEITIAGHLADMFQRIVAVGADTITRGSKTTGSILIEQM
                     AVAGT"
     gene            20250..20864
                     /gene="yjgA"
                     /locus_tag="VFR20_09415"
     CDS             20250..20864
                     /gene="yjgA"
                     /locus_tag="VFR20_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HEU0230778.1"
                     /translation="MPHNDTAPLDAPSEPTDDDKPSKSQVKRDMHALLDLGKQLVDLP
                     VDRLRQLDLAEPLYDAILLAQRTHSREGRRRQIHYVGKLMRKAPAEAIRVQLDTWANG
                     SREETRSMHRLEALRDLLLNDDDALTQLLQDYPGADVQQLRALIRAGRRERQQNQTLQ
                     AGQEPIRKHYRALFQALKALSAGTPADTSATAQPDTPSTEAPAS"
     gene            20903..21577
                     /locus_tag="VFR20_09420"
     CDS             20903..21577
                     /locus_tag="VFR20_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0230779.1"
                     /translation="MNTNTLSDRIEIETAPNPTHAIIWLHGLGADGNDFAPIVPELRL
                     SSNPRLRFVFPHAPVRPVSINGGMPMRAWYDILTPDLVRREDDQGIRDSEALVRTLIE
                     RENARGISTENLVLAGFSQGCAMALHTGLRLDHPLAGIMGLSGYLPLADTVTAERHTA
                     NQNTPIFLAHGTLDPVVVLPRAQASHQRLTDMGYDVHWKTYPMPHSVCPQEIADIADF
                     LNTVLK"
     gene            complement(21676..24150)
                     /locus_tag="VFR20_09425"
     CDS             complement(21676..24150)
                     /locus_tag="VFR20_09425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0230780.1"
                     /translation="MLRHRLAALFEPCSLLVLADRDLPIAHAAPSALAGRVEFVEVGG
                     DGAMVVPPRPAGVAEGDRLDAALVCVASVHIPRALEALAPLRPRAVVLLRHPETTTDP
                     IEDMAYCQTWARLHDCELLGPHSTGVQRPVYGLNLSHETRPALVGRVALVTQSQSLTG
                     AVLDWAEDIRLGFSTAISLGDEAVIDVAAVLDYLAMDAQTDSIVLYLENTASSRRFGS
                     ALRAAASIKPVVVLKTGHGRFDPASSEDAVFNALLRRVGAVRVRYFVQLFSALKVLTY
                     AQRPRGRRVALFSNGHGAAQLAIDAMGPGAKVFCAELSRATATELGEMLDARAQVENP
                     VVTHAPLDPGTVDRALRVLVRDPGVDGVLVLLTPDPLADIAGVVSVLARLSREARKPV
                     VVSLLGDAGMRPLRHVLDEVGAPAFRTPETAANAFGILASYHYNQELARQILPPEPLG
                     LPVRSDRARAVLEGVRADGRRDLTPSECQDLFACFHVPITLRSGPGEDDGHGENTVPM
                     AIRVRRDPRFGPFVVFGVGGSQAVLSAGDEGVELPPLNGHLARQLVRRSALWRRVLSR
                     ELGVAACESLQEALERISDMVCDLPDMEDLEIDMLFAHGARLLTSRVTLSLTSAIESA
                     APQTTGYRHMAIHPYPTRLVQARVFKDGAPWTMRPIRPEDAEALQVFVRGLSDESRYM
                     RFVSMLRELTPAMLARYTRIDYDRELALVAAVQVPNPEHRGHPRDEIVGFAHYLRNAD
                     GRGAEYALVIADAWQHHGLGHVLMRGLITAAREQGLAYIDGVVLTGNRPMLGLMTHLG
                     FKNDPDEEDPGMRRVWLDLGEGAVAS"
     gene            24233..25660
                     /gene="gltX"
                     /locus_tag="VFR20_09430"
     CDS             24233..25660
                     /gene="gltX"
                     /locus_tag="VFR20_09430"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU0230781.1"
                     /translation="MTQAVPAATPAVRTRFAPSPTGFLHLGGARTALFSWAYARHHHG
                     TFVLRVEDTDLERSTPEAVQAILDAMAWLDMRPDEGPFYQMQRMDRYREVVATMLKAG
                     TAYHCYSSPEEVEAMREKARAAGLKPRYDGTWRPEPGKTLPPVPAGRKPVVRFKSPQT
                     GTTGWDDLIKGPISFDNTELDDLVIARPDGTPTYNFCVVVDDWDMKITHVIRGDDHVN
                     NTPRQITILRALGATPPQYGHVPMILGPDGEKLSKRHGAVNVMQYDRDGYLPEAMINY
                     LARLGWSHGDDEIFSREQLVEWFDTRHLSKSAAQWDPKKLNWVNAHYIKALGDADLAA
                     RVAPRIRELGGDPDAVDLQAVVALLKDRAETLNQLADGAMLFCGPYQPAPEELRGQHL
                     DADAVGLLREFARRAAGLPDWSAAPLSALIKAMLEEHGVKMPKLAIPLRVAVTGRKQT
                     PAIDAVLTILGRATVLARLDALSDQ"
     gene            complement(25682..26179)
                     /locus_tag="VFR20_09435"
     CDS             complement(25682..26179)
                     /locus_tag="VFR20_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230782.1"
                     /translation="MRTAMFHVGLFAVAAGLGIVLAGCSSPKPRPGPKPAAPAQVVCR
                     AAQPGDPAVGTWLSEFHRRGVAGKYRVLFGLSADGTMRYEDQIKRAGTPPQGLEESGC
                     WSRDGGVLVLRTTKSNGVPVEPGDPIYTNRYTVVSQSARTLTLRGENGLVKATRMSPG
                     YRLPY"
     gene            26415..26855
                     /locus_tag="VFR20_09440"
     CDS             26415..26855
                     /locus_tag="VFR20_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230783.1"
                     /translation="MSHSVLITLHLFAAIIFVGMVFFEVLILEGIRGQVPRDAMRSVE
                     IAIGKRARRFMPFVILVLYGAGIGMAWQFRGALAHPFASSFGTLLSLKILLALSVLVH
                     FITAMTLSAKGKLKTRHFQRIHVSVFLHMIGIVFLAKAMFYLHW"
     gene            complement(26938..27396)
                     /locus_tag="VFR20_09445"
     CDS             complement(26938..27396)
                     /locus_tag="VFR20_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEU0230784.1"
                     /translation="MDKTDLKILQYLQKNGRASAQQVADAVGLSAAPVWRRVKSLESS
                     GAILGYNARVSRSHVGLRGCMFTQISLERHSAEIVDNFIRAVRDAPEILECYTVTGDA
                     DFLLKVLVESAEAYDDFLHRFLFNLPGVRQTRTIVALREIKHEVELPLAL"
     gene            27528..31022
                     /locus_tag="VFR20_09450"
     CDS             27528..31022
                     /locus_tag="VFR20_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEU0230785.1"
                     /translation="MPGTTLDTRYQLTDNLTATSGRIYLTGPQALVRMALSQRRIDAA
                     RGLNTAGFVSGYRGSPLAGVDMAMWRAQALLEEAHVRFLPAVNEDMAATMMMGTQQTG
                     ERADRTVEGVFGMWYGKGPGVDRAGDAIHHGNAAGASRHGGVLMIVGDDHTAASSSIG
                     HASETSLTAWSIPIINPSSIEEYEYFGLWGWALSRFCGAWVAFKAITETVESGRSFEL
                     QSIPDFSACLNDPRAGKLQYSAREFLTPAMEHRIAERLTAVQAFSQLHRLDRLVNPAP
                     QGALGLVTTGKATLDTEEALRRLARSGAHLPPIRHYKVGLTWPLDRAGLDAFVDGLEH
                     ILVVEEKAPIVENQIKELLFNRARRPTVQGKTDLRGQPLVTGEGQLNPTLVATALRGW
                     LRDAGALTLPEPTGRAPVLDPATTHGLARRPYFCSGCPHSTSTKVPEGSQALAGVGCH
                     YMATWMDRDTSGLTQMGGEGVDWIGLAPFTRTPHVFQNMGEGTYFHSGYLAIRQAVAA
                     QAHITYKILFNDAVAMTGGQPVDGHLSVPDICRQMLGEGVARVVITTDEPQKYQGVPL
                     VQGVAVHDRHELDSVQRELRDIPGVTVLIHDQACAAEKRRRRKKKAFPDPPRRLFINT
                     AVCEGCGDCGTQSNCLSLVPVETPFGRKRAIDQSSCNKDYSCVDGFCPSFVSVMGGTP
                     RRSARIAEPGNQARLETLRRQLPDPVILAGGEVCNILVAGIGGSGVVTLGALLAMAAH
                     LEGRAVSTLDVTGLAQKGGAVISHVRLARGAQARGVVRIDQRQADVAILCDPVAAARP
                     DIVATLKPSATLVTLSTYLAPTAEFTRDPDTNLDASRLVAGLRDIAGAANTRALDAHE
                     LALSLMGDAQLSNVMMLGFTWQRGAIPLDRASVERAITLNGVAVPANLQAFELGRIAA
                     WQPDTLDKMLHPSRQSRVIPFPESLDTVVERCRDDLTGYQSAAYAKTYTDTVAAVARA
                     EQALHPQAKPVLALAVARSLHKLMAYKDEYEVARLFSDGRFRRELQSRFEGDFTLRFH
                     LAPPLLARRDPRTGAPRKMTFGPAMEAVFRVLAHGRRLRGTWLDVFGYSAERRTERRL
                     ARQYRARILDMLGKLNERTFPIAVELAALPDMVRGFGHVKQASIDRYDQRLAEMTRTL
                     SAEPGHPAHTPSVVRRAG"
     gene            31343..34252
                     /locus_tag="VFR20_09455"
     CDS             31343..34252
                     /locus_tag="VFR20_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEU0230786.1"
                     /translation="MQTTQNAESGRAATPAPALDPVKDASSTNQWHNFNIIRRNGAVV
                     GFEPSKIAIAMTKAFLAVNGGQGAASARVRELVETLTARVVNSLIRNRPSGGNFHIEE
                     IQDQVELALMRSGEHDVARAYVLYREKRSQERARDTQAQAPAAQSTLNVTDNGIRRPL
                     DLALLRATIADAGQDLPQAIDADRILKETVKNLYDGIPVDEVFKSAILSARALIETEP
                     SYSQVAARLLLHTIRKEVLGEEVSQAAMAERYADYFPRFIKLGIELELLDEKLAQYDL
                     DKLAVALTSSRDQQFTYLGLQTLYDRYFLHDAHGRRIELPQIFFMRVAMGLALNEIDR
                     EARAIEFYEVLSSFDFMSSTPTLFNSGTLHSQLSSCYLTTVSDDLVGIYDAIKENALL
                     AKYAGGLGNDWTPVRALRSHIKGTNGESQGVVPFLKVVNDTAVAVNQGGKRKGAVCAY
                     LETWHLDIEEFLDLRKNTGDERRRTHDMNTANWIPDLFMKRVMEEGDWTLFSPSDVPD
                     LHDKVGRAFEAAYVGYEEKAARGELTLFKKLPATSLWRKMLSMLFETGHPWITFKDPC
                     NIRSPQQHVGVVHSSNLCTEITLNTNDSEIAVCNLGSVNLAAHLKQEADGRYVLDHAK
                     LERTITTAMRMLDNVIDINYYAVPKARNSNVRHRPVGLGMMGFQDCLHIMRVPFGSDA
                     AVEFSDRSAEAVCHYAYWASSKLAVERGHYASFKGSLWDRGILPQDSLALLREERGGY
                     VDIDDSSTLDWDTLRTHIKQYGMRNSNCVAIAPTATIANIVGVSPCIEPTYRNLYVKS
                     NLSGEFTVVNDYLVHDLKERNLWDEVMVADLKYFDGSLERIDRIPADLRTLYATAFEV
                     DPRWIVECAARRQKWIDQAQSLNIFMRGVSGKKLDDTYKLAWVRGLKTTYYLRSSSAT
                     NAEKSTGRGGELNAVSSGQNPGVSAAANLPEPEILGAVCTLRPGDVGFEDCEACQ"
     gene            34281..35444
                     /locus_tag="VFR20_09460"
     CDS             34281..35444
                     /locus_tag="VFR20_09460"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEU0230787.1"
                     /translation="MLNWDDTPLQSSTPAPAPARAERPAAPVGAASDTPIPTPAAAST
                     PSPDAHRVRASEKRIINGKTDVNQLVPFKYKWAWEKYLATCANHWMPTEINMSRDIAL
                     WKNPEGLTDDERRIVKRNLGFFVTADSLAANNIVLGTYRHITAPEARQFLLRQAFEEA
                     IHTHAYQYIVESLDLDESEIFNAYNEIPSIRAKDEFLIPFINAIADMNFKTGTPEADQ
                     TLLRSLIVFACLMEGLFFYVGFTQILALGRQNKMTGAAEQYMYILRDESMHCNFGIDL
                     INTIKLENPHLWTPEFREEIRGLFKKAVELEYAYAEDTMPRGVLGLNASMFKSYLRFI
                     ANRRCQQIGLEALFPQEENPFPWMAEMIDLKKERNFFETRVIEYQTGGALSWE"
     gene            35613..36332
                     /locus_tag="VFR20_09465"
     CDS             35613..36332
                     /locus_tag="VFR20_09465"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEU0230788.1"
                     /translation="MLTQGTWQGALGTAALSVIIFSTSILLSLVSGFFGWYSILIGIF
                     LFIGFYVIITILPLLLNLSRSFYWIIFALLIIHISITILSFALNYHSTGLLGSKGKFA
                     PSFYDSLYFSITTFTTLGYGDIRPLPSYRLTTSLEALIGMVSMALGASVIWLWCQENL
                     IPKEIAYFDGNRRHKTSLGITRLRIRTMTGKEHNLTDWYLPAKPGETYRYDDLRQEWI
                     STPEKIEPLEDAAIMSIDHRD"
     gene            complement(36731..37264)
                     /locus_tag="VFR20_09470"
     CDS             complement(36731..37264)
                     /locus_tag="VFR20_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEU0230789.1"
                     /translation="MIMANPYDSIAKVPSFTLTSTDIADGVRLKSAQVSGIFGAGGTD
                     TSPQLSWLGFPPGTKSFAVTVYDPDAPTASGFWHWAVADIPVSITQLASGAGDERSSG
                     LPPGAWQLANDANLKRYLGAAPPAGHGKHTYYTAIHALDVAQIGIDRESTPAFLMFNI
                     RPHTLARAVLAPWWEVS"
     gene            complement(37292..38320)
                     /locus_tag="VFR20_09475"
     CDS             complement(37292..38320)
                     /locus_tag="VFR20_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007741897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEU0230790.1"
                     /translation="MKAVRLHRYKEQPSIDDVDEPTVKGPWDVIVKVGAAGLCRTDLH
                     IIEGQWEAIQHPRLPYILGHENAGWVSAVGSAVSNVAVGDTVIMHPLASCGLCPACRV
                     GDDSHCLHSDFPGLNVDGGMAEYMSTNARAVVKLDSSLQPRDIAALADAGITAYHAVR
                     KAKNLLWPGSHAVVIGAGGLGHIGIQALKAITAAEITVIDRSEAALELARELGADHTV
                     VSIDDDAVTKQVLDITDGGAHVVFDYVAEHGTEALAPKILRNSGSYYVIGYGGMIKLP
                     TIDFISREISVVGNLVGTYNDLVELMTLTARGRVKLHTNVYPLEAACDALNDLDQGRM
                     VGRGILVP"
     gene            complement(38414..40000)
                     /locus_tag="VFR20_09480"
     CDS             complement(38414..40000)
                     /locus_tag="VFR20_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEU0230791.1"
                     /translation="MAKYDNQKTFSHDDDHVVVIIGSGAGGGTLANELAQKGIDVVLL
                     EAGKLHTSAEFISDEWPSFHQLAWLDKRVASGGWRIAKDFPNLPAWICKTVGGTSVHW
                     AGASLRFQKHEFKARTTYGALPGTTLLDWPITLEEMEPYYKKAEWKMRVTRTNNLPGL
                     PGNNNFKVMYDGATKVGYKECSTGRMAINSIATDGRSFCHQRGFCFQGCLYEAKWSTL
                     FTEIPRALATGRVELRAEAMVARIEHDNKGRVTSVVYFDKDGHEQRQKARVVAVAGNS
                     IESPRLLLNSHSARFPQGLANGSGQVGRNYMSHLTGSVFGVFEKPVDMYKGTVMAGIV
                     EDEKVNDSKRGFSGGYHFETIALGLPFTAAFLNPGAWGQEFAEAMDNYTHMAGMWIVG
                     EDMPQANNRITLHATEKDQHGLPIPVVSYTDHANDKAMREHAYKRGEAIYQAAGAKRV
                     YRVPPYPSTHNMGTCRMSAKADDGVCNAHGQAHEVRNLFISDGSQYTTSAAENPTLTI
                     VTLAIRQADYIAGQMSRREL"
     gene            complement(40027..40491)
                     /locus_tag="VFR20_09485"
     CDS             complement(40027..40491)
                     /locus_tag="VFR20_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tat (twin-arginine translocation) pathway signal
                     sequence"
                     /protein_id="HEU0230792.1"
                     /translation="MLAPTKTWALELDTLDSHQGKTLLRFTRYLYPHKTLEDAVYALV
                     VKNLDEDAAKDQSLRKLLADGVANMDKTAKGNWLSADGARQFAEVKALAGTPFFEKVR
                     STAVVALYSNELAYAHFGYPGPKGATGYLHRGFNDLKWLVSPPASASGPVPN"
     gene            complement(40910..41191)
                     /locus_tag="VFR20_09490"
     CDS             complement(40910..41191)
                     /locus_tag="VFR20_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEU0230793.1"
                     /translation="MCQISKSIGSNFFERETRSVRIKGYVTSMGLEEEFWVILKELAE
                     DQGMSVGLLLAVLYGEYMQSGCASNFASFLRVSCVEVVRGQRLVPERIE"
     gene            complement(41619..42551)
                     /locus_tag="VFR20_09495"
     CDS             complement(41619..42551)
                     /locus_tag="VFR20_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0230794.1"
                     /translation="MLDGVSLDQLRAFIAAVDEGSFSAAGRKLYRVQSAVSGWIGGLE
                     AQLDVVLFDRTARFPKLTPEGLLLLADARNIVSGVDAMKARAKLMAGGLEAELSVVVD
                     VFFPATTIAAAAKAFAERFPLTPLRLFVEGLGAAYQPVLDERCSLGILASLPVEFPSL
                     VSEPVNEVPLVMVAAPSHPLAGFRGRIPKRELAKQVQLVLTDRSDLMAGRDYGVFSPS
                     TWRLADLSTKYAFLKDGVGWGSMPRHMVDSDVAAGSLVLIDVRADFTLTMSACYRASA
                     PPGPAGRWLIDYLKDLSSEPSTTDKPHPLLPTDN"
     gene            42696..43310
                     /locus_tag="VFR20_09500"
     CDS             42696..43310
                     /locus_tag="VFR20_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU0230795.1"
                     /translation="MNTQYSIIGSGPVGRTLAGLFTRGGIDVQLANSRGPESLAAIAA
                     ELGRHVVPVTLERALDADVVFLAVGFMQVQSVAMKRPDWAGKIVVDVTNALQPAEVRE
                     TQLRGLLSSEINAGFVSGAKLVKAFNHLPVQQLGTNPSLSGQRQVVFVASDDAGASTA
                     IAALATRLGFAPVELGRLDQGGAAVHVLDGKRGGLLFQNLVKLG"
     gene            43360..44154
                     /locus_tag="VFR20_09505"
     CDS             43360..44154
                     /locus_tag="VFR20_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0230796.1"
                     /translation="MIIDLKGRKAIVTGSTAGIGRAVAEGLARAGAAVVINGRSEERV
                     FAALREMRTLFPAADITGFAADLATAEGAATFSARATDADILVNNLGTARPKPFLELT
                     DSDWLDMFEINVMSGIRMTRHYLPGMVSRGWGRVVFISSESALAIPKDMIDYGMTKTA
                     QLAISRGVAESVAETGVTVNAIIPGPTRSEILSNWMKAQAESEGITQEEAEQNFVRTM
                     RPTSLLQRFTTTDEVANMVVYVCSEQASGTTGAALRVDGGVLRQIA"
     gene            complement(44379..44546)
                     /locus_tag="VFR20_09510"
     CDS             complement(44379..44546)
                     /locus_tag="VFR20_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230797.1"
                     /translation="MGYPLVFSSGANIHVGYGLFMFVPFALFYMWAARHERHPEEHGL
                     FAPRRPAIEEA"
     gene            complement(44554..45540)
                     /locus_tag="VFR20_09515"
     CDS             complement(44554..45540)
                     /locus_tag="VFR20_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU0230798.1"
                     /translation="MSKITALAACLIWAMAGTAAPAHAAPTDQVNRAAVTPPWQNGAN
                     NDATHRGFEFTVPDADNLADFHGDPVHPVLSLYVGGNYFFAMSPLVRAFEHKYPEYKG
                     RLYWETIPPGLLVRQIKAGGTVTVGNMTWTVKPDVYLAGLKAVDKQITAGLLDAPAMP
                     YVTNTLTIMVPKGNPAHITGLADLGKPDIRLAMPNPKFEGIARQVEASLVKAGGQSLK
                     TMVYQTKVAQGTTELTHIHHRQTPLWIMQGKVQGGVTWQSEAMFQEQAGHPISHVDIP
                     TAQNTTAIYAGALVKGTPHAQAAHRWLDFIRSPEGLAIFKRYGFKPYVASVK"
     gene            complement(45591..46145)
                     /locus_tag="VFR20_09520"
     CDS             complement(45591..46145)
                     /locus_tag="VFR20_09520"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEU0230799.1"
                     /translation="MHKLTALLAAGVLLATSVASHAQSPGAFWTTPTIQGYGRIHMVS
                     HAAYRPQANQTYKIVFALTQAAKTPTQVNPALDHVARTVNLYVASGVPLDHLKFVAVA
                     YGAATPLALDNKDYRAVYGVDNPNLPLIAKLRKAGVDVAVCAQAVAEHKFHYGWVDHS
                     VTLALSGLTTVTTLEHRGYDLMEL"
     gene            46307..47212
                     /locus_tag="VFR20_09525"
     CDS             46307..47212
                     /locus_tag="VFR20_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0230800.1"
                     /translation="MHMNLHHLSVFHTIAITGSITASSQQLHISQPALSHELKNLEDR
                     LGVALFERLPRGMRLTHAGEVLRDYADRLFEIARTAESEMRAIAEARKGALTLGASNT
                     IGTYVLPRLLAHFRRDNPDVRISLFVGNTAQISQGVADMRFALGFIEGPLHTDGLVAE
                     RFQDDELLPVVAAGHPLLSRKRLGIDSLDGEPLLVREAGSGTRELLMDMLQVHQVKLG
                     SMMEFGNTEAIKQAAINGGGIAWLPRISMARELESGVLFTLPVKSLVINRPFRVIRRP
                     RAHSSAVIDSFLALLSLQKQPKKSD"
     gene            complement(47306..47653)
                     /locus_tag="VFR20_09530"
     CDS             complement(47306..47653)
                     /locus_tag="VFR20_09530"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEU0230801.1"
                     /translation="MMANHDTQVVEPAADERQELFTYITGLIAALILTLAAFALIHYW
                     PSLPQHTLFMAIGALALLQAAVHFRFFLHIGTKHSGDEFKVLLFTILMLIVMVGGTLW
                     VMSNLAMRMMPHF"
     gene            complement(47650..48273)
                     /locus_tag="VFR20_09535"
     CDS             complement(47650..48273)
                     /locus_tag="VFR20_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEU0230802.1"
                     /translation="MADNVFQYPGMRMATEPEEEHDESSTIMFGFWFFLMSDAVLFAL
                     VFSTYLFSRHATSGGPGPRTLYDMGSVFAQTLLLLASSFTFGLASLALKYKHSLTRLL
                     GWMLVTLVLGAGFLALELHDFFSMVALGGAAQNSGFLSSFWDLVPLHGLHVFVGCVWM
                     VCIIAQIWRYGLDHWSRLGIQRLALFWHFLDIVWIGIFTIVYIAGVA"
     gene            complement(48295..50466)
                     /locus_tag="VFR20_09540"
     CDS             complement(48295..50466)
                     /locus_tag="VFR20_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEU0230803.1"
                     /translation="MDAAAIFSHSIGRLGASSLPFYEMFHNPTTVNVVNGGIATFAAG
                     CVVFGAVAAIALISYHHKWSELWEWLTSVDHKKIGIMYVVLALVMLARGLLEAGLMRG
                     QQAWGLGGGFLTPGHFAELFSTHGTLMVFFMAMPFFVGVINYVMPLQIGARDVAYPTL
                     NAVSLSLTAAGAGLLMISLVFGQFSTGGWFGYPPYTETAWQPGVGPDYWIWALTLSGI
                     GSTMTGINFVVTILRKRAPGMRLMAMPLFSWTSLVTGIMLVFAMPPLSVAMGQLALDR
                     YLGLHYFTNAMGGNMMSFANLFWLFGHPEVYILIMPAFGIYSEVIPAFSGKTLYGYKS
                     MVFATACIAFASFLVWVHHFFTMGQGPDVNAVYGIASMLIGIPTGVKIYDWMATMFRG
                     RVRFTTPMLFSIQFIFLFVIGGMTGILLANPTVDYQVHNTVFLVAHFHNVIIPGVLFG
                     LVAGFEYWFPKVFGFRLNDRWGKASAWCWIIGFILAFFPLYALGLLGAPRRMVGYSQP
                     AYVPYETVAFVGAVLLNIAFFLLLAQVWVSWKQRAENQVFAGDPWDGRSLEWATSAPP
                     PEYNFPVVPVINSRDAFAVAKEAGLAYSTPQEFVDIEVPKNNALAVQIGAATFLLAFG
                     LVWHIWWLVIAAAIATVVFMVRFGFVHHMTRVIPASVVEKETLLWLAHVREAKPVTRA
                     EEFSPANVGVAALETGVNAAEAAGRRGASRLDLAPQAPRRS"
     gene            complement(50506..51543)
                     /locus_tag="VFR20_09545"
     CDS             complement(50506..51543)
                     /locus_tag="VFR20_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit II"
                     /protein_id="HEU0230804.1"
                     /translation="MNSTPQQSGVLDAAPKFHWQPGAWLALRIFSIIGSTFVLSSCAR
                     EPLRNLHDHLSFLDPQGPIAAAERWHFWFVVLIMTVLVAAPVYILTIWILWHYRYSNK
                     AHTRYEPKWTHNSVLSVLTWSGPVAIVMFLGVFVWRNTRRLDPFRPLVSTQPTLQVQA
                     IGYNWKWLFLYPKLKIATVGVLPIPAGQPVAMKLTSATVMQSLSIPALIGQIYAMGGM
                     VTQLHFEANGPGRSLGMNTMYNGSGFARQTFTALALKPTAFKAWVKQVQAYGVPLDQH
                     TYALLSQRGTKGQLASALRLPSSTVRDGEIFLRDVPTGLFHQVIHATTSGTTVKFPQM
                     NGVSANAPAIP"
     gene            complement(51967..52431)
                     /locus_tag="VFR20_09550"
     CDS             complement(51967..52431)
                     /locus_tag="VFR20_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0230805.1"
                     /translation="MSQRLNYVHASPDGFKAMRNLQQYVENSGLEHPLLELVKMRASQ
                     MNGCAYCLDMHSKDARAAGETEQRLYVLSAWREAPFYSERERAALAWTEALTKISDGP
                     VEDALYEQVRKQFNDKELTDLSFAIIAINGWNRLAIPFRSEPGHYQPGEHAA"
     gene            complement(52459..53508)
                     /locus_tag="VFR20_09555"
     CDS             complement(52459..53508)
                     /locus_tag="VFR20_09555"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEU0230806.1"
                     /translation="MAFFRHIRRAKTPFTASRLVQGFDFWVVNPVNMIYNGIVIGVWA
                     LTPATRNAGLRLAPDPSSLFAGVNFLEIFAMTTTTLSARHAAFRKLHETGCFILPNPW
                     DPGTARWLEQTGFKALASTSAGYAFSRGIPDREIPVDEVVRHCGELVRATGLPVNADF
                     EDGYSSTIDGLKVNVRACVQVGVSALSIEDSIAGSEGALYDFDEAVARMRAAREAIDS
                     VDKNVMLVGRAECYLVGRPDLDETIKRLKAYADAGADCLYAPGLSRREEISAVVEAVA
                     PKPINIMMGHAAKLGFKTLAEIGVRRISTASTLALAAWSGFVNATDRLMREEIDGFEN
                     TVDYLQLCERFKIYR"
     gene            53638..54828
                     /locus_tag="VFR20_09560"
     CDS             53638..54828
                     /locus_tag="VFR20_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0230807.1"
                     /translation="MCLLIGARAVRSVGQGALVAAFTLYLDAMGWSAAAIGAVLMSAL
                     LFSIVLTAIAGPLSDRGHRRTFLLGYDVVQAVVAIVALTTTTPWLLIGAAVIGGFGRG
                     GNGSAGPFSPIEQAWLAIELPMSRRGSVFSVNSAVGLIGMGIGALLAALPTLWIGHAM
                     STQDYRLLFLLPLAGALASFALLWAAREPNIVVRADRDEASLRTHRAENRLLAKLMLA
                     NTINGFAIGLTGPLIAYWFAIRFHRGLLEIGPGLAIAFLISAAGAFMAGALARRHGIM
                     ATVIWMRGLGVLFLFALPLVPWFWLAMLLYIARGAVNRGTGGVRQALAAGLTRVERRG
                     LASSLQNISLQLPRAIGPAIGGVLFHAGYLSLPFLIGAVLQLVYLILYARFFGSHPAA
                     QAHT"
     gene            54915..55862
                     /locus_tag="VFR20_09565"
     CDS             54915..55862
                     /locus_tag="VFR20_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEU0230808.1"
                     /translation="MPSTTHQPIDSPLTQAAMFLVLTINSEPGHLDAARAFCADLTGL
                     RRSVGFRDPDGNLSCIMGFGADAWDRLFGSPRPKALHPFKEIQGVHHAVSTPGDILLH
                     IRANRMDLCFELATQITARLGDAATVVDETHGFKYFDSRNLIGFVDGSENPTGVEATA
                     ATVIGDEDSAFAGGSYVIVQKYLHDLAGWNALPVEMQEKIIGRGKQSNIELDDDVKPT
                     WAHNALTVIEEDGEELDIVRGNMPFGDVGKGEFGTYFIGYARTPRRIEQMLENMFIGR
                     PQGNYDRLLDFSRAVTGSLFFVPSADFLDDVPEGGAPSP"
     gene            complement(55949..57445)
                     /locus_tag="VFR20_09570"
     CDS             complement(55949..57445)
                     /locus_tag="VFR20_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230809.1"
                     /translation="MKATHVGPIGLIALTALMPSAKAEQHQVSVTLVQTVKPTPHAYP
                     GVTADILGIRIGMTVAQAEAIAAKTYPGKPREIRTFLSVNHDGIFAKSQSFISQVAFQ
                     NGTRDSVTVYFSNPTTGNVVYGIYRGAIFEGAMKQPLITAVMSDLIKKYGPASSTFGY
                     EHWWQFGEQPLNPCNLQLCRRPGKNVVMGIYAQLNPGAGDHTKVMFLTMRMNDYQLRS
                     ESAAVGNSQLVTAAEAEKVTLAKQVVPAQAPTALTGIYVPIADTTPLVSELKENADNI
                     LDIAGAVTGRALVLECIGHVGATTSARAGSPILSFYALPKDVFQKYGLGPVTYNGKKY
                     DMSIQAYGKPPSTTNVDVRLDPVDGKGGSISSDNTLVGGSVAIATAEGAEAFTFDHAV
                     LKLKRSGAHGPSVPPNATVTVTGKAYCNGSHKAPLPQALRDKVPAGSLRFVSGTQVNV
                     LAREGEEPLTYKINGNHVQLTGPHGSVSIAIDSHGCLDAPEHTILCKK"
     gene            57719..58591
                     /locus_tag="VFR20_09575"
     CDS             57719..58591
                     /locus_tag="VFR20_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230810.1"
                     /translation="MKIKHDIVALVLAGVAGMATGTNAAAQCTTTPFPGPPKEVLIRC
                     TLAQTAQPIAPPTYPGVDFNIFGFRTGMTVAQVEALAAKIHLGKPGIKTKPLDNEVKM
                     WGATALAQGAPINQISYDFRLAGGSNRATVIIYFSSPATGSVSEAVYENSNYITDRRQ
                     KRPLVAPVVTKLVHQYGPSSSRTPSYMSQYLCWNFKGQSLVKASLRQPCYPNADDVMG
                     TNVAAMGNGTYHPADSYIYALLSTNSGEVNGLAQGLGDYRLAALDRSEIKKQLTLAIE
                     KYSPPTQTSASTHR"
     gene            complement(58709..59530)
                     /locus_tag="VFR20_09580"
     CDS             complement(58709..59530)
                     /locus_tag="VFR20_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230811.1"
                     /translation="MKSGTAVLSILVWGMSMTYAQASAYNAPMHSSSSSSGNSTSTGV
                     TVATSQDLKFSGDTCPFYPASYPKGTTYKVVTKIEESGGREYTEENDSEVLGPTTFRG
                     QKAIAIRENIELAYPSHKNVSIIDHFYGVGAQFVYLYGARVRSGGETYNDPPISLPRC
                     FVMNTPYTETDTMHTLATDGVEDRVTSQTTRVLLGIESVTVPAGTFQAYKVGSETSLG
                     SNGMKTGRTYSWVVASGRFAGFVIKNTFVRPASAQGSSTAFHMTRTALSVQLNGK"
     gene            59928..>60208
                     /locus_tag="VFR20_09585"
     CDS             59928..>60208
                     /locus_tag="VFR20_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230812.1"
                     /translation="MPKSTIRLLAAFLIGPCAPACAANPPVATIQLSGSKTLPYFISA
                     PPGPPGPIRALIGVQGFPRDANRTFDAIARATREAGKTATTLLVAPIFPV"
BASE COUNT        12352 a        19266 c        17308 g        11282 t
ORIGIN      
        1 gccagcgagg ccttcgccgc gaggacgatc cccgacgagc gggcgagatg ggcgaggaag
       61 cgtctacgtt gcatggtctg tcatccattg tttgcgtggc gaacgggccc ctccgcatca
      121 cacggtgaac ccggttttgc ccgaagctta aaccttccaa ctatggcaat gtcaagccat
      181 cgccgacatc gaccctcatc aatatcggac ctcaccaata tcgacttcca tcaatatcga
      241 ctctgattca cggccctgat cagtcccaag cggccagcct catgagtgtc gatgctgatg
      301 atgaatgtcg ggataatgtg gatggctgtg ggtgagcggc gcatgggtat gttcgtgcgt
      361 atgcggctcc cgcccattcc agtcgaaatc gtgcacatgc tggtgatgcg cgtcatgcaa
      421 gtgccgatgc gtgtgggtga tgggctcgtg cgtatgcagg tgttcatggc gctcagtcag
      481 atgcagccag accccggcac ccatcaccac cgccgcaagc cagaacgccc acgacgccgg
      541 atcgcccagc gcgaaaatgc cgatggccgc cccgatgaaa ggcgcggtcg agaaatacgc
      601 gcccgtgcgc gccgagccca gtccacgcaa ggcaagcacg aacaacacca gactcacgcc
      661 ataacccagc aaccccacca ccatcgccat gccgacgaac tgtggcgccg gccaccgcgt
      721 tcccatgaac acagcgatcg cgatgttcac cacaccagcc accagccctt tcaacccagc
      781 gatgaacacc gcgtcggatg ccgagacttt gcgggtgagg ttgttgtcca gcgcccagca
      841 caggcaggcg ccggcgatca gcaacgcccc ccaaccaaga tgcgcggcct gcgtcgcacc
      901 cggcaaagtc agcaacacag cacccgatac gatcatcagc atgccgatga cgatgcgacg
      961 gtccacgttc tccctgaaca cgacccacgc cagcaaggcc gtgaggaccg actccaggtt
     1021 caacaacagc gaagcggtcg ccgccgatgt gtgcgcaagc cccagcatca tggccagcgg
     1081 gccgagtatt cccccgaagg cgatcgcaag cagcaaccag agccattcaa tccgcgcgat
     1141 tcccgggggc cgccagcccc gatcccgtgc gattcgaacc acccccaggc caacgccact
     1201 gcccaggtac aacagccccg ccaacaggac gggcgacaaa tcatgcagca gcaccttggc
     1261 caacggcgtg ctgacaccaa acagcacggc ggcgccaagg gccgcgaata tggaggaatg
     1321 cgaaagaaca ggaatccgca tggttgccgg tgttacttcg ccgcctcggg agcgctgagg
     1381 ctcttgctgt aatggcccga ctgatcaaag cagcaaacgc gcttcttgcc tttggcaagc
     1441 atgtcgcatg cgttctccac ccggctcttc cgcgtcttgg cctgcttcgc ggactcgacc
     1501 caatgaaccc agtccacacg ggcaatggtt gtcgtggcct cccagacagc cttggcttcc
     1561 gggtgtgcgt caagggcctg cagcaaatca tccggcagca tgggttccgg ctctggatcc
     1621 gctggagaga tccgaacggt gaccgtatcc ccaaaatccg cacccgccgc ttcacgcagt
     1681 tcattgccta ccctcaacca gtggctaagc tgaccatccg gttcgagtgt cgcttggaag
     1741 ggatgcccgt tgatggtgcc ctgaacggag gttctgccac gcctcggcag ggtctcgctg
     1801 gcggatttgg gcagcacaag aaacgcccag gtgccgttgt tgccgggctc gaccggacgc
     1861 gatagcgttg ctttgaacgt gaatgagtct gctgttgcca tgcgggaagc ctttgacgcc
     1921 caacgtatcc tccagggcca tcccaagtat agtctgcccc gcgtgcgtat ccgccggcac
     1981 atcgctgtac acgtcaatgg aaccaaaaag cccgcaacgc catgaggcat gcgggctttt
     2041 gatatcctgg cggacaaggg gggattcgaa cccccgatac gttattcacg tatacacgct
     2101 ttccaggcgt gcgccttcaa ccactcggcc acctgtcctg cattcctgcg ccaaggcggg
     2161 atacgccacc gacgcgaagc ctgtaattct agcagaatcg gggggaatca ggcggaaggc
     2221 gcctgcaccg cttccagcag cgcctgccag cgatcagcac agcgagcgcg caagcgcatg
     2281 tccggtgcca gaaaaacact gtcgagaaac tggaaacggc gggcctgatc cagggatgcc
     2341 agagcgggca ggcgggccat gggttgggca tcacccagca acatgggcgc caggtacacc
     2401 aacagctcat ccacgcagcc ggcccccagc aacgcgccgc acagccccgg ccccgcttcg
     2461 acatggacct cgttgacctc ttgctcggcc atccagcgca gcatggcgtt cagatcaacc
     2521 ctcggccggc ttcccgcacc gtcatcggcc gtgcgcaaag cactgttatc ggccttggac
     2581 aaagccacgt catcgacctt cgaccacgtt acatcatcga ctatcgatgg tgcgccatca
     2641 tcgaccatcg gcaggaaaat ggccctggcc cccatggccg ccaggcgtgc cgctttctcc
     2701 gggtcgttcc ttgcggtgaa cacccagacc ggaccgcccc cgaacaggcg cgcgtcctcc
     2761 ggaatctcaa accggggatc gaccacagcg cgtatcggct ggcgcggggt ttcgacaagg
     2821 cgcacatcaa gacgggggtc atcggccagg atggtgccca atccggtcag gaccacgctg
     2881 ctgcgcgcac gccagtgatg gccgtcggcg cgcgcggtgg ggccggtgat ccactgtgag
     2941 acgccattgt tcagcgcgct gcggccgtcc aacgaggcgg ccagcttgag ccacacccag
     3001 ggcgttctgc gcgtcatgcg cgcgacgaaa ccagggttca gctcaagcgc ctcctcggtg
     3061 cagatcgaag tcgtgacggc gatgccgacg tcacgcagcc tgcccaggcc acggccgccc
     3121 accagggggt tgggatccag catggccacc accatgcggg ctggacgctc ggcgatgagc
     3181 gcctcgacac aaggcggcgt gcggccatgg tggctgcagg gttcgagcgt cacataaaca
     3241 gtcgccccct ccgcgctgag gtgatgccga tgaaggtcgc gcagcgccat gacttcagcg
     3301 tgcggcccgc cggcttcctg cgtggcgcct tcccccagga cttggccgtc gcgcacgatg
     3361 acacagccga cgcgcggatt gggcgccgtc aagtacatga cctggcgcgc caggtcgatg
     3421 gcccggcgca tccagtgctc atcatgccgg ctcatgatgc aaagggactc atgatggcgg
     3481 gggcaattgg atttcgtcaa tggccttgac gaattcgtcg atgtcttcga agcttttgta
     3541 caccgaggcg aagcgcacgt aagcgacctg gtcgagtctc ttgagttcgg ccatgaccag
     3601 ttcgccgatg tgcccggagc tgacttctcg cgacccgctg accaacaggc tttcttcgat
     3661 gcgggccacg gccgcgtcga ccgcggccgt atcgacgggg cgtttgcgca aggccagccg
     3721 caggctgccg cgcaatttgt cggcatcgaa ttcaacacgc gtgccgttgc gcttgaccac
     3781 cgccggcatg gtcagctcaa cccgttcgta cgtggtgaac cggcgctcgc aagccgcgca
     3841 gcggcgtcgg cgccggatgg tgtcgccttc ctcggaaacc cgggaatcga cgacttgtgt
     3901 atcgaaactg tggcagaacg ggcatttcat tgcatcgttc gggcccggca ctgggccggg
     3961 cctcccagga aggtcagcgg tacaccggca ggcgcgcggt cagctcgtgc acacgcgcgc
     4021 gcaccccggc gatgacggat tcgtcggtgg ggtggtcgag cacatcggcg atgaggttgc
     4081 cggtcagctc ggcctcggct tccttgaagc cgcgggtcgt catggccggc gtgccaagac
     4141 ggataccgct ggtgacgaag ggtttttcgg ggtcgttcgg aatggcgttc ttgttgaccg
     4201 tgatgtgcgc ctggcccagc gcggcctccg ccaccttgcc ggtgatgccc ttggggcgca
     4261 ggtcgaccag catgaggtgg ctttccgtgc gcccggacac gatgcgcagg ccccgcgtca
     4321 ccagcgtctt ggccagaacg tcggcgttct tcttgacctg ggccgcgtac tgcttgaact
     4381 cgggggtcag gacctctttg aatgccaccg ccttggccgc gatgacgtgc atcagcggcc
     4441 cgccctggat gccggggaag atcgccgagt tcacagcctt ttcgaattcg gccttcatca
     4501 tgatcacccc gccgcgcgga ccgcgcagcg atttgtgcgt ggtggacgtg acgaagtcgg
     4561 cgtgcggcac ggggttggga tagaccccac cggccaccag cccggcgtag tgggcgatgt
     4621 cgaccatgaa cagcgcgccg ttgtcgtgcg cgatgcgggc catgcgctcg aaatcgaaag
     4681 ccagcgaata tgccgaggcg ccacccacga tgagcttggg tttgtgctcg cgcgccaatt
     4741 gctcgagctt gtcgtagtcg agctcttcct ttgcgtccag gccataggag atgaagtgat
     4801 aaagcttgcc cgaggcgttc accggagagc cgtgggtcag gtggccgccc tcggccaggc
     4861 tcatgcccag aaccgtgtcg cccggcttga gcaccgccat gtacacgccc tggttcgcct
     4921 gcgacccgga gttcggctgc acgttggcgg cctcggcgcc gaagatttcc ttcaggcggt
     4981 cgatcgccaa ctgctcgacc acgtccacgt attcgcaacc cccgtagtag cgcttgcccg
     5041 gatagccttc ggcgtacttg ttggtcagtt gcgtgccctg cgcctgcatg accgccgggc
     5101 tggcgtagtt ttccgaggcg atgagctcga tgtgctgttc ctggcgcgtg ttttcttgct
     5161 gaactgccgc ccagacatcg gcgtcaactt ggtcgagagt acgtgaacga tcaaacattt
     5221 ggcttcctag attggcgaaa cactatgaat cgtggtggcg attgaaaaat ggacgttggc
     5281 tggaaatgga cacagcggat agaccggtaa ttctacagcg aatgcccaac ccattaacca
     5341 gaaaccaccc tgttcagccc cgctccggcg gctttccgcg caaaagaaat aaaaaagggc
     5401 gctttacgac aagcgccccg tgcggccgcc tctgggcgga cgcacgatcc cacccaccac
     5461 caggcttcct gcgcggggct gcccagacaa ggccgtgtca acccttggca agccgcggat
     5521 tcaaactgta gaggccggtg atggcggcct ggcccagaat gtgccccttc atggccgcgc
     5581 gcgcatcgac acccgtgaat cggccccgga caggcagttc agagatgtcc agggcataga
     5641 ccgtgaaaat gtagtggtgc acgatggaat cattccacgg cgggcacggg ccatcgtagc
     5701 cgaagtaatc gccgctcatg tcccggtccg aggcaaacca tgaggtgaaa tcattgacgc
     5761 cttgccgcgt gccgttcaac gccaacggac ccgccttgcc ccgaggggtg atcccgctgg
     5821 aatacgcgcc ttcggcgatg tgagaggtat cggccgccac atcgaccagc acccagtgat
     5881 ggaaatcgac ccggggaaga tcggatggaa tttcacgacc ttcttgattc acgtcatcgg
     5941 gctcgctggg aacatcgaca tcgtggcaga tgacgacgaa cgatttcgtg ccctgcggcg
     6001 cgtcctgcca cgcgaggtgg ggattcatgt tgtcggccag cgccacatgc gcctgcgcgt
     6061 cggccttgcc gaacgcatgg cgcgggggaa tccattgctg atcaacgaac gactcactgg
     6121 ttaatttcat gctcgactcc tgaaaacccg aaaaagccag acaccggacc ggccatcgcg
     6181 gacacggccg ccccgtttgc ggcgaacccg ccgcgccacc tcaagacaag gtgaccctcg
     6241 cgaacttgcg tttgcccacc tgtacgacgt acgtgcccgc atcgagcagc agccccttgt
     6301 cgtcgatgcg ctcgccgttg acgcgcacac caccctgctc gacgttgcgc tgcgcttcgg
     6361 cgccagacgt ggccagcccc gcctcgcgca agatcttcaa aatacccagc ggcgcccgcc
     6421 ccatccgcac ctcgggcatt tcctcgggaa tgacgccatc gcggaaacgc gcctcgaagc
     6481 gagccaaggc atcgtgcgcc gcctgttgcg agtggaaccg cgccacgatt tcctgcgcaa
     6541 gcagcacctt ggtgtcgcgc gggttcgcac ctgccttcac ctgtttctgc agcaacgaga
     6601 tctcgtccag ggaacggaat gagagcagct cgaaatatcg ccacatcagc gtgtcggaaa
     6661 tggacatgag cttgccgaac atggagtcgg gggcctcgga gatgccgatg taattgcctt
     6721 tggatttcga catcttgtcg acgccatcgg ttccttctag cagtggcatg gtcaggatgc
     6781 actggggttc ctggccatat tccttctgca actcgcgccc gaccagcagg ttgaacttct
     6841 gatcggtgcc gccgagctcc aggtcagcct gcagcgcgac cgagtcatac ccctgcatga
     6901 gggggtacag gaactcatgc accgaaatcg ggatgccgcc cttgaaacga cgggtgaaat
     6961 cttcacgctc catcatgcgc gccaccgtat agcgcgaagc cagttggatc atgccgcgcg
     7021 caccgagcgg gtcggaccat tcggagttgt agcggatttc ggtgcgctcg ggatcgagca
     7081 ccaggctggc ctgcgcgtaa tacgtctcgg cattggcgcg aatctgctcg gccgtcagtg
     7141 gtgggcgcgt cacgttgcgg ccgctggggt cgccgatcat ggaggtgaag tcgccgatga
     7201 ggaagatgac ccggtgcccg agatcctgca gctggcgcat tttattgagc accacggtgt
     7261 gccccagatg gatgtccggc gcggtggggt ccagccccag cttgatgcgc aacggcacgc
     7321 cggtggcgcg actacgggcg agctttcgaa cgaactcaga ctcgaccaac aattcgtcac
     7381 acccgcgctt ggcgacgcgc aggccggtct cgacttcggg ggtgacaggg gcttcggcag
     7441 gaatcatgga aacaggcgta tcaggaaaat atgaaaaaag gctcgaaaaa ttccaattaa
     7501 tgcgctagga tagcgtgcga acacggttct gcacagccgg cattaggtcg tcggcactgc
     7561 catcggcatt gagcggtagg tgcagggcag tcggtaaaac ggccgtgcga cgcatccccc
     7621 atcttaacaa agcaaagcac ctgagcacga cagcccgcaa ggcttgcgtc ccgggaagct
     7681 ttcgctgtat ctgaaaacac tcgaatctat cgaacaacca ggctccagct tatgtccaag
     7741 acagggaata ccagcactcc ccctgcttca aatgaccgcg acgcggtccg tcttcatccc
     7801 cccttcctca aaactggttt gcttgtcgtt gctctaggac tgttcgtcac ggccgccgcg
     7861 ctcgcagtcg tcaaccccga cagccacgac gcactgccca acctttatca ggcccgcgcg
     7921 gaacttgcgc tacccgatcc gttcccccag ccttcccagc atttctcgtc gccgttcgtg
     7981 catcagaccc gcattcgacg cggcgacacg ctggctgctt tgctgtcccg gctggacgtg
     8041 cgcgaacccg ggctgctgaa attcctgaca cacgacaagg acgcgcggtc catctacaaa
     8101 ttgtatccag gccgcctcat ggaagcgggc gtggatgaaa acggcgcgct ttcctggctg
     8161 cgctacgatc acacgccagg cgcggaagaa aacggccagt acgtcgccaa atggctcgag
     8221 gtgcgccctg acgaaaaagg cggcttcacc gcagtggaac gcaccgagga cgccgatgtg
     8281 cgcatgcgtg tcgccgaagg cgaaatccac tcgtccctgt tcggcgccac cgacgcggcc
     8341 ggcattcccg acgccatcac cctgcagatg gccaacattc tggacagcaa gatcgacttc
     8401 atcaaagatc tgcgcaaggg cgaccgcttc cagatcgttt atcagtcgta ttcacataat
     8461 ggccaggacg tcggcgccgg gcgcattctg gcgctggagt tcgtcaatga cagccacaca
     8521 taccaggcag tgtggttcca gcccaagaac ggcacgggcg gctactacga tttcagcgga
     8581 cgtagcctga aaggcgcgtt cctgcgcacc gccatcgcgt tcacccgcat cagctccaca
     8641 ttcggcatgc ggatgcaccc gctgcacaag aaatggacca aacacgaggg cgtcgactac
     8701 gcggcgccca gtggcacgcc cattcacgcc accgccgatg gcgtggtcaa gttcatcggc
     8761 caacaacgcg gctacggcaa catcattatt ctgcaacacc cccacgacta ctcgaccgtc
     8821 tatgcgcacc agagccggtt cgcgtccggc ctgcgcaagg gcgaccacgt gaagcagggc
     8881 caggtcatcg gctatgtggg cgccacgggt tgggccaccg gcccgcatct gcactatgaa
     8941 ttccgcatcg acaacaagcc cgtcaacccg ttgtccgcca gcctgcccgt ggcgttcaag
     9001 ctcgacggtg gcgaacttgc agccttccag cgcaccacct cctcctacaa aggacaaatc
     9061 gcaatgctgg cggacctgca ggatcatcat atccaagtgg ccagccgcta aacgttccag
     9121 cacatgacac acatgttgta cgcgggcctg atgtcgggca ccagcactga cggcatagac
     9181 ggcgtcctgg cggatttccc gccagtcggg ccgcctcgta tcatggccca agtatcgatg
     9241 cccatgccag aaacactccg gcgggaattt ctggcgctga acagcccggg cgacaacgaa
     9301 ctcgaacgcg cggcgctggc cgccaacgcg ttggcgaaac tgtacgccga cgccatcggc
     9361 cagttgttgg ccagggcgaa ggtcggtgcg cccgacgtcg cggccatcgg cgcgcacggg
     9421 caaaccgtca ggcaccgccc cgaagcgggc tacacgattc aactcaacgc ccccgcggtt
     9481 ctggccgaac tgaccggtat cgatgtcgtg gccgatttcc gttcccgcga tgtggcggcg
     9541 ggcgggcaag gcgccccgct ggttccggcc tttcaccagg cgctgttcca atgcgaccac
     9601 ccgcgcgccg tgctgaacct cggaggcatc gccaatgtca cgctccttgc accaaacgag
     9661 ccagtaagag gcttcgatac ggggccggcc aatgtcctga tggatatgtg gtgccaagag
     9721 aagaccggcg ccacctatga cgccgatgga aaatgggcgg ccaccggcca cgcgggcgaa
     9781 gaattgctgg acgtgatggt ggagagcgag ccctggttca gactgccgcc accgaaatca
     9841 accggccgcg accggttcaa ccgccaatgg ctggcacggc gactggccat tccagaggcc
     9901 cgtggcatgc gtgacaacga tatccaggcc accttgcgca acctgacggc acgcaccgtg
     9961 gccgacgcca ttgccgcgca cgcgccgcaa acacaagaca tcgtggtgtg cggggggggc
    10021 gcgctcaatg atggcctgat ggccacactc acgcattatt tgaaccgccc ggtggtgtca
    10081 tccgccgagt tgggcgtacc ggtgcagatg gttgaagccc tggccttcgc ctggctggcc
    10141 tgggcacatg gggcaggcca tgcggccggc ctgccaagcg tgacaggagc tcgccacgcc
    10201 acgcctctgg gcgctcgtta cccggcctga gcccgtgctc gttgcgcaag cggcccggcc
    10261 aacgccacgc cttcgggcat ccggagccgc cagcctcagg ccgagaagga cgaaccgcac
    10321 ccacaggtgg acacggcgtt gggattgcgg atgacgaact gggccccctc gagatcgtcc
    10381 ttgtagtcga tctcggcgcc gaccaggtac tggaagctca tggggtcgat gaggagcttg
    10441 acgccatcct tgtccaccac ggtgtcgtca tcgttggtgg tttcatcgaa ggtgaaaccg
    10501 tactgaaaac cggaacaccc accgccctgg acgaacaccc gcaggttcag ctccgggttg
    10561 tcctcttcgg ccagcagatc tttgactttg gcaacggccg catcggtgaa cacgagcggc
    10621 gacggaggcg cttgcaagtc gacggtttcg atcagggcat tcatgaaaaa ctccaggtcg
    10681 gcacaaggcc gcgaacaggc cacaaacggg cgatcagggc gacagtttga cggtgcgcgt
    10741 cacgcgcacc gcattgccct cgagcacgct cagcataacc gactggggcg tgaacccggt
    10801 tggcagcgcc agcttgccct cgctgcgctg gaactggtcg aagctcaggg ccagttcgtc
    10861 ggtgtttgca ggcgtcgccg gctgggcgac gcgtaaaggc atcagggtga ttttaaccgt
    10921 tttgcccttc tcctggccct gcgccacgaa gcgcaggcta cccttgaaca gggagccggg
    10981 cgcggcatta cggctgagca gcactttgta ggccaacgcc gggccctgct ggcgcacttc
    11041 cagcgcccgg atgttgaccg acccgctggg gccgggcggg aacagttgcc ggaagaacgc
    11101 caactgatcg cgggtgcgcc ccaactcgcg ctgcgcggtc gcgagcgacg cctccaaccc
    11161 cttgcgcgcg cccagttcga cgtccagctc gccctgtagt ttgtctgcct gcgcctgcag
    11221 gcggtcggca tcggcccgcg actgcgtcga caactgatcg aaccgcgctt tgagttgatc
    11281 gtatcgatca agcccccgat tcgccagcca ggcgcgcgac gcgaaaaaac cgactaccaa
    11341 cccgatcaac agcgcgagca acaaccctgg ccagcgcgac cgggctgcgt caggcggctt
    11401 cacagcggaa ccgggtatgt gggtgggctt tggagtcatg atgcatagac ctcctggccg
    11461 ccccggggtt cccggaggct ggcgcgtacg tcagggtaat accgccacgt gccgcaaacc
    11521 cgcctgctcg ggcaggccaa acatgatgtt catgttctgc acagcctgac cggccgcccc
    11581 cttgaccaga ttgtcttgca ccacgaggat gacgagatga tcggccccct cgggctgatg
    11641 cagagcgatg cgcaggtagt tggacgcccg cacagaacgg gtttcgggca ggctgccggc
    11701 gggcatgaca tcgatgaacg cctcgttggc gtagcgccgc tcgaacaacg cctggaaatc
    11761 ggtgccacgc gccccaggca gcagcggcgc ataaatggtg gagaacatgc cacggatcat
    11821 cggcaccagg tggggcacga acaacacccc aaccggcccg cccaccaagc cacccaattg
    11881 ctcggaaatc tcggcatggt gacggtgccc tttcacggca tacgccttga aattgtcgcc
    11941 ggcttcggaa tacaggttgc ccacggcggc tttgcggcca gccccggacg tgcccgactt
    12001 gcaatcggcg acgatatggg cgggatcgat cagatggcgc ccctgttcca gcagcggcag
    12061 caggccaagg atgaccgtgg tgggatagca ccccggatta cccacgaccc gcgccttggc
    12121 gatcttgtcg cggttcacct cggccagacc gtacaccgca tcggccagga tatcggggca
    12181 cgcgtgcggc atcttgtacc acttctcgaa ggcccgggta tcgtgcaggc ggaagtccgc
    12241 cgccagatcg atgatacgga cgtcgtggcg caacaattcg cgcgcctgat ccatggcgac
    12301 accgtgcggg gtggcgaaga acaccacatc gcaatcagag agcgacgccg cgtcgggagt
    12361 ctggtagctc aggtcgacca acccgcgcaa gttgggaaac atctcggcca ccggcatgcc
    12421 atcctcggat cgtgatgtga tggccttgag ttcgacgtcg ggatggaccg acagcaaacg
    12481 cagaagctcg acgccggtgt acccggtgcc gcccacgatg ccgaccttga tgcgcgatga
    12541 ggatgcctgg ctcatgttga ttcccaatac aggttgctcg tcaaaacatt attatgcgac
    12601 atcccggccc ctggtttggc gggagtgacc caaatgtcac atgcctgaag ccacgcgccg
    12661 gcctgccaca agccaggtgc gccacaaaag ccaaaagccc cagccgtggc cggggctttt
    12721 ggcggccgtg ccggacgcgc caggcgtccg cacggaacga tcagcgcttg ctgaactgct
    12781 tgcgccgacg cgccttgtgg aagccgacct tcttgcgttc gacttcgcgg gcgtcacgcg
    12841 tgaccaaccc ggcttgcttg agcggggatt tcagcgtttc gtcgtagtcg atgagcgcac
    12901 gcgtgatgcc gtggcgcacc gcgccagcct ggccgctttc accaccgcca tggacgttga
    12961 tgtgaaaatc aaacgactcc aggtggcccg tgaggctcag gggctgccgc acgaccatgc
    13021 ggccggtttc gcgagcgaag tattcgtcga cgggcttgcc gttgacgacg atcttgcccg
    13081 accctttctt gaggaacacg cgcgccaccg aagtcttgcg gcggccagtt ccgtaattcc
    13141 agttaccgat catgaccttt ccttagaatt ccagcggttt gggctgctga gcagaatgag
    13201 ggtgctcggc gccggcgtaa accttcagct tcttggccat ggcgtagccc agcggaccct
    13261 tgggcaacat gcccttcacg gccttctgca gagcgcggcc aggaaaacgc tgctgcattt
    13321 tctggaagtt ggtttcgtac acaccgccgg gatagcccga atgccggtag tatttcttgt
    13381 caagcgactt gttgccggtg acgacgatat ccgccgcgtt gatgatgaca atatagtcac
    13441 ccgtatcaac gtgaggcgtg aattctggtt tgtgtttacc gcgcaaacgt cgtgcgactt
    13501 cgctggccac acgacccagg actttgccct tggcgtcaat cacgtaccag tcacgcttga
    13561 cttcatgcgg cttggccacg aaggtcttca tgatggttcc tgaaaaaata ttgaattgcg
    13621 cacctggcat ccaggcgcac cgctttgttt cccggcgaat tcacaatgac cctgtctagg
    13681 cgttgtatcc cgcccgtaca ctgtcgctgt atttgctttg ctgtatcgct tatttgcaga
    13741 tacctctaca cagatacgcc acattgattc caccgcaccc gccgtgccaa cctggccaca
    13801 gccaacaacc gaagcgatga atcaaggccc agaaaatccg ctggaaagct tgctatcata
    13861 gcacaatcca ctgatttccc aacaaatagc tggaaccacc ctgccagggc gaactatgtc
    13921 gcgatcgcca gtaagccgcc acggcatcac caggcggcca cggcgatgcc gtggcgggtc
    13981 gctggctgag tgaacgcacc ctcgttgacg cgacgatcag ggtccgaaca ggtaagtacc
    14041 cggccagtta tcgttttcca tgggttttaa tctgcaacga attgcgtgat aatccgatcc
    14101 ttgccaaacc caacagggcg caggcggcgc aggaatgccg gggatacacc cattcccgcg
    14161 tgtttccaac tgaataccaa gaaagggcgt tcaccatgca cagcacacgc aagatactcc
    14221 tggcttccct catcgccgcg ggcagcctgt ccgtcgcgca cgcgggcgac atcaacatcg
    14281 gcatggctga agccctgtcg ggcggcgccg cccagtacgg ccactccatc aagaatggct
    14341 tcgagctggc tgcctccgag atcaatgccg cgggcggcat caacggcgac aagatcaaat
    14401 tcgtcgtcga agacgaacaa ggcaagaaag acgaggccat caacgtcttc aagaaactga
    14461 tcttccagga caaggtcctg atgatcttcg gcccgaccct gtcgaattcc gcccaagccg
    14521 ccgacccaat cgcccaggca tccaagacgg tcgcgttcgg cacgtccaac accgccaatg
    14581 gcatcacgtc catcggcaac tacatcttcc gcaattccgt gaccgaggcc gacgtcctgc
    14641 ccgtcacgct tgccgtcgcc cagcgcaaac tcggcatcaa gaaagtggcc gtgctctacg
    14701 gcaatgacga cgtcttcacc acgagcggct acgacaactt caagaaggcc ctggccgacc
    14761 tgaagattcc cgtcacggcg acggaaacct tctccaaggg cgatgtcgac ttcaaagccc
    14821 aactgaccaa aatcgaaagc acgcacccag acgccatcgt cctgtcggca ctgctggccg
    14881 aaggcgcccc catcatggtg caggcccgcc agctcggcat caaggttccg ttcattggcg
    14941 gcaacggcat gaattccgtc aagatcttca cgctggcgcc gggcgcggcc tctaacaatc
    15001 tgtgggtggg cagcccctgg tccatcgaca accacacgcc cgagaacgac aagttcgtcg
    15061 ctgcgtacaa ggccaagttc cactcggctc ccgatcagtt cgcggcacag tcctacgatg
    15121 ccacacacat cgtcgcgcag gcgctcaagc acatcaagct caccggcaac ctcgccgccg
    15181 atcgcacggc attgcgcaac gccctgccct cggtcaagtg gaccggcgcc accgggcctt
    15241 tcgagttcgt ccaggccaag aacaaggccg gcaaacccgc tggttatgac gccaagcaaa
    15301 ccgccatcat cagcgtgacg gacaacggca aatacaccat cgaaaagtaa gcgcacccca
    15361 agaacgtcgt agccacggaa tagcaccgca tgctggaaca gcaactcatc aacgccctgt
    15421 ccctgggatg cgtatacgct ttgttcgcgc tgggcttcac gctggtgttc ggcgttcttg
    15481 gcgtcatcaa cctgtcgcat ggcgcggtct tcatggccgg cgcctatgcg gcgctgtatg
    15541 ccatcgccac gctcggcctg ccgctttggg cggggctgat cgtgtcattc ctggctgcgg
    15601 gcgcgctggg cgcgatcata gactggctcg ttctgcggcc gctgcgacac cgtggcgcgc
    15661 cgcacctcat cccgatgatc gccaccatcg gcataggcat catcctcacc aacggggcgc
    15721 aaggcatctt cggccccaac aatctgcgct tcccgacggg cacgattccc aaccagtcgt
    15781 tcatgctggg cgggctgcac atgaccgtca ttgaagtcgg catcatcttt ctttcgttcg
    15841 ccctcatgct ggcgctgatg tacgtcatgc gccgcacccg cttcgggcgg gcgcttcgcg
    15901 ccatcgccga atctcccaag gcggcctggc tactcggcat caatgtcgag cgcctgttcc
    15961 tgaccacatc cttcgtggcc ggtggcctgg gcggcatcgc gggcgtgctc atcggcctgt
    16021 attccaacgc tctttatccc ctgatgggcg aacccattct ccgcaaaggc atcgccgtgg
    16081 tcatcctggg ggggctgggc agcatccccg gtgccatgat cggcgggctc ttcctcggct
    16141 tctccgaagt cctctcggtc gctttcatcg gctcaaccac acgcgacgct gtggcgttcg
    16201 gcctgctgtt cctggtgctg ctgctgcgcc cgcaggggct gttcggcaaa gctgtccagc
    16261 gcaaggcata aggcaacatg ggtagtctgg caaccttctg ggccatttat ggcaatgtcg
    16321 tcatgtcgct gggcaccaac gccctgctgg ccctgtcaat ctggctgacg ctggtctgcg
    16381 gcatgctgtc catcgccaat gccgccttca tgggcatagg cgcctacgcg gccgcgatct
    16441 gcaccatgag cctcggcatg ccgtttcccg tctcgctcgc ggcgggcatg gtggccccgg
    16501 ccattctggc ggccatcatc gggctaccca ccatacgcct gtcgggcgtg tacctggcca
    16561 tggccaccct ggctttcggc gaggtggtcc gggtgatcgt gctcaacacc caatccatca
    16621 ccggcggcgc gctggggttg aacggcattc cgcaactcac gcaatggtgg cacgtgctgc
    16681 tcgccgtcat cattgtgctg ctggttctgt ggcgcctgaa ggtctcgaaa gtgggccgtg
    16741 ccttcgacgc catacgcggc gacgaaaccg ccgccagcct gatgggcgtg aatgtccgct
    16801 ccaacaagct gctcgccttc gtcctgggcg cggcgatcgc cggcctggcc ggcgcgctcg
    16861 acgcccacct gaccttttac atcggcccca acgaatacgg cttcgaacgc gccgtggaaa
    16921 tcctgaccat gaccatcctg ggtggcatcg gcgggttggt cggcccggtg atcggcagca
    16981 gcatcatcac cttgctgccc gaagtcctgc gtggcttcaa cagtttccgt ctggtcgcca
    17041 acggactgat cctcgtgctc atcgtgttgt tcctgccccg cggtatctgg gaccccgccc
    17101 gctatgcgca ttggcttcgc aaggggagca aacgccgtgc ttgagctctc ctcgatctcc
    17161 atccacttcg gtgggctgca tgtcctgcat gacgtcaatg tcaaggtgcc tgaaggtgcg
    17221 atcttcggcc tgatcggccc caacggcgct ggcaagacca cggtgttcaa cctgatcacc
    17281 ggcctgctct ccccgaatgg gggcgacatc ctgttcgagg gccgcagcct gatcggccag
    17341 aagccgcatc gcatcacgcg cctgggcctt gcccgcacgt tccagaacat ccgcctgttc
    17401 aaagaaatga cgctgctcca gaacgtggtg gtcggctctt accggcacat gcattacggc
    17461 gtgtccgatc tgttgttcgg gctacccgcg ttcaggcgcc aggagaccca ggcgcgcgac
    17521 cgcgccatgg agctcctgac ctggctcaag ctggatcaca aggcccatga cctggcggac
    17581 aacctttcct acggcgagca gcgccgcctc gaactggcac gcgcactggc caccgagccc
    17641 aagctgttgc tgctcgacga acccgtggcc gggatgaaca ccggcgaacg cgccgaactc
    17701 atgcacgaaa tccgggccat tcgcgaccgc ggctacacca tcctcatgat cgagcacgac
    17761 atgcatttcg tcatggggtt gtgcgacgag atcgcggtgc tgaatttcgg caaaatcatc
    17821 gcctcaggtc aacccgaggc gatccgcaac aacgaagaag tcatcgaggc ctacctgggc
    17881 cgcgacgacg acgacaccga ggtgcatgca tgagcgccgc gctggaagtg cgcaacctga
    17941 ccgtccacta cggcaagatc gaggcggtgc gcggcattga cctggcactc gagcagggcg
    18001 gcatcacggc gctggttggc gccaatggcg ccggcaagtc gaccacactg ctcgccctgt
    18061 ccggcctgtt gcccaaattg gcggggtccg tgctcttcga gggtgaagac atcacgcggc
    18121 tcgcaccgca ccaggtcgtg gcgcgcggca ttgtgcaggt acccgaaggc cgggccatcc
    18181 tgaccaccat gaccgtgcgc gagaaccttg aactcggggc gtaccgccag ggcggcggcg
    18241 tggcgctcaa ggatttcgac tacgtgttcg cgcttttccc gaggctgaaa gaacgcctcg
    18301 aggggttggc cggcaacctg tcgggcggcg agcagcaaat gctggccatc gcacgcgcgc
    18361 tcatggccaa gccacgcctg ctgctgctcg atgagccgtc catggggctg gcccccatcg
    18421 tggtccagga aatcttccag gccctgcgcg ccatcaacgc cgacggcttg actctgttcc
    18481 tggtggagca gaacgtccgg caggcactga agatctccca gcaaggctac gtgctggaaa
    18541 acggcagcat ggcgctgacg ggaaccggca aggaactgct cggccatccg cgcgtgcttc
    18601 aggcctacct gggcgtatag cgccagagaa gctccggcga cagccctttc gcgccggctc
    18661 ccgccgcacg ggcactgaaa cgccacggcg ccggcgcatg gcccggtttc aggtacccgc
    18721 caccgccatc tgctcgatca gaatcgagcc tgtggtcttg gacccccggg tgatggtgtc
    18781 ggcccccacg gcaacaatcc gttggaacat gtcggccaag tgcccggcga tggtgatctc
    18841 ttgcacggcg tgcatgatga caccgttctc gacccagtag ccaaaagcgc cacgggaata
    18901 atcccccgtg acgtaattca cgccttgccc catcagttct gtgaccagca gcccggtgcc
    18961 cagtttgcgc aacatggcgc ggaaatcgtc tttcggacgc gtcaggcgcg atgacaggac
    19021 aagattatgc gagccgccgg cattgccggt ggatgccatg ccgagtttgc gcgctgtgta
    19081 gctggacagg aaatacccct gcagcacacc cgcgtcgacc aatcggcgag gctgcgtgcg
    19141 caccccttcg tcatcgaaag gcgcgctgcc catggctccc acgatgtgcg gatcttcgtc
    19201 gatatcgaca tgcccggaca tgaccggttt acccagtgaa tccagcagga aactggcctt
    19261 gcggtacaac gcgccgccac tgaccgcctg caccagcgcg cccagcaacc ccaccgccag
    19321 cggcgcctcg aagagcacag gaaacttgcc cgtgggaatg cgccgcgccg acaacctggc
    19381 cagcgtgcgc tcggcggcat agcggcccac ggcctctggg tcggccagat tggccggatc
    19441 gcgcgcggcg ctgtaccagt agtcacgctg catggccgcg ccttttccgg cgatcggcgc
    19501 caccgacagg ctatgacggg aatacggata tccgccgagg aatcctcggc tgttgcccat
    19561 gacgaaatgc ccctcgagcg tgccaaccga tgcgccttcg gtgttggtca cgagcggact
    19621 gaccgcgcga gacgcgcgtt ccaccctcag cgccagttcg gcggcttctt cggccgacaa
    19681 gctccaggga tgatgcaggc gcagatcgga cacgtcatgc gcgagaaatt ccgcttcggg
    19741 caaccccgca gccggatctg gcgccgtgta gcgagcgatg tgccacgcgg cctcgaccgt
    19801 ctggcgcagg gcgtcggcgg aaaaatcgga cgtcgaagcc gacccgcgcc gctgcccggc
    19861 gtacacggtg acatccagcg agcggtcgcg cgtctgctcc accgtttcga gcgcgagctt
    19921 gcgcaccgag accgacaggc cacggctttc cgacacctcg gccaccgcgt ccgaggcgcc
    19981 cagcgcgcgc gcctcgtcaa gcgcctggcg cacaagatcc cggaaccggc cttgattctc
    20041 ggcgatcggc aattctgaaa cggcgttctg attcatatga gactctttca ttcgatccgg
    20101 aaatgggctg tcgggaatgc cacccgaacg ggccggccca acaaaggatc ctctgggcaa
    20161 gccaccgcgc cgcacgacca cctgtggctt atccagagga tcctcaagac ggttatcata
    20221 gcgaattaat gccgccctct tccaacccca tgccccacaa cgataccgca ccgctggatg
    20281 cgccgtcgga accgacggac gacgacaaac ccagcaaatc ccaggtcaag cgcgacatgc
    20341 acgcgctgct cgaccttggc aaacaactgg tggatctgcc cgttgaccga ctccggcaac
    20401 tcgatctggc cgaacctctt tatgacgcca tcctgctggc ccagcggaca cacagccgcg
    20461 aaggccgacg gcgccagatc cactacgtcg gcaaactgat gcgcaaagcc cccgcggaag
    20521 ccatccgcgt gcagctcgac acctgggcca acggttcacg cgaggaaacc cggtccatgc
    20581 accgcctgga agcactgcgc gatctactgc tcaacgacga cgatgccctc acccagctgc
    20641 tgcaggacta cccgggcgcc gacgtgcagc aactacgcgc cctgatccgc gccggccgcc
    20701 gtgagcgaca gcagaaccag accctgcaag ccggccagga acccatccgc aaacactatc
    20761 gcgccctgtt ccaggccctc aaggccctgt cggccggcac ccccgccgac acatccgcca
    20821 cagcgcagcc cgacacgccc tcgactgaag cgcctgcctc ctgaatgaac acgaacgtgc
    20881 ccgcgatgat ccacttcccc atatgaacac gaacacgctc tccgaccgca tcgaaataga
    20941 aaccgccccc aaccccacgc acgccatcat ctggctgcat ggcctgggag ccgacggcaa
    21001 cgacttcgcc cccatcgttc ccgaactgcg actgtcctcg aatccgcgtc tgcgcttcgt
    21061 ctttccccat gccccggtcc gccccgtcag catcaacggc ggcatgccca tgcgcgcctg
    21121 gtacgacata cttacgcccg acctcgtgcg tcgtgaagac gaccagggca tacgtgattc
    21181 cgaagccttg gtgcgcacgc tgatcgaacg cgaaaacgca cgcggcatct ccaccgaaaa
    21241 cctggttctc gccggcttct cccaaggctg cgccatggcc ttgcacaccg gcctgcgact
    21301 ggaccacccc ttggccggca tcatgggcct ctcgggctac ctgcctctgg ccgacacggt
    21361 aacggccgag cgccacaccg ccaaccagaa cacccccatt ttccttgccc acggcaccct
    21421 ggatcccgtc gtcgtgctgc cgcgcgccca ggcatcgcac caacgcctga ccgacatggg
    21481 ctacgacgtc cactggaaga cctaccccat gccgcactcg gtctgtccgc aggaaatcgc
    21541 cgacatcgcc gatttcctga ataccgtatt gaagtaacca acccaccacc ccacgacacc
    21601 accaccccac gacacagcaa gagcggggcg ggcacccagg gtcgggccga ctttgtcgca
    21661 agccggccaa ccgactcaag aagcaacggc gccctccccc aaatccaacc acacccgccg
    21721 catccccgga tcctcctcgt cgggatcatt cttgaacccc agatgcgtca tcaaccccag
    21781 catcggccga ttgcccgtca gcaccacccc gtctatatac gccagcccct gctcccgcgc
    21841 cgccgtgatc aaaccccgca tcagcacatg ccccaatcca tggtgctgcc acgcatcggc
    21901 gatcaccagt gcatactccg ccccccgccc gtccgcattg cgcagataat gcgcgaaccc
    21961 cacgatctcg tcgcgcggat gcccccggtg ctcagggttc ggcacctgga cagccgccac
    22021 caacgccaat tccctgtcgt aatcgatgcg cgtgtaccgc gccagcatcg ccggcgtcaa
    22081 ttcgcgcagc atcgacacaa accgcatgta gcgcgattca tccgacaggc cccgcacgaa
    22141 aacctgcagc gcctcggcgt cctccggacg gatggggcgc atcgtccacg gcgcgccatc
    22201 cttgaacacc cgggcctgca ccagacgggt gggatacgga tggatcgcca tatgccgata
    22261 ccccgtcgtc tgcggcgcgg cactctcgat ggccgatgtc aggctcagtg tgacgcggga
    22321 ggtcagcaac cgcgcgccat gcgcgaacaa catgtcgatc tcgagatctt ccatgtccgg
    22381 caggtcgcag accatgtccg aaatcctttc caaagcctcc tgtaaagatt cgcaagccgc
    22441 gactcccagt tcgcgcgaca ggacacggcg ccacagcgca ctgcgccgaa ccaactgccg
    22501 agccagatgg ccgttgagcg gcggcaactc caccccctcg tcgcccgcgg acagcacggc
    22561 ctggctcccc cccacgccaa acaccacgaa aggcccgaaa cggggatcgc gtcgaacccg
    22621 tatggccata ggcaccgtgt tctcgccatg gccatcatcc tcgcccggac ccgacctgag
    22681 tgtgatcggg acatggaaac acgcaaagag gtcctgacat tcggaagggg taaggtcccg
    22741 ccggccatcg gcgcgaacgc cttccagcac ggcccgcgcc cgatccgacc gcaccggcag
    22801 tccgagcggc tccgggggaa gtatctggcg cgcgagctcc tggttatagt gataggacgc
    22861 aagaatgcca aacgcattgg ccgccgtctc cggcgtgcga aaggccgggg cccccacttc
    22921 gtcaagaaca tggcgcaacg ggcgcatgcc cgcgtcaccc agcagagaaa caacaaccgg
    22981 cttgcgcgct tcgcgggaaa gccgcgccag caccgacacc accccggcga tatccgccaa
    23041 cggatccggc gtcagcagca ccagcacccc gtcgacaccc ggatcccgca ccagtacccg
    23101 caacgcccgg tccaccgtgc caggatccaa tggcgcgtgc gtcaccaccg ggttctccac
    23161 ctgggcgcgc gcatccagca tttcgccaag ctcggtggcg gtggctctgg acagctcggc
    23221 gcaaaacacc ttggcgccgg gccccatcgc atcgatcgcc agttgcgccg cgccatggcc
    23281 gttggaaaac aacgcgacgc gccggccacg cgggcgttgt gcgtaggtca gcaccttgag
    23341 cgccgaaaac agctggacga aataccggac ccgcaccgcg ccaacacgcc gcaacaacgc
    23401 attgaacact gcgtcctcgg acgaggctgg atcaaaccgc ccatgcccgg tcttgagcac
    23461 caccaccggc ttgatgctgg ccgccgcgcg cagggcactg ccgaatcgac gcgatgatgc
    23521 cgtgttctcc aggtacagca cgatactgtc ggtttgggca tccatggcca gataatcgag
    23581 cacggcggcg acatcgatga ccgcctcgtc gcccagggag atcgccgtgg aaaagcccag
    23641 acgaatatcc tcggcccaat ccagcaccgc gccggtcagc gactgcgact gcgtcaccaa
    23701 cgccaccctg cccaccagcg ccggccgggt ctcgtggctg agattcaatc cgtagacagg
    23761 gcgttgcacg cccgtggaat gcggccccag caactcacaa tcatgcaggc gggcccaggt
    23821 ctggcaatag gccatgtcct cgatcgggtc agtggtcgtc tcagggtgcc tcagcaacac
    23881 caccgcgcgc gggcgaagcg gtgccaaggc ctcgagcgcc cggggaatat ggacggatgc
    23941 cacacacacc agcgcggcat ccagcctgtc accctcggcg acacccgccg ggcgcggcgg
    24001 taccaccatg gcgccatcgc ctcccacctc cacgaactcg acgcgacccg ccagcgccga
    24061 cggcgccgca tgcgcgatgg gcaggtctcg atcggccagc accagcagcg aacacggctc
    24121 gaacaacgcg gcaagacgat gacgcaacat actctgatca acccctgttc ccatgcggaa
    24181 ccttttcgta ctctaaaata acggcttcat tcgaagcctt gccgatcaag ccatgaccca
    24241 agccgttccc gccgcgacgc ccgcggtacg cacccgtttc gcaccgtccc ccactggttt
    24301 cctgcacctg ggaggcgctc gcaccgcgct gttctcgtgg gcctacgccc gccaccatca
    24361 tggaacattc gtgctgcggg tcgaagacac ggatctggag cgatccaccc ccgaggccgt
    24421 gcaggccatc ctggacgcca tggcatggct cgacatgcgc cccgacgagg gcccgttcta
    24481 ccagatgcag cgcatggacc gctaccgcga agtggtcgcg acaatgctca aggccggcac
    24541 cgcctaccat tgctacagca gcccggaaga agtcgaggcc atgcgtgaaa aagcgcgcgc
    24601 agccgggttg aaaccccgct acgatggcac gtggcgcccc gagcccggca agaccctgcc
    24661 gcccgttcca gcgggccgaa agcctgtcgt gcgcttcaag agcccccaaa ccggcaccac
    24721 cggctgggat gacctgatca aaggccccat cagtttcgac aacaccgaac tcgacgacct
    24781 ggtcatcgcc cgccccgacg gcacccccac ctacaacttc tgtgtggtgg tcgatgactg
    24841 ggacatgaaa atcacccacg tcatccgtgg cgacgaccac gtcaacaaca cgccccgcca
    24901 gatcaccatt ttacgcgcgc tgggggccac gccgccgcaa tacgggcatg tccccatgat
    24961 cctcggcccc gacggcgaga aactgtccaa gcgccacggc gcggtcaacg tcatgcagta
    25021 cgaccgtgac ggctatctgc cagaagccat gatcaactac ctcgcccgac tggggtggag
    25081 ccacggcgac gacgagattt tcagccggga acaactggtg gaatggttcg atacccggca
    25141 cctgagcaag tcggccgcgc agtgggatcc caagaaactc aactgggtga atgcccatta
    25201 catcaaggcc ctgggcgacg ccgacctggc cgcgcgggtc gcgccgcgca tccgcgaact
    25261 ggggggcgat ccggacgccg tcgacctgca agcggtggtc gcgctgctga aggatcgcgc
    25321 cgaaaccctc aaccagctgg ccgacggcgc catgctgttc tgcggtccct accagcctgc
    25381 tccagaagaa ttgcgcggcc agcatctgga tgccgatgcc gttggcctgc tgcgggaatt
    25441 cgcgcgccgc gcggccggcc tgcccgactg gtcggccgct ccgctgtcgg cgctgatcaa
    25501 ggccatgctg gaagaacacg gcgtcaagat gcccaagctg gccatcccgt tgcgcgtggc
    25561 ggtcaccggc cgcaaacaga caccagccat cgatgccgta ttgaccatcc tggggcgtgc
    25621 cacggtgctg gcgcgcctgg acgcgctcag cgaccaataa ggcaggccat acccgggccg
    25681 atcagtaagg cagtcgatac cccggcgaca tgcgtgtggc tttgaccagg ccattctcgc
    25741 cacgcagcgt caatgtcctg gccgactgcg acaccaccgt gtagcggttg gtgtagatgg
    25801 ggtcgccggg ttccactggc accccgttcg acttggtggt gcgcaacacc agcacgcctc
    25861 catcgcggga ccagcaaccg ctttcctcca ggccttgggg cggggtgcca gcgcgcttga
    25921 tctggtcttc gtatcgcatg gtgccatcgg ccgacagccc gaacagcacg cggtacttcc
    25981 ccgccacacc ccgacgatgg aattcgctca gccacgtgcc aacggcggga tcgcctggct
    26041 gcgccgcccg gcacaccact tgcgcgggcg cggccggttt gggcccaggg cgcggcttgg
    26101 gtgaggaaca tccggccagc acaataccca ggccggcggc gacggcgaag aggccgacgt
    26161 gaaacatggc ggtgcgcata ccaatacctt gcaagcatcg ggcgtgggca cccaccgcgc
    26221 attcaagacc acaatcgaca cggtggtatg gcgcgcgttg gcaagtgccg gccctacaat
    26281 gaatccgccc ggcgaacagt acgccggacc gcgccgcgac gtcatataaa cagcggccga
    26341 cggcgcgaat gagtgtaccc gaatgccaca ggcgccggct gtgacaattc ataaaccgcc
    26401 ggagccgccg cgccatgagt cattccgttc tcatcacctt gcacctgttc gccgccatca
    26461 tcttcgtggg catggtgttt ttcgaagttc tgatcctgga aggcatacgc gggcaggtgc
    26521 cgcgcgacgc catgcgctcg gtcgagatcg ccatcggcaa gcgcgcccgg cgtttcatgc
    26581 cgttcgtgat cctagtgctg tacggagccg gcatcggcat ggcctggcag ttccgcggcg
    26641 cgctggcgca cccgttcgcc agcagtttcg gcacgctgct gagcctgaag atcctgctgg
    26701 ccctcagtgt gctggtgcat ttcatcaccg ccatgacgct tagcgccaag ggcaagctca
    26761 agacccggca cttccagcgc atacacgtca gtgtcttcct gcacatgata ggcatcgtct
    26821 ttcttgccaa ggcgatgttc tatctgcact ggtagccgcg tcactcgcgg gagctcctgc
    26881 ggcgcggcca gacaaccaca tccagaaaaa cgacgtgccg aagtgaatcg cgcgcggtca
    26941 aagcgccagc ggcagctcca cttcgtgctt gatctcgcgc agggcgacga tggtccgggt
    27001 ctggcgcacg cccggaagat tgaacaggaa gcgatgcagg aaatcgtcgt aggcttcggc
    27061 gctttccacc agcaccttca gcagaaagtc cgcatcgccg gtcacggtat agcattccag
    27121 gatctcgggc gcgtcgcgca ctgcgcgaat gaaattgtcg acgatctcgg ccgaatgccg
    27181 ttccaggctg atctgcgtga acatgcagcc gcgcaacccc acatggctgc ggctgacccg
    27241 ggcgttgtac ccgagtatcg cgccgctgga ttccaacgat ttgacccgcc gccacaccgg
    27301 cgccgccgac aacccgacgg cgtcggccac ttgctgcgcc gacgcgcgcc cgttcttttg
    27361 caggtactga agaattttca gatctgtttt gtccatatat cacaccataa cgaaacaatc
    27421 atttcgtgat atagggattt tacgacacaa aatagcaata aaacccaagc cgtgactctc
    27481 tatcatgaag gagacaaatc ccccatttcc gatgatggag tgtgttcatg cccggcacca
    27541 ccctggatac ccggtaccag ctcactgaca acctgacggc gacctccggg cgcatctatc
    27601 tcaccggccc gcaggccctg gttcgcatgg cgctgtctca acggcgcatc gatgccgcgc
    27661 gcggcctgaa caccgccgga ttcgtcagcg gctaccgggg ctcgcctctg gccggcgtcg
    27721 acatggccat gtggcgcgcg caggctttgc tggaggaagc gcatgtccga ttcctgcccg
    27781 ccgtcaacga agatatggcg gccacgatga tgatgggcac ccagcagacc ggggagcggg
    27841 ccgaccgcac ggtcgagggg gtgttcggca tgtggtatgg aaaagggccg ggggtggacc
    27901 gggccggcga tgccatccac cacggcaacg ccgccggcgc ctcgcgtcac ggcggcgtgc
    27961 tgatgatcgt gggcgatgac cacaccgccg cgtcatcctc gatcgggcat gccagcgaaa
    28021 cctccctgac cgcctggagc atccccatca tcaacccatc ctcgatcgag gaatacgagt
    28081 atttcggcct atggggctgg gccctgtcgc gcttctgtgg cgcctgggtg gcgttcaagg
    28141 ccatcacgga aaccgtggaa agcggccgat cattcgaatt gcagtccata ccggactttt
    28201 ccgcctgcct gaacgatccc cgtgcgggga aactgcaata cagtgcgcgc gaatttctca
    28261 cgccggccat ggaacaccgc atcgccgaac gcctgacggc ggtccaggca ttctcgcaac
    28321 tgcaccgcct ggaccgcctg gtcaatccgg cgccacaggg agcgctgggc ctggtgacca
    28381 ccggcaaggc cacgctggac accgaggagg ccctgcggcg tctggcacga tccggcgccc
    28441 acctgccgcc gatccgccac tacaaggtcg gcctgacgtg gccgctggac cgcgccggtc
    28501 tggacgcctt tgtcgacggc ctcgagcaca tcctggtcgt ggaagaaaaa gcgcccatcg
    28561 tcgagaacca gatcaaggaa ttgctgttca accgtgcgcg ccgccccacc gtgcagggca
    28621 agaccgatct gcgcggccag ccactggtca caggcgaagg ccaattgaat ccaacgctcg
    28681 tggccaccgc cctgcgcgga tggctgcgtg acgccggcgc gctgacgctc cccgaaccaa
    28741 cgggccgcgc accggtgctg gaccccgcca cgacccacgg cctggcccgc cgcccgtatt
    28801 tctgctcagg gtgcccgcac agcacctcga cgaaagtccc ggaaggcagt caggcgctgg
    28861 ccggcgtcgg ctgccattac atggcgacct ggatggaccg cgataccagc ggcctgacgc
    28921 aaatgggcgg tgaaggcgta gactggatcg gactggcccc gttcacgcgc acaccgcacg
    28981 tcttccagaa catgggggaa ggcacgtatt tccattcagg gtacctggcc atccgccagg
    29041 cggtcgccgc gcaggcccac atcacctaca agatcctgtt caacgacgcc gtcgccatga
    29101 caggcggaca acccgtggat ggtcacttgt ccgtgccgga catctgccgg cagatgctgg
    29161 gcgaaggggt ggcgcgcgtg gtcatcacga ccgacgagcc gcaaaagtac cagggcgtgc
    29221 cactcgtcca gggcgtcgcc gtgcatgacc ggcacgaact ggacagcgtg cagcgcgaac
    29281 tgcgcgacat ccccggtgtc accgtgctca tccacgacca ggcctgcgcc gccgagaaac
    29341 ggcgacggcg caagaagaaa gcgtttcccg acccaccccg gcgtttattc atcaacacgg
    29401 cggtctgtga gggctgtggt gactgcggca cccagtcgaa ctgcctgtcg ctggtgccgg
    29461 tggaaacgcc gttcgggcgc aagcgcgcca tcgatcaatc cagctgcaac aaggactact
    29521 cctgcgtgga cggcttctgc cccagcttcg tctcggtgat gggcggcacg ccacggcgca
    29581 gcgcacgcat cgcggagccg gggaatcagg cgcggctcga aaccctgcgc cggcaactgc
    29641 cagatccggt catactggcg ggcggcgagg tctgcaacat cctggtggcg ggtattggag
    29701 gctcgggtgt cgtcaccttg ggtgcgctcc tggccatggc cgcgcacctt gaggggcggg
    29761 cggtatccac gttggacgtc accggtctgg cccagaaagg gggtgcggtc atcagccatg
    29821 tccgcctcgc gcgtggggcg caagcccgtg gcgtcgtgcg catagaccag cgccaggccg
    29881 acgtggccat cctgtgcgac ccggtcgccg cggcccggcc cgatatcgtc gccaccctga
    29941 aaccctcggc cacgcttgtc acattgagca cctacctggc ccccaccgcc gagttcacgc
    30001 gcgatcccga cacgaacctg gatgcctcgc ggctggtcgc ggggctgcgc gatatcgcgg
    30061 gcgccgccaa cacccgcgcc ctggatgccc atgaactggc cttgtcgctg atgggcgacg
    30121 cccagctttc caatgtgatg atgctgggct tcacctggca gcgcggtgcc ataccgctgg
    30181 accgggcctc cgtcgaacgc gccatcacac tcaacggggt ggccgtgccc gccaacctgc
    30241 aggcgttcga actgggccgg atcgccgcct ggcaacccga caccctggac aagatgctgc
    30301 acccatcgcg ccagagccgc gtcattccgt tccccgaatc gctggacacc gtggtggaac
    30361 gctgccgcga cgatctgacc ggctaccaaa gcgccgccta tgcgaagacc tacaccgaca
    30421 ccgtcgcggc ggtcgcgcgc gccgaacagg cgctgcaccc ccaggccaaa ccggtgctgg
    30481 cgctggcggt ggcccgctcg ctgcacaagc tcatggccta caaggacgag tacgaagtcg
    30541 cacgcctgtt ctctgacggc cgcttccgtc gcgaactgca atcccgattc gaaggcgact
    30601 tcactctgcg cttccatctg gcaccgccgc tgctcgcccg tcgcgacccc cgcacgggcg
    30661 cgccacgcaa gatgacgttc ggcccggcca tggaggcggt tttccgcgtg ctggcgcatg
    30721 gcaggcgtct gcgcggcacc tggctcgatg tgtttggcta cagcgcggaa cgcaggacag
    30781 agcgccggct cgcccgccag tatcgggcgc gcatcctcga catgctgggt aaactgaacg
    30841 aacgcacctt cccgatcgcc gtcgagctcg ccgcgctgcc cgacatggtc cgcggattcg
    30901 gccacgtcaa gcaggccagc atcgaccgct acgaccagcg tctggcggag atgacccgaa
    30961 ccctgagcgc cgaacccggc cacccggcgc ataccccgag cgtcgtgcgc cgcgccggat
    31021 gaagtgattt tttacaacat ggaacgcccg gccgccctgc ccgagggcgg tcgggcgttc
    31081 ttcctatgga caccccgggg atatccacaa aatatccaca gactatccac tggaataccc
    31141 acagaacacg cttgcctggt ctgaaaacga gcactacaat tagtacataa caagacgttg
    31201 accactatat attgtggttt tattcaaata atcaaggttc tagcgcacgg ccggaaaccg
    31261 gctgcccgcc gtcggccttc cacccgaaaa aaatagtcgt ccggcgcata cgtcgatgcg
    31321 cctttctctg tcaggagccc acatgcagac cactcagaac gccgaatccg ggcgtgccgc
    31381 cacgccggcc cccgccctcg atccggtcaa ggacgcctct tccaccaacc agtggcacaa
    31441 cttcaacatc atccgccgca acggcgccgt ggtcggtttc gaacccagca agatcgccat
    31501 cgccatgacc aaggccttcc tggccgtcaa cggtggccag ggcgccgctt cggcgcgcgt
    31561 acgcgagttg gtcgaaaccc tcaccgcccg cgtggtgaat tccctgatcc gcaaccgccc
    31621 cagcggcggc aacttccaca tagaagaaat ccaggatcag gtcgaactgg cgctcatgcg
    31681 ctcgggcgaa cacgacgtcg cccgcgccta cgtgctgtac cgtgaaaagc gctcacaaga
    31741 gcgcgcccgt gacacgcagg cccaggctcc ggccgcgcaa agcacgctca acgtcaccga
    31801 caacggtatc cgccgcccgc tcgatctggc cctgttgcgc gccaccatcg ccgacgcggg
    31861 tcaagacctg ccccaggcca tcgacgccga ccgcatcctg aaggaaaccg tcaagaacct
    31921 gtacgatggc atccccgtcg acgaagtctt caagtccgcc atcctgtcgg cccgcgccct
    31981 gatcgaaacc gagccctcct acagccaggt ggccgctcgt ctgctgctgc acaccatccg
    32041 caaggaagtc ctgggcgagg aagtctcgca agcggccatg gccgaacgct atgccgacta
    32101 cttcccccgg ttcataaagc tgggcatcga gctcgaactg ctcgacgaaa aactcgccca
    32161 gtacgatctg gacaaactcg ctgtggcgct cacgtcatca cgcgaccagc aattcacgta
    32221 cctgggcctg caaaccctgt atgaccgcta tttcctgcac gatgcccacg gacgccgcat
    32281 cgaactgccg caaatattct tcatgcgcgt ggccatgggc ctggcgctca acgaaatcga
    32341 ccgcgaggcc cgcgccatcg aattctacga agtcctgtcc tcgttcgact tcatgagctc
    32401 gacccccacg ctgttcaaca gtggcaccct gcattcgcaa ttgtcgtcgt gctacctcac
    32461 caccgtgtcg gacgacctgg tcggcatcta cgacgccatc aaggaaaacg ccctgctggc
    32521 caagtacgcc ggcggcctag gcaacgactg gaccccggtg cgcgccctgc gcagccatat
    32581 caagggcacc aacggcgaaa gccagggcgt cgtgcctttc ctgaaagtcg tcaacgacac
    32641 cgccgtggcc gtcaaccagg gtggcaagcg caaaggcgcg gtctgcgcct atcttgaaac
    32701 ctggcacctc gacatcgaag aattcctcga tctgcgcaag aacaccggcg acgaacgccg
    32761 ccgcacgcac gacatgaaca cggccaactg gatccccgat ctgttcatga aacgcgtcat
    32821 ggaagaaggt gactggaccc tgttctcgcc gtccgacgta cccgacctgc acgacaaggt
    32881 cggccgcgcc ttcgaagccg cctatgtcgg ctacgaagaa aaagccgccc ggggcgagct
    32941 caccctgttc aagaagcttc cggcgacgtc cctgtggcgc aagatgctgt ccatgctgtt
    33001 cgaaaccggc cacccctgga tcacgttcaa ggacccctgc aacatccgct cgccgcaaca
    33061 gcacgtgggc gtggtgcaca gctccaacct ctgcaccgag atcacgctca acaccaacga
    33121 ctccgaaatc gccgtgtgca acctgggctc ggtgaacctg gcggcccacc tcaagcagga
    33181 ggccgacggc cggtacgtcc ttgaccacgc caaactcgag cgcaccatca ccacggccat
    33241 gcgcatgctc gacaacgtca tcgacatcaa ctattacgcc gtgcccaagg cccgcaactc
    33301 caacgtgcgc caccgccctg tcggcctggg catgatgggc ttccaggact gcctgcacat
    33361 catgcgcgtg ccgttcggtt ccgacgcggc ggtcgaattc tccgaccgct cggccgaggc
    33421 cgtctgccat tacgcctact gggcctccag caagctggcg gtcgaacgcg gccattacgc
    33481 cagcttcaag ggttcgctgt gggatcgcgg catcctgccg caagactccc tggccctgct
    33541 gcgcgaagag cgcggcggct atgtcgacat cgacgacagc agcacgctcg actgggacac
    33601 cctgcgcacc cacatcaagc agtacggcat gcgcaactcc aactgcgtgg ccatcgcgcc
    33661 caccgccacc atcgccaaca tcgtcggcgt gtcgccctgc atcgaaccca cctaccgcaa
    33721 cctgtacgtg aagtcgaacc tgtcgggcga gttcaccgtg gtcaacgact acctggtgca
    33781 tgacctgaaa gaacgcaacc tctgggacga agtcatggtc gccgacctga agtacttcga
    33841 cggcagcctg gaacgcatcg accgcattcc ggccgacctg cgcacgctgt acgctaccgc
    33901 cttcgaagtc gacccgcgct ggatcgtcga atgcgcggca cggcgccaga aatggataga
    33961 ccaggcccag tccctgaaca tcttcatgcg cggggtgtcg ggcaagaagc tggacgacac
    34021 ctacaagctg gcctgggtgc gcggcctcaa aaccacgtac tacctgcgct ccagcagcgc
    34081 caccaacgcc gagaaatcca ccggccgcgg cggcgaactc aacgccgtgt cctccggtca
    34141 aaaccctggg gtgtccgccg ccgcgaacct gcccgaaccg gagatcctgg gcgccgtctg
    34201 caccctgcgc cccggcgacg tggggttcga agactgcgaa gcctgccagt aaatccacga
    34261 acatcacaga aggcaagatc atgctgaact gggacgacac tcccctgcaa tcctccaccc
    34321 cggcgcccgc gccggccagg gccgagcgcc cggccgcgcc cgtcggcgcg gcaagcgaca
    34381 cccccattcc cacgcccgcc gccgcgtcca cccccagccc cgacgcccac cgcgtgcggg
    34441 cctcggaaaa acgcatcatc aacggcaaga ccgacgtcaa ccaactggtg ccgttcaagt
    34501 acaagtgggc ctgggaaaaa tacctggcca cctgcgccaa ccactggatg cccaccgaaa
    34561 tcaacatgtc gcgggacatc gccctctgga agaacccgga aggcctgacc gacgacgaac
    34621 ggcgcatcgt caagcgcaac ctgggcttct tcgtcacggc tgactccctc gccgccaaca
    34681 acatcgtgct gggcacctac cggcacatca ccgcaccaga agcccgccag ttcctgttgc
    34741 gccaggcgtt cgaggaagcc atccacaccc acgcctacca gtacatcgtc gaaagcctcg
    34801 atctggacga atcggaaatc ttcaacgcgt acaacgaaat cccgtcaata cgcgcgaaag
    34861 acgaattcct gatcccgttc atcaacgcca tcgccgacat gaacttcaag accggcacgc
    34921 ccgaagccga ccaaacgctg ctgcgttcgc tgatcgtctt cgcctgcctg atggaaggcc
    34981 tgttcttcta cgtgggcttc acgcagattc tggcattggg ccgccagaac aaaatgaccg
    35041 gcgccgccga acagtacatg tacatcctgc gcgatgaatc catgcactgc aacttcggca
    35101 tcgacctgat caacaccatc aagctcgaaa acccgcacct gtggacgccg gaattccgcg
    35161 aggaaatccg cggactgttc aagaaagcgg tggagcttga atacgcgtac gccgaagaca
    35221 ccatgccccg cggcgtcctg ggcctgaatg cctccatgtt caagtcgtac ctgcgcttca
    35281 tcgccaaccg ccgctgccag cagatcgggc tggaggcgct gttcccgcag gaagagaacc
    35341 cgttcccgtg gatggccgag atgatcgatt tgaagaagga gcggaatttc ttcgagacgc
    35401 gggtaatcga gtatcagacg gggggggcgt tgagctggga gtaggtgtca gacacagcat
    35461 caagtgactc tattcacacc aacaggtgac tttgagagta attcttattt ggaaaccgtt
    35521 gtcgtgttta aggccgtcgg gaggattgct ggcggctttt ttatttgcgt gggttttcac
    35581 cgagttatta cagaaataat cagggggaca cgatgctcac tcaaggaact tggcaaggcg
    35641 cgctaggaac ggcagctctt agtgtgatta ttttttcgac ctctatactg ctaagcttag
    35701 tcagtggttt ttttggttgg tactcgatat taattggaat attcttattt atcgggtttt
    35761 atgtgattat tacaatactt ccactcttac taaatcttag tagaagtttc tactggatta
    35821 tctttgcttt gttgataatt catatatcaa taaccattct ctcctttgct ctgaattatc
    35881 actcaacggg gttgcttggc tctaaaggaa aattcgcccc ttcattttat gactcgctat
    35941 attttagtat tacaacattc acaacactag gatatggtga tattcgacct ttaccaagtt
    36001 accgactgac aacttcactg gaagcattaa tcggtatggt gtccatggcc ttgggagcat
    36061 ctgtaatttg gttatggtgt caagaaaatc tgattccaaa ggagatagca tatttcgatg
    36121 gtaatagacg gcacaaaaca tcacttggca taaccagact acgtataaga actatgacag
    36181 gcaaagagca taaccttacg gactggtatt tacccgccaa acccggtgaa acgtaccgat
    36241 atgatgatct acgccaagag tggatctcaa cgcctgagaa aatcgaacct ctggaagatg
    36301 ccgcaattat gagtatcgac catcgagatt aagagctctt agcaatttat tcgcacctgt
    36361 ccaggcaaca tcaatattac ctaaattttc ttacatatac atcgcacgat cactcatgag
    36421 gcttaccgcc agggtgaaga ttacgtgtgc cgtgagtatc gacacggaaa gcaagtgggg
    36481 ggattacccc acttggcgaa tcactcgatc aatctggata cgcacatcca ggatatcgtc
    36541 aatgctttca aatatgaaga tcttaccgat gtgatcctct gtggccattc ttatggagga
    36601 ttggtgatca ccggggtggc cgtatgtcaa tgtgtgttca ccgggcgctt acgaaatata
    36661 accatttgat ttttaagcat ttcgcaagtc atttgattca atgcttgtca gtcgaacacc
    36721 gaaggttcac ttaactcacc tcccaccacg gagccagcac agcacgtgcc agcgtgtgtg
    36781 ggcgaatgtt aaacatcaag aaagcaggcg tcgactcgcg atcaatgccg atctgcgcga
    36841 catctagagc atgaatagct gtgtaataag tatgcttgcc gtgcccggcc ggaggcgcgg
    36901 cccccaaata gcgcttgagg ttggcatcgt tcgctaactg ccatgcacct ggcggcaggc
    36961 cgctgctacg ctcatcgccc gcgcccgacg cgagttgggt aatgctcaca ggtatatcgg
    37021 caacggccca gtgccaaaaa ccactggcgg tcggcgcatc gggatcgtat accgtgaccg
    37081 caaaactctt ggtgccgggc ggaaaaccaa gccaagagag ctgcggagac gtatcggtcc
    37141 cacccgctcc aaaaatgccg ctaacctgcg ccgatttcaa ccgaacgcca tcggcgatat
    37201 cagtactcgt tagcgtgaat gatggaacct ttgcaatgct gtcgtatgga tttgccatga
    37261 tcatgttcct tttctggcgc cacgttttac ttcacgggac gagaatcccg cggccaacca
    37321 ttcttccttg gtctaggtcg ttcagagcat cgcaggcagc ctccaacgga taaacgttgg
    37381 tatgcagttt caccctcccc ctggcggtaa gagtcataag ctcgaccaaa tcgttgtatg
    37441 tacctaccag attaccgacc acactgattt ctcgcgaaat gaaatcgatc gtaggaagtt
    37501 tgatcatgcc gccatagccg atgacataat acgaaccgct attgcgcagg attttggggg
    37561 cgagcgcctc ggtgccatgc tccgcaacgt agtcgaacac cacatgtgca ccgccgtccg
    37621 tgatatccaa cacctgtttg gttacggcat cgtcgtcgat cgagacaact gtgtgatcgg
    37681 cacccagttc gcgggcaagc tctagagccg cctcggaacg gtctattaca gtaatttcgg
    37741 cggcagtgat ggctttcaac gcctgtatgc cgatatgacc gaggccgcca gcgccgatca
    37801 cgacagcatg gcttcccggc cagagaagat tcttcgcttt tcgtaccgca tggtaggcgg
    37861 taatgccggc gtcggccagt gcggcgatat ctcggggttg cagactggaa tcaagcttga
    37921 caactgcccg ggcattggtg ctcatatact ctgccattcc tccatcgaca ttgagccccg
    37981 gaaaatcgga gtgtaaacag tgcgaatcat ctccgacacg acacgctggg cataagccac
    38041 aactcgccaa cggatgcatg atgacagtgt cacctacggc gacattgctg acagcactac
    38101 ccaccgcaga aacccagccg gcattttcat gcccaaggat atagggcaag cgcggatgct
    38161 ggatggcctc ccactgcccc tcgataatgt ggagatcggt tcggcacaaa cctgctgctc
    38221 ccaccttgac gatgacatcc cacggcccct tgacggtggg ctcatcgacg tcgtcaatcg
    38281 agggttgttc tttataccga tgcaggcgta ctgctttcat ttcttctcct taatggcctt
    38341 ttcgcccgac ccgccgccgc ttcgtagtca aagccgcctc taccgttatc gcggatcacg
    38401 tgcggctgta gtgttacagc tctcgcctgg acatctggcc tgcgatgtaa tcggcctgcc
    38461 gaattgccaa agtaacgatc gtcaacgtcg ggttttctgc cgcgctcgtg gtgtattggc
    38521 ttccatcgga aataaacaga tttcgaacct cgtgtgcctg cccgtgcgcg ttacagacgc
    38581 catcgtccgc cttggcgctc atgcgacagg tgcccatgtt atgcgtggac gggtaaggcg
    38641 gcacccgata gacccgcttg gctccagcgg cctggtagat tgcctcacct cgcttgtatg
    38701 cgtgctcgcg catggccttg tcgttcgcat gatccgtgta gctcacaacg ggaatcggca
    38761 gcccatgctg gtctttctcc gtcgcatgaa gcgtgatgcg attgttggcc tgcggcatat
    38821 cttcgcccac gatccacatg ccagccatat gtgtatagtt atccatggcc tccgcgaatt
    38881 cctgccccca tgctccgggg ttgagaaatg cagcggtaaa cggcaggccc agcgcgatcg
    38941 tttcgaagtg atacccacca gagaaaccac gtttggaatc gttcactttc tcgtcttcga
    39001 caatgccggc cataacggtg cccttgtaca tgtcgacggg tttctcaaag actccaaaca
    39061 cggagccggt gagatgcgac atgtaattac gcccgacttg gccggagcca tttgccaagc
    39121 cttgcgggaa gcgtgctgaa tgcgagttga gcagcaacct gggtgattcg atggagttac
    39181 cggcaacggc gactacccgc gctttttgcc tctgctcatg cccgtcctta tcgaagtaga
    39241 caacactcgt cacccttccc ttgttgtcat gctcaatgcg ggcgaccatc gcttcagccc
    39301 gtagttcgac gcgtccggtg gccaaggctc gagggatttc cgtgaacaat gtcgaccatt
    39361 ttgcctcata gagacagccc tggaaacaga acccgcgctg gtggcagaag gagcgcccgt
    39421 ccgtcgcgat gctgttgata gccatgcgtc cagtactgca ttccttgtag cccactttag
    39481 tcgccccatc atacataacc ttgaaattgt tatttccggg caatccggga aggttattcg
    39541 tgcgggtgac gcgcatcttc cactcggcct ttttgtagta tggctccatc tcttcgagtg
    39601 tgatgggcca gtccagaagc gtcgtgccgg gtaaggcgcc ataggtagtg cgggctttga
    39661 attcatgctt ttgaaatcta agcgaagcac cagcccagtg cacagaggtt cctcccaccg
    39721 ttttacatat ccaagcaggg agattcggga agtccttcgc aatccgccaa ccacccgaag
    39781 cgacacgctt gtccagccat gcgagctggt gaaacgaggg ccattcatcc gatatgaatt
    39841 ctgcagaggt gtgtagtttt ccagcttcga gcaagacaac gtctatcccc ttctgagcga
    39901 gttcatttgc cagcgttcca ccgcctgcac ctgacccgat gatcacgacg acgtgatcat
    39961 cgtcgtgaga aaatgttttc tgattgtcat acttggccat gattgtctcc actgtggttt
    40021 ctgcgattag ttcggtacgg ggccactcgc cgacgctggc ggcgacacca accatttcaa
    40081 gtcgttgaaa ccacgatgta gatagcccgt cgcgcccttg ggtccggggt aaccgaaatg
    40141 cgcataagcc agttcgttac tgtagagtgc cacgacagcc gtgcttcgta ccttctcgaa
    40201 aaacggtgtg cccgcaagcg ctttgacttc ggcaaactgc cgggcgccgt ccgcactgag
    40261 ccaatttcct ttggcagtct tgtccatatt tgcgacaccg tcggccagca gcttccgcag
    40321 gctctgatct ttagccgcat cctcgtcgag gttcttgacg accagcgcat agactgcgtc
    40381 ttccaacgtc ttgtgcggat acagatatcg agtgaatcga agtagcgttt tcccctgatg
    40441 cgagtccaga gtgtcgagct ccagcgccca ggtcttagtg ggagcaagca ttgctaccgc
    40501 accacttgtt gcagcgaggg taccaacaat tatcgacccg gtttctttga gaaacctgcg
    40561 tcttgagctg gaactatcga ccatagccgt ctcctattac atagtgtggg acattctcag
    40621 atgttctctg cgctcgcagg gaaacgttgg cctcggattt ctggcgcgcg agcatacgtt
    40681 ctttttataa tcttgcggga gaaacgcgtg gtagtatcgg cctactacgg actctccagc
    40741 cttgattagc caactcggaa atgcacattg aaacccatgt cgggcttgat ggcagaggct
    40801 cttacagggt ggtgggattc atgttctatg ttctagttcc catcctctgc gaacgacgcc
    40861 cgagggcaag gggcaaccag tcagagctgg ctcctactca tgtgtcgatc tattcgatgc
    40921 gttcgggcac caagcgctgt ccacggacaa cctccacaca agacacgcgt agaaatgagg
    40981 cgaagttact ggcacatccg ctctgcatat actcaccgta aagcaccgcc aaaagtaatc
    41041 ccacggacat gccctggtct tcggccaact ctttcagaat cacccagaac tcttcttcca
    41101 gacccatact tgtaacgtac cccttgatac gtacggatcg agtctcacgt tcaaaaaaat
    41161 tggacccgat tgacttagag atttgacaca tatgcgaatg cctccaccac ttggttctgg
    41221 aaacaatact actgtgtttg ggtcgtgaaa acgttgtcct tgctaccgat caacctgagt
    41281 ttgtttttat gagggccccg gcgttcggtt gtattcgtaa tatacgcttt tgtcaatcag
    41341 gctctattca cctttagccg acctttttgc cgcatacgat agccagttct ccggtgccta
    41401 caccgtgggc ggacgcatac agcccctgca tctggcaagg actgagcggg tcgatgatca
    41461 gccgcgccgg gattgccggc acctggtctt cctagatgaa acggcgacga ccaccaacac
    41521 gacgcgacag tagggacgtt cactgttagc gccggtgtac tactgccccc catgtcagaa
    41581 tcacttgatg tgatacccgt cggtagctca aaagtggatc aattatcggt cggcaacaac
    41641 ggatgaggtt tgtcggtcgt agagggttcg ctcgacaaat ccttcaagta gtcgatgagc
    41701 catcttcccg ctggcccggg tggcgctgac gctcgataac aggcggacat tgtcaacgtg
    41761 aagtctgctc gcacatcgat gagcaccaga cttcccgcag cgacgtctga atccaccatg
    41821 tgacgcggca tgctgcccca cccaacgccg tccttgagaa acgcgtactt ggtcgaaagg
    41881 tccgccagcc gccaagtcga tggggaaaaa acgccatagt cccggccagc catcagatcc
    41941 gaccggtcgg tgaggacgag ctgcacttgc ttcgccaact cgcgttttgg gatccggccg
    42001 cgaaaaccgg cgagtggatg gctcggtgcc gccaccatca ccaaggggac ctcgttcacg
    42061 ggttcgctga ccagcgatgg gaattcgacc ggcaacgacg ccaggatgcc caagctgcaa
    42121 cgttcgtcca acacgggctg gtacgccgcc cccaaccctt ccacgaaaag ccgtaacggg
    42181 gtcaatggga atcgttccgc gaacgccttc gctgccgcag cgattgtggt cgccggaaag
    42241 aacacatcga cgaccaccga caattcggcc tctagccctc cggccataag cttcgcgcgc
    42301 gccttcatcg catcgacacc cgaaacgatg ttgcgggcgt cggcgagcag cagcaggccc
    42361 tcgggcgtga gcttcgggaa ccgcgccgtg cggtcgaaca gcacgacgtc gagttgcgcc
    42421 tcgagaccgc cgatccagcc gcttaccgcc gattgcacac gatagagctt gcgcccggcg
    42481 gctgagaagc tgccctcatc gactgcggcg atgaaggccc gcagttgatc cagtgagacg
    42541 ccgtccagca tgagcaccca tcccaaacat agatagttcg aatctcattt tatggtcttt
    42601 tcagatctta cgggaagtct cactattgat gccagcctaa tcacacatgg caccgacatg
    42661 aacctttcag gccttgacgc cagcggagaa caatcatgaa cacgcaatac agcatcatag
    42721 ggtccggccc tgttggcagg acattggcag gtcttttcac gaggggcgga atcgatgtgc
    42781 agttggccaa ctcgcgtggc ccagaatctc tggcggcaat cgccgccgag ctggggcgac
    42841 acgtggttcc ggtcacgctg gagcgcgcac tcgacgcgga cgtcgttttc cttgcggtcg
    42901 gattcatgca ggtccagtcc gtggcgatga aacgccccga ctgggccggc aagatcgtag
    42961 tcgacgtgac caacgccttg cagcccgccg aggtacggga aacgcaactg cgtggcctcc
    43021 tgtcgtccga gatcaatgct ggtttcgtgt cgggcgcaaa gctggtcaag gccttcaatc
    43081 acctacctgt acagcagctt gggacgaatc cctcgctaag tggacagcgg caggtggtct
    43141 tcgtagccag cgacgacgcg ggtgccagca cagcgatcgc ggcgcttgcc acgcgactcg
    43201 gcttcgctcc tgttgaactc ggaaggctcg atcagggcgg tgcggccgtg cacgttctgg
    43261 atggcaagcg gggcggcctt ttgtttcaga acctggtgaa actcggctag ggaagagacc
    43321 catgaacgag caacgaaaac aacagtagtg aggtttgtca tgatcattga tctgaaaggg
    43381 cgaaaggcca tcgttacagg cagtaccgct ggcatcggcc gggcggttgc ggaggggctg
    43441 gcccgcgccg gcgcagcggt cgtcatcaat ggccgcagtg aggagcgagt cttcgccgcg
    43501 ttgcgagaga tgcgcacgct ttttccagcc gccgatatca ccggatttgc cgccgatctg
    43561 gcaaccgcgg agggcgccgc cacgttttcc gcgcgggcga cggatgctga cattttggtg
    43621 aacaacctgg gcacggctcg accaaagccc ttcctcgaac tcaccgatag cgactggctg
    43681 gacatgttcg agatcaacgt gatgagcggc ataagaatga cccgccacta tctgccggga
    43741 atggtaagcc gcggctgggg acgcgtggtc ttcatcagca gcgagtccgc gcttgccatc
    43801 ccgaaagaca tgatcgacta cggcatgacc aagaccgcgc aacttgcaat ttcaagaggt
    43861 gtggccgagt cggttgccga gacaggcgtg accgtcaatg cgatcattcc cggtccaacg
    43921 cgctccgaaa tcctgtcgaa ctggatgaaa gcgcaagccg aatccgaagg catcacgcaa
    43981 gaggaagccg agcagaattt cgtgaggaca atgcggccga cctcgcttct gcaacgtttc
    44041 acgacaaccg atgaggtcgc aaacatggtc gtctatgtct gctcggaaca ggcttccggc
    44101 acgactggcg ccgcgctgcg cgtcgacggc ggcgtgctca ggcaaatcgc ttgacagcaa
    44161 agcgcaagag gcagtgacat ggtacgtggc atcatattcc acacattctc acacatatcc
    44221 attgacccac accttcaatc gatggctgga accgtgctgg aacacgactg ccgttatggg
    44281 ccacgcaata gctggcaccg agactgaaga atgggaaaca atgggtgagg agcttggtct
    44341 catgcgggag atctccataa agggattgga tgcgtcactc aggcttcttc gatcgccgga
    44401 cgccttggcg cgaacaggcc atgttcctcg gggtggcgtt catgccgggc ggcccacatg
    44461 tagaacaggg cgaaaggcac aaacatgaac agcccataac cgacatggat attcgccccg
    44521 cttgagaaca ccagcgggta ccccattatg tgattacttg accgatgcta catagggttt
    44581 gaagccgtag cgtttgaaga tggccaagcc ttccggtgag cggatgaagt ccagccagcg
    44641 gtgcgcggct tgggcgtgag gtgtgccctt gaccaacgcg ccggcgtaga tcgcagtcgt
    44701 gttctgtgca gtcggtatat cgacgtggct gatcggatga ccggcctgtt cctggaacat
    44761 ggcttcggac tgccaagtca cgccgccttg aaccttaccc tgcatgatcc acaagggtgt
    44821 ttgccgatga tggatgtggg tgagttctgt cgtgccttgg gccaccttgg tctgatagac
    44881 catcgtcttc agagactgac cgcctgcctt gaccagcgag gcctcgacct gccgagcgat
    44941 accctcgaac ttcgggttgg gcatcgccaa gcgtatgtct ggcttaccca gatccgcgag
    45001 gcctgtaatg tgcgccggat tgcccttggg caccatgatc gtcaaggtgt tggtgacata
    45061 aggcatggcc ggtgcatcga gcaacccggc tgtgatctgc ttgtctacgg ccttgagacc
    45121 agccagatag acgtcgggct tgacggtcca tgtcatgttg cccaccgtca cggtgcctcc
    45181 ggccttgatc tggcgcacca gcaacccagg tggaatagtt tcccagtaca ggcgcccctt
    45241 gtattcggga tacttgtgct cgaaggcacg aaccaagggg gacatggcaa agaagtaatt
    45301 accgccgacg tacagcgaca gcacagggtg aaccgggtcg ccatgaaaat cggcaaggtt
    45361 gtcagcatct ggcacggtga actcgaagcc gcgatgggta gcatcattat tcgcgccgtt
    45421 ttgccagggc ggcgtgacgg cggcccggtt aacttgatcg gtcggagcgg catgcgcagg
    45481 tgcggcggtt cccgccatcg cccaaatcag acaggcagcc aatgcagtga tcttcgacat
    45541 agtgaattcc tcagaaatgt ttcaagcgcg tccagaaatg ggagcgcttc tcacagctcc
    45601 atcagatcgt agccacgatg ctcaagcgtc gtcacggtgg ttagcccgga cagtgcgagc
    45661 gtcacactgt ggtctaccca accgtagtga aacttatgct cagccacagc ctgcgcgcag
    45721 acggctacgt ccacgccggc cttgcgcagt ttggcaatca gcggcaggtt ggggttgtct
    45781 acgccataca cagccctgta gtccttattg tccagcgcca agggcgtggc ggcgccgtag
    45841 gccacggcga caaacttcaa atgatccaac ggcacacctg aggcgacgta aaggttcact
    45901 gtgcgcgcca cgtgatcaag cgcgggattg acttgggtgg gcgtctttgc agcctgggtc
    45961 agtgcaaaca cgatcttgta ggtctgattg gcctgcggac ggtaggcggc gtgggaaacc
    46021 atgtggatac gcccgtatcc ctggatagtc ggggtggtcc agaaggctcc gggagactgg
    46081 gcatgactgg caacggaggt ggcgagcagg acgccagcgg cgaggagcgc ggtgagcttg
    46141 tgcatggaat aactcctttt catatgtgta gaaaacccaa cgaggagtgg tattgattgc
    46201 ggctgttttg ccgccatgct cggcatcacc tccttagggg acacacagta tgggcatccg
    46261 cgccatatac atccaataca attaaatact attattcatg cataaaatgc atatgaatct
    46321 tcatcatctg agtgtgtttc acactattgc gatcaccggc agcatcacag cctcctccca
    46381 acagctgcac atttcccagc cggcactttc gcacgagttg aagaatctgg aggatcggct
    46441 tggtgtggct ctgttcgaac ggctgcctcg gggcatgcgg ctgacgcatg caggcgaagt
    46501 gcttcgcgac tatgcggatc gcctgttcga gatcgcgcgt acggccgaat cggaaatgcg
    46561 cgccatcgct gaggctcgca agggggctct caccctcggc gccagcaata cgatcggcac
    46621 ctacgtgctg cccagactgc tggcgcattt tcgccgcgac aatccggacg tacgtatctc
    46681 attgttcgtg ggcaataccg cgcagatatc gcagggggtg gcggacatgc gattcgcgct
    46741 tggtttcatc gaaggcccat tgcacaccga cggtttggtg gcagagcgct tccaggacga
    46801 tgaactgttg cccgtggtcg ccgcgggtca tcctctgttg agcaggaaac gcctggggat
    46861 cgacagcctc gacggagagc cactgcttgt acgcgaagcc ggctcaggca cgcgggagtt
    46921 gttgatggac atgctgcaag tgcaccaggt aaaactgggt agcatgatgg agttcggcaa
    46981 caccgaggcg atcaaacagg ccgcgatcaa cgggggcggc attgcatggc tcccccgcat
    47041 cagcatggcg cgggagttgg aaagcggtgt gcttttcacc ctgccggtca agagtctggt
    47101 gatcaaccgc cctttccgtg tgatacgtcg tccccgcgcc cattccagcg cggtgatcga
    47161 cagcttcctt gctctgctga gtctgcaaaa gcagcccaaa aaatctgact gatacattga
    47221 atgcgtgaca tcgcgatacg ctggctgtgt tgtcatgtag cccatatcca gaacccattc
    47281 atatctcaga cttccgtata gccaatcaga agtgtggcat catgcgcatc gcgagattcg
    47341 acatgaccca caacgtgccg cccaccatca cgatcaacat gagaatggtg aaaagcagga
    47401 ccttgaattc gtcgccgctg tgttttgtcc caatgtgcag gaagaaccga aagtgaaccg
    47461 cggcctgcaa cagggcaagg gcgccaatcg ccatgaacag cgtgtgttgc ggcaggcttg
    47521 gccagtagtg aatcaaagcg aaggcggcca gtgtcagaat gagcgcggca attagcccag
    47581 tgatgtaggt aaagagttct tgccgttcgt cggcggcggg ttcgacgact tgcgtgtcat
    47641 ggttggccat catgccaccc ctgcgatgta aacaatggtg aaaatcccga tccagacaat
    47701 gtcaaggaag tgccagaaca gagccagccg ctgaatgccg agccggctcc agtgatccag
    47761 gccatagcgc cagatctgag cgatgatgca taccatccac acgcatccca cgaatacgtg
    47821 caggccatgc agcggcacca gatcccagaa gctggaaaga aagccgctgt tctgggcggc
    47881 accacccaag gccaccatcg agaaaaaatc gtgcagttca agcgcgagaa agcccgcgcc
    47941 gagcaccagc gtgacaagca tccatcccag cagtcgcgtc agactgtgct tgtacttcaa
    48001 ggcaagcgaa gccagcccga aggtaaagct gcttgccagc agcaacaggg tttgcgcgaa
    48061 cacgctaccc atgtcataga gcgtgcgtgg ccccgggccg ccggatgtgg cgtgccggct
    48121 gaacagatag gtcgagaaca ccagcgcgaa caggaccgcg tcgctcatga ggaagaacca
    48181 gaagccaaac atgatggtgc tggattcgtc atgctcctcc tcgggctccg ttgccatcct
    48241 catcccagga tactgaaaga cgttgtctgc catggttcat ctctccaatc gaatttatga
    48301 gcggcgtggg gcctgagggg ccaggtcgag acgcgaggcc ccacgcctgc ccgccgcctc
    48361 ggcggcattg acgcctgttt ccagtgcggc aactcccaca ttggcgggcg agaactcctc
    48421 ggcacgggtc accggttttg cctcacgcac gtgtgccagc cacagcaagg tttccttctc
    48481 caccacgctg gctggaatca ctcttgtcat gtgatgcacg aacccaaacc gcaccatgaa
    48541 cacgaccgtt gcaatggctg cggcgatgac cagccaccaa atgtgccaca ccagcccgaa
    48601 agctagcaga aacgtggcgg cgccaatttg cactgccaga gcgttgttct tgggtacttc
    48661 gatgtcgacg aactcctgtg gcgtcgagta ggccagcccc gcttccttcg ccaccgcgaa
    48721 ggcgtcacga ctattgatca caggcacgac cgggaagttg tactcgggtg gaggcgccga
    48781 agtggcccac tccaggctgc gcccgtccca cggatcgccc gcaaacacct ggttttccgc
    48841 acgttgtttc cacgacaccc acacttgggc gagcagcagg aagaaggcga tgttcaacaa
    48901 cacggctccg acgaacgcca ccgtctcata gggaacatac gccggttggc tataacccac
    48961 catgcggcgt ggcgcgccca gcagtccgag cgcgtatagc gggaagaacg cgaggatgaa
    49021 cccaatgatc cagcaccagg ccgacgcctt gccccagcga tcattcagac ggaatccgaa
    49081 taccttcgga aaccagtatt caaacccggc aacgaggccg aacagcacgc caggaataat
    49141 cacattgtgg aagtgcgcca ccaggaacac ggtgttgtga acctggtagt ccaccgtcgg
    49201 attcgccagc aggatgccgg tcatcccgcc gatgacaaac agaaagataa actgaatcga
    49261 aaatagcatg ggggtggtaa agcgcacgcg cccccggaac atggttgcca tccagtcgta
    49321 tatcttcaca ccggtgggga tgccgatcag catggaggca atgccgtaga ccgcgttcac
    49381 gtccgggccc tggcccatgg taaagaaatg gtgcacccaa accaggaacg aggcgaaggc
    49441 aatgcacgcc gtggcaaaca ccatcgattt gtacccgtat agcgtcttcc cggagaaagc
    49501 gggaatcacc tcggaatata tgccgaacgc cggcatgatg aggatgtaga cttccggatg
    49561 tccgaacagc cagaacaggt tggcaaacga catcatgttg ccgcccatgg cgttggtgaa
    49621 ataatgcagg cccaggtagc ggtcgagggc cagttgcccc atggccacgg agagtggcgg
    49681 catggcgaac accagcatga ttccggtgac cagcgacgtc cagctaaaga gcggcatagc
    49741 catcaggcgc atgcccggtg cacgcttgcg caggattgtg accacaaaat tgatgcctgt
    49801 catggtggaa ccgatgcctg acaaagtcag tgcccagatc caataatcgg gccccacacc
    49861 cggctgccag gcggtttccg tgtacggcgg atagccaaac cagcctcccg tcgagaactg
    49921 cccgaatacc agtgagatca tcagcaaccc ggcgccggcc gcagtcaacg agagactgac
    49981 tgcgttgagc gtgggatagg ccacgtcgcg cgcaccaatc tgaagcggca tcacgtagtt
    50041 gatgacaccc acgaagaacg gcatagccat gaaaaagacc atcagcgtgc catgcgtgct
    50101 gaacagctcg gcgaaatgcc cgggggtgag aaagccgccg cccagccccc acgcctgctg
    50161 tccgcgcatc aggccagctt cgagcagccc acgtgccagc atcaccagcg cgagcaccac
    50221 atacatgatg ccgatcttct tgtggtccac gctggtgagc cattcccaga gttcgctcca
    50281 tttgtggtga taactgatga gcgcgatggc agcgacggca ccaaacacca cacaccctgc
    50341 ggcaaacgtg gcgatgccac cgttcaccac attcacggta gtggggttgt ggaacatttc
    50401 gtagaacggc agcgagctcg cgccgaggcg ccctatcgaa tgactgaaaa tggcagcagc
    50461 atccatttct atgactccgt cgttgatgcg aaaaccggcc cggcttcagg gaatggcggg
    50521 cgcattggct gacacgccgt tcatttgagg gaatttgacg gttgtgcctg atgtggttgc
    50581 gtgaatcacc tggtgaaaga gccctgtcgg gacatcacgc aggaagattt cgccatcacg
    50641 caccgtactc gaaggcaatc gcagcgcgct ggccaattgc cctttcgtgc cgcgttgcga
    50701 cagcagcgcg tacgtgtgtt gatcaagtgg cacgccgtac gcctgaacct gcttgaccca
    50761 ggccttgaac gcagttggct tgagcgccaa cgcggtgaag gtctgcctgg cgaaacccga
    50821 cccgttgtac atggtgttca tgcccagcga gcgacccggg ccgttggcct cgaagtgtag
    50881 ttgcgtcacc atgccgccca tggcgtagat ctgcccgatc aatgcgggga tactcaacga
    50941 ttgcatgacc gtcgcggagg tgagcttcat ggcgaccggc tgtccggctg gaataggcag
    51001 cacgccaacc gtggcgatct tcagcttcgg gtagaggaac aaccacttcc agttgtagcc
    51061 gatcgcctgt acctgaagtg tcggctgtgt cgacaccagg ggccggaagg ggtcgagcct
    51121 gcgcgtgttg cgccatacga agacaccaag aaacatgacg attgcaaccg gccccgacca
    51181 cgtgagtacg ctcagtacgg agttgtgcgt ccacttgggc tcatagcgcg tgtgcgcttt
    51241 gttgctgtag cggtaatgcc aaagtatcca gatcgtcaga atgtatacgg gcgctgcaac
    51301 gagcaccgtc ataatcagga cgacgaacca gaaatgccag cgttccgcgg cagcgatcgg
    51361 cccctgcgga tcgaggaacg agagatggtc gtgcaagttg cgcaaaggtt cacgcgcaca
    51421 acttgagagc acgaaggttg agccaatgat ggagaagatc cgcagcgcga gccacgcgcc
    51481 gggttgccaa tgaaacttgg gtgcggcgtc aagcacgccg gactgctgag gagttgaatt
    51541 cataccgtac acgaccgtgg tgggaaacca cgcgaatgct cggggcgttt gcggatccgg
    51601 acccctcagc cagatcacgc ttccaacttg cgatcgtgta ctacgtattg attgacatca
    51661 acttccgcga acgggaggca acctcctgcc gccatagcaa aacacaacta ccggtcagac
    51721 gcaactacat ttcagtgtga catcgtgcag ctttttcgcc ccttgcaacg cgcttgcgtt
    51781 ctcgcgaacc gctaaagcca aactgttcgg gcgagcagct tcaaattcta cgccgcttct
    51841 caaaattcgc cttaatgcca ttattcataa gtcaaaaatt cgtcgcgcga cggcgttgtt
    51901 cttgctgagt tgcgcctgac catccccatg gtcgcaacct aatgagtcga ggcaacagtg
    51961 aattggctac gccgcatgtt cacccggctg ataatgccct ggttcggaac ggaagggaat
    52021 ggccagccgg ttccatccgt taatggcgat aatggcgaat gataagtcgg tcaattcctt
    52081 gtcattgaac tgcttgcgca cttgctcgta gagggcatcc tcgacggggc cgtctgagat
    52141 ttttgtcagc gcctcggtcc atgcgagggc cgcgcgttca cgctcgctat agaacggagc
    52201 ctcgcgccac gcactcaaaa cataaaggcg ctgttccgtt tcgcccgccg cccgggcgtc
    52261 cttcgagtgc atgtccagac agtacgcaca gccattcatc tgagatgccc gcatcttgac
    52321 gagttccagc agggggtgct cgagcccgga attttccaca tactgctgca aattgcgcat
    52381 cgccttgaaa ccatcgggcg aagcatgaac ataattgaga cgttgtgaca ttttccttac
    52441 tcccgctgat gactaccgtc accgataaat tttgaaccgc tcacaaagtt gtaagtagtc
    52501 caccgtattt tcaaacccat cgatctcttc gcgcataagc cgatcggtcg cattgacgaa
    52561 accgctccag gccgccagcg caagggtgct tgccgtgctg atacgacgca ctccgatctc
    52621 ggcaagcgtc ttgaacccga gtttggccgc gtgccccatc atgatgttga tcggcttcgg
    52681 ggccaccgcc tcgacgactg cgctgatctc ttcacgcctg gacaggcccg gcgcgtagag
    52741 gcagtccgca cctgcgtcag catacgcttt tagacgtttt atggtctcgt cgagatcggg
    52801 acgtccaacc agataacact ccgcacgtcc caccagcatg acgttcttgt ccacggagtc
    52861 aatcgcctcg cgtgcggcgc gcatgcgtgc cacggcctcg tcgaaatcat aaagcgcgcc
    52921 ttcgctgcct gctatcgagt cttcgatgga caatgcagac acccccacct gaacacacgc
    52981 acgcacattg actttcagcc catcaatggt ggaggagtat ccatcttcaa aatcggcgtt
    53041 gacgggaagg cccgtcgccc gcacaagctc accgcagtga cgcacgacct catcaaccgg
    53101 aatctcacga tctgggatgc ctcgcgagaa agcgtagccc gcgcttgtgc tggccaacgc
    53161 tttgaatccc gtctgctcga gccaacgggc cgtacccgga tcccagggat tgggaagaat
    53221 gaaacaccct gtctcgtgga gtttgcgaaa tgcggcgtgc cgcgccgaaa gagttgttgt
    53281 ggtcatagcg aaaatttcca aaaagttgac accggcgaac aacgaggaag ggtcgggtgc
    53341 aagccgcagg cctgcgttgc gtgtcgcggg ggtgagcgcc cacacgccga tcacaatccc
    53401 gttgtagatc atattcacgg gattcaccac ccagaaatca aaaccttgga ccaaccgcga
    53461 agcagtaaag ggcgtcttgg cacgccggat gtgacgaaag aaagccatac tggccattct
    53521 gtgcttttgg tcgctacccc ctagacatcc gccgccggta tatgctcttg gctttcccca
    53581 caagatcaaa ccacaaagat gcgacattcc gcccccaaag cccgtctgcc acgcaacgtg
    53641 tgcctgctga tcggtgcgcg cgcggtgcgc agtgtcgggc aaggggcgct ggtggccgcc
    53701 ttcacgcttt acctcgatgc gatggggtgg tccgctgcgg caatcggcgc agtgctgatg
    53761 agcgcactgt tgttctcgat cgttctcaca gcaatcgccg gtccgttgag cgaccgcggc
    53821 catcggcgta cgttcctgtt ggggtatgac gtggtgcagg cagtcgtagc catcgtcgcc
    53881 ttgaccacca cgacgccatg gctgttgatc ggcgcggccg taatcggcgg attcgggcgt
    53941 ggcggcaatg ggtcggccgg acccttctct ccaatcgagc aggcatggct ggccatagaa
    54001 ctgccgatgt cgagacgcgg cagtgtgttc agcgtcaaca gtgctgtcgg tctgatcggt
    54061 atgggaatcg gtgcgctcct tgccgcgttg ccaaccttgt ggatcggcca tgcaatgagc
    54121 acgcaagact atcggctatt gttcctattg ccgctggcag gcgctctcgc gagtttcgcg
    54181 ctgctatggg ccgcacgcga gcccaatatt gtggtgcgtg ccgatcgtga tgaagcatcg
    54241 ctgcgcacgc atcgggcgga aaaccgcctg cttgccaaac tgatgctggc caataccata
    54301 aacggcttcg cgatcgggtt gaccggtcct ttgattgcct attggtttgc catccggttc
    54361 caccgcgggc tgctcgaaat cggccctggc cttgccattg cgttcctgat cagtgccgcc
    54421 ggcgccttca tggcgggcgc gctcgcgcgc cgccacggca tcatggccac ggtgatctgg
    54481 atgcgcggcc taggcgtgct atttctgttt gcgctgcctc tggtgccatg gttctggctg
    54541 gccatgctgc tttacattgc ccggggagca gtcaaccggg ggaccggtgg cgtacgccag
    54601 gcgcttgctg ccgggcttac ccgtgtcgag cggcgtggct tggcctcaag cctccagaac
    54661 atctcccttc aactgccgcg cgcgattggc cccgccatcg gcggggtgct gtttcatgcc
    54721 gggtacttgt cgttgccatt cttgatcgga gcagtgttgc agcttgtcta cctgatcctg
    54781 tatgcccgct ttttcggctc tcatccggcc gcacaggcac atacatagca gccggaaggc
    54841 cagcctatag ggaagcgcca tcggcagcgg gtaaactggt catgcgggtt cattggatcc
    54901 tccaggagaa agacttgcct tcgacgacgc accaacctat tgattcgccg ctgacgcaag
    54961 cggcgatgtt cctcgtcctg accatcaatt ccgaacccgg gcacctggat gccgcgcgtg
    55021 cattttgcgc ggatcttacg gggctgcggc gatcggtcgg attccgtgat cctgatggca
    55081 acctgtcctg catcatgggc tttggcgccg atgcctggga tcgcctgttc ggctcgcccc
    55141 ggcccaaagc gctgcatccc ttcaaggaaa tccagggcgt gcatcatgcc gtatccacac
    55201 cgggcgatat tcttctgcac atccgcgcca accgcatgga cctctgtttc gaacttgcca
    55261 cacaaataac ggcacggctg ggggacgccg ccaccgtggt ggacgagacc catggattca
    55321 agtatttcga ctctcgaaat ttgatcggat tcgtggacgg ctccgagaac ccgaccggcg
    55381 tggaagcgac cgccgccacc gtcattggtg acgaagacag cgctttcgcg ggtggcagct
    55441 acgtgatcgt gcaaaaatat ctgcacgacc tggccggctg gaacgcgctg ccggtcgaga
    55501 tgcaggaaaa aatcattggc cggggcaaac agtccaatat cgagctggat gacgacgtca
    55561 aaccgacctg ggcgcacaac gcgctgaccg tgatcgaaga agatggcgaa gagctggaca
    55621 tcgtgcgcgg caacatgcct ttcggcgacg tgggcaaggg cgaattcggc acctatttca
    55681 tagggtacgc cagaacgccg cgccgcatcg aacagatgct ggagaacatg ttcatcggcc
    55741 gcccgcaggg caactacgat cggctgctgg acttcagccg cgccgtcacg gggtcgctgt
    55801 tttttgtgcc atcggccgac ttcctggacg atgtgcccga gggtggtgcg ccaagtcctt
    55861 gaaggcagtg aggcgacatg cgcgggttat gccacgctgg ttttttgggt ggtgacggcg
    55921 gcctgggacg ctgggcgcgt gtcatgaatc atttcttgca gagtatcgta tgctccggag
    55981 cgtcaaggca tccatggcta tctatcgcta tggatacact tccgtgcgga ccggtaagtt
    56041 gaacatggtt cccattgatt ttatatgtca atggctcttc cccctcacgg gccagaacat
    56101 tcacctgggt gccggacaca aaacgcaatg atccggccgg cactttgtcc ctgagcgcct
    56161 gaggcagtgg cgccttatgc gacccgttgc aataggcctt accggtcaca gtcacggttg
    56221 cgtttggcgg cacgctcggt ccgtgcgcac cggagcgttt aagcttaagc acggcatgat
    56281 caaaggtaaa tgcctcggcc ccctcggcag tggcgatcgc aacggagccg ccgaccaacg
    56341 tgttatccga ggaaatagaa cctcccttcc cgtcgacggg atcaagcctc acgtcgacat
    56401 tcgttgtact ggggggcttg ccgtatgcct gaatcgacat atcatatttc ttgccgttat
    56461 acgtgacggg gccgaggcca tatttctgaa acacatcctt gggtaaggcg taaaacgata
    56521 ggatggggga gccggcgcgg gccgatgtcg ttgcgcccac atggccaatg cattcgagaa
    56581 caagcgcccg cccggtgacg gcgcccgcga tatcgaggat gttgtcagcg ttctctttca
    56641 gttccgacac gagcggtgtt gtgtccgcta tggggacgta aataccggtc aatgccgttg
    56701 gtgcctgcgc tggaacaacc tgcttcgcta gtgtcacttt ctcagcttcc gctgcggtca
    56761 ctaattgact atttcctaca gccgctgact cacttctgag ttgatagtcg ttcattctca
    56821 tggttaaaaa catgaccttg gtgtggtctc cggcgcctgg atttagttga gcatatatcc
    56881 ccataaccac gttcttcccc ggccgacggc atagctgaag attacacgga ttaaggggct
    56941 gttctccaaa ttgccaccaa tgctcgtagc caaaggtact gctggctggt ccgtattttt
    57001 ttataagatc agacatcacg gcggttataa gtggttgttt catcgcgccc tcgaatatgg
    57061 cgccccgata tattccgtag accacgttgc cggtggttgg gttgctaaaa tatacggtga
    57121 cactatcgcg agtcccattt tgaaacgcga cctggctgat gaaagactgc gacttcgcga
    57181 aaattccatc atggttaacc gagaggaaag ttctgatttc tctcggcttg cccggatatg
    57241 tcttggcggc gatcgcctcg gcctgtgcaa ctgtcatgcc aatacggatg ccaaggatgt
    57301 cagccgtgac gccagggtag gcgtgggggg tgggcttgac cgtttgcacg agtgtgaccg
    57361 agacttgatg ctgctcagct ttggcgctgg gcatcagggc tgtcaacgcg ataaggccga
    57421 tcggaccgac gtgtgttgct ttcatggccc cctccgttat gtacggaaca gctaaaagta
    57481 catcccaccg agcgatctcg gttgaatgat acgatgcgcc gcgaacgcca tatatcatcc
    57541 aatcaaacac tcctctcacg caatcggcct aatccttcaa aaggctggac agaccaccac
    57601 gaaagacgca gcatgttcat tgggtggatc acgcccgcac cgaatgcgca gtactgcgtc
    57661 atgaccggga ggtgtccgtg tgaacaccgc caagtgccaa tacctggacg agggcatcat
    57721 gaagatcaag catgatatcg tcgcgcttgt tctagctggc gtggccggca tggcaaccgg
    57781 caccaacgct gcagcgcaat gcaccaccac acctttcccc ggccctccca aggaagtgct
    57841 gatacgctgt actctggcgc aaacggctca gccgatcgca ccgccgacat accccggggt
    57901 cgatttcaat atattcgggt ttcggacggg aatgacagtc gcccaggtcg aggccctcgc
    57961 cgcaaagatt catcttggta agcctggaat taaaaccaag cctctcgaca atgaggtgaa
    58021 aatgtgggga gcaacagcgt tggcccaagg ggctcccatc aatcaaatca gctacgactt
    58081 caggctggcg ggagggtcaa accgggcaac tgtgatcatc tacttcagca gccctgcaac
    58141 ggggagcgta agcgaagctg tatatgaaaa cagcaactac atcaccgata gaaggcagaa
    58201 gcggccgctg gtggcacccg tcgtgacaaa actcgtccac caatacgggc cctcttcgtc
    58261 ccgtacccca agctatatgt ctcaatacct ctgctggaac tttaaaggac aatcgctcgt
    58321 caaagcgtcc ctccgccaac cttgctatcc caatgctgac gacgtcatgg gcactaacgt
    58381 ggcggcgatg ggaaacggaa catatcaccc agcagattcc tatatttatg ctcttttatc
    58441 aaccaactcg ggggaggtca atggcttggc gcaagggctt ggggattacc gcctcgcggc
    58501 attggaccgc tctgaaataa agaagcaatt gacccttgca atcgaaaaat atagcccgcc
    58561 cactcagacg tcggcgagca cccaccgttg atgcgttggg gtcgtgcgtg aggcgtgcgg
    58621 gtgtccgcaa accgaccgca cccatgatcc acccgaccca cagagaaact cctggaattt
    58681 cagaagctct gctcgatgtg gttattggct attttccatt cagttgcacg gataaggcgg
    58741 ttctcgtcat atggaacgca gtggatgaac cctgcgcgga tgcgggtctc acaaacgtgt
    58801 tttttattac aaaacccgca aaccttcccg acgccacaac ccaagagtac gtccttccgg
    58861 ttttcatccc gtttgaaccc aaggaggttt cggatcctac tttgtaagcc tgaaaagttc
    58921 cggctggaac tgttaccgac tcgatcccca gcaatactcg tgtggtttgc gacgtgacac
    58981 ggtcctcgac accatcagtg gcaagggtgt gcatggtatc tgtttcggta tacggcgtat
    59041 tcataacaaa acagcgcgga agggatatcg gtggatcgtt atacgtctct ccccccgagc
    59101 gcacgcgcgc accatagaga tagacgaatt gcgcaccaac cccataaaag tgatcgatga
    59161 tcgatacgtt cttatgcgag gggtatgcaa gctcaatatt ctctcgtata gcgatcgcct
    59221 tttggccgcg gaatgtggtt ggccccaaga cttccgagtc gttttcttcc gtgtattcgc
    59281 gcccgccact ttcctcaatc ttggtaacga ctttgtaagt cgtccccttc ggatatgagg
    59341 ccggatagaa gggacaagtg tcaccggaga atttcaggtc ctggcttgta gcaactgtga
    59401 cgcccgtgct ggtcgaatta cccgaagaag aagaagaaga atgcatcggg gcgttgtacg
    59461 cactggcttg agcgtacgtc atcgacattc cccatactag aatgctcaga acagcggttc
    59521 ctgatttcat gttttcaccc tcctccaatg ccaagccatg tcaaggagag ccataatccg
    59581 ggcttggcaa tgagatcagc ccaccacgtc gacgggtgct tcaagttgat ttgctcacac
    59641 attgcctgac gcaacagtcg attcgattcg aatcgggttt gcgttgatcc gatgcccccg
    59701 ctttcaacca gaatcattgg tgggtttgca caaccttttc ccaatttctg aacgactgtg
    59761 atcccaccga tggttatatt gggcctttta ttattgtgcg accataggcc gttggtggtg
    59821 ggaggcggat tttggttcgc acggcggtta cacacgcaca ccgttccgct cggtcaacgg
    59881 cgcttttttc gctgatcgct cagcaagcgg ttggccagga tattcacatg ccgaaatcaa
    59941 ccatccgatt gctggcggct tttctgatcg ggccgtgcgc gccagcgtgc gccgccaacc
    60001 ctcccgttgc cacgatccaa ctgagcggga gcaaaacact gccctacttc atctcggccc
    60061 cacccggacc acctgggccc attcgcgcgc tgatcggcgt gcaggggttt ccgcgtgatg
    60121 ccaaccgcac ctttgacgcc atcgcacgtg ccactcgcga agccggcaag accgcaacga
    60181 cactgctggt cgcccccatc ttccccgt
//
LOCUS       DASTNK010000127        15394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1209036, whole genome shotgun sequence.
ACCESSION   DASTNK010000127 DASTNK010000000
VERSION     DASTNK010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15394
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209036"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..507)
                     /locus_tag="VFR20_09590"
     CDS             complement(295..507)
                     /locus_tag="VFR20_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230813.1"
                     /translation="MPLLGPSSAAVPATPDVLNARQQYFSAEQNLDTARYRCLLSRLN
                     LAGLVGTLKDQDEQVVDGYPSDQPRR"
     gene            743..1396
                     /locus_tag="VFR20_09595"
     CDS             743..1396
                     /locus_tag="VFR20_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230814.1"
                     /translation="MKVSKKLTHTPVALAAALGLLFGGATAASAATGTAPAPAHAMAM
                     NWFGGPVYNGEPALAATAALVKAGGGAEHFTFAHALVSMLGEKTVNAEVAKLTKQYGK
                     KDVDGFLNGMTFAIKDGLKRATEAGVKLPEAPADLKGVKLARALVQAGTAPDGTFWAG
                     YLFDKAISHKLHNQVMVDIDVKYGHGADKNTHKILNQAMHDVAQALGDTHVKLASLH"
     gene            1582..2448
                     /locus_tag="VFR20_09600"
     CDS             1582..2448
                     /locus_tag="VFR20_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEU0230815.1"
                     /translation="MKKILGVYSAPKGHWVGDGFPVRSLFDYNSHGKHLSPFLLLDYA
                     GPADFSPASQPRGVGQHPHRGFETVTIVYKGEVEHRDSTGAGGKIGPGDVQWMTAASG
                     ILHEEFHSREFTRTGGTLEMVQLWVNLPARDKMSGPGYQTLLDGDIPAVTLPDGAGKV
                     RVIAGDYEGHRGPAHTHTPMNVWDVRLDQGREARLRAVPGHTLVIVVLHGTVQVNGSQ
                     IARDAQMVVFDRDDGEVLLEANSNAVLLLLSGEPIDEPIVGYGPFVMNSAAEINQAFA
                     DFNSGRFGAMAL"
     gene            complement(2488..2649)
                     /locus_tag="VFR20_09605"
     CDS             complement(2488..2649)
                     /locus_tag="VFR20_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018026506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HEU0230816.1"
                     /translation="MLHYAVVFLVIALIAGFFGFFGIAGAAAGIAKILFAIFIILFVI
                     SLVFGRRPP"
     gene            complement(2662..2880)
                     /locus_tag="VFR20_09610"
     CDS             complement(2662..2880)
                     /locus_tag="VFR20_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230817.1"
                     /translation="MNSQYLKHHARKIHIDERHPVDTWITEITRRFRASGITLPTRLC
                     CESDQAFLSRVARRFPETAVGYPGLPEL"
     gene            complement(3578..5542)
                     /locus_tag="VFR20_09615"
     CDS             complement(3578..5542)
                     /locus_tag="VFR20_09615"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEU0230818.1"
                     /translation="MSQPPEVQSETRPGRTRGKTAEEQAENASTTRKLTTMLAVLGVV
                     YGDLGTSAIYSLQAVFNTHTGSGGLLAPNAPNVLGILSLVFWTLIVVVSLKYMTFILQ
                     ADNRGEGGTFALIALLRPWRNINRFTRHALVLIGLTGAAMLYAGVMITPAISILSAVE
                     GLEVASTDLADYIIPVTLVILVLLFAVQRFGTARIGAAFGPIMALWFVAIAALGVYGI
                     LKEPQVLGAIDPRYAARFFRDSGITGFLVLFAVFLVTTGAEALYADIGHFGRTPVRKV
                     WFFFVLPALLLNYFGQAAMLLHDPKAASQPFYHLIPTALLYPIVVLATLATIIASQAA
                     ITGAFSMTRQAARLGMMPRSQIVQTSSEVSGQIYVPVMNWILMAAAIILVVLFKSSDQ
                     LASTYGISVSSAMVVTTILAFFVARERGHWSLGAALLFLVGFLVVDLAYFGSNLLRIP
                     HGGWFPVIVGALFFTVMSTWRRGSELLARQSGKDAKPIDELIRDLENGEVARVPGTAV
                     FLTLWLKDTPPSLHHHIERNHALQEQVVLLTVLTEDVPHTTREERVEYENLGNGFHRV
                     VLHYGYMQGVNVPSELTTCEEKGLTIELDSVTYYVEHQSPISGSGRHDGMIAWRDHLL
                     GFLMRNSTDASTGYQIPSDKIVDMGLRVRI"
     gene            5790..6578
                     /gene="hutC"
                     /locus_tag="VFR20_09620"
     CDS             5790..6578
                     /gene="hutC"
                     /locus_tag="VFR20_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine utilization repressor"
                     /protein_id="HEU0230819.1"
                     /translation="MKTSSLSLNTAVSPGSSRASGAPALALYEQIKEFISRKIQDGSW
                     PAGHRLPSEHELVAQFGISRMTVNRALRELAGQGRVVRVAGVGSFVAESKPQSTLLKI
                     VSIEHEIRSRGHDYRFELLAVERASAAPDVAAWLDLRAGASVFHSVCLHFENGTPVQL
                     EDRYVNPQVVPGFLDHDLATMPPGEYLLRNVPFDEVEHVVDAVLPSAGQAARLAMDAT
                     APCLLLTRRTWTRGTAVTWVRCLHPASRYRLGSRFRADGNPHPG"
     gene            6665..8239
                     /gene="hutH"
                     /locus_tag="VFR20_09625"
     CDS             6665..8239
                     /gene="hutH"
                     /locus_tag="VFR20_09625"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEU0230820.1"
                     /translation="MNDRYDFAGTLVDTLTLMPGAVELATLRRIHGGQVRLDLDDSAR
                     AGILAAQATVRDIVDKGEVVYGINTGFGKLAQTIIPPERLAELQRKLVLSHSVGTGEP
                     LPDGVVRLVLATKAVSLARGHSGARSEIIDALLALFNAGLLPRIPSKGSVGASGDLAP
                     LAHLACVLIGEGEVATPEGDTIDGRAALARVGLEPFVLGPKEGLALLNGTQVSTALAL
                     AGLFGVEQVFAAGLMAGALSLEAVQASVKPLDARIHAARGQLGQIAVAGALRTLLDGS
                     QIVPSHAHCGHVQDPYSIRCVPQVMGAVLDNLAHAARVLTIEANAASDNPLVFSENGD
                     VISGGNFHAEPVAFAADILALAVSEIGAISERRLALLLDTNLSGLPPFLVRDGGVNSG
                     FMITQVTAAALASENKSLAHPASVDSLPTSANQEDHVSMATFAARRLADMVNNTAVVT
                     GIEAMAACQGIELRRGPLSSPLLETEFATIRAHVAFIEEDRFMAPDVETMRRWALGHD
                     GREWPAPLTAILPSHG"
     gene            8329..10053
                     /gene="hutU"
                     /locus_tag="VFR20_09630"
     CDS             8329..10053
                     /gene="hutU"
                     /locus_tag="VFR20_09630"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEU0230821.1"
                     /translation="MNAPDIHVSLATGADPRHDPTRVIHAPRGSQLNCKSWLTEAPFR
                     MIQNNLDPDVAERPQDLVVYGGIGRAARDWACFDQILATLKTLEDDETLLVQSGKPVG
                     VFRTHPDAPRVLIANSNLVPRWATWEHFNELDRKGLFMYGQMTAGSWIYIGSQGIVQG
                     TFETFVEAGRQHYGGDWAGRWILTAGLGGMGGAQPLAATLAGAASLNIECQQSSIDFR
                     LRTHYVDKQARDIDDALALVKHHTERGEAVSIALLGNAAETLPELVKRAQAGGIRPDL
                     VTDQTSAHDLINGYLPIGWSVEQWRDAQRDAAQQPHLKAEAAASCAKHVRAMLDFQKM
                     GVPVVDYGNNIRQVAFDEGVDNAFDFPGFVPAYIRPLFCEGKGPFRWVALSGDPEDIY
                     KTDAKIKELFPDNTHTHRWLDMARERIAFQGLPARICWLGLGERHKAGLAFNDMVRTG
                     ELKAPIVIGRDHLDTGSVASPNRETESMRDGTDAVSDWPLLNALLNTAGGATWVSLHH
                     GGGVGMGYSQHAGVVIVCDGTDAAAKRIERVLFNDPATGVMRHADAGYESAVATARQH
                     GLNLPMIG"
     gene            10075..10674
                     /locus_tag="VFR20_09635"
     CDS             10075..10674
                     /locus_tag="VFR20_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HEU0230822.1"
                     /translation="MSVHRFRADELPAVPWKNGGGTTRQVACWPPGAGFDDFEWRVSV
                     ADIATSGPFSAFPGVDRHILLLSGDGMRLRAGDGGFDHRLAVPLVPFAFSGDVAVQSD
                     MLGGVSSDFNVMARRGRCRALVRVIDDVRELDASAHGLLMVAAGAWRANGEALGVGSG
                     LWWAREPLAWRLVPTESRAKIIAVSFSEVEENRRRQRSE"
     gene            10799..12073
                     /gene="hutI"
                     /locus_tag="VFR20_09640"
     CDS             10799..12073
                     /gene="hutI"
                     /locus_tag="VFR20_09640"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEU0230823.1"
                     /translation="MTPPFESLPSADGVWMNVRLWAPEAQEGNEARVGGKAPAVPEAA
                     SGTMVVQAGHIAWLGSHSSLPTTYQTLPRHDGGGALAMPGLVDCHTHLVYGGQRADEF
                     AMRLAGADYEAIARTGGGIVSTVRATRDADEDVLYAQAAGRLEQLLDEGVCAIEIKSG
                     YGLALEHERKQLRVARRLAAAYGVTVRTTFLGAHALPPEFAGRSADYIALVCEEMLPA
                     LAEEGLVDAVDVFCERIAFSLDETERVFRAAKALGLPVKLHAEQISDMGGAVLAARHG
                     ALSCDHLEHLSKDGVAAMRDAGTVAVLLPGAYYTLRDTHLPPVAALRAAGVPMAVATD
                     HNPGTSPALSLLLMANMACTLFRLTVPEALAGITTHAARALGLQETHGALAVGRPANF
                     VLWRVEDAAELAYWFGHRPSCTIVRQGRIAKG"
     gene            12316..13116
                     /gene="dsbG"
                     /locus_tag="VFR20_09645"
     CDS             12316..13116
                     /gene="dsbG"
                     /locus_tag="VFR20_09645"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HEU0230824.1"
                     /translation="MLSRPSLYRLFLGALALFLSMTGYAAETALPPSVKALQSQGLDV
                     MGEFNVGDGLRAFAGAAGDRPLAIYVTQDGSAIIGTRVGPDGKALDEGRLQDLVAKPM
                     AKRAWSALEKATWVQDGKKDAPRIIYTFSDPNCPYCHKFWEASRPWVKSGAVQLRHLV
                     VAVIRPDSATKAATILGAKDRSAELTKNEMTFSEGGIKPAESVPPAIQTILDENQELM
                     MNMGFRGTPGIVAMRSDGLLEKINGLPQPAALAELLGPNPGSPSAKTK"
     gene            complement(13261..13788)
                     /locus_tag="VFR20_09650"
     CDS             complement(13261..13788)
                     /locus_tag="VFR20_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese family protein"
                     /protein_id="HEU0230825.1"
                     /translation="MNLESIQPRAAQALLADGAILIDIRTADEYAREHIADARHLPLQ
                     QLQNGAMPPKDATALIFHCRSGARTRANADLLARSTQCPAYMLEGGLDAWKKTGLPVR
                     ADASQPLELQRQVQIAAGSLVLLGAVLGAAISPWFHLLSAFVGLGLVFAGVSGFCGMA
                     RLLMRMPWNRRMHSA"
     gene            complement(13809..14120)
                     /locus_tag="VFR20_09655"
     CDS             complement(13809..14120)
                     /locus_tag="VFR20_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU0230826.1"
                     /translation="MVSDDQSKLFRKEGAAKAAAMLRTVGNEHRLLVLCLLIEHQEMT
                     VGALLEHVDLSQSALSQHLAKMRDEGLVTFRRESQTLHYRIANPDVATLITALKAIFC
                     P"
     gene            14299..15186
                     /locus_tag="VFR20_09660"
     CDS             14299..15186
                     /locus_tag="VFR20_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0230827.1"
                     /translation="MTSPIDIQAFFDDASGTVTYLVSDPATRQAAVIDPVLDYAHRNG
                     KVATRSADQVLAFAQDAGLVIVRILETHAHADHLSAAPYLRRHTSAKVAIGEHIREVQ
                     VIFRPVFNLDDVSGDGSEFDELLADGATLALGDATIEVMHTPGHTPACVSYRIGDAVF
                     VGDTLFMPDYGTARADFPGGSARNLYRSIRRLLDLPPDTRLFVCHDYKAPGRDVYAWE
                     TTVAAQRAGNVHIHDGVDEDAFVAMRERRDAGLAAPELLLPAIQVNIRAGRLPLAESN
                     GARYLKIPITLDANAFDIE"
     gene            complement(15252..>15394)
                     /locus_tag="VFR20_09665"
     CDS             complement(15252..>15394)
                     /locus_tag="VFR20_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEU0230828.1"
                     /translation="QHLAGMKDSKVIVAINKDAEAPIFGVADYGLVADLFQAVPELTA
                     AL"
BASE COUNT         2922 a         4510 c         4954 g         3008 t
ORIGIN      
        1 catgacggaa ccgcgctgtg agtcgacccc ggtcagagca atgggtcgga ggaatcgcag
       61 tgcggtggtt gccggcgcgg ttcgctggct gcatctcgca gcctcgccga cgtttgtcgc
      121 catggcgctc ctgggagggg gtttcggtgg cggtatgcca aacgcgcctt gtccggccat
      181 gcctgtgtcc gcatggacgg ggatgggcct gatgtacttg ctgatgagcg tcttccacgc
      241 gggcccgtgg ctcaagctgg tttctttttc acgaaatcag gaaatacggc atgactaacg
      301 ccgtggttgg tccgatggat agccgtcgac aacctgctcg tcctggtcct tgagcgttcc
      361 caccaatccc gcgagattca gccgggacag caggcaccga tagcgtgccg tgtccaggtt
      421 ctgttcggcc gagaaatatt gttgccgcgc attcaacaca tccggcgttg caggcaccgc
      481 tgcggatgac ggccccaaca gtggcacatg gatcttgaca gtttcattga tcggtatata
      541 gtagctatac aaacaactac taatggccaa ttcaggttca ccgacctgat caggcccagc
      601 gccatggata cgatcaagac gcccaagcag ttcggattca ctgtgtactc atgtgggaag
      661 ggggttccca tccacttgct gtcgttgccc cagagccttc tctagctccg gagccatatt
      721 ctttaccttg aaggaattca tcatgaaggt ctcgaagaaa ctcacccata ctccggtagc
      781 gctggccgcc gccctcggtt tgctgtttgg cggcgccaca gccgcgtcgg ccgccaccgg
      841 cacggcaccc gctccagcgc atgccatggc catgaactgg ttcggcggcc cggtctacaa
      901 tggcgagccc gccctggcgg ccaccgctgc actagtcaag gctggcgggg gggccgagca
      961 cttcacgttc gcacatgccc tggtttccat gctgggcgag aaaaccgtga acgctgaagt
     1021 cgccaagctg accaagcaat acggcaagaa agacgtggat ggcttcctga atggcatgac
     1081 ttttgccatc aaagacggct tgaagcgtgc caccgaagcc ggcgtgaagc tgccggaagc
     1141 ccctgccgac ctcaagggcg tgaaactggc ccgcgcgctg gtgcaggccg gaaccgctcc
     1201 cgatggaaca ttctgggccg gctatctgtt cgacaaggcc atctcgcaca agctgcataa
     1261 ccaagtcatg gtcgacatcg acgtgaagta tggccacggt gcggacaaaa acacgcacaa
     1321 gatactgaat caggcgatgc acgacgtggc gcaggcactg ggcgacacgc acgtcaagct
     1381 tgcatcactc cactgacgcg gcgttcgtga gaggcccgcg tggttcaacc cgcgcgggct
     1441 tctcacccgg ccatcgttcc ataaatagaa cgaaaagtat aaaaccagcc atctattcgc
     1501 gttgtcgtat cggatttaac atcctcttca ctaggtctta agcaaaacac ccgctttgcg
     1561 ttcttacgga gaacacctca agtgaagaag attcttggcg tctacagtgc ccccaaaggt
     1621 cattgggttg gtgacggttt tccagtccgc tcgctgttcg actacaacag ccatggcaaa
     1681 cacttgagtc cgttcctgct gctggactat gccgggccgg ccgatttctc gccagccagc
     1741 cagccgcggg gcgttggcca gcacccgcat cgcggatttg aaaccgtgac catcgtctac
     1801 aaaggcgagg tcgagcatcg cgattccacg ggggcgggcg gcaagatcgg tccgggagat
     1861 gtccagtgga tgacggccgc gtcgggcatt ctgcacgagg agtttcattc ccgtgagttc
     1921 acacgtaccg gtggcacgtt ggaaatggtg cagttgtggg tgaatctacc ggctcgcgac
     1981 aaaatgtccg ggccgggata ccagactctc ctggacgggg acattcccgc tgtcaccttg
     2041 ccggatgggg ccggcaaggt gcgtgtcatc gcaggggact atgagggcca tcgtgggccg
     2101 gcacacacgc acaccccgat gaacgtctgg gatgttcggt tggatcaggg gcgcgaggcg
     2161 cgtctgcgcg ctgtgccagg tcatacgctg gtgatcgtgg tgctgcatgg aaccgtgcag
     2221 gtcaatggtt cgcagattgc ccgcgatgcc cagatggtcg tgttcgatcg cgacgacggg
     2281 gaggtgttgc tggaggcgaa cagcaatgct gtcttgttgc tgctgagtgg cgagccgatc
     2341 gacgagccta ttgtgggcta cgggccgttt gtgatgaaca gcgcggcgga aatcaaccag
     2401 gccttcgcgg atttcaacag cggccgtttt ggggccatgg ccctttgacc gccatggccc
     2461 tttgaccgtc ggaggtgacg acatgattca gggaggtctg cgcccgaata ccagcgatat
     2521 gacaaataga atgatgaaaa tggcgaacag aatcttggct attcccgcag cggcgcccgc
     2581 gatgccaaaa aagccaaaga acccggcgat cagagcgata accagaaaga cgacggcata
     2641 gtgcagcata cattctcctt gtcagagctc aggcagaccg gggtagccca ccgcggtttc
     2701 agggaatcgg cgagcgactc ggcttagaaa cgcctggtcg ctttcacagc aaaggcgagt
     2761 cggcaacgtg atgcccgacg ctctgaagcg acgggtgatt tcggtaatcc aggtatccac
     2821 ggggtggcgt tcgtcgatat ggattttcct ggcatggtgc ttcaagtatt ggctattcat
     2881 ctgattctcc tatttgaagc cgtcgcgacg cactcaagtt gtcgtggcga actcgagttg
     2941 tcacgacgta ttcaagttgc cacaactcat tcaaggcttg acgatcaaat tgttatgcat
     3001 ggccgtgaca cccttgactg agcgggcgag ttggccggcg cggtcttttt cactctgcgc
     3061 ttgcgtcgat gggtgtcgtg cggctttttt tgcatgctgc cacggacatt aagaccaagc
     3121 gacaccgcaa taaccgattg cgcgactggc catctgcttg gggttcgtcg tcttcatgcg
     3181 cggtacccgt tgtaccgccc ctgcactctc gtggctggtt gaatcaacgg tatcatggcc
     3241 cttgcggccg tcggtactac ggcttgtaac tatattgtcg aaacgggttg ggatctgtca
     3301 gggaaaggct gacgatattc agccgtcgcg gcggaccgaa aaaccacgtg gcgcctgcga
     3361 tgtcggcgct cgcgggccat ggcgcaggtt gtcactcaaa acgtggtttc attgtgacac
     3421 gacttttttg cccgccgtct tgaggcttgt cgattcagcc actgtattgt gctccgatga
     3481 gcggccaccg cgagggtggc aaccgcgcgg aatggcccga aagaagtggc tatcgcacga
     3541 cgcatgacaa ctgtggcttg aggcatgaca acctgaatca aattcgcacg cgtagcccca
     3601 tgtccacgat cttgtcgctg gggatctggt agccggtact ggcgtctgtg gaattgcgca
     3661 tcaggaaacc cagcaggtga tcgcgccagg caatcatgcc gtcgtgccgg ccgctgccac
     3721 tgatgggtga ttggtgttcc acgtagtacg tcacgctgtc gagttcgatt gtcaggcctt
     3781 tttcttcaca tgtggtgagt tcggaaggca cattgacacc ctgcatgtag ccatagtgca
     3841 ggacgacacg atggaagcca ttcccaaggt tctcgtattc cacgcgctcc tctcgcgtgg
     3901 tgtgtggcac atcttcggtc agaacagtaa gcaagaccac ctgttcttgc aaggcatgat
     3961 tgcgttcgat atggtgatgg agtgaaggcg gcgtgtcctt gagccagagt gtcaggaaga
     4021 ccgcggtgcc cggcacgcgt gccacctctc cgttctcgag atcacgtatc agttcgtcga
     4081 ttggtttggc gtccttgccc gattgacgag ccagcagttc gctaccacgg cgccaggtgg
     4141 acatgactgt gaagaacagt gcgccgacta ttaccgggaa ccaaccgccg tgtggaatcc
     4201 gcagcagatt ggaaccgaag tacgcgagat ccacgaccag gaatccaacc aggaacagca
     4261 gcgcggcccc caacgaccag tgtcctcgct cgcgcgccac aaaaaaggca agaatggtgg
     4321 tgacgaccat tgcggagctg acactgatgc cgtaggtgct ggccaattgg tccgagctct
     4381 tgaacaacac caccaggatg atcgccgccg ccatcaggat ccagttcatc accggcacat
     4441 agatttgccc ggatacttcg ctggaagtct ggacgatttg cgagcgtggc atcatgccga
     4501 gccgagcggc ctggcgggtc atggaaaaag ccccggtgat cgcagcctgc gaggcgatga
     4561 tggtcgccag ggtggccagc accacgatcg ggtagagcag agcggtcgga ataagatggt
     4621 agaaaggttg tgaggcggcc ttggggtcgt gcaacagcat ggcggcctgt ccgaaataat
     4681 tgagcagcaa tgctggcagc acgaaaaaaa accacacttt tcggactggc gtgcgaccga
     4741 aatggccgat atcggcatac aacgcttcgg ctccggtggt caccagaaac acggcgaaca
     4801 gcaccaggaa gccggtgatg ccgctgtcgc gaaaaaaacg ggccgcatac cgcggatcga
     4861 tcgcgcccag cacctggggt tccttcagga tcccgtagac gcccagcgcg gcgatggcca
     4921 cgaaccacag ggccatgatt ggtccaaacg cggcgccgat ccgggcggtt ccgaaacgct
     4981 gcacggcgaa cagcagcacc aggatcacca gggtgacggg aatgatgtaa tcagccaggt
     5041 cagtggatgc gacctccagg ccttccaccg cgctcaagat ggagatggcg ggggtgatca
     5101 tgaccccggc gtacagcatc gccgccccgg tgaggccgat gagcaccaat gcgtgccgtg
     5161 tgaaacgatt gatgttgcgc caggggcgca acagcgcgat cagtgcgaat gtgccgccct
     5221 cgccacggtt gtcggcttgc aatatgaaag tcatgtactt caatgacacc acgacgatca
     5281 acgtccagaa cacgagcgac agaatgccca gcacattcgg ggcgttgggg gcgagcaacc
     5341 cgccgcttcc cgtgtgggta ttgaataccg cctgcaacga gtagatggcg ctggtgccca
     5401 ggtcgccgta gaccactccg agcaccgcga gcatggttgt cagtttccgg gtggtgcttg
     5461 cgttttcagc ttgctcctcg gcggtcttgc cgcgtgtgcg gcctgggcgc gtttcgctct
     5521 ggacttcagg cggctgactc atggccggcc ccactgaaac gaccgtctgg gcgtcgctgg
     5581 cattgtcatt tgacgtttcg ctcggttgtt tggctatccc gagtataggc ttgtgcccat
     5641 tttgcctggg ttccgcgggt cttagggaaa ataagtatat acaggtatag atattacggt
     5701 cagaataaca agcgtactta acgaatggct tcgtgtgctg gctatctctg ggcattaatt
     5761 gggttccgaa acattcatca ggccaagcca tgaaaacatc ctcgctttcg ctcaacacgg
     5821 ctgtctcgcc cggctcctcc agggcatccg gtgcgccagc cttggcgctg tacgagcaaa
     5881 tcaaggaatt catctcgcgc aaaatccagg acggctcatg gcccgccggc cacaggctgc
     5941 cgtccgagca cgagctggtc gcccaatttg gcatttcccg catgacggtc aaccgcgcgc
     6001 tgcgcgaact ggcgggccag ggccgcgttg tgcgggtcgc gggcgtgggc agtttcgtgg
     6061 ccgaaagcaa gccacagtca acgctgctca agatcgtcag tatcgagcac gagattcgca
     6121 gtcgggggca cgactaccgc tttgaattgc tggcggtcga gcgcgcgtcg gccgcaccag
     6181 acgtcgccgc ctggctcgat ctgcgtgcgg gggcgtcggt gtttcactcg gtctgcctgc
     6241 atttcgagaa tggcaccccg gtgcaactgg aagaccgtta cgtcaacccc caggtcgtgc
     6301 ccggcttcct cgaccatgat ctggccacca tgccgccggg cgagtacctg ttgcgcaacg
     6361 tcccgttcga cgaagtcgaa cacgtggtcg atgcggtgct gccttcggcg ggccaggccg
     6421 cccggctggc aatggatgcc acagctcctt gtctgttgtt gacgcgccgc acctggacgc
     6481 gcggcacggc ggttacctgg gtacgctgtc tgcacccggc gtcgcgctac cgcttgggca
     6541 gccggtttcg cgccgacggc aatccgcatc ccggttgacg gtcgccactc aggaaagcga
     6601 tcatcgtcgt tttttggcgg attcctgtat atacaagatt agatagctag aggatgaaag
     6661 agcaatgaac gataggtacg attttgccgg caccctcgtc gatacgttga cattgatgcc
     6721 gggcgccgtt gaattggcga cactgcgtcg catccacggc gggcaagtgc ggctcgatct
     6781 cgacgattca gcacgcgcgg gcattttggc cgcgcaggca accgtgcgcg acatcgtcga
     6841 taaaggtgaa gtggtctacg gtatcaatac cggcttcggg aagctggcgc aaaccatcat
     6901 cccccccgaa cgcctcgccg aactacagcg caagctggtg ctgtcgcaca gcgtggggac
     6961 cggcgagccg ttgcccgatg gcgtggttcg gcttgttctg gccaccaagg cggtgagtct
     7021 ggcgcgtggt cactcggggg cgaggagcga aatcatcgat gcgttgctgg ctcttttcaa
     7081 tgccgggctg ctgccgcgca ttccgagcaa ggggtccgtt ggcgcgtcgg gtgacttggc
     7141 gccgctggcg catctggcct gcgtgctgat aggcgaaggc gaagtggcca cgccagaagg
     7201 cgacacgata gacggccggg ccgcgctggc gcgtgtgggg cttgagccct tcgtgcttgg
     7261 ccccaaggaa gggttggcgt tgctcaatgg cacgcaggtg tcgaccgcat tggcgctggc
     7321 gggcctgttt ggcgtggagc aggtgttcgc ggccggtctg atggcgggcg cgctgtcgct
     7381 ggaggcggtt caagcatcag tcaagccgct ggacgcgcgc atccatgcag cgcgtggcca
     7441 gttgggccag atcgcggtgg ccggtgcgct gcgcacattg ctggatggca gccagatcgt
     7501 gccgtcgcac gcgcattgcg gtcatgtgca ggacccgtac tcgatccgct gcgtgccgca
     7561 agtgatgggc gcggtgctcg acaacctggc gcacgcggcg cgcgtgctga ccatcgaggc
     7621 caatgcggcg tccgacaacc cactcgtgtt cagtgaaaac ggcgacgtga tctctggcgg
     7681 caacttccat gccgagccgg tagcgtttgc cgccgacatc ctggctttgg ccgtgagcga
     7741 aatcggtgcg atttccgagc gccgattggc gttgctgctg gataccaacc tgtccggcct
     7801 gccaccgttc ctggtgcgcg atggtggcgt gaactccggt ttcatgatca cgcaggtcac
     7861 ggcggccgcg ctggcgtcag agaacaaatc actcgcccat ccggccagcg tggacagtct
     7921 gcccacgtcg gccaaccagg aagaccacgt ctcgatggcc accttcgccg cgcggcgcct
     7981 ggccgacatg gtgaacaaca cagcggtggt gacgggcatc gaggccatgg cggcatgcca
     8041 gggtatcgag ctgcggcgtg ggcccctttc ctcgccgctg ctcgagacag agttcgccac
     8101 gatccgcgcg cacgtcgcct tcattgaaga ggaccggttc atggcgcccg atgtcgagac
     8161 catgcgccgg tgggcgctgg ggcacgatgg tcgggaatgg ccggcgccgc tcaccgccat
     8221 tctgccgagc cacggatgaa cgctgacgtc tccctagtgg gtatgacgca tgactggctt
     8281 gaatcaccgt cttcgagcca gtatgaaact tgttgacagg acactatcat gaacgcaccc
     8341 gatatccacg tctctctcgc caccggcgcc gatccgcgcc atgatccgac acgcgtgatc
     8401 cacgcgccgc gtggcagtca gctcaactgc aaaagctggc tgaccgaggc gccattccgc
     8461 atgatccaga acaacctcga cccggatgtg gcggaacggc cgcaagactt ggtggtttat
     8521 ggcggcattg ggcgcgccgc gcgtgactgg gcctgtttcg accagatact cgccacgctc
     8581 aagacgctcg aagatgacga aacgctgctg gtgcagtcgg gcaaaccggt cggtgtgttc
     8641 cgcacgcacc cggatgcgcc acgggtgctg atcgccaatt cgaacctcgt cccgcgctgg
     8701 gccacttggg aacatttcaa cgagctcgac cgcaagggct tgttcatgta tggccagatg
     8761 accgccggaa gctggatcta catcggcagc cagggcatcg tccaggggac gttcgagaca
     8821 ttcgtcgaag cgggccgtca gcactatggc ggcgactggg cggggcgctg gattctcacc
     8881 gccggcctag gtggcatggg cggtgcgcag ccactcgccg ccacgctggc cggcgcggct
     8941 tcgctcaaca tagaatgcca gcagtcgagc atcgacttcc gcctgcgtac gcactacgtt
     9001 gacaagcagg cccgcgatat cgatgacgcg ctggcactgg tcaagcatca cactgaacgg
     9061 ggggaggcgg tgtctatcgc cttgctgggc aacgcggcgg agaccctgcc ggagctcgtt
     9121 aagcgtgccc aggcgggtgg tatccgcccc gatctggtga ccgaccagac ttcggcgcat
     9181 gacctgatca acgggtacct gcccatcggc tggagcgttg agcaatggcg tgacgcgcag
     9241 cgcgatgccg cccagcagcc gcacctgaag gccgaggccg cggcttcatg cgcgaagcat
     9301 gttcgtgcca tgctggactt tcagaagatg ggtgtgccgg tggtcgatta tggcaacaac
     9361 atccggcagg tggcgttcga cgaaggcgtc gacaacgcgt tcgatttccc ggggttcgtt
     9421 cccgcgtaca tccgtccctt gttctgcgaa gggaaggggc ctttccggtg ggtggcgctg
     9481 tcgggcgacc ccgaggatat ctacaagacc gacgcgaaga tcaaggaact gtttcccgac
     9541 aacacgcata cgcatcgctg gctggacatg gcgcgtgagc gcatcgcgtt ccagggtctg
     9601 cccgcccgca tttgctggct gggcctgggc gagcgccaca aggcgggcct ggccttcaac
     9661 gacatggtgc ggactgggga attgaaggcg cccattgtca tcggacgcga ccatctcgac
     9721 acggggtcgg ttgccagccc caaccgagaa acggaaagca tgagggatgg caccgacgcg
     9781 gtgtccgact ggccgctgct caacgcactg ctgaatacgg ctgggggtgc cacctgggtc
     9841 agtctgcatc atgggggcgg cgtcggcatg ggctattcac agcacgcggg tgtcgtgatc
     9901 gtgtgtgatg gcacggatgc cgcggcgaaa cgcatcgaac gcgtgctgtt caacgacccg
     9961 gccaccggcg tcatgcgcca tgccgatgcc ggctacgaat ccgccgtggc gaccgcgcgc
    10021 cagcacggcc tgaacctgcc gatgatcggt tgatccgccc gggataacga gaacatgtca
    10081 gtgcatagat ttcgagcgga tgagttaccc gccgttccct ggaagaacgg cggcggcacc
    10141 accaggcagg tggcttgctg gcctccaggg gcgggtttcg acgatttcga atggcgtgta
    10201 agcgtggcgg acatcgccac gagcgggcct ttctccgcgt tcccgggcgt ggaccggcac
    10261 attctgctgt tgtcaggtga cgggatgcgg ctacgcgcgg gcgatggtgg gttcgatcat
    10321 cggctggccg tgccgctggt gccgttcgcg ttctccggcg acgtggctgt gcagtctgac
    10381 atgctgggcg gcgtatcgag cgacttcaat gtcatggccc ggcgtggccg ctgtcgcgcg
    10441 ctggtgcgcg tgatcgatga cgttcgcgaa ctcgacgctt ccgcgcacgg tctgctcatg
    10501 gtcgcggcgg gagcgtggcg ggccaacggt gaagcgctcg gtgtcgggtc ggggctgtgg
    10561 tgggcgcggg agccgcttgc ctggcggctt gttcccactg aatcaagggc gaagatcatt
    10621 gccgtgagct tctccgaagt tgaggaaaat cgtcggaggc aacgaagcga gtgagcgagg
    10681 aaaagacaat gggccgcacg agtgagaaac aggcagtgaa ccgcatgagc gagaaggcgg
    10741 ctggggatag cgccaaaagg aagacttcag gggacggcgc gatccggaag gagccttcat
    10801 gacgccgcca tttgaaagtc tcccgagcgc cgacggtgtc tggatgaacg ttcgcctgtg
    10861 ggcgccggaa gcacaagaag gaaatgaggc tagggtaggg gggaaagcgc cggcagtgcc
    10921 agaagccgcc agcggcacaa tggtggttca agccgggcat atcgcctggt tgggatccca
    10981 ctcctcgctg cccaccacgt atcagacgct gccacgccat gacggcggcg gcgcgctggc
    11041 catgccgggc ctcgtcgatt gccacaccca tctggtctat ggtggccagc gcgccgacga
    11101 gttcgcgatg cggctggccg gcgccgacta cgaggccatt gcccgcaccg gggggggcat
    11161 tgtctcgacc gtccgcgcca cgcgtgacgc ggatgaggac gtgctgtatg cgcaagccgc
    11221 tggacgattg gagcaactgc tggacgaagg ggtgtgcgcc atcgagatca agtcgggcta
    11281 cgggctggcg ctcgaacacg aacgcaagca gttgcgtgtg gcgcgccgac tcgctgccgc
    11341 gtacggagtc acagtgcgca cgacgtttct tggcgcgcac gcgctgccgc cggaattcgc
    11401 agggcgtagc gccgattaca tcgcgctggt ctgcgaggaa atgcttcccg ctttggctga
    11461 ggaaggcctg gtcgatgcgg tcgacgtttt ctgcgagcgc atcgcgttct ccctggacga
    11521 aacagaacgt gtgttccggg cggcgaaggc gcttggcctg ccggtcaagc tgcacgcgga
    11581 acagatctct gacatgggcg gcgcggtgct ggcggcgcgc cacggcgcgt tgtcttgcga
    11641 ccatctcgaa catctgtcga aggatggtgt tgccgccatg cgggacgcgg gtacggtggc
    11701 tgtgttgctt ccgggagcgt attacacctt gcgcgacacg catctaccac cagtggcggc
    11761 attgcgcgcc gcaggggtgc cgatggcggt cgccaccgac cacaatcccg gcacgtcccc
    11821 ggcgctgagc ctgctgttga tggccaatat ggcctgcaca ctgtttagac tgacggtgcc
    11881 ggaggctctg gccggcatca ccacgcatgc ggcgcgcgca ctcgggttgc aggagacgca
    11941 cggcgcgctt gccgtagggc gcccggcgaa tttcgtgctc tggcgggtgg aggacgctgc
    12001 cgagctggcc tattggttcg gtcataggcc gtcttgcacc attgtgcgcc agggccgtat
    12061 cgcgaaagga tgagggggcg cggcgaccgc gtcatgcctt cctgaagaaa ttcacagcgt
    12121 cgggttccag gccgaatcat ccatcctgat gaatcgccga cgtccgttgc cagcggcgct
    12181 ggcgtgatgg gtgaggccac catgccggcc tgaaaagcac tataaagagg tatcgcgggg
    12241 ggttcaccgc tatcttttca aggagtcccc ggaacaaggt gatttgttcc ggggactcca
    12301 agctcaggaa tcatcatgtt gtcacgtccc agtctatatc gtttgttcct cggggcgttg
    12361 gccctgttct tgtcgatgac gggttacgcg gctgaaacgg cactcccccc gtccgtcaag
    12421 gcgcttcagt cgcaagggct ggatgtcatg ggtgagttca atgtgggcga cggtttgcgc
    12481 gcgtttgccg gtgccgccgg agatcgtccg ctggcgatct acgtcaccca agacggcagc
    12541 gccatcatag gcacgcgtgt ggggccagat ggcaaggcgc tcgacgaagg ccgtctgcaa
    12601 gacttggttg ccaagccgat ggccaagcgc gcctggtcgg cgctggaaaa agcaacctgg
    12661 gtgcaggatg gcaagaaaga cgcgccgcgc atcatttaca cgttcagcga ccccaattgt
    12721 ccgtactgcc acaagttctg ggaagcctcc cgcccctggg tgaaatccgg cgccgtgcaa
    12781 ctgcgccacc tggtcgtcgc ggtgattcgc cctgacagcg ccacgaaggc cgcgaccatt
    12841 cttggcgcca aagaccgctc cgccgagctc acaaaaaacg aaatgacatt ttccgagggc
    12901 ggcatcaaac cggcggaatc ggtgccgccc gccattcaga ccatcctgga tgagaaccag
    12961 gaactgatga tgaatatggg gtttcgcggc acaccgggta tcgtcgcgat gcgcagcgac
    13021 ggtttgctgg aaaaaatcaa cggcctgccg cagccggccg cgctcgcaga gctgttgggg
    13081 ccgaaccctg gttcgccttc cgccaagaca aaataacccc atgctggccg ctgtggccgc
    13141 gtgcatgagt cagccaattt cagggacgga ttgagccggc gtgctgaggt ccgatgcgtg
    13201 ctgcatacga accaagcgtg gtcgccgggg tggcctggct ggatgtcgtg ggagcgccgg
    13261 ctacgccgaa tgcattcgac gattccatgg catcctcatc aacaggcgtg ccatgccgca
    13321 aaagccggat acgccggcga acaccagccc cagtcccacg aacgctgaaa gtaggtggaa
    13381 ccacggtgag atcgccgctc ccagcaccgc gccaagcagg acgagcgagc ccgccgcgat
    13441 ttgaacctgg cgctggagct ccagcggttg tgatgcgtcg gcccgcaccg gcagcccggt
    13501 tttcttccat gcgtccagcc cgccttccag catgtaggcg gggcactggg tgctgcgcgc
    13561 cagcaggtcg gcattggccc gggtgcgggc gcccgagcgg caatggaaga tgagggccgt
    13621 tgcgtctttc ggcggcatgg cgccattctg cagctgttgc agcggcaggt ggcgggcgtc
    13681 agcgatgtgt tcgcgggcgt attcgtcggc ggtgcggata tcgatgagta tcgcaccgtc
    13741 cgccagcagc gcttgtgccg cgcggggctg tatggattcc agattcatga tggtttctct
    13801 tgcaagtgtc atgggcagaa gatggctttg agagcggtga tgagcgtggc cacatcagga
    13861 ttggcgatgc gatagtgcaa tgtttgagat tcgcgccgaa aagtgaccag tccttcgtcg
    13921 cgcatctttg ccagatgttg cgacagggcg gactgactga gatcgacgtg ttccagcagc
    13981 gctcccaccg tcatttcctg atgttcgatc agcaggcaca gcaccagcag ccggtgttcg
    14041 ttgccgacgg tgcgcaacat ggccgccgcc ttggccgcgc cttccttgcg aaacaacttg
    14101 ctctggtcat ccgataccat atgacgttga taaaaatagc gagtggtggg aggagatctg
    14161 taatctgaag gttctcgaat tccctgatga tcgagtcgga attgagtctg gatcgggttc
    14221 aagaggtttt aatttagaat atcgtaaatt agcatttact aattaataga tcaacctaat
    14281 ttaaagaaag gaaacgccat gacttcgccg atcgacattc aggcgttctt cgatgacgct
    14341 tccggcacag tgacctatct ggtctccgat ccggccaccc gtcaggcggc cgtgatcgat
    14401 cccgttctgg actatgcgca tcgcaatggc aaggtggcca cccgctcggc tgatcaggtg
    14461 cttgcgttcg cccaggatgc cggtttggtc atagtccgga ttctggaaac ccacgcgcac
    14521 gccgatcacc tcagcgcagc gccctatttg cggcggcaca ccagcgcgaa agtcgccatc
    14581 ggcgagcata tccgggaagt gcaggtcatt ttccgcccgg tgttcaacct ggatgatgtc
    14641 tcgggtgatg gcagcgagtt cgacgaattg ttggcggacg gcgccacgtt ggcgttgggt
    14701 gacgcgacga tcgaggtgat gcatacgccg ggacacacac cggcgtgtgt gtcgtaccgc
    14761 atcggtgatg cggtattcgt gggggatacc ttgttcatgc ccgactatgg cacggcgcgc
    14821 gctgactttc cgggcggcag tgcgcggaac ctgtatcgct cgatccggcg gttgctcgac
    14881 ctcccgcccg atacccgctt gttcgtctgt cacgactaca aggcgcctgg gcgcgatgtt
    14941 tatgcctggg aaaccacagt ggcggcacaa cgtgcgggta atgtccatat ccacgatggt
    15001 gtcgatgagg acgcgtttgt cgcgatgcgt gaacggcgcg acgccggcct ggccgcgccc
    15061 gagctgctgc tgccggcaat ccaggtgaat atccgtgcgg ggcgccttcc gctcgctgag
    15121 tccaacggtg cgcggtattt gaaaatcccc ataacgctcg acgctaacgc tttcgatatt
    15181 gaatagtcgt attaacggag tggaatgtat agtaacgtgc tggtgtacgc atcatcagca
    15241 cgtcccggcc atcacagagc ggcggtcagt tcgggcacgg cctggaacag gtcggccacc
    15301 aggccgtagt cggccacccc gaagatgggc gcctcggcgt ccttgttgat ggccacgatg
    15361 accttggagt ccttcatgcc cgccaggtgc tgga
//
LOCUS       DASTNK010000128         2963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_654693, whole genome shotgun sequence.
ACCESSION   DASTNK010000128 DASTNK010000000
VERSION     DASTNK010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654693"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1194)
                     /locus_tag="VFR20_09670"
     CDS             complement(1..1194)
                     /locus_tag="VFR20_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEU0230829.1"
                     /translation="MSSTPSFNWQDPLLLDTQLNEEERMVRDAAQNYAQDKLAPRVLE
                     AFRHEKTDASIFPEMGELGLLGPTIPVEYGGAGLNYVSYGLIAREVERIDSGYRSMMS
                     VQSSLVMVPINEFGSDAQKQKYLPKLARGEWIGCFGLTEPNHGSDPGSMETRAVKTAD
                     GYSLTGNKMWITNSPIADVFVVWAKCVGGEHDGKIRGFILEKGAKGLSAPAIHGKVGL
                     RASITGEVVMDEVAVPEDQMLPGVSGLRGPFTCLNSARYGIAWGALGAAESCWHTARQ
                     YTLDRKQFGRPLAANQLIQKKLADMQTEITLALQGCLRLGRMKDEGTAAVEITSIMKR
                     NSCGKALDVARMARDMLGGNGISDEFGVARHLVNLEVVNTYEGTHDVHALILGRAQTG
                     LQAFF"
     gene            complement(1319..>2963)
                     /locus_tag="VFR20_09675"
     CDS             complement(1319..>2963)
                     /locus_tag="VFR20_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU0230830.1"
                     /translation="QLVAQGVEHVFCVPGESYLAVLDGLHDADIQVTVCRQEGGATMM
                     AEAHGKLTGQPGICMVTRGPGASNALAGIHIAHQDSTPLIVFVGQIDRGTRGRDAFQE
                     MDYKAVFGTQTKWAVEIDQIERIPELVSRAFHVATSGRPGPVVVALPEDMLAERAVVS
                     DAPRHQTLDAWPAPAQLADLSQRLSQAKRPVAILGGARWNAQAVSDFADFAQRHALPV
                     AVEFRRQMLFPTTHPNFVGDVGLGGNPALMAALREADLFLLVGGRMSEVASQGYTLLN
                     IPVPAQQLVHVYPDAAELNRVYHASLAIQASPIAFTGALRDMRAGAAGGTAERLQALR
                     ASYQAWSDPSRISHPGTLQMGGIMRYLNDTLPDDAILTNGAGNYATWLHRFYAFRRYG
                     TQLAPTSGSMGYGLPAAVGAKRLHPDRTVVCFAGDGCFMMHGQEFATAVQYGLPIIVI
                     LVDNGMYGTIRMHQERHYPGRLSATTLHNPDFVAYAQAFGGHGERVDTTEQFAPAFER
                     ALASGKPAILHCRLDPEAITPTTTLTKIREQAMAAAASR"
BASE COUNT          499 a          923 c          993 g          548 t
ORIGIN      
        1 ttaaaaaaac gcctgcaatc cagtctgggc acgccccaga atcagggcat gcacgtcgtg
       61 cgtgccttca taggtgttga ccacttccag gttcaccagg tggcgcgcca cgccgaattc
      121 atcggaaatg ccgttgccgc ccagcatgtc gcgcgccatg cgcgcgacat ccagggcctt
      181 gccgcaggaa ttgcgcttca tgatggacgt gatttccacc gcggccgtgc cttcgtcctt
      241 catgcggccc aggcgcaggc agccttgcag cgccagcgtg atctcggtct gcatgtcggc
      301 cagctttttc tggatgagct ggttggcggc cagggggcgg ccgaactgtt tgcgatccag
      361 cgtgtactgg cgggcggtgt gccagcagga ctcggcggcg cccagggcac cccaggcgat
      421 gccgtagcgg gccgaattca agcaggtgaa cggaccacgc aggcctgaca cgcctggcag
      481 catctggtcc tccggcacgg cgacttcatc catgacgact tcgccggtga tggaggcgcg
      541 cagccccact ttgccgtgga tggcgggggc cgacagaccc ttggcgccct tctccaggat
      601 gaagccgcgg atcttgccgt cgtgttcgcc gcccacgcac ttggcccaca ccacgaacac
      661 gtcggcgatg ggcgagttcg tgatccacat cttgttgccc gtcagactat agccgtcggc
      721 ggtcttgacg gcgcgtgttt ccatgctgcc ggggtcggag ccatggttgg gctcggtcag
      781 gccgaagcag ccgatccatt cgccgcgcgc gagtttgggt aggtattttt gtttctgcgc
      841 gtcgctgccg aattcgttga tgggcaccat gaccagggat gattgcacgc tcatcatcga
      901 gcggtagccg gaatcgatgc gttcgacttc ccgggcgatg aggccataac tgacgtagtt
      961 cagccccgcg ccgccgtatt cgacgggaat ggtgggcccc aacaggccca gttcgcccat
     1021 ttcggggaag atggaggcgt cggttttctc atggcggaac gcttcgagca cgcgcggcgc
     1081 gagcttgtcc tgcgcatagt tctgcgcggc atcgcgcacc atgcgctctt cttcgttcag
     1141 ttgggtgtcc agcaacaggg gatcctgcca gttgaaggag ggggtggaag acatgggatg
     1201 gctccggctg agatgagatg ggtcaggggt gaacccacgc gcttgttggt tcacccatat
     1261 agtttaaacc taaaatatat gcgaatccgt tcgcgacgat atccgtatcc tgtgcatgtc
     1321 agcgtgatgc ggctgctgcc atcgcctgct cgcggatctt cgtcagtgtc gtggtggggg
     1381 tgatggcctc cgggtcgagc cggcagtgca ggatggcggg cttgccgctg gccagtgccc
     1441 gttcgaaggc gggcgcgaat tgctccgtgg tgtcgacccg ctcgccatgg ccgccgaacg
     1501 cttgcgcata ggcgacgaaa tccggattgt gcagcgtcgt ggcggatagc cggccggggt
     1561 aatggcgttc ctgatgcatg cgtatggtgc catacatgcc gttgtcgacc aggatgacga
     1621 tgatgggcaa tccatactgc accgctgtgg cgaactcctg gccgtgcatc atgaagcagc
     1681 catcaccggc gaagcacacc acggttctat cgggatgcag ccgcttggcc cccaccgccg
     1741 cgggcagccc ataacccatc gaacccgacg tgggcgccaa ttgtgtgcca tagcgccgga
     1801 aggcgtagaa gcggtgcagc caggtggcat agttgccggc gccgttggtc aggatggcgt
     1861 catccggcag cgtgtcgttg aggtaacgca tgatgccacc catctgcagc gtcccgggat
     1921 gcgaaatccg gctcggatcg ctccaggcct gatagctggc gcgcagcgcc tggagccgtt
     1981 cagcggtgcc gccggccgcg ccggcccgca tgtcgcggag cgcgccggtg aaggcgatgg
     2041 gtgatgcctg gatggcgagg ctggcgtggt agacacggtt gagttcggcc gcgtccgggt
     2101 acacatgcac caattgctgc gccggcacgg gaatgttcag caaggtatag ccctggctgg
     2161 cgacttccga catgcgtccg cccaccagca ggaacaggtc agcctcgcgc agggccgcca
     2221 tgagcgccgg gttgccgccc aggcccacat cgccgacgaa attgggatgc gtcgtgggaa
     2281 acagcatctg gcgccggaat tcgaccgcca ccggcagggc atggcgctgc gcgaaatcgg
     2341 cgaaatccga caccgcctgt gcgttccagc gcgcgccgcc cagaatggcc accggcctct
     2401 tcgcctggct cagccgctgc gacagatcgg ccagttgcgc cggcgcgggc cacgcgtcca
     2461 gcgtctggtg gcggggggcg tcggacacca ccgcgcgttc ggccagcata tcttccggca
     2521 aggcgacgac caccgggcca gggcgccccg aggtggcgac atggaaagcc cgcgacacca
     2581 gctccgggat gcgctcgatc tggtcgatct cgaccgccca tttggtctgt gtgccgaaca
     2641 cggccttgta atccatttcc tggaaggcat cgcgtccgcg cgtgccgcga tcgatctggc
     2701 ccacgaagac gatgagtggg gtggaatcct gatgcgcgat gtggatgccg gccaatgcgt
     2761 tggacgcacc cgggccgcgg gtgaccatgc agatacccgg ctggccggta agcttcccgt
     2821 gggcctcggc catcatggtg gcgccgcctt cctggcgaca cacagtgacc tggatgtcgg
     2881 catcatgcag gccgtccagc acggccaggt agctttcgcc gggaacacag aatacgtgct
     2941 ccacgccctg ggcgacgagt tga
//
LOCUS       DASTNK010000129        20219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1580128, whole genome shotgun sequence.
ACCESSION   DASTNK010000129 DASTNK010000000
VERSION     DASTNK010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 20219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20219
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580128"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VFR20_09680"
     CDS             <1..633
                     /locus_tag="VFR20_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0230831.1"
                     /translation="IWWALCLNVTAGIGVIGQASVMIQESFKGAVTPAAAAGFVGLLS
                     LANMLGRFLWSYASDYLGRKNTYYIFLALGAVLYLLVPSAGAAGYVAVFVLFYTIIIS
                     MYGGGFSTLPAYLADMFGTRFVGGIHGRALTAWAAAGIFGPVLVNYIRQYQVNHGVPP
                     SQAYTETMYIMAGLLVVGIICNFFVRPVSSHHHMTAEQLGTATAQPAMGH"
     gene            653..772
                     /locus_tag="VFR20_09685"
     CDS             653..772
                     /locus_tag="VFR20_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate:formate antiporter"
                     /protein_id="HEU0230832.1"
                     /translation="MEERTTTSPALLIIFWLYVGIPLVLGIWETLIKASALFK"
     gene            complement(844..1305)
                     /locus_tag="VFR20_09690"
     CDS             complement(844..1305)
                     /locus_tag="VFR20_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU0230833.1"
                     /translation="MFKRILIPTDGSPLSAQAANKGVCFARQIGAEIVALYVTQPFAA
                     TVGFDGMAAAYAITDEDYEKTAAEQAQKYLKAVLDRASTAGVKAVGKAVSNFNVADGL
                     VQATEDEKCDLIFIGSHGRSGLSRLLLGSVTIKVLSLAKVAVLVYRVKESD"
     gene            complement(1406..1747)
                     /locus_tag="VFR20_09695"
     CDS             complement(1406..1747)
                     /locus_tag="VFR20_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HEU0230834.1"
                     /translation="MSVQISVLTPDFAVSPQIAVEDVPDIAAAGFKSIIINRPDFEGG
                     PDQPPSAEVIAAATAAGVRAEYLPVISGAMTREDVVQFAALLKDLPAPVLAYCRSGTR
                     STNLYQAAIGL"
     gene            complement(1856..2692)
                     /locus_tag="VFR20_09700"
     CDS             complement(1856..2692)
                     /locus_tag="VFR20_09700"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEU0230835.1"
                     /translation="MKTMGDIKHIVICADDFGMHPGIDTAVLQLAGQGRLGATSCLVD
                     GASFARNAKALSVSGLQIGLHLNFTERLETTPDPAALSLPVSTLIARSYLRQLDEVRV
                     RRQIVRQLDRFESVLGRAPDFVDGHQHVHQLPQIRQCLMAELVHRYPHKRLWVRNTRM
                     GRLGMTGSFRFKAGVIQGLGAASLVRLARGAGFAVNQGFLGVYDFQGGREAYGRLLKT
                     WLGAAGSASVLMCHPASQSAGDVLGDQRVAEFEVLADKRTAAWLEEYGLSVGGLSVDA
                     IP"
     gene            2726..3115
                     /locus_tag="VFR20_09705"
     CDS             2726..3115
                     /locus_tag="VFR20_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU0230836.1"
                     /translation="MRKILSQLSWFVAVGCAAAATHWLVVVAVVSLASLAPLAANVIG
                     WLVAFGVSFSGHYQLTFRHLRAPVLRAARRFFLVSALGFCVNEASYAWLLRATTIRYD
                     VLLAIILIAIAGLTFILGRFWAFRSKI"
     gene            complement(3075..4658)
                     /locus_tag="VFR20_09710"
     CDS             complement(3075..4658)
                     /locus_tag="VFR20_09710"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU0230837.1"
                     /translation="MNRLVQLTSRMLEIRSTWLVAIITFLWVSATTWTRPLMLPDEGR
                     YVGVAWGMLDVGQAWVPRLDGLPFFHKPPLFYWVTELSLWLFGVNEWAARLCSVLAAT
                     LMAAMFFWLIKTFADRRVAILATLVLVTAPFFVGGGQYANMDMTVAAAITATVVLGAA
                     AVFRMERGERYRLVLTLAYAAAGFGFLAKGLIGIALPGGIIVFWLAGRRRFDLMRRMF
                     IWPGFVALAVVALPWMVYMEWHFPGFFHYYIIYQQFDRFLETSFNNRQPFWFYVPVLL
                     VMTLPWSTQVWRLARGAYWKNAELGAFRGLMVSWFLVVLIFFSIPASKLIGYILPLLP
                     PFAFFLAELFAQRLGGDSSQRALKVLSRYWVVSACLCLIAVIVLVVSPRRTTKYLAEQ
                     VRTQYQPADKVVMLDYYYYDFNFYLRTAKSAMIVSRWKDPEILLHDGWRKELYDAGQF
                     DPAAADKLLITSQTFREKLCGPRAVNLWLVGDTDSLGDFPFLKTLPVRFKSKKLRAWF
                     VPAGEPLKFCSGTPKTAPK"
     gene            complement(4938..5915)
                     /locus_tag="VFR20_09715"
     CDS             complement(4938..5915)
                     /locus_tag="VFR20_09715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0230838.1"
                     /translation="MSCVVPSLNEEANLRVLLPALIAVLRTLAPRYEIIVVDDGSTDA
                     TSGLFTSWADTHPEIVYLRLSRNFGKEAALSAGIEAAHGQVVVCLDADLQHPPALIPQ
                     MLDRWRHGVDMVYAVRETRADESLFKRVGTKLFYRLMRSSNGLQVPENAGDFRLMDRR
                     VVDALRMLPERDRFMKGLFAWVGFGSEPFYYTPPGRLHGTTTFKPFKLVRFAFDGLTA
                     FTTWPLRMFSLAGMGLSLLSFAYGLYVVVNHWIYGDVVQGWATIIAVILFFAGVNLMS
                     LGVVGEYVARIFSEVKGRPVYLIKDRIGTGLGVPADANGLSGRDRDQAA"
     gene            6161..6583
                     /locus_tag="VFR20_09720"
     CDS             6161..6583
                     /locus_tag="VFR20_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HEU0230839.1"
                     /translation="MPEAALCTEEEVTRLVHGFYGRVRADSQLGPIFDAHVGDWDTHL
                     AKMVQFWSSLLRGTGTYRGAPMPAHIALPGLNAGLFRQWLALFHDTTREIPNRPFAER
                     AEEFAHRVARSLWFGYQISREPDRAPLELTDDEYPQHA"
     gene            6558..7775
                     /locus_tag="VFR20_09725"
     CDS             6558..7775
                     /locus_tag="VFR20_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEU0230840.1"
                     /translation="MTSIPSTLDNSMPTTTARPQWRAFLSLGFRPLYFAGCAWAAVAI
                     AIWIFAPGLLKQPLGGLAWHAHEMLWGFVATIAVGFLLTASANWTKTNPLHGWPLAVL
                     VSLWLVARIGYLAGGRAAFLLAGAAETAFFVVAGVAVLRVVIMAGSRRNYGIPLLLFG
                     LATANVLYLRAAQQGDYAELMRRFDLGLLCMAVIALLIARRVIPFFTMRMVQGLSIPM
                     NTRSGQAQITLGLVAILTGMVGFYLVMAAALAATGLIGLYQIVAWRPLASRHRPILWI
                     LYLGYAMLSLGLVSAAIYLVNPVPGVLARAATHVHIIGVGGFSILIIGMVTRTALGHL
                     GRPLKLDASMRVSYLTLIIATALRLAALPATGPANLWLLRLAAAFWIATFVLYLWRFA
                     PMLIRPRPDAARS"
     gene            7788..8255
                     /locus_tag="VFR20_09730"
     CDS             7788..8255
                     /locus_tag="VFR20_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU0230841.1"
                     /translation="MRLTSMTDYAIRLLMYVACHPGRLCTIGEIAQAYHISEPHLMKI
                     TNRLSQCGWIETVRGRNGGMRLARTPTDIRLGAVMRDTETDFELVECFGPGSTCTLTG
                     QCRLTGIIDGALRAFLRHMDDYTLADILPAASYPIFLDTHNATVSQARPKNPA"
     gene            complement(8189..9346)
                     /locus_tag="VFR20_09735"
     CDS             complement(8189..9346)
                     /locus_tag="VFR20_09735"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEU0230842.1"
                     /translation="MKKWILGLGASLLVASRAFALSIPAPALDANAWLLLDAKSNEII
                     AEHNADARIQPASLTKIMTAYLVFKAIHDHQLSMDQTVTVSTLAWKVDPESSKMFLKP
                     GDRVTIRQLLTGLLVDSGNDAAVALAEATAGSVGTFVAEMNQMAAHMGLKSTHFASPH
                     GLPSPETYSTARDLSELARHLIQDYPKLYVGFDTIKQYTYDKITQQNRNKLLWIDPSV
                     DGLKTGHTAEAGYCLIASAHRPGATVDRRLISVVLGTPSSKARVQESETLLNWGFGNF
                     DTVKLYGKQQVVATPRVWKGEQDQVKVGFTHDVYLTVGRGAKLLGVPKVTTKGPLVAP
                     IAADAQVASLTETVGDRTVTLPLVALHPVGQAGFFGRAWDTVALWVSRKMG"
     gene            complement(9343..11178)
                     /locus_tag="VFR20_09740"
     CDS             complement(9343..11178)
                     /locus_tag="VFR20_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU0230843.1"
                     /translation="MRSPKVLSPSTKTTIPKLGDFTTDRRVLYLMGLAVVVGILGDLS
                     SWVLFKLLYLCTNVAYYGQWSFVNKGIDGSPLGWYMVFVPVVGSIVVGLMARYGSDKI
                     RGHGIPEAIEAILIGKSRIQPKVAILKPLSSAISIGTGGPFGAEGPIIMTGGALGSLL
                     AQTLNMSSSERKTLLVAGAAAGMTAVFGTPVASILLAVELLLFEWKPRSFLPVAIAAI
                     VAQALRPVFMPGAAPIFPYVGGVTLSTWHVAAWALMGVVAGFGSGLLTAIVYACEDLF
                     EKLPLHWMWWPALGGVVVGVGGLIEPAALGVGYDNIRHLLAGDVALKAAFLLLVVKVI
                     IWSVALGSGTSGGVLAPLLIIGGTIGAVLAPWFPHADPGFWALVGMAAMMGGTMRAPL
                     TSTLFAVELTGNYSVLLPVLTACLFAYGLTVLLLKRSILTEKVARHGHHLTREYHVDA
                     FDLERAGSVMTKSVKTLPGTMTIAEAIEFFTTTEFRHSSYPVVDDGQRVLGLVTRADS
                     LAWTVDRENGNDRGATTLQEILRDQELLIGYPDEMAGRIADRMAVTGVGRIPIVDRDT
                     DQLLGLIARKDLLRVRVHRLHEEGDRKAYLRWRLAKRRRVKKVSV"
     gene            complement(11466..11909)
                     /locus_tag="VFR20_09745"
     CDS             complement(11466..11909)
                     /locus_tag="VFR20_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0230844.1"
                     /translation="MKLTDRPAHTNDAGQLTPADYQALATFRQALRKFIAFSETEAAT
                     AGLMPQQHQALLAIKAMSTSDAPSVGELADYLMIRPHSAVELVGRLVRMGLVERLRDP
                     NDRRRVRVSLSRLAESKLENLSSAHLKELGAIRPLMGRLFGRFEH"
     gene            complement(12011..13210)
                     /gene="serA"
                     /locus_tag="VFR20_09750"
     CDS             complement(12011..13210)
                     /gene="serA"
                     /locus_tag="VFR20_09750"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEU0230845.1"
                     /translation="MTRIVLFENIHASAREVFEAAGFDDIKTYASSLPPADLPDALRG
                     AALVGIRSRTHLTAELLAGLPDLRAVGCFCIGTNQVDLDFAMAHGTPVFNAPYSNTRS
                     VAELVLGEAILLLRRIPEKNARVHQGHWDKTAEGAYEARGKTLGIIGYGNIGSQVGTL
                     AESAGMRVIFHDIEAKLPLGNARACAYLNDLLAQSDVVTLHVPGGKSTRNIMSAAAIA
                     VMKRGAILINASRGTVVDIDALRGALENGSLSGAALDVFPAEPKSVDEPLASPLIGMH
                     NVILTPHIGGSTQESQENIGREVAEKLVRFAQGGATKGAVNFPEIPYVESSGAARILH
                     VHRNVPGALGALSSLLAEHGLNIVTQQLQTRGQIGYVISDVDGSVTDEVMDALRRHPI
                     TIRCDRM"
     gene            complement(13312..14154)
                     /locus_tag="VFR20_09755"
     CDS             complement(13312..14154)
                     /locus_tag="VFR20_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEU0230846.1"
                     /translation="MRTFEFKQVDVFSDTPLKGNPLAVVVEADGLADEQMAAFANWTN
                     LSETIFLLKPTRPEADYRVRIFTPQAELPFAGHPTLGACHVWLATGGRPRGERIVQEC
                     AVGLIPLRRDGRRVAFAAPPMVRTGPVDAPLLRRIADGLGISHDDVLDAQWVDNGPGW
                     LAVLLGSRRQVLNLKPDFVRLNGIKLGVVAPWDEAVDGSDAQFEVRAFVPDLGVPEDP
                     VTGSLNASVAQWLIGTDRAPASYVAAQGTALGRAGRVHVHSDGPDIWIGGDVSTCIEG
                     TVTL"
     gene            complement(14226..14948)
                     /locus_tag="VFR20_09760"
     CDS             complement(14226..14948)
                     /locus_tag="VFR20_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0230847.1"
                     /translation="MNEPTFLPASLEPQAVDRRSLPASIANQLRDMIIEGLLPAGARL
                     NERALCDQLGVSRTPLREALRQLSAQGLVWIQPNRGARVVALSVDDVRESFQVMGALE
                     ALSGELACQHVTDTEIAEIRALTFEMRACHARQDLPSYYRLNCAIHDHINRAARNHLL
                     SQVYGTLNLRIQNLRFRSNLNHDKWDAAMHEHIQMVEALAAHDGPRLAGILRVHMQRK
                     GEAVLEVLAAQAGEADTALHEA"
     gene            15259..16458
                     /locus_tag="VFR20_09765"
     CDS             15259..16458
                     /locus_tag="VFR20_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0230848.1"
                     /translation="MIPLDTHPSGRHFLQIPGPTNVPDRIRRAISQVTIDHRGPEFGT
                     LGKAVLAGMRKIFQTDGPVVIFPASGTGAWEAALVNTLSPGDRVLMAETGQFATLWKN
                     LAARLGLEVEFLPGDWRHPVDSSQVEARLAADTQHDIKAVCIVHNETATGVTSDIAAV
                     RQALDRATHPALFMVDTISSLAAIDYRHDEWKVDVTVAGSQKGLMLPPGLSFNALGAK
                     ALAASGTAALPRSYWDWKPMLDLNPSGYFPYTPATNLLYGLAEAVDMLLDEGLDNVFA
                     RHKRLGEATRRAVRAWGLEVLCADETAHSAALTAVLMPPGHSADAFRKTVLERFDMSL
                     GQGLGRLKDRVFRIGHLGDINDLTLCGVLAGIEMGLSAAGVPHDKGGTQAAIDFLAAT
                     SATPREA"
     gene            16463..18328
                     /locus_tag="VFR20_09770"
     CDS             16463..18328
                     /locus_tag="VFR20_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEU0230849.1"
                     /translation="MSDSDSESSAWHVPPSLYARQREVAFTLPGRPASVYVPMRDGCR
                     LALDYYLPQTDRGGPRAEDRFPVIALFTPYYRRFALADASLGTEPTPNAGKYRDYFVP
                     RGYALVVVDVRGTGASFGTRDSFRSPRERDDYREIADWIVAQPWCNGVIGATGISYLG
                     AACDFLASTGHPAVKAIAPISAVWDTYSDHYYPGGLFLNRLSQTYDRLMVALDHDRRD
                     LLGEFGYFSDPNYAGPQPVDDDTDGRACRAAVHGHLGNFRMPDFITGFACRDDALPYD
                     PAFTSASISPYHYRDGIRPDVAVLSVSGWMDGAGYANGSIARFLTLNGNPGYLLLGPW
                     DHGARVNTSPWRKSVTPQCNPFDEILRFFDHYLCGMDTGLRREEPVHYFSVHEEAWHA
                     AAEWPPLRGSATLSLASGQRLAGPAEADEANTGADGFDDVNVDFSFGTGNGTRYDRIA
                     GHDSRDYHTDWKQRQETLFSYTGDPLPADAELTGHAIADLWMASSEPDAAIHLYLSEV
                     LENGEVHYVTEGLLRALHRHEAPAPAAYRATWPFRTFSRADASPMPVDTPQNLRFALL
                     PVSWLFRRGSRIRLSIAGADADHVAQAPHGRPPRLRLWRGARQPSSLALPLRFKP"
     gene            18351..19895
                     /locus_tag="VFR20_09775"
     CDS             18351..19895
                     /locus_tag="VFR20_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0230850.1"
                     /translation="MKRLTQLALAGVAVLALQTPAMAGKSDNTIVWSTASEVDTADVY
                     YQNLREVVIASLNMCDSLVHLDPFTHKYEPLLATSYKWVDPHTLDFTLRKGIKFWNGK
                     EFGAKDVAYTLNHARQPDSGVVTRLAVDWIKNVEVVGPYEVRIIAKDPTPAALTYLAD
                     ETPIYPDGHYDSAPAIPGAKGQTRKDYGAVKPVCTGPYILKDFVPGSSFTLVKNTHYF
                     KGGPKGQPKVDKLVFRTIPDTDSQMASLMTHKVDWLWSVPPQNAKQLGAMPGITVKSA
                     PTTRLSFLSLDAAGRSGKNPMQNLKVRQAIAHAIDRKAIAHDLVGPGAQVLKSMCYPA
                     QFGCTEDVPQYNYDPALAKKLLAEAGYPNGFDQVIYAYRDRPYTEAVMNYLRAVGIRT
                     KLRFLQWKALRPLIDKGQVAMAQLTWGSQGMMDASASVSNYFEFSPDDYARDKQVRDW
                     LVAADHTTDPEARKTLYKKALTRIADQAYFIPLFTYARIYAFDSSLDYPVTPDELAHF
                     YLAHWK"
     gene            19966..>20219
                     /locus_tag="VFR20_09780"
     CDS             19966..>20219
                     /locus_tag="VFR20_09780"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0230851.1"
                     /translation="MLGFILKRLALSVLVVAVVSVVAFSLVYLSGDPAAAMAGEGATQ
                     ADVAAVRATYGFDRPLYAQYGSWLRNVMHGDFGRSYYLREP"
BASE COUNT         3940 a         6586 c         6040 g         3653 t
ORIGIN      
        1 atctggtggg cgctgtgcct gaacgtcacg gcgggcatcg gcgtcatcgg ccaggcctcg
       61 gtcatgatcc aggagagctt caagggcgcc gtcacgcctg ctgccgcggc cggcttcgtg
      121 ggcctgctct ccctggccaa catgctggga cgattcctgt ggtcgtatgc gtcggattac
      181 ctgggccgca agaatactta ttacatcttc ctggctcttg gcgccgtgct gtacttgctg
      241 gtgcccagcg cgggggcggc gggatacgtg gccgtctttg tgctgttcta caccatcatc
      301 atttcgatgt acggcggtgg gttctcgaca ctgccagcct acctggccga catgttcggc
      361 acgcggttcg tgggagggat acatggccgc gccctgacgg cctgggcggc cgctggcatc
      421 ttcggtccgg ttctggtgaa ttacatccgc caataccagg tcaaccatgg cgttcccccg
      481 tcgcaggcct ataccgagac catgtacatc atggccggcc ttctggtcgt gggcatcatc
      541 tgcaacttct tcgtgcgccc ggtatcgtcg caccaccaca tgaccgccga gcaattgggc
      601 acagccaccg cccagcccgc catgggccat tgaatctcaa ggagaccatc acatggaaga
      661 gcgcaccacc acctcacccg cactactgat cattttctgg ctgtacgtcg gtattccgct
      721 ggtgctggga atctgggaaa ccttgatcaa ggcgtccgcc ttgttcaaat aaacgcaaga
      781 cccgcacagc aaaacaccct ggaaccgcac agcggctcca gggtgtttta tctcaacaga
      841 tgttcagtcg gactctttga cccgatagac cagaaccgcc accttggcaa ggctcagcac
      901 cttgatggtg acactgccca gaagcaggcg cgacaagccg ctacgcccgt ggctgccaat
      961 gaagatgagg tcgcattttt catcttcggt cgcctgcacc aggccatcgg ccacattgaa
     1021 attggacacg gccttgccca ctgccttgac cccggctgtg gaagcgcgat cgagcacggc
     1081 cttgagatac ttctgggcct gctcggctgc ggttttttcg tagtcttcgt cggtgatggc
     1141 gtaagcggcc gccatgccgt cgaacccgac cgttgccgcg aaaggctggg tgacgtacag
     1201 cgccacgatc tcggcaccga tctggcgggc gaaacaaacg cctttgttgg cggcctgcgc
     1261 ggacaggggg gaaccgtcag tggggatgag tattctcttg aacatgatcg gcctcgcaag
     1321 tgatacgtgc ttccacaagg ttaaccagaa gtcgtgaaaa aaggaagccg gtcaatccct
     1381 aaccccgccg ccgaacacgc tctgactata gaccaatcgc cgcctgatac agattggtcg
     1441 accgtgtgcc ggaacggcaa tacgccagca ccggcgccgg aaggtctttg agcaacgcgg
     1501 cgaactggac gacgtcttca cgtgtcatgg cgccactgat caccggaagg tactccgccc
     1561 ggacgccggc cgccgtcgcc gccgcgatga cttcggcgga cgggggttga tcaggcccac
     1621 cctcgaaatc gggacggttg atgatgatgc tcttgaaacc cgccgccgcg atgtcgggga
     1681 cgtcttccac ggcgatctgg ggggacaccg cgaaatcggg ggtcagtacg ctgatctgga
     1741 cagacatgat gcgctccgca atgatgagaa ttcagttcga taacataaca tcattgtcgc
     1801 gatgtccaac gtttggaatc gacgacattt gagtccggcg acacttggaa gcagctcagg
     1861 gtatggcatc caccgacaaa ccgcccaccg acaaaccgta ttcctccagc caggccgccg
     1921 tgcgcttgtc ggcgagcact tcgaattcag cgacgcgctg gtcacccagc acatcaccgg
     1981 ctgactgact ggctggatgg cacatcagca cactggccga tccggccgcg cccaaccagg
     2041 ttttgagcaa ccggccataa gcctcgcgcc cgccctggaa atcgtacaca ccgaggaatc
     2101 cttgattcac ggcgaaccct gcgccgcggg ccaggcggac cagactggcc gcccccaacc
     2161 cctggatgac gccagccttg aaccggaacg aacccgtcat gccgaggcgc cccatgcgcg
     2221 tattgcgcac ccacaagcgc ttgtgcggat accggtgcac gagctccgcc atcaggcatt
     2281 ggcgtatctg gggcagttga tgcacgtgct gatgaccatc gacgaaatcg ggtgcgcgcc
     2341 ccaacaccga ttcgaagcga tcgagttggc gcacgatctg ccgccgcacc cggacttcgt
     2401 cgagctgccg cagatagctg cgtgcgatga gcgtcgatac cggcaggctc agagccgccg
     2461 gatcgggggt ggtttcgagg cgctcggtaa aattcaggtg cagaccgatc tgcagcccgg
     2521 atacactaag cgcttttgcg ttgcgcgcga atgaggctcc gtcgaccagg caactggtcg
     2581 cccccaaccg cccctgcccg gcaagctgca ggacagcggt gtcgatcccg gggtgcatgc
     2641 cgaagtcgtc ggcgcaaatg acaatgtgct tgatgtcgcc catggtcttc atgcaattcc
     2701 tccggcatcc cgataacagc gagtcatgag aaagatatta agccaactga gctggttcgt
     2761 ggccgtgggc tgcgccgctg ccgcaaccca ttggctggtg gtggtggctg tggtaagcct
     2821 ggcatcgctg gcgccgctgg cagccaatgt gattggctgg ctggtggcat tcggcgtgtc
     2881 gttctcgggc cattaccaac tgacgttccg gcacctgcgc gcccccgtgc tgagggcggc
     2941 aaggcggttc tttctggtat cggcgctggg tttctgcgtg aacgaagcca gctacgcctg
     3001 gctcctgcgc gcaacgacga tacgctacga cgtgctgctc gccatcattt taatcgcgat
     3061 tgccggcctg acgttcattt tggggcggtt ttgggcgttc cggagcaaaa tttaagtggc
     3121 tcaccggcgg gtacgaacca tgccctgagt tttttgctct tgaacctcac cggcaaggtt
     3181 ttcaagaacg ggaaatcgcc aagactgtcc gtgtccccga ccagccaaag attcaccgcg
     3241 cgtggaccgc acagcttttc ccggaacgtt tgcgacgtga taagcagttt gtcggccgcg
     3301 gccgggtcga attggcccgc atcgtacagt tccttgcgcc agccgtcatg caacaggatt
     3361 tctggatcct tccacctgga aacgatcatc gccgacttcg ccgtgcgcag atagaagttg
     3421 aagtcgtagt aatagtagtc gagcatcact actttatcgg ccggctgata ctgcgtgcgc
     3481 acctgctcgg ccagatattt ggtcgtgcgt cgtggcgaca ccaccagcac aatgacggcg
     3541 atcaagcaca ggcacgctga aaccacccag tatctcgaca acaccttcaa ggcgcgttgg
     3601 ctgctgtcgc cccccagccg ctgggcgaag agctcggcca ggaaaaaagc gaatggcggc
     3661 agcaacggca gaatgtagcc gatcagtttc gaagcgggga tcgagaaaaa gatcagcacg
     3721 accaggaacc aggacaccat cagtccgcga aacgcgccca gctccgcatt cttccagtag
     3781 gcgccccgcg ccagtcgcca cacttgcgtg gaccacggaa gcgtcatcac gagcaacact
     3841 ggcacataga accagaacgg ctggcggttg ttgaagctcg tctccaggaa acggtcgaac
     3901 tgctggtaga tgatgtagta atggaaaaag cccgggaaat gccattccat gtacaccatc
     3961 cacggtagcg ccacgacagc caaggcgacg aagcccggcc agatgaacat gcggcgcatc
     4021 aggtcgaagc gccgccggcc ggccagccaa aagacgatga ttccaccagg tagcgcgata
     4081 ccgatcagac ccttggccag gaagccgaaa cccgccgcgg cataggccag cgtcagcaca
     4141 agccggtagc gctcgccacg ctccatcctg aagaccgccg cggcgcccag caccaccgtg
     4201 gcggtgatcg cggcggcaac ggtcatatcc atgttggcgt actgcccgcc accgacaaaa
     4261 aacggcgcgg tcaccaggac cagcgtggcg agaatagcga cacggcggtc ggcgaaagtc
     4321 ttgatcagcc agaagaacat ggcggccatc aaggtggcgg ccagcaccga gcacagcctg
     4381 gcagcccatt cattcacacc aaacagccac agcgacaact cggtgaccca gtagaacaag
     4441 ggtggcttgt ggaagaatgg caggccgtcc agccgcggca cccaggcctg accgacgtcg
     4501 agcattcccc acgccacgcc cacgtagcgc ccttcatccg gcagcatcaa cggccgcgtc
     4561 caggtcgtgg ccgacaccca tagaaatgtg atgatcgcca ccagccacgt ggatcgaatt
     4621 tccagcatcc tggaggtcag ttgcaccaac cgattcataa acagatttcc cgaatggagg
     4681 ctgagtcccc gacgggaatc aagcctggta tcctaagttg gaggttcgtt gaacaataaa
     4741 tcagccgaga tcggcaccag tgcggtattt gacgctagcg ggaacaaata aaaccgatgg
     4801 cttcttggtc tgggcaaggc gcaaaccgca gcgatagcct gtgccaccga gaggatttgc
     4861 aacgccgccc aggccgaaaa gacgcggcat tatgttccag acagcgccaa acactgcaca
     4921 aggcaggtcg cgaatcatca agcagcttga tcccgatcgc gcccggacaa gccgttggcg
     4981 tcggccggca cgcccagccc cgtcccgatc cggtccttga tcaggtaaac agggcgcccc
     5041 ttgacctcgc tgaagatgcg cgccacatat tcacccacga cgcccagtga catgagattc
     5101 acgcccgcga aaaagaggat cacggcgatg atggttgccc agccctggac cacgtcgccg
     5161 tatatccagt ggttgaccac gacatacaac ccgtaggcga acgacagcag cgacaagccc
     5221 atccccgcta gactgaacat ccgcagcggc catgtcgtga aggccgtcaa gccatcgaac
     5281 gcgaagcgca ccagtttgaa cggtttgaag gtcgtcgtcc cgtgcaggcg cccgggcggc
     5341 gtgtagtaaa aaggttcgga accgaacccc acccaggcga acaggccctt catgaagcgg
     5401 tcacgctcgg gcaacatccg cagcgcgtcc accacccgcc tgtccatgag acgaaaatcc
     5461 ccagcgttct ccggcacttg cagcccattg gaagagcgca tcaaacggta aaagagtttg
     5521 gtgccgaccc gcttgaacag ggattcatcg gcgcgtgtct cgcgaaccgc atagaccatg
     5581 tccaccccat ggcgccagcg atcgagcatt tgtggaatga gcgccggcgg atgctgcagg
     5641 tcggcatcga gacacaccac cacctggcca tgcgccgcct cgataccggc actgagcgcg
     5701 gcctctttgc cgaaattgcg cgacagacgc aaataaacga tttcagggtg ggtatcggcc
     5761 cacgacgtga acaggcccga cgtggcatca gtgctaccgt cgtcgacgac gatgatctcg
     5821 tagcgtggcg ccagggttcg cagcaccgcg atgagcgccg gcaacagcac cctgagattc
     5881 gcctcttcgt tcaggctcgg caccacgcaa ctgatcgagt cgggtgaacg aacgacacct
     5941 tcctggacag tcgtgttgtc tgcggccgcg aggggtgggt taggcacgga acagcatctc
     6001 ggcaatggat ggatgggcga aaagagggga acggaagcga ttattgcaca ttcacttgcg
     6061 attccactta gcacagacac agcgtagacg gtagagaggg atttgactag ggttgtggat
     6121 ttttataaga ttcattttaa atatatctta aaggctcatc atgcccgagg ccgcgctctg
     6181 taccgaagaa gaagtcactc gtctggtcca tggtttctat ggtcgcgtcc gcgccgacag
     6241 ccaattaggg cccattttcg acgcgcacgt gggcgattgg gacactcatc tggccaagat
     6301 ggtgcagttc tggtcctccc tgctgcgagg caccggcacg taccgcggcg cccccatgcc
     6361 cgcgcatatc gccctgccag ggctgaacgc cggcctgttc cgacaatggt tggcgttgtt
     6421 ccacgacacc acacgcgaga tacccaaccg gccgttcgcg gagcgggccg aggaattcgc
     6481 gcatcgtgtc gcacgaagcc tttggttcgg gtaccagatc agccgcgagc ccgatcgcgc
     6541 accgctggag ctcaccgatg acgagtatcc ccagcacgct tgataacagc atgcccacca
     6601 ccaccgctcg cccccaatgg cgggcattcc tgagcctggg cttccgccca ttgtatttcg
     6661 cgggctgcgc ttgggcggcc gtggcgatcg ccatctggat cttcgccccc ggactcttga
     6721 agcaaccttt aggcggcctg gcttggcacg cccacgaaat gctgtggggg ttcgtggcca
     6781 ccatcgcggt tgggtttttg ctgacggcca gcgccaactg gaccaaaacc aaccccttgc
     6841 atggctggcc gctggccgtg cttgtgtcgc tgtggctggt cgcccggatc ggctatctgg
     6901 cgggcgggcg ggccgcattc cttctcgccg gcgcagcgga aaccgcattt ttcgtcgtgg
     6961 ccggggtggc ggtgttgcgc gtcgtgatca tggccggcag ccgccgcaac tacggcatcc
     7021 ccctgctgct gttcggcctg gccaccgcca atgtcctgta tctgcgcgcc gcgcagcaag
     7081 gcgattatgc cgaactgatg cgtcgattcg atctgggcct gctgtgcatg gctgtcatcg
     7141 cactgctgat cgcgcggcgc gtcatccctt tcttcaccat gcgcatggtg caaggcctga
     7201 gcatccctat gaacacacgc agcggacagg cgcagatcac attgggactc gtcgccatcc
     7261 tgactggcat ggtcggcttt tacctggtca tggccgccgc cctcgccgcc accggcctga
     7321 tcgggctcta ccagatcgtg gcctggcgtc ccctggcctc gcgccaccgg cccatcttat
     7381 ggatactgta tctgggctat gccatgctga gcctggggct ggtatccgcc gcgatctact
     7441 tggtaaaccc cgtcccgggt gttctggcac gggctgccac ccatgtgcat atcatcggcg
     7501 tgggcggatt ttccattctc atcataggca tggtcacgcg caccgcactg ggtcacctgg
     7561 gacgcccact gaagctggac gccagcatgc gcgtcagcta cctgaccctg atcatcgcaa
     7621 cagcgctgcg gctggccgcc ctgcctgcca ccggcccggc caacctgtgg ctgttgcggt
     7681 tggccgccgc cttctggatc gcgaccttcg tgttgtatct ttggcgcttc gcacccatgc
     7741 tgatccgccc gcgcccggac gcggcgcgat cctgacgagg ggcggccatg cgactcacgt
     7801 cgatgacgga ctatgcgata cgcctgttga tgtacgtcgc gtgccatccc gggcgcctat
     7861 gcacgatagg cgagatcgcc caggcctacc atatttccga gccgcacttg atgaaaatca
     7921 ccaatcgact gagtcaatgc ggatggatag agaccgttcg cggcaggaat ggcggcatgc
     7981 gcctggcccg gacgcccacc gacatccgcc tgggggcggt gatgcgcgac acggaaacgg
     8041 atttcgaact ggtggagtgt ttcggccccg gcagcacctg cacactgacc ggacagtgcc
     8101 gtctgacggg catcatcgac ggcgcgctac gggcattcct gcggcatatg gatgactaca
     8161 ccctggccga catcctgccc gcggcgtcct accccatttt tctggatacc cacaacgcca
     8221 cggtgtccca ggcccggccg aagaaccccg cctgacccac gggatgcagt gccaccagcg
     8281 gcagcgtgac cgtgcgatca cccacggttt ccgtcaggga cgcgacctgc gcatccgcgg
     8341 cgatgggggc gaccaacggc cccttggtgg tgaccttggg cacccccaag agcttcgcgc
     8401 cacgcccgac cgtcaggtac acatcgtggg tgaaacccac tttgacctga tcctgctcgc
     8461 ccttccagac ccttggcgtg gcgaccactt gttgcttgcc atagagcttg acggtgtcga
     8521 aattgccaaa gccccagttg agcagggttt cgctttcctg gactcgcgcc ttgctcgagg
     8581 gcgtgcccag caccaccgag atgagcctac ggtcaacagt ggccccgggg cggtgcgccg
     8641 acgcgatgag gcagtacccc gcttcagcgg tgtgcccggt tttcaatccg tcgaccgacg
     8701 ggtcgatcca cagcagcttg ttgcggttct gttgggtgat cttgtcgtac gtgtactgct
     8761 tgatcgtgtc aaaacccacg tacaacttgg gataatcctg aatgagatgg cgcgcgagtt
     8821 ccgacagatc gcgcgcggtg gaatacgttt cggggctggg caggccatgc gggctggcga
     8881 aatgcgtgga tttcaggccc atatgcgcgg ccatctggtt catttccgcc acaaaggttc
     8941 cgacgctgcc ggcggttgcc tcggccagcg ccacggcggc gtcgttgccc gaatcgacca
     9001 gcaaacccgt gagcaattgc ctgatggtga cacgatcgcc gggcttgagg aacatcttgg
     9061 agctctcggg atccaccttc caagccaggg tcgacaccgt gaccgtctgg tccatgctga
     9121 gctggtggtc gtgaatggcc ttgaaaacca agtaggccgt catgatcttg gtgagcgacg
     9181 ccggctggat gcgcgcgtcg gcattgtgct cggcgatgat ttcgttgctt ttggcgtcca
     9241 gcagcaacca tgcgttggcg tcgagcgccg gggcgggaat cgacagcgca aacgcccggg
     9301 acgccaccaa caggctcgcg cccagaccta gtatccattt cttcatacag agactttctt
     9361 aacccttcgt cttttcgcaa gccgccaccg gagataggct ttgcggtcac cttcttcgtg
     9421 caggcgatgt acccgcacac gcagtaagtc tttgcgggcg atcaggccca gcaactgatc
     9481 agtgtcgcga tcgacgatgg gaatgcggcc cacacccgtg accgccatcc tgtcggcgat
     9541 gcgccccgcc atttcgtcag gatatccgat caaaagttcc tgatcccgca aaatttcctg
     9601 caatgtcgtg gcgccgcgat cattcccgtt ttcgcggtcg accgtccagg ccaggctgtc
     9661 agcccgcgtg accagcccca gcacccgctg gccatcgtcg accacgggat agctgctatg
     9721 gcggaattcg gtggtcgtga aaaactcgat ggcctcggcg atggtcatgg tgccgggcag
     9781 tgtcttgacc gacttggtca tgacactgcc cgcccgttcc agatcgaacg cgtcgacatg
     9841 atactcccga gtcaggtggt gcccgtggcg cgcgactttc tctgtgagaa tggagcgctt
     9901 caacaacagc accgtcaggc cataggcgaa caggcacgcg gtcaggacag gcagcagcac
     9961 ggaatagttg ccagtcagtt cgaccgcgaa caaggtggac gtgagtggcg cgcgcatggt
    10021 gccacccatc atggcggcca tgccgaccag agcccagaaa ccggggtcgg catgcggaaa
    10081 ccatggcgcc agcacggcgc cgatggtgcc tccgatgatg agcaacggtg cgagcacacc
    10141 gcccgacgtg ccggacccca gcgccacgga ccagatgatc accttgacga ccagcagcag
    10201 aaacgcagcc ttcagggcga cgtcgccggc cagcagatgg cggatgttgt cgtaccccac
    10261 gcccagcgcc gccggttcga tcaggccccc caccccgacc accaccccac ccaatgcggg
    10321 ccaccacatc cagtgcaatg gcaatttttc gaacaggtct tcgcaggcgt agacaatggc
    10381 cgtcagcagg cccgagccga agccggcaac cacgcccatc agagcccagg ccgcgacatg
    10441 ccaggtcgac aacgtgacgc cccccacata gggaaagatg ggggccgcgc cgggcatgaa
    10501 gaccgggcgc agcgcctgcg cgacaatggc ggcgatggcc accggcagga agctgcgcgg
    10561 tttccattcg aacagcagca attccaccgc cagcaagatg gaggcgacgg gtgtgccgaa
    10621 gaccgctgtc atgcccgccg ccgcccccgc gaccagcaag gtcttgcgct cgctggagct
    10681 catgttcagg gtctgggcca gcaacgaacc caacgcgccg cccgtcatga tgatggggcc
    10741 ttccgcgccg aacgggccgc ccgtaccgat ggaaatagcc gacgacaacg gtttgagtat
    10801 ggccactttg ggctgaatgc ggctcttgcc tatgagaatg gcctctatgg cttcgggtat
    10861 gccgtggcca cggatcttgt cagagccata gcgcgccatg agcccgacga caatgctgcc
    10921 caccacaggc acgaacacca tgtaccagcc caggggtgag ccgtcgatcc ctttgttgac
    10981 gaaggaccat tggccgtaat aggccacgtt ggtgcacaga tagagcagct tgaaaagcac
    11041 ccaggaagac aggtcgccca gtatgccgac aaccaccgcc aagcccatga ggtacagaac
    11101 gcgccgatca gtcgtgaagt cgcccagctt gggtatggtt gttttggtgc tgggggacaa
    11161 gaccttgggt gagcgcatcg agggttctgc ggttatgcgt tgaatgaacg aatcctgaaa
    11221 tgaaaggaag cgacattgga acggcaccga ccacatggcg aaggcgccgc tgcgttgaac
    11281 ctgttttata tcgtagcacg atataaataa cgggagcaat gaaaccgtct ttcccgccag
    11341 cgccgaccag cgtgctgtct ggtgaatgcg actgcccggc ttcgaaagca cgagccaacc
    11401 aggcagcaac cagccaaacg gggggctgtg tttccaggga acggggttag tgcttcggaa
    11461 agaggctaat gctcgaaacg accaaacaac ctgcccatga gcggccggat cgcgccaagc
    11521 tccttcaaat gggcgctgga cagattctcg agcttgcttt cggccaggcg tgacagcgac
    11581 acgcgcacgc gccggcgatc attggggtcg cgcaagcgtt ccaccagacc catccggacc
    11641 aatcggccga ccagctcgac cgcgctatgt ggacggatca tcagatagtc ggcgagttca
    11701 cccacgctgg gggcgtcgga ggtgctcatc gccttgatcg cgagcagcgc ctggtgctgt
    11761 tgcggcatca ggccggccgt ggcggcttcg gtttcgctga acgcaatgaa ctttcgcaac
    11821 gcttgccgga acgtggcaag cgcctggtaa tcggcgggcg tcaattgccc ggcgtcattc
    11881 gtatgcgcgg gacggtcggt gagtttcaca actcgattct aaaccatgcc gctgcgcaat
    11941 cgcgcattgt ggggccaccg acgagagccg gcgcgcccca ccccacggga tcacggttta
    12001 tgctcacggc tcacattcgg tcgcaacgga tggtgatcgg atggcggcgc aatgcgtcca
    12061 tgacctcgtc ggtcaccgag ccatccacgt cggagatgac atagccgatc tggccacggg
    12121 tctgcagttg ctgggtcacg atgttcagcc cgtgttccgc gagcaggctg ctaagcgcac
    12181 ccagcgcgcc gggcacgttg cgatgcacat gcagaatgcg cgccgcgccc gacgattcga
    12241 cgtaaggaat ttccgggaaa ttcaccgcgc ccttggtcgc accgccctgg gcgaagcgaa
    12301 cgagtttctc ggcgacctcg cggccaatgt tctcctggga ttcctgcgtg ctgccaccga
    12361 tgtgcggcgt gaggatcacg ttgtgcatgc cgatcaaggg actggccagg ggttcgtcca
    12421 cgctcttggg ttcagcggga aagacatcga gcgccgcacc cgacaaagac ccgttttcca
    12481 gggctccacg caacgcatcg atatcgacga cggttccgcg cgacgcgttg atgaggatcg
    12541 caccgcgctt catgaccgcg atggcggcgg cactcatgat gttccgcgtg cttttgccgc
    12601 ccggcacatg cagggtcaca acatcggact gcgccagcag atcattcagg taggcgcagg
    12661 cgcgggcgtt gcccaacggc agtttggctt cgatgtcgtg aaagatcacc cgcatgccgg
    12721 ccgactcggc cagcgtcccg acctgggatc cgatattgcc gtagccgatg atgcccagcg
    12781 tcttgccgcg cgcctcgtag gccccttccg cggtcttgtc ccagtggccc tgatgcaccc
    12841 gggcgttctt ttccggaatg cggcgcagca gcagaatggc ctcccccagc accagttcag
    12901 ccaccgagcg tgtgttggag tacggcgcgt tgaacaccgg cgttccgtgc gccatcgcga
    12961 agtcgagatc cacctggttc gtgccgatgc agaagcaccc gaccgcccgc aggtcgggca
    13021 atccggcaag gagctccgcc gtcagatggg tgcgggatcg gatcccgacc agcgccgcgc
    13081 cgcgcagcgc gtcgggcaaa tcggcgggcg gcaacgacga cgcataggtc ttgatgtcgt
    13141 cgaagcccgc cgcttcgaac acctcgcggg cgctggcatg gatgttttca aacagaacga
    13201 tacgagtcat gagagtttcc gaaacacacg gcaaagggtc tattgtgacg catagcattg
    13261 gtgaaaaccc tgacagccat gctgccgcca ggggcaaagg cgccgtcgct cttatagcgt
    13321 gaccgttccc tcgatgcacg tgctgacgtc gcccccaatc cagatgtccg gcccatccga
    13381 gtgaacatga acccgacccg cgcgacccag cgccgtgccc tgcgcggcca cgtacgaggc
    13441 cggcgccctg tcggtgccga tcagccattg cgccacactg gcattcaggc taccagtgac
    13501 gggatcttca ggcacgccca gatcgggcac gaaggcgcgc acttcaaact gtgcgtcgga
    13561 accgtcgaca gcctcatccc acggggcgac cacgcccagc ttgatgccat tgaggcggac
    13621 aaagtcgggc ttcaggttca ggacctggcg gcgcgacccc agcaacaccg ccagccatcc
    13681 gggtccgtta tccacccact gcgcatccag cacgtcgtcg tgggaaattc cgagcccgtc
    13741 agcgatacgc cgcaacagcg gcgcatcgac tggtccggtg cgcaccatgg gcggtgccgc
    13801 gaacgccacg cgccggccat cgcggcgcag cgggatcagc cccaccgcgc attcctggac
    13861 gatgcgctcg cctcgcggcc ggcctccggt ggcgagccag acatggcaag cccccagcgt
    13921 ggggtgcccc gcgaacggca attcggcctg gggcgtgaaa atgcggaccc gataatccgc
    13981 ttccggccta gtgggtttca aaagaaagat ggtttcgctg agattcgtcc agttggcgaa
    14041 ggcggccatc tgctcgtcag ccaggccatc ggcctccacc accaccgcca gggggttgcc
    14101 tttgagcggc gtgtcggaaa agacatcgac ctgcttgaac tcgaacgtgc gcatgatccg
    14161 accctcgcca aatcagaaag gatactacgc acgcgacatc cctggcggcg cggtcgggaa
    14221 caggctcagg cttcatgcaa ggccgtgtcg gcttccccgg cctgcgccgc caggacctcc
    14281 agcaccgcct cgcccttgcg ctgcatgtgc acccgcagaa tgcctgccag acgagggccg
    14341 tcatgcgcgg caagcgcctc caccatctgg atgtgctcgt gcatcgccgc atcccatttg
    14401 tcgtggttca gattcgagcg aaagcgcaga ttctgtatcc gcaggttgag cgtcccatag
    14461 acttgggaca gcaagtgatt gcgcgccgcc cggttgatgt gatcatggat ggcgcaattc
    14521 agacgatagt aagagggcaa atcctggcgg gcatggcagg cccgcatctc gaaagtcagt
    14581 gcgcggatct cggcgatttc ggtgtcggtc acatgctggc aagccagctc tcccgacaac
    14641 gcctcgagcg cccccatcac ttgaaagctc tcgcggacgt cgtcaaccga caacgccacc
    14701 actcgcgccc cgcgattggg ctgaatccac accaacccct gggcggagag ctgacgcaag
    14761 gcctcccgca acggcgtgcg cgacaccccc aactgatcgc atagcgcgcg ttcgttcaga
    14821 cgagcccccg ccggtaacag cccttcgatg atcatgtcgc gtagttggtt ggcgatcgat
    14881 gccgggagcg agcggcggtc caccgcctgg ggctctaggg aagcgggaag aaaagtgggc
    14941 tcgttcatgg cgtcatcgta tcaaccagaa ggtggacgtg gaagcattca ttggcggcat
    15001 cttgcctatg ttctcagaaa atatgaattt cgtataccaa tatactttca ccatggatat
    15061 tcttaagaaa gcaggcctgt ggaagccctc cttagcctct ttcatgaacg attatttccc
    15121 aatttcactg ctctatgtac attgaattaa taggcattta cccgaagaga ccctctgtag
    15181 cggttgcatc atcgcgggaa gccggtatag tgccaatact atatggtttt ttgtatacca
    15241 atatataaaa ctgaccccat gattccgcta gacacccatc catccggccg gcatttcctt
    15301 cagattccgg gcccaaccaa tgttccggat cgaatccgca gggcgatcag ccaggtcacc
    15361 atcgaccatc gcgggccgga gttcggcacg ctaggcaaag ccgtgctggc cggcatgcgg
    15421 aaaatcttcc agacagacgg tccggtggtg attttccccg cgtcgggcac cggtgcctgg
    15481 gaagcagccc tggtcaacac gctgtcaccc ggcgatcgcg tgctcatggc agaaaccggc
    15541 cagttcgcca cgctgtggaa gaacctggcc gcccgactcg gcctggaggt ggagtttctg
    15601 cccggggact ggcgacaccc ggtggattcg tcccaggtcg aagcccgtct ggccgccgac
    15661 acgcagcatg acatcaaggc ggtctgcatc gtccacaacg aaaccgccac cggcgtcacc
    15721 agcgacatcg ccgcggttcg ccaagcgctt gatcgcgcca cccatcccgc gctcttcatg
    15781 gtcgacacga tttcgtcgct ggccgccatc gattaccggc atgacgagtg gaaagtggac
    15841 gtcaccgtgg ccggctcgca gaaaggcctc atgctgcccc ctggcctctc tttcaatgcc
    15901 ctcggcgcca aggcgctggc cgcttccggc acagccgcgc taccgcgttc gtattgggac
    15961 tggaagccaa tgctggacct caatccgtcg ggctactttc cctacacgcc cgcgaccaat
    16021 cttctctatg gcttggccga ggccgtggac atgctgctcg acgaggggtt ggacaatgtg
    16081 ttcgcccggc acaaacgcct gggcgaggcc acgcgccggg cggtacgggc ctgggggctc
    16141 gaggtcctgt gcgccgacga aaccgcccac agcgcggccc tgacggcggt gctgatgccg
    16201 cccggccaca gcgccgacgc cttccgcaag acggtgctgg agcgcttcga catgtcgctg
    16261 ggccaggggc tgggcaggct caaggaccgg gttttccgca tcgggcacct cggggacatc
    16321 aacgacctga cgctctgcgg cgtgctggcg ggcattgaaa tggggctctc cgccgccggg
    16381 gttccgcatg acaaaggcgg cacgcaggcc gccatcgatt ttctggcggc aaccagcgcg
    16441 acgccccggg aggcatagaa acatgtccga ctccgattca gaaagcagcg cctggcacgt
    16501 ccctcccagc ctgtatgcgc gccagcgcga ggtggcgttc accctgccag gcaggcccgc
    16561 ctccgtctac gtgccgatgc gcgacggctg tcgcctggcc cttgattact atctgccgca
    16621 aaccgatcgg ggcgggccgc gcgccgagga tcggttcccc gtcattgcgc tgttcacgcc
    16681 gtactatcgg cgcttcgcgc tggccgatgc gtctctgggc acagagccaa ctcccaacgc
    16741 ggggaagtac cgcgattact tcgtgccacg cggctatgcg ctggtcgtgg tcgacgtgcg
    16801 cggaacggga gcgagcttcg gcacgcgcga cagcttccgc tccccgcgcg agcgggacga
    16861 ctaccgcgaa atcgccgact ggatcgtggc gcagccatgg tgcaacggcg tcataggcgc
    16921 cacgggcatt tcgtacctgg gcgccgcctg cgactttctc gccagcaccg ggcatcccgc
    16981 cgtcaaagcc atcgcgccga tctcggcggt atgggacacc tacagcgatc attactatcc
    17041 cggcggcctg tttctgaacc gcctctccca gacgtacgac cggctcatgg tggcgctcga
    17101 ccacgaccgc cgcgacctgc tgggcgagtt cggctacttc agcgacccca actacgcggg
    17161 cccgcaaccc gtggatgacg acaccgacgg gcgggcgtgc cgcgcggccg tgcatgggca
    17221 tctcggcaat ttccgcatgc cggatttcat caccgggttc gcgtgccgcg acgatgcgct
    17281 cccctacgac cccgctttca cctccgccag catcagtcct tatcactacc gggacggcat
    17341 tcgtcccgac gtggcggtgc tgtcggtgtc gggatggatg gatggcgccg gttacgccaa
    17401 cggttcgatc gcccgtttcc tcaccttgaa cggcaacccc gggtacctgc ttctggggcc
    17461 ctgggatcac ggcgcgcggg tcaacacgtc gccctggcgc aagagcgtca ccccgcaatg
    17521 caacccattc gacgaaatcc tgcggttctt cgaccattat ctgtgcggca tggacaccgg
    17581 tctgcggcgg gaagagcccg tgcactattt cagtgtgcat gaagaagcct ggcatgcggc
    17641 ggcggaatgg ccaccattgc ggggctccgc caccctgagc ctggcctcgg gccaacgtct
    17701 cgccgggcca gccgaggccg acgaggccaa taccggggcc gatgggttcg acgacgtgaa
    17761 cgtggatttt tccttcggca ccggcaacgg cacccgctac gaccgcatcg ccggccacga
    17821 ctcccgcgac taccacaccg actggaaaca acgccaggaa accctcttca gctacaccgg
    17881 cgacccattg cccgccgatg ccgaactcac tggccacgcc atcgccgatt tgtggatggc
    17941 ctccagcgag cccgatgccg ccattcatct ttacctgtcg gaagtgcttg aaaacggtga
    18001 agtccactac gtgacggaag gcctgctgcg cgccctgcac cggcatgagg ccccggcacc
    18061 cgccgcgtac cgggcgacct ggcctttcag aaccttctca cgcgcggacg cttcgcccat
    18121 gccggtcgac acaccccaga acctgcgctt cgccctgctg ccggtctcgt ggctgttccg
    18181 gcgcggcagc cgtatccgtc tttccatagc cggtgccgat gccgaccacg tggcgcaagc
    18241 gccacatggc cggccgcccc gcctgcgcct ctggcgtggc gcgcggcaac cctcgtcact
    18301 cgcgctgccc ctgcggttca aaccgtgacg caatccacct aggagtgatc atgaaacgac
    18361 tgactcaact ggctctcgca ggcgtggccg tactcgccct gcagacgccc gccatggcgg
    18421 ggaaaagcga caacacgatt gtctggtcga ccgcatcgga agtggacacg gcagacgttt
    18481 actaccagaa cctgcgtgaa gtggtcatcg cctcgctcaa catgtgcgac tcgctcgtgc
    18541 acctcgaccc gttcacgcac aagtatgaac ccctgctcgc gacgagctac aagtgggtcg
    18601 acccccacac cctggatttc acgctgcgga aaggcatcaa gttctggaat ggcaaggaat
    18661 tcggtgccaa ggacgtggcc tatacactca accatgcgcg ccaaccggac agcggtgtgg
    18721 tgactcgtct ggccgtcgac tggatcaaga acgtcgaggt cgtcgggccc tacgaagtgc
    18781 gcatcatcgc caaggatccc acgcccgccg cgctgaccta tctggctgac gaaaccccca
    18841 tctatcccga tgggcactac gacagcgcac cagccatccc gggcgccaag gggcagacca
    18901 gaaaagacta cggtgccgtc aaaccggtct gcaccggtcc ctacatcctg aaggacttcg
    18961 tgccgggctc gtcgttcaca ctggtcaaga acacgcacta cttcaagggc ggaccgaaag
    19021 ggcagcccaa ggtcgacaaa ctggtcttcc gcaccattcc cgacaccgac tcccagatgg
    19081 cctcgctcat gacgcacaag gtggattggc tttggtccgt gccgccccag aacgccaagc
    19141 agctcggcgc catgcctggc atcacggtga agtccgcccc caccacgcgc ttgtcgtttc
    19201 tttcgctcga cgccgcgggt agatcgggca agaaccctat gcagaacctg aaagtgcggc
    19261 aggccatcgc ccatgccatc gaccgcaagg ccatcgcgca cgacctggtc ggacctggcg
    19321 cgcaggtgct gaaatccatg tgctacccgg cgcaattcgg ctgcacggaa gacgttcccc
    19381 aatacaacta cgatcccgcg ctggccaaga agctgcttgc cgaagccggc taccccaatg
    19441 gcttcgatca ggtcatctat gcctatcgcg accgccccta cacagaagcc gtcatgaact
    19501 atctgcgcgc ggtcggcatc cgcaccaagc tcagattcct gcaatggaag gccctgcggc
    19561 cactcatcga caaaggccag gtggccatgg cccagttgac ctggggttcg caaggcatga
    19621 tggacgcgtc ggcatcggtc agcaattact tcgaattcag ccccgacgac tatgcgcgcg
    19681 acaagcaggt gcgcgactgg ctggtcgcgg ccgaccacac aaccgatccc gaagcgcgca
    19741 aaacgctgta caagaaggcg ttgacgcgta tcgccgacca ggcctatttc attcccctgt
    19801 tcacgtacgc acgcatctat gctttcgact ccagcctgga ctatccggtg accccggacg
    19861 aactggcgca cttctatctg gcgcattgga aatgattctc cgctcgtcct ccttgagcgg
    19921 gccaggcatg gcgcgctttc ggcacccgcc acggcgggtc cggccatgct aggtttcata
    19981 ctcaagcgcc tcgccctgag cgtcctggtc gtggccgtgg tgtcggtcgt cgccttctcg
    20041 ctggtgtatc tgtcgggcga ccccgccgcc gccatggccg gcgagggcgc gacgcaggcc
    20101 gacgtggcag ccgtgcgcgc cacctatggg ttcgaccgcc ccctgtacgc gcagtatgga
    20161 tcctggctgc gcaacgtcat gcatggtgac ttcgggcggt cgtactatct gcgggagcc
//
LOCUS       DASTNK010000130        10107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_100075, whole genome shotgun sequence.
ACCESSION   DASTNK010000130 DASTNK010000000
VERSION     DASTNK010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10107
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100075"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1561
                     /locus_tag="VFR20_09785"
     CDS             <1..1561
                     /locus_tag="VFR20_09785"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0230852.1"
                     /translation="SQTMLDLSKDTLDPRLAKRAFQMASAGRDMSEALDAAREWVLLA
                     PDDPQAIATSLAVAASNGDTSGLSAALWDRIEKAQDKNVAIAQAAAIVSKMENKKLAF
                     EVLDGALRDPVRSLPMAHIALADAAWAAGDSERALTEAQTAMKMAPDSEPAAQRVLEY
                     GMKVNPSQAISVTKAFIAGHPDSRKLQLMLVNHLVGLHETAQALDILHEMRRRTPEDF
                     DLLYTEAEVDFRAGDYEQAKALLGNYIDVQTQRGKSFNVNATNAQSDVSDARLLLVQI
                     AEKQNKLADAIEQLRLIDDPTVRFQAHLHMAVLQARMGNIPVARKTLANLKPDSRHEK
                     AVIALTLAAIYRNSGRTDDALQVLVDADKALPDTSEIKYDLGMMYARQGKLKQFEALM
                     RRVIELDPDNADAYNSLGYTYADQNEHLDEARDLLDHALELDPGNPFILDSMGWYLYR
                     VNDLHGALDYLERAYRKLPTADVAAHLGEVLWKLRRHEQARKVWQEGYKQDPHDDTLL
                     KTLKRFRVTFK"
     gene            1558..2136
                     /locus_tag="VFR20_09790"
     CDS             1558..2136
                     /locus_tag="VFR20_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEU0230853.1"
                     /translation="MTRRGFGRRLARGGMAAALAVLLAACAVPPKLVGRGGPSFSRNG
                     RFAVNVSGGANGPHAVQGGFSWLDEGGELRLDLANPLGSTLARIEVTPAGATLRRADG
                     SREAAQNADALAAQVLGTPIPVEGLRDWLRGRTDGKAHNLNTNASGRPESFVENGWEV
                     RLARYDAVGPRLLVMQRDDAGARISVRLAVDS"
     gene            2157..3143
                     /gene="ispE"
                     /locus_tag="VFR20_09795"
     CDS             2157..3143
                     /gene="ispE"
                     /locus_tag="VFR20_09795"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEU0230854.1"
                     /translation="MALYDVPAPAKLNLFLHVVGRRADGYHLLQTVFRFIDLNDSLDF
                     DARGDGRISCHNPGLTDPSDDLIVRAARALQQAAAIKTGAHIVCRKTIPMGGGLGGGS
                     SDAATTLIALNRLWGAGLSRAELMRLGLSLGADVPVFVFGQSAFAQGVGEKLAAVELR
                     ERAYIVAQPFRNVPTQAIFQSPELTRNTACVKIAVFSNWQKSSGTDGVGGRGGNGGTD
                     SGGDGTLGKGYGQGLALFGRNDLEPVACAMYPEVGRLAGRFRECGVVARMTGSGGCFF
                     AEFVNMDQARTCLREVTDTIPSHSEGGEAPVIKRSWLCQGLTEHPLRHWISS"
     gene            3145..3221
                     /locus_tag="VFR20_09800"
     tRNA            3145..3221
                     /locus_tag="VFR20_09800"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3179..3181,aa:Gln,seq:ttg)
     gene            3350..4300
                     /locus_tag="VFR20_09805"
     CDS             3350..4300
                     /locus_tag="VFR20_09805"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEU0230855.1"
                     /translation="MSQDRFMIFTGTANIRLAVDVVNHLDMSLGKMTVGRFSDGEVMV
                     EINENVRGKDVFVLQPTCAPTNDNLMEIMVMVDALRRASAGRITAAIPYFGYARQDRR
                     PRSARVSISAKVVANMLQVAGVDRVMTMDLHADQIQGFFDIPVDNIYAGPVLLGDIWR
                     RNYKDLMVVSPDIGGVVRARALAKQLEADLAIIDKRRPRANVSEVMNIIGEVDGRTCI
                     IMDDMVDTAGTLCKAAQALKDRGAVRVFAYCTHPVLSGGAVQRITESALDELVVTDTI
                     PLSEDGLACGKIRQLSCASLLGETMLRISTAESVSSLFVD"
     gene            4404..5033
                     /locus_tag="VFR20_09810"
     CDS             4404..5033
                     /locus_tag="VFR20_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEU0230856.1"
                     /translation="MKFNAVSRSEQGTSASRRLRHAGRVPAIVYGGQTGPVSIELDHN
                     EIYHALRKEQFHASILSMELNGKSEQVLLRAVQWHPYKQQVLHVDFQRVDASHAINTK
                     VPLHFLNAEVSPAVKLNSAVISHVLTEIDIVCLPANLPQFIEVDLSQILAGATLHLSD
                     VTLPIGVTYAAHGGDANPVLAAALVKAGKAEEEETPAAPAGETPAAPAE"
     gene            5235..5831
                     /gene="pth"
                     /locus_tag="VFR20_09815"
     CDS             5235..5831
                     /gene="pth"
                     /locus_tag="VFR20_09815"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU0230857.1"
                     /translation="MSEPIRLIIGLGNPGPEYETTRHNAGFWLADHFADDLSGVFTLE
                     KAFSAWVAKGRFEGAAIVLAKPSTFMNRSGLAVGTLAHFYKLAPEQVLVLHDELDLLP
                     GQVKLKQGGGHAGHNGLRDIQRVLASPDFWRLRVGIGHPRTLGLTQQVAGFVLNPPKR
                     DELHKIESVIDRCRAVLPALLRGEFSQAVNQLHEGNRE"
     gene            5838..6605
                     /locus_tag="VFR20_09820"
     CDS             5838..6605
                     /locus_tag="VFR20_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
                     /protein_id="HEU0230858.1"
                     /translation="MTDAVRSLGFRAELRDFLRFVARPSFGPRLPGRVAMHGWVEDWF
                     PSLRPGSLLRWAVVLWIVNLFFLGPLSLAAAMAGGAQSRLNLWNIPWVAALLWAPVVE
                     ELVFRYGLRHIAQALWLVPAALVAMLLGLRWYSVVLLVVVLAVCWIPVALGWRVARRP
                     LPWRWRRRYQRAFPWVFHVSCLLFAGAHLANFSYDSTPLWVMPFLVLPQWLTGVVLGW
                     QRMRRGVGASMLLHGIFNAGPLGMVWLVLHLAPHLMT"
     gene            6724..7815
                     /gene="ychF"
                     /locus_tag="VFR20_09825"
     CDS             6724..7815
                     /gene="ychF"
                     /locus_tag="VFR20_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU0230859.1"
                     /translation="MALQCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGVV
                     EVPDERLAALAGIVKPERIVPAVVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVH
                     VVRCFDDDNVVHVAGKVDPISDIGVINTELALADLATADKALQKNLKLSRSGDKEAAK
                     LVALLEKIVAALNEARAIRSLGLDEDSLAMIRQYCFITAKPTLYVANVKDDGFHDNPK
                     LQAVQDYAKTENAPVVAVCAAVEAEIADLDDADKHEFLADMGMDEPGLNRVIRAGYRL
                     LGLHTYFTAGPKEVRAWTMHIGDTAPKAAGVIHTDFERGFIRAQTIGYDDYIACKGEQ
                     GAKEAGKMRAEGKEYVVKDGDVMNFLFNV"
     gene            7887..8144
                     /locus_tag="VFR20_09830"
                     /pseudo
     CDS             7887..8144
                     /locus_tag="VFR20_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
     gene            8501..8812
                     /locus_tag="VFR20_09835"
     CDS             8501..8812
                     /locus_tag="VFR20_09835"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0230860.1"
                     /translation="MVFSLSVPTEILHTLGARLRAQRLAQALPQRDLAQMAGLSLGAL
                     RKLERDGQCSLETLVRVVQALGLAHELDDLFVLKRQSIAQMEQAEDVSQRQRAPRRKS
                     P"
     gene            8809..10035
                     /locus_tag="VFR20_09840"
     CDS             8809..10035
                     /locus_tag="VFR20_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HEU0230861.1"
                     /translation="MKQLTVHYCGWGEDWPLGRMADDGQALLFEYSPEALAQGLELSP
                     LHLKLRAQAYGGFPAHLQRLPGLVADSLPDGWGLLLMDRLFRQQGVRHPGPLDRLAFI
                     GDRAMGALRFVPSHDTDVQEPDWDLLVLAEESTHALAGEARAALRELALAGGSPQGAR
                     PKAVVRFDSTTGRVSTRSDAPGESWLVKFPAQGEHKEVCAIEQLYAELARDCGLEMPD
                     SHWFDLSPSLAAFGVARFDRGQGLRVPLHSLAGLLQVDFRLPGATDYTAFLRATRLMT
                     HDEREVEKAYARAVFNVLFHNRDDHPKNFAWCLGRDRRWRLAPAFDLTFSEGPMGQHH
                     MDVCGEGAMVGRPHLLRLAVEGGVQLKVAERIIERQLEQASDFPRRISAFPVRQATAQ
                     AMNSAIEACRGRLESR"
BASE COUNT         1764 a         3134 c         3260 g         1949 t
ORIGIN      
        1 cagccaaacc atgctcgatc tctccaagga cacgctcgac ccgcgcctgg ccaagcgggc
       61 gttccagatg gcgtcggcgg ggcgcgacat gtcggaagcg ctggatgcgg cgcgtgaatg
      121 ggtgctcctg gcccccgacg atccccaggc gatcgccacg tcgctggcgg tggctgcttc
      181 caacggcgat acttccggct tgtccgccgc gctctgggat cgcatcgaga aagcccagga
      241 caagaacgtc gccatcgccc aggcggcggc catcgtcagc aagatggaaa acaagaagct
      301 ggcgttcgaa gtgctggatg gggcgttgcg cgatcccgtg cggtcgctgc ccatggcgca
      361 catcgccctg gccgacgcgg cgtgggcggc cggcgactcg gagcgcgcgc tgaccgaggc
      421 gcaaaccgcc atgaaaatgg cccccgactc ggaaccggcg gcgcagcgcg tgcttgaata
      481 cggcatgaag gtcaatccgt cgcaggccat cagcgtgacc aaggctttca tcgctggtca
      541 tcccgattcc cgcaagctgc aattgatgct ggtcaaccac ctggtggggc tgcatgaaac
      601 cgcgcaggcg ctcgacatcc tgcacgagat gcgtcgacgc acgcccgagg atttcgacct
      661 gctctacacc gaggcggagg tcgatttccg cgcgggcgat tatgagcagg cgaaggcgct
      721 gctgggcaac tacatagacg tgcagaccca gcgcggcaaa tctttcaacg tcaacgccac
      781 caacgcccaa tcagacgtgt ccgacgcccg cctgctgctg gtgcagatcg ctgaaaagca
      841 gaacaagctg gccgacgcca tcgagcagtt gcgccttatc gacgacccca cggtgcgttt
      901 ccaggcccac ctccacatgg cggtgttgca ggcgcgcatg ggcaacatcc ccgtggcgcg
      961 caaaaccctg gccaatctga aacccgacag ccgtcatgaa aaggccgtca tcgccctgac
     1021 cctggcggcc atctatcgga actccgggcg caccgacgac gcgcttcagg tactggtcga
     1081 cgccgacaag gcgctgcccg atacctcgga aatcaaatac gacctgggca tgatgtacgc
     1141 gcgccaaggc aagctgaagc aattcgaggc gttgatgcgg cgcgtgatcg agctcgaccc
     1201 cgacaacgcg gatgcctaca actcgctggg ctatacgtac gccgaccaga atgagcacct
     1261 ggacgaggcg cgcgatctgc ttgatcacgc actggagctc gatcccggta atccgttcat
     1321 cctggacagc atgggctggt acctgtaccg cgtgaacgat cttcacggcg cgctggacta
     1381 tctcgaacgc gcctatcgca agctgcctac cgccgacgtc gccgcgcatc ttggcgaggt
     1441 gctatggaaa ctcaggcgcc atgagcaggc gcgcaaggtc tggcaggaag ggtacaagca
     1501 agatccccac gacgacacgc tgctcaagac tttgaagcgg ttccgggtga cgttcaaatg
     1561 acgcggcgcg ggttcgggcg ccgcttggcg cgtggcggca tggcggccgc gctggcggtg
     1621 ttgctggccg cttgcgccgt gccgccgaag ctggttggcc gtggcgggcc gtcgttcagc
     1681 cggaacgggc gtttcgcggt gaatgtcagt ggtggcgcca acggcccgca tgcggtgcag
     1741 ggcgggtttt cctggctgga tgaggggggc gagttgcggc tcgacctcgc caatccgttg
     1801 ggcagcacac tggcccgcat cgaagtcacc ccggccggcg ccacgctgcg gcgcgccgac
     1861 ggctcgcgcg aggccgcgca gaacgccgac gcgctcgcgg cgcaggttct tggcaccccc
     1921 atccccgtcg agggcctgcg cgactggctg cgcggccgca ccgacggcaa ggcgcacaac
     1981 ctgaacacca acgcatcggg gcggccggaa agtttcgtcg aaaacgggtg ggaagtgcgg
     2041 ctcgcgcgct acgacgcggt cgggccgcgc ctgctggtca tgcaacgtga cgacgccggc
     2101 gcgcgcatca gtgtgcgcct cgccgtcgat agttagatcg ctcgagtgcc tgactcatgg
     2161 ctttgtatga tgtgccggcg ccggccaagc tcaatctgtt tctgcatgtc gtgggccgcc
     2221 gggccgatgg ctatcatctg ctgcaaaccg tcttccggtt catcgacctg aatgattcgc
     2281 tggactttga cgcgcgcggc gacggccgga tctcctgcca caaccccggg ttgaccgatc
     2341 cctcggatga cctgatcgtg cgcgcggcgc gggcgttgca acaggcggcc gccatcaaaa
     2401 ccggcgcgca tatcgtttgc cgcaagacga ttcccatggg gggcgggctc ggcggcggat
     2461 cgtcggatgc ggccaccacg ctcatcgccc tgaaccgact gtggggagcg ggcctgtcgc
     2521 gcgccgagct catgcgcctg ggtttgtcgt tgggggcgga cgtgccggtg tttgtgttcg
     2581 gccagtccgc tttcgcccaa ggcgtgggcg agaagctcgc cgccgtcgaa ttgcgggaac
     2641 gcgcgtatat cgtcgcgcaa ccattccgca acgtgcccac gcaggcgatt ttccagtccc
     2701 cggaattgac aagaaacacc gcatgcgtca aaatagccgt cttttcaaat tggcagaaaa
     2761 gcagcggcac cgatggcgtt ggtggtcgtg gcggcaatgg tggcaccgat agcggtggcg
     2821 atgggacgct tggcaagggg tacggtcagg gtcttgcgct ttttggccgc aacgatctcg
     2881 agccggtggc ttgcgccatg tacccggaag tcgggcggct ggccggacgg tttcgtgaat
     2941 gcggggtggt tgcccgcatg acaggttccg ggggctgttt ctttgccgaa tttgtaaaca
     3001 tggatcaggc ccgtacatgc ctgcgcgagg ttaccgacac aatcccgtcg cacagcgaag
     3061 gcggcgaggc cccggtgatc aaacgctcgt ggctgtgcca gggtttgacc gaacatccac
     3121 tgcggcactg gataagcagt tgattgggga atcgccaagt tggttaaggc actggatttt
     3181 gattccagca tgcgaaggtt cgaatccttc ttccccagcc attatttttc aagcgggccc
     3241 atcggctggc tgaccgcagg ccggctgacc acaggctggt gctggcgtct tcgcggcgtt
     3301 ctggcagcgg ccttgccgtt ttaatgcaac gaaacctccg ccgtccatca tgtcacaaga
     3361 cagattcatg atctttaccg gcaccgccaa cattcgtctg gcggtcgatg tcgtcaacca
     3421 tcttgacatg tcgctcggaa aaatgactgt gggccggttc tccgacggcg aagtcatggt
     3481 cgaaatcaac gaaaacgtcc gcggcaagga cgttttcgtt ttgcagccca cgtgcgcgcc
     3541 gaccaatgac aacctgatgg aaatcatggt catggtcgat gcgctgcggc gggcttcggc
     3601 tggccgtatc accgccgcca tcccgtattt cggctatgcg cgccaggatc gccgtccgcg
     3661 ttctgcgcgg gtgtcgatct cggccaaggt cgtggccaac atgttgcagg tggcgggcgt
     3721 cgatcgcgtc atgaccatgg acctgcatgc tgaccagatc cagggatttt tcgacatccc
     3781 cgtggacaac atctatgccg ggccggtgtt gttgggcgac atctggcgtc gcaactacaa
     3841 agacctgatg gtggtctcgc ccgacatcgg cggcgtggtc cgcgcccggg cgctggccaa
     3901 gcaactggaa gccgacctgg ccatcatcga caagcggcgg ccgcgcgcca atgtgtccga
     3961 ggtcatgaac atcatcggcg aggtcgatgg ccgcacctgc atcatcatgg acgacatggt
     4021 cgatacggcc ggcacgcttt gcaaggcggc gcaagccctg aaagaccgcg gcgccgtgcg
     4081 cgtgttcgcg tattgcacac accccgtgct gtcgggcggc gcggtgcagc gcatcaccga
     4141 gtcggccctc gacgaactcg tcgtgaccga caccattccc ctgtccgagg atgggctggc
     4201 ttgcggcaag atccgtcagc tgtcctgcgc ctcgctgctc ggcgaaacca tgctgcgcat
     4261 ctcgaccgcc gagtccgtca gttcgctgtt cgtcgattga ttccgttttt cccgtgttgg
     4321 ctggtcgcgg ccggcgcggg gctgtgtgcg gtgcgcaagc gccgcgtttt ttgacagggc
     4381 gcaagcccca actggagttt tccatgaaat tcaatgccgt ttcgcgtagc gaacagggta
     4441 cgagtgcgag ccgccgcctg cgccacgccg gtcgcgttcc cgccattgtg tacggtggcc
     4501 aaaccggccc cgtcagcatc gagctcgacc acaacgagat ctaccacgcc ctgcgcaaag
     4561 agcagttcca tgcctccatt ctctcgatgg aactcaatgg caagtcggaa caggttctgt
     4621 tgcgcgctgt gcagtggcac ccctacaagc agcaggtcct gcacgtggac tttcagcgcg
     4681 tcgatgcgtc gcacgccatc aacaccaagg tgccgctgca cttcctcaac gccgaggtct
     4741 cgcccgccgt caagctcaac agcgccgtca tcagccatgt gctgactgaa atcgacatcg
     4801 tctgcctgcc tgccaacctg ccgcagttca tcgaagtcga tctgtcgcag atcctggctg
     4861 gcgccacgct gcacctgtcc gacgtcacat tgcccattgg cgtgacctac gccgcgcacg
     4921 gtggcgacgc caacccggtg ctggccgccg ccttggtcaa ggctggcaag gcggaagaag
     4981 aggaaacgcc tgcggcgccc gctggcgaaa cgcctgccgc cccggcggaa taatccgcga
     5041 cggcgcggca acatgcgcca cgcctcaccc aatctgcctg gcgtgccggg tagggtggga
     5101 acggcggaac aaggccccgg ccgggcttat cctggccggg gcctgttttt tggcaacggc
     5161 cgcgtggcgt acactctgtc gtcgatatcg cggttgcgcg cggaacgcgc tggttttttg
     5221 gttgtctctc catcatgtct gaacccatac gcttgatcat cggcctgggc aatcccgggc
     5281 ccgagtacga aacgacgcgg cacaacgccg gattctggtt ggccgaccat ttcgccgatg
     5341 atttgtcagg cgtgttcacg ttggaaaaag cgttctccgc ctgggtcgcc aaaggacggt
     5401 tcgagggtgc ggccatcgtg ttggccaagc cctccacgtt catgaaccgc tcgggcctgg
     5461 ctgtcggcac cctggcgcat ttctacaagc tcgcgcccga gcaggtgctg gtgctgcatg
     5521 atgaactcga ccttctgccc ggccaggtca agctcaagca aggaggcggg catgccgggc
     5581 acaacggctt gcgcgacatc cagcgcgttc tcgcgagccc ggacttctgg cgcctgcgcg
     5641 tggggatcgg gcatccgcgc acactggggc tgacccagca agtggccggg ttcgtgctga
     5701 accccccgaa gcgcgatgaa ctgcacaaga tcgaatcggt catcgaccgt tgccgggccg
     5761 ttctgcctgc gctgctgcgc ggggagtttt cgcaggcggt caaccagttg cacgagggca
     5821 atcgtgagtg acgaagcatg actgatgcag ttcgatcgct gggctttcgc gctgagctgc
     5881 gggattttct gcgattcgtg gcacggccga gcttcggtcc gcgcttgccc gggcgcgtag
     5941 ccatgcatgg gtgggtggag gactggtttc ccagcctgcg tccgggcagc ttgctgcgct
     6001 gggccgtcgt gctttggatc gtcaacctgt ttttcctggg cccgctttca cttgccgccg
     6061 ccatggcggg aggcgcccag agccgtctga atctttggaa catcccctgg gtggcggcgt
     6121 tgttgtgggc gcccgtcgtg gaggagctgg tatttcgcta tggcctgcgc cacattgccc
     6181 aggcgttgtg gctggtgccg gccgcgctgg tcgccatgtt gctgggtttg cgctggtatt
     6241 ccgtggtgtt gctggtggtg gtgctagccg tgtgctggat cccggtggcg ctgggctggc
     6301 gggtcgcgcg gcgacccctg ccatggcgct ggcgcagacg gtatcagcgc gcgtttccgt
     6361 gggtgttcca cgtctcgtgc ctgctgttcg ccggcgcgca tctggccaat ttttcgtatg
     6421 acagcacccc cctttgggtg atgccgtttc tggtgctgcc gcagtggctg accggcgtgg
     6481 tgctgggatg gcagcgcatg cgtcggggcg tgggggcgtc catgctgttg cacggcatct
     6541 tcaacgcggg accgcttggc atggtctggc tggtgctgca tctggcgccg catctgatga
     6601 cttgaggccg gatgtggcgg ggcccatggc gaacccgcca acggtactcg atggtcgctt
     6661 cattgcggct gcccgtacaa gggcccgggt tacaatcact tttttctcca ggaactcaga
     6721 acaatggcct tgcaatgcgg catcgttggc ctgcccaacg tcggaaaatc aacgctcttc
     6781 aatgccctga ccaaggccgg catcgcggcg gaaaactacc cgttctgcac catcgaaccc
     6841 aacgtgggcg tggtcgaagt gccggatgag cggctggccg cgctggccgg catcgtcaaa
     6901 cccgagcgca tcgtgcccgc cgtggtcgag ttcgtcgaca tcgccggcct ggtggctggc
     6961 gcctccaagg gcgagggcct gggcaaccag ttcctggcca acatccgcga aaccgacgcc
     7021 attgtgcatg tggtgcgctg cttcgacgac gacaacgtgg tgcacgtcgc cggcaaggtg
     7081 gaccccattt cagacatcgg ggtcatcaac accgaactgg cgctggccga cctcgccacg
     7141 gctgacaaag ccctgcagaa gaacctgaag ctttcgcgat cgggcgacaa ggaagccgcc
     7201 aagctggtgg ccttgctcga gaagatcgtg gccgcgttga acgaagcgcg cgccatccgg
     7261 tcgctgggcc tggacgagga ctcgctggcc atgattcggc agtactgctt catcacggcc
     7321 aagcccacct tgtatgtggc caacgtcaag gacgatggtt tccacgacaa ccccaaactg
     7381 caagccgtgc aggattacgc caagactgaa aacgcgcccg tcgtggcggt gtgcgctgcc
     7441 gtagaagccg agatcgccga cctggacgac gcggacaagc acgagttcct ggccgacatg
     7501 ggcatggatg aacctggcct caaccgcgtc atccgcgcgg gctatcggct gctggggctg
     7561 cacacctact tcactgctgg ccctaaggaa gtgcgcgcct ggaccatgca catcggcgac
     7621 accgctccca aggccgccgg cgtcatccac acggacttcg aacgcggctt catccgcgcc
     7681 cagaccatag gctacgacga ctacattgcc tgcaagggcg agcagggcgc gaaagaagcc
     7741 gggaagatgc gggcagaggg aaaggagtac gtggtgaagg atggggacgt gatgaatttc
     7801 ttgttcaacg tctgacgaat tttgctggat ctcggtggcg ctcagtagca cttggtgggc
     7861 gggtaggccc atttgaaatc aatgggttcc cggagatgaa gggtttttcg ccgcgcaacg
     7921 tcaagtacat gcgggcgttt gccgaggcct ggccggacgc cgaaattgtg cagcaggctg
     7981 ctgcacaatt gccctggggg cacaacctgg tattgctgga tcgcctgaaa gtgccaacgg
     8041 agcgcctggc ttatgcccgc gcggccatcg aacatggctg gtcgcgcaat gtgctgaaca
     8101 tccacatcga aatccgcctg ctggagcgca cgggcaaagc cgtcaacctc gccgccttgc
     8161 actggccgtc gtaaacatgc cgctgctgga tgtaggtctt gtacgccggg atcagcgagc
     8221 ccgacccggc cagccgatgt gcttcgtcca gcaccgcacg ctgctccggc tggcggtgat
     8281 atggcggatt tccagaaaca gggtaatcca acgattaaat gaggtaattt tcgcctccat
     8341 tgcccctttt tcaggtaaag atctcaggta gccatgcctc gccctgtagg tgggcccgac
     8401 tacaggcaat tgctgcgtcg tcgcagtagc cggcgatctt ttttataaaa actattacaa
     8461 tggctctaat cgtaattaaa aacatgtaaa agacattttt atggtctttt cactttccgt
     8521 tccgacagaa atcctgcaca cgctaggcgc ccgcttgcgg gcccagcgtc tcgctcaggc
     8581 gctgccgcaa cgcgatctcg cgcagatggc tggtttgtcc ctcggtgcgt tgcgcaagct
     8641 tgaacgcgat gggcagtgtt ccctggaaac cctggtcagg gtggttcagg ccttgggtct
     8701 ggcgcacgag ttggacgatc tcttcgtcct caaacgccaa tccatcgccc agatggaaca
     8761 ggcagaggac gtcagccagc gccaacgtgc cccgcgcagg aaatcgccat gaagcagttg
     8821 accgtgcatt actgcggctg gggtgaggat tggccgctgg ggcgcatggc tgacgatggt
     8881 caggcattgc tgttcgagta ttcccccgag gcactggcgc aggggttgga gctgtcgccc
     8941 ctgcatctca agctgcgcgc acaggcttac ggcggctttc cggcccactt gcaacggctg
     9001 cctggtttgg tcgccgacag tctgccagac ggctggggtt tgctgctgat ggatcgcctg
     9061 tttcgtcaac agggtgtgcg gcatcccggc ccactggacc gtctggcctt catcggcgat
     9121 cgtgccatgg gtgccctgcg ttttgtcccg tcccatgata ccgatgtcca ggaaccggac
     9181 tgggatctgc ttgtgctggc cgaggaaagc acccacgcgt tggccggtga ggcccgcgcc
     9241 gccttgcgtg agcttgcgct ggcgggtggg tcaccacaag gtgcccgtcc caaggctgtg
     9301 gtgcgatttg actcgaccac aggacgggtc agcacccggt cggacgcgcc cggtgagtcg
     9361 tggctggtga agtttcccgc tcagggtgaa cacaaggaag tctgcgccat cgagcagctc
     9421 tatgccgaat tggctcgcga ttgtggactg gaaatgccgg acagtcattg gttcgacctg
     9481 tcgcccagtc tggcagcgtt tggcgtggcg cgcttcgatc ggggtcaggg cttgcgcgtt
     9541 ccgttgcata gcctggccgg cctgctgcag gtggacttcc gtctgcccgg cgccaccgac
     9601 tacaccgctt tcctgcgcgc cacccgtctg atgacccatg atgagcgcga ggtcgagaag
     9661 gcctatgcgc gcgccgtttt caacgtgctc ttccataatc gagatgacca tccgaaaaat
     9721 tttgcgtggt gtctcggtcg tgaccggcgc tggcgcctcg cgccggcttt cgacctgacc
     9781 ttcagtgaag ggcccatggg gcagcaccac atggatgttt gcggggaggg ggccatggtt
     9841 gggcgcccac atctgctgcg cttggcagta gaaggcggtg tgcaactgaa agtcgcggaa
     9901 cggatcattg agcggcaatt ggagcaggcg tcggattttc cccggcgcat ctccgccttt
     9961 cctgtccgcc aggccacggc gcaggcgatg aactcggcga ttgaggcttg ccgcggaagg
    10021 ctggagagcc gctgacaaat cgcacgctgc cgaccaactg gacgtgctgg ccctgctgat
    10081 cgagcagtac gagcatgaac acttccc
//
LOCUS       DASTNK010000131         7694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1213414, whole genome shotgun sequence.
ACCESSION   DASTNK010000131 DASTNK010000000
VERSION     DASTNK010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7694
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213414"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..106
                     /locus_tag="VFR20_09845"
     CDS             <1..106
                     /locus_tag="VFR20_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009911514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0230862.1"
                     /translation="MTVLDYGKIIAEGLPADVRKNPKVIEAYLGTGGH"
     gene            110..826
                     /locus_tag="VFR20_09850"
     CDS             110..826
                     /locus_tag="VFR20_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0230863.1"
                     /translation="MSETLLKITGLQVNYGGIHAVKGVDFEVNTGELVTLIGANGAGK
                     STTMRAITGLKSYDQGDIQYMGQSIKGVPTHELLNRGLVMVPEGRGIFARMSIYENMM
                     MGAYKRNDKAGIEADIERMFTFFPRLKERATQLAGTLSGGEQQMLAMSRAVLSQPKLL
                     LLDEPSMGLSPILVDKIFEVVRDISQKGITVLLVEQNARLALQAANRGYVMDSGIITM
                     TGDAEEMLNDPRVRAAYLGE"
     gene            complement(1038..1122)
                     /locus_tag="VFR20_09855"
     tRNA            complement(1038..1122)
                     /locus_tag="VFR20_09855"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1086..1088),aa:Leu,seq:tag)
     gene            1278..2465
                     /gene="dapC"
                     /locus_tag="VFR20_09860"
     CDS             1278..2465
                     /gene="dapC"
                     /locus_tag="VFR20_09860"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEU0230864.1"
                     /translation="MNPRLGALHPYPFERLRLLLAGANTPPTRLDPINLSIGEPKHKT
                     PDVLVRAMRGAMDGLAKYPPTKGDATLRGAIANWIERRYGIPRPDPDTHVLPALGSRE
                     ALFACAQAVIDPTADAYVVCPNPFYQIYEGAALLAGARPWFVNARADLAFGCDWNSVP
                     ADVWNKTQLVFVCSPGNPAGNVMGLDEWKTLFALSDQYGFVIASDECYSEIYFDENQK
                     PLGGMQAAHQLGRGDFHNLLCFSSLSKRSNAPGLRSGFIAGDAALIAQCLRYRTYHGS
                     AMSAVVAQASVAAWNDEHHVLENRQRYREKFEAVVPLLAPVLNVEWPQASFYLWAGTR
                     GSDTDFARDLYGATAVTVLPGSYLAREAHGVNPGVGRVRIALVANIDECIEAAGRIAA
                     FIR"
     gene            2546..3367
                     /gene="dapD"
                     /locus_tag="VFR20_09865"
     CDS             2546..3367
                     /gene="dapD"
                     /locus_tag="VFR20_09865"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEU0230865.1"
                     /translation="MTSDLQITIEQAWENRAQITPGPEHATVREAVLTTLEGLDTGRL
                     RVAEKIDAGWVVHQWIKKAVLLSFRLENNGVMGQAPLQYYDKVPVKFADYDDAKFAAG
                     GFRVVPPATARRGAFIARNVVLMPSYVNVGAYVDEGTMVDTWATVGSCAQIGKNVHLS
                     GGVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEENSVLAMGVFISQSTKIYDR
                     ATGSITYGRIPSGSVVVPGSLPAADGSHSLACAVIVKRVDAQTRAKTSVNDLLRT"
     gene            3367..4518
                     /gene="dapE"
                     /locus_tag="VFR20_09870"
     CDS             3367..4518
                     /gene="dapE"
                     /locus_tag="VFR20_09870"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEU0230866.1"
                     /translation="MSDSAVLDLAKRLIARPSVTPADEGCQALLADRLRPLGFACETL
                     AFGEVVNLWARRGTEAPLFVFAGHTDVVPTGPLEKWHGDPFVPVERDGRLYGRGAADM
                     KSSIAAFVVAAEEFVAAHPDHNGSIALLLTSDEEGPATHGTAKVCDVLVQRGEHLDYC
                     VVGEPTSVNTLGDTIKNGRRGSLSGRLIVKGLQGHVAYPHLARNPIHELAPALAELTQ
                     TQWDSGSDYFPATTFQVSNLHAGTGATNVIPGQAVVDFNFRFSTSSSPESLQERVEGV
                     LHKHRLDFDLAWTLGGEPFLTPRGALSRAVTQAIQAETGVATELSTTGGTSDGRFIAR
                     ICPQVVELGPENATIHQIDENIPLASLDPLKNIYRRTLELLLLNEDAHA"
     gene            4511..5404
                     /gene="prmB"
                     /locus_tag="VFR20_09875"
     CDS             4511..5404
                     /gene="prmB"
                     /locus_tag="VFR20_09875"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU0230867.1"
                     /translation="MPDTCETLLTLRDLLRYTVSRFNAAKLSFGHGNDNAWDEAVYLL
                     LHTLHLPLDTLDPFLDARVLPEERERFLALVERRLAERLPAAYLTGEAWLQGYRFTVD
                     PSVIIPRSPIAECLVQGLAPWVTQPDDVDYALDLCTGSGCLAILAALAFENAQIDAVD
                     ISENALAIADTNIEQFGLDGRVTTYRSDIFDQLPHCEYKLILCNPPYVNTRSMAQLPA
                     EYRHEPTMALAGGDDGMDLVRRILREAPGFMAPEGLLMLEIGNEYDHFVAAFPELDPV
                     WISTENTEDQILLLTREQLVS"
     gene            5401..7341
                     /locus_tag="VFR20_09880"
     CDS             5401..7341
                     /locus_tag="VFR20_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0230868.1"
                     /translation="MIRATNLTLRRGTKVLLEDAGFVINPGERVGIVGKNGAGKSSLF
                     ALMQGGLDPDAGMLSIPIGWRIAGVDQQIPALDRPAREFVIDGDTHLRALQTERAHTP
                     DSQGSRIAELEAELVEAGAWSAASRAEQLLAGLGFTPDQWMEPVRTFSGGWRMRLALA
                     RALMAPSELLLLDEPTNHLDLDAMIWLERWLAAYTGTVLVISHDTEFLDAVAKVILHF
                     DHASIVRYRGGYQDFLTQRAERTRQSQIAWEKQTRDAARLQHFIDRFKAKATKAKQAQ
                     SRVKALARMQVLTPLRAEAGVDIHLPAPESMPDPLLTLEGIAAGYQAPDHTDKIILRD
                     ITLMVRGGARTGVLGVNGAGKSTLIKTLAGEQSALAGTRTASKGLKIGYFAQHQLDVL
                     DPGGTPLAHLTRLAPDTREQELRNYLGGFGFGGDAVTGLVAPMSGGEKARLALSLIVW
                     QKPNMLLLDEPSNHLDVDTREALAEALAEFEGSVLLVSHDRHLLRTTVDDFWIVADGR
                     VQEFDGDLDDYRDWLSQRNADARAQARQVTQATNTEGGGDRKADRRQRAQARQRLAQQ
                     RKPLTDKIKVIEHQLEGLRQRLSELDTLIADPDLYSDARRDERLRVLAEHGEKVKTVE
                     DFEGRWFELQEALEKQEDKDGD"
     gene            complement(7410..>7694)
                     /locus_tag="VFR20_09885"
     CDS             complement(7410..>7694)
                     /locus_tag="VFR20_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEU0230869.1"
                     /translation="GKAFIVGHITVKDAEKWAEYCRRVPGTVAPWGGEVLVRGTRTSV
                     LAGEHDYTDTVVIRFPDMRSVNAWHDSPAYQELAKLRRQAADVVIISYEG"
BASE COUNT         1515 a         2499 c         2321 g         1359 t
ORIGIN      
        1 catgaccgtg ctggattacg gcaaaatcat cgctgaagga ctacccgcgg atgtccggaa
       61 gaatccgaaa gtcatcgaag cctatctggg cacgggaggt cactaagcca tgtccgaaac
      121 gctactcaaa atcacggggc tgcaggtcaa ctatggcggc atacacgccg tcaaaggtgt
      181 ggacttcgaa gtgaatacgg gtgaattggt gacactgatc ggcgccaacg gcgccggcaa
      241 gagcaccacc atgcgtgcca tcaccggcct gaaatcctat gaccagggcg acatccagta
      301 catgggtcag tcgatcaagg gcgtgcccac gcacgagttg ctgaaccggg gcctggtcat
      361 ggtgccggaa ggccgtggca tttttgcgcg catgtccatt tacgaaaaca tgatgatggg
      421 ggcctacaag cgcaacgaca aagccggcat cgaggcagac atcgagcgca tgttcacctt
      481 cttcccgcga ttgaaggaac gcgccaccca actggccggc acgctttcgg gcggcgaaca
      541 gcagatgctg gccatgtcgc gcgcggtgct gagccagccc aagctgctgc tgctcgacga
      601 accgtcaatg ggtctgtctc ccatcctggt cgacaagatc ttcgaggtcg tgcgcgacat
      661 ctcccagaag ggcatcaccg tactgctggt cgaacagaac gcccgcctgg ccctgcaagc
      721 cgccaaccgc ggctacgtga tggactccgg catcatcacc atgacgggcg acgccgagga
      781 aatgctgaac gacccgcgcg tgcgggcggc gtatctgggc gaatagcctg gcccgggcac
      841 cggccttcca aaccggcaaa aaaggcaact aagccacggc ttagttgcct tttttatttg
      901 gttttgccgc cgagaaccag agggtttatg caccacccat ttgtggcaac cccggcccca
      961 acccattggc tccacccatt gatagcccat gtttttattg atagcccatg ttttttattg
     1021 tgaatcaagg catcaagtgg tgcgaacgga gagactcgaa ctctcacacc tttcggcgcc
     1081 agaacctaaa tctggtgcgt ctaccaattc cgccacgttc gcaaaaggca agctgataag
     1141 tttagcttta ttgggcagcg cacgcaattt cccgggactc tccaaccatc tccggtcggc
     1201 aggccccggg aaatacgggc ctcccccgct cttgctaaaa tcgcccgttg gacaccggct
     1261 tctgtcatcg gacacccatg aaccccagac ttggcgcgtt gcacccctat cctttcgaac
     1321 gactgcgact gttgttggcc ggcgccaaca caccgcccac gcgacttgac cccatcaacc
     1381 tgtccatcgg cgaacccaag cataagacac ccgatgtgct ggtgcgtgcc atgcgcggcg
     1441 ccatggacgg tctcgccaaa tacccgccca cgaaaggcga cgcgaccctg cgtggcgcca
     1501 tcgccaactg gatcgagcgt cgctacggca ttccccgccc cgaccccgac acccacgtgt
     1561 tgccggcgct gggttcgcgc gaagcgctgt tcgcctgcgc gcaggctgtc atcgacccaa
     1621 ccgccgatgc ctatgtggtg tgcccgaatc cgttctacca gatctacgaa ggcgcagccc
     1681 tgcttgccgg cgcccgcccg tggttcgtca acgcgcgcgc cgatttggca ttcggttgcg
     1741 actggaacag cgtgcccgcc gatgtctgga acaagacgca actggtgttt gtctgctcgc
     1801 ccggcaatcc cgccggcaac gtcatgggcc tggacgagtg gaagacgctg ttcgcgctgt
     1861 ccgatcagta cgggtttgtg atcgcgtcgg acgagtgcta ttcagaaatc tacttcgacg
     1921 aaaaccagaa acccctgggc ggaatgcagg cggcgcatca actgggccgt ggcgacttcc
     1981 acaacctgtt gtgtttctca agcctgtcga agcgctccaa cgccccgggt ctgcgctcgg
     2041 gtttcatcgc gggtgacgcg gcgctcatcg cccaatgctt gcgctatcgc acctatcatg
     2101 gcagcgccat gagcgcggtg gtggcgcaag cgagcgtggc ggcctggaac gatgagcatc
     2161 atgttctgga aaaccggcag cgctatcgcg aaaaattcga ggcggtggta cccttgctgg
     2221 cccccgtcct gaatgtggaa tggccacaag cttcttttta cttatgggcg ggtacccggg
     2281 gttcagacac ggatttcgca cgcgacctgt acggcgccac cgcggtcacc gttctgccag
     2341 gaagctactt ggcgcgcgag gcgcacggcg tgaaccctgg cgtcggacgc gtgcgcatcg
     2401 ccctggtggc gaacatcgat gaatgcatcg aggcggcggg gaggatcgcg gctttcatcc
     2461 gctgaccgac cacaaggtca tccctagagg cgatacgccc ccgtccgcct cactttccaa
     2521 gacgattcac tacttcttca agcccatgac atcagatctg caaataacca tagaacaagc
     2581 ctgggaaaat cgtgcccaaa tcacgccggg ccccgaacac gccaccgttc gcgaggcggt
     2641 gctcaccacg ctcgaagggc tggacaccgg gcgcctgcgc gtggctgaga aaatcgatgc
     2701 cggctgggtg gtgcaccagt ggatcaagaa agccgtgctg ctgtctttcc gtctggaaaa
     2761 caatggcgtc atgggccagg cgccactgca atactacgac aaggtgccag tgaaattcgc
     2821 tgattatgac gacgccaagt tcgccgccgg cggctttcgc gtggtgccgc cggccacggc
     2881 ccgtcgcggc gccttcatcg cacgcaatgt cgtgctcatg ccgtcgtacg tgaacgtggg
     2941 tgcctatgtc gatgaaggca ccatggtcga cacctgggcc accgtggggt cttgcgccca
     3001 gatcggcaag aacgtgcacc tgtcgggtgg cgtgggtatt ggcggtgtgc tggaaccgct
     3061 gcaggccaac cccaccatca tcgaagacaa ctgcttcatc ggcgcccgct ctgaagtggt
     3121 cgaaggcgtc atcgtggaag aaaactcggt gctggccatg ggtgtgttca tttcgcaaag
     3181 caccaagatc tacgatcgcg ccaccggatc gatcacctat ggccgcatcc cttccgggtc
     3241 ggttgtcgtg cccgggtcgc tgcccgccgc cgatggttcc cacagcctgg cctgcgcagt
     3301 catcgtcaag cgggtcgatg cccagacgcg cgccaagacc agcgtcaacg acttgctgag
     3361 gacctgatgt ccgactccgc cgtacttgac ctggccaagc gcctcatcgc acgcccatcg
     3421 gtcacacctg ctgacgaagg gtgccaggct ctgctcgccg accgtctgcg cccgttggga
     3481 ttcgcctgcg agaccctggc tttcggcgag gtcgtcaatc tatgggcgcg gcgcggcacg
     3541 gaagcaccgt tgttcgtctt cgccggacac accgacgtcg tgcccactgg tccgctggaa
     3601 aaatggcacg gggatccgtt cgttcccgtg gaacgcgacg ggcggctcta cgggcgtggc
     3661 gccgccgaca tgaaaagctc gatcgccgcc ttcgtcgtgg ccgccgaaga attcgtcgcc
     3721 gcccatccgg accacaacgg ctccatcgcg ctgcttctga cctccgatga agagggtccc
     3781 gccacgcacg gcacggccaa agtctgcgac gttctggtcc aacgcggcga acacctcgac
     3841 tactgcgtcg tgggcgaacc cacctcggtg aacacgctgg gcgacaccat caagaacggg
     3901 cgccgcggtt cactgtcggg gcggctgatc gtcaagggtt tgcaaggcca cgtcgcctac
     3961 ccgcatctgg cgcgcaatcc catccatgag ctcgcccccg ccctggccga actgacgcag
     4021 actcaatggg atagcggcag cgattatttc ccggccacga ccttccaggt gtcgaatctg
     4081 cacgccggaa ccggcgcgac caatgtgata ccgggccagg cggtggtgga cttcaatttc
     4141 cgcttctcca cgtccagcag ccccgaatcg ctacaggaac gcgtcgaggg cgtgctgcac
     4201 aagcatcgcc tggacttcga cctggcctgg acgctgggcg gtgaaccgtt cctgaccccg
     4261 cgtggcgcac tgagccgagc ggtgacacag gccatccagg ccgaaaccgg ggtggccacc
     4321 gaactgtcga ccaccggcgg cacttccgat gggcggttca tcgccaggat ctgcccgcag
     4381 gttgtggagc tcggacccga gaacgccacc atccatcaaa tcgacgagaa cattcccttg
     4441 gcgtcgctgg atccgctgaa gaacatctac cggcgcacgc tggaactact cctactgaac
     4501 gaggacgcgc atgcctgaca cctgcgaaac attgctgaca ctgcgggatc tgctgcgcta
     4561 cacggtgtcc cgtttcaacg cggccaaact gtcgttcggc catggcaatg acaatgcctg
     4621 ggacgaggcg gtgtacctgc tgctgcacac gctgcatctg ccgctggaca cgctggaccc
     4681 tttcctcgac gcgcgcgtgc tgcccgagga acgcgaacgg ttcctggcgc tggtcgaacg
     4741 ccgactcgcg gagcgcctac cagcggccta cctgaccggc gaggcttggc tgcaggggta
     4801 ccggttcacc gttgacccaa gcgtcatcat tccgcgctca cccatcgccg aatgcctggt
     4861 gcagggcctg gcgccttggg tcacccaacc cgacgacgtc gattacgcgc tcgatctgtg
     4921 caccggatcc ggctgcctgg ccatcctggc cgcactggca ttcgagaatg cacagatcga
     4981 cgcggtcgac atttcggaaa atgcactggc gatcgccgac acgaacatcg agcaattcgg
     5041 tctggacggc cgtgtgacca cttaccgcag cgacatcttc gaccaattgc cgcattgcga
     5101 gtacaagctg atcctctgta atccgcctta cgtcaacacg cgctccatgg cacagttgcc
     5161 ggccgaatac cgtcacgagc ccaccatggc gctggctggc ggggacgacg gcatggatct
     5221 cgtgcgccgc atcctgcgcg aagccccggg gttcatggcg cccgaaggcc tgctgatgct
     5281 ggaaatcggc aacgaatatg atcatttcgt cgcggcgttc ccagagctcg atcccgtgtg
     5341 gatcagcacg gaaaacactg aagaccagat cctgctgctg acgcgggaac aattggtctc
     5401 gtgatacgcg ccacaaacct cactctgcgt cgcggcacca aggtcctgct cgaagacgcg
     5461 gggtttgtca tcaaccctgg cgaacgggtt ggtatcgtgg ggaagaacgg cgccggcaaa
     5521 tccagcctgt ttgccctgat gcagggcggg ctcgacccgg atgcgggcat gctgtcgatt
     5581 ccaatcggct ggcgcatcgc cggcgtcgat cagcagatcc ccgcgctgga tcgccccgcg
     5641 cgcgagttcg tgatagacgg tgacacacac ctgcgcgcgc ttcaaaccga acgcgcccat
     5701 acccccgaca gccagggtag ccgcatcgcg gaactcgaag ccgaactggt cgaagccggc
     5761 gcctggagcg ccgcgtcgcg cgccgaacag ttgttggcag gtctgggctt cacccctgac
     5821 caatggatgg agccggtgcg caccttctcg ggcgggtggc gcatgcggtt ggccctggcc
     5881 cgcgccctga tggcgccctc ggaactgctg ctgctcgatg aacccaccaa ccacctggat
     5941 ctggacgcca tgatctggct ggagcgctgg ctggccgcct acacgggcac ggtattggtg
     6001 atttcgcacg acacagaatt cctggacgcg gtggccaagg tcatcctgca cttcgatcac
     6061 gcaagcatcg tgcgctaccg tggcggctac caggatttcc tgacccaacg cgccgaacgc
     6121 acccgacaga gtcagatcgc gtgggaaaaa cagacacgcg acgcggcacg cctgcaacac
     6181 ttcatcgatc ggttcaaggc caaggccacg aaagccaagc aggcacaaag ccgtgtgaaa
     6241 gcgttggcgc gcatgcaggt tctgacccca ttgcgcgccg aggcgggtgt ggatatccat
     6301 ctgccggccc ccgagagcat gcccgatccg ctgttgacgc tggagggcat cgcggccggc
     6361 taccaagccc cggaccatac agacaagatc atcctgcgcg acatcacgct gatggtccgc
     6421 ggcggcgcgc gcaccggcgt gctgggcgtc aatggcgcgg ggaaaagcac gctcatcaag
     6481 acgctggcgg gcgaacagag cgcgttggcg ggaacacgca cggcatcgaa aggtttgaag
     6541 atcggctact tcgcccagca tcaactcgat gtactggatc ccggtggcac gccgcttgcg
     6601 catctgactc ggctcgcccc cgatacacgc gaacaggaac tgagaaacta tctgggcggg
     6661 ttcggtttcg gcggcgacgc ggtcaccggc ttggtggcgc ccatgtcagg cggcgagaag
     6721 gcgcgcctgg ctctgtccct catcgtctgg cagaagccaa acatgctgct gctggacgaa
     6781 cccagcaacc acctggacgt ggatacgcgt gaagccctgg cggaagcctt ggccgaattc
     6841 gaggggtcgg tgctgctcgt gtcgcacgac cgtcacctgc tgcgcaccac cgttgacgat
     6901 ttctggatcg tggccgatgg ccgggttcaa gagttcgacg gcgatctgga tgattaccgt
     6961 gactggctct cgcagcggaa cgccgacgcg cgcgcgcagg cacggcaagt cacacaagcc
     7021 acgaacacgg agggtggggg cgatcgcaag gccgacagac ggcagcgggc gcaggcacgc
     7081 caacgcctgg cccaacagcg caagccgctg actgacaaga tcaaagtgat cgaacaccag
     7141 ttggaaggcc tgcgtcagcg gctctctgaa ctcgatacgc tgatcgcgga tccggatctc
     7201 tattccgatg cgcgacgcga cgaacgcttg cgcgtattgg ccgagcatgg ggagaaggtc
     7261 aagaccgtcg aagacttcga aggccggtgg ttcgaactgc aggaagcgct ggaaaaacag
     7321 gaagacaaag acggggattg agacggtcac cgcgacggtg ctcaactccc ctcagcccgc
     7381 gccaccatgg aataccccgc ctcgtttccc tacccttcgt agctgatgat cacgacgtcg
     7441 gcggcctgcc ggcgcagctt cgccagttcc tggtaagccg gtgaatcatg ccaggcgttg
     7501 acggacctca tatcgggaaa gcgaatgacg accgtgtcgg tgtagtcgtg ctcacccgcg
     7561 agcaccgagg tgcgggtgcc gcgcaccagc acctcgccgc cccacggcgc cacggtgccg
     7621 ggcactcggc gacaatactc agcccacttc tccgcatcct tgaccgtgat gtggcccacg
     7681 atgaaagcct tgcc
//
LOCUS       DASTNK010000132         5844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1584536, whole genome shotgun sequence.
ACCESSION   DASTNK010000132 DASTNK010000000
VERSION     DASTNK010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584536"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..941)
                     /locus_tag="VFR20_09890"
     CDS             complement(147..941)
                     /locus_tag="VFR20_09890"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0230870.1"
                     /translation="MMKNGPLALVFHSLVVIFILAPLVIVCWVAFTPAPTLTLPFTEF
                     SLRWFRAIFDHSDFVQAFKISLWLAFFSATLAVCLGAPAALAIARHQFRGRDFLNGLF
                     LSPLMIPHLVLGISMLRFFALLNQAGSFYWLVLGHVVVITPYVMRLVLGALVGFDRSI
                     EHAAESLGASRAEVFFKITLPLILPGLAGGWLLAFINSFDELNMSIFITSPSTVTLPV
                     RMYMYATESIDPMMAAVSAVVIGLTAVAMFVLDRVYGLDKVLVGRH"
     gene            complement(948..1799)
                     /locus_tag="VFR20_09895"
     CDS             complement(948..1799)
                     /locus_tag="VFR20_09895"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0230871.1"
                     /translation="MATTRRGIGAWWGTMPLGVLFTLLVLIPLVLTLVLSFHQFDYQK
                     GILPGYTTGQYLTVVTDSYFLKIFWRTLWISLVTTALCVVIGGPEAYILSRMGRPWRS
                     IFLLLVLSPLLVSLVVRAFGWTILLNPAGIVGQIATRLGFGPIMYTPTAVIIALVHVM
                     LPFMVIPVWTSLQKADPTVEQAAYSLNATRSQTFWHVVAPQALPGLLSGSLIVFGLTA
                     SSFAIPALLGGRRLKLAASVVYDQFLVQLNWPLGAAIAILLLIANVLIMMSYGRIVER
                     FYKRSLG"
     gene            complement(1804..2856)
                     /locus_tag="VFR20_09900"
     CDS             complement(1804..2856)
                     /locus_tag="VFR20_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0230872.1"
                     /translation="MSFLTLTGVSKNYGALRVVSDLSLSVEKGEFVSLLGPSGCGKTT
                     TLQMIAGFADVTQGTISLDGVDITHARPNTRGLGIVFQTYALFPHMTVGRNVAFGLAT
                     RKVPKAEQAERVAQALSLVKLDKYADRYPRELSGGQRQRVALARALVIRPPVLLLDEP
                     LSNLDAKLREEMQYELRRIQRTVGTTTIMVTHDQSEAMSTSDRVVVMQEGRAVQVDEP
                     YRIYEHPRTSFISTFVGKTNLVDGEVATIDGRQVVRCGPLTLDAIDLPSAGSRVRVSL
                     RPEKLFLTDQVGRLSGCVVRSRYFLGSQWLYELDSVLGPLVVVTPNDGKRVYDANANV
                     NVDWVSDAVRTLPGEH"
     gene            3056..3937
                     /locus_tag="VFR20_09905"
     CDS             3056..3937
                     /locus_tag="VFR20_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU0230873.1"
                     /translation="MKNQEDDTSSETSGTLQRGFAVIRALAEVPDGGLRLKQVAQATG
                     LAQPTAHRLLKSLVQERVVEQTAGGKNYRLCIEFYALAARAGSGYSSFRELCRPALLR
                     LSGVLNDTVFLMVRSGFDVLCMDRVEGPLPIRSYTSDIGGRIPLGLGQAGTVILALLP
                     PGEQEEIIRFNVPRLRHLGYIDEVFLRAQIERARAQNHAVSQGQGPIQGMGGVSVAIT
                     DPNGYVVGAISIGTLSERLNDERLPHILHVLHQEADRISRQVNPFDPALKHPSQYLGP
                     AHVGDVKPADFSITPPL"
     gene            complement(3994..5250)
                     /locus_tag="VFR20_09910"
     CDS             complement(3994..5250)
                     /locus_tag="VFR20_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HEU0230874.1"
                     /translation="MATLSQTPPSAPALSPARAYTTLFVSTFAFLVCFVVWMMFGVLG
                     IPIRHELGLNSTEFGLLTATPVLTGAIMRLPLGAWTDRFGGRNMMTALLVACAPPVYL
                     VSYATQLWQFLLIGLCLGCVGASFAIGTPYVARFFSQEKRGFVMGFFGAGAVGAAVNL
                     FITPGLQAAYGWRMVPRVYAVVLLVTAVVFWLLAARDPGKGRAGAPMRDAFKVLRDRR
                     VWHLCQYYSITFGGFTALSLWLPQYLTLEYGMSVAMAAALAAGFALPGSILRAVGGAL
                     SDRFGAHAVTWWGLWVAWVCLFILSYPKTDLVIHTVDGPVTLHLSISMVAFVVLAFIL
                     GAVLAFGMASTFKYVADDFQENMGLVTGIVGLAGGVGGFLLPILFGVLLDTFRIRSSC
                     FMLLYGIVWVSLILIYLSSVRKVRLV"
     gene            complement(5308..>5844)
                     /locus_tag="VFR20_09915"
     CDS             complement(5308..>5844)
                     /locus_tag="VFR20_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0230875.1"
                     /translation="CCVAMAMPQVQIVAYCGDLGYGTANGANMLSLMLSMGIISRLGS
                     GWLSDRIGGLRTLLLGSLLQGLALWLYVFNHGLVSLYVVSALFGLFQGGIVPSYAVIV
                     REHFPPEVAGARVGTALMATLVGMALGGWMSGAIYDLTYSYHAAFLNGIAWNALNAAI
                     VIFLLYRSRPGRLVRQAV"
BASE COUNT         1343 a         1904 c         1684 g          913 t
ORIGIN      
        1 cagcgcgctg tgcgcctcac catcgagaaa atagtcgcaa cgcggtcgat cgcattcagc
       61 cacgcgccat agcaacgcct tggattcact gcccattcgc ttctctttac ttgtttccat
      121 gccatgcgct caaggcgcca cccgccctaa tgacgcccca ccagaacttt gtccagccca
      181 tacacgcggt cgagaacgaa catggccacg gccgtcaatc cgatcaccac cgccgagacc
      241 gccgccatca tgggatcgat cgattcggtg gcatacatgt acatgcgcac cggcagcgtc
      301 accgtggagg gcgaagtaat gaatatcgac atgttcagtt catcgaaact gttgatgaag
      361 gccagcagcc agccacccgc caggccaggc aggatcaacg gcagtgtgat cttgaagaac
      421 acctcggcac ggctcgcacc cagcgactcg gcggcgtgct cgatgctgcg atcgaatccg
      481 accaatgccc ccagcaccag gcgcatcaca tagggggtga tcaccacgac atggcccagc
      541 accagccaat agaacgaccc cgcctgattc aacaaggcga agaagcgcag catggagatg
      601 cccagcacca gatggggaat catcagcggc gaaagaaaca ggccgttgag gaaatccctt
      661 ccacggaatt ggtggcgcgc gatggccagc gcggccggtg cgcccaggca caccgccagg
      721 gtggccgaaa aaaacgccag ccacaggctg atcttgaagg cctgcacaaa atcactgtga
      781 tcgaaaatgg cgcggaacca ccgaagcgag aattcggtga acggcagcgt caatgtgggg
      841 gccggcgtga acgccaccca gcacacgatc accagcggcg ccaaaatgaa gatcaccacc
      901 agggagtgga aaaccagggc cagcgggccg ttcttcatca tggccttcta ccccaggctg
      961 cgcttgtaaa agcgctccac gatgcgcccg tacgacatca tgatcagtac gttcgcgatc
     1021 agcaaaagaa tggcgatagc cgcccccaat ggccagttca attggacaag gaactggtca
     1081 tagacaaccg atgccgcaag cttgaggcgc cggcctccca acagcgccgg gatggcgaac
     1141 gagctggcgg tcaggccgaa cacgatcagg ctcccggaca gcaacccagg cagcgcctgc
     1201 ggcgcgacga catgccagaa cgtctgcgat cgtgtggcgt tgagcgagta ggcggcctgc
     1261 tccacggttg gatcggcttt ctgcagcgag gtccacacag gaatgaccat gaacggcagc
     1321 atcacatgca ccagggcgat gatgaccgct gtcggggtgt acatgatcgg gccgaacccc
     1381 agccgggtgg cgatctggcc gacgatgccc gccgggttga gcagaatcgt ccagccgaaa
     1441 gcgcgcacca ccagggacac cagcaatggc gacagcacca gcagcaggaa aatagagcgc
     1501 cacggccgtc ccatgcgact gaggatatac gcctcgggcc cgccaatgac cacacagagc
     1561 gccgtggtca ccaatgagat ccacagcgtg cgccagaaaa tcttcaggaa gtagctgtcg
     1621 gtcacgacag tcaggtattg gcccgtcgtg tagccgggca gtatgccctt ctggtagtcg
     1681 aactgatgga acgacagaac cagcgtcaac accagcggaa tcagcaccag aagcgtgaac
     1741 agcacaccca atggcatcgt cccccaccac gcaccgatgc cccgccttgt cgttgccatg
     1801 gcttcagtgc tcgccaggaa gggtacgcac ggcatccgat acccaatcaa cgttgacgtt
     1861 ggcattcgcg tcgtagacac gcttgccgtc attgggcgtc accaccacca agggtccgag
     1921 caccgaatcc agctcgtaca gccactggct cccgaggaaa tagcgtgaac gcaccacaca
     1981 tcccgacagc cgccccacct ggtctgtcag gaacaatttc tccggacgca gggaaacccg
     2041 cacgcgcgat ccggcggatg ggagatcgat cgcatcgagg gtgagcggcc cgcatcggac
     2101 gacttggcgc ccatcaatag tggcgacctc gccatcgacc aggttggtct ttcccacgaa
     2161 cgtcgagatg aagctggtgc ggggatgctc ataaatacgg tagggctcat caacctgcac
     2221 cgcccgccct tcctgcatga ccaccacgcg atcgctggtg gacatggctt cggattgatc
     2281 atgcgtgacc atgatggtgg tggtgccaac cgtgcgttgg atgcgacgca gttcgtactg
     2341 catttcctca cgcagcttgg catccagatt ggacagcggt tcgtccagaa gcagcaccgg
     2401 cgggcggatg accagagcac gggccagggc cacgcgctgg cgctggccac cggacagctc
     2461 gcgcggatag cggtcggcgt acttgtcgag tttgaccagg ctcaatgcct gggccacgcg
     2521 ttcggcctgt tcggctttgg gcactttgcg cgtggccaat ccaaacgcca cattgcgccc
     2581 gacagtcatg tgggggaaca gcgcatacgt ctggaacacg atgccaagtc cgcgcgtgtt
     2641 cggcctcgca tgggtgatgt ccaccccatc gagtgagatg gtcccctgcg tcacgtcggc
     2701 gaatccggcg atcatttgca gcgtcgtggt cttgccacaa cccgaaggcc ccagaagcga
     2761 aacgaactcc cccttctcca cgctcaggct cagatcggag acgacccgaa gggccccgta
     2821 gttttttgaa actcccgtga gtgtcaagaa tgacatgttt gtcccttgcg gcgcgtgctt
     2881 gtcccggtct cccggcacgc catgccgact ggccgacgtg cttcttcaat gtgtcggcca
     2941 gtctaggaag caaaatccac tccgtcaacg ttttttcact attattggaa tttaattatt
     3001 taaattttca ttgaatgaaa ttaaaatatc ataattaata tcaatacaca taaaaatgaa
     3061 aaatcaagaa gatgatacat cttcggagac atccggcacg ctgcagcgcg gcttcgccgt
     3121 gatccgcgcc ctggccgagg ttcccgatgg cggtttgcgc ctcaaacaag tcgcccaggc
     3181 cactggcctg gcgcaaccca ccgcgcatcg attgttgaaa tccctggtcc aggaacgcgt
     3241 ggtcgaacaa accgcgggag ggaagaatta ccgactttgc atagagttct acgccctggc
     3301 ggccagggcc ggcagtgggt actcgtcgtt tcgcgagcta tgccgtccgg cgctgctgcg
     3361 cctgtcgggt gtgctgaatg acacggtctt cctcatggtg cgcagcggct tcgatgtcct
     3421 gtgcatggat cgcgtcgaag gtccgctgcc catccgctca tacaccagtg acataggcgg
     3481 ccgtattccc ctgggactcg gtcaggcggg caccgtgatc ctggccctgc taccgcccgg
     3541 cgagcaagaa gagatcatcc ggttcaatgt gcccaggctg cggcatctgg gctatatcga
     3601 cgaagtattc ctgcgcgccc agatcgagcg cgcacgcgca cagaaccacg ctgtcagcca
     3661 ggggcagggc cccatccagg gtatgggcgg ggtatccgta gccatcactg acccaaacgg
     3721 ctatgtggtg ggagcgatca gcatcggcac actcagtgaa cgcctgaatg acgaacgcct
     3781 gccgcacatc ctgcatgtcc tgcaccagga agccgaccga atcagccggc aagtcaaccc
     3841 tttcgacccc gccctcaagc atccctcgca gtacctgggg ccggcgcatg taggcgacgt
     3901 gaaaccggcg gatttctcga tcaccccgcc tctgtgatca agggtctgca tgctcggcca
     3961 gcggcgtcac cgccacccac aacccgccac ccgtcaaacc agccgcacct tccgcacgct
     4021 cgacagatag atcagaatca acgacaccca cacgatccca tacagcagca tgaaacagct
     4081 ggagcgaatg cggaaggtgt cgagcagcac gccaaaaaga atcggcagca ggaagccgcc
     4141 caccccgcca gccagcccca cgatccccgt gaccaatccc atgttttcct ggaaatcgtc
     4201 ggcgacatac ttgaacgtgg acgccatgcc gaacgccagc accgcgccca ggatgaaggc
     4261 cagcacgacg aacgccacca tagaaatgct cagatgcagt gtcaccgggc cgtcaaccgt
     4321 gtggatgacc aggtcggtct tcggatacga caggatgaac aggcagaccc acgccaccca
     4381 tagcccccac cacgtcaccg catgggcgcc gaagcgatcg gacaaggcgc cacccacggc
     4441 gcgcaggatc gacccgggca acgcgaaacc cgcggccaat gccgcggcca tggcgacgga
     4501 catgccatac tccaacgtca ggtactgtgg caaccacaac gacagggccg tgaatccgcc
     4561 gaacgtgatc gagtagtatt ggcacagatg ccagacccga cggtcgcgca gcaccttgaa
     4621 cgcgtcccgc attggcgcgc cggcccgccc cttaccggga tcgcgcgcgg ccagaagcca
     4681 gaaaaccacg gcggtgacga gcaacaccac ggcatacacg cgcggcacca tccgccatcc
     4741 ataagcggct tgcaggccgg gggtgatgaa cagattgacc gcggccccga ccgcgcccgc
     4801 accgaagaaa cccatgacga agccgcgctt ttcctgggag aagaaccgcg ccacatacgg
     4861 cgtgccgatg gcgaacgatg cccccacgca ccccagacaa aggccgatca gcaggaactg
     4921 ccacaactgt gtcgcgtaac tgaccagata gacgggcggc gcgcacgcca ccagcaaggc
     4981 ggtcatcatg ttgcgaccac caaaccggtc ggtccacgcc cccagcggca gccgcatgat
     5041 ggcgccggtg agcaccgggg tggcggtgag caggccgaat tccgtgctgt tcagccccag
     5101 ttcgtggcgg atggggatgc ccagcacccc gaacatcatc cagacgacaa agcagacgag
     5161 aaaagcgaac gtgctgacga acaacgtggt gtacgccctg gccggcgaca gcgcgggcgc
     5221 cgagggaggg gtttgcgaga gtgtggccat gtgcactcct ggaaacgtcg aggttcgtgg
     5281 tggggagagc gatcgccgca ggcgacacta caccgcctgc cgcaccaacc gcccaggtcg
     5341 actgcgatac aacaggaaaa tcacgatcgc ggcattgagc gcgttccacg cgatgccgtt
     5401 gaggaacgcg gcatgatacg aataggtcag atcgtagatg gcgccggaca tccagccgcc
     5461 cagcgccatg cccaccaacg tggccatcag cgccgtgccc acgcgcgcgc cagcgacttc
     5521 cggcgggaag tgttcgcgca cgatcaccgc gtaggacggc acgatgccac cctggaataa
     5581 tccgaacaag gcggacacca catacagcga caccaggcca tggttgaaca catacagcca
     5641 caatgccagc ccctgcaaca gtgagcccag caacagggtg cgcaacccgc cgatgcggtc
     5701 ggacagccag cccgacccca accggctgat gatgcccatg ctcagcatca gcgagagcat
     5761 gttggcgcca ttcgccgtgc catatcccag atcaccgcaa taagccacga tctgcacctg
     5821 cggcatggcc atggccacgc aaca
//
LOCUS       DASTNK010000133        16913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_104906, whole genome shotgun sequence.
ACCESSION   DASTNK010000133 DASTNK010000000
VERSION     DASTNK010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 16913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16913
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104906"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /gene="hisN"
                     /locus_tag="VFR20_09920"
     CDS             complement(<1..719)
                     /gene="hisN"
                     /locus_tag="VFR20_09920"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEU0230876.1"
                     /translation="MLGFAEDIATQVRAIALDWFRKPLTIDTKADESPVTIADRSVEQ
                     AIRQAITQRFPEHGILGEEYGLSHSEAEFVWSIDPIDGTRSFISGYPLWGSLLALLHR
                     GKPVLGVIDIPCTGERWTGSIHRGCLFSGRPCRTRTTRSLNEAILYTTTPDIFNGQEL
                     PAFERLSRAAHLRRFGGDCYNYGLLASGHIDLVLEAGLQPYDYLAMAPIIEQAGGIVT
                     DWSGNALTMHSSGQVLASANKA"
     gene            1026..2045
                     /locus_tag="VFR20_09925"
     CDS             1026..2045
                     /locus_tag="VFR20_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0230877.1"
                     /translation="MNISRKLVLASSLAMLGAVAARTAVAAGDVTIYTALEDDEVADY
                     MAVAKKDLPGINFHVLRLSTGDLGARLLAEARNPQADVVWGFAVTNMLDPRIQAMLTP
                     YEPAAAAKLPAMYKSSDKKWFAATGYMAALCVNTDRLKAKHLPMPTSWKDLTNPVYKG
                     ELVMPNPAASGTGYLQIAAMLQGLGDKAGWELIRDLNKNVAQYTSSGSKPCKMARTGE
                     YAVGASFAFPAMQSIESGYPIKMVIPEKYVGYELEASGLMKTAKDPKDAERFLAWTLS
                     PHAAELYKKYKEIITIPGVTPSEMSVKAGLPPDVSKVLYPMDFKASAKARDGIIKEWQ
                     KVTAK"
     gene            2131..3138
                     /locus_tag="VFR20_09930"
     CDS             2131..3138
                     /locus_tag="VFR20_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0230878.1"
                     /translation="MALTITNVSKRFDDFIALDNINLSVDEHEFVCLLGPSGCGKTTL
                     LRIIAGLLDMGDGQLMFGGRDLSAVPARERGFGIVFQSYSLFPNMTVAQNIGYGLRIR
                     GESKDTVRRRAGELLDLIKMPHLAERFPWQLSGGQQQRVALARALAVDPKLILLDEPL
                     SALDARVRNEMRMEIHDVQRRLRIPTIMVTHDQEEALMLADKIVCMNNGGIEQVGTPQ
                     DLYLRPSTRFVADFVGVSNLVGTAWVRRNAPMLLVDRPEGPDADYELCVRPENIGLTP
                     NPSGPARIDSVTFLGNLSRLRVDWDGQRFTAEGRMQATLIAGAPVSLSIDPADCCWVH
                     T"
     gene            3178..4875
                     /locus_tag="VFR20_09935"
     CDS             3178..4875
                     /locus_tag="VFR20_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0230879.1"
                     /translation="MNASMTLKTSPRGLGRLVPRSAEQWLVATCVFFPTLALVLFFVY
                     PLLTIVWESFTLKDGAIGLGNYEAISSTPGLLLAGWNSLVVSLSTTVVCVVVGFVLAF
                     ALDRTRMKGKQALRGTLLLPLLAPSLVQGLGLLFILGRNGLVHQWTGWNVNVYGYSGL
                     LLSDVFYALPQAVMIIQAALRNSDARYYDAASVMGATGWRQFFDITLPNCKFGLLSAG
                     FVVFTITITDFGNAAVIAGNYRVLATEIFNQVSGQQNFGMGSVVGIVLLLPSLVSVYI
                     ERVSSQKQFGSASESRIPVEPRPSAARDGSFAAISYFVMFCIIAVVAVVVFASFVKLW
                     PYNMSLTLANFNVNISSGYQPIWTTLYVSVAAAVIGVALLFCLAFGTKHVSGAPAKTI
                     YLLAVLPVGVPGLVMGLSYIFAFNNPSTPLYIFYGTAILIALCNFYHYHTQGFLTMMT
                     GMRAVPQALEEVISCLGGGVRRVLTDAVLPFIAPTVLSVFFFLFMRSMVTLSAVIFLV
                     TPELNVASVSVMHLNEAGFVSQAAAFSTIIILVVAVALIAMKLLVMRLTPRAARGAT"
     gene            4875..5642
                     /locus_tag="VFR20_09940"
     CDS             4875..5642
                     /locus_tag="VFR20_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEU0230880.1"
                     /translation="MWLEARHQRIQSLLDRLDRVTTDQVVSELGVSRETVRRDFLELE
                     AQGVLKRVHGGAVRIVDEPSIDQRTGIRVTHKQSVARALAQRLGPDLTVFLDAGSTTT
                     LLARELALLSHLTVITNSFDAAAALMANADSLRNNRVYFLGGDISQRLAATTGASTVA
                     EIGRFHVDMALLSPVAVDAVYGATSFDHNEALIARAMADNAQQVVILADYSKIGGRSR
                     ESYCVADHIDLLVTNANAVDRPGYDELAGMVSEILLA"
     gene            5866..6444
                     /gene="ribA"
                     /locus_tag="VFR20_09945"
     CDS             5866..6444
                     /gene="ribA"
                     /locus_tag="VFR20_09945"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEU0230881.1"
                     /translation="MNYITSCKLPTPWATFELHAFVEPETGKEHLAMTLGRVDDGEPV
                     LARVHSECMTGDVLFSQRCDCGAQLESALRHIAGGGRGVLLYLRQEGRGIGLLNKMRA
                     YRLQEQGVDTVEANEQLGFPADLRRYDLCVPMLAHLGIRQLRLMTNSPRKIALLRDAG
                     VDVVERIPLVVNRNPYNDAYLNTKAAKLGHWL"
     gene            complement(6536..7924)
                     /locus_tag="VFR20_09950"
     CDS             complement(6536..7924)
                     /locus_tag="VFR20_09950"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU0230882.1"
                     /translation="MNSLRARMALWVLLPLSIALSLSLWFSYQDSVNNARRQQDHTLW
                     ASAHIIASQIQWVNGQLSSTIPPVALEVFSSNSHDTVYLSVMTRDGRLLAGWPDLAAH
                     AGPPPEPPVSSYNNIEFHGMRLRAYTMSRDFFNSGKSMRVLITVAKTEREFLHNAHIL
                     WWPNLIRQSGVLVLVLVLMLWGLRHELKPLARLKDEVLSRERSDLRPIRLQALPLELR
                     PVVDTINQYGQRIQRHIDSRKRFIEDAAHQLRTPMTLLSTQLHYAASLATTPELKRIL
                     TALSDSRQQITLLVNQLLSLSQAENYRPESTPAQQLDVSDVARDVLVELAPLAASRDI
                     DLGIGTIFGPTPLRAPPALLRALVFNLVDNAIRYTPEGGHVTLTTSQPSAAPARLEVV
                     DTGPGIPPELRARVFERFNRGNATNQEGTGLGLAIVAEAAKACQASVTLGSGVNGKGL
                     RVVVEFRSPESE"
     gene            complement(7921..8595)
                     /locus_tag="VFR20_09955"
     CDS             complement(7921..8595)
                     /locus_tag="VFR20_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014895422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0230883.1"
                     /translation="MRLLLVEDNQELSRWLSELLRGEGFTVDCVHDAEAANVLLSGEH
                     YDAVLLDLKLPQASGQSVLRRLRRGGNDTPVLVLTASDSLDMKVDCLEMGADDYLVKP
                     FEMRELNARIRVLIRRRGGGGAPQLNCADLAYDTNTRQFSLNGAPLTLPLREHSLLEA
                     LLLNQGKTMSKTSLMQSIFGLDGDASEDAIEIYVHRLRRKLEASQASIVTLRGLGYLL
                     TPRHPS"
     gene            8796..9806
                     /locus_tag="VFR20_09960"
     CDS             8796..9806
                     /locus_tag="VFR20_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0230884.1"
                     /translation="MSSITRFLKYTAIASALATCAATSAHASEKITIMVGGINKIIYL
                     PAKLAENLGYFKDEGLDVVLLSEPAGVNAENEMLAGAVQGVVGFYDHSIDLQAKGKEV
                     ESVVQFSRVPGEVELVAASKAGEIKSPADFKGKTLGVTGLGSSTDFLTQYMAFKGGLK
                     HGDYTILPVGAGNTFIASIQQNRIDAGMTTDPTATQLITSGKAKVLVDMRTVSDTKKA
                     LGGLYPAASLYMPNAWVNSHKEEVQKLADAFVKTLKYIHTHSAAEIAAKMPKDYYAGN
                     EKLYVQALQASIPMFTADGKMPEGGPETVLKVLSTFKPAVKAAHIDLSKTYTTEFVSH
                     AK"
     gene            9875..10663
                     /locus_tag="VFR20_09965"
     CDS             9875..10663
                     /locus_tag="VFR20_09965"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0230885.1"
                     /translation="MQDQNTPAIDIGNVTCRFISADGKATIALQDFSMSVKRGEFCAI
                     VGPTGCGKSTTLSLITGLLRPTTGSVQVMGQPVNGIDPRIGFVFQNDAIFPWRNVIDN
                     VAAGPMFRGQGAKQAKALAYEWLRRVGLEKFPQHYPHQLSGGMRKRVALAQTFINNPE
                     ILLMDEPFSALDMQTRTVMQDELLQLWAASGGSVVFVTHDLEEAIALADKVIVLTSRP
                     ATVKHIYEIDLPRPRVMAEVRYDDAFIRYAKEIWDDLRAEVRIG"
     gene            10677..11558
                     /locus_tag="VFR20_09970"
     CDS             10677..11558
                     /locus_tag="VFR20_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0230886.1"
                     /translation="MSSQTESASLASQRANAVKDTLAQAEAEAQRLVKRRANLIIFWR
                     LAILFGVLGGWQLAASVGWIDPFFYSKPSLIALQIYDWIMHGTSQGPLWLQVAVTLEE
                     TLIGFFIGGAAGIIFGILLGRNQFLADIFSVYIKVANSIPRIVLGSIFVIALGLGMAS
                     KVALAVVMVFFVVFGNAFQGVREADRYMIANAQILGASPRQVTSQVVIPSAMSWILAS
                     LHVSFGFALVGAVVGEFLGARVGIGLLIANAQGAFNASGVFAAMIVLAIVGLLAEYLL
                     TYIEGRLLKWRPAQFSA"
     gene            complement(11718..12362)
                     /locus_tag="VFR20_09975"
     CDS             complement(11718..12362)
                     /locus_tag="VFR20_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU0230887.1"
                     /translation="MKLLHIDSSILGTNSASRELTANVVRQWVAQHPDTQVDYLDLAQ
                     DAPSHLNQESLGFRLSLDAAQLSDAQKHENAISEALVTQFLAADVVVVGTPLYNFSIP
                     SQLKAWIDRIAQAGRTFKYTESGPVGLAAGKTVIVVSSRGGVYSTSDAGQAMEHQESY
                     LKVVFGFFGITDVRIVRAEGLNMGEQTKAAAFSQAHDHIQAALVIEGETELAAA"
     gene            12552..13454
                     /locus_tag="VFR20_09980"
     CDS             12552..13454
                     /locus_tag="VFR20_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0230888.1"
                     /translation="MADLNDMRYFAEVVTRGGFAAAGRALEVPKSRLSRRIAQLEATL
                     GVRLLQRTTRKLSLTSVGEAYYRHCLAIVDEARAADEVVAQVSSEPRGLIRAVCPITL
                     AHSVARAILPDFMRRYPLVTVQMDVSNRVVDLVEEGVDVALRVRASLEESGSLIIKRL
                     GNSQTILVASPAQLRRQGAVSTLADLVRLDTVAMSVTDGRAAWSLYGPDGAEHVFTHR
                     PRYVADDLLTLKYAMMGGVGATMLPDYMCAEELARGEMVRLLPDLAPKPGVIHAVFVS
                     RRGMVPAVRAFLDFLGERLTSESL"
     gene            complement(13543..14505)
                     /locus_tag="VFR20_09985"
     CDS             complement(13543..14505)
                     /locus_tag="VFR20_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0230889.1"
                     /translation="MQREGSPWRGLAAVTLKELADHLSSARMRVLEWLVVLTAAAALY
                     GALQTIRQTTSSDPFVFLRLFTSTQAPLPSFASILGFLIPLVAIGLGFDAVNGEYSRR
                     TMSRILSQPIYRDALLLGKFIAGLATLAISLVCLWLLLIGSGLLLLGVPPSAEEVARS
                     LMFLVVALAYAGVWLAVSMLFSTIFKSAATSALVSLGLWLFLALLWPMLAPAVAHLIA
                     PPDLMSMLAGQPSLHTLHWQQALERISPTQLFGEAVVAILSPMTRSLGPVFLHQLEGL
                     VIGAPLPLAESLSIVWPQIIGLIAGCILLFAITYIVFQRQEVRA"
     gene            complement(14495..15451)
                     /locus_tag="VFR20_09990"
     CDS             complement(14495..15451)
                     /locus_tag="VFR20_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0230890.1"
                     /translation="MSAAVIDAANLTKRYGATTAVDGISFSVGAGEIFGLLGPNGAGK
                     TTTILMLLGLTEITDGSVRVLGLDPRREPLTIKQHLGYLPDSVGFYNQLSALDNLFYT
                     ARLLGIPAAQRLGRIHESLERVGLADAGHKRVSAFSHGMRQRLGLAEIIMKRARVAIL
                     DEPTSGLDPQATEDFLRLIRTLKADGVTVLLSSHLLDHMQRICDRVALFQQGRIALMG
                     TVPELARQVLGDGFEVEVEVSGERLADSLARVEGVSAVETIGADRYRLRANRDVRADA
                     ARAAVQAGGALRRLSIRDPSLDAIYNSYFQRPKVKEADSHAT"
     gene            complement(15448..16614)
                     /locus_tag="VFR20_09995"
     CDS             complement(15448..16614)
                     /locus_tag="VFR20_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HEU0230891.1"
                     /translation="MRKTLSLFLSLACLLFASAAMASDAPAQVRGIYLTTDYPSITVR
                     PGTTATVSLSLNNHHVAPTRMAVHVTNVPKGWKAQLLGGGQPVGAAMPGTGDSVDLEL
                     RLTVPSDAARNPKTLTVDADGGGLHLSLPIDVALANQLPAKLTLKSKLPELKGSTQSS
                     FSYDFTVKNESGKDMLVSLDAQVPQYFQTTFTEEYGTQELSSVPIKAGASKEITLKVQ
                     PPNTVEPGTYPIIMDVASGNAKASTTVNMQIVGQPRLALSGRNGIMSTHAEIDKATTM
                     PLILSNTGGADASGVRISGTAPSGWTIKFSPDKVEHLAPGKTKEIEATITPSSESLAG
                     DYMVDLQATSQGQSVDGNLRVSVVTSSMWGIIGAIIVAIAVLILVGAVARYGRR"
BASE COUNT         3068 a         5055 c         5423 g         3367 t
ORIGIN      
        1 gccttgttgg cggaggccag cacctgtccg ctggaatgca tggtcagggc gttccccgac
       61 cagtcggtga caatgccgcc cgcctgttcg atgatgggcg ccatggccag gtaatcgtag
      121 ggttgcaggc ccgcttcgag caccaggtcg atgtggcccg aggccagcaa cccatagttg
      181 tagcaatcgc cgccgaagcg gcgcaggtgc gcggcgcgac tgaggcgttc gaaggccggc
      241 agttcctggc cgttgaaaat gtccggtgtg gtggtgtaga ggatggcctc gttcagggat
      301 cgggtcgtgc gggttcgaca ggggcggccg gaaaacaggc agccccggtg aatcgaaccg
      361 gtccagcgct cgcccgtgca cgggatatcg atgacgccca atacgggttt gccacgatgg
      421 agcagcgcca gcagggatcc ccagagtgga tagcccgata tgaagctgcg cgtgccgtct
      481 atggggtcga tcgaccagac gaactccgcc tcggaatgac tcaggccgta ttcttcgccc
      541 aggatgccat gttccgggaa acgttgggtg atggcctggc ggatggcttg ttccaccgag
      601 cggtcggcga tcgtgacggg gctttcgtct gccttggtat ctatggtgag aggttttctg
      661 aaccagtcca gggcgatggc ccggacttgt gtggcgatgt cttcggcgaa accgagcatt
      721 tcatccagga tggcgtcgct tggcatggcg atatcgttgt cgggcttgat caccttgggt
      781 tccttgaaat catcatgaaa acaataagtc gcaaacgcat ttctatgttt ttgcctggga
      841 tgatccagat ttagtctaat cacacttgca aagatatgca aatttccaca aaataacaac
      901 atgcatcgag gggcgtcttg aaaaatattg cgtcaaccgt gcgtccgcca ccggccggag
      961 ggtctgggtg tgagctgtca caagagatga agtcaccgca tttcattcat aaatcaggag
     1021 acaacatgaa catttccaga aaactggtcc tggcttcctc gctggccatg ctgggggcgg
     1081 tggccgccag aacggcggtt gccgctggcg acgtcaccat ctataccgcg ctcgaggacg
     1141 acgaagtcgc cgattacatg gccgtggcca agaaagactt gccgggcatc aattttcacg
     1201 tgttgcgcct atccacgggt gatcttggcg cccgcctgct ggccgaagcc aggaatccgc
     1261 aggccgacgt ggtctggggg ttcgccgtca ccaacatgct cgacccccgt attcaggcga
     1321 tgttgacgcc ctacgagccg gccgctgcgg cgaaattgcc ggccatgtac aaaagcagtg
     1381 acaagaaatg gttcgccgcc accggctaca tggccgcgct gtgcgtcaac accgatcgct
     1441 tgaaggccaa gcatctgccc atgccaacat cgtggaagga tctgaccaat cccgtgtaca
     1501 agggcgagct ggtcatgccc aacccggcgg cctccggcac cggctatctg cagatcgcgg
     1561 ccatgctgca agggctcggc gacaaagcgg gctgggagct catcagggat ctgaacaaga
     1621 acgtcgcgca gtacaccagc tccgggtcga agccgtgcaa gatggcccgc acaggcgagt
     1681 acgcggtggg cgcctcgttc gcgttcccgg ccatgcaatc catcgagtcc gggtacccca
     1741 tcaagatggt gattcctgaa aagtatgtcg gctacgagct tgaggcctcg ggtttgatga
     1801 agaccgcgaa ggatccgaag gatgccgaac gctttctggc ctggaccctg tcgccgcacg
     1861 cggccgaact ctacaagaaa tacaaggaga tcatcaccat cccgggcgtg acaccgtcag
     1921 agatgtcggt caaggcggga ttgccgcccg atgtgtccaa ggtgctgtac cccatggact
     1981 tcaaggcctc ggccaaggcg cgcgacggca tcatcaagga atggcagaaa gtcaccgcca
     2041 aatgatgccc gtatcgtggc agtccggtgg ccgtccgcac ggccaccggg gccgaatcgc
     2101 aatcctgttc tctgatgggc gacagacttc atggccttga caatcaccaa cgtatcgaag
     2161 cggtttgatg atttcatcgc gctcgacaac atcaacctgt cggtcgacga gcacgagttc
     2221 gtctgcctgc tcggccccag tggctgcggc aagaccactt tgctgcgcat catcgcgggg
     2281 ctgctggaca tgggcgatgg tcagctcatg ttcggcggtc gtgacctcag cgccgtgccc
     2341 gcgcgtgagc gcggctttgg catcgtcttt cagtcgtact cgttgtttcc caacatgacg
     2401 gtggcgcaga acatcggcta tggattgcgc attcgcggcg aatcaaaaga cacggtgcgc
     2461 cggcgcgcag gcgaactgct ggatttgatc aagatgccgc atctggcgga acggtttcca
     2521 tggcaactgt cgggcgggca gcagcagcgc gtggcgctgg cgcgcgcgct ggcggtcgat
     2581 cccaagctca ttctgcttga cgaaccgctg tccgcgctgg atgcgcgtgt acgcaacgaa
     2641 atgcgcatgg agatccacga cgtgcagcga cgcctgcgca ttcccacgat catggtcacg
     2701 catgaccagg aagaggcttt gatgctggcc gataagatcg tctgcatgaa caacggcggc
     2761 atcgagcagg tgggcacccc gcaggatttg tacctccggc ccagcacccg ctttgtcgcg
     2821 gatttcgtgg gagtcagcaa tctggtggga accgcctggg tgaggcgcaa tgcgcccatg
     2881 ctgctggtgg atcgtccgga ggggccggat gccgattacg aattgtgcgt gcggcccgag
     2941 aacatcggcc tgacgccaaa cccttccggg ccggcccgca tcgattcagt gaccttcctg
     3001 ggcaatctca gtcggctgcg cgtcgactgg gacgggcaac gcttcaccgc ggagggtagg
     3061 atgcaggcca cgctcatcgc gggtgccccg gtcagcctga gcatcgatcc ggcggattgt
     3121 tgctgggtgc acacctgagc gcccatccat gaaccgactc tcccgcggaa gccattcatg
     3181 aacgcatcca tgaccctgaa aacaagcccg cgcgggctgg gccggctggt gccgcgcagc
     3241 gcggagcagt ggctggtcgc cacctgcgtg ttcttcccca cgctggcgct cgtgctgttt
     3301 ttcgtctatc cgttgctgac cattgtctgg gaaagcttca cattgaagga cggggcgatc
     3361 ggcctgggca actacgaggc catttcatcc acgcccggtt tgctcctcgc ggggtggaac
     3421 agcctggtcg tcagcctgag cacgaccgtg gtctgcgtgg tggtcgggtt tgtcctggcg
     3481 ttcgcgctgg accgcacccg catgaaaggc aagcaggcgt tgcgtggcac gctgctattg
     3541 ccattgctcg cgccctccct ggttcagggg ctgggcctgc tgttcattct cgggcgcaac
     3601 gggttggtgc atcaatggac gggttggaac gtcaatgttt atggttattc ggggttgttg
     3661 ctgtcggatg ttttttatgc gttgccgcag gcggtgatga tcatccaggc ggccttgcgc
     3721 aattccgatg cccgctatta cgatgcggcc tcggtcatgg gcgcgaccgg ctggcgccag
     3781 ttcttcgata tcaccttgcc gaactgcaag ttcggattgc tcagtgccgg cttcgtggtg
     3841 ttcaccatca ccatcaccga cttcggcaac gcggctgtca tcgccggcaa ctaccgcgtg
     3901 ctggcgaccg agatattcaa ccaggtgtcc ggccagcaga atttcggcat gggttccgtc
     3961 gtgggaatcg ttctgctgct gccatcactg gtgtcggtgt acatagaacg cgtgtcctcg
     4021 cagaagcagt tcggcagcgc gtcagagagc cgcattccgg tcgaaccacg gccgtcggcc
     4081 gcgcgcgatg gttcgttcgc cgccatctcg tatttcgtga tgttctgcat tatcgccgtg
     4141 gtggcggtgg tggtgttcgc cagttttgtc aagctctggc cctacaacat gagtctgacc
     4201 ttggcgaatt tcaacgtcaa catcagcagt ggctaccagc cgatctggac cacgctgtat
     4261 gtgtcggtgg cggcggctgt gatcggtgtg gcgttgttgt tttgcctggc tttcggcacc
     4321 aagcacgtgt cgggcgcgcc ggccaagacc atctacctgc tggcggtcct gccggtgggc
     4381 gtgccgggct tggtcatggg cctgtcgtac atcttcgcgt tcaacaatcc cagcacgccg
     4441 ttgtacattt tctacggcac tgccatactc atcgcccttt gcaatttcta tcactatcac
     4501 acccagggtt tcctgaccat gatgacaggt atgcgcgcgg tgccgcaggc gctggaagag
     4561 gtcatcagtt gcctgggcgg cggcgtgcgt cgggtgctga ccgatgcggt gctgcctttt
     4621 atagcgccca cggttctgtc ggtgtttttc ttcctgttca tgcgctctat ggtcacgttg
     4681 tcggcagtga tctttcttgt cacgccggaa ctcaatgtcg cctcggtgtc agtcatgcat
     4741 ctgaacgagg cgggtttcgt ctcgcaggcg gcggcgttct ccaccatcat cattcttgtc
     4801 gtggccgtcg cgctcatcgc catgaaactg ttggtcatgc ggttgacgcc gcgcgccgcg
     4861 aggggggcca cctgatgtgg cttgaggccc ggcatcaaag aatccagtcg ctgctcgacc
     4921 ggctcgatcg ggtgaccacc gaccaggtgg tcagcgaact gggtgtgtcg cgtgagacag
     4981 tgcgccgcga ttttctcgaa cttgaagcac agggcgtgct caagcgagtc cacggtggcg
     5041 ccgtccgcat cgtcgacgag ccctccatcg atcagcgcac cggcatccgg gtgacgcaca
     5101 agcaaagcgt cgcccgcgcg ctggcgcagc gtctggggcc cgatctgacg gtgtttctgg
     5161 acgcgggcag cacgaccacg ttgctggcca gggaactggc tttgttgtcg cacctgacgg
     5221 tgatcacgaa ttcgttcgac gccgccgcgg cgctcatggc caacgccgat tcgctgcgca
     5281 ataatcgtgt ctatttcctg ggcggggata tcagccagcg tctggcggcc accaccggcg
     5341 cgtccaccgt cgccgagatc ggccgtttcc atgtggacat ggccttgttg tcacccgtgg
     5401 cggtcgatgc ggtgtatggc gcgaccagct tcgaccacaa cgaagccctg atcgcgcggg
     5461 cgatggccga caatgcacaa caggtggtga tcctggccga ctacagcaaa atcggtggcc
     5521 gcagccgtga gagctattgc gtcgcggatc atatcgattt gctcgtgacc aacgcgaatg
     5581 cggtcgatcg gccgggttat gatgagttgg ccggcatggt gtccgaaatt ctgctggcct
     5641 gatttctccc cgttcggcga atacggcagg aattctggcg ctcctggccc ggccggccgg
     5701 tattcaattg tctcgcccgt ggttttcacc gtcccgatcg tgcggcgggt gccggcggcc
     5761 aaacctgcta ttcttgtcac ctcgcgcctg cctgttgtcg cggttgaccg tggccgggct
     5821 gtcgggcgcg tggttcgttc tgtcgtccag tccaagagaa accccttgaa ttacatcacc
     5881 tcgtgcaaac tgcccacgcc ctgggccacg tttgaactac atgcctttgt cgagcctgaa
     5941 accggcaagg aacatctggc catgacgctg ggccgggtcg atgacggcga gcccgtgctg
     6001 gcgcgcgtgc attccgaatg tatgaccggt gacgttctgt tcagccaacg ctgtgattgt
     6061 ggcgcgcaac tggagtccgc gctgcggcat atcgccggtg gcgggcgcgg ggtgttgctg
     6121 tatctgcgcc aggaggggcg gggcataggc ctgctcaaca agatgcgggc gtaccggctt
     6181 caggaacaag gtgtggacac ggtcgaggcc aatgagcaac ttgggtttcc ggccgatctg
     6241 cgccgctacg atttgtgtgt gcccatgctg gcccatctgg gcatccggca attgcggctc
     6301 atgaccaaca gcccgcgcaa gatcgcattg ctgcgtgatg ccggcgtcga cgtggtggaa
     6361 cgcattccgt tggtggtcaa tcgcaacccc tacaacgacg cctacctcaa caccaaggcc
     6421 gcgaagctgg gccactggct atagcaacgg gccttcatcg cgagctggcc gcgtgccgtc
     6481 gtgccgcggc gggtcggcct tgtcgcgggc aaggacttgg gcgggatcga agctgtcact
     6541 ccgattcggg cgagcggaac tcgacgacga cacgcagccc ctttccgttc acacccgagc
     6601 ccagcgtcac cgaggcctga caggctttgg cggcttcggc gacgatcgcc agccccagac
     6661 ccgtaccttc ctgattggtg gcgttgccgc gattgaatcg ttcgaacacg cgcgcgcgca
     6721 attccggcgg gattcccggc ccggtgtcga caacctcgag ccgggccggg gcggcggacg
     6781 gctggctggt ggtcagtgtc acgtgtccac cttccggggt atagcggatg gcattgtcga
     6841 ccaggttgaa gaccaaggct cgcaggaggg cgggcggggc gcgcagcggt gtcggcccga
     6901 atatcgtgcc aattcccagg tcgatatccc ggctggccgc aagaggggcc aattcgacca
     6961 gcacgtcccg cgccacgtcg ctgacatcca gttgttgcgc cggtgtggat tccggacggt
     7021 aattttcggc ctgcgacagg ctgagcaact gattgacgag cagcgtgatc tgctggcggc
     7081 tatcgctcag cgcggtgaga atccgcttga gttcgggggt cgtcgccaag ctggcggcgt
     7141 agtgtagttg tgtgctgagc agcgtcatgg gagtgcgcag ttgatgcgcc gcgtcctcga
     7201 tgaaccgctt gcgggaatcg atgtgccgct gaatgcgctg gccatactga ttgatcgtgt
     7261 cgaccaccgg tctgagttcc aggggcaggg cctgcaggcg gatggggcgc aggtcggagc
     7321 gttcgcggct cagcacctcg tctttgagtc tggccagtgg cttgagttca tggcgcaggc
     7381 cccacaacat gagcaccagc accaggacca ggacgccgct ttggcggatc aggttgggcc
     7441 accacagaat gtgggcgttg tgcaggaact ctctttccgt cttcgccacc gtgatgagta
     7501 cccgcatcga tttcccagag ttgaagaaat cgcggctcat ggtgtaggcg cgcaaccgca
     7561 tgccatggaa ttcgatgttg ttgtaggacg aaaccggggg ttccgggggc ggcccggcgt
     7621 gtgccgccaa atccggccag cccgccagca agcgcccgtc gcgtgtcatc acggagaggt
     7681 acacggtgtc gtggctgttg gaggaaaaca cttccagcgc cacgggcgga atcgttgagg
     7741 aaagttggcc attcacccat tggatctggc tggcgatgat gtgcgccgac gcccacaaag
     7801 tgtgatcctg ctggcgccgc gcgttgttca cgctgtcctg gtacgagaac cagaggctca
     7861 gtgacagcgc gatcgagagg ggcaaaagca cccacagcgc catgcgggcc cgcaagctgt
     7921 tcatgacggg tgtctgggcg tcagaagata acccaggccg cgcagtgtga cgatggaggc
     7981 ttgggaagct tcgagtttgc gccgtaatcg gtgcacgtag atttcgatgg catcctcgct
     8041 ggcgtcgccg tcgaggccaa agatgctttg catcaggctg gtcttgctca tggtcttgcc
     8101 ctgattcagc agcagagcct cgagcaggct gtgttcgcgc aacggcagcg tgaggggcgc
     8161 gccgttcagg ctgaactgcc gtgtgttggt gtcgtaggcc aggtcggcgc aattgagttg
     8221 cggtgcgcca ccgccaccac ggcggcggat gagcacgcgg atgcgggcgt tgagctcgcg
     8281 catttcaaag ggtttgacca ggtagtcgtc agcccccatc tccaggcagt ccaccttcat
     8341 gtcgagtgag tcgctggccg tcagcaccag cacgggcgtg tcgttgccgc ctcggcgcag
     8401 gcggcgcaac acactctggc cgctcgcctg ggggagtttc aggtcgagca gcacggcgtc
     8461 gtaatgctcg cccgagagca gcacattggc ggcttccgca tcgtgcacac aatcgacggt
     8521 gaatccttcc ccgcgtagca gctcggacag ccagcgcgac agttcctggt tgtcttcgac
     8581 gagcagcagc ctcatgggaa cgacctttgg tgtgcggcca ccgcgaagca cgggtttccc
     8641 ctagtgtttt caggctggcg gcgaaagctg gatgaaagtt ggccaagtct agcattgatg
     8701 gtactgcgag cctcaagtgc gccgtcccgc aaatccttgt ggaacagcct ccaggccgca
     8761 tgcaggataa atcgtccatc aatcaggaga cttacatgtc cagtatcacg cgttttctga
     8821 aatataccgc catcgcctcc gcgttggcga cttgtgccgc gacgagtgcg cacgccagtg
     8881 aaaaaatcac catcatggtc ggcggtatca acaagatcat ctacctgccc gccaagctgg
     8941 ccgagaacct ggggtatttc aaggatgagg gcctggatgt ggtgctcttg tccgagcccg
     9001 ccggggtcaa cgccgaaaac gaaatgctgg ccggcgcggt acagggtgtg gtgggcttct
     9061 atgaccattc catcgatttg caggccaagg gcaaggaagt cgagtccgtg gtccagtttt
     9121 cgcgcgtgcc gggcgaggtc gaactcgtcg ccgccagcaa ggcgggcgaa atcaaaagcc
     9181 cggctgattt caagggcaag acgctcggtg tcaccggcct gggttcgtcc accgacttcc
     9241 tgacgcaata catggcgttc aagggtggcc tcaagcatgg cgactacacc attctgccag
     9301 tgggcgcggg caacacgttc atcgcgtcca ttcagcagaa ccgcatcgat gccggcatga
     9361 ccaccgaccc gaccgccacg caactgatca ccagcggcaa ggccaaggtt ctggtcgaca
     9421 tgcgcaccgt gtcggacacc aagaaggcgc tcggcggctt gtatccggcg gccagcctgt
     9481 acatgcccaa cgcctgggtg aacagccaca aggaagaggt ccagaaactg gccgacgcct
     9541 tcgtcaagac gctgaagtac atccacacgc attcggccgc tgagatcgcc gccaagatgc
     9601 ccaaggatta ctacgccggc aacgagaagc tctacgtcca ggcgctgcag gcgtccattc
     9661 cgatgttcac cgccgacgga aaaatgcccg agggcgggcc tgagaccgtg ctgaaggtgc
     9721 tgtccacgtt caagccggcc gtaaaggcgg cgcatatcga tctctcgaag acctacacca
     9781 cggaattcgt gagccacgcc aagtaatcgc cagacacgag aaatggggca tcgccggaac
     9841 gcctgcctcg tttctcccaa tccggacaaa cgcaatgcaa gatcaaaaca ctcccgccat
     9901 cgacatcggt aacgtcacat gtcgcttcat ctcggccgat ggcaaagcca ccatcgcgct
     9961 gcaggatttc agcatgagcg tcaagcgtgg cgagttctgc gccatcgtcg gccccacggg
    10021 ttgcgggaaa tccaccacgc tgagcctgat caccggtctt ctgcgaccca ccactgggtc
    10081 tgtccaggtg atgggccaac ccgtgaacgg tatcgacccg cgtatcggct tcgtgttcca
    10141 gaatgacgcc attttccctt ggcgcaatgt catcgacaac gtcgccgccg gcccgatgtt
    10201 ccgggggcag ggtgcaaagc aggcgaaggc cctggcctac gagtggctgc ggcgggtcgg
    10261 cctggaaaaa ttcccgcaac attatccgca tcagctttcc ggcggcatgc gcaagcgcgt
    10321 ggcgctcgcc cagacgttca tcaacaaccc ggaaatcctg ctgatggatg aacctttcag
    10381 cgccctggac atgcagaccc gcaccgtcat gcaggacgaa ctactgcagt tgtgggcggc
    10441 cagtggcgga tcggtggtgt tcgtcacgca cgacctggag gaagccattg cgttggcgga
    10501 caaagtcatc gtgctcacgt cccgccccgc aaccgtgaag catatttatg aaattgatct
    10561 gccccgtccg cgcgtgatgg cggaagtgcg ctatgacgac gcgttcatcc gttacgcgaa
    10621 ggaaatctgg gacgatctgc gagccgaggt ccgaattggt tgagggaact attgccatgt
    10681 ctagtcaaac tgaatccgcg agcctggcca gccagcgcgc caatgccgtg aaagacacgc
    10741 tcgcccaggc ggaagccgag gcacagcgcc tggtcaagcg ccgcgccaat ctcattattt
    10801 tctggcgcct cgccatcctg ttcggtgttc tgggcggctg gcaactggcc gcctcggtcg
    10861 gctggataga tccattcttc tattccaagc cgtcgctcat cgccctgcag atctacgatt
    10921 ggatcatgca tggcacatcg caggggccgt tgtggctgca ggtggcggtc acgctggaag
    10981 aaaccctgat cggtttcttc atcggtggcg cagccggcat cattttcggc attctgctgg
    11041 ggcgcaacca gtttctggcc gacattttca gcgtctacat caaggtcgcc aactccattc
    11101 cccgtatcgt gctgggctcc attttcgtca ttgccctggg cctgggcatg gcgtccaagg
    11161 tggcgctggc ggtcgtcatg gtgtttttcg tggtgttcgg caacgctttc caaggagtgc
    11221 gcgaggcgga ccgctacatg atcgccaacg cccagatact gggcgcctcg ccgcgacagg
    11281 tgacgagcca agtggtgatt ccctcggcga tgagctggat tctggccagt ctgcacgtca
    11341 gcttcgggtt cgccttggtg ggcgcggtgg tgggtgagtt cctgggagct cgggttggta
    11401 tcggcctgtt gatcgccaac gcccagggag cgttcaatgc cagcggtgtg ttcgccgcca
    11461 tgatcgtgct ggccattgtt ggcttgctgg cggaatacct cctcacttat atcgaggggc
    11521 ggctgttgaa atggcgcccg gcgcagttct cggcctgatc gccacgcccc gccacatcag
    11581 acatctgtcc gatcttcaac accaacgaga cacggcgttg tgtatgagaa gccggctcag
    11641 gacactggcg catagcatga gtcacctgag ccgggatggg gcgcgtgatc cgccgcgccc
    11701 tttggcgttg gaagctctca ggcagccgcc aattccgttt cgccctcgat gacaagcgcg
    11761 gcttggatgt gatcgtgcgc ctgcgagaag gcggccgcct tggtttgttc ccccatgttc
    11821 agcccttcgg cgcggacgat gcgcacatcg gtgatgccga agaacccgaa caccactttc
    11881 aggtagcttt cctggtgttc catggcctgg cccgcatccg atgtggaata aacgccaccg
    11941 cggctcgaaa cgacgatgac ggttttgccg gcggccagac ccaccggacc tgattccgtg
    12001 tacttgaacg tgcggcccgc ctgggcgata cgatcgatcc aggccttgag ctggctgggt
    12061 atcgagaagt tgtagagcgg ggtgcccacc accacgacgt ctgccgccag gaattgtgtc
    12121 accagcgctt cggagatggc gttctcgtgt ttctgggcat cactcagttg cgccgcgtcc
    12181 agactcagac ggaagcccag tgattcctgg ttcaggtgcg agggcgcgtc ctgggctagg
    12241 tccaggtaat ccacttgcgt gtcgggatgt tgcgccaccc actggcgcac gacgttggcg
    12301 gtgagttcgc gggaggcgga gttcgttccc aggatactgg agtcgatatg cagcagtttc
    12361 atgcttcttc ctttctgatg gtgtcggagg gtcattggcg atgtcggcct gagtgcggcc
    12421 gcttgccgat gaaaaattct tcagaagaag tatcactcac ctcatcggta ttgataagat
    12481 tgcctaattg caataggttg tcctgaattt gagtctaata tgccgggcat atcaggaagg
    12541 agaagggaat catggcggac ctcaatgaca tgcgatattt cgctgaagtg gtcacgcggg
    12601 gcggttttgc cgctgcgggg cgggcgctgg aggtgccgaa atcgcgtctg tcccggcgta
    12661 tcgcgcagct cgaggcaaca ctgggcgtgc ggctcttgca acgcaccacg cgcaagctgt
    12721 ccctgacaag cgtgggcgag gcctattacc ggcactgcct ggccatcgtg gacgaggcca
    12781 gggcggccga cgaggtggtg gcacaagtga gcagcgagcc gcgcgggctg atccgtgccg
    12841 tctgcccgat cacgctggca cacagtgtcg cccgcgccat cctgcctgac ttcatgcggc
    12901 gttatccact ggtgacggta cagatggacg tcagcaaccg ggtggtcgat ctggtggagg
    12961 aaggcgtgga tgtggcgttg cgggtgcgcg cgtcactcga ggaaagcggc agcctgatca
    13021 tcaagcggct ggggaattcg cagacaatac tggtggccag ccccgcccag ctacggcgcc
    13081 agggcgcggt gtccaccctg gccgatctgg tccggctcga taccgtcgcc atgtcggtca
    13141 ccgatggtcg cgctgcctgg agtctgtacg gtccggacgg cgccgagcat gtgttcacgc
    13201 acaggccgcg ctatgtggcc gacgacttgc tcacgctcaa gtacgccatg atgggagggg
    13261 ttggggcgac catgctgcct gattacatgt gcgcggaaga actggcgcgg ggggagatgg
    13321 ttcggctgct gcccgatctg gcccccaagc cgggcgtgat ccatgccgtg tttgtgtcgc
    13381 gtcgcggcat ggtgccggcg gtgcgcgcat tcctggactt cctgggagaa cgtttgacgt
    13441 ctgaatcatt gtgagtgata ccgaggcgcg cacccgacga atggcgcgcg cactcgactc
    13501 atcactcgct ccttgggggg gagtctcgtt ctcgatcgcc gctcaggcgc gaacctcctg
    13561 gcgctggaag acaatatacg tgatggcgaa cagcagaatg cagccagcaa tcagtccgat
    13621 gatctggggc catacgatgg acaggctttc ggccaacggc agcggcgcac cgatcaccag
    13681 accctccaac tgatgcagaa acaccgggcc cagcgaacgc gtcatggggc tgaggatggc
    13741 caccacggct tcaccgaaca actgcgtggg tgaaatccgc tccagcgcct gctgccagtg
    13801 gagcgtgtgc aggctgggct gtccggccag catcgacatg agatcggggg gcgcgatcag
    13861 atgtgccaca gccggcgcca gcatgggcca cagcaacgcc aggaacaacc acaggcccag
    13921 cgataccaat gccgaggtcg ccgccgattt gaagatcgtg gaaaacagca tggataccgc
    13981 cagccagacg ccggcgtagg caagcgccac gaccaggaac atgagcgaac gagccacctc
    14041 ttcggcgctg ggtggcacgc ccaggagcag caggcccgag ccaatcagca gcagccagag
    14101 acacaccagg ctgatcgcca gggtcgccag tccggcgatg aacttgccca gcagcagcgc
    14161 gtcccggtaa atgggctgcg acaggatgcg gctcatggtg cgccggctgt attccccatt
    14221 gacggcgtcg aaccccagcc cgatcgccac cagcgggatc aggaaaccca gaatggaggc
    14281 gaacgagggc aggggcgcct gcgtgctggt gaacaggcgc aggaacacga atgggtcgga
    14341 ggatgttgtc tggcgtatgg tctgcagcgc gccatacagc gccgccgccg cggtcagcac
    14401 caccagccat tccagcacac gcatgcgcgc gctggataga tgatcggcca gttctttcag
    14461 cgtgaccgcc gccagccctc tccagggtga accttcacgt tgcatggctg tctgcctctt
    14521 tgacttttgg gcgttggaaa tagctgttgt agatggcgtc caggctggga tcgcggatcg
    14581 acaggcgccg caacgcgcca cccgcttgca ccgcggctcg cgcggcgtcc gcgcgcacgt
    14641 cccggttggc gcgcaaccgg taccggtccg ctccaatcgt ctccacggcg ctgacgcctt
    14701 cgacgcgcgc caggctatcg gccaagcgct cacctgaaac ttccacttcg acctcgaagc
    14761 cgtcgcccag cacttggcgc gcaagctccg gcaccgtgcc catgagcgcg atcctgcctt
    14821 gctggaacag cgccacccgg tcgcagatcc gttgcatgtg atccagcaag tgcgacgaca
    14881 gcagcacggt gacgccgtcg gctttgagcg tgcggatcag tcgcaggaaa tcctcggtcg
    14941 cttgcgggtc cagtcccgat gtgggttcgt cgagtatggc gacgcgtgcg cgcttcatga
    15001 tgatctcggc aagccccagg cgctgacgca tgccgtgcga gaacgccgac acgcgcttat
    15061 ggcccgcgtc ggccagtccg acgcgctcca gggattcgtg gatacgcccg agccgttgtg
    15121 ccgccgggat tcccagcagg cgcgcggtgt agaacaagtt gtcgagcgcg gacaactggt
    15181 tgtagaaccc gacggaatcc ggcaggtatc cgaggtgctg cttgatggtg agcggttccc
    15241 gcctggggtc gaggcccagc actcgcacgc tgccgtcggt gatctcggtc agaccgagca
    15301 gcatcaggat ggtcgtggtc ttgcccgcgc cgttggggcc cagcaatccg aagatttcgc
    15361 ccgcgcccac gctgaacgag atgccgtcca ccgcggtcgt ggcgccatac cgtttggtca
    15421 ggttggcggc gtcgatgacg gcggcgctca tcgacgtcca taccttgcca cggcgccgac
    15481 caggatcagc acggcgatgg cgacgatgat cgcgccgatg atgccccaca tgctcgacgt
    15541 gaccaccgac acacgcaggt tgccatccac cgattggccc tgagacgtgg cctgcagatc
    15601 gaccatgtag tcgccagcca gcgattcgga cgacggcgtg atggtggctt cgatttcctt
    15661 ggtcttgccg ggagccagat gctcgacctt atcgggcgag aacttgatgg tccagcctga
    15721 tggcgcggtg ccggaaatcc tcaccccgct ggcgtcggcg ccaccggtgt tgctgaggat
    15781 cagtggcatc gtggtggcct tgtcgatctc ggcatgggtg ctcatgatgc cgttgcggcc
    15841 ggacagcgcc aaccgcggct ggcccacgat ctgcatattc acggtggtgc tggctttggc
    15901 gttgccggag gcgacgtcca tgatgatcgg gtacgtgccc ggctcgaccg tgttgggggg
    15961 ttggactttg agcgtgatct ccttggacgc gcccgccttg ataggcacgg aactgagctc
    16021 ctgcgtgcca tattcttcgg tgaatgtggt ctggaaatat tgaggcactt gcgcgtcaag
    16081 gctcacaagc atgtccttgc cgctctcgtt cttgacggtg aagtcatagc tgaacgacga
    16141 ctgcgtgctg cccttcagtt ccggcagctt ggatttcagc gtgagcttgg ccggcaactg
    16201 gttggcgagc gccacgtcta tcggcaggct cagatgcagt ccgccgccat cggcatccac
    16261 cgtcagtgtc ttggggttcc gggcggcatc ggaagggacg gtcaaccgca gttccagatc
    16321 cacgctgtcg ccggtgccag gcatggcggc acccacgggt tggccgccgc cgagcaattg
    16381 tgctttccag cccttcggca cattggtgac gtgaaccgcc atccgagtcg gggcgacgtg
    16441 atggttgttc aagctcaagg acacggttgc ggtggtgccg gggcgcaccg tgatcgacgg
    16501 gtagtccgtg gtcaaataaa tgccccgcac ttgcgccggg gcatcgctgg ccatggcagc
    16561 cgaggcaaac agcaggcagg ccagggaaag gaaaagggaa agcgttttgc gcatggcgac
    16621 gtccaactcc ggttgttggc atggcttcgc ctgatcgccc gtgcgcgacc atgccgttgt
    16681 tgtcaaaagt caggtcggga cgttgcggcc cgttgaggtg ggtgcgttgc gcgaagacgc
    16741 ggtcgctgcc gtgtcagggc gggacggctc gacgctccgg cctggaaggc acggactcct
    16801 tatatcgagc gtgtcctgga acgcacgcgg ggtcgcaagc gtcattgaca acgtaaagac
    16861 ggatcggacg gttgtttagt tccgcttata ttttttcaaa atgtttcatg aag
//
LOCUS       DASTNK010000134        15984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_105023, whole genome shotgun sequence.
ACCESSION   DASTNK010000134 DASTNK010000000
VERSION     DASTNK010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15984
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105023"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..1727)
                     /locus_tag="VFR20_10000"
     CDS             complement(210..1727)
                     /locus_tag="VFR20_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEU0230892.1"
                     /translation="MITLSIMLATIMQTLDSTIANVALPHMAGSLSASQDQIMWVLTS
                     YIVSAAIATPVTGWLSGRYGRKAIFLYSIAGFTASSLLCGMSMSLLEIVCARLLQGMF
                     GAALVPLSQSVMLDINPPERHARAMAVWGLGVMLGPVLGPTLGGWLTDNFSWRWVFLI
                     NLPVGILSFLGVLYFIHERGNKQSARFDWFGFSLLAIAIAMLQLILDRGDQKDWFGSI
                     EICTEAVAGVIALTFFVLHTITDTGHSFFNVKLLRDRNFVTGLAFYFLLGLLLYATRA
                     LLPPLLQNLMGYPVMATGLVTAPSGLGTMLTMMLAGRLVGRMDSRLILAMGFGLTAAS
                     LWQMSNYTLQINDWDIVWPGFIQGMGIGFTSVPLTTMTFSTLDRALRPEGTAIYSLSR
                     NIGSSIGISAMQTLLVRNTARMHSTLAAYVTAGAVMLDQSGLTRLFDPGTAAGLAGLN
                     QVVTRQAAFIGYIDDFRLMFWMTLLSMPCLLLMRETKRATDKAPDPAEAIAAAAE"
     gene            complement(1822..2283)
                     /locus_tag="VFR20_10005"
     CDS             complement(1822..2283)
                     /locus_tag="VFR20_10005"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0230893.1"
                     /translation="MDDLSRRLSSRRALRESVVARLLQHVATKLGDYLDASLRDDGLN
                     TTLWMSLLLIYASPDHRLKPSELSVFMNSSRTNSTRVSRELQRHGLVDSIPDEHDKRQ
                     QFLELTLKGSTFVEHALPARRQHVQHALGGLTPAEIAELERLLHKLLHRVD"
     gene            complement(2474..3424)
                     /locus_tag="VFR20_10010"
     CDS             complement(2474..3424)
                     /locus_tag="VFR20_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU0230894.1"
                     /translation="MALHIETPYIESRPLSLGTGKSVWLKLEALQPTGSFKARGMGHA
                     CLIHKQHGAQRFISSSGGNAGLAVAYAGRQLKVPVTVVVPESTSERAKALIAMEKGVV
                     VVHGKNWGEAHQAAKAMVDAHSVLLHPFDDPLLWSGHSTMIDEMVRQGPRPDAIILSV
                     GGGGLLCGVIEGLQKSSWGGVPVVAVETVGADCLAQSLQAGEPVTLDDITTVATSLGA
                     RRACDRALDLSRSHSVESVVVQDRQAVEACVRFMDDHRLIVEPACGASLAALFSNADS
                     LRRATTIAVVVCGGVGSTAANLAALHERLAESADTTATPS"
     gene            3620..4270
                     /locus_tag="VFR20_10015"
     CDS             3620..4270
                     /locus_tag="VFR20_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEU0230895.1"
                     /translation="MTRQLFYYPGNASMAPHIILEELGVPHELSLVDRAGGGHKSPEY
                     LKLNPNGLIPVLVDGDMVLYESAAICLYLCDAHPQSGLAPALGSTERAHFYKWLIWMT
                     NTLQASLLGYFYPERWVDDGNATGAAQVRAHAETRIGGLLDQMEAQLENSGGPWFLGQ
                     RFTAVDAYAVMLCRWTRGFERPARALPLLGAYLSRVLERPAVRRTLTSEGLTAPWV"
     gene            4365..5405
                     /locus_tag="VFR20_10020"
     CDS             4365..5405
                     /locus_tag="VFR20_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEU0230896.1"
                     /translation="MSARQRKKNREGQAIILIIQTLVAALVALWGGLALWVQQPGGRL
                     CAKGLIVLWVLAALAFMAVPWLASPTSVKLAGRLIFPALVALVLVWWAGIRPSNDKNW
                     IPEVSRPLQGTVDGDVVILENVRNFLWRSADDCTPRWETRHYDLNTLRSVDLSLSYWA
                     GPAVAHVMVSFGFGDGGYVAFSVEIRRKVGDKFSEIGGFFRQYELSIVAADERDILRV
                     RTNVRGEDGYMYRVHMPLAAARSLFLAYVDRANRLVARPRFYNTVTANCTTLVYQMMD
                     RIVPGLPMSYQLLASGYLPEYLYRLDALAGASDLAEYRRRGRYTDRALANTDLDRFSH
                     DIRRGVPGIDAA"
     gene            complement(5420..6367)
                     /locus_tag="VFR20_10025"
     CDS             complement(5420..6367)
                     /locus_tag="VFR20_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEU0230897.1"
                     /translation="MPAFLSRLRTLLLICSLTTLCLPWPGARGTVRAAETLDNTPRIA
                     VISAFQPELSLLLKHVQAPHRFSANGVEFTTGSLQGKPVVLFLSGISMTNAAMTTQLA
                     LDRFHITHIVFSGIAGGVNPALHIGDVTVASRWGQYLELLMARETAPGVFTPPPGKDT
                     LHLPHYGMMFVRPVRVRSAAHPQLESKFWFDADPHMLAIAKGLHDVTLGACDKRGHCL
                     EKSPRLVVGGNGVSGSAFVDNAAFRRYVFDTFHANVLDMESAACAAVAYSNGVPFLAF
                     RSLSDLAGGGPGANEMHTFLDIAADNSAKVLMAFLAAWH"
     gene            6681..8081
                     /locus_tag="VFR20_10030"
     CDS             6681..8081
                     /locus_tag="VFR20_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEU0230898.1"
                     /translation="MARLMFLLLGVGYLRRRWRVLLVLGVIWLLAGGLIVGDAMDGVL
                     YFPLKFFAYLCLLEGLSTLAIATTGVGGQRILRYVKGVAAVVAAILVLVDSRHGHFLL
                     AMIFGTLFLIDGVMQCVAAYVVRFSRWRQTLGIGIFEIVLAILFYQPYPTHYVGTLPI
                     ALGFFLIMAGIKLVNLAVRVRRIKTNPGLGESPTDGLLPENLWGAPNQKPVSVWDGPP
                     ADDESALTVHVWTPTGSSKAQAVRYPIVDRYIAAVDVNGVISTGHAALESPEGIYISL
                     YPAEEIDRSPDQFGRLLRATSDNDVSGRFLPNYGVESREWCPSTVRVRIRNYDPAKLA
                     AFWGQYRQNATYNLTHRNCSSTVANALDAALDGCVGRLHRHGHGWGGFLRLLVTPELW
                     VAAQVRKRAHTMAWTPGLTLDYARAMSMLADPRPFGWWKMFQLAARRIMRLRRQWRAA
                     DRQVGQAPSAVAGPEA"
     gene            complement(8177..8818)
                     /locus_tag="VFR20_10035"
     CDS             complement(8177..8818)
                     /locus_tag="VFR20_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEU0230899.1"
                     /translation="MYVPTHFDESRPEVLHEFITDHPLGVLITHGDGGLDANHIPFEL
                     DAKTGSHGVLHGHVARANPLWTSVHDGDEVLVVFRGENGYISPSWYPSKHENHKSVPT
                     WNYRVAHAHGRITVRDDERYVRGVVARLTRTHEAREAKPWKMTDSPPEYIDTMLKAIV
                     GIEIHITCLEGKFKLSQNREARDLRGAADALLERGQADLGRTMLDQASQTKTA"
     gene            complement(8893..9645)
                     /locus_tag="VFR20_10040"
     CDS             complement(8893..9645)
                     /locus_tag="VFR20_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU0230900.1"
                     /translation="MNHRAPSSDDRAMLDGMVAHFALLEPVFDAMPDVVFFVKDTEAR
                     YVLANRTLASRCGFRDKRDLLGKTAEQVFPARFGRIYTAQDKAVVAHGGKLIDQLELH
                     LYPGREPGWCLTCKLPLRDAQGRILGVAGISRDLQAAEGRHPAYQRLATVARHIQDNY
                     SQPLELARLAGLANMSIAQIERYFRKIFHLTPRQLLLKTRLDAASTLLAGDASITDVA
                     TRCGYNDHSAFTRQFKATVGLTPSQYRALISG"
     gene            complement(9642..10955)
                     /locus_tag="VFR20_10045"
     CDS             complement(9642..10955)
                     /locus_tag="VFR20_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0230901.1"
                     /translation="MTEPTFHFDILVAGAGPAGLSAARIAADSGARVGIIDDNPRPGG
                     QIWRQGPDHPPVGQARTLLRALADRPNVSLHSGTRIVQALPGPRLMVENARQSLTLTG
                     DRVIIATGARERFLPFPGWTLPGVTGAGGLQALIKGGTPVEGQRIIVAGSGPLLWAAA
                     ATAQACGARIVAIVEQAPARAVARFAGGLLLTPAKLVQAMRLRLGLRRTPYWRDAWIT
                     AAHGDAALDRVTIRQRGAAFDVPCDRLACGFGLLPNTRLAAAMGCRLTPHAGDPAVAV
                     DALQATSVDRVFAAGECTGVGGMELSATEGRIAAHAALGRPEDARKLFPLRERYRRFA
                     ARMHEAFALNPALRTLAAPDTIFCRCEDVAHHDVAAQTSWRAAKLQTRCGMGPCQGRI
                     CGAAAAFCFGWGPEGENVGTPPRAPLSPARVATLMRPLDEPESPT"
     gene            complement(10952..11218)
                     /locus_tag="VFR20_10050"
     CDS             complement(10952..11218)
                     /locus_tag="VFR20_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU0230902.1"
                     /translation="MPETIVLFIDGHPVTVTAGSTVAAAIALATGGASATTRRSVGGA
                     LRGPLCGMGVCQECRVEIDGHAHRLACQTLCAADMQVITGAVSA"
     gene            complement(11211..12383)
                     /locus_tag="VFR20_10055"
     CDS             complement(11211..12383)
                     /locus_tag="VFR20_10055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0230903.1"
                     /translation="MRHVRGQPDVIVIGAGIVGAACAHELAAHGLDVQVIDRATVGAG
                     VTAAGMGHLVVMDESPAEFALSAYSRDLWLALAGQLDARHAFVRCGTLWVATDEEELA
                     VARTRVQVLRAEGIACENLDGAQLREAEPALREGLAGGMVVPDDGVVYAPAVAAWLLA
                     QPVSGRIVTRTGIGVASLDASGARLDDGSRVSAGAILLANGLQAPELLPGLPMQAKKG
                     HLAITDRYPGTVRHQILELGYIKSAHNHSGTSVAFNVQPRPTGQLLIGSSRQFDTLDP
                     AIDPPVLARMLRRAAEYLPALPDLNVIRTWTGFRPASRDGLPLIGPAAQFTHDMDGAP
                     PLWLAVGHEGLGVTTALGTARLLAAQMLDLPLPLDAVPYLPERFAVSVPPSGAANA"
     gene            complement(12389..13351)
                     /locus_tag="VFR20_10060"
     CDS             complement(12389..13351)
                     /locus_tag="VFR20_10060"
                     /EC_number="5.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyproline epimerase"
                     /protein_id="HEU0230904.1"
                     /translation="MRAMKTLTIIDSHTGGEPTRLIMSGGPDLGHGPLSERLRAFRDH
                     FDHFRAGVVNEPRGSDVMVGALLCEPVDAGCSAGVIFFNNVGYLGMCGHGTIGLIVSL
                     AHAGRITAGTHRIETPVGIVDAELHPDGSVTVRNVPAFRHRHAVAVDVPGYGRMHGDI
                     AWGGNWFFLVADHGQALEFSRVDALTEFTWTLRNTLEANGITGQDGALIDHIELFAPS
                     PRADVDSRNFVLCPGKAYDRSPCGTGTSAKVACLAADGHLAPGAVWRQESIIGSVFEG
                     SFAHDSSLPDGRIVPSIRGNAHISAESTLIFNDDDPFAWGIHAS"
     gene            14250..15392
                     /locus_tag="VFR20_10065"
     CDS             14250..15392
                     /locus_tag="VFR20_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU0230905.1"
                     /translation="MNRLTKISALTAVASLTMGLGTAVAASGPIKIGVQAPITGEYAA
                     EGQGIENGVKLLAKQQNAAGGLLGRQIKVIVCDDEGKPAQAAICARKLVNDGVTAVIG
                     TYTSGAALAAAPIYAAANVIQTSDGTSDELTQKGWKTFFRNAPPNSDEALFTAKYLVN
                     VRHDKRIVVLSDHSSYATGLADSVEKAIKADKGNVVAKDFINAGTQDYTAVLTKLKSM
                     NPDVLYFSGYYTDGGLIRAQMEQLGMKAAFVGGDANQNAAFAKIAGNAAKGAIIINVP
                     APQNLPYPEAKKFLADYIAAYGSAPPSIYTFTNADGLRAVFAAIKATKSAETSKLIPW
                     LHDMKKPFDGVTGPFKWDAHGERVGSPMSAFEVQADGSYKTVYPTK"
     gene            15501..>15984
                     /locus_tag="VFR20_10070"
     CDS             15501..>15984
                     /locus_tag="VFR20_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0230906.1"
                     /translation="MDFILQQLINGLTVGGIYSLIALGYTMVYGVLKLINFAHGDLCI
                     LGAFIGLTTLTSGAFGGLSTPVLLVLAFLIAMIIVAVAGTLLDQFAYKPLRKAHRLSA
                     VVSALGASMLIENGIMLIWSPNVQVFPNNLLPNITWQIGGAYLSFVQLLIIICAFLLM
                     A"
BASE COUNT         2813 a         4928 c         5257 g         2986 t
ORIGIN      
        1 ggctccgttg ctcatcgcct tcctgctgtt ccaacgccag ttcgtgcagt cgttcatgcg
       61 ggcgggtata cgctgagcgc ggtcggtggt cgggcaggcg tgcagccgtt catgtgggca
      121 ggcagtcggc gccgacctcg catggcgcta ttcttgtagc gtcgcattcg ttctcccggg
      181 cattggtgcg gccacgtttg ccggtgcttt cactccgctg cggcggcgat ggcctcggct
      241 gggtccggcg ctttgtcggt ggcccgtttc gtttcccgca tcagcagcag gcacggcatg
      301 gatagaagcg tcatccagaa catcaggcgg aagtcgtcga tgtagccgat gaatgcggcc
      361 tgccgggtca ccacctggtt caggccggcc aggccggcgg cggtgcccgg atcgaaaagg
      421 cgcgtcaggc cgctctgatc gagcatgacc gcgcccgcgg tgacgtacgc cgccagcgtg
      481 ctgtgcatgc gcgccgtgtt gcgcaccagc agcgtctgca tggccgagat cccgatgctg
      541 ctgccgatgt tgcgcgagag gctgtagatg gcggttccct ctgggcgcag agcgcggtcg
      601 agcgtggaga acgtcatggt cgtcagcgga accgatgtga agccgatgcc catgccttgt
      661 atgaagccgg gccacacgat atcccagtca ttgatctgca gcgtgtagtt cgacatctgc
      721 cacagtgaag ccgcggtcag gccgaacccc atggccagaa tgagccgact gtccatgcgc
      781 ccgaccaggc gtcccgccag catcatggtc agcatggtgc ccaggccgct gggcgcggtc
      841 accaggcctg tggccatgac cgggtagccc atcaggttct gcagcaacgg cggcagcagg
      901 gcgcgcgtgg cgtagagcag caggcccagc agaaagtaga acgccagtcc cgtcacgaaa
      961 ttccgatccc ggagcagctt cacattgaag aacgagtggc cagtgtccgt gattgtgtgc
     1021 agcacgaaga atgtcaaggc aatgacgccg gccacagcct cggtacagat ctcgatggac
     1081 ccgaaccagt ctttctggtc gccgcgatcc aggatgagtt gcagcatggc aatggcgatg
     1141 gccagcaacg aaaacccgaa ccagtcgaag cgcgccgact gtttattgcc ccgttcgtga
     1201 atgaagtaca gcacacccag gaatgacagg atgcccactg gcagattgat caggaacacc
     1261 cagcgccagc tgaaattgtc ggtcagccac ccgcccaggg tggggcccag caccgggccc
     1321 agcatgacgc ccaggcccca caccgccatg gcccgagcat gccgttccgg cggattgatg
     1381 tccagcatca ccgattgcga cagtggcacc agcgccgcac cgaacatccc ttgcaagagc
     1441 cgggcgcaga cgatttccag caggctcatc gacatgccgc acagcaacga actggccgtg
     1501 aagccggcaa tggaatacag gaaaatcgcc tttcggccat agcggcccga gagccagccc
     1561 gtcacgggtg tggcgatggc tgccgacacg atgtaggacg tgagcaccca catgatctgg
     1621 tcctgcgagg ccgacaggct gcccgccatg tgaggcaggg ccacgttggc gatggtgctg
     1681 tccagtgtct gcatgatggt ggcgagcatg atcgacagcg tgatcatggc acgctggaat
     1741 tccggacgct gttcgctggt ctcgatcacg gtgccgtggt ttctaaaata gtaactatgg
     1801 ttattacatg ggcaaaaaaa atcagtccac cctgtgcagc agcttgtgta gcagccgttc
     1861 cagttcagcg atttccgcag gggtgaggcc gcctagtgcg tgttggacat gctggcggcg
     1921 cgcgggcagg gcgtgctcga cgaaggtgga tcccttcagg gtcagttcca ggaactgttg
     1981 ccgcttgtcg tgttcgtcgg ggatgctatc caccaggccg tggcgttgta gttcgcgcga
     2041 gacgcgtgtg ctgttggttc gggaagagtt catgaacacg ctgagttccg agggtttgag
     2101 acgatggtcg ggactggcat agatgagcag cagggacatc cacaacgtgg tgttcaggcc
     2161 atcgtcccgg agcgaggcgt ccaggtagtc gcccagtttg gtggccacgt gctgcaggag
     2221 gcgggcgacg accgattcac gcagggcgcg ccgtgaagac agccgtcggg acaggtcgtc
     2281 catggcctgt tcgaaggaat cgaatgatga aggggggcga tcgtgttggg gcatagccac
     2341 atagtaacgt aagttactat gtttatggtg gttccagttt gcgccctcgt ctgttcgaga
     2401 cggggcgggg ggctcggccc cgaccggtgt gaaggggcgg cccggtttgg gcacgcgcct
     2461 tggagtgacg tgatcagctc ggcgtggctg tggtgtcagc gctttccgcc aatcgctcgt
     2521 gcagcgccgc cagattcgcc gcggtggatc cgacgccgcc gcatacgacg accgcgatgg
     2581 tcgtggcgcg ccgaagactg tctgcattgg agaacagcgc cgccagcgaa gcaccgcacg
     2641 cgggctcgac gatgagccga tgatcatcca tgaagcgtac gcaggcctcg acagcctgcc
     2701 tgtcctgcac gacaacgctt tccaccgagt ggctgcgcga cagatccagc gcccggtcgc
     2761 aggcgcgccg ggcgcccagc gatgtcgcca cggtggtgat gtcgtccagc gtgacgggtt
     2821 cacccgcttg cagcgactgg gccaggcagt cggcgcccac ggtttccacc gccaccaccg
     2881 gcacgccgcc ccaagaggat ttttgcagcc cttcgatgac gccgcacagc aatccgccgc
     2941 cgcccaccga cagaatgatg gcatccggcc gtggaccctg gcgaaccatt tcgtcgatca
     3001 tggttgaatg tcccgaccag agcaggggat cgtcgaaggg gtgcaacagc acgctgtgtg
     3061 cgtccaccat ggctttcgcc gcctgatgcg cttcgcccca gttcttgcca tggacgacga
     3121 ccaccccttt ttccatggcg atgagggctt tcgcgcgctc ggatgtagac tcgggcacga
     3181 cgaccgtgac aggaaccttc aactgccttc ccgcataggc gacggccaat cccgcgttgc
     3241 cgccggacga cgagatgaat cgttgtgcgc catgttgttt gtgaatcagg caggcgtgac
     3301 ccatgccgcg cgccttgaac gaacccgtcg gctgcagcgc ctcgagcttc agccagacgg
     3361 attttcccgt gccaagcgac agtggacggg attcgatgta cggagtttcg atatgcaagg
     3421 ccatgggtgc cgctcctgaa gagggaaatc gagtgccgtg accaacgggt gccggcaagc
     3481 cattttgccg ccaggcgtgc cgaatcgtca aagcccttca agcgagttca ctgaatgcca
     3541 acgcacaaat atccgcctgg gagtgctatc ttgtcgcttt gcaacgcggt gctgcgtgca
     3601 tagtcattca atttccatca tgaccagaca actcttttac tatcccggca acgccagcat
     3661 ggcgcctcat atcattctcg aagaactggg cgttccccat gagctgtcgt tggtcgaccg
     3721 cgccggaggg gggcacaagt cgccggaata cctcaagctc aatcccaatg gcctgattcc
     3781 cgttctggtc gatggcgaca tggtgttgta cgagtccgcc gcgatctgcc tgtacctgtg
     3841 cgacgcgcat ccgcagtcgg ggcttgcccc ggctttgggc tcgacggagc gcgcccactt
     3901 ctacaagtgg ctgatctgga tgaccaatac cttgcaggcc tcgctgctcg gctatttcta
     3961 tccggagcgc tgggtggacg acggcaatgc cactggcgcc gcgcaggtcc gggctcacgc
     4021 ggaaacgcgt attggcggac tcctggacca gatggaggcc caactggaaa acagtggcgg
     4081 tccatggttc ctggggcagc gtttcacagc ggttgatgcc tacgccgtga tgctgtgccg
     4141 ctggactcgg ggatttgaac gccccgcgcg tgccctgccg cttctcgggg cgtatctgtc
     4201 gcgtgttctg gagcggccgg cggtgcggcg taccttgacg agtgaggggc tgaccgcgcc
     4261 ttgggtatag aggcaatagt tgccgtcgcg ccagattgtt caaaaaggca caataccgat
     4321 atgcaaagcg attcaatttc ccccgccgac tggctcgacg catattgtcg gcacggcagc
     4381 gaaaaaaaaa tagagaaggg caagccatca tcctgatcat tcagacattg gtcgccgcgc
     4441 tcgttgccct ctggggcggc ctggcgctat gggttcaaca gccgggtggc cgcctgtgcg
     4501 ccaaagggtt gatcgtcctc tgggtcctgg cggcgctggc gttcatggcg gtgccgtggc
     4561 ttgcctcgcc tacgtccgtc aaactggctg gccgattgat attccccgcg ctggtggcct
     4621 tggtgctggt gtggtgggcg ggcattcgcc cgtccaacga caagaactgg attccagagg
     4681 tctcccggcc attgcagggc accgtggacg gtgatgtggt cattctggaa aatgtccgca
     4741 actttctctg gcggtcggcc gacgactgca ccccgaggtg ggaaacccgg cactacgatc
     4801 tgaacacgct gcgatcggtg gatttgtcgc tatcgtactg ggcgggcccc gccgtggccc
     4861 atgtcatggt gtccttcggt tttggcgatg gcgggtacgt tgcgttttcg gtcgaaatcc
     4921 gccgcaaggt gggtgacaag ttctctgaaa tcggcggctt ctttcgtcag tacgagctca
     4981 gcatcgtggc cgccgacgag cgcgatatcc tccgcgtgcg caccaatgta cgcggggagg
     5041 acggctatat gtatcgtgtc cacatgccgt tggcggcggc gcggtcgttg ttcctggcgt
     5101 atgtggatcg ggccaaccgg ctggtggcgc ggccccggtt ctacaacacc gtcacggcca
     5161 actgcaccac gctcgtctac cagatgatgg accgcatcgt gccgggcctg cccatgagct
     5221 atcagttgct ggcctcgggt tatctacccg agtatctgta caggctggat gccttggcgg
     5281 gcgcgtcgga cctggctgaa taccgccgcc gtggccgcta tacggaccgc gccctggcga
     5341 acaccgacct tgatcggttt tcgcacgaca tacggcgcgg cgttccaggc atcgacgcag
     5401 catgacggaa atgcgggcgt cagtgccagg cggccaggaa agccatcaaa accttcgcgg
     5461 aattgtcggc ggcgatgtcc aggaatgtat gcatttcgtt ggcaccgggc ccgcccccgg
     5521 ccaggtcact caacgagcgg aacgccagaa acggtacccc gttgctgtag gcgaccgcgg
     5581 cgcaggcggc gctttccatg tccagcacgt tcgcatggaa ggtgtcgaac acgtatcggc
     5641 ggaacgcggc gttgtcgacg aacgccgacc cggacacccc gttgccgccc acaaccagtc
     5701 gcggcgattt ttccaggcaa tggccgcgtt tgtcgcaggc gcccagcgtg acatcgtgca
     5761 ggcccttggc gatggccagc atgtgtgggt cggcgtcgaa ccagaatttg ctttccagtt
     5821 gcgggtgcgc ggccgaacgc acgcgcaccg gccggacaaa catcatgccg taatgcggca
     5881 gatgcagcgt atccttgccg ggtgggggcg tgaacacgcc cggtgccgtc tcgcgcgcca
     5941 tgagcagttc cagatactgg ccccagcggc tggccacggt cacgtcgccg atgtgcagcg
     6001 ccgggttcac gccgccggcg atgccgctga acacgatgtg cgtgatgtga aaacgatcga
     6061 gcgccagttg tgtggtcatc gccgcgttgg tcatactgat gccgctcaag aacagtacca
     6121 ccggtttgcc ctgcagcgag ccggtggtga attcgacgcc attggcggaa aagcggtgcg
     6181 gcgcctggac gtgtttcagc agcagggaca actcgggctg gaatgccgag atcacggcga
     6241 tgcgtggtgt gttgtccagg gtttccgcgg cgcgcaccgt tccgcgtgcg ccgggccagg
     6301 gcagacacag cgtggtcagc gagcagatca acagaagcgt gcgcaggcgg gacaggaaag
     6361 cgggcatggt gaatctccgg acaaggggcg ggtggaatgt cgtggtcccg cagtcatgct
     6421 ggattgtacg gggagtcatt gcgcgcggaa gtcgttgacg cggccgtgtg gagccaccgc
     6481 catcactcac tccggaatcg cgttggtcac atgtcgcgga tccggacgct tcatgaacgc
     6541 ctggcggcgt tgggcctgtt ggttgaccgc cggatgattg agcgtcggtt gaccatatat
     6601 atgattttgg gcaatatttt gcctttccac cccgccttgt ttgaacattt cgcgaagggc
     6661 tggaacgatc gggaagaaac atggcgcggt tgatgttcct cttgctgggt gtcgggtatt
     6721 tgcgcaggcg ctggcgcgta ttgttggtgc tgggtgtcat ctggttgctg gccggtgggc
     6781 tcatcgtcgg cgacgccatg gacggggtgc tctactttcc actcaaattc tttgcctatc
     6841 tgtgcctgct ggaaggcttg tccacgctcg ccatcgcaac gaccggcgtc ggtgggcaac
     6901 gcattctgcg ctatgtgaaa ggtgtcgccg ccgtcgtcgc cgccatcctg gttctggtgg
     6961 actcgcgcca cggccacttt ctactggcca tgatcttcgg cacgctgttc ctgatcgacg
     7021 gggtgatgca gtgcgtggcg gcatatgtgg tgcggttctc ccgttggcgt caaacgcttg
     7081 gcatcggcat cttcgagatc gtgctggcca ttctgttcta tcaaccgtac cccacgcatt
     7141 acgtgggcac attgccgatc gcgctgggct ttttcctgat catggcgggc atcaagctcg
     7201 tcaatctggc ggtgcgcgtc cggcgcatca agacgaaccc cggcctgggc gaatccccca
     7261 ccgacggtct cttgccagag aatctctggg gtgcgcccaa ccagaagccc gtcagcgtgt
     7321 gggatggtcc tccggccgat gatgagtcgg cgctcaccgt gcatgtctgg acgccgacag
     7381 ggtcgtccaa ggcgcaggcg gtgcggtacc ccatcgtcga ccgttatatc gcggcggtgg
     7441 acgtcaacgg ggtcatttcc accggacacg ccgcgctgga gtcgccggag gggatttaca
     7501 tcagcctgta tccggctgaa gaaatcgacc gctcgccgga tcagttcggc cgtctgctgc
     7561 gcgcgacatc cgacaacgat gtgtcgggcc ggttcctgcc caactatggt gtcgaatcac
     7621 gggagtggtg cccgtccacg gtgcgtgtgc gtatccgcaa ttacgatccg gccaagctgg
     7681 ccgcgttctg ggggcaatat cgtcaaaacg cgacctacaa cctcacgcac cggaactgct
     7741 ccagcaccgt ggccaatgcg ctggacgcgg cactcgatgg ctgtgtgggg cgtttgcatc
     7801 gccatgggca tggctggggg ggcttcctgc gtctgctggt cacgcccgaa ctctgggtgg
     7861 ccgcgcaagt gcgcaagcgc gcgcacacca tggcctggac accggggctc acgctggact
     7921 atgcccgtgc catgagcatg ctggcggacc ctcggccgtt cggttggtgg aaaatgttcc
     7981 agcttgccgc ccggcgcatt atgcggctgc gccgccaatg gcgggcagcg gacaggcaag
     8041 tcgggcaggc gccgagcgcg gtcgccggcc ccgaggcctg aatggtttca ctggcgcgac
     8101 tggtgtattt ttgattcagg cgtatacatc tcttggcatt ccacgtgtgc ggtattacgt
     8161 ggttctcgtg ggggctctaa gctgttttgg tctgtgaggc ttgatccagc atggtccgtc
     8221 ccaaatcggc ctggccgcgc tccagcagcg catcggcggc accgcgcagg tcgcgcgcct
     8281 cgcgattctg gctcagcttg aatttgcctt ccaggcaggt gatgtgtatt tcgattccca
     8341 cgatggcctt gagcatggtg tcgatgtatt caggtgggga atccgtcatc ttccagggtt
     8401 tggcttcccg cgcctcgtgg gtccgcgtca gacgggccac gacgccccgc acgtagcgtt
     8461 cgtcgtcccg caccgtgatg cggccgtggg cgtgggcgac gcggtagttc caggtgggga
     8521 ccgacttgtg gttttcgtgc ttgctcgggt accagcttgg cgaaatatag ccgttctccc
     8581 cccggaacac gaccagcacc tcgtccccat cgtgcacgct cgtccatagg ggattggcac
     8641 gcgccacgtg gccatgcagc acgccatgtg aacctgtttt cgcgtcgagt tcaaacggaa
     8701 tgtggttggc gtccaggccg ccatcgccgt gggtgatcaa aacacccaat ggatggtcgg
     8761 tgatgaattc gtgcaggact tcgggtcgcg attcgtcgaa atgggtggga acgtacatgg
     8821 cgggatggtt ccggagaaag gggttccgcc gcgtccgcgg aagaacggga agtatggatt
     8881 atcccgctat tttcagccac tgatcaacgc gcggtactgg ctgggtgtca gccccacggt
     8941 ggctttgaac tggcgcgtga acgcgctgtg gtcgttgtag ccgcatcgcg tggcgacgtc
     9001 ggtgatgctc gcgtctccgg caagcagcgt cgatgcggca tccagccgtg tcttcagcag
     9061 caactgacgc ggcgtcaggt ggaagatctt gcggaaatac cgttcgatct gggcgatcga
     9121 catattggcc agtccggcga gccgggccag ttccaacggc tgggagtagt tgtcctggat
     9181 gtgccgggcg acggtggcca gccgctggta ggccgggtgg cgaccctccg cggcttgcag
     9241 gtcccgtgaa attcccgcca caccgagaat ccggccctgc gcatcccgta gtggcagctt
     9301 gcaggtcagg caccagcctg gttcgcgccc ggggtacagg tgcagttcga gctggtcgat
     9361 cagtttgcca ccgtgcgcca ccacggcttt gtcctgcgcg gtgtagatgc ggccgaagcg
     9421 tgcggggaac acttgttcgg cggtcttgcc cagcagatcg cgcttgtccc tgaaaccaca
     9481 gcgcgaagcc agcgtgcggt tggccagcac gtagcgcgcc tcggtgtcct tcacgaagaa
     9541 cacgacgtcg ggcatggcgt cgaaaacggg ttcgagcagg gcgaagtgcg ccaccatgcc
     9601 atccagcatg gcccgatcgt cggaggacgg ggcgcgatgg ttcatgtggg tgactccggc
     9661 tcgtccagag gccgcatcaa cgtggcgacg cgtgccggcg atagcggcgc gcgggggggc
     9721 gtgcccacgt tttcaccttc gggcccccag ccgaaacaga aggcggccgc cgcgccgcag
     9781 attctgcctt ggcacggccc catgccacag cgcgtctgca atttcgcggc gcgccacgat
     9841 gtctgggccg caacgtcgtg atgggcgaca tcctcgcagc ggcagaagat cgtgtccggc
     9901 gccgccaggg tgcgcagcgc ggggttcagc gcgaaagcct cgtgcatgcg cgccgcgaag
     9961 cggcggtagc gctcgcgcag ggggaacagc ttgcgcgcat cctccgggcg gcccagggcg
    10021 gcgtgcgcgg cgatgcggcc ttcggtggcg gacagttcca tgccgccgac gcccgtgcat
    10081 tcccccgcgg cgaaaacacg atcgaccgat gtcgcctgca acgcgtcgac cgcgacggcc
    10141 ggatcaccgg cgtgcggggt gagccggcaa cccatggcgg ccgccagacg ggtattcggc
    10201 aatagcccga agccgcacgc gagccgatcg catggcacat cgaatgcggc gccccgctgg
    10261 cggattgtca cgcggtccag cgcggcgtca ccgtgcgccg ccgtgatcca ggcatcgcgc
    10321 cagtaggggg ttcgccgcaa ccccagccgc agacgcatcg cctggacgag cttggccggt
    10381 gtcaggagga gtccgcccgc gaagcgcgcg acagcgcgcg ccggagcctg ctcgacgatg
    10441 gcgacgatgc gggcaccgca cgcttgtgcg gtggcggcgg ccgcccacaa cagcggcccg
    10501 ctgccggcca cgatgatgcg ctggccttcc acaggtgtgc cgcccttgat cagggcctgc
    10561 aggccgccgg ctccggtcac gcccggcaat gtccagcccg gaaacggcag gaagcgttcg
    10621 cgcgcgccgg tggcgatgat cacgcggtcg ccggtcaatg tcagactttg ccgggcgttc
    10681 tcgaccatca ggcgcgggcc ggggagcgcc tgcacgatgc gggtgccgct gtggagcgat
    10741 acgttcggcc gatcggccag cgcccggagc agcgttcgcg cctggccgac aggtgggtgg
    10801 tctggtcctt ggcgccagat ctgaccaccc ggtcgcgggt tgtcgtcgat gatgccgacg
    10861 cgggcgccac tgtcggccgc gatccgtgcc gccgacaggc ccgccgggcc cgcgccggcg
    10921 accaggatgt cgaaatgaaa ggtgggctcg gtcatgctga caccgctccg gtgatgactt
    10981 gcatgtcggc cgcgcacaag gtctgacacg ccagccggtg cgcgtggccg tcgatctcga
    11041 cgcggcattc ctggcaaaca cccatgccgc acagcgggcc gcgcagggcg ccccccaccg
    11101 agcgccgggt tgtggcgctg gcgcctccag tggcaagggc gatcgcggcg gcgacggtac
    11161 tgcctgccgt gacggtgaca gggtgtccat caatgaacag gacgatggtc tcaggcattg
    11221 gcggctcccg aaggcgggac ggagacagcg aagcgctctg gcagataggg gacggcgtcc
    11281 agaggaagag gtagatccag catctgcgcg gcgagcagcc gggcggtgcc cagggccgtg
    11341 gtcacgccca atccttcatg gccgacggcc agccatagtg gtggtgcgcc atccatgtca
    11401 tgtgtgaact gcgcggccgg gccgatgagt ggaaggccat cgcgcgaggc gggtcggaag
    11461 cccgtccagg tgcgtatcac gttcaagtcg ggaagggccg gcagatactc ggcggcccgc
    11521 cgcagcatgc gcgccagcac gggcgggtct atggctggat cgagagtgtc gaactggcgc
    11581 gacgagccga tgagcagttg ccccgttggg cgtggctgca cgttgaaggc gaccgaagta
    11641 ccgctatgat tgtgggcgct cttgatgtag cccagttcca ggatctgatg acgcaccgtg
    11701 cccggataac gatcggtgat cgccaggtgc cctttcttgg cctgcatggg cagccctggc
    11761 agcagctcgg gggcctgcag cccattggcg agcaggatgg caccggctga aacgcggctg
    11821 ccgtcatcga ggcgcgcacc cgaggcgtcg aggctggcca cgccgattcc cgtgcgggtg
    11881 acgatgcggc ccgaaactgg ctgtgcgagc agccacgcgg cgaccgcggg cgcatagacc
    11941 accccgtcgt cgggcaccac catgccgccg gccaggcctt cgcgcaacgc gggttcagcc
    12001 tcacgcagtt gcgcgccgtc caggttttcg cacgcaatgc cctccgcccg caacacctgt
    12061 acgcgcgtcc gggccacggc cagctcttct tcgtccgtcg cgacccagag ggtgccacag
    12121 cgcacgaacg cgtgacgcgc atcgagttgt ccggcaagcg ccagccagag atcccgcgag
    12181 taggcgctca gggcgaactc ggcgggtgat tcgtccatga ccaccaggtg ccccatgccg
    12241 gccgccgtga ccccggcgcc caccgtggcg cggtcgatga cctggacatc caggccgtgc
    12301 gcggccagtt cgtgggcaca ggcggcgccc acgatgccgg cgccgatgac gatcacgtcg
    12361 ggttggccgc gaacgtggcg catgcccgtc atgatgcgtg tatgccccaa gcgaaaggat
    12421 cgtcgtcgtt gaagatcagg gtggattccg cgctgatatg cgcattgccg cgtatgctgg
    12481 gcacgatccg cccgtcgggc aggctcgaat cgtgcgcgaa actgccttcg aacacgctgc
    12541 caatgatgct ttcctgccgc cacacggccc cgggtgcgag atggccgtct gccgccagac
    12601 aggccacttt ggcgctggtg ccggtgccac acggcgaccg gtcgtacgcc ttgccggggc
    12661 acagcacgaa attgcggctg tccacgtccg cgcggggaga gggggcgaac agttcgatgt
    12721 ggtcgatcag cgcaccatcc tggccggtga tgccgttggc ctccagcgta ttgcgcagcg
    12781 tccaggtgaa ttctgtcagg gcgtccacgc ggctgaattc cagggcctgg ccgtggtccg
    12841 ccaccaggaa gaaccaattc cccccccagg cgatgtcgcc atgcatgcgg ccgtatcctg
    12901 gcacgtccac ggcaaccgcg tgccggtggc ggaacgcggg cacattgcgc accgtgacgc
    12961 tgccgtcggg atgcagttcg gcatcgacga tgccaaccgg cgtttcgatg cggtgcgtgc
    13021 cggcggtgat gcggccggca tgggccaggg acacgatgag tccgatcgtg ccgtggccgc
    13081 acatgccgag ataaccgaca ttgttgaaga agatgacacc cgccgaacag cccgcatcaa
    13141 ccggttcgca caacagggcg ccgaccatga cgtcggagcc gcgtggttcg ttcaccacgc
    13201 cggcccggaa gtggtcgaag tggtcgcgga atgcgcgcag acgttcactc aaagggccgt
    13261 ggcccaggtc cggcccgccg gacatgatca ggcgggtggg ttcaccgccg gtgtgggaat
    13321 cgatgatggt cagggttttc atggcacgta tcgtaggccc cggagggtgg agtcgtcttg
    13381 catgatctat agggtttgaa tgacgatttt cgcatagttg catgcgaagg gagtactggt
    13441 ttggatcggg cgtgtggcat gaatgttcag acggagcagt ggttgcatga taaagggatc
    13501 atccacggtt gtggacgtgt cggtatgcgc aatgccgcat aagccaccat cggcgaatgg
    13561 tgcggtccgt ggctatcgtg agcgttcgcg acacggcgca cagccccagg ccgcccatgc
    13621 gcgcggcgcg atgactccag aggatcccta ggggaaatcc cgctttcgac gccgcacggg
    13681 gtaggggttt gcactaggtc cacgcgatgg ggctgtgttt tttactatgc cgtaatcctc
    13741 gtcaaggaat agcggcttcg cgcaggtgtc ggcccgtgat ggcgtgcgga tgatatgacg
    13801 tgttttaaaa aaaattagcg tccacaaagg atatgcaagg gtcaaaggaa atggtttccg
    13861 ttgtttcttg tatcagggtt tgtactgaca atcacagtga ctatgcagaa ataatcaact
    13921 gaaacgtgcc aaaatggcaa gacacgctgt cagtgtctat ctatactgtt cagggtggaa
    13981 taggttgcac tcgtaagttt cagaaagtta tacttcgcgc atccttcggg ttgtgcttgt
    14041 tggttgtacc tgtggtgcta cccgaaacga tggagtaacc cggaatgaac ctcactgcca
    14101 tggcaggcga cgcagcacgt tggggctcgc acccatcttc ccgccgagac aagggcaaga
    14161 cgcttcgccc cagggaagat atccatggcg catgtttctc tctacctata taagtagctg
    14221 ttggatcagt tagatcagga gaatccagaa tgaataggct gacaaagatt tccgcgctca
    14281 ccgcggtggc ttcgctgacg atgggtctgg gcacggcggt cgccgccagc ggccccatca
    14341 agatcggcgt gcaggctccc atcacgggcg aatacgcggc cgagggccag ggtatcgaga
    14401 acggcgtgaa actgctcgcc aagcagcaga acgccgccgg cggattgctg ggccgccaga
    14461 tcaaggtaat cgtctgcgac gacgaaggca aaccggccca ggccgccatt tgcgcgcgca
    14521 agctggtcaa tgacggcgtc accgccgtca tcggcaccta caccagtggc gcggccctgg
    14581 cggccgcgcc catctacgcg gccgccaacg tcatccagac ctcggacggc accagcgacg
    14641 aactcaccca gaagggctgg aagacgttct tccgcaacgc gccccccaac agtgacgaag
    14701 ccttgttcac ggccaagtac ctggtcaacg tccggcacga caagcgcatc gtcgtgctca
    14761 gcgaccactc cagctacgcc acgggcctgg ccgacagcgt cgaaaaggcc atcaaggccg
    14821 acaagggcaa tgtcgtcgcc aaggacttca tcaatgccgg cacgcaagac tacaccgccg
    14881 tgctgaccaa gctgaagtcc atgaatcccg atgtcctgta cttctcgggc tactacaccg
    14941 acggcggact catccgcgcg cagatggaac agcttggcat gaaggccgcc ttcgtcggcg
    15001 gtgacgccaa ccagaacgcg gctttcgcca agatcgccgg caacgcggcc aagggtgcga
    15061 tcatcatcaa tgtgcccgct ccgcagaacc tgccgtatcc cgaagccaag aaattcctgg
    15121 ccgactacat cgccgcctat ggctcggcgc ctccgagcat ctacaccttc accaacgccg
    15181 atggtctgcg tgccgtgttc gccgccatca aagccaccaa gagcgccgag acatccaagc
    15241 tcattccgtg gctgcacgac atgaagaagc cgtttgacgg cgtcacgggt cccttcaagt
    15301 gggatgccca tggcgaacgc gtcggtagcc cgatgtcagc ctttgaagtc caggccgacg
    15361 gcagctacaa gacggtttac ccgacgaagt aatcacaccg gcgcacggcg ccgtgcatca
    15421 cccgatgcgc ggcgccgtgt tgttttcgcg cggctcctgg ccgcgcgtgc cgcgatgcgg
    15481 ctcatcacgg aacccgacag atggacttta tccttcagca gttgatcaac gggctgacgg
    15541 tcggcggcat ctattcgttg atcgcgctcg gctacaccat ggtctatggt gtgctgaaac
    15601 tgatcaattt cgcccacggt gacctgtgca tcctgggggc cttcatcggc ctgaccaccc
    15661 tgacctcggg cgccttcggc ggcctgtcca ccccggtgct gctggtcctg gcattcctga
    15721 tcgccatgat catcgtggcg gtcgcgggca cgctgctgga ccagttcgcc tacaagccct
    15781 tgcgcaaggc ccaccgcctg tcggccgtgg tctccgccct gggcgcatcg atgctgatcg
    15841 aaaacggcat catgctgatc tggtccccca acgtgcaggt cttccccaac aacctgctgc
    15901 ccaacatcac ctggcagatc ggcggcgcgt acctcagctt cgtccagttg ctgatcatca
    15961 tctgcgcctt cctgctgatg gccg
//
LOCUS       DASTNK010000135        12292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_106623, whole genome shotgun sequence.
ACCESSION   DASTNK010000135 DASTNK010000000
VERSION     DASTNK010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12292
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106623"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..1653
                     /locus_tag="VFR20_10075"
     CDS             7..1653
                     /locus_tag="VFR20_10075"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEU0230907.1"
                     /translation="MAERESMEYDVVVVGGGPAGLATAIRLKQMAAQGGHDIGVCVLE
                     KGVELGAHILSGAVMDPRALTELIPDWKEKNAPLDTPVTEDRFLFLTEGKSVATPGWL
                     LPECFRNHGNHIVRLGHFTRWLGEQAEAMGVDIFPGFAATEILYSDTGAVRGVATGDM
                     GVARDGSHTAQYQPGMELLARYTVFAEGSRGQLGRQLIERYKLDAGRDPQSYAIGIKE
                     LWEVDPAQSHPGLVMHTSGWPLDADTYGGSFLYHLSDNLVAVGLVVGLDYANPWLSPF
                     EEFQRYKTHPAIRKTFEGGKRIAYGARSLTAGGLLSLPKLCFPGGVLVGCDAGVLNAS
                     RIKGSHSAIKTGALAAEALYEAIVAGREHDELVAYPAAFEKSWLHAELNKSRNFKQWF
                     KKGRTLGTIMTGVEQWLLKGKMPWTLHHSKPDHACLQPAAECARIDYPKPDGTLTFDR
                     LSSVFISNTNHDENEPVHLTLKDPAVPVSVNLAKFGGPEARFCPAGVYEFVKTETGQD
                     RLQINAQNCVHCKTCDIKDPTQNIVWVAPQGGEGPVYNGM"
     gene            complement(1659..2516)
                     /locus_tag="VFR20_10080"
     CDS             complement(1659..2516)
                     /locus_tag="VFR20_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU0230908.1"
                     /translation="MAVTVLLWGMSWIAMKSLASLIGPFDLVVARYAIGYLVLLAYLL
                     VTRQPLRFPPFWLTVGIALFQATALQCFAQFALITGGAGQVVMLAYTMPFWAVLFAWL
                     LLGERPTRHHAAAFVLAALGLLAVIAPWKGMGTLLSSLLALAGGVCWGFGMVLAKMLF
                     LRHKVNVLTVTTWQMLLGALFALPFAVFWPQMPVRWGGELYLGLLYMGVVASALGWAL
                     WMFVVRRVSATVAGMSSLGVPVLTVIFAWLLLGERPGPDELIGIVLIMAGLVAVSLAP
                     PPTRVKAGR"
     gene            complement(2694..4673)
                     /gene="acs"
                     /locus_tag="VFR20_10085"
     CDS             complement(2694..4673)
                     /gene="acs"
                     /locus_tag="VFR20_10085"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEU0230909.1"
                     /translation="MSKEIESILVENRVFPPFPDLVKNARISGMEAYQAMRKEAQQDP
                     DSFWGCLARENLVWTKSFTKVLDASDAPSYKWFGDGELNVSANCLDRHLNTHVADKTA
                     IIFESDDGKVSNVTYKDLYARVCRFANAIAALGYKKGDRSVIYMPMSVEAVVAMLACA
                     RLGVIHSVVFGGFSSKSLHERIVDVGASLVITADEQVRGGKSIPLKPAVDEAFTMGGC
                     DAVRQVIVYKRTGGKVAWTEGRDLWMHEVVAKQPATCEPVPVNAEHPLFILYTSGSTG
                     KPKGVQHSSAGYLLWSLLTVKWTFDSKDTDVFWCTADVGWVTGHSYVTYGPLAAGLTQ
                     VVFEGVPTYPDVGRFWEMIQRHKVSVFYTAPTAIRSLTKACEAAPKYHPREYDLSSLR
                     ILGSVGEPINPEAWMWYYKEVGRERCPVLDTWWQTETGAHMITPLPGVTPMKPGSCTL
                     PLPGIEAAIVDETGEDVEHGKGGFLVMKRPWPGMIRTVWGDPERFKKTYFPPDLKGYY
                     LAGDGAQRDADGYFWIMGRIDDVLNVSGHRLGTMEVESALVAHELVAEAAVVGRPDDT
                     TGEAVVAFVVLKRSLPQGDEANAIAKQLRDWVAKEIGPIAKPRDIRFGENLPKTRSGK
                     IMRRLLRVIAKGETITQDVSTLENPSILDQLSQAV"
     gene            complement(4793..6124)
                     /locus_tag="VFR20_10090"
     CDS             complement(4793..6124)
                     /locus_tag="VFR20_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0230910.1"
                     /translation="MSPTASNAELVSPTPVASPSALNPGVTRREVWSWAMYDFANSGY
                     TTVVLTTVFSAYFVGVVAAHKTWATLALTSALSLSYLMIMLTMPTLGARADARAGKRR
                     LLFTSTVGCVATTALLAGAGPGNVVWGLVFLALSNYCYCIGESVNGAFLPEIARPEAL
                     GRVSGWGWSFGYCGGMLTLGLSLAYVSWAGAHGVPAQVYVPRVMVFTALVFAVAAIPP
                     FLFLRERTLPTRRPAQGMLRRLRAAWIETRGHFPDFRLLLCCGACYQAGIAVVITLSA
                     VYATQAMGFTMGQTMLLVFAVNIAAALGAFSFGYVQDRIGHKRALAITLVGWIVMVLV
                     AYVAVRLWVFWIAATLAGLCMGTSQSAGRAMVGAFAPPDRLAEFYSLWTFAIQLSAVV
                     GPLCYGLITWITSGNQRVALLCTGLFFVAGLLVLSRVDFARGVRVRVTMTT"
     gene            complement(6121..6525)
                     /locus_tag="VFR20_10095"
     CDS             complement(6121..6525)
                     /locus_tag="VFR20_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEU0230911.1"
                     /translation="MKSKPVLTAADVQAILAAAQSHAQVNHWAVSISVVDDGGHPLGF
                     LRLDGAAPISAHIAPAKARTAALARRESKGYEDMINQGRTSFLSVPLDGMLEGGVPIV
                     AGDEVVGAVGVSGVKSPEDAEIARAGIAALVP"
     gene            7010..7618
                     /locus_tag="VFR20_10100"
     CDS             7010..7618
                     /locus_tag="VFR20_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEU0230912.1"
                     /translation="MAGCAANRHTAQTADAIDLHLRTERDNYLQQTKSDPLGAYLSEA
                     QTQNFYSQNLDTQTSYTPSTSRRFKGDQGMANVALDFLGIKYRFGGDTPKTGFDCSGL
                     VAYAAEKSLGLKLPHHAAALARRGEPVKRKDLKKGDLVFFNTRGARYSHVGIYLGHDR
                     FVHAPRTGTRVRIEDMDQSYWKKRYTGARRLAANGPMHTSGK"
     gene            complement(7774..8091)
                     /locus_tag="VFR20_10105"
     CDS             complement(7774..8091)
                     /locus_tag="VFR20_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009456553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HEU0230913.1"
                     /translation="MSEPVRHAVVVGADRLGNIPDLLKGHNIAINHHISGRDTAHQKK
                     APQLPSGTDLLILLTDFLGHNVMKAFRTAAQRAGVRVLVCRRSVCSMKQALAQGGFVE
                     TGV"
     gene            8482..9699
                     /locus_tag="VFR20_10110"
     CDS             8482..9699
                     /locus_tag="VFR20_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0230914.1"
                     /translation="MQSRSLRIVPFALTALTLSLALGTAQAASNEIVLGASVQLTGSS
                     ANTGRYYRDAYKMAIKKINEAGGVKVDGKTYKLALKLYDNQSDVNLSVRQYTQLVSQD
                     KVNFLLGPFASNFTIADSAVSEKYQIPMVEGGGASNQIFERKFKYIFGTLAPASNYFG
                     STIAMLKQVKPEPKSVALLYADDSFDVSVAKGTRPLLKSAGLNTVMDERYSSNTSNFN
                     TLLSQIKAKKVDAVLVAGHETEILNFIRQAKSLNASPNMYSFTVGVPSADFRKALGKD
                     ADYAFGMTAWLPNPALKDQWFGDAAQFAKDYKAKYGYEPDYHAASGISDVETIVKAIE
                     KAGSLNPKKVRDAIAASDFNSLYGKIAFGKDGQISLDQTVVQVQNGAVEPVFNGKKFL
                     TPVKYPMPAWSKR"
     gene            9727..10590
                     /locus_tag="VFR20_10115"
     CDS             9727..10590
                     /locus_tag="VFR20_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0230915.1"
                     /translation="MDLLLQILVNGVLQGGLYAVMALGLALVWGVLNIVNLAHGAFIM
                     LGAYISWHLFTYLHIDPFVGLPITACVMFVLGYAIQRGILNLIARAPMFNTLLITFGM
                     EVVVTYLAQLAFSADFRTINPSYAGDSFTLGVVTIPVVRLAAFGVALLLTFGMWYFLL
                     RSRLGRAIRATAQNLLAARLYGVEPKHLYAITFGLGLALAGAAGGLYGTVSQINPYIG
                     ATLTAKSFAIAIIGGLETPLGVIVGGLFLGIVEALTTLYVGPTFTDVASFGILILVLI
                     LRPNGLLGKVA"
     gene            10587..11537
                     /locus_tag="VFR20_10120"
     CDS             10587..11537
                     /locus_tag="VFR20_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0230916.1"
                     /translation="MKNTSRTILIVVVAIALALLPLYGSDVMIQFGISTLLLAVLAQG
                     WNIIGGYTGYASFGNSVFYGLGGYGVGIAMVQWHLPFSVGLIFGMVLAIVFAVCLGLP
                     VLRLKGHYFAIATLALAQIMAAIVSNIPLAGQNIGLVLPPLNNDRMFYWVSFVLLVFT
                     TLTIWWITRSRFGFGIIAIRENEEGAVVMGVDTTRYKIMAFALAGIFSALAGGIHAYW
                     ITFLDPESAFDITLNVKMIIMAVFGGPGTILGPIVGSFILSAISEILSSEVTSIAGLF
                     FGVVIVVAVVLMPRGLADVIRHFRRSGWHYFIENVRANRL"
     gene            11534..12268
                     /locus_tag="VFR20_10125"
     CDS             11534..12268
                     /locus_tag="VFR20_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0230917.1"
                     /translation="MTAILEARHLTRRFSGLVAVNDVSFTLNQGEILGFIGPNGAGKT
                     TLISLISGTVEPSEGEIYFNDKLINAVPAFKRARLGIGRTFQIMQPFPGLSVLDNVAV
                     GALFGHGGGQPKLAIAREKARECLTFVGLQKHVDHRADELGGPDRKRLELAKALAMQP
                     RVLLCDEVMAGLNLVEIDEVIDVIRKVRGAGISIIVIEHVIKAIKSLSDRIMVLHHGE
                     KIADGQPDEVLANSTVIEAYLGKKRV"
BASE COUNT         2527 a         4039 c         3574 g         2152 t
ORIGIN      
        1 ggaaccatgg ctgaacgcga atcgatggaa tacgacgtcg ttgtcgtcgg aggcggtccc
       61 gccgggctcg caacggcaat acgcctgaaa cagatggccg cccagggcgg gcacgacatc
      121 ggtgtttgcg tcctggaaaa aggtgtcgag ctcggggccc acattctgtc gggcgcggtc
      181 atggacccgc gcgccctgac cgaactcatc ccagactgga aggaaaagaa cgcgccactg
      241 gacaccccgg tcaccgaaga ccgtttcctc ttcctgacgg aagggaagtc cgtcgccaca
      301 ccaggttggc tgttgcccga gtgctttcgc aatcacggca accacattgt gcgcctgggc
      361 cacttcacgc gctggctggg tgagcaggcc gaagcaatgg gggtggacat tttccccggt
      421 ttcgccgcca ccgaaatcct gtattccgac accggcgcgg tcaggggtgt ggccaccggc
      481 gacatgggtg tggcgcgcga cggctcgcac accgcccagt accagccagg catggaactc
      541 ctggcgcgtt acacggtgtt cgccgagggt tcgcgcggac agctcggccg ccagctcatc
      601 gaacgctaca aactcgatgc cgggcgcgac ccgcaaagct acgcgatcgg tatcaaggaa
      661 ctctgggaag tcgaccccgc ccagtcgcac cccggattgg tcatgcacac ctcgggttgg
      721 ccactggacg ccgacacgta tggcggctcg ttcctctacc acctgtcgga caacctggtg
      781 gcggtcggcc tggtcgtggg gctggattac gccaacccct ggctgtcgcc cttcgaggaa
      841 ttccaacgct acaagacgca ccccgccatt cgcaagacat tcgagggcgg caagcgcatc
      901 gcctatggcg cgcggtcgct gaccgcgggc gggttgctgt ccctgcccaa actgtgcttt
      961 ccgggtgggg tgctggttgg ctgcgacgcc ggggtgctca acgcctcgcg catcaagggc
     1021 agccactccg ctatcaagac gggcgccctg gcggccgaag cgctgtacga agccatcgtc
     1081 gccggccgcg aacacgacga actggtcgcc taccccgccg ccttcgaaaa atcgtggctg
     1141 cacgccgaat tgaacaagtc gcgcaatttc aagcaatggt tcaagaaggg ccgcaccctg
     1201 ggcacgatca tgacaggcgt cgaacaatgg ctgctcaagg gcaagatgcc ctggaccctg
     1261 caccacagca aacccgatca cgcctgtctg cagcccgctg ccgaatgcgc ccgcatcgat
     1321 taccccaagc ccgatggcac actcactttc gaccggttga gctcggtgtt catttcaaac
     1381 accaaccatg acgaaaacga acccgtgcac ctgaccctga aagatccggc cgttccggtg
     1441 tcggtcaacc tcgcgaaatt cggcggcccc gaggcgcgct tctgccccgc cggcgtctac
     1501 gagttcgtca agactgaaac cggccaggat cgtctgcaga tcaacgcgca gaactgcgtg
     1561 cactgcaaga cctgcgacat caaagacccg acccagaaca ttgtctgggt ggcaccgcaa
     1621 ggcggcgaag ggccggtcta taacggcatg tagcgaggtc aacgcccggc tttcacgcgg
     1681 gtcggtggcg gcgccaggct caccgccacc agaccggcca tgatcagcac gatgccgatg
     1741 agctcatccg gaccggggcg ctcgcccaac agcagccagg cgaagatgac cgtcagcact
     1801 ggaacgccca ggctggacat gcccgcgacc gtggcgctga cgcggcgcac cacgaacatc
     1861 cagagcgccc accccaatgc ggaggcgacg acacccatgt agagcaggcc gaggtacagt
     1921 tcgccgcccc agcgcaccgg catttgcggc cagaacacag caaagggcaa cgcgaacagc
     1981 gcccccagca acatctgcca ggtggtgacc gtcaacacat tcaccttgtg gcgcaggaat
     2041 agcatcttgg cgagcaccat gccaaatccc cagcagacgc cgccagccaa cgccaaaagc
     2101 gaactgagca gcgtgcccat gcccttccat ggggcaatca cggcgagcag gcccagggcc
     2161 gccaggacaa aggccgccgc atgatgccgc gtcggacgct cgcccagcaa cagccaggcg
     2221 aacagcacgg cccagaacgg catggtatag gccagcatca ctacctgccc ggccccgccc
     2281 gtgatcaagg cgaactgtgc gaagcactga agcgccgtgg cctggaacaa ggcgatgccc
     2341 acggtcagcc agaacggggg aaaccgcagc ggctgccggg tgaccaacag ataagccagc
     2401 agcacgaggt agccgatggc gtaacgcgcc acgaccagat cgaacggccc gatcagactc
     2461 gcaagcgatt tcatggcgat ccagctcatg ccccagagca gaaccgtcac ggccatcagc
     2521 gccagcgcgc cgcgatcgga ttgttgtgat ttcaagatgt gtgaatattc ccacgttgca
     2581 ccggcttacg ccgggcttgc cgccactcgg cgccccgcca accattcgga ttcgccacga
     2641 aaaaaaatgc gaggcgctgt caccccgcat gctggttcaa cagaacgaac gcgtcagaca
     2701 gcctgcgaca actgatccag aatggacggg ttctcaaggg tcgagacgtc ctgcgtgatg
     2761 gtctcgccct tggcgatgac acgcagcagc cggcgcatga tcttgcccga acgtgtcttg
     2821 ggcaggttct cgccaaagcg gatgtcacgc ggcttggcga tgggcccgat ctctttggcc
     2881 acccagtcgc gcagttgctt ggcgatggca ttcgcctcgt cgccctgcgg cagcgatcgc
     2941 ttgagcacca cgaacgccac gaccgcctcg cccgtggtgt cgtcgggacg gccgaccacc
     3001 gccgcttccg ccacgagctc gtgcgcgacc agcgccgact cgacctccat ggtgcccagc
     3061 cggtgacccg acacgttgag cacgtcgtcg atgcggccca tgatccagaa atagccgtcg
     3121 gcgtcgcgct gcgctccgtc tcccgccaga taatagcctt tcaggtcggg tgggaagtag
     3181 gttttcttga agcgttcggg gtcgccccag acggtgcgga tcatgccggg ccacgggcgc
     3241 ttcatgacca ggaagccacc cttgccatgt tcgacgtctt caccggtctc atccacgatg
     3301 gcggcctcga tgcctggcaa tggcagcgtg cacgagcccg gtttcatggg ggtgacgccg
     3361 ggcagcgggg tgatcatgtg cgcaccggtt tcagtctgcc accaggtgtc gagaaccggg
     3421 cagcgctcac gccccacttc tttgtagtac cacatccagg cctcggggtt gatgggctcg
     3481 cccaccgacc ccaggatgcg caggctgctc aggtcgtatt cgcgcggatg gtatttgggc
     3541 gcggcttcgc acgccttggt cagcgaacga atggccgtgg gcgccgtata gaacaccgac
     3601 accttgtggc gctggatcat ttcccagaaa cggccaacat ccggataagt gggaaccccc
     3661 tcgaacacca cctgcgtcag gccggcggcc agcgggccat acgtgacata gctgtgaccc
     3721 gtgacccagc ccacgtctgc cgtgcaccag aagacgtcgg tgtccttgga gtcgaacgtc
     3781 catttgaccg tcagtagcga ccatagcagg tagcccgccg aggagtgctg cacgcctttc
     3841 ggcttgccgg tggaacccga ggtgtacaga atgaagagcg gatgctcggc attgactggc
     3901 accggttcgc aggtggcggg ctgcttggcg acgacctcgt gcatccagag gtcgcgccct
     3961 tccgtccagg ccactttgcc gccggtgcgc ttgtagacga tgacctggcg cacggcatcg
     4021 cagccaccca tggtgaaggc ctcgtcgacg gcgggcttga gcggaatgct cttgccgccg
     4081 cgcacctgct cgtcggccgt gatgaccaac gaggcgccga cgtcgacgat gcgctcgtgc
     4141 aggctcttgg acgaaaatcc cccgaacacc accgaatgaa tgacccccag gcgcgcgcac
     4201 gccagcatgg ccacgacggc ctccaccgac atgggcatgt agatgaccga gcggtcacct
     4261 ttcttgtagc ccagcgccgc gatggcgttc gcgaatcggc agacacgcgc gtacaggtct
     4321 ttgtaggtga cgttcgacac cttgccatcg tcggactcaa agatgatggc cgttttgtcg
     4381 gccacgtgtg tgttcaggtg acgatcaaga caattggccg agacgttcag ttcgccgtcg
     4441 ccaaaccatt tgtaggatgg cgcatcggaa gcgtccagca ctttggtgaa cgatttggtc
     4501 cagaccaggt tctcgcgcgc caggcacccc cagaaactat cgggatcttg ctgggcttcc
     4561 ttgcgcatgg cctggtaggc ctccatgccg gatatgcggg cgttcttgac tagatcgggg
     4621 aacggcggaa agacgcgatt ctccaccaaa atcgattcaa tctctttcga catgatgtct
     4681 tgctccttgc ttgatggtaa ttggtatgtc tttcaatcca atccggccgc tctgccgaaa
     4741 aacctgcacg cgcctttgca cgccggtatc gcacctggcg caccccggcg actcaggtcg
     4801 tcatagtaac gcgcaccctc acgccccgcg caaaatccac ccttgacaga accagcagcc
     4861 cggcaacgaa gaacagcccg gtgcacaaga gcgccacacg ctgattaccc gatgtaatcc
     4921 aggtgatgag gccatagcac aatgggccga cgacggcaga cagctggatg gcgaacgtcc
     4981 aaagcgaata gaattccgca aggcggtcgg gcggcgcgaa cgcccccacc atggcccggc
     5041 ccgcgctctg actggtgccc atgcacagac cggccagcgt ggcggcgatc cagaacaccc
     5101 acaaccggac ggccacataa gcaaccagga ccatgacgat ccagccgacc agcgtgatgg
     5161 ccagggcccg cttgtggccg atgcggtcct gcacataccc gaacgagaac gcacccagcg
     5221 cggccgcgat gttcacggcg aacaccagca gcatcgtctg ccccatcgtg aagcccatgg
     5281 cctgcgtcgc gtacaccgcc gacagcgtga tcaccacggc gatgccagcc tggtaacacg
     5341 ccccgcagca caggagcagg cgaaaatcgg ggaagtgccc gcgcgtttcg atccaggcgg
     5401 cccgcaggcg gcgcaacatg ccctgcgcgg ggcgtcgcgt ggggagggtg cgctcgcgca
     5461 gaaacagaaa agggggaatg gccgcgaccg cgaaaaccag ggcggtgaag accatgacac
     5521 gcggcacgta gacttgggcg ggcacaccgt gcgcccccgc ccaggacaca taagccaacg
     5581 ataggccaag cgtcagcatg ccgccgcaat aaccgaagct ccagccccag cccgagaccc
     5641 gccccagcgc ctcgggacgg gcgatttcgg gcaggaaggc cccattgact gattcgccga
     5701 tgcaataaca atagttggac agcgccagga agaccagccc ccacaccaca ttgcccggcc
     5761 ccgcgcccgc cagcagcgcc gtggtggcca cgcagcccac tgtgctggtg aaaagcagcc
     5821 gacgcttgcc cgcgcgcgca tcggcgcgcg cgcccagcgt cggcatggtg agcatgatca
     5881 tcagatacga caacgacagc gccgacgtca gggccagggt ggcccaggtt ttgtgcgccg
     5941 ccaccacgcc gacgaaatag gcgctgaaca cggtggtgag caccaccgtg gtgtagccgg
     6001 aattggcgaa atcgtacatg gcccacgacc agacctcgcg acgcgtgacg ccggggttca
     6061 gggcggaggg gctggcgacg ggagttggcg acacaagttc cgcattggat gcggtcggac
     6121 tcatgggacg agcgcggcaa tgcccgcccg ggcgatctcg gcatcttcgg gtgatttgac
     6181 gcccgataca cccaccgccc ccacgacctc gtccccggcc acgatgggca ccccgccctc
     6241 gagcatgccg tcgagcggca ccgacagaaa ggacgtccgt ccctggttga tcatgtcttc
     6301 gtaacccttg gattcacgcc gggcgagcgc cgcggtgcgc gccttggccg gcgcgatatg
     6361 cgccgagatc ggtgccgcgc cgtcgagacg gagaaaacca aggggatggc cgccgtcatc
     6421 gacgaccgag atactgaccg cccagtgatt gacctgggcg tggctctggg cggcggccag
     6481 aatggcttgc acgtcggccg ccgtcaaaac cggtttggac ttcatagtgt ttccttgatg
     6541 taatgccggg ctgtggaaca tccgatggcg gagagtgcgc ccaagcggat tttcaccaat
     6601 gcggaacaca acagcattcc gctgaaattt tgttacaaaa cacagcttcg cagcctgagg
     6661 cgtttcgcac ataggctatc cgctggcgcg catggtgtaa agcggaaaaa ctcgctttac
     6721 gcctcaaaga tatttggtta aactcggccg gtattttggg ttccggttca aggggccatg
     6781 gctcgcgtgc gggcttttcc gttaccaaca attcctgatt ccgcgttctg tatttgcata
     6841 ggcccggagg ctatgcgcca agcccgagtc aggaccagca cacaaccaga actcgacaat
     6901 gccgccacat cgactccctg cacagcccct acccgatccc acccagaact ccctccaaca
     6961 cgcactatgg cgcgcgacca agctactgat actggcctcg tcccttgcac tggccggttg
     7021 cgccgccaac cgccacaccg cccagaccgc cgacgcgatc gatctccacc tccgcactga
     7081 acgcgacaac tacctgcagc agaccaaatc cgacccgttg ggtgcctatt tgtcggaagc
     7141 gcagacccag aatttctact cccagaatct cgacacccaa acctcctata cgcccagcac
     7201 ctcccggcgc ttcaagggtg accagggcat ggccaacgtg gcgctggatt tcctgggcat
     7261 caaataccgc ttcggcggcg acacgcccaa aaccgggttc gactgcagcg ggctggtggc
     7321 ctacgccgcg gagaaatcgc tgggcctgaa actgccgcac cacgccgccg cgctggcgcg
     7381 acgcggggaa ccggtcaaac gcaaagatct caagaaaggg gatctggtct tcttcaatac
     7441 ccgcggcgcg cgctactcgc acgtgggcat ttatctgggc cacgacagat tcgtccacgc
     7501 gccgcgcacc ggaaccaggg tacgtatcga agacatggac caatcctatt ggaaaaagcg
     7561 ctacaccggc gcacgccgac tggcggccaa cggccccatg cacacctcgg gtaaatagcc
     7621 aaaccttctc tgcgcgccgc ggcggtcaac acgcaccccg cgtggcggcg ccggcgcgaa
     7681 ctgcggcaca cgcgtcaccg cgcagcgccg tggcgcaaat agaacaaaca aaaacaggcc
     7741 gcccccctac aggctggccg cccactctgc caatcaaacc ccagtttcca cgaagccacc
     7801 ctgcgcaagc gcctgcttca tgctgcacac cgaccgtcgg cacaccagca cgcgcacgcc
     7861 cgcgcgctgg gccgccgtcc gaaacgcctt catgacgtta tgccccagga aatcggtcaa
     7921 caggatcaac agatcggtgc ccgatggcaa ttggggcgct ttcttctgat gcgccgtgtc
     7981 gcgtccactg atgtgatggt tgatggcgat gttatgccct ttgagcagat cgggaatatt
     8041 gcccagtctg tccgcaccca ccacgacggc atgccgcact ggttctgaca tatagacctc
     8101 gcttgcgatt gttgaagttt ggtggagccc gttcaagccc ggtaacgctc gcacatggat
     8161 aagcttgatg atgtttttta ataataatga ttcttattat caaaatcaat ttttattctt
     8221 tcgaaatgct cctaaaaact ttcaataacc gccgccacac caacctgtcc cagcgcgggc
     8281 cgccacatct tgaccagaca caatttcagc gaaagttcag ctattgccgt gcaacaagca
     8341 cagaaatatc gggatttacc cgaaccgcca tgatcaagga caaaaaattc agttcacagt
     8401 caaccgaatt ttggacaatg cgaacaattc ctccattcaa cagacaatgg ggaaacggac
     8461 ttctcaaagg aggttttact catgcaatcc agatcactac gcatcgtccc attcgccctg
     8521 acggcactca ctctgagcct cgctctgggc accgcccagg ccgcatcgaa cgaaatcgtc
     8581 cttggcgcgt cggtgcagct caccggatcc agcgcgaaca ccggccgtta ctaccgcgac
     8641 gcctacaaga tggcgatcaa gaagatcaac gaagccggcg gcgtcaaggt ggacgggaag
     8701 acctataaac tggccctgaa actctatgac aaccagtccg acgtgaacct gagcgtgcgc
     8761 caatacaccc agctcgtgtc tcaggacaag gtcaacttcc tgctcgggcc cttcgccagc
     8821 aacttcacca tcgccgactc ggccgtgtcc gagaaatacc agatcccgat ggtcgaaggc
     8881 ggtggcgcat ccaaccagat cttcgaacgc aagttcaagt acatctttgg cacgctggca
     8941 cccgcgagca actacttcgg cagcaccatc gccatgctga aacaggtcaa acccgaaccc
     9001 aagtcggtcg cgttgctgta tgccgacgac tcgttcgatg tgtccgttgc gaaaggcacc
     9061 cggccgctgt tgaaatccgc cggcctgaac acggtgatgg acgaacgtta cagttcgaac
     9121 accagcaatt tcaacaccct gctgtcgcag atcaaggcca agaaggtgga tgccgtactg
     9181 gtggccggcc atgaaaccga gatcctgaac ttcatccgcc aggcgaaaag cctgaacgcc
     9241 tcgcccaata tgtattcgtt cacggtgggc gtgccgagcg cggacttccg caaggcgctg
     9301 ggcaaggatg ccgactacgc cttcggcatg acggcctggc tgcccaaccc ggcgctcaag
     9361 gaccagtggt tcggtgacgc cgcgcagttc gccaaagact acaaggccaa atacggctac
     9421 gagcctgatt accacgccgc ctcgggcatt tccgacgtgg aaaccatcgt caaggccatc
     9481 gagaaagccg gcagcctgaa tccgaaaaag gtgcgcgacg ccatcgccgc cagcgacttc
     9541 aacagcctgt acggcaagat cgccttcggc aaggacggcc agatctcgct cgaccagacc
     9601 gtggtgcagg tgcagaacgg tgccgtggaa cccgtcttca acggcaagaa gttcctgacg
     9661 cccgtgaaat acccgatgcc tgcctggtcc aagcgctagg ccgtaccgcc cacacaagga
     9721 gtcaccttgg atttactgct gcaaatcctg gtcaacggag tcctgcaggg ggggctgtat
     9781 gccgtcatgg cactcggcct cgccctggtg tggggtgtgt tgaacattgt gaatctggcc
     9841 cacggcgcat tcatcatgct gggggcctat atttcctggc acttgttcac ctacctgcac
     9901 atcgacccgt tcgtcggcct acccatcacg gcctgcgtga tgttcgtgct cggatacgcg
     9961 atacagcgcg gcatcctgaa cctgatcgca cgcgcgccca tgttcaacac cttgctgatc
    10021 acattcggca tggaagtcgt cgtcacctac ttggcgcagt tggcgttctc ggctgacttc
    10081 cgcaccatca acccgtccta cgcgggggac agcttcacgc tcggcgtcgt caccattccc
    10141 gtggtgcggc tggccgcgtt cggtgtcgcg ctgctcctga cgttcggcat gtggtatttc
    10201 ctgttgcgat cgcgactggg acgcgccatt cgcgcgaccg cccagaatct gctggcggcg
    10261 cggctgtacg gcgtcgaacc caagcatttg tacgcgatca cgttcgggct gggcctggca
    10321 ttggccggtg cggccggtgg cctgtatgga acggtgtcgc agatcaatcc gtacatcggc
    10381 gccacactta cggcgaaatc gttcgccatc gccatcatcg gcggcctgga aaccccgctg
    10441 ggtgtcattg tcggcggcct gttcctgggc atcgtggaag cactcaccac actgtacgtc
    10501 ggccccacgt tcaccgatgt ggccagtttc ggcatcctga tactggtgct catattgcgg
    10561 cctaacggcc ttcttgggaa agttgcatga agaacacctc acgcaccatc ctgatcgtgg
    10621 tcgtggccat cgccctggca ttgctgccgc tgtatggctc cgacgtcatg atccaattcg
    10681 gcatcagcac cctgctgctg gccgtgctgg cgcaaggctg gaacatcatc ggtggctaca
    10741 ccggctacgc gtctttcggc aattccgtct tctacgggct gggcggttac ggcgtgggca
    10801 tcgccatggt gcaatggcac ttgccgtttt ccgtgggcct gatcttcggc atggttctgg
    10861 ccattgtctt cgcagtctgc ctggggttgc ccgtgctgcg gctcaaaggc cactacttcg
    10921 ccatcgccac actggccctg gcgcagatca tggccgccat cgtgtccaac atccccctgg
    10981 ctggccagaa tatcggcctg gtcctgccac cgctgaacaa cgaccggatg ttctactggg
    11041 tatcgttcgt cctgctggtg ttcaccaccc tgaccatctg gtggatcacc cgcagccggt
    11101 tcggattcgg catcatcgcg attcgcgaga acgaagaagg cgcggtcgtc atgggtgtgg
    11161 acaccacacg ctacaaaatc atggcgttcg cattggcggg catattcagc gcattggccg
    11221 gcggcatcca cgcctattgg atcaccttcc tcgatcccga aagcgcgttc gatatcactc
    11281 tcaatgtcaa gatgatcatc atggccgtgt tcggcggtcc aggcaccatc ctgggtccca
    11341 tcgtcggttc gttcatcctg tcggccatct cggagatcct ttccagcgaa gtcaccagca
    11401 tcgccggcct gttcttcggc gtggtcatcg tggtggccgt ggtcctgatg ccacgcggcc
    11461 tggcggatgt catccgtcac ttccgccgtt cgggctggca ttatttcatt gaaaacgttc
    11521 gggccaatcg cttatgaccg ccatcctcga agcacgccac ctgacgcgcc gattctccgg
    11581 gctggtggcg gtgaatgacg tcagtttcac cctcaaccag ggagagatcc tgggtttcat
    11641 cggccccaac ggggcgggca aaaccacact gatcagcctg atcagcggta cggtcgaacc
    11701 cagcgagggg gaaatctact tcaacgacaa gctcatcaac gccgtgccgg ccttcaagcg
    11761 cgcccgtctg ggcatcggcc gcacgttcca gatcatgcag cccttcccgg gcctttccgt
    11821 gctcgacaac gttgccgtgg gtgccctgtt cgggcatggc ggcggccagc ccaaactggc
    11881 catcgcacgc gaaaaagcac gcgaatgcct gaccttcgtc gggctgcaaa aacacgtgga
    11941 ccaccgcgcc gacgagctcg gcggcccgga ccgcaagcgg ctcgagctgg ccaaggccct
    12001 ggccatgcag ccgcgcgtcc tgctgtgcga cgaagtcatg gccgggctca atctggttga
    12061 aatcgacgag gtcatcgacg tgattcgcaa agtgcgcggc gccggcatca gcatcatcgt
    12121 catcgaacac gtcatcaaag ccatcaaaag tctgtcggac cgcatcatgg tgctgcacca
    12181 cggcgaaaaa atcgccgatg gacagcccga cgaagtgctg gccaattcca cagtgatcga
    12241 agcgtatcta gggaagaaaa gagtatgacc agcgcaagcc ccaaccggag cc
//
LOCUS       DASTNK010000136         7731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1590083, whole genome shotgun sequence.
ACCESSION   DASTNK010000136 DASTNK010000000
VERSION     DASTNK010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7731
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590083"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VFR20_10130"
     CDS             complement(<1..400)
                     /locus_tag="VFR20_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0230918.1"
                     /translation="MTTPAKNTICLWYDGDAEEAARFYAKTFPDSSIGAVHRAPGDYP
                     SGAQGDVLTVEFTVMGIPCLGLNGGPTFKHCEAFSFQVATVDQAETDRYWNAIVDNGG
                     QESACGWCKDKWGVSWQITPVVLTEGYTNPD"
     gene            complement(781..1188)
                     /locus_tag="VFR20_10135"
     CDS             complement(781..1188)
                     /locus_tag="VFR20_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU0230919.1"
                     /translation="MLRYLLDTNIVSYVLKRRPVEVLTIFNANASRMAISSITLAELF
                     HGAEKSSRVGENLSVIEDFCSRLDVLPYGVKAAQHYGAIRAALEKQGQPIGVNDLHIA
                     GHARSEGLVLVTNNVSEFARVPALEVENWMSAS"
     gene            complement(1188..1421)
                     /gene="vapB"
                     /locus_tag="VFR20_10140"
     CDS             complement(1188..1421)
                     /gene="vapB"
                     /locus_tag="VFR20_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEU0230920.1"
                     /translation="MSVGTVFINNRTQAVRLPVDVRLPEGVHKVEVRARGNERIIAPV
                     GQTWDSFFLDGPAVSDDFMSERGTQHQPERESL"
     gene            complement(1689..2789)
                     /locus_tag="VFR20_10145"
     CDS             complement(1689..2789)
                     /locus_tag="VFR20_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEU0230921.1"
                     /translation="MTNFSRARHTFHPAWGIAVFLLLAVVGLFYVKWSPYYDRAFVAA
                     QMHSIGQSMLTAGADTVPAPSLRAALDYAWAYGRSIWQALLLGLLLGSAMQALLPAHL
                     VRKWLGAASNRSVLAATLLSVPSMMCTCCSAPVVTGLRRCKAASGSTVAYWLGNTMLN
                     PATLVFMGFVLGWKWVGLRLVLGLVLVLGLGWLLNRMNRVSGPASTGMPHSSDSAAQM
                     AQQPDDAEPRLDLAQALQCWARAFVGMALRLLPEYLVLVLLLGATRAWLFPALGVGVD
                     DSLLWIVGAAVAGMLFVIPTAGEVPIVQAMLSLGMASGPAAALLLTLPPVSLPSLAMV
                     AKSFQARELFVVIAGVVMMGVLAGGAAIALGY"
     gene            2994..3692
                     /locus_tag="VFR20_10150"
     CDS             2994..3692
                     /locus_tag="VFR20_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0230922.1"
                     /translation="MSTSYDVKLLRKQEIAEGTLEFTLQKPPGFEYRAGQFGDLVLPA
                     STGLDESNNKHGFSFVSAPFEETLRMATRMRATSRYKQAAAGVPEGTMVKLLALWGDF
                     TLQKNDKIPAVFLIGGIGITPVRSIIAQATHEKTRHKITLIYSNRTPAQAAYADELAH
                     LAEINPNFRYVPVYSDTDGFVTADTVRRHVPDVAAPRYYLSGPEGMVKAMRKILAEVG
                     ADEDNIKTEEFEGY"
     gene            3872..4141
                     /locus_tag="VFR20_10155"
     CDS             3872..4141
                     /locus_tag="VFR20_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230923.1"
                     /translation="MDNLMDASTAMDYERHVLDFTDRAKSALSKEALLSICKHYQSTR
                     GFFAKREFVAVFRRPESVAVVWRQWFTKQPGEFVAEMVLVEVNGK"
     gene            4314..5960
                     /locus_tag="VFR20_10160"
     CDS             4314..5960
                     /locus_tag="VFR20_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEU0230924.1"
                     /translation="MTSIFDQDLPPTAANFAPLTPLLYLERAAEVYPQRLAVVHGLGD
                     TAIRRDWADVYRRCRQLASALAGQGIGRGDTVAVMLPNIPAMVEAHFGIPMCGAVLNT
                     LNTRLDAATLAFMLDHGEARAVLVDREFSPVMREALALRQRTEPVLLIDVDDPLYDGP
                     GEAIGTLGYEALLDQGDPDYAWLMPVSEWDAISLNYTSGTTGNPKGVVYHHRGAALNA
                     VSNILEWDMPKHAIYLWTLPMFHCNGWCFPWTVAARAGVNVCLRRVDVAAMVDRMVEH
                     GVTHYCGAPIVHSMLVNAPQALRDRIPSGISAMVAGAAPPASMIEGMESLGIDLTHVY
                     GLTEVYGPATVCAKHADWDLLNIGERARLNARQGVRYHLERSATVLNPATMQPVPADG
                     QTMGEIMFQGNICMKGYLKNPQATQEAFAGGWFHSGDLAVVDPDGYIKIKDRSKDIII
                     SGGENISSIEVEDVLYRHPAVLAAAVVARPDDRWGETPCAFVELKADASVSAEDIIAH
                     CKAHLAGFKMPRRVVFGELPKTSTGKIQKFELRQRAAKAP"
     gene            6030..6920
                     /locus_tag="VFR20_10165"
     CDS             6030..6920
                     /locus_tag="VFR20_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230925.1"
                     /translation="MMTTFKKAVSLASALLFAGLFFRTADASQVHETARKFIIYEYVP
                     YSTHIADGEVVTEPSFKSYLESLGIHRIDVIYRNRFMTDGRPDKQKIEDIAKSALAAP
                     GIPASFDTEFGQRFKPETVIPRVSEILRIFHHYNTVIPAGVYATAPQNTFAWKPNTPA
                     RFDALNPRYASVARQVDFLSPVLYNYNGGDTARWRLNADYNMQAAKKYHTGKPIIPYI
                     NIAVVLKTSKRDKRAPILVRMLTEAQMHARLQALYDLGASGCIVWASTRFRTADGKTP
                     VFDRNSGWGKALADFAQAHG"
     gene            complement(6948..>7731)
                     /locus_tag="VFR20_10170"
     CDS             complement(6948..>7731)
                     /locus_tag="VFR20_10170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU0230926.1"
                     /translation="HGAHLLQSCAVRSIETQAGRVCGVVTEQGPIQCDQVVLAGGSWS
                     RLFAGNLGISIPQLKVLATAARVDTLDGRIPALPVAADNFAFRRRADGGYTIARSNAN
                     IAQITTDNFRLFKEFAPALAKGWRELRVRAGKQLFKDLRVPKRWSNQDTTPFEQTRIL
                     DPDPAPGLNHEAVRNLSHAFPGFAQCRISHEWAGMIDVTPDAVPIIGQAGQIDGLFIA
                     TGFSGHGFGLGPAAGELIADLVRGVAPRVDPSPFRFGRFTKK"
BASE COUNT         1616 a         2328 c         2283 g         1504 t
ORIGIN      
        1 ggtcggggtt ggtgtagccc tcggtcaaga ccactggtgt gatctgccag gagacgcccc
       61 atttgtcctt gcaccagccg cacgcgctct cctgtccacc gttgtccacg atggcgttcc
      121 agtagcggtc ggtttccgcc tgatcgacgg tggcgacctg aaacgaaaat gcctcgcagt
      181 gcttgaatgt cggtccgccg ttgagtccga ggcagggaat ccccatgacg gtgaactcca
      241 ccgtcaggac atcgccctgt gcgccggatg gatagtcgcc cggcgcccgg tgcaccgcgc
      301 cgatggatga atcggggaag gtcttggcgt agaagcgcgc ggcctcttcg gcgtcgccgt
      361 cgtaccagag gcagatggtg ttcttggctg gtgtggtcat gaaatacctc cggtgacttg
      421 agggtttgtg cggttccggc gctgcttcca cgccggattg catgattcgg cttacttgtg
      481 gacaatgtac actttacgcg cgttgaagtc gaaagtgaac aaaaagtgaa tggtgtccag
      541 gtaagaatcc gtaagaacgc tgatgtcgat tacgggcccc tgccagaacc gccgtgcggg
      601 acgtttctca aacgcaaagc ggaggatttg atcaatacaa atcagaagct gttttttgat
      661 ttacagctta tattttgtga attggaaata cagcgtgaga tttgtacgac ggagttcgcc
      721 gtccgtactg ccgagcggtt accggcgctt cttggatggg tcatccgccg cttgcgatcg
      781 ttaactcgca ctcatccaat tctccacttc aagcgctggc acgcgtgcga attctgatac
      841 gttgttcgtg accagcacga ggccttcgct gcgtgcgtgg ccggcaatat gcaggtcgtt
      901 caccccgatg ggttggccct gcttttcgag agcggctcga atcgcgccgt agtgctgggc
      961 tgccttgacg ccatagggca gcacatccag gcggctgcag aagtcttcga tgacagacag
     1021 gttctcgccg acgcggctgc ttttctctgc gccatgaaaa agctcggcca aggtgatgga
     1081 cgagatcgcc atgcggctgg cattggcgtt gaagatggtc agcacctcaa ccggacgccg
     1141 cttcaagaca tagctgacga tgttggtatc aaggagatag cgcagcatca gagcgattcc
     1201 cgctccggct ggtgctgggt gccgcgttcc gacatgaaat cgtcactgac ggcggggccg
     1261 tccagaaaaa agctgtccca ggtctgaccg accggtgcga tgatgcgctc attccccctg
     1321 gcgcgcactt ccaccttgtg cacgccctct ggcagacgca cgtccaccgg aagccggaca
     1381 gcttgtgtgc ggttgttgat gaagacggtg ccaacggaca tattgctgcc ccccgatgtt
     1441 ggaatgacgt atatggcaat agtatatagt attgtggatc caaatcaaga atgccgtctc
     1501 gctttgtgga aaattgcaag ctaaacgttc aaaggcagcc gtttttctgg cgggcgacga
     1561 ttcctctttc atgaccggag cgagtcttgt gctggatggc ggctggacta cccgttgatt
     1621 gcattgatgg aaggctggcg gcgcttgcca gtcgaggtag cagacctcga ctagaacggc
     1681 ggtagacctc aataccccag cgcgatggcc gcaccgccag ccagaacccc catcatcacc
     1741 acacccgcga tcacaacaaa aagttcgcgc gcttgaaagg atttcgccac catggccagg
     1801 gatggcaggc tgacgggcgg caatgtgaga agcagcgctg cggccggccc gctggccatg
     1861 cccaatgaca gcatggcctg aacaatgggc acttcaccgg ctgtgggaat gacgaacagc
     1921 atgcctgcga cggcagcgcc aacaatccac aacaggctgt cgtcaacgcc aacgcccagt
     1981 gccggaaaca gccaggcgcg tgttgcgccc agcagcagga ccaggacaag gtattcaggc
     2041 agcaagcgca atgccatccc cacgaaggca cgtgcccagc attgcaatgc ctgtgccagg
     2101 tccagccggg gctccgcgtc atccggctgt tgcgccatct gcgccgctga atcagaactg
     2161 tgtggcattc cggttgaggc cgggccagac acgcgattca tgcgattgag cagccaaccc
     2221 agacccaata ccagaacgag ccccaacacc aggcgcaagc cgacccactt ccaacccagg
     2281 acaaacccca tgaacacaag cgttgcgggg ttcaacatgg tattgcccag ccagtacgcc
     2341 actgtgctgc ccgaggcggc cttgcaccgg cgcaggccgg tgacgacggg cgcggaacaa
     2401 caggtgcaca tcatgctggg aacagaaagc agtgtcgccg caagcacgct gcgattgctg
     2461 gcggccccca gccacttcct gaccaagtgc gccggcaaca gtgcctgcat ggcggaaccc
     2521 agcaacaaac cgagcagcag tgcctgccag atggaacggc cgtaagccca cgcgtaatcc
     2581 agggccgccc gcaacgacgg ggcgggtacc gtatccgccc ccgccgtcag catggattgg
     2641 ccaatcgagt gcatttgcgc ggcgacgaac gcgcggtcgt aatacggcga ccatttcaca
     2701 taaaacaatc caacaaccgc gagaagcaga aaaaccgcaa tcccccaggc tgggtgaaaa
     2761 gtgtgccgcg cacgcgaaaa attggtcacg aaaatctctg aaattggttg cacaacccga
     2821 cgtgtcggtg cggccgaaat gatacatcac cggtcttgac agtctcattt gttggtttat
     2881 agtagctata taaacaacta ctattaattg ttttatggtt tcgccagcca tttgaatagg
     2941 gtggtggcgc cggccaccgc ggtacaaccc tgatttttca caaggagttc catatgtcga
     3001 cgagctatga tgtgaaattg ctgcgcaagc aggaaatcgc tgaaggcacc ctggaattca
     3061 cactgcagaa gccccccggt ttcgagtacc gcgcgggcca gttcggtgat ctggtgctcc
     3121 cggcgtccac agggctggac gagagcaaca acaagcacgg cttctcgttc gtgagcgcgc
     3181 cttttgaaga aacgcttcgc atggccacac gcatgcgggc gacaagccgc tacaagcagg
     3241 cggccgccgg agtgccggag ggcacgatgg tgaagctgct cgccctgtgg ggcgatttca
     3301 cactgcagaa gaacgacaag atcccggccg tgttccttat cggcggcatc gggatcacgc
     3361 cggtccgaag catcatcgcg caggccacgc acgaaaagac ccgccacaag atcacgctca
     3421 tctactccaa ccgcacgccc gcgcaagcag cgtacgcgga cgaacttgcg cacttggccg
     3481 agatcaaccc caatttcagg tatgtcccgg tctacagcga cacggacggt ttcgtcacgg
     3541 ccgatacggt gcgtcggcat gtgccggacg tggcggcgcc gcgctactac ctgtccggcc
     3601 ccgaggggat ggtcaaagcc atgcgcaaga tcctggccga ggtgggggct gacgaggaca
     3661 acatcaagac tgaggaattc gagggttatt gattgagttt gccgccggac cgtctaggcc
     3721 acgttccccg cgatgccgtc caattcctca accgccgcgt cagacaacac aatttccacc
     3781 ttgattctgc cgtccatcgt tccgccattc ctggccgcgt atcccgatat tcgcctcgag
     3841 gcgatcgccg acgaaagaag tgtcagccat catggacaac ctgatggacg cgtcaaccgc
     3901 tatggactat gagcgacatg tactagactt tacagaccgt gccaaaagcg ctctgtcaaa
     3961 ggaggcgctg ttgtcaatct gtaagcacta tcaaagcaca agggggtttt tcgcaaagcg
     4021 tgagttcgtg gctgttttca ggcgtccgga gtccgttgcg gtcgtgtggc gtcaatggtt
     4081 caccaaacag ccgggagagt tcgtcgcgga aatggtgctg gtggaggtca atggcaagta
     4141 gcttgtcgac catgtcatgg ttttctagct cgcatacgaa atgccggaca ctctcatggc
     4201 aagcaatgtt ttggtgtggg tttggaacgc aaacaattca gtcaagcgga taatcaaata
     4261 accgagaccg agccacgcct cggacacggc gtcgctgtcg aatggagaca ataatgacct
     4321 caatctttga ccaagacctg ccccccaccg ctgccaattt cgccccgctg acgccgctgt
     4381 tgtatctgga gcgcgccgcc gaggtctacc cgcagcgcct ggcggtcgtg catggcctgg
     4441 gtgatacggc catccgccgc gactgggcgg acgtctatcg gcgttgtcgg cagctggcca
     4501 gtgccttggc gggccaaggc atcgggcgcg gggatacagt ggccgtcatg ctacccaaca
     4561 tcccggccat ggtcgaggca catttcggca taccgatgtg tggcgctgtt ctgaatacgc
     4621 tcaatacccg gctggacgcc gctacgctgg ccttcatgct ggatcacggc gaggcgcggg
     4681 ccgtgctcgt ggaccgggaa ttctccccgg tcatgcgcga ggctctagcc ctgcggcagc
     4741 gcaccgagcc cgttctactc atcgacgtgg atgaccccct atacgacggt cccggcgagg
     4801 cgatcggcac cctgggctac gaggccctgc tggaccaggg ggatccggac tacgcgtggc
     4861 tcatgcctgt atccgagtgg gatgccattt ctctgaacta taccagcggc accacgggca
     4921 acccgaaggg tgtcgtctat catcaccgag gcgcggctct caatgctgtg tcgaacatct
     4981 tggaatggga catgcccaag catgccattt atctgtggac gctgcccatg ttccattgca
     5041 atggttggtg cttcccctgg acggtggccg cgcgcgccgg ggtcaatgtc tgcctgcggc
     5101 gggtagacgt cgccgccatg gtcgatcgca tggtggagca tggtgtcacc cattattgtg
     5161 gggcgcccat cgtccacagc atgctggtca acgcgccgca ggctctgcgt gaccggattc
     5221 cgtccggcat tagtgccatg gtggccggcg ccgcgccgcc agcctcgatg atcgagggca
     5281 tggaatcgct gggcatcgat ctgacccacg tctacggcct gaccgaggtc tatgggccag
     5341 ccaccgtctg cgccaagcat gcggactggg atctcctgaa tatcggcgag cgggcccggc
     5401 tcaacgcccg tcagggtgtg cgctaccatc tggagcgatc ggccaccgtt ctgaatccag
     5461 ccaccatgca gccggtcccc gccgacggcc agaccatggg tgagatcatg ttccagggca
     5521 atatctgcat gaagggctat ctgaagaacc cgcaggccac ccaggaagcc ttcgcgggtg
     5581 gctggttcca cagtggcgat ctggctgtcg tcgacccaga cggctacatc aagatcaagg
     5641 atcgctccaa ggacatcatc atttccggcg gcgaaaacat ttcctcgatc gaggtcgagg
     5701 acgtgctgta ccgtcatccg gccgtgctgg ccgcggcggt ggtcgcgcgg cccgacgacc
     5761 gctggggcga gaccccctgc gccttcgtcg aactcaaggc cgacgcaagc gtcagtgccg
     5821 aggacatcat cgcgcactgc aaggcgcatc tggcgggttt caagatgcca cgccgggtcg
     5881 tctttggtga actgcccaag acatcgaccg gcaagatcca gaaattcgaa ctgcggcagc
     5941 gggcggcaaa agcgccatga cgcgccggaa ttcggggaaa ttgccgctcg caggggttga
     6001 tttgcgggct ttgggataca catgacacca tgatgaccac attcaagaaa gcggtttcgc
     6061 tcgcctcggc cctgttattc gcaggcctgt ttttccgcac agccgacgcc agtcaagtgc
     6121 atgagaccgc aaggaaattc atcatctatg agtacgtgcc ttactccact catatcgcgg
     6181 atggtgaagt tgtcacggag ccgtctttta agtcgtatct cgaatctctc ggcatccaca
     6241 ggattgatgt gatttatcgc aatcgattca tgaccgatgg gcgccccgac aagcagaaga
     6301 ttgaggacat tgccaagtcg gcgctcgcgg ctcccggcat cccggcctct ttcgacaccg
     6361 agttcggcca gcgcttcaag ccggagacgg tcatcccgcg ggtaagcgag atactccgca
     6421 ttttccatca ctacaacact gtcatccccg ccggggttta tgccacggcg ccacagaaca
     6481 ccttcgcatg gaagccgaac accccggccc gctttgacgc gctcaatccg cgttatgcaa
     6541 gcgtcgcgag gcaggtcgac ttccttagcc ccgtgctcta caactacaac ggcggcgaca
     6601 ctgcgcgttg gaggctaaac gccgactaca acatgcaagc cgcaaagaaa tatcacaccg
     6661 ggaaacccat aatcccctac atcaacatcg ctgtcgtctt gaaaaccagc aaacgggaca
     6721 agagagcgcc catcctggtt cgcatgctga ctgaagccca gatgcatgcg cggcttcagg
     6781 cgttgtacga ccttggtgcc agcggctgta tcgtctgggc cagtacccgg ttccgcacgg
     6841 ccgacggcaa aacgcccgta ttcgatcgca acagcggttg ggggaaagcg ctcgccgatt
     6901 ttgcgcaggc acatgggtaa gcgaaaccag cagcaaccca tttcaatcta tttttttgtg
     6961 aaccgcccaa accgaaaggg tgatggatcc acgcgtggcg ccacacctcg cacgaggtcc
     7021 gcgatcagtt ctcccgcggc cgggcccaag ccgaacccgt gccccgagaa ccccgtcgcg
     7081 atgaaaaggc cgtcgatctg tcccgcctgg ccgatgatcg gcacggcatc gggcgtcacg
     7141 tcaatcatcc ccgcccattc gtggctgatg cggcactgtg caaagccggg gaacgcgtgg
     7201 ctgagatttc gcacggcttc gtgattcagg cccggcgccg gatcgggatc cagaatccgg
     7261 gtttgctcaa aaggtgtggt gtcttgattg ctccaacgct tcgggacgcg caaatctttg
     7321 aatagctgtt ttcccgcgcg aaccctgagt tcccgccagc ctttggccag tgcgggcgca
     7381 aactctttga aaagacggaa gttgtcagtg gtgatctgcg cgatattggc gttgctgcgg
     7441 gcgatggtat atccgccgtc cgcccgtcgg cgaaacgcga agttgtcagc agcgacgggc
     7501 agtgcgggga tgcgaccgtc cagggtgtcg acgcgggcgg ccgttgccag cactttgagc
     7561 tgtggaatgg aaattccaag attcccggca aacaaccgcg accagctgcc gcctgccaac
     7621 acgacttgat cgcattggat cggaccttgt tctgtcacga caccgcacac gcggcccgct
     7681 tgtgtttcta tgctccgcac ggcacacgat tgcagaaggt gggcgccatg c
//
LOCUS       DASTNK010000137        16984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_666583, whole genome shotgun sequence.
ACCESSION   DASTNK010000137 DASTNK010000000
VERSION     DASTNK010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 16984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16984
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666583"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1148
                     /gene="paaA"
                     /locus_tag="VFR20_10175"
     CDS             159..1148
                     /gene="paaA"
                     /locus_tag="VFR20_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEU0230927.1"
                     /translation="MYAQLVDTGVARVRDTASLSGEEKAFQQRVDDGIRVEPKDWMPP
                     AYRKTLVRQISQHAHSEIVGMLPEGNWITRAPSLKRKAILLAKVQDEAGHGLYLYSAA
                     ETLGVSRDDLVDDLLTGKAKYSTIFNYPTLTWADIGMIGWLVDGSAIINQIPLCRCSY
                     GPYARAMVRVCKEESFHQRQGYDLLIQMCRHGTPAQKDMAQDALNRWWWPALMMFGPP
                     DAESVNSAQSMQWRIKLFSNDELRQKMVDQTVPQADYLGLTVPDPDLKWNEARGHYDF
                     GEIDWEEFHAVIRGNGPCNQQRLAARVRAHEDGEWVREAFSAHEHKRLRRKTA"
     gene            1238..1525
                     /gene="paaB"
                     /locus_tag="VFR20_10180"
     CDS             1238..1525
                     /gene="paaB"
                     /locus_tag="VFR20_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HEU0230928.1"
                     /translation="MQHSEWPLWEVFIRSQHGLAHKHVGSLHAPDAEMAINNARDVYT
                     RRNEGVSIWVVRSTDIVASSPDDKGPLFDPANSKTYRHPTFFPMPEEVKHM"
     gene            1534..2298
                     /gene="paaC"
                     /locus_tag="VFR20_10185"
     CDS             1534..2298
                     /gene="paaC"
                     /locus_tag="VFR20_10185"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEU0230929.1"
                     /translation="MDRNAFDYALRLGDTTLILSHRLSQWCGHGPALEEDLALTNVAL
                     DLLGQARMWLTLAGEAEGEGRDEDALAYLRDAPAYRNYLLVERENGHYGDTMARQFFF
                     DAWHYLLLERLAVSAHDGISAIAAKAVKEVAYHARRSADILVRLGDGTEDSHARMQQA
                     VDAAWPYVGELFVNDPTVEELAARQWAPRHGDLWPAWLEHVTSTLAEATLAQPDPAAA
                     HHPAHRGGYMGRHTEALGFLLAEMQHLQRAYPGATW"
     gene            2310..2819
                     /gene="paaD"
                     /locus_tag="VFR20_10190"
     CDS             2310..2819
                     /gene="paaD"
                     /locus_tag="VFR20_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004565836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEU0230930.1"
                     /translation="MDSSCAVAPEQVWRWLADVTDPEIPVLSIVDLGVVRDVVCGGGD
                     VEVTITPTYSGCPAMLAIEADIRRVLRAHGVASIRVLTRLAPAWTTDWMTARGRAALR
                     DYGIAPPQGGAATRCGLAALRAPRVAVVCPRCRSRSTELISEFASTSCKALYRCRACG
                     EPFDYFKPH"
     gene            2827..3909
                     /gene="paaE"
                     /locus_tag="VFR20_10195"
     CDS             2827..3909
                     /gene="paaE"
                     /locus_tag="VFR20_10195"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HEU0230931.1"
                     /translation="MDAFHSLNVTSVDRNTRDAVVVTFDVPVGLRDTFAFRPGQYLTL
                     RTSIDGQDLRRSYSICAAPDEGVLRVAIKRHDDGAFSNWANEHLVAGTRLDVMPPDGH
                     FTLAFSPEHRRGYAAFAVGSGITPILSLVKTALAVEPESRFALFYGNRASSAVLFREE
                     IEDLKNRYMNRLSVAYIMSREVQDIDLFNGRLDGDKVSQLLSHWMPPDAIDVAFVCGP
                     PDMTESVKGALLRAGLDATQIKFELFGAPRGPRALRTGHEARRAPGAGRCEVTVTHDG
                     LTRTLMVEKNRDSILDSALSQGIELPYSCKGGVCSTCRCKVIEGEVDMDANFALEDYE
                     VARGFILCCQSFPVTDRLVLDFDQET"
     gene            3955..5619
                     /gene="paaN"
                     /locus_tag="VFR20_10200"
     CDS             3955..5619
                     /gene="paaN"
                     /locus_tag="VFR20_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaN"
                     /protein_id="HEU0230932.1"
                     /translation="MSTDLFEKHKALLDRAVAATDERGYWSPFSESPSPRAYGETAAE
                     DGRHAFEAHRGADFDLGQGGATSWVGGEKSPFGFDLGVRYPHVPAQVMVERADEALES
                     WRAAGPQAWVGVALEILTRLNRHSFEIAQAVQHTTGQGFMMAFQAGGPHAQDRGLEAV
                     AYAWREMSRLPGAALWEKPQGKHDPLRMEKRFTVVPRGVALVIACSTFPTWNSYPGLF
                     ASLATGNTVIVKPHPTAILPLAITVRIARDVLREAGFDPDVVSLAAHEAGDDTAQALA
                     LSPAVRLIDFTGSSANGTWLEQNARQALVFTEKAGVNPVVIDSADDFAGVARNLAFSF
                     ALYSGQMCTAPQNVYIPRDGVDTASGHLSFEQAAGALAEAIGKLTADAARAVEITGAI
                     QNAATLARIDEARATGLPVLCDSRALVHPQFPDAVVRTPLLLQAQADSPVIQKEWFGP
                     IAFVVATDSTQASVDLARCSVQTHGALSFSGYTTRQVVSDALCAAAEHAGVSLSLNLT
                     GGVFVNQTAAFSDFHGTGANPAANAALSDSAYVANRFRVVQTRWHV"
     gene            5667..6452
                     /gene="paaG"
                     /locus_tag="VFR20_10205"
     CDS             5667..6452
                     /gene="paaG"
                     /locus_tag="VFR20_10205"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HEU0230933.1"
                     /translation="MNFENIEFSLKEGVARIALNRPTKLNSFTVAMHGELSRALTEVE
                     QGGARVLLLTGNGRGFCAGQDLSERQMSGGQAVDLGHTIETYYAPLVRRLRALPMPVV
                     VGVNGVAAGAGANLALAGDIVIACKSASFIQPFCRLGLLPDTGGTYALPRLVGQARAM
                     GLALLGDKLDAEQAAQWGLIWQCVPDDQFQDRLDSLLAHFASAPTKGLAYAKRALNAS
                     LDNTLDQQLDLERDLMRELGASHDYAEGVGAFLEKRAPRFKGA"
     gene            6533..6934
                     /gene="paaI"
                     /locus_tag="VFR20_10210"
     CDS             6533..6934
                     /gene="paaI"
                     /locus_tag="VFR20_10210"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HEU0230934.1"
                     /translation="MYRNDAASQGLGMRLVRVAPGRARMEMTVRPDMLNGHQTCHGGF
                     IFSLADSTFAFACNSRNQATVASGCTIDFLAPGYAGDVLTADCEEQSLSGRTGVYDVV
                     VTNQEGRRVALFRGRSYRIKGQVIPQAGDES"
     gene            6931..8244
                     /gene="paaK"
                     /locus_tag="VFR20_10215"
     CDS             6931..8244
                     /gene="paaK"
                     /locus_tag="VFR20_10215"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HEU0230935.1"
                     /translation="MTTTSGHPGRDAVEHASRDELAALQLQRLKWSLSHAYEHVPHYK
                     AAFEAADVHPDDLRQLSDISRFPFTIKQDLRNNYPFGMFAVPREQVVRIHASSGTTGQ
                     PTVVGYTANDISIWADLVARSIWAAGGRPGDIVHVAYGYGLFTGGLGAHYGAERLGCS
                     VVPMSGGQTEKQVRLIHDFGPRVIMVTPSYFCNILEEMARQGLDPHASSLKVGIFGAE
                     PWTDRMRADIEQRSGIDAVDIYGLSEVMGPGVAQECVESKDGPVVWEDHFYPEIIDPQ
                     TGEVLPDGELGELVFTSLSREAMPVIRYRTRDLTRLLPPTSRGMRRIDRITGRSDDML
                     IVRGVNLFPTQVEELILKMPELAAQYQLVLTREGHLDALEIVAEVRGEFDAMGADARA
                     GLVGRLVQAVKNNIGVTASVTLKGSGQIERTITGKARRVLDKRPK"
     gene            8413..8853
                     /gene="acpS"
                     /locus_tag="VFR20_10220"
     CDS             8413..8853
                     /gene="acpS"
                     /locus_tag="VFR20_10220"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU0230936.1"
                     /translation="MIATAGTPGGIAGIGVDLTRIARIERVYARHGARFVQRILGPEE
                     IPVFERRHRRDPARGMRFLASRFAAKEAFSKAIGLGMHDPMAWSRMQTLNAPGGKPVV
                     SLREPLAGWYSARFGAAHVSITDESDVVAAFVVVEAHGPAPSSH"
     gene            8876..9937
                     /gene="nagZ"
                     /locus_tag="VFR20_10225"
     CDS             8876..9937
                     /gene="nagZ"
                     /locus_tag="VFR20_10225"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU0230937.1"
                     /translation="MTARQHSRTPCPPGPVMVDVAATSLTPHEKKRLRHPLVGGVILF
                     ARNFENRGQLEALCRDIHQARSEPLLIAVDHEGGRVQRFRTDGFTAIPAMGLLGEFWM
                     REPLGAVRLATEAGYVLGAELRACGVDLSFTPVLDLDHGRSRVIGDRAFHADARVVAM
                     LARGLIQGLSLAGMAACGKHFPGHGYAEADSHHALPVDDRSLADILKDDAAPYDWLGD
                     MVLPSVMPAHVVYPRVDDQPAGFSRVWIQQVLRGDLAYDGVVFSDDLTMQGATIAGDI
                     LARARAALGAGCDMVLVCNRPDLADELLARLDVAPSPDSVRRVRRLMPAAAAPDWNTL
                     QADSRYQHARALQSQVVPG"
     gene            9900..11723
                     /gene="uvrC"
                     /locus_tag="VFR20_10230"
     CDS             9900..11723
                     /gene="uvrC"
                     /locus_tag="VFR20_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU0230938.1"
                     /translation="MPEPFNLKSFLADLPHLPGVYRHLDAEGEVLYVGKARDLKKRVS
                     SYFQKTQPSPRIAHMVARVARLEVTVTRSEAEALLLENNLIKSLRPRYNILFRDDKSY
                     PYLRITGHKAPRIAYYRGATSGKGRYFGPYPNAWAVRETIQILQRVFQLRTCEDSVFA
                     NRSRPCLLHQIGRCSAPCVGVISPEDYQRDVERAIRFLDGQALQVMDDIEARMRQASD
                     NLQFEQAATLRDQMKALARVLQQQSMEQIGDDDVDIIAVAGAGGRVCVNLAMVRGGRH
                     LGDKPFFPTQTQDDSAGDVLSAFVGQHYLDGALPAVLVCSHPLSDPELVDLLAAQKGV
                     KARVITRPQGLRRTWLEQAVKNAEMALARLLTESGARAARTEALAGTLGLDTDEAALD
                     ALHAECFDISHTAGEATQASCVVYKHHAMQPSLYRRYNIAGITPGDDYAAMRQVLTRR
                     FSRVAEGQAELPSLVLIDGGKGQVEMARQVFVELGLDTHTIVGVAKGEGRKVGLETLV
                     FADGRPSVALGVESAALMLVAQIRDEAHRFAITGMRAKRAKARNVSRLEEIPGVGARR
                     RQRLLARFGGFSGVVAASIDDLCSVEGISGELAERIYQALR"
     gene            11981..12559
                     /gene="pgsA"
                     /locus_tag="VFR20_10235"
     CDS             11981..12559
                     /gene="pgsA"
                     /locus_tag="VFR20_10235"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU0230939.1"
                     /translation="MPINLPIILTWLRIAMIPVLVGLFYIPSGWLSFPVRDLIAALAF
                     IVAAVTDWLDGWLARRWNQTSSFGAFLDPVADKLMVSAALLILLHLGRVDILIALVIV
                     GREIAISALREWMAKIGASGSVAVHWLGKFKTAAQMVAIPCLLYWQPVQGVSARVVGQ
                     VLIVIAAILTVWSMCYYLRRAWPEIRDRSQAF"
     gene            complement(12566..13390)
                     /gene="queF"
                     /locus_tag="VFR20_10240"
     CDS             complement(12566..13390)
                     /gene="queF"
                     /locus_tag="VFR20_10240"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523221.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEU0230940.1"
                     /translation="MNESGLANAPLGHDIRYPDHYDPALLFPIAREANRAALAPLPPW
                     TGADIWTAYELSWLNPRGKPMVGTARFVFPESSPCLIESKSLKLYLNSFTETRFDGTE
                     AVRHLVEQDLSHAAGAPVRVWLDRLNANRSEVPQPLSGTNIDDLDVEIQHYQPVPGLL
                     RCLPDAAVVDETLVSDLLKSNCPVTGQPDWGSVQVRYRGRRIDPASLLQYIVSLRRHK
                     EFHEHCVEQMYCDIWRACQPEWLLVYARYTRRGGLDINPWRASSAIDVSLSRTPRQ"
     gene            13708..15036
                     /gene="aceA"
                     /locus_tag="VFR20_10245"
     CDS             13708..15036
                     /gene="aceA"
                     /locus_tag="VFR20_10245"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEU0230941.1"
                     /translation="MTTRETEIRRLQENWAEDPRWQGVKRGYGAEEVVRLRGSVPVEH
                     TLARRGAEKLWGLLGSEPFVNSLGALTGNQAMQQVKAGLKAIYLSGWQVAGDANGAGE
                     MYPDQSLYPANSVPQVVRRINNALTRCDQIQWMEGANPGDPGYVDYFAPIVADAEAGF
                     GGVLNAFELMKSMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTREAVAKLVSARLAA
                     DVLGVPTVLLARTDADAANLLTSDVDENDRPFITGERTAEGFFQTRAGIDQAISRGLA
                     YAPYADLLWSETSTPNLEYARKFAEAIHRRFPGKLLAYNCSPSFNWRKNLDDATIAKF
                     QRELGAMGYKFQFITLAGFHALNYGMFELAHGYARRQMSAFVELQQKEFAAADLGFTA
                     VKHQREVGTGYFDAVTQTIEGGRSSTTALSGSTEEAQFEDDGGQTGLRVA"
     gene            complement(15094..15423)
                     /locus_tag="VFR20_10250"
     CDS             complement(15094..15423)
                     /locus_tag="VFR20_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEU0230942.1"
                     /translation="MSKRYIPDTGDIIHVHFEARGRGASSSYKPAMVLSPQAYNKRTG
                     LMVCCPITPSIKGYPFEVPIEGGAVLSDQVKSLGWADSKISFHGRIGPADLAQVRAKL
                     HALIFKD"
     gene            complement(15435..15665)
                     /locus_tag="VFR20_10255"
     CDS             complement(15435..15665)
                     /locus_tag="VFR20_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEU0230943.1"
                     /translation="MRGVIKKWGNSAAVRLPSSMIKTLKLGLEDMIDIRVERGTILIE
                     PVRTGEYVLSQLLSGVKARNLHPEVDFGAAGD"
     gene            16034..>16984
                     /locus_tag="VFR20_10260"
     CDS             16034..>16984
                     /locus_tag="VFR20_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEU0230944.1"
                     /translation="MRFDKLTTRFQQAIADAQSLAARHDNPYIEPVHVLAALLSDADS
                     GAGSLLARAGVGVNRLVSSVNTVIAALPQVAGSEENLQVSRDLNTLLTRTDKEAAGRG
                     DTYIASELFLLVLADDKGEAGRLLREAGLRKNALETAIDAVRGGAPVADSEGEANREA
                     LAKYTLDLTEKARQGKLDPVIGRDDEIRRTIQILQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNDEVPETLKGKRVLALDLAALLAGAKFRGEFEERLKAVLKELAQDDGQTIVF
                     IDELHTMVGAGKAEGAMDAGNMLKPALSRGELHCVGATTLD"
BASE COUNT         3036 a         5195 c         5562 g         3191 t
ORIGIN      
        1 aggatgccgt tccagaaggc tggaaaccag gggttgaagc aggtcggcgc gtgtgtccat
       61 gcgtaccctg acgtgatact gttttttgat tgacttcaaa aattctgtat taaataatat
      121 agaaaacgct tccgctacac aatggccagg agacgcctat gtatgctcag ctcgttgata
      181 ccggggtggc gcgcgtgcgc gacacggcct cgctgtccgg cgaggaaaag gcttttcagc
      241 agcgcgtgga cgacggcata cgcgtcgaac ccaaggactg gatgccgccg gcctaccgca
      301 agacgctggt gcgccagata tcgcagcatg cccattccga gatcgtcggt atgctgccgg
      361 aaggcaactg gatcacgcgg gcgccgtctc tcaagcgcaa ggccattctt ctggccaagg
      421 tgcaagacga agccggccat ggtctgtatc tgtacagcgc cgccgaaacc ctgggggttt
      481 cgcgcgacga tctggtcgat gatctgttga ccggcaaggc caagtattcg accattttca
      541 attaccccac gctcacctgg gccgatatcg gcatgatcgg ctggctggtc gacggctcgg
      601 ccatcatcaa ccagatcccg ttgtgccggt gctcgtacgg cccgtatgcg cgcgccatgg
      661 tgcgcgtctg caaggaagag tcgttccatc agcgacaggg gtacgacctg ctcatccaga
      721 tgtgccggca tggcactccg gcccagaaag acatggcgca ggatgcgctg aaccgctggt
      781 ggtggccggc cctcatgatg ttcggcccgc ctgatgccga atcggtcaat agcgcccagt
      841 ccatgcaatg gcgcatcaaa ctgttctcga acgacgaact gcgccagaaa atggtcgacc
      901 agaccgttcc gcaggccgac taccttggcc tcaccgttcc cgatcctgac ctgaaatgga
      961 acgaggcgcg cgggcactac gatttcggtg agatcgactg ggaagagttc cacgcagtga
     1021 ttcgcggcaa cggtccgtgc aaccagcaac ggctggccgc gcgcgttcgc gcccacgaag
     1081 acggcgagtg ggtgcgcgag gccttttccg cgcatgaaca caagcggctg cgacgcaaga
     1141 cggcctgagg cgaggggtat cccaggatcg atgccccgtt cgcgatgaac cacgggtgat
     1201 gaaccgcggg cgatgaagca cacataaagg aacgatgatg caacacagtg aatggccctt
     1261 gtgggaagtg ttcatccgca gccagcatgg cctggcccac aagcacgtgg gaagcctgca
     1321 cgcgcccgac gcggaaatgg ccatcaacaa cgcgcgggat gtctatacac gccgcaacga
     1381 gggagtgagc atctgggtcg tgcgctcgac cgacatcgtg gccagcagcc ccgacgacaa
     1441 agggccgttg ttcgatccag ccaacagcaa gacataccgg catcccacgt tcttcccgat
     1501 gcccgaagaa gtcaagcaca tgtgagcggc gccatggatc gtaatgcctt tgattatgcc
     1561 ttgcgcctgg gtgacaccac gctgatcctg tcgcatcgcc tgtcgcaatg gtgcgggcac
     1621 gggccggccc tggaagaaga cctggcgttg acgaacgtcg cgcttgacct gctggggcag
     1681 gcgcgcatgt ggctcaccct ggccggcgag gccgaaggcg aggggcgcga cgaagatgcc
     1741 ctggcctacc tcagggacgc gccggcttac cgcaactatc tgctggtcga gcgcgaaaac
     1801 ggccattacg gcgacaccat ggcgcgccag tttttcttcg acgcttggca ttatctgttg
     1861 ctggagcgcc tggccgtgtc cgcgcatgac ggcatctcgg ccattgccgc caaggcggtc
     1921 aaggaagtgg cctaccatgc ccggcgatcc gccgatatcc tggtgcggct cggcgacggc
     1981 acggaagaca gccatgcccg catgcagcag gcagtcgacg ccgcctggcc ttacgtgggt
     2041 gaattgttcg tcaacgaccc gacagtcgag gagctcgcgg cgcggcaatg ggcgccccgg
     2101 catggtgatc tctggccagc atggctggaa cacgttacct cgaccctggc cgaggcgacg
     2161 ctggcccaac ccgaccccgc agcggcgcac catccagcgc atcgtggcgg ctacatgggg
     2221 cgccacaccg aggcgctggg ctttctactg gccgaaatgc agcacctgca acgcgcttat
     2281 ccgggggcga catggtagtt gaggccgccc tggattcgtc gtgcgccgtg gcaccagagc
     2341 aagtctggcg ctggctggcg gacgtgacgg atcctgaaat ccccgtcttg tccatcgtcg
     2401 acctgggagt ggtgcgcgac gtcgtctgcg gtggtggcga tgtcgaggtc accatcacac
     2461 ccacctattc gggctgcccg gccatgttgg ccatcgaggc cgacatccgc agggttttgc
     2521 gggcgcacgg cgtggcgtcc atacgagtgc tcacccgcct ggcgccggcc tggacgaccg
     2581 actggatgac cgcccggggg cgtgccgcgc tacgcgacta cggcatcgcg ccaccgcaag
     2641 gcggtgcggc cacgagatgc ggactggccg cgctgcgcgc cccgagggtg gccgtcgtgt
     2701 gcccgcgttg ccggtcgcgc tcgaccgagc tgatcagcga attcgcctcg acctcctgca
     2761 aggccctgta ccggtgccga gcctgcggtg agccgtttga ctacttcaag ccacactgag
     2821 cccgccatgg atgcctttca ctccttgaac gtgacgtccg tggaccggaa cacgcgcgac
     2881 gccgtggtgg tcaccttcga cgtgcccgtt ggtctacgcg acaccttcgc gttccgcccc
     2941 ggccagtacc tgacgttgcg caccagcatc gacggccagg acttgcgccg ttcctattcc
     3001 atttgcgccg cgcccgacga gggggtgctg cgcgtggcaa tcaagcgcca tgacgatggc
     3061 gcgttctcca attgggccaa cgagcatctg gtggccggaa cacggctcga cgtcatgccg
     3121 cccgacggcc atttcacgtt ggcgttctcg ccggaacacc ggcgtggtta cgcggccttc
     3181 gcggtcggca gcggcatcac gcccatcctg tcgctggtca agacggcgct cgcggtcgaa
     3241 ccagaaagcc gcttcgcact gttctatggc aaccgagcct cttcggcggt cctgtttcgg
     3301 gaagaaatcg aggatctgaa gaaccgctac atgaaccgtc tgtcggtggc ctacatcatg
     3361 agccgcgagg ttcaggacat cgatcttttc aacggccggc ttgatggcga caaggtatcg
     3421 cagctgctgt cgcactggat gcctcccgac gcgatagacg tggcgttcgt atgtggtccg
     3481 cccgacatga ccgagtcggt caagggggcg ttgctgcggg cggggctgga cgccacgcag
     3541 atcaagttcg aattgttcgg cgcgccgcgc ggcccgcgtg ccctgcgcac cggccacgag
     3601 gcccggcgtg cgccgggggc ggggcgctgc gaggtcaccg tcacccatga cggcctgaca
     3661 cgcaccctga tggtcgagaa gaaccgcgac agcatcctgg attcggccct gtcacaaggt
     3721 attgaattgc cttattcgtg caagggcggc gtttgttcca cctgtcgctg caaggtcatt
     3781 gagggcgagg tcgacatgga cgccaacttc gcgctggaag attatgaagt ggcgcgtggg
     3841 ttcatcctgt gctgccagag tttcccggtc accgaccgtc tggtgctgga tttcgaccag
     3901 gaaacctgag gtccggaaaa atcatcaagg cgaatcatca caaggagttc cacattgtcc
     3961 acggatctat ttgaaaaaca caaagccctg ctcgatcggg cggtggcggc gacggacgag
     4021 cgcggctact ggagtccgtt ttcagagtca cccagcccgc gtgcctatgg cgaaaccgcc
     4081 gccgaggatg gacgccatgc attcgaggcg catcgcggtg ctgatttcga tctggggcag
     4141 ggcggcgcga cgtcatgggt cggtggcgag aagtcgcctt ttggattcga tctgggtgtg
     4201 cgttatcccc acgtgccggc ccaggtaatg gtcgaacgcg cggacgaggc gcttgaatcc
     4261 tggcgcgcgg cgggtccgca ggcgtgggtg ggggtggctc tggagatcct cacgcgtctc
     4321 aaccgccaca gctttgaaat cgcccaggcc gtccagcaca cgacggggca ggggttcatg
     4381 atggcgttcc aggcgggcgg cccgcatgcc caggaccgtg ggctggaggc cgtggcatac
     4441 gcctggcgcg agatgtcccg cctgcccggc gccgcgcttt gggaaaagcc gcagggcaag
     4501 catgatccgc tgcgcatgga aaagagattc actgtggtgc ccaggggtgt ggcgctggtg
     4561 atcgcctgct ccaccttccc cacctggaac agctaccccg gcttgttcgc cagtctggcc
     4621 acgggcaaca ccgtgatcgt caagccgcat cccaccgcga tcctgccgct cgccatcacc
     4681 gtgcgcatcg cgcgcgacgt gctgcgtgaa gccggattcg atccggacgt cgtgtcgctg
     4741 gcggcgcacg aagccggtga tgacaccgcg caggcattgg cgctgtcacc cgccgtgcga
     4801 ctcatcgact tcacgggcag cagcgcgaac ggcacgtggc tcgaacagaa cgcgcgccag
     4861 gcgctggtct tcaccgagaa agccggcgtc aatccggtgg tgatcgattc ggccgacgat
     4921 ttcgccggtg tggcacgcaa tctggcattc tctttcgccc tgtattccgg tcagatgtgc
     4981 acagcgccgc aaaatgtcta tattccccgc gatggcgtcg acacggcgtc cggccacctg
     5041 agcttcgagc aggccgcggg cgcgctggcc gaggcgatcg gcaaactgac cgccgatgcc
     5101 gcgcgtgccg tcgagatcac cggcgccatc cagaacgcgg ccacactggc gcgcatcgat
     5161 gaggcgcggg cgaccgggtt gccggtgctc tgcgacagcc gcgcattggt ccatccgcaa
     5221 tttcccgatg cggtggtgcg cacgccgctg ctgcttcagg cccaggccga cagcccggtg
     5281 attcagaagg aatggttcgg tccgatcgcc ttcgtcgtgg ccaccgattc cacgcaggcc
     5341 agcgtggatc tcgcccggtg ctcggtgcaa acgcatgggg cgctgtcgtt ctcaggctac
     5401 accacccggc aggtggtgtc ggacgcgctt tgtgccgctg ccgagcatgc gggggtgtcg
     5461 ctgtcgctga acctgactgg cggcgtgttc gtgaaccaga ctgccgcatt cagtgatttc
     5521 cacggaacgg gcgccaaccc ggcggccaat gccgccttgt ccgattccgc ctatgtcgcc
     5581 aaccgcttcc gtgtcgtgca gacgcggtgg catgtctgat tctcgaggaa agtggccgtg
     5641 aacatttttg ccatggaggc gacaacatga attttgagaa catcgagttc agcctcaagg
     5701 aaggcgtggc tcgcatcgcg ctgaatcgcc ccaccaagct caacagtttc accgtcgcca
     5761 tgcacggcga actgtcccgc gccttgacgg aggtcgagca gggcggggcg cgtgtgctgc
     5821 tgctcaccgg caatggccgg ggtttctgcg ccggacagga tctgtccgaa cgccagatga
     5881 gcggcggtca ggccgttgat ctcggtcata ctattgaaac ctactatgcc ccgctagtgc
     5941 ggcgcctgcg tgcgctgcca atgccggtgg tggtgggagt caacggggtc gccgccggcg
     6001 cgggggcgaa tctggccctt gcgggcgaca tcgtgatcgc ctgcaagtcg gcgagcttca
     6061 tccagccatt ctgccgtctg ggcctgttgc ccgacaccgg cggcacgtat gccctgccgc
     6121 gtctggtggg gcaggcgcgc gcgatgggcc tggccctgct gggcgacaag ctcgacgcgg
     6181 agcaagcggc acagtggggc ctgatctggc agtgcgtgcc ggatgatcag ttccaggaca
     6241 ggctggattc cctgcttgcg catttcgcca gcgcacccac caaggggctg gcctatgcga
     6301 agcgggcctt gaacgccagc ctggacaaca cactcgacca acagctcgat ctcgaacgcg
     6361 acctcatgcg cgaactgggc gcgagtcacg actacgcgga aggggtcggc gcctttcttg
     6421 aaaaacgcgc tccccggttc aagggggcat gatgcataca tccacttcag cgcggccgga
     6481 acccctcgtg ccggacgacg aaaatcttct ggctgaacaa acaggccagg tcatgtatcg
     6541 caacgacgcc gcgtcgcagg gcctgggcat gcgcctggtg cgcgtcgccc cgggtcgggc
     6601 gcgaatggaa atgacggttc ggccggatat gctcaacggg caccagacct gtcatggcgg
     6661 cttcattttc tcgttggccg acagcacttt cgctttcgca tgcaattcgc gcaatcaggc
     6721 gacggtcgcc tcgggttgca cgatcgactt cctggcgccc ggctacgccg gtgatgtgct
     6781 gacggcggac tgcgaagagc aatcgttgag tggccgcacc ggcgtgtacg acgtggtggt
     6841 gaccaaccag gaggggcgca gggtcgcgtt gttccggggg cgttcgtatc gcatcaaagg
     6901 gcaggtcata ccacaagcag gagacgaatc atgacaacga catcgggaca tccgggccgc
     6961 gacgcggtgg agcacgccag ccgggatgaa ttggccgcgc tgcagttgca gcgcctgaag
     7021 tggtcgctta gccacgccta tgagcacgta ccgcattaca aggcggcgtt cgaggcggcg
     7081 gacgtgcatc cggatgatct caggcaactc tcggatattt cccggtttcc cttcaccatc
     7141 aaacaggacc tgcgcaacaa ttatccgttc ggcatgttcg ccgtgccgcg cgagcaggtg
     7201 gtgcgcatcc acgcgtccag cggcaccacc ggtcagccga cggtggtcgg ctacacggcc
     7261 aacgacattt ccatctgggc cgatctcgtc gccagatcga tctgggcagc gggcggtcgg
     7321 cccggcgaca tcgtgcatgt cgcctacggc tatggcctgt tcaccggcgg gctgggcgcg
     7381 cattacggcg ccgaaaggct ggggtgctcg gtggtgccga tgtccggcgg ccagacagaa
     7441 aagcaggtgc gcctgataca cgacttcggc ccgcgcgtga tcatggtcac cccgtcatac
     7501 ttctgcaata ttcttgaaga gatggcgcgg caggggttgg atccgcacgc cagctcactg
     7561 aaggttggca tctttggcgc cgaaccctgg accgaccgca tgcgtgccga tatcgaacag
     7621 cgcagcggca tcgacgcggt ggatatctat gggctgtcgg aagtcatggg gccgggcgtg
     7681 gcgcaggagt gtgtggagtc caaggatggc ccggtggtgt gggaggatca tttctatccg
     7741 gagatcatcg atccgcaaac gggcgaggtg ctgcctgatg gcgagctcgg cgagctggtg
     7801 ttcacgtcgc tttcgcgcga agccatgccg gtgatccgct atcgcacacg cgacctgacc
     7861 cggctgttgc cgcccacctc gcgcggcatg cggcgcatcg accgcatcac cggccgcagc
     7921 gacgacatgc tgatcgtgcg cggcgtgaat ctgttcccca cccaggtcga ggaactgatc
     7981 ctcaagatgc ccgaactggc ggcgcagtac caactcgtgc tgacacgcga gggccatctc
     8041 gacgcgctcg agatcgtggc ggaagtccgt ggtgaattcg acgccatggg cgccgatgcg
     8101 cgcgccggac tcgttggcag actggtgcag gcggtcaaga acaacatcgg cgtcaccgct
     8161 tcggtgacgc tgaaagggtc cgggcagatc gagcgcacga tcaccggcaa ggcgcgccgc
     8221 gtgttggaca agcgcccgaa gtagttcgga tcgtccatca ccgtggcgcg tcgcctcgtt
     8281 cgaggccctg ttcgatcggg gcaggccacg tcgccagcaa ggcaggccgc gctcacggta
     8341 tgatgcggtt ttatccggtt gccggcgcca cgaatccgag gctgtgccgg ctcggtcgaa
     8401 ccaggcgggc tcatgattgc aacggcggga actccaggcg gcatcgcggg aatcggcgtc
     8461 gatctgacac gtatcgcacg tatcgaacgc gtttacgcgc gccatggtgc caggttcgtc
     8521 cagcgtatcc tgggccccga ggaaatcccg gtgttcgagc ggcgccaccg gcgcgatccc
     8581 gcgcgtggca tgcgtttcct ggcatcgcgc ttcgccgcca aggaagcgtt ttccaaggcc
     8641 atcggtctgg gcatgcacga ccccatggcc tggtcccgca tgcaaaccct gaacgctccc
     8701 ggtggcaagc cggtggtcag cctgcgtgaa ccgctcgcgg gctggtattc ggcccgcttc
     8761 ggggcggcgc atgtttcgat cacggatgaa agcgacgtcg tggcggcctt cgtggtcgtc
     8821 gaggcacacg ggccggcgcc ttcttcccat tgattttcat ccagactgaa tcagcatgac
     8881 agccagacaa cacagtagga caccgtgccc gccgggcccg gtgatggtgg atgtggccgc
     8941 cacatccttg acgccgcacg aaaaaaagcg gttgaggcac cccctggtgg gcggcgtgat
     9001 cctgttcgcg cgcaatttcg agaatcgcgg acagctcgaa gccttgtgcc gtgacatcca
     9061 tcaggctcgg tctgaacccc tgttgatcgc cgtggaccac gagggcgggc gggtccagcg
     9121 tttccgcacg gatggcttca ctgccattcc cgcaatgggc cttcttggcg agttctggat
     9181 gcgcgagccg ttgggggcgg tgcggctggc gaccgaggcg ggttatgtgc tgggagcgga
     9241 gttgcgggcc tgtggcgtgg acctcagttt cacccccgtg ctcgatctcg accatgggcg
     9301 cagccgcgtc atcggcgatc gggcgttcca tgccgatgcg cgggtcgtgg ccatgctggc
     9361 gcggggcttg atacaggggc tgagcctggc gggcatggcg gcatgcggca agcattttcc
     9421 cggccacggc tacgcggaag cggattccca tcatgcgctt cccgtggatg atcgttccct
     9481 ggccgatatt ctgaaggacg acgcggcgcc ttacgattgg ctgggcgata tggtgttgcc
     9541 gtcggtcatg ccggcgcacg tcgtctatcc ccgggtcgac gaccagcctg cggggttttc
     9601 gcgtgtgtgg atccagcaag tgttgcgtgg cgatctggcc tacgacgggg tggtgttctc
     9661 ggacgatctc accatgcagg gcgctaccat cgccggcgat atcctggcgc gggcgcgtgc
     9721 cgccctgggc gccggatgcg acatggtcct ggtgtgcaat cgccccgatc tcgccgacga
     9781 gctattggcg cgccttgacg tcgccccgtc gcccgattcg gtgcgacgtg tgcgccgcct
     9841 gatgcctgcc gcggcggcgc cggactggaa tactctgcag gcggattcac gttatcaaca
     9901 tgcccgagcc cttcaatctc aagtcgttcc tggctgacct gccgcacctg cccggtgtct
     9961 atcggcatct cgacgccgaa ggtgaggtgc tgtacgtcgg caaggcgcgc gatctcaaaa
    10021 aacgggtttc gtcctacttc cagaagaccc agcccagccc gcgcatcgcc cacatggtgg
    10081 cgcgcgtcgc gcgcctcgag gtcaccgtga cccggtccga ggccgaggcg ctgctgctgg
    10141 aaaacaacct gatcaagtcg ctcagaccgc gctacaacat tctgttccgc gacgacaagt
    10201 cgtatccgta tctgcgcatc actggccaca aggcgccacg catcgcgtac tatcgcggcg
    10261 ccaccagtgg caaggggcgg tacttcgggc cctacccgaa cgcctgggcc gtgcgcgaga
    10321 ccattcagat tctgcagcgc gtgttccagt tgcgcacatg cgaggacagt gtcttcgcca
    10381 accgatcccg gccgtgcctg ctgcatcaga tcggacggtg ctcggcgccg tgcgtggggg
    10441 tgatttcccc cgaggactac caacgcgatg tcgaacgcgc catccgtttc ctggatggcc
    10501 aggcgctgca ggtcatggac gacatcgagg cccgaatgcg ccaggcctcc gacaatctac
    10561 agttcgagca ggccgccacc ttgcgcgacc agatgaaggc gctggcccgt gtgctgcagc
    10621 aacagtccat ggagcagatc ggcgacgatg atgtcgacat catcgcggtc gccggcgccg
    10681 gtggccgggt gtgcgtgaat ctggccatgg tgcggggcgg gcgccacctt ggtgacaaac
    10741 cgtttttccc cacgcagacg caagacgaca gcgcggggga tgtcttgtcg gcgtttgtgg
    10801 gccagcatta tcttgacggc gcgttgcctg ccgtgctggt ctgttcgcat ccgttgtcgg
    10861 accccgagct ggtcgatctg ctggccgcgc agaaaggggt gaaggcgcgt gtcatcactc
    10921 gtccgcaagg actgcgccga acctggctcg agcaggcggt caagaatgcc gagatggcgc
    10981 tggccaggct gctcacggaa tcgggggctc gcgccgcccg taccgaggcg ctggccggca
    11041 cgctggggct cgacacggac gaggcggcgc tcgatgccct gcatgcggaa tgtttcgaca
    11101 tcagccatac ggctggagag gcaacccagg catcctgcgt ggtctacaaa catcacgcca
    11161 tgcagccctc gctgtatcgg cgctacaaca tcgccggcat cacgccgggt gatgattacg
    11221 ccgcgatgcg ccaggtgctc acccggcgtt tttcgcgcgt ggcggaaggg caggccgagc
    11281 tgccatccct ggtgctgatc gacggcggca agggccaggt ggaaatggca cgccaggtgt
    11341 tcgtcgagct ggggctggat acgcacacca tagtcggcgt ggcgaaggga gaggggcgca
    11401 aggtcgggct cgagacgctg gtgttcgccg atggccgtcc gtccgtggcg ctgggcgtgg
    11461 aatccgcggc cctgatgctg gtggcccaga ttcgggacga ggcgcaccgt tttgccatca
    11521 ccggcatgcg agccaagcgc gccaaggcgc gcaacgtgtc gcggctcgag gaaattcctg
    11581 gtgtcggtgc acggcgtcgt cagcggctgt tggcccggtt tggcgggttt tccggcgtgg
    11641 tggcggccag catcgacgat ttgtgctcgg tcgaaggcat ttccggcgag ctcgccgaac
    11701 gtatctatca ggcgttgcgc tagtgtcctg tttgattggt tcgtacactt gtattcggat
    11761 gcatgggctt gccaggcaag gcgcaaaccg cagggatagc agtggctatc gcgaggattt
    11821 gcaacgcccc atggcgagtc caggcgccga agataagcag ccgaattagt caaacaggac
    11881 actaggggcc ttctgaacaa gccccctgat tgatgtgttt ttttggcgaa tcccttgcct
    11941 cggacactgg ctccgccccg gcataggtta gtattgccgt atgccgatca atctgcccat
    12001 tattctcacc tggctgcgca tagccatgat cccggttctg gtgggattgt tctacattcc
    12061 gtcgggctgg ctgtcgtttc ccgtgcgcga tctcatcgcc gccttggcgt tcatcgtggc
    12121 ggccgtcacc gattggctgg acggctggct ggcccgtcgc tggaaccaga catcgtcttt
    12181 cggggcgttc ctcgacccgg tggccgacaa gctcatggtc agcgcggcct tgctgatcct
    12241 gctgcatctt gggcgtgtcg acatcctcat cgccctggtc atcgtggggc gtgaaatcgc
    12301 gatttccgca ctcagggaat ggatggcaaa aatcggcgcc agcggcagcg tggctgtgca
    12361 ttggctgggg aaattcaaga cagcagccca gatggtcgcg attccgtgcc tgttgtactg
    12421 gcagcctgtt cagggcgtgt cggcccgggt agtcgggcag gtgctcatcg tcatcgcggc
    12481 catcctgacc gtatggtcca tgtgctatta cctgcgccgc gcctggcccg agatccgcga
    12541 tcggtcccaa gccttctgac gggtcttatt gacgtggcgt gcggctcagg ctgacatcga
    12601 tggccgagct ggcgcgccac gggttgatgt ccagcccccc ccgccgggtg taccgggcat
    12661 agaccagcag ccattccggc tggcaggcgc gccatatgtc gcagtacatc tgttccacgc
    12721 agtgctcgtg gaattccttg tgccggcgca gcgaaacaat gtactggagc aggctggccg
    12781 ggtcgatcct ccgtccccgg taacgtactt gcacgctgcc ccaatcgggc tggccggtga
    12841 ccgggcaatt tgacttgagc agatcggaca ccagcgtttc gtccaccacc gccgcgtccg
    12901 gcagacacct cagcaaccct ggaacgggct ggtagtgctg gatctcgaca tccagatcgt
    12961 cgatgttggt gccggaaagc ggctgcggga cttccgaacg attggcgttc agacgatcca
    13021 gccagacccg caccggcgct ccggcggcgt ggctcagatc ctgctcaaca aggtgccgca
    13081 cggcctccgt gccgtcgaat cgcgtttccg tgaacgagtt cagatatagc ttgagggatt
    13141 tcgactcgat caggcaaggg ctggattccg gaaacacgaa ccgcgccgtg cccaccatgg
    13201 gtttgccccg ggggttgagc caggagagtt cgtaagcggt ccagatgtcc gccccggtcc
    13261 agggtggcaa cggcgccagg gccgcgcggt tcgcctcgcg cgcgatggga aacagcagcg
    13321 cgggatcgta gtggtcgggg taacggatat cgtgacccag cggggcattg gccaggccgg
    13381 attcgttcat tgcgcgtgtc ggcgggtgtt gagttggatc atgcattgta ctgccgccga
    13441 tcacgcgttt tcgtatggcg gaatttagat tttgcattat gaaaattaat tattgcaacg
    13501 cacgatatta tttttcacta acgaaaaatc aatttcatat tacaaaataa aattgataaa
    13561 taattgatcc ataacaataa tattttttgt cttatataag acatatgagt ttgtcgcaga
    13621 gcgtcattgg cgtagacttt tttcaacggc cgcccttgtg ggtcccgact gatttttcaa
    13681 ctccactcta tgaaaagggg catgaccatg acgacacgtg aaaccgaaat ccgtcgactg
    13741 caggaaaact gggcggaaga tccgcgctgg caaggcgtca aacgcggcta tggcgccgag
    13801 gaagtcgtgc ggctgcgcgg ctccgtgccg gtcgagcaca cgctggcgcg ccggggcgcc
    13861 gagaagctgt ggggcttgct gggctccgaa ccgttcgtca attcgctcgg cgcactgacc
    13921 ggcaatcagg ccatgcagca ggtcaaggcg ggactcaaag ccatctacct gtcgggttgg
    13981 caagtggccg gagacgccaa cggcgctggc gaaatgtatc cggatcagtc gctgtatccc
    14041 gcgaactccg tgccacaggt cgttcggcgt atcaacaatg ccttgacgcg ctgtgatcag
    14101 attcaatgga tggaaggcgc caatccaggc gatcccggct atgtggatta cttcgcgcct
    14161 atcgtggccg acgccgaagc gggtttcggc ggtgtgctca atgcgttcga gttgatgaag
    14221 tccatgatcg aggctggcgc ggcgggggtg catttcgagg atcaactggc ttcggtgaaa
    14281 aaatgcgggc atatgggcgg aaaggtgttg gtgcccacgc gtgaagcggt agcgaaactg
    14341 gtgtcggcgc ggctcgccgc cgacgtgctc ggtgtgccga cggtcttgct ggcgcgcacc
    14401 gatgccgatg ccgccaatct gctgaccagt gacgtcgacg agaatgatcg ccccttcatc
    14461 acgggcgaac gcaccgccga gggcttcttc cagacgcggg cgggcattga ccaggcgatc
    14521 tcacggggac tggcctatgc gccgtacgcc gatctgttgt ggagtgaaac ctccacaccg
    14581 aacctggagt atgcccgcaa gttcgcggag gccatccacc gccgctttcc gggcaaactg
    14641 ctggcctata actgttcgcc gtccttcaac tggagaaaga atctggacga tgccacgatc
    14701 gccaagttcc agcgtgagct cggcgccatg ggctacaagt tccagttcat caccctggcg
    14761 ggcttccatg cgctcaatta cggtatgttc gaactggctc acggctatgc gcggcgtcag
    14821 atgagcgctt tcgtggagct tcagcagaag gagttcgcgg cggccgatct gggcttcacg
    14881 gcggtcaagc atcagcgtga ggtgggtacc gggtatttcg acgccgtgac ccagaccatc
    14941 gaaggcgggc ggtcgtccac cacggcattg tccggttcca ccgaggaggc gcagttcgag
    15001 gacgacggcg ggcagaccgg gctcagggtt gcttgaggca acaccattcc gccgctttcg
    15061 gcaaagcggc ggaatggctg cgtgaacccg ggcctaatcc ttgaatatga gggcgtgcag
    15121 ctttgcccgc acctgggcca ggtcggctgg cccgatgcga ccgtggaagc tgatcttgct
    15181 gtcggcccaa cctaggctct tgacctggtc ggacaggacc gcgccgccct cgatgggcac
    15241 ttcgaaaggg tagcccttga tgctgggcgt gatggggcag cagaccatca ggccggttcg
    15301 cttgttatag gcttgcgggc tgagcaccat ggctggtttg taggacgaag aggcgccgcg
    15361 gcccctggcc tcgaagtgga catggatgat gtcgcccgtg tcggggatat agcgcttgct
    15421 catggtgggc tcgatcagtc gccggcggcg ccgaagtcga cctcagggtg cagattacga
    15481 gccttgacgc cgcttaatag ttgagacaat acatattcac cggtacgcac cggctcgatg
    15541 aggatcgttc cgcgttccac gcggatgtcg atcatgtctt caaggcccag tttcagggtt
    15601 ttgatcattg aggacggcag gcgcacggcc gcactgttgc cccatttctt gatgacgcca
    15661 cgcatggacg actcctgtgt ctggcgtggc cagcaggttt ctcagctggc cggcctgtgg
    15721 tattcaaggg gctgtgatca cccgcgttcg cggctttgtc gcgaaccggc gaccgacagt
    15781 gctagcatac cagtcgcatg cgtcgcaaac aataatctcg aataataaac tgtttccata
    15841 tcccgaaagg cgtttaatag acggatggcg caaaatggtg tggtgtctgg gccactcaac
    15901 ggtttgaacg ccggcttggt gtgtggcgcc atgcgggttg gatcggcggc accgccttga
    15961 aattcgctcg atcgcccgca cgtattctgt attcggacga ccgtcccaga ccgatactca
    16021 gagtgaagca accatgcgat tcgacaaact gaccacacga ttccagcagg cgattgcgga
    16081 tgcgcaaagc ctggctgcca gacacgacaa tccctacatc gagccggtgc atgtgctggc
    16141 cgccttgttg tccgacgcgg acagcggcgc cggcagcctg ttggcgcgcg ccggcgttgg
    16201 cgtgaaccgg ttggtatcca gtgtgaacac ggtgattgcg gcgctgccgc aggtggctgg
    16261 gtcggaagag aatcttcaag tcagccgtga cctgaacacg ttgctcaccc gcaccgacaa
    16321 ggaagcggcc ggccgtggtg acacgtatat tgccagcgaa ctgttccttc tggtgctggc
    16381 cgatgacaag ggtgaagcag ggcggctgct gcgcgaggcg gggctgcgca agaacgccct
    16441 ggagaccgcc atcgacgcgg tgcgcggtgg cgcgcccgtg gcggactccg aaggcgaggc
    16501 caaccgcgag gcgctggcca agtacacatt ggatctcacg gaaaaggccc gtcagggaaa
    16561 actcgacccg gtcatcggcc gggatgacga gatccggcgc acgatccaga tattgcagcg
    16621 ccgcacgaaa aacaatcctg tgctgatcgg cgaacccggc gtgggcaaga ccgccatcgt
    16681 cgaagggttg gcgcaacgta tcgtcaatga tgaggtgccc gaaaccctga aaggcaaacg
    16741 ggtcttggcg ctggatctgg cggccctgct ggccggggcc aagttccgtg gcgaattcga
    16801 agagcgactc aaggcggtgc tcaaggagct cgcccaggac gatggccaga ccatcgtctt
    16861 catcgatgag ctccacacca tggtgggcgc cggcaaggcc gagggcgcca tggacgccgg
    16921 caacatgctc aaacccgcgc tgtcgcgcgg cgaactgcat tgcgtggggg ccaccacgct
    16981 cgac
//
LOCUS       DASTNK010000138        21185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1037205, whole genome shotgun sequence.
ACCESSION   DASTNK010000138 DASTNK010000000
VERSION     DASTNK010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 21185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21185
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037205"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            419..1523
                     /gene="prfB"
                     /locus_tag="VFR20_10265"
     CDS             join(419..499,501..1523)
                     /gene="prfB"
                     /locus_tag="VFR20_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417196.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU0230945.1"
                     /translation="MEAERQNQLAARLADYAQRESDLRGYLDYDAKSERLIVVNAELE
                     SPDVWNDPKHAQELGREKKSLENIVLTLSSLGTGLADAGELFELASLDDDDATLHAIE
                     ADCDAMGERIEALEFRRMFSNPADPLNCFLDIQAGAGGTEAQDWASMLLRQYLHYCER
                     KGFKAEVLEESEGEVAGIKSATIKVEGDYAFGHLRTESGVHRLVRKSPFDSANGRHTS
                     FASVFVYPEVDDSIEIDINPADLRIDTYRASGAGGQHINKTDSAVRITHTPSGIVVQC
                     QNDRSQHRNRAEAMQMLKSRLYELELRQRMAEQQKLEDAKSDVAWGHQIRSYVLDQSR
                     IKDLRTNVEISNTQKVLDGDLDPFIQASLKQGV"
     gene            1577..2326
                     /locus_tag="VFR20_10270"
     CDS             1577..2326
                     /locus_tag="VFR20_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0230946.1"
                     /translation="MSISTVAIVTGASAGLGQALALGLMGPDTHLLTLSRRHDAALDA
                     HATASGCTVQQIQVDLSNPAATEVGAEQVIGNLPRSAQRYLLINNAGMVDPVAPTAQL
                     SSAARITAALSLNVTSVMLLAAAFLRNTPTGADRRILNISSGAGRSAMPGWGVYCASK
                     AALDRYTEVLAAENPTVRTVSLAPGVVNTGMQAHIRASSADNFPNVQRFIAMHDEGKL
                     AEPAAVAAKILNYLTSPDFGKTVLDDIRHHD"
     gene            2337..3863
                     /gene="lysS"
                     /locus_tag="VFR20_10275"
     CDS             2337..3863
                     /gene="lysS"
                     /locus_tag="VFR20_10275"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU0230947.1"
                     /translation="MTDTTVTTQTQDTNHLIAERRAKLAKLREHGPAYPNDFRPIDAA
                     GDLHTAFDEHDKDALLAQGRHASVAGRMMLKRVMGKASFATLQDRSGRIQIYLDRGTL
                     GDEVYAQFKTWDIGDILGVEGVVFKTNKGELSIHADRIRLLSKSLRPLPDKFHGVADQ
                     ELRYRQRYVDLIMTEATRDTFMTRGRIIGGIRQFMLDAGFLEVETPMLHPIPGGATAK
                     PFATHHNALDMEMFLRIAPELYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMME
                     FYAAYTDYRWLMDFTESLIRGVATTACGSASLTYQDKPLDLTRPFDRLTIVEAILKYD
                     SRYTPAQLGDEGFLRQELKRLGADVDGPTLGHAGRGALQLALFEETVEAKLWHPTFII
                     DYPTEVSPLARESDTQPGITERFELFATGREIANGFSELNDPEDQAERFRAQVAAKDA
                     GDEEAMYYDADYIRALEYGMPPTGGCGIGIDRLVMMLTDSPSIRDVILFPHLRREEPA
                     "
     gene            complement(3933..4256)
                     /gene="iscA"
                     /locus_tag="VFR20_10280"
     CDS             complement(3933..4256)
                     /gene="iscA"
                     /locus_tag="VFR20_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEU0230948.1"
                     /translation="MAITLTQRAAEHIGRHLAKRGTGVGLRLGVKVSGCSGLAYKLDY
                     VDQPGAEDQVFEQFGVKVFIDPKSLPFLEGTQIDYSRDGLNEGFKFDNPNEKASCGCG
                     ESFTV"
     gene            complement(4260..4643)
                     /locus_tag="VFR20_10285"
     CDS             complement(4260..4643)
                     /locus_tag="VFR20_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEU0230949.1"
                     /translation="MAYSAIIADHFEHPRNVGTLDANDPRVGTGLAGSPAQGDVIRLQ
                     IRVDGSGIIEEARFKTYGSGAAIATSSLATEWLRGKTLAQAVAIDNMAIARELQLEPI
                     KLHCAMLAEDAIAAAVRNYQEKHQG"
     gene            complement(4696..5907)
                     /locus_tag="VFR20_10290"
     CDS             complement(4696..5907)
                     /locus_tag="VFR20_10290"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEU0230950.1"
                     /translation="MPDRLVYLDNSATTPVDPRVVEKMVPWLYERFGNPASNSHALGW
                     DAEEAVEHARSQVAQLVNADPREIIWTSGATESDNLALKGAAAHYAGRGRHIVTVKTE
                     HKAVLDTCHELERQGFEVTFLDVDPDGLVDPGVFREALRPDTILASVMLVNNEIGVIQ
                     DIAAMGAACREKGVVFHVDAAQATGKVAIDLQALEVDLMSFSAHKTYGPKGIGALYVR
                     RKPRVRLEAQIHGGGHERGLRSGTLATHQIVGMGEAFELARLEMARENAGLRVLRDRL
                     WAGLSQLDDVCLNGSMTHRVAHNLNVSFSYVEGEALIMGLKGLAVSSGSACTSASLEP
                     SYVLRALGRRDELAHSSIRFTLGRFSTPEDVDFAVREVTRQVNRLREMSPLWEMAKQG
                     VDVDTLTWPTH"
     gene            complement(5924..6436)
                     /locus_tag="VFR20_10295"
     CDS             complement(5924..6436)
                     /locus_tag="VFR20_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcription factor"
                     /protein_id="HEU0230951.1"
                     /translation="MRLTTKGRFAVTAMIDLALRHQSGPVTLAAISERENISLSYLEQ
                     LFGKLRRHELVDSMRGPGGGYTLARLARNITVADIIFAVDEPLDATSCGGKENCGVGR
                     ITSTGKCMTHELWSTLNRKMIDYLDSVSLQDLVDQQRMRLLQESTQQAPIRLHRDVKM
                     TSPMSTPTTA"
     gene            complement(6712..7203)
                     /locus_tag="VFR20_10300"
     CDS             complement(6712..7203)
                     /locus_tag="VFR20_10300"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEU0230952.1"
                     /translation="MMTKVLFVCMGNICRSPSAEGVFRRMVDDAGLSAVVGVDSAGTH
                     SFHIGEAPDARAQAAARKRGYDISQCVARQVVPADFREFDLILAMDWENLSALQQQCP
                     KIYQHKLMLLMRFANEFEEATVPDPYYGGPEGFGKVLDYIEDACQGVLELVRKRATQY
                     QAA"
     gene            complement(7595..9697)
                     /gene="uvrB"
                     /locus_tag="VFR20_10305"
     CDS             complement(7595..9697)
                     /gene="uvrB"
                     /locus_tag="VFR20_10305"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEU0230953.1"
                     /translation="MAAAINAPADSPADAAGGPAVASPAASTGPGPGFIEFPDSPFHL
                     YQPYHPAGDQPQAIDSLVEGVDDGLMYQTLLGVTGSGKTYTMANVIARTGRPALVLAP
                     NKTLAAQLYAEMREFFPNNAVEYFVSYYDYYQPEAYVPSRDLFIEKDSSINEHIEQMR
                     LSATKSLLERPDTIIVGTVSCIYGIGNPGDYHAMVLTLRTGDHIARRELLARLVAMQY
                     ERNDVDFVRGTFRARGEIIDVFPAEHAELAVRITMFDDEIESIDLLDPLTGKLKQKLL
                     RFTVFPSSHYVTPRDTVLRAIGTIKEELRDRLAWFTGQGKLVEAQRLEQRTRFDLEML
                     QELGFCKGIENYSRHLSGALPGEPPPTLIDYLPANALMFIDESHVTMGQLGGMYRGDR
                     SRKETLVQFGFRLPSAVDNRPLKLEEFEQRMRQCVFVSATPSDYEKNHADSVVEQVVR
                     PTGLVDPEVDIRPAKTQVDDLLGEIKKRVTVQERVLVTTLTKRMAEDLTDYLGENGVK
                     VRYLHSDIDTVERVEILRDLRLGVFDVLVGINLLREGLDIPEVSLVAILDADKEGFLR
                     SERSLIQTIGRAARNLNGRAILYADRITDSMRRALDETDRRRNKQIAFNKEHGITARG
                     VRKAVRELIDGVMAQAEPDSALDAVPLEVLADDKALAREIRRLETQMMDHARNLEFEQ
                     AAAARDALNALKKRALLS"
     gene            9877..11076
                     /locus_tag="VFR20_10310"
     CDS             9877..11076
                     /locus_tag="VFR20_10310"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HEU0230954.1"
                     /translation="MTSVFDAVELAPRDPIFGLNEQFNADNRPDKVNLGVGVYCDDHG
                     RIPLLDSVHKAEVARVQAAAARGYLPIDGIAAYNKGAQALLLGKDSRVLADGRAVTMQ
                     ALGGTGALKIGADFLKTLLPNAQVAISEPTWANHRAIFEGAGFKVSTYAYYDADTHGL
                     NFDGMLASLRALPAGSIAVLHACCHNPTGVDLTLDQWKQIAQVVKDSRLVPFVDIAYQ
                     GFGDGLDEDAAAVRLLADLDLTMLVSSSFSKSLSLYGERVGALTVVVSGKDEGTRVLS
                     QVKRVIRANYSNPPTHGGMLVASVLNTPELRTLWETELAGMRNRIRDMRNQLVEKLAA
                     QGVTRDFSFVREQRGMFSYSGLTSAQVDRLRTEYGIYAVGSGRICVAALNTRNIDYVA
                     KAIAQVL"
     gene            11141..11806
                     /gene="lexA"
                     /locus_tag="VFR20_10315"
     CDS             11141..11806
                     /gene="lexA"
                     /locus_tag="VFR20_10315"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041937182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEU0230955.1"
                     /translation="MAIKLTARQQQVLDLIRTEIARTGFPPTRAEIARALGFKSANAA
                     EDHLKALARKGAIELTANASRGIRPLICPGVPAKATSQMAEALQAGLAQLLLPLVGRV
                     AAGSPVLAAEHVEREVGVEASLFSQTPDYLLRVQGSSMRDAGILDGDLLAVKKSADAR
                     NGQIVVARLGDDVTVKRLSRSGSHITLLPENPDFSPIHITAADDFSLEGIAVGLIRAT
                     PLH"
     gene            complement(12307..14757)
                     /gene="lon"
                     /locus_tag="VFR20_10320"
     CDS             complement(12307..14757)
                     /gene="lon"
                     /locus_tag="VFR20_10320"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEU0230956.1"
                     /translation="MSASQILSSEPMDLPLLPLRDVVVFPHMVIPLFVGRPRSIKALE
                     LAMEAGNNIMLVAQKSAGKDDPTPEDLYDIGCAASILQMLKLPDGTVKVLVEGLQRAS
                     IQHVSDAQTHFVANVVPIAPDASQNSESEALRRTVVAQFEQYVKLNKKIPQEILTSLS
                     GIDDAGRLADTISAHLPLKLEQKQKMLEIIDTSGRLENLLSQLEAEIDILQVEKRIRG
                     RVKKQMEKSQRDYYLNEQVKAIQKELGEGEEGADIEELEKKIEAAHLPKEARKKADAE
                     LKKLKLMSPMSAEATVVRNYIDTLISLPWRKKSRVNNSIANAEEVLDADHYGLEKVKE
                     RIVEYLAVQQRVDKLKAPILCLVGPPGVGKTSLGQSIARATNRKFVRMALGGVRDEAE
                     IRGHRRTYIGSMPGKILQNMTRVAVRNPLFLLDEIDKIGADFRGDPSSALLEVLDPEQ
                     NHTFQDHYVEVDFDLSDVMFVATSNTLNIPPALLDRMEVIRLSGYTEDEKVHIAFDHL
                     LPKLMKNNGVKEGELSIDESAIRDIVRYYTREAGVRALEREISKICRKTVKRMLGAAA
                     AGGDAKRETIKVDADNLNDFLGVRRFDFGHAGKENKVGQVTGLAWTEVGGELLTIEVA
                     DMPGKGVVLRTGSLGEVMKESVEAARTVVRARAQRWGIPDKAFERRDLHVHFPEGATP
                     KDGPSAGIGLTTAMVSALTGIPVRADVAMTGEITLRGEVLPIGGLKEKLLAAHRGGIK
                     VVMIPEENVKDLAEIPDNVKNSLEIIPVRWIDRVLEVALQHMPTPLSDEEVARLAAES
                     VANAKPSGGEPSSELFKH"
     gene            complement(14913..16223)
                     /gene="clpX"
                     /locus_tag="VFR20_10325"
     CDS             complement(14913..16223)
                     /gene="clpX"
                     /locus_tag="VFR20_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU0230957.1"
                     /translation="MAEKKGSADDKVLHCSFCNKSQHEVRKLIAGPSVFICDECIDLC
                     NDIILEETRDAARDAIRNELPTPVEIKTFLDGYVIGQEAPKRSLAVAVYNHYKRVRYT
                     EVKGDDVELSKSNILLVGPTGSGKTLLAQTLARMQNVPFVMADATTLTEAGYVGEDVE
                     NIIQKLLQNCNYDVEKAQRAIIYIDEIDKISRKADNPSITRDVSGEGVQQALLKLIEG
                     TVASVPPQGGRKHPNQDFVQVDTTNILFICGGAFDGLEKIIRDRTEKSGIGFSASVRA
                     KTEKGIGELFAEAEPEDLIKFGLIPELVGRLPVVATLEELEEDTLVRILTEPRNSLIK
                     QFQKLFQMEEVELDVRPQALSAIARRALKRRTGARGLRSIVEQSLLTTMYELPSQKNV
                     QRVVVDENAVTGKGSPILIYADQEEARAADSRSGQSKKLKDAAA"
     gene            complement(16321..16980)
                     /gene="clpP"
                     /locus_tag="VFR20_10330"
     CDS             complement(16321..16980)
                     /gene="clpP"
                     /locus_tag="VFR20_10330"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEU0230958.1"
                     /translation="MSHFTDFYASMHGGDSVRPSGLGYVPIVIEQSGRGERSYDIYSR
                     LLRERVVFLVGPVEDQSANLIVAQLLFLESENPDKDISLYINSPGGVVYSGMAIFDTM
                     QFVKPDVSTLCTGFAASMGAFLLAAGAKGKRYSLPNSRIMIHQPSGGAQGQASDIQIQ
                     AREILSLRERLNSILAERTGQPLERIAEDTERDNYMSAADAVSYGLIDDVLVSRADTK
                     K"
     gene            complement(16992..18302)
                     /gene="tig"
                     /locus_tag="VFR20_10335"
     CDS             complement(16992..18302)
                     /gene="tig"
                     /locus_tag="VFR20_10335"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU0230959.1"
                     /translation="MQPVVETLSGLERRVDLVVPLAEVEKQVQAQLKRVARTAKVQGF
                     RPGKAPLAMIERSHGPGIRYDVINTEVGRALDKAIQDANLRVAGTPNLEPKTEGVEEG
                     IMAFSATFEVYPEITAPDIAALKVTRANVEVSDADVQHTIDILRKQRANYEAREGRAA
                     QDGDRVTLDFAGTIDGVPFDGGKAEKFPFVLGQGRMLPEFEAAVQGMKAGENKTFPLE
                     FPADYGGKEVAGKKAEFAITVTEVAEAVLPAVDAEFAKSLGQAEGDVDKLLAEVRGNV
                     DREVKARAQARTKGSVMDALVGACSFDVPKALVQSDTESRIAAAREDLKQRGVPNAES
                     VPIPEDMFKAESERRVKLGLLVSELVDKADLKAKPEQVRTRIEDFAKSYEQPAQVVAY
                     YLSDRQRRAEIEAIVLEDNVVDYVLSKAQVTDEQVAFDELMGTA"
     gene            18479..19669
                     /locus_tag="VFR20_10340"
     CDS             18479..19669
                     /locus_tag="VFR20_10340"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU0230960.1"
                     /translation="MPKPLDGVRVLELGQLIAGPFAAKMLGEFGAEVIKIEPPETGDP
                     LRKWRLLHNGTSVWWAVQSRNKKSVTLDLREPEAQDAVRKLAATADVVIENFRPGTLE
                     GWGLGWDALHALNPRLVMLRVSGYGQTGPYRDRPGFGVVGEAMGGLRHLSGEPGRPPV
                     RVGVSIGDSLSALHGVIGVLLALRQRDQNGGDGQCVDVALYESVFNMMESLLPEYAVF
                     NEVRQPAGSSLPGITPSNAYLCADGKYALIAGNGDSIFKRLMVAIGRPEVAENPAYAH
                     NDGRARDAVTIDGIIGDWARGHDLEHVLHVLHEARIPAGKVYDIADICADPHYQARGM
                     ILDSTLDDGTPVKLPGIVPKLDATPGDVRRGAPTLGQDTDQVLGELGIDAKTRQGWRE
                     RGII"
     gene            19809..20531
                     /locus_tag="VFR20_10345"
     CDS             19809..20531
                     /locus_tag="VFR20_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0230961.1"
                     /translation="MLIDNRLPRYQRLRDELITLITAGQWRPGETIPTEQSLAQTHNV
                     AIGTVRKAVDLLVAEGLLERFQGRGTFVRRASFDNSLFRFFRFQTRQGERRIPESRIL
                     RREVVVAPSAVATALQVAPGTEVIQMSRLRLANDVPLLAEEIWLPHDRFTAFLQLDLT
                     SIGNLLYPVYEAQCNQIVASATETLTVEAINPMYARLLKIAPGTPGVVIERLAYGYDR
                     QPLEWRRSRGPASEFIYQVEIR"
     gene            20600..>21185
                     /locus_tag="VFR20_10350"
     CDS             20600..>21185
                     /locus_tag="VFR20_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0230962.1"
                     /translation="MFSWFKEVSSNERRTFWACFGGWALDALDVQMFSLVIPAIIAEW
                     SISRTDAGVVSGATLVASALGGWVAGALSDRYGRVRILQWTVAFFSVFTFLSAFAQSY
                     PQLLILKSLQGFGFGGEWAAGAVLMAETIRTQHRGKAMGSVQSAWAVGWAAAVLLYAL
                     MFSLFPGDMAWRLMFALGILPGLLVLYIRRGVQEP"
BASE COUNT         4080 a         6831 c         6266 g         4008 t
ORIGIN      
        1 gccacgtagg cccccaccag agacatgcag agccccaggc agccatacgc cagcatgcgg
       61 ctcggattct gggtcggatt catggacacg gcatattgaa tagggggcca cacgcccggc
      121 ccgtggcaac aaaagcgggc atgccggagg ctggcgccca acgatagcac aggcggcaca
      181 agggggttgt ggcgacgccc tgtggggttc gggggaaatg cctcttcgcg acccgccaca
      241 ggccccatgg acgcgtcggg ttaaaatcct gtgatcaacg cgcaaagtcc acagcgacat
      301 tcccgaaaca ccgcattcgt gcgccacgtc cgtgacgccg cctgatgcgg aacaacccgg
      361 aaccatcact gcgaccctgc ccgaatccat cacccccctc atcatcaccg gacacatcat
      421 ggaagccgaa cgtcagaacc aactcgccgc ccgcctggcc gactacgcgc aacgcgagtc
      481 cgacctacgg gggtatcttt gactacgatg ccaagtccga acgcctgatt gtcgtcaacg
      541 cggaactcga atcccccgac gtctggaacg acccgaagca cgcacaagag ctcggccgcg
      601 aaaagaaaag cctcgaaaac atcgtcctga ccctttcctc gctcggcacc gggctggccg
      661 acgccggcga actgttcgag ctcgccagcc tcgacgatga tgatgccacg ctgcacgcca
      721 tagaagccga ctgcgacgcc atgggcgagc ggattgaggc tcttgagttc cgccgcatgt
      781 tctcgaaccc ggcggatccc ctgaactgct tcctcgacat ccaggcgggc gcgggcggca
      841 cggaagccca ggactgggcc tccatgctgt tgcgccagta cctgcactat tgcgaacgca
      901 agggtttcaa agccgaagtc ctggaagagt ccgagggcga ggtggcgggc atcaagtcgg
      961 ccaccatcaa ggtggaaggc gattacgcct tcggccacct gcgcaccgaa tccggcgtgc
     1021 atcgcctcgt gcgcaagagc ccgttcgact ccgccaacgg ccgccatacc tcattcgcca
     1081 gtgttttcgt ctatccggaa gtggatgact ccatcgaaat cgacatcaat ccggccgacc
     1141 tgcgcatcga tacctaccgt gccagcgggg cgggcggcca gcacatcaac aagaccgact
     1201 cggcggtgcg catcacgcac actcccagtg gcatcgtcgt gcaatgccag aacgaccgtt
     1261 cccagcaccg caaccgcgcc gaggccatgc agatgctgaa gtcgcggctc tacgaactgg
     1321 aactgcgcca acgcatggcc gagcagcaga aactcgagga cgcgaaatcc gatgtggcct
     1381 ggggccatca aatccgctcc tatgtgctgg accagagccg catcaaggat ttgcgcacga
     1441 acgtggagat ctcgaacacc cagaaggtgc tggacggcga cctcgacccc ttcatccagg
     1501 ccagcctgaa gcaaggggtc tgacacgcca tgggccttca gccctgctcc catcaacaca
     1561 accacaagga ttcgcgatga gcatttcgac cgtggccata gtgaccggcg catcggccgg
     1621 tctgggccag gctctggccc tgggcctgat gggccccgat acccatctgt tgaccctgtc
     1681 acgccgccat gacgccgcgc tggacgcgca cgcgacggca tcgggctgca ccgtgcagca
     1741 aatccaggtc gatctgtcca atcccgccgc aacggaagtc ggcgccgagc aagtcatcgg
     1801 caatctgccg cgctccgcgc agcgctatct gctcatcaac aatgccggca tggtggaccc
     1861 cgtggcgcca acggcccagc tgagcagcgc ggcgcgcatc accgcggcgt tgagcctgaa
     1921 cgtgaccagc gtcatgctgc ttgcggccgc gttcctgcgc aatacgccga ccggggccga
     1981 tcgccgcatc ttgaacatct cgtcgggcgc cggacgctcg gccatgccgg gctggggcgt
     2041 gtactgcgcg tccaaggccg ccctggatcg ctacacagag gtattggcgg ccgaaaaccc
     2101 caccgtgcgc accgtttcac tggcccctgg cgtagtcaac accggcatgc aggcccacat
     2161 ccgggccagc agcgccgaca acttccccaa tgtgcaacgg ttcatcgcca tgcacgacga
     2221 aggcaaactc gcggaacccg ccgccgtcgc ggccaagata ttgaattacc tgaccagccc
     2281 cgatttcggc aagaccgtac tcgacgacat tcgtcaccac gactgacgct tcatccatga
     2341 ccgacaccac cgttaccacc cagacccagg acaccaacca cctgatcgcc gaacgccggg
     2401 ccaagctggc caagcttcgg gaacacggac cggcgtatcc caacgacttc cgtcccatcg
     2461 atgccgccgg cgacctgcac acggccttcg atgagcacga caaggatgcg ctgctcgcac
     2521 aggggcggca tgccagtgtg gccggacgca tgatgctcaa gcgcgtcatg ggcaaggcca
     2581 gtttcgccac cctgcaggat cgttcggggc gcatccagat ctatctggat cgcggcaccc
     2641 tgggcgacga ggtctacgca cagttcaaga cctgggatat cggtgacatc ctgggcgtgg
     2701 aaggcgtggt gttcaagacc aacaagggtg aactgtccat tcacgcggat cgcatccgcc
     2761 tgctgtccaa gtcgctgcgc cccctgccag acaagttcca tggtgtcgcc gaccaggaac
     2821 tgcgctaccg ccagcgctac gtcgacctga tcatgaccga ggccacccgc gacaccttca
     2881 tgacacgcgg caggatcatc ggcggcattc gccaattcat gctggacgcg ggtttcctgg
     2941 aagtcgaaac tcccatgctg cacccgattc ccggcggcgc cacggccaag ccgttcgcca
     3001 cgcaccacaa cgcgctcgac atggaaatgt tcctgcgcat cgcgcccgaa ctttacttga
     3061 agcgcctgat cgtcggcggc ttcgagcgcg tattcgaaat caaccgcaac ttccgcaatg
     3121 aaggggtgag cccgcgccac aaccccgagt tcaccatgat ggagttctac gcggcgtata
     3181 ccgactaccg ctggctgatg gatttcaccg aatcgctcat tcgtggcgtg gccaccaccg
     3241 cctgcggcag cgcgtcgctg acctatcagg acaaaccgct ggacctgacc cggccattcg
     3301 accggctgac gatcgtggag gccatcctca aatacgacag ccgctatacg cccgcccagt
     3361 tgggcgacga aggcttcctg cgccaggagc tgaagcgcct cggcgccgac gtcgacggcc
     3421 ccacgctggg gcatgcgggg cgcggcgcgc tgcaactggc gctgttcgag gaaacggtgg
     3481 aagccaagct ctggcacccc acgttcatca tcgactaccc caccgaggtg tcgccgctgg
     3541 cccgagagtc ggacacccag cccggcatca ccgaacgctt cgagctgttc gccacgggac
     3601 gggaaatcgc caacgggttc tcggagctga acgatcccga agaccaggcc gaacgcttcc
     3661 gcgcccaggt ggcggccaag gatgccggtg acgaagaagc catgtattac gacgccgatt
     3721 acatccgcgc gctggaatat ggcatgccac ccaccggcgg ctgcggcata ggcatagatc
     3781 gcctggtcat gatgctgaca gacagcccca gcatccgcga cgtgatcctt ttcccgcatc
     3841 tgcggcgcga agaacccgcg tgatcgccac cggccaacac gacaaaagcg gccccgcttc
     3901 aaaggaagcg gggccgcttt ttttgtggcg ggtcacaccg tgaacgattc cccgcagccg
     3961 caggaagcct tttcgttggg attatcgaac ttgaagcctt cgttgagccc gtcacgggaa
     4021 tagtcgatct gcgtgccttc caggaagggc aggcttttcg ggtcgatgaa caccttgacg
     4081 ccgaactgct cgaacacttg gtcttccgcg cccggttgat cgacgtaatc gagcttgtaa
     4141 gccagccccg agcagccaga gaccttcacg cccaggcgca gcccgacgcc tgtgccgcgc
     4201 ttggcgaggt ggcggccgat gtgttcggcc gcgcgttgcg taagagtgat tgccatgatt
     4261 cagccctgat gtttttcctg ataattgcgc acggccgccg cgatggcgtc ttccgccagc
     4321 atggcgcaat gcagcttgat gggttcgagt tgcaattcac gcgcgatggc catgttgtcg
     4381 atggccacgg cctgggccag cgttttgccc cgcagccatt cggttgccag cgagctcgtc
     4441 gcgatcgcgg cgccagaacc gtacgtcttg aaacgtgctt cttcaatgat acccgatccg
     4501 tcaacccgta tctggagacg gatcacgtcc ccctgggcgg gtgagccggc caggcctgtg
     4561 cccacccggg gatcgttggc atcaagcgtg ccgacgttgc gggggtgttc gaaatgatcg
     4621 gcgattatcg cgctgtatgc cacggctgct ctccggctct ctacttaggg tcttgttcag
     4681 attgagaaga cggcgtcaat gcgtcggcca tgtcagcgtg tcgacgtcca cgccctgctt
     4741 ggccatttcc cacaacggcg acatctcgcg caatcgattg acttggcgcg tgacttcacg
     4801 caccgcgaaa tcgacgtctt cgggcgtgct gaagcgaccc agcgtgaagc ggatggagct
     4861 gtgcgccagt tcgtcgcgcc gacccagcgc acgcagcacg taagaaggct ccagactggc
     4921 cgaggtgcag gccgagccac tggaaaccgc caagcccttg agccccatga tgagcgcttc
     4981 gccctcgacg tagctgaaac tgacattcag gttgtgcgcc acacggtgcg tcatgcttcc
     5041 gttcaggcaa acgtcgtcga gctgggacaa gcccgcccac aaccggtcgc gcaacacgcg
     5101 caagccggcg ttttcccgcg ccatttcgag gcgtgccagt tcgaacgcct cgcccatgcc
     5161 gacgatttga tgcgttgcca gcgtgcccga gcgcaagccg cgctcatggc caccgccgtg
     5221 gatctgcgcc tccaggcgca cccgcggctt gcggcgcaca tagagcgcgc cgatgccttt
     5281 cggaccatac gtcttgtgcg ccgaaaacga catcagatcc acctcaagcg cttgaagatc
     5341 gatggcgacc ttccccgtgg cctgagccgc atccacatgg aaaacaacgc ctttttcgcg
     5401 gcatgccgcc cccatggcgg caatgtcctg gatgacgccg atttcattgt tgacgagcat
     5461 gaccgatgcc agaatcgtat cgggccgcaa agcctcgcga aacacgccag ggtccaccaa
     5521 gccgtcggga tccacatcga ggaacgtgac ctcgaacccc tgccgctcca gttcgtggca
     5581 ggtgtcgagc acagccttgt gctccgtctt gacggtgacg atgtgccgcc cacggccagc
     5641 gtaatgcgcg gccgccccct tcagcgccaa gttgtcggac tcggtggcac ccgatgtcca
     5701 gatgatttcg cgcgggtccg cattgaccaa ttgcgcgact tgcgaacgcg cgtgctcgac
     5761 cgcctcttcg gcgtcccatc cgagcgcatg gctgttggac gcgggattgc cgaatcgctc
     5821 gtagagccaa ggcaccattt tttcgacgac gcgcgggtcg acgggggtgg tggccgagtt
     5881 gtcgagatag acgaggcgat cgggcatggt ggctccgtga aattcaggcc gtggtcggcg
     5941 tggacattgg tgatgtcatt ttaacgtcgc gatgcaatcg tatcggcgct tgctgagtgg
     6001 actcctgcaa cagccgcatg cgctgttggt cgaccagatc ctgcagcgac accgaatcca
     6061 ggtaatcgat cattttgcgg ttgagcgtgg accacaattc gtgtgtcatg cacttgccgg
     6121 tgctggtgat gcggccgact ccgcagtttt ccttgccacc gcagctggtg gcatcgagcg
     6181 gctcgtcgac ggcgaatatg atgtcggcca ctgtgatatt gcgggcaagg cgcgccagcg
     6241 tgtacccacc gcccggcccg cgcatgctgt cgaccagctc gtggcggcgc aacttgccga
     6301 agagctgttc cagataggac agcgagatat tttcgcgctc actgatggca gccagggtaa
     6361 ccgggccgct ttgatggcgt aacgccagat cgatcatggc tgtgacggcg aaccgccctt
     6421 tggtggtcaa acgcatgaga actccttgcc ttgcggaatt gacgagactg aaacgaagcc
     6481 ttggcagtcg gctgcacgcc tatatcccga ctgtttaact caagtataat caatttccga
     6541 caaatttact tggatataag ggcaagattg cgcaacgcag caaaaaccgc acaaaccatc
     6601 aaccaggaag atcaaccaga gaatgcgggg cggcgaaaac aacgaggccg ctcgtttcga
     6661 gcggcctcgg gaggaaagaa atgcgccgtg ccgtgaggaa tcagtaacgg atcaggcagc
     6721 ctggtattgc gtggcgcgct tgcgcaccag ttcaagcaca ccctggcacg cgtcttcgat
     6781 gtagtcgagc actttgccga acccttccgg ccccccgtaa tagggatcgg gcaccgtggc
     6841 ttcctcgaac tcgttggcaa accgcatcaa gagcatgagc ttgtgctgat agatcttggg
     6901 gcactgctgc tggagcgcag acaggttttc ccaatccatg gccagaatga ggtcgaattc
     6961 gcggaaatcg gccggcacca cttggcgcgc gacacactgc gaaatatcgt aaccgcgctt
     7021 acgcgcggcg gcctgggcgc gggcatcggg cgcttcgcca atatggaagc tgtgcgtgcc
     7081 ggcggaatca accccaacca ccgcggataa ccccgcgtcg tcgaccatgc ggcgaaacac
     7141 gccttcagcg ctgggcgagc ggcaaatgtt gcccatgcaa acaaaaagta ccttagtcat
     7201 cataggtaat agtgtgcggg aaaacccgag acaaggcaag agtttttgca atgtcacaat
     7261 tcgatgcacc cgaaccgcgg tcttatgaaa aacgctgcaa cattaaggga ttagcacgat
     7321 tttcacacac gctcggccat ctggccgagg cgtggcgccg actcttgaag ccctgtcgtt
     7381 cccgcactgc agcaatcaca ccgttcatcg gaattgaaac aaaaccgcac cattgctgac
     7441 gcctcgtggc aatgcaaaac caccagcgcc cgaccacgcg gagtccatcc accgcagcgt
     7501 cgacttggac tgaaatcgtt gccatgccgc aacaccccgc cttcagcatc cgaccgagcc
     7561 gcgcggcggc atcgcgtgga aaaacgcgcc cggttcagct caacagcgcg cgttttttca
     7621 gggcattgag tgcgtcccgg gccgccgccg cctgttcgaa ctcgaggttt cgggcatgat
     7681 ccatcatttg tgtttccagc cggcggatct cccgtgcgag cgccttgtcg tcggccagga
     7741 cttccagcgg cacggcatcc aatgcactat cgggttcggc ctgggccatc acgccgtcta
     7801 tgagttcgcg caccgccttg cgcaccccgc gcgccgtgat cccgtgttcc ttattgaagg
     7861 cgatctgctt gttgcgccgc ctgtcggttt catccagcgc gcggcgcatg gaatcggtga
     7921 tccggtcggc atagaggatg gcacgcccgt tcagattgcg cgcggcgcgg ccgatggtct
     7981 gaatgagact gcgctcggac cgcagaaaac cctctttgtc ggcatccagg atggccacca
     8041 gcgacacttc gggaatgtcg agcccctcgc gcagcaggtt gatacccacc aacacgtcaa
     8101 acacgcccag ccgcaaatca cgcagtatct cgacccgctc gacggtatcg atatccgaat
     8161 gcaggtagcg caccttgacc ccgttctccc ccagataatc ggtgaggtcc tcggccatgc
     8221 gcttggtcag cgtggtgacc agcacgcgct cctggacagt cactcgcttc ttgatctcgc
     8281 ccagcagatc gtccacctgc gtcttggcgg ggcggatatc cacttcgggg tcgaccaacc
     8341 cggtcgggcg caccacctgc tccaccacac tgtcggcatg atttttctca tagtcggatg
     8401 gcgtggccga cacgaacacg cactgccgca tgcgctgctc gaactcctcg agcttgagcg
     8461 gccggttgtc cacggcggag ggcagccgaa agccaaactg caccagggtt tccttgcgcg
     8521 aacgatcgcc acggtacatg ccgcccaact gccccatggt gacgtgactt tcgtcgatga
     8581 acatcaaggc gttcgcgggc aggtagtcga tgagcgtggg gggcggctcg cccggcaagg
     8641 cgcccgacaa atggcgcgaa tagttttcga tgcctttgca gaagcccagc tcctgcagca
     8701 tttccagatc gaaccgggtg cgttgctcga ggcgctgcgc ctcgaccagc ttgccctgcc
     8761 cggtgaacca cgccagccga tcgcgcagct cttccttgat cgtgccgatg gcgcgcaaca
     8821 cggtgtcgcg cggggtcacg taatgggaac tcgggaaaac cgtgaagcgc aacagcttct
     8881 gcttgagttt cccggtaagc ggatcgagca ggtcgatgga ttcgatctcg tcgtcgaaca
     8941 tcgtgatgcg cacggccagt tcggcgtgtt cggccgggaa cacgtcgatg atctcgccgc
     9001 gcgcgcggaa cgtgccgcgc acgaagtcga catcgttgcg ctcatactgc atggccacca
     9061 gccgcgccaa cagttcgcgg cgcgcgatat ggtcgccggt gcgcagggtg agcaccatgg
     9121 cgtgatagtc gccgggattg ccgataccgt agatgcaaga caccgtgccc acgatgatgg
     9181 tgtccggccg ttcgagcagg ctcttcgtgg ccgacagccg catttgttcg atgtgctcgt
     9241 tgatcgaaga gtctttctcg atgaacaggt cgcgcgacgg aacatacgct tccggctggt
     9301 aatagtcgta gtacgagacg aaatactcga cggcgttgtt gggaaagaac tcccgcatct
     9361 cggcatacaa ctgcgcggcc agagtcttgt tgggcgccag caccagcgcc ggacggccgg
     9421 tgcgggcaat gacattggcc atggtgtagg tcttgcccga gcccgtcacg cccagcagcg
     9481 tctggtacat gagcccgtcg tcgacgccct cgaccagcga gtcgatggcc tgcggctgat
     9541 cacccgccgg atgatacggc tggtacagat ggaacgggct gtccggaaac tcgatgaagc
     9601 cgggcccagg cccggtcgac gcggcgggcg aagccacggc agggccgccc gctgcgtccg
     9661 cgggggaatc cgccggtgcg ttgattgccg ctgccatcat cgatccatgt gccggactgc
     9721 tcatattttc tggctgtttg ccgggcatcc tctgaacaag ccaccgcgcc gcgcacatca
     9781 gcggcttggc cagagtatcc ctggacacct gtagggtaaa ccccttatta tcacactatt
     9841 gcgcctttct cttccactct gacttctccc tgccccatga cctccgtttt cgatgcagtc
     9901 gagctcgccc ctcgcgaccc aatttttggt ctgaacgaac aattcaacgc cgacaaccgc
     9961 cccgacaagg tcaacttggg tgtgggggtg tattgcgacg atcatggccg catcccgctg
    10021 ctcgattccg tgcacaaagc ggaagtcgcc cgcgtgcaag ccgcggcggc ccgcggctac
    10081 ctgcccatcg acggcatcgc cgcctacaac aagggcgcgc aggcgttgct gctgggcaag
    10141 gattcacgcg tcctcgccga tggccgcgcc gtcaccatgc aggctctcgg tggcacgggc
    10201 gcgctgaaaa tcggcgccga cttcctgaag acgctgttgc ccaacgcaca agtcgccatc
    10261 agcgaaccca cctgggccaa ccaccgcgcc attttcgaag gggccggttt caaagtcagc
    10321 acctacgcct attacgacgc cgacacccac ggcctgaatt tcgacggtat gctggcctcg
    10381 ctgcgcgccc tgcccgccgg ctccatcgcg gtgctgcatg cctgctgcca caaccccacc
    10441 ggggtggatc ttacacttga ccaatggaag caaatcgccc aggtcgtcaa agacagccga
    10501 ctggtgccgt tcgtcgacat cgcctatcag ggattcggcg acggtctcga cgaagacgcg
    10561 gcggcggtgc gcctgctggc cgacctggac ctcaccatgc tggtcagctc gtcgttctcc
    10621 aagtcgctgt cgctctatgg cgagcgcgtc ggcgcgctca ccgtggtggt cagcggcaaa
    10681 gacgaaggca cccgcgtgct gagccaggtc aagcgcgtga ttcgcgccaa ctactccaac
    10741 ccgcccaccc acggcggcat gctcgtcgcc agtgtgttga acaccccgga actgcgcacg
    10801 ctctgggaaa ccgaactcgc cggcatgcgc aaccgcattc gcgacatgcg caaccagttg
    10861 gtcgaaaagc tggccgccca gggggtgacg cgcgacttca gtttcgtacg cgaacagcgc
    10921 ggcatgtttt cgtattcggg cctcaccagc gcccaggtcg accgcctgcg cactgaatac
    10981 ggcatctacg ccgtgggcag cggccgcatc tgtgtggcgg cgctcaacac ccgcaacatc
    11041 gattacgtcg cgaaggctat tgcccaagtg ctgtaaacag cttgattttc catggggctt
    11101 cgggcagaat tggctgtacg aatatacagc caattctgcc atggccatca aactaaccgc
    11161 acgacagcaa caggttctcg atctcatccg caccgaaatc gcccgcacgg gctttccccc
    11221 cacccgggcg gaaatcgccc gcgcactggg gttcaagtcg gccaacgcgg cggaagacca
    11281 tctcaaggcc ctggcccgca agggtgccat cgaactcacg gccaacgcgt cccgcggcat
    11341 acggcctctt atctgcccgg gcgttccagc caaggccaca tcgcaaatgg ccgaggcctt
    11401 gcaggccggg ctggcgcaat tgctgcttcc gctggttggg cgcgtcgccg ctggcagccc
    11461 tgttctggcg gccgaacacg tcgaacgcga agtcggtgtc gaagcctcgc tgttctccca
    11521 gacgcccgac tatctgctgc gcgttcaggg ctccagcatg cgcgacgccg gcatcctcga
    11581 cggcgatctg cttgcggtca agaaatccgc cgatgcccgc aatggccaga tcgtggtcgc
    11641 gcgcctgggc gacgacgtca ccgtcaagcg cctatcccgt tcaggcagcc acatcacgtt
    11701 gctgcccgaa aacccagact tctctcccat ccacatcacc gccgctgacg atttctccct
    11761 cgaaggcatc gccgtcggcc tcatacgcgc cacgccgctt cattaaggat ccgctgagca
    11821 agttaaaccc caagccgcag atgcatgcgg cacggtgact tgtgcagcgg ttccttaagc
    11881 aaaggcccag gccgcaaagc caaacggcca gccacccaat tctccaacac gagaagagtc
    11941 acagccaaga gtccccacaa cacacgccaa cgcaagggcc aggcggggaa gaaagcggca
    12001 agccgacttt gtcgcaagcc ggccagccaa tcagcgcggg gaggcaaggc gtccgcatgt
    12061 ttgagggcgg cgcttacggc cagccagtgg ctggccgtaa gcccgagttc ggacgccgcc
    12121 ccgcgctgac tggctggccg ggtagcccgg cgcagccggg ccgcagcgac ggaggcctgc
    12181 cgctttcttc cccgcctggc ccgactaaag aacaagcaga caggcccgaa acctgttgaa
    12241 cggttccggg cctgtctgct tgctaccccc tggaacaatt acgcgcccgg ggccatgcga
    12301 tcacactcaa tgcttgaaca actccgacga aggttcaccg ccagacggtt tggcgttcgc
    12361 aactgattcc gccgccaggc gcgccacttc ctcatcggac aatggcgtgg gcatgtgctg
    12421 caaagccact tccagtaccc gatcgatcca gcgcacggga atgatctcca gcgaattctt
    12481 gacgttgtca ggaatctcag ccaggtcctt cacattttcc tcggggatca tgaccacctt
    12541 gataccgccc cgatgcgccg ccagcagctt ctccttcaga ccgccgatgg gcagcacctc
    12601 gccacgcagg gtgatttcac ctgtcatggc gacatcggcc cgaaccggaa tcccggtcag
    12661 cgcggacacc atcgcggtgg tcaggccgat accggccgac ggaccgtcct tgggcgtggc
    12721 gccttctgga aagtgcacgt gcagatcgcg tctctcgaag gccttgtcgg ggatgcccca
    12781 gcgttgcgcg cgggcgcgca ccactgtgcg ggccgcctcg accgattcct tcatgacttc
    12841 acccagcgaa cccgtgcgca gcaccacccc cttgccgggc atgtccgcga cttcgatggt
    12901 cagcaactcg ccgcccacct cggtccaggc caaccctgtc acttggccaa ccttgttttc
    12961 cttcccggca tggccgaaat cgaagcgacg cacgcccagg aaatcattca ggttgtcggc
    13021 gtcgaccttg atggtctcgc gcttggcatc cccccctgcg gcggcggcac ccagcatacg
    13081 cttgaccgtc ttgcggcaga tcttgctgat ttcacgctcc agcgcccgca ccccggcctc
    13141 ccgcgtgtaa tagcgcacga tgtcgcgaat ggctgattca tcgatggaca actcaccctc
    13201 tttcacaccg ttgttcttca tcagcttggg caacagatgg tcgaaggcga tgtgcacttt
    13261 ttcgtcttcg gtgtaacccg acaggcgaat gacttccatg cggtcgagca gcgctggggg
    13321 tatgttcagc gtgttgctgg tcgccacgaa catgacgtcg gacagatcga aatcgacctc
    13381 gacataatga tcctggaagg tatggttctg ttccggatcc agcacctcca gcagtgccga
    13441 cgaggggtcg ccacggaagt ccgcgccaat cttgtcgatc tcatcgagca ggaacaatgg
    13501 attgcgcacc gcgacccgcg tcatgttctg caggatcttg ccaggcatgg acccgatgta
    13561 tgtgcggcga tgaccgcgga tctcggcctc gtcgcggaca ccacccagag ccatgcgaac
    13621 gaacttgcga ttggtggcac gggcaatgga ctggccaagg gaagtcttgc ccacgcccgg
    13681 agggccgacc aggcacagga tgggcgcctt gagcttgtcg acgcgctgct gcaccgccag
    13741 gtattcgacg atacgctctt tgactttttc caagccgtaa tggtcggcgt ccagcacctc
    13801 ttcggcgttg gcaatggagt tgttgacccg gctcttcttg cgccacggca gcgaaatcag
    13861 cgtatcgatg tagttgcgca ccacggtggc ttccgccgac atgggtgaca tcagcttgag
    13921 cttcttgagc tcggcatcgg ctttcttgcg cgcctctttg ggcaaatgcg cggcctcgat
    13981 ctttttttcg agctcctcga tgtcggcgcc ttcctcgcct tcgcccaatt ccttctgaat
    14041 ggccttgacc tgctcgttca gatagtagtc acgctgactc ttttccatct gcttcttgac
    14101 gcggccgcga atgcgtttct cgacctgaag aatgtcgatt tccgcttcga gctgagacag
    14161 cagattctcg agccggcccg aggtgtcgat gatctcgagc attttctgct tctgctcgag
    14221 cttgagcggc aggtgcgccg agatggtgtc ggccagacgc cccgcatcgt cgatgccgct
    14281 gagcgaggtg aggatctcct gtgggatctt cttgttgagc ttgacgtact gttcaaactg
    14341 ggccacgacc gtgcggcgca gcgcctcgga ttccgaattc tggctggcat ccggcgcgat
    14401 gggcaccaca ttggccacga aatgcgtctg cgcgtcgctg acgtgctgga tgctcgcgcg
    14461 ttgcaggccc tcaaccaaca ccttcacggt gccgtccggc aatttcagca tctgcaggat
    14521 gctggccgcg caaccaatgt cgtacaggtc ttccggggtg ggatcgtctt tcccggctga
    14581 tttctgcgcc accagcatga tgttgttgcc ggcctccatg gccagctcga gggccttgat
    14641 ggagcgcgga cggccaacga acaagggaat caccatgtgc ggaaatacga ccacgtcgcg
    14701 caggggcaga agcggcaagt ccatgggttc tgaagagaga atctggctcg cagacatagt
    14761 aacttcctta aaaatcaatg ccttgaaaac aaaaaagggg aagccgtagc ctcccttttc
    14821 agcactactt gaggtgtatg ttgtggcgaa aaagcgtatt tcaagccacc cctgtgcacc
    14881 tatctgggaa taagggcatt accagtactt catcaggccg ccgcatcctt gagtttcttt
    14941 gattgaccgg agcgggagtc ggcggcgcgc gcctcttcct gatctgcgta aatcaatatg
    15001 ggggaaccct tcccggtgac ggcgttctcg tccaccacca cccgttggac attcttctgg
    15061 gagggaagtt cgtacatcgt ggtgagcaac gattgctcga cgatggagcg caggccgcgg
    15121 gcaccggtgc gacgtttgag cgcccgacgc gcgatggcgc tgagcgcttg cggacgcacg
    15181 tcgagttcga cctcttccat ttgaaacagc ttctggaact gtttgatcag tgaattcctg
    15241 ggttcggtca ggatgcgcac cagcgtgtct tcctcgagct cttcgagggt ggccacgacc
    15301 ggaaggcgtc cgaccagttc aggaatgagc ccgaacttga tcaggtcttc gggctcggct
    15361 tcggcgaaca actcaccaat gcctttttcg gtcttggcgc gcaccgatgc cgaaaaaccg
    15421 atacccgatt tctcggtacg gtcacggatg attttttcca atccgtcgaa cgcaccgccg
    15481 caaatgaaca gaatattggt ggtgtcgacc tggacgaaat cctgattggg atgcttgcgc
    15541 ccgccttgcg gcggcaccga cgccaccgtg ccttctatga gcttgagcaa cgcctgctgc
    15601 acgccctcgc ccgacacgtc gcgcgtgatg gacgggttgt cggccttgcg tgagatcttg
    15661 tcgatttcgt cgatgtagat gatggcgcgt tgcgcctttt cgacatcgta gttgcagttc
    15721 tgcagcagct tctggatgat gttctcgacg tcttccccca catagcccgc ttcggtgagg
    15781 gtggtggcat cggccatcac gaacggcacg ttctgcatac gcgcgagcgt ttgcgccagc
    15841 agtgtcttgc ccgagcccgt gggcccgacc agcaggatgt tgctcttgga gagctcgaca
    15901 tcgtcaccct tgacttcggt gtagcgaacg cgcttgtagt ggttgtagac cgccaccgcc
    15961 aggctgcgct tgggggcctc ttggccgatc acgtagccat cgaggaacgt cttgatctcg
    16021 acgggcgtgg ggagttcgtt gcgaatggca tcgcgcgccg catcgcgggt ttcctcgagg
    16081 atgatgtcat tgcacaggtc gatgcactcg tcgcagatga acaccgacgg ccccgcgatg
    16141 agcttgcgca cctcgtgctg actcttgttg cagaacgagc aatgcagcac cttgtcgtcc
    16201 gccgaacctt ttttttcagc catggatata tttatcgctt caaacgaacg agcgcacgcg
    16261 gctcgttcag gagggaatca cccacgcaca caatgaccgt tcaccccaca ggggaaccgc
    16321 ctacttcttg gtgtcggccc gggaaaccag cacatcgtct atcagaccat acgataccgc
    16381 atccgcggcc gacatgtagt tgtcgcgttc ggtgtcttcg gcgatgcgct cgagaggctg
    16441 tccggtacgc tcggccagga tgctgttcaa gcgctcgcgc agactgagaa tctcgcgtgc
    16501 ctggatctgg atgtcagagg cttggccctg ggcgccgccc gaaggttggt gaatcatgat
    16561 gcgcgaattc ggcagcgaat agcgcttgcc cttggcgccc gccgccagca ggaacgcgcc
    16621 catgctggcc gcgaaacccg tgcacagcgt ggacacgtcg ggcttgacga actgcatggt
    16681 gtcgaaaatg gccatgcccg aatacaccac tccgccaggc gagttgatgt agagggaaat
    16741 gtccttgtcg ggattctctg attccaggaa caacaactgc gcgacgatga ggttggcgga
    16801 ctggtcttcg accggcccga ccagaaacac cacgcgctcg cgcagcaggc gggaatagat
    16861 gtcgtacgag cgctcgccgc gcccggactg ttctatcacg ataggcacat agcccagacc
    16921 gctgggccgt accgagtccc cgccatgcat tgatgcgtag aaatcagtga aatgcgacat
    16981 atgaggattc cttaagcggt tcccatgagt tcgtcgaagg cgacctgttc gtcggtgacc
    17041 tgggccttgg acagcacata gtcgacgacg ttgtcttcaa gaacgatggc ctcgatctcg
    17101 gcgcggcgct gccggtcgga cagataatac gccacgacct gggcaggctg ctcgtagctc
    17161 ttggcgaagt cttcgatgcg ggtgcgaacc tgctcgggct tggctttcag atcggccttg
    17221 tcgaccagct cggaaaccag cagacccagc ttcacgcggc gctcggattc ggccttgaac
    17281 atgtcttcgg gaatgggcac tgattcggca ttgggcacgc cgcgttgctt gaggtcttcg
    17341 cgggcagcgg cgatgcggct ttcggtatcg ctttgaacca gagccttggg cacatcgaac
    17401 gagcacgccc caaccagcgc gtccatgacg ctgcccttgg tgcgggcctg cgcccgggcc
    17461 ttgacctcgc ggtcgacatt gccacggacc tcggccaaca gtttgtcgac gtcgccctcg
    17521 gcctggccca gcgacttggc gaattcggca tcgactgcgg gcagcacggc ctcggccact
    17581 tcggtgaccg tgatcgcgaa ctcggccttc ttgccggcca cttccttgcc accataatcg
    17641 gccggaaatt caagcgggaa ggttttgttt tcacccgcct tcatgccttg cacggcggct
    17701 tcgaattccg gcagcatgcg gccctggccc agcacgaacg ggaatttctc ggccttgccg
    17761 ccatcgaacg gcaccccgtc gatggtgccg gcgaaatcca gcgtgacgcg gtcgccatcc
    17821 tgggcggcac ggccttcacg cgcttcgtag ttggcgcgct gcttgcgcag gatgtcgatc
    17881 gtgtgctgca cgtcggcgtc ggacacttcg acattggcgc gcgtgacctt cagcgcggcg
    17941 atatcaggcg cggtgatttc cgggtagacc tcgaacgtgg ccgagaacgc catgatgcct
    18001 tcctcgaccc cttccgtctt gggttcgaga ttgggcgtgc cggcgacgcg cagattcgcg
    18061 tcctggatgg ccttgtcgag cgcacggccg acttcggtat tgatgacgtc ataccggatg
    18121 ccggggccat ggctgcgctc gatcatggcg agcggcgcct tgcctggacg gaagccctgc
    18181 accttggcgg tacgcgcgac acgcttgagc tgcgcctgga cttgcttttc aacttcggcc
    18241 aggggaacga ccagatcaac tcggcgctcc aggccggaca gagtttcaac cacgggctgc
    18301 atcggataag gacctatatg tgaacaatcg ggacagtcga acggggtatt ttaccggaaa
    18361 ttgaaaacac caagcctgcc ccgtcaagcg ccgggacggg aaagcggccg atcgggagaa
    18421 cggcccatac tcttaaaatg gttcctgtcg ccaaccattg gcccaaaccg gaaccaacat
    18481 gcccaaaccc ctggacggag tacgtgtgct cgaactcgga caactcatcg ccggaccgtt
    18541 cgccgccaag atgctggggg agttcggcgc cgaggtcatc aagatcgaac cgcccgaaac
    18601 cggtgacccg cttcggaaat ggcgcctgtt gcacaacggc acgtcggtat ggtgggcggt
    18661 gcaatcgcga aacaaaaaat ccgtcaccct cgatctgcgt gagcccgaag cccaggatgc
    18721 ggtgcgcaag ctggccgcca ccgccgacgt ggtgatcgag aacttccggc ctggaacgct
    18781 tgagggctgg gggctgggtt gggatgcctt gcacgcactc aatccccggc tcgtcatgct
    18841 gcgggtttcg ggatatgggc agaccggccc ctatcgtgat cgacccggct tcggcgtcgt
    18901 gggtgaagcc atgggcggtc tgcgccacct cagtggcgaa cccggccgtc ctcctgtgcg
    18961 cgtgggggtg tccatcggcg attcgctgtc ggcgctgcac ggcgtgatag gcgtgctgtt
    19021 ggcccttcgc cagcgcgacc agaacggcgg cgacggccag tgcgtggatg tggcgctgta
    19081 tgaatccgtc ttcaacatga tggaaagcct gttgccggag tacgccgtct tcaatgaggt
    19141 gcgccaaccc gccggcagca gcctgcccgg catcacgccc agcaacgcgt acctgtgcgc
    19201 cgatggcaag tacgcactca tcgccggcaa cggcgacagc atcttcaaac ggctcatggt
    19261 ggccataggc cggccggaag tggccgagaa ccccgcctat gcccacaacg acggacgcgc
    19321 acgcgacgcc gtcacgatcg acggcatcat tggcgactgg gcgcgcggcc acgatctgga
    19381 gcacgtgctc cacgtcctgc acgaagcccg catccccgcc ggcaaggtct acgacatcgc
    19441 cgacatctgt gccgatccac attatcaggc gcgcggcatg atcctcgaca gcacactgga
    19501 tgacggcacc cccgtcaagc tgccgggcat cgtccccaag ctggacgcga ctcccgggga
    19561 cgtgcggcgc ggcgcgccca cactgggtca ggacaccgat caagtgctgg gtgaactggg
    19621 gatagacgcc aaaacgcgcc aaggctggcg ggaacgcggc atcatctgat tcaaatgtgc
    19681 ctcgacgatc catcgataca caagcaaacc acttcctgag gcaccacgcc gacctcgaaa
    19741 cctagggtta atactagtat tcggattgat ctagtcatgt aaacatatat atgaataggg
    19801 agactgacat gttgatcgat aatcgccttc ctcgctatca acgtctgcgc gacgaattga
    19861 tcacgctgat cacagcgggt cagtggcgac ccggggaaac cattcccact gaacaatcac
    19921 tggctcagac ccacaacgtc gcaatcggca ctgtccgcaa ggccgtcgac ctgcttgtgg
    19981 cggaaggtct gttggagcgc tttcaaggcc gggggacatt cgttcgacgc gccagtttcg
    20041 acaattcgct gttccgcttt ttccggttcc agactcgaca gggcgagcga cgcattccgg
    20101 aaagccgcat ccttcgacgc gaggtggtcg tggcgccctc ggcggtggcc accgccttgc
    20161 aggtggcgcc cggcaccgag gttatccaga tgtcccggct ccgtctagcc aatgacgtgc
    20221 cgctactggc cgaagaaatc tggttgccgc acgaccgctt cacggcattt ctccaattgg
    20281 atctgaccag tatcggcaat ctgctctatc ccgtctacga ggcgcaatgc aatcaaatcg
    20341 ttgcctcggc gacagaaacg cttaccgtcg aggccattaa tccgatgtat gcgcgccttc
    20401 tcaaaatcgc cccgggcact cctggcgtgg tgatcgagcg actggcttac ggctacgacc
    20461 gccagcccct ggaatggcgg cgctcacgag ggccggccag tgaattcatt tatcaggtcg
    20521 aaatccgctg acgaccggct catatcagcc ggtgcgggcg atcccgcccg ccattcgccc
    20581 cccctctctg gagacaaaca tgttcagttg gttcaaagaa gtatccagca acgaacgccg
    20641 gacgttctgg gcctgcttcg ggggctgggc cctcgacgcg ctcgatgtcc agatgttcag
    20701 tcttgtcatc cccgccatca tcgctgaatg gtcaatcagc cgaaccgacg ctggcgtggt
    20761 aagtggcgcc actttggtgg cctcggcgct gggaggctgg gtcgctggcg ccctgtcgga
    20821 ccgctacggc cgcgtacgca tcctgcaatg gaccgtggct ttcttttccg tattcacatt
    20881 cctgtccgct ttcgcgcaaa gctatcccca actactcatc ctgaagtcgc tgcaaggatt
    20941 cggtttcggc ggggagtggg ccgcgggagc ggtattgatg gcagagacca tccgcaccca
    21001 acatcgcgga aaggcgatgg gcagcgtgca aagcgcatgg gcggtcgggt gggccgccgc
    21061 agtgttgctt tatgccctga tgttttccct gtttccggga gacatggcat ggcggctcat
    21121 gttcgcgctg ggcatcctcc ccggactcct ggttttgtac attcgcagag gtgtgcaaga
    21181 gccca
//
LOCUS       DASTNK010000139        10016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1037537, whole genome shotgun sequence.
ACCESSION   DASTNK010000139 DASTNK010000000
VERSION     DASTNK010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10016
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037537"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VFR20_10355"
     CDS             complement(<1..193)
                     /locus_tag="VFR20_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230963.1"
                     /translation="MSLSDSIDYAAAPQHAITTASTHRTMTPTILRQYQTIADISSKM
                     LDLARTDKWDQVVELGHAYH"
     gene            complement(190..621)
                     /gene="fliS"
                     /locus_tag="VFR20_10360"
     CDS             complement(190..621)
                     /gene="fliS"
                     /locus_tag="VFR20_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HEU0230964.1"
                     /translation="MNHVQTRIRPGARSAQAYADIGLETRVSSATPEQLITLLFDGAL
                     AAIAKARLYLHNGDTSGRGKALSNAIDIVESGLKASIDTDAGGDLANNLVAVYDTVVR
                     HLLLANMNADAANLDVAERLLADIGGAWRDIAGTRSAEAQS"
     gene            complement(636..2009)
                     /gene="fliD"
                     /locus_tag="VFR20_10365"
     CDS             complement(636..2009)
                     /gene="fliD"
                     /locus_tag="VFR20_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HEU0230965.1"
                     /translation="MSSTITSLGVGSGLPLDTLMTQLQNSENTALQAIQTKQVAAQTT
                     LSAYGKIQSAVSALQTAAQTLTNTSTYGALKTSFSGSALTASTSTGAIAGQYSITVDQ
                     LATAQTLVTSGQASQTQANGTGGAITFKLADGTTTTLDMTGKDTSLNGLISAINGNPD
                     LGVSATLINDGSSTAYHLLITSNTTGTSAAVTNISSTNASLQGILGFTQGTPSANITE
                     TAATNATLHINGIQITSQSNTVENAIQGVALTLNQVNTTPSTLTIVHDDSATTQAVQG
                     FVSAYNSLQSTIQSLTAYDTSTNTGSTLTGDSLARSVQNQMSNALNTVVSGGMINNLY
                     QLGINISPTDGTLTVDSTKLSQAVSSNLGDVQNLLGGTGGVGTAVNSLATNMLGTGGL
                     FKSASDSLNQTLTDLQTQYSQTSDRISTVMDTYRKQFTALDSTVAQMNSLSSYLTQQL
                     SHLGTSS"
     gene            complement(2131..2517)
                     /locus_tag="VFR20_10370"
     CDS             complement(2131..2517)
                     /locus_tag="VFR20_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HEU0230966.1"
                     /translation="MAIPPVSGMPGFPAVSTTGGTDHAPTNRPPEAVGPDSAMPAGTV
                     GGHGGASADATPTPLDKAITLANSSLEAWSTGMRFDIDPTLGRVVISITDSRTGKVLR
                     TVPTDAVLRVAKMILQMREKPIDTKA"
     gene            2782..3543
                     /locus_tag="VFR20_10375"
     CDS             2782..3543
                     /locus_tag="VFR20_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HEU0230967.1"
                     /translation="MPASEDQMVGQYAPLVRRLALQLAAKLPASVELDDLMQAGMMGL
                     LDAVRRFRDTAEAQFETYAVTRIRGAMLDELRAQDWLPRSVRGKARHIEQAVRHLHQT
                     LMRPPGEQEIAEHMGLPLADYQSLLDEVRGVQIVHYEDLCRDPGSSSDASDRLDVASE
                     SRDTWSNPLDRLLTQGLREALIAAIEDLPDRERLLVSLLFEQDLNQKEISLVMGVTEG
                     RVSQLRSQAVTRIRAALAGNVWYTDPGEVDLHALL"
     gene            3712..3939
                     /locus_tag="VFR20_10380"
     CDS             3712..3939
                     /locus_tag="VFR20_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230968.1"
                     /translation="MDMVPVYRPKSDSEALVVVALMQAHGIVHFMRGGAFSSMYPGPI
                     STSLNAQMLMVESSQADSARELLSGFLDDDT"
     gene            complement(3956..4384)
                     /locus_tag="VFR20_10385"
     CDS             complement(3956..4384)
                     /locus_tag="VFR20_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0230969.1"
                     /translation="MSRPIRSQLWLQIAVVYFVLAVALGLAMGAAGDFTLMPVHVQLN
                     LLGWIAMSLIGLATARFPQLGYGRLAVAQFWLYNLGVPVMLLALAAGIHGRTGAEHVA
                     TAASAIVALSLGMLACQVGLCMRQSRLAHVRHGWHRACGP"
     gene            complement(4535..6001)
                     /locus_tag="VFR20_10390"
     CDS             complement(4535..6001)
                     /locus_tag="VFR20_10390"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEU0230970.1"
                     /translation="MARIGIAGFLHETNTFAPGLTSYDQFVQADAWPGLITGRHLLEE
                     TRGMNLAVSGFIAALENTPHKAVPLLWCSANPSGIVEQAAFDAVVGQIEYLLDAAAPL
                     DALFLDLHGAMVTQVHDDAEGALLARLRARLGPDLPIVAALDFHANVSQRMAENASGL
                     IAYRTYPHVDMAETGLRAARFLFRLLNEATPARAYRKLPYLIPMPWQSTLAEPMRSLM
                     DTAIHACTGTRVWEAQIVPGFPLADVIDSGPGILVYAQDPGTANTVADALCADMETAR
                     DRFSGKLYPVEEAVSHALSMPPGKRLILADTQDNPGGGGPGDTTDLLRALRDQGAQGV
                     CAGVICDPAFAAAAHGAGPGKFLDASLGGQSGIGGPGLQGKYEVMRIGDGRFTGTGPF
                     YLGCHMDLGPMARVRLNGIDIIVSSRKQQAADQAMFRHVGAEPAAYRILVLKSSVHFR
                     ADFAALANEILVVEAPGPNIANLHTLRYRHLRPGTALL"
     gene            complement(6001..7164)
                     /locus_tag="VFR20_10395"
     CDS             complement(6001..7164)
                     /locus_tag="VFR20_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU0230971.1"
                     /translation="MTRLSVNWNYPTAIKAGPGRIRELPQHCRQLGMKRPLLVTDPGL
                     ATLPMVAGVVANCREAGLECAVFSHIKGNPTGTNVQDGVAAYKAGGHDSVIAFGGGSG
                     LDAAKAIALVAGQSRPLWDFEDIGDNYLRAEVNAIAPILAVPTTAGTGSEVGRASVIT
                     DEAEHVKRIIFHPRMLPGLVILDAELTTGLPARLTAATGMDALSHNLEALCSPVHHPM
                     ATGIAVEGIRLVRQHLPGAVRDGGDIDARHHMLVASSMGATAFQKGLGAMHALAHPLG
                     ALYDAHHGMLNAILMPYVLLANRDLVNEKMTRTARYLGLADASFQGFLDWVLRLRDDV
                     GIPHTLRDIGIPDDRADAIGHMAVADASASTNPIPFTPAQYTDILHNALDGRL"
     gene            complement(7174..8562)
                     /locus_tag="VFR20_10400"
     CDS             complement(7174..8562)
                     /locus_tag="VFR20_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013397036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0230972.1"
                     /translation="MSTELVTISPIDGRELLRRPYTSEADIARALERAKAARRDWGAR
                     PLAERCAAVSAAVDRFVAGKAEAAEAITRQMGRPLRYTPGEVNGFEERARHMIAAAPE
                     ALAPIVLPRKDNFTRYIDREPVGTVLTIAPWNYPLLTAVNSIVPALVAGNTVLLKHSD
                     QTPLCAELLVRAFTESGLPDGVFQYVHATHDTVGRLMRAPEIGYVSFTGSVFGGQAVE
                     KALAGRFIGAGLELGGSDPAYVRTDADLEQAVATLVDGAYFNSGQSCCGIQRIYVARQ
                     RYQEFVDRAAALTAQYVLGDPMSPDTTLGPVVRLAAAREIRGVVRDAIRQGARNLVNP
                     APFGAARDDNAYVAPALLVNVDHTMAVMNEECFGPVAGIMPVDSDEQAIGLMNDSHYG
                     LTACVFTQDADAAVRIGRQVETGTFFMNRCDYLDPGLAWTGVKNTGHGISLSTLGYHA
                     LTQAKSYHLRTL"
     gene            complement(8670..>10016)
                     /locus_tag="VFR20_10405"
     CDS             complement(8670..>10016)
                     /locus_tag="VFR20_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU0230973.1"
                     /translation="RLVKESGLTHIKLGMSDIDGVMRGKYVRQDKFLGALKSGLAFCD
                     VVMGWDSNDAMYDNVQFTGWHTAYPDAQVRIDPVSCRRLPLEKNADGHDMLFFLAEFD
                     GRAAEVCPRRLLKRVIDKARDMGLDAFSALEYEFFMFNETPKSVREKNYRNLETWTPG
                     NFGYSVLRSTVNSDFYAELIDLTEKMDMSIEGLHTETGPGVLEAAIAYDEIAKAADKG
                     FLFKTFTKALAEQHSLMATFMAKWSHDLPGQSGHIHLSLRDRQSGGNAFFDESQPYNM
                     STVHRQFVAGLQRYLPEFMALYAQTINSYSRLVPGYWAPLNATWAIENRTTALRVIPG
                     SEKSQRVEVRIGSADANPYLALAAALGSGLAGIEQKLEPGPMVTGNAYAQTFPEHLRL
                     PATLWDAAQRLRQSETARALFGAPFVEHFAATREWEEREFRRHVTDWELKRYFEII"
BASE COUNT         1661 a         2928 c         3460 g         1967 t
ORIGIN      
        1 tatggtaggc atggcccagc tcaacgacct ggtcccattt gtcggtgcgg gccagatcca
       61 gcatcttgct gctgatgtcg gcgatggtct ggtactggcg caggatcgtg ggggtcatgg
      121 tccggtgtgt ggaggcggtg gtgatggcgt gctgtggcgc ggctgcgtaa tcaatggaat
      181 cggaaaggct catgattgtg cctctgctga tcgtgtgcca gcgatgtcgc gccaggcgcc
      241 accaatgtcg gcaagcaggc gttcggcgac atcgaggttg gcggcgtcgg cgttcatgtt
      301 ggcgagcagc agatgacgaa caaccgtgtc atagaccgcg acgaggttgt tggcgagatc
      361 gccgccggcg tcagtgtcga tgctggcctt gagccccgat tcgacaatat cgatggcatt
      421 ggacagggcc ttcccgcggc cactggtgtc gccattgtgc aaatagagcc tggccttggc
      481 gatggcggcc aacgcgccgt cgaacaacag agtgatcagt tgttcgggtg ttgcgctcga
      541 tacgcgggtt tccaggccga tgtcggcgta ggcctgggct gagcgggcac caggccggat
      601 gcgggtttgg acgtggttca tgattggggc cgggttcagg aggaagtgcc cagatgggag
      661 agttgttggg tcaggtacga actgagggaa ttcatctggg ccacagtcga gtcgagcgcg
      721 gtgaattgct tgcgatacgt gtccattacc gtgctgatgc ggtccgaggt ctggctgtat
      781 tgtgtctgca ggtcggtgag tgtctgattc aggctatcgg aggcggactt gaatagcccg
      841 ccggtgccca acatgttcgt ggccagggaa ttcacggcgg tgccgacgcc accagtgccc
      901 cccaggagat tctgaacgtc gccgagattg ctggacacgg cctgggacag cttggtcgaa
      961 tcgaccgtca gggtaccgtc ggtgggactg atgttgatgc ccagttggta caggttgttg
     1021 atcattccac ccgacaccac ggtgttcagg gcattgctca tctggttctg tacgctacgc
     1081 gccagggagt ccccggtcag ggtgctgccg gtgttggtcg aggtgtcgta cgcggtgagt
     1141 gattgaatgg tggactgcag gctgttgtag gccgagacga aaccctggac ggcctgtgtc
     1201 gtcgcggagt cgtcgtgcac gatggtcagt gtcgagggcg tggtgttgac ctggttcagg
     1261 gtcagggcga caccctgaat ggcgttttcc accgtgttgc tttggctagt gatttgtatg
     1321 ccgttgatgt gtagtgtggc gttggtggcc gcggtttctg tgatgttggc cgacggagtg
     1381 ccctgggtga accccagtat gccctgcaac gacgcgttgg tgctgctgat gttggtgacc
     1441 gccgcgctgg tgccggtcgt gttgctggtg atcagcaggt gataggccgt ggatgagcca
     1501 tcgttgatca acgtggccga cacccccaga tcggggttgc cattgatggc gctgatcagg
     1561 ccgttgagcg aagtgtcctt gcccgtcatg tcgagcgtgg tggtggtgcc gtcggccagc
     1621 ttgaacgtga tcgcgccgcc ggtgccattg gcctgggtct ggctggcctg gcccgaggtg
     1681 acgagtgttt gcgcggtggc aagctggtcc accgtgatgc tgtattggcc cgcgatggcg
     1741 ccagtgctgg tgcttgccgt cagcgcactg ccgctgaaac tggttttgag ggcgccatag
     1801 gtactggtgt tggtcagggt ttgcgccgcg gtctgcaggg cgctgaccgc gctctggatc
     1861 tttccatagg ccgatagcgt ggtctgcgcc gcgacctgct tggtctggat ggcctgcagc
     1921 gccgtgtttt cactgttctg cagctgggtc attaaagtgt ccagaggcag accggagccg
     1981 acgccgagcg aagtgatcgt ggatgacata tgtggtttct ccctacgagg gtattgtcag
     2041 atgaccgggt ctcgggcgat cccgacgtca gtagtcataa atcttggcat tttgccgctt
     2101 tggtgcttgt gccagtggcg cattggcgtg tcaggccttg gtgtctatgg gtttttcccg
     2161 catctggagg atcattttcg cgacacgaag gacggcgtcg gtggggacgg ttcgcagtac
     2221 cttgccggtg cggctgtcgg tgatggaaat gacgacccgc cccaaggtgg gatcgatatc
     2281 gaatcgcatc ccggtggacc atgcctccag gctgctgttg gcaagggtga tggctttgtc
     2341 gagcggcgtc ggggtggcat cggcagacgc cccgccgtgg ccgcccaccg tgccggccgg
     2401 catggcggag tcaggaccaa cggcttccgg cggtcggttg gtgggggcgt ggtcggttcc
     2461 cccggtggtg gaaaccgccg ggaaccccgg catgcccgaa acgggaggga ttgccatgat
     2521 gcttctcctg ggatgcgcgg gacgcccccg cctggctggg ttcgtcatgg acgtggtcca
     2581 cccggcgtga tgcctcgcgt gacgccttct gtttgtcggc tttacggtgc gggagcgttg
     2641 aaactttagg gcaccgcgct caaccaggca caggaagcgc aaaggcgcga acaggcaggc
     2701 gaattcggcg ctgattccgc caatacaccg gactcgcggc gggtgacaat acgttcactc
     2761 gtctagctcc atttcgtgcc catgcctgct tcagaagacc aaatggttgg ccaatatgcg
     2821 ccgctggtgc ggcggctggc cttgcagctc gcggccaagc tgcccgcgag tgtcgagctc
     2881 gacgatctga tgcaggccgg gatgatgggg ttgctggatg ccgtgcggcg ctttcgcgat
     2941 acggccgaag cgcagttcga gacctatgcc gttacgcgca tccgcggcgc gatgctcgac
     3001 gagttgcgcg cccaggattg gttgccgcgc agcgtgcgcg gcaaagcccg gcacatcgaa
     3061 caagctgttc gtcacctcca tcagaccctg atgcgcccgc ccggcgagca ggaaatcgcc
     3121 gaacacatgg ggctgccgct ggccgattac cagtccttgc tcgatgaggt gcggggcgtg
     3181 cagatcgtcc attacgagga tttgtgccgt gacccgggct cttcctccga tgcgtcggat
     3241 cgcctcgacg tcgcgtccga gtcccgtgac acgtggtcca atccgcttga tcggctcctg
     3301 acccaaggtt tgagggaagc cctgatcgcg gcgatcgaag atctgcccga tcgtgaacgg
     3361 ctgctcgtgt ccctgctctt cgagcaggat ctcaaccaga aggaaatcag cctggtcatg
     3421 ggggtgaccg agggtcgcgt gtcccagttg cgctcgcagg cggtgacgcg gatccgcgcc
     3481 gcgctggccg gcaatgtctg gtacaccgat ccaggcgaag tcgatcttca tgccctgttg
     3541 tagggccgga cggtttcatc ggcgctttgc cgcgattccg ccgcgttctt gatgccgcct
     3601 cccgcgtcac ccgtgacgcc ccggttcgtt tgcgtgataa tggctccgtg accggcccct
     3661 ggcccgaatg tgggggtgcc ggcctggtgt caggcgaaca gggggaagcg catggacatg
     3721 gtacccgtct accgtccgaa gtcggattcc gaggcgctgg tggtggtggc gctcatgcag
     3781 gcgcacggta tcgtccattt catgcgcggt ggtgcgttca gcagcatgta cccgggcccc
     3841 atttccacca gcctgaatgc gcaaatgctg atggtggaat ccagtcaggc cgattcggcg
     3901 cgtgaactgc tttcgggatt cctggatgac gatacttgaa tcgagaccct gttcatcatg
     3961 gcccacatgc gcgatgccag ccgtggcgca catgggccag ccgggattgc ctcatgcaca
     4021 ggcccacttg gcatgccaac attcccaggc tcaatgccac tatcgctgaa gcggcggtgg
     4081 ccacgtgttc ggcgcccgta cggccgtgga tgccggcggc gagcgccaac agcatgaccg
     4141 gcacgcccag gttatacagc cagaattgcg cgacagcgag gcggccatac cccaattgcg
     4201 ggaaccgcgc cgtggccaat ccgatcaggc tcattgcgat ccagcccagc agattgagct
     4261 gcacatgcac cggcatgagc gtgaaatcgc cggccgctcc cattgccaag cccaacgcca
     4321 cggcaaggac gaaatacacc acagcgatct gcagccagag ctggcttcgg atgggacggc
     4381 tcatgatgat cccgatctta gtcctccggt ggcgcggcat caatcggcgg attggcgggt
     4441 gttccgtgga tccaacactt ggcgcacagg caagccgtgt gcgggtctgc cagccccggg
     4501 cgtctgagcc tggcggcggc cttggagtcg gggatcagag cagggcggtg ccggggcgca
     4561 gatgcctgta tcgcagggta tgcaggttgg cgatgtttgg gcccggcgct tcaacgacca
     4621 ggatttcatt ggccagcgcg gcgaagtccg cccgaaaatg aacagagctc ttgagcacca
     4681 ggatgcggta cgccgccggc tccgcgccga cgtggcggaa catggcctgg tcggccgctt
     4741 gctgtttgcg gctggatacg atgatgtcga tgccgttcag ccgtacccgc gccatgggcc
     4801 cgagatccat gtgacacccc agatagaacg gcccggttcc ggtgaatcgg ccatcgccga
     4861 tccgcatgac ctcatacttc ccttgcaggc cggggccgcc aatgccgctc tgtccaccaa
     4921 gggacgcgtc caggaacttg cctgggcccg cgccatgggc cgccgcggcg aaagccgggt
     4981 cacagatcac accggcacaa acgccttgcg cgccctggtc gcgcagcgcc cgcagcagat
     5041 cggtcgtgtc gcccgggccg cctccacccg ggttgtcctg cgtgtcggcg aggatgagcc
     5101 gcttacccgg cggcattgac aaggcatggc tgacggcctc ttccaccgga tagagtttgc
     5161 cgctgaaccg atcgcgggcg gtttccatgt cggcgcacaa ggcatcggcg acggtgttgg
     5221 ccgttcccgg atcctgcgcg tacaccagga tcccggggcc gctgtcgatg acgtcggcca
     5281 gagggaagcc gggcacgatc tgggcttccc agacgcgggt accggtgcag gcgtgaatcg
     5341 ccgtgtccat caacgagcgc atgggttcgg ccagagtgct ttgccagggc atgggtatca
     5401 ggtacggcag cttgcggtag gcgcgggccg gggtggcctc gttgagcaga cgaaagagaa
     5461 accgcgcggc gcgcagcccg gtttcggcca tgtccacatg cgggtaggtg cggtacgcga
     5521 tcaggccgct ggcgttctcg gccatgcgct ggctgacgtt ggcgtggaaa tcgagggcgg
     5581 cgacgatggg caggtccggt cccaggcgcg cgcgcaggcg tgcaagcaat gccccttcgg
     5641 catcatcgtg gacctgagtg accatcgcgc cgtgcaggtc caggaacaac gcgtcgagcg
     5701 gtgccgccgc gtcgagaagg tattcgattt gaccgacaac cgcgtcaaac gcggcctgtt
     5761 ccacgatgcc cgacgggttg gccgagcacc agagcagtgg cacggctttg tggggagtgt
     5821 tctccagcgc cgcgatgaac ccgctgacgg ccaggttcat gccacgggtt tcttccagaa
     5881 ggtggcggcc ggtgatgaga cccggccagg catcggcctg aacgaactgg tcgtatgagg
     5941 tcagtcccgg cgcgaacgtg ttggtttcgt gcaggaagcc cgcgatgccg atccgtgcca
     6001 tcacaacctg ccatccagcg cgttgtggag aatgtctgtg tattgagcgg gcgtgaacgg
     6061 aataggattg gtcgatgccg aggcatcggc cacggccatg tggccaatgg cgtccgcgcg
     6121 atcatccggg ataccgatgt cgcgcagcgt gtgcgggata ccgacgtcat cgcgcaggcg
     6181 cagtacccaa tccaggaaac cttggaatga cgcgtcggcc aacccgagat aacgggcggt
     6241 gcgtgtcatt ttttcgttca ccaggtcgcg gttcgccagc aggacgtagg gcatgaggat
     6301 ggcattgagc atgccgtggt gcgcgtcata cagggcgccc aggggatggg ccagcgcgtg
     6361 catggcaccg agccccttct ggaaggcggt ggcgcccatg ctcgatgcca ccagcatgtg
     6421 gtgtcgtgca tcgatatcgc caccgtcgcg cacggcgccc ggcaggtgtt gtcgcaccag
     6481 tcggatgccc tcgacggcga tgccggtggc catggggtgg tgcactggag aacatagcgc
     6541 ttccaggttg tgggacagcg cgtccatgcc cgtggcggct gtcaggcggg ccggcaggcc
     6601 ggtggtgagt tcggcgtcga gaatcaccag gccgggcagc atgcgtgggt ggaagatgat
     6661 gcgtttgaca tgctcggctt catcggtgat gaccgaggcg cgacccactt cggagccggt
     6721 gccggccgtg gtgggcaccg cgagaatggg agcgatggca ttcacctcgg cacgcagata
     6781 gttgtcgccg atgtcttcga aatcccagag cgggcgggat tggccggcca ccagggcgat
     6841 ggccttggcg gcgtcgagcc ccgagccgcc accgaaggcg atgacactgt cgtgtccccc
     6901 cgccttgtat gccgcgactc cgtcctggac gttggtgcct gtcggattgc cctttatgtg
     6961 ggagaacacg gcgcattcca gtcccgcctc acgacagttg gcgacaacgc ccgccaccat
     7021 ggggagcgtg gccaggcccg ggtcggtgac gagcaatggg cgtttcatgc ccagttgcct
     7081 gcagtgctgc ggcagttcgc gtatgcggcc gggccccgcc ttgatggcgg tggggtaatt
     7141 ccagttgaca gaaagtcgcg tcatgatgaa tgcctacaga gtacgcaggt gataggattt
     7201 cgcctgggtc agcgcgtggt agcccagggt ggaaagcgag atgccatggc cggtgttttt
     7261 gacgccggtc cacgccaggc cagggtctag atagtcgcag cggttcatga agaatgtgcc
     7321 ggtttcgacc tgtcggccaa tgcgcacggc ggcgtcggca tcctgtgtga acacgcaggc
     7381 ggtcaaccca taatggctgt cgttcatgag gccgatcgcc tgttcgtcgc tgtcgaccgg
     7441 catgatgccg gccacggggc cgaagcattc ctcgttcatc accgccatcg tatggtcgac
     7501 attgaccagc aacgcggggg cgacataggc gttgtcatcg cgcgccgcgc cgaaaggcgc
     7561 gggattcacc agattgcgcg ctccctggcg aatcgcatcc cgcaccacgc cgcggatctc
     7621 gcgcgcggcc gcgagacgca ccaccggccc cagggtggtg tcgggtgaca tcgggtcgcc
     7681 gagcacgtac tgcgcggtca gtgcggcggc gcgatccacg aactcctggt agcgttggcg
     7741 ggccacataa atacgctgga tgccgcagca agactgtcct gagttgaagt aggcgccgtc
     7801 gaccagggtg gccactgcct gttccagatc ggcatcggtg cgcacatagg ctggatcgct
     7861 tccgccgagt tccagtccgg cgccgatgaa ccgccccgcc agcgcctttt ccaccgcttg
     7921 tccgccgaac acggaccccg tgaacgacac atagccgatt tcgggcgccc gcatcaggcg
     7981 ccccacagtg tcgtgggtgg cgtgcacgta ctggaacacc ccgtcgggca gccccgattc
     8041 cgtgaaggcc cgaaccagca gttcggcgca cagtggcgtc tgatcggaat gtttgagcag
     8101 caccgtgttg cccgccacca gcgctggaac gatgctgttg acggcggtta gcaacggata
     8161 gttccagggg gcgatggtca gcaccgtgcc gacgggctcg cggtcgatgt agcgggtgaa
     8221 gttgtccttc cggggaagca cgatgggggc cagcgcctcc ggcgcggccg cgatcatgtg
     8281 gcgcgcgcgc tcttcgaagc cattgacttc acctggtgtg tagcgcagcg gacgacccat
     8341 ctggcgtgtg atggcctcgg ccgcctcggc cttgccggcc acgaaacggt cgacggcggc
     8401 cgataccgcg gcgcagcgtt cggccagggg gcgggcaccc cagtcgcgcc gcgccgcctt
     8461 ggcgcgctcg agcgcgcggg cgatgtcggc ctcgctggtg taggggcggc gcaggagttc
     8521 ccgcccgtct atggggctga tggtgacgag ttctgtggac atggcgagga tgtggacggt
     8581 ttgaggatga ttggaggccg ggcgtcgttt gaccaaacgg catacccggg attcaggcga
     8641 gccggttggg cttcaaatag cctgtggact catatgattt cgaaatagcg cttcagttcc
     8701 cagtcggtga cgtggcgtcg gaattcgcgt tcctcccatt cccgggtcgc ggcgaaatgc
     8761 tcgacgaacg gggcgccgaa caacgcacgc gccgtttcgg attgccgcaa gcgctgagcc
     8821 gcgtcccaca gcgtagcggg aaggcgcagg tgctcgggga aggtctgggc gtaggcgttg
     8881 ccggtgacca tggggccggg ttcgagtttc tgttcgatgc cggccaggcc gcttcccagg
     8941 gcggccgcca aggcgaggta cgggttggcg tcggctgaac cgatacgcac ttcgacccgt
     9001 tgcgattttt ccgagccggg aatgacgcgc agcgcggtgg tgcgattctc gatggcccag
     9061 gtggcgttca gcggcgccca gtagcccggc accagccggg agtagctgtt gatggtctgg
     9121 gcgtataggg ccatgaactc gggcaggtaa cgctgcaggc ccgccacgaa ctgcctgtgg
     9181 acggtactca tattgtaagg ctggctctcg tcgaagaagg cgttgcctcc ggattggcga
     9241 tcgcgcagcg acaagtggat atggccgctc tggcccggca ggtcgtgcga ccacttggcc
     9301 atgaatgtcg ccatcaggga atgctgctcc gccagcgcct tggtgaaggt cttgaacagg
     9361 aaccccttgt cggcggcctt ggcgatctcg tcgtaggcga tggccgcttc gagcacgccc
     9421 ggtccggttt cggtatgcag accttcgatg gacatgtcca ttttttcggt cagatcgatt
     9481 agttcggcgt agaagtcgga attgaccgtg ctgcgcaaca ccgagtagcc aaaatttccc
     9541 ggcgtccagg tttccaggtt ccgatagttt ttttcgcgca ccgacttcgg tgtttcattg
     9601 aacatgaaga actcatattc gagcgcggag aaggcatcca ggcccatgtc gcgcgccttg
     9661 tcgatcacgc gcttcagtag gcggcgtggg cagacttcgg cggcgcggcc atcgaattcc
     9721 gccagaaaga acagcatgtc gtggccgtcg gcgttttttt ccagaggcag gcgccggcac
     9781 gatacagggt ctatgcgcac ctgggcatcc gggtaggcgg tgtgccaacc ggtgaactgg
     9841 acgttgtcgt acatggcgtc gttggaatcc caacccatga cgacgtcgca gaacgccagt
     9901 ccggacttca acgcgccgag aaacttgtcc tggcgcacgt acttgccgcg catgacaccg
     9961 tcgatgtcgg acatgccaag cttgatgtgg gtgagaccgc tttccttgac caggcg
//
LOCUS       DASTNK010000140        16829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1225744, whole genome shotgun sequence.
ACCESSION   DASTNK010000140 DASTNK010000000
VERSION     DASTNK010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 16829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16829
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225744"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VFR20_10410"
     CDS             complement(<1..371)
                     /locus_tag="VFR20_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0230974.1"
                     /translation="MVRRSLRSVWHPCTQMQHHETVPLVPISHGRGAWLYDVDGKRYL
                     DAISSWWVNLFGHANPRINAAVAAQLEKLEHAMLAGFTHEPVIRLSECLAERTGNRLG
                     HCFYASDGASAVEIALKMSFHA"
     gene            complement(489..1667)
                     /gene="bioF"
                     /locus_tag="VFR20_10415"
     CDS             complement(489..1667)
                     /gene="bioF"
                     /locus_tag="VFR20_10415"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEU0230975.1"
                     /translation="MCDLIDETDTRLQQLQEKSLLRQRHVIDGPQGPLLMVDGKQYLA
                     FCSNDYLGLANHPALVAAARDGLDHFGVGASASALISGHSHVAEELEAALAAFVGLPR
                     ALYFCSGYMANLGVVPALVGRGDTIFSDRLNHACLIDGARLSSAEFRVYPHGDTVQLE
                     RRLAKCASPHKLILSDAVFSMDGDIAPLPELAALCERYDAWLLVDDAHGFGVLGPHGR
                     GSLAHFGLRSPRVLYMGTLGKAAGVAGAFVAGDATLIEWLMQRARTYVFTTASPPLLA
                     SALLAALALLRADDSRQRHLRGLVSQLRDGLSDLPWRLLPSESAIQALVVGENELALD
                     LMGALRERGIWVPAIRPPTVPKNTARLRISLSAAHTNEQVASLVDALKSVAASAMFDA
                     "
     gene            complement(1660..2919)
                     /locus_tag="VFR20_10420"
     CDS             complement(1660..2919)
                     /locus_tag="VFR20_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU0230976.1"
                     /translation="MMNSLFLDDPYPAYRAMRAAGPLHWSTEFCGGSWLLTHYTDVAS
                     VLRDPRFSVRRAGGWINGSGPAALVELREFKRIFPRSLLFTDAPRHTRLRRAVSAGFK
                     PFMLQSLAPRIERVVDALLGAVVASAEGPTGTFDFMRDFARPLPALVMAEMLGIDGAD
                     RARFVAWSDDIADFIGSPTPNMDVARRAQTGLSAMTGYFRDLLLQWRQRPGDDLISQL
                     IRAEADGGIMTANELLAQCCTLLFAGHETTRNLLGNGMLALLRHPAQWRALRREPALM
                     PLALRELLRFDSPVLYTGRRLKVDVELHGQWMRKGDLVIPLIGAANHDPAKFTAPDEL
                     DIRRDQGGHLSFGQGPHVCVGAALTYLEGDIALRGVMRYLPGLRLTDAPVSWRHNAVY
                     RGLDTLPLRFTSARNDSTVASKSMSCV"
     gene            complement(2964..3971)
                     /gene="bioB"
                     /locus_tag="VFR20_10425"
     CDS             complement(2964..3971)
                     /gene="bioB"
                     /locus_tag="VFR20_10425"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEU0230977.1"
                     /translation="MASDTNTFTRDTPPLWTIKDILGLYDLPLLDLLYQAQTVHRAHH
                     EANAVQLSSLLSIKTGGCPEDCSYCPQSARYDTGVEKDALMPLDEVVEAARQAKASGA
                     QRFCMGAAWRSPSARQIDDVAEMVSAVKGLGLETCVTLGMLKDGQAERLRDAGLDYYN
                     HNLDTSPEFYEKIITTRTYQDRLDTLERVQSAGIHVCCGGIVGLGESRTVCAGLIAQL
                     ANLAPYPESVPINNLVKVKGTPLYDAADLDPFEFVRTVAVARIAMPKAVVRLSAGREA
                     MDESTQALCFMAGAGSIFYGEKLLTTGNPGLLKDRSLFARLGLHAVTQTATHDAAAQA
                     V"
     gene            complement(4307..5353)
                     /locus_tag="VFR20_10430"
     CDS             complement(4307..5353)
                     /locus_tag="VFR20_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEU0230978.1"
                     /translation="MTSSSSLPASDIVLSLHQVEKRYTQPSDMVDRVGRLLRGRTQPR
                     SVLAVANVDLDIHRGEVIGVVGESGCGKSTLGRVIAGIFPATRGQVRYEGQAIDAMTS
                     VQRHAYELGVQMIFQDPYASLNPRMRVLDIIGEAPRVHGLVSRQDQEAYVCDLMSRVG
                     LDPAYRFRYPHQFSGGQRQRIGVARALALKPSVIVCDEAVAALDVSVQAQVLNLFIEL
                     RKEFNLTYFFISHNLGVVSHLSDRVAIMYLGRIVEIAPAETIFTHANHPYTQALIKEL
                     PRLSAEHRQFEPIKGELPSPLDPPPGCPFHPRCPHAMPRCREALPVLKDIGLREGVSG
                     GDRAVHLSACHLND"
     gene            complement(5350..6339)
                     /locus_tag="VFR20_10435"
     CDS             complement(5350..6339)
                     /locus_tag="VFR20_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0230979.1"
                     /translation="MATPSSSRDLLLDVQGLKTYFHTREGVIKAVDGVSLTLRAGEIL
                     GLVGESGSGKSITAFSLMKLVDEPGRIHADRLQFDGHDLLSLSDDQYRALRGAAMAMI
                     FQDPMMTLNPTLRIDTQMIEAVLAHDKVSRKAARARAVDALARVGIPSPEERLRVYPH
                     HLSGGMRQRVAIAIALLNAPRLIIADEPTTALDVTIQGQILYEVQKLCRESGTALIWI
                     THDLAIVSGLADRLAVMYAGRIVETGTTVELVDSARHPYTHGLIASIPSIDTRGGDLV
                     QIPGSTPSLLKLPEGCPFRTRCYRATDQCRQAPPVRDLSPTHQAVCWHPGAAQ"
     gene            complement(6348..7268)
                     /locus_tag="VFR20_10440"
     CDS             complement(6348..7268)
                     /locus_tag="VFR20_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0230980.1"
                     /translation="MTAVPTTQATSIEAPQERRWRVFAAQFLTSKLAVAGLVLLVLFL
                     VLAVFAPWIAPQNPYDIANLNIMESKLPPGSASMDGAMTYWLGTDGQARDIFSAILYG
                     MRISLVVGVVSVTVAFVIGAAAGLIAAYFGGRIEALLMRIVDIQLSFPSILVALILLA
                     ALGKGVDKVIYALIAVQWAYFARATRGAAIVERNREYVEAAKCVALSWPRILWRHIFP
                     NCLPPLMVIVTIDLAHAISLEAVLSFLGVGVPVTEPSLGMLISNGFQYMMSGMYWISF
                     FPGIALALLIIAINLVGDHLRDILNPRNTI"
     gene            complement(7329..8303)
                     /locus_tag="VFR20_10445"
     CDS             complement(7329..8303)
                     /locus_tag="VFR20_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0230981.1"
                     /translation="MLSAIVRRVLQTLLVVLVMSALVFVGLYLIGDPVNMLASPEATE
                     AQRELIRQSLGLNLPLWQQYFIFLGKAAHLDFGNSFLTGEPAMRLILQRMPATLELAT
                     VAMLVSMLVGIPLGIYAGLKPNSLGARSVMGGSVLGFSLPNFWVGLMLIMLFAVQLGW
                     LPAGGRGPTVAIGPLQLSVFTLGGLKALILPACTIAVAKCALIIRVTRAATRECLPQE
                     YIKFARAKGLPERRVLGVHLLKNILIPVITIGGLEYGQVFAFAVVTETVFSWPGMGKL
                     LIDSIVNLDRPVVVAYLMLAVVFLAFLNLVVDIAYAILDPRVRLGGAA"
     gene            complement(8308..9858)
                     /locus_tag="VFR20_10450"
     CDS             complement(8308..9858)
                     /locus_tag="VFR20_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0230982.1"
                     /translation="MLALALLGASGLAQARDLTIALRSEPTSMDPLFHALTSNLQLSE
                     TLFDPLVRTDKNAKPIPALAESWKVDGDVWTFKLRPNVKFSDGSPFTAEDVVFTYNRV
                     PKVPNSPSSYTLYLSKVASVKAVDPLTLQITTKGPAPVLLANLSQISIMSHKDASGSV
                     PEGKTTVDLNRGDGLVGTGPYKFESWSRGAKIVFARNPYYWGPKPVWDKVTYRPISNP
                     AARVAALLSGDVDMIEDPPTDDLPRLLKNKNLHVQQTPSVRVIYIALNQSKEPPNGMS
                     GTDGKNPLADKRVREALSLAIDRKAIVARIMGGVAKPAANLLAYPAFGTSKSLAEAPA
                     ANPAKARQLLAAAGYAKGFTVSLGSPAGRYTNDKETAQAVAAMWARVGVKTNVETMAP
                     SVFFQKRNSYAFSTYLGGWAATSGEMINPLKSLVLTRDKEHGLGTTNYSHYSNPGVDK
                     LVMEASRTLDDTKRSQILQKAGEDAMSDYAILPLHFELSVWAMKKDIRYPGRADQMTL
                     AQGVVPAK"
     gene            10227..11423
                     /locus_tag="VFR20_10455"
     CDS             10227..11423
                     /locus_tag="VFR20_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEU0230983.1"
                     /translation="MKLVEPIVEWQAEIAAIRRDIHAHPELAYQENRTSDIVAKQLEG
                     WGVETHRGLGVTGVVGVIRGQSDNGRALGLRADMDALPMQERNTFAHASTHEGKMHAC
                     GHDGHTAMLLAAVRYLAEHRNFDGTVYAIFQPAEEGFVGARKMIEDGLFTRFPMQAVF
                     GMHNWPGMPVGTFGVINGPVMASSNYFRITVKGKGAHAAMPHLGLDPIAAVVQMAQAF
                     QTIVTRNLDPLDPVALSITQIHAGSADNVIPESAILHGTLRTFSESALEMIRGRLESI
                     TRNICAAMGCEADFNFDHKYPPTINHPEETEFSAAVLADIVGEDHVDRAIPPSLGGED
                     FALMLKERPGCYVWIGNGQGDHRLPGHGEGPCMLHNASYDFNDELIPLGASYWVELAR
                     RWLAAT"
     gene            11602..12570
                     /locus_tag="VFR20_10460"
     CDS             11602..12570
                     /locus_tag="VFR20_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase"
                     /protein_id="HEU0230984.1"
                     /translation="MSKIVVIHENSTWVEPLRAAFGELELSHEEWFLSEGLLDLSGTP
                     PEGVFYNRMSASSHTRGHRYAPEYTAGVLAWLESHNRRVINDSRALRLEISKIAQYAA
                     LRRHGIRTPRTLAAIGTQNIVEAATKMDGSFITKHNRAGKGLGVHLFHSVEALKQHVD
                     SAAFEPSIDGITLIQEYIQAPEPFITRAEFVGGRFLYAVRVDTSQGFELCPADVCRID
                     DLACPVGGETAPSAPRFDIIEGFDHPIIERYQHFMADNGIGIAGIEFITDAHGDLYTY
                     DVNTNTNYNSDAESRAGLFGMRTIAAYLAEESRQLEESRLAFSVAA"
     gene            12926..13774
                     /locus_tag="VFR20_10465"
     CDS             12926..13774
                     /locus_tag="VFR20_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0230985.1"
                     /translation="MITLLISLVCSVAVSVLLKIARSRNLDMSQAIAVNYVLAAILCL
                     AILHPSDSSLLHPDTRWWVLIALGVLLPSVFLAMAGAVRHAGIVLSDAAQRLSLIIPL
                     TAAFLIFGEHGSTDKFLGIGMAFIALLCLLARPRQSEGSSETGRSIALLLCVWAGYGV
                     IDILFKQMARSGTSFSSSLFASFVLAGVLMFIYMIARRTRWTARSVVAGIILGALNFS
                     NIYFYLRAHQVFPHNPTLVFSAMNIGVISLGTVVGALIFRERLSWTNGAGIVLAIAAI
                     VMLFPH"
     gene            complement(13785..15140)
                     /locus_tag="VFR20_10470"
     CDS             complement(13785..15140)
                     /locus_tag="VFR20_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0230986.1"
                     /translation="MLTSIASFFSLYLTTLLLQTSSGLFNTYLGLRLTAASVSALWVG
                     SLIAGYYIGLVFGARIGHRLIIRVGHIRAYAATAAIAAVTVLSMMLIDNLWVWLGFRF
                     IAGIALVTQFMVIESWLNEQTENYQRGRIFGFYMAFSGLGTVCGQLSLTLFPSLNYQP
                     LVFVAMCSVLCLVPVALTRRLHPAVQTPAPIIIHYYVKRVPLSLTVLFMTGTITGSFY
                     GLAPVYAVKQSMTSDQVGIFLAAGVLSGLLAQWPIGWLADRVNRAGLIRVNTMILAVL
                     AIPLWGWWQFPFWALVVFSCAIGVLQFTLYPMGVAFANDNVEPERRVGLSAILFMVYG
                     LGACIGPLIVGELMRTVSANMFYAFLSFAALVLVIVVRPTRVTGDHLSEDAPTQFVIM
                     ADNLQSSPVASALDPRVDVRNDVSHDPVVEPVEPLVDSPAPGTAPLSDQPATGASKAA
                     A"
     gene            complement(15246..15752)
                     /locus_tag="VFR20_10475"
     CDS             complement(15246..15752)
                     /locus_tag="VFR20_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEU0230987.1"
                     /translation="MTIKVGDHLPDGTLSEFMETATEGCSVGPNNFQVADLVKGKTIA
                     LFAVPGAFTPTCSNRHLPGYLELHDQIKAHGVDEIWCVAVNDPFVMGAWGRDQGVKGR
                     VRMLADGSAEWTKKLGLEFDLTARGLGLRSQRYSAVIRNGVVDRLNVESAGQLEVSDA
                     KTLLGQLG"
     gene            16093..16605
                     /gene="moaC"
                     /locus_tag="VFR20_10480"
     CDS             16093..16605
                     /gene="moaC"
                     /locus_tag="VFR20_10480"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEU0230988.1"
                     /translation="MTTPDIAPANALALSHLDESGQIRMVDVGDKSPTRRCAIAQGRV
                     RMGAAAYRLLAAHENGKGEVLNTARVAGILAAKRCAELIPLCHSLPLSFAGIEFSLDD
                     AQCCIDIRATCRTDHTTGVEMEAMTACSVAALTIYDMCKALSHGIVIAETRLLEKQGG
                     KRDVRAGMAR"
     gene            16602..>16829
                     /locus_tag="VFR20_10485"
     CDS             16602..>16829
                     /locus_tag="VFR20_10485"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU0230989.1"
                     /translation="MTSAPASVYGLVLAGGASRRMGRDKAALELDGEPLLRRAVARLR
                     LALAEVIVVGAPTFAPLVPETRIVADDWPGRG"
BASE COUNT         3447 a         5421 c         5051 g         2910 t
ORIGIN      
        1 gcgtggaaac tcatctttag cgcgatctcc acggcggacg cgccatccga ggcgtaaaag
       61 caatgaccca gccgattccc ggtgcgctca gccaggcatt ccgacaggcg gatcaccggt
      121 tcatgcgtga atccggcgag catcgcatgt tcaagttttt ccagctgtgc cgcaaccgcc
      181 gcgttgatgc ggggattggc gtggccgaac agattcaccc accaagagct gatggcatcc
      241 agatagcgct tcccatccac gtcatacagc caggcgccac gcccgtgact aatcggcacg
      301 agcggaacgg tttcatgatg ctgcatctgc gtgcagggat gccagacgct tcgcaggctg
      361 cgccgcacca tgtccaactg ggatgctggt gatacattct ttaccaacgg cggctcctcg
      421 atgttctctt tccgttcata aaaagtggcg ggcttcatcc ggcgtaccta gtgcaatgtt
      481 tgacgccatc aagcgtcaaa cattgcacta gcagccacgc ttttcaaggc atccaccagg
      541 ctggcgactt gctcattcgt atgcgcggcg gacagggata tgcgcaagcg ggccgtgttc
      601 ttgggcactg tcggcggccg gatggcgggc acccagatac ctctttcacg caacgccccc
      661 atcaaatcaa gcgccaactc gttctctccg acgacgagcg cctgaatggc cgattccgac
      721 ggcaacaggc gccacggtag atcggacaag ccatcgcgca actggctgac caacccacgc
      781 agatggcgct ggcgcgagtc gtcggcccgc aacaaggcca gggccgccaa caaggcactg
      841 gccagcaaag gcggactggc ggtcgtgaaa acataggtgc gggcccgctg catcagccat
      901 tcgatcaatg tggcatcgcc cgccacgaaa gcccccgcaa cgcccgcagc cttgcccagc
      961 gtgcccatgt acagcacgcg gggagaacgc aagccaaagt gtgccaaact ccccctgcca
     1021 tgcggcccca gcacgccgaa accgtgcgca tcgtcgacca acagccacgc atcgtatcgt
     1081 tcgcagagcg ccgccaattc cggcaatggc gcgatatcgc cgtccatgct gaacacggca
     1141 tcactcagga tcagtttgtg gggactggcg catttcgcca ggcgtcgctc cagctgcacc
     1201 gtatcgccat gcggatacac ccgaaactcc gcgctcgaca aacgcgcgcc atcgatcagg
     1261 cacgcatggt tgagccggtc cgaaaatatc gtgtcgccgc gaccgaccag ggccggcacg
     1321 acccccaggt tggccatgta gccactacag aaataaagcg cccggggcag gccgacgaat
     1381 gccgccaaag ccgcttccag ctcttcagcc acatggctgt gcccgctgat cagcgccgac
     1441 gccgacgcgc cgacaccgaa gtggtcgagc ccatcgcgag ccgcggcaac cagtgcggga
     1501 tgattcgcca acccgagata atcgttgctg caaaacgcca gatactgttt tccatcgacc
     1561 atcaacaaag ggccttgcgg gccatctatg acatgccgct ggcgcagcag gctcttttcc
     1621 tgcaattgct gcaaccgggt atccgtttca tcgatcaaat cacacacaag acatactctt
     1681 tgacgccacg gtcgaatcat tgcgcgccga cgtgaagcgc agcggcagcg tgtcgaggcc
     1741 gcgatacacg gcgttatgcc gccaagacac gggcgcatcc gtcaatcgca gcccgggcag
     1801 gtatcgcatg acaccacgca gcgcgatgtc cccttccaga taagtcaacg ccgcaccgac
     1861 acagacatgc gggccctggc caaacgacag atggccaccc tgatcccggc ggatgtcgag
     1921 ctcgtccggc gccgtgaatt tcgctggatc gtggttcgcc gcgccaatca gcgggatcac
     1981 aaggtcgcct tttctcatcc attgcccatg cagttccaca tcgactttga gacgccgccc
     2041 tgtgtacagt acagggctgt cgaaacgcag caattcccgc aatgccaaag gcatcagtgc
     2101 gggctcccgc cgcaacgcgc gccattgcgc cggatgccgc aatagcgcca gcatgccatt
     2161 gccaagcagg ttgcgcgtgg tttcgtgacc ggcgaacaac agggtgcaac attgcgccaa
     2221 tagttcgttg gccgtcataa tgccgccgtc ggcctcggcc cgtatcagtt gactgatcaa
     2281 atcgtcaccg ggccgctggc gccattgcag aagcaggtct cgaaagtagc cggtcatggc
     2341 gctcagcccg gtttgtgcgc ggcgggcgac atccatattc ggcgtcgggc tgccaatgaa
     2401 atcggcgatg tcgtccgacc atgccacgaa ccgtgcacga tccgcgccat ctatgcccag
     2461 catctcggcc atcaccaacg ccggcagcgg cctggcgaaa tcccgcatga agtcgaaagt
     2521 gccggtcggg ccttcggccg aagccaccac cgcgcccaat agagcatcga cgactctttc
     2581 aatccggggg gccagcgact gcaacatgaa gggtttgaac ccagcggata cagcccgccg
     2641 caggcgagtg tgccgaggcg cgtccgtgaa cagcaatgaa cgcgggaaaa tccgcttgaa
     2701 ctcgcgcaac tccaccaatg ccgcgggccc actaccattg atccaaccac ccgcgcgacg
     2761 caccgaaaaa cgcggatccc gcaaaacact ggcgacatcg gtgtaatggg tcagcagcca
     2821 cgacccaccg cagaactccg tgctccaatg cagcggccca gccgcgcgca tggcgcgata
     2881 ggcgggatag ggatcatcga gaaataggct attcatcatc gaccggtttt tgatggcgac
     2941 aacaaccgag aacgtgattt cgttcagaca gcttgtgccg cggcgtcgtg cgtagcggtc
     3001 tgtgtcacgg catgcagacc caggcgcgca aacaaagacc ggtctttcaa caacccgggg
     3061 ttgcctgtcg tcaacaattt ttcgccatag aaaatcgaac ccgcgccggc catgaaacac
     3121 agcgcctgag tggactcatc catcgcttcc cggcccgccg acaaacgcac cacggctttc
     3181 ggcatggcaa tgcgggcgac ggccacggtt cggacaaact cgaaaggatc cagatcggcg
     3241 gcgtcgtaca gcggcgtgcc cttgaccttg accagattgt tgatcggtac cgattccgga
     3301 tacggcgcca gattggccaa ttgcgcgatc aagccggcgc acaccgtgcg cgactcgccc
     3361 aggccgacga taccgccaca gcacacgtgg atgcccgccg actgcacgcg ttcgagcgta
     3421 tcgagccggt cttgataggt gcgcgtggta atgatctttt cgtagaattc cggcgaggtg
     3481 tccaggttgt gattgtagta atccaacccc gcgtccctca gcctttccgc ctggccgtcc
     3541 ttcagcatcc ccaacgtgac acaagtctcc aggcccaggc ctttcaccgc gctcaccatc
     3601 tcggcgacat catcgatctg acgcgcgctg ggcgaacgcc atgcggcacc catgcagaag
     3661 cgctgcgcgc cactcgcctt cgcctgccgc gccgcctcga ccacctcgtc cagcggcatc
     3721 agcgcgtcct tttccacccc cgtgtcataa cgcgccgatt gcgggcaata ggaacagtcc
     3781 tcggggcagc cgcccgtctt gatcgatagc aggctggata gctgaacggc attggcctcg
     3841 tgatgcgcgc gatgaaccgt ttgcgcctgg tacagcagat caagcagggg caaatcgtac
     3901 agaccaagaa tatccttgat cgtccaaagt ggaggagtat ccctggtgaa agtattggtg
     3961 tcggatgcca tgacgtatgc tgcccctgtc tgtgtgttga accgcagtat ggtaggggtc
     4021 atcgtccggg ccacgcaatc ctcgactcga cgcaatggag tggggttggg cggcttgcaa
     4081 tggctacttt tcctaaattg tcattttcaa tattcaacct ccttcaacct atggttatct
     4141 cgcgaggttg gaaaaattgt attcaaatga tggggccggt catggtggcg cctcaataag
     4201 cccacctcgg caaaaacggt tcaaacagta aagcggttcg tattcagcac catgtacact
     4261 tcagccgaag atgctctccg gtcaaccaag tgaaagtcgt cttctctcag tcgttcaaat
     4321 gacaggcgct caagtgcacc gccctgtcgc caccagacac cccttcccgc aacccgatgt
     4381 ccttcagcac cggcaacgct tcccggcagc gcggcatggc atgcgggcag cgcggatgaa
     4441 acgggcaccc tgggggcggg tcgagcggcg agggcaattc cccctttatg ggctcgaact
     4501 ggcggtgctc ggccgacagc ctgggcagtt ctttgatgag cgcctgggtg tagggatggt
     4561 tggcgtgggt gaaaatagtc tcggccggcg cgatctcgac gatgcgcccg aggtacatga
     4621 tggcgacgcg atccgacaga tggctgacca cacccaggtt gtggctgatg aagaaatagg
     4681 tgaggttgaa ttccttgcgt agctcgatga acaggttcag gacctgggcc tgcacggaca
     4741 catcgagcgc ggccacggcc tcgtcacaaa caatgaccga cggcttgagg gccagcgcgc
     4801 gcgccacgcc gatgcgttgg cgctgaccgc ccgagaactg atgcggataa cggaaccggt
     4861 atgcggggtc caggccgacc cgggacatca ggtcacacac ataggcttcc tgatcctggc
     4921 gcgataccag gccatgcacg cgcggggcct caccgataat gtcgagtacc cgcatgcgcg
     4981 gattgagcga ggcgtacgga tcctggaaaa tcatttgcac gcccagctca taagcgtgac
     5041 gctgcacaga ggtcatggca tcgatcgcct ggccttcata acgaacctgc ccgcgtgtcg
     5101 ccggaaaaat accggcgatg acgcgcccca gtgtcgattt gccgcaaccc gactcaccca
     5161 ccacgccgat cacctcgcca cggtgaatgt ccagatcgac attggccacc gccagcacgg
     5221 atcgaggctg ggttcgacca cgaagcaacc ggccaacccg gtcgaccata tcggagggct
     5281 gcgtgtagcg tttctcgacc tgatgcaggc tgagcacgat gtcggacgct ggcagactgg
     5341 atgatgacgt cattgtgcgg ctccgggatg ccagcatacc gcctgatggg tgggcgaaag
     5401 atcccgcacg ggcggcgcct gccggcattg gtccgtggcg cggtaacagc gtgtgcggaa
     5461 cgggcacccc tcaggcagct tcagaagcga cggggtggat ccgggaatct ggaccagatc
     5521 gccgccacgg gtatcaatgg acggaatgga ggcgatcagg ccatgcgtgt aggggtgcct
     5581 ggcggaatcc acgagttcaa ccgtggtgcc ggtctcgacg atgcgccccg catacatcac
     5641 ggccaggcga tcggccagac ccgatacgat cgccaggtcg tgtgtgatcc agatcaacgc
     5701 cgtgcccgat tcccggcata gcttctgcac ctcgtagagg atctggccct ggatcgtgac
     5761 gtccagcgcg gtggtgggct cgtcggcgat gatcaggcgc ggggcgttga gcaacgcgat
     5821 ggcgatggcg acacgctggc gcatgccccc cgacaaatgg tgcgggtaca cgcgcaggcg
     5881 ctcctcgggc gacgggatgc ccacgcgcgc cagcgcatcc acggcgcgcg cccgcgcagc
     5941 cttgcgggac accttatcgt gcgccagcac ggcttcgatc atctgggtat cgatgcgcag
     6001 cgtggggttg agcgtcatca tcgggtcctg gaagatcatg gccatggcgg cgccgcgcaa
     6061 tgcccgatac tgatcgtcgg acagggaaag cagatcatgc ccgtcgaact gaaggcgatc
     6121 ggcatgtatg cggccaggct cgtcgaccag tttcatcagc gagaaagcgg tgatgctctt
     6181 gccagacccc gactcaccca ccaatcccag gatctctccg gcgcgcaatg tcagcgacac
     6241 gccatcgacc gccttgatca caccctcgcg ggtatggaag tacgtcttca gcccctggac
     6301 gtcgagcaaa aggtcgcgag atgaagaggg cgtggccatg attgacgtca gatcgtgttg
     6361 cggggattga ggatatcgcg caggtgatcc cccaccagat tgatggcgat gatcagcagt
     6421 gccagcgcga tgccggggaa aaacgaaatc cagtacatgc ccgacatcat gtattgaaag
     6481 ccattggaaa tgagcatgcc cagcgacggc tcggtcacgg gaacgccaac ccccaggaag
     6541 gaaagcaccg cctccagcga aatggcgtgc gccaggtcta tggtgacgat gaccatcaac
     6601 ggcggcaggc agttcgggaa gatgtgccgc cacaggatgc ggggccacga cagcgccacg
     6661 catttcgcgg cttccacgta ttctcgatta cgctcgacga tggccgcgcc acgtgtggcg
     6721 cgggcaaaat acgcccactg cacggcgatg agcgcgtaaa tgaccttgtc cacccccttg
     6781 cccagcgcgg ccaatagaat gagcgccacc agaatggatg ggaaagacaa ctggatgtcg
     6841 acgatgcgca tcagcaaggc ttcgatgcgg ccaccgaaat acgcggcgat cagccccgcc
     6901 gccgcaccga tgacgaacgc cacggtcacc gacaccaccc ccacgaccag gctgatgcgc
     6961 atgccgtaca gaatggccga aaaaatatcg cgggcctgcc cgtcggtgcc cagccagtag
     7021 gtcatggccc cgtccatgct ggcgctgcct ggcggcagct tggattccat gatgttcagg
     7081 ttggcgatgt cataagggtt ctgcggcgcg atccacggag cgaagaccgc cagcaccaga
     7141 aacagcacaa gcagcaccag gcccgcgacc gccagcttgc tggtgaggaa ctgcgcggcg
     7201 aagacccgcc agcgccgctc ctgtggcgct tcgatcgaag tggcctgcgt ggtcggaacc
     7261 gccgtcatga cgccctccgg tgacgaatgc gcggactcat gaggtacctc cgaggcggac
     7321 ggggggactc atgcagcacc tccgagacgg actcgtggat cgaggatcgc gtaggcgatg
     7381 tccacgacga gattcaggaa cgccaggaac accacggcca gcatcaagta ggccacgacg
     7441 accggacgat ccaggttgac gatggaatcg atcaacagct tgcccatgcc gggccacgaa
     7501 aacaccgttt cggtcacgac cgcgaacgcg aacacctggc cgtactcgag gccacctatc
     7561 gtgatgaccg ggatcaggat gttcttgagc agatgcacgc ccaggacccg gcgttctggc
     7621 aagcccttgg cgcgcgcgaa cttgatgtat tcctgcggca ggcattcacg ggtggcggcg
     7681 cgcgtgacgc ggatgatcag ggcgcacttg gccaccgcga tggtgcaggc cggcaggatc
     7741 aaggccttca atccacccag ggtgaacacg ctgagctgca gcggcccgat cgcgaccgtg
     7801 gggccgcgcc cgcccgccgg cagccagccc aactgcacgg cgaacagcat gatgagcatc
     7861 aggccgaccc agaaattcgg cagcgaaaag cccagcaccg atccacccat gacggaccgc
     7921 gcccccaggc tgttcggctt gagcccggcg tagatgccca gcggaatgcc cacaagcatg
     7981 gagaccagca tggccaccgt ggccagttcg agcgtggccg gcatgcgctg cagaataagc
     8041 cgcatggccg gctcgccggt gaggaagctg ttgccgaaat ccaggtgcgc cgccttgccg
     8101 aggaaaatga aatactgttg ccacagcggc aggttcaggc ccagcgactg acggatcagt
     8161 tcgcgctggg cctcggtggc ctctggactg gccagcatgt tcaccgggtc gccgatcagg
     8221 tacaggccga cgaacaccag cgccgacatg accagcacga ccagcagggt ctgcaggacc
     8281 ctgcgcacga tggccgacag catggcgcta cttcgccggc acgacgccct gcgccagcgt
     8341 catctggtcg gcgcgccccg gatagcggat gtctttcttc atggcccaca ccgacagctc
     8401 gaaatgcagc ggcaggatgg cgtagtcgga catggcatcc tcccccgcct tctgcaggat
     8461 ctgcgaacgc ttggtgtcgt caagcgtacg ggaggcctcc atcaccagct tgtcgacccc
     8521 cggattgctg tagtggctgt agttggtggt tcccagccca tgctctttgt cgcgcgtcag
     8581 gaccagggac ttgaggggat tgatcatttc gccggacgtg gccgcccaac cgcccaggta
     8641 ggtgctgaaa gcgtagctgt tgcgcttctg gaagaacacc gatggcgcca tggtttcgac
     8701 attggtcttg acacccacgc gcgcccacat cgccgccacg gcctgcgcgg tttccttgtc
     8761 gttggtgtaa cgacccgccg gcgacccgag tgaaacggtg aaccccttgg cgtaacccgc
     8821 ggcggccagc aactgcctgg ccttggccgg attcgccgcg ggcgcctcgg ccagggactt
     8881 ggaggtgcca aaagccggat aagccagcag gttggcggcg ggcttggcca caccgcccat
     8941 gatgcgcgcg acgatggcct tgcggtctat ggccagcgac agcgcctcgc gcacacgctt
     9001 gtcggccagc ggattcttgc catccgtgcc actcatgcca ttggggggtt cctttgactg
     9061 gttcagcgcg atgtagatga cacgcacgga cggcgtttgc tgcacgtgca ggttcttgtt
     9121 cttgagcagg cgcggcagat catctgtcgg aggatcctcg atcatgtcca cgtcgcccga
     9181 caacagcgcc gccacgcggg cggccgggtt ggagatggga cgataggtga ccttgtccca
     9241 caccggcttg gggccccagt aataagggtt gcgcgcgaag acgatcttgg cgccgcgcga
     9301 ccaggattca aacttgtagg ggccggttcc caccaggccg tcgccacgat tgagatccac
     9361 cgtggtcttg ccctcgggca ccgaaccgga cgcatccttg tgcgacatga tggaaatctg
     9421 cgacaggttg gcgagcagca ccggcgccgg gcccttggtg gtgatctgca gcgtcagggg
     9481 gtcaacggcc ttgacgctgg cgaccttgct gagatagagc gtgtacgacg acgggctgtt
     9541 cggcaccttg ggcacgcggt tgtacgtgaa cacgacgtcc tcggcggtga acggcgagcc
     9601 gtcggagaac ttcacattgg gacgcagctt gaatgtccag acatcgccgt ccaccttcca
     9661 ggattccgcc agcgcgggga tgggcttcgc gttcttgtcg gtgcgcacca gcggatcgaa
     9721 caacgtctcg gacaactgca ggttggacgt cagggcatgg aacagcgggt ccatggaagt
     9781 gggctcggaa cgcagggcga tggtcaggtc gcgcgcctgc gcgaggccgg aagcgcccag
     9841 cagcgccaat gccaacatgg acgacagggc cgtcttgcga atggatttca tcttggtttc
     9901 cctccttttg tccgtggcga tgccacggca ggcttgtcga aaatcaaaaa gccggcacac
     9961 gcaccggctt tggctcctcg aacacaacga taccttattt cagcgccaag tcagctgcga
    10021 gataacctgt atggtcggag gagaacaatg atcgacgagc aacttcgtta tcgcgcaacc
    10081 cgcatgaatt cacgctttac gaggaaccat tcgaaaacca ccacgacgcc tcatacgggt
    10141 aaacaccgat aacgcggccg cgaggtttga agaagggttt ccgatggcgt acagtcggga
    10201 gttccataac aacaggacaa ccctccatga agctcgttga gcccatcgtc gaatggcagg
    10261 ctgaaatcgc cgccattcgt cgcgatatcc acgcccaccc ggaactcgcc tatcaggaaa
    10321 accggacctc cgacatcgtg gcgaagcagc tcgaaggctg gggcgtggaa acacatcgcg
    10381 ggcttggcgt gacaggcgtg gtgggggtga ttcgtggcca atccgacaac ggccgcgccc
    10441 tgggcctgcg tgccgacatg gacgccctgc ccatgcagga acgcaacaca ttcgcccacg
    10501 ccagcacgca cgaaggcaag atgcatgcct gtggccatga cggccacacg gccatgctgc
    10561 tcgcggcggt gcgctatctg gccgaacacc ggaatttcga cggcaccgtc tacgccattt
    10621 tccagcccgc cgaggaaggc ttcgtcggcg cgcggaaaat gatcgaagac ggcctgttca
    10681 cacgtttccc catgcaggcc gtgttcggca tgcacaactg gccgggcatg cctgtcggca
    10741 ccttcggcgt catcaatggc ccggtcatgg cgtccagcaa ttatttccgc atcaccgtca
    10801 agggcaaggg cgcccatgcg gccatgcccc acctggggct cgaccccatc gcggccgtgg
    10861 tgcaaatggc ccaggcattc cagaccatcg tcacgcgcaa tctcgaccca ctggatcccg
    10921 tcgcgctcag catcacgcag atacatgccg gttccgccga caacgtcatc cccgaatccg
    10981 cgatattgca cggcactttg cgcacgttct cggaatcggc gctggaaatg atccggggcc
    11041 ggctcgaatc catcacgcgg aatatctgtg cggccatggg atgcgaggcc gacttcaact
    11101 tcgatcacaa gtaccccccc accatcaacc atcccgagga aaccgaattc agcgcggcgg
    11161 tgctggccga catcgtgggc gaagatcatg tggatcgtgc catcccaccc tcgctgggcg
    11221 gcgaggactt cgcactcatg ctcaaggaac ggccgggttg ctacgtgtgg atcggcaacg
    11281 ggcagggcga ccaccgcctg ccgggccatg gcgaaggccc ctgcatgctg cacaacgcca
    11341 gctacgactt caacgacgaa ctcatcccgc tgggggccag ctactgggtc gagctggcgc
    11401 gccgctggct ggcggcaaca tgaccaacac ggacccgaca cggccagccc gtcggaaccc
    11461 gcaccgaata accccgggca gaataatcat ataagcaata attcgtttgt cattcggcga
    11521 ccggtgacta agctgggatt ctccctgttt ccattgagcc ggccgtgccc accaggcccg
    11581 gatcgggttc gtctggtcgc catgtcaaaa atcgtcgtca tccacgaaaa ttccacctgg
    11641 gtcgaaccgc tgcgcgcggc gttcggcgaa ctcgaacttt cccatgagga atggtttctc
    11701 agcgagggcc tgctcgacct ttccggcacg ccaccggaag gcgtgttcta caaccgcatg
    11761 agcgcatcgt ctcatacccg gggccaccgc tacgcgccgg aatacacagc cggtgtcctg
    11821 gcatggctgg aatcacacaa ccggcgcgtc atcaacgaca gccgcgccct gcgcctcgag
    11881 atcagcaaga tcgcccaata tgccgcgctc aggcgccacg gtattcgcac cccgcgtacg
    11941 ctggcggcca tcggcacgca aaatatcgtg gaggccgcca caaaaatgga cggctcgttc
    12001 atcaccaagc acaaccgggc cggcaaaggc ttgggcgtgc atctgttcca tagcgtcgaa
    12061 gcgctcaagc agcatgtgga cagcgccgct ttcgagccct ccatagacgg catcacgctg
    12121 atccaggaat atatccaggc gcccgagcct ttcataaccc gggccgagtt tgtcggcgga
    12181 cgctttctgt acgcggtgcg cgtggatacg tcgcagggct tcgaactgtg ccccgccgac
    12241 gtctgccgca ttgacgacct ggcttgcccg gtgggcggcg agaccgcgcc atccgcgcca
    12301 cgattcgaca tcattgaggg attcgaccac cccatcatcg aacgctacca acatttcatg
    12361 gcggacaacg gcattggcat cgccggcatc gaattcatca ccgacgcgca cggcgacctc
    12421 tatacctacg acgtcaatac caacacgaac tacaacagcg acgccgaaag tcgggcgggc
    12481 ctgttcggta tgcgcaccat cgccgcctac ctggccgagg aatcgcgtca gctggaagaa
    12541 tccaggctag ctttttccgt ggctgcctga tacggccgac catccggcca ggcacggcgc
    12601 tgcgcgtccg gcaccacagc cggcgccagc gtcgtctgtg tcatagacac aataaagaca
    12661 ccccgcctcc agatcacaac gctctgccac gcaggataat tgtttcaatt tcatacaatt
    12721 tcaggagcaa tgggaattgt cgacaggttg actgccgttg cgcaccccac atcgcggaag
    12781 gcacggaagt tcagttaatc tgcgactatc tgacgcgaat tcagccgata aaagaacctg
    12841 cccccactat tgcgggccca catgccagcc cctaccatag ccggcaattc cgacaaactc
    12901 ccgagcctgc ctgagatccc gaacaatgat cacactgctc atcagccttg tctgcagcgt
    12961 ggccgtatcc gtcctgctga agatcgcacg cagccgaaac ctggacatga gccaggccat
    13021 tgccgtcaat tatgttcttg ccgccatcct gtgcctggcc attctgcatc cgtccgacag
    13081 cagtcttctg cacccggaca cacgctggtg ggtgttgatc gcgctgggag tgctgctgcc
    13141 atcggtgttc ctggccatgg ccggtgcggt gcgccatgcg ggcatcgtgc tcagcgacgc
    13201 cgctcaacgg ttgtcactca tcattccgct caccgccgcg ttcctgatct tcggcgaaca
    13261 cggctccaca gataaattcc tgggcatcgg catggcgttc atcgccctgt tgtgcctgct
    13321 ggcacgcccg cgtcaatcag agggttcgag cgaaacgggt cgatcgatcg cgctgttgct
    13381 ctgcgtgtgg gcgggatacg gcgtcatcga catcttgttc aagcaaatgg cccgcagcgg
    13441 cacatcgttc tccagcagcc tgttcgcgtc attcgtgctt gccggtgtgc tgatgttcat
    13501 ttacatgatc gcgcggcgca cgcgctggac ggcgcgcagc gtggtcgccg gcatcatcct
    13561 gggtgcgctg aatttcagca acatctattt ctatctacgc gcccaccagg tgttccccca
    13621 caatccgacc ttggtgtttt cagccatgaa catcggcgtc atttccctgg gtacggtggt
    13681 tggcgccctg atattccggg agcggctgtc atggacaaat ggagccggca tcgtcctggc
    13741 catcgccgcc atcgtcatgc tttttccgca ttagcgtaga acgctcaagc cgccgctttc
    13801 gatgcacctg tggcaggctg gtccgatagt ggcgccgtgc cgggggctgg cgaatcaacc
    13861 agcggttcga cgggttcgac caccggatcg tgcgatacgt cgttgcgcac atcgacgcgc
    13921 gggtccagcg cgctggccac cggggagctc tgcagattgt cggccatgat cacgaactgg
    13981 gtcggcgcat cctcgctaag gtgatcgccg gtgacacgcg tcggccgcac gacgatcacg
    14041 agcaccaacg cggcgaacga caggaacgca tagaacatgt tggcgctcac ggtgcgcatc
    14101 agttcgccca cgatcagcgg tccgatacaa gcccccaggc catagaccat gaacaggatc
    14161 gcgctcaggc ccacgcgccg ctcgggctcg acgttgtcat tggcgaacgc cacccccatc
    14221 ggatagagcg tgaactgcag cacaccaata gcgcaggaaa agaccaccag cgcccagaat
    14281 ggaaattgcc accagcccca gagcggaatg gccaacaccg ccaggatcat ggtgttgacg
    14341 cggatgagcc ccgcccggtt gacacgatcg gccagccagc cgatcggcca ctgggcgagc
    14401 agaccgctga gcaccccggc ggccaggaat atccccacct gatcgctggt catgctttgc
    14461 ttgaccgcat agaccggcgc cagaccgtag aacgagccgg tgatggtgcc ggtcatgaag
    14521 agcaccgtca atgacaacgg gacgcgcttg acgtagtaat gaatgatgat gggcgctggc
    14581 gtctgcacag ccggatgcag gcggcgcgtc agcgccaccg gcaccaggca cagcacggaa
    14641 cacatcgcca cgaacaccag gggttggtag ttgagcgacg ggaacaaggt cagcgaaagt
    14701 tggccacaca ccgtgcccag ccccgaaaac gccatgtaga agccaaagat gcgcccccgt
    14761 tggtaattct cggtctgttc gttgagccag ctttctatca ccatgaactg cgtgacgagg
    14821 gcaatgccgg cgatgaaccg gaaccccagc cagacccaga ggttgtcgat cagcatcatc
    14881 gacagcaccg tgaccgcggc gatggcggcc gtggccgcgt acgcccggat gtggccgacc
    14941 cggatgatga gccggtgccc gatgcgcgcg ccgaagacca ggccaatgta ataaccggcg
    15001 atcaggctgc cgacccacag cgccgagacg gaggccgccg tcaggcgcag gcccagatac
    15061 gtgttgaaca gacccgaact ggtctgcaac agcagcgtcg tgagatacag ggaaaaaaac
    15121 gaagcgatcg acgtgagcat ggattgtgcc ggggcggctc gcgcacgctc cccgattgtc
    15181 ggtccggaat gccacggccg gcagatgatg ccggccgtgc tgatcaagat acggggatgg
    15241 tcaggctacc ccagctgccc aagcaacgtc ttggcatcgc tgacctcaag ctgaccggcc
    15301 gactccacgt tcagcctgtc gaccacgccg ttgcgaatca cggcggaata ccgctgggaa
    15361 cgcagaccca gcccccgggc ggtgaggtcg aattccagcc ccagcttttt tgtccactcg
    15421 gcactgccgt cggccaacat gcgcacgcgt cccttgacgc cctgatcgcg cccccacgcg
    15481 cccatgacga acgggtcatt gaccgccaca caccagatct cgtccacgcc atgcgctttg
    15541 atctggtcat gcaattccag atagcccggc agatggcggt tcgagcatgt gggcgtaaaa
    15601 gcacccggga cggcgaacag cgcaatggtc ttgcctttca ccagatcggc aacctggaag
    15661 ttgttcgggc ccacgctgca gccttcggtg gcagtttcca taaactcgga caaggtacca
    15721 tcgggcaggt ggtcgccgac tttgatcgtc atggcagtct ccagagagat tgaaagaggt
    15781 tcgcccacag cgtgcgcggg caggaaatgt aatcatacga cgatgcggag atttatattt
    15841 tcgacgtggg ttttccatcc ccctgcctcc atcggctttt gccaaccgcc agccgaacca
    15901 gccgtcccgc cggcccggcg tcgatgccac gacagtgcgt ataatcaaga aaacagaccg
    15961 ccagatccaa gaaccccgcc ggattgcttt gaaaatacaa tcaggccagg tattaggctt
    16021 catgcgcgac cctgggattc aatctcgcgc tcccagccgg aaaccgtgcc ggctgccgta
    16081 tcctggacac ccatgactac ccctgacatc gctccagcca atgcgttggc cctaagccac
    16141 ctcgatgaat ccggccagat ccgcatggtc gacgttggcg acaagtcccc gacgcggcgc
    16201 tgcgccatcg cgcaggggcg tgtgcgtatg ggggccgccg cgtatcgcct gcttgccgcg
    16261 cacgagaacg gcaaaggcga agtgctcaac accgcccggg tggcgggcat cctggcggcc
    16321 aagcgctgcg ccgagctcat tcccctgtgc cacagcctgc ccttgagctt cgccggcatc
    16381 gagttctcgc tcgacgacgc gcaatgctgc atcgacatcc gcgccacctg ccgcaccgac
    16441 catacgacgg gggtggaaat ggaagccatg accgcatgca gcgtggcggc gctgaccatc
    16501 tacgacatgt gcaaggcgct ctcgcacggg atcgtgatcg ccgagacccg gctgctcgag
    16561 aagcagggcg gcaagcgcga cgtgcgcgcg ggcatggcgc gatgacgtcg gcgccagcat
    16621 cggtgtacgg cctcgtgctc gccggtggcg ccagccgccg catgggccgc gacaaggccg
    16681 cgctggagct ggatggcgag ccgctgctgc ggcgggcggt ggcacggctg cggctggcgc
    16741 tggccgaggt gatcgtcgtc ggcgcgccga ccttcgcgcc gctcgtccca gaaacgcgca
    16801 tcgtggcgga cgactggccg gggcgcggg
//
LOCUS       DASTNK010000141        19526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_299820, whole genome shotgun sequence.
ACCESSION   DASTNK010000141 DASTNK010000000
VERSION     DASTNK010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 19526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19526
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299820"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /gene="lysA"
                     /locus_tag="VFR20_10490"
     CDS             complement(<1..1206)
                     /gene="lysA"
                     /locus_tag="VFR20_10490"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU0230990.1"
                     /translation="MTPITHFPYRDSVLHAEGVPLSLIAREFGTPTFIYSRSALREAW
                     NAYSEAARGRNVLICYGMKANSNLAVLNEFKELGAGFDIVSGGELARVLAVGADPAKV
                     VFSGVGKQAWEMRAALEAGVKCFNVESEAELDRLAAVAHDVGKVAPVSLRVNPDVDAQ
                     THPYISTGLKENKFGIAIDRAMAAYQRAADAASLEVVGVDCHIGSQITEVGPYLDALD
                     KLLALIADLASRGIGLRHLDLGGGLGIRYKDEQPLAPHVLLDKVFERLEASGLGHLQV
                     VLEPGRSLVGNAGVLLTRVQYLKHNDVRNFAIVDAAMNDLLRPTLYRAWHDVIPVSQR
                     DPSTEPEQTYDVVGPICESGDWLARERKLAIRQDDLLAILSAGAYAFAMASQYNTRAR
                     AAEVMVDGDD"
     gene            complement(1226..1459)
                     /locus_tag="VFR20_10495"
     CDS             complement(1226..1459)
                     /locus_tag="VFR20_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016725145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEU0230991.1"
                     /translation="MKNATDPTITGYLCRIAGGLLLLSALGACGYKGPLYMPKPSPPP
                     ESLTKPPTPQPLPSETPPSTPASPGAATSSAVK"
     gene            complement(1602..4076)
                     /locus_tag="VFR20_10500"
     CDS             complement(1602..4076)
                     /locus_tag="VFR20_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU0230992.1"
                     /translation="MSSSANSSKTPSSASSPWVGRFLVKAIVFFGGLGLCGALLGALA
                     LALAWPNLPNLSAMTDYRPIIPLRVYTADKVLIGEFGEERRNVLRFNEIPDVMKSAIL
                     SAEDDKFYEHGGVDWSGVLRAAIANVIHMSKVQGASTITMQVARNFYLSSKKTYTRKF
                     YELLLTYKIEATLTKNQILDLYMNQIFLGHRAYGFAAACRTYFGKPLSEITAAEAAML
                     AGIPKAPSRFNPISNFKRAKERQAYVLGRMKALGYLTDAEYKKAMVQPIVVKSAAGTP
                     TGSYTVHGDYPAELARRLLYNVYQDNIYSRGFNIYTTINSKDQEAAYRAVRDGILDYT
                     RRSRYPGPVADIDLPTGIEKDPAKFDDLLDDLQDKYPDNGGLLTGVVLSASPSKVVVA
                     RSSQEIVDVDDKDALKVIARALKKNAKPTERLERGSVVYLHKNDGHWEIINMPTVEAA
                     FVAVRPQDGAILALVGGFNYGTDKFDRVTQAWRQPGSSIKPFIYASSLERGLTPATQI
                     SDEPFFLTAAQTGSKPWAPKNYGNHYEPMLTMREGLYKSENMVAIRIMQAVTPKYAQE
                     YITRFGFDKSRQPAVLPMAIGAGTVTPLQMAGAYSVFANGGYRVPPYLIDHVTDSSGH
                     VIMQAKPVIAGDAAARAIDPRTCYVMDDLLRGVATYGTGARAHRVLKRNDVGGKTGTT
                     NDSRDAWFAGFTPKTVGIAWMGYDHPRSLGMGETGGGAALPIWLDFMKVALKGQPETP
                     PGPMPEGLSKINGDYYFDEFPPGKAVARVGLPSSSDVSPNGSGGGGGQVDGIGALLNQ
                     LGSNSPAPAPTPSSNSGAAQPLPVHF"
     gene            4312..4809
                     /locus_tag="VFR20_10505"
     CDS             4312..4809
                     /locus_tag="VFR20_10505"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU0230993.1"
                     /translation="MMGAGKTTIGRQLARLLGREFIDLDQELEARCGVKVSLIFEIEG
                     EAGFRRRESQLLEECTRRRDIVLATGGGAVIAPENRRYLKERGVVVYLRATGPELYRR
                     VARDRNRPLLQTADPRARIDSLLAQRGPLYDEVAGITLETGAMPVSHAVRELLPLLQN
                     HKISA"
     gene            4806..5888
                     /gene="aroB"
                     /locus_tag="VFR20_10510"
     CDS             4806..5888
                     /gene="aroB"
                     /locus_tag="VFR20_10510"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU0230994.1"
                     /translation="MITVHVETPGGRYPIHIGQGRLDRLADSLPADATAFALVTNTTV
                     APLYAQRVEQALGQAGKRVVRIELPDGEVYKDWRTLNLIFDGLLRNGLDRKAVLVALG
                     GGVVGDMCGFAAASYMRGVRFLQVPTTLLAQVDSSVGGKTAINHPLGKNMIGAFHQPV
                     AVEIDTDALATLPDRELSAGLAETIKYGMIADADFFDWCEANMLALRRRDPDAIVTAI
                     RRSCELKAWVVSQDERESGLRAILNFGHTFGHAIEAGLGYGEWLHGEAVGCGMVQAAE
                     LSARVLGLPRTDVDRVRALVDAAGCPVLAPDLGTQQWLDHMRVDKKTEGGELRFVLLP
                     RIGQAEVRSVPAAPLADVLDSTVARA"
     gene            5888..7012
                     /locus_tag="VFR20_10515"
     CDS             5888..7012
                     /locus_tag="VFR20_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEU0230995.1"
                     /translation="MAPAPYACRPDQSRGRLHDEAPPENRTQFQRDRDRIVHCNAFRL
                     LEYKTQVFVNHEGDLFRTRLTHSIEVAQIARTLARNLGLNEDLVEAISLAHDLGHTPF
                     GHAGQDELNACLRELAPEAGGFEHNLQSLRIVDELEERYAAFNGLNLCFETREGILKH
                     CSLKYARRLGAVGERFIERTQPSLEAQVANLADEIAYNNHDVDDGLRSGLITLDQLQM
                     VEIFATHHSRVRRRYPNLDKRRAIAETVRAMINTLVLDLTETTLANIRQVAPASADDV
                     RRAAPLAAFSVSVRRQADALKQFLLDNLYRHYQVIRMTSKARQIVRELFVAFMNDPRL
                     LAEDHRRRDDVEQARAIADYIAGMTDRYAIREHQQLFQMR"
     gene            complement(7414..8748)
                     /locus_tag="VFR20_10520"
     CDS             complement(7414..8748)
                     /locus_tag="VFR20_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0230996.1"
                     /translation="MSQSHAPSSSIRKVVASSLIGATIEWYDFFLYGVIAGLVFNKQY
                     FPSHDPVVGTLLAYATFAVGFVARPFGGVVFGHFGDRIGRKSTLIFTLVLMGVATFLI
                     ALIPTYGSIGIWAPILLLLCRVVQGLGLGGEWGGAVLMTYEYAPEHKRGLYASLPQIG
                     LSIGLCLAAGVVALLSGTLSNAAFMSWGWRVGFMLSAALVFVGLYIRLTIKETPEFEQ
                     VKRENAELQIPFVDMAKRYPGNILKGMGARYIDGVFFNIFAVFSINYLTQTIKINRTD
                     ALTGVMAAAVVMCFFIPFFGALSDKVGRTRTYFWGSLITAASAWPAFWLMTNHSGNIL
                     LIWLSIVIPFGIFYAAIYGPEAALFCELFDTKVRYTGISFVYQFSGIFASGITPLIAT
                     ALLKLNGGQPWLIVAYTIFAGVVSALSAWLIGRSSSPQMAAPRAVARGARGI"
     gene            complement(8873..10486)
                     /locus_tag="VFR20_10525"
     CDS             complement(8873..10486)
                     /locus_tag="VFR20_10525"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEU0230997.1"
                     /translation="MPNTVDFIVIGAGSAGCVLANRLSANGKYSVCLIEAGPPDRNPW
                     IHIPIGYGKTMFHKTLNWGFQTDPDDNMLGRRIYWPRGRTLGGSSSINGLIYIRGQKQ
                     DYDHWEQAGNPGWSWEKCLPYFRKLENNDLGAGPTHGTDGPLNATSIKTSHPLVEALI
                     QSGATLGLKKRRDFNDGNQEGVGYYQLTTRNGKRCSTAVAYLVPARNRPNLRVETNAQ
                     VTAVVMEGTRAAGVRYRKNGQEHVLLANKEVILSAGAIQSPQLLQLSGIGPADLLGRF
                     GIPVVKNLSGVGQNLQDHLQIRLIYEVTQPITTNDQLRTIWSKARIGLQWLLFRGGPL
                     AIGINQGAVFCRALPEESPTPDSQFHFGTLSADMAGGAVHDFSGCTYSVCQLRPESRG
                     QINIRSADPLEAPSIQPNYLSSELDQRTAVAGFKYARRLAGAAPLRQLMKREYRPGDR
                     VRTDDEILHFCREYGATIFHPAGTAKMGSASDPGAVVDHRLRVHGVEGLRVVDASVMP
                     TLVSGNTNVPVVMIAERASDFILEDAQAA"
     gene            10757..11527
                     /locus_tag="VFR20_10530"
     CDS             10757..11527
                     /locus_tag="VFR20_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU0230998.1"
                     /translation="MSHPMIRYLRIPALLEQLARARHPQTLAQLAQRLDLPKTSLMRI
                     LSSLVESGFVVHAPGERGFVLGPRATRLALEVLGTPHFSRSSRAILARLVEATGETCN
                     LTALEGDGMRYLERVETGHLLRLKLDVGTHVPLHCTASGKLMLAMMDDAQRRQVLDRI
                     ALDRHTPKTLTDRVLLERELATIRQRRVGVDQEEFVLGMVAVSVPVLNATGGMIAAVA
                     CHGPTARISMAFLLESVPVMREAALAMGKVLGDDAMVW"
     gene            complement(11610..14486)
                     /gene="uvrA"
                     /locus_tag="VFR20_10535"
     CDS             complement(11610..14486)
                     /gene="uvrA"
                     /locus_tag="VFR20_10535"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU0230999.1"
                     /translation="MHAKPKEAIRIRGARTHNLKNISIDLPRHQLVVVTGLSGSGKSS
                     LAFDTLYAEGQRRYVESLSAYARQFLQLMDKPDVDLIEGLSPAISIEQKAAGHNPRST
                     VGTITEIHDYLRLLYARVGTPYCPEHGLPLQAQSISQMVDKVLAWAPDTRLAVLAPLA
                     RDRKGTFEEECARLQAQGFVRLRVNGDMVELDRLPPLQKNQKHDIDVVVDRLRNRPAS
                     RQRLAESLETAAGLADGRTTVLNMDSSEEQTFSSHYACPICGHSLTELEPRLFSFNNP
                     VGACPDCDGLGHVDFFDPKRVVAFPELSLASGAISGWDRRNTFSYTVLGSLAAHYGFD
                     LETPFQDLSEDARQRVLYGSGKEEISFLYMTEKGRPVTRKHPFEGVIPNLERRWKETD
                     SSTVREELGKLRHTKVCPACEGSRLRAEARNVLIGNDDGPGERRGRAIFEVEALPLSA
                     CLGWFEALELEGGKRDIAERIVREIAARLEFLNNVGLSYLSLDRSADTISGGEAQRIR
                     LASQIGSGLTGVMYVLDEPSIGLHQRDNDRLIETLKHLRDLGNSVIVVEHDEDMIRSA
                     DFVVDMGPGAGEHGGEIVAHGTPDDLARMEQSLTGQYLSGARRIAIPPRRPVTADLPR
                     LEIKGCRGNNLKSVDLAIPAGRLVCVTGVSGSGKSTLINDTLATAVAQRLHRAQTEPA
                     PYDSIDGLSHFDNIITIDQSPIGRTPRSNPATYTGMFTPIRELFAGVPEARTRGYGPG
                     RFSFNVKGGRCEACQGDGVVKVEMHFLPDMYVPCDVCNGRRYNRETLDIRYHGHNISD
                     VLELTVEQALAHFSAVPAIARKLNTLMDVGLSYIRLGQNATTLSGGEAQRVKLSQELS
                     KRSTGRTLYILDEPTTGLHFQDIAQLLDVLRQLVDAGNTVVIIEHNLDIIKTADWVVD
                     LGPDGGDAGGTLVAQGTPEDIAACAASHTGRYLKPLLVTPHNPR"
     gene            14799..16007
                     /locus_tag="VFR20_10540"
     CDS             14799..16007
                     /locus_tag="VFR20_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0231000.1"
                     /translation="MTNHTAGEGHSRRTRLKLNPVERRASVTLALLFAARMLGLFLLT
                     PVFAVAALSIPGGHDATKVGLALGAYGLTQAILQVPLGMASDRYGRRPIIVAGLLLFI
                     VGGVICALAPNIDWIIVGRVVQGLGAVSAAITAWVADATRPEVRTRAMAMVGGSIGIA
                     FAVSLVLSPLLVGAFGLSGLFWAISLLGFVCLLIATFVVPVVTPSETPIVQASARDVL
                     HHGDLLRLNFGVYCLHFTLMSIFMVAPGLLQHLGGYTESHLWEVYLPVILVSFVLMVP
                     AVFYTETRHAHRLALQVSVAGLVVVLGLLPLASHGFYTLVILMVGFFIAFNILEALQP
                     SLVSRVAPPEFKGLALGFYNTAQALGVFSGGVVGGVLAQHAGTSEVFLVAAVLLFAWF
                     LVARGFKYGG"
     gene            16248..16730
                     /gene="ssb"
                     /locus_tag="VFR20_10545"
     CDS             16248..16730
                     /gene="ssb"
                     /locus_tag="VFR20_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU0231001.1"
                     /translation="MASVNKVILVGNLGRDPEVRYSPDGAAICNVSIATTSQWKDKAS
                     GEKREETEWHRVVFYNRLAEIAGEYLKKGRSVYIEGRLKTRKWQDKETGADRFSTDIV
                     ADQMQMLGGRGEGGDFGGTEYGGAAPQPRQQSQQRAAAPRPAAAPATNLADMDDDIPF
                     "
     gene            complement(17007..18245)
                     /locus_tag="VFR20_10550"
     CDS             complement(17007..18245)
                     /locus_tag="VFR20_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0231002.1"
                     /translation="MTYPDNTAAINRPAPLSDAAQRANIWRLSIAQALAGANSVVTYA
                     TGAIVGDQLAPTPMLATLPISIFVIGMAACTLPVGAIARRHGRRTAFLAGTSTGVLAG
                     LFAALAVVMGWFWLFCLATLLGGAYAAVVLTFRFAAADGVAPGKRARALSLVMAGGVA
                     AGVFGPQLVTWTMDLWPPHRFTATFFIQAAVAAISAFVLLGVRLPTPTRTEIAAGRPL
                     IEIVRQPRFIAAAVCGAVSYLLMNFLMTAAPLAMHMTGHSQTSANNGLQWHVVAMYAP
                     SFFTGGLITRFGAGRVSLTGMALIGLAAVAGLGGTDVTHFWLALILLGMGWNFGFLGA
                     SALVLECHRPEEKTRVQSFNDFIVFGLMTIGSLASGALLTAFGWDMVLWVSFVPLALA
                     VGVLMVARRGGTGRVKREAV"
     gene            complement(18352..19341)
                     /locus_tag="VFR20_10555"
     CDS             complement(18352..19341)
                     /locus_tag="VFR20_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0231003.1"
                     /translation="MQYTKLGSTGLTVSRLCLGTMTFGLQIEEAAARAILDESAAQGI
                     TFIDTADVYPLGGDLHTAGRTEEIVGRWLKGRRDDFVLATKAVGRMGPHEWDLGASRK
                     HLISAIDASLRRLDTDYVDLYQLHSDDQTTPLDETLEALDAIVRSGKVRYIGVSNFLA
                     YRLAKALGRSDARNLARLVCVQPRYNLLFRQIERELLPLCAEEKLGVISYNPLAGGLL
                     TGKHHRDTKPEGGRFTLGTAGGMYQKRYWHEREFATVSQLVDAVRDSGLSLTTAAVAW
                     VLANPAVTSAIIGASRPEQLKDVIAAAETTLDNNLKAHLDELTHDYRMGDAAR"
BASE COUNT         3319 a         5980 c         6539 g         3688 t
ORIGIN      
        1 gtcgtcgcca tcgaccatga cttcggcggc gcgggcgcgt gtgttgtatt ggcttgccat
       61 ggcgaaagcg taggctccgg cggacaggat ggccagcaga tcatcctgcc ggatggccag
      121 tttgcgttcg cgcgcgagcc aatcgccgct ttcacagatt ggtccgacca cgtcgtaggt
      181 ctgttcgggt tcggtcgagg ggtcgcgttg agataccggg atcacgtcat gccacgcccg
      241 gtaaagcgtc ggccgcaaca gatcgttcat ggcggcgtcg acaatggcga aattgcggac
      301 gtcgttgtgt ttgaggtact gcacccgggt cagcagcacg ccggcgttgc cgaccagtga
      361 gcgtccgggc tccaacacca cctggaggtg tcccaggccg cttgcctcga gacgctcgaa
      421 caccttgtcc agcagcacat ggggcgcaag cggttgttcg tccttgtacc ggatgcccag
      481 tccgccgcca agatccagat gccgcaggcc gataccgcgg gaggccaagt cggcgatcag
      541 tgccaggagc ttgtccagag cgtccaggta cgggccgacc tcggtgatct gcgagcctat
      601 gtggcagtcg accccgacca cttcgaggct ggcggcatcg gccgcgcgct gatacgccgc
      661 catcgcgcga tcgatggcga tgccgaactt gttttccttg aggccggtgg agatgtacgg
      721 gtgggtctgg gcgtccacgt cgggattgac gcgcagggac accggagcga ccttgcccac
      781 gtcgtgggcc acggcggcga gacggtcgag ctcggcctcg gattcgacgt tgaagcactt
      841 gacccccgct tccagggcgg cccgcatttc ccatgcctgc ttgccaacgc ctgaaaacac
      901 cactttggcc gggtccgcgc caacggccag gacgcgtgcg agttcgcccc cggagacgat
      961 gtcgaagccg gcgcccagtt ccttgaattc attcaggacg gcaagattgg aattggcctt
     1021 catgccgtag cagatcagca cgttgcggcc ccgtgccgct tcgctatagg cgttccaggc
     1081 ttcgcgcagc gcactgcggg aatagatgaa cgtcggagtg ccgaattcgc gggcaatcaa
     1141 cgacaacggc acaccttcgg catgcagtac ggagtcacgg taggggaagt gcgtgatagg
     1201 agtcatggac atcgcgggtt gcgtgttact tcacggcgga agaggtggcc gcgccggggc
     1261 tggccggcgt cgatggtggg gtttccgaag gcagcggttg cggtgtgggc ggtttggtca
     1321 gcgattcggg cgggggcgag ggtttgggca tgtacaacgg ccccttgtag ccgcaggctc
     1381 ccagcgctga cagcagcagg aggccgcccg cgatgcggca caagtatccg gtgattgtcg
     1441 ggtcggtggc gtttttcatg gcgaggctta tggaatgtcg tgggggtgtc ggaatggtaa
     1501 aaagcccggc ggagccaggc tttttttggt gaaaatgagg cacggggcgg agtggagcgc
     1561 cggtaccagt ggcctgtgga gtgacaggcc actagcttac attaaaaatg caccgggagc
     1621 ggctgcgccg cgccactgtt cgaagagggg gttggcgccg gcgccgggct gttcgagccc
     1681 aactgattca gcaaggcgcc aatgccgtcg acctgtccgc ccccgccccc actcccgttg
     1741 ggcgagacgt ctgacgatga aggcaggccg acgcgggcca cggccttgcc cggcgggaat
     1801 tcatcgaagt agtagtcacc attgatcttg ctcaggcctt cgggcatcgg gcccgggggc
     1861 gtttccggtt gccccttgag cgccactttc atgaagtcga gccagatcgg cagggccgcg
     1921 ccgccaccgg tttcgcccat gcccagcgaa cggggatggt cgtagcccat ccaggcgatg
     1981 ccgacggtct tgggtgtgaa gccggcgaac caggcgtcgc gcgaatcatt ggtcgtaccc
     2041 gtcttgccgc ccacgtcgtt gcgcttgagc acgcgatgcg cccgcgcacc ggtgccgtac
     2101 gtggcgaccc cgcgcagcag gtcgtccatg acgtagcagg tgcgtgggtc gatggcgcgc
     2161 gctgcggcgt cgcccgcgat gaccggcttg gcctgcatga tgacatggcc gctgctgtcg
     2221 gtgacgtgat cgatgaggta gggcggcaca cggtagccgc cgttggcgaa gaccgaatag
     2281 gccccggcca tttgcaacgg cgtgactgtg ccggcaccga tcgccatggg cagcaccgcc
     2341 ggttggcgtg atttgtcgaa gccgaagcgt gtgatgtatt cctgcgcgta cttgggcgtg
     2401 accgcctgca tgatgcggat cgccaccatg ttctcggact tgtacagacc ctcgcgcatg
     2461 gtgagcatgg gctcgtagtg attgccgtag ttcttcggtg cccagggctt ggagccggtc
     2521 tgcgccgcgg tgaggaagaa cggctcgtcg gagatttgcg tggccggcgt caggccgcgc
     2581 tcaagtgacg atgcgtagat gaacggcttg atgctggaac ccggctggcg ccaggcctgg
     2641 gtgacgcggt cgaacttgtc ggtgccgtag ttgaagccgc cgaccagcgc caggatggcg
     2701 ccgtcctggg gtcgcacggc gacgaacgcg gcctcgaccg tgggcatgtt gatgatttcc
     2761 cagtggccgt cgttcttgtg caggtacacc accgagccgc gctcgaggcg ttccgtgggt
     2821 ttggcgtttt tcttcagcgc tcgggcgatg actttcagtg catccttgtc gtcgacgtcg
     2881 acgatttcct gggacgagcg cgccaccacg actttgcttg ggctggccga gaggaccacg
     2941 cccgtgagca ggccgccgtt gtcggggtat ttgtcctgga ggtcgtcgag aaggtcgtcg
     3001 aacttggctg ggtctttttc tatgccggtg ggcaggtcga tgtcggcgac ggggcccgga
     3061 tagcgcgacc ggcgcgtgta gtccaggatg ccgtcgcgca cggcgcggta ggccgcttcc
     3121 tggtctttcg agttgatcgt ggtgtagatg ttgaaaccac gcgagtagat gttgtcctgg
     3181 tagacgttgt acagcagacg gcgcgcgagc tcggccgggt agtcgccatg gacggtgtac
     3241 gagcccgttg gcgtgccggc cgccgacttg acgacgatgg gctgcaccat ggctttcttg
     3301 tattcggcgt cggtcaggta gcccagcgcc ttcatgcggc ccagcacgta ggcctggcgt
     3361 tccttggccc gcttgaagtt ggagatgggg ttgaagcgcg acggcgcctt gggaatgccg
     3421 gccagcatgg cggcttcggc ggcggtgatt tcagacagcg gcttgccgaa atatgtgcgg
     3481 caggcggccg cgaacccgta ggcccggtgc cccaggaaga tctggttcat gtacagatcg
     3541 agaatctggt tcttggtgag ggtggcctcg atcttgtagg tgagcagcag ttcgtagaac
     3601 ttgcgggtgt aggtcttttt cgacgacagg tagaagttgc gcgccacctg catggtgatg
     3661 gtgctggcac cctgcacttt cgacatgtgg atgacgttgg cgatggccgc gcgcagcacg
     3721 ccggaccagt ccaccccgcc gtgctcatag aacttgtcgt cctcggccga caggatggcc
     3781 gatttcatga catcggggat ttcgttgaag cgcagcacgt tgcgccgctc ttcgccgaat
     3841 tcaccgatca ggaccttgtc ggcggtgtag acgcgcaacg gtatgatcgg gcggtagtcg
     3901 gtcatggccg acaggttcgg caggttcggc caggccagcg cgagcgccag ggcgcccagc
     3961 agcgcaccgc acagtccgag ccccccgaaa aacacgatag ccttcacgag aaagcggccc
     4021 acccaggggc tggaggcgga gctgggcgtc ttggaagaat tggcagagga gctcatcggg
     4081 ggggatttta agtccaactc acgttagacc aggaattgtg cactgggttt ctgtaaccga
     4141 atactgcaat gttgcggtca tgcatcggat catgcatcga atccctaatg cgataataca
     4201 caaatggaaa agtgttcagt taccccggcc gcgccgcctg cgacagacac gccggtcgat
     4261 gacatcgacg cggtgatctt gccgttcgac ctgccggtct tcctggtggg aatgatgggc
     4321 gccggaaaga ccaccatcgg gcgtcaactg gcgcggctgc tggggcgcga attcatcgac
     4381 cttgaccaag agctcgaggc ccgttgcggt gtgaaagtat cgctgatatt cgagatagaa
     4441 ggcgaggccg gcttccggcg ccgcgaatcg caattgctcg aagagtgtac cagacgccgc
     4501 gacatcgttc tggcgacggg cgggggggcg gtcatcgcgc ccgagaaccg gcgctacctg
     4561 aaggagcgtg gcgtggtggt gtacctgcgc gccaccggcc ctgaactcta ccggcgcgtc
     4621 gcgcgcgatc gcaatcgccc actgttgcaa acggccgatc cgcgcgcgcg catcgacagc
     4681 ttgctcgcac agcgtgggcc gctctatgac gaagtggccg gcatcacgct cgaaaccggc
     4741 gccatgccgg tgtcgcatgc cgtgcgcgaa ctccttcccc tgctgcaaaa ccacaagata
     4801 tccgcatgat cacggttcac gtcgagaccc ccggggggcg ttatcccatt catatcggcc
     4861 agggccggct ggaccgcctg gcggacagcc tgccggccga tgccacggcc ttcgccctgg
     4921 tcaccaacac gaccgtggcg ccgctgtatg cgcaacgcgt cgaacaggcg ctcgggcagg
     4981 ctggcaagcg tgtggtgcgc atcgaactgc ccgacggcga ggtctacaaa gattggcgga
     5041 cgctgaatct cattttcgat ggcctgttgc gcaacggcct ggatcgcaag gccgtgctcg
     5101 tggcactggg cggtggcgtg gtgggcgaca tgtgcggctt cgccgcggcc agctacatgc
     5161 gcggggtgcg ttttctgcag gtgcccacca cgctgctggc ccaggtcgac tcctccgtgg
     5221 gtggcaagac ggccatcaac catcctctgg gcaagaacat gatcggcgcg ttccaccagc
     5281 cggtcgcggt ggagatcgac accgacgcgc tggccacgtt gcccgatcgt gagctttccg
     5341 ccggcctggc cgaaaccatc aagtacggca tgatcgccga cgccgatttt ttcgattggt
     5401 gcgaagccaa catgctggca ttgcgccgcc gcgatcccga cgccatcgtc accgccatac
     5461 gccgctcatg cgaactcaag gcctgggtgg tgtcgcaaga cgaacgcgaa tcgggcctgc
     5521 gcgccattct caattttggt cacactttcg gccatgccat cgaggccggt ctcggttacg
     5581 gtgaatggct gcacggcgag gccgtgggct gcggcatggt gcaggcggcg gaactttccg
     5641 cccgcgtgct gggtctgccg cgcacagatg tcgatcgtgt ccgcgccctg gtcgatgccg
     5701 ccggttgccc cgtgctggcg cctgatctgg gcacgcagca gtggctggac cacatgcgcg
     5761 tggacaagaa aaccgaaggc ggtgaattgc gcttcgtgct gctgccgcga atcggccagg
     5821 ccgaggtccg gtcggtgccg gccgcgccgc tggccgacgt cctggatagc acggtcgcgc
     5881 gggcgtgatg gctcccgccc cttacgcctg ccggcctgat caatcgcgtg ggcggctgca
     5941 cgacgaggcg ccgcccgaga accgcaccca gttccagcgc gatcgcgatc gcatcgtcca
     6001 ttgcaatgcc ttccggttgc tggaatacaa gacgcaggtg ttcgtcaatc atgagggcga
     6061 tctgttccga acgcgcctta cccacagcat agaagtggcg cagatcgcgc gcaccctggc
     6121 gcgcaatctg gggctgaacg aagacctggt cgaagccatt tcactggcgc acgacctggg
     6181 ccacacgccg ttcggccatg ccggccagga tgagctcaac gcctgcctgc gcgagctcgc
     6241 gcccgaggca ggtggtttcg agcacaatct gcagagcctg cgcatcgtcg acgaactcga
     6301 ggaacgctat gcggccttca atggcctgaa tctatgcttc gagacccgcg aggggatact
     6361 gaagcattgt tctctgaagt atgcgcgacg ccttggcgcc gtgggcgagc ggttcattga
     6421 acgcacgcaa ccgtcgcttg aagcgcaggt cgcgaatctc gcggatgaga tcgcctacaa
     6481 caatcatgac gttgatgatg gcctgcgctc gggtttgatc acgctggacc aactgcagat
     6541 ggtggagatc ttcgcgacgc atcattcgcg tgtgcgtcgg cgctatccga atctggacaa
     6601 gcgccgcgcc attgccgaga ccgtgcgcgc catgatcaac acgttggtgc tggacctgac
     6661 cgagaccacg ctggccaaca tcaggcaggt ggcgcccgcc agcgccgacg atgtacgccg
     6721 cgcggcgccg ctggcggcgt tctccgtgtc ggtgcggcgc caggccgatg cgctcaagca
     6781 gttcctgctg gacaacctgt atcgccatta ccaggtgata cgcatgacgt ccaaggctcg
     6841 gcagatcgtg cgtgaattgt ttgtcgcttt catgaatgat ccgcgtctgt tggccgagga
     6901 tcatcggcgt cgggacgacg tcgagcaggc gcgggccatc gctgattaca ttgctggcat
     6961 gactgaccgt tatgccattc gcgagcatca gcagttgttt cagatgcgtt gacgtgaaac
     7021 gattcgacgc tgtgggtatt tgaaggcgct gtctctcggg gctcggggcg gggccggcac
     7081 ggcgtgcttc atccggatct gtctcggcca ggcgggcgcc gggctaaact cgcgttgaga
     7141 tcagccgaag gctgatctca accctcagac accgcccgac gactgccccc gcctggccga
     7201 gccagatccg gcgcacgcct tacgccggtc ccgccccgag ccccgagaga cagctcacgg
     7261 tggtggcatg ccggggggct accagcccgt cccccttcac atatatatca tgttgcaccg
     7321 tgggcattcc gatcgaaagc tcgccagggt tcctcggagc aggtcgagcg gcgagcaggc
     7381 gcagtggatt gtccgaggac ccctggatct cgttcagatc ccccgggcgc cgcgtgcgac
     7441 ggcgcgtggc gctgccatct gcggactgct tgatcgcccg atgagccagg cgctgagggc
     7501 cgacaccacg ccggcgaaga tcgtgtaggc cacgatgagc cagggttgtc cgccgttgag
     7561 cttgagcagg gcggtggcga tgaggggtgt gatgccggag gcgaagatgc ccgagaactg
     7621 gtagacgaac gagatgcctg tgtagcgcac cttggtgtcg aacagctcgc agaacagggc
     7681 ggcctcgggg ccgtagatgg ccgcgtagaa gatgccgaac gggatcacga tggacagcca
     7741 gatcagcagg atattgccgg agtgattggt catcaaccag aacgccggcc aggctgatgc
     7801 tgcagtgatc agcgaccccc agaaatacgt gcgcgtgcgg ccgaccttgt ccgacaatgc
     7861 gccgaagaaa ggaatgaaga aacacatgac gacggcggcg gccatcacac cggtcagggc
     7921 gtcggtgcgg ttgatcttga tggtctgggt caggtagttg atggagaaga ccgcgaagat
     7981 gttgaagaac acgccgtcga tgtatcgtgc gcccatgcct ttgaggatgt tgccgggata
     8041 acgcttcgcc atgtccacga acgggatttg cagctccgcg ttttcgcgtt tgacctgttc
     8101 gaactccggc gtttccttga tcgtcaagcg gatgtacaag cccacgaaca ccagcgccgc
     8161 ggaaagcatg aagcccacgc gccagcccca cgacatgaat gccgcgttgc tcagcgtgcc
     8221 tgacagcagg gcgaccacgc cggcggccag gcacagtccg atggacaggc cgatctgtgg
     8281 caacgacgca tacaacccgc gcttgtgctc aggggcgtat tcgtacgtca tcagcacggc
     8341 gccgccccat tcgccgccca gtcccagccc ttgcaccacg cggcacagca acaacaggat
     8401 gggcgcccag atgccgatcg aaccataggt gggaatgagc gcgatcagga aggtagccac
     8461 gcccatgagc accagcgtga agatcagcgt gctcttgcgc cctatgcggt caccgaagtg
     8521 gccgaaaaca acgccgccga acggccgcgc cacgaacccg acggcgaacg tcgcatacgc
     8581 cagcagggtg ccgacgaccg ggtcgtgact ggggaagtac tgcttgttga agaccaggcc
     8641 ggcgatgacg ccgtacagga aaaaatcgta ccactcgatc gtggcgccga tcagggaaga
     8701 agcgacaacc ttccgtatgg aagacgaagg cgcgtgtgat tggctcatga tgtgtttgtc
     8761 tcctggtttg ttatgggccg gcaatccggc tttgtctttt tgatggaccg ctctttccgg
     8821 cggctgcttg tttcaatccc gtggattcca gcttcttgtg gcctgtgagt gatcaggcgg
     8881 cctgcgcgtc ctccaggatg aagtccgatg cgcgctcggc gatcatgacc accggcacgt
     8941 tggtgttgcc ggagaccagc gtgggcatga ccgaggcgtc gacgacgcgc agcccttcca
     9001 cgccatgcac acgcaggcgg tggtcgacga ccgcgccggg gtcggatgcc gatcccatct
     9061 tggccgtgcc ggcggggtga aagatggtgg cgccgtactc gcggcagaaa tgcaggatct
     9121 cgtcgtcggt gcgcacccgg tcgcccggtc gatactcgcg cttcatcaac tggcgcagcg
     9181 gcgcggcgcc agccaggcgc cgggcatatt tgaagcccgc cacggcggtg cgctggtcca
     9241 gttcgctgga cagatagttc ggctggatgg agggcgcttc cagcggatcg gccgagcgga
     9301 tgttgatctg gccccgcgat tcgggccgca actggcacac cgagtaggtg catcccgaaa
     9361 aatcatgcac ggcgccgccg gccatatcgg ccgacaacgt gccgaagtgg aattggctgt
     9421 cgggcgtggg gctttcctcg ggcagcgccc ggcagaacac cgccccctgg ttgatgccga
     9481 tggccagcgg tccgccccgg aacaacagcc attgcaggcc gatacgcgcc ttgctccata
     9541 tcgtgcgcaa ttgatcattg gtggtgatgg gttgggtcac ctcgtagatc aggcggatct
     9601 gcagatggtc ctgcaggttc tgaccgaccc ccgacagatt cttcacgacc gggatgccga
     9661 accgcccgag cagatcggcc gggccgatgc ccgagagttg aagcaactgg ggcgactgga
     9721 tcgcgccagc tgacaggatg acttccttgt tggcaagcag cacgtgctcc tgcccgttct
     9781 tgcggtagcg cacacctgcc gcgcgggtgc cctccatcac caccgccgtg acctgggcgt
     9841 tcgtttccac gcgcaggttg gggcggttgc gggcggggac caggtacgcc accgccgtgg
     9901 agcagcgctt gccattgcgg gtggtcaact gatagtagcc gaccccctcc tgattgccgt
     9961 cgttgaaatc gcgccgcttc ttcaggccca gcgtcgcgcc ggactggatc agggcctcga
    10021 cgagtggatg cgacgtcttg atggacgtgg cgttcagtgg cccgtcggtg ccatgcgtgg
    10081 ggcccgcacc cagatcgttg ttttcgagct tgcggaaata aggcaggcat ttttcccagc
    10141 tccagcccgg gttgccggct tgctcccagt gatcgtagtc ctgcttttgc ccgcggatgt
    10201 agatgaggcc attgatcgag ctcgacccgc caagtgtccg gccacgcggc caatagatgc
    10261 ggcgccccag catgttgtcg tccgggtcgg tctggaagcc ccagttcagg gtcttgtgga
    10321 acatggtttt cccatagccg atggggatat gtatccacgg attgcggtcc ggcgggccgg
    10381 cctcgatcag gcaaaccgaa tacttgccgt tggcgctgag tcggttggcc aggacgcagc
    10441 cggcggagcc ggctccgatc acgatgaaat cgacggtatt gggcatggta atgttgattt
    10501 ggttgtctca ccccgtcggg cacccggagg cgcctcggcg gtttgtttat ataattatcg
    10561 gaacatgcgg tttcataata tttattcttc tgcgcgccgc aagcactaat cgtctgtcgt
    10621 tccgtgagtg aaacactaag ttccgctcgc gtttcatttt tgacgccgcg atgccgtttt
    10681 ttgtcagagt ctgttcacca cctcggggtt tcagtcaacc ttgaagggcg gtctttcaaa
    10741 ttagaaggct ggtgccatgt cccaccctat gatccgttat ctgcgcattc ccgctttgct
    10801 ggagcaactg gcccgcgccc gccacccgca gacgctggcg caactggcgc agcgcctgga
    10861 cctgcccaag acgtcgctga tgcggatcct ctcatcgctg gtggagtcgg gattcgtggt
    10921 gcatgcgccg ggggaacggg gtttcgtact ggggccgcgc gccacgcgcc tggctctgga
    10981 ggtgctgggc accccacact tttcccggtc ctcgcgggcg atcctggcgc gcctggtcga
    11041 ggccaccggt gaaacctgca acctgacagc gctcgagggc gatggcatgc gctatctgga
    11101 gcgcgtcgag accgggcacc tgctgcgctt gaaactggac gtcggcacgc acgtgccgct
    11161 gcattgcacc gccagcggca agctgatgct ggcgatgatg gacgacgcgc agcggcgcca
    11221 ggttctggac cgcatcgccc tggatcgcca cacgcctaaa accctcaccg atcgtgtgct
    11281 gctggagcgc gaactggcga cgatccggca acggcgcgtg ggggttgatc aggaagagtt
    11341 cgtgctgggc atggtggcgg tgtccgtgcc ggtgctgaat gccacgggcg gcatgatcgc
    11401 cgccgtggcc tgccatggac ccaccgcccg gatttccatg gcgttcctgc tggaatcagt
    11461 gccagtgatg cgggaagcgg cgcttgccat gggcaaggtg ctgggggatg acgcaatggt
    11521 ctggtgaggc gccaccgaga gtgtcccgca acaatacggt tcgctccctc ggcattggcg
    11581 cgagaaccgc gcaagcggag tatcccccac tatctggggt tgtgtggcgt gaccagcagc
    11641 ggcttgagat agcggccggt gtggctggcc gcgcaggcgg cgatgtcttc gggggtgccc
    11701 tgcgcgacca gggtgccgcc cgcatcgccc ccgtcggggc ccaggtcgac gacccagtcg
    11761 gcggtcttga tgatgtcgag gttgtgctcg atgatgacca ccgtgttgcc ggcgtccacc
    11821 aactggcgca gtacgtccaa cagctgtgcg atgtcctgga aatgcaggcc ggtggtgggt
    11881 tcgtccagga tgtacagcgt gcggccggtg ctgcgcttgg acagttcctg cgacaacttc
    11941 acgcgctgcg cctcaccgcc tgacagcgtc gtggcgttct gccccagccg gatgtacgac
    12001 aggcccacat ccatcagcgt gttgagcttg cgggcgatgg cgggcaccgc gctgaaatgc
    12061 gcgagcgcct gctcaacggt cagttccagc acatcgctga tgttgtggcc atggtagcgg
    12121 atgtccaggg tttcccggtt gtagcgccgg ccgttgcaga catcgcaggg cacgtacatg
    12181 tcgggcagga aatgcatctc caccttcacc acgccgtcgc cctggcaggc ttcgcagcgg
    12241 ccgcccttca cgttgaacga gaagcgcccc ggcccgtaac cccgggtgcg cgcctccggc
    12301 acgccggcga acagttcgcg tatgggcgtg aacatgccgg tgtaggtggc ggggttgctg
    12361 cgcggcgtgc ggccgatggg gctctggtcg atggtgatga tgttgtcgaa gtgcgaaagg
    12421 ccgtcgatcg aatcgtaggg cgccggctcg gtctgggccc ggtgcaggcg ctgcgccacg
    12481 gcggtcgcca gggtgtcgtt gatgagcgtc gatttgcccg agcccgaaac gccggtcacg
    12541 cagaccagcc gcccggcggg gatggccaga tccacggatt tcaggttgtt gccgcggcag
    12601 ccctttattt ccagccgggg caggtcggcg gtgactgggc gccgcggcgg gatggcaatg
    12661 cggcgggcgc cgctcaggta ctggccggtc agcgattgtt ccatgcgcgc gagatcgtcg
    12721 ggcgtgccgt gcgccacgat ttcgccgccg tgctcgcccg cgccggggcc catgtccacg
    12781 acgaaatcgg ccgaacggat catgtcctcg tcgtgctcga ccacgatgac gctgttgccc
    12841 aggtcgcgca gatgcttgag tgtctcgatg agacggtcgt tgtcgcgctg gtgcaggccg
    12901 attgacggct cgtccagcac gtacatgacg ccggtcaggc ccgagccgat ctggctggcc
    12961 aggcggatgc gctgcgcctc gccgcctgag atggtgtcgg cgctgcggtc caggctcagg
    13021 tagctcagcc cgacgttgtt caggaattcc agccgcgccg cgatctcgcg cacgatgcgc
    13081 tcggcgatat cgcgcttgcc gccttccagc tccagggcct cgaaccaacc cagacaggcc
    13141 gacagcggca acgcttcgac ctcgaagatg gcacgcccgc gccgctctcc cggcccatcg
    13201 tcgttgccga tcaacacgtt gcgcgcctcg gcgcgcaggc gtgagccctc gcaggccggg
    13261 caaaccttgg tgtggcgcag cttgcccagt tcttcgcgca cggtgctgga gtcggtctct
    13321 ttccaacggc gctcgagatt gggaatgacg ccttcgaacg gatgcttgcg cgtgaccgga
    13381 cggcctttct cggtcatgta gagaaagctg atttcctctt tgcccgagcc atacagcaca
    13441 cgctgcctgg cgtcttcgga aagatcctgg aaaggggtct cgagatcgaa gccataatgc
    13501 gcggccaggc tgcccagcac ggtatagctg aacgtgttgc ggcgatccca gccgctgatg
    13561 gcgccactgg ccaggctgag ctcggggaac gccaccaccc gcttggggtc gaagaaatcg
    13621 acgtggccca gtccgtcgca gtccggacac gcgccgaccg gattgttgaa actgaacaga
    13681 cgcggctcga gctcggtcag gctgtgcccg cagattgggc aggcataatg gctggagaag
    13741 gtctgctcct cactggaatc catgttcagc acggtggtgc ggccatccgc caggccggcg
    13801 gcggtttcca ggctttcagc cagacgctgc cggctggcgg gccggttgcg caggcggtcg
    13861 acgacgacat cgatatcgtg cttctggttc ttttgcagcg gcggcaggcg gtcgagttcc
    13921 accatgtcgc cattcacgcg caggcgcacg aacccttgcg cctgcagccg ggcgcattcc
    13981 tcttcgaatg tgcctttgcg gtcgcgcgcg agcggcgcca gcaccgccag gcgcgtatcg
    14041 ggcgcccagg ccagaacctt gtcgaccatc tggctgatgc tttgcgcttg cagcggcagg
    14101 ccatgttcgg ggcagtatgg cgtgcccacg cgggcgtaca gcaggcgcag gtagtcgtgg
    14161 atctcggtga tggtgcccac ggtggagcgc gggttgtgcc cggccgcctt ctgctcgatg
    14221 gaaatggccg gtgacaggcc ttcgatgagg tccacgtcgg gcttgtccat cagctgcagg
    14281 aactgccggg cataggccga caggctttcc acgtaacggc gctgcccttc cgcgtacaag
    14341 gtgtcgaacg cgagcgacga cttccccgac cccgacagcc ccgtcacgac cacgagctgg
    14401 tggcggggca ggtcgatgga gatgttcttc aggttgtgcg tgcgggcacc ccgaattcga
    14461 atcgcctctt tgggtttcgc gtgcattgtg ggtgtggtcg ggttccggta ttcggttgtt
    14521 cgtctggttt cgcagatttt ggccgacgat ttcgtcagat ggggtcgagt tgtcgcgccc
    14581 gatctgtcga gctggtcgtg ggcatgggct ttcaggtagt tcaaaggcca acttgctact
    14641 atagcgcgtg gcgcggatcc ttgcgcctta tggcctagcc ggcttcctga cgtttcgcct
    14701 cgttgattca ccactcgatc tcccgaatgc gcgaatctgg gcggggcgcc ggcgggcagg
    14761 gccgtctttt ctcctgttgg ctgtcacctg gacttcccat gacgaatcac actgccggag
    14821 agggccactc ccggcgcacc cgtctcaaac tcaaccctgt cgagcgccgc gccagcgtca
    14881 ccctggcctt gctgttcgcg gcccgcatgc tgggcctgtt cttgctgacc ccggtgttcg
    14941 ccgtggcggc gctgtccatt cccggcggcc atgacgccac caaagtgggc ctggccctgg
    15001 gtgcctacgg cctgacgcag gcgatcctgc aggtgccgct gggcatggcc tccgaccgct
    15061 atggccgccg gccaatcatc gtcgcaggcc tgctgctgtt catcgtgggg ggcgtcatct
    15121 gcgcgctcgc gcccaacatc gactggatca tcgtcgggcg tgtcgtccag ggcttgggcg
    15181 ccgtgtccgc cgccatcacc gcctgggtcg ccgacgccac ccggcccgag gtgcggacac
    15241 gcgccatggc catggtgggc ggctccatcg gcattgcttt tgccgtgtca ctggtcctgt
    15301 ccccgctgct ggtgggggcg ttcggcctgt caggcctgtt ctgggccatc agcctgctgg
    15361 gcttcgtgtg cctgctgatc gccaccttcg tcgtgcccgt cgtcacgcca agcgaaacgc
    15421 ccatcgtgca ggccagcgcg cgcgacgtgc tgcaccacgg cgacctgctg cgcctgaact
    15481 tcggtgtgta ttgcctgcat ttcacgctga tgtcgatctt catggtggcg cccggcctgt
    15541 tgcagcacct tggcggctac accgaatcgc atctgtggga agtctacttg ccggtcatcc
    15601 tggtgtcgtt cgtgctgatg gtgcccgccg tgttctatac cgaaacacga catgcccaca
    15661 ggctggcgct gcaggtatcg gtggccggac tggtcgtggt gctggggctg ctgcccttgg
    15721 ccagccacgg gttctacacc ctggttatcc tgatggtggg ctttttcatt gccttcaaca
    15781 tcctggaagc cctgcagccg tccctggttt cccgggtggc gccacccgag ttcaaggggc
    15841 tggccctggg gttctacaat accgctcagg cgctcggggt gttttccggt ggcgtggtgg
    15901 gcggcgtgct cgcccagcac gccgggacat cggaggtgtt cctggtggcc gcggttctgt
    15961 tgttcgcgtg gttcctggtt gcccgcgggt tcaagtacgg gggctgagga aaggcgttga
    16021 ggcccttcgg gttgccggca aggcagggca accggtacga tggcgctttg gccgttttgt
    16081 ggtggcacgc ccgcaggcgg tgccaccatg ctccgggtgc atcccgcccg gttgtttcca
    16141 ggccgggaca atatcggcca atcgtacatc acgaaaatca gcccagcagt ctctggtttg
    16201 ggccgataat catcatcatc tgacattcca agcaagggga taccaccatg gcctcagtca
    16261 acaaagtcat actcgtcggc aacctcgggc gagatccgga agtccgctac agccccgacg
    16321 gcgcggccat ttgcaacgtg tccatcgcca ccacctcgca gtggaaggac aaggcctcgg
    16381 gcgaaaagcg cgaagagacc gaatggcatc gcgtcgtgtt ttacaaccgc ctggccgaaa
    16441 tcgcgggtga atacctgaag aaaggtcgtt ccgtgtacat cgagggtcgc ttgaaaaccc
    16501 gcaagtggca agacaaggaa accggcgccg accgtttcag caccgacatc gtggccgacc
    16561 agatgcaaat gctgggtggg cgcggtgagg gcggtgattt cggcggaacg gaatacggtg
    16621 gcgcggcacc acagccgcgc caacagtcgc agcagcgcgc cgcagcaccg cgcccggcgg
    16681 ctgcgccggc cacgaatctg gctgatatgg atgacgatat tccgttctag gcaacactag
    16741 tttttagtgt ttataaaaag tagtgcaaag ccccggcatt tctttaatgt cggggctttt
    16801 tgcttgtatt tgaccgtgga aaatggatca ctatggtagt ggcgcttatg taaaaacagg
    16861 cgggaaaggg cgattgatgc ctagactgac aggtcaccac cgccgctgtg gcgtcccgcc
    16921 tgcttgatcg ggtcggcgtg ttcaccagca gcggcacgct gttcttcttg cgaacctctc
    16981 tataaggcgg ccccatgaac cgcttatcac acggcctccc gcttgacgcg gcccgtcccg
    17041 cctcgcctgg cgaccattag caccccgacg gccagcgcga gcggcacgaa cgatacccat
    17101 agcaccatgt cccagccaaa agcggtcagc agggcgccgg aggcaagcga gcctatggtc
    17161 atcaggccga agacgatgaa gtcgttgaat gactgcacgc gtgttttctc ttccggtcgg
    17221 tggcattcca ggaccagcgc ggaggcgccg agaaacccga aattccagcc catgcccagc
    17281 agaatcagcg ccagccagaa gtgggtgaca tcggtcccgc ccaggccggc caccgcagcc
    17341 aggccgatga gcgccatgcc ggtcagggag acacggccgg cgccgaaacg cgtgatgagg
    17401 ccgccggtga agaaactggg ggcgtacatg gccacgacgt gccactgcag gccgttgttg
    17461 gccgaggttt gtgaatggcc ggtcatgtgc atggcgagcg gcgccgccgt catgaggaaa
    17521 ttcatcagca ggtaggacac ggcgccgcaa acagcggccg caatgaaccg gggttgccgc
    17581 acgatttcga tcaacggccg gccagcggcg atctcggtgc gggtcggcgt gggcaggcgc
    17641 acgccaagca acacgaaagc cgaaatggcc gccaccgcgg cctgtatgaa aaacgtggcg
    17701 gtgaatcgat gtggcggcca cagatccatg gtccaggtca cgagttgcgg cccgaacacc
    17761 ccggcggcga caccgcccgc catgaccaat gacaaggcac gcgcgcgctt cccgggcgcc
    17821 acgccatcgg cggcggcgaa tcggaaagtg agcaccacgg cggcgtaggc gccgcccagg
    17881 agggtcgcca ggcagaacag ccaaaaccac cccatgacga ccgcgagcgc agcgaacagg
    17941 ccggccagga ccccggtgga agttccggcc aggaatgcgg tgcgtcgacc gtggcgccgg
    18001 gcgatggcgc ccaccggcag ggtgcaggcg gccatcccga tgacgaaaat cgaaatgggc
    18061 aacgttgcca gcatgggcgt cggggcgagc tggtcgccga cgatggcccc ggtggcatag
    18121 gtcaccaccg aattggcacc cgccaaggcc tgggcgatgg aaagccgcca gatattggcg
    18181 cgctgcgctg cgtcggataa cggggctggg cggttgatgg cggcggtgtt gtctggataa
    18241 gtcatgtgag ggatcggtta cttgacgcgg gcaatcccgc agagactggc aataaagacc
    18301 agcagccacg ggatggtcag cgtatcggga ttgaaagcgc ccgccagggc cctatcgcgc
    18361 cgcgtcgccc atacggtagt cgtgcgtcag ttcatcaaga tgtgccttca ggttgttgtc
    18421 cagcgtcgtc tcagccgccg cgatcacgtc cttcaactgt tcaggccggc tggcgccgat
    18481 gatggccgag gtgacggctg ggttggccag cacccaggcg acggcggcgg tggtgagcga
    18541 cagcccgctg tcgcgcacgg catcgaccaa ctggctgacg gtggcgaact cccgttcgtg
    18601 ccagtaccgc ttctggtaca tgcctccggc cgtacccagg gtgaaccgcc cgccctcggg
    18661 cttggtgtcc cgatgatgct tgccggtgag cagcccgccc gccagcgggt tgtagctgat
    18721 cacgccgagc ttctcttcgg cgcacagcgg cagcagttcc cgttcgatct ggcggaacag
    18781 caggttgtag cggggctgca cacagacgag gcgggcgagg ttgcgcgcat ccgaccgccc
    18841 cagagccttg gccaggcggt aggccaggaa attcgacacg ccgatataac gcaccttgcc
    18901 gctgcgcacg atggcatcga gagcttccag tgtttcgtcc agcggggtgg tctgatcgtc
    18961 ggaatgcagt tgatagagat cgacgtaatc cgtgtcgagg cggcgcaacg aggcatcgat
    19021 cgcgctgatc aggtgcttgc gtgacgcgcc caggtcccat tcgtgcgggc ccatcctgcc
    19081 gacggccttg gtggccagca cgaaatcgtc gcgtcgaccc ttcagccagc ggcccacgat
    19141 ttcctcggtt cgccccgctg tatgaaggtc accccccagc ggatacacgt cggccgtgtc
    19201 gatgaacgtg atgccctgcg cggcgctttc atccaggatg gcccgggcgg cggcctcctc
    19261 gatctgtagc ccgaaggtca tggtgcccag acaaagacgg gacacggtca ggccggtgga
    19321 tccgagcttg gtgtattgca tggatggctc ctggggagtg gggcggtttt catggtggtc
    19381 aattcgtgca gaataccgca ggcagacatg gattgctcct gccggcaatg ttcgcgaagc
    19441 gtgtggagtt gtcgtttgag cgcaagcagt tcgttgatgc gtgtctcgac gtccgcgata
    19501 tgctcgtcca caagctggtt cacgcc
//
LOCUS       DASTNK010000142        59495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1226465, whole genome shotgun sequence.
ACCESSION   DASTNK010000142 DASTNK010000000
VERSION     DASTNK010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 59495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59495
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226465"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1350)
                     /gene="ugpB"
                     /locus_tag="VFR20_10560"
     CDS             complement(37..1350)
                     /gene="ugpB"
                     /locus_tag="VFR20_10560"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HEU0231004.1"
                     /translation="MRAKHFALSLSLAGMIAAMGTASAATTITFWHAMQGPLGQRVDQ
                     MVDEFNKSQNEYVVKAIYKGNYNEAMNAGIAAFRAGNAPDILQVFEVGTATMMYAKGA
                     IEPVQEMSDKAGNPINPKDFLGAVASYYSSPNGKLVSMPFNSSTPVFYYNKDAFKKAG
                     LDPNKPPRTWEGVEADAKKLRAAGMECGYTSAWPAWVLLENFSAWHNVPYASENNGFG
                     GLGARLEINKPIYVRFMTFLQKMSKEGTFTYGGRGDAANALFISGKCGMYTGSSGTRA
                     NIVKNGNFSFGTSELPYLTGVKGAPQNSIIGGASLWVFAKKSPAVYKGVTAFFKFISS
                     PKQAAAWHQDTGYVPVTKAGYELTKKSGFYEKNPGTEVAVKQLDANTTKNSRGIRLGY
                     LPQIRDIENGDMENIFAGKISPKAGLDDMVKRGDELLVRFQKAVK"
     gene            1634..2461
                     /gene="proC"
                     /locus_tag="VFR20_10565"
     CDS             1634..2461
                     /gene="proC"
                     /locus_tag="VFR20_10565"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU0231005.1"
                     /translation="MTKELKLAFIGGGNMASALASGLIGKRCGASDVHVIDPYAPTLE
                     GWARRGVSTAPAAGPELGRHDVWIFAVKPQAMREAVNACRPFLRPGTLVVSIAAGVRA
                     DTMAEWLGGDHPHTRLVRCMPNTPALIGEGVSGLMALSGVSTEDKTLAEQLLRAVGEV
                     VWVDSDAMIDAVTALSGSGPAYVFLLLEAMIAAAVSQGLAADQARTLALGTLRGATQL
                     AALSPETPAQLRERVTSKGGTTAAALQVFRQEGFESIVARAMAAARDRAAAMAAEFH"
     gene            3019..3948
                     /locus_tag="VFR20_10570"
     CDS             3019..3948
                     /locus_tag="VFR20_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249233.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HEU0231006.1"
                     /translation="MSDLLPQLTQQFFNGLSLGAIYALIAIGYTMVYGIIGMINFAHG
                     DIYMIGAYVGLVTLSAIGANSGLPLPVVVLIMILVAVAVTGVYGYATEKLAYLPVRHS
                     PRLVPLISAIGMSIFLENWVAIGQGARDLAVPQLIKGSFQFTLGASTFQVTASYSRIM
                     IIVVTVLLMIGLSLFIKHSRTGRASRACSQDLHMANLLGIDTNRIISFTFVLGAMLAA
                     VGGVLIALAIGKLNPFIGFIAGIKAFTAAVLGGIGSIPGAMLGGVLLGLAETFAAAYI
                     SSQYKDIVAFGLLVFILLFRPTGLLGKPEVEKV"
     gene            3948..5177
                     /locus_tag="VFR20_10575"
     CDS             3948..5177
                     /locus_tag="VFR20_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HEU0231007.1"
                     /translation="MVKSNFTNALIAALMSGILIAPVFGFRIITHGMQSDLQPQWPMI
                     VMGMVIVFVAQLMRPAIKSSMSRRMSLPSLPAITDSVRRKGLVLLILIALVWPFFGNR
                     AQVDLATLVLIYIMLGLGLNIVVGFAGLLDLGFVGFYAVGAYTYALLYHWAGWSFWEA
                     LPLSGAVAALFGFVLGFPVLRLRGDYLAIVTLGFGEIIRLMLINLYGLTGGPDGISGI
                     PKPTVFGYPMLRRAPEGVVTFHQLMGWSFNNQDIIIYMYLATLLLVFFALFVSTRVIR
                     MPIGRAWEALREDEIACRSLGLNPTRIKLSAFTMGATFAGLGGAFFAARQGLVNPESF
                     TFIESALVLSIVVLGGMGSQLGVILAAILITVVPELAREFAQYRMLVFGLVMILMMMW
                     RPQGLLPVKRPQVELAS"
     gene            5174..5941
                     /gene="livG"
                     /locus_tag="VFR20_10580"
     CDS             5174..5941
                     /gene="livG"
                     /locus_tag="VFR20_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HEU0231008.1"
                     /translation="MNDTLLSVSGLTMRFGGLLAVDAVEFDVRRDEVFAIIGPNGAGK
                     TTVFNCVGGFYKPTTGTITMDGTPINGLSSHKVARKGLVRTFQNVRLFKSLTVLENLL
                     VAQHMHIQTGLLQGLLKVSSYRKSERDALDRAVEWLEFMGIRALANRDAGTLAYGHQR
                     RLEIARCMITRPRLLMLDEPAAGLNPQEKRDLQTLIDRLRREFGVAVLLIEHDMSLVM
                     GVSDRIMVMEHGRPIITGGPAEVRADPRVIKAYLGEE"
     gene            5942..6643
                     /locus_tag="VFR20_10585"
     CDS             5942..6643
                     /locus_tag="VFR20_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0231009.1"
                     /translation="MLKLENVHTHYGAIQALNDVSIEVNQGEIVTLIGANGAGKTTLL
                     MTVCGTPQASAGRVFFEERDITADSTHMIMRRGIAISPEGRRVFPDLTVSENLKMGGF
                     FLSKMEIHAGLEHVYGLFPRLRERANQRAGTMSGGEQQMLAIGRALMTRPKLLLLDEP
                     TLGLAPLIIAQIFDIIRTIREQGVTVFLVEQNANKALQVADRGYVLETGRVVLADSGA
                     NLLVNDDVRKAYLGG"
     gene            complement(6668..7276)
                     /locus_tag="VFR20_10590"
     CDS             complement(6668..7276)
                     /locus_tag="VFR20_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0231010.1"
                     /translation="MKLYYLPGACPLAAHIVLEWTGAPYELQQVSRTEIKEPAYLKMN
                     PLGAVPVLQNDGITLTQNAAILEYLSELHPGADLVGKTKEERAETRRWLGFCNSDLHR
                     TFSLVFSAAAYVSDPKVQEELVRNVMNRLHTLFGILNDQLKGKTWLTGKRSIADPYLY
                     TVMRWAKGKQVDLAGMADLEAFFQHMSADTGVQAALKAEGLS"
     gene            complement(7463..9340)
                     /locus_tag="VFR20_10595"
     CDS             complement(7463..9340)
                     /locus_tag="VFR20_10595"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU0231011.1"
                     /translation="MIEASEGAGALPARYPIRDFFKNPQRGSFRLSDDGATLGFMEPV
                     SIDGKPPRMNVFIQTLAGSTPTGASRRLTAETERDVSGFFWKGNDTILYEKDFNGDEN
                     FHVVAVDTASGAITDLTPFPGVRAGVQDDLEDDPDHILISHNKRRAEVFDVYRANLRT
                     GELALVAENPGNIVGWQADHEGRIRAAIASDGLNTTVLYRNDESQPFRPIIHTDFRTS
                     VGPLFFTFDNRSLYALSNRGRDCLALVVIDPERPDDERLVYQTPEVDLDGASYSRLRK
                     VLTEASFNTDRPHHHFFDDVAARRHEDLRARLPGYEVALQSASRDEAKFIVAAYNDRT
                     PGARYLYDAENRTLDKLADINPALQEADMAPVRPVTYTARDGLTIHGYLTLPPGRPAR
                     ALPCVINPHGGPWARDHWGFNPEAQFLANRGYAVLQMNFRGSTGYGRKFWEASFGQWG
                     LAMQDDVTDGVRWLIDQGIADPARIAIYGGSYGGYATLSGIVGTPDLYAAAIDYVGVS
                     NLLTFMNTIPPYWKPMLEKMHCMVGDPVHDRDRLVATSPALNADRIRTPLFIAQGAHD
                     PRVNKAESDQMVAALKKRGVDVDYLVKDNEGHGFHNDENKFEFYERMEAFLDKHLGPR
                     G"
     gene            9568..10560
                     /locus_tag="VFR20_10600"
     CDS             9568..10560
                     /locus_tag="VFR20_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEU0231012.1"
                     /translation="MSEKFTRIVFLDRETISPQTVLRPASFPHDLVVHQHTAPGDVAA
                     RIADADIVITNKVRLGREALSGASRLRMIAVAATGTDVIDLPACADHGIVVSNIRGYA
                     VNTVPEHTFALIFALRRSIVAYRESVKAGRWQESGQFCYFDYPIRDLAGATLGIIGGG
                     VLGQAVATIGRALGMRVVFSARKGVAQADARYTPFEQVLAQSDVLTLHCPLNDETRGM
                     VGDKEFALMTRKPLLVNTARGGLVDEAALERALRSGRLGGAGFDVATPEPPSGDSVLM
                     RLLDLPNFILTPHVAWASDEAIQSLADQLMDNIDAFQSGTPRNVVAAPGAAPRK"
     gene            complement(10503..11357)
                     /locus_tag="VFR20_10605"
     CDS             complement(10503..11357)
                     /locus_tag="VFR20_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0231013.1"
                     /translation="MLLTRRHIAVSPRQRQALNPSGHLFAVAGLAALAFAMIENGTYG
                     WASMPIGCALILSAAAFTAFIARERHSATPIVPRTLRRNTRFTAANGIGFFINLAAFG
                     QLFLISLYLQQVRGDDAWRIGLDLLPLLMMFTLGNLASGKVGARWGTRMPLSLGLASA
                     ATLTAVVTATATILPYWLFAALIMLINLGVGIAIPAMTVTVMEVAGQDHANIAAAALN
                     ANRQIGALIGVALIGTILHSAPAWPRSLPVTFGLITMGYLVAAALALRYLRGAAPGAA
                     TTLRGVPD"
     gene            11489..12883
                     /locus_tag="VFR20_10610"
     CDS             11489..12883
                     /locus_tag="VFR20_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU0231014.1"
                     /translation="MEHWREALAAFRGGEAKYKILVQAITRDIENGSLPDGARLPPQR
                     DVGRELGISVQTVSNAYKELERDGLIRCEVGRGSFVSRNVTEQVSSYMLDHSERSIAD
                     FSTARIIHTLEHEHAWRDICAAMSREADQPWMRDCRPIAGFDRHRAAAVAWLAHMGVE
                     STPDTVLVTNGAAHAVFLALASLVGSDDTVACEKLTDHGVIGAAHVLGFTLKGLDIDE
                     YGIRPDHFEDVCANERISALVCIPNLHNPTGVVMPEARRRSLVRIAQRYGVYIVEDDV
                     YGPLLDTRPPPLSQLAPDLGFYCTSFTKSVMTGLRIGYLSVPTRFALRVNGILRVNSW
                     MATPLMAEVAARWQEDGTAEHLIQIQRERLAKRHAMVDEILGDYVAWHHPAGLSVWVN
                     VPDHWRTETLVGVLRRRGIAVTSPEPFSVRQSHRTKAFRLCVGADIDDDETRRALVEV
                     RSTFEQYPLAHDFV"
     gene            13019..14008
                     /gene="eutB"
                     /locus_tag="VFR20_10615"
     CDS             13019..14008
                     /gene="eutB"
                     /locus_tag="VFR20_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyectoine utilization dehydratase EutB"
                     /protein_id="HEU0231015.1"
                     /translation="MMALSLGDVYQARNKLAGRVCRTPLVHSPVLSQLAGTPVHLKME
                     SHQLTGSFKLRGAANAIGNLDEAQRKCGVVTASTGNHGRAVAYAARQRDMRAVVCLSS
                     LVPQNKIQAIRDLGAEVCIEGNSQDDALARADRFVTEDGLVMVPPFDHPDVIAGQGTV
                     GLEILEQLPETGSVLVPLSGGGLLSGIALAVKAARPAITVVGITMESGCAMHASLRAG
                     HPVQVDETPSLADSLGGGIGLDNRLTFSMVRDLCDRTLLTSEAAIAAAIVHMYRHERE
                     VVEGAAAVGVAALMDGLVGDLAVQGPMVLIVSGRNIDMARHLDLLARSVSPPY"
     gene            14027..15019
                     /locus_tag="VFR20_10620"
     CDS             14027..15019
                     /locus_tag="VFR20_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase"
                     /protein_id="HEU0231016.1"
                     /translation="MPKVVILSEADLRACVSLDAEAVDCVERAFHLLATAPVVMPPIL
                     RLDIAEANGEVDVKTAYLPGVDSFAIKVSPGFFDNPKLGLPSLNGMMMLLSARTGLLQ
                     SLLLDNGYLTAIRTAAAGAVAARWLARADASKVAIVGAGEQAGLQLRALTLVRPVSSA
                     TVWAPNPEKAASFALRMTSLLSLPVQACVDVDTALADADIAVTATPSTHPLIFEHHLR
                     PGLHITAMGSDAGHKNEISPTAVKAVDLYVCDRLSQARALGELRHAIARGAIDEDAPM
                     IELGQIIAGQSPGRRSDDDITLCDLTGTGAQDTAIATLAHARALRSGLGLDFQS"
     gene            15040..16269
                     /gene="doeA"
                     /locus_tag="VFR20_10625"
     CDS             15040..16269
                     /gene="doeA"
                     /locus_tag="VFR20_10625"
                     /EC_number="3.5.4.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049618.1"
                     /note="DoeA (degradation of ectoine A) is also called EutD
                     (ectoine utilization D); Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0042400 - ectoine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine hydrolase DoeA"
                     /protein_id="HEU0231017.1"
                     /translation="MDTLDIPLAVDEPGSSNAVELPFERGEYAQRLKKTREAMMARGI
                     ELLIVSDPTNMAWLTGYDGWSFYVHQCVLVALEGEPVWYGREQDAYGAQRTVYMAGDH
                     IASYPDYYVQSTERHPMDLLCQEVVARLGCLRCRIGVEMDNYYFSAAAYAALRRNLPD
                     AQWVDATGLVNWQRAVKSPREISYMRVAARIVERMHQRILEVIEPGMRKNELVAQIYA
                     AGIQGVPGHGGDYPAIVPLLPTGRDAAAPHLTWNDAPMAGNAGTFFEIAGCYKRYHCP
                     QSRTVYLGHPPAHFLEAEQAVLEGIDAGLEQARPGNTCEDVANAFFQRLKRYGIEKTS
                     RCGYAIGASYPPDWGERTMSLRPGDRTVLEPGMTFHFMPGLWQKDWGLEITESILITP
                     TGAETFCNTPRKLFIKE"
     gene            16274..17275
                     /gene="doeB"
                     /locus_tag="VFR20_10630"
     CDS             16274..17275
                     /gene="doeB"
                     /locus_tag="VFR20_10630"
                     /EC_number="3.5.1.125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006405502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042400 - ectoine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N(2)-acetyl-L-2,4-diaminobutanoate deacetylase
                     DoeB"
                     /protein_id="HEU0231018.1"
                     /translation="MRNSPIKPTVDFTREGVQHGFLKLPYSRDESAWGALMIPITVVR
                     RGIGPTVLLTGGNHGDEYEGPVALVKLANSLKASSVSGCVIIIPFLNYPAFLAGRRTS
                     PIDHGNLNRVFPGRPDGTVTEKIADYVERYLLPMADYVLDIHAGGKTLDFLPLAAIHI
                     LDDKQQQARCEAAMRAFGAPYSLKILELDGVGMLDSSAEQAGKVFVSTELGGGGAVTA
                     ASIAVADAGVHRFLVHAGVCRGKSPSRGTIMLDMPDDACYTQSEHDGLLEMCKSLGDH
                     VEAGDVLARVYDTTRTGVPGIAYRAGCDGILTARHFPGLVKCGDTIAVVARVVPNAD"
     gene            17308..17811
                     /locus_tag="VFR20_10635"
     CDS             17308..17811
                     /locus_tag="VFR20_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEU0231019.1"
                     /translation="MVKLDRYDLQILKTLATRGRITKSELAKIVCLSVSPTWERVKRL
                     ETMGLIRGYRAVVDWGSIFRSSQVIVEVRLDRHTAYDMQRFEQRVAEAPEVSQCYATG
                     GGVDYFLHVRARDIDQYQRFIDGLLLENLGIDRYFTYIVTKIIKTAQVIPAEAEEPVK
                     AAPNNSL"
     gene            18015..19511
                     /locus_tag="VFR20_10640"
     CDS             18015..19511
                     /locus_tag="VFR20_10640"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEU0231020.1"
                     /translation="MFLAQPLLFKSLCYINGHWVHSDSGASLAVHNPADQSVLGHVPM
                     LEKLQIEASVQAAQRAQASWRHVPVNERSAHLHRWAGLILDNRDDLAKIISLEQGKPF
                     SEALGEIDYAASFIPWYAEQARRLDGRFIPSHLTNAQPGTLKEPVGVAALITPWNFPS
                     AMITRKAAAALAAGCSVVVKPAHETPFSALALAQLAEQAGFQPGLFNVVLGEPQMVMQ
                     TLVSHPLVRAVSFTGSTRVGRLVMRAVAEAGVKRVALELGGNAPFIVTPDADLDLAVR
                     VAVEAKFQTSGQDCCAANRILVHRDLYAPFLDRYVKKVGNLRVGPGLDPASEIGPLIH
                     QAAFDATVQRVDDALRRGARLLTGGLPDAQGGLFFQPTVLADVAPGMRIYDEENFAPI
                     SGVSSFDTLEQVVELANDTPYGLAAYLCASRSDVIWPLLRKLDFAMVSVNGARFTGAP
                     VPFGGMKESGLGREGGDEGFDPFVETKYFCLSNLGLPATGTPHFRSTL"
     gene            19508..20890
                     /locus_tag="VFR20_10645"
     CDS             19508..20890
                     /locus_tag="VFR20_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU0231021.1"
                     /translation="MKKTAEQLLAIDRQYFMHPSTHAHDHESGALPGRIVTGAKGIRI
                     TDHHNETFIDGFAGLYCVNIGYGRTEVADAMYEQAKRLAYYHTYVGHSTNAIAELSGR
                     IISWAPKGMRKVYYGMSGSDANETQVKLVWYYNNILGRKNKKKIISRMGGYHGSSIAS
                     GSLTGLDAYHKNFDLPLDRIKHTVRPHWYREAPDGMDESAFAQYCADKLEELIQAEGP
                     DTVAAFIGEPVLGTGGIVPPPTGYWRAIQAVLKKHDILLIADEVVCGFGRLGTKMGSH
                     MFDIQPDLITIAKGLTSAYAPLSGVIVGERVWDVISRGSEEQGAMGHGWTYSGHPVCA
                     AAAVANLNILETENIMANAAEVGAYLLDSLHTTFDGHPMVGEVRGVGMLAALEFMAKP
                     KERVAFDAALKVGPQVAAACMSRHLIARAMPGYDVLGFAPPLVTTRQDVDEIVRITKE
                     AVDEVANQVM"
     gene            21028..22077
                     /locus_tag="VFR20_10650"
     CDS             21028..22077
                     /locus_tag="VFR20_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HEU0231022.1"
                     /translation="MSRPFRLAINGYGRIGRCFLRALYESPLRHKFQIVAINEPADLE
                     SMAHLTRFDSTHGVFPGKVACGDRALLIDDEPISVSHSSSPEGVDWGSLGIDMLVECS
                     GHFGTRADLERFLAAGCPRVLVSHPARSAADVDFTAVYGTNHEQLDPSSRLVSNASCT
                     TNAIVPVLDQLHRAFSVEQVFLTTLHSVMNDQPMIDGYHHSDLRRTRSAMQSIIPVST
                     GLAQGVERLLPQLAGRVRAKAIRVPILNVSAIDFMVNLGRDVTEDTLNAVMADAAQAH
                     PRLIAVSNGPHASVDFNHDARSAIIDASQTRCNGPRFANLLVWFDNEWGFANRMLDMA
                     GFWASRLRLLIKDTP"
     gene            22074..23471
                     /gene="fumC"
                     /locus_tag="VFR20_10655"
     CDS             22074..23471
                     /gene="fumC"
                     /locus_tag="VFR20_10655"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEU0231023.1"
                     /translation="MTSTRVETDSMGQIDVPADHYWGAQTQRSILNFPIGVARFKWQR
                     PVIAALGTLKKAAAQANAELGELPPDLARLIGLAADEVIEGRLDGEFPLVVFQTGSGT
                     QSNMNANEVISNRAIELAGGIMGSKSPVHPNDHVNRGQSSNDTFPTAMHIAVAQELAH
                     ALFPSVGALRKTLAAKSEKFQSVVKTGRTHLQDATPITVGQEISGWVAQLDFALDAVQ
                     AALPGIYDLAIGGTAVGTGLNAHPRFGEEAARHITDITGLPFRSSPNKFFSLSAHDAL
                     VNLSASLRTLAGALMKIANDVRWLASGPRCGIGELVIPDNEPGSSIMPGKVNPTQCEA
                     MTMVCVQVFGNDAAVAFAGSQGNFQLNVFKPVMVHNVLESIGLLSDACASFNDHCAVG
                     IEPNLERIAEHLEENLMLVTALNRHIGYDKAAFIAKRAHKEKSTLRAAALASGYLTAE
                     QYDAWIVPLDMTHPG"
     gene            24109..24327
                     /locus_tag="VFR20_10660"
     CDS             24109..24327
                     /locus_tag="VFR20_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231024.1"
                     /translation="MKTTQRIAYCTMILAMSASLSACSGMSHRGQSTAIGAGVGALGG
                     AILTGGSALGTVGGAAVGGVIGNIAGQR"
     gene            24946..28809
                     /locus_tag="VFR20_10665"
     CDS             24946..28809
                     /locus_tag="VFR20_10665"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231025.1"
                     /translation="MKLLSNGRYHVMLSAAGGGYSRHGQNALTRWREDATRDCWGAFL
                     YVRDRGNGDAWSPARQPMVDAAVERSIDFSGSSAAFIGTTSDITTRVDVTVSPDADLE
                     VRRVRVTNHSSRIRSLDLTSYAEVVLSSAPADDAHPAFNKLFVQTDIDESVSAILCHR
                     RPGNGGDPAPWLFHTVVVDSPGIANLSYETDRARFIGRGRTLADPAAGQVESLSGSQG
                     SVLDPVLSIRCPVRLEAGQSATVSFLYGIAENRAACEALIKGFQSQVKVDETFDDAAG
                     ARKKTLAKLNLNDSAAALYDRLAARVVYGGAQLRADPSIILANRQGQSGLWGFSVSGD
                     LPIVFLRVGASPDMGKVRELVQAHAYWRFMGLAADLVIANADGIEPRQGVQQQILDLV
                     KTLEQSDQLDQRGGIFVRLATRFSPEDRVLFLSVARVVLDDGPLGLEDRLIAVEGNTA
                     RPMHAKTPASIASMPARSPAAESSSPDRIIDNGIGGFTPDGREYVMTLDEGAMTPTPW
                     TNVLANPDFGTIVSESGCANTWSENAHEFRLTPWSNDPVCDSSGEAVYLRDETSGAYW
                     SPTPWPCRDQGAYVCRHGFGYSSFQHVHDGVESELIVYVSRSQPVKFTSLKVRNQSGR
                     RRRLSATGYVEWVLGDQRIKTMAQVTTRMNPSGRAIVAHNAYSMEFGDRHAFFSVDAD
                     PGSVTADRTEFLGCCGRAEQPVAMARSALMGAVGAALDPCAALRVPFELAPGEERDIT
                     FMLGAGGNEAATLGLVEQCGRAGAAQAALDEVQGYWRHTLDTVRVKTPDPAFNMQVNG
                     WLAYQALACRVWARSAFYQPGGAFGFRDQLQDVMALVHAEPSLVRAHILLSASRQYPE
                     GDVQHWWHPPAGRGVRTRCCDDYLWLALATCRYITVTGDAGILDEPVLFLDGRPLKPD
                     EDSYYELPGVSKEPAPLYEHCARAIRHGFRMGIHGFPLMGAGDWNDGMNKVGAQGKGE
                     SVWMAFFLYHILRQFKPLAQSRDDQEFVKECDAVAAGLADSLDKNGWDGEWFIRAYFD
                     DGSPLGSSKNTECRMGSIAQSWSVLSGAAAPERAHQAMDSLDRMLVDRDNAIIKLLSP
                     PFDVSVPDPGYIRAYVPGVRENGGQYTHAAVWAAMAFAELHDTRRAAELIAILNPVNH
                     ALDATAVERYKVEPYVVASDVYALPPHTGRGGWTWYSGSAGWLYRLLLESVVGVRREA
                     DKLFLRPCVPDHWDAFSVDYRYGATVYHIDVRREAKVAGSASPVIDADSLQSPVIPLV
                     DDGGEHRITFTLPASPNRDSAKVTH"
     gene            28805..29872
                     /gene="gnd"
                     /locus_tag="VFR20_10670"
     CDS             28805..29872
                     /gene="gnd"
                     /locus_tag="VFR20_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEU0231026.1"
                     /translation="MDYSRPQASETREIQTMQIGMVGLGRMGTNMAQRLMAKGVDCVV
                     FDTHQQAVSALSTEGAAGAASLRELVGKLAQPRAVWLMVPAGVVDGVLDQLVPLLSKD
                     DIVIDGGNSYYHDDIRRAGKLAEQGIHYLDVGTSGGVFGKERGYCLMIGGDDASVKHL
                     DPVFAALAPGRDTAPRTPGRNDGEGTAEQGYLHCGPHGAGHFVKMVHNGIEYGIMQAY
                     AEGLNVLCNANAGKRNRETDAETTPMRNPEYYQYDLNLPEVAEVWRRGSVISSWLLDL
                     TAHALHGNPDLGNFAGRVSDSGEGRWTIHAAIDEGVPVPVISAALFGRFQSRGNADFA
                     DKVLSAMRKEFGGHDEKNRGA"
     gene            29847..31283
                     /gene="zwf"
                     /locus_tag="VFR20_10675"
     CDS             29847..31283
                     /gene="zwf"
                     /locus_tag="VFR20_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU0231027.1"
                     /translation="MTKRIGGPDMVQPAKKVQPSDALVVFGITGDLAHKKIFPALYEM
                     VKRDGLNVPVVGVASSSMTLAQLRKRAADSIREAGKIDDKEALERLHGLLKYVQGNYN
                     DEATFHTLRHALGKARRVSYYLAIPPSLFGTVIQGLHHAGLAEDGRVIVEKPFGRDLA
                     SARELNRVAHSVFPEESIFRIDHFLGKEAIMNILYFRFANSFLEPIWNRDHVESVQIT
                     LSEQFGIGQRGGFYETAGCLRDVIQNHLFQIVALLAMEPPVHQDYAAIHTEKSKVFEA
                     MRPLGIDDVVRGQYTGYRKEPHVARTSDVETFCAVRLFIDSWRWAGVPWYLRSGKCMA
                     ETAAEVLVQLRPPPQRLFADSMSERQRSNYLRFRLSPNSAVALAARIKRSGKEFVGDQ
                     RELFLLDADPNQETPYERLLTDALAGDSSLFTSEDAVEAAWAVVDPILSRHHKAISYR
                     RGSWGPKEADALIAPHGAWLNPRLEERA"
     gene            31280..31993
                     /locus_tag="VFR20_10680"
     CDS             31280..31993
                     /locus_tag="VFR20_10680"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU0231028.1"
                     /translation="MTTPREETVFLFDVDNTLLDNDRVTDDLRRHLIEQFGRKSAERY
                     WALFEALRTELGYADYLGALQRYRLGLVNDPALLKMSSFLLEYPFAERLYPRVFDVLE
                     QAGKWGTTVILSDGDAVFQPRKVERSGLWDAVEGRVLIYIHKEKMIDAVMERYPASRY
                     VMVDDKLRILTAMKKRMGARLLTVFPRQGHYAHDLKTVRLFPPADITVEHIGDLLDVD
                     LPAHLRASDDEATTKEVTS"
     gene            31990..33090
                     /gene="tal"
                     /locus_tag="VFR20_10685"
     CDS             31990..33090
                     /gene="tal"
                     /locus_tag="VFR20_10685"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEU0231029.1"
                     /translation="MNATRQLHDLGQSLWLDNITRGLLDDGTLKHYLDDLAVTGLTSN
                     PTIFQKAIQGTTFYDEAIAHKAQAGKAGEDLFFELAIEDLTRAADLFRPVHDATRGMD
                     GWVSLEVSPLLANDTDATIHAATTLHARANRPNLFIKIPGTLAGLKAIEESIFNGVPI
                     NVTLLFSCEQYLAAAGAYLRGIERRVQAGLSSDVRSVASLFVSRWDVAVQSKVDAGLH
                     NRLGVAVACETFAAYRALLASPRWKKLEAAGARPQRLLWASTGTKDPSASDTLYIDGL
                     AAAGTINTMPEKTLLAFADHGNPGAPLRDEQPAAIAELAQFRAAGIDLGALATRLQEE
                     GTDAFDQSWHALLDGINAKSAALKSASTHSRT"
     gene            33095..34753
                     /gene="pgi"
                     /locus_tag="VFR20_10690"
     CDS             33095..34753
                     /gene="pgi"
                     /locus_tag="VFR20_10690"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU0231030.1"
                     /translation="MTPPSTPKLQPLTQSVGWKALTAHCSVAEGLHLRRLFAGDPERG
                     QRLALEAAGVYFDYSKQRVTDETIRLLLALARERGVEERRRAMFAGEAINATERRAVL
                     HVALRMPGTEQIRVDGHDVVPDVHEVLSRMGTFAQKVRDGKWLGYTGKPVRNIVNIGI
                     GGSSLGPVMAYQALKHYSPRDFTFHFVSNMDSTDLVEAVRDLDAEETLFIICSKTFGT
                     LETLTNAHAARQWCLDRLKDERALPRHFVAVSTNAERVAKFGIDTANMFGFWDWVGGR
                     YSMDSAIGLSTMIAIGPDGFSDLLSGFHAMDVHFRDTPLERNMPVLMGLLGVWNTNFL
                     GAETVAVLPYDQYLSRFPAYLQQLTMESNGKSVVTDGASQVNHQTGPIYWGEPGTDGQ
                     HSFYQLIHQGTHVIPCDFIGFCQGLNPLGDQHDLLMSNLFAQTEALAFGKTHEQVLAE
                     GVPAWQAPYRVFDGNRPTSTLLLERLTPHALGTLVALYEHSVFTQGVIWGIDSFDQWG
                     VELGKALALKVSPELTSPDEPALAHDSSTNTLIRRYRRLKAGRT"
     gene            34776..36854
                     /gene="tkt"
                     /locus_tag="VFR20_10695"
     CDS             34776..36854
                     /gene="tkt"
                     /locus_tag="VFR20_10695"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU0231031.1"
                     /translation="MAPNTELDQLCINTLRTLSIDAVQKAQSGHPGTPMGAAPIAYSL
                     WQGFLRYSPTHPDWLDRDRFVLSSGHACALLYSLLHLAGVRAHLPSYGDTDRLAVSLD
                     DLKRFRQLDSHCTGHPEYGWTTGVEVTTGPLGQGVANSVGMAIAGLWMAQTFNRPGFE
                     LFRHNVYALCGDGDMMEGISGEAASMAGHLGLSNLCWIYDDNHISIEGNTRLAFTEDV
                     GARFAAYGWRVVRVGDANDLDALNDAFQVFLSTTDRPTLIIVRSHIGYGAPHKQDTRE
                     AHGEPLGADEARLAKEFYGWDPDAQFHVPSEVPEHFARHLGQRGEAIRAAWQETYQAY
                     GKQYPDLARQIELLRNRALPEGWDAALPVFPADARGMATRASSGKVLNAIAGKIPWLI
                     GGAADLSPSTKTELTDPLYASFQAPAFDGNHGGRNFHFGIREHAMCAIANGIVVSGPR
                     PFASSFFVFTDYCRPSLRLSAMMGVPVIYIWTHDSISLGEDGPTHQPIEHLPSLRAMP
                     GMIVIRPADANEVVEAWRAIMPLRDHPVSLVLTRQSLPTMDRTRYAPAAGVARGAYVL
                     ADAPDGKPLVLLMASGSEVALCVQAYEQLREQGVGARVVSMPSWELFEAQDQAYRDQV
                     LPPSVTARVAVEEASGFGWERYTGLGGAILGMTSFGLSAPGGDVSRHFGFDCEHVVAA
                     AQAQMASRKS"
     gene            36982..38712
                     /gene="pgm"
                     /locus_tag="VFR20_10700"
     CDS             36982..38712
                     /gene="pgm"
                     /locus_tag="VFR20_10700"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HEU0231032.1"
                     /translation="MARAKKNESMKISPLAGKPAPRAILVDVPRLISSYYTGRPDPGV
                     TGQRVAFGTSGHRGSSLELTFNEAHVLAISQAVCDYRKKQGVDGPLFIGADTHALSVP
                     ASRSALEVFAANGVHAMISTNDETTPTPAVSLAILAHNKGRRQHLADGIVITPSHNPP
                     DNGGYKYNPSNGGPADVKVTGWIQNRANKLLEAGLKGVKRSPYDKALTASTTRRYDFL
                     GAYVTALRSVVDMDVLKSATLHMGVDPLGGAGVHYWAHIADRYKLNLDIVSEQVEPDF
                     RFMTVDWDGKIRMDPSSPYAMKRLIRLKSQFDIAFCCDTDYDRHGVVTRSVGLLPPNH
                     YLAVLVDYLFSHRSRWGKRAAVGKTLVSSQIIDRVAARLGRGLVEVPVGFKWFVDGLF
                     DGSLGFGGEESAGTSFLRRDGTTWTTDKDGMIAALLSAEMTARTGIDPGEHYKRLAHD
                     LGEPVDTRFEAPATPEQKQRLAAISARDVHITDLAGEKVDAILTRAPGNNAPIGGVKV
                     VSRNGWFAARPSGTENIYKIYAESFLGENHVQRLVAQAHDVVDKALGAGARSQTASRQ
                     GGRRAGKDKA"
     gene            38709..40055
                     /locus_tag="VFR20_10705"
     CDS             38709..40055
                     /locus_tag="VFR20_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEU0231033.1"
                     /translation="MSRGASRSGDEIARRKSSGEAELELLQHRSFSYFLHESNQSNGL
                     VLDKSAPGWPASIAATGLAMTCYPVAVERRLMSKAGAVERTLAALRFFWNSHQGPEPD
                     ATGHNGLYYHFLDLKTGRRAWQCELSTVDTAFLLAGALAAAQYFQENTRAQAEIRELA
                     DALYRRADWAWAQNGGETLTHGWRPETGFIPYRWEGYDEALLLYTLALGSPTHPLTDA
                     SYGTWAETYRWDSSYGYDYVYAGPLFTHQLSHCWIDFRGIQDAFMRGKGIDYFENSRR
                     ATHVQYEYAMANPHKFTRYGEFCWGVTASDGPGPCTRKINGVTREFFDYVGRGVPYGP
                     DDGTVAPWVVVASLPFAPDIVLPAIDHYVNELKLSARNLYGFKATFNPTFATPETEPY
                     GWVSPWHFGINQGPIVLMIENYRTGMLWDLMRTCPPIVNGLRRAGFTGGWLEESAA"
     gene            40583..40879
                     /locus_tag="VFR20_10710"
     CDS             40583..40879
                     /locus_tag="VFR20_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU0231034.1"
                     /translation="MKHLHAGHSIETVSVRVSGKVQGVGFRAATVRQAHLLGIAGWVR
                     NASDSSVEAVLQGSVDQIDRMLSWLSVGPPAARVDQVEHQQEFTIRHFDHFEQL"
     gene            complement(40980..43448)
                     /locus_tag="VFR20_10715"
     CDS             complement(40980..43448)
                     /locus_tag="VFR20_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231035.1"
                     /translation="MPHPSKVDGPSLSTLLLRVLGSLAVIVFPLAIVAALPALWGAAV
                     IAALFALVLAAFRVAGRKPARAVLDWIAGAALGLGVRVWLLFPFWWGPIPGIAVVALV
                     FFLQVSLAQRLSLELPGAGPPGGTRADRHFQRRQTPEGQPLRFLDWGEVAMGYGAFHS
                     ILFPDGVVLQGVGDIGRFSVDGRYFAAICPSRGADGLIILDRETRRLYQGTNRDGFWA
                     LEDFAESPANDKPREGAGPSPVFGDFLARTQASDLMRVGDLWLDPQQWSHRLENLVTH
                     EYLSPDHQHKLVARHFVPASLRDLEEPLKPLYEPQYALWLDGEDTGLLTDAPESVSWR
                     ADSQALACRARQATEPAQSLYELRNFWLWENGKNWRALPSVWASSSGEPALTEDSAAT
                     LDARELRLRATLANPELSHGNHGYSIACLRDKSAILIGHDDDGRMRLAGYPCRPVRLC
                     LPLDSPGGRGAARVKSCEFQNGEQAEFTWIRDNAAGLGAWHCRIGDWHLPGLWLLDHR
                     VSDDGRRIALCPFPDIAPVAETFRIATPTTRELQDGPDMLISCLVDFYQDDVSVVEIA
                     GRVDRKPEPGEPDGTPLDPFQTPAPVPALADDFFRPCTNRKDDAVRYYYRLHRFRVGD
                     VIVALPRERTVTQAQAAYGDGDFIYPCPNGADAAWFFGGGVSYGVGWPPAGEPRSGGY
                     LLTASGCAVGDLAPPMSWSEDGRLLALCRLLHLPDGKRQWRLLLLDPAYGMLRVGTRS
                     LGGMPIFERFDAEGLSVKVLNADWLEEGASATTRRFSLRQLTGMPAVALNDEAGLRLA
                     PDQEQNRALWSKLDKLPLAPYRSA"
     gene            43736..44899
                     /locus_tag="VFR20_10720"
     CDS             43736..44899
                     /locus_tag="VFR20_10720"
                     /inference="COORDINATES: protein motif:HMM:NF025021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEU0231036.1"
                     /translation="MKSLFAALIILVALIALAIGVPLILQPDTGGFLSGIGVVLGLVF
                     LAAGNLLVLALNLAFRHFYGAPRWLRVLIRIQLVPAAVSLVMLAVQLYDSWQEGRAAD
                     QHVALYLAIKSNDVGRFDQAQRQCGKQCSSDYSLNAQLLDAADTRAGNVAEALIRQHA
                     KVSTELGQATVSLRTCEGDYLADLNALGVAVARHDASMVDELFPVSDKGARRKALWVA
                     ARLNHLDLVKDLAAKGVPLTIRGPILNENDTLLVAAASGAALEVGKWLLDTQYMPVNA
                     ILGGPDPYPGTSPLQALMRFQSSAPDSPRIKPFLQMLVAHGAKLDVRDREGRTPLQEA
                     IRMKDKRLAQMLLSVGAGKSSLTAEEGKALEALLAQPGPLVSPPHSGGDCVAP"
     gene            45013..45327
                     /locus_tag="VFR20_10725"
     CDS             45013..45327
                     /locus_tag="VFR20_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231037.1"
                     /translation="MRTFVLVIASLLLSLLASIVAALGVALVFLLVWEAFDLCAAFGG
                     PMEGSCGYAFSFFFMPIIVLSCLIFVTILAYRKIHPWLVRRLGPVNPARCAALDHHNP
                     PV"
     gene            complement(45278..45982)
                     /locus_tag="VFR20_10730"
     CDS             complement(45278..45982)
                     /locus_tag="VFR20_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231038.1"
                     /translation="MPSDTSATNPTQLRAKKPSSSFIAFSLLMAVFAFAAAITAFNPH
                     IGVYLRTPHWSMDHLTLLLATLALASGISAVVGRRASRTLLAWSTSCAAWLLVVVVAL
                     LFLFRYPAEFYVQHNKNVVEDHVPEVLEEQALVFNHSSKRPLWGSRTTYGLTWLGPAS
                     ACVAPALAQPGQCKLMECVVDRSHWLAGARIDHSWNVQPFSDADELRDVQCHEVDPAK
                     IKPGDYDDQGRRSVPG"
     gene            46168..49080
                     /locus_tag="VFR20_10735"
     CDS             46168..49080
                     /locus_tag="VFR20_10735"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HEU0231039.1"
                     /translation="MPRPVSRNHLAPLLMLAMLAMPVSPAMAQRGSCVSEDPYAAIKA
                     AWPDPTRSRLGNVCINALHDGRASVRLPSAGVNIDDAAASDGSTDKWGYLDKDGKLVI
                     PPTFDMALNFRHGLAAVSMNGLWGYVDLNGKWAIKPRFLEADAFTSLGLAMVREGVSY
                     ELIDRSGTMVGKPFPAGVKAYLAGGKPGFVYLSYGPEWRSSAGATFSPPDGVFVVKPL
                     GGQDLFIARSASRRYGVMDANGKWIVLPDFQSLDASVIPDGLALARGYRRSALIRDDG
                     TYATDRHVKLIRRLGRHFWLTRDNHGVSRLLDEHGKVAHDFGKAPLGSSLVTLGPFVL
                     WSTDTDWQVFVPGRAAPVSIPRALQPKWSGPENGVLLWRNLDTDAVAGILTRAGRWLV
                     GPGWIADIAFTISLGGQTAFTDRRGSLLNVLDADGNPLLTSDAARGLAHRQVQPLHDA
                     PNSSKTPVAIAILGPCDCDPETGSGLLLSDGHVVQDKAWKSIHSLDPDDSKTSTRVSV
                     AHTTIPQRFAVDTAAGTGMMDVSGKMLLAADQKHIGAFQDGLALVSGRNGTRVMDRAG
                     KLYSVPDYFALESAGAGLLRFRETAKPDALWGLYSVAQAEIAVKPAFKSIGAFESGTA
                     VAQDSSGATGVIDTRGRWVVPARYAAVEPLNRQLWVVRGGAAQTDGMKGVVARDDQVV
                     VTASRGLKVHQNENGLIRVSFQDAEDPSRRGAWLLDQSGKRVMGGSHADIEFMKPWWL
                     VLRPDSGGYVGKDGNWVIPPAPGLGTAFHGSQGAAALAVRNQGKTSLIIDEKGRVHAT
                     LQGGDWAWPDGSDWLVRQVGRDGKEPPETLYADTGGKIRLTVKGAASEFVGDYAVLRS
                     ADGRHYGWIDKQGHEGPAHDYDWLGLPSDGLAFASEHGLYGYLNERGQYVIAPAFKAA
                     TPFVDGRAVVSTRLASMIIDTKGTPLARVTYLCGVRVLFDANNHVEWPKTLPSGCR"
     gene            complement(49085..50605)
                     /locus_tag="VFR20_10740"
     CDS             complement(49085..50605)
                     /locus_tag="VFR20_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEU0231040.1"
                     /translation="MNQAPTKPQTFSHAEVRSIIIGLMLAILLGALDQTIVSVALPMM
                     SADLGGVDLLAWVVSGYLITVAVATPIYGKLGDLYGRRVMLTSAITIFLLSSIGSAMA
                     PSMPFLVGARILQGLGGGGLISVAQAIIADVVAPRERGRYQGYISAAFAVASVSGPLL
                     GGFLTSYLSWRWVFWINLPLGAAAMFISRRALARLHTPRIKRPVDYPGAILMTVALSP
                     LLIGITRVGQGSPWMDELNVWLFGGTLVAIALFLWQETRATEPIVPLGLFRNPTVSLS
                     CTLMFIAFILIVSLSVLIPLRAQMLLGAQADQAALLLLPFSLGIPVGAYVGGRMSAFT
                     GRFKRIQLCGAVCTPIAMLGLVFVDAHATGANLVCVGVIGFTIGLQLPTSTVAVQNAV
                     ESRHMGIATAVIAFCRSLGAAIGIAVLMAVLMATLQAMAPATAGGLSGPEIIKSLIDQ
                     AASAMDALVRADLVSSVDDAFRKVFALATATACVSILLSLLMRDEELGDTAPAAAD"
     gene            50832..51254
                     /gene="arfB"
                     /locus_tag="VFR20_10745"
     CDS             50832..51254
                     /gene="arfB"
                     /locus_tag="VFR20_10745"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEU0231041.1"
                     /translation="MHHVNDQIVLDDRDVTFTMIRAQGAGGQNVNKVATAVHLRFDIS
                     ASSLPDDCKAVLLALRDRRVSGDGVIVIKAQSFRTQEKNRADAIERLLALIRGALKQP
                     APRRPTRPTRASQRRRARNKARQGEVKRLRAKVAEAPE"
     gene            complement(51315..53147)
                     /locus_tag="VFR20_10750"
     CDS             complement(51315..53147)
                     /locus_tag="VFR20_10750"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEU0231042.1"
                     /translation="MLTALVYLAAGVVAVPIAQRLGLGSVLGYLVAGVIIGPLLDVAG
                     NTSSVMHVAEFGVVIMLFLIGMEVRPSMLWRLRGVFFGLGGAQMAVTGLVLAAACLVL
                     GQSWQAAVALGLILSLSSTAIVLQTFEEKGLRHTAAGSAAFGILLFQDVSTIPMFAAL
                     PLLAAEHIAPTAASTGLLAGQPQWVHGLAVLAAVLLIISAGRYLTRPAFRFIANTRSR
                     EIFTASALLLVVAVDALMRTVGLSAALGAFLAGVVLADSEFRHELESDIDPFRALLLG
                     LFFISVGAGINLGLLVSQPLTLLLCVVSLMAIKAVVIYGMARLFGMEQRSAAMMGAAL
                     AQGGEFAFVLLGLVAMLGIIPGQIAALATATVAISMALTPLLIMGYQKWCTQGFSPST
                     GARPENEAFDSRPDAIIAGFGRFGQIVGRLLIVNGFKTSTLEASAEQVELVRRFGRRV
                     YYGDATRLELLQAAGAADARLLVVAIDDRDQAEELVETARANFPNLKVLARAFDRRHA
                     YRLLDKGAHIIERETFEASLTLGAEALKVLGFKGTQAVRAAKLFRKHDVNMFEALAEV
                     WGDEERFTIATRESSERMNELLAADIRDMLKEPDSDAERERAQA"
     gene            complement(53230..53739)
                     /locus_tag="VFR20_10755"
     CDS             complement(53230..53739)
                     /locus_tag="VFR20_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU0231043.1"
                     /translation="MDKIPCELAPAWVVSEWLNTPTPITLESLRGSVVVLHAFQMLCP
                     GCVSHGLPQARAVHAAFPQDKVKVIGLHTVFEHHDAMTPVALRAFAHEYRLRFPIGID
                     QAAQDGDPIPMTMRAYRFEGTPSLVVIDRNGGIRLRHFGQMDDLRLGAVIGQLLTEPD
                     IVENPELTR"
     gene            complement(53919..55076)
                     /locus_tag="VFR20_10760"
     CDS             complement(53919..55076)
                     /locus_tag="VFR20_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010118444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU0231044.1"
                     /translation="MSRPTLLRRIPLLLLLAHLYVLVRLIEPALGTRVQWVVPVVIAA
                     LYALILAGFLARRQAGRLSGDVLSWSGFLALGCFSWLFVLTLLRDAVLFLLMVGSAIT
                     PSLKEATNHAPAITALAVPILTMLAVLLGLFNARRMPRLVDVDVPIPDLPDSLHDFTI
                     AQISDLHVGSTIKRPYVRRVVDAVNRLKPDIITLTGDLVDGSVALLASDVAPLSGLQA
                     KEGVYAVTGNHEYYSGAGAWVAEYRRLGMDVLMNQHRVIHRGGALLVVAGVTDFTAER
                     FDSRQACNPVAALRAAPREAAARILLAHQPRTAADAAKAGFDLQLSGHTHGGQFWPWP
                     YFVSLQQPYVAGLHRLDKLLIYVSRGTGYWGPPMRLGARSEITRLRLRRQA"
     gene            complement(55099..56784)
                     /locus_tag="VFR20_10765"
     CDS             complement(55099..56784)
                     /locus_tag="VFR20_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU0231045.1"
                     /translation="MNQSRTGLTWSLIVIVGLLTLCPVLMLLLGSFSQGLTAFGHFTV
                     AKYVSAYTDPYLLKVIGNTIIFVLGSTLLATLLALVLAYLNTRTNIPCKPMFTVLSIV
                     PMMIPHLLFAVSWALLLNPSNGLLNLFLKDALGRHGAPFNIYSLEGMILVEGLLNMPI
                     AYLIIAPAMASFDVALEESSRVSGAGVWRTLFRVTLPVLRPAILSAFVLGIVRSLASY
                     AVPRVLGMPGRVDVLATYLYEMIATGFAPDYGKAAALGMSVLAASIGLIVLYRALTAE
                     GGKFVTISGRGYRPSVIDLKRARTPLFIAIGVLSFLMAVLPVAVLFYTSLVPYSMVPS
                     AHAFSMMSWKHWAEVLQDPASMLALRHSVFLAIVGATLGVLLSIFIAYVIVKIRTRAA
                     GLLESLSFLSFSFPGIVIGIGFMWFFVQTPLYASLTALLVAYIATYLPYGVRPLASAF
                     VQVHAHLEESSQVCGASALTTMRRIIVPLLVPGIVSAWILMATMFVRELTVSVVLSRP
                     GSEVLAVQILNFADDGLWGKLSALGIVMILISTGLVLLATYAGTRFRAARGDS"
     gene            complement(56797..57939)
                     /locus_tag="VFR20_10770"
     CDS             complement(56797..57939)
                     /locus_tag="VFR20_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0231046.1"
                     /translation="MEIRIEGLVKNYISEGKSVRALDGIDLTIPANQIFTLLGPSGCG
                     KTTLLRCIVGLETPDDGEISIGGEVMWSRSKNITVPPEKRGLGMVFQTYAIWPHMTVF
                     DNVAYPLQVRGERRATIREKVAKALSFVQLEGVENRSATRLSGGQQQRVALARALVAE
                     PKVILFDEPLSNLDAKLREETRKELRHFLAGLNITAVYVTHDRVEALALSDSIAVMRG
                     GHIIERGSPQKIYFDADHRFVADFIGRANLVSATVREQRADATVVDSPLGVMRCQKRD
                     LPVGAQATLCIRPEFLLLTNADETHGANQGSVTASTGGGDGTDVVHNLISGCIETLDF
                     VGEVYEAEVRVGDQLLLARVDPGVRVAQGDMVRFALTPAHCLLVSA"
     gene            complement(58015..59046)
                     /locus_tag="VFR20_10775"
     CDS             complement(58015..59046)
                     /locus_tag="VFR20_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0231047.1"
                     /translation="MNAFDRREFLKLAGAAGVAGLAGALPSVSLAEAVSAKAKAEGKA
                     VFYANITSVESVMKAFDAATGIKGQYTRISSSKFLPTVLTEFNAGKLLADVVQAPRPM
                     LELLKEKGVLAAYQSPSAKGYPDWAVKDPRMTLFGIEYVSYLYNTDHVKRADAPKRYE
                     DLADAKWKGQIVMANPATHSSTISWLIGLKEFVFKSESDWMAYVKGLAANQPMFVASF
                     GPTPAPIESGEKLIGISMPKYIVTKAPAPLDWGPRGGQPLLGTPRAIAVTSKAPRPDA
                     ARAFMDYWLSKEAMSLLAKNVGEYVLAPGVFPAIDGIDKVKVLPVRDLSDTEIEKWGR
                     EFGKIFGSR"
BASE COUNT        11165 a        17739 c        19024 g        11567 t
ORIGIN      
        1 cgccccccac cactttgcta ccacacagag agctggttac ttgaccgctt tctggaaacg
       61 caccagcagt tcgtcgccac gtttgaccat gtcgtcgaga ccggccttgg ggctgatctt
      121 gccggcgaag atattctcca tgtcgccgtt ttcgatgtcg cggatctggg gcagataacc
      181 cagacggata ccgcgcgagt tcttggtggt gttggcgtcg agctgcttga ccgcgacttc
      241 ggtgccgggg ttcttttcat agaagcccga ctttttggtc agttcgtagc ccgccttggt
      301 gactggcaca tacccggtat cctggtgcca ggcggcggcc tgtttggggc tggagatgaa
      361 cttgaagaac gctgtcacgc ctttgtagac cgcgggtgat ttcttggcga acacccacag
      421 cgacgcgccg ccgatgatgg agttctgcgg cgcgcccttg acgccggtga ggtagggcag
      481 ttccgacgtg ccgaacgaga agttgccgtt cttcacaatg ttggcgcggg taccgctgga
      541 acccgtgtac atgccgcatt tgcccgagat gaacagtgcg ttggcggcgt cgccccggcc
      601 gccgtacgtg aatgtgcctt ccttggacat cttctgcagg aaggtcatga agcggacgta
      661 gatgggcttg ttgatttcca ggcgtgcgcc cagaccgccg aagccgttgt tttctgaggc
      721 gtacggaacg ttgtgccagg cgctgaagtt ctcaagcagg acccaggcgg gccaagcgga
      781 ggtgtagccg cactccatgc cggcggcgcg cagtttcttg gcatcggctt cgacgccttc
      841 ccaggtgcgc ggcggcttgt tggggtcgag gccggccttc ttgaaagcgt ccttgttgta
      901 atagaaaacc ggtgtcgaac tgttgaacgg catggagacc aatttgccgt tgggggacga
      961 ataatagctg gccacggcac ccaggaagtc cttggggttg atggggttgc cggccttgtc
     1021 ggacatttcc tgaacgggtt cgatggcgcc cttggcgtac atcatggtgg ctgtgcccac
     1081 ttcgaagacc tgcaggatgt caggggcgtt gccggcgcgg aacgcggcga tcccggcatt
     1141 catggcttcg ttgtagttgc ccttgtagat ggccttgacg acgtattcgt tctgactctt
     1201 gttgaattcg tcgaccatct ggtcgacgcg ttgcccgagc ggaccttgca tcgcatgcca
     1261 gaacgtgatg gtggtcgccg ccgaggccgt ccccatcgcg gcgatcatgc cggccaagga
     1321 cagggatagt gcaaaatgct ttgctcgcat gagccattct cctagaaatg ccggccgttg
     1381 ttatggaagg atggccggct tcgttcagta gtctatgaca cgctgtttac aatctgatga
     1441 attcccggat gatcagaaaa gtgtccagat aacccggaat gtacagataa tccgcccgcg
     1501 tggtcaacga gagattacgt gaatcgtctt gggtcggggc gggcgttgac gatggatcag
     1561 gctgaggggt ggcgctgaaa aaccttacaa tggcggcccg ggtcgatatt ttttttaaaa
     1621 acttggtgtc cgtatgacga aagaactcaa gctcgccttc attggcggcg gcaatatggc
     1681 cagcgcgctg gcgtcggggc tgatcggcaa gcgttgtggc gcgtccgatg tccatgtcat
     1741 cgatccgtac gcacccaccc tggagggctg ggcgcggcgc ggggtgtcca cggcacccgc
     1801 cgccggcccc gagctcgggc ggcacgatgt ctggatcttc gcggtcaagc cgcaggccat
     1861 gagggaagca gtcaatgcct gccggccctt ccttcgtccc ggcacactgg ttgtcagcat
     1921 cgccgcgggg gtgcgcgccg acaccatggc cgaatggctg ggtggcgacc atccccatac
     1981 ccggttggtg cggtgcatgc cgaatacacc ggccttgatc ggtgagggcg tgagtggtct
     2041 gatggcgttg tcgggggttt cgacggaaga caagaccctc gccgagcagc ttctccgcgc
     2101 ggtgggtgaa gtggtatggg tcgatagcga cgccatgata gatgcggtca ccgcgctgtc
     2161 gggcagcggg ccggcatacg tgttcctgct cctggaggcc atgatcgcgg cagcggtatc
     2221 gcagggcctg gcggcggacc aggctcgtac gctggctctg ggcaccctgc gcggcgcgac
     2281 gcagttggcg gcgctgtcac cggaaacgcc cgcgcagttg cgcgaacggg tgacgtccaa
     2341 gggcggcacc acggcggcgg ccctccaggt cttccgtcag gaaggcttcg aatccatcgt
     2401 cgcgcgggcc atggcggccg cgcgtgatcg cgcggccgcc atggccgccg aatttcactg
     2461 aagtagaacg gcggcttcag tttgcggtgc gatgcggtga ccagatgaaa acaaggtttt
     2521 gacgactcgc gctgccaacg gttgcggcgc ggtgtcgttt caggggttct ccgtagtgac
     2581 agtcgggcca catgtgccca gaatacgcct gccgtcgtat aaatcggcat ccagaacaag
     2641 ggctcgcggc agcaacggtt tcccccacga agggtaaaag ccggttgtac gggggcacag
     2701 aggggaagtg atctcatgga attcataaca ctggcattgc cggcctcgcg ccggccgcaa
     2761 tccggcatag cgtgttttcg cgccgcccac gatttccgat gcgggtattc gtcccgcttc
     2821 gcgcccacac agtcaactgg cgcaatacct gttctcatcg ccttcggcgt ggcgcgcaaa
     2881 cccctctcca cgtgagcaaa ccctagccag gcccattggg catgacggct tcatcccggg
     2941 aaaggggtgg ggctttcgat tgtccgtttg cgaccatgtg gcggtgttcg ccgccgatat
     3001 tcaggaagat tcgtttccat gtctgacttg cttccccaac ttacgcaaca attcttcaac
     3061 ggcctgtcgc tgggagccat ctatgcgttg atagccattg gatatacgat ggtgtatggc
     3121 atcatcggca tgatcaactt cgcccatggc gacatctaca tgattggcgc gtacgtaggg
     3181 ctggtgacgt tgtcggccat cggggccaac agcggcctgc cgttgccggt ggtcgtgctg
     3241 atcatgatcc tggtcgccgt ggctgtcacc ggggtgtacg gttacgcgac ggaaaaactg
     3301 gcgtatctgc cagtgcgtca cagtccgcgg ctggtgccgc tgatttcggc catcggcatg
     3361 tccattttcc ttgaaaactg ggtggccata ggccaaggcg cgcgtgatct ggctgtgccg
     3421 caactcatca aggggtcgtt tcagttcacc ctgggagcct cgacattcca ggtgacggca
     3481 tcgtattcgc gcatcatgat catcgtggtc acggtgctgt tgatgatcgg attgtcactt
     3541 ttcatcaagc attcccgcac gggtcgggcg tcgcgcgcct gctcacagga tctgcacatg
     3601 gccaatctgc tgggcattga caccaaccgc atcatttcct tcacgttcgt gctgggcgcc
     3661 atgctggcgg cggtgggcgg tgtgctgatc gcgctggcga tcggcaagct caatccgttc
     3721 attggcttca tcgcgggaat caaagccttc accgcggccg tgctgggagg catcggcagc
     3781 atcccgggcg ccatgctggg cggtgtgttg ctggggctgg ctgaaacctt cgccgccgcc
     3841 tacatctcat ctcaatataa ggacatcgtc gcctttggcc tgctggtgtt cattctgctg
     3901 ttccggccca ccggcctgct gggcaaaccc gaagtggaaa aagtctgatg gttaaaagca
     3961 atttcaccaa cgcgctgatc gcggcgctga tgtccggcat cctgatcgcg ccggtgttcg
     4021 gctttcgcat catcacccat ggcatgcagt cggatctgca accccagtgg cccatgatcg
     4081 tcatgggcat ggtgatcgtg ttcgtggccc agctcatgcg gccggcgatc aagtcgtcga
     4141 tgagccgccg catgagcctg ccgtcgctgc ccgcgatcac cgattccgtg cggcgcaaag
     4201 gcctggttct gctgattctg atcgcgctgg tgtggccgtt ctttggcaat cgggcccagg
     4261 tcgatctggc caccctggtg ctcatctaca tcatgctggg cctggggttg aacatcgtgg
     4321 tgggcttcgc ggggttgctc gacctgggtt ttgtggggtt ttacgccgtc ggcgcctata
     4381 cctatgcctt gctgtatcac tgggccggct ggagcttctg ggaagcgctg cctctgtccg
     4441 gcgccgtggc ggcgctgttc ggattcgtgc tgggtttccc ggtgcttcga ctgcgcggcg
     4501 attatctggc gatcgtcaca ctgggattcg gcgagatcat ccgtctcatg ctcatcaacc
     4561 tgtatgggct gaccggtggc cccgacggga tctcgggtat tcccaagccc accgtgttcg
     4621 gttatcccat gctgcgccgc gccccggaag gcgtggtcac atttcatcag ctcatggggt
     4681 ggagcttcaa caatcaggac atcatcatct atatgtacct ggcgacattg ttgctggtct
     4741 ttttcgcgtt gttcgtctcc acccgggtca tccgcatgcc cataggccgg gcctgggagg
     4801 ccttgcgcga agacgagatc gcctgtcgtt cgctggggct caaccccacg cgcatcaaat
     4861 tgtcggcgtt caccatggga gccacgttcg cggggctggg cggggcgttt ttcgccgcgc
     4921 ggcaggggct ggtcaatccg gaatccttca cgttcatcga gtcggccctg gttttgtcca
     4981 tcgtggtgct gggcggcatg ggatcccagc tcggcgtcat cctggccgcc attctgatca
     5041 ctgtggtgcc cgagttggcg cgtgaattcg cgcagtaccg catgctggtg ttcgggctgg
     5101 tgatgatctt gatgatgatg tggcggccgc agggtctgct gcctgtgaag cggccgcagg
     5161 tggagctggc atcatgaacg acacgttgct gagtgtttcc ggtctgacca tgcgcttcgg
     5221 cggactgctg gccgtcgacg cggtcgaatt cgacgttcgc cgtgacgagg tgttcgccat
     5281 catcgggccg aacggcgcgg gcaagaccac ggtgttcaac tgcgtgggcg ggttctacaa
     5341 acccaccacc gggaccatca caatggatgg caccccgatc aacggtctat ccagccacaa
     5401 ggtggcgcgc aaggggctgg tgcgcacgtt tcagaacgtg cgcctcttca agagcctgac
     5461 cgtcctcgag aatctgctcg tggctcagca catgcatatc cagaccggtt tgctgcaagg
     5521 attgctgaaa gtgtcttcgt atcggaaatc cgagcgcgac gcgctggatc gggcggttga
     5581 atggctggaa ttcatgggga tccgggcgct cgccaaccgt gacgcgggaa cgctggccta
     5641 tggccaccag cgcagacttg aaatcgcgcg ctgcatgatc acccggccac ggctgctcat
     5701 gctcgatgag ccggccgcgg gcctcaatcc ccaggaaaag cgggatcttc aaacgctgat
     5761 cgatcggttg cgcagggagt tcggggtcgc cgtgctgctc atcgagcacg acatgagcct
     5821 ggtgatgggc gtgtccgaca ggatcatggt gatggagcat ggccgcccca tcatcacggg
     5881 tgggcccgcc gaggtccggg cggaccctcg ggtcatcaag gcctacctgg gggaagaata
     5941 aatgctgaag ctggaaaacg ttcacacgca ttatggggcc attcaggcgc tgaacgatgt
     6001 gtccatcgag gtcaaccaag gtgagatcgt cacactgatc ggcgccaatg gcgccggcaa
     6061 gacgaccctg ctgatgacgg tctgcggcac gccccaggcc agtgccggac gcgtgttttt
     6121 cgaagaacgg gacatcaccg ccgacagcac ccatatgatc atgcgccggg gcatcgccat
     6181 ctcacccgag gggcgtcgcg tttttcccga cctgacggtg tcggaaaacc tgaaaatggg
     6241 tgggttcttc ctgagcaaga tggaaatcca cgcgggcctg gagcacgtct atggcttgtt
     6301 tccgcgtctg cgtgaacgcg ccaatcagcg cgcggggacc atgtccggtg gcgaacagca
     6361 gatgctggcc atagggcgcg cgctcatgac gcggccaaag ctgctgttgc tggacgagcc
     6421 cacgttgggc ctggcgccgc tgatcatcgc acagatattc gacatcatcc gcaccatccg
     6481 cgagcaaggt gtgaccgtgt tcctggtcga gcagaacgcc aataaagcct tgcaagtggc
     6541 cgatcggggt tatgtgctgg aaacgggccg ggtggtgctg gccgactccg gcgccaatct
     6601 tctggtcaac gatgacgtgc gcaaggcgta cctgggcggg tagcgccacc cacccagacg
     6661 cggcgcatca ggacaggcct tcggccttca gcgcggcttg cacaccggtg tcagccgaca
     6721 tgtgttggaa gaaggcttcc aggtcggcca tgccggccag gtcgacctgt ttgcccttgg
     6781 cccagcgcat caccgtgtac aggtaagggt cagcgatcga gcgcttgccc gtgagccagg
     6841 tcttaccttt caattggtca ttcaggatac cgaacaacgt gtgcagccgg ttcatgacgt
     6901 tgcggaccaa ctcttcctga accttggggt cggacacgta cgccgccgcg ctgaacacca
     6961 ggctgaacgt ccggtgcagg tcggaattgc agaaccccag ccagcgccgg gtttccgcgc
     7021 gctcttcctt ggtcttgccg accaggtcgg ccccggggtg gagttcggat agatattcga
     7081 gaatggccgc gttctgggtg agcgtgatgc cgtcgttttg cagaaccggg acggcgccca
     7141 ggggattcat cttcagatag gcgggttctt tgatttctgt gcggctgacc tgctggagtt
     7201 cgtagggcgc gcccgtccat tcaaggacga tgtgcgcggc cagggggcag gcgccgggaa
     7261 gataataaag tttcatggtt gaagtcgatg gctgatgatg tggctagggt gtgcggtttg
     7321 agtgattcgt gcattcaaga cgaaaacact ggcggagcgg cacactcgag ttccgggatg
     7381 aacggccttc attctatcgc catttcccct gggatctttt gagcgggtca tcgcgccgca
     7441 ggcatgcgtg gatggggggc cgtcatccgc gggggcccag atgtttgtcc agaaacgctt
     7501 ccatgcgttc gtagaattcg aacttgttct cgtcgttgtg gaaaccgtgg ccttcgttgt
     7561 ccttgacgag ataatccacg tccacaccgc gttttttcag cgcggcgacc atctggtcac
     7621 tctcggcctt gttgacacgc gggtcgtgcg cgccctgtgc gatgaacagc ggggtgcgga
     7681 tccggtcggc gttcagcgca ggcgacgtgg ccaccaggcg gtcgcggtca tgcacggggt
     7741 caccgaccat gcagtgcatc ttttccagca tgggtttcca gtaaggcgga atggtgttca
     7801 tgaaggtgag caggtttgac acccccacat agtcgattgc cgccgcgtac aagtcgggcg
     7861 tgccgacgat gcctgacaag gtggcataac cgccgtagct gccgccgtag atggcgatgc
     7921 gcgccgggtc ggcgatgcct tgatcgatga gccaccgcac cccgtctgtg acatcgtcct
     7981 gcatggccag gccccattgc ccgaagctgg cttcccagaa ttttcggcca tagccggtgg
     8041 aaccgcggaa attcatctgc agcaccgcgt agccgcgatt ggccaggaat tgcgcctcgg
     8101 ggttgaaccc ccaatgatcg cgggcccagg ggccgccatg cgggttgatc acgcagggca
     8161 gggcgcgcgc gggccgccct ggcggcaacg tgagatagcc gtggatcgtc agaccatcgc
     8221 gggcagtgta ggtaacgggg cgaacgggcg ccatgtcggc ctcttgcaac gcggggttga
     8281 tatcggccag tttgtcgagt gtgcggtttt cggcgtcgta cagatagcgt gcgccaggtg
     8341 tacggtcgtt atacgcggcg acgatgaatt ttgcctcgtc gcgtgacgcg ctctgcaagg
     8401 cgacttcgta gcccggcaac cgtgcgcgga ggtcctcatg gcgccgtgcc gccacgtcgt
     8461 cgaagaaatg gtggtgcggg cggtcggtgt tgaacgaggc ttcggtcagg actttccgca
     8521 ggcgcgagta gcttgcgcca tcgagatcga cttccggggt ttgatacacg agccgttcgt
     8581 cgtccggccg ttccgggtcg atgacgacca gggcaaggca atccctgcct cgattgctca
     8641 acgcatacag tgagcgattg tcgaaggtga agaacagcgg acctaccgag gtgcggaagt
     8701 cggtgtgaat gatcgggcgg aaaggttgcg actcgtcgtt gcgatacagc acggtggtgt
     8761 tgagcccgtc actggcgatg gcggcgcgaa tgcggccctc gtggtcggcc tgccagccaa
     8821 cgatgttgcc ggggttttcc gccaccaggg ccagttcacc ggtgcgcagg ttggcgcgat
     8881 agacgtcgaa gacctccgcc cgacgcttgt tgtggctgat cagaatgtgg tcgggatcgt
     8941 cctcgagatc gtcctggacc ccagcccgca cgcctggaaa aggcgtcaga tcggtgatgg
     9001 cgccgctggc agtgtcgacc gccacgacgt ggaagttctc gtccccgttg aaatcctttt
     9061 cgtacaggat ggtgtcgttg cctttccaga agaaaccact cacatcgcgc tcggtttcgg
     9121 ccgtcagcct tcgagaggct ccggttggtg tgctgccggc cagcgtctgg atgaagacgt
     9181 tcatgcgcgg cggcttgccg tctatggaca cgggctccat gaaccccagt gtggcgccat
     9241 catcggacag gcggaatgag ccacgttgcg gattcttgaa aaagtcccgg atggggtagc
     9301 gggcagggag ggctcccgca ccctcggagg cttcgatcat ggaagacgtg gcaaaccgga
     9361 acaggttggc gagcggggga aaaagcatga cgaactcctt gcgacagcct tgttgttgtg
     9421 ttctgggtct tcgggtgtga agcggaagtc gcggcacact gcccgggtgg ttcggaagac
     9481 tggatcgtgg tgtgaaaccg ttactatatc ggactgatgg cgtcgccgag atggtgacgc
     9541 catgaagaca ttccttggaa ccattccatg tccgaaaagt tcacgcgtat cgtgtttctc
     9601 gaccgggaaa cgatttcccc gcagaccgtg ctgcggccgg cgtcgtttcc ccatgatctt
     9661 gtcgtgcatc agcatacggc cccgggtgac gtggcggcgc gcatcgccga tgcggacatc
     9721 gtgatcacca acaaggtcag gttgggccgc gaggcgctgt cgggcgcgtc gaggctgcgg
     9781 atgatcgccg tggccgccac gggaaccgat gtgatcgacc tgccagcctg cgcggatcat
     9841 ggcattgtgg tgtcgaacat ccgtggttat gccgtgaaca ccgtgccgga gcacactttt
     9901 gccctgatct tcgcgttgcg ccgcagtatc gtggcatatc gggagtccgt caaggcgggg
     9961 cgctggcagg aatcaggcca gttctgctat tttgattacc cgatccgcga tctggccggg
    10021 gccacgctgg gcatcatcgg tggcggcgtg ttgggtcagg cggtggccac catcggccgt
    10081 gcgctgggca tgagagtggt cttctcggca cgcaagggtg tggcgcaggc cgatgcacgg
    10141 tacaccccgt tcgaacaggt gctggctcaa agcgacgttc tgaccttgca ctgcccactg
    10201 aacgatgaga cgcgcggcat ggtcggtgac aaggagttcg ccctcatgac gcgcaagccc
    10261 ttgctcgtga acaccgcacg cggcgggctg gtcgatgagg cggcgctcga gcgtgccctg
    10321 cgctcggggc ggctgggtgg cgcgggcttt gatgtcgcca cccccgagcc gccttccgga
    10381 gacagcgtcc tcatgcgcct gctggatctg cccaatttca tcctcacccc ccacgtggcg
    10441 tgggccagcg acgaggcgat tcaatcgctg gccgatcaac tcatggacaa tatcgatgcg
    10501 tttcagtccg ggacgccgcg caatgtggtg gccgcgccgg gagcggcgcc gcgcaagtag
    10561 cgcaaggcca gcgcggccgc caccagatag cccatcgtga tcaagccgaa ggtcacgggc
    10621 agggagcgcg gccatgccgg cgcgctgtgc aagatcgttc caatgagcgc cacgccgatc
    10681 agggcgccga tctgacgatt ggcgttcaat gccgccgccg cgatgttggc gtgatcctgg
    10741 ccggccactt ccatgacagt cactgtcatg gcgggtatgg caatgcccac gcccaggttg
    10801 attagcatga tcagcgcggc aaagagccaa tagggcagta tggtcgccgt cgcggtcacc
    10861 accgcggtca atgtggcggc ggacgccagg cccaggctca gcggcatacg agtgccccag
    10921 cgcgcgccga cctttccgga cgccaggttg cccagtgtga acatcatcaa caagggcagg
    10981 agatccaacc cgatgcgcca ggcgtcgtct ccacgaacct gttgcagata gaggctgatc
    11041 aggaacagtt gtccgaaagc ggccagattg atgaaaaaac ctatgccgtt ggccgcggtg
    11101 aaacgcgtgt tgcggcgcag cgtccgcggc acgatgggcg tggcgctgtg tcgttcgcgc
    11161 gcgatgaagg cggtgaaggc ggcagcgcta aggatcaagg cgcagccgat gggcatcgac
    11221 gcccagccgt atgtgccgtt ctctatcatg gcgaaggcca gtgccgcgag gcccgcgacg
    11281 gcgaaaaggt ggcctgaggg attgagcgcc tgacgttggc gcggcgatac cgcgatgtgt
    11341 cggcgcgtga gcagcacgca tcgccgtgcg cactgtttcg ggtggttttt cgaagtcgcc
    11401 ccaaactcga ttcagagtta cgtaacaggt ttagaggatc ataggattat cctagtacaa
    11461 tcgaaaatcc ttctccaaag gaattttcat ggaacactgg cgcgaggcgc tggctgcatt
    11521 tcgcgggggc gaggcaaagt acaagatact cgtccaggcc atcacccggg acatcgaaaa
    11581 tggcagcttg ccagacgggg cgcggcttcc accccagcgg gatgtcggcc gtgagctggg
    11641 catcagcgtg cagacggtgt ccaacgccta caaagaactg gaacgcgatg gcctcattcg
    11701 ttgtgaggtg gggcgcggca gttttgtgtc gcgcaatgtc acggagcagg tgtcgtcgta
    11761 tatgctcgac cacagcgaac gctccatagc cgatttttcc acggctcgca tcattcatac
    11821 ccttgaacac gaacacgcct ggcgtgacat ctgcgcggcc atgtcgcgag aagccgacca
    11881 accctggatg cgcgactgcc gacccatcgc tggattcgat cgtcatcgtg ccgcggcagt
    11941 cgcatggttg gcacacatgg gggtcgagtc cacaccggat accgtgctcg tcaccaatgg
    12001 tgccgcgcac gccgtgttcc tggctctggc gtccctggtg ggatcggatg acactgtggc
    12061 ctgcgagaaa cttaccgatc atggtgtcat tggtgccgcg catgtgctgg gtttcacgct
    12121 gaaggggctc gacatcgatg aatacggcat ccggccagac catttcgagg acgtttgcgc
    12181 caacgaacgc atctcggcac tggtctgcat tcccaacctg cacaatccca cgggcgtggt
    12241 gatgccagag gctcgacgcc gcagcctggt ccggatcgcc cagcgttatg gggtctacat
    12301 cgtggaggac gatgtttatg gcccgctgct cgacacgcgt ccgcccccgc tcagccagct
    12361 tgcgccagat ctcggcttct actgcaccag tttcaccaaa tccgtcatga ccggactgcg
    12421 gattggttat ctcagtgtgc ccacgcgctt tgccctgcgg gtcaatggca tactgcgggt
    12481 caatagctgg atggccacgc cgctgatggc cgaggtggcc gcccgctggc aagaggacgg
    12541 cacggctgaa catctcatcc agatacagag ggagcgcctg gcgaagcgcc acgccatggt
    12601 ggatgagatc ctgggcgatt atgtggcatg gcaccatcca gcgggtctgt ctgtctgggt
    12661 caacgtgcct gaccactggc gcacggagac attggtcggc gtgctcaggc gccgtggcat
    12721 agccgtgacg tcaccagagc cgttctccgt ccgccagtcg catcgcacca aggcgttccg
    12781 tctgtgtgtg ggcgccgaca tcgacgacga cgaaacgcga cgggcgcttg tcgaggtgcg
    12841 ctccacgttt gaacaatacc cgctggccca cgatttcgtc tgacgcagga tgttttttct
    12901 gccggctagt gtttggtctg tggcttttcc cgtccttgat gtgaaatgat ctagtacatg
    12961 agaataataa tattggtgta ctacactcat tcgcaacaca atccaaggtg tcgtgactat
    13021 gatggcgttg tcactcggtg atgtctacca ggcgcggaac aaattggcgg gaagggtgtg
    13081 tcggactccc ctcgtacact ctccagtact gtcgcaactg gctggaacac ccgtccatct
    13141 gaagatggag tcgcaccagc ttaccggcag cttcaagttg cgtggcgccg ccaatgccat
    13201 cgggaacctc gatgaggctc aacggaaatg cggtgtggtg accgcctcta ctggcaacca
    13261 tggccgcgct gtggcctatg ctgcgcgcca gcgtgacatg cgcgccgtgg tctgcctgtc
    13321 ctccctggtg cctcagaaca agatccaggc cattcgcgat ctcggcgcag aggtctgcat
    13381 cgaagggaac agccaggacg atgcgctggc gcgggctgat cggttcgtga ccgaagatgg
    13441 gttggtgatg gtgcctccgt tcgatcatcc ggacgtgatc gcgggacaag gcactgtcgg
    13501 cctcgagatc ctggagcaac tccctgaaac agggtcggtg ctcgtgccat tgtcaggcgg
    13561 cggcctgttg tcgggcatcg cattggcggt aaaagcggcc aggccggcca taacggtcgt
    13621 gggcatcacc atggaaagcg gctgcgccat gcatgccagc ctgcgggccg ggcatccggt
    13681 gcaggtcgac gagacccctt cgctggccga ctcccttggc ggtggcatcg gccttgacaa
    13741 tcgtcttacg ttttccatgg tgcgtgatct gtgcgatcga acgctgttga cgagcgaagc
    13801 ggccatagcc gctgccatcg tgcatatgta tcgccacgaa cgcgaggtcg tcgaaggcgc
    13861 ggccgccgtg ggtgtggcgg cgctcatgga tgggttggtt ggtgatctgg ccgttcaggg
    13921 ccccatggtt ctgatcgtga gcgggcggaa catcgacatg gcgcgacatc tggatctgct
    13981 ggcccgttct gtttcccctc cttactgaga tttctccgag gttcccatgc ccaaggtcgt
    14041 gattctgagc gaggctgatc tgcgcgcttg tgtgtcgctg gatgccgagg cggtcgattg
    14101 cgtggaacgg gcgttccatc tgctcgccac agcgcctgtc gtgatgccgc ccatcctgcg
    14161 tctggacatt gccgaggcca atggcgaggt cgatgtgaag acggcttatc tgccgggtgt
    14221 cgacagtttc gccatcaagg tcagcccggg ttttttcgac aatccgaagc ttggcctgcc
    14281 cagtctgaac gggatgatga tgctgctttc ggcccgcacg ggtctgttgc agtcgctatt
    14341 gctggacaac ggctacctga ccgccattcg cacagcggcg gcgggcgcgg tggccgctcg
    14401 ctggttggcg cgggcagacg cgagcaaagt ggccatcgtc ggggcggggg agcaggccgg
    14461 attgcaactg cgggccctga cgcttgtgcg gccggtgtct tccgcaaccg tctgggcgcc
    14521 caacccggaa aaggcggcca gcttcgccct gcgcatgacg agcctgttgt cattgccggt
    14581 gcaagcctgc gtcgatgtgg acacggccct ggcggatgcc gacatcgccg tcaccgccac
    14641 ccccagcact cacccgctga tcttcgaaca tcatctccgt ccgggcctgc acatcaccgc
    14701 catggggtcg gatgccggcc acaagaacga gatatcaccg accgctgtca aggcggtgga
    14761 tttgtatgtg tgcgatcggc tgagccaagc acgcgccctg ggcgaactga ggcacgccat
    14821 cgcgcggggc gccatcgacg aagacgcacc catgatcgaa ctgggccaga tcattgccgg
    14881 gcagagcccc gggcgcagga gcgatgatga catcacgctt tgtgatctta ccggcaccgg
    14941 ggcgcaagat acagccattg cgacattggc ccatgcgcgc gcactgcgca gcggccttgg
    15001 actcgatttt caatcctgac aacgtatgga tctgacacca tggatactct tgacattcct
    15061 ctcgcggtgg acgaaccggg ctcgtcgaat gccgtcgaac tgccgttcga gcgcggcgag
    15121 tatgcgcaac gcctgaagaa gacccgggag gccatgatgg cccgcgggat agagctgctg
    15181 atcgtcagcg acccgaccaa catggcgtgg ctaacggggt acgacggttg gtcgttctac
    15241 gtgcaccagt gtgtgctggt ggccctggag ggtgaacctg tatggtatgg acgggagcag
    15301 gatgcctacg gcgcccaacg caccgtgtac atggcgggtg accatatcgc aagctatccg
    15361 gactattatg tgcagtcgac ggaacgccat cccatggacc tcctgtgcca agaagtcgtc
    15421 gccaggcttg gctgcctgcg ctgtcggatc ggggtcgaga tggacaatta ctatttcagc
    15481 gccgcggcat acgccgcctt gcgccgcaac ctgcccgatg cccaatgggt ggatgccacg
    15541 gggctggtga actggcagag agcggtgaag tccccgcgtg agataagtta tatgcgtgtg
    15601 gcggcacgca ttgtcgagcg catgcatcag cgcattctgg aagtgatcga gcctggtatg
    15661 cgcaagaacg agcttgtggc gcaaatctac gcggcgggta ttcagggagt gccaggtcac
    15721 ggaggagact atccggccat cgtgccgctg ctgcccaccg gccgggatgc cgccgcgcca
    15781 cacctgacct ggaacgatgc gccgatggcg ggcaatgcgg gcacgttctt cgaaatcgcc
    15841 ggctgctaca aacgctatca ctgcccacag tcacgcaccg tctatctggg ccatccgccc
    15901 gcgcatttcc ttgaggctga acaagccgtg ctcgagggca tcgacgccgg tctggagcaa
    15961 gcgcggccgg gcaatacctg cgaggatgtc gccaacgcgt ttttccaacg gctgaaacgc
    16021 tacggaatcg agaagaccag tcgctgcggc tacgcgatcg gtgcgagcta ccccccggac
    16081 tggggcgaac gcaccatgag cctgcgtccc ggcgaccgca ctgtgcttga acccggcatg
    16141 accttccatt tcatgcccgg gctctggcaa aaggactggg gcctggaaat caccgagagc
    16201 atcctcatca cgcccacggg cgccgagacg ttctgcaata caccgcgcaa actgttcatc
    16261 aaagagtaag gccatgcgca attcgccgat caagcccaca gtcgacttca cgcgcgaagg
    16321 ggtgcagcat gggttcctga agctaccgta ttcacgcgat gaatccgcgt ggggagcgtt
    16381 gatgatcccg atcactgtgg tgcgtcgcgg catcggcccc actgttctat tgaccggcgg
    16441 caaccacggc gacgaatatg aggggccggt ggcgctggtc aaattggcca attcgctcaa
    16501 ggcatccagt gtgtccggtt gcgtgatcat catcccgttc ctgaattatc cggcgtttct
    16561 cgcgggtcgg cgcacttcgc cgattgatca tggcaatctg aaccgcgttt ttccagggcg
    16621 gccggatggc accgtcacag aaaagatcgc ggactacgtt gagcgctatc tgctgccaat
    16681 ggccgactat gtgctggaca tccatgcagg ggggaagacg ctggattttc ttccgcttgc
    16741 ggccattcat atactcgacg acaaacagca gcaagcgcgg tgcgaagcgg ccatgcgcgc
    16801 gtttggtgcg ccttactccc tgaaaatcct cgaactcgac ggcgtcggga tgctcgattc
    16861 ctcggcggaa caggcgggaa aggtgttcgt gtccacagag ctggggggag ggggggcggt
    16921 gacggctgcc agtatcgcgg tcgccgatgc cggtgttcat cggtttctgg tgcatgccgg
    16981 tgtctgccgc ggcaaatccc cgtcgcgtgg gacgatcatg ctcgacatgc cggacgacgc
    17041 ttgctacacg cagagcgagc atgacggcct gcttgaaatg tgcaagagcc tgggtgatca
    17101 tgtcgaggcg ggtgatgtgc tggcccgtgt ctacgacacg acccgcaccg gggtgccggg
    17161 tatcgcctat cgcgcgggct gtgacggcat tctcacagcc aggcactttc ccggtttggt
    17221 caagtgcggt gacacgattg ctgtggtggc tcgtgttgtg cccaacgctg actgacacca
    17281 ccttcccgca acaggacgcc cacctagatg gtaaaacttg atcgctacga cctgcagatt
    17341 ctcaagaccc tggcaacccg gggccgaatc accaaatcag aactggccaa gatcgtgtgt
    17401 ctgtcggtgt cgcccacttg ggagagggtc aagcgcctcg agaccatggg gctcattcgt
    17461 ggttatcgcg cggtcgtgga ttggggtagc atttttcgca gcagccaggt gattgtcgaa
    17521 gtgcgccttg accgtcacac cgcctatgac atgcagcgtt tcgaacagcg ggtcgccgaa
    17581 gcacccgagg tctcccagtg ttatgccacg ggtggtggtg tcgattattt cctgcatgtg
    17641 cgtgcccgcg atatcgatca ataccaacgc ttcatcgacg gcttgctgct cgagaacctg
    17701 gggatagatc gctatttcac ttatatcgtc acgaagatca tcaagaccgc gcaggtgatt
    17761 cccgccgaag ctgaagaacc tgtgaaggcg gcgccgaaca actctctgtg atcatgccat
    17821 gaacgcccgg agcggtctgt ggcgtggcct tgaatgagcc cctcacgcaa atattctgtg
    17881 gcagccgatg ttttcagata aaaccggggc gaggctgttt caaagcggat aaaacccgcg
    17941 cgtgcgcacc cctacactga tcattgatgg cttccgccag tatcaatccg tgtttccgtt
    18001 ggggagtggc cggcatgttt cttgcgcaac cgttgttgtt caaatcgttg tgctatataa
    18061 atgggcactg ggtccacagt gactcgggtg ccagcctggc cgtgcataat ccggctgatc
    18121 agtccgtgct gggccacgtg ccgatgctcg agaagcttca aatcgaggct tccgtgcagg
    18181 ccgcgcaacg tgcacaggca tcctggcgcc acgtcccggt caatgaacgc tcggcccatt
    18241 tgcatcgatg ggcggggctg atattggaca atcgcgacga tctggccaag atcatctcgc
    18301 ttgaacaggg caagccattt tccgaagccc tgggcgagat cgactatgcg gcaagtttca
    18361 taccctggta cgccgagcag gcccggcgac tggatgggcg cttcattccg agccacctga
    18421 ccaatgccca gccgggaacc ctcaaggaac ccgtgggcgt tgccgccttg atcacccctt
    18481 ggaattttcc ctcggccatg atcacccgca aggcagcggc agccctggca gccggctgct
    18541 cggtcgtggt caagccggcg cacgagacgc ctttctcggc tctggccttg gcgcaactgg
    18601 ccgagcaggc cgggttccaa ccaggattgt tcaacgtggt cctgggcgag ccgcagatgg
    18661 tgatgcagac actggtctcc catcctctcg tgcgcgccgt cagtttcacc ggatcgaccc
    18721 gggtagggcg tttggtcatg cgggctgtcg ccgaagcggg agtcaagcgc gtggccctgg
    18781 agcttggcgg taacgcgccg ttcatcgtaa cgccggacgc ggatcttgac ctggcggttc
    18841 gcgtggcggt ggaagccaaa ttccagacat cgggacagga ctgctgcgcg gccaatcgca
    18901 tcctggtgca tcgcgacctg tacgcgccgt ttctggatcg ctatgtgaaa aaggtgggaa
    18961 atctgcgcgt gggccccggt ctggaccccg caagcgagat tgggccgctc attcatcagg
    19021 ctgcgttcga cgccacggta cagcgtgtgg acgatgcatt gcggcgtggc gcccgactac
    19081 tcaccggtgg cttgcccgat gcgcagggtg gcctgttctt ccagcccacc gtgctggcag
    19141 acgtcgcgcc cggcatgcgc atctacgacg aagaaaactt cgcaccgatc tcgggcgtgt
    19201 cgtcgttcga cacgttggag caggtcgtcg aacttgccaa tgacacgccc tatggactcg
    19261 cggcgtacct gtgcgcgtcg cgctcggacg tgatctggcc gctgctgcgc aaactggact
    19321 tcgccatggt gtcggtcaac ggagcgcgat tcaccggagc gcccgtaccg tttggcggca
    19381 tgaaagagtc gggtctcggc cgcgagggag gtgacgaggg tttcgatcct tttgtggaaa
    19441 ccaaatattt ttgtcttagt aacctgggct tgcccgccac gggcaccccg catttcagga
    19501 gcaccctatg aaaaagaccg ctgagcaatt gctggccatc gaccgccagt atttcatgca
    19561 cccgtccacg catgcccacg atcacgaatc gggggcgttg ccgggccgta tcgtgacggg
    19621 tgcaaaaggc atacgcatca ccgatcatca caacgaaacg ttcatcgacg gcttcgcggg
    19681 gctgtattgc gtcaatattg gctacggtcg gacagaggtc gcggacgcca tgtacgaaca
    19741 ggccaagcgc cttgcttact atcacacgta tgtgggccac tccacgaatg ccatcgcgga
    19801 actgtccggc cgcatcatca gctgggcacc caagggcatg aggaaggtgt actacggcat
    19861 gtccggttcg gacgccaacg agacacaagt caagctggtc tggtattaca acaatattct
    19921 cgggcgcaag aacaagaaaa agatcatttc gcgcatgggg ggctatcatg gttcaagtat
    19981 tgcgtcgggc agcctgacgg gcctggacgc ctaccacaag aatttcgacc tgcccctcga
    20041 ccggatcaag cacaccgtgc gtccacactg gtaccgggaa gcaccagacg gcatggacga
    20101 gtccgcattc gcccagtact gtgccgacaa actggaggaa ctcattcagg cggaagggcc
    20161 ggatactgtc gcggccttca taggtgagcc cgtgctcggc acaggaggta ttgtgccgcc
    20221 accgacggga tactggcggg ctatccaggc tgttctcaag aaacacgaca ttctcttgat
    20281 tgctgacgaa gtcgtgtgtg gattcgggcg tctgggaacc aagatgggca gccatatgtt
    20341 cgacattcag ccagatctga tcactatcgc caagggtctc accagcgcat acgccccgct
    20401 gtctggggta attgtcggcg agcgcgtctg ggacgtgatt tcacggggct cggaggagca
    20461 gggggccatg gggcatggtt ggacgtattc cggccatccg gtatgcgcgg cagcggcggt
    20521 cgccaacctg aatattctgg aaaccgagaa catcatggcc aatgcggctg aagtgggtgc
    20581 ctatctcctg gacagcttgc acactacctt cgatggtcat cccatggtgg gcgaggtgcg
    20641 cggtgtgggc atgctcgcgg ctctggagtt catggccaaa cccaaagaac gcgtcgcttt
    20701 cgacgctgcc ctgaaagtcg ggccccaagt ggccgctgcg tgcatgtccc gtcacctgat
    20761 cgcgcgagcc atgccggggt atgacgtcct ggggtttgcg ccgcctttgg tcacgacgcg
    20821 tcaggacgtc gacgaaatcg tgcgtatcac gaaagaggct gttgacgagg tcgcgaacca
    20881 ggtcatgtag aaagccccga ccggctcgcg cactcgaaag gtgtaaagtt atatccgccc
    20941 cgtgcgccac gcggccgtat cacgccgcgt ggcgcatgct tttccggcgg agggggcgtg
    21001 ttttcttttt ttggtgagta cgtcagaatg agtcgcccct ttcgtttggc tatcaacggt
    21061 tacggccgaa tcggccgctg tttcctgcgt gcgttgtatg agtccccgtt gcgccacaag
    21121 ttccagattg tcgccatcaa cgagccggcc gacctggaat ccatggcgca tctaacacgt
    21181 ttcgactcta cgcacggcgt ttttcccggc aaggtggcct gtggtgacag agccctgctg
    21241 attgatgatg agcccatttc ggtcagccac tccagttccc ccgaaggggt ggattggggc
    21301 agcctgggca tcgacatgct ggtcgaatgt tctgggcact ttggcacgcg ggctgacctc
    21361 gaacgttttc ttgccgccgg gtgccccagg gtgctggtgt cgcacccggc ccgcagcgcc
    21421 gcggatgtcg atttcacggc agtctatggc accaatcatg aacaactgga cccgtcgtcg
    21481 cgtctggtgt cgaacgcctc ctgcactacg aacgccatcg tgcctgtcct ggatcagttg
    21541 catcgtgcat tcagtgtcga gcaggtattc ctgaccactc tgcattcggt gatgaacgac
    21601 cagcccatga tagacggcta tcaccattcc gatctgcgcc gcacccgttc ggcgatgcaa
    21661 tcgatcattc cggtttccac cgggctcgcc cagggggtgg agcggctttt gccgcaactg
    21721 gcgggcaggg tgcgagccaa ggccatccgc gtccccatct tgaacgtgtc cgccatcgat
    21781 ttcatggtca atctgggccg tgacgtcacg gaagacaccc tcaacgccgt gatggccgat
    21841 gcggcgcagg cccatcccag gctcattgcc gtgtctaatg gtccgcacgc atcggttgat
    21901 ttcaatcacg acgcgcgttc cgcgatcatc gatgcgagcc agacgcgctg caacggcccg
    21961 cgtttcgcga atttgctcgt ctggttcgat aatgaatggg gcttcgccaa ccgcatgctc
    22021 gacatggcag gcttctgggc cagtcgcctg cgattactga taaaggacac accatgacct
    22081 ctacccgagt cgaaacggat tccatgggcc agatcgacgt cccggccgat cactactggg
    22141 gggcgcaaac gcaacgctcc atactgaatt tccccatagg tgtcgcgcgc ttcaaatggc
    22201 agcgccctgt cattgcggcg ctgggaaccc tgaagaaggc ggccgcgcag gccaatgccg
    22261 aactgggtga actaccgccg gatctcgccc ggttgatcgg gctggcggcc gatgaggtca
    22321 tagaaggccg gctcgatggc gagtttccgc tggtggtgtt ccagacaggc tcggggaccc
    22381 agtccaacat gaatgccaac gaagtcattt ccaaccgggc catcgagctg gcaggtggca
    22441 tcatgggcag caagtccccc gtgcatccga atgatcatgt caataggggg cagtcctcca
    22501 acgacacgtt tccgaccgcc atgcacatcg cggtggccca ggaacttgca cacgcgctgt
    22561 ttccgtcggt gggcgcattg cgaaaaacgc tggcggccaa gtccgagaaa ttccagagcg
    22621 tggtcaagac ggggcgcacg catttacagg atgccacccc catcaccgtg ggtcaggaaa
    22681 tcagtggctg ggtggcacaa ctggatttcg ccctggatgc cgtgcaggcg gcgttgccgg
    22741 gcatctacga tctggccatc ggtggcacgg ccgtcggcac gggcctcaat gcgcatccgc
    22801 gtttcggtga agaggctgcg cggcacatca ccgatatcac tggcctgcct ttccggtcct
    22861 cgcccaacaa gttcttttcg ttgtccgcgc atgacgcatt ggtcaacctg tcggcttccc
    22921 tgcgcacgct ggctggcgcg ctcatgaaaa tcgccaatga cgtgcgctgg cttgccagcg
    22981 gtccgcgttg tggcatcggc gaactggtca tacctgacaa cgagccgggt tcgtccatca
    23041 tgccggggaa agtgaatccg acacaatgcg aagccatgac gatggtgtgc gtgcaggtgt
    23101 tcggcaacga cgccgccgtg gccttcgccg gtagccaggg caacttccaa ctgaacgtgt
    23161 tcaagccggt gatggtgcac aacgtgctgg aaagcatcgg actattgagt gatgcctgcg
    23221 catcgttcaa cgaccattgt gcggtgggca tagagcctaa ccttgaacgc attgccgaac
    23281 acctggagga gaacctgatg ctggtcaccg cgctgaaccg gcacatcggc tatgacaagg
    23341 ccgccttcat cgcgaaaagg gcgcacaagg aaaaatccac attgcgcgcc gcggcacttg
    23401 cctcaggcta tctcacggcc gagcaatacg atgcctggat cgtgccgctc gacatgacgc
    23461 accccggctg atcgggcacg ccgcttgcgc gggtgggctg attacctgcc ggtcgatttt
    23521 tccgctttca cgcgggcatg gccttcaact gcttcttggt atatcccttg atgagcagtt
    23581 tgtcgccgtt cttcacaatt ttttcatacg gaatcgctat atcgtgttcc cctggtccaa
    23641 ggaagccacc gaccccaatg gcgacacgca ccgcttttcc gccattcccg gtatcacagc
    23701 atcaacgtcg cccattttca tgccttcacc gttgtaaacc gtgcttccga gagctccgtc
    23761 cttcgcgctc cagccggtta ctttttgagt tgattcaggt gccggggcgg ctgcgccacc
    23821 cggtggcggt gccatggcgc cgaaggagcc gctcctgttt acgcatatac tgtggacgcc
    23881 agtgtcaacg gcagcgtgca ggctatgttg tgatcatttt tttcatcatg agccccttgc
    23941 aaatgaccgg gcggagcagg ctgttgccgc ccgcggtcac gccgttggca gcaatttccg
    24001 tgccacgcgc cgcttccggg agccgggcac gcgatttgcc ggtacccgag caaccgtgac
    24061 caacgaacga cgccgccgcg tcaggttatt tgtagaagag gcatagcaat gaaaacaact
    24121 cagcgaatcg cgtattgcac tatgatattg gcgatgtcgg ccagcctgtc cgcctgcagc
    24181 ggcatgtctc atcgtggaca gagtacggcg atcggggccg gggttggggc tctgggcggg
    24241 gccatcctta ccggaggcag cgcactcgga accgtgggcg gcgccgccgt gggtggcgtg
    24301 atcggcaaca tcgccggcca acggtagaaa tttcgaatcg ttttcggcgc ggcgtgcgtg
    24361 cgcgcgccgg ccagtgcttt ctgcgtccag ccgggtgaca acccggctgg acgcagtatt
    24421 tggagacggc ccgccgtgcc gagatgatcc cttcggcagc cagtcgatcc accagttccg
    24481 cgtcgctcag atgagaagcc ggagggcgat tcaatcagat catgaatcag tgcgcgcccc
    24541 gctgtcataa tcaaagaaca actgttgtcg cgccacgatt ggctggccat cgtcggactc
    24601 gtcgcattct gagcaggcgg gtgactcgct tgtaaaagcg tcatgatcgg agtacttttt
    24661 gaacgaaggg ccgcgacatg gccgcgacgt atatcggttt gaactttcga tgtcggggcc
    24721 agtctaccca taaggcgttg tccggggccg cggtaggtgc gcggctcatg aatgccgttg
    24781 cttccagcct gtcgcaggca agaaaaccgg gcgatccaag tcaccactgt caggagatcg
    24841 catatgcatc catcccgtcc ttccacacct gacagcaacg tttccagtgg tggggcggac
    24901 agaaagacgg aatgtgccgg cactggcaac caaagggtct cttcggtgaa gctgctgtcc
    24961 aacggccggt atcacgtcat gctttcggcc gctggcggcg gatacagtcg gcacgggcag
    25021 aatgccctga cccgttggcg ggaggatgcg acgcgggact gctggggcgc gtttctttat
    25081 gtgagggacc gtggcaacgg agacgcatgg tctccagccc ggcaacccat ggtcgatgcg
    25141 gcggtggaac gcagcatcga tttttccggt tccagtgcgg catttattgg cacgacctcc
    25201 gacatcacga cccgtgtcga cgtcacggtt tcgcctgatg ccgatctcga ggtgcgcagg
    25261 gttcgtgtca ccaatcactc gtcgcgcatc cgttctctcg atctcacgag ttacgctgaa
    25321 gtcgtcctgt cgtccgcgcc ggccgatgat gcgcatcccg cgttcaacaa gctgttcgtg
    25381 cagacagata tcgacgaatc cgtgtccgcc attctgtgcc acaggcggcc tggcaatggc
    25441 ggtgatccgg cgccatggtt gtttcataca gtggtcgtcg acagcccggg aattgccaac
    25501 ctgtcctatg aaaccgaccg tgcccgtttc atcggtcgtg gccggacgct ggcagacccc
    25561 gctgccgggc aggtcgaatc attgtccggg agccaggggt cggttctcga ccccgttctg
    25621 tccattcggt gtcctgtgcg acttgaggcg gggcagtccg ccaccgtcag ttttctgtat
    25681 gggatcgccg agaatcgggc tgcctgtgaa gcgttgataa aaggcttcca gagccaagtg
    25741 aaagtggacg agacttttga tgacgcggct ggtgcacgca agaagaccct ggccaaactg
    25801 aatttgaatg actccgcggc ggccctttac gaccgactgg ctgcccgggt cgtttatggc
    25861 ggcgcgcaat tgcgcgccga tccgtcgatc atcctggcca acaggcaagg ccagtccggt
    25921 ttgtggggtt tttccgtttc gggcgacttg ccgatagtgt tcctgcgggt cggcgcctcc
    25981 ccggatatgg ggaaagtgcg ggaactggtg caggcacacg cctactggcg attcatgggg
    26041 ctggcggctg atctggtcat cgccaacgcc gatggcatcg agcctcgcca gggtgttcag
    26101 caacagatac tggatctcgt caagaccttg gagcagtctg atcagctgga ccagcgtggc
    26161 ggcattttcg ttcgcctcgc cacgcggttc agccccgaag accgggtgct gtttttgtcc
    26221 gtggcgcgcg tcgtgcttga cgatggtcca ttggggctgg aggacagact cattgcggtc
    26281 gaaggtaaca ccgcacgccc aatgcatgct aagacgccgg ccagcattgc atcgatgcct
    26341 gccagatcac ccgccgccga atcgtcgagt cccgaccgca tcattgataa tgggataggg
    26401 gggttcaccc cggacggccg tgaatatgtc atgacgctcg acgagggcgc catgacaccc
    26461 acgccctgga ccaatgtgct ggccaatccg gatttcggca ccattgtgtc ggaaagcggg
    26521 tgcgccaaca cctggagtga aaacgcgcat gaattccgcc tgacgccttg gtccaacgac
    26581 ccggtttgcg actcgtccgg cgaagcggtt tacctgcgcg acgaaacgtc cggcgcgtac
    26641 tggtcaccca caccgtggcc ttgccgggac cagggtgcgt atgtttgccg gcatggattt
    26701 ggctacagtt ccttccagca tgttcatgac ggcgtggaat cggagcttat tgtctatgtt
    26761 tcacgcagcc agccggtcaa gttcacctct ctcaaagtcc gtaaccagtc cggccgccgg
    26821 cgccgccttt cggccaccgg ctatgtggaa tgggtgctgg gagaccaacg gatcaagacc
    26881 atggcgcagg tcaccacacg tatgaatccg agcggccgcg ccatcgtggc gcataacgcc
    26941 tacagcatgg aattcgggga ccgccacgcc tttttttccg tggatgcgga tccgggttcg
    27001 gtgacggcgg atcgtacgga gttcctgggt tgctgcggtc gggcagagca gccagtcgcg
    27061 atggcgcgtt ccgccttgat gggggccgtt ggtgccgcgc tcgacccttg cgccgcactg
    27121 cgcgtgccgt tcgaactcgc tccaggggaa gagcgtgaca tcacattcat gctcggcgct
    27181 ggcggcaacg aggccgccac gcttggcctg gtcgaacaat gcggcagggc gggcgccgcc
    27241 caggcggccc tggacgaagt gcaaggctat tggcgccaca cgctcgacac tgtccgggtc
    27301 aagacacccg atcccgcctt caacatgcaa gtcaacggct ggttggccta ccaggccctg
    27361 gcgtgccggg tctgggcgcg cagcgcgttc taccagccgg gtggcgcctt cggctttcgc
    27421 gatcaactgc aggacgtcat ggcgttggtc cacgcggagc cctccctggt gcgggcgcac
    27481 attctgctga gcgcctcgcg gcaatatccc gagggtgacg tgcaacactg gtggcatcct
    27541 cctgcgggac gaggggtgcg cacccgctgt tgcgatgact atctctggct ggctcttgcg
    27601 acatgccgct atatcaccgt cacaggggat gccggcatat tagatgaacc cgtgcttttc
    27661 cttgacggcc gtccgctcaa gccggacgag gattcctatt acgagttgcc cggggtgtcg
    27721 aaagaaccgg cgcctcttta cgaacattgc gcgcgcgcga tccgccatgg gttccgcatg
    27781 ggaatccatg gattcccctt gatgggcgcg ggcgactgga acgacggcat gaacaaggtc
    27841 ggcgcccagg gcaaggggga aagcgtatgg atggctttct ttctgtatca catcctgcgg
    27901 cagttcaagc cgttggcaca gtcacgcgac gaccaggagt tcgtgaaaga gtgtgacgcc
    27961 gtcgcggccg gcctggccga cagcctggac aaaaacggct gggacggcga atggttcatc
    28021 cgcgcgtatt tcgacgacgg ctcgcctttg ggctcttcga agaacacgga atgccggatg
    28081 ggttccattg cgcagagctg gtcagtcctg tcaggcgccg ccgcgccaga gcgagcccac
    28141 caggcgatgg attcgctcga ccgaatgctg gttgaccgcg acaacgccat catcaaactg
    28201 ttgtcgcctc cgtttgatgt ctcggtcccc gatccgggct atatccgggc ctatgtgccg
    28261 ggggtgcggg agaacggcgg tcagtacacc catgccgcgg tatgggcggc gatggctttt
    28321 gctgaattgc acgacactcg ccgggccgcc gagctcatcg ccatcctcaa tccggtcaac
    28381 catgcattgg acgccactgc ggtggaaaga tataaagtag agccgtacgt cgtcgcctcg
    28441 gatgtctacg cactgccccc gcacaccggg cgcggtggct ggacctggta ttcggggtcg
    28501 gcgggatggc tgtaccgctt gctgctggag tcggtcgtgg gtgtgcggcg cgaagccgac
    28561 aagctgtttc tacggccgtg cgtgccggat cactgggacg cattcagtgt cgactaccgt
    28621 tatggcgcca ccgtctatca tatagatgtg cggcgcgaag ccaaggtcgc gggttccgcc
    28681 agcccggtca ttgacgcgga cagcctgcag agtccggtga ttccgctggt cgacgatggc
    28741 ggcgagcacc ggatcacgtt caccttgccc gcttcaccga atagggattc agccaaggtg
    28801 acgcattgat tattcacgtc cgcaagcatc ggaaacaagg gagattcaaa caatgcagat
    28861 aggcatggta gggctggggc gcatgggcac caacatggcg cagcgtctca tggcgaaggg
    28921 cgtggattgc gtggtgttcg acacccatca gcaggctgtc tcggctttgt cgaccgaagg
    28981 cgccgcgggg gcggcgtcgc tgcgggaact ggtcgggaaa ttggcgcaac ccagagccgt
    29041 gtggctgatg gtgcccgccg gggtggtcga tggcgtgctc gatcagttgg ttcctttgct
    29101 gagcaaggat gacatcgtca tagacggtgg gaactcctat taccacgatg atatccgtcg
    29161 cgccgggaag cttgctgaac aaggcattca ttatctcgac gtgggcacca gtggcggtgt
    29221 tttcggcaag gagcgcggct actgcctgat gatcgggggt gacgacgcct cggtcaagca
    29281 tcttgacccg gtgttcgccg cgctggcccc cggccgggac accgcgccgc gcacgccggg
    29341 ccgcaatgat ggcgaaggca ccgccgagca gggatacctg cattgcgggc cgcatggcgc
    29401 cggccacttc gtgaagatgg tgcacaacgg catcgagtac gggatcatgc aggcctacgc
    29461 cgaaggtctc aatgtgttgt gcaatgccaa tgcgggcaag cgcaatcgtg aaaccgacgc
    29521 cgagaccacg cccatgcgca accctgagta ctaccagtac gacctgaatc tgcccgaggt
    29581 cgccgaagtc tggcggcgcg gcagtgtcat cagttcgtgg ctgctcgacc ttaccgcgca
    29641 tgcgctgcat ggtaatccgg atcttggaaa ttttgcgggc cgtgtctcgg attcgggcga
    29701 ggggcgctgg accatccatg ccgccatcga cgaaggcgtt cccgtgccgg tgatcagtgc
    29761 cgcgctgttt ggtcgcttcc agtctcgcgg caacgccgat ttcgcggaca aagtgctgtc
    29821 ggccatgcgc aaggaattcg gtggccatga cgaaaagaat cggggggcct gacatggtcc
    29881 aacccgcgaa gaaggttcaa ccatccgatg cactggtcgt gttcggtatc acgggcgatc
    29941 tggcgcacaa gaaaatattc cccgcgttgt acgagatggt caaacgcgac ggcctgaacg
    30001 taccggtggt tggcgtggcc tcgtcgagca tgaccctcgc ccagttgcgc aagcgcgccg
    30061 cagacagcat tcgcgaggct ggcaagatcg atgacaagga agcattggaa cgattgcacg
    30121 ggttgttgaa atacgttcag ggcaattaca acgacgaggc gaccttccat acgctcaggc
    30181 acgccctggg caaggcgcga cgcgtgtcgt attacttggc gattcctccg tctctgttcg
    30241 gaacggtgat acaagggctc caccacgccg gtctggccga agacggccgt gtcattgtcg
    30301 agaagccatt cgggcgtgac ttggcgtcgg cgcgtgaact gaaccgggtg gcccactctg
    30361 ttttcccgga agagtccatc ttccgcattg atcacttcct gggcaaggag gcgatcatga
    30421 acattctgta cttccggttc gccaactcgt tcctggagcc catctggaac cgtgatcacg
    30481 tggaaagcgt gcagatcacg ttatccgagc aattcggcat cggacaacgt ggcgggttct
    30541 acgagactgc cggttgcctg cgcgacgtca tccagaacca tctgttccag atcgtggcgc
    30601 tgttggccat ggaaccgccg gtccatcaag attacgcagc catccatacc gaaaaatcca
    30661 aggtctttga ggccatgcgg ccgcttggca ttgacgatgt cgtgcgtggc cagtacacgg
    30721 ggtaccgcaa agagccccat gttgcccgca catccgatgt ggagaccttc tgcgccgtgc
    30781 gattgttcat agattcgtgg cgctgggcgg gcgtgccctg gtatctgcgt tccgggaaat
    30841 gcatggccga gacggcggcc gaggtgctgg tccagttgcg cccgccaccg caacgattgt
    30901 tcgccgactc gatgtctgaa cggcaaagat ccaactacct acggttcagg ttgtcaccca
    30961 actcggctgt ggcgctggca gcgcggatca agcgttcggg caaggaattc gtcggcgatc
    31021 agcgcgaatt gttcctgctt gacgccgacc ccaaccagga gacgccatac gaacgtcttc
    31081 tgacagatgc actggccggt gacagttcgt tattcaccag cgaggacgcc gtcgaagccg
    31141 cctgggcggt ggtggacccg attctttccc ggcaccacaa ggccatttcc tatcgacgcg
    31201 gcagttgggg gcctaaagaa gccgacgcct tgatcgcgcc tcatggcgcg tggctgaacc
    31261 ctcgcctcga ggagcgcgca tgacaacgcc gcgggaagaa accgtctttc tgttcgatgt
    31321 cgacaacaca ttgctcgaca acgatcgggt caccgacgac ctgcgccggc atctgatcga
    31381 gcagtttggc cgcaaaagcg cggagcgcta ctgggccctg ttcgaggcgt tgcgcactga
    31441 actcggctac gccgactatc tgggggcgtt gcaacgttat cgactgggtt tggtaaacga
    31501 ccccgctttg ctgaaaatgt cgtcctttct actggaatac ccttttgcgg aacgcttgta
    31561 cccacgtgtg ttcgatgtgc tggagcaagc cggaaaatgg ggaacgaccg tcattctgtc
    31621 tgatggtgat gcagtgttcc aaccccgcaa agtcgagcgc tcggggttgt gggacgctgt
    31681 ggaggggcgg gtgctcatct acatccacaa agaaaagatg atcgatgccg tgatggaacg
    31741 ctatcccgcc agccggtatg tcatggtgga tgacaagctg cgcatcctga cagccatgaa
    31801 aaaacgaatg ggtgccagac tgctgactgt gtttccgcgc caaggtcatt atgcacacga
    31861 cttgaagacc gtgcggttgt tcccgccggc ggatattact gtggaacata tcggggatct
    31921 gctcgacgtc gatctaccgg cccatttgcg ggcgtcagac gacgaagcaa cgacaaaaga
    31981 ggttacatca tgaatgctac acgtcaactg cacgatctgg ggcaaagcct ctggttggac
    32041 aacatcaccc gcggacttct cgacgacggg acactcaagc actatctcga tgatctggcg
    32101 gtgaccgggc tcacgtccaa tccaaccatc tttcagaaag cgattcaggg aacgacgttc
    32161 tacgacgagg ccatcgcgca caaggcacag gccggcaagg cgggggaaga cttgttcttc
    32221 gagttggcca ttgaagacct gacgcgcgcc gccgacttgt tccggccggt gcacgacgcc
    32281 acccggggca tggatggttg ggtgtcgctg gaggtgtcgc cgttgctcgc caatgacacc
    32341 gacgcaacca tccacgctgc aaccacgttg catgcgcgcg ccaaccggcc gaatctgttc
    32401 atcaagattc ctggtacgct cgccgggctc aaggcgatcg aggaatccat tttcaacggt
    32461 gtacccatca acgtgacgct tctgttttcc tgcgagcagt acctggcggc ggccggcgcc
    32521 tatttaaggg gcattgagcg ccgtgtgcag gcgggtttgt cgtccgatgt gcgcagcgtg
    32581 gcctcgctgt tcgtgagccg ctgggacgtg gccgtgcaga gcaaggtcga tgcggggctg
    32641 cacaaccggc tgggcgtcgc cgttgcgtgt gaaacctttg cggcataccg cgcgcttctc
    32701 gcgtcgccgc gctggaagaa actggaagcg gccggggcac ggccgcagcg gctgctgtgg
    32761 gccagcaccg gcaccaagga tcccagcgcg tccgacacgc tctacatcga tggattggcc
    32821 gccgcgggca ccatcaacac catgcccgag aaaaccttgc tggcattcgc cgatcatggg
    32881 aacccgggcg caccgctgcg ggacgagcag ccggcggcca tcgccgagct cgcccagttc
    32941 cgggcggccg ggatcgatct cggcgcgctc gcgacgcgcc tgcaggaaga gggcacggac
    33001 gcgtttgatc agtcgtggca cgcgcttctg gatggcatca acgccaagtc ggcggctctc
    33061 aagagcgctt ccacccattc caggacctga cgcgatgacc cctccaagca cgccaaaact
    33121 gcagcctttg acccaatccg ttggctggaa agccttgacc gcccattgtt ctgtggccga
    33181 aggattgcac ctgcggcggc ttttcgccgg tgatccggag cgtgggcagc ggctggctct
    33241 cgaggccgcg ggcgtttatt tcgattattc gaaacagcgc gtgaccgacg aaaccattcg
    33301 tcttttgctg gcgctggcgc gggagcgcgg ggtagaggaa aggcggcgcg ccatgttcgc
    33361 cggcgaggca atcaacgcca ccgagcgccg cgccgtgttg cacgtggcct tgcgcatgcc
    33421 ggggactgaa cagattcggg tggatggcca cgacgtggtg ccggatgtgc acgaggtcct
    33481 gtcccgcatg ggcactttcg cgcagaaagt gcgcgacgga aaatggctgg gttataccgg
    33541 caagcccgtg cgcaacatcg tcaacatcgg cattggcggc tcctcgctgg gtcccgtcat
    33601 ggcctaccag gcgctaaaac actacagccc gcgcgatttc acattccact tcgtgtccaa
    33661 catggattcg accgatctgg tggaagccgt gcgggatctc gatgccgaag agacgctgtt
    33721 catcatctgt tcgaaaacgt ttggcacgct ggaaaccctg accaacgccc acgctgcccg
    33781 tcaatggtgc ctggaccggc tcaaggacga acgcgcttta ccgcgccatt tcgtcgcggt
    33841 gtccacgaac gccgaacggg tcgcgaaatt cggcatcgac accgccaaca tgttcggctt
    33901 ctgggactgg gtcggtgggc gttactccat ggattcggcc atcggcctgt cgaccatgat
    33961 cgccatcggc cccgacgggt tctcggatct gttgtccggc ttccacgcca tggatgtcca
    34021 ctttcgcgac acaccgcttg agcgcaacat gccggtgctg atggggttgc tgggtgtgtg
    34081 gaacaccaat ttcctgggtg ccgagaccgt ggcggtgctg ccgtacgatc aatacctgtc
    34141 gcgctttccg gcgtatctgc agcagctcac catggagagc aacggcaaat ctgtcgtcac
    34201 cgacggcgcc agccaggtga accaccagac cggccccatc tactggggtg aacccggcac
    34261 cgatggccag cactcgttct accagcttat ccaccagggc acgcatgtga ttccctgcga
    34321 tttcatcgga ttctgccaag gcctgaaccc gctcggcgat cagcacgacc tactgatgtc
    34381 caacctgttc gcgcaaaccg aggcgctggc ttttggaaag actcacgaac aggttctcgc
    34441 cgaaggcgtc ccggcatggc aggctcccta tcgcgtgttc gacgggaatc ggccgaccag
    34501 caccttgctg ctcgagcgcc tgacgcccca tgccctgggc acgctggtgg cgttgtacga
    34561 gcacagcgtc ttcacacaag gggtgatctg gggtatcgat tcgttcgacc agtggggggt
    34621 tgagttgggt aaggccttgg cgctgaaggt gtcgcccgag ctgaccagcc ccgatgaacc
    34681 cgcgctggcc cacgacagct ccaccaacac actgatacgt cgttacaggc ggctcaaggc
    34741 gggccgcact tgattcccgc gattctggag ggtccatggc acccaatacc gaattagatc
    34801 aactttgcat caacaccctt cgcacactgt ccatcgacgc ggtgcagaag gcccagtccg
    34861 ggcacccagg cacgccaatg ggcgccgccc cgatcgctta cagcctgtgg cagggttttc
    34921 tgcgctacag tcccacgcat cctgactggc tcgaccgcga ccgtttcgtg ttgtcatcgg
    34981 gtcatgcctg cgcgctgctg tacagcctgc tgcatctcgc cggcgtgcgc gcgcacctgc
    35041 ctagctacgg cgacactgac cggctggccg tttctctcga cgatctgaag cgattccggc
    35101 aactcgacag ccactgcacc gggcatccgg agtacggttg gacgaccggg gtcgaagtga
    35161 ccaccggccc gttggggcag ggggtggcca acagtgtggg catggccatc gcggggctgt
    35221 ggatggcgca gactttcaat cgacccgggt tcgaactttt ccggcacaac gtctacgcat
    35281 tgtgcggcga cggtgacatg atggagggca tcagcgggga agccgcctca atggcgggcc
    35341 acctcgggct gtcgaatctg tgctggatat atgacgacaa ccacatctcc attgaaggca
    35401 acacgcgtct ggctttcacc gaagatgtcg gtgcgcgctt cgcggcatac ggctggcggg
    35461 tcgtgcgggt gggcgacgcc aatgatctgg acgctctcaa cgacgctttt caagtgtttc
    35521 tctcgaccac ggatcgcccc acgctcatca tcgtgcgcag tcacatcggg tacggagcgc
    35581 cgcacaagca agatacgcgc gaggcgcacg gcgagccgct tggcgccgat gaagcgcggc
    35641 tcgcgaagga attctatggg tgggatcccg acgcgcagtt tcacgtgccg tccgaggttc
    35701 cggaacattt cgcgcgccat ctcggacagc gtggcgaggc gatccgcgcc gcctggcaag
    35761 agacatacca ggcgtacgga aagcaatatc ccgacctggc tcgacagatc gaactcctgc
    35821 gcaaccgcgc gctgcccgaa ggatgggatg ccgccttgcc cgtgtttccc gccgatgcgc
    35881 gcggcatggc gacccgggct tcgtcaggca aggttctcaa cgccatcgcg ggcaagatac
    35941 cttggctgat cggtggagcg gccgacttgt cgccgtcaac caagacggag ctcaccgacc
    36001 cgctttatgc gagtttccag gcccctgcgt tcgatggaaa ccatgggggc cgcaatttcc
    36061 acttcggcat acgtgaacac gccatgtgcg cgatcgccaa tggcatcgtg gtgtccggtc
    36121 cccgtccgtt cgcctccagc ttctttgttt tcaccgatta ttgccgtccc tcgctgcgcc
    36181 tgagcgccat gatgggcgtg ccggttatct acatctggac gcacgactcc atcagcctgg
    36241 gcgaggacgg tcccacgcac cagcccatcg agcatctgcc gtcgctgcga gccatgccgg
    36301 gcatgatcgt gatccgcccg gccgatgcca acgaggtcgt ggaggcttgg cgggcgatca
    36361 tgcctttgcg cgaccatccc gtgtcgctgg tgctgacgcg ccagtcactg cccacgatgg
    36421 atcgcacgcg gtatgcgccg gccgccgggg tggcgcgtgg cgcctatgtg ctggccgacg
    36481 caccggatgg gaagcccctg gtgttgctca tggccagcgg cagcgaggtg gccttgtgcg
    36541 tacaggctta tgagcagttg cgggaacaag gggtgggcgc ccgagtcgtc agcatgccgt
    36601 cctgggaact gttcgaagct caggatcagg cctaccgcga tcaagttctg cccccgtcgg
    36661 tgaccgcgcg cgtggcggtc gaggaagcgt cgggctttgg ttgggaacgc tacacgggcc
    36721 tgggcggtgc cattttaggc atgacaagct tcgggctgtc ggctccgggt ggtgatgtgt
    36781 cgcgccattt cgggttcgac tgtgagcacg tggtggctgc ggcgcaggcc cagatggcgt
    36841 cgcgaaagtc ataaacgcca cggatgtcct ggacgccgcg gatgatcaac aggctgaatc
    36901 ctgtgggcgg atggatgagg agttgcctcg aggcggttgc tccggcccac ggatggtaac
    36961 aacaagacaa gaggtgaatg catggcccga gccaaaaaaa acgaatcaat gaagatcagc
    37021 ccgttggccg gaaagcccgc cccgcgcgcc attctggtgg atgtgcccag gctgatttcc
    37081 agctattaca ccgggcggcc ggatccaggc gtgacgggcc agcgggtggc tttcggcacg
    37141 tcgggacatc gcggctcgtc gctggaactc acgttcaacg aggcacatgt cctcgccatc
    37201 agccaagccg tgtgcgatta ccggaagaag cagggtgtcg atggcccgtt gttcattggt
    37261 gccgatacgc acgccttgtc ggtacccgcc tcgcgctcgg cgctggaggt ttttgccgcc
    37321 aatggcgtgc atgccatgat atccacgaat gacgagacaa cgcctacgcc ggcggtatcg
    37381 ctggccatcc tggcgcacaa caagggacgt agacagcatc tggccgacgg catcgtcatc
    37441 acgccgtcgc ataacccgcc cgacaatggc ggttataaat acaacccaag caacggtggg
    37501 ccagccgacg tcaaggttac gggctggatc cagaatcgcg ccaacaagtt gctcgaggcg
    37561 ggcctgaagg gcgtgaaacg cagtccttac gacaaggcgc tcaccgcttc caccacccgg
    37621 cgctacgact tcctgggcgc ctatgtgacc gccttgcgtt cggtcgtcga catggatgtc
    37681 ctgaaatccg ccaccctgca tatgggcgtt gacccgctgg gtggcgccgg cgtgcattac
    37741 tgggcccaca tcgccgaccg gtacaagctg aacctggaca tcgtcagcga acaggtcgag
    37801 cctgatttcc gcttcatgac cgtggactgg gatgggaaga tccgcatgga cccctcgtca
    37861 ccgtacgcca tgaagcgcct gattcgtctg aaaagccagt tcgacattgc gttctgttgt
    37921 gacaccgact acgaccgcca cggtgtcgtc acgcgcagcg tcgggctgct cccacccaat
    37981 cattatcttg cagtgctggt cgattatctc tttagtcatc gctctcgttg gggcaagcgg
    38041 gccgcggtgg gaaaaacgct ggtcagcagc cagatcatag atcgcgtcgc agcgcggctg
    38101 ggacgcgggc tggttgaggt gcctgtcggt ttcaaatggt tcgtggacgg gctgttcgat
    38161 ggttccctgg ggttcggcgg ggaagaaagc gcgggcacgt cattcctgcg cagggatggc
    38221 acgacctgga ccacggacaa ggatggcatg atcgcggcac tgctgtcggc cgagatgaca
    38281 gcgcgcaccg ggatcgatcc gggcgagcat tacaagcgcc tggcccacga tctgggcgaa
    38341 cccgtggaca cccgtttcga ggcgccggcg acgccagaac agaagcaacg gctggcggcg
    38401 atttcggctc gcgacgtcca catcacggat cttgccggtg agaaggtcga cgccatcctg
    38461 acgcgcgcgc cgggcaacaa cgcacccata ggcggcgtca aagtcgtatc caggaatggg
    38521 tggttcgcgg cgcgcccttc gggcacggag aacatctaca agatctacgc ggagagtttc
    38581 ctgggcgaga atcatgtcca gcggctggtg gctcaagccc acgacgtggt ggacaaggcg
    38641 cttggcgccg gcgccaggtc gcagaccgca tcgcgacagg gcgggcgccg agccgggaag
    38701 gacaaggcat gagtcgtggc gccagccggt ctggcgatga gatcgcccgc agaaaatcaa
    38761 gcggcgaagc cgagctagaa ctattgcagc accggtcgtt cagttatttt ctgcacgaga
    38821 gcaatcaatc gaacggcctg gtgctggaca agagcgcacc gggctggccc gccagcattg
    38881 ccgccacggg cttggccatg acctgctacc cggtggctgt tgagcgtcgc ctgatgtcga
    38941 aggctggcgc ggtggaacgc actctggccg cgctgcgttt cttctggaac agccatcagg
    39001 ggccggaacc cgacgccacg ggccacaacg ggttgtatta ccactttctg gacctgaaaa
    39061 ccgggcggcg cgcctggcag tgtgaactct ccacagtcga tactgcgttt ttgctggctg
    39121 gggcgctggc cgcggcacag tattttcagg aaaacactcg ggcgcaggct gagatacggg
    39181 agttggccga tgcgttatac cggcgcgccg actgggcttg ggcgcagaac ggcggtgaaa
    39241 ccctgacgca cggctggcgg cccgagacgg gtttcattcc atatcgctgg gagggttacg
    39301 acgaagccct gctgctttac acactggcgt tgggctcacc cacgcatccg ctcaccgatg
    39361 cgagctatgg aacctgggct gaaacctatc gctgggattc cagctacggc tatgactacg
    39421 tttatgccgg gccattgttc acgcaccagt tatcgcattg ctggattgat tttcgtggca
    39481 tccaggatgc gttcatgcga gggaaaggca tcgattactt cgaaaacagc cggcgcgcca
    39541 cgcacgtgca atatgaatat gcgatggcga acccacacaa attcacgcgg tatggggagt
    39601 tctgctgggg cgtgacggcc agcgacgggc caggcccctg cacgcgcaag atcaacggcg
    39661 tgacccggga attcttcgat tatgtgggcc gtggcgtccc atatgggccg gatgacggga
    39721 cggtcgcccc ctgggtggtg gtggcgtctc taccgttcgc gcctgatatc gtgttgccgg
    39781 ccattgatca ctatgtcaac gaattgaagt tgtcagcgcg gaacctgtac ggtttcaaag
    39841 ccaccttcaa tcccaccttc gcgacgcccg agaccgagcc gtatggctgg gtgtcaccct
    39901 ggcatttcgg catcaaccaa gggcccatcg tgctgatgat agagaattac cgcacgggca
    39961 tgttgtggga tttgatgcgt acctgcccgc ccatcgtgaa cgggctgcgt cgagccggtt
    40021 tcacaggcgg atggctggag gaatcggcgg cgtgaagggc gtcggcgcgt cagttgcccc
    40081 gcgcggacgg gtatagtgtt cgggcccgct ggcgcgagca tcgcacatcg gcgaccaggg
    40141 ttgcgaaaaa ttgcgtgatc gtcgtggcac gagatggcac ttgggcgggg gaggcgattt
    40201 caatcacgcc gatcgcatcg gtggccggag cgaggggaga atgggttgcc ggaccaagaa
    40261 tggggtggca ggatagggtt gacatggggc tgcgttcctt gcatggacag cggatggttc
    40321 atgattaaat tataggggtt aaccctttat tctgatatcc tgtttttttc tgaaatcctg
    40381 attatctaac gaaaaaacaa cggactaccg ctttgcggca aaattttttg attttgtcat
    40441 ctaatagtca agaccgaacg ttctgcatgc catcgtaggg cacgtcaatg tcggacgggt
    40501 cttggcggca aggtggccgc agggcccggg tgcgatctgg gcatggctac aatagcccca
    40561 tgttttcgtc gcgacattct tcatgaaaca cctgcacgcc ggccactcca tcgaaaccgt
    40621 atccgtccgc gtcagcggga aagtccaagg agttggcttt cgagccgcca ccgtgcgcca
    40681 ggcgcacttg ctgggtattg ccggctgggt gcgcaatgcg tcggattctt ccgtcgaggc
    40741 tgtgctgcag gggtcggtcg atcagatcga tcgcatgctc tcgtggttga gtgtggggcc
    40801 tccggcagcc cgggtggatc aggtcgagca ccagcaggaa ttcaccattc gccattttga
    40861 ccatttcgag cagttgtgaa agtcgctatt gaaaggacac tgcatggcgt cgatattcgg
    40921 atgatttatt caccggatct ccaactgagg gcactggtgt caagctgagc ccactggtgt
    40981 caagctgagc gataaggagc caggggcagt ttgtccaact tggaccacaa cgcccggttc
    41041 tgttcctggt caggggcaag gcgcaagcct gcctcgtcat tgagtgcgac cgcgggcatg
    41101 ccggtaagct gtctcaggga aaatcgccgg gtcgtcgcgc tggcgccttc ctcgagccag
    41161 tccgcgttga ggactttcac gctgaggccc tcggcatcga agcgttcgaa aatgggcatg
    41221 ccacccagcg aacgggtgcc gacgcgcagc atgccatagg ctggatccag aagcagcagg
    41281 cgccactggc gcttgccgtc gggcagatga agcaaacggc aaagcgcaag aaggcggccg
    41341 tcttctgacc acgacatggg cggcgccaag tcgccgacgg cgcaccccga tgcggtcagc
    41401 aggtaaccgc cacttcgtgg ttcgcccgca ggtggccacc cgaccccata cgatacgccg
    41461 ccgccgaaga accaggcggc gtcggcgccg ttcgggcaag gatagatgaa atcgccatcg
    41521 ccataggctg cctgggcctg ggtcaccgtg cgctcgcgtg gcagtgccac gatgacatcg
    41581 ccaacccgga accggtgcaa ccgatagtaa taacgaacag cgtcatcttt cctattggtg
    41641 cacggacgga aaaaatcatc ggccaacgcc ggcacaggag cgggcgtctg gaatggatcc
    41701 aatggggtgc catctggttc accgggctcg ggcttcctgt cgacgcgacc ggcgatttca
    41761 acgacggata cgtcatcctg atagaagtcg accaggcacg agatcagcat gtcaggcccg
    41821 tcttgcagct cgcgtgtggt aggcgtggcg attcgaaagg tttccgccac gggcgcgatg
    41881 tccgggaacg gacagagcgc aatgcgccgg ccgtcgtccg acacccgatg atccagtagc
    41941 caaaggcctg ggaggtgcca gtcgccaatg cgacagtgcc aggcgcccag cccggcggca
    42001 ttgtcgcgaa tccaggtgaa ttcggcctgt tctccattct ggaactcaca ggatttgact
    42061 cgtgccgcgc cacgcccgcc ggggctgtcc aggggcaggc acagccggac aggcctgcag
    42121 gggtatccgg caagccgcat tcgcccgtcg tcgtcgtggc caatgagaat cgccgatttg
    42181 tcacggaggc aggcaatgga atagccgtgg ttgccgtggc tgagctcggg gttggccagg
    42241 gtggcgcgca atcgcagctc gcgtgcgtcg agggttgccg cgctgtcctc ggtcaatgcc
    42301 ggctccccgg atgaagaagc ccagactgaa ggaagagccc gccagttttt cccgttttcc
    42361 caaagccaga agtttcgaag ttcgtatagg gattgtgccg gctcggtggc ctgccgtgcg
    42421 cggcaagcca aggcctggga gtccgcgcgc cacgagactg actcaggggc gtcggtcagc
    42481 aggccggtgt cttcaccgtc caaccacaga gcgtattggg gctcgtacag aggcttcaac
    42541 ggttcttcca gatcgcgcag cgaagcgggc acaaaatgcc tggccaccag tttgtgttga
    42601 tgatctggcg agaggtattc gtgggtgacc aggttctcca accgatgcga ccattgttgt
    42661 gggtccagcc aaaggtctcc cacccgcatc aggtcggaag cctgggttct ggccaggaag
    42721 tcaccgaaaa ccgggctggg gccggcccct tcacgaggtt tgtcgttggc gggcgactca
    42781 gcgaaatcct ccagcgccca gaacccgtcc cgattggtgc cttgatacag gcgccgcgtc
    42841 tcacggtcca ggatgatcag gccgtcggcg cctcgactgg ggcagatggc cgcgaagtag
    42901 cgcccatcga cggagaatcg gccgatgtct cccacacctt gaaggacgac accgtcaggg
    42961 aacagaatcg aatggaatgc gccataaccc atggccactt cgccccaatc caggaatcgc
    43021 aacggctggc cttccggggt ctgtctacgc tggaaatgcc ggtcggcgcg ggtcccgccc
    43081 ggtgggccgg caccagggag ctcaagggaa agccgctgtg ccaggcttac ttgcaggaaa
    43141 aacaccaggg ccaccaccgc gataccggga atcggccccc accagaacgg gaacagcaac
    43201 cacactcgca cacccaggcc cagcgccgcg cccgcgatcc agtccagcac ggcccgggcc
    43261 ggtttgcgcc cggccacgcg gaacgcggcg agcaccagcg cgaacaacgc ggcgatgaca
    43321 gcggctcccc acagggcggg aagcgcggcg acaatcgcga gtggaaaaac gatgaccgcc
    43381 agcgagccca gcacgcgcaa cagcagtgtg gacaacgacg ggccgtctac cttggatgga
    43441 tggggcatgg tttcatgggc ggtggccggt ggatgcttac gattggcgag cgacgaaaca
    43501 taactgtttg tgactgaaag ggtcaataca acagctatac aatccagtca cgagagcggg
    43561 gaggggccgc cttacaatgg cgctctcttt cattcctgca gccgcagtgt aatatggccg
    43621 cgccggcaca aaaagggagg cgcatgcgac agcctgagcg ggaagtgtcc tgcgctgagt
    43681 gtggggcgag gcatgcacgg tcgaacggcg cgggagacgg gggaaaaggc aacacatgaa
    43741 atcgttattc gcggcgctca tcatcttggt ggcgttgatc gccctggcga ttggtgtgcc
    43801 actcatcctt cagccggaca cgggcggatt cctgtctggc atcggggtgg tgctgggcct
    43861 ggttttcctg gctgccggca atttgctggt gctggccctg aatctggcgt ttcggcattt
    43921 ctatggggcg ccccgctggt tgcgtgtgct gatccgcatc cagttggtcc cggcggcggt
    43981 gtctctggtg atgctggccg tgcaacttta tgacagttgg caggagggtc gtgccgctga
    44041 tcagcatgtg gcgttgtatc tcgcgatcaa atccaatgac gtcggcaggt ttgatcaggc
    44101 acagcggcag tgcggcaaac aatgctccag tgactattcc ttgaacgcgc aattgctgga
    44161 tgctgcggac acccgcgccg gcaacgtggc agaggcgctc atccgtcagc acgcgaaggt
    44221 atccaccgaa ttgggccagg ccacggtgtc tctgcgcacg tgcgaggggg attacctggc
    44281 cgatctgaac gccctgggcg tcgcggtggc gcgtcacgac gcctccatgg tggatgagct
    44341 gtttcccgtc agtgataaag gggcccgccg gaaggcccta tgggtggcgg cgcgcctgaa
    44401 ccatctagat ctggtcaagg atcttgccgc gaaaggcgtg ccattaacga ttcgcggacc
    44461 gattctcaac gaaaacgata ccctgctcgt cgcggcggcc agcggtgcgg cgcttgaagt
    44521 cggaaaatgg ctgctcgata cgcaatacat gcccgtcaat gccattcttg gcggaccgga
    44581 tccctacccg ggtacgtcgc ctttgcaggc actgatgcgt ttccaatcct cagcgccgga
    44641 ttccccgcgc ataaagccgt ttcttcagat gctggtcgcg cacggcgcga aactggatgt
    44701 gcgtgatcgg gaagggcgca cgccactgca ggaagccatc cggatgaaag acaagcgcct
    44761 tgcgcaaatg ctgctgagtg tcggcgcggg aaaatcctcg ttgacggctg aagagggcaa
    44821 ggcactggaa gcgttgctcg cgcaacccgg cccgcttgtc tccccgccac attcgggagg
    44881 ggactgcgtg gcaccttagc cccgatatgg gcgctttggg tgtcaaacgc tcatgggctg
    44941 gcctgtacag ccagttcgtg ggactcgggt acgatcaaga cctgatcaca ggagtatgag
    45001 gactcttttg tcatgcgaac ttttgttttg gtgatcgcca gcttgctgct gtccttgctg
    45061 gcctccatcg tggccgcctt gggtgtcgca ctggtgtttt tgctggtttg ggaagcgttc
    45121 gacctatgtg ccgcgttcgg tggcccgatg gagggcagtt gcggctacgc cttctcgttc
    45181 ttcttcatgc cgatcattgt cctgtcgtgt ttgattttcg tcaccatact ggcgtatcga
    45241 aaaatccatc cctggctcgt gcgccggctg ggccctgtca acccggcacg ctgcgccgcc
    45301 cttgatcatc ataatccccc ggtttgattt tcgccgggtc cacctcgtgg cactgcacgt
    45361 cgcggagctc gtcagcgtcg ctgaagggtt gcacattcca ggagtggtcg atgcgcgcgc
    45421 cagccagcca gtgcgatcgg tccaccacac attccatcag tttgcactgg cctggctggg
    45481 ccagggcggg cgcgacgcag gcggaggccg gcccaagcca tgtcaggcca taggtggtgc
    45541 gggagcccca tagcggtctt tttgacgagt gattgaagac cagcgcctgt tcttccagca
    45601 cctcgggaac atgatcctcg accacgttct tgttgtgctg gacatagaat tccgccgggt
    45661 agcggaacag aaacagcagc gccaccacga ccaccagcag ccaggccgcg cagcttgtcg
    45721 accatgccag cagcgtgcgg ctggcgcgcc gcccaacgac cgcgctaatg ccgctggcga
    45781 gggccaacgt cgccagcaac agagtgaggt ggtccatgga ccaatgcggc gttctcaggt
    45841 agacgccgat gtgtggattg aaagcggtga tcgcggcggc gaaggcgaat acggccatca
    45901 acagcgagaa ggcgatgaag gaggacgagg gcttcttcgc acgcaattgc gttgggttgg
    45961 tggcgcttgt gtcagaaggc atgcgggcgc attcctttgt tacgtcgtga gccgcgcagg
    46021 cgcgctcgtt gatctgtttt cagttgagtc attttgtgag tattcgcaaa aacgaggcgg
    46081 tttaaatggc tgcggccaca ttgccagagc gtgctcttac aatcgcgatt gtctctacac
    46141 cagtatatcc ggatcaagga gggaaatatg ccgcgacctg tgtctcgcaa ccacctggcg
    46201 ccgctgttga tgctggcgat gttggccatg cccgtttccc cggcgatggc ccagcgcggc
    46261 agttgtgtct ccgaggaccc ctatgcggcc atcaaagcgg cctggcctga tccgacccgg
    46321 tcccgtctgg gcaatgtgtg catcaacgcg ctgcatgatg ggcgcgccag cgtgcggctc
    46381 cccagtgccg gggtgaacat cgacgatgcc gcggccagcg atggttcgac cgataaatgg
    46441 ggctatcttg acaaagacgg caagcttgtc attccgccca cattcgacat ggcgttgaac
    46501 ttccggcacg ggttggcggc ggtgtcgatg aatgggctat gggggtacgt ggacttgaat
    46561 gggaagtggg ccatcaagcc gcgcttcctg gaggccgacg cattcacctc gctgggcctg
    46621 gccatggtgc gtgaaggcgt ttcgtatgaa cttatcgatc gcagcggcac catggtggga
    46681 aagccatttc ccgcaggggt gaaggcctat ctggccgggg ggaagcccgg ttttgtctat
    46741 ctgtcgtatg ggcctgaatg gcgttcgtcc gccggggcga cgttctcccc gccggacggt
    46801 gtgttcgtag tgaagcccct gggggggcag gacttgttca tcgcgcgcag cgcttcccgg
    46861 cgctacggtg tcatggatgc gaacgggaag tggattgtcc tcccagactt ccaatcgctc
    46921 gacgcttccg tcattcccga cggcttggcc ctggcccggg gctaccgacg ctcagcgctg
    46981 atccgtgatg acggaacgta tgccacagac cggcacgtca agctcatccg tcggttgggg
    47041 aggcatttct ggctgacccg cgacaaccac ggcgtttcca ggttgttgga cgaacacggc
    47101 aaagtggcac atgacttcgg caaagcgccg ctgggctcgt ctctcgtgac acttggcccc
    47161 ttcgtgttgt ggtccaccga cacggactgg caggtcttcg tgcccgggcg cgccgcgccg
    47221 gtgtccattc cccgggcatt gcaacccaaa tggagtggtc cggagaatgg tgttctcctg
    47281 tggcgcaacc tcgacacgga tgccgtcgcc ggcatcctga cccgcgcggg gcgttggctg
    47341 gtcggcccgg gttggatcgc cgacatagct ttcaccattt cgctgggcgg gcagaccgca
    47401 ttcaccgatc gccggggcag tcttctcaat gtgctggatg ccgatggcaa tccgctgctg
    47461 acctctgacg ctgcgcgggg cctcgcgcat cgccaggtcc aaccgctcca cgatgcgcca
    47521 aactcttcga agactccggt cgccatcgcg attctcggcc cctgcgattg cgacccggaa
    47581 accggctcgg gtctgctgct gtcagacggg catgtcgtcc aggacaaggc gtggaaatcc
    47641 atacacagcc tcgaccctga cgactcgaaa acaagcaccc gagtttcagt agcacacacc
    47701 acgattcccc agcgattcgc cgtggacacg gctgctggca ccggcatgat ggatgtctcc
    47761 ggcaagatgc tgctcgcggc tgatcaaaag catataggcg ccttccagga tgggctggca
    47821 ctggtctctg ggcggaacgg tacacgtgtg atggaccgag ccggaaaact gtattccgtg
    47881 cccgactatt tcgccctgga atccgccggc gccgggttgc tgcgttttcg ggaaaccgcg
    47941 aagcccgacg cgttgtgggg gctgtatagc gtggctcagg ccgagatcgc ggtgaagcca
    48001 gcgttcaagt ccatcggagc cttcgaatca gggacggccg tggcgcagga ttcctcgggt
    48061 gccacgggtg tcatcgatac ccgtggccga tgggtggtgc ccgcccgtta cgcggcggtt
    48121 gaaccgttga ataggcaatt gtgggtcgtg cggggcggag cggcgcaaac cgacggcatg
    48181 aaaggcgtgg tggcgcggga tgaccaagtc gtggtgacgg catcgcgagg attgaaggtg
    48241 catcaaaacg aaaacggcct tatccgcgtc tcctttcaag atgcggagga tccgtcacga
    48301 cgcggcgcct ggctgctgga tcagtccggc aaacgcgtga tgggcgggtc gcacgccgac
    48361 attgaattca tgaaaccttg gtggctggtg ctgagaccgg actccggtgg ctacgtcggc
    48421 aaggacggaa attgggtcat tcccccggcc ccggggttgg gaacagcgtt ccacggatca
    48481 caaggcgccg ccgcgctcgc ggtcaggaac caggggaaga cgagtctcat catcgatgaa
    48541 aaaggaaggg tgcatgccac gcttcaaggc ggtgactggg cctggccgga tgggtccgac
    48601 tggttggttc ggcaggtcgg gcgcgacggg aaggagccac ctgaaaccct gtacgccgac
    48661 accggcggca agattcgtct gacggtgaaa ggggcggcca gcgagttcgt tggcgattac
    48721 gcggtgttgc gaagcgccga tggacgacac tacggttgga tcgacaagca gggccacgag
    48781 gggccggccc acgactatga ttggctgggc ttgccctcgg acggcctggc cttcgcgtcg
    48841 gagcatggcc tgtatggcta cctgaacgag cgcggccaat acgtgatcgc gccagccttc
    48901 aaggccgcca cgccattcgt cgatggccgc gccgtggtgt cgacccggct ggcgtcgatg
    48961 atcatcgaca ccaagggtac cccgcttgcc cgagtgacct atctatgcgg ggtgcgcgtg
    49021 ctgttcgatg ccaacaacca tgtggagtgg ccgaagacgc ttccgtcggg atgccgttag
    49081 ctcactaatc ggccgccgct ggcgccgtgt cgcccaactc ttcatcgcgc atcaacagcg
    49141 acaacaggat ggacacgcag gctgtggcgg tggccagggc gaataccttc cggaacgcgt
    49201 cgtcgaccga cgagaccaga tcggcgcgca ccaaggcgtc catggccgag gcggcttgat
    49261 caatcaggct tttgatgatt tcgggcccgg acaagccgcc ggcggtggcc ggcgccatgg
    49321 cttgcagggt ggccatgagc acggccatga gcacggcgat gccaatcgcc gctcccagtg
    49381 atcggcagaa cgcgatgacg gctgtggcaa tccccatgtg ccgtgattcg accgcgttct
    49441 gcaccgccac cgtggacgtg ggcagttgca ggccgatcgt gaagccgatc accccgacgc
    49501 acaccaggtt ggcgcccgtt gcgtgggcat cgacaaacac caaccccagc atggcgatgg
    49561 gcgtgcatac ggcgccgcaa agctggatac gcttgaaccg ccccgtgaat gccgacatcc
    49621 gaccacccac gtaagccccg accgggatgc ccagcgagaa aggcaacagc agcagcgccg
    49681 cctggtcggc ttgggcgccc agcagcattt gtgcccgcag cggaatcagc accgagagcg
    49741 agacgatgag gatgaacgcg atgaacatca gcgtgcagga cagtgacacg gtgggattgc
    49801 ggaataggcc aagcggcacg atgggttccg tggcccgcgt ttcctgccac agaaagagcg
    49861 cgatggccac cagggtgcca ccgaacagcc agacgttcaa ttcatccatc caagggctgc
    49921 cttgcccgac ccgggtgatg cctatcagga gcggcgaaag tgccacggtc atgaggatgg
    49981 cgcccgggta gtcgacgggt ctctttatgc ggggcgtatg caaccgggcc agggcgcgcc
    50041 gcgagatgaa catggccgcg gcacccaatg gcaggttgat ccagaacacc cagcgccaag
    50101 acaggtagga cgtcaggaat ccacccagta gtgggcccga cacgctcgcg acggcgaacg
    50161 ccgcactgat gtagccctgg taacgacctc gctcccgcgg tgccaccacg tccgcgatga
    50221 tggcctgtgc cacggaaatc aggcctcctc cgcccaaccc ctgcagaatg cgcgccccga
    50281 ccagaaaggg catggatggc gccatggcgc tgcctatgga ggaaagcaga aagatggtga
    50341 tggccgacgt cagcatgacc cgtcggccgt acaagtcacc gagcttgccg taaataggtg
    50401 tcgccacggc cacggtgatg aggtagcccg ataccaccca ggccagcagg tccacgccgc
    50461 ccaggtcggc cgacatcatg ggcagggcga ccgagacaat ggtctgatcc agcgcgccca
    50521 gcaggatggc cagcatcaaa ccgatgatga tggagcgaac ttccgcatgg gaaaaagtct
    50581 ggggcttggt cggggcttga ttcatgttct gtgatgccgt ggccgaggtg atggtgcaga
    50641 gcctggccct gttttcaggg ttaaccatta ttgtattcaa gaatcgaatg cgacatttca
    50701 acgtcgtttg gaaaatcgct ctgtctgggt gaaaaagcgc tggcaggcac cgccctggaa
    50761 gcgtggtggt cggggcgcaa gccgtggacg agactacgaa agtcgccgac gaggctacaa
    50821 tccccgccac catgcatcat gtcaacgacc agattgtcct cgacgaccgc gatgtcacat
    50881 tcaccatgat ccgggctcaa ggagccggcg ggcagaacgt gaacaaagtg gccacggccg
    50941 tccatttgcg cttcgacatt tccgcgtcat ccctgccgga cgactgcaag gcggtactgc
    51001 tggcgctacg tgacaggcgg gtatcagggg atggcgtcat cgtcatcaag gcgcagtcct
    51061 tccgtaccca ggaaaagaac cgcgccgacg ccattgaacg cttgctggct ctgatacgtg
    51121 gcgcgttgaa gcagccggcg ccgcggagac ccacgcgccc gacccgggcg tcgcagcgcc
    51181 gacgcgcccg gaacaaggca cggcagggcg aggtcaagcg cttgcgtgcc aaggttgccg
    51241 aagcaccgga ataagtgcgt ttattgcttc ttgttcatgg gctggcgtcc atgaaacgtt
    51301 tcaggtgagt gatgtcatgc ctgcgcacgc tcacgctccg cgtctgagtc gggctccttc
    51361 agcatgtcac ggatatcagc ggccagcagt tcgttcatgc gttcgctgct ttcccgcgtc
    51421 gcgatggtga agcgttcctc gtcaccccag acctcggcca gcgcttcgaa catgttgacg
    51481 tcgtgtttgc gaaacagctt ggcggcgcgc acagcctgcg tgcctttgaa accgagaacc
    51541 ttcaatgcct cggcacccag tgtcaggctg gcctcgaagg tttcacgctc tatgatgtgc
    51601 gcgcctttgt cgagcaggcg gtacgcatgg cgccggtcga atgcgcgcgc cagcaccttg
    51661 aggttgggga aattcgcgcg cgcggtttcg accagttcct ccgcctggtc ccgatcatcg
    51721 atggcgacca ccagcaaccg cgcatcggcg gcaccggccg cctgcaacag ctccaggcgt
    51781 gtggcatcac cataatagac gcgccgtccg aaacggcgga ccagctccac ctgctccgca
    51841 ctggcttcga gcgtggaggt cttgaagccg ttgacgatga gcagccggcc gacgatctgg
    51901 ccgaaacggc cgaagccggc gatgatggcg tcgggacggc tgtcgaacgc ttcattctcc
    51961 ggcctcgccc cggtggatgg agagaacccc tgggtgcacc atttttggta ccccatgatc
    52021 aacagtggtg tcagcgccat ggaaatcgcc acggtggctg tcgccagcgc cgcgatctgg
    52081 ccggggatga tgcccagcat cgccaccagg cccagcagga cgaacgcgaa ttcgccaccc
    52141 tgggccagcg cggcacccat cattgccgca gagcgttgtt ccattccgaa tagccgggcc
    52201 atgccgtaga tcacgacggc cttgatggcc atcaggctga ccacgcaaag cagcagtgtg
    52261 agaggttgtg aaaccagcag ccccaggttg atgcccgctc cgactgaaat aaagaataag
    52321 cccaggagca gggcgcggaa cgggtcgatg tcgctttcga gctcgtgccg gaattcgctg
    52381 tcggccagca ccaccccggc caggaacgca cccagggcgg ccgacaggcc aaccgtgcgc
    52441 atcagcgcgt cgacggcgac aacaagcagc agggccgagg cggtgaagat ctcgcgcgaa
    52501 cgggtgttgg cgatgaaacg gaaggccggg cgggtgagat aacgccccgc gctgatgatg
    52561 agcaggacgg ccgccaacac cgccaggcca tgcacccact gtggttgccc cgcgagcaag
    52621 ccggtgctgg cggctgttgg cgcgatatgc tccgcggcca gcaggggcag ggcggcgaac
    52681 atggggatgg tcgacacatc ttggaaaagc aggatgccga acgctgcgct gccggccgcg
    52741 gtgtgccgca ggcccttttc ctcaaaggtt tgcagcacga tggcggtgga ggacagtgac
    52801 aggatcaagc ccagggcgac cgccgcctgc catgattggc ccagcacgag gcatgcagcc
    52861 gccagaacca gcccggtgac agccatctgc gcgccaccca ggccgaaaaa cacgccacgt
    52921 agacgccaca gcattgaagg acgcacctcc atgccgatca ggaacagcat gatgaccacg
    52981 ccgaattcag cgacgtgcat gacgctcgac gtgttgcccg ccacgtccag cagtggaccg
    53041 atgatgaccc ctgcgaccag ataacccagc acggacccca gccccagccg ttgggcgatc
    53101 ggaaccgcga ccaccccggc ggcgaggtag accaatgcgg ttagcaagag atcggggcct
    53161 tgaacgatgg ggggctcctg aagggtgggt tggaaggctg tgccgccgag tgcggcaagc
    53221 cgtacccggc taccgcgtca gttctggatt ttccacgata tcgggctctg tcagcaactg
    53281 cccgatcacc gctcccaggc gcaggtcgtc catctggcca aagtggcgca gccgtatgcc
    53341 gccgttgcgg tcgatgacca ccaggctggg cgtgccttcg aaccggtagg cgcgcatcgt
    53401 catggggatt gggtcgccat cctgtgcggc ttggtcgatg ccgatgggaa aacgcaaccg
    53461 atactcgtgc gcgaacgcgc gcagcgcaac gggagtcatg gcgtcatgat gttcgaacac
    53521 ggtgtgcagg ccgatgacct tcaccttgtc ttgtggaaat gccgcgtgca cggcgcgagc
    53581 ctggggaagt ccgtgggaca cgcacccggg acagagcatc tggaacgcat gcagaaccac
    53641 cacgctgccg cgcaacgact ccagggtgat gggggtgggg gtgttcaacc actcgctgac
    53701 cacccaggcc ggtgcgagtt cgcagggtat cttgtccatg actccgactg tcattgattt
    53761 gttgaaaaca ggcgtattgg aagcgcacag caatcatcat agacctgtcg gttggagcgt
    53821 gcggcggatg cgttacggtg cgcttggcga tgccgtatcc actgtggctt ccacggctgc
    53881 gggtgggatc gggcaatgga tgtctgtcga cttaagcgct atgcctgtcg ccgcaggcga
    53941 agccgggtga tctcggagcg ggcgcccaga cgcatgggag gcccccagta gccggtgccg
    54001 cggctgacgt agatcaacag tttgtccagg cgatgtagcc ccgccacata aggctgttgc
    54061 aacgacacga aatacggcca gggccagaac tgcccgccat gcgtgtggcc cgaaagctgt
    54121 agatcgaagc ccgccttcgc ggcatcggcg gcggtgcgcg gctggtgcgc gagaagaatg
    54181 cgggcagcgg cttcacgtgg cgccgcgcgc agcgccgcga cggggttgca ggcttgtctc
    54241 gagtcgaacc gctcggcggt gaaatccgtc acaccggcca cgaccaacag ggcgccacca
    54301 cggtggatca ccctgtgctg gttcatcagg acgtccattc cgaggcggcg gtattccgct
    54361 acccaggcgc ccgcgccgct gtaatactcg tggttgccgg taaccgcgta gacgccctcc
    54421 ttggcctgaa ggcccgacaa gggggccacg tcagacgcga gcaacgccac actgccatcc
    54481 accagatcgc ctgtgagcgt gatgatatcc ggcttcaagc ggttcaccgc gtccaccacg
    54541 cgacgcacgt aggggcgttt gatggtggat ccgacatgca gatcggatat ttgcgcgatg
    54601 gtgaaatcat gcagactgtc aggcagatcg gggatgggta cgtccacgtc cacgagccgc
    54661 ggcatgcggc gggcgttgaa tagccccagc agcaccgcga gcatggtgag aatcgggacg
    54721 gccagcgcgg tgatagccgg cgcgtggttc gtggcctctt tcagagacgg tgtgatcgcg
    54781 ctgccaacca tcagcaagaa caacactgcg tcgcgcagga gcgtcagcac gaacagccac
    54841 gaaaaacatc ccaacgccag gaagccgctc caagagagca catcgcccga taatcggccc
    54901 gcctggcgcc gcgccaggaa tcccgccagt atcagtgcgt acaaggcggc gatcaccacg
    54961 ggaacgaccc attggacccg ggttcccaac gcgggttcga tcaaccgcac cagcacgtat
    55021 aggtgggcga gcagcaacag cagcggaatg cgtcgaagga gcgtcggtcg gctcatgtca
    55081 ggtgccttgc atcgaaattc aggaatcgcc gcgcgcggcc ctgaatcgcg tgcccgcgta
    55141 agtggccagc aacaccagcc cggtggagat cagaatcatg acgattccca gggctgaaag
    55201 cttgccccac aacccgtcat cggcgaaatt caggatctgt accgccagga cctcgctccc
    55261 ggggcgcgac aacacgacgg aaacggtgag ttcccgcacg aacatggtgg ccatcagtat
    55321 ccacgccgac acgatgccgg gaaccagcaa cggcacgatg atgcggcgca tggtggtcag
    55381 ggcgctggca ccgcacacct gcgaggactc ttccaggtgc gcatgaacct gcacgaaagc
    55441 gctggccagc ggccggacgc catacggcag gtaggtggcg atgtaggcca ccagcagggc
    55501 ggtgagcgat gcatagagtg gcgtctgcac gaagaaccac atgaagccta tgccgatgac
    55561 gatgcctgga aaagaaaacg atagaaagct cagcgattcc agcagaccgg cggcgcgcgt
    55621 gcgtatcttg acgatgacgt aggcgatgaa gatggacaac agcacgccca gcgtggcgcc
    55681 gacaatagcc aggaaaacgc tatggcgcaa ggccagcatc gaggcgggat cctgcaggac
    55741 ttcggcccaa tgtttccagc tcatcatgga gaaggcatgg gcgcttggga ccatggagta
    55801 gggcacgagc gaggtgtaga agagtacggc cacgggtagt acggccatca ggaacgacag
    55861 cacgccgatg gcgatgaaca aaggcgttct ggctcgcttg agatcgatca ccgacggtcg
    55921 atagcctcgg cccgagatcg tgacgaactt cccaccttcg gcggtcaggg cgcggtacag
    55981 cacgatgagg ccgatggaag cagccagcac gctcatgccc agggcggccg ccttgccata
    56041 gtcgggtgcg aaaccggtgg cgatcatctc gtatagatag gtggccagaa cgtcgacgcg
    56101 ccccggcatc cccagaaccc gtggcaccgc atacgacgcc aggctgcgca cgatgccgag
    56161 cacgaaagcc gacagaatgg ccggccgcag cacgggcagg gtgacgcgaa acaaggtgcg
    56221 ccacacaccg gctccgctca cccgcgacga ctcctccagc gccacatcga acgatgccat
    56281 cgcgggcgcg atgatcaagt aggcgatggg catgttcagc agcccctcga ccaggatcat
    56341 gccttccagg gaatagatgt tgaacggtgc gccgtgccgg cccagcgcgt ctttgagaaa
    56401 caagttcaac aacccgttgg agggattgag cagcagcgcc caactgaccg cgaacagcag
    56461 atgcgggatc atcatgggca cgatggacag caccgtgaac atcggcttgc acggaatgtt
    56521 ggtgcgggtg ttcaggtagg ccaggaccaa cgccagcaag gtcgcgagca acgtggaacc
    56581 cagcacgaag atgattgtgt tgccgatcac cttgagcaga tacggatcgg tgtaggccga
    56641 cacatacttg gcaacggtga agtgtccgaa agccgtcaag ccctgcgaaa aactgcccag
    56701 caacagcatc aggacggggc acaacgtcag gagcccgacg atgacgatga gggaccaggt
    56761 gagcccggtg cgcgattgat tcatggtgcg cccgcctcag gccgagacga gcaggcaatg
    56821 cgccggggtc agcgcgaaac gaaccatgtc gccttgcgcc accctgacgc caggatcgac
    56881 gcgagccagc agcaactggt cgcccacgcg gacttccgcc tcgtagactt cgcccacgaa
    56941 gtccaatgtt tcaatgcatc cgctgatcag gttgtgaacc acgtcggtac catccccgcc
    57001 gcccgtgctg gcggtgacgg atccttggtt tgcaccatgg gtttcatccg cattggttaa
    57061 caacaggaat tccggccgga tgcacaaggt ggcctgggca cccaccggca ggtcgcgctt
    57121 ctggcagcgc atcacgccaa gtggcgagtc gaccacagtg gcgtccgctc tctgctcccg
    57181 caccgtggcg gacaccagat tggcgcgacc gatgaagtcc gcgacaaagc ggtggtcggc
    57241 gtcgaaatag attttctgag ggctgccgcg ttcgatgatg tggccgccgc gcatgacggc
    57301 gatggaatcc gacagcgcca gcgcttcgac ccggtcgtgc gtgacgtaca cggccgtgat
    57361 attcagtccg gccaggaaat ggcgcagctc ttttcgggtt tcttcccgca gcttggcgtc
    57421 gaggttgctg agcggttcgt cgaaaagaat gaccttgggc tcggcgacga gggcgcgtgc
    57481 caacgcgacg cgctgctgct gcccgccgga tagccgtgtg gcggacctgt tttcgacacc
    57541 ttccagttgc acgaagctca atgccttggc gaccttctcg cgtatggtgg cgcgccgctc
    57601 gccgcgcacc tgcaacggat acgccacgtt gtcgaacaca gtcatgtgcg gccaaatcgc
    57661 gtaggtctgg aacaccatgc ccaggccacg tttttccggc ggcacggtga tattcttgct
    57721 ccgtgaccac atcacctcgc cgccgatgct gatctctccg tcgtccggcg tttccagccc
    57781 cacaatgcat cgcagcaaag tggtcttgcc gcagccgctg gggcccagca gcgtgaagat
    57841 ctgattggcg gggatggtga gatcgatccc atcgagcgcc cggacggatt taccttcgga
    57901 tatgtagttc ttgaccaggc cttcgatgcg gatttccatg cgttgccgtg cggcgtcgcg
    57961 ccgcccttct tgaggtggat gggacccgcg cgggtggagg cgggtgccga aggactatcg
    58021 ggaaccgaag atcttgccga actcgcgacc ccatttctcg atttctgtgt ctgacaggtc
    58081 acgcacaggc aagaccttga ccttgtctat gccgtcgatg gctggaaaca cccctggcgc
    58141 caacacgtat tcgcccacgt ttttcgccag cagactcatg gcttctttcg acaaccagta
    58201 atccatgaaa gcacgggcag catccggacg tggcgccttg ctggtgacag cgatggcgcg
    58261 tggcgtaccc agcagcggtt ggccgccgcg cgggccccaa tccagcggtg ccggcgcttt
    58321 ggtgacgatg tacttcggca tggagatgcc gatgagcttc tcgccgcttt cgatcggtgc
    58381 cggggtcggg ccgaacgacg ccacaaacat gggttggttc gccgccagcc ccttcacata
    58441 ggccatccag tcggattcgg acttgaagac gaattccttc agcccaatga gccaggaaat
    58501 cgtcgacgaa tgcgtggccg ggttggccat cacgatctgg cctttccatt tcgcgtcggc
    58561 caggtcttca tagcgcttgg gggcatccgc ccgcttgacg tgatcagtgt tgtacaggta
    58621 tgagacgtac tcgatgccga acaaggtcat gcgcgggtct ttcacggccc aatcgggata
    58681 gcccttggcc gacggcgact gataggccgc gagcacgcct ttttccttca gcaattcgag
    58741 catggggcgc ggcgcctgga cgacgtcggc cagcagcttg ccggcgttga attccgtcag
    58801 taccgtgggc aggaatttgg agctggaaat acgggtgtac tggcccttga tgcccgtggc
    58861 ggcatcgaaa gccttcatga cggattcgac cgacgtgatg ttggcgtaga acactgcctt
    58921 gccttcggcc ttggcttttg ccgaaacggc ttccgccaga gaaacggatg gcagggcgcc
    58981 cgccagtccg gcgacgccgg ccgcgccagc cagtttcagg aattcccgac gatcaaatgc
    59041 gttcattgtg agtctccggt ttgtattcgt gctttcgggt catggcttgg ttcccgtgtg
    59101 cgatgctcgc gccgcgtcgt tctgttgtcg cgtgggacca ggccagccgc agtcgtgccg
    59161 caaggcagcg atgccgcaat gatagcagtc cacatcgtcg ttggtaacgg ctggtcacac
    59221 aattgcgctt gtggccggac atgtgtattt cgcacactag gcaggtgcgc ggttcatcca
    59281 tcgcccccga atcctggccg cgcaggcgtt cgacggcaca gcccgtggcg ccgcgaggct
    59341 tcgcgaaccg gcggagcggg gaaggcagct tctccttcga gggcattcaa tggaaaggag
    59401 cctttcaatg cggacattat ttcaatccac caggcacgcc ctgctcgcgg ccggcttggc
    59461 cgccggactg ttcgccgcca gcggcgcggc gggtg
//
LOCUS       DASTNK010000143        10943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1042996, whole genome shotgun sequence.
ACCESSION   DASTNK010000143 DASTNK010000000
VERSION     DASTNK010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10943
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042996"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VFR20_10780"
     CDS             <1..918
                     /locus_tag="VFR20_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEU0231048.1"
                     /translation="DDVFTYESENQVFHAPRTLVQLAELRAQYPDARILAGSTDVGLW
                     VTKQMRDLPHLIYVGQVAELREIREEDGALYIGAGALLDDAFGDLLPHFGELDELVRR
                     FASLPIRNAGTLGGNVANGSPIGDSMPALIALGTRIVLRRNNTVRDMPLEDFYLAYQK
                     TALQPGEFVQGMRVPLPAAGQHFRTYKLSKRFDQDISAVCAAFAIVLDGETIREARIA
                     FGGVAATPQRASQAEQVLVGQPWNETTALRAMEALQQDYTPLSDMRATNDYRLAAAAN
                     LLYRFYLETRPLDPLSAQQTSVFAQPVDA"
     gene            928..3312
                     /gene="xdhB"
                     /locus_tag="VFR20_10785"
     CDS             928..3312
                     /gene="xdhB"
                     /locus_tag="VFR20_10785"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HEU0231049.1"
                     /translation="MNIRSDAFMAVAGQSQAKSAAGAPLPHESAALHVSGEATYTDDI
                     PEIQGTLHCALGLSQKAHARVTGMDLDAVRRAPGVVAVLTAADIPGENNCGPVVHDDP
                     LLADGLVQYIGQPVYAVVAETQDAARRAARLGVLQYEELPAILSPQQAKAENASVVPP
                     MALRRGDPDAAIAAAPHKLSGTFECNGQEQFYLEGQVSYAVPKEDHTVDVMCSTQHPS
                     EMQAVVAHALGWHSHQVRVECRRMGGGFGGKESQSALFACVSAIGAVRLRRPVKLRLD
                     RDDDFMITGKRHGFYYTYDIGFDDDGRILGAKVDMTLRSGFSADLSEAVATRAVCHFD
                     NAYFVHDVDIKAMCGKTNSQSNTAFRGFGGPQGALMMEVMLDMIARKLGRDALDVRRL
                     NFYGIGERDTTPYGEVVEDNVLNPLVDELEASSDYRARREAVREFNRTSPVLKKGLAL
                     TPVKFGISFNVPAFNQAGALVHVYRDGSALVNHGGTEMGQGLNTKVAQMAASALGLSL
                     DHMRVTATDTSKIANTSATAASTGSDLNGKAAEDAANRIRRRLAQFAAKKWGGEADDV
                     LFANNSVQVNGQTLPFREVVEAAYWDQVQLWSEGFYATPGIVWDKKTMTGRPFYYYAY
                     GAAVSEVIVDTLTGEHKLLRADALHDVGQSINPAIDIGQVEGGFIQGMGWLTTEELCW
                     NKDGRLTTHAPSTYKIPAVSDCPADFRVKLFANRNVRDSVFRSKAVGEPPLLLPFSVF
                     LAIRDAVASVADYAFEPELNAPATPEAVLNAVVGVRRQAAVARAAAGSKDAVHA"
     gene            3305..4378
                     /gene="xdhC"
                     /locus_tag="VFR20_10790"
     CDS             3305..4378
                     /gene="xdhC"
                     /locus_tag="VFR20_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008350887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HEU0231050.1"
                     /translation="MHEWVEQALRLLARGEPAVLVTVAKVQGSTPREPGARMLVAATR
                     QWQTIGGGHLEWRALSIARLMLQNKSRQRQIERMALGPSLGQCCGGVATLAFELLGQA
                     DADWLNDLSTRLKSGQASVRTVLFPADVSVPAVLPGVTLAAPEAAGVVEQPGESGTVG
                     QPGESCALWQAGGQQGLAEVIAPPRFNIVLFGAGHVGQALVRILGTLPCTVTWVDERD
                     TQFPDDIPANVTVEDTDTPEVVIEQAPPDSYFIVMTHNHALDQHLCEHIFKRTDYAWF
                     GLIGSRTKRHKFAHRLLARGVSAERFEKMVCPIGMAGITGKQPAVIAVAVVAQLLAEH
                     EARANTLQPDISATIGTPVTPAR"
     gene            4421..5758
                     /gene="guaD"
                     /locus_tag="VFR20_10795"
     CDS             4421..5758
                     /gene="guaD"
                     /locus_tag="VFR20_10795"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HEU0231051.1"
                     /translation="MTSTHASRAPVAHGPRTAYRAQLLYFKQEGPQTYSTVHLTDGLL
                     EVENGCVARVGDHAALAPGLSPDVQVVDLRGKLIMPGFIDTHIHYPQTDMIASPAPGL
                     LPWLEKYTFPTERRFENPEYAADVARFFLDELERCGTTTALVYCTVHPGSAEAFFAES
                     NARNLRMVAGKVMMDRNCPEFLRDTAESGGRDTEAIIQRWHGKGRQMAALTPRFAPTS
                     TPEQLGVCGELAQRYEDVFIQTHVAENTDEVKWAQELFPGHRSYLDIYDHYGLLRRRA
                     VYGHCVWLDDTDRRRMAETGAVAAHCPTSNQFLGSGLFDFAAAEATHMPTTLATDVGG
                     GSSFSMLQTMNEAHKVARLSGYHLSAERMFYLATEGAAQALDLGGRIGTLAEGAEADF
                     VVLDPQATPLLARRTASADSLEELLFAFAMLGDDRAVQATYAAGHCLRTRAAA"
     gene            complement(6077..7018)
                     /locus_tag="VFR20_10800"
     CDS             complement(6077..7018)
                     /locus_tag="VFR20_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0231052.1"
                     /translation="MASRHSNELLDTYLLKVLCMLVAERSVSRVALKLNQSQPAVSVA
                     LRHLRDILGDPILVREKGGMVPTERALSLVSHARSALAEIERMTAEPEGFDAQSSKQV
                     FRIGSPDYLAPVFMGRVSEQLRAMAPQASLTVHALGPEFDFERSLAEGELDVVIGNWP
                     EPPDRMHLSMLLEDDIVCLVSAGHTLAEKGMTVDDYRRAAHVVPMPYSISQRGVIDSA
                     LAAERITRDERVIVQSFNLAPYLLPGTDLVFTTSKHFALFYSRILPLRIIPSPIAFPP
                     MRFYQLWHERNHSSEAHRWLRRLLSECGVRVDAESAG"
     gene            complement(7180..8220)
                     /gene="alc"
                     /locus_tag="VFR20_10805"
     CDS             complement(7180..8220)
                     /gene="alc"
                     /locus_tag="VFR20_10805"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HEU0231053.1"
                     /translation="MAKPNLPTGTVINAAFVEPPAFATRCANLASADFGSRVVACSDE
                     FFAAAERALQPSEPVFIVGKFDDHGKWMDGWETRRRRNGGHDWAVVRLGLPGAIRGLD
                     IDTSHFTGNFPPAASVQACRCEGDPDDKTVWTQIVAAQSLAGDSHHFVEIDNDARVWT
                     HVRLNIFPDGGVARFRVYGQPACDWEKQDPKALHEVSALVSGGRIVAYNDAHFGVPFR
                     LIMPGRGVNMGDGWETRRRREPGSDWCLIELGHAAIVRKIEIDTAHFKGNYPDRVSIQ
                     AASVAESTDQSLVTQAMFWPELLSEQKTQMDHQHFFEGESIHDLGPVTHVRVNMFPDG
                     GISRVHIWGELA"
     gene            complement(8402..9334)
                     /gene="puuE"
                     /locus_tag="VFR20_10810"
     CDS             complement(8402..9334)
                     /gene="puuE"
                     /locus_tag="VFR20_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HEU0231054.1"
                     /translation="MAQKDYPRDMIGYGRTPPQANWPGKARVAVQFVLNYEEGGENCV
                     LNGDPGSEQFLSEIIGAAAYPDRHLSMESIYEYGSRAGVWRILREFERRGLPLTIFAV
                     GMALERNPEVAQAFVELGHEIACHGYRWIHYQNVQESIEREHMQRCIEVVTKLIGHHP
                     EGWYTGRDSPNTLRLVAEEGGFLYDADYYGDDLPFWTSVDLGNGRTRPQLMVPYTLDT
                     NDMRFAAPQGFNSGEQFFTYLKDAFDVLYAEGETTPKMLSIGLHCRLTGRPARFAALR
                     RFLDYIQKHDRVWIPTRAAIAHHWAEHHPWQGGN"
     gene            complement(9348..10106)
                     /locus_tag="VFR20_10815"
     CDS             complement(9348..10106)
                     /locus_tag="VFR20_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0231055.1"
                     /translation="MLNLNPHRDVSADLEAPRASSWRNVRGGVKSEVDRVYDDIFDAV
                     MDRRLLPGAKLTEATLCSVFACPRSTVRTVLSQLAHDKIVVIEPNRGAFVWQPSAKET
                     RDVFEMRQAMECMTIDMLLALPDLAERLAPLHDMVERERKAFECGDRVSWIRLSNAFH
                     VEMAHLLGNAVLTEMLHSLCARTSLIIAHYDTPGESTCSYVEHLDILEQLAGNDAQGA
                     KAAMRHHLEDCEHRMNQPEGAQASDPWLAFSVKR"
     gene            10216..10569
                     /gene="uraH"
                     /locus_tag="VFR20_10820"
     CDS             10216..10569
                     /gene="uraH"
                     /locus_tag="VFR20_10820"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006495593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEU0231056.1"
                     /translation="MGKISTHVLDTVRGKPAKGVRIDLYRVEADHRELLRQTATNHDG
                     RCDEPLLQGDALRKGVYELVFHAGDYFAELGVPADEPRFVDQITLRFGVAHPDQNYHV
                     PLLVTPWTWSTYRGS"
     gene            10609..>10943
                     /locus_tag="VFR20_10825"
     CDS             10609..>10943
                     /locus_tag="VFR20_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEU0231057.1"
                     /translation="MEAFLFEYGNLLLRWLHVIAAIAWIGESIYFVMLDNSLHPPHGA
                     ESKKRGVFGEMWAVHGGGFYHNQKYLTNPAELPDDLHWSFWKAYTTWLSGFGLFFIMY
                     LLKPEFYLV"
BASE COUNT         1963 a         3551 c         3524 g         1905 t
ORIGIN      
        1 gacgacgtgt tcacctacga atccgagaac caggttttcc acgcgccgcg cacgttggtg
       61 caactggccg aactgcgcgc gcagtatcca gatgcgcgca tcctggcggg cagcaccgat
      121 gtggggctgt gggtgaccaa gcagatgcgt gatctgccgc acctcattta tgtgggccag
      181 gtcgccgaac tgcgcgagat ccgcgaggaa gacggcgcgc tgtacatagg tgccggtgcc
      241 ttgctcgatg acgcgtttgg cgacctgctg ccgcatttcg gcgagctcga cgaactggtt
      301 cgccggttcg cgtccctgcc catccgcaac gcgggaacgc tgggtggcaa cgtcgccaac
      361 ggctcgccca tcggtgattc catgcccgcg ctcattgcgc tgggcacgcg tattgtgctg
      421 cggcgcaaca acacggttcg cgacatgccg ctcgaggact tttacctggc ctatcagaaa
      481 acggcgctcc agcccggtga gttcgtccag ggcatgcggg tgccgctgcc ggcggcgggc
      541 cagcacttcc gcacatataa gttgtccaaa cgttttgacc aggatatttc ggcggtgtgc
      601 gcggcgttcg ccatcgtgct cgacggcgag accatccgcg aggcgcgcat cgccttcggc
      661 ggcgtggcgg ccacacccca gcgagccagc caggccgaac aagtgttggt cggccaaccc
      721 tggaacgaga ccaccgccct gcgggcgatg gaagcgctgc aacaggatta caccccgttg
      781 tccgatatgc gcgccaccaa cgactaccgg cttgccgcgg ccgccaacct gctctatcgt
      841 ttttatttgg agacacgccc gctcgacccg ttgtccgcgc agcaaaccag cgtgttcgcc
      901 caacccgtcg acgcctagga ggccgccatg aacattcgat ccgatgcctt tatggccgtg
      961 gccgggcaat cacaggcaaa aagcgcggcg ggcgccccgc ttccccacga gtcggccgcg
     1021 ctgcacgtga gcggcgaagc cacctacacg gatgacatcc ccgagatcca gggcacgctg
     1081 cattgcgcgc tcggtctgtc gcagaaggcc cacgcgcgcg tcaccggcat ggatctcgac
     1141 gccgtgcgcc gcgccccggg cgtggtcgcc gtcctgaccg ccgccgatat tccgggtgag
     1201 aacaattgcg gcccggtggt gcacgacgac ccgttgctgg ccgatggcct ggttcagtac
     1261 atcggccagc cggtctacgc cgtcgtcgcg gaaacccagg acgccgcgcg tcgggcggcg
     1321 cgtctgggcg tattgcaata tgaagagctg ccggcgattc tgtcgccgca gcaggccaag
     1381 gcggaaaacg cctcggtggt gccgcccatg gcgctgcgcc ggggcgatcc ggacgcggcc
     1441 atcgcggccg cgccgcacaa gctctccggc acgttcgagt gcaatggcca ggagcagttc
     1501 tatctggaag gccaggtgtc ctacgccgtg ccgaaggaag atcacacggt tgacgtcatg
     1561 tgctccacgc aacatcccag cgaaatgcag gcggtggtcg cgcacgcgct gggctggcac
     1621 tcccaccagg tgcgcgtcga atgccggcgc atgggcgggg gcttcggcgg caaggaatcc
     1681 cagtcggcgt tgttcgcctg cgtgtcggcc atcggcgcgg tgcggttgcg ccgaccggtc
     1741 aagctgcgcc tcgatcgtga cgacgacttc atgatcaccg gcaagcgcca cggcttctat
     1801 tacacctacg atatcggctt cgacgacgac gggcgcatcc tgggcgccaa ggtcgacatg
     1861 acgctgcggt ccggcttctc ggccgacttg tccgaggcgg tcgccacgcg cgctgtctgc
     1921 cacttcgaca acgcctattt cgtgcacgac gtcgacatca aggcgatgtg cggcaagacc
     1981 aacagccagt cgaacacggc attccgtggc ttcggcggcc cgcagggcgc gctcatgatg
     2041 gaagtcatgc tcgacatgat cgcccgcaag ctggggcgcg atgcgctgga cgtccggcgc
     2101 ctgaatttct atggcatcgg cgagcgcgac accacgcctt atggcgaggt cgtggaagac
     2161 aatgtcctga atccgctggt tgacgaactc gaggccagca gcgactaccg cgcgcgccgc
     2221 gaagcggtgc gtgaattcaa ccgcacaagc ccggtgctga agaaggggct ggcgctcaca
     2281 ccggtgaagt tcggcatttc cttcaacgtg ccggcgttca accaggccgg cgcgctggtg
     2341 catgtctacc gcgatggctc cgcgctggtc aatcatggcg gcacggaaat gggccagggg
     2401 ctcaacacca aggtggcgca gatggcggcc agcgcactgg gcctcagcct ggatcacatg
     2461 cgcgtcaccg cgaccgacac cagcaagatc gccaacacgt ccgccacggc cgcctccacg
     2521 ggatccgact tgaacggcaa ggcggccgag gatgccgcca accgcattcg ccgccgcctg
     2581 gcgcagttcg ccgccaagaa gtggggcggc gaggcggacg acgtgctgtt cgccaacaac
     2641 agcgttcagg tgaatggtca gacattgccg ttccgcgagg tggtcgaggc cgcttactgg
     2701 gaccaggtgc agttgtggtc ggaagggttc tacgccacgc ccggcatcgt ctgggacaag
     2761 aaaaccatga ccggccggcc tttctattac tacgcctacg gcgcggccgt gtccgaggtc
     2821 atcgtcgaca ccctgaccgg ggaacacaag ctgctgcgcg ccgatgcgct gcatgacgtg
     2881 ggccagtcca tcaacccggc catcgacatc ggccaagtcg agggcggctt cattcaaggc
     2941 atgggctggc tgaccaccga ggaactgtgc tggaacaagg atgggcgcct caccacgcat
     3001 gcgccgtcca cctacaagat tcccgccgtc agcgattgcc cggccgactt ccgcgtgaag
     3061 ctgtttgcca atcgcaacgt ccgcgactcg gtcttccgct ccaaggcggt gggcgaaccg
     3121 ccgctgctgt tgccgttcag cgtgttcctt gccatccgcg acgcggtggc cagcgtggcc
     3181 gattacgcgt tcgaacccga gctcaacgcg cccgccacgc ccgaagccgt gctgaacgcg
     3241 gtcgtgggcg tccggcgcca agccgcggtc gccagggcgg cggccggttc caaggatgcc
     3301 gtccatgcat gaatgggtgg aacaggcgct gcgcttgctg gctcgcggcg agccggcggt
     3361 gctggtgacc gtcgccaagg tccaggggtc cacgccgcgt gaaccgggcg cccgcatgct
     3421 ggtggccgcg acccggcaat ggcagaccat aggtggtggc cacctcgaat ggcgcgcgct
     3481 gtccattgcc cgtctcatgc tccagaacaa gtcgcggcag cgccagatcg agcgcatggc
     3541 actgggtccc agcctggggc agtgctgcgg cggcgtcgcg acgctggcgt tcgagctgct
     3601 cggtcaggcc gacgcagact ggttgaatga cctgtccacc cgattgaaaa gcggccaggc
     3661 gtcggtgcgc accgtgctgt ttcccgccga tgtgtcagtg ccggctgtgt tgcccggcgt
     3721 gacactggcc gcgccggaag cggccggggt tgtggagcag cctggcgagt ccggcaccgt
     3781 gggccagcct ggcgaatcct gtgctctctg gcaggcgggc gggcaacaag gactggccga
     3841 ggtcatcgcg ccgcccaggt tcaacattgt gctgttcggc gccggccacg tcggccaggc
     3901 gctggtgcgc atcctgggca cgttgccttg cacggtgaca tgggtcgatg agcgcgatac
     3961 gcagttcccc gacgacattc cggccaacgt cacggtggaa gacaccgaca cgcccgaggt
     4021 ggtgatcgag caggcgccgc ccgacagcta tttcatcgtc atgacgcaca accacgcgct
     4081 cgaccagcat ctgtgcgagc atattttcaa gcgcaccgac tatgcctggt tcggcctgat
     4141 cggttcgcgc accaagcggc acaagttcgc gcaccgcctg ctggcccgcg gcgtgtccgc
     4201 ggagcgcttc gagaaaatgg tgtgccctat cgggatggcc ggcatcaccg gcaagcagcc
     4261 cgccgtcatc gccgtggcgg tggtggcaca gttgctggcc gagcatgagg cgcgggccaa
     4321 caccctgcag ccggatatct cggcaacgat aggcacgccg gtcaccccgg cacgataaca
     4381 atccgtccgc ccgcccgggc ggcgtcactg gaagagactg atgacatcga cacacgcatc
     4441 gcgcgcgccc gtcgcgcatg gcccgcgtac ggcctatcgg gcgcaattgc tgtatttcaa
     4501 gcaggaaggg ccgcagacct attccaccgt gcacctgacg gatggtttgc tagaggtcga
     4561 gaatggttgc gtggcccggg tgggcgacca cgccgcgctg gcccccggtc tgtcaccgga
     4621 cgtgcaagtg gtcgacctgc gcggcaaact gatcatgccg ggtttcattg atacccatat
     4681 ccactacccg cagaccgaca tgatcgcctc gcccgcgccc ggtctgctgc cgtggctgga
     4741 aaagtacaca ttcccgaccg agagacgctt cgaaaacccc gagtacgccg ccgacgtggc
     4801 gcgcttcttc ctggatgaac tcgaacgctg cggcacgacc acggcgctgg tctattgcac
     4861 ggtgcatccc ggctcggccg aggccttctt cgccgaaagc aatgcgcgca acctgcgcat
     4921 ggtggcgggc aaggtgatga tggaccggaa ctgcccggag ttcctgcgcg atacggcgga
     4981 atccggcgga cgcgacaccg aggccatcat ccagcgctgg cacggcaagg gacggcagat
     5041 ggccgcgctc acgccacgct tcgcccccac ctccaccccc gagcagctgg gggtatgcgg
     5101 cgaactggcg caacgctatg aagacgtctt catccagacc cacgttgccg agaacaccga
     5161 cgaggtgaag tgggcgcagg aactatttcc cggacaccgc agctatctgg acatctacga
     5221 tcactacggg ctgctgcgtc gccgcgcggt ctacggccac tgcgtctggc tcgacgacac
     5281 cgatcgccgg cgcatggccg agaccggcgc cgtcgccgcg cactgtccca cgtccaacca
     5341 attcctgggc agcggcctgt tcgacttcgc ggccgccgag gccacccata tgcccacgac
     5401 gctggccacc gatgtgggcg gcggctcgtc cttctcgatg ctgcagacca tgaacgaggc
     5461 acacaaagtg gcacgcctgt caggctacca cctgagcgcc gagcgcatgt tctacctggc
     5521 caccgagggc gccgcgcaag cgctcgatct gggcggtcgc atcggcacgc tggccgaggg
     5581 cgccgaagcc gacttcgtgg tgctggaccc ccaggccacg cccctgctgg cccgccgcac
     5641 ggcttccgcc gattcgctgg aagaactgct tttcgcgttc gccatgctgg gcgacgaccg
     5701 agccgtgcaa gccacctacg ccgcaggcca ttgcctgcgg acgcgggccg ccgcttgaga
     5761 aacagggccg cttgacatct tccgcatttc cgcaccgcaa gctgtctgtc ggagtctggg
     5821 cgggaccggc gtaaggcgtg cgccggatct ggctcgggaa ggcgggggca gtcgtcgggc
     5881 gctgtctgag caaaacggcc agtccattgg actgaccgtt ttgcgagttt cgcccgacgc
     5941 ccgcctggac gagacagatt cggatgaagc acgccgtgcc ggccccgccc agactccgac
     6001 agacagcgcc ttaagatagc aacggcggat attagcagca cacccattcc cgcaaacact
     6061 ggaacgaagc cccgcgttac cccgccgact ccgcatccac cctcaccccg cattcactaa
     6121 gcagccgacg caaccaacga tgcgcctcgg aggaatgatt gcgctcatgc cacaactgat
     6181 agaaacgcat cggcggaaac gcgatcggcg aagggataat ccgcaacggc agaatccggg
     6241 agtagaacaa cgcgaagtgc ttcgacgtcg tgaaaaccag atcagtgcca ggcagcagat
     6301 acggcgccag attgaacgac tgcacgatca cccgttcatc acgcgtgata cgctcggccg
     6361 ccaacgcgct gtctatcacc cccctctggc tgatcgaata cggcatcggc accacatgcg
     6421 ccgcgcggcg gtagtcatcc accgtcatgc ctttttcagc caacgtatgg ccagccgaca
     6481 ccagacagac aatgtcatcc tcgagcagca tcgacaaatg catgcggtcc ggcggttcag
     6541 gccaattgcc gatgaccaca tccagctcac cctcggccag cgaacgctcg aaatcgaact
     6601 ccggccccaa cgcgtgcaca gtcagagacg cctgcggtgc catggcccgc aactgctccg
     6661 acacacgtcc catgaagacg ggggcgaggt agtcgggcga accgatgcgg aagacctgct
     6721 tgctggattg cgcatcgaaa ccttccggct cggcggtcat gcgttcgatt tcggccaatg
     6781 ccgaccgggc atgcgaaacc agcgacagcg cccgctcggt gggcaccatg cccccttttt
     6841 cgcgaaccag aatggggtcg ccgagaatat cgcgcagatg gcgcagcgcc acgctgacag
     6901 cgggctgtga ctgattcagc ttgagcgcga cgcgtgacac gctgcgttcc gcgaccagca
     6961 tgcacaacac cttgagaaga taggtatcca acagctcatt gctgtgtcgg ctcgccattg
     7021 aatcgctcct gatccacgat actggtgtcc tgtttgactg attcggctac ttgtcttcgg
     7081 cgcctggatt cgccatgcat tcgaatacaa gtgtacgaac caatcaaaca agacaccaga
     7141 gccccgtcca tgccagttct gttggggcgg gtccggtctt caggccaact cgccccagat
     7201 gtgcacgcgg ctgatgccgc cgtcggggaa catgttgacc cgcacatggg tgacggggcc
     7261 gaggtcatgg atggactcgc cctcgaagaa gtgctggtga tccatctgcg ttttctgttc
     7321 gctcagcagt tcgggccaga acatcgcctg cgtcaccagg gattggtcgg tggattcggc
     7381 cacgctcgcc gcctggatcg atacgcggtc ggggtagttg cccttgaagt gcgccgtgtc
     7441 gatctcgatc ttgcgcacga tggcggcatg acccagttcg atcaggcacc agtccgagcc
     7501 cggttcgcgc cgacgccgcg tttcccagcc gtcccccatg ttgacaccgc gccccggcat
     7561 gatcagacgg aacggcacgc cgaagtgcgc atcgttgtag gccacgatgc gtccgccact
     7621 gaccagcgcg gacacttcat gcagggcttt cgggtcttgt ttttcccagt cgcaggcggg
     7681 ttggccgtag acgcggaagc gcgccacgcc gccgtctggg aagatgttca ggcgcacgtg
     7741 cgtccagacg cgcgcgtcat tgtcgatctc gacgaagtgg tgcgagtcgc ccgccaggga
     7801 ctgggcggcc acgatttgcg tccacaccgt cttgtcgtcg gggtcgccct cgcagcggca
     7861 ggcctgcacc gaggcggcgg gcgggaagtt gccggtgaaa tggctggtgt cgatgtcaag
     7921 gccgcggatc gcgccaggca ggcccagccg cacgacggcc cagtcatggc cgccattgcg
     7981 gcgccggcgg gtttcccagc cgtccatcca cttgccgtgg tcgtcgaact tgccgacgat
     8041 gaagacgggc tcggagggtt gcagcgcacg ctcggcggcg gcaaagaatt cgtccgagca
     8101 ggccacgacg cgcgagccga aatcggcgct ggccaggttc gcgcagcgcg tggcgaacgc
     8161 gggcggttcg acgaacgcgg cattgatgac agtgccggtg gggagattgg gttttgccat
     8221 ggtttcctct tcgtggtgcc cggcgaggct tgtgccagtc ctgccgggtg ggtgatcaat
     8281 cattcgggag atccgcgtag cggtgaatcc ggtgcgtggc gggtcggttg tcgcgtggga
     8341 agcaggtcgc gcgggcgtcc gggacaggga cgggcctgtc ttgaccgctg tcgtcgtgac
     8401 gtcagtttcc cccttgccag gggtggtgct cagcccagtg atgcgcgatc gcggcgcgcg
     8461 tggggatcca gacccggtcg tgcttctgga tgtagtccag gaagcgccgc agggcggcga
     8521 agcgcgccgg ccgccccgtc aggcggcaat gcaggccgat ggacagcatc ttgggggtgg
     8581 tctcgccttc ggcgtagagc acgtcgaacg catccttcag ataggtgaag aactgctcgc
     8641 cgctgttgaa cccctggggc gccgcgaagc gcatgtcgtt ggtgtccagg gtgtagggca
     8701 ccatgagctg cgggcgcgtc cgcccattgc ccagatccac ggacgtccag aacggcaggt
     8761 cgtcgccata gtagtcggcg tcgtacagga aaccgccttc ttcggcgacc aggcgcagcg
     8821 tgttggggct gtcacggccg gtgtaccagc cttcgggatg gtggccgatc agcttggtga
     8881 cgacctcgat gcagcgttgc atgtgctcgc gctcgatgct ttcctgaacg ttctggtaat
     8941 ggatccagcg gtagccgtga cacgcgattt catggccgag ttcgacgaag gcttgcgcga
     9001 cttccgggtt gcgctccagc gccatgccca cggcgaaaat ggtgagaggc agaccacgcc
     9061 gttcgaattc tcgcaagatg cgccagacac cggcgcgcga accatattcg tagatcgatt
     9121 ccatggacag gtgccggtcg ggataggccg cggcgccgat gatctctgac aggaactgtt
     9181 ctgaacccgg atcgccgttc agcacgcagt tttcgccgcc ttcttcgtag ttcagcacga
     9241 actgcacggc gacgcgcgcc ttgcccggcc agttcgcctg gggcggggtg cggccgtagc
     9301 cgatcatgtc gcgggggtag tctttctgag ccatggaaac tcctagatta acgtttgacg
     9361 ctgaaagcga gccagggatc ggacgcctgc gcgccctcgg gctggttcat gcggtgttcg
     9421 cagtcttcga ggtgatggcg catggcggcc ttggccccct gtgcgtcgtt gcccgcaagc
     9481 tgttcaagaa tgtcgagatg ttcaacataa gagcaggtgc tttccccagg cgtgtcgtag
     9541 tgggcgatga tgagcgaggt gcgggcgcac aggctgtgca gcatctcggt cagcaccgca
     9601 ttgcccagca ggtgggccat ttcaacgtga aaggcgtttg acagccggat ccagctgaca
     9661 cggtccccgc actcgaatgc cttgcgctcg cgctctacca tgtcgtgcag cggagcgagc
     9721 cgttccgcaa gatccggcag ggccagcaac atgtcgatgg tcatgcactc catcgcctgg
     9781 cgcatttcga acacgtcgcg ggtttccttg gcgctgggct gccagacgaa ggcgccccga
     9841 ttcggttcga tgaccacgat cttgtcgtgc gcaagctggg acagcacggt gcgcacggtg
     9901 gagcgcggac acgcgaagac cgagcacagg gtggcttctg tcaacttggc gccgggcagc
     9961 aggcgccgat ccatgacggc atcgaaaatg tcgtcgtaga cgcggtcgac ttcggacttc
    10021 accccgccgc gcacgttgcg ccaagaagac gcgcgggggg cttccaggtc ggcggataca
    10081 tctcgatgcg gattcaggtt gagcatggcg aaaaaatcag gactagcact gttgacaatt
    10141 attggcgaag caccgaaaat cgtcaacata aattgttcac aattattgtg accagctttg
    10201 aaggagacat ttcacatggg gaaaatttcc acccacgtcc tggacaccgt gcgcggcaaa
    10261 cccgccaagg gtgtgcgcat cgacctttac cgtgtcgagg cggaccaccg cgagttgctg
    10321 cgccagacgg ccaccaatca cgatggccgc tgcgacgaac cgctgctcca gggcgacgcg
    10381 ctgcggaaag gcgtgtacga actggtcttc catgcgggcg actatttcgc ggagctcggc
    10441 gtgccggccg acgagccccg cttcgtggac cagatcacgc tgcgcttcgg cgtggcgcat
    10501 cccgaccaga actaccacgt gccgctgttg gtcacgccgt ggacgtggtc cacctatcgt
    10561 ggcagctgag gctgccgacc cggccccttt ctaccaaaga gactattcat ggaagcattc
    10621 ctgtttgaat acggcaatct gctcctgcgc tggctgcacg tgatcgcggc catcgcctgg
    10681 atcggcgaat ccatctactt cgtcatgctg gacaacagcc tgcatccgcc acacggggcg
    10741 gaaagcaaga aacgcggcgt tttcggcgaa atgtgggcgg tgcatggcgg cgggttctat
    10801 cacaaccaga agtacctgac caaccccgcc gaactgcccg acgacctgca ctggtcgttc
    10861 tggaaggcct acaccacctg gctgtccggg ttcggcctgt tcttcatcat gtacctgctc
    10921 aagccggagt tctatctggt caa
//
LOCUS       DASTNK010000144        52160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1045365, whole genome shotgun sequence.
ACCESSION   DASTNK010000144 DASTNK010000000
VERSION     DASTNK010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 52160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52160
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045365"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1095)
                     /locus_tag="VFR20_10830"
     CDS             complement(88..1095)
                     /locus_tag="VFR20_10830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0231058.1"
                     /translation="MNLPEFSVLDLSPIVEGGSAAQSFQRTLDLARHSEQWGYHRYWL
                     AEHHGMPGIASAATSILIGHVAGGTHTIRVGAGGIMLPNHSPLVIAEQFGTLCSLYPG
                     RIDLGLGRAPGSDPATSRALRRNLDSDPDDFPRDVLELIDYFSQEPRQPIRAVPGAGL
                     DVPIWILGSSLFGAQLAAAFGLPYAFASHFAPAQMMQAIELYRNRFQPSPRLKKPYVM
                     LGFNIIAADTDQAAQRLATSVQQSFVASRTARPIALPPPRDNYLDELDPPRRALLDGI
                     LACSAIGSPETVAQRLGEFARQTRADELMIVSQIHDHSARLRSYEITADIRSRPCPLP
                     A"
     gene            complement(1092..4367)
                     /locus_tag="VFR20_10835"
     CDS             complement(1092..4367)
                     /locus_tag="VFR20_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU0231059.1"
                     /translation="MRFPLKPAGGIQARMFLILCIAVIPTFVGLGFYAYHNRDEIISL
                     SRNSTQNYVDLVARHESWLLRGYEQALRSIGATRVVQQRDWLECDRYISRLATPHSPY
                     VNVGVVGLDGQLLCSGRRPTAPKPGNVSDRSYFQRALRQPGFVVSDFLEGRFSHQPTL
                     VIATALELADGGPRVVLYAALDLKELSRAQHDVQLDKAARLTITDRNGTVLYSTQPTL
                     KPGQKLDDAPMRPDSTRDGPEATSLRDSRKWFVTRAPAGTDNDPSALTIVYEYPAAPL
                     VSAVDRELLIGVATLLIITLLALVAGWQSTQILIGRDIKHLVEAAKRLRKRDFSGKLA
                     GKVTGRELSDIAQQFDEMSEELQFHEKQWEAAVRKQTGQNTILRRVARNEPLNETLTA
                     LVTFVQEQAPPNVAAVVLLQPGTRQIALCLGPDLPPDYRNALIGCDASPLIHMNGPNA
                     DNTSISPGDVGTDPLWPGPSRLAPNDNLRACWSAPIISANDVLLGAFAFYRSNPHTPT
                     DEESRLGQTAAELAAVAIDRDRTSRALVQSEVEYRLLFETNPHPMWVYNAKTLRIMAV
                     NNRAIAHYGYSRGEFLRKRISDLIVDAGRPGEQEAAVPNLIAQTHRTKGKQDILVEIA
                     YFPLHFMGQQAILALINDITEQRQHSEALQYGAKHDLVTGLLNRVSFTAHVNDTIAAA
                     KSADAPVYLIVLSLNEFKEINTSLGYALGDALLKEAGERIQLVAGSAPVARMVSDEFA
                     ILLGPGTHSTTMLQTIDGLLAQVKRPFLLNDTQIQVTACIGIAQYPSDGQSMGLLLQH
                     ANSAMFQARQESSGYAFYDMANDHLAPERVLLASQLQRGLNEHRFELRYQPKVPLHEG
                     LPYGFEALVRWNSPDSGQVSPDKFISVVESSDLIHPFTHWVLNSAVEECMKWHAHGYH
                     ISVAANVSARNLLDSLLPERIKQILTRHALAPKYLELEITESSIMSNPERSLNVLRKI
                     HDIGVGISIDDFGTGYSSLAYLQKLPVDSLKIDRSFVVEMDREDDTRPIINSIIEMAH
                     SLGITVTAEGIESRQIMAKLAGMNCDFAQGYHISEPMAADNVLDWLKRNIPDTPLPTS
                     DTQ"
     gene            4728..5567
                     /locus_tag="VFR20_10840"
     CDS             4728..5567
                     /locus_tag="VFR20_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEU0231060.1"
                     /translation="MQQLLERIASLGPWVTQFGLNLILALVILVVGWVVSDLAGRAVH
                     KAAAHSSRIDPTVVPMAHSATVWTIRIFVIVAVLARFGVQTASIIAVLGAAGLALGLA
                     LQGTLQNIAAGIMLLALRPIRAGEFVSIVGKADGTIDEVGLFLSRLTQADGTHISLPN
                     SLVWGNPIVNYSRNKTRRLDFQIGVRYGDNLELALSELRGLVQAHELVLKDPEPQVMV
                     TEYRDNVIIVNIRAWSEAGRYWDLRFDLYRRAPEVLGRVGLQLPIPVREIQTVPSAGV
                     AAK"
     gene            complement(5628..6425)
                     /locus_tag="VFR20_10845"
     CDS             complement(5628..6425)
                     /locus_tag="VFR20_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU0231061.1"
                     /translation="MFPRVRFIMVRPSHPGNVGAAARALKTMGFDSLWLVAPRLDDVI
                     SHPDAVALASGATDVLSSARIVPTLADALAPVSLAFALTARARDLGPPPCDIREAAAL
                     GNTHLHSQPDSQIAVVLGTERTGLTNDEVALCERICHIPANPRYSSLNVAQALQLAAW
                     EFRYALAVAANLPLLPVTDAHARAGDQPASNLEVQSLLAHWERALVATGFLDPAHPKK
                     LMPRMRHLFMRSALSRDETDMLRGVCTAMIRSTHHDPGSTPGGSGNL"
     gene            6620..7411
                     /locus_tag="VFR20_10850"
     CDS             6620..7411
                     /locus_tag="VFR20_10850"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU0231062.1"
                     /translation="MHPMLNTAIKAARRAGTIINRASLDLERLQVARKGPKDYVTEVD
                     HAAEEAIIDVLRTAYPDHGFLAEESGTHGAPDAQGAGKPEFQWIIDPLDGTTNFIHGF
                     PMYAVSIALAQRGQVTQAVVYDPTRNELFTASRGSGAFLNDRRIRVSGQTRYADALIG
                     AHVPGSAGGGSVPARYASLLTECASARRVGSTVLDLAYVASGRCDGFCGVNLKPWDVA
                     AGALLVLEAGGLLADFNGEQSWLSSGEVLAASPKVFAQMMGYIQT"
     gene            7542..9113
                     /locus_tag="VFR20_10855"
     CDS             7542..9113
                     /locus_tag="VFR20_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEU0231063.1"
                     /translation="MEWVLEPAAWVGLFTLIVLEIVLGIDNLIFIAILIGKLPPAVRD
                     RARVIGLSLALVLRLLMLSAMSWLIKLTAPLVHWGSFGFSARDLILIIGGLFLVFKGT
                     VELHERLEGRTSVATGARLHAGFWVIVTQIVILDAVFSIDAVITAVGMVDHLAIMMLA
                     VIIAVGVMLVASKPLARFIGAHPTVVVLCLGFLLTIGFSLLAEGFHFPVPKGYLYAAI
                     GFSVIIEALNQMARRNVNRLESRRPMRERTAEGILRMLGKRPIAGPDEEPRHEPPEQV
                     FQDEERYMVSGVLTLADRSIHSIMTPRADVSWVNLDDDPAALRQQIAKAPHSFFPVCR
                     GALDEVIGIGRAKEMIADLITIGHVRLSRLRDPIIVHESIGVLRLMDTLKRSRGQLVL
                     VTDEFGTIEGVVTPIDVFEAIAGEFPDEDETPDIVTEGENRWRMDGAADLHHLELLLN
                     TDGLVDDEEEYSTLAGYLLERFGYLPRPGDTCELKQPHATYRFKVLKLDGRRIASVEV
                     ERLPPGPPDESDDGG"
     gene            complement(9183..9983)
                     /locus_tag="VFR20_10860"
     CDS             complement(9183..9983)
                     /locus_tag="VFR20_10860"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEU0231064.1"
                     /translation="MNKIRIPGAVWIASDVHLGPGTPRTRAAFFRFLEDAAREADALL
                     LCGDIFDAWIGDDLALRDPPPWLEDTIAHLKATAAALPLWLGRGNRDFLMGSELSNYL
                     GAHLLPDTCRLACDSGDILLSHGDEYCTDDKAYQRFRQVVRTPLAQGMFLSLGLRTRQ
                     RIAAWARRRSMAANRAKPAEIMDVSLQAVMNAFEQSGLDTMVHGHTHRPAVHTTEVGA
                     RTCTRFVLPDWHYEGADTRGGWLIIDREGVRLVQSPPLPPGTPPTGDS"
     gene            complement(9976..10485)
                     /locus_tag="VFR20_10865"
     CDS             complement(9976..10485)
                     /locus_tag="VFR20_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0231065.1"
                     /translation="MDTHPRVDLHTNMGDIVIELNPEKAPKTVANFLTYVKEGFYDGT
                     IFHRVINNFMIQGGGFEVGMKQKQTHAPIENEADNGLKNDKYTVAMARTSDPHSATAQ
                     FFINVANNEFLNFTAPTPNGWGYAVFGKVVEGTDVVDKIKQVKTGNKGFHQDVPVEDV
                     IIEKATVVE"
     gene            complement(10670..11422)
                     /locus_tag="VFR20_10870"
     CDS             complement(10670..11422)
                     /locus_tag="VFR20_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0231066.1"
                     /translation="MLRRFRSVIFVLALLASAAAAAQPAAGRLIARDFNLAASFQANG
                     TTAPEAATIEPQSCVTLPLQGAKLFETKTEDRGWKGLAHLLQAITPSVDTSVPLTASE
                     ITSRIATMLDQGQNQKALDIIERRLAQEQAAYRTGTDVQLMFLHARALAALGRDQEAE
                     TLYQHMTTQYPELPEPWNNLAAEYAKQGKLEMARDALRMALTADPNYAQAHANLGRVQ
                     LLLASQSFQDAAKLGVSGAQAKALKAQNALRP"
     gene            11533..12981
                     /gene="cysS"
                     /locus_tag="VFR20_10875"
     CDS             11533..12981
                     /gene="cysS"
                     /locus_tag="VFR20_10875"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU0231067.1"
                     /translation="MLHIYNTLSRAKEPLKTARPGCVRMYVCGITVYDFCHLGHARMM
                     VGFDVVQRWLRANGYDVNYVRNITDIDDKIIRRAVETNRRLGEVTRFYIDAMHADERA
                     LGVAPPDAEPRATEYVGDMLDIIGRLEDKGLAYRAEDGDVNYAVRGFPGYGKLSGKSL
                     DDLRAGERVAVAGSKHDPLDFVLWKAAKEGEPPESRWTSPYGPGRPGWHIECSAMSKA
                     LLGLPLDIHGGGPDLKFPHHENEIAQTEGAFDQTLASIWMHCGPMMVDADKMSKSLGN
                     FRTIRQTVAGTDALPDTLADYPVNVREAEMLRFFIVRNHYRSAQNYAPDNLFDAQSAL
                     DRLYQTLLNVPADAVEIDWDHPSAQAFRSAMDDDFNTAGAVAALFELSTEANRTGSSR
                     VSGLLRALGGLLGLLQRDPATYLQSPTRYLSGAPARASLDSGHVEALIAERAAAKNAR
                     DFQKADEIRAMLREAGIELEDKPNGTTQWRRA"
     gene            12992..13630
                     /locus_tag="VFR20_10880"
     CDS             12992..13630
                     /locus_tag="VFR20_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEU0231068.1"
                     /translation="MTDQPINGGKPDFWDEASAHLMGRDRILRKLIPRHSSEWLLPSA
                     TPFITLARAIIGQQISTSAANSVWERFTGQCGRRPSPSSVLEASADDLRAAGLSRRKV
                     EYVQDLALHFAEKKVRPARWNAMDDEAVVAELCAIRGIGRWTAEMFLIFSLQRPDVLP
                     LDDAGLLKAISLHYFSGEPVSRFEAREVAQAWSPWRTVATWYLWRSLDAAAL"
     gene            13675..14640
                     /locus_tag="VFR20_10885"
     CDS             13675..14640
                     /locus_tag="VFR20_10885"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEU0231069.1"
                     /translation="MRNTFLEFEQPLAELENKIEELRFVQTDSAVDISDELGRLQQKS
                     QALAKSIYAKLTPWQTALVARHPQRPYTFDYVRTIFTDFHEMHGDRMYADDLSIVGGL
                     ARFHGTPCMVIGHQKGRDTKERAMRNFGMPRPEGYRKAMRLMRLAEKFGVPVFTFIDT
                     PGAYPGVGAEERGQSEAIGHSIYAMAELKVPTVATIIGEGGSGGALAIAVGDVVMMLQ
                     YSTYSAISPEGCASILWRSADKAPVAAEAMAITAPRLKELGLVDRVVNEPVGGAHRDP
                     ATMSRQLSRALADSLRQLSGMTPEQLIEHRMTRLMSYGRFQEARN"
     gene            14612..15676
                     /gene="tilS"
                     /locus_tag="VFR20_10890"
     CDS             14612..15676
                     /gene="tilS"
                     /locus_tag="VFR20_10890"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU0231070.1"
                     /translation="MAASKRHATDVDAPRLSAFLTGALARALDALPGHPGSFAAGVSG
                     GVDSAMLAVHAAALARERGLNFHIFHVHHGLQEPSAQWQSQVHDLAQALRVPCHSRCI
                     RVDGASGKGIEAAAREARYATFVELAELVGIRHLLLAHHRDDQAETVLLRLLRGAGPS
                     GLAAMAPVSHRDGLTYVRPWLELARARILDAARRYGEHTGWQPVRDPTNHDDQYTRAA
                     VRERLVPDLNERWPGWQGVLVRHARLSAEARDVLDEVAARDLAGLDCVDDGASFSLRA
                     WRELSPARQALVLRHWLDRLGQRMPSEARLNDVMRQLRGLHALGHDRDMRVRHGAVWI
                     RCRAGRVFLDGAPGPCHENR"
     gene            15745..17031
                     /locus_tag="VFR20_10895"
     CDS             15745..17031
                     /locus_tag="VFR20_10895"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU0231071.1"
                     /translation="MSLIVHKYGGTSMGSIERIRNVATRVAKWQAAGHQVVVVPSAMS
                     GETNRLLGLAKEITPRPDARELDMLAATGEQASSALLAIALLSQGVAARSFTGWQVPV
                     RTDSAYTKARIQSIDGSLIRAELESGRVVIVTGFQGIDDEGNITTLGRGGSDTSAVAV
                     AAALGADECLIFTDVDGVYTTDPRVVPEARRMSVVSFEEMLEMASLGSKVLQIRSVEF
                     AGKYRVPVRVLSSLTDPLIPLEEEMSSGTLITFEEDHKMEAAVVSGIAFSRDEAKVTL
                     LAVPDTPGVAYSILGPVAAANIDVDMIVQNQSVHGTTDFSFTVNRNDFQRTLDLLNDQ
                     IVPAVGAREIVPDDKVCKVSIVGIGMRSHVGVASLMFRTLCEEGINIQMISTSEIKTS
                     VIINDKYMELAVRALHKAFGLDRESAGVPPVQDASA"
     gene            17087..17179
                     /locus_tag="VFR20_10900"
     tRNA            17087..17179
                     /locus_tag="VFR20_10900"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17121..17123,aa:Ser,seq:gct)
     gene            17583..17768
                     /locus_tag="VFR20_10905"
     CDS             17583..17768
                     /locus_tag="VFR20_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231072.1"
                     /translation="MKRIIQITSCGNGIAALCDDSTVWRLLPDGKWQQLPQIPELNRY
                     HISDEERAAKARQAEMT"
     gene            17928..18908
                     /gene="denD"
                     /locus_tag="VFR20_10910"
     CDS             17928..18908
                     /gene="denD"
                     /locus_tag="VFR20_10910"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HEU0231073.1"
                     /translation="MKVLITGGAGFLGQRLARRLLARGRLTDGAGREGAIDQLTLADV
                     VPASDFGDARVRVETGDIADPSFLKRLVDARTTSIFHLAAVVSGQAEADFDLGMRVNL
                     DAPRLLLEACRQSGHVPKVVFTSSVAVYGGTLPDGVRDSTALNPQSSYGTQKAIGELL
                     LNDYSRRGFVDGRALRLPTISIRPGKPNKAASSFASGILREPLNGGNAVCPVSAGLRL
                     WLSSPRRAVESLMAGHELPAASLGLNRCVNLPGLSVTVGEMLAALERVAGAEVAARVS
                     FQPDPVIEKIVASWPGAWDDSRAKALGLEGDSDFDAVIRAYIEDDLPTGD"
     gene            complement(18984..20531)
                     /locus_tag="VFR20_10915"
     CDS             complement(18984..20531)
                     /locus_tag="VFR20_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEU0231074.1"
                     /translation="MIEKSLMDLMYGFGIAFEPHNLMWSIFGVIAGNLIGVLPGLGAL
                     TAIAILLPLTYVMHPVAAILMLSGIFYGSMYGGAIGAILLNLPVHPPHAVACLDGYPL
                     TKQGRGGAALGVAMMASFVAASIGILIMVFCSPLLVDVAFKFGPAELFSIMLLGLLAG
                     STMAKGSAIKGVAMTLFGLLLGVVGTDVSTGTVRFSFGLMHLQDGVDLVALAMGLFGV
                     GEFLLSVNRMTTVGGGTKMRLRDMRPTRDELKKSFFPMLRGTAVGTLFGVLPGTGPTI
                     TTFVAYALERKISKTPERFGHGAIEGVASPEAASHSKTQVDFIPTMSLGIPGDAVMAL
                     ILGALLIQGIQPGPQLIVSHPDIFWGLIASFWVGNLLLVVLNVPLIGLWVKLLQVPYK
                     FLFPAALFFIAVGCFSTQNSLFDVGEVLLFGIIGAVLTYLDFSVAPILLGYVLGPMME
                     QNFRRALLLSRGDLATFIERPISAWFIGACALLIAAQIAAFCWKKARKPRQATITVSD
                     EIKRFAE"
     gene            complement(20528..20989)
                     /locus_tag="VFR20_10920"
     CDS             complement(20528..20989)
                     /locus_tag="VFR20_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEU0231075.1"
                     /translation="MHKGRINKDYYGGALMILLGLGAIAEGTSYRLGTLRHMGPGFFP
                     AAVGALLALTGIIIAVEGLGGKNVSEDGGRPEWRGWLCIIGGIVAFILLGKYTGLLPA
                     TFAIVFISALGDRKNTIKGAAILSVAMCAVAAIVFWWLLRLQFPLLAWGGS"
     gene            21148..21696
                     /locus_tag="VFR20_10925"
     CDS             21148..21696
                     /locus_tag="VFR20_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEU0231076.1"
                     /translation="MGTEFPGFSGPSASTEAPLEMLAACHLRIERQCATLGRLIAHLA
                     EHGVDADARQAAERVMRYFDTSAIQHHADEEVDLFPALIDSMAGSDAVCIREMTQGLA
                     AEHRELEARWRRLRGPLSEIAAGVMAPLASEDVAGFVDFYRRHLAREDQELLPMAQRL
                     LSDADLAGVGRAMRERRGIKEP"
     gene            complement(21782..22960)
                     /locus_tag="VFR20_10930"
     CDS             complement(21782..22960)
                     /locus_tag="VFR20_10930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEU0231077.1"
                     /translation="MPTLSKMTCVEDFRKLARRRVPRMFYDYADSGSWTEGTYRSNER
                     DFQRLKLRQRVAVDIEKRSLRSTMVGIDVAMPVAIAPTGLTGMQHADGEILGAKAAEK
                     FGIPFTLSTMSICSIEDIAAHTHKPFWFQLYVMRDKDFIARLIDRAKAANCSALVLTL
                     DLQVLGQRHKDIKNGLSTPPRPTLANLINLATKPQWCMGMLGTKRRTFGNIVGHAKGV
                     SDLSSLSSWTAEQFDPSLSWKDIEWIKERWGRKLILKGIMDADDARHAVDSGADAFIV
                     SNHGGRQLDGAPSSIAALPHIVEAVGKDIEVLMDGGIRGGQDVLKAIALGAKGTFIGR
                     SFLYSLGAFGEAGVTRCLELLANELDVTMAFCGRTDIHNVDRSVLLNPEIFDDPGRSS
                     "
     gene            complement(23097..24068)
                     /locus_tag="VFR20_10935"
     CDS             complement(23097..24068)
                     /locus_tag="VFR20_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEU0231078.1"
                     /translation="MPPHPSMPPPAETAKTPRRTALPYDYARVEAVVMDYVPQWRTRG
                     FDGVVAIARGGLVPATMAAAALDLPLFALSYARPGRHTAWFTAAQPAPASRLLLIEDI
                     AGRGTTLIDCLAFLRGAGHDVGIFTLAYDRESRLRPDYGQEIPPGRAAWFPWEREAIT
                     DAFAGTGNRPNEPLHHYASWAVDLDGVLLPDLPATDYARDLEDTLWRRDRLKPCASLP
                     ALDLGHVAIITGRPEPDRARTRAWLDRHGFHGPLAMRDPARHAVAQTTAHKADAILAG
                     RHTHYLESDAAQAIDISLRLRVARVFWWDGARAIAVVAGTPIDGLPA"
     gene            complement(24087..25067)
                     /locus_tag="VFR20_10940"
     CDS             complement(24087..25067)
                     /locus_tag="VFR20_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEU0231079.1"
                     /translation="MNIAIPDDYQDCVRTLNCFALLKSHDVRIFNDTVKAPDALAARL
                     IDAEALVLTRERTVVTRELLERLPRLRLISQTGKVAANIDVRACTDHGVVIMEGRGDP
                     SPTAELTWALILASRRYLLNEANRLRDGQWQGFLGQQLRGQRLGIWSYGRIGKMVAGF
                     GKAFDMRVWVWGRQGSTDRARADGIEVAPSREAFFADSDVISLHVRLNDDTRGLVTGD
                     DLARMKPSALFVNTSRAELVREGALEQALRHGVPGFAAVDVYEQEPVLGAAHPLLALP
                     NALCTPHIGFVERDNYELYYGTAFENLVRFAKGDTAGVLNPEALAHRPNA"
     gene            25212..26324
                     /locus_tag="VFR20_10945"
     CDS             25212..26324
                     /locus_tag="VFR20_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEU0231080.1"
                     /translation="MPSLFDPIAIGDLLLPNRIVMAPLTRNRAPDAVPTDLMATYYAQ
                     RASAGLIVTEATAISHQGQGYAQVPGLYSEAQLAGWRRVTDAVHQAGGRIVVQLWHVG
                     RVSHTSLQPDQDAPVAPSAIQAKAKTYLVHADGTGGFTPTSEPRALRLDELPGIVDDY
                     RRATRVAMRAGFDGVEVHAANGYLIDQFLRAGTNLRTDGYGGALENRARFLFDVLDAV
                     VAEAGGGRTAIRISPVTPSNDASDPNPQPLFNHVVGRLADYGLSYVHVIEGATGGARD
                     FRQGGDAFDYASLRRAYVAAGGRGAWMVNNGYDATLAKHAIENGDADLVAFGKLFIAN
                     PDLVRRLRDEANLNSADNATFYGGGAKGYIDYPSLA"
     gene            26376..27800
                     /locus_tag="VFR20_10950"
     CDS             26376..27800
                     /locus_tag="VFR20_10950"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0231081.1"
                     /translation="MKDKRDVPKADRRPGSTAEIKPIHRGMVFFIVALALFMMAVDAT
                     IVATVLHTLQNGLHTSVNWAGWTLTAYSFGFVLMLPVSGKLSERYGRRRVFLGSVITF
                     TVASLCCGLAGNIFALIVLRVVQAAGGAGFTPSATGIIVDYFGDARDRAVSLFGSIFP
                     VGSMVGPIFGGLFVSYWTWRGVFFVNVPIGIAVIVLALRHIPPDRPKQTRIRFGMDVL
                     GMGWMGIGLLAGMLAVSYLGEQGVHIWSPGFVFAVLIAVISVLGFFRHVHRRENPFIA
                     PHLIHGPGFGPVNLVNMIYSGITIGSVALIPLYATNRYGINALDSGTLLIAQGAAAIL
                     LSVMAALALRRTGHRLPIYTGGLVIVVGMLLLAVSPPSGFPPYAWLAVSAFLVGAGAG
                     ALNPATRNAGLQLAPEHSSTLAALRSLSLQIGSITTISIATAILTDSGNPGGAQAWVY
                     VVAALLLIVALPLIARVPEHRGSW"
     gene            complement(27954..28030)
                     /locus_tag="VFR20_10955"
     tRNA            complement(27954..28030)
                     /locus_tag="VFR20_10955"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27994..27996),aa:Arg,seq:acg)
     gene            28411..29229
                     /locus_tag="VFR20_10960"
     CDS             28411..29229
                     /locus_tag="VFR20_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0231082.1"
                     /translation="MKKESIITLVAPLIMGMAFSVSSPVATAAETHGKTSILVGADTT
                     FPPFETEKDGKVTGFDIDMIRAIAKAEHMAVKIKTMPFNGIIPSLQAGSIDVAVAGIT
                     IKKSRMQNVDFSDAYYKSGLSVLVKKDSKIKSFADLKGHVIATKKATSSVDYMTSHGI
                     DPKYIKEFQNIDVAYSALETGGADAVVFDNPVNVNFKTSHKNVKIVGPLLTGEYYGIA
                     ISKKKPELAAKINAGLAKIKKSGEYHHLFVKYFGGDVSGAVKGVEKPAAVAVTD"
     gene            29416..30084
                     /locus_tag="VFR20_10965"
     CDS             29416..30084
                     /locus_tag="VFR20_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU0231083.1"
                     /translation="MNVIWQNLPQILEGLRLTIQYSIVAIILMVAIGLIAALMRLSKI
                     GILRWIASVYVEIFRSTPLLVQLFFIVLGLPAILPVNEWFGQLFYPILAAAITLSLNE
                     GAYVTEIIRAGILGVDRGQKEAAASIAMTGFQTMRYIVLPQAFKRMIPPLVNQAAQTI
                     KDTSLLAPVGIIELVYKGEVVIASTFASFTIWGLIAVLYFIIIFSVSKCASYLERRLQ
                     VDKR"
     gene            30071..30793
                     /locus_tag="VFR20_10970"
     CDS             30071..30793
                     /locus_tag="VFR20_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU0231084.1"
                     /translation="MINVKDLHKSFGHNEILKGITNHVEEKEVVVVIGPSGSGKSTFL
                     RCLNGLETVTSGEIEIAGMKLTDPHTNIHKLRQHVGMVFQQFNLFKHLTILENITIGP
                     RKVRKMSVEESNKIARELLAKVGLSGKEPSYPDELSGGQQQRVAIARALAMGPNVMLF
                     DEPTSALDPEMVGEVLQVMKDLAVEGMTMVVVTHEMGFAREVGHRVIFMDGGYIVEEG
                     SPKDIFGNPQMDRTKNFLAKIL"
     gene            complement(30887..31489)
                     /locus_tag="VFR20_10975"
     CDS             complement(30887..31489)
                     /locus_tag="VFR20_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEU0231085.1"
                     /translation="MTPIASTPGAAAPPPHLAKLPRRFYDRDTQVVARELLGKLLVHQ
                     VNGVRRVGRIVEVEAYLGPDDLAAHTSKGLTARTKAMFGPPGHAYVYLVYGMHHCMNV
                     VTEPEGVGTAVLLRALEPLANLSTNTRGPGRLCKAMSIDLGHYGHDLCSDTLYIAQPP
                     NEEKPFTIVARPRVGVDYAGEWAAKPLRFYVEGNRFISRK"
     gene            31797..32006
                     /locus_tag="VFR20_10980"
     CDS             31797..32006
                     /locus_tag="VFR20_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEU0231086.1"
                     /translation="MQTETGIVKWFNNEKGFGFIKPDSGGKDLFAHHTEILGTGFKSL
                     EENQRVSFVTADGPKGPQAKSIQVI"
     gene            complement(32082..33185)
                     /locus_tag="VFR20_10985"
     CDS             complement(32082..33185)
                     /locus_tag="VFR20_10985"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEU0231087.1"
                     /translation="MSASYNDVFTRPGARIVPTPACPAHALAEFIRRLPKAELHLHIE
                     GTLEPELIFGLAARNHLTLPYDSVESLRAAYAFTDLQSFLDLYYAGASVLITEQDFYD
                     LTMAYVRRARADGIVHAEIMFDPQTHTARGVAMETIFAGMARALREARDHHGFTSYLL
                     MSFLRHLSEQQAFDTLRAALPLREQYADLWTGIGLDSSERGNPPEKFAQVFARCKELG
                     FHLVAHAGEEGPAAYVVDALDVLCVERIDHGVHSEDDPALMRRLIDQRVPLTVCPLSN
                     LKLRVVNDLSRHNLARMLRAGINVTLNSDDPAYFEGYLLDNYVASATAFALTPEEIRQ
                     LAANSLAASFLPAKDKARLASDLAHTMAKHPVP"
     gene            complement(33206..34039)
                     /locus_tag="VFR20_10990"
     CDS             complement(33206..34039)
                     /locus_tag="VFR20_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU0231088.1"
                     /translation="MLDRLRQQIPSLSAAERKAAEFVLQHPGDAAKSAVGDIARRAGV
                     SEPTVIRFCRSLGCRGWPDFKVRLTASLMSGIPYVHSSLSSGESTAALAGKVFDNAVS
                     ALLRARNDLDPERVDEAVALLARARRIEFHGLGNSGIVAADAQHKFFRYDLCTVAYSD
                     PHIQMMAASMLGPRDVLVAISHSGRSKDLLDAVATARRNDCPVIAITASNTPLAQSAD
                     VLLRADTQEDTELYSPMLSRLVHLVIVDLLALSVAMALGKDVSRVLERTKKSLKGKRT
                     P"
     gene            complement(34067..34744)
                     /gene="pgl"
                     /locus_tag="VFR20_10995"
     CDS             complement(34067..34744)
                     /gene="pgl"
                     /locus_tag="VFR20_10995"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEU0231089.1"
                     /translation="MLRTFDTNEGYVEALSARIAALLRAAITINGRACLAVSGGHSPI
                     ALFQALSQRSDVDWTRVLVRLVDERFVAPDNADSNEHLVRTYLLTNEARSARFTGLVC
                     DPANLDRSLEQANRQTEHLDLVVLGMGDDGHTASLFPRAAQLAQGLDPAQTGRYLHVT
                     PPAASHERISMTLRALLGASQLILAIAGQNKRQIYEQAASATTVDYPVSFVLNQTSTP
                     IDVYWHA"
     gene            complement(34747..36207)
                     /gene="zwf"
                     /locus_tag="VFR20_11000"
     CDS             complement(34747..36207)
                     /gene="zwf"
                     /locus_tag="VFR20_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU0231090.1"
                     /translation="MANTPSFDLIFFGGTGDLTLRKLLPALYQAHKAGTLHPDGRIFA
                     LGRRDIEQAGYLALVETHVKPGLGDSFDPLVWADFLKRIHFHRLDAGNAADYGQLVGA
                     LGGPGNRVTVCYLATAPDLFTTICTHLASVGLNHPNVRVVLEKPLGHDLASSNAINDE
                     VGRHFAENQIYRIDHYLGKESVQNLMAIRFGNALFEPLWRREWIDNVQITIAEQLGVE
                     ERGDFYDRTGALRDMLQNHLLQLLCIVAMEPPASLAEDAVRDEKLKVLKSLKPFTTED
                     VVARTVRGQYRSGAINGQPVVGYLDEHNIAPDSKTETFVALQAEIANWRWAGVPFFLR
                     TGKRLQERVAEIVINFRNVPHAVFPMPLNLHSANRLVIRLQPKESIQLYFLAKEPGDA
                     MHLQSTYLNLDFHTMSKARWADAYERLLLDVIRGKLALFMRRDELVQAWTWVEPIIEA
                     WEHHSIAPRTYTAGSWGPAASSSLLSRAGVSWHEET"
     gene            36550..37572
                     /locus_tag="VFR20_11005"
     CDS             36550..37572
                     /locus_tag="VFR20_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0231091.1"
                     /translation="MIKNSSRATIADVARHAEVSKATVSRFLNHRETLLSPEMATRVE
                     AAIRELGYSPSPMAQALKRGRSRLIGLVVADITNPYSVAVLRGAEEACREAGYLVMLF
                     NLGNESARESAGIRALSSYQVEGFILNTMGHDAGAVVETARQGKPVVLVDRLHHGLDV
                     DFVSLDNDQAVHLCARHLCEGGYRELLLVSEPLGTVSSRMQRLAAFQSFVQDPANAVS
                     GRGVECGEDDNRLVEALRALRGRAGTGIPAVISSNAVVTLRVVAAVASLGWTLGRDIG
                     LVGIDETEWAPYVGPGISTVSQPTGELGRLATHCLLERLEGLETPARTILLPGSLVVR
                     GSSSGQ"
     gene            37957..38628
                     /locus_tag="VFR20_11010"
     CDS             37957..38628
                     /locus_tag="VFR20_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU0231092.1"
                     /translation="MHYSFQFGPVFEAWPSLLQGTWNTIVLSFLAMLFGLMVAIVCAW
                     GKTSGPRVLRWVINAYIELIRNTPFLVQLFFFYFGLPAVGVRWSAYTAALVAMIVNLG
                     AYAAEIVRAGIESIPKGQIEAGLALNLSRVEIFRFIILKPALRAIYPALTSQFILLML
                     ASSVVSVISADDLTSIAVNLVSQTFRSFEIYIVVTVIYLLLTLAFSFLFRRLFLLGLN
                     YPDRR"
     gene            38628..39281
                     /locus_tag="VFR20_11015"
     CDS             38628..39281
                     /locus_tag="VFR20_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU0231093.1"
                     /translation="MRTFGYNEFLYVLEAARWTILLSLVAFVGGVVVGLIIALARTSD
                     SKLARTLAIGYIQLFRGTPLLLQLFLIFFGAPVLGWEINPWWAAGIALTLNSSAFLGE
                     IWRGCIEAIPQGQWEAADALGLSFIWKMRDVVLPQALKIAVPPTVGYLVQVVKGTSLA
                     AIIGFTELTRAGQIMNNVTFQPLWVFSVVALIYFVLCWPLSLLAAYLERRLSAGMAR"
     gene            39323..40135
                     /locus_tag="VFR20_11020"
     CDS             39323..40135
                     /locus_tag="VFR20_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0231094.1"
                     /translation="MYFSRKIRTFVGAAALVTLGSVALGTVSANAAETIDQVKQKGEL
                     TVGMLVDFPPYGILNSDNKPDGYDADVARLMAKDWGVKLKIVPVKGPNRIPYLLTGKV
                     DLLVASLAITPERAKQVQFSQPYSAAQIVLFARKGDKITSPKDLSGLRIGVARASTQD
                     VAVTKIAPKNANIRRFDDDASAMQALLSGQVDAIGCSTTVAAQIAKRDPGKFDAKFVL
                     KQQEMAVAMRKGEPKTLKAVNAFVEKNIKNGDLSKLYEKWLGTPLPDMKAAS"
     gene            40158..40940
                     /locus_tag="VFR20_11025"
     CDS             40158..40940
                     /locus_tag="VFR20_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU0231095.1"
                     /translation="MTDEQGVASGRSAPRGETIIEARAVDKWYGEFHVLKSVDLNVRA
                     GERIVICGPSGSGKSTLIRCLNGLEKVQKGHVMVDGIELTSGARQVEAVRQEVGMVFQ
                     QFNLFPHMTVLRNCMLAPMRSRGAGKAEAESLAMKYLTRVRIADQAHKYPSQLSGGQQ
                     QRVAIARALCMTPKIMLFDEPTSALDPEMVKEVLDTMIGLADDGMTMLCVTHEMGFAR
                     RVADRVIFMADGEIIEQAPPDQFFDNPQHQKARSFLGQILNS"
     gene            40960..41730
                     /locus_tag="VFR20_11030"
     CDS             40960..41730
                     /locus_tag="VFR20_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU0231096.1"
                     /translation="MSLARQIIVSLSSFGASNVRLHGQRRYVELCAEAGADGVEIRGE
                     LLNDPARELPGLASCLDGVGLRAVFSSPAMLWDRTGALDRDALELGLAAAATLRAPVL
                     KMSIGGYGQASRTSLVELQGALSDGKVELLIENDQTPRAGSVSSLRRFFEAADETGIH
                     LGMTFDMGNWHWVGESSLQAAGIFAPRVRYVHCKGVQRQPGRWVAVALQESSAPWRAV
                     LRAMPDLAPRAIEYPLVGEDVEAVTRGAIQDLRTLGDD"
     gene            41730..42674
                     /locus_tag="VFR20_11035"
     CDS             41730..42674
                     /locus_tag="VFR20_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU0231097.1"
                     /translation="MDPLLDVVTFGEAMMLLVADRPGPLESVPAFYKRTAGAETNVAV
                     GLARLGLRVGWVSRLGVDSMGRYLLAAMRAEGIDCSHVICDPTQRTGMQFKGQVVDGS
                     DPPVEYHRKGSAASHMQSDDIDVSWLCAARHLHSTGVFAAISENCLATTTRTMALMRA
                     AGRTISFDPNLRPTLWSTEDRMRECVNRLAFAADWVFPGFAEGLLLTGHDTPEQIAAF
                     YRGHGARLVVVKLGPDGAYFDGEQGQGYVVGCPVAKVVDTVGAGDGFAVGVISAMLEG
                     RTVRQAVERGAWIGARAVQVLGDTEGLPTRAELELAES"
     gene            42676..43641
                     /locus_tag="VFR20_11040"
     CDS             42676..43641
                     /locus_tag="VFR20_11040"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEU0231098.1"
                     /translation="MNRKQVLVFKALPADQLARLRHECDVVVGDPRVPQQKAAFQQAL
                     STVDGMIGASHRFDDALLDAAPNLKVISSVSVGVDNYPLASLKSRGIMLCHTPGVLTE
                     TVADLYFSMILATSRRIVELANLVREGRWTGSIQPDMFGWDVHGKTLGILGYGRIGQA
                     IARRAARGFDMQVLYHTRTQPSAGDADNRIMPASFKEVLSESDFVVVVLPLTGQTLGL
                     IGSAEFAMMKPGAIFINGARGAIVQETALLDALNRGTLRAAGLDVFMTEPLPADSPLR
                     THPKVVPLPHAGSATHETRHAMAEMATTNLLLALKGRPPLAVFDA"
     gene            complement(43812..44444)
                     /locus_tag="VFR20_11045"
     CDS             complement(43812..44444)
                     /locus_tag="VFR20_11045"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU0231099.1"
                     /translation="MNALELLQQSPVIPVIVIKDVDLAVDLAKALVAGGVRCLEITLR
                     SCVAIDAIRLISQEVPDAIVGVGTVRTAAQLEAASDAGARFAVSPGLTRELSAAARAS
                     GIPFLPGIATPSESMFAADQGYMVQKLFPAEAVGGVALLKSIYGPMPDVVFCPTGGIN
                     PRNARSYLALPNVQCVGGSWLTPDSVVTNKQWDAITQLAKEACALTGSHA"
     gene            complement(44530..46377)
                     /gene="edd"
                     /locus_tag="VFR20_11050"
     CDS             complement(44530..46377)
                     /gene="edd"
                     /locus_tag="VFR20_11050"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEU0231100.1"
                     /translation="MTMNPAVLNVTQRIRDRSQSTRRHYLERTRAAYGGKTRRSALGC
                     TNLAHAAAAMPATEKGRLHHGDTPNLAIVSSYNDMLSAHQPLGAYPTVIKQAAFAVGA
                     TAQFAGGVPAMCDGITQGQDGMELSLFSRDVIAMSTAISLSHQVFDAAVYLGVCDKIV
                     PGLLIGALSFGHLPAIFVPAGPMPSGIANADKAKTRQLFAQGKVGRAELLESESRSYH
                     SPGTCTFYGTANSNQMLMEIMGLHLPGSAFVPPGTALRDALTRAAAQRAVAISQQGDT
                     FTPVAEVVTEATIVNAIVGLLATGGSTNHTLHLVAIARAAGVIINWDDFAELSAVVPL
                     LARVYPNGPADVNHFHAAGGVGLVIAELLDAGLLHDDVRTCAGPGLRRYTQEPFLDGD
                     GRLAWRAGVRVTGDDSVIRSVRKPFSPDGGLKVLHGNLGRAVIKSSAVAPEHRVVQAP
                     ALVFHDQDDLLTAYRQGLLNRDFVAVIRFQGPAANGMPELHKLTPVLASLQDQGLHVA
                     LVTDGRMSGASGKIPAAIHVCPEALAGGPLAKVRDGDLIRLDATTGELSVEEGLDWPG
                     RKPVTADLHGRQVGLGRELFTSARQSVGSAEEGATLFGSPDSPTSANAM"
     gene            complement(46657..47031)
                     /locus_tag="VFR20_11055"
     CDS             complement(46657..47031)
                     /locus_tag="VFR20_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEU0231101.1"
                     /translation="MTDRHLIIVAALVLLVGVGAGAFGAHGLKRVLSPDMLAVWHTAV
                     TYQMFHGLGLLAIAVLGSRFGSPLLSSASIVMLIGIVLFSGSLYLLALTGTRWLGAIT
                     PLGGLGFIIAWAMVAIAAYRHA"
     gene            47190..48194
                     /locus_tag="VFR20_11060"
     CDS             47190..48194
                     /locus_tag="VFR20_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HEU0231102.1"
                     /translation="MAEFEQPSAVPEKAVIASVLYRTGQPPVDVPLEQVHRPSDEPRQ
                     LLWVGLREPELETLRLMGERLGLGARAIEEITERHVRPKILEFEHMTLVVAVTIEVEN
                     LRPVFGETQLLIGRNFLITVRRGAVAAHNSLRQYLEGVPELLGRGSDYVASMLLDLLA
                     DRYLAALTKFEATVEAIEQQFMLRGFGDVDVRKLYRLRRDLLRMHTAIFPMTEICRRL
                     ARVDTGHIDANCRGYFGEVADRVQRIDELINSLREALAFAFEAGSMLGQSQQTDITKK
                     LAAWAAILAVPTAVAGIYGMNFVSMPELKWHYGYPLMMGCTAVACAILYWRFRKARWL
                     "
     gene            48214..49869
                     /locus_tag="VFR20_11065"
     CDS             48214..49869
                     /locus_tag="VFR20_11065"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEU0231103.1"
                     /translation="MEWSTHQVTNQVPDLVGYNLFDADVALNDAVRREGAAAFEQNLR
                     DYGSRLGGVEVAELALRANQDRPKLETFDRLGHRIDQVRFDPAWHQLMGMAFEQGMHV
                     GVVTGSEGFQAGWPAARAALYLLHGQIEAGTLCPLTMTAAAAPALRDEPWFHDLAPHL
                     YSRRYDPRDMPVPEKAGVTIGMGMTEKQGGSDLRANATQAFPLEQGAERGAAFRLVGH
                     KWFFSSPTSDAHLVLAHCGGALSCFFVPRWTPDGTRNAVRIQRLKNKMGNASNASAEV
                     EFQDAFGILVGDPGRGIPILAEMATRTRLDCVLGSTALLRQALVQALHHARTRQAFGR
                     KLADHGLMRQVLADLALESEAATVLAMRLAGALEKRGSSAEERVWVRILTPAAKFWIC
                     KRAIQAVAECMEVWGGNGYIEDGPMPRLYRETPVNSIWEGSGNVMCLDVLRALNHDAD
                     AGRAFADTLVRDASVDRPLRARAERLVKRLRTPGANPQPWARHISREIVLLSQAVLLR
                     KHAPAPVADAFMQTRIHSEADGVFGSGMPVGAAETLLARSWPV"
     gene            49870..50505
                     /locus_tag="VFR20_11070"
     CDS             49870..50505
                     /locus_tag="VFR20_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEU0231104.1"
                     /translation="MNRFLNPLVRQLSRAGALHRRPAAWAVLAGVLVVAVGVASKAGF
                     VVSGQREAGASDKVAYTLAGRVVHVADGDTFTLLVDGRRERIRMASIDAPETRKNRER
                     PGQPMAQAAKQALAGLVAGKTLALSCFEHDRYGRNVCDVPLPDGSTANRELVAEGLAW
                     ANGEGQGRYMRDRRLPGLEAAAREARKGIWSEPSPVPPWVWRYQCWKHGQC"
     gene            50591..51064
                     /locus_tag="VFR20_11075"
     CDS             50591..51064
                     /locus_tag="VFR20_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEU0231105.1"
                     /translation="MSLKVFDLQCDSGHVFEGWFGSADSYESQRENGLLTCPVCNSHN
                     VVRKLSASRVNTGAGRDPAAGESRPAQAQAVAAPAGRQLAQLQAEMLRQIREIVRGAE
                     NVGPRFAEEARRMHEGEAHERAIRGTATEEERQELASEGIAVMPVPDFLDDDRLQ"
     gene            complement(51154..51711)
                     /gene="efp"
                     /locus_tag="VFR20_11080"
     CDS             complement(51154..51711)
                     /gene="efp"
                     /locus_tag="VFR20_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU0231106.1"
                     /translation="MKSAQELRVGNVVTVGSDALVVQKAEYNKSGRNAAVVKLKFKNL
                     LTGSGSESVVKADEKFDIVVLEKKECTYSYFADPMYVFMDAEYNQHEVEADSMGDALS
                     YLEEGMAVEVVFHDGRAISVDLPTIVVREITYTEPAVRGDTSGKVLKPAKTNTGMDIN
                     VPLFCEIGDKIEIDTRTGEYRSRVM"
     gene            complement(51804..>52160)
                     /gene="earP"
                     /locus_tag="VFR20_11085"
     CDS             complement(51804..>52160)
                     /gene="earP"
                     /locus_tag="VFR20_11085"
                     /inference="COORDINATES: protein motif:HMM:NF021598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEU0231107.1"
                     /translation="LLWSSDLNLVRGEDSLVRAIWAGKPLIWHIYPQDENTHMDKLDA
                     WLRLASYPAEADAGLRAWNAGDTPAFRDSLEQTLQPATWARWQAASGRWAGSLAQSSG
                     LAQALLAFCAKYRQTR"
BASE COUNT         9269 a        15436 c        17128 g        10327 t
ORIGIN      
        1 ttgccgagag ctcgcgcatg agcgccacgc cataggcttc agcaccaccg aagcgattgc
       61 cgaagcggtc gaccacgaac gcgagtttca ggcgggtagc gggcatggcc ggctgcggat
      121 gtccgcggtg atttcgtacg aacgcagccg tgccgaatgg tcgtgtatct gggagacgat
      181 catcaactcg tcggcccggg tctgcctggc gaattccccg agtcgttgcg cgactgtttc
      241 gggtgagccg atggccgagc acgccaggat gccgtccagc agcgcacgcc ggggcgggtc
      301 gagttcgtcg agatagttgt cacggggtgg cggcaacgcg atggggcggg cggtgcgtga
      361 cgccacgaac gactgctgca ccgacgtggc cagcctctgg gcggcctggt cggtgtccgc
      421 ggcgatgata ttgaaaccca gcatgacgta gggctttttc agccggggtg aaggttgaaa
      481 gcggttgcgg tagagctcga tggcctgcat catctgcgcg ggcgcgaagt gcgaggcgaa
      541 agcgtagggc aggccgaagg cggccgccag ttgcgcgccg aagaggctgg agccgagaat
      601 ccagatgggt acgtccagtc cggcgccggg aaccgcccga atcggttggc ggggctcctg
      661 ggagaaatag tcgatgagct ccagcacatc gcgcgggaaa tcgtccgggt cgctgtcgag
      721 gttgcggcgc agcgcacgcg acgtggccgg gtcggagccg ggggcgcggc ccagcccgag
      781 atcgatgcgc cccggataca gcgagcaaag cgtgccgaat tgctcggcga tgaccaatgg
      841 tgaatgattc ggcagcatga tgccgcccgc gcccactcgg atggtatgcg tgccgccggc
      901 cacgtggccg atcaggatcg aggtcgccgc actggcgata ccgggcattc cgtggtgctc
      961 ggccagccag taacggtgat acccccattg ctcgctgtgc cgggccagat ccagtgttcg
     1021 ctggaatgac tgtgcggcgc tgccgccctc gacgatgggg gataggtcga gtacggaaaa
     1081 ttctgggaga ttcattgcgt gtcggaggtt gggagtggcg tgtcggggat gttccgcttg
     1141 agccaatcga gcacgttgtc ggcggccatg ggctcggaga tatgatagcc ctgtgcgaag
     1201 tcgcaattca tgccggccag cttcgccatg atctggcggc tttctatccc ttccgccgtt
     1261 acggtgatgc ccaggctatg agccatttca atgatggagt tgatgatggg gcgcgtgtca
     1321 tcctcgcgat ccatctcgac gacgaatgaa cggtcgattt tcagactgtc cacaggcagc
     1381 ttctgcaggt acgccagcga cgaatagccg gtgccgaaat cgtcgatcga aatgcccacg
     1441 ccgatatcgt gaattttgcg caggacgttc aatgagcgtt ccgggttgga catgatcgag
     1501 ctttccgtga tttccagctc cagatacttg ggggccaggg catggcgggt gaggatctgt
     1561 ttgatgcgct ccggaagcag cgagtccagc aggttgcgcg ccgacacgtt ggccgccaca
     1621 gagatgtggt agccatgcgc gtgccatttc atgcactctt cgacagcgct gttcaacacc
     1681 caatgcgtga aggggtggat caggtcgctg ctttccacga cggagatgaa cttgtcgggg
     1741 gatacctgcc cgctgtccgg agagttccag cggaccaggg cctcgaaacc gtatggcagc
     1801 ccctcgtgaa gcgggacctt gggctgatag cgcagctcga agcgatgctc attcaggccg
     1861 cgctgcaatt gcgaggcaag gagcacgcgc tcgggagcca ggtgatcgtt cgccatgtcg
     1921 taaaacgcat agccggagct ttcctgccgg gcctggaaca tggccgaatt ggcgtgttgc
     1981 aacagcaagc ccatggattg gccatccgat gggtattgcg cgataccgat gcaggcggtg
     2041 acctggattt gggtgtcgtt gagcaggaac ggccgtttga cttgcgcgag caggccgtcg
     2101 atggtctgca gcatcgtggt ggagtgggtt ccgggtccga gcaggatggc gaactcgtcg
     2161 ctgaccatgc gtgccaccgg cgccgagccc gcaaccagct ggatgcgttc accggcttct
     2221 ttcagcaacg cgtcccccag ggcatagccc agtgacgtat tgatctcttt gaattcgttc
     2281 aggctcagca cgatcagata gaccggggca tcggccgatt tggccgcggc aatggtgtcg
     2341 ttgacgtggg ccgtgaacga cacccggttc agcaggccgg tgacgagatc gtgcttggct
     2401 ccgtattgca gtgcctcgct gtgctggcgc tgctctgtga tgtcgttgat cagtgccagt
     2461 atggcctgct ggcccatgaa atgcagcggg aagtaggcga tttcgacgag aatgtcctgt
     2521 tttcccttgg ttcgatgagt ctgtgctatc aggttgggca cggccgcctc ttgctcgccg
     2581 ggccgaccgg catccacgat caggtcggag atacgcttgc gcaggaattc tccccggctg
     2641 tatccgtagt gcgcgatggc gcggttgttc accgccatga tccgcagtgt tttcgcgttg
     2701 tagacccaca tggggtgggg gttggtttcg aacagcaacc ggtattccac ctcggactgc
     2761 accagagccc gggacgtgcg atcccggtcg atggccaccg ccgcgagttc cgcagcggtc
     2821 tggccgagcc gcgactcctc gtctgtggga gtgtgggggt tggagcgata aaacgcgaaa
     2881 gcgcccagca acacatcgtt cgccgaaatg atcggggccg accagcaggc gcgcaggttg
     2941 tcgttcggtg ccaggcggct cggaccgggc caaagcgggt cggttccgac atcgcccggg
     3001 ctgatcgatg tgttgtccgc gttcgggccg ttcatgtgga tcagcgggct tgcatcgcac
     3061 cctatcagtg cattccggta atccgggggt agatcggggc cgagacatag cgcgatctgg
     3121 cgggtgcccg gttgcaacag aacgacggcc gcgacgttcg ggggggcttg ctcctggacg
     3181 aacgtcacca gtgccgtcag ggtttcgttg agtggctcgt tgcgcgccac gcgccgcagg
     3241 atggtgtttt gcccggtttg tttgcgcacc gcggcttccc actgtttttc gtggaactgg
     3301 agctcttccg acatttcgtc gaattgctgg gcgatgtccg agagctcgcg gccggttacc
     3361 ttgccggcca gtttcccgct gaaatcgcgc ttgcgcagcc gcttggccgc ctcgaccagg
     3421 tgttttatgt cacgcccgat gagaatctgg gtgctttgcc agcccgcgac gagggcaagc
     3481 agggtgatga tcagcagcgt cgccacgcca atcaagagct cacgatccac ggcggacacc
     3541 agcggggccg ccgggtactc atacacgatc gtcagagcag atggatcgtt gtctgtgccg
     3601 gcgggcgccc gtgtgacgaa ccacttccgg ctatctcgga gggaggtcgc ctccggtccg
     3661 tcccgggtcg agtcgggacg cattggtgcg tcgtccagtt tctggccggg cttgagcgtg
     3721 ggctgcgtgc tgtagaggac tgtgccgttg cgatcggtga tggtcaggcg cgcggccttg
     3781 tccagctgga cgtcgtgttg cgcgcgcgac aattccttga gatccagcgc ggcgtacagc
     3841 acgacccggg ggccaccgtc tgccaattcg agggcggtgg cgatgacgag ggttggctga
     3901 tgggagaaac gaccttcgag aaaatcgctc accacgaacc cgggttggcg caaggcgcgc
     3961 tggaaatacg atcggtcgga cacattgccg ggtttgggag cggtcgggcg ccggcccgag
     4021 caaagcaatt ggccatccag gccgaccacg cccacgttca cgtaaggaga gtggggggtg
     4081 gcgagccggc tgatgtagcg atcacattcc agccagtccc gctgctggac gacacgtgtg
     4141 gccccgatgc tgcgcagtgc ttgttcgtat cctctgagca accagctctc gtgtcgcgcg
     4201 accaggtcca catagttctg cgtcgaattg cgtgagaggg agatgatttc gtcacggttg
     4261 tgataagcgt agaagcccaa tccgacgaat gtcgggatga cggcaatgca caggatgagg
     4321 aacatgcggg cctggatgcc gcccgcgggt ttcaggggga agcgcattcg ttgtctccgg
     4381 gcggctgttc atcgcgccat ctggtcaatt ttcgatcccg gtgtcgcaga tagctgattt
     4441 tcatcgggtt tttcaaccaa ttcccaggtt gtccgttgtg ttgtccacaa ttccagtgga
     4501 taagtttctt cgtggttctg gcacaaacaa cgcgcgcaac gattgcgttg cgcagccttg
     4561 cgatagccgc gactagcgct gcgacttggg gaatcgtccg cccctcgtgg cggagacaat
     4621 gcccgggtat tataaataaa gcgctcgccc atcgggctgt tccctggagc acccgcggtg
     4681 cctgcagcat agggcgattt cccgagaacc aggagtgaac aaacatcatg cagcaattat
     4741 tggaacgaat cgcgtctttg gggccgtggg tcacgcaatt tggcctcaac ctgatcctcg
     4801 cgctggtcat actagtggtg ggttgggtgg tttccgacct ggccggccgg gcggtgcaca
     4861 aggcggcagc ccattcgtcc cgcatcgatc ccactgtggt gccgatggct cattcggcca
     4921 cggtctggac aatacgcatc tttgtcatcg tcgctgtgct ggcgcggttc ggcgtgcaga
     4981 cggccagcat catcgcggta ctgggtgccg ccggcctggc cctgggactg gctttgcagg
     5041 gcaccttgca gaacatcgcg gccggcatca tgctgcttgc cttgcggccc atacgcgcgg
     5101 gcgaattcgt gtccattgtg ggaaaggcgg acggaacgat cgacgaagtc ggcctgttcc
     5161 tcagccgctt gacgcaagcg gatggcacgc acatttcgct gcccaatagc ctggtctggg
     5221 gcaaccccat tgtcaattac agccgcaaca agacgcggcg tctggatttt cagattggtg
     5281 tgcgctatgg cgacaatctg gaactggcgc tgtccgagct gcgcggcctg gtccaggccc
     5341 atgagctcgt cctgaaggat cccgaaccgc aagtcatggt cacggaatat cgcgacaacg
     5401 tcatcatcgt caatattcgc gcttggtccg aggcggggcg ttactgggac ctgcggtttg
     5461 atctgtatcg acgcgcgccc gaagtgctcg gacgagtggg gctgcagcta cccattccag
     5521 tgcgtgaaat ccagactgtg ccgtcggctg gggtggcggc caagtaatga atgtcggccg
     5581 acatggcgtt ccgtgaacat aaagcgtggc gggacaggga tattccctca gaggtttcct
     5641 gagccgccgg gcgtggaacc ggggtcatgg tgtgtcgacc gtatcatggc cgtgcagacg
     5701 ccgcgaagca tgtcggtttc gtcccggctc agcgcgctgc gcatgaacag gtggcgcatg
     5761 cgcggcatca gcttcttggg gtgggccgga tcaaggaatc ctgtggcaac cagcgctcgt
     5821 tcccaatgcg ccagcaggct ctggacctcg aggttcgatg cgggttgatc gccggctctg
     5881 gcgtgcgcgt cggtgacggg cagcagcggc aggttggcgg cgaccgccag cgcgtagcgg
     5941 aactcccacg ccgccagttg aagcgcctgc gcgacattca acgaactgta tcgcgggttg
     6001 gccggtatgt ggcagatgcg ctcgcaaagg gcgacctcgt cgttggtcag gccggtgcgc
     6061 tcggtgccca gcacgaccgc tatctggctg tcgggctggc tgtgcaaatg ggtgttgccc
     6121 agtgccgcgg cctcccggat atcgcagggc ggtggtccga gatcgcgggc acgggccgtc
     6181 agggcgaaag ccagtgacac gggtgccagc gcgtccgcca gggtgggcac gatgcgcgcc
     6241 gaagacagta cgtctgtggc gccactggcc aacgccacgg cgtccgggtg gctgatgaca
     6301 tcatccaggc ggggggccac cagccacagc gaatcgaacc ccatggtttt cagggcgcgc
     6361 gccgcggcgc caacattgcc cgggtggctg ggccggacca tgatgaaacg cacgcggggg
     6421 aacatatccg attcggaggg aggggggagg cgagaggtgt cgaaggtcgt catttgggcg
     6481 gagcacccga gtcatttaaa atgcaaggct caagtgtacg cggatcggtt ggcgcacgcg
     6541 ggaccagggg cctgatgtgg ccggctcttg gcgcctcggc cggcgtattg ctctttactt
     6601 cgctcgaaca cggaaaccta tgcatcccat gctcaatacc gccatcaagg cggcacgccg
     6661 cgcaggcacc atcatcaatc gcgccagcct cgatctggag cgcctgcagg tcgcgcgcaa
     6721 agggcccaag gattacgtca ccgaggttga tcacgcggca gaagaggcca tcatcgacgt
     6781 tttgcgcacc gcctatcctg atcacggttt tctggcggag gaatccggca cgcatggcgc
     6841 gccggatgcg caaggggcgg gcaagcccga gttccagtgg atcatcgatc cgctcgatgg
     6901 cacgaccaat ttcattcatg gttttcccat gtatgccgta tccatcgctt tggcgcaacg
     6961 cggccaggtc acccaggccg tggtctacga cccgacccgc aacgagctgt tcaccgcgag
     7021 tcgtggcagc ggtgcattcc tgaatgaccg gcgcattcgc gtgtcgggcc agacgcgtta
     7081 tgccgatgcc ctgatcggcg cccatgtgcc gggctcggcg ggagggggct ccgttcccgc
     7141 acggtatgca agcctgctga ccgaatgcgc gagcgcgcgc cgcgttggtt ccacggttct
     7201 ggatctggca tacgtggcca gtggacgctg cgatggtttc tgcggcgtca atctcaagcc
     7261 gtgggatgtt gccgcaggtg ccttgctcgt gctcgaagcg ggcgggctcc ttgccgattt
     7321 caatggcgag cagtcgtggc tgtcgagcgg cgaggtgttg gcggccagcc ccaaggtgtt
     7381 tgcccagatg atgggctaca tccagacttg aacttgaatc cccagacgac actcatgacc
     7441 actctcgatg gactgaccgt gggtggttcc gcggcccgct tccaccgctg tcgcgttcgg
     7501 gctccacgca tggtcagcca cggttcacgg agcctttgat gctggaatgg gttcttgagc
     7561 ctgccgcctg ggtcgggctg ttcacactga ttgtgctcga gatcgttctc gggatcgaca
     7621 atctgatctt catcgccatc ctgatcggca agctgcctcc cgcggtgcgc gaccgcgcgc
     7681 gcgtcatcgg cctgtcgttg gcgctggtcc tgcgcctgct gatgctttcg gccatgtcgt
     7741 ggctcatcaa gctgaccgcg ccgctggtgc attggggatc gttcggtttc tccgcgcgtg
     7801 acctgatcct gatcattggc gggctgttcc tggtgttcaa ggggacggtc gagctgcacg
     7861 agcgcctgga gggccgcacc agtgtggcga ccggggcccg gctgcatgcg ggcttctggg
     7921 tcatcgtgac ccagatcgtg attctggatg cggttttttc catcgatgcc gtgatcacgg
     7981 cggtcgggat ggtggatcac ctggccatca tgatgctggc cgtgatcatc gccgtggggg
     8041 tgatgctggt agcgtccaag ccgttggcgc ggttcatcgg ggcgcatccc accgtggtgg
     8101 tgttatgtct ggggttcctg ctgaccatcg ggttttcgct gcttgccgaa gggttccact
     8161 tccccgtccc caagggctac ttgtatgccg ccatcgggtt ctcggtgatc atcgaggcgc
     8221 tcaaccagat ggcccgtcgc aatgtcaatc gcctggagag ccgacgcccc atgcgggagc
     8281 gtacggccga agggatattg cgcatgctgg gtaagcgccc catcgccgga cccgatgaag
     8341 aaccccggca tgaaccgccc gagcaggtgt tccaggacga ggagcgttat atggtcagcg
     8401 gcgtgctgac gcttgccgat cgttccatac actccatcat gaccccgcgc gcggatgtgt
     8461 cgtgggtcaa cctggacgat gatccggccg cgttgcgcca acagatcgcc aaggcgccgc
     8521 acagcttctt cccggtgtgc cggggcgcgc tcgatgaggt catcggcata ggccgcgcaa
     8581 aggaaatgat cgccgacctg atcaccatcg gccatgtccg tctgtcgcgt ctgcgcgacc
     8641 ccatcattgt tcatgagtcc attggtgtgc tgcggctgat ggacacgctc aagcgttcgc
     8701 gcggccagct tgtgctggtg accgatgaat tcggaaccat cgagggtgtg gtgacgccga
     8761 tcgacgtttt tgaagccatt gccggggaat tccccgacga ggacgagacg cccgacatcg
     8821 ttaccgaagg cgagaaccgc tggcgcatgg atggcgccgc cgacctgcat catctggagc
     8881 tgttgctcaa cacggatggc ctggtggatg acgaagagga gtattccacg ctggcgggct
     8941 acctgctgga gcgtttcggc tatctaccga ggccgggtga cacgtgtgaa ctgaaacagc
     9001 cgcacgccac ctatcggttc aaggtgctca aactcgatgg gcgacgcatc gcctcggtgg
     9061 aagttgaacg gttgccgccc ggcccgcccg atgagagcga cgacggcggg taaggaattc
     9121 cagtctggaa aaggctgggg ttaccctgtc ttctatgtga agcccccggt cttggccgcg
     9181 tgtcagctgt cgccggtggg gggcgtcccg ggtggcagcg gtggcgactg aaccagccgg
     9241 acaccttccc ggtcgatgat cagccagccg ccacgcgtgt cggcgccttc atagtgccaa
     9301 tcggggagca cgaaacgggt acaggttcgc gcgcccacct ccgttgtgtg cacggcgggg
     9361 cggtgggtat ggccatgcac catggtgtcc agccccgact gttcaaaagc gttcatgacg
     9421 gcctggagcg agacatccat gatctccgct ggcttggccc ggttcgcggc catgctgcgg
     9481 cgtcgcgccc aggcagcgat acgctgcctg gtgcgcaggc ccagcgacag gaacatgcct
     9541 tgcgccaggg gtgtccgtac tacctggcgg aatctctggt acgccttgtc gtcggtgcag
     9601 tattcgtcgc catggctcag aaggatgtcg ccgctgtcgc aggccaggcg gcaggtatcg
     9661 ggcagaagat gggcgcccag gtaattcgat agctccgaac ccatcaggaa atcgcggttg
     9721 ccacgcccca gccagagggg tagggcggcc gcggtggcct tgagatgggc gatcgtgtct
     9781 tccaaccatg ggggcgggtc gcgcagcgcc agatcgtcgc cgatccaggc gtcgaaaatg
     9841 tcgccgcata gaagcagcgc gtcggcctcg cgggcggcgt cttcaagaaa gcggaagaat
     9901 gccgcgcggg tccggggtgt gccaggcccg aggtggacgt cggatgcgat ccagacggcc
     9961 cccggaatcc ggattttatt caacgaccgt tgctttttcg atgatcacgt cttcgacggg
    10021 aacgtcttga tggaagccct tgttgcccgt cttgacctgc ttgatcttgt cgaccacgtc
    10081 ggtgccttca accaccttgc cgaacacggc gtacccccag ccgttgggcg tgggggcggt
    10141 gaaattcagg aattcgttgt tggccacatt gatgaaaaac tgggccgtcg cggaatgggg
    10201 gtcgctggtg cgggccatgg ccaccgtgta cttgtcgttc ttcaggccat tgtcggcttc
    10261 gttctcgatg ggggcgtggg tctgtttttg tttcatgccg acttcaaaac cgccgccctg
    10321 gatcatgaaa ttattgatga cacgatgaaa gatcgtgccg tcgtagaacc cttccttgac
    10381 gtatgtcagg aagttcgcca cggttttggg cgctttttcg gggttgagct cgatgacgat
    10441 gtcgcccatg ttggtgtgaa ggtcgacgcg gggatgggtg tccatgactg aggtgccttg
    10501 tgttgatgag gaggtggcgg cccacgcggc ggagcctggg gccaggatgg ccaggaccgt
    10561 gcaaatggcg aggcccagat tgcgtgccga cgaggccagc acgcgcgagg agtggttcag
    10621 cagtggcatg atttggaatg ggtgagacat ggggaaggag aagccggcgt catggccgga
    10681 gcgcgttttg cgctttcagc gctttggctt gtgcgcccga cacgcccagc ttggccgcat
    10741 cctggaacga ttggctggcc agcaggagtt ggacgcgccc cagattggcg tgggcctggg
    10801 cgtaattggg gtcggccgtc agtgccatgc gcagggcgtc gcgcgccatt tccagcttgc
    10861 cttgtttggc gtattccgcc gccaggttgt tccagggctc gggaagttcc gggtattgtg
    10921 tggtcatgtg ctggtagagt gtttcggctt cttggtcgcg gcccagcgcg gccagggcgc
    10981 gcgcgtgcag gaacatgagc tgcacgtcgg tgccggtgcg atatgcggcc tgttcttgcg
    11041 ccagacgcct ttcgatgata tccagcgcct tctggttctg gccctggtcc agcatcgtgg
    11101 cgatgcggga ggtgatctcg gaggcggtca gcggcacact ggtgtcgacg ctgggtgtga
    11161 tggcctgcag caggtgcgca agccctttcc agccgcggtc ttccgtcttg gtttcgaaca
    11221 gtttggcgcc ttgcagcggc agtgtcacgc aggattgtgg ctcgatcgtg gccgcttcgg
    11281 gcgcggtggt gccgttggcc tggaacgagg ccgccaggtt gaagtcgcgc gcgatcaggc
    11341 ggccggcggc cggctgcgcc gccgcggcgg cggacgccag cagcgcaaga acgaaaataa
    11401 cggaacggaa acgtcgcaac acgcgtgcct gcaatgagaa agttgggtgt tttgcggtgc
    11461 aagacaggga ggaacgagcc cacttgctat actaatcgac caccatttta gccttgccta
    11521 agcctccacc gcatgctgca catttataac accctttccc gcgccaaaga gcccttgaaa
    11581 acggcccggc ctggttgcgt gcgcatgtac gtgtgcggca tcacggtcta cgacttctgc
    11641 cacctgggcc atgcgcgcat gatggtgggt ttcgacgtcg tgcagcgctg gttgcgggcc
    11701 aacggctacg acgtcaacta tgtgcgcaac atcaccgata tcgatgacaa gatcatccgc
    11761 cgggccgtgg aaacgaaccg acgcctgggt gaagtcacgc gcttttacat cgatgccatg
    11821 catgcggacg agcgcgccct gggcgtggcg ccgcccgatg cggaaccgcg cgccacggag
    11881 tacgtgggcg atatgctgga tatcatcggg cgcctggagg acaagggcct ggcctatcgc
    11941 gcggaggacg gcgacgtcaa ctatgccgtg cggggttttc cgggctacgg caagctgtct
    12001 ggcaagtcgc tcgacgatct gcgcgccggt gagcgcgtgg cggtggccgg ttccaagcac
    12061 gatcccctgg atttcgtgct gtggaaggcc gccaaagagg gtgaaccgcc ggaatcgcgc
    12121 tggacgtctc cttatgggcc tggccgcccc gggtggcaca tagagtgctc agccatgagc
    12181 aaggcactgc tgggcttgcc attggacatt catggaggcg gtcccgacct gaaattcccg
    12241 caccacgaga acgaaatcgc gcaaaccgaa ggcgctttcg atcagacgct ggcctcgatc
    12301 tggatgcatt gcggtccgat gatggtggac gccgacaaaa tgtccaagtc cctggggaat
    12361 ttccgcacca tccggcagac cgtcgcgggc accgacgcgt tgcctgacac gctggccgac
    12421 tacccggtga acgtgcgtga ggccgagatg ttgcggttct tcatcgtgcg caatcattac
    12481 cgcagcgcgc aaaactatgc gcccgacaac ctcttcgatg ctcaaagcgc gctcgatcgc
    12541 ctgtaccaga ctttgttgaa cgtgccggcc gatgccgtgg aaatcgactg ggatcatccg
    12601 tcggcccagg cgttccgctc ggccatggac gatgatttca acaccgcggg cgctgtcgcc
    12661 gccttgttcg agctgtctac cgaggcgaac cgaaccggtt catcccgcgt gtcgggattg
    12721 ctgcgggcgt tgggcgggtt gcttggtttg ctgcaacgcg atccggcgac ttacctgcaa
    12781 tcacccacac ggtatttgtc aggcgcgcca gcgcgggcgt ctctcgacag cggccatgtc
    12841 gaggccctca tcgccgaacg ggccgcggcc aaaaacgctc gcgactttca aaaggccgac
    12901 gagatccgcg ccatgttgcg cgaagcgggc atcgagctgg aagacaaacc caatggcacc
    12961 acgcaatggc gtcgagcata gggagggccc gatgacggat caaccaatca atggtggcaa
    13021 acctgatttc tgggacgagg cctcggcgca cctgatgggc cgggaccgca tcctgcgcaa
    13081 gctcattccc cgccattcgt ccgagtggtt gctgccgtcg gccacgcctt tcatcacgct
    13141 ggcccgcgcc atcatcggcc agcagatctc gaccagcgcg gccaattccg tctgggaacg
    13201 gttcaccggc cagtgcgggc gtcggcctag ccccagttcg gttctcgagg cgtccgccga
    13261 cgacctgcgc gccgcgggcc tttcccgtcg caaggtcgag tacgtccagg atcttgcgct
    13321 gcatttcgcc gagaaaaagg tgcgtccggc gcgctggaac gccatggacg acgaagccgt
    13381 cgtggccgaa ctgtgcgcca tccgcggcat cggtcgctgg acggctgaaa tgttcctgat
    13441 cttcagcctg cagcgccccg acgtcctgcc cttggacgac gccggcctgc tcaaggccat
    13501 ttccctgcat tacttcagcg gcgaacccgt gtcgcgcttc gaggcgcggg aggtggcaca
    13561 ggcctggtcc ccctggcgca cggtggccac ctggtatctg tggcgcagcc tcgatgcggc
    13621 cgctttgtga cgcagcgact aaaataacgc ttcttcttcg gaattccggt gcatatgcgt
    13681 aatacttttc tcgaatttga acaacccctc gccgagcttg agaacaagat cgaagagttg
    13741 cgcttcgtcc agaccgattc cgccgtggac atctccgacg agctggggcg tctgcaacag
    13801 aaaagccagg cgctggccaa aagcatctat gccaagctga ccccatggca gaccgcgctg
    13861 gtggcgcggc acccgcaacg accctacacg ttcgattacg tgcgcaccat cttcaccgat
    13921 ttccacgaaa tgcatggcga ccgcatgtac gccgacgatt tgtccatcgt gggtggcctg
    13981 gcgcgtttcc acggtacgcc ctgcatggtg atcggccacc agaaaggccg cgacacgaaa
    14041 gagcgcgcca tgcgcaattt cggcatgccc cgccccgaag gctatcgcaa ggccatgcgg
    14101 ctcatgcgtc tcgccgagaa gttcggggtt ccggtcttca cgttcatcga taccccgggg
    14161 gcctatcccg gcgttggcgc cgaggagcgc ggccagtccg aagccatcgg acacagcatt
    14221 tacgccatgg ccgaactcaa ggtgcccact gtcgccacca tcatcggcga gggtggctcc
    14281 ggcggggcgc tggccatcgc cgtgggcgac gtcgtcatga tgttgcaata ttccacgtat
    14341 tccgcgatct cgccggaagg ttgcgcctcc attctttggc gtagtgccga caaggcgccc
    14401 gtggccgccg aggcgatggc gatcacggcg ccgcgcctta aagagcttgg cctggtcgat
    14461 cgcgtcgtca atgagccggt gggtggcgcg catcgcgatc ccgccacgat gtcccggcaa
    14521 ctcagccgcg cgctggccga ttcgctgcgg caactctctg gcatgactcc cgaacagctc
    14581 atcgagcacc gcatgacgcg gttgatgtcc tatggccgct tccaagaggc acgcaactga
    14641 cgttgacgcg ccgcgtcttt ccgcattcct gaccggtgcg ctcgccaggg cgctggacgc
    14701 gcttccagga catcccggat cgttcgcggc cggtgtcagc ggcggtgtgg attccgccat
    14761 gcttgccgtg cacgccgcag ctttggcgcg cgagcgcggg ctcaacttcc atatctttca
    14821 tgtacaccac ggcttgcagg agccatcggc ccagtggcag tcgcaagtgc atgacttggc
    14881 gcaagccttg cgcgtgccat gccacagccg ctgcatacgg gtggacggcg cgtcggggaa
    14941 aggcatcgag gcggccgcgc gcgaggcgcg ttatgccact tttgtcgagc tcgctgagct
    15001 tgtggggatc cgacacctgc tgctggctca ccatcgcgac gatcaggccg aaaccgtgct
    15061 gttgcggttg ctgcggggcg ccggccccag cgggctggcg gccatggccc cggtatccca
    15121 tcgcgacggg ctgacttacg tgcgtccctg gctcgagctg gcgcgtgccc gcatcttgga
    15181 cgcggcgcgc cgctatggcg agcataccgg ttggcagcca gtgcgggatc cgaccaatca
    15241 cgacgatcag tacacgcgcg ccgcggtgcg ggagcgactg gtccctgatt tgaacgagcg
    15301 ttggccgggt tggcaaggcg tcttggtgcg ccatgcccgg ctgagcgcgg aggcgcgcga
    15361 cgtgttggac gaggtcgcgg cccgggacct ggccgggctc gattgtgtcg atgacggcgc
    15421 cagcttctct ctgcgggcct ggcgggaact gtccccggcg cggcaggccc tggtgttgcg
    15481 ccattggctt gatcgcctgg ggcaacggat gcccagcgag gcgcggctca acgacgtcat
    15541 gcgtcagttg cggggcctgc atgcgctggg gcatgatcgt gacatgcgtg tgcgacatgg
    15601 ggctgtgtgg atacgttgcc gggccggccg ggtttttctg gatggtgccc cgggtccatg
    15661 ccatgaaaac cggtaaaata aacaactttg cccctgccgg cgccacaatg ccggtttttt
    15721 cggctcattg atacagagta gaagatgtcc ctgattgtcc acaagtatgg cggcacgtcg
    15781 atgggctcga tagagcgcat ccgcaatgtg gccacgcgcg tcgccaaatg gcaggccgcg
    15841 ggccaccagg tggtggtggt gccctcggct atgtcgggtg aaaccaaccg gctcctgggg
    15901 cttgccaagg aaatcacgcc gcgccccgat gcccgcgaac tcgacatgct agccgccacc
    15961 ggcgagcagg ccagcagcgc gctgctggcc atcgccctgt tgagccaggg cgttgctgcg
    16021 cgcagcttca caggttggca ggtgcccgtg cgcacggatt cggcctatac caaggcgcgc
    16081 atccagtcca tagatgggtc cctgatccgg gccgagctcg aatcgggccg cgtggtcatc
    16141 gtcacagggt ttcaaggcat cgacgacgaa ggcaacatca ccacgctcgg ccgtggcggg
    16201 tctgacacct cggcggtggc ggtggcggcg gccctggggg ccgacgaatg ccttattttc
    16261 accgacgtcg acggtgtcta caccaccgac ccccgtgtcg ttcccgaggc acgccgcatg
    16321 tccgtggtgt cgttcgagga aatgctcgag atggcctcgc tcggctccaa ggtgctgcag
    16381 attcgttcgg tggaattcgc cggcaagtac cgtgttccgg tccgcgtgct ttcatcgctg
    16441 accgacccgt tgattccgct cgaagaagaa atgtcttcgg gcacattgat tacattcgag
    16501 gaagaccaca aaatggaagc cgccgtcgta tccggcatcg ccttcagccg cgacgaagcc
    16561 aaggtaaccc tgctcgccgt gcccgacacc ccgggcgtgg cgtattccat cctcgggccg
    16621 gtcgcggcgg ccaacatcga tgtcgacatg atcgtgcaga atcagtcggt gcatggcacg
    16681 actgatttct cgttcaccgt gaaccgcaac gatttccagc gcacgctgga cttgctgaac
    16741 gaccagatcg tccccgccgt cggcgcgcgt gaaatcgtcc ccgacgacaa ggtctgcaag
    16801 gtgtccatcg tgggcattgg catgcgcagc catgtgggcg tcgccagtct gatgttccgc
    16861 acgttgtgcg aagaaggcat caacatccag atgatcagca ccagcgagat caagacctcg
    16921 gtcatcatca acgacaaata catggaactg gccgtgcgcg cgttgcacaa agctttcggc
    16981 ctggaccgcg aaagcgctgg cgtaccgccg gttcaggacg cctcggcctg atggcggtgc
    17041 ccgacggggc ctgttctttc ttatgctaga attgcaggct tcttccggag acgtggccga
    17101 gtggttgaag gtactcccct gctaagggag catacggctt atacccgtat cgagggttcg
    17161 aatccctccg tctccgccag taagcagtct acagcaagct acaacagtat agaaagcccg
    17221 catgtttcct ggcattgcgg gctttttgtt gtctgtgtgc gtgtatggca atgtatggac
    17281 atacagcttt ttcgggggta ttcgacacaa gcaggcgacc aaagagcgac atgacgccga
    17341 gtttttccat cattacggtc ggccgtctgc agttgcggcg gctcggcgtc tggataagat
    17401 aaacgcagct cgggagcagg gagtgatctg aacttgcgcc ttgaagagcg aaccgcgcct
    17461 gagtccgggg tttatttttt tggcccggag tcttgcagcc tgaaatttgc tgcaaaaacc
    17521 atatcggatc ggcactatcg atatgtagtt aaatgaaggt gtccttgcaa taaggagctt
    17581 tcatgaaaag gatcattcaa attacgtcgt gcggcaatgg cattgcggcg ttgtgtgacg
    17641 acagtaccgt ttggcgtctg ttacctgatg ggaaatggca gcaattgcct cagatacctg
    17701 aactgaatcg ttaccacatc tctgacgagg agcgggcggc caaggccagg caggcagaga
    17761 tgacttgacc ggtggtctcc tcccgctgat tcgcctggcg gcacgctcgc gtatgggttc
    17821 gatggccggc gtgtcgcgcc ggatgcgggt cagatcgccg aattccagtc ggcgttggcc
    17881 tggatatctg atcgcagccc ccattttggg gctgacaagg gagaaaaatg aaagtactca
    17941 tcaccggtgg tgccggattc ctggggcagc gtctggcgcg tcgcctgctc gcacgcggca
    18001 ggctgaccga tggggcgggg cgggagggcg ccatcgatca gctcacactg gccgacgtgg
    18061 tgcccgcgtc cgatttcggt gatgcgcggg tgcgggtaga aaccggggat atcgccgatc
    18121 caagcttcct gaagcgcctc gtcgatgcgc ggacgacgtc gatctttcat ctggcggcgg
    18181 tggtcagtgg ccaggcggaa gccgatttcg atctgggcat gcgggtcaat ctcgatgccc
    18241 cgcgcctgct gctcgaggct tgccgacagt cggggcatgt gccgaaggtg gtgttcacca
    18301 gctcggtggc ggtctatggt ggcacgctgc ctgatggggt gcgggattcg accgcgctca
    18361 atccgcaatc gtcgtatggc acacaaaaag cgatcggcga actgttgctc aacgattatt
    18421 cgcgccgagg tttcgtcgat ggccgggcgt tgcgcctgcc caccatcagc atcaggccgg
    18481 gcaagcccaa caaggccgcc tcttcattcg ccagcggaat cctgcgtgag ccattgaatg
    18541 gcgggaatgc tgtctgtccc gtatcggccg gcctgcgtct ttggctgtcc tcgcctcggc
    18601 gcgcagtgga atccctgatg gcagggcatg agctgcccgc cgcgtcactc ggcctgaatc
    18661 gctgtgtgaa tttgcccggt ctgtcagtca cagtgggaga aatgctggcc gcgctggagc
    18721 gtgtggccgg tgccgaagtg gcggcgcggg taagctttca acccgatccc gtcatcgaga
    18781 agatcgtcgc gagttggccg ggtgcctggg acgattcgcg ggccaaggcg ttgggtctgg
    18841 aaggcgactc ggatttcgac gcggtcatcc gcgcctacat cgaagacgat ctgccgaccg
    18901 gcgattgaac gggccctcgc ggagccctgc aatcgtcggg ctgcggctgg cgccgggccg
    18961 gtatgtatgc cgaccgacgc ctgtcattcc gcgaaacgtt tgatctcatc actcacggta
    19021 atcgtggcct ggcgcggctt gcgggctttc ttccagcaga aggcggcgat ctgcgctgcg
    19081 atcagcaagg cgcaggcgcc gatgaaccat gcgctgatgg ggcgttcgat gaatgtcgcc
    19141 agatcgcctc gcgacaatag cagcgcgcgc cggaagttct gttccatcat cgggcccagc
    19201 acatagccga gcaggatggg cgcgaccgag aaatccaggt acgtcagcac ggcgccaata
    19261 atgccgaaca gcagcacctc gcccacgtcg aacaggctgt tctgtgtgct gaagcaaccc
    19321 accgcgatga agaacagcgc ggcggggaac aggaatttgt agggaacctg cagcagcttc
    19381 acccataatc cgatgagcgg cacgttcagc acgaccagca gcagattgcc cacccagaaa
    19441 ctggcgatca ggccccagaa gatgtcaggg tgggacacga tgagttgtgg cccgggctga
    19501 atcccttgta tgagcagcgc gcccaggatc agcgccatga ccgcgtcgcc cggaatgccc
    19561 aggctcatgg tggggatgaa gtccacctgt gtcttcgaat gcgaggcggc ttctggcgag
    19621 gccacgcctt ctatggcgcc gtggccaaag cgttccggcg ttttggatat cttgcgttcc
    19681 agcgcatagg ccacgaaggt ggtgatggtc gggccggtgc ctggcagcac gccaaacagc
    19741 gtacccacgg ccgtaccgcg cagcatggga aagaacgact tcttgagttc gtcccgggtc
    19801 gggcgcatgt cgcgcaggcg catcttcgtg ccaccgccca ccgtggtcat gcgattgacg
    19861 ctgagcagga attcgcccac accgaacagc cccatcgcca gcgccaccag gtcaacgccg
    19921 tcctgcaggt gcatcaggcc gaaggagaag cgcacggtgc ctgtgctgac gtcggtgccc
    19981 accacgccca gcaagaggcc gaacagcgtc atggccacgc ctttgatcgc cgaccccttg
    20041 gccatggtgg agccggccag caacccgagc agcatgatcg agaagagctc ggcaggcccg
    20101 aacttgaagg ccacgtcgac cagcagcggc gaacagaaca ccatgatcag gatgccgatg
    20161 gacgccgcca cgaacgaggc catcatggcc acgcccagcg ccgcgccgcc acgcccctgt
    20221 ttggtgagcg gatagccgtc caggcaggcc acggcatgcg gcggatgcac gggcaggttc
    20281 agcaggatgg cgccgatggc gccgccatac atcgagccgt agaaaatgcc ggagagcatc
    20341 aggatggcgg ccaccggatg catgacgtaa gtgagcggca gcaggatcgc gatggcggtc
    20401 aacgcgccca gcccgggcag cacgccaatg aggttgcccg caattacgcc gaagatggac
    20461 cacatcaggt tgtgcggctc gaacgcaatg ccgaagccgt acatcaggtc catgagcgat
    20521 ttttctatca tgacccgccc caggccagca acgggaactg cagtcgcagc agccaccaga
    20581 acacgatggc ggccacggcg cacatcgcca cggacagaat ggccgccccc ttgatggtgt
    20641 tcttgcggtc acccagcgcc gagatgaaca cgatcgcgaa ggtggccggc agcagacctg
    20701 tgtatttgcc cagcaggatg aaagccacga tcccgccgat gatgcacagc cagccgcgcc
    20761 actccgggcg ccccccgtct tcgctgacgt tcttgcctcc cagcccctcc acggcgatga
    20821 tgatgccggt gagggccagc aatgcgccaa ccgccgccgg gaagaagccc ggccccatgt
    20881 ggcgcagggt gcccagccgg tagctcgtgc cctcggcgat cgcgcccagg ccgaggagaa
    20941 tcatcagggc tccgccatag tagtctttgt tgatcctgcc cttgtgcatg ggctctcctg
    21001 gatatggtgg tgatatcagg cccgtattgt atccatcggc ttcgtctttc tgtttgcgca
    21061 ggatcgctgg atgcgacggc agggggctgg ctcggcacat ccgcgggtcg gggtgtgcca
    21121 caatgaaagc ttcaggagga cgtcttcatg ggtaccgagt ttcccggctt ttcaggacca
    21181 tcggccagca ccgaggcgcc gctcgaaatg ctggccgctt gtcatctgcg catcgagcgc
    21241 cagtgcgcca cgctggggcg cttgatcgcc cacctcgccg aacacggtgt cgacgctgac
    21301 gcgcgccagg ccgccgagcg cgtcatgcgg tatttcgata cgtccgccat ccagcaccac
    21361 gccgacgagg aagtcgacct gttccccgcg ttgatcgatt ccatggcggg ttccgatgca
    21421 gtctgcatcc gtgaaatgac acaagggctg gctgcggagc accgggaatt ggaagcgcgc
    21481 tggcggcgtc tgcgggggcc gctttcggag atcgcggcgg gcgtgatggc gcctctcgcc
    21541 agcgaggatg tggcgggctt cgttgacttt tatcgacgcc atctggcacg ggaggatcag
    21601 gagttgctgc ccatggcgca acgcctgctg tccgatgccg atctggccgg ggtcgggcgc
    21661 gccatgcggg agcggcgggg catcaaggag ccctgaaacg cgccagcccg gggtgcgccg
    21721 gcttttgaac tggtggaagg cgccccgcgg gatggctgga cgacatcccg cggccccggg
    21781 tctacgagga gcgcccggga tcgtcgaaga tttccggatt cagcagaacg gagcggtcca
    21841 cgttgtggat atctgttcta ccgcagaatg ccatggtgac gtcgagttca ttggccagga
    21901 gttccaggca acgcgtcacc cccgcttcgc cgaatgcccc cagtgaatac aggaatgaac
    21961 ggccgatgaa cgtgcccttg gcgcccaggg cgatggcctt gagcacgtcc tggccgcccc
    22021 gaatgccgcc gtccatcaac acttcgatgt ccttgccgac agcctcgacg atatgcggca
    22081 gcgcggcgat ggaagagggc gcgccgtcga gttgtcggcc accatggttg gacacgatga
    22141 acgcatcggc gccgctgtcg accgcgtgac gcgcgtcatc ggcgtccatg atgcccttga
    22201 gaatcagttt gcgcccccag cgttccttga tccattcgat gtccttccag ctcaggctgg
    22261 ggtcgaactg ttccgccgtc caggacgaga gcgacgatag atcgctgact cctttggcgt
    22321 gaccgacgat gttgccgaac gtgcgccgct tggtgccgag catgcccatg caccattgcg
    22381 gtttggtcgc caggttgatc aggttggcga gggtgggccg gggaggtgtg gacaggccgt
    22441 tcttgatgtc cttgtggcgc tgccccagta cctggagatc gagcgtcagc accaacgcgg
    22501 agcagttggc cgctttggcg cggtcgatca ggcgggcgat gaagtccttg tcgcgcatga
    22561 cgtacagctg gaaccagaac ggcttatgcg tgtgcgcggc gatgtcttcg atcgagcaga
    22621 tgctcatggt ggacagcgtg aacgggatgc cgaatttctc ggcggccttg gcgccgagga
    22681 tctcgccgtc ggcgtgctgc atgccggtca gccctgtggg ggcgatggcc acgggcatgg
    22741 ccacgtcgat acccaccatg gtgctgcgca ggctgcgttt ttctatgtcg accgcgacgc
    22801 gctggcgcag tttgagccgt tggaagtcgc gctcgttcga gcggtacgtg ccttccgtcc
    22861 aggagccgga atcagcgtag tcgtagaaca tccgcgggac gcggcgccgc gcgagcttgc
    22921 ggaagtcttc gacgcaggtc attttggata aggtaggcaa gagcttctcc cgggtgatgc
    22981 ttgtgcgggt aacgtggcca gacggcgcgc tgccgcgaaa aatgaataaa tcataatgga
    23041 ttgtatgcgg gtgtgtcgcg ccgcttcgct atccatgcca gcggcatgac gtggcctcac
    23101 gcaggcagac cgtcgatggg cgtgccggcc acaaccgcga tggcacgcgc gccgtcccac
    23161 cagaacacac gtgccaccct cagccgcagg gaaatatcaa tggcctgtgc cgcatcgctt
    23221 tccaggtagt gggtatggcg acccgccagg atggcgtcag ctttgtgcgc cgtggtttgc
    23281 gccacggcgt ggcgcgcagg atcgcgcatt gccagcgggc cgtggaagcc atgacggtca
    23341 agccaggctc gcgtgcgcgc gcggtcgggc tccggccggc ccgtgatgat cgccacgtgg
    23401 cccaggtcga gggcggggag cgacgcgcag ggtttcagtc gatcacgccg ccatagcgtg
    23461 tcttcgagat cccgcgcata gtcggtcgcc ggcaggtcgg gcagcaggac gccatccaga
    23521 tccacggccc aggacgcata gtggtgaagc ggctcgttcg gacggttgcc cgtgccggcg
    23581 aatgcgtcgg tgatggcttc gcgttcccag ggaaaccagg cggcgcgacc cggtggaatc
    23641 tcctgcccgt agtcgggacg caagcgtgac tctcgatcat aggccagggt gaaaatgccc
    23701 acatcgtggc cagcgccgcg caggaaagcc aggcaatcga tcagagtggt gccgcggccg
    23761 gcgatgtcct cgatgagcaa caggcggctg gctggtgccg gctgcgccgc cgtgaaccag
    23821 gccgtgtggc gcccgggcct ggcatacgac agggcgaaca aggggagatc cagcgcggcc
    23881 gcggccatgg tggcgggcac cagcccaccc cgggcgatcg cgacgacgcc gtcgaatccc
    23941 cgcgtgcgcc actgcgggac gtaatccatc accaccgcct cgaccctcgc gtagtcgtag
    24001 gggagggcgg tgcgccttgg cgtcttggcc gtctcggctg gaggcggcat ggatggatgc
    24061 gggggcatgc ccaggaatgc tgtgttctag gcgttggggc ggtgcgccag cgcttcggga
    24121 ttcaggacgc cggcggtatc gcccttggcg aaacgcacga ggttttcgaa cgccgtgccg
    24181 tagtacagct cgtagttgtc acgctcgacg aagccgatgt gcggggtgca taacgcgttg
    24241 ggcagcgcca gcaacgggtg cgcggcgccc agcaccggct cttgttcgta gacatccacg
    24301 gcggcgaagc ccgggacacc gtggcgcagg gcctgctcca gcgcaccctc ccgaacgagt
    24361 tccgcgcggc tggtgttgac gaacagtgcc gaaggcttca tgcgtgccag atcgtcgccg
    24421 gtgaccaggc cacgggtgtc atcattgagg cgcacgtgca ggctgatgac gtcgctgtcg
    24481 gcgaaaaatg cctcgcgcga aggcgcgacc tcgatgccat ccgcgcgtgc ccggtcggtg
    24541 gaaccctggc gtccccatac ccagacgcgc atgtcgaaag cttttccaaa gcccgccacc
    24601 atcttgccga ttcggccgta gctccagatg ccgagcctct ggccgcgcaa ttgctgtccc
    24661 aggaacccct gccactggcc gtcgcgcaga cggttggctt cattcagcag gtatcggcgg
    24721 ctggctagga tcagtgccca ggtaagctcc gccgtgggag aaggatcgcc ccgtccctcc
    24781 atgatgacga ccccgtgatc ggtgcaggcc cgcacgtcga tgttggcggc cactttgccg
    24841 gtttggctga tcagacgcag cctgggcagg cgttcgagca attcccgtgt gacgacggtg
    24901 cgctcgcggg tgagtaccag cgcttcggca tcgatgagtc gcgcggccaa ggcgtcaggg
    24961 gctttgacgg tgtcgttgaa gattcgcacg tcgtgcgact tgagcagcgc gaaacaattc
    25021 agggtgcgga cgcagtcctg atagtcgtcc ggtatggcaa tgttcatggc tactgatgcc
    25081 tctggcgcgg tggcggggtg gggttccgcg ttcgagtgta tcagagcgcc gtggcgccgc
    25141 gtgccagggc cccgatgcgc cgaatcatgg agagtcgtac tatttagcaa ttctgagagg
    25201 aaccctccac catgccaagc ttgttcgacc cgatcgctat cggggatctc cttcttccca
    25261 atcgcatcgt catggcgccg cttacgcgta atcgcgcgcc tgacgccgtg cccacagact
    25321 tgatggccac gtattacgcc caacgggctt cggcggggct cattgtcacg gaagccaccg
    25381 cgatttcgca ccagggacag ggctacgccc aggtgccagg cctgtattcc gaggcgcaac
    25441 tggccggctg gcgccgtgtg accgacgccg tgcatcaggc gggtggccgt attgtcgtgc
    25501 agttgtggca tgtcggacgt gtgtcgcata cgtctctgca gcctgatcag gacgcgccgg
    25561 tggcgccttc cgccattcaa gccaaggcga agacatatct ggtgcatgcc gacggcaccg
    25621 gtgggttcac acccacgtcc gaaccccggg ccctgcgtct cgatgagttg cctggcattg
    25681 tcgatgacta tcggcgtgcc acccgtgtcg ccatgcgggc gggtttcgat ggggtggaag
    25741 tccatgccgc gaatgggtac ctgattgacc agtttctgcg cgccggcacc aatcttcgga
    25801 cggacggcta tgggggggcg ctcgagaacc gtgcgcgttt cctgttcgac gtgctcgacg
    25861 cggtcgtggc ggaggcggga ggcggtcgga ccgcgatccg catctctccg gtgacaccct
    25921 ccaacgatgc aagtgatccg aacccgcaac cgctgttcaa tcatgtcgtc ggcagactgg
    25981 cggattatgg tctttcttac gtgcatgtga tcgagggcgc caccggtggc gcgcgcgact
    26041 ttcgtcaggg tggcgacgcc ttcgattacg catcgctgcg gcgggcctat gtggcggcgg
    26101 gcggtcgcgg ggcctggatg gtcaacaacg gttatgacgc caccctggcg aaacacgcta
    26161 tcgagaacgg tgatgccgat ctggtcgcct ttggaaagct gttcatcgcg aatccggatc
    26221 tggtgcggag gttgcgtgat gaggcaaacc tcaacagcgc ggacaacgcc acgttctatg
    26281 gtggcggggc gaaggggtac attgattatc cgtcgctggc ttgagtttca tgactccaat
    26341 gtttccatgt cgtggctttg gcctgtgcgg gttccatgaa agataagcgt gatgttccca
    26401 aggcggatcg gcggccaggc tcgacggctg aaatcaagcc tatccaccgc ggaatggtgt
    26461 tcttcatcgt ggcgctggcg ctgttcatga tggcggtgga cgccaccatt gtcgcgacgg
    26521 tgctgcatac cctgcagaac ggattgcata catcggtcaa ctgggccggg tggacgctca
    26581 cggcgtattc gttcggcttc gtcctgatgc tcccggtcag cggcaagctg agcgaacggt
    26641 atgggcgtcg cagggtgttt ctcggatcgg tcatcacttt caccgtggca tcactatgct
    26701 gcgggcttgc cggcaacatc ttcgcgctca tcgtgctgcg tgtggtacag gcggcgggag
    26761 gggcgggctt cacaccgtcg gcgacgggga tcatcgtcga ctactttggc gacgcccgtg
    26821 atcgagcggt cagcctgttc ggcagcatct ttcccgtcgg gtcgatggtc ggacccattt
    26881 tcggcgggct gttcgtatcg tactggacct ggcggggtgt gttcttcgtc aacgtgccga
    26941 tcggaatcgc cgtcattgtg ttggcgttgc gtcacatacc gccagatcgc cccaagcaga
    27001 cacggatccg tttcgggatg gacgtccttg gcatggggtg gatggggatc ggcctgcttg
    27061 cgggcatgct tgccgtcagt tacctgggtg agcaaggcgt tcacatctgg tcgcctggat
    27121 tcgtgttcgc ggtgttgatc gcggtgatct cggttttagg gttcttccgc catgttcacc
    27181 gccgcgaaaa cccgtttatc gcgccgcacc tgatccacgg gcccgggttt ggcccggtga
    27241 acctcgtcaa catgatctac agcggcatca ccatcggttc ggtggcgctc atcccgttgt
    27301 atgcgaccaa tcgctacggc atcaatgccc tcgactcagg cactttgctt atcgcccagg
    27361 gggccgcggc catcctcttg tcggtcatgg cagccttggc gctgcggcgc accggtcatc
    27421 ggcttcccat atacacgggg ggtctggtca tcgtggtggg tatgttgttg ctggctgtga
    27481 gcccgccatc cggctttccg ccatatgcct ggctggcggt gtcggcgttt ctggtgggcg
    27541 cgggggccgg agcgctcaat ccggccaccc gcaatgcggg gctgcaattg gcgcccgagc
    27601 attcgtcgac tctcgcggct ttgcgctccc tgtcactgca gatcgggtcc atcaccacga
    27661 tctcgatcgc cacggcgatt ctcaccgatt cgggcaaccc gggaggggcg caggcctggg
    27721 tctatgtggt ggccgccttg ctgctgattg tcgccctgcc gttgatcgcc cgggtgcctg
    27781 agcatcgggg ctcatggtag cccgcgcccg atgtgcgaaa acgcggtggg tgggtggcgt
    27841 atcttgttgg cgtgccccgc tggggtgcgt cgctggtgtg cgtcgctgac atgccaactg
    27901 accacggaat ggacaagggc ttacagacgg aatctgtaag cccttgattt atatggcgcg
    27961 tctggcagga ttcgaaccca cgaccccttg gttcgtagcc aagtactcta tccaactgag
    28021 ctacagacgc gttgaaagag gcaaaactat aacacagttt ttccatggtg tcccgtgttc
    28081 attctgcgtc atcgtgaaac gtcgctggcg gcagcggggg aaccggctgg attttcctgt
    28141 tgttgtggtc gttcaacctt gttgatgagg cctgcccgat cggggtttcg catgcggccg
    28201 cgcaaattgt tcatcttgtt gtaagcaagg tttcatgcgg ctttcggaga ttccctgacc
    28261 gctaccatgg gtgaaagtcc gtcttgacgg cgcaggctcc ccgccggcat actaccccgg
    28321 caatcaagtg cattaatgtg catgaatgtt cttcaaattg ttccggcccg caaacaggcc
    28381 gtgttcgtcc aagtcttaag gagacacctc ttgaagaagg aatcaatcat cacgctggtc
    28441 gcgccgttga tcatgggcat ggcgttttcc gtcagctctc cggtggcgac ggcggcggaa
    28501 acacacggca agacatcgat tctggtgggt gccgacacca cgttcccgcc ttttgaaaca
    28561 gaaaaggacg gcaaggtcac cggtttcgac atcgacatga tccgcgccat cgccaaggcc
    28621 gagcacatgg cggtcaagat caagaccatg ccgttcaacg gcatcattcc cagcctgcag
    28681 gctgggtcca tcgatgtcgc ggtggcgggt atcaccatca agaaaagccg catgcagaac
    28741 gtcgatttca gcgatgctta ttacaagtcc ggcctgtcgg tgctggtcaa gaaagactcc
    28801 aagatcaaaa gcttcgccga cctgaagggc catgtgatcg ccacgaagaa agccacgtcg
    28861 tcggttgact acatgaccag ccatggcatc gatcccaagt acatcaagga attccagaac
    28921 atcgacgtgg cctattccgc tcttgaaacc ggcggcgcgg acgcggtcgt cttcgacaac
    28981 cccgtcaacg tgaatttcaa gaccagccac aagaacgtga agatcgtggg gcccctgctt
    29041 accggcgaat actatggcat cgccatcagc aagaaaaagc ccgaactggc tgccaagatc
    29101 aacgccggcc tggccaagat caagaaaagc ggcgaatacc accatctgtt cgtgaagtac
    29161 ttcggtggcg acgtcagcgg cgctgtgaag ggtgttgaaa agcccgctgc cgtggcggtg
    29221 accgactaag cgtcttcata tttgaagatg cctggaatgg ccgactcgta ccaagcttgg
    29281 cacctcgggc ccgactatat tgttcgggcc gggtgccggg cgccatgagc cggaacgcgc
    29341 ggtacgcacc gcgcgttttg ttttcggact cgggcctgcc tccacgtcgg tcaacaatga
    29401 aataagacaa ggcttatgaa tgtaatctgg cagaatctcc cgcaaatact ggaaggcctg
    29461 cgcctgacca tccagtactc gatcgtggcc atcatcctca tggtggccat cggtctcatc
    29521 gccgcgctca tgcggctgtc gaaaatcggc atcctgcgct ggatcgcgtc ggtctacgtc
    29581 gagatcttcc gttccacgcc actgctggtc cagcttttct tcatcgtgct gggtctgccg
    29641 gccatcctgc cggtcaacga atggttcggt cagttgttct accccattct tgccgcggcc
    29701 atcacgctca gcctgaacga aggcgcctac gtcaccgaga tcatccgcgc gggcatcctg
    29761 ggcgtcgatc gtggccagaa agaagcggcg gccagcatcg ccatgacggg gttccagacg
    29821 atgcgctaca tcgtcctgcc ccaggcgttc aagcgcatga ttccgccgct ggtcaaccag
    29881 gcggcgcaaa ccatcaagga cacctcgctg ctggcgccgg taggcatcat cgagctcgtc
    29941 tacaagggcg aagtcgtcat tgcctccact ttcgcgtcgt tcacgatctg ggggctcatc
    30001 gccgttctgt atttcatcat catcttctcc gtgtcgaaat gcgcgtccta cctggagagg
    30061 aggcttcaag ttgataaacg ttaaagatct gcataaatcg ttcggccaca acgagatcct
    30121 gaagggcatc accaaccacg tcgaggaaaa ggaagtcgtc gtcgtcatag gcccgtccgg
    30181 cagcggcaag agcacgttcc tgcgttgtct gaacggtctg gaaaccgtca ccagcggcga
    30241 aatcgaaatc gccggcatga agctcactga cccgcacacc aacatccaca agctgcgcca
    30301 gcacgtgggc atggtgttcc agcagttcaa tcttttcaaa cacctcacca tcctcgaaaa
    30361 catcaccatc gggccgcgga aggtcaggaa gatgtcggtc gaagagagca acaagatcgc
    30421 ccgcgagctg ttggccaagg ttggtctgtc cggcaaggaa cccagctacc cggatgaact
    30481 ctccgggggg cagcagcaac gcgtggctat cgcgcgggcc ctggcgatgg ggcccaacgt
    30541 catgttgttc gacgaaccca cttcggcgct tgatccggag atggttgggg aagtgctgca
    30601 ggtcatgaaa gacctggcgg tcgaaggcat gaccatggtg gtcgtgactc acgaaatggg
    30661 gttcgcgcgc gaggtggggc accgggtcat cttcatggat ggtggctaca tagtcgaaga
    30721 aggatccccc aaggacattt tcggcaatcc gcaaatggat cgcaccaaga atttcctggc
    30781 caagattctc tagagagttc gtggtggcgg catgcgtggg cgttggccga cccaccgcaa
    30841 tgctcactca gaaatggcac cgcgtcggcg tatggccgac gtgacgctat ttcctcgaga
    30901 tgaacctgtt gccttccacg tagaaacgca agggcttggc cgcccattct cctgcgtaat
    30961 ccacccccac gcgaggacgc gcgacgatgg tgaagggttt ctcttcgttc ggcggctgtg
    31021 cgatatacaa ggtatcgctg cacagatcat gcccgtaatg gcccagatcg atgctcatgg
    31081 ccttgcacag acgcccgggg ccgcgggtgt tggttgacag gttggcaagt ggttccaatg
    31141 cgcgcaacag cacggccgtg cccacgcctt cgggctcggt gacgacgttc atgcagtgat
    31201 gcatgccgta aaccagatac acgtaagcgt ggcctggcgg gccgaacatg gctttggtgc
    31261 gggcggtcag ccccttcgac gtatgggcgg cgagatcgtc cggccccaga taggcctcta
    31321 cttcgacaat acgccccacg cgccgcacgc cgttcacttg atgcaccagc agtttgccca
    31381 acagctcccg agccacaacc tgtgtgtcac ggtcgtagaa tcgccgaggc agtttcgcca
    31441 ggtgtggagg tggggcagcg gctccggggg tggatgcgat gggggtcatg ggcctacgcg
    31501 gggcgttgtg aaaaccgggg tgtccatcat agtgcggttc acgcggtggc gccgcacacc
    31561 acgtgccggt gtcgggggga tgcgcccaac ccacccactc ccaatatgcc gcctatttta
    31621 cgaacttgac tttaaaatac agcaaaacta aaatttacgg tcagattttc aagcttgttg
    31681 atgttggttc aatccagtac cggcggttca gtactttttt gctcttatct ctgtcttgac
    31741 aagtctgtga cccagggcta tttcgccctt taaaaactct cagggattgc aaaaaaatgc
    31801 aaacagaaac cggtatcgtc aaatggttca acaacgaaaa gggctttggc ttcatcaagc
    31861 ctgattcggg cggcaaggac ttgttcgccc accacaccga aattcttggc acgggcttca
    31921 agtcgctgga agaaaaccag cgcgtgtcgt tcgtcaccgc tgacggcccc aagggcccgc
    31981 aggccaagtc gatccaggtc atctaaggta aggccgggtt aatccggcgt taccctgtac
    32041 ctgtttcttc gatgaaaaag ccacccaaaa gggtggcttt ttcatgggac ggggtgtttt
    32101 gccatggtgt gggccaggtc gcttgccaat ctggctttgt ccttggccgg caggaatgaa
    32161 gccgccagac tgttggcggc caattggcgg atttcctcgg gggtcagggc gaacgccgtg
    32221 gcgctggcga cgtagttgtc cagcagatag ccctcgaaat aagccgggtc atcggagttc
    32281 agggtgacgt tgatcccggc gcgcagcatg cgcgccaggt tgtggcggga gaggtcgttc
    32341 accacgcgca gtttcaggtt cgacagcgga cacaccgtca gtggcacgcg ctggtcgatg
    32401 agacgccgca tcagcgccgg gtcgtcttca ctgtgcacgc catggtcgat gcgttcgacg
    32461 cacagcacat ccagagcgtc gaccacatag gcagcgggcc cttcctcgcc cgcgtgggcc
    32521 actagatgga agcccaactc cttgcaacgc gcgaatacct gggcgaattt ttcaggtgga
    32581 ttgccgcgct cggatgaatc caggccgatg cctgtccaca gatcggcgta ctgttcgcgc
    32641 agaggcagcg ccgcccgcag cgtgtcgaag gcctgttgtt cgctcaaatg gcgcaggaag
    32701 ctcatcagca ggtagctggt gaaaccgtgg tggtcgcggg cttcgcgcag tgcgcgtgcc
    32761 atccccgcga agatggtttc catggcgacg ccgcgcgccg tgtgcgtctg ggggtcgaac
    32821 atgatttccg cgtggacgat gccgtcggcc cgggcgcgcc gcacgtaggc catggtcagg
    32881 tcgtaaaaat cctgttcagt gatgagcacg ctggcgccgg cgtagtacag atccagaaag
    32941 gactgcagat cggtgaacgc gtaggccgcg cgcagcgatt caacggaatc gtagggtagg
    33001 gtcaggtgat tccgggcggc gaggccgaag atgagttcgg gttcgagcgt gccctcgata
    33061 tgcagatgca actcagcctt gggcaggcga cgaatgaact cggccagcgc gtgggcggga
    33121 caggcggggg tgggcacgat gcgggctccc ggtcgggtga aaacgtcatt ataagaagcg
    33181 gacatttccc gatcgcctcc cgccatcaag gtgtgcgctt tcccttgagg cttttcttgg
    33241 tgcgctccag gacccggctg acatccttgc ccagcgccat ggccacgctc agtgccagca
    33301 ggtccacgat caccagatgg accaggcgcg acagcatcgg gctgtagagc tccgtatctt
    33361 cctgtgtatc ggcgcgcaac agcacgtcgg cggactgggc caatggcgtg ttcgaggccg
    33421 tgattgcgat gaccgggcag tcgttgcgcc tggcggtggc gacagcgtcg agcaggtcct
    33481 tgctgcgccc cgagtgggaa atagcgacca gcacgtcacg cggccccagc atggaggcgg
    33541 ccatcatctg aatgtgcggg tccgaatatg ccaccgtgca gaggtcgtag cggaagaatt
    33601 tgtgttgcgc gtcggcggcc acgatgccgg aattccccag gccatgaaat tcgatgcgcc
    33661 gcgcacgggc caacaatgcc acagcctcgt ccacccgttc cgggtccaga tcgttgcgcg
    33721 cgcgcagcaa ggccgacacg gcattgtcga acaccttgcc ggccagtgcg gcggtggatt
    33781 cccccgaact gagcgaggaa tgcacataag ggattcccga catcaggctg gccgtgagcc
    33841 gaaccttgaa atcgggccag ccccggcacc ccagcgaacg gcagaagcga atgaccgtgg
    33901 gttcgctgac acccgcccgc cgggcgatgt cgcccacggc ggatttggcc gcatccccgg
    33961 gatgctgaag gacgaactcg gccgctttgc gttcggcggc gctgaggctg gggatttgct
    34021 gtcgaagtcg atcgagcatg gtcaggaaag cgtgttgagt ccggcgtcag gcatgccagt
    34081 acacatcgat gggggtggag gtctggttca gcacgaagct gacgggatag tccacggtcg
    34141 tggcgctggc tgcctgctca tagatttgtc gtttgttctg acccgcgatg gccaggatga
    34201 gctgactggc gccgagcagc gcccgaagtg tcatgctgat gcgttcatgc gacgccgccg
    34261 gcggtgtgac gtgaaggtaa cggcctgtct gcgcggggtc caggccctgg gcaagttgtg
    34321 cggcgcgggg aaacaacgac gctgtatgcc cgtcgtcacc catgcccaga acgacgaggt
    34381 ccagatgctc ggtttgacgg ttggcctgct ccaggctgcg gtcaagattc gcgggatcgc
    34441 agaccagtcc cgtgaatcgc gcgctgcggg cctcgtttgt aagcaagtat gtgcgcacca
    34501 ggtgctcgtt gctatcggcg ttgtccgggg cgacgaaacg ttcgtccacc aatcgcacca
    34561 gcaccctggt ccagtcgaca tcggaccgtt ggctcaaggc ctggaacaat gcgatgggcg
    34621 agtgcccgcc ggaaacggcc aggcatgccc ggccgttgat cgtgatggcg gcccgcagca
    34681 gcgccgcgat gcgcgcggac agggcctcga cgtatccctc gttggtatcg aacgtgcgta
    34741 gcatggtcag gtctcctcgt gccaggatac gcctgcgcgg gagagcaggc tgctggaggc
    34801 cgccggcccc cagctgccgg cggtgtaggt gcggggggcg atggagtggt gctcccatgc
    34861 ctcgatgatg ggttcgaccc aggtccaggc ctggacgagt tcgtcacgtc gcatgaaaag
    34921 ggcgagcttg cctcgtatga catccagcag cagccgttca taggcgtccg cccagcgagc
    34981 cttcgacatg gtgtggaagt caaggttcag ataggtggac tgcagatgca tggcgtcccc
    35041 cggctccttg gcgaggaagt acagctgaat gctctccttt ggctgcaggc ggatcaccag
    35101 gcgattggcg gaatgcagat tcaggggcat ggggaagacg gcgtgcggca cgttgcggaa
    35161 gttgatgacg atctccgcca cgcgctcttg caaccgtttg cccgtgcgca ggaagaatgg
    35221 cacgcccgcc catcgccagt tggcgatttc agcctgcagg gccacgaatg tttcggtttt
    35281 gctgtcgggc gcgatattgt gctcgtccag gtaacccacg acaggctggc cgttgatggc
    35341 gccgctgcgg tattggccgc gcacggtacg ggccacgaca tcttctgtgg tgaagggttt
    35401 gagcgatttc aggaccttga gtttttcgtc gcgcaccgcg tcttccgcca gacttgccgg
    35461 tggctccatg gcgacgatgc acagcaattg cagtaagtgg ttctgcagca tgtcgcgcag
    35521 agcgcctgtg cggtcataga aatcgccgcg ttcctccaca cccagttgtt cggcgatggt
    35581 gatctggacg ttgtcgatcc attcccgccg ccagagtggt tcgaacaagg cattgccgaa
    35641 acgtatcgcc atgaggttct gcacagattc cttgcccagg tagtggtcga tacggtagat
    35701 ctggttttct gcgaaatggc gtccgacttc gtcattgatg gcattggacg acgcgagatc
    35761 gtgacccagc ggtttttcca gcacgacacg cacgtttgga tgattcaagc ctacacttgc
    35821 caggtgtgtg cagatcgtgg tgaagaggtc cggcgccgtg gccaggtagc acaccgtcac
    35881 ccggttgcca gggccgccca gcgcacccac cagttgtccg tagtcggctg cgttgccggc
    35941 gtccagcctg tggaaatgaa tgcgtttgag gaaatcggcc cataccaacg ggtcgaagct
    36001 atcgcccagg ccgggtttga cgtgggtctc caccaacgcc aggtagcccg cctgctcgat
    36061 gtcgcggcgc cccagcgcga agatgcgccc atcgggatgc agtgtgcccg ctttgtgtgc
    36121 ctggtatagc gcgggcagca gcttgcgcag ggtgagatcc cccgtgccgc cgaagaaaat
    36181 gagatcgaaa ctcggagtat tggccatggc cggattccct gtttggcggg gcgccgtcgg
    36241 cccgattcgg cggcggcggt tcatgcgtcg tgcatgactg taagcaggac gcttggaaat
    36301 atgtaattta gctacatggt gattgatttg taaagcccgc gtttcgcgga tccatagggt
    36361 aagtccctga tcgggtccca tggttcccat gaaatgatga aaatcacgtg atcgatttca
    36421 acttcatggc caatcgcgcg gtgcatgttt tgcgggatcc aaagccaggc ccgtcgggcc
    36481 gtgtctacat ttacaatacg aatgggccgt tctgtcggcc ggtccgcctc ccagtgtcct
    36541 catctcctca tgataaaaaa ttccagtcgc gcaacgatcg ctgatgtggc acgccacgcc
    36601 gaggtatcca aggcaacggt ctcacggttc ctgaatcatc gtgaaaccct gctctcgccg
    36661 gaaatggcca cgcgcgtcga ggccgccatc cgtgaattgg gctacagtcc cagccccatg
    36721 gcccaggcgc tcaagcgtgg ccgttcccgt ctgattgggt tggtggtggc cgacatcacc
    36781 aacccgtatt cggtggccgt gttgcgtggc gccgaagagg catgtcgaga ggccggttac
    36841 ctggtgatgc tgttcaacct gggcaacgag agcgcgcgcg aaagcgcggg catacgcgca
    36901 ctgtcgtcct atcaggtcga gggtttcatt ctcaacacca tggggcacga tgcaggcgcg
    36961 gtggtcgaaa ccgcccggca gggcaagcca gtggttctgg tcgaccgcct gcaccacggt
    37021 ctggatgttg atttcgtctc gctggacaac gatcaggctg tccatctgtg cgcgcggcac
    37081 ttgtgcgagg gtgggtaccg cgaattgctg ttggtcagcg aaccgctggg cacagtcagc
    37141 tcacgcatgc agcgtctggc ggcatttcag tcgtttgtcc aggaccccgc caatgccgtg
    37201 tcggggcggg gggtggagtg tggtgaggat gataatcggc tggtggaagc cttgcgagcg
    37261 ttgcgcggcc gagccggaac cggcatcccg gccgtgatca gcagcaacgc ggtggtgacc
    37321 ttgcgcgtcg tggccgcggt ggccagcctg ggctggaccc tggggcgcga tatcgggctg
    37381 gtcggtatcg atgagaccga atgggcgccg tacgtggggc ccggtatcag caccgtgtct
    37441 caacccactg gcgagctcgg acgcttggcc actcattgtc tgctcgagcg cctggagggg
    37501 cttgaaacac ccgcaagaac cattctgctt ccaggctcgc tcgtcgtgcg cggctcctca
    37561 tcgggtcaat gacgcgcctg gaggattcag attggttagt gccgtgtctc acaagttcgt
    37621 ttcattagga agtcgagaat cgctgccatg cggcgttgca aatcctcgcg atagcaaagg
    37681 ccagtgctgc ggtttgcgcc ttgcctgatg acgatttcga cttctttttc attcaacttg
    37741 tgagacgcgg aattagggta aatacgtatt tttgaagttt ctttttggcc ggtaaactgt
    37801 cagaaaaagg catgaaaccg gtttcatata tttgtcgcac cggcagctgg gcatctcctg
    37861 gcagcttgtc ggcggggatt gaacaacgtc gtttttcgcg catgcgggtt gctgaacctc
    37921 atgccggaga cgcacggaac aaggcaagta tccaacgtgc attattcatt tcaattcggt
    37981 ccggtcttcg aggcctggcc ttcgctgctg caaggcacct ggaacacgat cgttctgtcg
    38041 ttcctggcca tgctcttcgg gctcatggtg gccattgtct gtgcctgggg taaaacgtcg
    38101 ggccccaggg tgctgcgctg ggtcatcaac gcctacatcg aactcatccg caacacacca
    38161 ttcctggttc agttgttctt tttctatttc gggctgccag cggttggcgt gcggtggtcg
    38221 gcctacacgg cggccctggt ggccatgatc gtgaatcttg gcgcctatgc cgccgagatc
    38281 gtgcgggctg gtattgaatc cattccaaaa gggcagatcg aagctggcct ggcgctgaat
    38341 ctgtcccgcg tcgaaatctt ccgtttcatc attctcaagc cggcgctgcg cgccatctac
    38401 ccggcgctga ccagtcagtt catcctgctc atgctcgcgt ccagcgtggt gtcggtgatc
    38461 tcggccgacg acctgacatc catcgcggtg aatctcgtgt cgcagacgtt ccgcagcttt
    38521 gaaatctaca tcgtggtcac ggtgatctat ctgctgctca ccctggcctt ctcattcctg
    38581 ttccgccgcc tcttcctgct gggcctgaat tacccggacc gtcgataatg cgcacttttg
    38641 gctataacga atttctctac gtcctggaag cggcccgctg gactattctg ctgtctcttg
    38701 tcgcgttcgt gggcggggtg gtggtgggct tgatcattgc cttggcccgc acgtccgata
    38761 gcaagctggc gcgcacgctg gccatcggct acatccagtt gttccgcggc acgccgttgc
    38821 tgctgcagtt gttcctgatc tttttcgggg ccccggtcct gggttgggag atcaacccct
    38881 ggtgggcggc gggtatcgcg ctcaccctca acagcagcgc gttcctgggc gagatctggc
    38941 gcggctgcat cgaggccatc ccccaggggc agtgggaagc cgccgatgca ttgggcttgt
    39001 cgttcatctg gaagatgcgc gatgtggtgc tgccgcaagc gctgaagatc gcggtgccgc
    39061 ccacggtcgg ttatctggtg caagtggtga aagggacgtc gctcgcggcc atcatcggtt
    39121 tcaccgaact cacgcgggcg gggcagatca tgaacaacgt caccttccag cctctgtggg
    39181 tgttttccgt cgtggcactg atttatttcg tgttgtgctg gccgctgtcg ctgctggcgg
    39241 cttatctgga acgacgtctt tccgccggga tggcccggta gttccgagtg ttcaatcgtt
    39301 gtctggctat tggagaactt tcatgtattt ttccagaaaa atccgtactt tcgtgggggc
    39361 ggccgcgctg gtcaccctgg gctccgtggc gttgggcacc gtgtcggcca atgccgccga
    39421 aaccatcgat caggtcaagc agaaggggga gctcacggtc ggcatgctgg tcgacttccc
    39481 tccgtacggc attctcaaca gcgacaacaa gcctgacggc tacgacgccg acgtggcccg
    39541 cctgatggcc aaggattggg gcgtcaagct caagatcgtc ccggtcaagg ggcccaaccg
    39601 gattccgtat ttgctgacgg gcaaggtgga tctgctcgtg gcctcactgg ccatcacacc
    39661 ggaacgcgcc aagcaggtgc agttctcgca gccatactcg gcggcgcaga tcgtcctgtt
    39721 cgcgcgcaag ggcgacaaga tcacgtcgcc caaggacctg tcgggtctgc gtatcggggt
    39781 ggcgcgcgcc agcacacaag acgtcgccgt caccaagatc gctcccaaga acgccaatat
    39841 ccgtcgtttc gatgatgacg cctccgccat gcaggcgctg ctgtccgggc aagtggacgc
    39901 cattggctgc tccaccacgg tcgccgcgca gatcgccaag cgcgatcccg gcaagttcga
    39961 tgccaagttc gtgctgaagc aacaggaaat ggccgttgcc atgcgcaagg gggaacccaa
    40021 gacgctcaag gccgtcaatg ccttcgtcga gaagaacatc aagaatggcg acctgagcaa
    40081 gctctacgaa aagtggctgg gcacgccgtt gcccgacatg aaggccgctt cctgaaaacc
    40141 tggttcgaga gattcgcatg actgatgaac aaggtgttgc ctcggggcgt tccgcgccgc
    40201 gcggcgagac gatcatcgag gcccgcgcgg tcgacaagtg gtatggggaa ttccatgttc
    40261 tgaagagcgt ggacttgaat gtccgggcgg gcgagcgcat tgtcatctgc ggcccgtccg
    40321 gatcggggaa gtccacgctg atccgctgtc tcaacggcct cgagaaggtg cagaagggac
    40381 atgtgatggt ggacggcatc gaactgacca gcggcgcgcg ccaggtcgag gcggtgcgcc
    40441 aggaagtggg catggttttc cagcagttca accttttccc ccacatgacg gtgctcagaa
    40501 actgcatgct ggcgccgatg cgttcgcgtg gcgccggcaa ggcggaggcg gagagcctgg
    40561 ccatgaagta cctgacgcgg gtgcgcatcg ccgatcaggc gcacaagtac cccagccagt
    40621 tgtcaggcgg gcagcagcag cgcgtggcca ttgcccgggc cttgtgcatg acacccaaga
    40681 tcatgttgtt cgacgaaccc acgtcggcgc tcgatccgga aatggtcaag gaagtgctcg
    40741 acaccatgat cggcctggcc gacgacggca tgaccatgct gtgcgtgaca cacgaaatgg
    40801 ggtttgcgcg ccgcgtcgcc gaccgggtca ttttcatggc cgatggcgaa atcattgaac
    40861 aggcgccacc agaccagttc tttgataatc ctcagcatca gaaggcacgc agcttcctgg
    40921 ggcagatcct gaattcctga taccggacac acaccttcca tgagtctcgc aaggcaaatc
    40981 atcgtatccc tgtcatcatt cggggcttcc aacgtgcgcc tgcacggcca acggcgttac
    41041 gtcgagttgt gcgcggaggc gggcgcggat ggcgtggaaa tcaggggtga attgctgaat
    41101 gatccggcgc gtgagctgcc cggcctggcc tcctgcctgg acggagtggg cctgcgcgcg
    41161 gtgttttcca gccccgcgat gctctgggat cggacggggg ccctggatcg cgacgcactc
    41221 gaactaggtc tggccgccgc cgccacgctg cgcgccccgg tgttgaagat gtccataggc
    41281 gggtacggcc aggcttcgcg gacatcgctg gtcgaattgc aaggcgcgtt gtccgacggg
    41341 aaggtggagt tgttgatcga gaacgatcaa acgccgcgtg ccgggtcggt gtcgtcgctg
    41401 cggcgttttt tcgaggccgc cgacgagacg ggcatccacc tgggcatgac gttcgacatg
    41461 ggcaactggc attgggtggg ggaatcgtca ctgcaggcgg ccggcatttt cgcgccacgt
    41521 gtgcggtatg tgcattgcaa gggcgttcag cgccagccgg gccgttgggt ggcggtggcg
    41581 cttcaggagt cgtcggcgcc gtggcgcgcg gtgctgcggg ccatgccgga cctggcgccg
    41641 cgtgcgatcg aatatccact ggtcggggag gacgttgagg cggtgacccg cggagccatc
    41701 caggatctgc gcacccttgg agatgattga tggacccgct tctggatgtc gtcacgtttg
    41761 gcgaggccat gatgctgctc gtggccgatc gtcccgggcc actcgagtcg gtgccggcgt
    41821 tctacaagcg gactgcgggg gccgagacca atgtggctgt cggcctggcg cgtctcgggt
    41881 tgcgcgtggg ctgggtcagc cgtctggggg ttgattccat ggggcgctat ctgctggccg
    41941 cgatgcgggc ggaaggcatc gattgctctc atgtcatctg tgatccaacg cagcgtaccg
    42001 gcatgcagtt caaagggcag gtcgtggacg gcagtgaccc tccggttgaa tatcatcgca
    42061 aaggctccgc ggccagccac atgcagtcgg acgacattga cgtttcgtgg ctttgcgcgg
    42121 cgcgccatct gcattcgacg ggtgtgttcg cggccatctc ggaaaattgc cttgccacga
    42181 ccaccagaac catggcgctc atgcgcgcgg cgggccgcac gatttcattc gaccccaacc
    42241 tgcgtcccac gctgtggtcg accgaggaca gaatgcgcga gtgcgtcaat cggttggcgt
    42301 tcgccgccga ctgggtgttc cccggctttg cggaagggct tctgctcacc gggcatgaca
    42361 cgcccgaaca aatcgcggcg ttctaccgtg gccatggcgc acgtctggtc gtggtgaagc
    42421 ttggtccgga cggtgcttat ttcgatggcg agcaggggca aggctatgtg gtgggctgtc
    42481 cggtcgccaa ggtggtcgat accgtgggcg cgggagacgg cttcgcggtc ggcgtgatca
    42541 gcgcgatgct ggaggggcgc acggtgcgtc aggctgtcga gcgtggggct tggatagggg
    42601 cccgcgccgt ccaggtgctg ggtgatacag agggtctgcc gacacgcgcc gaactcgaac
    42661 tggcggagtc gtgacatgaa cagaaaacag gtgctggtct tcaaggcgct gcccgccgat
    42721 caactggcgc ggctgcgaca cgagtgcgac gtggtcgtgg gcgacccgcg cgtaccgcaa
    42781 cagaaggcgg cgtttcaaca agccttgtcc accgtcgatg gcatgatcgg cgcgagccac
    42841 cggttcgatg acgcactact cgacgcggca ccgaacctca aggtgatatc cagtgtgtcg
    42901 gtgggtgtcg acaactatcc gctggcgtcg ctcaagtcgc gtgggatcat gttgtgccac
    42961 acccccgggg tgttgaccga aaccgtggcc gatctgtatt tttccatgat tctcgcaacc
    43021 agtcgccgta tcgtcgaact ggcgaacctt gtgcgcgaag gacgttggac gggcagtatc
    43081 caaccggata tgttcggctg ggacgtgcac ggcaagacgc tgggcatcct gggatatggc
    43141 cgcatcggtc aggccatcgc gcgccgcgcc gcgcgtggct ttgacatgca ggtgctgtat
    43201 cacacgcgca cgcagccgtc cgcaggcgat gccgacaacc gcatcatgcc cgcgtcgttc
    43261 aaggaggttc tgtccgaatc ggattttgtg gtggtggtcc tgcccctcac cgggcagacg
    43321 ctgggcctga tcggttccgc tgagttcgcc atgatgaagc caggcgcgat tttcatcaat
    43381 ggcgcgcgcg gcgccatcgt tcaggaaacc gcgctgctcg acgcactgaa ccgtggcacc
    43441 ttgcgtgccg cgggtctgga tgttttcatg acggaaccct tgcctgcgga ttctccgttg
    43501 cgcacgcatc ccaaggtggt tccgctcccg cacgcgggct cggccacgca tgaaacccgc
    43561 cacgccatgg cggaaatggc caccacgaac cttctgctgg ccctcaaggg gcgtccaccg
    43621 ctggcggtgt tcgacgcgta gcggtgttgg ttgctcatgg ctccatggat gggtttgtga
    43681 gctttggcgc tgttggtatt ttaaggcgct gcccctcggt ggatccgtcg cacgccttac
    43741 accggcccct ccccaaaccc gaggggcagc ttgtggaggt ggtatgtgaa aacagatgaa
    43801 ggcgattcga ctcaggcgtg tgaaccagtc agggcgcagg cttccttggc cagttgcgtg
    43861 atggcatccc actgtttgtt ggtgaccacg gagtccgggg tcagccacga accgcccacg
    43921 cactgcacat tgggaagcgc cagatagctt cgagcgttgc gtggattgat gccccccgtg
    43981 gggcagaaca ccacgtcggg catggggccg tagatggact tcagcagcgc cacgcctccc
    44041 accgcttcgg cggggaacag tttttgcacc atgtagccct gatccgcggc gaacatggat
    44101 tccgatggtg tggcgattcc cggcaagaac ggaatgcctg atgcacgcgc cgcggcggac
    44161 agttcacgag tcaaccccgg gctgactgcg aatcgggccc cggcgtcgga ggcggcctct
    44221 agttgtgcgg cggtgcgcac ggtgccgacg ccgacaatgg catcgggaac ttcctggctg
    44281 atgagccgga tggcatcgat cgcgacgcag gagcgcaagg tgatttcaag gcagcgaaca
    44341 ccgcccgcga ccaaggcctt ggccagatcg acggccaggt cgacgtcctt gatgacgatg
    44401 acggggatta ccgggctttg ctgaaggagt tcgagtgcgt tcatgacggg aagtggctgt
    44461 tcttggatgt tggtgtttga ttcattttgg cgctgcatgt ggcgtgggtc acggagagtt
    44521 gcactttgtt tacatcgcgt tcgcgcttgt cggtgaatcc ggactgccga acagcgtagc
    44581 cccttcttcg gccgacccga cactctgtcg ggccgaagtg aacagttcgc ggccaaggcc
    44641 gacctgacgg ccatgaaggt cggctgtgac cggcttgcgc cctggccagt cgagtccttc
    44701 ttccaccgag agttcaccgg tcgtggcgtc gagccggatc aggtcgccgt cgcgcacctt
    44761 ggcaagcggc ccgcccgcca gcgcttcagg gcacacatgg atggccgccg ggatcttgcc
    44821 ggatgcgccg gacatgcgcc catcggtgac gagggcgaca tgcaggccct gatcctgcag
    44881 cgacgccagg accggggtca gtttgtgcag ttcgggcatg ccgttcgcgg ctggcccctg
    44941 gaagcggatg accgccacga aatcgcggtt cagcaagccc tgccggtaag cggtcagcaa
    45001 atcgtcctga tcgtggaaaa ccagcgccgg ggcctgtacg acccgatgtt caggcgcgac
    45061 agccgaggat ttgatcacgg cccggcccag gttcccgtgc agaaccttca gaccgccatc
    45121 ggggctgaag ggtttccgga cggagcggat aacgctgtca tcccccgtga cgcgcacgcc
    45181 tgcgcgccag gccagacggc catcgccgtc caggaatggc tcctgggtat agcgacgcaa
    45241 gccaggaccc gcacaagtgc ggacatcgtc gtgcaacaac ccggcgtcca gcagttcagc
    45301 gatgaccaac cccacgcctc cggcggcgtg gaagtgattc acgtcggccg ggccattggg
    45361 gtagacgcgt gccaacagcg gcaccaccgc cgacagctcg gcgaaatcat cccaattgat
    45421 aatgaccccc gccgcccgtg cgatggcaac caggtgcaga gtgtggttgg tggagccgcc
    45481 cgtggccagc aggccgacga tggcgttgac gatggtggct tcggtcacca cttcggcaac
    45541 gggcgtgaac gtgtcacctt gctgactgat ggccaccgcg cgttgcgcgg ccgcgcgcgt
    45601 gagcgcgtcg cgcagcgccg tgccgggtgg cacgaacgcg ctgccaggca gatgcaggcc
    45661 catgatctcc atgagcatct ggttcgagtt ggccgtgccg tagaacgtgc aagtacccgg
    45721 ggagtgatac gagcgcgatt cggattccag cagttcggcc cgcccgactt tgccttgggc
    45781 gaagagctgc cgtgtcttcg ccttgtccgc attggcgatg ccactgggca tggggccggc
    45841 aggaacgaag atggcgggaa gatgcccgaa agacaaggca ccgatcagca ggccaggcac
    45901 gatcttgtcg cacacgccca ggtagaccgc cgcgtcgaac acctgatgac tgagggaaat
    45961 ggcggtggac atggcgatga catcgcgcga gaacagactc agttccatgc cgtcctggcc
    46021 ctgggtgatg ccgtcgcaca tggccgggac gccgcccgcg aattgtgccg tggcgccgac
    46081 ggcgaacgcg gcttgcttga tgacggtggg gtaggcaccc agcggctggt gtgccgacag
    46141 catatcgttg taggacgaca cgatggccag gttgggtgtg tcgccgtggt ggagccggcc
    46201 tttttcggtc gcgggcatcg ccgccgccgc gtgggcgagg ttcgtgcaac ccaacgcgct
    46261 gcgccgggtc ttgccgccat aggcggcacg agtgcgttcg aggtaatggc gccgcgtgga
    46321 ttggctgcga tcacggatgc gctgtgtgac gttgagaaca gcggggttca tggtcatgga
    46381 atttctcgga aggatgcgta cgagataaaa tgtagtttta ctacatattt tagataaaaa
    46441 gaacccctgg caaagaaaat tattgatttt tttgtaaatg gagtaagagt tttaccgaga
    46501 gaactaggag ttttattgaa ccgcgctatg ccggctttta ttcatgtgac aggaaggtcg
    46561 attttcacaa atttgtaatt ttgctacaaa tatggaatgt gacaacttat cttttatttg
    46621 tggcttgtga tgtgcgactg gggtccggga ggcgggtcag gcgtgtcgat aggcggcaat
    46681 cgccaccatg gcccaggcga tgatgaaacc cagcccgccc aggggcgtga tggcaccgag
    46741 ccaccgtgtg cccgtcaggg ccagcaggta caggctgccg ctgaacagca cgatgccgat
    46801 gagcatgacg atgctggcgc ttgagagcag cggggatccg aagcgtgatc ccaggacggc
    46861 gatggccagc agacccaggc cgtgaaacat ctggtaggtc acggctgtat gccacacggc
    46921 cagcatgtcg gggctgagta cacgcttgag cccgtgcgca ccaaacgcac cggcgccaac
    46981 gcccacgagt agcacaagag cggcaacgat tatcagatgg cggtcggtca tgggggcgtc
    47041 cgtatgggtt ggccccgccg gccaatggcg gagcaagggg ggctgtgcca aggagagcgt
    47101 tttccctggc gtcgcgcagc cagatcgtcc atccccggtt tccggggccg atgctatcat
    47161 gaatttccct catttagcga gcgtccccaa tggcagagtt tgagcagcct tcagcggtac
    47221 cggaaaaggc cgtgatcgcg tccgtgcttt atcgcactgg ccaaccaccg gtcgatgtgc
    47281 cactggagca agtgcacagg ccgtctgatg aaccgaggca gctattgtgg gtggggctca
    47341 gggaacccga actcgaaaca cttcgcctga tgggcgagcg gttggggctg ggcgcgcggg
    47401 ccatagaaga aatcaccgaa cgtcatgtgc gtccgaagat tctcgaattc gaacacatga
    47461 cgctggtggt ggcggtcacc atagaggtcg agaacctgcg cccagtgttt ggcgaaaccc
    47521 aattgctgat cggtcgcaat ttcctgataa cggtgcggcg cggggcggtg gccgcgcaca
    47581 acagcctgcg ccagtacctg gaaggcgtgc cggaattgct cggccggggc agcgactacg
    47641 tggcgtccat gctgctcgac ctgctggccg atcgctactt ggccgcgctt accaagttcg
    47701 aagccacggt ggaggcgatc gagcagcagt tcatgttgcg gggattcggc gatgtggatg
    47761 tgcgcaagct gtatcgcctg cgccgggacc tgctgcgcat gcacacggcc atttttccca
    47821 tgacagagat ctgtcgacgc cttgcgcgcg tcgacacggg gcacatcgat gccaattgtc
    47881 gtggctattt cggcgaggtg gccgatcgtg tgcaacgcat tgacgaactc atcaacagtc
    47941 tgcgcgaggc cctggcgttc gccttcgagg cgggcagcat gctggggcag tcccagcaaa
    48001 ccgacatcac caagaagctt gccgcgtggg cggccatcct ggccgttccc acggctgtcg
    48061 cgggtatcta cggcatgaat ttcgtatcca tgccggaact taaatggcat tacggttatc
    48121 cgttgatgat ggggtgtacg gcggtggcct gcgccatact gtattggcga ttccgaaagg
    48181 cgcgctggct gtaggcggaa tggaggtgct gggatggaat ggtcaacgca tcaggtcacc
    48241 aatcaagtac ccgatctggt cggctacaac ctgttcgacg ccgatgtcgc cctgaacgac
    48301 gcggtgcggc gcgaaggagc tgccgctttc gaacaaaacc tgcgggatta tggctcccgg
    48361 ttgggaggcg ttgaggtcgc cgagttggct cttcgcgcga accaggatcg tcctaagctc
    48421 gaaacatttg accgattggg gcatcggatc gatcaggtgc gcttcgaccc cgcctggcat
    48481 cagctcatgg gcatggcgtt cgagcaaggc atgcacgtgg gtgtggtgac tggctcagag
    48541 ggtttccagg ccggctggcc cgcggcccgc gccgctttgt acttgctgca tgggcagatt
    48601 gaagcgggca ccttgtgccc cctcaccatg acggcggcgg ccgcgcccgc gttgcgcgac
    48661 gaaccctggt tccatgatct cgcgccacat ctgtactccc ggcggtacga cccccgcgac
    48721 atgcctgtgc cggaaaaggc gggggtgacg atagggatgg gcatgaccga aaagcagggc
    48781 gggtcggatc tgcgcgccaa tgccacgcag gcttttccgc tggaacaggg ggcggaacgc
    48841 ggggcggcgt tccgcctggt tggtcacaag tggttttttt cctcgcccac gtccgatgcc
    48901 catctggttc tggcgcactg cggcggtgct ttgtcgtgtt tcttcgtgcc ccgttggaca
    48961 cctgacggta cccgcaacgc ggtgcgcatc cagcggctga aaaacaagat gggcaacgcg
    49021 tccaatgcca gtgccgaagt cgagttccag gatgcgttcg gcatcctggt tggcgatccc
    49081 ggccgtggta ttcccattct ggctgaaatg gccacccgca cccggctgga ttgcgtgctg
    49141 ggcagcactg ctctgttgcg ccaggcactg gtgcaggccc tgcatcatgc gcgaacccga
    49201 caggctttcg gcaggaagct ggccgaccac ggcctcatgc gccaggtgct ggctgacctg
    49261 gcgttggaaa gcgaggcggc cacggtgttg gccatgcgtc ttgccggggc gctcgaaaaa
    49321 agaggctcct ctgccgagga acgtgtctgg gtgcgcatcc tgacgccggc ggcgaagttc
    49381 tggatctgca aacgcgccat ccaggcggtg gccgaatgca tggaggtctg gggcgggaac
    49441 ggctacatcg aagacggccc catgcctcgc ctttaccgtg aaacaccggt caactccatt
    49501 tgggagggct cgggcaacgt catgtgtctg gatgtgttgc gcgccttgaa tcatgatgcg
    49561 gacgccggcc gcgcttttgc cgatacgctg gttcgcgatg ccagcgtcga tcggcctctg
    49621 cgcgcccggg cggagcgcct ggtgaaacgc ctgcgcacgc ccggcgccaa tccgcagccc
    49681 tgggcgcgtc acatctcgcg tgaaatcgtg ctgctgtcgc aggcggtgtt gctgcgcaag
    49741 cacgctcccg cgccggtggc cgatgctttc atgcagacaa gaatccactc ggaggctgac
    49801 ggcgttttcg gcagcggcat gccggtgggt gctgccgaaa cccttttggc ccggtcctgg
    49861 ccggtgtaga tgaatcgctt cctgaatcct ctcgttcgcc aactgtcccg tgcgggcgca
    49921 ttgcatcggc gacccgccgc ctgggcggtg ctggctggag tgctcgtcgt ggccgtgggg
    49981 gtggcgtcaa aggctggttt cgtggtctcc gggcaacgcg aggccggagc atccgacaag
    50041 gttgcgtaca ccctcgcggg gcgggtggtg cacgtggcgg atggcgatac gttcacattg
    50101 ctggttgatg gccgccgtga acgcatacgc atggccagca tcgatgcccc tgaaaccagg
    50161 aagaatcgcg agcgtcccgg ccagcctatg gcgcaggcgg cgaaacaggc attggccggc
    50221 ttggtcgcgg gcaagaccct tgcgctttcg tgcttcgagc atgatcggta cggacgcaat
    50281 gtgtgcgacg tgccgctgcc tgacggctcc accgccaacc gtgagctcgt ggcggagggg
    50341 cttgcctggg ccaacggcga aggacaaggc cggtacatgc gcgaccggcg ccttccagga
    50401 ttggaagcgg ccgcgcgcga ggcgcgcaaa ggtatctgga gcgaaccgtc gcccgtgccg
    50461 ccctgggtat ggcgttacca gtgctggaaa catgggcagt gttgatgtgt ggtttcgcga
    50521 tgacaatggc tattgccacg tcttgcggcg tccctattga ttttttttca tttggcccca
    50581 tcatgagggt atgtcactga aagtattcga cctccaatgc gattccggcc acgttttcga
    50641 gggctggttc ggctcggccg atagctacga atcgcaacgt gaaaatgggc tgctgacctg
    50701 tccggtctgc aacagccata acgttgtgcg caaactgtcc gcgtcgcgcg tcaacaccgg
    50761 cgccggccgg gatccggccg ctggcgagag ccgccccgcg caagcccagg cagttgccgc
    50821 cccggcgggg cggcaactgg cccagcttca ggccgaaatg ctgcgccaga ttcgtgaaat
    50881 cgtgcgcggc gccgaaaacg tggggccgcg cttcgccgaa gaagcgcggc gcatgcacga
    50941 gggcgaggcc cacgaacgcg ccattcgtgg cacggcaaca gaagaagagc gccaggaatt
    51001 ggcaagcgag ggaatcgcgg tcatgccggt tcccgacttt ctggatgacg accgcctgca
    51061 ataaggcgtg tcccgcacgg cccgtggcgc cgttccgccc atggcgggcg gcatcggggg
    51121 aatgaaaggc ccggcatagc cgggcctttc gtcttacatc acgcggctgc ggtattcgcc
    51181 ggtgcgtgtg tcgatttcga tcttgtcgcc gatttcgcag aacagcggga cgttgatgtc
    51241 catgccggtg ttcgtcttgg cgggcttgag caccttgccc gacgtgtcgc cgcgcaccgc
    51301 gggttcggtg taggtgattt cgcgcacgac gatggtgggc aggtcgaccg aaatggcccg
    51361 gccgtcatgg aagacgactt ccaccgccat gccttcttcg aggtagctca gggcgtcgcc
    51421 catgctgtcg gcttcgactt catgctggtt gtattccgcg tccatgaaga cgtacatggg
    51481 gtcggcgaag tacgaatacg tgcattcctt cttctccaga accacgatgt cgaatttttc
    51541 atcggccttg acgacggatt cactgcccga cccggtcagc aggttcttga atttcagctt
    51601 cacgacggcc gcattgcgcc cggacttgtt gtattcggcc ttctggacga caagggcgtc
    51661 gctgccgacg gtcaccacgt tgccaacgcg cagttcttgt gcggatttca tcaatttgaa
    51721 gtgctccatg caatggcttc gccagaaagg gagatgcgaa gggggcgtgg cgaaagcgta
    51781 ataaaagtgc aaaacccgga attttagcgc gtttgccggt attttgcgca gaaggcaaga
    51841 agcgcttgcg ccaacccgga actttgggcc agagagccgg cccatcggcc gctggcagcc
    51901 tgccatcggg cccacgtggc gggctgcaac gtctgttcca ggctgtcgcg gaatgccggc
    51961 gtgtccccgg cgttccaggc gcgcaggccg gcatctgctt ccgcggggta gctcgccagg
    52021 cgcagccagg catcgagctt gtccatgtgg gtgttttcgt cctgcgggta gatgtgccag
    52081 atcagcggct tgcccgccca gatggcgcgc acgagcgaat cttcgccccg caccaggttc
    52141 aggtcgctgc tccacaacag
//
LOCUS       DASTNK010000145         3112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_309627, whole genome shotgun sequence.
ACCESSION   DASTNK010000145 DASTNK010000000
VERSION     DASTNK010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309627"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1136
                     /locus_tag="VFR20_11090"
     CDS             168..1136
                     /locus_tag="VFR20_11090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEU0231108.1"
                     /translation="MFNTLRAVAAAGIAVFCTFGLAATSHAEQRIAYKSAKTGTSYYQ
                     MGVELANAIKKGTKGGTILTIEESQGSVQNVMEASVRAGNYVFTSPPGLVESAMAGKG
                     PFKNRPKEAFAKIRALFPIPSLTMHFVMRGDKHVDHFSDLAGKTILLGKGSFGAREGA
                     KYLKLFGLDGKVRIASAELGNAADALKNGQIDGFVTAGSFPAPNVVQAAASTPVSLVS
                     MTDAEIAKTGRTKQIIPAGTYAGVNQDVDTTSLPVEAFTTTAMDDNTAYMLTKTFWQQ
                     KALLSKDAPWWKAVTPEMLMGTGVKLHPGAIRYYEEVGIKVPANMR"
     gene            1308..>3112
                     /locus_tag="VFR20_11095"
     CDS             1308..>3112
                     /locus_tag="VFR20_11095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEU0231109.1"
                     /translation="MASIASDSPHSAATRPPPRVWVFLGLVSIVFHLGLIFSGLVPAL
                     VARPLHMALALPWLFLFAARSRSQRISGVILMLAGEAACLYTAFNADMLSNQYGFLNG
                     NVQIIVALALLAITLEMARRAIGWPLPMVALVCLLYGFFGQYIPGQFGHPTVPFESTL
                     GTLTITEGGVWGPLTGVSVSVVAIFVIMGAVLNVGEAGQGFLNIAIAAAGRLRGGAGK
                     VAVLSSALFGSISGSASANAASIGSVTIPTMVRLGYPKSLAGAVEAVASSGGQIMPPL
                     MGAGAFVMVELTGTPYTQIMAAALLPALLYFLAVWIGIDLFARRYGLKSLDQNDRPHR
                     RDVIITMLFFLVPFAVLLQQMFIAQVTPQYSACMAILVGVLLLFVNIRFEISWRRFAQ
                     RMSDAAILAGKQISMIAAIIICASVVIGVLGMTGLGVKVTSVILAVAQDSLWPALLLT
                     ALACLLLGMEVPTTAAYVICVAIAGPALHSLGINLLLAHLFVFWFALLSTITPPVCGT
                     VFIAAGIVGENWLKVAGSAMRLGLGLYIIPLAMIANPAIIELETHPWLAVFAFARIAV
                     GLWGLSFAFISGQKVWLRLPAGIAGAAICFGPAFM"
BASE COUNT          532 a         1008 c          938 g          634 t
ORIGIN      
        1 cgcgtcgtct tgctgtgtgg acagtcctcg cagaaacggc ccggacaaag catccgccca
       61 tctgccgcgg acctacacaa tatccatggc cgagttgacc cactcggaaa attgcgttta
      121 ttctccatgg gttttgggga aacattctgt catcaagggg acaaagcatg ttcaacacat
      181 tgcgcgcggt cgcggcggcc ggcattgcgg tgttctgtac tttcgggctg gcggcaacga
      241 gccatgccga acaacgtatc gcttacaaat cggccaagac cggcacatct tattatcaga
      301 tgggtgtgga actcgccaac gccatcaaga agggcaccaa gggcggcacc atcctcacca
      361 tcgaagaaag ccagggttcg gtccagaacg tcatggaagc atcggtgcgc gcgggcaact
      421 acgtgttcac gtcgcctccg ggcctggttg aaagcgccat ggctggcaag ggccccttca
      481 agaaccgccc caaggaggcc ttcgcgaaga ttcgcgccct attccccatt ccttcgctga
      541 ccatgcattt tgtcatgcgc ggcgacaagc atgtcgatca cttctccgac ctggcaggca
      601 agaccattct ccttggcaag ggcagcttcg gcgcccggga aggcgccaag tacctcaagc
      661 tgttcgggct cgatggcaag gtcaggatcg cctcggcgga actcggcaat gcggccgacg
      721 cgctcaagaa cgggcagatc gacggtttcg tgacggcggg ctctttcccc gcgcccaacg
      781 tggtccaggc ggcggccagc acgccggttt cgctggtgtc catgacggac gccgagatcg
      841 ccaagacggg ccgcaccaag cagatcatcc cggccggcac ctatgcgggc gtcaaccagg
      901 atgtcgacac gacgtccctg ccggtcgagg ccttcaccac cacggcaatg gacgacaaca
      961 ccgcatacat gctcaccaag actttctggc aacagaaagc cctgctcagc aaggatgcac
     1021 cctggtggaa ggccgtcaca ccagaaatgc tgatgggcac tggcgtcaag ctgcatccgg
     1081 gcgccatccg ctactatgaa gaagtgggca tcaaggtccc ggcaaacatg cgctgatcac
     1141 tcgtcaagaa cgtcgccact gacggcaatg accggtgccc gcgcttccaa atgcgcgggc
     1201 accattcgtt tttggcacca atccgtccac acacatatag accgcccgct tgcatcggca
     1261 tgtccgcacc atacggaaca ggcgagtctc attcacgata catgaccatg gcatccatcg
     1321 cgtccgactc accgcattcc gcggcgaccc gccctcctcc ccgcgtctgg gttttcctgg
     1381 ggctggtttc catcgtattc cacctgggcc tgatcttcag tggtctggtg ccggcgcttg
     1441 ttgcgcgacc gctgcacatg gccctggccc tgccctggct gttcctgttc gccgcgcgtt
     1501 cgcgcagcca gaggatctcg ggcgtcatcc tgatgctggc gggcgaggcg gcgtgcctct
     1561 acaccgcctt caatgccgac atgctgtcga accagtatgg gtttctcaat ggcaacgtgc
     1621 agatcatcgt cgccctggcc ttgctggcga tcacgctgga gatggcgcgc cgcgccattg
     1681 gctggccgct gcccatggtg gccctggtgt gcctgctgta tggctttttc ggccagtata
     1741 ttcccgggca gttcggccat cccaccgtgc cattcgaaag cacgctgggc acactgacga
     1801 tcaccgaggg cggtgtctgg ggcccgctca ccggtgtatc ggtgagtgtg gtggcaattt
     1861 tcgtgatcat gggcgcggtt ctgaatgtcg gggaagcggg ccaggggttc ctcaacatcg
     1921 ccatcgccgc ggccggccgc ctgcgggggg gagcgggcaa ggtcgccgtg ctttcttcgg
     1981 cgctcttcgg atcgatttcc ggttcggctt cggccaacgc cgcctcgatc ggctcggtca
     2041 cgataccgac catggtacgc ctgggctacc ccaaatcgtt ggcgggagcc gtcgaagcag
     2101 tagcatcctc gggcgggcag atcatgccgc cattgatggg ggccggcgcc ttcgtcatgg
     2161 tggaactcac cggcacgccc tacacccaga tcatggcggc ggccctgctg ccggccctgc
     2221 tgtatttcct ggctgtgtgg ataggcatag acctgttcgc ccgccgctac gggttgaagt
     2281 cgctggacca gaacgaccgc ccacatcggc gcgacgtcat catcaccatg ttgttcttcc
     2341 tggtgccatt cgcggtgctg cttcagcaaa tgttcattgc acaagtcaca ccgcagtatt
     2401 cggcctgcat ggccatcctg gtcggcgtgt tgctgctttt cgtgaacatc cgtttcgaga
     2461 tctcgtggcg gcgtttcgcg caacggatgt ccgacgccgc catcctggcc ggcaagcaga
     2521 tctccatgat tgccgcgatc atcatctgcg cgtccgtcgt gatcggcgtg ctgggcatga
     2581 ccggactggg cgtgaaggtt acgtcggtga ttctcgccgt cgcacaagac agcctgtggc
     2641 cggccctgtt gctgaccgcg ctggcctgcc tgctgctggg catggaagtg cccaccaccg
     2701 ccgcctacgt catctgcgtg gccatcgccg ggccggcgct gcacagcctg ggcatcaacc
     2761 tgctgctggc ccacttgttc gtgttctggt tcgcgctgct gtcaaccatc accccaccag
     2821 tctgcggcac cgtcttcatc gcggcaggca tcgtgggcga gaactggctg aaagtcgcgg
     2881 gctcggccat gcgcctgggc ttgggcctgt acatcatccc actggccatg attgccaacc
     2941 ccgccatcat cgaactggaa acccacccct ggctcgcggt gttcgctttc gcgcgcatcg
     3001 ccgttggcct gtgggggctg tcgtttgcgt tcatctcggg gcagaaagtg tggctgcgct
     3061 tgccggcggg tatcgcgggg gccgcgatct gcttcgggcc ggcgtttatg ta
//
LOCUS       DASTNK010000146        27715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_310736, whole genome shotgun sequence.
ACCESSION   DASTNK010000146 DASTNK010000000
VERSION     DASTNK010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 27715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27715
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310736"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFR20_11100"
     CDS             complement(<1..655)
                     /locus_tag="VFR20_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU0231110.1"
                     /translation="MLDPNQLRKDPSTVVERLALRGVQFDTERFNTLEARRKAVQVET
                     ETLQARRNAVSKLIGQLKSKGQDASQELAESQAIPERLKQLAADLAGVQSELNDWLMT
                     LPNLPHASVPAGASSDDNVEVRRWVPPGVEVDGQGCPRAFPFETRDHVALGEPLGLDF
                     EAARKLAGARFMMLRGQMARLHRALAQFMLDLQTGEHGYTDCYTPYIVNGATLFGTGQ
                     "
     gene            complement(854..2242)
                     /locus_tag="VFR20_11105"
     CDS             complement(854..2242)
                     /locus_tag="VFR20_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU0231111.1"
                     /translation="MPESGNLFTSPVAGSHAPLAERMRPKSLDDVVGQTHLLGPGKPL
                     RVAFQSGRPHSMIFWGPPGVGKTTLARLMADGFDAQFLAISAVLGGVKDIREAVVLAQ
                     AAQAQGRRTILFVDEVHRFNKAQQDAFLPYVESGLFTFIGATTENPSFEVNSALLSRA
                     RVYVLEPLSVDDLRGLLDRALEMFDSTPPPGDDPQGTDGPRATGHRPTPRFDDEARAQ
                     LAAWADGDARRLINAVEVVAEAALGAQRELVDAAWLETALSQNLRRFDKGGDAFYDQI
                     SALHKSVRGSDPDAALYWFTRMLDGGADPRYLSRRIIRMAWEDIGLADPRAMQIANEA
                     ASTYERLGSPEGELALAQAVIYLAVAAKSNAGYMAYNQARAFVRQDTSRDVPVHLRNA
                     PTKLMKELGYGRQYRYAHDEPNGYAAGETYLPDGMARPNWYRPVPRGLEGKIAEKLAF
                     LRQLDDEAKKGG"
     gene            complement(2275..2904)
                     /gene="lolA"
                     /locus_tag="VFR20_11110"
     CDS             complement(2275..2904)
                     /gene="lolA"
                     /locus_tag="VFR20_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEU0231112.1"
                     /translation="MQTKHLLAALCLAACPALAWSASARDQLRQFVASVSVATGTFKQ
                     QTLGTPNDPGKAKPPESGRFAFRRPGRFRWEVKKPYEQLIVSDGKTVYQYDPDLAQVI
                     ERDVSKSIGASPAAILFGSGSLDDAFVVTPLPDKDGLQWLRATPRKADAGFAHVDMGF
                     SDNKPVRLELLDSFGQITRIDLSDIQTNPTLPASEFTFALPKGVDVVHM"
     gene            complement(2894..5251)
                     /locus_tag="VFR20_11115"
     CDS             complement(2894..5251)
                     /locus_tag="VFR20_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEU0231113.1"
                     /translation="MARIPATSPRSSRNVRNGPSPLQLRLAGLVREARWFVFAALAAW
                     LALVLATWNPADPAWSHSVLSTSTSNQGGRLGAYVSDLLLFLFGYSAWLWVVLLAQRV
                     VAGFYRLTSLLLPNHKVETLPRVHWEAGLGFLLLFVGSMGIESLQLKQFGASLPAGPG
                     GQLGRLLAHVMSLSLGNTGCTLLLLVFIAVGASLFFGFSWLFVAERVGTALEKSVRRV
                     FELRAARADRKVGEVKSAERNESVVAQQKQLVHEQPVRIEPAIVSVPKSVRVEKEKQK
                     ALFPMPSELGGSGDLPAVSLLDAPDDNIETVSPETIEYTSRLIEKKLSDFGVKVTVVS
                     AQAGPVITRYEIEPATGVKGSQIVNLAKDLARALSLVSIRVVETIPGKNLMGLELPNP
                     KRQMVRLSEIIGSQTYYSGASMLTMALGKDIAGNPVVADLGKMPHLLVAGTTGSGKSV
                     GINAMILSLLYKADATQVRLILIDPKMLEMSIYADIPHLLAPVVTDMRHAANALNWCV
                     GEMEKRYRLMSKLGVRNLAGYNNKIQDAIKREEPIPNPFSLTPDEPEPLAPMPMIVVV
                     IDELADLMMVVGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRIA
                     FQVSSKIDSRTILDQMGAETLLGQGDMLYMPPGTGLPTRVHGAFVHDDEVHRVVDYLK
                     EQGEPNYVDGLLDGALEGETGDGVSAVTGFANAESDPLYDQAVEIVLKNRRASISSVQ
                     RHLRIGYNRAARLLEQMEQAGLVSAMQSNGNREILVPAGNASDAD"
     gene            5296..6255
                     /gene="trxB"
                     /locus_tag="VFR20_11120"
     CDS             5296..6255
                     /gene="trxB"
                     /locus_tag="VFR20_11120"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU0231114.1"
                     /translation="MTTPKHAKVLILGSGPAGYSAAVYAARANLNPVLVTGIEQGGQL
                     MTTTDVDNWPADVEGVQGPDLMQRFQAHAERFKTEMVFDQIVSVDLSRRPFLLIGDSG
                     THYTCDALIIATGASAQYLGLPSEQQFMGRGVSGCATCDGFFYKNQDVIVVGGGNTAV
                     EEALYLSNICRTVTMVHRRDTFRSEPILTDRLTDKARNGNVRLKLFHELDEVLGDDSG
                     VTGVRLRDNRSGQTEDLAVTGAFIAIGHKPNTAIFEGQVDMVNGYITTRSGLNGLATM
                     TSVEGVFAAGDVQDHVYRQAITSAGTGCMAALDAQRWLENAEK"
     gene            6242..7024
                     /locus_tag="VFR20_11125"
     CDS             6242..7024
                     /locus_tag="VFR20_11125"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEU0231115.1"
                     /translation="MRKSDGGLAGLKRLQSLQARTDEVRAPARTLGKKRRRGARPQAE
                     TLRASPETVPVGHTTSLSAAPTAPVTEPPPLTVEDAVLFKRAMRSVTPIRTTGRIILP
                     PRPVPPKTLLRQRRLRAAGSEPAPLAAVSDAFAPAATAHDATSFVKAGRGPDLIRDLR
                     RGKWPMQASLDLHGSTLDEARTRLDRFLVSCLSHGIRSVQIVHGKGLGSKDGPVLKQT
                     VRRWLTQIDAVIAYVECADVHGGSGAVQVLLRAPEKPTQLAD"
     gene            7052..7381
                     /locus_tag="VFR20_11130"
     CDS             7052..7381
                     /locus_tag="VFR20_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEU0231116.1"
                     /translation="MSWLLLTIAITAEIIATTSLKASDGFTRLYPSLITVIGYVISFY
                     CLALTLRVIPVGVVYAIWSGVGIVAISIIGYVVFRQALDGAAMAGIGLIVAGVLVINV
                     FSSSVRH"
     gene            complement(7684..9369)
                     /locus_tag="VFR20_11135"
     CDS             complement(7684..9369)
                     /locus_tag="VFR20_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0231117.1"
                     /translation="MPELSLVHGATEPPLRTETIGRCLEHAATHWPDQEAVVSVHQGI
                     RITYEQLNSQADHIAAGLLALGLERGDRVGIWSPNKAEWALIQFATAKAGMILVNINP
                     AYRVSELEYTLNKVQIRALVAAQRFKTSDYVAMVEQLLPEVAVHEGMLTAAAAPHLRH
                     VIKIGGAPRPGWRLFDELATLGGAAEREQVRRIGGELSCHDPINIQFTSGTTGLPKGA
                     TLSHHNILNNGFFVGECVGLRQGDRLCIPVPLYHCFGMVMGNLGCLTHGAAMVYPSDA
                     FDPLSVLRTIQDERCVGVYGVPTMFIAELTHPEFKNFDLSSLRTGCMAGSPCPVDVMK
                     QVLGAMHMRDVTIAYGMTETSPVSFQSAANDDLETRVSTIGRVQPHLQCKVIDGEGNT
                     VPRGCPGELCTKGYSVMLGYWDDDEKTGEAIDADGWMHTGDLVVIDERGYGNVVGRLK
                     DMVIRGGENIYPREIEEFLYGHPAVRDVAVVGVPDEKFGEEICAWITLADGHTATEDE
                     IRAYCEGRIAHYKVPRYVRFVAEFPLTVTGKVRKFEIRDRMIEELHLTVRKTA"
     gene            complement(9485..10651)
                     /locus_tag="VFR20_11140"
     CDS             complement(9485..10651)
                     /locus_tag="VFR20_11140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0231118.1"
                     /translation="MDFSFSPDQEAIRDAIQKICARFDDQYWLEKDRHGGFPHELHQA
                     LARDGWLGICIPEAYGGAGLGITEAALMMQTISQSGAGLTGASAVHMNIFGLNPVVKF
                     GTEAQKQRMLPPLIEGREKACFAVTEPNTGLNTTQLKTRAVRRGDHYVLHGAKVWIST
                     AQVAEKMLILARTTDLEQVKRHTDGLSLFYTNLDRTRVDVREIEKMGRKAVDSNELFI
                     DGLEVPVDDLIGEEGQGFRYILHGMNAERILIASEAIGLGRAALARATDYARERVVFG
                     RPIGQNQGIQHPLAASWMELEAANLMVFHAATLFDAGLDCGAQANAAKYLAGEAGFKA
                     CQNAVMTHGGFGYAKEYHVERYLREVLIPRIAPVSPQLVLCYIAEKVLGLPKSY"
     gene            complement(10725..11459)
                     /locus_tag="VFR20_11145"
     CDS             complement(10725..11459)
                     /locus_tag="VFR20_11145"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU0231119.1"
                     /translation="MGVKTADRTLDVFEAFATAREPLVLSELAQRVGMPMSSCFALVR
                     TLQQRGYLYLPTARGGLYPTKKMVELSAAIAAHDPLLERIAPVLARVRDETGETAILS
                     KLQGTQIIYLEVCESQQSIRYIAHAGDFKTLHANSVGKAILGLMPTDERDTLLSSATF
                     APLTARTITSREAFDAELAESARRGWYASEGESVPDLMGVALALRIGTGYFALSVAGP
                     LSRIRAHAEAHAKVLDAARMAVISQG"
     gene            complement(11489..12718)
                     /locus_tag="VFR20_11150"
     CDS             complement(11489..12718)
                     /locus_tag="VFR20_11150"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU0231120.1"
                     /translation="MSEQLENKVPKPLDGVRILDMTAVVMGPYTTQILGDMGADVIKV
                     EAPRGDTMRRVGPMRHPDMGHMYLNSNRGKRSIALDLKNPGGRDTLLKLAATVDVLVY
                     NVRPKAMARLGLSYEDVRAVNDKIIYVGLVGYGQNGPYAEKPAYDDLVQGAVGMGSLM
                     ARSGSPAPRYVPLTLADRAVGLHGVIAITGALYFRTHTGQGQEIQVPMFETMASMVLA
                     DHMAGATFDPPLGATGYQRLLSPDRRPFQTRDGHVCVLIYTDAHWERFFQAIGQASVF
                     HEDARFTTMEARAENIDAVYKTVSDIMLMRTSGEWLELFEKADIPVMPMHTVDTLMDD
                     PHLRAVGLVETTRHPTEGVLRTVGTPATWSVSQPAASRPAPRIGEHSVEVLREIGCDQ
                     NTIAALISQGALIDGAA"
     gene            complement(12998..13945)
                     /locus_tag="VFR20_11155"
     CDS             complement(12998..13945)
                     /locus_tag="VFR20_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231121.1"
                     /translation="MALGVVLVNVLLFVGGAIHATLPWQHDSHAYRQALSTPSSLAAM
                     LVPLLCTAALVAALAWALARGRLELAPQSVTQPGRVFWTFMVVYVMLCGIDIYILSSH
                     IQQFRAAIWSWLGVHGIYLLRARIGWMTLIISLTSLAISVLIVLVACQVALRVGQPGN
                     AGASAPRTDTPARHTVPVACGVFFVSVQWLLQNAFNGWMDVYQHVHGLLALASGVVGP
                     LLVFGLAFTGARMGAGAPRQARPLRALAAALLTLTLQHAICIAIGVGWLMWAMHGAST
                     YFIRVSDLIGPVMLMAALYALLLVFFMRLFTRLFYRRLQ"
     gene            complement(14028..14408)
                     /locus_tag="VFR20_11160"
     CDS             complement(14028..14408)
                     /locus_tag="VFR20_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0231122.1"
                     /translation="MIIDPNLTILYVADTPVSERFYADLLGKRAVESSPAFAMFALDS
                     GHRIGLWARHTVQPTPAPRAQGDGVEIVFAVGSDAEVDAAHDDWSRRGLSIAQKPTVM
                     EFGRTFVALDPDGHRLRVYHHAES"
     gene            complement(14577..15347)
                     /locus_tag="VFR20_11165"
     CDS             complement(14577..15347)
                     /locus_tag="VFR20_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEU0231123.1"
                     /translation="MAVYIGHDFLALPLVLQWRRHNLEASNVSRSERLLALLQLLRSR
                     RHPVPGAELSRSLGVSLRTIYRDIGTLQEQGARIEGEAGLGYVLRPGYLLPPLMFTEE
                     EIEAMVLGSRWVMRHADSGLSESARSALARIASVVPRSLRDTLDAATLLIASQPRVPP
                     PEVDVADIRLAIRGERKMFLRYQSLSDQESTRTVWPFAMGYFDGVCLLVAWCETRNDY
                     RHFRIDRIQHVEVMTERYPSFRHVLLKGWRERQGIAPE"
     gene            15387..16982
                     /locus_tag="VFR20_11170"
     CDS             15387..16982
                     /locus_tag="VFR20_11170"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU0231124.1"
                     /translation="MSVTPPSPENPASIEPTRTGPALHTPWLTLFICIACIGVYLWQG
                     WTLGHYSNLSGADLAILGGNVPSLSLGPQPWRLASSIFLHESPIHLIFNLAGMLLIVA
                     VLEALVARWKWVVLFLAGSLFSSFASACVNYGAHDVAFLGRYVHRLFVSVGISGAIMS
                     AGGALIVFAVRQWPAKDQETHKRILRGALAFTVINLIYGFTQSGVDNTAHVYGLIFGV
                     LGGVALLLPHTSQARWMRGMVTGIFTLAFATIIAWISTGLWKRLPPAQERMLYLKDTL
                     HTIVLLRAERQAANGRALERRQKLDYVNESDAIARRIPVPNVAKIVPLSEDGKVLVAT
                     NGDASRIIKYDLKRGKPLRVVAKVDYPAGAGRGCPDPKCSAVGVSNLVIDGTGTYTYA
                     STLYRGSVSAIDLASGRIAYTLHTGTYPSCLLLRDGRLYAYDELDNTISVMDADGRHP
                     MVLLALSDAVLGISPASNSVRRVWQLDNNQNTWITKTMDGRRFYLPGSKNTQIFDMTR
                     SAAPGDIGRTYNTLAKIDQRQWQ"
     gene            17504..18970
                     /locus_tag="VFR20_11175"
     CDS             17504..18970
                     /locus_tag="VFR20_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU0231125.1"
                     /translation="MTHWPKAARRTWTTIHHATRRKARQLHRMSRRTLQFSLLLAGAA
                     MVSFMSLGFARMVDLALHWNKVWVAQVPWAIWIALPLGMVCLRWATLRFAPNAVGSGI
                     PQVIASLSLPARSGRITLVALYQSIWKIPLTFLGLVMGASIGREGPSVQVGAALMLAW
                     GRFCKKMGVPLKDVHTSELIATGAAGGLAAAFNAPLAGVIFAIEELGRGTALRWQRLV
                     LLGVLAAGFIVVAFAGNNPYFGTFVGQALKHDMPGWTLLCGLVCGVGGGIFSWLLSKG
                     AAAFAPARWRGWLRQHPLHLAFLMGLILAVLGLLTHYTVFGTGYDVASSAMAGHPLES
                     PGFGPAKLMATVVSYWAGIPGGIFTPALTAGAGFGAELSRLTGALADQRVLVLLSMAS
                     FLAAATQSPLTASVIVMEMTGSQPMLVWLLIASLCASLVSRLFSPQPFYHFSAGRFRQ
                     QVRENDIALAKAKAAPATDSGPDPSNTKDEPVGSTPRG"
     gene            19785..20264
                     /locus_tag="VFR20_11180"
     CDS             19785..20264
                     /locus_tag="VFR20_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU0231126.1"
                     /translation="MKPFKNLIERAWEGLSEGWRELLTRGSGSLTHFGTKDTDTVKDQ
                     STQEFPHWSLLAAETWETALSVVVRLEIPGMNKDDLVVDIHGNILRIRGKKLSSVVQD
                     GRRYHLMERAYGRFERSISLPHSVHPDQAEVSYRDGVLTVIAPKTDTLPPRRLTVSK"
     gene            complement(20493..22046)
                     /locus_tag="VFR20_11185"
     CDS             complement(20493..22046)
                     /locus_tag="VFR20_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEU0231127.1"
                     /translation="MDSAISLDQLDEQQLRTLVQQLMGEVAARDGQIATRDQVIHTKQ
                     THIDPLTHELVLYKRWRYGKRSEQLNAAQLSLLQETMDEDLAAIEEELDALRDAPAAT
                     PQKPRAPRRLRLPAELARVDIGHEPASTQCRCGCQMQRVGEDVSEKLDFVPGVFTVER
                     HIRGKWVCAQCETLTQAPVPPQIIDKGIPTAGLLAQVLVAKYADHLPLYRQEGIFARA
                     GVALPGSTLGDWVGVCGLRLQPLVDALKMEMLGRTVLHADETLVQMLKPGAGKTQRAY
                     LWAYTPTSYDSLRAVVYDFAPSRAGEHCRTFLQDWRGKLVTDDYSGYKAGFAAGITEL
                     GCMAHARRKFHDLHVNHQSQIAAQALELFAGLYGVEREASELPADGRRQIREELALPI
                     ADTLHRWLIAQRQRVPDGSGTARAIDYSLKRWEALTRYLDDGDAPIDNNWVENQIRPW
                     AIGRSNWLFAGSLRGGQRAAAIMSLIQSARLNGHDPYAYLKDVLTRLPSQKNHQIAEL
                     LPHRWVPAA"
     gene            complement(22166..22435)
                     /gene="tnpB"
                     /locus_tag="VFR20_11190"
     CDS             complement(22166..22435)
                     /gene="tnpB"
                     /locus_tag="VFR20_11190"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU0231128.1"
                     /translation="MIHVEAIWLATEPLDMRAGAGTAMARVVAVFGAARPHHAYCFAN
                     RRANRMKVLVHDGFGVWLAARRLNRGKFVWADAMHGPRIELDAAQ"
     gene            complement(22432..22770)
                     /locus_tag="VFR20_11195"
     CDS             complement(22432..22770)
                     /locus_tag="VFR20_11195"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU0231129.1"
                     /translation="MPQTELAPIRQRRSYPKPLKAQIVAECRQPGASVAGVALSHGIN
                     ANVVRKWIRQAEQLPVPAPAFVPIMPSGLPGVGRHIEIHLTRGAVQATVRWPVSEAGI
                     CVSWLREWLR"
     gene            23009..23449
                     /locus_tag="VFR20_11200"
     CDS             23009..23449
                     /locus_tag="VFR20_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU0231130.1"
                     /translation="MYRPLFVRDIFAELDRLQREMQEAFELSPSIRGLGRGGFPALNV
                     GGTPQSVEVYAFAPGLDPSTTEVSLDRGLLTIAGERKSELPSGDDDKATVHINERFGG
                     RFRRVVSVSDDVDPSTVSADYHDGVLHIHMKRQESAQPRRITVQ"
     gene            23559..23879
                     /locus_tag="VFR20_11205"
     CDS             23559..23879
                     /locus_tag="VFR20_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU0231131.1"
                     /translation="MPPVDVIEDSTGITLRADLPGVPKDGLKLQVESSTLTIEGEVSI
                     PMLESMEATYIEVGLPRFRRVFTLSKELDTGKVSAELNHGVLSLRIPKAEHAQPRRID
                     IKVS"
     gene            complement(24099..25355)
                     /gene="icd"
                     /locus_tag="VFR20_11210"
     CDS             complement(24099..25355)
                     /gene="icd"
                     /locus_tag="VFR20_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEU0231132.1"
                     /translation="MSYQHIKVPSAGQKITVNADFSLNVPNEPILPYIEGDGTGADIT
                     PVMLKVVDAAVAKAYGGKRKIHWMEVFAGEKSTRVYGPDVWLPEETLEAVKEYVVSIK
                     GPLTTPVGGGIRSLNVALRQQLDLYVCLRPVRYFKGVPSPVREPEKTNMVIFRENSED
                     IYAGIEFEAESANAKKLIAFLQNELGVKKIRFPETSGIGVKPVSSEGTKRLVRKAMQY
                     AIDNDRSSVTLVHKGNIMKFTEGAFRDWGYELAREEFGAQPIDGGPWCKFKNPKTGRD
                     ITVKDVIADAFLQQILLRPAEYDVIATLNLNGDYVSDALAAQVGGIGIAPGANLSDSV
                     AMFEATHGTAPKYAGKDYVNPGSEILSAEMMLRHMGWTEAADLIISSMEKSILSKKVT
                     YDFARLLEGATQVSCSGFGQVMIDNM"
     gene            25905..26333
                     /locus_tag="VFR20_11215"
     CDS             25905..26333
                     /locus_tag="VFR20_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEU0231133.1"
                     /translation="MFNPSRDQARQFFIDAWQKHRAANIVTPLEAMAVQWVEQHPEYH
                     ADLERPDALAAEYPVEQGRTNPFLHLSMHLAIAEQLSIDQPPGIVAAHRLLLATRDPH
                     QAAHEIMECLGEVVWEAQRLGRPFDNDTYLDLIRRRATRD"
     gene            complement(26446..26808)
                     /locus_tag="VFR20_11220"
     CDS             complement(26446..26808)
                     /locus_tag="VFR20_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU0231134.1"
                     /translation="MKKLLLALAGASLVAAGSVAHAGDIAAGKLAFQTHACASCHGAD
                     AKSPVSPAYPILAGQHADYIAHALHAYQRGQKGAPATANIRKNPIMGGMAMSLSSADI
                     SNIAAWISSLPSPLSNAR"
     gene            complement(26822..27178)
                     /locus_tag="VFR20_11225"
     CDS             complement(26822..27178)
                     /locus_tag="VFR20_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU0231135.1"
                     /translation="MKLRISFKRALSALAVAASCGLAGQAVAASAAPVGNAKAALGKT
                     SMCIGCHGIEGYKASFPEVYHVPKISGQNAEYIIAALKEYASGARTFPTMEAIAKSLS
                     PQDMADIAAYYSSLKK"
     gene            27323..>27715
                     /locus_tag="VFR20_11230"
     CDS             27323..>27715
                     /locus_tag="VFR20_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU0231136.1"
                     /translation="MPSQASALPVERFNGTDRYVATDDLKLAVNAALTLQRPLLIKGE
                     PGTGKTMLAEEVARALERPLIQWHIKSTTKAHQGLYEYDAVSRLRDSQLGDEKVRDIG
                     NYIVKGVLWQAFDAPEPSVVLIDEIDKAD"
BASE COUNT         5349 a         8675 c         8468 g         5223 t
ORIGIN      
        1 actggccggt gccgaacagc gtggcgccgt tgacaatgta aggggtgtag caatcggtgt
       61 agccatgttc gccggtctgc agatcgagca tgaactgcgc cagcgcgcga tgcagccggg
      121 ccatctggcc ccgcaacatc atgaaacgcg ccccggccag cttgcgcgcg gcctcgaaat
      181 ccagccccag gggttcgccc agcgcgacgt ggtcgcgcgt ttcaaacggg aacgcgcgcg
      241 ggcagccttg cccgtcaact tccacgccag gcgggaccca gcggcgcacc tcgacgttgt
      301 catcgctgga ggcgccggcc ggcacactgg cgtgtggcag gttgggcagc gtcatgagcc
      361 aatcgttgag ttcggactgc accccggcca gatcggccgc cagttgcttg agacgttccg
      421 gaatggcctg cgattcagcc agttcctggc tggcgtcctg tcccttggac ttgagctggc
      481 cgatgagctt ggagacggcg ttgcggcgcg cctgcagggt ttcggtctcg acttgcaccg
      541 ccttgcggcg ggcttccagc gtgttgaagc gttcggtgtc gaattgaacg ccgcgcaggg
      601 caaggcgctc gaccacggtc gatggatctt tgcgcagttg attggggtct agcatggatg
      661 cgatgtccgt agaggaatgg aatgcgtgcg gccgcgacgg agggacaatg ttgacgcagg
      721 ccaccttcgg gccagcacga ttatacaaag ggccggatca cccctccgac ataccatgca
      781 agaacactac cggttgagtc ggacgtccaa attcggatat gcggggaccc accgcggata
      841 ggcacgcaaa gcgctacccg ccttttttcg cctcgtcgtc caattggcgc aggaaagcca
      901 gcttctcggc gatcttgccc tcgagcccgc gcgggacggg ccgataccag ttgggtcggg
      961 ccatgccgtc gggcaggtag gtttcgcccg ccgcgtagcc gttcggctca tcgtgggcgt
     1021 accggtactg tcgtccgtaa ccgagctctt tcatgagttt ggtgggggcg ttgcgcaaat
     1081 gcaccggcac gtcgcgcgag gtatcctggc gcacgaaggc gcgtgcctgg ttatacgcca
     1141 tgtagccggc attgctcttg gcggcaacgg ccagatagat cacagcttgc gccagcgcca
     1201 attctccctc gggcgacccc agccgttcat aggtgctggc cgcctcattg gcgatctgca
     1261 tggcgcgcgg gtcggccagg ccgatgtcct cccaggccat gcggatgatg cgccgtgaca
     1321 ggtagcgcgg atcggcacca ccatcgagca tgcgtgtgaa ccagtacagc gccgcatcgg
     1381 ggtcggaacc ccgcaccgac ttgtgcaacg cgctgatctg gtcgtagaaa gcatcccctc
     1441 ctttgtcgaa acggcgcagg ttctgcgaca gcgcggtttc gagccaggcc gcatcgacca
     1501 gttcgcgttg cgcccccagc gcggcctcgg ccacgacttc gaccgcattg atcagccgcc
     1561 gcgcatcgcc atccgcccag gccgccagtt gagcgcgggc ttcgtcatcg aaacgtggcg
     1621 tgggtcgatg tccggtggcc cgggggccgt cggttccctg ggggtcatca cccggcgggg
     1681 gggttgaatc gaacatctcc agcgcccgat cgagcaaccc gcgcaggtca tcgacagaca
     1741 gcggctccag cacgtacacg cgggcacgcg acagcagcgc ggaattcacc tcgaacgacg
     1801 ggttctcggt ggtggcgcca atgaacgtga acaggccgct ttcgacatat ggcagaaacg
     1861 catcctgctg ggccttgttg aaccgatgca cctcgtcgac gaacaggatg gtgcgccgac
     1921 cctgcgcctg cgcggcctgg gccaggacga ccgcctcgcg aatgtccttg accccgccca
     1981 gcaccgccga aatggccagg aactgggcgt cgaaaccgtc ggccatgagg cgcgccagcg
     2041 tggtcttgcc cacaccgggc ggcccccaga agatcatgga gtgcggccgg ccagactgga
     2101 aggccacccg caacggcttg ccgggcccca gcagatgggt ctgccccacg acgtcgtcca
     2161 gggacttcgg gcgcatgcgc tcggccagcg gcgcatgcga tcccgcgaca ggcgaggtga
     2221 acaggttgcc ggactcaggc atggcggggc tcgtgcgcgg cgccggcgcc gcgattacat
     2281 gtgaacgaca tccacccctt tgggcagcgc gaaggtgaac tcgctggcgg gcagcgtggg
     2341 gttcgtctgg atgtcggaaa ggtcgatgcg cgtgatctga ccgaaggaat cgagcagttc
     2401 gaggcgcacc ggcttgttgt cgctgaagcc catgtcaaca tgggcgaagc cggcatcggc
     2461 cttgcgcggc gtggcccgca gccattgcag gccgtctttg tcggggagcg gcgtgacgac
     2521 gaaggcgtca tcgagcgagc ccgacccgaa caggatggcg gcgggcgacg ccccgatgga
     2581 cttgctgacg tcgcgctcga tgacctgcgc cagatcgggg tcgtactgat agaccgtctt
     2641 gccgtcggac acgatgagct gttcataagg cttcttgact tcccaacgga aacgcccggg
     2701 tcgccggaac gcgaaacgac cggattccgg gggcttggcc ttgcccgggt cgttgggcgt
     2761 gccaagcgtc tgctgcttga aggtgccggt ggccacgctc acgctggcca cgaactggcg
     2821 cagttggtcg cgtgcgctgg cggaccacgc cagcgccggg caggccgcga gacacagcgc
     2881 ggccaacaag tgtttagtct gcatcagagg cgtttcccgc aggaaccagg atttcacgat
     2941 tgccattgga ctgcatggcc gagaccaggc cggcctgctc catttgctcg agcaggcgcg
     3001 cggcgcggtt gtagccgatg cgcagatgac gctgcacgga agagatggac gcacgccggt
     3061 tcttcaggac gatttcgacg gcttgatcat ataggggatc ggattcggca ttggcgaatc
     3121 cggtgaccgc gctcacgcca tcgccggttt ctccttccag cgcgccatcc agcaggccgt
     3181 cgacatagtt gggttcgccc tgctctttca ggtagtccac cacgcgatgg acttcgtcgt
     3241 cgtgcacgaa ggcgccgtgc acgcgcgtgg gcaaccccgt tcccggcggc atgtacagca
     3301 tgtcgccctg gcccagcagg gtttcggcgc ccatctggtc gaggatggtg cgcgagtcga
     3361 tcttggacga cacctggaag gcgatgcgcg tgggaatgtt ggccttgatc aacccggtga
     3421 tgacgtcgac gctggggcgc tgggtggcca gaatcagatg gatgcccgcg gcacgcgcct
     3481 tctgcgccaa ccgcgcgatg agctcttcga tcttcttgcc caccaccatc atcaggtcgg
     3541 ccagctcgtc gatgaccacg acgatcatgg gcatcggcgc caggggctcg ggctcgtcgg
     3601 gggtgagcga gaaagggttg ggaatgggct cttcgcgctt gatggcgtct tgaatcttgt
     3661 tgttgtagcc ggccaggttg cgcacaccca gcttgctcat gaggcgatag cgtttttcca
     3721 tttcgcccac gcaccagttc agcgcgttgg cggcgtgccg catgtcggtg accactggcg
     3781 ccagcagatg cgggatgtcg gcgtagatgc tcatttcgag catcttcggg tcgatgagga
     3841 tgaggcgcac ctgcgtggcg tccgccttgt acagcagaga caggatcatg gcgttgatgc
     3901 caaccgattt gccggaaccg gtggtgccgg ccaccagcag gtgtggcatc ttgcccaggt
     3961 cggccaccac cgggttgccc gcgatgtcct tgcccagcgc catggtgagc atcgacgcgc
     4021 cggaatagta ggtttgcgac ccgatgatct cggacagccg caccatctgc cgcttgggat
     4081 tgggcagctc cagccccatg aggttcttgc cagggatggt ctcgaccacg cgtatgctga
     4141 ccaggctgag cgcgcgcgcc agatctttcg ccaggttgac gatctggctg cctttcacac
     4201 cggtggccgg ctcgatttcg tagcgcgtga tgacgggccc ggcctgtgcg gagaccaccg
     4261 tcaccttgac gccgaaatcg gacaacttct tttcgatgag gcgcgacgtg tactcgatgg
     4321 tttccggcga cacggtttcg atgttgtcgt cgggtgcgtc aagcagcgac acggcgggca
     4381 ggtcgccgga gccgccgagc tcggatggca tcgggaacag cgccttctgc ttttcctttt
     4441 caacccggac cgacttgggc accgacacga tggctggttc gatgcgcacg ggctgttcgt
     4501 gcaccagttg cttctgctga gccacgaccg attcgttgcg ctcggcgctc ttgacctcgc
     4561 ccaccttgcg atccgcgcgc gcggcccgga gttcgaacac gcgccgcacg gatttttcca
     4621 gcgcggtgcc cacgcgctcg gccacgaaca gccacgagaa cccgaagaac aggcttgcgc
     4681 ccaccgcgat gaacaccagc agcaacaacg tgcaacccgt gttccccagc gacagcgaca
     4741 tgacgtgcgc cagcaagcgg ccaagctggc cgcccggccc cgctggcagc gaggcgccga
     4801 actgcttgag ctgaagggat tcgatgccca tggaccccac gaacagcagc aggaagccca
     4861 acccggcctc ccagtgcacc cggggcaggg tctcgacctt gtgattgggc agcaacagac
     4921 tggtcagtcg gtagaacccc gccaccacgc gctgggccaa cagcaccacc cagagccagg
     4981 ccgagtagcc aaagaggaac agcagcaggt cggacacgta cgcgcccagc ctgcctcctt
     5041 gattggacgt ggaggtcgac agcacggaat gcgaccatgc cgggtcggcc gggttccagg
     5101 tggcaagcac cagcgccagc caagcggcca gcgcggcgaa gacgaaccag cgtgcttcgc
     5161 gcaccaggcc ggcgagccgc agttgcaagg gagaagggcc attgcgcaca ttgcgcgagg
     5221 agcgggggga cgtcgcgggt attctagcca tgcactcatt ataattgcgg gattgtcttt
     5281 tacggatttt ctgcaatgac gaccccgaaa cacgccaaag tcctgattct cggctcggga
     5341 ccggcgggct acagcgcggc cgtgtacgcc gcgcgcgcca acctgaaccc ggtcctggtc
     5401 accggcatcg agcaaggcgg gcagctcatg accaccaccg acgtcgacaa ctggcccgcc
     5461 gacgtggaag gggtccaggg ccccgacctg atgcagcgtt tccaggcgca cgccgaacgc
     5521 ttcaagaccg agatggtctt cgaccagatc gtgagcgtcg acttgtcccg gcgccctttc
     5581 ctgctgatcg gcgattcagg tacacactat acctgcgacg cgctcatcat tgccaccggc
     5641 gcctccgccc agtacctggg tctgccgtcg gaacaacagt tcatgggccg gggcgtgtcg
     5701 ggctgcgcga cgtgcgacgg ctttttctac aagaaccagg acgtcatcgt ggtgggtggc
     5761 gggaacaccg ctgtcgaaga ggcgctgtac ttgtccaaca tctgccgcac cgtcaccatg
     5821 gtgcaccggc gcgacacgtt ccgctccgag cccatcctga ccgaccggct gacggacaag
     5881 gccaggaacg gcaacgtccg gctcaagctg tttcacgaac tggacgaagt gctgggcgac
     5941 gattcgggcg tgaccggggt ccggctgcgc gacaaccgca gcggccagac cgaagacctg
     6001 gccgtcacgg gcgcattcat cgccattggc cacaagccca acaccgccat cttcgagggc
     6061 caggtcgaca tggtcaacgg ctacatcacg acccgcagcg gcctgaacgg tctggccacc
     6121 atgacctcgg tcgagggggt gttcgcggcc ggcgacgtgc aagaccacgt ctaccgccaa
     6181 gccatcacca gcgcgggcac cggctgcatg gccgcgcttg acgcccaacg gtggctggag
     6241 aatgcggaaa agtgacggcg ggctggccgg cctgaaacgc ctgcagtccc ttcaagccag
     6301 aacagacgag gttcgcgccc ccgcccgcac acttggaaaa aaacgccgtc gcggcgcgcg
     6361 gccacaggcg gaaacgttgc gcgcgtcacc cgagaccgtg cccgtgggcc acacaacctc
     6421 gttgtccgcc gccccaaccg cccccgtcac cgagccgccc ccattgaccg tcgaggacgc
     6481 cgtcctgttc aagcgggcca tgcgttcggt cacgcccata cgcacaaccg gacgcatcat
     6541 cctgcctccg agaccggttc ccccaaagac cctgcttcgt cagcgccggc tgcgggcggc
     6601 gggttcggag cccgcgccac tcgcggccgt ctcggatgcc ttcgcacccg ccgccaccgc
     6661 gcacgatgcc accagcttcg tgaaggcggg ccgaggcccg gacctgatcc gtgaccttcg
     6721 gcgcggcaaa tggcccatgc aggccagcct cgatctgcat ggcagtacgc tggacgaagc
     6781 gcggacgcgg ctggaccggt tcctggtttc gtgcctgtca catggcatcc gcagcgtgca
     6841 gatcgtccac ggcaaggggc tgggttcgaa ggacggcccg gtcctgaagc agaccgtcag
     6901 gcgctggctc actcagatcg atgccgtcat cgcctacgtc gagtgcgcgg acgtccatgg
     6961 cggatcgggc gccgtgcagg tgctgctgcg cgcaccggag aagcccacgc aattggctga
     7021 ttgatcggac acgtagcaaa aggatgcacc catgagctgg ctcctgttga ccatcgccat
     7081 caccgcggaa atcatcgcaa cgacctcact gaaagccagc gacggcttca cgcgccttta
     7141 cccgtcgctg atcaccgtca ttggctacgt catttccttc tattgcctgg cactcacgct
     7201 gcgggtgatt cccgtcggtg tcgtctatgc catctggtcg ggtgtcggca tcgtggcgat
     7261 ttcgatcatc ggctacgtgg tgttccggca ggcgctggac ggcgccgcca tggccggtat
     7321 cggcctgatc gtcgcggggg tgctggtgat caacgtattt tcaagctcgg tgcggcactg
     7381 acacagcgtc gcggaggcgc gccacacggc gatgagcagg tgatcgggcg gaggatgcgc
     7441 gcagcaggct tgctgaacgt tcgggacgga catgacaaag gccggtacga ataccggcct
     7501 ttgtcatgtt tttatttttt atgggacctg ctgctttatt atgtttgccg agccgctacc
     7561 tatttggcgc gccaccgact tgcccatgca gggcgggtgt ctcctcacct gcatagacaa
     7621 aattgtgcac gaacttcgcg cccacgacac ttagggaatg cactaagtct gccgagttgc
     7681 gcatcaggct gttttgcgca ctgttagatg aagctcttcg atcatgcggt cgcggatctc
     7741 gaatttacgc accttgcccg tgaccgtcag cggaaattca gccacgaaac gcacatagcg
     7801 cggcaccttg taatgcgcga tccgcccttc gcaataagcg cggatctcgt cttccgtagc
     7861 ggtgtgccca tcggccagcg tgatccaggc gcagatttct tccccgaatt tctcatcggg
     7921 aacgccgacc acggccacat ccctcactgc ggggtggcca tacaggaact cctcgatttc
     7981 gcgggggtag atgttttcac cgccgcggat gaccatgtcc ttcaggcgcc ccacgacatt
     8041 gccatagccg cgctcgtcga tgaccacgag atcgcccgtg tgcatccagc catcggcgtc
     8101 gatggcttcc cccgttttct cgtcgtcatc ccagtagccc agcatgaccg agtaaccctt
     8161 ggtgcagagt tcgccggggc acccgcgcgg cacggtgttg ccctcgccgt cgatgacttt
     8221 gcattgcaga tgcggctgca cccgcccgat ggtcgacacg cgggtttcaa gatcgtcgtt
     8281 ggccgcggac tggaaactga cgggactggt ttcggtcatg ccataggcga tggtgacgtc
     8341 gcgcatgtgc atggcgccca gcacctgttt catgacgtcg accgggcacg gcgaccccgc
     8401 catgcaaccc gtgcgcaggc tggacaggtc gaagttcttg aattcggggt gtgtgagctc
     8461 ggcgatgaac atggtgggaa cgccatagac gccgacgcag cgctcatcct ggatggtgcg
     8521 caggacggac aggggatcga aggcatcgga cggatacacc atggccgcgc catgcgtgag
     8581 acagccgagg ttgcccatga ccatgccgaa acagtggtag agaggaaccg ggatgcacag
     8641 gcggtcacct tgcctgagcc ccacgcactc gcccacgaaa aatccattgt tcagaatatt
     8701 gtgatgcgac agcgtcgcgc ccttgggcag accggtggtg ccgctggtga actggatgtt
     8761 gatcggatcg tggcacgaga gctcgccgcc tatgcgacgc acctgttcgc gttccgccgc
     8821 gccgccaagc gtggccagtt cgtcgaacag gcgccagccg gggcgcggcg ccccgccgat
     8881 cttgatgaca tgacgcaaat gcggcgccgc ggcggcggtg agcatgcctt cgtggacagc
     8941 cacttccggc aatagttgct cgaccatggc gacgtaatca ctggtcttga accgctgcgc
     9001 ggcgaccaag gcgcgaatct ggactttgtt cagcgtgtac tcgagctcgg acacccggta
     9061 ggccgggttg atgttgacca ggatcatgcc ggccttggca gtggcgaact gtatgagcgc
     9121 ccattcagcc ttgttgggag accagatgcc gacgcggtca ccgcgctcga ggccaagggc
     9181 gagcaggccg gccgcgatgt ggtcggcctg gctgttcagt tgttcgtaag taatacggat
     9241 gccctggtga accgacacca ccgcctcctg gtcgggccaa tgcgtggccg cgtgctccag
     9301 gcaccggcct atggtttcgg tgcgcaacgg cggctcggtg gcgccgtgta cgaggctgag
     9361 ttcgggcatg cgggtctcct tcgtgatgcg ggacgctttc cccgaagata ccgcaaaata
     9421 cgtctgacca gaatccgcac agacccggtg cgcggccctg tatcagccga cttccgccca
     9481 atgctcaata cgacttgggc aaccccagca ctttctcggc gatatagcac agcaccagtt
     9541 gcgggctgac cggggcgatg cgcgggatga ggacttcacg caagtagcgc tcgacgtgat
     9601 attccttggc atagccaaag ccgccatgtg tcatgaccgc gttctggcag gctttgaacc
     9661 cggcctctcc cgccaggtac ttggcggcgt tggcctgcgc accgcagtcc aggccggcgt
     9721 cgaacagtgt ggcggcatgg aacaccatca ggttggcggc ctcgagctcc atccagcttg
     9781 ccgcgagtgg atgctgaatg ccttggttct ggccgatggg ccgcccgaac accacacgct
     9841 cacgcgcgta atcggtggcg cgcgccagcg cggcgcgacc caggccgatg gcttctgagg
     9901 cgatcaggat gcgctcggcg ttcatcccgt gcagaatgta gcggaacccc tgcccttcct
     9961 cgccgatgag atcgtccacc ggcacttcca acccatcgat gaagagctca ttggaatcca
    10021 ccgccttgcg gcccattttc tcgatctcgc gcacgtccac gcgggtgcga tccagattgg
    10081 tgtagaacag gctgagcccg tcggtgtggc gtttgacctg ctcaagatcg gtggtgcggg
    10141 cgagaatcag catcttctcg gccacttgcg ccgtggagat ccagaccttg gcgccatgca
    10201 gcacataatg gtcgccacgc cgcaccgcgc gtgtcttgag ctgggtggtg ttcaggccgg
    10261 tgttcggctc ggtcaccgcg aaacacgctt tttcgcgccc ttcgatcagg ggcggcagca
    10321 tgcgttgctt ctgcgcctcg gtgccgaatt tcaccaccgg gttcaggccg aagatgttca
    10381 tgtgcaccgc cgacgccccc gtcaggcccg cgcccgactg gctgatggtc tgcatcatga
    10441 gcgccgcctc ggtgatgccc aggcccgcgc cgccataggc ctcggggatg cagatgccca
    10501 gccacccatc ccgcgccagt gcctggtgca gttcgtgcgg gaagccgccg tggcggtctt
    10561 tttccagcca gtattgatca tcgaaacgcg cacagatttt ctgtatggcg tcgcgtatcg
    10621 cttcttggtc cggggaaaag gaaaagtcca tctgagcaaa cctctgaatg agtcgtcctg
    10681 cgacaaacga agcaccacgc gcccgcgcgg tccgggaaaa agattcaacc ctggctgatg
    10741 accgccatgc gtgccgcgtc gagcactttg gcgtgcgcct cggcgtgggc ccgaatgcgc
    10801 gacagcggac ccgccaccga caaggcgaag taacccgtgc caatgcgcag agccaacgcg
    10861 acacccatga gatcgggaac gctttccccc tcgctggcgt accatccgcg ccgggccgat
    10921 tcggccagtt cggcatcgaa cgcttcgcgc gatgtgatgg tgcgcgccgt gaggggcgcg
    10981 aacgtcgcgc tcgacaacaa tgtgtcgcgc tcgtcggttg gcatcaggcc caggatggcc
    11041 ttgcccacgg aattcgcatg gagcgtcttg aaatccccgg cgtgcgcaat gtagcgaatg
    11101 ctctgttggg attcacagac ttcgagataa atgatctgtg tgccctgcag cttgctgagt
    11161 atggcggttt caccggtttc gtcgcgcacc cgcgcgagca ctggcgcgat gcgttcgagc
    11221 aacgggtcgt gggcggcgat ggccgctgac aattccacca ttttcttggt ggggtacagg
    11281 ccgccgcgcg ccgtgggcag atacaggtaa ccgcgctgct gcagggtgcg caccagggcg
    11341 aaacaactgg acatgggcat ccccacgcgc tgcgcgagtt ccgacaggac aagcggttcg
    11401 cgcgccgtgg cgaaggcttc gaacacatcc agggtgcggt cggcggtttt cactcccatt
    11461 gtgggctctc cgtgggtttg gcgggcggtc aggcggcgcc gtcgatcagc gcgccttgtg
    11521 agatgagggc cgctatggtg ttttggtcgc aaccgatttc ccgcagaacc tcgacactgt
    11581 gttcaccgat gcgcggggcc ggacgcgacg cggcaggctg ggaaacgctc caggtggcgg
    11641 gcgtgccgac cgtgcgcagg acgccctcag tcggatgacg cgtggtttcg accaacccca
    11701 cagcccgcaa atgcgggtcg tccatcaggg tatcgaccgt gtgcatgggc atgactggga
    11761 tgtcggcttt ttcgaagagt tccagccatt ctccactggt gcgcatgagc atgatgtccg
    11821 atacagtctt gtagactgcg tcgatgttct cggcgcgcgc ctccatcgtg gtgaaacggg
    11881 cgtcttcgtg aaagacgctg gcttggccaa tggcctggaa gaaccgctcc cagtgcgcgt
    11941 cggtgtagat cagcacacac acatggccat cgcgtgtctg gaatgggcgc cggtcgggtg
    12001 acaacaaccg ctggtagcct gtggcgccca gagggggatc gaacgtggcg ccggccatgt
    12061 gatcggccag caccatcgag gccatggttt caaacatggg cacctggatt tcctggccct
    12121 ggccggtgtg ggtccgaaaa tacagcgccc cggtgatggc aatgacccca tgcagaccca
    12181 cggcccggtc ggccagagta agcggcacgt accgcggcgc cggcgaaccc gaccgggcca
    12241 tgagcgaacc catgcccacg gccccctgca ccaggtcgtc ataggcgggt ttttcggcat
    12301 acggaccgtt ctgaccatag cccaccagac ccacgtatat gatcttgtcg ttgaccgcgc
    12361 gcacgtcttc atatgacagc ccgagccgcg ccatggcctt gggccgcacg ttgtacacca
    12421 ggacatcgac cgtggcggcc aatttgagca aggtatcgcg gcccccgggg ttcttgaggt
    12481 cgagcgcgat gctgcgcttg ccgcggttcg agttcaggta catatgaccc atgtcgggat
    12541 gccgcatggg ccccacccga cgcatggtgt caccgcgcgg cgcctcgacc ttgatcacgt
    12601 cggcgcccat gtctcccagt atctgggtgg tgtatggccc catgaccacg gcggtcatgt
    12661 ctagaatgcg gacaccatcc aggggtttgg gcactttgtt ctccagttgt tcactcatcg
    12721 cgggcccggc accgcccagg gaccacggtg cggcttggcc aattaaattc acatatgtga
    12781 attattattc cgtatgcgaa taataatcgc agatcgccgc atcaatgtca aacaatcacc
    12841 agtcctccac gcatagggaa accacaccac ggcctcgtca cagagatggt gtcaagggaa
    12901 tcgatagaat ttggctcccg gggttaccgt accccgcgcc cggcgatata tccccagatc
    12961 gcgggatacc ccctgttcgt cgtcacaccg cgtgaggcta ctgcagccgc ctatagaaca
    13021 gtcgtgtgaa caatcgcatg aaaaaaacca gcaacaacgc gtacaacgcg gccatcagca
    13081 tcaccggccc gatcagatca ctgacacgga tgaaataggt gctagcgcca tgcatcgccc
    13141 acatgagcca acccacgcca atggcaatgc aaatggcgtg ctgcagcgtc agtgtgagca
    13201 gtgcggctgc cagcgcgcgc aacggccgtg cttggcgcgg cgcgcccgcc cccatcctgg
    13261 cgccggtgaa cgccagcccg aacaccagaa gcggccccac caccccgctt gccagggcca
    13321 gcaggccatg aacgtgctga tagacatcca tccagccatt gaatgcattc tgcagcagcc
    13381 actggaccga tacgaaaaac acaccgcacg cgaccggcac ggtatggcga gccggtgtat
    13441 cggttcgcgg cgccgaggct ccagcgttgc ctggctgacc gacacgtagc gcaacctggc
    13501 aggccaccag cacgatcagc accgaaatcg cgagactcgt caggctgatg atcagcgtca
    13561 tccacccaat ccgcgcacgg agcaggtaaa tgccatgaac cccaagccat gaccaaatcg
    13621 cagctcgaaa ctgctgtatg tgcgacgaca ggatgtatat atcgatgcca cacaacatca
    13681 catacaccac catgaacgtc cagaacacac gccccggttg cgtcacgctt tgcggggcaa
    13741 gctcgagccg gccgcgcgca agcgcccacg ccagcgccgc gaccaatgcg gcggtgcata
    13801 acaacggaac cagcatcgcc gccagcgacg acggggtcga cagcgcttga cgataggcgt
    13861 gactatcgtg ttgccatgga agcgtggcgt ggatggcacc accaacgaac agcaggacgt
    13921 tcaccagaac gaccccaagg gccatgttcc gcggcaaccg ctggattcgt gcgagtaagg
    13981 gtgcgtttgt ctgtttattg gtggcgatca tgctcgtttg tgcggaatca agattccgcg
    14041 tgatggtaca ccctcagtcg gtgaccgtct ggatccaatg cgacaaaggt tcggccaaac
    14101 tccatgaccg tgggtttctg ggcgatggac aagccccggc gagaccagtc gtcgtgggca
    14161 gcgtcgacct cggcgtcgct gcccacggcg aacacgatct cgacgccatc cccttgcgcg
    14221 cgaggtgcgg gcgttggctg tacggtatgt ctcgcccaca atccgatgcg atggcctgaa
    14281 tcgagcgcga acatggcaaa tgcgggcgag ctctcgactg cgcgcttgcc cagcaggtcg
    14341 gcatagaagc gttcgctgac tggggtgtct gcaacgtaga gaatggtcaa attcgggtcg
    14401 ataatcatag gcctcctggg cgtggttgac tcggaaacgg gaaccaccgt ttcatgccca
    14461 caagcataaa caacgtgatt gtcagattct gtcagcagca tgctccccat caggaaacaa
    14521 cacgaccggc ttgctcatca acccctgccg ggacggtggc tcaggagaac cggggcttat
    14581 tctggcgcga taccctgacg ctcgcgccac cccttcagca ggacgtgccg aaaagacggg
    14641 tagcgttcgg tcatgacctc gacatgctgg atccgatcga tacgaaaatg gcggtagtcg
    14701 ttacgcgttt cacaccaggc caccaacagg cagacaccgt cgaaataccc catcgcgaaa
    14761 ggccagacgg tacgcgtcga ttcctggtca gacaggctct ggtagcgcaa gaacatcttc
    14821 cgctcaccgc gaatagcaag ccggatgtcc gccacgtcga cctccggcgg tggcacacgc
    14881 ggctgagagg cgatcagcaa agtggcggca tcgagagtat cgcgcaagga gcgcggcacc
    14941 accgacgcga tacgcgccag ggcgctccgg gcgctttccg acaggccgga atcggcatgc
    15001 cgcatcaccc agcgcgaacc cagcaccatt gcctcgatct cctcttcggt gaacatgagc
    15061 ggcggcaaca aataccccgg acgcaaaaca tagcccagcc ccgcctcgcc ttcgatgcgc
    15121 gcgccctgct cttgcaacgt gccgatgtcg cggtagatgg tgcgcaggct gacgcccaat
    15181 gaccgggata gttcggcgcc tggtacgggg tggcggcggc tgcgcaagag ctggagaagc
    15241 gccaataatc gttcggaccg tgacacgttc gatgcctcga ggttgtgacg ccgccattga
    15301 agcacaagag gcaaagcaag gaaatcatgc ccaatgtaga ctgccatgct ctacggtcct
    15361 atcagtttgt tcaccccagc atacccatgt ctgtcacacc accctctcct gaaaaccccg
    15421 cctcgatcga acccacccgg accggacccg ctctgcacac tccctggttg accctattca
    15481 tctgcattgc ctgtatcggg gtttacctct ggcaaggctg gaccctgggc cactacagca
    15541 acttgtcggg cgccgatctg gccattctgg gcggcaacgt gccgtctctt tcactcggtc
    15601 cgcaaccttg gcgtctggcg tcctcgattt ttctgcatga atccccgatc catctcatct
    15661 ttaatcttgc gggaatgctg ctcatcgtcg cggtattgga ggcactcgtc gcccgctgga
    15721 aatgggtggt acttttcctc gcgggtagcc tgttttccag ctttgcctcc gcctgcgtca
    15781 attacggcgc ccacgatgtg gcctttctgg gtcgttatgt gcatcgcctg tttgtcagcg
    15841 tggggatttc cggcgccatc atgagcgcgg gcggcgcgct tattgtcttc gccgtccgtc
    15901 aatggccggc aaaagaccaa gagacccaca agcgtatact gcgcggcgcg ctagcgttca
    15961 ccgtcatcaa cctgatatat ggcttcacgc aatcgggggt agacaacaca gcccacgtat
    16021 acggcttgat attcggcgtg ttgggaggcg tcgccctgct gctgccgcac acttcgcaag
    16081 ccagatggat gcgcggcatg gttaccggca tctttactct ggcttttgcc acaatcatcg
    16141 catggatctc gacaggcctc tggaaacggc tgccgcccgc ccaagagcgc atgctctatc
    16201 tcaaagacac cttgcacacg attgtgctcc tgcgggcgga gcgacaagcc gccaacggca
    16261 gagcgttgga acggcgacag aaattggatt atgtgaacga gtccgatgcc atcgcccggc
    16321 ggatcccggt tccgaacgtg gcgaaaatcg tgccgctgag cgaagatggc aaagtgctgg
    16381 tcgccaccaa cggtgatgcc tcgcgaatca tcaaatacga tctgaagcgg ggcaaaccgc
    16441 tgcgagtcgt cgcaaaggtc gactacccag ccggcgccgg ccggggatgc ccagacccga
    16501 aatgctcggc agtaggcgtc tccaacctgg tcatagacgg cacgggcacc tatacctacg
    16561 ccagcaccct gtatcgtgga tccgtctcgg ccatcgatct ggcttcgggc cgtatcgcct
    16621 acaccctgca cacgggaacg taccccagct gcctgctgct gcgcgacggt cgcctatacg
    16681 cctacgacga actggacaac acgatcagcg tcatggatgc cgatggcagg caccccatgg
    16741 tattgctggc gttgtcggac gctgtgttgg gcatctcgcc ggcaagcaat agcgtgcgtc
    16801 gggtatggca attggacaac aaccagaaca cctggatcac caaaaccatg gatggtcgcc
    16861 gcttttatct gcctggctcg aaaaacacgc aaatcttcga catgacgcgc agcgcggcac
    16921 ccggagacat aggtcgaacc tacaacacac tggcgaagat tgatcagcga cagtggcaat
    16981 gaaggcgcct gagttgaaac acacgcccga ttcgccacag cgcatttcct atcatgccaa
    17041 gagcctgaca agatcatagc caaccctgga caccatgaac ccgaccgctt tcgggccgcg
    17101 atgctttgca caacacctga aaagcgttgc gtgagcgcaa gcttaatatg cctcatccgt
    17161 ggcctgatcc accagactcg atggatgcag cgccgtactg ttcgacaaac acgttatcac
    17221 gccagttgcc ccttgccatc ctggataacc tggtgcctcc agcctgtacc gcttcggcga
    17281 aggcgctgac cgtgaactgg ttgcctatca gtattgacga tattggggcc acccgtagcg
    17341 ggtgccggcc gcccccaaca cttcaacagc atggacacca aaccttgcgt tgaacattgc
    17401 gtcgccaaat cacaatagct tttcctgcgc catcaattcc tcatgtgaaa aacccttctg
    17461 gcctatcaga taaaataacg gactgacaca acacggattg ctaatgaccc actggccaaa
    17521 agccgcacgc cggacctgga caacgatcca ccatgccacc cgacgcaagg ctcggcagtt
    17581 gcaccgcatg tcacgcagga cgcttcaatt cagcctgctg ctggctggcg cggcgatggt
    17641 ttcattcatg tcgctggggt ttgcccgcat ggtcgatctg gcgctgcatt ggaacaaggt
    17701 gtgggtggcg caggtgccct gggcgatctg gatcgcattg ccactgggaa tggtttgcct
    17761 gcgctgggcc actttgcgct ttgcgcccaa cgcggtgggc agcggtattc cacaggtcat
    17821 cgcgtccttg tctttgccgg cccgctcggg gcggatcaca ctggttgcct tataccagtc
    17881 gatctggaag attcccttga cattcttggg gttggtgatg ggtgcgtcca tagggcgcga
    17941 gggcccatcg gtccaggtcg gcgcggcact gatgctggca tggggccgat tctgcaagaa
    18001 aatgggcgtg ccgctaaagg atgtccatac aagcgagctg atcgccacgg gggcggcggg
    18061 tggtcttgcg gcagccttca acgcgccgct cgccggcgtc atattcgcta ttgaagagct
    18121 cggccgaggc acggcacttc gttggcagcg cctggtgctg ctgggcgtgc tggccgctgg
    18181 tttcatcgta gtggcgtttg ccggcaacaa tccgtatttt ggcacgttcg tcggacaagc
    18241 cctgaaacac gatatgccag gctggacctt gttgtgcgga ctggtctgtg gcgtcggcgg
    18301 cgggatattt tcctggctgc tcagcaaagg cgccgccgcg ttcgcgccgg cacgctggcg
    18361 cggctggcta cgccagcacc cccttcattt ggcatttctc atgggcctga tactggcggt
    18421 gctggggctg ctgacccact atacggtttt cggcaccggc tacgacgtgg catcatcggc
    18481 aatggccggg catccgctgg aatcacccgg cttcggcccg gccaagctga tggcaaccgt
    18541 ggtgtcatac tgggctggca ttccgggcgg gattttcacc cccgcgctga ccgccggggc
    18601 gggctttggc gcagagctga gccgcttgac aggcgcgctg gccgatcaac gcgtgctggt
    18661 gctgctgtcc atggcgtcat ttcttgccgc cgccacccag tctcccctga ccgccagcgt
    18721 tattgtcatg gaaatgaccg gcagccaacc aatgctcgtg tggctgctga ttgcctcgtt
    18781 gtgcgcctcg cttgtctcgc gcctgttttc accgcagccc ttctaccatt tttccgccgg
    18841 gcgttttcgc caacaggtgc gtgaaaatga tatcgccctg gccaaagcca aagctgcgcc
    18901 cgccacggac agcggacctg atccttcgaa cactaaagac gagccggtcg gctccacgcc
    18961 ccgtggttaa agcccctgat agacgcgata accaggccga atccgcaaag ttccggatgg
    19021 ataagcttcc ctatggctcc aaaaacgcga tggccccgta ttccaccagg ctcaaccgga
    19081 taccaaaagc cctgttttca ttcgaaagca ggactttttc gggccttgcg gcgcccgatc
    19141 gacaactatt acatgttgtt gatcaaaacc tgtgatctgg ttttatatgc atataaacca
    19201 gatatatagg atagttacaa tggcaccatg gcccaacaac ccgcttacgc cacccgcaag
    19261 gcccagcact ttcgcacggg cgatgagcaa gtcatcgccc acaaccccgc cttcatcttc
    19321 gacgaccctg aacggctgca acgcttcgcg caggaactcc agaaaacgga ggatttgtac
    19381 taccaggagc aactaacttt ttgggaatct gacccagcgg attcacgaaa atccggggat
    19441 catgtaagcc ggagcaacct tcgaacaggt gcccatgaac gaacccttct ccgaggccaa
    19501 ccgcatctgg gtggccccgt cccatgccgc gcgtctgggc gaggcagccg ccctttccga
    19561 gccatccctc tctaactatc ataaggcggc ggctgtccgc cgacgattcg acacctctac
    19621 cagcgcaagg aactctgaga gtggcgtctg caggccgagc aggccagaat tggtgcactg
    19681 caaaatatct ggaaaccttg caccttgcga gagtgcggga tggttgaaaa gcaatatact
    19741 cacttcgata tgggtttgcg gccagttcac tagggatcaa taccatgaaa ccttttaaga
    19801 acttaataga acgcgcgtgg gaaggactgt cagagggctg gcgggagttg ctcacacgcg
    19861 gcagcggatc gctgactcat ttcggcacta aagatacgga tacggtaaag gaccaatcga
    19921 cgcaggaatt tccgcactgg agtctgctcg ctgcggagac ttgggagacc gctctatcgg
    19981 tcgtagttcg cctagaaatt cccggtatga acaaagacga ccttgtcgtt gacatccacg
    20041 gaaacatcct gcgcatccgc ggcaaaaagc tctcgagcgt cgtgcaagat ggccggcgat
    20101 accaccttat ggagcgggcg tatggacgtt ttgaacgcag catctctcta cctcacagcg
    20161 tccatcccga tcaagccgaa gtctcctatc gcgatggtgt gttgacggtg atagcgccca
    20221 aaacagacac gctaccacca cgtcgcctta cagtatccaa atgagaggca cggctgaaat
    20281 gctgaaccag ggttctcggc tgcttcaacc tcgaagttta tgacgatcaa tatcgtcttt
    20341 agacctcctg gttgcaggca ataaaaatgt tgtcgttttc tgtggcttta agcgcgttgc
    20401 gcacgagatg tgcattgtgt ggcactgacg acgattatga actgccgctg atgctatgta
    20461 agcacccggt aaacccgtct tgacggtaga gatcaagcag cggggaccca tcgatggggc
    20521 agcaattcgg cgatctggtg gtttttctgc gatggcaggc gtgtgagcac atccttcaga
    20581 taggcgtacg gatcgtgccc gttcaggcgt gccgactgga tcaggctcat gatggcggcc
    20641 gcccgctggc cgccacgtag cgacccggca aacagccagt tcgagcgtcc gatcgcccag
    20701 ggccggatct ggttttccac ccaattgttg tcgatcggcg catcgccgtc gtccagatat
    20761 cgtgtcaacg cctcccaacg cttgaggctg tagtcaatcg ctcgtgccgt ccccgagcca
    20821 tcgggcaccc gctgacgttg tgcgatcaac caccgatgca gggtgtccgc gatgggtaag
    20881 gcaagctcct cccgtatctg cctgcggcca tccgctggca gttccgaggc ctcgcgctcg
    20941 acaccgtaca gcccggcgaa caactccagc gcctgggcgg cgatctggct ctggtggttg
    21001 acgtgtagat cgtggaactt gcgccgggca tgcgccatgc agcccagttc agtgatgcct
    21061 gccgcaaacc cggccttgta gccggagtaa tcgtcggtca ccagcttgcc gcgccagtcc
    21121 tgcaggaagg tacggcagtg ctctccggca cggctgggcg cgaagtcata gaccacggcc
    21181 cgcaggctgt cgtaggacgt gggcgtgtac gcccacagat acgcccgctg cgtcttgccc
    21241 gcgccaggct tgagcatctg caccagcgtc tcgtcggcat gcagcaccgt gcggcccagc
    21301 atctccatct tgagcgcatc gaccaggggc tgcagacgca gcccgcacac gcccacccag
    21361 tcacccagcg tcgagccagg cagcgccacc ccggcacgcg caaagatgcc ttcctggcgg
    21421 tacaacggca ggtggtcggc gtacttggcg acgagtacct gtgccagcaa gccagccgtc
    21481 gggatgccct tgtcgatgat ctgagggggt accggtgcct gtgtgagcgt ctcgcactgg
    21541 gcgcataccc acttgccacg gatgtggcgc tccacggtga agacgcctgg cacgaagtcc
    21601 agcttctcgc tgacatcctc gccgacgcgc tgcatctggc aaccacaccg gcattgtgtc
    21661 gaggctggtt catggccgat gtcgacacgc gccagctcgg cgggcagccg caggcgacgc
    21721 ggggctcgtg gtttctgtgg cgtggccgcg ggcgcgtccc gcaaggcatc cagttcttcc
    21781 tcgatggccg ccagatcctc gtccatcgtc tcttgcagca gactgagctg ggcggcattc
    21841 aactgttcgc tgcgcttgcc gtagcgccag cgcttgtaca ggaccagctc atgtgtgagc
    21901 gggtcgatgt gcgtctgctt ggtgtggatc acctgatccc gtgtggcaat ctgcccgtcg
    21961 cgcgccgcca cttcgcccat cagttgctgg accaatgtgc gcagttgctg ctcgtcgagc
    22021 tgatcaaggg agattgccga atccataggc ggcagtatgc cgccgtccgt cttgcgatga
    22081 tattggcgtc ttgcctgatt tacagcaccg agatggcgcc ccccgaaccc acatgctgcc
    22141 acggcaggcc cagcaccagg gcgtgtcact gcgcggcatc gagctcgatg cgcggcccat
    22201 gcatcgcatc ggcccagacg aacttgcctc ggttcaggcg ccgcgccgcc aaccacacac
    22261 cgaagccgtc atggaccagc accttcatgc gattggcccg gcgattggca aagcaatatg
    22321 cgtgatgcgg gcgggccgca ccgaataccg ctaccacacg cgccatagcc gttcccgcgc
    22381 ctgctcgcat atccagcggt tcagtcgcca gccaaatggc ttcgacgtga atcatcgcag
    22441 ccactcgcga agccacgaca cgcagatccc cgcttcgctg acaggccacc gcaccgttgc
    22501 ctgcacggca ccacgggtca ggtgaatctc gatatgacga ccaacgccgg gcaatccaga
    22561 tggcatgatc ggcacaaatg ccggtgccgg gacagggagt tgctcggcct gccggatcca
    22621 tttgcgcacc acgtttgcat tgatgccgtg cgatagcgcg acgcctgcca cggacgcgcc
    22681 aggttggcgg cactcggcca caatctgggc cttcaggggc ttgggatagg accggcgttg
    22741 gcgtatgggg gctaattctg tctgcggcat ggcgtccacc taaaaatggt ggacactatc
    22801 ggaagcaccc cgaatggctc aagacgggtt taccgggtgc ttacgatgct atcgcgtagc
    22861 gcttgtgtga gcagatcaag tgcaggttat ggttgccatg ttgtcggttg ggctcttgaa
    22921 atgttctgga tcgatcccca aaataagaac tggaagcgcc acggctccta ccggggcgtt
    22981 tggttcaaca gtaatagagg agagtctcat gtaccgacct ttgtttgttc gcgacatatt
    23041 cgccgagttg gatcgtctgc agcgcgagat gcaggaggcg tttgaacttt cacccagcat
    23101 ccgcggtctt ggtcgcggcg gtttccccgc gttaaatgtg ggcggaacgc cacaatcggt
    23161 ggaggtctac gcctttgcac caggtctcga tccttccacg acggaagtga gcctggaccg
    23221 tggcctgcta actatcgccg gtgaacggaa atcagaactt ccttcggggg acgatgataa
    23281 agccaccgta cacatcaatg agcgttttgg cggccggttc cggcgagttg tgagcgtgtc
    23341 cgacgacgtg gatcccagca ctgtatccgc cgactaccat gacggtgtgc tgcacattca
    23401 catgaagcgc caagaatcgg cccagcctcg gcgcattacc gtgcaatgat atcggagaat
    23461 caatcatgag cgatacgact gcaattacca aaaacgaaca gacttcccgc gttgcgccga
    23521 cgcgaaacga cacgactcag cgcaacgatg aggccctgat gcctccggtg gatgtgatcg
    23581 aagacagcac cggtattacg ctacgtgctg atctgccggg cgtgcctaaa gacgggctca
    23641 aactgcaggt ggagtccagc acgctgacga ttgagggtga agtgagcatt cccatgttgg
    23701 aaagcatgga ggcgacttat atcgaggtcg gcctgccccg cttccggcga gtcttcacgt
    23761 tgtccaagga actcgatacg ggcaaagtgt ccgccgaact caaccatggt gtgctgagcc
    23821 tgcgtattcc gaaggccgag catgcccagc cgcgccgaat cgacatcaag gtaagctaag
    23881 acgagccgcc gcccttcgcg tgaggggcgg cgtcagcccc gaggccttcg agcaggccgc
    23941 ggcgtgaggc gggcaagtgt ctcaaaacac cagcatcctg tccgaatgac agcagccacc
    24001 tctcacgaat tcagccatac cccaccggat accaaaaaac cccgccttcc tatgaagacg
    24061 gggcttttcc agaccttaaa ggatcaggct aaaaacactt acatgttgtc gatcatgact
    24121 tgaccaaagc ccgagcatga cacctgggtg gcaccttcga gcaggcgcgc gaagtcgtac
    24181 gtgaccttct tcgacaggat ggacttttcc atgctggaaa tgatgaggtc ggcggcttcg
    24241 gtccaaccca tgtggcgcag catcatctcg gccgagagga tttccgaacc ggggttgacg
    24301 tagtccttgc ctgcgtactt gggtgccgtg ccgtgggtgg cttcgaacat ggccacggaa
    24361 tcggacaagt tggcacccgg agcgataccg atgccgccaa cctgggccgc cagggcatcg
    24421 gacacgtagt cgccattgag gttgagcgtg gcgataacgt cgtattcagc cgggcgcagc
    24481 aggatttgct gcaggaaggc gtcggcgatg acgtctttga ccgtgatgtc gcgaccggtc
    24541 ttggggttct tgaacttgca ccacgggccc ccatcgatgg gttgcgcgcc gaattcttca
    24601 cgagccagct catagcccca gtcgcggaac gcgccttcgg tgaacttcat gatgttgccc
    24661 ttgtgcacca gcgtgaccga ggagcggtcg ttgtcgatgg cgtactgcat ggccttgcgc
    24721 accaggcgct tggtgccttc gctggagacc ggcttgacgc cgatgcccga ggtttccggg
    24781 aaacggatct tctttacgcc gagctcgttc tgcaagaacg cgatgagttt cttggcgttg
    24841 gcgctttcgg cttcgaactc gatgccggcg tagatgtcct ccgagttttc acggaagatg
    24901 accatgttgg ttttttcagg ttcgcgcacg ggcgacggta cacccttgaa gtagcgcacc
    24961 ggacgcaggc agacatacag gtcgagttgc tggcgcagcg ccacgttgag cgaccggatg
    25021 ccaccgccca ccggcgtggt cagaggcccc ttaatggaca cgacatattc cttgaccgct
    25081 tccagggttt cctcgggcaa ccagacgtcg gggccataga cgcgggtgga cttttcaccg
    25141 gcgaaaacct ccatccagtg gatttttcgc ttgccgccat aggccttggc cacagccgca
    25201 tccacgactt tgagcatcac cggcgtgatg tccgcgccgg tgccatcgcc ttcgatgtag
    25261 ggcaggatgg gctcgttggg gacgttcagc gaaaaatcgg cattgaccgt gatcttctgg
    25321 ccagccgagg gaaccttgat atgctgatag gacatgggtg ctcctgttgc tccggaaatc
    25381 gaatgaggtg aaaccacgcc aggcatggcg ccggcaaccc gcccagccac gcctgtgcgg
    25441 ccgagcaaat gaaaactgaa tgaaaaacaa tgtggcgccg cgcctggcgc gctatcccgc
    25501 aaaacaccgc gggacagcgc gccagcgggc gacgagacgc cattatgggc gcaaacccgc
    25561 ccccaataca acgaacacgg cccaacagcg gcaagtctta tataagagtg ctagtgtagc
    25621 gtactttcgg cacgctttta cggtggtgtc ctctcttgaa tgccactacc ccctccccgg
    25681 gcgccagtgt gccacttggt tggttcgagc agtcaaattc agaagcgagg caaccagata
    25741 gcgctgattc gggtgctatc gcggggattc accatgcggc ggacacgtcc aacgcacaga
    25801 tacatgcggt gcggtgacct gctcatggga ttcctgacag cggcagactc ggacggaccc
    25861 ggcccgcaat cgttaaaatg cttttttaaa gttcgagctc ttccatgttc aatccgtcac
    25921 gcgaccaggc ccggcagttc ttcatcgatg cctggcaaaa gcacagggcc gccaacatcg
    25981 tcacacccct ggaggccatg gcggtgcaat gggtcgaaca gcatccggaa tatcacgccg
    26041 atctcgaacg ccccgatgcg ctggccgccg aatacccggt cgagcaaggc cgcaccaacc
    26101 ctttcctgca tctgtccatg catctggcga tcgcggaaca attgtccata gatcaaccac
    26161 ccggcatcgt ggcggcgcac cggctcctgc tggccacgcg cgacccgcac caggcggcgc
    26221 atgaaatcat ggaatgcctg ggcgaagtgg tatgggaagc acagcgcctg ggcaggccat
    26281 tcgacaacga tacgtacctg gacctgatcc gccggcgcgc cacgcgcgac tagtgtcctg
    26341 ttagtcaaac agaacactag gcatgacgtc cgccaaaaaa acaaaccgcc cccaccgtgc
    26401 gataacggcg ggggcggttt gttttcgcgc gcggtagtcg gccgcctacc tggcgttgga
    26461 caggggcgat ggcaggctgg agatccaggc cgcgatattg gagatgtcgg cgctggacaa
    26521 cgacatggcc atgccgccca tgatgggatt cttgcggatg ttggctgttg ccggcgcgcc
    26581 cttctgccca cgctggtacg catgcagcgc atgggcgata tagtcggcat gctggcccgc
    26641 caggatgggg taggccgggc tgacggggga tttggcatcg gcgccatggc acgatgcaca
    26701 ggcgtgcgtc tggaaggcga gcttgccggc cgcgatgtcg cccgcatgag ccacggaccc
    26761 agcagcaacc agggaggcgc ccgccagagc aaggagaagt tttttcattt gggcacccgg
    26821 attatttctt gagactggag tagtaagccg ctatgtcagc catgtcttgc ggggacaggc
    26881 tcttggcaat ggcttccatc gtcgggaaag tgcgggcgcc gctggcgtat tccttgagcg
    26941 cggcgatgat gtactctgca ttctgaccag atatcttcgg cacatggtag acctctggaa
    27001 agctggcctt gtagccctcg atgccatggc aaccgatgca catggacgtc ttgcccaatg
    27061 cggctttcgc atttccaacc ggcgcggcgc tggctgcgac agcctggcct gccaacccac
    27121 aggaggccgc tacggccaat gcggacaacg cgcgcttaaa ggatattcgc aacttcatgt
    27181 tagctcttgt ttggcggtag atgaaaagtc tatcaatgac gagtcgaatc gatgatccgt
    27241 tgagattgta cgataattgt agcgaaccta tacagaacag cccttcggcc cgcatggcgg
    27301 gccaacacaa cggaacgaga ccatgccctc tcaagcctct gcacttcccg tcgaacgttt
    27361 caacggaacc gaccgctatg tcgccaccga cgacctgaaa ctggccgtca acgcggcgct
    27421 gacgttgcaa cgcccgctgc tcatcaaggg cgagcccggc acggggaaga ccatgctggc
    27481 cgaggaagtg gcgcgggcgc ttgagcgccc attgatccaa tggcacatca aatcgaccac
    27541 gaaggcgcac cagggtctgt acgaatacga cgccgtgtcg cgcctgcgcg actcgcaact
    27601 gggtgacgag aaagtacgcg acatcggcaa ctacatcgtc aagggcgtgc tgtggcaggc
    27661 tttcgacgcc cccgagccat cggtcgtgct gatcgacgaa atcgacaagg ccgac
//
LOCUS       DASTNK010000147         5551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_683602, whole genome shotgun sequence.
ACCESSION   DASTNK010000147 DASTNK010000000
VERSION     DASTNK010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683602"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VFR20_11235"
     CDS             <1..853
                     /locus_tag="VFR20_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0231137.1"
                     /translation="FVTAFGLRASRDGDEIELRATGSDHRWARILPSGAKSLAYLSFN
                     CYDTDFPIILNQLKACGATRSKSEEHVDGDGFWFHDPDGNLLQLRVGPKTSPARKGVA
                     TMAVTAPGERGVLGRAHRPRTTPTRLSHVLMFTPDLDRALRFYGEGLGLRLSDRSRDI
                     VAFMHARHGCDHHLVAFVRNAFKGWHHSAWDVPGIEQIGCGAEQMREAGFGEGWGLAR
                     HTLGSNYFHYVRDPWGSFWEYSADIDYVPAGSVWPGGDYPPEDALYMWGPAMPHYFIE
                     NTEHTGM"
     gene            complement(897..1598)
                     /locus_tag="VFR20_11240"
     CDS             complement(897..1598)
                     /locus_tag="VFR20_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0231138.1"
                     /translation="MTSDFEAFKAQLNLTRDEIYRELFRENIGLIRIKKEDVAVRNLG
                     RIIESTLKLASVKGFHAMSLRDLCADSGLSIGGVYAYIKNKDDLTHLIQSHGFILTRR
                     TLLACTAGMAPGPDKLFAAVRAHLYLSEMMPAWFSFSFMEAKSLPKKEKLEAVTTELE
                     IEGILFGIIQEGITAGKFRRVDARLLASLTKAMMQDWYLKRRKHRNQGTDVTHYARFV
                     WSVLDCYLRDATVKR"
     gene            complement(1653..2378)
                     /locus_tag="VFR20_11245"
     CDS             complement(1653..2378)
                     /locus_tag="VFR20_11245"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEU0231139.1"
                     /translation="MQKVHVIFRKDDLDPALAPGKIAVVLDVIFATSTIVTALREGAR
                     SVVPARDEAQARELSQGFPPGEFVLAGEYHADTLPGFHPYNPLAMRSSVLSGKDLIYA
                     TTNGTVALHGAMACKQVYAGSLLNASALADHLLAHYTTETLLLVCSGSRGTFSLEDFY
                     GAGCIVSRLLERSDPGRFALSDSAIAALRLCRENDALDILMASKVGRRMAARDMEDDI
                     RYAATLDACPIVPALMDGKLVRV"
     gene            complement(2482..4104)
                     /locus_tag="VFR20_11250"
     CDS             complement(2482..4104)
                     /locus_tag="VFR20_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0231140.1"
                     /translation="MPDNKYRTGLDKQAANYSALTPLSFLKRSAWVFPHKTAVVDDDG
                     ALTYERLYERCRRMASALVSLGIRPADTVSVLSFNSHELLESHYSVPMAGAVLNALNT
                     RLDAAALRFILQHGEARALLYDTEFEPLVKEVVGGMDAPPILVAIARVAPASPGFAAH
                     DYETLLASGDPDFEWRKPEDEWDAISLNYTSGTTGDPKGVVYHHRGAYLSAMSNAMAF
                     NMTPDTVYLWTLPMFHCNGWCYTWAIAAVGGTQVCLRKVQSDAVLERIRQYHVTHMCG
                     APVVLNLLVNDYSRQGVSLPEKVQFALGGAAPPSAVIGRAGKVGFAITHLYGLTESYG
                     PSTVCVWQNDWANLDNDSLAAKMARQGVGLYAIDDVQVLKADDDTPVPADGKTLGEIV
                     HRGNTLMKGYLKNPASTQRAFHNGWFRTGDLAVMHPDGYVEVKDRAKDIIISGGENIS
                     SKEVEDVLYQHPQIFEAAVVARPDDHWGEVPCAFVCLKEDAPEITEQDILDFCRAGMA
                     AYKVPKSVVFGPLPKTSTGKIRKNILREQARG"
     gene            complement(4129..>5551)
                     /locus_tag="VFR20_11255"
     CDS             complement(4129..>5551)
                     /locus_tag="VFR20_11255"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU0231141.1"
                     /translation="GADAVEVLEFARLAGGAIQDNHALTVMLTGGTHPGRLSVVVRSD
                     APSRIAASLTRAQEFHVLAAAHEAGVTVPRPLWLCEDESVAGHVFCVMERVAGSASPR
                     RLLRGALGDDQARALTRRLGVELARLHQVHPPRADLGFLPIPSGNPALWRVGTYRAAL
                     ADIPQPHPVLEWALDWLRDHAPVPGNLALCHCDFRTGNYMVDDGRLTAVLDWEFATWS
                     DPYEDLGWLCCKSWRFGANDHEVGGLGDKADLFEGYAGQAGHEVDAERVRYWEIMGFV
                     RWAVIALQQAQRHLSGEQLSLELALTGRMVPEMEFDLLSQIRGMEAARGADTDMACRT
                     GADEGFGSGHPRSLPATATTNLPSGADLLDVARDVLLNQLASLLPSDKTYEAKMVARA
                     MAVAARELKQRDATDQDGEARIAAFLREADLDAIDTTPSEHTLARLLRNGDIPSKRRE
                     ALSVLLMALTRAKLAINNPRYLS"
BASE COUNT          990 a         1717 c         1761 g         1083 t
ORIGIN      
        1 gttcgtcacg gccttcgggt tgcgcgcatc acgggatggt gacgaaatcg aactgcgcgc
       61 cacaggcagt gatcaccgat gggcgcggat tctgccgtcc ggcgcaaaga gcctggccta
      121 tctcagcttc aactgctatg acaccgattt tccaatcatt ctgaatcagt tgaaggcttg
      181 tggcgccacg cgttccaaga gcgaagagca cgtcgatggc gatggtttct ggtttcatga
      241 cccagacggt aacctgctgc agctgcgcgt cgggcctaag accagcccgg cgcgaaaggg
      301 cgtcgcaacg atggcggtca cagcgcccgg cgaacgcggc gtgttgggcc gcgctcatcg
      361 gccccgcacc acgcccacga ggttgtccca cgtgttgatg ttcacaccgg acctcgatcg
      421 cgctctgcgt ttctatgggg aaggcctggg gcttcgtctg tcagaccgtt cgcgcgacat
      481 cgtcgcattc atgcacgccc gccacggctg cgatcaccac ctggtggcgt ttgtccggaa
      541 tgccttcaaa ggctggcatc acagcgcgtg ggatgtgccc ggcatcgagc agatcggttg
      601 tggggcggaa cagatgcgcg aggcgggttt cggcgaaggc tgggggctgg cgcggcacac
      661 gcttgggtcg aactactttc attatgtgcg tgacccctgg gggtcgttct gggagtactc
      721 ggccgatatc gactacgtgc cggcgggatc ggtttggcct ggcggagact accctccgga
      781 agacgcgctc tatatgtggg gcccggcaat gccgcattac ttcatcgaga acacggaaca
      841 caccgggatg tagcgcacac tgggacgttg gacacgccga cacgtggaac acactgtcag
      901 cgttttacgg tggcatcccg caggtaacag tccagcacgc tccagacgaa ccgcgcatag
      961 tgcgtcacat cggttccctg gttgcggtgt tttcgtcgtt tcaggtacca gtcctgcatc
     1021 atcgccttgg tcagtgacgc cagcaggcgc gcatcgacac ggcggaactt cccggccgtg
     1081 atgccttcct ggatgatgcc gaaaagaatt ccttcgatct ccaattcggt ggtcacggct
     1141 tcaagcttct cttttttcgg caggcttttg gcctccatga aggaaaagct gaaccaggcc
     1201 ggcatcattt cgctcaagta caggtgcgcc ctgacggctg cgaacagttt gtcggggcct
     1261 ggcgccatgc cggcggtgca tgcgagcaac gttcgccgcg tgaggatgaa tccgtggctc
     1321 tggatcaggt gggtcagatc gtccttgttc ttgatgtagg catagacgcc gccgatgctc
     1381 aggcccgagt cggcacacaa atcgcgcaac gacatcgcgt gaaagccctt gacgcttgcc
     1441 agcttgagcg tggattcgat gatgcgcccc aggttgcgca ccgccacgtc ttccttcttg
     1501 atacggatca ggccaatgtt ttcgcggaag agctcgcggt agatctcatc gcgggtcagg
     1561 ttgagctgtg ccttgaaggc ttcaaagtcc gatgtcatgg tcgcgcttgt ggtggcgggg
     1621 atcgtcgtgg cgcttggcgg atatgtgccg cctcagacgc ggaccagttt gccgtccatc
     1681 agcgctggca cgatggggca ggcatccaac gtggccgcgt agcggatgtc atcttccata
     1741 tcgcgcgcgg ccatgcgccg cccgaccttc gacgccatga ggatgtccag cgcgtcattt
     1801 tcgcggcaaa gccgcagtgc ggcgatggcc gagtcactca atgcgaagcg ccctgggtcg
     1861 ctccgttcga gcaggcggct gacgatgcag ccggcgccgt agaagtcctc cagcgaaaac
     1921 gtgccgcgag agcctgagca caccaacaac agggtctccg ttgtgtaatg cgccagcagg
     1981 tgatcggcca gcgcgctggc attcagaagc gagcctgcat acacctgttt gcaggccatg
     2041 gccccgtgca aggccactgt gccgttggtg gtcgcgtaga tgagatcctt tccagagagc
     2101 acggacgacc gcatggccag tgggttgtag ggatgaaagc cgggcagggt gtcggcatgg
     2161 tattcgcccg ccagtacgaa ttcccctggg ggaaagcctt gggataactc gcgtgcctgg
     2221 gcctcgtccc gtgccggcac gaccgaccgg gcgccttccc gcaaggcggt gacgatggtg
     2281 ctggtggcga agatgacgtc cagcaccacg gctatcttgc ctggcgccag ggctggatcc
     2341 agatcatctt tacggaagat cacgtgcact ttctgcatac tgcgtctcgg ttcggtcact
     2401 ccggcctgga gcgtgtttgg ggtgcgtggc ggactcgatg tttctcggtc tgcctctcga
     2461 cttgttcaga ggatccccag gtcaaccgcg cgcctgctca cgcagaatat tcttgcggat
     2521 tttacctgtg gacgtcttgg gcagcggacc gaacaccacg cttttcggca ccttgtacgc
     2581 cgccatgccg gcgcggcaga agtccaggat gtcctgctcg gtgatttctg gtgcatcttc
     2641 cttcaggcac acgaatgcgc agggcacttc gccccagtgg tcgtccggcc gtgccaccac
     2701 ggcggcttcg aaaatctgtg ggtgctgata gagcacatcc tcgacttcct tgctggaaat
     2761 attctcgcca cccgaaatga tgatgtcttt ggccctatcc ttgacctcga cgtagccgtc
     2821 gggatgcatg acggccagat cgcctgtgcg gaaccagccg ttgtggaacg cgcgctgcgt
     2881 gcttgccggg ttcttcagat agcctttcat caaggtgttg ccgcgatgca cgatctcgcc
     2941 cagcgtcttg ccgtccgcag gcaccggagt gtcgtcgtcc gccttcagga cctgcacatc
     3001 gtctatcgcg tacaagccta cgccctgacg cgccatcttg gcggccaggc tgtcgttgtc
     3061 caggttggcc cagtcgttct gccagacaca caccgttgac ggtccgtagc tttccgtcag
     3121 gccatacagg tgggtgatgg cgaagcccac tttgcccgcc cggccgatga cagcgctggg
     3181 tggcgccgcg ccgcccaatg cgaactggac tttttcgggt agtgaaacgc cttgtctgct
     3241 gtagtcatta accagcagat tgagcaccac gggcgcaccg cacatatgcg tgacgtgata
     3301 ctggcgtata cgttcgagca ccgcgtcgga ctgcaccttg cgcaagcaaa cctgcgtacc
     3361 gcccacagcg gcgatagccc aggtatagca ccatccgttg caatggaaca tgggcagcgt
     3421 ccacaagtag acggtgtccg gggtcatgtt gaaagccatg gcgttgctca tggcggacag
     3481 atacgcgccg cgatggtggt agaccacgcc cttggggtcc cccgtggtgc ccgaggtgta
     3541 gttcagggag atcgcgtccc attcgtcttc gggctttcgc cattcgaaat ccgggtcgcc
     3601 cgaggcaagc agggtttcgt agtcgtgcgc cgcgaaaccc ggcgacgcgg gcgcgacacg
     3661 cgcaatggcg acaaggatgg gtggggcatc catgccgcca accacctcct tgaccagggg
     3721 ctcgaattcc gtgtcgtaga ggagggcgcg agcctcgcca tgctggagga tgaagcgcag
     3781 cgcagcggcg tccagccgcg tgttgagcgc attcagcaca gcgcccgcca tgggcactga
     3841 ataatggctc tcgagcagtt cgtgactgtt gaaactcagg acagacaccg tgtccgcggg
     3901 ccggatgccc agcgacacca acgcgctggc catgcgccgg caccgttcgt aaagccgttc
     3961 gtaggtcagt gcgccgtcgt cgtccaccac ggcggtcttg tgaggaaata cccacgccga
     4021 tcgtttcagg aagctcaagg gcgtcaacgc cgaataattg gccgcctgtt tgtcgagacc
     4081 ggttcggtat ttattgtctg gcatggccat cgctcctgtt tggggtagtc aggaaaggta
     4141 tcgggggttg ttgatcgcca gtttcgcgcg tgtgagcgcc atgagcaata cggacagcgc
     4201 ctcgcggcgt ttcgacggga tgtcaccatt gcgcaggagc cgggccagcg tatgctcgga
     4261 aggtgtggta tcgatggcgt ccagatcggc ttcgcgcagg aaggccgcga tgcgcgcttc
     4321 gccgtcctgg tcggtggcgt cgcgctgttt cagttcgcgc gcggccactg ccatggcgcg
     4381 ggccaccatc ttggcttcgt aggttttatc ggaaggaagc agtgaggcca gctggttcag
     4441 caaaacatcg cgggcgacat ccagcaagtc ggcaccgctt ggcaagttgg tggtggcggt
     4501 cgctggaagg ctgcgggggt gtccgctgcc gaagccttcg tccgcacccg tgcggcaagc
     4561 catatccgtg tctgcgccgc gcgccgcttc catcccgcgg atctggctca agaggtcgaa
     4621 ctccatctcc ggcaccatgc gccccgtcaa cgccagctcc agcgacagct gttcgcctga
     4681 caagtggcgt tgcgcctgct gcagagcgat cacggcccag cgcacgaatc ccatgatttc
     4741 ccagtagcgc acgcgttcgg catcgacttc gtgccctgcc tggccggcgt aaccctcgaa
     4801 caggtcagct ttgtcgccaa ggccgccgac ttcgtggtca ttggcaccga atcgccagct
     4861 tttgcagcac agccagccca gatcctcgta cgggtcgctc caagtggcga attcccagtc
     4921 cagcacggcg gtcaagcggc catcgtcgac catgtagttg ccggtgcgaa agtcgcagtg
     4981 acacagggcc agattgcctg gcacgggagc atggtcacgc agccagtcca gcgcccattc
     5041 cagaaccgga tgcggctgcg gaatgtcagc cagtgccgcg cgataggtcc ccacgcgcca
     5101 aagggccgga ttgccggacg ggataggcag gaaccccaag tccgcccgag gagggtgaac
     5161 ctggtgaagc cgggcaagct ccaccccgag ccgtcgagtc agcgcccgag cctggtcatc
     5221 acccagcgca ccgcgcaaca gccggcgcgg cgacgcggaa ccggcgaccc gctccatcac
     5281 acagaagacg tgtccggcca cggattcgtc ctcacacaac cacaacggtc gcggcacggt
     5341 gacgcccgcc tcgtgggccg ccgccaatac atggaattcc tgggcacggg tcaggctggc
     5401 ggcgatgcgg gagggcgcgt cgctgcgcac gacgacgctc agccgacccg ggtgtgtgcc
     5461 accggtaagc atcaccgtca gcgcgtggtt gtcctggatg gcgccacccg ccaggcgtgc
     5521 gaactccaga acttcgacag catcggcacc c
//
LOCUS       DASTNK010000148        12215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1428371, whole genome shotgun sequence.
ACCESSION   DASTNK010000148 DASTNK010000000
VERSION     DASTNK010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12215
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428371"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /gene="fmt"
                     /locus_tag="VFR20_11260"
     CDS             complement(<1..835)
                     /gene="fmt"
                     /locus_tag="VFR20_11260"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU0231142.1"
                     /translation="MRIVFAGTPVFAQIALEAIIAAGYDVPLVLTQPDRPAGRGLKLL
                     PSPVKQAAQAAGIPVAQPRSLRLDGRYPDDATAAREALLRAEPDLMVVAAYGLILPSW
                     TLGLPAHGCFNIHASLLPRWRGAAPIQRAIEAGDAQTGITIMQMDEGLDTGDMLVSDP
                     VPISGDSTAASLHDALAEQGACSVLQAIAALRGGALAATPQPDEGVTYAAKLLKSEAP
                     LDFSQPAVVLERRVRAFNPVPGATMTLPGLADPVKVWMAQALDGPARQAPGGIESVSA
                     QG"
     gene            complement(896..1408)
                     /gene="def"
                     /locus_tag="VFR20_11265"
     CDS             complement(896..1408)
                     /gene="def"
                     /locus_tag="VFR20_11265"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU0231143.1"
                     /translation="MALLPILQYPDRRLHKVAKPVAQVDDRIRTLVRDMAETMYAAPG
                     VGLAATQVDVHERVVVIDVSEDGNDLHVLINPEIIWKSDEQQVYEEGCLSVPGVYDEV
                     ERSARIRARAQDEQGQIYEFDADGLLAVCVQHELDHLMGKVFVEYLSVLKQNRIRARL
                     RKQEREAQRA"
     gene            1585..2769
                     /gene="dprA"
                     /locus_tag="VFR20_11270"
     CDS             1585..2769
                     /gene="dprA"
                     /locus_tag="VFR20_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEU0231144.1"
                     /translation="MPSNLSDQELDAWIRLSLEPGVGSALARRLLGAIGLPPDIFALP
                     ASALAQCLPLDLARQLSLPPREEIVAAIEQTRVWLGEPDHHLLTLADAHYPAALLDTY
                     DPPPLLYVNGNPECLARPIISIVGARHASPGGEDNARAFARHLAERGWCIASGLALGI
                     DAAAHDGALLAGPDGGGTIAVMGTGIDIIYPARNHDLAHRIAQSGALVSELPLGTRAL
                     PHQFPKRNRIVAGLARGVLVVEAARQSGSLITARLATEAGREVFAIPGSIHSPLSHGC
                     HALIRQGAKLVESGQDIHDELGQARPASSPAVALAARTRLPPRQADPGPRTGNAKMLT
                     EATTAEQAAVLEALGHDPISLDVVHARTRLGIPQINQSLLELELAGRVDRLDDGRFQR
                     HE"
     gene            2813..3724
                     /locus_tag="VFR20_11275"
     CDS             2813..3724
                     /locus_tag="VFR20_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEU0231145.1"
                     /translation="MRFPESVKIVEVGPRDGLQNEKEFVPTEVKIELVDRLSNAGFQN
                     VESASFVSPKWVPQMADGAAVMAAIARRPGTIYSVLTPNLRGFHDAVQAGVDEVVIFG
                     AASEAFSQRNINCSIAESIARFEPVAEAAKRAGLRLRGSISCALDCPYQGPTPVSAVV
                     DVVQRMQALGCDEIDVADTIGTGTPGRVSDVFHAVGEVIDLKGVSGHFHDTYGQALAN
                     ILAALEAGVTIFHASVAGLGGCPYAKGATGNVATEDVLYLMHGLGIGTGIDLDAVVDI
                     GQWISGHLGRKAVSRAGNALAARKANA"
     gene            3721..4218
                     /locus_tag="VFR20_11280"
     CDS             3721..4218
                     /locus_tag="VFR20_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231146.1"
                     /translation="MTPPDTRLTPEEHAALISEIGPLPLRGMAWPTWVKVVVWIALIL
                     IGAQIIRVAGSPEGRQFSPLHAGFVILVFLALVVAARFMLFSETRITADGIEQSWFTL
                     RRIAWTDIQFARFVSLPTSKRLVCFTAGRRPVVFQAGTPELRNAFVRIAAAYRRRLEE
                     VRRHA"
     gene            complement(4286..5023)
                     /locus_tag="VFR20_11285"
     CDS             complement(4286..5023)
                     /locus_tag="VFR20_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU0231147.1"
                     /translation="MSAPDTPDSGVPSPSPTSMASRLDAIRDRMARACARAGRPASDV
                     ILLPVSKTFGVDAVREAQALGLHRFGENKTQEIAQKAPVVVDPTPEWVMIGHLQTNKA
                     KDAARLVAEVQSLDRLDLARALDRRLQHEGRAIDVLIQVKTSPEPSKFGLAPEELPGF
                     LKQLGHFDTLRVKGLMTMAVNANEEQPVRACFRMLRGWRDRLRDNGAEGLARLSMGMS
                     GDFELAIEEGSTEVRIGSALFGQRVYH"
     gene            complement(5188..6495)
                     /locus_tag="VFR20_11290"
     CDS             complement(5188..6495)
                     /locus_tag="VFR20_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU0231148.1"
                     /translation="MNAVNESWSSVADEGVLRFITAGSVDDGKSTLIGRLLVDSKGVF
                     ADQISAISRSRYKRVHDSEALDLALLTDGLEAEREQGITIDVAYRYFATPKRKFIVAD
                     APGHEQYTRNMVTGASTAQAAIILIDASRVVDGELLAQTKRHTALAALLGIQHIVVAV
                     NKMDLVGWNPELFEQIHVAYRDLAARLDIPHFHIVPLSALNGDNVVEPSANTPWYDGP
                     PLLSLLESLAVQDFNPRPPRFFVQWVVRDGPRGFRGYAGQLSGGSLAVGDTVRILPSG
                     VAAVIDRLVRFDQDLSVAQAGDTVTLVLDRDVDVSRGDLVVALEDTVRTGREFEADLC
                     WLDQRALSLQRSYLLKQGTRLTQARVRAVHTRRDIHELRHVQAGGALDMNDIGRVTIQ
                     ARDSLALDAYEDSPATGAFILIDEATHQTAAAGMIRQGGSDQV"
     gene            complement(6495..7403)
                     /gene="cysD"
                     /locus_tag="VFR20_11295"
     CDS             complement(6495..7403)
                     /gene="cysD"
                     /locus_tag="VFR20_11295"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEU0231149.1"
                     /translation="MGAPTPRTHLDWLESEAIYILREVAAECERPVLLFSGGKDSVVL
                     LRLAEKAFRPARFPFPLLHVDTGHNFDEVIAFRDAHVARIGERLIVRQVDDSIARGTV
                     VLRRENDSRNAAQATTLLEAIEEFGFDACIGGARRDEEKARAKERVFSFRNEFGQWDP
                     KAQRPELWNLFNTRVHKGENMRVFPISNWTELDVWQYITRERLALPPLYYAHQREVVR
                     RNGLLVPVTPLTPVRDGETAETLSVRFRTVGDVSCTCPVASTADTPEAIIAETVVSTM
                     TERGATRMDDQTSEASMERRKKQGYF"
     gene            complement(7405..8145)
                     /locus_tag="VFR20_11300"
     CDS             complement(7405..8145)
                     /locus_tag="VFR20_11300"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEU0231150.1"
                     /translation="MNVSESFSSVTDSAVRRLWVGLLERLAGISARHPDAAFASSLGA
                     EDMLLSHAIFHLGLPIHVVTLDTGRLHPETLAMIETVRERYGVALDVLRPDSVRVSEH
                     VTRHGTHAFYESLALRKECCHIRKVEPLARALRGRSAWLTGQRQGQGATRAQLVEGEF
                     DPVFGLFKYNPLFDWTGEQVWTALRALDVPYNPLHDQGYPSIGCEPCTRAVRTDEDER
                     AGRWWWEQSSSRECGLHAGNLQMKNTRG"
     gene            complement(8304..9197)
                     /locus_tag="VFR20_11305"
     CDS             complement(8304..9197)
                     /locus_tag="VFR20_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0231151.1"
                     /translation="MGSRISTARGAGTWWRLGAPAVFLLFWSSGFAIGKVGLQYAPPL
                     ILLSVRYACVVVILLPLQWIFRTRLPRTARQWFDLCVVGFLIQFVYFSMSYMGMKAGL
                     SAGGIALIVSMEPILVGIVSPATVGERVPWMQWLGLALGLAGAVLVIAAKSRVELASP
                     LGVGLAVCALIGMTAGVLYEKRYSQQVHPIGANLAQCTVGLVFCLPLALATETTHIDW
                     TWQFSGALAYLVVCNSLISISLLLAMVRRQQAARVSALFFLVPPGAALMGMLLLGESM
                     PFAAWVGMAIAAAGVWIANRT"
     gene            9564..10853
                     /locus_tag="VFR20_11310"
     CDS             9564..10853
                     /locus_tag="VFR20_11310"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEU0231152.1"
                     /translation="MSEHRAHTLPSYLNPRQLGPWGTYLEQVDRVTPYLGRLGKWVET
                     LKRPKRALIVDVPVELDNGTIAHFEGYRVQHNTSRGPGKGGVRFHQDVTLSEVMALSA
                     WMSVKSAAINLPFGGAKGGVRIDPHNYSRAELERITRRYTSEIGAIIGPNKDIPAPDV
                     NTNAQTMAWMMDTYSMNEGATTTGVVTGKPVSLGGSLGRVEATGRGVFVVGCEAANDA
                     GIKIKGARVVVQGFGNVGGTAARLFYEAGAKVIAVQDHTGCVHNAKGLNVLTLLEHVE
                     KNGGVAGAVDADNITATEFWALETEFLIPAALEGQLNKHNADSIRARIVIEGANGPTT
                     PEADDILSAKGTVIVPDVVANAGGVTVSYFEWVQDFSSFYWTEVEINHRLEAMMKAAY
                     AATSAVAKEHNVSLRTAAFIVACTRILEAREVRGLYP"
     gene            complement(10956..11423)
                     /locus_tag="VFR20_11315"
     CDS             complement(10956..11423)
                     /locus_tag="VFR20_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEU0231153.1"
                     /translation="MDRKDLEILKVLQEDATVSLAELAEAVNLSSTPCWRRVQKLREE
                     GVIRRQVVLCDPGKLNVGLSTFVLVRTSHHDDAWMRRFVEAANGIPEIVEIYRMSGDI
                     DYLLKIVVPDMAGYDGVYKRLIAAVELLDVSATFAMEVIKSSTALPLDYALRD"
     gene            complement(11541..>12215)
                     /locus_tag="VFR20_11320"
     CDS             complement(11541..>12215)
                     /locus_tag="VFR20_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU0231154.1"
                     /translation="VPFEVRVLYRDARLVVVDKPHFLASVPSGRYLRETALVRLRRDL
                     DLPTLSPLHRLDRETAGVMLFCVDPACRGAYQALFQARQVDKVYEAVAPVVDGLTFPL
                     VRRSRLRACTGRFTMEEVEGAPNSETHIQLTARCAGGLGLYRLRPATGRKHQLRAHMS
                     ALGMPILHDTLYPETHRPAVENYERPLQLLARAIRFRDPFSGRVRCFQSERWLDADAS
                     RQGDGC"
BASE COUNT         2318 a         4177 c         3687 g         2033 t
ORIGIN      
        1 cgccctgggc cgacactgac tcaatgccgc cgggggcttg ccgggccggg ccatccagcg
       61 cctgcgccat ccagaccttc actgggtcgg ccaggcccgg cagcgtcatg gtggcacccg
      121 gcacggggtt gaatgcccgt acgcggcgtt ccagtacgac agctggttgg gaaaaatcca
      181 gaggcgcttc ggatttgagc agtttggcgg catacgtgac accctcatcg ggctggggcg
      241 tggcggccag tgcgccgccc cgcagcgccg caatggcctg caacacgctg cacgcgccct
      301 gctcggccag cgcatcgtgc aagctggccg ccgtcgagtc gccagagatc ggcacggggt
      361 ccgacaccag catatcgccc gtatccaacc cttcgtccat ctgcatgatg gtgatacccg
      421 tctgcgcgtc acccgcctcg atggctcgct ggatgggggc cgccccgcgc cagcggggca
      481 acaggctggc atgtatgttg aagcagccat gcgctggcag ccccaacgtc caggaaggca
      541 agatcaggcc ataggccgcc accaccatga ggtcaggctc cgcgcgcagc aacgcctcgc
      601 gcgccgccgt ggcgtcatcg ggatagcgac cgtcgagtcg caggctgcgc ggctgcgcca
      661 cgggaatgcc ggcggcctgg gccgcctgct tgacgggaga aggcaacagt ttcaatcccc
      721 gcccagccgg gcggtcgggt tgtgtcaaca ccaggggaac gtcatacccc gcagcgataa
      781 tggcctcaag ggcaatctgt gcgaacaccg gggtgccggc gaaaacgatg cgcatcaagc
      841 gtgccttgga gtgctgtttg gtgaatttga atgcatgggc ccgtcaagcc gggccctagg
      901 cgcgctgcgc ttcccgctct tgcttgcgca ggcgggcacg gatccggttc tgcttgagca
      961 ccgacaggta ttccacgaac accttgccca tgagatggtc gagctcgtgc tggacacaaa
     1021 ccgccagcag gccgtcagcg tcgaactcgt atatctgtcc ctgttcgtcc tgcgcccgcg
     1081 cgcgaatgcg ggcggaccgc tcgacctcgt cgtaaacacc cggcaccgac agacaacctt
     1141 cttcgtacac ctgctgctcg tcgcttttcc agatgatttc cgggttgatc agcacatgca
     1201 ggtcgttgcc gtcctcggag acatcgatga cgaccacacg ctcgtgtacg tcgacctggg
     1261 tcgccgccag ccccaccccg ggcgcggcgt acatggtttc cgccatgtcg cgcaccagtg
     1321 tgcggatgcg gtcatcgacc tgggcaacgg gcttggcgac tttatgcaga cgccgatccg
     1381 gatattgaag aatgggtagc aaagccataa taaggcacat ccaaaatgag tgctttcaac
     1441 cctctatcat agaacatcca cgaccgttgg tcaggttcaa gccagcgaaa tccaagcccg
     1501 cgaaagcggg acctttccgc gccgggggca ccggcaacgc atcacgccat gcgacactaa
     1561 ggaaattcgt caattcgcat tcctatgcct tccaacctgt ccgaccagga actcgacgcc
     1621 tggatacgcc tctcgctgga acccggcgtg gggtccgctc tggcgcgccg cctgctgggc
     1681 gccatcggcc tgccgcccga cattttcgcg ctgcccgcca gcgcgctggc gcagtgcctg
     1741 cccctcgatc tcgctcgcca actcagtctt ccgcctcgcg aagagatcgt cgcggccatc
     1801 gaacaaaccc gggtctggct tggggagcct gatcatcacc tgctcacact ggcggacgcc
     1861 cactaccccg ccgccctgct cgacacttac gatccaccgc cgctgctcta cgtgaacggg
     1921 aaccccgagt gcctcgcacg ccccatcatt tccattgttg gcgcccgtca cgcctcaccc
     1981 ggcggcgagg acaacgcccg cgcgttcgcc cgccatctcg ctgagcgcgg atggtgcatc
     2041 gccagcggcc tggccctggg catagacgcc gccgcgcacg acggcgcgct gctcgccggc
     2101 cccgacggcg gcggcacgat cgccgtcatg ggcacgggca tagacatcat ctatccggcc
     2161 cggaatcacg acctggcgca tcggatcgca caaagcggcg ccctggtttc cgagcttccg
     2221 ttgggcacac gcgcgctgcc gcaccaattc cccaagcgca accgcatcgt cgcgggcctt
     2281 gcccgggggg tgctggtggt ggaagccgcc cggcaaagcg gctctctcat cacggcccgg
     2341 ttggccacgg aggcgggccg tgaagtgttc gcgatccccg gttccatcca ctcgcccttg
     2401 tcccacggat gccatgccct gatacgccag ggagccaagc tggtcgaatc aggccaggat
     2461 atccacgacg aactcggcca ggcgcgcccg gcttcctcgc ccgccgtggc gctggcggcc
     2521 cgcacccgcc tcccgcctcg tcaagccgac cccggccccc ggaccggaaa cgctaaaatg
     2581 ctgaccgaag cgaccacagc cgaacaagcg gccgtgctcg aagccctggg acacgatcca
     2641 atcagtctcg acgtcgtgca tgcccgcacc cggctcggca ttccacaaat caaccagagc
     2701 ctgctcgaac ttgaactggc cgggcgcgtc gaccggctcg acgacggccg tttccaacgc
     2761 cacgaatgaa cgccacatgg cgcccgcaca ccacgtaccg aggatacaca ccatgcgttt
     2821 cccagaaagt gtcaagatcg tcgaggtcgg cccgcgcgac gggttgcaga acgaaaagga
     2881 attcgttccc accgaggtca agatcgaact cgtcgaccgc ttgtcgaacg ccggcttcca
     2941 gaacgtcgaa tcggcatcgt tcgtgtcgcc gaaatgggtg ccgcaaatgg cggatggcgc
     3001 cgccgtcatg gcagcgatcg cgcgcaggcc cggcaccatc tactcggtgc tcacgcccaa
     3061 tctgcggggc tttcacgatg ccgtccaggc cggtgtcgac gaggtggtga ttttcggagc
     3121 cgccagcgag gcattctccc agcgcaacat caactgctcg atcgcggaat ccatcgcgcg
     3181 cttcgaaccc gtggcggagg ccgcgaaacg cgccgggctg cgtctgcgcg gcagcatcag
     3241 ctgcgcgctg gactgcccct accagggccc gacacctgtg tcggcggtgg tggacgtcgt
     3301 ccaacgcatg caggcgctgg gctgcgacga aatcgacgtg gccgacacca taggcaccgg
     3361 cacacccggc cgcgtgagcg acgttttcca tgcggtgggg gaggtcatcg acctgaaagg
     3421 cgtgtccgga catttccatg acacctatgg ccaggcgttg gcgaacatcc tggcggcgct
     3481 tgaagccggc gtcacgatct tccacgcctc ggtggctggc ctgggcggat gcccctacgc
     3541 gaaaggcgcc accggcaacg tggccacgga agacgtgctc tacctcatgc acggactggg
     3601 aatcggcacc ggcatcgatc tcgacgccgt ggtggacatc ggccaatgga tatcggggca
     3661 cctgggccgc aaggcggtca gccgggcggg caacgccctg gccgcgcgca aggccaatgc
     3721 atgacaccgc cggacacccg gctcacaccc gaggagcacg ccgccctgat cagcgagatc
     3781 gggccgttgc cgctgcgcgg catggcgtgg cccacctggg tcaaggtggt tgtgtggatc
     3841 gccctgatcc tgatcggcgc ccagatcatc cgtgtcgcgg gcagtccgga aggccggcag
     3901 ttcagcccgc tgcatgcggg tttcgtcatt ctcgtgtttc tggcgctggt cgtggccgca
     3961 cgcttcatgc tgttctcgga aacccgcatc accgcggacg gtattgagca atcctggttc
     4021 acgctgcgcc gcatcgcctg gacggacatc cagttcgccc ggttcgtttc gctgcccacc
     4081 tcgaagcgat tggtgtgctt caccgctggg cgcaggcctg tggtcttcca ggccggcacg
     4141 cctgagctac ggaacgcttt cgtgcgcatc gcggcggcct atcgtcgtcg actcgaagag
     4201 gtgcggcgcc acgcatgagg ctgtggcgcg catcccgggg ggcggaaagc acaagcccgc
     4261 ccgccaggcg atacgcctgc atgcctcaat ggtagacacg ttgcccgaaa agcgccgacc
     4321 cgatgcgcac ctcggtcgac ccttcctcga tggccagttc gaaatccccg ctcatgccca
     4381 tggataaacg cgccaggcct tcggcgccat tgtcgcgcag gcggtcgcgc cagccgcgca
     4441 gcatgcgaaa acacgcccgg acaggctgtt cctcgttggc gttgacggcc atggtcatca
     4501 accccttgac gcgcagcgta tcgaaatgcc cgagttgttt gagaaacccc ggcaattcct
     4561 ccggcgccag cccgaatttc gagggttccg gcgaggtctt gacctgaatc agcacatcga
     4621 tggcgcggcc ctcgtgctga aggcgccgat ccagcgcccg ggccaggtcc aggcgatcaa
     4681 gcgactgcac ctcagcgacc aggcgcgcgg cgtccttggc cttgttcgtc tgcagatggc
     4741 cgatcatcac ccattcaggg gtggggtcga ccaccacggg ggccttttgc gcgatctcct
     4801 gcgtcttgtt ctcaccgaat ctatgcagcc ccagcgcttg cgcctcgcgc accgcgtcca
     4861 cgccgaaggt cttgctgacg ggcagcagga tgacatcgga tgcagggcgg cccgctcggg
     4921 cacacgcccg ggccatgcga tcgcgtatcg catcgagccg ggatgccatg gaggtcgggg
     4981 acggggaggg aacgccggaa tcaggggtat cgggggctga catgaaactc ggttgtggca
     5041 cgaaagagga atgacgcctc taccatacaa caagaaccgt ttcgcgcgcc ggaccccgcc
     5101 cacacaggta gtccaacccc cggtgcggcc cgttccgcac gggagtcctt agtagtcctc
     5161 gaaaccagac accgcactcg ctttccgcta gacctggtca gaaccccctt gacgaatcat
     5221 cccggccgcg gcggtctggt gcgtggcctc gtcgatcagg atgaacgccc ccgtggccgg
     5281 gctgtcttcg tacgcatcca gcgccaggct atcgcgggcc tggatggtca cccgaccgat
     5341 gtcgttcata tccagtgcgc cgcccgcctg cacgtgccgc agctcatgga tgtcgcgccg
     5401 ggtatgcacc gcgcggacac gggcctgcgt caggcgggtg ccctgcttca ggagatacga
     5461 tcgttgcagg ctcagcgccc gctgatcgag ccagcacagg tcggcctcga actcgcgtcc
     5521 ggtgcgcacc gtgtcctcca aggccaccac caggtcgccg cgcgagacgt cgacgtcccg
     5581 atcgagcacc agtgtcaccg tatcgccggc ctgcgcgacg ctcaggtcct ggtcgaaccg
     5641 caccaagcga tcgatcacgg ccgccacgcc cgaaggcaat atcctcaccg tatcgcccac
     5701 cgccagcgaa ccgccggaca attgacccgc gtagccccgg aaccctcgag gcccatcacg
     5761 cactacccat tgcacgaaga accgcggcgg cctgggattg aaatcctgga cagccaggga
     5821 ctccagcagc gacagcagcg ggggaccgtc ataccaaggc gtattggccg atggctcgac
     5881 cacgttgtcg ccattgagtg ccgacagggg gacgatatga aagtgcggaa tgtccagcct
     5941 ggccgccaga tcccgatagg cgacatggat ctgttcgaag agctccggat tccacccgac
     6001 cagatccatc ttgttcaccg ccacgacaat gtgctggatg cccagcagcg ccgccaacgc
     6061 ggtatgccgt ttggtctgcg ccagcagctc gccatcgacg acccggctcg cgtcgatcag
     6121 gatgatcgcg gcttgcgcgg tcgacgcgcc cgtcaccatg ttgcgggtgt actgttcgtg
     6181 gcccggcgca tccgcgacaa tgaatttgcg cttcggcgtg gcgaaatacc gatacgccac
     6241 atcgatggtg atgccctgct cgcgctccgc ctccaggccg tcggtgagca gcgccagatc
     6301 cagggcttcg ctgtcgtgca cccgtttgta ccgggaacgg gaaatggccg atatctggtc
     6361 ggcgaacacg cctttgctgt caaccaacag gcggccgatc agcgtggatt ttccgtcatc
     6421 caccgagccg gccgtgatga agcgcaacac gccttcgtcc gccacactgc tccaggattc
     6481 attgactgcg ttcatcagaa gtacccctgt ttcttgcggc gctccatcga ggcctcggaa
     6541 gtctggtcat ccatgcgtgt ggcaccgcgc tcggtcatgg tggacaccac cgtttcagcg
     6601 atgatcgctt cgggggtgtc ggccgtggac gccaccggac aggtgcatga cacgtctccg
     6661 accgtgcgaa accgcacgct cagcgtttca gccgtttccc cgtcccgcac cggcgtcaga
     6721 ggcgtgaccg ggacgagcag gccgttgcga cgcaccacct cgcgctgatg cgcgtaatag
     6781 agagggggta atgccagccg ttcccgcgtg atgtactgcc agacgtccag ctcggtccag
     6841 ttcgagatgg ggaagacccg catgttctcg cccttgtgca cacgggtgtt gaacagattc
     6901 cacaactccg ggcgctgagc cttcgggtcc cactgcccga actcgttgcg gaacgagaac
     6961 acacgttcct tggcccgggc cttttcctca tcacgccgcg ccccgcctat gcaggcatcg
     7021 aaaccgaact cctcgattgc ctcgagcagc gtggtggcct gcgcggcgtt gcgtgaatcg
     7081 ttctcgcggc gcaggaccac cgtgccgcgc gcgatcgagt cgtcgacctg gcgcacgatc
     7141 agtcgctcgc cgatgcgggc gacatgcgcg tcgcggaatg cgatcacttc gtcgaagttg
     7201 tggccagtat cgacatgcag cagcggaaac ggaaagcgcg cgggacggaa ggccttttcc
     7261 gccaggcgca gcagtacgac cgaatccttg ccaccggaaa acaacagcac cgggcgttcg
     7321 cactcggccg cgacttcccg caatatgtag atggcttcgg attcgagcca gtccagatgt
     7381 gttcttggtg tgggtgcacc catttcaacc tctcgtgttc ttcatttgta gattccccgc
     7441 gtggagcccg cactcacggg aggaggactg ctcccaccac cagcgccccg cgcgttcgtc
     7501 ctcgtccgtc cgcacggcgc gcgtacacgg ctcgcagcct atcgacggat acccttggtc
     7561 gtgcaacggg ttgtagggaa cgtccagcgc acgcaaagcc gtccatacct gttcccccgt
     7621 ccaatcgaac agtgggttgt acttgaacag cccgaacaca gggtcgaact caccctctac
     7681 cagctgcgcg cgggtggcgc cctgcccctg gcgctgcccc gtgagccacg ccgagcgtcc
     7741 ccgcagcgcg cgcgccaggg gctccacctt gcggatatga cagcattcct tgcgcaaggc
     7801 caggctttca tagaaggcgt gtgtgccatg gcgcgtgacg tgctccgaca cccgcacaga
     7861 gtcgggccgc agcacgtcca gcgccacgcc gtagcgttcc cgtacggtct cgatcattgc
     7921 caatgtttcg gggtgcagcc gaccggtgtc caacgtgacc acgtggatcg gcaaccccag
     7981 atggaaaatg gcgtgcgaca acagcatgtc ttccgctccc agcgacgacg caaaggccgc
     8041 atcggggtgg cgggccgata tcccggccaa acgctcgagc aagccgaccc atagccgccg
     8101 cacggcggaa tcggtaacgg aagaaaagga ctcggaaacg ttcatcgcgg cgcctgttga
     8161 ttggtcaacc ggcgttatgt cgaactggct cacgcgttgc ccgacaggac gccaggacac
     8221 agtctgccgg gcgcggagcg gcttgggaac gatttaatcg ttaagtgttt atggctgtca
     8281 gccataaggt ggacgccgcg gtgtcacgtc cggttcgcga tccagacacc cgccgcagcg
     8341 atcgccatgc ccacccatgc cgcgaacggc atgctctcgc ccagcagcag catccccatc
     8401 aaggcggcgc cgggaggcac gagaaagaac aacgccgaca cccgcgcggc ctgctgacgc
     8461 cgcaccatgg ccagcagcaa tgaaatggaa atcagcgagt tgcacacgac cagatacgcg
     8521 agtgcgccgg agaactgcca ggtccaatcg atgtgggtcg tctcggtcgc cagcgccaaa
     8581 ggcaggcaga acaccaagcc cacggtacac tgcgccagat tggcaccaat cgggtgaacc
     8641 tgttggctat agcgcttttc gtaaaggacg ccggcggtca tgccgatcag cgcgcagacc
     8701 gccagcccca cacccagggg cgaggccagt tcgacccgtg acttggctgc gatgacaagc
     8761 acggcgccag ccaggcccaa cgccagaccc agccattgca tccatggcac cctttccccc
     8821 accgtcgcgg gcgacacgat gcccaccagt atgggctcca tggacacaat gagcgcgata
     8881 ccgcccgcgg acagtcctgc tttcatgccc atgtacgaca tactgaaata cacgaactga
     8941 atcaggaaac cgaccacaca caggtcgaac cattggcggg cggtgcgcgg cagtcgtgtg
     9001 cggaagatcc actgcagggg cagcaggatc accaccacgc aggcatagcg caccgacaga
     9061 aggatcagtg gcggcgcgta ctgcaacccg accttgccaa tggcgaaccc ggacgaccag
     9121 aacaatagaa acaccgccgg ggcgccgagc cgccaccacg tgccagcccc gcgcgctgtg
     9181 gaaatccggg aacccatggg tatgtgcctg atccaactcg atgatggcga cgggaacaac
     9241 cgccgacagg cgcaatcata aagcatagag gactgtggcc gggaacacca tcgggatcat
     9301 tccggtcgcg gcccgcgccg cacggcgcgc agaatagaca gcggaacgcc ggtgtcgcgc
     9361 cccacaagtt cgcggacagc gtctttcagt attaacacgg caaaatgttg tggatgcgcc
     9421 aacggcatcc actgccgtat aatgcgtccc taatttaatt tcattgaaat aaattaggga
     9481 catagtaagc taggcactcc cgaaagcccc attccctggg cttccgcaac gattcctctc
     9541 tcaacgctca aggttacgtc accatgtcag aacaccgtgc gcacacgctt ccttcctatc
     9601 tgaacccccg gcaattgggc ccatggggta cctatctcga acaagtcgat cgcgtcacgc
     9661 cgtacctggg caggctgggc aaatgggtcg agaccctcaa gcggcccaaa cgcgcgctca
     9721 ttgtggatgt acccgtcgag ctcgacaacg gcaccatcgc gcacttcgaa ggctatcgtg
     9781 tccagcacaa caccagccgc ggtcccggca agggcggagt gcgcttccat caagacgtca
     9841 cactatccga agtcatggcg ctgtccgcct ggatgtctgt gaaatcggcc gccatcaacc
     9901 tgccgttcgg cggcgccaaa ggcggcgtcc gcatcgaccc gcacaattac tcccgcgccg
     9961 aactcgaacg catcacgcgc cgctacacca gtgaaatcgg tgccatcatc ggccccaaca
    10021 aggacatccc cgcccccgac gtgaacacca atgcccagac catggcctgg atgatggaca
    10081 cgtactccat gaacgaaggc gccaccacaa cgggtgtggt cacaggcaag ccggtgtctc
    10141 tgggcggtag cctgggacgc gtcgaggcca ccggccgcgg cgtgttcgtc gtggggtgcg
    10201 aagccgccaa cgacgccggc atcaaaatca aaggcgcacg agtggtcgtg cagggtttcg
    10261 gcaatgtggg cggcacggcg gcgcgcctgt tctacgaagc cggtgccaaa gtgatcgcgg
    10321 tgcaggacca cacgggttgc gtgcacaacg ccaaggggct caacgttctc acactactcg
    10381 aacatgtcga gaagaacggc ggtgtggccg gcgcggttga tgccgacaac atcaccgcca
    10441 ccgaattctg ggcgctggaa accgaatttc tgatccccgc cgccctggaa ggccaactca
    10501 acaagcacaa cgccgactcc atccgcgccc gcatcgtgat cgaaggcgcc aacggcccca
    10561 ccacccccga agccgatgac attctcagcg ccaagggcac tgtcatcgtg cccgacgtgg
    10621 tggccaacgc tggcggcgta acggtcagtt atttcgagtg ggtgcaggac ttctccagct
    10681 tctactggac tgaagtcgag atcaaccacc ggctcgaagc catgatgaag gcggcctatg
    10741 ccgcgacctc ggccgtggca aaggagcaca acgtgtcgct gcgcaccgcc gcgttcatcg
    10801 tggcctgcac ccgcatcctg gaggcacgtg aggtgcgcgg gttgtatccc tgattcagct
    10861 caggcatccg catcgacccg cccgtccccg atcctgtccg gcacaccgcg cccctttggc
    10921 gggcggcgcg ccgaacagga tactgtggac gaccatcaat cgcgcagcgc gtaatccagc
    10981 ggcagcgctg tcgacgactt gatgacttcc atggcgaagg tggcgctgac gtccagcaat
    11041 tccacggcgg cgatcaagcg cttgtatacc ccgtcgtagc ccgccatgtc gggcacgacg
    11101 atcttcagca ggtaatcgat gtcgccgctc atgcgataga tctcgacgat ctctggaata
    11161 ccgttcgccg cttcgacgaa acgccgcatc catgcgtcat cgtggtggct ggtgcgcacc
    11221 agcacgaacg tcgacaagcc cacgttgagt ttgccggggt cgcacagcac cacttgccgg
    11281 cgtatcaccc cctcttcgcg cagcttctgt acccggcgcc agcatggcgt cgacgacaga
    11341 ttcaccgctt cggccagctc ggccagcgac acggtggcgt cctcttgcaa aactttcagt
    11401 atttccagat ccttgcgatc catggtcgac cttgtccccg ttcctctgct cactccgccg
    11461 gattatgcct catgcacgga aacccgggcc ggtttcccat cgacccgccg gagccgggcg
    11521 gtctcgcgtg tgaacaggca ctaacaccca tccccctggc gggaggcgtc ggcatccagc
    11581 catcgctcac tctggaagca ccgaacccga cccgagaaag gatcccggaa ccggatcgcg
    11641 cgagccagta gctgcaacgg ccgttcgtaa ttctcgaccg ccgggcgatg cgtctccgga
    11701 taaagcgtgt cgtgaagaat gggcataccc agcgcgctca tgtgagcgcg caactgatgc
    11761 ttgcggcccg tggcggggcg caagcggtat agaccgagac cacccgcgca gcgcgccgtc
    11821 aactgaatat gcgtctcgct attcggggcg ccttcgactt cttccatcgt gaaacggccc
    11881 gtgcacgccc gcaggcggct gcgccgcacg agcggaaacg tcaaaccgtc gacgacaggc
    11941 gccaccgcct catagacctt gtccacttgt cgtgcctgga acaacgcctg gtatgcgcct
    12001 cggcaggccg ggtccacgca gaacagcatg acccccgccg tctcgcgatc cagccgatgc
    12061 aacgggctga gagtgggaag atccagatca cgacggagac gcaccagcgc cgtttccctc
    12121 agataacggc cgctgggcac actggccagg aagtggggct tgtccacgac gaccaggcgg
    12181 gcatcccgat acaacacgcg aacctcgaac ggcac
//
LOCUS       DASTNK010000149        54004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_876300, whole genome shotgun sequence.
ACCESSION   DASTNK010000149 DASTNK010000000
VERSION     DASTNK010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 54004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54004
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876300"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VFR20_11325"
     CDS             complement(<1..257)
                     /locus_tag="VFR20_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-(5'-phosphoribosyl)anthranilate isomerase"
                     /protein_id="HEU0231155.1"
                     /translation="MTISRTRVKICGMTRANDVRTAVDCGADAIGLVFYPKSGRVVSV
                     EQARALRQTVPAFVSAVALFVDPTVEEVNRVIDQVRPDLLQ"
     gene            complement(289..1125)
                     /gene="truA"
                     /locus_tag="VFR20_11330"
     CDS             complement(289..1125)
                     /gene="truA"
                     /locus_tag="VFR20_11330"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU0231156.1"
                     /translation="MPRMALGVEYDGSAWNGWQSQPHSRTVQDVLDDALSRFLDEPVT
                     TVCAGRTDTGVHALGQVAHVDASASRRLESWVRGTNALLPPSIAVRWAQPVPDTFHAR
                     FSAVSRTYIYVVRNARVRSPLLHARVGWVHQPLDLAHMRAAASPLAGEHDFSAFRSSQ
                     CQAHSPVRNLRRLDIAQRDEFFFFRFTANGFLHHMVRNLVGALLYVGQGREAPDWVRL
                     LLQGRDRRLAAPTFSAAGLYFMGVEYPSGFELASLDAARDEPGLPGGRPPWDVACFDI
                     LD"
     gene            complement(1132..3057)
                     /locus_tag="VFR20_11335"
     CDS             complement(1132..3057)
                     /locus_tag="VFR20_11335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEU0231157.1"
                     /translation="MSAPACAARLGHSRIVSAPGQPLKIDVPLVGLDAVEQATLRVWL
                     AEPDAWRQAGLNPPVSLDSMHVRVTYGSGRRSRLIEISSNQIFDGSVADLLLGVSSAE
                     GEQRYQISILSDARGAGAPVLAAGPRLGSQTHGVTTHHAQAIRVRRGDTMFAIARRHA
                     VPGVTVYQMMIALQRANPRAFIRHNLNLVKAGAALAMPDRAALTAVSDREARRLFMQQ
                     VREFNAYRQRLAGESTPPVVGASAAAGRVSQEAQAPKPAPVTPQDRVVLSSGASSTDR
                     AADQKASTGKAVADSRQRITELEQNIHHLNQALQGSGSGASGAGTAATANGSAPSSAS
                     NASGAGTGGGSSAGGVTAPMLANAARGTASSSGAAGSSDSTGHGASNADVGTVTSAGV
                     VGGSAASGGQGTPQTATTKMAAAGHPEGAGGVAPSHSGNAAVAQSATQAGHTTIAQSS
                     AAEAGGSVSGSTEGIMAGSSGSAASHGTAGTADVNASGASGGGTGSQAGEVPGKTNPA
                     GKAAASGASGAVTAGNSTSSSEASSAPSAPSAPNEASTGGASNTHAAGAGESAASNKS
                     GKNVSWIQEHMLGVVTALLALIVLIIAWVLRRINTVRDDGGNGPRITEAMVQEKLDQI
                     NLDLRQSSGDGPAPAKD"
     gene            complement(3146..4276)
                     /gene="asd"
                     /locus_tag="VFR20_11340"
     CDS             complement(3146..4276)
                     /gene="asd"
                     /locus_tag="VFR20_11340"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU0231158.1"
                     /translation="MTQAVGLVGWRGMVGSVLMQRMRDENDFSLFEPVFFSTSNAGGA
                     APDWAKGAAPLQDAHDIAALKKLPIIVTAQGGDYTTAVYSKLRAAGWDGIWIDAASTL
                     RMADDAIIVLDPVNRPVIDTAIGRGVRNFIGGNCTVSCMLMGLAGLFNNDLVEWMTSM
                     TYQAASGGGAQHMRELLVQFGLLNNAVGGLLDDPASAILAIDRGVLARQQAADLPCDQ
                     FGVPLAGSLIPWIDKDLGNGVSREEWKAEAETNKILGRGPAFGTAPTPIDGLCVRIGA
                     MRCHSQALTIKLKKDVGLDEISDMLREGSRWARIVPNEREVTMRELTPVAVTGTLDIP
                     VGRLRKMSMGSDYLSAFTVGDQLLWGAAEPLRRMLRIILGEL"
     gene            complement(4367..5443)
                     /gene="leuB"
                     /locus_tag="VFR20_11345"
     CDS             complement(4367..5443)
                     /gene="leuB"
                     /locus_tag="VFR20_11345"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU0231159.1"
                     /translation="MTHKIAVLPGDGIGTEITEQAVRVLTALGLDLELESAPVGGAAY
                     DAAGHPLPPATLELAKRSDAVLFGAVGDWKYDHLARELRPEQAILGLRKALGLFANLR
                     PAILYPELANASSLRPEVVSGLDILIVRELTGDIYFGQPRGVRKAPEGPFAGELQGFD
                     TMHYAESEVRRIAHVGFQAASKRRGKLCSVDKSNVLETSQFWRDIVIDVSQEYPDVEL
                     SHMYVDNAAMQLVRAPKEFDVIVTGNLFGDILSDEAAMLTGSIGMLPSASLNASNQGL
                     YEPSHGSAPDIAGKNVANPLATILSAAMLLRYSLNAGAVAERIEQAVRAVLRQGLRTA
                     DIFEEGTTRVSTSQMGDAVLHALK"
     gene            complement(5621..6271)
                     /gene="leuD"
                     /locus_tag="VFR20_11350"
     CDS             complement(5621..6271)
                     /gene="leuD"
                     /locus_tag="VFR20_11350"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU0231160.1"
                     /translation="MQAFTTHEGIVAPLDRANVDTDLIIPKQFLKSIKRTGFGPNLFD
                     ELRYLDHGEPGMDNSRRPLNPDFVLNQPRYQGASVLLARKNFGCGSSREHAPWALAQY
                     GFRAIIAPSYADIFFNNSFKNGLLPIVLSELEVSRLFDEVRAFSGYTLRIDLPEQAII
                     TPEGRKLGFDIEPFRKHCLVNGLDDIALTLRKADLIRAFESEHLARHPWLEGRPVA"
     gene            complement(6281..7684)
                     /gene="leuC"
                     /locus_tag="VFR20_11355"
     CDS             complement(6281..7684)
                     /gene="leuC"
                     /locus_tag="VFR20_11355"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEU0231161.1"
                     /translation="MAQTLYDKLWNAHIVHQESDGTCLLYIDRHLVHEVTSPQAFEGL
                     ALAGRKPWRVSANLAVSDHNVPTTARDQGIQDPVSRLQVDTLDSNCEKYGVTQFRMND
                     RRQGIVHIIGPEQGATLPGMTVVCGDSHTSTHGALGTLAFGIGTSEVEHVLATQTLLM
                     KKSRSMQINVEGELPFGCTAKDLVLYIIGQIGTAGGTGCAIEFSGATIRALSVEGRMT
                     VCNMAIEAGARSGMVAVDDKTIEYFRGRPYAPTGVLWDQAVKYWRTLHSDPGATFDRV
                     VQIDARKVAPQVTWGTSPEMVLAVDARVPDPDKEKDDVKRGGMERALEYMGLKPNTPL
                     TDIKIDRVFIGSCTNARIEDLRAAAQVARGKRVAANVRQAMVVPGSGLVKAQAEKEGL
                     DKIFLGAGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGQGGRTHLVSPAM
                     AAAAAIAGHFVDVRNFR"
     gene            complement(7835..8896)
                     /gene="aroC"
                     /locus_tag="VFR20_11360"
     CDS             complement(7835..8896)
                     /gene="aroC"
                     /locus_tag="VFR20_11360"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU0231162.1"
                     /translation="MSGNTLGKLFCVTNFGESHGPAIGAVVDGCPPGLPLSETDIQKE
                     LDRRRPGTSRHVTQRQEADQVEILSGVYEGVTTGTPIGLLIRNTDARSKDYSNIADTF
                     RPGHADYAYWRKYGVRDPRGGGRSSARLTAPTVAAGAIARKWLAHAHGITVRGYMSQL
                     GPIEIPFESWDEVARNPFFAPNASIIPQLEQFMDQLRRDGDSIGARIEVVAENPPAGW
                     GEPLYDRLDADIAHVMMGLNAVKGVSIGAGFGCIAQRGSEHGDEITPDGFLSNHAGGV
                     LGGISTGQPVTVSLAIKPTSSIRIERRSVNRAGESVSVQTLGRHDPCVGIRATPIAEA
                     MLAIVLMDHALRHRAQCSG"
     gene            complement(9089..10567)
                     /locus_tag="VFR20_11365"
     CDS             complement(9089..10567)
                     /locus_tag="VFR20_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEU0231163.1"
                     /translation="MPTDSILDLHVENSFAGLPPAFYSRVPAQPLAAPRLLHANADVA
                     ALLGLSETALDSPDFLDVCAGNRPLRGGQTLSAVYSGHQFGVWAGQLGDGRAHLLGEV
                     MAPSGALELQLKGSGHTPYSRMGDGRAVVRSSVREYLAGEAMAGLGIPTTRALALVVS
                     DDPVYRETVETAAIITRVAPSFVRFGSFEHWWNQPDNMRVLLDYVIDRFYPECRVDEA
                     GKPCSFADAALRFLETVVARTADLMAAWLTVGFCHGVMNTDNMSILGLTLDYGPYGFM
                     DGFQANHICNHTDTQGRYAWNAQPSVANWNLHRLANCLMALSLDPEALKARLAQFEPA
                     FMRAYQTRMAAKFGLSAWVEGDETLVDGWWGLLHAQRADFTLCFRRLARVEEDAEPFL
                     SLFGERQPARDWLEEYRRRLEADALPDHERAERMDRVNPIYVLRNHLAELAIRGAQKG
                     DAGEIRTLLALLRDPCTEVEGFDDYAAPAPEWACSLEVSCSS"
     gene            complement(10613..10996)
                     /locus_tag="VFR20_11370"
     CDS             complement(10613..10996)
                     /locus_tag="VFR20_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEU0231164.1"
                     /translation="MEPELNPLLRHGAVVSLLCLFVLCVLWELWLAPVRPGGSLLALK
                     AMPLLLPLRGIIRGSLYTMQWASMLILLYFMEGVVRAYSDPVPLSALLAGVEIVLSLI
                     FFLCAIFYVHPAKRAAKARARALKQ"
     gene            11080..12414
                     /locus_tag="VFR20_11375"
     CDS             11080..12414
                     /locus_tag="VFR20_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEU0231165.1"
                     /translation="MSRLHIPDPGRTAASIGKWPKNIGKVLRFSARRAAEKELPQVAA
                     SLTFTTVLAIVPLLAIVLALFTAFPLFDEFRLALENFLTANLMPPTVSLNVMGYLSQF
                     ATQASRLTVIGFLFLLVTSLMLMATVEKAFNRIWHVTRRPPLRRRMLVYWAILSLGPV
                     LAGASLWATSYVARKSFGHVTEISTSAGFALSLIPLVITGIGFAALFVGVPNRRIAWR
                     DAFVGGFCTAIALSAMKTGFAYYLIRFPSYTVIYGTFATLPIFLLWIYLSWLAILFGA
                     MITATLPLLRLRLWEESRQAGAPFIDAIKVLTLLRDARHGAPPGHGLRFLCQHLRMHQ
                     DELLSVLAALEHLGLVVVVQERNDERWVLACDPAQVDLGRVVDTLLIHRGQPGFAGNE
                     PLADALCAALKQTHTIPLENALKRGSSHPDAGVMVQNNSSGNREAIQENNHA"
     gene            12407..12862
                     /locus_tag="VFR20_11380"
     CDS             12407..12862
                     /locus_tag="VFR20_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU0231166.1"
                     /translation="MLKVSEILRVKGNTLYTATSDTSILQALETMSEQDIGSLVVMDH
                     GVLVGMLTFREIIRHLHANHGNTGANTIRSIMNDAPISVTPNTHADEVQRLMLEKHAR
                     YIPVMDGPMLLGVISFYDMAQAIVAAQQFENNMLKAYIRDWPSEEQPSV"
     gene            complement(12941..13954)
                     /gene="dusA"
                     /locus_tag="VFR20_11385"
     CDS             complement(12941..13954)
                     /gene="dusA"
                     /locus_tag="VFR20_11385"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEU0231167.1"
                     /translation="MTLHAETPADTHKSPDDWRLCVAPMIDVTDRHCRFFHRLLAPRA
                     RLYTEMITTGALMHGDVVRHLDFDPSEQPVALQLGGSEPAALAACARLGEQWGYDEIN
                     LNCGCPSERVQRGAFGACLMAEPALVADCVKAMHDAVSIPVTVKHRLGLDYQDSYDFV
                     RDFVGAQHHEGCRVFIVHARNAVLKGLSPKENRQIPPLRYDWVSRLKADFPDCRVVIN
                     GGIDQIDQALSMVGTYDGVMLGRAAWHNPGILTELSRRLDPGLALPEPGQVVHAMAAY
                     AAAEIGRGVPLRVVVKPMLGWMSGRTGARYWRRTLSDPDVLRHNDAAILERAWSDLVS
                     QAD"
     gene            complement(14025..16028)
                     /locus_tag="VFR20_11390"
     CDS             complement(14025..16028)
                     /locus_tag="VFR20_11390"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU0231168.1"
                     /translation="MRQENMAAETSWRTAEALWPQIVELLHRDADKDRFVSLLAQARN
                     LPPRAAGRGDLIRSVELAMTVKDRFELHQRRERGLLAVLETAQDLTAIVDPDRVLRAI
                     VQRARKLVGCDVGYLSIFDPERGDFYVRATDGAFSDDFKRIRVGMDVGVCGFVARNKT
                     PYSSSDYGTDNRFTHTPLIDTAMVEEHITSILGVPLLAGETVIGVLFVGDRYVRAYAP
                     WEKHILSVLAAHASVALGNARLFEQTQQALEQASGMNQKLARQTADTDTAARAHERLT
                     ALAARGGGLPEICDMVASMLSGRVAAYDDGEQQIAVADAHAMPDERDASRSLEDWGGD
                     ALHKALHESRTRGRSVAVPLAHGGFCRVAAAMGGHGMLGGLAIHTSESLRDPEVRIFE
                     RSAMVAGVVLLSREHRESVGRIEMAAMVRSLVGVPRGGLPQAMEHAARHGLDLSRPVS
                     VIVLDDLGEKAPYLSRRLANAPELAHVVMDASARSLVLLADADAVPMLEQVLPRRIRD
                     ILHREAAGVFSAPVQGPDGLRSAYGRVEQCLEVVRALKGRTRLYHEHELSLYALLFGA
                     TEGHDGVVRYLDATLGALYRQPDARKAGLARTLLAYLDSGRNVRLAAQTLDVHVNTVH
                     QRLEAVDVLVPDWRDTARALDVHVALRLWGFDDEGLVPRRPGS"
     gene            16187..17482
                     /locus_tag="VFR20_11395"
     CDS             16187..17482
                     /locus_tag="VFR20_11395"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEU0231169.1"
                     /translation="MTTDSTLPGFRTLDPAERLARLAAKVGLSDDERARVGAGLPLET
                     ADGMIENVIGKFELPYAVAGYFQVNGHDVLVPMAVEEPSVVAAASFMAKLARASGGFQ
                     TSSTGPLMRAQIQILDVTDPHGARLAILRHRQEIIDMANSRDRVLTELGGGCRDIEVH
                     VFTDTARGAMVVTHLIVDVRDAMGANTVNTMAEFVAGRLEQITAGRVRLRILSNLADL
                     RLARAHVRLPATALATREHAGGDVVNGIVDAYEFAAVDPYRAATHNKGIMNGIDPVIV
                     ATGNDWRAIEAGAHAYACRDGRYTSLSTWEIAANGDLVGTLEMPMAVGLVGGATKTHP
                     LARLSLKIMGVKTAQELAEVAVAVGLAQNLGALRALATEGIQRGHMALHARNIALGVG
                     ARGEEIDWLAREMAAARDVRADLARSLLEGHRKELGATP"
     gene            17564..18784
                     /locus_tag="VFR20_11400"
     CDS             17564..18784
                     /locus_tag="VFR20_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0231170.1"
                     /translation="MKRPLTRLAIALLAATTIAGTAAAATKDDGVIRIGFITDMSGPY
                     ADTDGPGGLEAIKMAVADAGGSVLGKKIEVLSADHQNKADIAASTARQWIDQDHIKLL
                     IGGVNSATALAMNNIVAEKKVPYINVAAATARLTNENCTPYTISYEYDTIALARGTGS
                     AVVKQGGKSWFFLVADYAFGHSLFHDTSEVVKKYGGKVMGSVKHPPNSSDMSSYILQA
                     MSSGAQVLGLANAGADTINSIKAAHSFGVTKKMKLVGLLMTINDIHGLGLETAKNLLM
                     TDSWYWNMNDASRAFAKRFYAKMHKMPSTHQAADYSAASTYLKAVAAAHTTDADKVMA
                     QLKSMKINDFYSHGYIRKDGRYIHNMYLFQVKTPAESKGPWDYLKKVATIPGDEAFNS
                     LAESTCPLIKKQSS"
     gene            18946..19911
                     /locus_tag="VFR20_11405"
     CDS             18946..19911
                     /locus_tag="VFR20_11405"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU0231171.1"
                     /translation="MAVFTPVSEHDARELLHQYSLGSLVSLRGITAGIENTNFFLTTD
                     RGEYVLTLFEVLRHDQLPFYIELMHHLAQRGIPVPEPQTCTDGTRLTSLHGKPCAIVT
                     RLTGGYEPAPGPSHCALAGETLARAHLAGRDFPIRQPNLRGLGWWLATVPSILPYLSP
                     TQADLIQHTLDEQARFAQTDIYHALPAGPSHCDLFRDNVLFGGTFDVPQIGGFIDFYF
                     AGWEKWLFDVAVSVNDWCIEQNTGVFQNDRLRAWLAAYAATRPFTPEEKEAWPVMLRG
                     AALRFWISRLYDYYLPRQAQTLKPHDPRHFERILSERHKPTAVVP"
     gene            20161..20931
                     /locus_tag="VFR20_11410"
     CDS             20161..20931
                     /locus_tag="VFR20_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HEU0231172.1"
                     /translation="MQAATLPLKSGWQWTYDGFGLFRQQPLAMFFWSLAVGLLITISY
                     LIPLLGQVALIVLTPLLTFLTLCACRNIATGKPMLPNMWLAPLKDTEASKRLLMLGLA
                     YMACCLASGFAATLPFSSSLMQAINPDGTINEILLATALRGPLIVFGLLYVLISALFW
                     HAPALIGWHRVKFAQALFFSIVACWRNKWPFLLYGVSWAVVFFALQAVGQFMVDSGMT
                     PDTAQLVLTPVNIVVAAVLYCSFYPVYESVFGANYPVR"
     gene            complement(20916..21323)
                     /locus_tag="VFR20_11415"
     CDS             complement(20916..21323)
                     /locus_tag="VFR20_11415"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEU0231173.1"
                     /translation="MRHVAKRIPGVPTAPMDELFLFPAVGFVSRLRGLHAFQGLAWGC
                     GLWIAPCGAIHTFGMGYAIDVLFLDRGHRIVRKVRHMRPNRIAWCAKARSVVELPAGY
                     CAAVPHHEKLVATALHTLTSLWGARPDINHRTG"
     gene            complement(21340..22095)
                     /locus_tag="VFR20_11420"
     CDS             complement(21340..22095)
                     /locus_tag="VFR20_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU0231174.1"
                     /translation="MAWPWILALSLVCKAFLPWRLRQTLERAVRLAGLDPVVWPPERI
                     VAAQCLAALTGSLAGWGTLFFCGPITNPMAAVYPSALLGLMCGLWPRLALRERMDRRR
                     REMLREFPFLVDMTTLCVEAGLNLHGALELAAKNIPDNPLRQELRYALADMRAGMPRL
                     EALDDMARRTDLPALRQWVTAITQADRLGMSLGPLLRAQSEQRRVERFQRAEKLALEA
                     PVKMLFPLVVCIFPCTFLVIGFPIALKLSGALS"
     gene            complement(22272..23117)
                     /locus_tag="VFR20_11425"
     CDS             complement(22272..23117)
                     /locus_tag="VFR20_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU0231175.1"
                     /translation="MSHIAVACAALVAALTFWFALRCLLRFYARYQEFFHSRAKTSLG
                     EFFLFLDPSQLWVLNLMVCGGLTLSSYVVTGSPAFAVLVGAFSLVMPWCVLKRLRRRR
                     LERLDQQLPDFLLALAGALRAGQGMQSALRQIVPQAPEPLSQEFGLMLREQRMGVALD
                     DALSGLYRRITTEGIGLVVSSLKIAMQSGGNLAETLERIAATVRARLQLLGRIRALTS
                     QGRMQAWIMALLPPGMVLILNELDPEAASALWHTTPGWLVLGAVAVLEVAGVLLIRRI
                     VDIDV"
     gene            complement(23114..24901)
                     /locus_tag="VFR20_11430"
     CDS             complement(23114..24901)
                     /locus_tag="VFR20_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEU0231176.1"
                     /translation="MLGLVLSYEDGVIETRDIPLPFDVGRATHCGVCLRAWRVARRHA
                     RFYESGPGVMVEDFGSLCGTQINGRRITQYGPLCEGDEILVGPCLIRVTRVPNSCVVS
                     PSGGGGQDTDGRSAVRMPGAAQSGRQSDQAPTSRYAGDNASAVPNVDAVTEPEVSSRP
                     VEVGLRDRLRHRRRLHGALLEALDLRRRDIAALSDEALREQAREALAAILASDTELPK
                     SMDQTELLSEVLDEAVGLGPLEPLLADPGISEIMVNRHDEIFVERAGALVRHDSGFSS
                     EQAVLGVIERIVSPLGRRIDESSPMVDARLRDGSRVNAVIPPVALRGASLTIRKFPQH
                     RPRMADLVALGSLDVSMQRFLEVCVARRKNILVSGGTGSGKTTLLNVLSNAIPVGERI
                     VTIEDAAELRLNHAHLVSMESRPANLEGSGRIEIRDLVRNALRMRPDRIVIGECRGAE
                     AFDMLAAMNTGHEGSLTTLHANSPRDALSRLETMILMAGMDLPLAAVREHVAASIDLI
                     VQQARFPDGHRAITSIVEVTGIESGRIQLQELFRLEPGEPRVFAGCGMLPDFFSGHVE
                     LPCELDLEVFRQRHPARCKDVPVVQVHTP"
     gene            complement(24905..26368)
                     /locus_tag="VFR20_11435"
     CDS             complement(24905..26368)
                     /locus_tag="VFR20_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HEU0231177.1"
                     /translation="MRVRSLAESGAWMPGVEVSRQLARPRRACLLLMCVPFLVFHGIA
                     TGQSETRLKLQVGEVRVLPVPNVARVAVGNGQIVNAVTTDEKEVILFARHDGSSSLHV
                     WSGDGRLHRYLVEVAPDGQREIQSQLRKVLERLPNVRVSLVGDKLLVEGESISDADRE
                     RISALARQYPQLLDFTSQVGWDRMVLLDVQVIEVPRNRLRELGVQWNPSSRGGFNAGL
                     VWDAGARSLAGRPADEDALMAPFPAARALGYFGVNALLSARINAMTKTGSAVVLAQPQ
                     LLARSGATAEFLAGGEVPYSTVDANGNTNTAFKPYGVSLRITPRIEHNQAVRSRIEVE
                     VSSVDSSMSAPGGPALKTRRAATEFNVRSGQTLVLAGFLSREEARNEDKIPGLGSVPI
                     IGRLFGSRRFQRNETELAIFVTPMVVPADQPDLARRIERGRAILKSAFPQPVLINVPV
                     ATSPGPSSSRRSGAGDGAWNPYGSVGSQWVEPESFKE"
     gene            complement(26365..27327)
                     /gene="cpaB"
                     /locus_tag="VFR20_11440"
     CDS             complement(26365..27327)
                     /gene="cpaB"
                     /locus_tag="VFR20_11440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEU0231178.1"
                     /translation="MRSIMSSRSLIMLVLAVCAGGVAALSMQQYLRSRVRQIEDQAKI
                     PMARRVVAAHDLPAGTRLGPDNVAVREFPASMVSSTSLAPERFTEISGSILRSALRAG
                     DSILPAHAAHAQASPFSNRLEKGRRAMTIPVDVINSVSGLLEPGDLIDLYVSFDYHRK
                     RITAPLLQGVLVLATGVNAKPVADHRPAMSGASYSSVTLDVSPEDAIKLVSARHSGTL
                     TALLRHPADGGATQKAVRGDLAGLLGVAVPAPAGRTTIPVIYGDRGSRRLPGLSPATP
                     KSPRVPPIFDLPDMPELVSAWLRSERGADGEDPVLLQSGSGARP"
     gene            complement(27352..28074)
                     /locus_tag="VFR20_11445"
     CDS             complement(27352..28074)
                     /locus_tag="VFR20_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231179.1"
                     /translation="MKACGILSGVPSARRVACLLLFLLSATAAPAADGDPVGSGFSVL
                     KTQVRVSWQDMTTMALGGVSVHYRTFFSRMSVYGTAEAFARESGMFDRVLAFKDRVVL
                     SGARNGWHWVADIGPESSGAQGLVSAMPLNPLADKGSPTPAVGAAPDWLPEGSGLYFD
                     QTLTQAGMTVRQSIHRLSQPPAAAGNAVRERLVGLGWSRMPGEPGGQSGQMEVWARER
                     SRLMFEITPDVSGSALFVQFRG"
     gene            complement(28071..28811)
                     /locus_tag="VFR20_11450"
     CDS             complement(28071..28811)
                     /locus_tag="VFR20_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231180.1"
                     /translation="MRPSGSSHACAGQALLESLVIMLALGGIFMVIPWLGRLQDIALQ
                     SAHASRFAAFSLTRDPALRPLSQIIRHFFSGPSHQWARSNGTRLISDGSQVRLSVSAL
                     SDLDVAAHPGGVDQEASALRHGLRIDEWGILMAQTGVSISNQMALPGRVRPAWLAPYP
                     SLDRGVAILVDAAHASDDVSAQRRVARSGPGWADMAQTSYSLGRRTAIVSGPVDESWG
                     RDAPLFDWLEPWAGRLPAYHLGGTGDAP"
     gene            complement(28808..30112)
                     /locus_tag="VFR20_11455"
     CDS             complement(28808..30112)
                     /locus_tag="VFR20_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231181.1"
                     /translation="MKPDKQSQRGQALVLGMLLAGVASIAFVRYFSTAQVVGARSGQV
                     HALDAAAYSGALVQARALNMLAYLNTAQIGHQIAMAHLVTLGSWSAFSTTQARQAGIG
                     NPPAPLVAMLFGAAHGAAYAASRGALGLASLADTDGELARAYAAHDDFVMRVFGHVQR
                     SVANSLPAARWQVIQAVLRQNGLRHTEPGVDGTYDVTITGDNWQGFLQTRSAGRDLRP
                     LIQQVAELYRFLGPRNHTAVNPWMVDARCPGLRHELRRRGVTELDSSGRWQSIDTQSF
                     HALRANRWIGCYRREYPMGWGWIPSAAGQTLGSPYIDTPPDDFSSQDFWRWVRQATDW
                     DLIAGDSNPLADSRAVANAHRWRGSGLPSYLDLEEDRQALRFDVTLRHPGPRGLGLVT
                     RSAAETFFARPTARADGRLEQANLFHPYWQARLVAPRSGEQP"
     gene            complement(30109..30390)
                     /locus_tag="VFR20_11460"
     CDS             complement(30109..30390)
                     /locus_tag="VFR20_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014111442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231182.1"
                     /translation="MRRVAKQNIRAREGGQGMTEYIVIVALVAVAAIAVYQSFGQVVR
                     SQTAAMARELAGEDGSEQSRAAQSAADAAATQTAARSLKSFTGNAGGGQ"
     gene            30679..31995
                     /locus_tag="VFR20_11465"
     CDS             30679..31995
                     /locus_tag="VFR20_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009911618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0231183.1"
                     /translation="MSSIPTQPGAAFDDAPSALEKAAYRKVSWRLIPFLLVCYIVAYL
                     DRVNVGFAKLQMLSDLHMSNAVYGLGAGIFFLGYFLFEVPSNVILHKVGARIWIARIM
                     ISWGIISGLTMFVTTPTMFYVMRFLLGVAEAGFFPGIILYLTYWYPAERRGTMTAWFL
                     SAVALSGFIGSPLSGWILEDLSGAHGLAGWQWMFLLEAIPSIVIGFITFFYLDDRIHH
                     AKWLSQEEKTLLERNIEADTADKHDLPLAQVFSNPRMWAMSLIYFSFVIGLYGVSFWL
                     PTIVKSTGITDSLSIGLITAIPYGFAVVAMILVGLRSDKHRERRWHIAIPAVIGAAGL
                     YLSTVWAHQTTPAIIALTFALMGIMVVLPLFWSLPTAILGGTAAAAGIAMINSLGNLA
                     GFFGPTLIGYLKTTTHSTNSGIYVLAAFMVLGALITLAMPSKLVDK"
     gene            complement(32182..32877)
                     /locus_tag="VFR20_11470"
     CDS             complement(32182..32877)
                     /locus_tag="VFR20_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU0231184.1"
                     /translation="MDTIVPVEPAATLAVVGSAARFPVRRVYCVGRNYAAHAREMGFT
                     GREDPFFFCKPADAIVNVADGQTGSIPYPPKTADLQYEIELVVVLHKGGRDLTVEQAA
                     ECVWGYAIGLDMTRRDLQGAAKKQGRPWEVGKGFDCSAPIGPVHPLEKTGTLASGAIT
                     LSVNGTRKQSGDLSDLIWTVAESIAFLSGLFELKAGDILFTGTPEGVGPVVAGDTMVG
                     AVEGLGELKVRVH"
     gene            complement(32881..33420)
                     /gene="ppa"
                     /locus_tag="VFR20_11475"
     CDS             complement(32881..33420)
                     /gene="ppa"
                     /locus_tag="VFR20_11475"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEU0231185.1"
                     /translation="MSLDRVPAGAKLPQEFNVVIEIPMNSVPVKYEVDKASGAVFVDR
                     FMLTAMHYPCNYGYIPETISDDGDPVDVLVLTPFPVNSGAVIRARAVGVLQMDDEAGG
                     DAKLLAVPVDKLYPPYSHISKPEDLPAETARIQHFFEHYKDLEKGKWVKVKAWEGVEA
                     AHKEILDGVERYKKARKEK"
     gene            complement(33450..34937)
                     /locus_tag="VFR20_11480"
     CDS             complement(33450..34937)
                     /locus_tag="VFR20_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HEU0231186.1"
                     /translation="MRTWLWIILVFVLAVALGLVLREHSGNVLIVAQPWRIELSLTLA
                     VLLLIAGFFVLYVVIRFLAWLVSGPARFRAWRGWRGEKRDHELLESGWLNVLEGRFTQ
                     GEKELAKVMGNTRSQPRKVLAGLASARASHYLGEFSRRDEALKFAREQAGTEPRLKDA
                     AATVAAEMYLDQNRPQDALVLLQPLQDASSRYFNATRLLLRAHRQLNNHDRVYDLTRL
                     LLRRGVLDKPQAMPIIETAAAARLRAGGMDAFKAIWSDLRADERTLPDVALAAACVQD
                     TQGNHDEAAKILEAAIAVNADPRLLNAYGRCPPDQVARRLGKAEVWLKSRSEDPDLLA
                     ALGNLCLIGQLWGQGEHYLLRSMKIRGDVRIHALLGNLYDRLGRQPDAMKHWRLASGV
                     AGTLPVLATNQALPSADMRSDPAPIDVARAAEPVTPHGVPMAASAVDYLVDADSAESQ
                     AVVPVSATQANPADAALDDYFDSAPIPGVDLSQTSDRPSGHSGRR"
     gene            complement(34941..36221)
                     /locus_tag="VFR20_11485"
     CDS             complement(34941..36221)
                     /locus_tag="VFR20_11485"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEU0231187.1"
                     /translation="MTESRTETPQTAQPSASAQKTSAASPSSTKSGGSAVKKKRGGWW
                     VALVIVVLIAVALAIALWYQQRQFHQTSALLSSQAQSSAQAASQASAQAQQAISMVRL
                     QASRVSALQASLQDAQEQVHALGQALQNLTDSGSDLALVNDVDHMVTIANQQLTLNGN
                     VANAIIALESAQSRLARANRPALASLQQTLNGDLDRLRAASVVDVAAVSTQLRQLDGL
                     VGHAALLIPDAAAPRLASSVSSSADAPPTPAEPGAGGTGWRHALNVVRGWWHSAWSST
                     SNELAHLVSIRRVSDPAALLMSPDQAHQLRENLHMRIMTAQLALMMRQQSVWNAETQA
                     LEQALNTRFDTKDPQTRKALELVASLARTQVAAKPPGVENSLQAIEALREAESRRGQA
                     MPSADAASTMSTHSSGNASSGSAASAASSVKPQE"
     gene            complement(36253..37062)
                     /locus_tag="VFR20_11490"
     CDS             complement(36253..37062)
                     /locus_tag="VFR20_11490"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEU0231188.1"
                     /translation="MLADISGALAILTRPQGHNDSLASQLRQAGIDVSVQPVLRLIPA
                     ATPAPVPGGFDLVMFVSAAAARFYFEQIHGGRGASWPDTTLACTVGPASARALRDAGV
                     IPAENIVHPGPESPNHDSEALWALLAPRLPAIKRALIVRGETGREWLGRQLEGAGIEV
                     RHHAAYRREPILWAAGQADELARRLSAASSSVCLVTSTESADALHANIEHHAVRLRQL
                     WERVRFVTIHPRVASRLTSRFTVASGVPPSVRLCAADDGAVFQAIQSLALS"
     gene            complement(37072..38025)
                     /gene="hemC"
                     /locus_tag="VFR20_11495"
     CDS             complement(37072..38025)
                     /gene="hemC"
                     /locus_tag="VFR20_11495"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEU0231189.1"
                     /translation="MTPSGPQKLIIATRASQLALWQAEHVRERLSALYPSCDIGLLTL
                     TTRGDQILDRTLSKVGGKGLFVKELETALLDGRADLAAHSLKDVPVDMPASFALPAIL
                     AREDFRDALVSNSVDSLAQLPTGAVVGTSSLRREAQIRARYPALRVEPLRGNVGTRLA
                     KLDRGDYAAIILAAAGLKRLGLAHRIRDYLSPEDSLPAAGQGAIAIETLATRHDVAEW
                     LAPLACADTTACAVAERVVSRVLGGSCQVPLAAFAQVQAGTLTLRALVARPDGTQVVS
                     AQMSGPVDQAQHLGELAAAELLRKGARDILASIPVSGQAQA"
     gene            complement(38128..38844)
                     /locus_tag="VFR20_11500"
     CDS             complement(38128..38844)
                     /locus_tag="VFR20_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEU0231190.1"
                     /translation="MSEFIQALHWGAIAQIVVIDILLGGDNAVVIALACRNLDARQRV
                     RGIIWGTAGAIVLRVVLIAFALFLLAVPFLKIVGAILLLWIGVKLMLPEEGHQDVAAA
                     GTIWRAVRTIIVADFVMSLDNVIAIAGAAQNTAEHHQLLYVVFGLLLSIPIIVWGSTL
                     VLKLIDRFPAVVTLGAALLGWIAGGMLMTDVFVADKIGIPSTTVKILAEIIGAVIVVA
                     VGWFLASRKNVVKENAHGSV"
     gene            complement(39015..39713)
                     /locus_tag="VFR20_11505"
     CDS             complement(39015..39713)
                     /locus_tag="VFR20_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750505.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HEU0231191.1"
                     /translation="MMTHSADFWFGLLEVIWVNVLLSGDNAVVIALAARNLPPARQRL
                     AVGGGASVAVLLRIVLTLLAARLLLLPWLKIVGAVLLVFIGVGLLAPAKPVTRPRNRR
                     SGVGVAVRAIVIADVIMSLDNVVAVAAAAQGSLTLLIIGLALSIPLVVFGSTLLLKVI
                     ERFPAIVWLGAALLGFIAGQMLVGDPAVTPLMTRIDRAASLASDVLPLFAGILGALIV
                     LGLGRLRLSRARDS"
     gene            complement(39840..40721)
                     /gene="sucD"
                     /locus_tag="VFR20_11510"
     CDS             complement(39840..40721)
                     /gene="sucD"
                     /locus_tag="VFR20_11510"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEU0231192.1"
                     /translation="MSILINKDTKVITQGITGKTGQFHTRMCRDYANGKAAFVAGVHP
                     KKAGEDFEGIPIFGSVKEAKERTGATVSVIYVPPAGAAAAIWEAVEADLDLAICITEG
                     IPVRDMLEVRNRMRDQNKKTLLLGPNCPGLITPDEIKIGIMPGHIHRKGRIGVVSRSG
                     TLTYEAVGQVTELGLGQYSAVGIGGDPINGLKHIDVLKMFNDDPDTDAVIMIGEIGGP
                     DEVNAAEWAKDNMKKPIVGFIAGVTAPPGKRMGHAGALISGGADTADAKLEIMEACGI
                     RTTRNPSEMGKLLKTVL"
     gene            complement(40744..41904)
                     /gene="sucC"
                     /locus_tag="VFR20_11515"
     CDS             complement(40744..41904)
                     /gene="sucC"
                     /locus_tag="VFR20_11515"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEU0231193.1"
                     /translation="MKIHEYQGKELLRQFGVTVPRGFPAFSVDEAMAAAEKLGGPVWV
                     VKAQIHAGGRGKGGGVKLARSLDEVRQLSSEILGMQLVTHQTGPAGQKVRRLLIEEGA
                     DIKKEYYVGIVTDRASQRVCVMASSEGGMEIEEVAAHSPEKILKEFVDPTHGLTDQQA
                     QKLARGIGVPETSVAKAAVEFQKLYKTYWETDASLAEINPLILTGSGDIIALDAKFNF
                     DSNALFRHPEIVAYRDLEEEDPAEIEASKFDLAYIQLDGNIGCLVNGAGLAMATMDTI
                     KLFGGEPANFLDVGGGATAEKVTEAFKIMLKNKGVKAILVNIFGGIMRCDVIAQGVIA
                     ACKAVNLSVPLVVRMRGTNEELGKKMLAESGLPIISADTMAEAATKAVAAAK"
     gene            complement(41987..42565)
                     /locus_tag="VFR20_11520"
     CDS             complement(41987..42565)
                     /locus_tag="VFR20_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HEU0231194.1"
                     /translation="MPLPPPDTSREPLHTRSIRVQAFARDDGQWDLEAELIDTKAYDF
                     PGADGSTHRAGDPVHHMHLRVTINDDFSITAATAAYDAAPYGPHCTAIAPDYNDLVGM
                     NLLRNFRQTVKERFGRTAGCTHLTELSYVLPTVAIQSMARQRRLAATQPAQPGAKRPF
                     QLDGCHALRVDGPVAKEFYPQWYVSPRQARRE"
     gene            complement(42769..45066)
                     /gene="metE"
                     /locus_tag="VFR20_11525"
     CDS             complement(42769..45066)
                     /gene="metE"
                     /locus_tag="VFR20_11525"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU0231195.1"
                     /translation="MTVIHNLGFPRIGAQRELKRALEAYWAGRQDVDALLDTGRDLRA
                     RHWARQADTGMAFVPAGDFAWYDQILEWTTLLGAVPARFGQPHDESVGLDTLFRMARG
                     RAPTGRPAAACEMTKWFDTNYHYIVPELVPGQAYRIARETLFEQVAQAQAQGLQVKPV
                     IPGPLTWLWLGKGDAFSDGPGDARKLALLANLLPVYGQILRRLKDMGVQWVQIDEPIL
                     VLDLPDDWKQAFARVYGKLAGSGLDILLATYFGALGDNLSVLKDLPVQGLHVDLVRAP
                     DQLDDVLAVLRSDQVLSAGIIDGRNIWRTDLDRALAVLAPAKARLGDRLWLAPSCSLQ
                     HVPVTLEGETELDAELRGWLSFAVQKLDELAWLAAALDGTASSEVQAALAAQRAALEA
                     RRVSPRTHNTVVRNRARDAAAVGRERAPFERRIESQHQALGLPTYPTTTIGSFPQTNE
                     IRALRRDWKAGSLTDAGYEKAIRAEIEHVIRFQEKIGLDVLVHGEPERNDMVEYFGEL
                     LAGFAFTRNGWVQSYGSRCVKPPIIFGDVSRPAPMTVAWTAYAQSLTDKPVKGMLTGP
                     VTILQWSFVRDDQPRQATCAQLALALRDEVVDLESAGIRVIQIDEPAFREGLPLRRGD
                     WATYLAWAVDCFRLSTSGVRDDTQIHTHMCYSEFNDIIEAIAAMDADVITIETSRSNM
                     ELLGAFEDFRYPNDIGPGVYDIHSPNVPNVDWMVDLIRKAAGRLPPERLWVNPDCGLK
                     TRAWPETEAALVAMVDAAKVLREAA"
     gene            45247..46197
                     /locus_tag="VFR20_11530"
     CDS             45247..46197
                     /locus_tag="VFR20_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004567465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0231196.1"
                     /translation="MLEIRHLETLTAIRETGSLQEAAEQLHVTQSALSHQLRDLEVRL
                     KTPLLNRRTRPARLTTAALRILTLADDVLPRLRATEHEIQRLAAGRTGRLHLAIECHS
                     CFQWLMPALDAFRQGWPDVALDLSAAFSSAPQSALLRGDLDVVITSDPQTTEAVEYSP
                     LFSYELVLAVSSTSPLARHAYIEPEQLADQVLITYPVDKQRLDVYTAFLDPAGVEPAA
                     IRKAELTPMIVQLVASHRGVAALPNWTLTEYLGQGWLRICRLGKTGVWRTLYAAVRVE
                     DADAPYLHAFLDQARTTCFATLDGIKQADGSPTPGTLRQR"
     gene            complement(46110..47162)
                     /locus_tag="VFR20_11535"
     CDS             complement(46110..47162)
                     /locus_tag="VFR20_11535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0231197.1"
                     /translation="MRLFPMFSDVQDRNVLVVGGGSVAWRKVTLLMAARACVHVGAPD
                     LCEPLRLLAERGAVRHLPGAYRDQWLEGQCLVVAATGSRPINTDVARDAGRRGLWVNV
                     VDDPALSSFHVPAIVDRSPLVVAVSSSGVAPVLARRLRERLEGLLDPALARQAELARL
                     YRTRIRARYPDVSERRRFYDWMFDGPVASHLVEHGTDGVMDGGYEDARRCLEEGLRGE
                     RQAQGLISFVDVGPGDSDLLTLRGLRELGAADVVLHEPLPDPAVLDLARRDATRHATS
                     GEVSAIDESPLLEALEQVREGARVVYLFCGRQGVPDALLGVLQERGYRCRSVPGVGEP
                     SACLMPSSVAKQVVRA"
     gene            47641..50073
                     /gene="nirB"
                     /locus_tag="VFR20_11540"
     CDS             47641..50073
                     /gene="nirB"
                     /locus_tag="VFR20_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HEU0231198.1"
                     /translation="MKKLKLVVVGNGMAGIRTLEELLKLAPDLYDVTVFGSEPHPNYN
                     RILLSPVLTGEQTLQDIILNDLNWYRDHGIDLRLGRKVVDIHRARKEVVADDGGVCAY
                     DRLLLATGSNPVILPVEGHDLDGVMTFRDIQDVHRMVEVASQRRHAVVIGGGLLGLEA
                     ASGLAARGMQACVVHLGASLLERQLDPAAAALLRANLENRGLEFLMAHQTEAILGTPD
                     NRVCGIRFSDGGERPADLVVMAVGIRPNTELAERAGIHTHRGIIVNDTLQTYDPSIYA
                     VGECVNHRGTAYGLVAPLFEQAKVAANHLALHGISRYSGSVTSTKLKVTGIDVFSAGD
                     FLGGGDTEAITLADPTGGVYKKLVIRDNKLVGACLYGDTIDGGWYFRLIKEARPIDDM
                     RDHLMLGEGSAGDTGHAGQNSVAGMSDSTEVCGCNGVCKGTIVKAIREKGLFTVDEVK
                     KHTKAASSCGSCAGLVEQILIDCVGGAANIKPRAEKPVCGCSDFTHGEVRKAIHDHHL
                     TSVRQAMDFLEWRNADGCATCRPALNYYVNCSWPGEARDDAQSRLANERMHANIQKDG
                     TYSVVPRMWGGVTSPAELRRIADVADKYQVPMVKVTGGQRIDLLGVRKQDLPAIWKDL
                     DMPSGHAYSKGLRTVKTCVGSEFCRFGTQDSTGMGIALEKNLVGMNSPHKVKLAVSGC
                     PRNCAESGIKDVGVIAVESGWEIYVGGNGGIKTEVAQFFVKVKTPEEVLEHTGAFLQL
                     YREEAFYLERTCHYLARVGLDHAKTKVIEDNANRAALFQRLKFALSFEQDPWTHRVRE
                     PAARREFETLDA"
     gene            50085..50423
                     /gene="nirD"
                     /locus_tag="VFR20_11545"
     CDS             50085..50423
                     /gene="nirD"
                     /locus_tag="VFR20_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HEU0231199.1"
                     /translation="MNHDHDTAPWTLACRIDDIPRAGSRVLKRANVDDIAIFRTWDDR
                     IFALLDRCPHKGGPLSAGLIHGHAVTCPLHGWTINLDDGQAQAPDHGCSNRIPVKVEH
                     DAVFLDLPAT"
     gene            50420..53143
                     /locus_tag="VFR20_11550"
     CDS             50420..53143
                     /locus_tag="VFR20_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0231200.1"
                     /translation="MTRGTPPTPGHALERTVSSVCCYCGTGCGVLVRSQDDQVLEVIG
                     DASHPSNQGRLCAKGLNLAATVRRDSSRVLSAELRRHRDEARRPVTLPHALSTAARKL
                     AQTLRQHGPQAIGLYLSGQLLTEDYAVFNKLARGLIGTNNIDTNSRLCMSSAVAAYTL
                     TLGADAPPACYDDLDHAGTVLIAGSNMAFTHPVLFRRLEAAKAHNPAMRIVVIDPRHT
                     DTCDIADLHLPIVAGTDVALLHAMLNVLIWEGLVDHEYIAHHTEGFETLKQRVQEFTP
                     RTAQEICGVPANDIVRCARWFGSGAPALSLYTMGLNQSSSGTAKNAALIHLHLATGQI
                     GKPGAGPFSLTGQPNAMGGREAGGMATLLPGHRKPGDPAHRADVAAVWGVDSLPEAPG
                     LPAVEMFDAVLAGTVKVLWIAGTNPAQSMPDQAKVRAALRKAEFVIVQEAYSHTETLE
                     YADLVLPAATWPEKDGTMTNSERRVSRVRAAIRPPGDARPDWQLACAVARRLAADIAP
                     DKVPLFHYRDAAEIFAEHVLLTAGRDLDHSVLSHAKLDAEGPRQWPCSPDGTSRPRLY
                     SDGRFPTVSGRAHFLDIDYTPVAEPPSAQYPFRLTTGRLRDQWHAMSRSGLVPSLTRH
                     TEEPRIHLHPDDMDRLRISDGKLVRVRSRRGEIALPALADTGLKPGCAYLPMHWGSAF
                     MAGDGINALSTDARDPVSHQPELKHSAVAIQPLAPMWEACAWVRGAVPTLRRRLARWL
                     RAFPYAVVLPSAVGGESVRLRLASLEPVPTEILAALIADLQLNEPDMAFDDPARGIAR
                     RLRRDDNGLLLAFLLAGDVRAQPPLLQWAEDGRAPGDPAGILMGRSGPVARARILCVC
                     HEVSERDILETIQAGLPLEEIQRRLKCGTACGACLPTIRRMHGEHTTRSIE"
     gene            53140..53982
                     /gene="cobA"
                     /locus_tag="VFR20_11555"
     CDS             53140..53982
                     /gene="cobA"
                     /locus_tag="VFR20_11555"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEU0231201.1"
                     /translation="MTRINDQTGSRDRPFARGLNAGKVWLVGAGPGDAELLTLKALRV
                     LQQSGIWLVDDLVGRDILAFARPGTRIIEVGKRGGCSSTPQEFILRLMGSHARQGHTV
                     ARVKGGDPFIFGRAGEEIAWLTERGIEVEAVGGLTAGLAAASALGLPLTHRTVARGVA
                     LVTASTADHTRPDWKALARSGLSIVCYMGMARPQALVAEWLAAGFAATLPAAIVQHVS
                     RPDQRQAFTTLENLAWTIEARKLASPAIIVLGEVVRYAQVNNKPESLIAGRLKQTSSM
                     GTCG"
BASE COUNT        10459 a        17793 c        16527 g         9225 t
ORIGIN      
        1 aattgcagaa ggtcggggcg cacctgatcg atgacacgat tcacctcttc gacggtcgga
       61 tccacgaaca aggcgacggc gctgacgaaa gcgggcaccg tttggcgcag ggcccgggcc
      121 tgttccacgg acaccacacg gccgctcttg ggatagaaca ctaggccaat ggcgtcagcg
      181 ccacaatcaa cagctgttcg cacgtcgttg gcgcgcgtca tgccgcaaat cttgacgcgc
      241 gtgcgtgaaa tcgtcatgga aacaccgttc agggaatcaa tgggaatgtc agtcaagtat
      301 atcgaaacag gcgacgtccc agggagggcg cccgccaggc aagcccggct cgtcacgggc
      361 cgcgtccagg gaggcaagct cgaatccaga cggatactcc acccccatga agtacaggcc
      421 cgcagccgag aacgtcggcg cggccaggcg tctgtcgcgc ccctgtagca acaaacgcac
      481 ccaatccggt gcttccctgc cctgccccac atacagcaag gccccgacca gattacgcac
      541 catgtgatga agaaacccgt tcgcggtgaa acggaaaaag aagaactcgt cgcgctgggc
      601 gatatcgagt cgccgcaggt tccggaccgg ggaatgtgcc tggcattgtg aggaacgaaa
      661 cgccgagaaa tcgtgctcgc cggccagggg gctggcggcc gcgcgcatat gcgcgagatc
      721 cagcggttgg tggacccaac ccacccgcgc atgcaacagg ggagaccgca ccctggcatt
      781 gcgcaccaca tagatgtatg tgcgcgacac cgccgagaag cgcgcatgaa aggtgtcggg
      841 caccggctgc gcccaccgca ccgcgatgct gggcggaagc agggcattgg tgccccgcac
      901 ccaggattcg agccgcctgg atgccgaggc atcgacatgc gcgacctgac ccagggcgtg
      961 cacgccagtg tcggtgcggc cggcacagac cgtcgtcacg ggttcatcga ggaagcggga
     1021 cagcgcgtca tccagtacgt cctggaccgt gcgactgtgg ggttgcgatt gccagccgtt
     1081 ccacgccgaa ccgtcgtact ccacccccag ggccatgcgt ggcatgacgt gtcaatcttt
     1141 cgctggagcg ggcccgtcgc cagacgattg gcgcagatcg aggttgatct ggtcgagctt
     1201 ctcttgaacc atggcctcgg tgatccgcgg tccgttgccg ccatcgtccc gcacggtgtt
     1261 gatgcgtcgc aatacccagg cgatgatcag tacgatcaag gccagcagcg ccgtcacgac
     1321 gcccaacatg tgttcctgaa tccagctcac gttcttgccc gatttgtttg aagccgccga
     1381 ttctcccgcg ccggcggcat gcgtgttgga agccccaccc gtactcgcct cgttgggcgc
     1441 tgacggggct gacggggctg aggaagcttc ggatgaagag gtgctgttgc ccgcagtgac
     1501 agcgccagag gcgcccgaag cagccgcttt gccggctggg tttgtcttgc ccgggacttc
     1561 gccagcctgt gaacccgtgc ccccgcccga cgcacccgat gcgttgacgt ccgccgttcc
     1621 tgccgtgccg tgggaagcgg cggaaccact cgagcccgcc atgatccctt ctgttgagcc
     1681 agacactgac ccgcccgcct cggcggcgga ggattgggcg atagtcgtgt gccccgcttg
     1741 cgtggcggat tgggcgaccg ccgcatttcc agaatgggag ggtgcgacgc caccggcgcc
     1801 ttccggatgc cctgccgccg ccatctttgt cgtcgccgtt tgtggcgtgc cctgaccgcc
     1861 tgaggcggcc gagccgccaa ccacgccggc ggaagtcacc gtccccacat ccgcgttcga
     1921 agcaccatga cccgtcgaat ccgatgatcc cgccgcaccc gatgacgatg ccgtgccacg
     1981 cgcggcattc gccagcatcg gcgccgtgac accccccgcg gacgatccgc cacccgtacc
     2041 cgcccccgac gcgttcgagg cggacgaagg cgcggaaccg tttgcggtcg cggctgtgcc
     2101 cgccccagaa gcccccgacc cgctaccttg aagcgcctga ttgagatgat gaatgttttg
     2161 ttcgagctcg gtgatgcgct gacgggaatc cgccacggcc ttgcctgtgg aggctttctg
     2221 gtcggcggcg cgatcagtgc ttgacgcgcc actggacagg accacgcggt cctgcggtgt
     2281 caccggggcg ggtttgggcg cctgggcttc ctgggagacc cgacccgccg ccgccgacgc
     2341 ccccacaacc ggaggcgtcg attcaccggc cagccgctga cgataggcat tgaactcccg
     2401 cacctgctgc atgaacaggc gccgcgcttc gcggtcggac acggctgtca gcgccgccct
     2461 gtcgggcatg gccagcgcgg cgccggcttt caccaggttg agattgtgac gaatgaacgc
     2521 ccgaggattg gcccgttgca acgcgatcat catttgataa accgtcacgc caggcacggc
     2581 gtgacggcgc gcgatagcga acatcgtgtc gccgcgccgg acccggatgg cctgggcatg
     2641 atgagtcgtg acgccatgcg tctggctgcc caggcgaggg ccagcggcca gcaccggcgc
     2701 accggcgcca cgcgcgtcgg acaagatact gatctggtac cgctgctcgc cctcggccga
     2761 gctcaccccc aacaacaaat cggcaaccga gccgtcgaat atctggttcg acgagatctc
     2821 gataagtcgg gaccttcgcc ccgagccata ggtgacgcgc acatgcatgc tgtcgaggct
     2881 gacgggcgga ttcaggcccg cctgcctcca tgcgtcgggt tcggccagcc agaccctcag
     2941 ggttgcctgc tcgacggcat ccagcccgac cagcggcaca tcgatcttca agggctgccc
     3001 cggcgccgac acaatgcgcg aatggcccag cctggcggca caggccggag cggacatcag
     3061 gagagcggcc gcaaccgccg cggccagggc ttgcgccccg gctccaattc gaaggactgg
     3121 cgtgcgacgg cgcggcggga tcatgtcaga gttcgcccag aatgatgcgc agcatacggc
     3181 gcaatggctc ggcggcgccc cacagcagct ggtcgccaac ggtgaaagca ctaaggtaat
     3241 cggaacccat ggacattttc cgcagtcgtc cgaccgggat gtccagcgtg cccgtgacag
     3301 ccacgggggt gagttcgcgc atggtgactt cgcgttcgtt cggcacgatg cgcgcccagc
     3361 gggaaccttc gcgcagcatg tcggagattt cgtcgagccc cacgtctttc ttcagcttga
     3421 tggtgagcgc ctggctgtgg cagcgcatgg cgccgatgcg cacacacagg ccatcgatgg
     3481 gcgtcggcgc cgtcccgaac gccgggccgc gcccgagaat cttgttggtc tcggcctcgg
     3541 ccttccattc ttcgcgcgaa acgccgttgc ccaggtcttt gtcgatccag ggaatcaggc
     3601 tgccggccag cgggacgccg aactggtcac aaggcaggtc ggcggcttgc tggcgtgcca
     3661 gcacaccccg gtctatggcg agaatggccg aagccggatc gtcgagcagg ccgccgactg
     3721 cattgttgag caacccgaac tggaccagca gttcgcgcat gtgctgcgcg ccgccaccag
     3781 aagccgcctg gtacgtcatg ctggtcatcc attcgaccag atcattgttg aacagcccag
     3841 ccaaccccat cagcatgcaa ctgacggtgc aattgccgcc gatgaaattc ctgacaccgc
     3901 gtccgatggc cgtatcgatg acgggccggt tcacggggtc gagcacaatg atggcatcat
     3961 cggccatgcg cagcgtgctg gccgcgtcaa tccagattcc gtcccagccc gccgcgcgca
     4021 gtttggaata cacggcggtg gtgtaatcac ccccttgggc cgtgacgatg atgggcagtt
     4081 tcttgagcgc cgcgatgtca tgcgcgtcct gcaagggcgc cgcgcccttg gcccagtcgg
     4141 gcgccgcccc acccgcattg ctggtggaga agaaaacagg ttcgaatagg gaaaaatcat
     4201 tttcgtcgcg catgcgctgc atgaggaccg aacccaccat gccacgccag ccgaccaaac
     4261 ccactgcttg agtcatgtcg aatcgcttga agtcagaact acgaatattg tcggtcaaaa
     4321 ccgccttgaa ccgcccgccg gacaacgtcc ggccagcccg agacccctat tttaatgcgt
     4381 ggagaaccgc atcccccatt tgcgaggtgg acacccgggt cgtgccctct tcgaaaatgt
     4441 cggcggtgcg caacccttgc cgcagaaccg cccgcaccgc ctgttcgata cgctcggcca
     4501 cggcccccgc attcagggaa taacgcagca acatggcggc cgacagaatc gtggccaagg
     4561 ggttggcgac gttcttgccc gcgatgtcag gtgccgaccc gtggctgggt tcatacaacc
     4621 cttggttcga cgcgttgagc gaggccgagg gcagcatgcc gatggaaccg gtgagcatgg
     4681 cggcctcgtc agacagaatg tcgccaaaca ggttacccgt cacgatgacg tcgaattcct
     4741 tgggcgcccg caccagttgc atggcggcgt tgtcgacata catgtgcgag agctcgacat
     4801 ccggatattc ctggctgacg tcgatgacga tgtcgcgcca gaactgcgag gtctccagca
     4861 cattggattt gtccacgctg caaagcttgc cgcgacgctt gctggcggcc tggaaaccca
     4921 cgtgggcgat gcgccgcact tccgattcgg cgtagtgcat ggtgtcgaaa ccctgcagtt
     4981 caccggcgaa cggcccttcc ggtgccttgc gcacaccgcg cggctggccg aaataaatgt
     5041 cgccggtgag ctcgcgcacg atgagaatat cgagccccga caccacttcc gggcgcagcg
     5101 aagacgcgtt ggcgagctcc ggatacagaa tggccggccg caggttggcg aacaggccca
     5161 gcgccttgcg caggccgaga atggcttgct cgggccggag ttcgcgggcc aggtgatcgt
     5221 acttccagtc ccccaccgcg ccgaacagca cggcgtcgga acgtttcgcc aattcgaggg
     5281 tcgcgggcgg caacggatgc cccgcggcat cgtaggccgc gccaccgacc ggcgcgcttt
     5341 ccagctcgag atccaggccc agcgcggtca atacgcggac agcctgctcg gtgatttcag
     5401 tgccaatgcc gtcgcccggc aaaacagcga tcttgtgcgt catgtacttt tccagaacgt
     5461 tgcattcaag caacaacaca ttcactaaaa ggccaaggcc gcattcgcgg ccccggcgca
     5521 tccttcacga tacgcggcac ctctcggtgc cgcgcggcat tgccaggaga gccctccgtt
     5581 gcgcgtcacg aacgcacacg gcccctggcc atacaggcaa ctacgccacc gggcggccct
     5641 ccagccaggg gtggcgcgcc aaatgctcgg actcaaaagc ccggatcaga tcggccttcc
     5701 gcagtgtcag cgcgatgtcg tcgagtccgt taactaaaca atgcttgcga aacggctcga
     5761 tgtcaaagcc cagtttccgc ccttcgggcg tgatgatggc ctgctcgggc agatcgatgc
     5821 gcaaggtata cccggaaaac gcgcggactt catcgaacag gcgggaaacc tcgagttcgg
     5881 acaacacgat gggcagcagg ccgttcttga aactgttgtt gaagaaaata tcggcatagg
     5941 acggtgcgat gatcgcccgg aacccatact gggccaacgc ccacggcgcg tgctcgcgac
     6001 tggatccaca gccgaaattc ttgcgcgcaa gcagcacaga cgccccctgg tagcgtggct
     6061 ggttcaacac gaagtccgga ttcagtggac gccgggaatt gtccatgccc ggttcgccat
     6121 ggtccagata acgcaattca tcgaacagat tggggccgaa ccccgtgcgc ttgatggatt
     6181 tcaggaactg cttgggaatg atgagatcgg tatcgacatt ggcgcgatcg agaggcgcca
     6241 cgatgccttc gtgagtggtg aatgcctgca tggcggtctc ctagcgaaaa ttcctgacat
     6301 cgacgaaatg gccggcgatg gccgccgcgg ccgccatggc cgggctcacc agatgggtac
     6361 gcccaccctg gccctgccgc ccttcgaaat tgcggttcga ggttgaggcg caacgctcgc
     6421 ccggttcgag acggtcggca ttcatggcca ggcacatgga acagcccggc tcacgccatt
     6481 cgaaacccgc ccccaggaat atcttgtcca gcccttcttt ttcggcctgc gccttgacca
     6541 ggcccgagcc gggaacgacc atggcctggc gcacattggc ggccacccgc ttgccgcgcg
     6601 ccacttgcgc cgcggcgcgc aggtcttcga tgcgcgcatt ggtgcatgaa ccgatgaaga
     6661 cgcggtctat cttgatgtcg gtgagcgggg tgttcggttt gagccccatg tattccagcg
     6721 cgcgctccat gccgccgcgc ttgacgtcgt ccttttcctt gtctggatcg ggcacgcgcg
     6781 catcgacggc gagcaccatt tcaggcgacg tgccccaggt gacctgcggc gcgaccttgc
     6841 gtgcgtcgat ctgcacgacc cggtcgaacg tcgcgccggg atcggaatgc agggtgcgcc
     6901 agtatttgac ggcctggtcc cacagaacgc ctgtgggggc gtacggccgc ccgcggaagt
     6961 attcgatggt cttgtcgtcg acggccacca tccccgagcg cgcgccagcc tcgatggcca
     7021 tgttgcagac tgtcatgcgg ccttcgaccg acaaggcccg gatcgtggcg cccgaaaatt
     7081 cgatggcgca cccggtgccg cccgccgtgc caatctggcc aatgatgtag agcaccaggt
     7141 ctttggcggt gcagccaaat ggcaattcgc cctcgacatt gatctgcatg cttcggcttt
     7201 tcttcatgag cagcgtctgg gtcgccagga cgtgttcgac ctcggaggtg ccgatgccga
     7261 aggccagcgt gcccagcgcg ccatgcgtgc tggtgtgcga atcgccgcac acgaccgtca
     7321 tgcccggcag cgtcgcgccc tgctccgggc cgatgatgtg gacaatgccc tggcgccggt
     7381 cattcatgcg gaactgcgtg acaccgtatt tttcgcaatt gctgtccagc gtgtcgacct
     7441 gcaggcgcga gacgggatcc tggatgccct ggtcacgcgc cgtggtgggt acgttatggt
     7501 cggaaaccgc cagattcgcg ctcacgcgcc atggcttgcg gccggccagc gccaggcctt
     7561 cgaacgcctg tggactggtg acttcatgca ccaggtgacg atcgatgtac aggagacagg
     7621 ttccatcgga ctcttgatgc acgatgtgtg cattccagag tttgtcgtac aaggtttggg
     7681 ccatggcaat gctcgtggga aaggaagtgg agaatatagg ttccagggat cctcggacgc
     7741 atgccgcgcg gtagcggtgt tcagaggatt cctgaacatt atgccacccc tgtaacctag
     7801 tacaagacca gttcttatac tggtactgag actgctatcc tgagcattgc gcgcgatgcc
     7861 gcagcgcatg atccatcagc acgatggcca gcatcgcctc cgcgatgggc gtggcgcgaa
     7921 tccccacaca ggggtcatgc cgacccagcg tttgcacgga gacagactcc ccggcgcggt
     7981 tgacggatcg gcgttcgatg cgaatgctgg acgtgggctt gatggccagt gacacggtga
     8041 cgggttgccc ggtggaaatt ccgcccagga caccgcccgc gtgattgctc aggaagccat
     8101 cgggggtgat ttcatcaccg tgttcggacc cacgctgagc aatgcagcca aaacccgcgc
     8161 cgatggacac gcccttgacg gcattcagac ccatcatcac atgcgcgatg tcggcatcga
     8221 ggcggtcgta aagaggttcg ccccagccag cgggcggatt ttcggccacc acttcgatac
     8281 gggcaccgat ggagtcgcca tcgcggcgca gctgatccat gaactgttcg agttgcggaa
     8341 tgatcgaggc attgggtgcg aagaacgggt tgcgggcgac ttcgtcccac gattcgaacg
     8401 gaatttcaat gggaccgagc tggctcatgt agccgcgtac cgtgatcccg tgggcatgcg
     8461 ccagccattt gcgcgcgatg gcgcccgccg cgaccgtcgg cgccgtgaga cgcgccgacg
     8521 agcggccgcc cccgcgcggg tcgcgcaccc cgtatttgcg ccagtaggca tagtcggcgt
     8581 gaccagggcg aaaggtgtcg gcgatgttgc tgtaatcctt gctgcgcgcg tcggtgttgc
     8641 gaatgagcag gccaatgggt gtgcccgtgg tcaccccttc gtacaccccc gacaggattt
     8701 caacttgatc cgcttcctgg cgctgcgtga catgacgcga cgtgcccggg cggcgccgat
     8761 cgagttcctt ctgtatgtcg gtctcgctca acggcaatcc gggcgggcag ccatcgacca
     8821 cggcgccaat ggccgggccg tgggattcgc cgaagttggt gacacagaag agcttgccta
     8881 gagtattgcc ggacatgggg aaggacgctg tgaagagaac acaaatggaa gaatgcggac
     8941 aatgatgcgg aagcgccatt gacgccaaac gctgacgcct tgaaaccaca aatcccggac
     9001 acccgcattg agaacaacca acatcattgt atcggttgtg ccagcgttca ggcgacgttg
     9061 agcgacatcc gggcttgagc tgcgggggtc aggaagaaca actgacctcc agcgagcagg
     9121 cccattcggg cgcgggtgcc gcgtaatcgt cgaagccctc cacttccgtg catgggtcgc
     9181 gcaacagcgc cagcaacgtg cggatctcgc ccgcatcgcc cttctgcgcg ccacgaatgg
     9241 cgagttcggc cagatgattg cgcaacacat aaatggggtt gacacgatcc atgcgctcgg
     9301 cgcgttcgtg gtccggcaag gcatccgcct cgaggcggcg gcgatattcc tcgagccaat
     9361 cccgggcggg ttggcgctcg ccaaacaggg aaaggaacgg ttccgcgtct tcttccaccc
     9421 gcgccaggcg acggaaacac agagtgaaat cggcgcgttg ggcatgcagg agcccccacc
     9481 agccgtccac cagggtttcg tcaccttcca cccaggcgct caagccgaat ttggcggcca
     9541 tgcgcgtctg gtaggctcgc atgaacgcgg gttcgaattg cgccagccgg gctttgagcg
     9601 cctcgggatc caggctcagt gccatcaggc agttggccaa ccggtgcagg ttccagttgg
     9661 cgacggacgg ctgggcattc caggcgtagc gcccctgcgt gtcggtgtga ttgcaaatgt
     9721 ggttggcctg gaacccgtcc atgaatccat acggcccgta atccagcgtc aggcccagta
     9781 tggacatgtt gtcggtgttc atgacgccat ggcagaaccc caccgtcagc caggctgcca
     9841 tcagatccgc ggtccgggcc accaccgtct ccagaaaccg cagcgccgca tctgcgaatg
     9901 agcaaggctt gccggcttcg tcgacacggc attcgggata gaagcggtca atgacgtaat
     9961 cgagcagcac ccgcatattg tcgggttggt tccaccaatg ctcgaacgaa ccgaaacgca
    10021 cgaagctggg cgcgacccgc gtgatgatgg ccgccgtttc cacggtctcg cggtaaacgg
    10081 gatcatcgga caccaccaac gccagcgccc gggtggtggg aatacccagg ccagccatgg
    10141 cctcccccgc cagatattcg cgcacggacg aacgcaccac cgcacggccg tcacccatgc
    10201 gcgagtacgg cgtgtggccg gagcccttga gctggagctc cagggctccg gagggtgcca
    10261 tgacctcgcc aagcaagtgg gcgcgaccat cgcccaactg accggcccac accccgaact
    10321 ggtgaccgct gtagaccgcc gacagcgtct gcccgccccg caatggacga ttcccggcgc
    10381 agacatccag gaagtcgggg gaatccagcg cggtttcgga caagcccaac agcgccgcca
    10441 cgtcggcatt ggcatgcaga agacgcggcg ccgccaaggg ttgcgcaggc acgcgcgaat
    10501 agaacgcggg cggcaagccg gcgaatgagt tttcgacatg cagatcgaga attgaatcag
    10561 tgggcatcac aaacctttca ggactgggcc ggcactccgg tatccggagt cactattgct
    10621 tgagcgcgcg cgcccgggcc ttggccgcgc gcttggccgg atgaacgtag aagatcgcgc
    10681 acagaaagaa gatcagtgac aagacgatct ccacacccgc cagcagcgcg gacaggggca
    10741 cagggtcgga gtaggcgcgc accacacctt ccatgaaata cagcaggatc agcatcgatg
    10801 cccattgcat ggtgtagaga ctgccgcgga tgatgccgcg caagggcagc aacaacggca
    10861 tggccttgag cgcaagcagc gatccgcccg gccgcaccgg cgccagccag agctcccaga
    10921 gcacacatag gacgaacagg cacagcaacg acacgaccgc gccatgacgc agaagaggat
    10981 tcagttcagg ttccatactg ctattatcac tcgaacagac actgccaact ccggccgggg
    11041 ccggcccgcc acgccccata cccgggacac cgccccagca tgagccgatt gcacattccc
    11101 gacccgggcc gtacggccgc cagcatcggc aaatggccga aaaacatagg gaaagtcctg
    11161 cgcttttcgg cgcggcgcgc agccgaaaaa gaactgcccc aggtggccgc cagcctgaca
    11221 ttcaccaccg tcctggccat cgttcccctg ttggccatcg tgctggcact gttcaccgcg
    11281 tttcccctgt tcgatgaatt ccgcctggcc ctagagaact tcctgaccgc gaacctgatg
    11341 ccccccacgg tatcgctcaa cgtcatgggc tatctgagtc agttcgccac gcaggcatcc
    11401 cggctcaccg tgatcggctt tctgtttcta ctggtcacgt ccctgatgct catggccacg
    11461 gtggaaaaag cattcaaccg catctggcac gtgacgcgac gaccgccgct gcgccgccgc
    11521 atgctcgtct actgggccat cctgtcgctc ggccccgtcc tggcgggcgc cagcctctgg
    11581 gccacgtcgt acgtcgcacg caaatcgttc gggcacgtca cggaaatctc caccagcgcg
    11641 ggtttcgcgc tgtcgctcat cccgcttgtc atcaccggca taggtttcgc cgccctgttc
    11701 gtgggcgtac ccaaccgccg catagcctgg cgggacgctt tcgtgggtgg cttctgcacc
    11761 gccatcgcgc tgagcgcaat gaaaaccgga ttcgcctatt acctgatccg gttcccctcc
    11821 tacaccgtga tctacggcac cttcgccacc ctgcccatct ttctgctctg gatctacctg
    11881 tcctggctgg cgattctgtt tggtgccatg atcactgcga cgttgccatt gctgcgtctg
    11941 cgcctttggg aggaaagcag gcaggcgggc gccccgttca tcgacgccat caaggttctg
    12001 accctgctgc gcgatgcccg acacggcgcg ccgcccggac acggcctgcg cttcctctgc
    12061 cagcacctgc gcatgcatca ggatgaactc ctgtccgtgc tggcagccct ggagcacctg
    12121 ggactggtgg tggtcgtgca ggagcgcaac gatgaacgct gggtgctggc ctgcgacccg
    12181 gcacaggtcg acctgggtcg ggtcgtcgat acgctgctga tccaccgcgg ccagcctgga
    12241 ttcgccggca acgagcctct ggcggacgcc ctgtgcgccg cgctgaagca aacacacaca
    12301 attccgttgg aaaacgccct gaaacgaggg tcgtcgcacc cggacgccgg cgttatggtg
    12361 caaaataaca gttccggaaa ccgggaagcc atccaggaga acaaccatgc ttaaggtcag
    12421 cgaaattctg cgcgtcaaag ggaacaccct gtacacggca acctcggaca cctccattct
    12481 gcaggcgctc gaaaccatga gcgaacaaga tatcggctcg ctggtcgtca tggatcacgg
    12541 cgtgctcgtg ggcatgctca cgttccgcga aatcatccgc cacctgcatg ccaaccatgg
    12601 caacaccggc gccaacacga ttcgcagcat catgaacgac gcgcccatca gcgtcacccc
    12661 caacacccac gccgacgaag tccagcgcct gatgctcgaa aaacacgcgc gctacatacc
    12721 ggtcatggac gggcccatgc tgctgggcgt gatctcgttc tacgacatgg cccaggccat
    12781 cgttgccgca cagcaattcg agaacaacat gctcaaggct tatatccggg actggcccag
    12841 cgaggaacag cccagtgtct gatgctggcc atggccactg ggctcgtcca cattgaaaat
    12901 cagtgcgtgc aacgcgtccg ccgcgtgtgg tgggtttcgc ttaatccgcc tggctgacca
    12961 gatccgacca cgcccgttcc agaatggccg cgtcgttgtg ccgcaacaca tcggggtcgg
    13021 ataacgtccg gcgccagtaa cgggcgcccg tgcgcccgct catccagccc agcatgggtt
    13081 tgaccaccac ccgcagcggc acgcccctgc cgatttccgc ggcggcgtag gccgccatcg
    13141 catgcacgac ctgcccgggc tccggaagcg ccagccctgg gtccaggcgc cgagacagtt
    13201 ccgtcaggat gccgggattg tgccaggccg cccgccccag catcacgcca tcgtatgtgc
    13261 cgaccataga caacgcctga tcgatctggt cgataccgcc attgatgacg acgcggcaat
    13321 cggggaaatc ggccttgagc cgggacaccc agtcgtaacg cagcggaggg atctgccggt
    13381 tctctttcgg agacaagccc ttgagcacgg cgttgcgcgc gtgcacgatg aatacgcgac
    13441 acccctcgtg gtgctgggcg cctacgaaat cgcgcacgaa atcgtacgaa tcctgataat
    13501 ccagccccag acggtgcttg accgtgacgg gaatcgacac ggcgtcatgc atggccttga
    13561 cgcaatcggc gacgagggcc ggctcggcca tcaggcatgc gccgaacgcc ccgcgctgga
    13621 cgcgctcgga cgggcagccg caattcaggt tgatctcgtc gtaaccccat tgctcgccca
    13681 ggcgggcgca tgccgccagc gcggcgggct cgctcccccc cagttgcagc gccacaggct
    13741 gctcggaggg gtcgaagtcc agatggcgca ccacgtcacc gtgcatcagg gcaccggtgg
    13801 tgatcatctc ggtgtacagg cgggcccgcg gcgccagcag ccgatgaaaa aaccggcaat
    13861 gccggtcagt gacatcgatc atgggggcga cgcacagtcg ccagtcatcg ggcgatttgt
    13921 gcgtatccgc cggagtttcg gcgtgaagcg tcatggtttg aaaatctgtt gcgaaatcgg
    13981 cccccgctgg agccagggaa tgccgatatt gtaccggcgg ccattcagga acccggacgc
    14041 cgcggcacca acccctcgtc atcgaagccc cacaagcgta acgcgacatg gacgtccagt
    14101 gcgcgcgcgg tatcacgcca gtccgggacc agaacatcaa ccgcttccag cctctgatgc
    14161 accgtgttga cgtgcacatc cagggtttgc gccgcgagac gcacattgcg gccgctatcg
    14221 agatacgcaa gcagggtacg ggccagaccc gccttgcggg catcaggctg tcggtacagc
    14281 gccccgagcg tggcgtccag gtagcggacg acgccatcgt ggccttcggt ggccccgaac
    14341 aacaaggcgt agagcgacaa ttcatgctcg tgatagagcc tcgttcgacc tttcagcgcg
    14401 cgcacgacct ccaggcattg ttccactcga ccatacgcgg accgcaaccc atcgggccct
    14461 tgaacgggtg ccgagaaaac gccagcggcc tcccgatgca gtatgtcacg tattcgacgc
    14521 ggcaagacct gttccagcat ggggaccgcg tcggcgtcgg cgagcagcac caacgacctt
    14581 gccgaggcat ccatgacgac gtgcgcgagt tctggcgcgt tggcaagccg gcggctcagg
    14641 tatggggcct tttctcccag atcgtccagg acgatcacac tgaccggccg cgacaagtcc
    14701 aggccatgcc gcgcggcatg ctccatcgcc tgtggcaggc caccgcgcgg caccccgacc
    14761 agactacgca ccatggccgc catctcgatg cgcccgaccg actccctgtg ctcacgcgac
    14821 agaagaacca ccccggccac catggcgctg cgttcgaaga tccgcacctc cgggtcgcgc
    14881 aatgattcac tggtgtgaat ggccaaccca cccagcatgc catgcccgcc catcgccgcc
    14941 gcgacacggc agaatccgcc atgagcgaga ggcaccgcca cggatcggcc gcgcgttcgg
    15001 ctttcatgaa gcgccttgtg caaagcatcc ccgccccagt cttcgaggga tcgcgatgca
    15061 tcccgttcgt ccggcatggc atgcgcatcg gcgaccgcga tttgctgttc gccgtcgtca
    15121 taggccgcca cgcgcccact cagcatgctg gcgaccatgt cgcagatctc gggcagaccg
    15181 cccccgcgcg ccgcgagagc cgtcaggcgt tcgtgagccc gcgccgccgt atcggtgtcg
    15241 gcggtctgac gcgccagctt ctgattcatg ccactggcct gctcaagcgc ctgctgggtc
    15301 tgctcgaaca gcctggcatt gcccagcgcc acggaagcat gtgccgccag tacggacaag
    15361 atgtgttttt cccagggcgc gtaggcgcgc acataacggt ccccgacaaa cagcacgcca
    15421 atgaccgtct cacccgccaa cagcggcaca cccagtatcg aggtaatgtg ttcctcaacc
    15481 atggccgtgt cgatgagcgg cgtatgggtg aaacgattgt cggtgccata gtcggatgag
    15541 gaatacggtg tcttgttgcg tgcgacaaag ccgcataccc ccacatccat gccgacccgg
    15601 atccgcttga aatcatccga aaacgcgcca tccgtggcac gaacgtaaaa atcgccccgc
    15661 tccggatcaa aaatggacaa atagcccacg tcgcagccca ccagcttgcg ggctcgctgg
    15721 acaatggccc gcaggacacg atccggatcc acgatggcgg tgaggtcctg cgcggtttcc
    15781 agcaccgcca gcaagccgcg ctcgcgccgc tggtgcaatt cgaaacggtc cttgaccgtc
    15841 atggccaatt ccacgctccg aatcagatcg cctctgccgg ccgcccgagg gggcaggttc
    15901 cgagcctgcg cgagcaacga gacgaaacga tccttgtcgg cgtccctatg cagcaattcc
    15961 acgatctggg gccaaagggc ttcagcggta cgccagctcg tttcggccgc catgttttct
    16021 tgtctcatct gaatttccag tgcgctatgt atgaattcac ataaatttta ttttgaaatg
    16081 gtatttcaca acatatattt cgcccacaca tcctttcata ctggcctctt ccggcaaccc
    16141 ctgggccatt gcgtcccccc tttcgccaac cccctgacac caaaccatga cgacagactc
    16201 cacccttccc ggttttcgca cccttgatcc cgccgaacgc ctggcacgcc tggccgccaa
    16261 ggtcggcctg agcgacgacg agcgcgccag agtcggcgcc ggcctgcccc tggaaaccgc
    16321 cgacgggatg atcgagaacg tcatcggcaa atttgaattg ccttatgccg tggccggcta
    16381 tttccaggtc aatggccacg atgtgctggt tcccatggcg gtcgaagagc cgtcggtggt
    16441 ggctgcggcc tcattcatgg ccaagctggc ccgggcatca ggggggttcc agacgtccag
    16501 caccggcccg ctcatgcggg cccagatcca gattctggat gtgacggacc cacatggcgc
    16561 acgcctggcg atcctgcggc atcgccagga aatcatcgac atggccaata gccgggaccg
    16621 cgtgctgacg gaactgggcg gtggttgccg cgacatcgaa gtccacgtct tcaccgatac
    16681 ggcgagaggc gcgatggttg tcacgcatct gatcgtggac gtgcgcgacg ccatgggtgc
    16741 gaacacggtc aacaccatgg ccgaattcgt cgccgggcgg ctcgaacaga tcaccgccgg
    16801 acgcgtccgc ctgagaatcc tgtcgaacct cgccgatctt cgactggccc gcgcccatgt
    16861 tcggctcccg gccacggcgc tggccacccg tgagcacgcc gggggcgacg tcgtcaacgg
    16921 gatcgtcgac gcctacgaat tcgcggcggt ggatccatac cgggcggcca cccacaacaa
    16981 gggcatcatg aacggcatcg accccgtcat cgtggccacc ggcaatgact ggcgcgcgat
    17041 cgaagccggg gcgcacgcct acgcctgccg cgacgggcgc tatacatcgc tcagcacctg
    17101 ggagatcgcg gcgaacggcg acttggtcgg caccctggaa atgccaatgg ccgtcggcct
    17161 ggtcggcgga gccacgaaaa cacaccctct ggcaaggctt tcgctgaaga tcatgggggt
    17221 gaagaccgcc caggaactgg ccgaggtggc cgtggccgtc ggcctggcac agaacctggg
    17281 cgcgttgcgg gcgctggcca ccgagggcat acagcgcgga cacatggccc tgcacgcccg
    17341 caacatcgcg ctcggcgtgg gagcccgggg cgaggaaatc gactggctgg cccgcgagat
    17401 ggcggccgcc cgggacgtgc gggcggatct ggcccgctcg cttctcgagg gtcatcgcaa
    17461 agagctgggc gcgacccctt gagccgccgc gtgacggcac acccaatcaa cgttgcacaa
    17521 tgacggccga cgaaggcccg cacacactaa ccggagacac cacatgaaac gaccactcac
    17581 ccgactcgcc atcgccctgc ttgccgcgac aacaatcgcc ggcaccgcgg ccgccgccac
    17641 caaggacgac ggcgtgatcc gcatcggctt catcaccgac atgtcgggtc cctatgccga
    17701 cacggatggc ccgggcggcc tggaggccat caagatggcc gtggccgatg ccggtggcag
    17761 cgtgctgggc aagaagatcg aggtattgtc ggcggatcac cagaacaagg ccgacatcgc
    17821 cgcctcgacc gcgcgccagt ggatagacca ggatcacatc aagctgctga tcggcggcgt
    17881 gaattcagcc accgccctgg cgatgaacaa catcgtggcc gagaagaagg tgccatacat
    17941 caacgtcgcc gccgccacgg cccgactcac caacgagaac tgcaccccct acacgatcag
    18001 ctatgaatac gacaccatcg cgctggcccg gggcaccggc tcggcggtgg tcaagcaagg
    18061 tggaaaatcc tggtttttcc tggtcgcgga ctatgccttc ggccattcgc tgttccacga
    18121 cacctcggaa gtggtcaaga agtacggcgg caaggtaatg gggtcggtca agcatccgcc
    18181 caactcctcc gacatgtcgt cgtacattct tcaggccatg tcctccggcg cgcaggtgct
    18241 gggcctggcg aacgcggggg ccgacaccat caattcgatc aaagcggcgc acagcttcgg
    18301 agtcacgaag aagatgaagc tcgtggggct gttgatgacc atcaacgaca ttcatggcct
    18361 cggcctggaa acggccaaga acctgctcat gacggatagc tggtactgga acatgaacga
    18421 cgcctcgcgc gccttcgcca agcgcttcta cgcgaagatg cacaagatgc ccagcaccca
    18481 ccaggcggct gactattcgg cggcgtccac ctacctgaaa gctgtcgccg ccgcgcacac
    18541 cacggatgcc gacaaggtca tggcccagct caagagcatg aagatcaacg atttctacag
    18601 tcatggatac atccgcaagg acggtcgata catccacaac atgtacctgt tccaggtcaa
    18661 gacgccggcc gaatcgaaag gcccgtggga ttacctgaag aaggtggcca ccattcccgg
    18721 agacgaggcc ttcaacagcc tggccgagtc cacctgccca ttgatcaaga agcaatcgag
    18781 ctgatcaaat tgcacgatgg gaacccgcgg tcgacgatgc gcccgcacag gctcctggac
    18841 gaaaagcaca agccgtcggg cggaaaacgc cgaacgcgaa cctagtctaa aatcgacaag
    18901 cccacatacc gtgggctttt tccatctctc tcccgcgtag cgcccatggc tgtattcacc
    18961 cccgtcagcg aacacgatgc ccgcgaactc ctgcaccagt actcgctggg cagtctcgtt
    19021 tcgctgcgtg gcatcaccgc cggcatcgag aacacgaact tcttcctgac gacggatcgc
    19081 ggcgaatacg tgctgacgct cttcgaagtg ctgcggcacg accagctgcc gttctacatc
    19141 gaactaatgc atcacctggc ccagcgcggc attcccgtgc cggaacccca gacttgcacc
    19201 gacggcacgc gcctcaccag cctgcacggc aaaccctgcg ccatcgtcac ccgtctcacg
    19261 ggtggctacg aacccgctcc cggcccatcg cattgtgcgc tggccggtga aacactggcg
    19321 cgcgcgcatc tcgccggccg cgacttcccc attcggcagc cgaatctgcg cggattgggt
    19381 tggtggctgg cgacggttcc ctcgattttg ccgtacctga gcccgaccca ggccgacctc
    19441 attcagcaca cactggacga acaggcgcga ttcgcccaaa ccgacattta tcacgcgcta
    19501 ccggccggcc catcacactg tgatctgttc cgcgacaacg tcctgttcgg cggaacgttc
    19561 gacgtgcctc agattggcgg cttcatcgac ttttatttcg cgggatggga gaagtggctg
    19621 ttcgacgtcg cggtcagtgt caacgactgg tgcatcgaac agaacaccgg cgtgttccag
    19681 aacgatcggc tgcgcgcgtg gcttgccgcc tatgcggcca cgcgcccgtt cacgcccgag
    19741 gaaaaagaag cctggccagt gatgctgcgg ggggcggcgc tgcgtttctg gatctcgcgc
    19801 ctgtacgact attatctgcc gcgccaggcg cagacgctca aaccccacga ccctcgtcat
    19861 tttgaacgca ttctgtcaga gcggcacaaa ccgacagcgg tggttccctg agacagcgcc
    19921 gtatctgttt ccctgattca tgagtatcct tgatccatcg cgggcctggc caccccatga
    19981 aaccaaccat tattccgcac gacttcacca ccggccacct gttgcgtaaa atcacgacag
    20041 gacacggaac cagtatggtg attgccgcta tactcggttg ccaccagaca aacaccgcgg
    20101 ctcgctcacc attcagtcgg gccacggacg catcatccag attggggaat ctccacaacc
    20161 atgcaagcag ctaccctgcc cctgaaatcc ggctggcaat ggacctacga tgggtttggg
    20221 ctatttcgcc agcaaccgct ggccatgttt ttctggagcc tggcggtggg gctgctgatc
    20281 accatcagct acctcatccc gctgctcggg caagtggcgc tgatcgtcct taccccgctg
    20341 ctcacgtttc tcactctctg cgcgtgccgc aacatcgcca ccggcaaacc catgctgccc
    20401 aacatgtggc tggcaccgct caaagacacc gaagccagca agcgcctgtt gatgctcggg
    20461 ttggcctaca tggcctgctg cctggccagc gggttcgccg ccacgctgcc gttctcgagc
    20521 agcctgatgc aggccatcaa tcccgacggc accatcaacg aaatactgct ggccaccgcg
    20581 ctgcgcggcc cgctgatcgt gttcggcctg ctgtacgtgt tgatctcggc cctgttctgg
    20641 cacgcgcccg cgcttatagg ctggcaccgc gtcaagttcg cccaggccct gttcttttcc
    20701 atcgtggcct gctggcgcaa caaatggcca ttcctgctgt atggcgttag ttgggcggtc
    20761 gtgttcttcg cgctgcaggc tgtgggccag ttcatggtgg attcgggcat gacgccagac
    20821 acggcgcaac tggtgctgac cccggtcaac atcgtggtcg ccgccgtgct gtattgcagc
    20881 ttctacccgg tgtatgagag cgtgttcggg gcgaattatc ccgtgcggtg attgatgtcg
    20941 ggtcgggcgc cccacaagga agtcaatgtg tgcaacgccg tggcaaccag cttctcgtgg
    21001 tgtggaaccg ccgcacaata gcccgccggc agctcgacca ccgatcgcgc cttggcacac
    21061 caggcaattc tattcggccg catgtgtcga accttccgca cgatgcggtg cccacggtcc
    21121 agaaacagca catcgatggc ataacccatg ccgaacgtat ggattgcgcc acaaggcgcg
    21181 atccaaaggc cgcatcccca cgccagtcct tgaaaagcgt gcaggccgcg caatctggat
    21241 acgaacccga cggccgggaa gaggaacagt tcatccatgg gagcggtcgg cactcccgga
    21301 atgcgttttg ccacatggcg catgccggta tccatcacgt caagacagcg cgcccgacaa
    21361 tttgagcgcg atgggaaagc cgatgaccag gaacgtgcag gggaagatgc acaccaccag
    21421 ggggaacagc atcttcaccg gcgcttcaag cgccagcttt tcggcgcgct gaaaccgctc
    21481 aacccgacgc tgctcggact gtgcgcgcag cagaggtccg agactcatgc ccaggcggtc
    21541 cgcctgggtg atggccgtca cccactggcg caacgcggga aggtcggtgc ggcgggccat
    21601 gtcatccaaa gcctccagac gtggcatccc cgcccgcatg tcggcaagag cgtagcgcaa
    21661 ctcctggcgc agcggattgt cggggatatt cttcgcagcc agttcgagcg cgccatgtag
    21721 attcaagccc gcttcgacgc aaagcgtcgt catatcgacc aagaagggaa attcacgcaa
    21781 catttcccgc cgccggcgat ccatgcgttc acgcaacgcg agccgtggcc acaaaccgca
    21841 catcaagcca agcagagcgg atggatacac cgcagccata gggttggtga tggggccaca
    21901 gaaaaatagc gtcccccacc cggcaagaga acccgtcaag gcggcaaggc actgcgccgc
    21961 gacgatacgt tcgggaggcc acaccactgg atccagcccg gccagacgga ccgctcgctc
    22021 aagcgtctga cgcaggcgcc acggcaaaaa cgccttgcac accaacgaca aggcgagaat
    22081 ccatggccag gccattctgg gcagccaata ccgcggaccg tggcctggcc tcgaacctgt
    22141 cacagccggc ccgagacaaa acctcagcaa caggctgacg gaaacgccca tggccaggca
    22201 actcatccag aaatacatac accctccgca tgcggctagc cctgcacatg aaatggaaat
    22261 agagcaacca tctatacgtc gatatcgaca atgcgccgga tgagcaacac acccgccacc
    22321 tccagcacag ccaccgcgcc cagaaccaac cagcccgggg tcgtatgcca caacgcggac
    22381 gccgcttcgg gatccagctc gttaaggatc aaaaccatgc cgggcggcaa caaggccatg
    22441 atccaggcct gcatgcgccc ctgggatgtc agggcgcgga tgcgtcccag caattgcagg
    22501 cgcgcccgca ctgtcgcggc aatgcgttcg agcgtttcag ccagattgcc accgctttgc
    22561 atggcgatct tgagagaaga aaccaccagg cctatgcctt ctgtggtgat gcgccgatac
    22621 agccccgaca aggcatcatc gagcgcaacc cccatgcgct gctcgcgcag catcagcccg
    22681 aactcttggg acagcggttc gggagcctga ggaacgattt gtctcaacgc ggactgcatt
    22741 ccctgccccg cccgcagcgc gccggccagc gcgagcagga aatcaggaag ctgctgatcc
    22801 aggcgctcca gacgccggcg acgcagacgc ttgagcacac accatggcat gaccaacgag
    22861 aacgcgccca caagtacagc gaacgcgggc gatcccgtca cgacatagga actcaacgtc
    22921 aatccgccgc aaaccatgag attcagcacc cagagctgag atggatcgag aaacaggaag
    22981 aattcgccaa ggctcgtttt cgcccgggaa tgaaagaact cctgatagcg tgcgtagaat
    23041 cggagcaggc aacgcagcgc gaaccagaag gtcagcgcgg ccaccagcgc ggcgcaggca
    23101 acggcgatat ggctcatggc gtatgaacct gaaccaccgg cacatccttg caacgggctg
    23161 gatggcgttg acgaaagacc tccaagtcaa gctcacaggg gagctcgacg tggcccgaga
    23221 aaaaatccgg aagcatcccg cagcccgcga aaacccgcgg ctcgccaggt tcaagccgaa
    23281 acagctcctg caactgtatg cggccgcttt ctattccggt cacctccacg atggaggtga
    23341 tggcccgatg cccgtcaggg aaacgggctt gctgaacgat caggtcgatg ctcgcggcca
    23401 catgctcacg cacagccgcc aagggcaggt ccatgccggc catcaggatc atggtttcca
    23461 gcctcgagag cgcatcacgc gggctgttgg cgtgcagagt ggtcaacgac ccttcgtgac
    23521 cggtgttcat ggcggccagc atatcgaagg cctcggcgcc acggcactca ccgatcacga
    23581 tgcggtccgg ccgcatgcgc agcgcattgc gcaccagatc acggatctcg atgcggccgc
    23641 ttccctcgag attggcgggg cgggattcca ttgaaaccag atgggcgtga ttcaatcgaa
    23701 gctcggccgc atcctcgatg gtgacgatcc gctcgccgac gggaatggcg ttggacaaga
    23761 cattgagcaa ggtcgttttc cctgagcccg ttccccccga caccaggata ttcttccggc
    23821 gggcgacgca aacctcgaga aaccgctgca tggacacgtc aagcgaaccc agcgccacca
    23881 gatccgccat ccgagggcgg tgttgtggga acttgcgtat cgtgagactg gcgccacgca
    23941 gcgcaacagg aggaatcacc gcattcaccc gggagccatc gcgcaggcga gcatcgacca
    24001 tcggcgagct ttcatctatc ctgcgcccca acggcgaaac aatgcgctct atgacgccga
    24061 gcacggcttg ttcgctgctg aaccccgagt cgtgacgcac cagtgcgcca gcccgctcga
    24121 caaagatctc atcatggcga ttgaccatga tctcgctgat gccgggatcg gccagcaacg
    24181 gctccaacgg acccaaaccc accgcttcgt ccagcacctc gctcaacagc tccgtctgat
    24241 ccatggactt tggaagttcc gtgtcggaag ccagaatggc cgccagcgct tcacgagcct
    24301 gttcgcgcaa agcctcatcg ctgagcgccg cgatatcacg ccgccgaaga tcaagcgcct
    24361 cgagcaacgc accgtgcaga cgccgccgat gacgcaacct gtcgcgcaaa cccacctcga
    24421 ccgggcggga agagacttcc ggttccgtca ccgcgtcaac gttcggaacg gccgaagcgt
    24481 tgtctccggc ataacggctc gtgggcgctt gatcagattg gcgcccggat tgagcggcac
    24541 cgggcatccg cacggcgctt cgaccatcag tgtcctggcc tcccccgcca gaaggcgaca
    24601 caacacacga attcggaacg cgtgtgactc ggataaggca agggccgacc aggatctcat
    24661 ctccctcgca caaggggccg tactgtgtga tccgccggcc gttgatctgg gtgccgcaca
    24721 gggatccaaa atcctccacc atgacacctg gacccgactc atagaagcgg gcatggcgtc
    24781 gggcaacccg ccacgcacgc aggcacaccc cacaatgcgt tgcgcggccc acatcgaacg
    24841 gcagcggaat atcacgggtc tcgatgacac catcctcata actcagcact agaccaagca
    24901 agattcactc cttgaaggat tcgggctcga cccattgcga gccgacactc ccgtagggat
    24961 tccatgctcc gtcgcctgcg cccgaccgcc ttgacgagga cggtccgggt gatgtggcca
    25021 ccggcacgtt gatcagtacg ggctggggga acgcggactt cagaatggcg cggccgcgct
    25081 cgatgcgccg cgcgagatct ggttgatcgg cgggaacgac catgggggtg acaaaaatcg
    25141 ccaactcggt ctcattgcgc tgaaaccgcc gggagccaaa cagccggcct atgatcggaa
    25201 cgctccccaa tccgggaatc ttgtcttcat tgcgcgcttc ctcacgcgac agaaagcctg
    25261 cgagcaccag tgtctgacct gatcgcacgt tgaattccgt tgccgcccgc cgtgtcttga
    25321 gagcgggtcc gcctggcgcc gacatcgagg aatcgaccga actgacctcg acctcgatgc
    25381 gtgaacgcac cgcctggttg tgttcgatcc gaggggtgat gcgcagcgag acaccataag
    25441 gtttgaaggc ggtgttcgtg ttgccattgg catccactgt cgaatatgga acctcgccgc
    25501 ccgccaggaa ttccgccgtg gcgccacttc gggcgagcaa ctggggctgg gccagcacca
    25561 cggcggagcc ggtttttgtc atggcgttga ttcgtgccga caacaaggcg ttcaccccga
    25621 agtaccccaa tgcacgcgca gccgggaaag gagccatcaa cgcgtcttcg tcggcgggac
    25681 ggcctgcgag gctgcgcgca cccgcatccc agaccagacc cgcgttgaat cctccccgac
    25741 tggagggatt ccattgaacg ccgagttcac gcaaacggtt gcgtggaacc tcgatgacct
    25801 gcacatcgag cagcaccatg cggtcccacc ccacctggct ggtgaaatcg agcaattggg
    25861 ggtactgcct ggcaagcgct gaaatacgct cgcgatccgc gtcggaaatg gattccccct
    25921 cgaccagcaa cttatccccc accagggaca cccggacatt gggcagacgc tcgagtacct
    25981 tgcgcaattg actctggatt tcacgctgcc cgtccggcgc cacctcgacg agataacggt
    26041 gaagacgccc atcaccggac cagacatgaa gggagctcga tccgtcatgt ctcgcgaata
    26101 gaatcacttc cttctcgtcg gtcgtgaccg cgttgacaat ttggccgtta ccgaccgcga
    26161 cacgcgccac attgggaacg ggcagcacac ggacttcgcc aacctggagc ttgaggcgcg
    26221 tttcagactg tccggtggct atcccgtgaa acaccaggaa cggcacgcac atcagcaaca
    26281 agcacgcgcg gcgaggcctt gccaactgcc gggaaacctc gaccccgggc atccaagcgc
    26341 cggactccgc cagactgcgc accctcatgg cctggctccg gatccggatt gcaacaaaac
    26401 cggatcctcc ccatcggccc ctcgttccga tcgcagccag gcactcacaa gttcgggcat
    26461 gtcgggaaga tcgaatatcg gcgggaccct cggcgatttg ggcgttgccg gactcaatcc
    26521 aggcagccgc cgggagccgc gatcaccata aatgacagga atggtcgtcc gcccggcggg
    26581 cgcgggcacg gccacgccaa gcaagccggc cagatcaccg cgaaccgctt tctgcgtggc
    26641 gcctccgtcg gcgggatgcc gcaaaagcgc ggtcagggtg ccactgtggc gggcggagac
    26701 cagtttgatg gcgtcctcgg gagacacatc gagcgtaacg gatgaatacg acgcacccga
    26761 catggcgggg cgatgatcgg cgacgggttt cgcattgacg ccggtggcca gaacgagcac
    26821 accttgcagc aacggggcgg tgatgcgctt gcggtgataa tcgaatgaca cgtacagatc
    26881 gatcaagtcg ccgggttcca acagaccgga gaccgagttg atgacgtcaa cgggaatggt
    26941 catggctcgt cgcccctttt cgaggcggtt ggagaacgga ctggcctggg cgtgtgcggc
    27001 gtgcgccggc aggatagaat ctcccgcacg caacgccgac cgtagaatgg atccggaaat
    27061 ctcggtgaaa cgctcaggcg cgagactggt gcttgacacc atggaagcgg gaaattcacg
    27121 gacggcgaca ttgtccggac caagccgcgt gcctgcggga agatcatggg cggccacgac
    27181 acgtcgagcc atgggtatct tcgcctggtc ctcgatttgc cgcacgcgcg accgcaaata
    27241 ctgttgcatg ctcagcgcgg ccactcctcc cgcacacacg gccaaaacca gcatgatgag
    27301 ggatcgactg gacatgatcg accgcatcgg tgtctccttt gggtgttaac actaaccacg
    27361 gaattggacg aacaacgcgc ttccggaaac gtctggcgtg atctcgaaca tcaaacgcga
    27421 ccgctcccgc gcccaaacct ccatctgtcc ggactggccg ccgggctcac ccggcatacg
    27481 cgaccagcca aggcccacaa gccgctccct gacggcattc ccggcggcgg cagggggctg
    27541 cgacaggcgg tggatgcttt gacgcactgt cattccggcc tgggtcagtg tctggtcgaa
    27601 atacaatccg gacccctccg gcagccagtc gggagcggcc cccacggccg gcgtcggcga
    27661 acccttgtcg gccagtggat tcaaaggcat ggcggagacc aagccttgcg ccccgctcga
    27721 ctcgggtccg atgtcggcca cccaatgcca accattgcgc gcaccggaca gcactacccg
    27781 gtccttgaaa gccagaaccc ggtcgaacat accggactca cgcgcgaacg cctcggccgt
    27841 gccatacaca gacatccgtg aaaagaacgt ccggtagtgc acggacacgc ctccgagtgc
    27901 cattgtcgtc atgtcctgcc acgaaacacg tacttgtgtt ttcaacacac taaagcccga
    27961 gccgaccggg tcaccgtccg cagcgggtgc cgcagtggcg gaaagcagga acagcagcag
    28021 gcacgcgacc cgcctggccg aaggaacgcc cgaaagaatc ccgcacgctt tcatggcgcg
    28081 tctccggttc cgcccaggtg atacgcaggc agccgcccag cccagggttc gagccaatcg
    28141 aacagcggcg catcccgccc ccagctctcg tcgacagggc ctgagacaat ggctgtgcgc
    28201 cgcccaagcg aataggatgt ttgagccata tcggcccacc cgggtcccga ccgggccacc
    28261 cgccgttgcg ccgagacatc gtcggaggcg tgcgccgcgt ccaccagaat ggcgacgcca
    28321 cgatccaacg atgggtatgg cgctagccag gcgggcctga ccctcccggg gagggccatc
    28381 tggtttgaaa tggaaacacc ggtctgagcc atcaggatgc cccattcatc gatccgcaga
    28441 ccatgccgca acgcggacgc ttcctggtca acaccacccg gatgcgcggc cacatccagg
    28501 tcggacagcg ccgataccga taatcgcact tgcgatccat cggagatgag ccgcgtgcca
    28561 ttcgaccggg cccactgatg cgaagggccg gaaaagaagt gacggataat ctgcgacaac
    28621 gggcgcagag ctgggtcgcg ggtaagcgaa aacgcggcga atcggctggc gtgggccgac
    28681 tgcaaggcaa tatcctgtag ccggccgagc caagggatga ccatgaatat tccccccagc
    28741 gcgagcatga tcaccaggct ttcgaggagg gcttgccccg cgcatgcatg gctggaaccg
    28801 gatggcctca tggttgttct ccggatcgag gcgcaacgag tcgagcctgc cagtaaggat
    28861 gaaacaggtt ggcctgttca agccggccgt cggctctcgc ggttggccga gcgaagaacg
    28921 tctcggcggc gctgcgtgtc accaatccca ggcctcgagg ccccggatgg cgcaatgtga
    28981 cgtcgaaacg aagcgcttgc ctgtcttctt caagatccag atatgatggc aagccggaac
    29041 cccgccagcg atgtgcattg gccaccgctc gggaatccgc caacggattg gaatcccctg
    29101 caatcaggtc ccagtcggtg gcctgacgaa cccaccgcca gaaatcctgc gaagagaagt
    29161 cgtcgggtgg agtatcgata taaggcgaac ccagggtctg cccggcggca ctcggtatcc
    29221 agccccaccc catcggatac tcacgcctgt agcaacctat ccagcgattg gcccggagcg
    29281 catgaaaaga ctgggtgtcg atggattgcc agcggccgct ggaatcgagt tcggtgacgc
    29341 cgcgccgccg caactcatgg cgcaatccgg ggcagcgcgc atccaccatc catgggttga
    29401 cggccgtatg gttgcggggc cccaggaaac ggtacaactc ggcaacttgc tggatgagcg
    29461 ggcggagatc acgaccggcg gaacgcgtct ggagaaaccc ctgccagttg tcgcccgtga
    29521 tcgtcacatc atacgtgcca tccacacccg gctccgtgtg acgcagcccg ttttgcctga
    29581 gcacagcctg aatgacctgc catcgagccg cgggaaggct gtttgccacg gaccgctgca
    29641 catgaccaaa gacacgcatg acgaaatcat catgtgccgc gtatgctcgg gccagttcgc
    29701 catccgtgtc cgccagggaa gccagcccca acgcgccacg agaggccgca tacgccgccc
    29761 catgggcggc gccgaacaac atggcgacaa ggggcgccgg aggattgccg atgcccgcct
    29821 ggcgtgcctg ggtggtgctg aaagccgacc aggacccgag cgtcaccagg tgtgccatgg
    29881 cgatctggtg gccaatctgc gcggtattca ggtaagccag catattcagc gcgcgcgcct
    29941 ggacaagcgc gccgctatag gctgcggcat cgagcgcatg cacttgcccc gaccgcgcgc
    30001 cgacgacttg cgcggtcgag aaataccgca cgaaggcaat ggacgccaca cccgccagca
    30061 acatgccaag caccaatgcc tgcccgcgtt gcgactgttt gtcgggcttc attgcccgcc
    30121 tccggcgttt ccggtgaacg atttcaaact gcgcgccgcg gtttgggtgg cggccgcatc
    30181 agccgccgat tgagcggcgc gcgactgttc cgacccatct tcaccggcga gctcccgggc
    30241 catcgcggcc gtctgggacc gcacaacctg gccaaaagac tggtagaccg ctatggccgc
    30301 cacggcaacc aacgcgacga tgacaatgta ttcggtcata ccttggccgc cttccctggc
    30361 gcggatattc tgtttcgcca cccgccgcat agccaccccc gaccattcaa acccatgttg
    30421 gatcacagtg tggccggcaa cccccggtgc cgcaatccgc agataccgac ctgggcaaat
    30481 cgcgaacagc gcgatcccat gtatccactt cgccacggtt cggccggctt ccatccgccg
    30541 cgatcacacg attgtcgaca gcgccacgcc cttctgaaat aatcgatcgg ttgatccacc
    30601 cccgggcccc gcatgacggg tgcctggtgg acgacatcaa cacatggttc acaaaatcaa
    30661 attcaaccag gagacaagat gagttccatt cccacacagc cgggggcggc gttcgacgac
    30721 gccccgtcgg cactcgagaa agcggcctat cgcaaggtaa gctggcggct gattccgttc
    30781 ctgctggtgt gctacatcgt ggcctacctg gaccgggtca acgtgggctt cgccaaactg
    30841 cagatgctca gcgacctgca catgagcaac gcggtctacg gcctgggcgc gggcatcttc
    30901 ttcctgggct actttctctt cgaagtgccc agcaacgtga tcctgcacaa ggtcggcgcg
    30961 cgcatctgga tcgcccgcat catgatctcc tggggcatca tctcgggcct gaccatgttc
    31021 gtgaccacgc cgaccatgtt ctacgtcatg cgcttcctgc tgggcgtggc ggaggcgggg
    31081 ttcttccccg gcatcatcct gtacctgacc tactggtatc cggccgaacg gcgcggcacc
    31141 atgaccgcct ggttcttgtc ggccgtggcc ctgtccggct tcatcggcag cccgctgtcg
    31201 ggctggatcc tggaagacct cagcggcgcg catggccttg ccggctggca gtggatgttc
    31261 ctgctggaag ccatcccgtc catcgtcatc ggcttcatca cgttcttcta tcttgacgac
    31321 cgcatccacc atgccaagtg gctgagccag gaagagaaaa ccctgctcga gcgcaacatc
    31381 gaggccgata ccgccgacaa gcacgatctg cccctggccc aggtcttcag caacccccgc
    31441 atgtgggcca tgagcctgat ttatttctcg ttcgtcatcg gcctgtatgg cgtcagcttc
    31501 tggctgccca ccatcgtcaa atcgaccggc atcaccgatt cgctgagcat cggcctgatc
    31561 accgccattc cctatggctt cgccgtagtg gccatgatcc tggtgggcct gcgctccgac
    31621 aaacatcgcg agcgccgctg gcatatcgcc attccggcgg tcatcggggc ggccgggctg
    31681 tacctgagca cggtctgggc gcaccagacg acgccggcca tcatcgccct gaccttcgcc
    31741 ctcatgggca tcatggtcgt cctgccgctg ttctggagcc tgcccaccgc cattctgggc
    31801 ggcaccgccg cggccgcggg catcgccatg atcaactccc tggggaatct ggcggggttc
    31861 ttcggaccca cgctgatcgg ctacctgaaa acgaccaccc attccaccaa tagcggcatc
    31921 tatgtgttgg cggcgttcat ggtgctgggc gcgctcatca cgctggccat gccgtcgaaa
    31981 ctggtcgaca aataacgcgc ccgagacgac ccattcaatg cggtgcacgt gcgccgcgaa
    32041 gcaaacaaag cggccccgtc gagttcagac ggggccgctt tgctttgggt cagtgcgttg
    32101 ggtcagtacc cttggcccga accgggatac cgcgcgacca ggtgcgccct cagcaccggc
    32161 cccgagaagg ccagggttgg atcagtgcac ccggaccttc agttcgccca gaccttcgac
    32221 tgcgccgacc atggtgtcgc ccgcgaccac gggaccgacg ccttcaggcg tgccggtgaa
    32281 caggatgtcg cccgccttca gttcgaacag gccggacaga aaagcgatgg attcggccac
    32341 agtccagatc aggtcggaca gatcgccgga ctgtttgcgg gtgccattga cgctcaaggt
    32401 gatggcgccg gatgcgagcg tgccggtttt ttccagagga tgaaccgggc cgatgggcgc
    32461 cgagcagtcg aagcctttgc cgacctccca ggggcggccc tgcttcttcg ccgccccctg
    32521 caggtcgcgg cgcgtcatgt ccaggccgat ggcgtaaccc cagacacatt ccgccgcttg
    32581 ctcgacggtg aggtcacgcc cgcctttgtg cagcaccacg accagctcta tttcgtattg
    32641 caggtcggcg gtcttgggcg ggtacgggat ggagccggtc tggccgtcgg caacattgac
    32701 gatggcatcg gccggcttgc aaaagaaaaa cgggtcttcc cggccggtga accccatttc
    32761 gcgcgcatgc gccgcataat tgcggcccac gcaatacacg cgccgcaccg ggaaacgggc
    32821 tgcggagcca acgacggcca gcgttgcggc gggttcgaca ggaacgatgg tatccatggt
    32881 ttatttctcc ttgcgtgctt tcttgtagcg ttccacgcca tcgaggatct ctttgtgtgc
    32941 cgcctcgaca ccctcccagg ccttgacctt cacccacttg cccttttcca gatctttgta
    33001 gtgctcgaag aagtgctgga tgcgggccgt ttcagcgggc aggtcttcag gcttgctgat
    33061 gtggctgtac ggggggtaca gcttgtcgac cggcacggcc agcaatttgg catcgccacc
    33121 cgcctcgtcg tccatctgca ggacacccac cgcgcgtgcg cggatcactg cgccggaatt
    33181 gacggggaat ggcgtgagga ccagaacatc gaccggatca ccatcatccg agatggtttc
    33241 cgggatatag ccgtaattgc acgggtagtg catggcggtg agcatgaaac ggtcgacgaa
    33301 caccgcgccg gaggccttgt cgacctcata tttgacaggc accgaattca tggggatttc
    33361 gatgacgaca ttgaactctt ggggcagctt ggccccggcg ggtacgcgat ctaggctcat
    33421 ggtgtgggac tctgggttga gaaaaggatt cagcgccggc cggaatggcc gcttggtcgg
    33481 tcggaggtct gcgacaggtc gacccccggt atgggcgcgc tgtcgaagta gtcgtccagc
    33541 gcggcgtcgg cgggattggc ctgcgtggcg gacaccggca cgacggcctg ggattccgcc
    33601 gagtcggcgt cgaccaggta atcaaccgca ctggcggcca tcgggacgcc atggggagtg
    33661 acgggctcag ccgcgcgcgc aacgtctatc ggcgccggat cactgcgcat atcggccgag
    33721 ggcagtgcct ggttcgtcgc aagcaccggc agcgtgccag ccacgcccga cgccaggcgc
    33781 caatgtttca tcgcatctgg ctggcgaccc aggcggtcgt agaggttgcc caggagcgcg
    33841 tgaatacgca cgtcgccgcg gatcttcatg ctgcgcagaa gatagtgttc gccctgcccc
    33901 cagagctgcc cgatcaggca caagttgccc aacgccgcga gcaggtcggg gtcttcggag
    33961 cgtgacttca gccagacttc ggccttgccc aaccggcgcg ccacctgatc cggcggacac
    34021 cgaccgtatg cattgagcag gcgcggatcg gcattcacgg cgatggccgc ttcgagtatc
    34081 ttcgcggcct cgtcgtggtt gccctgggta tcctgcacac atgccgcggc cagggccaca
    34141 tcgggcaatg tgcgctcatc ggcgcgcaag tccgaccaga tagccttgaa ggcatccatg
    34201 ccgccggccc gcaggcgcgc cgcggccgcc gtttcaatga tgggcatggc ttgcggtttg
    34261 tcgagcacac cacggcgcag gagcagccgg gtgaggtcgt agacacgatc gtgattgttc
    34321 aactgcctgt gcgcgcgaag cagcaggcgc gtggcgttga aatagcgtga actggcgtcc
    34381 tgcagcggct gcaagagcac cagcgcgtcc tgcgggcgat tctgatccag atacatctcg
    34441 gcggcgacag tggcagccgc gtctttgagc cggggttcgg tgccggcctg ttcgcgggcg
    34501 aacttcaacg cctcgtcgcg ccgggaaaac tcacccagat aatgggaggc gcgcgccgac
    34561 gccaggcccg cgagcacctt gcgtggctgc gaacgggtat tccccatgac tttggccagt
    34621 tctttctcgc cttgcgtgaa acgcccttcc agaacattga gccaaccact ttcgagcagt
    34681 tcgtggtcgc gcttctcgcc gcgccagccg cgccacgcgc ggaaccgggc cggccccgaa
    34741 accagccatg ccagaaaccg gatgacaaca tataaaacga agaacccggc gatgagcagc
    34801 agcacagcca gcgtgaggga aagctcgatg cgccacggct gggccacgat gagcacgttc
    34861 ccgctgtgtt cgcgcagcac cagccccagg gcaaccgcca ggacgaacac caggatgatc
    34921 cagagccatg tcctcatcgc ttattcctgt ggcttcactg atgaggccgc gcttgcggcg
    34981 gacccggatg acgcattgcc gctcgaatgc gtgctcatcg tggaggccgc atcggcggac
    35041 ggcatcgctt gaccgcgacg ggattccgcc tcgcgcagcg cctcgatagc ctgaaggctg
    35101 ttctccacgc cgggcggttt ggccgcgacc tgggtccggg caaggctcgc gaccagttcg
    35161 agcgccttgc gggtctgggg atccttcgta tcgaagcgcg tattcaatgc ctgttccagc
    35221 gcctgggttt cggcattcca gactgactgc tgacgcatca tgagggccag ttgcgcggtc
    35281 atgatgcgca tgtgcaagtt ctcgcgcaac tgatgcgctt gatccggtga catcagcaac
    35341 gccgccggat cgcttacgcg ccggatgctg accaggtgcg cgagctcgtt gctcgtggag
    35401 gaccatgctg aatgccacca gccccgcacc acattcaggg catggcgcca gcccgtgccg
    35461 cccgcgccgg gctctgcggg agtgggtggc gcgtcggcgg aagacgacac gcttgaagcc
    35521 aatcgcggcg cggccgcgtc gggtatcaac aacgccgcat gaccgacgag gccatcaagc
    35581 tgcctgagct gcgtggatac ggcggcgaca tcgaccactg acgcggcgcg caggcggtcg
    35641 agatccccgt tgagggtttg ctggagtgat gccagagcgg gccgattggc gcgcgccagc
    35701 cgagactggg cggattcgag cgcgatgatg gcattggcga cattgccatt gagcgtcagc
    35761 tgctgattgg cgatggtgac catgtgatcg acatcgttca ccagcgcgag atcggagcca
    35821 ctgtcggtca agttctgcag cgcctggccg agcgcatgga cctgttcctg cgcatcttgc
    35881 agcgacgcct gcagggccga gacccgggaa gcctgcaatc tcaccatgga aatcgcctgt
    35941 tgcgcctggg cggacgcctg gctggccgct tgcgcgctgc tttgcgcctg gctggacagc
    36001 aatgcggagg tctggtggaa ctgacgttgc tgataccaca gcgcgatggc cagcgccacg
    36061 gcaataagca ccacgatgac gagcgccacc caccagccac cgcgcttctt cttcacggcg
    36121 gagccgccgc tcttggtcga tgatggcgat gccgccgagg ttttctgggc ggatgcggac
    36181 ggctgcgcgg tttgcggagt ctcggttctg gattctgtca tagcagggat tgtaaacgct
    36241 cgtcggccgg aatcatgata aagccagcga ctggatggct tggaacactg cgccgtcatc
    36301 cgcggcgcaa agccgcacgg acggcggcac gccagaggca accgtgaagc gcgacgtcag
    36361 gcggctggct acgcgtgggt gaattgtgac aaatcggacg cgttcccaca actggcgcaa
    36421 ccggaccgca tgatgctcga tgttcgcatg cagcgcatcg gcgctctccg tgctggtgac
    36481 caggcacacc gacgacgacg cggcggacag ccgccgcgcc agttcatcgg cctggcccgc
    36541 cgcccagagg atcggctcgc gacggtaagc cgcatggtgc cgcacttcga ttccggcgcc
    36601 ctcgagctgg cgcccgagcc actcccggcc ggtttcgccg cgcacgatga gcgcgcgctt
    36661 gatggctggc agacgcggtg cgagcagcgc ccacagcgcc tcggagtcat ggttgggtga
    36721 ttcggggccg ggatggacga tattctccgc cggaatgacg ccggcatcgc gcaacgcccg
    36781 cgcactggcc ggccccaccg tgcaggccag cgtggtgtcg ggccacgatg cgccccgccc
    36841 accgtgaatc tgttcgaagt agaaacgggc cgccgcggcg ctgacgaaca tgacgagatc
    36901 aaaaccgcca gggactggtg ccggggtggc ggccggaatc aacctcagca cgggctggac
    36961 cgatacgtcg attcccgcct ggcgcagttg gctggcgagg gagtcgttat gcccctgcgg
    37021 ccgcgtgagt atggccagcg cgccagagat gtccgccagc acgcgggcgc cctaggcctg
    37081 ggcctggccg gagacgggga tgctggccag gatgtcccgg gcgccctttc gcaggagctc
    37141 ggcggcggcc agttcaccca ggtgctgcgc ttggtcaaca ggcccggaca tttgcgcgct
    37201 caccacttgt gttccatctg ggcgggccac cagcgcccgc agtgtcaggg tgccggcctg
    37261 aacttgcgcg aacgcggcca gcggcacctg gcatgagcca cccagcacgc gggatacaac
    37321 gcgttcggcc accgcgcagg cggtggtgtc ggcgcacgcc agcggcgcga gccattccgc
    37381 gacatcgtgt cgcgtagcca gcgtttctat ggctatggcc ccctgccccg cagccggcag
    37441 actgtcttct ggtgacaggt aatcgcgtat gcggtgcgcc agccctaggc gtttcagccc
    37501 cgccgcggcc agaatgatgg ccgcgtaatc gccacgatcc agcttggcca ggcgtgtccc
    37561 gacattgccg cgcagcggtt caactcgcaa tgccggatag cgcgcacgga tttgcgcctc
    37621 acgtcgcagg ctggaggtgc cgaccaccgc gccggtgggc aattgcgcca gggaatccac
    37681 cgagttcgac accagcgcat cacgaaagtc ctcgcgcgcc agaatggccg gcaaggcgaa
    37741 cgaggcaggc atgtcgacgg gaacgtcctt cagggagtgg gccgcgagat cggcccggcc
    37801 atcgagcaac gcggtttcca gctctttgac gaacaagcct ttgccaccca ccttcgacag
    37861 ggtgcgatca aggatctgat cgccgcgtgt ggtaagcgtc agcaggccga tgtcgcagga
    37921 ggggtacaac gcggacaacc gttcgcggac atgttcggcc tgccagagcg caagctggct
    37981 tgcgcgcgtg gcgatgatca gtttttgcgg gccggacggg gtcatcccgc caacctgttg
    38041 acgatgaccg cggcgatcac accgatgatg gccagaatca acaggccctg gagcagggac
    38101 agccccgatt ttttcttgtc cgagggttta aacagaccca tgtgcgtttt ccttcacgac
    38161 attcttgcgg ctggcaagga accagcccac ggcgacgacg atgacggcgc cgatgatttc
    38221 cgcgaggatt ttaaccgttg tggatggaat tccgatctta tccgccacaa agacgtcggt
    38281 catgagcata ccgcccgcga tccaacccag caacgcggcc cccagcgtca ccacggcggg
    38341 gaagcggtcg atcagcttga gcaccagggt gctgccccac acgatgatgg gaatgctcag
    38401 caacagaccg aacacgacgt aaagcagctg atggtgctcc gcggtgtttt gcgcggcccc
    38461 ggcgatggcg ataacgttgt ccaggctcat gacgaaatcc gccacgatga tggtgcgcac
    38521 cgcccgccag atggtgcccg ccgcggccac gtcttgatgg ccttcttcgg gcagcatgag
    38581 cttcacgcct atccacagaa gcagtatcgc gcccacgatc ttcaggaaag gcaccgccag
    38641 caagaacaac gcgaaggcga tcaacaccac gcgcaggacg atggcgcccg ccgtgcccca
    38701 gatgatgcca cgcacccgtt ggcgggcatc cagattgcgg cacgccagcg cgatgaccac
    38761 ggcgttgtcg ccccccagca gaatatcgat gacgacgatc tgggcgatgg cgccccaatg
    38821 caatgcctga atgaattcgc tcattgagct cccttttctc ttgaggatac cgggggccgg
    38881 aatgaacgga tatccgattc aggaccctgc gattcgctat ggctggcctg ttcgaatgcg
    38941 gaacaatcag gcctaccggc tgattggcgc atgagccggt caaccgtatg attgtaattc
    39001 agctttttgt cagtttatga atccctggca cgcgataagc ggagtcggcc aaggccaagg
    39061 acgatcagtg cgcccagaat gcccgcgaac agcggcagca cgtcggatgc cagcgacgcg
    39121 gcacggtcga tacgcgtcat cagtggtgtg acggctggat cgccaaccag catttggccg
    39181 gcgatgaaac ccagtagcgc agccccaagc cagacaatgg cggggaaccg ctcgatcact
    39241 ttcaacaaca aggtgctgcc gaacacgacc agtggaatgc tcagggcgag gccgatgatc
    39301 agtagcgtca ggctgccctg cgccgccgcg gcgacagcca ccacgttatc caggctcatg
    39361 atcacgtcgg cgatgacgat ggcgcgtacc gccacgccta ctccgcttcg acggttccga
    39421 ggcctggtaa caggcttggc cggcgccagc aggcccacgc cgatgaaaac cagcagcacg
    39481 gcgccaacga tcttcagcca tggcaacagc aacagccgcg ccgccagcag ggtcaggacg
    39541 atgcgcaaca gaacagcgac cgaagcgcca ccgcccaccg ccaagcgttg ccgcgcgggc
    39601 ggtagattgc gcgcggcgag cgcgatcacg accgcgttgt cgccggaaag caggacattg
    39661 acccagatga cttccagcaa tccgaaccag aaatccgcgg aatgcgtcat cattggccgc
    39721 ccgtgtcgga ttcagcccac gctttccagg cccagcctga cacacctcgt cgcgaccggg
    39781 cggcggctgg ccgcccggct ttcagggtaa cgccacgagg cagagcgacc cgccccgtgt
    39841 cagagaacgg tcttgagcag cttgcccatt tccgacggat tgcgcgtcgt gcggatcccg
    39901 caggcttcca tgatctcgag cttggcatcc gccgtgtcgg ccccgccgga gatcaaggca
    39961 cccgcgtgtc ccatgcgttt cccgggaggt gcggtgacgc cagcgatgaa gcccacgatg
    40021 ggtttcttca tgttgtcttt cgcccactca gcggcattga cttcgtcagg cccgccaatt
    40081 tcgccgatca tgatgacggc gtcggtgtcc ggatcgtcat tgaacatctt gaggacgtcg
    40141 atgtgcttga gaccgttgat cgggtcgcca ccgataccca ccgcgctgta ctgcccgaga
    40201 ccgagctcgg tgacctggcc cacggcctca taagtgagcg tgccggagcg gctgaccacg
    40261 ccgatgcggc ccttgcggtg aatatgtccc ggcatgatgc cgatcttgat ttcgtccgga
    40321 gtgatgaggc ccgggcagtt gggcccgagc agcagcgttt tcttgttctg gtcgcgcatg
    40381 cggttgcgca cctcgagcat gtcgcgcacg ggaatgcctt cggtgatgca gatggccagg
    40441 tccagatcag cctcgaccgc ttcccagatg gcggcggcgg cccccgcggg cggaacgtag
    40501 atgaccgaaa ccgtggcgcc tgtgcgctct ttggcttcct tgaccgagcc gaagatgggg
    40561 atgccttcga agtcttcacc ggccttcttg gggtgcacgc cggcgacgaa cgcggccttg
    40621 ccgttggcgt agtcgcggca catgcgcgtg tggaactggc ccgtcttgcc ggtgatgccc
    40681 tgcgtgatga ctttcgtgtc tttgttgatc agaatcgaca tatggaaatc cttgacaata
    40741 ttcttatttc gcggcggcga cggctttggt ggcggcctcg gccatggtgt cggcgctgat
    40801 gatgggcagg cccgattcgg ccagcatctt cttgccgagc tcttcgttgg tgccccgcat
    40861 gcgcacgacc agcggcacac tcaggttgac ggccttgcat gcggcgatca cgccctgggc
    40921 gatgacgtcg cagcgcatga tgccgccgaa gatgttcacc aggatggcct tgacgccctt
    40981 gttcttgagc atgatcttga aggcttccgt gaccttctcg gcggtcgcgc caccgcccac
    41041 gtcgaggaag ttggccggct cgccgccgaa cagcttgatg gtgtccatgg tggccatggc
    41101 caggccggcg ccgttgacca ggcaaccgat gttgccgtcg agctggatat aggccaggtc
    41161 gaacttgctg gcttcgattt cggccgggtc ttcttcttca agatcgcggt aggcgacgat
    41221 ctcggggtgg cggaacagcg cgttggagtc gaagttgaac ttcgcgtcga gggcgatgat
    41281 gtcgcccgag ccagtcagga tcaatgggtt gatttcagcc agcgaggcgt ctgtttccca
    41341 gtaggtttta tacagcttct ggaattcgac ggcggccttg gccaccgatg tctcgggcac
    41401 gccgatgccg cgcgcaagct tctgcgcctg ctggtcggtc aggccatggg tcgggtcgac
    41461 gaattccttc aggatttttt cgggcgaatg cgccgcgact tcctcgattt ccatgccgcc
    41521 ttcgctggag gccatgacac acacgcgctg cgaggcccgg tcggtcacga taccgacgta
    41581 atattctttc ttgatgtcgg cgccctcttc gatgagcagg cggcgcacct tctggccggc
    41641 cgggccggtc tggtgcgtca ccaactgcat gcccaggatc tcggaagaga gttgacgcac
    41701 ttcgtcgagc gaacgggcca gcttgacgcc accgcccttg ccacggcccc ccgcgtgaat
    41761 ctgcgccttg acgacccaga ccgggccgcc cagtttttcg gcggccgcca tcgcttcatc
    41821 aaccgaaaac gctggaaatc cgcgcgggac cgtaacaccg aattgcctca gcagttcttt
    41881 gccttgatat tcgtgaattt tcatttatta cctgtcagaa cgtgaaaaag gggaaaacag
    41941 gagtccgggc gtggacagcc cgcgccgccg gaaaccggcg ccacgactac tcgcgcctgg
    42001 cctgccgggg agagacatac cactgcgggt agaattcttt cgccaccggc ccgtcgacgc
    42061 gcaaagcgtg acaaccatcg agctggaacg ggcgtttcgc gcctggctgg gcgggttgcg
    42121 tagccgccag acggcgctgg cgggccatgg actggatcgc cacggtgggc agaacataag
    42181 acaattcagt gaggtgcgtg caacccgccg tgcgaccaaa gcgttctttc accgtctggc
    42241 ggaaattgcg caacaggttc atgccgacca gatcgttgta atcaggcgcg atggcggtgc
    42301 aatgcggccc gtaaggtgcc gcatcataag cggcggtcgc cgcggtgatg gagaaatcgt
    42361 cgttgatggt gacgcgcaaa tgcatgtggt gcaccgggtc gcccgcccga tgcgtggaac
    42421 catcggcccc ggggaagtcg taggccttgg tgtcgatgag ttctgcttcg agatcccact
    42481 gcccgtcgtc ccgggcaaac gcctgcacgc gtatggaccg cgtatgcagt ggttcacgag
    42541 aagtatcagg gggcggcaag ggcatgtgcg ggggaaaagc gagcaacttg ggcgctaaaa
    42601 cccatcaagt atagcgcacg cggcgcgggc ccgtgcaccg cccaggccgg tttccccagc
    42661 ctggcatccc ccgccgcgtt ccaggcaaaa cccgtccaga gggaaccggg tcaccgggaa
    42721 accccggctc ccggcaatcc gtttggacga cttggcccgg cgcgtgaatc aggccgcctc
    42781 gcgcagcacc ttggcggcgt cgaccatcgc gaccagggcc gcctcggttt ccggccaggc
    42841 acgggtcttc agcccacaat ccggattgac ccacaggcgc tccggcggca gacgccccgc
    42901 cgccttgcgg atcagatcga ccatccagtc cacattgggc acgttgggag agtgaatatc
    42961 gtagacgcct ggcccgatat cgttgggata acggaaatcc tcgaacgcgc ccagcaactc
    43021 catgttcgag cgtgacgtct cgatcgtgat gacgtcggca tccatggccg cgatggcctc
    43081 gatgatgtcg ttgaattccg aatagcacat atgcgtgtgg atctgcgtgt cgtccctgac
    43141 tcccgatgtc gaaagacgga agcaatccac cgcccaggcc aggtacgtcg cccagtcacc
    43201 gcgccgcagc ggcagtccct cgcggaacgc cggttcatcg atctggatga cgcggatgcc
    43261 ggcggactcc agatccacca cctcatcgcg caacgccaga gccagctgcg cgcaggtggc
    43321 ctgacggggt tggtcatccc gcacgaacga ccattggagg atcgtcaccg gcccggtgag
    43381 catgcccttc acgggtttgt cggtcagcga ctgggcgtat gccgtccagg ccactgtcat
    43441 gggggccggc cgcgacacat ccccgaaaat gatgggcggc ttgacgcagc gcgagccgta
    43501 gctttgcacc cagccattgc gggtgaacgc gaaacccgcc agcaactcgc cgaagtattc
    43561 gaccatgtcg ttgcgctcgg gctcgccatg caccagcacg tccagcccga ttttttcctg
    43621 gaagcggatg acatgctcga tttcagcgcg gatggctttc tcatagccgg catcggtcag
    43681 cgagccggcc ttccaatcgc gacgcaaggc acggatctca ttggtctggg gaaacgagcc
    43741 gatcgtggtg gtgggatagg tcggcaggcc aagcgcctgg tgctgcgatt cgatgcgtcg
    43801 ctcgaatggc gcccgttccc gacccaccgc cgccgcgtcc cgcgcgcgat tccgcaccac
    43861 cgtgttgtgt gtgcggggcg agacgcgccg agcctcgagc gcggcgcgct gcgccgccag
    43921 ggcggcctga acctcggagc tcgcagtgcc atccagcgcg gcggccagcc aggccagttc
    43981 atcgagcttc tggaccgcga acgacagcca accgcgcagt tcggcatcca gctcggtttc
    44041 accctcgagc gtgaccggaa catgctgcag cgagcaggac ggcgccaacc acagacggtc
    44101 cccaagccgc gctttggccg gcgccaacac ggccagcgcc cggtcgaggt cggtgcgcca
    44161 gatgttgcga ccgtcgatga tccccgcgga cagcacttga tcggaccgca acactgccag
    44221 cacgtcgtcc agttggtccg gagcgcggac cagatccacg tgcagcccct gcaccggcaa
    44281 atccttcagc acggacagat tgtcgcccaa cgcgccgaaa taggttgcca acaggatgtc
    44341 cagcccggaa ccggccaact tgccgtagac ccgggcgaaa gcctgtttcc agtcatctgg
    44401 caagtcaagc accaggatgg gctcgtcgat ctgcacccac tgcacaccca tgtctttcaa
    44461 gcgccgcaga atctgcccat acaccggcag cagattcgcc agcaacgcca gcttgcgcgc
    44521 atcacccggg ccgtcggaga acgcatcgcc cttgcccagc catagccagg tcagcgggcc
    44581 gggaatcact ggcttgacct gcaggccctg tgcctgagcc tgggccacct gctcgaatag
    44641 tgtctcgcgc gcgatacggt aggcctgtcc cggcaccagt tcgggcacga tgtagtggta
    44701 attggtgtcg aaccacttcg tcatctcgca ggccgcggcg ggcctaccgg tgggtgcccg
    44761 cccgcgggcc atgcggaaca acgtgtccag gccgacggac tcgtcgtggg gttggccgaa
    44821 acgtgccggc accgcaccca gcaacgtggt ccattcaaga atctggtcgt accaggcgaa
    44881 atcgcccgcc gggacgaatg ccatgcccgt gtcagcctgc cgcgcccaat ggcgggcccg
    44941 cagatcgcgc cccgtgtcga gcaacgcatc gacgtcctgg cgaccggccc aatacgcctc
    45001 gagggcgcgt ttcagttcac gctgggcacc gatgcgtggg aaaccaagat tatgaataac
    45061 agtcatttta ttttcgaatc acagagattg atcacaacgc cgtccattgt gccccggttc
    45121 attcatgata aaaaatcaaa ttgaacattc ataaaatgag aatttttcat ataattatgg
    45181 aaatatgatt tttcctgtcc gcggatcaaa actcgtctct ccgcactgcc tcgcatctgc
    45241 caccccatgc tcgaaatccg acacctggaa accctcaccg ccatacgtga aaccggcagc
    45301 ctgcaagagg ccgccgagca gctgcacgtc acccagtcgg cgctctcgca tcaattgcgc
    45361 gacctggagg tgcggctcaa gacgcccttg ctgaatcgcc gcacccggcc cgcccgcctg
    45421 acaaccgccg ccctgcgtat cctcacgctg gctgatgacg tcctgccccg gctgagggcg
    45481 accgagcatg aaatccagcg cctggcggcc ggccgcaccg ggcgcctgca cttggccatt
    45541 gaatgccatt cctgcttcca atggctgatg ccggcgctgg acgcattcag gcagggctgg
    45601 cccgatgtgg cccttgatct ctcggccgcg ttctcatccg cgccgcagtc ggcattgctg
    45661 cgaggcgacc tggatgtcgt catcacgtcc gacccgcaga cgacggaagc ggtggagtac
    45721 tcgccgctgt tcagctacga actcgtgctg gcggtatcgt ccaccagtcc actggcccgc
    45781 catgcctaca tagaaccaga acaactcgct gaccaagtgc tgatcacata ccccgtggac
    45841 aagcaacggc tggatgtcta tacggcattc ctggatccgg cgggggtaga gccagcggcg
    45901 atacgcaagg ctgagttgac cccgatgatc gtgcaactgg tggccagcca tcgcggggtg
    45961 gccgccctgc ccaactggac actgaccgaa tacctgggac agggctggct gcgcatctgc
    46021 cgcctgggca agaccggtgt ttggcgcacc ttgtacgcgg cggtgcgcgt ggaagacgcc
    46081 gatgcgccct acctgcatgc gtttctcgat caggcgcgca ccacttgctt cgccacgctg
    46141 gatggcatca agcaagctga cggctcgccc acacccggaa cactccggca acgatagccc
    46201 cgctcttgca ataccccaag caaggcgtcg ggcactccct gccgaccaca gaacaagtac
    46261 accacgcgag ccccctcgcg cacttgctcc agcgcctcca gcaacgggga ttcgtcgatg
    46321 gcggacacct ctccgctcgt ggcatggcgg gtggcgtccc ggcgcgccag atcgagcacc
    46381 gccggatcgg gaagcggttc atgcagcacc acgtcagcgg caccgagttc gcgtaatccc
    46441 ctgagtgtca gcagatccga gtctccgggc cccacatcaa cgaagcttat caacccctgg
    46501 gcctgccgct cgccacgcaa cccctcttcc agacagcggc gggcgtcttc gtatccccca
    46561 tccatcacac catccgtgcc gtgctcaacc agatgtgacg ccacaggacc atcaaacatc
    46621 cagtcataga aacggcggcg ttcgctcaca tcgggatacc gggcgcggat ccgtgtccgg
    46681 tacagccgcg ccagctccgc ctgccttgcc agggcgggat cgagcaagcc ctcgagcctc
    46741 tcgcgcagtc gacgcgccag tacgggcgcc acgcctgacg acgacacggc caccaccaac
    46801 ggcgaacgat caacgatggc gggtacgtga aagctggaaa gcgccggatc atcgaccacg
    46861 ttgacccaaa gcccgcgtcg acccgcatcc cgggccacat cggtattgat ggggcgactg
    46921 cccgtcgccg ccacgaccag acactggcct tccagccatt gatcccgata agcgccgggt
    46981 aaatgccgca ccgccccgcg ttcggccagc aaacgcagag gctcgcatag atccggcgca
    47041 ccgacatgga cacacgccct cgctgccatg agcaacgtca ccttgcgcca cgccacggaa
    47101 ccgccgccca cgaccaacac attgcggtcc tggacatcgc tgaacatcgg aaacaatctc
    47161 atgggtattc ccctggcttg cacgaaagcc ggtcgtgccc gttcgtgtcc gaccaggaca
    47221 cgccacagtc ttaaggattc tccgaacagg tcagcgcacc gcatgcatct gcgacttgtc
    47281 gccagccttg ctcaaagaat ccttaaagca aagtccgtgc ccaacaccgg gaactccgcg
    47341 ccaggcctgc cacggtctcg ccaccgccgt tgcccgcctc ggcgccatcg acctggcacg
    47401 gcttgatatt tcatgcgatt caaccccggc cccaggcggt cggcacccga acggtgcgcg
    47461 gcgggaacca tgacggggca cgcgcaccga taacgtgcac tgcgaaccgc aaacgcaccg
    47521 cggcacacgg gactgacgac ctggcacgcg gtttgcttta cctctcctca agagagggcg
    47581 tatcgcacgg catgaagaac acactggcgc cctgacctgc acactgaaca cggacccgtc
    47641 atgaagaaat tgaaactcgt agtcgtcggc aatggcatgg ccggaatcag gacgctggaa
    47701 gaactgctca agctggcccc cgacctgtac gacgtcacag tgtttggttc tgaaccacac
    47761 cccaactaca accgcatcct gttgtcgccc gtgctgacgg gcgaacagac actacaggac
    47821 atcatcctca acgatctgaa ctggtaccgc gatcatggca tcgacctgcg actgggacga
    47881 aaagtggtgg acatccatcg cgcccgcaag gaagtggtgg cggacgacgg cggcgtttgc
    47941 gcgtacgaca gactgctgct cgcgacgggt tcgaaccccg tgatcctccc cgtcgaagga
    48001 catgatctcg acggtgtcat gacgttccgg gacattcagg acgtgcatcg catggtggag
    48061 gtcgccagcc agcgacggca cgctgtggtc attggaggcg gcctgcttgg cctggaggcc
    48121 gccagcggac tggccgcgcg gggcatgcag gcctgcgtgg tgcatctggg agcgagtctg
    48181 ctcgaacggc aactcgatcc ggccgccgcg gcgttactgc gggcgaacct ggagaacagg
    48241 ggcttggaat tcctgatggc gcaccagaca gaagccatcc tcggcacccc ggacaaccgc
    48301 gtatgcggca ttcgattttc cgatggcggc gaacgccccg cggacttggt cgtcatggcg
    48361 gttggcatac gcccgaatac ggaactcgcg gaacgcgcgg ggatacacac gcaccgcggc
    48421 atcatcgtca acgacacact tcaaacctac gacccgtcca tctacgccgt gggcgaatgt
    48481 gtcaaccacc gtggcacggc ctacggtctg gtggcgccgc ttttcgagca ggcgaaggtc
    48541 gccgccaacc atcttgccct gcacggcata tcgcgctatt cgggcagcgt gacttccacg
    48601 aagctcaagg tgaccggcat cgatgtgttc tcggcgggcg atttcctggg cggcggcgat
    48661 accgaagcca tcaccctcgc cgacccgacg ggcggcgtgt acaaaaaact cgtcatacgt
    48721 gacaacaagc tcgttggcgc ctgtctgtac ggcgatacga tcgacggcgg ctggtacttt
    48781 cgcctcatca aggaagccag gcccatcgac gacatgcgtg accatctgat gctcggagag
    48841 ggttcggcgg gcgacacggg ccacgccgga cagaacagcg tggcgggcat gtcggactcc
    48901 accgaggtct gtggctgcaa cggcgtctgc aaaggcacca tcgtgaaagc gattcgcgaa
    48961 aagggactgt tcaccgtcga cgaggtcaag aaacacacca aggcggcaag ctcctgcggc
    49021 tcctgcgcgg ggttggtcga acagatactg atcgattgtg tgggcggagc ggccaacatc
    49081 aaaccccgcg cggaaaaacc cgtatgcggc tgctccgatt tcacgcatgg ggaagtgcgc
    49141 aaggcgatcc acgaccatca cctgacatcc gtgcggcaag ccatggattt cctcgaatgg
    49201 cgcaacgccg acggctgcgc gacctgtcgc cccgcgctga actactacgt gaactgctcg
    49261 tggcccggcg aggcccggga cgacgcgcag tcacgtctgg ccaacgagcg catgcatgcc
    49321 aatatccaga aggatggcac ctattccgtg gtgccgcgca tgtggggcgg tgtgaccagc
    49381 ccggccgagc tgcgccgcat cgccgacgtg gccgacaaat accaagtgcc gatggtgaag
    49441 gtcaccggcg gccagcgcat cgatctgctc ggcgtgcgca agcaggatct tccagccatc
    49501 tggaaagacc tggacatgcc gtccggccac gcctacagca agggtttgcg cacggtcaag
    49561 acctgtgtgg gcagcgaatt ctgccgcttc ggcacgcagg attcgacggg catgggcatc
    49621 gccctggaga agaatctggt cggcatgaac agtccgcaca aggtcaagct ggccgtatcg
    49681 ggctgtccgc gcaattgcgc ggaatccggc atcaaggatg tgggcgtgat cgccgtggag
    49741 tccggttggg aaatctacgt ggggggcaat ggtggcatca aaaccgaagt ggcgcaattc
    49801 ttcgtcaagg tgaaaacccc ggaagaagtg ctggagcaca ccggagcatt cctgcagctc
    49861 taccgcgagg aggcattcta tcttgaacgc acctgccatt acctggcccg cgtcgggctc
    49921 gatcacgcca aaacgaaggt gatcgaagac aacgcgaacc gggcggcgct gtttcagcgt
    49981 ctgaagttcg ccctgtcgtt tgaacaagac ccctggacac accgcgtgcg cgaacccgcc
    50041 gcgcgccgtg aatttgaaac ccttgacgcg tgagacccca catcatgaac cacgaccacg
    50101 acacagcgcc ctggactctc gcgtgccgca tcgacgacat acctcgcgcc ggatcgcgag
    50161 tcctgaaacg cgcgaacgtc gacgacattg ccatcttcag gacctgggac gaccggatct
    50221 tcgcgctcct ggatcgttgc ccacacaagg ggggcccatt gtcggcgggc ctgatacacg
    50281 gccatgccgt cacctgcccc ttgcatggct ggaccatcaa cctcgacgac ggccaggccc
    50341 aggcgccaga ccacggatgc tcgaaccgca ttccggtaaa ggtcgagcac gacgccgtct
    50401 tcctggacct ccccgccaca tgacgcgcgg cacaccacca acccccggtc atgctttgga
    50461 gcggacggtc tcgtctgtct gctgttattg cggcacgggc tgcggcgtgc tagtgcgcag
    50521 ccaggatgac caggtcctcg aggtcatcgg cgacgccagc cacccgtcaa accagggtcg
    50581 cctctgtgcc aaaggcctga atctggccgc cacggtgcgg cgcgacagca gccgcgtctt
    50641 atccgcggag ctgcgccgcc atcgtgacga agcacgtcgg cccgtcacgc ttccgcatgc
    50701 gctgtcgacg gcggcgcgca agctggcgca gactttgcgc caacacggcc cccaggccat
    50761 cggactgtat ctgtccgggc agttgctcac ggaagattat gcggtattca acaaactcgc
    50821 caggggcctg atcggcacca acaacatcga caccaattca aggctgtgca tgtcgagcgc
    50881 cgtggcggcc tataccctta ccctgggggc cgatgcgcca cccgcctgct atgacgatct
    50941 ggatcacgcc ggaacggttc tgatcgccgg ttcgaacatg gcctttaccc atccggtatt
    51001 gttccggcgc ctggaggccg ccaaggcaca caatcccgcg atgaggatcg tggtgatcga
    51061 cccccgccat accgatactt gcgacatcgc cgacctgcat ctgcccatcg tcgccggcac
    51121 cgatgtcgcc ctgctgcacg ccatgctcaa cgtgctgatc tgggagggcc ttgtcgacca
    51181 cgagtacatc gcacatcaca cagaagggtt cgagacgctg aagcagcgtg tacaggagtt
    51241 caccccgcgc acggcccagg aaatatgcgg ggttcccgcc aacgatatcg tgcgctgcgc
    51301 gcgttggttc ggatcagggg cgccggcact gtcgctctac acgatgggtc tgaaccaatc
    51361 gagcagcggc acagccaaga atgcggcgct catccatttg catctggcca cgggccagat
    51421 cggtaaaccc ggcgcgggcc cgttttcatt gaccggacag cccaacgcca tgggcggccg
    51481 cgaagccggc ggcatggcca ccctgcttcc tggccaccgc aagccgggcg acccggcgca
    51541 ccgtgccgac gtggcggcgg tctggggtgt ggacagcctg ccggaggccc ccggcctgcc
    51601 tgcggtcgaa atgttcgacg cggtgctggc cggcaccgtg aaagtgcttt ggatcgccgg
    51661 gaccaacccg gcccagtcca tgccggacca ggccaaagtg cgggcggctt tgcgcaaggc
    51721 ggagttcgtc atcgtgcagg aagcctattc gcacaccgag acgctggaat acgccgatct
    51781 ggtgctgccg gcggcgacct ggccagagaa agacggcacc atgaccaact cagagcggcg
    51841 cgtgagccgc gtgcgcgccg cgatccgtcc gcccggcgat gcccggcccg actggcaatt
    51901 ggcctgtgcg gtggctcgac gcctggcggc cgacatcgca ccggacaaag tccctctatt
    51961 ccactaccgc gatgcggccg agatcttcgc cgagcacgtt ctactgaccg cgggccgcga
    52021 cttggaccac agtgtcttga gccacgcgaa actggacgcg gaagggcccc ggcaatggcc
    52081 ttgttctcca gacgggacat cacgcccacg gctgtattcc gacggaagat ttccaaccgt
    52141 cagcgggcgc gcacacttcc tggacatcga ctacaccccc gtggcggagc ccccttccgc
    52201 ccagtaccct ttccgtctga ccaccggccg cttgcgcgac cagtggcacg ccatgagccg
    52261 cagcggtctg gtgccttccc ttacacgtca taccgaggag ccgcgtattc acttgcatcc
    52321 cgatgacatg gacaggctgc ggatcagtga cggaaaactg gtccgtgtgc gatcgcgccg
    52381 aggtgaaatc gccctgcccg cgctggcgga tacgggcctc aagcccggct gcgcctatct
    52441 gcccatgcac tggggcagcg ccttcatggc aggcgacgga atcaatgcat tgagcaccga
    52501 cgcccgtgat ccggtatcgc accagcctga actcaagcac agcgccgtgg ccattcagcc
    52561 tctggcgccc atgtgggagg cctgtgcctg ggtccgtggc gccgtgccga cgctgcggcg
    52621 ccggctcgca cggtggctgc gcgcctttcc ctacgcggtc gtcctgccca gcgccgtggg
    52681 aggggaaagc gtgcggctgc gactggccag cctcgagccg gtccccacag agatactggc
    52741 cgcattaatc gccgatttgc aactgaacga accggacatg gcttttgacg atccggcgcg
    52801 cggtatcgcg cgccgcctgc ggcgcgatga caacggtctt ctgctggcat tcctgctggc
    52861 cggcgatgtg cgggcgcagc cgcccttgtt gcaatgggcc gaggacggcc gcgcgcccgg
    52921 cgatccggcg ggaatcctga tgggccgttc cgggcctgtc gcgcgtgcgc gaatcctctg
    52981 cgtctgtcac gaggtcagtg aaagagacat ccttgaaacg atacaagccg gtctgccgct
    53041 cgaagagatt caacgccgtt tgaaatgcgg aacggcgtgc ggcgcctgcc tgccgacgat
    53101 caggcgaatg catggggaac acacgacaag gagtatcgaa tgacccgtat caacgatcag
    53161 accggcagcc gcgatcgacc cttcgcacgc ggcctgaacg ccggaaaggt gtggctggtg
    53221 ggagccgggc cgggcgacgc cgagttgctg acgctcaagg cgctgcgtgt tctgcaacag
    53281 agcggaatat ggttggtcga tgatctggtg ggccgcgaca tcctggcgtt cgcccgcccc
    53341 ggcacccgaa tcatcgaagt cggaaaacgc ggaggctgct cttccacacc ccaggaattc
    53401 atcctgcgcc tgatgggcag ccacgcgcgc cagggccaca cggtagcacg ggtgaagggc
    53461 ggcgatccgt tcatcttcgg acgcgcggga gaggaaatcg cctggctcac tgagcggggc
    53521 atcgaggtcg aggcggtggg cggcctcacc gcgggcctcg cggccgcgtc ggcactgggc
    53581 ctgccgctga cgcaccgcac tgtggcgcgt ggcgttgcct tggtcaccgc cagcaccgcg
    53641 gaccacacgc gccccgactg gaaagcactg gcgcgcagtg gcctgagcat cgtgtgttac
    53701 atgggcatgg cccgccccca agccttggtg gcggaatggc tggcggccgg tttcgccgcc
    53761 acgctacccg ccgccatcgt gcagcatgtt tcccgccccg atcagcggca ggcattcact
    53821 acgctggaaa atctggcctg gacaatcgag gcgcgaaaat tggccagccc cgccatcatc
    53881 gtgctgggtg aggtggtccg atacgcccag gtgaacaaca agcccgagtc actcatcgcg
    53941 ggtcggctca aacagacatc atcgatgggc acttgcggat agtcaggctt cgggggcgtc
    54001 atcc
//
LOCUS       DASTNK010000150        15728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1616318, whole genome shotgun sequence.
ACCESSION   DASTNK010000150 DASTNK010000000
VERSION     DASTNK010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15728
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616318"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /gene="yidC"
                     /locus_tag="VFR20_11560"
     CDS             <1..388
                     /gene="yidC"
                     /locus_tag="VFR20_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU0231202.1"
                     /translation="KINPLGGCLPMVIQVPVFISLYYVLLASVEMRGAPWILWIHDLS
                     APDPYYILPAIMMLTMFLQIKLNPTPPDPTQAKLMMFMPLVFGGMMFLFPAGLVLYYC
                     FNNTVSIIQQRYIMHKLDKEKAAHAR"
     gene            complement(531..1193)
                     /locus_tag="VFR20_11565"
     CDS             complement(531..1193)
                     /locus_tag="VFR20_11565"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HEU0231203.1"
                     /translation="METEFWLQRWQEGHIGFHQTRVMPLLQKYWPRAALPKGSRVLVP
                     LAGKSLDMLWLAEQGHRVLGVELSELAVGQFFAENKLRPDTHDSAMGRHYVAGDIEII
                     HGDVLTLDDATLAGCAGFYDRAALIALPPEMRKRYAERVYARLSAGCRGLLFTLEYPQ
                     EEMNGPPFSVPEVEVKALHGEAWDIDVLDRRDVLAKEPHFAERGVTRMETVAYLMERK
                     AE"
     gene            complement(1383..2258)
                     /locus_tag="VFR20_11570"
     CDS             complement(1383..2258)
                     /locus_tag="VFR20_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU0231204.1"
                     /translation="MKDKSKDEPQGMEYLQSLPRRWVTIYIPLGIFVFILLFPFYWMV
                     ITAFKPASELALHTSNPFWVIAPTLQHFKDLIFNTPFPSWMVNTLIVSIAATFVSLAA
                     GVFGAYAIERLRFQGSKQVGLAIFFAYLVPPSILFIPLASIVFQLGLFDTRWALILTY
                     PTFLIPFCTWLLMGYFRSIPYELEECALIDGATRWQILVKIILPLSVPGLISAGIFAF
                     TLSWNEFIYALTFISSSEMKTVPVGMVTELIEGDVYHWGELMAGALLGSLPVAVIYSF
                     FVEYYVSGMTGAIKE"
     gene            complement(2255..3154)
                     /locus_tag="VFR20_11575"
     CDS             complement(2255..3154)
                     /locus_tag="VFR20_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0231205.1"
                     /translation="MLSRVLNNNNVLGWLFMVPSALLLLFFLTYPLGLGVWLGFTDAT
                     IGSNGHWIGLGNYAYLVGDPLSQLALFNTVLYTVVACACKFALGLWLAILLNRKLRLQ
                     AFFRSVILLPWIVPTALSALAFWWLYDPQFSIISWALVKMGLIHHYINFLGDPWMARL
                     SIVMANIWRGIPFFAITLLAGLQTISPALYEAASIDGVTPWQQFRYVTLPLLTPIIAV
                     VTTFSVLFTFTDFQLVYVLTRGGPVNATQLMTTLSYQRAIPGGALGEGAALAILMIPF
                     LLGAILFSYFGLQRRAWQRGGDK"
     gene            complement(3264..4580)
                     /locus_tag="VFR20_11580"
     CDS             complement(3264..4580)
                     /locus_tag="VFR20_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0231206.1"
                     /translation="MAKFTRRDFLVSTAAAAATSTLAGPALAADAGTKLNYTPEKGAK
                     VHVLRWRRFVQGDEDLWNANTKKFTQQTGIPVQVDHVGWEDVRAKAALAARVGSGPDI
                     ILGWFDDPEQFPDKLMDVTDLADYLGKKYGGWYDVCKMYGMRKGKWIAIPLGVVGNAL
                     VSRDSLVKAAGFEKVPEDLPGFLKLCQALKAKGTPSGFALGHAVGDANNFAHWLLWSH
                     GGMMVNPKGQVVIQSPETIAALEYATELYKTFIPGTLGWNDSSNNKAFLDGQLSLTAN
                     GISVYYAAKNSKDPKLQAIANDIVHAHLPVGTTGKHTELMQITQMMLFKYSKFPNATK
                     AYTQFMMEAAQYNPWMEAAIGYVSQPLKAYAANPVWNNPKAKVFADSAAIMLDNGYDG
                     PLGPASAAVMADYVVVDMFAGAVSGSKTPKQAAAEAAERAKRYYKA"
     gene            complement(4653..5735)
                     /gene="ugpC"
                     /locus_tag="VFR20_11585"
     CDS             complement(4653..5735)
                     /gene="ugpC"
                     /locus_tag="VFR20_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU0231207.1"
                     /translation="MASVQIRGVQKSFGGTQVIRGVDINIDDGQFAVLVGPSGCGKST
                     LLRMLAGLEQISDGQILIADQVVNDMPPKERDIAMVFQNYALYPHMTVYDNMAFSLIL
                     AKTDRIEVKQRVDRASEILGLAQLLQRYPRQLSGGQRQRVAMGRAIVRNPQVFLFDEP
                     LSNLDAKLRVQMRAEIKALHQRLRTTSVYVTHDQIEAMTMGDLIVVMNDGRVEQCGRP
                     LDLYDRPDNLFVAGFIGSPAMNFVPGVLRRVAGGDHVELSGGARLPLPHAASGAGHDG
                     QRVVYGMRPEHLSLAPDGQGLKTRVTVIEPTGANTEVHTEIDSTELIAVFRERHDLRP
                     GDTMHLLPDVGHAHLFDAESGKVLRQ"
     gene            complement(5763..6728)
                     /locus_tag="VFR20_11590"
     CDS             complement(5763..6728)
                     /locus_tag="VFR20_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEU0231208.1"
                     /translation="MKVLITDYDFPDLDLEKAFYADAGVELVTAQCRTEDDVIRESEG
                     CQGLLVQYAPVNAKVFAARPGIRVVSRYGAGFDTINLADARDHGVWVGNSPDYGVGEV
                     ATHALAMTLALLRHVVFYDRDIKAGVWHFTSAGPMRRVSDMTLGILGLGRIGKRMAHI
                     GRNSFARVVACDPYIIDGDFPAYVERVSMEELFSLADAISLHVPLNDETRGIVNAKWL
                     GLMKPGSVLVNTARGPVVDADDLLASLDAGRLDGAALDVLPVEPPEFGARIVQHPRVL
                     LSPHAAFYSVVAEKELRRKAAQNLVDWARKGRPSYVVVEGAATGS"
     gene            complement(6749..8497)
                     /locus_tag="VFR20_11595"
     CDS             complement(6749..8497)
                     /locus_tag="VFR20_11595"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEU0231209.1"
                     /translation="MSNGNDGNESGKKEPVRRSQAWFGRQDRDGFIYRSWMKNRGIPH
                     DQFDGRPVIGICNTFSELTPCNSHFRTLAEQVKIGVWESGGFPLEFPVMSLGETMLRP
                     TAMLFRNLASMDVEESIRGNPIDGVVLLMGCDKTTPSLLMGASSCDLPTIGVSGGPML
                     SGRFRGRELGSGTDVWKMSEEVRGGRMSQEEFFEAESCMHRSHGHCMTMGTASTMASM
                     VESLGMSLPGNAAIPAVDARRNLLARASGRRIVDMVKEDLVMSKILTRQAFENAIRVN
                     AAIGGSTNAVIHLLAIAGRIGVKLDLSDWDRLGHDLPCLVDLQPSGRHLMEDFYYAGG
                     LPAVIRELGNVIHRDTLTANGHTLWDNCKDAPNWNREVIHAFSEPFKDHAGIAVLRGN
                     LCPDGAVIKPSAATPALLRHKGRAVVFEDSNDMHTRINDEDLDVDENCILVLKNCGPK
                     GYPGMAEAGNMPLPPKVLRKGITDMVRVSDARMSGTAYGTVVLHVAPEAAAGGPLALV
                     HNGDIIELDVAQRKLHLHVPEDELARRRAAWAPPAAPPRGWVKLYVEHVQQANLGADL
                     DFLAGKSGAAVPKDNH"
     gene            8989..9747
                     /locus_tag="VFR20_11600"
     CDS             8989..9747
                     /locus_tag="VFR20_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0231210.1"
                     /translation="MATGNSYDFSGRHAVITGGAAGIGLAIAQRLTHGGAHVSLWDRD
                     AAMLDRARAALPSGKAHTVAVDISNEEAVTTAAAQSQRDGGTVDILVTSAGITGPNKS
                     VCDYSVADWDQVMNINLRGPFLCCRALVPQMRPGNYGRIVNIASIAGKEGNPNASAYS
                     ASKAGVIALTKSLGKELAQTGITVNCVTPAAVHTAMFSQMTQQHIDYMLSKIPMGRFG
                     KTDEVAALVAWLCSADCSFTTGGVFDISGGRATY"
     gene            9793..10866
                     /locus_tag="VFR20_11605"
     CDS             9793..10866
                     /locus_tag="VFR20_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEU0231211.1"
                     /translation="MTPDRISTDMLRRFAGDLLKAAGLDAHIAQVVADVLVEGDLLGH
                     DTHGLALLAPYVKEIRNQAMATSGVPTVLSDHGASLCWDGRRLPGPWLVREGMDALEP
                     RARKFGSATLVIRRSHHIACLAAYLLRATERGLVMMLASSDPAVASVAPHGGTRGVFT
                     PNPIAAGIPTSGAPFLIDISSSIVTNGLSARLHKAGRRFSHPCMLDADGNPSTDPAVL
                     AQDPPGTILPLGGLESGHKGFGLALLIEALTGGLAGHGRADPPEGWGATVFMSLYDPG
                     AFGGLDAFTRQTDWLGDACRQNPPRPGAQAVRMPGDRGMARKREQERDGVALHPAIIP
                     ALVECAREYGLTFPPSSETPAAP"
     gene            10957..11700
                     /locus_tag="VFR20_11610"
     CDS             10957..11700
                     /locus_tag="VFR20_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEU0231212.1"
                     /translation="MPIKAIETQRLYRQISDQLRSLIVAGEFPAGSRLPAERDLAVQL
                     GVSRPSLREALIALEVQGYIEVHMGSGIYVCKPPPTRGKTPDLSREEGPLELIRARAL
                     LEGEVAATAARVGRKPQFDAIGDAIKAMVAVAGTGATPIEADKLFHVRIAEATGNSVL
                     VDIVARLFDFRLGPLFDQLHSHFETPEVWSDAIEEHKKILKALRSKNPDLARAAMQEH
                     MEISYKRLSSSLTRGKRRKPEAPRRKRVA"
     gene            complement(11750..14752)
                     /locus_tag="VFR20_11615"
     CDS             complement(11750..14752)
                     /locus_tag="VFR20_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEU0231213.1"
                     /translation="MSRPARFFPPSPRVSDSPLAARLRRELRGDVLFDAASRGRYATD
                     ASIYQIPPIGVVVPRDQADALLALDIARDEKAPILARGAGTSQCGQTVGEALVIDNSK
                     WLNNVVSIDPAARTATVEPGIVLDHLNARLKSHGLWFPVDVSTSAQCTLGGMAGNNSC
                     GSRSIEYGNMVHNVLAIDAVLANGTQARFGPLHGDPDGARMRSLVDGVRRIAERERGE
                     LAERMPRVLRRVAGYNLDLFDCLNPRGYTDDGVANLSHLLVGSEGTLAYTRQLTLKLA
                     PLPAHKVLGVVSFPTFYQAMDAAQHIVALKPVAVELVDRTMINLALDNPSFRPIVERA
                     LVGRPEAILLVEFAGEALAALSPRLSELDDLMGSLGLPGSVVRMTDAAAQKALWGVRK
                     AGLNIMMSMKGDGKPVSFIEDCAVPLQHLAEYTRRLTEVFHKHGTEGTWYAHASVGTL
                     HVRPILDMRRDGAAKMRAIAEEASELVREYKGAYSGEHGDGLCRGEWIAWQYGPRINQ
                     AFAEIKALFDPENRFNPDKIVNPPKMDDRRNFRFAPGYAVPRIRTALDWSVWNVRRDP
                     RSGEQSAPGTGEDKTNGFASAVEMCNNNGHCRKFDAGTMCPSYRVTRDEQHVTRGRAN
                     TLRLAISGQLGDDGLASREVKEALDLCVSCKGCKRDCPTGVDMAKFKIEARRAWAERH
                     GVTLRDRLIAFMPRYAPLASRWPRLAALADAVPVVSPWIKRKLGFAPQRSLPRFQPAF
                     LAQAAGMPPPAVRGDGRDVLLFVDTFNNCIEPENARAARRVLEAAGYTVHLNVARGER
                     SLCCGRTFLAAGLVAQAKREARRTLDALEPFVSRGVPVVGLEPSCLLSLRDEFLDYGY
                     GDEARRLAANAFLFEEFLVRERQAGRFSPPLKALPVARALVHGHCHQKAFNAFTPVQT
                     VLGWIPGLEVEVVESSCCGMAGSFGYEAEHFAASMAMAELSLLPAVRERSANAVVVAD
                     GTSCRHQISDGAATQAMHVARILADALA"
     gene            complement(14926..>15728)
                     /locus_tag="VFR20_11620"
     CDS             complement(14926..>15728)
                     /locus_tag="VFR20_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231214.1"
                     /translation="GCWKQLTEGEMNTMGDATQGLAQRLARARARHDAVGAPPQAIVP
                     NDAEAAYAVQHEILALSGVSAVGWKVGAKSADGPIQGAPLPNDGVRRSPAVFRRGDFG
                     VLGLELEIAFYFSHEFAPRERGYTDVEVWTGVRSVAATIEIVSSRFADWPKVDKFAQL
                     ADLQNHGALIVGEAVAYREDYPFLRPTLSFIYGGKDIVKGLPANPAGDPRRLLPWLVN
                     HWTARGLTVSPGMVATGGSYTGIFFPDGPGEAVGRIAGLPEVRLTISE"
BASE COUNT         2916 a         5326 c         4762 g         2724 t
ORIGIN      
        1 gaagatcaac ccgctggggg gctgtctgcc catggtcatc caggtgccgg tgttcatctc
       61 gctgtactac gtattgctgg ccagcgtgga aatgcgcggc gcgccctgga ttctctggat
      121 ccacgatctg tcggcgcccg acccttatta cattctgccc gccatcatga tgctcacgat
      181 gttcttgcag atcaagctca accccacgcc ccccgatccc acgcaggcca agctcatgat
      241 gttcatgccg ctggtgttcg gcgggatgat gttcctgttc ccggccggcc tggtgctgta
      301 ctactgcttc aacaacaccg tgtccatcat ccagcagcgc tacatcatgc acaagctgga
      361 caaggaaaag gccgcgcacg cgcggtaggc ccgcacacgg aaaaaccgtc agttcgctct
      421 gagctgacgg ttttttttcg cccgggcgcc acgtgccgcc ggttcaagcg cccgcgcttc
      481 gacagccatt cccggcgcgg cgcgccgtaa cgggaatggc catagtggcg ttattccgcc
      541 ttgcgctcca tcaggtaagc caccgtttcc atgcgggtga ccccacgctc ggcgaaatgc
      601 ggctccttgg ccaggacgtc gcggcgatcg agcacatcga tgtcccacgc ctcgccatgc
      661 aaggctttga cctccacctc aggcaccgag aaaggcggcc cgttcatctc ttcctgcggg
      721 tattccagcg tgaacaacaa tccacgacaa cccgccgaca ggcgcgcgta cacacgttcg
      781 gcgtaacgct tgcgcatctc cggaggcagc gcgatcaatg ccgcgcggtc gtagaagcct
      841 gcgcaacccg cgagggtggc gtcgtccagc gtcaggacat caccgtggat gatctcgata
      901 tcaccggcca catagtggcg ccccatggcg gaatcgtggg tgtcggggcg cagcttgttt
      961 tcggcgaaga actgcccgac cgcgagctcc gacagttcca cccccagcac gcgatggccc
     1021 tgctccgcga gccacagcat gtcgagcgac ttgccggcca gcggcaccag gacgcggctg
     1081 cccttgggca gcgccgcccg gggccagtat ttctgcagca agggcatgac acgggtctga
     1141 tgaaacccga tgtggccctc ttgccaacgc tgcagccaga attctgtttc catgtatcca
     1201 ccttcacacc gaatgagtca tgggtggatg gtactacgct cggcagacac atcgcatcaa
     1261 acgcaaagcc gccacccggt ggcggcggag aacggttctt gcgccgttga ggatgaatct
     1321 tgtgcgattg agggtggttc tcgtgccgtg ggtgaagcgc cgggtttccg cttgccggcg
     1381 gcctattcct tgattgcgcc agtcatgccc gacacgtaat actcgacgaa gaacgagtag
     1441 atgaccgcca ccggcaacga ccccagcagc gcgcccgcca tgagttcgcc ccagtgatag
     1501 acatcgccct cgatcagttc ggtgaccatg cccacgggaa cggtcttcat ttctgaggac
     1561 gaaatgaacg tcaaggcgta gatgaactcg ttccacgaca gggtgaaggc gaagatgccc
     1621 gccgaaatca gccccggcac cgacaacggc aggatgatct tcaccaggat ctgccagcgc
     1681 gtggcgccgt cgatgagcgc gcactcttcc agttcgtagg gaatggagcg gaaatacccc
     1741 atcagcaacc aggtgcagaa cggaatgagg aatgtcgggt aagtaaggat gagcgcccag
     1801 cgggtgtcga acaaccccaa ctggaacacg atggacgcca acgggatgaa caggatggag
     1861 ggcggcacca ggtaggcgaa gaagatggcc agacccacct gtttggagcc ctggaaacgc
     1921 agccgctcta tcgcataggc gccgaacacg ccggccgcca gcgacacaaa ggtggcggcg
     1981 atcgacacga tgagcgtgtt gaccatccag ctgggaaacg gcgtgttgaa gatcaggtcc
     2041 ttgaaatgct gcagcgtggg cgcgatgacc cagaacgggt tgctggtgtg cagcgccaat
     2101 tccgaggctg gcttgaaagc cgtgatcacc atccagtaga acgggaacag caggatgaac
     2161 acgaaaatgc ccagcggaat gtagatggtc acccagcgcc gcggcagcga ttgcaggtac
     2221 tccatgccct gaggctcgtc cttggacttg tccttcattt gtcacccccc cgttgccaag
     2281 cgcggcgctg caggccgaag tacgaaaaca ggatggcccc cagcaggaac gggatcatca
     2341 ggatggccag cgcggcgccc tcgcccagcg ccccgcccgg aatggcgcgc tggtacgaca
     2401 gcgtcgtcat gagttgcgtg gcgttcaccg gaccgccgcg cgtcagcacg tacaccagct
     2461 ggaagtcggt gaacgtgaac aacaccgaga acgtcgtgac caccgcgatg atgggcgtga
     2521 gcagtggcag cgtcacgtag cggaactgct gccatggcgt gaccccgtcg atggaggccg
     2581 cctcgtacaa ggcgggcgag atcgtctgca accccgcgag cagcgtgatg gcgaagaacg
     2641 ggatgccgcg ccagatgttg gccatgacga tggacaaccg cgccatccac gggtcgccca
     2701 ggaaattgat gtagtggtga atgagcccca tcttgaccag cgcccagctg atgatggaga
     2761 actggggatc gtacaaccac cagaacgcca gcgccgacaa cgccgtgggc acgatccagg
     2821 gcagcaatat caccgaacgg aagaatgcct gcagacgcag cttgcgattc agcagaatgg
     2881 ccagccacaa acccagcgcg aacttgcagg cgcaagcgac aaccgtgtag agcacggtgt
     2941 tgaacaaggc gagctgcgag agcgggtcgc cgaccagata cgcatagttg ccaaggccga
     3001 tccagtggcc gttgctgcct atggtggcgt cggtgaaccc gagccaaacg cccaatccca
     3061 gcggatacgt caggaaaaac agcagcaaca gggccgacgg caccatgaag agccagccca
     3121 gcacattgtt gttgttcagt acgcgagata gcatggacgt cctctgaacg gttttgttcc
     3181 cgcgccgtcc cggcgacccc tccatattgg tggggtcgcc gggacgagcc gggcgacctc
     3241 gggcgagccc cccgttcatc gccctacgcc ttgtaatagc gcttggcccg ctcggccgct
     3301 tcggccgccg cctgcttggg cgtcttggag ccactgaccg cgccggcgaa catgtcgacg
     3361 accacatagt cggccatgac cgccgccgat gccggcccca gaggcccgtc gtaaccgttg
     3421 tcgagcatga tggccgccga atcggcgaac accttggcct tggggttgtt ccagaccggg
     3481 ttcgccgcat aggccttgag cggctggctg acgtagccga tggcggcttc catccagggg
     3541 ttgtactggg ccgcctccat catgaactgc gtataggcct tggtggcatt cggaaacttg
     3601 gagtacttga acagcatcat ctgcgtgatc tgcatgagct cggtgtgctt gccggtggtc
     3661 cccaccggca ggtgcgcatg cacgatgtcg ttggcgatgg cctgcagctt cggatctttt
     3721 gagttcttcg ccgcgtagta caccgaaatg ccgttggccg tgagactcag ttggccatcg
     3781 aggaaggcct tgttgttgga tgaatcgttc cagccgagcg tacccggaat gaaagtcttg
     3841 tagagttcgg tggcgtactc gagcgcggcg atggtctcgg gcgactggat gacgacctgc
     3901 cccttgggat tgaccatcat gccgccatgc gaccacaaca gccagtgcgc gaaattgttc
     3961 gcgtccccca cggcgtggcc cagcgcgaag ccggacggcg tgcccttggc cttgagcgcc
     4021 tggcacagct tgaggaaccc gggaaggtct tcaggcactt tttcgaagcc ggccgccttc
     4081 accaggctgt cgcggctgac cagggcgttg cccaccaccc ccagcggaat ggcgatccac
     4141 ttgcctttgc gcatgccgta catcttgcag acgtcgtacc agccgccgta cttcttgccc
     4201 aggtagtcgg ccagatcggt gacgtccatg agcttgtcgg gaaactgttc cgggtcgtcg
     4261 aaccagccca ggatgatgtc gggcccgctg cccactcgcg ccgccaacgc ggccttggcg
     4321 cgcacatctt cccagcccac gtggtccacc tgcaccggaa tgccggtctg ctgggtgaac
     4381 ttcttggtgt tggcgttcca gaggtcttcg tcgccctgta cgaaccgccg ccaccgcagc
     4441 acatgcacct tcgcgccttt ttcaggggtg taattgagct tggtgcccgc gtcggccgcg
     4501 agggccgggc cggccagggt cgatgtcgcc gccgcggcgg cggtggacac caggaagtcg
     4561 cgtcttgtga atttagccat gtctctctcc tttggtcagg gaaaattccc gtgcaaatca
     4621 gtcatgcatg cctgtgatcc ccgtgaaaat cgttactgcc gtaaaacctt gccggattcg
     4681 gcatcgaaca ggtgcgcgtg ccccacgtcc ggcaacaggt gcatcgtgtc accgggcctc
     4741 aggtcgtggc gctcgcgaaa caccgcgatg agttcggtgg aatcgatctc ggtatggact
     4801 tcggtattgg cgccagtggg ctctatgacc gtgacgcgcg tcttcagccc ctgcccgtcg
     4861 ggcgccagcg acagatgctc gggccgcatg ccgtaaacaa cgcgctgccc gtcgtgcccg
     4921 gcgccgctgg ccgcgtgcgg cagcggcaga cgcgccccgc cggagagctc cacgtgatcg
     4981 ccaccggcca cgcggcgcaa tacccccggc acgaaattca tggcgggtga cccgatgaag
     5041 ccggcgacga aaaggttgtc ggggcggtcg taaagatcga gcggccgccc gcattgctcc
     5101 accctgccgt cgttcatgac gacgatgaga tcgcccatgg tcatggcctc gatctggtcg
     5161 tgggtgacgt agaccgacgt ggtgcgcagg cgctgatgca gcgccttgat ttcggcccgc
     5221 atctgcacac gcagcttggc gtcgaggttg gagagtggct cgtcgaacag gaacacctgg
     5281 gggttgcgca cgatggcccg ccccatggcg acacgctggc gctgcccgcc ggacagctgg
     5341 cgcggatagc gctgcaacaa ctgcgccagc cccaggattt ccgacgcgcg gtcgacacgc
     5401 tgcttgacct cgatgcggtc cgtcttggcc agtatgagcg aaaacgccat gttgtcgtag
     5461 accgtcatgt gcgggtagag cgcgtaattc tggaacacca tggcgatgtc gcgctctttc
     5521 gggggcatgt cgttgacgac ctgatcggcg atgaggatct gcccatcgga gatttgctcc
     5581 agccccgcga gcatgcgcaa caacgtggat ttcccgcacc ccgagggccc caccagcacc
     5641 gcgaactggc catcgtcgat gttgatgtcg acgccacgta tcacctgggt tccgccgaag
     5701 cttttctgta ctccccgaat ctgcaccgat gccatgcaat attcctttgt gagtgccgcg
     5761 gttcaacttc cggttgctgc gccttccacc accacataag acgggcgccc cttacgggcc
     5821 cagtcgacca gattctgcgc cgccttacgg cgcaattcct tttccgccac cacggagtag
     5881 aacgccgcgt gtggcgacaa caggaccctg ggatgctgca cgattcgagc gccgaattcc
     5941 ggaggctcga ccggcagcac gtcgagcgcc gcgccgtcca gccttcccgc atcgagcgac
     6001 gcgagcaggt catccgcgtc gaccaccggg ccgcgcgcgg tgttgaccag cacggaaccc
     6061 ggcttcatca gccccagcca cttggcattg acgataccgc gggtttcatc attgagcggc
     6121 acatgcaacg agatggcgtc ggccagggaa aacaattctt ccatagaaac gcgctcgacg
     6181 taagcaggga agtccccatc aatgatatac ggatcgcagg cgacgacccg cgcgaagctg
     6241 ttgcggccaa tgtgcgccat gcgtttgccg atgcgcccca ggcccaggat gcccagcgtc
     6301 atgtcggaca cgcgccgcat gggcccggcc gaggtgaaat gccagacccc cgccttgatg
     6361 tcgcgatcgt agaagaccac gtgccgcagc aacgccagtg tcatggccaa cgcatgcgtg
     6421 gccacctcgc ccaccccgta atcaggggag tttcccaccc acacaccatg atcgcgcgcg
     6481 tcggccaggt tgatggtgtc gaaacccgcc ccatagcggc tgacgacgcg gatgcccggg
     6541 cgcgcggcga acaccttggc attgacgggg gcgtactgca ccagcagccc ctggcagcct
     6601 tcggattcgc ggatgacatc gtcctcggtg cggcattgcg ccgtgacgag ctccacgccg
     6661 gcatcggcat aaaacgcctt ttccagatcg aggtcgggga aatcgtagtc ggtgatgaga
     6721 actttcatgt ggcgccgccg tcggcttgtc agtgattgtc tttgggtaca gcggccccgc
     6781 tcttgcccgc caggaaatcc agatccgccc ccaggttggc ctgctgcacg tgttccacat
     6841 acagcttgac ccagccgcga ggcggcgccg ccggcggcgc ccacgcggcc cgacggcgcg
     6901 cgagttcgtc ttccggcacg tgcagatgca gcttgcgctg cgcaacgtcc agctcgatga
     6961 tgtcgccatt gtgaaccagc gccagcgggc cgcccgccgc cgcctctggt gcgacgtgca
     7021 gcaccacggt gccgtaggcg gtgccgctca tgcgtgcatc ggacacgcgc accatgtcgg
     7081 tgatgccctt gcgcaggacc ttgggtggca gcggcatgtt gccggcttcg gccatgcccg
     7141 gatacccttt cggcccgcag ttcttgagca cgagaatgca gttctcatcc acgtccaggt
     7201 cttcatcgtt gatgcgcgtg tgcatgtcgt tgctgtcttc gaaaaccacg gcgcggcctt
     7261 tgtgccgaag cagcgcgggc gtggccgccg acggtttgat gaccgcgcca tccgggcaca
     7321 ggttgccccg cagcaccgcg atacccgcgt gatccttgaa aggctcgctg aatgcgtgga
     7381 tgacttcacg gttccagttg ggtgcgtcct tgcagttgtc ccagagggtg tgcccgttgg
     7441 cggtcagggt gtcacgatgt atgacgtttc ccagctcgcg gatgaccgcc ggcagaccgc
     7501 ccgcgtaata gaagtcttcc atcagatgcc ggccagacgg ctgcaggtcg accagacagg
     7561 gcaggtcatg gcccaggcgg tcccagtctg acagatccag cttcacgccg atgcgcccgg
     7621 cgatggccag cagatggatg accgcgttgg tcgacccgcc tatggcggca ttgacgcgga
     7681 tggcgttctc gaatgcctgg cgcgtcagga tctttgacat gaccaagtct tccttgacca
     7741 tgtcgacgat gcgccggccg gacgcgcgcg ccaacaggtt gcgccgcgcg tcgacggcgg
     7801 gaatggcggc gttgccgggc aggctcatgc ccagcgactc gaccatgctg gccatggtcg
     7861 acgccgtgcc catggtcatg caatggccat gcgaccgatg catgcagctc tcggcctcga
     7921 agaattcttc ctgagacatg cgcccgccac ggacttcctc ggacattttc cagacgtcgg
     7981 tgccggaacc cagttcacgc ccccggaacc gcccgctgag catgggcccg cccgacaccc
     8041 caatggtggg cagatcgcag gacgacgcgc ccatcagtag tgaaggcgtg gtcttgtcgc
     8101 aacccatgag cagcacgacc ccatctatgg ggttcccgcg gatggactct tcgacatcca
     8161 tgctggccag attgcgaaac agcatggctg tcggccgcaa catggtttcg cccagcgaca
     8221 tcaccgggaa ttcaagagga aacccgcccg actcccagac gccgatcttg acctgttcgg
     8281 ccagggtgcg gaagtgcgag ttgcaaggag tgagctcaga gaaagtgttg cagatgccaa
     8341 tgaccggtcg accgtcgaac tggtcgtgcg gaatgccgcg attcttcatc catgaccggt
     8401 agataaagcc gtcgcgatcc tggcgcccga accacgcctg gctgcgccgc acgggttcct
     8461 tcttgccaga ttcgttgccg tcattgccgt tgctcaatcg gatctcccgg gcacggccgc
     8521 ctgtgcatac cgccgtgccc ggccgcggcg gtatgcggcc gacgcgccct gcaatggcgc
     8581 gccacgactt cgaggcaggt tctcgcacac ggctgttcgc gcgggtcgga gcctggccgg
     8641 caaccagtct attacacaaa tcggaatggg taaagtggta ttaccaaagc actgaaggcc
     8701 gcgccccggg atgtaagaag tccccgccga acaagtcctt tccccttgcc attgacaacc
     8761 ctggccggct atggaattct agctttattg gcccgaccag cttaccggtt tcaagatcca
     8821 ttcattgcat tcacttcacg ccattcgcaa gatgccaagg ctgtttgccg gcccaaaccc
     8881 gatgcgcaac ggaacactcg tcggcgccac gccagcgtgc tgtccggttg gctcgtgaat
     8941 tcaaaaatca atcaaaccac acactcgcca ccaaaaggat tcctcgtcat ggcaaccggc
     9001 aattcctacg atttttccgg caggcatgcc gtcatcactg gcggcgcggc gggtatcggc
     9061 ctggccatag cccagcgcct gacccatggc ggagcccacg tcagcctgtg ggatcgcgat
     9121 gccgccatgc tcgacagggc gcgggccgcc ctgccttccg gcaaggcgca taccgtggcc
     9181 gtcgacatca gcaatgaaga agccgtcacc accgccgccg cgcaaagcca gcgggatggc
     9241 ggcaccgttg atatcctggt caccagcgcc ggcatcaccg gccccaacaa atcggtttgc
     9301 gactactccg tggccgactg ggaccaggtc atgaacatca acctgcgcgg tccgttcctg
     9361 tgctgccgcg ccctcgtgcc gcagatgcgg cccggcaact acgggcgtat cgtcaacatc
     9421 gcctccatcg ccggcaaaga gggcaacccc aatgcctccg cctacagcgc ctccaaggcg
     9481 ggcgtcatcg cgctgacaaa atcgctgggc aaggaactcg cgcaaaccgg catcacggtc
     9541 aactgcgtca cgccggcggc ggtgcatacc gccatgttct cgcaaatgac gcagcagcac
     9601 atcgattaca tgctctcgaa aatccccatg ggccgcttcg gcaagaccga tgaggtcgcc
     9661 gcgctggtag cctggctttg ctcggccgat tgctcattca ccacgggcgg ggtgttcgac
     9721 atttccggcg gccgggccac gtattaaact ggttctcaca cctttcctct tcatactgga
     9781 aacccgcctc acatgacgcc cgaccgcatt tccaccgaca tgctgcggcg cttcgccggg
     9841 gatctcctga aagcggcggg gctggacgcc catatcgccc aagtcgtcgc cgacgttctc
     9901 gtcgagggcg acctcctggg ccacgacaca catggcctgg cgttgctggc cccttacgtc
     9961 aaggaaatcc gcaaccaggc catggccact tcgggcgtgc ccacggtgct gtccgatcat
    10021 ggggcctcac tgtgctggga cggccgccgc ctacccggcc catggctggt gcgcgaaggc
    10081 atggacgctc tcgaaccccg cgcgcggaaa ttcggcagcg ccacactggt catccgccgc
    10141 agccatcaca tcgcctgtct cgcggcctac ctgctgcgcg ccaccgagcg cggtctggtc
    10201 atgatgctgg ccagctccga ccccgcagtc gccagcgtcg caccccacgg cggcacccgg
    10261 ggcgtcttca cacccaaccc catcgccgca ggcatcccca cgtcgggtgc gccgttcctc
    10321 atagacatct cgtcatccat cgtcaccaac ggactcagcg cccggctgca caaggccggc
    10381 cggcgcttca gccacccctg catgctcgat gccgacggca acccaagcac cgaccccgcc
    10441 gtcctggcgc aagatccacc tggcaccatc ctacccctgg gcggcctgga gtccgggcac
    10501 aaggggttcg ggctggcgtt gctcatcgag gcgctcaccg gtggcttggc cggtcatggg
    10561 cgggccgacc cacccgaggg ctggggcgca acggttttca tgtcgctgta cgatccgggc
    10621 gcgttcggcg gtctggacgc tttcacgcgc cagaccgact ggctgggcga cgcctgccgc
    10681 caaaatccac cacgtcccgg cgcgcaagcc gtacgcatgc caggagaccg cggcatggcg
    10741 cgcaaacgcg aacaagaacg cgacggcgtg gcgctgcatc cggccatcat cccggcactg
    10801 gtagaatgcg cccgcgaata tgggctgaca tttcccccct catcagaaac tcccgccgcg
    10861 ccatgacggc gcggtggccc ggacttcgaa cacccgtgta ccgtgtgacc ggttcttgca
    10921 ctcgaattgg ccaaacagca caccaggacg cccctcatgc ccattaaagc cattgaaacc
    10981 cagcggctct accgccagat cagcgaccag ctccgctccc tgatcgtggc gggcgaattc
    11041 ccggccggct cgcgcctgcc ggccgaacgc gatctcgccg tgcagctcgg cgtcagccgc
    11101 ccttcgctgc gtgaagcctt gatcgcactc gaggtccagg gctacatcga agtccacatg
    11161 ggctccggca tctatgtgtg caaaccgcct cccacgcgcg gtaaaacccc cgatctatcg
    11221 cgcgaagaag ggccgctgga actcatccgc gcccgcgcgc tgctcgaggg cgaagtggcc
    11281 gccaccgccg cccgggtcgg gcgcaagcca caattcgacg ccatcggcga cgccatcaag
    11341 gccatggtcg cggtggccgg cacgggcgcc acgcccatag aggccgacaa actgttccat
    11401 gtccgcatcg ccgaggccac tggcaacagt gttctggtcg acatcgtggc gcggctgttc
    11461 gacttccgcc tgggcccgct gttcgaccag ttgcacagcc atttcgaaac tcccgaggtc
    11521 tggtccgacg ccatcgaaga gcacaagaaa atcctcaagg cgctacgctc gaagaatccc
    11581 gatctggccc gtgccgccat gcaggaacac atggaaatct cgtacaagcg gctcagctcg
    11641 agcctgacac gcggcaaacg gcgcaagccg gaggcgccgc gccgcaagcg tgtggcctga
    11701 caccactgcg ctgtttggac ggggcctgtt cacgcccccc ttctactcgt caggcgagcg
    11761 cgtcggccaa tatccgcgcc acgtgcatcg cctgtgtcgc ggccccatca ctgatctgat
    11821 ggcggcagct ggtgccatcg gccaccacca cggcgtttgc tgaccgctcc cgcacggcgg
    11881 gcagcaagga aagctccgcc atggccatcg acgccgcgaa gtgctccgcc tcgtagccga
    11941 agctgcccgc catgccacag caggatgatt ccaccacctc gacctcaagc cccggaatcc
    12001 agcccagcac cgtctgcacc ggcgtgaacg cattgaacgc cttctggtgg caatggccat
    12061 gcaccaacgc gcgcgccacc ggcagcgcct tcaatggcgg agagaagcgc ccggcctgcc
    12121 gctcccggac aagaaactct tcgaacagga aggcgttcgc cgccagacgc cgcgcctcgt
    12181 cgccgtaccc gtagtccagg aactcgtcgc gcaacgatag gagacacgac ggctccagcc
    12241 ccaccacggg aaccccccgg ctcacgaacg gctccagggc atcgagcgtg cggcgcgcct
    12301 cccgcttcgc ttgcgccacg aggccggccg ccagaaacgt gcggccacag catagtgacc
    12361 gctcgccacg cgcgacattc aaatgcaccg tgtagcccgc cgcctccagc actcgccgcg
    12421 ccgcgcgcgc attctcgggt tcaatgcagt tgttgaacgt gtcgacgaaa agcaggacat
    12481 ccctgccgtc gccacgcacc gccggtggcg gcattccggc cgcttgcgca agaaacgccg
    12541 gctggaagcg cggcaacgaa cgctgcggcg cgaaccccaa cttgcgcttg atccagggcg
    12601 acacgaccgg gacggcatcc gccagcgcgg ccaggcgggg ccaccggctg gccagcggcg
    12661 cgtaacgcgg catgaaggcg atcagacgat cacgcagcgt gacgccgtgc cgctcagccc
    12721 aggcccggcg cgcctcgatc ttgaacttcg ccatatcgac cccggtcggg caatcgcgct
    12781 tgcagccctt gcaggacacg cacagatcaa gcgcttcctt gacctctcgg ctcgccaggc
    12841 cgtcgtcacc cagttgtccg gagatcgcca gcctcagggt attcgcccgc ccccgggtga
    12901 catgttgttc gtcgcgcgtc acgcggtagc tggggcacat ggtgccggca tcgaacttgc
    12961 ggcagtggcc gttgttgttg cacatctcga cggcgctggc gaagccattc gtcttgtcct
    13021 cgcccgtgcc cggcgcgctt tgctcgccgc tccgggggtc gcgccgcacg ttccacacgg
    13081 accagtccag tgccgtgcgg atgcgcggca cggcgtagcc gggagcgaat cggaagttgc
    13141 gtcgatcatc catcttcggc gggttcacga tcttgtccgg attgaagcgg ttttccggat
    13201 cgaacaacgc cttgatctcg gcgaacgcct ggttgatacg agggccgtat tgccaagcga
    13261 tccactcccc acgacacaat ccgtcgccat gctcgcccga atacgcgccc ttgtactcgc
    13321 gcaccaactc cgacgcctct tccgcgatgg cgcgcatctt cgcggcgcca tcgcgccgca
    13381 tgtcgagaat gggacgcacg tgcaacgtgc ccacgctggc gtgcgcgtac cacgtgccct
    13441 ctgtgccgtg cttatgaaac acctctgtca gccgacgcgt atattcggcc agatgctgga
    13501 gcggtaccgc gcagtcctcg atgaacgaca ccggcttgcc atcgcccttc atgctcatca
    13561 tgatgttcag gccggccttg cgcacgcccc atagcgcctt ctgggccgcg gcgtcggtca
    13621 tgcgcacgac cgacccgggc aggccgaggc tgcccatcag atcgtccagt tccgacagac
    13681 gcggcgacaa cgccgccagc gcttcgccgg cgaactcgac cagaagaatc gcctcgggcc
    13741 gccccaccag cgcccgttca acaatggggc gaaacgacgg attatccagc gccaggttga
    13801 tcatcgtgcg atcgaccagt tccaccgcca cgggcttgag cgcaacgatg tgctgcgccg
    13861 catccatggc ctgatagaac gtcgggaagc tgaccactcc cagcactttg tgcgccggca
    13921 gaggcgccag cttgagcgtc agttgccgcg tgtaggcgag cgtgccctcc gaaccgacca
    13981 acaggtgcga cagattcgcc acgccatcat cggtatagcc acgcgggttc aagcagtcga
    14041 acagatcaag gttgtaaccg gcgacgcggc gcagcaccct gggcatgcgc tcggcgagct
    14101 caccgcgctc gcgttcggcg atgcggcgca cgccatccac gagcgaccgc atgcgcgcac
    14161 cgtccggatc accgtgcaac ggcccgaagc gcgcctgtgt gccattggcc agcaccgcat
    14221 cgatggccag cacgttgtgc accatgttcc cgtattcgat ggagcgcgac ccgcacgagt
    14281 tgttgccggc cataccgccc agcgtgcact gcgcgctggt ggaaacgtcc accggaaacc
    14341 acaggccgtg cgatttgagt cgcgcgttca gatgatcgag cacgatgccc ggctccaccg
    14401 tcgccgtgcg cgccgccggg tcgattgaaa ccacattgtt cagccattta ctgttgtcga
    14461 tgaccagggc ttcacccacc gtctgcccgc attgacttgt gcccgccccg cgcgccagaa
    14521 tcggtgcctt ctcatcgcgt gcgatgtcca gcgccagcag cgcatccgcc tgatcgcgcg
    14581 gcaccacgac gccaatgggc gggatttggt agatggaggc atcggtcgca taccggccac
    14641 gactggccgc atcgaacagg acatcgccac gcagttctct gcgcagccgc gccgccagtg
    14701 gagaatcgct gacgcgaggg gatggaggga aaaagcgagc aggacgagac atgatcggcc
    14761 catcttgaaa aattctgcga gtcatgaggc ggcctttgtc gtcgtgaaac agcttcaggg
    14821 ttggttgcct ggggaccctc tgagcaggcc accgcgtcat acgcgtctat ggcttgggcg
    14881 gatctgacga tatggaagga aatgctccct cgtcgagacg gccactcact cggagatggt
    14941 cagccgcact tccggcagac cggcgatccg ccccactgcc tcacccggcc cgtcggggaa
    15001 gaatatgccc gtgtacgacc ccccggtggc gaccattccg ggagacaccg tcaggccgcg
    15061 cgcggtccag tggttcacca accatggcaa caagcgtcgt gggtcgccgg cgggattcgc
    15121 gggcaaccct ttcacaatgt ccttgccacc gtaaataaag gacaaggtcg gccgaagaaa
    15181 gggatagtct tcgcggtacg ccacagcctc ccccacgatc aacgcgccgt ggttctgcag
    15241 atccgccaac tgggcgaact tgtccacctt cggccagtcg gcaaaacggc tcgacacgat
    15301 ctcgatggtg gccgcgaccg agcgcacgcc cgtccacacc tcgacgtccg tgtatccccg
    15361 ttcgcgcgga gcgaattcat ggctgaagta gaaggcgatc tccagctcca ggcccagcac
    15421 gccgaagtcc ccgcgccgaa acacggcagg gctgcgccgc acgccgtcgt tcggcaatgg
    15481 cgcgccctga atcggaccgt cggccgactt ggcgccgact ttccacccca ccgccgatac
    15541 gccgctcaag gccaggatct cgtgttgcac ggcgtaggcg gcctctgcat cgttcggcac
    15601 gattgcctgc ggcggcgcac ccaccgcatc atgccgcgcc ctcgcccgtg ccagacgctg
    15661 cgccagtccc tgtgtggcat cacccatggt gttcatctcc ccttccgtca attgtttcca
    15721 gcacccat
//
LOCUS       DASTNK010000151         5718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1434295, whole genome shotgun sequence.
ACCESSION   DASTNK010000151 DASTNK010000000
VERSION     DASTNK010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434295"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VFR20_11625"
     CDS             complement(<1..559)
                     /locus_tag="VFR20_11625"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0231215.1"
                     /translation="MNITVNGATVDAEKWPTPRLAAVHELLRQRARELGLLDTTANED
                     ETKQAIERTLAREVAVPVAQEEECRRWYEANATRFRSGDLVFARHILFQVTPGVPVPA
                     VRAVAEAILHEIRRDSALFDKHAREHSNCPSGATGGQLGQLQRGDTVPEFEKAIFEDA
                     TIGVLPKLVATRYGFHIIAVDKRVEG"
     gene            complement(556..1257)
                     /gene="narI"
                     /locus_tag="VFR20_11630"
     CDS             complement(556..1257)
                     /gene="narI"
                     /locus_tag="VFR20_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004528383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEU0231216.1"
                     /translation="MPAYLNEFLFGIYPYICLAVLLLGSLIRFDREPYSWKSDSSQIL
                     HAGQLKLGSNLFHYGVLVVILGHFAGFLMPEAIVMALMTPTYHQLLAMVVGGIAGVVA
                     LIGATILIHRRLSYRRIRANSRKWDIAIVIMLWLQLLLGVATVPLSAQHMDGALFEQL
                     VQYVQGVVLFHGHNAALMQNVPWVYQFHILLGFTIFLVSPFTRMVHIWSGVATVAYLF
                     RPYQLVRKRGGSGHS"
     gene            complement(1283..1924)
                     /gene="narJ"
                     /locus_tag="VFR20_11635"
     CDS             complement(1283..1924)
                     /gene="narJ"
                     /locus_tag="VFR20_11635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HEU0231217.1"
                     /translation="MTPNPVLLLRALSVLLTYPDAEFRAALPEIAEAIRGARALDKRL
                     RVDLLALVDEIANSDSLEAEGRYVDLFDRGRHTSLNLFEHVFGDGRQRGPAMLELRER
                     YLAAGMEPLGEELPDHLPLLLEYLSCRDLAETRDTIGEVAHVVRKLGNTLAHRHSRYA
                     AVMAAVLALGGERGLDARAPAPPPDDFDRSWEEKPAFDPSDLSPTVVPRHVAP"
     gene            complement(1931..3472)
                     /gene="narH"
                     /locus_tag="VFR20_11640"
     CDS             complement(1931..3472)
                     /gene="narH"
                     /locus_tag="VFR20_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011205549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEU0231218.1"
                     /translation="MKVRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRKGMEYAWFNNV
                     ETKPGIGYPKDWENQEHWKGGWERKDNGKLVPRQGGRWGVLARIFSNPNLPQIDDYYE
                     PFTFDYENLQTAPELPTAPVARPISLVSGRTMEKIEWGPNWEEILGGEFDKRSKDYNF
                     EAVQKDIYGQFENTFMMYLPRLCEHCLNPTCVASCPSGSIYKREEDGIVLIDQDKCRG
                     WRMCVSGCPYKKIYYNWVSGKAEKCTFCYPRIEAGMPTVCSETCVGRIRYLGVLLYDA
                     DRIEAAASTESPQDLYQAQLDVFLDPSDPEVQRQALADGIAESWIEAAQHSPTYKMAV
                     EWKIAFPLHPEYRTLPMVWYVPPLSPITAHANAGAIESAANGLPDVESLRIPVRYLAN
                     LLTAGNEPPIVGALQRMMAMRAWFRAKQLGEAQDPALLASVGLDEATAQSMHRYLAIA
                     NYEDRFVIPTAHREESENAYQIRGGCGFTFGNGCDGIDAKAGLFGGKMGRRNVVPIRE
                     LDDSE"
     gene            complement(3469..>5718)
                     /locus_tag="VFR20_11645"
     CDS             complement(3469..>5718)
                     /locus_tag="VFR20_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004548292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HEU0231219.1"
                     /translation="WQETITGVPAARAIEVARGFASSAEKTHGRSMVIIGSGMNHWFH
                     QDVSYRAIINTLMLCGTIGVSGGGWSHYTGQEKVRPQTGWAALAFALDWVRPPRHMAG
                     TSFFYLHSDQWRYEQLGVGGLLSPLADRSRQTGALVDCDVRAQRMGWMPSAPQLNRNP
                     LTVAREAMRSGGDPKQYIVDALKSGKLAMACEDPDNPDNFPRNLFVWRSNLLGSSGKG
                     HEYFLKHFLGTRCGLQGQDLGAEGGAKPEEVTWRDAAPEGKLDLVVTLDFRMSTTALY
                     SDVVLPTASWYEKNDLSTTDMHPFIHPFSVAVDPVFGSKSDWETFKALAKKFSELAQG
                     ELGEEKDVVLTPIMHDTVNEMAQPFDVLDWKKGECDLIPGKTGPGITFIDRDYPAVHA
                     RFTSLGPLLDTLGNGGKGIHWDMRHEVEELGALNRTVPDGPAKGRPCIVSDIDAAETI
                     LMLAPETNGEVAVRAWEALSKTTGRSHRHLADARADEKIRFRDIQAQPRKIITSPTWS
                     GIESEKVSYNAGYINVNELVPWRTLTGRQHFYLDHAWIRDFGEAFALYRPPIDTRAVN
                     ALLGKKGNGNREIVLNFITPHQKWGIHSTYTDNLIMLTLSRGGPIVWISEVDARQADI
                     QDNDWIECFNANGALTARAVVSQRVNQGMCLMYHAQEKIVNVPGSEVTGMRGGIHNSV
                     TRIIVKPTQMIGGSAQFSWSFNYYGPVGSDRDGFVILRKMAKVDWMDHIQRQAREAEI
                     NAKLAGAEK"
BASE COUNT         1053 a         1874 c         1695 g         1096 t
ORIGIN      
        1 tgccctcgac gcgcttgtcg acggcgatga tgtgaaagcc gtaacgcgtg gccacgagct
       61 tgggcaacac cccgatcgtg gcgtcttcga agatggcctt ctcgaattcc ggcaccgtat
      121 cgccgcgctg caattgcccc aactgcccac cagtggcgcc cgacgggcag ttcgagtgct
      181 cacgggcatg cttgtcgaaa agcgcagaat ccctacggat ttcatggaga atcgcctcgg
      241 cgaccgcgcg cacggccggc acgggtaccc ccggagtgac ctgaaacagg atgtgccgcg
      301 caaagaccag gtcgccactg cggaagcgcg ttgcattggc ctcgtaccag cgccggcact
      361 cttcctcttg ggccacgggc accgccactt cccgggccag cgtgcgctcg atcgcctgtt
      421 tggtctcgtc ctcgttggcc gttgtgtcga gcagccccaa ttcacgcgcg cgctgacgca
      481 gcaattcgtg caccgccgcc agacgcggcg tgggccattt ctcggcgtcg accgttgcgc
      541 cgttgacggt gatgttcatg agtgccccga ccccccgcgt ttgcgaacca gttgatacgg
      601 ccggaacaga taggcaaccg tggccacgcc gctccagata tgcaccatgc gtgtgaaggg
      661 cgacaccagg aagatggtga agccaagcag aatgtggaac tggtataccc acggcacgtt
      721 ttgcatgagg gccgcgttat gcccatgaaa cagcacgacc ccctgcacgt attgcacgag
      781 ttgctcgaac agcgccccat ccatgtgctg tgccgacagt ggcaccgtgg ccacacccag
      841 cagcaattgc agccacaaca tgatcacgat cgcgatgtcc catttgcggc tgttcgcccg
      901 tatgcggcga taggacaggc gccggtggat caggatcgtg gcgccgatca gggcgaccac
      961 cccggcgatg ccgcccacca ccatcgccag caactggtga taggtggggg tcatcagcgc
     1021 catcacgatc gcctcgggca tcaggaagcc ggcgaaatgc cccagtatga ccaccagcac
     1081 cccgtagtga aacaggtttg atcccagctt cagttgcccc gcgtgcagga tctgggatga
     1141 atcgctcttc catgagtacg gctcgcggtc gaagcgtatc aggctgccca gaagcagcac
     1201 cgccagacaa atgtagggat aaatgccgaa caggaactca ttgagataag cgggcaaagt
     1261 catggctgga cctccgggtg gatcatggcg cgacgtgccg aggcacgacc gtcggagaca
     1321 ggtcgctggg atcgaacgcg ggcttttcct cccacgaacg gtcgaaatcg tcgggaggcg
     1381 gcgccggagc cctggcatcc aggccgcgtt caccgcccag cgccaacacg gcggccatca
     1441 ccgccgcgta acggctgtgg cgatgggcca atgtattgcc aagcttgcgc acgacatgcg
     1501 cgacctcgcc aatggtgtcg cgcgtttcgg ccaggtcgcg gcagctcagg tattcaagca
     1561 acaacggcag gtgatcaggc agttcttcac ccaagggttc catgcccgcg gccaggtacc
     1621 gctcgcgcaa ctccagcatg gccgggccgc gctggcgccc atccccgaat acatgttcga
     1681 acagattcaa cgacgtgtgc cgcccgcgat cgaacaaatc cacgtagcgc ccctcggcct
     1741 ccagggaatc gctgttggcg atctcatcga ccagcgccag cagatcgacg cgcagccgtt
     1801 tgtcgagcgc gcgcgccccc cggatcgctt cggcgatctc gggtagcgca gcgcggaatt
     1861 ctgcgtcagg gtatgtcagc aggaccgaca acgcccgcag cagcagaaca ggattaggcg
     1921 tcatgactcg tcactcggaa tcgtcgagct cacggatcgg caccacattg cgccggccca
     1981 tttttccccc gaacagcccc gccttcgcat cgatcccgtc acagccattg ccgaacgtga
     2041 acccgcaccc accccggatc tggtaggcgt tttccgactc ctcgcgatga gcggtcggaa
     2101 tcacaaagcg gtcttcatag ttcgcgatcg ccaagtaccg gtgcatggat tgcgccgtgg
     2161 cctcgtccaa ccccacgctg gccaacaacg ccgggtcctg cgcctcgccc agttgcttcg
     2221 cgcggaacca ggcacgcatc gccatcatgc gctgcagggc gccgacgatc ggcggttcgt
     2281 tgccagccgt cagcaggttg gcgaggtaac gcacgggaat gcgcagcgat tcaacgtcgg
     2341 gtagtccatt ggcggcgctt tcgatggccc ccgcattcgc gtgggccgtg atgggagaca
     2401 acggcggcac gtaccacacc atcggcagcg tccggtactc ggggtgcagc ggaaaggcga
     2461 ttttccattc caccgccatc ttgtacgtgg gcgaatgctg ggccgcctcg atccaggatt
     2521 cggcgatgcc atccgccagc gcctggcgtt gtacctcggg gtcggagggg tcgagaaaga
     2581 catcgagttg cgcctgatac aggtcttgcg ggctctcggt gcttgccgcg gcctcgatcc
     2641 ggtcggcatc gtacagcagc acccccagat agcggatgcg gccgacacag gtttcggaac
     2701 acaccgtcgg catgcccgct tcgatgcgcg gataacagaa ggtgcatttc tcggccttgc
     2761 cgctcaccca gttgtaatag attttcttgt acggacaacc cgatacgcac atccgccagc
     2821 cccggcactt gtcctggtcg atcaggacga tgccgtcttc ctcgcgtttg tagatggatc
     2881 ctgacgggca cgacgccaca catgtgggat tcaggcagtg ctcgcacaac cggggcagat
     2941 acatcatgaa cgtgttctcg aactgcccat agatgtcctt ctgcaccgcc tcgaagttgt
     3001 agtccttgct gcgcttgtcg aactcaccgc ccaggatctc ctcccagttc ggcccccatt
     3061 cgattttctc catcgtgcgc cccgacacca gggaaatggg gcgtgccacc ggcgccgtcg
     3121 gcagttccgg cgcggtctgc agattctcgt agtcgaacgt gaagggttcg tagtagtcgt
     3181 cgatctgcgg cagattcggg ttggagaaaa tgcgcgccag cacgccccag cgcccgccct
     3241 gcctggggac cagcttgccg ttgtccttgc gctcccagcc ccctttccag tgctcctggt
     3301 tttcccaatc cttggggtac cctatgcccg gcttggtttc cacgttattg aaccaggcat
     3361 actccatgcc cttgcgcgac gtccacacgt tcttgcacgt caccgaacag gtatggcagc
     3421 cgatgcactt gtccagattc aacaccatcg ccacttgcgc gcgtactttc atttttcggc
     3481 tcccgccagc ttcgcattga tctcggcctc acgcgcctgg cgctgaatgt ggtccatcca
     3541 gtcgaccttg gccattttcc tgaggatgac gaacccgtcc cgatcggaac ccaccgggcc
     3601 gtaatagttg aagctccatg agaactgcgc cgaaccgccg atcatctgcg tgggtttgac
     3661 gatgatgcgt gtgacggagt tgtggattcc gccacgcatg cccgtgactt ctgaccccgg
     3721 cacattcacg attttctctt gtgcgtgata catcaggcac atgccctggt tcacacgctg
     3781 gctcaccacc gcgcgcgcgg tgagcgcgcc attcgcgttg aagcactcga tccagtcgtt
     3841 gtcctggatg tcggcttgcc tggcgtccac ctcggatatc cagacgatcg gcccgccccg
     3901 cgacagcgtg agcataatca ggttgtcggt gtaggtgctg tggatgcccc atttctgatg
     3961 cggcgtgatg aaattcagca cgatctcacg attgccgttg cccttcttgc caagcaacgc
     4021 attcaccgcg cgggtatcga tgggcgggcg atacagcgcg aaggcctcgc cgaaatcgcg
     4081 tatccaggca tgatcaaggt agaaatgctg ccgccccgtc aaggtacgcc acggcaccag
     4141 ttcgttgacg ttgatgtaac ccgcgttgta gctcactttc tcgctttcga tgccggacca
     4201 cgtgggcgaa gtgatgatct tgcgtggttg ggcctggatg tcccggaagc ggatcttctc
     4261 gtcggcccgg gcgtcggcca agtgacggtg actgcgtccc gtggtcttgg acagcgcctc
     4321 ccaggcgcgc accgccacct cgccgttggt ttcgggtgcc agcatgagga tcgtctcggc
     4381 cgcatcgatg tcactgacaa tgcacggccg ccccttggcg ggaccatcgg gcaccgtgcg
     4441 gttgagcgcg ccgagttcct cgacctcgtg ccgcatgtcc caatggatac ccttgccgcc
     4501 gttgccgagc gtgtccaaca gcggcccgag cgacgtgaaa cgggcatgca cggcgggata
     4561 atcccgatcg atgaaggtga tgccgggacc ggtcttcccg ggtatcaggt cgcattcgcc
     4621 ctttttccag tcaagcacat cgaacggctg cgccatctcg ttgacggtgt cgtgcatgat
     4681 gggcgtgagc accacatctt tttcctcgcc cagctcgccc tgggcaagtt ccgaaaactt
     4741 cttggcgagc gccttgaagg tttcccaatc gcttttcgac ccgaacaccg ggtccaccgc
     4801 caccgaaaac gggtggatga acggatgcat gtcggtcgtg gagagatcgt tcttttcata
     4861 ccacgaagcc gtcggcaaga ccacatcgga atacaacgcc gtcgtcgaca tgcggaagtc
     4921 cagggtcacc accaggtcga gcttgccctc gggagcggcg tcgcgccacg tgacttcctc
     4981 gggtttcgcc ccaccctctg cgcccagatc ctggccctgc aggccgcatc gcgtacccag
     5041 gaaatgcttg aggaaatact cgtgcccctt gcccgacgac cccagcagat tggagcgcca
     5101 cacgaacagg ttgcgtggaa agttgtccgg gttgtcggga tcttcgcagg ccatggcaag
     5161 cttgccagac ttgagcgcat ccacgatata ttgtttcggg tcaccccctg accgcatggc
     5221 ctcgcgcgcc accgtgagcg gattgcgatt caactgggga gccgagggca tccagcccat
     5281 gcgctgcgca cgaacatcgc aatcgacaag cgccccggtc tgtcgtgagc ggtcggcaag
     5341 cggggacagc aggccgccga caccgagttg ctcatagcgc cattggtcgg aatgcaggta
     5401 aaagaacgac gtgcccgcca tgtggcgcgg cgggcgaacc cagtccagcg cgaacgccag
     5461 cgctgcccat ccggtttgcg ggcgcacttt ttcctggccc gtgtagtgcg accagccgcc
     5521 gcccgacacg ccaatcgtgc cacacagcat cagtgtgttg atgatggccc ggtagctgac
     5581 atcctggtgg aaccagtggt tcatgcccga gccaatgatc accatcgagc ggccatgtgt
     5641 cttctcggcg ctcgaggcga acccccgcgc cacctcgatc gccctggcag cgggcacccc
     5701 ggtgatcgtc tcttgcca
//
LOCUS       DASTNK010000152         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_694207, whole genome shotgun sequence.
ACCESSION   DASTNK010000152 DASTNK010000000
VERSION     DASTNK010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694207"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFR20_11650"
     CDS             <1..240
                     /locus_tag="VFR20_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0231220.1"
                     /translation="GITVNVVAPGPIQTDMFYDVIPAGSEREHAIAQGIPVKRLGRAD
                     DVARAVMFFSDPANSFVTGQTLFVCGGASVSSITI"
     gene            456..1604
                     /locus_tag="VFR20_11655"
     CDS             456..1604
                     /locus_tag="VFR20_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0231221.1"
                     /translation="MKLKTLATTVAALTLLGTTAAFAQVKIGVVSSTTGPTALIGIPQ
                     KNTVALLPKKVGDLTIQYISLDDGSDPTKTVVDFKKLISEDKVDAIIGPSGSPNAMGV
                     IQFAAQSGTPMLAPVGTASVVLPMTPEKKWVFKTTQNTDIIAKALIGHMVKAGVKTLG
                     FIGLNDAYGTDWLNVISKMAKAHNIKIVAVERYQRSDSSVTGQALRVLAAKPDAVLVA
                     GTGAAAVLPETTLVKQGYKGQFYQTHGAALPAFLKLGGKAVNGTILAASLMLVLPEIS
                     DSNPSKKIAEQYDASYEKLYGHKPATFGANVYDAGLLLEKAIPKAEKKAKPGTKEFRS
                     ALRDALEQTHELVATQGVYNMTPEDHSGFDDRGRELIIVKNQQWHLLK"
     gene            complement(1712..2572)
                     /gene="fghA"
                     /locus_tag="VFR20_11660"
     CDS             complement(1712..2572)
                     /gene="fghA"
                     /locus_tag="VFR20_11660"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEU0231222.1"
                     /translation="MPGGLELTSEHRCFDGVQRFYRHDSQAIGLPMRFSVFLPPQAAA
                     GRVPVLFFLAGLTATEENFMVKAGAQRLAAKHGLMLVSPDTSPRGAGVPGDEDDWDFG
                     TGAGFYLDATQAPWKTHYRMESYVARELFDIATGELPGDAGRVGIFGHSMGGHGALVL
                     AQRHRDQYRSVSAFAPIAAPSRCPWGQKAFTGYLGDDHASWAEHDASLLMARSNCPYP
                     GGILVDQGLADAFLDEQLYPGEFEAACAKAGQPLTLRRHEGYDHGYYFLSTFMEDHLA
                     FHAERLKAVS"
     gene            complement(2572..>3096)
                     /locus_tag="VFR20_11665"
     CDS             complement(2572..>3096)
                     /locus_tag="VFR20_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU0231223.1"
                     /translation="LGGIGLNVIQGAKMVGAGKIIGVDLNPGREALARKFGMTHFVNP
                     KDVDNVVDHIVSLTDGGADYSFECIGNVKVMRQALECCHKGWGQSIIIGVAEAGAEIS
                     TRPFQLVTGRHWKGSAFGGARGRTDVPKIVDWYMEGRLNIDDLITHTLPLEKINDGFD
                     LMHRGESIRSVVVY"
BASE COUNT          659 a         1023 c          897 g          517 t
ORIGIN      
        1 ggcatcaccg tcaacgtggt ggcgccgggc cccatccaga ccgacatgtt ctatgacgtg
       61 atcccggccg ggagtgaacg cgagcacgcc atcgcacagg gcattccggt caaacggctg
      121 ggtcgcgccg acgacgtcgc ccgcgcggtg atgtttttct ccgatccagc caacagcttc
      181 gtcaccgggc agacgctgtt tgtctgcggt ggcgccagcg tgagctcgat caccatctga
      241 acgactggtg cggagcacca gagaccatac ggcgtcaaga caatgtttag atataaacta
      301 tcttcgctat gcacggatcg atgccggtgg ccatcggggg atgcaacaca cggctctccg
      361 cccacctgcc cgctagaact gtaccgccac tagcgtgaac agcctctaaa attcattttt
      421 cccacgacac acgaagaaga tcaggagaca gccacatgaa actcaagacc ctcgccacca
      481 ctgtggcggc gctcaccctg ctgggcacca ccgccgcctt cgcccaggtg aaaatcggcg
      541 tggtgtcctc cacgacggga cccacggcgc tgatcggcat tccccagaag aacaccgtgg
      601 cgctgctgcc gaagaaggtc ggcgatctga ccatccagta catttcgctc gatgacggca
      661 gcgaccccac caagaccgtc gttgatttca agaagctcat cagcgaagac aaggtggacg
      721 ccatcatcgg gccctcgggc tcgcccaacg ccatgggcgt gatccagttc gccgcccaat
      781 ccggcacgcc catgctggcg cccgtgggta cggcatcggt cgtgctgccc atgacgcctg
      841 aaaagaaatg ggtgttcaag accacgcaga acaccgacat catcgccaag gcgctgatcg
      901 gccacatggt caaggcgggg gtcaagaccc tgggcttcat cggcctgaac gacgcctacg
      961 gcaccgactg gctgaacgtg atcagcaaga tggccaaggc ccacaatatc aagatcgtcg
     1021 ccgtcgaacg ctatcagcgt tccgacagtt cagtcaccgg ccaggcgctg cgcgtgctcg
     1081 ccgccaagcc cgatgccgtg ctggtggccg gcaccggcgc ggcggccgtc ctgccggaga
     1141 ccacactggt caagcaaggc tacaagggcc agttctacca gacccacggc gcggccctgc
     1201 ctgcgttcct gaagctgggt ggcaaggcgg tcaacggcac cattctcgcg gccagcctga
     1261 tgctggtgct gccggaaatc tccgacagca acccgtcgaa gaagatcgct gaacaatacg
     1321 acgcaagcta tgaaaagctg tatggccaca agccggccac gttcggcgcc aacgtgtatg
     1381 acgcgggcct gttgctggag aaagccattc ccaaggccga gaaaaaggcc aagcccggca
     1441 ccaaggaatt ccgcagcgcc ctgcgcgacg ccctggagca aacccacgaa ctggtcgcca
     1501 cgcagggggt ctacaacatg acccccgaag accacagcgg gttcgacgat cgcggccgcg
     1561 agctgatcat cgtgaagaac cagcagtggc atttgttgaa gtgatgccta tcggccggac
     1621 ggcgaagtaa ccgttcggcc gaatccgccc tggcacaacg tcagccgacc gcttgcaaac
     1681 ttcaaacact gggcatgaaa ggcctggcgg gtcagctgac agctttcagg cgctccgcat
     1741 gaaaggccaa atgatcttcc ataaaggtcg acagaaaata atacccgtgg tcatagcctt
     1801 cgtgccgtcg cagcgtgagt ggctgcccgg ccttggcgca tgccgcttcg aattcgcccg
     1861 ggtacagttg ctcatcgaga aaggcatcgg ccaacccctg gtcgaccaga atgccgcccg
     1921 gatacgggca attggatcgt gccatcaata gcgaagcgtc gtgctcggcc caggaagcat
     1981 gatcatcacc cagatagccc gtgaacgcct tttgccccca cgggcaacgc gatggcgcgg
     2041 caatgggcgc gaacgccgac acggaacgat attgatcccg gtggcgctgc gccagcacca
     2101 gggctccatg gccgcccatc gaatgcccga aaatgccgac ccggcccgca tcacccggca
     2161 actcaccggt ggcaatgtcg aatagctcgc gggccacata gctttccatg cggtagtgcg
     2221 tcttccatgg agcctgcgtc gcgtccagat agaaccccgc gcctgtgccg aagtcccagt
     2281 cgtcctcatc acccgggacg ccggccccac gaggactggt gtcgggcgac accagcatca
     2341 acccatgctt cgcggcaagg cgttgcgcac ccgccttgac catgaagttc tcctcggtgg
     2401 cggtgagccc cgccaggaag aacagcaccg gtacgcgccc tgccgccgcc tgcggcggca
     2461 ggaacaccga gaatcgcatg ggcaggccaa tggcctgtga atcgtgacga taaaagcgct
     2521 ggacaccatc gaaacagcgg tgttcgctgg tcagttcgag tccgcccggc atcagtacac
     2581 caccacggaa cgaatggact caccgcgatg catcagatcg aacccgtcat tgatcttttc
     2641 gagcggcagg gtgtgcgtga tcaggtcgtc gatgttgaga cggccctcca tgtaccagtc
     2701 gacgattttt ggcacgtcag tgcgcccgcg tgcgcccccg aacgccgatc ccttccagtg
     2761 acgccccgtc acaagctgga agggacgagt ggaaatctcg gcgccggcct cggccacgcc
     2821 gatgatgatg gattgccccc agcccttatg gcaacattcc agcgcctgac gcatgacctt
     2881 gacgttgcct atgcattcga acgagtaatc ggcgccaccg tcggtcagcg acacgatatg
     2941 gtcgaccacg ttgtcgacgt cctttgggtt gacaaagtgc gtcatgccga acttgcgcgc
     3001 cagggcttcg cgcccggggt tcagatccac cccgatgatc ttgcccgcgc ccaccatctt
     3061 cgcgccctgg atgacgttca atccgatgcc gcccag
//
LOCUS       DASTNK010000153         7991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_324693, whole genome shotgun sequence.
ACCESSION   DASTNK010000153 DASTNK010000000
VERSION     DASTNK010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7991
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324693"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VFR20_11670"
     CDS             <1..1086
                     /locus_tag="VFR20_11670"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEU0231224.1"
                     /translation="QSNGQVNLPVNNFVRFTWRTVVPTAAHGPQAISVERPGAGMMAL
                     DVGDATSTGGSIAGNDKARTGGRASGFISAATTTNAPMPTTDLRTAAPGNAGSASNPV
                     PNHTDTSGQPSQTVRVEHSAFDNFRSAISTFEPIYFDFGDKGGANARFQLSFKYRLFT
                     PEDPRKPGFLDNLYFGYTQTSLWDLHDNSVPFVDTTYNPSLFWHKQKLWQTTDQRWFT
                     GLATGVEHKSNGKGGDDSRSLNDVFVQPELNHRFNNGSTLTFAPRVKAYFLDDQNADY
                     ASYLGRVDWKLRWAQDNGLVLDALYQQGHHGHHAVQLEAAWPLQRTFLHMNGYLHVQF
                     YQGYGETLLGYNVKADPQVRIGLSLVP"
     gene            1228..4791
                     /locus_tag="VFR20_11675"
     CDS             1228..4791
                     /locus_tag="VFR20_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEU0231225.1"
                     /translation="MNAPLDQAALSRLAKASLDDKYTLERGRIYISGTQALVRLPMLQ
                     RLRDRGAGLNTAGFISGYRGSPLGALDMALWKAREHLDANGIVFQPGLNEDLAATSVW
                     GTQQINLYPDATVDGVFGMWYGKGPGVDRSMDVLKHANSAGTSPHGGVLVLAGDDHAA
                     KSSTLAHQSEHVFQAAGIPVLYPANVQEYLDYGLHGWAMSRYSGLWVAMKCVTDVVES
                     TASVDIDPDRVRIVLPEDVALPDGGLGIRWPDPPLVQEARLMDHKWYAALAYTRANRL
                     NRVVIDSPRARFGIMAAGKAYLDTRQALADLGLDEATCAQIGIRLIKIGCVWPLNAQD
                     AREFATGLEEILVVEEKRQILEYALKEELYNWRDDVRPRVYGKFDEKDNAGGEWSTPR
                     GQWLLPAHSELSPAVIAKAIGRRLDKAGLPADIGAHIAARLAIIDAKERELAGPVTTV
                     ERKPWFCSGCPHNTSTKVPDGSRAMAGIGCHYMAIWMDRNTATFSHMGGEGAAWIGQA
                     PFTREKHVFANLGDGTYCHSGLLAIRAAVSAGVNITYKILYNDAVAMTGGQPVDSGLK
                     VSDLVAQVHAEGVRRVVIVTDEPEKYHGIALVGQPTVHHRRDLDKIQRELRDIAGTTV
                     LVYDQTCATEKRRRRKRGAYPDPARRVFINPAVCEGCGDCSVQSNCLSVEPLETPLGT
                     KRKINQSSCNKDFSCANGFCPSFITAEGAQVRKPSMDAGRTASLDSFNLPEPALPALD
                     TGYGILVTGIGGTGVVTIGGLLGMAAHLEDKGVTVLDMAGLAQKGGAVLSHVQIANRP
                     EDIHATRIATGEAALVIGCDAIVSSSAEVLSKVREGVTRAAINSADSPTADIIRNPKW
                     TFPRASTEAHLEQAIGAGCEFLDANALAVRLLGDAIYANPLMLGYAWQKGWIPLSLES
                     LMRAIELNGVQIENNKNAFTWGRTVAQHGAQAVAPEPRPSGGDADVTALPETLDALVA
                     RHIKQLTAYQNKRYARRYARAIEKIRAKEASLDAPGALPLTRAVAVNLAKLMAYKDEY
                     EVARLFTDPVFKEQLREQFQGEPGKDYQVHFHLAPPLLARKDESGHLIKRKFGPWMMR
                     AFGILARCKGLRGTPLDVFGKTDERRMERQLIVDYIDLVEELDRTLDQGNRDTAIALA
                     SLPDDIRGFGHVKDKSIEVVAVRRASLLERYRSTAPVRQAA"
     gene            4797..6710
                     /locus_tag="VFR20_11680"
     CDS             4797..6710
                     /locus_tag="VFR20_11680"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEU0231226.1"
                     /translation="MRDLTLAFIVSSFCLLTLSRCLLAAWQWPRVRQAGGLLPILRGG
                     LRIDAHQIAIFAAVPALLSPWLGHMQAAVSITALWFQACWFIFVLLEASTPQFIQEYD
                     TRPNRLYVEYLKHPQEVFGMLWKGYKAVILAALGVLAAFVWLGHGLFGHIAPDTAMAW
                     WLRPVVSLVVVAVAILAIRGSLGHRPINPSTVAYCGDSMLNTLPLNSFYNVCYALYSM
                     KNERSAHDVYGDMPDEDVNDIVTRCAGMPRTHSDIPTLHLQQPITERARPLNIVMIIE
                     ESLGAQYVSHLGGVELTPELDALAAQAWTFTRAFASGTRSVRGLEAVVAGFPPTISDA
                     ALRLPDAQSNFFTLAQTLRRHGYRSRFIYGGEAHFDNMKSFFLGNGFDDLYDLPTFSN
                     PAFVGTWGVSDEDMCDRLDDLLSKDGEQPTFTLAFSVTNHSPWEYPRGRIESQGEPAS
                     EQNTVRYADWALGRFFRKARESQYWDNTIFLIVADHDSRVFGANLVPLKHFHIPALIL
                     GGTVEPRRDDRLISQIDLPPTLLSLAGLRTEHPMIGHDLTRPGNGPGRAMMQYNENYG
                     YLRGDNLLVLEPHRPPTQYTWRAPDDYTPVDIDPDLAREALAHVLWPSWAYRNRAYTL
                     PGLGRSGGGRASA"
     gene            6905..7918
                     /locus_tag="VFR20_11685"
     CDS             6905..7918
                     /locus_tag="VFR20_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEU0231227.1"
                     /translation="MTTDHPSTPAHDISTSPAVEPALTAGAAAIRNVAVIGAGTIGAS
                     WAALFLAHGLNVTMCDPATNAEASARTLIGQAWPTLAALGVARGDVPWTALRMDANLD
                     TALADTEFVQENAPEREDFKIDLFARMDAAAPPHAILASSTSGLIMSRLQSRCEHPGR
                     CVIGHPFNPPHLIPLVEVVGGEKTSSDTILRAMAFYRRVGKHPIKINKEVTGHVANRL
                     QAAVWREAIHLAMEGVASVEDIDAAMAYGPGLRWAVFGPHMTFHLGGGQGGMGHFLDH
                     LKGPVQSWWDDLGDARLDDATRRALLDGLEQESRGRTINDLARERDTAIIRILAAKKQ
                     AGV"
BASE COUNT         1525 a         2717 c         2418 g         1331 t
ORIGIN      
        1 cagagtaacg ggcaggtcaa tcttccggtg aataacttcg tgcgtttcac ctggcggacg
       61 gtggtgccca ccgcggcgca cggcccacag gccatcagcg tcgaaagacc gggtgccggc
      121 atgatggcgc tggacgtggg cgatgcgacg agcacgggcg ggtccatcgc gggcaacgat
      181 aaagccagga caggcggccg tgcatccgga ttcatcagcg cggccacgac caccaacgcg
      241 ccaatgccaa ccaccgactt gcgcacggcc gcgccgggga atgccggcag tgcttcaaat
      301 cccgtcccga accacactga cacatccggg cagccatctc aaacggtacg ggtcgaacac
      361 tcggccttcg acaacttccg cagcgcgatc tcgacgttcg agccgatcta cttcgatttc
      421 ggcgacaagg gtggtgcgaa cgcgcgcttc cagctcagtt tcaagtaccg gctgttcacg
      481 cccgaggacc cgcgcaagcc cggatttcta gacaatctgt acttcggcta cacgcaaaca
      541 tcgctgtggg atctacatga caattcggtg ccgttcgtcg acaccaccta caatcccagc
      601 ctgttctggc acaagcaaaa gctctggcag acgaccgatc aacgctggtt cacggggttg
      661 gccaccggcg tggaacacaa atcgaacggc aagggcggcg acgattcacg ctcactcaac
      721 gacgtcttcg tgcaacccga gctcaatcat cggttcaaca acggcagcac actgaccttc
      781 gccccccgcg tcaaggccta tttcctcgat gatcaaaacg ccgactacgc atcctatctg
      841 gggcgcgtcg actggaagct gcgctgggcg caggacaacg ggctggtgct ggatgcgctg
      901 tatcagcagg gccaccatgg gcatcacgcg gtccagctcg aggccgcgtg gcccctgcaa
      961 cgcacgttcc tgcacatgaa cggctacctg cacgtgcagt tctatcaggg atacggggaa
     1021 acattgctgg gctacaacgt caaggccgac ccccaggtac gcatcggcct gtcgctggta
     1081 ccctgattcc ggacgcacgg cgtgattttg cggggcgtgg attagctcat acactagggc
     1141 tacccactcg aaaggccccg cacaacgagg gcctggctag ggcagacccc acacggcgca
     1201 aacgaccgga cacgcaggag acttgagatg aacgcacccc ttgatcaggc ggcgctatcc
     1261 cggcttgcca aagcctccct tgacgacaag tacacgcttg aacgcggtcg tatatacatc
     1321 agcggcacgc aagcgctggt gcgcctgccc atgctgcagc gcctgcgcga ccgcggggcc
     1381 gggctcaaca cggcgggctt catctcgggc taccgcggct ccccccttgg cgcgctggac
     1441 atggccttgt ggaaggcccg ggaacacctg gacgccaacg gcatcgtgtt ccagcccggg
     1501 ttgaatgaag accttgcggc cacatccgtt tggggcacac agcagatcaa cctgtatccg
     1561 gacgccaccg tcgatggggt gttcggcatg tggtacggca agggccctgg cgtggaccgg
     1621 tccatggacg tgctcaagca cgccaactcg gccggcacct caccgcatgg cggggtgctg
     1681 gttctggcgg gggatgatca cgccgccaag tcatccacgc tggcgcatca gtccgaacac
     1741 gtgttccagg cggccggcat tcccgtgctg taccccgcca acgtacagga atatttggac
     1801 tacggcctgc acggctgggc catgagccgg tattcgggcc tgtgggtagc catgaagtgc
     1861 gtcaccgacg tcgtcgaatc caccgcgtcg gtggatatcg acccggaccg cgtccgtatc
     1921 gtcctgccgg aagacgtcgc cctgcctgac ggggggctgg gcatacgatg gcccgacccg
     1981 ccactggtgc aggaagcgcg cctgatggat cacaagtggt acgcggcgct ggcctacacg
     2041 cgcgcgaatc ggctcaatcg cgtcgtcatc gattcaccca gggcgcgctt cggcatcatg
     2101 gcggccggca aggcctatct ggacacgcgc caggcgctcg ccgatctggg gctggacgag
     2161 gccacctgcg cgcaaatcgg catccgtctc atcaagattg gctgcgtgtg gccgctcaac
     2221 gcccaggacg cccgcgaatt cgccaccggg ctggaggaaa tcctggtcgt ggaggaaaag
     2281 cgccagatcc tcgagtacgc gctcaaggaa gaactgtaca actggcgcga cgatgtacgc
     2341 cccagggtgt acggcaagtt cgacgaaaaa gacaatgcgg gcggtgaatg gtccacaccg
     2401 cgcggccagt ggctgttgcc cgcgcacagc gagctttccc ccgccgtcat cgccaaggcg
     2461 atcggccgtc gcctcgacaa ggctggcctg ccggccgaca ttggcgcgca catcgccgcg
     2521 cggctggcca tcatcgacgc caaggaacgc gaactggccg gtcccgtcac cacggtggaa
     2581 cgcaagccgt ggttctgctc cggctgcccg cacaacacct ccacgaaggt cccggatggc
     2641 tcacgggcca tggccggcat cggttgccat tacatggcca tctggatgga ccgcaacacc
     2701 gccacgttca gccacatggg cggcgaaggc gcggcctgga tcggccaggc ccccttcact
     2761 cgcgagaaac atgtgttcgc caacctgggc gatggcacgt actgccattc gggcctgctg
     2821 gccatacgcg ccgctgtgtc cgcgggcgtc aacatcacct acaagattct ctacaacgac
     2881 gccgtggcca tgaccggtgg ccagccggtc gacagcgggc tcaaggtttc cgacctggtg
     2941 gcgcaagtgc atgccgaggg cgtgcgacgc gtcgtcatcg tgaccgatga gcccgaaaag
     3001 tatcacggca tcgcgctggt gggtcagccc actgtgcatc accgccgtga tctcgacaag
     3061 atccagcgcg aactgcgcga catcgcgggc acgaccgtgc tcgtctacga ccagacgtgc
     3121 gccaccgaga agcgccgccg gcgcaagcgc ggcgcctacc ccgaccctgc ccgccgcgta
     3181 ttcatcaatc cggcggtctg cgaaggctgc ggcgactgct cggttcaatc caactgcctg
     3241 tcggtcgaac ccctggaaac gccgctgggc accaagcgca agatcaacca atcgtcatgc
     3301 aacaaggatt tttcctgcgc gaacggtttc tgccccagtt tcatcaccgc cgagggcgcg
     3361 caggtgcgca aaccctccat ggacgccggc cgcacggcca gcctggattc tttcaacctg
     3421 cccgaacccg cgttgccggc cctcgatacc ggctatggca ttctggtcac cggcataggc
     3481 ggcaccgggg tggtcaccat cggcggcctg ctcggcatgg cggcgcacct ggaggacaag
     3541 ggggtcaccg tgctcgacat ggccggcctc gcccagaaag gcggtgccgt gctcagccac
     3601 gtgcagatcg cgaaccggcc ggaagacatc cacgccacgc gcatcgccac aggcgaagcc
     3661 gccctggtca tcggttgcga cgccatcgtg tcgtcgtcgg ctgaagtcct gtccaaggtc
     3721 cgcgaagggg tgactcgcgc cgccatcaac agcgccgaca gccccaccgc cgacatcatc
     3781 cggaatccaa aatggacgtt cccgcgcgcg tcgaccgaag cccacctgga acaagccatc
     3841 ggcgcgggct gcgaattcct cgacgccaac gcgctggcgg tgcgattgct gggcgacgcc
     3901 atctacgcca acccgctgat gctgggctat gcatggcaaa agggctggat acccctgtcg
     3961 ctggaaagcc tgatgcgcgc catcgaactc aacggcgtgc agatcgagaa caacaagaac
     4021 gcgttcacat ggggccggac agtggcgcag catggtgcgc aggctgtggc gcccgaaccg
     4081 cgcccatccg gcggggacgc cgacgtgacg gccctgcccg aaacactgga cgcgctggtg
     4141 gcgcgccata tcaaacagct gacggcctac cagaacaaac gatacgcgcg gcgatacgcg
     4201 cgcgcgattg aaaagatacg cgccaaagag gcgtcgctgg atgcccccgg cgccctgccg
     4261 ctgacccgcg ccgtggctgt caatctcgcc aagctcatgg cctacaaaga tgaatacgaa
     4321 gtcgcgcgcc tgttcaccga cccggttttc aaggaacaac tgcgtgaaca gttccagggc
     4381 gagcctggca aggactatca ggtgcatttt catctggccc cgccgctgct ggcccgcaag
     4441 gatgaaagcg gacacttgat caagcgcaag ttcggcccct ggatgatgcg cgcatttgga
     4501 atcctggccc gatgcaaggg tttgcgcggg acgcctctcg atgtctttgg gaaaacggac
     4561 gaacggcgaa tggagcggca actgatcgtt gactacatcg atctggtcga ggagcttgac
     4621 cgcaccctgg atcagggcaa tcgcgacacg gccatcgcac tggccagcct gcccgacgac
     4681 atacgcggct ttggccacgt gaaggacaag agcatcgagg tggtggcggt tcggcgtgcc
     4741 tcactcttgg agcgatatcg gagcacggca ccggtgcgac aagctgcctg acccctatgc
     4801 gcgacctcac cctggccttc atcgtttcca gcttttgcct gctgacactc agccgctgcc
     4861 tgctcgcggc atggcagtgg ccgcgcgtcc ggcaggcggg tggactgctg cccatcttgc
     4921 gaggcggatt gcgcatcgac gcgcaccaga tcgccatctt cgcagccgtc ccggcgctgc
     4981 tttccccctg gctcggtcac atgcaggcgg cggtctccat caccgccctc tggttccagg
     5041 cgtgctggtt catcttcgtg ctgctggaag cgtccacacc ccaattcatc caggaatacg
     5101 acacccgccc caaccgcctg tacgtggaat acctgaagca tccacaagaa gtcttcggca
     5161 tgctgtggaa aggctacaag gccgtcatac tcgcggcgct cggggtgctc gcggcgttcg
     5221 tgtggctggg gcacgggctg ttcggtcaca tcgcgccaga cacagccatg gcatggtggc
     5281 tgcgccccgt cgtcagcctg gtggtggtgg ctgtggccat tctggccata cgcggctccc
     5341 tgggccaccg ccccatcaac ccttcaaccg tcgcatattg cggcgacagc atgctgaaca
     5401 ccttgccgct gaattcgttc tacaacgtct gctatgcgct ctacagcatg aagaatgagc
     5461 gctcggcgca cgatgtgtat ggcgacatgc ccgacgaaga cgtcaacgac atcgtcacgc
     5521 gctgcgcggg aatgccgcgc acgcactctg acatccccac cctgcacctg cagcaaccca
     5581 tcacagagcg cgcccgcccc ctgaacattg tcatgatcat cgaggaaagc ctgggcgcgc
     5641 agtatgtcag tcatctgggc ggggtggaac tcaccccgga actggatgcg ctcgccgccc
     5701 aagcctggac gttcacccgg gccttcgcca gcggcacccg ttcggtgcgc ggcctggagg
     5761 ccgtggtggc ggggttccca cccacgatct ccgatgccgc cctgcgcctg ccggatgcgc
     5821 aaagcaattt cttcacattg gcgcagacgc tccgccgaca cggctatcgt tcccgcttca
     5881 tctacggcgg ggaagcccat ttcgacaaca tgaagagctt cttcctgggc aacggtttcg
     5941 acgacctgta cgacctgccc acgttctcca atccggcttt cgtcggcacc tggggggtat
     6001 cggacgaaga tatgtgcgac cgcctggacg acctgctttc caaagatggg gagcagccca
     6061 ccttcacgct ggccttttcc gtcaccaatc attcgccatg ggaatatccg cggggccgca
     6121 ttgaatccca aggggagccg gccagcgagc agaacacggt gcgctacgcc gactgggcgc
     6181 tcggacggtt cttccgcaag gcgcgggaat cccaatactg ggacaacacc atatttctca
     6241 tcgtcgcgga ccatgattcg cgcgtgttcg gcgccaatct ggtgcccctc aaacatttcc
     6301 acattcccgc cctgatcctc ggcggcaccg tcgagccgcg ccgggacgac cgcctgatca
     6361 gccagatcga tctccccccc actttgctgt cgctcgcggg cctgcgcacc gagcacccca
     6421 tgatcggcca tgacctcacc cggcccggca atggccccgg gcgcgccatg atgcagtaca
     6481 acgagaacta cgggtatctg agaggtgaca acctactggt cctcgagccg catcgcccgc
     6541 ctacgcagta cacgtggcgc gcccccgacg actacacgcc cgtcgacatc gatcctgatc
     6601 tggcgcgcga agccctggcc cacgtgctgt ggcccagttg ggcgtaccgg aaccgcgcct
     6661 acacgctgcc agggctgggc cgttcgggcg gcgggcgggc ttcggcctga agaacaggct
     6721 cgtccgagcc tggttgcggt gggccgggtc acatcgcgtt gatccgagcc tggttgctct
     6781 gacccgagac ttgccacttt gatcagagct gcgtctcagt gccttgcgtc cgatgaattc
     6841 tcgcaaagca cgatgcgtac aatacctggt gacaaagtca cggaaccatc aacgggagcg
     6901 tcgcatgacc acggatcacc cttcaacccc ggcacacgac atctccacca gcccggctgt
     6961 cgagccggct ctgacagccg gcgccgccgc tatccgcaac gtcgccgtca tcggtgccgg
     7021 aaccataggg gccagctggg ccgccttgtt tctggcccac ggcctgaacg tcacgatgtg
     7081 cgaccccgcc accaacgccg aggcatcggc acgcaccctc attggccagg cgtggccgac
     7141 gctggcggcg ctcggtgttg cgcgcggcga cgtgccctgg acagccctgc gcatggacgc
     7201 caatctcgac accgccctgg ctgatactga attcgtccag gaaaacgccc cggaacgcga
     7261 agacttcaag atcgatctgt tcgcccgcat ggacgccgcc gcgccaccac acgccatcct
     7321 ggcatcaagc acgtccggct tgatcatgag ccgcctgcag agccgctgtg aacaccccgg
     7381 ccgctgcgtc atcggccatc cgttcaaccc accccacctc atcccgctgg tcgaagtcgt
     7441 ggggggtgag aaaacctcat ccgacaccat cctgcgcgcc atggcattct atcggcgcgt
     7501 gggcaagcat cccatcaaaa tcaacaagga agtcacgggc catgtcgcca accgcctgca
     7561 ggcggctgta tggcgcgagg ccatccattt ggccatggag ggcgtggcat ccgtcgaaga
     7621 catcgatgca gccatggcct acggccccgg cctgcgctgg gcggtgttcg gcccacacat
     7681 gacattccac ctgggcggcg gacaaggtgg catgggccat ttcctggacc acctgaaagg
     7741 ccccgtgcaa agctggtggg atgatctggg tgatgcgcgc ctggatgacg ccacgcgccg
     7801 cgcgctgctg gacggactgg aacaggaaag ccgcgggcgc accatcaacg atctcgcccg
     7861 cgagcgcgac acggcgatca tccggattct ggcggcgaaa aaacaggcgg gtgtttgaag
     7921 cccgctggga tgtcacaccg acaaactcca tggcccggat cacgtaccgc ccagcccgat
     7981 tcatcgtcgt c
//
LOCUS       DASTNK010000154         6842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_509872, whole genome shotgun sequence.
ACCESSION   DASTNK010000154 DASTNK010000000
VERSION     DASTNK010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6842
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509872"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /gene="bamA"
                     /locus_tag="VFR20_11690"
     CDS             <1..1189
                     /gene="bamA"
                     /locus_tag="VFR20_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEU0231228.1"
                     /translation="YDAASIKQSRDRIDRLGYFNDVNVHTTPVPGSPDQVDVDVNVKE
                     KPTGLINLGIGYGTTDKVMLSAGISQDNVFGSGKSLSLQVNTSQINRAAVLSSTDPYW
                     TKDGISKTTSLYYRRTSPYLTSSNSGDFRVTAMGLGLNFGVPISQYDRVFMGARYEQN
                     RIDLLGNPPQAYTDFVNAYGPKTNSVIFNVGWSKDTRDSALAPTKGSYTHLSTDVSTM
                     NLKYYMLSGQQQWYIPLSRSFTLALNGQFDWGRAYGGKPFPIIKTVYAGGIGSVRGYQ
                     GASLGPRDTVTGNYLGGTRRIVGNVQLYLPFPGASRDRTLRWFVFADAGQVSDAGGSG
                     LTCTSSASSPVQDPCGWRYSAGLGLSWQSPMGPLQLSWGRALNAKPGDDKQAFQFQIG
                     TGF"
     gene            1318..1806
                     /locus_tag="VFR20_11695"
     CDS             1318..1806
                     /locus_tag="VFR20_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEU0231229.1"
                     /translation="MAFAAVLGLSVAMVAPAHADGTKIGFVSTERILRDSKPAKAAQA
                     KIEAEFKGRDAALQKMAADLRAKAQKLDKDAPVLSQAELTKRQRALADMDSNLQRKRR
                     EFQEDFNRARNEEFSSIVAKANAAIKKIAEQQHYDLIIQDAVTVSPKVDITDQVLKAL
                     GG"
     gene            1813..2913
                     /gene="lpxD"
                     /locus_tag="VFR20_11700"
     CDS             1813..2913
                     /gene="lpxD"
                     /locus_tag="VFR20_11700"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEU0231230.1"
                     /translation="MPVLLAPEQAPALHDLLAQANTAQLDVDVIAGRRGANPAIRGLG
                     SLLGAGPGEISFLADTRLAHQLKDCAASAIVMTAESYDTLHADQPTCCVVLCSQPYLM
                     YALLAQWFDRQRVAGLDAGVHPSAVVAAGAHLDDDVGLGPHCVVEEGARIGRGSRLGA
                     GVTVGANSVIGPDCLLYSHVTLYHGVRMGARVIVHSGAVLGADGFGFAPDPRATNGGW
                     AKIAQLGGVVVGDDVEIGANTTIDRGAIDDTIIGNGVKLDNQIMIAHNVKVGENTAMA
                     ACVGIAGSTTIGRRCTIGGAAMISGHLVLADDVHVSGGTAITSNLDTPGRYTGVYPFA
                     EHSQWQRNAAVVSQLSQLRRRLRVLEKQGRTD"
     gene            2935..3387
                     /gene="fabZ"
                     /locus_tag="VFR20_11705"
     CDS             2935..3387
                     /gene="fabZ"
                     /locus_tag="VFR20_11705"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU0231231.1"
                     /translation="MKLDIKEIMARIPHRYPMLLVDRVLDMVPGKSIVAIKNVSIDEP
                     FFTGHFPHNPVMPGVMIIEAMAQAAALFSFENDHDVNPADKKIAYYLVGVDGARFRRP
                     VVPGDQLRLEVVADRISRSICKYTGVATVDGQEVASAKLMCAIRVLED"
     gene            3392..4186
                     /gene="lpxA"
                     /locus_tag="VFR20_11710"
     CDS             3392..4186
                     /gene="lpxA"
                     /locus_tag="VFR20_11710"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEU0231232.1"
                     /translation="MAGAIHATAIVAPGAKLADGVSVGPYSVIGEHVSIGAGSVVGPH
                     CVIDGHTAIGENNTFYRFCSIGGIPQDKKYAGEPTRLEIGHGNTVREYVTINTGTAQD
                     VGVTRLGDDNWIMAYVHIAHDCQIGSHTVIANSVQLAGHIHIDDWAIIGGLTGSHQFV
                     RIGAHTMIGGTSSIRQDIPPYVIGAGDPFRPVGINAEGLSRRGFEPDVIATLKEAYKI
                     LYRRQLGVEQAVEHIRALQESHSSAQAALQVLVDFLDASTRGIARG"
     gene            4183..5409
                     /gene="lpxB"
                     /locus_tag="VFR20_11715"
     CDS             4183..5409
                     /gene="lpxB"
                     /locus_tag="VFR20_11715"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEU0231233.1"
                     /translation="MTPRIGLVAGEPSGDLLASRIMHGLIQRAEPALCAGIGGPAMAA
                     QGLETWHSMHALTVFGYVDAFKRLPGLLGTYFDVRRRWLAQRPDVFVGIDAPDFNLRL
                     ERVLKQAGIPTVHFVGPSIWAWRYERIHKIRAAVSHMLVLFPFEEALYQREGVPVTYV
                     GHPLASAIPAMPDRQAVRRFLGIDEGARVLAMMPGSRASEIRLLAPRFLQAVRLLQAD
                     DPALSVLVPMVNAQRRAEFEAILRRNPISNCRILGGPEPGDGLAPGGKRESVGAGYDD
                     RPAAWNAMEAADAVLVASGTATLEAALFKRPMVISYVLSPMMRRLMAWKSGQSRPYVP
                     WVGLPNVLLNDFAVPELLQDDATPEALAKATQAALYDSSLAEHLRRRFTGLHADLTRD
                     TAGLAAKVILEQAGAA"
     gene            5406..6020
                     /gene="rnhB"
                     /locus_tag="VFR20_11720"
     CDS             5406..6020
                     /gene="rnhB"
                     /locus_tag="VFR20_11720"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU0231234.1"
                     /translation="MSQAGLFDQPPPVLTAGVDEAGRGPLAGDVFAAAVILDPELPIP
                     GLADSKVLSASRREHLAGLIRERALAWHIASASVEEIDALNILQATMLAMRRACAGLN
                     PAPELALIDGNRVPRPLPCEARAIVKGDALVAAISAASILAKTARDAYCASMHQAYPQ
                     YAFDQHKGYGTALHLARLREHGPCPTHRRSFGPVKRLLGAESQP"
     gene            6017..6796
                     /locus_tag="VFR20_11725"
     CDS             6017..6796
                     /locus_tag="VFR20_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU0231235.1"
                     /translation="MKRISSRENPAYKRVLRIAEGKERGWAVIEGIHLCRAWLDHVGQ
                     PDMALFDEGGLGPDGEQAGLASRLDAHRCLVCEPRSWRAVSGVEHGQGVLFVVSVPEP
                     DIPLTLDRNCIWLDRVQDPGNLGTLLRTAAAAGIGQAYLSEGCANAWSQKVLRSAQGA
                     HFVMAIHERQELRALRARLRIPLVATSLRDARSIDDVALPADCAWLFGNEGRGVADDL
                     LDAADERVFIPQVATVESLNVAAAAAICLFEQRRRHRVGAA"
BASE COUNT         1218 a         2129 c         2198 g         1297 t
ORIGIN      
        1 gtacgacgcg gccagcatca agcagtcgcg cgatcgcatc gatcgcctgg gttatttcaa
       61 cgatgtcaat gtacacacga cgcccgtgcc cggttcgccc gaccaggtcg acgtcgatgt
      121 caatgtcaag gaaaagccca caggcctcat caatctgggt attggttatg gcaccaccga
      181 caaggtgatg ctgtcggccg gcatcagcca ggacaacgtc ttcggcagcg gcaagtcact
      241 gtcgttgcag gtcaacacca gccagatcaa tcgcgcggcg gtgctgtcgt ccaccgatcc
      301 ctattggacc aaggatggca tcagcaagac gacgtcgctg tattaccgac gcacttcgcc
      361 ttacctcacc agcagcaata gcggcgattt tcgcgtcacg gccatggggt tggggcttaa
      421 tttcggtgtc cccatttccc agtacgaccg cgtcttcatg ggcgcacgtt acgagcagaa
      481 caggatcgat ctgcttggca accctcccca ggcctatacc gattttgtca atgcctacgg
      541 cccaaagacg aattcagtca tcttcaacgt aggctggtcc aaagacacgc gcgacagcgc
      601 attggcgcca accaagggca gctacacgca tttgtccacc gacgtgtcca cgatgaacct
      661 gaagtactac atgctcagcg ggcagcagca gtggtacatc ccgttgtcgc gcagcttcac
      721 gctggcgtta aacggccagt tcgattgggg tcgggcctac ggtggcaaac cattcccgat
      781 catcaagacg gtatatgccg gcggcatcgg ctctgtgcgg ggctatcagg gggcgtcatt
      841 ggggccacgc gacacggtaa ccggcaacta tcttggcggt acccgtcgca tcgtcggcaa
      901 cgtacagttg tatctgccgt ttccaggggc ctcgcgcgac cgcacactgc gttggttcgt
      961 cttcgccgat gccggccagg tgtcggatgc cggcggcagc ggcctgactt gcacctcatc
     1021 ggcctctagc cctgttcaag acccttgcgg ctggcggtat tcggccggcc tgggcctgtc
     1081 ctggcaatcg cccatgggtc cgttgcagct ttcctggggt cgcgccctga acgcgaaacc
     1141 gggcgacgac aagcaggcat ttcaattcca gatcggcacc ggtttttgat ccgggccgtt
     1201 taatggcaaa atagctcttt ttatctctgc aattcgcaag gtagattctt catgacgtcc
     1261 cagatcgcat cgaaactctc cggttacacc cgtctggccg gccgttacgg caatgccatg
     1321 gcattcgccg ccgtgcttgg ccttagcgtg gcaatggtcg ccccggcgca tgccgacggc
     1381 accaagatcg gttttgtcag cacggaaagg attctgcgcg actccaagcc cgccaaggcg
     1441 gcccaggcca agatcgaggc ggaattcaag ggccgcgacg cggcactgca aaaaatggcg
     1501 gccgatctgc gcgccaaggc ccagaagctc gacaaggacg caccggttct ttcccaggct
     1561 gaactcacca agcgccagcg cgccttggcc gacatggatt cgaatcttca acgcaagcgc
     1621 cgcgaattcc aggaggactt caaccgcgcc cgcaacgagg aattctcaag catcgtcgcc
     1681 aaggccaacg cggccatcaa gaaaatcgcc gagcagcagc attacgacct gatcatccag
     1741 gacgccgtca ctgtcagccc caaggttgac atcacggatc aggtcctcaa agcgctgggt
     1801 ggttaaaaac ccatgcccgt gttgctcgcg ccggaacagg cgcctgccct gcatgacctg
     1861 ttggcgcaag caaatacggc gcagctcgat gttgatgtga tcgccggccg gaggggcgcg
     1921 aatccggcca ttcgcgggtt ggggtcgcta ctcggcgccg gccccggcga gatcagtttt
     1981 ctggcggata cccgcctggc gcatcagttg aaggattgcg ccgcctcggc catcgtcatg
     2041 accgccgagt cctacgatac cctccatgcc gaccaaccca cgtgttgcgt ggtgctgtgc
     2101 agccagccat acctgatgta cgcgctgctg gcgcagtggt tcgaccgaca acgtgtggcc
     2161 ggcctcgacg ccggtgtgca tcccagcgct gtggtggcgg ccggcgcgca tcttgacgat
     2221 gacgtcggtc tggggccgca ttgtgtcgtg gaggaaggcg cgcgtatagg ccgtggctcg
     2281 cgcctgggcg cgggggtcac tgtgggggcg aactcggtca ttgggcccga ttgcctgttg
     2341 tattcccacg tgacgctcta ccatggtgtg cgtatggggg ctcgcgtcat cgtgcattcc
     2401 ggtgcggtgc tgggcgccga tggcttcggc ttcgcgcccg atccccgtgc gacgaacggg
     2461 ggctgggcga agatcgcaca acttggcggg gtcgttgtcg gcgatgacgt cgaaatcggc
     2521 gcgaacacca cgatcgatcg tggcgccatc gacgacacca tcatcgggaa tggtgtcaag
     2581 ctcgacaacc agatcatgat cgcccacaac gtcaaagtcg gcgagaacac agccatggcc
     2641 gcgtgcgtcg gcatcgccgg ctcgaccacc atagggcggc gttgcaccat cggtggggcc
     2701 gccatgattt cgggccacct ggtgctggcc gatgacgtcc acgtatcggg cggcacggcc
     2761 atcacctcga atctcgacac gcccgggcgc tacaccggcg tgtatccttt tgccgagcat
     2821 tcccaatggc aacggaacgc ggctgttgtt tctcagcttt cgcagttgcg gcgccgcctg
     2881 cgggtacttg aaaaacaggg ccgcaccgac tagcccgagc aggaaaacaa atcaatgaaa
     2941 ctagacataa aagaaatcat ggcccgcatc ccgcatcgct atccgatgct gctggtcgac
     3001 cgcgttctag acatggtgcc gggcaaaagc atcgtggcta tcaagaacgt gtccattgat
     3061 gaaccgtttt tcaccgggca tttccctcac aacccggtca tgcctggtgt gatgatcatc
     3121 gaggccatgg cgcaagccgc ggccctgttt tcgttcgaga atgaccacga cgtcaatccc
     3181 gcggacaaga aaatcgctta ttacctggtg ggtgtggatg gcgcgcgctt tcgccggccg
     3241 gtcgtgccgg gcgatcaatt gcgcctcgag gttgtcgccg accgcatcag tcgctccatc
     3301 tgcaagtaca caggggttgc cacggtggac gggcaggaag tggcgtcagc caaactgatg
     3361 tgcgcgatcc gcgtgctgga agactgaccc catggccgga gcgatccatg ccacggctat
     3421 cgtggcgcca ggcgcgaagc tcgctgatgg cgtatccgtg gggccgtaca gcgtcatcgg
     3481 cgaacacgtc tccatcggtg cgggaagcgt ggtcgggccg cattgtgtca tcgacgggca
     3541 caccgccatc ggtgaaaaca acacgttcta tcgattttgc tcgataggcg gcatcccgca
     3601 ggataaaaaa tacgcgggcg agcccacacg ccttgaaatc ggccatggca acacggtgcg
     3661 ggaatacgtc accatcaaca ccggcacggc acaggacgtg ggtgtgaccc ggctcggtga
     3721 cgacaactgg atcatggcgt acgtgcacat cgcgcacgat tgccagatcg gcagccatac
     3781 cgtgatcgcc aattcggtgc agcttgccgg ccatattcac attgacgact gggcgatcat
     3841 cggtgggctc acgggctcgc atcagttcgt gcgcatcggg gcgcacacca tgatcggcgg
     3901 caccagttcc attcgccagg atattcctcc ctatgtcatc ggcgcgggag atccctttcg
     3961 ccccgtgggc atcaacgccg aggggttgtc gcgacgcggt ttcgagcccg atgtcatcgc
     4021 aactctgaaa gaggcctata agattctgta tcgacgccag ctcggcgtcg agcaggccgt
     4081 cgagcacatc cgggcgctgc aggaatcgca ctcgtcggcc caggccgcgc tgcaggtgct
     4141 ggtcgacttt ctcgacgcat cgacccgcgg catcgcgcgc ggatgactcc ccgcatcggc
     4201 ctggtggctg gtgagccatc gggggatctt ctcgcttcac gcatcatgca cgggctcatt
     4261 caaagggccg agcccgcgct ctgcgccggc attggcggcc cggccatggc ggcgcaagga
     4321 ttggaaacct ggcactccat gcatgcgctg acggttttcg gctacgtcga tgcgttcaaa
     4381 cgtctgcctg ggctccttgg cacatacttc gacgtcaggc ggcgctggct tgcccagcgg
     4441 cccgacgtgt tcgtcggcat cgacgcgccg gacttcaacc tgcggctgga acgtgtgctg
     4501 aagcaggccg gcatccccac agtgcatttc gtggggcctt cgatctgggc ctggcgctac
     4561 gagcgtatcc acaagatccg tgccgcggtg tcgcacatgc tggtgttgtt tccgttcgag
     4621 gaagcgctct atcaacggga gggcgtgccg gtcacttatg tgggtcatcc cctggcgtcg
     4681 gcgattcccg ccatgcctga tcgacaggcg gtccggcgtt tcctgggtat cgacgaaggc
     4741 gcccgggtgc tggccatgat gccgggcagc cgcgcttcgg agatccgcct tctggcaccc
     4801 cgttttctac aggctgtccg gttgctgcag gccgatgatc cggcgctgag cgtgcttgtg
     4861 cccatggtca acgcgcaacg acgcgccgaa ttcgaggcaa tactgaggcg aaacccgata
     4921 tccaattgcc gcatcctggg tggtccggaa ccgggtgacg ggttagcccc cggcggcaag
     4981 cgcgaatccg tcggcgcggg ctacgatgac aggccagctg cctggaatgc catggaagcc
     5041 gcggacgcgg tgttggtggc cagcgggacg gccacgctgg aagccgcgct cttcaagcgc
     5101 cccatggtga tctcgtatgt gctgtcgccc atgatgcgcc gcctgatggc ctggaaatca
     5161 gggcagagcc ggccgtatgt gccctgggtg ggcctgccca acgtgctgct caatgatttc
     5221 gccgtgccgg aattgctcca ggacgacgcc acgcccgagg cgctcgccaa agccacgcag
     5281 gcggcgctgt acgactcgag cctggcggag cacctgcggc ggcgcttcac cgggttgcat
     5341 gccgatctga cgcgcgatac cgcgggtctg gccgccaagg tcatcctgga acaggcgggc
     5401 gcggcatgag ccaggccggc ctgttcgatc agcctccgcc agtgctgacg gccggtgtcg
     5461 acgaagcggg acgcgggccc ctggccggtg atgttttcgc cgccgcggtc attctggatc
     5521 cggaactgcc gatccccggg ttggctgatt ccaaggtgtt gagcgcgagt cggcgcgagc
     5581 atctggcggg tctcatccgg gagcgggcgc tggcctggca catcgcttcg gcaagcgtcg
     5641 aggaaatcga tgcgctgaac attctccagg ccaccatgtt ggcgatgcgg cgtgcctgcg
     5701 ccgggttgaa tcccgcgccg gaattggcgc tcattgatgg aaaccgtgtg ccccgcccct
     5761 tgccctgcga ggcacgggcc atcgtcaagg gggatgcgct ggtcgcggcg atctcggcgg
     5821 cgtcgattct cgccaagacc gctcgggatg cctactgcgc gtccatgcat caggcgtatc
     5881 cgcagtatgc gttcgatcag cataaaggct atggtacggc gctgcatctg gcgcggcttc
     5941 gtgaacacgg cccgtgcccg acgcaccgcc ggagcttcgg tccggtgaag aggcttctgg
     6001 gcgcggaatc ccagccatga aacgcatcag ctcacgtgag aatccggcgt acaagcgggt
     6061 gcttcgcatc gcggaaggca aggagcgcgg ctgggcggtg atcgagggca ttcatttgtg
     6121 ccgcgcgtgg ctcgaccatg tgggccagcc cgacatggcc ctgttcgatg agggcgggct
     6181 gggtccggat ggcgaacagg ccggcctggc ctcgcggctc gacgcgcatc gatgcctggt
     6241 gtgcgagccg cggtcatggc gtgcggtgtc cggggtcgag catggccagg gtgtgctgtt
     6301 cgtggttagt gtgccggaac cggacattcc actgaccctg gaccggaact gcatctggct
     6361 ggatcgcgtg caggatccgg gcaatctcgg aaccctttta cgcacggccg cggcggccgg
     6421 aatagggcag gcgtacctct ctgaagggtg cgccaacgcg tggtcgcaaa aagtcctgcg
     6481 cagcgcccag ggtgcccatt tcgtcatggc gatccacgag cgccaggaac tgcgggcctt
     6541 gcgggcccgt ctgcgtattc cactggtggc gacatcgttg cgtgacgccc ggtcgattga
     6601 cgacgtcgcg cttccggccg actgcgcctg gttgttcggc aatgaaggac gtggtgtggc
     6661 ggacgatctt ctggacgcgg ccgatgagcg ggtgttcatc ccgcaagtcg cgacggtgga
     6721 gtcgttgaac gtggcggcgg cggcggcgat ctgcctgttt gagcaacgtc ggcggcacag
     6781 ggtcggcgcc gcgtaggccc accctttccg gcgtgcgtgc atcggccggt gaaggcattt
     6841 gg
//
LOCUS       DASTNK010000155         5594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1621235, whole genome shotgun sequence.
ACCESSION   DASTNK010000155 DASTNK010000000
VERSION     DASTNK010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621235"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..181)
                     /locus_tag="VFR20_11730"
     CDS             complement(32..181)
                     /locus_tag="VFR20_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231236.1"
                     /translation="MPGKHDKPSLLAILRRELALLWPVFLVLLGVVVVPALVVVGILK
                     IVGVK"
     gene            complement(254..1183)
                     /locus_tag="VFR20_11735"
     CDS             complement(254..1183)
                     /locus_tag="VFR20_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0231237.1"
                     /translation="MSEPDFNLLMALDALLAEGGVAGAARRVGLSTSAMSRTLGRLRE
                     VTGDPLLVRAGRHMVLTPYAQAIRERARNAVLETQSLLRPAMSELDLPALDRVFALRA
                     NEGFIEVFGPSLIAAVAEVAPNVCLRFTPKLEKDSRYLREGLADLEIGVVHNMGPEIR
                     IQALFRDHFVGVVRAGHPLLAESAVTTERYLSYGHVVASPRGLSRDYADDALADMGLA
                     RKVVSVVPGFPTALSVAMASDLIALLPVSYLPTPATGGQRTAGFQFFELPFPTPIITI
                     SLMWHPRLEMEPANRWLRQLVRRVCREQVASRD"
     gene            1360..2763
                     /locus_tag="VFR20_11740"
     CDS             1360..2763
                     /locus_tag="VFR20_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0231238.1"
                     /translation="MMELFTDKPGDEGLPGTERAAAMAALMTATVMVVFDGTVINIAL
                     PKMASALQVSPAVAVWFTNGYLLSVAMTLAIFAALARRVGFRALFLFGLALFTLASAG
                     CAMMSNAYALIAMRVLQGLGGAAVVSIGPAILRSVFPGRLLGRILGVNALLIAASTAI
                     APIVGGTLLDAWGWEWLFAMNIVPGLIALGLAMKAIPGRPATVREPFDTSGAVLSAVL
                     VGALVMAANHAMLPDGGWYGLVALVAVVGFVWRLRQARAPLLPPAIFTSSRFSLAALT
                     SMASFVGQGITFVALPFLFQNVYGYNAFETALLFTPWPIGIVLVAPHAGRLADKHSPA
                     MISTIGLVVLVAGLVSLALLPAQPQVWEIGVRCFVCGAGFGCFQSPNNREMLSNVSRE
                     NSGYASGVLAIMRTFGQCLGTALVGVLLAVYAQGGATVMESRAVHMCLWVAVAATVMA
                     VGFSLGRLRGTSYRLAS"
     gene            2964..3222
                     /locus_tag="VFR20_11745"
                     /pseudo
     CDS             2964..3222
                     /locus_tag="VFR20_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228770.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            3330..3608
                     /locus_tag="VFR20_11750"
     CDS             3330..3608
                     /locus_tag="VFR20_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011458603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU0231239.1"
                     /translation="MIKTFRHKGLKAFFEQGTVAGIQAVHANRLGAMLRRLNETTGPQ
                     GMNLPGWGLHSLKGDLKGHFSVQVSGNWRMTFMFDDGDAVLVDYQDYH"
     gene            3618..3941
                     /locus_tag="VFR20_11755"
     CDS             3618..3941
                     /locus_tag="VFR20_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEU0231240.1"
                     /translation="MRMHNPPHPGLTLRDDVLPALGLSITDAAKQLGVARVTLSRVLN
                     GHAAISPEMALRIEAWLGRDRGGEARLWLAEQTAYDMWQAEQRIKVTPLHVQRAPAVH
                     DLIAA"
     gene            complement(4240..4315)
                     /locus_tag="VFR20_11760"
     tRNA            complement(4240..4315)
                     /locus_tag="VFR20_11760"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4280..4282),aa:Thr,seq:tgt)
     gene            complement(4388..>5594)
                     /locus_tag="VFR20_11765"
     CDS             complement(4388..>5594)
                     /locus_tag="VFR20_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEU0231241.1"
                     /translation="LGIVRPDGRVATRNYIGILTSVNCSATVARSIADHFRRDINPAA
                     LADYPNVDGVVALPHATGCGMGAKGLFMDTLRRTLIGYMRHANFHSVLMVGLGCETNQ
                     IPAILATGGLHENDRLQTFTIQDTGGTAKSVAKGIEKVKEMLPAANQVTRTPVSASHI
                     VVGLQCGGSDGYSGITANPALGAAVDRLVRAGGAAILSETPEIYGAEHLLTQRAVSRE
                     VGEKLVERIKWWEDYCSRNFAELNNNPSPGNKAGGLTTILEKSLGAVAKAGTTNLNEV
                     YQYAEPVTAKGMVFMDSPGYDPVSATGQVASGANMICFTTGRGSAYGCTPSPSLKLST
                     NNALWERQEEDIDINCGTIISGEETVDSLGEKIYRLMLETASGRKTKSEVHGYGQNEF
                     APWVVGVTV"
BASE COUNT          993 a         1702 c         1829 g         1070 t
ORIGIN      
        1 ccgttcccac gatgcgacag ggtggacgaa atcacttcac cccgacgatc ttgaggatgc
       61 cgacaaccac cagcgcgggc acgacgacaa ccccaagcaa cacgagaaag accggccaca
      121 gcagcgccag ttcccgacgc aggatggcaa gaaggctggg tttgtcgtgt ttgcctggca
      181 cagtcaatcc tcaagcacgg gcacgcaagc cgcgcccaac ccttcaaact gtacccctcg
      241 ggcggggcgc cgtctaatcg cgggaagcga cctgttctcg acagacacgc cgcaccagtt
      301 gccgcaacca gcggttcgcc ggctccattt ccaggcgcgg gtgccacatc agtgaaatcg
      361 tgatgatcgg agtcgggaag gggagttcga agaactggaa ccccgccgtc cgctgcccgc
      421 ctgtggcggg ggtgggcaga tacgacacgg gaagcagtgc gatcaggtcc gatgccatcg
      481 ccacggaaag ggccgtcgga aatcctggca ccacgctgac caccttgcgg gccaggccca
      541 tgtcggccag ggcgtcgtcg gcgtaatcgc gggacagccc gcgaggcgaa gcgacgacat
      601 gtccgtatga caagtatcgc tcggtcgtga cggccgattc ggccagcaat ggatggccgg
      661 ccctgaccac accgacgaaa tgatcgcgga acagcgcctg gatacggatc tccggcccca
      721 tgttgtggac cacgccgatc tccaggtcag ccaacccttc ccgaaggtag cgggagtcct
      781 tttccagttt cggcgtgaag cgcaggcata cgttcggcgc cacctctgcc acggcggcga
      841 tcagcgaggg gccgaacact tcgatgaacc cttcgttggc gcgcagggcg aacacgcgat
      901 ccagcgcggg caggtccagt tcggacatcg ccggccgcag cagggattgc gtttcgagca
      961 ccgcgttgcg cgcgcgctcg cggatggcct gcgcataagg tgtcaacacc atgtggcgtc
     1021 ccgcccgcac caggaggggg tcgccggtga cctcgcgcaa ccgccccagc gtacggctca
     1081 tggccgaggt gctgaggccg acgcggcgcg ccgcgcccgc cacgccaccc tcggcaagca
     1141 gcgcgtcgag cgccatcaac aggttgaaat cgggttcgga cattccagaa ggcgcccata
     1201 taagaatccc cggctgttgc ggaagcgctg ccagggagat tgacggaggg ggcacaaagt
     1261 tgcatgaaac acaactaata agtgcaaatg gtgcgtcttc cgccttgtca tcgccgcgat
     1321 tatatttccc caacgaatga aacggcaatg aggtgaaata tgatggaatt attcacagac
     1381 aagcctggcg atgagggtct gccgggcacg gagcgcgccg ccgccatggc ggcattgatg
     1441 accgccaccg tcatggtggt gttcgacgga acagtgatca acatcgcgtt gccgaagatg
     1501 gccagcgcgt tgcaggtgtc gccggcggtg gcggtgtggt tcaccaacgg ctacctgctg
     1561 tcggtggcca tgaccctggc catcttcgcg gcgctggcca ggcgagtggg gtttcgggcg
     1621 ctgtttcttt ttggccttgc gctgttcacc ctggcgtcgg cgggttgcgc gatgatgtcg
     1681 aacgcttacg cgttgatcgc catgcgggtc ttgcagggcc tgggtggcgc ggcggtggtc
     1741 agcatcgggc cggccatatt gcgatccgtg ttccccggcc ggctgctggg ccggatcctg
     1801 ggtgtgaacg cgttgctgat cgccgccagc acggccatcg cgccgatcgt tggcggcacg
     1861 ctgctcgacg cgtggggctg ggagtggctg ttcgccatga acatcgttcc aggcttgatt
     1921 gcgctgggtc ttgccatgaa ggccattccc ggacgcccgg ccactgtgcg tgaaccgttc
     1981 gacacgtcgg gggcggtgtt gtccgccgtg ctggtggggg cgttggtcat ggcggccaat
     2041 cacgcgatgc tgccggacgg tggctggtat gggctggtcg cgctggtggc cgtcgtgggg
     2101 tttgtgtggc gtctgcgtca ggccagggcg ccgctgcttc ccccggctat tttcacgtca
     2161 agccggttct cgctggccgc gttgacctcg atggcgtcat tcgtggggca gggcatcacc
     2221 ttcgtggcgt tgccgttcct gttccagaac gtgtacggct acaacgcgtt cgaaacggcg
     2281 ctgttgttca cgccgtggcc gatcggcatc gtgctggtgg cgccgcatgc cgggcggctg
     2341 gccgacaagc actcccccgc catgatctcc accatcggct tggtcgtgct ggtggcgggc
     2401 ctggtgtcgc tcgccctgct gcccgcgcag ccgcaagtct gggagatcgg tgtgcgctgc
     2461 ttcgtgtgcg gggcgggctt cggttgtttt caaagcccca acaatcgtga aatgctgtcg
     2521 aacgtgtcgc gggagaacag cggctacgcg tcgggggtgc tggccatcat gcgcacattt
     2581 ggtcagtgcc tggggacggc gctggtgggg gtgttgctgg ccgtgtatgc ccaggggggc
     2641 gccacggtga tggagtcgcg ggcggtgcac atgtgcctgt gggtcgcggt ggccgcgacg
     2701 gtgatggcgg tggggttcag cctgggccgg ctgcggggaa cgtcttatcg actggcctcg
     2761 tgagccctag ggttcctcgc ggttttcact tcgcctgaaa cagcaaaatg ccaacccttc
     2821 ggcttttgta tggttccgct cagccgcgaa gggtggaatg gccttatctt gctattggct
     2881 gggggcgaca agcgaaggca acaggccgat atcgacgccg ccattgccaa ctggaaagac
     2941 tggcaagacc gcgagaaagc atcatgatca cacaccgcac ttccaccagc cgtccgcacg
     3001 agcagacagt catcgacatg ctgcgcgatg acccggaatt cttggatgag taccttgccg
     3061 tggccctgga tgaaatcgac cagcccggcg ggcaggccgc gcgaaagctt gtaccgtgcc
     3121 ttgtcttcca agggcaaccc caccattaaa accctgctgg ccgtcatcaa tggtgttggc
     3181 ctgcatttgg ccgttacccg tgccaaccac accccggcat gacctcttac gcggcgccta
     3241 gggtagctcc cgaaaagccg gaaaccttca tcgccaagcc gaaggcagta gttgtttgat
     3301 agctgatacg ttacgcgata caatggtcca tgataaagac attccggcac aaaggactga
     3361 aagcgttttt cgagcaagga accgttgcgg gtattcaggc cgtacatgca aacaggctag
     3421 gggccatgct gcgacgcctg aacgaaacga ctggcccgca aggcatgaac ttgccgggtt
     3481 ggggcctgca ttcgttaaaa ggcgacctca aagggcattt ttcagtgcag gttagcggca
     3541 actggcgcat gacctttatg tttgatgatg gcgacgccgt gctggtcgat tatcaggatt
     3601 atcactagga gaacaccatg cgtatgcata accccccgca tccggggctg accttacgcg
     3661 atgacgtatt gccagcgttg gggctttcca tcacggatgc cgcaaaacag ttgggcgtgg
     3721 cccgcgtcac gctatcccgc gtcctgaacg gccatgcggc catatcgcca gaaatggcgc
     3781 tgcgcattga ggcatggctg gggcgtgacc gtggcggcga ggcgcgcttg tggttggcag
     3841 aacaaaccgc ttatgacatg tggcaggctg aacagagaat caaggttacg ccgttgcacg
     3901 tccaacgagc gccagcagtg catgacttaa tcgccgcctg acccttttac gccagcatct
     3961 agcccgacgg ggcgaaaagc cggaaacctt caccggcctg gtgctggcac cctcattgaa
     4021 gtttgcacag tgaaggcgtg cgcatgaatt tcatttcctt gggcgaactg gtcgagatcg
     4081 cggtggggct tcaaaagtgg aaatcaacga agccgccaca atccgcgccg aggcgcgtac
     4141 gtccacatag cggctacata gagccagaag ccaaaacgcc aaccccgaga ggctggccta
     4201 agtgcttgag taagtgcttg agtaagtgct tgattcaact ggtgccggtt agaggagtcg
     4261 aacccccgac cttcgcatta caagtgcgct gctctaccaa ctgagctaaa ccggcaagcc
     4321 cgttattcta ctggaatttg accggtggcg cggcgtttcg cactggcctg gacatgggcg
     4381 cccgccccta caccgtcacc cccaccaccc acggcgcgaa ttcgttctgg ccatacccgt
     4441 gcacttcgct cttggtcttg cgccccgacg cggtttccag catcagccgg taaatcttct
     4501 ctcccaggct gtccacggtt tcctcgccgc tgatgatcgt gccgcagttg atgtcgatgt
     4561 cttcttcctg gcgctcccac agcgcgttgt tggtgctgag cttgagcgac ggcgacggtg
     4621 tgcaaccgta ggccgagccg cgtccggtgg tgaaacagat catgttggcg ccggatgcca
     4681 cctggccggt ggccgacacc gggtcgtagc ccggggaatc catgaacacc atgcccttgg
     4741 ccgtgacggg ctcggcgtac tggtagacct cgttcaggtt ggtcgtgccg gccttggcca
     4801 cggcgcccag ggatttttcc agaatggtgg tcaggccgcc cgctttgttg ccgggcgagg
     4861 ggttgttgtt cagttcggcg aagttgcgcg aacaatagtc ttcccaccac ttgatgcgct
     4921 cgaccagttt ttcgcccacc tcgcggctga ccgcgcgctg cgtcagcaag tgttcggcac
     4981 catagatttc cggggtttcc gagaggatgg ccgcgccgcc ggcgcgcacc aggcgatcga
     5041 ccgccgcgcc cagtgcgggg ttggcggtga tgccggaata accatccgag ccgccgcact
     5101 gcaggcccac gacgatgtgc gaggccgaca ccggcgtgcg cgtgacctgg ttggccgcgg
     5161 gcagcatttc cttcactttc tcgatgccct tggccaccga cttggcggtg ccgcccgtgt
     5221 cctggatggt gaacgtctgc aggcggtcgt tctcgtgcag gccgccggtg gccaggatgg
     5281 ccggaatctg gttggtttcg cagcccagcc ccaccatcag cacggaatgg aagttcgcgt
     5341 ggcgcatgta gccgatcagc gtgcgccgca gcgtgtccat gaacaggccc ttggcgccca
     5401 tgccgcagcc cgtggcgtgc ggcagcgcca cgacgccgtc gacgttcggg tagtcggcca
     5461 gggcggccgg gttgatgtcg cggcggaagt ggtcggcgat ggatcgcgcc accgtggccg
     5521 agcagttcac cgaggtgagg atgccgatgt agttgcgcgt ggcgacccgg ccgtccgggc
     5581 gcacgatgcc cagg
//
LOCUS       DASTNK010000156        15434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_881687, whole genome shotgun sequence.
ACCESSION   DASTNK010000156 DASTNK010000000
VERSION     DASTNK010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15434
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881687"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2815
                     /gene="infB"
                     /locus_tag="VFR20_11770"
     CDS             <1..2815
                     /gene="infB"
                     /locus_tag="VFR20_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU0231242.1"
                     /translation="ELSSVDDAVTDADKAKLLDSLRRSHGGDEGKKITLTRRQTSEIR
                     QADSSGRSRTIQVEVRKKRVFVKRDPAEIVAEAKAASEDTSAELAVVSEAVETPDHAI
                     APVAPETASPVLPDEVHDEPVAAIVPEPAPEPEAIEAVVPNEPDAPSAPEAAPAAPEV
                     APVAASAQEAATAQPVAESANEGDEGDTPVSAKPADKAPRNTAKARRAAAVEANPNHE
                     ADEERERARRASEAEAAALRAMLNRPRKVLTAPEPAGLSGTLHKPAAVKGAVKKDAKA
                     GTEPKKVIKTADVSSSWSDDSGRKKPAARADAAPSTPSRDGWRAGGKAGRGGGRAGRG
                     ARGRNAQAEHKDMQPTEFIAREVHVPETITVADLAHKMSVKAAEVIKQLMKLGQMVTI
                     NQVLDQETAMIVVEELGHQAIAAKLDDPEAFLDVDDDTQSEAEALPRAPVVTVMGHVD
                     HGKTSLLDYVRRTKVASGEAGGITQHIGAYNVQTSKGMITFLDTPGHEAFTAMRARGT
                     KATDIVILVVAADDGVMPQTKEAIHHAKAAGVPLVVAINKIDKQDANPDRVKQELVAE
                     EVVPEEYGGDVPFVSVSAKTGQGIDDLLDNVLLQAEMLELKAPVEAPAKGIVIEARLD
                     KGRGPVATILVQSGTLSRGDAVLIGASYGRVRAMLNENGKPINSAGPSIPVEIQGLTE
                     VPAAGDEVIVLADERKAREIALFRQGKFRDVKLARQQAAKLESMFDNIGEGSQTLALI
                     VKTDVQGSQEALVTALVKLSTNEVRVDVVHAAVGGISESDVNLAIASHAVVIGFNVRA
                     EQSAKKLAEHNGIDLRYYNIIYDAVDDVRNAMSGMLAPEKKEEVIGLVEIREVFNVSR
                     VGNIAGCMVVDGLVRRDSQARLLRNNVVQWTGMLDSLRRFKDDVKEVKSGFDCGITLK
                     GNNDIQIGDQLEIFEIKEVARTL"
     gene            2818..3222
                     /gene="rbfA"
                     /locus_tag="VFR20_11775"
     CDS             2818..3222
                     /gene="rbfA"
                     /locus_tag="VFR20_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU0231243.1"
                     /translation="MGRHKYKPNPGRNTRLADQIQKDLAGLIQREIDIARAGLVTLTG
                     VDLSADYAHAKVYFTVMGAEPQAAAEALNEKAGWLHSLLFKMLHIHTVPTLRFVHDDQ
                     LARGIALSHLIDQANRPGVFTPAVPDDPDEQP"
     gene            3248..4006
                     /gene="truB"
                     /locus_tag="VFR20_11780"
     CDS             3248..4006
                     /gene="truB"
                     /locus_tag="VFR20_11780"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU0231244.1"
                     /translation="MATRRGQALDGVLLLDKPEGLSSNHALQRARRALDARKAGHTGT
                     LDPFATGLLVCCLGRATKIAGIMLDADKAYEATLRFGEETDSGDLTGHVQFSAPTPFG
                     GVSREELETAMEGFRGEIEQIPPMHSALKRDGKPLYEYARQGITLERASRRVVVHRLE
                     TLDFSGTEARLLVHCSKGTYVRTLAQDIGRRLGCGAHLVALRRTRVGPFRIEDAVALD
                     ALQSMANPASRLTPLDALPDGLLPDTLKQKDTSS"
     gene            4003..5823
                     /gene="typA"
                     /locus_tag="VFR20_11785"
     CDS             4003..5823
                     /gene="typA"
                     /locus_tag="VFR20_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU0231245.1"
                     /translation="MNRALRNVAIIAHVDHGKTTLVDQLLRQSGTFRDNQQISERVMD
                     SGDIERERGITILSKNCAVEYEGTHINIVDTPGHADFGGEVERVLSMVDGVLLLVDAV
                     EGPMPQTRFVTRKALALGLRPIVVVNKIDRPGARPDHVVNATFDLFDKLGATEEQLDF
                     PVVYASGLSGYAGLTDDVREGDMRPLFEAILKHVPQREDDAEGPLQLQIVSLDYSSYV
                     GKIGIGRINRGRVRAGQDVLVKFGPEGESIKGRINQVLTFHGLERKLVDEAQAGDIVL
                     INGIEELGIGCTVMDPEQAEALPLLRIDEPTLTMNFMVNSSPLAGREGKYVTSRQVRD
                     RLERELKSNVALRVNDTADDMVFEVCGRGELHLTILLENMRREGYELAVSRPRVVLKE
                     IDGVRMEPFELLTVDVEDGHQGSVMEELGRRKGDLQDMVADGRGRTRLEYRIPARGLI
                     GFQSEFLTLTRGTGLMSHIFDDYAPMREGGVGERRNGVLISQDDGAAVAYALWKLQER
                     GRMFVSPGEALYEGMIIGIHSRDNDLVVNPIKEKKLTNVRSSGKDEHIDLVPPVRLTL
                     EYAVEFISDDELVEVTPKSIRLRKRYLQEHERRRAQREGA"
     gene            6002..6784
                     /locus_tag="VFR20_11790"
     CDS             6002..6784
                     /locus_tag="VFR20_11790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0231246.1"
                     /translation="MSVDAAGKGVQGKVALVTGAGSGIGRAVALELLAHGYRVVLAGR
                     RGETLEQTIGLASTEAGTRALAVPTDVSDEQAVDALFAEIQRVHGRLDVLFNNAGRGA
                     PAVPIDELPVETWRAVVDVNLTGMFLCARAAVRLMKVQQPRGGRIINNGSISAHAPRP
                     MTVAYTATKHAVTGLTKSLSLDGRAHDIVCGQIDIGNAATDMTERMAAGILQADGGIR
                     VEPRMDVREVARAVRHMADLPLGVNTQFMTIMASAMPYVGRG"
     gene            complement(6793..8271)
                     /locus_tag="VFR20_11795"
     CDS             complement(6793..8271)
                     /locus_tag="VFR20_11795"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEU0231247.1"
                     /translation="MTSQTLPITLKDSSLFKTQSYIDGKWTAGENGATFGVDNPVDGA
                     IIAQVANLDATHAQAAIDAADKALPAWRARTGKDRAKLLRNWFDLILANAEDLARIMT
                     LEQGKPIAESRGEITYGASFVEWFAEQAKRVNGDVLASPGPNRMLVLRQAIGVCAAIT
                     PWNFPNAMITRKVAPALAAGCTVVLKPAEQTPISALALAELAHRAGIPAGVLNIISAD
                     SENSVAIGKTLCASPVVKKVTFTGSTSVGRLLMQQSAPTIKKLSFELGGHAPFIVFDD
                     ADIDAAVEGAMISKYRNTGQTCVCTNRFYVQAGAYDEFVKKFAAKVDALKVGNGAEEG
                     NSQGPLIDDAAVAKVREHVEDAVKKGGNVVVGGKPHALGGRFFQPTVVTSVTEDMLCM
                     HEETFGPVAPVVKFTDEQHVVELANNTEYGLASYFYSRDIGRIFRVAEALEYGMVGVN
                     TGLISNEVAPFGGVKQSGLGREGSIYGMDDFLEMKYVCLGGI"
     gene            complement(8383..9051)
                     /locus_tag="VFR20_11800"
     CDS             complement(8383..9051)
                     /locus_tag="VFR20_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEU0231248.1"
                     /translation="MTINKMVQVATTGQQTEITTELQQAADQFLEIGYGVSVFGSARI
                     QPDSPYYAMAQELGRRLAKAGITVIAGGGPGIMEAANRGAFEAGGKSVGLNILLPRET
                     GDNPYQTHSLHFKHFSARKTTFFAHSIGYIILPGGFGTLDELFEILTLEQTRKTAPVP
                     IVMVGAEYWSGLIDWIKTQTLALGLISPHDLDLFKISDNVDAIMRVIEESCGNYVEST
                     DCAI"
     gene            9050..11779
                     /gene="polA"
                     /locus_tag="VFR20_11805"
     CDS             9050..11779
                     /gene="polA"
                     /locus_tag="VFR20_11805"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU0231249.1"
                     /translation="MKKTLLLVDGSSYLYRAYHAMPDLRNARGEPTGALYGVLSMLRR
                     LAQDYKADYAACVFDARGGTFRNDLYPEYKANRPSMPEDLAAQIEPIHQAVSALGWPV
                     LAVSGVEADDVIGTLARRATHEGVRTVISTGDKDLAQLVNEDVELVNTMSGERQDVAG
                     VVRKFGVAPEQIIDFLMLTGDTVDNVPGVEKVGPKTAAKWLTAYQSVDALVARADEIK
                     GTAGQNLRKAIPGFDLTRRLLTIKTDCELPMLSDGLGGLELRPADDQTLIGLYDQYGF
                     RTWLRELSGDPDRVPAQDARVAPEARDKAETPADTHYDTILDWAAFDQWLERIKMANL
                     VAFDTETTSLDPMLARLVGLSFATAPGEACYIPVGHRGPDRTEQLPRAEVIARLTPWL
                     SDPRAAKLLHNSKYDAHVLRNEGIALAGIAEDTMLQAYVLESHRRVNLQELGERWLGR
                     TGVTYEDLCGKGAKQIGFDEVDIEKAAYYACEDADFTLRLHHALRPRVAADAGLEKIY
                     LLEIATSAVLTTIERNGVRIDAHVLAMQSHELGEQLLALEKKAYELAEQPFNLNSPKQ
                     LGEILFERMRLPVVRKTASGAPSTDEDVLTRLAHDYPLPQVLLEYRGVAKLKSTYTDK
                     LPRMINPATGRVHTRYAQAAVITGRVASSDPNLQNIPVRTAEGRKVRTAFVASGGGQI
                     VSADYSQIELRVMAHVSDDENLQNAFRHGEDIHRATASEVFGVALGDVSSEQRRAAKA
                     INFGLIYGMSEFGLASSLGITRDAARAYMDRYFARYPRVAEYMDRTRHKARENGYVET
                     VFGRRLWLPDIRGAKGPRLAAAERAAINAPMQGTAADLIKMAMVAVQEWIAEQKLATL
                     MVMQVHDELVLDCPAEEVETVVQRVPALMCQVAELRVPLVAEVGVGANWEQAH"
     gene            11785..12579
                     /locus_tag="VFR20_11810"
     CDS             11785..12579
                     /locus_tag="VFR20_11810"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU0231250.1"
                     /translation="MTHSEGSIMSSTSTIHTSSAGLEQGLFDLPAPGQSIKAYFARPA
                     GTGAFPVILVIQEIFGLNDHIQDVCRRFAHQGYLAVAAELTQRQGDAGSYSDIPTLVR
                     DVVANVPDEQAMADLDACAAWAMAHGGAAGQLGATGFCWGGRIAWLYAAHNPDCKAAV
                     AWYGRIAAGHGPLQTHQPIHLANELHAPVLGLYGGKDTGIPVDDVRALEVLLAQGNDA
                     ARASRFKVYPESGHAFFADYRPSYRAEDARDGWRLALDWFEGHVRK"
     gene            complement(12620..14281)
                     /locus_tag="VFR20_11815"
     CDS             complement(12620..14281)
                     /locus_tag="VFR20_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEU0231251.1"
                     /translation="MSEILALLDFAGYVALLLWGVHMVQSGVQRAFGAALGTALSHAL
                     GTRLRAFLAGLGITAALQSSTATGLMITGFAAGGLVALGPGLAAMLGANVGTTLIVQV
                     LSFNLTALAPALILVGVWLFRSNQPGRRRDLGRVFIGLGLLLLSLHELVTLFAPIQHA
                     TLFHTILDALATQPFLALLLATVLTWAAHSSVAVVVLIMSLSEHGLVAPGVAYALVLG
                     ANLGTAINPILEGVNDNDPAARRLPMGNLGTRVVGCVLAMIVLPWLPAAMSALTSSPA
                     RAVANFHTLFNVVIAVAFMPFLKPYAKLLTRLMPKRVDPNDPSKPMYLDEAAHEVPAV
                     ALGNAAREALRLSDMLQAMLNIARAGYLRDNRHRVAQAHTINNGLTRLDNHITAYLAT
                     LDQENMNQDDHRRLEEVLTFSANITHAASITSNGLLGHTSTLRKKGWSLTGEQRQELT
                     HVLDRLIRNQRQTAALFVAEDLKSARQLAFEKDEFRAIESAAAERQLQKIKSGQIESA
                     EIGSLYLEILRDVKTINSYLVGAAAYPILARHNELLPNRLRATEN"
     gene            complement(14309..15187)
                     /gene="htpX"
                     /locus_tag="VFR20_11820"
     CDS             complement(14309..15187)
                     /gene="htpX"
                     /locus_tag="VFR20_11820"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEU0231252.1"
                     /translation="MKRIVLFLLTNFAVMLVLGVVMSIFGVGRFLTANGLNLSQLLVF
                     CAIVGFTGAIFSLLISKWMAKQSTGARVIDPHAPANATEAWLVDTVHQLADRAGVGHP
                     EVAIYDGAPNAFATGAFKNDALVAVSTGLLESMSEEEITAVLGHEMSHVANGDMVTLT
                     LIQGVVNTFVLFLSRIVGYFVDRVLLKNERGVGPGYYVSVIVCEIVFGILASIIVAWF
                     SRLREYRADAGSARLLGSRDPMIHALARLGGMESGDLPKTFEASGISAGGAASLFASH
                     PPIAARIQALRNLNPA"
     gene            15322..>15434
                     /locus_tag="VFR20_11825"
     CDS             15322..>15434
                     /locus_tag="VFR20_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HEU0231253.1"
                     /translation="MQSLWMLLACAMFAAMGACVKVAFDYGASLPEIVLFRG"
BASE COUNT         2892 a         4603 c         5103 g         2836 t
ORIGIN      
        1 tgagctcagt tcggtcgacg acgccgtcac tgacgcggac aaggcgaaac tgctcgattc
       61 gctgcgccgt tcccacggtg gagacgaggg caagaaaatt accttgacgc gccggcagac
      121 ctccgagatc cgacaggccg atagttcggg ccgatcccgg accatccagg tcgaagttcg
      181 caaaaaacgc gtgttcgtga aacgggatcc cgccgagatc gtggccgagg ccaaggcggc
      241 gtcggaggac acgtcggcgg agttggcggt cgtatccgaa gcggttgaaa ctccggatca
      301 cgccatcgcc cctgtcgcac ccgagacggc cagcccggtt ctcccggacg aggtgcatga
      361 cgagcccgtc gcggccatcg ttccggagcc cgcgcccgaa cctgaagcga ttgaagcggt
      421 ggttccgaac gaacccgatg cgccgtcggc gccggaagcc gccccggccg cccctgaagt
      481 agcgccagtc gccgcgtcgg cacaggaagc cgcaaccgcg cagcccgtgg ccgagtcggc
      541 caacgagggc gacgagggcg acacccccgt ttcagcgaaa ccggccgaca aggcgccacg
      601 caacacagcc aaagcgcgcc gcgccgccgc ggtcgaagcg aatccgaatc acgaggccga
      661 cgaagagcgt gaacgcgccc gtcgcgcttc ggaagcggaa gcggccgcct tgcgcgccat
      721 gctgaatcgg ccccggaaag tgttgacggc tccggaaccc gcgggtctgt cgggcacgct
      781 gcacaaaccc gcagcggtca agggggcggt caagaaggac gccaaggcgg gaaccgagcc
      841 taagaaggtg atcaagaccg cggacgtttc ctcgtcctgg tccgacgaca gcggcaggaa
      901 gaaaccagct gcgcgcgcgg atgcggcccc gtccactccc agccgtgatg gttggcgtgc
      961 gggcggcaag gcggggcgcg gcggtggtcg cgccggacgt ggcgcgcggg gacgcaacgc
     1021 acaagccgaa cacaaggata tgcagcccac cgagttcatc gcgcgcgagg tgcatgtgcc
     1081 cgaaaccatc accgttgccg atctcgccca caaaatgtcg gtgaaggccg cggaggtcat
     1141 caagcaactc atgaagcttg gccagatggt caccatcaat caggtgctcg accaggaaac
     1201 cgccatgatt gtggtcgagg aactggggca ccaggccatt gccgccaagc tcgatgatcc
     1261 ggaagctttc ctcgatgtcg acgacgatac gcagtccgag gccgaggcgc tgccccgcgc
     1321 accggtggtc accgtcatgg gccacgtcga tcacggcaag acgtcgttgc tcgattacgt
     1381 gcgccgcacc aaggtggcgt cgggcgaggc cggtggtatc acgcagcata tcggcgcata
     1441 caacgtccag accagcaaag gcatgatcac cttcctcgat accccggggc acgaggcgtt
     1501 cacggccatg cgtgcgcgtg gcaccaaggc gaccgacatc gtcatcctgg tggtggccgc
     1561 cgatgacggc gtgatgcccc agaccaaaga ggccattcac catgccaagg cggcgggtgt
     1621 gccgctggtg gtagccatca acaagatcga caaacaggat gccaaccccg accgcgtcaa
     1681 acaggagctg gtcgccgagg aggtcgttcc cgaggaatac ggtggcgacg tgccgttcgt
     1741 gtctgtgtcg gccaagactg gtcagggcat cgatgatctg ctcgacaacg tcctgttgca
     1801 ggctgagatg ctcgaactca aggcgccggt cgaagcacct gccaagggta tcgtgatcga
     1861 agcccggctg gacaaggggc ggggccccgt ggccacgatc ttggtccaga gcggtacctt
     1921 gtcgcgtggc gacgcggtct tgatcggcgc cagctatggc cgggtgcgtg ccatgctcaa
     1981 cgagaatggc aaacccatca acagcgcggg tccttccatt cccgtcgaaa tccagggcct
     2041 caccgaggtt ccggccgcgg gcgatgaggt catcgtgctg gccgatgagc gcaaggcgcg
     2101 cgaaatcgcg ctcttccgcc agggtaagtt ccgcgacgtc aagctggccc ggcagcaggc
     2161 cgccaaactc gaatccatgt tcgacaatat tggcgaaggc tcgcaaacac tggccctcat
     2221 cgtcaagacc gacgttcagg gctcccagga ggcgttggtt accgccctgg tcaagctgtc
     2281 caccaatgag gtccgtgtgg atgtggtcca cgccgctgtg gggggtatct ccgaaagtga
     2341 cgtgaatctg gccatcgcct cgcatgcggt ggtcatcggt ttcaacgtgc gcgccgagca
     2401 aagcgccaag aaactggccg agcacaacgg aatcgatctg cgctactaca acatcatcta
     2461 cgatgccgtg gacgacgtgc gcaatgccat gtcgggcatg ttggcgcctg agaagaaaga
     2521 agaagtcatt ggcttggtcg agatccgcga agtcttcaac gtctcgcgcg tggggaacat
     2581 cgcgggctgc atggtggtcg acgggctcgt gcgccgggat tcccaggcgc ggctgctgcg
     2641 gaacaacgtg gtgcagtgga ccggcatgct cgattccctg cgccgcttca aagacgatgt
     2701 caaggaagtc aagtccggct tcgactgcgg tatcacgctc aagggcaaca acgatatcca
     2761 gatcggcgac caactcgaaa tcttcgagat caaggaagtc gccaggacgc tgtgatcatg
     2821 ggtcgacaca aatacaagcc aaaccccggc cgcaacacgc ggctcgccga ccagatccaa
     2881 aaggatctgg ccggcctgat tcagcgtgag atcgatatcg cgcgcgcggg gctggtcacg
     2941 ctcacgggcg tggatctgtc ggctgattac gcgcacgcca aggtgtattt cacggtcatg
     3001 ggggcagaac cccaggccgc agccgaggcg ttgaacgaaa aagcgggctg gctgcattcg
     3061 ctgttgttca agatgctgca catccacacg gtgcccaccc tgcggttcgt gcacgacgat
     3121 caattggcgc gaggcatcgc gctgtcccac ctgatcgacc aggccaatcg gcccggggtg
     3181 ttcaccccgg ccgtgcccga cgatcccgac gaacagccct gaggctgtcg tttcccaagg
     3241 tcaaacgatg gctacgcgac gcgggcaggc gctcgatggt gtcttgttgc tggacaaacc
     3301 agaaggtttg tccagcaatc acgctttgca gcgcgcgcgg cgcgcgcttg acgcgcgcaa
     3361 ggccgggcac acgggcacgc tcgatccatt cgccacgggg cttctcgtct gctgccttgg
     3421 gcgggccacc aagatcgctg gcatcatgct ggatgcggac aaagcctacg aagcgacgtt
     3481 gcgctttggc gaggaaaccg actccggcga tctgacgggg catgtccagt tcagtgctcc
     3541 cactcccttc ggtggggtat ccagagagga gctcgaaacg gcgatggaag ggttccgcgg
     3601 cgagatcgag caaattccac ccatgcattc ggcgctcaag cgtgacggca aacctttgta
     3661 cgagtatgct cgacaaggca tcacgctcga acgcgcatcg cggcgggttg tcgtgcatcg
     3721 cctcgaaaca ctggattttt ccggcaccga ggctcgtctc ctggttcatt gcagcaaggg
     3781 cacctatgtg cgcacgcttg cccaggacat cggtcgccgg ctgggctgcg gcgcacatct
     3841 cgtggcgttg cgccgcaccc gtgtggggcc gttccggatc gaagacgcgg ttgccctcga
     3901 tgccctgcag tccatggcca accccgcctc caggctgacg ccgctggacg cgctgcctga
     3961 cggcctacta cccgataccc tcaaacaaaa ggacacatca tcatgaaccg cgccctgcgc
     4021 aacgtagcca tcatcgcgca cgtcgaccac ggcaagacca cactggtcga tcagttgctg
     4081 cgccagtccg gcaccttccg ggacaatcag cagatatccg agcgggtcat ggactcgggc
     4141 gacatcgagc gagagcgtgg catcacgatt ctctcgaaga attgcgccgt cgaatacgag
     4201 ggcacgcata tcaatattgt ggatacgccg gggcacgccg acttcggcgg cgaggttgaa
     4261 cgggtgctgt ccatggtcga cggcgtgctg cttctggttg atgcggtcga agggcccatg
     4321 ccgcagacgc gtttcgtcac gcgcaaggcg ctggcgctcg ggctgcgtcc tatcgtcgtg
     4381 gtcaacaaga tcgatcggcc gggtgcgcgc cccgaccatg tcgtcaacgc caccttcgat
     4441 ctcttcgaca agctgggcgc gacggaagag caactcgatt tcccggtggt gtacgcctcg
     4501 ggcctgtccg gctacgccgg cctgaccgat gacgtgcgcg agggcgacat gcgccccctg
     4561 ttcgaggcca ttctcaagca tgttcctcag cgcgaggatg atgccgaggg gcctctgcag
     4621 ctgcagatcg tttcactgga ctacagcagc tacgtgggca agatcggcat tggccgcatc
     4681 aaccggggcc gtgtgcgcgc cggccaggat gtgctggtga agttcggccc ggaaggcgaa
     4741 tcgatcaaag gtcggatcaa ccaggttctg acgttccacg ggctcgagcg caagcttgtg
     4801 gatgaggcgc aggccggcga tatcgtgctg atcaatggca tcgaggagct tggcatcggc
     4861 tgtaccgtca tggatcctga gcaggccgag gctttgccgc tgttgcgcat cgacgaaccg
     4921 acgttgacga tgaacttcat ggtgaattcg tcgccactgg ctgggcgtga aggcaagtac
     4981 gtcacgagcc gtcaggtgcg tgaccggctc gagcgcgagc tcaaatccaa cgtggcgcta
     5041 cgcgtcaatg acaccgccga cgacatggtt ttcgaagtgt gtggacgggg cgaactgcac
     5101 ttgacgatcc tgctggagaa catgcggcgc gaaggctatg aattggccgt ctcacggccc
     5161 cgggtcgttc tcaaggaaat cgatggggtg cgcatggaac ccttcgagtt gctgacggtc
     5221 gatgtcgagg acgggcatca aggttccgtc atggaggaac tgggtcgccg caagggtgat
     5281 ctgcaggaca tggtggcgga tggtcgcggg cgcacacgcc tcgaatatcg cattcccgcg
     5341 cgtggcctga tcggtttcca gagcgaattc ctgacactga cgcgtggcac gggtctcatg
     5401 agccatatct tcgacgatta cgcaccgatg cgcgaaggcg gggtgggtga gcggcgcaat
     5461 ggcgtgctga tcagtcagga cgacggggcg gcggtggcct atgcgctgtg gaagttgcaa
     5521 gagcgtgggc gcatgttcgt gagcccggga gaggcgttgt acgaaggcat gatcatcggt
     5581 atccacagcc gggacaatga tctggtggtc aatcccatca aggagaagaa gctcaccaac
     5641 gttcgttctt ctggaaagga cgagcacatc gatctggttc cgcccgttcg cctgacgctg
     5701 gagtacgcgg tggaattcat ttccgatgac gagctggtcg aagtcacgcc caaatccatc
     5761 cggttgcgca agcgctatct acaagagcac gagcgccgtc gcgcgcagcg cgaaggggcc
     5821 tgacgcttcc ccgtctcgag acggcgggtt ccccgaccgt tcatgccttt tgaaatgaac
     5881 aatgccccgg caccggactg atcggcccgg ggcattgttt ctttgggcgc tatctgctga
     5941 aatacaggct ggataggggc cggcgcacgg tcggcccccc acgatggacc ggacggtgaa
     6001 aatgagcgtg gatgcggcgg gcaagggtgt gcagggcaag gtggcgttgg tgactggcgc
     6061 gggcagcggg attggtcgag cggtggcgct tgaactgctg gcgcatggct atcgggtggt
     6121 gttggccggc cgacgcgggg aaaccctgga gcagaccatc gggttggcct cgaccgaggc
     6181 tggaacgcgg gcgcttgcgg tgcccaccga tgtcagtgat gagcaagcgg tcgacgcgct
     6241 gttcgcggaa attcaacgcg ttcacgggcg gttggatgtg ctgttcaaca acgctggccg
     6301 gggtgctccc gccgtaccta tagatgaact tcctgtcgaa acctggcgcg cggtggtcga
     6361 tgtgaatctc accggcatgt tcctgtgtgc gcgggccgcg gtgcgcctga tgaaggttca
     6421 gcaaccacgg ggcggtcgca tcatcaacaa cggatccatt tcggcgcacg cgccacgacc
     6481 catgaccgtc gcctataccg ccaccaagca tgccgtcacg ggcttgacca aatccctatc
     6541 actcgacggg cgggcccatg acatcgtttg cggccagatc gacatcggca acgcagccac
     6601 cgacatgaca gaacgcatgg cggcgggcat tctgcaggca gatggcggca tccgtgtcga
     6661 gccgcgcatg gacgtgcgcg aagtggcccg cgccgtgcgc cacatggccg atctgccatt
     6721 gggcgtcaac acgcagttca tgaccatcat ggccagcgcc atgccgtacg tggggcgggg
     6781 ctgacgcgcc ggtcaaatgc cgccgaggca gacatacttc atttccagga agtcgtccat
     6841 tccgtagatg gagccctcac gcccgaggcc tgactgcttg actccgccaa atggggcgac
     6901 ttcgttggag atgaggccgg tgttgacccc caccatgccg tattccaggg cttcggcgac
     6961 ccggaagatg cggccgatgt cgcggctgta gaagtacgag gccaggccgt attcggtatt
     7021 gttggcgagt tcgactacgt gttgttcgtc agtgaacttg acgaccgggg cgaccgggcc
     7081 gaacgtttct tcgtgcatgc acagcatgtc ttcagtgaca ctggtgacga cggtgggctg
     7141 gaagaagcgg ccgcccaggg catgcggctt gccgccgacg acgacattgc cgcctttctt
     7201 cacggcgtct tcgacgtgct cccgtacttt cgccaccgcc gcatcgtcga tgagcgggcc
     7261 ttggctgttg ccttcctccg cgccattgcc cactttcagg gcatcgacct tggcggcgaa
     7321 tttcttgacg aattcgtcgt aggctcctgc ctgcacgtag aaccggttgg tgcagacgca
     7381 ggtttgtccg gtgttgcggt atttcgagat catggctcct tcgacggcgg catcgatgtc
     7441 ggcgtcgtcg aatacgatga atggggcatg cccgccaagt tcgaaagaga gcttcttgat
     7501 cgtgggggcg gattgctgca tgagcaggcg gccgaccgaa gtggaaccgg tgaacgtgac
     7561 tttcttgacg accggactgg cacagagcgt tttgccgatg gctacggagt tttcactgtc
     7621 ggccgagatg atgttcagca cccccgccgg aatgccggcg cggtgcgcca gttcggccag
     7681 tgcgagagcc gaaatagggg tttgttccgc cggcttgagc acgacggtac agcccgcggc
     7741 cagtgccggc gccactttgc gagtgatcat ggcattgggg aagttccacg gcgtgatcgc
     7801 ggcgcacacc ccgatggcct ggcgcaagac cagcatgcgg ttggggccgg gcgaggccag
     7861 aacgtcgccg ttgacccgtt tggcctgctc ggcgaaccat tcgacgaatg aagcgccgta
     7921 ggtgatttcg ccacgcgact cggcaatggg cttgccttgt tccagggtca tgatccgcgc
     7981 gaggtcttcg gcattggcga ggatgaggtc gaaccagttg cgcagcaatt ttgcgcggtc
     8041 ttttccggtg cgagcccgcc atgcgggcaa tgccttgtcg gcggcgtcga tggccgcctg
     8101 ggcgtgcgtc gcgtccaggt tggccacctg ggcgatgatg gcgccgtcaa cggggttgtc
     8161 gacaccgaac gtggcgccgt tctcgccagc cgtccacttg ccgtcgatgt acgactgtgt
     8221 cttgaatagc gatgaatcct tcagagtgat ggggagggtc tgggaagtca tgttcaaagc
     8281 ctgatgtgag tgatggaacg tgaatgaggg aatggtgtcc ccgagcctgt ccggcggctt
     8341 cgggcggcct cggggactga tgggaaggtg agggcgcccg ggtcagatgg cacagtcggt
     8401 gctctcgacg tagttgccgc aggattcttc aatgacccgc atgatggcgt cgacgttgtc
     8461 ggagatcttg aagaggtcca gatcgtgggg gctgatcagg cccagcgcca gtgtctgggt
     8521 cttgatccag tcgatcaggc cggaccagta ctcggcgccc accatgacga tgggcacggg
     8581 cgcggtcttg cgggtctgct ccagggtgag gatctcgaaa agctcatcca gtgtgccgaa
     8641 gccaccgggt aatatgatgt agccaatgct gtgcgcgaaa aaggtggtct tccgagcgga
     8701 aaagtgtttg aaatgcaggc tgtgggtctg gtaggggttg tcgccggttt cgcgtggcag
     8761 caggatattc aacccgacac ttttgccgcc cgcttcgaat gcgccgcggt tggcggcctc
     8821 catgatgccg gggcctccgc ccgcgatgac cgtgatgcca gccttggcga gacggcggcc
     8881 aagttcttgc gccatggcat agtaagggga gtcgggttgg atgcgggcgc tgccgaaaac
     8941 gctgacaccg tacccgattt ccagaaattg gtcggcagcc tgttgcagct ctgtggtaat
     9001 ttcggtttgc tgcccggttg tcgccacttg aaccatttta tttattgtca tgaaaaaaac
     9061 cttgttgctt gtcgatgggt cgagttattt atacagggcc taccacgcca tgcccgacct
     9121 caggaacgcc cggggtgagc ccacaggcgc gctgtacggt gtcctgtcca tgttacggag
     9181 actagcacag gattacaagg cggattacgc cgcgtgtgtg ttcgatgccc ggggcgggac
     9241 gttccgcaac gatctttacc cggaatacaa ggccaaccgg ccctctatgc ccgaagacct
     9301 ggcggcgcag atcgaaccca tacaccaggc tgtctcggcg ctgggctggc cggtgctggc
     9361 cgtttcgggt gtggaggccg acgacgtgat tggaacactc gcccgtcgcg ccacgcacga
     9421 gggggtgcgt accgtgattt ccaccggcga caaagacctt gctcaactgg tgaatgaaga
     9481 cgtcgagctg gtcaacacca tgagcggtga acgccaggac gtggctggcg tggtgcgtaa
     9541 attcggtgtg gcacctgagc aaattataga cttcctgatg cttaccggcg ataccgtgga
     9601 caatgttccg ggtgtcgaga aagtgggccc caagaccgcg gcgaaatggc tgacggccta
     9661 tcagtccgtc gatgccctgg tcgcccgcgc cgacgaaatc aagggcacgg cgggtcagaa
     9721 cctgcgcaaa gccattcctg gtttcgacct gactcgacga ttgctgacca tcaagaccga
     9781 ttgcgaactg cccatgttgt ctgatggcct gggtgggctc gaattacgtc ctgccgatga
     9841 tcagacgctg atcggcctat acgatcagta tgggttccgc acgtggctgc gcgaactgtc
     9901 cggcgacccc gatcgtgttc ccgcgcagga cgcgcgcgtg gcgccggaag cgcgcgacaa
     9961 ggccgagacg ccagccgata cgcactacga caccattctc gattgggccg cgttcgacca
    10021 gtggctggag cgcatcaaga tggccaacct cgtggctttc gataccgaga ccacatcgct
    10081 cgatcccatg ctggcgcggc tggtggggct gtcgttcgcg acagcgccgg gggaagcctg
    10141 ttacattccg gtggggcatc gcggccccga ccggaccgag cagttgcccc gtgccgaggt
    10201 catcgcccgt cttaccccct ggttgagcga tccccgtgcg gcgaagctgc tgcacaattc
    10261 caagtatgac gcgcacgtgc tccgcaacga aggcatcgcg ctcgcgggca ttgccgaaga
    10321 cacgatgctc caggcctacg tgctggaatc gcatcggcgc gtcaatttgc aggaactggg
    10381 ggagcgctgg ctggggcgca ccggggtcac ctacgaggat ttgtgcggca aaggcgccaa
    10441 acagatcggc ttcgatgagg tcgacatcga aaaggcggcc tattatgcct gcgaagacgc
    10501 ggatttcacc ttgcgtctgc atcatgcctt gcgccctcgc gtcgccgcgg atgccggcct
    10561 cgaaaaaatc tatctgctcg agatcgccac ctcggcggtg ctcaccacca tcgagcgcaa
    10621 cggcgtgcgc atcgatgcgc acgtgctggc catgcaaagc cacgaactcg gcgaacagtt
    10681 gctcgccctc gagaaaaagg cctatgaact cgcggaacag ccgttcaatt tgaattctcc
    10741 caagcaactt ggcgagattc tgttcgagcg catgcggttg ccggtcgtgc gcaagacggc
    10801 ctcaggcgcg ccttccacgg atgaagacgt gctgacccgc ctggcgcacg actacccgtt
    10861 gccgcaagta ctgctcgaat atcgtggcgt ggccaagctc aaatccacct ataccgacaa
    10921 gcttccgcgg atgatcaatc cggcgaccgg ccgtgtgcat acccgatacg cgcaggccgc
    10981 tgtgatcacg gggcgggtgg cgtcgtccga ccccaatctg cagaacatcc cggtgcgcac
    11041 cgccgagggg cgcaaggtgc gcacggcgtt tgtggcgtcg ggcggcgggc agatcgtgtc
    11101 ggccgactat tcccagattg aattgcgtgt catggcgcat gtgtcggatg atgaaaacct
    11161 ccagaatgct tttcggcatg gcgaggatat ccaccgcgcc acggcctccg aggttttcgg
    11221 ggtggcgctc ggcgacgtgt cgtctgagca gcgccgtgcc gccaaggcca tcaacttcgg
    11281 gctgatctac ggcatgagcg aattcggcct ggcgtccagc cttggtatca cgcgcgatgc
    11341 ggcacgggcc tacatggacc gatacttcgc gcggtatccg agagtggccg aatacatgga
    11401 caggacgcgg cataaggcgc gcgagaatgg ctatgtcgaa acggtgtttg gacgacgctt
    11461 gtggctgccg gacatacgcg gcgccaaggg gccgcggttg gcggcggccg agcgcgccgc
    11521 catcaacgcg ccgatgcaag gcacagcggc cgacctgatt aaaatggcta tggtcgctgt
    11581 ccaggaatgg atagcggaac agaagctggc aacgctgatg gtcatgcaag tgcatgacga
    11641 actggtactg gattgtcccg ccgaagaagt cgaaacggtg gttcagcggg tgccggcgct
    11701 catgtgccag gtggccgaac tgcgtgtgcc gctggtcgcc gaggttggcg tcggcgccaa
    11761 ttgggaacag gcgcactaaa tatcatgact cattccgagg gctccatcat gtcgtcaaca
    11821 tccaccatcc atacgtcgtc cgccggactg gaacaaggtc tgttcgatct tcccgcgccg
    11881 gggcagtcga tcaaagcgta tttcgcccgg cccgccggca cgggtgcgtt tcccgtgatt
    11941 ctggtcatcc aggaaatctt cgggttgaat gatcacattc aggatgtttg caggcggttt
    12001 gcgcatcaag ggtatctggc ggttgcggcc gagctcacgc agcggcaggg cgatgcgggc
    12061 tcgtattccg atatcccgac actggttcgc gatgtcgtgg ccaacgtgcc ggacgagcag
    12121 gcgatggccg atctcgacgc ttgcgccgcc tgggccatgg cgcatggtgg ggcggccggc
    12181 caactggggg cgaccggctt ttgctggggt ggccgcatcg cctggttgta cgcggcgcac
    12241 aatccggatt gcaaggcggc ggtcgcgtgg tacggccgca tcgcggcggg gcacggaccc
    12301 ttgcaaacgc atcagcccat ccatctcgcc aatgaattgc atgcgccggt tctgggcttg
    12361 tatggcggca aggatacagg cattcccgtt gacgatgtgc gtgcgctcga ggtcctgctg
    12421 gctcagggca atgacgcggc gcgcgcatcg cggttcaagg tgtacccgga atcgggccac
    12481 gcttttttcg cggattaccg tcctagctat cgtgccgagg atgcgcggga tggttggcgc
    12541 ctggcgctcg actggttcga gggccacgtc agaaaatgac catcggttct ccgtggcact
    12601 cagcgggttg aaatgaccgt cagttttccg tcgcgcgcaa ccggttcggc agcagctcgt
    12661 tgtgccgggc cagtatcggg taggccgccg cgcccaccag ataggaattg atggtcttga
    12721 cgtcgcgcag tatttccagg tagagcgagc cgatttcggc ggattcgatc tggcctgatt
    12781 tgatcttctg caactggcgt tccgcggccg ccgattcgat cgcgcggaat tcgtctttct
    12841 cgaacgccag ttggcgcgcc gacttcaggt cttcggccac gaacagcgcg gcggtctggc
    12901 gttggttgcg gatcagccgg tcgaggacgt gcgtgagttc ctggcgctgt tcgccggtga
    12961 gggaccagcc ttttttcctg agggtcgatg tgtgcccgag caggccgttc gaggtgatgc
    13021 tggccgcatg ggtgatgttg gcgctgaacg tcaggacttc ctccaaccgg cggtggtcgt
    13081 cctggttcat gttttcctga tccagcgtgg ccagatacgc cgtgatgtga ttgtccaggc
    13141 gtgtcaggcc gttgttgatg gtatgggcct gcgcgacccg gtgccggttg tcgcgtagat
    13201 acccggcgcg ggcgatgttg agcatggctt gcagcatgtc cgacaggcgc agtgcttcgc
    13261 gggccgcgtt gcccagggcc acagcgggca cttcgtgcgc ggcttcgtcc agatacatgg
    13321 gtttggacgg atcgttcggg tccacacgct tcggcatcag gcgcgtcagc agtttggcgt
    13381 aaggtttcag gaaaggcatg aacgccacgg cgatcacgac gttgaaaagg gtatggaaat
    13441 tggcgaccgc gcgcgcgggg ctggaggtca gggcgctcat ggcggccggc agccagggca
    13501 gcacaatcat ggccagcacg cagccgacca cccgcgtgcc gagattcccc atgggaagcc
    13561 gccgggctgc cgggtcattg tcgttaaccc cctcgaggat cgggttgatc gcggttccca
    13621 gattggcgcc cagcaccagg gcgtaggcga cgccgggtgc gaccaagccg tgctccgaca
    13681 gggacatgat cagcaccacc acggccacgc tggagtgcgc ggcccaggtc agcacggtgg
    13741 caagcagcag ggcgagaaag ggctgggtgg cgagcgcgtc cagaatggta tggaacaggg
    13801 tcgcgtgctg gatgggggcg aacagagtca ccagttcgtg cagcgacagc agcagcagcc
    13861 cgaggccgat gaacacgcgc ccgagatcgc ggcggcgacc aggctgattg ctgcggaaca
    13921 gccagacgcc caccaggatc agggctggtg ccaacgccgt caggttgaac gatagcacct
    13981 ggacgatcag tgtcgtgccc acgttggcgc ccagcatcgc ggcaaggcct ggcccgagcg
    14041 ccaccagccc gcccgcggcg aacccggtga tcatcaggcc ggttgccgtg ctgctctgca
    14101 gcgcggccgt gatgcccagc cccgccagaa aggcccgcag ccgcgtgccc agcgcatggc
    14161 tcagcgcggt gcccaacgcg gcgccgaatg cgcgttgcac accgctctgg accatgtgca
    14221 cgccccaaag caacagggcg acatagccgg cgaagtcaag cagtgcgagg atttcgctca
    14281 tagcgttgca gtgtagcctg tcccgacttc atgccgggtt caagttgcgc agcgcctgga
    14341 tgcgggctgc gatcggtggg tgggaggcga acagggacgc cgcgcctccg gcggaaatgc
    14401 ccgacgcttc gaacgtcttg ggcaggtcgc ccgactccat gccgccaagc ctggcgaggg
    14461 catggatcat cgggtcgcgc gagcccagca atcgggccga gccggcgtcg gcgcggtatt
    14521 cgcgcaggcg cgagaaccag gcaacaatga tggaagccag gatgccgaag acgatttcgc
    14581 agacgatcac gctcacgtaa tagcccggac ccacgccccg ctcgttcttc agcagcaccc
    14641 ggtccacgaa atacccgacg atgcgcgaca ggaacagcac gaacgtgttg accacgcctt
    14701 gtatcagcgt gagcgtcacc atgtcgccgt tggcgacatg cgacatttcg tgccccagca
    14761 cggcggtgat ctcttcttcg ctcatgctct cgagcaagcc cgttgaaacg gccaccagcg
    14821 cgtcgttctt gaaggcgccg gtcgcgaacg cattgggcgc gccatcgtag atggcgactt
    14881 ccgggtggcc cacgccggcc cgatcggcca actgatgcac cgtatccacc agccacgctt
    14941 cggtggcgtt ggcgggcgcg tgcgggtcga tcacacgggc gccagtgctt tgtttggcca
    15001 tccatttgct gatgagcaga gaaaagatgg cgccggtgaa tccgacgatg gcgcagaaca
    15061 ccagcaattg agacaggttc aggccgttcg cagtgaggaa acggccgacg ccaaagatgc
    15121 tcatgaccac gccgagcacg agcatgacgg cgaagttggt cagcaggaac aggactattc
    15181 gtttcatggt gcgggatccc ctgattcgca tgggtgtctg gcgccgcgat caaccgcgtg
    15241 gagatagggc cgtcagactc tgtaatttgc agagtatagt atcggttttg cggcctttta
    15301 tcctgaattc cggttgatcg catgcagtct ctgtggatgt tgttggcgtg tgccatgttc
    15361 gctgccatgg gtgcctgtgt gaaggtggcg ttcgactatg gcgcgtcgct ccccgagatc
    15421 gtcctgttcc gggg
//
LOCUS       DASTNK010000157         9084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_703594, whole genome shotgun sequence.
ACCESSION   DASTNK010000157 DASTNK010000000
VERSION     DASTNK010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9084
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703594"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /gene="gatB"
                     /locus_tag="VFR20_11830"
     CDS             complement(<1..707)
                     /gene="gatB"
                     /locus_tag="VFR20_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU0231254.1"
                     /translation="MDWEIVIGLETHTQLTTASKIFSDSSTRFGAPPNTQANEIDMAL
                     PGSLPVMNRAAVERAIQFGLAVGAHIAPRSIFARKNYFYPDLPKNYQITQYEIPVVQG
                     GKIHFFVGEREHTVNLTRAHLEEDAGKSLHEHFAGPHGESSTGIDLNRAGTPLLEIVS
                     EPEMRSAAEAVAYARTLHGLVVWLGICDGNMQEGSFRMDANVSVRPRGQQEFGTRSEI
                     KNLNSFRFLERAIQYEVR"
     gene            complement(707..2236)
                     /gene="gatA"
                     /locus_tag="VFR20_11835"
     CDS             complement(707..2236)
                     /gene="gatA"
                     /locus_tag="VFR20_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU0231255.1"
                     /translation="MTMPLHNEFDGIGALRDALAARSVSARELAASALEAATARTDLN
                     AFLHVDPDLTLAQADAADLALARGEGGRLTGIPIAHKDVFVTRGWRTTAGSKMLADYV
                     SPFDATVVERLRQAGAVSIGKLNCDEFAMGSGNQNSAFGPVRNPWDPRAIPGGSSGGS
                     AAAVAAGLVMAATGTDTGGSVRQPSALCGVSGIKPTYGTVSRFGMVAFGSSLDQAGPF
                     ARHARDLLELLDAISGFDPRDSTSLETCDGIANVPGRMRADFDTAMERQSDSGGRPLE
                     GLRIGVPREYFSDGLAADVARAVDAALKVFESLGARRVEISLPRTELAIPAYYVIAPA
                     EASSNLARYDGVRYGHRAEKYGDLNDMISRSRSEGFGPEVLRRILVGTYVLSHGYYDA
                     YYLQAQRVRRMIADDFRQAFDKHCDVIMGPVTPTVAWHIGKNRESPTADWLADIYTLG
                     VSLAGLPAMSVPCGFGGDDQSLPVGLQIIGDYFTEGRLLALADRFQKSTDWHLRKPGQ
                     Q"
     gene            complement(2233..2541)
                     /gene="gatC"
                     /locus_tag="VFR20_11840"
     CDS             complement(2233..2541)
                     /gene="gatC"
                     /locus_tag="VFR20_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU0231256.1"
                     /translation="MAISDQDMARIARLARLELSGPDAARARDELNGILGLIQQLQAV
                     DTSGIEPMAHPLSAHQDVVLRLRADQADATRTPQQRDNLMGNAPATDNGLFLVPTVIE
                     "
     gene            2823..3866
                     /locus_tag="VFR20_11845"
     CDS             2823..3866
                     /locus_tag="VFR20_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEU0231257.1"
                     /translation="MFGFLRSYFSSDMAIDLGTANTLIYVRGKGIVLDEPSVVAIRTD
                     GGASGKKIIQAVGSEAKQMLGRVPGNIEAIRPMKDGVIADFTVTEQMLKQFIRMVHPR
                     SMFAPSPRIIVCVPCGSTQVERRAIRESALGAGASQVYLIEEPMAAAIGAGLGVADAS
                     GSMVVDIGGGTTEVAIISLGGMVYKGSVRVGGDKFDEAIINYIRRNYGMLIGEPTAEL
                     IKKQIGSAFPGSEIREIEVKGRNLSEGVPRSFTVSSNEILESLTDPLNQIVSAVKTAL
                     EQTPPELGADITDKGIALTGGGALLRDLDRLLQEETGLPVLVAEDPLTCVVRGCGEAL
                     EHLERLGAVFIYD"
     gene            3965..4852
                     /gene="mreC"
                     /locus_tag="VFR20_11850"
     CDS             3965..4852
                     /gene="mreC"
                     /locus_tag="VFR20_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU0231258.1"
                     /translation="MQQHGTIRLFKRGPTAEFRLFILVLLSLTLLVIDSRWKVFNPAR
                     QAIAVAIYPFQRLVLAPRDVVARFDDWKDAASLAHTEKEALQRQRIELAQIATHAAQL
                     SAENRQLRRLLKVAETVSQPSVAVEVLYEPPNAFSHHLIFNKGTSSGIRPGMPVIDEG
                     GVVGQIVRVTPFTSEAALLTDEKVSIPVQVLRNGLRLIAFGGNVSGKVEVRYLTADAD
                     IRAGDTLITSGIGGYFPAGLLVARVDRVERDAGSGFALALATPLSHPERYRHFLVLKV
                     DVSEQHAEGAGTDGSKTGR"
     gene            4830..5399
                     /gene="mreD"
                     /locus_tag="VFR20_11855"
     CDS             4830..5399
                     /gene="mreD"
                     /locus_tag="VFR20_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU0231259.1"
                     /translation="MVRKPVDNTTSGRSTLSFLLSLESTVIRRPSSGRMIWGSIFFTW
                     LISLLPWRLWPPAPDLLLLVVLFWCLNEPRRVGMFTAFVLGLLMDVHDAGLLGSHALA
                     YTLAAYGAVTLTRRLLRFNAVVQALHMLPVVLVAEAASHFVHAWLAGEWAGWQWVWSA
                     LLTVALWPLADILLLLPQRRLDDVDPGQG"
     gene            5528..7603
                     /gene="mrdA"
                     /locus_tag="VFR20_11860"
     CDS             5528..7603
                     /gene="mrdA"
                     /locus_tag="VFR20_11860"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU0231260.1"
                     /translation="MFEFKKTSHRQKQKVLTRVWVAGVFALLCFGALLARLWVLQVVR
                     YDGLSARADQNRIAVVPIPPRRGEIVDRNGVVLAKNYRDYTLEVVPAYVKGSMRHMLD
                     TLGTIVYISPRDRRSFLRTLDGAGRYSHILLRNNLNSIEASWFAAHAYEYPGVELRAR
                     WVREYPQGETAAHVVGFVGRISDSDLEELDKEGKAGNYRGTEVIGKRGIEKSYESVLH
                     GRTGVEEVEVTASGRPVRVLHRTDPVPGSNLVLSIDIGLQKVAEEQFKGKRGALVAID
                     PKTGAVLAFVSAPSYDPNLFVDGIDVENWNKLNNDPAHPLIDRPITATYPVGSTYKPF
                     VALAALQMGKRSPTELIPDPGYFEFGGEKFHNAAGEAFGPTDMHKAIVVSSDTYFFSL
                     GPIIGVDALHDFTKQFGFGQLTGIDLDGEKRGVLPSTEWKRHAFKKPSQQRWYAGESV
                     SIAVGQGYDAFTMMQLAHATATLADNGVSMKPHLVSLIENPETGAVTHTVTKPSYTIP
                     LKPENLHIVQDAMKDVVRVGTARNAFKGAPYQAAGKTGTAQVYSLKGVRPHRGQKIPE
                     NLRDHALFIAYAPYDDPRIAVALIVEHGGWGASVAAPIARKLFDYWLSPTRTENENLV
                     SVPPSLAAQTTAYKTARARSPVRTGPPDAVGGDGELIFPGGAEDTDMEHGVDSSAFLS
                     DTSDTGGAK"
     gene            7600..8736
                     /gene="rodA"
                     /locus_tag="VFR20_11865"
     CDS             7600..8736
                     /gene="rodA"
                     /locus_tag="VFR20_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEU0231261.1"
                     /translation="MKQLMRWGKMAVTAFDWPLLALVVLMAIVGMTVMRSAVEGIDWR
                     FADQLRNFAIAFAFMWVAAMLPPPVLMRLAVPFYILGIVLLLGVAFFGETSKGATRWL
                     NIGLTRIQPSEMMKIAVPLMLAWYFHHKQGVMNFLDYVAIGLLLLIPFGLIAKEPDLG
                     TAFLVFTVGFCVIYFGGLSFKLLAPVFVVLAVGVGLLVYYQHDICQPSVHWVVLHDYQ
                     KYRVCTLLDPTSDPLGKGFHTIQSMIAVGSGGIYGKGYMQGTQSHLDFVPERTTDFIF
                     SVFAEEFGLYGGVMLLVLYALIILRGLTITARAPTQFGRLMAGAMAMMFFIYVFVNIG
                     MVTGILPVVGVPLPFMSYGGTALTTLGIACGILMSIRHYRPPVD"
BASE COUNT         1536 a         2720 c         3007 g         1821 t
ORIGIN      
        1 cggacctcgt actggatggc gcgctcgaga aagcggaacg agttcaggtt cttgatttcg
       61 ctgcgcgtgc cgaattcctg ctggcccctg gggcgcaccg agacgttggc gtccatgcgg
      121 aacgaccctt cctgcatgtt gccgtcgcaa atgcccagcc acacgaccaa tccgtgcagt
      181 gtgcgcgcat aggccacggc ttcggcggct gagcgcattt cgggttcgga aacgatttcc
      241 agcagtggcg tgccggcacg gttcaagtcg atgccggtgg acgattcgcc atgcggcccg
      301 gcgaaatgtt cgtgcagcga cttgcctgcg tcttcttcga ggtgcgcgcg ggtcagattg
      361 acggtgtgtt cccgttcgcc cacaaagaaa tggatctttc caccctggac caccggaatc
      421 tcgtactgtg tgatttggta gtttttcggg aggtcggggt agaagtagtt cttgcgggcg
      481 aagatcgagc gcggtgcgat gtgggcgccg accgccaggc cgaactggat cgcgcgttcg
      541 accgcggcgc ggttcatgac gggcaggctg ccgggcagcg ccatgtcgat ctcgttggcc
      601 tgcgtgttgg gtggcgcacc gaagcgagtg ctgctgtcgg agaaaatctt ggatgccgtg
      661 gtgagctggg tatgagtctc cagcccgatg acgatttccc aatccattat tgttgtcccg
      721 gtttgcgtag atgccagtcg gtcgatttct ggaaacggtc ggcgagggcc agcagccggc
      781 cttcggtgaa ataatcaccg atgatctgca gccccacagg caacgactgg tcgtcgccgc
      841 caaaaccgca agggacggac atcgccggca ggcccgccag gctgacgccc agggtataga
      901 tgtcggccag ccaatcggcg gttggggatt cgcggttctt gcctatgtgc caggccaccg
      961 ttggcgtgac ggggcccatg atgacgtcgc aatgcttgtc gaacgcctgc cggaaatcgt
     1021 ctgcgatcat gcgccgcacg cgctgggcct gcaggtaata cgcgtcgtag tagccgtggg
     1081 atagcacgta cgtgcccacc aggatccggc gcaggacttc cgggccgaag ccctcgctgc
     1141 gcgagcggct gatcatgtcg ttcaggtcgc cgtatttttc cgcgcggtgc ccatagcgca
     1201 cgccatcgta gcgcgccagg ttgctggatg cctcggccgg ggcgatgacg taataggccg
     1261 ggatcgccag ttccgtgcgc ggcagcgaga tttccacccg acgggcgccc agcgactcaa
     1321 aaactttcag cgcggcatcc acggcgcgcg ccacgtcggc ggcgagaccg tccgagaagt
     1381 actcacgggg aacgccgata cgcaggcctt ccagggggcg gccaccggag tcggattggc
     1441 gttccatggc cgtgtcgaag tcggcgcgca tgcgtccggg aacgtttgcg atgccatcgc
     1501 aggtttccag gctggtggaa tcacgcgggt cgaagccgct gatcgcatcg agcagttcga
     1561 gcagatcacg agcgtggcgc gcgaacggac ccgcctgatc gagactggag ccaaaggcga
     1621 ccatgccgaa acgcgagacg gtgccatagg tgggcttgat gccgctgaca ccgcacagcg
     1681 cggagggctg gcgcacggaa ccgcccgtgt cggtgcccgt ggcggccatg accaggccgg
     1741 cggccaccgc ggcggccgac ccgcccgagg agccgcccgg aatggcgcgg ggatcccagg
     1801 ggttgcgaac cgggccgaac gccgagttct ggttacccga gcccatcgcg aattcgtcgc
     1861 aattgagctt gccgatcgac actgctccgg cctgccgcag acgttcgacg accgtggcgt
     1921 cgaacgggct gacatagtcg gccagcatct tgctgccggc ggtggtgcgc caaccgcgtg
     1981 tcacgaacac gtctttgtgg gcgatgggaa tgccggtgag gcggccaccc tcgccgcgcg
     2041 ccagcgccag atcggcggcg tcggcctgtg ccagggtgag gtccggatcg acgtgcagga
     2101 aagcattcag gtcggtgcgc gccgtcgccg cttccagggc gcttgcggcg agttcccgcg
     2161 cactgactga gcgggctgcc agggcatcgc gcaaggcgcc gataccgtcg aattcgttgt
     2221 gcaggggcat ggtcattcta tgacggtggg aaccaggaac aggccgttgt cggtggcggg
     2281 ggcattgccc atgaggttgt cgcgctgttg cggtgtgcgc gtggcatcgg cctggtccgc
     2341 gcgcaggcgc agcacgacat cctggtgcgc ggacaagggg tgcgccatgg gttctatgcc
     2401 agaggtgtcg accgcttgca attgctggat gagcccgagg atgccgttga gttcatcccg
     2461 ggcgcgcgca gcgtccggcc cggaaagttc gagacgcgca agccgcgcga tgcgcgccat
     2521 gtcctggtcg ctgatagcca tgaatgtgtc tggtcgtaaa atcgccctgg ccgcgaatcg
     2581 gtgtatttgt taaacaaatt gcaacttcaa gcggttggca ggggcggata gatcaaaata
     2641 agctgctgtt cggcttattg ccgaatagat tataagttat cattacgggt ttcaatcgtt
     2701 ggctgatttc cccctttgcg gctgggcggg aaatcgcctg tcgcgcggcc ggccgctggc
     2761 atgaacgcgg ggccgatcgc ttctcgcgac gcttccaaat ctcgacttca cactgagcac
     2821 tcatgttcgg atttcttcgc agttacttct ccagcgacat ggccatcgac ctggggaccg
     2881 ccaacaccct catttacgtc cgcggcaagg gcattgtgct cgatgagcca tcggtcgtcg
     2941 ccattcgcac cgatggcggc gccagtggca agaaaatcat ccaggcggtc ggcagcgagg
     3001 ccaagcagat gctgggtcgc gtgccgggca acatcgaagc catccgcccg atgaaagacg
     3061 gggtcatcgc ggacttcacc gtcaccgagc aaatgctcaa gcagttcatc cgcatggtgc
     3121 atccgcgcag catgttcgcg cccagtcccc gcatcatcgt ttgcgtgccc tgcggttcca
     3181 ctcaggtcga gcgccgcgcc atccgcgaat cggcgttggg ggcgggggcc agtcaggtgt
     3241 acctcatcga agagcccatg gccgcggcca tcggcgcagg cctgggggtg gccgacgcca
     3301 gcggttccat ggtcgtggac atcggcgggg gcaccaccga ggtcgccatc atttccctgg
     3361 gcggcatggt gtacaagggt tcggtgcgcg ttggcggcga caagttcgat gaggccatca
     3421 tcaactacat ccgccgcaac tacggcatgc tcatcggcga gcccaccgcc gaactcatca
     3481 agaagcaaat cggctcggcc ttcccaggct cggaaatccg tgagatcgaa gtcaagggcc
     3541 gcaacctgtc cgaaggtgta ccgcgcagct tcacggtgtc ctccaacgaa atcctggaat
     3601 cactcaccga tccgctcaat cagatcgtct cggcggtcaa gacggcgctg gagcagaccc
     3661 ctcctgaact gggcgccgac atcaccgaca agggcattgc cctgaccggc ggcggggcgc
     3721 tgttgcgcga tctcgatcgg ttgctgcagg aagaaaccgg cttgccggtg ctggtggcgg
     3781 aagatcctct cacttgcgtc gtgcgcggct gcggcgaggc gttggagcac ctcgagcgtc
     3841 tgggagccgt gttcatttac gattaaggcc tgctcctatg cggacaggcc tgaatcctcg
     3901 gctgtcgggt cgggtgccat gtcatcggtg cgccggcgca ttccggcgcc attgagggca
     3961 gctcatgcaa caacacggga cgatcagact cttcaagcgc ggaccgacgg cggagttccg
     4021 cctgttcatc ctggtcctgc tgtcgctgac gctgctggtg atcgattccc gctggaaggt
     4081 tttcaatccg gcccgccagg cgatcgcggt ggcgatctat ccctttcagc gcctggttct
     4141 cgcaccacgc gacgtcgtgg cgcgtttcga cgactggaag gatgccgcgt cgctggcgca
     4201 cacggaaaaa gaggcattgc agcgtcagcg catcgagctc gcccagatcg ccacgcacgc
     4261 ggcccagctc tcggccgaga accggcagtt gcggcgcctg ctcaaggttg ctgaaacggt
     4321 ctcgcagcct tcggtggcgg tggaagtgct ctacgagcca cccaatgcgt tttcccacca
     4381 cctgattttc aacaagggga cgtccagcgg catccggccc ggcatgccgg tgatcgacga
     4441 gggcggtgtg gtgggccaga tcgtgcgggt gacgccattc acgtcggaag cggcgttgtt
     4501 gacggacgaa aaggtgtcga tcccggtgca ggtcctgcgc aatgggctgc gtctcattgc
     4561 gttcggcggc aatgtgtcgg gcaaggtcga ggtccgctac ctgacggcgg atgccgacat
     4621 ccgcgctggc gacaccctga tcaccagcgg cataggcggg tatttcccgg ccgggctgct
     4681 ggtcgccagg gtcgatcgcg tcgagcgtga cgccggatcg gggttcgcgc tcgcgctggc
     4741 cacgccgttg tcgcaccccg agcgctaccg gcattttctg gtgttgaagg tggatgtctc
     4801 ggaacaacat gccgaaggag cgggaaccga tggttcgaaa accggtcgat aatacgacgt
     4861 ccggacgctc cacgctgagt ttcctgttgt ccctggagtc cacggtcatc aggcgtccgt
     4921 ccagcggccg gatgatctgg gggtcgatct tcttcacctg gttgatctcc ctgttgcctt
     4981 ggcggctatg gccgccggcg ccagacctgc tcttgctggt ggtgctgttc tggtgtctga
     5041 atgagccgcg cagggtgggt atgttcacgg cgttcgtcct ggggttgctc atggacgtgc
     5101 acgacgcggg cctgctcggc agtcacgcgc tggcctatac gctggcggct tacggcgcgg
     5161 tcaccttgac gcgacgactg ctgcggttca acgcggtggt gcaggcgttg cacatgctac
     5221 ccgtggtgct tgtcgccgaa gccgcctcac acttcgtcca tgcgtggttg gcgggcgaat
     5281 gggcgggttg gcaatgggtc tggtcggcgc tcttgacggt ggccctgtgg ccgctcgccg
     5341 acattctgtt gttgttgcct caacggcgcc tcgacgatgt ggaccccgga caggggtgac
     5401 cctcggcagg ccgtgaccgg tattcctggg ggcgcagcgg cggtatgccg ttttttgtga
     5461 tcagttcttt cagtcatatg gcaatcggtt agcctgaacg ggcctcgatc cgcctcgtca
     5521 cccatttatg ttcgagttca agaagacgtc gcatcggcag aaacagaagg tcctgacccg
     5581 ggtctgggtc gcgggcgttt tcgcgttgct gtgtttcggt gccctgctcg ctcggctttg
     5641 ggtgttgcag gtggtgcgct atgacggcct gtcggcgcgc gctgatcaga accgcattgc
     5701 cgtggtcccc attccgcccc ggcgcggcga gatcgtcgac cgcaatggtg tggtgctggc
     5761 caagaattat cgcgattaca cactggaggt ggtccccgcg tatgtgaagg gctccatgcg
     5821 ccacatgctc gacacgcttg ggaccatcgt ctacatcagt ccgcgcgatc ggcgcagctt
     5881 tttgcgcaca ctggacggtg cggggcgtta ttcgcacatc ctgctgcgca acaacctgaa
     5941 cagcatcgag gcatcgtggt tcgcggccca cgcatacgag tatccgggtg tcgaactgcg
     6001 cgcgcgatgg gtgcgggaat atcctcaagg tgaaaccgcg gcgcacgtgg tgggtttcgt
     6061 ggggcgcatt tccgactccg acctggagga actcgacaag gaaggcaagg cgggcaacta
     6121 tcgcggcacc gaggtcatcg gcaagcgtgg tatcgaaaaa tcctatgagt cggtgctgca
     6181 tggccgcacc ggtgtcgagg aggtcgaggt cacggcgtca ggccgtccag tgcgtgtgct
     6241 gcatcgcaca gaccccgttc ccggttccaa tctggtgctc tccatcgaca taggcctgca
     6301 gaaggtggcc gaggagcagt tcaagggcaa gcgtggcgcc ctggtcgcca ttgatcccaa
     6361 aacgggcgcc gtgctggctt ttgtgtcggc gccttcgtat gatcccaacc tgttcgtcga
     6421 cggcatcgac gtcgaaaact ggaacaagct caataatgat ccagcccatc cactgatcga
     6481 tcggccgatc accgccacat accccgtggg ttcgacgtac aagcctttcg tcgcactggc
     6541 ggcgctgcaa atgggcaaac ggtctccgac cgaactgatt cccgatccgg ggtatttcga
     6601 attcggtggc gagaagttcc acaacgcggc gggggaggca ttcgggccga ccgacatgca
     6661 caaggccatc gtcgtgtcgt ccgacaccta tttcttctcg ctgggcccca tcatcggtgt
     6721 cgatgccttg cacgatttca ccaagcagtt cgggttcggg cagcttaccg gcatcgatct
     6781 ggatggcgag aaacgcggtg tgctgccttc cacggaatgg aagcggcacg cgttcaagaa
     6841 gccatcgcag caacgttggt acgcggggga gtccgtgtcg atcgcggtgg gccagggata
     6901 cgatgcgttc actatgatgc aactggccca cgccaccgcc acgcttgccg acaacggcgt
     6961 cagcatgaaa ccgcatctcg tcagcctgat cgaaaacccc gagacaggcg cggtgaccca
     7021 caccgtgacc aagccgtcct acacgattcc gctcaagccc gagaatctgc atattgtcca
     7081 ggacgcgatg aaggacgtgg tgcgcgtcgg cactgcgcgc aatgcgttca aaggggctcc
     7141 ctatcaggca gcgggcaaga ccggtacggc ccaggtgtat agcctgaaag gcgtgcgtcc
     7201 gcatcgcggc cagaagatac ccgagaacct gcgcgaccac gcgttgttca tcgcctatgc
     7261 accgtatgat gaccctcgca tcgcggtcgc gctcatcgtc gagcacggtg gctggggagc
     7321 gtcggtcgcc gcgccgatcg cgcgcaagct tttcgattac tggttgtctc cgacgcgcac
     7381 cgagaatgaa aacctggtca gtgtcccgcc ctcgctggcg gcgcagacca cggcgtacaa
     7441 gacggcgcgg gcccgttccc ccgtgcggac cggccccccc gacgcggtcg gtggcgatgg
     7501 tgagctgatc ttcccgggtg gagccgagga tacggatatg gaacatggtg tggattcctc
     7561 tgcgttcctg tcagacactt ccgatacggg aggcgcgaaa tgaagcaatt aatgcggtgg
     7621 gggaagatgg ccgtgacggc gttcgactgg ccgttgctcg cgctggtcgt gctgatggcc
     7681 atcgtgggca tgactgtcat gcgctcggcg gtggaaggca tcgattggcg cttcgccgac
     7741 cagttgcgca atttcgccat cgccttcgcc ttcatgtggg tggcggccat gttgccgccg
     7801 ccggttctga tgcgcctggc tgtgccgttc tacattctcg gcattgttct gctgctgggg
     7861 gtcgccttct tcggcgaaac cagcaagggc gcgacgcgct ggttgaatat cggtctcacg
     7921 cgaatacagc cctctgaaat gatgaagatc gccgtgccgc tgatgctcgc ctggtatttt
     7981 catcacaagc aaggggtgat gaatttcctc gattacgtgg ccatcggctt gctgttactc
     8041 atcccgttcg gcctgatcgc gaaagagcct gatctcggca cggccttcct ggtgttcacg
     8101 gtggggttct gcgtgattta tttcggcggc ctgtcgttca agctgcttgc gccggtgttc
     8161 gtggtgctgg cggtgggcgt gggtttgctg gtgtattacc agcacgacat ctgtcagcct
     8221 agtgtgcact gggtcgtgtt gcatgactat cagaagtacc gggtgtgcac gttgctggat
     8281 cccacctccg atccgctcgg caagggtttt catacgatac agtcgatgat cgcggtgggt
     8341 tcgggcggta tctatggcaa gggctacatg cagggcacgc agtcgcatct ggatttcgtg
     8401 ccggagcgca ccaccgattt cattttctct gttttcgccg aggagttcgg gctctatggc
     8461 ggcgtcatgc tgctggtgct gtatgcgctg atcatcttgc gcggattgac gatcaccgcc
     8521 cgggcgccca cgcagttcgg gcgtctgatg gccggcgcca tggcgatgat gtttttcatt
     8581 tatgtgttcg tcaacatcgg gatggtgacg ggtattcttc cggtggttgg tgtgccgttg
     8641 ccgttcatga gttatggggg gacggcgttg accacgctgg gcattgcctg cgggatactg
     8701 atgagcatcc gccattatcg gccgccggtg gattgagctg ggggcgcttt tggtatctta
     8761 agacgctggc tatcggggcc aggggcgtgc ccggcacggc gtgcttcatc cggatctgtc
     8821 tcgtccaggc gggcgtcggg cgaaactcgc cgcgccctgc gggcacggct caaacagtcg
     8881 cccgacgact gcccccgcct gaccaagcca gatccggcgc acgccttaca ccgggcccac
     8941 cccaggcccc gatagacagc tcgcgttggt gatatgcggg ggagccaaca agcccatacc
     9001 ggggcaatag ccttccaagc atgccgctgt cgtagcccca gggtggggga ggtggcgcag
     9061 gccggcgttt cggccagcgc cgga
//
LOCUS       DASTNK010000158         3284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_333088, whole genome shotgun sequence.
ACCESSION   DASTNK010000158 DASTNK010000000
VERSION     DASTNK010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333088"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VFR20_11870"
     CDS             complement(<1..512)
                     /locus_tag="VFR20_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231262.1"
                     /translation="MGLILLAIPAALDNRRTGQTGIQRLLLNIEALDDQTFVCCKALP
                     ACAGMTNTKPVVQTRPDAASHAYQPATVTIIKTRAACQPAHNQWPASLPHQVPAGVSP
                     KQPRTHRTTALAAGSPAIKPALAIPSYPCQLTLHEKGIAMDKVYPSATKALDGLLFDD
                     MTIAAGGFGL"
     gene            643..1140
                     /locus_tag="VFR20_11875"
     CDS             643..1140
                     /locus_tag="VFR20_11875"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0231263.1"
                     /translation="MTKAGCWFEDVVLPALLRHARVTYGTAMRRALADAGYDDLPRNG
                     LYVIGGLALGAGDAPLGALIKELRISKQAAGQLVDTLVSRGYLQRTIDEHDRRRLTVT
                     LTERGRDAAKTQAIARKAIDAELTTRVGESGVKAARRALAALIDIGREREALDERATG
                     GAEES"
     gene            1137..1517
                     /locus_tag="VFR20_11880"
     CDS             1137..1517
                     /locus_tag="VFR20_11880"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0231264.1"
                     /translation="MKITHVITQLRTTDLAASIGFYTNTLGLTLAFEHQDFYAGIRSG
                     NQIFHLKLADEKDPSIEAVAKDGHFHLYLETDDAAAAAVTLKSRGVRLVEDLHDTSWG
                     TREFVIRDDQGHTLYFGENRDGAS"
     gene            1644..2063
                     /locus_tag="VFR20_11885"
     CDS             1644..2063
                     /locus_tag="VFR20_11885"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0231265.1"
                     /translation="MSSQFVAAAVIYAKDMLRISKFYEAIACLVVVETGEGYIVLEGG
                     GFQLSVVAIPANIAATVKVSDPPTRREGSPIKLVHFVQSIAAARKQTTELGGHLNSAA
                     REWKFQGVRVCDGHDPEGNVFQLRENPLANSVAECNG"
     gene            complement(2409..2879)
                     /locus_tag="VFR20_11890"
     CDS             complement(2409..2879)
                     /locus_tag="VFR20_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231266.1"
                     /translation="MRRPALIAALALTLALPALAQADTLTALTPAEVPALLKPPAHGE
                     RILAIWALDCAYCESNMQALAKLQQAHPHEITLVTVATDDIAQRQAIEQRLQHAGLDN
                     YPARAYAAITPDRINYLLDPSWGGETPRTLLIRADGTHLGISGELTPQQLQKLH"
     gene            complement(2882..>3284)
                     /locus_tag="VFR20_11895"
     CDS             complement(2882..>3284)
                     /locus_tag="VFR20_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEU0231267.1"
                     /translation="ACPHQGPGLAIGADGVRHAVWYEAKNGPTIWYGQLDPDHPAKHK
                     LKIGGAGAGHADVAAHDHTVWVAWNQVDAKGYTLMLRRSTDNGVHFDAPRAISHIDGA
                     AGSPQLLQWQGRAYVAWNTSAGFRLIPAEVQ"
BASE COUNT          618 a          896 c         1112 g          658 t
ORIGIN      
        1 cagaggccga agccgccggc ggcgatggtc atgtcgtcga acagcaaacc atcaagagcc
       61 ttcgttgcac ttgggtaaac cttgtccatc gcgatgcctt tctcgtggag agtcagctga
      121 caaggatacg acggaatggc caatgcgggt tttatggccg ggctgcccgc agccagcgcc
      181 gttgttcgat gcgtacgggg ttgcttcggg ctgacgccgg caggaacttg gtgcggcagg
      241 cttgcaggcc attgattgtg ggcaggctgg catgcggctc tggttttgat gatcgtgacg
      301 gtcgccggct gatatgcgtg gctggcagca tcagggcggg tctgaacaac cggctttgtg
      361 ttcgtcattc ctgcgcaagc agggagtgct ttacaacaga cgaaggtttg gtcatccagt
      421 gcctcgatgt tcaatagaag gcgctggatc ccggtttgac cagtccttcg gttgtcgaga
      481 gccgctggga tggcgagcag aatcagaccc accttggcga aatcatcacg gctggcacgc
      541 ggcgctggtt gaggaggcac ggtggggcgt cgcgaggcta ttgacgtggt caattggtat
      601 tgtatattta tagtcaataa caagtactaa atgaagatgg ccatgaccaa agctggctgc
      661 tggtttgaag acgtcgtgct accggctttg ttgcgacatg ctcgggtcac ctatggcacc
      721 gcgatgcgcc gtgctctggc cgacgccggt tacgatgatc tccccaggaa tggcctgtat
      781 gtcatcggtg gcctggcgtt aggtgcgggt gatgcgccgt tgggcgcgct catcaaggag
      841 ctacggatct ccaaacaggc ggcaggtcag cttgtcgaca ccttggtgtc gcgcggttac
      901 ctgcaacgca ccatcgacga acacgaccgc cgcagactga ccgtgaccct gaccgaacgc
      961 gggcgcgatg cggcgaagac tcaagccatt gcccgcaagg ctatcgatgc cgaactgacg
     1021 acccgcgtgg gcgaaagcgg tgtaaaggcc gcgcgtcgag cgctggcggc gttgatcgac
     1081 attggacgcg agcgcgaagc cctggacgaa agggcaaccg ggggagccga ggaatcatga
     1141 aaattacgca tgtcattacc caactccgca cgaccgacct ggctgcgtcg ataggttttt
     1201 acacgaacac gctgggtctg acgctggctt tcgagcacca ggacttctac gccgggattc
     1261 gatccggaaa tcagatattc catctgaaac tggcagacga gaaagatcct tcgatcgagg
     1321 ccgtcgccaa ggacgggcac ttccatctct atctggagac tgatgatgcc gcagctgcgg
     1381 ctgtgaccct gaagagtcga ggggtccggc tggtggagga cttgcacgac accagttggg
     1441 gcacacggga gtttgtcatt cgggacgatc aaggacatac cttgtacttc ggcgaaaacc
     1501 gcgatggcgc ctcctgatgc gatcaagctg aggggcgatc aactgtcgcc atcccgcgca
     1561 actatccgac cggcggctgc cagccgacgt ccgcattcaa gcccggatgg ccgtccagtc
     1621 atgactcgta caaggatcag cagatgtcta gccaattcgt tgcggccgca gtcatctatg
     1681 cgaaggatat gcttcgtatc agcaagttct acgaagcgat cgcctgtctt gtcgttgttg
     1741 aaaccgggga agggtacatc gttctggaag gcggcggatt ccagctttca gtcgtggcaa
     1801 tcccggcgaa tatcgccgcc acagtgaaag tcagcgaccc gccgacaagg cgggaaggtt
     1861 caccgatcaa gttggtccac ttcgttcaaa gcatcgcggc cgcgcggaaa cagacaaccg
     1921 aactcggcgg gcatttgaac tcggccgcgc gcgagtggaa atttcaaggt gttcgcgttt
     1981 gcgatggaca tgatccggaa ggaaatgttt ttcagctccg ggaaaatccg ttggcgaact
     2041 cagttgcaga atgcaacgga tgagaaatac ccgatcattt gcaatggccg tcttcgggtt
     2101 ggctctgcag taatcgagtg gccgttgttg gcgggcggaa ggttggttgg cccggtacgt
     2161 gtcatggcga tggtgggcgg gtggctggtg tcttgcagat cacggatctt catgaagatg
     2221 ggacgccgtt tgcatggcta tggcagggag gcgactcgaa tcggctggcg gtgaccaatc
     2281 atgcccgtca tcctggcgaa ggccggaatg acgggaaaaa gcccgatttc agcatgtgta
     2341 gcgcatggct gcaggtgtcc aacatcatcc tggacagcag tgcgtatccg gcggtgtggg
     2401 ccgaaccgtc aatgcagctt ctgcagttgc tgcggcgtca gttcaccgct gatgccgagg
     2461 tgcgtgccat cggcgcggat cagcagtgtg cgcggggttt cgccgcccca gctgggatcg
     2521 agcaggtagt tgatgcggtc cggtgtgatg gcggcgtagg cgcgggcggg gtagttgtcc
     2581 aggcctgcgt gctgcaggcg ttgctcgatc gcctggcgtt gcgcgatgtc gtcggtggcg
     2641 acggtgacca gggtgatctc gtgcggatgg gcctgctgca gtttcgccag cgcctgcatg
     2701 ttcgattcgc agtaggcgca gtcgagcgcc cagatggcga ggatgcgttc accgtgggcc
     2761 ggtggcttca gcagtgcggg cacctcggcg ggcgtgagcg cggtgagggt gtccgcctgc
     2821 gcgagggcgg gtagcgccag ggtgagtgca agcgcggcga tgagtgcggg gcggcgcatg
     2881 gtcactgcac ctcggcaggt atcagccgga agccggcgct ggtgttccac gccacgtagg
     2941 cgcgcccctg ccactgcagc agttgcggcg agccggcggc gccatcgatg tgactgatcg
     3001 cacggggcgc gtcgaagtgc acgccgttgt cggtcgagcg gcgcagcatc agcgtatagc
     3061 ccttggcatc gacctggttc cacgccaccc acacggtgtg atcgtgcgcg gcgacatcgg
     3121 catggccggc accggcaccg ccgatcttca gcttgtgctt cgccggatgg tcgggatcaa
     3181 gctggccgta ccagatcgtg gggccgttct ttgcttcgta ccagaccgcg tggcgtacgc
     3241 catcggcacc aatggcgagg ccgggcccct gatgcgggca tgcg
//
LOCUS       DASTNK010000159         3921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1630330, whole genome shotgun sequence.
ACCESSION   DASTNK010000159 DASTNK010000000
VERSION     DASTNK010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630330"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VFR20_11900"
     CDS             complement(<1..319)
                     /locus_tag="VFR20_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase domain-containing protein"
                     /protein_id="HEU0231268.1"
                     /translation="MPQQTPEPQAVSSPVTHSVIFLVATLAAGEEAAATVRAWCADIA
                     ALVRSVGKRVPSGNLSCVCGFSAGAWDALFGEPRPASLHPFREVGVPGRLAVATPGDI
                     LLHI"
     gene            508..1572
                     /gene="ruvB"
                     /locus_tag="VFR20_11905"
     CDS             508..1572
                     /gene="ruvB"
                     /locus_tag="VFR20_11905"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU0231269.1"
                     /translation="MAIHSDSLSGSAIPERIVAPQTASPNEESIERALRPRAFGDYVG
                     QDRVREQLEIFISAARNRQESLDHVLLFGPPGLGKTTLAHIVAHEMGVQLRQTSGPVL
                     ERAGDLAALLTNLEKNDVLFIDEIHRLSPVVEEILYPALEDFQIDILIGEGPSARSVK
                     IDLQPFTLVGATTRAGMLTNPLRDRFGIVARLEFYDTNDLARIVMRSAHLLNADATPD
                     GAREIARRARGTPRIANRLLRRVRDYAEVKANGRIDADTANAALSMLEVDPQGLDLMD
                     RKLLEAIIHKFDGGPVGVDSLAAAIGEERDTIEDVIEPYLIQNGYLQRTARGRIATQS
                     TWRHLGLTPPSAPDTPDLFN"
     gene            complement(1565..3415)
                     /gene="recQ"
                     /locus_tag="VFR20_11910"
     CDS             complement(1565..3415)
                     /gene="recQ"
                     /locus_tag="VFR20_11910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEU0231270.1"
                     /translation="MPSSSPSTPPRPSTALDVLRRIFGYESFRGEQQTIIEHLIDGGD
                     ALVLMPTGGGKSLCYQIPALVRDGVGVVVSPLIALMQDQVSALVEQGVRAAFLNSTQD
                     WREAREVEQEFLAGRIDLLYVAPERLLTDRCMELLSRGRIALFAIDEAHCVSQWGHDF
                     RPEYLGLSILAERWPQVPRIALTATATAATRLEIVQRLELGSARQFVASFDRPNIRYR
                     IVEKNEVRRQLLAFIQAEHTGDCGIVYCLSRARVEETAEFLCDQGIEALPYHAGLSAS
                     VRAANQSRFLREDGLVMVATIAFGMGIDKPDVRFVAHIDLPKSVEGYYQETGRAGRDG
                     QPATAWLAYGLQDVVQQRRMIDESPGDDAFRRRLGAQLDAMLGLCETVSCRRQRLLAY
                     FDQSLEPCGNCDTCLEPPQAWDGTVAAQKVLSAVYRLWRERGQRFGAGHLIDILRGKL
                     TARIEQYGHNSLSVFGVGADLSEQVWRGVLRQLLAQRLLAVDHEGYGTLGLTDDSRSV
                     LKGERTLMLRREPEKKARGARVPSKRAKEDVLLPPDAQTRFEALRAWRTEVARSHGVP
                     AYVIFHDATLREIALHRPTSLDELGHVSGVGARKLEAYGADLLDCVDGVS"
     gene            complement(3621..>3921)
                     /locus_tag="VFR20_11915"
     CDS             complement(3621..>3921)
                     /locus_tag="VFR20_11915"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU0231271.1"
                     /translation="RRRVQRSGINTECKLMLLTHAFEELDCIAVEWRTHFLNHQSRAG
                     IERLGAKLDGVLRQHQISPNGTLRDTCVYSIIASEWPTVKAHLDHQLSKPRAALS"
BASE COUNT          754 a         1398 c         1162 g          607 t
ORIGIN      
        1 ggatgtgaag caggatgtcg cccggcgtgg caaccgccaa gcggcccggc acgcccacct
       61 cgcggaaggg atgcaacgaa gccggacgcg gttcgccaaa caacgcatcc caggcaccgg
      121 cactgaaccc gcacacgcac gacaggttgc ccgacggcac acgcttgccg acggaacgca
      181 ccagcgcggc aatatcggcg caccaggcgc gcaccgtggc agcggcctct tcccccgccg
      241 cgagcgtggc gacaaggaaa atcacgctat gcgtaacagg gctggaaaca gcttgtggtt
      301 caggagtttg ctgaggcatg gaatccggca agaatgaggt gtcgtttaca aatcatgcct
      361 gtcacacatc aggtcgtcaa ggtgccggcc cgagcgaaaa tactgttatc atgtacagta
      421 tcagacacag gccacccacc tgacccgctc cgggctcgcc cccgcaaccg gtaaaatccc
      481 cacggtaccc attcaaactc gtccgccatg gccatacact ccgattcgct gtccggctcc
      541 gccattccgg aacgaatcgt cgccccgcaa accgcctcgc ccaacgaaga atccatcgag
      601 cgcgcgctac gcccccgggc cttcggtgac tacgtcgggc aagatcgcgt gcgcgaacag
      661 cttgaaatct tcatctccgc cgcccgcaac cgccaggaaa gcctcgatca cgtcctgctc
      721 ttcggcccgc cgggcctggg caagaccacc ctggcccaca tcgtcgctca cgaaatgggc
      781 gtgcagttgc gccagacatc gggccccgtg ctcgaacgcg ccggcgacct cgccgcgctg
      841 ctcaccaacc tcgaaaagaa cgacgtcctg ttcatagacg aaatccaccg gctgtcgccc
      901 gtcgtcgagg aaatcctcta ccccgccctc gaagacttcc agatcgacat cctcatcggc
      961 gaaggcccgt cggcgcgcag cgtcaagatc gacctgcagc cgttcaccct ggtcggcgcc
     1021 accacccggg ccggcatgct caccaacccc ctgcgcgacc gcttcggcat cgtcgccagg
     1081 ctcgagttct acgacaccaa cgacctggcg cgcatcgtca tgcgcagcgc tcacttgctc
     1141 aacgccgacg ccacccccga cggcgcccgc gaaatcgcgc gccgcgcgcg tggcacaccg
     1201 cgcatcgcca accgcctgct gcgccgcgtc cgcgactacg ccgaagtcaa ggccaatggc
     1261 cgcatcgacg ccgacaccgc caacgccgcg ctgtccatgc tggaagtcga cccccagggc
     1321 ctcgatctca tggaccgcaa gctgctcgaa gccatcatcc acaagttcga cggcggcccc
     1381 gtcggcgtcg acagcctcgc cgccgccatc ggcgaagagc gcgacaccat cgaagatgtc
     1441 atcgaaccct atctcatcca gaacggctac ctgcagcgca ccgcccgcgg ccgtatcgcc
     1501 acgcagtcca catggcggca cctggggctg acgccgccat ccgcgccaga cacccccgac
     1561 ctgttcaact gacgccgtcc acacagtcca acaggtcggc gccgtaggct tccagcttgc
     1621 gcgcacccac gccgctcacg tgccccaatt cgtccagcga ggtcgggcga tgcaacgcga
     1681 tctcgcgcag cgtggcgtcg tgaaagatca cataggccgg cacgccatgg ctgcgcgcca
     1741 cctcggtgcg ccaggcgcgc agcgcctcga accgggtctg ggcgtccggg ggcagcagaa
     1801 cgtcttcttt cgcgcgcttg gatggcacac gcgcaccccg ggctttcttt tcaggctcac
     1861 gcctgagcat cagtgtccgt tcgcccttga gcactgagcg gctgtcgtcc gtcaggccca
     1921 gcgtgccgta cccttcgtgg tccacggcaa gcaagcgttg cgccagcaat tggcgcaaca
     1981 ccccgcgcca gacctgctcg gagagatccg cccccacacc gaacaccgac agcgaattgt
     2041 ggccatactg ctcgatgcgc gccgtgagct tgccgcgcag gatgtcgatc aggtgtcccg
     2101 ccccgaaacg ctggccgcgt tcgcgccaca gccgataaac ggcggacagc accttctgcg
     2161 cggcgacggt gccatcccac gcctgcggcg gctccaggca cgtgtcgcag ttgccgcagg
     2221 gttccaggga ctgatcgaaa tatgccagca gtctttgacg gcggcaggac acagtctcgc
     2281 acaggcccag catggcatcg agctgcgccc ccaggcggcg ccggaacgca tcgtcgcctg
     2341 gcgactcatc gatcatgcgc cgctgctgga ccacatcctg caggccatat gccagccagg
     2401 cggtggcggg ctgcccgtcg cggcccgcgc gcccggtttc ctggtaatac ccttcaaccg
     2461 acttgggtag atcgatgtgc gccacgaagc gcacgtcggg cttgtcgatg cccatgccga
     2521 acgcgatggt ggccaccatc accaggccgt cctcgcgcaa aaagcgcgac tggttggcgg
     2581 cgcgcaccga tgcgctcaac ccggcatgat acggcagcgc ctcgatgccc tggtcgcaca
     2641 ggaactcggc ggtttcctcc acgcgcgcgc gtgacagaca gtacacaatg ccgcagtcgc
     2701 cggtatgctc cgcctggatg aacgccagca actggcgacg cacctcgttc ttttccacga
     2761 tgcggtaacg gatgttcggc cgatcgaaac tggcgacgaa ctgacgcgcc gagcccagtt
     2821 ccaggcgctg cacgatctcc aggcgtgtcg ccgccgtggc ggtggccgtc aaggcgatgc
     2881 gtggcacctg cggccagcgt tcggccagga tactgagccc caggtactcc gggcggaaat
     2941 cgtgccccca ttgcgagaca cagtgcgctt cgtcaatggc gaacaaggcg atgcgcccgc
     3001 gcgaaagcaa ttccatgcag cggtcggtca gcagacgctc gggcgccacg tacagcaggt
     3061 cgatgcgccc ggccaggaat tcctgctcga cttcgcgcgc ttcgcgccag tcttgcgtgg
     3121 aattgaggaa cgccgcccgc acaccctgtt caacgagcgc gctgacctga tcctgcatca
     3181 gtgcaatgag cggtgacacc accaccccca cgccatcgcg caccaatgcg gggatctggt
     3241 aacacagcga cttgccgccg cccgtgggca tcaggaccag tgcatcgccc ccatcgatca
     3301 gatgctcgat gatggtctgc tgttcacccc ggaacgactc gtatccgaat atccgccgca
     3361 ggacatccag cgcggttgag ggacggggag gcgttgacgg ggaggatgac ggcattgggg
     3421 ggaatagatg aacttggcga atggcaccga agacaagggg tcgaaccagt caaacggggc
     3481 actggcgcgg cgtcggcgaa agaggcattg taagcctgcg caccatggcg cccgaggcgc
     3541 gggccatgga atatccacac caatacccgc gcaaatggcg tatcccaccc atggcccagg
     3601 cgccaccccc ggggccgttc ttatgacagc gcggcccggg gcttggacag ttgatgatcc
     3661 agatgcgcct tcaccgtggg ccattcgctg gcaatgatgc tgtagacgca ggtatcacgc
     3721 aacgtaccgt tgggcgagat ctggtgttgg cgcagaaccc catcaagctt ggcgcccaga
     3781 cgctctatgc cggcacggct ttgatggttg aggaaatggg tgcgccactc caccgcgata
     3841 cagtccagtt cctcgaacgc atgcgtgagc agcatgagct tgcattcggt gttgatcccg
     3901 ctgcgctgaa cgcgccgcct a
//
LOCUS       DASTNK010000160         5514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_151490, whole genome shotgun sequence.
ACCESSION   DASTNK010000160 DASTNK010000000
VERSION     DASTNK010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151490"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="VFR20_11920"
     CDS             <1..1024
                     /locus_tag="VFR20_11920"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0231272.1"
                     /translation="PSVGVDAAKKLVDVQHVPAIVGALSSGVSAAVLTSVTAPGKVVL
                     ISPSSTSPTFTKLAKEGKTDGYWFRTTPSDALQGVAMAKVALDAGYKNVAVLYLNNAY
                     GQGLSAQFVNAFTKLGGKVTQDVVYNPAQASYRSEVNKALSPKPDALFLIGYPGDGTT
                     IAREWISAGGTQKFLFPDGLESQPFVNDVGPKYMTHVHGTAAGSVKTPSLATFQQEFQ
                     KKYGELPTQAYIPNAYDGAVIVGLAMEYAKSATGTAIRDSIRKVTDPNGTKIYAGAEQ
                     LKKAAKLIAEKKPIQYVGASGPLEFDANGDIAAPMVVWSVKGGKVENVGMVTVDQIDT
                     LRKKIK"
     gene            1107..1865
                     /locus_tag="VFR20_11925"
     CDS             1107..1865
                     /locus_tag="VFR20_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0231273.1"
                     /translation="MLEVKNLVREFGGLRAVDDVSFTLRPRSITGLVGPNGAGKTTLF
                     NTIAGFHRPTSGNISFLGKRIGGLPPHRIFREGLVRTFQIPRPFAAMTVLENAMLAPA
                     GQAGERFWNNWFSRATVRAQERACRDRAREILTFVGLEKLAGEYARNLSGGQQKLLEL
                     ARVLMADPKLILLDEPGAGVNPSLLVIIMDKLRELNERGITFLIIEHNMDLIMNLCDP
                     VLVMAQGRLILEGPPSVVRSDPTVLEAYLGGVTP"
     gene            1862..2596
                     /locus_tag="VFR20_11930"
     CDS             1862..2596
                     /locus_tag="VFR20_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0231274.1"
                     /translation="MSDAALSVTGLVAGYVPECDILRGIDLRVDEGEIVTVLGPNGAG
                     KSTLIKTIAGLVPVRGGNVRLFGDDLGGVAAHDMVRRGLAYVPQTNNVFVRLSIEENL
                     QLGAYIEQDRDAIREGVERMFALFPSLNNRRRKPAGTLSGGERQMVAVARALMAQPKV
                     LMLDEPSAGLSPKLVGVVFSKVREVRDMGVTILVVEQNAKAALAISDRGYILAEGKDR
                     INGQASELLHNPEVGELYLGIRRGLT"
     gene            2596..3486
                     /locus_tag="VFR20_11935"
     CDS             2596..3486
                     /locus_tag="VFR20_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0231275.1"
                     /translation="MLQYFVNGIILGATLALGAIGLTLTYNILRFANFAHGEFLTFGA
                     YFALVFASLFVSGATIGPLTFGWWFLLAIVVAAVLTAGLALLLDWLLFRLLRRREIEV
                     TLVIASFGASLLLRNVVIFVWGPQPEYYSRNIAIAMEIFPGMRMTADEIFVIVLTAVL
                     MVALHLFLTRTRLGKAMRAVSDNPALASITGIDVHGVIRWTWIVGGALAAVAGVLFGL
                     TVQIAPDMGFNLILPMFAAAILGGVGSIYGAVLGGLIIGLAESMSVPFIGAAYMPAVG
                     FGLMFLILLIYPKGILGDRS"
     gene            3483..4451
                     /locus_tag="VFR20_11940"
     CDS             3483..4451
                     /locus_tag="VFR20_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0231276.1"
                     /translation="MSGLGSYLVFFLIQALVFGVVCLGLNLQWGYTGLFNIGVSGFFL
                     VGAYAFAILCGPAYGTHLGGFGLPYGVGLAGAALAAAVIGFIVGIPTLRLHADYLAIV
                     TIGIGAIFQLIALNASFLTGGSQGATSIPRLLPDLFQSVLGRNLLMLAVMVVTVVVIY
                     AALEAIVRSPWGRVLKAIREDEEAASSLGKNAFSFRLQSFVIGSAIMGLGGALYASFI
                     GYVSPQDFMPILTFQVWTMLIVGGSGNNKGAILGAVLMWGVWTLSGSLAQAMLPIDLQ
                     VKGGAAQTILIGLVLMLVLIFRPPGIIGEEATVSREAHLTPDEPHH"
     gene            complement(4459..>5514)
                     /locus_tag="VFR20_11945"
     CDS             complement(4459..>5514)
                     /locus_tag="VFR20_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEU0231277.1"
                     /translation="TLAEDEAAQDKGDNVAGISHAMMTEHYALPGQLVVGTDSHTPHS
                     GALGCVAFGVGSTDMANAFITGAVRMTLPESLLIRLEGHPPAGITAKDIVLDLLARTE
                     IRAGLGVGKVFEFAGPVVDGLSVDERATLTNMTAELGGLTGIVAPDSRTVRFLLERRG
                     VEFVVEPWMRSDTGAHYAATLWVDCARLTPMVARPGDPGNGIALKDLPGPVRVDIAYG
                     GSCTAGKRADFDLYHEVLAWGLARGLRVADSVTLYLQYGTTAVRDYCIERGYDRVFQA
                     VGARILEPSCGACANCGPGSSTRADQVTVSAINRNFPGRSGPGEVWLASPPTIVASAL
                     AGELTSFEELKGRQIQA"
BASE COUNT          912 a         1734 c         1824 g         1044 t
ORIGIN      
        1 tccttcggtt ggcgtcgatg ccgccaagaa actggtggat gtgcaacatg ttccggctat
       61 tgtcggtgcg ctgtcgagcg gcgtcagcgc ggccgtgctg acctcggtca cggcgccagg
      121 caaggtcgtg ttgatttcac cgtcttccac gtcccccacc ttcaccaaac tcgctaagga
      181 aggcaaaacc gacggttact ggttccgcac cacgccgtcc gacgccttgc aaggggttgc
      241 gatggccaag gtggcgctgg atgccggata caagaatgtg gcggtcctgt atttgaacaa
      301 cgcctacggg caaggcctga gcgcgcagtt cgtcaacgcg ttcaccaaac tgggtggcaa
      361 ggtcacgcaa gacgtcgtct acaaccccgc ccaggcgtca taccggtccg aggtcaacaa
      421 ggcgctcagc cccaagcccg atgcgctgtt cctcatcggc taccccggtg acggcacgac
      481 catcgcgcgc gaatggattt ccgccggcgg cacacagaag ttcctgttcc ccgacgggct
      541 ggaatcacag ccgttcgtca acgacgtggg tccgaaatac atgacgcacg tgcatggcac
      601 ggccgccggc tcggtgaaaa cgccttcgct ggccaccttc cagcaggaat tccagaagaa
      661 atatggcgaa ctgcccacgc aggcctacat tcccaatgcc tacgacgggg cggtcatcgt
      721 cggcctggcc atggaatacg ccaagagcgc cacagggacg gccatccgcg acagcatccg
      781 caaagtcacc gaccccaacg gcaccaaaat ctacgccggg gcggagcaac tcaagaaggc
      841 ggccaagctg atcgccgaga aaaaacccat tcagtatgta ggggcatccg gcccgctcga
      901 gttcgatgcg aatggcgaca tcgccgcgcc catggtggtg tggtcggtca agggtggaaa
      961 agtcgagaac gtcggcatgg tcaccgtgga tcagatagac accttgcgca agaagatcaa
     1021 gtagtcgtgc cagccgcccg cgcgccgtgg tgcgtccgcg ggcggggcgg ccagcgtggg
     1081 tccacggtgt tcgaagggta ggtcgtatgc tggaagtcaa gaatctggtc agggagttcg
     1141 gcgggctgcg cgcggtcgac gatgtatcgt tcacgctgcg gccccggtcc atcaccgggc
     1201 tggtcggtcc caacggcgcc ggcaagacca cgctgttcaa taccattgcc gggtttcatc
     1261 ggcccacgtc cggcaatatt tcctttctgg gcaagcgcat cggtggtctg ccgccacacc
     1321 gcatcttccg ggaagggttg gtgcgcacct tccagatccc gcgtccgttc gctgccatga
     1381 ccgtgcttga aaacgccatg ctggcgccag ccggccaggc aggtgagcgc ttctggaaca
     1441 actggttcag ccgtgccacg gttcgcgcac aggaacgcgc ctgccgcgat cgggcacgcg
     1501 agatcctgac cttcgtgggc cttgaaaaac tggccggcga atacgcgcgc aacctgtcag
     1561 gggggcagca gaagctgctt gaactggccc gcgtgctcat ggccgaccca aaactcattc
     1621 tcctggacga gcctggcgcc ggcgtcaatc ccagcctgct ggtcatcatc atggacaagc
     1681 tgcgcgagct caacgagcgc ggcatcacgt ttctcatcat cgagcacaac atggatctca
     1741 tcatgaacct gtgcgacccg gtgttggtga tggcgcaggg gcggctcatc ctcgagggcc
     1801 caccgtcggt cgtgcgcagc gatcccacgg tgctcgaggc ctacctcgga ggggtcacgc
     1861 catgagcgac gccgcgttga gcgtgaccgg cctggtggcg ggctacgtgc cggaatgcga
     1921 cattctgcgc ggcatagacc tgcgggtcga tgagggcgag atcgtcaccg tcctgggacc
     1981 caatggggcg ggcaagtcga cgctcatcaa gaccatcgcc gggctcgtgc cggtgcgggg
     2041 cggcaacgtg cggttgttcg gcgatgacct ggggggcgtc gcggcgcacg acatggtgcg
     2101 ccgtggtctg gcctacgtgc cccagaccaa caacgtgttc gtgcggctca gcatcgagga
     2161 gaacctccaa ctgggggcgt acatagagca ggaccgggat gcgatccggg aaggggtgga
     2221 gcgcatgttc gcgttgttcc cctcgctgaa caaccggcgc cgcaaacccg cgggcacgct
     2281 atcgggcggc gagcggcaga tggttgcggt ggcacgcgcg ctgatggcgc agccgaaggt
     2341 gttgatgctg gatgagccct cggcggggtt gtctccgaag ctggtcggcg tggtgttctc
     2401 caaggtgcga gaggtgcgcg acatgggcgt cacgatactt gtcgtcgaac agaacgccaa
     2461 ggcggcgctg gccatctccg atcgcggcta catcctggcc gaggggaagg atcgcatcaa
     2521 tgggcaggca tccgaactgt tgcacaaccc cgaggtcggc gaactctacc ttggcatccg
     2581 gcgggggttg acataatgct ccagtacttc gtcaacggca tcatccttgg cgcgaccctg
     2641 gcgttgggcg ccatcggtct gaccctcacg tacaacatcc tgcgtttcgc caatttcgcg
     2701 catggcgagt tcctcacgtt cggcgcctat ttcgcgctgg tgttcgccag cctgttcgtc
     2761 agcggcgcca ccatcgggcc gctcacgttc ggctggtggt tcctgctggc catcgtggtg
     2821 gcggcggtcc tgacggctgg cctggcgttg ctgctcgact ggctgttgtt ccgcctgctg
     2881 cgccggcgcg aaatcgaggt cacgctggtc atcgcgtcgt tcggcgcatc gctgctgctg
     2941 cgcaacgtcg tcatcttcgt ctgggggccg cagcccgaat actattcgcg caacatcgcc
     3001 atcgccatgg agatctttcc gggcatgcgc atgacggccg atgaaatctt cgtcatcgtg
     3061 ctgacggctg tcctgatggt cgcgctgcac ctgttcctga cccgcacccg gctgggcaag
     3121 gcgatgcggg ctgtgtcgga caacccggcg ctcgccagca tcaccggcat cgatgtgcac
     3181 ggtgtcattc gctggacctg gatcgtgggc ggcgcgctgg ccgccgtggc gggcgtgctt
     3241 ttcggcctga ccgtgcagat cgcgccggac atgggtttca acctgatcct gcccatgttc
     3301 gccgccgcca ttctgggggg cgtgggcagc atctacggcg ccgtgctggg cgggttgatc
     3361 atcggcctgg cggagtccat gtcggtgccg ttcatcggcg cggcctacat gcccgccgtg
     3421 gggttcgggc tgatgttcct gatactgctg atctacccca agggcattct gggagaccga
     3481 tcatgagcgg cctgggttca tatttggtgt tcttcctgat tcaggcgctg gtcttcggcg
     3541 tggtctgcct ggggctgaac ctgcaatggg gctacaccgg cctcttcaat atcggggttt
     3601 ccggattctt cctggtgggt gcgtatgcct tcgcgattct atgtgggccc gcatacggca
     3661 cgcatctggg tgggttcgga ctgccgtacg gcgtggggct ggcgggcgcc gcgctggccg
     3721 ccgcggtcat tgggttcatt gtgggaatac ccactttgcg cctgcatgcc gattacctgg
     3781 ccatcgtcac cataggcatc ggcgccattt tccagttgat tgccctgaac gcgtcgttcc
     3841 tgaccggcgg cagccagggc gccaccagca tcccccggct gctgcccgat ctgttccagt
     3901 ccgtactggg acgcaacctg ctgatgctgg cggtgatggt ggtgacggtg gtggtcatct
     3961 acgccgcgct cgaggccatc gtgcgttcgc cctgggggcg tgtgctgaaa gccatccgcg
     4021 aagatgagga agccgcttcc tcgctcggga agaacgcttt cagcttccgg ttgcagtcgt
     4081 tcgtcatcgg atcggcgatc atggggctgg gtggtgcgct gtatgcgagc ttcatcggct
     4141 atgtcagtcc acaggatttc atgcccatcc tgacgttcca ggtgtggacc atgctgatcg
     4201 tcggcgggag cggcaacaac aaaggggcca ttctgggcgc cgtgctgatg tggggggttt
     4261 ggaccctcag cggtagcctg gcgcaggcga tgctgcccat cgaccttcag gtcaagggtg
     4321 gtgcggcgca gaccattctt atcgggttgg tgctcatgct ggtgttgatt ttccggccgc
     4381 ccggcatcat cggcgaagag gccaccgttt cgcgggaagc ccacctgacg ccggatgagc
     4441 cgcatcattg aggcgtgttc acgcctggat ctgccgcccc ttcaattcct cgaaagaagt
     4501 caactctccg gccagcgcgc tcgccacgat ggtgggcggg ctggcgagcc agacttcgcc
     4561 cgggcctgat cgacctggga agttgcggtt gatggcgctc accgtgacct gatcggcgcg
     4621 ggtcgacgac ccggggccac agttcgcgca cgcgccgcat gacggctcca ggatgcgggc
     4681 gcccacggcc tggaacacgc ggtcgtagcc ccgctcgatg caatagtcgc ggacggcggt
     4741 cgtgccgtat tgcaggtaca gggtgaccga gtccgccacg cgcaggccgc gcgcaagccc
     4801 ccatgccagc acctcatggt agagatcgaa gtcggcgcgt ttccctgcgg tgcatgatcc
     4861 gccgtaggcg atgtccacgc gcacgggccc gggaagatcc ttgagcgcga tgccgttgcc
     4921 cgggtcaccc gggcgggcca ccattggcgt caaccgggca cagtccaccc acagcgtcgc
     4981 cgcgtagtgg gcaccggtgt cgctgcgcat ccacggttcc acgacgaatt ccacgccgcg
     5041 ccgttccagc aggaagcgca cggtgcggga gtctggcgcc acgatgccgg tgaggccgcc
     5101 gagttccgcc gtcatgttgg tgagcgtcgc acgttcgtcc accgacaaac cgtcgacgac
     5161 aggccccgcg aattcgaaaa ccttgcccac gcccaggccg gcgcggatct ccgtccgcgc
     5221 cagaagatcc agcacaatgt ccttggcggt gatgcccgcc ggcgggtggc cttcgagccg
     5281 gatcaacaac gattccggca gggtcatgcg cacagcaccg gtgatgaacg cattggccat
     5341 gtcggtggag cccacaccga aggcgacgca gcccagggcg ccgctgtgtg gcgtgtgcga
     5401 gtccgtgccg acgacaagct ggccgggcag ggcgtagtgt tccgtcatca tggcgtgcga
     5461 gatgccggcc acgttgtcgc ctttgtcttg cgcggcctcg tcttcggcga gcgt
//
LOCUS       DASTNK010000161         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1262035, whole genome shotgun sequence.
ACCESSION   DASTNK010000161 DASTNK010000000
VERSION     DASTNK010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262035"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VFR20_11950"
     CDS             <1..529
                     /locus_tag="VFR20_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RHO alpha subunit C-terminal catalytic
                     domain-containing protein"
                     /protein_id="HEU0231278.1"
                     /translation="LGQFVTCDDLAWEFAEHYSLQRVGIHNALAQPGTHTYKTWHDRL
                     LNYRDGRTPEFGAVWVTYFPTHMIELYPHVVVLSTLHPVSPQETINVVEFYYPEEIAA
                     FEREFVLAQRAAYMETAVEDDEIAERMDAGRKALWRRGVNEAGPYQSPLEDGMLHFHE
                     WYRRMMVAATVPNGR"
     gene            complement(575..1441)
                     /locus_tag="VFR20_11955"
     CDS             complement(575..1441)
                     /locus_tag="VFR20_11955"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU0231279.1"
                     /translation="MFNLLISAAQLQDRLARPGCLVFDVRHSLADHQAGRRAYEQAHV
                     PGALYLDHEQQLCAPRTGHNGRHPLPDLADFAALMCFQGLTSDSQVVAYDDSGGMFAA
                     HLWWMLRWLGHPAVAVLDGGWGAWRAAGGSVESGSNSPSVSEARAVQSLGSTGQPAMP
                     TVDAQAVLDNIQSAVFAVIDARAGDRFRGESEPMDPVAGHIPGALNRPFQQNLRPDGR
                     FKDPGVLRTEFDAATKGLPASRIAHQCGSGITACHNLFAMELAGLRGSALYPGSWSEW
                     CSDPSRPTATGA"
     gene            complement(1502..2395)
                     /locus_tag="VFR20_11960"
     CDS             complement(1502..2395)
                     /locus_tag="VFR20_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0231280.1"
                     /translation="MQSLWMLLACAMFAAMGACVKVAFDYGASLPEIVLFRGIPSVLL
                     LLAWARASRRPMRPISWRLHIWRNVSGITSMWLGFYALAHLPLSTAISLNYTAPLFIA
                     GWMLVWGGSQRDPVRIVAVLLGFLGVIGVLRPSIEHDHWLAAALGLGAGALAAVAMMQ
                     IRALGRVGEPEWRTVLLFSCGVCLTSFGGFALEGLAPIGLPAVLALICLGVVGLFGQL
                     AMTRAFGLGSTLLTAALQYATIIFAALLGIILWGDHPDFIAWAGMALIIASGLLSVWR
                     TYSEDRVMRGATTVVQKAAGR"
BASE COUNT          530 a          831 c          724 g          338 t
ORIGIN      
        1 tctcgggcag ttcgtcacct gcgatgacct ggcctgggaa ttcgccgagc actacagcct
       61 gcagcgcgtg gggatccaca acgcgctggc gcaacccggg acccacacct acaagacctg
      121 gcacgaccgg ctgctcaatt accgagacgg ccggacaccc gagtttggcg ccgtctgggt
      181 cacgtatttc ccgactcaca tgatcgaact ttacccgcac gtcgtggtgc tatccacact
      241 gcatcctgtc agccctcagg aaaccatcaa cgtggtcgag ttctactacc ccgaggaaat
      301 cgcggccttc gagcgggaat tcgtgctggc ccaacgggcc gcctatatgg aaaccgccgt
      361 cgaggacgac gagatcgcgg agcgcatgga tgccggccgc aaagcgctct ggcggcgcgg
      421 cgtgaacgag gccggcccct accagtcccc actggaggac ggcatgctgc acttccacga
      481 gtggtatcgg cgcatgatgg tcgcagccac cgtaccgaac ggccgctgac ccatcggcca
      541 cccaagccgt ccgctggcaa tcaccgcctc gcagtcacgc tcctgtggcg gtgggccgcg
      601 aggggtcact gcaccactcg ctccacgagc cgggatacaa cgccgagccg cgcaggccgg
      661 ctagttccat ggcgaacaaa ttgtggcaag cggtgatgcc cgagccacac tgatgcgcga
      721 ttcgcgacgc gggcagacct tttgtggccg catcgaattc ggtccgcagc acaccagggt
      781 ctttgaaccg tccgtcagga cgcagattct gctgaaacgg ccgattcaag gctcccggaa
      841 tgtggcccgc gaccgggtcc atgggttcgg attcaccacg gaaacggtca cccgcgcgcg
      901 cgtcgatcac cgcgaaaacc gccgattgaa tattatccag gaccgcttgg gcatccacgg
      961 tgggcatggc gggttgacct gttgaaccga gactctggac ggcccgggcc tcagataccg
     1021 agggactgtt cgagcctgat tcgaccgatc cgcctgccgc cctccaggcc ccccaaccgc
     1081 cgtcgagcac cgccaccgcc ggatgcccca gccaacgcag catccaccac agatgggccg
     1141 cgaacatgcc gccggaatca tcataggcca cgacctgcga atcggacgtc aacccctgga
     1201 aacacatgag cgccgcgaaa tcggcgagat cgggcaacgg atggcgcccg ttatgcccgg
     1261 tgcgtggcgc gcacagttgc tgctcgtgat caagatacaa ggccccggga acgtgggcct
     1321 gctcataagc ccggcgtccc gcctgatggt cggccagact gtgccgcaca tcgaacacca
     1381 gacaacccgg gcgcgcgagc cggtcttgca actgggcggc gctgatcagc aaattgaaca
     1441 cggtgaatct cctgacgagc cagacggaag ccccggcccc tgaatggaac acgcccgtat
     1501 tctaccggcc agccgccttt tgcaccacgg tggtggcgcc ccgcatgacg cgatcttcac
     1561 tgtacgtgcg ccagacggac aacaggcccg aggcgatgat cagggccatg ccagcccacg
     1621 cgatgaagtc agggtggtcg ccccagagaa tgatgcccag caacgcggcg aagatgatcg
     1681 tggcgtattg caatgccgct gtcagcaggg tcgaacccag cccgaaggcg cgcgtcatgg
     1741 ccaattggcc gaacaaaccg acgacgccca gacagatcag cgcgagcacg gctggcagac
     1801 cgatcggcgc cagcccttca agagcgaacc ccccgaaact ggtcaggcac acgccgcagg
     1861 aaaaaagcag gaccgttcgc cattcgggct cacccacacg ccccaacgcc cggatctgca
     1921 tcatcgccac ggcagccagc gccccggcgc ccaggcccag cgcggcggcc agccaatgat
     1981 cgtgctcgat gctgggacgc agcacgccga tgacgcccaa aaaccccaac aacacagcca
     2041 caatacgcac cggatcgcgc tgcgaaccgc cccacaccag catccagccc gcaatgaaca
     2101 acggagccgt gtagttcaga ctgatggctg tggatagcgg cagatgcgcc agcgcgtaga
     2161 agcccagcca catggatgtg atacccgata cgttgcgcca gatatgcagg cgccaggaga
     2221 tcggcctcat gggacggcgc gatgcgcggg cccaggccag caggagcagc acagagggga
     2281 taccccggaa caggacgatc tcggggagcg acgcgccata gtcgaacgcc accttcacac
     2341 aggcacccat ggcagcgaac atggcacacg ccaacaacat ccacagagac tgcatgcgat
     2401 caaccggaat tcaggataaa agg
//
LOCUS       DASTNK010000162         7440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1448096, whole genome shotgun sequence.
ACCESSION   DASTNK010000162 DASTNK010000000
VERSION     DASTNK010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7440
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448096"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFR20_11965"
     CDS             complement(<1..279)
                     /locus_tag="VFR20_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEU0231281.1"
                     /translation="MDDTQLLRYARHILLDEIGIEGQERLLGARVLVVGAGGLGSPVA
                     AYLAGSGVGHIVLADDDIVDLGNLQRQLLHTTARVGWPKARSGRHMLAD"
     gene            complement(281..1804)
                     /locus_tag="VFR20_11970"
     CDS             complement(281..1804)
                     /locus_tag="VFR20_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU0231282.1"
                     /translation="MSTRRLGSFGLVFLGAVAGVLLSLGISAAAQRDGPLPLKELQQF
                     ANVFAAIKSSYVEPMSDKQLIDDAIKGLFLDLDPHSTYLDPQAYKEMQAITEGGFGGL
                     GIEIGSEDGMPKVIAPIEDTPAARAGIMAGDIITAINGKPTKGMTLSEAVNLMRGEPK
                     TSIELTIRRQGHDKPLDFKIVRDIIKIRSVRSKMLPGDIAYARIAQFQDGTATDLVKQ
                     LHALSKNKAPRAIILDLRNDPGGLLESAIGVASTFLKSGDLVVSTKGRIPSSNHQYFV
                     KPVGYVNDSGNMVSGDIKWARTVPMVVLINIGSASASEIVAGALQDHKRATVMGNRSF
                     GKGSVQSVLPLSEDTGIKLTTARYYTPSGRSIQITGVVPNITVDDTAQGNLFRLPREV
                     DLKHHLLNKAATAEDRSDVMSDKPKVEPKMFEFGGTDDYQLHEAVNYLDGRPVKRRDP
                     SLVLAPEAKGKTKPKAKAGDKTTSQSTESLEKKTLLNNDKPHVEHFRVTPNGVIRVH"
     gene            complement(1879..3357)
                     /locus_tag="VFR20_11975"
     CDS             complement(1879..3357)
                     /locus_tag="VFR20_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEU0231283.1"
                     /translation="MRGVVAGFVLSLGCVVAVQAAESPSLADRKAAATRQRQDLRERI
                     QAIQKDIDHQQAAQRGAADQLRASESAISDINRQLADLADQARSIERTQRSLDAEMGV
                     QRTLLARRRAELADQLRAQYADGLSPWTALLSGNDPQAIGRDLSYLGYVSRARAETVR
                     GIQKALGNLADLRRQSEAQGRRLAEVRKDTSQRKVDLQTSENQRQQVLSRIEAQLKAQ
                     RAQANHMARDEKHLGGLIQNLDKAIAAAAEAARRAEAERQAEAARKAEAARQARAARL
                     AREQAEQARRRAEAAQADQARRQVEAAAQAEKANQQAQAVRQRAQIEQAGKAQEQAQS
                     ARTETAREASSVRRAPERAPEGTAPRGGFHGLGRGLPYPVRGEVQGRFGASRPDGGGV
                     WRGIVLRAPEGTPVHVIAAGRVVYANWLSGFGNIMIVDHGGGYMSIYAYNQTLLKRVG
                     DIVQAGETIATVGSTGGQVESGLYFEIRRDGAPVNPLLWLKH"
     gene            complement(3384..4136)
                     /gene="gpmA"
                     /locus_tag="VFR20_11980"
     CDS             complement(3384..4136)
                     /gene="gpmA"
                     /locus_tag="VFR20_11980"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEU0231284.1"
                     /translation="MHKLVLMRHGESQWNLENRFTGWTDVDLTETGRQQAVNAGKLLR
                     EHGFEFDLAYSSVLTRAIRTLWITLDTLDAMYTPIGLSWRLNERHYGALQGLNKAETA
                     AKFGDEQVLIWRRAYAIAPEPLDVDDPRHPRFDRRYAKIAVEKLPATECLQDTVARVV
                     PFWNDSIAPALRAGRRVLVAAHGNSLRALIKHLDGVSDDDIVHVNVPTGQPLVYELND
                     ELRPIRHYYLGDPAEIEAAVAAVAAQGKAKHD"
     gene            4338..4754
                     /locus_tag="VFR20_11985"
     CDS             4338..4754
                     /locus_tag="VFR20_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU0231285.1"
                     /translation="MDFILSQNNLYIIIIALISGAMLLWPTISKGRGGASSVQVPEAI
                     QLANQKNAIFIDVRKPEEFKTGSIPQARNLPRTELEAKLDSLPKNKPIIVVCDQGRES
                     AQVAALLRKKGFADAVSLAGGLRSWTKDGLPLSKKA"
     gene            4789..5046
                     /gene="grxC"
                     /locus_tag="VFR20_11990"
     CDS             4789..5046
                     /gene="grxC"
                     /locus_tag="VFR20_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEU0231286.1"
                     /translation="MANVTMYCTGVCPYCIRAEMLLKQRGVTNIEKIRIDLEPAQRAT
                     MMERTGRRTVPQIYIDGRHIGGFDDLSALDRAQGLAPLLAA"
     gene            5080..5601
                     /gene="secB"
                     /locus_tag="VFR20_11995"
     CDS             5080..5601
                     /gene="secB"
                     /locus_tag="VFR20_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEU0231287.1"
                     /translation="MADQDQNSQNSAQEGQEPSFGLQRTYVKDLSLEMPNAPQIFLEQ
                     EGPNVEVSINVGGQRLADTVFECTVTGTITTRINDKVLYLVEATQAGIFEAANIPEEQ
                     LDPLLGIVCPTMLYPYLRANIADAINRTSLPPVHLAEVNFQNLYEQRLEQLAKEGQKE
                     DSGIVLPPGVTRQ"
     gene            5618..6676
                     /locus_tag="VFR20_12000"
     CDS             5618..6676
                     /locus_tag="VFR20_12000"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU0231288.1"
                     /translation="MNARTVPRPRVSVLGAGSWGTALAALACGHNDTLLWARDTELAR
                     AINATHANQRYLADIALPKALRCTGLFDEAVDHVLGADDGQGLIILGVPVAGLSDTCH
                     QLARTLTARKPRDITVIWTCKGFEPETGRLPHQIANAALPGGTLRTGVLSGPSFAREV
                     AQGLPVALTIASTEAAVTRLATSALHGQTARIYASADVIGVEVGGALKNIMAIACGIA
                     DGLGLGTNARAALITRGLAEMQRLGVALGGQAETFMGLTGLGDLVLTATGPLSRNRQA
                     GLAIGQGQDPRQVLASGTTIEGARCAGAALALGRAHHIDLPITHAVCRVLFDGMAPHQ
                     AVVGLLARQARTEAASPP"
     gene            6714..>7440
                     /locus_tag="VFR20_12005"
     CDS             6714..>7440
                     /locus_tag="VFR20_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0231289.1"
                     /translation="MKAFSRSRRIWHKTALGLALAAATFSAPSFAGTWPDKPLHMIVP
                     FPPGSSPDILARVIADPLGKVLGQHVIVENKPGAGGNIGTREATRAKPDGYTILMTIN
                     GPMVTAPRLYKKTLGYKPDQLAPITLIGTSPNVLIVPQNSPAKTVSEFVALAKAKPDT
                     LNFGTVGPGSSSHLCMAMLEHKAGIKLQQSPYSGFPEVVRSVVSGDIQASFMVPGIAM
                     PQIQAHKVRALAITSLKPSDILPG"
BASE COUNT         1324 a         2490 c         2246 g         1380 t
ORIGIN      
        1 atcggccagc atatgacggc ccgaacgcgc tttgggccac cccacgcgcg cggtggtgtg
       61 cagcaactga cgctgaagat tgcccagatc gacgatgtca tcgtcggcga gcacgatgtg
      121 gcccacgccc gaaccggcca ggtacgccgc gaccggggag cccaatccgc ccgcccccac
      181 caccaggacg cgcgcaccca gcaaccgctc ctgcccttct atgccgatct cgtcgagcag
      241 gatgtgccgc gcatagcgca gcagctgggt gtcatccacg tcagtgaacg cggatcacac
      301 cgttgggcgt gacgcggaaa tgctcgacgt gtggcttatc attgttcagt agcgtttttt
      361 tttccagtga ctcggtggac tgggacgttg tcttgtcacc ggccttggcc ttgggcttgg
      421 ttttgccttt tgcctcgggc gccaacacca gcgatggatc acggcgcttc acgggccgcc
      481 catcaagata gttgacggct tcgtgcaact ggtagtcgtc ggtgccaccg aactcgaaca
      541 tcttgggttc gaccttgggc ttgtcggaca tgacgtcgct gcggtcttcg gcggtggccg
      601 ccttgttgag cagatggtgt ttgagatcaa cctcgcgcgg cagacggaac aggttgccct
      661 gcgcggtgtc atctaccgtg atgttgggaa ccacgcccgt gatctggatg gagcgcccac
      721 tgggcgtgta atagcgcgcg gtcgtgagct tgatgccggt gtcttcgctg agcggcaaca
      781 cgctttgcac cgaccctttg ccgaagctgc gattacccat gacggtggcg cgcttgtgat
      841 cctgcagcgc accagccacg atctcggagg cggaggccga gccgatgttg atcagcacca
      901 ccatgggcac ggtacgggcc catttgatgt caccggacac catgttgccg gaatcgttga
      961 cataaccgac aggcttgaca aagtactggt gattggacga cgggatgcgg cctttggtgg
     1021 aaaccaccag atcacccgat ttcaggaacg tggaggcgac gccgatggcg ctttcgagca
     1081 ggccgcccgg gtcgttgcgc aagtccagga tgatggcgcg cggcgccttg ttcttgctga
     1141 gcgcgtgcaa ttgcttgacc aggtcggtgg cggtgccatc ctggaactgg gcgatgcgcg
     1201 cataggcgat atcaccgggc agcatcttgc tgcgcacgct gcgtatcttg atgatgtcgc
     1261 gaacgatctt gaagtcaagt ggtttgtcgt gcccctggcg gcggatggtg agctcgatgg
     1321 aggttttagg ctcgccccgc atgagattga cggcttccga cagcgtcatg cccttggtgg
     1381 gtttgccgtt gatggcggtg atgatgtcgc cagccatgat tccagcccgt gcggcggggg
     1441 tgtcttcgat gggggcgatg actttgggca tgccgtcttc actgccgatt tctatgccca
     1501 ggccaccgaa gccaccctcg gtgatggcct gcatttcctt gtaggcctgc gggtccagat
     1561 aggtggagtg ggggtcgaga tccaggaaca gccccttgat ggcgtcgtcg atgagctgct
     1621 tgtcggacat gggctcgaca taactgctct tgatggcggc aaagacgttg gcgaactgct
     1681 ggagctcttt cagtggcagc gggccgtcac gctgcgccgc ggcggaaata cccaggctga
     1741 gcagcacacc tgcgacagcc cccagaaaga ccaagccaaa actgcccaac ctgcgagtgc
     1801 tcatgcacgt tcccgaagag gtttcgatcc agggcgccgc gccagccggc cgtcccattt
     1861 tgaaagatag taccgaaatt aatgcttcaa ccaaagtagg ggattgaccg gtgccccgtc
     1921 gcgccgaatt tcaaagtata gtccggactc gacctgacca ccggtggatc cgacggtggc
     1981 aatggtttcg ccggcctgga caatgtctcc cactcgcttg agcagggtct ggttgtaggc
     2041 atagatgctc atgtagccgc cgccgtgatc gacaatcatg atattgccga aaccgctaag
     2101 ccagttggca taaaccacgc gtccggcggc gatcacatgc accggtgtgc cttcgggcgc
     2161 gcgcaacacg atgccacgcc acacgccacc gccgtcgggc cggctcgcgc caaaccgccc
     2221 ctgaacctcg ccacgcacgg gatatggcaa ccctctcccg agaccgtgga aaccgccccg
     2281 gggcgcggtg ccttcgggcg cgcgttccgg ggccctccgg acgcttgaag cctcccgcgc
     2341 tgtctcggtt cgcgcagact gggcctgttc ctgcgccttc ccggcttgct cgatctgggc
     2401 ccgctgacgc actgcctgag cctgctggtt tgctttctcg gcttgcgcgg ctgcttccac
     2461 ctgtcgcctt gcctgatcgg cttgcgccgc ctcggcccgc cgacgcgctt gctcggcctg
     2521 ctctcgtgcc agccgcgcgg cgcgcgcctg gcgggcggct tcggccttgc gcgccgcctc
     2581 ggcctggcgc tcggcttcgg ccctgcgcgc ggcttcagcg gccgccgcga tggccttgtc
     2641 gagattctgg atcaacccac ccaggtgctt ttcgtcacgc gccatgtggt tggcctgggc
     2701 gcgctgcgcc ttcaactgag cctcgatccg gctcagtacc tgttggcgtt gattctcact
     2761 ggtttgcagg tcgaccttgc gctggctggt gtcttttcgc acctcggcca acctgcgacc
     2821 ttgcgcttcg gactgcctgc gaaggtccgc caggttcccc agggctttct gaatgccgcg
     2881 gactgtctcg gcgcgcgcgc gggaaacata gcccaggtag ctcagatccc gtccgatggc
     2941 ttgcgggtcg ttcccggaca gcaatgccgt ccacggcgag agtccatcag cgtactgcgc
     3001 acgcaattga tcggcgagct cggcccgacg acgggccagc agcgtccgtt gcacacccat
     3061 ttccgcgtcg agagaccgct gtgtccgctc gatcgagcgc gcctggtccg ccaagtcggc
     3121 cagttgtcgg ttgatgtcgg aaatggcgga ttcggacgcc cgcagttgat ccgccgcacc
     3181 gcgttgcgcc gcctgttgat ggtcgatgtc tttctggatg gcctggatgc gctcccgcag
     3241 atcctggcgc tgacgcgtgg cagccgcctt gcggtcggcc agggacggcg actcggcggc
     3301 ctgcacggca acgacgcacc ccaacgacaa cacgaaaccg gccaccactc cgcgcataaa
     3361 tatcctttca gccctgtttc gcgtcagtca tgtttagcct tcccttgcgc ggcgacggcc
     3421 gccacagccg cctcgatttc ggccggatcc cccagatagt aatggcggat cgggcgcagt
     3481 tcatcgttca gttcatacac cagcggctgg cccgtgggaa cgttcacatg cacgatgtcg
     3541 tcgtccgaca caccatcaag gtgcttgatg agcgcgcgca ggctgtttcc atgcgcggcc
     3601 accagcacac ggcgcccggc ccgtaacgcc ggggcgatgg agtcgttcca gaacggcacc
     3661 acgcgcgcca cggtgtcctg gaggcattcc gtcgcgggca gcttctcgac cgcgatcttg
     3721 gcgtaacgcc ggtcgaaacg cgggtggcgc ggatcgtcga cgtcgagggg ctcgggggct
     3781 atggcgtaag ctcggcgcca gatcaacact tgttcgtcac cgaacttcgc agcggtctca
     3841 gccttgttca agccttgcaa cgcgccataa tggcgttcgt tcaggcgcca gctcaggccg
     3901 atgggggtat acatggcgtc cagcgtatcg agcgtgatcc acaaggtgcg gatggcgcgc
     3961 gtcagcacgg aagaatacgc cagatcgaac tcgaaaccgt gctcccggag cagctttccc
     4021 gcattcaccg cctgttgccg gcctgtttcc gtcaggtcga cgtcggtcca ccccgtgaac
     4081 cggttctcca ggttccactg gctttcgcca tggcgcatca gcactagttt atgcataata
     4141 gatagttacc caaaaatatg tcttctggct cattttataa tcggcggcgg cggaaatccg
     4201 gctgacccgg caattggcgc tatcggcacc atcggggaag ccgtcagtgg cccgccagcg
     4261 cccgccaccc acacccgtct gcgtggatgg cgggcgccgc cggaagccct ctcccaacag
     4321 ccagttctgg atacatcgtg gattttattc ttagtcaaaa caatctttac atcatcatca
     4381 tcgccttgat ttcgggtgcg atgctgctct ggccgaccat ctccaagggg cgcgggggcg
     4441 cgtcgtcggt acaggttcca gaggccatac agctagccaa ccagaagaac gccatcttca
     4501 tcgatgtgcg caaacccgag gaattcaaga ccggcagcat tccgcaggcc cgcaatctgc
     4561 cacgcaccga acttgaggct aaactggact ccctacccaa aaacaagccc attatcgtgg
     4621 tttgcgatca gggccgcgaa tcggcgcaag tcgccgcgct gctgcgcaag aaaggtttcg
     4681 ccgacgcggt cagcctggcg ggtggcctgc gcagttggac caaggacggc ctgccgttgt
     4741 ccaaaaaagc ctgatgcgcc gatctttcgt gccaaaaccg aggaacccat ggcaaacgta
     4801 accatgtact gcacgggtgt gtgcccgtat tgcattcgcg cggagatgct gctcaaacag
     4861 cgcggcgtca ccaatattga aaaaatccgc atcgacctcg agccggcgca gcgtgccacc
     4921 atgatggaac gcacaggccg gcgcaccgtt ccgcaaatct acatcgacgg ccggcacatc
     4981 ggtgggttcg atgatctgtc cgcgctcgat cgcgcccagg gccttgcgcc cttgcttgcc
     5041 gcctgacgcg gcttctcttt catcctctca ggaaatcaca tggccgacca agaccaaaac
     5101 tcccagaaca gcgcgcaaga gggccaggaa cccagcttcg gcctgcagcg cacctacgtc
     5161 aaggatctct ccctcgagat gccgaacgcc ccccagattt tcctggaaca ggaaggtccc
     5221 aacgtcgagg tctccatcaa cgttggcggg cagcgcctgg ccgacacggt cttcgaatgc
     5281 acggtcaccg gcaccatcac cacgcgcatc aatgacaagg tcctgtatct ggtcgaagcg
     5341 acgcaagccg gcatcttcga ggcggcgaac atcccggagg aacagctcga tccactgctt
     5401 ggcatcgtat gccccaccat gctctacccc tatctgcgcg ccaacatcgc cgacgccatc
     5461 aaccgcacct cgctgccgcc cgtccatttg gccgaagtga acttccagaa cctgtacgag
     5521 cagcgtctgg aacaactggc caaggaaggg cagaaggaag attccggcat cgtgctgccg
     5581 ccgggcgtga cccgtcaata acaaggcact cccagcgatg aacgcccgca ccgtcccgcg
     5641 cccgcgcgtt tcggtgctgg gcgccggaag ctggggcaca gcgctggccg cgctggcatg
     5701 cggccacaac gacaccctgc tatgggcgcg cgacacagag ctcgcccgcg ccatcaacgc
     5761 cacacacgcc aaccaacgtt acctcgccga catcgccctg cccaaggcgt tgcgctgcac
     5821 gggtctattc gacgaggccg tcgaccacgt cctcggggcg gacgacggcc aaggcctcat
     5881 catcctgggt gtgccggttg cgggcctgtc ggatacctgc catcaactgg ctcgaacgct
     5941 gacggcccga aaaccccgcg acatcaccgt catctggacc tgcaaaggct tcgagccgga
     6001 aacgggccgc ctgccgcacc agatcgcgaa cgcggcgctg ccaggcggca cactgcgcac
     6061 aggcgtattg tccggtccgt cgttcgctcg cgaggtggca caaggcctgc ccgtggcctt
     6121 gacgatcgcc agcaccgagg ccgcggtgac acgcctcgcc acgtccgccc tgcacggcca
     6181 gaccgcgcgc atctacgcca gcgccgacgt cattggtgtc gaggttggcg gtgccctcaa
     6241 gaacatcatg gccattgcct gcggcatcgc cgacggactg ggcctgggca ccaatgcccg
     6301 ggcggccctg atcacccgcg gcctggccga gatgcaacgc ctgggtgtcg cgctgggtgg
     6361 ccaggcggaa accttcatgg gcctgaccgg cctgggtgat ctggtcctga cggctaccgg
     6421 cccgctatcg cggaatcgtc aagccgggct ggccatcggg cagggccagg atccgcgcca
     6481 ggtattggcc agcggcacca ccatcgaggg ggcgcgctgc gcgggcgccg cgttggcgct
     6541 gggacgcgcg catcatattg atctccccat cacccatgcg gtctgccgcg tgctgttcga
     6601 cggcatggcg ccccatcagg cggtcgtggg gctgctggcg cgccaggcac gcaccgaagc
     6661 cgcctcacca ccttgacacg ctcttccatt ttcccttttg ccaaggatcg ctcatgaaag
     6721 cattttcccg ttcccgccgc atctggcaca agaccgccct gggcctggcc ctggccgcgg
     6781 ccactttcag cgcgccgtcg ttcgccggca cctggcccga caaacccctg cacatgatcg
     6841 tgccgtttcc cccgggatcg tctcccgaca ttctcgcgcg cgtcatcgcc gacccgcttg
     6901 gcaaagtcct gggccagcac gtcatcgtcg aaaacaaacc cggcgccggc ggcaacatcg
     6961 gcacgcgcga agccacacgc gccaaacccg atggatacac catcctgatg accatcaatg
     7021 gcccgatggt caccgcgccc aggctctaca agaaaacact gggctacaag cctgaccaac
     7081 tcgcccccat cacgctgatc ggcaccagcc ccaacgtgct catcgtgccg caaaactccc
     7141 ccgccaagac cgtgtcggaa ttcgtcgcgc tggccaaggc aaaacccgac acattgaact
     7201 tcgggaccgt cgggccggga agctcgtcgc atctgtgcat ggccatgctg gagcacaagg
     7261 ccggcatcaa gctgcaacag agtccgtact cgggattccc ggaagtcgtc cgatcggtcg
     7321 tctcgggcga catccaggcg agcttcatgg tgccgggcat cgccatgccc cagatccagg
     7381 cgcacaaggt gcgggccctg gctatcacca gcctcaagcc cagcgacatc ctgccgggca
//
LOCUS       DASTNK010000163         9983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1078657, whole genome shotgun sequence.
ACCESSION   DASTNK010000163 DASTNK010000000
VERSION     DASTNK010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9983
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078657"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFR20_12010"
     CDS             <1..405
                     /locus_tag="VFR20_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU0231290.1"
                     /translation="TEETGTQIDINDDGTITIASADLDRAREAERRIKELTADVEVGQ
                     EYEGPVLRLLDFGAIVQVLPGRDGLLHISEIANYRIANINDVLKVGQVVRIKVIEADD
                     KGRLRLSMKAIGGIEAQGGPQGPAAASPEAQA"
     gene            914..2281
                     /locus_tag="VFR20_12015"
     CDS             914..2281
                     /locus_tag="VFR20_12015"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU0231291.1"
                     /translation="MACATFCLALLGLVLRSPYALAPYWPANPVLLGAFILRPRLVRP
                     ACWVAVLAAFVATDFFIAGTPVLKSFLLNGINVVGIGVGYLVFRRIFGAKVDFGQPFS
                     MLHLCVVAVASAVAEAVLGGVFASIVSDQGELAGALGWFSEEFLSFITLLPVMLSAPA
                     FLHRSRRKWRRRSLREAARACLPALALVASLLLSLLIKGSGAIAFPVIALSYCALSYR
                     FFTTSLLVAFFTIWTVEFAVRGILPEYTGPADIHGLISLRLAMASIALAPLIIASVMA
                     AQARVLAEWRFMANSDSLTGVLNPRGFYREARALLRALHKSRQPVAVLMMDLDFFKQI
                     NDTYGHGVGDGALIKAATLLREELRDTDISGRVGGEEFAVLLALDTPHEVETVAERIR
                     ASIEACGVPVEGRAPLRMTVSIGASFSNYAPAELSTLMHAADRALYQAKHAGRNRVCV
                     WRGAL"
     gene            2476..3015
                     /locus_tag="VFR20_12020"
     CDS             2476..3015
                     /locus_tag="VFR20_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HEU0231292.1"
                     /translation="MKKILLSALVLSASLSATAFAAEYPIGKPVDQGGMEVAAVFLQP
                     VEMAPAGMMRPAKDSDIHLEADIHALAHNPNGFPEGAWMPYLVIHYALQKAGSDKTLS
                     GDLMPMVANDGPHYGDNVKLEGAGKYKLTLTIAPPTANKMNHFGRHIDKETGVAPWFK
                     TFTTSYEFVYAGTGKKGGY"
     gene            3077..3385
                     /locus_tag="VFR20_12025"
     CDS             3077..3385
                     /locus_tag="VFR20_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU0231293.1"
                     /translation="MMLGLAGGAHADELPTFTLTFQADGTFEPARLEVPAGRFKLILV
                     NNSKEPVEFESLPLRKEKVMGPGVKSFVVITISRPGEYPFFDDFHQNVKGTLVVVPKK
                     "
     gene            3402..4274
                     /locus_tag="VFR20_12030"
     CDS             3402..4274
                     /locus_tag="VFR20_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEU0231294.1"
                     /translation="MDQVAFIVWRESVEALLVIGILFTWLRASPEGRKGLPWLWAGVL
                     AGLGLAVLLALVLLGISSWLSSEGQEWFQAIMALVACGLVVQMVVWMRRHGRTLKKDL
                     EGGMQTQLGRANWWGLLVLAMIAVAREGSETVVFLYGTISAGDASGAAWRLALAGIGG
                     FAAALLTFWVLQLGGRLITWRRFFRLTEILLLLLASALLVGGLDHLISLGVLPTLMDP
                     VWDSSWLLSTSGGLGKILADFAGYRAYPALSELLIWLGYWACVFYLLNRAGRPVANQK
                     ATAPVKAQAGATQR"
     gene            4277..5689
                     /locus_tag="VFR20_12035"
     CDS             4277..5689
                     /locus_tag="VFR20_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEU0231295.1"
                     /translation="MTTLGLVASKYAGYLRDHAPLLRKLQWGIVCFYLFLLIVPAMLP
                     LPGRTASLFNNLTIFAQFAFWGVWWPFVLVSIPLFGRAWCGLFCPEGMLTEWASEHGR
                     GWAIPKWMRWGGWPFVAFALTTIYGQLVSVYQYPWAVLAVLGGSTAGAMVVGYLYGRN
                     KRVWCKYLCPVNGVFNLLAKLAPWHYKVDEDAWRHPVRRTASINCAPLVPLRHMKGAG
                     DCHMCGRCSDYRGAVTLTPRSPEAEIVTVAQGDAWQTALIVFGLMGLAVGAFLWSASP
                     WFVTLKQSAALWLINHDVTWPLAQNAPWFVLTHYPSVNDTFAWLDGAGILFFIVGSTV
                     AVGGPIYLALWLANRVAPMPRRPQVVPVESVGHDAVGRVGLHKLALGLIPAAGIGVFL
                     GLSATTLSLLKNEGVPLYWATGARFILLSLALLWSLRLVWRILGTRGSSPWRRWAGEG
                     IVMLGLTPFCVAWVLFFVVW"
     gene            5807..6280
                     /locus_tag="VFR20_12040"
     CDS             5807..6280
                     /locus_tag="VFR20_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU0231296.1"
                     /translation="MPDLITLNVNGKDQSVDAEPDSMLLYALRDNLKLHGPKFGCGLS
                     QCGACTVIMDGTAIRSCVMPLSAVAGHKITTLEGLGTIDKPHPLQQAFIDEQAVQCGY
                     CINGMIMTASALLKKNPHPTRDDIKQALDGNLCRCGTHMRIVRAVERVAKSGEPA"
     gene            6277..8616
                     /locus_tag="VFR20_12045"
     CDS             6277..8616
                     /locus_tag="VFR20_12045"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0231297.1"
                     /translation="MNTRHRDVIESSGLSDPGANRRAFLKASGGLVLGFSLLGPAAVM
                     AAERALDLDTSVAAMKSRGPFPAVDPAKLDAWIAIHPDNTATLFTGKVEQGNGAPNAL
                     LQIAADELDFPFDRFFLVMGTTTDTVDQGPSYGSMAVRYAGPQIQHAAAAGRQVLLSM
                     AGKHFGVPVDKLTASAGVITVTGASGKSITYGKLVGGKRLDVTIGASGKTFGMKVAPG
                     ARLKDPAKYTVIGTSVPRKDIPGKVTGEFTYMQDVKVPGMLHGRVVRPYGVGARLLSV
                     DETGLKDIPGFVRIVRDHDFLGVVAETEWGAIQAAQKLGSKLHRKGPDDGLAKWSDWN
                     GLAATDQVWDAVRKAPGKTQSVARHGDVDKALGVAAHTFKATYLTPFQTHGSIGPECA
                     IADVGKDRAIIWGGTQMPHQSQRDLAELLGFPIDNVEVRWVEAAGQYGRNGLEHVMAD
                     AAIMSRAVGRPVRVQWMRWDSHGWDPKEPPIVQELEGALDDHGNVTAWRHHMWVPTFS
                     DTRLLPSELIGKPVGTKNLGNAAIGYEYTFDNADVSSHNESRVGVITAWMRAPGQFET
                     TFAMESFIDELAATAKQDPLAFRLKYLKEPRTIGVLKAAAKLYGWQPRAAFSAGKQSG
                     RKAKGRGIAWVNRDDSRVATIMDVEVDQQTGEIRVVRVVVAHDNGLVISPDGMRNQIE
                     GNIVQSVSRALHEEVTFNQAHVTSRDWAGYPILRFHEIPDSIDIVLVNNNPAFMSTGG
                     GEPSTCPTAAVISNAVYDAVGVRLRQMPFRPERVKAAMA"
     gene            8779..8864
                     /locus_tag="VFR20_12050"
     tRNA            8779..8864
                     /locus_tag="VFR20_12050"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8813..8815,aa:Leu,seq:taa)
     gene            complement(8993..9607)
                     /locus_tag="VFR20_12055"
     CDS             complement(8993..9607)
                     /locus_tag="VFR20_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU0231298.1"
                     /translation="MRKIRVIEHISLDGVIQAPGGPNEDSAGFDHGGWTVPHGDPAVG
                     EAITVAHGTAFDLLLGRRTYDIWSGYWPTANSGPIADSLNAATKYVATHRPDSLEWGP
                     VEDLGSDIVEGIRRVKSTNGPDLITWGSSTLTPVLLEHGLADEVLLFVYPLLLGTGKR
                     FFAAGTPPCELTLASTKAASSGVIINTYRPTNRPLRTGSYDDPT"
     gene            complement(9722..9809)
                     /locus_tag="VFR20_12060"
     tRNA            complement(9722..9809)
                     /locus_tag="VFR20_12060"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9773..9775),aa:Ser,seq:tga)
BASE COUNT         1711 a         2962 c         3259 g         2051 t
ORIGIN      
        1 accgaggaaa ccggcaccca gatcgacatc aacgacgatg gcaccatcac catcgccagc
       61 gccgacctcg atcgcgcccg tgaagccgag cgccgcatca aggaactgac cgccgacgtc
      121 gaagtgggcc aggaatacga aggcccggtg ctgcgcttgc tggatttcgg cgccatcgtg
      181 caagtgctgc cgggtcgtga cggcttgctg cacatctcgg aaatcgccaa ctatcgcatc
      241 gccaacatca acgacgtgct caaggtgggc caggtcgtgc gcatcaaggt gatcgaggcc
      301 gacgacaagg gtcgtctgcg tctgtccatg aaggccatcg gtggcataga agcccagggc
      361 ggcccgcaag ggccggccgc ggcgtctccc gaagcccagg cctgaccacg gcagtgggca
      421 tcactgcccg ctgtctggtt ggcggccgcc tgtgccggtg cattgtccgg cgcggcggcc
      481 gttctgtttt ccgcaaccta tgtgcggtca ggccgctgag cactggattg ccttttggcg
      541 atggtgttct ctccgctgat ccccttcagg cccattcgta ataacccaga tgggcaggtg
      601 tatcccgtcg ccaagcagca gaccaatccg cgccggcgcc tgaatacgct ccagcgccag
      661 accgagccat tgcaaagagg gcaggcggca cgatctgtat cgaaaatagt caatatttga
      721 caacccctat ggctctttcc ctgaccgggc accataatgt cgggcgcaaa gggctatgga
      781 catgggaagt ggaatcaggg cggcatcgaa tggtggtcgg gacgacgttg ggtcagtcct
      841 aacgcttgcc gctcgtgttc gccacgcatg ggccagggcc gcctgccgct ggcctgtgct
      901 ggacatcttg ctgatggctt gtgcaacgtt ctgtctggcc ttgctcggcc tcgtgttgcg
      961 ttcaccctat gctctggcgc cgtattggcc ggccaatcct gtacttctgg gggcgttcat
     1021 cctgcgccca cggctggtgc gacccgcttg ctgggtcgcc gtgctggcgg cgttcgtggc
     1081 gaccgacttt ttcattgccg gcaccccggt gttgaagtcc ttccttctca atggcatcaa
     1141 tgtcgtgggc ataggcgtgg gctatctggt cttccgccgg atattcggtg ccaaggtgga
     1201 ttttggccaa ccgttctcga tgctgcattt gtgtgtggtg gctgtggcat ccgccgtggc
     1261 cgaagccgtg ctgggcggag tcttcgcatc catcgtgtcg gatcagggcg agttggcggg
     1321 cgcactgggt tggttctcgg aagaattcct gtccttcatt accttgctgc cagtgatgct
     1381 gagcgcgccc gcgttcctgc atcggagtcg gcgtaagtgg cgccgtcgaa gccttcgcga
     1441 ggcggcgcgt gcctgcttgc cggcgctggc actcgtcgca agcctgttgc tatcgctgtt
     1501 gatcaagggg agtggcgcca tcgcctttcc cgtgattgcg ttgtcctatt gtgcgctgtc
     1561 ttatcgcttt ttcacgacat cgctgctggt cgcgttcttc accatctgga ccgtggaatt
     1621 cgcggtgcgg gggattctgc ccgagtacac cgggccggcc gatatacacg gcttgatatc
     1681 gctgcgcctg gcgatggctt ccatcgcgct ggcgccgttg atcatcgcca gcgtcatggc
     1741 cgctcaggcc cgcgtgttgg cggagtggcg cttcatggcc aattcggaca gcctcaccgg
     1801 cgtgctcaat ccgcgtggct tttatcggga agcccgtgcc ttgctgcggg cgcttcataa
     1861 aagcaggcag cctgtggctg tgttgatgat ggatctggat ttcttcaagc aaatcaacga
     1921 tacctatggt catggggtgg gggatggtgc gttgatcaag gcagccactc tattgcgtga
     1981 agagttgcgc gataccgaca tcagtggccg agtcgggggg gaggaattcg ccgtattgct
     2041 ggcgcttgat acgccgcacg aggtcgaaac cgtggcagag cgcattcggg cgtcgattga
     2101 agcatgtggc gtgccggtgg aagggcgggc tccgctgcga atgacagtaa gcatcggcgc
     2161 gtcgttttcc aactatgctc cggccgaatt gtccacactg atgcacgccg ccgatcgcgc
     2221 gctctaccag gccaaacatg ccggtcgcaa ccgagtttgc gtgtggcgtg gagcattgta
     2281 atgagaatga atatcgtttg tctttatgag aaaaattgat tatcatttct catcgcgcca
     2341 gttttgacat tctagttgcg ttcgtcgttt tctaattgcc cctttgtcgt tgtcccgccc
     2401 ccgttggttg ggagtgtcca caggagggcg ttaggctggc ctggactttt gatcccgcat
     2461 caggaacaca cacgcatgaa aaagatactg ctgtccgcgc tcgtgttgag cgccagtctg
     2521 tcggccacgg ccttcgcggc ggaatacccc atcggcaaac cggttgacca gggtggcatg
     2581 gaagtggccg ctgtgttcct gcaacctgtc gaaatggctc ccgccggcat gatgcgcccc
     2641 gccaaggatt ccgacatcca cctcgaggcc gatatccacg cgctggcaca caaccccaat
     2701 ggcttcccgg aaggggcgtg gatgccttat ctggtcattc actacgcgtt gcagaaggcg
     2761 gggtccgaca agacgctgtc cggcgacctc atgcccatgg tggccaacga tgggccacat
     2821 tacggtgaca acgtcaagct cgaaggggcg ggcaagtaca agctcacact gacgatcgcg
     2881 ccgccaacgg ccaacaagat gaatcacttc ggccgccata tcgacaagga aaccggtgtg
     2941 gcgccgtggt tcaagacctt cacgacaagc tacgagttcg tgtatgcggg caccggcaag
     3001 aagggcgggt actgacatgc cgcgctttgt gaatacgaca tcgcgcgtcg tgggcgcatg
     3061 gctggcgggt ctgtgcatga tgctggggct ggccggcggc gcccacgccg acgaactccc
     3121 cacgttcacc ctcaccttcc aggccgacgg tacgttcgaa ccggcgcgcc tggaagtgcc
     3181 ggccgggcgc ttcaagctta ttctggtcaa caacagcaaa gagcctgtcg aattcgaaag
     3241 cctgcccttg cgcaaggaaa aagtcatggg tccgggcgtc aaatcgttcg tggtgatcac
     3301 catttcccgc ccgggtgaat acccgttctt cgatgacttt catcagaatg tgaagggcac
     3361 actggtggtc gtgcccaaga agtgaacgga atcgcataat catggatcaa gtggcattca
     3421 tcgtctggcg ggaaagtgtc gaagccttgc tggtgatcgg tatcctgttc acctggctgc
     3481 gggccagccc tgaagggcgc aagggcctgc cctggttgtg ggcgggcgtg ctcgccggcc
     3541 tggggctggc ggtgttgctg gccttggtgc tgttgggcat atcgtcctgg ctgtccagcg
     3601 aggggcagga atggtttcag gccatcatgg cgttggtggc gtgcggcctg gtcgtgcaaa
     3661 tggtcgtgtg gatgcgtcgc cacggcagaa ccttgaagaa ggatctcgaa ggcggcatgc
     3721 agacccagct ggggcgggcg aactggtggg ggcttctggt gctggccatg atcgcggtgg
     3781 cgcgcgaagg cagtgaaacc gtcgtgttcc tgtacggtac catttccgcc ggggacgctt
     3841 ccggcgcggc gtggcggctg gctctggcgg gtatcggcgg tttcgcggcg gcgttgctca
     3901 cgttctgggt gctccaactg ggcggcaggc tcatcacgtg gcggcggttt ttcaggctta
     3961 ccgagatcct gctgttgctg ttggccagcg cgttgttggt tggcgggctc gatcacctga
     4021 tctcgctggg tgttctgcca accctaatgg atccggtgtg ggacagctcc tggttgttga
     4081 gcacgtccgg cggcctgggc aagattctgg ctgatttcgc cgggtatcgc gcttatccgg
     4141 ccttgtcgga attgctgatc tggctgggtt actgggcctg cgtgttctac ctgctgaatc
     4201 gagccggaag gccagtcgcc aatcagaagg ccaccgcgcc ggtgaaggca caggcgggcg
     4261 cgacacagcg ataatcatga cgacgctcgg cctggtcgcc tcgaagtacg cagggtacct
     4321 gcgcgatcat gccccgctgt tgcgaaagct gcagtggggt attgtctgct tctacttgtt
     4381 tctgctgatc gtgccggcga tgctgccgtt gcccggccgc acggcgtcat tgttcaataa
     4441 cctgaccatc ttcgcccagt tcgcgttctg gggcgtgtgg tggccgttcg tgctggtgtc
     4501 cattcccttg ttcggacgcg cctggtgcgg cctgttctgt ccggaaggga tgctcaccga
     4561 gtgggccagc gagcacggcc gagggtgggc cattcccaag tggatgcgct ggggcggctg
     4621 gcctttcgtg gctttcgcgt tgaccaccat ctatggtcaa ttggtcagtg tgtaccagta
     4681 cccctgggcg gtgctggccg tgctgggcgg atccacggcc ggggccatgg tggtcggata
     4741 cctgtatggc cgaaacaagc gcgtgtggtg caagtatctc tgtccagtga acggcgtgtt
     4801 caacctgctg gccaagttgg ctccgtggca ctacaaggtg gatgaagacg cctggcgcca
     4861 ccccgtccgg cgcaccgcgt ccatcaattg cgcgccgctg gtgccgctac ggcacatgaa
     4921 gggagcgggt gattgtcaca tgtgtgggcg ttgcagcgat taccgtggcg ccgtcacgct
     4981 gaccccgcga tcacccgaag ccgagatcgt cacggtggcg cagggcgacg catggcagac
     5041 ggcgctgatc gtgttcggcc tgatggggct ggcggttggc gccttcctct ggagcgccag
     5101 cccctggttc gtgacactga aacagtccgc cgcgttgtgg ctgatcaacc atgacgtgac
     5161 ctggccgttg gcgcagaacg cgccctggtt cgtgctgacc cattaccctt ccgtcaatga
     5221 cacgttcgca tggttggatg gtgcgggcat cctgttcttc attgtcggtt caacagtcgc
     5281 ggtgggcggg ccgatctacc tggcactgtg gctggccaac cgcgtggctc cgatgccgcg
     5341 gcggccgcaa gtcgtgcccg tggagtccgt gggacatgat gccgtcggtc gggtcgggct
     5401 gcataagctg gccctgggcc tgattccagc ggcgggtatc ggggtctttc tgggcctttc
     5461 agccacgaca ttgtccctgc tcaagaacga gggtgtgccg ctgtattggg ccaccggcgc
     5521 ccgcttcatc ctgttgtcgc tggccctgct gtggagtctg aggcttgtgt ggcgcattct
     5581 cggaacacgt ggctcatcgc cttggcgccg gtgggcgggg gagggcatcg tcatgctggg
     5641 cctgacacca ttctgcgtgg cgtgggtact gttctttgtc gtgtggtagc tgtcgcatcc
     5701 tccatccatg atgttcgctg tcttgccgcg ggtttagaat catgggcaag ggatcgtaaa
     5761 ccgcccatca gcggtaatgc gcggctcaca tcaaggagat tgactgatgc ccgacctcat
     5821 cacgctcaac gtcaacggta aagaccagtc ggttgacgcg gaaccagaca gcatgctgct
     5881 gtacgccttg cgcgacaacc tcaagcttca cggaccgaaa ttcggctgcg gcctgtccca
     5941 gtgcggggcc tgcaccgtca tcatggatgg caccgccata cggtcctgtg tcatgccgct
     6001 gtcggcagtg gcgggccata agatcaccac gctggaaggc ttgggaacca tcgacaaacc
     6061 gcacccgctc caacaggcct ttatcgatga gcaggccgtg cagtgcggtt actgcatcaa
     6121 cggcatgatc atgacagcca gcgcactgct caagaagaac ccgcatccca cgcgcgacga
     6181 catcaagcag gcgctggacg gtaacctgtg ccgctgcggt acgcacatgc gcatcgtccg
     6241 cgccgtggaa cgagttgcca agtcgggaga accggcatga atacgcgaca tcgtgatgtg
     6301 atcgaatcct ccgggctttc cgaccccggc gccaatcgcc gcgcgttcct caaggcctcg
     6361 ggagggctgg tgctgggctt cagcctgctc gggccggctg cggtgatggc ggccgagcgg
     6421 gcgttggatc tggatacgag tgtggccgct atgaagagca ggggcccgtt cccggcggtg
     6481 gatccggcca agctggacgc gtggatcgcc atccatccag acaacaccgc cacgctgttc
     6541 acgggcaagg tggagcaggg caacggtgcg ccgaatgctc tgctgcagat tgccgccgac
     6601 gaactggact tcccgttcga tcgctttttc ctcgtcatgg gcacgacgac tgacacggtc
     6661 gaccaagggc cttcttacgg cagcatggcg gtccgatatg ccgggccgca gatccagcac
     6721 gctgcggcgg cgggccgcca ggtgctgctg tcgatggctg gcaagcactt cggcgtgccg
     6781 gtggacaaac tgacggcgag cgcgggtgtc atcaccgtca cgggcgcgtc gggcaagtcc
     6841 attacttacg gaaaactggt gggcggcaag cgcctcgacg tgaccatcgg cgccagcggc
     6901 aagactttcg gcatgaaggt ggcgcccggg gccaggctca aggatcccgc caagtacacc
     6961 gtgatcggca cctcggtgcc gcgcaaggac attcccggca aggtgacggg ggaattcacg
     7021 tatatgcagg acgtgaaagt gcccggcatg ctgcacggcc gcgtcgtgcg cccgtacggc
     7081 gtcggcgcgc ggcttttgtc ggtcgatgaa accggcctca aggacattcc cggcttcgtc
     7141 cgaatcgtgc gcgaccatga cttccttggc gtggtggcgg aaaccgaatg gggggcgatc
     7201 caggcggcgc agaagctggg ttccaaactg catcgcaagg ggccggacga tggtctggcc
     7261 aagtggtcgg actggaatgg cctggcggcg acggaccagg tctgggacgc ggtgcgcaag
     7321 gcgcccggaa agacgcagtc cgtggccagg cacggtgatg tcgacaaggc gctcggcgtc
     7381 gcggcgcaca cgttcaaggc cacgtatctc acgccgttcc agacacatgg ttcgatcggc
     7441 ccggagtgcg ccatcgccga cgtgggcaaa gaccgagcca tcatctgggg cggcacgcag
     7501 atgccgcatc agtcgcagcg tgacttggct gagttgctgg gttttccgat cgacaacgtt
     7561 gaagttcgct gggttgaagc cgcaggtcag tacgggcgca atgggttgga gcacgtgatg
     7621 gcggacgccg ccatcatgtc gcgcgccgtg ggccggccgg tgcgtgtgca gtggatgcgg
     7681 tgggattcgc acggctggga cccgaaggaa ccgccaattg tccaggaact cgagggggcg
     7741 cttgacgatc acggcaatgt cacggcgtgg cgtcatcaca tgtgggtccc gacgttctct
     7801 gatacgcgtc tccttccttc ggagttgatc ggcaaaccgg tcggcacgaa aaatctcggc
     7861 aacgccgcga ttggctacga gtacacgttc gacaacgcgg atgtgtcgtc ccacaacgag
     7921 agtcgcgtgg gcgtgatcac ggcctggatg cgtgcgccgg gccagttcga gaccaccttc
     7981 gccatggagt ccttcatcga tgaactggcg gcgacggcaa agcaggatcc cctggcgttc
     8041 cggctcaagt atctcaagga accacgcacc atcggggtcc tgaaggccgc cgccaagctc
     8101 tacgggtggc agccgcgcgc cgcgttcagc gccggcaagc agagcggacg caaagccaag
     8161 ggccgcggca tcgcgtgggt caatcgcgac gacagccgcg tggcgaccat catggatgtc
     8221 gaagtcgacc agcagacggg cgagatccgc gtcgtgcgcg tcgtcgtcgc gcacgacaat
     8281 ggcctggtga tcagcccgga cggcatgcgc aaccagatcg agggcaacat tgtccagtcg
     8341 gtcagtcgcg cgctgcacga ggaagtgacg ttcaaccagg cgcacgtcac cagtcgtgac
     8401 tgggcgggct atccgatcct gcggttccac gagatcccgg acagcatcga tatcgtgttg
     8461 gtgaacaaca acccggcgtt catgtcgacg ggcggcggcg aaccttcaac ctgccccaca
     8521 gcggcggtca tcagcaatgc cgtctacgac gcagtggggg tgcgcctgcg ccagatgccg
     8581 ttccggcccg agcgcgtaaa ggcggccatg gcttgatgac tggtggcgtt gtgtgtgccc
     8641 cattggggcg caccatcgcc tcgacccagt gaggccacac accccatgcg tgtgcgagag
     8701 ggggcgtgtt ggcgctgctt tgtggtggtt tgaactgtgt attacgcacg aattacgcgc
     8761 gtgtatactt caggcctcgc tcgggtggcg gaattggtag acgcagcaga cttaaaattt
     8821 gccggccata ggccatacgg gttcgattcc cgtcccgagc accaatcaat tcggctgacc
     8881 ttatgtcagg ccatcccgcg ccaaatcccg ccttgcctgc ttcgccgtgg tcgaaccaca
     8941 ggcagggaat aaggttggcc atatctgtct cctgcgggtt ggaagcccat cttcacgtcg
     9001 gatcgtcata ggatccagtg cgcaaaggcc ggttggtggg tcggtaggtg ttgatgatga
     9061 cacccgaact cgccgctttc gtgcttgcga gcgtgagttc gcatggcgga gtgcccgccg
     9121 caaagaaacg tttgcccgtg cccagcagaa gcggatagac gaacagcagg acttcgtcgg
     9181 cgagcccatg ttcgagcagc actggcgtca gtgtcgagct gccccaagtg atcaggtccg
     9241 gtccatttgt tgacttgacg cggcgaatac cctcgacgat atccgatcca agatcttcca
     9301 ccggccccca ttcaaggctg tccggcctgt gagtggccac gtatttcgtc gccgcgttca
     9361 ggctgtccgc gatcggaccg ctgttggctg ttggccaata gccgctccag atgtcgtagg
     9421 ttcggcggcc cagcagcaga tcgaaggccg tgccgtgcgc cacggtgatg gcttccccca
     9481 ctgcggggtc gccatgcggc accgtccagc cgccatggtc gaagccggca ctgtcttcgt
     9541 tcggtccgcc gggggcctgg atcacgccgt ccagagaaat atgctcgatg accctgatct
     9601 tcctcatggt gcctccgtgc ttattttggt tagaacatga atgtaaacac ccccgtaggc
     9661 ggcatatacg cgccccgcgg ctgtttcatg cttcgtcaag agcctgcgac accccgcttt
     9721 ttggcggaag gggtgggatt cgaacccacg aacggttgcc cgttgccggt tttcaagacc
     9781 ggtgcaatcg accactctgc cacccttcca gcggctggtt ccggatacgc ccgctttcat
     9841 gtgggccagg ctattcgtct gtgcggcgcg gaccctgtta ttctttttgt tttcgcggcc
     9901 agacgacgcc cgaacccgga agcccgaaag tgtaccccat actacgcttt caggtgaacg
     9961 ggcggcgcgc cggcgggtca ccc
//
LOCUS       DASTNK010000164        18093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1633978, whole genome shotgun sequence.
ACCESSION   DASTNK010000164 DASTNK010000000
VERSION     DASTNK010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 18093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18093
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633978"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="VFR20_12065"
     CDS             complement(<1..1247)
                     /locus_tag="VFR20_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEU0231299.1"
                     /translation="MEDNLRKAALEYHEHGRPGKISVTPTKQLSNQRDLALAYTPGVA
                     AACEEIVADPKNAFRYTARGNLVGVITNGTAVLGLGNIGPLASKPVMEGKAVLFKKFA
                     GLDVFDIEINETDPDKLVDIIAGLEPTFGGINLEDIKAPECFTVERKLRERMGIPVFH
                     DDQHGTAICVSSAFISGLRVAGKAIDQVKVVVSGAGAAALGCLDLMVDLGLPLKNIWV
                     TDIEGVVYKGRTALMDPDKERFAQETGARKLADVIDGADVFLGLSAGGVLKPEMVAKM
                     AERPIILALANPTPEILPEEARAVRDDVIMATGRSDYPNQVNNVLCFPYIFRGALDVG
                     ATTITRGMEKAAVEAISRLAQEEQNDVVAAAYGTYGVSFGPEYLIPKPFDPRLIVRIA
                     PAVAKAAMDDGVATRPIADMEAYV"
     gene            complement(1416..2342)
                     /locus_tag="VFR20_12070"
     CDS             complement(1416..2342)
                     /locus_tag="VFR20_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231300.1"
                     /translation="MPSNSRRAFLMGRRTLRTPWEAFCQHMNRAMEGTFYDFGEQNGV
                     GSARLTPAHSSDVQRARELCVEHGVTMALDGVQHASALDDFPVLWIDPGRDMAECKRI
                     ADDSPYWFVQPGCMLGELEAVGLRQFRDLPAHLTVAAWLADRGLCDWRTGETYKSGLQ
                     HASVMLADGTRATLGPFGENSHQPLEGLTLQRLVPALFEQSTSQAAQTCRGQEFWPAR
                     YRLDALLPVPGQTVNLAHLLLGHGGDLGWVDWVVLDERVDNEIEASYFPRFSTNHEAH
                     DDDTWLAASDADSQVKYVFDPSNLFPFPGQNL"
     gene            2436..3596
                     /locus_tag="VFR20_12075"
     CDS             2436..3596
                     /locus_tag="VFR20_12075"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU0231301.1"
                     /translation="MPRLAQRTDRVMPFYAVELFKQAGALNAQGRDIISLGIGEPDFT
                     APAQVVETLRRAAEAGLSGYTAPAGLATLREAIAHYYGEHFSAPVDPDRVIVTSGASG
                     ALLLASMALINPGDEVLMPDPSYPANQNFITSAGGVPRLIPCQAEQNFQLSAADIRAQ
                     WTPATRGVLIASPSNPTGTTIAREDLKALVAEVRARDGFVIMDEIYLGLYYGDKPQSA
                     LTLDDDIVIINSFSKYFHMTGWRLGWLIAPPSMIPAVEKLAASLAICAPTLAQHAALT
                     CFEPDVMRVYEHRRQSFQQRRDYLLPEFERLGLHVPARPDGAFYIYADIRNFSQDSDD
                     FSHRLLHEAGVAAVPGRDFGEAHARHTMRFSYATGLDRLQEAVERMERFLER"
     gene            complement(3686..4666)
                     /locus_tag="VFR20_12080"
     CDS             complement(3686..4666)
                     /locus_tag="VFR20_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU0231302.1"
                     /translation="MGATRSENPLGLMVRSIALPLGELVHVAAVYLGIAALVLLALCL
                     TVPSVREQARQVHSALLAALQPDSIQNDESGTNDLAQYEMLLQEANNEAQPPQASGQE
                     GLPPTGATTNTGATGATATTGAAAGTSPQAEAESAGGKPGATGAVKSAPALHQSFVRE
                     LKASVHGLEVKGISHAQAQALRSYIARKYKIASSVAGVLVKTAFQVGRQKHLDPQLLL
                     AVIAIESGYNPYAESHVGAQGLMQVMTQVHIDKFDRFGEGAAAALNPIANIRVGAQIL
                     DECIKRRGSVSGGLACYVGATGPGDGGYGARVMAEQRRLALASGIPISNK"
     gene            4780..6324
                     /gene="ubiD"
                     /locus_tag="VFR20_12085"
     CDS             4780..6324
                     /gene="ubiD"
                     /locus_tag="VFR20_12085"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEU0231303.1"
                     /translation="MKYRDLRDFIAQLERAGELKRVAARVDTDLEITEICDRVLRRAG
                     PALLFEHPVHQGADAAMPVLANLFGTPRRVAWGMGADDVGALRETGELLAALREPEPP
                     KGFRDALAKVSMLKAALWDMNPRTVKNPPCQDIVLEGADVDLDRIPLQRCWPGDVAPL
                     LTWGLVVTRGPNARRQNLGIYRQQRLGRNKLIMRWLSHRGGALDFRDHMLAHPGTPFP
                     VAVALGADPATILGAVTPVPDTLSEYQFAGLLRGSRTEVAKTLGSELDVPAFAEIVLE
                     GHLLPTSDPRAMRPDVPEGVSPPPDNGYEMALEGPYGDHTGYYNEQDWFPVFTIDRIT
                     MRRDPIYHSTYTGKPPDEPAVLGVALNEVFVPLLRRQLPEIVDFYLPPEGCSYRLAVV
                     SIRKQYAGHAKRVMFGLWSILRQFMYTKFIVVVDHDIDPRDWKEVVWAMTTRMDPVRD
                     AVLVENTPIDYLDFASPVSGLGGKMGLDATNKWAGETSRQWGIPIQMDRAVKDKVDAL
                     WDQLGL"
     gene            complement(6334..6681)
                     /locus_tag="VFR20_12090"
     CDS             complement(6334..6681)
                     /locus_tag="VFR20_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231304.1"
                     /translation="MKGVSARNERVMRIELARARAAVERERLKRDACDAVQELSPGRI
                     VSRFLSPLGRGHGPLAWLRNGAALARRYPLLVSAVSAAFSGAGGRRRRWWRLGAALLA
                     GIEAARLFSRPRD"
     gene            complement(6678..7100)
                     /locus_tag="VFR20_12095"
     CDS             complement(6678..7100)
                     /locus_tag="VFR20_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEU0231305.1"
                     /translation="MALSRAVGDLAGTLLSMARTRLELFALEAGEQKARLIGLLALLC
                     AAAFFLALAVLVLSVTVALYFWPTPYRYMALWLLGLLYLVLGIAFAWTVRVRLRGDPL
                     PFSATLDELRRDAALFGRLREPGAAHADGRDGAPENSP"
     gene            complement(7146..7466)
                     /locus_tag="VFR20_12100"
     CDS             complement(7146..7466)
                     /locus_tag="VFR20_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HEU0231306.1"
                     /translation="MSAKKPDAKATEEDFIESLKASLDDAEQLLRDAASTTGDKAAEL
                     RERAMTSLRHTRETLYDTQDALMERGRRAARATDDYVHDNPWQAIGMASVAGLLVGML
                     ISRR"
     gene            complement(7638..8276)
                     /gene="upp"
                     /locus_tag="VFR20_12105"
     CDS             complement(7638..8276)
                     /gene="upp"
                     /locus_tag="VFR20_12105"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU0231307.1"
                     /translation="MPIHEIRHPLIRHKLGLMRRGDLSTKNFREMAQEIAALLTYEAS
                     KDLPLEPHVIQGWSGEVTVEKLAGKKVTVVPILRAGIGMLDGVLTLIPGAKVSVVGIA
                     RNEETLQAEPYLEKLVGGLDQRLALIVDPMLATGGSMVATIDMLKRAGSVNIRALVLV
                     AAPEGIAAVEAAHPDVHVYTASIDSHLNKNGYIIPGLGDAGDRIFGTRQKEA"
     gene            complement(8300..9760)
                     /gene="dacB"
                     /locus_tag="VFR20_12110"
     CDS             complement(8300..9760)
                     /gene="dacB"
                     /locus_tag="VFR20_12110"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEU0231308.1"
                     /translation="MVRSGNSTSFFRAARRDWLAFLAGVALSLGSAGAMAQALPPQLQ
                     KAWQATGLPQSALSLVVQEVGGSRMVSVNPQTSRNPASVMKMVTTWTGLSGLGPDYQW
                     RTTLLGRDGARVDAQGTLRGPLYVKAGGDPFLTIQDLWNLLRELRLRGIKNLNEIVVD
                     RSLFGNVATDPGDFDDSPYRPYNASPDAMMVSLGAVRLLFEPDAAARKWVPIIDPPLR
                     GLRVEGNVKWSNARCPGSPTVGVTETASAQGVVIHVDGTAAGSCGEFSVYRLAMSQPD
                     YFDAVFRMLWLDLGGTLAKGFRAGRVPPSAQPIVWHDSVSLSDTIRAINKHSNNVMAR
                     TLLLTLGAEKNGPGATPASGANAALAILASQRVDTQGWHIVNGAGLARGSRLTAGGLV
                     SMLDTAWRSSLMPEFVSSLSISGMDGTMKHRLRSDGTRGAAHLKTGTLRNACSLAGYV
                     LGKSGKRYIVVSFVNDDRAGAARPFDDAVIAWLADS"
     gene            9969..11006
                     /gene="queA"
                     /locus_tag="VFR20_12115"
     CDS             9969..11006
                     /gene="queA"
                     /locus_tag="VFR20_12115"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU0231309.1"
                     /translation="MNTTLSLSQFDYHLPQELIAQTPADRRTGSRLLHLDGHGALHDR
                     QFTDLPDLLRPNDLLVFNDTRVIKARLMGHKETGGKIEVLVERITSPRTALAHIRSSK
                     SPRAGSRVVLADAFTATVTGRQGELFELDFPERVLDLLERHGATPLPPYITHPADAQD
                     EDRYQTVYARAPGAVAAPTAGLHFDLPLLEEIGRRGVGKAFVTLHVGAGTFQPVRVDR
                     IDQHVMHSEHYVVPAEVLEQIAATRANGGRIIAVGTTSVRALESVDQDAAPDGPAEGD
                     TRLFITPGYPFKRVDALITNFHLPQSTLLMLVSALAGMEPIRRAYAHAVDARYRFFSY
                     GDAMFIESSAS"
     gene            11003..12136
                     /gene="tgt"
                     /locus_tag="VFR20_12120"
     CDS             11003..12136
                     /gene="tgt"
                     /locus_tag="VFR20_12120"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU0231310.1"
                     /translation="MTGLDFTLLATDGAARRGRLVLNHGVVQTPIFMPVGTYGSVKAM
                     MPHELDEIGAQIVLGNTFHLWLRPGTDIIDRHDGLHGFMRWPRPILTDSGGFQVFSLG
                     GLRKITEEGVRFASPIDGARLFLSPEESMRIQRSLNSDIVMVLDECTPYSIDGRPATL
                     DEAARSMRMSLRWARRSKEAFNRLENPNALFGIVQGGMFEALRDESLAGLTDIGFHGY
                     AIGGLSVGEPKEEMLRVLSHVAPRLPAQAPRYLMGVGTPEDLVDGVASGVDMFDCVMP
                     TRNARNGWLFTRFGDIKLRNARYRDDTRPLDEGCACHTCRNFSRAYLHHLHRAGEITG
                     ARLNTLHNLHYYLQLMQEMRDAIEKGEFDSWRTRFAENRAIGI"
     gene            12355..12708
                     /gene="yajC"
                     /locus_tag="VFR20_12125"
     CDS             12355..12708
                     /gene="yajC"
                     /locus_tag="VFR20_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU0231311.1"
                     /translation="MSVASTLQSITAQAGGSAAGGENALMGMLPIILMFVILYFLMIR
                     PQMKRQKDHRNMVSALSKGDEVITSGGVLGKVTKVSDNYITVEIGSVSDKPVEAVMQR
                     TAITSVLPKGTIKAL"
     gene            12769..14643
                     /gene="secD"
                     /locus_tag="VFR20_12130"
     CDS             12769..14643
                     /gene="secD"
                     /locus_tag="VFR20_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEU0231312.1"
                     /translation="MNRYPLWKYLTVLAALLIGILYTLPNFFGESPAVQIAADKATVK
                     IDNATLERVQDILAKDKIPTTGAYFQQNGPNGTVRVRFANTDIQLKAKDTLAKALNPD
                     PNDHGYTVALNLLSSSPNWLSDLGAHPMYLGLDLRGGVHFLLQVDMHGALTGRYDSLA
                     SDIRTALRDANIKTSGVERDGLSVLANFDSADARDNAVSELRRSIPNMQFVKGAPNAG
                     KYPLTGTLTSSAITEVQTHAIKQNIQTLHNRINELGVAEPVIQQQGSDRIVVQLPGVQ
                     DIAKAKEILGRTATLQIRMVDDSPSAIAALNAGKVPFGLERYTDANGQPILLRRQVIL
                     TGENLQDAQPGRDPQTQEPTVNLTLDPKGARIFRDVTRNNIGKRMAIVLVENGKGQVV
                     TAPVIRSEIPGGHVQISGAMTAQEAADTALLLRAGALAAPMKIIEERTIGPSLGADNI
                     SKGFHSTLWGFVAIAAFIMLYYRLFGAFSTVGLAFNVLLLLAVLSMLQATLTLPGIAA
                     IALTLGMAIDSNVLINERIREELRLGLPPQQAIHLGFQRAWDTIFDSNLTTLIVGLSL
                     LAFGTGPIRGFAVVHCIGILTSMFSSVVGVRALANLWYGRRRKLKTISIGQIWKPNKD
                     "
     gene            14697..15632
                     /gene="secF"
                     /locus_tag="VFR20_12135"
     CDS             14697..15632
                     /gene="secF"
                     /locus_tag="VFR20_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEU0231313.1"
                     /translation="MEFFRIHRTIPFMRHALVLNIISLVMFILAVFFIVTRGFHLSIE
                     FTGGTVMELHYTQTAQTEKIRQAIDTLHYADFQVQNFGTSHDIMVRLPLRPKETSSEQ
                     SQAVLKVLKAQTPNVELRRVEFVGPQVGQELVHNGLMALLCVVVGIMIYLAFRFEWKF
                     AVSGVIANLHDVVIILGFFAFFQWEFSLSVLAGVLAVLGYSVNESVIIMDRIRENFRK
                     QRKAPTREIINSAITQTISRTIITHGSTQMMVLSMLFFGGPSLHNFALALTIGICFGI
                     YSSVFVAAALAMWMGVKREDMIKTVKKDDPRAEIA"
     gene            15708..17126
                     /gene="miaB"
                     /locus_tag="VFR20_12140"
     CDS             15708..17126
                     /gene="miaB"
                     /locus_tag="VFR20_12140"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEU0231314.1"
                     /translation="MQDTTSPSPDTASGDSIAPRDASRPRKLFIRTFGCQMNEYDSDK
                     MADILHADQGIERTNTPEDADVILFNTCSVREKAQEKVFSDLGRVQHLKRENPDLIIG
                     VGGCVASQEGEAIVRRAPYVDVVFGPQTLHRLPDLIARRRASGQPQVDIRFPEIEKFD
                     AMPPARVEGPTAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFDDVLVEVADLADQGVRE
                     VTLLGQNVNAYRGAMGGTDDIADFAMLLEYVHEIPGIERIRYTTSHPKEMTTRLIEAH
                     GKLDKLVPFLHLPVQSGSDRVLAAMKRGYTSLEYKSIVRRLRAARPGLSLSSDFIIGF
                     PGEGEAEFEKTLDLIRHVGFDQSFSFVYSRRPGTPAADLDDDTPQALKLERLHRLQAL
                     INDQAAKISHDMVGTIQRVLVEKPSRRDPNELTGRTADNRMVNFAGHRRLIGQMMDVR
                     VVQALTNSLRGEVPEATAGATA"
     gene            17123..>18093
                     /locus_tag="VFR20_12145"
     CDS             17123..>18093
                     /locus_tag="VFR20_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU0231315.1"
                     /translation="MSVDSRAPVIVDLGGDNTHLANLCGPLDENLKQIAIGFDVQLSR
                     RGTHLTLRGAQAERASQAVEWFHRRAVHQALSVDDIQLGLIELDSGHKSKSPAKHPDT
                     PAPGEPDDDGPLLHTRKNDLRPRTPRQRDYLHGILKNDITFGYGPAGTGKTWLAVACA
                     IDALERQTVERLILTRPAVEAGERLGFLPGDLAQKVDPYLRPLFDALYDLMGFDRVQR
                     LFDKQSIEIAPLAYMRGRTLNHAFVILDEAQNTTPEQMKMFLTRIGFGSKAVINGDPS
                     QVDLPKGQRSGLGHAIRLLDDVPGIAFTQFTSRDVVRHPLVARIVDA"
BASE COUNT         3421 a         6222 c         5356 g         3094 t
ORIGIN      
        1 acataggctt ccatgtcggc gatgggccgg gtggccaccc cgtcgtccat ggcggccttc
       61 gccaccgccg gcgcgatgcg cacgatgagg cgcgggtcga acggtttcgg gatgaggtac
      121 tcgggcccga acgacacgcc ataggtgcca taggccgccg ccaccacgtc gttctgttct
      181 tcctgggcga gccgggaaat ggcctcgacc gccgcttttt ccatgccgcg cgtgatggtg
      241 gtggcgccca cgtcgagcgc gccgcggaaa atatagggga agcacaggac gttgttgacc
      301 tgattggggt agtcggagcg acccgttgcc atgatcacgt cgtcacgcac ggcacgcgct
      361 tcctcgggca ggatttcggg cgtggggttg gccagggcca ggatgatggg ccgttcagcc
      421 atcttcgcca ccatctccgg cttgagcaca ccgcccgccg acaaccccag gaagacgtcg
      481 gcgccgtcaa tgacatcggc cagcttgcgc gcgccggttt cctgtgcgaa ccgttccttg
      541 tcagggtcca tgagcgccgt gcgccccttg tacacgaccc cttcgatgtc ggtcacccag
      601 atgttcttga ggggcaggcc cagatcgacc atgagatcga gacaccccag cgcggcggcg
      661 cccgcccccg acaccacgac cttgacctga tcaatggcct tgcccgcgac ccgcaggccg
      721 ctgatgaacg ccgacgagac gcagatggcc gtgccatgct ggtcatcgtg aaacaccggg
      781 atgcccatgc gctcgcgtag cttgcgctcc accgtgaagc attccggtgc cttgatgtct
      841 tcgaggttga tgccgccgaa cgtgggctcc agcccggcga tgatgtccac cagcttgtcc
      901 ggatcggttt cgttgatttc tatgtcgaac acgtcaaggc cggcgaactt cttgaacagg
      961 accgccttgc cctccatgac cggcttggag gccagggggc cgatattgcc caggcccagc
     1021 acggccgtac cgttggtgat gacccccacc aggttgccgc gcgcggtata gcggaacgcg
     1081 ttcttgggat cggccacgat ttcttcgcac gcggccgcca cccccggcgt ataggccagc
     1141 gccagatcgc gctggttcga caactgcttg gtcggtgtga ccgagatctt gccggggcgt
     1201 ccgtgctcgt gatattccag ggccgctttg cgaaggttgt cttccatggt ggtttcttga
     1261 tggcgcgtga gtgagaagaa agaagctcat tctagcccta gtgcccgctt tcgcgcacca
     1321 tcgtggaaat ccgtgtgcga aattccgtct atgcggattc ggcatgggct cggcgtcccg
     1381 ctcgaacgcc cggcggcgta acactgcggg gcggcttaca gattctgccc tgggaaggga
     1441 aaaaggtttg atggatcaaa cacatacttc acctgagagt cggcatcgga ggccgcgagc
     1501 caggtgtcat cgtcgtgcgc ctcgtgattg gtcgagaacc ggggaaaata agaggcctcg
     1561 atctcgttgt cgacgcgctc atccaacacc acccagtcta cccagccaag gtcgccgcca
     1621 tgtccaagca gaaggtgtgc cagattgacg gtctggccgg gcaccggcaa gagggcgtcc
     1681 aggcggtagc gggcgggcca gaactcctgt ccgcgacacg tttgcgcagc ctgcgaagtg
     1741 gactgttcga acaatgccgg caccagacgc tgcagagtga gcccttccag gggttgatgg
     1801 ctgttctcgc cgaacggccc cagcgtggcg cgcgttccat cggccagcat gacgctggca
     1861 tgctgcaggc ccgacttgta cgtttccccg gtgcgccaat cacacagccc acgatcggcc
     1921 agccaggccg cgacggtgag atgcgcgggc agatcccgga actgacgcag ccccacagcc
     1981 tcgagttcgc caagcatgca gccgggctgc acgaaccagt aggggctgtc atcggcgatg
     2041 cgcttgcact ccgccatgtc gcgtcccggg tctatccaca acacgggaaa atcgtcgagt
     2101 gcgctggcgt gctggacacc atcgagcgcc atggtgacgc catgctcgac gcacagttcg
     2161 cgcgcgcgct ggacgtcgga tgaatgcgct ggggtaaggc gcgcgctacc cacgccattc
     2221 tgttcgccga aatcatagaa cgtcccttcc atcgcccggt tcatatgctg acagaaggct
     2281 tcccaaggcg tgcgcaaggt gcgccgcccc atgagaaagg cccggcggga attggatggc
     2341 ataacgcagc attcctatcg aacgatggcc gcgatcggcg tgaagcaccg ttcgcaagct
     2401 ctacaatggt cacttttatc tggaagcccg accccatgcc ccgtctcgcc cagcgcaccg
     2461 accgtgtcat gccattttat gcggtggaac tcttcaagca agccggcgcc ctgaacgcgc
     2521 aaggccgcga catcatcagc ctgggaatcg gcgaacccga ctttaccgca cccgcacaag
     2581 tcgtggaaac cttgcgacgc gcggccgaag ccggtctgag cggctacacc gcgccagccg
     2641 gcctggccac gctgcgcgaa gccatcgccc attactatgg ggaacatttt tcggccccgg
     2701 tcgatccgga tcgtgtcatc gtgacatcag gggcgtcggg cgcgttgctg ctggccagca
     2761 tggcgctcat caacccgggc gacgaggtcc tgatgcccga cccatcgtat ccggccaacc
     2821 agaatttcat caccagcgcg gggggcgtgc cacgcctgat tccctgccag gccgagcaga
     2881 atttccagct cagcgcggcc gacatccgcg cccaatggac gcccgccacg cgcggggtgc
     2941 tcatcgcctc gcccagcaac ccgaccggca ccaccatcgc ccgcgaggac ttgaaagccc
     3001 tcgtcgccga agtccgcgcg cgcgatgggt tcgtcatcat ggacgaaatc tatctgggcc
     3061 tgtactacgg cgacaagccg cagagcgccc tgacactgga tgacgacatt gtcatcatca
     3121 acagtttctc gaagtacttt cacatgacgg gctggcggct gggctggctg atcgccccac
     3181 cctcgatgat tccggccgtg gaaaagctgg cggccagcct ggcgatctgc gcccccactc
     3241 tggcgcaaca tgccgcgctg acttgcttcg agccggatgt catgcgtgtt tacgagcacc
     3301 gccggcaatc attccagcag cgccgcgact atttgttgcc ggaattcgaa cgcctgggcc
     3361 tgcatgtgcc ggcgcgtcct gacggtgcgt tctacattta cgccgacatc cggaacttca
     3421 gccaggacag cgatgatttc tcacatcgcc tgctgcacga agccggcgtg gcggccgtgc
     3481 ccggccgcga cttcggcgag gcacacgcgc gccacacaat gcggttttcc tatgcgacag
     3541 ggttagatcg gctgcaagag gcagtggaac gcatggagcg gttcctggag cgctgaaacc
     3601 cagctgtgct ccgtcccccc gtttctttag ggccacgaag gcacggggag acaaagctgc
     3661 gccttccaac agaagccact actggctact tgttcgagat cggaattccc gaagccaatg
     3721 ccagccgacg ctgttccgcc atgaccctgg ccccgtaccc cccatcgcca gggcccgttg
     3781 cccccacgta acacgccagc cctccggaca ccgagccccg ccgcttgatg cactcatcca
     3841 gaatctgcgc gccgacacga atgttcgcga tggggttcag cgccgccgcg gcgccttcgc
     3901 caaaacggtc gaacttgtcg atgtgcacct gggtcatcac ctgcatcaac ccctgcgccc
     3961 ccacatggct ctcggcatac gggttgtagc cggactcgat cgcgatcacc gccagcaaca
     4021 actgcggatc gagatgcttt tgccgcccca cctggaacgc ggtcttcacc aatacccccg
     4081 ccacactgga cgcgatcttg tatttgcgcg cgatgtaact gcgcagcgcc tgggcttgcg
     4141 catgactgat cccttttact tccagcccat gcacgctggc cttgagctca cgcacgaaac
     4201 tctgatgcag cgcgggggcg gatttcaccg ccccggtggc acccggcttt ccacccgcgg
     4261 actcggcctc tgcctggggg gacgtgcctg ccgccgcccc agtggtggcg gtggctccgg
     4321 tggccccagt gttagtggtg gctccagtgg gcggcaagcc ttcctgccct gatgcctgag
     4381 gcggttgggc ttcgttgttg gcctcttgaa gaagcatctc gtactgggcg aggtcattcg
     4441 taccggactc gtcattttgt attgagtccg gctgcaatgc cgccaacagc gccgagtgca
     4501 cctgccgagc ctgctcacgc acagacggaa ccgtcaggca caacgccagc aacaccagcg
     4561 cggcaatgcc caggtagacg gcggccacat gcaccagttc acccagcggg agcgcaatag
     4621 agcgaaccat caggcccagc gggttttccg agcgggttgc gcccatgcgg ttccttccca
     4681 ataaaatacc gacaaccgat gttaacctca gcaagaccaa cggatgtaac gggatttgac
     4741 atttgtcctt ccgtattccc ccacccccag ggcccaccag tgaaataccg agacctcaga
     4801 gacttcatcg cacagcttga acgagcgggc gaactcaaac gcgtggccgc cagggtggac
     4861 acggatctcg aaatcaccga gatctgtgac cgggtgctgc ggcgcgccgg ccccgccctg
     4921 ttgttcgagc accctgtgca ccagggcgcc gacgccgcca tgccggtgct tgccaatcta
     4981 ttcggcaccc cccgccgcgt cgcctggggc atgggtgccg acgacgtggg cgccctgcgc
     5041 gaaaccggcg aactgctggc ggcactgcgc gaacccgagc ctccgaaagg tttccgcgac
     5101 gcgctggcca aggtgtccat gctcaaggcc gccttgtggg acatgaaccc caggacggtc
     5161 aagaatccac cctgccaaga catcgtcctc gaaggcgccg acgtcgatct ggaccgcatt
     5221 ccgctgcaac gatgctggcc cggcgacgtg gccccgctgc ttacctgggg gctggttgtc
     5281 acccgcggac cgaacgcccg gcgccagaac ctgggcattt accggcagca gcgcctgggc
     5341 aggaacaagc tgatcatgcg ttggctgtcg catcggggtg gcgcgctcga cttccgcgac
     5401 cacatgttgg cgcaccccgg tacaccgttt cccgtggccg tggccctggg cgccgatcca
     5461 gccacgattc tgggcgccgt cacgcccgtt cccgacacgt tgtcggaata ccagttcgcc
     5521 ggtctgctgc gcggttcgcg caccgaggtc gccaagaccc tgggcagcga gctggacgtg
     5581 cccgctttcg ccgaaatcgt gctggaaggc catttgctgc cgacttcaga cccacgcgcc
     5641 atgcggccgg acgtaccgga aggtgtttcg cccccgccgg acaacggcta cgaaatggcg
     5701 ctggagggcc catacggcga tcacacgggc tattacaacg aacaagactg gtttccggtt
     5761 ttcaccatcg atcggatcac catgcggcgc gacccgatct atcacagcac ctacaccggc
     5821 aaacctcccg acgaacccgc cgtgctgggc gtcgccctga acgaagtctt cgtgccgttg
     5881 ctgcgcagac aactgcccga gatcgtcgac ttctacctgc cgcccgaagg ctgcagctac
     5941 cgtctggccg tggtatcgat acggaaacag tatgccggcc atgccaagcg ggtcatgttc
     6001 ggcctgtgga gcatcctgcg ccagttcatg tacaccaagt tcatcgtggt ggtggaccat
     6061 gacatcgatc cgcgtgactg gaaggaggtg gtctgggcca tgaccacccg catggacccg
     6121 gtgcgcgacg cggtgctggt ggaaaacacg cccatcgact acctggattt cgcctcgccg
     6181 gtatccggac taggcggcaa gatgggactg gacgccacga acaaatgggc cggcgagaca
     6241 tcccgccaat ggggcatccc catacaaatg gaccgcgccg tgaaggataa agtggacgcg
     6301 ctgtgggatc agctgggact gtgacgtcct ggatcagtcc ctgggccgcg agaacaggcg
     6361 cgccgcctct attccggcca gcaaggcggc ccccaggcgc caccagcggc ggcggcgccc
     6421 gcccgccccc gaaaacgcgg ccgacacggc ggaaaccaac agaggatagc gtcgcgccag
     6481 cgcggccccg ttgcgcagcc aagccagagg cccgtgcccg cgtcccagcg gcgacagaaa
     6541 ccgggacacg atgcggcctg gcgacagttc ttgaaccgcg tcgcaggcgt cacgcttgag
     6601 acgctcgcgt tccacggcgg cgcgggcgcg ggcgagctcg atccgcatca cccgttcgtt
     6661 gcgggcggag acgcctttca tggggaattc tccggagcac catcccgacc gtctgcatgc
     6721 gccgcgccgg gctcgcgcaa acgcccgaac agggccgcat cgcgccgcag ttcgtcaagc
     6781 gtggcggaga acggcaacgg atcgccgcgc aatcgcaccc gtaccgtcca ggcgaacgcg
     6841 atgcccagaa ccagatacaa cagccccagc aaccacaacg ccatataacg atagggggtt
     6901 ggccagaaat acagcgccac cgtcaccgac agaaccagca ccgccagcgc caggaagaac
     6961 gccgccgcgc acagcaaggc caataaacca atcaaccgcg ctttttgttc acccgcttcg
     7021 agggcgaaca attcaagccg ggtacgcgcc atggacagca aggtgccggc caggtcgccg
     7081 acagcgcgac taagtgccat attcctgaag cctgttcaca tgagaaggcg attgccgcgc
     7141 ggcgttcacc ggcgggaaat cagcatgccg accagcaatc cggccacgct ggccatgcca
     7201 atggcttgcc agggattgtc gtgcacgtag tcatcggtgg cgcgggccgc tcggcgccct
     7261 ctttccatca gggcatcctg ggtgtcatac agcgtttcgc gagtgtgccg cagcgaggtc
     7321 attgcccgct cgcgcaattc ggcggccttg tcgccggtgg tactggccgc gtcccgcaaa
     7381 agctgctcgg catcgtccaa gctggccttc aggctctcga tgaaatcttc ttcagttgct
     7441 ttggcgtctg gtttcttggc gctcatggtc atctcctgca ttggaaatcg gaaacacccg
     7501 ccagcgccgg ataatctgac tgaggagtcc ctcgctgacc gacaggtagg cggcgggggc
     7561 cccattcagg gtggacaggc actagtctgg gccgctcatg accgagttta cagattccag
     7621 cgccaaagac aaccgcgtca agcctctttc tggcgggttc cgaaaatccg gtcgcccgcg
     7681 tcgccgaggc ccggaatgat gtaaccattc ttgttcaggt ggctgtcgat ggacgccgtg
     7741 tagacatgca catccggatg cgccgcctcg acggctgcga tgccttccgg cgccgccacc
     7801 agaaccaacg cgcgaatatt gacgctgccg gctcgcttca gcatatcgat ggtggccacc
     7861 attgatccgc cggtcgccag catgggatcg acgatcaacg ccaggcgctg gtccagcccg
     7921 cccacgagtt tctcgagata cggctccgcc tgcagggttt cctcgttgcg ggcaatgccg
     7981 accacactga ccttggcgcc cggaatgagg gtcaacaccc cgtcgagcat gccgatgccg
     8041 gcacgcagaa tgggcaccac cgtgaccttc ttgcccgcca gtttctcaac cgtgacctcg
     8101 ccggaccacc cttgtatcac gtggggctcg agcggcagat ccttggaagc ctcgtaggtg
     8161 agcaacgcgg cgatctcctg tgccatttcg cggaaattct tggtgctcag gtcgccacgg
     8221 cgcatcaagc caagcttgtg acggatcagg ggatgacgga tttcgtgtat gggcatgtcg
     8281 cgggaatttt tatgaaaagt caggaatcgg ccagccaggc gatcacggcg tcatcgaagg
     8341 gccgagcggc gccggcgcgg tcatcgttga cgaaactcac cacaatatac cgcttgccgc
     8401 tctttcccag cacgtagccc gccagggagc aggcattgcg caaggtgccg gtcttgagat
     8461 gcgccgcgcc acgagtaccg tcgctgcgca gacggtgctt catggtgccg tccatgcccg
     8521 atatggacag cgacgagacg aattccggca tcagggacga ccgccaggcg gtgtcgagca
     8581 tgctgaccag cccgcccgcc gtcaggcggg acccacgcgc cagtccggcg ccattgacga
     8641 tatgccaccc ctgggtgtcc acacgctggg acgccagaat ggccaaggcg gcattggcgc
     8701 ccgaggccgg cgtcgccccc gggccgttct tttccgcgcc gagcgtcagc aacagcgtgc
     8761 gcgccatgac gttgttgctg tgcttgttga tggcgcgtat ggtgtcggac aacgatacgg
     8821 aatcgtgcca gacgatgggt tgggcggagg gtggcaccct gccggcgcga aaccccttgg
     8881 ccagcgtgcc gcccagatcc agccacaaca tccggaacac cgcgtcgaag tagtcgggct
     8941 gcgacatcgc cagcctgtac acgctgaact cgccacacga ccccgccgcc gtgccgtcta
     9001 cgtgaatcac gacgccttgc gcggaagccg tttcagtcac tcccacggtt ggcgacccgg
     9061 ggcagcgcgc attgctccac ttcacgttgc cttcaacccg cagcccacgc agtggcgggt
     9121 cgatgatggg aacccatttc ctggcggccg cgtccggctc gaacagcaag cgcaccgcgc
     9181 ccaggctcac catcatggca tccgggctgg cgttgtaggg ccggtagggc gagtcgtcga
     9241 aatcgccggg atcggtggcg acgttgccga agagggaacg gtcgaccacg atttcattca
     9301 ggttcttgat gccgcgcaac cgcagctcac gcagcagatt ccatagatcc tgaatcgtca
     9361 ggaaaggatc cccacccgcc ttgacataca acggtccgcg caatgtgccc tgcgcgtcga
     9421 cccgcgcgcc atcacgcccc agcaacgtgg tgcgccattg gtaatcgggc cccaggcccg
     9481 acaggccggt ccaggtcgtg accattttca tgacagaggc ggggttgcgc gacgtttggg
     9541 gattgacgga caccatgcgg ctaccgccca cttcctggac caccagcgac aacgcgcttt
     9601 gcggcaaacc tgtggcctgc cacgcttttt gcaattgcgg cggcaaagcc tgggccatcg
     9661 caccggcgct gcccagcgac agagccaccc ccgcgaggaa agccagccag tcgcgccgtg
     9721 ccgcgcggaa gaagcttgtt gaatttcccg aacgaaccat ggttagttac gcgcctctcg
     9781 acccgccgac atgaaaaaac acaatttttt gccgccgggc caccccaggc aaaaaaacat
     9841 cagcataccc aaagatgccg cgccgcgcgc gggccatcgg ctgaaagtta catcaaccgc
     9901 gtaaaatacc cgcttccgaa ttgcggcgcg caccatgcgc cactcccccc tccttacccc
     9961 ccacgcctgt gaacaccacg ctgtctctct cacaattcga ttaccacctc ccccaggaac
    10021 tgatcgccca gacccccgcc gaccggcgca caggcagccg cctgctgcac ctggatgggc
    10081 acggcgcgct ccatgaccga cagttcaccg acttgccgga cctgttgcgc cccaatgacc
    10141 tgctggtgtt caacgacacg cgcgtgatca aagcccgatt gatgggccac aaggaaaccg
    10201 gcggcaagat cgaagtcctg gtcgagcgca tcacaagtcc acgcacggcg ctcgcgcaca
    10261 tccgctccag caagtccccg cgcgccggct cgcgggtggt cctggccgac gccttcaccg
    10321 ccaccgtgac gggccgccag ggtgaactgt tcgagctgga cttcccggaa cgcgtgctgg
    10381 acctgcttga acggcacggc gccaccccgc tgccgcccta catcacacac ccggccgacg
    10441 cccaggacga agaccgctac cagaccgtct acgcgcgcgc gcccggcgcc gtcgccgcgc
    10501 ccacggcggg cctgcacttc gatctgcccc tgctcgaaga gatcggccgt cgcggcgtgg
    10561 gcaaggcatt cgtcacgctg cacgtcgggg cggggacatt ccaacccgtg cgcgtcgacc
    10621 ggatcgacca gcacgtcatg cattccgagc actatgtcgt gccagccgag gtcctggagc
    10681 agatcgccgc cacgagggca aacggcgggc gcatcatcgc ggtcggcacg accagcgtgc
    10741 gcgcgctgga atccgtcgac caggatgcgg cacccgatgg gcccgccgaa ggcgacaccc
    10801 ggttgttcat cacccccggc tacccattca agcgcgtcga tgccctgatc accaacttcc
    10861 acctgccgca gtccaccctg ttgatgctgg tatcggcgct ggcgggcatg gaacccatac
    10921 ggcgcgccta cgcccacgcg gtcgacgcgc gctatcgctt cttcagttat ggcgacgcca
    10981 tgttcataga gtcatccgca tcatgaccgg acttgatttc acactcctgg ccactgacgg
    11041 cgccgcgcgg cgcggccgcc tcgtcctcaa ccacggtgtc gtgcaaacgc ccatcttcat
    11101 gccggtgggc acgtacggca gcgtcaaggc catgatgccg catgagctcg acgagatcgg
    11161 cgcgcagatc gtcctgggca acacgttcca tctgtggcta cgccccggca ccgacatcat
    11221 cgatcggcac gacgggctgc atggattcat gcggtggccc cgccccattc tgaccgattc
    11281 cggtggcttc caggtgttca gcctgggcgg actgcgcaag atcaccgagg aaggggtgcg
    11341 gttcgcctct cccatcgacg gggcgcggct tttcctctcg ccggaagaat cgatgcgcat
    11401 ccagcgttca ctcaactccg atatcgtcat ggtgctggat gaatgcacgc cctattccat
    11461 cgacgggcgg ccggccaccc tggacgaggc cgcgcgctcc atgcgtatgt cgttgcgctg
    11521 ggcgcggcgc tcgaaggaag cgttcaatcg gctggagaac ccgaacgccc tgttcggcat
    11581 cgtgcaaggc ggcatgttcg aggcgctgcg cgacgaatcc ctggcgggcc tgaccgatat
    11641 cggcttccac ggctacgcca tcggtggcct gtcggtgggg gagcccaagg aagagatgct
    11701 gcgcgtcctg tcgcatgtgg cgccacgcct gccggcgcag gcaccacgct acctgatggg
    11761 cgtgggcaca cctgaagacc tggtcgacgg cgtggcctcg ggtgtggaca tgttcgattg
    11821 tgtcatgccc acgcgcaacg cccgcaacgg ctggctgttc acccggttcg gcgacatcaa
    11881 gctgcgcaat gcccgctatc gcgatgacac ccgccccctg gacgaaggtt gcgcctgcca
    11941 tacctgccgg aatttctccc gggcgtatct gcaccacctg caccgcgccg gagagatcac
    12001 cggcgcgcgc ctcaacactt tgcacaacct gcactattac ctgcagctca tgcaggaaat
    12061 gcgcgacgcc atagaaaagg gcgaattcga ctcgtggcgg acccgtttcg ctgaaaaccg
    12121 ggccatcggc atctagtgtg ctgccgccga agtacgcatc catgaaatcg catcttcacc
    12181 cccatggcag cgttgcaaat cctcgcgata gcattggcta tcgctgcggt tcgcgccttg
    12241 ccctgggggc gaatccatcg atttcataat ggctccgtac ttcggcggca gcacacaagt
    12301 agcggatacc gctacaatac agggtttatt gaaaatttct ttcaggagtg ccgaatgtcc
    12361 gtagccagca cactacaatc gatcacagcg caggccggcg ggtccgcggc cggcggggag
    12421 aacgcgctga tgggcatgtt gcccatcatc ctgatgttcg tcatcttgta tttcctgatg
    12481 atacgccccc agatgaaacg ccagaaagac caccgcaaca tggtgtccgc cctgtccaag
    12541 ggtgatgagg tcatcacctc gggcggcgtt ctgggcaagg tcacaaaggt cagcgacaac
    12601 tacatcacgg tggagatcgg ctccgtctcc gacaaacctg tcgaagccgt catgcaacgg
    12661 acggccatca cctcggtcct gcccaagggc accatcaagg cgctgtaatc ccgcgcaccg
    12721 cccggctgtc cgccgggcgg cccttccgca cccgtcacct ggctgataat gaatcgttat
    12781 cctctctgga aatacctcac cgtactcgcc gcgctgctca taggcatcct gtacaccctg
    12841 cccaatttct tcggtgaatc gcccgctgta cagatcgccg ccgacaaggc cacggtgaag
    12901 atcgacaatg ccacgctgga gcgggtccag gacattctcg ccaaagacaa gattcccacc
    12961 acgggcgcct acttccagca gaacggcccc aacggcacag tgcgtgtgcg tttcgccaat
    13021 accgacatcc agctcaaggc caaggacacc ctcgccaagg cgctcaaccc cgaccccaac
    13081 gaccacggct acaccgtagc cctgaacctg ctgtcctcat cacccaactg gctttccgac
    13141 ctgggcgcgc acccgatgta cctcgggctg gacctgcgcg gcggggtgca cttcctgctg
    13201 caggtcgaca tgcatggcgc cctcaccggc cgctacgact cgctggccag cgacatccgc
    13261 acggcgctgc gtgacgccaa catcaagacc tcgggcgtcg agcgcgacgg tctgtcggtg
    13321 ctggccaatt tcgacagcgc cgatgcccgt gacaacgccg tctcggaatt gcgccgcagc
    13381 atcccgaaca tgcagttcgt caagggcgcc cccaacgcgg gcaagtatcc gttgaccggc
    13441 acgctcacca gttcggccat caccgaagtg cagacacacg ccatcaagca gaacatccag
    13501 acgttgcaca accgcatcaa cgaactcggc gtcgccgaac cggttatcca gcagcagggc
    13561 tccgaccgca tcgtcgttca gcttcccggt gtccaggaca tcgccaaggc caaggaaatc
    13621 ctcggccgca cggccacgct gcaaatccgc atggtcgacg actcgcccag cgccatcgcg
    13681 gcgctcaacg cgggcaaagt tccctttggc ctcgaacgct acaccgacgc gaacgggcaa
    13741 ccgatcctgc tgcgccgcca agtcatcctg accggtgaaa acctgcagga cgcccagccc
    13801 ggccgcgacc cgcaaacgca ggaacccacg gtcaacctca cactcgaccc caagggcgcg
    13861 cgcatcttcc gcgacgtcac gcgcaacaac atcggcaagc gcatggccat cgtgctggtc
    13921 gaaaacggca agggccaggt cgtgaccgca ccggtcatcc gcagcgagat cccgggcggc
    13981 catgtgcaga tttccggcgc catgaccgcc caggaagcgg ccgacaccgc gctgctgctg
    14041 cgcgccggcg ccctggccgc ccccatgaag atcatcgagg agcgcaccat cggcccgagc
    14101 ctgggtgccg acaacatctc caaggggttc cattccacgc tttggggttt cgtcgccatc
    14161 gccgccttca tcatgctcta ctaccgcttg ttcggcgcgt tctccaccgt cggcctggcg
    14221 ttcaacgtgc tgctgctgct ggcggtgctg tccatgctgc aggcgaccct caccttgccg
    14281 ggcatcgcgg ccatcgcgct cacgctcggc atggccatcg actcgaacgt gctcatcaac
    14341 gagcgcatcc gtgaagaatt gcggctcggc ctgccgccac aacaggccat acatttgggc
    14401 ttccagcgcg cgtgggacac gattttcgac tccaacctca ccacgctcat cgtcggcctg
    14461 tcgctgctgg cattcggcac cggccccatc cgcggtttcg ccgtcgtgca ttgcatcggc
    14521 atcctgacct cgatgttctc gtcggtcgtc ggcgtgcgcg ccctggccaa cctctggtac
    14581 ggacgccgcc gcaagctgaa gacgatctcc atcggccaga tctggaaacc caacaaggac
    14641 tgaacccgct cggcgcgcac ggcgcgccgc ggtcgcaacg aaactaggcg agactcatgg
    14701 aatttttccg catccaccgc accattccgt tcatgcgcca cgcgctggtg ctcaacatca
    14761 tcagcctggt gatgttcatc ctcgcggtgt tcttcatcgt cacacggggc ttccatctgt
    14821 ccatcgagtt caccggcggc acggtgatgg aactacatta cacccagacg gcgcagacag
    14881 aaaaaatccg ccaggcgatc gacacgctgc actacgccga cttccaggtg cagaacttcg
    14941 gcacctcgca cgacatcatg gtgcgcctgc cgttgcgccc caaggaaaca tcttccgaac
    15001 aaagccaggc cgtgctcaag gtcctgaagg cgcaaacgcc caacgtcgaa ttgcggcgtg
    15061 tcgagttcgt tggcccccaa gtcgggcagg agctcgtgca caacgggctg atggccttgc
    15121 tatgcgtggt ggtcggcatc atgatctacc tcgccttccg tttcgagtgg aagttcgccg
    15181 tgtcgggggt catcgccaac ctgcacgacg tggtcatcat cctggggttc ttcgcattct
    15241 tccagtggga attctcgtta tccgtgttgg ccggggtgct ggccgtgctg gggtactcgg
    15301 tcaacgaatc cgtgatcatc atggaccgga tccgtgaaaa tttccgcaaa cagcgcaaag
    15361 cgcccacacg cgaaatcatc aacagcgcca tcacgcagac gatttcgcgc accatcatca
    15421 cccacgggtc gacgcagatg atggtgctct ccatgctgtt cttcggcggc ccgtcgctgc
    15481 acaacttcgc cctggccctc accatcggca tctgtttcgg catctactcg tcggtgttcg
    15541 tggcggcggc cctggccatg tggatgggcg tcaaacgtga agacatgatc aagaccgtca
    15601 agaaagacga cccgcgcgcg gaaatcgcct gacatcacca cgcatgcggg taaaataccc
    15661 gcatttcccc tgaagccccc gggatacacc ccgttacggc agccatcatg caagacacca
    15721 catccccgtc gccggacacc gcgtccggcg attcgatcgc accacgcgac gcatcccgcc
    15781 cacgcaaact gttcatccgc accttcggct gccagatgaa cgaatacgat tccgacaaaa
    15841 tggccgacat cctgcatgcc gaccagggaa tcgaacgcac caacaccccg gaagacgccg
    15901 acgtcatcct gttcaacacc tgctccgtac gcgagaaggc gcaggaaaaa gtgttctccg
    15961 acctgggccg cgtccagcat ctgaagcgcg agaaccccga tctcatcatc ggcgttggcg
    16021 gatgcgtggc cagccaggaa ggcgaagcca tcgtccggcg cgccccatac gtggacgtcg
    16081 tgttcggccc acaaacgctg caccggctgc ccgacctgat cgcgcgccgc agggcatcgg
    16141 gccagcccca ggtggacatc cgcttccccg agatcgagaa attcgacgcc atgccgcccg
    16201 cgcgcgtgga gggccccacc gcgttcgtgt ccatcatgga gggttgcagc aagtactgca
    16261 gcttttgcgt ggtgccctac acgcgcggag aggaagtctc gcgccccttc gacgacgtgc
    16321 tggtcgaggt ggccgacctg gccgaccagg gcgtgcgcga agtgaccctg ctgggccaga
    16381 acgtcaacgc ataccgtggc gccatgggcg gcaccgacga catcgcggac ttcgccatgc
    16441 tgctggagta cgtgcacgag atcccgggca tcgaacgcat ccgctacacc acgtcgcatc
    16501 ccaaggaaat gaccacgcgc ctgatcgagg cgcacggcaa gctcgacaaa ctcgtaccgt
    16561 tcctgcatct gcccgtgcag tccggcagcg accgcgtcct ggcggccatg aagcgcggct
    16621 acacctcgct ggaatacaaa tccatcgtca ggcgcctgcg cgcggcgcgc cccggcctga
    16681 gcctgtcatc cgatttcatc atcggattcc ctggcgaggg tgaagccgaa ttcgagaaaa
    16741 ccctggatct catccgtcac gtggggttcg accagtcatt ctcgttcgtg tattcgcgac
    16801 gcccgggcac ccccgcggcc gacctggacg acgacacgcc gcaggcgctc aagctcgaac
    16861 gcctgcaccg cctgcaggcc ctgatcaacg accaggcggc caagatcagc cacgacatgg
    16921 tcggcacgat tcagcgtgtt ctggtcgaga aaccctcgcg ccgcgatccc aacgaactga
    16981 ccgggcgcac cgccgacaac cgcatggtga acttcgccgg ccatcgccgc ctcatcggcc
    17041 agatgatgga cgtgcgcgtc gtccaggccc tcaccaactc cctgcggggc gaagtgcccg
    17101 aggccacggc cggagcgacg gcatgagcgt ggactcgcgc gcgcctgtca tcgtcgatct
    17161 gggaggcgac aacacccatc tcgcgaacct gtgcggcccg ctggacgaga acctgaaaca
    17221 gatcgcgatc gggttcgacg ttcagctcag ccgccgcggc actcacctga ccctgcgcgg
    17281 cgcgcaggcg gaacgcgcaa gccaggccgt ggaatggttc caccgacgcg ccgtgcacca
    17341 ggcgctgtcg gtcgatgaca tccagctcgg cctcatcgag ctcgactccg gtcacaaaag
    17401 caaaagcccc gcaaaacacc cggacacgcc ggcgccgggc gagccggacg acgacgggcc
    17461 gctgctgcac acccgcaaga acgatctgcg gccacgcacc ccgcgccaac gggattacct
    17521 gcacggcatt ctgaagaacg acatcacgtt cggctacggg ccggcgggca cgggcaagac
    17581 ctggctggcc gtcgcctgcg ccatcgacgc attggagcgc cagacagtcg aacgcctgat
    17641 cctcacccgg cccgcggtcg aggccggcga gcgattgggc ttcctgccgg gagacctcgc
    17701 acaaaaggtc gacccgtacc tgcgccccct tttcgacgcg ctgtacgacc tgatgggttt
    17761 cgatcgcgtc cagcgcctgt tcgacaaaca aagcatcgag atcgcgccgc ttgcgtacat
    17821 gcgcggccgc acactcaacc atgcattcgt catcctggac gaggcgcaga acaccacgcc
    17881 cgaacagatg aagatgttcc tcacccgcat cggcttcggc agcaaggccg tcatcaatgg
    17941 cgacccatcg caggtcgatc tgccgaaggg ccagcgcagc ggcctgggcc atgccattcg
    18001 gcttctggat gatgtgccag gcatcgcctt cacgcagttc accagccgcg acgtcgtgcg
    18061 ccacccgctg gtcgcccgca tcgtcgacgc cta
//
LOCUS       DASTNK010000165         6168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1453738, whole genome shotgun sequence.
ACCESSION   DASTNK010000165 DASTNK010000000
VERSION     DASTNK010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453738"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFR20_12150"
     CDS             <1..217
                     /locus_tag="VFR20_12150"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158930.1"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU0231316.1"
                     /translation="LAVRTCEERGCDLADLPLEELRAFHPSIDADVYEVLTLEGSVAS
                     RRHIGGTAPERVKLEAERILAETADQT"
     gene            complement(257..1582)
                     /gene="rlmD"
                     /locus_tag="VFR20_12155"
     CDS             complement(257..1582)
                     /gene="rlmD"
                     /locus_tag="VFR20_12155"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU0231317.1"
                     /translation="MAESFDIESLDLEARGVARRDGKVVFVEGALPGERVLARVTRRR
                     SSFDVAQLERVVRPSSQRTAPPCPNFGVCGGCAMQHLDPAAQVAVKQRALEDSLWHIG
                     KVRPERILPALHGPTWGYRHRARLSVRLVPKKGGVLVGFRERHSSYVADMTECLVLPP
                     YVSALLRPLRTLVGALSSPNRIPQIEVAAGDDTVALLMRHLEPLSDADLRLLAAFGVE
                     HHVSWWLQPGGFESVHPLRPEDEHALAYTLPEFGLRMPFRPTDFTQVNYAINRALVSK
                     ALQLLDVRETDRVADMFCGLGNFSLPLALRAREVVGVEGSEILTRRAQSTAAACGLAE
                     KTRFETRNLFEADTEWLRGLGAFDRMLFDPPREGALALSSALSSIPAHERPRRIVYVS
                     CNPATLARDAGILVHAAGYSMTSAGVINMFPHTGHVESIAVFDATPPTA"
     gene            complement(1710..2339)
                     /locus_tag="VFR20_12160"
     CDS             complement(1710..2339)
                     /locus_tag="VFR20_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231318.1"
                     /translation="MQRTRSAFAVKMSALALAIAFQGAAFAAQPATGQAATPAVATES
                     AVPVHPGHAGHHKHWRHHHQRAAMWVPGYGPVSQKEVSALKLDDTQAQLLKSAQSAQA
                     TLRQQRHDQMKQAWQARAKALESGKVDPHQAVAQREQVMQANHEARANVQKQWLALWD
                     ALKPEQQQDLAKVFKERAQKHAAWREKRAHHHEDKMHSQKHAEPAASAS"
     gene            complement(2499..3278)
                     /locus_tag="VFR20_12165"
     CDS             complement(2499..3278)
                     /locus_tag="VFR20_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU0231319.1"
                     /translation="MTPTLVFDLETLPDVVGLRRLNPGSEGLSDDEVVEAAQAARRES
                     HGTDFLPLHLQQVAVVGCVFRDDQGFRVKTLGKADDPEPMLLAGFFKTIEHYTPRLVS
                     WNGSGFDLPVLHYRSLIHSVPAPRYWDTGDDDRDFKYNNYISRYHNRHIDLMDLLAKY
                     NGRANAPLDELAKLCGFPGKLGMDGSQVWRAWREGRADEVRAYCETDVVNTWLVYCRF
                     RLLKGELDREAYEAEVRLVRDTLQASGAPHWREYLAAWDAG"
     gene            complement(3275..3922)
                     /locus_tag="VFR20_12170"
     CDS             complement(3275..3922)
                     /locus_tag="VFR20_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEU0231320.1"
                     /translation="MSGVSSAPQSGGNTYVVKPGDTLYKIASEHNMDVSELERLNDIT
                     DPKQLKIGATLRLNGSVKAPRRAEPQAPVALPVTPVAPVAPVTAPKASDAGLISWAWP
                     AQGKIIQSFNANTKGIDIAGNPGDPVLAAADGEVMYAGNGVRGLGNLILIKHSNGFIT
                     AYAHNKELLVKTGEHVRKGTKIATLGQTDTTSPRLHFEIRRHGTPVNPLSYLPSR"
     gene            complement(4081..4899)
                     /locus_tag="VFR20_12175"
     CDS             complement(4081..4899)
                     /locus_tag="VFR20_12175"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEU0231321.1"
                     /translation="MRKPLTPSPFAKGTTNRFGRSSLGHGYSASNSNARVVPPNRPAS
                     ASKLAADTSTMVNLGLNSERSRGMMIQRLREQGIRDERVLAALMAVPRHLFVDQGLAS
                     RAYEDAALPIGYGQTISQPWVVARMLSVVSQNRDLGKVLEIGAGCGYQAAVLAQLATE
                     VHSIERVRGLYELARGHLRSLKLISRVRLSFGDGMKGLPGVAPFDAIVVAAAGIEIPQ
                     PLLDQLAPGGRLIAPQGAASMQRLILVERVGPASWHREELDAVRFVPLRAGTQP"
     gene            complement(4884..5642)
                     /gene="surE"
                     /locus_tag="VFR20_12180"
     CDS             complement(4884..5642)
                     /gene="surE"
                     /locus_tag="VFR20_12180"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEU0231322.1"
                     /translation="MRILVSNDDGYNALGLEALVRALSGLAELVVVAPETNCSGASNS
                     LTLNRPLFVRSATNGFRYVNGTPSDCVHVALTGLLDFRPDLVVSGINNGANMGDDTLY
                     SGTVAAATEGYLFGIPSIAFSLTNKNWEHLDTAADVARRIVRQHLANPPGDISLLNVN
                     IPSVAADALQGMRITRLGKRHASEPVIKSSTPYGEPVYWIGPVGRASDSAADTDFGAV
                     EQHAVSITPLRLDLTHYDQVRSMSGWVESLCANP"
     gene            5764..>6168
                     /gene="plsY"
                     /locus_tag="VFR20_12185"
     CDS             5764..>6168
                     /gene="plsY"
                     /locus_tag="VFR20_12185"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU0231323.1"
                     /translation="MSSATYYAVSLFSVLIAYVLGSISFAVVVSRLMGLQDPRTFGSG
                     NPGATNVLRTGNKLAAGLTLLGDAAKGWFAVWLALRLAARFDLPDLVVGLSAIAAFLG
                     HVYPIFLRFKGGKGVATALGVLLALQPWLALAT"
BASE COUNT         1120 a         1943 c         1989 g         1116 t
ORIGIN      
        1 gctggctgtg cgcacctgcg aagagcgcgg gtgcgatctg gctgaccttc ccttggaaga
       61 gctgcgtgct ttccatccat ccatcgacgc ggatgtctac gaggtgctca cgctcgaggg
      121 ctcggtggcg tcgcgcaggc acattggcgg cacggcaccg gagcgggtca aactggaagc
      181 cgaacggatt ctggccgaga cggccgatca gacgtagcgc gcggcccgag taccagagta
      241 tcaaacgcca aggttatcag gcggttggcg gcgtggcgtc gaacactgcg atggattcta
      301 cgtggccggt gtgcgggaac atgttgatga cgccggcgct ggtcatgctg tagccggcgg
      361 cgtgcaccaa tatgccggcg tcgcgcgcca gcgtggcggg gttgcacgag acgtagacga
      421 ttcggcgtgg gcgttcgtgg gccgggatgc ttgataacgc gctggacagt gcaagggcgc
      481 cctcccgggg cggatcgaac agcatgcggt cgaaggcgcc cagcccgcgc aaccactcgg
      541 tatcggcctc gaacaggttg cgggtctcga accgggtttt ctccgcgagg ccacaggccg
      601 cggcagtgga ttgcgcgcgc cgggtcagga tctcgctgcc ctcgacgccg acgacttcgc
      661 gcgcgcgcag ggccagcggc aggctgaagt tgcccagccc gcagaacatg tcggccaccc
      721 ggtcggtttc gcgtacgtct agcaattgca atgccttgct gaccaacgcg cggttgatcg
      781 cataattgac ctgggtgaaa tcggtgggcc ggaacggcat gcgcaggccg aattccggca
      841 gggtataggc cagggcgtgt tcgtcttctg ggcgcaacgg gtgaacagat tcgaacccgc
      901 ccggttgcag ccaccatgag acatgatgtt ccacaccgaa cgcggcaagc agacgaagat
      961 ccgcgtcgga cagcggttcg agatggcgca tgagcaaggc caccgtatcg tcgcccgccg
     1021 cgacttctat ctggggaatg cgattgggcg acgacagcgc acccaccagc gtgcgcagcg
     1081 gccgcagcag cgccgacacg tatggcggca gcacaaggca ctcggtcatg tccgcgacgt
     1141 aactgctgtg ccgctcgcga aaccccacca gcaccccgcc cttcttgggc accagccgca
     1201 ccgacagtct ggcccggtgc cggtaccccc aggtgggccc atgcagcgcg ggcagaatcc
     1261 gttctggccg cactttgccg atatgccaga gcgagtcttc cagcgcgcgc tgcttgaccg
     1321 ccacttgggc cgcgggatca aggtgctgca tggcacagcc cccacagaca ccgaaattcg
     1381 gacagggcgg cgcggtgcgc tgcgacgagg gccgcacgac acgctcgagc tgcgccacgt
     1441 cgaaagacga cctgcggcgc gtgacgcggg ccaacacacg ctcgcctggc aacgcccctt
     1501 ccacgaaaac caccttgccg tcgcgacgtg ccacgccacg ggcctcaaga tcgagcgact
     1561 cgatgtcaaa agattcagcc atatcgttca atcaaacaga aacagacagg gccgaatttt
     1621 gacattgcgg ggcgcgtaag tcgaatcgag ccgcccaggg ccgtgcacgg gtcctcacgc
     1681 cggcacatgg ctccgaggcc ggctcggcat cacgaagcgc tggccgcagg ctcggcgtgc
     1741 ttctgcgaat gcattttgtc ctcatggtga tgggcgcgct tttcacgcca cgcggcatgt
     1801 ttctgcgccc gctctttgaa gaccttggca agatcctgct gctgttcggg tttcaaggca
     1861 tcccacagcg caagccattg cttttgaaca ttcgcacgcg cctcgtgatt cgcctgcatg
     1921 acctgctcac gttgtgcaac ggcctgatgt ggatcgacct tcccagactc tagcgccttg
     1981 gcccgggcct gccaagcctg cttcatctgg tcgtggcgct gctgacgaag cgtggcctgc
     2041 gccgactggg ccgatttcaa cagttgcgcc tgcgtgtcgt cgagcttcag ggccgacacc
     2101 tctttctggc tgaccggacc gtaaccggga acccacatgg ccgcgcgctg atggtgatgc
     2161 cgccagtgct tgtggtggcc ggcatgcccg gggtgcacgg gaaccgcgga ttccgtcgcg
     2221 acggctggcg tcgcggcctg gcccgttgcg ggttgggcgg cgaatgccgc tccctggaat
     2281 gcgatggcca gagccagagc cgacatcttg acggcgaaag cggaacgggt gcgttgcatg
     2341 atcgatacct cctgtgatta ggaggcttca ttatccgcag cgcgccattt ctggattgtt
     2401 tcagcgcacc cgggttctct ttcaatcggt ttcagcccgt ggcggccgcc cgccacgctg
     2461 tgggaggaaa cagggcctgc acactaacag gccctggatc agcccgcgtc ccaggcggcc
     2521 agatattcgc gccaatgcgg cgcgccactg gcctgcagcg tatcgcgcac caaccgaacc
     2581 tcggcctcat aggcctcccg atcgagctcg cccttgagga gcctgaagcg gcaatacacc
     2641 agccaggtgt tgaccacgtc ggtttcgcaa taggcgcgca cctcgtcggc ccggccttcg
     2701 cgccatgcgc gccagacttg gctgccatcc atgcccagct tgccgggaaa accgcagagc
     2761 ttggcgagct catccaacgg cgcattggcc cggccgttgt acttggccag caaatccatg
     2821 agatcgatgt gtcggttgtg gtagcggctg atgtaattgt tgtatttgaa gtcgcggtcg
     2881 tcgtcgcccg tatcccagta gcgcggcgcg ggcaccgagt ggatgaggct gcggtaatgc
     2941 agcaccggca gatcgaagcc cgacccgttc cagctcacca ggcgtggggt gtaatgctcg
     3001 atcgttttga agaaacccgc cagcagcatc ggttccggat cgtccgcctt gcccagcgtc
     3061 ttgacgcgaa acccttgatc gtcgcgaaac acgcagccga ccaccgccac ttgctgcaga
     3121 tgcaggggca ggaagtcggt gccgtgggat tcgcgccgcg cggcctgggc ggcctcgacc
     3181 acttcgtcgt ccgacaggcc ctcgctgcct gggttcaggc ggcgcagccc gacgacgtca
     3241 ggaagggttt cgaggtcgaa aaccagggtg ggcgtcatcg gctgggcagg tatgacaacg
     3301 gattgacagg cgtgccgtgg cggcggatct cgaagtgcag gcgcggcgag gtggtgtcgg
     3361 tctgccccag cgtggcgatc ttggtgccct tgcgaacgtg ctcgccggtc ttgaccagga
     3421 gctccttgtt gtgggcatag gcggtgatga agccgttgct gtgcttgatg agaatcaggt
     3481 tgccaaggcc ccgcaccccg ttaccggcgt acatgacctc gccatcggcc gcggccagta
     3541 ccggatcacc tggattgcca gcgatgtcga tgcccttcgt gttggcgttg aaactctgaa
     3601 tgattttgcc ctgggcaggc caagcccagc tgatgagccc cgcgtcactg gccttggggg
     3661 cggtcaccgg cgccaccggc gccacaggag tgaccggcag ggccactggt gcttgcggct
     3721 cggcgcgtcg aggcgccttc acactaccgt tcaaccgcaa ggtcgcgcca atcttgagtt
     3781 gtttgggatc cgtgatgtcg ttcagacgct cgagttccga aacatccatg ttgtgctctg
     3841 aggcgatttt gtaaagtgta tcgcccggct tgacaacata cgtgttgccg cctgactgcg
     3901 gcgcgctgga gacacccgac atattcgtga ttggcgcgcg attggtgcgt gaagcgcacc
     3961 ccgcaagcag cgccactgtg accaatgccg ggatgaacaa cagacgcgat ggccgatcga
     4021 ggttcggcag gacaaaaaac cgggatccac ccacatgcat agagatctcc tgaacttgta
     4081 ttagggttgt gtgccggccc gaagcgggac gaaacggacc gcatcgagtt cttcccgatg
     4141 ccacgaagcg gggccgacac gctcgaccag aatcagtcgt tgcatggacg ccgcgccttg
     4201 tggggcgatc aggcgccctc cgggtgcgag ttggtcgagc aacggctggg gaatttcgat
     4261 gccggccgcc gccaccacga tggcatcgaa cggcgccaca cctggcaggc ctttcatgcc
     4321 atcgccaaat gacaagcgca cacggctgat caacttgagc gatcggagat ggccacgggc
     4381 cagttcatac aacccacgaa cccgctcgat gctgtgcacc tcggtggcca actgtgccag
     4441 caccgccgcc tgataaccgc agccggcgcc gatttccagc accttgccca gatcgcgatt
     4501 ctggctgacg acggaaagca tgcgtgccac gacccatggt tgggatatgg tctggccata
     4561 cccgatgggt agggccgcgt cttcgtaggc acggctggcc aacccctgat ccacgaacaa
     4621 atggcgcggc accgccatca gcgccgcgag cacccgctca tcacggatgc cctgctcgcg
     4681 cagccgctga atcatcatgc cacgcgaacg ttcggagttc aaacccaaat tgaccattgt
     4741 tgacgtatcg gcggccagtt tggacgcgga tgctggtcga ttgggcggca ccacgcgcgc
     4801 gttgctgttg gacgcggagt agccatgccc caggctggaa cgcccgaagc ggttggtcgt
     4861 gcctttggcg aaaggcgacg gggtcagggg tttgcgcata gcgactccac ccaacccgac
     4921 atgctgcgca cctgatcgta gtgggtcagg tcgagccgca acggcgtgat cgagacggcg
     4981 tgttgctcca cagcgccgaa gtcggtatcg gcggcggaat cggacgcccg gccgaccgga
     5041 ccgatccagt agacgggctc tccgtaaggc gtgctgcttt tgataaccgg ctcggaggca
     5101 tggcgcttgc ccagccgggt gatgcgcatg ccctggaggg cgtcggcggc caccgaggga
     5161 atattcacgt tgagcaggga gatgtcaccc ggaggattgg ccagatgctg cctgacgatg
     5221 cggcgcgcca catcggcggc ggtgtccagg tgttcccagt tcttgttggt gagcgaaaac
     5281 gcaatggacg gtatgccgaa caaataccct tcggttgccg cggccacggt gcccgaatac
     5341 aacgtatcgt cacccatgtt cgcgccgttg ttgatccccg acacgacgag atccgggcgg
     5401 aaatcgagca gcccggtcag ggcgacatgg acgcaatcgg atggcgtgcc attgacataa
     5461 cgaaagccgt tggtggcgct gcgcacgaac agcggccgat tcagcgtgag ggaattcgag
     5521 gccccgctgc aattggtttc gggcgccacc acgacgagct cggccagccc gctcagggcg
     5581 cggaccaacg cttccagccc caaagcgttg taaccgtcat cgttcgacac caatatgcgc
     5641 atcacccttt ccatccgctg aagcaaccgt agcgattgta cccggtcgat atggggattt
     5701 ggcggcgggc acggtagaat cggagcccaa caagaccggt cgaaccaggg agcgcaatca
     5761 atcatgtcat cagccaccta ctacgctgtc agtttgtttt cagtcctgat agcgtatgtg
     5821 ctgggttcga tatcgttcgc cgttgtcgtc agccgcctca tgggcctcca ggacccccgg
     5881 acattcggtt ccggcaaccc gggcgcaacc aatgtgttgc gcacaggcaa caaactggcg
     5941 gcagggctca cactgctcgg agacgcggca aaaggctggt tcgcggtctg gctggccctg
     6001 cgtctggcgg cacgcttcga tcttcccgac ctggtcgtgg ggctgagcgc catcgccgcc
     6061 ttcctgggcc atgtgtaccc gatattcctg cgcttcaaag gcggtaaagg cgtcgccacg
     6121 gcgctcggcg tgctgctggc gctccagcca tggttggcgc tggccacc
//
LOCUS       DASTNK010000166        20369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1637558, whole genome shotgun sequence.
ACCESSION   DASTNK010000166 DASTNK010000000
VERSION     DASTNK010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 20369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20369
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637558"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..599
                     /locus_tag="VFR20_12190"
     CDS             180..599
                     /locus_tag="VFR20_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HEU0231324.1"
                     /translation="MKKTANFSLLAPCSCGSTAGYEACCGRWHHGMQRLMAPDAQTLM
                     RSRYTAFVLDELDYLLETWHASTRPASLERNAPGVKWLGLQLHSHARQDANHATVEFV
                     ARSRHNGQAVRLHEISRFVREDGRWFYVDGDFVQKPR"
     gene            1276..2250
                     /locus_tag="VFR20_12195"
     CDS             1276..2250
                     /locus_tag="VFR20_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0231325.1"
                     /translation="MAAKPVLRSIAVHLGLAIGALCLTGGFTPALAAYPDHPINMIVP
                     WGAGGGADLLGRTAGKIMSKSLNVSIPVINVPGATGMTGMTKLLTAPADGYTMAILIG
                     DTTALLTKPNPPFELSQVIPLAIMIQQPSGYWVNVNSPYKTWSELEKAAKTKTLKVAT
                     LGFGSADDITTSYLIHKLGLKLEEVPYSHPGMRYSSILGGNADVLYEQTGDVRSYFDA
                     GKIRPIIFFYPHPVNIPVFAKVPVSKNLGLNVTLPQFRAIVVKAGTPPDRVKMLADAL
                     KKVSETPAFAAYIKQEYADPHSYIPMGGARKFMEDWLQQAKATIERSK"
     gene            2250..2981
                     /locus_tag="VFR20_12200"
     CDS             2250..2981
                     /locus_tag="VFR20_12200"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEU0231326.1"
                     /translation="MRSSGVCPRGQQTLRAVEKDDDRRSVAVSHSPSPPAEKGGVREN
                     ATRVADGRERPGVQSDEPNGPEARTTWREILPYGISAIVAAWLYHVSDGFAESGMPGR
                     IGPDAWPKIALGLMFFVSCLGMIKVWLKMRGTLAPTRANSDMQALNPPQLYPRLVWIA
                     VLATVAYVWAWQVLGYFLSTILYSLVLMLIGRQRHPVVLLCGSVGFAVFFLFLFMKVV
                     YVALPLGERPFSQVSLALMSLLGIR"
     gene            2983..4494
                     /locus_tag="VFR20_12205"
     CDS             2983..4494
                     /locus_tag="VFR20_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEU0231327.1"
                     /translation="MELLSNLAVGFAHVFQPGDFLMLVIGIIVGMAVSIMPGLGFVMG
                     VVLALPFTYRMGIEPAIILLTAIYFSGTYGGAFTTILYRIPGEPSDVPLLWDGYVMAR
                     REGAAKPLGWALVAAFIGGLVSSVVMVVLAGPLTDIALKFSSEDYFAAIFFGLTTVVS
                     LAGKSLINAVISLCLGLLIASVGVDSTYGASRFTFGVPLLRDGIELVPILVGMYGLGE
                     VFKRLGQGLTLDRSQASGGRIKTYLPSLREIFRIRGAIVRSSVIGTLLGIVPGAGATI
                     TSFICYGTEKQYGKRKEKLGTGVPEGIVAPQIGSTASVAGHMMPMLTLGIPGSASTAV
                     ILAAFLLHGVQPGPMMLTDPSMRVVVYSIFASMFVSIIGMCLIGFFWIRIVVKVLSLP
                     QSVLNTIVVLFCLVGAYAARNSLGDTWMIVVFGALGYLFEKFDFPIAPLVLGAILGPI
                     GEQNFMRSMIVSHGSLGAFFQQPLSAALMAASFISVLYPLVKNNLALFRRRPA"
     gene            complement(4495..5250)
                     /locus_tag="VFR20_12210"
     CDS             complement(4495..5250)
                     /locus_tag="VFR20_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU0231328.1"
                     /translation="MSTQANAAAVRAFRLIDILAKAGKPLSLTEIVDEIDLPKQTVHR
                     LLKQLEGASLVARTEPHRHYECSARVRQMAMNLLMKVGPPAARRAILHGVADCVHETC
                     NLTVPGGEDVVYLDRVEINWCQRISLSPGSRVPFHCTASGKLFLSFLPRQQRERIIEQ
                     LPLRRYTDRTITDLDQLRCELQDIRKHRFSFNNEEYMAGVVAIGVPVLLGTGSACAAI
                     TIQSPLDRMPVAQLQAFLPTLRMAAEKAGRTFQ"
     gene            5465..6904
                     /locus_tag="VFR20_12215"
     CDS             5465..6904
                     /locus_tag="VFR20_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0231329.1"
                     /translation="MQAESLAAASATASAAEYAEYVSALVNRARAAMAEFADADQPRV
                     DEAVTALAWSLYKPEHARALAKRAVDTTGLGRVDSKIIKNQRKTFGTLRDLLRVKTVG
                     VIEEIPERGLVKYAKPVGVVAAITPSTNPSATPVNKAMFAIKGRNAIIIVPSPSGHAV
                     TSETVALMRAELEKIGLPADLVQTPDRRPSKALTAELMRQADLVVATGSQSNVRAAYR
                     SGTPAIGVGAGNVPVIIDESADLHDAAEKIGLSKTFDNATSCSSENSVVILDSIYDEA
                     LSALKKAGGYLVDPGVKADIERRLWIDGKLNRDVIARDAVVVADKLGLGADARGAAFF
                     MVEEAGIGPDHPLSGEKLSLVLTVYRARDFSDACRIVREILGFEGRGHSCGIHTGNPE
                     HAARLAETTDVVRVLVNQAHTMGNGGSFDNGLNFTLSMGCGTWGRNSITENLNYRNFI
                     NITHLATTIPPDEPTEQDLFGTYWDRYGK"
     gene            6913..8058
                     /locus_tag="VFR20_12220"
     CDS             6913..8058
                     /locus_tag="VFR20_12220"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU0231330.1"
                     /translation="MDFVEYQGKAILAEAGLPVPEGRHCTTPDEAANAVAALGPCAVK
                     AQVPAGKRGKAGGVRLVENAQEARVAASDILKLTIGGHAVGSLLVERRVPVAGEFYAA
                     VMNDVAACGPLVLFSLEGGMDIEEIAQDRPEAVCRIPVDIERGLSSRGVADALSGCDL
                     DNRTKQAVAAFLVRLYETYIQVDAELLEINPLARLEDGSLLALDCKLAVDDSAAYRQE
                     AIVRRRAPEHQTELEARGAEAGLKFIELEGNVGVLANGAGLTMTTMDVIDHFGAHPSN
                     FLEIGGDAYTKAEPALKLVLANPNVKSLIVNFCGAFARTDVMTEGVVKAWEALKPQVP
                     VFFSIHGTGEDEAVRILRSRLGIEAYDRMEDAIVAAVEATGSLEVAK"
     gene            8055..8915
                     /locus_tag="VFR20_12225"
     CDS             8055..8915
                     /locus_tag="VFR20_12225"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU0231331.1"
                     /translation="MILEKHHRVLVQGITGRQGRFWTQCMRDYGTNVVGGVNPKHAGE
                     RCCDVPVFATAKEAVQELGGVDCSVMFVPPAGALDAALDAIDAGIKQVVVLTEHIPAH
                     DVMRIHAAARRGGTRIIGPNTAGIVTPGAAFAGIMPAFNPRVFQPGDVGVVSRSGSLG
                     TLVCLEIVSAGRGQSAFIGVGGDPMLGTTTAEAVRRLADDARTAAIVIVGEIGGAMEE
                     DAAAVVRGISKPAVAFIAGRASPPGKKMGHAGAIVIGNRGGYQSKRRALEEVGVVVVD
                     TPAGIIQALR"
     gene            complement(9101..9823)
                     /locus_tag="VFR20_12230"
     CDS             complement(9101..9823)
                     /locus_tag="VFR20_12230"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0231332.1"
                     /translation="MSICTLRDQTVVVIGGSSGMGYAVAQGALTEGARVVIGSSNQAK
                     VDAATQRLGAGASGAVLDVRDEASVAAFFDALEAFDHLVFTAGDQAPHVPAPLSELDI
                     AAAGEGLKVRFWGALMAIKHAQPHLSAHGSITLTDGLLAHMPRKGVPLATAFAGATEH
                     LVRSLALDLAPLRVNSVCPGPVLTERLAPWPAEVINQMTERQPLPRAADPAEVAQAYL
                     YLMRGGYTTGQVLIVDGGRMLV"
     gene            complement(9972..10988)
                     /locus_tag="VFR20_12235"
     CDS             complement(9972..10988)
                     /locus_tag="VFR20_12235"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEU0231333.1"
                     /translation="MAHHRNQVVMLARRPQGKPQHNDFKLAETEIPKPADGEMLIQTL
                     YLSVDPGMRGMMTEAKSYSPGFEVGQPLTGRSVGRVVISRHPHFAAGDFVYERLPWQH
                     YSISDGSKAKKVDPDVAPLSTYLGVVGVPGFCAYFGLKEIGKPRAGETVVVSGASGAV
                     GMTAAQLAKLEDCRVVGIAGSREKVDYLRNELGLDAAINYRAVTDLRAALSDACPDGI
                     DVYFDNVGGDITQAALSLINHHARIVICGQTSQYNHPSESGRFAPELLIQRSALMQGF
                     VVYDYADRNDEAVARLGQLIRDGRLHYRENIIEGLENAPQAFIDLFDGKSFGKQLVRL
                     AHAT"
     gene            complement(11050..11943)
                     /gene="gcvA"
                     /locus_tag="VFR20_12240"
     CDS             complement(11050..11943)
                     /gene="gcvA"
                     /locus_tag="VFR20_12240"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HEU0231334.1"
                     /translation="MPNRLPPLRALQIFEAAARHLSYSRAADELALTQSAVSHQIRGL
                     EAWLNNRLFRRAGRSMLLTREGERLYARVHDGLACFADSVAELCADTRAGVLNISATP
                     SISAGWLIPRLPDFYRRHPEVDINLRATNALADFDLESIDLALRYGAGIWPGLHAEKL
                     LTVDLFPVCSPHYRNGRLPRSASELLDTTLLHYTYGTSWEEWFKSASIAPRAPLRGPR
                     FDDYVLAVRAATGRQGILLGRDTLVADELTSGRLVRPLRDQPSLRVFDYFIVYPDALE
                     LSPKVKLFRDWLLEQAARPAK"
     gene            12185..13120
                     /locus_tag="VFR20_12245"
     CDS             12185..13120
                     /locus_tag="VFR20_12245"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEU0231335.1"
                     /translation="MQPHGAIYILGSGAIGLPLAAYLTHAGRHAVAVRTSVDDVPKST
                     ITVSVDNGSNNISVVVDTISLTRLTRLDGLIVIAAKSHANHGIARMLHEKGATGPIVI
                     MQNGIGVEEPFLDASTSQIYRCVLYATSRASSGHAFAFRPVTSSPVGIARGTDAGLME
                     CVKELSTDEFPLRAEGDIQRETWKKAIINAVFNSICPLLDVDNGVFVRDAATASLARQ
                     LVRECLMVTKRLDMDLDERELMDQIAAISDRSSGQPISTLQDIRRGRQTEMQYLNLEI
                     VRVAAAMQPALPLPRIDLLGKLIVAKSLLAQNARQ"
     gene            complement(13397..14914)
                     /locus_tag="VFR20_12250"
     CDS             complement(13397..14914)
                     /locus_tag="VFR20_12250"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU0231336.1"
                     /translation="MAASAGEIKNIFVLLLENRSFDHMLGFSGITGTQAGTNPPVATS
                     LEGLTKSEHNGYLGARYVVGQPFHEPVAVDPAHEFLDVLEQLCGTQAIYERGQPYPKI
                     NNSGFVSDYARSHTKEEGGAVGNFGQIMDGFAADQLPVLNTLAKSFAVCDGWHASLPG
                     PTFPNRLFAMGASSNGLDHSPASLELLTWEGLDGFSYVNGSIFDALSTKFGSEAWRIY
                     AGNQFPLVCALKGISTFDVHSVDDLAADLKGGAYPYRLTWIEPDYGDMVSGTFRGGTS
                     QHPMDNAAGGENLIKRVYEAIKESPLWESSLLIISWDEHGGFYDHIPPGKAVAPGDTK
                     PGSTYNQYGFDFTQLGVRVPAVVISPRIPAGTIDHRAYDHSSIPKTIEKIFELPSLTK
                     RDAAANSVDTLLTLPEPRKDAPDMLPLTNTRGDGEDRPIPLPGNGSAERGNLPLFLHI
                     AMRHEFAVSDSRKRAAIVHRAQSIKTQAQAAQYLSEIDHRVTMAKRRAAAVKTGH"
     gene            15205..15840
                     /locus_tag="VFR20_12255"
     CDS             15205..15840
                     /locus_tag="VFR20_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU0231337.1"
                     /translation="MKASITPAETELDERLALAQRSALAERLKSLRQQRGLTQGQVTA
                     ISGISTPTLSKIENCLLSPTYDVLLRLAHGLNVDVAELFVNAQSHMGAGRRSIEHVDS
                     GEAHDTPLYRHRLLFSQLADKRMMPFVSRIKKHHTQGDEGWSRHEGEEFAYVLYGEIE
                     LHTEFYQPVALKAGEGFYIDSRMRHRVISLSDQDAEVLWVSTTPNLGRDPQ"
     gene            15876..16793
                     /locus_tag="VFR20_12260"
     CDS             15876..16793
                     /locus_tag="VFR20_12260"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0231338.1"
                     /translation="MFQVIIDSLTRASELALIAVGLTMVYEILRFASFAHVQFGTIGA
                     FITLATSVVLPLPPGWAILAGALVACVVTGGLGILSDRLIFSKLRNSPPAILMIASFG
                     LGMVMQYGVQAIWGAGARAYPLPLTRPWIILDGRLTPAGAAVIVAAVLSMLAFHLLLT
                     RTKLGMAMRATADNPQLTEASGIFSERIIHIVWFIGAALGALGGIMLGLVSQVEPGMG
                     FEIMVAVFAAAIVGGIGNVYGAVLGACIVAFAENIGLAINWAPATHLLGLGNANALYI
                     PVGYKTGISFVILILILLFRPRGLLGRRA"
     gene            16793..17743
                     /locus_tag="VFR20_12265"
     CDS             16793..17743
                     /locus_tag="VFR20_12265"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0231339.1"
                     /translation="MINFLVYLLTIVGIYGLLSLSLSFQYGQTGLINFGQVAFFMVGA
                     YVSSIATAILGWPVWVGFILAIVGGGVLGALMALPIGDLRQDYWAIATLAAAELIRII
                     FLNTNLGSDYVGASYGITNIPAPWRNHFTATQYGYFYLALVVVCVVAAYLLVTWLGRS
                     PFGRVLKGIRESDDVARALGKDVRSARIGSLIIGGALAGAGGSLYAHFVGFIDPRFFM
                     PLETYLVWAMVIMGGPGNNLGALVGTLLVELIYNSTRFIQVPAEYSSTVAALRMVLIG
                     VLILLVILYLPRGLVPERKRRYSRRGGPPDRRHSGERHVA"
     gene            17733..18542
                     /locus_tag="VFR20_12270"
     CDS             17733..18542
                     /locus_tag="VFR20_12270"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0231340.1"
                     /translation="MLLEVDSVSRHFGGLIALNDVSLTAEEGKITGLIGPNGSGKTTL
                     VNIMTGFLKPTSGRIRFDGAAVEGKEPYKIARLGLCRTFQITLNPQRMTVMENMLLGA
                     PQQLGERMLMAALHPGAVRREEQRNMERAWHLLEMVKLQDHANELAGGLSGGQKKLLA
                     LVQILMAHPKLILLDEPVAGVNPRLIDDIVDVIKKLRADGETFLIIEHNMTVIRELCD
                     TVCVLDMGHVLAEGPVEAVLAREDVLRAYIAAGGQIDHTPGASAAMPAPHG"
     gene            18535..19263
                     /locus_tag="VFR20_12275"
     CDS             18535..19263
                     /locus_tag="VFR20_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0231341.1"
                     /translation="MDERMGILQVEDLVAGYGDTEILHGVHLDITEGQISTIIGPNGA
                     GKSTLLKALMGYLIPRAGRVRFDGNDVTTLSTHLRVSRGIAFVPQLDNVFPSLSVEEN
                     LKMGGYTLTREVLATRIAEQYDRFPRLAERRRQRVNTMSGGERQMLALARALMTDPRL
                     LFLDEPTAALSPRIAAEVFEKIQEINRHGRTVLIVEQNAELSLRASTTGIVMADGRNA
                     FEGPASQILADEKIREAYLGAAKS"
     gene            19333..>20369
                     /locus_tag="VFR20_12280"
     CDS             19333..>20369
                     /locus_tag="VFR20_12280"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0231342.1"
                     /translation="MKKHLIHVAASAATALFLAGVAAPASAADIDIGVLVPLTGTNAI
                     QGKDISRGIQLAVDQINKGHAIPQTNGGTIEVGPKELGGKIKLIIEDTESRPASALDA
                     MRKLVNVNKVPVILGILSSGVCVPTGQFAQQSKVVEICSSSTSPELRKIGPYLFSAMG
                     LDDLMGKAIADFAREDSGAKTFASLVANNPFGVGMEIQSCKELDSKEAGACATKVRYD
                     QGKSDYNADLRRTVSQHPGAVLFTAYGADARLMLQQAYQQGIPVSKNWYADYPSLWIT
                     DIQKTPQIGEGIKGLEPGSAGPAYDAYSAAYHKAFGEAPTSVFGSYAYDSTMLVALAI
                     KKAGASTATAVR"
BASE COUNT         3924 a         6164 c         6320 g         3961 t
ORIGIN      
        1 aaccaccagc gcttgctggg gtcgatcggt gatatcccgc cggccgaagc cgaagagcgt
       61 tactatcggc accttgcagc accggctgtg aaacctgttt tactttaacc aaacagcctc
      121 cgcgattccc ggtgtgattc actgagtcac ccgccttttc cggcaagtga gtccgcctca
      181 tgaaaaaaac cgccaatttt tcgctgcttg cgccctgctc ctgtggcagt actgcgggct
      241 acgaggcttg ctgcggacgc tggcaccacg gcatgcagcg cctgatggct ccggatgcgc
      301 aaacgttgat gcgatcgcgg tacacggcat tcgtgctgga cgaactggat tacctgctgg
      361 agacctggca cgcgagcact cgcccagcca gccttgaacg taatgcgccc ggtgtaaagt
      421 ggctcgggct gcaactgcac agccatgccc gccaagatgc caaccacgcc acagtagagt
      481 ttgttgcccg atctcgtcac aatggacaag ccgtccgtct gcatgagatc agccgtttcg
      541 ttcgcgaaga cggacggtgg ttctatgtcg atggtgattt cgtccagaag ccgcggtaaa
      601 agcgccgcgg tggatccggg ctgcaatgtc gcggttaata cggctatgag gacacaagcc
      661 agcgtgacac ttacgaaaat ccagaatgcg gctgcgataa atcttgtgcg atacaaacca
      721 catcgtcgtt ccccctaagg catggtgcca tcgcgaggta gaaattaagt gtggtgtagc
      781 ctcttgggtg accaacctcg aattattcgg cgttgacggg cacccttgcg attggtcatg
      841 cctcacacga cgatggaaac cgaagattcg taatggcgca gcttgatgaa aaggccagcg
      901 aggactctgc ggcatataaa gctggattta agactagccc tgaggttagc ggtggttttc
      961 acgcgcttcc gcacaccgct tacactttcc caccacccaa aattgaaaaa cgcagatttc
     1021 gggactttgc tgctttccga ccctactaaa ctgacaagca gacggtcggg tatctcgcga
     1081 tgacgcgaga caagccggtc ggctcgccgg ctcggcgcgg acgccgcgtc cggtccgctt
     1141 tcgactgcca cgagaaaagg caacacgtgg cgttcatgaa gtaaacacgc ttaactgcgc
     1201 aaccatgcca aggagacaat catgctttcg gttgacctcg ggcgaggaat atacgccttt
     1261 gcaagaaaaa ccgggatggc ggcgaaaccc gtattgcgct ccattgccgt ccatttaggt
     1321 ctcgccattg gtgcgttgtg cctgacaggc ggattcaccc cggcactggc cgcctatcca
     1381 gaccacccca tcaacatgat cgtgccgtgg ggcgcggggg gcggcgccga cctgcttggc
     1441 cgcacggccg gcaagatcat gtcgaaatcc ctgaacgtat ccatcccggt gatcaatgtt
     1501 ccgggcgcca cggggatgac gggcatgacg aagttgctca cggcgccagc cgatggctat
     1561 acgatggcta ttcttatcgg cgataccacc gcgctgctga cgaagccgaa ccccccgttc
     1621 gagctcagcc aggtcatacc gctggccatc atgattcaac agccctcggg gtattgggtc
     1681 aatgtgaaca gcccctacaa gacctggagt gaactggaaa aggcggcaaa aaccaagacg
     1741 ctgaaggtcg ccacactggg ttttggcagc gccgacgaca tcacgaccag ctatctgatt
     1801 cacaagctgg ggctgaaact ggaagaagtg ccgtactccc atccgggcat gcggtacagc
     1861 tccatcctgg gcggcaatgc ggatgtcctg tatgaacaga ccggtgacgt gcgaagctat
     1921 ttcgatgccg gcaagatccg cccgataatc tttttctacc cgcatccggt caacatcccg
     1981 gtgtttgcca aggttccggt ctcgaaaaat ctggggttga atgtcacgct gccgcagttc
     2041 cgcgccatcg tcgtgaaggc gggcacaccg ccggaccgtg tcaagatgct ggccgacgcg
     2101 ctcaagaagg tatctgaaac ccccgcgttc gcggcctata tcaagcagga gtacgcggac
     2161 ccccacagct acattcccat gggcggggcc aggaagttca tggaagactg gctgcaacag
     2221 gcgaaggcca cgattgaacg gagcaagtaa tgcggtcgtc aggcgtttgt ccccgggggc
     2281 aacagacgct gcgcgccgtc gaaaaggacg atgaccggcg ctccgtggcg gtcagccact
     2341 cgccctcccc cccggcggaa aaagggggcg tccgggaaaa cgcgacgcgt gtcgccgacg
     2401 gccgggaacg gccgggtgtg cagtccgacg agccgaatgg gcctgaagcc cgcaccacat
     2461 ggcgggagat acttccctac ggtatcagcg cgatcgtggc ggcatggctc taccatgtat
     2521 cggacggctt tgcggagtcc ggcatgccgg gccgcatcgg cccggacgcc tggccgaaga
     2581 tcgcgcttgg cctgatgttc ttcgtgtcgt gcctgggcat gatcaaggtg tggctcaaga
     2641 tgcgcgggac gctggcaccc acgcgggcca actcggacat gcaggcgctg aacccgccgc
     2701 agctttatcc ccgcctcgtg tggatcgccg ttctcgcgac ggtggcctac gtctgggcct
     2761 ggcaggtgct gggttatttc ctgtcgacga tcctctacag cctcgtcctc atgttgatcg
     2821 ggcgccagcg ccatcccgtc gtcctgcttt gcggctcggt cgggtttgcc gtgttctttc
     2881 tgttcctgtt catgaaggtt gtctacgtgg cattgcctct tggcgaaaga ccgttttcgc
     2941 aggtgtcgct ggcgttgatg tcgctcctgg ggatccgatg acatggaact gctgagcaat
     3001 ctggccgtcg ggttcgcgca cgttttccag ccgggcgatt tcctgatgct ggtcatcggg
     3061 atcatcgtgg gcatggccgt gtccatcatg cctgggctgg ggttcgtcat gggggtcgtc
     3121 ctggccctgc cgttcaccta cagaatgggc atagagcccg ccatcatcct gttgacggcg
     3181 atctattttt cggggacata cggcggcgca ttcaccacca ttctgtaccg catcccgggt
     3241 gaaccgagcg acgtgccact gctctgggat ggctacgtga tggcgcgccg cgagggggcg
     3301 gccaagccgc tcgggtgggc gctggtcgcc gccttcatcg gcgggctggt ctcgtccgtc
     3361 gtgatggtcg tgctggcggg gccgctgacg gacatcgcgc tgaaattttc gtcggaagat
     3421 tatttcgccg cgatcttctt cgggttgacg acggttgtgt cgctggccgg caaatcgctg
     3481 atcaacgccg tcatcagcct gtgtctgggg ctgttgatcg cctcggtggg cgtcgacagc
     3541 acctatggcg cgagccgctt cacgtttggc gttccgctat tgcgcgacgg catagagctc
     3601 gtgccgatcc tggtggggat gtatggcctg ggcgaggtgt tcaagcgtct tggacagggg
     3661 ctgacgctgg accggagcca ggcatcgggg ggcaggatca agacctatct tcccagcctg
     3721 cgagagattt tccggatcag gggggcgatc gtgcgcagca gtgtcatagg caccttgctt
     3781 ggcatcgttc cgggcgcggg cgccacgatc acgtcgttca tctgctatgg caccgaaaag
     3841 cagtatggca agcgcaagga gaagctgggg accggcgtgc cggaggggat tgtcgcgccg
     3901 cagatcggtt ccacggcttc cgtcgcgggc catatgatgc ccatgttgac gctgggcata
     3961 ccgggcagcg cctccaccgc ggtcattctc gcggcatttc tgctgcacgg cgtgcagccc
     4021 ggccccatga tgctgacaga cccgtcgatg cgtgtcgtgg tctattcgat tttcgcgtcg
     4081 atgttcgtca gtatcatcgg catgtgcctg atcggttttt tctggatccg gatcgtggtg
     4141 aaggtgctga gcctgccgca gtcggtcctg aacaccatcg tggtgctgtt ctgcctcgtc
     4201 ggcgcctatg ccgcccggaa cagcctgggc gacacctgga tgatcgtggt ttttggcgcg
     4261 ctgggctatt tgttcgagaa gttcgatttc cccatcgcgc cgctggtgct gggcgccatt
     4321 cttggcccga taggcgagca gaacttcatg cgcagcatga tcgtttcgca cggtagcctg
     4381 ggcgccttct tccagcaacc gttgagcgcc gcactcatgg cggcgtcatt catctcggtg
     4441 ctgtatccgc tggtgaagaa caatcttgcg ctgttccggc gcaggccggc gtgatcactg
     4501 gaatgtcctg ccggcctttt ccgccgccat gcgcaaggtc ggcaggaacg cctgcaactg
     4561 cgccacgggc atccggtcga gcggcgactg gatggtgatg gcggcgcagg ccgagcccgt
     4621 gccaagcagc accggaacgc cgatggccac gacgcccgcc atgtactctt cattgttgaa
     4681 gctgaaccgg tgtttgcgga tgtcctgcag ttcgcagcgc agttggtcca ggtcggtgat
     4741 cgttcggtct gtgtaccggc gcagcgggag ctgttcgatg atgcgctcgc gctgctgcct
     4801 gggcaggaag ctcaggaaca gcttgccgct ggccgtgcaa tggaagggga cgcgcgaacc
     4861 ggggctcagc gagatgcgct ggcaccaatt gatttccacc cggtcaaggt agaccacgtc
     4921 ttcgccgccc ggcacggtga gattgcaggt ttcgtggacg cagtcggcca cgccgtgcag
     4981 gatggcgcgc cgggccgccg gaggccccac tttcatcagg agattcatgg ccatttgccg
     5041 cacgcgcgcc gagcactcat agtgccggtg cggttccgtg cgggccacga gcgaggcccc
     5101 ctccagttgt ttcagaaggc gatggaccgt ctgcttgggc aggtctattt catcgacgat
     5161 ttccgtcaac gatagcggtt tgcccgcctt tgccaggata tcgatcaaac ggaacgcccg
     5221 cacagcggcg gcgtttgctt gcgtggacat tgcgtgactc ctaactgggc acggcgtgga
     5281 gccgtgccaa cctatgcgac gtgacgcgac catggctttt cgttgattgt atgccagtgt
     5341 tgtcccgttt tccggaaaat gtgaggcgcc tctccagcgg gcattagaaa tctcgaaaat
     5401 cgggacaccg ctgacgcgcc gattcgctac attggccgca atggtgtgga aacaggaggt
     5461 gatcatgcaa gcagaatcgc tggctgcggc gtccgccacg gcttctgccg ctgaatacgc
     5521 ggaatatgta tcagcattgg tcaaccgggc acgcgcggcg atggcggagt tcgcggatgc
     5581 cgatcaaccg cgcgtggatg aggcggtgac cgccctggcg tggtcgctgt acaaacccga
     5641 gcatgccagg gcgctggcga agcgcgcggt cgacacgacg gggctgggcc gcgtcgacag
     5701 caagatcatc aagaaccagc gcaagacgtt cggcacgctg cgcgatctgc tgcgcgtgaa
     5761 gacggtgggg gtgatcgagg aaatccccga gcgcggcttg gtcaagtacg cgaagccggt
     5821 cggggtggtg gcggcgatca cgccttcgac gaacccctcg gccacccccg tcaacaaggc
     5881 gatgttcgcc atcaaggggc gcaatgcgat catcatcgtc ccttcgccgt cggggcacgc
     5941 ggtcacgagc gagacggtcg cgctgatgcg cgccgagctg gaaaagatcg gcctgccggc
     6001 ggatctggtc cagacccccg atcgcaggcc atccaaggcc ttgaccgcgg aactcatgcg
     6061 tcaggccgac ctggtcgtgg cgaccggatc ccaaagcaac gtgcgggccg cctatcggag
     6121 cggaacaccc gccatcggcg tgggcgcggg caatgtcccg gtgatcatcg acgaatccgc
     6181 agacctgcat gacgccgccg agaagatcgg cctctcgaag acattcgaca atgcgacctc
     6241 ctgctcgtcc gaaaactcgg tggtgatact ggactcgatc tatgacgagg ccctatccgc
     6301 cctgaagaaa gcgggggggt acctcgtcga tccgggcgtc aaggcggaca tcgaacgcag
     6361 gctgtggata gacggcaaac tcaatcgcga tgtcatcgcg cgcgatgcgg tggtcgtcgc
     6421 cgacaagctg gggttgggcg ccgatgcccg gggggcggcg ttcttcatgg tggaagaagc
     6481 cggcatcggt cccgatcatc cactatctgg agaaaagctt tcgctcgtcc tgacggtcta
     6541 tcgggcacgg gatttttccg atgcgtgccg gatcgtgcgc gagattctcg gcttcgaggg
     6601 gcggggccat tcctgcggca tccacaccgg gaatcctgag catgcggccc ggctcgcgga
     6661 gacgaccgat gtggtccggg tgctggtcaa tcaggcccat accatgggca acgggggcag
     6721 tttcgacaat ggcctgaatt tcacgctgag catggggtgc ggaacctggg gacggaacag
     6781 catcaccgag aacctgaatt accggaattt catcaacatc acccacctgg ccacgaccat
     6841 accgcccgac gagccgacgg agcaggatct cttcgggaca tactgggatc gctacggcaa
     6901 ataggaggga tcatggattt cgtcgaatat cagggcaagg cgattcttgc ggaggcgggc
     6961 ctgccggttc cggagggccg ccactgcacg acgcccgacg aggcggcaaa tgccgtcgcc
     7021 gcgcttggcc cctgcgcggt gaaggcgcag gtgccggccg gcaagcgtgg caaggcggga
     7081 ggggtccgcc tggtcgagaa cgcgcaggag gcccgcgtgg cggcatccga catcttgaag
     7141 ctgaccatcg gcggtcatgc ggtgggaagc ctgctggtcg aacggcgtgt gccggtcgcg
     7201 ggcgaattct acgctgcggt gatgaatgac gttgcggcgt gtggaccgct ggtcctgttc
     7261 tcgctggaag gcggcatgga tatcgaggag atcgcgcagg acagacctga ggccgtatgc
     7321 cgcatacccg tggatatcga acgggggctg tcgagccgcg gcgtggccga tgctttgtcc
     7381 gggtgcgatc tcgacaatag aacgaagcag gccgtggcgg cgttcctcgt gcgcttgtac
     7441 gaaacctaca ttcaggtgga cgcggagctg cttgaaatca acccgctggc ccggctcgag
     7501 gacggaagcc tgctcgcgct tgactgcaag ctggcggtcg acgattcggc ggcgtaccgg
     7561 caggaagcca tcgtgcggcg tcgggcccct gaacaccaga ctgaactgga agcgcgtggg
     7621 gcggaggcgg ggctcaagtt catcgagctc gaggggaatg tcggcgtgtt ggcgaacggc
     7681 gccggcctga cgatgacgac gatggatgtg atcgatcatt tcggcgcgca tccatccaat
     7741 tttctcgaaa ttggcggtga tgcgtacacg aaagccgagc ccgcgttgaa gctggtgctg
     7801 gccaacccga acgtgaagag tctgatcgtg aacttctgcg gcgcgttcgc gcgcacggat
     7861 gtcatgacgg agggggttgt caaggcttgg gaggccttga aaccgcaggt gcctgtgttc
     7921 ttctcgattc acggcacggg ggaggatgaa gcggtgcgca tcctcaggtc ccggctcggg
     7981 atcgaggcct acgatcggat ggaggatgcc atcgtggccg ccgtggaggc gactggttcg
     8041 ctggaggtcg ccaaatgatt ctggagaaac atcatcgcgt gctcgtccag ggcatcactg
     8101 gccggcaagg tcgtttctgg acacaatgca tgcgtgacta cggcacaaat gtcgtggggg
     8161 gcgtcaaccc gaaacatgcc ggcgaacggt gctgtgatgt gccggtgttc gccaccgcga
     8221 aggaagccgt gcaagaactg ggcggcgtgg attgctcggt catgttcgtg cccccggcgg
     8281 gggcgctgga cgcggcgctg gacgcgatcg atgccggcat caagcaggtg gtggtgctga
     8341 cggaacatat tccggcgcac gacgtcatgc gcatccacgc cgcggcgcgc cgcggcggta
     8401 cgcgcatcat cggaccgaac acggccggca tcgtcacgcc gggcgcggcc ttcgcgggca
     8461 tcatgcccgc cttcaatcca cgtgtttttc agccgggcga tgtgggggtg gtttcgcgca
     8521 gcggtagcct gggcacgctg gtgtgcctgg agatcgtcag cgcggggcgg ggccagtcgg
     8581 ccttcatcgg cgtcgggggt gacccgatgc tgggaaccac gactgccgag gctgtccgcc
     8641 gcctggcgga tgacgcgcgt accgcggcca tcgtcatcgt cggtgaaatc ggcggcgcga
     8701 tggaagagga tgcggccgcg gtcgtgcggg gcataagcaa gcccgccgtc gccttcattg
     8761 ccggccgcgc atcgccaccc gggaaaaaga tgggccatgc cggggcgatc gtgataggaa
     8821 accgcggcgg ctaccaatcg aaacggcgcg ccctggagga agtgggtgtt gtcgtcgtcg
     8881 acacgccggc cgggataatc caggctttgc gatagccgat tgtcattgtg gaacccacag
     8941 gcctggctgt ccggataggc ttgaagccgt tgctggtccc gggctccgcc tcatgaagct
     9001 tgagcgcgag atccggcgcc accggccaat caaagggttg tgttgcaatt cgctgcccag
     9061 ccgcgcttcc ggacacaaac ggagccccgt tgcgggatac tcacaccaac atgcgcccgc
     9121 catcgacaat cagtacctgg ccggttgtgt agccgccacg catcaggtag agataggcct
     9181 gcgccacctc ggccgggtcg gcggccctgg gcagtggctg acgctcggtc atctggttaa
     9241 tcacctctgc gggccatggt gccaaccgtt cggtaagcac cggccctggg cagaccgaat
     9301 tgacccgcag cggcgccaaa tccaaggcca gactgcggac caggtgttct gtcgcaccgg
     9361 cgaacgcagt cgccagcgga acgcctttcc tcggcatatg ggccagcaag ccgtcagtga
     9421 gtgtgatcga cccgtgcgcc gacagatgcg gttgagcatg cttgatcgcc atcagcgcac
     9481 cccaaaagcg caccttcaga ccttcacccg ccgccgcgat atccaattcc gacagcgggg
     9541 ccggcacgtg tggcgcctga tcccccgccg tgaacaccag atggtcgaag gcctcgaggg
     9601 cgtcgaagaa ggcggcgacg ctggcctcgt cgcggacatc caataccgcg cccgaagcgc
     9661 cggcacctaa ccgctgcgtc gcggcgtcga ccttggcttg gttgctcgaa ccgatcacta
     9721 ctcgcgcgcc ttcggtcaga gcaccctggg cgacggcgta ccccatgccg gaactgccgc
     9781 cgatgacgac tactgtttga tcacgcagtg tgcatatgga catggctgta ggtctctcga
     9841 atcagggatg cgttgaccgg gtaagacgag gaagccttac cttcacgtcg acgaaaatgt
     9901 taattgtaat cattcaagcg ggttgttcgg ggcctttgac gcggtgggca tcatgacggc
     9961 ccaagacgcc actacgtagc gtgcgcaagt cggacaagct gcttgccgaa actttttccg
    10021 tcgaacaggt cgatgaatgc ttgcggcgcg ttttccagcc cctcgatgat gttttcgcgg
    10081 tagtgcagcc tgccgtctct gatcagctga ccgagtcgag cgacggcctc gtcgttgcga
    10141 tccgcgtagt cgtacaccac gaacccctgc atcagggcgc ttctctggat cagcagctcc
    10201 ggcgcgaacc gcccactttc agaagggtgg ttgtattgcg aggtctgccc acagatgacg
    10261 atgcgcgcgt gatgattgat cagtgataga gcagcctggg tgatatcgcc tcccacgttg
    10321 tcaaagtaca cgtcgattcc atcgggacag gcgtcgctga gcgcagcgcg caagtcagtg
    10381 acagcgcggt agttgatggc cgcgtcgagc ccaagctcgt tgcgcaaata atccactttt
    10441 tcgcggctgc ccgctatgcc gaccacgcgg cagtcttcca gctttgccag ttgtgccgcc
    10501 gtcatgccga cggcacccga agcaccagaa accaccacgg tttcacccgc gcgcggcttg
    10561 ccgatctcct taagcccgaa gtaggcgcag aatcccggga cgccaaccac acccagatac
    10621 gtcgacagtg gcgcgacgtc gggatccacc ttctttgcct tgctgccgtc ggagatggag
    10681 taatgctgcc aggggaggcg ttcgtagacg aaatcgccgg cggcgaaatg cggatggcgc
    10741 gatatcacga cacggccgac agaacggccg gtgagcggtt gccccacctc gaaaccgggt
    10801 gaatacgatt tggcttcggt catcatgccg cgcatgccag gatccactga taaatacagt
    10861 gtctggatca gcatctcgcc gtcagccggc ttcgggatct cggtttcagc cagtttgaaa
    10921 tcgttgtgct gaggtttgcc ctgcggccgc ctggcgagca tgaccacttg gttgcgatgg
    10981 tgtgccatac gaaatctact ccctctgatt caaagctatt tggtttccgg ggccagcgtt
    11041 cccgccccgt cacttcgccg gcctggcagc ctgttcaagc aaccagtcgc gaaacagctt
    11101 caccttgggt gacagctcga gcgcatccgg atacacgatg aagtagtcaa agacccttag
    11161 ggatggttga tcccgcaacg gtcgaaccaa tcgcccgctg gtcaattcat cagcaaccag
    11221 tgtgtcccgg cccagcagga ttccttggcg tccggtcgcg gctcgcacgg cgagaacata
    11281 atcatcgaag cgcgggccac gcagcggcgc acgcggcgcg atgcttgcag acttgaacca
    11341 ctcttcccag gacgtgccgt aagtgtagtg cagcagcgtg gtatccagta gctccgatgc
    11401 cgaccggggt agacggccat tgcggtagtg cgggctgcac actggaaaga gatccacggt
    11461 cagcaacttt tccgcgtgga gccccggcca gatccccgca ccatagcgaa gcgcaaggtc
    11521 aatgctctcc agatcgaagt cggccagcgc gttcgtcgca cgcagattga tgtccacctc
    11581 gggatgacgt cggtaaaagt caggaagccg tggtatcaac cagccagcgg agatcgaagg
    11641 ggtggcacta atattgagga ctcccgcacg ggtgtcggca cacaactcgg ccacgctgtc
    11701 ggcaaagcac gccagcccgt catgcacacg ggcatacaga cgctcgcctt cacgcgttag
    11761 gagcatggat cgccccgctc ggcggaacag cctgttgttc aaccacgctt cgagccccct
    11821 gatttggtga ctcaccgcgc tctgagtcaa ggcgagctcg tccgcggcgc ggctgtaact
    11881 caagtgccgg gcagccgctt caaagatctg caaggcacgg agtggcggca ggcgattcgg
    11941 catccgtaaa tattacttca actcatgcct gtcattaaaa aacctcgttg gcgcccaagt
    12001 ccggcggggt atagagtcac aagggtgacg agtgcgactc atcttcgtct ggaacgtgtc
    12061 ggatgccgga gttcacggcc gtacatacaa tcatgccgtc gaactgtcgc acggcccgcg
    12121 cccggccgag gtcacggcgc ctcacagcaa atcagccgtt cacaaccatt gacgggaggg
    12181 ccacatgcaa ccacacggag cgatctacat cctgggttct ggagcgattg gcttgccgct
    12241 ggcagcgtat ctgacacatg cgggcagaca cgctgttgca gtgcggacga gcgtggacga
    12301 cgtgccaaag agcacaatta ctgtcagtgt ggacaatggc agcaacaaca tcagcgtggt
    12361 cgtcgacacg atctccctga ctcggttgac acgccttgac gggttaattg tcatagcggc
    12421 caagtcgcac gccaaccatg gaatcgcacg aatgctgcat gagaaggggg ccactggccc
    12481 catcgttatc atgcaaaacg gcatcggtgt cgaagagcct tttctcgatg cctcgacgtc
    12541 ccagatctat cggtgtgtcc tgtacgccac cagccgggcc agctccggac atgcgttcgc
    12601 tttccgcccc gtgacttctt ctccggtggg tatcgccaga ggaaccgatg caggcttgat
    12661 ggagtgtgtc aaggagctgt ccaccgacga gttcccgttg cgcgccgagg gtgacataca
    12721 acgagaaaca tggaagaagg ccatcatcaa cgccgtcttc aactctatct gccccctgct
    12781 tgacgtcgac aacggcgtgt ttgttcgaga tgccgccacg gcaagcctgg ccagacaact
    12841 ggtcagagag tgcctcatgg taacgaagcg actcgacatg gacctggacg aacgcgaact
    12901 gatggatcag atcgccgcga tcagcgatcg atccagtggt caaccaatct cgacgttgca
    12961 ggacattcga cgtggcaggc agaccgaaat gcagtacttg aacttggaga tcgtgcgcgt
    13021 cgccgcagcc atgcaacccg cgttgcccct tccgaggatc gatctgctgg gaaagttgat
    13081 cgttgcaaag tccttgctgg cccaaaacgc gcggcagtaa ctctccatac gcggcgtgga
    13141 aagccgccgt ggacaccgag agcggagcct gacgcgcgcc gatgatatcg atggcatcga
    13201 agggaatgga gatcgatcga aggccgcgtg atgactatcc gcgaatacct cggctactgc
    13261 gatcatggcc gtatctagat caaactaaag ggggtgagtg ctgttcagta cagaatccag
    13321 ccctcatcgg tttagccgcc cagtttagct gttcaatttc ttggggtcag ttcaagtgaa
    13381 ggcgcggttt ttcttttcaa tggcccgtct tcacagccgc cgcacgccgc tttgccatcg
    13441 tgacacgatg atcaatttcc gaaagatatt gagcggcctg tgcctgcgtc tttatcgatt
    13501 gcgcccgatg cacgatggca gcccgctttc gcgagtcgga aaccgcgaac tcgtggcgca
    13561 tggcgatatg gaggaacagc ggcaagttcc ccctctctgc ggatccgttg ccaggcagtg
    13621 ggatcggccg gtcttcccca tcacctctcg tgttagtcaa cgggagcata tctggcgcat
    13681 ctttcctggg ctccggaagt gtcagtaggg tgtcgaccga gttcgctgct gcatcacgtt
    13741 tagtcaagga gggcaattcg aagatttttt cgatggtctt tgggattgaa ctgtggtcgt
    13801 atgcccggtg gtcaattgtt cctgccggaa tgcggggaga gatgacgaca gcagggaccc
    13861 gaacccccaa ttgggtgaaa tcgaatccgt actggttata cgtcgaacct ggtttggtat
    13921 cgcctggggc tacggccttt cctggaggaa tatgatcgta aaatccgccg tgttcatccc
    13981 atgaaatgat caacagactg gattcccaaa gtggtgactc cttaattgct tcgtacaccc
    14041 gcttgatcaa gttttcgccg cccgctgcgt tatccatcgg atgctgggag gtgccgcccc
    14101 ggaacgttcc ggagaccatg tcaccatagt ctggttcgat ccaggtcagg cggtagggat
    14161 acgctcctcc tttgaggtca gcagccaaat cgtcgacaga atgcacgtca aatgtgctga
    14221 tgcctttcaa agcgcacacc agggggaact ggttccctgc gtaaatcctc catgcttcgc
    14281 ttccgaactt cgtgctcaga gcatcgaaaa tggaaccatt gacgtacgag aacccatcca
    14341 acccctccca ggtcagcaac tcgaggcttg cagggctgtg gtccaaccca ttggacgaag
    14401 cccccatggc aaagagtcga ttggggaaag tgggtcccgg cagagaggcg tgccagccgt
    14461 cacaaacggc gaaactcttg gccagagtgt tgagcaccgg cagttgatcg gcggcaaaac
    14521 cgtccatgat ctggccgaaa ttccccaccg cgccgccctc ttccttggtg tgtgaccggg
    14581 catagtcgct gacgaagcca ctgttgttga tcttgggata gggttgcccc ctttcgtaga
    14641 tcgcctgcgt tccgcagagc tgctcgagca cgtcgagaaa ctcgtgtgct gggtcgaccg
    14701 caactggctc gtgaaacggc tgcccgacaa cgtacctcgc accgaggtag ccattgtgct
    14761 ccgacttggt tagaccttcc aaagaggttg cgacaggtgg attcgttcct gcttgcgtgc
    14821 ccgtgatccc ggaaaatccg agcatgtgat caaaggaccg gttctccagg agcaagacga
    14881 agatattctt tatttctcca gctgatgcag ccattggatc cccctgaaag tggtcatcct
    14941 ctgctgagcc gcctgtcaga ctttgccacc aaggctttca aggcatggtg acttgttcag
    15001 aggatctcca gactaatgac aagcacataa ggcaacactt gctgtgccaa cacaagacta
    15061 gcgcgtcacg ccacctatag gcaatattct cgaaaactat tttcttatcg ggaaaatacc
    15121 tacggaattt ggaaaaattt gaactcacaa tagatcctgt tttccaccca cgtggcgctg
    15181 cagtccggcc ttgcccatca tcgcatgaaa gcaagcatca cccctgcgga aaccgagctc
    15241 gatgaacgcc tggcgctggc acagcgctcg gcgttggcgg aacgcttgaa atccctgcga
    15301 caacaacgtg ggcttaccca aggtcaggtg accgcgatca gcgggatttc cacgcccacg
    15361 ctgtccaaga tcgaaaactg cctgttgtcg ccaacctatg acgtcctgtt gcgcctggcg
    15421 cacggcctga acgtggatgt cgcggagctc ttcgtcaacg cccagtccca catgggggct
    15481 ggccgacgca gcatcgaaca cgtcgacagc ggcgaagccc acgacacgcc gctctaccgc
    15541 catcggttgc tgttctcgca actggccgac aagcgcatga tgccgtttgt gtcgcgcatc
    15601 aagaagcacc atacgcaggg tgacgaaggc tggagccgcc acgaaggcga agaattcgct
    15661 tatgtgctgt acggtgaaat tgaactgcac accgagttct accaacccgt cgcgctgaag
    15721 gcgggcgagg gtttctacat cgatagccgc atgcgccacc gagtcatcag cctaagcgac
    15781 caggacgccg aagtgctttg ggtcagcact acgccaaacc tggggcgcga cccccaataa
    15841 cctcgccctc accggacaac cacaggagga cagccttgtt ccaagtcatc atcgacagct
    15901 tgaccagggc gtccgagctg gctctgatcg ccgtgggcct gaccatggtc tacgagattc
    15961 tccggttcgc cagcttcgct catgtgcagt ttggcacgat cggggcattc atcaccctgg
    16021 cgaccagcgt ggtcctgccc ctgccgcccg gctgggcgat cctggctggg gctctcgttg
    16081 cctgtgtcgt gaccggtggg ctgggcatcc tcagcgaccg cctcatcttc tcgaagttgc
    16141 gcaatagccc gccagccatt ctgatgatcg catcgttcgg cctgggcatg gtcatgcagt
    16201 atggcgtgca ggccatttgg ggcgcgggtg cccgcgccta ccctcttccc ttgacacgcc
    16261 cctggatcat tctcgacggt cggctcacgc ccgccggcgc ggcggtcatc gttgccgccg
    16321 tgctgtccat gctggcgttc cacttgctgc tcacacgcac caagctgggc atggcgatgc
    16381 gcgcgaccgc cgacaacccg cagctcacag aggccagcgg cattttctcg gagcgcatca
    16441 tccacattgt ctggttcatc ggcgccgcgc tgggcgcatt gggcgggatc atgctgggct
    16501 tggtctcgca ggtggaaccc ggcatgggat tcgagatcat ggtcgccgtg ttcgccgcgg
    16561 ccatcgtggg tggcattggg aacgtctacg gcgcggtgct cggtgcgtgc atcgtggcat
    16621 tcgccgagaa catcggcttg gccatcaact gggcgccggc gacccatctg ctgggactgg
    16681 gcaacgcgaa tgcgctctac atccccgtcg ggtacaagac gggcatctcg ttcgtcattc
    16741 tgatcctgat cctgcttttt cgtccacgag ggctgctggg gaggcgcgcc tgatgatcaa
    16801 tttccttgtc tatctgttga ctattgtcgg catctacggc ctcttgagcc tgagcctgag
    16861 ttttcagtat ggccagacgg gcttgatcaa cttcggccag gtggcgttct tcatggtcgg
    16921 cgcctatgtc tccagcatcg cgaccgccat tctgggctgg ccggtgtggg tcggcttcat
    16981 cctggccatc gtcgggggcg gagtcctcgg cgccctgatg gccctgccca ttggtgacct
    17041 gcgccaagac tactgggcca tcgccacgct ggccgcggcc gaactcatcc gcatcatctt
    17101 cctgaacacc aacctgggca gcgactatgt gggggcgtcc tacggcatca ccaacatccc
    17161 ggccccctgg cggaaccact tcacggccac gcagtacggg tacttctatc tggcgctggt
    17221 ggtggtgtgc gtcgtggcgg cgtacctgct ggtcacctgg ctgggtcgct cgcccttcgg
    17281 gcgcgtgctc aaggggatac gggaaagcga tgacgtcgcg cgcgccctgg gcaaagacgt
    17341 gcgcagcgca cgcatcgggt ctctgatcat cggcggtgcg ctggctggcg cgggcggtag
    17401 cctgtatgcg cacttcgtgg gcttcatcga tccacgcttc ttcatgcccc tggaaaccta
    17461 tctggtgtgg gccatggtga tcatgggcgg tcccggcaac aatctcggcg cactggtcgg
    17521 gacactgctc gtcgagctga tctacaactc cacgcgcttc attcaggtgc cggccgagta
    17581 ttcgtccacc gtggcggcgc tgcgcatggt gctgatcggc gtactgatcc tcttggtcat
    17641 cctgtatttg ccgcgcggct tggtacccga gcgcaaacgc cgctacagcc gccggggcgg
    17701 gccaccggac aggcgccatt cgggagaacg ccatgttgct tgaagtcgac agcgtctccc
    17761 gccacttcgg gggtctgatc gccctgaacg acgtatcact cacggcggaa gaaggcaaaa
    17821 tcaccggcct gatcggcccg aatggatccg gcaagaccac cctggtcaac atcatgaccg
    17881 gctttctcaa gcccacctcg ggacggatcc ggttcgacgg cgccgcggtc gaaggaaagg
    17941 aaccatacaa aatcgcgcgc cttggcctat gccgcacatt ccagatcacg ctcaatcccc
    18001 agcgcatgac cgtcatggaa aacatgctgc tgggcgcccc ccagcagtta ggcgaacgga
    18061 tgctcatggc cgcgctgcac ccgggggcgg ttcgccgcga agagcaacgc aacatggaac
    18121 gcgcctggca tcttctggag atggtgaaat tgcaagatca cgccaacgag ctggcgggcg
    18181 ggttgtcggg cggccagaag aaactgcttg cgctcgtgca gatcctgatg gcccacccca
    18241 agctcatctt gcttgatgag cccgtggcgg gggtcaaccc aaggctgatc gacgatatcg
    18301 tcgacgtcat caagaaactg cgggcggacg gcgaaacctt tctcatcatc gagcacaaca
    18361 tgacggtgat ccgcgagctg tgcgacaccg tctgtgtcct cgacatggga cacgttctgg
    18421 cggaaggccc ggtagaagca gtcctggcgc gcgaagatgt cttgcgcgcc tacatcgcgg
    18481 cgggtggcca gattgaccac acgcctgggg cgtcggcagc gatgcccgca ccacatggat
    18541 gaacgcatgg gaatacttca agttgaagat ctggtggcgg gatatggcga tacggagatc
    18601 ctgcacggcg tccacctgga catcacagaa gggcaaatct ccaccatcat cggccccaac
    18661 ggggctggca aatcgacatt gctcaaggcc ttgatgggat atctgatacc ccgcgccggc
    18721 cgggtgcgtt ttgatggcaa cgatgtcacg accctgtcga cccatctccg ggtctccagg
    18781 ggaatcgcct ttgtgccaca actggataac gtgttcccat ccctttccgt cgaggagaac
    18841 ctcaagatgg gcgggtatac gctgacgcgc gaggtattgg cgacgcgcat cgccgagcag
    18901 tacgaccgct tcccccggct ggcggagcgc cgccgccagc gcgtgaacac catgagcggt
    18961 ggcgagcggc agatgctggc cctcgcccgg gcgctcatga ccgacccgcg cctgctgttc
    19021 ctggacgagc ccaccgcggc gctgtcgccg cgcatcgccg ccgaggtgtt cgagaagatc
    19081 caggaaatca accgtcacgg acgcacggtg ctgatcgttg aacagaacgc cgaactctca
    19141 ttgcgggcct ccaccaccgg aatcgtaatg gccgatgggc ggaacgcctt tgaaggtccc
    19201 gccagccaga ttctggcgga cgaaaaaatt cgtgaggctt atcttggcgc cgccaagagt
    19261 tagtgtgccg ccccgcggat ccccacgcag gcggttgttt gatcaccaag aaccagtatg
    19321 gaggtagaca ccatgaaaaa gcatctcatc cacgtcgcag cgagcgcggc aacagcgttg
    19381 ttccttgccg gcgtggccgc ccccgcctcg gccgccgaca tcgacatcgg cgtgctcgtg
    19441 ccgctcacgg gaaccaacgc catccagggc aaggacattt cgcgcggcat acaactggct
    19501 gtcgaccaga tcaacaaagg ccatgcgatc ccccagacga acggcggcac gatagaggtt
    19561 ggccccaagg agctgggcgg caagatcaag ctcatcatcg aggacaccga atcgcggccc
    19621 gcctctgcgc tggatgccat gcgcaaactg gtcaacgtca acaaggtccc cgtgatcctg
    19681 ggcattctgt ccagcggcgt ttgcgtcccc accggccagt tcgcccagca atcgaaggtc
    19741 gttgaaatct gctcgtcgtc gacctcgccc gaactgcgca agatcggccc ctatctgttc
    19801 agcgccatgg gcctggacga cctgatgggc aaggccatcg ccgatttcgc acgggaagac
    19861 tcgggggcca agaccttcgc cagcctggtg gccaacaacc cgttcggcgt gggcatggaa
    19921 atccaatcct gcaaagagct ggatagtaaa gaagcaggcg catgcgcgac aaaagtccgc
    19981 tacgaccagg gtaaatcgga ctacaacgcg gatcttcgcc gcaccgtgtc ccagcacccg
    20041 ggcgcagtgc tcttcaccgc ctatggcgcg gatgcccgcc tgatgctgca gcaagcctac
    20101 cagcaaggga taccggtgtc caagaactgg tatgccgact acccctcgct ctggatcacc
    20161 gacattcaga agacacccca gattggcgaa gggatcaaag gactggagcc cggttcggcg
    20221 ggccccgcct acgacgccta ctcggccgcg tatcacaagg ctttcggtga ggcgcccacc
    20281 agcgtgttcg gcagctacgc ctacgattcg accatgctgg tggccctggc catcaagaag
    20341 gccggcgcct ccacggccac cgcggtgcg
//
LOCUS       DASTNK010000167         8864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1455627, whole genome shotgun sequence.
ACCESSION   DASTNK010000167 DASTNK010000000
VERSION     DASTNK010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455627"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFR20_12285"
     CDS             <1..248
                     /locus_tag="VFR20_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0231343.1"
                     /translation="QAQLDALGEWEASPLFNDRERAVLGYTDAMTRNVKVPAETFQAV
                     RRHFNPRELVELTATIGAYNMVSRFLEALDIHSDDAR"
     gene            399..>8864
                     /locus_tag="VFR20_12290"
     CDS             399..>8864
                     /locus_tag="VFR20_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEU0231344.1"
                     /translation="MPDFIRTLASGLAVLKVSRWFEMPAAHTRDIEEPPLRAELFSAL
                     QMERHGRVLANAHQLSGHSTSNRLLERLAGNESALIGACALLREATRSGRQITPADEW
                     MLDNFYLIEEQIRIARQHLPKNYSKELPRLEKGQSADFPRVYDIALEVISHGDGRIDT
                     EGLSRFVTAYQQVTTLTLGELWAIPIMLRLALIENLRRVAARLATSRGHRDQADKWVD
                     RMVEAVDKDPASLILQVADMARSDPPMAGAFVAELARRLHGQGPSLMLALTWLAQHLS
                     EAGLTIETLIQAENQQQAADQVSVSNSIGSLRLLTSVDWRDFVEAMSQVEQTLRCDPS
                     DTYVRMDFATRDRYRHIVERLSRQADSPEADVARAAIDLAQRGLDEFGAGDARAHVGY
                     YLVDAGLPSLEAAIDVRLSWLRRLRRFGDKVPLLCYLGPVTLLTALFCAGLLQLGAGD
                     GMAGWALAGSGVLLAISGSQLAISIVNWLVTLRETPESLPRMDFTRGVPDEYATLVVV
                     PTMLFTPRNIEDLCDALEVRYLANRDGNIRFCLLTDLADAATETTDTDQALVACACRG
                     IERLNRKYASNPEADDTDGDIFLLLHRPRQWNATEGAWIGHERKRGKLGDLNAFLCGG
                     HRNPFSMIAGDTTGLDRVRYVITLDTDTRLERDSARRFVATMAHPLNRPVIDTTTHCV
                     IRGYGILQPRVATSLAGSGASRYGNLYSDESGIDPYTRTVSDVYQDVFGEGSFTGKGI
                     YDVRAFEQALAGRLPENRILSHDLLEGCYARSGLISDVQLHEEYPTRYLDDMRRRHRW
                     IRGDWQIVSWLFSRVPDTRTRLGRAFGRRNPLSALSRWKILDNLRRSLVSSALLALVL
                     LGWSLLPAPWLWTGAALAVLFLPAILASLLDLARKPGQIRLAQHLPLALRATRQRLAQ
                     AFLTLAFLPYDAALSLDAIYRSLWRLTVSHRHLLQWVPSSQAGAGHGNRLVDSYRHMA
                     VSPVVSVAMAIILGVTDPPRLWIAVPFLWLWLIAPALSWWLSLPLPREEPRLSDAQHA
                     FLQGLARRTWAFFDAHLGPDDNWLPPDNVQEDPVNTVAHRTSPTNMGLALLATLAARD
                     FGYIPAGQCLERLDKTLAAMESLERHRGHFFNWYDTQLLTPLHPRYISTVDSGNLAGH
                     LMTLEPGLLAMADEPVIHPHLLRGIADTFDVLCDTSDAGLHAALIEFRHALPADRALP
                     GGARQVLLLLRHLTGHIDAFIHDPTVTAGSSPHEWALLLRRQCRDASDDLIALAPWSL
                     DEAGPSANAPPGLPRGIPTFRELANLDPALQEIDIHGDEARADLARRLAQASREAADR
                     IALARNLAARAVELALMDYTFLYNKTTRLLAIGYNATDGRLDPSYYDLLASEARLCSF
                     VAIAQNQLPQENWFALSRRLTLSAGEPTLLSWSGSMFEYLMPMLVMPSYPNTLLDQTC
                     HAVVRRQIEYGHQRGVPWGLSESAFYTFDASLNYQYRAFGVPGLGLKRGLSDDLVIAP
                     YASMLALMVEPGAACENLRKMATMGFDSPHGFYEAVDYTPARLPRGKSMAVVKSFMAH
                     HQGMGFLSLASVLLDQPMQRRFMANPLFQSAILLLQERIPRATVSYVNIVETSDARLA
                     ASLQELPVRVLDRARTRRPEVQLLSNGRYHVMLTNSGGGSSVWRDLAVTRWREDPTRD
                     HWGVFCYVRDLTDGEFWSTTYHPTRREPDRYEVIFSEDRAEFRRTDHGVEMHTEIVVS
                     PEDDIELRRTRITNRSRVRRTLDVTSYAEIVLAPAAADNAHPAFGKLFVQTEILEERH
                     AILGNRRPRSRDEKPPWMLHLMTTHGTADGPMSFETDRARFIGPGATPIAPRAMLSRE
                     ALSGTHGAVLDPIAAARCLITLEPGQQAVVDVVLGVADSRSGALRLIDKYQDRYLADR
                     VFELAWTHNHVVLRQLNATDADARLFSQMAGAIVYANTRLRADPRLLAQNGRQQSGLW
                     GYGISGDLPIVLLRLQSAENIGLARQLIQAHAYWRTKGLAVDLVIWNEDHASYRQQLQ
                     DDILGLIASGVEAQTIDRPGGIYVRMAEQIPDDDRILLQSVARLILSDDRGTLAEQIA
                     RRTPPESRVPPLNPKTPMRGAATIEAPRADLLLPNGLGGFSPDGREYVITTDAGRAPP
                     VPWVNVLANPMLGSVVSHNGPSYTWYENAHEFRLTPWHNDPVSDSGGEAFYLRDDGSG
                     HVWSPTPLPAPGAGPYVARHGLGYSVFEHTEQGIWSELTLFVALDTPVKYAILTIRND
                     TEETRSLSATGYVQWVLGDQAEKTALHVVTEADAGSEAILARNRFNNEFHNRTAFFDT
                     DAATRTVTGDRSEFIGRNGSLASPDALRHVRLSGRLGAALDPCAAIQVPIELVPGQTR
                     EVIFVLGALAPGQDTRHITHLRDGDNARTQLDRVRQYWRQTTDTVQVRTPDPALDVLA
                     NGWLVYQTMACRLWARSGFYQSGGAYGFRDQLQDAMALVHIDPGQVREHLLRCAAHQF
                     VEGDVQHWWHPPTDRGVRTHCSDDYLWLPLAVSRYVTVTDDAGVLDERIPFLEGRAVE
                     PHEDSYYDLPMRSGQSASLYDHCARAIEHGLRFGAHGLPLMGSCDWNDGMDKVGHQGK
                     GESVWLGFFLFKVLNDFARIAQARGDAAFAGKCREQAASLGARIEKTGWDGEWYRRAY
                     FDDGTPLGSRDNNECRIDAISQSWAVLSGAGDTTRAASAMRAVEEQLVRRGQRLIQLL
                     DPPFNGDGLDPGYIQGYVPGVRENGGQYTHAAIWTIMAFAEMGRHDTAWELLDAINPV
                     NHTRTRDEVELYKVEPYVMAADVYAQAPHTGRGGWTWYTGSAGWMYRLIVES"
BASE COUNT         1566 a         3101 c         2758 g         1439 t
ORIGIN      
        1 cccaggccca gctcgatgcg ctgggcgaat gggaagcctc acccttgttc aatgaccgcg
       61 aacgcgcggt gctgggctat accgacgcca tgacacgcaa tgtgaaggtg ccggcggaaa
      121 cattccaggc ggtgcgccgc cacttcaacc cgcgcgaact tgtcgaactc acggcaacca
      181 tcggcgccta caacatggtg tcgcgttttc tggaagcgct ggacatccac tcggacgacg
      241 cgcgctgagt aggccacggg ccggcgccac cgccgcgccg tttggatgta ccctgtacag
      301 gcaatgctcc atttgagtcg gcccgccctc cgaagcaccc gaaacgaaca cgtctccacg
      361 ggaccgcgct ctggggcgcc gccgaaaagg aagccgtatt gcctgatttc atcagaaccc
      421 tcgcgtctgg gctggccgtg ctcaaggtat cacggtggtt cgaaatgccc gcggcgcaca
      481 cgcgtgacat cgaagagccg ccgctgcggg cggaactgtt cagcgcgttg caaatggagc
      541 ggcacggccg ggtgctcgcc aacgcgcacc agctcagcgg ccacagcaca tccaatcgcc
      601 tgctcgaacg actggccggc aacgagtcgg ccctgatcgg cgcctgcgcg ctgctgcgcg
      661 aggcgacacg ctccgggcgc cagatcacgc cggccgatga atggatgctg gacaacttct
      721 acctgataga agaacaaatc cgcatcgccc gccagcactt gcccaagaac tacagcaagg
      781 aattgccaag gctggaaaaa ggccagtcgg cggattttcc gcgcgtctat gacatcgccc
      841 tcgaggtgat ctcgcacggt gacggccgga tcgacacgga gggcttgagt cgcttcgtga
      901 cggcctacca gcaagtcacc accctgacgc tgggcgagtt gtgggccatc cccatcatgc
      961 tgcgcctggc attgatcgag aatctgcggc gcgtggccgc gcgcctggcc actagccgcg
     1021 ggcaccgcga tcaagccgac aaatgggtgg acaggatggt ggaagcggtg gacaaggatc
     1081 ccgccagcct gatcctgcag gtggcagaca tggcgcgttc cgacccgccc atggcgggag
     1141 cgttcgtggc ggaactggcc cggcggctgc acggccaggg cccatcgctc atgctggcgc
     1201 tgacctggct ggcacagcac ctgtctgaag ccggactgac catcgagacg ttgatccagg
     1261 ccgagaacca gcagcaggcg gccgaccagg tctcagtcag caacagcatc ggcagcctgc
     1321 gcctgctgac gtccgtggac tggcgcgatt tcgtggaagc catgagccag gtcgaacaga
     1381 cattgcgatg cgatccgtct gatacctacg tccgcatgga cttcgcgaca cgagaccgct
     1441 accgccacat cgtcgagcgc ctcagccgtc aagccgactc gcccgaagcc gatgtcgcgc
     1501 gggccgcgat cgatctcgca caacgcggtc tggacgaatt cggagccggc gacgcgcgtg
     1561 cgcacgtggg ctactacctg gtcgatgcgg gcttgcccag cctggaagcg gccatcgacg
     1621 tacgattatc atggttgcgg cgcctgcggc ggttcggcga caaagtcccg ctcctgtgct
     1681 acctgggtcc cgtgaccctc ctcaccgcgc tgttctgcgc ggggctgttg caactgggag
     1741 ccggcgacgg catggcgggc tgggcgttgg ccggatcggg cgtgctgctg gcgatctctg
     1801 gcagtcagct ggccatctcg atcgtgaatt ggctggtgac gctacgcgaa acacccgagt
     1861 cgctgccccg catggatttc acccgggggg tccccgacga gtacgccacg ctcgtggtcg
     1921 tgccgaccat gctcttcacg ccacggaaca tcgaagacct gtgcgatgcg ctggaggtgc
     1981 ggtacctggc caatcgcgac ggcaatatcc gcttctgtct gctcaccgac ctggctgacg
     2041 cggccaccga gaccaccgat accgatcagg cactggtcgc ctgcgcctgc cgtggcatcg
     2101 aacggctcaa tcggaaatac gcctcgaacc ccgaggcgga cgacaccgat ggcgacatct
     2161 tcctgctgct gcatcggcca cgacagtgga atgccacgga aggcgcctgg atcggccacg
     2221 aacgcaagcg cggaaaactc ggcgacctga acgcattcct gtgcggcgga caccggaacc
     2281 cgttctccat gatcgcgggc gacaccaccg gcctggatcg cgtgaggtac gtgatcaccc
     2341 tggacaccga cacgcgtctg gaacgggatt cggcgagacg attcgtggcc accatggcgc
     2401 acccattgaa ccgccccgtc atcgacacca ccacacactg cgtgattcgt ggctacggca
     2461 tattgcagcc acgcgtcgcc accagcctgg cgggttccgg cgcatcgcgc tacggcaatc
     2521 tgtacagcga cgaatccggc atcgacccct acacgcgcac cgtgtccgac gtgtaccagg
     2581 acgtattcgg cgaaggctcg ttcacaggga aaggcattta cgacgtgcgg gccttcgaac
     2641 aagcactcgc cggacgcctg ccggagaacc gcatcctgag ccacgatctg ctggaaggct
     2701 gctatgcccg cagcggtctg atctccgacg tgcaattgca tgaggaatac cccacccgtt
     2761 atctcgacga tatgcgccgg cggcaccgat ggatccgcgg cgactggcag atcgtgtcct
     2821 ggttgttttc gcgcgtgccg gacacgcgca cgcggctggg ccgcgcgttc ggccgccgca
     2881 acccgctctc ggccctgtcg cgctggaaga tactggacaa cctgcgccgc agcctggtgt
     2941 cgagcgccct gcttgccctg gtactgctgg gatggtcgct gctgcccgcg ccctggctgt
     3001 ggaccggcgc cgcgctggcc gtcctgtttc tgccggccat tctggcctca ctgctggatc
     3061 tggccaggaa gcccggccag atccggctgg cgcagcacct tccgctcgcg ttgcgcgcga
     3121 cacggcaacg cctggcccag gcgttcctga ccctggcatt cctgccgtac gacgccgcgc
     3181 tgagcctgga cgctatctac cgcagcctgt ggcgcctgac cgtgtcgcat cgccatctgc
     3241 ttcaatgggt gccgtcgagc caggccggcg cggggcacgg caaccgactg gtggattcct
     3301 accgccatat ggccgtcagt ccggtggtat cggtggccat ggccatcatc ctgggcgtga
     3361 cggacccacc caggctctgg atcgccgtgc catttctgtg gctatggctg atcgccccgg
     3421 ccttgtcatg gtggctgagc ctcccgctgc cgcgcgaaga accccggctc tcggatgcgc
     3481 agcatgcatt cctccaaggc ctggcgcgaa ggacctgggc attcttcgac gcccaccttg
     3541 gcccggacga caattggctg ccgcccgaca acgttcagga agacccagtc aacacggtcg
     3601 cgcaccgcac gtcacccacc aacatggggc ttgcgctgct ggccacactg gcggcccgcg
     3661 acttcgggta cattccggcc ggccagtgcc tggaacgcct ggacaagacg ctggccgcca
     3721 tggaaagcct ggaacgccac cgtggccatt tcttcaactg gtatgacacc cagctgctca
     3781 cgccgctcca tcctcgctac atctccacgg tcgacagcgg gaacctggcc ggccacctga
     3841 tgacgctgga acccggcctg ctggcgatgg ccgacgagcc tgtcatccat ccccatctgc
     3901 tgcggggtat cgccgatacg ttcgacgtgc tgtgcgacac ctcggacgcg ggcctgcacg
     3961 ccgcgctcat cgagttccgc cacgccctgc ccgctgatcg cgcgctgcct ggcggcgcgc
     4021 gccaagtgct tctcctgctg cgccacctga ccggacacat cgacgcgttc atccacgatc
     4081 cgaccgtgac ggccggctca tcgccacacg agtgggcgct attgctgcgg cgccagtgcc
     4141 gggacgcgag cgacgacctc atcgccctgg cgccctggag tctcgacgag gcgggtccat
     4201 ccgccaacgc gccaccggga ttgccacgcg gcatccccac gttcagggaa ttggccaacc
     4261 tcgaccctgc cctgcaagag atcgacatcc acggcgatga ggcgcgggcc gatctggcac
     4321 gtcgactggc ccaggccagc cgcgaggcgg cggaccggat cgccctggcg cggaacctgg
     4381 cggcgcgtgc cgtcgaactc gcgctcatgg actacacctt cctgtacaac aagacgaccc
     4441 ggttgctcgc catcggctac aacgccaccg acggccggct cgaccccagc tactacgacc
     4501 tgctggcgtc ggaagcgcgt ctgtgcagtt tcgtcgccat cgcacagaat caattgccac
     4561 aggaaaactg gttcgccctg agccgccgcc tcaccttgtc cgcgggagag cccaccctgc
     4621 tgtcgtggag cggctcgatg ttcgagtacc tgatgcccat gctggtcatg cccagctacc
     4681 cgaacacact gctggaccag acatgccacg ccgtggtccg gcgccagatc gaatacggcc
     4741 atcaacgcgg cgtgccatgg ggcctgtccg aatcggcgtt ctacaccttc gatgccagcc
     4801 tgaactatca ataccgggcc tttggcgtgc cgggcctcgg actcaagcgg ggtctgagcg
     4861 atgacctcgt catcgcgccc tacgcgtcga tgctggcgtt gatggtcgaa cccggcgcgg
     4921 cctgtgagaa cctgcggaaa atggccacca tgggcttcga cagcccccac gggttctatg
     4981 aggcggtgga ctataccccg gcgcgtctgc cgcgcggcaa gtcgatggcg gtcgtcaagt
     5041 cattcatggc gcatcaccaa ggcatgggct ttctgtcgct ggccagtgtt ctgctcgatc
     5101 agcccatgca acggcgcttc atggccaacc cattgttcca gtccgccatc ctgctgctgc
     5161 aggaacgcat cccccgcgcc acggtttcgt acgtcaacat cgtcgaaacg tcggacgccc
     5221 gcctcgccgc cagcctgcag gaattgcctg tgcgtgtgct ggatcgcgcc cgcacgcggc
     5281 gccccgaagt ccaactgctc tccaacggac gctaccatgt catgctcacc aactccggcg
     5341 gcgggtccag cgtatggcgg gatctggccg tcacgcgctg gcgcgaggat cccacccgcg
     5401 atcactgggg cgtgttctgc tacgtgcgtg acctgaccga cggtgagttc tggtccacca
     5461 cgtatcaccc gacgcggcgg gaacccgatc gctatgaagt gattttctcc gaggatcgcg
     5521 ccgaattccg ccgcacggac cacggcgtgg agatgcatac cgagatcgtc gtttcacccg
     5581 aggacgacat cgaactgcgg cgcacccgca tcaccaaccg gtcacgcgtg cgccgcacac
     5641 tggacgtgac cagctacgcg gagatcgtgc tcgcgcccgc cgccgccgac aacgcccatc
     5701 ccgccttcgg caagctgttc gtgcagaccg agatcctgga agagcgccac gccatcctgg
     5761 gcaaccgccg accgcgctcg cgcgacgaga aaccgccctg gatgctgcat ctgatgacca
     5821 cccatggcac ggcggatggg ccgatgtcct tcgaaaccga ccgggcacgg ttcatcggcc
     5881 cgggcgccac gcccatcgcc ccgcgcgcca tgctcagccg cgaagcattg tccggcaccc
     5941 acggcgcggt gctcgacccc atcgccgcgg cacgctgcct gatcacgctc gaacccggcc
     6001 agcaggcggt ggtggatgtc gtcctgggcg tggcggactc acgctcaggc gccctgcggc
     6061 tcatcgacaa gtaccaggat cgctatctgg ccgaccgggt cttcgaactg gcctggacgc
     6121 acaaccatgt cgtgctgcgc caattgaacg ccaccgacgc cgacgcgcga ttgttcagcc
     6181 agatggccgg cgcgatcgtg tatgcgaaca cccgcttgcg cgccgacccc cgattgctgg
     6241 cccagaacgg acgccagcaa tccggactct ggggctacgg catatcgggc gacctgccca
     6301 tcgtccttct gcgcctgcaa agcgccgaga acatcgggct cgcgcgccag ttgatccagg
     6361 cccacgccta ctggcgcacc aagggcctgg cggtggacct ggtcatctgg aacgaggacc
     6421 acgccagcta tcgccagcaa ctacaagacg acatcctggg cctcatcgcc tccggcgtcg
     6481 aggcccagac catcgaccga ccgggtggaa tctatgtgcg catggccgaa cagatccccg
     6541 acgacgaccg catactattg cagtccgtcg cacgcctgat attgtcggac gatcgcggca
     6601 ccctggccga acagatcgcg cgccgcactc cgcccgaatc acgcgtcccg ccgctgaatc
     6661 ccaaaacgcc gatgcgcggc gcggcgacca tcgaggcgcc ccgcgccgac ctgcttcttc
     6721 ccaatggcct gggcggattc agcccggacg gacgcgagta cgtgatcacg accgacgccg
     6781 gccgcgcgcc gcccgtgccg tgggtgaacg tgctcgccaa tccgatgctg ggcagtgtgg
     6841 tgtcccacaa cggcccatcc tatacctggt atgaaaacgc acacgagttc cggctcaccc
     6901 cgtggcacaa cgacccggtg tcggacagcg gtggcgaggc gttctatctg cgcgacgacg
     6961 gttcgggaca tgtctggtcg cccacaccac tgcccgcccc gggcgctggc ccgtacgtcg
     7021 cgcgccacgg gctgggctac agcgtcttcg aacacaccga acaaggcatc tggtccgagc
     7081 tgaccctgtt cgtggcgctc gacacaccgg tcaagtacgc catcctgacc atccgcaacg
     7141 acaccgaaga aacccggtcg ctgtcggcca cgggctacgt tcaatgggta ctgggcgacc
     7201 aagcggagaa gaccgccctg cacgtggtca cagaggccga tgcgggcagc gaagccatcc
     7261 tggcgcgcaa ccgcttcaac aacgagttcc acaaccgcac cgcgttcttc gacaccgatg
     7321 cggcgacacg cacggtcacg ggcgatcgca gtgaattcat cggccgcaac ggatcactgg
     7381 ccagccccga cgccttgcgc catgtccgcc tgtcgggcag gctgggcgcg gcgctggacc
     7441 cctgcgccgc catccaggtg ccgatcgaac tggtgccggg ccagacacgc gaggtcatat
     7501 tcgtactggg cgcgctggcc cctggccaag acacccggca catcacgcac ctgcgcgacg
     7561 gcgacaacgc gcgcacgcaa ctcgatcggg ttcggcaata ctggcggcag accacggaca
     7621 ccgtccaggt gcgaacgccc gacccggcgc tggacgtgct cgccaacggc tggctggtgt
     7681 accagaccat ggcctgccgg ctttgggcgc gcagcggctt ttatcaatcg ggcggcgcct
     7741 acggcttccg ggatcagttg caggacgcca tggcgctggt gcacatcgac ccgggacagg
     7801 tgcgcgaaca cctgctgcgt tgcgccgccc atcagttcgt cgagggcgac gtgcagcatt
     7861 ggtggcaccc gccaaccgat cggggcgtgc gcacacactg ctccgacgat tatctgtggc
     7921 tgccgctggc ggtgtcgcgc tatgtcacgg tgaccgatga cgccggcgtg ctggacgaac
     7981 gcattccatt cctcgagggt cgggcggtgg agcctcatga ggattcctac tacgacctgc
     8041 cgatgcgatc cggccagtcc gccagtctct acgaccactg cgcacgggcc atcgaacacg
     8101 gactgagatt cggcgcgcac ggcctgccgc tcatgggttc gtgcgactgg aacgatggca
     8161 tggacaaggt cggccatcag ggcaaggggg aaagcgtctg gctgggcttc ttcctgttca
     8221 aggtgctgaa cgacttcgcg cgcatcgcgc aggcccgcgg cgacgcggca ttcgccggta
     8281 aatgccgcga gcaggcggcg agcctgggcg cgcgcattga aaaaaccggt tgggacggtg
     8341 agtggtaccg acgtgcctac ttcgacgacg gcaccccgct gggctcgcgc gacaataacg
     8401 aatgccgcat cgatgccatt tcacaaagct gggccgtgct gtccggcgcg ggcgacacca
     8461 cgcgcgcggc cagcgccatg cgggccgtcg aagagcaact cgtgcggcgc ggccagcgct
     8521 tgatccaatt gctcgatcca ccgttcaacg gtgacggcct ggaccccggc tacatccagg
     8581 gctatgtccc gggcgtgcgc gagaacggcg ggcagtacac gcatgccgcc atctggacca
     8641 tcatggcctt cgccgaaatg ggccggcacg acaccgcctg ggaactgctg gacgccatca
     8701 atccggtcaa tcacacccgc acccgcgacg aggtcgagct ctataaagtg gaaccctacg
     8761 tgatggccgc cgacgtctac gcccaggcac cgcacaccgg tcgggggggc tggacgtggt
     8821 acaccggctc cgccggctgg atgtaccggc tcatcgtgga atca
//
LOCUS       DASTNK010000168        16638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1270847, whole genome shotgun sequence.
ACCESSION   DASTNK010000168 DASTNK010000000
VERSION     DASTNK010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 16638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16638
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270847"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VFR20_12295"
     CDS             complement(<1..959)
                     /locus_tag="VFR20_12295"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HEU0231345.1"
                     /translation="MDFDRSGVKALMEITNRPELVFVRGQGSWLEDGAGKRYLDFVQG
                     WAVNCLGHCPEPVTAAINRQAGLLINPSPAFYNQPSIDLAKRLVSVSCFDRIFFANSG
                     AEANEGAIKLARKWGRLHRKGAYKIITFDHGFHGRTLATMSASGKPGWDKMFAPQVDG
                     FPKADLNDLDSVRALIDDQTVAVMLEPVQGEGGVIPATVEFLKALRKLTDEAGLLLIV
                     DEVQTGMGRTGKLFAYEHAGIVPDIMTLGKGIGAGVPLAALCAREDVSCFQPGDQGGT
                     YNGNPLMTAAGVAVFDVLTAPGFMASVNDRAKQLSEGLLALSA"
     gene            1092..1958
                     /locus_tag="VFR20_12300"
     CDS             1092..1958
                     /locus_tag="VFR20_12300"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEU0231346.1"
                     /translation="MPFAIASLHSYPVKSCAGLTHQQATITPGGLEHDRHWVIVDQYG
                     IFMTQRTYPRMALIHPRIDDGVLTLSAPGMPDITVSQPALDTPPAIPVRIWAADTLGA
                     DEGDEAAQWLSDFLRTGCRLLREHPAAHRIASPDHVDTWIDKHSDWERGFPHEHRFGF
                     ADGFPFLVTSQSSLEELNHQLVNKDAGTMTMNRFRPNIVVQGLDPYDEDYLVGMRIAG
                     LTFAFVKPCARCPIPNINPDTAAVGTEPGRTLTAHRMFPQGMLFGVNAVMAGARQARL
                     RIGDVVEPEFDF"
     gene            complement(2226..2885)
                     /locus_tag="VFR20_12305"
     CDS             complement(2226..2885)
                     /locus_tag="VFR20_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU0231347.1"
                     /translation="MNLLDLLKLAAPILLKGTGYTLIFAFSAMIGGLVLGFVIAVARI
                     VPNRFVQWPAVTYVSLMRGTPLLVQVFIVYYGLPSIGIRFEPLTAGILALSLNAGAYI
                     SESLRGAILGIGRGQWSAGYSLGLTYWQNLYYVVVPQAMRLAVPAMSNTLISLIKDTS
                     LVSVITVTELLLATKDVIATTFRPLPLYLAAAAIYWVLSMGLEHVQRRLEKYFGRSQV
                     V"
     gene            complement(3098..3913)
                     /locus_tag="VFR20_12310"
     CDS             complement(3098..3913)
                     /locus_tag="VFR20_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0231348.1"
                     /translation="MKKLSHYLRWAVAVGVTTFSALAATAPAHATDLLSTVKARGTLL
                     VGMEGTYPPFNFVNTKTGKLDGFDVDVAKMIAQKLGVKVQFVKTEWSAILAGLSAGKF
                     DVIINQVGITPQREKTFDFSIPYVASSPQLILRKDEKAHYKSFADLKGKTLGVGQGSN
                     YETLAKAQKGIIVKSYPGAPEYLSDLIDHRIDAALNDQLMTAYLVKTSHLPIRGGAIV
                     GSPKFNGIPFRKGNPKFKAALDHILEASFKDGEFAKISDKWFGIDVSKVRKAG"
     gene            complement(4086..5240)
                     /gene="dinB"
                     /locus_tag="VFR20_12315"
     CDS             complement(4086..5240)
                     /gene="dinB"
                     /locus_tag="VFR20_12315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU0231349.1"
                     /translation="MNSRARRIAHVDMDAFYASVELLRYPELRGLPVVIGGRGSHAPR
                     VGADGGREFSRLRDYVGRGVATTSTYEARALGIFSAMGIMKAAALAPDAILLPADFNQ
                     YRHYSRLFKAAVARIAPCIEDRGIDEIYVDLTAHEESADILARRIKDAVREDTGGLIC
                     SVGVSPNKLLSKIASELDKPDGLTVLAPEDLQRRIWPLPVGKVNGIGPKANAKLAGLG
                     IVTIADLAAAPPDLLQEHFGLGYARWLVDAAHGVDDRPVVTHSEPKSISRETTFERDL
                     HVVRDRAELSSWLERLCTELSGDLARKGYVGRTIGVKLRFDDFRTVTRDATLDGPIAD
                     VAAMLRAARSCLKRINLDRRIRLLGVRASGLDPADQRVAGSGSGVQLDLY"
     gene            5402..6181
                     /locus_tag="VFR20_12320"
     CDS             5402..6181
                     /locus_tag="VFR20_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0231350.1"
                     /translation="MKYLLATVVLAALPGLATAATQAPDTLNQVMQSHTLRVCSPGDY
                     KPFSFMEKDGHFEGLDVDMMDSLAATLGAKVTFVKTTWKNLMSDFTSGKCDIGAGGIS
                     VSLARQQQAFFSAPYMINGKTPLTLCKNAKKYQTIAEINQPDVRVIFNPGGSNEKFAR
                     EHLSKAHLIPYKNNLGIFDEVANGKADVFVTEAAEAEVQSHLNPKLCAVNPNKPLQYA
                     EMGYLLPEGDIRFKLYVDQWLHLSKASGAYAKLEGKWLPKK"
     gene            complement(6284..6730)
                     /locus_tag="VFR20_12325"
     CDS             complement(6284..6730)
                     /locus_tag="VFR20_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEU0231351.1"
                     /translation="MDDIDQIIIAALMDDSRRSLKALSGLCGLSAPSVSDRLRRLEER
                     GALRGYTVDVDPKAFGYQLQAIVRVRPLPGQMHAIQRLIQDTFEFTECDKVTGEDCFV
                     ARLSVRSMEQLDAILDKLADRAETNTSIVKATPVKRRLPPLVRDDA"
     gene            6993..7892
                     /locus_tag="VFR20_12330"
     CDS             6993..7892
                     /locus_tag="VFR20_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0231352.1"
                     /translation="MENSTSRGSLEMIGAMLIAGTVGWFVLMSGQPVLDVVFWRCVFG
                     AITLLVICAAMGLLTGRRLNRVQLALAVISGAAIVGNWVLLFAAYSRASIGIATTVYN
                     TQPFMFVGLGVLFLGERLTLTKASWLVLAFAGMLVITQSHQAPSYGGHDYLGGIALAL
                     GAAFLYAVAALLVKRLSGIPPHLIALIQVCTGAIMLAPFAHVTGLAIPHSGAWPLLLT
                     MGIVHTGIMYVLLYGAIQKLPTALTGALSFLYPLAAIMVDWVAFGHALQWLQWVGAAA
                     ILLAVAGMQQGWAPVRRLRRANG"
     gene            complement(7905..9479)
                     /locus_tag="VFR20_12335"
                     /pseudo
     CDS             complement(7905..9479)
                     /locus_tag="VFR20_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010089302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S1"
     gene            complement(9619..11196)
                     /locus_tag="VFR20_12340"
     CDS             complement(9619..11196)
                     /locus_tag="VFR20_12340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEU0231353.1"
                     /translation="MAHVRLSGSERAMLPGARCVGDADPGEVLRATLVLRRQGLGEFH
                     DLADRLVAGEAPAPLSRQAFSEKFGAAPADMARVARFARRHGLVVMQQDVAPSTMVLE
                     GAAACFNDAFQVTLRRYQNEAGSYRGCDGPLHLPEDLHGIVTAVVGLDDRPQARAYLR
                     FHPPFRVAPDDLQTGFLPTQIASLYDFPDSSGEGQCIALLELGGGYSADDARAYFSEI
                     GVAAPTVTVVSTDGAPHDPTGAPGGPDGEVMLDVEIAGAVAPRSHLVLYFGSNSDAGF
                     LQALNGAIHDDGNRPSVISISWGGPEPSWTPQSLRALNDALKAAAMLGITVCVAAGDS
                     GAGGGLPGGDHVDFPASSPYALACGGTRLQASSGAIERETVWNDGAQGGAGGGGVSTV
                     FGLPAWQRQLTVSTPDGVTPLARRGVPDVAGNADPATGYRVRVDGSTQIIGGTSAVAP
                     LWAALIARINASRKQSVGYVNARLYGQPSVFHDITQGGNGGFSATPGWDACTGLGSPD
                     GGKVAGVFEVVATRPVP"
     gene            11513..12148
                     /gene="yfcF"
                     /locus_tag="VFR20_12345"
     CDS             11513..12148
                     /gene="yfcF"
                     /locus_tag="VFR20_12345"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HEU0231354.1"
                     /translation="MNQTLVLYSNSTYSSPYVLSVYATLVEKGLPFELKTVALDGQQF
                     GDEYASLSLTSRVPTLVAGDFSLSESSAIVEFLEDTYPAPEFTAVLPRNREPRARARQ
                     VMAWLRSDLGVLREERSTAVIFAEKNPRPLTAAGKQAADKLLRGAGHLIDEQGNNLFG
                     AWSVADVDLAVMLNRLVANGDPVPEKFRRYVTRQWERPSMKTWWERAAEHA"
     gene            12236..12940
                     /locus_tag="VFR20_12350"
     CDS             12236..12940
                     /locus_tag="VFR20_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0231355.1"
                     /translation="MSKEKVALITAGGSGMGAAAVRRLAQDGYHVAVLSSSGKGETLA
                     KELGGVGVTGTNQSQDDVARLVKLAMDKWGRVDALVNSAGHGPRAPILDISDEAWHTG
                     MEVYFLSAVRAIRLVAPIMEKQGGGSIVNISTAWAFEPSPTFPTSAVFRAGLASFTKI
                     FADTYAPKNIRINNVLPGWIDSLPATEERRDSVPMERYGKADEIAATIAFLLSDGAGY
                     ITGQNIRVDGGLTRSV"
     gene            complement(12959..13648)
                     /locus_tag="VFR20_12355"
     CDS             complement(12959..13648)
                     /locus_tag="VFR20_12355"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU0231356.1"
                     /translation="MRTSVATAFATAAGVSLGGADATAQAATPPKPQNVISPDEAVKR
                     LVDGNKRYVTGVTRRHDFAHEREALNSGQNPYAAILSCADSRIAPEYAFDSARGDLFV
                     TRVAGNFVNDDVLGSLEYAIAVLKAPLIMVLGHEGCGAVDAAVKVATQGATYPGMIQS
                     LVAALVPSVDKVKGKAKNLVEAATEQNVRDSMVALSERSAIVHDAMGAGKLRIVGGIY
                     RLATGKVDFLA"
     gene            13971..14942
                     /locus_tag="VFR20_12360"
     CDS             13971..14942
                     /locus_tag="VFR20_12360"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU0231357.1"
                     /translation="MSSVHDDSIVIDGLIIAKWDRSIFEDMKKGGLAAASCTVSVWEG
                     FQATVNNIAEMKKLIRDNAELVTLVRNAADIRQAKQDGKTGIILSFQNAHPFEDQLGN
                     IEAFADMGVRIVQLCYNTQNLVGTGCYERDGGLSGYGREVIDEMNRCGVMVDLSHVGA
                     NTSKEAILASKRPVCYSHCLPSGLKDHPRNKSDEELKFIADHGGFVGVTMFSPFLKRG
                     IEATVHDYVEAFDYVINLVGDECVGIGSDFTQGYDKGFFDWITHDKGRYRRLTDFGTV
                     LNPEGIRTIGEFPNLTRAMEHAGWATSRIEKVLGLNWMRFYDDVWSV"
     gene            15031..15552
                     /locus_tag="VFR20_12365"
     CDS             15031..15552
                     /locus_tag="VFR20_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5943 domain-containing protein"
                     /protein_id="HEU0231358.1"
                     /translation="MQPQVPIEVDPQTGVWTTDKLPMLYVPRHFFVNNHHAVETALGR
                     EKYQEILYPAGHKSAYYWCDKESRQHGLKGMDVFEHYLKRLSQRGWGRFRFESHDAPN
                     ARALIRLDDSSFVLQQPDQSGKLCYMFAGWFAGAMDWVSDQNGVATRSASRETQCASE
                     GFDHCLFTVNPLP"
     gene            15562..>16638
                     /locus_tag="VFR20_12370"
     CDS             15562..>16638
                     /locus_tag="VFR20_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methylproline demethylase"
                     /protein_id="HEU0231359.1"
                     /translation="MRYEHLFAPMTLNKLVLRNRIVSTAHAEVYAGEGGLPGERYIRY
                     YEEKAKGGLGLAICGGSSPVSINSPQGWWKSVNLATDAVVEPLSQLAEAMHRHGAKIM
                     IQATHMGRRSAWHGEHWPHLVSPSGVREPVHRGNAKTIEIEEIQRIVRDFAKAADRVK
                     RAGMDGMEVSAAHQHLIDQFWSPRTNFRSDEYGGSLENRLRFGIEALTAIREAVGTDF
                     CVGLRMCGDEFHDDGLDHEQLKEIAHAMADTGLVDYISVVGSGADTHNTLANCMPPMA
                     LPPEPFVHLAAGIKSVVRIPVMHAQSIRDANQAERILAAGLVDLVGMTRAQIADPHMV
                     IKIRDGREDEIKQCVGANYCIDRQY"
BASE COUNT         3269 a         5556 c         4888 g         2925 t
ORIGIN      
        1 ttcgccgaca acgccagcaa cccctccgac aactgcttcg cacggtcatt caccgatgcc
       61 atgaaacccg gcgcggtcag cacatcgaac accgccactc ccgccgccgt catcaacgga
      121 ttcccgttgt acgtcccacc ctggtcaccc ggctggaaac acgacacgtc ttcgcgcgcg
      181 cacagcgccg ccagcggcac gcccgcccca atacctttgc ccagcgtcat gatgtccggc
      241 acgatacccg cgtgctcgta ggcgaacagc ttgcccgtgc gccccatccc cgtctgcacc
      301 tcatcgacaa tcagcaataa ccccgcctcg tcggtcagct tgcgcaaagc cttcaggaat
      361 tccaccgtgg cgggaatcac gcccccctca ccctgcaccg gttccagcat caccgcgacc
      421 gtctgatcgt cgatcagcgc acgcaccgaa tccagatcat tcaggtccgc cttggggaag
      481 ccatcgacct gcggcgcgaa cattttgtcc cagcccggtt tgcccgacgc cgacatcgtg
      541 gccagcgtgc ggccatggaa accgtgatcg aacgtgatga tcttgtaggc gcccttgcga
      601 tgcagccgcc cccacttgcg cgccagcttg atcgcgccct cgttggcctc ggcgccgcta
      661 ttcgcgaaga aaatgcgatc gaagcacgac acggacacca gccgtttcgc cagatcgatg
      721 gacggttggt tgtagaacgc cggcgacggg ttgatgagca gccccgcctg tcggttgatg
      781 gcggccgtga cgggctccgg gcaatgaccc aggcagttca ccgcccagcc ctgcacgaaa
      841 tccaggtagc gcttgcccgc gccgtcttcg agccacgacc cctgccctcg cacgaacacc
      901 agctccgggc ggttggtaat ttccatcagc gccttcacac ccgaccgatc aaagtccatt
      961 tcgctccccg acaaaaaagg cccccagagg gcgccaccaa aaaaagaatt ttagcgcagt
     1021 gcgggtttgg gccggcacgc cgggaaccct gcagtatgat ttccagcgga attgaatctt
     1081 tcggagccgt catgccgttc gccatcgcca gcctgcacag ctatcccgtc aaatcgtgcg
     1141 ctggactgac ccaccaacag gccaccatca cccccggtgg cctggagcac gaccggcatt
     1201 gggtcatcgt cgaccaatac ggtattttca tgacccagcg cacctacccg cgcatggcct
     1261 tgatccatcc ccggatcgat gacggtgtgc tcactctttc ggctcccggc atgcccgaca
     1321 taaccgtatc gcagccagcg ctggacacac caccggcaat acccgtgcgc atctgggccg
     1381 ccgacacgct gggtgccgat gaaggcgatg aggccgcaca gtggctcagc gatttccttc
     1441 gcaccgggtg ccgcctgctg cgcgaacacc ctgccgcaca ccgcatcgcc agccctgatc
     1501 acgtcgatac ctggatagac aagcacagtg actgggagcg cggcttcccg catgagcacc
     1561 gtttcgggtt cgcagacggt ttcccgttcc tggtgaccag ccagtcatcg ctggaggaac
     1621 ttaaccacca gttggtgaac aaggacgcgg gcaccatgac catgaaccgc ttccgcccca
     1681 acatcgtggt ccagggcctg gatccttacg atgaagatta cctggtcggc atgcgcatcg
     1741 ccggtctgac tttcgccttc gtcaaaccct gcgcgcgctg ccccattccc aacatcaacc
     1801 ccgacacggc ggcggtgggc accgagccag gcagaaccct gacggcgcac cgcatgtttc
     1861 cgcaaggcat gctgttcggc gtgaacgccg tcatggcggg cgccaggcag gcgcgattgc
     1921 gcatcgggga tgtcgtggag cctgagttcg atttctaggg cattttcgat tgaaacgcat
     1981 gccgcgatcg cgagccgtct gtcggggctt ggggcgggcc cggcgtaagg cgtgcgccgg
     2041 atctgtcacg ggaaggcggg ggctgtcggc gggcgctgtc tgagttatgc cacgcgggta
     2101 agcggggcat aacgagtttc gcccgccgcc cgcctggacg aggcagatcc ggatcgagca
     2161 cgccgtgccg gacccgcccc aagccccgac agacggcgcc tttagaatga ccaccagaga
     2221 cgtcgtcaaa ccacctgcga ccgcccgaag tatttctcca gccggcgctg cacgtgttcc
     2281 aaccccatgc tcagcaccca ataaatcgcc gcagcggcca gatacaacgg cagcggccgg
     2341 aacgtagtgg cgatcacgtc tttcgtggcc aacagcagtt ccgtgaccgt gatgaccgac
     2401 accagcgacg tgtccttgat gagactgatc aaggtgttgc tcatggccgg cacggccagg
     2461 cgcatagcct gcggcaccac cacgtaatac aaattctgcc aatacgtcag ccccaggctg
     2521 tagcccgccg accactggcc acgaccaatg cccaggatgg cgccacgcag gctttccgag
     2581 atgtacgcgc ccgcgttcag gctcaaagcc agaatgccgg ccgtgagcgg ttcgaaacgt
     2641 atgccgatgc tgggcaaccc gtagtacacg atgaacacct gcaccagcag cggcgtgccg
     2701 cgcatcaggc tcacgtatgt cacggcgggc cattgcacga agcgattcgg cacgatgcgc
     2761 gctaccgcga tcacgaaccc cagcaccagc ccgcctatca tggccgagaa tgcgaagatc
     2821 agcgtgtaac ccgttccctt caacagaatc ggggcggcaa gtttcaacag gtccagaaga
     2881 ttcatggctc atccatttgc ggcggccccg ccaggaacac ggagcggcac atctctaccg
     2941 atcgttcagg cggcggcacg ctacgaccag ccagccaaaa caacacaacc ctgcggacac
     3001 tcgtccacag ggttgcatta tcacatcgtc ggccgtgggg cggccccaag ccgcagaggc
     3061 atgcggcgca gtgactgggt caaaggttct ccggcgatca gcccgccttg cggaccttgc
     3121 tgacgtcgat gccgaaccat ttgtcagaga tcttggcgaa ttcgccatcc ttgaaggagg
     3181 cttccaggat atggtcgagc gccgccttga acttggggtt gcccttgcgg aacgggatac
     3241 cgttgaactt cggcgacccc acaatggcgc cgccgcggat gggcagatgc gaggtcttga
     3301 ccaagtaggc cgtcatgagc tggtcattca gcgccgcgtc gatgcggtgg tcgatcaagt
     3361 cagaaaggta ttccggcgca ccggggtagc tcttgacgat gatgcccttc tgcgccttgg
     3421 ccaaggtttc gtagttgctg ccctggccga cgcccagcgt cttgcccttc agatcggcga
     3481 agctcttgta gtgggccttc tcgtccttgc gcaggatgag ctgcgggctg gacgccacat
     3541 agggaatgga gaaatcaaac gttttctcgc gctggggcgt gatgccgact tgattgatga
     3601 tgacgtcgaa cttgcccgcg ctgagcccgg ccagaatggc gctccattcc gtcttgacga
     3661 actggacctt cacacccagc ttctgcgcga tcattttcgc gacgtccacg tcgaaaccat
     3721 ccagcttgcc agtcttggtg ttgacgaaat tgaacggcgg gtaggtgcct tccatgccga
     3781 ccagcagggt gccgcgcgcc ttgacggtgc tgagcagatc cgtggcgtga gccggggccg
     3841 tggccgccag ggccgaaaag gtggtgacac cgacagccac cgcccaacgc aagtagtgtg
     3901 agagtttttt catgttcttc cagttgaatg aacgaggcgc agcctgacat cgcgcttgtg
     3961 gccggacgcc gctttgccca ggcacccatg ctagcaagca ccgcgacagc ttataacgat
     4021 ttgataatta cttatatatt attttgcgaa ataacgcact gcaacatact gatatttcgt
     4081 tattttcaat aaagatcgag ctgcaccccg gagcctgatc ccgccacccg ctgatcggcc
     4141 ggatccaggc cgctggcccg cacacccagc aaccggatgc ggcgatccag gttgatgcgc
     4201 ttcaggcaac tgcgcgcggc acgcagcatg gccgcgacat cggcgatcgg cccatccagc
     4261 gtcgcatcgc gcgtgaccgt gcggaaatcg tcgaaccgca gcttcacgcc tatggtgcgc
     4321 cccacataac ccttgcgcgc caggtcgccc gacaactcag tgcataaccg ctcgagccac
     4381 gacgacagtt cggcgcggtc gcgcacgaca tgcaggtcgc gctcgaacgt ggtttcgcgg
     4441 ctgatggact tgggctcgga gtgggtgacc acgggacgat catccacgcc atgcgcggcg
     4501 tccaccagcc agcgcgcgta gcccagtccg aaatgttctt gaagcaggtc gggcggcgcg
     4561 gcggccaggt cggcaatggt gacgatgccc aggccggcga gcttggcatt ggctttgggg
     4621 ccgatgccgt tgaccttgcc caccggcagg ggccagatgc ggcgttgaag gtcttccggg
     4681 gcaagaacgg tcagcccgtc tggtttgtcg agttcggacg cgatcttgga cagcagcttg
     4741 ttgggcgaca ccccgacgga gcagatgagc ccgcccgtgt cttcccgcac ggcgtctttg
     4801 atgcgccgcg ccaggatgtc ggcggactct tcgtgcgccg tcaggtcgac atagatttca
     4861 tcgatgccgc ggtcttcgat gcacggcgcg atgcgcgcga cggcggcctt gaacaagcgt
     4921 gaatagtggc gatactgatt gaagtcggct ggcagcagga tggcgtcggg cgccaaggcc
     4981 gcggccttca tgatccccat ggccgagaaa attcccaacg cccgcgcctc gtacgtggag
     5041 gtggtcgcca ccccccggcc gacgtagtcg cgcaggcgcg aaaactcgcg cccgccatcg
     5101 gcgcccacac gtggcgcatg gctgccccgc ccgccgatga cgaccggcaa gccgcgcaat
     5161 tcgggatagc gcaacaattc aaccgacgcg tagaaggcgt ccatgtcgac atgcgcgatg
     5221 cggcgggcgc gggagttcat gcgaatactg attatttgta cagttttatt ataccgccgc
     5281 ccgcgcaaaa gttgggtaat cacgcattcc cggtttagcc gaaagcgcct ataacagcca
     5341 gcacagtggc gcgccgtccg atcatggcgc gctgtcaaag aagacaaaaa ggagtcccac
     5401 cgtgaaatac ctgctcgcca ccgtcgtcct ggcagccctg cccggcttgg ccaccgccgc
     5461 gacacaagcg cccgacacgc tcaaccaggt catgcaaagc cacaccttgc gcgtgtgcag
     5521 tcccggcgac tacaagccgt tcagcttcat ggaaaaagac ggccacttcg aaggcctgga
     5581 cgtggacatg atggattccc tggccgccac cctgggcgcc aaggtgacat tcgtcaagac
     5641 cacatggaag aatctgatga gcgacttcac ctccggaaaa tgcgacatcg gcgccggcgg
     5701 catttcagtg tcgctcgccc gccagcagca agccttcttc agcgccccct acatgatcaa
     5761 cggcaagacg ccgctcaccc tctgcaagaa cgccaaaaag taccagacca tcgccgagat
     5821 caaccagccc gatgtgcggg tcatcttcaa ccccggcggc agcaacgaga aattcgcccg
     5881 cgaacacctg tccaaggccc acctcattcc ctacaagaac aacctgggca tcttcgatga
     5941 agtcgccaac ggcaaggcgg acgtcttcgt cactgaggcc gccgaggccg aggtgcaaag
     6001 ccacctgaac cccaagctgt gcgcggtcaa cccgaacaag cccctgcaat acgcggaaat
     6061 gggctacctg ctgcccgagg gcgatatccg cttcaagctg tacgtcgacc agtggctaca
     6121 cctgagcaag gccagcggcg cctacgccaa gctggagggg aagtggctgc ccaagaagtg
     6181 atgtcttctc ggccccaagc gccaaaacgg tggccgtagc cccgagagga ttcacaacgt
     6241 ggcggacaac ccggatccat ggacgtccgc cacgcgaaac gcgttatgcg tcatcccgca
     6301 ccaacggcgg caaccgccgc ttcaccggcg tcgccttgac gatggacgta ttggtttcgg
     6361 cccgatcggc cagcttgtcc aagatggcat ccagttgctc catggagcgc acgctcaatc
     6421 gcgcaacgaa gcagtcctcg cccgtcacct tgtcgcattc ggtgaattcg aaagtgtcct
     6481 ggatcagccg ctgaatggcg tgcatctggc ccggcaatgg ccggacccgg acaatggcct
     6541 gcaactgata gccaaacgcc ttcgggtcga catccacggt atagccccgc agggcgccgc
     6601 gctcctccag ccgccgcagc cgatccgaca cactgggcgc cgacagcccg cacagccccg
     6661 acagcgcctt gagcgatcgc cgggaatcgt ccatgagcgc cgctatgatg atctggtcga
     6721 tgtcgtccat ttccccatcg ctttaagaat tctctgaaac tggtcgggag catgccgcag
     6781 aggcatgcgg cgcggtgact tgttcaacgg atccttggcc gccccccgaa gtcaaggcgc
     6841 cgcctgtcgc tcgatttgcg cagaaggcca ttaggtcaaa tcaatgaatt accttgataa
     6901 aaaaggcttt aataccaatt cacctgattt tatccatggc tcaatcgctt gaaaaggccc
     6961 acactgccat cattccaaac cggagcaaaa aaatggaaaa ctcaacgagc cgtggctcgc
     7021 tggaaatgat tggcgccatg ctcatcgcgg gcaccgtggg ctggttcgtg ttgatgtcag
     7081 gccagcccgt gctggacgtg gtgttctggc gctgcgtatt cggcgccatc acgctgctgg
     7141 tcatctgcgc ggccatgggg ctgttgacgg ggcggcggct gaaccgggtg cagttggcat
     7201 tggccgtgat cagcggcgcg gccatcgtcg gcaattgggt gctgctgttc gccgcctatt
     7261 cacgcgcgtc catcggcatc gccaccaccg tctacaacac gcagcccttc atgttcgtcg
     7321 gactgggtgt tctgttcctg ggagaacgcc tgacgctcac caaggcatct tggctggtgc
     7381 tggcttttgc gggcatgctg gtcatcaccc aatcacacca ggcacccagc tacggtgggc
     7441 acgattacct gggcggcatc gccctggcgc tgggcgcggc ctttctttat gccgtggcgg
     7501 cgctgttggt caagaggctg tccggcattc caccccatct catcgccctg atacaggtct
     7561 gcaccggcgc catcatgctc gccccgttcg cccacgtcac cgggctggcc atcccccatt
     7621 ccggagcctg gccgctgctg ctgaccatgg gcatcgtcca caccggcatc atgtacgtgc
     7681 tgttgtacgg cgccatacag aaactgccca ccgcgctcac cggcgccctg tcgttcctgt
     7741 acccactggc cgccatcatg gtggactggg tcgccttcgg gcatgcgctg caatggctgc
     7801 agtgggtggg cgcggcggcg atcctgctgg cggtcgccgg catgcagcaa gggtgggcgc
     7861 ccgtgcgccg gctgcgcaga gccaacggat aagtcgttga ttcctcactt cggcgccgct
     7921 ttgccagatg ccttgcgcgc ctgcaaagcc cggatccggg tcacggcccc gtgatccgac
     7981 gtggcggcat ccatcatggc cgccacatcc gccttcaacg cggtgcgcga tccgccatcc
     8041 agataaacca tgtgcccgga cggatagaac ttcaccgcca gattcccgcg caccgaggca
     8101 ttgtccagcg gcatgttctc cagatccatc actgtctgga agaaaggtgt cacgaaatcg
     8161 tagtagccgt tggccgacaa caccttcagg tccacattca ggctcatggt cgcggccagg
     8221 tcgcccgcgg tgtagagcac gacgttgccc gtggcgtcca cgcccttctg cgcccccgtc
     8281 gggtcggtgt gatggaagtc ccagtgctgg aatgcctggt cgttcaaatc ggtgaacgcc
     8341 gaatccgagg tgaatttcag atcgtcattc agatacgtgt tccacatggc cgtgtagacc
     8401 cccgacaccg ccgtcatggt ggggtcgttg ccgccggagt tgggcgagat gttgcgcgcg
     8461 atacccgtgt ccaggccggt gacccggccg tcgtacgcac ccagcgccag ccctttgtcc
     8521 tgcagcagac ggatcaggaa cagcgtatgg cccagctcat ccccgcccgc gatgtccaga
     8581 tgccaggccc tcagggtcgg cttgtcgatg cctgtgtact ggctgagaag ggtcatggtg
     8641 gcgtccgcca ccttgggata ggcccgcaac gcctgggtga agccgccccg cgcgaacgcg
     8701 cagaccgtgt cggcgaatgc gggtagcgtg gacggtgccg gatgcacgcc cagccttttg
     8761 tgataccagg catcggccgc agcggtcggc agcgcgccca ccggattgcc cgcctgggtg
     8821 tagtccagga tggacgactg gatggtcacg ccgttgaggt ccacgccatc ttcatgcaac
     8881 acatacgaca acaccgcgct gcgcgccgtg ccatacgact cgccaaacag gaattttggc
     8941 gaattccagc ggtcgtattc cgtgaggtag cgcttgatga actgcttgag cgacgcggca
     9001 tcctggtcca cgccccagaa atcctggttc ttccaggggg cgatggcggc cgaatacccg
     9061 gtgcccaccg ggttgatgaa caccaggtcg ctcttgtcga gcagactgtc cgcgttgtct
     9121 tccagcgtat agggcgcggg cggcgtgaag tccggcatgg acgtcttgat gcgccggggc
     9181 gcgaacgacc ccagcagcac gaagaccgac gacgaacccg gcccgccgtt gtagaagaac
     9241 gtgaccggcc tgtcgcttgg cgccgcgcca tccagcgtga acgccacata gaagatcttg
     9301 gcactgggct tcgaactgct ggcgtccacc gccaccagat ggcccgcccg ggccgtgtag
     9361 gccagcgtgc gtccacccat gcgcagcgtg tgatgcgtga ccgccgcacg ctcgctggtg
     9421 tctgtcacgg aatcattcgg accattgccg tacggcgtgg gatcgaaaca gggttgatcg
     9481 gcggccgcgc ctgttgccgc acccgtgttc ttcttgcttg tcatgatgtc gctcctgctt
     9541 gggccgcaac gaattccgtc accgcccggt gaagtcgccc attcaaattc aagcgtatga
     9601 gccaaccaac caaagcactc acggcaccgg cctcgtcgcg accacctcaa aaaccccggc
     9661 caccttccct ccatccgggc tgcccaggcc ggtgcaggca tcccacccag gcgtggcgga
     9721 aaacccgcca ttgccaccct gcgtgatgtc atgaaagacc gagggctgcc cataaaggcg
     9781 ggcgttgaca taccccacgg actgtttgcg cgatgcgttg atccgcgcga tcagcgccgc
     9841 ccatagcggc gcgaccgcgc tggtcccgcc gatgatctgc gtcgagccat cgacacgcac
     9901 ccgatacccc gtggccgggt cggcattgcc agccacgtcc ggcaccccac gccgcgccag
     9961 tggggtcacg ccgtccggtg tgctgaccgt gagctgtcgc tgccacgcgg gcaacccgaa
    10021 caccgtgctt acacctccgc cacccgcgcc accctgcgcg ccgtcgttcc agacggtctc
    10081 gcgttctatg gcgccggacg atgcctgcag ccgcgtgccg ccgcaagcca gcgcgtaggg
    10141 gctggaggcg gggaaatcga cgtggtcgcc gcctggcagc ccaccaccgg caccgctgtc
    10201 gcccgccgcc acgcacaccg tgatgcccaa catcgcggcc gccttcagcg cgtcattgag
    10261 cgcgcgcaac gactgtggcg tccacgacgg ctcgggcccg ccccaactga tggaaatgac
    10321 ggatggtcga ttcccgtcgt catggatggc gccgttgagc gcctggagaa atcccgcatc
    10381 actgtttgaa ccgaagtaca gcaccaaatg cgacctgggc gccaccgctc cggcgatctc
    10441 cacgtccagc atgacctcgc catccgggcc gcctggcgcg cccgtgggat cgtggggcgc
    10501 tccatcggtc gacaccacgg tcacggtggg cgccgccacc ccgatctcgg aaaaatacgc
    10561 ccgcgcatcg tccgcgctgt agccgccccc cagttcgagc agggcgatgc attggccttc
    10621 gccggaggaa tccggaaaat cgtacaagga cgcgatctga gtgggcagga aacctgtctg
    10681 aaggtcatca ggcgcaacgc gaaatggagg gtggaaacgt aaatacgcac gcgcctgcgg
    10741 gcgatcgtcc aggccaacca ccgcagtgac gatgccgtgc agatcctccg gcaagtgcaa
    10801 cgggccatca cagccccgat acgatcccgc ctcgttttga tagcgccgca gcgtgacttg
    10861 aaacgcgtcg ttgaaacacg ccgccgcccc ttccagcacc atcgtgctcg gggccacgtc
    10921 ctgctgcatg acgaccagcc cgtgacgacg cgcgaaccgc gccaccctgg ccatatcggc
    10981 gggtgcggca ccgaatttct cagagaatgc ctggcgcgaa agcggcgcgg gcgcctcacc
    11041 cgccacgagc ctgtcagcca agtcatggaa ttcgcccaac ccttgacgcc gcaacaccag
    11101 cgtcgcccgc agcacctcac ccgggtcggc gtccccgacg caccgggcac cggggagcat
    11161 tgcacgctcg ctaccggaaa gacggacatg tgccatgcgc caagcctccc attcgctgtg
    11221 aaacacgcat cgaacaatgc gcggaacgac cgccacgcgc ggcctgtcct caattcattt
    11281 aacgcccgca ccagctccca cacaagcgcc gcaataggtt cttacacctc ctttcacgag
    11341 ccatgagaag tgcgaccaga tgttgtttgt cgcgggttca tgacgagcag gccatatgag
    11401 tgacaggttg atccactgtc gacagcatgt gggttctcgg ttggcggtat gctattggcc
    11461 tgtaatgatg gctgcgcgcg cggcctcttt tcacgaacgg gagcctttgt ccatgaatca
    11521 gaccctggtg ctgtattcca acagcacata ctccagtccc tatgtcctgt cggtctacgc
    11581 gaccctggtc gaaaaaggcc tgcccttcga actcaagact gtcgcgctcg acggccagca
    11641 attcggcgac gagtacgcca gcctgtcgct gacatcacgc gttcccaccc tggtggccgg
    11701 tgacttttcc ctgtctgaat cgtcggccat cgtcgaattc ctggaagaca cctatccggc
    11761 gcccgaattc accgccgtcc tgccgcgcaa ccgtgaaccg cgtgctcggg cgcgccaggt
    11821 catggcctgg ctgcgaagcg acctcggcgt gctgcgcgaa gaacgctcca ccgcggtcat
    11881 cttcgctgaa aagaatcccc ggccgctgac ggcggcggga aagcaggccg ccgacaaact
    11941 gctgcgcggt gccggccatc tcatcgacga acagggcaac aacctgttcg gggcgtggtc
    12001 cgtcgccgac gtcgatctgg cggtgatgct caaccgcctc gtcgccaatg gcgatcccgt
    12061 ccccgagaaa ttccggcgct atgtcacgcg ccaatgggag cggccgtcga tgaagacctg
    12121 gtgggaacgc gcggccgagc acgcataggc gtcagcgtgg cggtcgcggc gtgtagtgag
    12181 gctcgacacc agccccctgt catcatttcc tgaaatccga cgattggagt ttccgatgtc
    12241 caaagaaaag gttgcattga tcacggcggg cggttcgggc atgggcgcgg ccgccgtgcg
    12301 caggctggcg caggacggct accacgtcgc cgtgctgtcc tcgtccggaa agggggagac
    12361 ccttgccaag gaactgggcg gcgtgggcgt caccggcacc aatcagtcgc aggatgacgt
    12421 ggcacgcctg gtcaagctgg cgatggacaa atgggggcgg gtcgatgccc tggtcaattc
    12481 cgccggccat ggcccccgcg cgccgattct cgatatttcc gacgaagcct ggcacaccgg
    12541 catggaggtc tatttcctga gcgcggtgcg cgccatccgg ctcgtcgcgc ccatcatgga
    12601 aaaacaaggc ggcggatcga tcgtcaacat ttccaccgcc tgggccttcg agccgtcgcc
    12661 aacatttccc acgtctgcgg ttttccgcgc cgggctggca agcttcacca agattttcgc
    12721 cgacacctac gcaccgaaga acatccgcat caacaacgtg ctgccgggct ggatcgacag
    12781 cctcccggca accgaagagc ggcgcgacag cgtgcccatg gaacgttacg gaaaggccga
    12841 tgaaatcgcg gccaccatcg ccttcctgct gtcggacggc gccggctaca tcaccggcca
    12901 gaatattcgt gtcgatggcg ggctgacacg gtcggtttga atgtggtgtg ccagcccctc
    12961 aagccaggaa atcgactttc ccggtggcca gccggtaaat accacccacg attcgcaatt
    13021 tccccgcccc catcgcgtca tggacgatgg cggaccgctc cgacagggca accatgctgt
    13081 cgcgcacatt ctgttcggtt gccgcttcca ccaggttctt cgctttaccc ttgaccttgt
    13141 cgacggatgg caccagggcc gccaccagtg attggatcat gcccggataa gtggccccct
    13201 gcgtcgccac cttgacggcc gcatcaaccg cgccgcaccc ttcgtgtcca agaaccatga
    13261 tcaacggcgc cttcaacacc gcgatggcat attccaggct gcccagcacg tcatcgttga
    13321 cgaaattgcc cgccacccgt gtgacgaaca ggtcgccccg ggcactgtcg aacgcatatt
    13381 cgggtgcgat gcgcgaatcc gcgcaactga gtatggccgc ataggggttc tgccctgagt
    13441 tgagcgcttc ccgttcatgc gcgaaatcat gacgccgggt cacgccggtc acgtagcgct
    13501 tgttcccgtc gacgaggcgc ttcaccgcct cgtcgggaga gatcacgttc tgcggcttgg
    13561 gcggcgtcgc cgcctgtgcc gtggcatccg cccctcccag cgatacaccg gcggccgtgg
    13621 caaatgccgt ggctacgctg gtacgcaaga ataatcgacg ggatggcgtg gctgccaccg
    13681 acgcgcagag tttcgaatca cacgaatcac acattggata ccccaaaaag gcgcgcgtgg
    13741 cgcggatgtc acgacattct tggacaaaac ctgatcagaa tcaatccgcc atgcaccatc
    13801 ggccagcctc tcctggcacc atcctcaaaa cgacaaccgg cgatccatgt cgcaattgag
    13861 ctggaagacg tctttcaaag aacattcgag catgactttt tggccaagaa ttgcatacat
    13921 gcctcatccc gatccgggcg atggcaagct ggcgcttagg agtcgacatc atgagttctg
    13981 tgcacgatga cagcatagtc atcgacggtc tgatcatcgc caaatgggac cgctccattt
    14041 tcgaagacat gaagaaaggc gggctggccg ccgccagttg caccgtctcg gtgtgggaag
    14101 gcttccaggc caccgtcaac aatatcgccg agatgaagaa gctcatccgc gacaatgccg
    14161 aactggtcac cctggtgcgc aacgccgcgg acatccggca agccaagcaa gatggcaaga
    14221 ccggcatcat cctgagcttc cagaacgcgc accctttcga agaccaactg ggcaatatcg
    14281 aggccttcgc cgacatgggc gtgcgcatcg tgcagctctg ctacaacacc cagaacctgg
    14341 tcggcaccgg ctgctacgaa cgcgacggtg gcctgtctgg atacggccgc gaggtcatag
    14401 acgaaatgaa ccgctgcggc gtcatggtcg acctgtcgca cgtgggcgcc aatacctcga
    14461 aagaagccat tctggcctcg aaaaggccgg tctgctattc ccactgcctg ccctcaggcc
    14521 tgaaagacca ccctcgcaac aagagcgacg aagaactcaa attcatcgct gatcacggcg
    14581 gtttcgtcgg ggtaaccatg ttctcgccgt ttctcaagcg cggcatagag gccactgtcc
    14641 acgactacgt cgaagccttc gactatgtca tcaacctcgt cggcgacgag tgcgtgggca
    14701 taggcagcga tttcacgcag gggtacgaca agggcttctt cgactggatc acacacgaca
    14761 agggccgcta ccgccgcctg accgacttcg gcacagtgct caatccagag ggcatacgca
    14821 ccatcggtga gttccccaac ctgacacgag ccatggaaca cgcgggctgg gccacatcac
    14881 gcatagaaaa agtgctgggc ctgaactgga tgcgtttcta cgacgacgtc tggagcgtct
    14941 aagccacccc aacgacaact ctgcataccg cctccccgga agcccctcag gcccggggat
    15001 tcctacgcac gcgcccggaa gccacgatcc atgcaacccc aagtacccat agaagtcgac
    15061 ccacaaaccg gtgtgtggac cacggacaag ctgcccatgc tgtacgtgcc ccggcatttc
    15121 ttcgtcaaca atcaccatgc cgtcgaaaca gccctgggac gggaaaaata tcaggagatc
    15181 ctgtacccgg cgggccacaa atccgcctat tactggtgcg acaaggaaag tcgccagcac
    15241 ggcctgaaag gcatggacgt gttcgaacac tacctgaagc gtctgtcgca acgcggctgg
    15301 gggcggttcc gcttcgaatc acacgatgcg cccaacgccc gcgccctcat ccgcctggac
    15361 gattcgtcat tcgtcctgca gcagccggat cagtccggca agctgtgcta catgttcgcc
    15421 ggctggttcg ccggcgccat ggattgggtc tctgaccaga atggagtcgc cacccgatcg
    15481 gcatcacggg aaacacaatg cgccagcgag ggattcgatc actgcctctt caccgtcaat
    15541 cccctgccat agggccgcgc catgcgttac gaacatcttt tcgccccgat gacgctgaac
    15601 aagctggtgt tgcgcaaccg catcgtcagc acggcccacg ccgaggtcta cgcgggggaa
    15661 ggcggcctgc cgggtgagcg ctacatccgc tactacgagg aaaaggccaa gggcggtctg
    15721 ggcctcgcca tatgcggtgg atccagcccg gtgtccatca acagcccgca gggctggtgg
    15781 aagtccgtca acctggccac ggacgctgtg gtcgaaccgc tgtcgcaact ggccgaggcc
    15841 atgcaccgcc acggtgcgaa gatcatgatc caggcaaccc atatgggccg ccgctccgcc
    15901 tggcatggcg agcattggcc acatctggtc agcccgtccg gtgtgcgcga acccgtgcac
    15961 cgtggcaacg ccaagaccat cgaaatcgaa gagatccagc gcatcgtgcg cgacttcgcc
    16021 aaggcggcgg accgcgtcaa gcgggccggc atggacggca tggaggtctc ggccgcgcac
    16081 cagcacctca tcgatcaatt ctggagcccg cgcaccaatt tccgcagtga cgagtacggc
    16141 ggctcactgg aaaaccgcct gcgtttcggt atcgaagccc tgaccgccat ccgcgaggct
    16201 gtgggaaccg atttctgcgt gggcctgcgc atgtgcggtg acgagttcca cgacgatgga
    16261 ctggaccacg agcagctcaa ggaaatcgcc cacgccatgg ccgacaccgg gctggtcgac
    16321 tacatcagcg tggtcggctc gggggcggac acacacaaca ccctggccaa ctgcatgccg
    16381 cccatggccc tgccgccaga accgttcgta cacctagcgg ccggcatcaa atcggtggtg
    16441 cgcattcccg tcatgcacgc gcaaagcatc cgcgacgcga atcaggcgga gcgcatactg
    16501 gccgccggcc tggtcgacct ggtgggcatg acacgcgccc agatcgccga cccgcacatg
    16561 gtcatcaaga tccgcgacgg ccgcgaagac gagatcaagc agtgcgtggg cgccaactac
    16621 tgcatagacc gccagtac
//
LOCUS       DASTNK010000169        17517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1458461, whole genome shotgun sequence.
ACCESSION   DASTNK010000169 DASTNK010000000
VERSION     DASTNK010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 17517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17517
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458461"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VFR20_12375"
     CDS             complement(<1..1023)
                     /locus_tag="VFR20_12375"
                     /inference="COORDINATES: protein motif:HMM:NF004834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0231360.1"
                     /translation="MRHDTDSNHPNRCCIAGGGPAGLMLGLLLARAGVSVTVLEKHGD
                     FLRDFRGDTVHPSTLQILKELNLLEEFNRLPHRRVDHLSGMFAGREQQIVDFRGLKPF
                     DYLALVPQWDFLDMLAAAGKRYPTFDLRMRHEVTNLMQENGRTTGVRVKTPTGESELR
                     ADLVVACDGRRSAVRDAAGLRAREYGAPMDVLWLRLPRRNDDPPDTFGIADRGHVMIL
                     LNRDDYWQTAFVVPKGRDAALRAQPISVLRDAIARLAPFLADRTDSIESWGKVSTLEV
                     RVDRLERWHKPGLLLIGDAAHAMSPVGGVGINLAIQDAVAAANALALPLRGNGAIDES
                     LLAAIQQ"
     gene            complement(1036..1461)
                     /locus_tag="VFR20_12380"
     CDS             complement(1036..1461)
                     /locus_tag="VFR20_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEU0231361.1"
                     /translation="MYVFVYGTLRAGEANDIGDVARRHGLPEPRRVETTTVPGTLHDF
                     GNYPGWVPNDEAATLVVGDVYDVAPNLIPILDDIEEVYPGRPGLFTRTTLSLPCAGAM
                     LLCMAYPVDPSVAATRPVIEGGDWVAYRRSGPVRPNPIP"
     gene            1725..2438
                     /locus_tag="VFR20_12385"
     CDS             1725..2438
                     /locus_tag="VFR20_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004553610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEU0231362.1"
                     /translation="MREIRALLFDVFGTVVDWRNGVANHARPFLERHGSRIDPGMFAD
                     AWRRRYQPAMEAVRIGRRPFVPLDTLHRENLEAILPDAGIDPGAVDPAELDALNLAWH
                     RLDPWPDVLSGLGRLKKRYIIAPLSNGNIVLMLDIAKRAGLPWDAILGAEVVQAYKPT
                     PQAYDRTVALLALRPGQACMVAAHNSDLAAARRSGLRTAFVARPSEHGPGQNTDLVPE
                     QDWDVVAMDFNALAEGLGA"
     gene            complement(2522..3592)
                     /locus_tag="VFR20_12390"
     CDS             complement(2522..3592)
                     /locus_tag="VFR20_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0231363.1"
                     /translation="MSANNEHQIDGEGRPAGRARVTDIAASAGVSTATVDRVLNHRPG
                     VRSATAQRVLKAATQLDYLPEQALREALAPAPMRIVFLLPKGTNRYLTMLGDTVHYSL
                     DNWSPFNVRCRVEYIEGFNPQLLAAALTRQSERADGVAFMAIEHPLVRDAVNQLVARG
                     VHVVTLITDISGSGRAAYLGLDNRAAGRTAAYLIGRFIGPRPAKVAMIAGSLSYRAHE
                     EREMGFLYLFREQFPGIEVVGLREGQDNADRNYRLTRQLLEQAPELAGIYNIGGASDG
                     VARALKEAGLEHKVVFIGHGLTPDTRALLIDGTMDAIITQNPQSTMSSSVRIFSNLRD
                     HRPATSGVESVQSQIIFRENLP"
     gene            3716..4735
                     /locus_tag="VFR20_12395"
     CDS             3716..4735
                     /locus_tag="VFR20_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012429008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEU0231364.1"
                     /translation="MKNLTRRSMLKALSTLPLAGAAGTFPLVARAAEYSYKYGNNLPL
                     SHPLNVRAHEAADEIRAKTKGKVDIKIFPNNQLGGDTDMLSQVRSDGIQFFTPSSLVI
                     ATLVPVAAINAVGFAFSDYAQVWKAMDGNVGAYVRGAIDKVGLYAFEKMWDNGFRQVT
                     SSKAPIKSAADMADLKIRVPVSPLSIAMFKALGAAPTSLQFSEVYSALQTKIVDAQEN
                     PLPIIQVAKLYEVQKYCSLTNHIWDGFWFIANGRAWKRLPKDLQTVVADAINGAGVRQ
                     REDIKKLNESVQADLQAKGLQFNQVASDSFRAKLREAGFYKTWKGRFGAEAWGVLEQS
                     VGNLA"
     gene            4744..6621
                     /locus_tag="VFR20_12400"
     CDS             4744..6621
                     /locus_tag="VFR20_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEU0231365.1"
                     /translation="MAGLGTTGSAAISSRNVGLKRVAAGLENGLGRTIELLCAALLVV
                     EIVVLFSGVVARYVLHVPLVWSDELASMLFLWLSMLGAVVALRRGEHMRMTALVDRVG
                     PRWQAFLDAFAQTASLAFLLLVIVSACDYARDESFIITPALQVNDLWRAAALPVGLGL
                     MALVSLLRLIRVRDARTVLTAIACTIGLALGFWLAQPVFAGLGNINLVIFFVVLVAVN
                     VFSGVPIAFSFALATFGYLALSTDTPMVVMMGRLNEGMSHLILLAVPLFIFLGALIEM
                     TGMARAMIQFLAGLLGHVRGGLSYVLIGAMYLVSGISGSKIADMAAITPVLFPEMQKR
                     GAKPGELIALLAATGAQTETIPPSIVLITIGSVTGVSIAALFDGGILPAVVLGLSLCF
                     VVWRRCRHDDLSQVKRVERKQILRLFVVALPAVCLPFVIRAAVVEGVATATEVSTIGV
                     VYTIAIGLFVYRHFEWKRLVPMLVETSSLTGAIMLIIGCATAMAWALTQSGFSSQLAH
                     FMAGVPGGSYGFMAISVLTFIILGSVLEGIPAIVLFGPLLFPVARELGINEVHYSMVV
                     IFSMGIGLFSPPFGVGYYGACAISRVDPSDGMRHIWGYIAALLVGLAVVTAVPWLSTG
                     L"
     gene            6681..7220
                     /locus_tag="VFR20_12405"
     CDS             6681..7220
                     /locus_tag="VFR20_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0231366.1"
                     /translation="MSRHFGEIRQLGYVVRDVEQAMKYWSETLGVGPWYYNPQVPIVN
                     YQYEGRRYEPHNSVALANSGFIQVELIQVRNDVPSMYRDFLLQGFTGLQHVAYWTEDY
                     DADLRRLEGMGFRPKMGGEVGQRGRFVYFDTGLYPGTVIELSEVAGPKGRLFDLIRDA
                     AQGWDGKDPVRDFPDLDTL"
     gene            7250..8563
                     /locus_tag="VFR20_12410"
     CDS             7250..8563
                     /locus_tag="VFR20_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase large subunit
                     family protein"
                     /protein_id="HEU0231367.1"
                     /translation="MGTAVVCAAPGEKTTEGRLEATYVIETPYDPSRVAGIMAGEQSC
                     GTFARVRDETDELRARAGATVESVHELDPADTPALRSAFLDKRGVSGPWRRAEVRLSF
                     PLANIGVNLPTLAATVAGNLYDLGEVTGLRLESLRLPSAYRMRFDLPTQGIVGTRARM
                     GAPAGCLIGTIIKPNVGLTPQQTAALVKELCEAGVDFIKDDEVCANPAHAPLAERVPA
                     VMAVVRDHAQRTGKKVMVAFNISDDVDAMRRHAEMVHREGGDCIMASLNWCGFSAIQA
                     LRRSTPLALHGHRNGFGALSRHPLLGMSYQAWQSLWRLTGVDHMHVHGLGGKFAQPDQ
                     EVVDAARDCGQPMSDSIDDRVLPAFSSGQWAGTVPDTWDAVGSEDLLFLAGGGILAHP
                     DGPAAGVASIRQAWQAQREGMSLEVFSRDRPALRVALRRFGESAS"
     gene            8560..9909
                     /locus_tag="VFR20_12415"
     CDS             8560..9909
                     /locus_tag="VFR20_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEU0231368.1"
                     /translation="MKLAWYGDDFTGASDTLAVATQAGLRSMLFLNVPTPARLAAAGP
                     LDALGIAGSTRTMAPQRMREELFPVRDFFQTLDARVFHYKCCSTFDSAAGTGSIGAAM
                     HALRPVAASNVTYVVGGQPDIGRYCCFSNLFAAAGSGDEVFRLDRHPTMSAHPVTPMH
                     ESDLRRVLEGQGVAPIVSVHYPEYRLAESELDARVDGLSGVIDAGQAGALLFDVAEPA
                     HLAAVGRQIWRRASEATLLTAGSSTVLQALAAHWDSVDAKQDARRGQPHRRGGKPETA
                     SRHDGPPVLALAGSMSPVTARQVARAMSYQPLALDARQLISDPAYPRAQANRAVAALE
                     AGRHVLAYVRQSGTGPDHQVSSRDLARCTACLLADILSASARFGGLRRIGVAGGDTSS
                     LAVKALDIWALSFVRAVSPGVALCRAHSDNPTLDGMEVMLKGGQMGPVDIFERLLVD"
     gene            complement(10209..10439)
                     /locus_tag="VFR20_12420"
     CDS             complement(10209..10439)
                     /locus_tag="VFR20_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231369.1"
                     /translation="MPPITDLWPFLKWVACFPGNYAMHLISDTWPGIGDFLGITHHAP
                     FGEPAWGFSFFVWLVALFIFTELVAKPRRRKA"
     gene            10573..10797
                     /locus_tag="VFR20_12425"
     CDS             10573..10797
                     /locus_tag="VFR20_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231370.1"
                     /translation="MKSLMLYARQVGQRRTSAFYLLPKPDSQAGERGHHVFGNRPGMA
                     DRPRSLREMFADTEYDNDYPADDVGLDDTP"
     gene            complement(10838..11143)
                     /locus_tag="VFR20_12430"
     CDS             complement(10838..11143)
                     /locus_tag="VFR20_12430"
                     /inference="COORDINATES: protein motif:HMM:NF021390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEU0231371.1"
                     /translation="MTLKTLPLGLFVAVALSGCGTAASKIPAAFFTCADGSHFSASFP
                     EGEALITLPDQKTLTLPQQRAGSGYWYSSGRYELRGKGKDATWTVGRRAPTSCRSQP"
     gene            11462..12340
                     /locus_tag="VFR20_12435"
     CDS             11462..12340
                     /locus_tag="VFR20_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU0231372.1"
                     /translation="MTPALSTITDRVVATSATMSRTQRRMADYVLSHQFKVATMTIDE
                     FAKAAGVSIATANRFARHLGLQGYPQFRSELARGFQAALAPIEKLRSELAQPTSVAEV
                     FSASLQEDIHNFQATQATLATGQCERVVDLILSADRIFTLGFGGSGYLASLLQRGLCT
                     SCDYVESLAGAGGASHAARRLSRATPRDLVIAISFPRYVKDTVLLARAARKAGSSLIV
                     LTDSPTSPLAPDATLTLYVSSERQFSATSETAVLGLIEALCAATAYKAKNSLESATGL
                     AESVMPWLTFGEDSHG"
     gene            12333..13301
                     /locus_tag="VFR20_12440"
     CDS             12333..13301
                     /locus_tag="VFR20_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEU0231373.1"
                     /translation="MVEPVLVIHGGAGALTRSVMSNDAEREYRAALTGILEAASAALN
                     QGGSALDCVTQAVCLLEDCALFNAGKGAVFTSAGTHELDAAIMDGATLESGAIANVEH
                     VKHPILAARAVMEHSSHVFFVSRGAEDFALGQGLEMVENDYFSTPHRREQWLRVVNEM
                     PGQAVLDHDAARAEAAAHAPLDPDRKFGTVGAVAVDAQGNLAAATSTGGVTNKQVGRV
                     GDSPLIGAGCYAANGTAAVSATGTGEMFIRAVAAHTVAAMMEFGGATLEQATDSVVHE
                     RLAAIKGQGGLIAVDGQGNVAMPFNTEGMYRGYARRGEKPFVAIYR"
     gene            13308..15254
                     /locus_tag="VFR20_12445"
     CDS             13308..15254
                     /locus_tag="VFR20_12445"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEU0231374.1"
                     /translation="MVEDTLSHAGTGAKDSNAGFPENRVLEVSDLSVEFTSQQRTVRA
                     VNHVSLHVDFGETLAIVGESGSGKSVTSLSLMRLVEHGGGRIAGGRILVRRRDDSILD
                     VTATSRDTLRTMRGADMAMIFQEPMTSLNPVFSVGEQISESIRLHQGKDSAAASAEAL
                     RMLERVRIPEARAMLDRYPHQLSGGMRQRVMIAMALACKPALLIADEPTTALDVTIQA
                     QILQLIRELQQEMRMGVIFITHDMGVVAEVAHRVLVMYRGDKVEEGPAERVFAAPAHA
                     YTKALLSAVPRLGAMNGTDLPAPFPLLDLAAVQGAVPGAAKRQMETVDVPLPEAPSAE
                     ATRTPILSVKDLVTRFDVRGGLFGRVQRRVHAVEKVSFDLYPGETLALVGESGCGKST
                     TGRSLLRLVQTQQGSVTFDGHRIDTLQGRELQALRRNIQFIFQDPFASLDPRLTVGFS
                     IMEPLLIHKVATPRQAQETVERLMDRVGLPPEMAHRYPHEFSGGQRQRLCIARALALN
                     PKVIIADESVSALDVSIQAQIVNLLIGLQEELGVSYLFISHDMAVVERISHRVAVMYM
                     GQIVEIGPRRAIFENPQHAYTKKLMSAVPIADPSRRHMAHTLLSGEIPSPLRRLGDEP
                     VVEPLVECGRDHFVARHAVGGGLA"
     gene            15392..16942
                     /gene="gsiB"
                     /locus_tag="VFR20_12450"
     CDS             15392..16942
                     /gene="gsiB"
                     /locus_tag="VFR20_12450"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HEU0231375.1"
                     /translation="MLERFKPAGVVIAAALAFGAFHVPVAMAAKNVTVAVPYAIDSLD
                     PYNTNSTLSMAVGKAYYEGLFAFDKNLKIVDLLATGYSVTPDGLIYTIKLRHGVKFQD
                     GTDFNAAAVKANLDRVTNKANHLARYNQFNRIKSVEVVDPYTVKITLTEPFSPFINSL
                     AHPAAMMISPTALKKWGKDIAFHPVGTGPFEFVEWKPSEYLKVKKWDGYWDKGLPKID
                     SLTFRTVTDNNTRAAVVQTGEAQFVYPVPYEQANILKKNPKLEVVVSPSISVKYVSFN
                     MLQKPFDNLKVREAINYAINKKALIKVAYGGYGEPMNGVVPTSVDYGVKMKPWPYDPA
                     KARELLKEAGYPHGFTTTLWSGYNDGTSVKAVQFLQQQLAQVGIKASLTVLESGQRVQ
                     QVQQVQNPQDAKVRMFYAGWSSSTGEADWALRPLFDSKDWPPVLNNTAYYKNPKVDED
                     IAQALLTTDRAKKQALYTDAQERIWKDAPWAFLAIPSNISAQSKKLTGMYVMPDGSFS
                     FRDIDLKQ"
     gene            16990..>17517
                     /locus_tag="VFR20_12455"
     CDS             16990..>17517
                     /locus_tag="VFR20_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012280.1"
                     /note="with GsiABD is involved in the transport of
                     glutathione into the cell; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HEU0231376.1"
                     /translation="MFIYIIKRLVGMVITLLLVAMVVFLFVRMLPGDPARLAAGPEAD
                     QQTVNLVRQELGLDKPLPVQFVRYLDGLMHGKMGTSLRTRQPVETVIAERFMPTFWLT
                     LLSMLWSVTFGMVIGIVSSVWRNRWPDRLCMTLAVSGISFPAFALGMMLIQIFSVQLG
                     WLPTVGASTPAHYILP"
BASE COUNT         3087 a         5191 c         5746 g         3493 t
ORIGIN      
        1 ttgctggatg gcggcgagca gagattcgtc gatggcaccg ttgccgcgca acggcaaggc
       61 cagcgcgttg gctgcggcga ccgcatcctg gatcgccaga ttgatgccga cgccgccgac
      121 cggtgacatc gcgtgcgcgg catcgcctat cagcagcagg cccggtttgt gccagcgctc
      181 gaggcggtcg acccggacct cgagcgtact gaccttgccc caggattcga tactgtcggt
      241 gcgatcggcc agaaagggcg ccaatctcgc gatcgcgtcg cgcaggacgc tgatcggttg
      301 agcgcgcagc gccgcgtcgc ggcctttggg caccacgaac gcggtttgcc agtagtcgtc
      361 gcggttgagg aggatcatca cgtggccgcg atccgcgatg ccgaatgtgt cgggcgggtc
      421 gtcgttgcgg cggggtaggc gcaaccacag cacatccatg ggcgcgccat attcccgcgc
      481 ccgcagcccg gcggcatcgc gcacggcgga gcgtcggcca tcgcaggcaa ccaccaagtc
      541 ggcgcgaagc tcagactcgc ctgttggcgt cttgacacgc acaccggttg tgcggccgtt
      601 ctcttgcatc agattggtga cttcgtggcg catgcgcaaa tcgaaggtgg ggtagcgctt
      661 gcccgccgcc gccagcatgt ccaggaagtc ccactgtggc acgagagcca ggtagtcgaa
      721 cggcttcaat ccgcggaaat ccacgatttg ctgttcccgc ccggcgaaca tgccgctcag
      781 atggtccacg cgtcggtggg gtagccggtt gaattcctcg agaaggttca gttccttcag
      841 gatctgcaga gtcgagggat gcacggtgtc cccgcgaaaa tcgcgcagga aatcaccatg
      901 cttttccagc accgtcaccg acacacccgc acgcgccagc aacaagccca gcatcagccc
      961 cgcggggcca ccacctgcga tgcagcaccg gttggggtga ttggagtccg tgtcatgccg
     1021 catggcgccc ccatttcaag gtatcgggtt tggtcggact ggcccggacc gccggtaggc
     1081 cacccagtcg ccaccttcga tcaccggccg cgtcgcggcc acggatgggt caacgggata
     1141 agccatgcac agaagcatgg ctccggcgca aggcagagac aatgtggtcc gcgtgaacaa
     1201 gccgggcctg cccggataga cctcttctat gtcgtccagg atggggatga gattcggcgc
     1261 gacgtcgtac acatcgccca cgaccagggt ggccgcctcg tcattgggta cccagcctgg
     1321 gtagttgccg aaatcgtgca atgtgccggg cacggtggtg gtctcgaccc gtcgcggttc
     1381 aggtaggcca tgtcgtctcg cgacatcccc gatgtcattg gcctcgccgg cgcgcagcgt
     1441 gccatagacg aagacataca tcgtgtcgtc ttgaccgttg gcgtggccgc gtatgtcgtg
     1501 ggtggcccat ttatctttga cagactgttt gatcttggct gacatgaagg cggtcactca
     1561 cgtggtggac ggtggcacgg atggcgattg gcgcatcagc atcttacccc ccgttcatcc
     1621 gttttcacac actacaattc aatttgagat tatcgccacg ttggccggtg cggggctcta
     1681 ttcagcgtga gcaggccttg gccaaaacca tgaggggatg ggtgatgcgt gagatccgcg
     1741 ctttgttgtt cgatgtgttt ggcacggtgg tcgactggcg caacggcgtt gccaaccatg
     1801 cccggccgtt tctcgagcgc catggttcgc gcattgatcc gggcatgttc gcagatgcct
     1861 ggcgccggcg gtatcagccc gccatggaag cggtgcggat cgggcgccgg ccattcgttc
     1921 cgctcgacac gctgcatcgg gaaaacctgg aggcaatcct gccggatgcg gggatcgacc
     1981 cgggtgcggt cgatccggcc gagctcgacg cgttgaacct ggcctggcat cggcttgatc
     2041 cctggccgga cgtgctgtcg gggctggggc gcctgaagaa gcgctacatc atcgcgccgc
     2101 tctccaacgg caacatcgtg ctcatgctgg atatcgccaa gcgcgcgggc ctcccgtggg
     2161 acgccatcct gggggcggaa gtggtccagg cctacaagcc cacaccgcaa gcctatgatc
     2221 gcacggtggc gctgctggcc ttgcgacccg ggcaggcctg tatggtggcg gcgcacaact
     2281 ccgatctggc ggcggcgcgg cgcagcggtc tgcgcaccgc gttcgtggcc cgcccaagcg
     2341 agcacggccc ggggcaaaac accgacttgg tccccgagca ggactgggat gtcgttgcca
     2401 tggacttcaa tgcgctggcc gaggggttgg gcgcgtaggg ccaagctcgg cggtagaccc
     2461 cggcaatagc gccttccgca ttgtgacccg gagtaggggg tcttggatgt tcagccctgc
     2521 ttcagggcag gttttcgcga aatatgatct ggctttgcac cgactcgacg ccactggtgg
     2581 cggggcgatg atcgcgtaga ttggaaaaga ttctgacgct gctcgacatt gttgactggg
     2641 ggttctgcgt gatgatggcg tccatcgtgc cgtcgatcag cagtgcgcgt gtgtcgggcg
     2701 tcagcccatg gccgatgaaa accaccttgt gttccagccc tgcttccttc aatgcgcggg
     2761 ccaccccgtc ggacgccccg ccaatattgt agatacccgc cagttccgga gcctgctcca
     2821 gcagctggcg tgtgagccgg taattgcgat cggcgttgtc ctggccttcg cgcagaccca
     2881 ccacttcaat gcctggaaac tgttcgcgaa aaaggtatag aaagcccatt tcgcgttctt
     2941 cgtgtgcacg gtagctgagg ctgccggcga tcatcgccac cttggcgggg cgcgggccga
     3001 tgaatcgacc gatcaggtag gccgcggtac gcccggcggc gcgattgtcc aggcccagat
     3061 aagcggcgcg gccggagccg gaaatgtcgg tgatcaatgt gaccacatga acgcctctgg
     3121 ccacgagctg gttcacggca tcgcgcacca gggggtgttc gatggccatg aaagccacgc
     3181 cgtcggcacg ctcggactga cgagtcaggg cggcggcgag caactggggg ttgaagcctt
     3241 ctatgtactc cacgcgacag cgcacgttga atggcgacca gttgtccagg gagtagtgca
     3301 ccgtgtcgcc cagcatggtc aggtaacggt tggtgccttt cgggagcagg aagacgatgc
     3361 gcatgggcgc gggcgccaat gcttcgcgca atgcttgctc gggcaggtaa tccagttgcg
     3421 tggcagcctt cagcacgcgt tgcgccgtgg cggagcgcac gccgggccgg tgattcagca
     3481 cccgatcgac cgtggcggtg gagaccccgg ccgaggcggc gatatcagtg acccgcgccc
     3541 gtcccgcagg acgaccctcg ccgtcgattt gatgttcatt atttgctgac atacatcaaa
     3601 aacaatcatt tgtggtgttt gaccttatta tgattgcacg attatctttg caatacaaga
     3661 aagacgttat cgcagctatc aatcatatta catcaaatca gatcaggaga cattcatgaa
     3721 gaatttgact cgtcgcagta tgctgaaggc gctgtccacg ctacctctgg ccggcgcggc
     3781 cggtaccttc ccgctcgtgg ccagggccgc cgagtattcc tacaaatacg gcaacaatct
     3841 gccgctgtcg catccattga acgtgcgcgc ccatgaagcg gccgacgaga tccgcgccaa
     3901 gacgaaagga aaggtggaca tcaagatttt ccccaataac cagcttggcg gtgacaccga
     3961 catgctgtcg caggtgcgtt ccgacgggat ccagttcttc acgccgtcgt ccctggtgat
     4021 cgccacgctg gtgccggtcg cggcgatcaa tgccgtgggc tttgccttct ccgactatgc
     4081 ccaggtctgg aaggccatgg acggcaacgt gggggcttat gtccgtggcg cgatcgacaa
     4141 ggtggggttg tatgcgttcg agaagatgtg ggacaacggt ttccgtcagg tgaccagcag
     4201 caaggcgccc atcaagtccg ccgccgacat ggccgatctg aagatccgcg tcccggtgag
     4261 ccctttgtcg atcgccatgt tcaaggcgct gggcgcggca cccaccagcc tgcaattcag
     4321 cgaggtctac tccgcgctgc aaacaaagat cgtcgacgcc caagagaacc cgctacccat
     4381 catccaggtg gccaaactgt acgaagtgca gaaatactgc tcgctgacga atcacatttg
     4441 ggatgggttc tggttcatcg ccaatgggcg ggcgtggaag cgcttgccga aggatctgca
     4501 aacagtcgtc gctgacgcca tcaatggggc tggcgtccgc cagcgcgagg acatcaagaa
     4561 gctgaatgag tcggtgcagg ccgatttgca agccaaggga ttgcaattca atcaagtcgc
     4621 gtccgacagt ttccgcgcga aactgcgcga ggctggcttc tacaagacct ggaagggacg
     4681 ctttggcgcc gaggcctggg gcgtgctgga gcagtccgtc ggcaacctgg catgagcacg
     4741 gccatggctg gcttgggcac aaccggcagc gcggcaataa gcagccggaa tgtcggcttg
     4801 aaacgggtgg ccgcgggtct ggaaaatggg ctggggcgca ccatcgaact gctttgcgcg
     4861 gcgttgctgg tggtggagat cgtcgtgctg ttcagcggcg tggtggcacg gtatgtgttg
     4921 catgtgccgc tggtgtggtc cgacgagctc gcgtccatgt tgttcctttg gttgtcgatg
     4981 ctgggggcgg tggtcgcgtt gcggcgcggc gagcacatgc gcatgacggc tctggttgat
     5041 cgtgtggggc cgcgctggca agcctttctg gatgcgttcg cacagacggc gtccctggcg
     5101 tttcttttgc tggtcatcgt gtccgcctgt gactatgcgc gggatgagtc gttcatcatc
     5161 acccccgcgc ttcaggtcaa cgatctgtgg cgggcggcgg cattgcccgt cgggctgggg
     5221 ttgatggcgc tggtgtcatt gctgcgcctg attcgtgtgc gtgacgcgcg cacggtgttg
     5281 acggccattg cctgcacgat cggcctggca ctggggttct ggttggctca gcccgtgttc
     5341 gctggcttgg gcaacatcaa tctggtgatc ttcttcgtcg tcctggtggc ggtcaacgtg
     5401 ttttccgggg tgcccatcgc gttctccttt gcgctggcca cgttcggcta cctggccttg
     5461 tcgacggata cgcctatggt ggtgatgatg gggcgcttga acgaaggcat gtcacatctg
     5521 atcctgctgg cggtgccgct cttcattttc ctgggcgcgt tgatagaaat gaccggcatg
     5581 gcccgggcga tgatccagtt cctggcagga ttgctggggc atgttcgggg cggattgtcg
     5641 tatgtattga ttggcgcgat gtatctggtg tctggtatct cgggttcgaa gatcgccgac
     5701 atggcggcga tcaccccggt gctgtttccg gaaatgcaga agcgcggcgc caagcctggc
     5761 gaactgatcg ccctgctcgc cgcgacgggt gcgcagaccg agaccattcc gccctccatc
     5821 gtgttgatca ccatcggctc tgtcaccggg gtgtccatcg ccgccctgtt cgatgggggc
     5881 atcctgcccg ccgtcgttct gggcctgtcg ttgtgcttcg tggtctggcg gcgttgccgc
     5941 cacgatgact tgagccaggt caagcgcgtg gagcgcaaac agatcctgcg cttgttcgtc
     6001 gtcgcgctgc cggctgtttg cctgccgttc gtcattcgtg ccgcggtggt ggagggtgtg
     6061 gcgaccgcca cggaagtctc gaccattggc gtcgtgtaca cgatcgccat cgggctgttc
     6121 gtgtatcgcc atttcgaatg gaagcggctg gttccgatgc tggtggagac gtcctcgctg
     6181 acaggcgcca tcatgctcat catcggctgc gccaccgcga tggcctgggc gctgacgcag
     6241 tcagggtttt cgagccagtt ggcgcacttc atggcgggtg tgccgggtgg gtcgtacggc
     6301 ttcatggcga tctcggtcct cacgttcatc atcctgggca gtgtgctgga aggcatcccg
     6361 gccatcgtcc tgttcggtcc attgctgttt ccagtggcac gggagctggg catcaacgaa
     6421 gtccactatt ccatggtcgt gatcttttcc atgggcatcg gcctgttctc gccgccgttc
     6481 ggcgtgggct attacggcgc gtgcgcgatc agccgggtcg atccgagcga cgggatgcgc
     6541 catatctggg gatacatcgc ggcgttgctg gtgggactgg ccgtggtgac cgctgtgccc
     6601 tggttgtcca ctggtttgta aatcagaaat tgcgtgtcgc cgtcataccc gtcgtgcgac
     6661 acttagagga gaagaagcaa atgagtcgtc atttcggaga aatccgccaa ctgggctatg
     6721 tggtgcgtga cgtcgagcag gccatgaaat actggagcga gacgctgggt gtggggccct
     6781 ggtactacaa cccgcaggtt cccatcgtta actaccaata cgaggggcgg cgctacgaac
     6841 cgcataattc ggtcgcgttg gccaattccg gcttcatcca ggtggaattg atccaggtac
     6901 gcaacgacgt gccctccatg tatcgcgatt ttcttctgca ggggttcaca ggattgcagc
     6961 atgtcgccta ctggaccgaa gactatgacg ctgatctacg gcgtttggag ggcatgggct
     7021 tccgtccgaa aatgggtggt gaagtggggc agcgcggtcg cttcgtctat ttcgataccg
     7081 gcctgtatcc cgggacggtg atcgaattat ccgaagtggc cggccccaag ggcaggctgt
     7141 tcgatctcat ccgcgacgcg gcgcaaggct gggacggcaa ggatcctgtg cgtgacttcc
     7201 ccgacctgga tacgctatga gccgtatcgt cgacgcgggg atccccagga tgggcacggc
     7261 ggtggtgtgc gcggcgccag gagaaaagac cactgagggt cgtctggaag ccacctacgt
     7321 gatcgagacg ccgtacgatc caagccgtgt tgccggcatc atggcgggcg agcagtcgtg
     7381 cggcacgttc gcgcgggtcc gggacgagac cgatgagttg cgcgcccggg ccggcgccac
     7441 ggttgaatcg gtccatgagt tggatccggc cgatacgcct gctctgcgga gcgcatttct
     7501 ggacaagcgc ggggtgtccg ggccgtggcg ccgtgccgag gtgagattgt cgtttccgct
     7561 ggccaacatc ggcgtgaacc tgcccacgct cgcggccacg gttgcgggca acctatacga
     7621 cctgggggag gtgacgggct tgcgtctgga gtcgctgcgc ctgccgtcgg cttatcgcat
     7681 gcgattcgac ctgccgacgc aaggtattgt cggtacgcgc gcccgtatgg gcgctcccgc
     7741 gggttgcctg atcggcacca tcatcaaacc caatgtcggt ctgacacccc aacagaccgc
     7801 ggcactggtc aaggagttgt gcgaagcggg agtggacttc atcaaggacg acgaggtgtg
     7861 cgccaatccc gctcacgcgc cactggccga gcgtgtgcca gccgtgatgg cggtggtgcg
     7921 cgaccatgcg cagcgcactg ggaaaaaagt gatggtggcg ttcaacatca gcgatgatgt
     7981 cgacgcgatg cgtcgtcacg cggagatggt gcatcgcgaa ggtggggatt gcatcatggc
     8041 cagcctgaac tggtgcgggt tctctgcgat ccaggccttg cgccgcagca cgcccttggc
     8101 cctgcacggg cacagaaacg gctttggcgc attgtcgcgc catcccctgc tgggcatgtc
     8161 gtatcaggcg tggcagagtc tctggcgcct gacgggtgtg gaccacatgc acgtgcacgg
     8221 gctgggcggg aagttcgcgc agccggatca ggaagtcgtc gatgccgcgc gtgattgcgg
     8281 tcagccgatg tccgattcca tcgatgatcg ggtcttgccg gctttctcgt cggggcaatg
     8341 ggcgggtacg gttcccgaca cctgggatgc ggtgggaagc gaagatctgc tgttcctggc
     8401 gggtggcggc attctggcgc acccggacgg cccggcggcg ggcgtggcca gcatacgcca
     8461 ggcctggcag gcccagcgag agggtatgtc gctggaggtt ttcagtcgag atagaccggc
     8521 actgcgtgtg gcgttgcggc gttttggtga atccgcctca tgaagctggc ttggtacggg
     8581 gatgacttca ccggcgcgag cgacacactc gctgtggcga cgcaggccgg attgcgaagc
     8641 atgctgttct tgaatgtgcc gacaccggct cggctcgcgg ccgccggccc gttggatgcg
     8701 ctgggcatcg ccggatcgac gcgcacgatg gcgccgcagc gcatgcggga ggaattattc
     8761 cccgtgcgtg atttctttca aacgctcgat gcacgagtgt tccactacaa atgctgttcc
     8821 acgttcgata gcgcggccgg gactggcagc atcggtgcgg cgatgcatgc gttgaggcct
     8881 gtcgcggcgt cgaacgtgac ctatgtcgtt ggtggccagc ccgatatcgg gcgctactgc
     8941 tgcttcagca atctgtttgc ggcggcgggg tccggggatg aggtgttccg tcttgatcgc
     9001 catccaacaa tgagcgcgca tcccgtcacg cccatgcatg aatccgatct gcggcgcgtt
     9061 ctggaggggc agggcgtggc gccgatcgtc agcgtccact accccgagta caggcttgcc
     9121 gagagcgaat tggacgccag ggtggatggg ctttccgggg tgatcgacgc cgggcaggcg
     9181 ggtgccttgc tgttcgatgt cgcagagcca gcgcatttgg ccgcggtggg tcggcagata
     9241 tggcggcgcg ccagcgaagc cacgctgttg actgctggtt cgagtaccgt gttgcaggcg
     9301 ttggcggcgc attgggattc cgttgacgcc aagcaagatg caaggcgggg acagccgcat
     9361 cggaggggag gcaagcccga gacagcgtcg cgccacgatg gcccgcccgt tctcgcgctg
     9421 gcgggaagca tgtcacccgt gacggcccgc caggttgccc gggcgatgtc gtatcagccc
     9481 ttggccctgg acgcgagaca gctgatctcg gaccccgcct atccacgggc gcaggccaac
     9541 cgggctgtcg ccgcattgga agcaggccgg catgtgcttg cttatgtgcg gcaatccggc
     9601 acgggccccg atcaccaggt gtcgtcgcgc gatttggcca ggtgcacggc gtgtctgttg
     9661 gccgatatcc tgagcgcctc ggcgcgcttc ggcggcctgc ggcgcattgg tgtggcggga
     9721 ggcgacacat cgagcctggc ggtcaaggcg ctcgacatct gggccttatc gttcgttcgc
     9781 gcggtgagcc ccggcgtggc cttgtgccgt gcccacagcg acaacccgac gctcgatggc
     9841 atggaagtga tgctcaaggg ggggcaaatg gggcccgtgg atatcttcga gcgtctgttg
     9901 gtggattgac gcgcgatcga aagttgtttc agcgtacccc gcgttcccgg gcgtggttcg
     9961 tggattgacg cggcatttgt ttagccttgg tcaaagtggt tacgttctgc aagatgcctg
    10021 tgtcgcgcac tgcctagaat ccatgcatcg acatgtattc caggtggctt tgatgaagat
    10081 tcttatcgcg atcgtgctgg cttcgacttt ggcgggctgt atcatcgttc ccgcgcgggg
    10141 ctatcaccat cattggcacc acgattgctg ctattaccgt taagtatcct taaagcctgt
    10201 caggcctttc aagctttgcg ccttctgggt ttggcgacaa gttctgtgaa aatgaacagc
    10261 gccacgagcc agacgaagaa ggaaaacccc caggccggct cgccgaatgg cgcgtggtgg
    10321 gtaatgccca ggaaatcacc gatgccgggc caggtgtcgg aaatgaggtg catggcatag
    10381 ttgcccggaa agcaggcaac ccacttgagg aatggccaga gatcggtgat cgggggcatg
    10441 gggcttataa tcgggaccga cagaaaaacg ccctattatg gagtggaaac accccgggtt
    10501 ccatagggag aatgcctgcg gttcgcgggt gtggcgccac cgccagggtg gcaagagaaa
    10561 gaagagggta gtgtgaaatc attgatgctg tatgcgcgtc aggttggtca gcgacgcacg
    10621 tcggcgttct atttgcttcc caagcctgat tcccaggcgg gggagcgtgg tcatcatgtg
    10681 ttcggtaatc ggcccggcat ggcggaccgt ccccgctccc tgcgtgaaat gttcgccgat
    10741 accgagtatg acaacgatta ccccgccgat gacgtcggac tggacgatac gccctgattc
    10801 aggcatcacc gtgcacccgc ttggtcagaa aaatgcctca cggttgtgat cggcagctgg
    10861 tcggggcacg ccggcccacc gtccaggtgg catccttgcc tttgccccgc agttcgtatc
    10921 gacccgacga ataccaatag ccagagccag cccgttgttg cggcagggtg agggttttct
    10981 gatcgggcag cgtgatcagg gcttcgcctt cggggaagga cgccgagaag tggctgccgt
    11041 cggcacaggt gaagaacgct gcggggatct tgctcgcggc cgtgccgcag ccagacaggg
    11101 cgaccgcgac aaacagcccc agtggaagtg ttttcaaagt catttcacgc cttttttttc
    11161 atgtcacgat tccccttgct ggttggggtg ccatgcggtg gggtgcgcct gtcaagtgaa
    11221 tttgttggaa cgcttttcat cataccgtcc tggcgatgat cctgtaccgg cgcgcggcat
    11281 ctcaaggaca tcccccgtcg ccgtgagggt gggcatcgcg ggatcatggt ttaccctatt
    11341 atgaaaataa attttcttaa tataatattc acggaaattt aatttcatac acgatgaagc
    11401 tcttgaggca tgcccctgat acggtggggt atggaccaac gagcgcagcg cgtctattca
    11461 catgactcca gccctttcga cgatcaccga ccgcgtcgtg gccacctccg ccacgatgag
    11521 ccgcacccag cggcgcatgg ccgactacgt gctgtcgcat cagttcaagg tcgcgaccat
    11581 gactatcgat gaattcgcga aggccgccgg agtgtcgatc gccacggcca accggttcgc
    11641 gcggcacttg gggctgcagg gctacccaca gtttcgttcc gaactcgcgc gcgggtttca
    11701 ggccgcgttg gcgccaatcg aaaaactgcg ctcggaactg gcacaaccca ccagtgtggc
    11761 ggaggtgttt tcggcgtcac tgcaggaaga cattcacaat ttccaggcga cccaggctac
    11821 gcttgccact gggcagtgcg aacgcgtcgt cgatctcatc ctgtccgccg accgcatctt
    11881 cacgcttggc ttcgggggca gcggctattt ggcatcattg ttgcagcgtg gcttgtgcac
    11941 gtcgtgcgat tatgtcgagt cgctggccgg tgccggcggc gcatcccacg ccgcccggcg
    12001 cctctcccgg gccactcccc gcgatctcgt gatcgccatc tcgtttcccc gctacgtcaa
    12061 agacactgtg ctgttggcgc gcgccgccag aaaggccggc agctcgctca ttgtcctgac
    12121 cgacagtccc acgtcgccgc tggcgccgga cgccacactg acgctgtatg tcagcagcga
    12181 gcgtcagttc agcgccacat cggaaacggc cgtcctgggc ctgatagaag ccttgtgcgc
    12241 cgcgaccgcc tacaaagcca agaactcact ggaatccgcc accgggctgg ccgaatccgt
    12301 gatgccctgg cttacttttg gagaagattc tcatggttga acccgttctg gtgattcacg
    12361 gcggagcggg ggcgttgacc cgttcggtca tgtccaatga cgccgagcgt gaataccgcg
    12421 cggcgctgac cggcattctg gaagcggctt ccgctgcgct taaccaaggt ggcagtgcgc
    12481 tggattgcgt gacccaggcg gtatgcctgt tggaagattg cgcgctgttc aacgcgggca
    12541 agggtgcggt gttcaccagc gccggtaccc atgaactgga tgcggcgatc atggatggcg
    12601 cgacgctgga aagcggcgcg atcgccaacg ttgagcacgt caagcatccc atcctggccg
    12661 cccgggcggt catggagcac agcagtcatg tgttctttgt gagccgcggc gccgaggatt
    12721 tcgccctggg gcaaggcctg gaaatggtcg agaacgatta cttctcgaca ccccatcggc
    12781 gcgagcagtg gctgcgcgtc gtcaatgaaa tgcccgggca ggctgtcctg gatcacgatg
    12841 ccgcgcgtgc cgaggcggct gcccacgcac cgctcgaccc ggatcgcaaa ttcggcacgg
    12901 tcggggcggt ggcggtcgat gcgcagggta atctggcggc cgcgacgtcc actggcggcg
    12961 tcaccaacaa gcaagtggga cgcgtgggcg attcgcccct gatcggtgcg ggatgctatg
    13021 ccgccaatgg gacggccgct gtgtcggcca ccggcacagg cgagatgttc atccgcgcgg
    13081 tggcggccca taccgtcgcc gccatgatgg agttcggcgg cgcgacgctg gagcaggcca
    13141 ccgacagtgt ggtgcatgaa cggctggccg ccatcaaggg gcagggcggg ctgattgccg
    13201 tggacggtca aggcaatgtc gccatgcctt tcaacaccga agggatgtac cgcggctacg
    13261 cgcgccgtgg tgaaaagccc ttcgtcgcca tctacaggta ggccggaatg gtcgaggaca
    13321 ccctttctca cgccgggacc ggcgccaagg attcaaacgc cggcttcccc gagaacagag
    13381 tgctcgaggt ctccgacctg agcgtcgagt tcacctcgca gcaacgcacc gttcgagccg
    13441 tgaaccatgt gtcgctgcac gtcgatttcg gtgaaaccct ggccatcgtg ggggaatccg
    13501 gctctggtaa atcggtcacg tcgctgtcac tgatgcgcct cgtcgaacac gggggtggtc
    13561 gcatcgcggg tggccgcatc ctggtgcgcc gacgtgacga ttccatcctg gacgtgacgg
    13621 cgaccagtcg cgataccttg cgcaccatgc gcggcgccga catggcgatg atcttccagg
    13681 agcccatgac gtcgctgaac cccgtgttct cggtgggcga acagatttcc gaatccatcc
    13741 gcctgcacca gggcaaggac agcgcggcgg catccgccga ggccttgcgc atgctggagc
    13801 gtgtgcgcat cccagaagcg cgcgccatgc tcgaccgata tccgcatcag ttgtcgggcg
    13861 gtatgcgcca gcgcgtcatg atcgccatgg cccttgcctg caagccggcc ctgctgatcg
    13921 ccgacgagcc gaccacggcg ttggatgtga cgatccaggc gcagatcctg cagctcatcc
    13981 gtgagctgca gcaggaaatg cgcatgggtg tgatcttcat cacgcacgac atgggcgtgg
    14041 tggccgaggt ggcgcatcgc gtcctggtga tgtatcgcgg cgacaaggtt gaggaaggcc
    14101 ccgccgagcg cgtgttcgcc gcgccggcgc atgcctacac caaggcgctg ctgtccgcgg
    14161 tgcccaggct gggtgccatg aatggcaccg atctgcctgc tccgtttccg ctgttggacc
    14221 tggcggcggt gcagggggcc gtgccaggcg ccgccaaacg gcaaatggaa acggtggatg
    14281 ttcctctccc cgaggcgcca agcgccgagg cgacgcgcac ccccatcctg tcagtgaagg
    14341 atctggtcac gcgcttcgat gtgcgcggcg gcctcttcgg ccgcgtgcag cggcgcgtcc
    14401 acgcggtgga gaaagtcagc ttcgatttgt atccggggga aacgctggcg ctggttgggg
    14461 aatccggttg tgggaagtcc accaccggcc gctcgctgtt gcgcctggtg cagacgcagc
    14521 agggctcggt gacgttcgat ggccatcgca tcgatacgct ccagggccgg gagctgcagg
    14581 cgctccgacg gaacatccag ttcatcttcc aggatccgtt cgcgtcactg gatccgcgcc
    14641 tgacggttgg cttctccatc atggagccgc tgttgatcca caaggtcgcc acgccgcgcc
    14701 aggcccagga aaccgtcgaa cgcctgatgg accgggttgg cctgccgccc gagatggcgc
    14761 atcgctatcc ccatgaattc tccgggggcc agcgccagcg tctgtgcatc gcgcgcgccc
    14821 tggcgctgaa cccgaaagtc atcatcgccg acgagtcggt gtccgcattg gacgtgtcca
    14881 tccaggcgca gatcgtcaac ctgctcatcg gactgcagga agaactgggt gtgtcatatc
    14941 tgttcatctc gcacgacatg gccgtggtcg aacgcatcag tcaccgcgtg gccgtcatgt
    15001 acatggggca gatcgtggaa atcggcccgc gccgcgccat cttcgagaac ccccagcatg
    15061 cctacacgaa gaagttgatg tcggcggtgc ccattgccga tccgagccgt cgtcatatgg
    15121 cgcacacgct gctgtcggga gagattccca gcccattgcg ccgtctgggt gacgagccgg
    15181 ttgtcgagcc gctggtcgag tgtggccggg atcatttcgt tgcccgccac gcggtgggtg
    15241 gaggtctggc ttaggggtat ccggttcccg tgcggagcga gtgccggtcg gtcggggcag
    15301 gccctgagag ggagcggttt ccacgtgtcg tggacacacg acatcaagaa ggcgcttaat
    15361 tttattgaac atcagttgga ggagtttcgt catgcttgaa agattcaagc ccgccggtgt
    15421 cgtcatcgcg gctgcgctgg cctttggcgc gttccacgtg ccggtggcca tggctgccaa
    15481 gaatgtcacc gttgcggttc cgtatgccat agactcgctg gacccgtaca acaccaacag
    15541 cacgctgagc atggctgtcg gcaaggccta ctacgaaggg ctgttcgcat tcgacaagaa
    15601 cctgaagatc gtggatttgc tggcgaccgg gtacagcgtc acaccggacg gcctgatcta
    15661 caccatcaag cttcggcacg gtgtcaaatt ccaggacggc acggatttca acgccgcggc
    15721 ggtcaaggcc aacctggatc gcgtcaccaa caaagccaat cacctggccc gctataacca
    15781 gttcaaccgc atcaagtcgg tcgaggtcgt ggatccttac acggtgaaga tcaccctcac
    15841 ggagccgttc tcgccgttca tcaactccct ggcgcatcca gccgccatga tgatatcgcc
    15901 gaccgcgctg aagaaatggg gcaaagacat tgcgttccat ccggtgggta cagggccgtt
    15961 cgaatttgtc gaatggaaac cgtcggagta cctgaaggtc aaaaaatggg atggctattg
    16021 ggacaaaggg ctgccaaaga tagattccct gaccttccgc acggtgactg acaacaacac
    16081 gcgcgccgca gtcgttcaga ccggggaagc gcagttcgtc tacccggtgc cctacgagca
    16141 ggccaacatt ctgaagaaga atcccaagct cgaggtggtg gtgagcccgt cgatctcggt
    16201 caagtatgtg tcgttcaaca tgctgcagaa gccgttcgac aacctgaagg tgcgcgaggc
    16261 catcaactat gcgatcaaca agaaagcgct gatcaaggtg gcttacggcg gctatggcga
    16321 acccatgaac ggcgtggtgc ccacgtccgt cgactatggc gtgaagatga agccctggcc
    16381 gtatgatccc gccaaggcgc gcgaactgtt gaaagaggcc ggctatcccc atggcttcac
    16441 caccacgctg tggtctggct acaacgacgg cacgtctgtc aaggccgttc agttcctgca
    16501 gcagcaattg gcccaggtgg gcatcaaggc atcgctgact gtgctcgaat caggccagcg
    16561 cgtgcagcag gtgcagcagg tgcagaaccc ccaggacgcc aaggtgcgca tgttctatgc
    16621 gggatggtcg tcgtccacag gcgaggcgga ttgggcgctg cggcccttgt tcgactccaa
    16681 ggactggccg cccgtgttga acaacacagc gtattacaag aaccccaagg tggacgagga
    16741 catcgcccag gcgctgctga ccaccgaccg cgccaagaag caggcgctct atacagacgc
    16801 gcaggagcgc atctggaagg atgcgccgtg ggccttcctg gccattccaa gcaatatctc
    16861 ggcacagagc aagaagctga ccggcatgta cgtcatgccc gatggttcgt tctcgttccg
    16921 ggacatcgat ctgaagcaat agcctgaaag gcgcgcgggg ctccggcctc gcgcgctgcg
    16981 ggagtctgga tgttcattta cataatcaaa cggctcgtgg gcatggtcat caccttgttg
    17041 ctggtggcga tggtcgtgtt cctgttcgtg cgcatgctgc cgggcgaccc ggcgcgtctc
    17101 gcggccggtc cggaggccga ccagcagacg gtcaacctcg tgcgccagga attggggctg
    17161 gacaaacccc tgcctgtgca gttcgtccgt tatctagacg gcctgatgca cggcaagatg
    17221 ggtacctcgc tgcgcaccag gcaaccggtg gaaaccgtga tcgccgaacg cttcatgccc
    17281 accttctggc tgactctgct gagcatgctc tggtcagtga ccttcggcat ggtgatcggc
    17341 atcgtgtcgt ccgtgtggcg caatcgctgg ccggaccggc tctgcatgac gctggcggta
    17401 tcggggattt catttccggc cttcgcgctg ggcatgatgc tgatccagat cttctcggtg
    17461 cagctcggat ggctgcccac cgtgggcgcg tcgacgccgg cgcattacat cctgccg
//
LOCUS       DASTNK010000170         4834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1458594, whole genome shotgun sequence.
ACCESSION   DASTNK010000170 DASTNK010000000
VERSION     DASTNK010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458594"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..319
                     /locus_tag="VFR20_12460"
     CDS             56..319
                     /locus_tag="VFR20_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006215900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HEU0231377.1"
                     /translation="MEKLFSWVVLIVVGLVIARILSRQNAARQHMATHARQAPRRGAP
                     PVAAPEAMVRCAHCGIHLPRSEAVLIQGKTWCCEEHAKLGLRQ"
     gene            316..891
                     /gene="ampD"
                     /locus_tag="VFR20_12465"
     CDS             316..891
                     /gene="ampD"
                     /locus_tag="VFR20_12465"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEU0231378.1"
                     /translation="MNRLALDHDGWLEPGPRVRRAPSPNFDTRPAGATPTLLVIHNIS
                     LPPGEFGGPEVVDFFQNRLDYSSHPWLERLRDMRVSAHFFVRRDGGIVQLVSTDARAW
                     HAGVSAFEGRERCNDFSIGVEMEGTDTIPYTDDQYQALTRLNRALCDRHALSAVRGHE
                     HIAPGRKTDPGPSFDWARFGREAGWPRHLLP"
     gene            complement(955..2865)
                     /gene="htpG"
                     /locus_tag="VFR20_12470"
     CDS             complement(955..2865)
                     /gene="htpG"
                     /locus_tag="VFR20_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019248757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEU0231379.1"
                     /translation="MSDSDTSVHASETLGFQAEVKQLLHLMVHSLYSNKEIFLRELVS
                     NASDACDKLRFQAIDQPDLLQGDTDLRIRVDFDKAGRTVTISDNGIGMSRDEAVANLG
                     TIARSGTKEFFSHLTGDNQKDAQLIGQFGVGFYSSFIVADKVSVLTRRADAAETDAVL
                     WESDGQGEFSVTQVEKADRGTSVTLYLREDEDDFLNGWKLREVLRRYSDHISLPVQML
                     KEEWDSEKSEQVKKNEWETVNQASALWTRAKSDITDEQYREFYKHISHDFDDPLAWTH
                     NRVEGRNEYTQLLFVPKHAPMDLWDRDGRRGVKLYVKRVFIMDDAEQLLPTYLRFVRG
                     VIDSADLPLNVSREILQESRDVRAIRDGSAKRILSLLEDLADNKAEQYTEFWGQFGQV
                     LKEGVGEDMANKERIAKLLRFASTRNDSSAQSVSLAEYVAAMKEGQDKIFYVSADSHA
                     AASNSPHLEIFRKKGLEVLLLSDRVDEWMLSYLRDFEGKSLVSVAKGGLDLDKLADEE
                     EKKHQTEVAETFKPLVERLKTSLGDKVKDVRVTLRLVDSPACVVVDENELSPHLLRML
                     KAAGQDAPEVKPILEINPEHAIIARAKGAQDADFGEWALLLLDQAMLAEGAQLADPAA
                     FVKRVNRLLLGA"
     gene            2950..3849
                     /locus_tag="VFR20_12475"
     CDS             2950..3849
                     /locus_tag="VFR20_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0231380.1"
                     /translation="MGIPSGISRDRLRTRAGIACMLLGVLSLSISDASVKSLDGRYAA
                     LQILFLRAGIALPFVIALAWALGGARSLRTRQPVLHTWRGLLNVCGACTFYLGLVHLP
                     LAATTSIAYVAPIFVVLLSALILREPLDPHRLAAVAMGFAGIVIIARPGSAAFQPAAI
                     LPLLTAVTYALMMISARRMDPGHSFPALMFYTVLPQAAFTAITLPWVWQPIASSDWLT
                     LLDISLFSTLGISLISQSFRLAPASAVAPFDYTGLLWATFWGWLIWDQLPDGISYVGM
                     ALIIAAGLSGMWRRAGSGVGKPL"
     gene            3914..3998
                     /locus_tag="VFR20_12480"
     tRNA            3914..3998
                     /locus_tag="VFR20_12480"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3948..3950,aa:Leu,seq:caa)
     gene            4134..4834
                     /locus_tag="VFR20_12485"
                     /pseudo
     CDS             4134..4834
                     /locus_tag="VFR20_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385722.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          860 a         1563 c         1456 g          955 t
ORIGIN      
        1 tggcctacac cggcagccgt ttcgttcttg aagtgcttct gcagcgaggc tgaacatgga
       61 aaaactattc agctgggtcg tgctcatcgt ggtgggcctg gtcatcgcgc gcatcctgtc
      121 gcgccagaac gccgcccgcc aacacatggc cacgcatgcg cgccaagcgc cccgacgcgg
      181 cgcgccaccc gtcgccgcgc ccgaagccat ggtgcgctgc gcgcactgcg gcatccacct
      241 gccccgctcc gaagcggtgc tcatccaggg caagacctgg tgctgcgagg aacacgccaa
      301 actgggcttg cgccaatgaa tcgtctggcg ctggaccacg acggctggct cgaacccggg
      361 ccccgcgtgc gccgggcgcc ctcgcccaat ttcgataccc gcccggccgg cgccacgccc
      421 actctgctgg tgatccataa catcagcctg ccgcctggag agttcggcgg cccggaagtc
      481 gtggacttct tccagaaccg gctggactac tcatcacacc cctggctgga gcgcctgcgc
      541 gacatgcggg tttcggcgca tttcttcgtg cgacgcgatg gcggcatcgt gcaactggtg
      601 tcgaccgatg cgcgcgcctg gcacgccggc gtgtcggcat tcgaaggccg ggaacgctgc
      661 aatgattttt ccattggcgt cgagatggaa ggcaccgaca ccattcccta caccgacgac
      721 cagtaccagg cgctgacgcg attgaaccgc gccctgtgcg accgccacgc cttgagcgcg
      781 gtgcggggcc acgaacatat cgcgccgggg cgcaagaccg accccggccc ctcgttcgac
      841 tgggcgcgct tcggccggga agccggttgg ccccggcatc tgctgccctg acaaaacaag
      901 cgccgcgcaa ggggatctgc agggctgcgc ggccggcaca atcccaccct gcggctacgc
      961 ccccagcaac agccggttga cacgcttgac gaacgcggcc ggatcggcca gttgcgcccc
     1021 ttcggccagc atggcctggt caagcagcaa cagtgcccat tcgccgaagt cggcgtcttg
     1081 cgcgcccttg gcgcgcgcga tgatcgcgtg ctcggggttg atctccagga tgggcttgac
     1141 ctcgggcgcg tcctggccgg ccgccttcag catgcgcagc agatgcgggc tgagctcgtt
     1201 ctcgtcgacc accacgcagg cgggcgagtc caccaggcgc aatgtcacgc gcacgtcctt
     1261 caccttgtcg cccagcgagg ttttcaggcg ttcgaccaat ggcttgaagg tttcggcgac
     1321 ttcagtctgg tgtttcttct cttcctcgtc ggccagcttg tccaggtcca gcccgccctt
     1381 ggcgaccgac accagcgact tgccttcgaa gtcgcgcaga tacgacagca tccattcatc
     1441 gacgcggtcg gacaacagca acacttccag gcccttcttg cggaagatct cgagatgcgg
     1501 gctgttgctg gcggcggcgt gggaatcggc actgacgtag aagatcttgt cctggccctc
     1561 tttcattgcg gccacgtatt cggccaggct gaccgactgc gcagaggaat cgttgcgggt
     1621 ggacgcgaag cgcagcaact tggcgatgcg ctctttgttg gccatgtcct cgcccacgcc
     1681 ttccttgagc acctggccga actgccccca gaactcggtg tactgctcgg ccttattgtc
     1741 ggccaggtct tccagcagcg acaggatgcg cttggccgac ccgtcgcgga tggcgcgcac
     1801 gtcgcggctt tcctgaagga tttcacgcga cacgttcagc ggcaaatcgg ctgaatcgat
     1861 cacgccgcgc acgaagcgca gatacgtggg cagcagttgt tcggcgtcat ccatgatgaa
     1921 cacgcgcttg acgtacagct tcacgccccg gcgtccatcg cgatcccaga ggtccatggg
     1981 cgcgtgcttc ggcacaaaca acagctgggt gtattcgttg cggccctcga cccggttgtg
     2041 cgtccaggcc agcggatcgt cgaagtcgtg tgagatgtgc ttgtagaact cccggtactg
     2101 ctcgtcggtg atgtcggact tcgcgcgggt ccacagcgcg ctggcctggt tcaccgtttc
     2161 ccattcgttc ttcttcacct gctcggattt ctcgctatcc cactcttcct tgagcatctg
     2221 caccggcagc gagatatggt cggaatagcg gcgcagcact tcacgcagct tccagccgtt
     2281 caggaaatcg tcctcgtctt cacgcaggta cagcgtgacc gacgtgccgc gatcggcctt
     2341 ttccacctgc gtgacggaaa actcgccctg cccgtccgat tcccacagca ccgcgtcggt
     2401 ctcggccgca tccgcgcgcc gggtcagcac cgataccttg tcggccacga tgaacgacga
     2461 ataaaacccc acgccaaact ggccgatgag ctgggcgtcc ttctggttgt cgcccgtgag
     2521 gtgcgagaag aattctttgg tgcccgaccg tgcgatggtg cccagattgg ccaccgcctc
     2581 gtcacgcgac atgccgatac cgttatccga gatcgtgacg gtgcgcccgg ccttgtcgaa
     2641 atccacccgg atgcgaaggt cggtgtcgcc ctgcagcaga tcgggctggt ctatggcctg
     2701 gaaacgcaac ttgtcgcagg cgtccgacgc attggaaact agttcgcgca gaaagatttc
     2761 cttgttgctg tacagggaat gcaccataag atgcaacaat tgcttgactt cagcctggaa
     2821 cccaagggtt tcggatgcgt gaacggaggt atcggaatcg ctcataattt gtgctgtttg
     2881 ctggataaag aatgaaaaaa caaccgcgta gccctgaatc ggggccaacc agggcgcatt
     2941 agaacttata tggggatacc gtcgggaatt tcaagagacc gcctgcgcac ccgcgccggc
     3001 atcgcctgca tgctgctggg tgtgctgtcg ctgagcatca gcgacgcctc ggtcaagtca
     3061 ttggatggcc gctatgccgc cctacagatt cttttcctgc gcgccggcat cgccctgcca
     3121 ttcgtcatcg ccctggcgtg ggccttgggc ggggcgcgat cattgcgcac ccgccaacca
     3181 gtgctgcaca cctggcgcgg cctgctgaac gtctgcggcg cctgcacctt ctacctgggc
     3241 ctggtgcacc tgcccctggc cgccaccacg tccatcgcct atgtcgcccc catcttcgtc
     3301 gtcctgctgt cggcgctgat cctgcgcgaa ccattggatc cgcaccgact ggcggccgtc
     3361 gccatggggt tcgccggcat cgtcatcatc gcccgccccg gtagcgcggc tttccagccc
     3421 gccgccatcc tgccgctgct gaccgccgtc acctacgcct tgatgatgat cagcgcccgg
     3481 cgcatggacc ccggccacag cttcccggcc ctgatgttct acaccgtgct gccccaggcc
     3541 gctttcaccg ccatcaccct gccctgggtc tggcagccca ttgcgtcgag cgactggctc
     3601 acgctgctgg acatcagcct gttcagcacc ctgggcatca gcctgatctc gcaaagcttc
     3661 cgcctggcgc ccgcgtcggc cgtggccccg tttgattaca ccggattgct gtgggccacc
     3721 ttctggggct ggctgatctg ggaccagttg cccgacggca tcagctacgt gggcatggcg
     3781 ctgatcatcg cggcggggtt gtcgggcatg tggcgccggg cgggcagcgg ggtgggcaag
     3841 cccctgtaag agtgcgtata attgcgtctc ttctccacac ctcggcgcgg cagccccggg
     3901 gtgatccccc agggcccggg tggtgaaatt ggtagacgca ggggactcaa aatcccccgc
     3961 cgcaaggcgt gccggttcga ttccggcccc gggcaccagc ttaaaatacg caatgtttat
     4021 aagtgtttag ctgggttcag acctctaccc caagactggg ggattttcca agattgcgag
     4081 tcaggtacag tttcggtaca gtggtgaagc gttgagcatc atccggagcc tgaatggcca
     4141 ctatcgtcaa gactccttcc ggcacctgga aggcattaat ccgcaaaact ggctggcccg
     4201 ctaccgccaa aactttccgc actaaaagag acgccgaaga ctgggcgcgc cgtactgaag
     4261 atgaaatggt acgcggggtg tacatacacc gtgcgcctgc tgaacggatg actattgagg
     4321 cggcgctgaa gcggtatctg cgagaagtta ccccaactaa gcgcgcatcg acccagattg
     4381 gcgaacataa aaaggccctg gcactgatca agcatctggg taaatattcc ctggctggct
     4441 tgagcgctga tattgttgcc cagtaccgtg atgcgcggct ggccgatgat gaagatgctg
     4501 aaggtaagcg agtaccgcgt agcaataaca cggtacgcct ggagctagcc ctacttggcc
     4561 acctattcac gattgcgatc aaggaatggg gcattgggct gccgttcaac cccgtgtcta
     4621 atattcgccg cccggctccg ggcgcggggc gtaaccgacg gctgacaccg gcagagaata
     4681 aacggctact gatggcagtg gatgcctatt caaatccaat gtttagctgg atagtactga
     4741 ttgcgttgga aacaggcatg cggctgtcgg aaattgctgg cgtgcgggtt ttggccaagt
     4801 ggatatgcag aagcgggttg tgcgactgga tatc
//
LOCUS       DASTNK010000171        10070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_535986, whole genome shotgun sequence.
ACCESSION   DASTNK010000171 DASTNK010000000
VERSION     DASTNK010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10070
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535986"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1049
                     /locus_tag="VFR20_12490"
     CDS             96..1049
                     /locus_tag="VFR20_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0231381.1"
                     /translation="MQRHREPIETYLLRVLQTLLTEQSVSRAAMRLGLSQPAISNSLK
                     RLREITGDAILVRGKSGMVPTERGRELLAHATEALVAIDRITHSPVEFSPQTCTREFH
                     LGAPDYLDALFLPNIAEIVFRNAPQAKLIVHPINADYNYEEALENGTLDVVIGNWREP
                     PPHLHMSRLFDDEVICMVGSQHFLADKGISLQHYLELHHLAPTPYVSERTSFIDGCLA
                     EHGLRRHIHMTIPYFGLVPYVLMSTDLVFTTGRQFAQHYTQYLPIKVLPSPFKFPPMR
                     FYQLWHKRTHGAADITWLRRRIADVATRLQSGLRTPPPDQT"
     gene            1217..1456
                     /locus_tag="VFR20_12495"
     CDS             1217..1456
                     /locus_tag="VFR20_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231382.1"
                     /translation="MRINQTARQPVAAPLRTTGESAIPIQGPAGSFRKKFPDKTLDHL
                     AASDTAAAGTHGDNPPTRRRAILRHAVPVGNLKHA"
     gene            1543..2901
                     /locus_tag="VFR20_12500"
     CDS             1543..2901
                     /locus_tag="VFR20_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU0231383.1"
                     /translation="MAAPIAPATGLRQKLEGLFDMAARGTSLRQESIAGLTTFLTMVY
                     SVFVVPGMYGEAGFDKSAVFIAVCLVSAFGSLLMGLWANLPIAVGCAISLTAFTAFDL
                     VLGQKLPPTVVLGAIFLMGIVFTSISVTGVRSWILRNLPAGIAHGTGVGIGLFLLLIA
                     ANGVGLVVKNPGAGLPVSLGHLTTLPALATIVGLAAIFGLERRRVPGSILIVIIALSA
                     FGLAFDPHVKFSGLAAIPSLSSAGHGTLIGAMDIKGALTVAVIPSVLALVMTAVFDAT
                     GTIRAVAGQAGQLDEKGQIINGGRALASDSISSIVSAFLGGAPAAAYIESASGVAAGA
                     KTGFAAAVVGVLFLAMLFLAPLAGLVPSYATAPALMYVGLLMLGSISKLHMEDMVDAM
                     AGLVCAVFIVLTCNIVTGIMLGFSTLVLGRIVSGEFRKLNVGTVSIALALAVFYLGGW
                     AI"
     gene            3125..3652
                     /gene="uraD"
                     /locus_tag="VFR20_12505"
     CDS             3125..3652
                     /gene="uraD"
                     /locus_tag="VFR20_12505"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEU0231384.1"
                     /translation="MMPLDTPVLTLTDLSRMPREEFTTRLGAIFEHSPWIAEGAWASR
                     PFADVDQLHDAMLAVLNAANDADRLALICAHPELAGKEAASGTLTHASTNEQRGAGLD
                     QCSANELAHLRALNAAYRKRFGFPFVIAVKGLSRHQIMDAIECRLENPRETEFAACLT
                     EIGKIARFRLDTLFA"
     gene            3670..4182
                     /locus_tag="VFR20_12510"
     CDS             3670..4182
                     /locus_tag="VFR20_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HEU0231385.1"
                     /translation="MTTDTLTPVTLVAEPLTRDAFSPFGDVIEVSDAVKHFPINDGNT
                     ERYHDLANIDPGAGGRVIVSIFRGLPRRLPFELTMMERHPRASQAFIPISGRPYLVAV
                     APIGNAPTIQDIRVFHCKASQGVNYATGVWHHPLMALENTSDFLIIDRSGPGDNCDVI
                     ELEQRGVVVL"
     gene            4400..5128
                     /locus_tag="VFR20_12515"
     CDS             4400..5128
                     /locus_tag="VFR20_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0231386.1"
                     /translation="MPIDATLSETETGDPGLDTLIAALTQDGENQAQIAYRVLEEMIV
                     TMKLPPGSKISEKRLNRALGLGRTPLREALQRLANEGTVKILPRAGVIVSDIDMVDQM
                     NMIEVRRELEKIIAGRAARLATKAERDMFKQLAQRFDRAASENDENIFIPADREFNSL
                     SVKAAQNKYVTYAITPIGAQTRRFWYLYFRRFGDLAHTAKLHAVIARCIATGDENGAR
                     KASDALLDYVEEYTHKTLCCLSGI"
     gene            complement(5262..6863)
                     /locus_tag="VFR20_12520"
     CDS             complement(5262..6863)
                     /locus_tag="VFR20_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEU0231387.1"
                     /translation="MLNSTLSTRGMAVAPHSLAAQAAMSILRRDGGNAIEAMVAAAAA
                     IAVVYPHMNGIGGDGFWVISEPGKPVMAIEACGTAGGGVSTQFYQDRGLEAIPVRGPL
                     AANTVAGTIQGWQEALNISASWGGRLPLARLLEDAIHYAETGIPVTCSQHASTSAKLQ
                     ELAEQSGFSDTFLVDGNVPIAGSRFVQPRMAKTLRTLVVQGLDSFYRGDLARMIADDL
                     AEIGSPIGLRDLNGYHARTVPPLRLKHRLGDIYNTAPPTQGVVSLAILGILDRLNIGQ
                     YEEGSVEYVHLMVEATKQAFLMRDRYVCDPIHMSVEPQACLSDAFLAPYVKAVSLEKA
                     LPWGAGKGPADTIWMGVIDDAGRAVSFIQSIYHEFGSGTVLPRTGINWQNRGCSFSLA
                     KGDLHELMPGKKPFHTLNPALARFSDGRIMVYGTMGGDGQPQTQATVFTRHAVFGQPV
                     QQAITAPRWLLGRTWGESSESLKLESRFKPEIIDGLRALGHEVDVVGEFDEMMGHAGA
                     IVRDAGTGILEGGVDPRSNGVVAGY"
     gene            complement(6969..8330)
                     /gene="oxlT"
                     /locus_tag="VFR20_12525"
     CDS             complement(6969..8330)
                     /gene="oxlT"
                     /locus_tag="VFR20_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HEU0231388.1"
                     /translation="MHSIRPKDASFKRTRWLQLVLGLVAMMAISSPQYVWALFTSDLT
                     RMLGVSLAEVQVTFALLIIIQTFLAPFQGFLIDRFGAKLLLSCGAVLTGLSWILASKV
                     TSIWGLYLAYGVLGGLGTGIIYIGVIGLMVRWFPDKRGFAVGMVAAGYGFGAALTTFA
                     ISDSIKSIGAPDTLVVFGWIIGVVGLLAVLGLRRPREGELRELAQLVSSARAAKAGDF
                     SAPTVGNRDYTPREMVREPVFWVMYVMFVMMATSGLMVISQIGAFAKDIHVANALVFG
                     MAALPLALTLDRFTNGLTRPLFGWVSDHIGRENTMFVAFGLEGVAMSIWLAASSDPLL
                     FALLSGVVFFGWGEIFSLFPATLTDIFGTKYATTNYGLLYMAQGVGSVLGGPLAAMMH
                     QTTHSWTAVFVVVIAMNFITALMAIFVLKPMRHAVHGRVTTSGVTAFQMKQPALMPLT
                     PPD"
     gene            complement(8668..9597)
                     /locus_tag="VFR20_12530"
     CDS             complement(8668..9597)
                     /locus_tag="VFR20_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0231389.1"
                     /translation="MDRYTAIRSFVLVAEKGSFAAAALAEGVTPVVMGRRLNELERRL
                     GVQLVHRSTRGLALTDLGEQFLESCQQLLQDFDAAEGSVSRGRSTVRGHLVVSGPAAF
                     GRRHIAPHAPEFRRRHPDLRLSFNFTDSVVDLVREGYDVAVRIGEVRDPNYVAVRLFP
                     NRRVVCGTPGYFERNGVPRVPEDLARHNCLAFNLQGGQQRGWIFERDGKPFAVRVDGD
                     LACNDGELLYAWVKQGLGVGWRSTWEIQAELKRGELVTVLDEFALPRYDIQAVYPQQR
                     YLPAKVRFFIDYLREVYQRPGYWEGEAVSWGGS"
     gene            9876..>10070
                     /locus_tag="VFR20_12535"
     CDS             9876..>10070
                     /locus_tag="VFR20_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU0231390.1"
                     /translation="MARMRAVDAAAAVLEKEGIRTLFGVPGAAINPLYSALRKNGQFR
                     HILGRHVEGASHMAEGFARAR"
BASE COUNT         2089 a         3525 c         2699 g         1757 t
ORIGIN      
        1 aatgatgacc attatttcga tatggagaca agccgggccg tgggcactcg cgattccaat
       61 ggacaacgcg ccggggccaa cccgataata ccggcatgca acgacaccgg gaacccatcg
      121 aaacctatct actgcgcgtc ctgcaaacgc tgctcaccga gcaaagcgtt tcacgcgccg
      181 ccatgcggct ggggctttcg cagcccgcca tcagcaattc gctcaagcgg ctgcgggaaa
      241 tcaccggcga cgccatcctg gtgcgcggaa aatcgggcat ggtgcccacc gagcgcggcc
      301 gcgagctcct tgcccacgcc accgaggcgc tggtggccat agaccgcatc acccactcgc
      361 ccgtcgaatt ctcgccgcag acctgcaccc gggagttcca tctgggcgca ccagactacc
      421 tggatgccct cttcctgccc aacatcgccg aaatcgtgtt tcgcaacgcg ccgcaggcca
      481 agctcatcgt gcaccccatc aacgccgact acaactatga agaggcgctc gagaacggca
      541 cgctggacgt cgtcatcggc aactggcgcg aaccgccgcc gcacctgcac atgtcgcgcc
      601 tgttcgacga cgaggtcatc tgcatggtcg gcagccagca tttcctggcg gacaagggca
      661 tttcgctgca gcattacctg gaactgcacc acctcgcccc cacgccctac gtgtcggaac
      721 gcaccagctt catcgacggc tgcctggccg aacacggcct gcggcgccac atccacatga
      781 ccattcccta cttcggcctg gtcccgtacg ttctcatgtc caccgacctg gtgttcacca
      841 ccggccggca attcgcccag cactacacgc agtacctgcc catcaaggtg ctgccgtcgc
      901 cgttcaagtt cccacccatg cgcttctacc agctctggca caagcgcacg catggcgcgg
      961 ccgacatcac ctggctgcgc cggcgtatag ccgatgtggc cacccgcctg cagtccggac
     1021 tgcgcacacc cccgcccgac caaacgtaat cagtgattac ccgaacgccg ccaccatcat
     1081 ctcctatggc ggcggcgtga ctatcatcgc gccttgatac cgaccatcac gcctgactga
     1141 aagtccggcg cggcaagcgt gcctagaatg gcccggcgtg ttgtgtggct ggttcgaaca
     1201 ctcaacgaat ttgaatgtgc gaatcaacca aacagcgcgc cagccggtcg ccgcgcccct
     1261 gcgcacgacc ggcgaaagcg cgatccctat ccagggcccc gccgggtcat tccgcaagaa
     1321 attccccgac aagacactgg atcacctggc ggccagcgac acggcagccg cggggacgca
     1381 cggagacaac ccgccgaccc gccgccgcgc catccttcga catgcggtgc ccgtcggcaa
     1441 cctcaagcac gcctgatgac ggccctagac ggctcgtttc agcccgctga taaaggagct
     1501 tgcatcatga ccgaccaaga actcgccctc ggccgcgagg ccgtggccgc ccccattgcc
     1561 ccagccacag gcctgcggca aaaactggaa ggcctattcg acatggccgc gcgcggcaca
     1621 tcgctgcgcc aggaatccat cgccggcctc accaccttcc tgaccatggt gtactcggtc
     1681 ttcgtcgtac ccggcatgta tggcgaagcg ggcttcgaca agagtgccgt cttcatcgcc
     1741 gtctgcctgg tcagcgcctt cggctccctg ctgatggggt tgtgggccaa cctgcccatc
     1801 gccgtcggct gcgccatctc gctgaccgcc ttcaccgcct tcgacctggt gctcggccag
     1861 aaactgccgc ccaccgtggt gctcggcgcc atcttcctga tgggaatcgt gttcacgtcg
     1921 atttccgtca cgggcgtgcg ctcctggatc ctgcgcaatc tgccggccgg catcgcacat
     1981 ggcacggggg tgggcatcgg gctgttcctg ctgctgatcg ccgccaacgg cgtcggcctg
     2041 gtcgtgaaga accccggcgc cgggctgcct gtctcgctgg gacacctgac caccctgccc
     2101 gcgctggcca ccatcgtcgg cctggccgcc atcttcgggc tggagcgccg ccgtgtgccg
     2161 ggcagcatcc tgatcgtcat catcgcgctc tccgcgttcg gcctggcgtt cgacccgcac
     2221 gtgaaattca gcggtctcgc cgcgatcccc tcgttgagct cggccggaca cggcacactg
     2281 atcggcgcga tggacatcaa gggtgcgctg acggtggcgg tcattcccag tgtgctggca
     2341 ttggtgatga cggcggtctt cgacgctacc ggcaccatcc gcgccgtggc gggccaagcc
     2401 ggccagctcg atgagaaagg ccagatcatc aacggcggcc gcgccctcgc gtcggactcc
     2461 atcagttcga tcgtgtcggc cttcctgggc ggcgcgcccg ccgcggccta cattgaatcg
     2521 gcctccggcg tcgccgccgg cgccaagacc ggcttcgcgg cggccgtcgt cggtgtgctg
     2581 ttcctggcca tgctcttcct ggcccccctg gccgggctgg ttccttcgta cgccaccgcc
     2641 cccgcgctga tgtacgtcgg cctgctgatg ctgggcagca tctccaagct ccacatggaa
     2701 gacatggtcg acgccatggc cgggctggtg tgcgccgtgt tcatcgtgct cacctgcaac
     2761 atcgtgaccg gcatcatgct cggcttcagc accctggttc tgggtcgcat cgtctcgggc
     2821 gaattccgca aactcaacgt cggcacggtc tcgatcgccc tcgcgctggc cgtgttctac
     2881 ctgggcggct gggccatctg atccggtcaa ggcacaagcc gccggttgat acgcggcggc
     2941 ttatacctgt tatgcggttt tcccgactgc catccccgcc tggaagccgt acacttgaca
     3001 caacggatgg acgctccatg cgggttcgcg caccggcgga gacacgcggg cggaattctc
     3061 ttgatgctcc cggcgcggcg cgagccgcca cgttcaaccc gacttcatcc acagactcga
     3121 caccatgatg ccactcgaca cgcccgtcct gaccctgacc gacctctccc ggatgccgcg
     3181 ggaagaattc acgacacgcc tgggcgccat cttcgaacac tccccctgga tcgccgaagg
     3241 cgcctgggcc agccgcccat tcgccgatgt cgaccagttg catgacgcca tgctggccgt
     3301 gctgaatgcg gccaacgatg ccgaccgcct ggccctcatt tgcgcccacc ctgaactcgc
     3361 cggcaaagag gctgccagtg gcacactcac gcacgcctcc accaacgaac agcgcggcgc
     3421 cgggctcgat caatgcagcg ccaacgaact ggcgcacctg cgcgcgctca acgccgccta
     3481 tcgcaagcgc ttcggttttc cgttcgtcat cgcggtcaag gggctcagcc gtcatcagat
     3541 catggacgcc atcgaatgcc gccttgaaaa cccccgcgaa acggaattcg ctgcctgcct
     3601 gacggaaatc ggcaagatcg cccgcttccg gctcgacacc ttgtttgctt gaaaccggga
     3661 ccgcccacca tgaccaccga cacgctcacg ccagtcaccc tcgtcgccga accgctcacg
     3721 cgcgacgcct tcagcccgtt cggcgacgtc atcgaagtgt ccgacgcggt gaaacacttc
     3781 cccatcaacg acggcaacac cgagcgctac cacgatctgg ccaacatcga ccccggcgcc
     3841 ggcggtcgcg tcatcgtttc cattttccgt ggcctgccgc gccgcctgcc gttcgaactc
     3901 accatgatgg aacgccatcc gcgcgccagc caggcgttca tcccgatttc cggccggccg
     3961 tacctggtgg cggtggcgcc aatcggcaac gcccccacca tccaggacat ccgcgttttc
     4021 cactgcaagg catcgcaagg cgtgaactac gcaaccgggg tctggcacca ccccctcatg
     4081 gcgctggaaa acacctccga ctttctcatc atcgaccgca gcgggccggg cgacaactgc
     4141 gacgtcatcg agctggagca gcgcggcgtg gtggtgcttt aaacaacgcc tttgcgcggg
     4201 aaggaactgg attccgcacc tacccggcgg tgagtcgcgc accagagtgc gggcccggta
     4261 ctcaatgtcg agatcaacgc ggctcgtccg tatcggcacg aacctgttct tccgggaaga
     4321 aggctactac ccctcatcaa atcaaataag gtcataatta ggcgttcgga cgtcacgtcc
     4381 tcaagcgcca gaaacccgta tgcctatcga tgccaccctc tcagaaacag aaaccggcga
     4441 cccgggcctt gacaccctga tcgcagcatt gacacaagac ggcgagaatc aggcccagat
     4501 agcctaccgg gtgctagaag aaatgattgt gacgatgaaa ttgccaccgg gcagcaagat
     4561 ttccgagaag aggctgaacc gtgcactcgg cctggggcgc acgccgcttc gggaagccct
     4621 gcagcggttg gcgaacgaag gcaccgtcaa gattctgccc agagccggcg tcattgtttc
     4681 cgatatcgac atggtcgacc aaatgaacat gatcgaggtg cgccgggagc tggaaaaaat
     4741 cattgccggc cgcgcggcgc gcctggcaac caaggcggaa cgcgacatgt tcaagcaact
     4801 ggcacaaaga ttcgaccgcg cggctagcga aaatgacgag aacatcttta ttccggctga
     4861 tcgcgaattc aactccctct cggtcaaggc cgcacagaac aaatatgtga cctacgccat
     4921 cactcccatc ggcgcccaga cgcgccgttt ctggtatttg tacttcaggc gttttggcga
     4981 tctggcccat accgccaagc tgcacgccgt catcgcgcgc tgcatcgcca cgggtgacga
     5041 aaacggcgca cgcaaggcct ccgacgcact acttgactac gtcgaggaat acacgcacaa
     5101 aaccctctgt tgcctgagcg ggatatagaa aactcgttta caagaagacc cgccgaggct
     5161 tcacgcgtag ggtagcccgc cgctctcgca tgagcgaatg gctttgggta cacggcttta
     5221 cacgcgaaac gagccgccgc cgacgacatc gttaccaagg ctcaataacc ggccaccacc
     5281 ccattgctgc gtgggtcgac gccaccttca agaatgccgg tgccggcatc ccgcacgatg
     5341 gcaccggcat ggcccatcat ctcgtcgaac tccccaacca cgtccacctc atggcccaac
     5401 gccctgagtc cgtcgatgat ttcaggcttg aagcggctct cgagcttaag tgattccgag
     5461 ctttcccccc acgtgcgccc cagcagccag cgcggtgccg tgatggcctg ctgcacgggc
     5521 tggccgaaca cggcatggcg agtgaaaact gtcgcctgtg tctgcggttg cccatcaccg
     5581 cccatcgtgc catacaccat gatccggcca tctgaaaagc gggcgagggc cggattgagg
     5641 gtatgaaatg gcttcttacc cggcatcaat tcatgcaaat cccccttggc cagagagaac
     5701 gagcagccgc gattctgcca attgatgccc gtgcgaggca aaacggtgcc acttccaaac
     5761 tcgtgataga tgctttggat gaacgacacc gcccgtcccg cgtcatcaat cactcccatc
     5821 catatggtat cggccggccc cttcccggcg ccccatggga gcgctttttc aagcgaaaca
     5881 gccttcacgt agggtgccag gaacgcatcg gacagacacg cctgcggctc gacagacatg
     5941 tggatcggat cacatacata tcggtcgcgc atcaagaagg cctgcttggt cgcctcgacc
     6001 atcagatgga cgtattccac actgccttcc tcgtattgac cgatattcag acgatcaaga
     6061 atgcccagga tcgccaacga cacgacgcct tgcgtaggcg gcgccgtgtt gtatatgtct
     6121 cccagacggt gtttgagacg caacggcggc acggttcttg cgtggtaacc attcaggtcg
     6181 cgcaacccga tcggactgcc gatttcggcc aaatcgtcgg caatcatcct ggcgagatcc
     6241 ccacggtaaa aactgtccag cccctgcacc acaagtgtgc gcagggtctt ggccatgcgg
     6301 ggctggacaa aacgactgcc cgcgatggga acgttgccgt caaccaggaa cgtatcggaa
     6361 aacccggact gctcggccaa ttcctggagc tttgcggacg ttgaggcatg ctgcgagcag
     6421 gtgaccggaa tacctgtttc cgcataatga atggcatctt ccagcaatcg cgcaagcggc
     6481 agacggccgc cccaggacgc gctgatgttc aatgcctctt gccacccctg gatcgttccg
     6541 gccacagtgt tggcggccaa tgggccgcga accggaattg cctccaatcc ccgatcttga
     6601 tagaactgcg tggacacgcc tccccccgcc gtaccacagg cctctatggc catgaccggc
     6661 ttgccgggct ccgaaatcac ccagaatccg tccccaccga tgccgttcat gtgtggatac
     6721 acgaccgcta tggcggccgc agcggcaacc atggcttcga tggcattgcc gccatcccgc
     6781 ctcaagatcg acattgccgc ctgcgcggcc agggaatgtg gagcgacggc catgccgcgc
     6841 gtggacagtg tggaattcag catcgtatcg tgatccaacg gaagagaaat ggatgaagag
     6901 tggaaagcgg ctaagagcca gagtggtgtc ggtccagccc ctcatcattc gggccagatc
     6961 acaaaccctc aatccggagg ggtaagcggc atcagtgcag gctgtttcat ctgaaacgcg
     7021 gtcacaccac tcgtcgtgac cctgccatgg acagcatgac gcatgggttt caacacgaat
     7081 atcgccatca gggcagtgat gaaattcatc gcgatcacca ccacgaacac cgccgtccat
     7141 gaatgcgtcg tctgatgcat cattgcggcg agtgggccac ccaggacaga ccctaccccc
     7201 tgcgccatat acagcaagcc ataattggtc gtcgcgtatt tcgttccgaa aatatccgta
     7261 agcgtggctg ggaacaggga aaatatttcg ccccaaccaa agaaaaccac tccggacaac
     7321 aatgcgaaca gcaacggatc actgctggcg gccagccaga tggacatcgc cacaccctcc
     7381 aatccgaatg ccacgaacat cgtgttttca cggccgatat gatccgacac ccagccaaac
     7441 agcggccgcg taaggccatt ggtgaaacga tccagcgtca acgccaatgg cagcgcagcc
     7501 atgccaaaca ccagggcgtt cgccacatgg atatccttcg caaacgcccc gatctgcgaa
     7561 atcaccatca ggccggacgt cgccatcatc acgaacatca catacatcac ccagaacacc
     7621 ggctccctga ccatctcccg gggggtgtag tcacgatttc ccacggtggg cgccgaaaaa
     7681 tcgccggctt tagcggcccg ggccgacgac acgagctgcg ccagttcccg caattccccc
     7741 tcccgcggcc gacgcaggcc gagtaccgcg agcaagccca cgacgccgat aatccatcca
     7801 aagaccacca gcgtatcagg cgccccaatg gatttgatac tgtcggaaat ggcaaacgtt
     7861 gtcagcgccg cgccgaagcc gtacccggcc gccaccattc cgacggcaaa cccgcgcttg
     7921 tcgggaaacc accgcaccat caagccgatg acgccgatat agatgattcc cgttcccagc
     7981 ccacccaaca caccgtaggc cagatacagg ccccagatgc tcgtgacttt cgaggccagt
     8041 atccaactca accctgtcag aaccgcccca caggaaagca gcagtttcgc gccaaatctg
     8101 tcgatcagga aaccctggaa cggggccaga aaagtctgaa taataatcag aagggcaaac
     8161 gtcacctgga cctctgccag agaaacgccc aacatccgtg tcagatcgga cgtgaacaac
     8221 gcccagacat actgcgggct cgatatcgcc atcatggcga ccaaccccag aacaagctgc
     8281 aaccatcttg ttctcttgaa agaagcatcc ttcggcctga tagagtgcat cgctgtctcc
     8341 tagtgagcta tgtctatttt cgacacgcca ggcaagtttt caccggtctg cttcctgcct
     8401 attacatttt tgtttatcac tgacatgtca gttttagaaa ctaaacgctc tagccatttc
     8461 ctgtcaagaa aaaccatgcg gacacaatgc aattattgct acaaattaat tcccgcctcg
     8521 aggcatccac aaacaggcgc ccgtatgcgc cgccccgttc cggcctaggc caagctccga
     8581 tctgggtggc ccaattgatt tgttctggct cttaaacgca gaaacggcgg gaccgaagcg
     8641 ccacccccac ttggcaaaaa cacgtcctca cgaccccccc cacgacaccg cctccccctc
     8701 ccaatacccc ggccgctggt acacctcgcg cagataatca atgaaaaacc gcaccttcgc
     8761 cggcagatac cgctgctgcg gatacaccgc ctggatgtca tagcgcggca gcgcgaactc
     8821 atccagcacc gtcaccagtt ccccccgctt cagctccgcc tggatctccc acgtggaccg
     8881 ccagcccacc cccagcccct gcttcaccca ggcgtacagc aactccccgt cattgcacgc
     8941 cagatccccg tccacccgca ccgcgaacgg cttgccgtca cgctcgaaga tccagccccg
     9001 ctgctgcccc ccctgcagat tgaacgccag gcagttgtgg cgcgccaggt cctcgggcac
     9061 acgcggcacc ccgttgcgct cgaaataccc cggcgtgccg cacaccaccc gccggttcgg
     9121 aaacaaccgc accgccacat aattggggtc gcgcacctcg ccaatgcgca ccgccacgtc
     9181 atagccctcg cgcaccaggt ccaccacgct gtccgtgaaa ttgaacgaca ggcgcagatc
     9241 agggtggcgc cgccggaact ccggcgcgtg cggcgcgatg tggcggcgcc cgaacgccgc
     9301 cggccccgac accaccagat gtccgcgcac cgtgctgcgc ccgcgactga cactgccctc
     9361 ggccgcatcg aagtcctgca gcagttgctg gcagctttcc aggaactgtt cgcccagatc
     9421 ggtgagcgcc agcccgcgtg tggatcgatg caccagttgc acccccaggc ggcgttccag
     9481 ctcgttcagg cggcgcccca tcaccaccgg tgtcaccccc tcggccagcg ccgccgccgc
     9541 gaagctgcct ttttccgcca cgaggacgaa actgcggatt gccgtataac gatccatcac
     9601 atacctttcc taacgagacc gaaccgcgcc ggccaagatc cacccttgcg ccaacaacgc
     9661 gcgaacctgc caaagaatcc gaaaaatggc tttgacatca agccatttac gcctcattca
     9721 actcatcaac atcgaacccg cttatctggg cgtcgcaccc agaacatact aatagtatcg
     9781 acagaaacga tgatggcacg tcttcaaaga cctggcaaac ctctctattc tggactccgt
     9841 caaagaattc aaattcatgc actgaaggag caatcatggc cagaatgaga gctgtcgacg
     9901 ccgccgccgc ggtcttggaa aaagaaggta tccgcaccct gttcggcgtg ccgggcgccg
     9961 ccatcaaccc cttgtattcg gcactgcgca agaacgggca attccgccac atactcggcc
    10021 gccatgtcga gggcgcctcg cacatggccg aaggcttcgc ccgcgcccgc
//
LOCUS       DASTNK010000172        21122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1091950, whole genome shotgun sequence.
ACCESSION   DASTNK010000172 DASTNK010000000
VERSION     DASTNK010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 21122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21122
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091950"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..252)
                     /gene="ffs"
                     /locus_tag="VFR20_12540"
     ncRNA           complement(155..252)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFR20_12540"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(280..3648)
                     /locus_tag="VFR20_12545"
     CDS             complement(280..3648)
                     /locus_tag="VFR20_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU0231391.1"
                     /translation="MSIDKRPSDLEVRIAAVDPSRSFLVQAPAGSGKTELLTDRILAL
                     LATVERPEEIVAITFTRKAASEMHERVLKKLHAARAVVAAEKRGDAEAGRNIPAHQRR
                     SLALARRALEHDDTKKWNLLQYPARLSIRTIDSLCAHLVRAMPWLSHLGGLPKVSDRS
                     GAHYLAAAQATLAMVDDEPVVAALLDHLDVDGRVAQELLALGLARRDQWLPLLDAGDD
                     AALLLANLELAQEEDLRRLSDAMPMGWAGDVGASVAAAARHLADQGGKLDLGPLEDWD
                     GTPFGTDPSIDGPRWRALADALLTAKGELRAKVNKSQGFAPGSPHKQAFEEWLARARP
                     DAGAWAPSLQAARCAPFSGYTDEQLATLNAMLGVLRLASAQLQLRFAETGEVDFIEIA
                     QRAVQALGGADDPSELLLKLDASVKHILVDEFQDTSHSQIVLLESLTAGWSPGDGRTL
                     FLVGDPMQSIYRFRKAEVGLFLKVRDSGLTNVTPEFLRLTDNFRSDQGIVDWVNRTFK
                     PLFPPADDAALGAISYTGSRAFHEAGDGLAVRFHPVWQSGEEDGPDTDSRADSHTRQT
                     ESLVLGLVRDALANRDAESAHPVAILVRARNHLEGVLRLLRQEGVPCRAVELEALKSR
                     QVIEDLVQLARALSHDGDRLAWLSVLRSPVCGMTLSSLHALFGSDHVSAVPFLLARWL
                     EGGGVPTSILSTDEARRLRRAATILLDAGNRSGAVPFAAWLEQCWQRLGGSEVYASVE
                     DQSDAERFFRLIETLAPYGNPDVTELDARLDGLYASSGGDGAAVEVMTIHKSKGMEFD
                     EVVLMGLHRRPATDRSPLVRFELDGDRILLGPIKPRAQDGSDPVSDYLARREKQRADY
                     ESDRLLYVAATRARRRLHLVGLVRVEDGAARPPTSGSLLGRLWDHLDCGEPTFPSLDA
                     GGTPADRHRDLLRLALADLPEPVTAASARPGKHWEWRAEPGDEAAIGTVAHAWLEYLG
                     GHGGGAQWTADNVEQAVPAFRTQLSRAGVREANLAAAADAVRATLAATLASGRGRWLL
                     SVARGHREWSLVDTAGRVSVIDLAISSEEGWLVVDYKTGMPGQEESAEAFAARMRERY
                     AGQMERYRQAVAALDGRPVRAALYFPRADLWIDC"
     gene            complement(3650..6415)
                     /locus_tag="VFR20_12550"
     CDS             complement(3650..6415)
                     /locus_tag="VFR20_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEU0231392.1"
                     /translation="MILDPDASISFDELVEDVLRVGQNADDILIVTVNNRYARRLVTV
                     LAPRLGAGRAAAPLPGILPFTAWLRALGGQACFAPGGVAPARRLDTFASRRLWERVIA
                     RDEADRVLLDIAQAARHALDADRLVSDGYAETLPGEETDEYRRFQVWRRHYRAALADL
                     DAEDDNLEYERVCGAAESGALALSFQTLVLAGFAALSPRQTRLMAALREAGVRICALH
                     PDVGPACRVERVVADEPDAEWRLAAQWAADSLERRPAGRFAIIAARLDSDVVLAHRVL
                     RDALRVPADTPRLEPGIDHAVDALNASLRPVRPYDVAVARPLGEWVHARAALAWLRVL
                     SSFSEQDACSVDTLGAALLAGCCAGASTESSARAALDVAWRRRGTMVVTVKEFVERLT
                     VTAPRLAASWTDWKSTRRGAPTETTVGAWVDLFRQWLRRIGFPGDAALDSHAYQVVEA
                     FESALANLARQETVLGCLTASAALTLLRRLLDETLFQPQRDPSSRLEVLGFLESEGGR
                     WDGVWVLGLTDDVLPAVPGPNPLIPLGALRRAQAPRATPDRELEWARTMVASFLASAP
                     EVWFSHARRDGERDLRASPFFEASPTHPEGAPIASPSAKLVVTQVARRPVRGCVLDRL
                     VDDNGPALVPGDKTRGGIGVIDTQARNPLWAFVKYRLGASDLPGYASLHDRNARGIFL
                     HKAIELVWRLLDNQEGLLRARDDAMLDHLVEQATAQAGDECLRDYGPALKALEVLRGR
                     TVLEAWLALECERPPFTVAALEQDCAWTFGPLDLSLRIDRVDRLADGRMAIIDYKSGL
                     GRLDPKSDWMRERPIGLQLPFYAAVLAERTGQVAALVLARLHARKVEARGLADTDCGL
                     PGLASPADWPLYADHSWERLMDDWRRAIEGLADEYAEGRAVNEIRNSADLDYCDARPF
                     LRLNEDR"
     gene            6546..7379
                     /locus_tag="VFR20_12555"
     CDS             6546..7379
                     /locus_tag="VFR20_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEU0231393.1"
                     /translation="MEKKRSARSDSAPPIELTGTLALRSRNKTWGNPRRMALLAAIGE
                     EGSITAAAKKVGLSYKAAWDAVDAMNNLADEPLVTRITGGQRGGGATLTPRAVELIAH
                     YEIVSHEHERFMKRLAGVSAGAAHNLELMEHMMIQTSARNKLAGTIRHIKSGAVNDEI
                     IIEIAGGTEIVATITSESVHTLELRVGRRVLALIKASSVLVGLPTEKPAISARNQIPG
                     GISRIVPGAVNGEVSIELAYGQVITAVVSLDSIRALALTEGKPAVAIFMASSVILAGL
                     E"
     gene            7648..8625
                     /locus_tag="VFR20_12560"
     CDS             7648..8625
                     /locus_tag="VFR20_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0231394.1"
                     /translation="MNTFSFVSRRIASGVVMICAVIILNFLLLSAAPGDISDVLAVQA
                     GGATAEQTAQIRKDLGLDKSLVSRLVTYVEQVAQGHLGKSLFYNDSVSSLIAQRLPNT
                     LILVLPAMFIAVVLGTLLGVLSAYKRGGPLNFGVTFLSLAGFAAPVFWSGMVLLIVFA
                     YWLPWFPPSGMFNIGGEPEGLDALWQILHHLVLPVISLSMVYLAQYSRQARATMLDVL
                     GSDYVRTARAKGLRERAVVYRHALKNAILPVLTLAGLQFSHALAGAVLVETVFNWPGL
                     GRLAFDAVLNRDYPLMLGILLVSAILVVVANILVDWLYSVIDPRIKLEA"
     gene            8631..9458
                     /locus_tag="VFR20_12565"
     CDS             8631..9458
                     /locus_tag="VFR20_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0231395.1"
                     /translation="MNTGWKLFLANKGAVFGSLLLILIAFAGLFGKFLYPVSAADIVA
                     APMSPPSWSLPLGADYLGRDVLAGLLHGAGTTLVIGLAATLTTVIIGVVLGATSGFFG
                     RRIDNALMRITEFFQVLPPILLAMVLVAVFGPKIWNVILVIGLVMWPPLARLTRGEFL
                     RLKQAEFVRAEYAMGSSNPNIMFRVLLPNASPPLIVAAALNIGTAILFESGLSFLGLS
                     DPDVASWGYMIGSSRDYIFDAWWSVIFPGVAIFLTVLAISLVGDGLNDALNPKLRQR"
     gene            9540..10562
                     /locus_tag="VFR20_12570"
     CDS             9540..10562
                     /locus_tag="VFR20_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0231396.1"
                     /translation="MGKPLLQVKDLCVEFGSRKRPVRVLDHVDLHLNAGEILGVVGES
                     GCGKSMTSLSILRLVPSPPGRITQGTIELEGTDLLRLSETRMQDVRGNDIAMIFQEPM
                     TALNPVFTIGRQITETLIRHQRLSAEQARRKALELVESVYIPEPAKRLDQYPFELSGG
                     MRQRAMIAMALACRPKVLIADEPTTALDVTVQAQIFDLLLELREKHGTAIILITHNMG
                     AIAELADRVMVMYAGRKIEEGSVGEVLLEPRHPYTRGLLDCVLQVEHDPSKFSPEALP
                     EIPGVVPPLDQLGKGCAFAPRCARVMDKCRTEHPPMLGEPDRGAACWLLDEAPAASAA
                     DKEVLV"
     gene            10559..11584
                     /locus_tag="VFR20_12575"
     CDS             10559..11584
                     /locus_tag="VFR20_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEU0231397.1"
                     /translation="MTENTPLLEVRDLKVHFSIRGKGWLTPSPKVRAVDGVSFSVPRG
                     SAYGIVGESGCGKSTTALALAQLLAPSGGEILLNGESLAHVPPRAMKKLRQRLQMIFQ
                     DPYSSLDPRQRVKDIIAKPMRQLTSMTKPEIDARVNELLGKVGLREEQSRLFPHQFSG
                     GQRQRIGIARALSVRPELVICDEPVSALDVAIQAQVINLLKQLQQDLGLTYLFISHDL
                     AVVQHLCSALSVMYLGRVVEQGGRDQLFSRPLHPYTYALLTADPIKNPQLRGKTQRLR
                     LQGDPPSPINPPAGCHFHTRCPYAIDRCKTEAPPLREVGPSHAVACHRVEIKDGVPTT
                     PNPAFQQ"
     gene            11615..13279
                     /locus_tag="VFR20_12580"
     CDS             11615..13279
                     /locus_tag="VFR20_12580"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU0231398.1"
                     /translation="MARATHITIYKARSIITMNPRQPRATHVAVCDGRILGVGDEASL
                     AGWGPATLDDRFADKVLMPGLIEGHCHLLEGGMWNYVYVGYYDRRGPDGRLWKGLRSI
                     EAVVERLRAAQDDLKDPCAPLYAWGFDPIYFGARRMTAADLDQISSTRRIVVLHASVH
                     LMNVNSAMMAEAGIRRDTAIEGVVLGADGNPTGELLEFPAMYLVFRKIGNIYFDAGQT
                     EQGVWNFGRVAQLAGVTTATDLVNDLSDETLDTLSKVTTDDDYPVRIVPAYSPMRDPE
                     NRALARVLPAVKRNSDKLRFGLVKLVVDGSIQGFTARLRWPGYYAAPEGTAENGIWII
                     PPQQIHDMIKTYHDAGLTVHIHTNGDEATEVTLDAIEAVLRESPRWDHRHTLQHCQMA
                     DAYQFRRMASMGVCANLFANHLYYWGDAHYTMTMGPDRANRMNAAATAKECGVRFTFH
                     SDAPITQLAPLFTAWCAVNRQTASGRVLGESERISVEDALRAMTLGAAYTLKLDDEIG
                     SVEVGKRADFAVLEDDPLQVSPEALKDVRVWGTMLGGRVFACPDHD"
     gene            13272..14282
                     /locus_tag="VFR20_12585"
     CDS             13272..14282
                     /locus_tag="VFR20_12585"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HEU0231399.1"
                     /translation="MTEPRLPLTILGGFLGSGKTTLLNHLLNNREGLRVLVMINDFGS
                     VNIDAALIESGSAAEGVVNLKNGCICCAMASDLMRALLEAEQKADSLDWMVIEGSGVS
                     DPGKIAQIGRAGGTFHLTSIVTVVDGASVRDMVVDRYVGDMVQRQIGAADLILLNKCD
                     LVPARQQADTRAWLRELAPKAAIVSTTHAQVDWDLLRGPIERTPREAEHPPTPAFWTG
                     SKAVGRTASVDFRSIAIHQDAPYDEQKLRRVLEHTDTTVYRIKGVVLVGGDATPSLLQ
                     YTPGGRYALSPWHGGGMRATGTLIAIGTPDIEEDAMRRHFHEAQMEKEPSSDHRITRT
                     TH"
     gene            14296..15906
                     /locus_tag="VFR20_12590"
     CDS             14296..15906
                     /locus_tag="VFR20_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0231400.1"
                     /translation="MLKFAPHRLARPFALGLLSAALLYSSAQAAQLAPVKDGGTLTVL
                     SNSAINSLNPALQSGVATAVPASQLFASLLMADDQWHFHPYLADSWKKSDDGLTYTFK
                     LNPKALFHDGTPITSKDVAFSIMTVKQYHPFGKSMYTNLDKVETPDPHTVVVKFTKPN
                     PAFLLEVSTPALLPILPEHVYGKGNIRQNPHNNAPIGSGPFKFASYKPGQYLTLEKFK
                     RFFLGAPHLDKITYVIIKDDNASTLAIQRGEIQYVAYPPFRLSEVERLSKNPKLVLTH
                     KGYEGVGALVWMEFNMVEKGPLQNEKVRQAISYSIDRNFITQKLQRGQTTPAYGPIHY
                     SSPYYDKNLNHYDVDLARANKLLDEAGYPRKADGTRFELRLDYIPTNPDVSQNVAEYL
                     RPQLKKIGIKINLRPSPDFPTWAKRIANYDYDLDIDPVFNWGDPVIGVARTYMSTNVR
                     KGVVWANMSGYKNPKVDDLLEKAAIENDPAKRKALYDEFQQIVNKELPVTWIATYPTN
                     TIYSKNLRNLPNSIWGPFEPFNNVGWAK"
     gene            15965..17485
                     /locus_tag="VFR20_12595"
     CDS             15965..17485
                     /locus_tag="VFR20_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEU0231401.1"
                     /translation="MKIVIALVRHETNTFSPIQTPLSSFNRGTGDGPVYGEDAIRACR
                     DTNSAAAAYLDMAGRLGAEVDFAIVANAVPSGIVTRDAFDTITQSIVEAVKKGCDAVM
                     LDLHGAMVAEGYPDAEGELLRRLRVVVPASIPIAVALDFHANFSAEMIRNATIITGYC
                     TYPHIDVYQTGERAARTLQAVRDQGVDPVILWRRLPMLTHMLKQTPSMQPMKDIMDHA
                     MAAEAQGRVLNASVFGGFPLADIPHVGLSVVIVADSRKVDEGQALLEELCAMAWERRE
                     DFIFPIEPPAQSIARAKALQEGPVVLVDHGDNCGAGGPTDVMAVLREAMRQGLTDMVA
                     GPFWDPAAVAELVDAGEGSQVTIRIGGKTDMPALGMHGEPLEITGKIVRITDGNYTVT
                     GPMFTGMRLSTGRTAVIDTNGVLVFVCERPQEPYDTGVFTHAGVDPSKKKYVLIKSRQ
                     HFRAGFGPLARHVILVAGPGVCGSDYSQFPFKHLPRPIYPLDKDAQVEHGEFVMAE"
     gene            complement(17548..18261)
                     /locus_tag="VFR20_12600"
     CDS             complement(17548..18261)
                     /locus_tag="VFR20_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0231402.1"
                     /translation="MTVRVQIRRAIRAGEREFVLDVAFESSHRRIALFGPSGSGKTLT
                     VQAIAGLMRPDNGCIVLNDRTLFDSSSGICLAPQARRVAYLFQDYGLFPHLTVAQNVC
                     FGLAKGWRNPRRDVLPEAACHWMEAFELGAVAHHYPHQISGGQKQRAALARALAVQPE
                     ALLLDEPFAALDAALRRKLRAEMAALQRRLDIPMILITHDPEDVRNLAEEVYMIREGR
                     IVGSAAPHEMVAADFSEHH"
     gene            complement(18258..18944)
                     /gene="modB"
                     /locus_tag="VFR20_12605"
     CDS             complement(18258..18944)
                     /gene="modB"
                     /locus_tag="VFR20_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEU0231403.1"
                     /translation="MPDFTVPLFLSLKVAGTAAVFATLLGAGAAFALSRWRSRWCEVV
                     DSFLTLPLVLPPTVMGYYLLVLLGRRGWLGHWLVDWGIELVFTWQGAVVAATVVAFPL
                     VLKSARAAFQNVDEQLENAARVLGIPEAAIFFRVTLPLASRGIFAGVLLAFARALGEF
                     GATLMVAGNIPGRTQTLSIAIYQAVQAGDDNLANILVLITSATCIAALLIASYVWPDH
                     SRRARARERL"
     gene            complement(19086..19841)
                     /gene="modA"
                     /locus_tag="VFR20_12610"
     CDS             complement(19086..19841)
                     /gene="modA"
                     /locus_tag="VFR20_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0231404.1"
                     /translation="MIFKNVAVTIMAGLLAWAPLAHAGDVLVSAAASLTNAFTDLGKQ
                     FQKAHPGTTVHFSFGASDVVLHQIVNGAPADVFASADEKAMDKAAKAKVIDPASRVDF
                     ARNQVVMIVPAGDSLGLKSVADLGQADVKRITLGNPASVPVGRYTRDALEKAGEWKIA
                     QSKAVLANNVRQALDYVARGEVDAGFVFATDAAIMPDKVKVVQALRSPVPVRYPIALV
                     ARKGRSPEARSFVRYVLSTEGQKILAKYGFKRP"
     gene            20269..>21122
                     /gene="xerD"
                     /locus_tag="VFR20_12615"
     CDS             20269..>21122
                     /gene="xerD"
                     /locus_tag="VFR20_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU0231405.1"
                     /translation="MAFSPPPSLDTFLDALWLEDGLAANTLSAYRRDLTAFAQWLATE
                     AGKDLDQAQAADIQDWFAARHATSKATTANRRLAALKRYYLWALRQGRVAADPCLSLH
                     AAKQMARFPKTLSESQVEALLAAPDTDTALGLRDRAMLETLYATGLRVSELVGLGMLD
                     VNLDEGVVRVIQGKGGKDRLVPMGAEAAWWIDSYQKQARPELLGPRRCDALFVTGRAA
                     AMTRQSFWFIVKKYALVADIHVPLSPHVVRHAFATHLLNHGADLRVVQMLLGHADIST
                     TQIYTHVAR"
BASE COUNT         4088 a         7114 c         6404 g         3516 t
ORIGIN      
        1 ataagggtgt caaacgagcg gggtcgccac ttgcgtgcca gtaccagata agtcatgcgg
       61 gagggttacc ctgaggctat cggaagacgg gaaacaaaaa gtgtaccgga ttcaccgacg
      121 ctttggcggg cgattgaaaa acgcctgaaa ggaaggaggc gagcctgact tcggcactgg
      181 ctctgaacga ctatggctgc ttcgttcccg acctgaccag gttcaccgcc gaaccatgcg
      241 aaggggcccg ccaaatgaaa gtttaacagg ttccgacccc taacaatcga tccagagatc
      301 ggcgcgcgga aaatacaggg cggcacgtac cggacggcca tccagggccg ccacggcttg
      361 acggtagcgc tccatctggc cggcatagcg ctcgcgcatg cgggcggcga atgcctcggc
      421 ggactcttct tgcccgggca tgccggtctt gtagtccacg accagccagc cttcctcgct
      481 ggaaatcgcc aggtcgatga ccgacacccg ccccgcggtg tcgaccagcg accactctct
      541 gtgacctcgc gcgacggaca gcagccaccg gccgcgcccg ctcgccaggg tcgcggcgag
      601 ggttgcgcgg accgcgtcgg cggccgcggc cagattggct tcccgcaccc ccgcgcgcga
      661 caactgcgtt ctgaacgccg gcactgcttg ctccacgttg tcggccgtcc actgcgcgcc
      721 accaccgtgc ccacccaggt attccagcca ggcatgcgcc accgtaccaa tggcggcctc
      781 gtcgccgggc tcggcgcgcc actcccagtg tttgccgggc cgcgcagagg cggcggtgac
      841 gggctctgga agatccgcca gcgccaggcg cagcagatct cgatgcctgt cggccggtgt
      901 gccgccggcg tccaatgacg ggaaggtcgg ctcgccacaa tccagatggt cccacaggcg
      961 tcccaatagg ctgccggacg tgggcggacg cgccgcgccg tcctcgaccc gcacgagccc
     1021 gaccagatgc agacgccggc gggcacgcgt cgccgcgaca tacagcaatc tgtcggactc
     1081 gtagtcggcc cgttgtttct cgcgccgcgc gagatagtcc gagaccggat cggatccgtc
     1141 ctgggcgcgg ggcttgatcg ggccgagcaa tatgcggtcg ccatcgagct cgaagcgcac
     1201 caggggtgat cggtcggtcg cgggccggcg gtgcaaaccc atcagcacga cctcatcgaa
     1261 ttccatcccc ttggatttgt gaatggtcat gacttcgaca gccgcgccgt cgccacccga
     1321 cgacgcatag agcccgtcga gacgcgcatc gagctccgtc acgtccgggt ttccataagg
     1381 cgccagcgtt tcgatcaacc gaaagaaacg ttcggcatcc gattggtcct cgacgctggc
     1441 atagacctcg gacccaccca ggcgctgcca gcattgctcc aaccaggccg cgaacggcac
     1501 agcgccggaa cgattgcccg cgtcgagcag gatggtggcc gcgcggcgta gccgccgcgc
     1561 ttcatccgtt gacagaatcg acgtggggac gccgccgcct tccagccagc gcgccaacag
     1621 gaacggcaca gcggaaacat gatccgaacc gaacaacgca tgcagactgg acagggtcat
     1681 cccacacacc ggcgaacgca gcacggaaag ccaggccagg cgatcaccat cgtgcgacag
     1741 cgcgcgcgcc aattgcacca ggtcctcgat cacttggcgc gatttcaacg cctcgagctc
     1801 gactgctcga caagggacgc cttcctgtct gagcaatcgc agcacgcctt ccaggtgatt
     1861 tcttgcgcgc accaggatcg ccaccggatg cgcgctttca gcatcccgat tcgccagggc
     1921 gtccctgacc agaccgagga ccagggactc cgtctgtcgc gtgtgggaat cggcacggga
     1981 atcggtatcc ggcccgtcct cttcgcccga ttgccagacc ggatgaaaac gaaccgccag
     2041 accgtcaccg gcctcgtgaa aggcgcgcga cccggtatac gagatcgcac cgagcgcggc
     2101 atcgtcagcc gggggaaaca atggcttgaa cgtgcgattg acccagtcga cgatgccctg
     2161 atcggaccgg aaattgtcgg tcagccgcag gaactccggc gtgacattcg ttagcccaga
     2221 atcacgaact ttcaggaaaa gccccacctc ggccttgcgg aaacggtaga tggactgcat
     2281 agggtcgccg accaggaaca gcgtgcggcc gtcaccaggc gaccaacccg ccgtcagact
     2341 ctcgagcaac acgatctgcg aatgactggt gtcctggaac tcgtcgacaa ggatatgctt
     2401 gactgacgcg tcgagcttca acagcaactc gctggggtcg tccgcgccac caagggcttg
     2461 caccgcgcgt tgcgcgattt ctatgaaatc gacttcgccc gtttccgcga aacgcaattg
     2521 cagttgcgcc gaagccagac gcaacacccc gagcatcgcg ttcagcgtag ccagctgttc
     2581 gtcggtgtaa ccggaaaaag gcgcgcaccg cgcggcttgc agcgagggcg cccaggcgcc
     2641 ggcatcaggc cgcgcgcgcg ccagccattc ctcgaaggct tgcttgtgcg ggctgcccgg
     2701 cgcgaaaccc tggctcttat tgactttggc ccgtagctca cctttcgcgg tgagcagcgc
     2761 atcggccaac gcccgccacc gtgggccgtc gatcgatgga tcggtgccga acggtgtgcc
     2821 gtcccagtct tccaatgggc caagatcgag cttgccaccc tgatccgcaa gatgccgggc
     2881 cgcggcggcc acactggcgc cgacgtcgcc cgcccatccc attggcatcg cgtcgctcag
     2941 gcgtcgtaga tcctcttcct gggcaagctc caggtttgcg agcagcaggg ccgcgtcatc
     3001 acccgcgtcg agcagcggca gccattgatc ccggcgcgcc aatcccagcg ccaacaattc
     3061 ctgcgccaca cggccatcga catccagatg gtccagcagc gccgccacca ccggctcatc
     3121 gtcgaccatg gccagcgtgg cttgcgctgc cgccaggtag tgcgcacccg agcgatcgga
     3181 cacctttggc aatccgccca ggtggctgag ccagggcatg gcgcgcacca gatgcgcgca
     3241 cagcgaatca atcgtgcgga tggacaggcg cgcggggtac tgcagcaggt tccatttttt
     3301 tgtgtcgtcg tgttccagcg cccttcgggc caaggccagg ctgcgacgct gatgcgccgg
     3361 aatgttcctc ccggcctcgg catccccacg tttctccgcg gcgacaacgg ctcgggcggc
     3421 gtgcagtttc ttcaacaccc gctcatgcat ctcggacgcc gctttgcgcg tgaacgtgat
     3481 ggccacgatt tcctcggggc gctcgacggt ggccagcaag gccagtatgc ggtcggtcag
     3541 cagctccgtc ttgccggagc cagccggagc ctggacgaga aacgaacgcg aaggatccac
     3601 tgcggcgatc cgcacttcaa gatcggacgg gcgcttgtcg atactcacgt tatcggtcct
     3661 cattcaggcg caggaacggc cgcgcatcgc aataatcgag atcggcggaa ttccggattt
     3721 cgttgacggc cctgccctcg gcgtattcgt ctgccaagcc ttcgatcgcg cgccgccagt
     3781 cgtccatgag ccgttcccat gaatgatccg catacagtgg ccagtccgcc ggactggcca
     3841 atccaggcag tccgcaatcg gtatcggcca gcccgcgagc ctcgaccttg cgcgcgtgca
     3901 gcctggcgag caccaatgcc gccacctgcc cggtccgctc agccagcacc gcggcataga
     3961 acggcagttg caagccaatc ggacgttcgc gcatccaatc actcttcggg tcgagccggc
     4021 ccagcccgga tttgtagtcg atgatcgcca tgcgcccgtc ggcaagccga tcgacgcgat
     4081 cgatacgcaa cgataggtcc agaggaccga acgtccaggc gcagtcctgt tcgagcgccg
     4141 ccacggtgaa gggcggccgt tcacattcca gcgcgagcca ggcctccagc accgttcgcc
     4201 cgcgcaggac ttccagcgcc ttgagcgccg gaccgtagtc gcgcagacac tcatcgccgg
     4261 cctgggccgt cgcttgctcg accagatgat ccagcatggc atcgtcccgg gcgcgaagca
     4321 gaccttcctg gttgtccagg agacgccata cgagctcgat ggccttatgc aggaagatgc
     4381 cgcgcgcgtt ccggtcgtgg agactggcat atcccggcag atcggacgcc cccagacggt
     4441 acttcacgaa agcccagagt ggattgcgcg cctgcgtgtc gatgacgccg atgccgcccc
     4501 gggtcttgtc gccgggaacc agcgcgggtc cgttgtcgtc gaccaggcga tcgagcacac
     4561 aaccgcgcac tgggcgccgg gcgacctggg tgacgacaag cttcgcgctg ggcgacgcga
     4621 tcggcgcacc ttccggatgc gttggcgatg cctcgaaaaa cggactggcc cgcaaatcgc
     4681 gctcgccgtc ccgtcgagcg tggctgaacc acacctcggg cgcgctggcc agaaacgacg
     4741 cgaccattgt ccgcgcccat tccaattcgc gatcgggcgt ggcccgtggc gcctgcgccc
     4801 ttcgcagcgc ccccagcgga atgagcggat tggggccagg cacggccggc agaacgtcat
     4861 ccgtcagacc cagaacccag acaccatccc aacggccgcc ttccgattcc agaaagccga
     4921 gcacctcgag ccgcgaggac ggatcgcgct gcggttggaa caaggtctcg tccagtagcc
     4981 gccgcaacaa cgtcagcgcg gccgaggccg tcaaacaacc caacacggtt tcttgcctgg
     5041 ccagattggc cagagcggac tcgaacgcct cgaccacctg atacgcatga ctgtccagcg
     5101 cggcatcgcc gggaaaacca atgcggcgca accattgccg gaataaatcg acccaggcgc
     5161 ccacggtggt ttccgttggc gcaccccgac gagtcgactt ccagtctgtc caggatgcgg
     5221 cgagccgtgg cgccgtgacc gtcagccttt cgacaaactc cttcacagtg acaaccatcg
     5281 tcccgcgtcg tcgccatgcg acgtccagcg cggcgcgggc cgaactctcg gtggacgccc
     5341 cggcgcagca ccccgccaac aaagccgccc ccagcgtatc gacgctgcac gcatcttgct
     5401 cggaaaaaga tgacaggaca cgcagccacg cgagggccgc ccgggcatgg acccattcac
     5461 caagcggcct ggccacggca acgtcataag gccgaacagg ccggagcgac gcgttcaacg
     5521 catcgacggc gtgatcgatg ccaggctcca ggcgtggagt atccgccggc accctcaggg
     5581 cgtcgcgcaa gacgcggtgc gctagaacga catccgaatc caggcgcgcc gcgatgatcg
     5641 cgaaccgccc cgccggccgg cgttcaaggc tgtccgccgc ccactgcgca gccagacgcc
     5701 attcggcgtc gggctcgtcg gccaccacgc gctcgacgcg acatgccggc ccgacgtctg
     5761 ggtggagagc gcatatgcgc acccccgcct cgcgcaacgc cgccatcaga cgcgtctggc
     5821 gtggggacaa cgctgcgaag ccggccagaa ccagtgtctg gaaggacagc gccaaggcac
     5881 cactctcggc cgcgccgcag acgcgctcat actcgagatt gtcgtcctcg gcatccagat
     5941 cagccaacgc cgcgcggtaa tgccggcgcc agacctggaa gcggcgatac tcgtcggttt
     6001 cctcgccagg caacgtttcc gcgtagccgt cggacacgag gcggtcggcg tcgagcgcgt
     6061 gacgcgccgc ctgcgcgatg tcgagaagca cgcgatccgc ctcgtcgcgg gcgatgaccc
     6121 gctcccagag acgccgggag gcgaacgtat cgagccggcg cgcgggcgcc acaccgcccg
     6181 gcgcaaaaca cgcttggccc cccagcgccc gcaaccaggc cgtgaacggc agaatcccag
     6241 gcagtggcgc cgccgcgcgc cccgcaccga gacgcggcgc cagaacggtg accagacgcc
     6301 gcgcgtaccg attgttcacc gtcacgatca ggatgtcgtc ggcattttgc cccactcgca
     6361 gcacgtcctc gacgagctcg tcgaaggaaa tcgaagcgtc cggatcaaga atcatgctgg
     6421 cgacaaataa aacgaaggag attgctacgg tatagcatgc cgacgccgcc ccgcaacccc
     6481 cgaaacaccg gggatccggc atacacttac agaccaacct ggatcaccgc cccaaggaat
     6541 gccgcatgga aaaaaaacgc tccgcccgaa gcgactccgc gccgcccatc gagctgaccg
     6601 gaacgctggc actgcgctca cggaacaaga cctggggcaa tccacggcgc atggccttgc
     6661 tcgccgccat cggggaagag gggtccatca ccgccgcggc caagaaagtc gggctgagtt
     6721 acaaggcagc ctgggatgcg gtcgacgcca tgaacaacct ggccgacgaa cccctggtca
     6781 cccgcatcac cggggggcag cggggcggcg gtgccaccct gaccccgcgc gccgttgaac
     6841 tgatcgcaca ctacgagatc gtcagccacg agcacgagcg cttcatgaaa cgtctggccg
     6901 gcgtcagcgc cggcgcggcg cataacctcg aactcatgga gcacatgatg atccagacat
     6961 ccgcccgcaa caaactggcc ggcaccatcc gccacatcaa atccggggcg gtcaatgacg
     7021 agatcatcat tgaaatcgcc ggcggcaccg aaatcgtggc caccatcacc agcgaaagcg
     7081 tgcacacact ggaattgcgt gtaggcaggc gcgtgctggc gctcatcaag gcgtcctcgg
     7141 tgctggtcgg cctgccaacc gagaaacccg ccatctcggc gcgcaaccag atcccggggg
     7201 gaatcagccg aatcgttccc ggcgcggtca atggcgaagt cagcatagag ctcgcctatg
     7261 gccaggtcat caccgccgtg gtcagcctcg acagcatccg cgcgctggcc ctgacggaag
     7321 gcaagcccgc cgtggccatc ttcatggcct ccagtgtgat tctggcgggg ctggagtagc
     7381 ttcacacgga cgcttttcgc cgtactcgcc tggccacgcg ttggcctttc ggaccaatca
     7441 gtttcagtta tcaaacgata atataaaaaa tcgaaacaga tagttccgtt ttttagggtt
     7501 aacgtgaatt gtcgcctcac ccgataccgt tcaatatgaa cggatcgttc ctatcctagt
     7561 tggcgcatcc gtccgcttcg gaccgcgctc gttctctcga cgcgcagccg ccttccccaa
     7621 tcccgtcttc aagagcggaa tgcctccatg aacacgttca gcttcgtatc gcgcagaatc
     7681 gccagtggcg tggtcatgat ctgcgccgtc atcatcctga atttcctgct gctgtcggcg
     7741 gcgccagggg acatctcgga cgtgctggcc gtgcaggccg gcggcgccac cgcggaacag
     7801 accgcgcaga tccgcaaaga cctggggctg gacaagagcc tggtttcccg tctggtcacc
     7861 tatgtggagc aggtcgccca gggccacctg gggaaatcgc tgttctacaa cgactcggtg
     7921 agttccctga tcgcccagcg gctccccaac acactgatcc tggtcctgcc cgccatgttc
     7981 atcgcggtgg tgctgggcac gctgctgggc gtgctgtcgg cctacaagcg aggggggccg
     8041 ctgaatttcg gcgtcacgtt cctgtccctg gccggcttcg cggcgccggt gttctggagc
     8101 ggcatggtgt tgctgatcgt gttcgcctac tggctgccat ggttcccccc ctcgggcatg
     8161 ttcaacatag gcggggaacc cgaagggctc gacgcgctct ggcagatcct gcatcacctc
     8221 gtgctgccgg tgatctccct cagcatggtg tacctggccc aatacagccg ccaggcccgc
     8281 gccaccatgc tggatgtcct gggttccgac tacgtgcgca cggcccgggc caaggggctg
     8341 cgggaacggg ccgtcgtcta ccgtcacgcg ctcaagaacg ccatcctccc ggttctgacg
     8401 ctcgccggcc tgcagttttc ccacgccctg gccggcgccg tgctggtgga gaccgtcttc
     8461 aactggccgg gcctggggcg gctggctttc gacgcggtgc tcaatcgcga ctaccccctg
     8521 atgctcggca tcctgctggt ctcggccatt ctcgtggtcg tggccaacat cctggtggac
     8581 tggctctaca gcgtgatcga tccacgcatc aaactggagg cgtgacgatc atgaacacgg
     8641 gatggaagtt attcctggcg aacaaagggg ccgtcttcgg gagcctgctg ctcatactga
     8701 tcgcgttcgc gggcctcttc ggaaaattcc tctacccggt cagcgcggcc gacatcgtgg
     8761 ccgcgcccat gtcaccaccg tcgtggtcgc tgccgctggg cgccgactat ctggggcgcg
     8821 acgtgctggc cgggctgctg cacggggcgg gcaccacgct ggtgattggc ctggccgcca
     8881 cgctgaccac cgtcatcata ggtgtcgtcc tcggcgccac atccggtttt ttcggccggc
     8941 gcatcgacaa cgcgctgatg cgcatcacgg agttcttcca ggtgctcccc cccattctcc
     9001 tggccatggt gctggtcgct gtcttcggcc ccaagatctg gaacgtgatc ctggtgatcg
     9061 gcctggtcat gtggcccccg ttggcgcgcc tgacccgagg cgagttcctg cggttgaaac
     9121 aggccgaatt cgtgcgggcc gaatacgcca tgggctcgag caacccgaac atcatgttcc
     9181 gggtgctgct gcccaatgcg tcgccgccgc tcatcgtcgc cgcggcattg aacatcggca
     9241 cggccatcct gttcgaatcg ggcctgtcgt tcctggggct ctcggacccg gatgtcgcca
     9301 gttggggcta catgatcggc tcatcgcgcg actacatctt cgacgcctgg tggtcggtga
     9361 tcttccccgg cgtggcgatt ttcctgacgg tgctcgccat cagcctggtg ggcgacggcc
     9421 tgaacgacgc gctcaacccc aaacttcgcc aacgataggg cgtcatccgc cccacgaaca
     9481 ccatacatgc cggtcgaggc gggtgcccgc caccacccga tcggctcagg gagaaaacaa
     9541 tgggcaaacc cttgctgcaa gtgaaggacc tttgcgtgga gttcggttcg cgcaagcgcc
     9601 ccgtgcgcgt gctcgaccat gtcgatctgc acctgaacgc cggcgagata ctcggtgtgg
     9661 tgggtgaatc gggctgcggc aagagcatga cgagcctgtc catcctgcgc ctggtgccgt
     9721 cgccgcccgg gcgcatcacc caaggcacca tcgaactgga aggcaccgac ctcctgcgac
     9781 tgagcgaaac ccggatgcag gatgtgcgcg gcaacgacat cgccatgatc ttccaggagc
     9841 ccatgaccgc gctcaacccg gttttcacca taggccgaca gatcacggaa accctgatcc
     9901 gccaccagag actcagcgcc gagcaggctc ggcgcaaggc cctcgaactg gttgaatccg
     9961 tgtacatccc cgagcccgcg aaacgcctgg accaatatcc tttcgaattg tcgggcggca
    10021 tgcgccagcg ggccatgatc gccatggcgc tcgcgtgccg gcccaaggtg ctgatcgccg
    10081 acgaacccac cacggcgctg gacgtcaccg tgcaggcgca gatcttcgac ctgctgctcg
    10141 aactgcgcga aaagcacggc acggccatca tcctgatcac ccacaacatg ggggccatcg
    10201 cggagctcgc cgaccgtgtc atggtgatgt acgcgggacg caagatcgag gagggctcgg
    10261 tgggcgaggt actgctcgag cccaggcacc cctatacccg cggactgctc gattgcgtgc
    10321 tgcaagtcga gcacgacccg tcgaagttct ccccggaggc tctgcccgag attcccggcg
    10381 tggtgccgcc cctggatcaa ctgggcaagg gttgcgcgtt cgcgccgcgc tgcgcccggg
    10441 tcatggacaa gtgccgtacc gagcaccccc ccatgctcgg cgaaccggat cgcggcgccg
    10501 cctgctggct gctcgatgag gcccccgccg cgagcgcggc tgacaaagag gtgctggtat
    10561 gaccgagaac acccccttgc ttgaagtccg cgacttgaag gtgcattttt ccatccgggg
    10621 caagggatgg ctcaccccgt ccccgaaagt gcgggcggtc gacggcgtct cgttttccgt
    10681 gccgcgcggc agcgcctacg gcatcgtggg cgaatccggt tgcgggaagt ccaccaccgc
    10741 cctggcgctc gcccagctgc tggcgcccag tggcggggag atcctgctca acggcgaatc
    10801 gctcgcccat gtgccaccca gggccatgaa aaagcttcgc cagcgcctgc aaatgatttt
    10861 ccaggaccct tattcgtccc tggatccgcg ccagcgcgtc aaggacatca tcgccaagcc
    10921 gatgcgtcag ctgacctcca tgaccaagcc cgagatcgac gcccgcgtca acgagcttct
    10981 gggcaaggtg gggctgcgcg aggagcagtc ccggctgttc ccgcaccagt tctcgggggg
    11041 ccagcggcaa cgcatcggga tcgcccgcgc gctgtccgtg cgcccggagc tggtcatctg
    11101 cgatgagccc gtgtcggccc tggacgtcgc catccaggca caagtcatca atctgctcaa
    11161 gcaactgcag caagacctgg gcctgaccta cctgttcatt tcgcacgacc tggccgtggt
    11221 gcagcacctg tgcagcgcgc tcagcgtcat gtacctgggc cgcgtcgtgg agcaaggcgg
    11281 ccgcgaccag ttgttcagcc gcccgctgca cccctacacc tacgccttgc tgacggcgga
    11341 tccgatcaag aatccgcaac tgcggggcaa gacacagcgt ctgcgcctgc agggggaccc
    11401 gcccagcccg atcaacccac ctgccggatg tcatttccac acgcgctgcc cgtacgcgat
    11461 cgaccggtgc aagaccgagg cgccgccact ccgggaggtc ggaccaagcc acgccgtggc
    11521 ctgccacagg gtggagatca aggacggcgt gcccaccacg ccaaaccccg ccttccagca
    11581 atagccgcca tccatactca tcaagggaag caccatggcc cgcgccacgc acatcaccat
    11641 ttacaaggcc cgcagcatca tcaccatgaa cccccggcaa ccgcgcgcca cccacgtcgc
    11701 ggtgtgtgac gggcgcatcc tgggcgtggg cgacgaggcg tcgttggcgg gctgggggcc
    11761 ggccacactg gacgatcgct tcgccgacaa agtcctcatg cccggcctca tcgaggggca
    11821 ctgccatctg ctcgagggcg ggatgtggaa ctatgtgtac gtgggttact acgatcgccg
    11881 gggtccggac ggcaggctgt ggaaggggtt gcgcagcatc gaggccgtcg tggaacggct
    11941 gcgcgcggcc caggacgacc tgaaggaccc ctgcgcaccg ctgtatgcct ggggtttcga
    12001 cccgatctac ttcggtgcgc gcagaatgac cgccgccgat ctggatcaga tctccagcac
    12061 ccggcgcatt gtcgtgctcc atgccagcgt gcacctcatg aacgtcaatt ccgccatgat
    12121 ggctgaagcc ggcatccgtc gcgacacggc catcgaaggc gtggtgctcg gagccgacgg
    12181 gaacccgacc ggcgaactgc tcgaattccc cgcgatgtat ctggtgttcc ggaaaatcgg
    12241 caatatctac ttcgacgcgg ggcagacgga gcaaggcgtc tggaacttcg gacgggtggc
    12301 tcaactggcg ggggtgacca ccgccacgga cctcgtcaat gacctgagcg acgaaacgct
    12361 tgacacgttg agcaaggtca cgaccgacga cgactatccc gtacgcatcg taccggccta
    12421 ctcgcccatg cgggacccgg agaaccgggc gctcgcgcgt gtgttgccgg ctgtgaagcg
    12481 caattccgac aaacttcgat tcggcttggt gaaactggtg gtggacggct ccatccaggg
    12541 gttcaccgcg cgcctgcgct ggcccggcta ttacgccgcg cccgagggca ccgccgagaa
    12601 cggcatatgg atcattcccc cgcagcagat ccatgacatg atcaagacct accacgacgc
    12661 ggggctcacg gtgcacatcc acaccaatgg ggatgaggcg accgaggtca cgctcgacgc
    12721 catcgaggcg gtcctgcggg aaagcccgcg ctgggaccat cgccacaccc tgcagcactg
    12781 ccagatggcc gatgcgtacc agttccggcg catggccagc atgggggtat gcgcgaatct
    12841 gttcgccaac cacctctact actggggcga cgcgcactac accatgacca tggggccgga
    12901 tcgcgccaac cgcatgaacg ccgccgccac ggccaaggaa tgcggcgtgc gcttcacatt
    12961 tcactcggac gcgccgatca cacaactggc accgttgttc acggcctggt gcgcggtcaa
    13021 ccgccagacc gccagcgggc gcgtcctggg cgagagcgag cgcatctccg tcgaggatgc
    13081 cttgcgcgcc atgacactgg gagcggccta taccctcaag ctcgacgacg agattggtag
    13141 cgtggaggtc ggcaagcgcg cggacttcgc ggtgctcgag gatgatccgt tgcaagtgtc
    13201 ccccgaagcg ctcaaggacg tgcgggtctg gggcaccatg ctggggggcc gcgtgttcgc
    13261 gtgcccggat catgactgaa ccccgtcttc cgcttaccat cctgggaggg ttcctgggtt
    13321 cgggcaagac caccttgctc aatcacttgc tgaacaaccg cgagggattg cgcgtgctgg
    13381 tgatgatcaa cgatttcggc agcgtcaaca tcgatgccgc cctgatcgaa tccggcagcg
    13441 cggccgaggg ggtcgtcaac ctgaaaaacg gctgcatctg ctgcgccatg gcctcggacc
    13501 tgatgcgcgc actgttggaa gccgagcaaa aagccgacag cctggactgg atggtcattg
    13561 agggcagcgg cgtgagcgat ccaggaaaga tcgcacaaat cggccgcgcg ggcggaacct
    13621 tccacctgac atccattgtc acggtggtgg atggcgcgtc ggtgcgcgac atggtggtgg
    13681 accgatacgt cggcgacatg gtgcagcgcc agatcggcgc ggcggatctg atcctgctga
    13741 acaagtgtga tctcgtgccc gcgcgccagc aggccgatac gcgggcgtgg ctgcgcgaac
    13801 tggcgccgaa ggcggcgatt gtttccacca cccatgcaca agtggattgg gatttgttga
    13861 gggggcccat cgaacgaacg ccgcgggaag cggagcatcc accaaccccg gccttctgga
    13921 ccgggtcgaa ggccgtcggc cggacagcct ccgtcgactt ccgcagcatc gccatacatc
    13981 aggacgcgcc ctacgacgaa caaaagctgc gccgggttct ggagcacacc gacacaacgg
    14041 tttaccggat caagggtgtc gtgctcgtgg gaggggacgc cacacccagc ctgctgcaat
    14101 acacacccgg cggacggtat gcgttgtccc catggcatgg cggcgggatg cgggcaaccg
    14161 gcacattgat cgccattggc actcccgaca tcgaggaaga tgcaatgcgg cgacattttc
    14221 acgaagcaca gatggaaaaa gagccatcca gtgaccaccg catcacgaga acaacccatt
    14281 gaacaggaga acaccatgtt gaaattcgcc ccccatcgat tggcccggcc cttcgcactg
    14341 ggattgctgt cggccgcgtt gctgtactcg tcggcgcagg ccgcgcaact cgcgcccgtc
    14401 aaggacggcg gcacgctgac cgtgctgtcg aacagcgcca tcaactccct gaatcccgcg
    14461 ctgcagtcag gggtcgcgac cgcggtcccg gccagccagc tgttcgccag cctgctcatg
    14521 gccgatgacc agtggcattt ccatccctac ctggccgact cctggaagaa gtccgacgac
    14581 ggcctgacct acaccttcaa gctcaacccc aaggccttgt tccatgacgg cacgcccatc
    14641 acctcgaaag atgtggcgtt ctccatcatg accgtcaagc aataccaccc gtttggcaag
    14701 tccatgtaca ccaacctgga caaggtcgaa acccccgacc cgcataccgt ggtggtgaag
    14761 ttcaccaagc ccaaccccgc cttcctgctg gaagtgtcca ccccggcgct gttgcccatc
    14821 ctgccggaac acgtctacgg caagggcaac atccgccaga acccccataa caacgcgccc
    14881 ataggctccg ggccgttcaa gttcgccagc tacaaacccg gccagtacct gaccctggag
    14941 aagttcaagc gcttcttcct gggcgcgccg cacctggaca agatcaccta cgtgatcatc
    15001 aaggatgaca acgcctccac gctggccata cagcggggcg aaatccagta tgtcgcgtat
    15061 ccgccattcc gcctctccga ggtggagcgc ctgtccaaga accccaagct ggtgttgaca
    15121 cacaaagggt atgaaggcgt gggcgcgctg gtgtggatgg aattcaacat ggtcgagaaa
    15181 ggcccgttgc aaaacgagaa ggtgcgccag gccatctcgt attccatcga ccggaacttc
    15241 atcacccaga aattgcagcg tggccagacc acccccgcct atggcccgat tcactactcc
    15301 agcccgtatt acgacaagaa cctgaatcac tatgacgtcg acctggcccg tgccaacaag
    15361 ctgctcgacg aagccggtta ccccaggaag gccgacggca cacggttcga gctccggctg
    15421 gactacattc ccaccaaccc cgacgtctct cagaacgtgg ctgaatacct gcgcccgcag
    15481 ttgaagaaga tcggcatcaa gatcaatctg cgtccatccc cggacttccc cacctgggcg
    15541 aaacgcatcg ccaactacga ctacgatctg gacatcgacc cggtgttcaa ctggggcgat
    15601 cccgtcatcg gcgtggcgcg cacgtacatg agcaccaacg tgcgcaaggg cgtggtgtgg
    15661 gccaacatga gcggctacaa gaatcccaaa gtggatgacc tactggagaa agcggccatc
    15721 gagaacgacc ccgccaagcg caaggcgctg tatgacgagt tccagcagat tgtgaacaag
    15781 gaactgccgg taacgtggat cgccacctat cccaccaaca cgatctactc caagaacctg
    15841 cgcaacctcc ccaatagcat ctggggcccg tttgagcctt tcaacaacgt cggatgggcg
    15901 aagtaagcct cggcaaacac tcgacgccac tcttacacac tgggaaattc attcggaatc
    15961 atctatgaaa atcgttatcg cgttggtcag gcacgaaacc aacactttct ctcccataca
    16021 gacgccactg tcgtcattca atcgcggcac gggcgatggc ccggtttatg gtgaggacgc
    16081 catccgcgcg tgccgggaca ccaacagtgc cgctgccgca tatctggaca tggcgggccg
    16141 cctgggcgcg gaagtggatt tcgcgatcgt ggccaacgcc gtgcccagtg ggatcgtgac
    16201 acgtgacgca ttcgacacca tcacgcaatc aatcgtcgag gcggtgaaga aaggctgcga
    16261 cgccgtcatg ctggatctgc acggcgccat ggtggctgaa ggataccccg atgccgaggg
    16321 cgaactgctg cggcgactgc gcgtagtcgt gccggcttcg atccccattg ccgtggcgct
    16381 tgatttccat gccaacttca gcgccgagat gattcgtaac gcgaccatca tcaccggcta
    16441 ctgcacctat ccgcatatcg atgtctacca gaccggtgaa cgcgcggcac gaacgttgca
    16501 agccgtacgg gatcagggcg tcgatccggt gattctctgg cgtcgcctgc ccatgctgac
    16561 gcatatgctg aagcagacgc ccagcatgca gcccatgaaa gacatcatgg accacgccat
    16621 ggcggccgaa gcccaaggac gggtactgaa cgcttccgtg ttcggcggct tcccgttggc
    16681 cgacattccg catgtcgggt tgtccgtggt catcgtggcg gacagtcgca aggtggacga
    16741 aggccaggcc ctgctggaag aattgtgcgc catggcctgg gaacgccgcg aagatttcat
    16801 attccccatt gagccaccag cgcaatccat cgcccgggcc aaggccttgc aggagggtcc
    16861 cgtggtgctg gtcgaccacg gggacaactg tggcgcggga gggccgacgg acgtcatggc
    16921 cgtgctgcgg gaagccatgc ggcaaggcct gacggacatg gtcgccggac ctttctggga
    16981 tccggccgcg gtggccgaac tggtggacgc gggtgaaggc agccaggtga cgatccgcat
    17041 tggcggcaaa accgatatgc ccgcgcttgg catgcacggc gaaccgctgg aaataacggg
    17101 aaagatcgtc cgcatcaccg acggcaacta taccgtcacc ggaccgatgt tcaccggcat
    17161 gagactcagc acaggccgca cggcggtcat cgacaccaac ggcgttctgg tttttgtttg
    17221 tgaacgccca caggaacctt acgacacggg ggtcttcacg cacgccggcg tggatccgtc
    17281 caagaagaaa tacgtgctca tcaaatcacg ccagcatttc agggcgggct ttggtccgct
    17341 ggcgcggcac gtgatcctgg tcgcgggtcc gggtgtctgt ggctccgact attcacagtt
    17401 cccgttcaag catttgccac gtcccattta tccgttggat aaggacgcac aggtcgaaca
    17461 cggtgagttc gtgatggctg agtgatccgc gccggttccg agcagtcatc ggaatcggtg
    17521 gcctttccac cggcgcgcac agacgaatca atgatgctcc gaaaagtccg cggcaaccat
    17581 ttcgtgtggc gccgccgatc cgacaatccg tccttcacgg atcatataca cctcttcagc
    17641 cagattgcgc acgtcctcgg ggtcgtgtgt gatcaggatc atgggaatat ccaggcgccg
    17701 ctgcaaggcg gccatctcgg cgcgaagctt gcgtcgcaac gccgcatcca gagcggcgaa
    17761 cggctcgtcc agcagcagcg cctctggctg cacagccaac gctcgagcca gggccgcgcg
    17821 ctgcttctgc cctccggaaa tctgatgagg gtagtgatgc gcgacagcgc cgagctcgaa
    17881 ggcctccatc cagtggcacg cggcttccgg caacacgtca cggcgcggat tgcgccagcc
    17941 ctttgcgagg ccgaaacaca cgttttgcgc caccgtcaga tgaggaaaca acccatagtc
    18001 ctggaacaag taagccactc gcctggcttg cggcgccagg catatgccgg acgatgagtc
    18061 gaataatgtg cgatcgttga gcacgatgca gccattgtcc ggacgcatca gccccgcgat
    18121 ggcctgcacc gtcaatgttt tgcccgaccc tgacgggccg aacaaagcga tacggcgatg
    18181 cgacgactcg aacgccacat ccagaacaaa ctctcgttcg ccagcgcgga tggcgcggcg
    18241 gatttgaaca cgcacggtca caaccgctcc ctggcacggg cacgccgtga atgatcaggc
    18301 cacacatagc tggcaatcag caaggccgcg atgcaggtgg ccgatgtgat caacaccagg
    18361 atgttggcca gattgtcgtc gccggcttga acagcctgat agatggcgat ggacagtgtc
    18421 tgcgtacggc ccgggatatt gcccgccacc atgagggtgg cgccaaactc gcccaacgcc
    18481 cgcgcgaagg ccagcaacac tccggcgaat atgccgcgcg aggccagcgg gagcgttact
    18541 cgaaagaaga tcgccgcctc cgggatgccc agcactcttg ccgcgttctc cagttgttca
    18601 tcgacgttct gaaacgccgc ccgcgccgat ttcagcacca gtggaaacgc cacgaccgtg
    18661 gccgcgacca cggcgccctg ccaggtgaac accagctcga taccccagtc caccagccag
    18721 tgtcccagcc agccacggcg acccagcaaa accagcaggt agtatcccat cacggtgggc
    18781 ggcagcacca atggcaaggt gagaaaggaa tccaccacct cgcaccagcg cgaccgccag
    18841 cgcgacaagg cgaacgccgc ccccgccccc aacaaggtgg cgaagaccgc ggccgtgccg
    18901 gccactttca aggataggaa aagaggaacc gtgaaatcag gcatggcgac ccgtcacccg
    18961 atgacgcgtc gcacactgcc tttccgcgcg tttctgaata gcattctccg gagccgattg
    19021 actgagaatc gatcgacagc gggctggttg accgcgaatc atctcacctc gcatcagact
    19081 actcatcacg ggcgcttgaa tccgtacttg gccagaatct tctgcccttc cgtcgacaac
    19141 acatacctta caaaggaccg agcctcgggg ctgcgcccct tgcgtgccac gagcgcgatg
    19201 gggtagcgca ccggcaccgg cgacctcaac gcctgcacga ccttgacctt gtcaggcatg
    19261 atggccgcgt ccgtggcaaa cacgaaaccg gcgtcgacct cgccgcgcgc cacatagtcc
    19321 agcgcctggc gcacgttatt ggccagtacc gccttggatt gcgcaatctt ccattctccg
    19381 gccttttcca gcgcgtcacg ggtgtagcgg cccacgggca cggatgccgg gttgcccagc
    19441 gtgatgcgct tgacgtcggc ctgaccgaga tcggcaaccg acttcaaccc cagcgaatcg
    19501 ccggccggca cgatcatgac cacctggttc cgcgcgaaat ccacgcgcga cgcgggatcg
    19561 atcaccttgg ccttcgccgc cttgtccatg gctttctcgt cggcggaagc gaacacgtcc
    19621 gccggtgcgc cgttgacgat ctgatgcagc acgacgtccg acgcgccgaa actgaaatgc
    19681 acggtggtgc cgggatgcgc cttctggaac tgttttccca gatccgtgaa ggcgttcgtc
    19741 aggctggcgg ccgccgacac gagaacgtcg ccggcgtgcg ccaggggtgc ccaggccagc
    19801 aggccggcca tgattgtcac ggccacattc ttgaatatca ttcttgtttc ctccgagtct
    19861 cgccgaatga ctgaataacc gcttggctgc agactgtgca caccatcacg gtccgcatcg
    19921 gtcgccggat cgcccactcg gcaaacccag cgacagaagc gccaccatgc ctgattcgca
    19981 tcaggcaaga tcgaaatata tatacgtata taacgatagt caaacccgcg tatcatgggt
    20041 atccaccaat accactgcat tagtcgcgtc cggagccggg acgctctgat gccggtctga
    20101 cccgctggca tacaggcggt cggattgccc ctcccctttg atttgcgacc gcgccgaccc
    20161 cggtctaaga ttcgacaaga agaacatctg acccatccag gcggaaagtc ggcactgcgg
    20221 atcagaaggc gtcccatcca ttctgtcatt tccatttccc ggaccaccgt ggctttttct
    20281 ccccccccct cgctcgacac attcctggac gcgctatggc tggaagacgg tctcgcggcc
    20341 aacacgctgt cagcgtatcg ccgcgacctc accgctttcg cgcagtggct ggccacggaa
    20401 gccggcaagg acctggatca ggctcaggcc gcggacatcc aggactggtt cgcggcgcgc
    20461 cacgccacca gcaaggccac caccgccaac cgtcggctcg ccgcgctgaa gcgatactat
    20521 ctgtgggcgc tgcgacaagg ccgggtcgcg gcggatccat gcctgtcgct tcatgccgcc
    20581 aagcagatgg cccgctttcc caagaccttg tcggaaagcc aggtggaagc tctgctggcc
    20641 gcgcccgaca ccgacaccgc gctgggcctg cgcgaccgcg cgatgctgga gacgctctat
    20701 gcgacgggcc tgcgcgtgag cgaactggtg ggcctgggta tgctggacgt caatctggat
    20761 gaaggggtgg tgcgtgtcat tcagggcaag ggagggaagg accgactggt ccccatgggg
    20821 gccgaggcgg cttggtggat tgacagctac cagaaacagg cacgcccgga actgctcggg
    20881 ccgcgtcggt gcgacgcctt gttcgtcacg ggccgggcgg cggcgatgac ccgccagtcg
    20941 ttctggttca tcgtgaagaa gtacgcgctg gtggcggaca tacacgtgcc gctctcaccc
    21001 cacgtggtgc ggcacgcttt cgccacgcat ctgctcaacc atggcgccga tctgcgggtg
    21061 gtgcagatgt tgctgggcca tgctgatatc tcgaccacgc agatatacac ccatgtcgcc
    21121 cg
//
LOCUS       DASTNK010000173        21125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1280872, whole genome shotgun sequence.
ACCESSION   DASTNK010000173 DASTNK010000000
VERSION     DASTNK010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 21125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21125
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280872"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VFR20_12620"
     CDS             complement(<1..713)
                     /locus_tag="VFR20_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEU0231406.1"
                     /translation="MNDTSKTKSAAGAAILVGALGVVFGDIGTSPLYTIKVSLAQYGT
                     PSHEHILGVLSVLFWLLMLVVSLKYVTLILRADNRGEGGTLALTELAVRGMTRRRGMV
                     LTVLGLIGAALFYGDSMITPAISVLSAVEGIGVVSHEFDELIVPISVAVLVALFLIQS
                     RGTGTVGKLFGPIMLLWFVTLGVLGAWRIWLTPKILLALNPLYAWRLIADGPLETFLL
                     LGAVVLALTGAEALYADMG"
     gene            complement(736..1800)
                     /locus_tag="VFR20_12625"
     CDS             complement(736..1800)
                     /locus_tag="VFR20_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0231407.1"
                     /translation="MLVPEGSLARHLVMRLLPALIALILLDLVVTWVVTRKVGLEAWV
                     LRDIFWTMMTSQVLLVSLFAWVLVSGVRSGLRSINRLSDEISQRSIDDLQPLDIAGLP
                     TEVAPVVTHFNDLLVRLDDSMQAQKRFIGHAAHQLRTPLSGLKLESELMLGRPLPDDV
                     RARAERIKSVTDRMIRLGQQLLVLARADSSTRPQDSFVRTDLCEWARINGAEWIAAAR
                     AHKVDIQLSAPDEPVWIDADPLLLEELLGNLIDNALRYGTGAKVIGLKVTPNPPSLVI
                     EDDGPGIDPADSERVFEAFYRSPKVNKAGSGLGLAIVREIARAHGAWWSLLSRPDFPG
                     TRISVVFPGPRIGARLTRQE"
     gene            complement(1923..2597)
                     /locus_tag="VFR20_12630"
     CDS             complement(1923..2597)
                     /locus_tag="VFR20_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0231408.1"
                     /translation="MRVLVIEDDTTLGHALQEFLVEQGYAVDWLKDGDQVLGAVAGQT
                     YDLLLLDLNLPGMSGLDVLRQLRSGGQQTPVLILTARDGVEDRVAGLDAGADDYVTKP
                     FELPELAARVRAFARRRAGQAQPFLEAGPLMFDTVGREVRVNGERLALSVRELSVLEM
                     LMGRVGRVVTKHQIVNSLSAWDADFSENAVEVYVYRLRKRLDGTGASIQTVRGFGYLL
                     DVESAA"
     gene            complement(2692..3117)
                     /locus_tag="VFR20_12635"
     CDS             complement(2692..3117)
                     /locus_tag="VFR20_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEU0231409.1"
                     /translation="MAFGGFENKGGGQTMSEINMVPLIDVMLVLLVIFIITAPLLTHS
                     IKVDVPRVSAQPTAAEPDAVDLAIDAKGRMFWNDEPVDMSQLGQRLDTMATQNPQPRL
                     RLRADLNTRYEVLARIMGLARAAGVTRLGFVTQPSGASP"
     gene            complement(3119..3952)
                     /locus_tag="VFR20_12640"
     CDS             complement(3119..3952)
                     /locus_tag="VFR20_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEU0231410.1"
                     /translation="MFDVLHSAIQVLSQVGQAAAPAIVAVAAGGGSSTQTSGVIDFVS
                     HSDWVGKSLFGILLLMSLVSWYLIFVKIFMTMRNRRRSTKFLNEFWAASSLEQVENEI
                     AAHGVTEPFSHLTSHAIHARNHHAKYGALKLEETGSTSAFVTRTIRKVLDEDTARLEN
                     GLTILASIGSTAPFVGLFGTVWGVYHALVGIGLSDGVSINRIAGPVGEALIMTGLGLA
                     VAIPAVLAYNGFVRGNRVYLARLDAFAHDLFTFLITGQQVAENSGKARPVALAASGKG
                     H"
     gene            complement(3989..4258)
                     /locus_tag="VFR20_12645"
     CDS             complement(3989..4258)
                     /locus_tag="VFR20_12645"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEU0231411.1"
                     /translation="MTHVAYQGRPPTPRYPDAALRRGQTGKVVIRVVISPQGNVVKAT
                     VERSSGHALLDAAALDAVRRARFRPYTENGVAYSARADIPIDFVL"
     gene            complement(5128..5376)
                     /locus_tag="VFR20_12650"
     CDS             complement(5128..5376)
                     /locus_tag="VFR20_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231412.1"
                     /translation="MKSGFVVVCAAVSLLSGCALHHGLDQASLQEYQKQCDQLGFKSG
                     TDAYSNCLLKQQEMDNDNTQRQMDRFIWQTPNQQRKGG"
     gene            5747..7672
                     /locus_tag="VFR20_12655"
     CDS             5747..7672
                     /locus_tag="VFR20_12655"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014899350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0231413.1"
                     /translation="MTSPGNPYNPFSSHPPTRSSRRRFLGHTGAGMLAAVLPGRAGAN
                     GQLASSYSQTIAYGRQAIQQLMSGNQNISTISVALLKSQQIVWQQAFGMASMTNSIPA
                     TLQTRYNIGSVSKVLAALVATILQDRGLISLETPMVQYLPAFTMLSPEYKRITVRHLL
                     SHTSGFPGTNGRNAFMLGVPATGYAADTEAALANQHLKHLPGELAVYCNDGFTMIEQI
                     VLAVTGQPYADFVQENLLTPLAMTDSGFLTSVPSVGEYAYPWLNGKQYAQEFVSPLAS
                     GGLSTTPTDMMNLARMLIGGGLFRGRRIVSAAGIAEMATDQTTKLLINPSPEWRWGLG
                     WDSVHQLGLAAAGVNAWQKGGNSAFFETQFMVLPDAQMALMVTGNNGFGAEKLAEGIL
                     IKALLEQGALAAAPARVSDMAPPVAPAPNVLDAAGIYGNSSVPRQVIVAADGGFTLNK
                     WNGTTWVPDGSAPYQYRSDGWWWSTGQGPLSFRFEVVANTDEEGKPFSYRYLMMRYVT
                     GAGYNHITLPMGQQLSGPQLPALPALDPVWQARMGIIWNLTNQSPDSLPSQFSGPLQA
                     TLSEPPWLQGYILLHNNDYDLGAPQYQLLVPLANDRGGMAVKVPVIQGRDLQEISFSS
                     VNGVPTMTIGSWIYTAA"
     gene            complement(7750..7825)
                     /locus_tag="VFR20_12660"
     tRNA            complement(7750..7825)
                     /locus_tag="VFR20_12660"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7790..7792),aa:Val,seq:tac)
     gene            complement(7949..8224)
                     /locus_tag="VFR20_12665"
     CDS             complement(7949..8224)
                     /locus_tag="VFR20_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU0231414.1"
                     /translation="MNKTELIEHISSKADLSKADAGRALEAFIGAVKTTLKKKGGSVT
                     LVGFGTFAVSERAARTGRNPRTGEAIKIKKAKVPKFRPGKALKDAVN"
     gene            complement(8597..9832)
                     /locus_tag="VFR20_12670"
     CDS             complement(8597..9832)
                     /locus_tag="VFR20_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HEU0231415.1"
                     /translation="MSKNGFTTTILHSDRRAGAEHGAVHKPMHPSSQYAYADARELAA
                     VFQGKPGYTYARQGTPTTAALEAQVTQMEEGLATISFATGMAALNATFFALLRRGDHL
                     ISSKYIFGNTNSLLGTLAALGVEVTLVDATNIDDVRQAWRPETRMVFTESIANPGTQV
                     ADMRAIGDWCREKNLVYVIDNTLSTPWLSTGKAFGASLVMNSLSKYIGGHANALGGAV
                     TNTGLYDWSTYPNIAEPYRKGPGNTWGLTQIKKKGLRDMGGTLSSEAAHRIAAGAETL
                     ALRMDKCCANSLSLARFLDSHPKVAKVHYPGLASHPQHERAADYFGGRFGALMGVDLV
                     EGIDVFEFLNHLDVLVLATHLGDTRTLVLPMAQTIYYEMGAKQRALMGISDTFLRVSV
                     GIEDTEDLIGDFTQALARF"
     gene            complement(9912..10664)
                     /gene="rlmB"
                     /locus_tag="VFR20_12675"
     CDS             complement(9912..10664)
                     /gene="rlmB"
                     /locus_tag="VFR20_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU0231416.1"
                     /translation="MSSHQVLAGFHAAVARMRHAPASIVDLYVDASRRDKRMLSLLEQ
                     AQAAGVRVHPVPAERLDGLAHGTRHQGVVALAQSQKLAIDVDEVLDGLEDTGIAPFLL
                     ILDGVTDPHNLGACLRTADAAGVHAVIAPRDRSVGLGATVARVACGAAETVPYIMVTN
                     LARTMRHLKDRGLWLIGTDDQADTRFHGVDACRPMAWVLGAEGEGMRRLTRETCDERV
                     AIPMLGSVESLNVSVATAVCLYETVRQRMAAG"
     gene            complement(10760..13201)
                     /gene="rnr"
                     /locus_tag="VFR20_12680"
     CDS             complement(10760..13201)
                     /gene="rnr"
                     /locus_tag="VFR20_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU0231417.1"
                     /translation="MTKRSKNEINNNEILNGLPETPPDFDPDVPSREAILTQLRMAGQ
                     PLSPEALARKVGVSEPFPVGFERRIKAMERDGQLLRNPGGQLLINNKSDFIAGRVQGH
                     RDGFGFLLRDDGGPDLFLSPREMLKVLHGDRVLAKPDGMYRGKPEAVIVEVVERRTNR
                     LVGRFLKEHGATIVAPEDQRIKHDILIPPGEAGGAENGQVVTVEIVQQPTRHTQPLGR
                     VVEVLGEIDDPGMEIEIAVRKFDVPLDFSDAALKQAARLPAKVRPAELPGRVDLRDLP
                     FVTIDGEDARDFDDAVFCLPAESGNGARKTKGWRLLVAIADVSHYVRPGDALDDDAVE
                     RGTSVYFPRRVIPMLPETLSNGLCSLNPDVDRLVMVCDMFIPLGGKKAGTVSAYQFYN
                     AVIHSHARTTYTDVWAALQQPDGPAAQAMAEVLPHLRDLYELYQLLAEARVRRGAIDF
                     DTVETKILCNPLGRIEKIVPYTRNDAHRLIEECMLAANTCAADFMTRNKRMGLYRVHE
                     GPTPEKLQALRDFLRNLGLSLEGGDDPTTHDYARLVQSARNRPDFEVVQTMCLRSMQQ
                     AIYSPDTIGHFGLSYEHYAHFTSPIRRYPDLLTHRVIKSIIDGTRYVPKVDDAAAVAS
                     LPKHDREHAAWEKLGLLLSASERRADEASRDVEAWLKCWFVKERVGEVFSGRVTGVAS
                     FGVFITLDTLFVEGMVHVSELGSDYFQFNEAMHELRGERTGIRYRLTDAVQVQVARVD
                     LEARRIEFRLVKGTSFKALSQPESGTPHRVKRAASAKPDSLKGPSRERRAAAKRNARA
                     SALAVGKKSGHRGRK"
     gene            13307..13391
                     /locus_tag="VFR20_12685"
     tRNA            13307..13391
                     /locus_tag="VFR20_12685"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13341..13343,aa:Leu,seq:cag)
     gene            13649..14461
                     /locus_tag="VFR20_12690"
     CDS             13649..14461
                     /locus_tag="VFR20_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEU0231418.1"
                     /translation="MLASLGKRLAPLLLSIIASGALAQTPKTVFLQDLTWTELRAEIN
                     AGKTTIIIPIGGTEQSGPDIALGKHNARVRVLSQRIAQTLGNALVAPVIAYVPEGSYA
                     PPTAHMRFPGTITIPDDAFEKTLESAANSFKIHGFLNIVFLGDHGGYQNDLKLVVHRL
                     NRSWSGTKARAFVPPAYYGTSSRGFAQILRKRGYHDAEIGTHAGLADTSLLLAVAPSM
                     VRLDRLRSGPTPGAAEGVYGDPRRASAELGQLGVEAIISRTVAAIRKDTAGR"
     gene            14537..15721
                     /locus_tag="VFR20_12695"
     CDS             14537..15721
                     /locus_tag="VFR20_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEU0231419.1"
                     /translation="MYSLCFPARRRAARFVPALGLVLGILCAGPVASAATSIDTVPGM
                     PPVVNPNNLYSEAGVNHFSPAVAGALSRVYVPNVRSNDVYVIDPATFKVVDKFRVGRN
                     PQHIIPSWDLKTLWVANNDEGRRGGSLTPINPKTGKPGPAIPVEDPYNMYFTPDGKYA
                     IVVAEALARLDFRDAHTMALKVSLKVPGCKGINHADFSIDGRYAIFTCEFDGSVAKID
                     MVNRKVVGYLRFAKPSMPQDIRISPDGRIFYVADMMANGVHLIDGDTFKEIGFIKTGV
                     GTHGLYPSRDGTHLYVSNRGSNLVHGPRHGKGSVSVIDFATRKVVANWPIPGGGSPDM
                     GNVSADGKMLWLSGRFDDVVYAINTTTGAVKSIPVGMEPHGLTVWPQPGRYSLGHTGN
                     MR"
     gene            complement(15757..16236)
                     /locus_tag="VFR20_12700"
     CDS             complement(15757..16236)
                     /locus_tag="VFR20_12700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0231420.1"
                     /translation="MALEAHPLRDTIRIVIGDWHALESHATAIRTEVFVAEQHVPAEE
                     ELDAEDVHCVHAVAYDGALGVGTGRLLPNAHIGRMAVRKPYRGRGVGARLLETLVVEA
                     RKRGYPEVILAAQLHAVPFYERHGFVAEGGVFLDAGIEHVNMRRVLAGASSRDLSGA"
     gene            16395..17699
                     /locus_tag="VFR20_12705"
     CDS             16395..17699
                     /locus_tag="VFR20_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015105 - arsenite transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic transporter"
                     /protein_id="HEU0231421.1"
                     /translation="MPPLPAIAAVLIFIGTLLLVIFRPWKLGIGWSALAGAVACGALG
                     LIHLSDVREIWHIVWNATATLIAIIITNSLLDEAGFFKWAALHIVRWGRGRTSILFVL
                     IILFGATVAGLLTNDGAALILTPIVMELLLALEFPAPAIFAFVMSAGFISDTASIPLT
                     ISNLVNILSADYFGLGFRGYAAIMIPVDVVAIAASLLVLHAYFKRRMPRSYHFARPMP
                     PAEAVRDPLTFHAGWMVLLATLAGFFTLDRYNVPTSVVAGAGAALLLVVAGRRHIVGT
                     RKVLRHAPWNVVVFSLGMYLVVMGLRNTGMTSWLSDLFRLFAGSGIWGAALGVGFVSA
                     FLSSVTNNLPGVLLGMLSIHDAGATGAVHQAMVYANIIGSDLGPKITPIGSLATLLWL
                     HVLARRGTHVSWGAYFRTGIVLTVPVLFFTLMALALSLQLVR"
     gene            17937..19625
                     /locus_tag="VFR20_12710"
     CDS             17937..19625
                     /locus_tag="VFR20_12710"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEU0231422.1"
                     /translation="MKHQLFTRHTAFYLIAAAALLCLILTVAVSDWWLILTIVFGALT
                     VLGIIDLTQTKHAIRRNYPVIGNLRFALEFIRPEIRQYFFEDDTQKLPFSRVDRSLVY
                     QRAKQQIDKRPFGTQGDVYQTDYEWINHSMTPTHPANSDFRITVGGPDCTQPVSLSVL
                     NISAMSFGALSANAVMALNKGAATGNFAHDTGEGGVSRYHLAHNGALIWNIGSGYFGC
                     RDTAGNFSEEEFVKQATRDNIRMIEIKLSQGAKPGHGGILPGPKVTPEIAAARGVPAW
                     QDCNSPSAHSAFDTPIGLMHFIARLRNLSGGKPVGFKLCVGHPWEWFAIAKAMLETGI
                     TPDFIVVDGAEGGTGAAPIEFVDHVGTPLREGLRLVHNTLVGIGLRDRIKLGASGKII
                     SAFDMARIMALGADWCNSARAFMFAVGCLQAQVCHTGRCPTGVTTQDPKRQKALVVAD
                     KSVRVANFHTNTLKALAELLGAAGLTHPGDLRPHHIARRIANGEIRVLSALFPDLENG
                     ELLAGKFRNTVFRTAWPMARAESFDPAYSLSAALSGHLDISIPEDTHAGQARVN"
     gene            complement(19753..21024)
                     /locus_tag="VFR20_12715"
     CDS             complement(19753..21024)
                     /locus_tag="VFR20_12715"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0231423.1"
                     /translation="MMLRRIFSWLSAASLLLFSTLAAATPAPQAPAEIKIGALYASSG
                     PFAAISMPAHMGLKLWVDEVNAKGGVMVKPYGKRIPVKLVAYDDQSNTSTAATLYNQL
                     ITQDKVNVLVADVGSVLTSMAVPIAREHKMMLFDLSGTGDPFFTPGNPYIALVAMPVS
                     TVWPKSVANFLTTEGPKLGIKHIALIYATNDFSGTQAKAMRKLIEDAHAPVKIVYDTG
                     VPTNTSSYTVLLNSIRAAKPDAVIQLGYPGNDTAFLRNLHDSGAHFKWLFAVFPGLET
                     EHMLKTVGVDGMKYVYTYTPPSEVEYKVTSGMTLPEFHTAWDKAYPDSKVSFGFNSVA
                     GYTTGVVIQNTLAATNSLDQLDLRKAVFSLSGKLKTLDGAFELDKDGAQTGEVMPLGQ
                     LVPDGKGGLKFSVVYPAEAATGKPIYPAPAQ"
BASE COUNT         4154 a         6641 c         6313 g         4017 t
ORIGIN      
        1 tgccccatgt cggcgtacaa ggcctcggcc ccggtcagcg ccagcaccac cgcccccagc
       61 agcagaaagg tttccaacgg accgtcggcg atgagtcgcc atgcatagag gggattgagc
      121 gccagcagta tcttgggtgt caaccagatg cgccacgcac cgagcacgcc aagcgtgacg
      181 aaccacagca gcatgatggg gccgaagagc ttgccgactg ttccggtacc gcgcgactgg
      241 atcaggaaca aggcgaccag aacggccaca gaaatgggca cgatcagctc atcgaactca
      301 tgcgacacca cgccgatgcc ctcgacggcc gaaaggacag aaatggccgg cgtgatcatg
      361 ctgtcaccgt agaacagcgc cgcgccgatc agccccagaa cggtcagcac catgccgcgc
      421 cgccgcgtca tgccgcgcac cgccaattcg gtcaacgcca gcgtgccgcc ttcgccccgg
      481 ttgtcggcgc gcagaatcaa cgtcacgtat ttgagcgaaa ccaccagcat gagcagccag
      541 aacaacaccg acagcacacc cagaatgtgt tcgtggctgg gcgtgccata ttgagcaaga
      601 gacaccttga tggtgtagag cgggctggtt ccgatgtcgc cgaacaccac ccccagcgcc
      661 cccaccagaa tggccgcccc cgcagcgctc tttgtcttgg aagtgtcgtt cacttctgtc
      721 gttctcccca tttggctact cttgtctggt tagtctcgcg ccgatgcgtg gccccgggaa
      781 gacaacactg atgcgcgtac ccggaaaatc cggccggctc agcaggctcc accaggcgcc
      841 atgcgcccgc gctatttcac gtacgatggc caggcccagg cccgaccccg ccttgttgac
      901 cttgggcgac cggtagaacg cttcgaacac gcgctcactg tcggccgggt caataccggg
      961 gccatcgtcc tcgatgacga gtgatggtgg attcggcgtc actttcaggc caatcacctt
     1021 ggctcccgtg ccataacgca atgcattgtc tatcaggttg cccagcaatt cctcgagcag
     1081 aagtgggtcg gcatcgatcc agaccggttc atccggcgcg gaaagctgaa tatccacttt
     1141 gtgtgcccgg gccgcggcga tccactccgc cccgttgatg cgcgcccact cacagagatc
     1201 ggtcctgaca aagctgtctt gcgggcgcgt tgacgaatca gcgcgggcca ggaccagcaa
     1261 ttgctgccct agccggatca tgcggtcggt gaccgacttg atacgctcgg cgcgcgccct
     1321 gacgtcgtcg ggcagggggc gccccagcat caattccgat tccagcttga gcccgctgag
     1381 cggcgttctg agttgatgcg cggcatggcc gataaaacgc ttctgggcct gcatagagtc
     1441 atccagacga accagcagat cgttgaaatg ggtgaccacg ggggccacct cggtgggcaa
     1501 cccggcgatg tccagcggct gcagatcatc gatggaccgt tggctgatct catcggacag
     1561 gcggttgatg gagcgcagcc ccgagcgcac gcccgacacc agcacccagg caaacagcga
     1621 caccagcaac acctggctgg tcatcatggt ccagaaaatg tcgcgcagca cccaggcttc
     1681 caggcccacc ttgcgcgtga ccacccaggt aacaaccaga tcgagcagaa tcaaggcgat
     1741 caacgcgggc aacaaccgca tcaccagatg acgcgccagc gacccttccg gcaccagcat
     1801 agtcaaacgc gagcgtggca atcgtgattt tctggcgagg gatgggccgg gatcagaaaa
     1861 catgggcgtg tgctgcggcc cccgcagcgc ggcggaagca tggcaagcgc ggagcggacg
     1921 tgtcaggccg cactttccac gtcgagcaag tatccgaacc cacgcacagt ctgtatgctg
     1981 gcgccagtgc catcgagccg cttgcgcagg cgatacacat agacttcgac tgcgttctcg
     2041 ctgaagtcgg catcccaggc cgagagagaa ttgacaatct gatgcttggt gacgacacgc
     2101 cccacccggc ccatgagcat ttccaggacg gacagttcgc gcaccgacaa cgccaggcgc
     2161 tcaccgttga cgcggacttc tcgtccgacc gtgtcgaaca tcagtgggcc ggcctcgaga
     2221 aatggctggg cctgcccggc acggcgtctg gcgaatgccc gcacacgggc cgcgagctcc
     2281 gggagctcga acggcttggt gacgtagtca tcggcccccg cgtccaggcc ggccacacgg
     2341 tcttccacgc catcgcgggc agtaaggatg agcacgggcg tctgctggcc gccgctgcgc
     2401 agttgccgca gcacgtcgag gccgctcatg ccgggtagat tcaggtcaag aagcagcaaa
     2461 tcgtaggtct ggccggccac ggcccccaga acctggtcgc catccttcaa ccagtcgacc
     2521 gcatagcctt gttcgaccag gaattcctgc agggcatggc ccaaagtggt gtcgtcttcg
     2581 attactagaa ctcgcatggc gtgattctat atcgaacgcg gatatttttt gtaacggcgg
     2641 cactgcgaca ccttgaaagt tacacgttgg aaacattttc ggggcggaaa atcacgggga
     2701 cgcgcccgat ggctgcgtga cgaaacccag gcgcgtcaca ccggcggccc gcgccaggcc
     2761 catgatccgg gccaacacct cataacgcgt gttcaggtcc gcgcgcagcc gcagtctcgg
     2821 ctgtggattc tgtgtagcca tggtgtcgag gcgctggccc aactggctca tgtcgacggg
     2881 ctcgtcgttc cagaacatgc gccccttggc atcgattgcc aagtccacgg catccggttc
     2941 cgccgctgtg ggttgcgcgc tgacgcgcgg gacgtccacc ttgatggagt gggtgagcaa
     3001 cggcgcggtg atgatgaaaa tcaccaacag caccagcatg acatcgatca ggggcaccat
     3061 gttgatttcg gacatggtct gcccgccgcc cttgttctcg aaaccgccga aggccatgtc
     3121 agtgcccctt gcccgacgcc gccagggcca ccgggcgggc cttgccggaa ttttcggcga
     3181 cttgctggcc ggtgatcaaa aaggtgaaga ggtcgtgcgc aaacgcatcg agccgagcca
     3241 gatagacacg attaccgcgc acgaacccgt tataagccaa cacagcggga atcgccaccg
     3301 ccaggcccag cccggtcatg atcagggcct cccccaccgg gccggcgata cggttgatgc
     3361 tgactccatc agacaagccg atgcccacca gcgcatgata gaccccccac acagtgccga
     3421 acaggccgac gaacggcgct gtggagccga ttgacgccag gatggtcagg ccgttttcca
     3481 ggcgcgctgt gtcctcgtcg aggaccttgc gtatggtgcg cgtcacgaag gcactggtcg
     3541 aaccggtctc ttccagcttg agggcgccat acttggcatg atgattgcgc gcgtggatgg
     3601 cgtggctggt caagtgggag aacggttcgg tgacgccgtg ggcggcaatc tcgttctcca
     3661 cctgttcgag cgagctggcc gcccagaact cattcaggaa cttggtcgag cgtcgacgat
     3721 tacgcatcgt catgaagatc ttgacgaaaa tcagatacca gctcaccagc gacatcagca
     3781 gcaaaatacc aaacaaggat ttccccaccc agtcgctatg ggacacgaaa tcgatgacgc
     3841 cgctcgtctg ggtcgatgag cctccaccag ccgccacggc cacgatcgcc ggcgccgcag
     3901 cctgaccgac ctgggacagt acttggatcg cactgtgtag aacatcgaac atgatggctt
     3961 cctattcttt tatatataga acttttatct atagaacaaa atcgattggg atgtcggccc
     4021 gggccgaata tgcgacacca ttttcagtgt agggccggaa ccgcgccctg cgcacggcgt
     4081 cgagcgcggc ggcatccagc aatgcgtgac ccgacgatct ttcgaccgtg gctttcacca
     4141 cattgccctg cggcgaaatc acgacgcgga tgacgacctt gccagtctgc ccccgccgca
     4201 acgccgcgtc ggggtaccgg ggggttggcg gacggccctg gtaagcaacg tgggtcaccc
     4261 agcgtggccg cgaccccgcg ggtgaagtct cacgcgccga ctgaccacct gtcgccccgg
     4321 aacctgtcgc caatgccact gcgtgactcg cgttcgcttg atcggcaaga ttggacggct
     4381 ggccccgttc cccctctatt gaggccggtt tcgcgacgga ttgtttccgg gcgacgggct
     4441 tgggggcggg tttggacaga ggatttgaat caggccggga tatgggtttt gactcggatc
     4501 gacgagagat cttgcccttg ttgtctctgg gctcgcgacg tgaggcaggc ttgactctat
     4561 caaaagtggg tttgctttcc gtgtgtgccc gttcgcgctg cgccgaagcc tgatcgggtt
     4621 gaatgttggc ttcggctcgc cgcgctactt gcacacgaga tgtggttgaa ccgggtttcg
     4681 catcggaatt caccgttgac tccgaaccgg gcgaaggggc cggcggcaag agctccacca
     4741 tgctgacctg catcaccgaa tcgaggcgag cgggatcgac gccggatggc tgtgccagga
     4801 taaccacgac gacagccaag tgcaggccaa gcgcgcagac gatcgccgcc gccttctcca
     4861 gtaatgccca tctcgattcg gaacggggta ggaacatgaa acttgaaaaa tgaatggatg
     4921 gctcgaaccg atatcaagcc tgcagtcgcc agcatcagac aggatgcgcc agtgcgcagc
     4981 ctggttgact cggtttgaat gcacgaacca accaaaccgc tcactgtgat aataaaggga
     5041 tccagggcag gcgcacaccc accatgacaa tgacgtcaga acgcactcct tcacttcaca
     5101 accgccggcc gcccactgga tggggaacta cccacctttg cgctgctggt tcggcgtctg
     5161 ccagataaac cgatccatct gccgctgggt attatcattg tccatttcct gctgcttgag
     5221 caggcagttc gagtaggcgt cggtaccgct cttgaaaccc agttggtcac actgcttttg
     5281 atattcctgc aacgacgcct gatcgagtcc gtgatgcaag gcgcaaccac tgagcaatga
     5341 cacggcggca cacacgacga caaatccgga tttcacgaga actcctccat ttgaaaaatg
     5401 tttttgccag agttttgcta gagataagaa aaaagccgtt caagtgcccc tgaatggctt
     5461 ttccaatgtt gtttcttagt ggtgctacat catcgcaact gtctctggaa agcagcataa
     5521 acacttggag tgatgaattc accattaaga aacaccgcca agaataccgc ccgcgaaacg
     5581 ttgctgagcc ggtcggtggc caattatcgc ccaggccggg gagcgttgtc gacatcgcta
     5641 tcatcgccat caccgcgcgt acgattggaa cgtccccgca atttttcgga aacaccgcat
     5701 tcatccgctg ccaacacagt ccaccacgct tatacaagga aagcacatga cgagcccagg
     5761 taacccgtac aatccgtttt cctcccatcc tccgacccgt agcagtcgcc ggcgatttct
     5821 cggccacacc ggtgcgggga tgctggccgc ggtgctgccg ggtcgcgctg gagccaatgg
     5881 ccaactggca tcgtcgtata gccagaccat cgcgtacggc cgacaagcga tccagcaact
     5941 gatgagcggc aaccagaata tttccactat ttcggtcgcg ctgctcaaga gccagcagat
     6001 cgtctggcag caggcgttcg gcatggcatc gatgacgaac agcataccgg ccacactgca
     6061 aacacgctac aacatcggtt cggtcagcaa agtgcttgcc gcgctggtgg cgacgatcct
     6121 ccaggaccgc gggctcatca gcctggaaac accgatggtt cagtacctgc cagcctttac
     6181 gatgctgtcg ccggagtaca aacgaatcac cgtgcgccat ctgctgtcgc acacctcggg
     6241 ttttcctggc accaatggac gcaacgcatt catgcttggg gtgccggcca ccggatacgc
     6301 ggccgatacc gaggcggcgc tagcgaacca gcacctgaag catttgccgg gcgagctggc
     6361 ggtgtattgc aacgacggct ttacgatgat cgagcagatc gtgcttgccg tcacggggca
     6421 gccctatgcg gacttcgtac aggagaacct tctcacgccg ctggccatga cagacagtgg
     6481 tttcctgacc agcgtgccga gcgtcggcga atatgcgtac ccctggctca atggcaaaca
     6541 gtatgcgcag gagtttgtca gtccgctggc gtctggcggg ctctccacca cacccactga
     6601 catgatgaac ctcgcacgga tgttgattgg cggcggtctg ttcagggggc ggcggattgt
     6661 ctcggcggca ggcattgctg aaatggccac ggaccagacc accaagctgt tgatcaaccc
     6721 atctccagaa tggcggtggg gcctcggctg ggatagcgtg catcaactgg ggctcgccgc
     6781 ggccggtgtc aacgcgtggc agaaaggcgg gaactcggca tttttcgaga cccagttcat
     6841 ggtgctgcca gatgcgcaaa tggccctgat ggttacgggc aataacggtt tcggtgcaga
     6901 gaagctcgcc gagggcattc tgatcaaggc gctgctggag cagggtgcgt tagccgcggc
     6961 accagccagg gtttctgata tggcaccacc ggttgcgccg gcaccgaatg tattggacgc
     7021 ggcaggcatc tacggcaact cttctgtgcc gagacaagtg atcgttgcgg cagacggtgg
     7081 cttcacgctg aacaaatgga acggcaccac gtgggtgccc gatggctcag cgccatacca
     7141 gtaccgcagc gatggctggt ggtggagcac cggacaaggg ccactgagtt tccgcttcga
     7201 agtggtggcg aatactgatg aggagggcaa gccgttcagc taccgctatc tgatgatgcg
     7261 ctatgtgact ggcgccggct ataaccacat cacgctgcca atgggccagc agttgagcgg
     7321 cccgcaactg cccgcgctcc cggcgctcga tcccgtatgg caggcgcgta tgggcatcat
     7381 atggaacctc accaaccagt cgcctgattc tttgccatcg cagttttcag gtccattgca
     7441 ggcaaccttg agtgagcccc cctggctaca aggatacatt ctattacaca acaatgacta
     7501 cgacttgggc gccccacaat accaactact ggtgccactc gcgaatgatc gcggcggcat
     7561 ggcggtcaag gtgcctgtca tccaggggcg cgatctacag gaaatctctt tctcgagcgt
     7621 gaacggggtg ccgacaatga caatcgggag ctggatatac actgcggcat gaagtgatca
     7681 ataaaaaacc cgcaaggcca gaaccatgcg ggtttcatat acggctgtgg actgctcggc
     7741 acttaagttt ggtgggtgct acagggttcg aacctgtgac ctacgccttg taagggcgcc
     7801 gctctaccaa ctgagctaag caccccgtcg accgaccaag cggccggccc ggtgccgggc
     7861 ggcttgccag caagcactga gctgcggcaa ggcgccattc taacgggcgc cggctggcga
     7921 tgcaaaccgg cacccgcggc acagagcctt agttgacggc gtctttcagg gccttgcccg
     7981 gacggaattt tggcaccttg gccttcttga tcttgatggc ttcgccggtg cgggggttgc
     8041 ggccggtgcg cgcggcgcgc tcggaaacag caaacgtgcc gaaaccaacc agagtcacgg
     8101 aaccgccctt cttcttcagg gtggtcttga cggcgccgat gaaggcttcc agggcacggc
     8161 cagcatcagc cttggagaga tccgccttgg aagaaatatg ttcgataagc tcggttttgt
     8221 tcattaagga gtacccctga aaattatttt agccagactt ccgcatccgg acagtgcctg
     8281 gcgctagacc acgaaaacac gccgcacacg agttgccgca tgtgacccac aatagacatg
     8341 ttgcacgcaa ccgggtggac aaggcgctgc attcgaccac ctgattggct aacgcgtatt
     8401 aagcctttga aaaaaagcca tgtcaagaga gcgatcgtcc aaaacccgca taattgctga
     8461 cttttttgca tttgcgccgc aaccacgaca aaatttgtca gggaatgcgt gtggcacagc
     8521 tgatctgtga taagcgttcc cgagcgtggc tatggagagc tctcgcgcca tacgaaaggc
     8581 ccgggaaacg ctggcctcag aaccgggcga gcgcctgggt gaagtcgcca atcaggtctt
     8641 cggtgtcttc gatgccgacc gacacacgca ggaaggtatc agagatgccc atgagcgcgc
     8701 gttgcttcgc acccatttca tagtagatgg tctgcgccat gggcagcacc agcgtgcgtg
     8761 tgtcgcccag atgtgtcgcc agcaccagca catcgagatg gttcaggaac tcgaacacgt
     8821 cgatgccttc aaccagatcc acccccatca gcgcgccgaa tcttccaccg aaatagtcgg
     8881 ccgcgcgctc atgctgggga tgtgacgcca agcccggata atgcaccttg gcgaccttgg
     8941 ggtggctgtc gagaaaccgg gcaagcgaca gggaattggc gcagcacttg tccatgcgca
     9001 aggccagggt ctcggcgccg gccgcgatgc gatgggcggc ctcggatgac aacgtgcccc
     9061 ccatgtcgcg caaacccttt ttcttgatct gcgtcagccc ccaggtgttt cctggacctt
     9121 tgcggtaggg ttccgcgatg ttcgggtacg tggaccagtc gtacaggcct gtgttggtca
     9181 ccgctccacc cagcgcgttc gcgtgcccgc caatgtattt ggacaaggaa ttcatcacca
     9241 gtgacgcacc gaacgccttg ccggtgctga gccagggtgt ggacaatgtg ttgtcgatga
     9301 catagaccag attcttctcg cggcaccagt cgccgatggc gcgcatgtcg gccacctggg
     9361 tgccgggatt ggcgatggac tcggtgaata ccatccgggt ttccgggcgc caggcctgcc
     9421 tgacgtcatc gatgtttgtc gcatcgacca gcgtgacctc aacacccagg gccgccaggg
     9481 tgcctagcag gctgttggtg ttgccgaaga tgtatttgct ggaaatcagg tggtcaccac
     9541 gccgcagcag cgcgaaaaat gtggcgttga gagcggccat ccccgtggcg aagctgatgg
     9601 tcgccaagcc ttcttccatt tgcgtgacct gggcctccag ggcggcggtg gtgggcgttc
     9661 cctgccgcgc gtaggtataa cccggtttgc cttgaaacac cgccgccagt tcgcgcgcgt
     9721 cggcgtaggc atattgcgac gacgggtgca tgggcttgtg cacagcgccg tgctcggcgc
     9781 cagcgcggcg atcggaatga agaatggttg tggtgaagcc gttttttgac atgatggaag
     9841 caagcgtgac gaggaaagcg ctgaagggaa gccgttatta tgcgccttcc gttcatggct
     9901 cccacgccgt atcagcccgc cgccatccgt tgacgcaccg tttcgtaaag gcacacggcg
     9961 gtggccacgc tcacgttcag actttccacc gaccccagca tgggaatggc cacgcgctcg
    10021 tcacaggttt cgcgggtgag ccggcgcatc ccctcgcctt ccgcccccag gacccacgcc
    10081 atgggccgac acgcgtccac gccatggaac cgcgtatcgg cctggtcatc ggtgccgatc
    10141 agccacaacc cgcgatcctt cagatggcgc atggtgcgcg cgagattggt caccatgatg
    10201 tagggcacgg tttccgcggc tccgcaggcg acacgcgcca cggtggcgcc aagccccacg
    10261 gagcgatcac gcggcgcgat gaccgcgtgc acgccggccg catccgccgt gcgcagacaa
    10321 gctcccaggt tgtgggggtc tgtcaccccg tcgaggatca acagaaacgg ggcgataccg
    10381 gtgtcttcca gcccatcaag aacctcatcc acgtcgatgg cgagtttctg ggactgcgcc
    10441 agggcaacca ccccttggtg acgcgtgcca tgggccaggc catccagcct ttcggccgga
    10501 acagggtgca cacgcacgcc agcggcttgc gcctgctcca acaacgacaa catgcgtttg
    10561 tcgcgccgcg acgcgtcgac gtagagatcg acgatggacg ccggcgcgtg acgcatccgc
    10621 gccacggccg catggaatcc ggcgagtacc tgatgagacg acataatttc ctcgatgacg
    10681 acgcggcggc cgtccaaagg ccggccgcgt cctgtgcttt cactgtgcgg cgttctggtc
    10741 gtttacgcgc tatggtcgtt tacttacggc cccggtgccc actctttttg ccaacggcca
    10801 gtgccgacgc ccgggcattg cgcttggcgg ccgcccttcg ttcgcgcgac ggccccttga
    10861 gagagtctgg cttggccgac gccgcgcgct tcacccgatg aggcgtgccc gactcgggtt
    10921 ggctcaatgc cttgaagctg gtgcctttga ccagcctgaa ctctatgcgg cgcgcctcta
    10981 ggtcgacccg ggccacctgc acctgcactg cgtcggtcaa ccggtaacgt atgccggtgc
    11041 gttcaccacg cagttcgtgc atggcttcgt tgaactggaa gtaatcggaa cccagttccg
    11101 aaacgtgcac catgccctcg acgaacaagg tgtccagcgt gatgaacacg ccgaagctcg
    11161 cgacaccggt gacgcgcccg ctgaagactt ctccgacgcg ctctttgacg aaccaacact
    11221 tgagccacgc ctcgacatcg cgcgaggctt cgtcggcgcg acgttccgat gccgaaagca
    11281 gcaggccgag tttttcccag gcagcatgtt cgcgatcgtg cttgggaagg ctcgccaccg
    11341 cggctgcgtc gtcgaccttg ggcacataac gcgtgccatc gatgatggac ttgatgacgc
    11401 gatgcgtcag cagatcggga tagcggcgga tgggtgacgt gaaatgcgca taatgttcgt
    11461 acgacagacc gaagtgcccg atggtgtcgg ggctgtaaat ggcctgctgc atggagcgca
    11521 ggcacatggt ctgcaccacc tcgaaatcgg gtcggttgcg cgccgactgc acgagccgcg
    11581 cgtagtcgtg cgtggtcggg tcgtcaccgc cttccaggga cagccccagg ttgcgcagga
    11641 aatcacgcaa ggcctgcagc ttttcgggcg tcggcccttc atgcacacgg tacaacccca
    11701 tgcgcttgtt gcgcgtcatg aaatcggcgg cgcaggtatt ggccgccagc atgcattcct
    11761 cgatcagccg gtgggcatcg ttgcgcgtat acggcacgat cttttctatg cgccccaatg
    11821 ggttgcagag aatcttggtc tcgacggtgt cgaaatcgat ggcgccgcgc cggacacgcg
    11881 cctcggccag cagttgatac agttcataga ggtcgcgcag atgcggcaac acctcggcca
    11941 tggcctgggc cgccggcccg tccggttgct gcaaggccgc ccagacatca gtgtaagtcg
    12001 tgcgcgcatg ggaatggatg acggcgttgt agaactgata cgccgacacg gtgcccgcct
    12061 tctttccgcc caggggaatg aacatgtcgc agaccatgac cagccggtcg acgtccgggt
    12121 tcagtgagca caggccgttg gaaagcgttt ccggcagcat ggggatcacg cgccggggaa
    12181 aatacacgct ggtgccacgt tccacggcgt catcgtcgag cgcgtcgccc ggccgcacgt
    12241 aatggctgac atcggcgatg gccaccaaca ggcgccagcc cttggtcttg cgcgcgccat
    12301 tgcccgattc ggcgggcagg cagaacacgg catcgtcgaa atcgcgggcg tcctcgccat
    12361 ctatggtgac gaatggcaga tcacgcagat cgacccgccc cggcaattcc gccggtctga
    12421 ccttggcggg caaccgcgcc gcctgcttga gcgccgcgtc ggaaaaatcc agcggcacat
    12481 cgaacttgcg caccgcgatt tcgatttcca tgccggggtc atcgatttcg cccagcactt
    12541 ccaccactcg acccagtggc tgcgtgtgac gcgtgggttg ctgcacgatt tcaacggtga
    12601 ccacctggcc gttctcggca ccgcccgctt cgccgggagg aatcaagatg tcgtgtttga
    12661 tgcgctggtc ttcgggcgcc acgatggtgg cgccgtgttc cttcagaaaa cgccccacca
    12721 gccgattggt acgccgttcg acgacctcga cgatgaccgc ctcgggtttg ccccggtaca
    12781 tgccgtcggg cttggccagc acgcgatcgc cgtgcagcac tttgagcatt tcacgcggcg
    12841 acaggaacaa gtcgggtccg ccgtcgtcac gcagcagaaa gccaaagccg tcgcgatgcc
    12901 cttgcacgcg gccggcgatg aaatccgatt tgttgttaat aagcaactgg ccacccggat
    12961 tgcgtaatag ctggccatcc cgctccatcg ccttgatacg acgctcgaaa cccacgggaa
    13021 aaggctccga cacacccact tttcgcgcca gtgcctcagg ggaaagcggc tgtccagcca
    13081 tacgcagttg cgtcaggatg gcttcgcggc tgggtacgtc ggggtcgaaa tcaggggggg
    13141 tttcaggcag gccattgagg atctcgttgt tattgatttc gttcttggat cgtttggtca
    13201 aaagtagatt tccgttcaga taaaaattcc gctatacttt cgcttctttc aaagaaagag
    13261 cagcgctggt accggataaa agcattagca tactctttta agaaaagccc aggtggcgga
    13321 attggtagac gcgcatggtt caggtccatg tgccgcaagg tgtggaggtt cgagtcctct
    13381 cctgggcacc acaagttgtt tcgacacagt tgtttcggca acacccgaag aaccccgtaa
    13441 ggccacggct ttacggggtt ttttgttgag cctggcgcat taacccgcta ccattcatgg
    13501 ctgcagtgtg ttgtttggtt ggttcgtgca ttcaaaccga ccgaacagga caccaaccag
    13561 gacaccaacc aggacaccag cccatttacc tggacgcggg caacgcccgc gctgtctgga
    13621 tgccccactc gccgaagagt acaccaccat gctagcctcc ctgggaaaac gcctggcccc
    13681 gttgctgctg tcgatcatcg catcgggcgc gctcgcacaa acgcctaaaa cggtattcct
    13741 gcaggatctg acctggaccg aactgcgggc cgagatcaat gcgggcaaaa ccaccatcat
    13801 cattcccatt ggcggaaccg aacaaagcgg tccggatatc gcgctgggca agcacaatgc
    13861 gcgggtccgg gtgctgtcgc agcgcatcgc gcaaacgctg gggaatgcgc tggtcgcgcc
    13921 agtgatcgcg tatgtgccag agggcagtta cgcgccgcca accgcgcaca tgcgatttcc
    13981 cggcaccatc accattccgg acgacgcctt cgagaaaacg ctggaatccg cggccaacag
    14041 cttcaagatc cacggcttcc tgaatatcgt tttcctggga gaccacggcg gctatcaaaa
    14101 cgatctgaaa ctcgtggtcc accgcctcaa tcgatcatgg tccggcacca aggcgcgagc
    14161 gttcgtgccg cccgcatact acggcaccag ctcccgcggt tttgcgcaaa tcctcaggaa
    14221 acggggttat cacgacgccg aaatcggcac gcacgctggc ctggcggata catcgctgct
    14281 actggcagtg gcgcccagca tggtgcggct cgatcgcctg cgcagcggtc cgacgcccgg
    14341 agcggcggaa ggggtgtatg gtgacccgcg ccgcgccagc gccgaactcg gacagttggg
    14401 ggtcgaggcg atcatctcgc gcaccgtcgc ggccatccga aaagacacgg caggccgatg
    14461 aacaccgatt cccttgtttc cgctcccttg cggtcttgat tcccgccttt tgacaaactc
    14521 ctggatggaa tttctcatgt actcactgtg ctttcccgcc cgccgccgcg ccgcgcgttt
    14581 cgtgccggcg cttggtctcg tgttggggat actttgcgcc ggaccagtgg cgtccgccgc
    14641 caccagcatc gacactgtgc cgggcatgcc cccggtggtg aatccgaaca acctgtatag
    14701 cgaagccggc gtcaatcatt tcagcccagc ggtggccggc gccctgtcgc gcgtctatgt
    14761 gcccaacgtc cgatccaatg atgtgtacgt cattgacccc gcgacgttca aggtcgtgga
    14821 taaattccgc gtcggccgca acccccagca catcataccc tcgtgggatc tgaaaacgtt
    14881 gtgggttgcc aacaatgacg aaggacgtcg tggaggaagc ctgactccga tcaatccgaa
    14941 aaccggcaag ccggggcccg cgataccggt cgaagatccg tacaacatgt acttcacgcc
    15001 ggacggcaaa tacgccatcg tggtggccga ggcgcttgcc aggctggact tccgcgatgc
    15061 gcacaccatg gcgttgaaag tgagcctgaa agtacccgga tgcaagggca tcaaccacgc
    15121 cgatttttcc atcgacggca gatacgcgat cttcacctgc gaattcgacg gcagcgttgc
    15181 gaagatcgac atggtcaatc gcaaggtcgt cggctacctg cggttcgcca aacccagcat
    15241 gccgcaggat atccgtattt cgccggatgg caggatattc tacgtggcgg acatgatggc
    15301 caatggtgtc cacctgatag acggcgatac cttcaaagag atcggtttca tcaaaaccgg
    15361 tgtgggtaca catggcctgt accccagccg cgatggcacc catctgtacg tctccaatcg
    15421 gggctcgaat ctggtgcacg ggccacgcca tggcaagggc agcgtatcgg tcatcgattt
    15481 cgccacacgc aaagtcgtgg cgaactggcc gatccctggc ggcggcagcc ctgacatggg
    15541 caacgtcagc gccgatggca aaatgctatg gctatccggc cgtttcgatg atgtggtcta
    15601 cgcgatcaac accacgaccg gggcggtgaa atccattcct gtcggcatgg agccgcacgg
    15661 cctcaccgta tggccgcaac cgggccgtta ttcactcggg cacacgggca acatgcgcta
    15721 atgcggtgtt tgacgctatc agggacataa accgcactag gcgccagaca aatcccgaga
    15781 gctggcgccg gccagtacgc gtcgcatgtt gacatgttcg atgccggcgt ccagaaacac
    15841 cccaccctcg gcaacaaaac cgtgacgctc gtaaaacggc acggcatgca attgcgccgc
    15901 gaggatcact tcgggataac cgcgcttgcg cgcttccacg accagggttt cgagcaagcg
    15961 cgcgcccacg ccccgccccc ggtacggctt gcgcacagcc atgcgtccga tatgggcatt
    16021 gggcaacaag cgccccgtcc ccacaccaag cgcgccatcg tacgccacag catgaacgca
    16081 gtggacgtct tcagcatcaa gttcctcttc ggcaggcaca tgctgctcgg ccacgaagac
    16141 ttccgtgcga atggcggtgg catgcgattc gagcgcatgc caatcaccga tgacgattcg
    16201 aatggtgtcg cgcaaggggt gagcctccag agccatgacg gtatcaagaa aaagggcgaa
    16261 aaccgcatgc ggccattcta ctcaaaacgc accgcttccc gtttcggtga taattgcggc
    16321 aaacgcgata atgccctttg cctgttcccc tgatactttc ctcccctacg ctgatatctt
    16381 ccccagccct tctcatgccg cctcttcccg ccattgccgc agtgctgatt ttcattggca
    16441 cgctcttgct ggtgatcttc cggccatgga aactcggcat aggctggagc gcgctggcgg
    16501 gcgccgtggc gtgcggggcg ttggggttga ttcatttgag cgatgtccgc gagatctggc
    16561 atattgtctg gaacgcgacc gccacactga tcgccatcat catcacgaac tcgctgctgg
    16621 acgaagccgg tttcttcaaa tgggccgcct tgcatatcgt acggtggggg cggggccgca
    16681 catcgatcct gttcgtgctg atcatcctgt tcggcgcaac cgtggccggt ctgctgacga
    16741 acgatggcgc ggcccttatt ctcacaccca tcgtgatgga gttgctgctc gccctggaat
    16801 tccccgcccc ggccatcttc gcattcgtca tgtcggcggg gttcatctcc gataccgcca
    16861 gcatcccgct cacgatctcg aatctggtca acattctgtc cgccgactat ttcggcctgg
    16921 gctttcgcgg ctacgcggcc atcatgattc ccgtcgatgt ggtggccatt gcagccagtc
    16981 tgttggtatt gcacgcctat ttcaaacgcc ggatgccacg ctcctatcac ttcgcgcggc
    17041 ccatgccacc cgccgaggcg gtgcgcgatc cgctgacgtt ccacgccggc tggatggtgc
    17101 tgctggcgac gcttgcggga ttcttcaccc tcgaccgcta caacgtgccc acgagtgtcg
    17161 tggctggcgc cggggccgcg cttctattgg tggtggccgg acgacgccac atcgtgggca
    17221 cccgcaaggt gctgcggcac gcgccgtgga acgtggtggt gttctcgctg ggcatgtacc
    17281 tcgtcgtcat ggggctgcgc aacaccggga tgacatcgtg gctgtcggac ctgttcagac
    17341 tgttcgcggg gtcggggatc tggggcgcgg cgctgggcgt gggtttcgtc agcgcgtttc
    17401 tgtcgtcggt gaccaacaac ctgcctggcg tgctgctggg catgctgtcc atccacgacg
    17461 cgggcgcgac cggcgcagtg catcaggcca tggtgtacgc gaacatcatc ggcagcgacc
    17521 tgggtcccaa gatcacgccg atcggcagtt tggcgacgct gttatggctg cacgtgctgg
    17581 cgcgacgcgg cacccatgtc agttgggggg cctatttccg caccggcatc gtcctcaccg
    17641 tgccggtgct gttcttcacg ttgatggcgc tggcgctgag cctgcaactc gttcgctgac
    17701 catatacaag ataggcgtgg tgccataaag ctctgggact ggcccacggc cctcgcagtg
    17761 aacctggcca tcctcggcct gatctactgg ctcagcatcg tgttgcgcgc ccccccccgc
    17821 actctgtcca tcggactcat ggattcctca ggcgccggac gccgcatcgc ccatgtattg
    17881 gggcgcaggc gaagttctgg taaggtgatc ggctgataca gcctgcggac ccgtccatga
    17941 aacaccaact gttcacccgc cacaccgcgt tctacctgat cgccgccgcc gcgctcctgt
    18001 gcctcatcct gaccgtggcc gtgagcgatt ggtggctgat cctgaccatc gtgttcggcg
    18061 ccctcaccgt gctgggcatc atcgacctca cccagacaaa acacgccatc cgccgcaact
    18121 atccggtcat cggcaatctg cgtttcgcgc tggaattcat ccgcccggag atccgacagt
    18181 atttcttcga agacgatacg cagaagttgc ccttctctcg ggttgatcgc tcattggtct
    18241 accagcgcgc caagcaacag atcgacaagc gccccttcgg cacccagggt gacgtctacc
    18301 agacggacta tgaatggatc aaccattcca tgacgcccac gcaccccgcc aacagcgact
    18361 ttcgcatcac cgtgggcgga ccggactgta cccagccggt gtcactatcg gtgctgaaca
    18421 tctccgccat gagcttcggc gcgctgtcgg ccaacgccgt catggcgctg aacaaaggcg
    18481 cggcgaccgg gaacttcgcc cacgacacag gcgaaggcgg cgtcagccgc taccacctgg
    18541 cgcacaacgg cgcattgatc tggaacatcg ggtccgggta cttcggctgc cgcgacacgg
    18601 cgggcaattt ctcggaagag gaattcgtca aacaggccac ccgcgacaac atcaggatga
    18661 tagagatcaa gttgtcgcaa ggcgccaagc ccggccatgg cggcatcctc cccggcccca
    18721 aggtcacacc cgagatcgcc gccgcacgcg gcgtgccggc ctggcaggac tgcaactcgc
    18781 cctcggcgca cagtgcgttc gacaccccca tcggactcat gcatttcatc gcgcggctgc
    18841 ggaacctcag cggtgggaaa ccggtggggt tcaagctgtg cgtgggccac ccctgggaat
    18901 ggttcgccat cgccaaggcc atgctcgaaa ccggcatcac gccggacttc atcgtggtcg
    18961 atggcgccga gggcggcacc ggcgccgccc ccatcgaatt cgttgatcac gtgggcacgc
    19021 cgctgcgcga aggcctgcgc ctcgtacaca acacgctggt cggcatcggc ctgcgcgacc
    19081 gcatcaagct gggcgcatcc ggcaagatca tcagcgcctt cgacatggcg cgcatcatgg
    19141 cgttgggtgc cgactggtgc aacagcgccc gtgccttcat gttcgcggtc ggttgcctgc
    19201 aggcgcaggt ctgccatact gggcgctgcc cgacgggcgt caccacacag gatccgaagc
    19261 gtcagaaggc gctggtggtg gctgacaaat ccgtgcgcgt ggccaacttc cacaccaata
    19321 ccttgaaggc cctggccgag ctattgggcg cggcggggct gacgcacccc ggtgacttgc
    19381 gcccacacca catcgcgcga cgcatcgcca acggcgaaat ccgggtgttg tcggcactgt
    19441 tccccgatct ggaaaacggt gaattgctgg ccggaaaatt ccgcaacacg gtcttccgca
    19501 cggcctggcc catggcgcgt gccgagtcct tcgatccggc ctacagtctc agcgcggcgc
    19561 tatcgggcca cctggacatc agcatccccg aggacacgca tgcgggccaa gcgcgcgtca
    19621 actgacctcc aacccgaccc accagggccg gtcaccatgt catgcgggca gcccgaatca
    19681 ggcccgcctc cggtcggccc aggcctccac ggccgtcgac ggtgaccggg ggaaggcccg
    19741 ggccattgca catcattgcg ctggtgcggg gtatatcggc ttgccggttg cggcttcagc
    19801 cggatagaca accgagaact tcaacccgcc cttgccgtcc ggcaccaact ggccaagtgg
    19861 catgacctcg ccggtctggg cgccgtcctt gtccagttca aacgcgccat ccagcgtttt
    19921 gagcttgccg gacaggctga agacggcttt gcgcaggtcg agttgatcca gactgttggt
    19981 ggccgccagg gtgttctgga tcacgacacc ggtggtgtag cctgccaccg agttaaagcc
    20041 gaacgacacc ttgctgtctg ggtaggcctt gtcccaggct gtgtggaatt caggcagtgt
    20101 catgcctgac gtcaccttgt actcgacttc cgacggcggc gtgtaggtgt agacgtactt
    20161 catgccgtcc acgcccaccg tcttgagcat gtgctcggtt tccaaacccg ggaacacggc
    20221 gaacaaccat ttgaaatgtg cccccgaatc atgcaggttg cgcaggaacg ccgtatcgtt
    20281 gcccgggtag ccgagctgaa tcacggcgtc gggcttggcg gcccggatgc tgttgagcag
    20341 cacggtgtag ctcgaggtgt tcgtgggcac ccccgtgtcg tacacgatct tgaccggtgc
    20401 atgcgcgtct tcgatcagct tgcgcatggc cttggcctgc gtgccggaga aatcattcgt
    20461 ggcgtagatc agggcgatgt gcttgatgcc cagcttcgga ccttccgtcg tcaggaaatt
    20521 ggccacgctt ttcggccaga ccgtcgacac gggcatggcg accagggcga tgtaagggtt
    20581 gccgggtgtg aagaacgggt cgcccgtgcc gctcagatcg aacagcatca tcttgtgttc
    20641 gcgcgcgatg ggcacggcca tggaggtcag caccgacccg acgtcggcca ccagcacgtt
    20701 gaccttgtcc tgggtgatga gctggttgta caaggtggcg gccgtggagg tgttgctctg
    20761 atcgtcatag gcgacgagct tgacgggaat gcgtttgccg tagggcttga ccatcacacc
    20821 gcccttggcg ttgacctcgt ccacccacag cttcaacccc atgtgcgccg gcatggaaat
    20881 ggccgcgaac ggccccgatg acgcgtacaa cgcaccaatc ttgatttccg ccggcgcctg
    20941 gggggccggt gtggcggcgg ccagggtgga gaacaacaac aacgacgcgg ccgacagcca
    21001 cgaaaaaatt ctgcgcaaca tcatgagtct ccttgggttg tcgccggacc acgtcgcagt
    21061 ccggcatttc tggtccggca tttctattac ctgcctgcat cacgaatgaa ggatcaagaa
    21121 cgatc
//
LOCUS       DASTNK010000174         6542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1095486, whole genome shotgun sequence.
ACCESSION   DASTNK010000174 DASTNK010000000
VERSION     DASTNK010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095486"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="VFR20_12720"
     CDS             <1..1045
                     /locus_tag="VFR20_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEU0231424.1"
                     /translation="NQKYLTNPAELPDDLHWSFWKAYTTWLSGFGLFFIMYLLKPEFY
                     LVNANSPWQWAAHMSGVEASVLAVAFLVVGYIVYSQLCQRISPTMNRDGWLSAAVGVM
                     MIIVAWLTTMVFPGRAAFLITGALMATCMAANVFFWIIPGQRRMVTALKAGKTPDPLD
                     GKRGKQRSVHNTYFTLPVVFLMLSNHYAFTYTSAHAWIVMALFIFAGAVIRQYFVLRH
                     VGKNQLAYPAVGIVMLLIIGWLVMPAPAAPVAPSAPSAAHGAAVATATSVDTKQVRTI
                     VNARCIECHSVKPTQPGFASAPAGIMFDTNAELLLHEAQIKQVVASKYMPLGNMTKMT
                     DAERAAIAAWVAK"
     gene            1270..1557
                     /locus_tag="VFR20_12725"
     CDS             1270..1557
                     /locus_tag="VFR20_12725"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEU0231425.1"
                     /translation="MKMQLKLIGAPSALLLLEGWSSVESRDAIRKTFVFKNFSEAFGF
                     MTRVAMQAEKLDHHPEWSNVYNRVEVTLTTHDAKGVTERDVALALFMDQAA"
     gene            1868..3391
                     /locus_tag="VFR20_12730"
     CDS             1868..3391
                     /locus_tag="VFR20_12730"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEU0231426.1"
                     /translation="MTNLKNTVLLDSSGQLSPTILPPLIPYAGYRDARAAVERLVEIY
                     RRNTAFIRAAFADYAAGKLPAGQRVRAYYPAVRIKTHSYQEVDSRLSYGHVIEPGAYM
                     TTITQPDLYFDYLEEQIGLLIKNHNVPVEVGESDMPIPLHFAFPDGRYVEGISPDTLG
                     DALRDHFDVPSLAVTDDAIVNGTWQGRQGEPQPLSPFTAPRVDYSLHRLRHYTATEPG
                     YFQNFVLFTNYQFYVDEFCQWAREMLAQSEGGYTALVEPGNIITPRGADGERSGEPLS
                     RLPQMPTYHLMRENHSGITMINIGVGPSNAKTITDHVAVLRPSAWLMLGHCAGLRTTQ
                     RLGDYVLAHGYVRQDHVLDADLPTWVPVPPLAEIQVALEQAVAEVAGLSGWDLKRIMR
                     TGTVATIDNRNWELRDHVELVQRFSQSRAIALDMESGTIAANGFRFRVPYGTLLCVSD
                     KPLHGELKLPGMASEFYRTQVGQHLHIGIRALENLRDMPRERLHSRKLRSFVETAFK"
     gene            complement(4016..4813)
                     /locus_tag="VFR20_12735"
     CDS             complement(4016..4813)
                     /locus_tag="VFR20_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0231427.1"
                     /translation="MSEKKLAGRVALVFGAGSVGEGWGNGKAAAVSYAREGAAVIAVD
                     LNAAAAEATRDIILSEGGQCDAFAADVTQSEQIRALVAEIIGHHGQLDILHNNVGMAK
                     MGSVTDLSEADWDLAMDVNLKSAFLTCKHVLPIMQAQKRGAIINISSLAAIRYTGYPY
                     PVYYAAKGGLNQLTAGLALEYAGSGIRVNAIMPGYIDTPLIYKDISGQYASKEAMVAE
                     RNARCPMGHMGTAWDIARAAVFLASDDAAYITGVCLPVDGGVHMTSR"
     gene            complement(4881..>6542)
                     /locus_tag="VFR20_12740"
     CDS             complement(4881..>6542)
                     /locus_tag="VFR20_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEU0231428.1"
                     /translation="LWLAMLGAAVAFRRGEHMRMTALVNKASPATRALLDVFAVMVSL
                     VFVLLVLLPSFEATIVQSVIHTAAMGISRSWRTAALPTGLSFMAIFAILRLIEASNWK
                     LVLKALGLIVLIGLVLYALSPLFEDLGNYNLLIFFVGLVALGVFTGVPIAFSFGIATY
                     AYLLLSAQVPLTVLVGRMDEGMSHLILLAVPLFVFLGLLIEMTSMAHKMVSFLANLLG
                     HVRGGLSYVLVGAMYLVSGISGAKAADMAAIAPVLFPEMKKRGADEGELIALLSAAGA
                     QTETVPPSLVLITIGSVTSVSITALFKGGLLPSAVLGLMLCMVVWWRNRHQKLDHVER
                     QPKLEILKSFIIAFPALTLPFVIRAAVVEGVATATEVSTIGIVYSCFIGIFVYRCFDV
                     KRLYPMLIDTAILSGAILLIIGTATGMAWALTQSGFSSDLADYMGNLPGGAFTFFCVS
                     IVTFIVLGSVLEGIPAIVLLGPLLFPIAAELNINEVHYSMVAVLAMGIGLFAPPFGVG
                     YYAACAIGRVAPDKGIKPIIGYLCSITIGLIIIAAVPWLSTGFLK"
BASE COUNT         1192 a         2084 c         2130 g         1136 t
ORIGIN      
        1 caaccagaag tacctgacca accccgccga actgcccgac gacctgcact ggtcgttctg
       61 gaaggcctac accacctggc tgtccgggtt cggcctgttc ttcatcatgt acctgctcaa
      121 gccggagttc tatctggtca atgcaaacag cccttggcag tgggccgcgc acatgagcgg
      181 tgtcgaagcc agtgtgctgg cggtggcgtt cctggtggtc gggtacattg tttattcgca
      241 gctttgccag cgcatcagcc ccaccatgaa ccgcgatggc tggctcagcg cggccgtggg
      301 cgtcatgatg atcatcgtgg cctggctcac caccatggtc ttcccggggc gcgccgcgtt
      361 cctcatcacc ggggcgctca tggccacgtg catggccgcc aacgtgttct tctggatcat
      421 tcccggccag cgccgcatgg tcacggcgct caaggccggc aagacgcccg atccgctgga
      481 tggcaagcgc ggcaagcagc gctcggtgca caacacctat ttcacgctgc cggtggtgtt
      541 cctgatgctg agcaaccact acgcgttcac gtacaccagc gcgcatgcct ggatcgtgat
      601 ggcgctgttc atcttcgccg gtgccgtgat ccggcagtat ttcgtgttgc gccatgtggg
      661 aaagaatcag ttggcctacc cggcggtggg catcgtcatg ctgctgatca tcggctggct
      721 ggtcatgccg gcgccagccg cgccggtggc gccttccgca ccctccgccg cacacggcgc
      781 cgcggtggcc acggccacct cggtcgatac gaagcaggtg cgcaccatcg tgaacgcgcg
      841 ctgcatagag tgccattcgg tcaagcccac acagccgggc ttcgcgtcgg caccggccgg
      901 gatcatgttc gacaccaacg ccgaactgct cctgcacgaa gcgcagatca aacaggtcgt
      961 ggccagcaag tacatgccgc tgggcaacat gaccaagatg acggacgcgg aacgcgcggc
     1021 catcgccgcc tgggttgcca agtagccgtt gctccagcgg tgttctcatg ccgcgccggc
     1081 ccccgcctcg gagccggcgc ggcatggtgt gtcggcttgc acgcgcggga aagcgcctcc
     1141 cgcgttgccg ggcctcgccg gtgatgaatg gccagggcgg tcagtgaacg gaaaaatcct
     1201 cctgaaacct gccagtcttc tcacggacga gtagactgtg ggtatacatg ttgcccccag
     1261 gaggtgccca tgaagatgca gctcaagttg atcggggcgc cgtcggcgct gttgctgctc
     1321 gaaggttggt catcagtgga atcgcgcgat gccatccgca aaacattcgt gttcaagaat
     1381 ttcagcgaag cgttcggttt catgacgcgc gtggccatgc aggccgaaaa gctcgaccat
     1441 catcccgaat ggtccaacgt ctacaaccgc gtcgaagtca ctctgacgac gcacgacgcc
     1501 aagggcgtga ccgagcgtga cgtggcgctg gccctgttca tggaccaggc cgcctgagcg
     1561 gatgccgaca gtggcccgcc agaccgccat gcctgattcg acaactgctc gctcgcccta
     1621 ttcgtccgcg gtggcgttgc ggccccgcgc ggtggccacg gttgtggcgc gtcgccatct
     1681 attgccgcaa gcgccgatgg ctgtgctcca ttactccgaa ttcaagtaga attttttgtt
     1741 gaccgtgcgc tgtggcgcgg gggcggtaaa gacgtccacg caaacctggc cagcgacgaa
     1801 agaggcgttg tgtctctttc cacggggtgg ggccggacga ccctgcgttc cgacgtaaca
     1861 tcgaggcatg accaatttga aaaacaccgt cttgcttgac tcttcggggc agctcagccc
     1921 cacgatcctg cccccgctta ttccgtacgc cgggtatcgg gacgcccgcg ccgccgtcga
     1981 gcggctggtt gaaatctacc ggcgcaacac ggcgttcatc cgcgccgcct tcgcggacta
     2041 tgccgccgga aagctgccgg caggccagcg ggtacgggcc tactaccccg ccgtgcgcat
     2101 caagacgcac tcgtaccaag aggtcgacag ccgtctgtca tacggccatg tgatcgagcc
     2161 gggcgcctac atgacgacca tcacacagcc ggatctgtac ttcgattatc ttgaagagca
     2221 gatcgggctg ctcatcaaga accacaacgt gccggtggag gtgggtgaat cggacatgcc
     2281 cattccgctg catttcgcgt ttccggatgg gcgctacgtg gaagggattt cacccgacac
     2341 actgggtgat gccctgcgcg accatttcga cgtgcccagc ctggccgtca ccgacgacgc
     2401 catcgtcaac ggcacctggc aggggcgtca gggcgagcca cagccgctgt cgccgttcac
     2461 cgcgccgcgc gtcgattatt ccctgcatcg gttgcggcac tacaccgcca ccgagcccgg
     2521 gtatttccag aatttcgtgc tgttcaccaa ctatcagttt tacgtcgacg agttctgcca
     2581 gtgggcacgc gagatgctcg cgcagagcga aggcggctac acggcccttg tcgagcccgg
     2641 caacatcatc acgccgcgcg gcgcggacgg cgaacgcagt ggcgagccgc tgtcgcgtct
     2701 accgcagatg cccacgtatc acctgatgcg tgagaaccat tccggcatca ccatgatcaa
     2761 catcggcgtt ggcccgtcca acgccaagac catcaccgat cacgtcgcgg tgctgcggcc
     2821 gtcggcatgg ctgatgctgg gccattgcgc cggcctgcgc acgacgcagc ggctgggtga
     2881 ttatgttctg gcgcatggct atgtacgcca ggaccacgtc ctggacgcgg atctccccac
     2941 ctgggtgccg gttccgccgc tggccgagat ccaggtggcg ctggagcagg ccgtggcaga
     3001 ggtggccggc ctgtcggggt gggatctgaa gcggatcatg cgcaccggca cggtggccac
     3061 catcgacaac cgcaactggg aactgcgcga ccacgtcgaa ctggtccagc ggttctcgca
     3121 gtcgcgcgcc attgcgctgg acatggaatc gggcacgatc gccgccaacg ggttccgttt
     3181 ccgcgtgccg tatggcacct tgctgtgcgt gtccgataag ccgctgcacg gggaattgaa
     3241 gctgccgggc atggccagcg agttctatcg cacccaggtg gggcagcatc tgcacatcgg
     3301 catccgggcg ctggagaacc tgcgcgacat gccgcgtgag cgtctgcatt cccgcaagtt
     3361 gcgcagtttc gtggagacgg cgttcaagta ggtcaccggc gtggggtttt gctgggttcg
     3421 acgttgtagg tatctgaaga cgctgtctat cggggccagg ggcgtgcccg gcacggcgtg
     3481 cttcatccgg atctggcttg ggaaggcggg cgtcgggtgg aactcgcaaa atggtcagtc
     3541 ctacggtctg accatttcgc tcagacagcc ccccgacgac tgcccccgcc ttcccaagcc
     3601 agatccggcg cacgccttac accgggccca cccctggcct cgacagccag ctcgcgatga
     3661 gggcatgcgg gggagtcggc aaaccgccca caactgcggc agtatctctc catgcatgcc
     3721 gcaatcgtag ctccagggtg ggagaagtag cgcaggccgg cgtttcggcc agcgccggat
     3781 ctggcttggc caggcggggg cagtcgtcgg gcggtgtttg aacggagcct cgctatcgcg
     3841 aggcgtagtg agttcagccc gacgcccgcc tggacgagac agacccgggc agtcggggcg
     3901 cgcagtgcca cgaccgctgg acgcgccggc ctgcgctact tctcccaccc tggagcgtgt
     3961 caagcaaaaa cccgcttcgg ccaaaccggt ttaaacgatc aagcgcaatg cgtgttcacc
     4021 gcgacgtcat gtgcacaccg ccgtccaccg gcagacagac ccccgtgatg taggccgcgt
     4081 cgtccgacgc caggaatacc gcggcgcggg cgatgtccca ggcggtgccc atgtggccca
     4141 tggggcagcg cgcgttgcgc tcggccacca tggcttcctt gctggcgtac tggccggaaa
     4201 tgtctttgta gatgagaggc gtgtcgatgt agccgggcat gatggcgttg acgcgtatgc
     4261 ccgaccccgc gtattccagg gccagccccg ccgtgagctg attcaacccg cccttggccg
     4321 cgtaatacac cgggtagggg tacccggtgt agcgaatggc cgccaggctt gagatattga
     4381 tgatggcgcc gcgcttttgc gcctgcatga tgggcagcac gtgcttgcac gtgaggaacg
     4441 cgctcttcag gttcacgtcc atggcgaggt cccagtcggc ctcggacaga tcggtgacac
     4501 tgcccatctt cgccatgccc acattgttgt gcaggatgtc gagctgcccg tggtggccga
     4561 tgatctcggc caccagggcc cgtatctgct ccgactgggt gacgtcggcc gcgaaggcat
     4621 cgcactgtcc gccttccgac agaatgatgt cgcgggttgc ctcggctgcg gcggcattca
     4681 ggtcgacggc gatcacggcg gcgccttcgc gcgcatacga caccgccgct gccttgccat
     4741 tgccccagcc ttcgccgacg gagcccgcac cgaaaaccag cgccacgcgc ccggccagtt
     4801 ttttctcgct catgtggatt cgatccatga aaaatggaga gcggccgcgc ccgcccgttt
     4861 cgtggggcgg gcggcggcca ttacttcaga aagcccgtgg acagccaggg cacggcggcg
     4921 atgatgatca ggccgatcgt gatggagcac aggtagccga tgatgggctt gatgcccttg
     4981 tcgggcgcga cgcggccgat ggcgcaggcc gcgtaatagc ccacgccgaa tggcggcgcg
     5041 aacaggccga tgcccatggc cagcactgcg accatggaat agtgcacttc gttgatgttc
     5101 agctccgccg caatggggaa cagcagcggg ccgagcagca cgatggccgg gatgccttcg
     5161 agcacactgc ccagcacgat gaacgtgacg atggacacgc agaagaaggt gaacgcgccg
     5221 ccaggcaggt tgcccatgta atcggccagg tcgctggaga accccgattg cgtcaatgcc
     5281 caggccatgc cggtggccgt gccgatgatg agcaggatgg cgcccgacag gatggccgtg
     5341 tcgatgagca tggggtacag gcgcttcacg tcgaagcatc ggtacacgaa gatgccgatg
     5401 aagcacgaat acacgatgcc gatggtggag acctcggtgg ccgtggccac cccctcgacg
     5461 accgccgcgc ggatgacgaa aggcagcgtc agcgccggga aggcgatgat gaaactcttg
     5521 aggatttcca gcttcggttg gcgttcgacg tggtcgagct tctggtggcg gttgcgccac
     5581 cagaccacca tgcagagcat gaggcccagc accgcgctgg gtagcaggcc gcccttgaac
     5641 aacgcggtga tggacacgct ggtgaccgaa ccgatggtga tgagcaccag gctgggcggc
     5701 acggtttctg tttgggcgcc cgcggccgag agcagcgcga tgagctcgcc ctcgtcggca
     5761 ccgcgttttt tcatttctgg gaacagcacc ggcgcgatgg ccgccatgtc ggccgccttg
     5821 gcgcccgaaa tgcccgacac caggtacatg gcgccgacca gcacgtacga caggccgccg
     5881 cgcacgtggc cgagcaggtt ggccaggaac gacaccatct tgtgcgccat gctggtcatt
     5941 tcgatgagca ggcccaggaa gacgaacagg ggcacggcca gcaggatgag gtgcgacatg
     6001 ccttcgtcca tgcggccgac caggacggtg agcggaacct gggcgctgag gagcaggtag
     6061 gcgtaggtgg caatgccaaa cgaaaaggcg atgggaaccc cggtgaacac gcccagcgcc
     6121 accaggccca cgaagaagat gagcaggttg tagttgccca ggtcttcgaa gagcggggac
     6181 aaggcataca gtacgaggcc gatgaggacg atcaggccca gcgccttgag gaccagcttc
     6241 cagttcgatg cctcgatgag ccgcaggatg gcgaagattg ccatgaacga caggcccgtg
     6301 ggcagcgcgg cggtacgcca ggaacggctg atgcccatgg ccgccgtatg gatgacggac
     6361 tggacgatgg tggcttcgaa gctgggcagc agcaccagca gcacgaacac cagcgacacc
     6421 atgacggcga agacatcgag gagcgcccgc gtggccggcg aggccttgtt gaccagggcc
     6481 gtcatgcgca tgtgctcgcc gcgccggaac gccacggcgg cgcccagcat ggcgagccac
     6541 ag
//
LOCUS       DASTNK010000175         4246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1467136, whole genome shotgun sequence.
ACCESSION   DASTNK010000175 DASTNK010000000
VERSION     DASTNK010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467136"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VFR20_12745"
     CDS             <1..1135
                     /locus_tag="VFR20_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU0231429.1"
                     /translation="LKPTSAALILAGITLAFGQTASADVVVKIGSAAPLTGNIAHIGQ
                     DQADGAQLAVDQINKSGKLVINGQKIKLVLDSQDDQADPKTGTQVAQKLVDDGVVAVV
                     GDLNSGVTIPASKIYSDNGVTQISPASTNPQYTLQGYKTAFRVIGTDAQQGPALAKYA
                     KKLGFKRVAVVDDATAYGQGLADQFEKTAKAEGIKVLSRDATNDKATDFKAILTKIKA
                     ERPDVIFYGGMDATGGPFSKQARQLAIRARILAGDGVCTTDLSKLAGNATDNVICSIA
                     GGDLSKMAGGAQFAKDMKATFGHDPVIYSPTSYDAVGVIVDAMKRSKSITRAGILSAM
                     PSTNYNGLTGNIQFDQHGDLKGASISLYKYVNGKQTLLDVIKM"
     gene            1322..2272
                     /locus_tag="VFR20_12750"
     CDS             1322..2272
                     /locus_tag="VFR20_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0231430.1"
                     /translation="MDIFVQQVINGLILGSMYALIALGYTMVYGILGIINFAHGDILM
                     VGAMVAMTTVNFLQHHFPNIGNIPLVLIALVVACIVCAIVGFTIERWAYRPLRNAPRL
                     APLLTAIGISITLETLAMVIWSPDPLPFPQLLPTDPINVIPQTATTIGVVISGTEITI
                     IATAFLVMAGLLLLVHKTKLGRAMRAIAESPSNASLMGVNPNFVISATFMIGSALAAL
                     AGLMMALEYGHVHFYMGFIPGLKAFTAAVLGGIGNLGGAMVGGIVLGLIEQLGAGYIG
                     NLTHGIFGSNYQDVFAFIVLILVLVFRPSGLMGERVADRA"
     gene            2286..3407
                     /locus_tag="VFR20_12755"
     CDS             2286..3407
                     /locus_tag="VFR20_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0231431.1"
                     /translation="MNSNKSKTVLHIAFAILVLIAPVLIDTVGGNYWVRVLDFAMLYV
                     MLALGLNVVVGFTGLLDLGYIAFYAVGAYTAALLSSPHLTSHFAWIAHLFPAGLHTPY
                     LIIAVIGMALAAIFGILLGAPTLRLRGDYLAIVTLGFGEIVRIFMNNLYSPVNITNGP
                     QGINNISPVHFEGFNLADTHTFFGIHFTSSIQYYYLFLIGSLIVIAVCTRLQGSRIGR
                     AWAAIREDELAAKAMGINTRNMKLLAFAMGASFGGLAGSMFGAFQGFVSPESFTFWES
                     TVVLACVVLGGLGHIPGVILGAILMSVFPELLRDTVGPLQQAVFGHQIISPSVIRQGL
                     YGLAMVFIMLYRPAGLWPAPTHENRIAKLNKRNRKQATA"
     gene            3438..4211
                     /locus_tag="VFR20_12760"
     CDS             3438..4211
                     /locus_tag="VFR20_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009911514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0231432.1"
                     /translation="MSQDIRLSIKGVNKRFGGLQALSDVGLQIRAGEIYGLIGPNGAG
                     KTTFFNVITGLYTPDSGEFMLEGKPYKPTAVHEVTQAGIARTFQNIRLFGGMTALENV
                     MVGRHVRTHQTILGAVFRTRAERREEQDIRERARELLDYVGISQYADYTSRNLSYGHQ
                     RRLEIARALATDPKLLALDEPAAGMNATEKVDLTSLLDKIRADGRTILLIEHDVKLVM
                     GLCDHMTVLDYGKIIAEGLPADVRKNPKVIEAYLGTGGH"
BASE COUNT          821 a         1482 c         1170 g          773 t
ORIGIN      
        1 gctcaaaccc acaagcgccg cgctgatcct cgccggtatc accctggcgt tcggccaaac
       61 cgcttccgcc gacgttgtcg tcaagatcgg cagcgccgcg ccgctgaccg gcaacatcgc
      121 ccacatcggc caggaccagg ctgacggcgc ccaactcgcc gtcgaccaaa tcaacaagtc
      181 cggcaagctg gtcatcaacg gccagaagat caagctggtg ctcgattcgc aagacgatca
      241 ggccgacccc aagaccggca cgcaagtcgc ccagaagctg gtcgacgacg gcgtcgtggc
      301 cgtggtgggc gacctgaact ccggcgtcac cattcccgca tccaagatct acagcgacaa
      361 cggcgtgacc cagatttcac cggcctcgac caacccgcaa tacacgttgc aaggctacaa
      421 gaccgccttc cgcgtgatcg gcaccgatgc ccagcaaggt ccggccctgg ccaaatatgc
      481 caagaagctg ggtttcaagc gggtcgccgt ggttgacgac gccaccgcct atggccaagg
      541 cctggccgac cagttcgaga aaaccgccaa ggctgagggc atcaaggtgt tgtcgcgcga
      601 cgccaccaac gacaaggcca ccgacttcaa ggccattctg accaagatca aggccgagcg
      661 ccccgacgtc atcttctacg gcggcatgga cgccaccggc ggcccgttct ccaagcaggc
      721 ccggcaactg gccatccgcg cccgcatcct ggccggcgac ggcgtctgca ccaccgacct
      781 gagcaagctg gccggcaatg ccaccgacaa cgtgatctgc tcgatcgcag gcggcgatct
      841 gtccaagatg gctggcggcg cccagttcgc caaagacatg aaggccacgt tcgggcacga
      901 tccggtgatc tattcgccaa cctcgtacga cgccgtcggc gtcatcgtcg acgccatgaa
      961 acgctccaag tcgatcacgc gcgccggcat cctgtcggcc atgccctcga ccaactacaa
     1021 cggtctgacc ggcaatatcc agttcgacca gcacggcgac ctgaagggcg cctcgatctc
     1081 tctctacaag tacgtcaacg gcaagcagac cctgctcgac gtcatcaaga tgtaatccgg
     1141 gccgggatcc cgcgtgaacc cctctggttt ccgcgggatc ccccattggc cacttccagc
     1201 gccatcccaa gacccgccct cttctccaag ccggtgcttc ttctatcccc ccgatactcg
     1261 agccctcccg cgcgctcata aggcgactgc tggcgggcgc gaaagacaca tctgaatcgt
     1321 catggatata ttcgttcaac aagtcatcaa tggcctgatc ctgggcagca tgtacgccct
     1381 catcgccctg ggctacacga tggtgtacgg catcctcggc atcatcaact tcgcgcacgg
     1441 cgacattctc atggtcggcg ccatggtcgc catgacgacg gtcaatttcc tgcaacacca
     1501 cttcccgaac ataggcaata tccctctcgt gctgatcgcc ctggtggtgg cgtgcatagt
     1561 ctgcgccatc gtcggcttca cgatagagcg ctgggcctac aggccgctgc gcaacgcgcc
     1621 acgtctggcg ccgctgctca ccgccatcgg catatccatc acgctcgaaa cccttgccat
     1681 ggtcatctgg tcgcccgatc cgctgccctt cccgcagctc ctgcccaccg acccgatcaa
     1741 cgtcatcccc cagaccgcca ccacgatcgg cgtggtgatc tccgggaccg agatcaccat
     1801 catcgcaacg gcttttctcg tcatggcggg cttgctgctc ctggtgcaca agaccaagct
     1861 tgggcgcgcg atgcgcgcca tcgccgagag ccccagcaac gccagcctga tgggcgtgaa
     1921 ccccaacttc gtgatctcgg ccaccttcat gatcggttcg gccctggccg ccctggccgg
     1981 cctgatgatg gccctggaat acggccacgt acacttctac atgggtttca ttcccggcct
     2041 gaaagccttc accgccgcgg tactgggtgg catcggcaac ctgggtggcg ccatggtggg
     2101 cggcatcgtt ctggggctga tcgaacagtt gggcgcgggc tacatcggca atctcacgca
     2161 cggcatattc ggcagcaact accaagacgt cttcgcattc atcgtgttga tcctcgtgct
     2221 ggtcttcagg ccctccggcc tgatgggcga acgcgtggcc gatcgcgctt aatgggggcc
     2281 acgacatgaa ttccaacaaa agcaaaaccg tattgcacat cgccttcgcg atactggtcc
     2341 tgatcgcccc cgtgctcatc gacacggtgg gtggcaacta ttgggtgcgt gtgcttgact
     2401 tcgccatgct ctacgtcatg ctggcgctcg gcctgaacgt ggtggtgggc ttcaccggcc
     2461 tgctcgacct gggctatatc gccttctatg ccgtaggcgc ctacacggcg gcgttgttgt
     2521 cgtcgccgca cctgaccagc cacttcgcat ggatcgccca cctgttcccg gccggcctgc
     2581 acacgcccta tctcatcatc gcggtgatcg gaatggcgct ggccgcgatc ttcggcatat
     2641 tgctgggcgc gcccacgctg cgcctgcgtg gcgactacct ggcgatcgtg accttgggct
     2701 tcggggagat cgtgcgcatc ttcatgaaca acctgtacag cccggtcaac atcaccaacg
     2761 gcccccaggg catcaacaac atttcgccgg tgcacttcga gggcttcaac cttgccgaca
     2821 cacacacatt cttcggcatc cacttcacgt catccattca gtactactac ctgttcctga
     2881 tcggatcact gatcgtgatc gccgtgtgta cccgccttca aggctcacgc atcggccgcg
     2941 cctgggcggc catccgggag gacgaactgg ccgccaaggc catgggcatc aacacccgca
     3001 acatgaaact gctggccttc gccatgggcg cgtcctttgg cggcttggcg ggttccatgt
     3061 tcggtgcctt ccagggcttc gtttccccgg aatccttcac cttctgggaa tccaccgtcg
     3121 tgctggcctg tgtggtgctg ggcggcctgg gccacattcc gggcgtgatc ctgggggcca
     3181 tcctgatgtc ggtcttcccc gagttgctgc gcgacacggt gggccctctt cagcaagccg
     3241 tgttcggcca tcagatcatc agcccctccg tcatccggca gggcctctac ggcctggcca
     3301 tggtgttcat catgctgtac cgtccggcgg gcctatggcc agcgcccacg catgagaacc
     3361 gcatcgccaa actcaacaag cggaatcgca aacaagcgac cgcataaggc caagatccac
     3421 agattacgcg aatcagcatg agccaagaca ttcgactttc aatcaagggc gtcaacaagc
     3481 gcttcggcgg cctgcaggcg ctctccgacg tgggcctgca aatccgcgcc ggcgaaatct
     3541 acggcctgat cggccccaat ggcgccggca agaccacttt cttcaacgtc atcaccggcc
     3601 tctacacgcc ggactcgggc gagttcatgc tcgagggcaa gccctacaaa cccacggccg
     3661 tgcacgaagt cacccaggcc ggtatcgccc gcaccttcca gaacatccgc ctgttcggcg
     3721 gcatgaccgc cctggagaac gtcatggtgg ggcgccacgt gcgcacgcac cagaccattc
     3781 tgggcgcggt cttccgcacc cgggccgaac gccgagagga acaagacatc cgcgagcgcg
     3841 ccagggagct gctcgactac gtgggtatct cgcaatacgc cgactacaca tcgcgcaatc
     3901 tctcgtacgg ccaccaacgc cgtctggaga tcgcccgcgc gttggccacc gaccccaaac
     3961 tgctggcgct cgacgagccc gccgccggca tgaacgccac cgagaaagtg gatctcacct
     4021 ccctgctcga caagattcgc gcggacggcc gcaccattct gctgatcgag cacgacgtca
     4081 agctggtcat gggcctgtgc gaccacatga ccgtgctgga ttacggcaaa atcatcgctg
     4141 aaggactacc cgcggatgtc cggaagaatc cgaaagtcat cgaagcctat ctgggcacgg
     4201 gaggtcacta agccatgtcc gaaacgctac tcaaaatcac ggggct
//
LOCUS       DASTNK010000176         4116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_172360, whole genome shotgun sequence.
ACCESSION   DASTNK010000176 DASTNK010000000
VERSION     DASTNK010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172360"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VFR20_12765"
     CDS             <1..187
                     /locus_tag="VFR20_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU0231433.1"
                     /translation="HVPLSPHVVRHAFATHLLNHGADLRVVQMLLGHADISTTQIYTH
                     VARERLKPLHAQHHPRA"
     gene            218..712
                     /locus_tag="VFR20_12770"
     CDS             218..712
                     /locus_tag="VFR20_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEU0231434.1"
                     /translation="MSKQKHISETPATQFLKRNKVAFGEHPYEYVDHGGALEAARQLD
                     LDPHCVAKTLIMEDEHSHPLVILMHGDRDVSTKNLARQIGVKKVAPCHPEVAQKHSGY
                     QVGGTSPFATRRKMPVWVQAGLLDYPLIYLNGGRRGYLISIAPAVVTDLLGAKRVDVG
                     LDKE"
     gene            complement(719..1537)
                     /locus_tag="VFR20_12775"
     CDS             complement(719..1537)
                     /locus_tag="VFR20_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEU0231435.1"
                     /translation="MKILISADIEGVAGVFHTDQTRPGNAEYERARLWMTQEANAAAL
                     GAFDGGATTVVINDSHGPFRNLLPDHLDDRIRCIQGKPRILGMMAGVESGCDAAFLVG
                     FHSRAQGRGVLAHTISSFAFSRVVLNGKELGEAGLYGALAGEYGVPVVFGTGDDRFIE
                     ENTPVLTSASWFQTKEASGRLSGCSLTPAASAAGIREAARQAMGRIAQARPFVIAPPI
                     QCDLTTTSGAMADLFSQLPSVERVDGVTIRFNTESVEAAVRTLNCFSAMSALLK"
     gene            complement(1534..2667)
                     /locus_tag="VFR20_12780"
     CDS             complement(1534..2667)
                     /locus_tag="VFR20_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEU0231436.1"
                     /translation="MSDSSLLPPVSSPIPACADSSGTCAPPRVGTLEVGSRNLISDVA
                     GVSVGHATLDQGSVQTGVTVVRPHPGDVFRDKVPAAVTVLNGFAKSIGLEQVRELGVL
                     ETPLGLTNTFSVPAVAQAQIRQAIAVHPEIGRALPTVNALVFECNDGYLNDIQAMAVE
                     ARHYQSAHDTADAVFQQGSVGAGRGMSCFQLKGGIGSASRLTPEDAAGVAHTVGVLVL
                     ANFGKLPMLTIAGHPVGRALAQPEASIDNEPEKGSIILIVATDAPLDARQLRRLSLRA
                     GAGLARTGSVFGHGSGDVALAFSTAYTVPSDTTRAMPDVRMLHDAALDPLFLACAEAA
                     EQAVVNALWHAQTVIGRDGNVRHGVRGHIPDWTHWLTQQGSAA"
     gene            complement(2660..3493)
                     /gene="gsiD"
                     /locus_tag="VFR20_12785"
     CDS             complement(2660..3493)
                     /gene="gsiD"
                     /locus_tag="VFR20_12785"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HEU0231437.1"
                     /translation="MSECWRKFRKQKLAMAAGVFLLILIVLAIIAPWIVPYNAENYFD
                     YDYLNAGPSALHWLGVDSLGRDIFSRILMGSRISLSAGFLSVALGGVAGTFLGLMAGY
                     YEGWWERITMRVSDVLLAFPGILLAIGVVAALGSSMVNVIVAVAVFSVPAFARLVRGN
                     TLSLKQMTYVEAARSVGASDWTLIFRHILPGTISPIVVYGTMRVGTSIITAASLSFLG
                     LGAQPPTPEWGAMLNEARADMVLAPHVAIFPALAIFLTVLAFNLLGDGLRDALDPKLD
                     V"
     gene            complement(3583..>4116)
                     /locus_tag="VFR20_12790"
     CDS             complement(3583..>4116)
                     /locus_tag="VFR20_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0231438.1"
                     /translation="RLCMTLAVSGISFPAFALGMMLIQIFSVQLGWLPTVGASTPAHY
                     ILPSITLGAAVASVMARFTRASFIEVMQEDYVRTARAKGVSETLVVIKHTLRNALIPV
                     VTMMGLQFGFLLGGSIVVEVVFSWPGMGSLLIDAVTMRDYPVIQGLVLLFSFEFILIN
                     LVVDVLYGVINPTIRYK"
BASE COUNT          846 a         1355 c         1256 g          659 t
ORIGIN      
        1 acacgtgccg ctctcacccc acgtggtgcg gcacgctttc gccacgcatc tgctcaacca
       61 tggcgccgat ctgcgggtgg tgcagatgtt gctgggccat gctgatatct cgaccacgca
      121 gatatacacc catgtcgccc gtgaacgcct gaagcccctg cacgcgcaac accatccccg
      181 ggcttgagcc gtagtccttt tccccctatc aacgcgcatg agcaaacaga agcacatctc
      241 ggaaaccccc gccacacaat tcctgaaacg gaacaaggtc gcgttcggcg agcatcccta
      301 tgaatacgtc gaccacggtg gcgcgctgga ggccgcgcgt caactggacc tggaccccca
      361 ttgcgtggcc aagacgctga tcatggagga cgagcattcg catccgcttg tcatcctcat
      421 gcatggggac cgcgatgtgt caacgaagaa cctggcaagg cagatcgggg tgaagaaagt
      481 cgctccctgt caccccgagg ttgcccaaaa gcactccggc taccaggtcg gagggacatc
      541 accgttcgcc acacggcgca aaatgccggt ctgggtgcag gccggtttgt tggattaccc
      601 actcatttat ctgaatggtg ggcgcagagg ctacctgatc agcatcgcgc ctgcggtggt
      661 gaccgacctg ctgggagcga agcgtgtcga tgtggggttg gacaaggagt gatccaactt
      721 acttcagcag cgccgacatc gccgagaagc agttcagcgt cctgacggcc gcttccacgg
      781 attcggtgtt gaaccggatc gtgacgccat ccacccgctc gactgaaggc aattgggaaa
      841 acaggtctgc catggcgccc gatgtggtgg tcaggtcaca ttgtatgggg ggcgcgatca
      901 caaacggccg ggcctgggcg atgcgcccca tggcttgccg ggcggcctct ctgatacccg
      961 cggccgacgc ggccggcgtc aatgaacacc cgctcaggcg accggacgcc tccttggtct
     1021 ggaaccagga agcggacgtc aacacaggtg tattttcctc gatgaaccgg tcatcgcccg
     1081 tgccgaacac cacgggcacc ccgtactcgc cagccagcgc gccgtacagg cccgcttcgc
     1141 ccaactcctt gccattgagc acgacgcggg aaaaagcgaa gctgctgatg gtatgggcca
     1201 gcacgccgcg cccctgcgcc cgcgaatgaa accccaccaa aaatgcggca tcgcatcccg
     1261 actccacacc cgccatcatg cccaggatgc gcggtttgcc ttgaatgcaa cggatccggt
     1321 cgtcgaggtg gtccggcagg agattgcgga acgggccgtg tgaatcgttg atcacaacgg
     1381 tggtggcgcc cccgtcgaac gcccccagtg ccgcggcgtt ggcctcctga gtcatccata
     1441 gccgggctcg ctcgtattcg gcattcccag gccgtgtttg atcggtgtga aagactcccg
     1501 ccacgccttc gatgtccgcc gagatcagaa ttttcatgcc gcgcttcctt gttgagtcaa
     1561 ccaatgcgtc caatcaggga tgtggccgcg aacgccatga cgcacgttcc cgtcccgtcc
     1621 gatgaccgtc tgtgcgtgcc acaacgcgtt gaccaccgcc tgctccgccg cttcggcaca
     1681 cgccaggaac agcggatcga gcgcagcgtc atgcagcatg cgcacatccg gcatggcgcg
     1741 cgtggtatcg ctgggcacgg tataggcggt ggaaaaggcc agcgccacat ctccgctgcc
     1801 atggccgaaa accgatccgg tgcgcgccaa tccagccccg gcgcgcagag acagacggcg
     1861 aagctggcgc gcatcgagcg gtgcgtccgt ggccacgatc aggatgatgg agcctttctc
     1921 cggttcattg tcgatcgagg cctcaggttg cgccaacgcg cgcccgaccg gatggcccgc
     1981 aatcgtaagc atcggcagct tgccgaaatt agcgagcacc aggaccccca cggtatgcgc
     2041 aacgcccgcg gcatcttccg gggtgaggcg cgaagccgac ccgatgccgc ccttcaactg
     2101 aaagcatgac atgccgcggc cggcgcccac actaccctgt tggaacaccg catcggccgt
     2161 gtcatgcgcc gactggtagt gccgcgcctc caccgccatg gcctggatgt cgttgaggta
     2221 cccatcgttg cattcaaaga ccagggcgtt gacggtgggt agcgcccgac cgatctcggg
     2281 atgcacggca atggcctgac ggatctgagc ctgggcgaca gccggcacgc tgaaggtgtt
     2341 ggtcaggccc agcggcgtct cgaggacgcc cagctcgcgc acctgctcca gacctatgct
     2401 cttggcgaaa ccgttgagca ctgtcacggc ggcgggcacc ttgtcccgaa acacatcccc
     2461 gggatgaggg cgaacgacgg tcacgccggt ctgcacgctc ccctgatcca gagtggcatg
     2521 ccccaccgat acgcccgcga cgtcggagat gagattgcgt gaacccacct ccagcgtacc
     2581 gacacgcggg ggggcacacg tcccggagga atcggcgcac gccgggatcg gggacgaaac
     2641 gggaggcaac aatgatgaat cagacatcga gttttggatc cagcgcgtcg cgcaagccgt
     2701 cgcccagcag gttgaacgcc agcacagtga ggaagatggc cagggccggg aaaatcgcca
     2761 cgtgcggtgc cagcaccatg tcggcgcgcg cctcgttcag catggcgccc cattccgggg
     2821 tgggcggttg cgccccaagg cccaggaacg acagactggc ggccgtgatg atggaggtgc
     2881 cgacgcgcat cgtgccgtag accacgatcg gcgagatcgt gcctggcagg atgtggcgga
     2941 agatcagggt ccaatccgac gcgcccacgc tgcgcgcggc ttcgacgtag gtcatctgct
     3001 tgagcgacaa ggtgttgccg cgcaccaggc gggcaaaagc gggaacgctg aacacggcca
     3061 cagccacgat cacattcacc atgctggaac ccagtgccgc caccacgcca atggccagca
     3121 gaatccccgg gaaagccagc aatacatcgg acacgcgcat ggtgatccgc tcccaccagc
     3181 cctcgtaata gcccgccatc aaccccagaa acgtgcccgc cacgccaccc aacgccacgg
     3241 agagaaaccc cgccgacagg gagatgcgcg accccatgag aatacggctg aaaatgtcac
     3301 ggcccaaaga gtcgactccc agccaatgca gcgccgacgg ccccgcgttc aggtagtcgt
     3361 agtcgaaata gttctcggcg ttgtagggca cgatccacgg cgcgatgatg gccagcacga
     3421 tcaggatcag caggaaaacc cccgcggcca tggccagttt ctgcttgcgg aatttgcgcc
     3481 agcattcgga caacggtgtc ctgacggcgc cgggtgagtt ggcgacgggg ggctgcgccc
     3541 ccggtggcgc ggtttgcgtg gcggtcgtat tcacctgcgg cctcatttat agcgaatggt
     3601 cgggttgatc acgccgtaca gcacgtcaac gaccagattg atcagaatga actcgaacga
     3661 gaacaacagc accaacccct gtatcacagg gtaatcacgc atggtgaccg cgtcgatgag
     3721 gaggctgccc atgccgggcc aactgaacac gacctccacc acgatggacc cgcccagcag
     3781 gaacccgaac tgcagcccca tcatggtcac caccggaatc aatgcgttgc gcagtgtgtg
     3841 cttgatcacg accagggttt ccgaaacccc tttggcgcgc gcggtgcgca cgtaatcttc
     3901 ctgcatgacc tcgatgaacg acgcccgcgt gaaacgcgcc atcaccgacg ccacggcggc
     3961 acccagggtg atggacggca ggatgtaatg cgccggcgtc gacgcgccca cggtgggcag
     4021 ccatccgagc tgcaccgaga agatctggat cagcatcatg cccagcgcga aggccggaaa
     4081 tgaaatcccc gataccgcca gcgtcatgca gagccg
//
LOCUS       DASTNK010000177        26184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1650714, whole genome shotgun sequence.
ACCESSION   DASTNK010000177 DASTNK010000000
VERSION     DASTNK010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 26184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26184
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650714"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1585
                     /gene="trpE"
                     /locus_tag="VFR20_12795"
     CDS             65..1585
                     /gene="trpE"
                     /locus_tag="VFR20_12795"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU0231439.1"
                     /translation="MTEIEFNALAAEGYNRIPIIAETYADLDTPLAIYLKLAHAGPEG
                     GRNSCLLESVVGGERFGRYSFIGLPARTILRSMGTITEVLLDGKVVEAHEGDPLAFIE
                     SYQQRFKVALRPGMPRFAGGLAGYFGYDTVRHMEPRLGPARKPFPAGQEDGTPDIMLL
                     QIDELVIVDNLAGRTYLIVYADPRQPESYALAKRRLKDLREKLRTPVVIPYAYASLQT
                     QAQRDFKKEDYLAAVHKAKEYIAAGDVMQVQIGQVITKPFRDSPLSLYRALRSLNPSP
                     YMYYWNFDTFQVVGSSPEILVRQETVTEAQRHKSQVTIRPLAGTRKRGATPAEDARLE
                     QELKDDPKERAEHVMLIDLARNDVGRVAETGSVHVTDTMTIERYSHVMHLVSNVTGTL
                     NEGMNNIDVLRATFPAGTLTGAPKVRAMEIIDELEPVRRGIYGGATGYLSYGGEMDVA
                     IAIRTGIVKDGMLYVQAAAGIVADSDPEKEWQETEAKARAVLRAAEQVQFGLDEPI"
     gene            1616..2179
                     /locus_tag="VFR20_12800"
     CDS             1616..2179
                     /locus_tag="VFR20_12800"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU0231440.1"
                     /translation="MLLMLDNYDSFTYNLVQYFGELGEDVKVLRNDQATVEDIAALAP
                     ARICISPGPCSPAEAGISVDLITRFAGKIPILGVCLGHQAIGVAYGGKVVRAMRIMHG
                     KTSNITHTGTDVFTGLPSPLCVTRYHSLAVERGSLPDCLEITAQSEDGEIMGLRHKNL
                     PVYGVQFHPESILSEHGHDMLRNFLAL"
     gene            2195..3226
                     /gene="trpD"
                     /locus_tag="VFR20_12805"
     CDS             2195..3226
                     /gene="trpD"
                     /locus_tag="VFR20_12805"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU0231441.1"
                     /translation="MTITPTEALSRCIEHREIFHDEMLHLMRMLMRGEMSPQIASALL
                     MGLRVKKETVGEITAAAQVMREFALPVTTPDPGNLLDMCGTGGDGSHTFNISTAAMFV
                     AAAAGVRIAKHGNRSASSSSGSADVLEALGANLTLSPEQVAESIERTGIGFMFAPAHH
                     GAMKNVAAVRKELAVRTIFNILGPLTNPAGAANQLMGVFHPDLVGIQVRVLQRLGSRH
                     VLIVHGKDGMDEASLGAATLVGELKDGNVREYEIHPEDFGMAMVSNRAIKVENRDESA
                     KLVLQALDNEAGTARDIVAFNAGLAIYAGNQADSIEQGLKKAFETLASGAARAKLDEF
                     CSYTRTLNA"
     gene            3223..4011
                     /gene="trpC"
                     /locus_tag="VFR20_12810"
     CDS             3223..4011
                     /gene="trpC"
                     /locus_tag="VFR20_12810"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU0231442.1"
                     /translation="MNDILAKIIQTKKIEVATARQMRSESDLLREARSRKDVRGFVRA
                     IEDKIEQGKPAVIAELKKASPSRGVIRENFNPAEIAASYAAHGAACLSVLTDVQYFQG
                     AYDYLRQARAACSLPVLRKDFMIDPYQVVHARALGADCILLIVAALGVTELREFESLA
                     TELGMDVLVEVHDQAELDVALTLETPLVGINNRDLRTFNTSLDTTLSLLSSVPVGKRV
                     VTESGILTRDDVTRMRESSVNAFLVGESFMRAPEPGVALRELFF"
     gene            4024..4488
                     /locus_tag="VFR20_12815"
     CDS             4024..4488
                     /locus_tag="VFR20_12815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU0231443.1"
                     /translation="MNSTISAPCPLCEDDGEVLWRGNALRVIDACAPDHPGYTRVIWN
                     AHVAEMTQLPTAARDRLMRVVWTVEQALRDTLAPDKINLAEFGNQVPHLHWHVIPRWR
                     SDTHFPDAIWAAPITGSDERQTRWATLRSQLLNRLPTYHDALRRALDALTKT"
     gene            4673..6265
                     /locus_tag="VFR20_12820"
     CDS             4673..6265
                     /locus_tag="VFR20_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0231444.1"
                     /translation="MRQQKKQASDNVSATTPLLELRGITKRYPGTLANDNIDLKIMPG
                     ERHALLGENGAGKSTLVSCIYGVIRPDAGQILWRGQPVNVSSPTIAQHLGIGMVFQHF
                     SLFETLTVAQNIALSLRYAGTMRELSEHIAAVSKEYRLSVHPDQHVYSLSVGERQRVE
                     IVRCLLQDPRLLIMDEPTSVLTPGEVDLLFETLRQFAQQGRSILYISHKLEEVRALCT
                     AATVLRTARLVGSCDPTTESAHSLARMMMGSEPPTLKAREAPASSDRVCLRLNGVSHH
                     PREPFGTTLERLNLDVHYGEIVGIAGVAGNGQKELLAALSGESPGLSDDMLQIDGHPA
                     GGLSPAQRRALGLGFAPAERLGRGAVPEMSLVDNTCLTAYQKGLVRHGFIRGAATREM
                     AGAICASFHVVASGVAAAARSLSGGNLQKFIMGREILLTPRVLIAANPTWGVDVGAAT
                     AIRQALMDLVATQQTGILLVSEDLAELFEICDRIAVLYEGNLSRVVAVSETDHDEIGR
                     WMAGLSDTATHTQKEADHAAVS"
     gene            6249..7337
                     /locus_tag="VFR20_12825"
     CDS             6249..7337
                     /locus_tag="VFR20_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0231445.1"
                     /translation="MRLFLEPRIQNSRIMRVASPLLAGVLTIILGGILFAALGREPLS
                     SLYEFFISPISNLYGISTWLLKVAPLLLISMGLTIGFKANVWNIGAEGQYTMGAICGG
                     GLALLFYGHGGWWLLPLMTLAGAAGGALWAAIPAFLKTRLNTNEILTSLLLVYVAQHL
                     LQYLVHGPWRDPQGYNFPQTVMFDAAATFTPIVSGTMLNIATVASILVVPLAWVLMHR
                     MFFGFQVKVSGLSERAADYAGYSRTRMVWCSMLLGGALSGMAGLFEVAGTIGKLQPVI
                     SPGYGFTAIIVAFLGQLEPVGVLLASLLVGLLYVGGDQVQISMHLPSAVAGVFQGLLL
                     FLVLATDVLIRYRVRIVLRAPANLPAEG"
     gene            7339..8265
                     /locus_tag="VFR20_12830"
     CDS             7339..8265
                     /locus_tag="VFR20_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0231446.1"
                     /translation="MVNSIVVSTIVSTVQAGTPLALAALGELIAERAGVLNLGVEGMM
                     LMGAVAGFIAAVATGSPMVGLLVAILAGAFMALLFAFITQHLIANQVATGLALTIFGT
                     GLSAFIGKSYTGISLTAIPSWHIPVLSDLPFVGKILFSYDPLVYVSWLAALAIYGYLY
                     HTRSGLKLRAVGESPHVAHALGYPVVRMRYLAIMFGGALSGLGGAYLSLVYTPLWQEG
                     MVAGRGWIALALVVFATWRPGLVLVGAYLFGFLTVFQYQGQAMGIGIPPDFLAMLPYL
                     ITIVALVFISMDRTRVLLNTPVSLGKPFHPTN"
     gene            8398..9504
                     /locus_tag="VFR20_12835"
     CDS             8398..9504
                     /locus_tag="VFR20_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0231447.1"
                     /translation="MDKTRRDLLKAAAASSAAIGVGGWPGLGMAATKEPLKIGFVYVG
                     PTGDFGWTYQHDLGRKAIIKALGPEAVKTTYVENVGEGSDADRVIRELAAKGNKLIFT
                     TSFGFMDPTMRAARQFPDTIFEMCTGYKLAHNVGTYTPKFIQGRYLTGLIAGHMTKSN
                     IIGYVAPYPIPEVIRGVNAFIIGMHEVNPKARVKVVFINSWFDPGKERLAAETLINQG
                     ADILNKHTDSPAVIQVAESKGVYSFGYDSDMRQFGPHTQLTSIILNWAPFYVETAKQV
                     IAGTWKSRDVWYGFAHDWIQLGPFLPAIPKAVVDQVEKQKQALISGSFKIFKGPLLNQ
                     QGKQVLAAGQTLSDHDIRNMNYFLDGVEGQLPKS"
     gene            10144..11715
                     /locus_tag="VFR20_12840"
     CDS             10144..11715
                     /locus_tag="VFR20_12840"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU0231448.1"
                     /translation="MSFDSLGLAPNLLSAIQKAGFTAPTDVQSAAIPQALQGHDLMVS
                     AQTGSGKTAAFMLPALHRVAQMPAGKGRGVQILVLTPTRELAMQITDATRLYGAHLPD
                     LRTATVVGGVPYGAQLKALSRRVDVLVATPGRLIDHLQSGRVKLSTVHTLVLDEADRM
                     LDMGFIEDIETIVSHTPAERQTLLFSATLDGTIARMAARMMREPVRIEVSGHKQQHTN
                     ITQRLLYADDAGHKQRLLDHLLRDASLNQAIVFTATKRGADDLADRLAEQGFEAAALH
                     GDMNQRQRTRTLGRLQKHQLRVLVATDVAARGIDVQGISHAINYDLPMQPEDYTHRIG
                     RTGRAGHDGLAFTLATHTERHKVRRIEHYIGQPIPLLTIDGLEPKKSVRPTYTDRKGA
                     PRQGDRAGRTGAPGGKPGFSRSARKAGDERSSFVPRTHGYKGATTEHAHRSDNRPESQ
                     AERRATGTPAPRRETSRFDSRPAHAERPALGKPARSDKPAAKWSRAERPSSGARPSTR
                     PGAKPAKHSARRPSF"
     gene            11999..13069
                     /locus_tag="VFR20_12845"
     CDS             11999..13069
                     /locus_tag="VFR20_12845"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEU0231449.1"
                     /translation="MNAALARTEQFCRRFGLRVPILLAPMAGACPPELSIAVANAGGL
                     GACGVLLMPSADIAAWVATFRAHSQGVFQLNMWIPDPPPARDAAHEAKIRTFLKHWGP
                     EAPADAGDARPPELAAQCAAMLDANPPVVSSIMGVYPPDFVRELKARGIAWFAVATTL
                     AEAREAEAAGADAIIAQGMEAGGHRGGFGADAALVRQVGLFSLLPAIVDAVRVPVIAT
                     GGIADARGVAAALMLGASAVQVGTGFLRSNESSIPAAWADALACTAPEDTMVSRVFSG
                     RAGRCVVTDYVRAASAADAPAPAPYPVQRGLTAAMRKQARQDNDLSHMQAWAGQSAAM
                     AQARPAGEILGSLWDGARELLG"
     gene            complement(13191..13967)
                     /locus_tag="VFR20_12850"
     CDS             complement(13191..13967)
                     /locus_tag="VFR20_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0231450.1"
                     /translation="MKSPLVKKCRLPIWGKNVKALEIQQLNTYYGKSHILQGVDLDVA
                     EGELVTLLGRNGAGKSTTLRSIMGLTPARAGHVRIFGTDSTSLPPFRIARLGVGLVPE
                     GRRIFSNLTVEENLKVPVDRPGPWNIASIYKLFPRLAERKGHKGGQLSGGEQEMLSIA
                     RVLLLNPKLLMLDEPSQGLAPLIVQSVFDVIVAARNAGTAVLLIEQNVRAAIEIADRA
                     YVLDRGAITYSGVAAEFGQDEQRVQELAGASAQKWEFPDD"
     gene            complement(13903..14661)
                     /locus_tag="VFR20_12855"
     CDS             complement(13903..14661)
                     /locus_tag="VFR20_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0231451.1"
                     /translation="MSLLEVHNVSQHFGELVAVKDVSLAVEPGELHAIIGPNGAGKTT
                     FFNMISGFYRPTKGHIVFDGADVTAVPPQNRVAMGMARTFQITEVFRELTVRENLRIP
                     VEVTQGLRLKVFRTRQQTRETNERVDELLEAGGLTAKADRYVGELSHGDQRSTEIMMA
                     LALKPKLLLLDEPAAGMGNQETYDTALLIRRLHRKQKLTIILVEHDMRVIFNLADRIT
                     VLTEGEVLEQGTPDEIAASEKVQVAYLGEKREGS"
     gene            complement(14658..15611)
                     /locus_tag="VFR20_12860"
     CDS             complement(14658..15611)
                     /locus_tag="VFR20_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0231452.1"
                     /translation="MNPRIQIRQSIQQPVLWLVLLTMPYWITPLGGYTDLASRLLVIG
                     LAAMATNLLIGHTGVMSFGHAAYFGLGAYATGLTLKYLLPSTPVAFLVGILIGTLGGM
                     IIGYLIVKLRGIYFALATIAFGQVFYFIAFRWNSVTGGDNGLRGFHRLPIDLGFMQLD
                     ITSSLVYYYVVLAVLAICAFAMATILRSPFGHTLQAIRENERRAQFLSIPVNRHLWIA
                     YSISCFFVSVAGALYALLNNFASPHDLYWTQSGDFVLMAVIGGMRSFWGPLLGAAIFV
                     VAQDYLSGVTQDWMFFIGLIFVLVVLLLPRGIAGLFQRRAS"
     gene            complement(15635..16507)
                     /locus_tag="VFR20_12865"
     CDS             complement(15635..16507)
                     /locus_tag="VFR20_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0231453.1"
                     /translation="MTQLVLFNIFNGLVIGAFYALMALGLSLIINLTNVINFAHGGFL
                     ALGAYFAYTLEPYLGFWGALILSPFLAAIVGIVVERVLIRPLYNRDPLYSLLLTFGLA
                     FVLQDSVRFIWGAQGLPLKTPDVLNSSLSPTLFFLTGYRVFMVVLVAVAVAVLFYVLK
                     KTRIGMRIRAGTLDLEMVSALGVDVRILRNLNFGLGIFFAGLAGVLAAGMLGLTPTIG
                     EGLIMPSFVAVIVGGLGSLVGTLVGGLLIGVAAGVVTVFYPSASEASIYVIMAIVLLF
                     RPYGLLGEKGGTKL"
     gene            complement(16685..17944)
                     /locus_tag="VFR20_12870"
     CDS             complement(16685..17944)
                     /locus_tag="VFR20_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0231454.1"
                     /translation="MDDKGSSTAISRRSMLKLAATAGVATLTAPRIVLALGDQPVKFG
                     VDNPLTGTYAITGRNELHGMELAVQEINAKGGILGRPAKLIVEDSTSGDAGVAVQKAH
                     KLIDRDKVDFLIGNVNSGLTEAISQVAHAKGVFMMDPGGHADPITGTECHWDVFQTDP
                     STTLLVNAIAPSLVKRFGKKFYFLVPDYAFGHGLLKAYKENLKKLNAENLGADLIPLG
                     TTDYSSYLIKAQAAKPDVIVILQNGDDQTNVLKQTVQFGLDKRFHIAGANIELETLEA
                     LPPGSRIGNWVIEWYWNQPNVPHVKEFVDAIKKKTGRVPTARTWFGHTAIRACGLAAN
                     NAKSLNPVKMARALDGFTLPPDVALQPYPATYRANQHLLIGTLWAGHAQAHGSAPDDL
                     FHIDVTIESKTIAPTVEETGCKMTWPT"
     gene            18204..18383
                     /locus_tag="VFR20_12875"
     CDS             18204..18383
                     /locus_tag="VFR20_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231455.1"
                     /translation="MAQLARKPLLLAILFVLPLWVALGNFIVAAIVALLLAFLIAMCH
                     ALYGLRRPTRPGSEH"
     gene            18432..19727
                     /locus_tag="VFR20_12880"
     CDS             18432..19727
                     /locus_tag="VFR20_12880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU0231456.1"
                     /translation="MPSHPQDAVTHPPTLDPTSAAHQRAVEAYLVNRIEALPYGYMPF
                     EEWMGLALYAPGLGYYAAGSEKLGPDATRGDFTTAPELTPLFGRTVARQIAQVLEASG
                     SNDVLEFGAGSGALAATLIPALQAMGLAPRYHILEISADLRERQRRRLKDIDADVLWL
                     DKLPDAFTGCVLANEVLDAMPVSLFRWADDGTALELGVSLGCSQSTDHEISLRQRHTQ
                     GIQASLGHPRIDDAPVSADHATPFMFTEKPASPALRQLIEQRVPHMPGYQSEINRQAE
                     AWMRQMGGWLRRGAALIIDYGFPRHEYYHPQRAGGTLMCHFRHHAHSQPLVHAGLQDI
                     TAHVDFTAMADAALDGGLDVLGYTSQARFLLNAGLPEVMEEARAHAADQAARYSDLAA
                     VQQLISEAEMGELFKVLAVGRELDTALMGFTMGDRRHRL"
     gene            complement(19765..20814)
                     /locus_tag="VFR20_12885"
     CDS             complement(19765..20814)
                     /locus_tag="VFR20_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA CCA-pyrophosphorylase"
                     /protein_id="HEU0231457.1"
                     /translation="MVGGAVRDALLGKPAGDRDWVVVGATPENMSARGFVPVGGDFPV
                     FLHPVTKEEYALARTERKSGRGYKGFTFHTGADVTLEADLRRRDLTINAMARSPDGRL
                     VDPWGGQADIHNKILRHVGPAFAEDPVRLLRLARFAARFPEFSVADETLDLARRLVAD
                     GEVDALVPERVWQEVAKGLMTTGPGRMFQVLQSVGALERVAPGLVFTLDVGSQLAIAA
                     RRGLGLAARFGVLCHESAEPGNIGEAWRAPGECRHHATLLPRVLAGVNGRDSGGWLAL
                     LEATDAFRKPQRFLELVDAAGCVLEVDDAAWRLRLEAVRAVDAGAIARAAQGRPDRIR
                     QALGAARLAALESLR"
     gene            complement(20925..23039)
                     /locus_tag="VFR20_12890"
     CDS             complement(20925..23039)
                     /locus_tag="VFR20_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEU0231458.1"
                     /translation="MAFTTQRQQYQKTRAPSRAHPRSGVRLARCMALMAFLGLTGCAS
                     GGMPKAAASPESLGATQPDAPASPAGGASASLSATMTNADARQALIDAHDAMKGRQWS
                     QLAQLVPAASRDPLLGAYAQYWLLRHQLSDPNEPVPTAAVKHFVDSTDNTWLVQDIKA
                     DWIVAAARSADYGTVNRIGPVNVDRTSVECSRLLAQHMTGGRVSATDALAAFKPIRSC
                     WALFDQLVPNKVLGWNDIDPLMRAALEDGKTGNASRLASYLFDASEMRDYRALMKHAR
                     KWLDKQHRPQSRERIELVTLALSRLGRGDRDKNAEYIEQHWARAIPKQDLEWVWGQFG
                     LVAALRVDDDAAKWYRRSGKAPMTDYNHAWQVRTELRQAKISWPRVRDAILKMTARQQ
                     AEPVWTYWYGRALEAEGNRAEADKYFSSIENDYGFYGQLANEALGRQLTIPPEPKPVT
                     TTEEAAARANMGLERAVALFQLGWRHQAVPEWNYALRGMTDRQLLAAAKLADDEHIFD
                     RVINTSLRTQHTVDFKQRFIAPFEGQVAAKAKLINLDVAWVYGLIRQESRFITDARSH
                     VGASGLMQLMPGTAKWVAKKIGMADFSPSTVNDFDTNTILGTNYLNMVLQDLNGSQLL
                     ATAGYNAGPRRPMLWRSKLPGPVEGAIFAETIPFTETRLYVKNVMSNATYYAMMFSGK
                     PFSLKKRLGVVSPEPDRQVALP"
     gene            23076..23705
                     /locus_tag="VFR20_12895"
     CDS             23076..23705
                     /locus_tag="VFR20_12895"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU0231459.1"
                     /translation="MNHTAENTAAALRARLKERRIAQNPIDRHRGALLIRGRLFTWLA
                     TTRARLRQEGRRAPSCIAAFWSLEQEPELQPLLIQWVEEEGLSVALPVIAKPDAPLEF
                     RLWTPDTPMETGAFGIEEPQGDVAPPPDVVLVPTLGFTRAGDRVGYGKGYYDRTLADL
                     KARNHPYTSIGIAWACGDLSGEEYAPAPHDVRLDAVLTDKGWAVPAPVL"
     gene            complement(23898..24731)
                     /gene="metF"
                     /locus_tag="VFR20_12900"
     CDS             complement(23898..24731)
                     /gene="metF"
                     /locus_tag="VFR20_12900"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU0231460.1"
                     /translation="MSIEQAVSLEFFPPRDIAAQEKLVRSAKQLLVVRPSYVSVTFGA
                     GGSTRAGTTNTVNLLQNLGYDAAPHLSCVGSTRAELAEILDGYRAQGIRRLVALRGDM
                     PSGMGPQSGELHYASDLVRFIREHSGDWFNIEVAAYPETHPQATTAAGDLDRFVDKVE
                     AGANSAITQYFFNPDAYFDFVGRARAKGVTVPIVPGIMPITNHSQLVRFSNMCGAEIP
                     RWIRLRLADFGDDRASIRAFGVDVITQLCRSLLDHGAPGLHFYTLNGAEAVLSIWKGL
                     A"
     gene            complement(24731..25066)
                     /locus_tag="VFR20_12905"
     CDS             complement(24731..25066)
                     /locus_tag="VFR20_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEU0231461.1"
                     /translation="MEIILVWILNAVALLIVAYILPGINVASFGSALIAALVLGLLNT
                     LVKPLLILLTLPITIVTLGLFLVVINALVFWFAGSILKGFQVGGFWWAVIGAIVYSLV
                     SGLLSRLMY"
     gene            complement(25190..>26184)
                     /locus_tag="VFR20_12910"
     CDS             complement(25190..>26184)
                     /locus_tag="VFR20_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEU0231462.1"
                     /translation="LLLHLGTKAESNPAVLDKPNGEEERVLFAAIRERLAVDPKWYSS
                     RLAQIRGVTEETTTGVHRLYQMSQKGELAVPAINVNDSVTKSKFDNLYGCRESLVDGI
                     KRATDVMVAGKVAVVCGFGDVGKGCAQALSALRAQVWVTEIDPICALQASMEGYKVVT
                     LDEVADKADIFVTCTGNYHVITRGHMDRMKDQAIVCNIGHFDNEIDVASIENLQWEEV
                     KPQVDHVIFPDGKRIILLAKGRLVNLGCATGHPSFVMSASFTNQTIAQIELFTRGEAY
                     KKGQVYVLPKHLDEKVARLHLNKLGVSLTRLRPDQADYIGVPADGPYKPGHYRY"
BASE COUNT         5010 a         8544 c         7960 g         4670 t
ORIGIN      
        1 ggccgggctt ttttgattcc tgcccggtca acgcggaaat aacaccaata acccgagacc
       61 ctccatgaca gaaatcgaat tcaacgcatt ggccgccgaa ggctacaacc gtatccccat
      121 catcgccgag acctatgccg atctggacac cccgctggcc atttacctga aactggccca
      181 cgccggtccg gaaggcgggc gcaacagttg cctgctggaa tcggtcgtcg gaggggagcg
      241 cttcgggcgt tattcgttca tcgggttgcc tgccaggacg atcctgcgtt ccatgggcac
      301 catcaccgaa gttctactcg atggcaaagt ggtcgaagcc cacgaaggtg accccctggc
      361 gttcatcgag tcgtaccagc agcgattcaa agtggccttg cgcccgggca tgccccgctt
      421 cgccggcggg ctggccggct atttcggcta tgacacggta cggcacatgg aaccgcgcct
      481 ggggccggcc cgcaagcctt ttcccgccgg ccaggaagac ggcacgccag acatcatgct
      541 gttgcagatc gacgaactgg tcatcgtcga caatctggcg ggacgcacct acctgatcgt
      601 ctatgccgac ccgcgccaac cggaaagcta tgcgctcgcc aagcgacgcc tgaaagacct
      661 gcgtgagaag ctgcgcacac cggtggtcat cccctatgcc tatgccagcc tgcagaccca
      721 ggcccagcgc gacttcaaga aggaagacta tctggccgcc gtgcacaagg ccaaggaata
      781 catcgccgcc ggcgatgtga tgcaagtgca gatcggccag gtcatcacca agcccttccg
      841 cgattctccc ctgtcgttgt accgggccct gcgctcgctc aacccgtcgc cctacatgta
      901 ctactggaat ttcgacacat tccaggtggt ggggtcctca cccgagatcc tggtgcgcca
      961 ggagaccgtc acggaagcgc agcgccacaa gtcacaagtc accatccgcc cattggcggg
     1021 cacgcgcaag cgtggcgcca caccggcgga agacgcgcga ctcgaacagg agctcaagga
     1081 cgacccgaaa gagcgcgccg agcacgtcat gctcatcgat ctcgcgcgca acgatgtggg
     1141 ccgcgtggcc gaaaccggca gtgtccatgt caccgacacc atgaccatag agcgttattc
     1201 ccatgtcatg cacctggttt cgaacgtgac gggcacactc aatgaaggca tgaacaacat
     1261 cgacgtgctg cgcgccacgt ttccggccgg gacgcttacc ggcgcgccca aggttcgcgc
     1321 catggagatc atcgatgaac tcgaacccgt gcgccgcggc atctacggcg gcgccaccgg
     1381 ctacctgagc tacggtggcg aaatggatgt ggccatcgcc atccgcaccg gcatcgtcaa
     1441 ggacggcatg ctctacgtgc aggcggcggc cggcatcgtc gccgactccg accccgaaaa
     1501 ggaatggcag gaaaccgagg caaaggcgcg cgcggtgttg cgtgccgcgg aacaggtcca
     1561 gttcggcctg gacgagccca tctgagcccg acacgcaccg cccaaggaga atttcatgct
     1621 gctcatgctc gacaactacg attcgttcac ttacaacctg gtgcagtact tcggcgaact
     1681 cggcgaagac gtcaaagtgc tgcgcaacga ccaagccacc gtcgaggaca tcgccgcgct
     1741 ggcgcccgcg cgcatctgca tttcgccggg gccctgctca ccggccgaag cgggtatttc
     1801 cgtggatctg atcacgcggt tcgccgggaa gatccccatt ctgggcgtct gcctggggca
     1861 tcaggccatt ggcgtcgcct acggcgggaa ggtggtgcgc gccatgcgca tcatgcatgg
     1921 caagacgtcg aacatcaccc ataccggcac cgacgtgttc accggcctgc cctcgcccct
     1981 gtgcgtgacc cgttaccatt ccctggctgt cgagcgtggc tcactgccag actgcctcga
     2041 gatcactgcg cagtccgaag acggcgagat catgggactg cgccacaaaa acctgcccgt
     2101 gtacggcgtg caattccacc cggaatccat cctcagcgag cacggccacg acatgctgcg
     2161 caattttctg gcactttgag gaagagacaa gacaatgacc atcaccccca ccgaagccct
     2221 gtctcgatgc atcgaacatc gcgagatctt ccacgacgaa atgctgcatc tgatgcgcat
     2281 gctgatgcgc ggcgaaatgt ctccacagat cgccagcgcg ttgctgatgg gcctgcgcgt
     2341 caaaaaagaa accgtcggcg agatcaccgc ggcggcgcaa gtgatgcgcg agttcgccct
     2401 gcccgtgacc acacctgatc cgggcaacct gctggacatg tgcggcactg gaggcgatgg
     2461 gtcacacacg ttcaacatct cgaccgccgc gatgttcgtt gcagccgcgg cgggcgtgcg
     2521 cattgccaag cacggcaacc gcagcgcctc gtcgtcctcg ggcagcgccg acgttctgga
     2581 agccctgggc gccaacctga ccctcagccc cgaacaagtc gcggaaagca tcgaacgcac
     2641 cggcatcggt ttcatgttcg cgccggcgca tcatggagcc atgaagaacg tcgcggcggt
     2701 tcgcaaagag ctggccgtgc gcaccatctt caacatcctg ggcccgctca ccaatccggc
     2761 cggcgccgcc aatcagttga tgggagtgtt ccatcccgac ctggtcggca tccaggtgcg
     2821 cgtgctccaa cgcctggggt cgcgccatgt gctgatcgtg catggcaagg acggcatgga
     2881 cgaggcctcg ctgggtgcgg ccacgctggt gggcgagttg aaggacggca acgtgcggga
     2941 atatgaaatc caccccgagg acttcggcat ggccatggtg tcgaaccgcg ccatcaaagt
     3001 ggaaaaccgt gacgaatcgg caaagctggt gctgcaggcg ctcgacaacg aggccggcac
     3061 agcccgtgac atcgtggcct tcaacgccgg cctggccatc tatgccggca accaggccga
     3121 ctcgatcgaa caagggctga agaaagcctt cgaaaccctg gccagcggtg cggcgcgcgc
     3181 gaagctggac gaattctgct cctatacccg gacgttgaac gcatgaacga catcctcgcc
     3241 aaaatcatcc agaccaagaa aatcgaagtc gccacggcac gtcagatgcg cagcgaatcc
     3301 gacctgctgc gcgaagcgcg cagtcgcaag gacgtgcgtg gcttcgtccg cgccatcgaa
     3361 gacaaaatcg aacagggcaa gccggcggtc atcgccgaac tgaagaaggc atcgccttcg
     3421 cgcggcgtga tccgggagaa cttcaatccg gccgagatcg ccgcctcgta cgccgcccac
     3481 ggcgccgcgt gcctgtctgt gctgaccgac gtccagtatt tccagggagc ttacgattac
     3541 ctgcgccagg cgcgcgccgc ttgctcgttg ccagtgttgc gcaaggactt catgatcgat
     3601 ccctatcagg tggtccatgc gcgcgccctg ggggccgact gcattctgct cattgtcgcg
     3661 gcgctgggcg tcacggagct gcgcgaattc gaatccctag ccaccgagct gggtatggac
     3721 gtgctggtgg aagtgcatga ccaggccgag cttgatgtcg cactcactct cgaaacacct
     3781 ctcgtcggca tcaacaatcg cgacctgcgc acattcaaca cctcgctgga caccaccctc
     3841 agccttttgt cgagcgtgcc ggtcggcaaa cgcgtcgtga cggaaagcgg cattctcacc
     3901 cgcgacgacg tgacgcgcat gcgcgaaagc agcgtcaacg cgtttctggt gggtgagagt
     3961 ttcatgcgcg cccccgaacc cggtgtggcg ctgcgcgaac tgtttttctg acgcacggtt
     4021 cgtatgaata gcacgatctc cgcaccatgc ccgctatgcg aagacgatgg cgaagttctg
     4081 tggcgcggca atgcgttgcg cgtcatcgac gcatgcgctc cagatcaccc cggatacacc
     4141 cgggtgatct ggaatgcgca cgtggcggaa atgacgcaat tgcccacagc agcccgcgac
     4201 cgcctgatgc gcgtcgtctg gacggtggaa caggcgctgc gcgacaccct cgcccccgac
     4261 aagatcaacc tcgccgaatt cggcaaccag gttccgcatc tgcactggca tgtcattccg
     4321 cgctggcggt ccgacacgca ctttccggac gccatctggg cggcgccgat aaccggctcc
     4381 gacgaacggc aaacccggtg ggcaactctg cggtcacaat tgctcaaccg cctgccaacc
     4441 taccacgacg cactgcgccg ggcactcgat gccctgacga aaacttgatc gaccaatcaa
     4501 cacttcgtgc cccacgcact ttatactgct ggcaaaccgg atacttgatt ggctcttgca
     4561 ttcaagccca catggccgaa cctgccaggc gcgcgggccg aagcatcagc atcctgggtt
     4621 gaacgttcgt tgaacaactg ccgtggcgcg cgagcagccg gcggaggaag acatgcggca
     4681 acaaaaaaaa caggccagcg ataacgtctc tgcgacaaca cccctgctgg aactgcgggg
     4741 catcaccaaa cgttaccccg gcacactggc caacgacaat atagacctca agatcatgcc
     4801 cggggagcgg cacgcgctgc tcggggagaa cggggcaggc aaaagcacgc tggtcagctg
     4861 tatctacggt gtgatccgac cggatgccgg gcaaatcctg tggcggggcc aacccgtcaa
     4921 cgtgtcgagc cccaccatcg cgcagcatct gggcataggc atggtgttcc agcacttctc
     4981 gttgttcgag acgctcaccg tggcgcagaa catcgccctg agcctgcgct atgccggcac
     5041 gatgcgcgag ctttccgagc acatcgccgc cgtttcaaaa gaataccggc tgtccgtgca
     5101 cccggatcaa cacgtctaca gcttgtcggt cggggagcgt caacgggtcg agatcgtgcg
     5161 ctgcctgctg caggatcctc ggttgttgat catggacgag cccacctcgg tactgacgcc
     5221 gggcgaggtg gacctgcttt tcgaaacgct gaggcaattc gcgcagcaag ggcgaagcat
     5281 tctatacatc agccacaagc tcgaagaggt ccgcgcgctg tgcaccgcgg cgacggtgct
     5341 gcgcacggcg cgcctcgtgg gaagctgcga tccgaccacc gaaagcgcgc acagcctggc
     5401 gcgcatgatg atgggcagtg aaccgccaac cctgaaagcg cgcgaggcgc cggcctccag
     5461 cgatcgggtc tgcctgcggc tcaacggtgt cagccatcat cccagggaac ccttcggcac
     5521 gaccctggag cgcctgaacc tcgatgtgca ttatggcgag atcgtcggca tagcgggcgt
     5581 ggcgggcaac ggtcaaaagg agttattggc ggcgctatcg ggggaaagcc cgggtctgtc
     5641 ggacgacatg ctccaaatcg acggccaccc ggccggcggc ctgagcccgg cccagcgccg
     5701 ggcgctgggc ctgggcttcg ccccggcgga gcgcctgggc cggggtgccg tgcccgagat
     5761 gtcactggtc gacaatacct gcctgaccgc gtaccagaag ggcctggtgc ggcatggatt
     5821 catccggggc gccgccacac gggagatggc cggcgcgatc tgcgcaagct tccacgtggt
     5881 cgcaagcggc gtggccgccg cggcgcgcag cctgtcaggc ggcaatctgc agaagttcat
     5941 catggggcgg gaaatcctgt tgacgccccg cgtgctgatc gccgccaacc cgacctgggg
     6001 ggtggatgtc ggcgcggcca ccgcgatccg ccaggcgctg atggacctgg tggccaccca
     6061 acagaccggt atcttgctgg tgtccgaaga cctcgccgaa cttttcgaaa tctgtgaccg
     6121 catcgccgtc ctgtatgaag gcaatctgtc acgcgtggtg gctgtttccg agacggatca
     6181 cgacgagatc gggcggtgga tggccggact ctccgatacc gcgacgcaca cccaaaagga
     6241 ggcggaccat gcggctgttt cttgaacccc gcatccagaa ctcgcgcatc atgcgcgtcg
     6301 cctcgccatt gctggcgggc gttctgacca tcatcctggg cggtattctg ttcgcggcac
     6361 tgggccgcga accattgtca tcgctctacg agtttttcat cagccccatc agcaacctct
     6421 atggcatcag cacctggctg ctcaaggtcg cccccctgct gctgatcagc atggggctga
     6481 cgatcggctt caaggccaac gtctggaaca tcggcgccga gggccagtac acgatggggg
     6541 ccatctgcgg cggcgggctg gcgttgttgt tttatggcca tggcggctgg tggctgctgc
     6601 cgctcatgac gctggcgggc gccgcgggcg gcgcgctctg ggcggccatt ccggctttcc
     6661 tgaagacacg gctcaacacc aacgagatcc tgaccagcct gctgctggtg tacgtggcac
     6721 agcacctgtt gcagtatctg gtgcatgggc cgtggcgcga cccgcagggc tacaactttc
     6781 cgcagaccgt catgttcgat gccgccgcga ctttcacccc catcgtgtca ggcaccatgc
     6841 tcaacatcgc caccgtggcc agcattctcg tggtgccgct cgcctgggtg ctcatgcatc
     6901 gcatgttctt cggtttccaa gtgaaggtct cggggctctc cgaacgcgcc gccgactacg
     6961 ccggctacag ccgcacacgc atggtctggt gctcgatgct gctgggcggc gcgctctccg
     7021 gcatggctgg cctgttcgaa gtggcgggaa cgatcggcaa gctgcaaccc gtcatctccc
     7081 ccggctacgg cttcacggcc atcatcgtgg cgttcctggg gcagctcgaa ccggtgggcg
     7141 tgctgctcgc cagcctgctg gtgggcctgc tttacgtcgg cggcgaccag gtgcagattt
     7201 ccatgcacct tcccagcgcg gtggcggggg tgttccaggg gctgctgctg tttctggtgt
     7261 tggcgacgga tgtgctgatc cgctatcgcg tgcgcatcgt gctccgggcg cccgcgaacc
     7321 ttcctgcgga gggctgacat ggtcaatagc atcgtggtgt ccaccatcgt atccaccgta
     7381 caggcgggca caccgctcgc gttggcggct ctgggggaac tgatcgcgga acgcgccggc
     7441 gtcctcaatc tgggcgtcga aggcatgatg ctcatgggcg ccgtggccgg cttcatcgca
     7501 gccgtggcga ccggcagccc gatggtgggt ttgctggtgg cgatcctcgc cggggcgttc
     7561 atggccctgc tgttcgcttt catcacccag catctcatcg ccaatcaggt cgccaccggg
     7621 ctcgccctga ccattttcgg aacaggcctg agcgcattca tcggcaaaag ctacactggc
     7681 atctccctga ccgccattcc atcctggcac ataccggtgc tgagcgatct gcctttcgtt
     7741 ggcaaaatcc tgttttccta cgacccgctg gtgtacgtgt cgtggctggc ggccctggcc
     7801 atctacggtt acctgtatca cacccgatcc gggctcaaac tgcgcgcggt cggtgagtcg
     7861 ccgcatgtgg cccacgcgct gggatatccg gtcgtgcgca tgcgttatct ggccatcatg
     7921 ttcggcggcg cgttgagcgg gctgggcggt gcctacttgt cgctggtcta cacgccgtta
     7981 tggcaggaag gcatggtggc gggtcgtggg tggatagccc tggccctcgt tgtgttcgcc
     8041 acctggcgac cgggcctggt gctggtgggg gcttatcttt tcggcttcct gaccgtgttc
     8101 cagtatcagg gccaggccat gggtattggc attccgccgg atttcctggc catgctcccc
     8161 tacctgatca ccatcgtcgc gctggtgttc atctcgatgg atcgcacacg ggtgctgttg
     8221 aatacccctg tatcgctggg caaacccttt catcccacga attaagcacc gcgcgtgtcg
     8281 cagagccccc tcccggccat cgacgcgttt tcctgacggt gaaaaggtag tagattaggt
     8341 ctgttcaaag aattcagtgc gaacgtgcgt cagaaataaa actttcggag actcaatatg
     8401 gataaaacgc gcagagattt actgaaggcg gccgccgcat catcagccgc cattggcgtc
     8461 ggcggttggc ccggcctggg catggccgcc accaaggaac cgctcaagat cggctttgtc
     8521 tatgtcgggc caacgggtga tttcggctgg acctaccagc acgacctggg gcgcaaggcc
     8581 atcatcaagg cactcgggcc ggaagccgtc aagaccacct atgtggaaaa tgtcggtgag
     8641 ggctcggatg ccgatcgcgt catccgcgaa ctggccgcca aaggcaacaa gctcattttc
     8701 accacctcgt tcgggttcat ggaccccacc atgcgggcgg cccggcagtt tcccgacaca
     8761 attttcgaaa tgtgcacggg ctacaagctg gcccacaacg tcggcaccta tacgccgaag
     8821 ttcatccagg ggcgttacct gaccgggctc atcgcggggc acatgaccaa aagcaatatc
     8881 atcggctatg ttgcgcccta tcccatcccc gaggtcatcc gcggcgtcaa tgccttcatc
     8941 atcggcatgc acgaagtcaa tcccaaagcg cgtgtgaagg tcgtgttcat caacagttgg
     9001 ttcgaccccg gcaaggagcg cctggcggcc gagacgctca tcaaccaggg tgccgacatc
     9061 ctgaacaagc acacggattc cccggcggtg atccaggttg ccgagagcaa gggggtctat
     9121 tcttttggat acgattccga catgcggcag ttcgggcccc acacccaact gacatccatc
     9181 atcttgaact gggcgccgtt ctacgttgaa acggccaagc aggtgatagc cggcacgtgg
     9241 aagtcccggg atgtgtggta cggtttcgcc cacgactgga ttcaattggg gccgtttctg
     9301 cccgccattc cgaaggcggt cgtggatcag gtcgaaaagc agaagcaggc ccttatttcg
     9361 ggcagcttca agatcttcaa gggtccgctg ctcaaccagc aaggcaagca ggtgcttgct
     9421 gcgggccaga cccttagcga tcacgacatc cgcaacatga attacttcct ggatggcgtg
     9481 gaggggcagt tgcccaagag ttagcttgtt tttgggcttg ggtatttagg ggattcgacg
     9541 cggttggtat tttaaggcgc tgtctttaaa gcgccatctg gcggggcttg gggcgggtcc
     9601 ggcacggcgt gcttcatccg gatctgtctc gtccaggcgg gcgttgggct aaactcgcgt
     9661 tgagatcagc cgaaggctga tctcaacgct cagacaccgc ccaacgactg cccccgcctt
     9721 cccgaaccag atccggcgca cgccttacgc cagtcccacc ccaagccccg ccagatggct
     9781 cgcggtggcg gcatgctgtg gcgttgagga ttctctgaaa ttgccacacg caacaaaaat
     9841 tcccctttta tttttgttca ggctataatt gcgcaccgca acataatcgc ctcgacattt
     9901 tccacggctt tgcgtaccgt ctgccggccc accccttgcg gggcggaata ctccagcagt
     9961 tgtctgattc tttcagcagc catgtcgagc gccatcgatt cctgaccccg aaccttttcc
    10021 ggcgaggctt ccctgctaca gggcttcgcg caccaggttg tttggtcggc acgatgtcca
    10081 accaacctgt gccccgggct ttgctcctgg agccgcgcgc cgcttgccgg caaaggacga
    10141 ttcatgtctt tcgattctct gggacttgct cccaatctgc tgtccgccat ccaaaaggcg
    10201 ggcttcaccg ctcccaccga tgtccagtcc gccgccattc cgcaggcgtt gcaaggccac
    10261 gacctgatgg tctcggccca gacgggcagc ggcaagaccg cggcattcat gctgcccgcg
    10321 ctgcatcgcg tcgcgcagat gcccgccggc aagggccgtg gcgtgcagat tctggtgctg
    10381 accccaacac gcgaactggc gatgcagatc accgacgcga cccgcctgta tggcgcgcac
    10441 ctccccgacc tgcgcaccgc gacggtggtg ggcggtgtgc cctacggtgc ccagctcaag
    10501 gcgctgtcgc gccgcgtgga tgtgctggtg gccacccccg gacgcctgat cgaccacctg
    10561 cagtcgggcc gtgtcaagct atccaccgtg catacgctgg tgctggacga ggccgatcgc
    10621 atgctggaca tgggcttcat cgaagacatc gagaccatcg tgtcgcacac gccagcggaa
    10681 cgccagactc tgctgttctc ggccaccctg gatggcacga tcgcgcgcat ggccgcgcgg
    10741 atgatgcgag aacccgttcg catcgaagtg tcgggccata aacagcagca caccaacatc
    10801 acccagcgct tgctgtacgc cgacgacgcc ggccataagc aacgcctgct ggaccacctg
    10861 ctgcgcgacg cgagcctgaa tcaagccatt gtgttcaccg ccaccaaacg tggcgccgac
    10921 gatctggccg accgactggc cgagcaaggg ttcgaggccg cggcgctgca tggcgacatg
    10981 aaccagcgtc agcgcacgcg tacgcttggc cgtctgcaga agcaccaatt gcgggtgctc
    11041 gtggcgaccg acgtcgccgc gcgcggcatc gatgtgcaag gcatcagcca cgccatcaac
    11101 tatgacctgc ccatgcaacc cgaggactac acgcaccgca tcggccgtac cgggcgcgcg
    11161 ggccatgacg gcctggcgtt cacgctggcc acacataccg agcgccataa ggttcgccgc
    11221 atcgagcatt acatcggcca acccattcct ctgctgacca tcgacggcct ggagccaaag
    11281 aaatcggtgc gcccgaccta caccgaccgc aagggcgccc cccgacaggg cgatcgcgcg
    11341 ggccgcacgg gcgccccggg cggaaagccc ggcttcagcc gctctgcccg caaagcgggg
    11401 gacgaacggt cgtctttcgt gccgcgcacc catggataca agggggcgac cacggaacac
    11461 gctcatcgct ccgacaatcg ccccgaatcg caggcggaac gacgtgccac cggcacgccg
    11521 gcgccccgac gcgaaaccag ccgtttcgat agtcgtcccg cgcatgccga gcgtccggcg
    11581 ttgggcaagc cggcccggtc ggacaagccg gccgccaaat ggtcacgcgc ggagcgtccg
    11641 tcgtccggcg ctcggccctc aacccggccc ggcgcgaaac ccgccaaaca ttccgcgcgc
    11701 cgcccgtcgt tctgaggcta atggacaata gcccttggcg cgcggcggag cggccccggc
    11761 acataccggg gacagctccg ccgttgtaat tcaacagccg gttcgcctca acgaaccggc
    11821 tgttggttac tgtgcggtgg gtccagcagc gcacacaagg cacaaaccgt ggcgatggct
    11881 ggtactgtcg cgaggatttg caacaccgcc caggccgaaa agacacggtt ttatgtccct
    11941 gatagcgtca aacactgcgc taacatgagg gtattgtcat ctcgccccgg gaacccccat
    12001 gaatgcagct ctcgcgcgca ccgagcaatt ctgtcgtcgt ttcgggctgc gtgtcccgat
    12061 tctgctggcg ccgatggcag gcgcctgccc gcctgaactg tccatcgccg tggccaacgc
    12121 tggtggcctg ggcgcttgcg gtgtgttgct gatgccttcg gccgacatcg ccgcctgggt
    12181 cgcgacattc cgggcgcaca gccagggcgt gtttcaattg aacatgtgga tccccgaccc
    12241 gccacccgcc cgcgatgcgg cgcatgaagc aaaaatccgc accttcctca aacactgggg
    12301 gcctgaagcg ccagcggacg ccggcgacgc cagaccgccc gaactcgccg cccaatgcgc
    12361 ggccatgctc gatgccaacc cgccggtggt gtcctccatc atgggcgtgt atccgcccga
    12421 tttcgtacgc gaactcaagg cccgtggaat agcctggttc gcggtcgcga ccaccttggc
    12481 cgaagcccgc gaagccgaag ccgccggtgc cgatgccatc atcgcgcagg ggatggaggc
    12541 aggcggacac cggggcgggt tcggcgccga cgccgcgttg gttcgacaag tgggcttgtt
    12601 ctcgctgctt cccgccatcg tggacgccgt gcgtgtgccg gtgatcgcca ccggtggcat
    12661 cgcggacgcg cggggcgtgg cggcggcgct catgctgggg gccagcgccg ttcaagtggg
    12721 taccggcttt ttgcgcagca acgaatccag cattcccgcc gcgtgggccg acgccctggc
    12781 atgcaccgcc cccgaagaca caatggtgag ccgtgtattc agtggacgcg cgggccgatg
    12841 cgtcgtcacc gattacgtcc gcgccgcatc ggccgcggac gcccccgcgc cggcaccgta
    12901 tccggtgcaa cgcggactga ccgccgccat gcgcaagcag gccaggcaag acaacgattt
    12961 gagccacatg caggcctggg cgggccagtc ggcggccatg gcgcaggccc ggccggcggg
    13021 cgagatattg gggtcgttat gggacggcgc gcgggaactg ctgggttgac agaaccggct
    13081 cccggtcgca taaaccgccc cgtcggactg aggcacgcac tcaggaatcc ccagagcata
    13141 gacggcctga ggctcgcacc atgccgccgg gccacacaga ccacccgcct tcaatcgtcg
    13201 ggaaactccc acttctgggc gctggcgccg gccagttcct ggacgcgctg ttcatcctgg
    13261 ccgaactccg ccgcgacacc actataggtg atggcgccgc ggtcgagcac gtaggcgcgg
    13321 tcggcgatct cgatggccgc gcgcacgttc tgttcgatca acagaacagc ggtgcccgcg
    13381 ttgcgggcag cgacaatgac gtcgaacaca ctctgcacga tgagcggcgc caacccttgc
    13441 gacggctcgt cgagcatcag cagtttcggg ttaagcagca gcacgcgcgc gatcgacagc
    13501 atctcttgct cgcccccgga caactgccca cccttgtgcc ccttgcgctc agccagccgc
    13561 ggaaaaagct tgtatatgct ggcgatgttc cagggcccgg ggcgatcaac cgggaccttg
    13621 agattttcct cgacggtcag attggaaaag atgcggcgcc cctcgggcac caggcccacc
    13681 cccagcctcg cgatgcgaaa cggcggcagg ctcgtggagt cggtgccgaa tatccgcacg
    13741 tggccggcgc gcgccggcgt cagccccatg atgctgcgca acgtggtgga cttgccggcc
    13801 ccgttgcgac ccagcagggt caccagttcg ccctccgcga cgtcaaggtc tacgccctgc
    13861 aggatgtggc tctttccata gtaagtgttc agttgctgta tctcaagagc cttcacgttt
    13921 ttcccccaga taggcaacct gcactttttc actagcggcg atttcatcgg gcgtgccctg
    13981 ctcaaggact tcgccctcgg tcaggacggt gatgcgatcg gccagattga agatgacacg
    14041 catatcgtgc tcgaccagaa tgatcgtcag tttttgcttg cggtgcagcc tgcggatcag
    14101 caacgcggta tcgtaggtct cctggttgcc cataccggcc gcgggttcgt ccagcagcag
    14161 cagcttgggc ttgagcgcca gcgccatcat gatttccgtt gaacgctggt caccgtgtga
    14221 tagttcgccc acgtagcggt cggccttcgc cgtcaaccca cccgcctcga gcaattcgtc
    14281 gacacgttcg ttcgtctcgc gggtttgctg tcgtgtgcgg aacaccttga gcctcaggcc
    14341 ttgcgtcact tcaaccggaa tgcgcaggtt ttcccgcaca gtcagttcgc ggaagacttc
    14401 ggtgatctgg aaggttctgg ccatgcccat cgccacacga ttctgcggcg gcacggccgt
    14461 gacgtcggcg ccgtcgaaca cgatgtggcc cttcgttggc cgatagaacc cgctgatcat
    14521 gttgaagaac gtggtcttgc ccgcgccgtt cggccctatt atcgcgtgca gctctcccgg
    14581 ctcgacggcc agcgagacat ccttcacggc cacgagctcg ccgaaatgct gggaaacatt
    14641 gtggacttcc agcagactca tgatgccctc ctctggaaca gccccgctat gccacggggg
    14701 aggagcaaca cgacaagcac gaaaatcagc ccgatgaaga acatccagtc ttgcgtgacg
    14761 ccggagaggt agtcttgcgc caccacgaag atggcggccc ccaacagcgg cccccagaaa
    14821 gaacgcatgc cgccaatgac cgccatgagc acgaagtcgc ccgattgcgt ccaatacaga
    14881 tcgtgggggc tggcgaagtt gttgagcagc gcatagagcg caccggcgac agacacgaaa
    14941 aagcaagata tggagtaggc gatccagagg tggcgattga ccgggatgct gaggaactgc
    15001 gcccggcgtt cgttttcgcg gatggcctgc agcgtgtgcc cgaatggcga acggaggatc
    15061 gtcgccatcg cgaacgcaca gatcgcgagg acggcaagca cgacatagta atagaccaga
    15121 ctggacgtga tgtccaactg catgaacccc aggtcgatgg gcaaacggtg aaagccccga
    15181 agcccgttgt cgccaccggt gacggaattc catcgaaatg cgatgaagta gaacacctgc
    15241 ccgaatgcga tggtggccag ggcgaagtag atgccgcgca acttgacgat caggtagccg
    15301 atgatcatcc ccccgagggt gccgatgaga atgcccacca gaaaggccac tggcgtgctc
    15361 ggcaacaggt acttgagcgt cagcccggtc gcgtacgccc ccaggccgaa ataggcggcg
    15421 tggccgaacg acatgacgcc agtgtgcccg atcaggagat tggtggccat ggcggccagg
    15481 ccaatgacca gcagccgcga ggcaaggtcg gtgtatccgc ccagcggcgt gatccagtag
    15541 ggcatggtca gcaacaccag ccatagcact ggctgctgga tggattggcg gatttgaatg
    15601 cgtgggttca tggttcgtgt ttgtatgtgc tggttcacag tttcgttccg cccttttcac
    15661 caagcaggcc gtagggcctg aacaacagca cgatcgccat gatcacgtaa atggatgcct
    15721 cggaggcgga tggatagaac accgtcacga cccccgccgc cacgccgatc agcagcccgc
    15781 cgaccagtgt gccgaccagg ctccccagac cgcccacgat caccgcgacg aaactgggca
    15841 tgatcagccc ctcgccgatg gtgggcgtca ggcccagcat gccggcggcc agcacgccgg
    15901 cgagccccgc gaagaagatg ccgagtccga aattgaggtt gcgcaggatg cggacatcaa
    15961 cccccagtgc cgagaccatt tcaaggtcga gcgtgccggc gcggatgcgc atgccgatac
    16021 gcgttttttt caagacatag aaaaggaccg ccacggccac ggcgacgagc acgaccatga
    16081 acacccggta acccgtcagg aagaacaagg tcgggctgag cgacgaattc aacacgtcgg
    16141 gggtcttgag cggcaacccc tgcgcccccc agatgaagcg cacggaatcc tggagcacga
    16201 acgcaagccc gaacgtcagc agcaggctgt acagcgggtc gcggttgtac aacggccgga
    16261 tcagcacgcg ctcgaccacg atccccacga tggccgccag gaaaggcgac aggatcagcg
    16321 ccccccagaa cccgagatag ggctcgagcg tgtacgcgaa gtacgccccc agggccagaa
    16381 agccgccatg ggcgaagttg atgacattcg tcagattgat gatgagagac aggcccaggg
    16441 ccatgagcgc atagaacgcg ccgatgacca acccgttgaa tatattgaaa aggaccagct
    16501 gggtcacacc aaacctccgt caaggcgaca ctggacaagc atggggcgtg cccacggatg
    16561 cgggacacac ccgtgcttca cgcatggcac taatggtgat ccaaaaatgg agatctgaat
    16621 agagccgcct gcgatgaggc aggcggctcg ggttcccgca aataccgtaa caatcatgag
    16681 atcatcaggt gggccaggtc atcttgcatc cggtttcttc gaccgtcggc gcgatggttt
    16741 tgctctcgat ggtgacatcg atatggaaca ggtcgtcggg cgcgctgccg tgggcctggg
    16801 catggcccgc ccacaacgta ccgatgagca gatgctgatt ggcacggtac gtggcggggt
    16861 agggttgcaa cgcgacatcg gggggcagcg tgaacccatc gagcgcccgg gccatcttca
    16921 cgggattgag cgattttgcg ttattggccg ccagcccgca ggcgcggatc gcggtgtgcc
    16981 caaaccatgt gcgggcagtg gggacccggc cggttttctt cttgatggcg tccacgaatt
    17041 ccttgacatg gggcacgttg ggctggttcc agtaccactc gatcacccag ttgccgatgc
    17101 gggagcctgg cggcagcgct tcgagggtct cgagctcgat attggcgccc gcgatatgga
    17161 aacgcttgtc cagaccgaac tgcacggttt gcttgaggac gttcgtttga tcatccccat
    17221 tctggaggat gacgatgacg tccggtttgg ccgcctgcgc cttgatgaga taggacgaat
    17281 aatcggtggt gcccagcggg atcaggtcgg cgccaaggtt ttcggcgttg agctttttca
    17341 ggttttcttt gtatgccttc aggagcccgt ggccgaaagc gtagtcggga accaggaagt
    17401 agaatttctt gccgaaacgc ttgacgagag agggggcgat cgcattgacc aacaaggtgg
    17461 tcgatgggtc tgtctggaaa acatcccagt ggcactccgt acccgtgatg ggatcggcat
    17521 gcccgcccgg gtccatcatg aatacccctt ttgcatgggc cacttgggag atggcctcgg
    17581 tgaggccgga gttcacgttg ccgatcagga aatcgacctt gtcgcgatcg atgagtttgt
    17641 gcgccttctg caccgccacc ccggcgtcgc cactggtgga atcctcgacg atgagctttg
    17701 ccgggcgccc gagtatgccg cctttggcgt tgatttcctg aacagccaat tccatgccgt
    17761 gcagctcgtt tcgacccgtg atggcataag tgcccgtcaa tggattgtcc acaccgaatt
    17821 tcacgggttg atcgccgagc gccaggacga tgcgcggcgc cgtgagtgtg gccacccccg
    17881 ccgtcgccgc cagcttcaac atcgagcgac ggctgattgc cgtcgaactg cccttgtcat
    17941 ccatttggaa attcctccga tggaggcaag ccagtgtgcg ccgtgtctgc caatgcttgc
    18001 tggacaaatc ctgacggtgg cactaccctt gcctgaaaac cacacccccc tttttctaga
    18061 gtgcgccgaa gccccgcgcc tgtcaacccg tgccagccct gaatacttcc taaacgcagt
    18121 caaattctcc atatctgcat cgaaacgcat aatatcggct gatatcttgc aatatcgata
    18181 attttcgcgt tcatcgctca aaaatggcac aactcgcccg caaaccgctt ctactcgcca
    18241 ttctgttcgt cctgccgctc tgggtcgcgc tgggaaactt catcgttgcg gcgatcgtcg
    18301 ccttgctgct ggcgttcctg atcgccatgt gccacgccct gtatgggctc aggcgaccca
    18361 ccaggccagg ctcggagcat tgaactgata ccataggccg gccgcaacac attcacgttc
    18421 cgtttcaccc catgccctct catcctcaag atgccgtgac ccatccgccc acgctggatc
    18481 ccaccagcgc cgcccatcag cgggccgtgg aggcgtacct ggtcaaccgt attgaagccc
    18541 tgccctacgg atacatgcct tttgaggaat ggatgggcct cgcgctgtac gcgcccggcc
    18601 tgggctatta tgccgccggc agcgaaaaac tcgggccgga cgcgacccgt ggagacttca
    18661 ccacggcgcc cgaactgacg ccgctgttcg ggcgcaccgt ggcacgccag atcgcccagg
    18721 tgctggaggc gagcgggtcg aacgacgtgc tggaattcgg agcaggctcg ggtgcgctgg
    18781 ccgccacgct catccccgcg ctgcaggcca tgggtcttgc gccccgctac cacattctcg
    18841 aaatctcggc cgatctgcgc gaacgacaac ggcggcgcct gaaagacatt gacgccgacg
    18901 ttctgtggct ggacaaactg cctgacgcct tcacaggatg cgtcctcgcc aacgaggtgc
    18961 tggacgccat gccggtgtcc ctgtttcgct gggccgatga cggcacggct ctggaactcg
    19021 gcgtgagcct gggctgcagc cagtccacag accacgaaat cagtctgcgc caacgtcaca
    19081 cccaaggcat ccaggccagc cttggccacc cacgcatcga tgacgccccg gtatccgccg
    19141 accacgccac gccgttcatg ttcaccgaaa agcccgcctc cccggcgttg cgccaactga
    19201 tcgaacaacg cgtgccgcac atgcctggct atcaatcaga aatcaaccga caggcggaag
    19261 cctggatgcg ccagatgggc ggctggctgc ggcgcggcgc ggcgctgatc atcgattacg
    19321 gcttcccccg gcatgagtac taccaccctc agcgtgccgg cggcacgttg atgtgccact
    19381 tccggcacca tgcgcacagc caaccccttg tccacgctgg tctgcaggac atcaccgcgc
    19441 atgtggactt caccgccatg gcggatgccg cgctggacgg cggcctcgac gtgctgggct
    19501 atacctccca agcccggttt ctgctcaatg cggggttgcc cgaggtcatg gaagaagccc
    19561 gcgcccacgc ggccgaccag gcggcgcgct acagcgactt ggccgcggtt caacaattga
    19621 tctcggaggc cgagatgggg gaattgttca aggtgctggc ggttgggcgg gaactggaca
    19681 cggcgttgat gggcttcacc atgggcgatc gccgacatcg gctatgactc gacggcgcgc
    19741 tggtgcgcgg cgccgcgcgg gatgtcagcg cagcgattcc agcgccgcca accgcgccgc
    19801 gcccagcgcc tggcggatgc ggtcggggcg tccctgcgcc gcacgcgcga tggccccggc
    19861 gtccacggcg cgaaccgctt ccagccgcag gcgccaggcc gcgtcgtcga cctcgagcac
    19921 acaaccggcg gcatccacca gttcaagaaa gcgctgcggt ttgcggaacg catcggtggc
    19981 ttccaacagt gccagccaac cacctgaatc gcgcccgttg acgccggcaa gaactcgcgg
    20041 cagcaacgtg gcatggtgcc ggcactcgcc gggcgcgcgc caggcttcgc cgatgttgcc
    20101 aggctcggcg gactcgtgac acagcacgcc aaaccgcgcc gccagcccca gtccgcgccg
    20161 cgccgcgatg gccagctgcg acccgacatc cagcgtgaac accagccccg gtgccacgcg
    20221 ttccagcgcc ccgaccgact gcagcacctg gaacatgcgc cctgggcctg tcgtcatcag
    20281 gcctttggcc acctcttgcc agacgcgctc gggcacgagg gcatccacct cgccatcggc
    20341 caccaaccgg cgcgccaggt ccagggtttc atcggccacg gaaaattccg gaaaccgggc
    20401 cgcgaaacgt gccagccgca ataggcgcac gggatcctcg gcgaatgcgg gcccgacatg
    20461 gcgcaggatt ttgttgtgaa tgtccgcctg gcccccccac ggatcgacca ggcggccatc
    20521 gggactgcgc gccatggcgt tgatggtcag atcccgacgc cgcaagtcag cttccagggt
    20581 gacatcggca cccgtatgaa aggtaaagcc tttatagccc cgccccgact tgcgctcagt
    20641 gcgcgccaag gcgtattcct ctttggtgac agggtgcaga aaaacgggaa agtcaccgcc
    20701 cacgggcacg aagccgcgag ccgacatgtt ttcaggcgtg gcgccaacca cgacccagtc
    20761 gcgatccccc gctggcttgc ccagcagggc gtcgcgcacg gcaccaccga ccatgtagac
    20821 atccagcccg atagtcgcgg tgtcggtacc cgatggtgcg tcaatcgtgg cgcgtgtagg
    20881 gtgcacgccg tctcccggtc gaacgggatc ggaccccgtg gcgctcaagg cagggccacc
    20941 tgccgatcag gctcgggcga caccacgccc aggcgcttct tgagcgagaa gggtttgccc
    21001 gagaacatca tggcgtaata ggtggcattg gacatcacgt tcttgacgta caaacgcgtt
    21061 tcggtgaacg gaatggtttc cgcgaaaatc gcgccttcca ccggccccgg caacttggat
    21121 cgccacaaca tggggcggcg cggcccggcg ttgtaccccg ccgtggccaa caattgcgag
    21181 ccattcaggt cttgaagcac catattgagg taattggtgc ccaggatggt gttggtgtcg
    21241 aaatcattga ccgtcgatgg cgaaaaatcc gccatgccga tcttcttggc cacccacttg
    21301 gccgtgcccg gcatgagttg catcaggccg gatgccccca catgcgagcg cgcatcggtg
    21361 atgaaccggg actcttgacg gatcagccca tagacccacg ccacatccag gttgatcagt
    21421 ttggccttgg ccgcgacctg gccttcgaaa ggcgcgatga accgctgctt gaaatcgacg
    21481 gtgtgctgtg tgcgcagaga cgtgttgatg acacgatcga aaatgtgttc gtcgtcagcc
    21541 agcttggctg ctgcgagcaa ctggcggtcg gtcatgccgc gcaacgcgta gttccattcc
    21601 ggcacagcct ggtggcgcca gccgagttga aacagtgcga cggcgcgttc cagccccatg
    21661 ttggcgcggg ctgcggcctc ttctgtggtg gtgaccggct tgggctcggg cgggatggtc
    21721 agttggcgtc ccagagcctc gttcgcgagc tgcccataaa aaccgtaatc gttctcgatg
    21781 gacgaaaaat acttgtcggc ctcggcccgg ttcccctcgg cctcgagggc tcggccgtac
    21841 caatacgtcc agaccggctc ggcctgctgg cgcgccgtca ttttcagaat ggcatcacgc
    21901 acacgcggcc aactgatttt cgcctggcga agctcggtgc gcacctgcca ggcatggttg
    21961 tagtccgtca tgggcgcctt gcccgagcgc cggtaccact tggccgcgtc atcgtcgaca
    22021 cgcagcgcgg ccaccaggcc gaactggccc cagacccatt ccagatcctg cttcgggatg
    22081 gcccgcgccc agtgttgctc gatgtactcg gcatttttat cgcggtcgcc gcgaccaagc
    22141 cgcgacaggg ccagcgtgac gagctcgatg cgctcacgcg actgcggccg atgctgtttg
    22201 tcgagccatt tgcgcgcatg tttcatgagc gcgcggtagt cacgcatttc cgatgcatcg
    22261 aacagatatg acgcaagacg actggcgtta cccgtcttgc cgtcttccag cgccgcccgc
    22321 atcagcggat cgatatcatt ccaacccaac accttgttcg gcaccagttg atcgaacagg
    22381 gcccagcatg accggatggg cttgaacgcc gccaaggcat cggtggcgga cacccgcccg
    22441 cccgtcatgt gttgcgccag caggcgcgag cattccaccg acgtccggtc gacattcacc
    22501 gggccaatgc ggttgacggt gccgtagtcg gcgctgcgcg cggcggcgac gatccagtcg
    22561 gccttgatgt cctggacgag ccacgtattg tcggtgctgt cgacgaaatg cttgaccgcg
    22621 gcggtcggca cgggctcgtt cgggtcggac aactggtggc gcaacagcca atactgcgca
    22681 tacgcaccca gaagcggatc acggctggcg gcgggaacca gttgggcgag ctggctccat
    22741 tgccggcctt tcatggcgtc gtgtgcgtcg atcagggcct ggcgcgcgtc ggcgttggtc
    22801 atggtggccg acaagctggc tgacgcgccg cccgccgggg aagccggcgc gtcgggctgc
    22861 gtcgccccga gggactcggg tgacgcggcg gccttgggca ttcctccgct tgcgcagcca
    22921 gtcaacccca gaaacgccat gagcgccatg catcgtgcga gacgcacgcc ggatcgggga
    22981 tgggcgcggg aaggtgcgcg ggttttctga tactgttgtc gctgagtggt aaaagccatt
    23041 gaatttctga accccgattt caccaaccac catccatgaa ccacaccgca gagaataccg
    23101 cagccgcgct tcgcgcgcga ctgaaagaac gacgcatagc gcaaaatccc atagatcgcc
    23161 atcggggagc gctattgatt cgagggcgcc tgttcacctg gctggcaacc acccgtgcgc
    23221 gattgcggca agaaggccgc cgcgccccga gttgcatcgc cgcattctgg tccctggaac
    23281 aggagcccga actacaaccc ttgctgattc aatgggtgga agaggaaggt ctgagtgtgg
    23341 cgttgcccgt tatcgccaaa cccgacgccc cgctggaatt ccgcctgtgg acccccgaca
    23401 cacccatgga gaccggtgcc ttcggcatcg aagagcccca aggcgatgtc gcgccaccac
    23461 ccgacgtggt gctggtgccg acgctgggat tcacgcgtgc cggcgatcgc gtgggttacg
    23521 gcaaaggcta ttatgaccgc acactggccg acctgaaagc ccggaatcat ccgtacacaa
    23581 gtatcggcat cgcctgggcc tgcggcgacc tgtctggcga agagtacgcg cccgccccgc
    23641 acgacgtgcg gctcgacgcg gtgttgacag acaaaggctg ggctgttccc gcgccagtcc
    23701 tctgacgagc tcacgcaacg ccgcccaggc cgaaaagacg cggtttcatg tccctgatag
    23761 cgtcaaacac tgcgctagac agaatgtgtt gacctacccc ctcaattacg gctctgcgcc
    23821 tcaggttgta aaaccccaag cgtaatccgc gtctcttgag gtgtggtagg tcaacacatt
    23881 ctgtctagat cccggcttca ggccaaccct ttccagatgc tcagcacggc ttcagcgcca
    23941 ttgagggtgt agaaatgcag ccccggcgcg ccatgatcaa gcaacgaccg gcacagttgc
    24001 gtgatgacat cgaccccgaa agccctgatg gacgcgcggt catcgccgaa atcggccagc
    24061 cgcaaccgga tccagcgcgg gatttcggcg ccgcacatgt tggagaaccg caccagttgc
    24121 gaatggttgg tgataggcat gatgcccggc acgatgggaa ccgtgacgcc ttttgcccgg
    24181 gcccgcccga cgaaatcgaa gtacgcatcg ggattgaaga aatactgcgt gatggcgctg
    24241 ttggcgccgg cttcgacctt gtcgacgaaa cgatccagat cccccgccgc cgtcgtcgct
    24301 tgcgggtggg tttcgggata ggccgcgacc tcgatgttga accagtcgcc actgtgttcc
    24361 cgaatgaaac gcaccaagtc gctggcgtaa tgcagttcgc cagactgcgg ccccatgcct
    24421 gacggcatgt cgccacgcag ggcgacgaga cgcctgatac cctgtgcgcg ataaccatcc
    24481 aggatctcgg ccagctcggc gcgggtcgac cccacgcacg acaggtgtgg cgcggcgtca
    24541 tagcccagat tctgcaacag attgaccgta ttggtcgtgc cggcgcgcgt ggagccgccc
    24601 gcaccgaacg tgacgctgac atagctgggg cgcacgacca gcaactgctt ggccgaccgc
    24661 accagtttct cttgggccgc gatatcgcgg ggcggaaaga attccaggct gaccgcctgc
    24721 tcgatactca tcaatacatc aacctggaca acaagcctga aaccaggcta tagacgatgg
    24781 cgccgatgac ggcccaccag aaaccgccga cctggaaacc cttcaggatc gaccccgcga
    24841 accagaacac cagcgcgttg atcaccacca ggaacaaccc cagcgtcacg atggtgatgg
    24901 gcagcgtcaa caggatgagc aacggcttga ccaacgtgtt gagcagaccc agcaccagcg
    24961 ccgcgatcag cgccgacccg aaggacgcca cattgatgcc cggcaggatg taggccacga
    25021 tcagcagcgc caccgcgttc agtatccaga caagaatgat ttccatggaa cgactccacc
    25081 tgttgcgaag cgccagccac ggtgccattg ggcaaaccgc agccgacggc cccgaggcca
    25141 cgcgtcgcga ccaggcgcgg acgcattaac ggcacggggc tccgacgcgt caataacgat
    25201 aatgacccgg cttgtacgga ccatcggccg gcacaccgat gtaatccgcc tggtcgggac
    25261 gcaggcgcgt gagggacaca cccagcttgt tcaagtgcag gcgcgccact ttttcatcaa
    25321 gatgcttggg cagcacatac acctgcccct tcttgtacgc ctcgccccgc gtgaagagct
    25381 cgatctgggc gatggtctgg ttggtgaacg acgccgacat cacgaacgac gggtgcccgg
    25441 tcgcgcagcc caggttcacc aggcgcccct tggccagcag gatgatgcgc ttgccatcgg
    25501 ggaaaatgac gtggtcaacc tgcggcttca cttcttccca ctgcaggttt tcgatcgagg
    25561 ccacatcgat ttcattgtcg aagtgcccga tgttgcagac gatggcctgg tctttcatgc
    25621 gatccatgtg cccgcgcgta atcacatgat agttgcccgt gcatgtgacg aagatgtcgg
    25681 ccttgtcggc cacctcgtcg agcgtcacca ccttgtagcc ttccatcgaa gcctgcagcg
    25741 cgcagatagg gtcgatttcg gtgacccaca cctgggcccg caacgccgac aacgcctgcg
    25801 cgcagccctt gcccacatcg ccgaacccgc acaccaccgc gaccttgccc gccaccatga
    25861 catccgtcgc ccgcttgatg ccgtcgacca gcgactcgcg gcagccatac agattgtcga
    25921 acttcgactt cgtgaccgaa tcgttcacat tgatggcggg caccgccagt tcgcccttct
    25981 gcgacatctg gtacagacga tgcacccccg tggtggtctc ttccgtgacc ccgcgaatct
    26041 gcgccagacg actggaatac cacttcggat ccaccgccaa acgctcacgg atggccgcga
    26101 acaacacacg ctcttcctcg ccgttcggct tgtcgagcac ggccggattc gattcagcct
    26161 tcgtacccag atgcagcaac agcg
//
LOCUS       DASTNK010000178        12105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1467955, whole genome shotgun sequence.
ACCESSION   DASTNK010000178 DASTNK010000000
VERSION     DASTNK010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12105
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467955"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..616
                     /locus_tag="VFR20_12915"
     CDS             56..616
                     /locus_tag="VFR20_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HEU0231463.1"
                     /translation="MPKLDITAKERSTLRAAAHPLRPVVLIGDRGLSEAVLKEIDLNL
                     KAHQLIKVRVAGDDRDARVAMLETICETLSCAPVHHLGKTLIVYRPDKVAKPADTSPM
                     PTRALRKPSEPHTPKKMAATGAAKTRATRKSERAARKAERDTPTAARARPAASASRAP
                     AHNIPRRTSALSLRAGARKHLSRHEH"
     gene            complement(633..1112)
                     /gene="greA"
                     /locus_tag="VFR20_12920"
     CDS             complement(633..1112)
                     /gene="greA"
                     /locus_tag="VFR20_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU0231464.1"
                     /translation="MSTIPLTARGAVRLQAELHRYKTVERPAVINAIAEARSHGDLSE
                     NAEYDAARERQAFIEGRIQELEGTLSNAQIIDPTALDGVDGRAVFGATVEIEDLESGE
                     RVAYQIVGDVEADIRANLISVSSPVARALIGKSEGDVVEVQVPAGTREYEVLRVSYV"
     gene            complement(1641..2396)
                     /locus_tag="VFR20_12925"
     CDS             complement(1641..2396)
                     /locus_tag="VFR20_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0231465.1"
                     /translation="MRVFITGASSGIGAALARAYAARGAQLGLVGRRIQALDELAEAL
                     PGDHRVYSLDVLDRARLHAAAADFLASGPVDVVIASAGISAGTLTEEADDFEVFKAIM
                     ATNVNATVATFEPFIAAMKRAGRGSLVGISSVAGERGLPGAGAYSASKAAVRLYCESL
                     RVELAASGIRVVTLAPGFIRTPMTAGNPYRMPFLMPVDTFAGKAVAAIDRGDAFRIIP
                     WQMAWVAWLLRRVPRGLYDRFAAGRGRKPRSRG"
     gene            complement(2411..5653)
                     /gene="carB"
                     /locus_tag="VFR20_12930"
     CDS             complement(2411..5653)
                     /gene="carB"
                     /locus_tag="VFR20_12930"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU0231466.1"
                     /translation="MPKRSDIQSILIIGAGPIVIGQACEFDYSGAQACKALKAEGFRT
                     ILVNSNPATIMTDPDTANVTYIEPITWQALEKVIEKEKPDAILPTMGGQTALNCALDL
                     AHHGVLDKHGVELIGANEQAIEKAEDRQKFKQAMTRIGLESAKSGVAHTMEEAWAVQR
                     DIAAQIGTSGFPAVIRPSFTLGGTGGGIAYNPEEFETICRRGLDASPTNELLIEESLL
                     GWKEFEMEVVRDAADNCIIVCAIENLDPMGVHTGDSITVAPAQTLTDKEYQIMRDASI
                     AVLREIGVDTGGSNVQFAVNPANGRMIVIEMNPRVSRSSALASKATGFPIAKVAARLA
                     VGYTLDELRNEITGGATPASFEPSIDYVVTKVPRFAFEKFPQADSRLTTQMKSVGEVM
                     AIGRTFQESFQKALRGLEVGVDGLNQKTTDREKLQVELGEPGPERIWYVGDAFAQGFS
                     LDEVHALTRIDPWFLAQIKEIVDIELALEQRTLAELDFQTLFDLKRRGFSDRRLAFLL
                     DTSEAEVRKLRHQLNVRPVYKRVDTCAAEFSTRTAYMYSTYEDECEAAPTDRKKIIVL
                     GGGPNRIGQGIEFDYCCVHAALALREDGFETIMVNCNPETVSTDYDTSDRLYFEPLTL
                     EDVLEIVNKENPTGVIVQYGGQTPLKLARALEANGVPIIGTSPESIDVAEDRERFQKL
                     LNKLGLRQPPNRTARTEGEAIALAAEIGYPLVVRPSYVLGGRAMEIVHEQTDLERYMR
                     EAVKVSNDSPVLLDHFLNNATEVDVDCLADGQQVVIGGVMEHIEQAGVHSGDSACSLP
                     PYSLSAETITEIKRQTSLMARALNVNGLMNVQFAIQEGDVYVLEVNPRASRTVPFVSK
                     ATGLQLAKIAARAMTGRTLAAQNVQEEVVPPYFCVKEAVFPFVKFPGVDTILGPEMKS
                     TGEVMGVGDSFGEAFVKSQLAAGVLLPESGVAFISVRAQDKPRAVEVAKGLAALGFSI
                     VATRGTAAAIEAAGLSVQVVNKVTEARPHIVDMIKNGEISLVINTVEERRNAIADSRA
                     IRTHALAGRITFFTTIAGAWAAVEGLQYLRQGRGLQVYALQDLHARTRAL"
     gene            complement(5667..6764)
                     /gene="carA"
                     /locus_tag="VFR20_12935"
     CDS             complement(5667..6764)
                     /gene="carA"
                     /locus_tag="VFR20_12935"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU0231467.1"
                     /translation="MADGTVFKGVSIGAKGYSVAEIVFNTAMTGYQEILTDPSYSGQI
                     VTLTYPHIGNTGVNDEDVESSRVHAAGLVVRDCSSRVSNFRATRSLPEYLESQGVVAI
                     AGIDTRKLTRILREKGAQGACILVGEDIDQAVRLAREFSGMSGQDLARVVSVDKSFEW
                     HEGTWRLGEGYDQPQADAYHVVAYDFGVKRNILRLLAQRGCRVTVVPARTPVADVLAL
                     KPDGLFLSNGPGDPEPCDYAIDAAKAAFQKKIPLFGICLGQQIMGLALGGKTVKMKTG
                     HHGANHPVQDVQTGRVFITSQNHGFAVDVESLPANARVTHVSLFDGTLQGFELTDSPA
                     FFFQGHPEASPGPHDIIVLFDKFISLMAAQK"
     gene            complement(7287..8081)
                     /locus_tag="VFR20_12940"
     CDS             complement(7287..8081)
                     /locus_tag="VFR20_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0231468.1"
                     /translation="MSNTRKHRSYMMRLWQVLLLVILIAAWHFASRNINFAFFFGKPA
                     QVVQVIWAWFVTDGNIYHHLGVTLTETVLAFIIGTATGLACGLWLGLSRGASALLEPY
                     VKALNSMPRVILAPIFGMWFGLGIWSKVALAVTLVFFIVFFNVYQGVREVSTTLLDNA
                     RMLGASRNQLLRHVYIPSATSWVFSSLHTSVGLAFVGAVVGEYLGSASGVGYLILQAE
                     GTLDVNTVFAGIVVLTIFALILDGIVGLVEERFLVWKPKAGETEKI"
     gene            complement(8085..8894)
                     /locus_tag="VFR20_12945"
     CDS             complement(8085..8894)
                     /locus_tag="VFR20_12945"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0231469.1"
                     /translation="MPDAALLLDHITCTFKSNRGDNASYTAVKDAHIAVAPGEFVSVV
                     GPTGCGKSTLLNVGAGLLAPSSGQIKVFGEPLKGVNQRAGYMFQGEALLPWRNALDNV
                     TAGLEFAKVPKADAVNRGREWLKRVGLGGFENRYPHQMSGGMRKRVMLAQTLIRNPDI
                     ILMDEPFSALDIQTRQLMENEVLDLWMAQRKAVLFITHDLDEAIAMSDRVVVLSAGPA
                     THPIGEFIIDIPRPRDVTEVRGNPRFIELHAAIWDVLREEVLKGYAQQKRA"
     gene            complement(8887..9927)
                     /locus_tag="VFR20_12950"
     CDS             complement(8887..9927)
                     /locus_tag="VFR20_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0231470.1"
                     /translation="MSLSRRDLLKLAGAAGAATVVPGISRAASAEKKDVIIAVGGQAL
                     IYYLPLSIARDLHYFKDEGLNAKVMDFAGGSKALQAVVGGSADVVSGAFEHTIFLQSK
                     GQMYRTFAQQGRAPMIVLGVSTKTMPHYKTPADLKGKKIGVTAPGSSTNMMASFFLAK
                     HGLKPTDVSYIGVGAGQGAITALRTGQIDAIANLDPVISTLVKANELKIIADTRTLKD
                     TREIFGGDMPSGCLYASQKFIDENPKTAQALANAIVRADRWIQENSFDKIAATVPKQY
                     LLGDPDLYKLALKGNREALSPDGLVPAGGPETALKALAAYVPHFQADKIDISKVWTNA
                     YALKADKKYPHA"
     gene            complement(10189..10797)
                     /gene="recR"
                     /locus_tag="VFR20_12955"
     CDS             complement(10189..10797)
                     /gene="recR"
                     /locus_tag="VFR20_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEU0231471.1"
                     /translation="MEPALPEPEPLTALIQALRRLPGVGVRSARRMAYHLMQHDLAGA
                     DQLSRALSDAVHTLGHCPRCNSFTEGDVCATCANPKRDPSLLCVVETPSDQNMIESSH
                     GYRGLYYVLMGRLAPLEGVGPEDLHFDRVIQRATDGVVREVILATNFTAEGETTAHYL
                     AESLRARGLTVSRLARGVPAGSELEYVDAGTIAWALMERRST"
     gene            complement(10797..11123)
                     /locus_tag="VFR20_12960"
     CDS             complement(10797..11123)
                     /locus_tag="VFR20_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU0231472.1"
                     /translation="MMKGQIAGLMRQAQQMQENMKKAQDALAEIVVDGASGGGLVKVS
                     MTCRHDVRRVAIDPSLLGDDKDMLEDLVAAAFNDALRKAETTSQEKMAAVTAGMPLPP
                     GMKLPF"
     gene            complement(11230..11904)
                     /locus_tag="VFR20_12965"
     CDS             complement(11230..11904)
                     /locus_tag="VFR20_12965"
                     /inference="COORDINATES: protein motif:HMM:NF023592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HEU0231473.1"
                     /translation="MDDRGPAPVAQSTISRATTDGSPPSGNDQPSDTAPDDGPPESWY
                     EDAGFAPDDTGFTPSDDPDEFETLAPDVERAMGAMPGRSQAEQASPSLRAMTPDAWPA
                     LAASLPLTGLAAELARQSEWLEADDHRIALRVAVRSLVETHGRARLRTVLSEHFGTAV
                     QLDIECGATGDATAHAVAQARSEALQRQAEQDVDADPFVRSLIDEFGGTIIPGSVRAV
                     PDDKAA"
BASE COUNT         2295 a         3912 c         3740 g         2158 t
ORIGIN      
        1 tgctgtatcc actcttttga gaattttttc ttggccattc ccgtacaatc tcaatatgcc
       61 aaaactcgat atcaccgcca aagagcgcag caccctacgt gccgccgcgc accccttgcg
      121 gccagtcgtc ctgatcggtg accgcggcct gtccgaggcc gtgctcaagg aaatcgacct
      181 gaacctcaag gcccaccaac tcatcaaggt ccgcgtggcg ggtgacgacc gcgacgcacg
      241 cgtcgccatg ctcgaaacca tctgcgaaac gctatcatgc gcgcctgtgc accatctggg
      301 caaaacgctg atcgtctacc ggcccgacaa ggtcgccaag cccgccgaca cctcgcccat
      361 gcccacccgg gccctgcgca aaccgtccga gccgcacacc ccaaagaaaa tggcagcaac
      421 gggcgccgcc aaaacccgcg ccacccgcaa gagcgaacgc gcggcccgga aagccgagcg
      481 cgacaccccc acggcggccc gcgcccgtcc ggcggcatcc gcctcgcgcg caccggcgca
      541 caatattccc cgccgaacca gtgccctcag cctcagagcc ggggccagga aacacctgtc
      601 ccgccacgaa cactgagcca catccggcga cctcaaacgt agctgacccg caggacctcg
      661 tattcgcgag tgccggcggg cacctggacc tcgacaacgt ctccctcgct cttgccgatg
      721 agcgcccgag ccaccggact ggagacggaa atcaggtttg cgcggatgtc ggcctcgaca
      781 tcgccgacaa tctgatacgc aacacgctcg cccgattcca ggtcttcgat ttcaaccgta
      841 gcgccgaaca ccgcccggcc atcgacgccg tcaagcgcgg tgggatcgat gatctgggca
      901 ttggacagcg tgccttccag ttcctggata cgcccttcga tgaaggcctg gcgctcgcgc
      961 gcggcgtcat attcggcgtt ttcggacaaa tcgccatgag agcgggcttc ggcgatcgca
     1021 ttgatcaccg ccggacgttc caccgtcttg tagcgatgca gttctgcctg caggcgcaca
     1081 gcgccgcgag ccgtcaatgg aatcgtagac atacctattc ctgaatcaaa aagtaaaaag
     1141 acgctccgcc acagggagcg ccccgttgat acgagcgtga cggaaaaacc gccgatttga
     1201 ggaaagcccc ggacagcggc gcgaagatga ccagccgctg gcctgtcgtt tacaccaccc
     1261 cggatggggc gatgcgcaac acccgccacc tgaggcgtcg cgccccaggc atagcctgcc
     1321 cggatcaaaa caaatccccg gctcggtgga accggggaca tagggctatt gtcggcaaag
     1381 atcgaacaaa ttgcaagcct cgggagagga tataagggtt ttcccttata ttttctataa
     1441 gtttttgaaa aaaaacaaaa aataaaataa aaatccccac catgggtagt gaaaacagac
     1501 tactcgcatg ggctgaaatg atccccttgc agggggctcc gccgagaggc cggacatcct
     1561 cccccctgcc ctcccgccac cccacggggt tgatttccgg acaaggcggg gacaagacgc
     1621 ggcggccatt tcccagccgc tcagcctcgc gatcggggct tgcgccctcg gccggcggcg
     1681 aagcgatcat agaggccgcg cggaacacgg cgcaacagcc aggcgaccca cgccatctgc
     1741 cagggaatga tccggaacgc gtcaccacga tcgatggcgg cgaccgcctt gccggcgaag
     1801 gtatcgacgg gcatgagaaa cggcatgcgg tagggattgc ctgccgtcat gggcgtccgg
     1861 atgaaccccg gcgccaaggt caccacccgg ataccggagg ccgcaagctc cacgcgcagg
     1921 ctctcgcaat acagacgcac cgcggctttt gaagcgctgt atgcgccggc gcccggcagg
     1981 ccccgctccc cggcgacact ggaaatgcca accagcgagc cccggccggc gcgcttcatg
     2041 gcagcgatga acggctcgaa tgtagccacc gtggcgttca cgttcgtcgc cataatggct
     2101 ttgaagacct cgaaatcatc ggcttcttcc gtgagcgtcc ccgcgctgat gccggcgctg
     2161 gcgatcacca cgtcgaccgg tcctgacgcc aggaaatcag cggccgccgc gtgcaagcgc
     2221 gcacggtcga gcacatccag ggaatagacg cggtgatcgc cgggaagcgc ctcggccaac
     2281 tcgtcgagcg cctggatacg gcgccccacc agccccagtt gcgcgccacg cgcggcatag
     2341 gcacgggcca gcgccgcacc aatgccgctg ctggccccgg tgatgaagac gcgcatggcg
     2401 cgcgcgcccg tcagagggct ctggtgcgcg cgtgcagatc ctgcagcgcg tagacctgca
     2461 ggcccctgcc ctggcgcaga tactgcaacc cttccacggc cgcccaggcg ccggcgatgg
     2521 tggtgaagaa ggtgatgcgc ccggccaggg catgtgtgcg gatggcgcgc gaatcggcaa
     2581 tggcgttgcg ccgctcctca acagtgttga tcaccaggga gatctccccg ttcttgatca
     2641 tgtcgacgat atgggggcgc gcttccgtga ccttgttgac cacttggacc gacaatcccg
     2701 cggcttcgat ggccgcggcc gtgccgcgcg tcgccacgat cgaaaagccc agcgccgcca
     2761 ggcccttggc cacttccacc gcccggggct tgtcctgggc acgcacgctg atgaaagcca
     2821 cacccgattc gggcagcaac actccggcgg ccagttgaga cttcacgaac gcttcgccga
     2881 agctgtcgcc cacgcccatg acctcgccgg tggacttcat ttccggaccc aggatggtgt
     2941 cgaccccggg gaacttcacg aaggggaaca cggcttcttt gacacagaaa tacggcggca
     3001 ccacttcttc ctgcacattc tgcgcggcca gcgtgcggcc cgtcatggcg cgcgccgcga
     3061 tcttggccag ttgcaggccc gtggccttgg acacgaacgg caccgtgcgc gacgcgcgcg
     3121 ggttgacctc gagcacatag acgtcgcctt cctgaatggc gaactgcacg ttcatcaggc
     3181 cgttgacgtt gagcgcccgc gccatcaaag aagtctgacg tttgatttca gtgatggttt
     3241 ccgccgacag ggaatacggc ggcaggctgc aggccgagtc cccggaatgc actccggctt
     3301 gctcgatgtg ctccatgacg ccgccgatga ccacctgctg accatcggcc aggcagtcca
     3361 cgtccacctc ggtggcgttg ttgaggaaat ggtcgagcag cacgggggaa tcgttgctga
     3421 ccttgaccgc ctcgcgcatg tagcgctcca ggtcggtctg ctcgtgcacg atttccatag
     3481 cgcgcccacc caatacataa ctggggcgca ccaccagcgg atagccgatc tcggcagcca
     3541 gggcgatggc ctcgccctca gtgcgcgcgg tgcggttggg cggctggcgc aggcccagct
     3601 tgttcagcag cttctgaaac cgttcgcggt cttcggcgac gtcgatggat tcggggctgg
     3661 tgccaatgat gggcacccca ttggcttcga gcgcgcgcgc cagcttcagc ggcgtctggc
     3721 caccatactg cacgatgacg ccggttggat tttccttgtt gacgatctcg agcacgtctt
     3781 ccagcgtgag cggttcgaaa tacaggcgat ccgacgtgtc gtagtcggtt gagacggtct
     3841 ccgggttgca attgaccatg atggtctcga acccgtcctc acgcagcgcc agcgcggcgt
     3901 gcacacagca gtagtcgaac tcgatgccct ggccgatgcg gttcggaccc ccgcccagga
     3961 cgatgatctt cttgcgatcg gtgggcgcgg cctcgcactc gtcttcatag gtggaataca
     4021 tgtaggccgt gcgcgtggag aattccgccg cgcaggtgtc gacgcgcttg tacacgggac
     4081 gcacgttcaa ctggtggcgc agcttgcgca cctcggcctc ggacgtgtcc agcaggaacg
     4141 ccagacggcg gtcggaaaac ccgcggcgct tcagatcgaa cagcgtctgg aaatccagct
     4201 cggccagcgt gcgctgctcg agtgccagtt cgatatcgac gatttccttg atctgcgcga
     4261 ggaaccacgg gtcgatgcgc gtgagagcat gcacctcgtc caggctgaac ccctgggcga
     4321 aggcgtcgcc cacgtaccag atacgctcgg ggccgggttc gcccagttca acctgcagtt
     4381 tttcgcgatc ggtggttttc tgattgagac cgtccacccc cacttccagc ccgcgcaggg
     4441 ctttctggaa agactcctgg aaggtgcgtc cgatggccat gacctcgccc accgacttca
     4501 tctgcgtggt gaggcgcgag tcggcctgcg gaaacttttc aaatgcgaaa cgcggcacct
     4561 tggtgacgac gtaatcgatg gagggttcga acgaggcagg cgtggcgcca cccgtgatct
     4621 cgttgcgcaa ttcgtcgagc gtgtagccga ccgccagccg ggccgccacc ttcgcgatag
     4681 ggaaaccggt agccttggag gccagcgccg aagaccgcga cacacgcggg ttcatttcaa
     4741 tgacgatcat gcggccgtta gcgggattga cggcgaactg cacgttcgaa ccacccgtat
     4801 ccaccccgat ttcgcgcagc accgcgatcg aggcgtcgcg catgatctgg tattccttgt
     4861 cggtcagcgt ctgcgcgggc gccaccgtga tggaatcacc ggtgtgcacc cccatggggt
     4921 cgagattctc gatcgcgcac acgatgatgc agttgtccgc cgcgtcgcgc accacctcca
     4981 tctcgaactc cttccagccc agcaacgatt cctcgataag aagttcgttg gtgggcgagg
     5041 cgtccagccc ccgccggcag atggtctcga attcctcggg gttgtaggca ataccaccgc
     5101 ccgtgccgcc cagcgtgaag ctgggccgga tcacggcggg aaaacccgac gtgccgatct
     5161 gggcggcgat gtcgcgttga acggcccacg cctcttccat ggtgtgcgcc acacccgatt
     5221 tggccgactc cagaccgatg cgcgtcatgg cctgcttgaa cttctggcgg tcttccgcct
     5281 tttcgatggc ctgctcgttg gccccgatga gctcgacacc gtgtttgtcg agtaccccgt
     5341 gatgggccag atcgagggcg cagttcagcg ccgtctggcc acccatcgtg ggcaggatcg
     5401 cgtcgggctt ttccttttcg atgaccttct cgagcgcctg ccacgtgatg ggctcgatgt
     5461 aggtgacgtt ggccgtatcc gggtcggtca tgatggtcgc cggattgctg ttgaccagaa
     5521 tagtgcggaa cccctcggcc ttgagcgcct tgcaggcctg tgccccggag tagtcgaatt
     5581 cgcacgcctg gccgatcacg atcggcccgg cgccaatgat aagaatgctt tgaatgtctg
     5641 aacgctttgg catggtgctg tctttcttat ttctgggcgg ccatcaggct gatgaatttg
     5701 tcgaagagca cgatgatgtc gtgcggaccc ggactggcct cggggtggcc ctggaagaaa
     5761 aaggcggggc tgtccgtgag ttcgaacccc tgcaaggtgc cgtcgaacaa cgacacgtgg
     5821 gtcacccggg cattggccgg cagcgattcg acatcgaccg cgaacccgtg gttctgactg
     5881 gtgatgaaca cacgcccggt ctgaacgtcc tgaaccgggt gattcgcgcc atggtggccg
     5941 gtcttcattt tgaccgtttt gccgcccagg gccaggccca tgatctgctg gccaaggcag
     6001 atgccgaaaa gcgggatctt cttctggaac gcggccttgg ccgcgtcaat ggcgtaatcg
     6061 cacggttccg gatcacccgg gccattcgac agaaacagcc cgtctggctt gagcgccaga
     6121 acgtcggcaa ccggtgtgcg cgccggcacg acggtcacgc ggcaaccacg ctgggcgagc
     6181 aaccgcagaa tgttgcgctt gacgccgaag tcgtaggcca ccacatgata ggcatcggct
     6241 tgtggctgat cgtagccctc gccgagccgc caggtgccct catgccattc gaatgacttg
     6301 tccacggaca cgacccgggc cagatcctgg cccgacatgc ccgagaactc ccgcgcgagg
     6361 cgcacggctt ggtcgatgtc ctcacccacc aggatgcagg caccctgggc acccttttca
     6421 cgcagaatgc gggtcaactt gcgggtgtca atgcccgcga tcgccaccac accctgcgac
     6481 tcgaggtatt caggcagcga tcgcgtggcg cggaagttcg agacccgcga agagcagtcg
     6541 cgcaccacca ggccggcggc gtggacacga gaggattcga cgtcttcatc gttgacgccg
     6601 gtgttgccga tgtggggata cgtcagcgtg acaatctggc cgctatagct gggatcggta
     6661 aggatctctt gatagccggt catggcggtg ttgaacacga tctcggcgac gctgtacccc
     6721 ttggcgccga tcgacacccc tttgaaaacc gtaccgtccg ccagggcaag gatcgcaaaa
     6781 gaaggcttct gagcctcttc ggaaaataat gacggcagca cggtaaaccc caaatattgt
     6841 agtgacgaaa cggaaataaa actttcagat tataccgtgg agcccgcggc cggccgccgc
     6901 gcgcccgcgc gaacccgcca acatcaaggc tggcgcggct tcaggattct ttgcacagtt
     6961 ttggccgaga ggggctccgg ccggggatca tccatgacac gacccaggga gcagatgcga
     7021 gtcagtgagc tcccaacatg ccaccaccgc aagctgtcca ccggggcttg gggtgggacc
     7081 ggcgtaaggc gtgcgccgaa tctggctcgc ccaggcgggg gcagtcgtcg ggcggtgtct
     7141 aagcgttgag gtcagcccga cgcccgcctg gacgagccag attcggatca agcacgccgt
     7201 gccggccccg ccccaagccc cggtggacag cgccttcaga taccaacagc gccaaacccg
     7261 gcacacacct gaaccagcac gaagcgctag attttctcag tttccccggc cttcggcttc
     7321 cacaccagga accgctcttc aaccaaaccg acgatcccgt caagaatgag cgcgaaaatc
     7381 gtcaacacga cgatacccgc gaacaccgtg ttgacgtcca acgtgccctc ggcctgcaga
     7441 atgagatagc caaccccgct ggccgacccc agatattccc ccaccacggc gccgacgaac
     7501 gccagcccca ccgaagtgtg cagactggag aacacccaac tcgtggccga cggaatgtac
     7561 acatggcgca gcagctgatt gcgactggcc ccaagcatgc gcgcattgtc gagcagcgtg
     7621 gtgctgactt cgcgcacacc ctggtacaca ttgaagaaca cgatgaaaaa gaccagggtg
     7681 accgccagcg ccaccttgga ccagataccc aggccaaacc acatgccgaa gataggggcc
     7741 aggatgaccc gaggcatgga attgagcgcc ttgacatagg gttcaagcag tgcgctcgcg
     7801 ccacgggaca gccccagcca caaaccacac gccaggccgg tggcggtacc gataatgaag
     7861 gccagcacgg tttccgtgag tgtgaccccc agatgatggt agatattgcc gtcggtcacg
     7921 aaccacgccc agatgacctg aaccacctgc gcgggtttgc cgaagaaaaa cgcgaaattg
     7981 atattgcgcg acgcgaaatg ccacgcggcg atgaggatga cgagcagcag cacctgccac
     8041 aggcgcatca tgtaagaacg gtgtttgcgt gtgttggaca tggatcaggc ccgtttctgc
     8101 tgggcatagc ccttcaggac ttcctcgcgc aggacgtccc agatggcggc atgcagttcg
     8161 atgaagcgcg gattgccgcg cacctcggtc acgtcgcgcg gccggggaat gtcgatgatg
     8221 aactcgccga tgggatgcgt ggccggcccc gccgacagca ccaccacgcg gtcgctcatg
     8281 gcaatggcct catccaggtc atgcgtgatg aacaagaccg ccttgcgctg cgccatccag
     8341 agatccagca cctcgttttc catgagctga cgggtctgga tgtcaagcgc ggagaacggc
     8401 tcgtccatga ggatgatgtc ggggttgcgg atcagagtct gggcgagcat gacacgcttg
     8461 cgcatgcccc ctgacatctg atgcgggtag cggttctcga aacccccaag ccccacccgc
     8521 ttgagccact cccgcccccg gttcacggca tcggccttgg gaaccttggc gaattccagg
     8581 ccagccgtga cgttgtccag cgcgttgcgc cagggcagca acgcctcgcc ctggaacatg
     8641 tagcccgcgc gctggttcac gcccttgagc ggctcgccaa acaccttgat ctgccccgaa
     8701 gacggggcca gcagccccgc ccccacgttc aacaaggtgg atttgccgca gcccgtgggc
     8761 ccgaccaccg agacgaactc gccgggcgcg acggcgatat gggcgtcctt gacagcggtg
     8821 taggacgcgt tgtcgccccg gttggacttg aaggtgcagg tgatgtggtc gagcaggagc
     8881 gcggcgtcag gcatgcggat atttcttgtc ggctttcaag gcgtaggcgt tggtccagac
     8941 cttggagatg tcgatcttgt cggcctggaa gtgcggcacg tacgccgcca gagccttgag
     9001 ggctgtttcc gggccgccgg ccggcaccag gccatcgggc gacagggctt cgcggttacc
     9061 cttgagagcc agtttgtaga gatccggatc gcccagcaga tattgcttgg gtacggtggc
     9121 ggcgatcttg tcaaagctgt tttcctggat ccagcgatca gcgcgcacaa tggcgttggc
     9181 cagggcctgg gccgtcttcg ggttctcgtc gatgaatttc tgcgatgcgt acaggcatcc
     9241 cgagggcatg tcgccaccga atatttcccg cgtgtccttg agggtacgcg tgtcggcgat
     9301 gatcttcagc tcgttggcct tgaccagggt gctgatgacc ggatccaggt tggcgatggc
     9361 gtcgatctgg ccggtgcgca atgccgtgat ggctccctgc cccgccccca cgccgatata
     9421 agagacgtcg gtcggcttca ggccgtgttt ggccaggaag aaactggcca tcatgttggt
     9481 ggacgagcca ggcgccgtca cgccgatttt cttgcccttg aggtcggcgg gcgtcttgta
     9541 gtgcggcatg gtctttgtcg acacaccgag gacgatcatg ggcgcgcggc cttgttgggc
     9601 gaacgtgcga tacatctgtc ccttggactg caggaagatg gtgtgctcga acgcgcccga
     9661 caccacgtcg gcgctgccgc ccaccacggc ctgaagggcc ttggagccac ccgcgaagtc
     9721 catgaccttg gcgttgagcc cctcgtcctt gaagtagtgc aggtcgcgcg cgatgctgag
     9781 cggcaggtaa taaatgagcg cctggccgcc cacggcgatg atgacgtctt ttttctcggc
     9841 gctggcggcc cggctgatac ccggcaccac tgttgccgcg cccgcggcac cagccagttt
     9901 gagcaaatct cggcgtgata aagacatgag cgacctcctt tagtcgtacg gaacttcaat
     9961 gatcgtcggc gagttgcgca tgatgcgttt cgaggaaatt cattcctgaa aaacgggccc
    10021 gctcgacatt attcgccgtt gtttttcaaa cccggcaaaa aaacccgttc aattatgcca
    10081 tcaacccatc cgggtacaag cgtatctttc atactggtat tggcaatgcc acctacggca
    10141 tagtttgaat ttggccggtt gcatcatctg ggcccggccc gacggacttc aggtggaacg
    10201 acgctccatc agcgcccagg cgatggttcc cgcgtccacg tattcgagct cgctgccggc
    10261 cggcaccccg cgcgccaggc ggctgaccgt caggccgcgc gcgcgcagcg attccgccag
    10321 atagtgcgcc gtggtctcgc cctcggcggt gaaattggtg gccagaatca cctcccgcac
    10381 caccccatcg gtggcgcgct ggatcacgcg atcgaagtgg agatcttccg gcccgacacc
    10441 ctcgagaggc gccagacggc ccatcagcac atagtacaaa ccccgataac catggctgga
    10501 ttcgatcatg ttctgatcag acggcgtctc gaccacgcaa agcagcgagg ggtcgcgctt
    10561 ggggttcgcg cacgtggcgc agacatcacc ctcggtgaag ctgttgcagc gcgggcaatg
    10621 gcccagtgtg tggacagcgt ccgacaacgc ccgtgacaat tgatcggccc ccgccaaatc
    10681 gtgctgcatc aggtgatagg ccatgcggcg ggccgatcga acccccacgc ccggcagtcg
    10741 ccgcaacgcc tggatgagcg ccgtcagggg ctcgggctcg ggcagggcag gttccatcag
    10801 aacggcaact tcatcccggg tggcagtggc atgccggccg tgaccgcggc cattttttcc
    10861 tgggaggtgg tctcggcctt gcgcagcgca tcgttgaacg ccgcggccac gaggtcttcg
    10921 agcatgtcct tgtcgtcgcc cagcagactg ggatcgatgg cgacccggcg cacgtcatga
    10981 cggcaggtca tgctgacctt caccaggccg ccgcccgatg cgccgtcgac gacgatctcc
    11041 gccagggcgt cctgggcctt cttcatgttt tcctgcattt gctgggcttg tcgcatcaag
    11101 cccgcgattt gacctttcat catggtgact tcatcctgac tgaaataaga actggaagtg
    11161 aataatggtt gaatctgttc atgccgccag gcgccgggaa cgagccggcc gaccttctac
    11221 gacacggcac taggccgcct tgtcatccgg cacggcccgc accgatccgg gaatgatcgt
    11281 gccgccgaat tcgtcgatca gcgaccggac gaaagggtcg gcatccacgt cctgctcggc
    11341 ctgtcgctgc agggcctcgc tgcgggcctg ggcgacggca tgcgcggtgg cgtctccagt
    11401 ggcgccgcat tcgatgtcga gttgcaccgc tgtgccgaaa tgttcggaca gcacggtgcg
    11461 caagcgcgcc ctgccatgcg tttcgaccag ggaccgcacg gcgacgcgca acgcgatgcg
    11521 atggtcgtcg gcttccagcc attcgctttg gcgcgccagc tccgcggcca gcccggtgag
    11581 cggcagcgaa gccgccagcg ccggccaggc gtccggggtc atggcccgca gactgggcga
    11641 ggcttgttca gcctggctcc tgccaggcat ggcgcccatc gcgcgttcga catcgggtgc
    11701 cagggtttcg aattcgtcgg ggtcgtcgga aggcgtgaag cccgtgtcat cgggagcgaa
    11761 ccccgcgtcc tcgtaccagc tttcgggagg cccgtcgtct ggcgcggtgt ccgatggctg
    11821 atcgttgcct gatggcggag agccgtcagt tgtggctcgg gaaatagtcg attgcgcgac
    11881 gggagcgggc ccgcggtcat ccaatcgggc gtccgaacca acggagccac caatgccgga
    11941 gacaacgatg ccgggctctg aaacgccagg aaattccggc gggcggatcg acgtgggtga
    12001 ttccacggct gcgtcaacgg cgccgggtgg atccgcaaca acgggcacag ccaaatcaga
    12061 tggagccagc tttgacgagg gggttcgaga tgacacgccg ctggc
//
LOCUS       DASTNK010000179        10593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1284778, whole genome shotgun sequence.
ACCESSION   DASTNK010000179 DASTNK010000000
VERSION     DASTNK010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10593
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284778"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VFR20_12970"
     CDS             complement(<1..716)
                     /locus_tag="VFR20_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU0231474.1"
                     /translation="MDSNSHAARQEPHPLFNQSLEKGLAVIRAFSAAHRTMTIAEVAA
                     QVGINKSSAQRMVYTLERLGYLKKHSRTQRYHLTVRALQLGFNYLATQTLIDVANPFL
                     SELSNFSQETVCLTEPDALEMVYIARFVSARYIPIHMPIGSRIPLYCTGSGRAYLSAL
                     PEDEAIALLRAGPRVAHTMYTRMDEGELLELLRNARRQGYAVNEQELFLGDTTLAAPI
                     IDGNGQPVAAVHIVAPSSRWA"
     gene            866..1630
                     /locus_tag="VFR20_12975"
     CDS             866..1630
                     /locus_tag="VFR20_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0231475.1"
                     /translation="MLRLNTCLRVVVAGACLSASLLGGNASAATLRVGATSTGIPFTF
                     LDVKTNTIEGMMVDTAKAVAKAAGDDVKIQQTVFSALIPSLTSDKIDVISAAMIKTPE
                     RAKVVAFTDPVYTHYGDGLLVRADDKHDYTSLDQLKGKVMGVQAGTTYYNLLMKKGYF
                     KAIRTYDSIADMARDLALGRIDAGIGDKPIMAYQIKQGTLKKVKLEPHYKQEAVGSIC
                     LVVRKGDTALLNKLNAAIAKVKASGELGKITAKWGI"
     gene            1741..2403
                     /gene="ehuD"
                     /locus_tag="VFR20_12980"
     CDS             1741..2403
                     /gene="ehuD"
                     /locus_tag="VFR20_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0033308 - hydroxyectoine transport
                     [Evidence IEA];
                     GO_process: GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="HEU0231476.1"
                     /translation="MHEFFVQAREFLPILLQGAVVTLQVTVLSFALSSVIGLALALLR
                     LSHVAPLRVGASSVINVIRGLPIIVQLFYIYFVLPDFGIQLTAFQAGVIGLGIAYSAY
                     QAENFRAGIEAVDDGQREAAQAIGMRTPLMMRRVVLPQAFRIALPPYGNTLVMMLKDS
                     SLVSTITVAEMTREGQLIASSTFQNMTVYTLVALLYLLMSLPLVWLLKRLERRMGGGR
                     KR"
     gene            2400..3155
                     /locus_tag="VFR20_12985"
     CDS             2400..3155
                     /locus_tag="VFR20_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU0231477.1"
                     /translation="MIEISGLRKSFGDHEVLRGVSLEVARGEVVCLIGPSGSGKSTVL
                     RCINGLESYDGGEVRVFGERVDRKSDAIHRLRGSLGMVFQRFNLFGHRTVLENVMEGP
                     VYVKRESVAKARGEALALLRKVGLEEKASAYPAQLSGGQQQRVAIARALAMRPEAMLF
                     DEPTSALDPELVGDVLQVMRALADEGMTMIVVTHEMGFAREVADRVCFLYDGCIAEEG
                     PADQVLGAPRNPRTQDFLRRVLHAATGNRPAGL"
     gene            3165..4481
                     /locus_tag="VFR20_12990"
     CDS             3165..4481
                     /locus_tag="VFR20_12990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU0231478.1"
                     /translation="MAGVRSIDSHPAAPSVWAATAPPAPETPPLQGAASTDVLVVGGG
                     FTGLSTALHLARAGTAVCVLEAHVPGWGASGRNGGQVNPSLKHDPDELVRMLGDRAEP
                     LIHAIGGSADLVFGLIEQHGIDCHPVRGGWMQVSYHDAAVPALHRRAEQWRRWGAPVA
                     MMDRGEVARRTGSGMFRGGWLDARAGAVQPLAYTRGLARAALEAGAGVHGDSPVVRLE
                     RRRGRWLASTAGGATVSADQVVLATNGYSDGLWPGLARTMLAANSFIAATRPLGAEAD
                     AILAGGETLSTAQRLLLYLRKDHQGRLLMGGRGLFSDPRGAGDFAHLERSLALLYPNL
                     GPFEFEYRWGGRIAITRDFLPHVHQPAPGVTVALGYNGRGVAAATAMGRHVAGLLAAG
                     DEGRRAFPFPITGMSPIPLHGLQRFYISAGVAWYGLLDRMEAGRRK"
     gene            complement(4500..4610)
                     /locus_tag="VFR20_12995"
     CDS             complement(4500..4610)
                     /locus_tag="VFR20_12995"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEU0231479.1"
                     /translation="MTHVDADVMEHLRESGKGWQTRVNAVLREAVEKGRL"
     gene            4989..5525
                     /locus_tag="VFR20_13000"
     CDS             4989..5525
                     /locus_tag="VFR20_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231480.1"
                     /translation="MGQKILRDGGHLEPSVQQWDWLGHGIYFWEGNPGRAMAWAVQRQ
                     KEGKIEAPFVLGAIIDLRHCLDLFDHDGLAQVKQAHRLLMQVSVAAGTKVPRNVGATA
                     DRAGRGLDCAVMNTLHQYRKERQELAYDSVRGPFLEGEEIYPGAGFRSENHIQLSVRN
                     AECVKGYFRPLHSKAASF"
     gene            complement(5711..5962)
                     /locus_tag="VFR20_13005"
     CDS             complement(5711..5962)
                     /locus_tag="VFR20_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU0231481.1"
                     /translation="MQRLRDFLRPKTPDAARRVGEAIRKGVQVLGSHPRMGRLVENLP
                     EQYREWPIDFGDSGYVARYRIDADMVIILAIRHQKETGY"
     gene            complement(5989..6267)
                     /locus_tag="VFR20_13010"
     CDS             complement(5989..6267)
                     /locus_tag="VFR20_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011408759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HEU0231482.1"
                     /translation="MTTATSIKLDDELKGRVRHLAEARQRTSHWIMREAIAQYVEREE
                     KRESLKHEVLQAWEEFQATGLHATAEEVDKWLASWGAETELLAPVCHK"
     gene            complement(6395..6688)
                     /locus_tag="VFR20_13015"
     CDS             complement(6395..6688)
                     /locus_tag="VFR20_13015"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEU0231483.1"
                     /translation="MSKHNTTNWEKVKAEADKPVTHDPETDLYDPNDEKAVIEFWKAA
                     KVSRPGRPRVAVKRPTLNMRVDADVMEHLRESGKGWQTRVNAVLREAVEKGRL"
     gene            complement(6666..6944)
                     /locus_tag="VFR20_13020"
     CDS             complement(6666..6944)
                     /locus_tag="VFR20_13020"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEU0231484.1"
                     /translation="MRLEFDPAKDAVNIAKHGISLADAMLVYESPNKLTLQSARLGEE
                     RLMDVAMVEVAGVVLVLVYVERTNVVRAISLRRASRAERRLYEQAQHD"
     gene            7173..7412
                     /locus_tag="VFR20_13025"
     CDS             7173..7412
                     /locus_tag="VFR20_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006403071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stability protein"
                     /protein_id="HEU0231485.1"
                     /translation="MSAVTIRNLPDETHRALKVRAAQHGRSTEAEIRDILIHATQPAG
                     RVKLGSLLARIGQEAGGFDLVIDRDPTSTPPVSFE"
     gene            7409..7831
                     /locus_tag="VFR20_13030"
     CDS             7409..7831
                     /locus_tag="VFR20_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU0231486.1"
                     /translation="MILLDTNVISEPLKARPDPRVVAWIDAQLVETLYIATISLAELR
                     YGIAVHPDGQKKAAVLENLEQLMLPLFRGRVLAFDEAAAAAYAQLRAAAARQGKAIGD
                     SDGYIAATARANGLAVATRDTASFLAAGVKVINPWEAP"
     gene            7862..8218
                     /locus_tag="VFR20_13035"
     CDS             7862..8218
                     /locus_tag="VFR20_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HEU0231487.1"
                     /translation="MNNRSLAHLPTISIVGTMANMAAELIADFKDVFDSGIIQMVIWK
                     VPTPVPPTTHGFKYRLVYVVNGRRVVGFDNERGKGDHCHLDGVELPYEFTSIETLIED
                     FIREVDKRRRTPWNAS"
     gene            8203..8571
                     /locus_tag="VFR20_13040"
     CDS             8203..8571
                     /locus_tag="VFR20_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231488.1"
                     /translation="MERKLTITIRREWKAALRDAGAVAEKGLREGVYQGETLNFESPG
                     AFFSRLTANRWAMLGELQGAGGVGVRELARRLYRDVKRVHEDAAVLVELGLLERTESG
                     ALVCPYDDIHVDMHLGRWAA"
     gene            complement(8586..8792)
                     /locus_tag="VFR20_13045"
     CDS             complement(8586..8792)
                     /locus_tag="VFR20_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231489.1"
                     /translation="MDIHPLCTEADYRAALREVSAYFNEQPKPSTPQGDRFEVLLTLV
                     EAYEARHYPIELNPDESLIRPTKP"
     gene            complement(8822..9091)
                     /locus_tag="VFR20_13050"
     CDS             complement(8822..9091)
                     /locus_tag="VFR20_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEU0231490.1"
                     /translation="MNGNKHATPTAWADPDDAPELTDEFFKHADEYHGNRLIRRGRPR
                     IENPKQALTVRYDTDVIAAFKAGGPGWQTRMNDALREWLHGREKA"
     gene            complement(9051..9323)
                     /locus_tag="VFR20_13055"
     CDS             complement(9051..9323)
                     /locus_tag="VFR20_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEU0231491.1"
                     /translation="MQIEFDAHKRDKTLIERGLDFARANEVFTGRHLTTQDTRGDDGE
                     PRYITVGNLDERIVVMAWAPRGEARRIISMRKANEREQARYANRLG"
     gene            9583..9807
                     /locus_tag="VFR20_13060"
     CDS             9583..9807
                     /locus_tag="VFR20_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231492.1"
                     /translation="MRNLIVLMPRVAHLRVYDNSRQADVGAPIEEPVLLLEMAQGRRL
                     YPATLADAATTPDWAKPALEAAFELDPLGL"
     gene            9997..>10593
                     /locus_tag="VFR20_13065"
     CDS             9997..>10593
                     /locus_tag="VFR20_13065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231493.1"
                     /translation="MKSKWQVLLLGLAWAASGGVAMAEVLEGEAVRRRALSDDVIVWP
                     PPNSAELVMDEARWGSPDAPFVFEGGVLHVGASFTSTRPFIVNEQAVINTHGDAVLTL
                     EGPVSAQPGSRQGLAKLGAGTLRLAGRNTYDGNTLLLQGGLDVLNGNALGAPYRTLNV
                     NTGTRLRYAPGVVVGNSIQLQADAIAGQVPAGSYEVITP"
BASE COUNT         1878 a         2918 c         3570 g         2227 t
ORIGIN      
        1 gcccaccgac tgctgggcgc gacgatatgt accgccgcca ccggctgtcc gttcccgtcg
       61 atgatgggcg cggccaaggt ggtatcccct aaaaacagct cctgctcgtt cacggcatag
      121 ccctgccgcc gcgcattccg cagcaattcc agcagttccc cttcgtccat acgcgtatac
      181 atggtgtgag ccacgcgtgg cccggcgcgc agcagcgcga tggcctcatc ttccggcaac
      241 gcactcaaat acgctcgccc agaccccgtg caatacaacg gaatccggct gcctatcggc
      301 atgtggatag ggatataccg cgcgctcacg aaacgtgcga tgtacaccat ttccagcgca
      361 tctggctcgg tcaggcacac ggtttcctgg ctgaaattcg acagttccga caggaagggg
      421 ttggccacat cgataagggt ctgggtggcc aggtagttga accctagttg cagggcgcgc
      481 acggtcaggt ggtagcgctg ggtgcgcgaa tgcttcttca ggtagcccag gcgctccagg
      541 gtgtagacca tgcgctgggc cgagcttttg ttgatgccga cttgcgcggc gacttcggcg
      601 atggtcatgg tacggtgcgc ggcgctgaag gcgcggatga cggccaggcc cttttccagt
      661 gactggttga acaaggggtg gggttcttgg cgcgcggcgt ggctgttgga gtccatgacg
      721 atagtataga aaagtttctt gaaaaatcga atatcgattc gttagattcg aataatggta
      781 aacacttatg gaataacgat tcgttcgact ctacgatttt aaggcttgcc cgcgatgcac
      841 ccgggcccac acaggagaac gcgtcatgct acgtctgaac acatgtcttc gagttgtcgt
      901 cgccggggct tgtttgtccg cctcgttgct gggggggaat gccagcgccg caacgctgcg
      961 ggtgggggcc acgtccaccg gcattccatt cacgttcctg gatgtgaaaa ccaacaccat
     1021 cgagggcatg atggtggaca cggccaaggc ggtggccaag gcggcggggg atgacgtcaa
     1081 gatccagcaa acggtgttct cggcgctgat tccgtcgctc acgtcggaca agatcgatgt
     1141 gatctcggcg gcgatgatca agacgccgga gcgcgccaag gtggtggcgt tcaccgatcc
     1201 ggtctacacg cattatggcg acggcctgct ggtgcgggcc gatgacaagc acgactacac
     1261 ctcgctggac cagctcaagg gcaaggtcat gggggtgcag gcgggcacga cgtactacaa
     1321 cctgcttatg aagaagggct acttcaaggc catccgcacg tatgactcca tcgccgacat
     1381 ggcgcgcgac ctggcgctgg gccgcattga cgcgggtatt ggcgacaagc ccatcatggc
     1441 ctaccagatc aagcagggca ccttgaagaa ggtgaagctc gagccgcact acaagcagga
     1501 agcggtgggc agcatttgcc tggtggtgcg caagggtgac acggcactgc tgaataaatt
     1561 gaatgcggcc atcgccaagg tgaaggccag tggtgagctc ggcaagatca cggcgaagtg
     1621 ggggatctga tggccgaccg ccaggttgac caccatcgcc gcgcgtggga tgtggcgtgc
     1681 cgtggtgtga cgcggtgatc gggcgccacg gccgcatgac gaaagaaagc gtgatcgtcc
     1741 atgcatgagt tttttgtaca agcccgcgag ttcctgccca ttctgttgca aggggccgtg
     1801 gtcacgctgc aggtgacggt gctgtcgttc gcgctcagca gcgtcatcgg tctggcgctg
     1861 gcgctgcttc ggctcagcca cgtggcgccg ctgcgcgtgg gcgcgtcgtc ggtcatcaac
     1921 gtgatccgcg ggctgcccat catcgtgcag ctgttctaca tctatttcgt gctgccggat
     1981 tttggtatcc agctcacggc gttccaggcg ggggtgatcg gcctgggcat cgcctatagc
     2041 gcgtaccagg cggagaactt ccgcgcgggc atcgaggcgg tggacgatgg ccagcgcgag
     2101 gcggcgcagg ccatcggcat gcgcacgccg ctgatgatgc ggcgtgtggt gctgccgcag
     2161 gcgtttcgca tcgcgttgcc gccgtatggc aacacgctgg tcatgatgct gaaggattcg
     2221 tcattggtgt ccaccatcac ggtggccgag atgacgcgcg aggggcagct cattgcctcg
     2281 tccacgttcc agaacatgac ggtctacacg ctggtggcgc tgctctacct gttgatgagc
     2341 ctgccactgg tctggctgtt gaagcggctc gagcgccgca tgggcggggg gcgcaagcga
     2401 tgatcgaaat ctcgggcctg cgcaagtcgt tcggcgatca cgaggtgttg cgcggtgtgt
     2461 cgctggaagt ggcgcgcggc gaggtggtgt gcctgatcgg gccgtccggg tcggggaagt
     2521 ccacggtctt gcgctgcatc aacgggctgg aatcgtacga cggcggcgag gtgcgcgtgt
     2581 tcggcgagcg ggtggaccgg aagtcggacg ccatccatcg cctgcgcggg tcgctgggca
     2641 tggtgttcca gcgtttcaac ctgtttggcc accgcaccgt gctggaaaac gtcatggaag
     2701 ggccggtcta cgtcaagcgc gagtccgtcg ccaaggcgcg cggggaagcg ctggccttgc
     2761 tgaggaaggt ggggctcgag gaaaaggcct cggcgtatcc ggcccagttg tcgggtgggc
     2821 agcagcagcg cgtggcgatt gcgcgcgcgc tggccatgcg gcccgaggcc atgttgttcg
     2881 acgagcccac ctcggcgctg gaccctgaac tggtggggga tgtgctgcag gtgatgcgcg
     2941 ccctggccga cgagggcatg accatgatcg tggtgacgca tgaaatgggg ttcgcgcgtg
     3001 aagtggcgga ccgggtctgt tttctgtacg acgggtgcat tgccgaggaa ggcccggcgg
     3061 atcaggtgct gggggcgccg cggaaccccc gcacgcagga ctttctgcgc cgggtgctgc
     3121 atgccgccac gggaaaccgg ccagcgggtt tataaggggg tgtgatggca ggggtgcggt
     3181 ccattgattc tcatccggcg gcgccgtcgg tctgggcggc caccgcgcca cccgcgcccg
     3241 agacgccgcc tttgcaaggg gcagccagca cggatgtgct ggtggtcggt ggggggttca
     3301 cggggttgtc gacggcgctg cacctggcgc gggcgggcac ggcggtgtgt gtgctcgagg
     3361 cgcatgtgcc gggctggggg gcgtcggggc gcaacggcgg gcaggtgaat ccgtcgttga
     3421 agcatgatcc ggacgaactg gtgcgtatgc tgggcgatcg cgccgagccg ctgatccacg
     3481 ccattggcgg gtcggccgac ctggtgttcg ggctgattga acagcatggc atcgattgcc
     3541 atccggtgcg cggggggtgg atgcaggtca gctaccacga cgcggcggtg ccggcgctgc
     3601 atcgccgcgc ggagcagtgg cggcgctggg gggcgccggt ggcgatgatg gaccgcggcg
     3661 aggtggcgcg gcgcacaggt tcgggcatgt tccggggcgg ctggctggat gcgcgggccg
     3721 gtgcggtgca gcccctggcc tatacacgcg ggttggcgcg ggcagccctg gaggcggggg
     3781 cgggagtcca tggggacagc ccggtggtgc gcctggagcg ccggcgcggt cgctggctgg
     3841 cgagcacggc gggtggcgcg acggtgtcgg ccgaccaggt agtgctggcc accaacggct
     3901 acagcgatgg cttgtggccg gggctggcgc ggacgatgct ggcggccaac agttttattg
     3961 ccgccacgcg gccgctgggt gccgaggccg atgcgatatt ggcgggcggc gagaccttgt
     4021 ccaccgcgca gcgcctgttg ctgtatttgc gcaaggatca ccaggggcgg ttgctgatgg
     4081 gcgggcgcgg gctgttttcc gatccgcgcg gcgccggcga tttcgctcat ctggaacgct
     4141 cgctggcgct gttgtacccg aacctggggc cgttcgaatt cgaataccgc tggggcgggc
     4201 gcatcgccat cacgcgcgat ttcctgccgc acgtgcatca gccggcgccg ggggtgaccg
     4261 tggcgctggg ctacaacggc cggggcgtgg cggccgccac cgccatgggg cggcatgtgg
     4321 cggggctgct ggccgccggc gacgagggcc ggcgcgcgtt tccgttcccc attactggga
     4381 tgtcgcccat tccgttgcat ggactgcagc ggttctatat ttcggcgggg gtggcgtggt
     4441 atgggttgct ggacaggatg gaggcgggga ggcggaagta ggggggaacg aggtggctct
     4501 catagccgtc ccttctccac cgcttcacgc aagaccgcat tcacccgtgt ttgccaacct
     4561 ttgccggatt cacgtaggtg ttccatcacg tcggcgtcga cgtgtgtgat ctatttgatc
     4621 atgattgcgg ataggcttgt attcggtgtg atagcatcaa gcttgccagt caaggatgcc
     4681 atcatgacga cgaaagttgc gactaaagcc tcaagaaaac ccacagccag ggcggtgaag
     4741 caatcacccc ggttagggaa ggctgtgcgc gtcatgacca ttcaagagcg cgtcgccgac
     4801 atgcaggctt atgggcggga gcaagctagc agcaggacat ccgcgctgtc cttcctgcgg
     4861 cgggctggta tcgtcacacc gaaaggcaac ttggctaagc cctttcgtga cgcttgatgc
     4921 cagtcgcttg gtctacctac gagtaccaac ccagcttcgt actcggattt cacggttgcg
     4981 ataaaacggt gggccagaag attctacggg acggaggcca cctggaacca tccgttcaac
     5041 agtgggattg gctcgggcac ggtatctatt tctgggaagg gaatccgggt cgtgcgatgg
     5101 cttgggctgt tcaacgccag aaggaaggca agatcgaggc tccctttgtc ttgggggcca
     5161 taatcgactt gcgacattgt ctggacctgt tcgatcatga tgggttggca caggtcaagc
     5221 aggcccaccg gttgctcatg caagtctctg ttgccgccgg cacgaaggtg ccgaggaatg
     5281 taggcgcaac ggctgacagg gctggccggg ggctggactg tgccgtaatg aacactctgc
     5341 atcagtatcg aaaagaacgc caggaactgg cttatgattc ggtgcgtggc ccctttcttg
     5401 aaggggaaga gatttacccc ggcgcgggtt tccgcagcga aaatcatatc cagctatctg
     5461 tgcgcaatgc agagtgcgtc aagggttatt tccgccctct gcacagtaag gccgcgtcct
     5521 tttagaacgc atgccgctcg ctcggctggg gtggcgtggt acgggttgct ggataggatg
     5581 gaggcgggga ggcggcgtta agcttttccc aggcagctcg gggcgattct cgattatctc
     5641 tttttatctt gattcggata aaacatttga atgaagataa cgaaggataa tcatgatcac
     5701 ctggttgtat tcaatacccc gtttcttttt ggtgccgtat cgccaggatg atcaccatgt
     5761 cggcatcgat gcggtatcgg gccacgtagc cgctatcgcc gaaatcgatc ggccattccc
     5821 ggtattgctc gggcagattt tcgaccagcc ggcccattcg cggatgtgaa ccaagcactt
     5881 gcacgccctt ccggatcgct tcgcccaccc gtcgtgcggc gtctggggtc ttgggacgca
     5941 ggaagtcacg cagtcgttgc atatccccga tcgccgccgg ggcgaatatt atttgtggca
     6001 tacaggggcc agtagttcgg tttcagcccc ccagcttgcc agccatttat cgacttcttc
     6061 ggcggttgcg tgcaggcctg ttgcctggaa ttcttcccat gcctggagta cttcgtgctt
     6121 caaggactcg cgtttttcct cgcgctcgac atactgtgcg atggcctcgc gcatgatcca
     6181 gtgcgaagtg cgctggcgtg cttcagccag gtgccgcaca cgccccttca attcgtcatc
     6241 gagcttgatg gatgttgccg ttgtcatgtg agggcctctg tagcgtaagg tattacttca
     6301 aactactata ctgctctgtc gttttgcagt ctatgcgggt tgggacttct caatcggcat
     6361 ctggcggggt tgttggccgg aaatgaagtg gctcttataa tcgccctttc tccacagctt
     6421 cacgcaagac tgcattcacc cgtgtttgcc agcccttgcc ggattcacgt aggtgttcca
     6481 tcacgtcggc gtcgacccgc atgttcaggg ttggccgttt cacggcgacg cgcggccgcc
     6541 caggccgcga caccttcgcg gccttccaga actcaatcac tgccttttcg tcgttcgggt
     6601 cgtagagatc ggtctcgggg tcatgggtaa ccggcttgtc ggcctcggcc ttcacctttt
     6661 cccagttagt cgtgttgtgc ttgctcataa agcctccgtt ctgctctgga tgcacgacgc
     6721 agggaaattg cccgaacaac gttggtgcgt tccacataga ccaaaaccag aacgaccccg
     6781 gcaacctcca ccattgccac atccatcaat cgctcttcac ccaagcgcgc tgattgcagg
     6841 gtcagcttgt tgggtgactc gtacaccagc atcgcgtccg caagggaaat gccgtgtttg
     6901 gcgatgttga cggcatcctt ggcgggatcg aattccagtc tcacgcaatt attgtattgt
     6961 cattaataaa ttgttgtcaa tacaataatt gatgatccgg gtcggttggg ctaagttcga
     7021 attgatagca aacgtaggtc tgagcgctgc tggccttcta gttgatgggc gcgtagcgcc
     7081 acaggaactg gttcaacgct gcttggccga agtcgataga tatcaaaaaa aattaatgat
     7141 atcattttaa gcaaagatat ggagtgatat caatgtctgc cgtgaccatt cgaaatctgc
     7201 ctgatgaaac acatcgcgct ttgaaggtgc gcgccgccca gcacggccgc agcaccgagg
     7261 ccgagattcg cgacatcctg atccacgcca cccaacccgc cggccgcgtc aagctcggct
     7321 cgttgctggc acggatcggt caagaggcgg gtggctttga tctggtgatc gaccgcgacc
     7381 ccacatcgac tccaccagtc agtttcgaat gattctgctc gataccaacg tcatttccga
     7441 gccgctcaag gcgcggccgg atccgcgcgt ggtcgcctgg atcgatgcgc aactggtcga
     7501 gacgctctac atcgcgacaa tttccctcgc ggaactgcgc tatggcattg ccgtgcatcc
     7561 cgacgggcag aaaaaggcgg cggtgctgga aaacctggag caactgatgc ttcccctgtt
     7621 ccgtggtcgc gtgttggcat tcgatgaggc ggctgcggcg gcctatgctc agctgcgggc
     7681 tgcggcggcg aggcaaggaa aggccatcgg ggattccgac ggctatatcg cggcgaccgc
     7741 cagggccaac gggctggctg tggcaacgcg ggataccgcg tcttttctgg ccgctggagt
     7801 caaggtcatc aatccttggg aggcgccttg atgggcaata tcaccgcccg tcggggtggg
     7861 cttgaacaat cgttcgttgg cgcacttgcc aacaatcagt atcgttggca caatggccaa
     7921 catggcggcc gagctcatcg ccgatttcaa agatgttttc gacagtggca tcatccagat
     7981 ggtgatttgg aaggtgccca cacccgtgcc accgacgacg cacggtttca aataccgatt
     8041 ggtttacgtt gttaatggtc gcagggtggt tgggttcgac aatgagcgtg gcaaaggcga
     8101 tcactgccat ctggatgggg ttgagctgcc ttatgaattc accagtatcg agacgctgat
     8161 tgaagacttc attcgggaag tcgacaagcg acggaggaca ccatggaacg caagctgaca
     8221 atcaccatcc gccgcgagtg gaaagcggcg ttgcgtgacg cgggcgccgt ggctgaaaaa
     8281 gggctgcggg aaggcgtgta ccagggggaa acgttgaatt tcgaatcgcc tggcgctttt
     8341 ttttcgcggc tgacggcgaa ccgctgggcc atgcttggcg agttgcaggg cgctggcggc
     8401 gtgggcgtgc gcgagttggc gcgccgtctg taccgggacg tgaagcgtgt ccatgaagac
     8461 gctgcggtgc tggtcgagct cggcttgctt gagcgtacgg aatccggcgc cttggtttgc
     8521 ccctatgacg acattcacgt tgatatgcat ctgggccgat gggcggcgta gggagccaat
     8581 cttccctatg gcttcgttgg tcggatcaag ctttcgtcgg gattgagctc gatcgggtaa
     8641 tgtctggcct cgtaagcctc cacaagcgtg agcagcacct cgaagcgatc accctgaggg
     8701 gtgctgggct tgggttgttc gttgaagtag gccgatactt cccgcaaggc ggcgcggtag
     8761 tcggcctctg tgcacaaggg gtgaatgtcc atgaatcaga gctccagatc aactgtttgc
     8821 gctacgcctt ttccctgcca tgcagccact cgcgtagcgc atcgttcatg cgtgtctgcc
     8881 agcccgggcc accggccttg aaggccgcga tgacatcggt gtcgtagcgc acagtaaggg
     8941 cttgcttggg gttctcgatc ctggggcgtc cgcggcggat gagccggtta ccgtgatatt
     9001 cgtccgcgtg tttgaagaac tcgtcggtca gctcgggggc gtcgtccggg tcagcccagg
     9061 cggttggcgt agcgtgcttg ttcccgttca ttggctttcc tcatgctgat gatgcggcgt
     9121 gcttcgccac gaggcgccca ggccatgacc actatccgtt cgtcgagatt cccgactgtg
     9181 atgtagcgcg gctcgccatc gtcgccccgg gtgtcctggg tagtgagatg ccgccctgtg
     9241 aagacctcat ttgcccgagc gaaatccagg ccgcgttcaa tcagggtttt atcgcgtttg
     9301 tgagcgtcga attcaatctg aatagtatta attgtagtta cattaattta tgtcgtcaat
     9361 acctcagtat ggcgagggat tcccactggc cgagcagcat gggcagagtt gttccatcgc
     9421 ggaatgtacg gtgcctggcc gaatcccctg tggggcgcgg gtggggtaga cgtgcgtgcc
     9481 catgccgcgc cgcctgaatg atgcgttggc cgccgtgcgc gcgttcggcg gggcggacac
     9541 gacattccag aagagaaaat tcgtgagcgc tgggtgtctt cgatgcggaa cctgattgtg
     9601 ttgatgccga gggtggcgca cttgcgcgtt tatgacaaca gccggcaggc tgatgtcggc
     9661 gcgcccattg aagaacctgt gctgctgctg gaaatggcgc aggggcgccg cctgtatccc
     9721 gcgacgctgg ccgatgccgc gacaacaccc gattgggcca aaccggcgct ggaggcggcg
     9781 ttcgagctgg atcctttggg tttgtgactt gagttattgg cgtccagcac caggcggaga
     9841 tcagtccgcg cgggcaatgg atcggtgagt gtgacgccac cacggcgacg tcacacttat
     9901 aaataaatat tacaaaaaca atcaatatgt acgatacttc ggtttgtccg catgtggttt
     9961 gtacgtacgg actttcacaa ccaaaggaag agacatatga aatcgaagtg gcaggtgttg
    10021 ttgttgggat tggcttgggc ggcgtcgggg ggtgtggcga tggccgaggt gttggagggc
    10081 gaagcggtgc ggcgccgggc gttgtcggac gatgtcatcg tctggccgcc gccgaatagt
    10141 gctgaactgg tcatggacga ggcgcgttgg ggctcacccg atgcgccttt tgtttttgag
    10201 ggcggcgtgt tgcacgtggg tgcgtcgttc acgtcgaccc gcccgttcat cgtgaatgag
    10261 caggcggtga tcaacacgca tggcgatgcg gtgttgacgc tggaagggcc ggtgtcggcg
    10321 cagccggggt cgcgccaggg gctggccaag ctgggcgcgg gcacgctgcg attggcgggg
    10381 cgcaatacgt atgacggcaa taccttgctg ctgcagggtg ggctggatgt gctgaatggc
    10441 aatgccctgg gggcgccgta ccgcaccttg aacgtgaaca cgggaacgcg gctgcgctat
    10501 gcgccgggcg tggtcgtggg caacagcatc cagttgcagg cggatgccat cgccggacag
    10561 gtgccggcgg ggtcgtatga ggtgatcacg ccg
//
LOCUS       DASTNK010000180         6300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1469858, whole genome shotgun sequence.
ACCESSION   DASTNK010000180 DASTNK010000000
VERSION     DASTNK010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6300
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469858"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VFR20_13070"
     CDS             complement(<1..974)
                     /locus_tag="VFR20_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0231494.1"
                     /translation="MGNNAGEYDKTYAAATADPTGFWANIAEDIHWTKRWNRVIDDSN
                     KPFYRWFTGGELNTCHNCVDRHVEEGRADQLALIYDSPLAGIIRRITYRELRDETARC
                     AGALRDLGVGKGDTVIIYMPMVAEAAIAMLACARIGAVHSVVFGGFAAPELAKRVADA
                     KPKVVMSASCGLLPGKAVPYKPLLDEALKIAHAEDTPCLILQRPELECDMIAGRDHDW
                     AGAVANAAPADCVTVAATDPLYIIYTSGTTGIPKGVVRDNGGHAVALKWSMHAVYGME
                     PGDVYWAASDVGWVVGHSYIVYAPLLQGCTTILYEGKPVGTPDAGAFWR"
     gene            1288..2304
                     /gene="pstS"
                     /locus_tag="VFR20_13075"
     CDS             1288..2304
                     /gene="pstS"
                     /locus_tag="VFR20_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU0231495.1"
                     /translation="MNRSKFSFLALAVALTGSVGTAHAVTITGAGSSFVYPVIAKWSA
                     AYAGKTGNQVNYQSIGSGGGITQIKAGTVDFGASDMPLDEATLDKFGLGQFPDVIGGI
                     VPTVHLQGVAPGKLVLSGPVLADIFLGQITSWSDPAIAALNPGVHLPDAKIVVVHRSD
                     GSGTTFNFTDYLSKVSPTWKAKVGEGVSVSWPTGIGGKGNEGVSAYIKQMPNSIGYVE
                     YAYVKQNHLGYTRMKNSAGQVVEPDTASFQAAAATADWAHAKNFNLLMTNAPGAKAWP
                     ITATSWVIMYKQPKNAGRSKVALDFFRHAYAEGKPAALALDYVPLPATLVRQIESYWK
                     ASFK"
     gene            complement(2691..2764)
                     /locus_tag="VFR20_13080"
     tRNA            complement(2691..2764)
                     /locus_tag="VFR20_13080"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2730..2732),aa:Cys,seq:gca)
     gene            3059..3637
                     /locus_tag="VFR20_13085"
     CDS             3059..3637
                     /locus_tag="VFR20_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEU0231496.1"
                     /translation="MRLCVFSGSNAGRRPAYQGAARLLGAGLAKAGIGVVYGGSSVGL
                     MGAMADAALAEGGEVIGIIPQSLVDKEMSHTGLSDLRVVGSMHERKAMMSQLSDGFVA
                     LPGGLGTFEELFEVWTWAQLGHHQKPCAVLNIEGFYDGLLSFLDRVVDEAFIKKQHRD
                     MLVVASNVDELLTALHKYQPPQVTKWIRSEEA"
     gene            complement(3684..4511)
                     /locus_tag="VFR20_13090"
     CDS             complement(3684..4511)
                     /locus_tag="VFR20_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEU0231497.1"
                     /translation="MAPSIQQKRATFKALHEQGCFVLPNPWDIGSALYLQAIGFKALA
                     TTSSGHAWSHGQPDGAMPRNAILAHLRAMVEATDLPINADFESGFATNPQGVAESVRM
                     AVATGVAGLSIEDSTGDPDTPLLPIDEAVARMRAARAAIDETGGDTLLVGRAENFFAG
                     RPDLNDAITRLKAYSEAGADCLYAPGIRTREQITAVVAAVAPKPVNLLVGSTSEFTMK
                     DIAAMGVRRVSVGGAMARAAWGGFQRAARALAEGGRFDGFADAAAGKDLNALFGRDA"
     gene            complement(4693..5109)
                     /locus_tag="VFR20_13095"
     CDS             complement(4693..5109)
                     /locus_tag="VFR20_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEU0231498.1"
                     /translation="MHASKFQHFLDSNVVLYLLSQDEAKADKAESLLKARPVISVQVL
                     NEVTHVCRRKLRMSWNDIAQFLELVRSFCKVIPLTEAIHDHARSIADRRTLSFYDACI
                     VAAAVSAGCQTLYSEDMNPGQVMDDGLSLRNPFAAA"
     gene            complement(5096..5329)
                     /locus_tag="VFR20_13100"
     CDS             complement(5096..5329)
                     /locus_tag="VFR20_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012366057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEU0231499.1"
                     /translation="MQVAKWGNSLAVRLPASLVEALELREGDDIEIVVDDPHTFAVRK
                     KPGTDALLKRLRAFRGKLPADFRFSRDEANARE"
     gene            complement(5479..6189)
                     /gene="queC"
                     /locus_tag="VFR20_13105"
     CDS             complement(5479..6189)
                     /gene="queC"
                     /locus_tag="VFR20_13105"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEU0231500.1"
                     /translation="MAGKHQRRALVLFSGGQDSTTCLAWALAHFGHVETIGFNYGQRH
                     RSELDCRITLRHRMAELWPDWAPHLGEDHLLDVNALTQLGTSAMTDEVAITMQADGLP
                     NTFVPGRNLLFFTLAAALAYRRGLDVLVGGMSQTDYSGYPDCRDNTLKALQVALSLGV
                     DRQLALETPLMWLDKADTWELARQLGGGALVDLIRTDTHTCYLDRRDTLHAWGYGCGH
                     CPACALRAQGYQEWVVRQ"
BASE COUNT         1190 a         1868 c         1952 g         1290 t
ORIGIN      
        1 cgccagaagg cgccggcatc gggcgtgccc actggcttgc cctcgtacag aatggtcgtg
       61 cagccctgca gcagtggtgc gtagacgatg taggagtggc ccaccaccca gcccacgtcc
      121 gatgccgccc aatagacgtc gccgggttcc atgccataca cggcgtgcat cgaccacttc
      181 agcgccacgg catggccgcc gttgtcgcgc accacccctt tcgggatgcc ggtcgtgccg
      241 gacgtataga tgatgtagag cgggtcggtg gcggcaacgg tgacgcagtc ggcaggggct
      301 gcgttggcga cggcccctgc ccagtcatgg tcccggcccg cgatcatgtc gcactcgagt
      361 tccgggcgtt gcagaatcag gcacggcgtg tcctcggcgt gtgcgatctt caaggcttcg
      421 tccagcagcg gcttgtaggg cacagccttc ccgggcagca gcccgcacga ggcggacatc
      481 acgactttgg gcttcgcgtc ggccacgcgc ttggccagct cgggtgccgc gaaaccaccg
      541 aagacgaccg agtgcacggc accgatgcgc gcgcaggcca gcatcgcgat cgcggcctca
      601 gccaccatcg gcatgtagat gatgacggta tcgccttttc ccacgcccag gtcgcgtagc
      661 gcaccggcgc accgggcggt ttcgtcgcgc agttcgcggt aggtgatcct gcgtatgatg
      721 ccggccagcg ggctgtcgta gatcaacgcg agttgatcgg cacggccttc ctcgacatgg
      781 cgatcgacgc agttgtggca ggtgttcagt tccccgcccg tgaaccagcg atagaagggt
      841 ttgttggaat cgtcgatgac cctgttccag cgcttcgtcc agtggatgtc ttcggcaatg
      901 tttgcccaga atccggttgg atcggccgtg gcggctgcat aagtcttgtc atactcgcca
      961 gcgttgttgc ccattacgtc tcctcgctat gtgccatgaa ttttattgtg gccgtgagcg
     1021 gccactattt tcgtacttta ctaccagagt gtgcggtgac ttgcggcagg ccgggccgaa
     1081 ctcatggata tcgccatccc gtccaatcgc cacaccagcc ggaagcatgg accatcctgt
     1141 tccggcatgg ctgccgccat ccagtgcctg gccagggctg tatgccgttg tcatgaaact
     1201 gccaaatttt cttcatgaaa cggtaacgcg cgggcgtttc aatacgtatc gtcatcaacg
     1261 acatccaaaa ggacgtaaat gatttccatg aatcgcagca aattcagttt cctcgctctg
     1321 gccgttgcgc tcacaggttc cgtcgggacc gcccatgcag tcaccatcac cggtgcgggc
     1381 tcgagctttg tctacccggt gatcgccaaa tggtcggccg cctatgcggg caagaccggc
     1441 aatcaggtta attatcagtc cattggttcg ggcggtggca tcacgcagat caaggccgga
     1501 accgtcgatt tcggcgccag cgacatgccg ctggacgaag ccacgctcga caagttcggc
     1561 ctgggtcagt tccccgacgt catcggtggc attgtgccca ccgtccatct gcagggcgtg
     1621 gctcccggca aactggtgtt gagcggtcct gtgctggccg acatcttcct gggccagatc
     1681 accagttggt ccgatccggc tatcgccgcc ttgaacccgg gcgttcatct gcctgacgcg
     1741 aagatcgtcg tggtgcaccg ctcggacggt tctggcacca ctttcaactt cactgactac
     1801 ctttccaaag tcagccccac gtggaaagcc aaggtgggcg agggcgtttc ggtgagctgg
     1861 cccacgggta taggcggcaa gggcaatgag ggtgtgtcgg catacatcaa gcaaatgccg
     1921 aactcgatcg gttatgtcga gtacgcttat gtgaagcaga accaccttgg atatacgcgc
     1981 atgaagaaca gcgccggcca agtggtggag ccggacaccg ccagtttcca ggccgcggcc
     2041 gcgaccgccg actgggcgca tgcgaagaac ttcaacctgc tgatgaccaa cgcgcccggc
     2101 gccaaggcgt ggccgatcac tgccacttcg tgggtgatca tgtataaaca gccgaagaat
     2161 gccgggcgtt cgaaggttgc gctggacttt ttccgccacg cctacgccga aggcaagcca
     2221 gccgccctgg ctttggatta cgtgccgtta cccgccacgc tggtgcggca gatcgagagc
     2281 tactggaaag cgtcgttcaa gtaacaggta ttgcggtctg gggggctgtc acccctcaga
     2341 tacttgttca ggtttgaact ggtccgaatg cgcgctccag gtcttcgatc aggcctgggc
     2401 cgctcggatc ccggttcagt gtctgccggg tccagccgct tcctctggtg ccgccaaggc
     2461 aaacagacag ccggaccaca gttgcatcat tccctgatgc tcagccagtt tttctcgcct
     2521 gctgagcccc ccatcaaata cagtccaccg ccatcgttaa ggcggtattt cctggctgtg
     2581 gtaacccttc aagtccgtgg cttagttgtt gatttttggc aattttgcca tgctggccaa
     2641 ggagtttttg aagggagaat caaggaggga agtacccgta aagggtattc tggaggcgca
     2701 actcggaatc gaaccggggt acacggattt gcaatccgct gcatgaccac tctgccattg
     2761 cgccttgcca acggagccaa acacagggcg tcagaaagga agccagctat tctacactat
     2821 ccgccacatt gcgggggtga gggcagtgat cgataacgga aggtggaaac gcttactgct
     2881 tgatgcggcg cgctggactc tacaatagcc gcgaggccag acagcgtttc gaatccagcc
     2941 catttcacaa tgagcggtcg attcggcgag tttgggtgtg gcaaacctgg tatgtctggt
     3001 gcgggccata ggacgcctcg accctcgcca ggcatcgaca tacagaagga ctcacgtcat
     3061 gcggttgtgc gttttttccg gctccaacgc ggggcgccgg cccgcctatc aaggggcggc
     3121 gcggctattg ggcgcagggc ttgccaaggc gggtattggc gtggtctacg gagggtcgtc
     3181 ggttgggctg atgggggcca tggccgacgc cgcgctggcg gagggtggcg aggtcatcgg
     3241 catcattccc cagtcgctgg tggacaagga aatgtcccac accggtctgt ccgatctgcg
     3301 tgtggtcggt tccatgcacg aacgcaaggc gatgatgtcg cagttgtccg acgggttcgt
     3361 ggcgctgccg ggtgggcttg gcacttttga agagctgttc gaggtttgga cctgggcgca
     3421 actgggccat caccaaaaac catgcgccgt gttgaatatc gaaggctttt acgatggcct
     3481 gttgtcattc ctggatcggg tcgtcgacga ggcgttcatc aagaagcagc atcgtgacat
     3541 gctggtggtg gcgtccaacg tggacgaatt gttgacggcg ttgcacaagt accagccgcc
     3601 ccaggtcacg aaatggataa ggtctgaaga ggcgtagcgc cgcgtgggtg gggtgctctt
     3661 cctttgacgt gccgggagcg ggatcaggca tctcgcccga acagcgcgtt caagtctttt
     3721 ccagccgccg catcggcgaa gccatcgaat cgcccaccct cggccagcgc gcgcgcggcg
     3781 cgctgaaaac cgccccacgc cgcgcgggcc atcgcgccgc cgacgctgac gcgccgtaca
     3841 cccatggcgg cgatgtcctt catggtgaat tcgctggtag acccaaccag tagattgacg
     3901 ggcttgggcg ccacggcggc cacgacggcg gtgatctgtt cgcgggtacg tataccgggc
     3961 gcgtagaggc agtccgcacc ggcttcagag taggctttca ggcgggtgat ggcgtcgttc
     4021 agatcggggc gccccgcaaa gaagttctcg gcccgtccga ccagcaaagt gtcgccgcca
     4081 gtctcgtcga tggcggcacg cgcggcgcgc attctggcca cggcttcatc gatggggagc
     4141 aaaggcgtgt cgggatcgcc cgtggagtct tcgatcgaca ggcccgccac gccggtggcg
     4201 accgccatgc gcacgctttc cgcgacacct tgcggattag tggcgaagcc actttcgaaa
     4261 tccgcgttga tcggcaggtc ggtggcttcg accatggcgc gcaggtgcgc caatatggcg
     4321 ttgcgcggca tggcgccgtc gggttggccg tgggaccacg cgtgaccgga actggtggtg
     4381 gccagcgctt tgaaccctat tgcctgcaaa tacagcgcgc tgccgatgtc ccagggattg
     4441 ggcaatacga agcagccttg ttcatgcagc gccttgaagg tagcgcgttt ttgttgaatg
     4501 gagggggcca tggggttctc cggtttaaag ggcgtggctg ccattgtgct acgtcggtgg
     4561 cttacttgca cgtcaccagc caaccgttga attcatcgcg gttccgacag actcgtgtct
     4621 cccaatttcc ggtgtggaac accactcggc attcgaactg tggccaatgg cacgagcaac
     4681 ggtgctggtc gactatgcgg cggcgaacgg gtttctgaga ctcaagccat catccatgac
     4741 ttgtccggga ttcatatctt cactatagag tgtctggcaa cccgccgata cggcggcggc
     4801 aacgatgcag gcgtcgtaga acgatagggt gcgtcggtcg gcgatcgatc gggcatggtc
     4861 atggatggcc tcggtgagcg ggatgacctt gcaaaagctt cgtaccaatt ccaggaactg
     4921 tgcgatgtca ttccagctca tccgaagctt gcgccgacat acatgtgtca cttcattgag
     4981 cacctgcaca ctaatcacag gccttgcctt caacaggctt tccgctttat ctgctttggc
     5041 ctcgtcctgt gacagcaggt acaagaccac attgctgtcg agaaaatgct ggaatttact
     5101 cgcgtgcatt ggcttcatcc cgactgaaac ggaaatccgc cggcaatttt ccccggaagg
     5161 cgcgtaaccg cttcagcaac gcgtcggtcc cgggtttctt gcggacagcg aacgtgtgcg
     5221 gatcgtccac gacgatctcg atatcgtcac cttcacgcaa ttccagggcc tccaccaggc
     5281 tggcggggag gcgtaccgcc aagctgttac cccattttgc gacctgcatg tttgtgctcc
     5341 gtcgtttgat atacttttta agtgtatatc cgaaaaagaa aaacgcaagg tgcgacattc
     5401 atggcgtgtg tcatcgataa gagttcgcat atacgatcct cggtggcgtg aagctgcggg
     5461 ccgcgaacca cggaccaatc actgccgtac cacccattcc tggtaaccct gggcgcgcag
     5521 cgcgcaggcc gggcagtggc cgcagccata accccaggcg tgcaaggtgt cgcggcggtc
     5581 gagatagcaa gtgtgggtgt cggtccgtat caggtccacc agtgccccgc ctcccaattg
     5641 ccgcgccaat tcccacgtgt cggccttgtc gagccacatc aaaggggttt ccagcgccaa
     5701 ctgacgatcc acgcccaggc tcagcgccac ctgcaaagcc ttgagggtat tgtcgcgaca
     5761 gtccgggtag ccggagtaat ccgtttgtga cataccgccc acaagcacgt ccaatccacg
     5821 ccggtacgcc agcgcggcag ccagcgtgaa aaacagcaga ttgcggcctg gcacgaaggt
     5881 gtttggcagg ccgtcagcct gcatcgtgat ggcaacctcg tcggtcatgg ccgatgtgcc
     5941 cagttgcgtc agcgcattga cgtccagcag gtggtcttcg ccaagatgtg gcgcccagtc
     6001 tggccatagt tcggccatcc ggtggcgcaa cgtgatgcga cagtccagtt ccgatcgatg
     6061 gcgctggccg tagttgaagc cgatggtctc gacatggccg aaatgcgcca gcgcccaggc
     6121 aaggcaagtg gtggaatcct gtccgccgga gaacaggacc agggcgcggc gctggtgttt
     6181 cccggccatg atcatgcccc ggccagcctg atctcgtgcc gtttcatgaa ccggatgatg
     6241 gcgttcaggg cgatgccgta caagggcagg aacaacagca ggctgacagt cagtttgatg
//
LOCUS       DASTNK010000181        11263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_176142, whole genome shotgun sequence.
ACCESSION   DASTNK010000181 DASTNK010000000
VERSION     DASTNK010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11263
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176142"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(426..1151)
                     /locus_tag="VFR20_13110"
     CDS             complement(426..1151)
                     /locus_tag="VFR20_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HEU0231501.1"
                     /translation="MSALTTAIREQLEQVSVATLTTTLFKRGLRNQLIQGILPVCRTG
                     GKRKLIGPAYTLRYMPAREDLNKLEVFRDREHPQRKAIEECPPGHVMVIDSRKDARAA
                     SAGSILITRLQERGVAGVVTDGGFRDSPDIAQLDMPAYHLHPAPPTNLTHHQAIDINV
                     PIGCGDAPVFPGDIIFGDEEGVVVIPAHVVAECAQEAVEMTAFEDFVTEQVKAGRSVL
                     GLYPATDPKTPEQFAAWRKANNR"
     gene            complement(1163..2890)
                     /gene="araD"
                     /locus_tag="VFR20_13115"
     CDS             complement(1163..2890)
                     /gene="araD"
                     /locus_tag="VFR20_13115"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HEU0231502.1"
                     /translation="MKTPDTLRSARWFAPDDLRGFGHRSRFMQMGYGPDDWAGKPVIG
                     IINTWSDIQPCHAHFKQRVEDVKRGVLQAGGMPIELPALSLAEQYVKPTTMLYRNMLA
                     MDTEELIRCHPIDGVVLMGGCDKTTPALLMGATSAGVPAIYIPAGPMLRGNWEGKTLG
                     SGSDAWKFWDERRAGHINNEQWLGIEGGIARSHGHCMTMGTASTMTAIADALGMTLPG
                     ASSIPAADVNHIRMCAAVGRRAVEMVWEDLVPSRIQTVQAFRNAITVAMAMGCSTNAI
                     IHVIAQARRANCDISLDDFERASRQVPVIANVRPSGDKYLMEDFYYAGGVRALMSELK
                     PLLHLDALTVTGKTLGENLEGAKVFNTDVIRSLDNPIYAEGSLAVLKGNLAPDGCVIK
                     PAAADKRFLKHRGKALVFDSYPDMKARINQPDLDVTPDHVLILRNVGPLGGPGMPEWG
                     MIPIPQKLVEQGVRDMVRISDARMSGTSYGACILHVAPEAYIGGPLSLVETGDEIVVD
                     VAARSINMMVSDTELARRRAARPAPAPRYERGYGWMFSRHIQQADKGCDFDFLQTQFG
                     ATAGEPDIF"
     gene            complement(2905..3765)
                     /locus_tag="VFR20_13120"
     CDS             complement(2905..3765)
                     /locus_tag="VFR20_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0231503.1"
                     /translation="MGRATITLRQATPADDKKAVLVRQLSIWQKLSNNTFLRKTLIIV
                     ILALAWQIYGRAVDNNLLFPTLSDTIASFFSSIHSGVLLDRAWTSIRILLLGYVIGIV
                     IAAIITILAISTRIGTDFLEVMTAMFNPLPAIALLPLALLWFGLGTTSIVFVLLHSVV
                     WVVALNTQGGFLAVSQTLRMVGRNYELTGLSYVARILIPAAFPSILTGLKIAWAFAWR
                     TLIAAELVFGVASGKGGLGWYIFENRNLLNIPAVFAGLLTVIVIGLLVENFFFRLIEK
                     KTVRRWGMQS"
     gene            complement(3765..4532)
                     /locus_tag="VFR20_13125"
     CDS             complement(3765..4532)
                     /locus_tag="VFR20_13125"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0231504.1"
                     /translation="MSHLLEVDSVTLRYKTPDSIVTATDRVSFNVNSADRFVLLGPSG
                     CGKSTLLKAVGGYLNPEEGEIRIKGKPVRRPGADRMMVFQEFDQLLPWKTVLENVMFP
                     LTVARKMPRKQAEERARAYVEKVHLGNFADSYTHTLSGGMKQRVAIARGMCMEPDILL
                     MDEPFAALDALTRRQMQDELLQLWEGTRFTVMFVTHSIAEAIRVGNRILLLSPHPGEV
                     KAEVSDVESVSHEDGSAASLEKEIHEMLFNDTNMGHA"
     gene            complement(4549..5565)
                     /locus_tag="VFR20_13130"
     CDS             complement(4549..5565)
                     /locus_tag="VFR20_13130"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0231505.1"
                     /translation="MKFIPHIGLGAILCAALATAGVTAHAQEMHEITITKQPSIIYLP
                     ALVMEKQGLIAKAAAKEGIKGLKVNWRAFSSGGASTDALLSGNVQIVNSGVGNMLLLW
                     DRTKGKVKGITTNSALPLTLVTRNPKIHSLKDYGPKDKISVPTVRVSTQAILLQMACE
                     KVFGKDQWSKLDANTVQLGHPDAAAMLANPNGEITSAFGAPPYSYRELKTIPHAHIVL
                     RSTDIIQGGLSQSTLFTTTKFAEANPKIIQAVLTASKEAIDYIKSHTRESVEIYRELS
                     GDKTSVDELMGVLKQPGMMAYQMAPAGSMKFAQHMYKVGILKTLPQKWTDYFLPVSAS
                     LNGS"
     gene            5772..6458
                     /locus_tag="VFR20_13135"
     CDS             5772..6458
                     /locus_tag="VFR20_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0231506.1"
                     /translation="MASTLKLDRYRQAAPQLYELLRDKIVALEYLPGAVLSRVDISMQ
                     FNVSQTPVREALLRLAEERLVDVFPQAATRVSLIDTHIAEETHFLRRAIELEIVRELA
                     LHGAPSLIATLRDLVATQVRLRDRQDYDGFVVADREFHRSLYAAVGKEELWRLVCSRS
                     GHIDRLRRLHLPAGDKMARIVDDHGRILEAITGRDPGAAQDRLRQHLSGTLENMDRIR
                     DEHPQYFVQG"
     gene            complement(6508..8667)
                     /locus_tag="VFR20_13140"
     CDS             complement(6508..8667)
                     /locus_tag="VFR20_13140"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEU0231507.1"
                     /translation="MDKRIAHHRLQVADVLHQFIEHEALPGTGLDAGAFWSGLDALVH
                     DLTPRNRALLAERDRLQAELDKWYKQHPGRVADAGAYRAFLEKIGYLAPVPDHVAVKT
                     TQVDTEITHQAGPQLVVPITNARYALNAANARWGSLYDALYGTDALSEDGGATRAGTY
                     NPVRGAKVIAYARKFLDDNAPLAQGSHAQAKAYAVANNALRVTLLDGTVTGLADPALC
                     VGLRGQADAPEAIVFRHNGLHFELQIDKSSPIGQQDAAGVKDILMEAAITTIVDFEDS
                     IAAVDARDKVCAYRNWLGLMQGNLTEEVSKAGKTFTRALNPDRTYTDLQGKTQALHGR
                     ALLFVRNVGHLMTNPAILDENGHEVFEGILDAAVSSLIALHDLKRRGNSRTGSIYIVK
                     PKMHGPDEVAFADELFARVEALLNLPAHTIKLGIMDEERRTSANLKACIGRTAARVAF
                     INTGFLDRTGDEMHTSMHAGAMVRKGDMKRSTWIDAYERNNVQVGLQCGLRGQAQIGK
                     GMWAMPDLMAAMLEQKSAQLKAGGNTAWVPSPTAATLHALHYHQIDVQAVQRGMEGKS
                     EPLLDAILTVPVAASTDWSAEEIQQELDNNAQGILGYVVRWIDQGIGCSKVPDIHNVG
                     LMEDRATLRISSQHIANWLLHGVTTKEQVQDTLKRMAAVVDQQNAGDPAYRPMAPDFD
                     ASVAFKAASDLVFKGLEQPNGYTEPLLHHWRQVVKAQ"
     gene            complement(9145..10572)
                     /gene="pyk"
                     /locus_tag="VFR20_13145"
     CDS             complement(9145..10572)
                     /gene="pyk"
                     /locus_tag="VFR20_13145"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU0231508.1"
                     /translation="MKSRRARIVATLGPASSSPERIKALIEAGVDVFRLNFSHGTHAD
                     HAERLRIIREAEHAAGRPIGVLMDLQGPKLRVGNFKGGSVELEVGKPYRLDLDPTDGD
                     ARRATLPHPEIFAALEVGADLLINDGKLRLRVDRFGPDFADTTVMVGGTLSNHKGVNV
                     PGTVLPISPLTAKDRIDLDYGLSLGVDWVALSFVQRPEDVTEARAIIGNRAWIMVKLE
                     KPAAIDHLDAIIELADGVMVARGDLGVELPPQKVPMLQHQIVRKSRAAGRPVVVATQM
                     LESMISAPIPTRAEASDVATAIYSGADAVMLSAESASGEYPVEAVTIMERIIHEVEND
                     PAWRAGLDATHGESQPNTPDAICCALRRVASLLDPAATVAYTASGFSALRASRERPTT
                     PILALTPELATARRLTMAWGIHPLPFETQLDHVTDMIDHATSEAVATGLAHNGDQIIV
                     IAGLPFGRSGSTNLMHIATIQDEPL"
     gene            complement(10609..>11263)
                     /locus_tag="VFR20_13150"
     CDS             complement(10609..>11263)
                     /locus_tag="VFR20_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HEU0231509.1"
                     /translation="STCADALDIVRRYEITLPAAARHLLESGAGETIKPGDLRLARVE
                     THLIATPQMALTAAAEVAEQAGLRTLILGDSIEGEAREVGRVMAGIALQARHHAQPAR
                     PPCVLLSGGETTVTVRGQGRGGRNVEFLLSLALALNDTDGIHAVAGDTDGVDGLEEIA
                     GAFITPDTLARARARGLKARDHLDDNNGHGFFEALGDSLVTGPTLTNVNDFRAILVA"
BASE COUNT         1797 a         3259 c         3974 g         2233 t
ORIGIN      
        1 ggcaatatcc ctccatgcat gccgctgtcg tagccccagg gtgggggagg tggcgcaggc
       61 cggcgtttcg gccagcgccg gatctggctt gaccaggcgg gggcagtcgt cgggcggtgt
      121 ttgagcgcag cctcgcgata gcgaggcgta gcgagtttag cccgacgccc gcctggacga
      181 gacagacccg ggcagtcggg gcgcgtagcg ccacgaccgc tggacgcgcc ggcctgcgcc
      241 acctccccca ccctggggcg ggttaataga atgatccacc cagtggaata gaaccgcccc
      301 ggccagaacc gttcatatgg ctcaaacggt taaagcgact caaacggtta aagcgactca
      361 aacgattcaa acggctcaaa cagttcaaac cgtgacgtca cttgtgttca gcacaccccg
      421 ctttctcagc ggttgttggc cttgcgccag gcggcgaatt gttccggtgt tttcgggtcg
      481 gtggccgggt ataggcccag cacgctgcgg ccggctttga cctgctccgt gacgaagtct
      541 tcgaaggcgg tcatttcgac ggcttcctgg gcgcattcgg cgaccacgtg agccgggatg
      601 acgaccaccc cttcttcgtc accgaagatg atgtcacccg ggaagacggg ggcatcgccg
      661 cagccgatgg gcacgttgat gtctatggcc tgatggtgcg tgaggttggt cgggggcgcg
      721 ggatggaggt ggtaggccgg catgtcgagt tgcgcgatgt cgggcgagtc gcggaagccg
      781 ccgtcggtga cgacgccggc cacgccgcgt tcctggagtc gcgtgatgag gatgctgccg
      841 gcggaggccg cgcgggcgtc tttgcggctg tcgatgacca tgacgtggcc aggggggcat
      901 tcttcgatgg ctttgcgttg cgggtgttcg cggtcgcgga agacttcgag tttattgagg
      961 tcttcgcgcg cgggcatgta gcgcagtgta taggccggcc cgatgagctt gcgcttgccg
     1021 ccggtgcggc acacaggcag gatgccctga atgagctggt tgcgcaggcc gcgcttgaag
     1081 agtgtggtgg tgagggtggc cacgctcact tgttcgagct gttcgcggat ggcggttgtc
     1141 agtgccgaca tggggtgttc tcctagaaga tatctggttc gccggcggtg gcgccgaatt
     1201 gtgtttgcag gaagtcgaaa tcgcagccct tgtcggcctg ttgtatgtgg cggctgaaca
     1261 tccagccata gccgcgttcg tagcgtggcg ccggtgccgg gcgcgcggcg cggcggcggg
     1321 cgagttcggt gtcggacacc atcatgttga tggaccgggc agcgacgtcc acgacgattt
     1381 cgtcgccggt ttccaccagg gacagcgggc cgccgatgta ggcttccggg gcgacgtgga
     1441 gaatgcaggc gccgtagctg gtgccgctca tgcgggcgtc ggagattcgc accatgtcgc
     1501 gcacgccttg ttcaaccagt ttttgcggga tggggatcat gccccattcc ggcatgcccg
     1561 ggccgcccag ggggccgacg ttgcgcagga tcagcacgtg gtcgggcgtg acgtcgaggt
     1621 cgggctggtt gatgcgcgcc ttcatgtcgg ggtagctgtc gaacaccagg gccttgccgc
     1681 gatgcttgag gaaacgcttg tcggccgcgg cgggtttgat cacgcaaccg tcgggggcga
     1741 ggttgccctt gagcacggcc agtgaaccct ccgcgtagat ggggttgtcg aggctgcgga
     1801 tgacgtcggt gttgaagacc ttggcgccct ccaggttctc gcccagtgtc ttgccggtga
     1861 cggtgagcgc gtccaggtgc agcaaaggct tgagctcgct catgagtgcg cgcacgccac
     1921 cggcgtagta gaagtcttcc atcaggtatt tgtcaccgct gggacgcaca ttggcgatta
     1981 cgggcacctg gcggctggcg cgttcgaaat cgtccaggct gatgtcgcaa ttggcgcggc
     2041 gggcctgggc gatgacatgg atgatggcgt tggtcgagca gcccatggcc atggccacgg
     2101 tgatggcgtt gcggaacgcc tggacggtct gtatgcggga gggcacgagg tcttcccaga
     2161 ccatttcgac cgcgcggcgc ccgacggccg cgcacatgcg gatgtggttg acgtcggctg
     2221 cggggatgga cgaggcgccg ggcagcgtca tgcccagcgc gtcggctatc gcggtcatgg
     2281 tgctggccgt gcccatggtc atgcagtggc cgtggctgcg ggcgatgccg ccttcgatgc
     2341 ccagccattg ttcgttgttg atgtggccgg cgcggcgctc gtcccagaat ttccaggcat
     2401 ccgaacccga gcccagcgtc ttgccctccc agttgccgcg cagcatgggg ccggccggga
     2461 tgtagatggc gggcacgccc gcgctggtgg cgcccatgag caacgccggc gtggtcttgt
     2521 cgcagccgcc catgagcacg acgccgtcta tggggtggca gcggatgagc tcttcggtgt
     2581 ccatcgccag catgttccga tacagcatgg tggtgggctt gacgtactgc tcggcaagcg
     2641 acagtgcggg cagttcgatg ggcatgccgc ccgcctggag cacgccgcgt ttgacgtctt
     2701 ccacgcgttg cttgaaatgc gcgtggcacg gctggatgtc ggaccaggtg ttgatgatgc
     2761 cgatgacggg tttgccggcc cagtcgtcgg ggccgtagcc catttgcatg aagcgtgacc
     2821 gatggccgaa gccgcgcaga tcgtcgggtg cgaaccagcg cgcgctgcgc agggtgtctg
     2881 gggttttcat ggatgttcct aggttcagct ttgcatgccc cagcgccgga ccgttttctt
     2941 ctcgatcagg cggaagaaga agttctcgac cagcagtccg atgacaatga ctgtcagcaa
     3001 gccggcgaac acagcgggga tattgagcag gttgcggttc tcgaagatgt accagcccag
     3061 accgcccttg cccgaggcaa cgccgaagac cagttcggcg gcgatcaggg tgcgccaggc
     3121 gaaagcccag gcgattttca ggccggtgag gatcgagggg aacgcggcgg ggatgagaat
     3181 cctcgccacg tagctcaagc ccgtgagctc atagttgcgg ccgaccatgc gcagtgtttg
     3241 cgacacggcc aggaacccgc cctgggtgtt gagggccacg acccatacga ccgaatgcag
     3301 cagcacaaac acaatgctgg tggtgcccag gccgaaccac agcagggcca ggggcagcag
     3361 cgcgatggcg ggcagagggt tgaacatggc cgtcatgact tccaagaagt cggtgccgat
     3421 gcgggtgctg atggcgagta tggtgatgat cgccgcgatc acgatgccga tgacatagcc
     3481 cagcagcagg atacggatgg atgtccaggc gcgatcaagc agtacgccgc tgtgaatgct
     3541 gctgaaaaac gatgcgatgg tgtctgacaa ggtggggaac aacaggttgt tgtccaccgc
     3601 gcgcccatag atctgccagg ccagggccag gatgacgatg atcagcgtct tgcgcaggaa
     3661 agtattgttc gaaagttttt gccagatcga gagctggcgc accaagacgg cctttttatc
     3721 gtccgccggg gtggcttgtc gtagggtgat ggttgcgcgg cccatcatgc gtgccccatg
     3781 ttcgtatcgt tgaagagcat ttcgtggatt tccttttcca gtgacgccgc cgagccgtct
     3841 tcgtgggaga cggattcgac gtcgctgact tcggccttga cctcgccggg atgcggcgag
     3901 agcagcagga tgcggttgcc gacacggatg gcctcggcga tggagtgcgt gacgaacatc
     3961 acggtgaagc gcgtgccttc ccacagttgc agcagctcgt cctgcatctg gcgtcgggtc
     4021 agggcgtcga gtgccgcgaa aggctcgtcc atgagcagga tgtcgggttc catgcacatg
     4081 ccgcgcgcaa tggccacgcg ctgcttcatg ccgcctgaca acgtgtgggt atagctgtcg
     4141 gcgaaatttc cgagatggac tttctcgacg taggcccggg cgcgttcctc ggcctgtttt
     4201 cgcggcatct tgcgcgccac ggtgagcgga aacatgacgt tttccaggac ggtcttccag
     4261 ggcagaagct ggtcgaactc ctggaacacc atcatgcggt cggccccggg gcgtcgcacc
     4321 ggcttgccct tgatgcggat ctcgccttcc tcggggttga ggtagccacc caccgccttg
     4381 agcagtgttg acttgccaca gcccgagggg ccgagcaaca cgaatcggtc ggcgctgttc
     4441 acgttgaaac tgacccgatc cgtggcggtg acgatgctgt ccggggtttt atagcgcaag
     4501 gtgaccgaat cgacttcaag caggtgcgac atgtggggct ccgtttcctt agctgccgtt
     4561 gagcgatgcc gagacgggca gaaagtagtc cgtccatttc tgcggcaagg tcttgaggat
     4621 acccaccttg tacatgtgct gggcgaactt catggaaccg gctggcgcca tttgataggc
     4681 catcatgccg ggctgtttca acacacccat gagttcgtcg acactggtct tgtcgccgct
     4741 gagctcgcga tagatttcga ccgactcccg tgtgtgcgac ttgatgtagt cgatggcctc
     4801 tttcgaggcg gtgagcacgg cctggatgat cttggggttg gcttccgcga acttggtggt
     4861 ggtgaacaat gtggactgcg acaggccgcc ctggatgatg tcggtcgagc gcagcacaat
     4921 gtgtgcgtgg gggatggtct tgagctccct gtacgagtaa gggggggcgc cgaacgccga
     4981 ggtgatttcg ccgttggggt tggccagcat ggcggcggca tcaggatggc ccaattgcac
     5041 cgtgttggca tccagtttcg accactggtc tttgccgaac actttttcgc aggccatttg
     5101 cagcaggatg gcctgcgtgg agacgcgcac ggtgggcacc gagatcttgt ctttcgggcc
     5161 gtagtctttc agcgaatgga ttttcgggtt gcgcgtgacc agggtcaggg gcagcgccga
     5221 gttggtggtg atgcctttca ccttgccttt tgtgcggtcc cacagcaaaa gcatgttgcc
     5281 cacgccgctg ttgacgatct ggacgttgcc cgagagcagg gcgtcggttg atgcgccgcc
     5341 gctgctgaac gcgcgccagt tgaccttcag gcccttgatg ccttccttgg ccgcggcctt
     5401 ggcgatgagc ccctgctttt ccatgacgag cgcgggcagg tagatgatgc tgggttgctt
     5461 ggtgatggtg atttcatgca tttcctgggc atgggcggtc acgccggccg tggccagtgc
     5521 ggcacacaga atggccccca ggccgatatg cgggatgaac ttcatgtcgc ctccgttggt
     5581 tgaaaaaatc gtgctttgtt gttttgggat gtattacagt gagagctata ctaatatatt
     5641 agtatatgat ttacaagaga gtaaattccc caaaacacga aaatccacaa gaccttcaac
     5701 gtgcgccgaa agagagtgcc ttgaggcgag atcccccggg gatgaaaccg gaccaactat
     5761 tgacgacccc tatggccagc acactcaaac ttgaccgata ccgacaagcg gcgccacaac
     5821 tgtacgaatt gctgcgcgac aaaatcgtgg ctctggaata cctgccgggg gccgtgttgt
     5881 cgcgagtcga catctcgatg cagttcaatg tcagtcagac gccggtgcgc gaggcgttgc
     5941 tgaggcttgc cgaggagcgc ctggtcgatg tctttcccca ggcggcgacc cgggtcagcc
     6001 tgattgacac ccatatcgcc gaggaaacgc atttcctgcg gcgcgccatc gaactggaaa
     6061 ttgttcgtga gctggcgttg catggcgcgc caagcctgat tgcgacactg agggatctgg
     6121 tggccactca ggtgcggctg cgggaccgcc aggactacga tggtttcgtg gtcgccgata
     6181 gggaattcca ccgcagtctg tacgcggcgg ttggcaagga agagctatgg cggctcgtgt
     6241 gcagccgcag cggccatatc gatcggttgc gtcgattgca cttgcccgcg ggtgacaaaa
     6301 tggcccgcat cgtggacgat cacggccgca tcctggaggc gatcaccggg cgcgatcccg
     6361 gcgccgccca ggatcgcctg cgccagcact tgtcaggcac tctggaaaac atggaccgta
     6421 tccgcgacga gcatccacag tatttcgtgc aggggtaagg taaggctctt cttaccccgg
     6481 ctcgcgcgga ttcatgtgcc agccgtctta ctgcgccttc acgacctgcc gccagtggtg
     6541 cagcaggggc tcggtgtagc cgttgggctg ctccaggccc ttgaagacca ggtccgacgc
     6601 ggccttgaac gccaccgagg cgtcgaaatc cggtgccatg ggacggtagg cggggtcgcc
     6661 cgcgttctgc tgatcgacca cggccgccat gcgcttcagg gtgtcttgca cctgttcctt
     6721 cgtggtgacg ccatgcagca gccagttggc gatgtgttgg ctggagatgc gcagggtggc
     6781 gcggtcttcc atcagcccta cgttgtggat gtcgggcact ttggagcagc cgatgccctg
     6841 gtcgatccag cgcacgacgt aacccaggat accctgggcg ttgttgtcca gttcctgttg
     6901 gatttcctct gcggaccagt cggtggacgc ggccaccggc acggtcagga tggcgtcgag
     6961 aagaggctcg gacttgcctt ccatcccgcg ttgcacggct tgcacgtcga tctgatggta
     7021 gtgcagggcg tgcagcgtgg cggccgtggg tgacggcacc caggcggtgt tgccgccggc
     7081 tttcagttgc gcggatttct gttccagcat ggcggccatc aggtcgggca tggcccacat
     7141 gcctttgccg atttgcgcct ggccgcgcag gccgcattgc aggccgactt gtacgttgtt
     7201 gcgttcgtag gcgtcgatcc aggtgctgcg cttcatgtcg cccttgcgca ccatggcgcc
     7261 cgcgtgcatg gaggtgtgca tttcgtcgcc ggtgcgatca aggaaaccgg tgttgatgaa
     7321 ggccacgcgg gcggcggtgc ggccgatgca ggctttcagg ttggcgctgg tgcggcgctc
     7381 ttcgtccatg atgcccagct tgatggtgtg ggcgggcagg ttcaacagcg cctcgacacg
     7441 cgcgaagagc tcgtcggcga aggccacttc atcggggcca tgcatcttgg gcttgacgat
     7501 atagatggag ccggtgcgcg agttgcctcg gcgtttcagg tcgtgcagcg cgatcaggct
     7561 gctgacggcg gcatccagga tgccttcgaa gacttcgtgg ccgttttcat cgagaatggc
     7621 cgggttggtc atcaggtggc cgacgttgcg cacgaacagc agggcacggc cgtgcaacgc
     7681 ctgggtcttg ccttgcaggt cggtataggt gcggtcgggg ttgagggcgc gggtgaaagt
     7741 cttgccggcc ttcgaaactt cctcggtcag gttgccctgc atcaggccca gccagttgcg
     7801 gtaggcacac actttgtcgc gtgcgtcgac ggcggcgatg gagtcttcga agtccacgat
     7861 ggtggtgatg gccgcctcca tcaggatgtc cttgacgccg gcggcgtcct gctgcccgat
     7921 cgggctggac ttgtcgatct ggagctcgaa atgcaggccg ttgtgccgga acacgatggc
     7981 ttcgggcgca tcggcctgac cgcgcaagcc cacgcacaag gccgggtcgg ccaggccggt
     8041 gacggtgccg tccagcaggg tgacccgcag ggcgttgttg gccacggcgt aggccttggc
     8101 ttgagcatgc gagccttgcg ccagcggggc gttgtcgtcc aggaacttgc gcgcgtaggc
     8161 gatgactttc gcgccgcgca ccgggttgta ggtgccggcg cgcgtggcgc cgccgtcctc
     8221 ggataaggca tcggtgccgt acagggcgtc gtagaggctg ccccagcgtg cgttggcggc
     8281 gttcagcgca taacgcgcgt tggtgatggg cacgaccaat tgggggccgg cctggtgcgt
     8341 gatttcggtg tcgacctggg tcgtcttgac ggcgacgtga tcgggcacgg gagccaggta
     8401 gccgatcttt tcaaggaatg cgcggtacgc gcccgcgtcg gcgacgcgac cgggatgttg
     8461 cttgtaccat ttgtcgagtt cggcctgcag gcggtcacgc tcggccagca gtgcccgatt
     8521 gcgcggtgtc agatcatgca ccagcgcgtc cagtccggac cagaatgcgc cggcgtccag
     8581 acccgtgccc ggcagggctt cgtgctcgat gaattgatgc agcacgtcgg cgacttgcag
     8641 ccggtggtgc gcgattcgct tgtccatgat gttcctttcc gcgatggtga tgatgaagtt
     8701 gtctggcgca ggctcaaggg ccccatccgg gcagggtgtg cctggatgat gaatctcttt
     8761 ggtcggagtg atgtggccgg gtctggtcgc ccgttttcag ccgacggggc cggcggacgg
     8821 gtggtttcgt gacggcccgc cgcgcctcac ccagggctgt gcccgggcaa cgactcccgt
     8881 gcgattatag aactcttata taggacttgc ctgatgaggc ttgggtgacg ctcatataaa
     8941 gcttatgtgc ggtttgatat gtggagatgg tgcgttatgc ggattcggct ggacctgcgt
     9001 gccatgtgat gatgtggcgg gcgcgcggtc cacgaaggcc gctcccggtg tcatgaacgg
     9061 cgctggccgc cggaaatgac accgccccgg cgccatgaac ggctgccggg gcgtcgatgc
     9121 gccagcaagc ggcgaacgtg atcgtcagag gggctcgtcc tggatggtgg cgatgtgcat
     9181 caggttggtg ctgccgctgc ggccaaacgg caggccggcg atgacgatga tctggtcgcc
     9241 gttgtgcgcc agcccggtgg ccacggcttc cgaggtggcg tggtcgatca tgtcggtgac
     9301 gtggtcgagt tgggtctcga agggcagtgg gtggatgccc caggccatgg tcaggcggcg
     9361 cgcggtggcc agctccggcg tcagcgccag gatgggcgtg gtgggccgtt cgcgactggc
     9421 gcgcagggcg ctgaagcccg aggccgtgta ggccacggtg gcggccgggt cgagcaggct
     9481 ggccacgcgg cgcaacgcgc agcagatggc atctggcgtg ttgggctggg attcgccatg
     9541 ggtggcgtcc agcccggcgc gccaggccgg gtcgttctcg acctcgtgga tgatgcgctc
     9601 catgatggtg acggcctcca ccggatactc gccagaggcc gattcggccg acagcatgac
     9661 cgcatcggcg ccactgtaga tggcggtggc cacatccgag gcctcggcgc gggtggggat
     9721 gggcgcgctg atcatggatt ccagcatctg agtggccacc accacggggc ggccggccgc
     9781 ccgcgatttg cgcacgatct gatgctgcag catggggact ttctgtggcg gcagctccac
     9841 gcccaggtcg ccgcgcgcca ccatgacgcc gtcggccagt tcgatgatgg catccaggtg
     9901 gtcgatggcg gccggctttt cgagcttgac catgatccag gcgcggttgc cgatgatggc
     9961 gcgcgcctcg gtgacgtcct cgggacgctg aacgaacgac agggccaccc agtccacgcc
    10021 cagcgacagc ccatagtcga gatcgatgcg atccttcgcg gtgagcgggg agatcggcag
    10081 caccgtgccc ggcacgttga cgcccttgtg attcgacagg gtgccaccga ccatcaccgt
    10141 ggtgtcggcg aaatccggcc cgaagcggtc cacccgcaag cgcagcttgc cgtcgttgat
    10201 cagcaggtcg gcgccgactt ccagcgcggc gaagatttcg gggtgcggca gcgtggcgcg
    10261 ccgggcgtcg ccatcggtgg ggtccagatc cagccggtag ggcttgccga cttcaagttc
    10321 cacgctgccg cccttgaagt tgccgacacg cagtttgggg ccttgcaggt ccatcagcac
    10381 gccgatgggg cgccccgccg cgtgttcggc ctcgcggatg atgcgcaggc gctcggcgtg
    10441 gtcggcatgc gtgccgtggc tgaaattcag gcggaacacg tcgacgcctg cttcgatgag
    10501 cgccttgatg cgttccgggc tggagctggc cggacccagg gtggccacaa tgcgcgcgcg
    10561 tctgcttttc atggggtgcc tctttgatgt tctgtggtgt ggtgaccgtt acgcgaccag
    10621 gatggcgcgg aagtcgttga cgtttgtcag ggtgggaccc gtgaccaggg agtcgcccag
    10681 ggcttcgaag aagccgtggc cgttgttgtc gtccaggtgg tcgcgtgcct tcaggccccg
    10741 cgcgcgtgcg cgggccagcg tgtcgggcgt gatgaaggcg ccggcgattt cctccaaccc
    10801 gtccaccccg tcggtgtcgc ccgccacggc gtggatgccg tcggtgtcgt tgagcgccag
    10861 cgccagggac agcaggaact cgacgttgcg tccgccccgg ccctggccgc gcacggtgac
    10921 ggtggtctcg ccgccggaca gcagcacaca gggggggcga gcgggctgcg cgtggtggcg
    10981 ggcctgcagc gcgatgccgg ccatgacccg cccgacctcg cgggcctcac cctcgatgct
    11041 gtcgcccagg atgagggtgc gcaggcccgc ctgttcggcg acttcggccg cggcggtgag
    11101 cgccatctgg ggcgtggcga taagatgggt ttcaacacgc gccaggcgta ggtcgccggg
    11161 tttgatggtt tcgcccgcgc cggattccag caggtgccgc gcggcggcgg gcagcgtgat
    11221 ctcgtagcgg cgcacgatgt cgagcgcgtc ggcgcaagtg ctg
//
LOCUS       DASTNK010000182        48702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1654135, whole genome shotgun sequence.
ACCESSION   DASTNK010000182 DASTNK010000000
VERSION     DASTNK010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 48702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48702
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1654135"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1576
                     /locus_tag="VFR20_13155"
     CDS             <1..1576
                     /locus_tag="VFR20_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0231510.1"
                     /translation="VLRHPFLTLCSFLATLLATVALFITIPKGFFPQQDTGYIHGFAE
                     GPQDTSFGAMSKRMEALANVVRQDPDVATFGMSAGRTTFNTGTFFIGLKPKDQGRTAS
                     ADQVIDRLRPKLEKVEGARLFLQAGQDINVGGRLSRTQYQYTLTDANLDELNTWAPRL
                     LARFRQLPELTDVATDQQNQAATAVVTIDRARASTFGITPAMIDATIYDAIGQRQVAQ
                     FFTQLNSYEVVLEATPRLQKDPDLFRTLYVRSPISGDLVPLSSFVTVNTSKTGYLSIN
                     HQGQFPAVTLSFNLAPGVALGQAVDAIRHARADMNIPQTLSGSFQGTAKAFGESLATQ
                     PYLIAAALISVYIVLGLLYESYIHPLTILSTLPSAGVGALLILMLSGYDLSVIALIGI
                     ILLIGIVQKNGIMMIDFALTAERSHGLTPKDAIYQACLLRFRPIMMTTMCALLSGLPL
                     MLSHGAGAELRRPLGFAMVGGLILSQALTLFTTPVIYIYLDRTHYWYMRGKHARQVRK
                     KRRLRNARRGTAPTPR"
     gene            complement(1599..2354)
                     /gene="rph"
                     /locus_tag="VFR20_13160"
     CDS             complement(1599..2354)
                     /gene="rph"
                     /locus_tag="VFR20_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEU0231511.1"
                     /translation="MSDFPNSSESSATRPSGRQANQLRAVSIQTGYTRHAEGSVLIRM
                     GDTHVLCNASVLDKVPPFLKGAGRGWVTAEYGMLPRSTHTRSDREAARGKQSGRTQEI
                     QRLIGRSLRAVFDLVALGERTLQLDCDVLQADGGTRCASITGAWVAAALAMNTLLERG
                     ALKSNPLVDHVAAVSVGMVGGGAVLDLDYVEDSGCDTDMNVVMTGGGQFVELQGTAEG
                     RAFDRVALNQLLDLAATGIADLIGFQKQALGRA"
     gene            2552..3499
                     /locus_tag="VFR20_13165"
     CDS             2552..3499
                     /locus_tag="VFR20_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HEU0231512.1"
                     /translation="MIRSMTAYGGARVETEHGTLNIEFRTVNNRYLDVALRLPEDLRF
                     AEGMIREQLGKIVIRGKVDVRVNYARARSADDVTLDSDFLRTMADQLRVAREVIPDLR
                     APRLADVLAGPGASDGQLDPDAWLPMCQQACSRALVELQAAREREGQRLADMMLACAA
                     DVAQIVDQVETDLPTIQNDYQARITTRLREALQAANPEGFAIISGPELSARISQESVL
                     FALRIDVAEELSRLRSHISELQHILRDGTESADAGASKGGAATRKGSAGKRLDFLFQE
                     MNREANTLGSKAGALSVTHGAIDLKLLIEQMREQAQNLE"
     gene            3514..3963
                     /locus_tag="VFR20_13170"
     CDS             3514..3963
                     /locus_tag="VFR20_13170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEU0231513.1"
                     /translation="MLIRAYRPVDETTVVALWEACGLVRPWNDPHRDIERKLTEQPEL
                     FLVGELEHAVVATAMIGFDGHRGWLYYLAVAPAHQGKAFGTRLVREAETLLRQRGCPK
                     LNLLVRASNAGVIEFYRKLGYSQDEAISLGRRLIADQPSADGLGQTS"
     gene            4105..5181
                     /gene="pbpG"
                     /locus_tag="VFR20_13175"
     CDS             4105..5181
                     /gene="pbpG"
                     /locus_tag="VFR20_13175"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HEU0231514.1"
                     /translation="MILFSFLACMLGLGTAANAQTTSSQETAGTHTAKKTSSHTRHTT
                     HHRTRKAIRHKTAAHGRVIHRKRLHTVARAGDSHQVSYNVSHESKLAQRIDSSSAINS
                     LTRHTPLRSEAAFVQDLGSSAVLYSKNSDQIHPIASISKLMTAIIVLDAHQDMNQILE
                     ITEADVDNLRHSRSRLRVGTRLSRHELLHLALMSSENRAAHALARNYPGGMPAFVRAM
                     NDKARTLGMRRTHFIEPTGLSSDNVSTPRDLVKLLVAASKKPLIHRFTTDTTQQVTIA
                     KGRTLVYNNTNALMRNPGWHKDIEISKTGFINEAGECLVMLAHIDKRPIAIVLLDSTG
                     SYSRFGDAIRVRQLVEKEFNVAML"
     gene            5277..6056
                     /locus_tag="VFR20_13180"
     CDS             5277..6056
                     /locus_tag="VFR20_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HEU0231515.1"
                     /translation="MCIAYLALGRHPEWPLFIAANRDEFHARASRPAAPWSDFPNVIA
                     GLDRVAGGTWLGVTRQGRYALLTNYRDPSAFLPNAPSRGQLVGDFLIGHATPLDYAAA
                     VSDCAGIYNGFNLIVGDMNSCFYIGNRAPDASPRSLGPGSHVLSNHLLDTPWPKARRL
                     RQAMDDLALAQLNESIEPVFALLRDGAQADDADLPDTGIPRERERLLSSPFIVSPEYG
                     TRCSTVVAIHASGRAVLSEASYAPDGGMTQRHDWPFEITHA"
     gene            complement(6121..6858)
                     /gene="ypfH"
                     /locus_tag="VFR20_13185"
     CDS             complement(6121..6858)
                     /gene="ypfH"
                     /locus_tag="VFR20_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEU0231516.1"
                     /translation="MSDQSSLPPKPLVVLPEAPAPAQLFVLFHGESSSPRQLSGLAAA
                     LRQAFPQAAIVLPHGPIRIGTDIYDWVGNTSPDTRTEASRLADAVSALAGLVQQLQQH
                     YGLSGEHTALAGFSQGASLILEACTLWPDLAGRALFFSGRYDHLPLAAPPKTTLHFLH
                     GTDDDMAPVARVGEVHARLAELRGDATLDVASSVGHELHTALIAQAVLRLQTCVPLRS
                     WEKALNELEARDAGDSAVSGNEGRTLH"
     gene            7083..7811
                     /gene="imuA"
                     /locus_tag="VFR20_13190"
     CDS             7083..7811
                     /gene="imuA"
                     /locus_tag="VFR20_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HEU0231517.1"
                     /translation="MKTGAIHPTAHPPAHPERIHPALWRAASLHAPRQDTVPTGFARL
                     DHELPGLGWPLGSLVELVCDQPGIGEINLLRPALTRLAQRRIALVRPPGEPCTHCCTN
                     WRLDWRRLLWITPRSANDALWAAEQILRHDSQAILLCWVDRTPSSALRRLHLAARGGT
                     ALFFLLRPETALRQPSVAPLRLRLRPAMQGLEISIIKRQGPLCDHPVSIPLHPHAHDA
                     DHAPLDLPALAGTQPGRVHPGLAG"
     gene            7741..9213
                     /locus_tag="VFR20_13195"
     CDS             7741..9213
                     /locus_tag="VFR20_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEU0231518.1"
                     /translation="MPLWICLHLPEHSLDASIPGWRGNAPLAATLCKDRVHTCSAQAR
                     RLGVRPGLRLGTTLALAPGIVLADDDPGARDAFLRRIALGLQQYTPNIAIRGEHALVL
                     DVGASLALFHGPRALWRAFLCSANLLAPGFSAGMAPTSEGALLLALREPPGRRRALCL
                     ETLLRLLDALPVGVLPAARPYLDWLEGIGCITLAQLRKLPRKGLRQRAGPELPDSLDA
                     AYGRLQTHHAWLETPDLFSLRIDTDRLEHLHAIQAAATQLVDQLCGWLRARNAAAQAL
                     EFRFFHEKGRNAAPPTVLAIELAHPRWHSGEFKDLLIEHLRRMPPMPSIIGLALTIPR
                     LGTRPSPEAGLFPELNPAHTAEQQLLDLLCARLGAGQVRRAHPLAHHLPEKANAWVPV
                     TTQNTEAPHPASVFASDARPFWLLTAIPLNTHNDRPVYKGAALRLLRGPERLASDWWS
                     DSGHEQRDYFVAQDAHAVRYWVYRRHDPSGGRWFLHGLYG"
     gene            9232..12453
                     /locus_tag="VFR20_13200"
     CDS             9232..12453
                     /locus_tag="VFR20_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEU0231519.1"
                     /translation="MSPLPIPPRYAELDCLSNFSFLRGASHPEELIARAAKLGYEALA
                     LADDCSLAGVVRAWGEARAHGLKLIMGSRFQLAGDTLGAGDSTTHETDSPQLIVLARN
                     LNGYGNLSEFITLARSASPKGAYAAGLDLLAHPPGNQAHLRGLPDCLVIFRPRHGTGP
                     KALHQQLESLLPLFKGRLWLGLALHNRHDDAQHRATLELASRLYPVPLAAIGGVEMHA
                     RSRQPLHDTLAAIRLKTPVRDCGYALRPNAEHHLRHRMALAALYPAEALRETLVISTM
                     CSFTLDELKYRYPEEAVPAGHTAASWLRQETYLGAAKRYPQGLRGALREQLDAELNLI
                     TELGYEAYFLTIHDIVRFARQRGILCQGRGSAANSAVCYCLGITEVDPENGNLLFARF
                     ISRERNEPPDIDVDFEHDRREEVIQYVYRKYGRRRAAITAVVSTYRTRGALRDSGRAL
                     GLDPGLIDSVTASLRYRGGSQALLDELAAHPGLGGPTRTVRLWAALANGLKGFPRHLS
                     QHPGGFVICADNLSRLVPVENAAMPERSVVQWDKDDLDAMGILKVDILALGMLSALRR
                     ALELISIRRQTPFTLGDIPPRDEDTYQMIRRADTIGVFQIESRAQMSMLPRLAPRSFY
                     DLVIQVSIVRPGPIQGGMVHPYLQRRQQKAQNRPIPYANPAIERILERTLGVPIFQEQ
                     VMQIAVTAADFTPGEADQLRRAMASWRRKGGVDKFHQKLLEGMKKNGYDDAFAQSIVR
                     QLEGFGEYGFPESHAAGFAKLAYCSAWIKRHEPEAFLVALLNSQPMGFYSVNQLIQDA
                     RRHRVIILPIDINESGWDTALTPPAGPGRATMSTPNDEPQPVVRLGLHLVKGLSREAG
                     QCIEAARVQSDFSNPQDLAQRARLRRHDMDCLANANALASIAGHRRQARWQAALPRSL
                     DLLEASPIRETEQPELAAPGQGMETIADYEATQFTLGPHPLALLRPALRASQYNQARL
                     LLDGYPDRRRARACGLVTMRQRPQTAKGVIFVSLEDETGTVNVIVRPELAARQRTELL
                     CARLLGVDGVWQTHQGVGHLIAESLTNLNTMLGGLGVRSRDFH"
     gene            complement(12637..12713)
                     /locus_tag="VFR20_13205"
     tRNA            complement(12637..12713)
                     /locus_tag="VFR20_13205"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12677..12679),aa:Pro,seq:ggg)
     gene            12993..14069
                     /locus_tag="VFR20_13210"
     CDS             12993..14069
                     /locus_tag="VFR20_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEU0231520.1"
                     /translation="MTSLFDPVRLGAVSLKNRVVMAPMTRTRAAPGRVPGGMMREYYE
                     QRAGAGLILTEATSVSPQGVGYPDTPGIWSHKQVQGWQQITRSVHDKGGRIFLQLWHV
                     GRVSDPEYLNGQMPVGPSAIRCEGYVSLIQPKREYAVPRALHPAEIRAVIADFVEGAR
                     NAKEAGFDGVEIHAANGYLIDQFLRDGANHRVDAYGGGIGNRSRFLLEIVDGCIGVWG
                     SERVGVHLSPHHALHSARDSNPFALFAHVAHTLGERRIAFLVVREANDERGLTATIKQ
                     EFGGALIVNGGYDLALAQEAVGSGAADAVAFGKAFIANPDLVVRLREGAPLNVCDTST
                     FYGSGRKGYVDYPSLDGESACRLA"
     gene            14239..14640
                     /locus_tag="VFR20_13215"
     CDS             14239..14640
                     /locus_tag="VFR20_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231521.1"
                     /translation="MRFIRCGIVFATTLALAGCALGINKDSPSISYTVPRDYRTVFLR
                     VQNQADECLRGNGAYTIHSRVDAATQSGEVDVVAPFGGTVVARTVLEATDAKHTRLTQ
                     TVWGHKPWNAEALDAMRQSVRMDMSVCFAYR"
     gene            complement(14644..15108)
                     /locus_tag="VFR20_13220"
     CDS             complement(14644..15108)
                     /locus_tag="VFR20_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEU0231522.1"
                     /translation="MSNPLKTRLTDSVKDAMRAKDAPRLATLRFLLAAVKQKEVDERR
                     DLDDADIIAIIEKQVKQRRESIAAFEQAGRVETAESEKAELRILQEFLPQQASEEEVR
                     AAVDAAVAQVAAQGIGGAPAMGKVMGLVKASLAGRVDMTRVSALVKQALTGN"
     gene            15871..16341
                     /locus_tag="VFR20_13225"
     CDS             15871..16341
                     /locus_tag="VFR20_13225"
                     /inference="COORDINATES: protein motif:HMM:NF022094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HEU0231523.1"
                     /translation="MLRPRIQLGVLVLALTVLQPALAKTRAPAKPAGPTVQGTNLDAG
                     SINNEPLGGLIINRTMTVLGFDFYRSFSEVWQAVHPDSKFNITVYERPTAQYGSEIWV
                     SYRDIRVYHTFLSPARSGVRKESKLAVETAYTNITEIDAERKQFKSADLGPEEM"
     gene            16342..16698
                     /locus_tag="VFR20_13230"
     CDS             16342..16698
                     /locus_tag="VFR20_13230"
                     /inference="COORDINATES: protein motif:HMM:NF022082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgF"
                     /protein_id="HEU0231524.1"
                     /translation="MKAFNTTHIFRRVGVLAPMAAALALAAAPSMASQLIYTPVNPSF
                     GGNPLNGTYLLSKAQAENNHQAPLPDVGGLLGNMGLNPDLANTGDLQTATGTTITVQN
                     PPIGQPVTTQIGSSQK"
     gene            16708..17667
                     /locus_tag="VFR20_13235"
     CDS             16708..17667
                     /locus_tag="VFR20_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEU0231525.1"
                     /translation="MNERNFPRHRSTFRTTRLCLGLVATLALSACAIPHPPSNVSAEA
                     KLTPATPSALDLMRLPPPKKKIVAAVYGFRDETGQYKPSPDSAFSTAVTQGAASMLMK
                     ALRDSGWYIPVEREGLQSLLTERRVLRAVDERDAKSGQPAAAIPNLLPASIIFDGGII
                     AYDSNVRTGGLGAEFLGIGMSTQYSVDQVTVNLRAVDVDTGEILNSVSTTKTIYSYEI
                     HPSLFKFVNFYDLLEVEGGITHNEPAQLCVKEAIDAAVMHLTIQGLKDHEWQLKNPSD
                     WNSPIIQRYLRSASDYAVTVTPRKAISLDGEPAPAVETKKPTL"
     gene            17700..18194
                     /locus_tag="VFR20_13240"
     CDS             17700..18194
                     /locus_tag="VFR20_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231526.1"
                     /translation="MTQFRTSLTRETLSRFGRRGASLCALVLLTASPLTGYCSPDLAS
                     TSELPPSEYSNASLKASSVQFGYYNTSHISQTGARQTAEVNQSGVANTASITQTGLND
                     QAIANQFGNGNSTFAYQSASGVGVMVNQFGNQNEAMVVQTQPSPVPLVVNQIGNGYRL
                     NVIR"
     gene            18271..18753
                     /locus_tag="VFR20_13245"
     CDS             18271..18753
                     /locus_tag="VFR20_13245"
                     /inference="COORDINATES: protein motif:HMM:NF018688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231527.1"
                     /translation="MKTTLCAIASGLALAFAGTAAFATSTGSTYQGGYNNDAEISQAY
                     NGNATATIGQLGALNVAKASQDYTGNGNDITVHQFGVNNYSNSTQSHTTNAKSTTDQY
                     GFDNVANVHQSYGTNQDATAFQVGCNNTANISQMGSSLNATAMQWGVRNISTITQRGY
                     "
     gene            18848..19159
                     /gene="csgH"
                     /locus_tag="VFR20_13250"
     CDS             18848..19159
                     /gene="csgH"
                     /locus_tag="VFR20_13250"
                     /inference="COORDINATES: protein motif:HMM:NF022078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli-like amyloid fiber formation chaperone
                     CsgH"
                     /protein_id="HEU0231528.1"
                     /translation="MMAADINLQVWLETVPNTHPSVIIPYVRSKESGNLHYTLRAVKQ
                     GLGGSSRIAQSGGIQVLANQPVALSRFSLSVGKNDQCRIELILVENGNPAGTYHFDCP
                     R"
     gene            19614..20498
                     /locus_tag="VFR20_13255"
     CDS             19614..20498
                     /locus_tag="VFR20_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0231529.1"
                     /translation="MATVIVVEPNALLRLGILQLVQKMTFDITGEGIDYAQLFNGMPN
                     QQGMDLMLLSVPDAYDRMVELVNAAQQGYSPRRILLLSDTPILPYSLLNLPPVLGGYI
                     SKYSSPDVLTAAITLVLAGGKCFPSPDLAIHPEQRESESHPPAGAAPRRRWYDKGLPP
                     PKLLSGETQPDNLLTPAEPRQMRGSDHPPTVPFEAGEPQPLSPSLIASEAEMLSLTPR
                     QYEVLALLARGYPIKSVSRELNISVATTKAHTETLYQRLSVHNRNAAVYAAFSRGATL
                     GWRKADPVNGKQAKQPRS"
     gene            complement(20554..21111)
                     /gene="sufT"
                     /locus_tag="VFR20_13260"
     CDS             complement(20554..21111)
                     /gene="sufT"
                     /locus_tag="VFR20_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HEU0231530.1"
                     /translation="MRYERQEVLVSRDCPAVTVPYGSPVTIEKGCFAQITQQLGGSYT
                     VLVEGNLYRVEGVDADALGLEPTDPDKPAVPDGPVTAESVEEAAWALLATCYDPEIPI
                     DIVNLGLVYTCKVLAAGNDLYRVDVEMTLTAPGCGMGTMIADEARGKLQTIPGVDEVK
                     VDLVWDPPWSRDMISEAARLEMGLM"
     gene            complement(21124..21579)
                     /locus_tag="VFR20_13265"
     CDS             complement(21124..21579)
                     /locus_tag="VFR20_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU0231531.1"
                     /translation="MSEHFGDLRELYQELIFDHNRNPRNFHELADANRSAEGYNPLCG
                     DQLTVYLKVEDGVVSDASFVGHGCAISTASASLMTEAVKGKPVDDVEALFRDIHAMLT
                     DAHPEGRDFGKLEVLSGVREFPARVKCATLAWHTLHNAIASAGDVAQTE"
     gene            complement(21576..22835)
                     /locus_tag="VFR20_13270"
     CDS             complement(21576..22835)
                     /locus_tag="VFR20_13270"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU0231532.1"
                     /translation="MNAPDLHVRRAPGADCAADFPILTQTVRGQRLVYLDNGATTQKP
                     ASVIESEERFYRQSNANIHRGVHWLSQHATDLYEAGRERVRTLLNAARAEEIVFTRGT
                     TEAINLVAQSWGRSQLKPGDEIIVSAMEHHSNIVPWQLVCQQTGAVLKVIPINDRGEL
                     LMDEYRALLGDRTRLVAVTHISNALGTINPVAEITRLARAAGARVLIDGAQAVAHQAV
                     DVQALDCDFYAFSGHKLYGPTGIGALYARFDVLDAMPPWQGGGDMIRTVSFERTTYAA
                     VPQRFEAGTPNIAGVVGLAAAIEYVTSLGLDAIARYEHELLEQAAHAVSAIPGLRLIG
                     TAAQKAGILSFVIDGIHPHDLGTILDTHGVAIRAGHHCAMPVMTRFGIPGTARASLAV
                     YNTEQDVAALVDALGQAQKLFGVGRQA"
     gene            complement(22832..24139)
                     /gene="sufD"
                     /locus_tag="VFR20_13275"
     CDS             complement(22832..24139)
                     /gene="sufD"
                     /locus_tag="VFR20_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU0231533.1"
                     /translation="MSAAEPWSADFQARADGLFGAAVPWLAGTRRRAMDRFVAEGWPT
                     TKQEKWHHTTLAALGQTSFTAAVGTCTRSRIEQLRHGDAGHWLVFVDGRHAPELSDIG
                     TLPEGVELGPLSREAAERPERLESCLGTESDGSSVAALNLALASDGAFVRIGRGCELD
                     APVHLVFVAAAENGAGFVRNVILAEPGAHATVVEHYLGEGAEASLTSTVTRTVVAADA
                     HLTHLKLQQESPQAFHLGVLDVEQAGGSVFRSHSLSFGARLARHDIATGFSGEHCETL
                     LNGLYYVNGRRHVDHHTTIVHGQPHGTSREYYRGILADTARGVFTGRILVSPGADGTD
                     AVQRSDSLLLSRMARADARPELEIYADDVKCAHGATVGQIDENSLFYLRSRGLDETHA
                     RNLVVYAFAAEALSRIDDETLRRRASTAIQVLLPGGRELGELS"
     gene            complement(24136..24924)
                     /gene="sufC"
                     /locus_tag="VFR20_13280"
     CDS             complement(24136..24924)
                     /gene="sufC"
                     /locus_tag="VFR20_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU0231534.1"
                     /translation="MLDIKNLHASIGDKAILKGLDLSIKAGEVHAIMGPNGSGKSTLA
                     QVLAGRENFHVDQGEASWLGQDLFSLSVEERARSGLFLAFQYPIEIPGVSNAYFLKAA
                     VNAVRKHQGLSEFDAMDFIKRVKEEMKAVGMREEFLYRSVNEGFSGGEKKRNEILQMA
                     LLQPRLAILDETDSGLDIDALKAVAEGVNRQRSPDRALLVITHYQRLLDYIVPDYVHV
                     QSDGRIVRSGGRELALELEERGYGWIEPQATEAPKGKGRPGAKA"
     gene            complement(24946..26397)
                     /gene="sufB"
                     /locus_tag="VFR20_13285"
     CDS             complement(24946..26397)
                     /gene="sufB"
                     /locus_tag="VFR20_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU0231535.1"
                     /translation="MSTATEQIDSYLGSEYKPGFVTEIESDTLPKGLSEDIIRQLSAK
                     KNEPEFMLEWRLAAYRHWLTMPQPHWAKVEFPPVDFQDIRYYSAPKSKNKDGPKSLAD
                     VDPELLRTYEKLGVPLHERAKLAGVAVDAVFDSVSVATTFRKQLAEAGVIFCSFSEAV
                     KEHPDIVRKYLGSVVPPGDNFYAALNSAVFSDGSFVFIPKGVRCPMELSTYFRINAQD
                     TGQFERTLIIAEEGASVSYLEGCTAPMRDENQLHAAVVELIALDDAKIKYSTVQNWYP
                     GDKNGKGGIYNFVTKRGECRGPRSRISWTQVETGSAITWKYPSVVLRGDESVGEFYSV
                     AVTNNFQQADTGSKMTHMGRNTRSTIISKGISAGRSSNVFRGLVRVTPKAEGARNYTQ
                     CDSLLIGKKCAAHTFPTIEAQNPSAQVEHEATTSRIGEDQLFYAMQRGLSNEDAVSMI
                     VNGFCKEVFKELPMEFAVEAQNLLNVSLEGSVG"
     gene            complement(26464..26955)
                     /locus_tag="VFR20_13290"
     CDS             complement(26464..26955)
                     /locus_tag="VFR20_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HEU0231536.1"
                     /translation="MLRVSKIIDYGTLVLTYMASYPDRLFSAADLACTLGLGQPVVSK
                     VLKALGHHDLVKSSRGAKGGYALSRAPEQISIAQIIDALEEQPFGLTECSATPGACYF
                     EAGCHIRPNWQRINAIVRRTLEDTSLADMMRPTTGDRDTSGVQPQGGREVPSSAISWS
                     IRK"
     gene            complement(27104..29047)
                     /locus_tag="VFR20_13295"
     CDS             complement(27104..29047)
                     /locus_tag="VFR20_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEU0231537.1"
                     /translation="MPFFRFSRTPRLRNDFRTLASLLPYIWEYKARVGVAVACLVLAK
                     VANVVLPIFLKDVVDRLSVPATLLALPVAALLGYGLARLATSVFAELRDALFARVTQG
                     SIRRIAARVFEHLLLLSMRFHMQRQTGGLSRDIERGTKGIGFLLNFMVFNILPTLLEI
                     TMVTAILLWRYNYLFAVVTLGTIACYIVFTLLVTEKRMVYRRSMNTLDSSANNKAVDA
                     LINYETVKYFGNEGYELGRYDRNLAKWVDSAVKNQVSLNALNMGQGIIITIGITLLLW
                     LSARGVVHRTMTVGDVVLVSAYLTQLYAPLNFLGFVYREIKNALADMERMFGLLEQDQ
                     EVADRPGAQALDTRQAEVRFDCVSFGYEANRQILFDVDLAIPAGHTLAVVGASGAGKS
                     TLSRLLFRFYDATAGAIRINGTDVRDLTQISLRHHIGIVPQDTVLFNDTILYNISYGR
                     TGATFEDVERVARAASIHDFIMSLPEGYQTQVGERGLKLSGGEKQRVAIARTLLKDPP
                     ILILDEATSALDTRTERAIQRELGQIARDRTTLIIAHRLSTIVDADEIVVLDQGRVLE
                     RGTHWALLRTQGRYAQMWRMQQADEREQGVPIGREGGGGVSDGTPGRREAGRESARPD
                     AMDVDEWGDGAGYGSGGRPADAT"
     gene            29237..29752
                     /locus_tag="VFR20_13300"
     CDS             29237..29752
                     /locus_tag="VFR20_13300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0231538.1"
                     /translation="MAVSPFDYEATLLACAAGKQNALRDLFQHEAPRMLALSTKMFGR
                     REEAEDAVRDTFILLWKNAGNYDESTGTARAWIYSILRYRVLSQIRQQSHNPGDDTTS
                     ISSQTDVKACLSTPVKSLAQLDDMQRAPLLMAFYHGQTFNQMAARLMTPVELIRTQLR
                     TGLDAVQGRHA"
     gene            29749..30639
                     /locus_tag="VFR20_13305"
     CDS             29749..30639
                     /locus_tag="VFR20_13305"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEU0231539.1"
                     /translation="MSIERGSEHDFLIAEYTLGLLDSHESAQVNQLLASDSQAASTAL
                     KWEGLFLSLTDELEPINPPPHLLQRIQASLGHEISLVKRRRASPGILRKIGSATLGGL
                     WFWRLLSVVLAITAATYALNQNPTVVVAADKPKPNVTVVNTPAPTHVAILQAPGQTST
                     PGWIVTLDEKHDLVMTPEVHIDVPTDSAVQLWTHNPRDRTPRSLGIVPANQATVVPAA
                     TLGDIADDQFFEMTQEQAGGSPSGAPTGPILFLGRMVELRPKPEKSNGIQAVGAPASA
                     PAAGGQPIDTNTSDNQPNGK"
     gene            complement(30613..31386)
                     /locus_tag="VFR20_13310"
     CDS             complement(30613..31386)
                     /locus_tag="VFR20_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU0231540.1"
                     /translation="MEAYRIISGFAVGLLVGVTGVGGGSLMTPLLTLGFGYTPIVAVG
                     TDLAFAAVTKSVGTLAHGRYGQVHWRAVASLCAGSLPAALATLFFIRYAGPASLGLMH
                     AIRVIIGVSVLLTVLALALRTRLLARLSRCSAYPLRGRAQVLATMAAGAVLGVLITLS
                     SIGAGAIGATLIFLLYPKLAPAEVAGTDIAYAVPLTVVAAAGHWWLGHLDWSLLGTLL
                     LGSVPGIWLGARLARALPQRYVRATLATTLTLLAVRLIV"
     gene            complement(31466..32851)
                     /locus_tag="VFR20_13315"
     CDS             complement(31466..32851)
                     /locus_tag="VFR20_13315"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEU0231541.1"
                     /translation="MTLSVFDIFKVGIGPSSSHTVGPMRAARNFVRNLKSRGELSRVA
                     RLTIDLYGSLAETGRGHGTDIGILLGLSGEAPDTVQPADIQSIVARVEASGSLMLWHE
                     HAIAFDLGKALSYRIRPMVEHPNGMRFSAFDESRQPIIVERYVSIGGGFIRALDAIDT
                     DETVPRHAPMPYPFTTGAELLAMCAETGLSVAALAMANEMALRSEDEVRAGVWHIADV
                     MNQCIDRGCGIDNPHADDPLPGPLGVKRRAPTLYRELQRDKPGNDLMYAMDWVNLFAM
                     AVNEENASMGRVVTAPTNGAAGLLPAVLRYYQDFILGSSKDGMVEFLLAAAAIGFLYK
                     RNASISGAEVGCQGEIGSACSMAAAGLTAALGGTPRQVENAAEIGMEHNLGLTCDPIG
                     GLVQIPCIERNAIGAVKAINASRLALRGDGTHKVSLDAVIETMRQTGADMRSKYKETS
                     RGGLAVNVVVC"
     gene            complement(32832..33617)
                     /locus_tag="VFR20_13320"
     CDS             complement(32832..33617)
                     /locus_tag="VFR20_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEU0231542.1"
                     /translation="MRTQALAALLLADPDQKLAMVRSLAVSSPVDAGARLDEPPGVPG
                     RPIRPERVPPFKVRQRSVHTLEGRAALVHSLAHIEFNAINLALDAVWRFPGMPDAYYA
                     DWLRVARDEACHFGLLRRHLRTLGHDYGDFPAHDGLWDMAWRTRDDIMARLALVPRTL
                     EARGLDASPIVRDKLEAVGDAAGANLIEIILHDEIGHVAVGNFWYRYLCDKAGLDPLS
                     TYRSLAVIHRAPRLRGPFNTQARRAAGFTEQELEALRHDALGV"
     gene            complement(33654..34049)
                     /locus_tag="VFR20_13325"
     CDS             complement(33654..34049)
                     /locus_tag="VFR20_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU0231543.1"
                     /translation="MSSSETPVVLPPIPAKRYFTIGEVSELCCVKPHVLRYWEQEFTQ
                     LKPVKRRGNRRYYQHHEVLLIRRIRDLLYDQGFTISGARNRLGDSREAPHDQDAAVRL
                     SAVELQALRTDLQTVRDMLSESLGDSSLT"
     gene            complement(34046..34378)
                     /locus_tag="VFR20_13330"
     CDS             complement(34046..34378)
                     /locus_tag="VFR20_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEU0231544.1"
                     /translation="MTTDPRTLTKAELAELLFDRVGLNKREAKDIVDTFFEEIRDSLV
                     RGVEVKLSGFGNFQVRDKPPRPGRNPKTGEIIPIAARRVVTFHASQKLKSTVERTSVA
                     GETIQDRP"
     gene            complement(34413..36827)
                     /gene="pheT"
                     /locus_tag="VFR20_13335"
     CDS             complement(34413..36827)
                     /gene="pheT"
                     /locus_tag="VFR20_13335"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU0231545.1"
                     /translation="MQVPESWLRTFANPDIDSETLSHRLTMAGLEVEEAVPAAPPFNG
                     VVVGLITDIAPHPNADKLRVCQVDDGSGTSLQIVCGAPNAAAGLKVPLARVGATLPGG
                     VTIGAVKMRGVESAGMLCSARELGLSEDHAGLLPLDADLVPGVSVRDALDLDDTVFTL
                     KLTPNRADCLSILGVAREVSALTSVPLTMPDIGRASVTLNDRLPVSVQAPDLCGRFAG
                     RVVRGVNARAATPAWMRARLERAGQRSISALVDISNYVMLELGRPTHVFDLDRVKGGL
                     TVRWAREGESLVLLNGQTVVLDPQVGIIEADGTVESLAGIMGGEASSVTLDTTNVYLE
                     AAFWWPEAIMGRARRYKFSSEASHRFERGVDFDAIPRDLERMTALILQICGGQAGPVD
                     DQIVNLPERRPVEMRLARCHRILGVPVTREEVAGIFSSLGLAFEERDGVFAVTPPSYR
                     FDLAIEEDLIEEVARIHGFERIPDVPPRAPAKMRAVPEEARGPHELRGVMAGLDYQEV
                     INFSFVEESWERDYAGNANPIRLLNPIASQLAVMRSSLLGGLVANIRYNANRKQTRVR
                     VFELGRVFMRDAEVADGPLSVGGVGQPQRLAGAAWGPAREEQWGCAVRQVDFHDVKKD
                     VETLLGARAPRLCCVAAPHPSLHPGRAARLELDGRAIGWLGELHPRWAQQMDLTHPPV
                     VFELDVEGISRADLARLAPFPRQPVAVRDLAVWVDARVPFQAMSDTLAQAIASDNTLD
                     VVRDFRLFDVWRDMSVSSGAPEKSMAFRFWLQGRDTTLDDATVERCVEKLLNALIHAH
                     AARRRA"
     gene            complement(36870..37892)
                     /gene="pheS"
                     /locus_tag="VFR20_13340"
     CDS             complement(36870..37892)
                     /gene="pheS"
                     /locus_tag="VFR20_13340"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU0231546.1"
                     /translation="MIAASDDLIDQAREQFAQASDAAALENAKARFLGRQGALTALLK
                     GLARLDPEQKRAEGARINQLKQRVEALLDQRRADLAQAELDRRLAAETVDVTLPGRGR
                     GRGGIHPVIQTWQRVEAIFRSIGFDVADGPEIENDWTNFTALNNPENHPARSMQDTFY
                     VDMNDAQGLPLVLRTHTSPMQVRYARMNTPPIKVIAPGRTYRVDSDATHSPMFHQVEG
                     LWISDDISFADLKGVYTDFLRAFFETDDLSVRFRPSFFPFTEPSAEVDMMFTSGPNRG
                     RWLEISGSGQVHPQVVRNFGLDPERYIGFAFGSGLERLTMLRYGVDDLRQFFEGDLRF
                     LSQFNR"
     gene            complement(37965..38324)
                     /gene="rplT"
                     /locus_tag="VFR20_13345"
     CDS             complement(37965..38324)
                     /gene="rplT"
                     /locus_tag="VFR20_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU0231547.1"
                     /translation="MPRVKRGVTARARHKKVINAAKGYRGRRGNVFRIAKQAVMRAGQ
                     YAYRDRRNKKRTFRALWITRINAACRELDVTYSVFIAGLKKASIELDRKVLADMAVHD
                     KAGFAAVVAQAKSALAS"
     gene            complement(38344..38541)
                     /gene="rpmI"
                     /locus_tag="VFR20_13350"
     CDS             complement(38344..38541)
                     /gene="rpmI"
                     /locus_tag="VFR20_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU0231548.1"
                     /translation="MPKMKTKRGAAKRFKVRASGSIKRGQAFKRHILTKKTTKSKRQL
                     RGSAAVHDSDVASVKAMMPFA"
     gene            complement(38759..40303)
                     /locus_tag="VFR20_13355"
     CDS             complement(38759..40303)
                     /locus_tag="VFR20_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEU0231549.1"
                     /translation="MSLAVLASRALCGLEAVSVRVETHVSSGLPAFHVVGLPDAGVRE
                     SRERVRSALLSSGFEFPAGRITVNLAPADLPKESGRFDLPIALGVLLASGQIPVVAPA
                     PPGIDGAGEGNNALTLANHVFAGELSLTGAVVAVGAPLAIALAVAREHPHAQLVLPAA
                     SAGTAASVPGLSVLAASSLAQVVAHLSGGERLAMAAPRTISTDGSTAPCLSDVRGQAL
                     ARRVLEVAAAGAHSLLMAGPPGTGKSMLAHRLPGLLPRLGQDQVLEVAALAGIGGREY
                     RFTDVPPFRAPHHSASVPALVGGGIHPRPGEISLAHRGVLFLDELPEFDRRALEALRE
                     PLETGCVSIARAARSLTFPAAFQLVAAMNPCPCGWHGHPHMRCACTPDRIEKYRGRLS
                     GPLLDRIDLQISLPSADAHWMDAPAGEASMPVRIRVEACRRRQLVRQGVPNALLGVAG
                     IDAHCQPDTAAKVILRQAMSRWNWSARVVHRVLRVARTVADLDDAHLVGGTHVAEAIQ
                     YRQPWR"
     gene            complement(40434..40676)
                     /locus_tag="VFR20_13360"
     CDS             complement(40434..40676)
                     /locus_tag="VFR20_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEU0231550.1"
                     /translation="MVNRADWFEDFQKNLSDLIAKSPAADIERNIKAMMTQTFSKMDL
                     ITREEFDVQADLLNRAMARIVSLEAKVQELEARHGN"
     gene            complement(40736..41140)
                     /locus_tag="VFR20_13365"
     CDS             complement(40736..41140)
                     /locus_tag="VFR20_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231551.1"
                     /translation="MQNTHKYFAVFAIAASLSLLAACSKSDDASVTSSGAPTATQPAA
                     PESTPTPAPSTQPAPAPSSSSSSDSNTMSKANSAADTVAQKAGELKNSAAETLDSMGH
                     AIKKGAAEANQKIQGAVGNGTATPSTPSANNH"
     gene            complement(41438..43168)
                     /gene="ptsP"
                     /locus_tag="VFR20_13370"
     CDS             complement(41438..43168)
                     /gene="ptsP"
                     /locus_tag="VFR20_13370"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU0231552.1"
                     /translation="MLCLQGQGVVKGIAIGRAVVMSAAALEVAHYRIQPDDIQAESDR
                     LRAALATASRELQDMVGSLPDDAPRELGALLTVHSMLLDDPTLSEQTCELIAQRQYNA
                     EWALTTQGQILGEQFSAIEDEYLRERGGDIRQVIERVLRVLSGSSALLPTLNAVVPNL
                     TQSDGSLIVVARDISPADMLRLRGGRFGAFLTDLGGATSHTAIVARSMNVPAVVGLGN
                     VRTLVRDGDVLIADGANGMVLVNPSAAVLDEYHRRQAEFAEERAELALLRDAPAVTLD
                     GIHIGLHANIELPEEAAAALQAGAEGIGLFRSEFLFMGRADLPGEEEQYQAYASVVRV
                     MAGRPVTIRTLDLGSDKTLDGEATVATNPALGLRAIRYCLANPELFGTQLRALLRAAQ
                     HGPIRILVPMVSHMREVIATRQALEAAWHELDARGVAYSRDIALGAMVEVPAIAIAID
                     PFVECLDFLSIGTNDLIQYTLAIDRSDSDVGDLYDPMHPAVLRLIANTINVGERAGKP
                     VAVCGEMAGDASMTRMLLGLGLKEFSMHPQQLLDVKKEIRRAHSNALRVKVASALNRA
                     ERIDLAALAG"
     gene            complement(43242..43511)
                     /locus_tag="VFR20_13375"
     CDS             complement(43242..43511)
                     /locus_tag="VFR20_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEU0231553.1"
                     /translation="MPITDIVVQNKLGLHARAAAKLTQLASQFKSEIFIARGAQRVNA
                     KSIMGVMMLAAGLGVTVKIEASGQDADQALSQIEALFSNKFGEQE"
     gene            complement(43560..43961)
                     /locus_tag="VFR20_13380"
     CDS             complement(43560..43961)
                     /locus_tag="VFR20_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU0231554.1"
                     /translation="MVRIILIMHAPLGSAFFQCAEHVLGHVPGLTVVDILPTDEPARK
                     TEEIADVIASRPDEPVLILCDLFGATPFNIASQALRVATQRGGRGHLVTGTNLCMVLK
                     ALTERQENPDKLTEKVRLGALKGIIDADHKC"
     gene            complement(43964..44923)
                     /gene="gshB"
                     /locus_tag="VFR20_13385"
     CDS             complement(43964..44923)
                     /gene="gshB"
                     /locus_tag="VFR20_13385"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEU0231555.1"
                     /translation="MHVLFIIDPLPLLKAYKDSSVAMMRALAHRGHTFSVAMMGGLYI
                     DEGVVKARAAGIALRPDADLHGHGWWNETVPAADTDLGAFDAVIMRKDPPFDMEYIYA
                     THMLEYAEAQGARVFNSGAGIRNHPEKLAITEFSEFTVPTLVTSDMSRLRAFHARQGD
                     VIVKPLDGMGGTGIFRVRPEEPNLGVILETLTANGSRTIMAQRYIPEIVDGDKRILIV
                     GGEAVPYALARIPLAGETRGNLAAGGRGVARPLSDHDKEIAAVVGPRLARRGLLLVGL
                     DIIGQYVTEINVTSPTCFVEITEQTGFDVAGFFVSALERAVGI"
     gene            complement(44987..45559)
                     /gene="infC"
                     /locus_tag="VFR20_13390"
     CDS             complement(44987..45559)
                     /gene="infC"
                     /locus_tag="VFR20_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU0231556.1"
                     /translation="MLGVSDIATDKPNRINGEIHVPEVRLIGVDGEQLGIVQTSEALR
                     LSEQHEVDLVEIAPNAEPPVCRLMDYGKFKYQEQKRQQEARAKQKVIQVKEVKFRPGT
                     DEGDYQVKLRNLRRFIEEGDKAKVTLRFRGREMAHQELGMRVLQRVRDDLVELCQVEA
                     MPKLEGRQMVMVLAPRKKQPAPGKQTNSAS"
     gene            complement(45586..47508)
                     /gene="thrS"
                     /locus_tag="VFR20_13395"
     CDS             complement(45586..47508)
                     /gene="thrS"
                     /locus_tag="VFR20_13395"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU0231557.1"
                     /translation="MVHITLPDGSVREYSDPVSVGDVASSIGAGLGRAALAGKVGFDG
                     EAQKLVDTSFRIDRDASLAIITAKDADGLDLIRHSTAHLLAYAVKSLFPDAQVTIGPV
                     IENGFYYDFSFKRPFTPEDLEAIENKMAELARKDEPVTREEWGRDDAVKFFEGQGEHY
                     KAEIIASIPANQTISLYREGDFIDLCRGPHVPSTGRLKVFKLMKVAGAYWRGDSNNEM
                     LQRIYGTAWATKEEQSAYLTMLEEAEKRDHRKLGRELDLYHFQDEAPGLIFWHPKGWT
                     LWQQVEQYMRTIYRDNGYEEVKAPQILDLSLWKKTGHWDNYAENMFTTESENRVYGLK
                     PMNCPGHVQIFKAGLRSYRDLPVRYGEFGQCHRNEPSGSLHGLMRVRGFTQDDGHIFC
                     TEDQLQEECAVYTLLLQKVYADFGFTDILYKIATRPDKRIGSDEVWDKAERALMESLE
                     RTGCDYVVSPGEGAFYGPKIEYTLRDAIGRQWQCGTMQVDFSMPQRLGAEYVDANDQR
                     CTPVMLHRAILGSLERFIGMLIEHHAGAMPAWLAPEHAVVCCISEHSAEYAAEVVATL
                     KKQGFRASADLRGEKITRKIREHSMQKIPYILVVGEKEREAGTVAVRQRGGADLGAMV
                     LADFTQHLRQEIDARR"
     gene            complement(47888..47964)
                     /locus_tag="VFR20_13400"
     tRNA            complement(47888..47964)
                     /locus_tag="VFR20_13400"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47928..47930),aa:Val,seq:gac)
     gene            complement(48147..>48702)
                     /locus_tag="VFR20_13405"
     CDS             complement(48147..>48702)
                     /locus_tag="VFR20_13405"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU0231558.1"
                     /translation="RVVSVEQARALRQTVPAFVSAVALFVDPTVEEVNRVIDQVRPDL
                     LQFHGNETPEFCRRFAHRYIRAFRVGAPGLATPEGLLAQCRRYDDAAGWLFDSFSSGF
                     GGSGRAFDVSLLRDVLACEQARPVILAGGLSAATLGTALDAVHPFAVDVSSGVEDSPG
                     IKSDERVAAFIGGVREHDSLRRPT"
BASE COUNT         9512 a        15947 c        14396 g         8847 t
ORIGIN      
        1 ggtgctgcgc cacccgtttc tcacactgtg ctcgttcctg gccacgctcc tggccaccgt
       61 cgcgctgttc atcacgattc ccaaggggtt ctttccccag caggacaccg gctacatcca
      121 tggcttcgcc gaaggcccgc aagacacgtc cttcggggca atgtccaagc gcatggaggc
      181 gctcgccaac gtggtccggc aagacccgga cgtggcgaca ttcggcatga gcgccggccg
      241 caccacattc aacaccggca ccttcttcat cggcttgaag ccaaaggacc agggccgcac
      301 cgccagcgcg gaccaggtca tcgaccgcct gcggcccaag ctcgagaagg tcgagggcgc
      361 acgattgttc cttcaagcgg gccaggacat caacgtgggc gggcggctct cgcgcacgca
      421 ataccagtac accttgaccg acgccaacct ggatgagctc aacacctggg cgccgcggct
      481 gcttgcgcgt tttcggcaat tgcccgagct caccgacgtg gcgacagacc agcagaacca
      541 ggccgccacg gccgtggtga cgatcgatcg cgcgcgcgcc tcgaccttcg gcatcacgcc
      601 ggccatgatc gatgccacca tctatgacgc catcggtcaa cgccaggtgg cgcagttctt
      661 cacccagctc aacagctacg aagtagtgct ggaagccacc ccgcgactgc agaaggatcc
      721 cgatctgttc cgcacgctat atgttcgatc gccgatctcc ggagacctgg ttccgctttc
      781 gagcttcgtg acggtgaaca catcgaagac cggctatctg tcgatcaatc accagggaca
      841 gttccctgcg gtcaccctgt cgttcaatct tgcgccaggc gtcgccctgg ggcaggccgt
      901 cgacgccatc cggcacgccc gggctgacat gaacatcccg caaacactca gcgggtcatt
      961 ccagggcaca gccaaggcgt ttggcgagtc gctggccacg cagccctacc tgatcgccgc
     1021 cgccctgatc tcggtctaca tcgtgctggg gctgctgtac gaaagctaca tccatccgct
     1081 gaccattctc tcgaccctgc cgtcggcggg ggtcggagcc ctgctcattc tgatgctcag
     1141 cggctacgac ctgagcgtga tcgcactgat tggcatcatt ctgctgatcg gcatcgtgca
     1201 gaagaatggc atcatgatga tcgacttcgc cctgacggcc gagcgcagcc acggcctgac
     1261 accgaaagac gccatttatc aggcctgcct gctgcgtttc cggcccatca tgatgaccac
     1321 catgtgcgcc ctgctcagcg gcctgccgct gatgctcagc cacggcgcgg gggccgaact
     1381 gcggcgccca ctgggctttg cgatggtggg cggcctgatc ctgtcacagg cgttgaccct
     1441 gttcaccaca cctgtcatct acatctatct ggatcgcacg cattactggt acatgcgggg
     1501 caaacacgcg agacaagtca gaaagaagcg gcggttgcgc aatgcgcgac gcggcacggc
     1561 gccaacgcca cggtagcgtg cagtgtggtt gagtggggtc aggcccggcc cagcgcctgt
     1621 ttctggaacc cgatcaggtc cgcgatacct gtggcggcca gatccagcaa ctgattcaag
     1681 gcgacgcgat caaaagcgcg cccctcggcc gtgccctgca attccacgaa ttggccgcca
     1741 ccggtcatga ccacattcat gtctgtgtcg cagccggaat cctcaacgta atcgaggtcg
     1801 agcaccgccc cgccaccaac catgccaacc gacaccgccg cgacgtgatc gaccagcggg
     1861 ttggacttca gcgcgccgcg ctcgagcagc gtgttcatcg ccagcgccgc cgccacccag
     1921 gcgccggtga tgctggcgca gcgggtcccc ccgtcggcct gcagcacgtc gcaatccagt
     1981 tgcagcgtgc gttcaccaag cgccaccaga tcgaacaccg cgcgcaggct gcggccgatc
     2041 aaccgctgga tttcctgagt gcgtccgctt tgcttgccac gcgccgcctc gcggtcggag
     2101 cgcgtgtgcg tggagcgcgg caacatgccg tattccgccg tcacccagcc ccgccccgcc
     2161 cctttcagga acggcggtac tttgtccagc acactggcgt tgcacagcac atgcgtgtca
     2221 cccatgcgga tcaagaccga tccttcggca tggcgggtgt agccggtctg gatggaaacc
     2281 gcccgcaatt ggttggcctg gcggcctgag ggccgggtag cggatgattc ggaggaattc
     2341 ggaaaatcag acaagcggcg ctcctggaca acttcaaggg tgaaatagac aatcattcag
     2401 attttcgcac aggaacgcca tggcatcggt gtgcgcacgg ccactcactg cccgcgacac
     2461 ccgggttccg cccccctgca ttgacccgct tgcttttaca atacgccgtc gcgcttgcgc
     2521 cccggcgacg ccaccctcaa aggacctctc catgatccgc agcatgaccg cctacggcgg
     2581 cgcccgcgtg gaaaccgaac acggcacgct caacatcgaa ttccgcaccg tcaacaaccg
     2641 ctacctggac gtggcgctgc gattgcccga ggatttgcgt ttcgccgaag gcatgatccg
     2701 cgaacaactc gggaaaatcg tcatacgggg caaggtggac gtgcgcgtga actatgcccg
     2761 ggcacggtcg gccgacgatg tcacactgga ctccgatttt ctgcgcacca tggcggatca
     2821 actacgcgtc gcccgggaag tcattcccga cctgcgcgcg ccccgcctgg ccgacgtgct
     2881 ggcggggccc ggcgcctctg acggccagct cgacccggac gcctggttgc ccatgtgcca
     2941 gcaggcttgt tcccgggccc tggtcgaact gcaagcggcg cgggagcgcg aaggccaacg
     3001 cctggctgac atgatgctgg cctgcgccgc cgacgtcgcg caaatcgtcg atcaggtgga
     3061 aacggatctg cccaccatcc agaacgacta tcaggctcgg atcaccacac gactaaggga
     3121 agcgctgcaa gccgccaacc cggaagggtt cgccatcata agtggcccag agctatccgc
     3181 ccgcatcagc caggaaagcg tgttgttcgc cttgcgcatc gacgtggccg aagaattatc
     3241 ccggctacgt tcgcatattt ccgagcttca acacattttg cgggatggca ccgaatccgc
     3301 tgacgcgggc gcatcaaaag gcggcgccgc gacgcgcaaa ggcagcgccg gcaaaagatt
     3361 ggacttcctg ttccaggaaa tgaatcgtga agccaatacc ctgggttcca aggcgggggc
     3421 gctgagcgtc acccacggcg ccatagactt gaagctgctg atcgaacaga tgcgcgagca
     3481 ggcgcagaac ctcgaataaa aggcccacca cccatgctga tccgcgctta tcgcccggtc
     3541 gatgaaacta ccgtcgtcgc cttgtgggag gcctgcggcc tcgtacgccc ctggaacgac
     3601 ccgcatcgcg acatcgagcg caaactgacc gagcaacccg agctgttcct cgttggcgag
     3661 ttggagcacg cagtcgtcgc cacggccatg atcggcttcg atggccatcg cggttggttg
     3721 tattacctgg ccgtggcgcc cgcccatcaa ggcaaagcct tcggcacccg cctcgtgcgg
     3781 gaagccgaaa cactgctgcg gcagcgcgga tgccccaagc tgaatttgct ggtgcgcgca
     3841 tccaacgcgg gcgtcatcga gttctatcgc aagctgggct acagccagga cgaagccatc
     3901 agcctgggac ggcgcctgat agcagatcaa ccctctgccg acggtctcgg ccagacctcc
     3961 taacggaaaa ttcaagtaat gcttgtaacc gtttaaatat attggtgttt ttaaaaaatt
     4021 ctgatactat ctgccccatc tcgaaaaact ttttcaatac ccgtatcggc catcatgcgt
     4081 aatatctgga accggaccac gcgtttgatt ctgttctctt tcctggcctg catgcttggg
     4141 ctgggtacgg ccgccaacgc gcagaccacc tcttcccaag aaaccgccgg cacgcacaca
     4201 gccaagaaaa catcctcgca cacacgacac accacgcatc accgcacccg aaaagccatt
     4261 cggcacaaaa ccgccgccca tggccgcgtc atccatcgca agcgcctgca tacggtggcg
     4321 cgcgcgggcg actcacacca ggtgtcgtac aacgtcagcc acgaaagcaa gcttgcccag
     4381 cgcatcgact ccagcagcgc aatcaattcg ctgacgcgcc acacccccct ccgctccgaa
     4441 gccgcgttcg tccaggatct tggcagttcc gctgttctgt actccaagaa cagtgaccag
     4501 atccacccca tcgcttccat ttccaagctg atgacggcca tcatcgttct ggacgcgcac
     4561 caggacatga accaaatcct ggaaatcacc gaagccgacg tcgacaacct gcgtcattca
     4621 cgttcgcgcc tgcgggtcgg cacccggctc agccgacatg aattgctgca tctggcgctg
     4681 atgtcctccg aaaaccgcgc agcccatgcc ttggcgcgca attatcctgg cggcatgccg
     4741 gcgttcgtgc gcgccatgaa cgacaaggcg cgcacgctgg gcatgcgccg cacgcatttc
     4801 atcgaaccca ccggcctgtc cagcgacaac gtctccaccc cgcgcgatct ggtcaaactg
     4861 ctggtcgcgg ccagcaagaa gccactcatc caccgcttca ccaccgatac cacccaacag
     4921 gtcaccatcg ccaagggtcg caccctggtc tacaacaaca ccaatgccct gatgcgcaat
     4981 cccggctggc acaaggatat cgagatctcg aaaaccggct ttatcaacga agcaggtgaa
     5041 tgcctggtca tgctggccca catcgacaag cgccccatag ccatcgtcct gctcgactcc
     5101 accggaagct attctcgctt tggcgacgcc atacgggttc gccagttggt cgagaaggaa
     5161 ttcaacgtcg ccatgctcta gggcccgacc cctatccacc tgtcagcgca aggcttcttt
     5221 tagtgtgagc aggacactag gctggatttg acccggatct agtctagaat ccagctatgt
     5281 gcatagcgta tctggctctg ggccgccatc cggaatggcc gttgttcatc gccgccaatc
     5341 gtgacgaatt ccatgcccgg gccagccggc cagccgcgcc atggtctgat tttcccaatg
     5401 tcatcgcagg ccttgaccgc gtcgcgggcg gcacttggct gggggtgaca cgccagggcc
     5461 gctacgcgct cctgaccaac taccgcgatc catccgcctt tctgccgaac gcaccgtcgc
     5521 gcggacaact ggtgggcgat ttcctgatcg gccacgccac gcctttggac tatgccgcgg
     5581 cagtgtcgga ctgcgccggc atctataacg gtttcaacct gatcgtcggc gacatgaata
     5641 gttgtttcta tatcggcaat cgcgcccccg acgcctctcc ccgctcattg ggacccggca
     5701 gccatgtgct gtcgaaccat cttctggata ccccatggcc caaagccagg cggttgcgcc
     5761 aagccatgga tgatctcgcg ctggcacaac tgaacgaatc gatcgaacct gtgttcgcct
     5821 tgctcaggga tggtgcccaa gccgatgatg ccgatctgcc cgacaccggc atcccgcgcg
     5881 agcgcgagcg cctgctgagc agcccgttca tcgtgagccc cgaatacggc acccggtgct
     5941 cgaccgtcgt cgccatccac gcgagcgggc gcgcggtctt gagcgaggcc agttacgctc
     6001 ctgatggggg catgacgcag cggcatgact ggccgttcga aatcacccac gcctaatgca
     6061 ccgtttggtt caagtgccta cacaccttga ggctctgcaa gtgcaacgtc gttacgatca
     6121 ttaatgcaac gtacggccct cgttgcctga tacggcggaa tcaccggcat cacgggcctc
     6181 cagctcattc agtgcttttt cccagctgcg caatggcaca caggtttgta agcgcagcac
     6241 ggcctgtgca atcaacgccg tgtgtagttc atggccgacg gacgatgcca cgtccagcgt
     6301 ggcatcgccc cgcaattccg ccagcctggc atggacctca ccgacccgcg ccaccggcgc
     6361 catgtcgtcg tcggtcccgt gcaggaaatg cagggtcgtt ttcggcggcg ccgccaaagg
     6421 caggtgatca taccggcccg agaagaacaa ggcccgcccg gcaagatcgg gccatagggt
     6481 gcaggcttcc agtatgagcg atgccccctg ggagaatccc gccaacgccg tgtgttcacc
     6541 cgacagccca tagtgctgct gaagctgctg gaccaaacca gccaacgccg atacggcatc
     6601 ggccagtcgt gaggcctcgg tccgggtatc gggggaggta ttgccgaccc agtcgtagat
     6661 atcggtcccg atccggatcg ggccgtgggg caacacgatg gcggcctgcg gaaaggcctg
     6721 cctgagcgcg gcggccagcc cgctcagttg cctggggctt gaggattcgc catggaacag
     6781 cacgaaaagc tgggcgggag ccggcgcctc tggcaagaca accaagggct tgggaggtaa
     6841 tgatgattga tcagacatga aatcgagcgc aagccaccgt acatgaaaat ccgccacgcg
     6901 actgcgtgcc cgcacccggt gtcgtgcctc acaagctggt tgatggcgga ttcgacatat
     6961 tcaaaacaac cgacgtttcg atactagcat tgttgatctc ttgcggtcag gtggtttcag
     7021 gatcaaaata actgtgtttt tatacagtaa aattaccgca tcacccacaa tcctgtctgg
     7081 ccatgaaaac cggagcaatc catccaacag cgcatccacc ggcgcacccc gagcgcatac
     7141 accccgcgct ctggcgcgcg gccagcctgc acgcgcctcg ccaggatacc gttcccactg
     7201 gctttgcccg gcttgatcac gagcttcccg gcctgggttg gccactgggt tcgctcgtgg
     7261 aactcgtctg cgaccaaccc ggcatcggcg aaatcaatct gttgcgcccc gccttgaccc
     7321 gcctggctca acgacgcatc gcactggtgc gacccccggg ggaaccttgc acacattgct
     7381 gcaccaactg gcgtctcgac tggcgcaggc tgctctggat caccccccgc tcagccaacg
     7441 acgccctctg ggccgccgaa caaatccttc gccacgacag ccaggcaata cttctgtgct
     7501 gggtcgaccg taccccatca tccgcgctgc gccgcctgca tctggccgcg cgtggcggca
     7561 ccgcactgtt ttttcttcta cgccccgaga ccgcgctgcg gcaacccagt gtggcgccac
     7621 tgcgtctgcg cttgcgtccc gcgatgcaag ggctcgaaat ctccatcatc aagcgccagg
     7681 gtccgctttg cgaccacccc gtatccatcc cgctccaccc acatgcccac gacgccgatc
     7741 atgcccctct ggatctgcct gcacttgccg gaacacagcc tggacgcgtc catcccgggc
     7801 tggcggggta acgccccact ggcggccaca ctgtgcaaag accgtgtcca cacctgctcg
     7861 gcgcaggcca ggcgtctggg cgtacgccct ggcctgcggc tcggcacaac gctggccctg
     7921 gcaccaggta tcgttctggc cgatgacgac cccggcgccc gggacgcatt cctgcggcgg
     7981 atcgcactgg ggttgcaaca gtacacgccg aatatcgcga tccgcggcga acacgccttg
     8041 gttctggacg tgggcgccag cctggcgtta ttccatggcc cccgcgcctt gtggcgggcg
     8101 tttctgtgct cggccaacct gcttgccccc gggttttccg ccggcatggc ccccacatca
     8161 gaaggtgcct tgctgctcgc gctgcgcgaa ccaccaggac gccgccgcgc cctgtgtctt
     8221 gaaaccctgc tccgcctgct cgacgccctg cccgtcggcg tcctgcccgc cgcgcggcct
     8281 tacctcgact ggctcgaagg cataggctgc atcacgctcg cacaactgcg caagttgcct
     8341 cgcaaaggtc tgcgccagcg cgccgggcca gaactgcctg acagcctgga cgccgcctac
     8401 ggccgcctcc agacgcatca tgcctggctc gaaacccccg atctcttttc cctgcgcatc
     8461 gacaccgacc gactcgaaca ccttcacgcc attcaggccg ccgcgacgca actggtggat
     8521 cagctatgcg gctggctgcg ggcaagaaac gccgccgccc aggctctgga attccgcttc
     8581 ttccacgaaa aaggccgcaa cgcggcacca ccgacggtgc tggccatcga actggcccat
     8641 cctcgctggc actccggcga attcaaggac ttgctcatcg agcatctgcg gcgcatgccc
     8701 cccatgccct cgatcatcgg cctcgcactg accattccac gcctcggcac caggccatcc
     8761 cccgaggcgg ggttgttccc cgaactcaac ccggcacaca ctgccgaaca gcagttgctt
     8821 gatcttctat gtgcccgcct gggtgccggg caggtacgcc gcgcccaccc cttggcccat
     8881 catctgcctg aaaaagccaa cgcctgggtt cccgtcacca cccagaacac cgaggcacca
     8941 caccccgcct cggtgttcgc atcggatgcc cgtcctttct ggctgttgac cgccattccc
     9001 ctgaacacgc acaacgaccg ccccgtctac aaaggggcgg cgttgcggct gctgcgcggc
     9061 ccggaacgcc tggccagcga ttggtggagc gattccggcc acgaacagcg cgactatttc
     9121 gtcgcccagg atgcccatgc cgtccgctat tgggtgtatc ggcggcacga cccgtcgggg
     9181 gggcgctggt tcctgcatgg tctgtacggg tgaccgacaa gccccttgat catgtcacct
     9241 cttcctatcc ccccccgcta tgcggaactg gactgcctgt cgaatttctc attcctcaga
     9301 ggcgcctcgc atcccgagga actcattgcc cgcgccgcga agcttggcta tgaagcgctg
     9361 gccctggccg atgactgctc cctggccggc gtcgtccggg cctggggcga ggcccgcgca
     9421 catggcctca agctgatcat gggaagccgg tttcaactgg ccggcgacac gctcggcgcc
     9481 ggcgattcca caacacatga aacggacagc ccgcagctca tcgtcctggc gcgcaacctg
     9541 aacggttatg gcaacctgtc ggaattcatc acccttgccc gatccgccag ccccaaaggc
     9601 gcctatgccg ccggactcga ccttctggcc catccgccag gcaatcaggc tcatctgcgg
     9661 ggtctgcccg attgcctggt cattttccgc ccccgccacg gcaccggacc caaggccctg
     9721 caccagcagc tcgaatcgct actgcctttg ttcaaggggc ggctctggct ggggctcgcc
     9781 ctgcacaacc ggcacgacga cgcacagcac cgggccacgc tggagctcgc cagccggctg
     9841 tacccggtgc cgctcgccgc cattggcggg gtggaaatgc atgcgcggtc ccgccagcct
     9901 ctgcacgaca cgctagccgc catccggctc aagacccccg tgcgcgattg cggctacgcg
     9961 ctgcggccca atgccgagca ccacctgcgc catcgcatgg cgctggcggc gctctacccc
    10021 gccgaggcat tgcgggaaac cctggtcatc agcacgatgt gctcgttcac ccttgatgag
    10081 ctcaagtacc gctaccctga agaagccgtg ccggccggcc acaccgccgc atcctggcta
    10141 cgccaggaaa cctatctcgg cgcggccaaa cgctatccgc aaggcctccg tggcgcactg
    10201 cgcgagcaac tcgacgccga actgaatctc atcaccgaac tgggctatga agcctatttc
    10261 ctcaccattc acgatatcgt ccggttcgcc cggcagcgcg gcatcctgtg ccagggccgc
    10321 ggctcggcgg cgaattcagc ggtctgttac tgcctgggca tcaccgaggt cgatccggaa
    10381 aacggcaacc tgctgttcgc ccgcttcatc agccgcgaac gcaacgagcc ccccgacatc
    10441 gacgttgact tcgaacatga tcggcgcgaa gaggtcattc aatacgttta ccgcaaatac
    10501 ggccgccgcc gcgccgccat cacggccgtg gtctccacgt accggacgcg gggcgcgctg
    10561 cgcgacagcg gcagagcgct gggcctcgac ccgggcctca tcgacagtgt cacggcatcg
    10621 ctgcggtatc gcggcgggtc ccaagccctg ctcgatgagc tggccgcgca tcccgggctt
    10681 ggcggcccta cgcgcactgt gcggctctgg gccgcgctgg cgaatggact gaaaggcttc
    10741 ccgcgtcacc tgtcccagca tccaggcgga ttcgtcatct gcgccgacaa cctgtcgcgg
    10801 cttgtacccg tggaaaacgc cgccatgccc gagcgcagcg tcgtgcaatg ggacaaggac
    10861 gatctcgacg ccatgggaat actcaaagta gacattctgg ccctgggcat gctgtccgcc
    10921 ctgcgccggg cgctggaact gatctcgatc cggcgccaaa ccccgttcac cctgggcgat
    10981 attccgcccc gcgacgaaga cacctatcaa atgatccgcc gggccgacac catcggcgtg
    11041 ttccagatcg agtcgcgggc gcagatgagc atgctgccac gcctggctcc caggagtttc
    11101 tacgatctgg tgatccaggt ctccatcgtg cggccaggcc ccatccaggg cggcatggtt
    11161 catccctacc tgcaacgccg ccagcaaaaa gcacagaacc gccccatccc ctacgccaat
    11221 ccggccatcg aacgcattct ggaacgcacc ctgggtgtgc ccatcttcca ggaacaagtc
    11281 atgcagatcg ccgtgaccgc cgccgacttc accccggggg aagccgacca actgcgacgc
    11341 gccatggcgt cctggcgccg caaaggaggg gtcgataaat tccaccagaa actgctggaa
    11401 ggcatgaaaa agaacggcta cgacgacgcg ttcgcccaat ccatcgtcag gcaactcgaa
    11461 gggttcggcg aatacgggtt cccggaaagc cacgccgccg gtttcgccaa gctggcctat
    11521 tgcagcgcct ggatcaagcg tcacgagccc gaggcgttcc tggtcgcgtt gctgaattcc
    11581 cagcccatgg gtttctactc ggtcaaccag ttgatccagg atgcccgccg ccaccgggtc
    11641 atcatcctac ccatcgacat caatgaaagc gggtgggaca ctgctctgac accgccggcc
    11701 ggcccgggcc gcgcgaccat gtcgacaccg aacgacgaac cacagccagt ggtacgcctg
    11761 ggcctgcatc ttgtcaaagg gctgtcccgg gaagcgggcc aatgtatcga ggcggcccgt
    11821 gtgcaaagtg acttcagcaa tccccaggat ctggcgcaac gggcgcggct caggcgccac
    11881 gacatggact gcctggccaa tgccaatgcc ctggcctcga tcgccggaca ccgtcgccaa
    11941 gcccgctggc aggcggctct gccccggtcc ctcgatctgc tcgaagcctc acccatccgc
    12001 gaaaccgaac agcccgaact ggcggcgcca gggcaaggca tggaaaccat cgccgactat
    12061 gaagccaccc aattcacgct gggcccccat ccactggcgc tgctgcgccc cgcgctgcgg
    12121 gcatcacaat acaatcaagc ccgcctcctg cttgacggct atcccgatcg acgtcgagcg
    12181 cgggcctgcg gcctggtcac catgcgtcag cgaccccaga cagccaaggg cgtcatcttc
    12241 gtcagtctcg aggatgagac cggcaccgtc aacgtcatcg tccgacccga gctggcggcg
    12301 cgtcagcgca ccgaactgct gtgcgcccgc ctgttgggcg tcgacggcgt ctggcaaacc
    12361 caccagggcg tcggccacct gatcgccgag tccctgacca acctcaacac catgctgggt
    12421 ggcctggggg tgcgtagccg tgatttccat tgatcacggg tgccgacaaa gccgttgtgg
    12481 gaagtccggg ttggtcgatg agggggacac agagggtcca tgacgtaggg cctgctccca
    12541 ttgggttctg cacgaaaatg gggtggaacc catacccaca gcgcccataa tagaacaagg
    12601 gcttacagac attcatctgt aagcccttga ttttattggt cgggacggcg ggattcgaac
    12661 tcgcgacccc ttgcacccca tgcaagtgcg ctaccaggct gcgctacgcc ccgaaactgc
    12721 tgctgaaaac ccaccctggt gcatggcccg agctgttttc acaccggcga aacacatggc
    12781 cccgctcagc aaagaagcga attctagcat agtttccgga gccgatccgg gcctccagca
    12841 tgatcactag tgcccagccc cgtccgccac ccgcaaaccc gacgcaacag tgaattttcg
    12901 tcaaagttgt attgtcatca tggtcaattt gcatgctaag ttaaaatttc aggcaaacta
    12961 gggtttacct tgattgtgga ctcaatagca ttatgaccag cttgtttgat ccggttcgtc
    13021 ttggcgctgt ttcgctgaag aaccgtgtcg tcatggcccc catgacgcgc acacgggcgg
    13081 ctcccggacg ggtgcccggc ggcatgatgc gcgaatatta cgagcagcgc gccggcgcgg
    13141 ggttgatttt gaccgaggcc acgtcagtgt cgccgcaagg ggtggggtat ccggatacac
    13201 ccggcatctg gtcccacaag caagtgcaag gctggcagca aatcacgcgt agtgttcatg
    13261 acaagggagg ccggattttc ctgcagctct ggcatgtggg gcgggtatcc gatccggaat
    13321 acctcaatgg ccaaatgccg gtggggccca gtgcgattcg ctgcgagggc tatgtcagcc
    13381 tgatccagcc caagcgcgaa tacgcggtac cgcgcgcact gcatcccgcc gagatacgcg
    13441 cggtcatcgc ggatttcgtc gaaggcgcgc gcaatgccaa agaggcgggg ttcgacgggg
    13501 tcgagatcca cgccgccaat gggtacctga tcgaccagtt cctgcgggac ggcgccaatc
    13561 accgggtcga tgcgtatggc gggggcatcg gcaatcgctc gcgcttcctg ctggagatcg
    13621 tggatggttg catcggcgtg tggggaagtg agcgggttgg cgttcatcta tcgccgcatc
    13681 acgcgctgca ttcggcccgg gattccaacc ccttcgcatt gttcgcccat gtcgcccaca
    13741 cgcttgggga gcgtcggatc gcttttctcg tggtgcggga agccaatgac gagcgtggcc
    13801 tcaccgccac gatcaaacag gaattcggtg gcgccttgat cgtcaatggc ggttacgatc
    13861 tggccctggc ccaggaagcg gtgggcagtg gggccgccga cgcggtggct ttcggcaagg
    13921 cattcatcgc caacccggat cttgtcgtcc ggttgcgcga gggagcgccc ttgaacgtgt
    13981 gcgacacgag cacattttat ggaagcggcc gcaaaggcta cgtggactat ccctcgctcg
    14041 atggcgaatc ggcctgccgg cttgcttgat gcctcccgca cccgacaacg cgcggttcac
    14101 accatcgatc gaaactgttt cgttttgcag gccccgcgta ctaaactgga cccctgccga
    14161 ctgacgacgt gtggcgcggc gactggttcg aaggaacccc aaagcggtcc ctgccattca
    14221 gacaatgggg tttgagcaat gagattcatc cgatgcggca ttgtgttcgc gacgactctg
    14281 gcgctggccg gttgcgcact gggcattaac aaagactcgc cgtcgatatc gtacacagtg
    14341 ccgcgtgatt accgcacggt gtttctgcgg gtgcagaatc aggccgatga atgcctgcgg
    14401 ggcaacggcg cctacacgat ccactcccgg gtggacgccg ccacgcaatc gggcgaggtc
    14461 gatgtcgtcg cgccttttgg cgggacggtt gtcgcccgca ccgtgctgga ggccaccgac
    14521 gccaagcaca cccgcctgac acaaacagtc tgggggcaca aaccctggaa tgccgaggcg
    14581 ctcgacgcca tgcgacagtc ggtgcgcatg gacatgtcgg tctgtttcgc ctaccgctga
    14641 agctcagttc ccggtcaacg cttgcttcac cagggccgaa acccgggtca tgtccacgcg
    14701 gcccgccagt gacgccttca ccaaacccat cactttgccc atggccggtg cgccgccaat
    14761 accctgggcc gcgacctggg ccacggcagc atccactgcg gcccggactt cttcctcgga
    14821 agcttgttgc ggcaggaatt cctgcagaat gcgcagctcg gccttttcag actcggccgt
    14881 ttcgacccgg cccgcctgct cgaaggcggc gatggattcg cgccgctgtt tcacctgctt
    14941 ttcgatgatc gcgatgatat ccgcatcgtc gagatcccgc cgttcgtcca cttctttctg
    15001 tttgacggcc gccagaagaa accgcagcgt cgccaggcgg ggagcgtcct tggcgcgcat
    15061 cgcgtccttg accgagtcgg tcagccgtgt cttgagcgga ttgctcatgg agggttcctg
    15121 aaagtgggcc ggcgccgcaa gcgggcacag cctgctgatg atgagggctc agggccagat
    15181 gccctggatg atcgggcctg gtccaacggg aattttagcg caagggcttg aagtggcccg
    15241 gagtttgtgg gtctcttcgt tttcgctgtt cgccccccac atcccatcgc tgtgcggtac
    15301 gccgcaggct gcctacccga cgacaattga tttgtccaaa cacccgaatc cattccaccg
    15361 gccgcatcct ccggattcag ccacacgtca gcggaacgcg acaatacccc tcgcaaccga
    15421 cgattcgcgc gcctgagccg cgatatacct atttttcccc agccctgatt gatgaatccg
    15481 atttcatcaa tctgtaatgc cacgcacctt cgcagcgcag ggaaaacggc gccaatacag
    15541 gccacgcgct gaattcccca aggccgaccg cgctggcctc atccgaatcc gcacaggcac
    15601 cgatgctgag actgatctgt gttttctcat agagaaaaac accataagta ctaggccata
    15661 tagcctaggt actaggccat tttctagcga acgccctagg ccctcccgcc tatgttttga
    15721 gacaggccag atgcctacca tttcacctac gcaacccgtg tcaaaaagtt aagaaacgca
    15781 tcctggcttg taacacggga aattgaagga agagcgcggc atggcgcctg acgcatttcc
    15841 cttaacaagg ctggtgacag gaggttcaaa gtgttgcggc cccgcataca acttggtgtg
    15901 ctggtgttgg ctctgacggt gcttcaacca gccctggcca aaaccagggc gcccgccaaa
    15961 ccggcgggcc cgaccgttca agggacgaac ctggatgcgg gcagcatcaa caacgagcct
    16021 ctggggggat tgatcatcaa tcgcacgatg actgtcctgg gttttgattt ttatcggagt
    16081 ttttcggaag tctggcaggc ggtgcatcca gattcgaagt tcaacatcac ggtgtatgag
    16141 cgcccaacgg cccagtacgg cagcgaaatc tgggtcagtt accgggatat ccgtgtgtat
    16201 cacacctttc tgtcaccggc gcggtctggc gtcagaaagg aaagcaagct cgcggttgaa
    16261 accgcctaca cgaacattac tgaaatcgat gccgagcgca agcagttcaa aagcgccgac
    16321 ctgggtcccg aggagatgta gatgaaagcc ttcaatacca cgcacatatt ccggcgggtc
    16381 ggcgtcctcg cccctatggc ggcagcgctg gcactggcgg ccgcaccgtc catggcgagc
    16441 cagttgatct acacaccggt taatccatcc tttggtggca accccttgaa cggtacgtat
    16501 ctgctgagca aggcccaggc agaaaacaac catcaggcgc ccctgcctga tgtgggcggc
    16561 ttattgggga acatggggct gaatcccgat ctggccaaca ccggagattt gcagacagca
    16621 acgggcacca ccatcaccgt ccagaatccc ccgatcggtc aacccgtcac cacgcaaatc
    16681 ggctcatccc aaaaataagg tgccgccatg aacgagcgca actttcctcg ccaccgatcc
    16741 acctttcgca ccactcggtt gtgcctgggt ctggtcgcca ccctggcact atcggcatgc
    16801 gccattccgc atccgccgtc aaatgtttcg gctgaagcca agctgacgcc ggcgacgcca
    16861 tccgcgttgg atctgatgcg cctaccgccc cccaagaaga aaatcgtggc cgccgtttat
    16921 ggcttccgtg acgaaaccgg gcaatacaaa ccgtcgcctg acagcgcgtt ctccacagcc
    16981 gtcacacaag gtgcagcgtc catgctgatg aaggccctga gggattcggg ctggtacatc
    17041 cccgtcgagc gtgaaggact gcaatcgttg ctgaccgaac gacgtgtcct gcgcgcggtg
    17101 gatgagcgtg acgccaaaag cggccaaccc gccgctgcca ttcccaacct gctgccggct
    17161 tccatcatct tcgacggcgg catcatcgcg tacgacagca acgtgcgcac aggtggcctg
    17221 ggtgcggaat tcctgggcat cggcatgtcg acgcaataca gcgtggacca ggtcacggtc
    17281 aatctacgcg cggtcgacgt cgacaccggc gagatcctga acagcgtttc cacaaccaag
    17341 accatttatt catacgagat ccatcccagc ctcttcaagt tcgtcaactt ctatgacctg
    17401 ctcgaagtcg agggcggcat cacccacaac gaacccgcgc agttgtgcgt gaaggaagcc
    17461 atagacgcgg ctgtgatgca cctgaccatt cagggcttga aagaccacga atggcagttg
    17521 aaaaacccca gcgactggaa ctcacccatc atccagcggt acctgcgaag cgccagtgac
    17581 tacgcggtca cggtcacgcc gcgcaaggcc atttcactgg atggcgagcc cgctcccgcc
    17641 gtcgagacga agaagccgac tctttgagca cggtgacacc gatctttccg gagacagtca
    17701 tgacccaatt cagaacctcg ctcactcgtg aaaccctctc ccgcttcggg cggcgcggcg
    17761 cctcgctgtg cgcactggtg ctgctgacgg ccagccccct gacgggctac tgcagccccg
    17821 acctggcctc gacatccgag ctgccgccat cggaatacag caacgcgtcg ctgaaggcgt
    17881 cgtccgtcca gtttggctac tacaacacca gccacatcag ccaaacagga gcacgccaga
    17941 cagccgaagt taaccagagt ggcgtggcca ataccgccag catcacgcaa accggcctca
    18001 acgatcaggc catcgccaac caattcggca atggcaattc aacgtttgcc tatcagagcg
    18061 ccagcggcgt cggcgtcatg gtcaatcagt ttggcaacca gaacgaagcc atggtcgtgc
    18121 aaacacaacc aagccctgtg ccgctcgtcg tcaaccagat cggcaatggc tacaggctga
    18181 acgtcattcg atgattcccg caacgctcgg ttccaaggcc gccgggcagc gcattttgta
    18241 caggtcttga aactcatgag gagattcacc atgaaaacca cgctttgcgc aatcgcgtcc
    18301 ggtcttgccc tggccttcgc cggcactgca gcattcgcca ccagcaccgg cagcacgtac
    18361 cagggcggct acaacaacga cgccgaaatt tcccaggcgt acaacgggaa cgccacggcc
    18421 accatcggcc agcttggcgc cctcaacgtt gccaaggcct cccaggacta cactggcaac
    18481 ggcaacgaca tcaccgtgca tcaattcgga gtcaacaact actccaattc gacgcaatcc
    18541 cacaccacga atgccaagtc cacaaccgac cagtatgggt tcgacaacgt tgccaacgtc
    18601 caccaatcat acggcaccaa ccaggacgcc acagccttcc aggtcggctg caacaacacg
    18661 gcgaacatct cccagatggg gtccagtctg aacgccaccg cgatgcaatg gggagtccgg
    18721 aacatcagca ccatcaccca gcgtggttat tgatctcggt acgtagtccg ttcgtacgag
    18781 ctggcgcaca ggaggctctc cggagccgcc tgtgccgcca ggccgacgta caaggaaggc
    18841 acacatcatg atggcggcag acattaacct gcaggtctgg ctcgaaaccg tgccgaacac
    18901 ccaccccagc gtgatcatcc cctacgtgcg gagcaaggaa agcggcaatc ttcattacac
    18961 tctgcgggcc gtcaagcagg gattgggcgg gtcgtcacgc attgctcaaa gcggaggcat
    19021 ccaggttctc gcgaaccaac ctgtggcact ctcgcggttc tccctcagtg tggggaagaa
    19081 tgaccagtgc cgaatcgaac tgatcctggt cgagaacggc aaccccgctg gcacctatca
    19141 tttcgactgc ccgcgctaga ttgacgcctg gaacaaccgc gcagagcgcg attcgatatc
    19201 ccggtcacag aagattttcg ctattcacgt acacaaatta atcgctggca agatataact
    19261 ggaacagaaa tcggtatcac gcttcagcgg agcattcagg aaaacgccac tgcgggctgg
    19321 aagaaatttt ggagggaaat cagacacaat gtgtaataat agaacgaaat tttacggtgg
    19381 ttgttggcga tagcgcgttc caccaacatt caagcgtttc caacgcgtaa cagtgcgcca
    19441 tcgcggcttg aacccgcttg gcagaacgaa ccagccggag ggacgtgacc aagcgctgac
    19501 aacagtgcag cgaagaaccg gtttcagagc cacgggcgac gagaccatga cgggtatcca
    19561 ctcgaccacc cacctactcc accgtcgcgt ctataaatag aaggatgaca accatggcca
    19621 cagtcatcgt ggttgagcca aatgcgcttc ttcgcctggg catactgcag ctggtgcaga
    19681 agatgacatt cgacatcacg ggcgaaggca tcgactatgc ccagttgttc aacggcatgc
    19741 cgaaccagca gggcatggac ctgatgctgc tctcggtacc tgatgcctac gaccgcatgg
    19801 tggaattggt gaacgctgcc caacaaggct attcaccgcg gcgcatactc ctgttgtcgg
    19861 atacgcccat cctcccctat tcgctgctca atcttccccc tgtcctgggc gggtatattt
    19921 ccaagtattc atcgcccgac gtactcaccg cggccatcac cctggtgctg gctggcggaa
    19981 aatgcttccc ctcacccgat ctggccattc atcccgagca acgcgagagc gaatcccacc
    20041 cgcctgctgg tgccgcgccc aggcgccgct ggtacgacaa aggcctgccg ccccccaaac
    20101 tgttgtcggg tgagacccaa ccggacaatc tgcttacgcc ggccgaaccc cgacaaatgc
    20161 gcggcagcga ccatccacca accgtgccgt tcgaagcggg tgaaccccag ccattgtcac
    20221 cctcgctcat cgcgagtgag gccgagatgc tcagcctcac gccccgccag tacgaagtcc
    20281 tggcgctgct ggcccgtggg taccccataa aaagcgtcag ccgggaactg aatatctcgg
    20341 tggccaccac caaggcacac accgaaaccc tgtaccagcg cttgtccgtg cacaaccgga
    20401 atgcggcggt ctacgccgcg ttttcacgcg gcgccacatt ggggtggcgc aaggcggacc
    20461 cggtgaacgg caaacaagcg aaacagccgc gctcctgatc acgccacggc cgcacgtatt
    20521 caccgcacgg cccatttcgg gtcttgaccc tttttacatc agccccattt cgagccgtgc
    20581 cgcttcgctg atcatgtcgc ggctccaggg tgggtcccac accagatcca ccttcacctc
    20641 atccacaccc ggaatggttt gcagcttgcc acgcgcctcg tcggcaatca tggtgcccat
    20701 gccgcagcct ggcgcggtca gcgtcatctc gacatcgacg cgatacagat cattgccggc
    20761 ggctagcacc ttgcaggtat acaccaaccc caggttgacg atgtctatgg gaatttcggg
    20821 gtcgtagcag gtggccaata gtgcccacgc cgcttcttca acggattcgg cagtgactgg
    20881 tccatcaggc acggcgggtt tgtccgggtc ggtgggttcc agccccaggg catcggcatc
    20941 gacgccttcg acccggtaca ggtttccctc gaccagcacc gtatagcttc cgccaagctg
    21001 ctgcgtgatc tgcgcgaaac accctttttc gatggtgacg ggtgatccat acggcaccgt
    21061 gacggccggg cagtcccggc tgaccagcac ttcctgacgt tcgtaacgca tgttcgatct
    21121 cttctattcg gtttgcgcaa cgtcgccggc gctggcgatc gcgttgtgca acgtgtgcca
    21181 ggccagtgtg gcgcacttga ccctggccgg aaattcgcgc acccccgaca acacctcgag
    21241 cttgccgaaa tcccgccctt ccggatgggc atcggtcagc atggcgtgga tgtcgcgaaa
    21301 caacgcttcc acgtcatcga cgggtttgcc cttgacggct tcggtcatga gcgacgccga
    21361 cgcagtggaa atggcgcagc catgcccgac aaaactggca tcggacacga cgccgtcttc
    21421 aaccttgaga taaacggtaa gctgatcgcc gcacaacggg ttgtaaccct cggcgcttcg
    21481 gttggcgtcg gccagttcgt gaaaattgcg cgggttgcga ttgtgatcga agatcagttc
    21541 ctggtagagc tcgcgcagat cgccaaaatg ctcgctcatg cttgtcgccc caccccaaac
    21601 agtttctgag cctggcccag ggcatcgacc agggccgcga cgtcttgctc ggtgttgtac
    21661 accgccaggg atgcgcgcgc cgtgcccgga atgccgaatc gggtcatgac cggcatggcg
    21721 cagtggtgcc ccgcccggat ggccacgccg tgggtatcca gtatggtgcc cagatcgtgc
    21781 gggtgaatgc catcgatgac gaacgacaga atcccggcct tctgtgccgc cgtgccaatg
    21841 agccgcaacc ccgggatggc ggaaacggcg tgcgcggctt gctccagcaa ctcgtgctcg
    21901 tagcgggcga ttgcgtccag gcccaggctg gtgacgtact caatggccgc tgccaacccg
    21961 acgacaccag cgatgttggg cgtgccagcc tcgaaacgct gcggcaccgc cgcgtacgtc
    22021 gtgcgctcga aactgacggt gcggatcatg tcgcccccgc cttgccaggg cggcatggca
    22081 tcgagcacat cgaaccgcgc atacaacgcg cctatgccgg tgggtccgta cagtttgtgg
    22141 ccggagaacg catagaaatc gcaatccagg gcttgcacat ccacagcctg atgcgcaaca
    22201 gcctgtgcgc catcgatgag cacccgggct ccggcggcgc gcgccagacg cgtgatttcc
    22261 gccaccggat tgatcgtgcc caacgcattc gagatatgtg tcaccgcgac aaggcgggtg
    22321 cggtcgccca aaagcgcccg gtattcatcc atcaacaact cgccccggtc attgatggga
    22381 atcactttca gcaccgcgcc ggtttgctgg cagaccaatt gccagggcac gatgttggaa
    22441 tgatgctcca tcgcgctgac gatgatttca tcaccaggct tgagctgcga ccgcccccag
    22501 ctttgcgcga ccagattgat ggcttccgtg gtgccgcgcg tgaacacgat ttcttccgcg
    22561 cgcgcggcgt taaggagggt gcgcacgcgc tcgcgcccgg cttcgtaaag atcagtggcg
    22621 tgctgcgaca gccaatgcac gccacggtgg atgttggcgt tggactgacg atagaaccgc
    22681 tcttccgatt cgatgacgga cgccggcttc tgggtggtgg caccgttatc cagatagacc
    22741 agccgctggc cccgcaccgt ctgggtcaga atggggaagt cggctgcgca atcggcgccg
    22801 ggcgcgcgcc gcacgtgaag atcaggggcg ttcatgacag ttctcccaat tcacgcccac
    22861 cgggcagaag tacctggatg gccgtcgatg cccggcgccg cagtgtttcg tcgtcgatgc
    22921 gggacaaggc ctccgccgcg aaagcataga ccaccagatt gcgcgcatgc gtttcgtcca
    22981 gcccgcgcga gcgcagataa aacaaactgt tctcgtcgat ctggccaacc gtggcgccgt
    23041 gcgcgcactt cacgtcgtca gcgtagattt ccagttcggg ccgggcatcg gcacgcgcca
    23101 tgcgggaaag cagcagactg tcggaacgct ggacggcatc agtgccatcg gcgccaggtg
    23161 acaccaggat gcggcccgtg aacacccccc gcgccgtatc ggccaggatg ccgcgatagt
    23221 attcgcggct ggtgccatgc ggctgcccat gcacgatggt ggtatggtgg tcgacgtggc
    23281 gtcggccatt gacgtaatac aggccgttga gcagggtttc gcaatgctcg ccggagaacc
    23341 ccgtcgcgat gtcatggcgc gccaggcgcg cgccgaacga cagcgaatgc gaccggaaca
    23401 ccgagccgcc cgcttgttcg acatcgagca cgccaagatg gaacgcctgc ggcgattcct
    23461 gttgcagttt caagtgcgtg agatgcgcgt cggctgccac gacggtgcgc gtcaccgtgc
    23521 tggtcaggga tgcctcggcc ccttcaccca gatagtgctc cacaaccgtg gcatgcgcgc
    23581 caggttcggc gagaatcaca ttgcgcacga accccgcgcc attttccgcc gcggccacaa
    23641 acaccaggtg aaccggtgcg tccagctcgc agccacggcc aatgcgcacg aaggcgccgt
    23701 cggaggccag cgccagattc agcgccgcca cgctggaacc atccgactcg gtgcccagac
    23761 aggattcgag ccgctccggg cgctcggcgg cctcgcgcga caacggcccc agctcgaccc
    23821 cttcggggag cgtgccgatg tcggataact caggcgcgtg gcggccatcg acgaagacca
    23881 gccagtggcc ggcgtcgcca tgacgaagct gctctatgcg cgagcgcgta caggtaccca
    23941 ccgcggcggt gaatgacgtc tggcccagcg ccgccaatgt cgtgtgatgc cacttttcct
    24001 gcttggtggt gggccacccc tcggcgacga aacgatccat ggcgcggcgc cgcgtgccag
    24061 ccagccaggg aacagccgcg ccgaacaggc cgtccgcgcg cgcctggaag tcggccgacc
    24121 agggttccgc cgcgctcatg ccttcgcccc tggccgaccc ttgcctttgg gcgcctcggt
    24181 tgcctggggt tcgatccagc catagccacg ctcttcgagc tcgagcgcga gttcccgtcc
    24241 gcccgaacgc acgatgcgcc cgtcggactg cacatgaacg taatcgggaa cgatgtaatc
    24301 aagcagacgc tgatagtgcg tgatgaccag caatgcgcga tcgggtgaac gttggcgatt
    24361 gacgccttcg gccacggcct tcagtgcgtc gatgtccagc cccgagtcgg tttcatccag
    24421 aatggcgagc cgcggctgca acagcgccat ttgcagaatc tcgttgcgtt tcttttcacc
    24481 acccgaaaac ccttcgttca cggagcgata caggaattcc tcgcgcatgc ccaccgcctt
    24541 catttcttcc ttgacgcgct tgatgaaatc catggcgtcg aattccgaca ggccctgatg
    24601 cttgcgcacg gcattgaccg ccgccttcag gaagtaggcg ttcgagacac ctggaatttc
    24661 aatgggatac tgaaacgcca ggaacaggcc ggagcgcgcg cgctcttcga ccgatagcga
    24721 aaacagatcc tgacccagcc aagaggcctc gccctgatca acatggaaat tctcgcggcc
    24781 cgccagcacc tgtgcgagcg tgctttttcc ggaaccgttg gggcccatga tggcgtggac
    24841 ttcgcccgcc ttgatggaga gatccagccc tttcaggatg gccttgtcgc cgatggaggc
    24901 gtgaagattc ttgatatcga gcatgattat ctttgaaggg atttcttaac cgacgctgcc
    24961 ttccaggctg acgttgagca ggttctgcgc ttccacggcg aattccatgg gcagctcttt
    25021 gaagacttcc ttgcagaagc cgttgacgat catggagacg gcgtcttcat tcgacagccc
    25081 gcgctgcatg gcatagaaca attggtcttc accgatgcgc gaagtggtcg cctcgtgttc
    25141 gacctgggcg gaggggtttt gcgcctcgat ggtgggaaac gtgtgcgcgg cgcacttctt
    25201 gccgatgagc agcgaatcgc actgggtgta attgcgcgcg ccctcggcct tgggcgtgac
    25261 ccggaccagg ccgcgaaaca cgttgctgct gcgtccggcc gatataccct tggagatgat
    25321 ggtgctgcgc gtgttgcgcc ccatgtgcgt catcttggaa ccggtatcgg cctgctggaa
    25381 attgttggta accgccacgg aatagaattc gcccacggac tcatcgcccc gcagcaccac
    25441 gctggggtat ttccaggtga tggccgagcc cgtttccacc tgcgtccagg aaatgcgcga
    25501 ccgggggccg cggcattcgc cgcgcttggt cacgaagttg tagattccgc ccttgccgtt
    25561 cttgtcgccc gggtaccagt tctggaccgt ggaatacttg atcttggcgt catcgagcgc
    25621 gatcagttcg accacggccg cgtgcaactg attctcgtcc cgcatgggcg ccgtacagcc
    25681 ttccaggtag ctgaccgacg cgccctcttc agcgatgatc aaggtgcgtt cgaactggcc
    25741 ggtgtcctgc gcgttgatgc ggaaatacgt ggagagctcc atcggacaac gcaccccttt
    25801 ggggatgaac acaaacgacc cgtcggagaa cacggcggaa ttcaacgccg cgtagaaatt
    25861 gtcgccgggc ggcaccaccg aacccaggta cttgcgcacg atatcgggat gttccttgac
    25921 cgcctcggag aatgaacaga atatgacgcc cgcctcggcc agttgcttgc ggaacgtggt
    25981 ggccaccgac acggaatcga acaccgcatc gaccgccacg ccggccagtt tggcgcgttc
    26041 gtgcagcggc acccccagtt tttcgtaggt gcggagaagt tcgggatcga catcggccag
    26101 gctttttgga ccgtctttat tcttcgactt gggtgccgaa taatagcgga tgtcctggaa
    26161 gtcgaccggt gggaattcca ctttggccca atgcggctgc ggcatcgtca accagtgccg
    26221 atacgccgcc agccgccatt cgagcatgaa ttctggctcg ttctttttgg cggacagctg
    26281 acggatgatg tcttcgctga ggcctttggg aagtgtgtcg gattcgattt ccgtgacgaa
    26341 acccggcttg tattcgctcc ccagatagga gtcgatctgt tcagtggccg tgctcattgg
    26401 tgcgtgctcc gtgaaaccat gaatgggctc attggtggaa cccttttgct gcgagcgtga
    26461 cgctcatttg cggatactcc atgaaatggc gctggacgga acctcgcgcc ccccttgtgg
    26521 ctggacaccc gatgtgtcgc gatcaccggt ggtcggccgc atcatgtcgg ccaggctggt
    26581 gtcttcgagg gtgcgccgga cgatggcatt gatgcgctgc cagttgggcc gtatgtggca
    26641 ccccgcctcg aagtagcagg caccaggcgt ggcgctgcat tcggtcagcc cgaacggctg
    26701 ctcttcaagc gcatcgatga tctgggctat actaatttgc tctggtgcgc ggctgagtgc
    26761 atagccgcct ttcgcgcccc ggctgctttt caccaggtcg tgatggccca gggccttcaa
    26821 caccttgctg accacaggct gccccaggcc cagcgtacaa gccagatcgg ccgcgctgaa
    26881 caggcgatcc ggatagctgg ccatgtaggt cagcaccagg gtgccatagt cgatgatctt
    26941 gcttacacgc agcacgggcg gcgtctccag acacgttgca gtgggaatat agtaccgctt
    27001 tagtactgtt tcggtcaagc cggggcctct ggcttatgtc tatcgcaaac cgtttatcca
    27061 tgcggctctc aagggtaatc gggcaccttt cacgaaatgc ctttcacgtc gcatcggccg
    27121 gccggccgcc ggaaccgtag cccgcgccat caccccattc gtcgacatcc atcgcatccg
    27181 gccgcgcact ttcccgcccc gcttcgcgac gcccaggcgt accgtcagac acgcccccac
    27241 cgccctccct cccgatcggg acgccttgtt cccgctcgtc agcctgctgc atgcgccaca
    27301 tctgcgcata gcggccttgc gttcgcagaa gtgcccagtg cgtcccacgc tccagcacgc
    27361 gcccctgatc cagcacgacg atttcgtcag cgtcgacgat ggtcgacaag cgatgcgcaa
    27421 tgatcagcgt cgtgcggtcg cgggcgatct gtcccaactc gcgctgaatg gcgcgttcgg
    27481 tgcgggtatc gagcgcgctg gtggcctcgt caaggatcag gatgggaggg tccttcagca
    27541 acgtgcgggc aatggcgaca cgctgcttct ctccaccaga cagtttcagg ccccgttcac
    27601 ccacctgggt ctggtacccc tcgggcaggc tcatgatgaa atcatgaatg ctggccgcgc
    27661 gggccacccg ctcgacgtcc tcgaaggtcg cgccggtgcg gccataggaa atgttgtaga
    27721 gaatcgtgtc gttgaacaac acggtatcct ggggcacgat gccgatgtga tgccgcaagc
    27781 tgatttgagt cagatcgcgc acgtccgtgc cattgatgcg gatcgccccg gccgtggcat
    27841 cgtaaaaacg gaacaacagc cgcgacaggg tcgacttgcc cgcacccgat gcgcccacca
    27901 cggccaacgt gtgccccgcg gggatggcca ggtccacatc gaacagaatc tgtctgttcg
    27961 cctcgtagcc gaaactcaca cagtcgaacc gcacctcggc ctggcgagta tccaacgcct
    28021 gggcccccgg ccgatcggcc acctcctggt cctgttcgag cagcccgaac atccgctcca
    28081 tgtcggccag ggcgttcttg atctcgcgat agacgaaccc gagaaaattc aggggcgcat
    28141 agagctgggt caggtacgcc gataccagca ccacatcgcc aaccgtcatg gttctatgca
    28201 ccacgcctcg ggccgacaac cacagcagta gcgtgatgcc gatcgtgatg atgatcccct
    28261 gccccatgtt gagcgcgttg agcgacacct gattcttgac ggccgaatcc acccatttcg
    28321 ccaggttccg gtcataccgg cccagttcgt agccctcatt gccgaagtac ttgaccgttt
    28381 cgtaattgat cagggcatcg accgccttgt tgttggccga actgtcgagc gtgttcatgc
    28441 tgcgccgata gaccatgcgc ttttcggtga ccaacaaggt gaagacgatg tagcaggcga
    28501 tggtgcccag cgtgacgacc gcgaacagat agttgtagcg ccacagcaga atggccgtga
    28561 ccatcgtgat ctcgagcagt gtcggcaata tgttgaacac catgaagttc agcaagaagc
    28621 cgatgccctt ggtgccgcgt tcgatgtcgc ggctcagccc gccggtctgg cgctgcatgt
    28681 ggaaccgcat cgacaacaaa agcaggtgtt cgaacacgcg cgcggcaatg cggcggatgg
    28741 aaccctgggt cacccgcgcg aacagggcgt cacgcagttc ggcgaaaaca ctcgtcgcca
    28801 gccgggccag gccgtatccc agcaacgccg ccactggcag ggccaacagt gtggccggca
    28861 cactgagccg gtccacgacg tccttgagga atatgggcag caccacatta gccaccttgg
    28921 ccaacaccag acaggccacg gccaccccga cgcgcgcctt gtattcccag atgtagggca
    28981 acagtgaggc cagcgtgcga aaatcgttgc gcaacctggg agtccggctg aagcggaaaa
    29041 atggcatggt ggatatccgg gaaagccggc aaatgagtga agccgtatag taaaccgcgc
    29101 cgaaccgtgg tcggatcccg ccaacttccg gcagcctggc aatctattgt tacccccaga
    29161 aaaaccatcg gaactttcga gggaggcaac acctaataca ataaccccgc ctcagaggga
    29221 caaaaccgga gattccatgg ctgtttcacc cttcgattat gaagcgacgc tcctcgcttg
    29281 cgccgccggc aagcagaacg cgcttcggga tctattccag catgaagcgc cgcgcatgct
    29341 ggcgctgagc acgaaaatgt tcggccgtcg tgaagaagcg gaagacgcgg tgcgcgacac
    29401 attcatcctg ctctggaaaa acgctggcaa ctacgacgaa tccaccggca ccgccagggc
    29461 ctggatctac agcattctgc gctaccgtgt gctgagccag atacgtcagc aaagccacaa
    29521 tcccggtgac gacaccacct cgatctcgtc gcagaccgac gtcaaggctt gtctgtcgac
    29581 cccggtcaag agcctggccc aattggacga catgcagcgc gcgccactgc tcatggcgtt
    29641 ctatcatggg caaaccttca atcagatggc cgcacggttg atgacgccgg tggagctgat
    29701 tcgcacacaa ctgcgcacgg ggttggatgc ggttcagggg cggcacgcat gagcatagaa
    29761 cgcggctccg aacatgattt cctgatcgcc gagtacacac tgggcctgct cgattcgcac
    29821 gaaagcgccc aggtcaatca attgctcgcc agcgactccc aggctgcatc cacggccctg
    29881 aaatgggagg ggctcttcct ttccctcaca gacgagctcg agcccatcaa ccctcctccc
    29941 caccttctcc agcgcattca agcctcgctg gggcacgaga tatccctggt caagcggcgc
    30001 cgggcatcgc cgggaattct cagaaaaatc gggtccgcca cactgggcgg actatggttc
    30061 tggcgcctcc tcagcgtcgt gctcgccatc acggcggcca cctatgcgct caaccaaaat
    30121 ccaaccgtcg tcgtcgccgc cgacaagcca aaacccaacg tgaccgtcgt caacacgccc
    30181 gcgcccacgc acgtggccat actccaggca ccgggccaga cctccacgcc gggctggatt
    30241 gtcacgctcg acgagaagca cgacctggtc atgaccccgg aagtccacat cgacgtcccg
    30301 accgattccg ccgtgcagct gtggacacac aatccgcgtg atcggacacc gcgctcattg
    30361 ggcattgttc cggcaaacca ggccaccgtc gtgcccgcgg caacgctggg cgacatcgcg
    30421 gacgatcagt tcttcgaaat gacgcaggag caggctggcg gctctccctc tggcgccccc
    30481 acgggcccca ttctgttcct ggggagaatg gttgaactgc ggcccaaacc ggagaaaagc
    30541 aacgggatac aggctgttgg cgcgccagcg tccgcgccgg ccgcgggtgg ccagcccatc
    30601 gacacgaaca cctcagacaa tcagccgaac ggcaagtagc gtcaaagtgg tggcaagggt
    30661 ggcgcgcacg taccgttggg gcaaggcacg ggccaaccgc gcgcccagcc agatccccgg
    30721 cacggaccca agcagcaacg tgccgaggag cgaccagtcc aggtgcccca gccaccaatg
    30781 ccccgccgcg gccaccacgg tcaggggcac cgcgtaggca atgtccgtgc ctgcgacttc
    30841 ggcaggggcc agtttcgggt acagcaggaa aatcaatgtg gcgcctatgg ctccggcgcc
    30901 gatcgaagac agggtgatca gcaccccgag caccgccccg gccgccattg tggccaacac
    30961 ctgggcgcgg ccccgcaacg ggtaggccga acagcggctc aatcgggcca gcaaccgtgt
    31021 gcgcaaggcc agcgccagca ccgtcaacag caccgacaca ccgatgatga cgcgtatggc
    31081 atgcatgaga ccaagactgg ccggccccgc ataacggatg aaaaacaaag tcgccagcgc
    31141 ggcgggcaag cttcccgcgc acaagctggc caccgcacgc caatgcacct ggccatatcg
    31201 gccgtgcgcc agcgtgccaa cgcttttcgt caccgccgcg aaagccagat cggtccccac
    31261 cgcgacaatc ggggtatagc cgaagcccag cgtgagcaat ggtgtcatga gggagcctcc
    31321 tccgacaccg gtgaccccca cgagcagccc caccgcgaag ccgctgatta tccgatacgc
    31381 ttccatgcca tgcatgatag aagcggcacg acccaggccc aacgaataat aaattgatta
    31441 gttatgcacg gcgattacgt cgcgctcagc acaccaccac attgacggcc aacccgccac
    31501 gcgaggtttc cttgtatttg gaacgcatgt cggcacccgt ctggcgcatg gtctcgatga
    31561 cggcgtcgag cgacaccttg tgcgtgccgt cgccgcgcag cgccaggcgc gatgcgttga
    31621 tggccttgac cgcgccaatg gcgttgcgct cgatgcaggg aatctgcacc aggccaccga
    31681 tggggtcgca ggtcagcccc aggttgtgct ccatgccgat ttccgccgcg ttctcgacct
    31741 ggcgcggcgt gccgcccagc gccgccgtca ggccggcggc cgccatggaa caggcggatc
    31801 cgatttcccc ctggcagccc acctcggccc ccgagatcga cgcgttgcgc ttgtacagga
    31861 agcctatggc cgccgcagcg agcaggaatt cgaccatgcc gtctttcgag ctgcctagga
    31921 tgaaatcctg gtagtagcgc aagacggcgg gcaacagtcc ggccgcgcca ttggtgggcg
    31981 ccgtcaccac gcgccccatg gacgcattct cttcattgac cgccatggcg aacaggttga
    32041 cccaatccat cgcgtacatc aggtcgttcc cgggcttgtc acgctgcaat tcgcgataca
    32101 gcgtgggtgc gcgccgtttc acccccaagg ggccgggcag cgggtcatcg gcgtgggggt
    32161 tgtcaatacc gcagccccgg tctatgcatt ggttcatgac gtcggcgata tgccacacac
    32221 cggcgcgcac ctcgtcctcg gacctcagcg ccatttcgtt ggccatggcc agcgcggcca
    32281 ccgacagccc ggtttcggcg cacatggcca gcaactcggc ccccgtggtg aacgggtaag
    32341 gcatgggggc gtgccgcggc acggtttcgt cggtgtcgat ggcatcgagc gcgcggatga
    32401 aaccgccgcc gatggagaca tagcgctcca cgatgatggg ctggcgcgat tcgtcgaacg
    32461 cgctgaaacg catgccattc gggtgctcga ccatgggacg gatgcgatac gacagggctt
    32521 tgccaagatc gaacgcgatg gcgtgctcat gccacagcat gagcgagccg gacgcttcga
    32581 cgcgcgcgac gatggactgg atgtcggcgg gctggaccgt atcaggggcc tcgccgctga
    32641 ggccaagcag gatgccgata tcggtgccgt gcccccgacc ggtttcggcc agcgaaccgt
    32701 acagatcgat cgtcaggcgc gccacacggg agagttcgcc ccggctcttg agattgcgca
    32761 cgaaattgcg cgccgcgcgc atgggcccca ccgtgtggga actcgatggg ccgatcccca
    32821 ccttgaatat gtcaaacacc gagagcgtca tggcgcaagg cctccagttc ctgctcggtg
    32881 aaccccgcgg cgcgccgggc ctgcgtgttg aacggaccac gcagacgggg agcacggtgt
    32941 attaccgcca gtgatcgata ggtcgacagc gggtcgagac ccgctttgtc gcacagataa
    33001 cgataccaaa aattacccac cgcgacatgg cctatttcgt cgtggagaat gatctcgata
    33061 aggttggcgc ccgctgcgtc gcccacggcc tccagtttgt cgcgcacgat gggggaggca
    33121 tccagacccc gggcttccag agtgcgtggc accagcgcca ggcgggccat gatgtcatcc
    33181 cgggtgcgcc aggccatgtc ccacaaccca tcatgggcgg gaaaatcacc atagtcatgg
    33241 cccagggtcc gcagatgccg ccgcaataga ccgaaatggc aagcctcgtc gcgcgcgaca
    33301 cgcaaccagt cagcgtaata ggcatcgggc atgcccggaa agcgccagac ggcgtcgagg
    33361 gcgagattga tcgcattgaa ctcgatgtgc gcgagcgagt gcaccaacgc ggcccgaccc
    33421 tccagcgtat gcacggaacg ttgacgcacc ttgaacggcg ggacccgttc aggcctgatc
    33481 gggcgtcccg gcacaccggg cggctcgtcg agccgcgcac cggcatcgac aggcgacgac
    33541 acggccaggg atcggaccat ggccagcttc tgatcgggat cggccagcaa cagcgcggcc
    33601 agcgcctggg ttcgcatcag aacctgctca tgccgacggg atacgacccg gggtcaggtc
    33661 aaggacgagt caccgagcga ctcggacaac atgtcgcgca cggtttgcag gtcggtccgc
    33721 aacgcctgaa gttcgaccgc gctgaggcgc acggcggcgt cctgatcatg aggggcttcg
    33781 cgggagtctc ccaggcgatt gcgcgcaccg ctgatcgtga agccctgatc gtagagcaag
    33841 tcgcgaatgc ggcggatgag caggacttcg tggtgctggt aataacgacg gttgccacgc
    33901 cgcttgacgg gcttcagctg ggtgaattcc tgttcccagt accgcagaac atgcggcttg
    33961 acgcagcaaa gctcactgac ctcgccgatg gtgaaatacc gcttggcggg aatgggaggc
    34021 agaacgacag gggtttcgct ggagctcatg ggcggtcttg aattgtttcg ccggcaacgc
    34081 ttgtgcgttc gacggtgctc ttgagtttct ggctggcgtg gaacgtgacc acgcgacggg
    34141 ccgcgatggg gatgatctcg cctgtcttgg gattgcgccc cgggcggggc ggcttgtcgc
    34201 gcacctggaa attgccgaat cccgacagct tcacttcgac gccgcgcacc agcgagtcgc
    34261 ggatttcttc gaaaaacgtg tcgacaatgt ctttagcctc acgcttgttc aggccgacgc
    34321 ggtcgaacag gagctcggcc agttcggcct tggtcaatgt gcgggggtca gtcgtcatgg
    34381 gtgccgggtt ctcctcttct gttctgttct gttcacgcac gccgacgggc ggcatgggcg
    34441 tggatcagtg cattcagcaa cttctcgaca cagcgctcga cggtggcgtc gtcgagcgtg
    34501 gtgtctcggc cctgcaacca gaaccggaag gccatgcttt tttcgggggc cccggaagag
    34561 accgacatgt cgcgccatac gtcgaacaag cggaaatccc ggaccacgtc aagagtatta
    34621 tctgaagcga tggcttgtgc aagtgtatcg gacattgcct ggaaaggcac cctggcgtcc
    34681 acccacacag ccagatcgcg cacggcaacg ggttggcgcg ggaagggcgc cagacgggca
    34741 agatcggcac gggagatgcc ttccacatcg agttcgaata cgaccggcgg gtgggtcagg
    34801 tccatctgtt gcgcccagcg cgggtgcagt tccccaagcc agccaatggc gcgcccatca
    34861 agctccagcc gggcggctcg cccagggtgc agcgatggat ggggtgccgc gacacaacac
    34921 agccgcggcg cacgcgcgcc cagcagtgtt tcgacgtctt tcttcacatc atgaaaatcc
    34981 acctggcgca cggcgcatcc ccactgctct tcccgcgccg gcccccacgc ggcgccggcc
    35041 aggcgttgtg gctgaccgac cccgccgacg ctcaatgggc cgtcggccac ctccgcgtcg
    35101 cgcatgaaca cccgccccaa ttcaaagacg cgcacgcgcg tctgcttgcg gttggcgttg
    35161 taacgaatat tggcgaccag gccacccagc aaggacgacc gcatgacggc aagctggctg
    35221 gcgatggggt tgagcaggcg gatggggttg gcgttgccgg cgtagtcacg ctcccaggat
    35281 tcttcaacaa acgagaaatt gatgacttcc tggtaatcca accccgccat cacaccccgt
    35341 agctcgtgcg ggccccgggc ttcctcgggc acagcccgca ttttggccgg cgcacggggt
    35401 ggcacgtcgg ggatacgctc gaaaccgtgg atacgggcca cttcttcgat gaggtcttct
    35461 tcaatggcca gatcgaaacg atacgacggt ggcgtcaccg cgaaaacgcc atcgcgctct
    35521 tcaaaggcca gcccaaggct gctgaagatg cctgccactt cctcgcgcgt gaccggcaca
    35581 cccagaatgc ggtgacagcg cgccagccgc atttccaccg ggcggcgctc gggcagattc
    35641 acgatctgat cgtccacggg gccggcctga ccaccgcaga tttgcaggat cagggccgtc
    35701 atgcgttcga gatcgcgcgg gatggcgtcg aaatcgactc cgcgttcaaa acgatggctg
    35761 gcctcggaac tgaatttgta gcgcctcgcg cgccccatga tggcttcagg ccaccagaac
    35821 gccgcctcta gatacacatt ggtggtgtcg agcgtcacag aggaggcttc cccgcccatg
    35881 atgccggcca ggctctccac cgtgccatcg gcctcgatga tgcccacctg gggatcaagc
    35941 accacggtct ggccattgag cagcaccagc gactcgcctt cgcgcgccca gcgcaccgtc
    36001 agtccccctt tcacgcgatc cagatcgaac acgtgcgtgg gccggcccaa ttcaagcatg
    36061 acgtagttgg aaatgtcgac cagcgccgag atggatcgct gcccggcgcg ttcgaggcgc
    36121 gccctcatcc aggccggtgt ggccgcgcgc gcgttgacac cgcgcaccac ccgacccgcg
    36181 aaacgcccgc acagatcggg cgcctgcacg gaaacaggca atctgtcgtt caaagtgacc
    36241 gatgcccggc cgatatccgg catggtcagc ggcacgctcg tcagggccga gacctcgcgc
    36301 gcgaccccga gaatggagag gcaatcggcg cggttcggtg tgagcttgag ggtgaacacc
    36361 gtatcgtcca gatccagggc gtcgcgcacc gacacgcccg gcaccagatc ggcgtcgagc
    36421 ggcaggaggc ccgcgtggtc ctccgaaagc cccagttcgc gcgccgaaca cagcatgccc
    36481 gccgattcca cgccgcgcat cttcacggcg ccgatggtca cgccccctgg cagcgttgcg
    36541 ccgacccgcg ccagcggaac cttgaggccc gcggcggcat tcggcgcacc acacacaatc
    36601 tgcagggagg ttccggaccc gtcgtccacc tggcagacgc gcagcttgtc ggcattcgga
    36661 tgcggcgcga tatcggtgat cagccccacc accacgccgt tgaagggcgg cgccgcggga
    36721 acggcctctt caacctcgag gccggccatg gtgagccgat gcgacagggt ctcggaatcg
    36781 atatcgggat tggcgaaagt gcgcagccag gattcaggta cttgcataag aatgggacag
    36841 gttcgttgag cagcggtggt gccgcgacat taccggttga actggctgag aaaacgcaaa
    36901 tcgccttcga agaactggcg cagatcgtcc acgccatagc gaagcatggt caggcgctcg
    36961 aggcccgatc cgaatgcgaa accgatatag cgttccggat cgaggccgaa attgcgcacc
    37021 acctgtggat gaacctggcc ggagcccgaa atctcgagcc agcgcccgcg attgggcccg
    37081 gaggtgaaca tcatgtcgac ttcggcggaa ggttcggtga acggaaagaa cgatggccgg
    37141 aaccgcaccg acagatcgtc ggtttcaaaa aacgcccgca ggaaatccgt gtagacgcct
    37201 ttgaggtcgg cgaaggaaat gtcgtcggaa atccacagcc cttcgacctg atggaacatg
    37261 ggcgaatgcg tggcgtcgct gtcgacacga tacgtgcgcc ccggcgcgat gaccttgatg
    37321 ggcggcgtgt tcatgcgcgc gtaccgcacc tgcatggggc tggtgtgggt gcgcagcacc
    37381 aacggcagcc cctgcgcgtc gttcatgtcc acatagaacg tgtcctgcat ggagcgcgcc
    37441 ggatggttct cgggattgtt cagggctgtg aaattggtcc agtcgttctc gatctcgggg
    37501 ccatcggcca catcgaatcc gatggaccgg aagatggcct cgacgcgttg ccaggtctgg
    37561 atgaccggat gaatgcctcc cctgccccgg ccacggccgg gcagggtcac atcaaccgtt
    37621 tccgccgcca gcctacgatc gagctcagcc tgggccaaat ccgcgcggcg ttggtcgagc
    37681 aaggcttcaa cccgctgctt gagctggttg atgcgggcgc cctcggcgcg cttctgttcc
    37741 gggtccagcc gcgccaaccc ttttagcagc gcggtcaggg cgccctgcct gcccaggaac
    37801 cgggccttgg cattctccag cgccgcggcg tcggacgcct gcgcgaactg ttctcgtgcc
    37861 tgatcgatca ggtcatctga agctgcaatc atgaatccgc tttccccaaa tgcaaacgga
    37921 gcccgtcaga gccccgtttc accatcatat cgaaccagaa cgggttacga agccaaggca
    37981 gacttggcct gcgcaaccac ggccgcgaaa ccggccttgt catgcacggc catgtcggcc
    38041 agcaccttgc gatcgagctc gatggaggcc ttcttcaggc cggcgatgaa tacgctgtat
    38101 gtgacgtcga gctcacggca cgcggcgttg atacgcgtga tccagagcgc gcggaacgtg
    38161 cgcttcttgt tgcggcggtc gcggtaggca tactgcccgg cgcgcatgac cgcttgtttg
    38221 gcgatgcgaa agacgttgcc gcggcgaccg cggtagcctt tggctgcatt aatgactttt
    38281 ttgtgacggg cacgggcggt aacgccgcgt tttacgcgtg gcatggtggt gggtctccga
    38341 taatcaagcg aaaggcatca tggccttaac cgaggcgaca tcagagtcgt gcacggcggc
    38401 cgagccgcgc aactggcgct tgctcttggt ggttttcttg gtgaggatat ggcgcttgaa
    38461 cgcctggccc cgcttgattg acccgctcgc acggaccttg aagcgcttcg cggcgccccg
    38521 cttggttttc atcttgggca tggttttgct ctagagtttt tttcgtgaag gaaggtggcg
    38581 cactcgatct accggccgca cttgtactac ccagcaatca cgtcaatgtc gcgccatcgg
    38641 gggcatgcct cgcagccgca cccccgctct tgccacagct ttgtctgcca gaaaaggaaa
    38701 gaaagccatt gattatatga tgattttcac cgacatgtcc agcgacggca cctcatgatc
    38761 aacgccatgg ctgccgatat tgaatagcct cggcaacatg cgtgccgccc accaaatggg
    38821 catcatccag gtcagccacg gtgcgcgcaa cgcgcaatac acgatgcacc acgcgcgcgg
    38881 accagttcca gcgcgacatg gcctgacgga gtatcacctt cgccgccgtg tcgggttgac
    38941 aatgcgcgtc gatgccagcc accccaagca gcgcattggg caccccctgg cggaccagtt
    39001 ggcgccgccg gcacgcctcg accctgatcc gcacaggcat ggaggcttcg cccgccgggg
    39061 catccatcca atgggcatcc gccgaaggca gcgaaatctg cagatcgatg cgatcgagca
    39121 gtggccccga cagcctcccc cgatattttt caatgcggtc cggcgtacag gcgcagcgca
    39181 tgtgcgggtg gccatgccac ccacagggac agggattcat agcggccacc agttgaaacg
    39241 cggcgggaaa tgtcagagac cgcgcggccc gcgcaatgga cacacagccc gtttccagcg
    39301 gctcacgcaa tgcttccaga gcgcggcgat cgaattccgg tagttcgtcc aggaacagca
    39361 cacctcggtg ggcgaggctg atctcgccag gcctggggtg gattcctccg ccgacgagag
    39421 ccgggacgga ggccgaatgg tgcggcgcgc gaaaaggtgg cacatcggtg aaacggtatt
    39481 ctcggccgcc aatgccggcc agcgccgcga cttccaggac ctggtcctgg ccaagacgcg
    39541 gaagcaggcc cggcaggcga tgggccaaca tacttttccc cgtgcctggc gggccggcca
    39601 tgagcaggct gtgggcaccc gcggcggcga cttcgagcac ccggcgcgcc agcgcttgac
    39661 cccgtacgtc ggataaacag ggcgccgttg atccgtctgt ggaaatggtc cgcggcgccg
    39721 ccatggccag gcgctcaccg ccactgagat gtgcaaccac ttgcgcgagg ctgcttgccg
    39781 ccaggacgga caaccccggt acgctcgcgg cggtgccggc cgacgcggcc ggcaacacca
    39841 attgcgcatg agggtgctcg cgcgccacgg ccagcgcaat ggcgagcggc gctccaaccg
    39901 ccacgaccgc ccctgtcagg gacagctcac ccgcgaaaac atgattcgcc agcgtgagtg
    39961 cgttgtttcc ctcgcccgca ccatcgatgc cgggcggggc gggcgccacc acgggaattt
    40021 ggccggaggc aagcagaacg cccagggcga tgggcaaatc gaagcgcccg gattccttgg
    40081 ggagatcggc gggcgccaga tttaccgtga tacggccggc agggaattcg aacccgctgc
    40141 tcaacagtgc cgaccgcacc cgttcacggc tctcacgcac ccccgcgtca ggcagcccca
    40201 ccacgtgaaa cgctggcagc ccggaagaaa catgcgtctc gacccgcacg gacacggcct
    40261 ccagcccgca cagcgcacgg cttgcaagta ccgccaacga catcgtcata cctcatgatg
    40321 ggtcggcgcc ggatacggcg cccggtatga cagcatgcca cgctgcggcg ccgcccgaga
    40381 cgtgatgcgc cgcattcgtc cattgcggga atcccccgca ctccgggtgc ggatcagttt
    40441 ccgtgtcggg cttcgagttc ctggactttc gcttccaggg acacgatacg cgccatcgcc
    40501 cggttcagca gatcagcctg cacatcgaat tcttcacgcg tgatcaagtc catttttgaa
    40561 aacgtctggg tcatcatggc tttgatgttg cgctcgatat cggcggccgg actcttcgcg
    40621 atcaggtcgg acaaattctt ctgaaaatcc tcgaaccagt ctgcgcggtt gaccatggtg
    40681 tgctcccaac aagttgacat ggtcatagtg taatgaaaac accctggaag ctggcttagt
    40741 gattattggc cgacggggtg gatggcgtgg cggtaccgtt gccgaccgcg ccctggatct
    40801 tctgattcgc ttccgccgcg cccttcttga tcgcgtgtcc catgctgtcg agtgtttcag
    40861 ccgcgctgtt tttcagctcc ccggcctttt gcgccacggt gtcggcggcg ctgttggctt
    40921 tgctcatggt gttggaatcg ctggaggagg acgacgaagg cgccggcgcc ggctgcgtgg
    40981 acggtgcggg ggtcggcgtg ctttccgggg cggccggctg cgtggcggtt ggggcgccgc
    41041 tgctcgtgac gctggcatcg tcgctcttcg aacaagccgc gagcaggctc aacgacgccg
    41101 cgatggcgaa gacggcaaag tatttgtgcg tattctgcat ggaatccctc tcaagttcgt
    41161 gttgtggcaa gactgcttgc cggatcgatc catcgatggc ggggctctac aacaccacac
    41221 caactgaatc cattctatcg acacgtcagc gacgccatac cgggcagatg taaaccctga
    41281 tttcacaggg atattgggca gaatcgcaaa ttccgggaaa acccttattt cgcatcggga
    41341 tgccaggttg ccgagaggcc gggcaatgtc gaccgaggaa acaattgccg ccttttctac
    41401 aaccatggtt acatctttcg ggccgaaacc gcgggaatca gcccgcgagc gccgccagat
    41461 cgatacgttc ggcacgattg agggccgacg ccaccttgac gcgcagcgca ttggaatgcg
    41521 cccgccggat ctctttttta acgtccagca actgctgcgg atgcatggaa aactctttca
    41581 accccagccc cagcaacatc cgcgtcatgc tggcgtcccc agccatctcg ccacacaccg
    41641 cgaccggctt gccggcgcgc tcgccgacat tgatggtgtt ggcgatcagg cgcaggacgg
    41701 ccggatgcat cgggtcgtac agatcgccca catcgctgtc gctgcgatcg atggccaggg
    41761 tgtactggat gaggtcgttg gtgccgatgg acaggaaatc caggcattcg acgaacggat
    41821 cgatggcgat ggcgatagcg ggaacctcga ccatggcgcc cagcgcgatg tcgcgcgaat
    41881 acgcgacgcc gcgtgcgtcg agttcgtgcc aggccgcctc gagcgcctgg cgtgtggcga
    41941 tcacctcgcg catgtgggac accatgggga ccaggatgcg gatgggcccg tgttgcgccg
    42001 cccgcaacaa ggcccgcagc tgcgtgccga acagctctgg attcgccagg caatagcgaa
    42061 tggcccgcaa ccccagcgcc gggttggtgg cgacggtggc ctcgccatcg agggttttgt
    42121 cggaccccag atcaagcgtc cgtatcgtga ccgggcgccc cgccatgaca cgcacgacgg
    42181 acgcataggc ctgatattgc tcttcctcgc cgggcagatc ggcgcggccc atgaacagga
    42241 actcgctgcg aaacagccca atgccctcgg cgccggcctg cagtgcggcc gccgcttcct
    42301 cgggaagctc gatgttggca tgcaggccga tatgaatgcc gtccagcgtg acggccggcg
    42361 catcgcgcaa caaggcaagc tcggcgcgct cttccgcgaa ttccgcctga cgccggtggt
    42421 attcatcgag caccgccgcc gagggattga ccagcaccat gccgttggcc ccatcggcta
    42481 tgagcacgtc gccatcccgc acgagggttc gaacattgcc caggccgaca acagcgggca
    42541 cgttcatgct ccgcgcgacg atcgcggtgt gcgacgtggc accgcccaga tcggtgagaa
    42601 acgccccgaa tcgtccgccg cgcagacgca gcatatcggc cggcgagata tcccgggcaa
    42661 cgacgatcaa cgacccgtcg gactgcgtga gattgggtac gacggcgttg agcgtcggca
    42721 acagcgccga tgaccccgac agcacacgca gcacgcgttc aatcacttgc cggatgtcac
    42781 cgccgcgctc gcgcaaatac tcatcttcta tggcggaaaa ttgctcgccc agaatctgcc
    42841 cttgcgtggt cagcgcccat tccgcgttgt actggcgctg cgcgatgagt tcacacgtct
    42901 gctcggacag cgtgggatcg tcaagcagca tgctgtgaac cgtcaacagc gcacccagct
    42961 ctcgcggcgc gtcgtcgggc agactgccaa ccatgtcctg gagctcacgg ctggctgtgg
    43021 cgagcgccgc cctcaggcgg tcgctttcgg cctggatgtc atccggctgt atgcggtaat
    43081 gcgccacctc gagcgccgcc gcgctcatga ccacggcgcg gccgatggct atccccttga
    43141 ccaccccctg cccctgcaga caaagcatgg cattgagcgc caaagaatcg gaagcatcca
    43201 ccggcatcat gaccctctct gtaggcacaa ccaaaaccgc gttattcctg ctcgccgaac
    43261 ttgttgctga acaaggcctc gatctgcgaa agggcttggt ccgcgtcttg cccggacgct
    43321 tcgatcttca ccgtgacgcc cagccccgcc gccagcatca tcacccccat gatgcttttg
    43381 gcattgacgc gctgagcgcc ccgcgcgatg aagatttcgc tcttgaattg acttgcaagc
    43441 tgcgtgagct tggcggccgc cctggcatgc agccccaatt tgttctgtac aacgatgtcg
    43501 gtgattggca tggtgctgtt cttacgaagc taaaacaaga atcggcatct ggctgtcggt
    43561 cagcacttgt gatcggcatc aataatgcct ttgagcgcac ccagacgaac cttctcggta
    43621 agtttatccg gattttcctg gcgttcggtg agagccttca gcaccatgca gagattcgtg
    43681 ccggtcacca ggtgcccgcg tccgccccgt tgcgtggcga cacgcagcgc ctggctggcg
    43741 atattgaatg gcgtggcacc aaagagatcg cacaggatca gcaccggctc gtcgggccgc
    43801 gacgcgatga catcggcgat ttcctcggtt ttccgggcgg gttcatcggt gggcaggatg
    43861 tccaccaccg tcaggcccgg cacatgcccc aacacatgct cggcgcattg gaaaaaagcg
    43921 ctgcccagcg gggcatgcat gatgaggatg atccgcacca tggttagata cccacggcgc
    43981 gttccagcgc cgacacaaag aagcccgcca cgtcgaaacc cgtttgctcg gtgatttcga
    44041 cgaaacacgt cgggctggtg acgttgattt ccgtcacata ctggccgatg atgtccaggc
    44101 ccaccagcaa gaggccgcgc cgcgccagcc taggccccac caccgcggcg atctctttgt
    44161 cgtgatcaga cagcggcctg gcgacgccac gcccgcccgc agccagattg ccgcgtgttt
    44221 caccggcgag cgggatgcgc gccagcgcgt acggcacagc ttccccgcct acgatcaata
    44281 tgcgtttgtc gccgtcgacg atctcgggaa tataacgttg cgccatgatg gttcgcgaac
    44341 cattcgcggt gagcgtctcg aggatgacgc cgagattggg ttcttccggc cgcacgcgga
    44401 aaatgccggt gccccccatg ccatccagcg gtttcacgat gacatcgccc tggcgcgcgt
    44461 ggaacgcgcg caaccgggac atgtcggacg tcaccagggt gggaacggtg aattcggaga
    44521 attccgtgat ggcgagtttt tcgggatgat tgcgaatgcc agccccggaa ttgaataccc
    44581 gcgcgccttg agcctcggcg tattccagca tgtgcgtcgc gtagatgtac tccatatcga
    44641 atggaggatc cttgcgcatg atcactgcat cgaacgcgcc cagatcggtg tcggccgccg
    44701 gcactgtttc gttccaccag ccgtgtccat gcaggtcggc atccgggcgc agggcgatgc
    44761 cggctgcccg ggccttgacc acgccttcat cgatatacag cccgcccatc atggccacgc
    44821 tgaacgtgtg gccacgatgg gcgagcgcac gcatcatcgc gaccgacgag tccttgtagg
    44881 ccttgagcaa gggcagcgga tcaataatga acaatacgtg cataggacgc ctgctggctg
    44941 cgccccgccc ggcggacgcc gggcgggaac ggattgactg gctggatcag gaagccgaat
    45001 tggtctgctt gccgggagcc ggttgtttct tgcgaggggc cagaaccatc accatctggc
    45061 gtccttcgag cttgggcatg gcttcgacct gacagagttc aaccagatcg tcgcgcacgc
    45121 gctgaagtac gcgcatgccg agctcctggt gcgccatttc gcgcccacgg aaccgcaacg
    45181 tgaccttggc cttgtcgccc tcttcgatga agcggcgcag attgcgcagc ttgacctgat
    45241 agtcgccctc atcggtgccg ggacggaact tgacttcctt gacctggatg accttctgct
    45301 tcgcccgggc ttcctgctgg cgtttttgct cttgatactt gaacttgccg tagtccatca
    45361 ggcgacagac cggcggctcg gcgttcgggg cgatttcgac cagatccact tcgtgctgct
    45421 ctgataaacg aagagcttcc gaggtttgga cgatgcctag ctgttccccg tccacgccta
    45481 tgagtcgcac ctccggtacg tggatttcgc cgttgatgcg attgggttta tcggttgcga
    45541 tgtctgaaac tcctagaatg aatgaatgtg atgcgcacgg ctggcttacc gtcgcgcgtc
    45601 gatttcctga cgcagatgct gggtgaaatc agccaacacc atggcaccga ggtcggcgcc
    45661 gccacgctgg cgcaccgcga ccgtgccggc ttcccgctct ttttcgccca ccaccagaat
    45721 atacggtatc ttttgcatac tgtgctctct gattttacgg gtgatctttt caccgcgcaa
    45781 atccgcgctg gctctaaacc cttgtttttt cagggttgcg acaacctcgg cggcgtattc
    45841 ggccgaatgt tcggaaatac agcacaccac ggcgtgttcc ggcgccagcc acgcgggcat
    45901 ggcaccagcg tggtgttcga tcagcatacc gatgaagcgt tcgagcgaac ccagtatcgc
    45961 ccgatgcagc atcaccggcg tacagcgctg gtcgttggca tcgacgtact cggcaccaag
    46021 ccgctggggc atggaaaagt ccacctgcat ggtgccgcat tgccactgcc gcccgatcgc
    46081 gtcgcgaagc gtgtattcga tcttcggacc gtagaaggcc ccttcacccg gtgacaccac
    46141 gtaatcgcaa cccgtgcgct cgaggctttc catcaaggcg cgctcggcct tgtcccagac
    46201 ctcatcgctg cctatgcgct tatcgggccg cgtggcgatc ttgtacagaa tgtcggtgaa
    46261 accaaaatcg gcatacacct tctgcagcaa cagcgtgtag accgcgcatt cttcctggag
    46321 ttgatcctcg gtgcagaaaa tatggccgtc atcctgcgtg aagccgcgca cccgcatcaa
    46381 cccgtgcagc gaaccggaag gttcgttgcg atggcactgg ccgaattcgc catagcgcac
    46441 cggcaggtcg cgatacgaac gcagccccgc cttgaagatc tgcacatggc ccggacagtt
    46501 catcggcttg aggccgtaga cacggttttc cgactccgtc gtgaacatgt tctcggcgta
    46561 gttgtcccaa tggccggttt tcttccagag ggacaggtca aggatttgcg gcgccttcac
    46621 ttcttcgtag ccgttgtcac gatagatcgt gcgcatgtat tgctcgacct gctgccacag
    46681 cgtccaaccc ttggggtgcc agaatatcaa ccctggcgcc tcgtcctgga aatggtagag
    46741 gtcgagctcg cggccaagtt tgcggtggtc gcgcttctct gcctcttcga gcatggtgag
    46801 gtaggcgctc tgctcttcct tggtggccca cgccgtgccg tagatgcgct ggagcatttc
    46861 gttgttgctg tcgccgcgcc aataggcacc cgcgaccttc atcaacttga acaccttgag
    46921 ccggccggtg gacggcacgt gggggccgcg gcaaaggtcg atgaaatccc cttcgcgata
    46981 caggctgatg gtctggttgg ccggaatcga ggcgatgatc tcggccttgt agtgttcgcc
    47041 ctgcccttca aagaacttga ccgcgtcatc acggccccat tcctcgcgcg tgacgggttc
    47101 gtccttgcgc gccagttcgg ccatcttgtt ttcgatggcc tccaggtctt ccggcgtgaa
    47161 cggacgtttg aaggaaaaat cgtagtagaa gccgttttcg atcaccgggc cgatggtgac
    47221 ctgggcgtcg ggaaacaagg acttgacggc gtaagccaac aggtgggccg tcgagtggcg
    47281 aatgaggtcc aggccgtcgg catctttggc ggtgatgatg gccagcgacg cgtcgcggtc
    47341 aatgcggaaa ctggtgtcca ccagtttctg agcctcgcca tcgaacccca ctttgcccgc
    47401 caacgcggcg cgtccaagcc cggcgcctat cgacgacgcg acatcaccga ccgatacggg
    47461 atcggaatat tcgcgtacgg aaccgtcggg caaggtgatg tgaaccatga aaaatcctga
    47521 aaaaacaaaa acgcggccat gaaagccgcg tctgggggta ttcggtattg ctgatcagcc
    47581 agagacaatg cgcggcaacg gctagagaaa ccaagtggtc gttgttcgcg tggcggttgt
    47641 cgtcatggct gtcgggctga gctgggcgat gcgttgaaat ggggatctgg atacgtgtgc
    47701 cagtgtaaca tcggccgcgc cgcaatgctc gagaaacagg gggaaagccg cgcatcgtca
    47761 acctgatggc ttcgggactg ttgcaaaaaa gcgctatccc ggcgtacaca caagaaaaaa
    47821 ccgccacccg gaaacgggta cggcggttgt tctcaaaggc aaggctggcc tgccggatgc
    47881 agaaaactgg taggcagtac tgggttcgaa ccagcgacct ctacgatgtc aacgtagcgc
    47941 tctaaccaac tgagctaacc gcctagaaag agcaagatta tagtgcgaat gatgccacgc
    48001 tgtcaaaacg aagcaggcaa cgcgcaagct acaggcgtaa gcatggcgtg gcatcacaat
    48061 gcggcatagc gctaatatcc gggagactga tatccaggtg atctattcat cgaatttccg
    48121 attcaggaca ttgggctgcg tttgattcag gtcggccgcc gcaggctgtc gtgctcgcgc
    48181 acgcccccga tgaacgcagc gacgcgctcg tcggacttga tgcccgggga gtcctcgacc
    48241 ccgctgctga cgtccacggc gaaaggatgg accgcatcga gcgccgtgcc cagcgtcgcg
    48301 gcggacagcc cccccgcaag aatgacgggg cgcgcctgct cgcaggcgag cacatcgcgc
    48361 aaaagcgaaa cgtcgaatgc gcggccgctt ccaccaaacc cactggaaaa actgtcgaac
    48421 agccaaccgg ccgcatcgtc ataacgccgg cactgggcca acagaccttc cggcgtggcc
    48481 agcccgggcg cgccaacacg gaacgcccgg atgtagcggt gcgcgaaccg gcgacagaat
    48541 tcaggggttt cgttgccgtg gaattgcaga aggtcggggc gcacctgatc gatgacacga
    48601 ttcacctctt cgacggtcgg atccacgaac aaggcgacgg cgctgacgaa agcgggcacc
    48661 gtttggcgca gggcccgggc ctgttccacg gacaccacac gg
//
LOCUS       DASTNK010000183        18798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_732932, whole genome shotgun sequence.
ACCESSION   DASTNK010000183 DASTNK010000000
VERSION     DASTNK010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 18798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18798
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732932"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..1594)
                     /gene="lpdA"
                     /locus_tag="VFR20_13410"
     CDS             complement(185..1594)
                     /gene="lpdA"
                     /locus_tag="VFR20_13410"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU0231559.1"
                     /translation="MSAQSTTLLVIGGGPGGYVAAIRAAQLGIDTVLVEGATPGGTCL
                     NIGCIPSKALIHAAHEFEKVAHYAKGSALGITVGSPGLDVTRTVAWKDGIVRRLTSGV
                     AGLLKKHGVRVVRGWAEIKDGKTVDVRPVDADAPEGVQTFQCRHMLLAAGSEAVELPF
                     MPFGGRVISSTEALSPARIPDRLVVVGAGYIGLELGMVYRKLGADVQVVEAVDRILPA
                     YDAELTQPVLASLRKQGVGLHLGCSVQGLTATGDAVRVRAADGTETELAADQFLVAVG
                     RRPRIKGWGLEKLMLDMDGRAIQVDERCHTSMHNVWAIGDLTGEPMLAHRAMAQGETV
                     AEIIAGKPRTFSPASIPAVCFTDPEIATAGLGPDEAKQAGLDIVTASFPFAANGRAMT
                     LESTEGFVRVVARADNHLILGWQAVGGGVSELSAAFSQSIEMNARLEDVAGTIHAHPS
                     LSEAVQEAALRALGHALHI"
     gene            complement(1599..3179)
                     /locus_tag="VFR20_13415"
     CDS             complement(1599..3179)
                     /locus_tag="VFR20_13415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEU0231560.1"
                     /translation="MGIHIIKMPDIGEGIAEVELVEWHVQPGDDVAEDQLLADVMTDK
                     ATVEVPSPVVGKVLSLGGQPGDVMAVGAELIRIEVEGAGNAAAAGHAPESRHDAPTGT
                     AGRTAPASTTAGSSGDGHVVSQAASAAASGPANTSGAVTPANPSPVQSDAPASGTGGV
                     PASSQASTLTSRISTSTSTASVPASSASIPTLRTSGAAARPAAAASVVASVSKPAEAS
                     VAKPLSMAPSGALRQPGEKPLASPAVRQRAADQGIDLRFVPATGKAGQITQDDLDTYA
                     ARGATVVSPAPASGYAERTGEHAVPVIGLRRRIAQKMQEAKRRIPHFSYVEEVDVTEL
                     ETLRVRLNARFAAQRGKLTLLPFLIRAVVLAVRDFPQVNARFDDEAGVVTQYGPVHLG
                     VAAQTEAGLMVPVLRHAETHDLWSCARQVARLADEARTGKVSRDEVSGSTITITSLGA
                     LGGIVSTPVINHPEVAIVGVNRIVERPVILGGAVVARKMMNLSSSFDHRVVDGMQAAE
                     FIQVVRGYLECPATLFVE"
     gene            complement(3258..4298)
                     /locus_tag="VFR20_13420"
     CDS             complement(3258..4298)
                     /locus_tag="VFR20_13420"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU0231561.1"
                     /translation="MANDTNDNIVSMTMIQALRSAMDVMLGSDENVVVFGEDVGYFGG
                     VFRCTEGLQAKYGKHRVFDAPISEGGIVGAAVGMGAYGLRPVVEIQFADYFYPASDQI
                     VSEAARLRYRSAGDFISPLTIRMPCGGGIYGGQTHSQSPEALFTHVCGLRTVIPSNPY
                     DAKGLLISAIECDDPVIFLEPKRLYNGPFDGHHDRPVVPWSKHPKGQVPEGYYTVPLE
                     SASVVRPGNDLTIITYGTMVFVSEAAVQETGIDAEIIDLRSIWPLDFDTIINSVKKTG
                     RCVVVHEATRTSGFGAELVALVQEHCFYHLEAPVERVTGWDTPYPHAHEWAYFPDPTR
                     VGAALRRVMDAG"
     gene            complement(4301..5533)
                     /locus_tag="VFR20_13425"
     CDS             complement(4301..5533)
                     /locus_tag="VFR20_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase
                     (2-methylpropanoyl-transferring) subunit alpha"
                     /protein_id="HEU0231562.1"
                     /translation="MTQDIPLRLHVPEPSGRPGQPTDFSFLQLAPAGAARRPPVDVSP
                     LDISDLAYSLVRVLDDDGNAVGPWAPDIDPAMLRHAMRSMIKTRVFDARMLIAQRQKK
                     MSFYMQSLGEEAIGTAQILALRKGDMCFPSYRQQSLLIAQDMSLVNMMCQLFSNDLDH
                     LKGRQLPVLYSYPDAGFFTLSGNLATQFVQAVGWAMASAIKGDTRIASAWIGDGATAE
                     GDFQTALTFAHVYRAPVILNVINNQWAISTFQSIAGGEGTTFAARGLGNNIASLRVDG
                     NDFLAAYAVSQWAAERARSNAGPTLIEWVTYRAGPHSTSDDPSKYRPADDWAHFPLGD
                     PIPRLKQHMIKLDLWSDDQHEAAQKEIEAEVIAAQKVAEGHGTLVGGHMSSVSSMFED
                     VYKDMPEHLRRQRQQLGV"
     gene            complement(5832..8375)
                     /locus_tag="VFR20_13430"
     CDS             complement(5832..8375)
                     /locus_tag="VFR20_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEU0231563.1"
                     /translation="MRGRFCLLTAVAASGAVHSLSVLPGRVSLVWTGIALVIVSCVAM
                     AARCLCRHGWSVPIRLSGGAASRCRVATTIMLVLWVAYIAFASTVWRAQALLDDRLAP
                     DNENKVSRVVLRIATLPRLSPDRRQFEAEVLSSRPMGVPSRILVSWSLGKWGGPYGSG
                     DSRPADFPDLIPGQIWRMALTLKVPHGERNPHGFDYEGHLFARGIRATGSVRGTPRYL
                     RDEPWASLPVVAERARHVVRKAMRPYLEGRRYGAVQLALAIGDQDSVPSGDWTIFNVT
                     GITHLVSISGSHITMIAALGGVLALRLWPLTRLGGRALAERVPAQVAGAMAALLVAWV
                     YCLLAGWGVPARRTFVMLAVLACARAARIPLGVSRALCLVLFVVVALDPWAVLASGFW
                     LSFGAVGILLASGGWSGHPMTRSTPSRWDRFRQSVGLATRLQLAITAGLTPILAGVFH
                     QVSVISPLVNAYAIPAVELLVTPLSLLTAAAALVPGLDWLAGAFAWLGHGVLASVMAP
                     TVWLAGSPVATFDAAAPPLWVLVSASAGLVIAILSWGFRDGRIAWLLLIPAVWWRADR
                     PGQGEWRLAALDVGQAGAVVVQTARHALVFDTGLRTGPDSDSGARTILPYLRATGVRR
                     IDALVVSHADIDHAGGARSLLSALPVMQSYSSFDLAAYLRREARLLGRPDDLPPLPGA
                     MSACVAGGAWEIDGVHFQFVWPPKGGHAAHDRKRVKRNDRACVLMVRGAHHSMLLTSD
                     VGLAQESALLHAGVQGVDVVMAAHHGSRHSSGEPFVRAMRARHVIAQAGKWNRYGHPD
                     QGVEARWKSSGATFWRTDRQGAVLVRSESTGLRVETTRSTQARYWQDKW"
     gene            complement(8547..9377)
                     /locus_tag="VFR20_13435"
     CDS             complement(8547..9377)
                     /locus_tag="VFR20_13435"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU0231564.1"
                     /translation="MLIDTHCHLDAGEFASDRDAVIARAQQTGVGVIVIPSVEHANFG
                     VVAALAQSFPGGAYALGIHPMYVPRARDEDLVYLEGCIQAALTDPRFVAIGEIGLDFF
                     VAELKTPEMAARQEHFYRVQLELAAHYGLPVLLHVRRSQDVLLKHLRQCQTVGGIAHA
                     FNGSFQQAHQFIERGFALGFGGAMTFPRARQIRRLAEQLPLEALVLETDAPDLAPAWL
                     GAGSSSARVRNEPAELAGISRELARLRGVDGAFLADMTARNARRALPRLKDAPGARPS
                     "
     gene            complement(9383..10090)
                     /gene="lolD"
                     /locus_tag="VFR20_13440"
     CDS             complement(9383..10090)
                     /gene="lolD"
                     /locus_tag="VFR20_13440"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEU0231565.1"
                     /translation="MTESTNVLQARSLHKVYDEGGERIEVLRDVSLHVARGEMVAIVG
                     ASGSGKSTLLHVLGLLDKPTSGSVLVEGSAVDGLSEARRSQLRNRALGFVYQFHHLLP
                     EFSSLDNVAMPLIVRRDKREAARREAEQVLEQVGLAHRMQHYPGQLSGGERQRVALAR
                     ALVTKPACVLADEPTGNLDRYTAEDMFDLLVRVNRDFGTAFAIVTHDEALAALAHRQL
                     HMHKGVLSDTAPVRQAG"
     gene            complement(10083..11357)
                     /locus_tag="VFR20_13445"
     CDS             complement(10083..11357)
                     /locus_tag="VFR20_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEU0231566.1"
                     /translation="MLYELWIGARYAGLARRRKKKGSRGDRFVSFIAASSMAGIALGV
                     AALIIVLSVMNGFQVQVRDRMLSVLPHIEMYVPGVAPQQVLTHWQALAADARKDSEVR
                     GEAPFVAAQAMVVRGQSLQGAQIRGIDPALETSVSDVGKQMVSGSIDSLKPGSYSIIL
                     GNQLAASLGVRKGDTVLVLAPQGSISPAGFAPRMRQFTVSGIFSSGYYEYDASLAFAN
                     YEDIGRVFRDSGVSGVRLRIADMQKAPEVARQLRRYVPDFVEIRDWTQSNRTWFAAVR
                     TEKRMMFLILALIVAVAAFNLLSSLVMAVKDKRSDIAILRTLGAGPKEIARIFLVQGA
                     IIGVMGTALGVFFGALIAYNIETIVPFFEHLFGVHFISQQIYFISELPSSPEVSDIVT
                     IAITSLILSLLATLYPSWRASKLQPAQVLRHD"
     gene            11400..12347
                     /locus_tag="VFR20_13450"
     CDS             11400..12347
                     /locus_tag="VFR20_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231567.1"
                     /translation="MQIVLPGALPDPGEARELAAHLPKAAPTLACWLGQSQARIFSTD
                     PAGVRCTPYEQWLLHTRGFTPRNDQPLCAGLGPMLANDGAPPDDEPIWLAELVHIAPS
                     RDGAALLPARDLAIEPEQSVALFDAAQTLLPGSGFDMRRLGVEHWRVLPGDAASLPIA
                     ASPTLVSLTSVNDWWPQDAATRPWRRLVNELQMLWFDHPVNQERTRLGLPEINSLWVF
                     GGASAKQFHSGRPAIESRTYPDLLEAHTRHDWSNWLAALGDLEARVFAPLANQRTTPD
                     LVLTGRDRYVELRSSALGKLRNWLPGGRDTWRNWWSPRH"
     gene            12329..14038
                     /gene="recJ"
                     /locus_tag="VFR20_13455"
     CDS             12329..14038
                     /gene="recJ"
                     /locus_tag="VFR20_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU0231568.1"
                     /translation="MVTPTLTDRPAHDGAARALESAGIHPLLARLWAARGIADATETR
                     LSWPDLIPPGSLTHCAQAASLLADAIATGKRLLIVADYDCDGATACAVGLRGLRGMGA
                     MVDFLVPNRFETGYGLSPAVVALAVRHPAGRPDILITVDNGIASVDGVDAARQAGMDV
                     IVTDHHLPGEHLPRALAIVNPNQPGCGFTSKNLAGVGVIFYLMLALRAELRRRGVYAP
                     DGGPRLDRLADLVALGTVADVVRLDANNRLLVTQGLGKMRNGHMQPGVRALFAVAGRE
                     PRQATAFDLGFALGPRINAAGRLSDMSLGINCLTTDDEGLALKLARELDTMNQERRAI
                     ESTMREEALAAMEGTQPEVGASVCVYHPDWHQGVVGLVASRLKETYWRPTLAFARSGE
                     GELRGSGRSIPDIHLRDALDLVSKRLPGAILKFGGHAMAAGLTLREEAYSDFVGGFDR
                     AVRDLSGRASFDPVVETDGSLESGYANAEVAELLQRQVWGAGFPAPIFRDTFRVGRQR
                     LLKDKHLKLTLERGHQHFDAIWFGHAEMLPDQVDVAYRLDRNEWNGIVSAQLVIEYAR
                     GVS"
     gene            complement(14076..16529)
                     /locus_tag="VFR20_13460"
     CDS             complement(14076..16529)
                     /locus_tag="VFR20_13460"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3772 domain-containing protein"
                     /protein_id="HEU0231569.1"
                     /translation="MNLMLLARRAPRSGQWAGRWRVGLVLLGMLVTLAMPALARGAGG
                     TTTLEVPKSVEVALDHARVEMDHVQNSLKGSLDDDQLVQLRLDVQKAQGQAEKAVTQL
                     DPELTSAKARLAELGPPPIGAPEAPDVAAQRTQLSRSSTKLDSQIKLARLITVEAGQA
                     VEQIQTLRRSQFQARLGERTDSVLGRRFWEGLQSEWPRDLRRVAPLSDEAVGLYQAVP
                     RLLLAGLSLAILAVLGLRVLTGRLLMRLTTTRVPSGRLRRSLFAVAQVLLAVVVPGII
                     AWLLCVGLSSPGDLSTTMNALLGRVVGLVCFGGAVAGLGRALLAPDRPTWRLPAIGDL
                     VARGLRWHPVVLAVIIVVGWLAQHLASDVNAGLSVIVAIDCADGLLVSLVLAHGMARA
                     GRLRRLARKQAERDSVSVNPLWLTALGGLAWLAIIVSVVCILLGYVAFGSFLVRQVIW
                     VATVLSATYLLTVLIDDGCGSLLAVIRRAANEAGGPRPLLSAHSQFLVMISGLSRVAI
                     ILFAVTLLLIPFGEGPSEWMNRFDQLYTGISIGEAQIRPSSVLLAVLVLILGFGGVRV
                     LQRWLLENYLPTTSLDLGMRTSASTLFGYAGYVLIVALSLSAAGIGLERVAWIASALS
                     VGIGFGLQAVVQNFVSGLILLAERPVKVGDWVSLSNVEGDIRRINVRATEIQMWDRST
                     VIVPNSEFITKIVRNVTHANPLGRVQIRLPIPVTTDAEQARSLMLDALASHEGVLDEP
                     APSVLLEGVDASGLLFVVTGYVGSPRVASGIRSDLLFDVLRRLREAGMALATPTTLLL
                     AEPPGSALPRIAAVAPPAR"
     gene            complement(16633..17625)
                     /locus_tag="VFR20_13465"
     CDS             complement(16633..17625)
                     /locus_tag="VFR20_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0231570.1"
                     /translation="MNPTQNPSRMLAYGCLALCMSLVGAYVALSKPLALAIPVFLLAW
                     MRFGIGGIAMLRWWRKPRAEPPLSPRIRLLVFLESFLGNFLFTIFMMYGVMMTGAVSA
                     GVIMSVIPAVVAVLGRVFLGERIGRRVLAAIACGVVGIGLLALTRHPGDAGTVSGHVW
                     LGDLFVFCSVLCEGFYAVIGKQLTSVLSPKRISAIINAWGFALMTPLGFYTALHFDFT
                     TVRPSIWLLLLFYSLAASVWTVWLWMTGLKTIPASRAGVFTVLLPVSAAIVGVLALGE
                     PFSGMHALSFAIALAGLVLATLPARGAASRPRGDTRFPMVSASAGARSVAHRNE"
     gene            17966..18798
                     /locus_tag="VFR20_13470"
                     /pseudo
     CDS             17966..18798
                     /locus_tag="VFR20_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
BASE COUNT         3668 a         6469 c         5796 g         2865 t
ORIGIN      
        1 acaaatcgcc gcgtcgcacg catccacggc cgctttccgc tcgacttgcc cagggaatcc
       61 ttgagccatt atagagaacc gcccgacgcg cgagcgaatg ccccatgccg caacggcgcc
      121 ccggggcacc aagcccgcac agccagaatc ctccaggcaa agccaggggg attctggcac
      181 ttggctaaat atgcagggcg tgccccagcg cgcgcagcgc cgcttcctgc accgcttcgc
      241 tcaacgaggg atgggcatga atggtgcccg ccacgtcctc cagtcgcgcg ttcatctcga
      301 tcgactgcga aaacgccgcg ctgagttccg acacgccgcc gcccacggcc tgccatccca
      361 ggatgagatg gttgtcggcg cgcgccacca cgcgcacgaa accctcggtg gactcgagcg
      421 tcatggcgcg gccattggcg gcgaacggga acgacgccgt cacgatgtcc aggcccgcct
      481 gcttcgcttc atcgggcccc agcccggccg ttgcgatttc cggatcggtg aagcacacgg
      541 ccggaatcga cgcaggagag aacgtacgcg gcttcccggc gatgatctcg gccacggttt
      601 cgccctgcgc catggcgcga tgcgccaaca tgggctcccc ggtcagatct ccaatggccc
      661 agacgttgtg catggaggta tggcagcgtt catccacctg gatcgcgcgc ccatccatat
      721 cgagcatgag cttttccaac ccccagcctt tgatgcgggg acgccggccc accgccacca
      781 ggaactgatc ggccgccagc tctgtttccg tcccatcggc ggcgcgcacg cgcaccgcat
      841 cacccgttgc cgtcaagccc tgcaccgaac agccaaggtg caagccgaca ccctgcttgc
      901 gcagggaagc cagaacgggt tgagtgagtt cggcgtcata ggcgggcaag atgcgatcga
      961 ccgcctcgac gacctggacg tcggccccga gcttgcggta gaccatgcca agctccaggc
     1021 cgatataacc cgcgcccacc acaaccagcc gatcaggaat gcgcgcgggc gacagcgcct
     1081 cggtggatga gatgacacgc cccccgaagg gcatgaaggg cagctccacg gcctcggacc
     1141 ccgccgccag cagcatgtgc cggcactgaa aagtctgaac accttccggc gcgtcggcat
     1201 cgaccgggcg aacgtcgacg gtcttgccat ccttgatttc cgcccagccg cgcacgaccc
     1261 gcaccccgtg cttcttcaac agaccggcca cacccgacgt gagccgacgc acgatgccat
     1321 ctttccaggc cacggtgcgg gtgacatcca acccggggct gcccacggtg atgcccaggg
     1381 ccgagccctt ggcgtaatgc gccacctttt cgaattcatg cgcggcatga atgagcgcct
     1441 tggacgggat gcagccgatg ttcaggcagg ttccgcccgg cgtcgcgccc tcgaccagca
     1501 ccgtgtcgat gccaagctgc gccgcgcgaa tcgcggccac atagccgccc ggcccgccgc
     1561 cgatgaccag cagcgtggtg gattgagcac tcatcgatct actccacgaa caaggttgcc
     1621 gggcattcca gatagccgcg cacgacctga atgaattccg cggcctgcat gccatcgacc
     1681 acgcggtggt cgaacgacga agacaggttc atcatcttgc gcgccacgac ggcgccaccg
     1741 aggatgaccg gacgttcgac gatgcggttg acccccacga tggccacttc gggatggttg
     1801 atgacaggcg tggacacgat accgcccagc gcgccgagac tggtgatggt gatggtggac
     1861 cccgacacct cgtcgcggct gaccttgccg gtgcgcgctt cgtcggccag acgggcgacc
     1921 tgccgcgcgc acgaccacag atcgtgcgtc tcggcatgac gcagtaccgg caccatcagg
     1981 ccggcctcgg tctgggcggc cacacccaga tgcaccggcc cgtattgcgt gaccacgccg
     2041 gcttcgtcat cgaaccgcgc gttgacctgc gggaaatcgc gcaccgccag caccacggcg
     2101 cggatcagga acggcagcag cgtcagtttg ccgcgctgcg cggcaaagcg tgcattgaga
     2161 cggacacgca atgtttccag ctcggtgacg tcgacttctt caacgtacga gaaatgcggg
     2221 atgcgacgct tggcttcctg cattttctgg gcgatgcggc gacgcaggcc aatgaccggc
     2281 acggcgtgct cgcccgtccg ttccgcatat ccagacgcgg gcgcgggcga caccacggtg
     2341 gcgccacgcg cggcataagt gtccaggtcg tcctgggtga tctgacccgc cttgccggtg
     2401 gccggcacga aacgcaggtc gatgccctgg tcggccgcgc gctggcgcac cgcaggcgaa
     2461 gccaggggtt tttctcccgg ctggcgcaac gcgccggacg gtgccatgga aagcggtttt
     2521 gcaacgctcg cctcggccgg cttcgaaaca gacgcgacaa cagaagccgc ggcagccggc
     2581 cgggctgcgg caccacttgt ccgaagcgtg gggatgcttg ccgaggacgc gggaacgctt
     2641 gccgtcgatg tcgacgtgct tatccgcgac gtgagggtgc ttgcttgtga tgaggcggga
     2701 accccacccg tcccgctggc gggagcgtct gactgcactg gtgaggggtt ggcgggcgtc
     2761 accgccccgc tcgtgtttgc cggcccgctg gccgcagcgg atgccgcctg gctgacaacg
     2821 tgcccatcac cggacgagcc ggcggtggtg gatgctggtg ccgtgcgacc cgcggtgcca
     2881 gtgggcgcat cgtgccgcga ttcgggtgca tggccagcgg ccgccgcgtt gcccgcgcct
     2941 tcgacttcta tgcgtatcag ttcggcgccc accgccatca catcgccggg ctggccgccc
     3001 agggacaaga ccttgcccac cactggggat ggcacctcga cggtggcctt gtcggtcatg
     3061 acgtcggcca gcaactggtc ttccgccacg tcgtcgccgg gctggacatg ccactcaacc
     3121 agttcgactt ccgcgatgcc ttcgccgata tccggcattt tgattatgtg tatacccatg
     3181 cttgttcctt tagccgcgaa ccaccgccca agcagcagtc cgcacacggc ggccacaggc
     3241 cgccgaccgg actctcgtca cccggcgtcc atgacgcggc gcagcgccgc acccacgcgc
     3301 gtgggatcgg gaaaatacgc ccattcgtgc gcgtgcgggt acggcgtgtc ccagccggtg
     3361 acacgctcga cgggtgcctc cagatggtag aagcaatgct cctgcaccag cgccacgagt
     3421 tcggcgccga acccgctggt gcgtgtggcc tcgtgcacca ccacgcaccg cccggttttc
     3481 ttgacagagt tgatgatggt gtcgaaatcg agcggccaga tgctgcgcag gtcgatgatc
     3541 tcggcgtcga tgccggtttc ctggaccgcg gcttccgaca cgaacaccat ggtgccgtag
     3601 gtgatgatgg tgaggtcatt acccgggcgc accaccgagg cggattccag cggcacggtg
     3661 tagtacccct cgggcacctg ccccttggga tgcttggacc agggcacgac cggccgatcg
     3721 tgatgcccgt cgaatgggcc gttgtacagc cgcttgggct ccaggaagat caccggatcg
     3781 tcgcactcga tggccgatat gagcagcccc ttggcatcgt acgggttgga cggaatgacc
     3841 gtgcgcagcc cgcaaacgtg tgtgaacagg gcctccgggc tttggctgtg tgtctgccca
     3901 ccgtagatgc cgccgccgca cggcatgcgt atggtcaggg gcgaaatgaa gtcacccgcg
     3961 gatcggtaac gcaagcgcgc ggcctcggaa acgatctgat cggaagccgg gtagaaataa
     4021 tcggcgaact ggatctcgac cacgggccgc aacccgtagg cgcccatgcc caccgccgcg
     4081 ccgacgatgc cgccctcgga aatcggcgca tcgaataccc ggtgcttgcc atatttggcc
     4141 tgcagccctt cggtgcagcg gaacaccccg ccgaaatacc cgacgtcttc gccgaacacc
     4201 accacgttct cgtcgctgcc cagcatgacg tccatggccg agcgcagcgc ctggatcatc
     4261 gtcatcgaga cgatgttgtc attcgtatcg ttcgccatga tcacaccccc agctgctgac
     4321 gttgccgacg caggtgctcg ggcatgtctt tatacacatc ttcaaacatg gacgacacac
     4381 tcgacatgtg gccgcccacc agcgttccgt ggccctcggc gactttctgc gccgcgatga
     4441 cctcggcctc gatctctttt tgcgcggcct catgctggtc gtcggaccag aggtccagct
     4501 tgatcatgtg ctgcttgagg cgggggatcg ggtcacccag cgggaagtgc gcccaatcgt
     4561 cggcaggacg gtacttggac ggatcgtcgg acgtggagtg cggccccgcg cgataagtga
     4621 cccattcgat gagggtgggc ccagcgttgc tgcgcgcccg ttcggcagcc cattgcgaca
     4681 cggcgtaggc cgcgaggaaa tcgttgccat ccacacgcag cgatgcgatg ttgttgccca
     4741 ggccgcgggc cgcgaacgtg gtgccctcgc cgccggcgat ggattggaac gtggaaatgg
     4801 cccactggtt gttgatgacg ttcaggatga ccggcgcgcg gtagacgtgg gcgaaagtga
     4861 gcgccgtctg gaagtcgccc tcggcggtgg cgccgtcacc gatccaggcc gatgcgatcc
     4921 tggtgtcgcc cttgatggcc gacgccatgg cccaccccac ggcctgcacg aactgcgtgg
     4981 ccagattgcc cgacaacgtg aagaaacccg catcggggta cgaatacagc accggcagtt
     5041 gacgcccctt gaggtggtcc aggtcgttgg agaacaactg gcacatcatg ttgaccagag
     5101 acatgtcctg cgcaatgagc aggctttgct gcctatacga cgggaaacac atgtcgcctt
     5161 tgcgcaaggc cagtatctgc gccgtgccga tggcttcctc gcccaggctt tgcatataga
     5221 acgacatttt cttctggcgc tgcgcgatca gcatgcgggc gtcgaacacc cgtgtcttga
     5281 tcatggagcg catcgcatgc cgcagcatgg ccgggtcgat gtccggtgcc cagggaccga
     5341 ccgcgttgcc gtcgtcatcg agcacacgca ccagggaata ggccaggtcg gaaatatcga
     5401 gaggggaaac gtcgacggga ggtcggcgtg ccgcgccggc gggcgcgagt tggagaaagg
     5461 aaaagtcagt tggctgaccg ggacgcccgg agggctccgg tacgtgcaac cgtagcggaa
     5521 tgtcttgtgt catggctgtt ttggtcctcg atgccgaacg cgtcttgtgg acagattcgt
     5581 cgtcgaaatg atcttgtgga tacttgttcc atgatcgccg attcgggcca aatccgcaag
     5641 gttggcccca atgggtcggg ccggcggtct cgcccggatt ttcacctatt gccagggcca
     5701 tcatgagcca aaaatattgc caggcaacaa tattcgtgca gaaaatcaga tagttaccag
     5761 tttcgtccgg ggggacaggg ccagaaagcc gcgggcagcc cgatctcatc ccgacacggc
     5821 aacaccctcg ttcaccactt atcctgccag tatctcgcct gcgtcgaccg ggtggtttcc
     5881 acgcgcaggc ccgtggactc cgaacgcact aagacggccc cttgtcgatc cgtgcgccag
     5941 aacgtcgcac cgctcgattt ccagcgcgcc tccactccct gatcgggatg cccgtatcga
     6001 ttccatttgc cggcctgagc gatcacgtgc cgcgcccgca tggcccgcac aaacggctcg
     6061 ccggacgagt ggcgcgaccc atggtgcgcg gccattacca cgtcaacccc ctgcacaccg
     6121 gcatgcaaca acgccgattc ctgtgcaagg cccacatcgc tggtgagcag catcgagtga
     6181 tgcgcgcccc gcaccatcag cacacaggcc ctatcgttgc gcttgacgcg cttgcggtca
     6241 tgcgcggcat gaccgccttt cggtggccag acgaactgga aatggacacc gtcgatttcc
     6301 caggcaccac ccgcgacaca cgccgacatg gcgccgggca gcggcgggag atcgtccggc
     6361 cgtccgagca gacgggcctc acgacgcaga tacgctgcca gatcgaacga actgtacgat
     6421 tgcatcaccg gcagcgccga aagcaggctg cgcgcaccac ccgcatgatc gatgtcggca
     6481 tgggacacca ccaacgcatc gatgcggcgc acgccggtgg cccgcaaata cggcagaatc
     6541 gtgcgggcgc ccgagtccga atcgggtccg gtgcgcagac cggtatcgaa caccagcgcg
     6601 tgacgggccg tctgaacgac gaccgcgccg gcttgaccga catccagcgc ggccagacgc
     6661 cactccccct ggccgggccg atccgcacgc caccatacgg cggggatcag tagcagccag
     6721 gcgatacgcc cgtcgcggaa gccccacgac aggatggcga tcaccagccc cgccgaagcg
     6781 gacaccaaca cccacaacgg gggtgcggcg gcgtcaaacg tggcgaccgg gctcccggcc
     6841 agccacaccg tcggcgccat gacgcttgca agcacgccat gacccagcca cgcgaacgct
     6901 ccggccagcc aatccaaacc cggcaccagc gcggctgcgg cggtcagcag ggacagcggc
     6961 gtgacaagca attcgacggc gggaatggca tacgcgttga ccagcggcga gatcaccgac
     7021 acctgatgaa agacaccggc cagaatcggt gtcagcccgg ccgtgatcgc cagttgcaag
     7081 cgcgtcgcca acccaacgga ttggcggaaa cgatcccagc gtgaaggcgt cgagcgggtc
     7141 atcggatgtc cgctccagcc gccacttgcc aacaatatgc ccaccgcgcc gaaggacagc
     7201 caaaagccac tggccagcac cgcccagggg tcgagagcga ccacgacgaa caggaccaga
     7261 cacagcgcgc gcgacacacc aagcgggatt ctcgcggcgc gggcgcaagc cagcaccgcc
     7321 agcatgacga aggtgcgccg ggccggcaca ccccaaccgg ccagcaggca atacacccac
     7381 gccaccagca acgccgccat cgcgcccgcc acctgcgcgg gcacacgctc ggccagcgcg
     7441 cgtccaccca aacgcgtcaa tggccaaaga cgcaacgcaa ggacgccgcc cagcgcggcg
     7501 atcatggtga tatgagatcc gctgatggaa accaggtgcg taatgccggt gacgttaaag
     7561 attgtccagt cgccgctcgg cacactatcc tggtcaccaa tcgccagggc gagctgcacg
     7621 gcaccatagc gacggccctc cagatacggc cgcatcgcct tacgcaccac atggcgggcg
     7681 cgctcggcca ctaccggcag gctcgcccat ggctcgtcac gcagatatcg cggcgtcccg
     7741 cgcacgctgc ccgtcgcacg gatcccacgc gcgaacagat gaccctcgta gtcgaaacca
     7801 tggggattgc gctcaccgtg tggaaccttc agcgtgagcg ccatgcgcca gatctggcca
     7861 ggtatgagat cgggaaagtc ggccggccgg ctgtcgccgc tgccataagg cccgccccat
     7921 tttcccaacg accacgacac cagaatgcgc gaaggcacac ccatgggcct ggaagacagg
     7981 acctcggcct cgaactgtcg gcggtccggg gaaagtctgg gcagcgtggc gatgcgcagc
     8041 acgacacgcg acaccttgtt ttcattatca ggcgccaggc ggtcgtccag aagggcctgg
     8101 gcgcgccaga cagtcgaggc gaacgcgata tatgccaccc aaagcacgag catgatggtc
     8161 gtcgccaccc ggcaacgtga cgcggctccg ccggacagcc ggatcggaac cgaccaaccg
     8221 tggcggcaca agcaccgggc ggccattgcg acgcacgaca caatgaccag ggcaatgccc
     8281 gtccacacca gggacacccg ccccggaagc acggacaggc tgtgcacggc tcccgaagcc
     8341 gccacggctg tcaacaggca gaaacgtccc ctcacgtgcg atcatcccca aatgatccat
     8401 gttagggatg ggagccgcgc ctgggcggcg aggtttgatc aaaatgtccc gtcgattaat
     8461 acgaacgcga cagacagtcc gaagccacag aggcatgcgg caaggcagcc tgatcgttag
     8521 aggggcctgg attcagacat tcaaaatcag cttggtcgcg caccaggcgc atctttcagc
     8581 cggggcagcg cccgccgcgc attgcgcgcg gtcatatcgg ccagaaacgc gccgtcgaca
     8641 ccgcgcaatc ttgccagttc ccggcttatg ccggccagtt cggccggctc gttgcgcacc
     8701 cgcgcggatg acgagcccgc gcccagccac gccggcgcca gatccggcgc atcggtttcc
     8761 agcaccaggg cctccagcgg caattgctcg gcgagccgcc gtatttgccg tgcgcgcgga
     8821 aatgtcatcg ccccgccaaa ccccagcgcg aagccgcgct cgatgaattg atgcgcctgt
     8881 tgaaagctgc cgttgaacgc atgcgcgata ccgccgaccg tctggcattg acgcaaatgc
     8941 ttcagcagca catcctgcga gcgtcgcaca tgcaacagca caggcaaacc gtaatgcgcc
     9001 gcgagctcga gctggacacg atagaaatgt tcctgccgcg ccgccatctc cggcgtcttc
     9061 aattccgcca cgaagaaatc caggccgatc tcgccaatgg cgacgaaacg cgggtccgtg
     9121 agcgcggcct gtatgcagcc ttcgagataa accagatctt catcccgcgc gcgcggcacg
     9181 tacatgggat ggatgcccaa cgcataggcg ccgcctggaa atgactgggc caacgcggcc
     9241 accacgccga aattggcgtg ctcgacggat gggatcacga tcacgcccac accagtctgt
     9301 tgcgcacgcg cgatgaccgc gtcgcgatcc gacgcgaatt cgcccgcgtc caggtggcaa
     9361 tgcgtgtcga tcagcacggg cgtcacccgg cctgtctgac cggggccgta tcggacagca
     9421 ctcccttgtg catgtgaagc tggcgatgcg ccagcgccgc cagcgcctcg tcatgggtga
     9481 cgatggcgaa ggcggtgccg aaatcgcgat tcacacgcac cagcaaatcg aacatgtctt
     9541 cggccgtgta gcggtcgagg ttgccggtgg gttcatcggc cagcacacaa gccggcttgg
     9601 tcaccagggc gcgcgccagc gccacgcgtt gacgctcgcc gccggacagc tgaccggggt
     9661 agtgctgcat gcgatgcgcc aggccgacct gttccagcac ctgttcggct tcgcggcgcg
     9721 ccgcctcgcg tttgtcgcgc cgcacgatca acggcatggc gacgttgtcc aatgacgaaa
     9781 actcaggcag caaatgatgg aactgataga caaaccccag cgcgcggttg cgcaactggc
     9841 tgcggcgcgc ttccgacagg ccatcgaccg ccgaaccctc gaccagaacc gacccggacg
     9901 tgggtttatc cagcagcccc agcacgtgca gcaaggtgct cttgcccgaa ccggacgcac
     9961 cgacaatggc caccatctcg ccacgtgcga catgcaggct gacatcgcgc agcacctcga
    10021 tgcgttcgcc gccttcgtca tagaccttgt gcaggctgcg tgcctgcaga acgttcgtgg
    10081 attcagtcat ggcgcagcac ctgtgcgggc tgaagtttcg atgcgcgcca gctgggatac
    10141 agcgtggcca ggagagaaag gatcaaggat gtgatcgcga tggtcacgat gtcggacacc
    10201 tcggggctgg agggcagctc actgatgaag tagatttgct gggagatgaa atgcacgccg
    10261 aacagatgtt cgaagaacgg cacgatggtt tcgatgttgt aggcgatcag cgcgccgaaa
    10321 aacaccccca gcgccgtccc catcacccca atgatcgcgc cctgcacgag gaatatgcgg
    10381 gcgatttcct tcgggccggc gcccagtgtg cgcagaatgg cgatgtcgga gcgcttgtcc
    10441 ttcacggcca tcaccagcga agacagcagg ttgaacgccg ccaccgccac gatcaatgcc
    10501 aggatcagga acatcatgcg tttctctgtg cgcaccgcgg caaaccaggt ccggttgctt
    10561 tgggtccagt cgcggatctc gacgaaatcc ggcacatacc gccgcagttg gcgggcgact
    10621 tccggcgcct tctgcatgtc ggcgatgcgc agccgcacgc cgctcacgcc gctgtcgcgg
    10681 aacacgcgac cgatgtcctc atagttcgcg aaggccagcg aggcgtcgta ttcgtaatag
    10741 ccggaagaga aaatccccga cacggtgaac tgccgcatgc gcggcgcgaa gccggcgggt
    10801 gagatcgaac cctgcggcgc cagcaccagc acagtgtcac ccttgcgcac acccaacgac
    10861 gccgccagct ggttgcccag gatgatggaa taactgcccg gcttgaggga atcgatgctg
    10921 cccgacacca tttgtttgcc cacgtcggaa acggacgtct caagcgccgg gtcgatgccc
    10981 cggatctgcg caccctgcag cgactgcccg cgcacgacca tggcctgggc ggcgacgaat
    11041 ggcgcctcgc cacgcacctc gctgtccttg cgggcatcgg ccgccagcgc ttgccagtgt
    11101 gtcagcacct gttgcggcgc cacgccgggc acgtacattt caatgtgcgg cagcaccgac
    11161 agcatgcggt cccggacctg cacctggaaa ccattcatca ccgacagcac gatgatgagc
    11221 gcggccacac ccagggcgat gccggccatc gagctcgcgg cgatgaacga cacgaagcga
    11281 tcgccccggc tgcccttttt cttccgccga cgcgccagtc cggcataccg cgcccctatc
    11341 cacagttcat acagcatggt cgatcagcca ccgtctttcg ttgacactac aatggaaaaa
    11401 tgcaaatcgt actccctggc gcactgcccg accccggcga ggcgcgcgaa ctcgccgcgc
    11461 acctgcccaa agccgccccc accttggcgt gctggttggg ccaaagccag gcccggatct
    11521 tttcaaccga tccggctggg gtacgctgca ccccctacga acagtggttg cttcacacgc
    11581 gggggttcac cccacgcaac gaccaaccgc tttgcgccgg tctgggtccg atgctggcca
    11641 atgacggcgc cccgcccgac gacgagccca tatggctggc cgaactcgtg cacatcgcgc
    11701 cctcgcgcga tggcgcggca ttgctgcccg cgcgcgacct ggccatcgag ccagagcaga
    11761 gtgtagcctt gttcgacgcc gcgcagaccc tgctgccggg ttccggcttc gacatgcggc
    11821 ggctgggcgt cgaacattgg cgcgtgctgc ccggcgatgc ggccagcctg cccatcgccg
    11881 ccagccccac gctcgtcagc ctgacgtcgg tcaacgactg gtggccacaa gacgcggcca
    11941 cccggccctg gcgccgcctg gtcaatgaat tgcaaatgct ctggttcgat cacccggtca
    12001 accaggaacg aacccggctc ggcctacccg agatcaacag cctatgggtc tttggcggcg
    12061 caagcgccaa acagttccat tccggcaggc ccgcgatcga atcgcgaacc tatccggact
    12121 tgttggaagc acatacccga cacgactgga gcaactggct ggcggcgctg ggcgacctgg
    12181 aagcccgcgt cttcgccccc ctggcgaacc agcgaacgac gcccgacctg gtcctgacgg
    12241 gacgcgatcg ctatgtcgag ctgcgatcga gcgcattggg caaactacga aactggctgc
    12301 cgggcggccg cgacacatgg agaaactggt ggtcaccccg acactgacag accgaccggc
    12361 gcacgacggc gccgcgcggg cgctggaatc cgcgggcata cacccgctcc tggcacggtt
    12421 gtgggcggcc cgtggcatcg ccgacgccac ggagacacgc ctgagctggc ccgacctgat
    12481 cccacccggc agcctcacgc attgcgccca ggccgccagt ctgctggccg atgccatcgc
    12541 cacgggaaaa cgcctgttga tcgtggccga ctacgactgt gacggcgcca cggcttgcgc
    12601 ggtgggcctg cgtggcttgc gcggcatggg cgccatggtc gatttcctgg tccccaaccg
    12661 cttcgaaacc ggatacgggc tctcgcccgc cgtggtggcg ctggcggtgc ggcacccggc
    12721 cgggcgaccc gacatcctca tcaccgttga caacggcatc gccagcgtcg acggtgtcga
    12781 cgcggcgcgc caggccggca tggacgtgat cgtcaccgac catcacctgc ctggcgagca
    12841 ccttccccgc gccctggcga tcgtcaaccc aaatcaaccc ggttgcggct tcacatcgaa
    12901 gaacctggcg ggagtgggcg tcattttcta cctgatgctc gccttgcgcg cagagcttcg
    12961 gcggcgtggc gtgtacgcac cggacggtgg gccgcgcctg gatcgtctgg ccgacctggt
    13021 cgcgctgggc accgtcgccg acgtggtccg tctggacgcc aacaaccggc tcctggtcac
    13081 ccagggcctc ggcaaaatgc gcaacggcca tatgcagcca ggcgtgcgcg cgctgttcgc
    13141 cgtggcgggg cgcgaacccc gccaggccac tgcgttcgat ctgggttttg ccctggggcc
    13201 gcgcatcaac gccgctggcc gcctgtcgga catgagcctg ggcatcaact gcctgacgac
    13261 cgatgacgag ggtctggcgc tgaagctggc gcgcgaactg gacaccatga atcaagagcg
    13321 gcgcgccatc gagtcgacga tgcgcgaaga agcactcgca gccatggaag gcacgcagcc
    13381 cgaggtcggc gcatcggtct gcgtctacca tccggactgg catcaggggg tggtcggcct
    13441 ggttgcctcc cggctcaagg aaacctactg gcgccccacc ctggcgttcg cccgcagcgg
    13501 cgagggcgag ttgcgcggct cgggccgctc catccccgac atccacctac gggacgcgct
    13561 ggacttggtc tcgaagcgcc tgcccggcgc catcctgaaa ttcggcggcc atgccatggc
    13621 ggccggcctg acgctcaggg aagaagccta cagcgatttc gtaggcgggt tcgatcgagc
    13681 cgtgcgggat ctgagcggac gcgcctcgtt cgatccggtc gtcgaaaccg atgggtcgct
    13741 ggaatccggc tacgccaacg ccgaagtcgc ggaactgctg caacgccaag tgtggggggc
    13801 cggcttcccc gcccccatat tccgtgacac attccgcgtt ggccggcagc gcctgctgaa
    13861 ggacaagcac ctgaaactca cattggaacg tggccaccag catttcgacg ccatctggtt
    13921 cgggcacgcc gagatgctgc ccgatcaggt ggacgtcgcc taccggctgg accgcaatga
    13981 atggaacggc atcgtcagcg cccaattggt catcgagtac gcgcgcggtg tttcatgaca
    14041 gggccggcaa gcatgcccgc gcgatgcgat ttcagtcagc gagccggggg cgccaccgcc
    14101 gcgatccgcg gcagcgctga tcctggaggc tcggcaagca gcagcgtggt cggggtcgcc
    14161 aacgccatcc cggcctcccg cagacgcctg agcacatcga acaacaaatc gctgcggatg
    14221 ccggaagcca cgcggggcga acccacatag cccgtgacga cgaacaacag accgcttgca
    14281 tccacgccct cgagcagcac actgggtgcc ggttcatcca gcactccctc atggctcgcc
    14341 agggcgtcaa gcatcaggct gcgcgcctgc tcggcatcgg tggtgaccgg tatgggcagc
    14401 cggatctgca cccggcccag cggattggcg tgcgtcacgt tgcgcacgat cttggtgatg
    14461 aactcggaat tcggcacgat caccgtggaa cggtcccaca tctggatctc ggtggcgcgc
    14521 acgttgatgc ggcggatatc gccctcgacg ttgctcaggg acacccagtc gcccaccttg
    14581 accggccgct cagcaaggag aatcagcccg gatacgaaat tctgcaccac cgcctgcagc
    14641 ccgaagccga tgccgaccga cagggcgctg gcgatccagg ccacccgctc gagcccgata
    14701 ccggccgccg acaaggagag cgccacgatg agcacataac cggcgtaccc gaacagcgtg
    14761 gacgccgaag tccgcatgcc aagatccaga ctcgtggtgg gcaggtaatt ttccagcagc
    14821 caacgctgga gaaccctcac gccgccaaag ccaaggatca gcaccaacac ggccagcagc
    14881 accgatgacg gacggatttg cgcctcaccg atggaaatgc cggtgtacag ttgatcgaat
    14941 cggttcatcc attcggatgg cccttcaccg aaggggatca acaaaagtgt caccgcgaac
    15001 aggatgatgg ccaccctgct caagccggaa atcatcacca ggaactggct atgcgcgctc
    15061 aacaaggggc gtgggccacc cgcttcgttc gccgcccgcc ggatgacagc caacaagctg
    15121 ccgcagccgt catcgatgag cacagtcagc aaatacgtgg cgctcaacac cgtggcgacc
    15181 cagattacct gccgcaccag gaaactgccg aatgcaacgt accccaacag aatgcagacc
    15241 acgctgacga tgatcgccag ccaggccagg ccacccagcg cggtcagcca cagcgggttg
    15301 acagacaccg aatccctttc agcctgtttc cgggccagac gacgcaagcg gcccgcacgc
    15361 gccatgccgt gcgccagaac caggcttacc agcaagccat ccgcgcagtc gatggcgacg
    15421 atgacgctca ggcccgcatt gacatccgag gccaggtgct gcgccagcca gcccactacg
    15481 atgatcactg ccaggaccac gggatgccag cgcaaacccc gcgccacgag gtctccgatg
    15541 gcgggtaacc gccatgtggg gcgatcaggc gccagcagag cgcgccccag acccgccacg
    15601 gcgccaccaa agcacaccag ccccaccact ctgcccaaca gagcgttcat ggtggtggac
    15661 aaatccccgg gcgagctcaa gccgacgcac aacagccacg cgatgatacc cggcaccacg
    15721 actgccaata acacctgcgc cacggcgaac agcgaacgtc gcaaccgccc cgaaggcacc
    15781 cgcgtggtgg tgagccgcat gagcaggcgc cccgtcagca cacgcaaacc cagcacagcc
    15841 agaatggcca gggacaaccc ggccagcaac agccgcggca ccgcctggta caaccccact
    15901 gcctcatcgg acaaaggcgc cacacgacgt agatcgcgcg gccattccga ttgcagccct
    15961 tcccagaaac ggcgccccag aacggaatcc gtgcgctctc ccaggcgcgc ctggaactgc
    16021 gagcgacgca gggtctggat ttgttcgaca gcctggccgg cctcaacggt gatcagccgc
    16081 gccagcttga tttgcgagtc gagcttggta ctggaacggc tgagctgggt gcgctgcgcc
    16141 gcgacgtccg gcgcctcggg cgccccgatc ggcggcggcc ccagttcggc caatcgggcc
    16201 ttggcgctgg tcagttcggg atcgagctgc gtgacggcct tctcggcctg cccctgcgcc
    16261 ttctggacat cgagacgcag ttgcactagc tgatcatcgt ccagcgagcc cttgaggctg
    16321 ttctgaacat gatccatctc gacccgggcg tggtccagcg ccacctcgac actcttcggg
    16381 acttccagcg tcgtcgtgcc gccggcgccg cgtgccagcg ccggcatggc gagagtcacc
    16441 aacatgccaa gcaagacaag acccacccgc cagcggccgg cccattgccc ggagcgcggc
    16501 gcccgtcggg caagcagcat cagattcatt ttcgtgcttt cacgcgttga ggtcgcctat
    16561 tttatgctgt ggggctgacg acgcgcacgg cctgaatgga actgaaacaa caacgttaca
    16621 agacgtgctc gattactcat tccgatgagc aaccgagcgg gcgcccgcgc tcgccgacac
    16681 catcgggaag cgcgtgtcgc cccggggccg gcttgcggcg ccccttgcgg gcaaggtagc
    16741 cagcaccagc cccgcgaggg cgatggcgaa cgacagcgca tgcatgccgg aaaaaggctc
    16801 acccagcgcc aggaccccaa cgatcgccgc gctgacgggc agcaagacgg tgaacacccc
    16861 ggcacgagac gccggaatgg tcttcagacc cgtcatccag agccacaccg tccatacgct
    16921 ggcagccagg ctgtaaaaaa gcagcagcaa ccaaatggat ggccgcacgg tggtgaagtc
    16981 gaaatgcaac gcggtgtaaa accccagcgg agtcatcagc gcgaaccccc aggcgttgat
    17041 gatggcgctg atgcgcttcg ggctgagcac gctggtgagt tgcttgccga tgacggcgta
    17101 gaacccctcg cacagcacgg aacagaagac aaacagatca cccagccaga catggccgga
    17161 gaccgtgccc gcatcgccgg gatgccgggt caacgccagc aggccaatgc cgacaacacc
    17221 gcaggcgatg gccgccagca cacggcggcc gatccgttcc cccaggaata cgcgccccag
    17281 caccgccacc actgcgggga taaccgacat gatgacgccc gccgacaccg caccggtcat
    17341 catgacgccg tacatcatga agatggtgaa caggaaattc cccaggaacg actccaggaa
    17401 caccaggagc cggatgcgcg gcgacagggg tggctcggcc cggggtttgc gccaccaccg
    17461 cagcatggcg atgccgccta tgccgaaacg catccacgcc agcaggaaca ccgggatcgc
    17521 cagcgccagc ggtttgctga gcgccacgta ggcccccacc agcgacatgc agagcgccag
    17581 gcagccatag gccagcatgc ggctcggatt ctgggtggga ttcatggaga cggcatattc
    17641 gaaaggcgcc acacgcccgg tccgaagcca caaaagcggg cctgccggaa gcaagcatcc
    17701 aacgatagca caggcggaac atggggattg tggcgacgcc ctgcctgagc cccggggaaa
    17761 tgccccttcg cgaccctccc caagcccccc cgtggacgca tcgggttaaa atccgatgat
    17821 caacgagcac gaggcccaca gcgttattcc cgaaacaccg cattcgtggg ccacgccctt
    17881 gacgtcgcct gatgcggaac gacccggaat catcgcgaca actctgcccg aatccaccat
    17941 ccaccccctc attaccggac acatcatgga agccgaacgt caaaatcaac tcgccgcccg
    18001 cttggccgac tacgcacaac gcgagtccga cctacggggg tatctttgac tacgatgcca
    18061 agtccgaacg cctggccgtc gtcaatgccg aactcgaatc ccctgacgtc tggaacgacc
    18121 ccaagcacgc ccaggaactg gggcgcgaaa agaagagcct cgaacacatc gtcctgaccc
    18181 tgtccgcgct gggaaagggg ctggctgacg ccgccgagct gttcgagctg gccagcgccg
    18241 acgaggacga cgccacgctg cacgccatcg aagccgactg cgacgccatg ggcgggcaga
    18301 tcgaggcgct ggagttccgc cgcatgttct cgaatccggc cgatccgctg aactgcttcc
    18361 tcgacatcca gtccggcgcc ggcggcacgg aagcccagga ctgggcctcg atgctgttgc
    18421 gccagtacct gcattactgc gagcgcaagg ggttcaaggc cgaggtcctg gaagaatccg
    18481 agggtgaagt ggcgggcatc aaatcggcca ccatcaagat cgagggcgac tacgccttcg
    18541 gctacctgcg caccgaatcc ggggtgcacc gcctcgtgcg caaaagcccg ttcgattccg
    18601 ccaacggccg ccacacgtcc ttcgccagcg tcttcgtcta ccccgaagtg gacgactcca
    18661 tcgagatcga catcaacccg agcgacgtgc gcaccgacac cttccgggcc agcggcgcgg
    18721 gcggccagca catcaacaag acggactccg ccgtacgcat cacgcacatg cccagcggca
    18781 tcgtcgtgca gtgccaga
//
LOCUS       DASTNK010000184         3808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_547784, whole genome shotgun sequence.
ACCESSION   DASTNK010000184 DASTNK010000000
VERSION     DASTNK010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547784"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="VFR20_13475"
     CDS             complement(<1..140)
                     /locus_tag="VFR20_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU0231571.1"
                     /translation="MPKATPDSRPTLLQGLITAAHGRHYMVELADGTLRTCFPRGKKA
                     GAA"
     gene            complement(133..1377)
                     /locus_tag="VFR20_13480"
     CDS             complement(133..1377)
                     /locus_tag="VFR20_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU0231572.1"
                     /translation="MFTLLFIVFLFAGFIVRLWLATRQVRHVRGHRDAVPPEFADRIS
                     LHSHQRAADYTLARIRVNLVEQCVDTLVLVALTLLGGLQFLDKHLGLLIGSEMPRQLA
                     LIASVLVALGVIELPFSAWRKFRLEARFGFNRVTPRLFVADTLKGLALGIVLGLPLVA
                     AVLWVMANAGTAWTWWAWAVWVAFNILILWAFPTLIAPLFNKFTPLDDPALADRIRAL
                     MTRCGFSLGGLFVMDGSKRSAHGNAYFTGFGKARRIVFFDTLLNRLDGDEIEAVLAHE
                     LGHFKHRHIIRRMILGFILALVFFLVLGWASQQVWFYTGLGVLPQLGRPNDALALILF
                     FLAVPVFTFWVAPLGNWLSRRDEFQADQYSASQCSPEALASALVKLYDDNAATLTPDP
                     VHSAFYDSHPPATVRIHHLRHA"
     gene            1415..1960
                     /gene="orn"
                     /locus_tag="VFR20_13485"
     CDS             1415..1960
                     /gene="orn"
                     /locus_tag="VFR20_13485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEU0231573.1"
                     /translation="MAVNDQRLVWLDMEMTGLDPEKERIIEVAVVVTEPDLTLVAEGP
                     VLVVHQSDELLDGMDKWNKSTHGKSGLIDKVKASTLTETQAEDQLLAFLGQHLPAGKS
                     PLCGNTISQDRRFMVKYMPRLEAFFHYRNLDVSTLKELALRWKPEVYKSFVKRSRHEA
                     LADIYESIDELKHYREHFIKL"
     gene            complement(1955..2938)
                     /gene="paaX"
                     /locus_tag="VFR20_13490"
     CDS             complement(1955..2938)
                     /gene="paaX"
                     /locus_tag="VFR20_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HEU0231574.1"
                     /translation="MDTLSRTPNSLVSALLEQHPPRAKSLCVTLLGDAIGPHGGQVWL
                     SDLIELVTPLGINERLLRTSVFRLVAQGWLRSERHGRRSLYRLSPQGEALTREASARI
                     YEGAATDWEGDWTLVIVPRFGNGSLDQRGQLRRELIWAGFGAIAPGVFTLPRRQTERA
                     RKVLGNLKATDKALVLSAHDLDDGHGLALQALITQCWNLEEIARQYRAFSEAFSPLLA
                     QGDWGAAPEQAFAARALALHEWRRIVLQDPQLPHQMLPVDWPGHAARALCGNLYWRVF
                     DPAELFLEPILRRNPEQFEALPRHVRQRFRANVVAGTPDGEPVSDGDTDAL"
     gene            3107..>3808
                     /gene="paaA"
                     /locus_tag="VFR20_13495"
     CDS             3107..>3808
                     /gene="paaA"
                     /locus_tag="VFR20_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEU0231575.1"
                     /translation="MYAQLVDTGVAKVRDAATLSGPEKAFQQRVDDGIRIEPKDWMPS
                     AYRKTLVRQISQHAHSEIVGMLPEGNWITRAPSLKRKAILLAKVQDEAGHGLYLYSAA
                     ETLGVSRDDLVDDLLTGKAKYSTIFNYPTLTWADIGMIGWLVDGSAIINQIPLCRCSY
                     GPYARAMVRVCKEESFHQRQGYDLLIQMCRHGTAAQKDMAQDALNRWWWPALMMFGPP
                     DAESVNSAQSMQWRIK"
BASE COUNT          786 a         1121 c         1242 g          659 t
ORIGIN      
        1 gcggcccccg ccttcttgcc ccgggggaag caggtgcgca gggttccgtc ggcgagttcg
       61 accatgtaat ggcgcccgtg ggcggccgtg atcaggcctt gcaataacgt gggacgggag
      121 tccggcgttg ccttaggcat gtctgaggtg gtggatgcgc acggtggcgg gagggtggct
      181 gtcgtagaag gcggaatgga caggatcggg ggtcagcgtg gccgcgttgt catcgtacag
      241 cttgaccagt gcggatgcca gggcctcggg tgaacactgg cttgcggagt attggtccgc
      301 ctggaattcg tcccggcgcg agagccagtt gcccaggggc gcgacccaga acgtgaagac
      361 gggaaccgcc aggaagaaaa gaatcagagc cagggcgtca ttggggcgtc cgagctgggg
      421 cagcacaccc agaccggtgt agaaccagac ctgttgggat gcccagccca gcaccaggaa
      481 gaacaccagc gccaggatga agccgaggat catgcggcgg atgatgtgcc ggtgtttgaa
      541 gtggccaagt tcgtgcgcga gcacggcctc gatttcgtct ccgtccaggc gattgagcag
      601 cgtgtcgaag aaaacgatgc gccgtgcctt gccgaagccg gtgaaatagg cattgccatg
      661 cgccgaacgc ttcgatccgt ccatgacgaa aagcccgccc aatgagaaac cgcagcgggt
      721 catgagtgcc cggatgcgat ccgcgagggc cggatcgtcg agtggtgtga acttgttgaa
      781 caaaggggcg atgagggtgg ggaaggccca taggatcagg atgttgaagg ccacccagac
      841 cgcccatgcc caccacgtcc aggctgtgcc ggcgttggcc atgacccaca gaacggcggc
      901 cacaagtggc aggccaagca cgatgcccag ggccaggccc ttgagcgtgt ccgcgacgaa
      961 caggcgtggc gtgacgcggt tgaatccgaa ccgcgcctcg aggcggaact tgcgccaggc
     1021 ggagaacggc agctcgatga cgcccagcgc caccagcacc gatgcgatca gcgccagttg
     1081 gcgcggcatt tcgcttccga tcaacaaacc caggtgtttg tccaggaact gcaacccgcc
     1141 gagcagcgtg agggcgacga gcaccagagt gtccacgcac tgttcgacca gattcacgcg
     1201 aatccgggcg agtgtgtagt cggccgcgcg ttggtggctg tgcaggctga tgcggtcggc
     1261 gaattccggt ggcaccgcgt cgcggtgccc gcgaacgtgg cgaacctggc gtgtggcgag
     1321 ccacaggcgc acgatgaatc cggcgaacag aaagacgatg aacagcagtg tgaacatggg
     1381 ttgtgcgaaa atccttcttt taaggacgga ttatatggcg gttaatgatc aacgcttggt
     1441 ctggctggac atggaaatga ctggcctgga cccggaaaag gaacgcatca tcgaagtggc
     1501 cgtggtcgtg acggaacccg acctgaccct ggtggcagaa gggccggtgc tggtcgtgca
     1561 ccagagcgac gaactgctcg atggcatgga caaatggaac aagtccacgc atggcaagag
     1621 tggtctgatc gacaaggtca aggcatccac gctgactgaa acccaggccg aagaccagtt
     1681 gctggccttt ctggggcaac atctgccagc agggaaatcg cccctgtgcg gcaacaccat
     1741 cagccaggat cgccgcttca tggtgaaata catgcccagg ctggaagcat tcttccatta
     1801 ccgcaatctc gacgtcagca cgttgaagga actggcgctg cgttggaagc ctgaagtcta
     1861 caagagtttc gtcaagagaa gtcgccacga ggcgctggcc gacatctatg aatccatcga
     1921 cgaactcaag cattatcgcg aacacttcat caagttatag cgcgtcggta tcgccatcgc
     1981 ttaccggctc gccgtctggc gtgccggcca ccacgttggc gcgaaagcgc tgacgcacgt
     2041 gccgaggcag tgcctcgaat tgttcggggt tgcgacgaag gatgggttcc aggaacagct
     2101 cggcgggatc gaagacgcgc caatacaggt tgccgcacaa cgcccttgcc gcatggccgg
     2161 gccaatcgac gggcagcatc tggtgcggca gttgcgggtc ctgcagaacg attcggcgcc
     2221 attcgtgcag tgccagggcg cgcgccgcga acgcctgctc cggtgccgcg ccccagtcgc
     2281 cctgtgccag caagggcgag aaggcttctg aaaacgcccg gtattgccgg gcgatttcct
     2341 ccaggttcca gcattgggtg atcaaagcct gcaaagccag gccgtgacca tcgtccaggt
     2401 cgtgggccga aagcaccagg gccttgtcgg tggccttcag gttgcccagc actttgcgcg
     2461 cgcgttccgt ctggcggcgc ggcaaggtaa acacgcccgg cgcgatggcg ccgaacccgg
     2521 cccagatcag ttcgcggcgc aattggccac gttgatccag gctgccattg ccgaagcgcg
     2581 gcacgatcac caacgtccag tcaccctccc agtccgtggc ggccccctcg tagatgcggg
     2641 cggacgcttc gcgggtgagc gcctcgccct gcggactgag acgatacaga ctgcggcgcc
     2701 cgtggcgctc ggaccgcagc cagccctggg ccaccaggcg gaacacgcta gtgcgcaaca
     2761 ggcgttcatt gatacccaat ggggtgacca gttcgatcag atcgctgagc cacacttgcc
     2821 cgccgtgcgg gccgatggca tcgcccagaa gcgtcacgca caaggacttc gcccggggtg
     2881 gatgctgttc aagcagagcg gaaaccagag agttcggcgt gcgggagaga gtgtccatgc
     2941 gcggtcatga atgccgggag atgggtgaat ggatcagata tgcccgcccc ggtgcattcc
     3001 gttcgggtat ctgatcgata tgataccgat ttttgattga cttagatatt tttgtattaa
     3061 ataataaagc aatcgcctta tgcgccgtgt gagccaggag accatcatgt atgcccagct
     3121 agtcgatacc ggggttgcca aggtgcgtga tgccgccacg ctgtccgggc cggaaaaggc
     3181 attccagcaa cgcgtggatg acggcattcg catcgagccc aaagactgga tgccctccgc
     3241 ctatcgcaag acactggtgc gccagatatc gcagcatgcc cattccgaga tcgtcggcat
     3301 gctgccggaa ggcaactgga tcacacgggc gccgtccctc aagcgcaagg ccattcttct
     3361 ggccaaggtg caggacgagg ccggccatgg cctgtatctg tacagcgccg ccgaaaccct
     3421 gggggtgtcg cgcgatgacc tggtcgacga tctgctgacc ggcaaggcca agtattcgac
     3481 cattttcaat taccccacgc tcacctgggc cgatatcggc atgatcggct ggctggtcga
     3541 cggttcggcc atcatcaacc agatcccgtt gtgccgttgc tcgtacggcc cgtatgcccg
     3601 cgccatggtg cgcgtctgca aggaagaatc gttccatcag cgccaggggt acgacctgct
     3661 catccagatg tgccggcatg gcaccgccgc acagaaagac atggcgcagg atgcgctgaa
     3721 ccgctggtgg tggccggccc tcatgatgtt cggcccgccc gatgccgaat cggtcaatag
     3781 cgcccagtcc atgcaatggc gcatcaag
//
LOCUS       DASTNK010000185        17363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_178546, whole genome shotgun sequence.
ACCESSION   DASTNK010000185 DASTNK010000000
VERSION     DASTNK010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 17363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17363
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178546"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..130
                     /locus_tag="VFR20_13500"
     CDS             <1..130
                     /locus_tag="VFR20_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1993 family protein"
                     /protein_id="HEU0231576.1"
                     /translation="YTAQSYLLTYGMPQFFFHITTAYAILRHNGVVVGKRDFLGKF"
     gene            complement(332..973)
                     /gene="maiA"
                     /locus_tag="VFR20_13505"
     CDS             complement(332..973)
                     /gene="maiA"
                     /locus_tag="VFR20_13505"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEU0231577.1"
                     /translation="MNLYTYFRSSASFRVRIALNMKGLPYTSTPVHMLRHGGEQHLPA
                     YRAVNPLGLVPSLALDDGQVLTQSLAICEYLEETHPDPALLPATALDRAWVRSLCADI
                     ACDIHPLDNLRVLRYLKHELNCDDAQRDAWYGHWIRTGFEPLESVLRGRAGRHCLGND
                     FTLADAFLVPQVWNARRFKCAMDDFPTISRLYENAMALDAVQRAEPSQQPDAE"
     gene            1246..1704
                     /locus_tag="VFR20_13510"
     CDS             1246..1704
                     /locus_tag="VFR20_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010110174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEU0231578.1"
                     /translation="MSLNSVREFFAQHAPDISIIELDTNTATVALAAAAHGVAPGQIA
                     KTLSLRVADRAVLVVLSGDARMDNKKLRQTFGGKATMLDAESVLTLTGHPVGGVCPFG
                     LATDLPVYCDVSLRQYDIVMPAAGGIHTAVRIAPTRLAELVSAQWVDVAR"
     gene            2110..3306
                     /locus_tag="VFR20_13515"
     CDS             2110..3306
                     /locus_tag="VFR20_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0231579.1"
                     /translation="MTSYGWKALWGSAIGYAMDGFDLLILGFMLTAISADLHLTRGQA
                     GSLVTWTLVGAVCGGIIFGSLSDHFGRVRVLTWTILVFAVFTGLCAFAQGYWDLLAYR
                     TIAGIGLGGEFGIGMALAAEAWPAKHRARVSSYVALGWQAGVLLAALLIPLLLPHLGW
                     RGMFLVGIIPALIAWAIRNRLHEPELFVRKTEKRRASLLDSYKLLVKDKRTSRISLGI
                     AILCSVQNFGYYGIMIWMPSYLAKSQGFGLTKSAFWTSVTIIGMAIGIWVFGQIADRI
                     GRKPSFILFQIGAVVMVLFYSRLSDPDTMLWAGALMGVFVNGMLGGYGALMSEAYPTE
                     ARATAQNVLFNIGRAVGGFGPVVVGTLAATYSFHVAIGLLAAIYVIDMIATIFLIPEL
                     KGAELN"
     gene            3428..4279
                     /locus_tag="VFR20_13520"
     CDS             3428..4279
                     /locus_tag="VFR20_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEU0231580.1"
                     /translation="MNAVMEGFQRNADAVASHACEVVEHRWVNDRYKYLKLMAGADLA
                     AATRAGQFYQLRCPVSDTLQPFLLRPMSVYGTGPEPGGIEFLYNVTGTGTRALANLVV
                     GDVMDIVGPLGNTFTFRPAFRRILLVARGVGLATMAPVVHLAARAGVAVVAVMSARAP
                     QDLMRDEFLRGVSAEVHGLYDSDGSSEVANVESLVRGLLDHQPFDAVYTCGSHRLLLL
                     LQRVLRDYPGVLGEVAMEQHMACGMGVCLSCVRLFEVDGEKQFLRVCREGPVFPIHDV
                     VGEVRYG"
     gene            4272..5222
                     /locus_tag="VFR20_13525"
     CDS             4272..5222
                     /locus_tag="VFR20_13525"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU0231581.1"
                     /translation="MVDMGVDVGGIRLRNPVMPASGCFAIEYSEALDFNTLGALVVKS
                     VSPKSRPGNPTPRVAETASGMLNSIGIPSKGLDYYLNHVLPPYLRFDTPVVVSISADT
                     VDEFAHAAARVSVPGVAAIEANISCPNLEADGLAFAMDPDATLKALRAIRASTALPLW
                     AKLTPNTSHVADIARAAEAGGADALVMGNTVLGMSIDVHTRKPRLGNVMGGLSGPAIK
                     PIALRMVYQCYRSVDIPVIGCGGICSADDVVEFLLAGASAVQVGTASFLDPGVMPKLV
                     AGLERFCSEASVKSVRSLVGGIALDSGLTDSWLRMAQQSG"
     gene            5302..6558
                     /locus_tag="VFR20_13530"
     CDS             5302..6558
                     /locus_tag="VFR20_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEU0231582.1"
                     /translation="MTNLIQYRSLAEKLFSDVRELSFDGVGVTRDSYGRGESGTADYL
                     REFALSEGLEVSADAAANLVFRFPDVPPEAPVVWMGSHMDSVPQGGNYDGLAGVVAGL
                     LCMVARKRDGRSGAMEVIGFRGEESAWFGKACVGSTCLVGKLAEADLSLPRRGEGMTL
                     GESMRALGADVAAIAAGEKLFDVSRVKVYLELHIEQGPVMVARDLPVAVVSGIRGNFR
                     HNQVRCLGDAQHSGVVPRWLRHDAMFGVADLIMRLDEHWRVLLERGTDLVVTTGMVGT
                     DPAEHAVSRIPGQVTFSLEARSRSTDTLEAFYQLMRAECAAISRERGVRFEFDRRLVS
                     EPATMDQKVCDVLSDVCKARGLTFDVIPSGAGHDAALLANAGVPSGMVFVRNQNGSHN
                     PHEAMEIDDFMQGVQVLSDALEPLGA"
     gene            6611..7321
                     /locus_tag="VFR20_13535"
     CDS             6611..7321
                     /locus_tag="VFR20_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEU0231583.1"
                     /translation="MNQSDFGPDTLGEQPDPARAVAGALLDAGCVAVRTEEPFHLPSG
                     WASPVYMDCRRIISFPALRRQLVARGLRVLRDAGVLDGLSAVVGGEASGIALAAWFAD
                     ALELPLQYVRKRRAGHGQVEGVVSNGGKALLVDDMMAGGQSKVRFCKALASAGARVED
                     LLIVFDYDTFPTRELLAPLGVSVHALATWRDVLAVLHERGGFDPAALRDFEVFLADPA
                     GWSQAHGGLGAGPAVRNA"
     gene            7318..8238
                     /locus_tag="VFR20_13540"
     CDS             7318..8238
                     /locus_tag="VFR20_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0231584.1"
                     /translation="MTTRVRAMPDAAAFKSSIDYAALGDRLRAHRVGASLQAEDIAAT
                     LGISRAAVYRMEKGEIVKIETLERLADLLGTSLASLLGVEVEYYANALGLFERMRQLE
                     HGADRIFVHFEPISLLLTSDDYLEHLREMLLEATPRQVGGMLGSDEVDDLIRLITERR
                     ASFRARDPHVVSLIGLRGLERFLQIGMVGRVRLSSAVRQHRIEVARREVGRIAELMAS
                     EPLNVRIGIIDDAMPASTFEFFSGPERKAVAVSPFRLGELPNVRNGIATVTSAPEAVR
                     RYESLISRLWKGAYKGSAGAAQVKKLLEKF"
     gene            8408..8734
                     /locus_tag="VFR20_13545"
     CDS             8408..8734
                     /locus_tag="VFR20_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0231585.1"
                     /translation="MTFKEITQRFARSESGSDSFKQFYKDAFDLMRRDPDNAGLYFVV
                     GIAAQTYVIKYEDQAVDPDFADRVKVTLEGFNSRLLAALDMAPADRLRVLGEVAVDYE
                     WNEDAF"
     gene            complement(8818..9627)
                     /locus_tag="VFR20_13550"
     CDS             complement(8818..9627)
                     /locus_tag="VFR20_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU0231586.1"
                     /translation="MLLVSFLGALIGLVMGMTGAGGGILAVPALVAGLGLTMTAAVPI
                     SLMAVGVSALVGALDGLSKKIVRYRAAALMAVLGSLMSHAGIALAHVLPEAVLMTLFA
                     LVMLLAAWRMAHKGHGATSVAMAESTKNCMLNPHTGKLRWTPLCAATLASIGATTGLF
                     AGLLGVGGGFIVVPAFKRFTNVDMHSIVATSLTVIALISIGTVASLLARGTHISTTGW
                     VFVAAAVVGMIAGRSIAAHMPARLLQCVFSGIAVVVAIILLCKTYLPGLLA"
     gene            9928..10254
                     /locus_tag="VFR20_13555"
     CDS             9928..10254
                     /locus_tag="VFR20_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HEU0231587.1"
                     /translation="MRTSTRTFLIAAALSGFMAAPVFAATPAASHTKTAQQERMTMCT
                     KEAKGKKGAEHKAFMKSCLKGEKTGHSGKKLTASQERMRTCSKESKGKKGAEHKAFMK
                     SCLKAK"
     gene            complement(10279..13173)
                     /gene="ppc"
                     /locus_tag="VFR20_13560"
     CDS             complement(10279..13173)
                     /gene="ppc"
                     /locus_tag="VFR20_13560"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU0231588.1"
                     /translation="MNQIVPPADPSLLLRDDIRLLGKMLGEVVRHCEGRHIYDVIEKL
                     RRAAVKFRREGNARDGALMERKIHRLEDSEALSVIRAFTYFLHLSNIAEDRDQNRRQR
                     DLMRDGVAPQGTLRHTLEALRQRGVGPRKILRFLEGACIVPVLTAHPTEVQRKSTLSL
                     HRDIALSLVLLDAQPTPGERAELNQRLAGLITTLWQTRLLRRQRLAVLDEIDNALSYY
                     DSTFLTAIPRLYQDLAGALHGTERSVFDTPDASLPAFLRMGSWIGGDRDGNPNVDATT
                     LEQALLRQSTCIVRHYLRELKTLGTELSISQRLGAASPALLALAKESQDTSPHREDEP
                     YRRACIHLYARMAATGNLLTNNNLAQHPTYEAPPYPNVDAFLDDLRVMADSLEHHGGG
                     LVGQLRLSHLIQAASVFGFHLATTDLRQSSDVHERVLTELFKTAGVRRGGRVVNYAAL
                     DEDDRVALLREELRQVRPLASPWITYSAETRKELGILRAAAEGRRRYGPRAIEQTVVS
                     HTETLSDLLEVLVLQQETGLIIPSVVQGKAGQEQNRVGHDQGLMVVPLFETIPDLERG
                     PDIMAAWLDLPEVAARVRHTQGGIQEVMLGYSDSNKDGGYMTSNWSLYKAERSLVDVF
                     RKRRVRLRLFHGRGGSVGRGGGSSFDAIQAQPPGTVDGQIRLTEQGEVIQSKYKDAEV
                     GRWHLELFVAATLESSLTTGRGASQSEDENMARHGGTAAFLSEVAEATYRQLVYQTPG
                     FTDYFFASTPIREIAGLNIGSRPASRKASQRIEDLRAIPWGFSWAQCRLMLTGWYGMG
                     TALNAYLERGGPDAPNGKTGAGKAARLAELQAMAREWPFFRTLLSNMEMVLAKTDLAI
                     GRRYASLVPDAALRARVFDAIETEHALTVDMFNKVAQRGLLDDNLQLRAALSERFAYI
                     DPLNHLQVELLRRHRAADAPAADQGGEESRIQRAIHMTINGIAAGLRNSG"
     gene            13630..15522
                     /gene="ilvD"
                     /locus_tag="VFR20_13565"
     CDS             13630..15522
                     /gene="ilvD"
                     /locus_tag="VFR20_13565"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU0231589.1"
                     /translation="MPQYRSRTSTHGRNMAGARALWRATGMTDGDFDKPIIAVVNSFT
                     QFVPGHVHLKDLGQLVARQIEAAGGVAKEFNTIAIDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNGHCADAMVCISNCDKITPGMLMAAMRLNIPTVFVSGGPMEAGKVIAASDGK
                     VVMKLDLIDAMIQAADPNVSDEQAAEVERSACPTCGSCSGMFTANSMNCLAEALGLAL
                     PGNGSLLATHARRKGLFERAGQLIVALCKRYYEHDDVSVLPRSIATHQAFENAMMLDV
                     AMGGSTNTVLHLLAAAQEAEVAFTMADIDRISRRVPCLSKVAPATNKYHMEDVHRAGG
                     VIGILGELARAGLLNLDVGNVHSGTLRQAIHAWDVNSGHATPEVQDFYKAAPGGVRTT
                     EAFSQNRHYDSLDVDREHGCIRDKAHAYSQDGGLAVLYGNLALNGCIVKTAGVDDSIL
                     VFKGRARVYESQEASVGGILGGAVQAGDVVVIRYEGPKGGPGMQEMLYPTSYLKSMGL
                     GKGTALLTDGRFSGGSSGLVLGHASPEAAEGGNIGLVRDGDTIEIDIPGRTIHLAISD
                     EELQARRDEMDARGDQAWQPVNRERQVSQALRAYAALATSADRGAVRDVSQLTRAKKP
                     SWVEAD"
     gene            15676..17361
                     /gene="ilvD"
                     /locus_tag="VFR20_13570"
     CDS             15676..17361
                     /gene="ilvD"
                     /locus_tag="VFR20_13570"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU0231590.1"
                     /translation="MSSPKRLRSAHITQGVERAPNRSMYYALGYTESDFSKPMVGIAN
                     GQSTITPCNSGLQPLADAAAQAVKKAGGNPQMFGTPTISDGMAMGTEGMKYSLISREV
                     ISDCVETCVQGQWMDGVLVIGGCDKNMPGGMMGMLRANVPAIYVYGGTILPGHYKGQD
                     LNIVSVFEAVGENAAGRMSAEDLRQIELHAIPCSGSCGGMYTANTMSSSFEALGMSLP
                     YSSTMANVHEEKLASAAESARVLLRAIELDLKPRDIVTRASLENAVSVVMATGGSTNA
                     VLHLLAIAHAAGVEWTIDDFERVRRKVPVLCDLKPSGKYLVVDFHKAGGIPQVMKLLL
                     NAGLLNGDCMTITGQTIAETLRDVPDVPSKDQDVIHTVDDPMYAQGHLAILKGNLATE
                     GAVAKITGLKNPVMTGPARVFDDEQSALKAILDGRVKAGDVMVLRYLGPRGGPGMPEM
                     LAPTSALIGAGLGDSVGLITDGRFSGGTWGMVVGHVSPEAAVGGGIALVKEGDSITID
                     AHQHLLQLNVAEDELASRRKQWQAPKPRYMRGVQAKFAFNCSNASTGAVLDNF"
BASE COUNT         2972 a         5249 c         5816 g         3326 t
ORIGIN      
        1 ctataccgct cagtcttacc tgttgaccta cgggatgcca cagttcttct ttcacatcac
       61 gacggcttac gccatcctgc gccacaacgg cgtagtggtc ggcaagcggg attttctggg
      121 gaagttctag agggctgaat cggtcatcga gtcgcatgca tccgcggctc ggtgccagat
      181 ttttttcggc gtcagccgcg gcagcccggc cctcgcccat cctcgatcac tcagatggcg
      241 tcgttcaggg ccaccacgac atcgtggatg cgaggagggt gggtaatcat cacggccttg
      301 tgcccgcagc cattgggcgt ggtcgtgtgt tttactcggc gtcgggttgt tgcgacggtt
      361 ccgcccgctg cacggcgtcc agcgccatgg cgttttcgta caggcggctg atcgtgggaa
      421 agtcgtccat ggcgcacttg aagcgtcgcg cattccaaac ctggggcacc aggaatgcgt
      481 cggccaacgt gaaatcattc cccaggcagt gccggcccgc gcggccgcgc agcacgcttt
      541 ccagcggctc gaacccggtg cggatccagt gtccgtacca cgcatcgcgc tgggcgtcgt
      601 cacagttgag ttcgtgcttg aggtaacgca gcacgcgcag gttgtcgagc gggtggatgt
      661 cgcacgcgat gtcggcgcag agggatctca cccaggctcg gtcgagcgcc gtggcgggca
      721 gcagcgccgg gtcgggatgg gtttcttcca gatattcgca gatggccagg gattgggtca
      781 gcacttggcc gtcgtccagc gccaacgagg gcaccagccc cagcgggttc acggcccggt
      841 aggccggcag gtgctgttcg ccgccatgac gcagcatgtg cacaggggtc gaggtgtagg
      901 gtagcccctt catgttgagg gcgatgcgca cgcgaaacga ggcggacgaa cggaaatagg
      961 tatacaggtt cacggggttc tccggggctg ttttctggat tctaccggac cgcccgcatg
     1021 agggcgggag ctgaataggc atggaatgcc tggcgcgtcg caatgggttg ctgggttggg
     1081 gttccgggtg aagcatccgt ttgccggcgg ccgtcgtttc agaacataat ccaagcctat
     1141 ggtcgcgtcg acccagcagc cgtgatcgtg tgcgctgtca cgggcgagct tgttcaacgt
     1201 cgctgtgctt gcgcattttt caaaccataa ccgacagtct tgctcatgag cctgaattcc
     1261 gtgcgcgagt tcttcgcaca acacgccccc gatatttcca tcatcgaact cgacaccaac
     1321 acggccaccg tcgcgctggc ggcggccgcg catggggtgg cgcccgggca gatcgccaag
     1381 accctgtcgc tgcgtgtggc ggaccgggcc gtgctggtgg tgctcagcgg tgacgccaga
     1441 atggacaaca agaaactgcg ccagacgttt ggcggcaagg ccacgatgct cgatgcggag
     1501 tcggtgctga cgctgaccgg ccacccagtg ggcggtgttt gtccgttcgg gctggcgacg
     1561 gatctgcctg tctactgtga cgtgtcgctg cgacagtacg acatcgtcat gcccgccgcc
     1621 ggcggcatcc ataccgccgt gcgcatcgcc cccacccggc tggcggaact cgtttccgct
     1681 cagtgggtcg acgtcgcccg gtgattcggg ctttttccac tcccgcgtcg agatgccatt
     1741 gacctcagcg catcccgctg atcgcattgc atcgcgggtg ggacggcatc cttcctagtg
     1801 aaactaccta gttgttttca gggaatttgc agcggtttgt ctaaaaaaat agattaatat
     1861 ataaaaagat attccacatc gatggcggac ttgcttttcc gtctttcagt gagtcggggc
     1921 gacccccggg caccacatgg ccgcaatgaa atcaatcggt ggcggggccg ctgccccggt
     1981 tccatccggc acgcacctcc ggcgcaaacg cttcgcctga accctcaaca tttttcacca
     2041 tacaaaggga gcaacacatt ggaagctcga ctcaattccg ggagcggtga cccagtcgat
     2101 gacaccgccg tgacgtccta cggatggaaa gccctgtggg ggtccgccat tggctatgcc
     2161 atggatggct tcgacctgct catcctcggt ttcatgctga cggcgatctc ggccgacttg
     2221 catctcacgc gcgggcaggc cgggtccctg gtcacctgga cgctggtggg cgcggtgtgc
     2281 gggggcatca tcttcggctc gctcagtgat cacttcggcc gtgtgcgggt tctgacctgg
     2341 accattctgg tcttcgccgt gttcaccggg ctgtgcgcgt tcgcacaagg gtattgggac
     2401 ctcctggctt accgcaccat cgccggcatc ggcctgggcg gcgagttcgg catcggcatg
     2461 gccctggccg ccgaggcctg gccggcgaag catcgcgcac gggtctcgtc gtatgtcgca
     2521 ctaggctggc aggcgggggt gctcctggcc gcgttgctga ttccgctgtt gttgccacat
     2581 ctgggctggc gcggcatgtt cctggtcggc atcatcccgg cgctcatcgc ctgggccatc
     2641 cgcaatcggc tgcatgagcc cgaacttttc gtgcgcaaga cagaaaagcg ccgcgccagc
     2701 ctgctggatt cgtacaagct gctggtcaag gacaagcgca cctcgcgcat cagcctgggc
     2761 atcgccatac tgtgttcggt gcagaacttc ggctactacg gcatcatgat ctggatgccg
     2821 agctatctgg cgaagtcgca ggggttcggg ttgaccaagt cagccttctg gacgtccgtg
     2881 accatcatcg gcatggcgat cgggatctgg gtgtttggcc agatcgccga tcgcatcggc
     2941 cgcaagccca gcttcattct gttccagatc ggggctgtcg tgatggtgct gttctattcc
     3001 cggctgtccg acccggacac catgctctgg gccggggccc tgatgggggt gttcgtcaac
     3061 ggcatgctgg gcggttacgg cgcgctgatg tccgaagcgt atcccaccga ggcgcgcgcc
     3121 acggcgcaga acgtgctgtt caacattggc cgggcggtgg gcggattcgg tccggtggtg
     3181 gtgggcacat tggccgcgac atattcgttc cacgtcgcga tcggcctact ggcggccatc
     3241 tacgtcatcg acatgatcgc caccattttc ctgatccccg agctgaaggg ggcggagctg
     3301 aattgatgtg cactgatgtg gacggagctg gatcgatgga tatggatggt ggcggagcgg
     3361 ggctgatgcc caccgacgtg gtcttgggcc gcgcggtctt gccctcctcg gagatcgggg
     3421 gtggcgaatg aatgcggtga tggaaggctt ccagcgcaac gccgacgcgg tcgcctcgca
     3481 cgcctgcgag gtggtggaac accgctgggt gaatgatcgc tacaagtacc tcaagctgat
     3541 ggccggcgcc gatctggcgg ccgccacccg ggccggccag ttctatcagt tgaggtgccc
     3601 ggtgtcggac accttgcagc cattccttct gcgccccatg agcgtctatg gcacggggcc
     3661 tgaacccggt ggcatcgagt tcctctacaa cgtgacgggc accggtaccc gcgcgctggc
     3721 gaacctggtg gtaggcgacg tcatggatat cgtggggccg ctgggcaaca cattcacgtt
     3781 tcggccggcg tttcgacgca ttctgctggt ggcgcggggt gtgggtctgg caaccatggc
     3841 gccagtcgta cacctcgcgg cacgagccgg tgtcgccgtg gtggcggtga tgtcggcgcg
     3901 tgcgccgcaa gatctgatgc gtgacgagtt cctgcgcggc gtgtcggccg aggtgcatgg
     3961 tttgtatgac agcgatggtt cgtccgaggt ggcgaacgtc gaatccctgg tgcgcggact
     4021 gctcgatcat caaccctttg atgcggtcta cacctgcggg tcgcatcgat tgttgttgct
     4081 gcttcagcgg gtcctgaggg attatcccgg cgtgttgggg gaagtggcga tggaacagca
     4141 catggcctgc ggcatggggg tctgcctgtc gtgcgtgcgg ctgttcgagg tcgatgggga
     4201 aaagcagttc ctgagggtgt gccgcgaagg cccggtgttc cccattcacg atgtggtggg
     4261 tgaggtccgc tatggttgac atgggcgtag acgtcggcgg catccgattg cgcaacccgg
     4321 tgatgccggc ttcgggctgc ttcgccatcg agtacagcga ggcattggac ttcaacacgc
     4381 tgggcgccct ggtggtcaag agtgtgtcgc caaagagccg gccgggcaac ccgacgccgc
     4441 gcgtggcgga aaccgcatcg ggcatgctca attccattgg tatcccgagc aagggtctcg
     4501 actactatct gaaccacgta ttgcctccgt acctgaggtt cgacacaccc gtggtggtgt
     4561 cgatatcggc cgatacagtg gacgaattcg ctcacgccgc ggcccgggtt tccgtgccgg
     4621 gcgtggccgc catcgaggcc aacatctcct gcccgaacct cgaggccgac gggctggcgt
     4681 tcgccatgga tccggacgcc acgctgaagg ctctgcgcgc catccgcgca agcacggcat
     4741 tgcccctgtg ggcaaaactc acgcccaaca cctcgcacgt cgccgacatc gcgcgtgccg
     4801 ccgaggcggg gggcgccgac gcgttggtca tgggcaacac ggtgttgggc atgtccatcg
     4861 acgtgcatac gcgcaagccg aggctgggca atgtcatggg cgggctatcg gggcccgcca
     4921 tcaagcccat cgccctgcgc atggtttacc aatgctatcg cagcgtcgat attcccgtca
     4981 tagggtgtgg tggcatctgc agcgctgacg acgtggtgga gttcctgctg gcgggtgcca
     5041 gcgcggtgca ggtgggcacg gcatcgttcc tggatccggg cgtcatgccc aagctcgtcg
     5101 ccggattgga acgattctgc tcggaagcct cggtgaagtc tgtgcgcagc ctggtgggcg
     5161 gcatcgcgct cgactccggc ctgacagaca gctggctgcg catggcgcag caatctggtt
     5221 gatcccgcgt tcaacgatgc gacgtacttt tggaatccac tcactccgaa tggaacctgg
     5281 cattgacggc tttgcaccat catgacgaat ctgatccagt accgatcctt ggcggaaaaa
     5341 ctcttcagcg atgtgcgcga actgtctttc gacggtgtcg gcgtcacgcg cgacagctat
     5401 gggcgcggcg aatcgggcac ggccgactat ctgcgagagt tcgcgttgtc ggaaggtctg
     5461 gaggtgtcgg ccgacgcggc ggccaatctg gtgtttcgtt tccccgatgt gccgcccgag
     5521 gcgccagtgg tctggatggg gtcgcacatg gattccgtcc cgcaaggcgg caattacgac
     5581 gggttggccg gggtcgtggc gggcctgttg tgcatggttg ccaggaagcg cgacggccga
     5641 agcggcgcca tggaggtcat cggttttcgc ggagaggaaa gcgcgtggtt cggcaaggcc
     5701 tgtgtcgggt ccacctgcct ggtgggcaag ctggccgagg ccgacctgtc gctgccgcgg
     5761 cgtggggaag ggatgacgct gggcgagagc atgcgggcgc tgggcgccga cgtggcggcg
     5821 atcgccgctg gcgaaaaact gttcgacgtg tcccgtgtca aggtctacct ggaattgcac
     5881 atcgagcagg ggccggtcat ggtggcgcgc gatctgcctg tcgccgtggt gtcgggcata
     5941 cgcggaaact tccgccacaa ccaggtgcgg tgcctgggcg atgcgcagca ctcgggtgtg
     6001 gtgccgcgct ggctgcgcca cgacgccatg ttcggtgtgg ccgatctcat catgcggttg
     6061 gacgagcact ggcgcgtgct gctggagcgg ggtaccgatc tcgtcgtcac gacggggatg
     6121 gtgggcaccg atccggcgga acacgcggtg tcgcgcattc ccggacaagt gacgttcagc
     6181 ctggaggcgc gcagtcgaag caccgatacg ctcgaggcgt tctatcagct catgcgcgct
     6241 gaatgcgcgg ccatttcccg cgagcgcggc gtgcggttcg agttcgacag gcgcttggtc
     6301 agcgaacccg ccaccatgga tcaaaaagtg tgcgatgtct tgtcggatgt atgcaaggcg
     6361 cgcggcctca cgtttgatgt gatccccagt ggcgccggcc atgatgcggc actgctcgcg
     6421 aatgccggag tgcccagcgg catggtgttt gtgcgaaacc agaatgggtc gcacaatccc
     6481 catgaggcga tggagatcga tgatttcatg cagggggtgc aagtattgag cgacgcgctc
     6541 gagccactgg gcgcatgatg tcgcgtctcc cgagtgcgtg ttccaaccag agtcaaacaa
     6601 agaggcagac atgaaccagt ccgatttcgg ccccgacacg ctgggcgaac agcccgatcc
     6661 ggcgcgggcc gtggccggtg ccttgctcga tgcggggtgt gtggcggtgc gaaccgaaga
     6721 gccgtttcat cttccctcgg gctgggcgag cccggtctat atggattgtc gtcgcatcat
     6781 ttcctttccg gccttgagac gccagctcgt cgcgcgcggc ctgcgtgtat tgcgcgacgc
     6841 cggtgtgctc gatggcctgt cggcggtcgt cgggggcgag gccagcggga tcgcgttggc
     6901 cgcatggttc gccgacgcgc ttgaattgcc cctgcagtat gtgcgcaagc gccgggccgg
     6961 ccacgggcag gtcgaagggg tggtgtcgaa cggcggcaag gcgctgctgg tcgacgacat
     7021 gatggcgggc gggcagtcca aggtgcggtt ctgcaaggcg ctggcgtcgg cgggggcccg
     7081 cgtcgaggat ctgcttatcg tgttcgacta tgacacgttt cccacccgtg aactgctcgc
     7141 gccgctgggc gtgtcggtgc atgcgttggc cacttggcgc gatgtgctgg ccgtgctgca
     7201 tgagcgtggc gggttcgatc cggccgcttt gcgcgacttc gaagtgtttc ttgccgatcc
     7261 ggcggggtgg tcgcaggcgc atggcgggct gggagccggc cccgcggtgc ggaacgcgtg
     7321 acgacgcgcg ttcgagccat gcccgacgcc gcggcattca aatcgtcgat agactatgcc
     7381 gcgctgggtg acagactgcg cgcgcaccgc gtcggcgctt ctctgcaggc cgaagacatc
     7441 gcggcgacgc ttggcatatc ccgcgcggcc gtgtaccgca tggaaaaggg cgagatcgtc
     7501 aagatcgaaa cgctggagcg attggctgat ctgctgggca cgtcattggc gtcgctgctt
     7561 ggggtggaag tcgaatacta tgcgaacgcc ttgggcctgt tcgagcgcat gcggcagctc
     7621 gaacacgggg ccgaccgcat tttcgtgcat tttgaaccca tatcgttgtt gctgacctct
     7681 gacgattatc tggagcatct gcgcgagatg ttgctggaag ccacgccgcg ccaggttggc
     7741 ggtatgctcg gcagcgatga ggttgatgat ctcatccgtc tcatcaccga gagacgagca
     7801 tcgtttcgtg cgcgtgatcc gcatgtcgtc agcctcatcg gactgcgcgg cctggagcgt
     7861 ttcctgcaga tcggcatggt cggcagggtg cggttgtcga gtgcggtgcg tcagcatcgc
     7921 atcgaggtgg cgcgccggga agtgggccgg atcgcggaat tgatggccag cgaaccattg
     7981 aacgtgcgga tcggcatcat cgatgacgcg atgcccgcgt ccaccttcga gttcttttcc
     8041 ggtccggagc gtaaggcggt ggccgtcagt ccgttccggt tgggggaact gcctaatgtg
     8101 cgcaacggca tcgccacggt cacctcggcg cccgaggcgg tgcgccgtta cgaaagcctg
     8161 atttcccggt tgtggaaagg ggcctacaaa ggcagtgccg gggccgcgca ggtgaagaaa
     8221 ctgctcgaga agttctgatg tcgggggcct ggggtggatt ggagtcgcca tcaccggcgc
     8281 gtcgacggag gatgacgcta ccacccgccc gggtttgcgg ctattctatt tgtctgccga
     8341 cggttgtgct gatgacatcc ggcgatggca tcacaaccgc tgttgtcttg aagtggagtt
     8401 tcaggaaatg acgttcaagg aaatcacgca acgtttcgcg cgttcggaat cgggtagtga
     8461 ctcgttcaaa cagttctaca aagatgcctt cgatctcatg aggcgggatc cggacaacgc
     8521 cggcctgtat ttcgtggtgg gcatcgccgc ccagacgtac gtcatcaagt atgaagacca
     8581 ggcggtcgat cccgacttcg ccgatcgggt caaggtgacg ctcgaagggt tcaacagcag
     8641 gctgcttgcc gcgctcgaca tggccccggc cgacaggctg cgtgtgctgg gcgaggttgc
     8701 cgtcgactac gaatggaacg aagacgcgtt ttaactgttc gactatgtgt tgtttggtgg
     8761 gatggcgtac tcaacttccc gaacatgaca ccaagcgggc gaacggtggg gggtggctca
     8821 ggccaatagt cccggcaagt aggtcttgca cagcaggatg atcgcgacca ccacggcgat
     8881 gcctgagaac acgcattgca gcaggcgggc gggcatgtgc gccgcgatag accggcccgc
     8941 gatcatgccc accacggcgg ccgcgacaaa aacccagccc gtcgtggata tgtgagtgcc
     9001 tcgcgccagc aggctggcca ccgtgccgat ggaaatcagc gcgatcaccg tgagcgaggt
     9061 ggcaacgata ctgtgcatgt ccacgttggt gaatcgcttg aaggcgggca ccacgatgaa
     9121 tccgccgccc acgcccagca ggcccgcgaa caagccggtg gtggccccga tgctggcgag
     9181 cgtggccgcg catagcgggg tccagcgcaa tttgccggta tgggggttca gcatgcagtt
     9241 cttcgtggat tcggccatgg ccaccgaggt ggcgccatgc cccttgtggg ccatgcgcca
     9301 ggcagccagc agcatgacca aggcgaacag cgtcatcagc acggcttccg gcagcacatg
     9361 cgccagcgcg atgcccgcgt gggacatcaa cgatcccaac acagccatca acgcggcggc
     9421 gcgatagcgc acgatttttt ttgacaggcc atccagggcg cccaccagcg ccgagacgcc
     9481 cacggccatc aacgaaatgg gaacagccgc cgtcatggtc aggcccagcc ccgccaccag
     9541 ggcgggaacg gccagaatgc cgccgccggc gccggtcatg cccatgacca gaccgatcag
     9601 cgcgccgaga aaactgacta gtaacattct gtctgtttgg tgagagggtt gtggcaggcc
     9661 gcgcaggcgg gcgcgacgag caggcctggc gaagggttgc atcgcgccag ctcggcggcg
     9721 tttccgcgtc cagagggtat tataattgtt ttaagtaata tttatatatc ttattgttat
     9781 attggttttg tttcaaaaga atcgtcaagg tagatatcgc gcgcttgcgc ggatgtgggc
     9841 ttccagtgca agattcgcat gccggtcgtg acattttcgt ccgccgcgat tctgtccttc
     9901 ccttatctga ttactgcgag gtgaattatg cgtacatcaa cccggacgtt cctgatcgcc
     9961 gcggcgctca gcggcttcat ggccgccccc gtctttgcgg caacccccgc ggcttctcac
    10021 accaagaccg cccagcaaga gcggatgacg atgtgcacca aagaagccaa aggcaagaag
    10081 ggggctgagc acaaggcctt catgaaatct tgcctcaagg gcgagaagac cgggcacagc
    10141 ggcaagaaac tcaccgcatc ccaggaacgc atgagaacct gcagcaagga atccaagggc
    10201 aagaagggcg ccgaacacaa agcgtttatg aaatcctgcc tcaaggccaa gtaggcggct
    10261 cccgcgcaac cggggccatc accccgagtt gcgcaggcca gcggcgatac cgttgatggt
    10321 catgtgtatc gcccgctgga tgcggctttc ctcaccgcct tggtccgcag caggcgcgtc
    10381 ggcggcgcgg tgccggcgca acagttcgac ctgcaggtgg ttcagggggt cgatgtaggc
    10441 gaagcgctcg ctcagtgcgg cgcgcaactg caggttgtcg tccagcaggc cgcgctgcgc
    10501 gaccttgttg aacatgtcca ccgtcagggc gtgctcggtt tcgatggcgt cgaagacccg
    10561 tgcgcgcaac gccgcgtcgg gcaccaggct ggcgtagcgc cggccgatgg ccagatcggt
    10621 cttggccagc accatttcca tgttcgacag cagggtccgg aaaaatggcc attcgcgggc
    10681 catggcctgc aattcggcca ggcgcgcggc ctttccggcg ccggtcttgc cgttgggagc
    10741 gtccggcccg ccacgttcga ggtacgcgtt cagcgccgtg cccatgccgt accacccagt
    10801 cagcatcaag cggcactgcg cccatgagaa cccccacggt atggcccgga ggtcttcgat
    10861 gcgctggctg gccttgcgcg acgcggggcg cgacccgata ttcaggcccg cgatctcgcg
    10921 tatgggggtc gaggcgaaaa agtagtctgt gaagcctggt gtctgataga cgagctgtcg
    10981 ataggtggct tccgccacct cggacaagaa ggcggcggtt ccgccgtggc gggccatgtt
    11041 ctcgtcctcg ctctgcgagg cgccgcgccc ggtggtgagg ctggattcca gcgtggcggc
    11101 gacgaacagt tccaggtgcc agcggccgac ttcggcatcc ttgtatttgc tctggatcac
    11161 ctcgccttgt tcggtcaacc ggatctgacc atcgaccgtc ccgggcggct gggcctggat
    11221 ggcgtcgaaa ctggagccgc cgccgcgccc aaccgatccg ccccgcccgt ggaataaccg
    11281 cagccggaca cggcgctttc tgaagacatc gaccaggctt cgctccgcct tgtacagcga
    11341 ccagttcgac gtcatgtagc cgccgtcctt gttgctgtcg gaatagccca gcatgacttc
    11401 ctggatgccg ccctgggtgt ggcgcacgcg cgcggcgacc tcgggcaggt ccagccaggc
    11461 cgccatgatg tccgggccgc gttccagatc ggggatggtt tcgaacaacg gcaccaccat
    11521 gagcccctgg tcgtgcccga ctctgttctg ctcctgtccg gctttgcctt gaaccacgga
    11581 cggtatgatc aggccggttt cctgctgcag caccagcact tcgagcagat cactgagcgt
    11641 ttccgtgtgc gagacaaccg tctgctcgat ggcgcgcggg ccgtagcgcc tgcgcccctc
    11701 ggccgcggcg cggagaatcc caagctcctt gcgggtttcg gcggagtaag tgatccaggg
    11761 ggaggccagg gggcgcacct ggcgcagctc ttcgcgcagc agggcgacgc ggtcgtcctc
    11821 atccagggcg gcgtagttga cgacccggcc gccccggcgc acaccggccg tcttgaagag
    11881 ttcggtcagc acgcgttcgt ggacgtccga gctttggcgc aagtcggtcg tggccagatg
    11941 aaagccgaac acgctggcgg cctggatcag gtggctcagg cgcagttgcc ccaccagacc
    12001 gccgccatgg tgttcgagcg agtcggccat cacccgcagg tcatcgagaa acgcatcgac
    12061 gttcggatag gggggcgctt catatgtggg gtgttgcgcc agattgttgt tcgtgagcag
    12121 attgccggtg gcggccatgc gcgcgtacag atgaatgcag gcgcggcgat acggttcgtc
    12181 ttcgcggtgt ggggaggtat cctgactttc cttcgccagc gccagcagtg ccgggctggc
    12241 cgcgcccagt cgctgcgaga tcgacagttc ggtgcccaat gtcttgagct cgcgcaggta
    12301 atgccgcacg atgcaggtgg actggcgcag aagcgcctgc tccaatgtgg tggcgtccac
    12361 gttgggattg ccatcgcgat cgcctcctat ccaactgccc atgcgcagaa aggcgggcag
    12421 gctggcgtcg ggggtatcga agaccgaacg ctccgtaccg tgcagggcgc cggccagatc
    12481 ctggtacagg cgggggatgg cggtcaggaa ggtgctgtcg taatacgaca gggcgttgtc
    12541 gatttcgtcg agcacggcca agcgctggcg ccgcagcagg cgtgtctgcc atagtgtcgt
    12601 tatcaggccc gccaggcgct ggttcagttc ggcacgctcc cccggtgtgg gttgcgcgtc
    12661 cagcaagacg agcgacagtg cgatatcccg atgcaggctc agtgtgctct tgcgctgcac
    12721 ctcggtgggg tgggcggtca ggacgggcac gatgcatgcg ccttctagaa agcgcaggat
    12781 ctttcttggg ccgacgccgc gctggcgcag cgcttccagc gtgtgtcgca gcgtgccttg
    12841 cggcgccacg ccgtcgcgca tcagatcgcg ttgccgccgg ttctggtcac ggtcctccgc
    12901 gatgttggaa agatgcagga aatacgtgaa tgcccggatc accgacaggg cttcgctgtc
    12961 ttccagtcga tgtatcttgc gctccatcag cgcaccgtcg cgggcgttgc cctcgcgacg
    13021 gaatttcacc gccgcgcgcc gcagcttttc tatgacgtcg tagatgtgcc ggccctcgca
    13081 gtggcggacg acctcgccca gcatcttgcc caacaggcgg atgtcgtcac gcaacagtag
    13141 ggaggggtcg gcggggggca cgatctgatt cacggggcgt ccttgaactg tatggaaggg
    13201 tgagacaggc agtgtgcccg tcagcgggtc gtccaatact atacggcagg agcggtattc
    13261 gtgaccgcct gaaggtattt gtcgccgcga tggactgccg ttggttcaca cgtgccgccg
    13321 tgaggcttga caggacgctg tccgtcatca gcatgcgggt caaacggttt gaagatcggc
    13381 cggccatgcc gtcgaaattg gtcgatgaat aggtatcgat cgtagagttg acctcagtgg
    13441 tgagttgagt gattttgttc ggcggcgatg ctcgtcaagg ggccggaagc agcgaaatgg
    13501 cgcccaaagg cgcaaaattc ccgccgcgat tatcataatg gcgtcgtccc tcggggccac
    13561 cggtccgccg gcggtgcgca gggcctcgct ccggttcgtt tcccgcaact tctttttcag
    13621 agaatttcga tgccgcaata ccgttcccgc acctcgaccc acggacgcaa catggctggc
    13681 gcccgcgcgc tctggcgcgc caccggcatg acagatggcg atttcgacaa gcccatcatc
    13741 gccgtggtca actcgttcac ccagttcgtg ccgggtcatg tgcacttgaa agacctgggg
    13801 cagctcgtgg cgcgccagat cgaagccgcc ggcggcgtcg ccaaggaatt caacaccatc
    13861 gccatcgacg acggcatcgc catggggcac ggtggcatgc tgtattcgct gccctcgcgc
    13921 gaactgatcg ccgattcggt cgaatacatg gtcaatggcc actgcgccga cgccatggtg
    13981 tgcatctcga attgcgacaa gatcaccccg gggatgctga tggccgccat gcgcctgaac
    14041 attcccaccg tattcgtctc gggcgggcca atggaggcgg gcaaggtcat tgccgccagt
    14101 gatggcaagg tggtgatgaa gctcgacctc atcgacgcca tgatccaggc ggcggacccc
    14161 aacgtgtccg atgagcaggc ggccgaagtc gagcgcagcg cctgccccac ctgtggttcg
    14221 tgctcgggca tgttcaccgc caactccatg aattgcctgg ccgaggccct gggtctggca
    14281 ctgccgggca acggctcgtt gttggccacg catgcccgcc gtaaaggctt gttcgagcgc
    14341 gcggggcagc ttatcgtcgc gctgtgcaag cgctattacg aacatgatga tgtgtcggtc
    14401 ctgccgcgca gtatcgcgac ccaccaggcg ttcgaaaacg ccatgatgct cgacgtcgcc
    14461 atgggcggtt ccaccaacac ggtgctgcat ctgctggcgg cggcccagga agccgaggtg
    14521 gcgttcacca tggccgacat cgaccgcatc tcgcgccgcg tgccatgcct gagcaaggtc
    14581 gcgccggcca ccaacaagta ccacatggaa gacgtgcatc gcgccggtgg cgtgatcggc
    14641 attctgggtg aactggcgcg cgccgggctg ctcaatctgg atgtgggcaa tgtccacagc
    14701 ggcacgttgc gccaagccat ccatgcctgg gacgtcaaca gcgggcacgc gacacctgag
    14761 gtccaggatt tctacaaggc cgccccgggc ggtgtgcgca ccaccgaggc gttcagccag
    14821 aatcgccatt acgacagtct ggacgttgac cgcgaacacg gctgcatccg cgacaaggcc
    14881 cacgcttatt ctcaggatgg cgggctggcg gtgctgtatg gcaatctcgc gctcaatggc
    14941 tgtatcgtca agaccgccgg ggtggatgac agcattttgg tcttcaaggg gcgcgcccgc
    15001 gtgtacgaga gccaggaggc gtcggtgggc ggcattctgg gcggcgcggt gcaggccggc
    15061 gacgtggtcg tcatccgcta cgagggcccc aagggcgggc ccggcatgca ggaaatgctc
    15121 tacccaacgt cctacctgaa atccatgggg ctgggcaagg gcaccgcctt gctgaccgac
    15181 ggccggttct ctggcggttc gtcagggttg gttctgggcc acgcctcgcc cgaagccgcg
    15241 gagggcggca atatcggctt ggtgcgggat ggtgacacca tcgaaatcga catccccggc
    15301 cgtaccatcc accttgcgat cagcgacgag gaattgcagg cgcgacgcga cgaaatggat
    15361 gcgcgcggcg accaggcgtg gcagccagtg aaccgcgaac gccaggtatc gcaggctctg
    15421 cgggcctacg cggcgctggc cacgtccgcc gatcgtggtg ctgtgcgcga tgtgtcgcaa
    15481 ctgacgcgcg cgaagaagcc atcgtgggtc gaggccgact gatcgccccg cgcggggttg
    15541 cccctgctgt gggcagggtt cggcctcgtt gctcgccagc agtggatgta cacttgttcg
    15601 ggtagcgccg accggcggct catcccgccg gccctggcga cttgttcctg acacgttgct
    15661 tcccggaggt taagcatgag ctcgccgaaa cgcctgcgtt ccgcacacat cacccagggc
    15721 gtggagcgtg cccccaatcg ctccatgtac tacgcgctgg gctacacaga atctgatttc
    15781 tccaagccca tggtaggtat cgccaacggg caaagcacca tcaccccctg caacagcggc
    15841 ctgcagccgt tggccgatgc cgccgcccag gcggtcaaga aggctggcgg caatccgcag
    15901 atgttcggca cccccacgat ctccgatggc atggccatgg gcaccgaggg catgaagtat
    15961 tcgctgatct cgcgcgaggt gatttccgat tgtgtggaaa cctgtgtgca gggccaatgg
    16021 atggacggcg tcctggtcat aggtggctgc gacaagaaca tgcccggcgg catgatgggc
    16081 atgctccgtg ccaatgtgcc cgccatctat gtctacggcg ggaccatcct gccggggcac
    16141 tacaagggcc aggacctgaa cattgtcagc gtgttcgaag ccgtgggcga gaacgcggcg
    16201 gggcgcatgt cggccgaaga cctccgccag atcgaactgc acgccatccc ctgcagcggc
    16261 tcgtgcggcg gcatgtacac ggccaacacg atgagctcgt cgttcgaggc cctgggcatg
    16321 agcctgccgt attcctcgac catggccaat gtgcacgagg aaaaactggc gtcggcggcg
    16381 gaatcggccc gggtgctgct gcgcgccatc gaactcgatc tcaagccgcg cgacatcgtc
    16441 acgcgcgcgt ccctcgagaa tgccgtcagc gtggtcatgg ccacgggcgg ctccaccaat
    16501 gccgtgctgc atctgctcgc catcgcccat gccgcggggg tcgagtggac catagacgat
    16561 tttgagcgtg tgcgccgtaa agtgccggtg ctttgcgacc tgaaacccag cggcaagtat
    16621 ctggtggtcg acttccacaa ggcgggcggc attccccagg tgatgaagct gttgctcaac
    16681 gccgggttgc tcaacggcga ctgcatgacc atcaccggcc agaccattgc tgaaacattg
    16741 cgcgacgtgc cggatgtgcc gtccaaggat caagacgtca tccacacggt ggatgacccc
    16801 atgtatgccc agggccacct ggccatcctc aagggcaatc tggccaccga gggcgcggtg
    16861 gccaagatca ccggtctcaa gaaccccgtc atgacggggc cggcccgcgt gttcgacgac
    16921 gagcaatcag ccctgaaggc catcctcgat ggccgcgtca aggcgggcga cgtcatggtg
    16981 ctgcgctacc tggggccgcg cggcggtccc ggcatgccgg aaatgctggc gcccaccagc
    17041 gccctgatcg gtgccgggct gggcgattcg gtcgggctca tcaccgatgg ccggttctcg
    17101 ggcggtacct ggggcatggt ggtggggcat gtgtcgcccg aggcggctgt gggcggcggc
    17161 attgccctgg tgaaagaggg cgattccatc accatcgacg cgcaccagca cctgttgcag
    17221 ctcaatgtgg cggaagacga actggcttcc aggcgcaaac aatggcaggc gcccaagccc
    17281 cgctatatgc gtggtgtaca ggccaagttc gcgttcaact gctcgaatgc gagtacaggg
    17341 gcggtgctgg ataatttcta gcg
//
LOCUS       DASTNK010000186         7923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1656397, whole genome shotgun sequence.
ACCESSION   DASTNK010000186 DASTNK010000000
VERSION     DASTNK010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7923
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656397"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VFR20_13575"
     CDS             complement(<1..855)
                     /locus_tag="VFR20_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU0231591.1"
                     /translation="MRLIKKALTFDDVLLVPAFSEVLPRDTSLVTHFTRDITLNIPLV
                     SAAMDTVTESRLAIAMAQEGGIGIIHKNLTADEQAREVARVKRHEFGIVIDPVTVTPT
                     MKVRDAIALQRQHGISGLPVVEAGKLVGIVTNRDLRFEDRLDVPLRDIMTPQERLITM
                     KEGATLDEAQSLMHKHRLERVLIVNDKFQLRGLATVKDIVKNTEHPIACKDAQGQLRV
                     GAAVGVGEGTEERVEKLAAAGVDVIVVDTAHGHSAGVIERVRWVKKNYPKIQVVGGNI
                     ATAAAAKAL"
     gene            complement(1014..5072)
                     /gene="purL"
                     /locus_tag="VFR20_13580"
                     /gene_synonym="purI"
     CDS             complement(1014..5072)
                     /gene="purL"
                     /locus_tag="VFR20_13580"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEU0231592.1"
                     /translation="MTSIQILPGSPALSAFRRERLLQRLARLELPISGVHASYEHFVW
                     LGAALNTGDRDRLVALLDYGERAAVADTGRHDLVLRVIPRPGTVSPWASKATDIAHNC
                     GLSSVRRVERGIRYILTPNRGLLGVRKIEPDQLAAIAACLHDRMTESVVDADFDGQAL
                     FESLPGKPTRRVAVLEQGRDALLRANRELGLALSDDEIDYLQSSFSQLGRDPTDVELM
                     MFAQANSEHCRHKIFNAQWVVDGVEQEHTLFGLIRATHAAQPKGTVVAYSDNAAVMAG
                     GPVTQFQARPPLAASPDDACVYERREVVAHTLMKVETHNHPTAIAPFPGAATGAGGEI
                     RDEGATGRGSKPKAGLTGFTVSNLRFDDAPEPWETADLPVPPARIATALDIMIDGPIG
                     GAAFNNEFGRPNLLGYFRSHEQRAGGRVWGYHKPIMIAGGLGTIDDTQSHKDPIPPGA
                     LLIQLGGPGMRIGMGGGAASSMSVGANSADLDFDSVQRGNPEMQRRGQEVIDRCWQQG
                     AGNPIVAIHDVGAGGLSNAFPELVNDAGRGAIFDLAQVHVEESGLSAAEIWSNESQER
                     YVLAILPQDLARFDVIARRERCPYAVVGVATEDRRLKVTRGGGIPGVSGSVPEAASAD
                     DIIPVDVPVDVILGKPPRMTRDVRREPGAGESLDLSAVSLEEAALRVLRHPTVANKTF
                     LVTIGDRTVGGLSSRDQMVGPWQVPVADCAVTLSGFETTRGQAMAMGERTPVAVIDAA
                     ASGRLAVAEALTNLIAADVGSLHDIKLSANWMAACGASGQDAALYDTVSAVSHWCRDL
                     GLSIPVGKDSLSMRTAWQDEAGAQDVIAPVSLIVTAFAPVADARATLTPQLRTDMGDT
                     VLILVDLGKGRHRLGGSVLCQVFGQVGSIAPDIDDAGLLRSFFTAVRELAARGHLLAY
                     HDRSDGGLWATVCEMAFAGRVGVALNLDMLTIDPVAEDWGDFKIRPEQIQVQRDELTL
                     RALFAEEAGAVLQVRRDQRDDVLQHLRGAGLSAHSHVIGSLNGQDELQIYRDGQCVYR
                     KPRVELAQAWSEVSRRIMARRDNPACAQAEFDLWQDTQDPGLSPVVAFDPQENIAAPY
                     IASGKRPKVAILREQGCNSQVEMAWAFDTAGFEALDVHMTDLLSGRVRLEDAHGLVAV
                     GGFSYGDVLGAGEGWARTIRFNPALADQFAAYFARQDTFALGVCNGCQMMAALATMIP
                     GASHWPRFTRNVSEKYEARLSLVEVADSPSILFRGMAGARLPVAVAHGEGFADFSRQG
                     DAAQALGALHFVDNRGQRTEQYPLNPNGSPNGLTGVTTDDGRFTVLMPHPERVTRNAM
                     LSWAPARWGEQDTGGDASPWLRMFRNARVWVG"
     gene            complement(5316..5891)
                     /gene="greB"
                     /locus_tag="VFR20_13585"
     CDS             complement(5316..5891)
                     /gene="greB"
                     /locus_tag="VFR20_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEU0231593.1"
                     /translation="MNKAFVKESEDDDDDGAGAPAASLPSGVKNYMTTQGYRRLRDEL
                     THLMNEERPAVVQIVSWAASNGDRSENGDYIYGKKRLREIDRRMRFLTKRLEIAEVVD
                     PAAQPNREQVFFGATVVYSDSAGEEFRVTIVGVDEAEPLQGRISWISPVARALLKARE
                     GDTVQLRTPAGLDELDVLEVRYPAPGQGSDG"
     gene            complement(5927..6778)
                     /locus_tag="VFR20_13590"
     CDS             complement(5927..6778)
                     /locus_tag="VFR20_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006395761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU0231594.1"
                     /translation="MKPAITLNADLHCHSVHSDGVLTPVELARRAHGNGVRLWALTDH
                     DEVAGVAEAAAAAAALGMSFVPGVEISVTWAGRTVHIVGLRFDPANPALTAGLAGIRV
                     GRAERATEIGRRLDARLGMSGCLEGALRYAMNPGLVSRTHFARFLVEQGRFPTMQSVF
                     DQHLGQGKPANVPGRWAGLAEAVAWITGAGGRAVIAHPGRYAYSQLEYGAFFDAFKEA
                     GGEGLEVVTGSHRPAQFDEYAKVARHYGFLASRGSDFHSPGESRVDLGTLPPLPEDLT
                     TVWYDWF"
     gene            complement(6783..7709)
                     /locus_tag="VFR20_13595"
     CDS             complement(6783..7709)
                     /locus_tag="VFR20_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0231595.1"
                     /translation="MTEPRLEFVTCASPAGLHRMAYWEWGDPDNDRVLMCVHGLTRTG
                     RDFDTLAQRLCGTYRVVCPDIVGRGASDWLVNPDGYVVPQYVADLLTLIARSRPSRLD
                     WLGTSMGGLIAIALLGAQAMARATRPETVVHGLDAASGIPFGRIVLNDIGPDLDMGGM
                     ARIGLYVGQPVAFDTFDQAVDYVRDISPGFGHHTRQQWETLARRVMVQKGRQWIKHYD
                     LGMAKPFALQNEAALRASEALLWSAYESIQVPVLIVRGDCSDLLSTDTAARMLARNPR
                     AALHVVPDVGHAPTFMSDEQIEPVRRFLVADD"
BASE COUNT         1389 a         2670 c         2559 g         1305 t
ORIGIN      
        1 caaagccttg gccgccgccg cggtggcgat gttgccaccc acgacttgaa tcttggggta
       61 gttcttcttg acccagcgca cgcgttcgat gacgccggcc gaatggccat gcgcggtgtc
      121 cacgacgatg acgtcgacac cggcggcggc cagtttttcg acgcgctctt cagtgccctc
      181 gcccacaccg accgcagcgc ccacacgcaa ctgcccctgg gcatccttgc aggcgatggg
      241 gtgttcggtg ttcttgacga tgtctttcac cgtggccagg ccgcgcaact ggaacttgtc
      301 gttgacgatg agcacgcgtt cgaggcgatg cttgtgcatc agtgactggg cttcgtccag
      361 cgtggcgcct tccttcatgg tgatgaggcg ttcctgaggc gtcatgatgt cgcgcagggg
      421 gacgtcgagc cggtcttcaa agcgcaggtc gcgattggtg acgatgccca ccagtttgcc
      481 ggcctcgacc accggcaagc cggaaatgcc gtgctggcgc tgcagggcga tggcgtcgcg
      541 caccttcatg gtgggcgtga ccgtgaccgg atcgatgacg ataccgaatt cgtgccgctt
      601 gacgcgggcg acttcacgcg cctgttcgtc ggccgtcagg ttcttgtgga tgatgccgat
      661 gccaccttcc tgggccatgg cgatggccag gcgcgactcg gtcaccgtgt ccatggcggc
      721 cgagacgagc ggaatgttca gggtgatgtc gcgggtgaag tgggtgacga gggaagtatc
      781 gcgaggcagg acctcggaaa aggcggggac cagcaacaca tcgtcgaagg tcagtgcttt
      841 cttgatgagg cgcattcgta agctccgaat ccaaagcaag attatacgcg ttcagggcct
      901 gttcacaccc aaaggtgccc gtggcaatag ccgtggctat cgcgaggatt cgcaacgcga
      961 cgacgacccc gggccgcaga tacatgcggc ccggtgacct gttcagggga tgcctagccc
     1021 acccagaccc gggcgttgcg gaacatgcgc agccacggac tggcgtcgcc gccagtatcc
     1081 tgttcgcccc agcgcgccgg cgcccacgac agcatggcgt tgcgggtgac gcgctcggga
     1141 tgcggcatca gcaccgtgaa gcgcccatcg tccgtggtga cgccggtcag tccattcggg
     1201 ctgccattgg ggttgagcgg atactgctct gtgcgctgcc cgcgattgtc gacgaagtgc
     1261 agtgcgccca gcgcctgcgc ggcatcgccc tggcgcgaga agtcggcaaa gccctcgccg
     1321 tgcgccacgg ccaccgggag gcgggcgccc gccatgcccc ggaacaggat ggagggcgaa
     1381 tccgccactt ccaccaggga caggcgcgcc tcgtatttct cggagacatt gcgcgtgaag
     1441 cgtggccagt gcgaggcgcc cggaatcatg gtggcgagcg cggccatcat ctggcaacca
     1501 ttgcacaccc ccagcgcgaa cgtatcctgg cgggcgaaat aggccgcgaa ctggtccgcc
     1561 agcgcggggt tgaagcgaat ggtgcgcgcc cagccttcgc ccgcgcccag cacgtcgcca
     1621 tagctgaagc cccccacggc aaccagccca tgcgcgtctt ccaggcgcac gcgtccagag
     1681 agcaggtcgg tcatgtgcac gtccagcgcc tcgaagccgg ccgtatcgaa tgcccaggcc
     1741 atttcaacct gactgttgca gccctgctcg cgcagaatgg cgaccttggg acgcttgccc
     1801 gaggcgatat acggcgcggc gatgttttcc tgtggatcga acgccaccac gggcgacaga
     1861 cccggatcct gagtgtcttg ccagagatcg aactcggcct gggcgcaagc cgggttgtcg
     1921 cgccgcgcca tgatgcgccg gctgacttcg ctccacgcct gggccaattc cacccgcggc
     1981 ttgcgataga cgcactggcc gtcacggtag atctgtagtt catcctgccc attgagcgag
     2041 ccaatgacat gcgagtgggc cgacaggccc gcgccacgca gatgctgcaa cacgtcgtcg
     2101 cgctgatcgc gccgcacctg cagcaccgcc cccgcctctt cggcgaacag cgcccgcagc
     2161 gtgagttcgt cgcgctgtac ctggatctgt tcgggccgga tcttgaagtc gccccagtcc
     2221 tcggccacgg gatcgatggt cagcatgtcc agattgagcg ccacacccac gcgcccggcg
     2281 aatgccattt cgcagaccgt ggcccacagg ccgccatccg agcgatcgtg gtaggccagg
     2341 aggtggcccc gcgccgcgag ttcgcgcacg gcggtgaaga agctgcgcag caggccggca
     2401 tcgtcgatgt cgggcgcgat cgagcccact tgcccgaaca cctggcacag caccgagccg
     2461 cccaggcggt gccgtccctt gcccagatcg acaagaatga gcacggtatc gcccatgtcg
     2521 gtgcgcaatt gtggcgtgag cgtggcgcgc gcgtcggcca ctggcgcgaa cgccgtgacg
     2581 atgagcgaca ccggcgcgat gacgtcctgt gctccggcct cgtcctgcca ggcggtgcgc
     2641 atggaaaggg aatccttgcc cacgggaatg gacaaaccca ggtcgcggca ccaatggctg
     2701 acggccgaca ctgtgtcgta cagcgccgca tcctgcccgc tggcgccgca ggccgccatc
     2761 cagttggcgg acagcttgat gtcatgcagc gagccaacgt cggcggcaat caggttggtc
     2821 agcgcttcgg ccacggccag gcgcccggac gcggccgcgt cgatgaccgc caccggggtg
     2881 cgttcgccca tggccatggc ctgcccgcgc gtggtctcga agcccgacaa ggtgaccgcg
     2941 caatcggcca caggcacctg ccatggcccc accatctggt cgcggctgct gagcccaccc
     3001 acggtgcggt cgccaatggt gaccaggaat gtcttgttgg ccacggtggg atggcgcaac
     3061 acacgcaatg cggcttcttc cagcgataca gccgatagat cgagcgactc gcccgcccct
     3121 ggctcacgcc gcacatcacg cgtcatgcgt ggaggctttc caagaatcac gtcaacgggc
     3181 acatcgacag ggatgatgtc gtcggcggat gccgcctcgg gcacggatcc actcacgcca
     3241 gggatgccac cgccacgcgt gaccttcagg cgtcgatcct cggtggccac ccccacgacc
     3301 gcgtaggggc agcgttcacg acgcgcaatg acgtcgaaac gcgccagatc ttgcggcaga
     3361 atcgccaaga catagcgttc ctgtgactcg ttgctccaga tttcagcggc ggagaggccc
     3421 gattcctcga cgtgcacctg cgccagatcg aagatcgcgc cgcgccccgc gtcgttcacc
     3481 agttcgggaa aagcattcga gaggccaccg gcgcccacgt catggatggc cacgatgggg
     3541 tttccagccc cttgctgcca gcagcggtcg atcacttcct gcccgcgccg ctgcatttcc
     3601 gggttgccgc gctgcacgga atcgaaatcg aggtctgcgc tgttggcgcc cacgctcatg
     3661 ctcgaggccg cgccaccgcc catgccgatc cgcatgcccg gcccgccaag ctggatcagc
     3721 agcgcccccg gcggaatcgg gtctttgtgc gattgggtat cgtcgatggt gcccaggccg
     3781 ccggcgatca tgatgggttt gtgatacccc cagacacggc cgccggctcg ttgctcatgg
     3841 ctgcggaaat agcccagcag attgggccgg ccgaactcgt tgttgaacgc cgcgcccccg
     3901 atgggcccgt cgatcatgat gtccagggcc gtggcgatgc gcgcgggcgg caccggcaga
     3961 tcggcggttt cccagggttc gggcgcgtcg tcgaagcgca gattggacac cgtgaagccg
     4021 gtcagccccg ccttgggctt cgagccgcgg ccagtcgcgc cctcgtcgcg gatttctccg
     4081 ccggcaccgg tggcggcacc ggggaacggc gcgatggcgg tcgggtggtt atgcgtctcg
     4141 accttcatga gcgtgtgcgc caccacctcg cggcgctcgt acacgcacgc atcgtccggg
     4201 ctggcggcca gtggtggccg ggcctggaac tgcgtgacgg gcccgcccgc catgacggcg
     4261 gcattgtccg aatacgccac caccgtgcct tttggctgcg ccgcatgcgt ggcgcggatc
     4321 aggccgaaca acgtgtgctc ttgctcgacg ccgtccacga cccactgcgc attgaagatc
     4381 ttgtgccggc agtgctcgct gttggcctgc gcgaacatca tcagctcgac atcggtgggg
     4441 tcgcgtccga gttggctgaa agaggactga aggtagtcga tttcgtcatc ggacagcgcc
     4501 aatcccagct cgcggttggc gcgcaacaac gcgtcacgcc cttgttccag cacggccacg
     4561 cgtcgcgtgg gtttgcccgg caacgattca aaaagcgcct ggccgtcgaa atcggcatcc
     4621 accaccgatt ccgtcatgcg gtcatggaga caggcggcga tcgccgcgag ttgatcgggt
     4681 tcgatcttgc gcacgcccag cagcccgcgg ttcggcgtca agatataacg gatgccgcgc
     4741 tccaccctgc gcacggagga caggccacag ttgtgggcga tgtcggtcgc cttgctggcc
     4801 cagggcgaca ccgtgcccgg acggggaatc acccgcaaca ccagatcgtg cctgcccgtg
     4861 tcggccaccg ccgcgcgctc accgtagtcg agcaatgcca ccaaccggtc gcggtcgccg
     4921 gtgttcagcg cggcgccgag ccagacgaaa tgctcgtaac tagcgtggac gcccgagatg
     4981 ggcagttcca ggcgggcgag tcgctgcaac aggcgttcac ggcgaaacgc cgatagagcg
     5041 ggcgaaccgg ggagtatctg gatggaagtc acgatgcggc gcggggcaga aagaggggaa
     5101 caggaggaag aggcatcatt ttagccggtt ggccaccccg gcccgcgaca ggtcttttcg
     5161 aagcgccaaa agcggggttc gctacacaac acaacagggg agcccgaatc gggctgccgg
     5221 tgggaacgat ttgccgtcag cctcaagaaa aacacaactg tcgccagcgg tggcataccg
     5281 cgcgaccgtg gactgaatgg ccgggagctc gacgcctatc cgtcggaacc ctgtcctggc
     5341 gccggataac gcacttccag cacgtccagt tcatccagcc ccgccggcgt gcgcaactgc
     5401 accgtgtcgc cctcacgcgc cttgagcagc gcgcgcgcga ccggcgaaat ccagctgatg
     5461 cgcccttgca agggttcggc ctcgtcgacg ccgacgatgg tgacgcggaa ttcctcgccc
     5521 gcgctgtccg aatagacaac cgtggcgccg aaaaacacct gttcccgatt cggctgcgcg
     5581 gccgggtcga ccacttcggc gatttcaagc cgcttggtca ggaaacgcat gcgtcgatcg
     5641 atttcgcgca ggcgcttctt gccgtagatg tagtcaccgt tctccgagcg gtcgccgttg
     5701 gaagcggccc aggaaacgat ctgcaccacg gctggccgct cttcgttcat gagatgcgtc
     5761 aattcgtcac gcaggcgcct gtagccctgc gtcgtcatgt agttcttgac gcccgacggc
     5821 agactggcgg ctggcgcacc ggcgccgtcg tcatcgtcgt cttcagattc tttgacaaaa
     5881 gccttgttca tgatgtgcca cgctggtact gccgctatat gccgcttcag aaccaatcgt
     5941 accagaccgt ggtcaagtct tccggcagcg gaggcagcgt gcccagatca acgcggcttt
     6001 cccccgggct gtggaaatcc gaaccgcgcg aagccagaaa cccgtaatgc cgcgccacct
     6061 tggcgtattc gtcgaattga gccgggcgat ggctgccggt gaccacttcc aagccttccc
     6121 cgcccgcctc tttgaaggcg tcgaagaatg cgccgtactc cagttgggaa tacgcgtaac
     6181 ggcctgggtg cgcgatcacc gcgcggcccc ccgcgccggt gatccaggcg acggcctcgg
     6241 ccaggcctgc ccaccgccca ggcacgttgg cgggtttgcc ctgccccagg tgctggtcga
     6301 aaacagactg catggtgggg aagcgcccct gctcaaccag gaaccgggcg aaatgcgtgc
     6361 ggctgaccag accgggattc atcgcatagc gcaacgcccc ctccaggcac cccgacatgc
     6421 ccagccgcgc atccagcctg cggccgattt cagtggcgcg ttccgcgcgg cccacgcgaa
     6481 taccggccaa acccgccgtc aacgccgggt ttgccgggtc gaaacgcagg cccacgatat
     6541 gcactgtgcg ccccgcccat gtgacggaaa tctccacgcc cggcacgaac gacatcccca
     6601 gggccgcagc cgcggcggcc gcctcggcca cccccgcgac ctcgtcgtga tcggtcaacg
     6661 cccatagccg cacaccgttg ccgtgcgcgc ggcgcgccag ttcaacgggc gtcagcaccc
     6721 cgtcggaatg cacggaatgg cagtgcagat cggcgttgag agtgatcgcc ggcttcatga
     6781 tgtcagtcgt ccgcgaccag gaagcgcctg acgggctcga tctgctcgtc ggacatgaat
     6841 gtgggcgcat gccccacgtc cggcaccaca tgcagtgccg cgcgcgggtt gcgcgccagc
     6901 atgcgcgccg ccgtgtcggt cgacagcaga tcggagcagt cgccgcgcac gatgagcacc
     6961 ggcacctgga tgctttcata cgcggaccac aacagggcct cggacgcgcg cagggccgcc
     7021 tcgttctgca gggcaaaggg cttggccatg cccaggtcat agtgtttgat ccactgccgg
     7081 cctttttgca ccatcacgcg ccgggccagg gtttcccatt gttggcgcgt gtggtgcccg
     7141 aatccggggg agatatcgcg cacgtaatcg accgcctgat cgaacgtgtc gaacgccacg
     7201 ggctgaccca catacaggcc gatgcgcgcc atgccaccca tgtcgagatc cggcccgatg
     7261 tcgttgagca cgatacggcc aaagggaatg ccggatgcgg cgtccagacc atgcaccacg
     7321 gtttccgggc gtgtggcgcg ggccatggcc tgcgcgccca gcaatgcgat ggcgatgagc
     7381 ccgcccatgg acgtgcccaa ccaatccagc cgggacggac gcgagcgcgc gatcagcgtg
     7441 agcagatcgg ccacatactg cggcacgaca taaccgtcgg ggttgacgag ccagtccgac
     7501 gcgccccgcc cgacgatatc cgggcagacc acccgatagg tgccgcaaag ccgttgtgcc
     7561 agcgtgtcga agtcgcgccc ggtccgggtc aggccatgca cgcacatcag tacacggtcg
     7621 ttgtccggat cgccccactc ccagtaagcc atgcgatgca gacccgcggg gctggcgcaa
     7681 gtgacgaatt cgaggcgggg ttctgtcatg aaactgggct cgttgtatgg ggaaatcggg
     7741 cgggcacgag aaggcggtgc accgcctcgt gggatcctct gaacaaatcg ccgcgtcgca
     7801 cgcatccacg gccgctttcc gctcgacttg cccagggaat ccttgagcca ttatagagaa
     7861 ccgcccgacg cgcgagcgaa tgccccatgc cgcaacggcg ccccggggca ccaagcccgc
     7921 aca
//
LOCUS       DASTNK010000187         3249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1104852, whole genome shotgun sequence.
ACCESSION   DASTNK010000187 DASTNK010000000
VERSION     DASTNK010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104852"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VFR20_13600"
     CDS             <1..396
                     /locus_tag="VFR20_13600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU0231596.1"
                     /translation="DLVVGLSAIAAFLGHVYPIFLRFKGGKGVATALGVLLALQPWLA
                     LATAATWLIVAYASRYSSLASVVAAVFAPVYYWFGGHIAWEFSTTLCIAIAVISVMLI
                     FRHKANIGRLLAGAESRIGAGKKNGQPEK"
     gene            complement(408..1448)
                     /gene="tsaD"
                     /locus_tag="VFR20_13605"
     CDS             complement(408..1448)
                     /gene="tsaD"
                     /locus_tag="VFR20_13605"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU0231597.1"
                     /translation="MIILGFESSCDETGVAAVCTERGLLAHALHTQIAMHRHYGGVVP
                     ELASRDHVRRVVPLTRSVLRQAGLTFQDVGAVAYTAGPGLAGALLVGASVAQSLAWAL
                     GLPAIPIHHLEGHLLSPLLADPAPTFPFVALLVSGGHTQLMRVDGVGRYHTLGETLDD
                     AAGEAFDKTAKLLGLGYPGGPALSKLAEDGDATRVELPRPMLHSHDLDFSFSGLKTAV
                     LTRLRKMEEGGGVTPPQRADLAAATEAAIVDVLAAKSLRALRDTGLDSLVVAGGVGAN
                     RHLRRQLLAATAERGGRVYFPPLDLCTDNGAMIAFAAAQRVRAGLADLAEDTHAFTVR
                     PRWDLQAVCVPE"
     gene            1596..2888
                     /locus_tag="VFR20_13610"
     CDS             1596..2888
                     /locus_tag="VFR20_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU0231598.1"
                     /translation="MLDRYFKLREHGTTARTEILAGVTTFLTMSYIIFVNPDILSSTG
                     MDKGALFVATCVAAAIGCLIMAFVANWPIGLAPSMGLNAYFAFTVVGQLGFTWQQALG
                     AVFISGVVFVIITITGIREWLIAGIAASLRSGIVAGIGLFLGIIALEIAHIVVPNKAT
                     IVGLGNLSNTTTLYAILGFFIIAALDALRVRGAILIGILAITLLSVITGHVQFNGFFS
                     MPPSLDPSFLKLDIAGALSKGFFHVIIVFVLVEIFDATGTMIGIATRAGLVTPESNGR
                     LGRALLADSTAILASSVLGTSSTTAYIESASGVQAGGRTGMTAVIIAILFLLALFLSP
                     LAAIVPGYATAPALLYVACLMLRELVHVNWDDITESVPAAIATITMPLTYSIAYGLAF
                     GFISYVAIKALSGRFRDIHLAALLVAVLFVIKFAFFPD"
     gene            3020..>3249
                     /locus_tag="VFR20_13615"
     CDS             3020..>3249
                     /locus_tag="VFR20_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU0231599.1"
                     /translation="MSTIELDQDSFQQAIQADKPLIIDFWAPWCGPCRSFAPTFEAAS
                     GQHDDIVFAKVNTEEQQEIAAGMGIRSIPTLMV"
BASE COUNT          573 a         1120 c          960 g          596 t
ORIGIN      
        1 gacctggtcg tggggctgag cgccatcgcc gccttcctgg gccatgtgta cccgatattc
       61 ctgcgcttca aaggcggtaa aggcgtcgcc acggcgctcg gcgtgctgct ggcgctccag
      121 ccatggttgg cgctggccac cgcggcaacc tggctcatcg tggcctacgc cagccgctac
      181 tcgtcgctgg catccgtggt cgccgccgtg ttcgcccctg tctattattg gttcggcggt
      241 catatcgcct gggaattcag caccaccctg tgcatcgcca tcgccgtcat cagcgtcatg
      301 ctcatctttc ggcacaaagc gaatatcgga cgcctgctgg ccggtgctga aagccggatc
      361 ggcgccggca agaagaacgg gcaaccggaa aaatagagct ggccacccta ctccggcaca
      421 cacaccgcct gtagatccca acgcgggcgc actgtgaagg cgtgggtgtc ctcggccaag
      481 tcagccagcc cggcgcgcac ccgctgcgcg gcggcgaagg cgatcatggc gccattgtcg
      541 gtgcataaat cgagcggtgg aaaatacacc cggccgcctc gttccgccgt cgcggcaagc
      601 agttgccggc gcagatgccg attggccccg acgccgcccg ccaccacaag actatccagc
      661 ccggtatccc gcagcgcccg cagggacttc gccgccagca cgtcgacaat ggcggcttcc
      721 gtggcggccg cgagatcggc tcgttgcggc ggggtgacgc cccccccttc ttccattttt
      781 ctcagtcggg tcaggacggc agtcttgaga ccgctgaaac tgaagtcgag gtcatggctg
      841 tgcagcatcg ggcgcggcag ttcaacgcgg gtggcgtcgc cgtcctcagc cagtttcgat
      901 agcgcggggc cgcccggata gcccagcccc agcagtttcg cggtcttgtc gaacgcctcg
      961 ccggctgcgt catcgagagt ttcacccagg gtgtggtagc gccccacacc atccacgcgc
     1021 atgagctgcg tgtggccgcc cgacaccagc aacgccacga agggaaatgt cggggcaggg
     1081 tcggccagca agggcgaaag cagatgccct tcgaggtggt ggatgggaat ggccggcagc
     1141 cccagcgccc aggccagcga ctgcgcgacg ctggcgccca ccagcagtgc ccccgcgaga
     1201 cccgggccgg ccgtgtatgc cacagcgcca acgtcctgga acgtcaatcc cgcctggcgc
     1261 agaaccgagc gtgtcagggg caccacccgg cgcacgtgat cccgggatgc gagttcgggc
     1321 accacccccc cataatgacg atgcatggcg atctgggtat gcagggcgtg cgccaacagg
     1381 ccacgctcgg tgcagaccgc cgccacccct gtttcatcgc aggagctttc aaaaccaaga
     1441 ataatcatgc gcgtaagtgt acaacccgaa atcctcgcct tagaattgtc accggctcgc
     1501 gtggcgcacc gaaaacatga atttcaacac gcgcggcact cgacacaaca ccaactcgaa
     1561 cacgtagcgt ttttcgcaca aaaaaaggaa gggacatgct cgatagatat ttcaaactgc
     1621 gcgaacacgg caccacggcg cgcaccgaga tcctggcggg ggtcactacc ttcctcacga
     1681 tgtcgtacat cattttcgtc aacccggaca tcctcagcag cacgggcatg gacaagggcg
     1741 cgctgttcgt ggccacctgc gtcgccgcgg cgatcgggtg tctcatcatg gcgttcgtcg
     1801 cgaactggcc aatcggcctg gcgcccagca tggggttgaa cgcatatttc gcgttcaccg
     1861 tcgtgggcca actgggtttc acctggcaac aagcgctggg agcggtcttc atctcggggg
     1921 tcgtgttcgt gatcatcacc atcaccggca tccgggaatg gttgatcgcg ggcatcgcgg
     1981 catcgctgcg atcagggatc gtagcgggca tcggcctgtt tctgggcatc atcgcccttg
     2041 aaatcgcgca tatcgtcgtg ccgaacaagg ccaccatagt tggcctgggc aacctcagca
     2101 acacaacgac cctgtacgcg atcctggggt ttttcatcat cgcggcgctc gacgcgctca
     2161 gggtccgtgg cgccatcctg atcggcatac tggccatcac ccttctatcg gtcatcaccg
     2221 gccacgtcca gttcaacggc ttcttctcca tgccacccag cctggatccc agctttctga
     2281 agttggacat cgcgggggcc ctgagcaaag ggttcttcca cgtcatcatc gtgttcgttc
     2341 tggtggaaat ctttgacgcc accggcacca tgatcggcat cgccacgcgc gccggcctgg
     2401 tcacaccaga gagcaacggt cgtctgggcc gcgccctgct ggccgacagc acggccatcc
     2461 tggccagctc ggtcctgggc accagcagca ccaccgccta catcgaaagc gcgtcgggtg
     2521 tgcaggccgg cggccgcacg gggatgacag cggtcatcat cgccatcctg ttcttgttgg
     2581 ccttgtttct gtcgccgctc gccgccatcg tgcccgggta cgcgacggcg ccggccctgc
     2641 tctacgtggc ctgcctgatg ttgcgtgaac tggtccacgt gaactgggac gacatcaccg
     2701 aatccgtgcc cgccgccatc gccacgatca cgatgccgct cacgtactcc atcgcctatg
     2761 gcctggcctt cggcttcatc tcctacgtcg ccatcaaggc cctgtccggg cgcttccgcg
     2821 acattcacct ggcggcgctg ctggtcgccg tcctgttcgt gatcaagttc gccttcttcc
     2881 ccgactgacc accgtctgtg tcggcccgcc ccggcccatg atccgccggg gccgcccggc
     2941 gggccggccc cgctcgctgt acacttgtct tttctgcaca ttgagcggct gtcacgccgc
     3001 tctttcattt ggggatagca tgagcacgat cgaactggat caagactcct tccaacaggc
     3061 catccaggcc gacaaaccct tgatcatcga cttctgggca ccctggtgcg ggccgtgccg
     3121 cagcttcgcc cccaccttcg aggcggcgtc cggccagcac gatgacatcg tcttcgccaa
     3181 ggtgaacacc gaagaacagc aggaaatcgc cgcgggcatg ggcatccgct ccatccccac
     3241 cctcatggt
//
LOCUS       DASTNK010000188        22592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1110561, whole genome shotgun sequence.
ACCESSION   DASTNK010000188 DASTNK010000000
VERSION     DASTNK010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 22592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22592
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110561"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFR20_13620"
     CDS             <1..198
                     /locus_tag="VFR20_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0231600.1"
                     /translation="IIVVFAVVVIGGMGSILGSIVTGLGLGVIEGLTKVFWPEASTTV
                     VFIIMVIVLLIRPAGLFGKMK"
     gene            195..1205
                     /locus_tag="VFR20_13625"
     CDS             195..1205
                     /locus_tag="VFR20_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0231601.1"
                     /translation="MKRQLAAYLLAIVVLLLLPVLGAYPIFIMKIMCYALFACAFNLL
                     LGYTGLLSFGHAAFLGSAAYAAGQAIAVWGFPTVPGIIFGTAVAAVLGLVMGALAIRR
                     SGIYFAMITLAMAQMVYFYFLQAPFTGGEDGLQGIPRGTFLGIDLNSDTHLYYLVVVI
                     FVLSYALIWRTVNSPFGQVLKAIRENEPRAISLGYNVNSYKLLAFVLSATLAGLAGAT
                     KSVVFVSATLSDATWGMSGLVILMTLIGGLGTFTGPVLGAIIIVMLENKVGEFGRFMA
                     QATGLQWFQSLGESVTIVIGLIFVICVMAFRRGIVGEILHAWHSRKPPNGHAPEAGTQ
                     PS"
     gene            complement(1624..2433)
                     /locus_tag="VFR20_13630"
     CDS             complement(1624..2433)
                     /locus_tag="VFR20_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0231602.1"
                     /translation="MVSIVNGTPAAARNGVSMVLRVGGLSARYGKVTAVNNANLSVAP
                     GSIVTVIGGNGAGKSTLLNSIMGVLPLTGHCTGSVVYQGGEVCSWPAERRVFDGISLV
                     PERRELFASMSVEDNLLLGGFRLYRMRQRGWRDTMEHVYELFPRLRERRMQQAGTLSG
                     GERQMLAVGRALMAKPRLLMLDEPSLGLAPRIVREIFQIISRLRRTGVSILLVEQNAR
                     AALQVADYGYVLETGEIVLEGPASDLIGNPRVIESYLGLGNAGGKRDQNQE"
     gene            complement(2435..4261)
                     /locus_tag="VFR20_13635"
     CDS             complement(2435..4261)
                     /locus_tag="VFR20_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEU0231603.1"
                     /translation="MNRILTCVIIVILGVLPLLAVTPEFWITQLNYIGLASLVVLGLV
                     LLTGIGGLTSFGQAAFAGIGAYTTAYLTTAAGWSPWATLLAGLAITFVVAYVLGAITL
                     RLSGHFLPLGTIAWCLSLYYLFGNLEFLGQYDGIAGIVPIRFFGWSLSSGRHIFYLIW
                     IVLLLSLWATHNLLDSRPGRAIRALRSGAGMAESFGVNMAGYKVIVFVYAALLASISG
                     WLYAHMQRAVSPSPFGLNYGIEYLFMAVVGGAASVWGAVAGSGLILVLKDQIQNVLPH
                     LLHTTANFELIAFGVLMILVIQYARDGVWPLLARAWSAITPQSRQVRAAPPEAEALPR
                     RERPQRGSLVLEVDAVRKEFGGLVAVNDIGFSLRAGEIMGLIGPNGAGKSTTFNLITG
                     VLPVTRGAVRFLGHGIESLPSREIARLGVGRTFQHVQLLPTMTVLENVALGAHLRRSV
                     GVASAALRADRRAEAQLLHEAATQLRRVGLQDHLYEQAGNLALGQQRILEIARALAAD
                     PVLLLLDEPAAGLRYKEKQALAGVLRQLRGEGMSILLVEHDMDFVMNLTDHLVVMDFG
                     TKIAEGLPADVQRDPAVLEAYLGGVDDELDDEPSAEPVAAGA"
     gene            complement(4269..5306)
                     /locus_tag="VFR20_13640"
     CDS             complement(4269..5306)
                     /locus_tag="VFR20_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0231604.1"
                     /translation="MNSTIVLILLQDGVLNGAIYALLGLALVLVFVVTRVIFIPQGEF
                     VAFGALTLAFMVNGKIPGTVHLLLIAGILTFLVELFCAARARSWRSLAGTALWTLALP
                     VALLLLAPVVVGKNTPLWIDVVFSLALVIPLGPMIYRLVFQPVAEASVLVLLIIAVAV
                     HFALTGLGLVFFGAEGWRTPPFISGDVHLGSVTWSAQTFFVLGTCAALIIALWLFFGQ
                     TLYGRALRATAVNRRGARLMGISTSLSGSLTFTMAAAIGALSGMLIAPVTTIYYDTGF
                     IIGLKGFVAAIFGGMVSYPVTAVGALFVGILEAFSSFWASAYKEVIVFTLIIPVLMWR
                     SFSARHVEDEE"
     gene            complement(5464..6639)
                     /locus_tag="VFR20_13645"
     CDS             complement(5464..6639)
                     /locus_tag="VFR20_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0231605.1"
                     /translation="MKSSSSPLFRLSVLAGALALTLPLAAFAQIKVGLTLSSTGPAAS
                     LGIPERNTVVLLPTEVDGQKIEYIVLDDGTDTTRAVKNMRKLISDDNVDVVVGSSVTP
                     SSLAMVDVAGETKTPMISVAASAKIVDPVTGPRKWVFKTPQNDGLMASALADAMQKQK
                     VKTLGFIGFSDAYGQSWLQEMTKYAAQKGIKITDAEKYGRSDTSVTGQVLKLIASHPD
                     AILIAGAGTPSALPQRELKSRGFKGIIYQTHGAANNDVLRVCGKACNGMYLPAGPLLV
                     ADQLPDSNPVKKTAMQYKNLYEAKYGAGTMNTFGGHVWDAGQLIVHAIPAALKTGAKP
                     GTPQFRSALRDALENIKNLPASQGVFTMSPTDHAGLDKRGRVMIEVIDGKWVYQPDL"
     gene            7050..7445
                     /gene="msrB"
                     /locus_tag="VFR20_13650"
     CDS             7050..7445
                     /gene="msrB"
                     /locus_tag="VFR20_13650"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU0231606.1"
                     /translation="MEKIHKSDTQWRAELSPDEYAVARQKHTERPFTGRYWDTRTTGI
                     YRCVGCGTPLFASDTKFDAGCGWPSYFEPLDPALVREEVDNSHGMTRTEVLCNVCDSH
                     LGHVFPDGPPPTGLRYCINSLSLKFEPNS"
     gene            7442..8020
                     /locus_tag="VFR20_13655"
     CDS             7442..8020
                     /locus_tag="VFR20_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEU0231607.1"
                     /translation="MKKFLFDLFPLILFFVAFKFSDIYGATAVAIAAGLVQFAWLKVR
                     RKPIEATHWINLTVIILFGGATLFFHNDVFIKWKPTVLYWVFAGILLVGKWVFRRNLL
                     EKVMGAQVALPQIAWNKMMFSWAGFFIVSGILNLYVAFSGHYTEAQWVNFKVFGLMIL
                     LLVFVVLQSVWLHRYMDDGAEPRRAGSKGRAS"
     gene            8017..8277
                     /locus_tag="VFR20_13660"
     CDS             8017..8277
                     /locus_tag="VFR20_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEU0231608.1"
                     /translation="MNASRENLIRARLASLEPQDLRIIDESHLHAGHAGNRDGANHYR
                     VIIASPRFKGLSPVARHRLVYDCLDDLMPVPIHALAIVASAV"
     gene            8316..9146
                     /locus_tag="VFR20_13665"
     CDS             8316..9146
                     /locus_tag="VFR20_13665"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0231609.1"
                     /translation="MKRLAVFAAACVIAAPVFAANVATVNGQPLTQDQFDQFVKLLVS
                     KGAQDSPELRAQVKEEMISRMVAVQAAKKAGIENDPEIKQEMAIAQQNILVRALMADY
                     LKKHPITDAQVQAEYDKIKQEQAGRKEYKVRHILVKDEKTADKITADIKAKKITFANA
                     AKKDSIDPGSGKNGGELGWGPASNYVPAFAQAVESLKKGQMTDKPVHTQFGWHIIQVE
                     DSRPVKFPDLADVKTQIEQMLHQQALAKYQEGLISAATIKEDSDKAAAPKAATDTPKK
                     "
     gene            complement(9343..11418)
                     /gene="ligA"
                     /locus_tag="VFR20_13670"
     CDS             complement(9343..11418)
                     /gene="ligA"
                     /locus_tag="VFR20_13670"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU0231610.1"
                     /translation="MTDPGPDALGRRVDELRATIQQHNFRYYVQDAPSVSDAEYDRLM
                     GELTQLELAHPELITPDSPTQRVGGAPLAAFNSVRHAIRMLSLGNAFEDDDIHAFDKR
                     VAETLRSAGLLGETDLVEYAAEFKFDGLAINLRYEQGRLAQAATRGDGQTGEDVTSNI
                     RTLRSVPLRLAPGFPDVLEVRGEVLMHRADFARLNQAQQARGEKMFVNPRNAAAGSLR
                     QLDPRITAGRPLRFMAYGWGEVLPVPWGQGGTAPAGESTPATHAEVLDWFERLGLPVD
                     RHRRVVSGAAGLLSFYRRTGEQRSGLPFDIDGVVYKVNSLDAQEVLGYVARAPRFAIA
                     HKFPAQEETTQLLGIDVQVGRTGAITPVARLAPVFVGGVTVTNATLHNEDEVHRKDVR
                     VGDTVVVRRAGDVIPEVVGPVPELRPASAEPFVMPTACPVCGSRIERLEDEAVSRCTG
                     GLFCAAQRKQSLTHAAGRKALDIEGLGEKLVDQLVDSGRVKSLADLFMLSVEELAAYD
                     RMGRKSAENLVAALEKARTPPLASLLYALGIRHVGETTARDMARRFGSIDAVMAADEE
                     QLLEVPDVGPVVAGSVRRFFAEPHNRDVVAALAAAGVHARPPEATAAGAGLTGKTLVL
                     TGTLPNWSRDEATRRILAAGGKVSGSVSKKTAYVVAGEESGSKLAKARDLGVPILDEA
                     GLISLLGGD"
     gene            complement(11415..12437)
                     /locus_tag="VFR20_13675"
     CDS             complement(11415..12437)
                     /locus_tag="VFR20_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HEU0231611.1"
                     /translation="MSDLQIGLISLGIVLILLVLVFNWWQDRRLRGRMREHFPEADQD
                     PLMGGLSPVVGRREPGLNLAPAIDGAPAEDAPDVDPHCEAVIDIVFAQPVSSEQLYAA
                     THSLRTVGSKPVRVFAKSEGGGHRANLRQGESYTSMQLAVLLANRSGPLTDIEWSHLW
                     TAAQALAERFDGVVEGPEQEQVIQHATQLDELCAGLDAQVGLALRLGGTRPVTDVTRQ
                     VKDAGFLSHGGHLAWLADSGTPRFVLLYDGVHVQDVQSAGVDRIDLLLDVPNSPADGQ
                     AFSRMASVGRDLAGRLNATLVDDQGQAVSEASDKAIDEQLRDMYGKLDQAGFPAGGER
                     AIRVFS"
     gene            complement(12485..16012)
                     /gene="smc"
                     /locus_tag="VFR20_13680"
     CDS             complement(12485..16012)
                     /gene="smc"
                     /locus_tag="VFR20_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU0231612.1"
                     /translation="MRLTQIKLAGFKSFVEPTVIPTPSQLVGVVGPNGCGKSNIIDAV
                     RWVLGETRASELRGESMQDVIFNGSGQRKPAARASVELVFDNSEGRAAGQWSTYSEIA
                     VRRVLTRDGTSSYLVNNQQVRRRDIHDIFLGTGLGARGYAIIGQGMINRLIEARPEEL
                     RIFLEEAAGVSRYKERRRETENRLSDTRENLTRVEDILRELGTQLDKLDAQAEVAGRY
                     RALQQDGEQKQQALWYLKERDAHDDRQRKALAIEQAQTELEASLADLRAGEAELESRR
                     QAHYAAGDAVHAAQGKLFEAGAQVSRLEAEIRHVVDSRNRLQARRDQLQKQLEEWGEQ
                     KIHCTEQIEQSRAEQETGCARTEEARARVDEAQLALPDVESRVRSAAGARDEMRRVLA
                     SVEQNLALAAQTQRDSDRQLQALETRRERLEQQRRDLDVPDMAEIQRLEGDLAAIGEQ
                     LELAQGQLLELEQQLPDLDRQRTEAHAAARDESQALGRLEARMAALAKLQEDVQQQGA
                     LQPWLDKHELSGLGRLWQKLHIEPGWEPALEAVLRERIAALEIHQLEHARAFAGPDAP
                     PARLAFYQMPPAGPIPTPPAGLVPLGTLLRITDPDLRSLLADWLGGVYTQADVSTALA
                     RRQDLPPGGCYVVKEGHLVDRHSVRFYAPDSEQAGMLARQQEIEHLRREIKAGQLMVD
                     EATARGARAETAWQQVSGAMAPARGRVGELTRRVHDLQLNHSRLSQQASQSSELEARL
                     RGDLAEIAQQAEELRAVREESESRFEQLDAELGEHQSRFADAEMAGEDLATEAEQARL
                     RLRDAERAVQECEFAERALQARIDDLARNRQLAEDQIGRAQAELEGLQGELFDLDETA
                     SRAGLQEALETRATLEERLSEVRIELDTLEAGLRGADERRVELERSLDPRRARITELQ
                     LQEQAARLAVEQFSEQLDARSVDREQLGRMLAEQSDEWRRTGWLQSEVQRISRQVEGL
                     GPVNLAALDELKTARERREFLDSQYRDLTDAIQTLEDAIRKIDRETRELLQETFDTVN
                     SHFGDLFPRLFGGGQAKLSITGEEILDAGILVMAQPPGKRNSTIHLLSGGEKALTAIA
                     LVFALFKLNPAPFCLLDEVDAPLDDANTERYASLVENMSDQTQFLFISHNKIAMHMAR
                     QLVGVTMQEQGVSRIVAVDIESALQMAEA"
     gene            16342..17646
                     /gene="lplT"
                     /locus_tag="VFR20_13685"
     CDS             16342..17646
                     /gene="lplT"
                     /locus_tag="VFR20_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HEU0231613.1"
                     /translation="MKKGFYLVMAAQALSSVADNALLIAAIALIADLHGPDWMAPMMK
                     WWFALAYVVLAAFVGAFADAYPKGRVMFLTNAIKILGCLLMFCYQSFGLATHHQLALV
                     FCAYTLVGIGAAAYSPAKYGIVTEMLEPDLLVKGNSWIEGLTVLSIIVGTVIGGVLIR
                     PDVSHALLSHPWIHHLAQTRAEAAILLIAVVYFLAAICNLLIPRTNAWYPKQQTNPAK
                     LIRTFGGYVRILWHDKIGQISLAVTTLFWGAGATLQFIVIEWGAQHLNYRLDQASILM
                     GVAAFGTVIGAVSAGKVPLRRALSVLPVGIAMGIVILLMPFIYFKWTAYALLLVIGAL
                     SGFFVVPLNALLQHRGHVLLSAGHSIAVQNFNEQLNILVMLAVYSLMLWLRLSIDTII
                     IIFGVMVAVLMLVFMRWNRRNHKADPSLNSQIGLHGHGQALN"
     gene            complement(17643..18593)
                     /locus_tag="VFR20_13690"
     CDS             complement(17643..18593)
                     /locus_tag="VFR20_13690"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU0231614.1"
                     /translation="MVEPSLPPPRVTALQRAWLTELGLAPSILARWLTPDANAPGIRA
                     GVSSPKSNGVSVASPGVGGATGGLRTISELLAQPSPAEPRSSPDKKDVAHRDSLMNAL
                     AQRGGLPEAESGTQATGYGSAPVVPVGDLAALREQVLQCRACALCDGRSQAVFGDGAL
                     ASVEWMVVGEAPGDYDDRIGRPFQGDAGALLTGMLAAMGVANDSVYFTNLIKCRPLGN
                     RPPSREEIAACLPYLRRQIALLAPRRILALGRLAAQILTGGGAELDQLRGAVHAFEGE
                     DGRRIPLVVTYHPASLLLQPQHKPEVWRDLMLARGVMAKA"
     gene            complement(18586..19869)
                     /gene="tsaB"
                     /locus_tag="VFR20_13695"
     CDS             complement(18586..19869)
                     /gene="tsaB"
                     /locus_tag="VFR20_13695"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU0231615.1"
                     /translation="MDAHLLALETSSRQCEVALLSSVHGQLEVRTLSYDGQGGNTENL
                     LPMADALLAQAGLGRDSLDVVAFGQGPGGFTGLRVACGVAQGIAMALNIPVVPVGSLQ
                     AAAARDVWVDGDVHGQPVRVVVRDARMDEVYLGAYRHVPQSDPEAEDWRVVQAPMLLG
                     KADVGVWLRRFRPDWRDAHGRPSPVRLLGDALDAYPGLRDLAGEPGLADLQVGAAWHS
                     CAEAVARLGLDGWRRRRAVSPDLAAPLYVRDRVAYTTAERGRGLGGNPRAIVLPNIAV
                     EAMTPAHLDDVAAIERAVQSFPWTRRNFQDGLESGYGAWVARRDGVVVGFFLTLYAPD
                     VAHLLLIGVAPDAQRQGVGECLLRHCETESLAHGLDRVILEVRPSNGKALAFYDKHGY
                     TEMSRRKDYYPTGRPEREDALVLEKNLAETVPDHG"
     gene            20239..20973
                     /gene="ompR"
                     /locus_tag="VFR20_13700"
     CDS             20239..20973
                     /gene="ompR"
                     /locus_tag="VFR20_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HEU0231616.1"
                     /translation="MNTPNQSLSRKILVVDDDPRLRDLLRRYLSEQGFNVFVAEDGKE
                     MSKLWQREHFDLLVLDLMLPGEDGLSICRRLRGGHDNTPIIMLTAKAEEIDRIVGLEM
                     GADDYLSKPFNPRELLARVNAILRRRGTEEHPGAPSQENESIEFGPYVLNLSTRTLTR
                     NNEPVPITTGEFSVLKVFARHPKIPLSRDKLMELARGREYEAFDRSLDVQISRLRKLV
                     EPNPSKPVFIQTVWGLGYVFVPDGGH"
     gene            21013..22413
                     /locus_tag="VFR20_13705"
     CDS             21013..22413
                     /locus_tag="VFR20_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0231617.1"
                     /translation="MPSPQRWLKKAASRLRLGLFSRSFLLLAALMLVSLGAWLQVFFG
                     MEEGPRAVQMAQRVATAVSITRSALIYAPTSVRPALLLDLATKESLRVQPREGADVMQ
                     PLPDSNYWRRVAYEIQKRLGPATKIEWTVNQTPGIWVSFKINDDHYWLVFDREQLGLT
                     AGIEWFGWGATALLLALIGAAVSVRFVNRPLAQLAKVAQQLARGENPSPLPEKGPLEI
                     REMNIAFNRMARDLRQTEADREIMLAGISHDLRTPLARMRLEIEMSAVDDEARQAIDE
                     DLGQIDHSIGQLMEYARPAGVVPEQGTDVSEALRNLYERERSHTESLGGELTASIEPG
                     LRAQITAHDLKRVVGNLIENARRYGRSSSDGNPHIHLSLHDEDGLLVIQVIDQGVGIA
                     EADVERLLRPFSRGELARTGVSGAGLGLAIVERLLRQVGGQLKLLSQPSHGLTARIEI
                     PRSRANIRNFQLDSNI"
     gene            22490..>22592
                     /locus_tag="VFR20_13710"
     CDS             22490..>22592
                     /locus_tag="VFR20_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEU0231618.1"
                     /translation="MKTVGDKLEPFKVTGVKPGFNQHEENGVSAFEDI"
BASE COUNT         4238 a         7646 c         6794 g         3914 t
ORIGIN      
        1 atcatcgtgg tcttcgccgt ggtcgtcatc ggcggcatgg gttccatcct gggatccatc
       61 gtcaccggcc tcggactggg cgtcattgaa ggcctcacca aggtattctg gcccgaagcg
      121 tccaccacgg tcgtcttcat catcatggtc atcgtcctgc tcatacgacc cgcgggcctg
      181 ttcggaaaaa tgaaatgaaa cgtcaactcg cagcctatct gctggccatc gtcgttctgc
      241 ttctgctgcc agtgctcggc gcctacccga tcttcatcat gaagatcatg tgctatgcgc
      301 ttttcgcctg cgccttcaac ctgctgctgg gctacaccgg gttgctgtcc ttcggccatg
      361 cggcttttct gggttcagcc gcctacgccg ccggacaggc cattgcggtg tggggctttc
      421 ccaccgtgcc gggcatcatc ttcggcaccg ccgtggccgc cgtgctgggt cttgtcatgg
      481 gcgccctggc catacggcgc agcggcatct acttcgccat gatcaccctg gccatggccc
      541 agatggtcta cttctacttc ctgcaggccc ccttcaccgg cggtgaggac ggcctgcaag
      601 gcattccccg cggaacgttc ctcggtatcg atctgaacag cgacacccac ctgtactacc
      661 tggttgtggt gatcttcgtc ctcagttacg ccctcatctg gcgcaccgtc aactcaccgt
      721 tcggccaggt gctaaaggcc atacgcgaaa acgaaccacg cgccatctcg ctgggctaca
      781 acgtcaacag ctacaagctg ctcgccttcg tgttgtcggc cacgctggcg gggctggcgg
      841 gcgccaccaa gtcggtggtc ttcgtttcgg ccacgctgtc ggatgccacc tggggcatgt
      901 cgggcctggt cattctcatg actctcatcg gcgggctggg cacgttcaca ggcccggttc
      961 tgggggccat catcatcgtc atgctcgaga acaaggtggg cgagttcggc cgtttcatgg
     1021 cgcaagccac gggcttgcag tggttccaat cactgggcga gtccgtcacc atcgtcatcg
     1081 gcctgatatt cgtcatttgc gtcatggcct tccgccgagg catcgtcggc gagatcctgc
     1141 acgcctggca ctcacgcaag ccaccgaatg gccacgcacc cgaggcgggt acgcagcctt
     1201 cctgaagtcg aataggtatc tgaaggcgct gtctatcgga gcttgatgcg ccaatcgtcc
     1261 ataaccccgg caatatcccc tccatgccta tcgcaatcgt agccccaggg tgggcgaagc
     1321 agtgcaggcc ggcgtttcgg ccagcgccgg gtgctgccgt agggaggcgg gggatgtcgg
     1381 cgggcgatgt ctgagtggaa ccgcgccgga acggcgcggt ggagcgagtt tcgcccgacg
     1441 cccgccggac gaggcagacc cgggtagtcg gggggcgcag cgccacgacc gctggacgcg
     1501 ccggcctgca ctgcttcgtc caccctgggg cgggttaata gaaccacccc gactctcaag
     1561 cccgaaagta aaagaaaccc tgctgcatat gacgcagtga tgcacagcgc ggtgcgccgc
     1621 ccatcactcc tgattctggt ctcgcttccc gcccgcgttg cccagcccca ggtagctttc
     1681 aatgactcgc gggttgccga tcaggtcgga cgccggccct tccaggacga tctcgccggt
     1741 ttcgagcaca tagccatagt cggcgacctg caacgccgcg cgggcattct gctcgaccaa
     1801 caagatggaa acccccgtgc ggcgcagccg cgagatgatc tggaagatct cccgcacgat
     1861 gcgcggcgcg aggcccaggc tgggctcgtc gagcatcagc aatcgcggct tggccatcag
     1921 ggcccggccc accgcgagca tctggcgttc gccgcccgat agggtacccg cctgctgcat
     1981 gcgccgttcg cgcagtcgcg ggaacagctc gtaaacgtgc tccatcgtgt cacgccagcc
     2041 acgttgacgc atccggtaca gacgaaaacc acccagcagc agattgtcct cgaccgacat
     2101 gctggcgaac aattcgcgcc gttcgggcac cagggaaata ccgtcgaaca cacgccgttc
     2161 ggctggccag gaacaaactt ccccgccctg gtacaccacc gatccggtgc aatgccccgt
     2221 gagtggcaac acgcccatga tgctgttgag caatgtggat ttacccgccc cattgccacc
     2281 gatgacagtg acgatgctgc cgggtgcgac cgacaggttg gcgttgttga ccgccgtgac
     2341 cttgccgtaa cgggccgaca agccgccaac ccgcaagacc atcgagacgc cgttccgcgc
     2401 ggctgcgggc gtaccgttca caatactgac catgtcatgc ccccgccgcc acgggctcgg
     2461 ccgatggctc gtcatccagt tcgtcatcca cgccacccag gtaagcctcc agtaccgcgg
     2521 ggtcgcgttg cacgtcggcc ggcaagccct cggcgatctt ggtgccgaaa tccatgacca
     2581 ccaggtgatc ggtcagattc atgacaaaat ccatatcgtg ctcgaccaac agaatgctca
     2641 tgccttcacc gcgcaattgc ctgagcacac cagccagcgc ctgtttttcc ttgtagcgca
     2701 ggccggcagc aggttcgtcg agcagcaaca gcaccgggtc ggcggctagc gcgcgcgcga
     2761 tctcaaggat gcgctgttgg cctagcgcca ggttgccggc ctgttcgtac agatgatcct
     2821 gcaaacccac gcgacgcaac tgggtggcgg cctcatgcag caactgcgcc tcggcccgcc
     2881 tgtcggcgcg cagcgccgcc gacgccacgc ccacggaacg gcgcagatgc gcgcccagcg
     2941 ccacattctc gagcacggtc atggtgggta gcaattggac atgctgaaac gtgcgcccca
     3001 cacccagccg cgcgatctcg cgcgacggca aggattcgat cccgtgcccc aggaacctga
     3061 ccgccccgcg agtcaccggc agcacaccgg tgatgagatt gaaggtcgtg ctcttgccgg
     3121 cgccattggg cccgatcaac cccatgatct caccggcgcg tagcgaaaaa ccgatgtcgt
     3181 tgacggcgac caaaccgccg aattccttgc gcacggcatc cacctcgagc accaacgacc
     3241 cgcgttgcgg acgctcccgg cgcggcaacg cctcggcctc cggtggcgcg gcgcgcactt
     3301 gcctcgactg cggtgtgatg gccgaccagg cgcgcgcaag cagcggccac acgccatcgc
     3361 gcgcatactg gatgaccaat atcatcaaca cgccgaaggc gatgagctcg aaattggcgg
     3421 ttgtgtgcag caagtgcggc aacacgttct ggatctggtc tttcaggacg agaatcaggc
     3481 ccgagcccgc caccgcgccc catacgctgg ccgccccgcc caccaccgcc atgaacaggt
     3541 attcaatgcc gtaattcaac ccgaacggac tggggctgac cgcgcgctgc atatgggcgt
     3601 acaaccagcc cgagatgctc gccagcaagg ccgcgtacac gaagacaatg accttgtatc
     3661 cggccatgtt cacgccgaac gattccgcca tgcccgcgcc gctgcgcagc gcgcgaatgg
     3721 cgcgcccggg gcgcgaatcc agaagattgt gcgtggccca cagggacagc agcagcacga
     3781 tccagatcag atagaaaatg tgccggcccg agctcagcga ccacccgaag aaacggatgg
     3841 gcacaatgcc cgcgatgcca tcgtactgcc ccaggaattc gagattgccg aacaagtaat
     3901 acagcgacag gcaccacgcg atggtgccca gcggcagaaa gtggcccgac aaccgcagcg
     3961 tgatcgcacc caggacatag gccaccacga acgtgatggc caacccggcc agcaatgtcg
     4021 cccagggcga ccagccagcc gctgtcgtga gatacgctgt ggtgtaagcc ccgatgcccg
     4081 cgaaggccgc ctggccgaat gacgtgaggc cgcctatgcc ggtgagcagc accaggccca
     4141 gcaccaccag gctggcgagc ccgatgtaat tgagttgcgt gatccagaac tcgggcgtga
     4201 ccgccagcaa cggcagaacg cccagaatca cgatgatgac gcaggtaaga atccggttca
     4261 tggcgacgtt actcttcatc ctcgacgtgc cgcgcgctga acgagcgcca catcagcacg
     4321 gggatgatca gggtgaatac gatgacttcc ttgtaggcgc tcgcccagaa cgatgagaac
     4381 gcctcaagga tgcccacgaa cagggcccca acggcggtca cagggtagct gaccatgcca
     4441 ccgaagatcg ccgccacgaa acctttcaga ccaatgatga agccggtgtc gtaataaatg
     4501 gtggtgaccg gtgcgatcag catgccggac agcgcaccga tggccgcggc catggtgaac
     4561 gtcaacgacc cggacaggct ggtgctgata cccatgagcc gggcaccgcg ccggttgacc
     4621 gcggtggccc gcaacgcacg cccatacagg gtttggccga agaacagcca caatgcgatg
     4681 atcagcgcgg cgcatgtgcc cagcacgaag aacgtctgcg cggaccaggt gaccgagccc
     4741 agatgcacat caccactgat gaacggcggc gtgcgccagc cctcggcgcc gaagaacacc
     4801 agcccgagcc ctgtcagggc gaaatgcacg gccacggcga tgatgagcag cacaagcaca
     4861 ctggcctcgg ccaccggctg gaacaccaga cggtaaatca tgggccccag ggggatgacc
     4921 agggccaggg agaacacgac atcgatccac agaggcgtgt tcttgcccac caccaccggc
     4981 gcaagcagga gcagcgcgac cggcagcgcc agcgtccata acgccgtgcc cgccagcgac
     5041 ctccaggatc gggcacgcgc cgcgcagaac aattcaacca ggaatgtcag gatgccggcg
     5101 atgagcagca gatgcaccgt gcccgggatc ttgccattga ccatgaaggc cagggtcagc
     5161 gccccgaacg ccacgaactc gccctggggg atgaagatca cccgcgtgac gacgaacacc
     5221 agcaccagcg caagccccaa cagcgcatag atggcgccgt tgagcacccc atcctgcagc
     5281 aatatcaaaa caatcgtcga attcatccgc tgtacaccac actaccgcga cacgcccccg
     5341 tccgcgacat cagcgggccg gcagacgaca aacgccggct tcacgggaag cggcgccaga
     5401 gggaaaaccg atatcgcccg ggcacggcgc accttgcggc gcgcgcggcc gggcgccgcg
     5461 gaatcacagg tcaggctgat acacccattt gccatcgatc acttcgatca tgactcgccc
     5521 gcgtttgtcg agcccggcat gatcggtggg actcatggtg aagacgcctt gcgaggccgg
     5581 cagattcttg atgttctcga gcgcgtcgcg cagcgccgag cggaactgcg gcgtaccagg
     5641 cttggcacca gtcttgagcg ccgccggaat ggcgtggacg atgagctgtc cggcatccca
     5701 gacgtgaccg ccgaacgtgt tcatggtgcc cgcgccgtac ttcgcctcgt acaggttctt
     5761 gtattgcatc gcggtcttct tgaccgggtt gctgtccggc aactgatcgg ccacgagcaa
     5821 cgggcccgcc ggcaggtaca tgccattgca ggccttgccg cagacacgca gcacgtcatt
     5881 gttggcggcg ccgtgggtct gatagatgat gcccttgaag ccacggctct tgagttcgcg
     5941 ctgcggcagc gccgatggcg tgcccgcgcc agcgatgagg atggcatcgg gatgcgaggc
     6001 gatgagcttg agcacttggc ccgtgacact ggtatcggag cgcccgtact tttcggcgtc
     6061 cgtgatcttg atgccttttt gggccgcgta tttggtcatt tcctgcagcc agctctggcc
     6121 atacgcgtcg gagaaaccaa tgaaacccag ggtcttgacc ttctgcttct gcatggcgtc
     6181 ggccagtgcg gaagccatga gaccgtcgtt ctgcggggtc ttgaacaccc acttgcgcgg
     6241 cccggtgaca gggtcgacga tcttggcgct ggccgccacg ctgatcatgg gcgtcttggt
     6301 ttcccccgcg acatcgacca tggccaacga actgggcgtg accgacgagc caaccacgac
     6361 gtcgacgttg tcgtcactga tgagcttgcg catgttcttc acggcgcgcg tcgtgtcggt
     6421 accgtcgtcg agcacgatgt actcgatctt ctggccatcg acttccgtgg gtaacagcac
     6481 caccgtgttg cgctcgggga tgcccagcga cgcggcgggg ccggtgctgg acagcgtgag
     6541 cccgaccttg atctgggcga atgccgccaa gggcagcgtg agggcgagtg ccccggccag
     6601 aacggacaac cggaataagg gactggaact gctcttcatg tgggtctccg acgattaaac
     6661 gatgtgccga ccggcacgcg ggcagaaaga gaaaaaccga aacatcttac aaaaaatttc
     6721 gcgatcagca tatatttgta tcaaatgaag ccgaaaaaaa atccgactcc ggaaaattag
     6781 tttaggtttc tatcattcca tgaccagcac gccgattatc gccaacagcg gggacaaagt
     6841 cttttagggg attccctgcg tgccctgttg cctgcgcctg ggacacgccg cccccgcgcc
     6901 ccgccccagc cccggatacg aaaagaaaaa cattcgccat gacaacacca caaccatccc
     6961 ggcgggaacc aaccggggcc ggaaaagtcg attactatgg cgtatattga cggccatccg
     7021 gcaccagagg tatcactcag gccctcacca tggaaaaaat ccacaaatcc gacacccagt
     7081 ggcgcgccga gctcagcccg gacgaatacg cagtggctcg gcaaaagcac acagagcgac
     7141 cgttcaccgg gcgctactgg gacactcgca cgaccggcat ttatcgctgc gtgggctgcg
     7201 gcaccccgct tttcgcgtct gacacgaaat tcgacgcggg ctgcggttgg cctagctatt
     7261 tcgagcccct cgatccggcg ctggttcgtg aggaagtcga caacagccac ggcatgacac
     7321 gcaccgaggt tctgtgcaac gtttgcgact cgcatctggg ccatgttttc cccgatgggc
     7381 cgccccccac cgggttgcgt tactgcatca actcgctgtc actgaaattc gaaccgaact
     7441 catgaagaaa ttcctgttcg acctgttccc gctgatcctg tttttcgtcg cgttcaagtt
     7501 ctcggacatt tatggcgcga cggcggtcgc catcgccgcc gggctggtgc agttcgcctg
     7561 gctcaaggtg cgccgcaaac ccatcgaagc cacacactgg atcaacctca cggtcatcat
     7621 cctgttcggc ggcgccacgc tgttcttcca caacgatgtc ttcatcaaat ggaagcccac
     7681 cgtgctgtac tgggtgttcg ccggcatcct gttggtgggg aaatgggtgt tccgccgcaa
     7741 tctgctcgag aaagtcatgg gcgcgcaggt cgccctgcct caaatcgcct ggaacaaaat
     7801 gatgttcagc tgggccgggt tcttcatcgt ttcgggcatc ctgaacctgt acgtcgcgtt
     7861 ttcgggccat tacacggaag cgcagtgggt caatttcaag gtcttcgggc tcatgatcct
     7921 gctgctcgtc ttcgtggtgc tgcagtccgt ctggctgcat cgctatatgg acgacggtgc
     7981 cgagccgcgg cgggccggtt ccaaggggcg cgcctcatga atgcatcgcg cgaaaacctc
     8041 atccgcgcgc gtctggccag cctcgaaccg caagacctgc gaatcatcga cgaatcccac
     8101 ctgcacgccg ggcacgcagg caaccgcgat ggcgccaacc attaccgggt gatcatcgcg
     8161 tcaccccgct tcaagggctt gagcccggtg gcccgccacc ggctggtcta cgattgcctc
     8221 gacgacctga tgcccgtccc catccacgcg ctggccattg tcgccagcgc ggtctaagct
     8281 ccaattcaac ccctttcccc aaacaaagga attacatgaa acgactcgcc gtattcgccg
     8341 ccgcctgcgt catcgccgcg cctgtcttcg cggccaacgt ggccaccgtg aacgggcaac
     8401 ccctgacgca agaccagttc gaccagttcg tgaaactgct ggtcagcaag ggtgcccagg
     8461 actctcccga gctgcgcgcg caggtcaagg aggaaatgat cagccgcatg gttgccgtcc
     8521 aggccgccaa gaaggccggt atcgaaaacg accctgagat caaacaggaa atggccatcg
     8581 cccagcagaa catcctggtg cgagccctca tggctgacta cctgaagaaa caccccatca
     8641 ccgacgccca ggtccaggcc gagtacgaca agatcaagca ggaacaagcg ggccgcaagg
     8701 aatacaaggt gcgccatatc ctggtcaagg atgagaaaac cgccgacaag atcaccgccg
     8761 acatcaaggc caagaaaatc acgttcgcca acgcggccaa gaaagactcc atcgacccgg
     8821 gcagcggcaa gaatggcggc gagctgggct ggggcccggc ttccaactac gtgccggcct
     8881 tcgcccaggc tgtcgaaagt ctgaagaaag gccagatgac cgacaagccc gtgcacacgc
     8941 aattcggctg gcacatcatc caggtggaag attcccgtcc ggtcaaattc cccgacctgg
     9001 ccgacgtcaa gacgcagatc gagcagatgc tgcaccagca ggccttggcc aagtaccaag
     9061 aaggcctgat cagcgccgcc accatcaagg aagacagtga caaggcggcg gctcccaagg
     9121 cggccaccga cacacccaag aagtaactgt cattgcattg aagaggggct ttcacacgaa
     9181 agcccctttt tcatgcgcca ccatccggca cgtgggtgag accatggccc gtgatatggc
     9241 gcgtcaagtc gggtcgattg acgccgtgac ggcggccgac gaagggcaac cgcccggagg
     9301 cgcggacgga ggttcggcac ggcggccgga ctccacgctg gctcagtcgc cccccagcag
     9361 gctgatcaac cccgcttcat ccagaatcgg cactcccaga tcgcgcgcct tggcgagttt
     9421 gctgcccgat tcctcacccg ccacgacata ggccgtcttt ttggacaccg agccgctgac
     9481 cttgccgccg gccgcgagaa tgcggcgtgt ggcctcgtcg cgcgaccagt tgggcagggt
     9541 gcccgtcaac accagggtct tgccggtcaa cccagcgcct gccgcggtgg cttcaggcgg
     9601 gcgggcgtga actccggcgg ccgcaagcgc ggcgacgacg tcgcgattgt ggggctcggc
     9661 gaaaaagcgg cggacggacc ccgccaccac cggcccgacg tcgggtacct ccagcaattg
     9721 ctcttcgtca gccgccatca cggcgtcaat tgacccgaat cgccgcgcca tatcacgggc
     9781 cgtggtctcg cccacatgcc ggatacccaa cgcgtacagc aggctcgcaa gtggtggcgt
     9841 gcgcgccttc tcgagcgccg ccaccagatt ctcggcggac ttgcgcccca tgcggtcata
     9901 cgccgcgagt tcttcgaccg acagcatgaa cagatcggcc agcgacttga cccggccgct
     9961 gtcgaccaat tgatcgacca gcttctcgcc caacccttcg atatccagcg ctttgcggcc
    10021 ggccgcgtgg gtgaggctct gtttacgctg cgcggcgcaa aacaggccgc ccgtgcaacg
    10081 agacacggcc tcatcctcca ggcgctcgat gcgcgacccg cacacgggac acgctgtcgg
    10141 catgacgaac ggctcggccg aggccggacg caactcggga accggcccga cgacctcggg
    10201 gatgacatcg cccgcgcggc gcaccaccac cgtgtcgccc acccgcacgt ccttgcggtg
    10261 cacttcgtct tcgttgtgca gcgtggcgtt cgtgacggtg acgccaccca cgaacacggg
    10321 tgccagacgc gccaccggcg tgatggcgcc ggtgcgaccg acctggacat ctatgcccag
    10381 aagctgggtg gtttcctctt gtgcggggaa cttgtgcgcg atggcgaaac gcggcgcgcg
    10441 cgccacataa cccagcactt cctgcgcatc gagcgagttg accttgtaca ccacgccgtc
    10501 gatgtcgaac ggcaatccgg aacgttgctc cccagttctt cggtagaagc tcagcaagcc
    10561 cgccgccccc gacacgacac gtcgatggcg atcaaccggc agccccaatc gttcaaacca
    10621 gtccagcacc tcggcatgcg tcgctggcgt cgattcaccg gcaggcgcgg tgccgccctg
    10681 gccccatggc acgggtagaa cctcgcccca gccataggcc atgaaccgta gcgggcgccc
    10741 agccgtgatt cttggatcga gttggcgcag gctgcccgcc gccgcattgc gcggattcac
    10801 gaacattttc tcgccgcgcg cctgctgcgc ctgattcaat cgcgcgaaat cggcgcggtg
    10861 catcaacacc tcgccgcgca cctcgagcac atcgggaaac cccggcgcca ggcgcagcgg
    10921 cacggaacgc agggtacgga tgttgctggt gacgtcctcg ccggtctggc catctccacg
    10981 cgtggcggcc tgtgccaacc ggccctgttc gtagcgcaga ttgatggcca gcccgtcgaa
    11041 cttgaactcc gccgcgtatt cgacgagatc ggtctcgccc agcaacccgg ccgaacgaag
    11101 cgtctcggcg acgcgcttgt cgaacgcgtg gatgtcgtcg tcttcaaaag cattgcctag
    11161 cgacagcatg cgtatggcat gacgcacgct gttgaatgcc gccagcggtg cgccacccac
    11221 ccgctgggtg ggggaatcgg gtgtgatcag ttcgggatgg gcgagttcca gttgcgtcag
    11281 ttcgcccatg agccggtcgt actcggcatc ggacaccgag ggcgcatcct gcacgtaata
    11341 gcggaagttg tgctgctgga tggtggcgcg cagttcgtcg acccgccgac cgagggcgtc
    11401 gggaccggga tccgtcacga aaacacccgg atggcgcgct ccccgcccgc cggaaacccc
    11461 gcctgatcga gcttgccata catgtcgcgc aattgttcat cgatggcctt gtcggacgct
    11521 tcggagaccg cctggccctg gtcatcgacc agcgtggcgt tcagacgtcc ggcaaggtcc
    11581 cggcccacgc tggccatgcg gctgaatgcc tgcccgtcgg cggggctgtt ggggacgtcg
    11641 agcaggagat cgatgcggtc cacccccgcc gactgtacat cctggacatg caccccatcg
    11701 tagagcagca cgaagcgggg cgtaccggaa tcagcgagcc aggccagatg cccgccatga
    11761 gacaggaacc ccgcgtcctt gacctgacgg gtgacgtccg tcaccgggcg ggtgccgccc
    11821 aggcgcagcg ccaaacccac ctgtgcatcc aggccggcac agagttcatc gagctgggtg
    11881 gcgtgctgaa tgacttgttc ctgctcgggc ccttcgacca cgccgtcgaa gcgttcggca
    11941 agcgcctgcg ccgccgtcca gaggtgcgac cactcgatgt cggtgagcgg ccccgagcgg
    12001 ttggccagca acaccgccaa ttgcatggac gtatacgatt cgccctggcg caggttggcg
    12061 cggtggccgc cgccctcgct cttggcgaat acccgcacgg gcttgctgcc caccgtgcgc
    12121 agcgaatgcg tcgcggcata gagttgttcg gacgacacgg gttgcgcgaa cacgatatct
    12181 atcaccgcct cgcaatgcgg atcgacgtcg ggcgcgtctt cggccggggc gccgtcgatc
    12241 gcgggcgcca gattcaggcc gggctcacgg cgcccgacca ccggcgacag cccgcccatg
    12301 agcggatcct ggtcggcctc ggggaaatgc tcgcgcatcc tgccccgcag ccgcctgtcc
    12361 tgccaccagt tgaacaccag caccagcagg atcagtacaa tgccaaggga gatcagtccg
    12421 atttgcagat cactcatttc agccccacag gtacattccg gaattccgca gttgtcgcca
    12481 gaccctatgc ctcggccatc tggagcgccg attcgatatc gaccgccacg atgcgcgaca
    12541 cgccttgttc ctgcatcgtg acgcccacca gttgccgtgc catgtgcatc gcgatcttgt
    12601 tgtgcgaaat gaacaggaac tgcgtctggt cgctcatgtt ttccaccagg ctcgcatagc
    12661 gctcggtatt cgcatcatca agcggcgcgt cgacttcgtc aagcaggcag aacggcgcgg
    12721 ggttgagctt gaacaacgcg aacaccaggg cgatggcggt cagcgctttt tcgccgcccg
    12781 acagaaggtg gatggtgctg ttgcgtttgc cgggcggttg cgccatgacc aggatgccgg
    12841 catcgaggat ttcctcgccc gtgatggaca gcttcgcctg gccgccgccg aacaaccgcg
    12901 ggaacagatc gccgaagtgt gagttcaccg tatcgaacgt ctcttgcagc agttcacgcg
    12961 tctcgcggtc gatcttgcgg atggcgtctt ccagtgtctg gatggcgtcg gtcaggtccc
    13021 gatactggga atcgaggaac tccctgcgtt cgcgagcggt cttcagttcg tcgagcgcgg
    13081 ccaggttcac gggcccgagg ccttccacct ggcgtgaaat acgctgcacc tcggactgca
    13141 accagcctgt gcgccgccac tcgtcggact gctcggccag catccgcccg agttgctcgc
    13201 gatcgacgct gcgcgcatca agctgctcgg agaattgctc gaccgccagg cgcgccgcct
    13261 gctcctgcaa ctggagctcg gtgatgcgcg cgcggcgagg atcgagggaa cgctcaagct
    13321 cgacgcgccg ctcgtcggcc ccgcgcaggc cggcctcgag cgtatcgagt tcgatgcgga
    13381 cttcgctcag gcgctcctcg agggtggcgc gggtttccaa ggcctcttgc aggcccgccc
    13441 gcgaggcggt ttcatccaga tcgaacaact cgccctgcag cccttcgagt tcagcctggg
    13501 cgcggccgat ctgatcctcc gccaactgtc ggttgcgcgc cagatcgtcg atacgcgcct
    13561 gtaacgcgcg ctcggcgaat tcacattcct gcaccgcgcg ttcggcgtcg cgcaggcgca
    13621 gacgggcctg ctcggcttcg gtggcgaggt cttcgccggc catctcggca tcggcgaatc
    13681 gggattgatg ctcgcccagt tcggcatcga gttgctcgaa acgggattcg gactcttcgc
    13741 gcacagcgcg cagctcttcg gcctgttgcg cgatttcggc caggtcgccg cgcaggcgtg
    13801 cttcgagttc gctggattgg ctggcctgct gagacagtcg cgagtggttg agttgcaggt
    13861 cgtggacgcg gcgggtgagc tcgcccaccc ggccacgcgc cggcgccatg gctccggaca
    13921 cctgttgcca ggcggtctcg gcccgggcgc cgcgcgccgt ggcctcgtcc accatgagct
    13981 gccccgcctt gatctcgcgg cgcaagtgct cgatttcctg ctgacgggcc agcatgccgg
    14041 cctgttcgga gtctggcgcg tagaaacgca cgctatggcg atcgacgaga tggccttcct
    14101 tgacgacgta acagccgccc ggcggtaaat cctgtcgccg cgccaacgcg gtggacacgt
    14161 cggcctgcgt gtagaccccg cccagccagt cggccagcag cgaacgcaaa tcgggatcgg
    14221 tgatgcgcaa caaggttcca agcggcacca gccccgctgg tggcgtcggg atcggaccgg
    14281 ctggaggcat ctggtagaac gccaggcgcg ccggtggcgc atcaggcccg gcgaacgcgc
    14341 gcgcgtgttc cagttgatgg atttccaggg cggcgatgcg ttcgcgcagc acggcctcga
    14401 gcgcgggctc ccatccgggt tcgatatgca gcttctgcca tagccttccc aaaccggaca
    14461 actcatgctt gtccagccat ggctgcaagg cgccctgttg ctgaacgtct tcctgtagct
    14521 tggccagcgc ggccatgcgc gcctccaggc gccccagcgc ctgggattca tcgcgcgccg
    14581 ccgcgtgcgc ctcggtgcgc tggcgatcca gatcggggag ctgttgctcc agttcaagca
    14641 actggccctg cgcgagttca agctgttcgc cgatcgcggc cagatcgcct tccaggcgct
    14701 ggatttccgc catatcgggc acatcgagat cgcggcgctg ctgttcgagg cgctcgcggc
    14761 gggtttccag cgcctgcaac tggcggtcgg aatcccgttg cgtctgtgcc gccagcgcca
    14821 ggttctgctc cacggacgcg aggacgcggc gcatttcatc gcgcgccccg gccgccgagc
    14881 gcacccgcga ctcgacatcg ggcagcgcca gttgcgcctc gtcgacccgg gcacgggcct
    14941 cttcggtgcg cgcgcaaccc gtttcctgct cggcgcggga ttgctcgatc tgttcggtgc
    15001 agtggatttt ctgttcgccc cactcttcca actgcttttg caactgatca cgccgcgcct
    15061 gcaggcggtt gcgcgagtcc accacgtggc ggatttcggc ttcgagacga ctgacctggg
    15121 cgccggcctc gaacaacttt ccctgggccg catgcaccgc atcgccggca gcgtaatgag
    15181 cctggcgccg ggattcgagc tcggcctcgc ctgcgcgcaa atcggccagt gaagcctcga
    15241 gctcggtctg ggcttgctcg atggcgagag ccttgcgctg gcggtcatcg tgcgcgtcgc
    15301 gctctttcag ataccacagc gcctgctgct tctgttcgcc gtcctgttgc aacgcccgat
    15361 agcggccggc gacttccgcc tgcgcatcga gcttgtcgag ctgcgtgcca agctcgcgca
    15421 ggatgtcttc cacacgcgtc aggttttcgc gcgtatcgga caggcggttc tcggtttcgc
    15481 ggcggcgttc tttgtagcgc gacacgccgg ccgcctcttc caggaagatg cgcagttcct
    15541 cgggccgtgc ctcgatcagg cggttgatca tgccttggcc gatgatggcg tagccgcggg
    15601 cacccagccc ggtccccagg aatatgtcgt ggatgtcgcg ccgcctgacc tgctgattgt
    15661 tcaccaggta gctgctggtg ccatcgcggg tcagcacgcg ccgcaccgcg atctcggaat
    15721 aggtgctcca ctgccccgcg gcccgccctt ccgaattgtc aaacaccagc tccaccgacg
    15781 cgcgcgccgc cggtttacgc tgaccggagc cattgaaaat gacgtcctgc atcgattcgc
    15841 cgcgcaactc cgaggcccgc gtctcgccca gcacccaccg cacggcatcg atgatgttcg
    15901 acttgccgca gccgttgggc cccaccacac ccaccaattg gctgggtgtg ggaatgacgg
    15961 tgggctcgac aaacgatttg aagccggcca gcttgatctg agtcagacgc acaataggcg
    16021 agtcacgaaa aaagaaggag tgaaaccgtg gcgccgcccc tgccggcgat catggaaacg
    16081 gcccttgaac aaccaagccg ggtcacgggg attcatgaat ggttcgtatg ataacgcacc
    16141 ccccgccgtc tccgcactgg aatcacagtg ggttgaatcc cggctttggc gcatttcgat
    16201 acagccctct ccgctgacgc tatactcact aacaattttt tgcggtttaa ttttagtttt
    16261 tgctcggccg gccggccatt cgggctggtc aggcatactg accacccgct ccctcgttgc
    16321 cccctcttcc tggatacccc attgaaaaaa ggcttttatc tggtcatggc ggcccaggcg
    16381 ctttcttcgg tggcggacaa tgccctgctg atcgcggcca tcgcactcat cgcggacttg
    16441 cacggccccg actggatggc tcccatgatg aagtggtggt tcgcgctcgc ctacgtggtg
    16501 ctggccgctt tcgtgggcgc attcgccgac gcctacccca aggggcgggt catgttcctg
    16561 accaacgcca tcaagatcct cgggtgcctc ctgatgttct gctaccaaag cttcggtctg
    16621 gccacgcatc accagcttgc gctggtgttc tgcgcctaca cgctggtcgg catcggcgcg
    16681 gccgcctatt cccccgcaaa atacggtatc gtcaccgaaa tgctggaacc cgacctgctg
    16741 gtcaagggaa acagctggat cgaagggctg accgtcctct ccatcatcgt gggcaccgtg
    16801 atcggtggtg tgctgatccg gcccgacgta tcccacgcgc tgctgtcgca cccctggata
    16861 caccatctgg cccagacacg ggccgaagcg gccatcctgc tcatcgccgt ggtgtacttc
    16921 ctggcggcca tctgcaatct gctgatccca cgcaccaatg cctggtaccc gaaacagcag
    16981 accaatcccg ccaagctgat ccgcacattc gggggctatg tgcggatcct gtggcacgac
    17041 aagatcgggc agatatccct ggccgtcacc accttgttct ggggtgcggg ggccacgctg
    17101 caattcatcg tcattgaatg gggcgcccag catctcaact atcggctcga ccaggcatcc
    17161 atcctgatgg gggtcgccgc ctttggtacc gtcatcggtg ccgtgagcgc gggcaaggtc
    17221 ccgctgcggc gcgcgctgtc ggtgctgccg gtcggcatcg ccatgggtat cgtgatcctc
    17281 ctgatgcctt tcatctactt caaatggacg gcgtacgcgc tgctgctggt gatcggcgcg
    17341 ctgtcgggat tcttcgtcgt gccattgaac gcgcttctgc agcatcgggg ccacgtgctg
    17401 ctgtcggcgg ggcactccat cgccgtgcag aatttcaacg agcagctcaa catcctggtc
    17461 atgctggccg tctacagcct gatgctgtgg ctgcggctgt ccatagacac gatcatcatc
    17521 atcttcggcg tgatggtcgc ggtgctgatg ctggtgttca tgcgctggaa ccggcgcaat
    17581 cacaaggccg accccagcct caacagtcag atcggcctgc acggccacgg ccaagcgctg
    17641 aattaggcct tggccatgac accgcgcgcc agcatcaggt cacgccagac ctcgggcttg
    17701 tgctgaggct gcagcagcag cgacgccgga tgatacgtga ccaccagagg aatgcgccgg
    17761 ccgtcttcgc cttcgaacgc gtgtaccgcg ccacgcagtt gatccagttc ggcgccgcca
    17821 ccggtcaata tctgggccgc gaggcgcccc agggccagga tgcgccgtgg cgccagcaag
    17881 gcgatctggc gccgcaggta cggcaggcag gcggcgattt cctcgcgtga cggcgggcgg
    17941 ttgcccaggg gacgacattt gatcagattg gtgaaataca ccgaatcgtt ggccacgccc
    18001 atcgccgcca gcatgccggt gagcaatgcg ccggcatccc cctggaaggg tcggccgatg
    18061 cggtcgtcgt aatcgccggg cgcctcaccc accaccatcc attcgacgga agccagtgcg
    18121 ccgtcgccaa aaaccgcctg actacgtccg tcgcacagcg cacaggcccg gcattgcaac
    18181 acctgctcgc gcagggcagc cagatcgcca accggcacga caggagccga tccgtacccg
    18241 gtggcttgcg taccgctctc cgcttcaggt aacccccccc tctgtgccag cgcattcatg
    18301 aggctatcgc ggtgcgcgac atctttctta tcaggcgaag atcgcggctc ggcgggcgaa
    18361 ggttgcgcaa gcagctcgga aatagtccgc agtccgcccg tcgccccccc caccccgggg
    18421 gaggcgacgg acacaccatt ggatttgggt gacgacaccc cggcgcgaat ccccggggcg
    18481 ttcgcgtctg gtgtcagcca gcgcgccagt atggacggcg ccagacccag ttctgtcaac
    18541 cacgcccgct gcagggccgt gacacggggc ggtggcaagg atggttcaac catgatccgg
    18601 gaccgtctcg gccaggttct tttccagcac cagggcgtcc tcgcgctccg ggcggccagt
    18661 ggggtagtaa tcctttcttc ggctcatctc ggtgtaacca tgcttgtcat agaacgccaa
    18721 tgccttgcca ttcgacggcc gaacctcgag gatgacacga tcgagcccat gagccaggga
    18781 ctcggtttcg cagtgccgca gcaagcactc gcccaccccc tggcgctggg catcgggcgc
    18841 gacgccaatc aaaagcaaat gggccacgtc gggcgcgtac aaggtcagaa aaaaacccac
    18901 caccacccca tcgcgccgcg ccacccacgc accgtaaccc gattccagcc catcctggaa
    18961 gttccgacgg gtccagggaa aggactgcac ggcgcgctcg atggccgcga cgtcatccag
    19021 atgagcgggc gtcatggcct ccacggcgat gttcggcagc acgatagccc tgggattgcc
    19081 ccccagccca cgcccgcgct cagccgtggt gtaggcgacc cggtcacgca catacagcgg
    19141 cgcggccaaa tcgggggaca ccgcgcgtcg acgtcgccag ccatccaggc ccagacgcgc
    19201 gaccgcctcg gcgcacgagt gccacgcggc gccgacctgc aagtcggcca atcctggctc
    19261 gcccgccaga tccctcagac ccgggtaggc atccagcgca tcgcccagca accgcactgg
    19321 cgatggccgc ccgtgggcat cgcgccaatc cgggcggaag cggcgcagcc agaccccgac
    19381 atccgccttg cccagcagca tgggggcctg cacgacccgc cagtcctcgg cttccggatc
    19441 ggactgggga acatggcggt aggcgcctag atagacctcg tccatgcggg cgtcccgcac
    19501 cacgacacgc acaggctgcc catgcacgtc gccgtccacc cacacatcgc gcgcggccgc
    19561 cgcctggaga gaccccaccg ggaccaccgg aatattcagt gccatcgcga taccctgggc
    19621 cacgccgcac gccacgcgca aaccggtgaa cccgccgggc ccctggccga acgcgaccac
    19681 atcgaggctg tcacggccca ggccggcctg ggcgagcaac gcgtcggcca tgggcagcag
    19741 attctccgtg ttgccgccct gcccgtcata cgacagggtc ctgacttcaa gctggccgtg
    19801 caccgaagac agcagcgcca cttcgcactg ccgcgatgac gtttccagag caagcaggtg
    19861 agcatccatg atgtgttttc agcgcgttgt gacgcacccg gcctcggcgc gatcgagggg
    19921 gtcggtgcgc ttccggatgc ggtcgacata tggcgaaccc catccggtga tgacctctat
    19981 tttaaggaaa aacccgaagc cgttccggcc ctcggatgcg ccatggcgcc acccgaccca
    20041 ggccttcggc ctccggcaaa aataagctcc cgaaaggtac cgtgtggtgc taagctaacg
    20101 caagcaaata ttacaaaacg gggcaaacac cacgattttc cggtgcgcgt gtaagccagt
    20161 ctctaccagg cacttaatat gtaatatcat gctacaaacc cctgttcggg ttcaccctag
    20221 cctgttacat tttccaccat gaacacgcca aatcaatccc tatcacgcaa aatcctcgtc
    20281 gtcgacgacg atccgcgtct gcgcgacctg ctgcgtcgct atctatcgga acagggcttc
    20341 aacgttttcg tggccgaaga cggaaaagaa atgagcaaac tctggcagcg cgagcatttc
    20401 gacctgctgg tgctcgatct catgctgccc ggcgaagacg gcctgtccat ttgccgccgc
    20461 ctgcgtggcg ggcatgacaa cacgcccatc atcatgctca ccgccaaggc cgaggaaatc
    20521 gaccgcatcg tgggcctgga aatgggcgct gacgactacc tctccaaacc attcaatccg
    20581 cgcgaactgc tggcccgcgt caacgccatc ctgcgccgcc gcggcaccga agaacaccct
    20641 ggcgccccca gccaggaaaa cgagtccatc gaattcggcc cctacgtcct gaacctgtcc
    20701 acgcgcaccc tcacccgcaa caacgaaccc gtgcccatca ccacaggcga gttttcggtg
    20761 ctcaaggtgt tcgcgcgcca tcccaagatc ccgctgtcgc gcgacaagct catggagctc
    20821 gcccgcgggc gagaatacga agccttcgat cgcagcctgg acgtgcaaat ctcgcgcctg
    20881 cgcaaactgg tcgaacccaa cccgtccaag ccggtgttca ttcaaacggt ctggggcctg
    20941 ggctatgtct tcgtacccga tggcgggcac tgagcctgac agcgtttccc acgcttcagc
    21001 cctggttttg taatgccatc cccgcaacga tggctgaaga aggcggcctc gcggttacgg
    21061 ctgggcctgt tcagccgctc cttcctgctc ctggccgccc tcatgctggt cagcctgggc
    21121 gcgtggctgc aggttttctt cggcatggaa gaaggcccgc gcgcggtcca gatggcccag
    21181 cgcgtcgcca cggcggtcag tatcacgcgg tccgcgctca tctatgcccc caccagcgtg
    21241 cgccccgcgc tgctgctcga cctcgccacc aaggaaagcc tgcgtgtcca accccgtgaa
    21301 ggcgccgacg tcatgcaacc cctccccgac tccaactact ggcggcgcgt ggcctacgaa
    21361 atccagaaac gcctgggtcc agcgaccaag atcgaatgga cggtcaatca gacacccggc
    21421 atctgggtca gcttcaagat caacgacgac cactactggc tggtgttcga ccgcgaacaa
    21481 ctgggcctga cggcgggcat cgaatggttt ggttggggcg ccacggcgct gctgctggcg
    21541 ctcatcgggg cggcggtcag cgtgcgcttc gtcaaccggc cactggcaca actggccaag
    21601 gtcgcccaac aactggcccg aggggaaaat ccttcgccgc tgccggaaaa agggccactg
    21661 gaaatccgcg agatgaacat cgcgttcaac cgcatggccc gcgacctgcg ccagaccgag
    21721 gccgatcggg agatcatgct ggcgggcatc tcgcacgacc tgcgcacgcc actggcacgc
    21781 atgcgcctgg aaatcgaaat gagcgccgtc gacgacgaag cgcgccaggc catcgacgaa
    21841 gacctgggcc agatcgatca cagcatcggg cagctcatgg aatacgcgcg cccggctggt
    21901 gtcgtgcccg aacaaggcac cgatgtgtcc gaggccctgc gcaacctgta cgaacgcgaa
    21961 cgcagccaca ccgaatcgct ggggggcgag ctcaccgcct cgatcgaacc cggcctgcgc
    22021 gcccagatca ccgcccacga cctcaagcgt gtcgtcggca atctcatcga gaacgcccga
    22081 cgctacggcc gttcctcgtc cgatggcaac ccgcacatcc acctgtccct gcacgacgag
    22141 gacggcctgc tggtcatcca ggtcatcgac caaggcgtgg gtatcgccga agccgacgtc
    22201 gagcgcctgc tgcgcccctt ctcgcgcgga gaactcgccc gcacgggggt cagcggcgcc
    22261 ggcctgggcc tggccatcgt cgagcgcctg ctgcgtcagg tgggcggcca actcaaactg
    22321 ttgtcacaac catcccacgg cctgacggcg cgcatcgaaa tcccgcgatc ccgggccaat
    22381 attagaaatt tccaattaga cagcaatatc taatagcgta caattacaat gaaagaggtg
    22441 cgcgccaaag gcggtgcgat tccccccttc ccaagagaca ggagcattca tgaagactgt
    22501 tggcgacaag ctggaaccct tcaaggtgac cggcgtgaaa ccgggcttca accagcacga
    22561 ggaaaacggc gtatcggcgt tcgaagacat ca
//
LOCUS       DASTNK010000189         3885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U2140
            k141_1665023, whole genome shotgun sequence.
ACCESSION   DASTNK010000189 DASTNK010000000
VERSION     DASTNK010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960612
            Sequence Read Archive: SRR25158494
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:28:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,745
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1665023"
                     /isolate="SMAG_U2140"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            651..1793
                     /locus_tag="VFR20_13715"
     CDS             651..1793
                     /locus_tag="VFR20_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU0231619.1"
                     /translation="MNRLTKISALTAVASLTMGLGTAVAASGPIKIGVQAPITGEYAA
                     EGQGIENGVKLLAKQQNAAGGLLGRKIKVIVCDDEGKPAQAAICARKLVNDGVTAVIG
                     TYTSGAALAAAPIYAAANVIQTSDGTSDELTQKGWKTFFRNAPPNSDEALFTAKYLVN
                     VRHDKRIVVLSDHSSYATGLADSVEKAVKADKGNIVAKDFINAGTQDYTAVLTKLKSM
                     NPDVLYFSGYYTDGGLIRAQMEQLGMKAAFVGGDANQNAAFAKIAGNAAKGAIIINVP
                     APQNLPYPEAKKFLADYIAAYGSAPPSIYTFTNADGLRAVFAAIKATKSAETSKLIPW
                     MHNLKKPFDGVTGPFKWDAHGERVGSPMSAFEVQADGSYKTVYPTK"
     gene            1902..2804
                     /locus_tag="VFR20_13720"
     CDS             1902..2804
                     /locus_tag="VFR20_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0231620.1"
                     /translation="MDFILQQLINGLTVGGIYALIALGYTMVYGVLKLINFAHGDLCI
                     LGAFIGLTTLTSGVFGGLSTPLLLVLAFIIAMIVVAVTGTLLDQFAYKPLRRAHRLSP
                     VVSALGASMLIENGIMLIWGPNVRVFPSNLLPNITWHIGGAYLSFVQLLIMVGAFVLM
                     LALYFFVHHTRLGTAIRATAIDQDAARLMGINVNRVIALVFIIGPVLGAVGGLFVGLY
                     YRQAYFTMGWSYGLNAFIAAIIGGIGNIPGAMLGGLLLGLFNAFAAGYVSSSWQSAIT
                     FALLIAILLVRPSGLLGERVAEKV"
     gene            2809..3813
                     /locus_tag="VFR20_13725"
     CDS             2809..3813
                     /locus_tag="VFR20_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0231621.1"
                     /translation="MDKQLTQTSAGSAAPAPSSPVAAKPSGGTRALRIIGPLLWCLGL
                     LIPVVLSPAWITVGGIFAIYAIVALSQDIVLGRAGMYDMGHAVYFGIGAYISAILSLS
                     FGWPVLWTIPLAIIVSAAFGAILSAPIVKLRGDYLLVVTIGFNAIFVLAMKNNLFGLT
                     GGPDGIFGIGGPSFFGHTFGSQSDLFYLNWVVLAIVLWLMRNLDRSALGRTFRYLKHD
                     ELAATTLGVNARKYKVAAFALAAGIAGVAGTLFAMQLSAVSPGSFQFTDSVVLFAIVL
                     VGGQGSVPGVLLGTAMMFVVPQIFTDLAQYRYLVFGIAMIVVMVLRPQGIWPARKGGV
                     "
BASE COUNT          708 a         1197 c         1143 g          837 t
ORIGIN      
        1 acagccccag gccgcccatg cgcgcggcgc gatgactcca gaggatccct aggggaaatc
       61 ccgctttcga caccgcacgg ggtaggggtt tgcactaggt ccgcgcgata gggctgtgtt
      121 ttttactatg ccgtaatcct cgtcaaggaa tagcggcttc gcgcaggtgt cggcccgtga
      181 tgacgtgcgg atgatatgac gtgtttttcg aaaaattagc gtccacaaag gatatgcaag
      241 ggtcaaagga aatggtttcc gctgtttctt gtatcagggt ttgtactgac aatcacagtg
      301 actatgcaga aataatcaac tgaaacgtgc caaaatggca agacacgctg tcagtgtcta
      361 tctatactgt tcagggtgga atgggttgca ctcgtaagtt tcagaaagtt atacttcgcg
      421 catccttcgg gttgtgcttg ttggttgtac ctgtggtatc acccgaaacg atggagtaac
      481 ccggaatgaa cctcactgcc atggcaggcg acgcagcacg ttggggctcg cacccatctt
      541 cccgtcgaga caagggcaag acgctccgcc ccagggaaga tatccatggc gcatatttct
      601 ctctacatat ataagtggct gttggatcag ttagatcagg agaatccaga atgaataggc
      661 tgacaaagat ttccgcgctc accgcggtgg cttcgctgac gatgggcctg ggcacggcgg
      721 ttgccgccag cggccccatc aagattggcg tgcaggctcc catcaccggc gaatacgcgg
      781 ccgagggcca gggtatcgaa aatggcgtga agctgcttgc caagcagcag aacgccgccg
      841 gcggattgct gggtcgcaag atcaaagtga tcgtgtgcga cgacgaaggc aagccggccc
      901 aggccgccat ttgcgcgcgc aagctggtca atgacggcgt cacagccgtc atcggcacct
      961 acaccagcgg cgcggccctg gccgcggcgc ctatctacgc ggctgccaat gtcatccaga
     1021 cttcggatgg caccagcgac gaactcaccc agaaaggctg gaagacgttc ttccgcaacg
     1081 cgccccccaa cagtgacgag gccttgttca cggccaaata cctggtcaac gtccggcatg
     1141 acaagcgcat cgtcgtgctc agcgaccatt ccagctacgc cacaggtctg gccgacagcg
     1201 tcgaaaaggc cgtcaaggcc gacaagggca atatcgtcgc caaggacttc atcaatgccg
     1261 gcacgcaaga ctacacggcc gtgctgacca agctgaagtc catgaatccc gatgtcctgt
     1321 acttctccgg ctattacacc gacggcggcc tcattcgcgc gcagatggag cagcttggca
     1381 tgaaggccgc cttcgtcggc ggtgacgcca accagaacgc ggcgttcgcc aagatcgccg
     1441 gcaacgcggc caagggtgcg atcattatca acgtgcccgc tccgcagaac ctgccatatc
     1501 ccgaagccaa gaaattcctg gccgactaca ttgccgctta tggttcggcg cctccgagca
     1561 tctacacatt caccaacgcc gatggtctgc gtgccgtgtt cgccgccatc aaagccacca
     1621 agagcgccga gacctccaag ctcattccgt ggatgcacaa cctgaagaag ccgttcgacg
     1681 gtgtcacggg ccccttcaag tgggatgccc atggcgaacg cgtcggcagc ccgatgtcag
     1741 cctttgaggt ccaggccgac ggtagctaca agacggttta cccgacgaag taatcacacc
     1801 ggcgcacggc gccgtgcatc acccgatgcg cggcgccgtg tcgttttcgc gcggctcctg
     1861 gccgcgcgtg ccgcgatgcg gctcatcacg gaacccgaca gatggacttt atccttcagc
     1921 agttgatcaa cgggctgacg gtcggcggca tctatgcgtt gatcgcgctc ggctacacca
     1981 tggtctatgg ggtgctgaaa ctgatcaatt tcgcccatgg tgacctgtgc atcctgggtg
     2041 ccttcatcgg cctgaccaca ctcacgtccg gcgtgttcgg cgggctgtcc acgccgctcc
     2101 tgctggtcct ggcattcatc atcgcgatga tcgtggtggc cgtcaccggc acgcttctcg
     2161 accaattcgc ctacaagccg ttgcgccgcg cgcatcgcct ctctccagtc gtctccgccc
     2221 tgggcgcctc gatgctgatc gaaaacggca tcatgctgat ctggggcccc aacgtacgcg
     2281 tgtttcccag caatcttctg cccaacatca cctggcacat cggtggcgcc tatctcagtt
     2341 tcgtgcagtt gttgatcatg gtgggggcgt tcgtcctgat gctggcgctg tatttcttcg
     2401 tgcaccacac caggctaggc acggccatcc gcgccacggc catcgaccag gacgccgcgc
     2461 ggctcatggg catcaatgtc aaccgcgtca ttgccctggt attcattatc ggtccggtcc
     2521 tgggtgcggt cggcggcctg tttgtcggcc tctactatcg ccaagcctat ttcaccatgg
     2581 gctggtccta tgggctcaat gcgttcatcg cagccatcat cggtggtatc ggcaacattc
     2641 cgggtgccat gctgggcggt ctattgctcg gcctgttcaa cgcgttcgcc gccggttatg
     2701 tgtcgagttc ctggcaaagc gcgatcacgt tcgcattgct gattgcgatt cttctggtca
     2761 ggccgtccgg gctgctgggc gagcgcgtcg cggaaaaggt gtaatccaat ggacaagcaa
     2821 ctcactcaaa cctcggctgg ctccgcggcg ccagccccct cgtcgcccgt tgccgccaaa
     2881 ccgtcgggcg gcacgcgggc gctgcgcatc atcggtccgc tgctctggtg cctgggcctg
     2941 ttgattccgg tcgtgctgtc cccggcctgg atcacagtgg gcggcatctt cgccatttat
     3001 gccatcgtcg cgctttccca ggatatcgtg cttgggcgcg caggcatgta cgacatgggg
     3061 cacgccgtgt atttcggcat cggcgcctac atcagcgcca tcctgtcgtt gtcgttcggt
     3121 tggcccgttc tctggacgat tcctctggcc atcatcgtat cggcggcgtt cggtgccatt
     3181 ctgtcggccc cgatcgtcaa gctgcgcggt gactatctgt tggtggtcac cattggcttc
     3241 aacgccattt tcgtgctggc catgaagaat aacctgtttg gcctcaccgg cgggccggat
     3301 ggcatttttg gcatcggcgg cccatcgttc tttggccaca cgttcggcag ccaaagcgat
     3361 ctgttctacc tgaactgggt ggtgctggcg atcgttctgt ggctcatgcg gaatctcgac
     3421 cgctctgcgc tggggcgtac cttccgctat ctgaagcacg acgagttggc cgccacgaca
     3481 ctgggtgtca acgcgcgcaa gtacaaggtc gccgcgttcg cgctggcggc cggtatcgcg
     3541 ggtgtggcgg gcacgttgtt cgccatgcag ttgtccgcgg tcagcccggg ttcattccag
     3601 ttcaccgatt cggtcgtgct gttcgccatc gttctggtcg gcggccaggg ttcggtgccc
     3661 ggcgtgttgc ttggcaccgc catgatgttc gtggtgcctc agatcttcac cgatctggcg
     3721 cagtatcgtt atctggtgtt cggcatcgcc atgatcgtcg tcatggtgct caggccgcaa
     3781 ggcatctggc cagcgcgcaa ggggggcgta tgaatcaggc agtatccacc acccagtcgc
     3841 ccgacagcgc tgaaaccctg ctcgagctga acggtgtcgg catca
//
